LOCUS       DASUFA010000001         8628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1260509, whole genome shotgun sequence.
ACCESSION   DASUFA010000001 DASUFA010000000
VERSION     DASUFA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260509"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="VFX87_00005"
     CDS             <1..1541
                     /locus_tag="VFX87_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993309.1"
                     /translation="APLLAQTPKHGGVLTVMQREELPQGVSILETSTIATVWPASPCF
                     NNLVYFDPARPMETPDTIVGELAEKWSWQDGYRNLVFFLRRDVKWHDGQPFTSRDVKF
                     TFDLVREAPEASAKLRLNPRKDWYANIEAIEAADPYTVVFRLKRPQPSILMMLASGQS
                     PVYPAHVPVAEQRNRCVGTGPFKFKEWRRGEFVEYVKNPDYFVKGRPYLDGLKYVIIS
                     ERGTRTAALQAGRLDTSAPGETTKNIADQLKAAVPGMVITRVGSLTSPNLLVNHARPP
                     FNDARVRRAVDLAIDRDGYIKGVLQGAAVAGSSLVPRPFGSWGLSEGELVTILGRPDG
                     RKTAARKLLAEAGYGPASPLRFEAHTRGIATYVDLASYVVSELRRVGVEITVKQLDSV
                     QWYGVTTRKEFQLGINISGFGVDDPDAILFENYTCASLRNYTSYCDEGVSRLIDQQSQ
                     ELDPKKRLAIVHEIQKKLTEAGARPTVAWGIDHFAHWPYVKGLVPHHSLYSFGRMQNV
                     WLDR"
     gene            complement(1546..2883)
                     /locus_tag="VFX87_00010"
     CDS             complement(1546..2883)
                     /locus_tag="VFX87_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX4993310.1"
                     /translation="MTVLAPLGMLGYGIPARSMERGLARDPAVLALDAGSTDPGPYYL
                     GAGVPFVNRRAAKRDLTMILDAAHQRRIPVLIGSAGGGGGRPHVEWTLDLYRELCRER
                     GYRFRTAVIDAEVDRGWLRAQVAGGRVRPLDHDRALTATDIDAATRIVAQMGVEPFLT
                     ALDAGAEVVIAGRACDAAVIAAFPIRAGFDPGLALHMGKILECGGAAAYPRHGSDSLL
                     GVIEDDAFTVEPPNPDKVCTVASVAAHSLYERANPYELHLPHGHIDLKATRFEASGDR
                     AVRVSGTRFVPAVTPTLKLEGVARVGFRTLSVAGVREPQLIANLDAYLANVRTRVADT
                     YPDGYRLMFHVYGRDGVMGPLEPVRQSRAHEIGLIIEVIADDPETSAAVLALARSVAL
                     HVTYPGRKAIAGNLAFPFSPSDLRAGEAYEFTIHHLVAVDDPGALFRVELIEG"
     gene            complement(2906..3232)
                     /locus_tag="VFX87_00015"
     CDS             complement(2906..3232)
                     /locus_tag="VFX87_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEX4993311.1"
                     /translation="MKTIGEIATFVTSKNAGPFLLTLDIVFPDEATYRRFVAGQPLDR
                     ATVARLYGISPETVVDIIEFEPAFAVKITLRRPWASGAVGETDVYGAQQHVPLAGHAI
                     PWPEPV"
     gene            3380..4387
                     /gene="dctP"
                     /locus_tag="VFX87_00020"
     CDS             3380..4387
                     /gene="dctP"
                     /locus_tag="VFX87_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX4993312.1"
                     /translation="MSRFLSVPLLVVLMLVVAGPVAAQPKPITWDFILNVGLTHPVAI
                     MEKEFVDDVKKRTNGQLDIVLRPPGELPYKLPEYLRVVGQGNVQMGDVYMGFLAGDTK
                     IGVLTGLPFLVGTADELKKVMVPLEPMIREDYARFGADILTWFTWPEQNFFGRGKPIT
                     TLDDFKGRKIRTTSPEQSELVKRLGGVPVSIPAPEVPSAAQRGVMEGVITAGFNLLGA
                     KWYDFTEWAYLPNIHIGGPAYILVNKQALAALPAEHRKALQEAAAALQVKMLREIPAR
                     EQSDLKTLETEHKVRLIQAKPEDVVRARKLMDGYWDEWAKQGGPKTVEALAVVKKTLG
                     R"
     gene            4388..4915
                     /locus_tag="VFX87_00025"
     CDS             4388..4915
                     /locus_tag="VFX87_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX4993313.1"
                     /translation="MARAGGPLAAAAAGLRAAAMAVSGAAVVGMMALVTFEVLLRTFA
                     NRSTLVADEMAGYLLVAMTFLGLAPSLRDGAFIRIDTYRARLRGGARRLLDVALVTVA
                     LAYAITVDWYLWDLLAGTWRLGTTSIQVSRTPLWIPQAIMAVGGLLLILELLVEMVVV
                     VTADEALPAGESKVA"
     gene            4912..6219
                     /locus_tag="VFX87_00030"
     CDS             4912..6219
                     /locus_tag="VFX87_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX4993314.1"
                     /translation="MSWGVLILVVCALLVGLLSSGLWIAFGLGITGVLLLLLHGGVRA
                     LAPIGTIAWDTVESFVLTSVPLFILMGEVILRAGLSNTFYRSMGLWFHGLRGRLFQSN
                     IVACAVFAAVSGSSVATAAAIGTVAIPEMTRRGYERRLLFGSLAAGGTLGILIPPSIP
                     MIIYGAMVDQSVERLFVGGVVPGLLLALLFMAFIWVRVTMTPGLVPAAETAPTWGERL
                     RSLPGVAPMVCLILLVLGSIWLGYATPTEAAAVGATAAMVLAALAGRLTWTAFRESVV
                     GTVRTTCMVLFIIVGAQILSYGLVRTGVSRALTAWVVGIGLSRWLLFAIIVLLYIFLG
                     CIIDGVSMMVLTIPVLYPIVIAAGFDPIWFGIVLVIMIELGAITPPVGLNMFVIQGLS
                     GGRPLAEIVWGSLPFGVLMLVEIVIVSMFPGLVTWLPSRLVGK"
     gene            6216..7109
                     /locus_tag="VFX87_00035"
     CDS             6216..7109
                     /locus_tag="VFX87_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX4993315.1"
                     /translation="MTIGPGARPRPSLRALLAEPGLLVVPGAYDVITARLIEQAGFPA
                     CYMTGSGVSISALGIPDVGLISFYEVLVRLEQIAGAVALPVIADGDTGFGNPLNVLRT
                     VAEFERRGAAAIQIEDQEMPKKCGHEPGRRLVPAEEMIAKIEAARAARSSPDFLIVAR
                     TDARTTLGLADAIARGRRFAEAGADVIFVESPESAEEMRAICSQIPAPTLANMVEGGR
                     TPILPQAELAAIGYKIAIYPNALTRIMARAGQRLLEELKSTGTTAGLADEMFTHGELW
                     DLFGRREWAALEERFVTRRTP"
     gene            7109..7999
                     /locus_tag="VFX87_00040"
     CDS             7109..7999
                     /locus_tag="VFX87_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4993316.1"
                     /translation="MFAAPPSTDTRVFARIPEAKLLEGPSFDRAGNLWVVDIPHGRVF
                     RVTPDGRPTRIAEYPGEPNGLKIHRDGRIFIADHEQGLLILDPASGVTRVALDRPYAE
                     RFKGLNDLLFARNGDCYFTDQGESDQRDPTGRLFRLRASGELDCLLDNVPSPNGLVLT
                     PDESILYLAVTRGNAIWRVPLAAIGGLTVGRLGLGRVGIWVQMSGGVGPDGMAMDVEG
                     RVAVAHPGMGSVWVFDRRGEPVRRVRCCEGSRPTNVAYGGPEMKTLYITEADSGTVQM
                     AELDVPGLPLFSHQGDASVR"
     gene            complement(7988..>8628)
                     /locus_tag="VFX87_00045"
     CDS             complement(7988..>8628)
                     /locus_tag="VFX87_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4993317.1"
                     /translation="IGVVTQLLDSPDTDASDRTAVREAAETLRRLSARVEDVDLPLLP
                     LAGAVFMALADGEAAAWHHRTLATRGSEYDPGTRRRLVTAALLPATLAHQAARARAAI
                     RAQLEGALARFDVLLAPTGPTPAPPIASAQAPITSRAEAGRRFFNRRSYTTPASLAGV
                     PALSLPCGFTTGGLPIGLQLIGRRLDEATLLRAARAYERATEWHARRPPLPH"
BASE COUNT         1211 a         2949 c         3174 g         1294 t
ORIGIN      
        1 cggctccgtt gctcgcgcag acccccaagc acgggggagt gctgacggtc atgcagcggg
       61 aagagctgcc ccagggcgtg tcgatcctcg agacatccac gatcgcgacg gtctggccgg
      121 cctcgccgtg cttcaacaac ctggtttact tcgatcccgc caggcccatg gagacgcccg
      181 acacgatcgt gggcgagctg gccgagaagt ggtcctggca ggacggctac cggaacctgg
      241 tcttctttct cagaagggac gtgaagtggc acgacggcca gcccttcacc tcccgggacg
      301 tgaagttcac cttcgacctc gtgcgcgagg cgcccgaggc ctccgcgaag ctgcgcctca
      361 atccgcggaa ggactggtac gcgaacatcg aggccatcga ggcggccgac ccttacacgg
      421 tggtgttccg gctcaagcga ccccagccct ccatcctgat gatgctggcc tcggggcaat
      481 cgccggtcta tccggcccac gtgccggtgg ccgagcagcg taaccgctgc gtggggacgg
      541 ggccgttcaa gttcaaggag tggcgacggg gcgagttcgt ggagtacgtg aagaacccgg
      601 actacttcgt caagggccgg ccgtacctgg acggcctcaa gtacgtcatc atcagcgagc
      661 gcggcacccg gaccgcggcc ctccaggctg gccgcctcga cacctcggcg ccgggcgaga
      721 ccaccaagaa catcgcggac cagctcaagg cggcggtgcc gggcatggtg atcacccggg
      781 tcggctcgct cacctcgccg aacctgctcg tcaaccacgc gcgcccgccg ttcaacgacg
      841 cgcgggtccg gcgcgccgtg gacctggcca tcgatcgcga cggctacatc aagggggtgc
      901 tgcagggcgc cgcggtggcc ggctcgtcac tcgtgccccg tcccttcggc tcgtggggcc
      961 tgagcgaggg cgagctcgtg accattctgg ggcgcccgga cgggcgcaag accgcggcgc
     1021 gcaagctcct cgccgaagcc ggctacggcc ccgcgagccc gctccgcttc gaggcgcaca
     1081 cgcgcggcat cgccacctat gtggacctgg cctcctacgt ggtgagcgag ctgcgccggg
     1141 tcggggtgga gatcactgtc aagcagctcg acagcgtgca gtggtacggg gtcaccaccc
     1201 ggaaggagtt ccagctcggc atcaacatct cgggcttcgg ggtggacgat cccgacgcga
     1261 tcctgttcga gaactacacg tgcgcgtcgt tgcggaacta caccagctac tgcgatgagg
     1321 gcgtgtcccg cctgatcgat cagcagtcac aggagctgga cccgaagaag cgcctcgcca
     1381 tcgtccacga gatccagaag aagctcaccg aggcgggcgc ccgcccgacg gtggcctggg
     1441 ggatcgacca cttcgcccac tggccctacg tgaagggtct ggtgccgcat cactcgctgt
     1501 acagcttcgg ccggatgcaa aacgtgtggc tggaccgcta ggcggtcacc cctcgatcag
     1561 ctccacccgg aacagcgcgc ccggatcgtc gaccgcgacc aggtggtgga tcgtgaactc
     1621 gtacgcttca cccgcgcgca ggtcggaggg cgagaagggg aacgcgaggt tgcccgcgat
     1681 cgccttgcgg ccggggtagg tcacgtgcag ggccaccgag cgcgcgaggg ccaggaccgc
     1741 ggcggaggtc tcgggatcgt cggcgatcac ctcgatgatc aggccgatct cgtgggcccg
     1801 gctctggcgc accggctcga gcggacccat gacgccgtcg cgcccgtaga cgtggaacat
     1861 cagccggtag ccgtcggggt aggtgtcggc gacgcgggtg cgcacgttgg cgagataggc
     1921 atcgaggttg gcgatgagct gaggctcgcg gacgcccgcg acggagagcg tgcggaagcc
     1981 gacgcgcgcg acgccttcga gcttgagcgt cggggtgacg gcggggacga agcgcgtgcc
     2041 cgagacgcgc accgcgcgat cgccggaggc ctcgaagcgc gtggccttga ggtcgatgtg
     2101 gccgtggggc aggtgtagct cgtagggatt ggcccgctcg tagagcgagt gcgcggccac
     2161 cgaggccacc gtgcatacct tgtccggatt cggcggctcg accgtgaagg cgtcgtcctc
     2221 gatgacgccg agcaggctgt ccgagccgtg tcgcgggtag gcggccgcgc cgccacattc
     2281 gaggatcttg cccatgtgca gcgcgaggcc cgggtcgaag ccggcgcgga tcgggaacgc
     2341 cgcgatgacc gcggcgtcgc acgcgcgccc ggcgatgacc acctcggcgc ccgcgtcgag
     2401 ggcggtcagg aacggctcca cgcccatctg cgccacgatg cgcgtggccg cgtcgatgtc
     2461 ggtcgcggtc agcgcgcggt cgtggtcgag cggccgcacc cggcccccgg cgacctgcgc
     2521 gcgtagccag ccgcggtcca cctcggcgtc gatcaccgcg gtgcggaagc ggtagccgcg
     2581 ctcgcggcag agctcccggt agagatcgag cgtccactcc acgtgggggc gtccgccgcc
     2641 cccgcccgcg gagccgatca gcaccgggat gcgccgctgg tgcgcggcgt cgaggatcat
     2701 ggtgaggtcg cgcttggccg cccggcggtt gacgaacggc acgcccgcgc ccagatagta
     2761 agggccgggg tcggtcgagc cggcatcgag cgcgaggacc gccggatcgc gggcgaggcc
     2821 acgctccatg gagcgcgcgg ggatgccgta accgagcatg ccgagcgggg cgaggacggt
     2881 catcggttgc atgcggaggg ctccctcaga cgggctccgg ccacgggatc gcgtggccag
     2941 cgagcgggac gtgctgctgg gccccgtaca cgtcggtctc cccgaccgcg ccggaggccc
     3001 acgggcggcg cagtgtgatc ttcaccgcaa aggcgggctc aaactcgatg atgtcgacga
     3061 cggtctcggg cgagatgccg tagaggcggg ccaccgtggc gcggtcgagc ggctgcccgg
     3121 ccacgaaccg gcgataggtc gcctcgtccg ggaagacgat gtcgagggtc agcaggaacg
     3181 gtcccgcgtt cttgctcgtc acgaacgtgg cgatctcgcc aatggtcttc atcgcgaggc
     3241 catcgcacca ctccgggccc tccgctgtca atagacggct tgacaaggcg gcgcgcccgg
     3301 ggctacaagg cgagtgccgc atcgggtacc gctgctgctc accccgagcc cggctcgcgc
     3361 cggagcgagg agatcctgca tgtcgcgctt cctctcggtc cccctgctcg tcgtcctgat
     3421 gctcgtggtc gccgggccgg tcgcggccca gcccaagccg atcacctggg atttcatcct
     3481 caacgtgggg ctcacgcacc cggtggcgat catggagaag gagttcgtgg acgacgtgaa
     3541 gaagcggacg aacggccagc tcgacatcgt gctgcggccg cccggcgagc tgccgtacaa
     3601 gctcccggag tacctgcgcg tggtggggca gggcaacgtc cagatgggcg acgtgtacat
     3661 gggcttcctg gccggcgaca ccaagatcgg ggtgctgacc ggcctgccct tcctcgtggg
     3721 cacggccgac gagctcaaga aggtgatggt cccgctcgag ccgatgatcc gagaggacta
     3781 cgcacgcttc ggggccgaca ttctcacgtg gttcacctgg cccgagcaga acttcttcgg
     3841 gcgcggcaag cccatcacga ccctcgacga cttcaagggc cggaagatcc gcaccaccag
     3901 ccccgagcag tccgagctgg tgaagcggct cggcggggtg ccggtctcga tcccggcgcc
     3961 cgaggtgccg agcgccgcgc agcgcggcgt gatggaaggc gtcatcaccg ccgggttcaa
     4021 cctgctcggc gcgaagtggt acgacttcac cgagtgggcg tacctgccga acatccatat
     4081 cggcgggccc gcctacatcc tggtcaacaa gcaggcgctg gccgcgctgc ccgccgagca
     4141 ccgcaaggcg ctccaggagg cggcggccgc gctccaggtg aagatgctgc gcgagatccc
     4201 cgcccgcgag cagagcgacc tcaagacgct cgagacggag cacaaggtca ggctgatcca
     4261 ggccaagccg gaggacgtgg tgcgcgcccg caagctgatg gatgggtact gggacgagtg
     4321 ggccaagcag ggcgggccca agacggtgga ggcgctcgcc gtggtcaaga agacgctcgg
     4381 ccgctagatg gcccgggccg gggggccgct ggccgccgcg gcggccggcc tgcgggcggc
     4441 cgcgatggcg gtgagcggcg ccgcggtcgt gggcatgatg gccctcgtga cgttcgaggt
     4501 gctgctgcgc acgttcgcga atcgctcgac gctggtggcc gacgagatgg ccggctacct
     4561 gctggtcgcg atgaccttcc tggggctggc cccgagcctg cgcgacggcg ccttcatccg
     4621 catcgatacc tatcgcgccc ggctgcgcgg cggcgcgcgc cggctgctcg acgtggccct
     4681 ggtcacggtc gccctggcgt acgcgatcac ggtggactgg tacctgtggg acctgctggc
     4741 cggcacctgg cggctcggca ccacgtccat ccaggtctcc cggacgccgc tctggatccc
     4801 tcaggcgatc atggcggtgg gcgggctcct cctcatcctg gagctgctgg tcgagatggt
     4861 cgtcgtggtt accgccgacg aggcgttgcc tgccggagaa tcgaaggtcg catgagctgg
     4921 ggcgtcctga tcctcgtcgt ctgcgcgctg ctggtgggcc tgctcagctc ggggctctgg
     4981 atcgcgttcg ggctcggcat caccggcgtg ctcctgctcc tgctccacgg cggggtgcgc
     5041 gcgctcgcgc cgatcgggac catcgcgtgg gacaccgtcg agagcttcgt gctgacgtcg
     5101 gtgccgctct tcatcctcat gggcgaggtg atcctgcgcg ccgggctcag caacaccttc
     5161 taccgcagca tggggctctg gttccacggg ctgcgcggcc gcctcttcca gtcgaacatc
     5221 gtggcctgcg cggtcttcgc ggcggtgagc ggctcgagcg tggcgaccgc ggccgccatc
     5281 ggcacggtgg cgatcccgga gatgacgcgg cggggctacg agcggcggct gctcttcggt
     5341 agcctcgcgg cgggcggcac gctgggcatc ctcatcccgc ccagcatccc catgatcatc
     5401 tatggggcga tggtggacca gtcggtggag cgcctcttcg tcggcggtgt ggtgcccggg
     5461 ctcctgctcg cactgctctt catggctttc atctgggtgc gcgtgacgat gacgccgggc
     5521 ctggtgccgg ccgcggagac ggcgcccacc tggggcgagc ggctgcgcag cctgcccggg
     5581 gtggccccga tggtgtgcct catcctgctc gtgctcggga gcatctggct cggctacgcg
     5641 acgcctaccg aggcggccgc ggtcggggcc accgcggcga tggtgctcgc cgcgctcgcg
     5701 ggccggctca cctggacggc gttccgcgaa agcgtcgtgg gcacggtccg gaccacctgc
     5761 atggtgctgt tcatcatcgt gggcgcgcag atcctgtcct acgggctcgt gcgcaccggg
     5821 gtgagtcggg cgctcaccgc gtgggtggtg ggaatagggc tctcccggtg gctgctgttc
     5881 gcgatcatcg tcctcctcta catcttcctc ggctgcatca tcgacggtgt ctcgatgatg
     5941 gtgctcacga tcccggtcct gtacccgatc gtgatcgcgg ccggcttcga tccgatctgg
     6001 ttcggcatcg tgctggtgat catgatcgag ctgggggcca tcaccccgcc ggtggggctc
     6061 aacatgttcg tcatccaggg cctgtccggc ggccggccgc tggcggagat cgtctggggc
     6121 tcgctgccct tcggagtgct catgctcgtg gagatcgtga tcgtttcgat gttccccggc
     6181 cttgtcacct ggctgcccag ccgtctggtg ggcaagtgac catcgggccg ggcgcgcgcc
     6241 cccgtccgag cctccgggcc ctgctcgccg agccggggct tctggtggtg ccgggggcct
     6301 acgacgtcat caccgcgcgc ctgatcgagc aggcgggctt cccggcctgc tacatgaccg
     6361 gctcgggcgt ctcgatcagc gccctgggga tccccgacgt cggcctcatc tcgttctatg
     6421 aggtgctggt gcgcctggag cagattgcgg gggcggtggc gctgccggtg atcgccgacg
     6481 gcgacaccgg cttcgggaac ccgctcaacg tcctgcgcac cgtggcggag ttcgagcgcc
     6541 gcggggcggc cgcgatccag atcgaggacc aggagatgcc caagaagtgt gggcacgagc
     6601 ccggccgccg tctcgtgccg gcggaggaga tgatcgcgaa gatcgaggcg gcccgggccg
     6661 cgcgctcctc gcccgacttc ctgatcgtgg cgcgcaccga cgcccgcacc acgctgggcc
     6721 tcgccgacgc gatcgcgcgc ggccgccggt tcgcggaggc cggcgccgac gtgatcttcg
     6781 tggagtcacc ggagagcgcc gaggagatgc gcgcgatctg ctcgcagatc ccggcgccga
     6841 ccctggccaa catggtggag ggcgggcgca ccccgatcct cccgcaggcc gagctggccg
     6901 cgatcggcta caagatcgcg atctacccga acgcgctgac ccggatcatg gcccgggccg
     6961 ggcagcgcct gctcgaggag ctcaagtcga ccggcaccac cgcgggcctc gccgacgaga
     7021 tgtttaccca cggcgagctg tgggatctct tcggtcgccg cgagtgggcc gccctggagg
     7081 agcgcttcgt gacccggagg accccgtgat gttcgccgcc ccgccctcga ccgacacgcg
     7141 tgtcttcgcc cggatcccgg aagccaagct gctggagggc ccgtcgttcg accgcgcggg
     7201 caacctctgg gtcgtggaca tcccgcacgg gcgggtgttc cgcgtgacgc ccgacggacg
     7261 gcccacgcgc atcgctgagt atccgggcga gccgaacggg ctgaagatcc accgagacgg
     7321 gcggatcttc atcgcggacc acgagcaggg gctgctgatc ctcgatcccg cgagcggcgt
     7381 cacgcgcgtg gcgctcgatc gcccctacgc ggagcggttc aagggtctca acgacctgct
     7441 cttcgcgcgc aacggcgact gctacttcac cgaccaggga gaatcggacc agcgcgaccc
     7501 gaccggccgg ctcttccgcc tgcgggcgag tggcgagctg gactgcctgc tcgacaacgt
     7561 gccgagcccc aacgggctcg tgctgacgcc ggacgagtcg atcctgtatc tcgcggtgac
     7621 gcgcggcaac gcgatctggc gcgtgccgct cgccgcgatc ggcggcctga cggtgggacg
     7681 gctcggcctc gggcgcgtcg ggatctgggt gcagatgtcg ggcggcgtcg gccccgacgg
     7741 catggccatg gacgtcgagg ggcgggtggc ggtggcgcat ccgggcatgg gctcggtgtg
     7801 ggtgttcgac cggcgcggcg agccggtgcg ccgggtgcgc tgctgcgagg ggtcgcggcc
     7861 cacgaacgtg gcctacggcg gccccgagat gaagacgctc tacatcaccg aggcggactc
     7921 gggcacggtg cagatggccg agctcgacgt gcccggtctc ccgctcttct cccaccaggg
     7981 cgacgcgtca gtgcggtagc ggcggccgcc gcgcgtgcca ctcggtggcg cgctcgtagg
     8041 cgcgggccgc gcgcagcagc gtcgcctcgt cgaggcgccg gccgatgagc tgcaggccga
     8101 tcggcagccc gcccgtggtg aacccgcagg gcagcgagag cgcgggcacg ccggccaggc
     8161 tcgcgggcgt ggtgtaggag cgccggttga agaagcgccg cccggcctcg gcgcgcgacg
     8221 tgatcggcgc ctgggcgctg gcgatgggcg gcgccggcgt tggtccggtg ggcgccagca
     8281 gcacgtcgaa gcgcgccagc gcaccctcga gctgcgcgcg gatggccgcg cgggcccgcg
     8341 cggcctgatg cgcgagggtg gcgggtagca gcgccgcggt cacgagccgc cgccgcgtgc
     8401 cgggatcgta ctcgctgccg cgggtggcca gggtacggtg atgccaggcc gccgcctccc
     8461 cgtcggccag ggccatgaac accgcgccgg ccagcgggag cagcggcagg tcgacgtcct
     8521 ccacccgcgc gctgaggcgg cggagcgtct cggcggcctc gcgaaccgcg gtgcggtcgg
     8581 aggcgtcggt gtccgggctg tccaggagct gggtgacgac gccgatcg
//
LOCUS       DASUFA010000002         5140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1490125, whole genome shotgun sequence.
ACCESSION   DASUFA010000002 DASUFA010000000
VERSION     DASUFA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490125"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VFX87_00050"
     CDS             complement(<1..1106)
                     /locus_tag="VFX87_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4993318.1"
                     /translation="MLRELSIRNLAVVEEAVVPFAAGLNVLTGETGAGKSIVVDALLL
                     ITGARAQLDWIRTGADTAVVSAVFEIDPAGPVTAVLDEAGHRAGDGELVIKRELARTG
                     RHRAFVNDAPATVGLLERLGELLVELHGQHEHQRLMEPARQLLLLDRFAGAEERRDRL
                     GALVRRWEEARAALRLLREEMREGARQEDLYRFQLSEIDGVQLKDGEEDELRAERGRL
                     QHAERIAAGLQEALGLLYEDPQSAAARLARAAALLRDVSRHEPGAAAPIEAIEGAQAY
                     VEDVVGRARALRDRAVFDPERLEQMDARLDAIVKLKRKYSDSVAAILAHRHEVAAALD
                     RITRHDAIAEDMERAVAEAAASAGAEAAALSEART"
     gene            complement(1154..2011)
                     /locus_tag="VFX87_00055"
     CDS             complement(1154..2011)
                     /locus_tag="VFX87_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX4993319.1"
                     /translation="MKRLGIVAKTDREEARTVVPQLLEWCAAHGIQPVVEKETAALCP
                     EATVPMAGRPELPAQSDLLLVLGGDGTLLSMARLVGDLGVPILGVNLGGLGFLTALTL
                     DELFPALEAYLRGELVIEERMLLEARVFRQGERLGEYSALNDVVITKSAMSRIIRLEV
                     AVDGVFATGYRADGLILSTPTGSTAYCLSAGGPIVFPTMDAVVLTPICSHTLTNRPIV
                     LPATQRISITLLTDQDVMLTLDGQVGFALKVHDVVEVRRATARTQLLRVPQKNFFSVL
                     RTKLKWGER"
     gene            complement(2008..2925)
                     /locus_tag="VFX87_00060"
     CDS             complement(2008..2925)
                     /locus_tag="VFX87_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX4993320.1"
                     /translation="MKRSPKKRGLSKPLPPRKTAAAVRGKTVAGTERAGGETRAKTKV
                     RLDIALVERRLLESREKAARVILAGDVIVDGQRVDKAGALVAPGARIELAARPRFVSR
                     GGDKLAHALVAFAIEPRGRVAIDVGASTGGFTHCLLEQGAVKVYAVDVGQGQLDAKLR
                     ADGRVVVMEKTNARSLPIDSFPESPDLATVDISFISLEKVLPSVFNVLTSKGEAVVLV
                     KPQFEVGKGQVGKGGVVRDSSHHRAVLARVARFAVLHGWHVRGVTASPLKGPKGNREF
                     FLLLTKTGRTLAELDALITQTTAEEVPVP"
     gene            complement(3119..3814)
                     /locus_tag="VFX87_00065"
     CDS             complement(3119..3814)
                     /locus_tag="VFX87_00065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX4993321.1"
                     /translation="MFPLKDDNPSRSTPIVTIGLILANALVFLYQVSLEVGDGQGARA
                     GQAFIEEFGLVPCRLTGACRAAMDLPSPILTIFSSMFMHGGLFHIAGNMLYLWIFGNN
                     VEDVLGHGRYLIFYLASGLAAALAQTAIGPSSEVPMVGASGAVSGVLGAYLVLFPHAH
                     VTTLIVLGFFFRLVKIPAVIVLGFWIVVQVLNGLGSFGASGGVAFFAHIGGFLAGMGL
                     LFVLRPRAGTRAT"
     gene            complement(3821..>5140)
                     /locus_tag="VFX87_00070"
     CDS             complement(3821..>5140)
                     /locus_tag="VFX87_00070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4993322.1"
                     /translation="AAGTRAVSPQRAAALALGVALGWAGAGSAAAQPARQQLPGGLTV
                     LVRENTATAVTAASLFVRMGSRWETEDDAGISHLLQQVLLKGTEKRSALEIAETAENL
                     GGGIGASAGLDYSEIGATALARNWRTMLELIADVALRPTLPTAEIDGERRATLTALRS
                     RQDQPSSLAMDTLMSRVYGDHPYGRPALGRPAALERIDRARLLAHHQRFYRAPRMILA
                     MSGDVDTRLVMAEVARLFADAPTGPAEAESTLPTAAARADRTVIVRSAAQAQVLAGFL
                     APPTSHADYAAVKVLATALGGGMAGRLFTEVRDKQGLAYFTGSAYASREGPGVLFTQL
                     GTAPANQARAEAAMLGELERIRREPVSPAELARAKGYELGRFGLDRRTNARLSWYDAF
                     FEALGVGPGFAERYVRAVEAVTVEDVQRVARAYLSAPTVVTLGPAAQ"
BASE COUNT          743 a         1890 c         1818 g          689 t
ORIGIN      
        1 gtgcgggcct ccgagagggc cgccgcctcg gcgcccgccg aggccgccgc ctcggccacc
       61 gcgcgctcca tgtcctcggc gatcgcgtcg tggcgcgtga tccgatccag cgccgccgcc
      121 acctcgtgcc ggtgggcgag gatcgcggcc accgagtcgc tgtacttgcg cttgagcttg
      181 acgatggcgt cgagccgcgc gtccatctgc tcgagccgct cgggatcgaa gaccgcgcgg
      241 tcccggagcg cccgcgcccg gccgaccacg tcctcgacgt aggcctgcgc gccctcgatc
      301 gcctcgatgg gcgcggcggc cccgggctcg tggcgcgaga cgtcgcggag cagcgcggcc
      361 gcgcgcgcca gccgggccgc cgccgactgg gggtcttcat agagcagccc gagcgcctcc
      421 tgcaggcccg ccgcgatccg ctcggcgtgc tggagccgcc cacgctcggc ccggagctcg
      481 tcctcctcgc cgtccttgag ctggacgccg tcgatctcgg agagctggaa gcggtagaga
      541 tcttcctggc gggcgccctc gcgcatctcc tcgcgcagaa gccggagcgc cgcacgcgcc
      601 tcctcccacc ggcgcaccag cgcgcccagc cggtcgcggc gctcctccgc ccccgcgaag
      661 cggtccagca gcaggagctg ccgcgccggc tccatcaggc gctggtgctc gtgctggccg
      721 tgaagctcca ccaggagctc gcccagccgc tcgagcagcc ccacggtggc gggcgcgtcg
      781 ttcacgaagg cgcggtgccg gccagtgcgc gccagctcgc gcttgatgac gagctcgccg
      841 tcgcccgccc ggtgcccggc ttcgtcgagc accgcggtca ccgggccggc cgggtcgatc
      901 tcgaacaccg cggagaccac cgcggtgtcg gcgccggtcc ggatccagtc gagctgggcg
      961 cgggcgccgg tgatgagcag gagggcgtcc accacgatgg atttgccggc gccggtctcg
     1021 ccggtgagca cgttgagacc ggcggcgaac ggcaccaccg cctcttccac caccgcgaga
     1081 ttgcgaatcg acagctcgcg cagcatcgtc ccggccatgc tacggccaac gccgcggggc
     1141 gctcaactgt atcctatcgc tcgccccact tgagcttcgt gcggagcacc gagaagaagt
     1201 tcttctgggg cacgcggagg agctgggtcc gggcggtggc ccggcgcacc tcgacgacgt
     1261 cgtgcacctt gagcgcgaag cccacctggc cgtccagggt cagcatgacg tcctggtcgg
     1321 tgagcagggt gatcgagatg cgctgggtgg cgggcagcac gatcggccgg ttggtgaggg
     1381 tgtgcgagca gatcggggtg agcaccaccg cgtccatcgt cgggaacacg atggggccgc
     1441 ccgccgacag gcagtaggcg gtcgatccgg tgggggtgga gaggatgagc ccgtccgcgc
     1501 ggtagccggt ggcgaagacc ccgtccaccg ccacctcgag ccggatgatg cggctcatgg
     1561 ccgacttggt gatgaccaca tcgttgagcg cggagtactc gccgagccgc tccccctgcc
     1621 ggaacacgcg ggcctccagc agcatgcgct cctcgatgac gagctccccg cgcaggtagg
     1681 cctcgagcgc cgggaacagc tcgtccagcg tgagcgcggt gaggaagccg aggccgccga
     1741 ggttgacgcc caggattggc acgcccaggt cgcccaccag gcgcgccatg ctgagcaggg
     1801 tgccgtcacc gcccaggacg aggaggagat cggactgggc cggcagctcc ggccgacccg
     1861 ccatcggcac cgtcgcctcc gggcagagcg ccgccgtctc cttctcgacc accggctgga
     1921 tcccgtgggc cgcgcaccac tccagcagct gcggcaccac cgtgcgggct tcctcccggt
     1981 cggtcttggc gacgataccc agccgcttca cggcaccggc acctcctccg cggtggtctg
     2041 ggtgatcagc gcgtccagct cggcgagcgt gcggccggtc ttggtcagga ggaggaagaa
     2101 ctcgcggttg cccttcgggc ccttgagcgg cgacgcagtg acgccgcgca cgtgccagcc
     2161 gtgcagcacc gcgaagcggg ccacccgggc cagcaccgcc cggtgatgcg agctgtcccg
     2221 gaccacgccg cccttgccca cctgcccctt gccgacctcg aactgcggct tcaccagcac
     2281 gaccgcctcg cccttcgagg tgagcacgtt gaacacggac gggagcacct tctccagcga
     2341 gatgaatgag atatccacgg tggccagatc gggcgactcg gggaaggaat cgatcggcag
     2401 gctgcgcgcg ttggtcttct ccatcaccac cacccgcccg tcggcgcgca gcttcgcgtc
     2461 gagctggccc tgccccacgt ccaccgcgta gaccttcacc gcgccctgct ccagcaggca
     2521 gtgggtgaag ccgccggtgg aagcgcccac gtcgatggcc acacgccccc tgggctcgat
     2581 cgcgaaggcg acgagcgcgt gcgccagctt gtcgccgccg cggctcacga agcgcgggcg
     2641 cgccgccagc tcgatgcgcg cgccgggtgc gacgagcgcg cccgccttgt ccacccgctg
     2701 cccgtcgacg atcacgtcgc cggccaggat cacccgcgcc gccttctcgc gcgattcgag
     2761 gagccggcgc tcgaccagcg cgatgtccag acggactttt gtttttgcgc gagtttcgcc
     2821 gcctgccctc tcggttccgg cgaccgtctt cccgcggaca gcggcggcgg tcttccgtgg
     2881 cgggaggggc ttcgacaggc cacgcttctt cggggacctc ttcacgccgg ctcctttctt
     2941 gagccgcccg cgcctccggc caggagatgg cagggcggac gatcgcagag aaagacggtg
     3001 gaccgctgct ggtgagggaa cagctcgacc gcgttcatgc gggctcctaa gccccagaga
     3061 ccgtgccaga aatcacaacg gtcacaatta tatagccgaa ccccctccgg gaacgaattc
     3121 aggtggcgcg ggtgcccgcc cggggcctca gcacgaacag cagccccatg ccggccagga
     3181 agcctccgat atgggcgaaa aaggccaccc ccccgctcgc cccgaagctg ccgagcccgt
     3241 tgagtacctg gaccacgatc cagaagccga gcacgatcac ggcggggatc ttcacgaggc
     3301 ggaagaagaa gcccaggacg atcagcgtgg ttacgtgcgc atgcgggaag agcaccaggt
     3361 aggcgccgag cacgcccgag accgcgccgc tggcgcccac catggggacc tccgagctcg
     3421 ggccgatggc ggtctgggcc agcgcggcgg ccaggcccga cgcgaggtag aagatcaggt
     3481 agcggccgtg gcccaggacg tcctcgacat tgttcccgaa gatccacaag tagagcatgt
     3541 tgcccgcgat gtggaagagc ccgccgtgca tgaacatcga gctgaagatc gtcaggatcg
     3601 gcgacggcag gtccatcgcc gcacggcagg cgccggtgag ccggcagggc accagcccga
     3661 actcctcgat gaaggcctgc ccggcccgcg cgccctgccc gtctccgacc tccagcgaga
     3721 cctggtagag gaagaccagc gcgttggcca ggatcaggcc gatggtgacg atcggggtgc
     3781 tccgggaggg gttgtcgtcc ttgaggggga acacgagtcc ctactgcgcc gcggggccga
     3841 gcgtgaccac ggtgggcgcg ctcagatagg ctcgcgcgac ccgctgcacg tcctccaccg
     3901 tcaccgcctc caccgcccgc acgtagcgct cggcgaatcc gggcccgacc ccgagcgcct
     3961 cgaagaaggc gtcataccag gacagccgcg cgttggtgcg ccggtccagc ccgaatcgcc
     4021 cgagctcgta ccccttggcg cgcgccagct cggccgggct cacgggctcg cggcgaatgc
     4081 gctcgagctc cccgagcatc gcggcctccg cccgggcctg gttggcgggc gcggtcccga
     4141 gctgcgtgaa gagcacgccc gggccctcgc gcgacgcata ggcgctgccg gtaaagtaag
     4201 ctaggccctg cttgtcgcgc acctcggtga agagacgccc ggccatgcca ccacccagcg
     4261 cggtggccag caccttcacc gccgcgtagt cggcgtgcga cgtgggcggg gcgaggaagc
     4321 cggccaggac ctgcgcctgc gccgccgacc gcacgatcac ggtgcggtcc gcgcgcgccg
     4381 ccgccgtggg cagcgtggac tcggcctccg cggggccggt gggagcgtcg gcgaagaggc
     4441 gcgccacctc cgccatcacc aggcgcgtgt ccacgtcgcc gctcatcgcc aggatcatgc
     4501 gcggggcccg atagaagcgc tggtggtggg cgagcagccg ggcgcgatcg atgcgctcca
     4561 gcgcggcggg ccggcccagg gccgggcggc cgtaggggtg atccccgtag acgcggctca
     4621 tgagcgtatc cattgccagg gaggacggct ggtcctggcg gctgcggagc gcggtcagcg
     4681 tggcgcggcg ctcgccgtcg atctcggcgg tgggcagcgt gggccggagc gcgacgtcgg
     4741 cgatcagctc gagcatcgtg cgccagtttc gcgcgagcgc ggtggcgccg atctcggagt
     4801 agtcgagacc ggccgaggcg ccgatgccgc cgcccaggtt ctccgcggtc tcggcgatct
     4861 cgagcgccga gcgcttctcg gtccccttga gcagcacctg ctggagcaga tggctgatac
     4921 ccgcgtcgtc ctcggtttcc cagcgcgagc ccatccgcac gaagagcgag gctgccgtca
     4981 ccgcggtcgc ggtattctcc cgcaccagca cggtgaggcc gccggggagc tgctggcgcg
     5041 ccggctgggc cgccgcgctc cccgccccgg cccacccgag ggccacgccg agcgccaggg
     5101 ccgccgcgcg ctgcgggctc actgcgcgcg tccccgcggc
//
LOCUS       DASUFA010000003         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1032310, whole genome shotgun sequence.
ACCESSION   DASUFA010000003 DASUFA010000000
VERSION     DASUFA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032310"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFX87_00075"
     CDS             complement(<1..1197)
                     /locus_tag="VFX87_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX4993323.1"
                     /translation="MARDLTELTRIIRRIEAKNRVRPPAGSPRSLEALLDGAVEETAR
                     GKLLVVRRRFPVGHRHGAQPLLAAREATPAALALLSRGGLPVSEGRRLLYLDTETTGL
                     AGGTGTYAFLVGVGFFDGDEFEVRQFFMRDLDEEPALLTVLEETFRKFDGFVTYNGAG
                     FDLPLLETRFVLGRRRFPGDVFHVDLLGPARRLWSARLADCRLGTVEQDVLGFLRDDD
                     LPGALIPTVYFEYLRRKRPDELPRVFEHNRHDILSLAALTGWVADAVARAPHPELDPE
                     ALAGLGRMLEAADPERSLACYRLALDTGLPTPSRERLLLRLAHREKRLARWDEARALW
                     EAAALSPREFDPRPWEEIAKVHEHRRRDFTAARVVVEQALDLARRHRASERVLAAFEH
                     RLTRLAR"
     gene            complement(1211..>3109)
                     /locus_tag="VFX87_00080"
     CDS             complement(1211..>3109)
                     /locus_tag="VFX87_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX4993324.1"
                     /translation="NLVLTNPDMLHSGILPHHTKWVNLFQNLKYVVIDELHAYRGVFG
                     SHLANVLRRLKRICRHYGATPQFIMASATIANPGDLAQRLTGETVVELNESGAPTGEK
                     TFMVYNPPVVNPELGIRAPYLGEASKLATRFLKQKVATIVFCQSRLSTEVVLASIKKG
                     VEDKTGDSGIVRGYRGGYLPLRRREVEQGLRSGDVLGVVSTSALELGIDIGHLDVAVL
                     AGYPGTIASMWQQAGRAGRRSGESAAVMVATSSPMDQYLAAHPDYLFGASPEHARINP
                     ENPFILINHVKCAAFELPIGAEEAFGGDVSAHLAALEDEGVLHRAGEHFHWSSETYPA
                     DHISLRTVTSDNFLVIDTTARDAAQVKRRQIIAEVDWKSAFSMIYPKAIYMLEGDPFE
                     VQELHYREDEEKVAYVKRVAVDYFTDAISAKGVWILQRFGRQETPGLLAEQGEVLVAE
                     KVVGFKKIKMGTLENVGSGEVELPQQEMQTTSVWLTLHPELLAEISPRRDDLVDGLRA
                     LTHLLHSLAPIFLLCDIRDVGAWLGDGSPSQAGQVVTREIMRAQLLQGETFTPTIYLY
                     DNQPGGIGLAERIFEVLPELLARGLETLEACGCRSGCPSCVGPVNEVGRQAKPVALGI
                     LKRLVPRR"
BASE COUNT          456 a         1100 c         1099 g          454 t
ORIGIN      
        1 gcgggccagc cgcgtgagcc ggtgctcgaa ggcggcgagc acccgttcgg acgcccgatg
       61 gcgccgggcc agatcgaggg cctgctccac caccacccgc gcggcggtga agtcgcgccg
      121 gcgatgctcg tggaccttgg cgatctcctc ccacggtcgc gggtcgaatt cgcggggcga
      181 gagggccgcc gcctcccaga gcgcgcgcgc ctcgtcccag cgcgccaggc gcttctcgcg
      241 gtgggcgagc cgcagcagca ggcgctcgcg cgagggtgtc ggcaggccgg tgtcgagcgc
      301 caggcggtag caggccaggc tccgctccgg gtccgcggcc tcgagcatcc ggccgaggcc
      361 ggccagcgct tccggatcga gctcgggatg cggcgcgcgc gccaccgcat ccgcgaccca
      421 gccggtgagc gcggccagcg agaggatgtc gtggcggttg tgctcgaaca cccgcggcag
      481 ctcgtccggc cgcttccgcc gcaggtactc gaagtagacg gtggggatca gcgcgcccgg
      541 caggtcgtcg tctctcagga agccgagcac gtcctgctcg accgtcccga gccggcagtc
      601 ggccaggcgc gcgctccaga gccgtcgcgc cggaccgagc aggtccacgt ggaagacgtc
      661 cccggggaag cggcgccggc cgagcacgaa gcgcgtctcg agcagcggca ggtcgaagcc
      721 ggccccgttg taggtcacga agccgtcgaa cttccggaac gtctcctcga ggacggttag
      781 cagcgccggc tcctcgtcga ggtctcgcat gaagaactgg cgcacctcga actcgtcgcc
      841 gtcgaagaag ccgacgccga ccaggaacgc gtacgtgccg gtgccgcccg ccagcccggt
      901 ggtctcggtg tcgagataga gcaggcggcg accctcgctc accggtaggc cgccgcggct
      961 cagcagggcg agcgcggccg gcgtcgcctc acgcgcggcc agcagcggct gcgccccgtg
     1021 ccggtgcccc accgggaacc ggcggcgcac caccagcagc ttcccgcgcg cggtctcctc
     1081 gacggccccg tcgagcaagg cttccagaga tcggggtgag cccgcgggcg ggcgcacacg
     1141 gttcttcgcc tcgatgcggc ggatgatgcg ggtcagctcg gtgagatcgc gcgccacggg
     1201 agcggaggcg tcagcgccgg ggcacgaggc gcttgaggat cccgagcgcg accggcttgg
     1261 cctggcgccc cacctcgttc acgggcccca cgcacgaagg gcagccgctc cggcagccgc
     1321 aggcctcgag cgtctcgagc ccgcgcgcca gcagctccgg cagcacctcg aagatccgct
     1381 cggccaggcc gatgccgccc ggctggttgt cgtagaggta gatcgtcggc gtgaaggtct
     1441 caccctggag gagctgggcc cgcatgatct cgcgcgtcac cacctgcccc gcctgggacg
     1501 gcgagccgtc gccgagccac gcccccacgt cgcggatgtc gcacagcagg aagatcgggg
     1561 ccaggctgtg gaggagatgg gtgagggcgc ggagcccgtc caccagatcg tcgcgccgcg
     1621 gggagatctc ggcgagcagc tcgggatgga gcgtgagcca gacgctggtg gtctgcatct
     1681 cctgctgcgg cagctccacc tcacccgagc ccacgttctc cagcgtaccc atcttgatct
     1741 tcttgaagcc gacgaccttc tcggccacca gcacctcgcc ctgctcggcc agcagccccg
     1801 gggtctcctg ccgcccgaaa cgctggagga tccacacgcc cttggcgctg atcgcgtcgg
     1861 tgaagtagtc caccgccacc cgcttgacgt aggccacctt ctcctcgtcc tcgcggtagt
     1921 ggagctcctg cacctcgaat gggtcgccct ccagcatgta gatcgccttc gggtagatca
     1981 tggagaaggc gctcttccag tccacctccg cgatgatctg cctccgcttg acctgggccg
     2041 cgtcgcgcgc ggtggtgtcg atcaccagga agttgtcgga ggtgacggtg cgcagcgaga
     2101 tgtggtcggc cggataggtc tccgagctcc agtggaagtg ctcgccggcc cggtggagca
     2161 cgccctcgtc ctcgagtgcg gccaggtgtg ccgacacgtc gccgccgaag gcctcctccg
     2221 ctccgatcgg cagctcgaag gccgcgcact tgacgtggtt gatcaggatg aacggattct
     2281 ccggattgat ccgcgcgtgc tcgggcgacg ccccgaagag gtagtcggga tgcgcggcca
     2341 gatactggtc catcggagag ctggtggcca ccatcaccgc ggcggactcc ccgctgcgcc
     2401 ggcccgcgcg tcccgcctgc tgccacatgg aggcgatggt gccgggatag cccgcgagca
     2461 cggcgacgtc gaggtgcccg atgtcgatcc ccagctcgag cgcgctcgtg gacaccaccc
     2521 ccagcacgtc gcccgagcgg aggccctgct ccacctcgcg ccgccgcagc ggcaggtagc
     2581 cgccgcggta gccgcgcacg atgccggagt cgcccgtctt gtcctcgacg cccttcttga
     2641 tcgaggccag cacgacctcg gtcgacagcc gactctggca gaacacgatg gtggcgacct
     2701 tctgcttcag gaaacgggtg gcgagcttgg acgcctcacc gaggtagggc gcgcggatgc
     2761 cgagctcggg attcaccacc ggcgggttgt agaccatgaa ggtcttctcg ccggtgggcg
     2821 ccccgctctc gttcagctcg accaccgtct cgccggtgag ccgctgggcc aggtcgcccg
     2881 gattggcgat ggtggccgag gccatgatga actgcggggt cgccccgtag tgccggcaga
     2941 tgcgcttgag ccgccgcagc acgttcgcga gatggctgcc gaagacgccc cggtaggcgt
     3001 gcagctcgtc gatcacgacg tacttgaggt tctggaagag attcacccac ttggtgtggt
     3061 gcggcaggat cccggagtgc agcatgtcgg gattggtcag caccaggtt
//
LOCUS       DASUFA010000004         3723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_229752, whole genome shotgun sequence.
ACCESSION   DASUFA010000004 DASUFA010000000
VERSION     DASUFA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229752"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VFX87_00085"
     CDS             <1..1303
                     /locus_tag="VFX87_00085"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX4993325.1"
                     /translation="VEVYPRGVQSKVRGLQVHGHAVPQATAGQRTAVNLQGVERAAIE
                     RGDVLAPAGTLVPTLLLDGTLELLEDAPRPVKTRDRVRFHVGTQEVMARVLPVDRSEL
                     DPGGSCHARFRLEAPVVALPGDRFVVRSYSPIVTIGGGTVLDIAPPRFKRRGPALAAH
                     LALLATGEPSAVLEEHLKQAGAAGARVADLRARTPFGPSLLRELLDQLQHDGRVLAVD
                     REWYLHRDASERLRSQTLGLLEAFHAENPLRGGISREELRSRAGNAQERVFTQLLTAL
                     ETEGAVKSERDQVRLGSHAIRLSPDQERVVKGLEADFRAAGAAPPSPEEALGRLGVKG
                     TEKHELFQVLVAGRTLLRVKESLFFHAEALRTVQEQLVALLRQKKEIGPADFKDLFGV
                     SRKYAIPLMEYFDTQRVTVRVGERRVLREGYGRVDNPGAGA"
     gene            1341..1529
                     /gene="tatA"
                     /locus_tag="VFX87_00090"
     CDS             1341..1529
                     /gene="tatA"
                     /locus_tag="VFX87_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX4993326.1"
                     /translation="MFGLGYQELLLILVIVLILFGAQRLPDLARSLGGSIKEFKKGVA
                     EVKDEPATAVKKEDEKKA"
     gene            1588..3198
                     /locus_tag="VFX87_00095"
     CDS             1588..3198
                     /locus_tag="VFX87_00095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4993327.1"
                     /translation="MARHGDLCLALGLAVVTLLSRWPYRARMLYNWDAVQFALALREF
                     DVAKHQPHPPGYFLYVGLGRLLNASLGDPTLAYVALAMLFSASSTFVLYALARRIYDR
                     ATALVAASLLAVSPLFWFYGSVGLTYAGEAFAASLVAWFAYATLAGSARHLYWGALAL
                     GLTGGMRQSVLLLLLPLWLGCALLGVRSRRRLALAGGILVGSVLAWLVPMVWLSGGPA
                     AYLAASTQLYGSVLFPTSVLGGSLEITLAQVRYLLESTLVGLGPLGLVALALPVYARR
                     VGWRTPELFLLAWMVPAGIFYTLVHFGQAGYVLTFLPALVILLSRVLVWAVAAGSERL
                     RRPNWRWALTAATVLPLVLINTGFFVSARPLPREFEHRPGGDTWLWRARDEFHDWIMS
                     RTAAALREHEAVIRTYVETIRTVYEPSDTALVTELGNPRSYPWLRHAMFYMPEYPIYQ
                     VQVGPMPPGFYAPQSSATMILTPGSHIRLPAETRRLVWFVDHWDPATPRPDGLREVQL
                     PYGRYLYVLPLTRKPAEYAGYTFERAAR"
     gene            3195..>3723
                     /locus_tag="VFX87_00100"
     CDS             3195..>3723
                     /locus_tag="VFX87_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993328.1"
                     /translation="MRGRTLAVAAPVLALTLAIGFFLLRTRQLAGVLGVEAFPLDDSW
                     IHMQFARNLAEGRGFSYNPGVPVSGSTAPLWTLALGGVFAVFGSHPVLAKALGIAATL
                     GTAWLAGRLALIWVARHDVAILASVLVALAGPMVWGALSGMEVGLAALLATAALMLHA
                     RGRAWAAGTALGLAAL"
BASE COUNT          430 a         1360 c         1314 g          619 t
ORIGIN      
        1 ggtcgaggtg tatccacgag gcgtgcagtc gaaggtgcgc ggcctgcagg tgcacggcca
       61 cgccgtcccg caggccaccg ccggccagcg caccgcggtg aatctccagg gggtggagcg
      121 ggcggccatc gagcgcggcg atgtcctcgc gccggccggc accctcgtgc cgacgctgct
      181 gctcgacggc acgctcgagc tcctggagga cgcgccgcgg cccgtcaaga cgcgcgaccg
      241 cgtccgcttc cacgtgggta cccaggaggt gatggcgcgc gtcctgccgg tggaccggtc
      301 cgagctcgat cccggcggat cctgtcatgc ccgcttccgg ctggaggccc cggtggtggc
      361 gctgccgggg gaccgcttcg tggtgcggtc ctactcgccc atcgtgacca tcggcggcgg
      421 taccgtgctg gacatcgcgc ccccgcgctt caagcgcagg gggcccgcgc tcgccgctca
      481 tctggccctg ctcgccaccg gcgagccctc ggcggtgctg gaggagcacc tgaagcaggc
      541 cggggccgcc ggcgcgcggg tcgccgatct ccgcgcccgg acgcccttcg ggccttcgct
      601 gctccgggag ctcctcgacc agctccagca cgacggccgc gtcctcgcgg tggatcgcga
      661 gtggtacctg catcgcgatg cgagcgagcg cctgcgctcc cagacgctgg gcctcctcga
      721 ggcctttcac gccgagaatc ccctgcgcgg cgggatctcc cgggaggagc tgcgcagccg
      781 ggccggcaac gcccaggagc gggtcttcac ccagctcctg accgcgctgg agacggaggg
      841 ggccgtcaag agcgagcggg accaggtgcg gctcggctct cacgccatcc ggctcagccc
      901 ggaccaggag cgggtggtca aggggctcga ggccgacttc cgcgcggcgg gcgccgcgcc
      961 cccgagcccc gaggaggctc tgggccggct gggtgtcaag ggcacggaga agcacgagct
     1021 cttccaggtc ctggtcgcgg gccggaccct gctgcgcgtg aaggagtcgc tcttcttcca
     1081 cgccgaggcg ctccggaccg tccaggagca gctggtggcc ctcctgcgcc agaagaagga
     1141 gatcggtccc gccgacttca aggacctctt cggggtcagc cggaagtacg ccatcccgct
     1201 gatggagtac ttcgacaccc agcgcgtgac cgtgcgggtc ggggagcgtc gggtgctccg
     1261 ggaaggctac ggtcgcgttg acaaccccgg ggcgggcgcg tagggtaagg gggcgtcagg
     1321 aaaaggaaag gagtcccgcc atgttcggtt tgggatatca agagctgctg ttgatcctgg
     1381 tcatcgtgct gatcctcttc ggggcgcagc gtctccccga tctggcccgt tcgctcggcg
     1441 gcagcatcaa ggagttcaag aagggcgtgg ccgaggtcaa ggacgagccc gcgacggccg
     1501 tgaagaagga agacgagaag aaggcgtagc tccgctcccc tgaagcactt cgtcacccgc
     1561 ctcctcgact tctctcgcat cgcctgcgtg gcgcgccacg gcgacctgtg cctcgcgctg
     1621 ggacttgccg tcgtcacgct gctctcgcgc tggccctatc gcgcccgcat gctctacaac
     1681 tgggacgcgg tccagttcgc tctcgcgctg cgcgagttcg acgtcgccaa gcaccagccg
     1741 catccacccg gctacttcct ctacgtcggg ctgggccgcc tgctcaatgc ctcgctgggc
     1801 gatcccacgc tggcgtacgt ggcgctcgcg atgctcttca gtgccagctc caccttcgtc
     1861 ctctacgcgc tggcgcgccg gatctacgat cgcgccaccg cactcgtggc ggcgtcgctg
     1921 ctcgcggtca gcccgctctt ctggttctac ggctcggtgg gcctcaccta tgccggcgag
     1981 gcgttcgccg cgtcgctggt ggcctggttc gcctacgcga cgctcgccgg cagtgcgcgg
     2041 catctctact ggggcgcgct ggccttaggt ctcaccggcg gcatgcggca gtcggtgctg
     2101 ctcctgctcc tgccgctctg gctcgggtgc gcgctgctgg gtgtccgctc gcggcgccgc
     2161 ctggcgctgg cgggcgggat cctcgtggga tccgtgctcg cctggctcgt gccgatggtg
     2221 tggttgagcg gcggtcccgc cgcctacctg gccgcctcca cgcagctcta cggctcggtg
     2281 ctcttcccga cctcggtgct gggcggctcg ctcgagatca cgctcgccca ggtgcgctac
     2341 ctgctcgagt ccaccctggt cgggctcggc ccgctcggcc tggtggcgct ggcgttgccg
     2401 gtgtacgcgc gccgggtggg ctggcgaacg cccgagctct tcctgctcgc gtggatggtg
     2461 ccggcgggga tcttctacac gctcgtgcac ttcgggcagg cgggctacgt gctgaccttc
     2521 ctgcccgcgc tcgtgatcct gctgagccgg gtgctcgtgt gggcggtcgc ggcgggctcc
     2581 gagcggctgc gccggccgaa ctggcgctgg gcactcaccg ccgccacggt gctgccgctg
     2641 gtgctgatca ataccggctt cttcgtgagc gcgcgtccgc tgccgcgcga gttcgagcac
     2701 cggccggggg gcgatacatg gctctggcgg gcgcgcgacg agttccacga ctggatcatg
     2761 agccgcaccg cggccgcgct gcgcgagcac gaggcggtga tccgcactta cgtcgagacc
     2821 atccggaccg tctacgagcc ctccgacacc gcgctcgtga ccgagctcgg caatccgcgt
     2881 tcctacccgt ggctgcgcca cgcgatgttc tacatgccgg agtacccgat ctaccaggtg
     2941 caggtcggcc cgatgccccc cggcttctac gcgccgcaat cctccgccac catgatcctc
     3001 acgccgggct cgcacatccg gctgcccgcg gagacccgac ggctcgtctg gttcgtggac
     3061 cactgggatc cggcgacgcc gcggcccgac ggcctgcggg aagtccagct cccgtacggc
     3121 cgctatctct acgtcctgcc gctcacccgc aagcccgccg agtacgccgg ctacaccttc
     3181 gagcgcgccg cccggtgagg ggccggacgc tcgccgtcgc cgcccccgtc ctcgccctca
     3241 ccctcgcgat cgggttcttc ctgctgagga cgcgccagct cgcgggtgtg ctcggggtcg
     3301 aggccttccc gctcgacgat tcgtggatcc acatgcagtt cgcgcgcaat ctggccgagg
     3361 ggcgcggctt ctcctacaac ccgggcgtgc cggtgtcggg ctcgaccgcg ccgctctgga
     3421 ccctcgcgct cggcggggtg ttcgcggtgt tcggcagcca tccggtcctg gcgaaggcgc
     3481 tggggatcgc cgccacgctc gggaccgcgt ggctggcggg ccgcctcgcc ctgatctggg
     3541 tcgcgcgtca cgacgtcgcc atcctcgcct ccgtgctcgt ggccctggcc gggccgatgg
     3601 tgtggggcgc gctctccggc atggaggtcg ggctcgccgc gctgctggcg acggccgcgc
     3661 tcatgttgca cgcgcgtggt cgcgcctggg cggccggcac cgcgctcggg cttgccgcgc
     3721 tcg
//
LOCUS       DASUFA010000005         4857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_344500, whole genome shotgun sequence.
ACCESSION   DASUFA010000005 DASUFA010000000
VERSION     DASUFA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344500"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VFX87_00105"
     CDS             <1..159
                     /locus_tag="VFX87_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEX4993329.1"
                     /translation="GMHPGQLKDMVSSPGGTTIAGIAALEEGGVRRTFISAVERATQR
                     SRELGKRS"
     gene            161..424
                     /locus_tag="VFX87_00110"
     CDS             161..424
                     /locus_tag="VFX87_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX4993330.1"
                     /translation="MLDLIFLLLDIYKWVIIAAALITWVSPDPRNPIVMILRQITEPV
                     LAPARRLLPPWKTGGLDFSPLIVIFAIQLVEWALPRLLFQAQH"
     gene            435..911
                     /locus_tag="VFX87_00115"
     CDS             435..911
                     /locus_tag="VFX87_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX4993331.1"
                     /translation="MRITPMDIRQQQFTVRMFRGFDVQEVDTFLEDLAGDYEAMLKEN
                     TLLKEHLQAAEERTRGLEQRERMLQETLVTTQRMVEEMKDNARREAQLVIKEAEMQGE
                     KTIEAARTAEGAIQAQISQLKRTRRQLAESLRATVDMYQRLLDQDLEADANQGDGG"
     gene            1018..1284
                     /locus_tag="VFX87_00120"
     CDS             1018..1284
                     /locus_tag="VFX87_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010138568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX4993332.1"
                     /translation="MVQVRVQPKARRNEIVEQPDGVFRVRVTAAPEGGEANRAVTALL
                     AEAFGVAPSRISLVRGATSRDKLFRIEGRPLTLPSPRRREGDRK"
     gene            1281..2297
                     /gene="mtnA"
                     /locus_tag="VFX87_00125"
     CDS             1281..2297
                     /gene="mtnA"
                     /locus_tag="VFX87_00125"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX4993333.1"
                     /translation="MITPVRWEDGGLVLLDQTKLPTEEVERACTAWPDVAAAIRTLVV
                     RGAPAIGVAAAFGVVLAARASGATNQAALLADVEEAIKGLAATRPTAVNLFWALDRMR
                     RLVVAGVDVPPARLRERLLEEAQAILSEDLAANRAMGAHGAALVPDGARLLTHCNAGA
                     LATAGYGTALGVIRAAHERGKLAMVWVDETRPVMQGSRLTAWELAKEGIPHRLISDVA
                     AGFVMKRGEVDLVVTGADRIAANGDTANKIGTYSVAVLARHHGIPFYVAAPSSTIDPS
                     ISSGDSIVIEERDGAEVRGVAGRQTAPAASPVFNPAFDVTPAALISAIITERGVFRPP
                     YHFS"
     gene            2408..3892
                     /locus_tag="VFX87_00130"
     CDS             2408..3892
                     /locus_tag="VFX87_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX4993334.1"
                     /translation="MLDWESKPLPFKVYPALEQIRLPTALPPLAVDTFAALAPGAPSA
                     RPAPLDLERLAALLFFSAGVTRVKTYPGGAQVHFRAAASTGALYQTEVYVIAGAVEGL
                     EPGVYHFSPGDFSLRRLRAGDFRGAVALAAADDAVAAQPASLILSAIYWRNTWKYQAR
                     GYRHLFWDSGTLLGQLLAVARALDVPARLVTGFVDAQLNGLLGLDAEKEGVLVAAPLG
                     APGRVAAAPPVITTLAPEVVPLSSREVDYPVLRDAYDNASLDSEAEVADWRERGGPSR
                     SDVVMHDLTPLPAPLGVAGRSLAETIAKRGSTREFSGEAITAEALSSALSHATGGFPA
                     DVPSGLVDLYVNVHAVTGIEPGAYAYDRRAHALARLRAGDVREASAFLCLEQALGGAS
                     SATVFFLADLARVLERWGNRGYRLVNLEAGLIGGRLYLGAYGQRFGASGLTFYDRAVV
                     EFFSPHAAGKDAIFVTALGRSAKSPLPSRERVAWRAEARGKPSE"
     gene            complement(3898..4392)
                     /locus_tag="VFX87_00135"
     CDS             complement(3898..4392)
                     /locus_tag="VFX87_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993335.1"
                     /translation="MHPPEGAAWLVWLETSALAMAMRQWQWLYPVVEILHILGFVLVV
                     GGAFFFDLRLLGRGRGLAVSGLAAHLLTWARAGFLLVLPTGFMMFTAHATEFAGNPAF
                     RFKLILIASALLNAAAFHRWPFRSVIRWDVGLGTPAWARLAAVLSLVCWIGAIACGRL
                     LAYL"
     gene            complement(4396..4719)
                     /locus_tag="VFX87_00140"
     CDS             complement(4396..4719)
                     /locus_tag="VFX87_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX4993336.1"
                     /translation="MLLALVTAPAAAHHGWSGYDSTKETTLTGTIREAGYEHPHGYIR
                     LEAGGKVWVVVLAPPTRMENRGLPRADLRPGATATVVGYPNRTDPGELRAERITVGGK
                     TTELR"
BASE COUNT          685 a         1755 c         1727 g          690 t
ORIGIN      
        1 ggaatgcatc cggggcagct caaggacatg gtgtcttccc ccgggggtac caccatcgcc
       61 ggcatcgcgg cgctggaaga agggggagtg cggcggacat tcatcagcgc cgtggagcgc
      121 gcgacgcagc gctctcgcga gctggggaag cgatcgtagc gtgctcgatc tgatctttct
      181 gctgctcgac atctacaagt gggtgatcat cgcggccgcg ctgatcacct gggtgagtcc
      241 ggacccgcgc aatcccatcg tgatgattct ccgccagatc accgagccgg tgttggcccc
      301 ggcccggcgg ctgctgccac cgtggaagac cggcgggctc gacttctctc cgctgatcgt
      361 catctttgcc atccagctgg tcgagtgggc gcttccgcgc ctgctcttcc aggctcagca
      421 ctgaggaggc cgccgtgcgc atcaccccca tggatatccg ccagcagcag ttcacggtgc
      481 gaatgttccg cggcttcgac gtccaggaag tggacacgtt cctcgaggac ctggcgggtg
      541 actacgaagc catgctgaag gagaacaccc tgctcaagga gcatcttcag gcggccgagg
      601 agcgcacccg cggtctcgag cagcgcgagc gcatgctcca ggagaccctc gtcaccaccc
      661 agcggatggt cgaggagatg aaggacaatg cccggcgcga ggcccagctc gtgatcaagg
      721 aagccgagat gcagggcgag aagaccatcg aggccgcgcg caccgccgag ggcgccatcc
      781 aggctcagat cagccagctc aagcgcaccc ggcggcagct cgccgagtcc ctgcgggcca
      841 ccgtggacat gtaccagcgc ctgctcgacc aggacctcga ggcggatgcg aaccagggcg
      901 acggcgggta gggcgctcgg aggggggctg cgcccgcctt ccgaacctct ccccaatacc
      961 ggattgcgcg ggctaagccc gcgctcgaac cggaccgcac caccggccag ctcgcagttg
     1021 gtgcaggtgc gcgtccagcc gaaggcgcgg cgcaatgaga tcgtggagca gccggacggg
     1081 gtgttccgcg ttcgcgtgac cgcggccccc gaaggtggcg aggcgaaccg ggcggtgacc
     1141 gcgctcctgg cggaggcatt cggggtcgcg ccgtcccgca tctcgctggt gcgcggggcc
     1201 acctcgcgcg acaagctgtt ccgcatcgag ggacggcccc tcaccctgcc ctctccccgg
     1261 aggagagagg gagaccggaa gtgatcacgc cggttcgctg ggaagacggc gggctcgtgc
     1321 tgctcgacca gacgaagctg cccaccgagg aggtggagcg cgcctgcacg gcctggccgg
     1381 acgtggccgc ggccatccgc acgctggtgg tccgcggggc gcccgccatc ggggtcgccg
     1441 ccgcttttgg cgtcgtcctc gccgcgcggg cgagcggcgc cacgaatcag gccgccctgc
     1501 tcgcggatgt cgaggaggcc atcaagggac tcgccgcgac ccgtcccacc gcggtcaacc
     1561 tgttctgggc gctcgaccgc atgcggcgtc tggtcgtggc cggggtggac gtgccgcccg
     1621 cgcggttgcg tgagcggctc ctcgaggagg cgcaggccat cctgtccgag gacctcgccg
     1681 cgaaccgcgc catgggcgcg cacggggccg cgctggtgcc ggacggggcg cgcctgctca
     1741 cccactgcaa cgcgggcgcg ctggccaccg cgggctacgg caccgcgctc ggggtgatcc
     1801 gggccgcgca cgagcggggc aagctcgcga tggtctgggt ggacgagacg cgcccggtca
     1861 tgcagggctc gcggctcacc gcgtgggagc tggccaagga gggcatcccg caccggctga
     1921 tctcggacgt cgcggcgggc ttcgtgatga agcgcggcga ggtggatctg gtggtgaccg
     1981 gcgcggaccg catcgcggcc aacggcgaca ccgccaacaa gatcggcacg tactcggtgg
     2041 cggtgctggc gcgtcaccac ggcatcccgt tctacgtggc cgcgccgtcc tccaccatcg
     2101 acccctcgat ctcgagcggc gacagcatcg tgatcgagga gcgcgacggg gccgaggtgc
     2161 gcggcgtggc cggtcgccag accgctcccg cggcctctcc cgtgttcaac ccggccttcg
     2221 acgtcacgcc cgccgcgctg atcagcgcca tcatcaccga gcgcggcgtg ttccgcccgc
     2281 cctaccactt cagctagatc ccctctcagg ggggcggctt atctccgcca accgcgacat
     2341 cacgagcgcc cgccggtacc acgaccgcac cgcgcactcg cccgagtcgg tgcggcggag
     2401 cggccacgtg ctcgactggg agagcaagcc gctgcccttc aaggtctacc ccgcgctcga
     2461 gcagattcgg ctcccgaccg cgctccctcc gctggccgtc gacacgttcg ccgcgctcgc
     2521 gcccggcgcc ccgtccgcgc gcccggcgcc gctcgacctc gagcgcctgg ccgcgctgct
     2581 cttcttctcc gcaggcgtca cgcgggtcaa gacctacccg ggtggcgccc aggtccactt
     2641 ccgcgccgcc gcgtccaccg gcgcgctcta ccagaccgag gtctacgtga tcgccggcgc
     2701 cgtcgagggg ctcgagcccg gcgtctacca cttcagcccg ggagatttca gcctgaggcg
     2761 gctgcgtgcc ggcgacttcc ggggcgcggt ggcgctggcc gcggccgacg acgcggtggc
     2821 cgcccagccg gcctcgctga tcctctcggc gatctactgg cgcaatacct ggaagtacca
     2881 ggcgcgcggt taccggcacc tcttctggga ttcgggcacg ctgctgggcc agctcctggc
     2941 ggtggcgcgc gctctcgacg tgccggcgcg cctggtcacc ggcttcgtgg acgcgcagct
     3001 caacggcctg ctcggcctcg atgccgagaa ggagggcgtg ctcgtggccg ccccgctggg
     3061 cgccccgggt cgggtcgccg ccgcgccccc ggtcatcacc acgctcgcgc ccgaggtcgt
     3121 cccgctctcc tctcgcgagg tcgactaccc ggtcctgcgc gacgcctacg acaacgcgag
     3181 cctcgattcc gaagccgagg tcgccgactg gcgcgaacgc ggcggcccct cgcgctcgga
     3241 tgtcgtgatg cacgacctga ccccgctgcc ggcgccgctg ggcgtggccg ggcggtcgct
     3301 ggcggagacg atcgcgaagc gggggtcgac gcgcgagttc tcgggggagg cgatcaccgc
     3361 ggaggcgctg tcgagcgcgc tctctcacgc cacgggtggg ttcccggccg acgtgccgtc
     3421 ggggctggtg gacctctatg tcaacgttca cgcggtgacc gggatcgagc cgggggccta
     3481 cgcgtacgac cggcgggcgc acgcgctcgc gcgcctccgg gcaggggacg tgcgcgaggc
     3541 gtccgccttc ctctgtctcg agcaggcgct gggcggagcg tcgagcgcga ccgtcttctt
     3601 cctcgccgac ctggcgcggg tgctcgagcg ctggggcaat cgcggctacc ggctggtgaa
     3661 cctggaggcg ggtctgatcg gcgggcggct ctacctgggc gcctatggcc agcgcttcgg
     3721 ggccagcggc ctcaccttct acgaccgcgc ggtggtggag ttcttctcgc cccacgccgc
     3781 gggcaaggac gcgatcttcg tcaccgccct cggccgctcc gccaagtctc ctctcccctc
     3841 acgggagcgc gtcgcgtggc gagctgaagc caggggcaag ccgagcgaat agccgtccta
     3901 gaggtacgcg agcaggcgcc cgcacgcgat cgcgccgatc cagcagacga gggacagcac
     3961 cgccgccagc ctggcccagg cgggcgtgcc gagccccacg tcccagcgga tcaccgaccg
     4021 gaacggccac cggtggaagg cggcggcgtt gagcagggcg gaggcgatca ggatcagctt
     4081 gaaccggaac gccgggttcc cggcgaactc ggtggcgtgc gcggtgaaca tcatgaagcc
     4141 ggtgggcagc acgaggagaa agccggctcg cgcccatgtg agcagatgcg cggcgagccc
     4201 gctcaccgca aggccacggc cccgcccgag cagtcgcagg tcgaagaaga aggcgccgcc
     4261 caccacgagc acgaagccca ggatgtgcag gatctcgacg acggggtaga gccactgcca
     4321 ctggcgcatc gccatcgcca gcgcggacgt ctcgagccac acgagccagg cggccccctc
     4381 gggtggatgc acggcctagc ggagttccgt cgtcttcccg ccgaccgtga tccgctcggc
     4441 gcgcagctcg ccgggatccg tacgattggg gtagcccacc acggtggcgg tcgcgccggg
     4501 ccggagatcg gcgcggggca ggccgcggtt ctccatccgc gtgggcggag ccagcaccac
     4561 gacccagacc ttgccgccgg cctcgagccg gatgtatccg tgcgggtgct cgtagccggc
     4621 ctcccggatg gtgccggtga gcgtcgtctc cttggtgctg tcgtagccgc tccagccgtg
     4681 atgggccgcg gcgggggcgg ttacgagcgc caagagcatg agcgtgaacg ggaccgatac
     4741 gtggaatctc accctggcac ctcctccata ttcgctcggc tcgccgtcgg cttcacctcg
     4801 cccggctcgc gcatattcgc tcggctcgcc gtcggcttca cctcgcccgg ctcgcgc
//
LOCUS       DASUFA010000006        11011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1033357, whole genome shotgun sequence.
ACCESSION   DASUFA010000006 DASUFA010000000
VERSION     DASUFA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033357"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFX87_00145"
     CDS             complement(<1..843)
                     /locus_tag="VFX87_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4993337.1"
                     /translation="MTLLTSPADVLAIDTFAAPGDGCLGYLVVDETRRTALAIDPRLD
                     QVDRFVEALATHDARLTFVLDTHTHADHLSGVRRLARRTGATVLAHAASKLLGPVRRI
                     KGGATFELGTKTVTIVDAPGHTPDSIAVLVEGHLFTGDALFAGGAGRTDFMGGSSSDL
                     FDTLRGFESLPDATVVHPGHDYVGRAVTTIGEEKASNPLLREPDRAVFVARLSAKSAP
                     PANMAAILRHNLGEADAATITAEELQALRQQGSGPLLLDVRSPIEFESERIEGALNVP
                     LDALD"
     gene            929..3223
                     /locus_tag="VFX87_00150"
     CDS             929..3223
                     /locus_tag="VFX87_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX4993338.1"
                     /translation="MPVTDPVCGMDVTPGDAAGGSAEHAGTTYWFCNPSCRERFVADP
                     ARYLRPPEPAGKDTRVYTCPMHPEVRQVGPGSCPKCGLALEPLEVTADEGPNHELVDM
                     TRRFWVSLALTVPVLALAMGEMLAPGPVAALGPAAWLWSQLALSAPVVLWGGWPFFVR
                     GGQSLVTRNLNMFTLIALGTGAAFAYSVFAVLFPGALPHAMRHGGVPPVYFEASAVIT
                     TLVLLGQVLELRARSATSGAIRALLGLQPKAARRLRQDGREEDVPLEHVAVGDRLRVR
                     PGERVPVDGVVLDGRSAVDEALVTGEPIPVEKEAGSRVTGGTVNGTGGFVMRADRVGA
                     DTLLAQIVRMVGEAQRSRAPIQRLADQVSAWFVPAVVAVAVLTALAWGLLGPEPRLAY
                     ALVNLVAVLIIACPCALGLATPMSIMVATGRGALAGVLIKHAEALETLEKVDTLLVDK
                     TGTLTEGKPRLVSVVAADGATGAVTDAKILALAAGLERGSEHPLAAAILAGAAARGVA
                     PASVDAFRSLTGRGVLGEAAGRRAALGNARLLEELGIDAGALGKRAEALRDEGQTVMF
                     LVAADAVVGLLGVADPIKASARRAIRDLQGEGLRVVMVTGDSVATARAVARTLGLDDV
                     SAEILPERKAEIVRRFQAEGRVVAMAGDGVNDAPALAAADVGIAMGTGTDVAMESAGI
                     TLVQGDLGGIVRARRLSRATMRNIRQNLAWAFVYNVLGVPVAAGALYPLAGLLLSPMI
                     ASAAMSLSSVSVIANALRLRRAAL"
     gene            3352..4056
                     /locus_tag="VFX87_00155"
     CDS             3352..4056
                     /locus_tag="VFX87_00155"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4993339.1"
                     /translation="MNASRAVEPEQDARRCPRQQVSWLVTVAAGSRQFQGRTKDISAS
                     GAKILLKERPALGVEVGLRFRPPGRRPVETRALVWRLDSEGLACMFVGTQKPEFLEAV
                     TPRVVPGTVRVPPPTPAGAALLATVDTGFRALALDTLGRSGYTVLDAGSQPLLALRIA
                     EEHRDAITLILIASDLRLMNGEPLAKRLAPLLPSAKLVLMSSRSTPNPAGPGARWLPT
                     PCTDGELMAQIRQAQA"
     gene            complement(4075..4551)
                     /locus_tag="VFX87_00160"
     CDS             complement(4075..4551)
                     /locus_tag="VFX87_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4993340.1"
                     /translation="MTEAEALAALRGDDPGLAARAEATLWEIWCRSENPEVDRLFRQG
                     IEAMERRELEDAIAHFTRIIERAPDFAEGWNKRATARYVAEDYPGAVADCEETLARNP
                     HHFGALSGQGLCHMALGQFREAAALFRRALTVHPHLTSANHNLAAALSEAVKGNGH"
     gene            4611..4934
                     /locus_tag="VFX87_00165"
     CDS             4611..4934
                     /locus_tag="VFX87_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4993341.1"
                     /translation="MADQDRIEEFKEVVEIMPDDPVVRFGLAGAYLDAGQADAAVVEY
                     GLAIRLKPDYSAAHRGLGRALERAGRIAEAKAAYAKGLEVARATGDLQTGKEIEVFLR
                     RLEKA"
     gene            4935..5393
                     /locus_tag="VFX87_00170"
     CDS             4935..5393
                     /locus_tag="VFX87_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008986944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEX4993342.1"
                     /translation="MAIWVDADACPQVIKEILYRAAERAEMLTTLVANTSLRIPPSPF
                     IRTLRVEQGWDVADHRIVQQVEPGDLVVTADIPLAALVIARGAQALDPRGELYTEDNV
                     QERLAVRNLMQGLRAGGDLIAGPAPLSRSDRQRFANHLDHFLTRRRGPGD"
     gene            complement(5390..5944)
                     /locus_tag="VFX87_00175"
     CDS             complement(5390..5944)
                     /locus_tag="VFX87_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX4993343.1"
                     /translation="MAQNATIFKVTLEVADIDRAYYHDHALTVARHPSETDERMMVRL
                     LAFARHAHEALAFGRGLGTDDEPDLWRKDLTGAIDLWIDVGQPDEKRVRRACGRAETV
                     FVYSYGGRGADLWWEQAGAALDRAENLTVVNLPFAATRALAKLAQRNMQLTCTIQDGQ
                     IWMADSQQSVAIEPAVLQSPPAPG"
     gene            6068..7612
                     /locus_tag="VFX87_00180"
     CDS             6068..7612
                     /locus_tag="VFX87_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4993344.1"
                     /translation="MIVPLTLADFLERAELVYGHREAVVDEPTPPGGGLGRFTYARFA
                     SMARSLAASLDDLGVGENARVAIVSPNAARFLISLFGVSVFGRVLVPVNFRLNAEETR
                     YIIEHSGSSVLLVDPELDESLRDIKVKHRFVLGSGTDPQLFLRQGATPRLRVSDENAT
                     VSINYTSGTTARPKGVQLTHRNFWLNAVTFGWHLAMTDRDVYLHTLPMFHCNGWGMPY
                     ALTAMGVRHVIIRKIDGEEILRRVEGEGVTLFNCAPAVIAAVLDAAAVRRGKGVAVPG
                     KGRVRVVIAGAPPPTKTIERIETELGWEFIQIYGLTETSPLLTINRAPAEWDGLDPVE
                     RAQRLGRAGVPAVGVRMSVDAEGEVLARSNHVFEGYWNQPEETAKAIVDGWFHTGDGG
                     YADDAFIAISDRKKDVIITGGENVSSIEVEDCLFQHPAVAEVAVIGVPDEKWGETIKA
                     LVVLRPGAHCSERDLMEHCRSRMTHYKCPTSVEIRDALVRTATGKLQKFKLREPYWAG
                     RTRRVN"
     gene            7734..9077
                     /locus_tag="VFX87_00185"
     CDS             7734..9077
                     /locus_tag="VFX87_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX4993345.1"
                     /translation="MDDLRADLPVAVIGAGPVGLAAAAHLAVKRQKFIVLEAGTAVGA
                     FVRAWGHVQVFSPWRFNVDPAARSLLAASGWIEPDGDVLPTGAEIVAQYLEPLAKCPE
                     IAPHLRLGTRVVAVARSGHDKMKSAGREQAPFELHVRTAAGDDRLLARAVIDASGTYG
                     VPNPLGGGGLAAIGEDAARDRIFYGIPDVLGAARARYAGRSVAVVGSGHSAFNALLDL
                     ARLAASEPATTITWVVRRAEMGTIYGGGVADALPARGGLGERLRALVEAGRIRLVTGF
                     RTSRLLRRGAAVALLDMEGREIGPVDEIVAVTGFRPDLAMLGELRLDLDPAVESPRAL
                     APLIDPNLHSCGTVPPHGADELRHPEPGLFVVGMKSYGRAPTFLMLTGYEQVRSVVAE
                     LTGDLVGARRVELTLPATGVCSAAPGAAEDGAGCCASTSTPAPTLLGGLGRKPAR"
     gene            9074..9382
                     /locus_tag="VFX87_00190"
     CDS             9074..9382
                     /locus_tag="VFX87_00190"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4993346.1"
                     /translation="MKTIARLPLTQLQASDEHVEAFKALAHLTRLQVFFCLVHAGKEL
                     SVGEIQAAVEIPGPTLSHHLDLLRRAGLVESRKEERYIYYSVKREAVTTLVRLLTACC
                     "
     gene            9395..9835
                     /locus_tag="VFX87_00195"
     CDS             9395..9835
                     /locus_tag="VFX87_00195"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX4993347.1"
                     /translation="MSARVHLHLHVSDLAKSRTFYEKFLGAGPVKVKPGYVKFLPEWA
                     PVNLALSGGGAAGAGTIDHVGVQVDTVPSVMAQLARVKAAGLPVREEMGVNCCHANQD
                     KFWVQDPDGVEWEVYHLNYDLETEVTAPRGLGLPLAKGGSCCAS"
     gene            9847..10662
                     /locus_tag="VFX87_00200"
     CDS             9847..10662
                     /locus_tag="VFX87_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX4993348.1"
                     /translation="MSDDIKGIVKEKYARAALRVTAAGSTSCCGTASSRGECDPITSG
                     IYAESDTSALPAEARAASLGCGNPTALAELKPGEIVLDLGSGGGIDVLLSARRVGPSG
                     KAYGLDMTDEMLALARENQRKAGVGNVEFLKGEIEQIPLPSNTVDVIISNCVINLSAD
                     KDRVFAEAFRVLKPGGRLAVSDVVVRGEVPAEIRRSVELWIGCVAGALEEREYRDKLA
                     AAGFEGIDLEPTRVYRVEEAREFLTGAGLDVDAIAPQVADRFMSAFVRARKPA"
     gene            10771..>11011
                     /locus_tag="VFX87_00205"
     CDS             10771..>11011
                     /locus_tag="VFX87_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993349.1"
                     /translation="MSLFAPGGPAWLLAICVSRVGAYMVYIVYAATLPVLQHEWRLSG
                     TAAGSIASAFQVAYAVSLMGCSALADRVGARRVFLV"
BASE COUNT         1500 a         3778 c         4097 g         1636 t
ORIGIN      
        1 atcgagcgcg tccaggggca cgttgagcgc accctcgatg cgctcgctct cgaactcgat
       61 ggggcttcgc acgtcgagga gcagcggccc ggagccctgc tggcgaagcg cctggagctc
      121 ctcggccgtg atcgtcgccg cgtcggcctc gccgagattg tgccgcagga tggccgccat
      181 gttggcggga ggcgcggact tcgccgagag ccgggcgacg aagacggcac ggtccggctc
      241 gcggagcagg ggattgctcg ccttctcctc gccgatcgtg gtcacggccc gcccgacata
      301 gtcgtgaccg ggatgcacca ccgtggcgtc gggcaggctc tcgaagcccc ggagcgtgtc
      361 gaagaggtcg gaggagctgc cccccatgaa gtcggtccgg cccgcgccac cggcgaacag
      421 cgcgtccccc gtgaagagat ggccctccac gagcaccgcg atggagtccg gcgtgtggcc
      481 cggggcgtcg acgatcgtca ccgtcttcgt gccgagctcg aaggtcgcgc cgcccttgat
      541 ccgccgcacc ggacccagga gcttcgaggc cgcgtgcgcc agcacggtcg cgccggtccg
      601 tcgggcgagc cggcgcaccc cggagagatg gtcggcgtgc gtgtgggtgt cgaggacaaa
      661 ggtcagacgc gcatcgtgcg tcgcgagcgc ctcgacgaac cggtcgacct ggtccagccg
      721 gggatcgatc gccagggcgg tgcgacgggt ctcgtccacg accagataac ccaggcagcc
      781 gtcgcccggg gccgcaaagg tatcgatcgc gagaacgtcc gccgggctcg tgagcagcgt
      841 cattggctga tcctcctcgg gcgactatat aactacatac tcaaatagcc aagcgcgggc
      901 cggttcgcgg atgttgtagg atgacgccat gcccgtgacg gacccggtct gtgggatgga
      961 cgtgacgccg ggcgacgcgg ccggcggtag cgcggagcac gcgggtacca cctactggtt
     1021 ctgcaacccg agctgccggg agcggttcgt cgccgatccc gcccgctacc tccgcccgcc
     1081 cgagccggcg ggcaaggaca cgcgcgttta cacgtgtccc atgcacccgg aggtgcgcca
     1141 ggtcggaccc gggagctgcc ccaagtgcgg cctggcgctc gagccgctcg aggtcaccgc
     1201 cgacgagggt ccgaaccacg agctcgtgga catgacccgg cggttctggg tgagcctggc
     1261 cctgaccgtg cccgtcctcg cgctggcgat gggggagatg ctggcgccgg ggccggtggc
     1321 cgcgctcggc cccgccgcgt ggctctggag ccagctcgcc ctgtccgcgc cggtcgtgct
     1381 ctggggcggc tggccgttct tcgtgcgcgg cgggcagtcg ctggtcacgc gcaacctcaa
     1441 catgttcacg ctcatcgcgc tcggcaccgg cgccgcgttc gcgtacagcg tcttcgcggt
     1501 gctgttcccg ggcgcgctcc cgcacgcgat gcgccacggc ggcgtgccgc cagtgtactt
     1561 cgaggcgtcg gccgtgatca cgaccctggt gctactcggc caggtgctgg agctgcgggc
     1621 ccgcagcgcg acgtccggcg ccattcgcgc gctgctgggc cttcagccca aggcggcccg
     1681 gcggcttcgc caggacggcc gcgaggagga cgtgccgctc gagcacgtgg ccgtgggcga
     1741 ccggctgcgg gtgcggccgg gcgagcgcgt gccggtggac ggcgtcgtgc tggacggccg
     1801 gagcgcggtg gacgaggccc tggtcaccgg cgagccgatc ccggtggaga aggaggcggg
     1861 cagccgcgtg accggcggca ccgtcaacgg caccggcggc ttcgtgatgc gcgcggaccg
     1921 cgtcggcgcc gacacgctgc tggcccagat cgttcgcatg gtcggcgagg cgcagcgcag
     1981 ccgcgcgccc atccagcgcc tggccgacca ggtctccgcc tggttcgtcc cggcggtggt
     2041 ggcggtcgcg gtgctgaccg cgctcgcgtg gggactcctc gggccggagc cccgtctggc
     2101 ctacgcgctc gtcaacttgg tggcggtgct gatcatcgcg tgcccgtgcg ccctcggcct
     2161 ggcgacgccg atgtcgatca tggtggcgac cggccgcggc gctctggccg gcgtgctgat
     2221 caagcacgcg gaagcgctcg agacgctgga gaaggtggat acgctgctgg tggacaagac
     2281 gggcacgctg acggaaggca agccgcgcct cgtctcggtg gtcgcggccg acggcgcgac
     2341 tggcgccgtg accgacgcga agatcctggc gctggccgcc gggctcgagc gcggtagcga
     2401 gcatccgctg gccgccgcca tcctggccgg cgccgccgcg cgtggcgtgg cgccggcgtc
     2461 cgttgacgcg tttcgctcgc tcaccgggcg cggggtgctc ggcgaggccg cgggccgtcg
     2521 cgcggccctc ggcaacgcgc gcctgctcga ggagctgggc atcgacgcgg gcgcgctcgg
     2581 caagcgggcg gaggccctgc gcgacgaggg ccagaccgtg atgttcctgg tcgcggccga
     2641 cgcggtcgtg ggccttctcg gggtggccga tcccatcaag gcgagcgcgc gccgggcgat
     2701 tcgcgatctc cagggcgagg gcctgcgggt ggtcatggtc accggcgaca gcgtggccac
     2761 cgcgcgggcg gtggcccgca cgctcggcct cgacgacgtg agcgccgaga tcctgcccga
     2821 gcggaaggcc gagatcgtcc ggcgcttcca ggccgagggc cgggtggtcg ccatggccgg
     2881 tgacggcgtc aacgacgccc cggccctcgc ggcggccgac gtgggcatcg ccatgggcac
     2941 gggcacggac gtggccatgg agagcgccgg catcacgctg gtccagggcg acctgggcgg
     3001 catcgtgcgg gcgcgccggc tctcgcgcgc gaccatgcgg aacatccgcc agaatctcgc
     3061 ctgggccttc gtgtacaacg tcctcggcgt gccggtcgcc gcgggcgcgc tctacccgct
     3121 cgccggcctt ctgctcagcc cgatgatcgc gagcgcggcg atgagcctca gctcggtgtc
     3181 ggtcatcgcc aacgccctgc ggctccgccg ggcggcgctc tgagtgtcaa cggcgccgtc
     3241 gcgacgctga cagcagcctg acattgcctt cgtgcgtcac gtccgaaggc gttgtatcgc
     3301 aagcagttat cgtctggcgc gcttcttgcg attccccccg gacacgcagc catgaacgcg
     3361 agccgagcgg tcgaacccga gcaggacgcg cggcgatgcc cgcgccagca ggtctcctgg
     3421 ctcgtcacag tggccgcggg ctcgcggcag ttccagggac ggacgaagga catcagcgcg
     3481 tccggcgcca agatcctgct caaggagcgg ccggcgctcg gcgtcgaggt tggcctgcgc
     3541 ttccgtcctc ccggccgccg gcccgtagag acgcgggcgc tggtgtggcg gctcgattcc
     3601 gagggactgg cgtgcatgtt cgtgggcacg cagaagccgg aattcctgga agccgtgacc
     3661 ccgcgcgtgg tgccggggac ggtccgcgtg cccccgccga caccggccgg cgccgcgctc
     3721 ctggcgaccg tcgatacagg ctttcgcgcg ctggcgctcg acacgctcgg gcgcagcggc
     3781 tacacggtgc tggacgcggg atcgcagccg ctgctggccc tgcgcatcgc ggaggagcac
     3841 cgggacgcga tcacgctcat cctgatcgcg tcggacctgc ggctcatgaa cggtgagccg
     3901 ctggccaagc gcctcgcccc gctcctgccg tcggccaagc tcgtgctgat gtcgagccgc
     3961 tcgacgccga atccggccgg ccccggggcg cggtggctcc cgacgccgtg caccgacggc
     4021 gagctgatgg cgcagatccg ccaggcccaa gcgtaggacc gccgcgccgc ccggctagtg
     4081 cccgttgccc ttgaccgcct cggacagggc tgcggcgagg ttgtggttgg ccgaggtcag
     4141 gtgcgggtga acggtcaggg ctcgccggaa cagcgccgcg gcctcccgga actggcccag
     4201 cgccatgtga cagaggccct ggccggagag cgcgccgaag tgatggggat tccgcgctag
     4261 cgtctcctcg cagtcggcca ccgctcccgg gtagtcctcc gccacgtagc gcgcggtcgc
     4321 ccgcttgttc catccttcgg cgaagtccgg cgcgcgctcg atgatgcggg tgaagtgggc
     4381 gatcgcgtcc tccagctcgc gacgctccat cgcctcgatg ccctgacgga agagccgatc
     4441 cacctcgggg ttttccgaac ggcaccagat ctcccagagc gtggcctcgg cccgggcggc
     4501 caggcccggg tcatcgccac ggagcgctgc gagggcttca gcctcggtca tgcgcccgag
     4561 gttacttgac ggggagcccg gttggccaga tgctgctgcc gggaggaccg atggcggatc
     4621 aggatcggat cgaggagttc aaggaagtgg tcgagatcat gccggacgat ccggtcgtcc
     4681 gcttcggcct cgcgggtgcc tatctcgacg cgggccaggc cgacgcggcg gtcgtggagt
     4741 acggcctggc cattcgcctc aagccggact actcggcggc tcaccgcggc ctcggccggg
     4801 cgctcgagcg cgcgggccgg atcgcggaag ccaaggccgc gtacgcgaag ggtctcgagg
     4861 tggcgcgcgc gaccggggat ctccagaccg ggaaggagat cgaggtcttc ctgcgccggc
     4921 tcgagaaggc ataagtggcg atctgggtgg atgccgacgc ctgcccccag gtcatcaagg
     4981 agatcctgta ccgggccgcg gagcgcgcgg agatgctgac gacgctggtc gccaacacgt
     5041 ccctccgcat cccgccgtcg cccttcatcc ggaccctccg cgtggagcag ggctgggacg
     5101 tggcggatca ccggatcgtg cagcaggtgg agcccgggga tctcgtggtc accgcggaca
     5161 tccccctcgc cgcgctggtg atcgcgcgcg gcgcccaggc gctggacccg cgcggggagc
     5221 tctacaccga ggacaatgtc caggagcgcc tggccgtgcg gaacctgatg cagggcctgc
     5281 gcgcgggcgg cgacctcatc gccggcccgg cgcccttgtc ccggagcgac cggcagcgct
     5341 tcgcgaatca cctcgaccac ttcctgacgc gacgccgcgg ccccggcgac tagccgggcg
     5401 ccggcgggct ctgcagcacc gccggctcga tcgcgacgct ctgctgcgag tccgccatcc
     5461 agatctgacc gtcctggatc gtgcacgtga gctgcatgtt ccgctgcgcc agcttcgcaa
     5521 gcgcccgcgt ggcggcgaag ggcaggttga cgacggtcag gttctcggcc cggtcgaggg
     5581 cggcgccggc ctgctcccac cagagatcgg cgccccgccc gccgtagctg tagacaaaca
     5641 ccgtctccgc gcggccgcag gcccgccgga cgcgtttctc gtcgggctgg ccgacgtcga
     5701 tccagagatc gatggcgccg gtgaggtcct tccgccagag atcgggctcg tcgtcggtgc
     5761 cgagcccgcg gccgaaggcc agcgcctcgt gcgcgtggcg ggcgaaggcc agcagccgca
     5821 ccatcatgcg ctcgtccgtc tccgacgggt ggcgggcgac cgtgagggcg tggtcgtggt
     5881 agtaggcgcg gtcgatgtcc gcgacctcca gggtgacctt gaagatggtg gcgttctggg
     5941 ccatggcgcc gcgcgcagag ccggcgtggc gatcgtctcc cggcgggcgc gcgcggtcaa
     6001 ggaaggcgtg ggccggattg cgcggcgggg gcgccttgat ctatcctccg tcggggggta
     6061 cggaaccatg atcgttccgc tcacgctggc cgattttctc gagcgcgccg agctcgtcta
     6121 cggacaccgc gaggcggtgg tggacgagcc cactccgccc ggcggcggcc tcggccgctt
     6181 cacctacgcg cggttcgcct cgatggcgcg aagcctggcc gcctcgctgg acgacctcgg
     6241 cgtcggggag aatgcccgcg tcgcgatcgt gtcgccgaac gcggcgcgct tcctcatcag
     6301 cctcttcggg gtcagcgtgt tcggccgcgt gctggtgccg gtgaacttcc ggctgaatgc
     6361 cgaggagacc cgctacatca tcgagcactc gggctcctcg gtgctcctgg tggatcccga
     6421 gctggacgag tccctgcgcg acatcaaggt gaagcaccgg ttcgtgctgg gcagcggcac
     6481 cgacccgcag ctcttcctgc gccagggcgc aactccgcgc ctgcgcgtca gcgacgagaa
     6541 cgccaccgtg tccatcaact acacctccgg caccaccgcg cggcccaagg gcgtgcagct
     6601 cacccatcgc aacttctggc tcaacgcggt cacgttcggc tggcacctgg ccatgacgga
     6661 ccgggacgtg tatctccaca cgctgcccat gttccactgc aatggctggg gcatgcccta
     6721 cgcgctcacc gccatggggg ttcgccacgt catcatccgg aagatcgacg gcgaggagat
     6781 cctgcgccgc gtggagggcg aaggggtcac gctcttcaac tgcgcgccgg cggtcatcgc
     6841 ggcggtgctc gacgcggccg cggtccgtcg cggcaagggc gtggcggtgc ccgggaaggg
     6901 gcgcgtgcgg gtggtcatcg cgggagcgcc gccgccgacc aagaccatcg agcggatcga
     6961 gaccgagctg ggctgggaat tcatccagat ctacggcctc accgagacct cgccgctctt
     7021 gacgatcaac cgggcgcccg cggagtggga cgggctcgac ccggtcgagc gcgcgcagcg
     7081 gctcggccgg gcgggcgtgc ccgcggtggg tgtgcgcatg agcgtggacg ccgagggcga
     7141 ggtgctcgcg cgctccaacc acgtcttcga ggggtactgg aaccagccgg aggagacggc
     7201 caaggccatc gtggacggat ggttccacac cggcgacggc ggctacgccg acgacgcctt
     7261 catcgcgatc agcgaccgga agaaggacgt gatcatcacc ggcggcgaga acgtctcgtc
     7321 catcgaggtg gaggactgcc tcttccagca cccggcggtg gccgaggtgg cggtgatcgg
     7381 cgtgcccgac gagaagtggg gcgagacgat caaggccctc gtggtcctgc gcccgggcgc
     7441 ccactgcagc gagcgggacc tgatggagca ttgccggagc cgcatgaccc actacaagtg
     7501 cccgacctcg gtcgagatcc gggacgcgct cgtccgcacc gccaccggca agctccagaa
     7561 gttcaagctc cgcgagccct actgggccgg tcgcacccgc cgcgtgaact gacgcccggg
     7621 gccggggcgt caacctgcgc cgcaagaaga ggcttgactc cggttttata tttcgatata
     7681 tctcgaaata cagaataaga cctcgcgggg gagggcaagg ggagatgtcg cccatggatg
     7741 acctgagggc agacctgccg gtggcggtga tcggggcggg cccggtgggc ctggccgcgg
     7801 cggctcatct ggccgtgaag cggcagaagt tcatcgtcct ggaagcgggc accgcggtgg
     7861 gcgccttcgt ccgggcctgg ggtcatgtcc aggtgttctc gccgtggcgg ttcaacgtgg
     7921 acccggcggc ccggagcctg ctggcggcct cggggtggat cgagcccgac ggcgacgtgc
     7981 tgccgaccgg cgccgagatc gtcgcccagt acctcgagcc cctggcgaag tgccccgaga
     8041 tcgcgccaca cctgcgcctc gggacgcgcg tggtggcggt ggcccgctcc ggccacgaca
     8101 agatgaagag cgcgggacgg gagcaggcgc cgttcgagct gcacgtccgg actgcggccg
     8161 gtgacgacag gcttctggcc cgcgccgtga tcgatgcctc ggggacctac ggtgttccca
     8221 atccgctggg cggaggcggc ctcgctgcga tcggggagga cgccgctcgc gaccggatct
     8281 tctacgggat cccggacgtg ctgggcgccg cccgtgcgcg ttacgcgggc cggtccgtgg
     8341 cggtggtcgg cagcgggcat tcggcgttca acgcgctgct cgatctggcc cggctcgctg
     8401 cgagcgagcc cgccaccacg atcacgtggg tggtgaggcg cgcggagatg ggcacgatct
     8461 atggcggtgg ggtcgccgat gcgctgcccg cgcggggggg cctgggcgag cgcctgcgcg
     8521 cgctggtcga ggccgggcgg atccgcctcg tgaccgggtt ccgcacgagc cggctgctgc
     8581 gccgcggggc cgcggtggcg ctgctcgaca tggaggggcg ggagatcggt ccggtggatg
     8641 agatcgtcgc ggtcaccggc ttccgcccgg acctggcgat gctcggcgag ctgcgcctcg
     8701 atctcgatcc ggcggtggag agcccgcgcg cgctggcccc gctgatcgac ccgaacctgc
     8761 atagctgtgg cacggtgccg ccccacggcg ccgatgagct ccgtcacccg gagccgggcc
     8821 tcttcgtcgt cggcatgaag agctacggcc gggcgccgac cttcctgatg ctcaccggct
     8881 acgagcaggt gcggtcggtg gtcgcggagc tgaccggtga cctcgtcggg gcgcgacggg
     8941 tggagctgac gctgccggcc accggggtct gctccgccgc gccgggtgcg gcggaggacg
     9001 gcgcggggtg ttgcgcctcg acctcgaccc cggcgcccac gctgctcggt ggattgggcc
     9061 ggaagccggc tcgatgaaga ccatcgcgcg ccttccgctg acccagctcc aggcgagcga
     9121 cgaacacgtc gaggcgttca aggccctggc gcacctcacc cggcttcagg tcttcttctg
     9181 cctggtccac gcgggcaagg agctgtcggt gggcgagatc caggccgcgg tcgagattcc
     9241 gggcccgacg ctgtcgcacc atctcgacct gctccgccgg gccgggctgg tcgagagccg
     9301 caaggaagag cggtacatct actattccgt gaagcgggaa gcggtgacga cgctcgtgcg
     9361 tctgctcacc gcctgctgct gagaggagga tcctatgagc gcgcgcgtcc atctccatct
     9421 ccacgtgtcg gatctggcca agagccggac gttctacgag aagtttctcg gggcggggcc
     9481 ggtcaaggtg aagcccgggt acgtgaagtt ccttcccgaa tgggcccccg tcaatctggc
     9541 cctctccggt ggcggggcgg ccggcgcggg cacgatcgat cacgtgggcg tgcaggtgga
     9601 cacggtgcct tcggtgatgg cccagctcgc ccgcgtcaag gcggccgggc tgccggtgcg
     9661 ggaggagatg ggcgtgaact gctgccacgc gaaccaggac aaattctggg tgcaggatcc
     9721 ggacggcgtc gagtgggaag tctatcacct gaactacgac ctcgagaccg aggtgacggc
     9781 gcctcggggc ctggggctgc cgctggccaa gggcggctcc tgctgcgcct cgtgaggaga
     9841 tgagagatga gcgacgacat caagggaatc gtgaaggaaa agtacgcgcg ggccgcgctc
     9901 cgagtgaccg cggccggtag cacctcctgc tgcggcaccg cctcttctcg cggcgaatgc
     9961 gatccgatca cctccggtat ctacgcggag agcgacacct ccgcgctgcc ggccgaggcc
    10021 cgggccgcct ctctcggctg cgggaacccg acggcgctgg cggagctcaa gccgggcgag
    10081 atcgttctgg acctcggatc ggggggcggc atcgacgtgc tcctctccgc ccgccgcgtg
    10141 ggcccgtcgg gcaaggcgta cggcctcgac atgaccgacg agatgctcgc ccttgcccgc
    10201 gagaatcagc ggaaggccgg cgtgggcaac gtggagttcc tcaagggcga gatcgagcag
    10261 atccctctcc cgtcgaacac ggtggacgtc atcatctcca actgtgtgat caatctctcg
    10321 gccgacaagg atcgcgtctt tgccgaggca ttccgggtcc tgaagccggg cgggcgcctg
    10381 gccgtgtcgg acgtggtcgt gcgcggggag gttcccgcgg agatccggcg gagcgtcgag
    10441 ctctggatcg gctgcgtggc cggcgcgctg gaagagcgag agtatcgaga caagctcgcg
    10501 gcggcgggtt tcgaggggat cgacctggag cccactcgcg tgtaccgtgt cgaggaggcc
    10561 cgggaattcc tgacgggcgc cgggctcgac gtggacgcca tcgcgcccca ggtggccgac
    10621 cggttcatga gcgcgttcgt ccgcgcccgc aagcccgcgt aacgtgtcgc cggttgtcga
    10681 tcgggcacgc ctcggtcggc gggtggcggc ggaggcggga gggaccgcgt ccgcgtccgg
    10741 gctgtttcac cgcctcccac ccagccgcag atgagcctgt tcgctccggg cggccccgcg
    10801 tggctgctcg cgatctgcgt gtcgcgagtc ggagcctaca tggtgtacat cgtctacgcg
    10861 gcgacgctgc ccgtcctgca gcacgagtgg cggctctccg gcaccgcggc gggcagcatc
    10921 gcgtccgcgt tccaggtcgc gtacgcggtg tcgctgatgg ggtgctccgc gctggcggat
    10981 cgagtaggcg cgcggcgcgt cttcctggtc g
//
LOCUS       DASUFA010000007         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1033442, whole genome shotgun sequence.
ACCESSION   DASUFA010000007 DASUFA010000000
VERSION     DASUFA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033442"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1526)
                     /locus_tag="VFX87_00210"
     CDS             complement(90..1526)
                     /locus_tag="VFX87_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX4993350.1"
                     /translation="MKWVAPRSGFDEVFDHRGHPRAHYQALISILESFTKEDVERRER
                     LQQLTLRSQGITFTVYGEKDGLERIFPFDFVPRIIPAAEWKGLQDGLVQRITTLNLFL
                     ADIYQDQKCIKDGIVPAELILSRKEYKRQLLGVIPARKIFTHVVGTDIIRDDKGEYLV
                     LEDNCRVPSGVSYVLENRNLLSRVFPEFLGSYPVRPIKDYPTLLLETLRFVAPRRGQN
                     PMVVVLSPGIHNSAYFEHSFLAREMGVPLVEGRDLLVEGNHVFMRRTHGKRQVDVIYR
                     RIDDDFLDPLAFRRDSMLGVPGLLNVYRESNVALANAPGAGVADDKSVYAFMPHLIKY
                     YLGEDALLPQVPTYLGFRKDDFKYISEHLDRLVVKTTGDAGGYGMLMGPFAAAKEREG
                     YLAAMKKNPGNYIAQPLVQLSTHPTHIEGRFEPRRVDLRPFILYGDRIRVLPGGLTRV
                     ALRAGSYVVNSSQGGGSKDTWVLAPRDA"
     gene            1619..2170
                     /locus_tag="VFX87_00215"
     CDS             1619..2170
                     /locus_tag="VFX87_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4993351.1"
                     /translation="MSADAAQPPHTHLTPDTIRFCPLCGASLVRQPVGPERRPEMVCP
                     GCGFIYYVNQKVVAGTIPWQDGRLLLARRAIHPAHGKWTFPGGYVDWGETVDAGALRE
                     TWEETGLTVELGELVGVYSYAGAPVVIVVYEAVVTGGSLTVCHENDRLEWVTPAEVPW
                     DELAFPSTVAAVRDFLARRTARD"
     gene            complement(2335..2754)
                     /locus_tag="VFX87_00220"
     CDS             complement(2335..2754)
                     /locus_tag="VFX87_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX4993352.1"
                     /translation="MPLEPGQAATVERVVTRELTADALGNPGVTVFATPFVINLLEHA
                     AHAVMVPYLTPGAATVGTGVEVRHLAATPPGMRVRATAVLRETDGRRCLFEVEAWDEV
                     EKIAEGRHERFMVADLARFLARAAAKADRAKGSSRAS"
     gene            2820..4001
                     /locus_tag="VFX87_00225"
     CDS             2820..4001
                     /locus_tag="VFX87_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993353.1"
                     /translation="MPVLLAALGALLVSLDSAINIALPAMASAFGVGPAAIRWVIICY
                     VLTYAFTAFAAGLLADRLGPGPVFTAGLWLCAASFVGYALASSYGTVLLLRVAQGVGS
                     GMVYGTAPALVTLSLPPERHGRGLGTMSLGIGVGLGVGPVLGGLLVDGFGWPAAFVFR
                     APLFLVVALLALTRFGRVAGAAPTPRRLAVADLLRLPVLRAAALAFLSQHAQFAVWLL
                     MPFFLVTARGLSPSVGGTVFVLTPLATALAAPLGGALTDRIGPRWPLRIGLALEAAGL
                     LAIGGVTEATPIALLGAAMALVGFGAGLFQVPNLALMMAAFPPAQQGAAGGLAFLGRT
                     LGSAVGVQVTALLFDARLPRDGFLAAFHAAFLAAGLVCVLAAGLALLPARSSAPRGVA
                     R"
     gene            4015..>4284
                     /locus_tag="VFX87_00230"
     CDS             4015..>4284
                     /locus_tag="VFX87_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993354.1"
                     /translation="MSVPGSPYRWAILAVCVAGFMQTHVHRVGFAPLIPTFIADMGLT
                     YAAAGTIMAAYFWSYAVMQVPVGVLTDRLGARRVMLGCMGVMALGA"
BASE COUNT          503 a         1534 c         1540 g          707 t
ORIGIN      
        1 ctcggtcacc agcaggagcc gggtggcgtt ctcggcccgc tccaggtagc gccccatcca
       61 gtagagcccg tccgcgacgc gggaaaggat catgcgtcgc gcggggccag cacccaggtg
      121 tccttgctgc ctccgccctg ggaggagttg accacgtagg agcccgcgcg cagggccacc
      181 cgggtgagcc cgcccggcag cacccggatc cggtcgccgt agaggatgaa cggccgcagg
      241 tccacccggc gcggctcgaa cctgccctcg atgtgggtgg gatgcgtgga gagctggacg
      301 agcggctggg cgatgtagtt gcccgggttc ttcttcatcg cggccaggta gccctcgcgc
      361 tccttggcgg ccgcgaacgg ccccatgagc atcccgtagc cgcccgcgtc tccggtcgtc
      421 ttcaccacga gccgatcgag gtgctcgctg atgtacttga agtcgtcctt ccggaacccg
      481 aggtaggtcg gcacctgggg caggagcgcg tcctcgccca ggtagtactt gatgaggtgc
      541 ggcatgaacg cgtagaccga cttgtcgtcg gccacccccg cgcccggcgc gttggcgagc
      601 gccacgttgc tctcgcggta cacgttgagc aggccgggca cacccagcat gctgtcgcgc
      661 cggaaggcga ggggatcgag gaagtcgtcg tcgatcctcc ggtagatcac gtccacctgc
      721 cgcttgccgt gggtgcgccg catgaagacg tggttgccct ccaccagcag gtcgcgcccc
      781 tccaccagcg gcacgcccat ctcgcgcgcg aggaacgagt gctcgaagta ggcggagttg
      841 tggatgcccg ggctcaggac gaccaccatc gggttctggc cacgccgcgg cgccacgaag
      901 cggagggtct cgagcagcag ggtcgggtag tccttgatcg ggcgcaccgg gtaggagccg
      961 aggaactcgg gaaacacgcg gctcaggagg ttgcggttct ccagcacgta ggagaccccg
     1021 ctcggcaccc ggcagttgtc ctcgaggacg aggtactcgc ccttgtcgtc gcggatgatg
     1081 tcggtgccga ccacgtgggt gaagatcttc cgggccggga tcacgcccag gagctgccgc
     1141 ttgtactcct tgcgggagag gatcagctcc gcgggcacga tcccgtcctt gatgcacttc
     1201 tggtcctggt agatgtcggc gaggaagagg ttcagcgtgg tgatgcgctg gacgaggccg
     1261 tcctggaggc ccttccactc cgcggcgggg atgatgcggg ggacgaagtc gaacgggaag
     1321 atccgctcga gcccgtcctt ctccccgtag accgtgaagg tgatgccctg gctgcgcagg
     1381 gtgagctgtt gcagccgctc gcggcgctcc acgtcctcct tggtgaagga ctcgaggatg
     1441 gagatgaggg cctggtagtg ggcacgggga tggccgcggt ggtcgaagac ctcgtcgaag
     1501 ccgctccggg gcgcgaccca cttcatggac cggtcgcctc ctttgaggct agagcctctc
     1561 ccgcggggcc gccgcgtgtc aaggcgacgc gggcccgcgt ggtagactga ccgtcgtcat
     1621 gtcggccgac gcggcccagc cgccccacac ccacctcacg ccggacacga tccgcttctg
     1681 ccccctctgc ggcgcgagcc tcgtccggca gccggtcggg cccgagaggc gcccggagat
     1741 ggtgtgcccc ggctgcgggt tcatctacta cgtgaaccag aaggtggtcg cgggcaccat
     1801 cccgtggcag gacggccgcc tcctgctcgc ccgccgggcg atccatcccg cccacggcaa
     1861 gtggacgttt cccggcggct acgtggactg gggcgagacg gtggacgcgg gcgcgctccg
     1921 cgagacgtgg gaggagaccg ggctcaccgt cgagctgggc gagctggtcg gcgtgtactc
     1981 ctacgcgggg gcgccggtgg tcatcgtggt gtacgaggcg gtcgtgacgg gtgggtccct
     2041 gaccgtctgc cacgagaacg accgcctcga gtgggtgacg cccgcggagg tcccgtggga
     2101 cgagctggcc ttcccgtcca cggtggcggc ggtgcgcgat ttcctggcgc gccggaccgc
     2161 ccgcgactga ccggacccgg ctcaaagcaa cgcgctaccc acgtgacctc agtgtcacgg
     2221 tcgctcgtcc ggtgaccatc ttctcgccct tctgattctc ggcccccacg cccgggtcct
     2281 tgatgttcac gcgtatcgcc atgggattgc gctcgcttcc ccgtcagacg cggctcagct
     2341 cgccctgctc gatcccttcg cccggtcggc cttggcggcg gcgcgcgcga ggaagcgggc
     2401 gaggtcggcc accatgaagc gctcgtgccg cccctccgcg atcttctcca cctcgtccca
     2461 cgcctcgacc tcgaagaggc agcggcggcc gtcggtctcc cgcaggaccg ccgtggcccg
     2521 cacgcgcatg ccggggggcg tggcggccag gtgccgcacc tcgaccccgg tacccacggt
     2581 ggccgccccc ggggtcaggt acgggaccat caccgcgtgg gccgcgtgct cgagcaggtt
     2641 gatgacgaac ggggtcgcga ataccgtgac gcccggattg ccgagggcgt cggcggtgag
     2701 ctcgcgcgtg acgacgcgct ccacggtggc ggcctgtccg ggctcgagcg gcatggggtg
     2761 tcttcttcta ccccgattgc cgcgccaggt caacggcccg ggccacgtat acttgcccgg
     2821 tgcccgtcct cctggccgcc ctcggcgcgc tgctcgtctc cctcgactcg gcgatcaaca
     2881 tcgcgctccc cgcgatggcc tccgccttcg gggtcgggcc ggcggcgatc cgctgggtca
     2941 tcatctgcta cgtcctcacc tacgcgttca ccgcgttcgc ggcgggcctg ctcgcagatc
     3001 ggctcggccc cgggccggtc ttcacggccg ggctctggct ctgcgcggcc agcttcgtgg
     3061 gctacgcgct cgcctcctcc tacggcacgg tgctgctgtt gcgcgtggcg cagggcgtgg
     3121 gcagcggtat ggtttacggc accgcgcccg cgctggtgac gctgtcgctc ccgcccgagc
     3181 gccacggccg cgggctcggc acgatgagcc tgggcatcgg cgtcgggctc ggggtgggcc
     3241 cggtgctggg cggcctgctc gtggacggct tcggctggcc cgccgccttc gtgttccgtg
     3301 cgccgctgtt cctagtcgtg gcgctcctcg ctctcacgcg cttcggccgg gtcgccggcg
     3361 ccgcccccac gccgcgccgg ctcgcggtgg ccgacctgct gcgcctgccc gtgctccgcg
     3421 ccgcggccct cgcgttcctc tcgcagcacg cgcaattcgc ggtctggctg ctgatgccgt
     3481 tcttcctggt caccgcgcgc ggcctctcgc ccagcgtggg cggcacggtc ttcgtgctca
     3541 ccccgctcgc caccgcgctg gcggcgccgc tgggcggggc gctgaccgat cgcatcggcc
     3601 cgcggtggcc gctgcggatc ggactcgccc tcgaggcggc cgggctgctc gcgatcggcg
     3661 gcgtcaccga ggcgacgccg atcgcgctcc tcggcgccgc catggccctg gtgggcttcg
     3721 gggccggcct cttccaggtg ccgaacctgg ccctgatgat ggccgcgttc ccgcccgcgc
     3781 agcagggcgc ggcgggcggg ctcgcgttcc tcgggcgaac gctggggagc gcggtgggcg
     3841 tgcaggtgac cgcgctgctc ttcgacgcgc ggctgccgcg cgacggcttc ctcgccgcct
     3901 tccacgcggc cttcctggcc gccggcctcg tgtgcgtcct cgcggcgggt ctggccctgc
     3961 tcccggcccg gtcgtcggcc ccgcgcgggg tcgcccgatg agcaaatctg gccggtgagc
     4021 gtccccggct cgccgtaccg gtgggcgatc ctcgcggtct gcgtggcggg cttcatgcag
     4081 acccacgtcc accgtgtcgg cttcgcgccg ctgatcccga ccttcatcgc ggacatgggg
     4141 ctcacctacg cggcggcggg aaccatcatg gccgcgtact tctggagcta cgcggtgatg
     4201 caggtcccgg tcggcgtgct caccgatcgg ctcggcgcgc ggcgcgtgat gctcgggtgc
     4261 atgggcgtga tggcgctggg cgcg
//
LOCUS       DASUFA010000008         9772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1146714, whole genome shotgun sequence.
ACCESSION   DASUFA010000008 DASUFA010000000
VERSION     DASUFA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146714"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..905)
                     /locus_tag="VFX87_00235"
     CDS             complement(42..905)
                     /locus_tag="VFX87_00235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4993355.1"
                     /translation="MPLKLGLFSMTTGPCTYPDGAARVARAAEAAGFDSLWGGEHVVL
                     PDPRVPPSPMEPEDRIMDPVVTLAFVAAHTTRIRLGTGIIILPQRNPLVLAKQLASLD
                     VLSGGRLIFGVGVGYLEPEFRALGIPFEHRGPVTDDYLAALRVIWSEARPAYRGRFVS
                     FDGVQAHPRPTQQPGPPLVVGGHTEAAFRRAVEQGHGWYGFALDEAGVQRCLEGLRQA
                     AARHERPAALGALEISVTPRPTRGGEAMDRASADRFAALGVHRLILMPPRSLDAGGLE
                     RFVEKAARELR"
     gene            complement(905..1747)
                     /locus_tag="VFX87_00240"
     CDS             complement(905..1747)
                     /locus_tag="VFX87_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4993356.1"
                     /translation="MGDWLETYRGTVFRWEVDPNDHLTVAYYFARIADAGAGLLAAVG
                     LTAAATPTADSFVRYQRELRVGDIMHMESGVISVELDALVLGHKLFDSGAGVLCTTVE
                     QRVRLAAPLTPETRRAVEARRVEWDGPARERRPRPPGLAGFRDTARDIVGPVEVDISG
                     GAALSHYIHRFSAANGHAIAAFGMTPAYMRAEHRGFSTFEFQLELGSPLHPGDGVRVR
                     SGILHVGNSSLRLLHVMTREPAGEVVATLEQSGVHFDQDARRPAPLPDVLRERARALL
                     VKDA"
     gene            complement(1761..2744)
                     /locus_tag="VFX87_00245"
     CDS             complement(1761..2744)
                     /locus_tag="VFX87_00245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4993357.1"
                     /translation="MERRILGKSSLAVSAIGLGCMSMSNVYGRGDEAESIAVVHRALD
                     LGVNFLDSSDMYGWGQNEELLGRALRGRRDQVVLATKFGNLRKPDGTAGVNGRPEYVP
                     QACDASLKRLGVSTIDLYLQHRVDPSVPIEETVGAMSRLVEAGKVRFLGLSEASPATV
                     RRAHAVYPITALQSEFSLLYRVEAEETLPTLRELGISFMAYSPLGRSLLTGRTRSAAE
                     IPSDDRRRDHPRFQEENLKKNLELLKPLDDMARKKGCTPGQLALAWLLARGPDIVPIP
                     GTKRKDRLEENVAAADVRLTPEEVAALQEAIPAGAAAGERYPVPAMKSVYI"
     gene            2824..4482
                     /locus_tag="VFX87_00250"
     CDS             2824..4482
                     /locus_tag="VFX87_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4993358.1"
                     /translation="MPHDVTLLRDIALGIVFAATLGHVARLLRQPLLLGYIAGGVLLS
                     SQMGFGLITNAESIELISEIGLILLLFIIGLEIDLRELQRLGRSMLVLGVGQFVINAL
                     LGLAFFAWLGYRVAEGRFDLIYLAIVMSLSSTLIVVKLLREKFELKILSGRLTLGVLV
                     VQDIWAILFMAVQPSLAEPGALQIAQSVAGGAVLVGVAFLASRYILAPLLEASARRPE
                     LVLISSVAWCFGIAGVAHQLGLSREMGALIAGVSISAFPYGADVISKVAGVRDFFVTL
                     FFVSLGLKVPVPSGDVLGHALLIVVFVLVSRVLSVVPTSYVLGDGLYAGMVTALNLAQ
                     ISEFSLVILTLGTGYGHVSERVSAVVLTAMILTSLLSPYLITGNDRIARWLLRPFVRG
                     PAAGRAAAPTAEARAPRDIVLLGHFRIAQAVLDLVEAQAPQLKERITLVDYDANRAHG
                     VMARGFRWEYGDLANPDALQHLGIEEARIVVTTISDTFLKGISTRRLVGNLRRLAPRA
                     VIVMTGEERTDMEDLLRAGADHVLIPGEITGERILELLRKGDAA"
     gene            complement(4493..5470)
                     /locus_tag="VFX87_00255"
     CDS             complement(4493..5470)
                     /locus_tag="VFX87_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993359.1"
                     /translation="MDISCAFPPVPGTPQHIALAERLGYRRAWVYDTPALQLDVWMTL
                     GRAAERTRRIELGPGVLIPSLRHVVVTAAAVATLVDIAPGRVNVGIGSGFTGRMAMGQ
                     RPNSWTSVALYARQLRGLLAGEMVEVDGALTQLLHGAGQAPRRPIRVPFVFGTRGPRG
                     EAVAHAEGDGVFSVVPTPGFAWSILLTLGTVLEPGERPDAPRVLDAAGAGAAVLLHRA
                     YEYRNVPGLDLATLPGGPAWQAAIEAVPARERHLRTHTGHLTELNPVDRAVVSGDLIR
                     RVTFTGEATALRARVAELAARGVTEIAYQPSGGDIPRELTAFARMTGIA"
     gene            complement(5485..6162)
                     /locus_tag="VFX87_00260"
     CDS             complement(5485..6162)
                     /locus_tag="VFX87_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4993360.1"
                     /translation="MELYDVMRTTFSAREFTADPLPDEILFKILDRARFAPSGGNRQG
                     WRVIVVREPKTREGLAVATVPAAKRYAAQVQVGESPWNTIDPTKVDAATIERTPAPPR
                     LIEPVLKAPVVLVVCVDLKVVASMDSELKRVGVISGASIYPFAWNILLAARHEGFAGT
                     ITTLATAQEPALQKLLGLPSHVAVAAVMPLGRPVKPITKLTRKPVGEFAMLERWGGAP
                     LGPGRPA"
     gene            6233..7432
                     /locus_tag="VFX87_00265"
     CDS             6233..7432
                     /locus_tag="VFX87_00265"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4993361.1"
                     /translation="MTKPALSLDGIRVIDLSRVIAGPWCGALLADLGADVIKVEDTGP
                     GDESRTWPPHKDGEAAAYLLFNRNKRGIALDLKTPEAVEVVKRLVKDADVVIENFRTG
                     TMESFGLGYDVLAQINPRLIYCSVSAFGRTGPRKDSPGYEALMQAFSGIMSITGEPGG
                     QPVRAGVSFLDLTTGILCALGVSNAIIQRAKTGLGQRVDGSLLETAVSLLAFHAEGYL
                     LTGALPRALGSGHPSLSPYRNFKCRDGQWIFIAAANDRFWQKLARALGLTDLAADPRF
                     EKNQGRVANRAELEGILEKTIGGLDREPLLKRLEEADVPATPVNTVDQVMNDPQTAER
                     GIVQRVTHPKLGEIPVVGTPLHFSRMSPGVRTAAPLRGEHTDTVLASLGYTPDEIKTL
                     RAKKAAI"
     gene            complement(7522..8325)
                     /locus_tag="VFX87_00270"
     CDS             complement(7522..8325)
                     /locus_tag="VFX87_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4993362.1"
                     /translation="MTAAAPASAAKPDVAPLGRMLLTQTWSELRIRWRIPAFSLTIVA
                     LPVLFFTFFGLPYAKQTLPNGTSLGAYLLASFAAYSVGSVMVFGFGIGVATERGLKVD
                     VLMRATPLPPYIAILAKVLNALFYALLSLVILIGYGIVVGGIRQDPTVWLDMIARLLA
                     GSVPFIALGFAIGYLCGPNVAPAVANLVYLPLTFASGLFMPLSQLPGFVRSIAPYLPA
                     YHYGQLGWSALGANSERLPVSLAWLAGYTLLFLAIAIRAYRNEESRKFG"
     gene            complement(8322..9332)
                     /locus_tag="VFX87_00275"
     CDS             complement(8322..9332)
                     /locus_tag="VFX87_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4993363.1"
                     /translation="MPPPSTRSATGATSPRSRSGRSSRTTRGGSTDSRGEPGASAPVA
                     LIGARKRYGDVEALRGIDLTIHAGEVVAMLGPNGAGKTTSISLMLGLRQPTAGQARLF
                     GLPPTDRRARSRCGVMLQESGITSVLTVREIVDVFRAYYPASLPAERAIAMAGLAEQA
                     SARIGTLSGGQRQRLYFALAICGDPDILFLDEPTVGMDVEARRAFTASIQTLSGAGKT
                     IVFTTHYLREAEELARRIIVIDRGAVIADASPQELKARVPGKKITLVAGRRFAPTDLD
                     GLPARVLSVSGERASLLSNEPAAVLRELFRRGVDVVDLEVTGADLEEAFLTLTRRAEG
                     AP"
     gene            complement(9226..>9772)
                     /locus_tag="VFX87_00280"
     CDS             complement(9226..>9772)
                     /locus_tag="VFX87_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4993364.1"
                     /translation="LPLYEEADRLDVMLGVHASGSHLGGAGVDLFPQFIQAHTCSHAF
                     GQMRQITSMIFEGIPERFPRLRIAYLEAGCGWAPYWAERMDDEYAKRASEAPALTRKP
                     SDYIRSGSIFFSCEADEWLLPQALKLIGENQVVYASDFPHWDNSYPASLDEIRDRGDI
                     TEIQKRKILSDNTRRLYGLKG"
BASE COUNT         1383 a         3467 c         3553 g         1369 t
ORIGIN      
        1 cgccgccggc ggctaccggg agctgcggct gacgtgaggc gctagcgcag ctcgcgggcg
       61 gccttctcca cgaaccgctc gaggccgccc gcgtcgagtg atcgcggcgg catcaggatc
      121 aggcggtgga ccccgagcgc ggcgaagcgg tccgcgctcg cgcggtccat cgcctcgccg
      181 ccccgggtcg gacgcggcgt gacgctgatc tcgagtgcgc cgagcgcggc gggccgctcg
      241 tgacgcgcgg ccgcctgacg gaggccttcg agacagcgct gcaccccggc ctcgtcgagg
      301 gcgaagccgt accagccgtg cccctgctcc accgctcgcc ggaacgcggc ctcggtgtgc
      361 ccgcccacca cgagcggcgg cccgggctgc tgggtgggcc gaggatgcgc ctgcaccccg
      421 tcgaagctca cgaagcggcc gcgatacgcc ggccgcgcct cgctccagat cacccgtagc
      481 gcggccaggt agtcgtcggt gaccgggccg cggtgctcga acgggatgcc gagcgcgcgg
      541 aactcgggct cgaggtagcc caccccgacg ccgaagatca ggcggccgcc ggagagcacg
      601 tcgagcgagg cgagctgctt ggccaggacg agcgggttgc gctgaggcag gatgatgatg
      661 ccggtgccga gccggatgcg tgtggtgtgc gcggccacga aggcgagcgt caccaccggg
      721 tccatgatgc ggtcctcggg ctccatcggc gacgggggca cgcgcgggtc cggcagcacc
      781 acgtgctcgc cgccccagag cgagtcgaag ccggccgctt ccgccgcgcg cgccacgcgg
      841 gccgcgccgt ccggataggt gcacggcccg gtggtcatcg agaagaggcc cagcttcagc
      901 ggcatcaggc gtccttgacg agcagggccc gcgcgcgctc gcgcaggacg tcgggcagcg
      961 gcgcgggccg gcgggcgtcc tgatcgaagt ggaccccgga ctgctcgagc gtggccacga
     1021 cctcgcccgc cggctcccgc gtcatcacgt ggaggagccg cagcgaggag ttgcccacgt
     1081 ggaggatgcc ggaccgcacc cgcacgccgt cgcccggatg cagggggctc ccgagctcga
     1141 gctggaactc gaaggtggag aagccgcggt gctccgcgcg catgtaggcc ggtgtcatgc
     1201 cgaaggcggc gatggcgtgg ccgttggccg ccgagaagcg gtggatgtag tgcgagagcg
     1261 cggcgccgcc cgagatgtcc acctccaccg gcccgacgat gtcgcgcgcg gtgtcccgga
     1321 agcccgcgag gccgggcggg cgcggccgtc gctcgcgggc tggtccgtcc cactccaccc
     1381 ggcgcgcctc cacggcccgt cgcgtctccg gggtcagggg cgcggcgagg cgcacgcgct
     1441 gctccaccgt ggtgcagagc acgcccgcgc cggagtcgaa gagcttgtgc cccagcacga
     1501 gcgcgtcgag ctccaccgag atcacgccgc tctccatgtg catgatgtcg cctacgcgca
     1561 gctcgcgctg gtagcggacg aaggaatcgg cggtgggggt ggccgcggcg gtcaggccga
     1621 ccgccgccag cagcccggcg cccgcgtcgg cgatgcgggc gaagtagtac gccaccgtca
     1681 ggtggtcgtt ggggtccacc tcccagcgga agacggtgcc ccggtaggtc tcgagccagt
     1741 cccccacggc cgacgcgggc ctagatgtac accgacttca tcgcgggcac cgggtagcgc
     1801 tcgcccgcgg cggcgccggc ggggatggcc tcctgcagcg cggcgacctc ctccggggtg
     1861 aggcgcacgt ccgcggcggc cacgttctcc tcgagccggt ccttccgctt ggtgcccggg
     1921 atcggcacga tgtccgggcc ccgcgcgagc agccaggcca gcgcgagctg cccgggcgtg
     1981 cagcccttct tccgggccat gtcgtcgagc ggcttcagaa gctcgaggtt cttcttgagg
     2041 ttctcctcct ggaagcgcgg gtggtcgcgc cgccggtcgt ccgaggggat ctccgcggcg
     2101 gagcgggtgc gcccggtcag caggctgcgg ccgagcggcg agtaggccat gaaggagatg
     2161 cccagctcgc gcagggtcgg gagcgtctcc tccgcctcca cgcggtagag cagggagaac
     2221 tcggactgca gggcggtgat cgggtacacc gcgtgcgcgc ggcgcaccgt cgccggcgac
     2281 gcctccgaca ggccgaggaa gcgtaccttc cccgcctcca ccaggcgcga catcgcgccc
     2341 accgtctcct cgatcggcac gctcgggtcc acccggtgct ggaggtagag gtcgatggtg
     2401 ctcacgccca ggcgcttcag gctcgcgtcg caggcctggg gcacgtactc ggggcggccg
     2461 ttgacccccg cggtcccgtc cggcttgcgg aggttgccga acttggtggc gagcaccacc
     2521 tggtcgcggc ggccgcgcag ggcccggccg agcagctcct cgttctggcc ccagccgtac
     2581 atatcggagg agtcgaggaa gttcacgccg agatcgagcg cgcggtggac gaccgcgatg
     2641 gactccgcct cgtcgcctcg gccgtagacg ttggacatgg acatgcagcc gaggccgatg
     2701 gcggaaacgg ccagcgagct cttgccgagt atgcgtcgct ccacgagttc ctcctcggaa
     2761 cctgccaaag ttggttagga tacggcgacc gattctatcc caagaccgag gtgcttgcga
     2821 cccgtgccgc acgacgtgac cctcctccgc gacatcgccc tcgggatcgt gttcgccgcg
     2881 accctggggc acgtggcccg gctgctccgg cagcccctgc tcctgggcta catcgcgggg
     2941 ggggtcctcc tgagctccca gatgggcttc gggctcatca ccaacgcgga gagcatcgag
     3001 ctgatctcgg agatcggcct gatcctcctc ctcttcatca tcgggctcga gatcgacctg
     3061 cgagagctcc agcgcctggg ccgctccatg ctggtcctgg gggtcggcca gttcgtgatc
     3121 aacgccctgc tgggcctggc cttcttcgcc tggctcggct accgggtggc ggagggccgc
     3181 ttcgacctga tctacctcgc gatcgtgatg tcgctgagct cgaccctgat cgtggtcaag
     3241 ctcttgcgcg agaagttcga gctcaagatc ctctccggac ggctcaccct gggcgtcctc
     3301 gtggtccagg acatctgggc catcctgttc atggcggtgc agccgagcct ggccgagccg
     3361 ggggcgctcc agatcgccca gtccgtcgcg ggcggcgcgg tgctcgtcgg cgtcgccttc
     3421 ctggccagcc ggtacatcct ggcgcccttg ctcgaggctt ccgcgcgccg gcccgagctg
     3481 gtgctgatct cctcggtggc gtggtgcttc ggcatcgccg gcgtcgcgca ccagctcggc
     3541 ctctcacgcg agatgggcgc cctgatcgcg ggagtgagca tctcggcctt cccctacggc
     3601 gccgacgtca tctccaaggt cgcgggggtg cgcgacttct tcgtcaccct cttcttcgtg
     3661 tcgctcggcc tgaaggtgcc ggtgccgagc ggggacgtcc tcggccacgc gctgctcatc
     3721 gtggtcttcg tgctggtgag ccgtgtcctc tcggtggtgc ccacctcgta cgtgctgggc
     3781 gacggcctct acgcgggcat ggtcaccgcg ctgaacctcg cccagatcag cgagttctcg
     3841 ctcgtgatct tgaccctggg cacgggctac ggccacgtct cggagcgggt gagcgcggtg
     3901 gtgctcaccg ccatgatcct gacctcgctg ctctcgccct acctgatcac cgggaacgac
     3961 cgcatcgccc ggtggctcct gcggcccttc gtgcgcggtc ccgcggcggg cagggccgcc
     4021 gcgcccaccg cggaggcgcg cgcgccgcgg gacatcgtgc tgctcggcca cttccgcatc
     4081 gcccaggcgg tgctggacct ggtggaggcg caggcgccgc agctcaagga gcgcatcacg
     4141 ctggtggact acgacgccaa ccgcgcccac ggggtcatgg cgcgcggctt ccgctgggag
     4201 tacggcgatc tcgccaatcc cgacgccctc cagcacctgg gcatcgagga ggcgcgcatc
     4261 gtggtgacca cgatctccga caccttcctc aagggcatca gcacgcgccg gctggtgggc
     4321 aacctgcgcc gcctcgcccc gcgcgcggtg atcgtgatga ccggcgagga gcggaccgac
     4381 atggaggacc tgctgcgcgc gggcgccgac cacgtgctga tcccgggcga gatcaccggg
     4441 gagcgcatcc tcgagctgct ccgcaagggc gacgccgcct agcgccggcc gatcaggcga
     4501 tgccggtcat gcgcgcgaag gcggtcagct cacgcgggat gtccccgccc gagggctggt
     4561 aggcgatctc ggtcactccg cgcgcggcga gctcggcgac ccgcgcgcgc agcgccgtcg
     4621 cctcgccggt gaaggtgacg cgccggatca ggtccccgct caccaccgcg cggtccaccg
     4681 ggttcagctc ggtgagatgg ccggtgtgcg tgcgcaggtg gcgctcgcgc gcgggcaccg
     4741 cctcgatggc ggcctgccac gcgggcccgc cgggcagcgt ggccagatcg aggccgggca
     4801 cgttgcggta ctcgtaggcg cggtggagca gcacggccgc ccccgccccc gccgcgtcga
     4861 gcacccgcgg cgcgtcgggc cgctcgccgg gctccagcac cgtgccgagg gtgagcagga
     4921 tggaccaggc aaagcccggc gtgggcacca ccgagaagac accgtcgccc tccgcgtggg
     4981 ccaccgcctc gcctcgtggg ccgcgcgtgc cgaagacgaa cggcacacgg atcggccggc
     5041 gcggggcctg cccggcaccg tgcaggagct gggtgagggc gccgtccacc tccaccatct
     5101 cccccgcgag caggccgcgg agctggcgcg cgtagagcgc gaccgatgtc cacgagttgg
     5161 ggcgctggcc catggccatg cgtccggtga agccggagcc gatgcccacg ttcacgcgac
     5221 cgggtgcgat gtccaccagc gtggcgaccg cggcggcggt caccaccacg tggcgcaggc
     5281 tcgggatcag cacgccgggg cccagctcga tgcgccgggt gcgctccgcg gcgcggccga
     5341 gcgtcatcca cacgtcgagc tgcagcgcgg gcgtgtcgta gacccacgcg cgccggtagc
     5401 cgagccgctc cgcgagcgcg atgtgctgcg gagtgcccgg caccggcggg aacgcgcagg
     5461 agatgtccac tgcggggcgg accctcaggc cgggcggccg gggccgagcg gcgcgccgcc
     5521 ccagcgctcg agcatcgcga actccccgac cggcttgcgg gtgagcttgg tgatgggctt
     5581 gaccggccgg ccgagtggca tcaccgccgc caccgccacg tgggagggca ggccgagcag
     5641 cttctggagc gcgggctcct gggcggtggc cagcgtggtg atggtcccgg cgaagccctc
     5701 gtggcgcgcg gcgagcagga tgttccacgc gaacggatag atggacgccc cgctgatcac
     5761 gccgacgcgt ttcaactcgg agtccatcga ggccacgacc ttcaggtcca cgcagacgac
     5821 gaggaccacc ggcgccttga gcaccggctc gatcagccgg ggcggcgcgg gggtgcgctc
     5881 gatggtggcc gcgtccacct tggtggggtc gatggtgttc caggggctct cgcccacctg
     5941 cacctgcgcg gcgtagcgct tggccgcggg aacggtcgcg acggccagcc cctcgcgcgt
     6001 cttcggctcg cgcaccacga tcacgcgcca gccctgacgg ttgccgccgc tcggggcgaa
     6061 gcgcgcgcgg tccaggatct tgaagaggat ctcgtcgggc agcgggtcgg cggtgaactc
     6121 gcgggccgag aacgtcgtgc gcatcacgtc gtagagctcc atgggcggac cccctgagga
     6181 tggattggcc gaggggtgaa tatacaccgc caacggcggt atagtacgcg ccatgaccaa
     6241 gcccgccctc tcgctcgacg gcatccgcgt catcgatctc tcccgcgtca tcgccgggcc
     6301 ctggtgcggg gcgctgctgg ccgatctcgg ggccgacgtc atcaaggtgg aggacaccgg
     6361 gcccggcgac gagtcgcgca cctggccgcc ccacaaggac ggcgaggccg ccgcgtacct
     6421 gctcttcaac cggaacaagc gcgggatcgc gctcgacctg aagacgccgg aggcggtgga
     6481 ggtggtcaag cggctcgtga aggacgcgga cgtggtcatc gagaacttcc gcaccgggac
     6541 catggagtcg ttcggcctcg gctacgacgt gctggcccag atcaacccgc ggctcatcta
     6601 ctgctcggtc tccgcgttcg ggcgcaccgg gccgcgcaag gacagcccgg gctacgaggc
     6661 gctgatgcag gccttcagcg gcatcatgtc catcaccggc gagccgggcg gccagccggt
     6721 gcgggcgggc gtgtccttcc tcgatctgac caccggcatc ctctgcgcgc tcggcgtctc
     6781 caacgcgatc atccagcgcg cgaagaccgg gctcggccag cgggtggacg gctcgctcct
     6841 cgagaccgcg gtgagcctgc tcgccttcca cgccgagggc tatctgctga ccggcgcgct
     6901 gccgcgcgcg ctcggctcgg ggcacccgtc gctctcgccc tatcgcaact tcaagtgccg
     6961 cgacgggcag tggatcttca tcgccgccgc caacgatcgc ttctggcaga agctcgcccg
     7021 agccctgggc ctgaccgacc tggccgccga cccgcgcttc gagaagaacc aggggcgggt
     7081 ggccaaccgc gcggagctcg agggcatcct cgagaagacc atcggcgggc tcgaccgcga
     7141 gcccttgctc aagcgcctgg aggaggccga cgtccccgcc acgccggtga acacggtgga
     7201 ccaggtcatg aacgacccgc agaccgccga gcgcggcatc gtgcagcgcg tgacgcatcc
     7261 caagctcggc gagatcccgg tggtcggcac cccgctccac ttctcgcgca tgtcccccgg
     7321 cgtccgcacc gccgccccgc tccgcggcga gcacaccgac accgtcctcg cctccctcgg
     7381 ctacaccccg gacgagatca agaccctccg cgccaagaaa gcggcgattt agcctggggt
     7441 caggtcttac attccgacac tcttcgcggc cggccagtcg cggggcgcga gaaatgtcgg
     7501 aatgtaagac ctgaccccga gctagccgaa cttgcgggat tcttcgttgc ggtaggcgcg
     7561 gatcgcgatc gcgagaaaga gcagggtgta gccggcgagc caggcgaggg aaaccggaag
     7621 gcgctcgctg ttggcgccca gtgcgctcca gccgagctgg ccgtagtggt aggcggggag
     7681 atagggcgcg atcgagcgca cgaagccggg gagctgagag agcggcatga agagccccga
     7741 ggcgaacgtc agggggaggt agacgaggtt cgcgaccgcg ggcgccacgt tggggccgca
     7801 tagatagccg atggcgaagc ccagcgcgat gaacggcacc gagcccgcga gcaggcgcgc
     7861 gatcatgtca agccacaccg tgggatcctg gcggatcccc cccaccacga tgccgtagcc
     7921 gatcaggatc accagcgaga gcagcgcgta gaacagcgcg ttcagcacct tggccaggat
     7981 cgcgatgtag ggcggcagcg gggtcgcgcg catcagcacg tccaccttca ggccgcgctc
     8041 ggtggccacc ccgatgccga agccgaacac catcacgctc cccacgctgt aggccgcgaa
     8101 cgaggcgagc agataggcgc cgaggctcgt gccgttgggc agggtctgct tcgcgtaggg
     8161 cagcccgaag aaggtgaaga agagcacggg cagcgccacg atcgtcaggc tgaaggcggg
     8221 aatgcgccag cggatgcgca gctcgctcca cgtctgcgtc agcagcatgc gcccgagcgg
     8281 cgcgacgtcc ggcttcgcgg cggacgccgg ggcggcggcg gtcacggcgc gccctcggcc
     8341 cgccgggtca gcgtgaggaa cgcctcctcc aggtctgccc cggtcacctc gaggtccacc
     8401 acgtccacgc cccggcggaa cagctcgcgc agcaccgccg ccggctcgtt gctgagcagg
     8461 ctcgcgcgct cgccgctcac gctcagcacg cgggcgggca ggccgtcgag atccgtgggc
     8521 gcgaaccgcc ggcccgcgac cagggtgatc ttcttgccgg gcacgcgcgc cttcagctcc
     8581 tgcgggctcg cgtccgcgat gaccgcgccg cggtcgatca cgatgatccg ccgggcaagc
     8641 tcctcggcct cgcgcaggta gtgggtggtg aagacgatgg tcttgcccgc cccggagagc
     8701 gtctggatgc tggccgtgaa ggcgcgtcgc gcctccacgt ccatgcccac ggtgggctcg
     8761 tccaggaaca ggatgtcggg atccccgcag atcgcgagcg caaagtagag gcgctggcgc
     8821 tggccgcccg agagcgtgcc gatgcgcgcg gacgcctgct cggcgagtcc ggccatcgcg
     8881 atcgcgcgct cggcgggcag cgacgccggg tagtaggcgc ggaagacgtc cacgatctcg
     8941 cggacggtca gcacgctggt gatgcccgac tcctgcagca tcacgccgca gcggctgcgc
     9001 gcgcgccggt cggtgggcgg caggccgaag aggcgcgcct ggcccgcggt gggctggcgc
     9061 agccccagca tcaggctgat ggacgtggtc ttgcccgcgc cgttcggccc gagcatcgcg
     9121 acgacctcgc ccgcgtgaat ggtgaggtcg atcccgcgga gcgcctcgac gtccccgtag
     9181 cgcttgcgcg ccccgatcag cgcgacggga gccgaggcgc cgggctcacc ccttgagtcc
     9241 gtagagcctc cgcgtgttgt ccgagaggat cttccgcttc tggatctcgg tgatgtcgcc
     9301 ccggtcgcgg atctcgtcga gggaggcggg atagctgttg tcccagtgcg ggaagtcgga
     9361 ggcgtagacg acctggttct cgccgatgag cttgagcgcc tgcgggagca gccactcgtc
     9421 ggcctcgcag gagaagaaga tggacccgct ccggatgtag tcgctcggct tgcgggtgag
     9481 cgcgggcgcc tccgaggcgc gcttggcgta ctcgtcgtcc atccgctccg cccagtaggg
     9541 ggcccagccg catccggctt cgagataggc gatccggagg cgggggaagc gctccgggat
     9601 gccctcgaag atcatcgagg tgatctgccg catctgcccg aacgcgtgcg agcaggtgtg
     9661 ggcctggatg aactggggga agagatccac gccggcgccg cccaggtgcg agcccgaggc
     9721 gtggacgccc agcatcacgt cgaggcggtc cgcttcctcg tagagcggca gg
//
LOCUS       DASUFA010000009        15265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_344941, whole genome shotgun sequence.
ACCESSION   DASUFA010000009 DASUFA010000000
VERSION     DASUFA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 15265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15265
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344941"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFX87_00285"
     CDS             <1..1007
                     /locus_tag="VFX87_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4993365.1"
                     /translation="WVALKCVGLTLVTLARSVGRTAAVLLAGRVHLRRAIEALAIDIG
                     VSIWARQLFTLLVSADLYRGVPAIYVNFLDYDVFSHAFGPADRLAFRSLRRVDRSIQQ
                     LARVIHRLPEMGYDLYILSDHGQAPTRPFRRVTGGVPIDRVVRDTLGVEGDEVKVIAA
                     GPNAFVYFLDRREALSAVEIESRHPGVLARLSRHRGIGLVLARGAGGPLCWHRGHRIP
                     LGRRGDPTVRDPFARRADRAVVLAGLRDLVAMPSAGDIVLYGTGAPGGDVSFIDERGA
                     HAGPSEEELHTFILHTPSVALPGGPLTHPVELYPHFRAYRDAGAGEPATTRSPACAAR
                     "
     gene            1351..1986
                     /locus_tag="VFX87_00290"
     CDS             1351..1986
                     /locus_tag="VFX87_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993366.1"
                     /translation="MDRAGERVRVEEITTVSYGNGRAMVGPWPLSWSAVWVGTLSAVA
                     LALVFGLVAITLGAHRVGQPFGTWREVSFPALVFSVFGSFLAFAAGGWVAAKVAGMRH
                     SESAMLHGAVAWLVAVPILVILASMGAVSYFGGWYGGLAGLPPAAAAQAFAAAQDPAA
                     VMAARNAALAALTALLLGLMGGVVGGWMGSGEPMTFTHYRTRRERAAAGGW"
     gene            2071..2412
                     /locus_tag="VFX87_00295"
     CDS             2071..2412
                     /locus_tag="VFX87_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993367.1"
                     /translation="MNEMMGDFVRLGLALTLFGFAAWVGGASVTAWVAWEKGREPVAW
                     FVLAFFLSPLLALVALSAVPARRLSRRREELPARPRPHPLEDPAIVAERLVNGLPRED
                     KRWATTTKRPW"
     gene            2382..2600
                     /locus_tag="VFX87_00300"
     CDS             2382..2600
                     /locus_tag="VFX87_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993368.1"
                     /translation="MGNDYEKTMVITAPGRTGTFDSFECALHAMAPTCEHCACRIIRH
                     GVEAPDGRYFCCAHCAEIAGVPGRKVNA"
     gene            2604..3563
                     /locus_tag="VFX87_00305"
     CDS             2604..3563
                     /locus_tag="VFX87_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4993369.1"
                     /translation="MADARRLWGVVLAGGEGVRLRPLVQRMLGEERPKQFVKLLGPRS
                     LLRQTLDRAALSISPDRTLVVTMRLHTDYVAEEFTGAAWPPYVLAQPEDRGTAAAILY
                     AAQWIAWREPAATIAVLPSDHFILGEATFMAHVREVAGALDRHPDRVVLLGAPPTSPE
                     DEYGWIEPGAALAGDEISAVRQFWEKPSEDRAQACLSAGCLWNTAIAVARVRAMLELG
                     ARALPEMSGRLAGLQRVRDSEQEMAALQEAYTLMPRASFSRAVLEPHLEHLGVSRLPR
                     VTWCDLGSPRRVLHVLARMRVRPAWADRVDVPAPPARVAMAPR"
     gene            3577..4413
                     /locus_tag="VFX87_00310"
     CDS             3577..4413
                     /locus_tag="VFX87_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX4993370.1"
                     /translation="MPSTIWTGSISLGLVNIPVSLVTAEQSDELDFTLLDKRDSSPIG
                     YQKISKRTGKPVPGERIVRGIEQTKGRYVIVNDADLRRASPERTQRIDILAFVEGAAI
                     PPLYFDRPYYLEPARGGERGYALFREALQRSGKVAVASVVVKTRQHLAAVLVHDRVLV
                     LDILRYATELRDSSKLRVPGAALRAMRISDRELKMAERLVKEMTADWDPGQYHDEYRD
                     ELLAFIKKRGRTGQLTSAPEAPEEPVTRKRADIIDMAELLKRSLARTGEDRRRPRRRK
                     SA"
     gene            4509..4670
                     /locus_tag="VFX87_00315"
     CDS             4509..4670
                     /locus_tag="VFX87_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4993371.1"
                     /translation="MIQRHAASHLHYDFRLELDGVLKSWAVPKEPAPVPSPERLAVHV
                     EGHPIEYGS"
     gene            4835..5044
                     /locus_tag="VFX87_00320"
     CDS             4835..5044
                     /locus_tag="VFX87_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993372.1"
                     /translation="MLHDEVEQLGHENERLRAELGRLDQLQAVIARVNQDNDRLRVER
                     DELLASFRRVASLVEQIQRARGAKE"
     gene            5098..6249
                     /locus_tag="VFX87_00325"
     CDS             5098..6249
                     /locus_tag="VFX87_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4993373.1"
                     /translation="MALIDKSEFVGLDGVVHLCTGGEAPWLRAHTAACERFGALKSAG
                     MAGREEMFAIGERARARAARVLGVASGEVAFLAHSSEGLNQAVRSVEWRAGDNVVFAD
                     VEYPSLIYPAALLRERGVEPRVVRARGHYVAMDDLAAQVDRRTRLLLVSQVSYLTGQR
                     LDLARCAELARGAGARLAVDATHAAGVVPVPGALCDFVVSSCYKWLLATHGVGLFAYS
                     ARRVGELQPATVGWHSVGHRGGPGDPLAMSWRPDASRLEAGNPSLLGLAVLDSALARL
                     ERLAPADVERHAQGLGTELIEGLKARGWPVTTPEAPEERGGNVCFLAEDAGGLAAKLA
                     AERVLVWGGEGRIRVSPHVHDDRDDIARFFEALDRVGARPQPSRSFIPS"
     gene            complement(6219..6595)
                     /locus_tag="VFX87_00330"
                     /pseudo
     CDS             complement(6219..6595)
                     /locus_tag="VFX87_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(6588..7490)
                     /locus_tag="VFX87_00335"
     CDS             complement(6588..7490)
                     /locus_tag="VFX87_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006095262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX4993374.1"
                     /translation="MPARVLHFDAFAPRPGMGNPAGVVLDADGLSDATMQAIARAVGF
                     NETAFVLPSTRADLRLRYFTPGHEVDLCGHATVGSVVALHQHRRLPGAGFPRRLTLET
                     GAGRLPVTVELGGAGDPVVIMAQAPAQFESFAGDRAALARVLGIGPDDLRADLPILYG
                     STGLWTLVVPVHGLGAMRRMKPLTALFPSVLTERPGASIHPFCVETIHRDTHLHARHF
                     SSPRSGTVEDPVTGTAAGVLGAYHLEFIGDDTPSPLLVEQGQEIGRDGRVRVWVERRD
                     GRCHVRVGGTACFVRELAIEVAHA"
     gene            complement(7506..7748)
                     /locus_tag="VFX87_00340"
     CDS             complement(7506..7748)
                     /locus_tag="VFX87_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX4993375.1"
                     /translation="MLELRPGCECCDRDLPPGSEDARICSFECTFCRACAETVLHGRC
                     PNCGGELVPRPRRPADKLAKFPAAAARVHKPAGCRS"
     gene            7801..8544
                     /locus_tag="VFX87_00345"
     CDS             7801..8544
                     /locus_tag="VFX87_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4993376.1"
                     /translation="MAKSVVTPERFAKGMTFDEYMKYTGSAENLDREAFGSYFPDGGA
                     KPAPRKDNSAVFRERYARARLSDHQVSAIRWLAAQPGGPAKILVISEDWSSDCRRDLP
                     WLARLAEAGGLELRIFNRDGRKVLGTRRPDPSVAPDGNHDIMLEFLNAKSGAEWASVP
                     VVVVYTKDLQELHRYIEYPAIYHKDPVRAHQQAARDGETAEQGRERGMREFAAMQAGP
                     FFDLWAAACVDEILSALYEKRILATTSAG"
     gene            complement(8533..8904)
                     /locus_tag="VFX87_00350"
     CDS             complement(8533..8904)
                     /locus_tag="VFX87_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4993377.1"
                     /translation="MPIVGVDHVQLAMPAGREAEARAFYAGLLGIPELAKPPALAARG
                     GAWFERGPVKVHLGVDPDFRPARKAHAGLLVSGLDALIEELRRAGYDVTTDEPLEGYL
                     RVYVNDPFGNRLELMEPRSAR"
     gene            complement(8925..9665)
                     /locus_tag="VFX87_00355"
     CDS             complement(8925..9665)
                     /locus_tag="VFX87_00355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4993378.1"
                     /translation="MSRWLGRLALPLAAAVLLGASSPAIAQRTPRFLFPALAISGGAL
                     LVLSTVRAGRDLRRGARRPDVGLAVTAVATLLFAVLPFARFVYMIAPAAGGAPRILTG
                     FGAWRGAEGYPLLDPHRGIDIKGRVGTDVLAAAEGRVTVARERQDLCGLIVVVVHEPH
                     GYRTVYCHLAETAVKVGENVARGQRLGALGTSGQRAWPGYEHVHLELQRGVDPEDLHD
                     PLPRIVGCFDATARYPADRLALTYPVPC"
     gene            complement(9708..10085)
                     /locus_tag="VFX87_00360"
     CDS             complement(9708..10085)
                     /locus_tag="VFX87_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4993379.1"
                     /translation="MTAGAAPRKERFDEARAVLARTYAAFNARDIDTVLGVMHPDVIW
                     PNGMEGGTVVGHAAVRAYWTRQWALIDPRVEPTGFTVEADGRVAVAVHQVVRDLTGTV
                     LKDAMVEHVYAFEGGLITSMEIR"
     gene            complement(10082..10882)
                     /locus_tag="VFX87_00365"
     CDS             complement(10082..10882)
                     /locus_tag="VFX87_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4993380.1"
                     /translation="MPHVSIGDAEIYYEEEGRGPALMLVPGLGGQGAFWGPQVRDFAR
                     DFRVIVHDHRGAGRSTHSRIQYSVDQMAADTIHLMDRLGVEAAHYVGHSTGGAIGQTI
                     AQDHPGRIRSLVLSATWAGQDAYFRRCFETRKELLLHRGFESYWRASVVMLRPPRWIS
                     ENDALLEEEMKRMGAAPPPVEVLASRIDAIVRFDRRARLGEIRCPTLVVVAADDALTP
                     AFYSEELAARIPGAKLAVLRTGGHFAPALVPEEYNEVVGAFLRDQLRS"
     gene            complement(10891..11400)
                     /locus_tag="VFX87_00370"
     CDS             complement(10891..11400)
                     /locus_tag="VFX87_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993381.1"
                     /translation="MSQTASQVDQVRGRAVVMQPEDGPSYWQPVPANGHADPKLYPAR
                     TGFAGHSMGFQTIAPGSRVREHSHGDQVELQICFRGQGRVVVDGQSHPLVPGTACFLG
                     YDVRHELINESAQDDLVMLWVITPPGLENFFEAIGRPREAGAPAPAPFERPTDVIAIE
                     RRMGMNDTK"
     gene            11490..13040
                     /locus_tag="VFX87_00375"
     CDS             11490..13040
                     /locus_tag="VFX87_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993382.1"
                     /translation="MKLTRALAISAVLLATILSGPAPAQDAPRPGGVLKAAMIGEPPT
                     LDLHWTTAVITQEIAFHLYEGLFTYDAKFAPIPMLAESYTMMPDGRRYTFVLRKGVRF
                     HTGKEMTSVDVVASLTRWGKVATTGKAVWRAVEAIEAKGPHTVVIHLKEPSGSLLYAL
                     GSPNNGASIHPKEIIDAPGDGQIKEFVGTGPYRFVEHRPDRHIKVARFKDYAARREAP
                     DGYGGRRAAYVDEILFIPVPDVAVRLAGVETGEYHFANTINPDKYEGIKGRADITAAA
                     VKPYGWVTAVPNHKQGVMANKKVRQALQAVLDMEPIMAAGIGNKDFYRLSGTLFYPEQ
                     AAFYSTTGVTGYNLKNKDRARALLKEAGYAGQPVRWVTTKEYEWMYNTAVVAKQQMEE
                     AGFVVDLQVVDWATLVQRRNKPELFDLFSTGFTFNADPALASSLQCNWPGWWCAEEKD
                     KLMADLTRETDPRKRRAIIERLQAIFYEDVGRIKLGDYYQLQAMRKDLRGFKPGPYLY
                     FWSAWLAK"
     gene            complement(13149..14738)
                     /locus_tag="VFX87_00380"
     CDS             complement(13149..14738)
                     /locus_tag="VFX87_00380"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX4993383.1"
                     /translation="MLDIKITGGQVIDGTGAPARRADVGLTGDRVTTVGDLSAEPAGR
                     TIDATGRTVTPGFIDMHSHSDWRLWDNRRAESKIRQGVTTEVVGNCGFSPAPVSDAFR
                     DEMKGFALYLPAGMDFSWTSVREFLARYEDEGIALNVAQLIGHGTLRLAAMGFARRPP
                     TAAEQAAMERMMDEAMADGAYGLATGLIYAPGSYAATEEIIGIARRAGGRRGFYASHI
                     RGEGATLLDAVAEAIRVGREGGLPTQVSHVKAAGRPNWGRVADALALIDAARARGQDV
                     MGDVYPYTASSTTLRTLLPDWVLEGGIDAMLKRLADRATRERIRKDLTGGGETLLRGL
                     DWADIMVAYSPSRPDAQGRRIAEIAAARGEDPLDAAIELIVAEQGKGYMILFQLDEAD
                     LRRTLTHPHVMIGSDGSSLAADGPLATGKPHPRSYGTFPRVLGRYARDERVLSLPDAV
                     WKMTGMPARRLGLSDRGVLAPGAKADVVVFDAATVADRATYEDPHRYATGIGHVLVNG
                     RVVIADGEHTGALPGRVLRPS"
     gene            complement(14744..>15265)
                     /locus_tag="VFX87_00385"
     CDS             complement(14744..>15265)
                     /locus_tag="VFX87_00385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX4993384.1"
                     /translation="FQAALSWDQVKRFKDRHDLPLILKGIATGEDAAIACDHGVDAVY
                     VSNHGGRQLDHGRGSIEVLPEVVKAVAGRSVVMVDGGFMRGTDVVKAIALGAQMVGIG
                     RLSCLGLAAAGEPGLVRALELLEEEIRIAMGLLGVHSLAQLDSSYLHPATPVTLPHVT
                     SAFPLLDLDDRRY"
BASE COUNT         2158 a         5335 c         5593 g         2179 t
ORIGIN      
        1 gctgggtggc gctcaagtgc gtgggcctca ccctcgtgac gctcgcgcgc tcggtgggac
       61 ggaccgcggc cgtgctcctc gccgggcgcg tccacctccg ccgcgccatc gaagcgttgg
      121 cgatagacat cggcgtgtcc atctgggcgc ggcagctctt cacgctgctc gtctccgcgg
      181 atctctatcg cggggtgccc gcgatctacg tgaacttcct cgactacgac gtgttctcgc
      241 acgccttcgg cccggcggat cggctcgcct tccgctcgct gcggcgcgtg gaccgctcga
      301 tccagcagct ggcgcgcgtc atccaccggc tccccgagat gggatacgac ctctacatcc
      361 tctcggatca cgggcaggcg cccacgcggc ctttccggcg cgtcacgggg ggcgtgccga
      421 tcgaccgggt ggtgcgcgac accctcggcg tggagggcga tgaggtcaag gtgatcgcgg
      481 ccggccccaa cgccttcgtc tatttcctgg accgccgcga ggccctgtcg gcggtggaga
      541 tcgaatcgcg ccatccgggc gtcctcgcgc gcctctcgcg ccaccggggc atcggcctcg
      601 tgctcgcgcg cggcgccggc gggccgctct gctggcaccg cggccaccgc atcccgctgg
      661 gccgccgggg cgatccgacc gtacgcgatc ccttcgcgcg acgcgccgat cgcgcggtgg
      721 tgctggcggg gctgcgcgat ctggtggcga tgccgagcgc aggggacatc gtgctctacg
      781 gcacgggcgc ccccggcggc gacgtgagct tcatcgacga gcgcggcgcc cacgccggcc
      841 cgtcggagga ggagctgcac accttcattc tgcatacgcc atcggtggcg ctgcccgggg
      901 gcccgctcac gcatccggtc gagctctatc cccacttccg cgcctatcgc gacgcggggg
      961 ccggcgagcc ggcgacgacc aggagcccgg catgcgcggc ccgctgaccc cgcggcccgt
     1021 cctgtcgctg cgcgcgtacc tcccgggctc gtcgtggtgt gcgtggtgcc ggtgctggcg
     1081 ttctccgtgt ggctgaccct cgtcctcacc gcgctcggcc catgctcgat cggccgggga
     1141 tgtagttgct cggcgcgctc ggtgtcgcga tggccgaggg tcggacgttg agccccttcc
     1201 gagggtccgc gccgttctta ccgggtaaac cgttcccatg ttttctcgcg cggctggacg
     1261 tcggcgcgcg cgagcgcgag ttgcgtggtt catcccacga tcggaggccg ggcacagaag
     1321 ttgctctttc cccggccggg aggacacgag atggacaggg cgggggagcg ggtcagggta
     1381 gaagagatca ccacggtgag ctacgggaat ggcagggcga tggtaggacc ctggccattg
     1441 tcgtggagcg ccgtgtgggt cgggacactg tctgcggttg cgctggccct cgttttcggg
     1501 ctcgtcgcca tcacgctcgg cgcacatcga gtcggtcagc cgttcggcac ctggcgcgag
     1561 gtgagcttcc cggcgctggt cttcagcgtc ttcggctctt tcctggcgtt cgcggccggc
     1621 ggctgggtcg cggccaaggt cgcgggcatg cggcactccg agagcgcgat gctgcacggg
     1681 gcggtcgcgt ggctcgtggc ggtgccgatc ctggtgatct tggcctcgat gggcgcggtg
     1741 tcctacttcg gcggctggta cggcgggctc gcgggccttc cgccggctgc ggccgcgcaa
     1801 gccttcgcgg ccgcgcagga tccggcggcg gtgatggcgg cgcgcaacgc cgccctggcc
     1861 gcgctgaccg ccctgctgct gggcttgatg ggcggggttg tcggcggctg gatgggctct
     1921 ggtgagccaa tgaccttcac acactatcgg acgcggcgcg aaagggccgc ggcgggaggc
     1981 tggtaatgcc actcattctg ctctggctcc tcggcgtgcc ggtgagcctc attctgatcc
     2041 tgttcctgct gggtgtcgga cgttagggcc atgaacgaga tgatgggcga cttcgtgaga
     2101 ctgggactgg cgctcacgct gttcgggttc gcggcctggg tgggcggggc atccgtgacc
     2161 gcctgggtcg cgtgggaaaa gggccgcgaa cccgtcgcct ggttcgtgct ggcgttcttt
     2221 ctctccccgc tgttggccct cgttgcgctg agcgcggtgc ccgcgcgacg cctgtcccgc
     2281 cggcgggaag agctgccggc gcgcccccgg ccccatccac tcgaagaccc cgcgatcgtg
     2341 gcggagcgcc tcgtgaacgg gctgccccgc gaggacaagc gatgggcaac gactacgaaa
     2401 agaccatggt gatcacggcc cccggccgca cgggcacgtt cgactccttc gagtgcgccc
     2461 tgcacgcgat ggcccccacc tgcgagcact gcgcctgccg gatcatccgc cacggagtcg
     2521 aggcgcccga cggccgctac ttctgctgcg cgcattgcgc ggagatcgcc ggcgtgcccg
     2581 gacgcaaggt caacgcgtag cccgtggcgg acgcccggag gctctggggg gtcgtgctgg
     2641 cgggcgggga gggggtgcgg ctacgccccc tcgtccagcg catgctcggc gaggagcggc
     2701 cgaagcagtt cgtcaagctc ctcgggccgc ggagcctgct gcggcagacg ctggatcggg
     2761 cggcgctcag catctcgccg gaccgcacgc tggtggtgac gatgcgcctg cacaccgact
     2821 acgtcgccga ggagttcacg ggagccgcat ggccgccgta tgtcctggcc cagccggaag
     2881 accggggcac cgcggccgcc atcttgtacg ccgcgcagtg gatcgcctgg cgcgagccgg
     2941 ccgcgacgat cgcggtcctt ccgtccgatc acttcatcct cggcgaggcg accttcatgg
     3001 cccacgtccg ggaagtggcg ggtgcgctcg atcggcatcc cgaccgtgtg gtcttgctgg
     3061 gcgcgccgcc cacgtcgccc gaggacgaat acggctggat cgagcccggt gcggcccttg
     3121 ccggcgacga gatctcggcg gtgcggcagt tctgggagaa gccgtcggag gaccgcgcgc
     3181 aggcgtgcct gtccgccggc tgtctctgga acaccgccat cgccgtcgca cgggtgcgcg
     3241 cgatgctcga gctcggtgcg cgggcgctgc ccgagatgag tggccggctg gccggactcc
     3301 agcgcgttcg ggactcggag caggaaatgg cggcgctaca agaggcctat acgctcatgc
     3361 ctcgcgcgag cttctcgcgg gccgtcctgg agccccatct cgagcacctc ggcgtctctc
     3421 gtctgccccg ggtgacctgg tgcgacctgg gcagcccccg ccgggtcctc cacgtgctcg
     3481 cccgcatgcg ggtgcgaccg gcgtgggccg accgggtgga cgtgccggcc ccgcctgctc
     3541 gcgtggccat ggccccccgc tgagcggagg cgcggcgtgc cgtcgaccat ctggaccggc
     3601 agcatcagcc ttggtctcgt caacatcccg gtgtccctgg tgacggccga gcagagcgac
     3661 gagctggact tcaccctgct cgacaagcgg gacagctcac cgatcgggta tcagaagatc
     3721 agcaagcgca ccggcaagcc ggtgccgggc gagcggatcg tgcggggcat cgagcagacg
     3781 aaggggcgct acgtgatcgt caacgatgcc gatctacgcc gcgccagccc cgagcgcacc
     3841 cagcgcatcg acatcctcgc cttcgtggag ggagccgcga tcccgccgct ctacttcgat
     3901 cggccgtact atctcgagcc cgcccgcggc ggggagcgcg gctacgcgct gttccgcgag
     3961 gccctgcagc gcagcggcaa ggtcgcggtg gcctcggtgg tggtgaagac gcgccagcat
     4021 ctcgccgcgg tgctcgtgca cgaccgggtc ctggtgctgg acatcctccg atacgccacg
     4081 gagctgcgcg attcctcgaa gctccgcgtg cccggggccg cgctgagggc catgcggatc
     4141 tcggatcggg agctgaagat ggccgagcgg ctggtgaagg agatgaccgc ggactgggac
     4201 ccggggcaat accacgacga gtaccgggac gagctgctgg ccttcatcaa gaagcgcggc
     4261 cggacgggcc agctgacctc ggcccccgag gctcccgagg agccggtgac acggaaacgg
     4321 gccgacatta tcgacatggc ggagctgctc aagcggagtc tcgccaggac gggcgaggac
     4381 cggcgccggc cgcggcgacg caagtccgcc tgagccgtga gcctcgcgct ctaccgtcgc
     4441 aagcaggatt cgcccggacg ccggagccca gggggcggcc gcgccggcct gcgaagcgct
     4501 tgtcgttcgt gatccagcgc cacgccgcga gccatctgca ctatgatttc cggctcgagc
     4561 tcgacggcgt gctgaagagc tgggcggtgc cgaaggaacc ggcgcccgtg ccgagcccgg
     4621 agcgcttggc ggtgcacgtg gagggtcatc ccatcgagta cgggagttag ccttgcccgc
     4681 gggccttccg gtcacgctat catccccttg atgaccgccg cccgcgaacg agcgacaagc
     4741 cccgatgtgc acgcgaggct gagggcccgg ccgtatgaac cctgagcgag aggacactgc
     4801 ccgctggcta gaagaaggcc gtgagctcct cggcatgttg cacgacgagg ttgaacagct
     4861 gggccacgag aacgagcggc tgcgcgccga actcggacgg ttggatcagc tgcaggccgt
     4921 gatcgcgcgc gtcaaccagg acaacgaccg gctgcgggtc gaacgagatg agctattggc
     4981 gagcttccgc cgcgtcgcta gtctggtgga acagattcag cgcgcgcgcg gcgccaagga
     5041 gtagtgcccg cccggacggc cgctccgcag cgctacccgg cacaggagga acggatcgtg
     5101 gccctgatcg acaagagcga gttcgtcggc ctcgacggcg tggtccatct ctgcaccggc
     5161 ggcgaggcgc cgtggctgcg ggcccatacg gccgcctgcg agcggttcgg cgcgctgaag
     5221 agcgccggca tggccgggcg cgaggagatg ttcgccatcg gcgagcgggc gcgcgcccgc
     5281 gccgcgcgag tgctgggcgt ggcgtccggc gaggtcgcct tcctcgccca ttcgtcggag
     5341 ggcctgaacc aggcggtgcg ctcggtggag tggcgcgcgg gcgacaacgt ggtcttcgcc
     5401 gacgtcgagt acccctcgct gatctacccg gcggcgctcc tgcgcgagcg gggcgtggag
     5461 ccccgcgtgg tccgggcccg tggccactac gtggcgatgg acgatctggc cgcgcaggtg
     5521 gaccgacgga cgcggctctt gctggtcagc caggtgagct atctcaccgg ccagcgcctg
     5581 gacctggccc gctgcgcgga gctggcgcgc ggcgcgggcg cgcggctcgc ggtggacgcc
     5641 acccacgcgg ccggcgtggt gccggtgccc ggcgcgctct gcgacttcgt ggtgtcgagc
     5701 tgctacaagt ggctgctggc cacccacggc gtcgggctct tcgcctacag cgcgcggcgg
     5761 gtgggcgagc ttcagccggc caccgtcggc tggcacagcg tcgggcatcg cggcgggccc
     5821 ggcgatccgc tcgcgatgtc gtggcggccg gacgcctcgc ggctcgaggc gggcaacccg
     5881 agcctgctcg gcctggccgt gctcgacagc gcgctggcac ggctcgagcg gctcgcgccc
     5941 gccgacgtgg agcgccacgc gcagggcctc gggaccgagc tgatcgaggg cctcaaggcg
     6001 cgtggctggc cggtcaccac gccggaggcg cccgaggagc gtgggggcaa cgtgtgcttc
     6061 ctggccgagg atgcgggtgg gctcgcggcg aagctggccg ccgagcgggt gctcgtctgg
     6121 ggcggagaag ggcgcatccg cgtctcgccc cacgtgcacg acgaccgcga cgacatcgca
     6181 cgcttcttcg aggcgctgga ccgcgtgggc gcccggcctc agccgagccg cagcttcatc
     6241 ccttcgtgag acgcgacgaa tcccaggccc tcgtagaagc gccgggcgtc ggggcgggcc
     6301 gctcgccgct cgccacccgt accccctcga tcagcgtccg ccagccgccc tggtaggtga
     6361 gcgagggaag ataggtgagc tgcagcacac ccacgacccg ctcgccccgg caggccacca
     6421 cgagctcgtt gttggggtct ccgtcgatgg cgcggaaggc ccgcgcgtag gagtcgggca
     6481 gcgggttctc gtagcgctcc cgtgtggcgc cgagcggatc gtccgccagc atgcgcacga
     6541 tgtcgggcac gtcctcggcg cgggctcggc ggaacgcgag cgtgacctca ggcatgcgcc
     6601 acctcgatcg ccagctcgcg cacgaagcag gcggtgcccc ccacgcgcac gtggcaccgg
     6661 ccgtcgcgcc gctccaccca cacccgaacg cgcccgtcgc ggccgatctc ctggccctgc
     6721 tcgaccagca gcggcgaggg agtgtcgtcc ccgatgaact cgagatggta cgcgccgagc
     6781 acgccggccg cggtgccggt gaccggatcc tccacggtgc ccgaccgggg cgaggagaag
     6841 tgccgcgcgt gcaggtgcgt gtcccgatgg atcgtctcga cacagaacgg gtggatggac
     6901 gcgcccggcc gctcggtcag cacggagggg aacagggcgg tgagcggctt catccgtcgc
     6961 atcgcgccga ggccatgcac cgggaccacc agggtccaga gcccggtgct gccgtagagg
     7021 atcggcaggt cggcgcgcag gtcgtccggc ccgatgccga gcacgcgcgc gagcgcggcg
     7081 cgatcacccg cgaaggattc gaactgcgcg ggcgcctggg ccatgatcac caccggatca
     7141 cccgcgccgc ccagctccac cgtgaccggc agccgcccgg cgcccgtctc cagcgtgagc
     7201 cgtcgcggaa agcccgcgcc gggcaggcgg cggtgctggt ggagggccac gacggagccg
     7261 acggtggcgt ggccgcagag atccacctca tgcccggggg tgaaatagcg cagccgcagg
     7321 tcggccctgg tggacggcag cacgaacgcg gtctcgttga agccgacggc gcgggcgatc
     7381 gcctgcatcg tcgcgtcgga gagcccgtcc gcgtcgagca cgacgccggc gggattgccc
     7441 atgccgggcc gcggcgcgaa ggcgtcgaag tgcaggacgc gcgcgggcat cgggccggcc
     7501 gcgcctcagc tccggcagcc cgcgggcttg tgcacgcggg ccgccgcggc cggaaacttc
     7561 gcgagcttgt ccgcgggccg gcgggggcgt ggcaccagct cgccgccgca gttggggcag
     7621 cggccgtgga gcacggtctc cgcgcaggcg cggcagaagg tgcactcgaa ggagcagatg
     7681 cgcgcgtcct ccgatcccgg cggcagatcc cgatcgcagc attcacagcc cggccgcagc
     7741 tccagcatgt atattctcct ctcggttggt gagcatagca tccgacccca ggaggaggag
     7801 atggccaaga gcgtggtgac gcccgagcgg ttcgccaagg gcatgacctt cgacgagtac
     7861 atgaaataca ccggcagcgc ggagaacctc gaccgcgagg ccttcggcag ctacttcccg
     7921 gacggcggcg cgaagcccgc cccgcgcaag gacaacagcg cggtgttccg cgagcgctac
     7981 gcccgggctc gcctgagcga ccaccaggtc tccgccatca ggtggctcgc ggcccagccg
     8041 ggcggccccg cgaagatcct ggtgatctcg gaggactggt cctccgactg ccggcgcgat
     8101 ctgccgtggc tcgcgcggct ggccgaggcg ggcgggctcg agctgcgtat cttcaaccgc
     8161 gacggccgga aggtgctggg cacccggcgt ccggatccgt ccgtcgcgcc cgacgggaac
     8221 cacgacatca tgctcgagtt cctcaacgcg aagagcggcg cggagtgggc ttcggtgccg
     8281 gtggtggtgg tctacaccaa ggacctccag gagctccacc gctacatcga gtaccccgcc
     8341 atctatcaca aggacccggt gcgcgcccac cagcaggccg cgcgcgacgg cgagaccgcg
     8401 gagcaggggc gggagcgcgg gatgcgggag ttcgcggcca tgcaggccgg gccgttcttc
     8461 gacctctggg cggccgcgtg cgtggacgag atcctgagcg cgctctacga gaagcggatc
     8521 ctcgcgacca cgtcagcggg ctgagcgcgg ctccatcagc tccaggcggt tgccgaacgg
     8581 gtcgttgacg tagacgcgga gatagccctc gaggggctcg tcggtagtca cgtcgtatcc
     8641 ggcgcggcgg agctcctcga tcagcgcgtc gagtccgctg accagcaggc ccgcgtgggc
     8701 cttccgcgcg gggcggaagt cggggtccac gcccaggtgg accttgaccg ggccgcgctc
     8761 gaaccaggcc ccgccgcggg ccgcgagcgc cggcggcttg gccagctcgg ggatcccgag
     8821 caatcccgcg tagaaggcgc gcgcctccgc ctcgcggccg gccggcatgg cgagctgcac
     8881 gtgatcgacg ccgacgatgg gcaagtcaac ggctcctttc cggatcagca gggcacggga
     8941 taggtgagcg ccagacgatc cgcagggtac cgcgcggtcg cgtcgaagca gccgacgatt
     9001 ctcgggagcg ggtcgtgcag atcctcggga tcgaccccgc gctgtagctc caggtgcacg
     9061 tgctcgtagc cgggccacgc gcgctggccc gaggtgccga gcgcgccgag gcgctgtccg
     9121 cgggcgacgt tctcgccgac cttcaccgcg gtttccgcca ggtggcagta gacggtccgg
     9181 tagccatggg gctcgtggac caccaccacg atcaggccgc agaggtcctg gcgctcgcgc
     9241 gccaccgtca cccggccctc cgccgcggcg agcacgtcgg tgccgacgcg tcccttgatg
     9301 tcgatgcccc ggtgaggatc caggagggga tacccctcgg ctccgcgcca agcgccgaag
     9361 cccgtgagga tgcgcggcgc cccgcccgcg gccggggcga tcatgtacac gaaccgcgcg
     9421 aagggcagga ccgcgaacag gagcgtcgcg acggcggtga cggccagccc gacgtcgggg
     9481 cggcgagctc cccgccgaag gtcgcgcccc gcccggaccg tggagagcac gagcagcgcg
     9541 ccgccgctga tcgccagggc cgggaacagg aatcgcgggg tccgctgcgc gatcgcgggc
     9601 gaggacgcgc ccaggagcac cgccgccgcg aggggaagcg cgagccggcc gagccagcgg
     9661 ctcaccgggc ggtcagtggt ggacgcggac cagccacgcg ccccggatca tcggatctcc
     9721 atggacgtga tcaggccgcc ctcgaaggcg tagacgtgct cgaccatggc gtccttgagg
     9781 acggtgccgg tcaggtcgcg caccacctgg tggacggcca ccgccacgcg cccatcggct
     9841 tccacggtga aacccgtggg ctcgacgcgc gggtcgatga gggcccactg ccgcgtccag
     9901 taggcgcgga cggcggcgtg gccgacgacg gtgcctcctt ccatgccgtt gggccagatc
     9961 acgtcggggt gcatcacgcc caggaccgtg tcgatgtcgc gcgcgttgaa ggccgcatag
    10021 gttcgggcga ggacggcccg ggcctcgtcg aaccgctcct tccgcggcgc ggcgcccgcg
    10081 gtcacgagcg cagctggtcg cgcaggaagg cgcccaccac ctcgttgtat tcctcgggga
    10141 ccagcgccgg cgcgaagtgg ccgccggtgc gcagcaccgc gagcttggcg ccggggatcc
    10201 gggccgccag ctcctccgag tagaacgccg gggtcagcgc gtcgtcggcc gcgacgacca
    10261 ccagggtcgg gcaccggatc tcgcccaggc gcgcgcgccg gtcgaagcgc acgatggcgt
    10321 cgatgcggct ggccagcacc tccaccgggg gcggggccgc gcccatgcgc ttcatctcct
    10381 cctccaggag cgcgtcgttc tcgctgatcc accggggcgg ccgcagcatg acgaccgacg
    10441 cgcgccagta gctctcgaag ccgcggtgga gcagcagctc cttgcgtgtc tcgaagcacc
    10501 ggcggaagta ggcgtcctgc ccggcccacg tggcgctcag caccaggctc cggatgcggc
    10561 ccgggtgatc ctgcgcgatc gtctggccga tggcgccgcc ggtcgagtgg ccgacgtagt
    10621 gggcggcctc gacccccagg cgatccataa ggtggatggt gtcggcggcc atctggtcca
    10681 ccgagtactg gatgcgcgag tgggtgctac ggcccgcccc gcggtgatcg tgcacgatca
    10741 cgcggaagtc ccgcgcgaag tcgcggacct gggggcccca gaaggcgccc tggccgccga
    10801 ggccgggcac cagcatcaga gcgggacccc ggccctcctc ctcatagtag atctcggcat
    10861 cgccgatgga gacgtgcggc atggcggcgg ctacttcgtg tcgttcatcc ccatgcgacg
    10921 ctcgatggcg atcacgtcgg tgggccgctc gaacggcgcg ggggcggggg cgccggcctc
    10981 gcgcgggcgg ccgatggcct cgaagaagtt ctcgagcccg ggtggggtga tgacccacag
    11041 catcaccaga tcgtcctggg cgctctcgtt gatgagctcg tgtcggacgt cgtagccgag
    11101 gaagcacgcg gtcccgggca ccagcggatg gctctggccg tccaccacca cgcggccctg
    11161 accgcggaag cagatctgta gctcgacctg atcgccgtgc gagtgctcgc gcacgcggct
    11221 gccgggggcg atggtctgga agcccatcga gtgcccggcg aacccggtgc gggcggggta
    11281 gagcttcgga tcggcgtggc cgttggcggg gacgggctgc caataggacg gaccgtcttc
    11341 cggctgcatg accaccgcgc gtccgcggac ctggtcgacc tggctggctg tctggctcat
    11401 gagggctcct cctggctcgg ggccgtgggc gccagtgtaa cccaggagcg cggtttgacg
    11461 gcccggccaa tctcggcgat agtccccgca tgaaactcac acgtgcgctc gcgatctcgg
    11521 cagtgctcct ggcgacgatc ctttccggcc cggcgccggc ccaggacgcg ccccgcccgg
    11581 gcggcgtgct gaaggccgcg atgatcggcg agccgccgac gctcgacctg cactggacca
    11641 ccgcggtcat cacgcaggag atcgccttcc acctctacga aggcctcttc acctacgacg
    11701 ccaagttcgc gccgatcccc atgctggccg agtcctacac gatgatgccc gacggccggc
    11761 gctacacctt cgtgctccgg aagggcgtgc gcttccacac cggcaaggag atgacctcgg
    11821 tcgacgtggt ggcctcgctc acccgctggg ggaaggtggc gaccaccggg aaggcggtgt
    11881 ggcgggccgt cgaggcgatc gaggccaagg gcccgcacac cgtggtgatt cacctcaagg
    11941 agccgtcggg ctcgctgctc tacgcgctcg gcagtccgaa caacggcgcg tccattcatc
    12001 cgaaggaaat catcgacgcc ccgggggacg ggcagatcaa ggagttcgtc gggaccgggc
    12061 cctaccgctt cgtcgagcat cggcccgacc ggcacatcaa ggtcgcccgc ttcaaggact
    12121 acgcggcccg ccgcgaggcg ccggacggct acggcggtcg gcgcgccgcg tacgtcgacg
    12181 agatcctgtt catcccggtt cccgacgtgg cggtgcggct ggccggcgtg gagaccgggg
    12241 agtaccactt cgcgaacacc atcaacccgg acaagtacga ggggatcaag gggcgcgccg
    12301 acatcaccgc cgcggcggtg aagccctacg gctgggtcac cgcggtgccg aaccacaagc
    12361 agggcgtgat ggccaacaag aaggtccgcc aggcgctcca ggccgtgctc gacatggagc
    12421 cgatcatggc ggccggcatc ggcaacaagg acttctatcg gctctccggc acgctcttct
    12481 atccggagca agcggcgttc tactcgacca ccggcgtcac cggatacaac ctcaagaaca
    12541 aggaccgggc ccgcgcgctc ctcaaagagg cgggctacgc gggccagccc gtgcgctggg
    12601 tcaccacgaa ggaatacgag tggatgtaca acaccgcggt ggtggccaag cagcagatgg
    12661 aggaagctgg cttcgtggtg gacttgcagg tggtggactg ggcgacgctg gtccagcgcc
    12721 ggaacaagcc ggagttgttc gatcttttct ccaccggctt cactttcaac gcggacccgg
    12781 cgctcgcgtc gtccttgcag tgcaactggc ccgggtggtg gtgcgccgag gagaaggaca
    12841 agctgatggc cgatctgacg cgcgagaccg atcccaggaa gcggcgggcg atcatcgagc
    12901 gcctgcaggc gatcttctac gaggacgtgg ggcgcatcaa gctcggcgac tactatcagc
    12961 tgcaggcgat gcgcaaggat ctgcgcgggt tcaagcccgg cccctacctc tacttctgga
    13021 gcgcctggct ggcgaagtaa cgggcccctc accacccagc ccctcacctt gccctctccc
    13081 cagaggggag aaggtgggaa gcgggactcc ctctcccctc tggggagagg gctggggtaa
    13141 ggggtggtct aggaaggccg cagcacccgc cccggcagcg cgccggtgtg ctccccatcc
    13201 gcaatgacga cgcgcccgtt gaccagcacg tggccgatgc cggtggcgta ccggtgcggg
    13261 tcctcgtagg tcgcgcggtc ggccaccgtc gcggcatcga acaccaccac gtcggccttg
    13321 gcccccggcg ccagcacccc gcgatcgctc aagcctaggc gccgcgcggg catccccgtc
    13381 atcttccaca ccgcgtccgg gagcgacagc acgcgctcgt cgcgcgcgta gcggccgagc
    13441 acgcgcggga acgtgccgta gctgcgcggg tgcggcttcc cggtggcgag cgggccgtcg
    13501 gcggccagtg acgacccatc ggagccgatc atgacgtgcg ggtgcgtgag cgtccggcgc
    13561 aggtcggcct cgtcgagctg gaagaggatc atgtagccct tgccctgctc ggccacgatc
    13621 agctcgatgg ccgcgtcgag cgggtcttct ccccgcgcgg ccgcgatctc cgcgatgcgc
    13681 cggccctgtg cgtccggccg cgacggcgag taggccacca tgatgtcggc ccagtcgagc
    13741 ccgcgcagca gggtctcgcc gccgccggtc aggtccttgc ggatgcgctc gcgcgtggct
    13801 cggtcggcga ggcgcttcag catggcgtcg ataccgccct cgagcaccca gtcgggcaac
    13861 agcgtgcgga gcgtggtgct ggaggcagtg tagggataga cgtcgcccat gacgtcctgc
    13921 ccccgggcgc gcgccgcgtc gatcagcgcg agcgcgtccg cgacccggcc ccagttcggc
    13981 cgcccggccg ccttgacgtg gctcacctga gtgggcagcc cgccctcgcg cccgacccgg
    14041 atggcctcgg ccaccgcgtc gagaagggtc gcgccctcgc cgcggatgtg actcgcgtag
    14101 aagccgcggc ggccgcccgc gcgccgcgcg atgccgatga tctcctcggt ggccgcatag
    14161 gagccgggcg cgtagatgag gccggtggcg aggccgtagg cgccgtcggc catggcctcg
    14221 tccatcatgc gctccatcgc ggcctgctcg gccgcagtgg gcggccggcg cgcgaagccc
    14281 atggcggcca ggcgcagcgt cccgtggccg atgagctggg ccacgttcag cgcgatgccc
    14341 tcgtcctcgt accgcgcgag gaactcgcgg accgacgtcc acgagaagtc catccccgcg
    14401 ggcaggtaga gggcgaagcc cttcatctcg tcacggaagg cgtcggagac cggggcgggt
    14461 gagaagccgc agttgccgac cacctcggtg gtcacgccct ggcggatctt cgactcggcg
    14521 cggcggttgt cccagaggcg ccagtcggaa tgcgagtgca tgtcgatgaa gccgggggtg
    14581 acggtgcggc ccgtggcgtc gatcgtgcga cccgcgggct cggccgagag atcgcccacc
    14641 gtggtgacgc ggtcgccggt gaggcccaca tcggcacggc gcgcgggcgc accggtgccg
    14701 tcgatgacct gcccgcccgt gatcttgatg tcgagcacgg gtgctagtag cggcggtcgt
    14761 ccaggtcgag cagcgggaag gcgctcgtga cgtgcggcag ggtgaccggt gtggccgggt
    14821 ggaggtacga gctgtcgagc tgggccaggc tgtgcacgcc gaggaggccc atcgcgatgc
    14881 ggatctcctc ctccagcagc tcgagcgcgc gcaccagccc cggctcgccc gcggcggcca
    14941 gcccgaggca ggagaggcgc ccgatcccca ccatctgcgc gccgagcgcg atggccttga
    15001 ccacgtcggt gccgcgcatg aaaccgccgt ccaccatgac cacgctgcgc cccgcgacgg
    15061 ccttcaccac ttccggcagc acctcgatgg acccgcggcc gtgatcgagc tggcggccgc
    15121 cgtggttgga gacgtagacc gcgtccaccc cgtgatcgca cgcgatggcg gcgtcctcgc
    15181 cggtggcgat gcccttcagg atcagcggga ggtcgtggcg gtccttgaag cgcttgacct
    15241 ggtcccacga gagcgcggcc tggaa
//
LOCUS       DASUFA010000010         6495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_804593, whole genome shotgun sequence.
ACCESSION   DASUFA010000010 DASUFA010000000
VERSION     DASUFA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804593"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFX87_00390"
     CDS             <1..683
                     /locus_tag="VFX87_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4993385.1"
                     /translation="VLTGAGRGFCAGADMQMLSGIVGAGRLETGDAASGREPAATGGD
                     AAIRPDFRGPYAYFPMVPKPIIAALNGATAGLGLVVSLYCDLRIAADSAVFTTAFSRR
                     GLIAEHGVSWMLPRLVGLQHALDLLLSARKITAAEALAMGLVAQVRPAATLQGDVRAY
                     ARELADLVSPRSLRVMKRQIWDAQFQSLAEATAVGDEEMRKSFGAEDFKEGVAHFVEK
                     RAPRFTGR"
     gene            687..914
                     /locus_tag="VFX87_00395"
     CDS             687..914
                     /locus_tag="VFX87_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993386.1"
                     /translation="MARLDILPPDAIADPELRGMAEASGDEMYGVYGHCPELFAAFLR
                     FYRPAKYGGRLPFALKELVRLKIAALNECAR"
     gene            1071..1229
                     /locus_tag="VFX87_00400"
     CDS             1071..1229
                     /locus_tag="VFX87_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993387.1"
                     /translation="MTEMRRHFTDAELVELGLITGAFIMLGRLHRTFGVAPMGPRSHA
                     VLERGYSS"
     gene            1226..2272
                     /locus_tag="VFX87_00405"
     CDS             1226..2272
                     /locus_tag="VFX87_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4993388.1"
                     /translation="MTIIWKVLLAALVVALALGPAAAPVPAFDGEKALHEAAKKEKEF
                     TWYTAHYNSETAAALCQGFEKKYPGVKCNYIRTTAQVAYQRLAQDQKAGIAQASVISS
                     TDQSHYTKMKQDGWLQPYKPKNLPELVDAFRSFNDPDDLFTATAAGLVLITYNTSVVS
                     PAEAPKKWTDLTDPKWKNKVSIGHPGFSGYVGTWVVQMRKLYGWDYFKKLELNKPRIG
                     RSINDTVTMLNAKESIVAAGPSATTLESRAKGNPVAVVYPEDGALLMVSASGILKNAP
                     APNAAKLFMEYLLSREGNEVMVQQYQDPVNKHVKPMAGGKSIAEVKTIRPTAVEIEKG
                     IPEVKELFRETFGI"
     gene            2294..3970
                     /locus_tag="VFX87_00410"
     CDS             2294..3970
                     /locus_tag="VFX87_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4993389.1"
                     /translation="MKDRSLVVWVLLIALLLLLVINPLARLLWASFEHPETGAFTLGN
                     YAAAYGRWRNIEALINSLEVGLAAAALCIVFGVPMAWAVSRTDMPAKGLVWAAVLGTF
                     IMPNYLGAVAWILMAGPNAGWLNRIYTGLTGAAAGPFNIYSKTGLVLVIACYSFPYMF
                     VFTRSALDLISSEMEDAASTLGASALRTTLRVTLPLALPAIMGAFIVEFLEAIALFGA
                     PALIALPARFQVMTTMLWQMFEFPPRLQVAAAYSMPLLLITVFLFWLQYRLIARKGFV
                     SLTGKGGERRMVRLGAWRWALLGYCLFVTSLSFFVPMVVVLQAAFAKAWGRGFGWDNL
                     TLRNVRFVLFDQTATRAATVNTFVYAAAASVIALGLALAIAYIVARRLVPMGRALTYV
                     AMAPFVIPGIVLAIGFYAAYTTKPLVLYGTAWILILAFATRFLPIAYTNSDASLRSIN
                     PELEDAARILGGSRLLALRRVVVPLLKRGLAGAFILVFIPATRELSSAIFLYTTGTQV
                     LSVLLFDKSDEGNFEILSSMGLILVVGTVALVLAGFRLVGRDFLLRRTAS"
     gene            3967..5043
                     /locus_tag="VFX87_00415"
     CDS             3967..5043
                     /locus_tag="VFX87_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4993390.1"
                     /translation="MSRLVLTEVSRRFGSVTAVDRFTLSLAQGEFVSLLGPSGCGKTT
                     TLRMIAGFLEPSAGTIEMDGRTISSPTATLPPEKRGMSMIFQSYAIWPNMTVAENVAF
                     GLEVRRLPKAEIRARVDKILDVVQMGHLGGRYPQELSGGQQQRVALARAIVVQPAVLL
                     LDEPLSNLDANLREEMRYEIRRLHDEFHITTVYVTHDQAEAMVTSDRIAVMHQGRIEQ
                     VDAPRALYARPRTRFVAGFIGRANFLDGEARGEAIVFDHFEIPRGAFAEPQTLRGRVS
                     FSLRPQSIVLHRQPPAASERGAVVPGAVTQRAYLGEHWDYTVRPAGSALALRVTARPH
                     EVFEVEEAVWLELDPRQMAPLLPE"
     gene            4995..5591
                     /locus_tag="VFX87_00420"
     CDS             4995..5591
                     /locus_tag="VFX87_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4993391.1"
                     /translation="MARAGPASDGAAPARVRLILLNKPYGVLCQFTDTAGRPTLARYV
                     SVSGVYPAGRLDHDSEGLVLLTDDGALQARLAEPRHGVEKTYWAQVEGVPAEAALEQL
                     RAGVVLKDGRTRPARVRLIDEPPRLWPRDPPIRYRAAIPTSWLEIGIREGRNRQVRRM
                     TAAVGHPTLRLIRVAIGPYALGTLPPGQWRAEKPVRFR"
     gene            complement(5649..6425)
                     /locus_tag="VFX87_00425"
     CDS             complement(5649..6425)
                     /locus_tag="VFX87_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993392.1"
                     /translation="MAYQAFDLTGKTAIVTGGNSGIGLGMAEAMAHAGAAVCIWGTNE
                     EKNKTALARLHAIGGKALALRCDVSDEAAVEHAFGETVSVLGGVDACFVNAGVSGRGG
                     ATSFAEMTTQEWHRVLAVNLDGAFFTLRAAARHMTARGAGGSLAITASLAALEGSPLS
                     AHYAASKGAMLSVMRALSVGLARHKIRVNAIVPGWIDTPMTEKAVHGDVFKTRVLPRV
                     PVRRWGTGDDFGGIAVYLASDASSYHTGDTFVIDGGYAIF"
BASE COUNT          923 a         2370 c         2195 g         1007 t
ORIGIN      
        1 tcgtgctgac cggcgccggc cgcggcttct gcgcgggcgc ggacatgcag atgctctccg
       61 gcatcgtggg cgcgggccgg ctggagacgg gcgacgccgc gtcagggcgc gagcccgcgg
      121 ccaccggcgg cgacgcggcc atccgtccgg acttccgcgg cccctacgcg tacttcccga
      181 tggtgcccaa gccgatcatc gcggcgctca acggggccac cgccgggctc ggcctggtcg
      241 tctcgctcta ctgcgatctg cgcatcgccg ccgacagcgc ggtgttcacc accgcgttct
      301 cgcggcgcgg gctcatcgcc gagcacgggg tgagctggat gctgccgcgc ctggtgggcc
      361 tgcagcacgc cctcgacctg ctgctctccg cgcgcaagat caccgccgcg gaggcgctcg
      421 cgatgggcct ggtcgcccag gtgaggccgg ccgcgacgct ccagggggac gtgcgcgcct
      481 acgcgcgcga gctggccgat ctggtatcgc cgcgctcgct ccgggtcatg aagcgccaga
      541 tctgggacgc gcagttccag agcctggcgg aggcgacggc ggtgggagac gaagagatgc
      601 ggaagagctt cggcgccgag gacttcaagg agggcgtggc ccatttcgtg gagaagcgtg
      661 cgccccgctt caccgggcgg taggcgatgg cccgactcga catcctccca cccgacgcga
      721 tcgccgatcc ggagctccgc ggcatggccg aggcctccgg cgacgagatg tacggcgtct
      781 acggccactg ccccgagctc ttcgcggcct tcctgcgctt ctaccggccc gcgaagtacg
      841 gcgggcggct gcctttcgcg ctgaaggagc tggtgcggct caagatcgcc gcgctcaacg
      901 aatgcgcgcg ctgagcggcg gctcgcgagc cctcggagag ggccccggga ctgtacctcg
      961 agctgctgcc gaagctcgcc ggggagacgg agccgagcga gcgcgagcgg gccgcgctgg
     1021 cgttcgcgga gaagctcgcg acgaatcaca ccgcgatcga cgatgcgttc atgaccgaga
     1081 tgcgcaggca cttcaccgac gccgagctgg tggagctggg gctgatcacc ggcgccttca
     1141 tcatgctcgg gcggcttcac cggaccttcg gcgtggcgcc gatgggtccc cgcagccacg
     1201 cggttctcga acgggggtac agctcatgac gatcatctgg aaagttctcc tggcggctct
     1261 cgtcgtcgcg ctcgcgctcg gcccggcggc agcacccgta ccggccttcg acggcgagaa
     1321 ggcgctccac gaggcggcga agaaggagaa ggagttcacc tggtacaccg cccactacaa
     1381 ctcggagacc gcggccgcgc tctgccaggg cttcgagaag aagtaccccg gcgtgaagtg
     1441 caactacatc cgcaccaccg cccaggtcgc gtaccagcgg ctagcccagg accagaaggc
     1501 gggcatcgcc caggcctcgg tgatcagctc caccgatcag agccactaca ccaagatgaa
     1561 gcaggacggg tggctccagc cctacaagcc caagaacctg cccgagctgg tggacgcctt
     1621 ccgctcgttc aacgatccgg acgacctctt caccgccacc gcggccggcc tggtgctgat
     1681 cacctacaac accagtgtgg tctcccccgc ggaggcgccc aagaagtgga ccgacctcac
     1741 cgatcccaag tggaagaaca aggtctcgat cggccacccc ggcttcagcg gctacgtggg
     1801 cacctgggtc gtgcagatgc gcaagctcta cggctgggac tacttcaaga agctcgagct
     1861 gaacaagccg cgcatcggcc gctcgatcaa cgacaccgtg accatgctga atgccaagga
     1921 gtccatcgtg gccgcggggc cgtcggccac cacgctcgag agccgggcca agggcaatcc
     1981 cgtcgccgtg gtctatccgg aggacggggc cctgctgatg gtctccgcct ccggcatcct
     2041 caagaacgcc cccgccccca acgcggccaa gctcttcatg gagtacctgc tgagccgtga
     2101 gggcaacgag gtgatggtgc agcagtacca ggacccggtg aacaagcacg tgaagccgat
     2161 ggcggggggc aagtcgatcg cggaggtcaa gaccatccgg ccgaccgcgg tcgagatcga
     2221 gaagggcatt cccgaggtca aggagctgtt ccgcgagacg ttcgggatct gaggggcggg
     2281 ctccggtcgc gccgtgaagg acaggtccct cgtcgtctgg gtgctgctga tcgcgctgct
     2341 gctgctgctc gtgatcaacc cgctcgcccg cctcctgtgg gcgagcttcg agcatccgga
     2401 gacgggcgcc ttcacgctcg ggaactacgc ggccgcctac ggccgctggc gcaatatcga
     2461 agcgctgatc aactccctgg aggtcgggct cgcggcggcc gcgctctgta tcgtgttcgg
     2521 ggtgccgatg gcctgggcgg tgtcccgcac cgacatgcca gccaagggcc tggtgtgggc
     2581 cgccgtcctc ggtaccttca tcatgcccaa ctacctgggc gcggtggcct ggatcctgat
     2641 ggccggcccc aacgcgggct ggctcaaccg catctacacc gggttgaccg gggccgccgc
     2701 cggccccttc aacatctaca gcaagaccgg acttgtgctc gtcatcgcct gctactcctt
     2761 cccctacatg ttcgtgttca cccgctccgc cctcgacctg atctcctcgg agatggagga
     2821 cgcggcgagc acgctggggg cctcggcgct ccgcaccacg ctccgggtga ccctgccgct
     2881 cgccctgccc gcgatcatgg gcgccttcat cgtggagttc ctggaggcga tcgcgctctt
     2941 cggcgccccc gccctcatcg cgctgcccgc ccgcttccag gtcatgacca ccatgctctg
     3001 gcagatgttc gagttcccac cgcgcctcca ggtggcggcg gcctactcga tgccgctgct
     3061 cctgatcacc gtgttcctct tctggctgca gtaccgcctc atcgcccgca agggcttcgt
     3121 gtctctcacc ggcaagggcg gcgagcggcg catggtgcgc ctcggcgcct ggcgctgggc
     3181 cctgctcggc tattgcctgt tcgtgacctc gctctcgttc ttcgtgccga tggtggtggt
     3241 gctccaggcc gccttcgcga aggcttgggg ccgcggcttc gggtgggaca acctcaccct
     3301 gcgcaacgtg cgcttcgtcc tcttcgacca gaccgccacc cgcgcggcca ccgtgaacac
     3361 cttcgtctac gcggcggccg cctcggtcat cgcgctcggc ctggcgctgg ccatcgccta
     3421 catcgtggct cggcggctcg tgccgatggg ccgggcgctg acctacgtgg cgatggcccc
     3481 gttcgtgatc ccgggcatcg tgctcgccat cggcttctac gccgcctaca ccacgaagcc
     3541 cctggtgctc tacggcaccg cgtggatcct catcctcgcc ttcgccaccc gcttcctgcc
     3601 catcgcgtac accaacagcg acgcgtcact gcgcagcatc aaccccgagc tcgaggacgc
     3661 ggcccggatc ctgggcggga gccggctcct ggccctgcgc cgcgtggtgg tcccgctcct
     3721 caagcgcggg ctcgcgggcg ccttcatcct cgtcttcatc ccggccaccc gcgagctgtc
     3781 ctccgccatc ttcctctaca ccaccggcac gcaggtgctc tcggtcctgc tcttcgacaa
     3841 gagcgacgag ggcaacttcg agatcctctc ctcgatgggc ctgatcctgg tggtgggcac
     3901 ggtggcgctc gtgctggccg gcttccgcct ggtcggtcgc gacttcctcc tgcggaggac
     3961 cgcctcgtga gccgcctggt cctcaccgag gtgagccgcc gcttcggcag cgtgaccgcg
     4021 gtggaccgct tcactctctc tctcgcccag ggtgaattcg tctcgctgct cgggccctcc
     4081 ggctgcggca agaccaccac gctccggatg atcgccgggt tcctcgagcc gagcgcgggc
     4141 accatcgaga tggacggccg cacgatctcc tcgcccaccg cgaccctgcc gcccgagaag
     4201 cgcggcatgt ccatgatctt ccagagctac gcgatctggc cgaacatgac ggtggccgag
     4261 aacgtggcct tcggcctgga agtgcggcgg ctcccgaagg cggagatccg cgcccgggtg
     4321 gacaagatcc tggacgtggt gcagatgggg catctcggcg gtcggtatcc tcaagaattg
     4381 tcgggcggcc agcagcagcg cgtggcgctg gcgcgcgcga tcgtggtgca gcccgcggtg
     4441 ctcctgctcg acgagccgct cagcaacctc gacgcgaacc tgcgcgagga gatgcgctac
     4501 gagatccggc gcctccacga cgaattccac atcaccaccg tctacgtcac ccacgaccag
     4561 gccgaggcca tggtcacctc cgaccggatc gcggtgatgc accaagggcg gatcgagcag
     4621 gtggacgccc cccgcgcgct ctacgcgcgc ccgcgcaccc ggttcgtggc gggcttcatc
     4681 ggccgcgcca actttctcga cggcgaggcg cggggcgagg cgatcgtctt cgaccacttc
     4741 gagatccccc ggggcgcgtt cgcagaaccc cagacgctcc ggggccgcgt gagcttctcg
     4801 ctgcggccgc agtccatcgt gctgcaccgc cagccgcccg ccgcatccga gcgcggcgcg
     4861 gtcgtgccgg gcgcggtgac ccagcgcgcg tacctcggcg agcactggga ctacacggtg
     4921 cggccggcgg ggagcgcgct ggccctccgg gtcaccgccc gcccgcacga ggtcttcgag
     4981 gtggaggagg ccgtctggct cgagctggac ccgcgtcaga tggcgccgct cctgcccgag
     5041 tgaggctgat cctcctcaac aagccctacg gcgtgctgtg ccagttcacc gacacggcgg
     5101 gccggcccac gctcgcccgc tacgtctcgg tctcgggcgt ctacccggcc ggccgcctcg
     5161 accacgacag cgaggggctc gtgctgctca ccgacgacgg cgcgctccag gcgcgtctgg
     5221 ccgagccacg gcacggcgtg gagaagacct actgggcgca ggtggagggc gtgcccgcgg
     5281 aggccgcgct cgagcagctc cgcgccggtg tggtcctcaa ggacgggcgc acgcggcccg
     5341 cgcgcgtgcg gctcatcgac gagccgcccc ggctctggcc gcgcgatccg ccgatccggt
     5401 accgcgcggc gatccccacg tcgtggctgg agatcgggat ccgcgagggg cgcaaccggc
     5461 aggtgcgccg catgaccgcg gcggtcgggc atccgacgct gcggctgatc cgggtcgcga
     5521 tcgggcccta cgcgctcggc acgctgccgc cgggccagtg gcgcgccgag aagccggtgc
     5581 ggttccggta acaccgcccc tcaccctacc gcccctcacc cgactctctc ccctgagggg
     5641 agagggctct agaagatggc gtagccgccg tcgatgacga aggtgtcgcc ggtgtggtag
     5701 ctgctggcgt cgctggccag gtacaccgcg atgccgccga agtcgtcgcc ggtgccccag
     5761 cggcgcacgg ggacccgtgg gagcacgcgc gtcttgaaga cgtcgccgtg gacggccttc
     5821 tcggtcatcg gcgtgtcgat ccatccgggc acgatggcgt tgacgcggat cttgtgccgc
     5881 gcgagcccca ccgagagcgc gcgcatgacc gagagcatgg cgcccttgct ggccgcgtag
     5941 tgggcgctca gcggcgagcc ctccagcgcg gccaggctcg cggtgatcgc cagcgagccg
     6001 ccggccccgc gcgcggtcat gtgccgggcc gccgcgcgca gggtgaagaa cgcgccgtcg
     6061 agattcaccg ccagcacccg gtgccactcc tgggtggtca tctccgcgaa gctcgtggcg
     6121 ccaccgcggc cggacacgcc cgcgttgacg aagcaggcat ccaccccgcc gaggacggac
     6181 acggtctccc cgaaggcgtg ctcgaccgcc gcctcgtcgc tcacgtcgca gcggagcgcc
     6241 agggccttcc ccccgatcgc gtggaggcgc gcgagcgcgg tcttgttctt ctcctcgttg
     6301 gtgccccaga tgcagaccgc ggcgccggcg tgcgccatcg cctccgccat gccgaggccg
     6361 atgccgctgt tgccgccggt gacgatcgcg gtcttgccgg tgagatcgaa ggcctggtac
     6421 gccatggtga cgtctcctcc tacggccgca tgcgatgggt ggacggaccc acttcgcgca
     6481 gcggcgcggc cagct
//
LOCUS       DASUFA010000011         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_919545, whole genome shotgun sequence.
ACCESSION   DASUFA010000011 DASUFA010000000
VERSION     DASUFA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919545"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFX87_00430"
     CDS             <1..430
                     /locus_tag="VFX87_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4993393.1"
                     /translation="FLTASGRTLAVDCSRRTVAYRGRRYEIVIYRDAAERLAGEEARR
                     EAAALRSVNLLAQAAAHEISNPLAIIMGYSQLLEDRLVAETEEGGWARTCRRAGGRIR
                     DAVGRLNRIVRIESTESTGSLPPILDTERSAGPHDDDARR"
     gene            417..1664
                     /locus_tag="VFX87_00435"
     CDS             417..1664
                     /locus_tag="VFX87_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993394.1"
                     /translation="MPAANPGARPGPPRLFHGWIVVAGAFLILFMAYGTQYAFGVFFA
                     ALIDEFGWSRASLSGVFSLYAFTYAAFALVSGRLTDRWGPRAVIAIGGVLLGLGLIAM
                     SHVSLLWQPYVCYGLVAALGMSTAYVPCNATVAKWFTRRRGLAIGLASAGGSLGTFAL
                     PPVAHVVVSRLGWRWAYVVFGVAVLLALNALAVLMRRDPETIGLGPDGDTRPRDATAR
                     PEGWTAARAIRTRAFWLLFAVFSATWIPVFVPLVHLVPMARGLGVSPLLAATLVSALG
                     AAALVGRLLMGGASDRIGRRAALAVALVLQAAAFVGFAQARELPALYAASLVFGFSYG
                     AGSTLFPAAVADFFGREQAASLAGLLFALAGSMAAWGPLAAGFIYDRAGDYQLAWWLS
                     AGFNVLALVLLAFTRPPASRSLA"
     gene            1711..>2496
                     /locus_tag="VFX87_00440"
     CDS             1711..>2496
                     /locus_tag="VFX87_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993395.1"
                     /translation="MASLKDKYCIVGVGETEYSRQSARSTRAMAVEAVRNAMRDAGLG
                     KDGVDGMMSYQSGDSVFANWVAPDLGIRLNFYMDVFGGGSSTEALVGLAMGAIEAGMC
                     HTVAIFRSMNGYSDFRIGGTGARAAQPVRGLDLAQVPFGMRSAGQNFAPTFMRHMYEY
                     GTTSEQVAHVRVAHSKHASQNPKAFLKDRVTVADVVGSRWICKPLHLLDCCLETDNAT
                     ALIVTSAERARDLRRKPVYIMGVAGRVNKPRTDFHWAHGPITRV"
BASE COUNT          294 a          935 c          876 g          391 t
ORIGIN      
        1 gttcctgacc gcctcggggc ggacgctggc ggtggactgc tcccgccgca ccgtcgcgta
       61 ccggggccgg cgctacgaga tcgtgatcta ccgcgacgcg gccgagcggc tcgcgggcga
      121 ggaggcgcgg cgggaggccg cggcgctccg atcggtgaat ctacttgccc aggcggccgc
      181 ccacgagatc agcaatccgc tcgcgatcat catgggctac agccagctgc tggaggaccg
      241 gctggtcgcg gagaccgagg aaggcgggtg ggcccgcacc tgccgccgcg ccgggggtcg
      301 cattcgcgac gcggtcggac ggctcaaccg catcgtccgc atcgagagca ccgagtccac
      361 cggctcgctg cctccgatcc tggacacgga gcgatcggcg gggccgcacg acgacgatgc
      421 ccgccgctaa ccccggcgct cggccggggc cgccccgtct cttccacggc tggatcgtgg
      481 tggcgggcgc cttcctgatc ctcttcatgg cctacggcac ccagtacgcc ttcggcgtct
      541 tcttcgccgc cctcatcgac gaattcgggt ggagccgggc cagtctctcc ggggtcttct
      601 cgctctacgc gttcacctac gcggccttcg ccctcgtctc gggccggctg accgatcgct
      661 gggggccgcg cgcggtcatc gcgatcggcg gcgtcctgct cgggctcggg ctgatcgcca
      721 tgagccacgt cagcctgctg tggcagccct acgtgtgcta cggcctcgtg gccgcactgg
      781 ggatgtccac cgcctacgtg ccgtgcaacg ccacggtggc gaagtggttc acgcgacgtc
      841 gcggccttgc catcgggctg gccagcgcgg gaggcagcct cggtaccttc gcgctgccgc
      901 cggtcgccca tgtcgtggtg agccggctcg ggtggcgctg ggcctacgtg gtcttcggcg
      961 tcgcggtcct gctcgccctg aacgcgctcg ccgtcctcat gcggcgcgac ccggagacga
     1021 tcggcctcgg ccccgacggg gacacgcgcc cgcgggacgc gaccgcgcgc ccggagggct
     1081 ggacggcggc ccgggcgatc cggacccgcg cgttctggct gctcttcgcg gtattcagcg
     1141 cgacgtggat tccggtgttc gtcccgctcg tgcacctggt gccgatggcc cgagggctcg
     1201 gcgtctcgcc gctgctggcc gcgaccctcg tcagcgcgct gggcgccgcc gcgctggtcg
     1261 gccgcctgct catggggggc gcgtcggacc ggatcgggcg gcgggccgcc ctcgcggtgg
     1321 ccctcgtgct gcaggccgcc gccttcgtcg gcttcgcgca ggcgcgcgag ctgcccgcgc
     1381 tctacgcggc atcgctcgtc ttcggcttct cctacggcgc cggctccacc ctcttccccg
     1441 ccgcggtcgc cgacttcttc ggccgcgagc aggccgcgag cctcgccgga ctgctcttcg
     1501 cgctggccgg gtcgatggcc gcgtggggcc cgctcgcggc cggcttcatc tacgaccgcg
     1561 cgggcgacta ccagctcgcg tggtggctca gcgccggctt caacgtgctc gccctggtcc
     1621 tcctcgcctt cacccgcccg cccgcttcgc gatccctcgc ttgacggccc ccgccacgct
     1681 cgcgtaaagt cccggcgcgg aggattcgcc atggcatcgc tcaaggacaa gtattgcatc
     1741 gtcggggtcg gggagaccga gtacagtcgg cagtccgcgc gcagcacccg cgcgatggcg
     1801 gtcgaggcgg tgcgcaacgc catgcgcgac gccggcctcg gcaaggacgg cgtggacggc
     1861 atgatgtcct accagagcgg cgactccgtc ttcgccaact gggtggcgcc cgatctcggc
     1921 atccggctca acttctacat ggacgtcttc ggcggcggct cgtccaccga ggcgctcgtc
     1981 ggcctcgcca tgggcgccat cgaggcgggc atgtgccaca cggtggcgat cttccgctcg
     2041 atgaacggct actccgattt ccgcatcggg gggaccggcg cccgcgcggc ccagccggtg
     2101 cgcggcctcg acctggccca ggtgcccttc ggcatgcgca gcgccggcca gaacttcgcg
     2161 ccgaccttca tgcgccacat gtacgagtac gggacgacgt cggagcaggt ggcccacgtg
     2221 cgggtggccc acagcaagca cgcctcgcag aacccgaagg cgttcctcaa ggaccgggtc
     2281 acggtggcgg acgtggtcgg ctcccggtgg atctgcaagc cgctccacct gctggactgc
     2341 tgcctcgaga ccgacaacgc caccgcgctg atcgtcacga gcgcggagcg ggcgcgcgac
     2401 ctgcggcgca agcccgtcta catcatgggg gtggccggcc gggtcaacaa gccgcgcacc
     2461 gactttcact gggcccacgg cccgatcacc cgggtc
//
LOCUS       DASUFA010000012        21705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_690488, whole genome shotgun sequence.
ACCESSION   DASUFA010000012 DASUFA010000000
VERSION     DASUFA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 21705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21705
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690488"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1507
                     /locus_tag="VFX87_00445"
     CDS             143..1507
                     /locus_tag="VFX87_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4993396.1"
                     /translation="MLAIPPGLMRWIFGIVLAASLLAMGCNGPDSRARGSIKAGDEAA
                     QQRALREALNHYRTAAVAEPASLEAQTRRGAMAELLGEFDEALDAYSRASRLQPSGLA
                     YYRAGAMADRMGNTVLAAEYLNASLQAPPTRGERWAQTGQHAIERVTLSLNGSRWGRM
                     LPDWVFRSLHATRVALANAVLDREVVAAALFTSLLEAGENERALEVARGRGWVRAGAD
                     YCAAGRGVVGAETRALVGMLAAPERADCLLPLGRALTDAGLVRLSRLVLQDRIRRAPD
                     LRARREAEAFLRYRLPAHDVPKTAESLNIAAYNLQHRFGDQGAAAAAYQKAIAADPKF
                     SWPYANLGRLYMELDQYDLAVDWLGNAIRINPNHFRAHANLGVALQTLRRYDEAVGAY
                     RAALALNPDDAATHANLGRSLLSLGRDAEGLRSLQTAVRLDPRLEEERELLTRRLAGG
                     FRLD"
     gene            1531..2766
                     /locus_tag="VFX87_00450"
     CDS             1531..2766
                     /locus_tag="VFX87_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993397.1"
                     /translation="MRPDPTASPAPSYLGLGVTVVTLALAYGVWYAYSVFLVALLREF
                     GWSRSVLAGAFSIFTLVSGGAGPVLGALADRFGPRRLILIGGVLLAGSLWADSLVTRA
                     WHLYLTFGLLTAVGVATAGWTPAVVMVQRQWKARLGLALGIAGSGVGLGIFLVVPLCQ
                     ALIDGFGWRWAFRALAILCALWILPATFLAIRDTPPSSPREPAARGETGRPAGGEHSL
                     GLALANPSFRLIGLAVFLGSICSQTLHVHQAAFLVDHGLPAMTAASVISAVGASSIVG
                     KTGGGWISDHLSRELVYALGMIGMIFGVGVLWLVALAPSAWLALGYAVLFGVGYSVTA
                     FIVPAMMSDRFRGPHFGSIFGATQVASALGSALGAWLAGRIFDATGSYAIAFSLAAAA
                     AGVAALSVWAGRLRGPARP"
     gene            complement(2763..3299)
                     /locus_tag="VFX87_00455"
     CDS             complement(2763..3299)
                     /locus_tag="VFX87_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993398.1"
                     /translation="MASPERNISAVDQRLVSLEDEATQLRAEVAALQEEIRWLTGQDE
                     DEHGWLARGWVRASLLLTAVGLVALVSLPYLLHLDASGPNADPPPGAAASARPAPAAP
                     RATVEPAPAPVKAAARDAAPAPAPARVRAPEVPEPARVLSAEDHAATAPRPRPVRERR
                     ALQASDVVVVAPARGESP"
     gene            3415..4203
                     /locus_tag="VFX87_00460"
     CDS             3415..4203
                     /locus_tag="VFX87_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4993399.1"
                     /translation="MTIPDILALDFDGVCCDGLREYFETSRRTWARVWPADEPPADSA
                     FVAFCALRPVILSGWEMPVLLRAIARGVPRAEILGGWERVRAELVATGSPSGDALIAR
                     LAHALDEVRREWIAADEPGWLALHAPYGSVDELRRLVAGPGRSVLVTTKEGAFARRIL
                     DSWGVRLADVQGKETGAHKCLNLRALIAAWTAERGSRPRLAFVDDRLETLRHVTTHPD
                     LDDVALFLAAWGYNTEAARAAARADGRIRLLTLEQFRRGAAAWS"
     gene            4313..5554
                     /locus_tag="VFX87_00465"
     CDS             4313..5554
                     /locus_tag="VFX87_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX4993400.1"
                     /translation="MKIARVEALHCDGGWRPWTFVRVETDDGLVGWGECSDNRSPYGI
                     AGSVRDLAPLLIGQDPRPVERLYWDMLRATRQNLGGVTHKAMAGIELALWDIKAKALG
                     VPVYELFGGPLRDRMRLYWSHCGTTRARLGHVLGTPPLRTYDDIAALGKEVVARGFTA
                     LKTNMVIPGDPATVYFPGFASGINSTDGAPTVEILGAIERLIGTFRDAVGPKVGLCLD
                     LNYNFRTEGVLRVAKLLEQFDMQWVEYDNWDPQALLQIKQSTSTRLASLESLVTTRQY
                     RPFLELHAVDVAIIDVPWNGFSQAVQIGRMAEAYEINIAPHNYYSHLADLHSLHLCAV
                     LPNVRIMEIDIDDVSWKADLVTKPPVVKDGHVFLSDAPGWGADINEEVLRAHPWPGPG
                     DRARTFYGMSPEQMRARPSAT"
     gene            complement(5547..6185)
                     /locus_tag="VFX87_00470"
     CDS             complement(5547..6185)
                     /locus_tag="VFX87_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX4993401.1"
                     /translation="MSRPAIRHLSRVDPVMRVLIRRVGPCGLAVRNHSPFETLVRAIA
                     HQQIHGRAAEAILGRFIALFPGRRFPTPDDIATLDARKMRRAGFSRAKTRSIKDIARK
                     TRSGLIPTRRACHRLTDAELIERLVQVRGVGQWTVEMLLLFTLGRPDVLPVDDFGVRE
                     GFKVAYGRRKQPTPKQLRRYGARWEPYRSVAAWYLWRAAEKALHRKIEPKVT"
     gene            6249..7184
                     /locus_tag="VFX87_00475"
     CDS             6249..7184
                     /locus_tag="VFX87_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4993402.1"
                     /translation="MQTTPVRYPDPAVQILDESFAKYRIFNAAVERLATGFRWAEGPV
                     WFGDGRYVLWSDIPNDRIMRWDEETGVVSVFRKPANYSNGNTRDRQGRLVTCEHDSRR
                     VTRTEYDGAVTVLMDSFENKRLNSPNDVVVKSDDSVWFTDPPFGVLGNYEGHTAPLEL
                     PTNVYRLDPRSGTATVATGDIPRPNGLCFSPDESRLYVVVSGAMPREIRVFDVVAGGT
                     ALANGRVFINTGAGIPDGLRCDTDGNLWCGWGGGEGLDGVAIYNPDAKVIGRILLPER
                     CANLCFGGAKRNRLFLAASQSLYSIFVNTQGVAGG"
     gene            7195..8403
                     /locus_tag="VFX87_00480"
     CDS             7195..8403
                     /locus_tag="VFX87_00480"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993403.1"
                     /translation="MALPTGLRAFRHADFRRFFAAQFVAQVGSWMQTVAQSWLVLQLT
                     SSPLLLGLIGTLQFGPILLFSIVSGAVADRVPKRRLLLVTQSVLGAQALVLGALVYGG
                     HVEYWHVAVLATGVGLANVLDGPARQSFVAEMVGRSDVGSAVSLNSACFNAARIVGPS
                     IGGVIIGRYGIAPAFVLNAMGFAIAIVMMLTLRARGLPQPRTGQGVAQEILVGIRYVR
                     RTREMLLALGLTFVVSLCVFNFTVYVPLVVRTVLGLGAEGFGFLMAALGVGAVSGALT
                     LGTLGARRPPRALMFTAAALACCGLIGLALVPAFWPAVAVLFITGFFGIIAVVSCNTT
                     MQLSAPDALRGRVMSIYTLIWGGVFPIGAFMVGAMSERWGVQRALMTNGTVGLAGVGV
                     LLGWWRLRAR"
     gene            8427..9608
                     /locus_tag="VFX87_00485"
     CDS             8427..9608
                     /locus_tag="VFX87_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4993404.1"
                     /translation="MASRPSVRVGIVGAGFVAHIHGEAYRHVRGVDVELACVTAARPE
                     RARAYAREFGVARAVADFHAVLADPDVDLVDLCVPAHHHAPMAIEAARAGKHVIVEKP
                     LTGFFGPPDTPREEMLTRARASADQVLAAAAAARVRLCYAENWVYAPPIQKARRLFTA
                     AGGPILRLVGEESHSGTHAPINKRWVTGGGGSLIGKACHPLGAALYLKADEGRRLRGV
                     PVRPVSVMAETARLADTATFRAATPRYLNIVDGADVEDWGTMLITFDDGTVAAISAAD
                     TVLGGIRNQMAIYGAKAVVLCNINPNDLVQAYAPDASVFGDEYIVEKIETKAGWTAPQ
                     PDEEWTTGYPQEIQDFVECVATGREPLCNGPLARDVTAVIYGAYLSANEGRRVDLRPY
                     L"
     gene            complement(9702..10610)
                     /locus_tag="VFX87_00490"
     CDS             complement(9702..10610)
                     /locus_tag="VFX87_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX4993405.1"
                     /translation="MSEPPAAANRTPVSLGTATPGGGFPVYGEAVAAVVNEADPTLDV
                     RLRNTKGSTENVPLLEAGALDLGLVQGEVAYEALAGIGRAPADLRVLAAMFSTPGMFV
                     VRADSPYRTIADLKGRPVAFGARGSGLIVLARYVLDGLGFERDRDFQAVFLDAAGDGP
                     RMVMDGTVAALWGGGLGWPGFTAVARGPAGARFLVPDLDGIHRIQTKHAFLQLMRVPA
                     GSYPGQTADLVSVGSWSFIMARPSLADEVAYRLARALHRGEAALAARLAQGRETTAAN
                     TVAAVARPELLHPGVRRYLVEIGLTR"
     gene            10751..12205
                     /locus_tag="VFX87_00495"
     CDS             10751..12205
                     /locus_tag="VFX87_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4993406.1"
                     /translation="MVNRRVTGKRSRIIHLINPKTDSLTTRPLYLNRALYSPLAGLLA
                     VAAGIPSDRYEVVLTDENIESIDFDLKADLVGLSAMTSYVNRGYEIADAFRARGVPVV
                     MGGVHPSFMPQEALKHCDAVVIGEAELVLDKLLADLERGAMRGTYRADRLHPMEGLAM
                     PRYDLLKKNRYVNRTFVQTSRGCHQGCTFCAEPLMNGLKFRYRPVAEVIREIENCGSR
                     MISINDADFFGTPERPKEIMRALKGRGVTWQAGVTSKLAQDDRMLELAAASGCTLLSI
                     GFESISRATLTSVHKHVNRPETFSALVEKVHSYGIMVFGLFMFGFDGDDPSVFRETAR
                     FNIEADYDACAYSVLTPYPGTLTWYELKKANRIVSFDWGKYDQANVVYRPARMTGDEL
                     RLGQNTAYETFYALSSMARRFPVRGGRRRLQWSIYNLFMKKGAATDRKDAVAAPTEAP
                     DVVPMPPILPLKREWREAVLEGVGGARPDHRHGG"
     gene            12373..14256
                     /locus_tag="VFX87_00500"
     CDS             12373..14256
                     /locus_tag="VFX87_00500"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4993407.1"
                     /translation="MTLPSGATLKVPIDWTVTAATDGVVLLDPEKAVRIDLVEVGTTG
                     EMRDAIPTAWSRRHPGFDRPELAASDSPGRRGWDLFRWSTYKTSPEESRQVSAFGARK
                     GPLAVVVLVDGPLAAVERRSSQVGIVQDSLRPAGFVRETYRGRTPRALDASRVAELKT
                     FIGRMREAADVPGVSVVLFDTSATLIEEGFGVRERGRPEPVSADSLYVIASNTKSLTT
                     LLLARLVDEGRFGWETPVTRIDPRFKLGDPDVTRRILVKHLVCACTGLPRQDFEWIFT
                     FDRSSAQGQLDVLATMKPTTEFGTLFQYSNPLASAAGYIGARLVKPDVELGQAYDEMM
                     HEKVFRPLGMGRTTFSFPEALGTDHASPHSWDMSLRNVLIEMALNHSIIPIRPAGGAW
                     SSARDYARYVRLELARGRLPDGSTFVSEKNLLARRVPQVRVGEDAWYGMGLRIQDVKD
                     IRVVSHGGSMFGYQSNFFFVPEANIGGVILTNADSGWRVARAVMRRTLEVVYDGKPEA
                     EEDLRAGVRETEAYLRGEQRDWTLPPEPSQVKRLAGFYRNAALGEIVVRPGRDEVVFQ
                     FGAWKSRMATKLNPDGTTSFVSIDPGVRGFEFDAPAAPGVYPRLRLRDSQQTYDYEAV
                     ARP"
     gene            14360..14851
                     /locus_tag="VFX87_00505"
     CDS             14360..14851
                     /locus_tag="VFX87_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4993408.1"
                     /translation="MELAKDGYRHILGGSVVFEHLPPAALDHIIARGLLIEVAPGETV
                     LTEGTRGPGLYVVLEGLVEIFLPDGAAGRTRRPTEVHLNTMGPGRCLGEYSLIDEHST
                     SAAARSMAGAKLFFLSREEFRSIAEGDARVGKVIYLNLLRFLISRLRAKDEELDLFMM
                     GSL"
     gene            14851..16131
                     /locus_tag="VFX87_00510"
     CDS             14851..16131
                     /locus_tag="VFX87_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4993409.1"
                     /translation="MSPHDLTVKLFLQLAVILAACRVCGWLGRRFLGQTQVVGEMVAG
                     VLLGPSLFGLLVPETQAWLFPKTLPAGGPHPSMLILYALSQIGLVLYMFLIGMEFNHR
                     LMVGRVRHASLVSGVGIVVPMALAAVAALTLRGDADLFAPHVTPGAAALYLGAAMSIT
                     AFPVLARILQEKGIARTRLGTLALAAGSSNDAAAWCLLAVVLSYLEGSWTIAVLAIGG
                     GALYVALMLLLGRPLLRVLDHGVDGRGRIAPGTLVAGLLVVMLGAFVTDVVGIYEVFG
                     AFIAGIAMPRGRFADAAIEHLEPLTTTLLLPVFFVYSGLNTQIALLDSPAIWRVAILL
                     ITLSIVGKGVGCLLAARVSGEAWRESVAIGVLMNARGLMELIILNIGLQRGIITPTLF
                     TIMVLMAVVTTLMASPLFELVYGRRAAARQEQPA"
     gene            16128..16844
                     /locus_tag="VFX87_00515"
     CDS             16128..16844
                     /locus_tag="VFX87_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4993410.1"
                     /translation="MSSRRVWSRAVLVAMALGSAACAPMGPGGHHARTSTVTVAGTGT
                     VSAAPDMAEITTGVVTQAPTAAQALAANSQAMERLLQALGTLGVAARDIQTTNISVSP
                     LRRQGRDGQPPEITGYEVTNQVRVKVRDLSRLGRVLDQQVGQGANLVYGIQFGRQEPA
                     PLLDEARKRAMADARRKAELYSAAVALKVGRVVAVQEAGAASPRPEMAPRVTMSAAVP
                     VAPGEQEIQASVTVTFTLEP"
     gene            16852..18303
                     /locus_tag="VFX87_00520"
     CDS             16852..18303
                     /locus_tag="VFX87_00520"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4993411.1"
                     /translation="MTAPHFLSAKQLASNIRRRKIGCLEALDLFLARVDRFNPELNAI
                     VASDVAAARRRARQADAALQKGKVWGPLHGVPMTVKESYDVVGMPTTWGLSELKNNMP
                     PRNALAVDRLLGAGVVLFGKTNVPSWLADWQSFNPVYGTTNNPWDLSRTPGGSSGGSA
                     AALAAGLTGFEAGSDIGASIRNPAHYCGVYGHKPTYGIVPPRGQALPGRVAQGDISVV
                     GPMGRSADDLALGLSVMAGADEIDRAGWRLALPAPRRKALREFKVAVMRTDPVSEVDD
                     EVQARVQAVADFLAGKKAKVSDTARPAIDTAEAQRVYVFLLRAATAGRQTSEQFGRNA
                     EIARGLRPDDESYYARMTRANVAPHRDWLAANEARHQMRWRWAEFFRDYDLLLCPAAA
                     SPAFPHDHAGERHERTIAVNGRRVPTTDQLFWAGYPGVAFLPSTVAPAGFSRAGLPIG
                     VQIVGPQYGDRTCIELARLLEREFQGFVPPPGY"
     gene            18314..19177
                     /locus_tag="VFX87_00525"
     CDS             18314..19177
                     /locus_tag="VFX87_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993412.1"
                     /translation="MAKKRVVVTGAAGYVAQRMLPELRERWDLVLLDVRPTTRDGKTV
                     PGLMVADLTKPDRNEYRQHFRGADAVIHCGFVSAPRLDATTWQDNSDAKFWAEHTNVA
                     LAYNVCRVALEEGVRRVVVCSSNHAADYYERLVWDGRLDMVTPEMPPRSDNWYGWAKA
                     AYELLGFVFATGKVDGRKLEIVQWRIGGPRDDDIDQVKPGDIKVMHRALGAYLSKRDQ
                     VQQAVRMVETEDIRDEHGVPFLIVYGISGNTHRFWSIASARVKLGYAPEDDSQVNFAD
                     KIAAIARAARR"
     gene            19180..19377
                     /locus_tag="VFX87_00530"
     CDS             19180..19377
                     /locus_tag="VFX87_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993413.1"
                     /translation="MNAEEELKALEGTLVTFRETLKEVNRLSGDGMVAVREEWLLRIK
                     ELELQREHLSHVVRKNRRRVG"
     gene            19411..19716
                     /locus_tag="VFX87_00535"
     CDS             19411..19716
                     /locus_tag="VFX87_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX4993414.1"
                     /translation="MRGDRVARELRFVQVDVFTQRVSGVPEDPATGSASGPLGAYLVE
                     RGLVAPADTVDIVSEQGTRMGRPGFIRIRVDMRAGRVSEILVGGRVVPVIESRLRVP"
     gene            19721..20350
                     /locus_tag="VFX87_00540"
     CDS             19721..20350
                     /locus_tag="VFX87_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX4993415.1"
                     /translation="MYIAKQFQITDTAWCHTLMRVQSFATMVTTDDTGVPFATHLPIL
                     VDPARSPLGTLRGHVARANPHWRYLSAGRPTLVVFCGAHAYVSPSWYATHPSVPTWNY
                     VAVHATGTGTLVEDPEQVRTLLTDLVRTYESGGPTAWSLEGLSADYLAGMQRGIVAFE
                     IPIERLEGKAKLSQNRDAVDQARTREALAASDDPLARAVAALMAGAPPR"
     gene            complement(20354..21124)
                     /locus_tag="VFX87_00545"
     CDS             complement(20354..21124)
                     /locus_tag="VFX87_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4993416.1"
                     /translation="MADEILLSVDSGIATVTMNRPDQRNAMNTPLLQALRATFDDLDG
                     RRDVRVVVVRGAGPAFCAGMDLKEMQQRRGEADPEGNVVDVLRRVERSKHPTIAALHG
                     DAIAGGCELALHCDLRVAAEPARLGMPLARIGLVIPFPLGQKLVEIIGPAQTRQLLFT
                     GQPIDARRAYEIGMVHQVVPADDLSAAVQALARRIADNAPLSLAGIKASILRATSTRE
                     RIAHDDLDAAATRARTSADAQEGRRAMLEKRKPVFRGE"
     gene            complement(21139..>21705)
                     /locus_tag="VFX87_00550"
     CDS             complement(21139..>21705)
                     /locus_tag="VFX87_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993417.1"
                     /translation="TKVPALPVPIHIAALREGMLRVAGQVGDGVILNWLSPDDVPRSV
                     AVVREAAKRAGRDPEAIEVTARLFVNLDPPGPESDLVVRRHIAGYLNVPVYRAFQEWL
                     GRTEALGPMWAAWGAGDRKGAVAAIPTRVMDDLIPRGSLAELKARIQRYLDRGIDTAF
                     LSFMTTEQDPKKKRDLMLNALRVMAPRP"
BASE COUNT         2961 a         7679 c         7804 g         3261 t
ORIGIN      
        1 ggcagcggca ccccgtggac accggacggc gccgtgtcag cagacagccc gaatccgtca
       61 cctcgcgcgc gcccggccct cgcaattcca ggcacttggc cggtcggccc gggtggcaag
      121 aatgttgccg tctggaatcg ccatgctggc cataccgccg gggctcatgc gctggatctt
      181 cgggatcgtg ctggccgctt ccctgctggc gatgggctgc aatggcccgg actcgagagc
      241 caggggctcg atcaaggccg gggatgaggc ggcccagcaa cgggccctgc gcgaggcgct
      301 gaaccactac cggaccgccg cggtggcgga gcccgcctct ctcgaggccc agacgcgccg
      361 cggcgcgatg gccgagctgc tgggcgagtt cgacgaggcg ctcgacgcct acagccgcgc
      421 gagccgcctt cagccctcgg gcctggccta ctaccgcgcc ggtgccatgg ccgatcgcat
      481 gggcaatacc gtgctcgccg ccgagtacct gaacgccagc ctgcaggcgc cgcccacccg
      541 cggcgagcgg tgggcgcaga ccggtcagca cgcgatcgag cgggtgaccc tctcgctcaa
      601 cggctcgcgc tggggccgca tgctgcccga ctgggtcttc cgctcgctcc acgcgacccg
      661 cgtggccctg gccaatgccg tgctcgatcg cgaggtggtg gcggcggcgc tcttcacctc
      721 gttgctcgag gcgggcgaga acgagcgggc gctcgaggtg gcgcgcgggc gcggctgggt
      781 gcgcgcgggc gccgactact gcgcggccgg gcgcggagtg gtgggggccg agacgcgagc
      841 cctggtcggc atgctcgccg cgccggagcg cgcggactgc ctccttccgc tggggcgtgc
      901 gctgaccgat gccggtctgg tccggctctc gcggctggtg ctgcaggacc ggatccgtcg
      961 cgcgccggat ctgcgcgcgc gccgcgaggc ggaggccttc ctgcgctacc gcctgcccgc
     1021 gcacgacgtg cccaagaccg ccgagtcgct caacatcgcg gcctacaatc tgcagcaccg
     1081 cttcggcgat cagggcgcgg cggcggccgc gtaccagaag gcgatcgccg ccgacccgaa
     1141 gttctcctgg ccctacgcca atctcggccg cctgtacatg gagctcgatc agtacgatct
     1201 ggcggtggac tggctcggca acgcgatccg catcaacccg aatcacttcc gcgcccacgc
     1261 caacctgggc gtggcgctgc agaccctgcg acggtacgac gaggcggtgg gggcctaccg
     1321 cgccgcgctc gcgctcaatc cggacgacgc ggccacccac gcgaacctgg ggcgcagcct
     1381 gctcagcctg ggtcgcgacg cggaaggcct gcgctcgctg cagaccgcgg tgcgtctcga
     1441 cccgaggctc gaggaggagc gtgagctcct gacccggcgc ctggccggcg gcttccgcct
     1501 ggactgacgc cgcggtatgg tggggcgccc atgcgccccg acccgacggc ctcccccgcc
     1561 ccttcgtatc tcggcctcgg cgtcaccgtc gtcaccctcg cgctggccta cggggtctgg
     1621 tacgcctaca gcgtcttcct ggtcgcgctg ctccgggagt tcggctggag ccgctcggtg
     1681 ctggccggcg ccttctccat cttcacgctg gtcagcggcg gcgccggccc cgtgctgggc
     1741 gccctcgccg atcgcttcgg gccgcgccgg ctcatcctga tcggcggcgt gctgctggcg
     1801 ggctcgctct gggcggacag cctggtcacg cgcgcctggc atctctacct gaccttcggc
     1861 ctgctcaccg ccgtcggggt ggccaccgcg ggctggacgc ccgcggtggt gatggtgcag
     1921 cggcagtgga aggcgcgtct gggcctggcg ctcggcatcg ccggttccgg ggtgggcctc
     1981 ggcatcttcc tggtcgtccc gctctgccag gcgctgatcg acggcttcgg ctggcgctgg
     2041 gcgttccgcg cgctcgccat cctctgcgcg ctctggatcc tgcccgcgac cttcctggcc
     2101 atccgcgaca cgccaccgtc gtcgccccgg gagcccgctg cgcgcggcga gacgggccgg
     2161 ccggcgggcg gcgagcattc cctcggcctg gcgctggcca atccgtcctt tcgactcatt
     2221 ggtcttgccg tcttcctcgg cagcatctgc agccagacgc tccacgtcca ccaggccgcc
     2281 ttcctcgtgg accacggcct gccggccatg accgccgcct cggtgatcag cgcggtgggc
     2341 gcctccagca tcgtgggcaa gacgggcggg ggctggatct cggatcatct ctcgcgcgag
     2401 ctcgtctacg cgctcggcat gatcggcatg atattcggcg tgggtgtgct ctggctggtg
     2461 gccctggccc cgtcggcgtg gctggcgctc ggctacgcgg tgctgttcgg tgtgggctac
     2521 tcggtcaccg cgttcatcgt ccccgccatg atgagcgacc gcttccgcgg cccgcacttc
     2581 ggctcgatct tcggcgcgac ccaggtggcg agcgcgctgg gcagcgccct gggggcgtgg
     2641 ctggccgggc ggatcttcga cgcgacggga agctacgcga tcgcgttctc gctggccgcg
     2701 gcggccgccg gggtggccgc gctgtcggta tgggcgggtc ggttgcgcgg gccggcgcgg
     2761 ccctaggggc tctcgccgcg cgcgggcgcg acgacgacga cgtccgacgc ctggagcgcc
     2821 cggcgctcgc ggacgggccg cggccgggga gcggtcgccg cgtggtcctc tgccgagagg
     2881 acgcgcgcgg gctcggggac ctcgggcgcg cggacgcggg ccggagccgg cgcgggggcc
     2941 gcgtcacgcg ccgcggcctt gacgggcgca ggcgcgggct cgaccgtggc gcgcggcgcc
     3001 gcgggggccg ggcgcgcgga cgcggccgcg ccgggcggcg gatcggcatt cggaccggag
     3061 gcatcgagat gcaggagata cggcagcgag acgagggcga ccaggccgac cgcggtcagc
     3121 agcagcgagg cgcgcaccca gccgcgcgcc agccagccat gctcgtcctc gtcctggccg
     3181 gtgagccagc ggatctcctc ctgcagcgcg gccacttctg cgcgcagctg cgtggcctcg
     3241 tcctcgaggc tcacgagccg ttgatccacc gcgctgatgt tgcgctccgg tgaggccatg
     3301 atccccgaga gggagcaaga ggggggccag cccgtcgatg gctgaaaagt cggcagctta
     3361 tgcggcgggg gcgcatcttc ttcggcgcga ttcgcgtcag cgcggcgtcc ggcgatgacg
     3421 attccggaca tcctggcgct cgatttcgac ggcgtttgct gcgacggcct gcgcgagtac
     3481 ttcgagacga gtcgccgcac ctgggcgcgc gtgtggcccg cggacgagcc gcccgccgac
     3541 agcgccttcg tggcgttctg cgcgctgcgc ccggtgatcc tgagtggctg ggagatgccg
     3601 gtgctcctgc gcgccatcgc gcgaggcgtg ccgcgcgcgg agatcctggg cggctgggag
     3661 cgggtacgcg cggagctcgt cgcgacgggg tcgccgagcg gcgacgcgct gatcgcgcgc
     3721 ctcgcccacg cgctcgacga ggtgcggcgc gagtggatcg ccgccgacga gccgggctgg
     3781 ctcgcgctcc atgctccgta cggcagcgtg gacgagctgc gccgcctcgt ggccgggccc
     3841 gggcgcagcg tgctggtcac caccaaggag ggtgcgttcg cgcggcgcat cctcgattcc
     3901 tggggcgtgc ggctcgcgga cgtgcagggc aaggagaccg gcgcgcacaa gtgcctgaat
     3961 ctccgcgcgc tgatcgcggc gtggaccgcc gagcgcggga gccggccccg gctggccttc
     4021 gtggatgacc gcctcgagac gctccggcac gtgaccacgc acccggatct ggacgacgtg
     4081 gcgctcttcc tcgccgcctg gggctacaac acggaggcgg cacgggcggc cgcgcgcgcg
     4141 gatggaagga tccgcctcct caccctcgag cagttccgtc ggggcgccgc cgcctggtcg
     4201 tagccggcat tccggcagcc gctcggcccg ccttcggctc acttcgcccg gccgcggctg
     4261 cgcctcgctg tgctcttgtt cgcgcccgcc ggctgtggca tggtctgtcg ccatgaagat
     4321 cgcgcgcgtg gaagccctgc actgtgacgg cggctggcgg ccctggacct tcgttcgcgt
     4381 cgagaccgac gacggcctcg tgggctgggg agagtgcagc gacaatcgca gcccgtacgg
     4441 catcgcgggc tccgttcgcg acctcgcccc gctgctgatc ggccaggacc cgcggccggt
     4501 cgagcgcctg tactgggaca tgctccgcgc cacgcgccag aacctgggcg gcgtgaccca
     4561 caaggcgatg gcgggcatcg agctggccct gtgggacatc aaggccaagg ccctcggggt
     4621 gccggtctac gagctcttcg gcggcccgct gcgcgaccgc atgcggctgt actggtcgca
     4681 ctgcggaacg acgcgggcgc ggctcggcca cgtgctgggc accccgccgc tgcgcaccta
     4741 cgacgacatc gccgcgctcg gcaaggaagt ggtggcgcgc ggcttcaccg cgctcaagac
     4801 caacatggtg atcccgggcg atcccgccac cgtgtacttc ccgggtttcg cgagcgggat
     4861 caactcgacg gacggcgccc ccaccgtgga gatcctgggg gccatcgagc gcctgatcgg
     4921 cacgttccgc gacgcggtgg ggcccaaggt cggcctctgc ctcgacctca actacaactt
     4981 ccgcaccgag ggcgtgctgc gggtggccaa gctgctcgag cagttcgaca tgcagtgggt
     5041 cgagtacgac aactgggacc cgcaggccct gctgcagatc aagcaatcga cgtccacacg
     5101 gctcgcttcg ctcgagagtc tggtgaccac gcgccagtac cggcccttcc tcgagctgca
     5161 cgcggtggac gtggccatca tcgacgtgcc gtggaacggc ttctcccagg ccgtgcagat
     5221 cgggcggatg gccgaggcct acgagatcaa catcgcgccc cacaactact acagccacct
     5281 ggccgatctc cattcgctgc atctgtgcgc ggtgctgccc aacgtgcgca tcatggagat
     5341 cgacatcgac gacgtgtcct ggaaggcgga cctcgtgacg aagccgcccg tcgtcaagga
     5401 cggccacgtc ttcctgagcg acgcgccggg ctggggcgcc gacatcaacg aggaggtgct
     5461 tcgcgcgcat ccctggccgg ggccgggcga ccgcgcgcga acgttctacg gcatgagccc
     5521 cgagcagatg cgagcgcgcc cgtccgctac gtgaccttcg gctcgatctt ccggtgcagg
     5581 gccttctcgg ccgcgcgcca gagataccag gcggccaccg agcggtaggg ttcccagcgc
     5641 gcgccgtagc gccggagctg cttgggcgtc ggctgcttgc gccggccgta ggcgaccttg
     5701 aagccctcgc gaaccccgaa gtcgtccacc ggcagcacgt cgggccggcc gagagtgaag
     5761 agcagcagca tctccaccgt ccactgcccg accccgcgca cctggaccag ccgctcgatg
     5821 agctcggcgt cggtgaggcg gtgacaggcg cgccgcgtgg ggatcaggcc gctgcgcgtc
     5881 ttccgcgcga tgtccttgat cgagcgcgtc ttcgcccgcg agaacccggc gcggcgcatc
     5941 ttgcgcgcgt ccagcgtggc gatgtcgtcg ggcgtcggaa agcgccggcc cgggaagagc
     6001 gcgatgaagc gccccaggat cgcctcggcg gcccgcccgt ggatctgctg atgcgcgatg
     6061 gcccgcacga gcgtctcgaa gggcgagtga ttccgcaccg cgaggccgca gggccccacg
     6121 cgccggatga gcacccgcat caccgggtcc accctggaga gatgccggat cgcgggacgg
     6181 ctcatgtcgc gcgactgtag cacaaggtta ttgacttctc tgccgtgagt ggccaccatc
     6241 cgggggtcat gcagaccacg cccgtgcgtt atcccgatcc ggcggtgcag attctcgacg
     6301 agagcttcgc caagtaccgc atcttcaacg cggcggtgga gcgcctggcc accgggttcc
     6361 gctgggcgga ggggccggtg tggttcggcg atggccgcta cgtcctgtgg agcgacatcc
     6421 ccaacgatcg catcatgcgc tgggacgagg aaaccggcgt ggtctccgtc ttccggaagc
     6481 ccgcgaacta ctcgaacggc aacacgcgcg atcgccaggg gcggctcgtc acctgcgagc
     6541 acgacagccg gcgcgtgacc cgcaccgagt acgacggcgc ggtcacggtg ttgatggact
     6601 cgttcgagaa caaacggctc aactcgccga acgatgtggt ggtcaagtcc gacgacagcg
     6661 tgtggttcac cgatccgccc ttcggcgtgc tcggcaacta cgagggtcac accgcgccgc
     6721 tcgagctgcc caccaacgtg taccgcctgg acccccggag cggcacggcc accgtggcga
     6781 ccggcgatat cccgcgcccc aacggcctct gtttctcgcc cgacgagagc cggctctacg
     6841 tggtggtgtc gggagcgatg ccgcgcgaga tccgcgtgtt cgacgtggtc gcgggcggca
     6901 ccgcgctggc caacggtcgc gtcttcatca acaccggagc gggcatcccg gacggcctgc
     6961 gctgcgacac cgacggcaac ctctggtgcg gctggggcgg gggcgaaggg ctcgacggcg
     7021 tcgcgatcta caatcccgac gccaaggtca tcggccgcat cctgctgccg gagcgctgcg
     7081 ccaacctctg cttcggcggg gccaagcgca accggctctt ccttgccgcg agccagtcgc
     7141 tctactcgat cttcgtcaat acccagggcg tggccggcgg ctgagcggcc gccgatggcc
     7201 ctccccaccg gcctgcgggc gttccgccac gccgatttcc ggcggttctt cgccgcgcag
     7261 ttcgtggccc aagtcggctc ctggatgcag acggtggcgc agtcctggct ggtgctccag
     7321 ctcacctcgt ccccgctgct cctcggcctg atcggtacgc tccagttcgg gccgatcctc
     7381 ctcttctcga tcgtgtcggg cgcggtcgcc gatcgcgtgc ccaagcggcg gctgctgctc
     7441 gtgacccagt ccgtgctcgg cgcccaggcg ctcgtgctcg gcgccctggt gtacgggggc
     7501 cacgtcgagt actggcacgt ggcggtcctc gccaccgggg tcgggctcgc caacgtgctc
     7561 gacgggccgg cccgccagtc gttcgtggcc gagatggtgg ggcgttccga cgtgggcagc
     7621 gcggtgtcgc tcaactcggc gtgcttcaac gcggcccgca tcgtggggcc gagcatcggg
     7681 ggcgtcatca tcggccgcta cgggatcgcg cccgccttcg tgctcaacgc gatgggcttc
     7741 gccatcgcga tcgtgatgat gctcaccctg cgggcccgcg ggcttccgca gccgcgcacc
     7801 ggccagggcg tggcccagga gatcctcgtg ggcatccgct acgtccggcg gactcgcgag
     7861 atgctgctgg cgctcgggct caccttcgtg gtgagcctct gcgtgttcaa cttcacggtc
     7921 tacgtcccgc tggtggtgcg caccgtgctg ggcctcggcg cggaggggtt cggcttcctc
     7981 atggccgcgc tcggggtggg ggcggtgagc ggggcgctca ccctcggcac cctcggggcc
     8041 cgtcgcccgc cgcgcgcgct catgttcacg gcggccgcgc tggcctgctg cgggctcatc
     8101 ggtctcgcgc tggtgcccgc gttctggccc gcggtggcgg tactgttcat cacgggattc
     8161 ttcggcatca tcgcggtggt ctcctgcaac acgacgatgc agctctccgc cccggacgcg
     8221 ctgcgtggcc gggtgatgag catctacacg ctgatctggg gcggcgtgtt cccgatcggc
     8281 gcgttcatgg tgggggcgat gtccgagcgc tggggcgtcc agcgcgcgct gatgacgaac
     8341 ggaaccgtgg gcctggccgg ggtcggggtg ctgctcggct ggtggcggct gcgggcgcgc
     8401 tgagcgccgg gaggagagag atcgtgatgg cgtcacgacc gagcgtgcgg gtgggcatcg
     8461 tgggcgcggg cttcgtcgcc cacatccacg gcgaagcgta tcgtcacgtc cgcggggtgg
     8521 acgtcgagct cgcctgcgtg accgcggcgc ggcccgagcg cgcccgggcc tacgcgcggg
     8581 agttcggcgt cgcgcgcgcg gtcgcggatt tccacgcggt gctcgccgat ccggacgtcg
     8641 acctggtgga tctctgcgtg ccggcccacc accacgcgcc gatggcgatc gaggccgccc
     8701 gcgcgggcaa gcacgtgatc gtggagaagc cgctcaccgg cttcttcggc ccgccggaca
     8761 ccccgcgcga ggagatgctg acgcgcgcgc gggccagcgc cgaccaggtg ctggccgccg
     8821 cggcggcggc gcgcgtgcgg ctctgctacg cggagaactg ggtctacgcc ccgccgatcc
     8881 agaaggcgcg ccggctcttc accgccgcgg gcgggccgat cttgagattg gtcggcgagg
     8941 agagccactc gggcacgcac gcgccgatca acaagcgctg ggtcaccggc ggcggcggct
     9001 cgctgatcgg caaggcctgc catccgctgg gcgccgcgct ctacctgaag gcggacgagg
     9061 gcaggcgcct gcgcggcgtc ccggtccggc cggtctcggt gatggcggag accgcccggc
     9121 tcgccgacac cgcgaccttt cgcgcggcca cgccgcgcta tctcaacatc gtggacggcg
     9181 ccgacgtgga ggactggggc accatgctga tcaccttcga cgacggcacg gtggcggcga
     9241 tctcggcggc cgacacggtc ctgggcggca tccgcaacca gatggcgatc tacggcgcga
     9301 aggcggtggt gctctgcaac atcaatccca acgacctcgt gcaggcctac gcgcccgacg
     9361 cgtccgtgtt cggcgacgag tacatcgtgg agaaaatcga gacgaaggca ggctggaccg
     9421 cgccccagcc cgacgaggag tggaccaccg gctacccgca ggagatccag gacttcgtgg
     9481 agtgcgtggc cacgggccgc gagccgctct gcaacggccc gctcgcccgc gacgtcaccg
     9541 cggtcatcta cggcgcctac ctctccgcga acgaaggccg ccgcgtcgac ctccggccct
     9601 acctctgacg gggtcaggtc ttacattccg acatttccgc cgcgggccgg cctccgccac
     9661 ggccagaaga atgtcggaat gtaagacctg accccaagcg gtcagcgcgt gaggccgatc
     9721 tccacgaggt agcgccgcac gcccgggtgc agcagctccg gccgcgcgac cgcggccacc
     9781 gtattggcgg cggtggtctc acgcccctgc gcgagccgcg ccgcgagcgc agcctcgccg
     9841 cggtggagcg cgcgcgcgag gcggtaggcc acctcgtcgg ccagcgaggg ccgcgccatg
     9901 atgaagctcc acgagcccac cgagaccagg tccgcggtct gccccgggta ggagccggcg
     9961 ggcacgcgca tgagctggag gaacgcatgc ttggtctgga tgcggtggat gccgtcgaga
    10021 tcgggcacca ggaagcgcgc gccggcgggc ccgcgggcca ccgcggtgaa gccgggccac
    10081 cccagcccgc caccccacag ggcggccacc gtgccgtcca tcaccatgcg ggggccgtcg
    10141 cccgccgcgt cgaggaagac ggcctggaag tcgcggtccc gctcgaagcc cagcccgtcg
    10201 agcacgtagc gcgcgaggac gatcaggccc gagccgcgcg cgccgaaggc caccggcctg
    10261 cccttgagat cggcgatggt gcggtagggc gagtcggctc gcaccacgaa catgccgggc
    10321 gtggagaaca tcgcggccag cacgcgcagg tcggcgggcg cgcggccgat cccggcgagc
    10381 gcctcgtacg cgacctcgcc ctgcaccagc ccgagatcga gcgcgcccgc ctcgagcagg
    10441 ggcacgttct cggtgctgcc cttcgtgttg cggagccgca cgtcgagcgt ggggtccgcc
    10501 tcgttgacca cggcggcgac cgcctcgccg tagaccggaa atccgccgcc cggcgtggca
    10561 gtgccgaggc tcaccggcgt ccggttcgcg gcggcaggcg gctcgctcac ggcgcgtacc
    10621 gtaccacatc gctctcccgc gggagtagtc cgccttgacg cctccgggcg cgagtcttac
    10681 catttcgcca ttcgacccgc tggactgtcg actcggcaac ctgttcgacc ctggtaagga
    10741 ggagcgggcc atggtcaatc gtcgggtgac cggcaagcgg tcgcgcatca tccacctcat
    10801 caatccgaag acggattcgc tcaccacccg gcctctctac ctgaaccggg ctctctattc
    10861 gccgctcgcg ggcctgctgg cggtcgcggc gggcatcccc agcgaccggt acgaggtcgt
    10921 gctgaccgac gagaacatcg agtcgatcga cttcgacctg aaggcggacc tggtcggcct
    10981 gtccgcgatg acgagctacg tgaaccgcgg gtacgagatc gccgatgcct tccgggcgcg
    11041 cggggtaccc gtcgtgatgg gcggcgtgca ccccagcttc atgccccagg aggcgctcaa
    11101 gcactgcgat gcggtggtga tcggagaggc ggagctcgtg ctcgacaagc tcctcgccga
    11161 tctcgagcgg ggcgcgatgc gcggtaccta ccgggcggac cggctgcacc cgatggaagg
    11221 cctcgcgatg ccgcggtacg acctgctcaa gaagaaccgc tacgtgaacc gcaccttcgt
    11281 gcagacctcg cgcggctgcc accagggctg cacgttctgc gccgaaccgt tgatgaacgg
    11341 gctgaagttc cgctatcggc cggtcgcgga ggtgatccgg gagatcgaga actgcggctc
    11401 gcgcatgatc tcgatcaacg acgccgactt cttcggcaca ccggagcgcc cgaaggagat
    11461 catgcgggcg ctgaagggcc gcggggtcac ctggcaggcc ggcgtcacct cgaagctcgc
    11521 gcaggacgat cggatgctcg agctcgcggc cgcgagcggc tgcaccttgc tgagcatcgg
    11581 gttcgagtcg atttcacgcg ccacgctgac cagcgtgcac aagcacgtga accgccccga
    11641 gaccttctcc gcgctcgtgg agaaagtcca ctcgtacggc atcatggtgt tcggactgtt
    11701 catgttcggc ttcgacggcg acgacccttc ggtgttccgc gagacggcac ggttcaatat
    11761 cgaggccgac tacgacgcct gcgcgtactc tgtcctcacg ccgtatccgg gcacgctgac
    11821 ctggtacgag ctgaagaagg ccaaccgcat cgtctccttc gactggggaa agtacgatca
    11881 ggccaacgtc gtgtatcggc ccgcgcggat gaccggcgac gagctgcggc tgggtcagaa
    11941 cacggcctac gagacgttct acgcgctctc gtcgatggcc cggcgctttc cggtccgcgg
    12001 cggtcgccgc cggctccagt ggtccatcta caacctgttc atgaagaagg gggccgcgac
    12061 cgatcgcaag gatgcggtcg ccgcgccgac cgaggcgccc gacgtggtgc cgatgccgcc
    12121 cattcttccg ctcaagcgag aatggcgtga ggcggtgctg gaaggggtgg gaggcgcgcg
    12181 cccggatcat cggcacggtg gctagccgat gcggcgcttg aggacaccgt gacgcgacga
    12241 cacggcccac acgcgcctcc gtcccggagg gtcggtctca ccgccttggt gctccttgcg
    12301 gccggcgtgc tggccggctg cgcggcgcct ccctccgatc gatcggcgga gactccgcgg
    12361 gcggaccggg cgttgacgct tccgtccggc gccacgctca aggtcccgat cgactggacg
    12421 gtgacggccg cgacggacgg cgtggtcctc ctggatcccg agaaggcagt gaggatcgat
    12481 ctcgtcgagg tcggcacgac cggcgagatg agggatgcca tccccaccgc ctggtcgcgc
    12541 cggcatccgg gcttcgaccg gccggagctc gccgcctccg attcgccggg gcgccggggc
    12601 tgggacctat tccgctggtc cacgtacaag acgtctccgg aggaatcgcg gcaggtgtcg
    12661 gcgttcggcg cgaggaaggg cccgctcgcg gtggtcgtcc tggtcgatgg gcccctcgcg
    12721 gcggtcgagc ggcgctcttc acaggtcggg atcgtgcagg acagcctgcg cccggccggc
    12781 ttcgtccgcg aaacgtaccg gggccgaacc ccgcgggcgc tcgacgcgtc acgcgtcgcg
    12841 gagctgaaga cgttcatcgg gcggatgcgc gaagcggccg acgtgcccgg ggtgtccgtc
    12901 gtgctcttcg acacgagcgc gacgctcatc gaagagggct tcggggttcg ggagcggggc
    12961 cggcccgagc cggtgagcgc ggacagcctg tacgtcatcg cgtcgaacac caagtcgctg
    13021 accacgcttc tgctggcccg cctcgtcgac gagggccgct ttggctggga gacgccggtg
    13081 acgcggatcg acccgcgctt caagctcggg gaccccgacg tcacccgacg catcctcgtg
    13141 aagcacctcg tctgcgcgtg taccggcctg ccccgacagg acttcgagtg gatcttcacg
    13201 ttcgaccgct cgtcggcgca gggccagctc gacgtgctcg cgaccatgaa gcccaccacc
    13261 gagttcggaa ctctcttcca gtacagcaat ccgctggcct cggcggcggg ctacatcggc
    13321 gcgcgcctgg tcaagcccga cgtggagctg ggacaggcct acgacgagat gatgcacgag
    13381 aaggtcttcc ggccgctcgg gatgggccgc acgacgttct ccttcccgga ggcgctcggc
    13441 acggaccacg cctcgccgca ctcctgggac atgtcgctgc ggaacgtgct catcgagatg
    13501 gcgctgaacc actccatcat tccgatccgt ccggcgggcg gggcctggtc gagtgcgcgg
    13561 gactacgcgc gctacgtgcg cctcgaactc gctcggggcc ggcttccgga cggctcgacg
    13621 ttcgtcagcg agaagaacct gctcgcacgc cgcgtcccgc aggtgcgggt cggtgaggac
    13681 gcttggtacg gcatggggct ccggatccag gacgtcaagg acattcgggt cgtcagccac
    13741 ggcggctcga tgttcggcta ccagagtaat ttcttcttcg tgccggaggc caacatcggc
    13801 ggggtgatcc tcacgaacgc ggattccggc tggagggtag cgagggcggt catgcggcgg
    13861 actctggaag tggtctacga cggcaaaccg gaggcggagg aggatctgcg ggcgggggta
    13921 cgggagaccg aggcgtacct gaggggcgag cagcgggact ggacgctgcc tcccgagccc
    13981 tcccaggtga agcgcctcgc gggtttctac cggaacgcgg cgctcggcga gatcgtcgtg
    14041 cggccgggcc gggacgaggt cgtgttccag ttcggggcat ggaagagtcg catggccacc
    14101 aagctgaacc cggacggcac gacgtcgttc gtctcgatcg accccggtgt ccgcggcttc
    14161 gagttcgacg cgcccgccgc ccccggcgtc tacccgcgac tgcggctgcg ggactcacag
    14221 caaacctacg actacgaggc ggtggcgcgc ccatgatccg cataccgccc cgcccccgtg
    14281 gccgagatca cggccagcgc cgagatttgc cgtggttggt cctggacaag gcaggagtct
    14341 gtgccagaat gggcccctca tggagctggc caaggacggc taccggcaca ttctcggggg
    14401 ctcggtggtg ttcgagcacc tcccgccggc cgccctcgat cacatcatcg cgcgcgggtt
    14461 gttgatcgag gtggcccccg gcgagacggt cctcaccgaa ggcacgcgcg gccccggcct
    14521 ctacgtggtg ctggaaggcc tcgtggagat cttcctgccc gacggcgccg ccggccgtac
    14581 ccggcgcccc accgaggtgc acctcaacac gatgggcccc ggccgctgcc tcggcgagta
    14641 ctcgctgatc gacgagcact ccacctcggc ggcggcgcgc tccatggccg gcgccaagct
    14701 cttcttcctc tcgcgcgagg agttccgcag catcgcggaa ggcgacgcgc gggtgggcaa
    14761 ggtgatctac ctgaacctgc tccgcttcct gatctcgcga ctgcgcgcca aggacgagga
    14821 gctggatctg ttcatgatgg gctcgctctg atgagcccgc acgatctcac cgtgaagctc
    14881 ttcctgcagc tcgcggtgat cctggccgcc tgccgggtct gcggctggct cgggcgccgc
    14941 ttcctaggcc agacccaggt ggtgggcgag atggtggcgg gcgtgctgct cggaccgtcg
    15001 ctgttcgggt tgctcgtgcc cgagacgcag gcctggctct tcccgaagac gcttcccgcg
    15061 ggcgggccgc acccgtccat gctcatcctc tacgcgctca gccagatcgg tctcgtgctc
    15121 tacatgttcc tgatcggcat ggagttcaac catcggctca tggtgggccg cgtgcgccac
    15181 gcctccctcg tctccggcgt ggggatcgtg gtgcccatgg cactcgccgc cgtcgccgcg
    15241 ctcacgctgc ggggcgacgc ggacctgttc gcgccccacg tgaccccggg ggcggccgcc
    15301 ctgtacctgg gcgcggccat gtccatcacc gcgttcccgg tgctcgcgcg gatcctccag
    15361 gagaagggca tcgcgcgcac ccgcctgggc accctggcgc tcgcggcggg ctcgagcaac
    15421 gacgcggcgg cctggtgcct gctcgcggtg gtgctctcct acctcgaggg ctcgtggacg
    15481 atcgcggtcc tcgccatcgg gggcggtgcg ctctacgtcg cgctcatgct cctgctcggg
    15541 cggccgctcc tgcgggtcct ggaccacggc gtggacggcc ggggccggat cgcgcccggg
    15601 accctggtgg ccggcctgct cgtggtcatg ctgggggcat tcgtcaccga cgtggtgggg
    15661 atctacgagg tcttcggcgc cttcatcgcg ggcatcgcga tgccacgggg ccgcttcgcg
    15721 gacgcggcga tcgagcatct ggagccgctc accacgacgc tcctgctgcc ggtcttcttc
    15781 gtgtactcgg gcctcaacac ccagatcgcc ttgctcgact cgcccgcgat ctggcgggtc
    15841 gcgatcctcc tgatcactct ctcgatcgtg ggcaagggcg tggggtgcct gctcgcggcg
    15901 cgcgtgtcgg gcgaggcctg gcgcgagtcg gtcgcgatcg gcgtactgat gaacgcgcgc
    15961 gggctcatgg agctgatcat cctcaacatc gggctgcaga gggggatcat caccccgacg
    16021 ctgttcacca tcatggtgct gatggcggtc gtcaccaccc tgatggcgtc cccgctgttc
    16081 gagttggttt acggccggcg cgccgcggcc cggcaggagc agcccgcgtg agctcccggc
    16141 gggtctggtc tcgggcggtg ctcgtggcga tggcgctcgg ctcggcggcg tgcgcgccga
    16201 tggggcccgg cggccatcac gccaggacgt ccacggtcac ggtggccggg accggcacgg
    16261 tgtcggccgc gcccgacatg gccgagatca ccaccggggt ggtgacgcag gcgcccactg
    16321 cggcgcaggc gctcgccgcc aacagccagg cgatggagcg gctgctccag gccctcggta
    16381 ccctcggcgt cgccgcgcgc gacatccaga ccacgaacat cagcgtgtcg ccgctccgcc
    16441 gccagggccg ggacgggcag ccgcccgaga tcaccggcta cgaggtgacc aaccaggtgc
    16501 gggtgaaggt gcgcgacctc tcgcgcctgg gccgcgtgct cgaccagcag gtggggcagg
    16561 gcgccaatct cgtctacggc atccagttcg ggcggcagga gccggcgccg ctgctggacg
    16621 aagcgcggaa gcgcgccatg gccgacgcgc ggcgcaaggc ggagctctac tccgccgccg
    16681 tcgccctgaa ggtcggccgc gtggtggcgg tgcaggaggc gggcgccgcg tcgccgcgcc
    16741 cggagatggc cccgcgcgtg acgatgtcgg cggcggtgcc cgtcgcgccg ggcgagcagg
    16801 agattcaggc ctccgtgacc gtgacgttca ccctcgagcc gtgaggcggc catgaccgct
    16861 ccccacttcc tcagcgccaa gcagcttgct tccaacatcc gccgccgcaa gatcggctgt
    16921 ctcgaggcgc tcgacctgtt cctcgcgcgc gtggaccgct tcaacccgga gctgaacgcg
    16981 atcgtggcca gcgacgtggc ggcggcccgc cgccgcgccc gtcaggccga cgccgccctg
    17041 caaaagggga aggtctgggg cccgctgcac ggcgtcccga tgaccgtgaa ggagtcgtac
    17101 gacgtggtcg gcatgccgac cacgtggggg ctcagcgagc tgaagaacaa catgcccccg
    17161 cgcaacgcgc tggcggtgga tcgcctgctc ggggcgggcg tggtgctctt cggcaagacc
    17221 aatgtcccgt cgtggctggc cgactggcaa tccttcaatc cggtctacgg caccaccaac
    17281 aacccctggg atctctcccg gacgccgggc ggatcctcgg gcggctcggc agccgcgctc
    17341 gccgccggcc tcaccggttt cgaggcgggc agcgatatcg gcgcgtccat ccgcaatccc
    17401 gcgcactact gcggggtcta cggccacaag cccacctacg gcatcgtgcc gccccgcggc
    17461 caggccctgc ccggccgggt ggcgcagggc gacatctcgg tggtcggccc gatgggtcgg
    17521 agcgcggacg acctggcgct cgggctctcg gtgatggcgg gcgccgacga gatcgaccgc
    17581 gcgggctgga ggctcgcgct gcccgccccg cgccggaagg cgctgcgcga gttcaaggtg
    17641 gcggtgatgc ggaccgatcc ggtctccgag gtggacgacg aggtccaggc gcgcgtgcag
    17701 gccgtcgccg atttcctcgc ggggaagaag gccaaggtga gcgacaccgc gcggcccgcc
    17761 atcgacaccg cggaggccca gcgcgtctac gtctttctcc tgcgcgcggc gaccgcgggc
    17821 cggcagacgt cagagcagtt cggtcggaac gcggagatcg cgcgcgggct ccggccggac
    17881 gacgagagct actacgcgcg gatgacgcgg gcgaacgtcg ccccgcaccg cgactggctg
    17941 gccgccaacg aggcgcggca tcagatgcgc tggcgctggg ccgagttctt ccgcgactac
    18001 gatctgctgc tgtgcccggc cgcggcgagc ccggctttcc cccacgacca cgcgggcgag
    18061 cgccacgagc ggaccatcgc cgtcaacggc cgccgcgtgc ccaccaccga ccagctcttc
    18121 tgggccggct atccaggggt ggccttcctg ccctccacgg tggcccccgc gggattctcg
    18181 cgcgccgggt tgccgatcgg cgtgcagatc gtcggcccgc agtacggtga ccgcacctgc
    18241 atcgagctgg cccgcctgct cgagcgtgag ttccagggtt tcgttccccc gccgggctac
    18301 tgaggagaac ccattggcga agaaacgcgt ggtcgtgacg ggggcggcgg gctacgtcgc
    18361 ccagcggatg ctgccggagc tccgggagcg ctgggacctg gtcctgctcg acgtgcgccc
    18421 caccacccgc gacggcaaga cggtgcccgg cctgatggtg gccgacctga cgaagccgga
    18481 ccgcaacgag taccgccagc acttccgggg ggcggacgcg gtgattcact gcggcttcgt
    18541 gagcgcgccc cggctcgacg ccaccacctg gcaggacaac agcgacgcca agttctgggc
    18601 cgagcacacc aacgtggcgc tcgcctacaa cgtctgccgg gtcgccctcg aggagggcgt
    18661 gcggcgggtg gtggtgtgca gctcgaacca cgcggccgac tactacgagc gcctggtgtg
    18721 ggacggccgg ctcgacatgg tgactcccga gatgccgccg cgctcggaca actggtatgg
    18781 ctgggccaag gcggcgtacg agctgctggg cttcgtcttc gccaccggga aggtggacgg
    18841 gcgcaagctc gagatcgtgc agtggcgcat cggggggccg cgcgacgacg atatcgacca
    18901 ggtgaagccg ggcgacatca aggtcatgca ccgcgcgctg ggcgcctatc tctcgaagcg
    18961 ggaccaggtg cagcaggcgg tgcgcatggt ggagaccgag gacatccggg acgagcacgg
    19021 ggtgccgttc ctcatcgtct acggcatcag cggcaacacc caccgcttct ggtccatcgc
    19081 gagcgcgcgc gtgaagctcg gctacgcgcc ggaggacgac agccaggtga acttcgccga
    19141 caagatcgcg gccatcgcgc gcgcggcccg gaggtgatca tgaacgcgga agaggagctg
    19201 aaggcgctcg aggggacgct ggtcacgttc cgggagacgc tcaaggaagt gaaccggctg
    19261 agcggcgacg gcatggtcgc ggtacgggag gaatggctcc tgcgcatcaa ggagctcgag
    19321 ctgcagcgcg agcacctgag ccacgtcgtg cgcaagaacc gccgccgcgt cggctagtct
    19381 tctccctctc cccggagggg agagggtagg gtgagggggg atcgagtggc gcgggagctc
    19441 cggttcgtcc aggtggatgt gttcacccag cgggtgtcgg gcgttcccga ggatccggct
    19501 accggctcgg cgagcgggcc gctcggcgcc tacctcgtgg agcgcgggct ggtcgcgccg
    19561 gccgacaccg tcgacatcgt gagcgagcag ggcacgcgca tgggccgacc gggcttcatc
    19621 cggatccgcg tcgacatgcg cgcggggcgc gtcagcgaga tcctggtggg gggtcgcgtg
    19681 gtgcccgtga tcgagagccg gctgcgggtg ccctgacgcc atgtacatcg ccaagcagtt
    19741 ccagatcacc gacaccgcat ggtgccacac gctgatgcgc gtccagagct tcgcgaccat
    19801 ggtcacgacc gacgatacgg gcgtgccctt cgcgacccac ctgccgatcc tggtggatcc
    19861 cgcgcgcagc ccgctcggca ccctgcgcgg ccacgtggcc cgcgccaacc cgcactggcg
    19921 ctacctgtcc gccggccggc cgaccctcgt ggtcttctgc ggtgcccacg cctacgtctc
    19981 gccctcctgg tacgccacgc atccgagcgt gcccacctgg aactacgtgg cggtgcacgc
    20041 caccggcacc ggcacgctgg tcgaggaccc cgagcaggtg aggacgctgc tcaccgacct
    20101 ggtgcggacc tacgagagcg gcgggccgac cgcgtggtcc ctcgaggggc tgtccgcgga
    20161 ttacctggcg ggcatgcagc ggggcatcgt ggccttcgag atccctatcg agcggctcga
    20221 gggcaaggcc aagctcagcc agaaccgtga cgcggtggac caggcgcgca cccgcgaggc
    20281 gctggccgcc tcggacgacc cgctggcgcg ggcggtggcg gccctgatgg ccggcgcgcc
    20341 gccgcgttga ccgctactcc ccgcggaaga ccggcttccg cttctcgagc atcgcgcggc
    20401 gcccttcctg cgcgtccgcg ctcgtgcgcg cccgggtggc ggccgcgtcc aggtcgtcgt
    20461 gggcgatccg ctcccgcgtc gaggtcgcgc gcaggatgct cgccttgatt ccggccagcg
    20521 agagcggcgc gttgtccgcg atgcgacgcg cgagcgcctg caccgcggcc gacaggtcgt
    20581 ccgcgggcac cacctggtgc accatgccga tctcgtaggc gcgccgcgcg tcgatcggct
    20641 ggcccgtgaa caggagctga cgcgtttgcg cggggccgat gatctcgacc agcttctggc
    20701 cgagcgggaa ggggatcacg agcccgatgc gcgccagcgg catgccgagc cgcgcgggct
    20761 ccgcggccac ccgcaggtcg cagtgcagcg ccagctcgca gccgcccgcg atggcgtcgc
    20821 cgtggagcgc ggcgatcgtg ggatgcttcg atcgctccac ccgccgcagc acgtccacca
    20881 cgttgccttc cggatcggcc tcgccgcggc gctgctgcat ctccttgaga tccatgcccg
    20941 cgcagaaggc gggcccggcg ccccgcacca cgaccacgcg cacgtcgcgg cggccgtcca
    21001 ggtcgtcgaa ggtcgcgcga agcgcctgca ggagcggcgt gttcatggca ttgcgctggt
    21061 ccggtcggtt catcgtgacc gtcgcgatgc ccgagtcgac cgagagaaga atttcatccg
    21121 ccatgtgtcg cctccggatt atgggcgcgg cgccatcacg cgcagggcat tcagcatcag
    21181 gtcacgcttc ttcttcggat cctgctcggt ggtcatgaac gagaggaacg cggtgtcgat
    21241 accgcggtcc aggtagcgct ggatgcgggc cttgagctcg gccaggctcc cgcgcgggat
    21301 caggtcgtcc atgacccggg tggggatggc ggccaccgca cccttgcgat cgccggcgcc
    21361 ccacgcggcc cacatgggcc cgagcgcctc ggtgcggccg agccactcct ggaacgctcg
    21421 gtagaccggg acgttcagat acccggcgat gtggcggcgc accaccaggt ccgattcggg
    21481 gccgggcgga tcgaggttga cgaagagccg cgcggtgacc tcgatggcct cgggatcgcg
    21541 cccggcccgc ttggcggcct cgcgcaccac cgcgacggag cgcggcacgt cgtcgggcga
    21601 gagccagttg aggatcacgc cgtcgcccac ctggcccgcg acccgcagca tcccttcgcg
    21661 tagggcggcg atgtggatcg gtactggaag cgcaggcacc ttggt
//
LOCUS       DASUFA010000013         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_3363, whole genome shotgun sequence.
ACCESSION   DASUFA010000013 DASUFA010000000
VERSION     DASUFA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3363"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VFX87_00555"
     CDS             complement(<1..1364)
                     /locus_tag="VFX87_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4993418.1"
                     /translation="MIVLGINETHCATAAVLRDGRVIGCASEERFSRLKNDAGYPRRA
                     IDALLHELAITPQQIDVVALAGARAASREWLNRVLHDEDYVKEYYGVSWPSRRRTFGK
                     KVRKWGAKFGLIDASRGKFGISQRERLGFVTEHLSLAADRIVCLDHHTCHAAAAYWGS
                     GFAGRDALVLTNDNSGDGLCATASTGRGVTLERHEATPSAPGSVGAFYSFVTLAMGMK
                     FGEHEYKVMGMAPYAPEKYTARAEAALREVFDLEEGRPARFRWRAQGERYQLLLRAMV
                     GLRFDWVAGGAQRLLEDVLLRWSRLMRQRYGGGRLALGGGVFMNVKANMLLGQEDWVE
                     ELFAFPSCGDESNAVGAAYLGYVQECARRGLAATPQPFGAAYLGPSVTDEEAEAVIRE
                     RRLEGQYKVAFHDRIEERIAELLVSDGVVARCAGRMEFGARALGNRSILANPSDHRVV
                     GLINR"
     gene            complement(1439..2776)
                     /locus_tag="VFX87_00560"
     CDS             complement(1439..2776)
                     /locus_tag="VFX87_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4993419.1"
                     /translation="MGATELWELSAAEAAALIRAGRLSSVELVESCLGRIRETEPRVE
                     AWIYLDAEYAVAQAKASDEWRLSGRPIGGLQGVPVGLKDIIDTGDMPTENGSVLHAGR
                     TPARDAAVVERLRAAGAVIMGKTVTTEFATRTPGKTRNPHNPRHTPGGSSSGSAAAVA
                     AGMVPLSLGSQTGGSTIRPGSYCGVYAFKPTHGLVSRHGMFKLSRSLDHVGLFARTIE
                     DLGLMLEEVAGYDERDPDTHPRGRGPYRAAAMEEPPIEPRFGFFRSGRWDRIAEDARE
                     ALEEVVAHLGGLVEEFELSAGADELAEWHRVIMDSEVAVNLRREWETGRDRLSAPLRA
                     RIEAGHAVRAPEYLAARAAAGPLLATFTELFEQRYDAIVTPAVDGTAPAGLESTGEPT
                     FCTLWTMLGLPSVSLPLLQGANGLPLGVQLVGQRHGDGRLLRTARWLAESVGA"
     gene            complement(2910..>3315)
                     /locus_tag="VFX87_00565"
     CDS             complement(2910..>3315)
                     /locus_tag="VFX87_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX4993420.1"
                     /translation="GGQSLIERWVLSMNLSPLGFLFVAQLLIFLLGWPLEWTEIIVIF
                     CPIFIPLLSHFDVDPILFGTMVAVNLQAAFLSPPVAMSAFYLKGVAPKHVTLNQIFAG
                     MMPYMVIVLVCLAFMYIWPGMTLWLPNFLYGN"
BASE COUNT          543 a         1247 c         1073 g          452 t
ORIGIN      
        1 cggttgatca gcccgaccac ccggtggtcc gagggattcg cgaggatcga gcgattgccg
       61 agcgcgcgcg ccccgaactc catccgcccc gcgcagcggg ccaccactcc atccgagacc
      121 agaagctccg cgatgcgctc ctcgatgcgg tcgtggaagg ccaccttgta ctgcccctcg
      181 agacgccgct cgcggatgac tgcctccgcc tcctcgtcgg tcaccgaggg cccgaggtac
      241 gccgccccga acggctgcgg cgtggcggcc aggccgcgac gcgcgcactc ctgcacgtac
      301 ccgagatagg ccgcgcccac cgcgttcgat tcgtccccgc aggacgggaa ggcgaagagc
      361 tcctccaccc agtcttcctg cccgaggagc atgttggcct tcacgttcat gaagaccccg
      421 ccgcccaggg cgagccgccc gccgccgtag cgctgccgca tgaggcggct ccagcgcagc
      481 agcacgtcct cgagcaggcg ctgggcgccc cccgcgaccc agtcgaaccg taggcccacc
      541 atggcgcgca gcaggagctg gtagcgttcg ccctgagcgc gccagcggaa gcgggccggc
      601 cggccttcct cgaggtcgaa gacctcgcgc agggccgcct ccgcccgcgc cgtgtacttc
      661 tccggcgcgt acggcgccat gcccatcacc ttgtattcgt gctcgccgaa cttcatcccc
      721 atcgccagcg tgacgaagga gtagaaggcg cccaccgagc ccggggcgct cggcgtggcc
      781 tcgtgccgct cgagggtcac gccccggccg gtggaggcgg tcgcgcagag gccgtccccc
      841 gagttgtcgt tggtgaggac gagtgcgtcg cgtccggcga agcccgagcc ccagtaggcg
      901 gccgccgcgt ggcaggtgtg gtggtcgagg cacacgatgc ggtcagccgc gagactcagg
      961 tgctcggtga cgaagccgag ccgctcacgc tgggagattc cgaacttgcc gcgggaggcg
     1021 tcgatcagcc cgaacttggc gccccacttc cgcaccttct tcccgaaggt acgacgccgt
     1081 gagggccacg agaccccgta gtactccttg acgtagtcct cgtcgtgcag cacccggttc
     1141 agccattccc gcgaggccgc gcgggcgccc gctagggcca ccacgtcgat ctgctggggg
     1201 gtgatcgcca gctcgtgcag gagggcgtcg atcgcgcggc gcgggtagcc ggcgtcgttc
     1261 ttgagccggc tgaagcgctc ctcggaggcg cagccgatga cgcgcccgtc ccggaggacg
     1321 gcggcggtcg cgcagtgggt ctcgttgatg ccgaggacga tcatgtcccc ggtagtatac
     1381 cgcccggcgc ccgcgcgacc ctctcccctg aggggagagg gcagggtgag gggtgggtct
     1441 aggcccctac ggactccgcg agccagcgcg cggtgcgcag gagccggccg tcgccgtggc
     1501 gctggcccac gagctgcacg cccagcggca gaccgttggc gccctggagc aggggcaggc
     1561 tcacggaggg caggcccagc atggtccaca gcgtgcagaa ggtcggctct ccggtggact
     1621 cgagccccgc cggcgccgtc ccatctacgg ccggcgtcac gatcgcgtcg tagcgctgct
     1681 cgaacagctc ggtgaacgtg gcaaggagcg ggcccgccgc cgcccgcgcg gcgaggtatt
     1741 cgggcgcccg caccgcgtgg ccggcctcga tgcgcgcccg tagcggcgcc gacagccggt
     1801 cccgccccgt ctcccactct cgacgcaggt tgaccgccac ctccgaatcc atgatcacgc
     1861 ggtgccactc ggccagctcg tcggcgcccg cgctcagctc gaactcctcc accagcccgc
     1921 ccagatgcgc gaccacctct tcgagcgcct cccgcgcgtc ctcggcgatg cgatcccacc
     1981 gcccggaccg gaagaacccg aagcgcggct cgatcggcgg ctcctccatc gccgccgccc
     2041 ggtacggtcc ccgcccccgc ggatgcgtgt cgggatcgcg ctcgtcgtag ccggcgacct
     2101 cctccagcat cagccccaga tcctcgatcg tccgcgcgaa gagcccgacg tgatcgaggc
     2161 tgcgcgagag cttgaacatc ccgtgccgcg acaccaggcc gtgagtcggc ttgaaagcat
     2221 agaccccgca gtaagacccg ggccgaatgg tcgacccgcc ggtctggctc ccgagcgaca
     2281 gcggcaccat ccccgccgcc accgccgccg ccgacccgct agacgaccca ccgggcgtgt
     2341 gcctagggtt gtgcgggttc cgcgtcttgc cgggggtccg cgtggcgaac tcggtggtga
     2401 cggtcttccc catgatcact gcgccggccg cccgcaaccg ctccaccacc gccgcgtccc
     2461 gcgccggcgt ccgcccggca tgcagcaccg acccgttctc ggtcggcata tcccccgtat
     2521 cgatgatgtc cttcaacccc acgggcaccc cctgtaaccc cccgatgggt cgcccggata
     2581 atctccattc atccgaagcc ttggcctgcg ccaccgcata ctccgcatcc agatagatcc
     2641 aggcctccac ccgaggctcc gtctcccgga tccgccccag gcagctctcc accagctcca
     2701 cggagctcaa ccgccccgcc cggatcaacg ccgccgcctc cgccgccgac agctcccaga
     2761 gctccgtagc tcccatagcg ccggagtata cggtcaaccc cagggaagga aaatcacaga
     2821 agccaatcca agaggaagct gccaagaacg ccgagcagaa gcccacgcga ggagaggccg
     2881 gtgggggccg ggccagcacg caccgacgac tagttgccgt acaggaagtt cggcagccag
     2941 agagtcatgc ccggccagat atacatgaac gccaggcaca cgagcacgat taccatgtac
     3001 ggcatcatcc ccgcgaagat ctggttgagc gtcacgtgct tcggcgccac gcccttcaga
     3061 tagaacgccg acatcgccac cggcggcgac aggaacgcgg cctgcaggtt caccgccacc
     3121 atcgtcccga acaggatcgg gtcaacgtcg aagtggctca ggagcgggat gaagatcggg
     3181 cagaagatca cgatgatctc ggtccattcg agcggccagc cgagcaggaa gatcaacagt
     3241 tgggcgacga acaggaaacc gagcggcgag agattcatgc tgagcaccca tcgctcgatc
     3301 agcgactggc cgccg
//
LOCUS       DASUFA010000014         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_461584, whole genome shotgun sequence.
ACCESSION   DASUFA010000014 DASUFA010000000
VERSION     DASUFA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461584"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFX87_00570"
     CDS             <1..408
                     /locus_tag="VFX87_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX4993421.1"
                     /translation="TRGASHRRLAVSLGATWVGDDTHRPPVTLDRAITFAPSGDVVVA
                     ALASLRKGGVVAINAIHLDRIPEFDYDRLLWGERQIRSVTNMTRADAREFLQVASAIG
                     LRPSVTAFPLDRVNEALRAIKGDAIDGAAVIVP"
     gene            481..1521
                     /locus_tag="VFX87_00575"
     CDS             481..1521
                     /locus_tag="VFX87_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX4993422.1"
                     /translation="MRESRSSGLVSGIGAGALAALLLGVAARGEADLRLAREGYPVVD
                     GSTSTQPLGMLVAGRVTRTSVEWRRTSFFDPTRLLALTAAPYDDSRKLSLEPTAAEPR
                     GADLGTALSSIGQLAERVRHSGTHQSYARLAAGQAELVLTAREPTPAEREAVRRGGTE
                     IVVLPIARDAFVFLRHASNPVTGVTLDQVRDIYAGVLTSWKILGGADRPIVAYQRDAT
                     SGSQVEMAELVMRGRPMREGPDIRVTMSMFGPFNAIRHDPTGIGYSYHYYERFMAAVP
                     EVTTLAIDGIPPERATIASGRYPLGTAVYLAHREDLPPGGAAARLRDWLRTPAGQAVI
                     AESGYVPLRPAP"
     gene            1518..>2909
                     /locus_tag="VFX87_00580"
     CDS             1518..>2909
                     /locus_tag="VFX87_00580"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX4993423.1"
                     /translation="MKVELPYGDGAIAATLPAGTRRLSSAPEGALPPLADLAGAVRAA
                     LAAPRGLPRIRDLVKPGARVTIAFDDHTVGSFGPIRPVAIRAVLDELAEAGVTRSQVR
                     LVCANALHRMLRPGELRRLLDDRLADEFAGRLSCHDAEDPAQIADLGPTPEHGYPVEV
                     SRLVTDADLTVYVNTNYIRGFTGGWKSVCIGLSTWKSIRVTHDPDGMSMSLERNRMHA
                     VLDEMGHHLERRLGRRIFKIDTLLADPFQAAQVLAGGVDETRRAALETQARRFPSRRA
                     AAPDPCDILVYGVPDSSPYAIWASVNPILTLISMGLGYLGGMIDAAGKPGCTVVMAAA
                     VRDAWDRVHHPSYPDVWERVLPETRDAYEITARFEAVYARHAEYIEAYRHRFAFHPIH
                     GILATHPLKRLRKVGRVIVAGARDAHVPRHLGFDVASTVEEAIDRALRLHGPGASIGV
                     VEQAGMPADPERRP"
BASE COUNT          378 a         1066 c         1038 g          427 t
ORIGIN      
        1 acccggggtg cgtcgcaccg ccggctggcg gtctcgctcg gggccacctg ggtcggcgat
       61 gacacgcacc gcccgcccgt cacgctcgat cgtgcgatca cgttcgcgcc cagcggtgac
      121 gtcgtggtcg cggcgctcgc gagcctgcga aaggggggcg tggtggcgat caatgccatt
      181 catctggatc gcatccccga gttcgactac gaccgtctcc tgtggggcga gcggcagatt
      241 cggagcgtga cgaacatgac tcgagccgat gcgcgggagt tccttcaggt cgcgagcgcg
      301 atcggcctcc ggccctccgt gaccgccttc ccgctcgatc gggtgaacga agcgctccgc
      361 gcgatcaagg gcgatgccat cgatggcgcg gccgtcatcg ttccgtagag gggttcatcg
      421 ggccgggtga tacactctcg ccggcgtacg cccgtgtcgc gatggtggcg gaggtgcgaa
      481 gtgcgtgaat cgcgctcgtc cggattggtg agcgggatcg gggcgggggc cctggccgcc
      541 ctcctgctcg gcgtggccgc gcggggcgaa gccgacctcc ggctggctcg ggaggggtac
      601 cccgtggtgg acgggtccac ctccacccag ccgctcggga tgctggtggc cggacgggtg
      661 acccggacga gcgtcgagtg gcgccgcacc tcgttcttcg accccacgcg gctcctcgct
      721 ctcacggccg ccccgtacga cgactcgagg aagctctcgc tcgagcccac ggcggccgag
      781 ccacgcgggg cggatctcgg gaccgcgctt tcctccatcg ggcagctcgc ggagcgcgtc
      841 cggcactcgg gcacccatca gagctatgcg cgcctggccg ccggccaggc cgagctcgtg
      901 ctgaccgccc gcgaacccac gccggccgag cgcgaggcgg tccgccgcgg cggcaccgag
      961 atcgtcgtct tgccgatcgc gcgggacgcg ttcgtcttcc tgcgccacgc gagcaatccc
     1021 gtgacgggcg tcaccctgga ccaggtgcgc gacatctatg ccggcgtcct gaccagctgg
     1081 aagatcctcg gtggggccga tcgccccatc gtggcctatc agcgcgacgc gacatcggga
     1141 agccaggtgg agatggcgga gctcgtgatg cggggtcggc ccatgcggga gggcccggac
     1201 atccgggtca ccatgtccat gttcggtccc ttcaacgcca tccgccacga cccgaccggg
     1261 atcgggtact cctaccacta ctacgagcgc ttcatggcgg cggtgcccga ggtgaccacg
     1321 ctggccatcg acgggatccc gcccgagcgg gcgacgatcg cctccggccg ctatcccctg
     1381 gggacggcag tctatctcgc ccatcgcgag gacttgccgc ccggcggtgc ggcggctcgc
     1441 ctgcgcgact ggctgcgcac gccggccggc caggcggtga tcgcggagag cgggtacgtg
     1501 cccctgcggc cggcgccatg aaggtcgagc tgccgtacgg ggatggagcc atcgccgcca
     1561 ccctccccgc cggcacgcgc cggctctcca gcgcgccgga gggcgcgctc ccgcccctcg
     1621 ccgatctcgc cggcgcggtg cgcgccgcgc tggcggcgcc gcgcggcctg ccccgcatcc
     1681 gcgacctcgt gaagcccgga gcccgggtca ccatcgcctt cgacgatcac acggtcggct
     1741 cgttcggtcc catccggccc gtcgcgatcc gggcggtgct ggacgagctg gccgaggcgg
     1801 gggtgacgcg ctcgcaggtg cggctggtct gcgcgaacgc gctccaccgg atgctgcggc
     1861 cgggcgagct gcggcggctc ctcgacgacc gcctcgccga cgagttcgcc gggcgcctgt
     1921 cctgccacga cgcggaggat cccgcccaga tcgccgatct cgggccgacg cccgagcacg
     1981 ggtatcccgt ggaggtcagc cggctcgtca ccgacgccga cctcaccgtg tacgtcaaca
     2041 ccaactacat ccgcggcttc accggcggct ggaagtccgt gtgcatcggg ctgtcgacgt
     2101 ggaagtcgat ccgcgtcacc catgaccccg acggcatgtc gatgtcgctg gagcgcaacc
     2161 gcatgcacgc cgtcctcgac gagatgggcc atcacctcga gcggcggctc ggccggcgca
     2221 tcttcaagat cgacacgctc ctcgccgacc cgttccaggc cgcgcaggtg ctggcgggcg
     2281 gcgtggacga gacccggcgg gccgcgctcg agacccaggc gcggcgcttc ccctcgcggc
     2341 gcgccgccgc ccccgacccg tgcgacatcc tcgtctacgg ggtgcccgat tcgagcccgt
     2401 acgcgatctg ggcctcggtg aatcccatcc tgacgctgat ctcgatgggg ctgggctatc
     2461 tcggcggcat gatcgacgcc gccgggaagc cggggtgcac cgtggtgatg gcggcggcgg
     2521 tacgggacgc gtgggaccgg gtgcaccatc cctcgtatcc ggacgtgtgg gagcgcgtgc
     2581 tgcccgagac tcgggatgcc tacgagatca ccgcccgctt cgaggccgtc tacgcgcgcc
     2641 acgcggaata catcgaggcc taccgccatc gcttcgcctt ccatcccatt cacgggatcc
     2701 tcgccacgca tccgctcaag cgcctgcgga aggtgggccg cgtgatcgtg gcgggcgcgc
     2761 gtgacgcgca cgtgccccgt cacctgggct tcgacgtggc gtcgacggtg gaggaggcca
     2821 tcgaccgcgc gctccggctc catgggcccg gcgcctcgat cggcgtggtg gagcaggcgg
     2881 gcatgcccgc ggatccggag cgccggccg
//
LOCUS       DASUFA010000015         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1605309, whole genome shotgun sequence.
ACCESSION   DASUFA010000015 DASUFA010000000
VERSION     DASUFA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605309"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VFX87_00585"
     CDS             <1..1298
                     /locus_tag="VFX87_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX4993424.1"
                     /translation="QHYLEYVQQLLESAYGADVVFKGGLQVYTTLSPTMQLKAEQSLR
                     DGLKALETRRLKASEKAGTAPVTERPEGALLALDPQTGHIKAMVGGYDFFKSEFNRAT
                     QARRQPGSSFKPFVYIAALEAGLTPATVVEDSPVEFPGGPNGKPWKPENYDRKFRGPL
                     TLQQALEESINVAAVKVQERIGLRRTIDVARRLGVESPLGENLSLALGTSELSLLELT
                     SAYGTLANQGQWLHPTAVRYVLDAQGKLLEENVPEPTQVLTPELAFVATQMLKGTIER
                     GTGVAARALGRPAAAKTGTTNDYSNAWFIGFTPQLATGVWVGYDRPRSLGKDETGSRV
                     AVPIWTTFMQAALAGLPAEDFPIPEKVVLVPVDLDRAGTCSRPALMAFVVGTEPSQVC
                     GPARFASGVRPPSAALPAAPAAVVPAGAPGAPREGGQTP"
     gene            complement(1295..2809)
                     /locus_tag="VFX87_00590"
     CDS             complement(1295..2809)
                     /locus_tag="VFX87_00590"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX4993425.1"
                     /translation="MPVTMDALVSLSKRRGFVFQSSEIYGGTGSCWDYGPLGVELKQN
                     IKRLWWRDFVQSRGDMVGLDASILMHPMVWTASGHVDKFTDPMVDCHECRHRFRADHV
                     DALPWTHYCPATKGNKFVIPAGEACRHCGVGRTLCPECGKGELTAPRQFNLMFKTFMG
                     PVEEEASLTYLRPETAQAMFVNFDNVLQSMRLKLPFGIAQAGRSFRNEITPGNFIFRT
                     REFEQMEIEFFVNPHDTVEGRPADEWWHDRWIADCMAWFRRYGLREENLRLREHEKAE
                     LAHYAKRTVDIDYQFPIGWSELMGIANRSDYDLKQHSKFSGKSLTYFDEERKEHIVPF
                     VVEPAAGVDRALLAFLCDAYREEEVRGEKRVVLRFHPELAPVKVAVLPLLKKREEIVR
                     TAHGIRTALSRHFRAVYDDTAAIGRLYRRQDEVGTPYCVTVDVQTVGDRDKGETGDGR
                     VTIRDRDSMQQVRVPEGELLPVFQGLLEGGDWGAVVARFGGVTADAARPGEGDR"
     gene            complement(2823..3617)
                     /gene="recO"
                     /locus_tag="VFX87_00595"
     CDS             complement(2823..3617)
                     /gene="recO"
                     /locus_tag="VFX87_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX4993426.1"
                     /translation="MALSRTRAVVVSRRAMGEQDRLVEFYTRDHGKVRGVAKSARRPR
                     SRFVAALEPFTLGELVFFDTGRSGLVRVDHFDIVHSFVRVRENLDRLGRGAWMCESLT
                     RLSADRDPHPALFGLLVRSLRALETVVTPERVAICFALRAVDLLGHRPRLDRCVACGR
                     PFPFAGASLDPEAGGLVCGACAAGRPAVPLSGPAVGMLARLRGLRWEEALAAGLGAGL
                     EDELGTALDGVVTRLIGGLPRSLRFIGQTRRGLAGVAEPPPARRRT"
     gene            complement(3620..>4423)
                     /gene="era"
                     /locus_tag="VFX87_00600"
     CDS             complement(3620..>4423)
                     /gene="era"
                     /locus_tag="VFX87_00600"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX4993427.1"
                     /translation="VSRRPQTTRTRITGIRHLAHAQVVFVDTPGLHAGQGRLGELMQR
                     AAERALEDVDLVCFVAEATERADRLDAAALSRLEGVRAPVICCLNKVDLVAPKSRLLP
                     LIAAYRARYPFQDIVPLSAQTGAGCDALMNLVLAALPERPPYFPRESLTDQPETFFVA
                     EAIREKIFRLTNQEIPYACAVRVEELTERKRPECLYIKATIFVEQDSQKGILIGKQGA
                     MLKRIGSTTREDLERFFGIKTYLDLRVAVRKNWRKDEGALREFGFLLTS"
BASE COUNT          676 a         1601 c         1532 g          614 t
ORIGIN      
        1 gacagcacta cctcgagtac gtccagcagc tcctggagtc cgcgtacggc gccgacgtgg
       61 tcttcaaggg cgggctgcag gtctacacca cgctgtcgcc gacgatgcag ctcaaggccg
      121 agcagtcact gcgcgacggg ctcaaggcgc tggagacccg gcggctcaag gcctccgaga
      181 aggccggcac cgccccggtc acggagcggc cggagggcgc gctgctcgcc ctcgatcccc
      241 agaccggcca catcaaggcc atggtcggtg gctacgactt cttcaagagc gagttcaacc
      301 gcgccaccca ggcgcgccgt cagccgggct cctccttcaa gcccttcgtc tatatcgcgg
      361 cgctggaggc gggcctcacc cccgccaccg tggtggagga ctcgccggtg gagttcccgg
      421 gcgggccgaa cggcaagccg tggaagccgg agaactacga ccggaaattc cgcggcccgc
      481 tcacgctgca acaggctctc gaggaatcca tcaacgtggc ggcggtgaag gtccaggagc
      541 gcatcggtct ccgccgcacc atcgatgtgg cgcgacgcct gggcgtggag agcccgctcg
      601 gcgagaacct cagcctggcc ctgggcacct ccgagctgtc cctgctcgag ctcacctccg
      661 cctatggcac cctcgccaac cagggccagt ggctccatcc caccgccgtg cgctacgtgc
      721 tcgacgccca gggcaagctg ctggaggaaa acgtcccgga gcccacgcag gtgctcaccc
      781 ccgagctcgc cttcgtggcc acgcagatgc tcaagggcac catcgagcgc gggacgggcg
      841 tggcggccag ggcgctcggc cgccccgccg ccgcgaagac cggcaccacc aacgactact
      901 cgaatgcgtg gttcatcgga ttcacgccgc agctcgccac cggcgtgtgg gtcggctatg
      961 accgcccgcg cagcctcggg aaggacgaga cgggctcgcg cgtggcggtg cccatctgga
     1021 cgactttcat gcaggcggcg ctggcggggt tgccggcgga ggacttcccc atccccgaga
     1081 aggtggtgct ggtgccggtg gatctggatc gggccggcac ctgcagccgg cccgcgctga
     1141 tggccttcgt cgtcggcacc gagccgtcgc aggtttgcgg gcccgcgcgc ttcgcctccg
     1201 gggtgcggcc gccctccgcc gcgctccccg ccgcgcccgc ggcggtggta cccgccggcg
     1261 cgcccggggc gccgcgggag ggcgggcaaa ccccctagcg gtcgccctcg cccgggcgcg
     1321 cggcgtccgc cgtcacgccg ccgaagcgcg cgaccaccgc gccccagtcc ccaccctcga
     1381 gcaatccctg gaacacgggc aagagctcgc cctcgggcac gcggacctgc tgcatgctgt
     1441 cgcgatcgcg gatggtcacg cgaccgtccc ccgtctcgcc cttgtcgcga tctcccaccg
     1501 tctgcacgtc gacggtgacg cagtagggcg tgcccacctc gtcctgccgg cgatagagcc
     1561 gcccgatggc ggcggtgtcg tcgtagacgg cgcggaagtg gcgcgacagg gcggtgcgga
     1621 taccatgcgc ggtgcgcacg atctcctcgc gcttcttgag cagcggtagc accgccacct
     1681 tcaccggcgc cagctcggga tggaaccgga gcaccacccg cttctcgccg cgcacttctt
     1741 cctcgcggta ggcgtcgcac aggaaggcga ggagcgcgcg gtccacgccc gccgccggct
     1801 ccaccacgaa agggacgatg tgctccttgc gctcctcgtc gaagtaggtg agcgacttgc
     1861 cgctgaactt ggagtgctgc ttgaggtcgt agtcgctgcg gttggcgatg cccatgagct
     1921 cgctccagcc gatggggaac tggtagtcga tgtccaccgt gcgcttggcg tagtgcgcca
     1981 gctcggcctt ctcgtgctcg cgcaggcgga ggttctcctc gcgcaggccg tagcgccgga
     2041 accatgccat gcagtcggcg atccagcgat catgccacca ttcgtcggcg gggcggccct
     2101 cgacggtgtc gtggggattg acgaagaact cgatctccat ctgctcgaac tcgcgcgtcc
     2161 ggaagatgaa gttgcccggc gtgatctcgt tgcggaacga ccgccccgcc tgcgcgatgc
     2221 cgaagggcag cttgagccgc atggactgga gcacgttgtc gaagttgacg aacatcgcct
     2281 gggcggtctc gggacggaga taggtgagcg aggcctcctc ctccaccggg cccatgaagg
     2341 tcttgaacat gaggttgaac tggcgcggcg cggtgagctc gcccttgccg cactcggggc
     2401 agagcgtgcg cccgacgccg cagtgccggc acgcctcccc cgccgggatc acgaacttgt
     2461 tgcccttggt ggccggacag tagtgggtcc acggcagcgc gtccacgtga tcggcgcgga
     2521 agcggtgacg gcactcgtgg cagtccacca tgggatcggt gaacttgtcg acgtgcccgc
     2581 tcgccgtcca gaccatcggg tgcatgagga tggaggcgtc gaggcccacc atgtccccgc
     2641 gtgactgcac gaagtcgcgc caccagagcc gcttgatgtt ctgcttgagc tcgacgccga
     2701 gcgggccgta gtcccagcag gagccggtgc ccccgtagat ctcgctggac tggaagacga
     2761 aaccgcggcg tttcgagagc gacaccagcg cgtccatggt gaccggcatg gcgctcagga
     2821 tatcacgtgc gccgccgggc cggcggcggc tcggccacgc cggcaagccc gcgccgcgtc
     2881 tgcccgatga agcggagcga gcgcgggaga ccaccgatga gccgcgtgac gacgccgtcc
     2941 agcgccgtcc ccagctcgtc ctccagcccg gcccccagcc ccgccgcgag ggcctcctcc
     3001 cagcgcagac cgcgcaggcg cgccagcatc cccaccgcgg ggccggacag cggcaccgcg
     3061 gggcggcccg ccgcgcaggc cccgcagacc aagccgcccg cctcgggatc cagcgacgcg
     3121 cccgcgaacg ggaagggccg gccgcacgcc acgcaccggt ccaggcgcgg ccggtgcccg
     3181 agaagatcca ccgcccgcag ggcgaagcag atggccacgc gctccggcgt caccaccgtc
     3241 tccagggcgc gcaggctgcg cacgagcagc ccgaacagcg cgggatgcgg atcgcggtcg
     3301 gcggagagtc gggtgagcga ctcgcacatc caggcgcccc gccccagccg gtcgaggttc
     3361 tcccgcaccc gcacgaagga gtggacgatg tcgaagtggt ccacgcggac cagcccgctg
     3421 cgcccggtat cgaagaaaac gagctcgccc agggtgaacg gctccagcgc ggcgacgaag
     3481 cgggagcggg gccgccgcgc cgacttcgcc acgccccgta ccttgccgtg gtcccgcgtg
     3541 tagaactcga ccagccggtc ctgctcgccc atggcgcggc ggctcaccac cacggcccgc
     3601 gtgcgggaaa gcgccatgct caggacgtga ggaggaagcc gaactcccgc agcgccccct
     3661 cgtctttccg ccagttcttg cgcacggcga cgcggaggtc gagataggtc ttgatcccga
     3721 agaagcgctc gagatcctcc cgcgtcgtgc tgccgatgcg cttgagcatc gccccctgct
     3781 tcccgatgag gatgcccttc tgcgagtcct gctccacgaa gatggtggcc ttgatgtaca
     3841 ggcactccgg ccgcttccgc tccgtgagct cctccacgcg gacggcgcag gcgtagggga
     3901 tctcctggtt ggtgaggcgg aagatcttct cgcggatggc ttccgccacg aagaaggtct
     3961 ccggctggtc ggtgagcgac tcccggggaa aatagggcgg ccgctcggga agggccgcca
     4021 gcaccaggtt catgagcgcg tcacagccgg cacccgtctg cgcggacagc ggcacgatgt
     4081 cctggaaggg gtaccgcgcg cgatacgcgg cgatgagggg cagcaggcga gacttgggcg
     4141 cgaccaggtc caccttgttg aggcagcaga tcacgggggc ccgcaccccc tcgagtcggg
     4201 acagggccgc cgcgtcgagg cggtccgccc gctcggtggc ctcggcgacg aagcagacca
     4261 ggtccacgtc ctccagcgcg cgctccgccg cccgctgcat cagctcgccg agccggccct
     4321 gccccgcgtg aaggcccggc gtgtccacga agaccacctg cgcgtgcgcc aggtgccgga
     4381 tgcccgtgat gcgggtgcgc gtggtctgag ggcgccggga gac
//
LOCUS       DASUFA010000016         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1605523, whole genome shotgun sequence.
ACCESSION   DASUFA010000016 DASUFA010000000
VERSION     DASUFA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605523"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFX87_00605"
     CDS             complement(<1..290)
                     /locus_tag="VFX87_00605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4993428.1"
                     /translation="MPPPRHVIVCGAGVMGAAVAYFLARRGVAVTVVERTGVACAASG
                     KSGGFLALDWCDGSPVGPLARASFALHAELAGELGSDYGYRRMDTYMLAARE"
     gene            371..1210
                     /locus_tag="VFX87_00610"
     CDS             371..1210
                     /locus_tag="VFX87_00610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4993429.1"
                     /translation="MDYGAVMFPTEYSIAPDELARALEERGFESVWFPEHTHIPASRR
                     SPWPGGGELPRDYWSSYDPFIALMAAAGATKRLRLGTGICLVIERDPIVTAKEVATLD
                     RLSGGRVLFGIGGGWNAEEMENHGTAWKSRWRLLRERVLAMKAIWTEREASYQGEFVR
                     FEKIWADPKPLQKPHPPIFIGGDGQRTFDRIVEFGDGWMPIMRPHTSPVEKIPALRER
                     LQKAGRDPKSAPVSIFFAPPRREALDALAAAGVQRAIFGLPAAPRDEVLPKLDAYAAV
                     MRA"
     gene            complement(1186..2190)
                     /locus_tag="VFX87_00615"
     CDS             complement(1186..2190)
                     /locus_tag="VFX87_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX4993430.1"
                     /translation="MKQVLLERYGAPAESVRCVEVPDVGAPAAGEVVFDVLAFPINPA
                     DLSMCRGTYRLRPALPAGIGAECVGRVAAVGAGVTHVKPGDRVINLQRENWAQRRRVK
                     GDDVIPVPAGLDDLQAAMLRINPPTALLLLSDLVALRPGDWIIQNVANSAVGRFVIRL
                     ARARGVRTVNVVRRESLFAELTALGADVCVVDGPDLAEAVRARTGGASIRLGVDAVSG
                     RATARISACVGEGGVVGNYGSMSGEDPIMPRAKLVGDGQTLVGFILGRALATRSLAEI
                     RAIYAELGEQVRQGLLTAPVEKVYAIDDIRTAVAHAERGERAGKILVAPNGQARITAA
                     "
     gene            complement(2187..>2378)
                     /locus_tag="VFX87_00620"
     CDS             complement(2187..>2378)
                     /locus_tag="VFX87_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4993431.1"
                     /translation="LRTLLDHVTRPEFVYRHEWQAGDLVIWDNRCVLHRATPYDTARH
                     QRLMQRTTIAGDPAEPAAR"
BASE COUNT          343 a          862 c          874 g          299 t
ORIGIN      
        1 ttctcccgcg cggccagcat gtaggtgtcc atccggcgat agccgtagtc tgatccgagc
       61 tcgcccgcca gctcggcgtg gagcgcgaag ctcgcgcggg ccagcgggcc caccggggag
      121 ccgtcgcacc agtcgagcgc caggaagccg ccggacttgc cggacgcggc gcaggccacg
      181 cccgtgcgct ccacgacggt gaccgcgacg ccgcgccggg ccaggaagta ggctaccgcg
      241 gcgcccatga caccggcgcc gcagacgatg acgtgccgag gagggggcat ggccggttcg
      301 ccgcacaggg tagtacaatc ccccgcgaac ccccagccca atcgccaacc caatagaagg
      361 agggcctctc atggactacg gcgccgtcat gttccccacc gagtactcga tcgccccgga
      421 cgagctggcg cgcgccctcg aggagcgcgg tttcgaatcg gtgtggtttc ccgagcacac
      481 gcacatcccg gcgagccgcc ggagcccgtg gccgggcggg ggcgagctgc cccgcgacta
      541 ctggtcgtcc tacgatccgt tcatcgcgct gatggccgcg gccggggcca cgaagcgcct
      601 ccggctcggc accgggatct gcctcgtgat cgagcgcgat ccgatcgtca ccgccaagga
      661 ggtggccacg ctcgaccgcc tctcgggcgg ccgggtgctc ttcggcatcg gcggcggctg
      721 gaacgccgag gagatggaga accacggcac cgcgtggaag agccgctggc ggctgctgcg
      781 ggagcgcgtg ctcgcgatga aggcgatctg gacggagcga gaggcgagct accagggcga
      841 gttcgtgcgc ttcgagaaga tctgggccga tcccaagccg ctccagaagc cacacccgcc
      901 gatcttcatc ggcggggatg gccagcgcac cttcgaccgc atagtcgagt tcggcgacgg
      961 ctggatgccg atcatgcgcc cgcacaccag cccggtggag aagatccccg cgttgcggga
     1021 gcggctccag aaggccggcc gcgatccgaa gtcggcgccg gtgtccatct tcttcgcgcc
     1081 gcccaggcgg gaggcgctcg acgccctcgc cgcggccggg gtgcagcgcg ccatcttcgg
     1141 cctgcccgcg gcgccccgcg acgaggtgct gccgaagctc gacgcctacg cggcggtgat
     1201 gcgcgcctga ccgttcgggg cgaccaggat cttgccggcg cgctcgccgc gctcggcgtg
     1261 cgccaccgcg gtccggatgt cgtcgatggc gtagaccttc tcgacggggg cggtgaggag
     1321 cccctgccgg acctgctcgc ccagctccgc gtagatcgcc cggatctcgg cgagcgagcg
     1381 ggtggccagg gcgcgcccca ggatgaagcc gaccagcgtc tgcccgtcgc cgaccagctt
     1441 ggcgcgcggc atgatcgggt cctcgccgct catcgagcca tagttgccga ccacgccgcc
     1501 ctcgccgacg caggccgaga tccgcgcggt ggcccggccc gagaccgcgt cgacgccgag
     1561 gcggatggac gcgccgccgg tgcgggcccg gaccgcctcg gccaggtcgg ggccgtccac
     1621 cacgcagacg tccgcgccga gcgcggtgag ctcggcgaag agcgactcgc gccgcaccac
     1681 gttcacggtc cgcacgccgc gggcccgcgc caggcggatc acgaaccggc cgaccgcgga
     1741 gttggcgacg ttctggatga tccagtcgcc cggccgcagg gcgacgagat cggagagcag
     1801 caggagcgcg gtcggcgggt tgatgcggag catggcggcc tgcagatcgt cgaggcccgc
     1861 gggcaccggg atgacgtcat cgcccttcac gcggcgccgc tgcgcccagt tctcgcgctg
     1921 caggttgatc acgcgatcgc ccggcttcac gtgggtgacg ccggcgccga ccgcggccac
     1981 gcgcccgacg cactcggcgc cgatcccggc cggcagggcc ggccgcaggc gataggttcc
     2041 gcggcacatc gagaggtcgg ccgggttgat cgggaaggcg agcacgtcga agaccacctc
     2101 gcccgccgcg ggtgcgccga cgtccgggac ctccacgcag cggaccgact ccgcgggggc
     2161 cccgtagcgc tccagcagga cctgcttcac cgcgccgcgg gctccgcggg atcccccgcg
     2221 atggtggtcc gctgcatgag gcgctggtgg cgcgcggtgt cgtagggggt agcgcggtgg
     2281 agcacgcaac ggttgtccca gatcaccagg tcgccggcct gccactcgtg ccggtacacg
     2341 aactcggggc gggtgacgtg atcgagcagg gtccgcag
//
LOCUS       DASUFA010000017         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_462212, whole genome shotgun sequence.
ACCESSION   DASUFA010000017 DASUFA010000000
VERSION     DASUFA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462212"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2257)
                     /locus_tag="VFX87_00625"
     CDS             complement(<1..2257)
                     /locus_tag="VFX87_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4993432.1"
                     /translation="MLFTSHQIGTERETALTHWKTRLATIAAERTRLVSSWLAARRAD
                     AEVLAAFPAVRAQLAGQGRDDEALARHLSRVAAAYGYVGIVISDAAGRPAARSASDTG
                     LAAGLLEQAAEHAGRVARSRKPRFELGGEGAGRLLVVTVPVFSDGAPAGLDGDTRPLL
                     GVVTLGAKPEAGLYPLLADDAEPTHTGETLLADLDAEPPTFLSPLRQPISPDAEIPKR
                     SLGALGALARAAAQGHEAVGETIDYRGVPALAAVRRVVPTGWSLVLKVDRDEALAEFQ
                     RSGRLAGAAAAFLLLALAGFLIALHREQQRARLLQDRMHQARAMTNLQGYADKIVASV
                     PAGLLLLSEDLHVLSANRAFLESFRLREEDVLGRDLQQLVRAERLLRGAREVLASGAT
                     QQAGLYELYVYARRDTKPARITMSAIHPADDEPPRLLLIVEDLSEEERLQAARQESEQ
                     RYRDLIQGLDAIVWEADARTLTFSFVSRRAETVLGYPIERWLREPDFWARRIRPDDRD
                     SVMQTYRTALTEGRDHEFEYRAIAADGREVWLRDIVHVVRDADGRPTTLRGLTVDLTE
                     LKRSERALHTSEEQLRQAQKMDAVGKLAGGIAHDFNNLLMVIRGDSDLMLRRLAPGHS
                     LRRNAEGIRDAADQAATLTRQLLAFSRKQVMAPRLVDLNGIVASIHAMLQRLLGETIN
                     LVTVTAPDLGGVKADPGQMEQMILNLCVNARDAMPDGGRLTVRTANVDLDEAAAARLS
                     DARPGPYVLLEV"
     gene            complement(2477..3454)
                     /locus_tag="VFX87_00630"
     CDS             complement(2477..3454)
                     /locus_tag="VFX87_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEX4993433.1"
                     /translation="MTATPGWRSLDLPIRVGISSCLLGEEVRYDGGHQKDAYITGVLA
                     RYFTWVPVCPELEVGMGVPREPIRLVGDAAAPRLMGVTSGTDHTERMHEFARRRVDEL
                     RRRDLSGYILKRASPSCGLERVKVYPSDGPPARTGTGLFARALTAALPLLPVEEEGRL
                     NDARLRDNFITRVFAYRRLAALRESEPRPGAVVEFHTAHKYLLLAHSPAAYARLGRLV
                     AEVARPPRARWLDAYGEGFMEALEARATIKKHVNVLQHIMGFFKKQLAAAEKQELLGV
                     IGDYAAGLVPLVVPITLINHYVARFDVAYIRDQIYLRPHPKELMLRNHV"
     gene            complement(3454..3795)
                     /locus_tag="VFX87_00635"
     CDS             complement(3454..3795)
                     /locus_tag="VFX87_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993434.1"
                     /translation="MKATKAEREFFDPAPLPWRPAPGFPSGVWEQVISGGDDEGVTTR
                     LLRFDPGSGNDRVVTHDFWEEIYIISGTLECGGRPYPAGSVAVRPPGMPHGPFHSAAG
                     CVSFEVRYRAR"
     gene            3817..4842
                     /locus_tag="VFX87_00640"
     CDS             3817..4842
                     /locus_tag="VFX87_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4993435.1"
                     /translation="MPTIDSARLEAKATRIFTAMGAPDGDAAWIAALLVRANLRGHDS
                     HGVIRIPQYWESVKKKEVDPRSPVTVTAETPALARLDGGGGFGQVVGRRAMEMAIAKA
                     KAGGLSAASTSRTSHVGRLADYAEMAAGAGLVGMLWANCVHGLNVAPWGGAARRLGTN
                     PHAVGIPGAGGPAMVLDFATSVVAEGKLRVKRNRKQPAPPGWFVDAQGRPQTDPEIFY
                     GTPPGALLTAGEHKGYGLSLAVEILGGILSGTGPAGPPPGVFANGTLMICLDVERFLP
                     LPEFHRQVASLFGWVKSAPLAQGATEILIPGEPEARLEAERRRQGIPIEDQTWSQIET
                     TAAELGL"
     gene            complement(4855..>5166)
                     /locus_tag="VFX87_00645"
     CDS             complement(4855..>5166)
                     /locus_tag="VFX87_00645"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4993436.1"
                     /translation="IWAHAGMSSGPQAVGALLDRYPTLWVDLAIRNSDVAPGGTLDPG
                     WRAVFLRHPDRFLAGTDTWMTSRWDALPGSVTEVRSYLSRLPRDVAEKIAFRNAERLF
                     P"
BASE COUNT          692 a         1822 c         1968 g          684 t
ORIGIN      
        1 ccacctcgag cagcacgtag gggccgggtc gcgcgtcgct caggcgcgcg gccgccgcct
       61 cgtccaggtc cacgttggcg gtgcgcacgg tgagccggcc gccgtcgggc atggcgtcgc
      121 gcgcgttgac gcagaggttc aggatcatct gctccatctg gccggggtcg gccttcaccc
      181 cgcccaggtc gggcgcggtc accgtgacga ggttgatcgt ctcgccgaga aggcgctgga
      241 gcatggcgtg gatgctggcc acgatgccgt tgagatccac gagccgcggg gccatcacct
      301 gcttgcggct gaaggcgagg agctgccggg tgagggtggc cgcctggtcg gccgcgtcgc
      361 ggatgccctc ggcgttgcgg cgcagggagt ggccgggggc caggcggcgc agcatcaggt
      421 cgctgtcgcc gcggatgacc atgagcaggt tgttgaagtc gtgcgcgatg cccccggcca
      481 gcttgcccac cgcgtccatc ttctgggcct gccgcagctg ctcctcgctc gtgtggagcg
      541 cgcgctccga gcgcttcagc tcggtgaggt ccaccgtgag gccgcgcagg gtggtgggcc
      601 ggccgtccgc gtcgcgcacc acgtggacga tgtcgcgcag ccacacctcc cgaccgtcgg
      661 cggcgatcgc ccggtattcg aactcgtggt cgcggccctc cgtgagcgcg gtgcgatagg
      721 tctgcatcac cgaatcgcga tcgtccgggc ggatgcgccg ggcccagaag tcgggctcgc
      781 gcagccagcg ctcaatcggg tagccgagca cggtctcggc gcggcggctg acgaacgaga
      841 acgtgagcgt gcgcgcgtcg gcctcccaca cgatcgcgtc gagaccctgg atgaggtcgc
      901 ggtagcgctg ctccgactcc tggcgggccg cctgcagccg ctcctcctcg gagaggtcct
      961 cgacgatcag gagcaggcgc ggcggctcgt cgtcggccgg gtggatggcg ctcatcgtga
     1021 tgcgcgcggg cttggtgtcg cgccgcgcgt agacgtagag ctcgtagagg cccgcctgct
     1081 gggtcgcccc gctggcgagc acctcgcggg cgccgcgcag gagccgctcc gcccgcacga
     1141 gctgctggag atcgcgcccc agcacgtcct cctcgcggag gcggaacgac tcgaggaagg
     1201 cgcggttcgc cgagagcacg tgcaggtcct cggagagcag cagcaggccg gcgggcacgc
     1261 tcgccacgat cttgtcggcg tagccctgca ggttcgtcat cgcgcgggcc tggtgcatcc
     1321 gatcctggag cagccgcgcg cgctgctgct cgcggtggag cgcgatgagg aagccggcca
     1381 gggccaggag caggaaggcg gcggccgcgc cggccagccg cccggagcgc tggaactcgg
     1441 cgagcgcctc gtcgcggtcg accttgagca cgagcgacca cccggtgggc acgacccggc
     1501 gcaccgcggc cagcgccggc acgccgcggt agtcgatcgt ctcgcccacc gcctcgtgcc
     1561 cctgggcggc tgctcgggcg agcgcgccca gagcgccgag cgagcgcttc ggtatctcgg
     1621 cgtcgggcga gatgggctga cgcagcgggg agagaaaggt gggcggctcg gcgtcgaggt
     1681 ccgccaggag cgtctcgccg gtgtgcgtcg gctccgcgtc gtcggcgagg agcgggtaga
     1741 gaccggcttc cggcttggcc ccgagggtga ccacacccag cagcggccgg gtgtcgccgt
     1801 cgaggccggc gggggcgccg tcggagaaga cgggcaccgt gaccacgagc aggcggcccg
     1861 cgccctcgcc gccgagctcg aagcgcggct tccggctccg cgccacccgc ccggcgtgct
     1921 ccgcggcctg ctccagcaga ccggcggcga gcccggtgtc cgaggcggag cgggccgccg
     1981 ggcggccggc cgcgtccgag atcacgatgc caacgtagcc gtacgcggcg gcgacccggc
     2041 tcaggtgccg cgcgagcgcc tcgtcgtccc ggccctggcc ggcgagctgc gcgcgcaccg
     2101 cggggaatgc ggccagcact tccgcgtcgg cgcgccgggc cgcgagccag ctcgacacga
     2161 ggcgggtccg ctcggccgcg atggtggcga ggcgggtctt ccagtgggtg agggcggtct
     2221 cgcgctcggt gccgatctgg tgcgaggtga acagcacgac caggatggtg ccgaccagaa
     2281 acccgccgag gtggatccag gggatgctct cgggggaccc cgtgccgcga tcggcgctcg
     2341 cccgcgcgtg ggaactctcg cggacgccgc taagaatgac ggaacctccg ccgtagtcgc
     2401 gtcatagtcg cgcacgtcca ggatacggcg ggcggcgggt cgcgacaacc tttcccgggc
     2461 ccggggcggg gcgggatcag acgtggttgc ggagcatcag ctccttcggg tgcggcctca
     2521 gatagatctg gtcgcggata taggccacgt cgaagcgcgc gacgtagtgg ttgatcagcg
     2581 tgatgggcac caccaggggc acgagcccgg cggcgtagtc gccgatgacc ccgagcagct
     2641 cctgcttctc ggcggccgcg agctgcttct tgaagaagcc catgatgtgc tgcaggacgt
     2701 tgacgtgctt cttgatcgtc gcccgcgcct cgagcgcctc catgaagcct tcaccgtagg
     2761 cgtcgagcca tcgcgcgcgc ggcgggcggg cgacctccgc caccaggcgg cccagccggg
     2821 cgtaggcggc cgggctgtgc gcgagcagca ggtacttgtg cgcggtgtgg aactccacca
     2881 ccgcgcccgg ccgcggctcg gactcccgca gcgcggcgag gcggcgatag gcgaagacgc
     2941 gggtgatgaa gttgtcacgc aggcgcgcgt cgttgagccg gccctcctcc tccaccggca
     3001 ggagcggcag ggcggcggtg agcgcgcgcg cgaagagccc ggtgcccgtc cgcgcgggcg
     3061 gcccgtcgct cgggtagacc tttacgcgct cgaggccgca ggagggcgag gcgcgcttca
     3121 ggatgtagcc gctcaggtcg cggcgccgga gctcgtccac gcgccgccgc gcgaactcgt
     3181 gcatgcgctc cgtgtgatcg gttccgctcg tgacgcccat caggcgcggc gcggccgcgt
     3241 cgccgaccag ccggatcggc tcgcgcggca cgcccatgcc gacctccagc tcggggcaca
     3301 ccggcaccca cgtgaagtag cgcgcgagca cgccggtgat gtaggcgtcc ttctggtggc
     3361 cgccgtcgta gcgtacttcc tcgcccagca ggcacgagga gatgccgacc cggatgggga
     3421 gatcgagcga gcgccagccc ggcgtggccg tcatcagcgg gctcggtagc ggacctcgaa
     3481 gctcacgcac cccgccgccg agtggaacgg cccgtgcggc atgccgggcg gccgtaccgc
     3541 caccgagccc gccgggtagg ggcggccgcc gcactcgagc gtgcccgaga tgatgtagat
     3601 ctcctcccag aagtcgtggg tcacgacgcg gtcgttcccg gagcccggat cgaagcggag
     3661 caggcgggtg gtgaccccct cgtcgtcccc gccgctgatg acctgctccc agacgccgga
     3721 ggggaatccg ggcgcggggc gccacggcag gggggccgga tcgaagaact cgcgctcggc
     3781 tttcgtggct ttcatcgctg gggtattgta gccgacatgc cgaccatcga ctccgcccgg
     3841 ctcgaggcga aggccacccg gatcttcacc gcgatgggcg cgccggacgg cgacgcggcg
     3901 tggatcgccg cgctgctcgt gcgcgcgaac ctgcgcggcc acgactccca cggcgtcatc
     3961 cggatccccc agtactggga gtcggtgaag aagaaggaag tggatccccg ctccccggtg
     4021 acggtcaccg cggagacgcc cgcgctggcg cggctcgacg gcggcggcgg cttcggccag
     4081 gtggtgggcc gccgcgcgat ggagatggcg atcgcgaagg cgaaggcggg cggcctgagc
     4141 gccgcgtcca cctcgcgcac cagccacgtg gggcggctcg ccgactacgc ggagatggcc
     4201 gccggcgccg ggctcgtcgg catgctctgg gccaactgcg tgcacgggct caacgtggcg
     4261 ccgtggggcg gcgccgcgcg acgcctcggc acgaacccgc acgcggtcgg catccccggc
     4321 gcgggcggcc ccgcgatggt gctcgatttc gccaccagcg tggtggccga ggggaagctg
     4381 cgcgtgaagc gcaaccgcaa gcagcccgct ccgcccggct ggttcgtgga cgcgcagggc
     4441 cgcccgcaga cggatcccga gatcttctac ggcaccccgc ccggcgcgct gctcaccgcg
     4501 ggcgagcaca agggctacgg gctctcgctc gcggtggaga tcctgggcgg catcctctcc
     4561 ggcaccggcc ccgcgggccc gccgcccggc gtcttcgcca acggcacgct catgatctgc
     4621 ctcgacgtgg agcgcttcct gccgctgccc gagttccacc gccaggtggc ctcgctcttc
     4681 gggtgggtga agtcggcgcc gctcgcgcag ggcgccaccg agatcctgat cccgggcgag
     4741 ccggaggcgc ggctcgaggc ggagcgccgc cggcagggga ttcccatcga ggatcagacc
     4801 tggagtcaga tcgagacgac ggcggcggag ctgggcctct aggcccaagc cggtctacgg
     4861 aaagagccgc tcggcgttcc tgaaggcgat cttctcggcc acgtcgcggg ggagccggct
     4921 caggtagctc cgcacctcgg tcaccgagcc cggcagcgcg tcccaccgcg acgtcatcca
     4981 ggtatcggtc ccggcgagga agcggtcggg atggcgaagg aacaccgcgc gccagcccgg
     5041 gtcgagcgtg ccgcccgggg ccacgtcact gttgcggatg gccaggtcca cccagagggt
     5101 cgggtagcgg tcgagcaggg cgcccaccgc ctgcgggccc gaggacatgc ccgcgtgcgc
     5161 ccagat
//
LOCUS       DASUFA010000018         6276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_805812, whole genome shotgun sequence.
ACCESSION   DASUFA010000018 DASUFA010000000
VERSION     DASUFA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805812"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2234)
                     /locus_tag="VFX87_00650"
     CDS             complement(<1..2234)
                     /locus_tag="VFX87_00650"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4993437.1"
                     /translation="MSDPITVQKFAIGQSVSRLEDPRLVQGLGRYSDDVSLPRQAYAV
                     VVRSPHAHARIRRIDPAAALRAPGVVAVLTGADLAADGLGHLPTDAARKRRDGGPAFS
                     TPRPALIRERVRHVGDPVALVVAASPEAAADAAELVAVDYEPLPAVTGAARATSGGAP
                     AVWDEAPDNVAFVWEAGSRDAVARAFAGAAHVTRLDFVVTRVAAAPMEPRAAVGEFDR
                     RSGRYTLHTGIQGPHGLRALLAERIFRVPHGQVRVVTSEVGGSFGMRSGIYPELVLVL
                     WAAKRLGRPVKWTSSRREGFLSDEHGRDNVTTAELALDAGGRFLGLRVALKVDIGAYL
                     TPRSAGPATNNVGGLAGVYTTPAIHIESTGVYTNTTPTGPYRGAGRPEATYAIERVID
                     VAARELGIDPVELRRRNLIPPSAMPFKTGLVFTYDCGDFERSMTMALTQSDRAGFERR
                     RAEARARGKLLGLGIANPIEVAGGPYTGVNPDTAELRVNPDGSVSLFAGSTSMGQGNE
                     TAFTQIVSDRLGLPPDRIQVFSGDSDALDAGRGNGGSGAISVGGSAVLRATEKTIERG
                     RRIAAHLLEAALEDVALRDGRFVVAGTDRDVPWSRVARTAYQSRALPPGLEPGWSDTA
                     AFTPPAVTFPNGCHVCEVEVDPETGAVRVLRYTVVDDVGRMINPLLVKGQIHGGAVQG
                     LGQGLAEVLTYDEAGQVLSGSFMDYAMPRADEVPLFDVDAHEVPTRVNPLGAKGVGEA
                     GTVGA"
     gene            complement(2328..3827)
                     /locus_tag="VFX87_00655"
     CDS             complement(2328..3827)
                     /locus_tag="VFX87_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993438.1"
                     /translation="MKTLRASVVLASLARIASAFSIPQPGESLPASSPALTGAPGSDR
                     APGHAPTPGRPLRRSLNAAVAEGAFAEIFAACATGAVITGWALYLGASAAVIGFLGAL
                     PLGAQVASLPAAWFTSARSRKAVALWAVGISRLTFLPLLALPTLDADPATKLDLFLGI
                     VAISTVASVIGNSAWVAWMGDLVPDRLRGRFFGRRTIFLSIAGTLTSLGAAILLDRLA
                     PIGWTGAALSGLTAAAFLAGVVSVVLMLRQHEPPVAAEPGMSAWQALGLTLHDRRARP
                     FLGYLFAWNAAVGISASFFAYHMLANLHTGFLVVAAHGVGVAAVRIASARFWGRAVDR
                     IGAGPVVVFCSFGIAAVPLTWFFVTPDRLWPIALDAIFAAFLWSGHNIAAFDLTIGLA
                     PRASRPFYLAAFATAGGLGFGLASMLAGQLAALVPVHFVLAGEAWTNLHVLFLLSAVG
                     RLASATLSLRLHDPGARGDVRELVRALADRTGLAAVRPYLPAFARLARR"
     gene            3984..4934
                     /locus_tag="VFX87_00660"
     CDS             3984..4934
                     /locus_tag="VFX87_00660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993439.1"
                     /translation="MRIGVLIPTRGAVMESARRPPVETCWTMARRADQAGYDAVWVGD
                     SIVAKPRLDALTTLAYLAGITTRVRLGTAVLLPALRHPVVLAQQLANIDQISQGRLVL
                     GLGVGWSLPSAEREWAACGANHKARVRRLEEHIAIWRKLWTGEPVTHHGDDLDLVDHT
                     IGPLPWRTEGPPVLITAGNRGELLPAQFERFGRLGDGIITTYVDFEEIPVIRERALDA
                     LARHGRPRADFPLCVYTTVRLDDDVATAQRVTTEFLTTYYGGGVHERGAMGLGPAAVV
                     IETIGRYAAVGVTDLCIRFIGNDQVAQLERFTREVLPAVR"
     gene            4997..5440
                     /locus_tag="VFX87_00665"
     CDS             4997..5440
                     /locus_tag="VFX87_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4993440.1"
                     /translation="MAEFPKLTYEALTVGEEFVSDTHLVTPEDIEAYAFAVDDHHPWF
                     SGPSPFGGPVAHPTLMANQALSLRHSKYIVHAGLHAKMEFNFLEPVRPGMRVRSRGRV
                     IDKYERRGRQYMVTEYVTEDETGAPLVRGQFTQMLIPEGTRHGDH"
     gene            5427..5837
                     /locus_tag="VFX87_00670"
     CDS             5427..5837
                     /locus_tag="VFX87_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4993441.1"
                     /translation="MAIIEALAVGQELPSLAKELTQRRIDVFSGVRPRSIHTDADWAR
                     QKGFRTTLAQGMMSTAYVSEMMTRLLGAGFVKGGTLSMAFIKPVYEGDRLTVHGVVKE
                     TRPEGAGTRVVVEVWCQNQHGEKTAVGTASGLRP"
     gene            complement(5834..>6276)
                     /locus_tag="VFX87_00675"
     CDS             complement(5834..>6276)
                     /locus_tag="VFX87_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4993442.1"
                     /translation="DVNDARLELARSLGADAVVDARSGPFHEAIRKLTGGQGVDVVLE
                     FVANPQTLPSSYQSVKRAGRLVFVGYTPGLPLPVMPHELVRNELEIVGSRANSKQELA
                     ETMELVAQGRIKPIVDRVIPLADIEDAFTALREGRPLGRNVLAI"
BASE COUNT          879 a         2197 c         2421 g          779 t
ORIGIN      
        1 gcccccaccg tgccggcctc gcccacgccc ttggcgccca gcgggttcac ccgggtcggc
       61 acctcgtggg cgtcgacgtc gaagagcggc acctcgtcgg cccgcggcat cgcgtagtcc
      121 atgaacgagc cggagagcac ctggccggcc tcgtcgtagg tgaggacctc ggccaggccc
      181 tggcccaggc cctggacggc gccgccgtgg atctgcccct tgacgagcag cgggttgatc
      241 atgcggccga cgtcgtccac gacggtgtag cgcagcacgc ggaccgcgcc ggtctccggg
      301 tccacctcga cctcgcagac gtggcagccg ttggggaagg tgacggcggg cggagtgaag
      361 gccgccgtgt cgctccagcc cggctcgagg ccgggcggca gggcgcgcga ttgataggcg
      421 gtgcgggcca cgcggctcca cggcacgtcg cggtcggtgc ccgccacgac gaagcggccg
      481 tcgcgcagcg cgacgtcctc cagcgccgcc tccagcaggt gcgcggcgat gcgccggccg
      541 cgctcgatcg tcttctcggt ggcgcgcagc accgcggagc cgccgaccga gatggcgccc
      601 gatccgccat tgccccggcc cgcgtcgagc gcgtcgctgt cgcccgagaa gacctggatg
      661 cggtcgggcg gcaggccgag ccggtcgctc acgatctggg tgaacgccgt ctcgttgccc
      721 tggcccatgg aggtcgagcc cgcgaagagc gagacggagc cgtcggggtt gacgcgcagc
      781 tccgcggtgt cgggattgac cccggtgtag gggccgcccg cgacctcgat cgggttcgcg
      841 ataccgagcc cgaggagctt gccgcgcgcc cgcgcctcgg cccggcgccg ctcgaagccc
      901 gcgcggtcgg actgggtcag ggccatcgtc atgctccgct cgaagtcgcc gcagtcgtag
      961 gtgaagacga ggccggtctt gaagggcatc gcggagggtg ggatcaggtt gcggcggcgc
     1021 agctcgaccg ggtcgatccc gagctcgcgc gccgccacgt cgatgacgcg ctcgatcgcg
     1081 taggtcgcct cggggcgccc ggctccgcgg tacgggcccg tcggcgtggt gttcgtgtac
     1141 acgccggtgc tctcgatgtg gatggcgggc gtggtgtaca cgcccgcgag gccgcccacg
     1201 ttgttggtgg cggggcccgc gctgcgcggc gtgagatacg cgccgatgtc caccttgagc
     1261 gcgacgcgca ggccgaggaa gcggccgccc gcgtcgagcg ccagctcggc cgtggtcacg
     1321 ttgtcgcggc cgtgctcgtc gctgaggaag ccctcgcggc ggctcgacgt ccacttcacc
     1381 ggccggccca ggcgcttcgc ggcccagagc accagcacca gctccgggta gatgccgctg
     1441 cgcatgccga agctgccgcc gacctcgctg gtgaccacgc ggacctgccc gtggggcacg
     1501 cggaagatcc gctcggcgag cagcgcgcgg aggccgtgcg gtccctggat gccggtgtgg
     1561 agcgtgtagc ggccgctgcg gcgatcgaac tcgccgaccg cggcgcgcgg ctccatcggc
     1621 gcggcggcca cgcgcgtcac cacgaagtcc agccgcgtga cgtgggcggc gcccgcgaag
     1681 gcgcgcgcca ccgcgtcgcg ggagcccgcc tcccagacga aggccacgtt gtcgggcgcc
     1741 tcgtcccaga cggccggcgc gccgccgctc gtggcgcgcg cggcgcccgt caccgcgggc
     1801 aggggctcgt agtcgacggc gaccagctcc gcggcgtcgg cggccgcttc cgggctcgcg
     1861 gccaccacga gcgccaccgg atcgcccacg tggcggacgc gctcgcggat cagcgcgggc
     1921 cgcggggtcg agaacgcggg cccgccgtcg cggcgcttgc gcgcggcgtc ggtcggcagg
     1981 tggccgaggc cgtcggccgc cagatcggcg cccgtgagca ccgcgaccac gccgggcgcc
     2041 cgcagcgcgg cggcggggtc gatgcggcgg atccgcgcgt gcgcgtgcgg cgagcgcacc
     2101 accaccgcgt aggcctggcg cgggaggctc acgtcgtcgg agtagcggcc gagcccctgg
     2161 accaggcgcg ggtcctccag gcgggagacg gactggccga tcgcgaattt ctggacggtg
     2221 atcgggtcgc tcatgggggc gggtcgctac tatgccacgc ggggagtggg cgggcaaacg
     2281 gcggggcccc tcaccctgcc ctctccccgg aggggagagg gtttcaatca gcggcgggcg
     2341 agtcgggcga acgcggggag gtaggggcgg acggcggcga ggccggtgcg gtctgccagg
     2401 gcgcggacca gctcgcggac gtcgccgcgg gcgcccgggt cgtgcaggcg gagcgacagc
     2461 gtggcggagg ccaggcggcc caccgcggag agcaggaaga ggacgtgcag gttggtccac
     2521 gcctcgccgg ccagcacgaa gtggacgggg acgagcgcgg cgagctggcc ggccagcatc
     2581 gaggccaggc cgaagcccag gccgcccgcg gtggcgaagg cggcgaggta gaacgggcgc
     2641 gaggcgcgcg gggcgaggcc gatggtcagg tcgaaggcgg cgatgttgtg gcccgaccag
     2701 agaaaggcgg cgaagatcgc gtccagcgcg atcggccaga ggcggtccgg cgtcacgaag
     2761 aaccaggtga gcggcaccgc cgcgatgccg aaggagcaga agaccacgac ggggccggcg
     2821 ccgatgcggt ccaccgcgcg gccccagaac cgcgccgagg cgatgcgcac cgcggccacg
     2881 ccgacgccgt gggcggccac caccaggaag ccggtgtgga ggttcgcgag catgtggtag
     2941 gcgaagaagc tggccgagat acccaccgcc gcgttccagg cgaacaggta gcccaggaag
     3001 gggcgcgcgc gccgatcgtg cagcgtgagc cccagcgcct gccatgcgga catgcccggc
     3061 tcggcggcca cgggcggctc gtgctggcgg agcatcagca ccacgctgac cacgccggcc
     3121 aggaacgcgg cggcggtgag ccccgagagc gcggcgccgg tccagccgat cggcgcgagc
     3181 cggtccagca ggatcgcggc gccgagcgag gtcagcgtgc ccgcgatgct gaggaagatc
     3241 gtccggcgcc cgaagaagcg cccgcgcagg cggtccggca cgaggtcgcc catccacgcc
     3301 acccaggcgc tgttgccgat cacgctcgcc accgtgctga tcgcgacgat gccgaggaag
     3361 agatcgagct tcgtggccgg gtcggcgtca agcgtcggaa gcgcgaggag tggcaagaac
     3421 gtcaggcgcg agatcccgac ggcccagagg gccaccgcct tgcggctgcg cgcgctcgtg
     3481 aaccaggcgg cgggcaggct cgccacctgg gcgccgagcg gcagcgcgcc caggaagccg
     3541 atcaccgccg cgctcgcgcc caggtagagc gcccagccgg tgatgacggc tccggtggcg
     3601 caggctgcaa aaatttccgc gaaggcgccc tccgccacgg cggcgttcag cgagcggcgc
     3661 agcgggcggc cgggcgtcgg ggcgtgaccc ggcgcgcggt ccgatccggg cgcgccggtc
     3721 agcgcggggc tcgaggcggg gagcgattcg ccgggctgcg ggatcgagaa ggcggaggcg
     3781 atgcgggcga gcgaggccag cacgacggag gcgcggagcg tcttcaaggc atcattcgta
     3841 attgcacgtc gcgggccatc gcgtggcgct tgatttggcg ggggttctcg cgcgcgcgcg
     3901 atccgcgagg gccggcggag cgacgtccgc cgtcagccga ctgacacgga tcggcgggcc
     3961 ccggatgagg tagactcccg cccatgcgaa tcggcgtcct cattcccacg cgtggcgcgg
     4021 tgatggagtc ggcgcggcgg ccccccgtcg agacctgctg gaccatggcc cgccgggccg
     4081 atcaggccgg ttacgacgcg gtctgggtgg gcgacagcat cgtggccaag ccccggctcg
     4141 acgccctgac gaccctggcc tacctggcgg ggatcaccac gcgggtgcgc ctgggcacgg
     4201 cggtcctgct cccggcgctg cgtcacccgg tggtgctggc ccagcagctg gccaatatcg
     4261 accagatctc gcagggccgg ctggtgctgg ggctgggcgt gggctggagc ctgcccagcg
     4321 ccgagcgcga gtgggcggcg tgcggcgcca accacaaggc ccgggtgcgc cggctcgagg
     4381 agcacatcgc gatctggcgg aagctctgga cgggcgagcc ggtgacccat cacggcgacg
     4441 acctcgacct cgtggaccac accatcgggc cgctgccgtg gcggaccgag ggcccgcccg
     4501 tcctgatcac cgcgggcaac cgcggggagc tgctgcccgc gcagttcgag cgcttcggcc
     4561 ggctcggcga cgggatcatc acgacctacg tggacttcga ggagatcccg gtcatccgcg
     4621 agcgcgcgct cgacgccctc gcgcggcacg gccggccccg cgcggacttt cccctctgcg
     4681 tgtacacgac ggtgcggctc gacgacgacg tggccaccgc ccagcgcgtc accaccgagt
     4741 tcctgaccac ctactacggc ggcggcgtgc acgagcgggg cgccatgggc ctggggccgg
     4801 cggcggtcgt gatcgagacc atcgggcggt acgcggcggt gggcgtgacc gatctctgca
     4861 ttcgattcat cggcaacgat caggtggcgc agctcgagcg gtttaccaga gaggtgctgc
     4921 cggcggtgcg gtagccccgt caccacccct caccctgccc tctcccctga ggggagaggg
     4981 ttgtcggagg agagacatgg ccgagtttcc gaagctgacg tacgaggcgc tgacggtcgg
     5041 cgaggagttc gtgtcggaca cgcacctggt gacgccggag gacatcgagg cctacgcctt
     5101 cgcggtggac gaccatcatc cgtggttctc ggggccgtcg cccttcggcg ggccggtggc
     5161 gcaccccacg ctgatggcca accaggccct gtcgctgcgc cacagcaagt acatcgtgca
     5221 cgcggggctg cacgccaaga tggagttcaa cttcctcgag ccggtgcggc ccggcatgcg
     5281 ggtgcgctcg cgcggccggg tcatcgacaa gtacgagcgg cgcggccgcc agtacatggt
     5341 caccgagtac gtcaccgagg acgagacggg cgccccgctc gtccgggggc agttcaccca
     5401 gatgctgatc ccggagggca cgcgccatgg cgatcattga ggccctcgcg gtcgggcagg
     5461 agctgccgtc gctcgcgaag gagctgacgc agcggcggat cgacgtcttc tcgggcgtgc
     5521 gcccgcgctc catccacacc gacgcggact gggcgcggca gaaggggttc cgcaccacgc
     5581 tggcgcaggg gatgatgtcg acggcctacg tctcggagat gatgacgcgc ctcctcggcg
     5641 cgggcttcgt caagggcggc accttgtcga tggcgttcat caagccggtg tacgagggcg
     5701 accggctgac cgtgcacggg gtggtgaagg agacgcggcc cgagggcgcg gggacgcgcg
     5761 tggtcgtgga ggtctggtgc cagaaccagc acggcgagaa gaccgcggtg gggacggcca
     5821 gcggcctccg gccctagatc gccagcacgt tgcggcccag cggccgccct tcacggagcg
     5881 cggtgaacgc gtcctcgatg tcggccagcg ggatcacgcg gtccacgatc ggcttgatgc
     5941 gtccctgagc caccagctcc atcgtctccg cgagctcctg cttgctgttg gcgcgggagc
     6001 cgacgatctc gagctcgttg cggaccagct cgtgcggcat caccggcagc ggcagccctg
     6061 gcgtgtagcc gacgaacacc agccgcccgg cccgcttcac gctctggtag ctcgacggca
     6121 gcgtctgcgg gttggcgacg aactccagca ccacgtccac gccctggccg ccggtcagct
     6181 tccgtatcgc ctcgtggaaa ggcccgctgc gcgcgtccac caccgcgtcg gcgcccaggc
     6241 tccgggccag ctcgagccgc gcgtcgttga cgtcca
//
LOCUS       DASUFA010000019         5121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_577029, whole genome shotgun sequence.
ACCESSION   DASUFA010000019 DASUFA010000000
VERSION     DASUFA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577029"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1592)
                     /locus_tag="VFX87_00680"
     CDS             complement(201..1592)
                     /locus_tag="VFX87_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168061264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEX4993443.1"
                     /translation="MREPGDVVLVACYELGHQPLAVAWPAAFLERAGYRPALMDVSVE
                     PVDAQKLARAQVIAISVPMHTALRLGLEVAARARAENPGAHLCFFGLYATLNADYLFA
                     HGADSVIGGETEAPLVALVDALERGDTGPLPAVARPGQPAAPNLTRLDFPVPSRAGLP
                     SIKAYTHVERDGRMDLVAYAESSRGCLHRCRHCPIPPVYGGRFFAVPREIVLADIRQQ
                     VEAGARHVTFGDPDFLNGPTHALAVARELHAEWPDVTFDVTAKIEHLLRHRARLPALA
                     AAGCLFIVSAAESLSDTVLAHLEKGHTRADILTALAATREAGITLRPTWVAFTPWTTL
                     DDHRAWLDFLETEGLIDHVDPVQYGLRLLVPPGSLLLETEALRPHLGPLEQETLSYRW
                     THPDPRMDRLQAETAELVARATEMKADAPAIFDRVRAMAAAAAGATAPAPVAGGMAKG
                     RKRPPRLTEAWFC"
     gene            complement(1603..2781)
                     /locus_tag="VFX87_00685"
     CDS             complement(1603..2781)
                     /locus_tag="VFX87_00685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993444.1"
                     /translation="MSPRWAMLLVIFFTRTSLGFQFQSIAALTPFLVTAFDISWAQVG
                     LLMGLFMLPGVVFALPGGLLGQRFGSRRVVFAGLALMIAGGMLVSYAGGFTSAALGRT
                     LAGIGGVLVNIMLARMVADWFRGRELQTSLGLMLAAWPFGMALALMMLGPLALIASWR
                     VAEYATVAAAGLALAVMALVYRDPPGAGGGEAARLRLNVPRRVWVLAVSAGVGWALLN
                     ASIIVVASFAPSFLMSRGASVSEAGLITGAALWISIVSVPVGGLVADRVNRPKLLIVS
                     GCLVAAVSMVAIACAPMPAVWMVVTGILLGLPPSIMMALLPRAVPAEHLATALGVFYG
                     LFYLGMAVSQTLAGLLRDLTGDPAVPLLFAAALMALAVPAVALFWRIEARTDRSDPGV
                     "
     gene            complement(2781..3164)
                     /locus_tag="VFX87_00690"
     CDS             complement(2781..3164)
                     /locus_tag="VFX87_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993445.1"
                     /translation="MTQVQVAVSLTAFGFAPRPGTAVVIEQSTGERVARVFTGLGMFW
                     ALALGGLFIPVAHFILVPAFLTAGIIMAVKRSREDRRLAEVRGPCPRCGAQQTFKAGG
                     RFEAGRSLDCPACHGTFALAVPEGA"
     gene            complement(3180..4277)
                     /locus_tag="VFX87_00695"
     CDS             complement(3180..4277)
                     /locus_tag="VFX87_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX4993446.1"
                     /translation="MTQALALLLALLAWPAAAGAQNEPPLKVPAGFAIEVFAAKVGSP
                     RFMALDPAGTLLVSEPARGRVLALPDRNGDGKADGIQTVVDGLDQPHGLAFRDGALYV
                     AENGRVQRFRYDPATMKATQPTVVVPGLPAGGGHWTRTVVFGPDGAMYVSVGSSCNVC
                     RESDKRRAAVLRYNADGSGERIFASGLRNAVGLAFQPGSGALWATVNERDWRGDDVPS
                     DMITEVRDGAFYGWPDCLTVGGKAIPDDRFEKGARCDRVTPPTVAIQAHSAPIGLVFY
                     TGTQFPAEYRGSLFVAYRGSWNRTVPTGYKVVRVRFRDGKPAGVEDFATGWLEGASSW
                     GRPVDLIVGRDGALYLSDQGAGRIYRISHRA"
     gene            complement(4274..>5121)
                     /locus_tag="VFX87_00700"
     CDS             complement(4274..>5121)
                     /locus_tag="VFX87_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4993447.1"
                     /translation="YSPLPARPPWTLPKGARIAVWTIVNIEEWDIEKAMARQYLTAPQ
                     GVATVPDVPNWAWHDYGMRVGFWRLLEALKKRKIRATTAINAHVVESYEPVARAMLDA
                     GWEFMAHGVVQGAMHLLPDQPAAIRQAIELLQKFTGKKPKGWLGPGLTETWETLDYLA
                     AEGIEYVSDWVNDDQPYEIRTKAGPLVSVPYTLELNDIPMMVIQHHGSEAWLQRAKDQ
                     FDRLYAEGARNPRVMALAVHPYISGVPHRIKYFEAVYDYMRKKPGVWFTTGEEIYEWY
                     RRERK"
BASE COUNT          764 a         1856 c         1861 g          640 t
ORIGIN      
        1 gaccggccgg cgcgaatcga gaccatgtcc ttcatggccg cgacgtgatg cggcactccg
       61 gccgcgctcg ccttgccggg gctctgcagc gtcgcggaga cgttgatcag gtcaatcctg
      121 gccatgggcc cctccgcccg gatgcgagtc tacagcgggt tccgcgtcgt cagggccaac
      181 tggccccggg tgggctccgc tcagcagaac cacgcctcgg tgaggcgggg cggacgcttg
      241 cggcccttgg ccatgccgcc cgccaccggc gcgggcgcgg tggccccggc cgccgccgcg
      301 gccatcgccc gcacgcggtc gaagatggcg ggcgcgtcgg ccttcatctc ggtggcgcgg
      361 gccaccagct ccgcggtctc cgcctgcagg cggtccatgc gcgggtcggg atgggtccac
      421 cggtacgaga gcgtctcctg ctcgagcggg cccaggtgcg gccgcagggc ctcggtctcg
      481 agtagcagcg agcccggcgg caccagcagg cgcaggccgt actgcaccgg gtccacgtga
      541 tcgatcagcc cctccgtctc gaggaagtcg agccaggcgc gatgatcgtc gagcgtggtc
      601 cacggcgtga aggccaccca cgtgggccgg agcgtgatgc cggcctcgcg cgtggccgcg
      661 agcgcggtca ggatatcggc gcgcgtgtgg cctttctcca gatgcgcgag caccgtgtcg
      721 ctcagcgact cggcggccga gacgatgaag aggcagccgg ccgcggccag cgcgggcagg
      781 cgcgcgcggt ggcgcagcag gtgctcgatc ttcgcggtga cgtcgaacgt gacgtcgggc
      841 cactcggcgt gcagctcccg cgccaccgcg agcgcgtgcg tgggcccgtt gaggaagtcg
      901 ggatcgccga aggtcacgtg gcgggcgccc gcctcgacct gctggcggat gtcggccagc
      961 acgatctcgc gcggcaccgc gaagaagcgc ccgccgtaca ccggagggat cgggcaatgc
     1021 cggcagcgat gcaggcaccc gcggctcgac tccgcgtagg cgaccaggtc catgcggccg
     1081 tcgcgctcga cgtgggtgta cgccttgatc gagggcaggc ccgcgcggct cggcaccggg
     1141 aaatcgaggc gcgtgagatt cggcgccgcc ggctgccccg gccgcgccac cgcgggcagc
     1201 gggcccgtgt cgccccgctc gagcgcgtcc accagcgcga ccagcggcgc ctccgtctcg
     1261 ccgccgatca cgctgtcggc cccgtgggcg aagaggtagt cggcgttgag cgtggcgtag
     1321 aggccgaaga agcagaggtg cgcgcccggg ttctccgcgc gggcgcgcgc ggcgacctcc
     1381 aggcccaggc gcagcgcggt gtgcatcggc accgagatgg cgatgacctg cgcccgcgcg
     1441 agcttctgcg cgtccaccgg ctccaccgag acgtccatca gcgccggccg gtaccccgcg
     1501 cgctccagga aggcggccgg ccaggccacc gcgagcggct ggtggcccag ctcgtagcag
     1561 gccacgagga caacgtcgcc cggctcgcgc atcccggcca gtctacaccc ccggatcgct
     1621 ccgatcggtc cgcgcctcga tgcgccagaa gagcgcgacg gcagggacag cgagggccat
     1681 cagcgcggcc gcaaacagta agggcaccgc ggggtcgccg gtcaggtccc gcagcagccc
     1741 ggccagggtc tgcgagacgg ccatgccgag atagaagagg ccgtagaaca cgccgagcgc
     1801 ggtggccagg tgctcggcgg ggaccgcgcg gggcaggagc gccatcatga tgctgggcgg
     1861 caggccgagc aggatgccgg tcaccaccat ccacaccgca ggcatgggcg cgcaggcgat
     1921 ggcgaccatg ctgaccgccg ccaccaggca gccgctgacg atcagcagct tcggccggtt
     1981 cacgcggtcc gccacgaggc cacccaccgg caccgacacg atgctgatcc agagcgcggc
     2041 gccggtgatc aggcccgcct cgctcacgga ggccccgcgc gacatgagaa agctcggcgc
     2101 gaagctcgcc accacgatga tgctggcgtt gagcagggcc cagccgacgc ccgcgctgac
     2161 cgccaggacc cacacgcgcc gcggcacgtt gagccgcagc cgcgcggcct cgccgccccc
     2221 ggcgccgggc ggatcccggt agacgagggc catcacggcc agcgcgagac cggccgccgc
     2281 cacggtggcg tactcggcca cccgccacga ggcgatcagc gcgagcggcc ccagcatcat
     2341 cagcgcgagc gccatcccga agggccacgc ggcgagcatc aggcccagcg aggtctgcag
     2401 ctcgcggccc cggaaccagt ccgccaccat ccgcgccagc atgatgttga ccagcacgcc
     2461 gccgatgccg gccagcgtgc ggccgagcgc ggcgctggtg aacccgcccg cgtaggagac
     2521 gagcatgccg ccggcgatca tcagcgcgag gccggcgaag acgacgcgcc ggctgccgaa
     2581 gcgctggccc agcaggccgc cgggcagcgc gaagaccacg cccggcagca tgaacaggcc
     2641 catcagcagc cccacctgcg cccaggagat atcgaaggcg gtgacgagga acggggtgag
     2701 cgcggcgatg gactggaact ggaagccgag cgacgtgcgg gtgaagaaga tcaccagcag
     2761 catcgcccag cgcgggctca tcaggcgccc tccgggacgg ccagtgcgaa cgtgccgtga
     2821 caggcgggac agtcgaggct gcggcccgcc tcgaaccggc cgcctgcctt gaaggtctgc
     2881 tgcgcgccgc agcgcgggca ggggccgcgc acctcggcga gccgccggtc ctcccgcgag
     2941 cgcttgacgg ccatgatgat gcccgcggtg aggaaggcgg gcaccaggat gaagtgcgcg
     3001 accgggatga agaggccgcc cagggccagc gcccagaaca tgccgagccc cgtgaagacg
     3061 cgggccaccc gctcgccggt ggactgctcg atcaccaccg cggtgccggg ccgcggtgcg
     3121 aagccgaacg cggtgagcga gaccgccacc tggacttggg tcatggaaag gctgtgcggt
     3181 caggcccggt ggctgatgcg atagatgcgt cccgcgccct ggtcggacag gtagagcgcg
     3241 ccgtcgcgcc ccacgatgag atccaccggc cgcccccacg acgaggcccc ctcgagccag
     3301 ccggtggcga agtcctctac ccccgccggc ttgccgtcgc ggaagcgcac gcgcaccacc
     3361 ttgtagccgg tgggcaccgt gcggttccac gagccgcgat aggccacgaa gaggctgccg
     3421 cgatactccg ccgggaactg cgtgcccgtg tagaagacga gcccgatggg cgccgagtgc
     3481 gcctggatcg ccacggtggg cggcgtcacc cggtcgcagc gcgcaccctt ctcgaagcgg
     3541 tcgtccggga tcgccttgcc gcccaccgtc aggcagtcgg gccagccgta gaaggcgccg
     3601 tcccgcacct cggtgatcat gtcggacggc acgtcgtcgc cgcgccagtc gcgctcgttc
     3661 acggtggccc agagcgcgcc gctgccgggc tggaaggcca ggcccaccgc gttgcgcagc
     3721 ccggaggcga agatgcgctc gcccgagccg tcggcgttgt agcgcagcac cgcggcgcgg
     3781 cgcttgtcgc tctcgcggca gacgttgcag gaggagccca ccgagacgta catcgccccg
     3841 tcgggcccga agaccaccgt gcgcgtccag tgcccgccgc ccgcgggcag gcccggcacc
     3901 accaccgtgg gctgcgtggc cttcatcgtg gccggatcgt agcggaagcg ctgcacgcgg
     3961 ccgttctccg ccacgtagag cgcgccgtcg cggaacgcca ggccgtgcgg ctggtcgagc
     4021 ccgtccacca ccgtctggat cccgtcggcc ttgccgtcgc cgttccggtc gggcagggcc
     4081 agcacgcggc cgcgcgccgg ctccgagacc agcaaggtgc ctgccgggtc cagcgccatg
     4141 aagcgcgggc tgcccacctt ggccgcgaac acctcgatgg cgaagcccgc gggcaccttg
     4201 agcggcggct cgttctgcgc gccggcggcg gccggccacg cgagcagcgc cagcagcagg
     4261 gcgagcgcct gtgtcacttg cgctcgcgcc ggtaccactc gtagatctcc tcgccggtgg
     4321 tgaaccacac gccgggcttc ttgcgcatgt agtcgtagac cgcctcgaag tacttgatgc
     4381 ggtgcgggac gccggagata tacggatgca cggccagcgc catcacccgc gggttcctgg
     4441 ccccctcggc gtagagccga tcgaactggt ccttcgcgcg ctgcagccag gcttccgatc
     4501 cgtggtgctg gatcaccatc atcgggatgt cgttcagctc gagcgtgtag ggcaccgaga
     4561 cgagcggccc cgccttggtg cggatctcgt agggctggtc gtcgttcacc cagtcggaga
     4621 cgtactcgat gccctcggcg gccaggtagt cgagcgtctc ccacgtctcg gtgaggccgg
     4681 gcccgagcca gcccttcggc ttcttgccgg tgaacttctg caggagctcg atggcttgcc
     4741 ggatggcggc gggctgatcg gggagcagat gcatcgcgcc ctgaacgacc ccgtgggcca
     4801 tgaactccca gcccgcgtcc agcatggcgc gggccacggg ctcgtaggat tcgacgacgt
     4861 gggcgttgat cgcggtggtg gcccggatct ttcgcttctt gagcgcctcc agcagccgcc
     4921 agaaccccac ccgcatcccg tagtcgtgcc aggcccagtt gggcacgtcg ggcacggtgg
     4981 ccacgccctg gggcgccgtg aggtactggc gcgccatcgc cttctcgatg tcccactcct
     5041 cgatgttgac gatagtccac accgcgatgc gggcgccttt ggggagcgtc cacggcgggc
     5101 gggcgggaag cggcgagtag t
//
LOCUS       DASUFA010000020         6612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1035433, whole genome shotgun sequence.
ACCESSION   DASUFA010000020 DASUFA010000000
VERSION     DASUFA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035433"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFX87_00705"
     CDS             <1..279
                     /locus_tag="VFX87_00705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4993448.1"
                     /translation="ATLIQLAVSRSREFQADATGAQIAGQPWGLAKALEKLEVASKVA
                     PMDATPATAHLFIVNPLTGGGWTTLFSTHPPIAERVARLRAMARSQAR"
     gene            356..1069
                     /gene="ccmA"
                     /locus_tag="VFX87_00710"
     CDS             356..1069
                     /gene="ccmA"
                     /locus_tag="VFX87_00710"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX4993449.1"
                     /translation="MIAARELRKTYGSNSVIDGVSFELGAGQCLTLLGPNGAGKTTLL
                     RILATLLRPTAGSLSIGGADVLREPERARALIGMVAHGSYVYEDLTARENLRFWETMR
                     GGDSTVGRLDAALAQVELDGVADERVRTFSAGMKRRLGLARVLLGEARLLLLDEPFTG
                     LDRRGRKWVNEFLLGFKAAGGAAVVATHSFGGGLGVADRVAILGSGRLLMDRPAADLS
                     PEELQRLYESLTDSAEGGP"
     gene            1066..1743
                     /locus_tag="VFX87_00715"
     CDS             1066..1743
                     /locus_tag="VFX87_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX4993450.1"
                     /translation="MTYARRAWIILWKDLLIERRAKEHANALLFFCVLLLFLFQFALG
                     PDRERLAAAVPGLLWLGFILAGLLGLGRSFLGERENDCWEGLRLTPGDKSAIYLGKLA
                     GNCVLMTAVEIMLVVLFGVFYDVDLWTPAAPLALVLVLGTIGLAAVGTLFAAMTTQVR
                     ARELLFPVLLLPVQVPVLLATVSATQALVLGQPLADVAHWLKLLAGADLVYVTIGLLT
                     FEFVLEG"
     gene            1748..2389
                     /gene="ccsA"
                     /locus_tag="VFX87_00720"
     CDS             1748..2389
                     /gene="ccsA"
                     /locus_tag="VFX87_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX4993451.1"
                     /translation="MIRALGWLSVLGLVAGLAAGFGYAPREAVQGNVQRIMYLHVPAV
                     LTAYLAFVLVLAGSIGYLATRRAGWDRLATAAGEIGVLFTGLTIVSGSIWGKPTWGTW
                     WTWDARLTSTAVLFCVYVGYLLLRSLIEDPEARARYAAVVGILGAVNIPIVHFSVKWW
                     RALHQPSTILGPERSPIDPAIGLALFVNWVAFTLLFAYFLSRRMEIARLEEQT"
     gene            2389..2523
                     /locus_tag="VFX87_00725"
     CDS             2389..2523
                     /locus_tag="VFX87_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993452.1"
                     /translation="MPSNWGYVLAAYGIAAVAFLSYWRHLARRTRALAARRAAKPRAA
                     "
     gene            2520..2966
                     /locus_tag="VFX87_00730"
     CDS             2520..2966
                     /locus_tag="VFX87_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX4993453.1"
                     /translation="MNRKTKFLAGGLVIVAALAYLVYAGVTQSVVYFVTPSELGAAPV
                     AGKAYRLGGMVQPGTLVWNPKSLDLRFTLSDGQATVPVRHRGTPPDLFAESRGAVVEG
                     TWSRDGYFQASTILAKHSEEYKAPHDTSQAGYKDLLKTLRSGEGKP"
     gene            2963..4942
                     /locus_tag="VFX87_00735"
     CDS             2963..4942
                     /locus_tag="VFX87_00735"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX4993454.1"
                     /translation="MTPELGYALTVGALVLALYGAGAAALSAVKGRPDLQRSSERAAI
                     GVCLLITSCMLLLVYAFLSFDFSVRYVASNTNRGTPFYYRITALWGALEGSIILWAWM
                     LSLYTLIMVGRYRRTQPQLYPWTLAVMLGISAFFLLVMTIPAPPFERLTPIPADGRGL
                     NPLLEDSGMITHPVALYLGFTGFTVPFAFAMAALIVGRTGDEWITITRRWTIIAWYFL
                     SLGLLIGGWWSYHVLGWGGYWAWDPVENAAFMPWLTATAFLHSVMIQERRRMLKLWNL
                     TLIILTFSLTLFGTFLTRSGIIGSVHAFTQGSIGIFFLVYLALVMLGAFSLLAWRLER
                     LRGQSELDSIFSRESAFLLNNLFLIAAAFTVFFGTVFPLLSEAVKGQKVSVGAPFFNL
                     VNIPIFLALIFLMGVGPLIAWRRASPENLRRNFLMPVVIGVVGAALCRALGVGNLLVL
                     LCMGLVVFVAATIALDFWRAARARRRTGDGWLDATWGLALRQNRRYGGFVVHLGILVV
                     ALGVAGSQAWSTQTEATINRGATVELAGYSIRFDDLQPVEESNHFKVVGTFTVSDRRG
                     PYTVLTPAKKFYPQEQTPIAAVDYRLGFMEDLYLVLGDFARDGSHATVKVQINRMVSW
                     LWIGGLILTLGAALAIVPEPRRTAPRRRPDRAVVA"
     gene            4939..5478
                     /locus_tag="VFX87_00740"
     CDS             4939..5478
                     /locus_tag="VFX87_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4993455.1"
                     /translation="MSARSRALRWVIPLAVVPVLVLLAYGFRTDPREIPSPLVGKPAS
                     PFALTAFDGKPVSLEALRGKIVIVNFWASWCVPACYDEAPSLERAWQAYRDRGVVLIG
                     VNIQDKEEPARRFLTQFGHTFPNAPDPAGRVSVDYGVYGVPETFFIDRKGRVRFKQVG
                     AVTDEIMKQQVEKLLAEPV"
     gene            5475..5936
                     /locus_tag="VFX87_00745"
     CDS             5475..5936
                     /locus_tag="VFX87_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX4993456.1"
                     /translation="MSRALALTLLLIAALAGASPLGAAPIREETVHEIGAQLRCVVCQ
                     SLSVADSPSETATQRRGIIRERLAAGDSPEQVKAYFVEKYGLWILLSPPRQGFNLLVW
                     VVPFAALGAGLVLVLLLVRRWSRRPGAAQAGPGPDAAMRARIHREMAEREP"
     gene            5933..>6612
                     /locus_tag="VFX87_00750"
     CDS             5933..>6612
                     /locus_tag="VFX87_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993457.1"
                     /translation="MTALQITLIAAIVAPALAIVLWPLLGGRAAAATSSAERASDDRR
                     LELEEEKSALYRALRELEFDHDAGHLSDPDYLSLRERYESRAASLITELDLIGGGPDR
                     APALPRPRTTVRPAERASWTRHPATLAAGAVALLIFGVIIGVNAGRFTERDESTMPPG
                     ARIPVPGPVPSPAGPPAARLEPGKPIPPEMLAGMLSAARQSLFEGRYPEAIAAYQAVL
                     KRDERNVDA"
BASE COUNT          821 a         2466 c         2232 g         1093 t
ORIGIN      
        1 gccaccctga tccagctcgc ggtctcgcgc tcccgggagt tccaggccga cgccaccgga
       61 gcccagatcg ccgggcagcc ctgggggctg gccaaggcgc tcgagaagct cgaggtggct
      121 tccaaggtgg cgccgatgga cgccaccccg gcgaccgccc acctcttcat cgtcaacccg
      181 ctcaccggcg ggggctggac cacgctgttc tcgacgcatc cgccgatcgc agagcgggtg
      241 gcgcggctgc gcgccatggc cagatcccag gccaggtagg tcgatcgtca gctcccacgc
      301 agtccacgca gcgggcccgg gggagacggg acggcgtctc ccctttttct cggacatgat
      361 cgcggcgcgg gagctccgga agacctacgg atcgaacagc gtgatcgacg gcgtgtcctt
      421 cgagcttggc gccggccagt gcctgaccct gctcggaccc aacggcgccg gcaagaccac
      481 gctgctccga atactggcca ccctgctccg tcccactgcc ggctcgctca gcatcggcgg
      541 cgccgacgtc ctccgcgagc ccgaacgggc gcgggcgctc atcggcatgg tagctcacgg
      601 ctcctacgtc tatgaggacc tcaccgcgcg ggagaacctg cgcttctggg agaccatgcg
      661 gggtggcgac agcacggtcg ggcggctcga cgccgcgctc gcccaggtgg agctcgacgg
      721 cgtggcggac gagcgcgtgc gcacgttctc ggcgggcatg aagcgtcggc tcgggctggc
      781 ccgcgtgctc ctgggcgagg cccggctgct cctgctggac gagcccttca cggggctcga
      841 ccggcgcggc cgcaagtggg tcaacgagtt cctgctgggc ttcaaggccg cggggggggc
      901 cgccgtggtc gcgacccaca gcttcggcgg cgggctcggc gtggccgacc gcgtggccat
      961 cctgggcagc ggccgcctcc tgatggacag gcccgccgcg gacctctccc ccgaggagct
     1021 ccagcgcctg tacgagagcc tcaccgactc ggccgagggc ggtccgtgac ctacgcccgg
     1081 cgcgcgtgga tcatcctctg gaaagacctg ctcatcgagc ggcgcgcgaa ggagcacgcc
     1141 aacgcgctcc tcttcttctg cgtgctgctg ctcttcctct tccagttcgc gctgggcccg
     1201 gatcgcgagc gcctggccgc cgcggtgccg ggcctgctct ggctgggctt catcctcgct
     1261 gggctgctcg gcctcggccg gagcttcctc ggcgagcgcg agaacgactg ctgggagggc
     1321 ctccggctca ctcccggcga caagtcggcg atctatctcg gcaagctcgc gggcaactgc
     1381 gtgctgatga ccgcggtgga gatcatgctg gtcgtcctct tcggcgtctt ctacgacgtg
     1441 gacctctgga cgccggcggc cccgctggcc ctggtgctag tcctcggcac catcggcctc
     1501 gccgcggtgg gcacactctt cgcggccatg accacccagg tgcgcgcgcg cgaactgctc
     1561 ttcccggtgc tgctgctgcc cgtgcaggtg cccgtgctgc tcgccaccgt gagcgcgacc
     1621 caggcgctcg tgctcggcca gccgctcgcc gacgtggccc actggctcaa gctgctggcc
     1681 ggcgccgatc tcgtctacgt gacgatcggg ctcctcactt tcgagttcgt gctggagggc
     1741 tgacgcgatg atccgtgcgc tcgggtggct gtccgtgctg ggcctggtgg ccggcctcgc
     1801 cgccggcttc ggctacgcac cccgcgaggc cgtccagggc aacgtgcagc gcatcatgta
     1861 tctccacgtg cccgcggtgc tcaccgccta cctggccttc gtcctcgtgc tggcgggcag
     1921 catcggctac ctggcgaccc ggcgcgccgg ctgggaccgc ctcgccaccg ccgcgggcga
     1981 gatcggcgtg ctcttcaccg ggctcaccat cgtctccggc tccatttggg gcaagccgac
     2041 ctggggcacc tggtggacgt gggacgcgcg cctcacctcg accgcggtgc tcttctgcgt
     2101 ctacgtcggc tatctgctgc tgcgcagcct gatcgaggat ccggaagcgc gggcgcgcta
     2161 cgcggcggtg gtcggcatcc tgggcgcggt caatattccg atcgtgcact tctcggtgaa
     2221 gtggtggcgg gcgctgcacc agccctccac gatcctgggg cccgagcggt ccccgatcga
     2281 tcccgcgatc ggcctcgcgc tcttcgtcaa ctgggtggcc ttcaccctgc tgttcgccta
     2341 cttcctgtcc cgccggatgg agatcgcccg gctcgaggag cagacctgat gccgtccaac
     2401 tggggctacg tgctcgccgc gtacggcatc gccgccgtcg cgttcctctc ctactggcgc
     2461 cacctggcgc gccggactcg tgcgctggcc gcgcggcggg ccgcgaagcc gcgggcggca
     2521 tgaaccgcaa gacgaagttc ctggcgggcg gcctggtcat cgtggccgcg ctggcctatc
     2581 tcgtgtacgc gggcgtgacc cagtcggtgg tgtacttcgt cacgccgagc gagctgggcg
     2641 ccgccccggt ggcgggcaag gcctatcgcc tgggcggcat ggtccagccc ggcacgctcg
     2701 tgtggaatcc caagagcctc gacctgcgct tcaccctctc ggacggccag gccacggttc
     2761 cggtgcgcca ccgcggcacc ccgccggatc tcttcgcgga gtcgcgcggc gcggtggtag
     2821 agggcacgtg gagccgcgac ggctacttcc aggcctcgac gatcctggcc aagcactcgg
     2881 aggagtacaa ggcgccgcac gacacgagcc aggcgggcta caaggacctg ctcaagacgc
     2941 tgcggagcgg cgagggcaag ccgtgacgcc cgagttgggt tacgcgctga cggtgggtgc
     3001 gctcgtgctg gcgctgtacg gggccggcgc cgccgcgctg agcgccgtca agggccggcc
     3061 cgatctgcag cgctcctcgg agcgcgcggc catcggcgtc tgcctgctga tcacctcgtg
     3121 catgctgctg ctggtctacg cgttcctgtc cttcgacttc tcggtccgct acgtggccag
     3181 caacaccaat cgcggcacgc ccttctacta ccgcatcacc gcgctctggg gcgcgctcga
     3241 ggggtccatc attctctggg cgtggatgct gtcgctctac accctgatca tggtgggccg
     3301 ctaccgccgc acccagccgc agctctaccc gtggacgctc gcggtgatgc tcgggatctc
     3361 cgccttcttc ctcctggtga tgacgatccc ggcgccgccc ttcgagcgcc tcactcccat
     3421 ccccgccgac ggccgcggcc tcaatccgct gctcgaggac tccgggatga tcacgcaccc
     3481 ggtggccctc tacctgggct tcaccggctt cacggtgccg ttcgccttcg cgatggctgc
     3541 gctcatcgtg ggccgcaccg gcgacgagtg gatcaccatc acccggcgct ggaccatcat
     3601 cgcctggtac ttcctctcgc tgggcctgct gatcggcggc tggtggagct accacgtgct
     3661 gggctggggc ggctactggg cgtgggaccc cgtggagaac gcggcgttca tgccgtggct
     3721 caccgccacc gcgttcctgc actcggtgat gatccaggag cggcggcgga tgctgaagct
     3781 ctggaacctc accctcatca tcctgacctt cagcctcacc ctcttcggca ccttcctcac
     3841 ccgctcgggc atcatcggct cggtgcacgc cttcacccag ggctcgatcg ggatcttctt
     3901 cctcgtatac ctcgccctgg tcatgctcgg cgccttctcg ctgctggcct ggcggctcga
     3961 gcggctgcgg gggcagagcg agctcgactc gatcttctcc cgcgagtccg ccttcctcct
     4021 gaacaacctc ttcctgatcg cggcggcctt caccgtgttc ttcggcaccg tgttcccgct
     4081 gctctcggag gcggtgaagg gccagaaggt gagcgtgggc gcgcccttct tcaacctcgt
     4141 gaacatcccg atcttcctgg ccctcatctt cctgatgggt gtgggtccgc tgatcgcgtg
     4201 gcgccgtgcc tcccccgaga acctgcgccg caacttcctg atgccggtcg tgatcggggt
     4261 agtgggcgcc gccctctgcc gcgccctcgg cgtcggcaat ctcctggtcc tgctctgcat
     4321 gggcctcgtg gtgttcgtcg ccgccaccat cgcgctcgac ttctggcgcg ccgcgcgcgc
     4381 ccgccggcgc accggcgacg gctggctcga cgccacctgg gggctggccc tccgccagaa
     4441 ccgccgctac ggcggcttcg tcgtgcacct gggcatcctg gtcgtcgcgc tgggcgtggc
     4501 gggctcgcag gcctggtcca cccagaccga ggccacgatc aaccgcgggg ccaccgtgga
     4561 gctggccggc tacagcatcc gcttcgacga tctgcagccg gtggaggagt ccaaccactt
     4621 caaggtggtc ggcaccttca cggtctccga ccgccgcggg ccctacaccg tgctcacgcc
     4681 ggcgaagaag ttctacccgc aggagcagac gccgatcgcg gcggtggact accggctggg
     4741 cttcatggaa gacctctacc tcgtcctggg cgacttcgcc cgcgacggct cgcacgccac
     4801 cgtcaaggtc cagatcaacc ggatggtctc ctggctctgg ataggaggcc tgatcctgac
     4861 tctgggcgcc gcgctcgcca tcgtgcccga gccccggcgg accgcaccgc ggcggcggcc
     4921 ggatcgcgcg gtcgtggcat gagcgcgcgc tcccgcgccc tgcgctgggt gatcccgctc
     4981 gcggtcgtgc cggtgctggt gctcctggcc tacggcttcc gcaccgaccc gcgcgagatc
     5041 ccctcgccgc tggtcggcaa acccgcctca cccttcgcgc tgaccgcctt cgacggcaag
     5101 ccggtgagcc tcgaggccct gcgcggcaag atcgtgatcg tcaacttctg ggcgtcgtgg
     5161 tgcgtgccgg cctgctacga cgaggcgccc agcctcgagc gggcctggca ggcctaccgc
     5221 gatcgcggcg tcgtcctgat cggtgtcaac atccaggaca aggaggagcc ggcccggcgc
     5281 ttcctcaccc agttcggcca tacctttccg aacgcgcccg accccgcggg gcgcgtctcg
     5341 gtggactacg gcgtctacgg cgtcccggag acgttcttca tcgatcgcaa gggccgggtg
     5401 cgcttcaagc aggtcggcgc cgtcaccgac gagatcatga agcagcaggt cgagaagctg
     5461 ctggcggagc cggtatgagc cgcgcgctgg ccctgaccct gctcctcatc gcggcgctcg
     5521 cgggagcctc tccgctcggc gccgcgccca tccgtgagga gaccgtccac gagatcggcg
     5581 cgcagctccg gtgcgtggtc tgccagagcc tctcggtggc cgactcgccg tccgagaccg
     5641 cgactcagag gcgcggcatc atccgcgagc ggctggccgc cggcgactcg ccggagcagg
     5701 tgaaggccta cttcgtggag aagtacggcc tctggatcct gctctccccg ccccggcagg
     5761 gcttcaacct gctggtgtgg gtggtgccgt tcgcggccct cggcgccggc ctggtgctgg
     5821 tgctcctgct ggtgcggcgc tggagccgcc ggcccggcgc ggcgcaggcc ggccccgggc
     5881 cggacgccgc gatgcgcgcg cgcatccatc gtgagatggc cgagcgcgag ccgtgaccgc
     5941 gctccagatc accctgatcg cggcgatcgt ggcgcccgcg ctcgcgatcg tgctgtggcc
     6001 cctgctcggc ggccgcgccg ccgcggccac gtcatcggcc gaacgcgcct ccgacgaccg
     6061 ccgcctcgag ctggaagagg agaagagcgc gctctatcgc gcgctgcgcg agctcgagtt
     6121 cgaccacgac gcgggccatc tctccgatcc cgactacctg agcctgcggg agcgctacga
     6181 gtcgcgcgcc gcctcattga tcaccgagct cgatctcata ggcgggggcc ccgacagggc
     6241 ccccgcgctc ccccggccgc ggacgaccgt gcggcccgcc gagcgcgcat cctggacgcg
     6301 gcacccggcc acgctcgcgg ccggcgcggt ggccctcttg atcttcggcg tgatcatcgg
     6361 cgtcaatgcg ggccgattca ccgagcgcga cgagagcacc atgccccccg gcgcccgcat
     6421 cccggtaccg ggcccggttc cctccccggc cgggcccccc gcggcgcggc tcgagccggg
     6481 caagccgatc ccacccgaga tgctggccgg catgctgagc gccgcgcgcc agagcctctt
     6541 cgagggccgc tacccggagg cgatcgccgc ctaccaggcc gtgctcaagc gcgacgagcg
     6601 caacgtggac gc
//
LOCUS       DASUFA010000021         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1035678, whole genome shotgun sequence.
ACCESSION   DASUFA010000021 DASUFA010000000
VERSION     DASUFA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035678"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFX87_00755"
     CDS             complement(<1..889)
                     /locus_tag="VFX87_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4993458.1"
                     /translation="MADAAGGSAAGHVVAVIGAGPAGLFAARALAAQGSRVLLLNRDV
                     KPGGLAEYGIFLNKYKMKSGLRRQFQKILTDPRVTYLGNVTVADGGALTVAELRGFGF
                     DALVFAIGAQGTKYLGIEGERLPGVYHAKDLVYHYNRLPPFSEQPFPMGRRVAIVGVG
                     NVMVDIANYCVHFCDCDAVIAVARRGPFEKAYDDREFEDVEDAFDRDLYRAEIARIRP
                     RLEAAAQDPDALLRALGQRPESESRTRARLSFRFLASPKRVVTEGGRVTGLEIEETRL
                     ERKGDRINAVGTGQTSVVPA"
     gene            1534..>2277
                     /locus_tag="VFX87_00760"
     CDS             1534..>2277
                     /locus_tag="VFX87_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4993459.1"
                     /translation="MTDEPTSGEPQPPVVQQSIAEISRTLEVLRERLQRLESGEAGSM
                     PEPPAGTDAVLPVDPKALAGIQEILSVPSSGFAPSEVFGLAMDRAARLLDADRAMLFV
                     VDPERSLLVPVAARGFRRDDLGGLAIPPGEGVVGRAFLEGRALLYTRPPETAPRDPFI
                     ARFPVRDALAVPVRADAQVVGVLFAGRRGRGVPFTAEDTVLLLVIADRVATAFAHQRL
                     VDRAAEHLARLRELQVFSGLAVVGRDLADS"
BASE COUNT          316 a          811 c          801 g          349 t
ORIGIN      
        1 cggccgggac cacactcgtc tggcccgtgc cgacggcatt gatccgatcg cccttgcgct
       61 cgagccgcgt ctcctcgatc tcgagacccg tcacgcggcc gccctccgtc acgacccgct
      121 tcggggaggc gaggaaacgg aaggacaggc gcgcgcgggt gcgcgactcc gactcgggac
      181 gctggcccag cgcgcggagg agcgcgtcgg gatcctgcgc ggcggcctcg agccggggcc
      241 ggatgcgggc gatctccgct cggtagagat cgcggtcgaa ggcgtcctcg acgtcctcga
      301 attcccggtc gtcgtacgcc ttctcgaagg ggccgcgccg cgcgaccgcg atcaccgcgt
      361 cacagtcgca gaagtggacg cagtagttcg cgatgtccac catgacattg cccaccccca
      421 cgatggccac gcggcggccc atggggaagg gctgctcgct gaagggcggc aggcggttgt
      481 agtggtagac gagatccttg gcgtggtaga cgccggggag ccgctcgccc tcgatgccca
      541 ggtatttcgt gccctgcgcg ccgatggcga acacgagggc gtcgaagccg aacccgcgca
      601 gctcggccac ggtaagggcg ccgccgtccg ccaccgtcac gttcccgaga taggtgaccc
      661 gcgggtcggt gaggattttc tggaactgcc gccgcagccc cgacttcatc ttgtacttgt
      721 tgaggaagat gccgtactcg gcgagcccgc ccggcttgac gtcgcgattg agcaggagga
      781 cgcgagagcc ctgggcggcg agcgcccggg cggcgaagag ccccgccggg ccggcgccga
      841 tcacggcgac gacgtgtccc gctgcgctgc ctcccgctgc gtccgccatg cgtgctccct
      901 ttcgatatcc gacgttggtt cgccgagccg cgcgattcga caccatagca caggggacca
      961 cgcgcggatc ggcgcgcgtc ccttgaccgc ttcggaagcc gtggtgtcta atggccccgg
     1021 ttgtgaacgt cgtcacgaca ctcacccgcg ccgcggttgc ggtgcgtcca ccgcacgctc
     1081 gggagaagag catgcatgag cgcttcacgg atcgccccat cgtgacggca gtcactcagg
     1141 ggaagccgcc cgcggagctc gaccgtgtga cctgtcgcgg gaccagggat ggagacgcca
     1201 gggcggcggc acccgcccga gatccccggc gcgccaaccg ctggaccatc acccactcca
     1261 tcgcctcact cgaaccgaaa tagctcgacc ttggaggctg gttcctcggg ccaacagtcg
     1321 aagaatctta cgatttcgcc gcgggtctcg gggcggtgcc ctggcgggcg gaaacggtcg
     1381 ccgtcggccc ttcaggaatc aatgggttag ccggatgcgt ccactcgcgg tggtttggca
     1441 tgggcgctgc tataccgact ggatctccat gaccatgccc ttgacgcgtc ggctcgccat
     1501 cgtcccccgc cggatgcccg cccggatcac ccgatgaccg acgaaccgac atcgggggag
     1561 ccgcagcccc cggtcgtcca gcagtcgatc gcggagatca gccggacact cgaagtgctt
     1621 cgcgagcggc tccagcggct cgagtccggc gaagcgggat cgatgcccga gccaccggcg
     1681 gggaccgacg ccgtgctgcc ggtcgatccc aaggcgctgg cgggtatcca ggaaatcctc
     1741 agcgtgccgt cgtcgggctt cgcgcccagc gaggtattcg gcctggcgat ggaccgcgcg
     1801 gcccgcctgc tcgacgccga ccgcgccatg ctgttcgtgg tggaccccga gcggagcctg
     1861 ctcgtcccgg tggccgcccg cggcttccgc cgcgacgatc tcggcgggct cgcgatcccg
     1921 cccggcgagg gcgtggtggg gcgggcgttt ctcgaggggc gcgcgctcct ctacacgcgg
     1981 cctccggaga ccgcgccgcg cgatcccttc atcgcccgct ttcccgtccg cgacgcgctc
     2041 gcggtccccg tccgcgccga cgcgcaggtg gtcggggtgc tcttcgcggg ccggcgcggc
     2101 cgtggcgtgc ccttcaccgc cgaggacacg gtgctgctcc tggtgatcgc ggatcgagtg
     2161 gccaccgcgt tcgcgcatca gcggctggtg gaccgggccg ccgagcacct ggcgcggctg
     2221 cgcgagctgc aggtgttctc gggcctggcc gtggtggggc gcgatctcgc cgactcg
//
LOCUS       DASUFA010000022         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_4966, whole genome shotgun sequence.
ACCESSION   DASUFA010000022 DASUFA010000000
VERSION     DASUFA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4966"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFX87_00765"
     CDS             complement(<1..424)
                     /locus_tag="VFX87_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX4993460.1"
                     /translation="MTEATGVTRRIVLPPDLNHLALLGRHDEHLRQLEAQYDVRITAR
                     GHDLTVRGEERQVLEAERVVRELVAMLRERPALGAADIRSALRIAGHEPGADVKSVLA
                     DAIAVPSRRRFITPKTANQKRYLEAVRGHDLVIAIGPAG"
     gene            complement(455..2245)
                     /gene="aspS"
                     /locus_tag="VFX87_00770"
     CDS             complement(455..2245)
                     /gene="aspS"
                     /locus_tag="VFX87_00770"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX4993461.1"
                     /translation="MTESLGGWRRTHSCGALRSLHVGQVVTLMGWAYRRRDHGGLLFI
                     DLRDREGLTQCVFNPGAGGGTHARAEAVRTEFVLAVRGTVSARPPGTENPKLATGGVE
                     VQVSEVRILNESRPLPFQLDDDAEIDETLRLKYRYLDMRRPHVLRAFQIRDQICRAAR
                     DYLHGEAFLEVETPFLTRSTPEGARDFLVPSRLQPGSFYALPQSPQLFKQLLMVAGFE
                     RYFQIVRCFRDEDLRKDRQPEFTQIDIETSFLDRDDFLPIIEGLVAEMWRRVKGVELA
                     RPFPRIPYDEAMGRYGSDKPDLRFGLELQDCSALFAGGEFQAFAQTVAAGGAVKGLRV
                     PAAGGMSRKELDDLTAEAKQAGAKGLVWIKVNPDGLQSPVAKFIQGIQARLLDTLGGV
                     AGDLLLLVADTPAVAATVLGRLRVDMARRFNLIPTDRDVFAWIIDFPLVEWNPDEKRW
                     DAVHHPFTAPRDEDLPLLESDPGRARAKAYDLVLNGQEAAGGSIRIHQPTVQERVFGL
                     IGISKDEARARFGFLLEALEFGAPPMGGIAFGLDRLAANMAGQESIREVIAFPKTQKG
                     TCPLTDAPAPVDPKQLRELGIRLVEGLGPA"
BASE COUNT          330 a          789 c          803 g          326 t
ORIGIN      
        1 tgcccgcggg gccgatcgcg atgaccagat cgtgcccgcg caccgcctcc aggtagcgct
       61 tctggttcgc ggtcttcggg gtgatgaagc ggcgtcgcga cggcaccgcg atggcgtcgg
      121 ccagcaccga tttgacgtcg gcgcccggct cgtgccccgc gatgcgcagg gccgagcgga
      181 tgtcggcggc gccgagcgcg ggccgctccc gcagcatggc caccagctcc cgcaccaccc
      241 gctccgcctc cagcacttgc cgctcttctc cacgaaccgt caggtcgtgg ccgcgcgcgg
      301 tgatgcggac gtcgtactgg gcttcgagct ggcgcaggtg ctcgtcgtgg cggccgagaa
      361 gggcgaggtg gttgagatcc ggaggcagga cgatccgtcg ggtgacgccg gtggcttcag
      421 tcaacgaagg ctcattcctc tgcctgggag catatcacgc gggtccgagg ccttccacga
      481 ggcggatccc gagctcgcgg agctgcttgg ggtccaccgg cgcgggcgcg tcggtgagcg
      541 ggcaggtgcc cttctgggtc ttggggaagg cgatgacctc ccggatggac tcctgccccg
      601 ccatgttggc ggcgaggcgg tccaggccga aggcgatccc gcccatgggc ggcgcgccga
      661 actcgagcgc ctccaggagg aagccgaacc gcgcccgcgc ctcgtccttg gagatgccga
      721 tcaggccgaa cacgcgctcc tggacggtgg gctggtggat gcggatggag ccgcccgccg
      781 cttcctggcc gttcagcacg agatcgtagg ccttggcgcg ggcgcgaccc ggatcggatt
      841 cgaggagcgg gaggtcttca tcacggggcg cggtgaacgg atggtggacc gcgtcccacc
      901 gcttctcgtc gggattccac tcgaccaggg gaaaatcgat gatccaagca aagacatcgc
      961 ggtcggtcgg gatcaggttg aaccggcgcg ccatgtccac gcgcagccgc cccagcacgg
     1021 tggccgcgac cgcgggcgtg tcggccacca gcagcaacag gtcgcccgcg acgccgccca
     1081 gcgtgtcgag gagccgcgcc tggatgccct ggatgaactt ggccaccggg gactgcagcc
     1141 cgtccgggtt caccttgatc cagaccagcc ccttggcgcc ggcctgcttc gcctccgcgg
     1201 tgagatcgtc gagctccttg cggctcatgc cgcccgcggc gggcacgcgg agccccttca
     1261 ccgcgccccc cgcggccacc gtctgggcaa aggcctggaa ctcgccgccc gcgaacaggg
     1321 ccgagcagtc ctggagctcc aacccgaagc gcagatcggg cttgtcggag ccgtagcggc
     1381 ccatcgcctc gtcgtacggg atacgcggga acggccgcgc gagctcgacg cccttcacgc
     1441 gccgccacat ctcggccacc aggccctcga tgatgggcag gaagtcgtca cggtcgagga
     1501 acgacgtctc gatgtcgatc tgggtgaact cgggctggcg gtccttccgg agatcctcgt
     1561 cgcggaagca gcgcacgatc tggaagtacc gctcgaagcc cgccaccatc aggagctgct
     1621 tgaagagctg cggggactgg ggcagcgcgt agaagctgcc cggctgcagg cggctcggca
     1681 ccaggaagtc gcgcgcgccc tccggggtcg agcgcgtcag gaacggcgtc tccacctcga
     1741 ggaacgcctc gccgtggagg tagtcccgcg cggcgcggca gatctggtcc cggatctgaa
     1801 aggcccgcag cacgtggggg cggcgcatgt cgagataccg gtacttgagc cggagggtct
     1861 cgtcgatctc cgcgtcgtcg tcgagctgaa agggcaacgg ccgcgattcg ttgaggatgc
     1921 gcacctcgga gacctggacc tcgacgccac cggtggccag cttcgggttc tcggtgccgg
     1981 gcgggcgcgc ggacacggtc ccgcgcaccg ccagcacgaa ctcggtccgg accgcctccg
     2041 ccctggcgtg cgtcccgccg ccggcgcccg ggttgaacac gcactgggtc aggccttccc
     2101 ggtcgcgcag gtcgatgaaa aggaggccgc cgtggtcccg gcggcgatac gcccatccca
     2161 tcagcgtgac cacctgcccg acgtgcaggc tccgcagcgc gccgcagcta tgggtccgcc
     2221 gccagccgcc caggctctcg gtcacgcg
//
LOCUS       DASUFA010000023        21172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1035807, whole genome shotgun sequence.
ACCESSION   DASUFA010000023 DASUFA010000000
VERSION     DASUFA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 21172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21172
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035807"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1337
                     /locus_tag="VFX87_00775"
     CDS             75..1337
                     /locus_tag="VFX87_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4993462.1"
                     /translation="MKDNLRFVDSDMHIMEPPDLFERYLDPKFRDRVILPIGADGRPK
                     RGTIVIDGLPTTMDAEMQQYRKRGRGGAASAHSTQPLSGSRLQGTNRLDFAIERNYDP
                     EAQVMGMAIEGVDIAVLYPTTGLSLLARDGMDPQLSLALCQAYNNWIHEFCRYSPERL
                     KFVAMLPVHDVHLAGRELHRCVRELGAVGSFIRPNLVNGHYWHSNYWDPLYSLHEELD
                     VTWGFHEGTGAWNSHMNVLYGENRFYRHVASHWIEMQQALIAMIIGGVFEFHPKLRVG
                     FLEAQNSWVPGILSRIEWDYPQYHDTHAPYLSLTPKEYFRRNCWAAVEGSEPEIEATA
                     GLIGADRMCVSTDYPHFDSNFPNVSENVLKSCSRETAAQILMGGAHLYGFSETDFAKA
                     DAAAAARAGTNGGPAAASGKRRGAVVGG"
     gene            complement(1348..2424)
                     /locus_tag="VFX87_00780"
     CDS             complement(1348..2424)
                     /locus_tag="VFX87_00780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993463.1"
                     /translation="MDFGLFFLMQRDEEWSERAVYDSGLEQMLAAEALGYSSVWIAEH
                     HFNDYGLCPVPPVLAAFLAARTRALRLGMGVSLLPLHHPVDLAEQLAVLDVVSGDRLD
                     VGIGRGGTLQDYQTFQSDREDSRARVEEGIALIRKSWSGEPFDFAGRFHTAERLHVRP
                     RPVQRPHPPLYIAANSEDSVHSAARLGLPTLSSFFVPIEELQRRHRLYREESLAIGRA
                     EAEVAGLERQAWGMRVVHVAPDHAEALRAIEAPFMGYQRKMSVLRSDSTGGTVPGSFN
                     RSLLRLRTFREYLDDGWALIGTPGEVREGLQKYLDATGYQHVLLLMALPGLDTSLALR
                     SMRLFAEQVAPAMVPANGRVAAGP"
     gene            2560..4251
                     /locus_tag="VFX87_00785"
     CDS             2560..4251
                     /locus_tag="VFX87_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993464.1"
                     /translation="MPFDRLGQRSRLNRRQFLMRAGWTGLGASALVAGPGRRRALAQA
                     GTKYPDWIPASPKPPKRGGVLTRASAWDPPVMDPRLTQSVGLYQFAGLVGSRLVRYAF
                     PDEVTGPSDMSLKGDLAESWTSSPDARVWTFKLRQGVKWHNVAPLNGREFVAADVKYC
                     LEAYAKEGAQSFTFQEVEAIETPDKHTVRIHLKTPNVLFPQNVAEPITVMFSREVLEE
                     DGDLKKRMIGTGPFLLKEHSRKVRVVLTRNPDYWEKGRPYVDEYVILSTPDAATRIAA
                     FRTGQSDFIWLASPSEVETVRKTNANVQVHSYHNTLTPFGIALSQDKPPFNDVRVRRA
                     LSMAIDRQKQVDTIFEGHGILGWGVPYIYHQDKPLTAASFGPWWQYRPAEAKKLLAEA
                     GHPNGFDTTLFYYEYFPQMTSQVQVVQQDLRRNLNINVKITKLDYTTYYGRYVESKWD
                     GMAWGFQSGHAIGLDERTYQYMHSKSTKNFFRVADPVIDELTTKLRQTAAPADQRVLT
                     KKIVDREFDQVLRMWMPYDNGFLVFQPHMRNGASAALRRTDGYGSSTISRVWLDK"
     gene            4276..5226
                     /locus_tag="VFX87_00790"
     CDS             4276..5226
                     /locus_tag="VFX87_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4993465.1"
                     /translation="MYKYTLRRFMLAVPVLLLSSLIVFGLMRVMPGDALTALMGESGN
                     IGEKELQKIRHDLRLDLPYWEQYLLWLWQMVSLRPGDSIFTGEPITTSLAKAIPVTLE
                     LAALAMILGLVVAIPIGVLSATRQDTAPDYVGRVVAVSGLSLPDFWLGTLVITFAAIW
                     FHWIPAIGYVSFWESPVRNLQQFLLPAAVLGYRLSAATMRMTRSTVLEVLREDYVRTA
                     WAKGLEGRIVVYKHALKNALIPVVTIVGGQLGVLLAGTVIVETIFALPGMGRLTVEAI
                     LFRDYPVVQTNVMLVAGTLVSLNLLVDLTYAWLDPRIRYQ"
     gene            5240..6136
                     /locus_tag="VFX87_00795"
     CDS             5240..6136
                     /locus_tag="VFX87_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4993466.1"
                     /translation="MAVTAPAAHFEAEPTVELGRARALWTVMRRKPLGAVSAALIVVI
                     VLTAIFADVLAPYDPLAAQPEIRLAAPSWEHPFGTDDIGRDVFSRVIYGSRISLWVGL
                     LAVGIGTVAGMIIGLLCGYCEGRTDLVFQRIMDAIQAIPGLVLALAIVSVLTPNTTNA
                     MLAIAMVIIPGNSRIVRGAVLAAKQNRYVEAAQAIGCRHPRIILTHILPNVTAPILVI
                     ASIWLGNAILIEASLSFLGVGTQPPTPSWGLMLSSSGRAFMEQAPWLALFPGLAISLA
                     VLGFNLFGDTLRDAWDPKLRRG"
     gene            6141..7670
                     /locus_tag="VFX87_00800"
     CDS             6141..7670
                     /locus_tag="VFX87_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993467.1"
                     /translation="MRALVSVILAVVLPLGAGLTPAAAQAKPEGEMRWALYVTLAPAW
                     FDPAEVVGVLTPFWVLNAMHDALVKPMPGNHQTPSLAESWKVSPDGLVYEFKLREGLK
                     FHNGDPFTAEDVRWSFHRAKGAKVLQEKVRDVEIAGPYRVRFHLHEPWPDFMTFYGTY
                     ATGAAWIAPKKYMEQVGPDGFKKNPVGLGPYKFVSHTPGVELVMEAYEGYWRKMPSVK
                     RLVYKSVPEATTRLAMLKRGEVDLAYLLDAPQAEEVKRDPSLKLAFSGGIGTFYLDYL
                     DQWDPKSPWHDRRVRLAASHALDRRALSDAETLGASRPNGSLIPRTFQFALPLDPHPY
                     DPAKAKQLLAEAGYPNGFDAGELYPWPPYTAFSEAVGGYLGAVGIRVRLRTMERAAFY
                     SALASKKLKGLCVCINAVYGNAASRIAQTVPSDGAFAYGGYPDIDALYKQQSREIDPR
                     KREAQLHQIQRLLHERVRFAPIMDYIWPSGVGPRVAEPALMLIDPYPWSAPLEEVRLK
                     K"
     gene            complement(7625..8005)
                     /locus_tag="VFX87_00805"
     CDS             complement(7625..8005)
                     /locus_tag="VFX87_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX4993468.1"
                     /translation="MRTVAILLTGLVALEHVGIMLLEMFFWDHPVGQKIFNITPEVSA
                     VSRVLAANQGLYNGFLAAGLFWGLGRGDRRITVFFLACVAIAGIFGGLTAKTSILATQ
                     FLPAALALAAYFLSRTSSSGADHG"
     gene            complement(8021..8500)
                     /locus_tag="VFX87_00810"
     CDS             complement(8021..8500)
                     /locus_tag="VFX87_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4993469.1"
                     /translation="MGARAESLAKQFEAKAAEMTAVVAKLSDADWKKITAAEKWPVGV
                     TAHHLARGHEAILGIVKTIAAGKSIPNFTTTMLDEMNAAHAREHANCTKAETIALHEK
                     GAAVAAAGVRALSDAELDRSGTVLTGMPAMTCQQVVEGILINHMNDHLGSIRKTVGA"
     gene            8530..8706
                     /locus_tag="VFX87_00815"
     CDS             8530..8706
                     /locus_tag="VFX87_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993470.1"
                     /translation="MKNVEMTVAGNILTIKVDLTKEFGPSASGKTIIIASTEGNITIP
                     EREEKVGLNVYRKK"
     gene            complement(8651..9967)
                     /locus_tag="VFX87_00820"
     CDS             complement(8651..9967)
                     /locus_tag="VFX87_00820"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX4993471.1"
                     /translation="MTHPVFPLRAVTALFLERQHLIRPRIHRLTPARLTRFVEDVGGL
                     QMDSINVLDRAHYLTVWSRFGPYDRPRLDRLVYRRRLFLEYWAHAACLVPASMLPWWR
                     RAMLDYRRRHTGWSNWLRRNTKLLGLVTETIRANGPMSHGDLEESRPRRGSSGWWNWR
                     PVQHAIHYLWMTGVLTVHSRRHFQKRYDLLERAVAASAAEPVSSEQFLRWHVERSLHA
                     MGAATELDLSRYLTYPRLAPAGRRAALRAMVAEGLVTEIAVKGRAGRWLVLTRDLPAL
                     RRAGRRRTVSDGTTLLAPFDSLLWHRDRVSRLFGFDYRIEVYTPGPKRVHGYYTLPIL
                     HHGHLIGRLDAKAHRVARWLEVRHVHFERWLARSEAPPAGWGEVDRDQMVTELADTLA
                     SLALFVGADRITLGRVTPGRLRGPLRRALTSSGTRSAPPFPPAPES"
     gene            10049..10468
                     /locus_tag="VFX87_00825"
     CDS             10049..10468
                     /locus_tag="VFX87_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993472.1"
                     /translation="MKRLMAGCLVLAVLLTITLAPGDALARGRFHRGGGHIGFWGPGL
                     FLGGLALGAAVTAPYRYGYPYYYGAYGPDYYYAPPPTVIYQQAPPAYTAAPPPVQREV
                     VYAHGKYVLYGDGVTQAWQWVWVPNAPASPPPPPPAR"
     gene            10505..11254
                     /locus_tag="VFX87_00830"
     CDS             10505..11254
                     /locus_tag="VFX87_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993473.1"
                     /translation="MRLGGRIALVTGAQQGIGRAIAIALGRDGANVCVNFLDDRGAAE
                     RVAAEIRGHGGQAMTVAGDVARSQDVAAILAAAIAELGTPDILVNNAGVFPRASFLDL
                     AESEWDHVLGVNLKGGFLCAQAAARAMVAAGKPGAIINISSSAIRGDARGVHYSASKA
                     GVLGMTRAMALALAPHRIRVNAIAPGLTDTQQPRYGNTEEQIAARAQEIPLGRMAQPE
                     EIARVAVFLASEDAAFITGELIHANGGAYLA"
     gene            11527..11937
                     /locus_tag="VFX87_00835"
     CDS             11527..11937
                     /locus_tag="VFX87_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993474.1"
                     /translation="MKDITVIRLEDTERVSFGPLSHYQPIVGDDSGSTPVRTGIQTAQ
                     PGYAAAVHSHPYIEILHILDGVAEAWIDGKPESMVVLRKGDTIAVPPETPHAFRVVGD
                     QVLRLLGTHVSPKRIVNYKDGVTSDARGYRSEVS"
     gene            11941..12759
                     /locus_tag="VFX87_00840"
     CDS             11941..12759
                     /locus_tag="VFX87_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993475.1"
                     /translation="MRLGVGVATMNPDGSPLTGDVLVGSARMAERIGFDSLWFFDAMS
                     RGFILPDPLIGASVAAAVTTRAEVGTCVLQVPLRNPVELAHRILTAHLVSAGRLLLGV
                     GAGSTKADFDALGLDYAARLQAFEDALPVMRRLWRGEKVGAANLTPWPAALGGPAVLI
                     GSWGGKRWIPRAAQEFDGWIASAGRTNLNTLAEGIQRYRAAGGKRAIATNISVDLTGP
                     TDPCPDDGPFHLRCAPDVAAQRLRRLHDLGFDDAVLVLKSASEANLAAARALLK"
     gene            12767..13969
                     /locus_tag="VFX87_00845"
     CDS             12767..13969
                     /locus_tag="VFX87_00845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4993476.1"
                     /translation="MRQTAEVVVVGGGVNGASIAYALAAKGVKVVLVEKGALAGGASG
                     RSSALVRMHYTNEWDARLAWASFPVFRNWAELMGGPPVFTRTGFVNVVAPRYAQHLRQ
                     NVEMLRRIGVDTTAITPLELRELQPFAHVDDVGAAAYEPESGYANPAETVEGFRRRAV
                     ELGARVFPWIAVTRIVRRESRVTGVETAAGRIDAGHVVVAAGAWSRRLCEEIGLAVPA
                     RPKAIDTVAVSRPAELRDPHMVFIDNVQGNYFRPESGGLTLVGVPCQEWDIDPDTLGT
                     GLPPEAAGLGAQLLTHRIPAMERATLSRGYRAFDGYSPDRHAILGAVDGIDGLYLATA
                     FSGSGFKIAPAVGQCMAELITDGRAKTVDIEVFRLGRFAQGKTVEGPYPYAVRPDYVD
                     PASAGGAQ"
     gene            13966..14679
                     /locus_tag="VFX87_00850"
     CDS             13966..14679
                     /locus_tag="VFX87_00850"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX4993477.1"
                     /translation="MSAGRPPGVEFHTFTALGRCPRTGRLGIATATRSLAVGARVPHV
                     RAGLGAIAIMAIADARLGYMALRLLELGYKAPAVIEELVKGDPYAEYRQLGVIDADGF
                     AAARTGKMNRDWAGHHVRDDHIVLGNVLTGPHVLDAMEKAFAADPAEDLEERLLRAIE
                     AGRDAGGQHGGQQSAALLVYDDRPFAHVDLRVDVHVEPIGELRRVFDVYRPAIPYYNL
                     RQVDARVPPLQDWLAARPR"
     gene            complement(14622..17048)
                     /locus_tag="VFX87_00855"
     CDS             complement(14622..17048)
                     /locus_tag="VFX87_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4993478.1"
                     /translation="MTTTGASFLTARTRSAPQGFGRPVPRKEDARLVTGRGRFSDDFN
                     LPGQAHAHLVRSPHAHARILSIEAAAALAVPGVLAVLTGRDAVADGLASIPHKPVPTN
                     PNEFPLGGREGGRVLVTPHPPLAADVARFAGEAVAMVIAETAAVAADAAERVQVAYEP
                     LPSVTATPAAGEPGAPVLWSEFGSNLCVDSVAGDARAADAAFAAAAHTVRLDTWVPRI
                     TGVPMEPRAALGVHDAATGRYTLYAGSGGVVRQKADLAEVLGVPEAAVRVVAEDVGGN
                     FGTRNSFYPEFALVAWAARRLGRPVKWTCERREAFLTDYQARDLVSSMELALDADGRF
                     LALRGVNTSNVGAHTVTFVPLNKGRELATTVYRVPAAAVRGRAVLSNTSPLSAYRSAG
                     RPQVMFVMERLIDLAARRHGFDRLALRRRNLVPSAAMPYTNPFGIVYDSGDYVAVQDR
                     AVALADWGGFEARRREARARGRYRGIGLANYIEIATGAPRERAHITVQPEGRIDVVIG
                     TLAAGQGHETSFAQLVVEWLGGALEQVRLISGDTDRVAVGGGSHSGRSMRLGGVVMAK
                     AADQIVEKGARLAAWRLESAVADLEFAGGRFRVRGTDRAISLFEVAAAAGRPDAPEDL
                     RGPLDGAGDETVSTPSFPYGCAVCEVEVDPETGVVEVVRYTSVDDVGRAINPLIVDGQ
                     THGGIVQGLGQALWEHCRYDAESGQLQSATFMEYAMPRADCLPSFATEISEVPSTANP
                     LGLRGGGEGGTTPALGAAVNAIVDALAELGVEHLEMPVTPERVWGAIRDAQRGRAASQ
                     SWSGGTRAST"
     gene            complement(17100..17435)
                     /locus_tag="VFX87_00860"
     CDS             complement(17100..17435)
                     /locus_tag="VFX87_00860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4993479.1"
                     /translation="MEIMTAPFARTMTSTASSHHQRTRRINRRASVSWTVFARVRGQR
                     CRFHTVDVSTRGAKLRPKGDLLPGTSLTLQFVKPNGRRVRVAAMVWRVDADGLGVFFL
                     GSESEGLMG"
     gene            complement(17605..18438)
                     /locus_tag="VFX87_00865"
     CDS             complement(17605..18438)
                     /locus_tag="VFX87_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX4993480.1"
                     /translation="MRVGLSVDPPLAAIPALLREEGVDVYQTVLRDPGRFGNYGVPEP
                     ADRAALVEGLRGRRVWGVAHGSLLVNLASPEGRIRNSSVSSLLADLKVAAEVGLDAVC
                     FHVGYAKGHPSADAALILATRKLGELIGRMPAGPRLLIENSCEGSELGQTIAELARLL
                     RDVGAPPEQLGLLIDTCHLHAAGFDLAGSDSGQRLADALAAEGVLERVVAFHLNDSQL
                     ACGAHRDRHAIPGAGTIGLGVVSVASHPAFAALPGVIELSVEDARRGLAFLRERGATG
                     G"
     gene            18512..19171
                     /locus_tag="VFX87_00870"
     CDS             18512..19171
                     /locus_tag="VFX87_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993481.1"
                     /translation="MRTAGVVALMMLTMAGVVASWADRWPAPQTREVWSESRDYFVRV
                     IPGTSIGETVGFRGAPTGRQATAELYRRASDRSYRLAWTVSLLNPVAPIDLFLTDRGY
                     LVTLDNWHNMGYGKIVAFYAPDGKLVRSHALDDLFSAAEIDKLPGSVSSIWWRKQIAY
                     VRLDQQSLYVSVGDGGREFVFETETGAYQFCETGDGAYRCRSSNDQRQWRGYVDPPSR
                     R"
     gene            complement(19187..20185)
                     /locus_tag="VFX87_00875"
     CDS             complement(19187..20185)
                     /locus_tag="VFX87_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993482.1"
                     /translation="MRFGFFFWPYTPEYTTRMARRGEELGFDLVGIADTPGNALDPWV
                     AMTLAAAATSRVMLATCVTNLVTRHPSITASAAASVDVAAGGRTILGVGAGHSGVANV
                     GGAPSRASDLRDGLTFLKTALSGAPASWRGAPTHLSWIQRAVPVYAAASGPAALRAAG
                     AAADGAFVNYGLGPDHVSRVRGLIAEGAAGTRRPASAIDVWSIACLDVAERREDALEK
                     LGNILGFVAAYILGPDPAGRGVPPDLVPAVHELRATYTTRQRDMDPALTRRLGLFDYL
                     RARLAIAGTPEDCVAQVRAAEAAGVTQLMFTVSLAADPERTVELFGKAVLPRLARA"
     gene            complement(20197..>21172)
                     /locus_tag="VFX87_00880"
     CDS             complement(20197..>21172)
                     /locus_tag="VFX87_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993483.1"
                     /translation="RKLVDHDKIHVMTGILLVNVGNSLVPLIERDRLPTLFLTTPDDL
                     TKRKVAKWILRSNFSASQIMHPLGDYAARTLKYRRVATIAMDNGFGHEEAGGFQRVFE
                     DAGGKVVQKIWVPLNALDFAPYLAQVQKDVDAVCAVFVAGQAVRFVKQYGESALKGKT
                     PLIGTGVMIDESALRGMGEEAVGVVGSLLWSPTLQNPANQTFMKMAEAKLGRTPAYFH
                     AIMYSSGRWITEAANALGGQVEDREKLVAAIRRSIESTPDPRGPIKLDDWGNPTENVY
                     ILRVDKVGARLATTVIHTYPAVSQFWTYKPEEFLKSPPYTRDYPPAKP"
BASE COUNT         3156 a         7415 c         7534 g         3067 t
ORIGIN      
        1 atcgagggcc ggaacatgga caaggtcaag cggctggcct acgacaaggt ctgagagaac
       61 aggaggcccg agccatgaaa gacaacctcc gcttcgtcga ttccgacatg cacatcatgg
      121 agccccccga tctcttcgag cggtacctgg atccgaagtt ccgcgatcgg gtgatcctcc
      181 ccatcggggc cgacggccgg ccgaagcgcg gcaccatcgt catcgacggg ctgcccacca
      241 ccatggacgc ggagatgcag cagtaccgca agcgcggccg tggtggggcc gcgagcgcgc
      301 acagcaccca gccgctctcg ggctcgcggc tgcaggggac gaaccggctc gacttcgcga
      361 tcgagcgcaa ctacgacccc gaggcgcagg tgatggggat ggcgatcgag ggcgtggaca
      421 tcgcggtcct ctacccgacc accggcctga gtctgctcgc ccgcgacggc atggacccgc
      481 agctctcgct cgccctctgc caggcctaca acaactggat ccacgagttc tgccggtaca
      541 gccccgagcg gctcaagttc gtggccatgc tgcccgtcca cgacgtgcac ctggccggcc
      601 gggagctgca ccggtgcgtg cgcgagctgg gcgcggtcgg ctccttcatc cggccgaatc
      661 tggtcaacgg ccactactgg cactcgaact actgggatcc gctctacagc ctccacgagg
      721 agctcgacgt cacctggggc ttccacgagg ggaccggcgc ctggaactcc cacatgaacg
      781 tgctctacgg cgagaatcgg ttctaccgcc acgtggccag tcactggatc gagatgcagc
      841 aggcgctcat cgccatgatc atcggcggag tgttcgagtt ccatcccaag ctgcgcgtcg
      901 gcttcctgga ggcgcagaac tcgtgggtgc cggggatcct gtcgcggatc gagtgggact
      961 acccgcagta ccacgacacc cacgcaccct acctgtccct gaccccgaag gagtacttcc
     1021 ggcggaactg ctgggccgcg gtggagggca gcgagcccga gatcgaggcg accgcgggcc
     1081 tgatcggcgc cgaccggatg tgcgtgtcca ccgactaccc gcacttcgac tcgaacttcc
     1141 cgaacgtgtc ggagaacgtg ctgaaaagct gctcgcgcga gaccgccgcg cagatcctga
     1201 tgggcggggc gcatctctac gggttcagcg agaccgactt cgcgaaggcc gacgcggcgg
     1261 cggcggcgcg cgcgggcacc aacggggggc cggccgccgc gtccgggaag cggcgcgggg
     1321 cggtggtcgg cggctaggcg cggcgggtca cgggccggcc gcgacgcgcc cgttcgcggg
     1381 caccatcgcg ggcgccacct gctcggcgaa caggcgcatc gagcgcaggg ccaggctcgt
     1441 gtccaggccc ggcagcgcca tcagcagcag tacgtgctgg tagccggtgg cgtccagata
     1501 cttctgcagg ccctcgcgga cctcgcccgg cgtgccgatc agggcccagc cgtcgtcgag
     1561 gtattcccgg aaggtgcgca ggcgcagcag tgagcggttg aacgagccgg gcacggtgcc
     1621 gccggtggag tcggagcgca ggaccgacat cttccgctgg tagcccatga acggggcctc
     1681 gatggcccgc agcgcctccg cgtgatcggg cgcgacgtgc accacgcgca tcccccacgc
     1741 ctggcgctcc agccccgcca cctcggcctc cgcccgcccg atcgccagcg actcctcgcg
     1801 gtagagccga tgccggcgct gcagctcctc gatcggcacg aagaacgagg agagcgtggg
     1861 caggcccagg cgcgccgccg agtgcacgct gtcctcgctg ttggccgcga tgtagagcgg
     1921 cgggtgcggc cgctgcaccg ggcgcgggcg gacgtgcagg cgctcggcgg tgtggaagcg
     1981 gcccgcgaag tcgaacggct ccccgctcca cgacttccgg atcagcgcga tgccttcctc
     2041 gacgcgcgcc cggctatcct cgcggtcgga ctggaaggtc tggtagtcct ggagcgtgcc
     2101 gccgcggccg atgcccacgt cgagccggtc gccgctcacc acgtccagca ccgcgagctg
     2161 ctcggccagg tccaccgggt gatggagcgg cagcaggctc acccccatgc cgagccgcag
     2221 ggcgcgcgtg cgggcggcca ggaacgcggc gagcaccggc gggaccgggc agagaccgta
     2281 gtcgttgaag tggtgctcgg cgatccagac cgacgagtac ccgagtgcct cggcggccag
     2341 catctgctcg aggcccgagt cgtagaccgc ccgctcgctc cactcctcgt cgcgctgcat
     2401 caggaagaac agtccgaagt ccatggccgg tagcatagct tgacttcgac gctcggggtg
     2461 cggcatagtc ccgggcactc ggagccggtg taatgacgca tcggctcgag ttcgctcgaa
     2521 ggtcctttcc ttcacgaaga cgtgagaagg agagccgcga tgcccttcga tcgcctcggt
     2581 cagcgaagcc ggctcaaccg ccgtcaattc ctcatgcgtg ctgggtggac cggcctgggc
     2641 gccagcgccc tcgtcgcggg ccctggccga cgccgcgccc tcgcccaggc ggggaccaag
     2701 tatcccgact ggatcccggc cagccccaag ccgcccaagc gcggcggggt gctgacccgt
     2761 gcctccgcgt gggatccgcc ggtgatggat ccgcggctga cccagtcggt cggcctgtac
     2821 cagttcgcgg gcctggtcgg tagccggctc gtccgctacg cgtttccgga cgaggtcacc
     2881 ggcccctccg acatgagcct caagggcgac ctcgcggagt cgtggacgtc gagcccggac
     2941 gcccgcgtgt ggaccttcaa gctgcgccag ggcgtgaagt ggcacaacgt ggcgccgctc
     3001 aacgggcgcg agttcgtggc cgccgacgtc aagtactgcc tcgaggccta cgcgaaggag
     3061 ggggcgcagt ccttcacctt ccaggaggtc gaggccatcg agacgcccga caagcacacc
     3121 gtccgcattc acctcaagac gccgaacgtg ctcttcccgc agaacgtggc ggagccgatc
     3181 acggtgatgt tctcccgcga ggtgctggag gaggacggtg atctcaagaa gcgcatgatc
     3241 gggaccggcc cgttcctcct gaaggagcac agccgcaagg tccgggttgt gctcacgcgc
     3301 aatccggact actgggagaa gggccgtccg tacgtggacg agtacgtgat cctctcgacc
     3361 cccgacgcgg cgacccgcat cgccgcgttc cgcaccgggc agagcgactt catctggctg
     3421 gcgagcccct ccgaggtcga gaccgttcgg aagacgaacg ccaacgtgca ggtgcactcc
     3481 taccacaaca cgctgacgcc gtttgggatc gcgctgtccc aggacaagcc gcctttcaac
     3541 gacgtgcgcg tgcgccgcgc gctctcgatg gcgatcgacc ggcagaagca ggtggacacc
     3601 atcttcgagg ggcacggcat cctcggctgg ggcgtaccct acatctacca ccaggacaag
     3661 ccgctcaccg cggccagctt cggcccgtgg tggcaatatc gccccgcgga ggccaagaag
     3721 ctgctggccg aggccgggca cccgaacggc ttcgacacca ccctgttcta ctacgagtac
     3781 ttcccgcaga tgacctcgca ggtgcaggtc gtgcagcagg atctcaggcg caacctcaac
     3841 atcaacgtca agatcaccaa gctcgactac acgacctact acgggcggta cgtcgagagc
     3901 aagtgggacg gcatggcgtg gggcttccag tcggggcacg cgatcggtct cgacgagcgc
     3961 acctaccagt acatgcactc caagtccacg aagaacttct tccgcgtcgc cgatccggtc
     4021 atcgatgagc tgaccaccaa gctgcgccag acggcggcgc cggccgatca gcgggtgctc
     4081 accaagaaga tcgtggatcg ggagttcgac caggtgctgc ggatgtggat gccctacgac
     4141 aacgggtttc tggtcttcca gccgcacatg cggaacgggg cctcggccgc cctccggcgc
     4201 accgacggct acggctcctc caccatctcg cgagtctggc tggacaagta ggaggcgcgg
     4261 gagaggccgc gcgcgatgta caagtacacg ctccgccggt tcatgctggc ggtcccggtg
     4321 ctcttgctgt cctcgctgat cgtcttcggt ctgatgcgcg tcatgccggg ggacgcgctc
     4381 accgccctca tgggcgagtc cggcaatatc ggcgagaagg agctgcagaa gatccgccac
     4441 gacctccggc tcgacctgcc gtactgggag cagtacctcc tctggctctg gcagatggtg
     4501 agcctccgcc ccggcgactc catcttcacc ggcgagccca tcaccaccag cctggccaag
     4561 gcgatcccgg tgaccctcga gctcgccgcg ctcgcgatga tcctcgggct ggtggtcgcc
     4621 attcccatcg gcgtgctgtc ggccacgcgg caggacaccg cgccggacta cgtggggcgg
     4681 gtggtggcgg tgtcggggct gtcgctgccc gacttctggc tgggcacgct cgtcatcacc
     4741 ttcgcggcga tctggttcca ctggatcccc gcgatcggct acgtgagctt ctgggagtcc
     4801 cccgtccgca atctccagca gttcctgctg cccgccgcgg tgctcggcta ccggctctcc
     4861 gccgcgacga tgcgaatgac gcgctcgacc gtgctcgaag ttcttcgaga ggactacgtg
     4921 cgcaccgcct gggccaaggg gctcgagggc cgcatcgtgg tctacaagca cgcgctgaag
     4981 aacgcgctga tcccggtggt gaccatcgtg ggcggtcagc tcggggtgct cctggccggg
     5041 accgtgatcg tggagacgat cttcgcgctg cccggcatgg gccggctcac cgtggaggcg
     5101 atcctcttcc gtgactaccc ggtggtgcag accaacgtga tgctggtggc ggggaccctc
     5161 gtgagcttga acctgctcgt ggatctcacc tacgcgtggc tcgacccgcg catccgctac
     5221 cagtgaacgg ggacccgcga tggccgtgac cgcacccgcc gcccacttcg aggccgagcc
     5281 caccgtcgag ctgggtcgcg cgcgggcgct ctggacggtg atgcggcgca agccgctcgg
     5341 ggccgtctcc gcggccctca ttgtggtgat cgtgctcacc gcgatcttcg ccgacgtgct
     5401 cgcgccctac gacccgctcg cggcccagcc ggagatccgg ctggcggctc caagctggga
     5461 gcaccccttc ggcaccgacg acatcggacg cgacgtcttc agccgcgtca tctacggcag
     5521 ccgcatctcg ctctgggtcg gcctgctcgc ggtcgggatc gggacggtcg cgggcatgat
     5581 catcgggctc ctgtgcggct actgcgaggg gcgcaccgat ctggtgttcc agcgcatcat
     5641 ggacgccatc caggcgatcc ccgggctggt gctggcgctg gccatcgtct cggtgctcac
     5701 gcccaacacc accaacgcga tgctcgcgat cgcgatggtg atcatccctg gcaactcccg
     5761 catcgtgcgc ggtgcggtgc tcgccgccaa gcagaaccgc tacgtggagg ccgcgcaggc
     5821 catcggctgc cgtcatccgc ggatcattct gacccatatc ctgcccaacg tgaccgcgcc
     5881 catcctggtc atcgcctcca tctggctggg caacgccatc ctgatcgagg cgtccctctc
     5941 atttctaggc gtgggcacgc agccgccgac gccgtcgtgg gggctgatgc tcagcagtag
     6001 tgggcgcgcg ttcatggagc aggcgccgtg gctcgcgctc ttccccggcc tcgcgatcag
     6061 cctggccgtc ctggggttca atctcttcgg cgacacgctg cgggacgcgt gggatccgaa
     6121 gcttcggcga ggctagaccc atgcgcgctc tcgtgtccgt gatcctggcg gtcgtcctgc
     6181 cgctcggtgc cggtctcacc cccgcggccg ctcaggccaa gccggaaggg gagatgcgct
     6241 gggcactcta cgtgacgctg gcccccgcct ggttcgatcc ggccgaggtc gtcggggtgc
     6301 tgaccccgtt ctgggtactc aatgcgatgc acgacgcgct cgtgaagccg atgcccggca
     6361 accaccagac gccgagcctc gccgagtcct ggaaggtgag cccggacggg ctcgtgtatg
     6421 agttcaagct gcgcgagggc ctgaagttcc acaacggcga cccgttcacc gccgaggacg
     6481 tgcggtggag cttccatcgc gccaagggcg ccaaggttct ccaggagaag gtgcgcgacg
     6541 tcgagatcgc ggggccctac cgggtgcgct tccacctcca cgagccgtgg ccggacttca
     6601 tgaccttcta cgggacgtac gcgaccggcg ccgcctggat cgcccccaag aagtacatgg
     6661 agcaggtggg cccggacggt ttcaagaaga acccggtcgg cctcgggccc tacaagttcg
     6721 tcagccatac ccccggcgtc gagctggtga tggaggccta cgagggctac tggcgcaaga
     6781 tgccctcggt caagcgactg gtctacaaga gcgtgccgga ggccaccacg cgtctggcca
     6841 tgctgaagcg cggcgaggtg gatctggcct acctgctcga cgccccccag gccgaggagg
     6901 tcaagcgcga tccctcgctc aagctcgcgt tctcgggcgg catcggaacg ttctacctcg
     6961 actatctcga tcagtgggat cccaagtcgc cgtggcacga tcggcgggtg cggctggccg
     7021 ccagccacgc gctcgatcgc cgggcgctca gcgacgcgga gacgctcggc gcctcccggc
     7081 ccaacggcag cctgatcccg cggacgttcc agttcgcgct cccgctcgac ccgcatccct
     7141 acgatcccgc gaaggcgaag cagctgctgg ccgaggccgg gtatcccaac ggcttcgacg
     7201 cgggggagct ctatccgtgg cccccgtaca ccgccttcag cgaggcggtc ggcggctacc
     7261 tgggcgcggt ggggatccgg gtgcgcctgc gcaccatgga gcgggccgcc ttctactccg
     7321 cgctcgcgtc gaagaagctg aaggggctct gtgtctgcat caacgcggtg tacggcaacg
     7381 cggcctcgcg catcgcccag accgtcccca gcgacggagc cttcgcttac ggcggctatc
     7441 cggacatcga cgcgctctac aagcagcaga gccgcgagat cgatccgcgc aagcgggagg
     7501 cacagctcca ccagatccag cgcctgctcc acgagcgggt gcgcttcgcg ccgatcatgg
     7561 actacatctg gccgagtggc gtgggccccc gggtggccga gcccgcgctg atgctgatcg
     7621 atccctaccc gtggtcggcg ccgctcgaag aggtccggct caagaagtaa gcggccagcg
     7681 cgagcgccgc gggcaggaac tgggtggcga ggatcgacgt cttcgcggtg aggccgccga
     7741 agatgccggc gatcgccacg caggcgagga agaacaccgt gatccgccgg tcgccccgac
     7801 cgagccccca gaagagcccc gccgcgagga agccgttgta gagcccttga tttgcggcaa
     7861 gcacccgtga aacggcggat acttcagggg ttatgttgaa gatcttctga cccaccgggt
     7921 ggtcccagaa gaacatctcg agcagcatga tcccgacgtg ctccagggcc acgagtccgg
     7981 tcagcaggat cgcgacggtg cgcatgccga cggccgaggg tcaggcgccg acggtcttgc
     8041 ggatgctgcc gagatggtcg ttcatgtggt tgatgaggat gccctcgacg acctgctggc
     8101 aggtcatggc gggcatgccg gtgaggacgg tcccgctccg gtcgagctcg gcatcggaga
     8161 gcgcccgcac gccggccgcc gcgaccgccg cgcccttctc gtggagcgcg atcgtctccg
     8221 ccttggtgca attggcgtgc tcgcgtgcgt gggccgcgtt catctcgtcg agcatcgtcg
     8281 tggtaaagtt tggaatcgac ttgccggcgg cgatggtctt cacgataccc aggatcgcct
     8341 cgtggccgcg cgcgaggtga tgcgcggtga cccccacggg ccacttctcg gccgcggtga
     8401 tcttcttcca gtcggcgtcg gagagcttcg caacgacggc ggtcatctcg gcggccttgg
     8461 cttcgaactg tttggccagc gactcggcgc gtgcgcccat ggggcacctc ccagaaagag
     8521 agagatcaga tgaagaacgt ggagatgaca gtagccggaa acatcctgac gatcaaggtg
     8581 gacctcacga aggagttcgg cccgtccgcg tcgggcaaga ccatcatcat cgcgtccacc
     8641 gagggcaaca tcacgattcc ggagcgggag gaaaaggtgg ggctgaacgt gtaccggaag
     8701 aagtgagcgc gcgccggagc gggccgcgca ggcggccggg cgtcacgcgg ccgagggtga
     8761 tccggtccgc gccgacgaag agggccagcg aggccagcgt gtcggccagc tccgtcacca
     8821 tctgatcgcg atccacctca ccccagcccg cggggggcgc ctcgctccgg gcgagccatc
     8881 gctcgaagtg cacgtgccgg acttccagcc accgggcgac gcgatgggcc ttggcgtcca
     8941 gccggccgat gaggtggccg tggtggagga tggggagcgt gtagtagccg tgcacgcgct
     9001 tcgggcccgg ggtgtagacc tcgatccggt agtcgaagcc gaagagccgc gagacgcggt
     9061 cgcggtgcca gagtagcgag tcgaacggcg cgagcagggt ggtgccgtcc gagaccgtcc
     9121 ggcggcggcc cgcgcgacgg agcgcgggca ggtcgcgggt caggacgagc cagcggccgg
     9181 cccggccctt caccgcgatc tcggtcacga gcccctcggc caccatcgcg cgcagcgcgg
     9241 cgcggcggcc ggcgggcgcc aggcgcgggt aggtgagata gcggctcagg tcgagctcgg
     9301 tggccgcgcc catggcgtgg agcgaccgct ccacgtgcca gcggaggaac tgctcggacg
     9361 acaccggctc cgccgcggag gccgcgaccg cgcgctcgag gagatcgtag cgcttctgga
     9421 aatgccggcg cgagtgcacg gtgagcacgc cggtcatcca gagatagtgg atagcgtgct
     9481 ggaccggccg ccagttccac cagccgctac tgccgcggcg agggcggctc tcctccaggt
     9541 cgccgtggct catcgggccg tttgcgcgga tggtttcggt caccaggccg aggagctttg
     9601 tgttgcggcg cagccagttg gaccagccgg tatgccgccg ccggtagtcg agcatcgcgc
     9661 gccgccacca gggcagcatc gaggccggca ccagacaggc cgcgtgggcc cagtactcga
     9721 ggaagagccg gcggcgatag accagccggt cgagccgcgg gcggtcatag gggccgaagc
     9781 ggctccagac cgtgaggtag tgggcgcggt cgaggacgtt gatcgagtcc atctggagcc
     9841 cgcccacgtc ctcgacgaag cgcgtcaggc gcgcgggggt gagccggtgg atgcgcggcc
     9901 ggatcaggtg ctgccgctcc aggaacaggg cggtgaccgc gcggagcggg aagacgggat
     9961 gcgtcatcgt cctggagggt accagcctgg agcgaccgct caaggtgtaa gatgaggttc
    10021 gggtacgatg acgtccagga gggcgggtat gaagcggctc atggcggggt gtctggtgct
    10081 cgcggtcctg ctcacgatca cgctcgcgcc gggcgatgcc ttggcccgcg ggcgcttcca
    10141 tcgcggcggc ggtcacatcg gattctgggg ccccggtctc ttcctcggcg ggctggccct
    10201 gggcgcggca gttaccgctc cgtatcgcta tggttacccc tactactacg gcgcgtatgg
    10261 ccccgactac tactatgccc cgccgccgac ggtgatctac cagcaggcgc cgcccgccta
    10321 cacggcggcc ccgccgcccg tgcagcgtga ggtcgtgtac gcgcacggca agtacgtgct
    10381 ctacggcgac ggcgtgacgc aggcctggca gtgggtgtgg gtgcccaacg cgccggccag
    10441 cccgcctccg cccccgccgg cgcgctagcc cccgggccgt ccgcctccac tcctcgtccc
    10501 ggccatgcga ctcggcggca ggattgcgct cgtcacgggc gcgcagcagg gtatcggccg
    10561 ggccatcgcc atcgcgctgg gccgcgacgg cgccaacgtc tgcgtcaact tcctggatga
    10621 ccgcggggcc gctgagcgcg tggccgccga gatccggggc cacggcggcc aggcgatgac
    10681 cgtcgcgggc gacgtcgcgc gcagtcagga cgtcgcggcc atcctggcgg cggcgatcgc
    10741 ggagctgggc acgcccgaca tcctcgtcaa caacgcgggc gtcttcccgc gcgcctcgtt
    10801 cctcgatctg gccgagtcgg aatgggacca cgttctcggc gtgaacctca agggcggctt
    10861 cctctgcgcc caggccgcgg cgcgcgccat ggtcgcggcg ggcaagcccg gcgcgatcat
    10921 caacatctcg tcctccgcga tccgcggcga cgcgcgcggc gtgcactact cggccagcaa
    10981 ggcgggcgtg ctcggcatga cccgcgcgat ggccctggcg ctggcgccgc atcgcatccg
    11041 cgtcaacgcc atcgcccccg gcctgaccga cacgcagcag ccccgctacg gcaataccga
    11101 ggagcagatc gcggcgcgcg cgcaggagat cccgctcggc cgcatggccc agcccgagga
    11161 gatcgcgcgg gtggccgtct tcctcgcctc cgaggacgcg gccttcatca cgggcgagct
    11221 catccacgcc aacggcggcg cctatctcgc ctagctcgga ggggggctcc gcccccttcg
    11281 gaacctctcc ccagagacaa gctgcgcggg cgaagcccgc gctcgaagcg ggggccgttg
    11341 gtgtggggat ggagtcctgg cgtagaatcc tggcccatga ccatgacccg gtcgccgcga
    11401 gtggaatcgg ggctaacccg ccgtgttcct ccggcgggga ttcggcccgg tggagacgac
    11461 caatcggctc ggcggcaatt acgtgcgcgt cttccggcag gtggcggcgg cgcggaagga
    11521 ggcggcatga aggacatcac ggtcattcgt ctggaagaca ccgagcgggt gagcttcggc
    11581 ccactgtccc actatcagcc catcgtcggc gacgactcgg gcagcacgcc ggtgcgcacc
    11641 gggatccaga ccgcgcagcc cggctacgcg gccgcggtgc actcgcatcc gtacatcgag
    11701 atcctgcaca tcctggacgg tgtcgcggag gcgtggatcg acggcaagcc cgagtccatg
    11761 gtcgtgctcc ggaagggcga cacgatcgcg gtgccgccgg agacgccgca cgccttccgg
    11821 gtggtgggcg accaggtgct gcgcctgctc ggcactcacg tctccccgaa gcgcatcgtc
    11881 aactacaagg acggagtcac gagcgacgcg cgcggctacc gcagcgaggt gagctaggac
    11941 atgcgactcg gggtcggggt ggccacgatg aacccggacg gctcgccgct caccggggac
    12001 gtgctggtcg gcagcgcgcg gatggccgag cgaatcgggt tcgatagcct ctggttcttc
    12061 gacgcgatga gccggggctt catcctgccc gacccgctga tcggcgcatc ggtggccgcc
    12121 gcggtgacca cccgggccga ggtggggacc tgcgtgctgc aggtgccgct gcgcaacccg
    12181 gtcgagctgg cccatcgcat cctcaccgcg cacctcgtct ccgccggtcg gctcctgctc
    12241 ggcgtgggcg cggggtccac caaggccgac ttcgacgcgc tcgggctcga ctacgccgcg
    12301 cgtctccagg ccttcgagga cgcgctgccg gtgatgcgcc ggctctggcg cggcgagaag
    12361 gtcggcgcgg ccaacctcac cccctggccc gccgcgctgg gcggccccgc ggtgctgatc
    12421 gggagctggg gcggcaagcg ctggatcccg cgggcggccc aggagttcga cgggtggatc
    12481 gcctcggcgg gccggacgaa tctcaacacc ctcgccgagg gcatccagcg ctaccgggcc
    12541 gccgggggca agcgcgcgat cgcgacgaat atctcggtcg atctcacggg gccgaccgac
    12601 ccgtgccccg atgacggccc gttccacctc cggtgcgctc ccgacgtggc cgcgcagcgg
    12661 ctccggcggc tgcacgacct gggcttcgac gacgcggtgc tggtcctcaa gagcgcctcc
    12721 gaggcgaacc tggccgcggc gcgcgcgctc ctgaaatagc cccatcatga ggcagacggc
    12781 cgaggtcgtg gtggtcggcg gcggcgtgaa cggcgcgagc atcgcttacg cgctggccgc
    12841 caagggcgtc aaggtcgtgc tggtggagaa gggcgcgctc gcgggcggcg cctccggccg
    12901 gtcgagcgcg ctggtgcgga tgcactacac gaacgaatgg gacgcgcggc tggcctgggc
    12961 cagctttccg gtgttccgga actgggcgga gctgatgggc gggccaccgg tgttcacccg
    13021 caccggcttc gtcaacgtgg tggcgccccg ctacgcccag caccttcgcc agaacgtcga
    13081 gatgctccgc cgcatcgggg tcgacaccac cgcgatcacg ccgctcgagc tccgggagct
    13141 ccagcccttc gcccacgtgg acgacgtcgg cgcggccgcc tacgagccgg agagcggcta
    13201 cgccaatccg gccgagacgg tggaaggctt ccgccgccga gcggtggagc tgggcgcgcg
    13261 cgtgttcccg tggatcgcgg tgacccggat cgtccggcgc gagagccggg tcaccggcgt
    13321 ggagacggcc gcgggccgga tcgacgcggg ccacgtggtg gtggcggcgg gcgcctggtc
    13381 gcgccggctg tgcgaggaga tcgggctcgc ggtgcccgcg cggcccaagg ccatcgacac
    13441 ggtggcggtg agccgcccgg cggagctgcg cgatccgcac atggtcttca tcgacaacgt
    13501 gcagggcaac tacttccggc cggagagcgg cggtctcacg ctggtcgggg tgccgtgcca
    13561 ggagtgggac atcgacccgg acacgctcgg caccggcctg ccgccggagg cggccggcct
    13621 cggggcgcag ctcctcacgc accgcatccc ggccatggag cgggcgacgc tctcccgcgg
    13681 gtaccgggcc ttcgacggct acagccccga ccgccacgcg atcctgggcg cggtggacgg
    13741 catcgacggg ctgtatctgg ccaccgcctt cagcggctcc gggttcaaga tcgccccggc
    13801 ggtcggtcag tgcatggccg agctcatcac ggatgggcgc gccaagacag tggacatcga
    13861 ggtgttccgc ctcggccgct tcgctcaggg caagacggtg gaggggccgt atccgtacgc
    13921 ggtccggccc gactacgtgg atccggcatc cgcgggaggc gcgcagtgag cgcgggacgg
    13981 ccgccgggcg tcgagttcca caccttcacg gcactcggcc gctgcccgcg caccgggcgc
    14041 ctcggcatcg ccaccgcgac ccgctcgctc gcggtcggcg cgcgcgtccc gcacgtgcgc
    14101 gcggggctcg gcgccatcgc gatcatggcc atcgcggatg cgcggctcgg ctacatggcg
    14161 ctgcggctgc tcgagctcgg gtacaaggcg cccgcggtga tcgaggagct ggtcaagggc
    14221 gatccgtacg cggagtaccg gcagctcggg gtcatcgacg ccgacggctt cgcggccgcc
    14281 cgtaccggca agatgaaccg cgactgggcc ggccatcacg tgcgggacga ccacatcgtg
    14341 ctcggcaacg tgctcaccgg cccacacgtg ctcgacgcga tggagaaggc cttcgcggcc
    14401 gatcccgccg aggatctcga ggagcgcctg ctgcgggcga tcgaggctgg gcgcgacgcg
    14461 ggcgggcagc acggtggcca gcagtccgcc gcgttgctgg tctacgacga ccggcccttc
    14521 gcccacgtgg acctgcgggt ggacgtgcac gtcgagccga tcggcgagct gcggagggtc
    14581 ttcgacgtct accggcccgc gatcccgtac tacaacctgc gtcaggtgga cgcgcgcgtg
    14641 cctccgctcc aggactggct ggccgcccgg ccgcgctgag cgtcccggat cgcgccccag
    14701 acgcgctcgg gcgtgaccgg catctcgagg tgctccacgc ccagttccgc gagggcatcc
    14761 acgatcgcgt tcaccgcggc cccgagcgcc ggggtcgtgc cgccctcgcc gccgccccgc
    14821 aggccgagcg ggttcgcggt cgacggcacc tcgctgatct cggtggcgaa cgagggcagg
    14881 caatcggcgc gcggcatcgc gtactccatg aacgtcgcgg actggagctg gccgctctcc
    14941 gcgtcgtagc gacagtgctc ccagagagcc tggcccaggc cctggacgat cccgccgtgg
    15001 gtctgcccgt cgacgatcag cggattgatg gcgcggccga cgtcgtccac ggaggtgtag
    15061 cggacgacct cgaccacccc ggtctccgga tccacctcca cctcgcacac cgcgcagccg
    15121 tacgggaagg acggcgtgct cacggtctcg tcgccggccc cgtcgagcgg gccgcgcagg
    15181 tcctccggcg cgtcggggcg cccggcggcg gccgcgacct cgaacaggct gatggcgcga
    15241 tcggtgccgc ggacgcggaa gcggccgccc gcgaactcga gatcggccac cgcggactcg
    15301 agccgccacg cggcgagccg cgcgcccttc tccacgatct ggtccgccgc cttcgccatg
    15361 acgacaccgc cgagacgcat tgagcgtccc gagtgtgagc cgccacccac cgcgacgcga
    15421 tcggtgtcgc cgctgatgag gcgcacctgc tcgagtgctc cgcccagcca ctcgacgacg
    15481 agctgggcga agctcgtctc gtggccctgg ccggccgcga gggtgccgat caccacgtcg
    15541 atgcgcccct cgggctgcac cgtgatgtgc gcccgctcgc gcggcgcacc ggtggcgatc
    15601 tcgatgtagt tggcgaggcc gatcccgcgg tagcgcccgc gcgcgcgggc ctcccggcgc
    15661 cgcgcctcga agccgcccca gtcggcgagc gccaccgcgc ggtcctggac cgccacgtag
    15721 tcgccgctgt cgtagacgat cccgaacggg ttggtgtacg gcatcgcggc gctcggcacc
    15781 agattccggc gccgcagcgc gagccggtcg aagccgtggc ggcgcgcggc gaggtcgatg
    15841 agtcgctcca tcacgaacat cacctggggc cggcccgcgc tgcggtaggc ggagagcggt
    15901 gaggtattgc tcagcaccgc gcgcccgcgc accgcggccg cgggcacccg gtagacggtg
    15961 gtggccagct cacgcccctt gttgagcggc acgaaggtca cggtgtgcgc gccgacattg
    16021 ctggtgttca cgccccggag cgcgaggaag cggccgtccg cgtcgagggc cagctccatc
    16081 gaggagacga gatcgcgcgc ctggtagtcg gtcaggaacg cctcgcggcg ctcgcacgtc
    16141 cacttcaccg ggcggccgag gcgccgcgcg gcccacgcga ccagggcgaa ctccggatag
    16201 aagctgttcc gcgtgccgaa gttgccgccc acgtcctccg ccaccacgcg caccgcggcc
    16261 tccggcacgc ccagcacctc cgcgagatcg gccttctgcc gcaccacgcc gcccgagccg
    16321 gcgtagagcg tgtagcgccc ggtcgccgcg tcgtggacgc cgagggccgc gcgcggctcc
    16381 atgggcacgc cggtgatccg cggtacccag gtgtcgagcc ggacggtgtg cgcggccgcc
    16441 gcgaacgcgg cgtcggcggc gcgcgcgtcg cccgcgaccg agtccacaca caggttcgag
    16501 ccgaactcgc tccagaggac gggcgcgccg ggctctcccg cggcgggggt ggcggtgacc
    16561 gacggcagcg gctcgtaggc gacctgcacg cgctcggcgg cgtcggccgc caccgcggcg
    16621 gtctccgcga tcaccatcgc caccgcctcg cccgcgaagc gcgcgacgtc ggcggccagc
    16681 ggcggatgcg gggtgacgag cacgcgccca ccctcgcggc cgccgagcgg gaactcgttc
    16741 gggttggtcg gcaccggttt gtgggggatc gaggcgagcc cgtcggccac cgcgtcgcgt
    16801 ccggtgagga ccgcgagcac ccccggcacg gcgagcgcgg cggccgcctc gatcgagaga
    16861 atgcgcgcgt gggcgtgcgg tgaccggacg agatgcgcgt gggcctggcc cggcaggttg
    16921 aagtcgtcgc tgaagcgacc gcgcccggtc acgaggcgcg cgtcctcctt gcgcggcacc
    16981 ggccggccga agccctgggg tgccgagcgc gtgcgcgcgg tcaggaacga tgcgccggtc
    17041 gtggtcatgg ggccgcgggc gcgtcggccc ggcggccccg caatcttacc tcagcgcggc
    17101 tagcccatca ggccttccga ctcgctgccc aggaagaaca cgccgaggcc gtccgcgtcc
    17161 acgcgccaca ccatcgcggc cacgcgaacc cgtcggccgt tcggcttgac gaactgcagg
    17221 gtcagcgacg tgccgggcag caggtcgccc ttgggccgga gcttcgcgcc ccgcgtgctc
    17281 acgtccacgg tatggaagcg gcaccgctgt ccccggaccc gagcgaagac ggtccacgag
    17341 acactagccc tgcggttgat gcgccgggtg cgttgatgat ggctgctcgc ggttgatgtc
    17401 atggtcctgg cgaatggcgc ggtcatgatc tccatggccc cgggtggagg caaggcccgg
    17461 gccagccgga ggaccgacga gaaatcggag agttagaggc ggatcggccg gacgcgcaat
    17521 cttggatggg aacgcagcta cccagatagg tcgaggcgct ctacgtgatg gacactgcgg
    17581 gaatacgcaa atcgttcccg ccgcctaccc gccggtcgcc ccccgctcgc gcaggaacgc
    17641 gagcccacga cgcgcgtctt ccaccgacag ctcgatcacg ccgggcagcg ccgcgaacgc
    17701 cgggtggctc gccacggata ccacgcccag gccgatggtg cccgcgcccg gaatcgcgtg
    17761 acggtcccgg tgtgcgccgc acgcgagctg ggagtcgttc agatgaaacg cgacgacgcg
    17821 ctcgagcacg ccctccgcgg ccagtgcgtc ggccagtcgc tggcccgaat ccgagcccgc
    17881 gagatcgaag ccggcggcgt ggagatggca ggtgtcgatg agcaggccga gctgctcggg
    17941 cggggcgccg acgtcgcgga gcagccgcgc cagctcggcg atggtctggc ccagctcgct
    18001 gccctcgcag gaattctcga tcaggagccg gggacccgcc ggcatgcgcc cgatcagctc
    18061 gccgagcttt cgggtggcga ggatcagagc ggcgtccgcg ctcggatgcc ctttggcgta
    18121 acccacgtga aagcacaccg cgtcgagccc gacctccgcc gcgaccttga ggtcggcgag
    18181 caggctcgag acggaggagt tgcggatgcg gccttccggg ctcgcgaggt tgacgagcag
    18241 cgagccgtgg gccacgcccc agacgcggcg gccgcggagc ccctcgacca gcgcggcgcg
    18301 gtcggccggc tccggaacgc cgtagttgcc gaagcgcccg ggatcgcgaa ggacggtctg
    18361 gtagacgtcg acgccctcct cgcggagcag cgcgggaatc gcggccagcg gcggatcgac
    18421 ggatagcccg acgcgcaccc cggtactgta gcacccggct cggcgccgtt gacagcctcg
    18481 cgcgatcgcg cgaggctggc acgcatcgac gatgcgcacg gccggtgtgg tggctctgat
    18541 gatgctgacg atggccgggg tcgtcgcttc gtgggccgac cggtggccgg ccccccagac
    18601 gcgcgaggtg tggagcgaat cccgagacta tttcgtccgg gtgattccgg gcacgagcat
    18661 cggagagacg gtgggctttc gcggcgctcc caccgggcgc caggccacgg ccgagctgta
    18721 ccggcgggcg agtgaccgtt cgtatcgcct ggcctggacg gtcagccttc tgaacccggt
    18781 ggccccgatc gatctgttcc tgaccgaccg gggatacctg gtcacgctcg acaactggca
    18841 caacatgggc tatggcaaga tcgtcgcgtt ctacgctccg gacggcaagc tggtccggag
    18901 ccacgccctc gacgatctgt tctcggcggc cgagatcgac aagcttccgg gctcggtgtc
    18961 atcgatctgg tggcgcaagc agatcgcgta cgtccggctt gaccagcaga gcctctatgt
    19021 gtcggtgggc gatggaggcc gcgagttcgt cttcgagacg gagaccggcg cctatcaatt
    19081 ctgcgagacg ggagacggag cctaccgctg ccgcagctcg aatgaccagc gccagtggcg
    19141 agggtacgtc gacccgccgt cccggcgctg agcggcccgt cgacgatcag gcgcgggcga
    19201 ggcgcggcag gaccgccttg ccgaacagct ccaccgtgcg ctccggatcg gccgcaaggc
    19261 tcaccgtgaa catgagctgg gtgacccccg ccgcctcggc ggcgcgcacc tgcgccacgc
    19321 agtcctcggg cgtccctgcg atcgcgaggc gggcgcggag gtagtcgaag aggccgaggc
    19381 gccgggtgag cgccggatcc atgtcccgct gtcgcgtggt gtaggtggcc cgcagctcgt
    19441 gaaccgccgg gacgagatcg ggcgggaccc cgcggccggc cgggtctggc cccaggatgt
    19501 acgccgcgac gaagcccagg atgttcccga gcttctcgag cgcgtcctcg cgccgctcgg
    19561 ccacgtcgag gcatgcgatc gaccagacgt cgatcgcgct cgcgggccgg cgcgtcccgg
    19621 ccgccccctc ggcgatcagc ccccgcaccc ggctcacgtg gtccggcccc agtccatagt
    19681 tgacgaaggc gccgtcggcc gccgcgccgg ccgcgcgcag cgccgccggc cccgaggcgg
    19741 ccgcgtagac gggcaccgcg cgctggatcc aggagaggtg cgtgggggcg ccccgccacg
    19801 acgcgggcgc tccgctcagc gcggtcttca agaacgtgag gccgtcgcgg agatcggagg
    19861 cgcggctggg tgccccgccc acgttggcga cgccgctgtg ccccgcgccg actcccagga
    19921 tggtccggcc gcccgcggcc acgtccaccg aggccgccgc cgacgcggtg atcgagggat
    19981 ggcgcgtgac gagattggtg acgcaggtgg ccagcatcac ccgcgacgtc gcggcggccg
    20041 ccagcgtcat ggccacccac gggtcgagcg cgttgcccgg tgtgtcggcg atgccgacca
    20101 ggtcgaagcc cagctcctcg ccccgccgcg ccatgcgcgt cgtgtactcc ggcgtgtacg
    20161 gccagaagaa gaagccgaag cgcatggagc ccgcgctcag ggcttggcgg ggggataatc
    20221 gcgcgtgtag ggcgggctct tgaggaattc ctccggcttg taggtccaga actgcgacac
    20281 cgccgggtag gtgtggatca cggtggtggc gagcctggcg cccaccttgt cgacgcgcag
    20341 gatgtagacg ttctcggtgg gattgcccca gtcgtcgagc ttgatgggcc cgcgcggatc
    20401 cggcgtgctc tcgatggacc ggcggatggc ggcgacgagc ttctcgcggt cctcgacctg
    20461 gccgccgagc gcgttcgcag cctcggtgat ccagcgcccc gagctgtaca tgatggcgtg
    20521 gaagtaggcg ggggtccgcc cgagcttggc ctcggccatc ttcatgaagg tctggttcgc
    20581 cgggttctgc agcgtgggac tccagagcag ggaccccacg acgccgacgg cttcctcgcc
    20641 catgccccgc agcgcgctct cgtcgatcat gacgccggtg ccgatgagcg gggtcttgcc
    20701 cttgagtgcg gactcgccgt actgtttcac gaagcgcacc gcctggccgg ccacgaagac
    20761 cgcgcagacc gcgtccacgt ccttctggac ctgcgcgaga tagggcgcga agtcgagcgc
    20821 gttgagcggc acccagatct tctggaccac cttgccgccc gcgtcctcga acacgcgctg
    20881 gaagccgccc gcctcctcgt ggccgaagcc gttgtccatg gcgatggtgg ccacgcggcg
    20941 gtacttgagc gtccgggccg cgtagtcgcc cagtgggtgc atgatctggc tggccgagaa
    21001 gttggagcgg aggatccact tggcgacctt gcgcttggtg aggtcgtcgg gcgtggtcag
    21061 gaacagggtg gggaggcggt cgcgctcgat cagcggcacc aggctgttgc ccacgttgac
    21121 gagcaggatg ccggtcatga cgtggatctt gtcgtggtcc accagcttgc gg
//
LOCUS       DASUFA010000024         9071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1147919, whole genome shotgun sequence.
ACCESSION   DASUFA010000024 DASUFA010000000
VERSION     DASUFA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147919"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFX87_00885"
     CDS             complement(<1..609)
                     /locus_tag="VFX87_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX4993484.1"
                     /translation="MAPDLRRSTIPQGIPTPVAVPRLGLRAVRLSEAWFARLLVAPAV
                     LAIVAVVAVPLAYSLWLSFTDVNLLRGGGAAIELFGMRLPLYRWVGLKNYLQVFSDPL
                     YWDSLWRTLYFVGAFVIEATLVGLGVALVLNERFPGRPLMRSLLLVPWSLSRIVVGLL
                     WIGILDFEFGAFNGYMQRLGLMTGSIAFFKDGFSALNVLVSVY"
     gene            complement(610..1908)
                     /locus_tag="VFX87_00890"
     CDS             complement(610..1908)
                     /locus_tag="VFX87_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4993485.1"
                     /translation="MSTISRRRFLGNAGLLGAGAVLADGGLRGARADKPLNFSGWVFK
                     PDTVKDYVDFYNKKHGGQVKYEAIPWTAYHPTMETRAFAGEIVDVMYCNHNNRERWYE
                     NGLIRPVDDLPGTDELKKKMTPANLDSLKSKDGSKLLGLPYFTSLFVLIYNEPMLQQA
                     GIKAPAKSWDELVEHCTKLKRDKVSDTPFLPNWNNSPSGTMPQFMTDCFSEGASVFDA
                     KNRVIVDQEPGAARAMERWQKVYKAELVNPEVLTKTSSTDTHRIFWTGRYAYHTNHSY
                     YLKTIAGEPENSKLAPKKAKMAMYPGTGQTYMWTDSYVVNAKSKALEDAWKLTRFLGG
                     NLNGDWYVQRQWCLISGLDNPYPEMYDHPEIISSYDRWIDLKLLREQYKKGKVIAAYK
                     EPWYGEYDTKAVATVHNIIRGTTTVPKGLKDLVALQKSLA"
     gene            complement(2027..3250)
                     /locus_tag="VFX87_00895"
     CDS             complement(2027..3250)
                     /locus_tag="VFX87_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX4993486.1"
                     /translation="MRLHPLPVLRPPGALASRVASPPYDVVSRSEAAALARDNPLSFL
                     HVGRAEIDLPETVDAHDPRVYREARRALDRLVAEGALRRAAPALYLYREVMAGRAQIG
                     VVGGVHVDDYAADVIRKHETTRPDKEDDRTRHLLALAAHAEPVLLLYRGRPEIDRLVV
                     AATAAPPLYDFATADGVAHTVWRVDDPAPYERAFERVPQAYVADGHHRSASAWRAARE
                     LRARHTGPAHEEEYDWFPAALVPADQLRILAYNRVVTDLAGRAPAEVLAQLGRVGRLA
                     VTDDPVPPRPATFCVYLGGRWHRLELAEDSIDRSDPIRALDVSLLQERVLGPVLGIAD
                     QRTDRRIDFVGGIRGARALAARVDSGEAALGISLHPTTVEQLMAVSDAGHIMPPKSTW
                     FEPKLLSGLFVHPFA"
     gene            3368..3619
                     /locus_tag="VFX87_00900"
     CDS             3368..3619
                     /locus_tag="VFX87_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX4993487.1"
                     /translation="MPLYLDVHHKIDGLSADSIAHAHRQDLEVQKKYGVRYLRYWYDE
                     GTGKVFCLVEAPSREAAAAVHREAHGSVADEIIEVKEGS"
     gene            3634..6654
                     /locus_tag="VFX87_00905"
     CDS             3634..6654
                     /locus_tag="VFX87_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4993488.1"
                     /translation="MRCGECGHENSADARFCNGCGAPLRAVCASCAAPNPPGSRYCRE
                     CGSALGAGPAGARFVSPQSYTPPHLAEKILTSRAAVEGERKQVTVLFADLKSSMELLS
                     DRDPEEARKLLDPVLERLMGAVHRFEGTVNQVMGDGIMALFGAPLAHEDHAVRACYAA
                     VAMQEEIRRYTEELRRAQGIEVQIRVGLHSGEVVVRAIGNDLRMDYSAVGQTTHLAAR
                     MEQLAAPGSTRLTADTVRLAEGFVHVTALGPVPVKGVAEPVEVFELLGAAGRRTRLEA
                     AADRGLTRFVGRRAELDQLRESFETARGGRGQVVAVVGEPGVGKSRLFWEFVHSHRLG
                     GALVIPTTAVSYGKGTPYSSVIDLARAYFGIESRDDTRQVREKVTGRLLALDEALRPV
                     LPALLALLDVTTGDAAWDRLAQSQRRAATADAIKAVLLRESQTRPVVITFEDLHWADA
                     ESQAVIDGLIAGLPTAAIFVLLNYRPEYQHPWGNKTYYAQLRLEPLRPESADEMLRAM
                     LGERPDLGQVKRLLIERTDGNPFFLEESVRTLVETGLLAGERGAYRPARPVDSIQIPT
                     TVQALVAARIDRLPAEDKQVLQTAAVIGKDVLLSVLRDISDLSEDALHAVLRRLQGAG
                     FVSELALFPEAEYTFRHPLTHEVAYAGLLHERRRGLHARVVDAFERLHPDRRAERLHW
                     LAHHAFRGEVWGKALLYLRHAISDTPEAMDAMEVILAPGGPGHLWWTGDHARARETSL
                     RDLAVSRSFRVFQQQTAALLRLGQIHHSLGDFPRAEEFLRQNVESLEGELRLDRLGLP
                     GLPAVSSRTWLAWCLAERGELAQAAEHAETGLRIAEEARHARSLIVATMGLGVVQMAA
                     GRLEAAVSTLERGLVLDQVEQIATLFPFVAAPLGCAYARIGRTAEGVTMLERALAQAE
                     TLKLAANQSRALVWLGQAHLLAGRADRARELAERALAHARARSERGHVAWALHLRATA
                     TPSSAADSYREALALASELGMAPLCAACRVALAG"
     gene            complement(6643..7413)
                     /locus_tag="VFX87_00910"
     CDS             complement(6643..7413)
                     /locus_tag="VFX87_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4993489.1"
                     /translation="MDGVTMHEEVSAAIAAAKAAGEVAMRYYRSGFEVTIKPDQTPVT
                     QADREAEQAIKRVLGRAFPNDGFLGEELGEEGPRDRRWIIDPIDGTKNFIRRIPIWAV
                     LIGLEEAGEVTVGVVFNPASDELLWARKGDGAWSGGARLRVSGCRAMKDAFLLHSDLH
                     LLREAGYWERFVRMLDASGRTRGFGDYYGYGLVASGKAEIYLETDLKPWDAAPVKILV
                     EEAGGRLTDFAGRPTIYGGSVLATNGHLHDLALRALAG"
     gene            complement(7401..7787)
                     /locus_tag="VFX87_00915"
     CDS             complement(7401..7787)
                     /locus_tag="VFX87_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4993490.1"
                     /translation="MDDALKEKTRKTAHMLFHSIKSGVGFDTWKKFDKELARELSMFF
                     TGKLYSREVISQKQRELCAVASLTVLNRRNEVRAHIHAALNVGATRQEVAEVIFQQVT
                     YGGMPVVVEALEVFKEVLQERGEWTE"
     gene            7896..8291
                     /locus_tag="VFX87_00920"
     CDS             7896..8291
                     /locus_tag="VFX87_00920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4993491.1"
                     /translation="MAASIQRYAAKGIWDPPGYSQAVKVTGAQTILYIAGQVAYDDKG
                     GPAHRGDFKAQARATFQALKAQVEAGGGTMDSIVKINTYLTDIRHRADLAAVREEFLG
                     KKSPASTLVAVAALAMPEWMIEIEAVAVI"
     gene            8299..>9071
                     /locus_tag="VFX87_00925"
     CDS             8299..>9071
                     /locus_tag="VFX87_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX4993492.1"
                     /translation="MAELNPYIFRAYDVRGKVGIDITPPVFELVGRAYGTLVTRRGGQ
                     AVALGMDNRTSSRALKEAFATGVLSTGLDVVDIGVNHTPLLYFATAHWKLDGGATITG
                     SHNPVSDNGVKMVHAGAAPLTESEIQGLLATIQSGDFERGVGTRATRDPRDEYWDAIT
                     ARVTVRRRLKVVVDAGNGIAGVFAPELLRRLGCEVVELYCESDGTFPNHLPDPEMEEN
                     MRDLVARTLEVGADVGVAYDGDADRVGIVDEKGRRHEADL"
BASE COUNT         1325 a         3207 c         3214 g         1325 t
ORIGIN      
        1 gtacaccgac accagcacgt tcagcgcgct gaagccgtcc ttgaagaagg cgatcgagcc
       61 ggtcatcagg ccgagccgct gcatgtagcc gttgaaggcg ccgaactcga agtcgaggat
      121 gccaatccag agcagcccca cgacgatgcg ggacagcgac cacggaacga ggaggaggct
      181 ccgcatcagg ggccggcccg ggaaccgctc gttcaggacc agcgccaccc cgaggccgac
      241 cagggtcgcc tcgatcacga aggcgcccac gaagtagagc gtgcgccaga gggaatccca
      301 gtagaggggg tcggagaaga cctggaggta gttcttcagc cccacccagc ggtagagggg
      361 caggcgcatc ccgaacagct cgatggccgc gccgccgccg cggagcaggt tgacgtcggt
      421 gaacgagagc cagagcgagt acgcgagcgg gaccgcaacc accgccacga tggcgaggac
      481 ggcgggcgcg accaggagcc gggcgaacca ggcctcggac agacgcaccg cccgcagccc
      541 gaggcggggc accgccaccg gagtcgggat cccctggggg atcgtcgagc ggcggagatc
      601 gggggccaac taggccagcg acttctggag cgcgaccagg tccttcaggc ccttgggcac
      661 cgtcgtggtg ccgcggatga tgttgtgcac ggtggcgacg gccttcgtgt cgtactcgcc
      721 gtaccagggc tccttgtagg ccgcgatgac cttgcccttc ttgtactgct cgcgcaggag
      781 cttgagatcg atccaccgat cgtaggacga gatgatctcg ggatggtcgt acatctcggg
      841 gtacgggttg tcgaggcccg agatcaggca ccactgccgc tggacgtacc agtcaccgtt
      901 caggttcccg cccaggaacc tcgtgagctt ccacgcgtcc tcgagggcct tgctcttggc
      961 gttcaccacg taggaatcgg tccacatgta ggtctggccg gtccccgggt acatcgccat
     1021 cttggccttc ttgggggcga gcttcgagtt ctcgggctcc cccgcgatgg tcttgagata
     1081 gtacgagtgg ttggtgtggt aggcgtagcg cccggtccag aagatgcggt gggtgtcggt
     1141 ggacgaggtc ttggtgagca cctcggggtt caccagctcc gccttgtaga ccttctgcca
     1201 gcgctccatc gcccgcgcgg ccccgggctc ctggtccacg atcacccggt tcttggcgtc
     1261 gaagacgctg gcgccctccg agaagcagtc ggtcatgaac tggggcatgg ttcccgacgg
     1321 gctgttgttc cagttgggca ggaacggggt gtcgctgacc ttgtcccgct tgagcttggt
     1381 gcagtgctcg accagctcgt cccagctctt ggccggggcc ttgatgccgg cctgctgcag
     1441 catcggctcg ttgtagatga ggacgaagag gctggtgaag tagggcaggc cgaggagctt
     1501 gctgccgtcc ttgctcttca ggctgtccag gttcgccggc gtcatcttct tcttgagctc
     1561 gtcggtgccg ggcaggtcgt cgaccggccg gatcagcccg ttctcgtacc agcgctcgcg
     1621 gttgttgtga ttgcagtaca tcacgtccac gatctcgccc gcgaaggcgc gcgtctccat
     1681 ggtggggtgg taggccgtcc acgggatcgc ctcgtacttc acctgcccgc cgtgcttctt
     1741 gttgtagaaa tcgacgtagt ccttcacggt gtcgggcttg aagacccacc ccgagaagtt
     1801 cagcggcttg tccgcccggg cgccccggag gccgccgtcc gccagcaccg cccccgcccc
     1861 gagcaatccc gcgttcccga gaaagcgccg cctgctgatc gtgctcatga atacgtcctc
     1921 ctttgaaggt gactgcctcg gcgatgacga gcgatcgatc cggtgaacgc tggcgggatt
     1981 ctgggacagg ggcccgcgct tgtcaagacg agccgtggcg aacggatcac gcgaacgggt
     2041 ggacgaagag accgctcagg agcttgggct cgaaccacgt gctcttgggc ggcatgatgt
     2101 ggcccgcgtc ggagacggcc atgagctgct ccaccgtggt gggatggagc gagatcccca
     2161 gcgccgcctc gcccgagtcc acgcgcgcgg ccagcgcgcg cgcgccccgg atgccgccca
     2221 cgaagtcgat gcgccggtcg gtgcgctggt cggcgatgcc cagcaccggg cccagcacgc
     2281 gctcctgcag cagcgagacg tcgagggcgc ggatcggatc ggaccggtcg atcgagtcct
     2341 ccgccagctc gagccggtgc cagcgcccgc cgaggtagac gcagaaggtg gccgggcgcg
     2401 gcggcaccgg atcgtcggtg acggccagcc ggcccacgcg gcccagctgc gcgaggacct
     2461 ccgccggcgc gcgcccggcc aggtccgtca ccacgcggtt gtaggcgagg atgcggagct
     2521 ggtcggcggg cacgagcgcc gcggggaacc agtcgtactc ctcctcgtgc gcaggccccg
     2581 tgtgccgcgc ccgcagctcg cgcgcggccc gccacgcgct ggccgaccga tgatggccgt
     2641 cggccacgta cgcctgcggc acccgctcga aggcccgctc gtatggggcc ggatcgtcca
     2701 cgcgccacac ggtgtgtgcg actccgtccg cggtggcgaa gtcgtagagc ggcggcgcgg
     2761 cggtcgccgc caccaccaga cgatcgatct ccggacggcc ccggtagagc agcaggaccg
     2821 gctcggcgtg ggccgcgagc gcgagcaggt gccgggttcg atcgtcctcc ttgtcgggtc
     2881 gggtcgtctc gtgcttgcgg atgacgtcgg ccgcgtagtc gtccacgtgc acgccgccga
     2941 ccacgccgat ctgcgcgcgg cccgccatga cctcgcggta gaggtagagc gccggcgcgg
     3001 cgcggcgcag cgcgccctcg gccaccagcc ggtccagggc ccgccgcgcc tcccggtaga
     3061 cgcgcgggtc gtgggcgtcc accgtctcgg gcaggtcgat ctcggcgcgg ccgacgtgga
     3121 ggaacgagag cgggttgtcc cgggccaggg ccgccgcctc gctccggctg acgacgtcat
     3181 agggcggcga cgcgacgcgc gaagccaggg cgccgggagg ccgcaggacc gggagtgggt
     3241 ggagccgcac gcgcagcaac ctaccgttcg cgcccacccg ggtcaagatc ggcccgacgc
     3301 cttgacaccc ttcggccccc catgctgcaa tgacgcacat tcatctcgac ccctcggagg
     3361 gcccacgatg ccgctgtacc tcgacgtgca tcacaagatc gacggcctca gcgccgattc
     3421 gatcgcacac gcgcaccggc aggacctcga ggtgcagaag aagtacggcg tcaggtacct
     3481 gcgctactgg tacgacgagg gcaccggcaa ggtcttctgc ctggtcgagg cgcccagccg
     3541 cgaggcggcc gccgccgtcc atcgcgaggc gcacggcagc gtggccgacg agatcatcga
     3601 ggtcaaggaa ggctcgtaga gccccccggt ccggtgcgct gcggcgagtg cgggcacgag
     3661 aactccgcgg acgcccggtt ctgcaacggc tgcggcgccc cgctccgcgc ggtgtgcgcg
     3721 tcgtgcgccg cccccaatcc ccccgggagc cgctactgcc gcgagtgcgg cagcgcgctc
     3781 ggcgccggcc cggcgggcgc gcggttcgtc tcgccccaga gctacacgcc gccccatctc
     3841 gccgagaaga tcctgacctc gcgcgccgcg gtcgaaggcg agcgcaagca ggtcaccgtg
     3901 ctcttcgccg acctgaagag ctcgatggag ctgctctcgg accgggatcc cgaggaggcc
     3961 cgcaagctcc tcgacccggt cctcgagcgt ctgatgggcg cggtgcatcg cttcgaaggc
     4021 accgtcaacc aggtcatggg tgacgggatc atggccctgt tcggggcgcc cctcgcccac
     4081 gaggaccacg cggtgcgcgc ctgctacgcc gcggtggcca tgcaggagga gatccggcgc
     4141 tacaccgagg agctgcggcg cgcccagggg atcgaggtgc agatccgggt cggcctgcac
     4201 tcgggcgagg tcgtggtgcg cgcgatcggc aacgacctgc gcatggacta ctccgcggtc
     4261 ggccagacca cgcacctcgc cgcgcgcatg gagcagctcg cggcacccgg cagcacgcgg
     4321 ctgaccgcgg acaccgtgcg cctggccgag ggcttcgtcc acgtgaccgc cctggggccc
     4381 gtgccggtga agggcgtggc cgagcccgtg gaggtcttcg agctgctcgg cgcggcgggg
     4441 cggcgcaccc gcctggaggc ggccgccgac cgcggcctca cgcgcttcgt cggacgccgg
     4501 gccgagctcg accagctccg cgagagcttc gagaccgcgc ggggcggccg cgggcaggtg
     4561 gtggcggtgg tcggcgagcc cggggtcggc aagtcccgcc tgttctggga gttcgtccat
     4621 tcccaccgcc tcgggggcgc cctcgtcatc ccgaccaccg cggtctctta cggcaagggg
     4681 acgccctact cgtcggtgat cgatctcgcg cgggcctact tcggcatcga gtcgcgcgac
     4741 gacacccgcc aggtgcgcga gaaggtaacg ggccggctgc tcgcgctgga cgaggccctg
     4801 cggccggtcc tccccgcgct gctggccctg ctcgacgtga ccaccggcga cgcggcctgg
     4861 gaccggctcg cgcagtccca gcgccgggcc gcgacggcgg acgcgatcaa ggcggtcctc
     4921 ctgcgcgaga gccagacgcg gccggtcgtc atcaccttcg aggatctcca ctgggcggac
     4981 gcggagagcc aggccgtcat cgacggcctc atcgcgggcc tcccgaccgc ggcgatcttc
     5041 gtgctcctca actaccgccc cgagtaccag cacccctggg gcaacaagac ctactacgcc
     5101 cagctccgcc tcgagccgct gcggccggag agcgcggacg agatgctgcg cgcgatgctc
     5161 ggggagcgcc cggacctggg ccaggtgaag cgccttctca tcgagcggac cgacggcaac
     5221 ccgttcttcc tcgaggagag cgtgcggacc ctggtggaga cgggcctcct cgccggagag
     5281 cgcggcgcct accgtccggc gcgacccgtg gacagcatcc agatccccac gacggtgcag
     5341 gcgctggtcg cggcgcgcat cgatcggctg cccgcggagg acaagcaggt gctgcagacc
     5401 gcagcggtca tcggcaagga cgtgctcctg tccgtgctgc gagacatcag cgatctctcc
     5461 gaggacgcgc tccacgcggt gctgcgccgg ctccagggcg cgggcttcgt ctccgagctg
     5521 gcgctcttcc ccgaggccga gtacacgttc cggcacccgc tcacccacga ggtggcctac
     5581 gccgggctgc tgcacgagcg gcgccgcggc cttcacgccc gcgtggtgga cgccttcgag
     5641 cgcctgcacc cggaccggcg cgcggagcgc ctgcactggc tcgcccacca cgccttccgg
     5701 ggcgaggtgt ggggcaaggc cctcctctac ctccgccacg cgatcagcga cacccccgag
     5761 gcgatggacg ccatggaggt catcctggcc cccggcggcc cgggacacct ctggtggacc
     5821 ggcgatcacg cgcgggcacg ggagacctcg ttgcgcgatc tggcggtcag ccggagcttc
     5881 cgggtcttcc agcagcagac ggccgccctg ctgaggctgg ggcagatcca tcactccctg
     5941 ggcgacttcc cgcgcgcgga ggagttcctc cgccagaacg tggagagcct cgagggcgag
     6001 ctgcgcctcg accggctcgg cctgcccggc ctgccggcgg tcagctcgcg gacgtggctg
     6061 gcctggtgcc tcgccgagcg cggggagctc gcgcaggcgg ccgagcacgc ggagaccggc
     6121 ctccgcatcg cggaggaggc gcgccacgcg cgcagcctga tcgtggccac catgggcctg
     6181 ggcgtggtgc agatggcggc ggggcgcctc gaggcggcgg tgtccacgct cgagcgcggc
     6241 ctcgtgctcg accaggtcga gcagatcgcg accctgtttc cgttcgtggc cgcgccgctg
     6301 ggctgcgcgt acgcccgcat cgggcggacc gcggagggcg tgacgatgct cgagcgggcc
     6361 ctcgcgcagg cggagaccct gaagctcgcg gccaaccagt cgcgggcgct ggtctggctc
     6421 ggccaggcgc atctgctcgc cgggcgcgcc gaccgcgcgc gcgagctggc cgagcgcgcc
     6481 ctggcccacg cacgcgcgcg cagcgagcgt ggccacgtgg cgtgggcgct gcacctgcgg
     6541 gccaccgcca cgccctcctc cgccgcggac tcctaccgcg aagcgctcgc gctcgcctcc
     6601 gagctcggca tggcgccgct ctgcgccgcc tgccgggtgg ccctagccgg ctagcgcccg
     6661 cagcgccagg tcgtgcaggt ggccgttggt ggccagcacc gagccgccgt agatcgtggg
     6721 cctccccgcg aagtcggtga ggcggccgcc cgcttcctcc accaggatct tcaccggggc
     6781 cgcgtcccag ggcttgagat ccgtctcgag gtagatctcg gccttgccgg aggccaccag
     6841 gccgtagccg tagtagtcgc cgaagccgcg cgtgcgaccg gaggcgtcga gcatgcgcac
     6901 gaagcgctcc cagtagccgg cctcgcggag gagatggagg tcggagtgga gcaggaaggc
     6961 gtccttcatc gcgcggcagc ccgacacgcg gaggcgggcg ccgccggacc aggcgccgtc
     7021 gcccttgcgc gcccagagga gctcgtcgct ggccggattg aagacgacgc ccacggtcac
     7081 ctcacccgcc tcctcgaggc cgatcagcac cgcccagatc gggatgcgcc ggatgaagtt
     7141 cttggtgccg tcgatgggat cgatgatcca tcgccggtct cggggcccct cctcgccgag
     7201 ctcctcgccc aggaatccgt cgttgggaaa ggcccgcccc agcacccgct tgatggcctg
     7261 ctccgcctcg cgatcggcct gggtgaccgg cgtctggtcg ggcttgatgg tgacctcgaa
     7321 gccgctgcgg tagtaccgca tcgcgacctc gcccgccgcc ttggcggccg cgatcgccgc
     7381 gctcacctcc tcgtgcatcg tcactccgtc cactcgcccc gctcctgcag gacctccttg
     7441 aagacctcga gggcctccac cacgaccggc atgccgccgt aggtgacctg ctggaagatc
     7501 acctcggcca cctcctggcg ggtcgccccc acgttgagcg cggcgtgaat gtgggcgcgc
     7561 acctcgttgc gacggttgag cacggtgagc gatgccaccg cgcacagctc gcgctgcttc
     7621 tgggagatga cctcgcggct gtagagcttg ccggtgaaga acatgctgag ctcgcgcgcg
     7681 agctccttgt cgaacttctt ccaggtatcg aagcccaccc cgctcttgat ggagtggaac
     7741 agcatgtggg cggtcttgcg tgtcttctcc ttgagggcgt cgtccatggg ggtctcctga
     7801 aactccgtac gtctcgttct tgatcgttcg cgctatatta gcctaagcac cgagactcca
     7861 tcacccgtca ctcatcatcc atcaggggag atcgtatggc cgcctccatc cagcgctacg
     7921 ccgccaaggg catctgggat ccgcccggct attcgcaggc cgtgaaggtc accggcgccc
     7981 agaccattct ctacatcgcg ggccaggtcg cctacgacga caagggcggg ccggcccacc
     8041 gcggggactt caaggcgcag gcccgcgcca ccttccaggc gctcaaggcg caggtcgagg
     8101 cgggcggcgg caccatggac agcatcgtca agatcaacac ctatctgacc gacatccggc
     8161 atcgcgccga cctggccgcc gtccgcgagg agttcctcgg caagaagtca cccgcgtcca
     8221 cgctggtggc ggtggccgcc ctcgccatgc cggagtggat gatcgagatc gaagcggtcg
     8281 cggtcatata gccgcgtgat ggccgagctg aacccgtaca tcttccgcgc gtacgacgtg
     8341 cgcggcaagg tgggcatcga catcaccccg ccggtgttcg agctggtggg gcgggcctac
     8401 ggcacgctgg tgacgcgccg gggcgggcag gcggtcgcgc tcgggatgga caaccggacg
     8461 tcgtcgcggg cgctcaagga ggccttcgcg accggggtgc tctccaccgg gctcgacgtg
     8521 gtggacatcg gcgtcaatca cacgcccctg ctctacttcg ccaccgcgca ctggaagctc
     8581 gacggcggcg ccaccatcac cggcagccac aatccggtct cggacaacgg ggtcaagatg
     8641 gtgcacgcgg gcgcggcgcc gctgaccgag agcgagatcc aggggctgct cgccaccatc
     8701 cagtcggggg atttcgagcg gggcgtgggc acgcgcgcca cccgcgatcc gcgcgacgag
     8761 tactgggacg cgatcaccgc ccgggtgacc gtgcggcgaa ggctgaaggt cgtggtggac
     8821 gcgggcaacg ggatcgcggg cgtcttcgcg cccgagctgc tgcgccgcct gggctgcgag
     8881 gtggtggagc tctactgcga atccgacggc accttcccca accatctccc cgatccggag
     8941 atggaggaga acatgcggga cctggtggcc cggaccctcg aggtgggcgc cgacgtgggc
     9001 gtggcctacg acggcgacgc cgaccgggtc ggcatcgtgg acgagaaggg ccggcgccac
     9061 gaggcggacc t
//
LOCUS       DASUFA010000025         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_577838, whole genome shotgun sequence.
ACCESSION   DASUFA010000025 DASUFA010000000
VERSION     DASUFA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577838"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFX87_00930"
     CDS             complement(<1..715)
                     /locus_tag="VFX87_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4993493.1"
                     /translation="MRAVAIQAHGGLDQVKLRTDWPEPKAGPGQAVVEVKACGLNYLD
                     IFVLQGMPGLPVTMPRIPGGDIAGLVHSVGAGVSREWVGRRVLIDPHIKPGGALGEHA
                     NGGLCERIAIEAENLIHMPDAVTFEQAAALPIAYGTAYRMMITRGHVQAGELVLILGA
                     SGGVGTGCVQIAKNLKARVIACASSANKLRRLKELGADHVIDYTKEDFSQKAWELSGK
                     RGVDVLVNYTGGDTWVPSLR"
     gene            complement(836..>2105)
                     /locus_tag="VFX87_00935"
     CDS             complement(836..>2105)
                     /locus_tag="VFX87_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993494.1"
                     /translation="GRWTVVGVSTTVNALAWGARSTFALFYVAMLEEFAWGRGPTALG
                     YSLSWLGFVVFAPVAGWLSDRWGTRTVVTGGGVILGAALALTGQVTSLAQYYLCFGLL
                     GGAGIAGMLIPSTTIVTRWFVRARGTAMGVLSTGPGSAVVFYPLAAWLIAILGWRMAL
                     VSFGCIVAAATVLLALLYREPAAAVDRSAGGADTKSSAGPRALGEAWTLRRALRSVRL
                     WAAFTMTALGVIGFQIMATHQVAHAADHGVPRDTVVWLFALAAGCMMAGNVLGGWLSD
                     RVGRGWVFVLGTVVAILGILSFALLRGPEDRVLLLLYAVSGFGFGMRIAQLSTIPADV
                     FAGPHLGAILGVVQAGGGLGGAIGPYLAGWLFDATNSYQLAFLAACVAVAGSAVAAWF
                     AARPMPGAPAPGRREPHSTREQRSGGPRVD"
BASE COUNT          357 a          774 c          718 g          256 t
ORIGIN      
        1 cgcggaggct cggcacccag gtgtcgccgc ccgtgtagtt cacgagcacg tccacgcccc
       61 tcttgcccga aagctcccac gccttctggg agaagtcttc ctttgtatag tcgatgacgt
      121 ggtccgcgcc cagctccttg aggcgccgga gcttgttcgc ggacgacgcg caggcgatca
      181 cccgggcctt gagattcttg gcgatctgca cgcagccggt gcccacgccg ccgctggcgc
      241 ccaggatcag taccagctca cccgcctgca cgtgcccacg cgtgatcatc atccgataag
      301 ccgtgccgta ggcgatgggc agcgcggcgg cctgctcgaa ggtgaccgcg tcgggcatgt
      361 ggatcagatt ctccgcctcg atcgcgatgc gctcacagag cccgccgttg gcgtgctcgc
      421 ccagcgcccc gcccggcttg atatgcgggt cgatcagcac gcgccggccg acccattcgc
      481 gcgagacacc ggcgcccacc gagtggacga gaccggcgat gtcgccgccg gggatgcgcg
      541 gcatcgtgac cggcaggccc ggcatgccct ggagcacgaa gatgtcgagg tagttgaggc
      601 cgcaggcttt gacctccacc accgcctggc cggggccggc cttgggctcg ggccagtccg
      661 tgcggagctt gacctggtcg aggccgccgt gcgcctggat cgcgaccgcg cgcataccta
      721 ggcgtctccc cgagtcatgg ccccttgatg taacactgtc ggccctgatg tggcaaccaa
      781 ggtgggaatg gcaggtacgg agggtcccgg gccgcgtcgc cggccctcat ccgcctcagt
      841 cgacgcgcgg accgcccgac cgctgctccc gcgtggagtg cggctcgcga cggccgggtg
      901 ccggcgcgcc gggcatgggc cgcgccgcga accacgccgc gacggccgag cccgcgaccg
      961 cgacacaggc ggcgaggaac gctagctgat aactgttggt ggcgtcgaag agccatccgg
     1021 cgaggtaggg gccgatggca ccgcccaggc cgccccccgc ctggaccacc ccgaggatgg
     1081 cgcccaggtg cggtccggcg aacacgtcgg cgggaatcgt ggagagctgg gcgatccgca
     1141 taccgaagcc gaatccggac accgcataga gaaggaggag aacgcgatcc tcggggcccc
     1201 gcaacagggc gaaactcaga atcccgagaa tcgcgacgac cgtgccgagc acgaagaccc
     1261 acccgcgccc gacccgatcc gagagccagc cgccgagaac gttgcccgcc atcatgcacc
     1321 ccgcggcgag ggcgaagagc cacaccaccg tgtctcgcgg aacgccgtgg tccgccgcgt
     1381 gagcgacctg atgggtggcc atgatctgga agccgatgac tccgagcgcg gtcatggtga
     1441 aggccgccca gagccggacg ctccgcagcg cgcgtcgaag cgtccacgcc tcaccgaggg
     1501 cccgcgggcc cgccgaggac ttcgtgtcgg caccgccggc cgagcgatcc acggcggccg
     1561 ccggctcgcg gtagaggagc gcgagcaaca cagtcgcggc cgcgacgatg cacccgaaac
     1621 tgaccagcgc catgcgccag cccaggatcg cgatgagcca tgcggccagc gggtagaaga
     1681 ccaccgcgct gcccggtccg gtgctcagca cgcccatcgc cgtaccgcgg gcccgcacga
     1741 accaccgcgt cacgatcgtc gtcgatggga tgagcatgcc ggcaatgccc gcgccgccga
     1801 gcaggccgaa gcacaggtag tactgcgcga gagacgtcac ctggcccgtg agcgccaggg
     1861 ccgccccgag gatcacgccg ccgcccgtca cgaccgtccg cgttccccag cggtcggaga
     1921 gccagccggc caccggcgcg aagacgacga aacccagcca gctcagcgag tacccgagcg
     1981 cggtcgggcc gcgaccccac gcgaactcct cgagcatcgc gacgtagaag agcgcgaacg
     2041 tggatcgcgc cccccacgcc agggcgttga cggtggtcga cacgccgacc accgtccatc
     2101 ggccg
//
LOCUS       DASUFA010000026         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1035999, whole genome shotgun sequence.
ACCESSION   DASUFA010000026 DASUFA010000000
VERSION     DASUFA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035999"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFX87_00940"
     CDS             complement(<1..362)
                     /locus_tag="VFX87_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX4993495.1"
                     /translation="MAATWTWNTALTGVVPPLISPLGDGGAADAGAMAALVEHVVAGG
                     CTGLFVLGGCGEGAWLTTAQRDAVVRAAVKAAAGRVPVLAAVMLPASGPATEAARRAR
                     GEGADAVVVGSPYYFAVDG"
     gene            405..2078
                     /gene="ggt"
                     /locus_tag="VFX87_00945"
     CDS             405..2078
                     /gene="ggt"
                     /locus_tag="VFX87_00945"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4993496.1"
                     /translation="MTEKRGAIASRGMVVTNHPLGSAAGAEMLAAGGNAVDAAIAALF
                     ALTVVEPMMVGIFGAGMTHLRLGDGRHLVIDNYTTAPATSRPDMYRPVSDSWPDYLRV
                     EGDLNLVGLLSVGVPGTLKAWSEAMARFGRLDLETVMQPAIRHAERGFRATPYLVEAI
                     TETAADLARFPETARTFLPGGSPLKPGDLVVMPDYAATLRHLAAKGPGALYGGDLGAR
                     VADYMRRAGGIITLDDLARYETIERRPIVGRYRGFEIAGAPPPTAGGLHLVEMLNILE
                     GFDLRGLGFGTADYFHLIAEVLKIGFADRNACTGDPAFVDIPMERLLSKEHAAARRAE
                     IRPDRASDFSVTGAADARAASTSAHTTHVTTADADGNVVAMTQTINNLFGSKATVPET
                     GILLNNTMALFDPHPGTPASIAPGKRVTSSMAPTMVFRDGQPAWALGLPGGVRIFTSV
                     LQAVVNLIDHGMTLQQAVEAPRIWSQGQELEMERDIPAVIRDEVAARGHQVVAVPAVA
                     GGMNAVAFTPDGLLEGAACWRADGTPIGVSGGLARPGIRFRPDANRRPG"
     gene            complement(2075..2551)
                     /locus_tag="VFX87_00950"
     CDS             complement(2075..2551)
                     /locus_tag="VFX87_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993497.1"
                     /translation="MTRRRLSSALTFFWKVGVPLVWIPGIAACAVSAFFVTPGKPPDP
                     ELESLQLSFLGVSLVGAPIFLWFAAGLKRVRREGTDLLVSNYWRELRIPVTEIKQVYQ
                     SWAVMPWRVVIEMRAPTELGETFIFVPRFRDGIGHAALGGRHPVVEEIRVMCDAAR"
     gene            complement(2587..2955)
                     /locus_tag="VFX87_00955"
     CDS             complement(2587..2955)
                     /locus_tag="VFX87_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4993498.1"
                     /translation="MQITGASPQLRTTDLAASIRFYTTKLGLTLEFQYEDFYAGIRAG
                     NQVFHLKLSGEKDPSIAFVDRGEHFHLYFETDDAAAVAAGLKKNGVSLLKELHETPWE
                     TRELAVKDDQGHTLYFGERR"
BASE COUNT          461 a         1112 c         1023 g          413 t
ORIGIN      
        1 ccgtccacgg cgaagtagta gggcgagccc accaccaccg cgtcggcgcc ctcgccgcgc
       61 gcgcgccgcg ccgcttccgt cgccggcccg ctggcgggca gcatcaccgc cgcgagcacg
      121 ggcacgcggc ccgccgccgc cttcaccgcc gcccgcacca ccgcatcgcg ctgggcggtg
      181 gtgagccagg ccccttcgcc gcaaccgccg agcacgaaga ggccggtgca gccgcccgcc
      241 accacgtgct ccaccagcgc ggccatggcc ccggcatcgg cagcgccgcc gtcgccgagc
      301 ggggagatga gcggcgggac gacgccggtg agcgcggtat tccaggtcca ggtggccgcc
      361 atgggggcga gcgtagcacg gcgcgatccg ctatgatccg ccgcatgacc gaaaaacgag
      421 gggccatcgc gtcgcgcggc atggtggtga ccaatcaccc gctgggctcc gcggcgggag
      481 cggagatgct cgccgcggga ggcaacgcgg tggacgcggc catcgccgcg ctcttcgcgc
      541 tcaccgtggt ggagccgatg atggtgggca tcttcggcgc cggcatgacg catctgcgcc
      601 tcggcgacgg ccgccatctc gtcatcgaca actacacgac ggcgcccgcc acctcgcggc
      661 ccgacatgta ccggcccgtt tccgactcgt ggccggacta tctgcgggtg gagggcgacc
      721 tgaatctggt cggccttctc tcggtgggcg tgcccggcac gctcaaggcc tggtcggagg
      781 cgatggcgcg cttcggccgc ctggacctcg agacggtgat gcagcccgcc atccgccacg
      841 cggagcgcgg cttccgcgcc acgccctatc tggtggaagc gatcacggaa accgccgcgg
      901 acctcgcgcg ctttccggaa accgcgcgca ccttcctgcc cggcgggtcg cccctcaagc
      961 cgggcgatct cgtcgtcatg ccggactacg cggccacgct gcgccacctc gcggccaagg
     1021 gccccggcgc gctctacggc ggcgacctcg gcgcgagggt ggcggactac atgcgccgcg
     1081 ccggcggcat catcaccctg gacgatctcg cgcgctacga gaccatcgag cggcggccca
     1141 tcgtcggccg ctaccgcggc ttcgagatcg cgggcgcgcc gccgcccacc gcgggcgggc
     1201 tgcatctggt cgagatgctg aacatcctcg agggcttcga cctgcgcggc ctgggcttcg
     1261 gcaccgcgga ctacttccac ctcatcgcgg aagtgctcaa gatcggcttc gcggaccgca
     1321 atgcctgcac cggcgatccc gccttcgtgg acatcccgat ggagcggctc ctctccaagg
     1381 agcacgcggc ggcccggcgc gcggagatcc gcccggaccg cgcgagcgat ttttcggtga
     1441 ccggggcggc cgacgcgcgg gccgcttcca cgtccgccca caccacccat gtgacgacgg
     1501 cggacgccga cggcaacgtg gtggcgatga cccagaccat caacaatctc ttcggttcca
     1561 aggccacggt gccggaaacc ggcatcctgc tcaacaacac catggcgctg ttcgacccgc
     1621 atcccggcac gccggcctcc atcgcccccg gcaagcgcgt gaccagcagc atggcgccca
     1681 ccatggtatt ccgcgacggc cagcccgcct gggcgctggg gctgccgggc ggggtgcgca
     1741 tcttcacttc ggtgctccag gcggtggtga acctcatcga ccacggcatg acgctccagc
     1801 aggcggtgga agcgccgcgc atctggagcc agggccaaga gctcgagatg gagcgggata
     1861 tccccgccgt catccgcgac gaagtcgccg cgcgagggca tcaggtcgtc gcggtgcccg
     1921 cggtggcggg cgggatgaac gcggtggcct tcaccccgga cggcctgctg gagggcgcgg
     1981 cctgctggcg cgccgacggc acgcccatcg gcgtgagcgg cgggctggcg cggccgggga
     2041 ttcgcttccg gccggacgcg aaccgccggc ccggctagcg cgccgcatca cacatcaccc
     2101 ggatctcctc gaccacggga tgccggccgc cgagcgcggc gtggccgatc ccgtcgcgaa
     2161 atcgggggac gaagatgaag gtctccccga gctcggtcgg cgcgcgcatc tcgatcacca
     2221 cgcgccaggg catgaccgcc cagctctggt acacctgctt gatctccgtg acgggaatcc
     2281 gcagctcccg ccagtagttc gagacgagca gatcggtgcc ctctcgcctc actcgcttca
     2341 gccccgcggc gaaccagaga aagatcggcg cccccacgag ggacacgccc aggaaggaaa
     2401 gctgcagcga ctcgagctcg gggtccgggg gcttccccgg cgtgacgaag aacgcggaca
     2461 ccgcacaggc cgcgatgccg gggatccaga ccagggggac cccgaccttc cagaagaagg
     2521 tcagcgccga ggacagccgc cgtctcgtca tcgccgtcgg ctccgcgacc ccgcgcgcgc
     2581 cgaaatctag cgccgctccc cgaagtagag cgtgtgtccc tgatcgtcct tgaccgcgag
     2641 ctcccgtgtt tcccagggcg tttcgtgcag ctccttcagc agcgacaccc cgttcttctt
     2701 caagccggcc gccaccgcgg cggcatcgtc ggtctcgaag tagaggtgga agtgctcccc
     2761 ccggtccacg aacgcgatcg acgggtcctt ctcgcccgag agcttgaggt gaaagacctg
     2821 attccccgcc cggatgcccg cgtagaagtc ctcgtactgg aactcgagcg tcaagccgag
     2881 cttggtggtg tagaagcgga tcgaggccgc cagatcggtg gtccgaagct ggggactggc
     2941 gcccgtgatc tgcatcagtc ctccacgtgg atcaaggtag ctccatgata tgcgtgcagt
     3001 gggcgaccc
//
LOCUS       DASUFA010000027         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1036458, whole genome shotgun sequence.
ACCESSION   DASUFA010000027 DASUFA010000000
VERSION     DASUFA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036458"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFX87_00960"
     CDS             <1..516
                     /locus_tag="VFX87_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993499.1"
                     /translation="GLLLSISSAAEAVEHQGRALVRLAAGHGGRSDEVPDAAWAGLGG
                     VVDGPLLLRVIGEPRRLLDWVGEAHVAAARAGVQAMVLAQPGHGVVQLSIRAGFDAGR
                     VVGELVLPLRRALEAEGGSLIVERSPIEIKTECDVWGDIQLEILEIMTRIKAEFDPAG
                     VLNPGRFVGGL"
     gene            576..1796
                     /locus_tag="VFX87_00965"
     CDS             576..1796
                     /locus_tag="VFX87_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX4993500.1"
                     /translation="MDGLRACVHCGICLPACPTYRVLGEEMDSPRGRVYLMRAAAEDR
                     IGLTPIMARHLDLCLGCRACETACPSGVPFGQLLEATRGQFERRGVTAPTRDGATLRF
                     ILETFPHPGRLGPMLHALRMYQWTGVQAVVRGLRLLAPFKKLQAMEALLPRVPRPGAP
                     LPELIPARGPRRGRAGLLLGCAQRFLYPDVNADTVRLLAAAGYDVVVPPAQGCCGALH
                     LHAGRLDEFRAMARRLMPAFADVDVVVVNAAGCGSALKEYGHWLPDDAARLFAERVRD
                     VSEVLAGCDLPLRALPQTVTYHDACHLVHGQKVRAQPRELLRRIPGLTLVDLTDSELC
                     CGSAGVYNLLEPGMAGELGGRKAARIRETGVRVVAAGNPGCLMQIHQHCRQAGLDVEV
                     VHPVTLLARALEER"
     gene            1793..2125
                     /locus_tag="VFX87_00970"
     CDS             1793..2125
                     /locus_tag="VFX87_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993501.1"
                     /translation="MKIRVRDHVKVAADRMAKIGLSTTARTQLDLYCVAPGQSQAPHR
                     HDGEDKIYYVLEGSGRFSVGGAEERLVAGEAAVAAAGVEHGLVNDSAELLLVLVLVAP
                     PPPHMKRS"
     gene            2131..2871
                     /locus_tag="VFX87_00975"
     CDS             2131..2871
                     /locus_tag="VFX87_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX4993502.1"
                     /translation="MAAPVRAALMITCLGDMLFPEVGVAMVRLLRRLGVQVEFPPGQT
                     CCGMPLFNSGYHQDAALVAARTVKLFEGAQHVVVPSGSCAWMVRTEYPGLLKHDPALR
                     AAADSLARRTRELSQFIVEVLGVTTIESSFRGKVTYHDSCHLLRGLGESASPRTLLRG
                     VRGVELVELPGADECCGFGGSFSVRLPEVSSSILDKKLANVESTGARCLVACDAGCLM
                     QMGGGLSRRGSAVKAVHLAQVLAGEVEG"
     gene            2868..>3224
                     /locus_tag="VFX87_00980"
     CDS             2868..>3224
                     /locus_tag="VFX87_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX4993503.1"
                     /translation="MSDALLRTPFPERAGAALKDTFLQEALKIATTKFIDLRKESFAG
                     FPQGEALRDRARAIKEATLQHLDVHLERLADNVERLGGHVHWATTAEEAREMVLRLCR
                     DRGVRMAVKSKSMATEE"
BASE COUNT          396 a         1119 c         1225 g          484 t
ORIGIN      
        1 gggctgctcc tctccatcag cagcgcggcc gaggccgtgg agcaccaggg ccgggccctg
       61 gtgaggctcg cggccggaca cgggggtcgg agcgacgagg tgcccgacgc ggcgtgggcc
      121 ggccttggtg gcgtggtgga cggacctctg ctgctgcgcg tcatcggcga gccccggcgc
      181 ctgctggact gggtggggga ggctcacgtg gcggcggctc gggccggcgt ccaggcgatg
      241 gtcctcgcgc agccggggca cggcgtggtg cagctctcca tccgggccgg cttcgacgcg
      301 gggcgcgtcg tgggcgagct ggtgctgccg ctcaggcgcg cgctcgaggc ggaaggcggt
      361 agcctgatcg tcgagcgcag cccgatcgag attaagactg agtgtgatgt ctggggtgac
      421 atacagcttg aaatactgga aataatgacc cgcatcaagg ccgagttcga cccggcgggc
      481 gtgctcaatc ccggccgctt cgtggggggc ctgtgagcca ggcgcagccg gtcggtgtcg
      541 ggctcggggc cttcgacgct caggatccgc cgtccatgga cggcctccgc gcctgcgtgc
      601 actgcgggat ctgcctgccg gcgtgcccga cctaccgggt cctcggcgag gagatggact
      661 cgccgcgggg ccgggtctat ctgatgcgcg cggccgcgga ggaccggatc gggctcacgc
      721 ccatcatggc ccggcacctc gatctctgcc tcggctgtcg ggcctgcgag accgcgtgcc
      781 cctccggggt gccgttcggc cagctcctcg aggcgacgcg gggccagttc gagcgccgcg
      841 gcgtcacggc ccccacgcgc gacggcgcca cgctacgatt catcctggag accttcccgc
      901 atcccgggcg gctcggcccc atgctccacg cgctccgcat gtaccagtgg actggcgtgc
      961 aggcggtggt gcgcgggctc cgcttgctgg cccccttcaa gaagctgcag gcgatggagg
     1021 cgctgcttcc gcgggtgccg cggcccggcg cgccgctgcc ggagctgatc ccggcgcgcg
     1081 gcccccgccg cggtcgcgcc ggcctgctgc tcggctgcgc gcagcgcttt ctgtatcccg
     1141 atgtcaacgc cgacaccgtg cggctcctcg cggcggccgg ctacgacgtg gtggtgccgc
     1201 cggcccaggg gtgctgcggg gcgctccacc tgcacgccgg ccgcctcgac gagttccgcg
     1261 cgatggcccg ccggctgatg ccggccttcg ccgacgtgga cgtcgtggtg gtgaacgcgg
     1321 cgggctgcgg ctcggcgctc aaggaatacg gtcactggct gccggacgac gcggcccggc
     1381 tcttcgccga gcgggtgcgg gacgtctccg aggtgctggc gggctgcgat ctgccgctcc
     1441 gcgcgctgcc gcaaaccgtg acgtatcatg acgcctgcca cctggtacac ggccagaagg
     1501 tccgcgcgca gccgcgcgag ctgctgcgcc gcattcccgg cctcaccctg gtggacctga
     1561 cggactccga gctctgctgc gggagcgcgg gggtctacaa tctgctcgag cccgggatgg
     1621 ccggggagct gggcgggcgc aaggcggccc gcatccgcga gaccggcgtc cgggtggtcg
     1681 ccgcgggcaa tcccggctgc ctgatgcaga tccaccagca ctgtcgacag gcggggctgg
     1741 acgtcgaggt cgtgcacccg gtgacgctcc tggcccgggc gctggaggag cgatgaagat
     1801 tcgcgtgcgg gatcacgtga aggtggcggc ggaccggatg gccaagatcg gcctctccac
     1861 caccgcgcgc acccagctcg atctctactg cgtcgcgccg ggtcagtcgc aggcacccca
     1921 ccggcacgac ggcgaggaca agatctacta cgtgctcgag ggatccgggc gattctcggt
     1981 gggcggcgcc gaggagcggc tggtggccgg cgaggcggcg gtggcggccg cgggcgtcga
     2041 gcacggcctc gtcaacgact cggccgagct gctcttggtg ctggtgctgg tcgcgccgcc
     2101 tccgccgcac atgaagcggt cctgaggccc atggccgccc cggttcgcgc cgcgctgatg
     2161 atcacctgcc tgggggacat gctcttcccc gaggtggggg tcgcgatggt gcggctcctc
     2221 cggcggctcg gcgtgcaggt ggagtttccc cccggccaga cctgctgcgg gatgccgctc
     2281 ttcaactcgg gctaccacca ggacgcggcc ctggtggcgg cgcgcaccgt gaagctcttc
     2341 gagggagcgc agcacgtggt ggtgccgtcc ggctcctgcg cctggatggt gcggaccgag
     2401 tatccaggcc tgctcaagca cgatccggcg ctgcgcgcgg ccgcggactc gctggcccgg
     2461 cgcacgcggg agctctcgca gttcatcgtg gaggtcctcg gcgtcaccac gatcgagtcg
     2521 tcgttcaggg ggaaggtcac ctaccacgac tcctgccacc tgctccgcgg gctcggcgag
     2581 tcggcgtcgc cccgcaccct gctgcgcgga gtgcgcgggg tggagctggt cgagctgccg
     2641 ggagcggacg agtgctgcgg cttcggcggc tccttctcgg tgcgtctgcc cgaggtgtcc
     2701 tcgtccatcc tcgacaagaa gctggccaac gtggagtcca ccggcgcgcg ctgcctggtg
     2761 gcgtgcgacg cgggctgcct catgcagatg ggcggcgggc tgtcccggcg cggctccgcg
     2821 gtgaaggcgg tgcacctggc ccaggtgctc gccggggagg tcgaggggtg agcgacgcgc
     2881 tgctgcggac gccctttccc gagcgggccg gcgcggccct caaggacacg ttcctgcagg
     2941 aagcgctcaa gatcgccacc accaagttca tcgatctccg caaggagtcg ttcgcgggct
     3001 tcccccaggg cgaggcgctg cgggaccgcg cgcgcgcgat caaggaggcc acgctccagc
     3061 atctcgacgt ccacctggaa cggctcgccg acaacgtcga gcggctgggc gggcacgtgc
     3121 actgggccac caccgccgag gaggcccgcg agatggtcct gcgcctctgc cgggatcggg
     3181 gcgtgcgcat ggcggtgaag tcgaagtcca tggccaccga ggag
//
LOCUS       DASUFA010000028         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_922434, whole genome shotgun sequence.
ACCESSION   DASUFA010000028 DASUFA010000000
VERSION     DASUFA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922434"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..649
                     /gene="folK"
                     /locus_tag="VFX87_00985"
     CDS             110..649
                     /gene="folK"
                     /locus_tag="VFX87_00985"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX4993504.1"
                     /translation="MFLSVGSNLGDRLDCLRRAVEQLRAMQDLRFLDASPLYRTEPWE
                     RPPGRPDEDAGWFFNCAVVIETELPPATLLARLQEVEAALGRERRGGTPEAQRYEPRP
                     LDIDILLYDDRVISGPEHLHIPHLLMHERRFVLRPLADLAPDLEHPVLYQTIQELLDL
                     LEDEHEVRESDLPRRWFDD"
     gene            682..1203
                     /locus_tag="VFX87_00990"
     CDS             682..1203
                     /locus_tag="VFX87_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4993505.1"
                     /translation="MADDEFRQQALQHLDALHNLAVYLTRNGSEAEDLVQETYVRAMR
                     FSHRFQPGTHLRAWLFQILRNTFLTFYRLRERESALSEDGVPEGEAPMFHDAPGRDSE
                     SAGAHVDLDRALAKLPEEFKTPLLLAEVEGLALEDVARIMDCPVGTVKSRIFRAKERL
                     RGYLKDYQQQDKQ"
     gene            1301..2020
                     /locus_tag="VFX87_00995"
     CDS             1301..2020
                     /locus_tag="VFX87_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993506.1"
                     /translation="MTRENMEPHEDDGGIGRLLRERLPRHPAPPVLRAAVVEALMPGR
                     PRQPWWVLWLPPAFSAVATALIMLLYLAPSLPSAPGADPMQFMTRAVLAEHARNTFWG
                     ETRSDVVPVALPRAMEESGIALSWVFTGDEDIQLINAKPTYLEGRRGIELAYQDAAGH
                     TVTYVIVPGGSVTLPERGREQIDRWRPVVKRENGFSLILWRQQGMLCVLASDLVSDDD
                     LARFKEYFVKVRSSTEPYAVY"
     gene            complement(2017..3219)
                     /locus_tag="VFX87_01000"
     CDS             complement(2017..3219)
                     /locus_tag="VFX87_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4993507.1"
                     /translation="MSVRGVLLGAFVAGLGVSISLAETALALLTLHLLWRLRDPAERA
                     AARWPLWPPVLAFSAVTILSALLSRHPVASLESAKGLLLVAALYVTADAVRDPVTAGR
                     FLFGVTLAATVAAVVGLLQVGLCPGLNAPAAGPRWLYHRCDRAHGFFSIYMTLAGVLS
                     LVLLATLPRLLPGIGGLRRFGAPWLVMLGGLVATYTRGAWIGFAAGVLTVAASMRRGR
                     WLLLAGLVLLAAGALMAPYELRHRFLSMADPEEAGVRERVYMWRSGLAMWRERPVLGV
                     GPGGVKREYPPYALPEAVKKRTGHVHSTPLQILVERGVLGLAAWLAIWAAFFTRCVGL
                     LRRLPAEATAERVLVVGSLAAIVGFLMGGLSEYNFGDSEVVMVAWALMALPWATQQAR
                     EPRASRAG"
     gene            complement(3216..>3332)
                     /locus_tag="VFX87_01005"
     CDS             complement(3216..>3332)
                     /locus_tag="VFX87_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX4993508.1"
                     /translation="LEKRIQYDLEYIERWSLWLDVKIIAMTVVRVLVDRNAY"
BASE COUNT          546 a         1180 c         1175 g          431 t
ORIGIN      
        1 gacgaagtcc gggtgcgggt gcgcaagctc acgcccccga tggagggcct ccagggcatc
       61 cccggggtcg agctgactcg acggagacgg tagcgagcac atggcgcgag tgtttctctc
      121 ggtggggtcg aacctgggcg accggctgga ttgcctgcgc cgcgccgtgg agcagctccg
      181 cgccatgcaa gatctgcgat tcctggatgc ctcgccgctc taccggaccg agccctggga
      241 gcggccgccc ggccggcccg acgaggacgc cggctggttc ttcaactgcg cggtcgtgat
      301 cgagaccgag ctgccgcccg ccacgctgct cgcgcggctg caggaggtcg aggcggccct
      361 gggccgcgag cggcgtgggg ggacgccgga ggcccagcgc tacgagcccc gcccgctcga
      421 tatcgacatc ctgctctacg acgatcgcgt gatcagcggc cccgagcacc tgcacatccc
      481 gcatctcctc atgcacgagc gccggttcgt gctgcgccct ctggccgacc tcgccccgga
      541 cctggagcac ccggtcctct accagaccat ccaggagctg ctggacctgc tggaggacga
      601 gcacgaggtg cgggagtcgg acctgccgcg ccggtggttc gacgactagc gtgcccccgt
      661 accctgtggc gctgatgtca cgtggctgac gacgagttcc ggcagcaggc cctccagcac
      721 ctggacgccc tccacaacct ggccgtctac ctgacccgga acggctccga ggcggaggac
      781 ctggtccagg agacctacgt gcgggccatg cgattttccc accggttcca gccgggaacc
      841 catctgcggg cctggttgtt ccaaatattg agaaacacgt tcctgacgtt ctaccggctc
      901 cgggagcggg agtctgctct ctcggaggac ggggtcccgg aaggcgaggc gccaatgttc
      961 cacgacgccc ccggccggga tagtgagagc gccggggccc acgtggatct ggaccgggct
     1021 ctggcgaagc tgcctgagga gttcaagacg ccgctcctgc tggccgaggt cgaggggctg
     1081 gcgctggagg acgtggcgcg catcatggac tgccccgtgg gcaccgtgaa gtcccggatc
     1141 ttccgcgcca aggagcggct gcgcgggtac ttgaaagact accaacaaca ggacaagcag
     1201 tagcagggcg aggcgcagcc gaaggcgagc cggcagtggc agggcgaggc gcagccgaag
     1261 gcgagccgag cgaataagag aaagggaaac caacggagtc atgacgcgcg agaacatgga
     1321 gcctcacgag gacgacggcg ggatcggccg gttgctgcgc gagcggctgc cacgtcatcc
     1381 cgcgccgccc gtcctgcgtg ccgcggtggt cgaggcgctg atgcccggcc gaccgcggca
     1441 gccctggtgg gtgctctggc tgccgcccgc gttctcggcg gtggctaccg cgctgatcat
     1501 gctgctctac ctcgcgccct cgctgccctc ggcgcccggc gccgacccga tgcagttcat
     1561 gacccgcgcc gtcctcgccg agcacgcccg caacaccttc tggggcgaga cgcgctccga
     1621 cgtggtcccc gtcgcgctgc cccgggcgat ggaggagagc ggcatcgcgc tgagctgggt
     1681 cttcacgggc gacgaggaca tccagctcat caacgccaag cccacatacc tcgaaggccg
     1741 gcgcggcatc gagctggcct accaggacgc ggccggtcac accgtgacct acgtcatcgt
     1801 cccgggcggc agcgtgaccc tgcccgagcg cgggcgcgaa cagatcgacc ggtggcggcc
     1861 ggtggtgaag cgtgagaacg gcttcagcct gatcctgtgg cgccagcagg gcatgctctg
     1921 cgtgctggcc tccgatctcg tctccgacga cgacctcgcg cgcttcaagg agtacttcgt
     1981 gaaggtgcgg tcgtcgaccg agccctacgc ggtctactag cccgcgcgcg acgcgcgggg
     2041 ttcccgggcc tgctgcgtcg cccatggcaa cgccatcagg gcccaggcga ccatgacgac
     2101 ctcggagtcg ccgaagttgt attcggagag gccgcccatc aggaagccca cgatcgcggc
     2161 caggctgccc accacgagca cgcgctcggc ggtggcttcg gccggtagcc gtcgaagcag
     2221 gccaacgcat cgggtgaaga aggcggccca gatcgcgagc cacgcggcca ggcccagcac
     2281 tccgcgctcg accaggatct gcagcggcgt gctgtgcacg tggccggttc gcttcttgac
     2341 cgcttccggc agcgcgtagg gcgggtactc ccgcttgacg ccgccggggc cgaccccgag
     2401 caccggccgc tcccgccaca tcgcgagccc gctgcgccac atgtatacgc gctcccgcac
     2461 ccccgcctcc tcgggatcgg ccatgctgag gaagcggtgg cgcagctcgt agggtgccat
     2521 gagcgcgccc gccgccagca ggaccaggcc cgccagcagg agccaccgcc cgcgccgcat
     2581 gctcgcggcc accgtcagca cgccggccgc gaacccgatc caggcgcccc gggtataggt
     2641 ggcgacgagc ccccccagca tcacgagcca gggcgccccg aaccgacgca gcccgccgat
     2701 gccgggcagg agacgtggca gggtcgcgag caggacgagc gagaggacac cggccagcgt
     2761 catgtagatg ctgaagaagc cgtgcgcgcg gtcacagcgg tgatagagcc agcgcggtcc
     2821 cgccgccggc gcgttcaggc cgggacagag gcccacctgg agcaggccga ccaccgcggc
     2881 cacggtggcg gcgagcgtca cgccgaagag aaagcgcccc gccgtcaccg gatcgcgcac
     2941 cgcgtcggcc gtgacgtaca gggccgcgac gagcaagagc cccttggccg actcgaggct
     3001 cgccaccgga tggcgcgaga gcagcgcgga caggatggtc accgcgctga aggcgagcac
     3061 gggcggccag agcggccagc gggcggccgc gcgctcggcc ggatcgcgca gacgccagag
     3121 caggtgcagc gtgaggaggg ccagcgcggt ctcggcgagg ctgatggaca ccccgaggcc
     3181 ggcgacgaag gcgccgagca gaacgccccg gacgctcaat aggcgttccg gtccaccagc
     3241 acccgcacca ccgtcatcgc gatgatcttg acgtcgagcc agagggacca gcgctcgatg
     3301 tactcgaggt cgtactgaat gcgcttctcg ag
//
LOCUS       DASUFA010000029         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_808451, whole genome shotgun sequence.
ACCESSION   DASUFA010000029 DASUFA010000000
VERSION     DASUFA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808451"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFX87_01010"
     CDS             <1..414
                     /locus_tag="VFX87_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4993509.1"
                     /translation="LIVPEAAMIFAFGVFLLRQFFVTMPRELEDAALIDGAGPWQVLS
                     RIFIPLSQPVLAALAIFAFRSAWNDFLWPLIAVNKPEMFPLPVALALLRSAYSSESYG
                     PIMAGAALSALPLLVVFIVANRRIVEGVRLSGLKG"
     gene            complement(429..1361)
                     /locus_tag="VFX87_01015"
     CDS             complement(429..1361)
                     /locus_tag="VFX87_01015"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993510.1"
                     /translation="MNVGFIGLGLMGGSMASNLQAAGHALFVYDVRKAAAAPHLAAGA
                     VWQDTPRAVAEAAEVVFTSLPGPPEVEQVAVGADGLLHGVRAGSAVFDLSTNAPALVR
                     RLDGLFAARGAHVLDAPVSGGPVGAKTRRLAIWVGGERALYDRFRPLLEAMGDQPYYV
                     GPVGAGAVAKLVHNCASFTIRAVLAEVFSMGVKAGVAPDLLWQAVRQGNSGRRRTFDV
                     LAEKFLPGEFEPPSFALKLGHKDVSLATALGREHGVPMRLANLALEEITEALGRGWGD
                     RDSSSFMLLEEERAGVEIKVPRERLRQILEQDGR"
     gene            1490..2377
                     /locus_tag="VFX87_01020"
     CDS             1490..2377
                     /locus_tag="VFX87_01020"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4993511.1"
                     /translation="MALTFRKLHPHFVAEVSPVDLRRVHDPETLAAIRAGVDEYAVLV
                     FRDQPFTDAEQLDFAQRLDGMLHTKLGISALQKNRFGNEALGDISNLDENGEIMKSDN
                     RRRMYGLGNRLWHTDASFQDPAGRYSMLSAKVVPPVDADTEYADMRAAYDALPEGEKA
                     RLEGLRVHHSIAYSRQTLGFEFSESEQDALKGAIHPLVRTIPGSKRRSLYVASHASRI
                     IDWPVPEGRLLLRELIEHATRPEFVYRHQWRVGDLVIWDNRATMHRARPFDDAKYRRE
                     LRRVTTLDVEQPAGVPSGA"
     gene            2427..4058
                     /locus_tag="VFX87_01025"
     CDS             2427..4058
                     /locus_tag="VFX87_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEX4993512.1"
                     /translation="MRTRYAPRNRRRILCVSPRYAPSFGTFHYSYPLFGGRIRAFMPP
                     QGLLVIAAYLPESWEVRFVDENSREATAEELAWADAIFLSGMHVQRPFIEEVIARAHR
                     LGKPVVLGGPSVSGCPEHYPGADLIQVGEMGDSTDAIIAWLDGHTGRPREQLRFDTGS
                     RLPLTDFPLPAYHLIALRQYFISSIQFSSGCPYTCEFCDIPALYGRNPRLKTAAQVLA
                     ELDALVAGGAVAIYFVDDNFIANQKAALELLPHLVEWQRRNRFPVRFACEATLNIAKN
                     ERVLALMRDAGFNVVFCGIETPEPEALRSMSKDQNLRMPILEAVRKLNDYGLEVVSGI
                     ILGLDTDTEATADHLIEFIEASEIPMLTINILYALPKTPLWDRLAAEGRILPDGSRES
                     NVIFRLPEETVLAMWRRCIAAAYAPEAIYRRFAHNLAHTFTKRAAYPRSSHRASWRNV
                     VSGLALLGRIVWRIGVRGDYRRTFWRVAGPALRAGQVEEMIQAAVVSHHLIEFTRQCL
                     RGAQEASFYAPSLPRPPAEPPTLHPIRLRREEVSS"
     gene            4082..>4505
                     /locus_tag="VFX87_01030"
     CDS             4082..>4505
                     /locus_tag="VFX87_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4993513.1"
                     /translation="MPTPDQRASVVRKDLDNVIHPIVPHKQLEQQQLVIVSAKDSTVV
                     DADGREYLDGMAGLWCVNIGYGRTELADVAAEQMRQLSYYPHTAMNLPAAALAEKVNG
                     LLGGDNHVYFVSSGSEANEAAFKFARQYARHEYPQQNRY"
BASE COUNT          654 a         1718 c         1471 g          662 t
ORIGIN      
        1 ctcatcgtgc cggaggcggc catgatcttc gccttcgggg tcttcctgct gcgccagttc
       61 ttcgtgacga tgccgcgaga gctcgaggac gccgccctca tcgacggcgc gggcccctgg
      121 caagtcctct ctcggatatt catcccgctg tcgcagccgg tgctcgccgc gctggccatc
      181 ttcgccttcc gctcggcgtg gaacgacttc ctgtggccgc tcatcgccgt gaacaagccg
      241 gagatgttcc cgctgccggt cgcgctcgcc ctgctccgta gcgcctacag ctcggagtcc
      301 tacgggccca tcatggcggg ggcggcgctc agcgcgctgc cgctgctggt ggtcttcatc
      361 gtcgccaacc gccgcatcgt cgagggcgtg cgcctgagcg gcctgaaggg ctagcgatca
      421 ggggcgcttc accgcccgtc ctgctcgagg atctgccgga ggcgctcccg gggcaccttg
      481 atctcgacgc ccgcgcgctc ctcctccagc agcatgaagc tgctcgagtc ccggtcgccc
      541 cagccgcggc cgagggcttc ggtgatctcc tcgagcgcga gattggccag ccgcatcggc
      601 acgccgtgct cgcggccgag cgcggtggcc agcgagacat ccttgtgccc gagcttcagc
      661 gcgaaagacg gcggctcgaa ctccccgggc aggaacttct cggccagcac gtcgaaggtg
      721 cggcggcggc cgctattgcc ctggcgcacc gcctgccaga gtagatccgg ggccacgccc
      781 gccttcacgc ccatcgagaa cacctcggct agcaccgcgc ggatcgtgaa gctggcgcag
      841 ttgtgcacga gcttcgccac cgcgcccgcg cccaccgggc ccacgtagta gggctggtcg
      901 cccatcgcct cgagcagcgg ccggaaccgg tcgtacaggg cgcgctcgcc gcccacccag
      961 atggcgagcc gccgcgtctt cgcgcccacc gggccgccgc tcaccggcgc gtcgagcacg
     1021 tgggccccgc gcgccgcgaa gaggccgtcg agccgccgga ccagcgcggg cgcattggtg
     1081 gagagatcga agaccgccga gcccgcgcgg accccgtgga gcaggccgtc cgctcccacg
     1141 gccacctgct cgacctcggg cgggcccggc agcgaggtga acaccacctc cgcagcttcc
     1201 gccaccgcgc gcggggtgtc ctgccagacc gcccccgcgg ccaggtgcgg ggcggcggcc
     1261 gccttccgca cgtcgtacac gaagagcgca tggccggcgg cctgcaggtt ggacgccatg
     1321 ctgccgccca tgagacccag tccgatgaat ccgacgttca tgtgggatag cctcccgccg
     1381 cgcatcttgg catgccccgg cgcggcttgt cgacggggcc ggcccgcgcg tggaccgttt
     1441 gcttgactcc ccggcccggt cggcctagat tcttggcggg aggcgcgtca tggccctgac
     1501 attccgcaag cttcacccgc acttcgtcgc cgaggtcagc ccggtggatc tccgtcgcgt
     1561 ccacgacccg gagaccctcg ccgcgatccg cgccggcgtg gacgagtacg ccgtcctcgt
     1621 cttccgcgac cagcccttca ccgacgccga gcagctcgac ttcgcccagc ggctggacgg
     1681 gatgctccac accaagctcg gcatcagcgc gctgcagaag aaccggttcg gcaacgaggc
     1741 gctcggcgac atctcgaatc tcgatgagaa cggcgagatc atgaagtcgg acaaccggcg
     1801 ccgcatgtac ggcctcggca accggctctg gcacaccgac gcctccttcc aggacccggc
     1861 cggccgctac tcgatgctct cggccaaggt cgtcccgccg gtggacgcgg acaccgagta
     1921 cgcggacatg cgcgcggcct acgacgccct ccccgaaggg gagaaggcgc gcctcgaagg
     1981 cctgcgcgtg caccactcga tcgcctactc ccgccagacc ctgggcttcg agttctcgga
     2041 gagcgagcag gacgccctca agggcgcgat ccacccgctc gtccgcacga tcccgggctc
     2101 caagcgccgc tcgctctacg tggcctccca cgcgtcgcgg atcatcgact ggccggtgcc
     2161 cgagggccgc ctgctgctgc gcgagctcat cgagcacgcg acccggcccg agttcgtcta
     2221 ccgccaccag tggcgcgtgg gcgatctcgt gatctgggat aaccgcgcca ccatgcaccg
     2281 ggcgcgcccg ttcgacgacg cgaagtaccg ccgggagctt cgccgggtca ccacgctgga
     2341 cgtcgagcag cccgccggcg tgccgagcgg cgcctgagcg gaccgccccc gcggccggct
     2401 cgccgaaggt ccgcgctccg cgcctgatgc gcactcgcta cgcgccccgg aaccgccggc
     2461 gcatcctctg cgtctctccg cggtacgcgc cctccttcgg caccttccac tactcctacc
     2521 cgctcttcgg cgggcgcatc cgcgccttca tgccgcccca ggggctgctg gtgatcgcgg
     2581 cctacctgcc ggagtcgtgg gaggtgcgct tcgtggacga gaactcgcgc gaggccaccg
     2641 cggaggagct cgcctgggcc gacgcgatct tcctctcggg catgcacgtg cagcggccct
     2701 tcatcgagga ggtgatcgcg cgcgcccacc gcctgggcaa gccggtggtg ctgggcggcc
     2761 cgtcggtgtc gggctgcccg gagcactacc ccggcgccga tctcatccag gtgggggaga
     2821 tgggtgactc gaccgacgcg atcatcgcgt ggctcgacgg gcacaccggg cggccgcgcg
     2881 agcagctccg cttcgacacc ggctcgcgcc tgccgctgac cgacttcccg ctccccgcct
     2941 accacctgat cgcgctgcgc cagtacttca tctcgagcat ccagttctcg agcggctgcc
     3001 cctacacgtg cgagttctgc gacatccccg cgctctacgg ccgcaatccc cgcctcaaga
     3061 ccgccgccca ggtgctggcc gagctcgacg cgctggtggc gggcggggcg gtcgccatct
     3121 acttcgtgga cgacaacttc atcgcgaacc agaaggccgc gctggagctg ctgccccacc
     3181 tggtggagtg gcagcgccgc aaccggttcc cggtgcgctt cgcctgcgag gccacgctga
     3241 acatcgcgaa gaacgagcgc gtgctcgccc tgatgcgcga cgccgggttc aacgtggtgt
     3301 tctgcgggat cgagacgccc gagcccgagg cgctccgctc gatgtcgaag gaccagaacc
     3361 tccgcatgcc gatcctggag gcggtccgca agctgaacga ctacgggctc gaggtggtct
     3421 ccggcatcat cctgggcctc gacaccgata ccgaggcgac cgcggatcac ctgatcgaat
     3481 tcatcgaggc ctccgagatc ccgatgctca cgatcaatat cctctacgcc ctgcccaaga
     3541 cgccgctgtg ggaccggctg gccgcggagg gccggattct cccagacggc agtcgcgagt
     3601 ccaacgtgat cttccggctg cccgaggaga cggtgctcgc catgtggcgg cgctgcatcg
     3661 cggccgccta cgcgccggag gcgatctacc gccggttcgc ccacaacctc gcccacacct
     3721 tcacgaagcg cgcggcctac ccgcggagct cccaccgggc ctcctggcgc aacgtcgtgt
     3781 cgggtctggc cctgctcggg cgcatcgtgt ggcggatcgg cgtccgcggc gactaccgcc
     3841 ggaccttctg gcgggtcgcc ggccctgcgc tccgggccgg gcaggtcgag gagatgatcc
     3901 aggccgcggt ggtcagccac cacctgatcg agttcacgcg ccagtgcctg cgcggggccc
     3961 aggaggcctc gttctacgcg cccagcctcc cacgcccgcc cgcggagccg cccacgctcc
     4021 atcccatccg cctgcggcgc gaggaagtct cgagctaggg atcaaccacc aggagccccc
     4081 catgcccaca cccgatcagc gcgcgagcgt cgtccggaag gatctcgaca acgtcatcca
     4141 cccgatcgtg ccccacaagc agctcgagca gcagcagctc gtcatcgtct ccgccaagga
     4201 ctcgacggtg gtggacgcgg acggccgcga gtacctcgac ggcatggccg ggctctggtg
     4261 cgtcaacatc ggctacgggc ggaccgagct ggccgacgtc gccgcggagc agatgcgcca
     4321 gctctcgtac tacccgcaca ccgcgatgaa cctgcccgcg gccgcgctgg ccgagaaggt
     4381 caacgggctg ctgggcggcg acaaccacgt gtacttcgtc agctcgggct cggaggccaa
     4441 cgaggccgcg ttcaagttcg cacgccagta cgcccgccac gagtacccgc agcagaatcg
     4501 ctaca
//
LOCUS       DASUFA010000030         7961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_121439, whole genome shotgun sequence.
ACCESSION   DASUFA010000030 DASUFA010000000
VERSION     DASUFA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121439"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="VFX87_01035"
     CDS             complement(<1..1886)
                     /locus_tag="VFX87_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4993514.1"
                     /translation="MRAATPASRAKSPKRRAPSAVGLEQRLAEALGQQAATSEILRVI
                     SSSPNDAQPVFDAIVASAARLCEAGFSAVARLDDDGLLHLAAINNMSPAETRAYRSLF
                     PRPAHRRFIMGRAFVDGRAVHVDDVLEDPDYDPHTLEVLQRAAPYRSFLALPILRHGV
                     PIGVIGCGRREVRPFTAAQIELVKTFADQAVIAIENTRLFNELGARNRELTETLEQQT
                     ATSEILRVISGSPTDVQPVFDAIVRSAVRLCGALMSCVFRFDGDLIHFVGHHNFSPGG
                     LAVFQRTYPLPLAQDKVIGQAILRREPVNVSDVLIEFRSPIGQEELGYRSVLAVPMLH
                     DGKPIGVIATSRVEIGRFPDAHVELLTTFADQAVIAIENVRLFRALEVRNRDLTEALE
                     QQTATNEILSVISSSPTDVQPVFDTIVHSAVRLCDGLFSALFRFDGELIHQVAQHNFT
                     REGLEEVRRIYPARPSRAHGSGRAILERAVVHIQDVELDPEYLHQNLTRAIGLRSGLY
                     VPMQRDGEPIGVVMVARAEPGPFSDAQIELLRTFAAQAVIAIENVRLFNELETRNREL
                     TETLEQQTATSEILRVISSSPTDVRPVFDTIASNARRLCRADSGAVYTYDGGLLHLQS
                     LDNT"
     gene            complement(2006..5587)
                     /locus_tag="VFX87_01040"
     CDS             complement(2006..5587)
                     /locus_tag="VFX87_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4993515.1"
                     /translation="MTGGAGSRYREAWIGLTVRCPQCRQENPSEAKFCLECGHRLPAG
                     CEACGRPLPVGARFCMECGAPAGATARAEPPGAPEAYTPRHLAEKILATRTALEGERK
                     SVTVLFCDVVRSTTLAEELGAEGMHTLLSRFFETALAEVHRYEGTINQFLGDGFMALF
                     GAPIAHEDHARRAALAALGIRRTLEERPLEIAPGRPVPLRLRLGLHTGFVVVGAIGDN
                     LRMDYTAVGDTTHLAARLQQMAEPGGILASEATARLVRGYVGLEPRGAVAVRGLSAPV
                     SIHAVTGPGERRSPLEAPGERPLSHFVGRDRELRSLRDLLAEVEAGRGQVVGIVGEPG
                     VGKSRLVLELQHALAGRPSLYLEGRCLSFGGAIPYVPVHDIVRALCQVSDADPPEAAR
                     IAALGMFAEIGLDATATPFLLGLLGVKTADDLPAGLSPEAIQARTFAAFRQAIIRASR
                     RRPIVLAVEDLHWIDRTSEECLASLVESLAGVPVMLLTTYRPGYRPSWLDRSYATQLS
                     LARLQPADSLSVVRSILPQAAPGDPLAKLILDKAEGNPFFLEELARAVDDHDLARPGL
                     TVPDTVHGVLAARIDRLAELPKRLLQTASVLGREFSLRLLVAVWEGDPPEAHLRELTR
                     QEFLYERSGAEEPTFVFKHALTQDVALASILAPRRREVHARAAAALGALYPSRQAELE
                     PLLAHHYFHAEAWTPACEHATRAAENARAAFANREALERYDQALVAGERAGLAPAQRL
                     ALLTARGRVHGALGAFDSARADLEAALAVARDIADAGACAALLGELGELWGGHRDYPR
                     GLELTQEAVRTAEAAGDRRATAEALLRTGLMHLNLAQIAQSQRELERALAIFEQLSDE
                     RGSARTIDVLAMTDGLTARIERCIEREREALRRFQRLGDRPAQPSVISNIGFWLAFAG
                     RRAEALPLVRQGLQSAIELGARADEAYAHVSTGWVLEQFGALGAALRESLTALELARR
                     IGHREWTAVALAMSGRITRVCGAPTRARLMHDEMLATTRELGTALWISSALAELGEDL
                     IALGQADEGERSLHEAIATAGEATQFVLPALLALSELKLGQGLPTAALELARRAEDAA
                     GDYVAWRLHARRLCGDVWIAQGREEQGEALLRAIRASAATAGLDPVVWRTGLDLAALL
                     DRRRQAAEAAGLRAEVRAALDGVAADLPADLQAAFAAGSLMRRAQGD"
     gene            complement(5602..5904)
                     /locus_tag="VFX87_01045"
     CDS             complement(5602..5904)
                     /locus_tag="VFX87_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4993516.1"
                     /translation="MYGTLRGALAGRKIAFDRDGYTATVAGRIVGVGKTIRIKSIHMR
                     YELAVPAESREATERALALHPQGCPAHQSVQGAIDVTWEARVRVGDEVVTFRSQAE"
     gene            complement(5905..6099)
                     /locus_tag="VFX87_01050"
     CDS             complement(5905..6099)
                     /locus_tag="VFX87_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993517.1"
                     /translation="MPDDFEYVSQSRLEYRHGYRHAYLGEVPAPVVYGVQGKLREYYG
                     VAEGPPVASTLDHIVAAVAG"
     gene            6228..6656
                     /locus_tag="VFX87_01055"
     CDS             6228..6656
                     /locus_tag="VFX87_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX4993518.1"
                     /translation="MSVEDNKALVRRVLAEVRDGWTQAVIDQFFAPDYRRHLTPAGPH
                     LTREEQRERASRLRAAFPDADAALEDMVAEGDRVAYRLTIRGTHRGSFLSVAATGRRV
                     MVSFFAIVRVENGKLAEEWGGLDQVDLLRQLREPRESPAP"
     gene            6719..7480
                     /locus_tag="VFX87_01060"
     CDS             6719..7480
                     /locus_tag="VFX87_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX4993519.1"
                     /translation="MAGAERAVGLAIMAKAPRVGAVKTRICPPLRAPEAAELARCFLL
                     DTVDRVRMVAGARPIMAYAPVEAQSQFEEAASGFVLIPQRGGDLGERQRHLIEDVLAL
                     GLQAALVIGTDSPTLPRECLDEAVSLVMAPDVDVVLGPTEDGGYYLIGMRALCPALFE
                     NMPWSMPTVLGRMLGRAQRLGLRVACLPTWFDVDTGADLERLRVELEASPGAAPRHTR
                     AFLERRIVCEVSVVKPANHEHHTPDGGGAAASRGR"
     gene            7428..>7961
                     /locus_tag="VFX87_01065"
     CDS             7428..>7961
                     /locus_tag="VFX87_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX4993520.1"
                     /translation="MNTTHRTAEEQRLHAAGDRSAYWKRWGPYLSERQWGTIREDYSP
                     HGTAWDSFPHDHARSRAYRWGEDGLAGISDNHQRLCFAVALWNGRDPILKERLFGLTG
                     NEGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAECPHARLVEENRRRGRRATEFEVL
                     HTGVFDDSRYFDVPVEYA"
BASE COUNT         1144 a         2747 c         2955 g         1115 t
ORIGIN      
        1 ctcgtgttgt cgagagactg caggtggagc aatccgccgt cgtacgtgta gacggcaccg
       61 gagtcggccc ggcacagtcg ccgcgcattg ctcgcgatcg tgtcgaagac gggccggacg
      121 tcggtcggcg agctcgagat gacgcgcagg atctcgctcg tcgcggtctg ctgctccagg
      181 gtctcggtca gctcgcggtt ccgcgtctcc agctcgttga acaggcggac gttctcgatc
      241 gcgatgaccg cctgggccgc gaaggtcctc aggagctcga tctgggcatc cgagaacggc
      301 cccggctcgg cgcgggccac catgaccacg ccgatgggtt cgccgtccct ctgcatcggg
      361 acgtagagcc cgctgcgcaa cccgatcgcg cgggtcaggt tctgatgcag atactccgga
      421 tcgagctcca cgtcctggat gtggacgacc gcgcgctcca ggatggcccg accggacccg
      481 tgagcccgac tcgggcgcgc ggggtagatc cggcgcactt cctcgagccc ctcgcgggtg
      541 aagttgtgct gggcgacctg gtggatcagc tcgccgtcga accggaagag cgcgctgaac
      601 aggccgtcgc acagccggac cgcgctgtgg acgatcgtgt cgaacacggg ttggacgtcg
      661 gtcggggagc tggagatgac gctcaggatc tcgttcgtcg cggtttgctg ctcgagcgcc
      721 tcggtcaggt cacgatttcg cacctcgagc gcccggaaga ggcggacgtt ctcgatcgcg
      781 atgaccgcct ggtcggcgaa ggtcgtgagg agctcgacgt gggcgtccgg gaaccggccg
      841 atctcgacgc gcgaggtggc gatcacgccg atgggcttgc cgtcgtggag catcgggacc
      901 gcgaggacgc tgcgatagcc cagctcttcc tgtccgatcg gggaccggaa ctcgatcagg
      961 acgtcggaga cgttgacagg ctcgcggcgc aggatagcct gaccgatcac cttgtcctgg
     1021 gcgaggggca ggggataggt ccgctggaac acggccaggc cgccgggtga gaagttgtgg
     1081 tgaccgacga agtggatgag gtccccgtcg aaccggaaga cgcagctcat gagggcgccg
     1141 cacaatctga ccgcgcttcg gacgatcgcg tcgaacaccg gctggacgtc ggtcggcgag
     1201 ccggagatga cccggagaat ctcgctcgtc gcggtctgct gctcgagggt ttccgtcagc
     1261 tcacgattgc gcgcgcccag ctcgttgaac aagcgggtgt tctcgatcgc gatgaccgcc
     1321 tggtcggcga aggtcttcac gagctcgatc tgggcggccg tgaaggggcg gacttcgcgg
     1381 cggccgcagc cgatcacgcc gatcggcacc ccatgccgga ggatcgggag ggccaggaag
     1441 ctccggtagg gcgccgcgcg ctggagcacc tccagggtgt gcgggtcgta gtcgggatcc
     1501 tccagcacgt cgtcgacatg gaccgcccgg ccgtccacga aggcgcggcc catgatgaac
     1561 cggcggtgcg cggggcgcgg gaagaggctg cgataggccc gggtctcggc gggcgacatg
     1621 ttgttgatcg ccgcgaggtg gagcagcccg tcgtcatcga gccgcgccac cgcgctgaat
     1681 cccgcctcgc acagccgggc cgcgctggcg acgatggcat cgaacacggg ctgggcgtcg
     1741 ttcggcgagc tcgagatgac ccgcaggatc tcgctggtag ccgcctgctg cccgagcgcc
     1801 tcggcgaggc gctgctcgag accgacggcg gagggggctc gacgcttggg cgacttcgcg
     1861 cgactggcgg gcgtcgccgc cctcaccttg gccttagcgg acttcggctt ccgacggata
     1921 gccttctcct gcccgggtta ccggcgcctt ggatcgggcc ggggatcgaa cgacaggcta
     1981 agcgaaggcg gccgccgggg caagtctagt cgccctgagc ccggcgcatc agcgagccgg
     2041 cggcgaaggc ggcctgcagg tccgcgggca ggtcggcggc gacaccgtcg agcgcggccc
     2101 gcacttcggc ccgcaggccg gcggcctccg cggcctgcct gcgacggtcg agcagagccg
     2161 ccaggtcgag gccggtgcgc cataccaccg ggtccaggcc ggccgtcgcg gcgctggccc
     2221 ggatggcccg caggagggcc tcgccttgct cctcgcgccc ctgcgcgatc cagacatccc
     2281 cgcagagccg ccgggcgtgg agcctccagg ccacatagtc tcccgccgcg tcctcggcgc
     2341 gccgggccag ctcgagcgcc gccgtcggga ggccctggcc gagcttgagc tccgagaggg
     2401 ccagcagcgc gggcaggacg aactgggtgg cctctccggc cgtggcgatc gcctcgtgga
     2461 gagaccgctc gccctcgtcc gcctggccca gggcgatcag gtcctcgccc agctcggcca
     2521 gtgcactcga gatccacagc gcggtgccga gctcgcgcgt ggtcgccagc atctcgtcgt
     2581 gcatgagccg ggcgcgggtc ggcgcgccgc agacgcgcgt gatccggccc gacatggcga
     2641 gggccaccgc cgtccactcg cggtgtccga tccggcgcgc cagctcgagg gcggtcaggc
     2701 tctcgcgcag agcggcgccg agagcgccga actgctccag tacccagccg gtgctcacgt
     2761 gcgcgtaggc ctcgtccgcc cgcgcgccca gctcgatcgc cgactggaga ccctgccgca
     2821 cgagagggag ggcctcggcg cgccggcccg cgaaggcgag ccagaacccg atattcgaga
     2881 tcaccgaggg ctgggccggc cggtcgccga gccgctggaa gcggcggagc gcttcgcgct
     2941 cgcgctcgat gcaccgctcg atccgggccg tcaggccgtc ggtcatcgcg agcacgtcga
     3001 tggtccgcgc gctgccgcgc tcgtccgata gctgctcgaa gatggcgagc gcccgctcca
     3061 gctcgcgctg gctctgggcg atctgcgcca ggttgaggtg catcagcccg gtgcggagga
     3121 gggcctccgc ggttgcccgc cggtcgccgg cggcctcagc ggtccggacc gcctcctgcg
     3181 tgagctccag gccgcgcggg tagtcgcggt ggccccccca cagctcgccg agctccccca
     3241 gcagtgcggc gcaggcgccc gcgtcggcga tatcccgggc gacggcgagc gccgcttcga
     3301 gatccgcgcg cgcggagtcg aacgcgccga gggcaccatg gacccggccc cgcgcggtga
     3361 gcagggcgag gcgctgggcc ggggccagcc cggcccgctc accggccacg agcgcctggt
     3421 cgtagcgctc gagcgcctcg cggttggcga aggcggcgcg ggcgttctcg gcggcacggg
     3481 ttgcgtgctc acacgccggc gtccaggcct ccgcgtggaa gtagtgatgg gcgagcaggg
     3541 gctcgagctc ggcctgacgg ctcgggtaga gcgcgccgag cgcagcggcg gcgcgcgcgt
     3601 gcacctcgcg acggcgcggc gccaggatgc tggccagggc cacgtcctgg gtgagcgcgt
     3661 gcttgaagac gaacgtgggc tcctcggcgc ccgagcgctc gtagagaaac tcctggcggg
     3721 tcagctcgcg caggtgcgcc tcgggcgggt cgccctccca gaccgcgacg agcagccgca
     3781 gggagaactc gcgcccgagg acggaggcgg tctggagcag gcgcttcggc agctccgcga
     3841 gccggtcgat gcgcgcggcg agcacgccgt gcaccgtgtc cggcacggtg agccccggac
     3901 gcgccaggtc gtgatcgtcc accgcgcgcg ccagctcctc gagaaagaac gggttgcctt
     3961 cggccttgtc gaggatcagc ttcgcgaggg gatcaccggg cgcggcctgg ggcaggatgg
     4021 agcgcaccac gctcaggctg tccgccggct gcagccgggc gagcgagagc tgcgtcgcgt
     4081 aggagcgatc gagccacgac gggcggtagc ccggccggta cgtggtcagc agcatcaccg
     4141 ggacgcccgc caggctctcc acgagcgagg ccaggcactc ctccgaggtg cggtcgatcc
     4201 agtggagatc ctcgacggcc aggacgatgg gccgccgccg gctcgcacgg atgatcgcct
     4261 gccggaaggc cgcgaaggtg cgcgcctgga tcgcctccgg gctcaggccg gccgggagat
     4321 catccgccgt tttcaccccg agcaggccga gcaggaacgg ggtcgccgtc gcgtccagcc
     4381 cgatctcggc gaacatgccc agcgcggcga tgcgcgcggc ctcgggcgga tcggcgtcgg
     4441 agacctggca gagcgcccgg acgatgtcgt ggaccggcac gtacgggatg gcgccgccga
     4501 aggacaggca gcggccctcg agatagagcg agggcctccc ggcgagggcg tgctgcagct
     4561 cgaggaccag gcgtgatttt ccgaccccgg gctcgcccac gatccccacc acctgcccgc
     4621 gcccggcctc gacctcggcg aggagatcgc gcaggctccg tagctcgcgg tcgcgcccca
     4681 cgaagtggct gagcgggcgc tcgccggggg cctcgagcgg ggaccggcgc tcgcccgggc
     4741 ccgtcaccgc gtggatggac acgggcgcgc tcaggccccg gaccgcgacg gcgccgcggg
     4801 gctcgaggcc cacgtagccg cgcacgagcc gggcggtggc ctcgctcgcc aggatgccgc
     4861 cgggctcggc catctgctgc aggcgcgcgg ccaggtgggt ggtgtcgccg accgcggtgt
     4921 agtccatgcg gaggttgtcg ccgatggcgc ccacgaccac gaagccggtg tggagcccca
     4981 ggcggaggcg gagcggcacc ggtcgccccg gagcgatctc gagaggccgc tcctcgagcg
     5041 tgcgccggat gccgagggcc gccagcgccg cgcggcgcgc atggtcctcg tgggcgatcg
     5101 gcgcgccgaa gagggccatg aacccgtcgc cgaggaactg gttgatcgtg ccttcgtagc
     5161 ggtggacctc ggcgagcgcc gtctcgaaga agcggctgag cagcgtgtgc atgccctcgg
     5221 cgccgagctc ctcggccagc gtggtcgagc ggaccacgtc gcagaagagc acggtcaccg
     5281 acttgcgctc accttcgagc gcggtgcggg tggcgaggat cttctcggcc aggtgacgcg
     5341 gagtgtaggc ctcgggagcg ccgggcggct cggcgcgcgc ggtggcccct gcgggcgcgc
     5401 cgcactccat gcagaacctc gcacctacgg gcaggggccg gccgcaggcc tcgcagcccg
     5461 cgggcagacg gtggccgcac tcgagacaga acttcgcctc ggacggattc tcctgccgac
     5521 actgtggaca gcgcaccgtg agcccgatcc aggcctcacg gtagcggctg cccgcgccgc
     5581 cggtcaagcc ttggagtcgg tctactcggc ctggctcctg aacgtcacga cctcgtcacc
     5641 gacccggacc cgggcctccc aggtcacgtc gatggcgccc tgcacgctct ggtgcgccgg
     5701 gcagccctgc gggtgcaggg ccagcgcccg ctcggtggcc tcgcggctct ccgcgggcac
     5761 cgcgagctcg tagcgcatgt ggatggactt gatccggatg gtcttgccga ccccgacgat
     5821 gcggcctgcc acggtggccg tgtagccgtc gcggtcgaac gcgatcttgc gccctgccag
     5881 ggcgccgcgc agcgtgccgt acattcagcc ggcgacggcc gcgacgatgt ggtcgagggt
     5941 ggaggcgacc ggcgggccct cggcgacgcc gtagtactcg cgcagcttgc cttgcacgcc
     6001 gtagaccacc ggggcgggca cctcgccgag ataggcgtgg cggtagccgt gccggtactc
     6061 gaggcgggac tgggacacgt attcgaagtc gtcgggcatg gagacctcct tgggttgatt
     6121 ggacgcagcc aaccttatca gccaaaggag aatggagatg aacaagagtc ggccgaccgg
     6181 cttgaggtgg gcgtggaaca tgcttagcat gcggccggag gtgtgccatg tcggtcgaag
     6241 acaacaaggc cctggtgcgt cgggtcctgg cggaggtgag agatgggtgg acccaggcgg
     6301 tgatcgacca gttcttcgcg ccggactacc gccgccatct cacgccggcg ggtccgcatc
     6361 tcactcgtga ggagcagcga gagcgggcct cgcggctgcg ggccgccttt cccgacgccg
     6421 acgccgcgct ggaggacatg gtggccgaag gagaccgcgt ggcgtacagg ttgaccattc
     6481 gtggaaccca tcggggctct ttcctgagcg tggcagcgac cggccggcgc gtgatggtct
     6541 cgttcttcgc gatcgtccgc gtcgagaatg gcaagctggc cgaggagtgg ggcggcctcg
     6601 accaggtgga tctactccgg cagctccgag agccgaggga atcgccggcg ccctgagccg
     6661 catccaggtg acgaatgtaa gacctgaccc cgggcatacc acatggcatg atggctccat
     6721 ggcgggggcg gagcgggcgg tggggctggc gatcatggcc aaggccccgc gggtgggggc
     6781 ggtgaagacg cggatctgcc cgcccctgcg ggccccggag gcggccgagc tggcgcggtg
     6841 tttcctcctg gacaccgtgg atcgggtgcg gatggtggcc ggggcccggc cgatcatggc
     6901 gtacgcaccg gtcgaggcgc agagccagtt cgaggaggcc gcatccggct tcgtgctgat
     6961 cccgcagcgc ggcggggacc tgggcgagcg ccagcgccac ctgatcgagg acgttctcgc
     7021 gttgggcctc caggcggcgc tggtgatcgg cacggactcc ccgacgctgc cgcgggaatg
     7081 cctggacgag gcggtcagcc tcgtcatggc ccccgatgtg gacgtggtcc tcggccccac
     7141 cgaggacggt ggctactacc tcatcggcat gcgcgcgctc tgtcccgccc tgttcgagaa
     7201 catgccctgg agcatgccca ccgtgctcgg ccgcatgctc gggcgggccc agcgcctggg
     7261 cctccgggtg gcctgcctgc ccacgtggtt cgacgtggac accggcgccg atctcgagcg
     7321 cctgcgcgtc gagctcgagg cgtcgcccgg cgccgcgcca cgtcacaccc gggcgtttct
     7381 cgaacgccga atcgtttgcg aggtctccgt cgtcaaaccc gccaatcatg aacaccacac
     7441 accggacggc ggaggagcag cggcttcacg cggcaggtga tcgctcggcc tactggaagc
     7501 gctgggggcc gtatctcagc gagcgccagt ggggcacgat acgcgaggac tacagccccc
     7561 acggtaccgc ctgggactcc ttccctcacg atcacgcccg ctcccgggcc tatcgctggg
     7621 gggaggacgg gctggccggc atctccgaca accaccagcg gctctgcttc gcggtggcgc
     7681 tgtggaacgg ccgcgacccc atcctgaagg agcggctctt cgggctcacc ggcaacgagg
     7741 gcaaccacgg cgaggacgtc aaggaatact acttctacct cgactccacg ccgacgcact
     7801 cgtacatgaa gtacctctac aagtacccgc aagcggagtg cccccatgct cgcctcgtcg
     7861 aggaaaaccg ccgccgcggg cgccgcgcca ccgagttcga ggtgctgcac accggtgtct
     7921 tcgacgacag ccgttacttc gacgtcccgg tcgagtacgc c
//
LOCUS       DASUFA010000031         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_122005, whole genome shotgun sequence.
ACCESSION   DASUFA010000031 DASUFA010000000
VERSION     DASUFA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122005"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="VFX87_01070"
     CDS             complement(<1..1910)
                     /locus_tag="VFX87_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4993521.1"
                     /translation="MTVTLALVLAAAGILVTVQHMGFKTSGRDVLPQDAGYVKKYVEY
                     ARDFGELEDIVVVVEARTFDAAKAYASRLVQELRASPVKFPRLSYRIDPKRFEGRQLL
                     YLSTEKLREIRDKIFDHQEFMEGFANDPSLARLLEGVNVQFAQAFLNNVFDLGIADEG
                     PADTRFLQVLLEQIDQRLDRPTPYRSPWANLFSFGKDADPDAGYFLSDDKSLLFVLVE
                     TPKGDKSSFAGDARAIEVIRDAVTRLRAVFPSVQAGVTGAPVLSNDEMSAAFQDSQVA
                     TVLSFVLTLGIMWLAYWRLGKPVLMLIVLAVSIAWSLGVITLTLGHLTIFSVMFISIV
                     VGIGIDYGIYFLFRYEEELFLGRRLNEALEITAARAGPGMLLGALTAGGTFFVLMLTE
                     FRGIKELGYIAGLAIMLSWLAMMTVFPAVLVLVDRRHAHRPRDHQPRAHELERIHVPL
                     LEQVTRYPVTVLTVAGAVTAASLWALPSVAFDYNLLNLQAKGTESVTWEKRILATTGR
                     SGFNGLASADSLEELRRLQAAFEKLPTVSEVDSVLRLIPEEQPEKVAIIRSFAPLVAP
                     VKIGRSSPVDLEHLARVVRDLKRRFDVAAAEGGAKLPAEIRKLRDMSGALLARLEKID
                     REVAEPALTHLQS"
     gene            complement(2034..3122)
                     /locus_tag="VFX87_01075"
     CDS             complement(2034..3122)
                     /locus_tag="VFX87_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX4993522.1"
                     /translation="MGEARSEAVRVAIVGVGNCASALVQGVHYYRNAPDDGFIPGLMR
                     PRLGPYHVGDIEFSAAFDIDARKVGADLSEAIFTAPNNTVRFAEVPPLGVPVHRGMTH
                     DGLGHYLSQVITKAPGSTADIAGILRETRTDVVVNFLPVGSEMATKWYVEQILDAGCG
                     FVNCIPVFIAREEYWRRRFEERGLPVVGDDVKSQVGATIVHRVLARLFMDRGVRLDRT
                     SQLNVGGNTDFYNMLERSRLESKKISKTDAVRSQLSHDLPDDSIHIGPSDYVPWLADR
                     KWAHIRLEGRGFGDQPINVELKLEVWDSPNSAGVVIDAIRCCKMAMDRGLKGALLAPS
                     AYLMKSPPEQWTDDEAHERLARFIAAAE"
     gene            complement(3200..4195)
                     /locus_tag="VFX87_01080"
     CDS             complement(3200..4195)
                     /locus_tag="VFX87_01080"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX4993523.1"
                     /translation="MRRLLRPLIVVVGVGVIGYLGHQIGFDAILDSIRTLSWRLLVVI
                     CFPYALTSTLDATAWRLAFVNHVPPFTKIWTARLAGEAVNATTPTASVGGEPVKAFLL
                     RHWVPVPEGLAAVIVDKTAIVTGQGLFLVLGLVLARTVVPVSGPVLTGMSVLLGLEAL
                     AVTGFVLVQLLGAAGRGGRLLARFGMGPNLSNQERLEGLDRALSGFYRAHRGRLVGAV
                     FVHLLAWTVGSLEIYLVLNFLGLPVTLLTAVVMESFGAAVKFASFMIPGSLGALEGGN
                     MAIFAAFGYGGAVGLSYTLVRRLRESAWAGAGLLALAALSARPTPLPTTTSEGDP"
     gene            complement(4249..>5112)
                     /locus_tag="VFX87_01085"
     CDS             complement(4249..>5112)
                     /locus_tag="VFX87_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4993524.1"
                     /translation="TETTVTESLESGAPVLRVLADTARRLAPALAAGEPLGAALMRHV
                     EATRLKQVTASGLCFCARGRADLPEADRLLYAGLGTDNDTGVDEYLHRRLSRGLTRVL
                     VRTPATPNQVSLLSLGIGLGAVWAFWNATPLSAAVGVLVYLAACVVDHSDGELARLTF
                     QESRLGAHLDWAIDTVIHSGLVLGMAISAGPGAAATVIGVVGSAGVALSALFARYLPR
                     EIAVGETVGGALKNMGNRDLFYLLLLGFVLLRWVAPAALPALALVVALGSQSYWIACV
                     DRIRRGRPAAS"
BASE COUNT          848 a         1701 c         1892 g          671 t
ORIGIN      
        1 gactgcaggt gggtgagggc gggctcggcc acctcgcggt cgatcttctc caggcgcgcg
       61 aggagggcgc cgctcatgtc ccggagcttc cggatctcgg cggggagctt ggcgccgccc
      121 tccgcagccg ccacgtcgaa ccgccgcttg aggtcgcgca ccacgcgggc gagatgctcg
      181 aggtccaccg ggctcgagcg gccgatcttc accggcgcca ccagcggggc gaagctccgg
      241 atgatggcga ccttctccgg ctgctcctcg gggatgagtc ggagcacgga atccacctcg
      301 gagacggtgg ggagcttctc gaacgccgcc tgcagccgcc gcagctcttc caggctgtcg
      361 gcggaggcga gaccgttgaa gccggagcgg ccggtggtgg cgaggatgcg cttttcccag
      421 gtcaccgatt ccgtgccctt ggcctgcagg ttcagcaagt tgtagtcgaa ggccaccgag
      481 ggcagggccc agagcgacgc ggcggtgacg gcgccggcga cggtgagcac ggtcaccgga
      541 tagcgcgtga cctgttcgag gaggggcacg tggatccgct ccagctcgtg cgcgcgcggc
      601 tggtggtccc gcggccgatg agcgtggcgc cggtccacca gcaccagcac ggcggggaat
      661 accgtcatca tggccagcca cgagagcatg atggccagtc ccgcgatgta ccccagctcc
      721 ttgatgccgc ggaactccgt gagcatgagc acgaagaagg tgccgcccgc agtcagcgcg
      781 cccagcagca tcccgggccc ggcgcgggcg gcggtgatct ccagggcctc gttgaggcgg
      841 cggccgagga agagctcctc ctcgtagcgg aagagaaagt agatcccgta gtcgatgccg
      901 atcccgacca cgatggagat gaacatcacc gagaagatgg tgaggtggcc gagggtgagg
      961 gtgatgacgc ccagcgacca ggcgatgctc accgcgagca cgatgagcat caggacgggc
     1021 ttgcccagcc gccagtaggc cagccacatg atgccgaggg tgaggacgaa cgagagcacg
     1081 gtggccacct ggctgtcctg gaaggcggcc gacatctcgt cgttggagag gacgggggcc
     1141 ccggtcacgc cggcctggac gctggggaat accgcgcgca ggcgggtcac cgcgtcccgg
     1201 atgacctcga tggccctggc gtcgccggcg aagctcgact tgtcgccctt gggtgtctcc
     1261 accagcacga agagcaggct cttgtcgtcg gaaaggaagt agccggcgtc gggatccgcg
     1321 tccttgccga aggagaagag gttggcccag ggggagcggt acggggtggg ccggtcgagc
     1381 cgctggtcga tctgctccag gagcacctgg aggaagcgcg tatccgcggg cccctcgtcg
     1441 gcgatcccga ggtcgaacac attgttcaga aaggcctggg cgaactggac gttcaccccc
     1501 tcgaggaggc gggccaggct cgggtcgttg gcgaagccct ccatgaactc ctgatggtcg
     1561 aagatcttgt cgcggatctc gcggagcttc tcggtggaga ggtagaggag ctggcggccc
     1621 tcgaagcgct tggggtcgat gcggtacgac agccgcggga acttcaccgg ggaggcgcgg
     1681 agctcctgga cgaggcggct cgcataggcc ttggcggcgt cgaaggtgcg ggcctccacc
     1741 accaccacga tgtcctcgag ctcgccgaag tcgcgcgcgt actccacgta cttcttcacg
     1801 tacccggcgt cctggggcag cacgtcgcgc ccggacgtct tgaagcccat gtgctgcacg
     1861 gtgacgagga tgccggcggc ggcgaggacg agggcgaggg tcaccgtcac cagcgggcgg
     1921 cggcaggaca cccgcacgag gcggcgaagc aggcgaccgg ttcgcgatat ggcgatcatg
     1981 gagcgcgggt gcgggcgacc ggctcagtct accatcgccc tcgggcggga gagctactcg
     2041 gcggcggcga tgaagcgcgc gaggcgctcg tgcgcctcgt cgtccgtcca ctgctcgggc
     2101 ggggacttca tgagataggc ggagggggcc aggagcgcgc ccttgaggcc acggtccatg
     2161 gccatcttgc agcagcggat ggcgtcgatg accacgcccg cggagttggg cgaatcccac
     2221 acctccagct tcagctcgac attgatgggc tgatcgccga agccccgccc ttccagtcgg
     2281 atgtgcgccc acttgcggtc ggccagccag ggcacgtagt cgctgggccc gatgtggatg
     2341 gagtcgtccg gcaggtcgtg ggagagctgg gagcgcaccg cgtccgtctt ggagatcttc
     2401 ttcgactcca gccgggagcg ctcgagcatg ttgtagaaat cggtgttgcc gcccacgttg
     2461 agctggctgg tccggtccag ccggaccccc cgatccatga agagccgggc cagcacgcgg
     2521 tgcacgatgg tggcgcccac ctgggacttc acgtcgtcac ccaccaccgg aagtccccgc
     2581 tcctcgaaac ggcggcgcca gtactcctcg cgcgcgatga acaccgggat gcagttgacg
     2641 aagccgcagc cggcgtcgag gatctgctcg acgtaccact tggtcgccat ctcgctgccc
     2701 acgggcagga agttcaccac cacgtcggtg cgagtctcgc gcaggatgcc cgcgatgtcc
     2761 gcggtggacc cgggagcctt ggtgatcacc tgggagagat agtgcccgag gccgtcgtgg
     2821 gtcatgccgc ggtggacggg cacccccagg ggagggactt ccgcgaagcg cacggtgttg
     2881 ttgggcgcgg tgaagatggc ctcggacagg tccgcgccca ccttgcgggc gtcgatgtcg
     2941 aaggcggcgg agaactcgat gtcgcccacg tggtacgggc ccaggcgcgg gcgcatgagt
     3001 ccggggatga acccgtcgtc gggcgcgttc cggtagtagt gcacgccctg gacgagcgcc
     3061 gacgcgcagt tgcccactcc cacgatcgcg acccgcactg cttccgaacg cgcctcgccc
     3121 acacgtgcct ccatgcgatg agttgttccg cgcagcgctc ccgagtctag cacaacgagc
     3181 cggcggggcg cggccggccc tagggatcac cttcactcgt ggtggtgggg aggggcgtgg
     3241 gccgcgcgga gagcgcggcg agggcgagca gcccggcgcc ggcccaggcg ctctcgcgca
     3301 gccggcgcac cagggtgtag gacagtccca ccgcgccgcc gtagccgaag gcggcgaaga
     3361 tcgccatgtt gcctccctcc agggcgccga gcgagccggg gatcatgaag ctcgcgaact
     3421 tcaccgccgc gccgaacgac tccatcacca ccgcggtgag gagggtgacc ggcaggccga
     3481 gaaagttgag cacgagatag atctcgaggc tccccaccgt ccacgcgagc aggtgcacga
     3541 acacggcgcc caccagccgg ccgcgatgcg cgcggtagaa gcccgagagc gcgcggtcca
     3601 gcccctccag ccgctcctgg ttgctcaagt tcggtcccat gccgaaccgc gcgaggagcc
     3661 ggcccccgcg cccggccgcc ccgaggagct ggacgaggac gaagccggtc accgcgaggg
     3721 cctcgagacc caggagaacg gacatgccgg tgagcacggg tcccgacacc ggcaccacgg
     3781 tgcgcgcgag aacgagcccc agcacgagga agaggccctg gccggtgacg atggcggtct
     3841 tgtccacgat gacggcggcc aggccctcgg gcacgggcac ccagtggcgg aggaggaacg
     3901 ccttgacggg ctcaccgccc acggaggcgg tgggcgtggt cgcgttcacc gcctcgccgg
     3961 cgagccgcgc ggtccagatc ttggtgaagg gcggcacgtg gttgacgaag gcgaggcgcc
     4021 aggcggtggc gtcaagcgtg gaggtgagcg cgtagggaaa gcagatcacc acgagcagcc
     4081 gccacgacag cgtccggatg gagtccagga tggcgtcgaa gccgatctgg tgaccgagat
     4141 agccgatcac gccgacgccc accaccacga tgaggggacg gagcagacgg cgcacggcgg
     4201 cgcgcggacg cgtgacgggc gcggccggga tggggcccgc cccgcccgct aggacgcggc
     4261 cggacggccg cgccggatcc gatccacgca cgcgatccag tacgactgcg agcccagggc
     4321 caccaccagc gccagcgcgg gcagcgccgc gggcgccacc cagcgaagca ggacgaagcc
     4381 caggagcaga aggtagaaga gatcgcggtt gcccatgttc ttgagggcgc cgcccaccgt
     4441 ctcgcccacc gcgatctcgc gcggcaggta gcgcgcgaag agcgcgctga gcgccacgcc
     4501 cgccgatccc accactccga tgaccgtcgc ggcggcgccc gggcccgcgg agatcgccat
     4561 gcccagcacc agacccgagt ggatcacggt gtcgatggcc cagtcgaggt gggcgccgag
     4621 ccgggactcc tggaaggtga ggcgggcgag ctcgccgtcc gagtggtcca ccacgcaggc
     4681 ggccagatag accagcacgc ccaccgcggc cgacagcggg gtggcgttcc agaaggccca
     4741 caccgcgccc aggccgatgc cgaggctcag gagactcacc tgattgggcg tggccggagt
     4801 acggaccagg acgcgcgtga gtccgcgcga gaggcgccgg tgcaggtact cgtcgacgcc
     4861 ggtgtcattg tcggtgccga gtccggcata gagcagccgg tcggcctccg ggagatcggc
     4921 gcggccgcgc gcgcagaagc agagacccga cgcggtgacc tgcttgagcc gcgtcgcctc
     4981 cacgtggcgc atgagcgccg cccccagggg ctcgccggcg gccagcgcgg gggcgagccg
     5041 gcgcgcggtg tccgccagca cgcggaggac gggggctccg gactcgagcg actccgtcac
     5101 cgtggtctcg gt
//
LOCUS       DASUFA010000032        14608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1607282, whole genome shotgun sequence.
ACCESSION   DASUFA010000032 DASUFA010000000
VERSION     DASUFA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14608
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607282"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFX87_01090"
     CDS             complement(<1..618)
                     /locus_tag="VFX87_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4993525.1"
                     /translation="MAGKMVDFPSNGHSAKGYLATPASGKGPGVLVIQEWWGLVGHIK
                     NVCDRFAAAGFSALAPDLYHGQTATEPDAAGKLFMALNIQRAEKDLSGAAKFLAGHSS
                     TAKLGAVGFCMGGQLALFAGCTNVNVGAVVDFYGIHPNVKPDYRKLNGPVLGLFAEKD
                     LKGATAYLAQHSSTPKLGAVGFCMGGQLALFAATLNPAIGATVNFY"
     gene            complement(838..2100)
                     /locus_tag="VFX87_01095"
     CDS             complement(838..2100)
                     /locus_tag="VFX87_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX4993526.1"
                     /translation="MIYVWLIGLSLLVTALFSAAEMSFIAANRLRLRHMAEGGHRTAI
                     RYLEAFRSPERLLSTAMMGVTIAHITASSVATWALIPVVGGGAALLATAALTPLMLVF
                     GEVIPKAVARERATGLILILFPVVELLGKVLSPLTWGANALVGRALALVGRGPTGTRQ
                     FVSREELKLLLQMEPEEADVTVTEAEMIDKIFDLGETAVREVMVPLIDVAALPETASP
                     ADAIRLIQERGFSRIPVFTDRAFNIVGVVTTMDLFSRGVQGPDVRSLMRPATYVPETK
                     RIDELLREMQKNRVQLAVVVDEYGGAVGIVTVEDIVEEIVGEIRDEHDRTPDTVERLP
                     DGSYRVAGRAGLDEVNEALDWDLPKGDFETVAGLVLATLHRIPLVGEVFRVGRYSFTV
                     LEADKRRVLTVRVTPGPAKKPAPDKPES"
     gene            complement(2097..3377)
                     /locus_tag="VFX87_01100"
     CDS             complement(2097..3377)
                     /locus_tag="VFX87_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX4993527.1"
                     /translation="MDSRVFPELIVLGILLLVSALLSGAEAAYFSLGRTRLKELAEQQ
                     GKTPGPLAPLLAQPNELLVTLLVGITVVNIGASALAAAIAERIFGGKGLPIAIGAMVF
                     LLTVFGEVLPMTLAVEHPLRFASWVNRPVAWLSVLLKPIRIGLGAFTAVTLRLVGSER
                     KVGQPEISEEELRTLVDVGAREGVVERTEREMIHKVFELEDTLVREVMVPRPDMFCLD
                     MSTPSGEILPLLREQLHSRVPVFEETIDQIVGVLYTKDLLPHLGGLPPAFELRAHLHP
                     PYFVPESKRADALLREFQAKKLHLAIVVDEYGGTAGLVTLEDLLEELVGEIRDEYDED
                     ERLIQGVGPRTFRVSGRLPIHELNATTGLAIPDESFDTVGGWVLDLFGRVPHRGEKKE
                     TEDGVRVSVEKVERTRVLEVLVALPEVAGPPEAT"
     gene            complement(3370..4422)
                     /gene="thiL"
                     /locus_tag="VFX87_01105"
     CDS             complement(3370..4422)
                     /gene="thiL"
                     /locus_tag="VFX87_01105"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX4993528.1"
                     /translation="MNAEPPERVSLGGLGELGLIRRIRSRAAASEAAAGVEVGIGDDA
                     AVLTVPPGHKLLATTDLLIEDVHFRRISAAPADIGWKAMAVNLSDIAAMGGIPRWALV
                     ALAVPADTDVEAVDTFYAGMAEAAAPHGVTVVGGDTSAAPGGWMVNVTLLGTHPGEPR
                     LRSHARAGDAVALTGSLGASAAGLHALEIGLDRARDARLAGGALAEITRAHLRPRARV
                     AEGRWLGQAPGVRAMMDCSDGLATDLGHICRESGVGARVRLERLPVAAAAREAARALG
                     GDAWEWAVSGGEDYELLLTCDPAAADRLIAGLAEATGTALTVIGRIEGTTGEIVFVDA
                     DGAPVTMRGGFEHFHG"
     gene            complement(4419..5003)
                     /locus_tag="VFX87_01110"
     CDS             complement(4419..5003)
                     /locus_tag="VFX87_01110"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX4993529.1"
                     /translation="MILVLDNYDSFTYNLVQYLGELGARIRVARNDALTSDDVEALVP
                     EGIVISPGPGSPDGAGISLELIRRFYARVPILGVCLGHQAIGQAFGGTVARARRQMHG
                     KTSPITHDGRGVFRGLPPRFEATRYHSLVVLEAGLPTDLEITARADDGEIMGLRHRHY
                     PVEGVQFHPESILTSQGKALLTNFLALTPVTAPA"
     gene            complement(5000..6538)
                     /gene="trpE"
                     /locus_tag="VFX87_01115"
     CDS             complement(5000..6538)
                     /gene="trpE"
                     /locus_tag="VFX87_01115"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX4993530.1"
                     /translation="MSTVGPGLPPLSPTREEFRALAGQGNLIPVFAELAADLDTPLSA
                     FLRLRPGPYAFLLESVEGGEKWARYSFLGTDPLMVFTAKGKRISVRRADGTIELLAGS
                     DPFKALRDLLAQFKPVPVPGLPRFQGGAVGFLSYDMIRHVERLPTLTTDDQKLPDAVF
                     MFTDSLLVFDNLRHRLLVIANAHITKTDPASLDRAYDAAAVKIGMLLGNLRRPARSPA
                     PLTLPPVEPLMALGDEGFTSTMDEAAFTDGVRRAKEFIAAGDAYQVVLSRRLDTELRA
                     DPFTVYRALRTVNPSPYLFFLRLGKTSIVGSSPEVLVRLEDERVEERPIAGTHPRGRT
                     EQEDARLADEMRADPKERAEHVMLVDLGRNDVGRVSRLGSVEVSEFMVVERYSHVMHL
                     VSHVRGRLESGKDAFDVLAACFPAGTLTGAPKIRAMEIIEELEPTRRGPYGGAVGYIS
                     YSGNLDSCITIRTVVCHGRRASIQVGAGIVADSDPKTEWLETASKARGMILALRIAAQ
                     EQPA"
     gene            complement(6550..6876)
                     /gene="trxA"
                     /locus_tag="VFX87_01120"
     CDS             complement(6550..6876)
                     /gene="trxA"
                     /locus_tag="VFX87_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX4993531.1"
                     /translation="MADSTLHLTEASFDAELDKHTELLMVDFWAEWCAPCRAIAPTLE
                     ELVREGNGAVTLAKVNVDEQPALAARYGIRSIPTILFVKGGKVMDQVIGAVPKAQLKK
                     KLDANA"
     gene            7327..7677
                     /locus_tag="VFX87_01125"
     CDS             7327..7677
                     /locus_tag="VFX87_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993532.1"
                     /translation="MVVDDAVSELKLIEGILKAAGHQVVSYDHGELLEDRVVEERPDL
                     LLLDIVMPKRNGYEVLRGLRRDPRTKDTPVVFVSSKNQESDRLWGQRQGAEDYLAKPF
                     TADQLLGVVRQFVK"
     gene            7683..8198
                     /locus_tag="VFX87_01130"
     CDS             7683..8198
                     /locus_tag="VFX87_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4993533.1"
                     /translation="MTQPPAIPVLPAAPDAPPAPRACLFALAGARFAVDVRSAREVVL
                     FEEITVVPRAPRHLVGVANLRGTVMPIVDVRELLGLPASRPGRSLRTLVVRDGAVQAA
                     LVVDTVLGLEPFEAVLPADNSTAAGMGGPRRLMTGWIQWAGETLPLLDVSKILAALRP
                     PTPTVAQEASA"
     gene            8195..9445
                     /locus_tag="VFX87_01135"
     CDS             8195..9445
                     /locus_tag="VFX87_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993534.1"
                     /translation="MNRREFLGGVLSTGLASTLLPGGGARAQAGEIRIGMSAAFRGAA
                     AGLGSELYRGAHAYYSEINARGGLFGRSITVVALDDGYNPDPCIRNTIQLLDRDKVFL
                     LSNYVGTPTLTRALPVIKQYADQNVVLVGNFTGAQPQREPPYVDQVFNVRASYRQEMA
                     ALVERFWQTGARRFGVYYQIDAYGRSGTDGVARALAARNAKIVAEATYVRNAKFEEDM
                     TIAVKALREGGSEVVLCTGAYQGCAAFVRSARDAGWTAPISNVSFVGSDAMLGLLVKH
                     GRAKGRDYTRALINSQVVPSYDDTGLPGVVEDRTLMDKHNPQVPEALRDAAYAPQKYS
                     FISLEGYVNARVIVEALRRAGATAGRSSFRQALESLRGLDLGIGAPLSFTAERHQGLD
                     SVYFTRVEGDRWVPVADWSAAVKA"
     gene            9490..11313
                     /locus_tag="VFX87_01140"
     CDS             9490..11313
                     /locus_tag="VFX87_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4993535.1"
                     /translation="MRKRAVDPGPLVETASVPVSRARFGLLGKITIFLVLVLLPVAIV
                     SWVIAYRSLQENLSREFTDKGSAIANSLSSSAADLVVNRDASTVQAQVDEFAKISGVA
                     YVMVYDPQRTLIAHTFAPVVPKGIIEKNVVSGQATRPQVQELTYLDPRGGAERKIIDI
                     GVPMLGGRLGTVRVGMDRSIIDTAAVGAGNFLLLILGGIAAAAVLAGVIFAQRITRPV
                     NALMSVAERVGRGDLSELVPVTSRDEIGQMAVTFNESIVRLRSLVQTETDRDEERRKR
                     EELQQNIIKFLDTVVEIGKGDLTRRGEVSWDVLGNVVDAINLMIEEISSIVGDVRQAA
                     LRVASGSQDTIRITGELAEAAQAQTRDAVSVTRSVADVTVSVRQVADSADQSAKAARQ
                     VLQAASKGEGAVRNSLASMQQIRAEVQTISKKIKSLGDRSLEISNIVNTIQDIAAQTH
                     LLALNASIEAAGAGEAGLRFSVVADEVRKLAERATQATRDIGTLIKGVQVETQEAVVA
                     MESGTREVESGYEVSLKAEEALQEIGKISQTSAELAAEISHASQTQVRGAEAAAGAVQ
                     AIAGAAVKTEQGVQQSRKNVEQLARLAEELTAKLSRFKLAS"
     gene            11318..14254
                     /locus_tag="VFX87_01145"
     CDS             11318..14254
                     /locus_tag="VFX87_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993536.1"
                     /translation="MADRRERELLLGIFLMEAWDSAGAIEEGLGRLTGEEAPTEEALA
                     PLAVFAHRLKGSAALHGFPGVSDLAAGTERLLELAPAASAEERARSAVLLEGLVGLLK
                     EVFDGISAVGMEDSARIGEFKARYPAFFSSATPAARLEPGPAAVAPAPTPVSPLIADL
                     RRFFAEGGEVLEYFLPEAAEHLEAMTTAMLAVEGGARDEDTLATVFRAAHTLKGAAYT
                     VGCAPLGDATHQIEDLLVAVRERRLPVTPAVTEAIFAGTAAIKRVLQSGAAVSDEAVQ
                     ALERATSDLRGLTASESLRERAAPRPAVEVPELVASEAPDLALSTSAELPAFVRPEFL
                     ARLEALPRPAPRVASALTGPSIRVPVERLDTLMNLVGELMIARSRLDRRLAQLDQVSE
                     LLLASRTRLGQVGRDFEFRRQAPTLPREGRDDGGERRDISELFAELEFDRYDDISILA
                     RSVTEMSADVAEVQSQHAILLRDIREDTVHIQRLTAALRKEVTRARMVPIGRLFARVA
                     PQVREAARATGKAVTLTVRGESAEVDTGIIEQIADPLLHLIQNAVAHGIESATERESR
                     GKAASGHVTLSASQEGSFILVEVEDDGRGIDTEMLRRHAAERGFLPAAEAATLPEGEI
                     LNLIFVPGFSTASAVTQTAGRGVGLDVVRTNVSRLNGEIHVETEAGRMTRFVLKLPLT
                     MVIADALIVRCGAETVAFPLTAVSAMRSVAPHEVLTAGGRETVQLDDRVIDLVRLERA
                     LGLVASPPPSRLPVVVMRGGGKPFGVVVDELLGKEEIVIKRLGALVDGVGPFSGATIS
                     GEGRVILLVDPTLLREAAARKPAAPVREPAGASLDAPDRRLTVMLVDDSVSIRKFVGQ
                     MLEKAGFRVVAAIDGQDALEQLLDRTVDVVVTDLEMPRVNGYALIEDLRRRSATREVP
                     VIVMTSRAGEKHAGLARRLGVRHYITKPVDEQTFVRIVEAVAAGERALEGSALAGSTR
                     AGSLVE"
     gene            14258..>14608
                     /locus_tag="VFX87_01150"
     CDS             14258..>14608
                     /locus_tag="VFX87_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993537.1"
                     /translation="MADAKVLVVDANQDSRVALAHALAEAGYEAAVATSGSFALTMLE
                     WEQPDLIVSYAKVQDMDGYELFTMVRRDPATKDTPFLLLAGRDRPIALAATEAGVDMT
                     VTGDFTLATVMSRIK"
BASE COUNT         2177 a         5022 c         5287 g         2122 t
ORIGIN      
        1 gtagaagttc acggtggcgc cgatcgccgg gttcagcgtg gccgcgaaca gcgcgagctg
       61 tccgcccatg cagaagccga ccgctcccag cttgggggtc gacgagtgct gcgcgagata
      121 cgcggtggcg cccttcaggt ccttctccgc gaagagcccg agcaccggac cgttcagctt
      181 gcggtagtcc ggcttgacgt tcggatggat gccgtagaag tccaccaccg cgcccacgtt
      241 cacgttggtg catccggcga agagggcgag ctgcccgccc atgcagaagc cgaccgcgcc
      301 cagcttggcg gtcgaggagt ggccggccag gaacttggcg gcgcccgaga gatccttctc
      361 ggcgcgctgg atgttgagcg ccatgaagag cttgcccgcc gcgtccggct cggtcgcggt
      421 ctggccgtgg tagagatcgg gggcgagcgc cgagaagccg gcggccgcga agcgatcgca
      481 cacgttcttg atgtggccca ccagccccca ccactcctgg atcacgagga cgcccgggcc
      541 cttgccggag gcgggagtgg ccagatagcc cttcgcgctg tgcccgttgc tcggaaagtc
      601 gaccatcttg ccggccatct cggtttctcc ttcttagggt tgaatgtgga tgacttcggt
      661 tgggcggttg gggtcttctc aggttgcttg aacgtgaaag accttggttt ggcggtttgg
      721 cttcttcgcg caagcgctca tccgacctgc cttgccttgt ggtccggatc gccttcgacg
      781 cggggctttg cgcgccggcg gcgtctagtc tagcgattct ggtgcttcgc gcaagcgcta
      841 ggactcgggc ttgtcggggg cgggtttctt cgcggggccg ggggtgacgc ggacggtgag
      901 gacgcggcgc ttgtcggctt cgagcaccgt gaaggagtaa cgcccgaccc ggaagacctc
      961 gccgaccagg gggatgcggt ggagcgtggc gagcacgagg ccggccaccg tctcgaaatc
     1021 gcccttggga aggtcccagt ccagcgcctc gttgacctcg tcgaggcctg cccgccccgc
     1081 cacccggtag ctgccgtcgg gcaggcgctc caccgtgtcg ggggtgcggt cgtgctcgtc
     1141 gcggatctca cccacgatct cctcgacgat gtcctccacc gtgacgatgc cgacggcgcc
     1201 gccgtactcg tccaccacca ccgcgagctg cacgcggttc ttctgcatct cgcgcagcaa
     1261 ctcgtcgatg cgcttggtct ccgggacgta ggtggcgggg cgcatcagcg agcgcacgtc
     1321 cggcccctga acgccccggc tgaagagatc catcgtggtg accacgccca cgatattgaa
     1381 cgcgcggtcg gtgaagacgg gaatgcgaga gaagccccgc tcctggatga ggcggatcgc
     1441 gtccgcgggc gaggcggtct ccgggagcgc ggccacgtca atcagcggga ccatgacctc
     1501 gcgcaccgcg gtctccccga ggtcgaagat cttgtcgatc atctccgcct cggtgacggt
     1561 gacgtcggcc tcctccggct ccatctgcag cagcagcttc agctcctcgc gcgagacgaa
     1621 ctggcgggtt ccggtgggcc cgcgtcccac caaggccagc gcgcgcccca ccagggcgtt
     1681 ggcgccccag gtgagcggcg agaggacctt gcccagcaac tccaccaccg ggaacaggat
     1741 caggatgagg ccggtcgccc gctcccgcgc caccgccttc gggatcacct cgccgaagac
     1801 gagcatgagc ggggtgaggg cggcggtggc caggagcgcc gccccgccgc ccaccaccgg
     1861 gatgagcgcc caggtcgcca cgctggaggc ggtgatgtgg gcgatggtga cccccatcat
     1921 ggcggtggac aggaggcgct ccgggctccg gaacgcctcg aggtaacgaa tggccgtccg
     1981 gtggccgccc tccgccatgt ggcgcagccg gagccggttg gccgcgatga acgacatctc
     2041 ggccgccgag aagagcgcgg tcaccagcag ggagagcccg atgagccaga cgtagatcac
     2101 gtggcctccg gcggcccggc gacctcgggc agggcgacca gcacctcgag cacgcgcgtc
     2161 cgctcgacct tctccaccga cacgcggacc ccgtcctcgg tctccttctt ctcgccccgg
     2221 tggggcacac ggccgaagag atcgagcacc cagcccccca ccgtgtcgaa ggactcgtcg
     2281 ggaatcgcga gcccggtcgt ggcgttcagc tcgtggatgg ggaggcgccc ggagacccgg
     2341 aaggtgcgcg ggccgacgcc ctggatcagg cgctcgtcct cgtcgtactc gtcgcggatc
     2401 tcgccgacca gctcctcgag caggtcctcg agggtgacga ggcctgcggt gccgccgtac
     2461 tcgtccacca cgatggccag gtggagcttc ttggcctgga actcgcgcag cagcgcgtcg
     2521 gcccgcttcg actccggcac gaagtaggga gggtggagat gcgcgcggag ctcgaacgcg
     2581 ggcggcagcc cgccgaggtg cggcagcagg tccttggtgt agagcacccc gacgatctgg
     2641 tcgatggttt cctcgaacac cggcacacgg gagtggagct gctcgcgcaa cagcggcagg
     2701 atctcccccg acggcgtgga catgtcgagg cagaacatgt cgggacgcgg caccatcacc
     2761 tcgcgcacca gggtgtcctc cagttcgaac accttgtgga tcatctcgcg ctcggtgcgc
     2821 tccaccacgc cttcgcgcgc gcccacgtcc accagcgtac ggagctcttc ctcggagatc
     2881 tcgggctgcc cgaccttccg ctcggacccg accagccgaa gggtgaccgc ggtgaacgcg
     2941 ccgagtccga tccggatcgg cttcagcagg acggagagcc acgccaccgg ccggttcacc
     3001 cagctcgcga accgcagcgg gtgctcgact gccagggtca tgggcagcac ctcgccgaac
     3061 acggtgagca ggaacaccat ggcgccgatg gcgatgggga ggcccttgcc gccgaagatc
     3121 cgctcggcga tggccgcggc gagcgccgag gccccgatgt tcaccaccgt gatgccgacg
     3181 aggagcgtga ccagcagctc gttgggctgc gcgagcagcg gcgcgagcgg gccgggcgtc
     3241 ttgccctgct gttcggccag ctcctttagc cgcgtccggc ccagggagaa gtacgcggcc
     3301 tccgcgccgg agagcagcgc ggagaccagg agcaggatcc cgaggacaat cagctcggga
     3361 aacacccggc tatccatgga agtgctcgaa gccgccgcgc atcgtgaccg gcgcgccgtc
     3421 ggcgtccacg aagacgatct cgcccgtggt cccctcgatc cgtccgatca cggtgagcgc
     3481 ggtgccggtg gcctcggcga ggcccgcgat gaggcggtcg gccgcggcgg ggtcgcacgt
     3541 gagcaggagc tcgtagtcct cgccgccgct caccgcccac tcccacgcgt cacccccgag
     3601 cgcgcgcgcc gcctcccgcg ccgccgccgc gaccggcagg cgctcgagtc gcacgcgggc
     3661 gcccaccccg ctctcccggc agatgtgccc gaggtccgtg gcgaggccgt cggagcaatc
     3721 catcatggcg cggacgccgg gcgcctgacc cagccaccgc ccctccgcga cgcgtgcgcg
     3781 gggccggagg tgggcccgcg tgatctcggc gagcgccccg ccggccaggc gcgcgtcgcg
     3841 ggcgcggtcg agcccgatct cgagcgcgtg aaggccggcg gcggaggctc ccaggctgcc
     3901 ggtgagcgcc accgcgtcgc cggcccgggc gtgcgagcga agccgcggct cgcccggatg
     3961 ggtcccgagc agcgtgacgt tcaccatcca gccgcccggg gcggccgacg tgtcgccgcc
     4021 gacgacggtc acgccgtgag gcgcggcggc ctccgccatg cccgcgtaga aggtgtccac
     4081 cgcctccacg tcggtatccg cggggacggc cagcgccacc agcgcccagc gcgggatgcc
     4141 ccccatggcc gcgatgtcgg agaggttgac ggccatcgcc ttccagccga tatcggcggg
     4201 tgcagcggag atccgccgga agtggacgtc ctcgatcagc agatcggtgg tggcgagcag
     4261 cttgtggccg ggcggcaccg tgaggaccgc ggcgtcgtcg ccgatgccga cctccactcc
     4321 ggccgcggct tcgctcgcgg cggcccggct ccggatgcgg cggatcagcc cgagctcgcc
     4381 cagcccgccc aggctcaccc gttcgggtgg ttccgcgttc atgcgggcgc ggtgaccggc
     4441 gtcagcgcga ggaagttggt cagcagcgcc ttgccctggc tcgtgaggat ggactcggga
     4501 tggaactgca cgccctcgac cgggtagtgg cgatgccgca gccccatgat ctccccgtcg
     4561 tccgcccggg cggtgatctc gaggtcggtg ggcaggcccg cctcgagcac gaccagcgag
     4621 tggtagcgcg tcgcctcgaa cctcggcggc agcccgcgga agacgccgcg cccatcgtgc
     4681 gtgatcgggg acgtcttgcc gtgcatctgc cgtcgcgcgc gcgccaccgt gcccccgaag
     4741 gcctggccga tcgcctggtg accgaggcaa accccgagga tcggcacgcg cgcgtaaaag
     4801 cgccggatca actcgaggga gatgccggcg ccgtccggac tgccggggcc cggggagatg
     4861 acgatgccct cgggcaccag cgcctccacg tcgtccgagg tgagtgcgtc gttgcgggcc
     4921 acccggatgc gggccccgag ctcgcccagg tactggacga ggttgtaggt gaacgagtcg
     4981 tagttgtcga gcaccaggat cacgccggct gctcctgggc cgcgatgcgg agcgccagga
     5041 tcatgccccg ggccttcgag gcggtctcga gccactcggt cttgggatcg gagtcggcca
     5101 cgatgccggc gcccacctgg atggaggcgc ggcgcccgtg gcacaccacg gtgcggatcg
     5161 tgatgcagga gtcgaggttg ccggagtagg agatgtagcc gactgcgccc ccgtagggac
     5221 cccggcgggt cggctcgagc tcctcgatga tctccatggc gcggatcttg ggcgctccgg
     5281 tgagggtgcc ggcggggaag caagcggcga gcacgtcgaa ggcgtccttg cccgactcga
     5341 gccggccccg cacgtgactc acgaggtgca tgacgtgcga gtagcgctcg accaccatga
     5401 actccgacac ctcgacgctg ccgagtcggc tcacccggcc cacgtcgttg cgcccgaggt
     5461 ccaccagcat cacgtgctcg gcccgctcct tcggatccgc ccgcatctcg tccgccagcc
     5521 gggcgtcctc ctgctcggtc cgaccccggg gatgtgtgcc cgcgatcggc cgctcttcga
     5581 cccgctcgtc ctcgagccgc accagcacct ccggggacga ccccacgatg ctggtcttgc
     5641 ccaggcgcag gaagaagaga tagggcgacg gattgacggt gcggagcgcg cgatagaccg
     5701 tgaacgggtc ggcccgcagc tcggtgtcga ggcgccgcga cagcaccacc tggtaggcgt
     5761 cgccggcggc gatgaactcc ttcgcccggc gcacgccgtc cgtgaaggcg gcctcgtcca
     5821 tcgtcgaggt gaatccctcg tcccccaggg ccatgagcgg ctccaccggc ggcagggtga
     5881 gcggggcggg ggagcgcgcc ggccgccgga ggttacccag cagcatgccg atcttgaccg
     5941 cggcggcgtc gtacgcgcgg tcgagcgagg ccggatcggt cttggtgatg tgggcattcg
     6001 cgatcaccag cagccggtgc cgcaggttgt cgaagaccag caggctatcg gtgaacatga
     6061 ataccgcgtc gggcagcttc tgatcgtcgg tggtcagggt ggggagccgc tcgacgtgcc
     6121 ggatcatgtc gtaggacaag aagcccaccg ccccgccctg gaatcgcggc agcccgggca
     6181 ccggaaccgg cttgaactgg gcgagcaggt cccggagcgc cttgaacgga tccgagccgg
     6241 ccagcagctc gatggtgccg tcggcccgac gcacgctgat gcgcttgccc ttggcggtga
     6301 agaccatcag ggggtctgtg ccgaggaagg agtagcgggc ccacttctct ccgccctcga
     6361 ccgactccag caggaaggcg taggggccgg ggcggagccg gaggaaggcg gacagcggag
     6421 tgtcgaggtc ggcggccagc tcggcgaaga ccgggatcag attgccctgt ccggccaggg
     6481 cacggaactc ctcgcgcgtg ggagagagcg gggggagacc cggccccacc gttgacatgg
     6541 atgagtcgct caggcgtttg cgtccagctt cttcttgagc tgtgccttcg gaaccgcgcc
     6601 gatcacctgg tccatcacct tgccgccctt gacgaacagg atggtgggga tggagcggat
     6661 cccgtagcgg gccgcaagcg ccggctgctc atccacgttc accttcgcga gggtcaccgc
     6721 gccgttgcct tcgcgcacca gctcttcgag cgtgggagcg atggcgcggc agggggcaca
     6781 ccattcggcc cagaagtcca ccatcagcag ctcagtgtgc ttgtccagtt ccgcgtcgaa
     6841 gctggcctcg gtgaggtgga gcgtgctatc ggccattgat ccagggtctc cttatggtcg
     6901 ggcgggcttg gtgcgggaca gcgggtgaga gttccataaa aaacgcacag tcacgctagc
     6961 atacgccccc aaccatcgca agttggcccc cggccccggg cgggtcgctg caggcacatg
     7021 ccccatgggc tgggcaggtg ggccgacggt tcggggcact ccttctgaca gtaggcgtca
     7081 cctgcccgac gtccgcccgg tgtcaccttg ccgcggtagg ccccaaaacg accctggagg
     7141 gccggaaagc cttgaatcca cggcattgac ctggacacgc cgagaacagc atccaagacg
     7201 gcggaaaacc tcggttggta gggggtttgc agtagatgaa ccccccgaaa agccataccg
     7261 ggtcggattc gttccgttag ccacgtgcac tcgcagccga gggagggtcg ggtcgtgggc
     7321 aaggtgatgg ttgtcgatga cgccgtgtca gagctgaagc tgatcgaggg cattctcaag
     7381 gcagccggtc atcaggtggt gtcgtacgac cacggcgagc tcctggaaga tcgggtcgtc
     7441 gaggagcggc cggacctgct gctgctggac atcgtgatgc ccaagcgcaa cggctacgag
     7501 gtgctgcgag ggctccgacg cgatccccgc accaaggaca ccccagtggt gttcgtgagc
     7561 tccaagaacc aggagagtga tcgcctgtgg gggcagcggc agggcgcgga ggactacctg
     7621 gccaagccgt tcaccgcgga ccagctcctc ggcgtggtgc gccagttcgt gaaatagcgc
     7681 tgatgaccca gcctcccgcg atcccggtgc tgccggctgc tccggatgcc cctccggcgc
     7741 cgcgcgcctg cctgttcgct ctggccggcg cgcggttcgc agtggacgtg cggagcgcgc
     7801 gcgaggtggt gctcttcgag gagatcaccg tggttccccg ggccccccgg cacctggtgg
     7861 gcgtggcgaa cctccggggc accgtgatgc cgatcgtcga cgtccgggag ctgctgggcc
     7921 tgccggcatc gcgccccggg cgcagtctcc gcacgctggt cgtgcgcgat ggggccgtgc
     7981 aggcggcgct ggtggtggac accgtgctcg ggctcgagcc cttcgaagcc gtgttgccgg
     8041 ccgacaactc gaccgctgcg gggatgggcg ggccccgccg gctgatgacc gggtggatcc
     8101 agtgggccgg ggagaccctg ccgctgctcg acgtttcgaa gatcctggcc gcccttcgcc
     8161 ccccgacccc gacggtcgcg caggaggcat cggcatgaac cgcagagagt tcctcggcgg
     8221 cgtcctctcg accggtctcg cgagcaccct cctcccgggc ggcggcgcgc gggcccaggc
     8281 tggcgagatc cggatcggca tgagtgccgc gttccggggc gccgcggccg gcctcggcag
     8341 cgagctctac cggggcgctc acgcctatta cagcgagatc aacgcgcggg gcggtctctt
     8401 cgggcgctcc atcacggtgg tcgcgctcga cgacggctac aacccggacc cgtgcatccg
     8461 taacacgatc cagctcctgg accgggacaa ggtattcctg ctttccaact acgtaggcac
     8521 cccgacgctc acgcgcgcgc tacccgtcat caagcagtac gcggaccaga acgtggtgct
     8581 ggtgggcaac ttcaccggcg cccagcccca gcgtgagccc ccctacgtcg atcaggtctt
     8641 caacgtccgg gcctcgtacc ggcaggagat ggcggcgctg gtggagcgct tctggcagac
     8701 gggcgcgcgg cgcttcggcg tctactacca gatcgacgcc tacggtcgca gtggcaccga
     8761 cggggtggcg cgggcgctcg ccgcgcgcaa cgcgaagatc gtggcggagg ccacctacgt
     8821 gcgcaacgcc aagttcgagg aggacatgac catcgcggtc aaggccctcc gtgagggcgg
     8881 cagcgaggtc gtgctctgca ccggcgccta ccagggctgc gccgccttcg tgcgctcggc
     8941 gcgcgacgcg ggctggaccg cgcccatctc caacgtctcg ttcgtggggt cggacgccat
     9001 gctcggattg ctggtcaagc acggccgcgc gaagggtcgt gactacaccc gcgccctgat
     9061 caattcccag gtggtgccga gctacgacga cacgggtctg ccgggcgtgg tcgaggaccg
     9121 caccctcatg gacaagcaca acccgcaggt gcccgaggcg ctccgcgacg ccgcctatgc
     9181 gccgcagaag tacagcttca tcagcctcga gggctacgtg aacgcgcggg tgatcgtcga
     9241 ggcgttgcgc cgggcggggg ccaccgcggg gcggtcgtcc ttccgccagg cgctggaatc
     9301 gctccgcggg ctcgatctcg ggatcggcgc gccgctcagc ttcacggccg agcggcacca
     9361 ggggctggac agcgtctact tcacacgggt cgaaggcgat cgctgggtgc cggtcgcgga
     9421 ctggtccgcg gcggtcaagg cctgagcatg ggcatggacg gtctggagcg gggctggagg
     9481 aggcacgcga tgaggaagcg cgcggtggac ccgggacctc tcgtcgagac cgcgtccgtc
     9541 ccggtctccc gggcgcggtt cggcctgctc ggcaagatca ccatcttcct cgtgctggtc
     9601 ctgctgccgg tggcgatcgt gagctgggtc atcgcctacc ggtcgctgca ggagaatctg
     9661 agccgtgagt tcaccgacaa gggcagtgcc atcgccaaca gcctgtcgag ctccgcggcg
     9721 gacctggtgg tgaaccgcga cgcgtccacc gtgcaggcgc aggtcgacga gttcgccaag
     9781 atctccggcg tggcctacgt catggtctac gatccccaac gcaccctcat cgcccacacc
     9841 ttcgccccgg tggtgcccaa gggcatcatc gagaagaacg tggtgtcggg gcaggccacc
     9901 cggccgcagg tccaggagct cacctatctc gatccccgcg gcggggccga gcgcaagatc
     9961 atcgacatcg gcgtgcccat gctgggcggc cggctcggga ccgtgcgggt gggcatggac
    10021 aggtcgatca tcgacaccgc ggcggtcggg gcgggcaatt tcctgctcct gatcctggga
    10081 ggaatcgcgg cggccgcggt gctcgcgggc gtgatcttcg cgcagcggat cacgcggccg
    10141 gtcaacgcgc tgatgagcgt ggccgagcgc gtgggccggg gcgacctctc cgagctggtg
    10201 ccggtcacct cgcgcgacga gatcggccag atggcggtca ccttcaacga atccatcgtg
    10261 cggcttcgct cgctggtcca gaccgagacc gaccgcgacg aggagcgccg caagcgcgag
    10321 gagctgcagc agaacatcat caagttccta gacacggtgg tggagatcgg gaagggcgac
    10381 ctcacccggc gcggcgaggt gagctgggac gtgctcggca acgtggtgga cgccatcaac
    10441 ctgatgatcg aggagatctc ctcgatcgtc ggcgacgtgc ggcaggccgc cctccgcgtg
    10501 gcctcgggct cgcaggacac catccggatc accggcgagc tcgcggaggc ggcgcaagcc
    10561 cagacccgcg acgcggtgag cgtgacccgc tcggtggcgg acgtgacggt gtcggtgcgc
    10621 caggtcgcgg acagcgccga ccagagcgcc aaggcggcgc gccaggtgct gcaggccgcg
    10681 agcaagggcg agggagcggt gcgcaacagc ctggcgagca tgcagcagat ccgggccgag
    10741 gtgcagacca tctccaagaa gatcaagtcc ctcggcgacc gctcgctcga gatctccaac
    10801 atcgtgaaca ccatccagga catcgcggcc cagacgcacc tgctcgccct gaacgcctcc
    10861 atcgaggcgg cgggtgcggg cgaggcgggg ctgcggttct cggtggtggc cgacgaggtc
    10921 cggaagctgg cagagcgcgc cacccaggcc acccgggaca tcggcacgct catcaagggc
    10981 gtgcaggtcg agacccagga ggcggtggtg gcgatggaat ccgggacccg ggaggtggag
    11041 tccgggtacg aggtcagcct caaggcggag gaggccctcc aggagatcgg gaagatctcg
    11101 cagacctccg ccgagctcgc cgccgagatc tcgcacgcgt cgcagacgca ggtccgaggc
    11161 gccgaggcgg ccgcgggcgc cgtgcaggcg atcgcgggcg cggcggtcaa gaccgagcag
    11221 ggcgtgcagc agagccggaa gaacgtcgag cagctggccc gcctggcgga agagctgacc
    11281 gccaagctgt cccggttcaa gctggcgagc tgacgacatg gccgatcggc gcgagcgcga
    11341 gctgctgctg ggcatcttcc tgatggaggc ctgggatagc gccggggcca tcgaggaagg
    11401 gctcggccgg ctcaccggcg aggaggcccc gaccgaggag gccctcgcgc ccctcgccgt
    11461 gttcgcgcat cgcctgaagg gctcggccgc gctccacggg ttccccggcg tctcggacct
    11521 ggccgccggc accgagcggt tgctcgagct ggcccccgcc gcgtcggccg aggagcgggc
    11581 ccggtcggcc gtcctcctgg aaggcctcgt cgggctcctg aaggaagtct tcgacggcat
    11641 cagcgcggtc gggatggagg actcggcccg catcggggag tttaaggccc gctatccggc
    11701 gttcttctcc tccgcgaccc ccgcggcccg gctagaaccg gggccggcgg cggtcgcgcc
    11761 ggctccgacc ccggtctcgc ccctgatcgc cgacctgcgg cggttcttcg ccgagggtgg
    11821 cgaggtcctc gagtacttcc tgcccgaggc cgccgagcat ctggaggcca tgaccacggc
    11881 catgctggcc gtcgagggtg gcgctcggga cgaggacacc ctcgcgaccg tcttccgggc
    11941 cgcgcacacg ctcaagggcg cggcgtacac ggtcggctgc gcgccccttg gcgacgccac
    12001 ccaccagatc gaggatctcc tggtcgcggt gcgcgagcgc cggctccccg ttacgccggc
    12061 ggtgaccgag gcgatcttcg cgggaaccgc ggccatcaag cgggtgctcc agtccggggc
    12121 tgcggtctcc gacgaggccg tgcaggcgct ggagcgcgcg acgagcgacc tgcgcggcct
    12181 gacggcctcg gagagcctgc gcgagcgcgc ggccccccgg ccggcggtcg aggtccccga
    12241 gctcgtcgcg tcggaggccc cggatctcgc gctgtccacc tcggcggagc tgcccgcttt
    12301 cgtccggccg gagttcctgg cgcggctcga ggcgctaccg cgaccggccc cccgcgtcgc
    12361 gagcgcgctg accggtccca gcatccgggt gcccgtcgag cggctcgaca cgctgatgaa
    12421 cctcgtgggc gagctgatga tcgcgcggag ccgcctggat cgacgcctgg cccagctcga
    12481 ccaggtgagc gagctgctcc tcgccagccg cacgcgcctg ggccaggttg gccgtgactt
    12541 cgagttccgc cggcaggcgc ccacgctgcc ccgcgagggc cgcgacgacg ggggggagcg
    12601 gcgcgacatt tcggagctct tcgccgagct cgagttcgac cgctatgacg acatcagcat
    12661 cctggcccgc agcgtgaccg agatgtcggc cgatgtggcc gaggtgcaga gccagcacgc
    12721 gatcctgctg cgcgacatcc gggaggatac cgtccacatc cagcgcctga ccgcggccct
    12781 ccgcaaggag gtcacgcgcg cgcgcatggt gcccatcggc cggctcttcg cgcgggtggc
    12841 cccgcaggtg cgggaggcgg cgcgggccac cgggaaggcg gtgaccctca cggtgcgcgg
    12901 ggagtcggcg gaggtggaca ccgggataat cgagcaaatc gccgacccgc tgcttcacct
    12961 gatccagaac gcggtggctc acggcatcga gtcggcaacg gagcgcgaga gtcgcggcaa
    13021 ggcggcctcc ggtcacgtga ccctgtccgc ctcccaggag ggtagcttca tcctggtgga
    13081 ggtggaggac gacgggcgcg ggatcgacac cgagatgctg cgccgccacg cggcggagcg
    13141 cggattcctg cccgcggccg aggcggccac gctgcccgag ggcgagatcc tcaacctcat
    13201 cttcgtgccg ggcttcagca ccgcctccgc ggtgacccag accgctggtc gcggggtcgg
    13261 cctcgacgtg gtgcggacga atgtcagccg gctcaacggc gagattcacg tcgagaccga
    13321 ggccgggcgc atgacgcgct tcgtgctgaa gctgcccctc acgatggtga tcgcggacgc
    13381 gctgatcgtc cggtgcgggg cggagaccgt ggcgttcccg ctcaccgcgg tgagcgcgat
    13441 gcgctcggtc gcgccccacg aggtcctgac cgcgggcggg cgggagacgg tgcagctcga
    13501 cgaccgggtc atcgacctcg ttcgcctcga gcgggcgctg ggcctcgtcg cgtctcctcc
    13561 gccctcgcgg ctgccggtgg tggtgatgcg gggcggcggc aagcccttcg gcgtcgtcgt
    13621 ggacgagctc ctgggcaagg aagagatcgt gatcaagagg ctgggcgcgc tcgtcgacgg
    13681 cgtgggcccg ttctcgggcg ccacgatctc cggcgaaggc cgcgtgatcc tgctcgtcga
    13741 tccgaccctc ctgcgcgagg cggcggcgcg gaagccggcc gccccggtgc gcgagcccgc
    13801 gggcgcgtcg ctcgacgccc cggaccgccg tctcaccgtc atgctggtgg acgattcggt
    13861 gagcatccgg aagttcgtgg gccagatgct ggagaaggcc ggcttccggg tcgtcgccgc
    13921 catcgacggg caggatgcac tcgagcagct cctggaccgg accgtggacg tggtcgtcac
    13981 cgacctcgag atgccgcggg tcaacggcta cgccttgatc gaggacctgc ggcggcgttc
    14041 ggcgacccgc gaggtgccgg tgatcgtgat gacgtcgcgc gccggcgaga agcacgccgg
    14101 cctggcccgg cgcctgggcg tgcgccacta catcacgaag ccggtggacg agcagacctt
    14161 cgtgcggatc gtcgaagcgg tcgcggcggg ggagcgcgcg ctcgagggct cggcgctggc
    14221 cggctccaca cgcgccggga gcctggtgga gtaggcgatg gccgacgcca aggtgctcgt
    14281 ggtggacgcc aaccaggaca gccgggtcgc gctggcccat gctctcgcgg aagccggcta
    14341 cgaggcggcg gtcgcgacga gcgggtcgtt cgcgctgacc atgctcgagt gggagcagcc
    14401 cgacctcatc gtgagctacg ccaaggtgca ggacatggac ggctacgagt tgttcacgat
    14461 ggtgcggcgt gatcccgcga ccaaggacac gcccttcctg ctcttggccg gccgcgaccg
    14521 gcccatcgcg ctggccgcca ccgaggccgg tgtggacatg acggtcaccg gggacttcac
    14581 cctggccacc gtgatgtcgc gcatcaag
//
LOCUS       DASUFA010000033         4938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_694844, whole genome shotgun sequence.
ACCESSION   DASUFA010000033 DASUFA010000000
VERSION     DASUFA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694844"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..824
                     /locus_tag="VFX87_01155"
     CDS             108..824
                     /locus_tag="VFX87_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4993538.1"
                     /translation="MTSGARAAVVFLVDVDNTLLDNDLVIDDLRRHLDLAVGPEGRAR
                     YWALFEQLRVELGYADYLGALQRYRLEFPHDLRLLTVSGFLVDYPFANRLYPGSLDVL
                     ARFREWGPTVILSDGDVVFQPRKVERSGLGRAVDGVLIYVHKERELDDVERRYPAEHY
                     VLVDDKLRVLAAVKRAWGARVTTVFPRQGHYAHDAEALAGYPPADLTVERIGDLMGHD
                     LSTLRARAADGAAAGPATGR"
     gene            829..3198
                     /locus_tag="VFX87_01160"
     CDS             829..3198
                     /locus_tag="VFX87_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX4993539.1"
                     /translation="MAMSAEQIRLLDAYWRAANYLTVGQIYLRDNPLLREPLTREHVK
                     PRLLGHWGTSPGLSFIYAHLNRLVRETDADVIYLAGPGHGGPAIVANVYLEGTYSEIY
                     PDVSRDAAGLRRLFRQFSTPGGIPSHVSVPTPGSIHEGGELGYVLVHAFGAAFDNPEL
                     IVAAVVGDGEAETGPLAGSWAGVDFLNPARDGAVLPILHLNGYKIGGPTLLGRMGDDD
                     IVSLLGGQGWEPHVVAGDDPGVMHARFAQVLDDCHARIRAVRAEARRPGVGAARATRP
                     RWPAIVLRTPKGWTGPKVVDGLPVEGTFRAHQVPVDRVREDPAHLALLEAWMRSYRPE
                     ELFDERGGLRAELAALAPTGDRRLGANPHANGGRVLQPLDLPDFADYALPVPQPAAEL
                     HGSTRQLGKFMRDIFTRNRAAANFRLFCPDETNSNRLGDVFEVENRCSVLPRTAADDH
                     VAPDGRVMEILSEHCCQGWLEGYLLTGRHGLFASYEAFAMVSASMTVQHAKWLQEADR
                     LPWRAPVASLNILLTSTAWRQDHNGFSHQGPGLIDVVLSKKGTVSRIYLPPDANSLLS
                     VADHCFRSRNYVNLIVIDKQPQLQWLDLEAARAHCARGASVWPWAGSPDDGPPDVVLA
                     AAGDVPTLEVIAAAWLLRRRAPSLRVRVVNVVDLMTLFAPRFHPHGLTDEAFVALFTA
                     ATPVVFAFHGYQRAVHELVHGRPNAERFHVRGFNEEGTTTTPFDMVVRNGMSRYHLCL
                     EALRRAPQGEARGVALVAHCRAMLDRHPGYIREHFEDMPEVRDWRWSEP"
     gene            3198..4379
                     /locus_tag="VFX87_01165"
     CDS             3198..4379
                     /locus_tag="VFX87_01165"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX4993540.1"
                     /translation="MPGAPIILCLNAGSSSLKFSVWDGDECLGEGEVEEIGRPGAFAW
                     LGKPDGVPRRLPGRWADHGEAAAGVFALLGEHTLPAPAGVGHRLVHGGQWHLAPERVT
                     PTLLDDLRALLPLAPLHLRSGLAGVEAVAARFPALPPAVCVDTAFHRDLPEVARRMAL
                     PHELSDAAGIRRFGFHGLSYEYVMERLGPAGRGRVVIAHLGNGASMAAVRAGRPVDTT
                     MGLTPAGGFMMGTRTGDLDPGVLLYLMREQGYDASRLDRLVNKESGLLGVSGASGDMK
                     TLLERRERDGAAALAVAMFCYQARKQIGAFAAAMGGLDTLVFTGGIGERAAPVRAEIC
                     DGLGHLGVRLDPARNAAHADPLSAPGDSCAVRVVLTREDLMIARHSRAVLFAERREEI
                     R"
     gene            4376..>4938
                     /gene="pgi"
                     /locus_tag="VFX87_01170"
     CDS             4376..>4938
                     /gene="pgi"
                     /locus_tag="VFX87_01170"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119262.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX4993541.1"
                     /translation="MISQSEAWAALRKHHGQIRDVHLRTLFAEDPTRGERLALEAASL
                     YLDYSKNRVTDETLRLLLALAEAAGLRARIDAMFSGEKINVTEQRAVLHVALRAPRGT
                     RILVDGQDVVPGVHAVLDRMADFSTRVRTGAWTGFTGKAIRNVVNIGIGGSDLGPAMA
                     YEALRHYSDRRLGLRFVSNVDGTDFMEA"
BASE COUNT          653 a         1775 c         1814 g          696 t
ORIGIN      
        1 ttcacctcgc gcggcaacgg cgagttcgcg gccaaggtgc tctccgcact gcgattcgag
       61 ttcggcgggc accaggagaa gcccgccgag gacgggcccc ggcgcgcgtg acgtccggcg
      121 cgcgcgccgc ggtcgtgttc ctggtggacg tggacaacac gctcctcgat aacgacctcg
      181 tgatcgacga cctgcggcgg catctcgacc tggcggtggg gccggagggg cgggcgcgct
      241 actgggccct cttcgagcag ctccgggtgg agctgggcta cgcggactat ctcggggccc
      301 tgcagcgcta ccggctcgag ttcccgcacg acctgcgcct cctgaccgtg tccggcttcc
      361 tggtggacta cccgttcgcg aaccgtctct atccgggatc tctcgacgtg ctcgcgcgct
      421 tccgggagtg ggggccgacc gtgatcctct ccgacggcga cgtggtgttc cagccgcgca
      481 aggtggagcg ctccggcctg ggccgggcgg tggacggcgt gctgatctac gtgcacaagg
      541 agcgcgagct cgacgacgtg gagcggcggt atcccgccga gcactacgtg ctggtcgacg
      601 acaagctccg cgttctcgcc gcggtgaagc gcgcgtgggg cgcccgcgtg accaccgtgt
      661 tcccgcgcca ggggcactac gcgcacgatg cggaggccct cgccggctat ccccccgccg
      721 acctcaccgt cgagcgcatc ggcgacctca tgggccacga cctctccacg ctacgggcgc
      781 gcgccgcgga cggcgcggcc gccgggcccg ccaccgggag atgaccgcat ggcgatgtcg
      841 gccgagcaga tccgactcct cgacgcgtac tggcgggccg cgaactacct gaccgtcggc
      901 cagatctatc tccgggacaa cccgctgctg cgggagccgc tcacgcgcga gcacgtcaag
      961 ccgcggctcc tcggccactg gggcacctcg ccggggctct ccttcatcta cgcgcacctg
     1021 aaccgcctcg tgcgcgagac cgacgcggac gtgatctacc tggccggacc gggccacggc
     1081 gggcccgcga tcgtggccaa cgtatacctc gaaggcacgt actccgagat ctacccggat
     1141 gtctcgaggg acgcggcggg cctgcggcgc ctcttccggc agttctccac ccccgggggc
     1201 atcccgagcc acgtgagcgt gccgacgccc gggtccatcc acgagggcgg cgagctcggc
     1261 tacgtgctgg tccacgcctt cggcgccgcc ttcgacaacc cggagctgat cgtggccgca
     1321 gtggtgggcg acggcgaggc cgagaccggg ccgctggccg gcagctgggc cggcgtggac
     1381 ttcctgaacc cggcgcggga cggggcggtg ctgccgatcc tccacctcaa cggctacaag
     1441 atcggcgggc ccacgctgct cggccgcatg ggcgacgacg acatcgtgag cctgctgggc
     1501 ggccagggct gggagcccca cgttgtggcg ggcgacgatc cgggcgtgat gcacgcgcgc
     1561 ttcgcgcagg tgctcgacga ctgccacgcg cgcatccggg ccgtccgcgc tgaggcccgg
     1621 cggcccggcg tcggcgccgc ccgcgcgacg cgtccgcgct ggcccgcgat cgtgctccgc
     1681 acccccaagg gctggaccgg acccaaggtg gtggacggcc tgccggtcga gggcacgttc
     1741 cgcgcgcacc aggtgccggt ggaccgcgtg cgtgaggatc ccgcgcacct ggcgctgctc
     1801 gaggcctgga tgcgcagcta ccggccggag gagctcttcg acgagcgggg cggcctccgc
     1861 gccgagctgg ccgcgctcgc gcccacgggc gaccgccggc tcggcgcgaa cccgcacgcc
     1921 aacggcgggc gcgtgctcca gccgctcgac ctgcccgact tcgccgacta tgcgctgccg
     1981 gtgccgcagc cggccgcgga gctgcacggc tccacccgcc agctcggaaa gttcatgcgc
     2041 gacatcttca cgcgcaaccg agcggccgcc aacttccgcc tcttctgccc ggacgagacc
     2101 aactccaacc ggctcggcga tgtgttcgag gtggagaacc gatgctcggt gctcccgcgt
     2161 acggccgccg acgatcacgt ggcgccggac ggccgcgtga tggagatcct gagcgagcac
     2221 tgctgccagg gctggctcga gggctacctg ctcaccggcc gccacgggct cttcgcgagc
     2281 tacgaagcct tcgcgatggt ctcggcgtcc atgaccgtgc agcacgccaa gtggctgcag
     2341 gaggcggacc ggctgccgtg gcgggcgccg gtcgcctcgc tcaacatcct gctcacgtcc
     2401 accgcgtggc gccaggatca caacggcttc agccatcagg gcccggggct gatcgacgtg
     2461 gtgctctcga agaaggggac ggtatcgcgg atctacctgc cccccgacgc caacagcctg
     2521 ctctcggtgg ccgatcactg cttccgcagc cgcaactacg tgaacctgat cgtcatcgac
     2581 aagcagccgc agctgcagtg gctcgatctc gaagcggcgc gggcgcactg cgcgaggggc
     2641 gcctcggtct ggccctgggc gggcagtccg gacgacggac cgcccgacgt ggtgctggcg
     2701 gccgccggcg acgtgccgac gctcgaggtg atcgccgcgg cctggctgct gcgccgccgg
     2761 gcgccgtcgc tccgcgtgcg ggtggtgaac gtggtggacc tcatgacgct cttcgcgccg
     2821 cgctttcacc cgcacggcct caccgacgag gcgttcgtcg cgctgttcac cgcggcgacg
     2881 ccggtggtct tcgccttcca cggctaccag cgcgcggtcc acgagctggt ccacggccgc
     2941 ccgaacgccg agcgcttcca cgtgcgcggc ttcaacgagg agggcacgac caccactccc
     3001 ttcgacatgg tggtgcgcaa cgggatgagc cgctaccatc tctgcctcga ggcgctccgt
     3061 cgcgcgccgc agggcgaggc acggggcgtc gccctcgtcg cgcactgccg cgcgatgctc
     3121 gatcggcacc ccggctacat ccgcgagcac ttcgaggaca tgccggaagt gcgggactgg
     3181 cgctggagcg agccctgatg cccggcgccc cgatcatcct ctgcctgaac gctggctcgt
     3241 cgtcgctcaa gttctcggtc tgggacggcg acgagtgcct gggcgagggc gaggtggagg
     3301 agatcggtcg tccaggcgcg ttcgcctggc tcggaaaacc ggacggggtg ccgcgccggc
     3361 tgccgggccg atgggccgat cacggcgagg cggccgccgg ggtgttcgcg ctgctgggcg
     3421 agcacacgct gcccgcgccg gcgggcgtcg gccaccgcct cgtgcacggc gggcaatggc
     3481 acctggcgcc ggagcgggtg acgccgacgc tgctcgacga tctgcgcgcg ctccttccgc
     3541 tcgccccgct ccacctgcgg agcggcctcg cgggcgtgga ggcggtggcc gcgcgcttcc
     3601 ccgcgctgcc gccggccgtc tgcgtcgaca ccgcgtttca ccgggacctg ccggaggtgg
     3661 cccgccgcat ggccctgccg cacgagctga gcgatgcggc cgggatccgg cgcttcggct
     3721 tccacgggct gtcctacgag tacgtgatgg agcggctcgg cccggccggg cgcggccggg
     3781 tggtgatcgc gcacctcggg aacggagcga gcatggcggc ggtgcgggcc gggcggccag
     3841 tggacacgac catggggttg accccggccg gcgggttcat gatgggcacc cgcaccggcg
     3901 atctcgatcc gggcgtgctc ctctacctga tgcgggagca gggctacgac gcgtcacgcc
     3961 tcgaccggct ggtcaacaag gagtcggggc tgctcggggt gtccggcgcg agcggggaca
     4021 tgaagacgct gctggagcgg cgggagcgcg acggggccgc cgcgctggcg gtggcgatgt
     4081 tctgctacca ggcgcgcaag cagatcggcg ctttcgcggc ggcgatgggc ggcctcgaca
     4141 cgctggtgtt cacggggggc atcggggagc gggcggcgcc ggtgcgcgcg gagatctgtg
     4201 acgggctcgg ccacctgggc gtccgcctcg atccggcgcg gaacgcggcc cacgccgatc
     4261 cgctgagcgc cccgggcgac agctgcgcgg tgcgggtcgt gctcacccgt gaggacctga
     4321 tgatcgcgcg gcacagccgg gcggtcctgt tcgcggaacg gcgagaggag atacggtgat
     4381 cagccagagc gaggcgtggg cggcgctgcg gaagcatcac gggcagatcc gggacgtcca
     4441 tctgaggacg ctcttcgcgg aggacccgac gcgtggggag cggctcgcgc tggaggcggc
     4501 ctcactctat ctcgactact cgaagaaccg ggtcaccgac gagaccctgc ggctcctgct
     4561 cgcgctggcc gaggcggcgg gcctgcgggc gcgcatcgat gcgatgttct cgggcgagaa
     4621 gatcaacgtg accgagcagc gggcggtgct ccacgtggcc ctgcgcgcac cccggggcac
     4681 gcgcatcctg gtggacggcc aggacgtggt gcccggtgtg cacgcggtgc tcgaccgcat
     4741 ggccgacttc tccacccgcg tgcgcaccgg ggcctggacc ggcttcaccg ggaaggccat
     4801 ccgcaacgtg gtcaacatcg ggatcggcgg ctccgacctg gggccggcca tggcctacga
     4861 ggcgctgcgc cattacagcg accgccgtct cgggctgcgc ttcgtctcca acgtggacgg
     4921 caccgacttc atggaagc
//
LOCUS       DASUFA010000034         6181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_809448, whole genome shotgun sequence.
ACCESSION   DASUFA010000034 DASUFA010000000
VERSION     DASUFA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809448"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VFX87_01175"
     CDS             <1..947
                     /locus_tag="VFX87_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4993542.1"
                     /translation="HHRTSSAISQQIRKLERHLGVTLLERAGRGVRLTAAGEAALPGI
                     LKLGTETEALFAHLAELSGRPPATLRVAVSDYLGKALLVPVLRELLEERVPLRFEIAT
                     THSREGIRLVGLGEVDCAVVTGQEIPRGLEAERLFDQPFVWVGPRRPGRRVPLPDRLA
                     REPLLRLGAGSQGRRLLEDYLTRERIQPTSTIDVPSVSLLLAYVSGGLGVGLAPALAL
                     AELDRRRVRWERAAVPALPVTLVHRPGFRRGAAAGRFAARLIAECRARRSRLKGEVQR
                     RRADCRARPSRRRAERIQEMWEEPPASPGPRATRRAAR"
     gene            complement(907..2178)
                     /locus_tag="VFX87_01180"
     CDS             complement(907..2178)
                     /locus_tag="VFX87_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX4993543.1"
                     /translation="MDCGTRRSFLKGGALALFALGGPPRFLARAAFAQGTAARPKVLI
                     AIFQRGAVDGLSMIVPHGDPEYYAARGTIAIPRPAPGAADATIDLNGFFGLHPALAPL
                     RPIWDDRRLAIVHACGSPDTTRSHFDAQDYMESGTPGLKSTADGWLARGLGGMPAREP
                     TSPFRAVSLGPALPRILRGDVGAVAMHSVADFDVKDGGGAMMAGGPGARKGFESLYEQ
                     GVRDLLYGTGRETFEAVRMLKAAAPQRIAPANGAVYPRGRFGESLRQIAQLIKADLGL
                     EIAFADVGGWDTHAGQGNERGQLALRLKEFAEGLAALDRDLGPRMSDVVVLTMSEFGR
                     TVRENGNRGTDHGHATAMLALGGPVKGGRVYGRWPGLRREQLFDGRDLAVTTDFRCFF
                     TEVAVRHLGAPAGPLFPGFSAPPGAWPGALG"
     gene            complement(2181..3887)
                     /locus_tag="VFX87_01185"
     CDS             complement(2181..3887)
                     /locus_tag="VFX87_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX4993544.1"
                     /translation="MRRVWRGAGLIVLGLIGVACSSAPARTAAPPPAPASPPPVAVTR
                     PSSALSLPASSLGPDQRILHALNRLGYGPRPGDVERVRRMGLAAYIERQLDPQGIPDS
                     AVEQALAAYPVLTLSTVALLRDYPLPTPQARRRLAAGEMSRQEMIEMYPPERRPAVIT
                     AQMQAARITRAVASDRQLEEVMVDFWFNHFNVNAQKGAVRWMLPAYEREAIRPHALKR
                     FRDLVLATARHPAMLFYLDNWLSTRADLVVRGGPNAGRRMGLNENYARELMELHTLGV
                     DGGYTQQDVIEVARCFTGWTIDRPRQGGGFIFRPLAHDRGAKRVLGQVIPAGGGQQDG
                     ERVIDILARHEATARFIATKLARRFVSDDPPAALVERAAATFRRTDGDIRAVLATIFT
                     SPEFWSADAYRAKIKTPLEVVASAVRALDGRIAPGADAAGGGIALAREVGRLGEPLYQ
                     AQPPTGYPDRAEAWVNTGALLSRMNFALDLAHNRFRGARVDVAGFLTDTDRGQPALVL
                     DRLLAVVLHGEASAQTRAVLAAQLESPEITRTTAYDRVAKDTDVEKLAALVLGSPEFQ
                     RR"
     gene            complement(3959..4972)
                     /locus_tag="VFX87_01190"
     CDS             complement(3959..4972)
                     /locus_tag="VFX87_01190"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4993545.1"
                     /translation="MIAAVLRGPRSLALTTVASPTPGPSDAVIRVTAAGLCGTDYRIW
                     TGERPVRYPLVMGHEFVGTVAAIGSAVRNIAVGQPVAVEPNYSCGTCALCREGNRNLC
                     LSRVAVGIDVDGGFAQEATVPARCCWPAPPGLGSERLLMAEPLAVVVRAVGRAAPVAG
                     ETAAVLGVGSLGLLAVQVLRARGCRVLAVARTTRRLDLARELGAEAVALSGADDDAAA
                     ARAFSGREGVDLVVETAGTALAVEQAVNLCRPGGRVVLTGLPHEATSLNFFWVVRREL
                     RIIGSMIYQDEFPEAMRLLETGAVRTDRLLSRTFPLDRIDDAFAAHRSPDSIKVAVIP
                     PAG"
     gene            4995..>6181
                     /locus_tag="VFX87_01195"
     CDS             4995..>6181
                     /locus_tag="VFX87_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX4993546.1"
                     /translation="MDGPGASESRRPPRQRRLALLVLLAAVALAVAAYPLRTRWWGGL
                     VLAIAEAGIVGGLADWFAVTALFRHPLGLPIPHTAIIPANWELMAQRVGSMVGDRVLT
                     KDYVTHEISRFDIAELLARGAERVKPADLEAAVRAGARWAVEQVTPLATGDATLWLTR
                     LARANPIAPILATGIEIARKQGWDQRLIEAVAAALIDALDRPDFRATVADMVDEMLAG
                     YRARMGVYPRILMGLANTFGLIDRERIVTALHETLKKIADDPDDPLRGRLTETLATLP
                     ERLRTDRALAARVEAAKEELLASPAVARLLEDGAVGLRKIVIADLASAPSELVSWITA
                     QLDRARQTLAEDTALRQSLDRWVKERLTEAVDRYHDRIARFIERGVHALGPEGAVRLI
                     EEHA"
BASE COUNT          848 a         2285 c         2270 g          778 t
ORIGIN      
        1 tgcaccatcg cacgtcctcc gcgatcagcc agcagatccg caagctcgag cgccatctcg
       61 gcgtgacgct cctcgagcgc gcgggccgcg gcgtccggct caccgcagcg ggcgaggccg
      121 ccctgcccgg catcctcaag ctcggcaccg agacggaggc gctcttcgcc cacctcgccg
      181 agctgtcggg ccgcccgccc gcgactctgc gcgtcgcggt cagcgactac ctggggaaag
      241 ccctcctggt gccggtgctg cgcgagctgc tggaagagcg ggtgccgctc cgcttcgaga
      301 tcgccaccac ccactcgcgc gagggcatcc ggctggtggg cctcggcgag gtggactgcg
      361 cggtggtgac cggccaggag atcccgcgcg ggctcgaggc cgagcgcctc ttcgaccagc
      421 ccttcgtctg ggtcggtccg cgccggcccg ggagacgcgt gcccctgccc gatcgcctcg
      481 cccgcgagcc cctgctccga ctcggggcgg ggagccaggg ccgacggctg ctggaagact
      541 acctgacgcg tgagcgcatc cagcccacct ccaccatcga cgtgccgagc gtgtccctcc
      601 tgctcgccta cgtctccgga ggcctcgggg tgggcctggc ccccgccctc gccctggccg
      661 agctggatcg ccgccgggtc aggtgggagc gggcggcggt gccagcgctg ccggtcacgc
      721 tggtccaccg gcccgggttt cgccgcggcg ccgcggccgg ccgcttcgcg gccaggctca
      781 tcgccgagtg cagggccagg cgcagccgcc tgaagggcga ggtgcagcga aggcgagccg
      841 attgcagggc gaggcccagc cgaaggcgag cggagcgaat acaagaaatg tgggaagagc
      901 cgccggctag cccagggccc cgggccacgc gccgggcggc gcgctgaagc ccgggaacag
      961 cggcccggcc ggcgcgccca gatggcgcac ggccacctcg gtgaagaagc accggaagtc
     1021 ggtggtcacc gccaggtcgc ggccatcgaa gagctgctcg cgccggaggc ccggccagcg
     1081 cccgtagacc cggccgccct tgaccggccc gcccagcgcc agcatcgcgg tggcgtgacc
     1141 gtgatcggtg ccgcggttgc cgttctcgcg tacggtgcgg ccgaactccg acatcgtgag
     1201 cacgacgaca tcgctcatcc ggggcccgag atcccgatca agcgcggcaa ggccttcggc
     1261 gaactccttg agacgcaggg cgagctgccc gcgctcattg ccctgccccg cgtgggtgtc
     1321 ccagccgccc acgtccgcga aagcgatctc gaggcccagg tcggccttga tgagctgggc
     1381 gatctgccgc aggctctcgc cgaagcgccc gcgcggatac acggcgccgt tcgccggcgc
     1441 gatccgctgg ggcgccgccg ccttgagcat cctgacggcc tcgaaggtct cgcggccggt
     1501 cccgtagagg agatcgcgca cgccctgctc gtagagcgac tcgaagccct tccgcgcgcc
     1561 cgggcccccg gccatcatgg ccccgccgcc gtccttgacg tcgaagtcgg ccacgctgtg
     1621 catcgccacc gcgcccacgt ccccccgcag gatccgcggc agggccggcc ccagggagac
     1681 agcccggaaa ggcgaggtcg gctcgcgggc cggcatcccg cccagccccc gtgcgagcca
     1741 gccgtcggcg gtgctcttga ggcccggcgt ccccgactcc atgtagtcct gcgcgtcgaa
     1801 gtgggagcgg gtcgtgtcgg gcgagccgca cgcgtgcacg atggcgaggc gccggtcgtc
     1861 ccagatcggg cggagcggcg cgagcgccgg gtgcaggccg aagaagccgt tcaggtcgat
     1921 ggtcgcgtcg gccgcgcccg gggccggacg ggggatcgcg atggtgccgc gcgcggcgta
     1981 gtactcgggg tcgccgtgcg ggacgatcat gctgaggccg tccaccgcgc cccgctggaa
     2041 gatggcgatc aggaccttcg gccgggccgc ggtcccctgc gcgaaggccg cgcgtgcgag
     2101 gaaccggggc gggccgccca gcgcgaacag ggcgagcgcc ccgcccttca ggaacgatcg
     2161 ccgcgtgccg cagtccatgc ctatcgccgc tggaactcgg gggagcccag gaccagcgcg
     2221 gccagcttct ccacgtcggt gtccttggcc acgcggtcat aggccgtggt ccgggtgatc
     2281 tccgggctct cgagctgggc cgccagcacc gcgcgggtct gggccgacgc ctcgccgtgg
     2341 aggacgaccg ccaggaggcg atcgagcacc agcgcgggct ggccgcgatc cgtgtcggtc
     2401 aggaagccgg ccacgtccac ccgggcgcca cggaagcggt tgtgggccag gtcgagcgcg
     2461 aagttcatcc gagagagcaa cgcgcccgtg ttcacccagg cctccgcgcg gtccgggtag
     2521 cccgtgggcg gctgggcctg gtagagcggc tccccgagcc tgccgacttc acgggccagc
     2581 gcgatcccgc cacccgcggc gtcggcgccc ggggcgatgc ggccgtcgag cgcgcgcacc
     2641 gcgctggcca cgacctccag cggggtcttg atcttggcgc ggtaggcatc cgccgaccag
     2701 aactccggcg aggtgaagat cgtcgccagg accgcgcgga tatccccatc cgttctccgg
     2761 aacgtcgccg cggcgcgctc caccagggcc gcaggcggat cgtcgctcac gaaccggcgg
     2821 gcgagcttgg tcgcgatgaa gcgcgcggtc gcctcgtgcc gcgccaggat gtcgatcacg
     2881 cgctcgccgt cctgctggcc gccgccggcc gggatcacct gcccgagcac gcgcttggcg
     2941 ccgcggtcgt gcgccagcgg gcggaagatg aagccgccgc cctgccgggg tcggtcgatg
     3001 gtccagccgg tgaagcagcg cgcgacctcg atcacgtcct gctgggtgta gccgccgtcc
     3061 acgcccaggg tgtgcagctc catgagctcg cgggcgtaat tctcgttgag ccccatgcgc
     3121 cggcccgcgt tggggccgcc gcgcaccacc aggtcggcgc gcgtgctgag ccagttgtcg
     3181 aggtagaaga gcatcgcggg gtgacgcgcg gtggccagca cgagatcgcg gaatcgcttg
     3241 agcgcatgcg gccggatcgc ctcgcgctcg taggccggca gcatccagcg caccgcgccc
     3301 ttctgcgcgt tgacgttgaa gtggttgaac cagaagtcca ccatcacttc ctcgagctgc
     3361 cggtcgctcg cgaccgcccg cgtgatccgg gcggcctgca tctgcgcggt gatgacggcg
     3421 ggtcggcgct cgggcggata catctcgatc atctcctggc ggctcatttc gccggcggcc
     3481 aggcgccgcc gcgcctgcgg cgtgggcagc gggtagtcgc ggaggagcgc gacggtgctg
     3541 agggtaagta ccggatacgc cgcgagcgcc tgctcgaccg cggagtccgg aatgccctgc
     3601 gggtcgagct ggcgctcgat atatgcagcg aggcccatcc gccgcacccg ctcgacgtcg
     3661 cccgggcgcg gcccgtagcc gaggcggttg agcgcgtgga ggatccgctg gtccgggccg
     3721 agggaagagg cgggcaggct gagcgcggac gaggggcgcg tgaccgccac gggcggaggc
     3781 gaggccggcg cgggcggcgg ggcggccgtc cgagccggcg ccgaggagca cgccaccccg
     3841 atcagcccca gcacgatcaa gcccgcccca cgccagactc gccgcatcgc gaccctccga
     3901 gacgtacaga cgccgccccg tatcctaatg tttacggggc ggcgggaaga ggcagggcct
     3961 agccggccgg cgggatgacc gcgaccttga tcgagtccgg cgaccgatgg gccgcgaagg
     4021 cgtcgtcgat gcggtcgagc gggaacgtcc gggagaggag ccggtccgtt cggaccgcgc
     4081 ccgtctcgag gagccgcatc gcctccggga actcgtcctg atagatcatc gagccgatga
     4141 tccgcagctc gcggcgcacc acccagaaga aattgagcga ggtcgcctcg tgcggcaacc
     4201 cggtgagcac cacgcgcccg ccggggcggc acaggttgac ggcctgctcg acggccaggg
     4261 ccgtgccggc ggtctcgacc accagatcca cgccctcgcg cccggaaaag gcgcgcgccg
     4321 ccgccgcgtc gtcgtcggct cccgagagcg cgaccgcctc cgcgcccagt tcgcgcgcca
     4381 gatcaagccg tctggtggtc cgcgccaccg ccagcacgcg gcagccgcgc gcccggagca
     4441 cctggaccgc gagcaacccg agcgagccca ccccgagcac cgccgcggtc tccccggcca
     4501 cgggcgcggc gcggccgacc gcgcgcacca ccaccgcgag cggctcggcc atgagcaggc
     4561 gctcgctacc gaggccgggg ggcgccggcc agcagcagcg ggcgggcacg gtcgcctcct
     4621 gggcgaatcc gccgtccacg tcgatgccga ccgccacgcg cgagaggcac aggttgcggt
     4681 tgccctcgcg gcagagcgcg cacgtcccgc aggagtagtt gggctccacc gccaccggct
     4741 ggccgaccgc gatattccgg accgcgctcc cgatcgcggc caccgtgccg acgaactcgt
     4801 gccccatcac gagcggatag cgcaccggcc gctcccccgt ccagatgcgg tagtcggtac
     4861 cgcagagtcc ggccgcggtg acccggatga ccgcgtcgct cggacccggc gtgggtgacg
     4921 cgacggtcgt gagggcgagc gagcggggac cacgaaggac ggcggcgatc atgccgcgat
     4981 gatagcattc gtgcgtggac ggccccggcg cttccgaatc ccggcgtccg ccccgccagc
     5041 ggcgtctcgc cctgctcgtg ctgctggcgg cggtggccct ggcggtggcg gcgtacccct
     5101 tgcgcacccg gtggtggggc ggcctcgtcc tggccatcgc ggaggccggc atcgtgggcg
     5161 gcctcgccga ctggtttgcg gtcaccgcgc tcttccggca cccgctcggt ctgccgatcc
     5221 cccacaccgc catcattccg gccaactggg agctcatggc ccagcgcgtg ggaagcatgg
     5281 taggcgatcg ggtgctcacc aaggactacg tcacgcacga gatctcgcgg ttcgacatcg
     5341 ccgagctgct ggcccgcggg gccgagcgcg tcaagccggc cgatctggag gcggcggtcc
     5401 gcgcgggggc gcgctgggcg gtggagcagg tcacaccgtt ggcgaccggc gacgccacgc
     5461 tctggctcac gcgcctggcc cgggccaatc cgatcgcgcc gatcctggcc accggcatcg
     5521 agatcgcgcg caagcagggc tgggatcagc ggctcatcga ggcggtggcg gcggccctga
     5581 tcgacgcgct cgaccgcccg gactttcgcg ccacggtcgc cgacatggtg gacgagatgc
     5641 tggccggcta tcgcgcccgg atgggcgtct atccgcgcat cctcatgggc ctggcgaata
     5701 ccttcgggct catcgatcgc gaacggatcg tcaccgccct gcacgagacg ctcaagaaga
     5761 tcgcggacga ccccgacgac cccctgcgcg gccggctcac cgagacgctc gccacgctgc
     5821 ccgagcggct ccgcaccgac cgggccctgg ccgcgcgcgt cgaggcggcc aaggaggaac
     5881 tgctggcgag cccggcggtg gcgcgactgc tggaggacgg ggcggtcggg ctccgcaaga
     5941 tcgtgatcgc ggatctggcc tccgcgccgt ccgagctggt gagctggatc accgcgcagc
     6001 tcgaccgcgc ccggcaaacc ctggccgagg acaccgcgct acggcagtcg ctcgatcgct
     6061 gggtcaagga gcggctcacc gaggcggtgg accgttacca cgaccgcatc gcgcgcttca
     6121 tcgagcgggg tgtccacgcg ctcgggccgg agggcgcggt gcgcctcatc gaggagcacg
     6181 c
//
LOCUS       DASUFA010000035        12990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1496699, whole genome shotgun sequence.
ACCESSION   DASUFA010000035 DASUFA010000000
VERSION     DASUFA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496699"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..568)
                     /locus_tag="VFX87_01200"
     CDS             complement(176..568)
                     /locus_tag="VFX87_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4993547.1"
                     /translation="MAESELHRKGNEIRRKLLGDKYVERVNATTYKDPVMRTFIDVAT
                     ENVFGALWTRPGLDLKTRTLVCVVSDAATGREPELRIHLRMALRQGWTETELIETLIH
                     MSGYVGVPIIREAMIAAGQVFAEVKAEG"
     gene            complement(587..901)
                     /locus_tag="VFX87_01205"
     CDS             complement(587..901)
                     /locus_tag="VFX87_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993548.1"
                     /translation="MGSAFIRVAGCDLCRAARITPWFHEDDICWIAECEICEVPMVVW
                     RYHGTEPPAEHLTHMHARLREIATQQLGDCYIDDHMRNIPDHYHAHGRPRGGFFGPGA
                     RR"
     gene            complement(929..1354)
                     /locus_tag="VFX87_01210"
     CDS             complement(929..1354)
                     /locus_tag="VFX87_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX4993549.1"
                     /translation="MRGQPGQESSVFRKPTAPPPDRAAQRRGPEARSDRSVVIPARVA
                     SSLSRTVREQLAYLTPDQQRAFLWRYRAQAKSPVVALAFLPLGWHYLYLRKRGLQVLF
                     WITLGGLAAWWVLDIFRIRKLVRDYNRRVAMHVMRALWG"
     gene            complement(1497..2339)
                     /locus_tag="VFX87_01215"
     CDS             complement(1497..2339)
                     /locus_tag="VFX87_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993550.1"
                     /translation="MARIVAAMAMTHSPGLTGWFDRAPEDQRLEARRALDEMRGRLRA
                     ARPDVIVLFSNDHLLNWPINNTPEYTVGIGAEHVGPADWYDEWLALAKYRIPGHPTLA
                     RYLVNEGARRRLAFSYLREMQFDDGVSVPMHYLNPEGDIPLVPVTMNCTVPPIPAPAR
                     AYEVGTIVREMLREHPGGERVAVVATGGLSHEPGGPRYFWVDEAFDRWFLGLLKQGDH
                     AALLRECTLERMEAAGSGGTAELLAWILALAFTSGPAEVLAYMPAVAWRSGTGMVVWP
                     EVKN"
     gene            complement(2344..2691)
                     /locus_tag="VFX87_01220"
     CDS             complement(2344..2691)
                     /locus_tag="VFX87_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993551.1"
                     /translation="MGNVERCDKTLPVNEMIFYVRRDAVLRARWLSDLEGLAREFGLS
                     REEYEAVRDKDVRRLNEMGVHQYYIPQILRLFYGASANSNSHPALEAYRRAYPDEAAA
                     AERLLAPPTRREG"
     gene            complement(2684..3061)
                     /locus_tag="VFX87_01225"
     CDS             complement(2684..3061)
                     /locus_tag="VFX87_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993552.1"
                     /translation="MEETVTSRVTGVAQVFDLHALKAFAPDKRVRKMLFKTDQLWSEI
                     ACYEPGQSTVMHAHPKEEEAIFVLEGTAHMSIDGEEVVVEAGSIVKFPRSVAHDVRNL
                     GPERCVIMFLKVNPRALSARADG"
     gene            3171..4568
                     /locus_tag="VFX87_01230"
     CDS             3171..4568
                     /locus_tag="VFX87_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX4993553.1"
                     /translation="MPQPPASSSSVARRLARFVSGFSLDTVPPAVSAQATLLALDTLG
                     SCLASSRQAFGRAVTEAAGRLGGPPESVVIGSKARIGAAAAALANGTLAHGLDFDDTR
                     EDAIVHTGCVAVTTALAVGGAVEASGHAALEALLAGVEVMCRIGLAVPGRFHARHYHP
                     TALTGSFAAAATAGRLYGLTEDQLVHAFGICGSQASGIIEYLADGSWTKRLHPGWAAH
                     AGVTAALLARAGFTGPEAVFEGAHGFYAAFAGGHEPARLDALLESLGRSWEICNLTFK
                     PYPCGSIAHPYMDCALRLRDRHRPRPEEIAEVRCRTAEGPVPRLWEPLAAKHRPRNGY
                     AAKFSLPYLLASILVNGRATLADFTDEAVRDETLRGVAAKIYYDIDPTIDYPRHFVGH
                     VAVRLADGRVLEERQDHPRGGPDFPMPREELIAKFLANAAEAVPAEQASRVVALVDAL
                     AAQPRLAPLMDALTT"
     gene            4579..5601
                     /locus_tag="VFX87_01235"
     CDS             4579..5601
                     /locus_tag="VFX87_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4993554.1"
                     /translation="MRDRPLALVLLISALIVSAAPATAQDPRLEAARKEGKVVWYTSL
                     ALTSSEKVAKLFETAYPGVKVEVHRTGSQRVLQRMMQELQANIKNVDVVHTSDAGHYV
                     LLKEKQLLMRYTPAGVDRFAAGFKDRDGFHYGLRATVNVIAYNTQKITAAEAPRTWKD
                     LLDPKWRGRLVTAHPGYSGVIATHVLALVHLHGWDYFKALAQNKPMLVQSAVDPAGIV
                     ASGERPVAVNGGDYTFYQVKKKGNPVEIVFPKEGVPLVVSPSAITSFAPHPNAARLFT
                     DFIFSRDLQQVLADTEGLYTGHPDVKYPADRPKLGDLKLLQVDPEELEKRNEEIKTRF
                     VEFFGA"
     gene            5612..7333
                     /locus_tag="VFX87_01240"
     CDS             5612..7333
                     /locus_tag="VFX87_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4993555.1"
                     /translation="MAEASAASLRPGRLEGLDRAAPVWLLAAAALVMLILLPMGWLAY
                     ISVTGPRGFTLAHYARVLGDPPLQKALWNTVVLAFWSGVVALAIGAPMAWLSARADLP
                     GRRIIQSLIMASFVTPPFLGAFAWVMLAGPNAGYLNQLYRGLTGAEAPLLNIFSMPGL
                     IFVVAIYTFPYVYIMIANTLGLIASDLEDAAAILGAGRLQVARTITLPMVLPAILSGF
                     ILAVLQALALFGSPAILALPAGFHTVTTQMWSFFQYPPRAELAAAFSIPLLLATALLL
                     LVQKKLLGRRGYAAVGGKGGQRRTIRLGPWRYPALAGCLAVLACAVFLPYGILLKAAF
                     ARAWAQPLTRGNFTLENWSFTFLHSATQSAIVNTLELGVLTACVGASLATLLAYVTNR
                     RLVMGHQVLGFLAVAPVVIPGVVLAVALFVAYTRPPFLLYGTLWILFIAYLTKEMPVG
                     YSQADATMRGIHPELEEAGRILGAGRLRVLREITAPLARSGIIATWCFVFIGVIRELS
                     ASIILFTPNTKVMSVVMFDLKEEGQFGAIAVLGVFMLVMTFATVALMQTLLGRDVLGA
                     RSSSDSG"
     gene            7338..8420
                     /locus_tag="VFX87_01245"
     CDS             7338..8420
                     /locus_tag="VFX87_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4993556.1"
                     /translation="MPGITITGLTKRFGDVAAVAGLDLTVRPGELVALLGPSGCGKTT
                     TLRLVAGFMAPDAGEIRVGERVLSSPGAVIPPERRRMAMIFQSYALWPHMTVAQNVAY
                     GLRFTKTPRAQRDGKIDEILRAVQLAGYGARYPGELSGGQQQRVAVARALVVEPEILL
                     LDEPLSNLDASLREEMRFEIRRLHERFGITTLYVTHDQAEAMVISDRAAVIRNGRVEQ
                     IGAPQELFERPRTRFVAEFIGKTNLIDTVADGAGSVARGRLRLRVAADGLTPGTPAVV
                     SIRPHLIALGPRADARMPAAGANALGGTVLRASYLGDTVDYQVALEESDVVLRVTGPA
                     PARARAGDPVSVTVPAESCVLLPAES"
     gene            complement(8427..9545)
                     /locus_tag="VFX87_01250"
     CDS             complement(8427..9545)
                     /locus_tag="VFX87_01250"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX4993557.1"
                     /translation="MAPPRAPFRADHVGSLLRPPALKALRLEREAGRASAAELAAAED
                     AAIREGVRLQEEAGLLGVTDGEMRRKSWHMDFLTRLGGLASEGKQLPVTFHGRAGDVN
                     FTRPDLRIASRVGRPAPIFVDAFRFLRSTTSRTAKLTIPSPCMLYSQVGRANIDRAVY
                     PDLDGFVEDTAAAYRAEIADLYAAGCRYLQLDDVSLAYLCDEDMRAQGRARGEDPDEV
                     LALSVKLIQATLRDRPKDLVVCTHLCRGNFRSSWRAQGGYEKVAEAIFNQMPYDGFFL
                     EFDDARSGDFSPLRHAPKHIRVVLGLVTTKVGVLERRADLKRRLDEASRYIALDQLCV
                     SPQCGFSSTIEGNEVAVEHQKAKLALCVELADEVWGGL"
     gene            complement(9548..10525)
                     /locus_tag="VFX87_01255"
     CDS             complement(9548..10525)
                     /locus_tag="VFX87_01255"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4993558.1"
                     /translation="MKTVAIVGAGAIGTFLGTYLARSGSDVSAVARGTTAAVLRAHGF
                     RFEEGGVVLTAPVRVAEEPAALGPQDLVVLAVKGPSLPGLASTIRPLLGPDTVALTAM
                     NGVPWWFFYGLGGPHEGAAVRAVDPDGRVAAAIPVRHVIGCVVHATCSVQEPGLVKHG
                     FGRGLIIGEPGGGVSGRVSALADRLAAAGFETTVSRRIQADIWYKLWGNMTMNPMSAL
                     TGATCDRLLDDPLLERFTLAIMAEAAAIGDRIGCPIHQSGEDRNAVTRKLGAFKTSML
                     QDVEAGRPLEIDGLLTAVSEIGRRVNVPTPSTDALLGLTRVFAAGRGLY"
     gene            complement(10522..11508)
                     /locus_tag="VFX87_01260"
     CDS             complement(10522..11508)
                     /locus_tag="VFX87_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993559.1"
                     /translation="MDRRAFVAGIAFGILAAPLSARAQQRAPKPVRVGVLRAAPDAPI
                     FRQNAEQFLQPLRDSGFTEGTNLVIDFRVRAGRTEDMAVLAGELVRLGMDGILAVGPA
                     AVRAAAGTTRSIPIVAVDLESDPVAAGFATSLGRPGKNVTGVFLDFPELSGKWIQLLR
                     ELVPKLSRIAAVWDPSTPSNLLKGAEAAARALRVEIFALEALTAEDFAPAFRSAAEKR
                     ADALMVLSSPVFYSARPQVVEGAARHRMPTIMPFPEFADHGGLISYGPNVPAMFRQAG
                     EVMVKVLRGTRPGDIPLELPARFELSVNRRTAASLGLTVPQSLLVRADRVIE"
     gene            complement(11518..12612)
                     /locus_tag="VFX87_01265"
     CDS             complement(11518..12612)
                     /locus_tag="VFX87_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993560.1"
                     /translation="MSEPARILVVDDTPMNVKLLEGVLAGHGYAVTTASSGAAALERV
                     RAEQPDLVLLDVVMPEMSGYEVCRRLRADEATRLLPIVMVTALDPTTERVKGIEVGAD
                     DFLSKPINQPELLARVRSLLRIRALHRELEELNRTLEQRVRDQVAQLERLGRLRRFFS
                     PQLAELIVAGDTEDPLKSHRREITVVFLDLRGFTAFAETAEPEEVMTVLREYHGEMGR
                     LILAAEGTLERFAGDGMMIFFNDPVEVSDPAVRAVRMAATMRERIAGLTEAWRKRGFD
                     LDFGVGIAQGYATIGAVGFEGRLDYGAIGTVTNLASRLCGEAKPGQILLSQRVAGGVE
                     DIAEVEGVGELTLKGFQRPVKAFNVVRLKE"
     gene            complement(12609..>12990)
                     /locus_tag="VFX87_01270"
     CDS             complement(12609..>12990)
                     /locus_tag="VFX87_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993561.1"
                     /translation="ILIVEDNEKNRKLERDILQFHGYRTAEADNAEDGIALAQATPPA
                     LVLMDIQLPRMNGIEALQVLRSDARTRAIPVIAVTASAMTQDRQKIMAAGFDGYQAKP
                     IDVTQFVAAVRSMLDRPAKGEGPR"
BASE COUNT         1845 a         4793 c         4442 g         1910 t
ORIGIN      
        1 agccggtcga tctcgagagc atgccggagg cgaaccgacg gcgcatcctg accttcagac
       61 ggatgcggga gatcatggag ggccgttgag gccggcacgg cgccggactc gccgccgtcg
      121 cccccgaagc tcgccggccg gcggcggcgg gtcgaccgga ccggagcgcc gagcctcagc
      181 cctcggcctt cacctccgcg aacacctggc ccgccgcgat catcgcctcg cggatgatgg
      241 gcacgcccac gtagcccgac atgtggatca gggtctcgat cagctcggtc tcggtccagc
      301 cctggcgcag cgccatgcgc aggtggatgc gcagctcggg ctcgcggccg gtggccgcgt
      361 cggagaccac gcacacgagc gtgcgcgtct tgagatcgag gcccggccgc gtccagagcg
      421 cgccgaagac gttctccgtc gccacgtcga tgaacgtgcg catgaccgga tccttgtagg
      481 tggtcgcgtt cacgcgctcc acgtacttgt cgcccagcag cttgcggcgg atctcgttgc
      541 ccttccggtg gagctcgctc tctgccatgg tcgtctccag gtcgcgctag cgccgggcgc
      601 cgggaccgaa gaacccgccc cggggccggc cgtgggcgtg gtagtggtcg gggatgttgc
      661 gcatgtggtc gtcgatgtag cagtcgccga gctgctgggt ggcgatctcg cgcaggcgtg
      721 cgtgcatgtg ggtgagatgc tcggcgggcg gctcggtgcc gtggtagcgc cacaccacca
      781 tgggcacctc gcagatctcg cactcggcga tccagcagat gtcgtcttcg tggaaccagg
      841 gggtgatgcg cgcggcgcga cagagatcgc agcccgcgac gcggatgaag gcggaaccca
      901 tgggggaccg acggaccgac cggctaccct acccccagag cgcccgcatc acgtgcatcg
      961 ccacgcgccg gttgtaatcc cgcacgagct tgcggatgcg gaagatgtcc agcacccacc
     1021 acgccgcgag cccgcccagg gtgatccaga acaggacctg gaggccgcgc ttgcgcaggt
     1081 agaggtagtg ccagccgaga ggcaggaatg cgagcgcgac gaccggcgac ttcgcctggg
     1141 cccggtagcg ccacaggaag gcacgctgct gatccggcgt gaggtacgcg agctgctcgc
     1201 gcaccgtccg cgagagggac gaggccaccc gcgcgggaat caccaccgac cgatcgctcc
     1261 gcgcctcggg gccacgccgc tgtgcggcac gatcgggtgg cggcgcggtc ggcttgcgga
     1321 acaccgacga ctcctgcccc ggctgtcccc tcatgacact cgttcccccg ggcccggaag
     1381 gagcaagaag agcgccagcg ggacgggtga gacgcggctt ggacttagcg gtggttcgct
     1441 gatgaacgat tctcacgtgt cactccggtc gtccacggga ggacgcttgc tgggtgtcaa
     1501 tttttgacct ccggccagac caccatgccg gtacccgacc gccaggccac cgcaggcatg
     1561 taggcgagca cctcggcggg gccgctcgtg aacgcgagcg cgaggatcca ggcgagcaat
     1621 tccgcggtgc cgccggagcc cgcggcctcc atccgctcga gcgtgcactc gcgcagcagc
     1681 gccgcgtgat cgccctgctt gagcaggccg aggaaccacc ggtcgaacgc ctcgtcgacc
     1741 cagaagtagc gcgggccgcc cggctcgtgc gagagcccgc cggtggcgac caccgcgacg
     1801 cgctcgccgc ccgggtgctc ccggagcatc tcgcggacga tcgtccccac ctcgtaggcg
     1861 cgcgcgggcg ccgggatggg tgggaccgtg cagttcatcg tcaccggcac caaggggatg
     1921 tcgccctcgg ggttcagata gtgcatcggc accgacaccc cgtcgtcgaa ctgcatctcg
     1981 cgcaggtacg agaacgcgag ccgccgccgc gcgccctcgt tgaccaggta gcgggcgagc
     2041 gttggatgcc cggggatccg gtacttcgcg agcgcgagcc attcgtcgta ccagtccgcg
     2101 ggccccacgt gctcggcccc gatccccacc gtgtactcgg gcgtgttgtt gatcggccag
     2161 ttgagcaggt gatcgttgct gaacagcacg atcacgtcgg gccgggcggc gcgcagccgc
     2221 ccccgcatct cgtccagcgc gcgccgggcc tcgagccgct ggtcctcggg ggcccggtcg
     2281 aaccagccgg tgagcccggg cgagtgcgtc atcgccatcg cggccacgat gcgcgccatc
     2341 cgctcagccc tcccgccgcg tcggcggggc gagcagccgc tccgcggcgg cggcctcgtc
     2401 cgggtaggcc cgccggtagg cttcgagcgc cgggtggcta ttgctgttgg cggaggcccc
     2461 gtagaagagc cggaggatct gggggatgta gtactggtgc acccccatct cgttgagccg
     2521 ccggacatcc ttgtcgcgga ccgcctcgta ctcctcgcgg ctcaggccga actcgcgcgc
     2581 gagcccttcg aggtcgctga gccagcgggc ccggaggacc gcgtcccgcc ggacgtagaa
     2641 gatcatctcg ttcaccggca gggtcttgtc gcagcgctcg acgttaccca tcggccctcg
     2701 cggacagcgc cctgggattg accttgagga acatgatgac acagcgctcg ggacccaggt
     2761 tccggacgtc gtgcgcgacg ctccggggaa acttcacgat ggagcccgcc tcgaccacca
     2821 cttcctcgcc gtcgatgctc atgtgggcgg tcccctccag cacgaagatc gcctcctctt
     2881 ccttcggatg cgcgtgcatc acggtggact ggcccggctc gtagcaggcg atctccgacc
     2941 agagctggtc ggtcttgaag agcatcttcc gcacgcgctt gtcgggggcg aaagccttca
     3001 gcgcgtgtag atcgaagacc tgcgctacac cagtcacacg cgaggtcacc gtctcttcca
     3061 ttacgcccgg atcatagcgc gcgcgcgcgg cgtccgctac cggcccgccg tgcgcgcgga
     3121 acttcgtcgt tgacatcccc cgacccgttc agtagaacaa gccgacggcc gtgccacagc
     3181 ctcccgcctc gtcctcctcg gtcgctcggc gcctcgcgcg attcgtgtcc ggcttctcgc
     3241 tcgacacggt accgcccgcc gtctccgcgc aagcgacgct gctggcgctc gacaccctcg
     3301 gaagctgcct ggcgtcttcg cgacaggcgt tcggccgggc ggtcaccgag gccgcgggcc
     3361 gcctcggggg cccgccggag agcgtggtga tcggcagcaa ggcgcggatc ggcgccgccg
     3421 ccgccgcgct cgccaacggc acgctggccc acgggctcga tttcgacgac acgcgcgagg
     3481 acgccatcgt ccacaccggc tgcgtggcgg tgacgaccgc cctggcggtg ggcggagcgg
     3541 tggaggcttc cggccacgcc gcgctcgaag ccttgctcgc cggggtggag gtgatgtgcc
     3601 ggatcggcct cgcggtgccc ggccgcttcc acgcgcggca ctatcacccc accgcgctca
     3661 ccggcagctt cgcggccgcc gccacggcgg gccggctcta cggattgacg gaggaccagc
     3721 tcgtccacgc cttcggcatc tgcggcagcc aggcctccgg catcatcgag tacctggccg
     3781 acggctcctg gaccaagcgc ctccatcccg gctgggccgc ccacgccggc gtgaccgcgg
     3841 cactgctcgc gcgcgccggc ttcaccggcc cggaggcggt cttcgagggc gcgcacggct
     3901 tctacgcggc cttcgcgggc ggccacgagc ccgcccggct ggacgcgctg ctcgagagcc
     3961 tggggcggtc gtgggagatc tgcaacctca ccttcaagcc ctacccctgc ggctccatcg
     4021 cgcatccgta catggactgc gcgctccggc tgcgggaccg ccaccggccg cgccccgagg
     4081 agatcgccga ggtgcgctgc cgcaccgcgg agggcccggt cccgcgcctc tgggagccgc
     4141 tcgcggcgaa gcaccggccg aggaacggct acgcggcgaa gttcagcctg ccgtatctgc
     4201 tcgcctcgat cctggtcaac ggccgggcca ccctcgccga cttcaccgac gaggcggtcc
     4261 gggacgagac cctgcgcggc gtggccgcca agatctacta cgacatcgac ccgaccatcg
     4321 attacccgcg ccacttcgtc gggcacgtgg cggtgcgcct ggccgacggg cgcgtgctgg
     4381 aggagcggca ggaccacccg cgcggcgggc ccgatttccc gatgccgcgc gaggagctca
     4441 tcgccaagtt cctcgccaac gccgcggagg cggttcccgc cgagcaggcc tcgcgcgtgg
     4501 tcgcgctcgt ggacgccctg gccgcgcagc cgcggctcgc ccccctgatg gacgccctca
     4561 ccacctagag gagccatgat gagagaccgc cccctcgcgc tcgtgctgct gatctcggcc
     4621 ctgatcgttt ccgcggctcc cgccaccgcg caggatccgc ggctcgaggc cgcgcgcaag
     4681 gaaggcaagg tcgtctggta cacctcgctc gcgctgacca gctcggagaa ggtggccaag
     4741 ctcttcgaga ccgcgtaccc gggcgtgaag gtcgaggtgc accggaccgg ctcgcagcgg
     4801 gtcctgcagc ggatgatgca ggagctccag gccaatatca agaacgtgga cgtcgtccac
     4861 acgtccgacg ccggccacta cgtgctgctg aaggagaagc agctcctgat gcgctacacc
     4921 ccggccgggg tggaccgctt cgcggccggc ttcaaggacc gcgacggctt ccactatggc
     4981 ctgcgcgcca ccgtgaacgt gatcgcctac aacacgcaga agatcaccgc cgcggaggcg
     5041 ccccgcacct ggaaggacct gctcgatccg aagtggcgcg gccggctcgt caccgcccat
     5101 cccggctaca gcggggtgat cgccacccac gtcctggccc tcgtgcacct gcacggctgg
     5161 gactacttca aggcgctggc ccagaacaag ccgatgctgg tgcaatcggc ggtggatccc
     5221 gccggcatcg tggcctccgg cgagcggccg gtcgcggtca acggcgggga ctacaccttc
     5281 taccaggtga agaagaaggg caatccggtg gagatcgtct tccccaaaga gggcgtgccc
     5341 ctggtggtct ccccgagcgc gatcaccagc ttcgcgccgc atcccaacgc ggcccggctc
     5401 ttcaccgact tcatcttcag ccgcgacctg cagcaggttc tggccgacac cgagggcctc
     5461 tacaccgggc atccggacgt gaagtaccct gccgaccggc cgaagctcgg ggacctcaag
     5521 ctcctgcagg tggatccgga agagctcgag aagcggaacg aagagatcaa gacgcgcttc
     5581 gtggagttct tcggcgccta gcgcgcccgt catggccgag gcgtcggcgg catcgctcag
     5641 gcccggccgt ctcgaagggc tcgaccgcgc cgccccggtc tggctgctcg cggcggccgc
     5701 gctggtcatg ctgatcctgc tgccgatggg ctggctcgcc tacatcagcg tgaccggccc
     5761 gcggggattc accctcgccc actacgcgcg cgtgctgggg gacccgccgc tgcagaaggc
     5821 gctctggaac acggtggtgc tcgccttctg gtccggcgtc gtcgccctcg cgatcggcgc
     5881 gccgatggcc tggctctccg cccgcgccga cctgcccggg cgccggatca tccagagcct
     5941 gatcatggcg tcgttcgtga cgccgccctt cctgggcgcc ttcgcgtggg tgatgctcgc
     6001 cggccccaac gcgggctatc tcaaccagct ctaccgcggc ctcaccggcg cggaggcgcc
     6061 gctgctcaac atcttctcga tgccgggctt gatcttcgtg gtggcgatct acacgttccc
     6121 gtacgtgtac atcatgatcg ccaacacgct cggcctgatc gcctccgatc tcgaggacgc
     6181 ggcggccatc ttgggcgcgg gccgcctgca ggtggcgcgc acgatcacgc tgccgatggt
     6241 gctgcccgcc atcctgagcg gcttcatcct ggcggtgctg caggcgctgg ccctcttcgg
     6301 ctcgcccgcc atcctggcgc tgcccgccgg cttccacacc gtcaccaccc agatgtggtc
     6361 gttcttccag tacccgccgc gcgccgagct ggccgccgcg ttctcgatcc cgctcctgct
     6421 cgccaccgcg ctcctgctgc tggtccagaa gaagctgctc gggcggcgcg gctacgcggc
     6481 ggtgggcggc aagggcgggc agcgacggac catccggctc ggcccctggc gctatccggc
     6541 gctcgcgggc tgcctggccg tgctggcctg cgcggtcttc ctgccctacg gcatcctgct
     6601 caaggccgcg ttcgcccgcg cctgggccca gccgctcacc cgcgggaact tcacgctcga
     6661 gaactggtcg ttcacctttc tccacagcgc gacccagtcc gcgatcgtca acacgctgga
     6721 gctgggcgtg ctcaccgcct gcgtgggcgc gagtctggcc accctcctcg cctacgtcac
     6781 caaccggcgg ctcgtgatgg gccaccaggt gctgggcttt ctcgcggtcg ccccggtggt
     6841 catcccggga gtggtgctgg cggtggccct cttcgtggcc tacacgcggc cgccgttcct
     6901 gctctacggc acgctgtgga tcctcttcat cgcctacctg accaaggaga tgccggtcgg
     6961 ctactcgcag gccgacgcca ccatgcgggg cattcacccg gagctggagg aggcgggccg
     7021 catcctgggc gcggggcggc tgcgggtgct gcgcgagatc accgcgccgc tcgcccggag
     7081 cgggatcatc gccacctggt gcttcgtctt catcggggtc atccgcgagc tgtcagcctc
     7141 catcatcctg ttcaccccga acaccaaggt catgtcggtg gtgatgttcg acctgaagga
     7201 agaaggccag ttcggcgcca tcgcggtcct gggcgtgttc atgctcgtga tgaccttcgc
     7261 cacggtggcg ctcatgcaga ccctgctcgg ccgcgacgtc ctgggcgcgc gatcctcgag
     7321 cgactcgggc tgatcgcatg ccggggatca ccatcaccgg tctcaccaaa cggttcggcg
     7381 acgtcgcggc ggtggccggg ctcgatctca cggtgcggcc cggcgagctg gtcgccctgc
     7441 tcggcccctc cggctgcggc aagaccacca cgctgcggct ggtcgcgggc ttcatggccc
     7501 cggacgcggg cgagatccgc gtgggcgagc gcgtgctctc gtccccgggc gcggtgatcc
     7561 ccccggagcg gcggcgcatg gcgatgatct tccagagcta cgcgctctgg ccgcacatga
     7621 cggtggcgca gaacgtggcc tacggcctcc gcttcacgaa gactccacgc gcccagcgcg
     7681 acggcaagat agacgagatc ctccgcgcgg tgcagctcgc gggctacggc gcgcgctacc
     7741 ccggcgagct gtcgggcggc cagcagcagc gggtggcggt ggcgcgcgcc ctggtggtgg
     7801 agcccgagat cctgctgctc gacgagccgc tctccaacct cgacgcgagc ctccgcgagg
     7861 agatgcgctt cgagatccgc cgcctgcacg agcgcttcgg catcaccacg ctctacgtca
     7921 cccacgatca ggcggaggcg atggtgatct cggaccgggc cgcggtgatc cgcaacggcc
     7981 gggtggagca gatcggcgcg ccgcaggagc tcttcgagcg gccgcgcacg cgcttcgtcg
     8041 ccgagttcat cggcaagacc aacctgatcg acacggtcgc cgacggcgcg ggcagcgtcg
     8101 cgcggggccg cctgcggctg cgcgtggccg ccgacggcct cacccccggg acgccggcgg
     8161 tggtgtcgat ccggccgcac ctcatcgcac tcgggccgcg cgccgatgcc aggatgccgg
     8221 ccgcgggcgc gaacgccctc gggggcaccg tgctgcgggc gagctacctc ggcgacacgg
     8281 tggactacca ggtcgcgctg gaggagagcg acgtcgtcct gcgggtcacc gggcccgccc
     8341 cggcgcgcgc ccgcgcgggc gacccggtct cggtgacggt cccggccgaa tcctgcgtcc
     8401 tgctccccgc ggaaagctga ctccttctac aacccgcccc agacctcgtc ggccagctcg
     8461 acgcagagcg cgagctttgc cttctggtgc tcgaccgcga cctcgttgcc ctcgatcgtc
     8521 gaggagaagc cacactgcgg gctcacgcag agctggtcga gcgcgatgta cctcgacgcc
     8581 tcgtccaggc gccgcttgag gtcggccctc cgctcgagca cgcccacctt ggtggtcacg
     8641 aggcccagca cgacccggat gtgcttcggg gcatggcgca ggggcgagaa gtcgccggag
     8701 cgcgcgtcgt cgaactcgag gaagaagccg tcgtagggca tctggttgaa gatggcctcc
     8761 gccactttct cgtagccacc ctgggcgcgc cagctcgagc ggaagttgcc ccggcagaga
     8821 tgcgtgcaga ccaccaggtc cttgggccgg tcgcgcaggg tggcctggat gagcttcacc
     8881 gacagggcca gcacctcatc gggatcctcg ccgcgggccc gaccctgcgc gcgcatgtcc
     8941 tcgtcgcaga gataggccag gctcacgtcg tcgagctgga gataccggca gcccgccgcg
     9001 tagagatcgg cgatctcggc gcggtaggcg gcggcggtgt cctccacgaa gccgtcgagg
     9061 tccgggtaga ccgcccggtc gatgttggcg cgccccacct gggagtagag catgcagggc
     9121 gacgggatcg tgagcttggc ggtgcgcgag gtggtcgatc ggaggaaccg gaacgcgtcc
     9181 acgaagatcg gcgcggggcg ccccacgcgc gaggcgatgc gcaggtcggg ccgcgtgaag
     9241 ttcacgtcgc ccgcgcggcc gtggaaggtc accgggagct gcttgccctc gctcgcgagc
     9301 ccgccgaggc gcgtgaggaa gtccatgtgc caggacttgc gccgcatctc gccgtcggtg
     9361 acgcccaaga gcccggcttc ctcctgaaga cgtaccccct cgcggatcgc cgcgtcctcg
     9421 gcggcggcca gctccgccgc gctcgcccgg cccgcttccc gctcgagccg cagcgcctta
     9481 agcgcgggcg gccgcaggag gctgcccacg tggtcggcgc ggaacggcgc gcggggcggc
     9541 gccatggcta gtagagcccc cgcccggccg cgaagacccg ggtgaggccg agcaacgcgt
     9601 ccgtgctggg cgtgggcacg ttcacgcgcc ggccgatctc gctcaccgcg gtgaggagcc
     9661 cgtcgatctc gagcggccgg cccgcctcca cgtcctgcag catggaggtc ttgaacgcgc
     9721 ccagcttgcg cgtcaccgcg ttccggtcct cgccgctctg gtgaatgggg cagccgatcc
     9781 ggtcgccgat cgccgccgcc tccgccatga tcgcgagcgt gaagcgctcg agcagcggat
     9841 cgtcgaggag acggtcgcag gtggccccgg tcagggccga catcgggttc atggtcatgt
     9901 tgccccacaa cttgtaccag atgtccgcct ggatgcggcg gctgaccgtg gtctcgaagc
     9961 cggccgcggc cagccgatcg gccagcgcgc tcacccggcc cgacaccccg ccgccgggct
    10021 cgccgatgat caacccccgg ccgaagccgt gcttcaccag acccggctcc tgcaccgagc
    10081 aggtggcgtg caccacgcag ccgatcacgt gccgcaccgg gatcgcggcc gcgacgcggc
    10141 cgtccgggtc caccgcccgc accgcggccc cctcgtgcgg cccaccgagg ccgtaaaaga
    10201 accaccacgg cactccgttc atcgcggtga gcgccacggt gtccgggccc agcagcggcc
    10261 ggatcgtcga ggcgaggccc ggcagcgacg gccccttcac cgcgagcacg accaaatcct
    10321 gaggcccgag cgcggccggc tcctccgcca cccgcaccgg cgcggtgagc accacgccgc
    10381 cctcctcgaa ccggaagccg tgggcccgca gcaccgccgc ggtcgtcccc cgcgcgacgg
    10441 ccgagacgtc ggagccgctc cgggccaggt acgtcccgag gaacgtgcca atggcccccg
    10501 cgccgacgat ggcgacggtc ttcattcgat cacgcggtcg gcccgcacga gcagcgactg
    10561 ggggacggtc aggcccaggc tcgcggcggt ccggcggttc acgctcaatt cgaagcgggc
    10621 cgggagctcg aggggaatgt ctcccgggcg cgtaccgcgc agcaccttca ccatcacctc
    10681 gcccgcctgc cggaacatgg ccggaacgtt gggcccgtag gagatcaacc ccccgtggtc
    10741 ggcaaactcc gggaacggca tgatcgtcgg catccggtgt cgcgccgccc cttccaccac
    10801 ctgcgggcgc gccgagtaga acaccgggga ggacagcacc atcagggcgt cggcccgctt
    10861 ctcggccgcc gagcggaacg cgggcgcgaa gtcctcggcg gtcagcgcct ccagggcaaa
    10921 gatctcgacc cgcagggctc gggcggccgc ctcggcgccc ttcagcaggt tcgacggcgt
    10981 ggagggatcc cagaccgccg cgatccgcga gagcttcggg accagctctc tcaggagctg
    11041 gatccacttg ccgctcagct cgggaaagtc gaggaacacg cccgtgacgt tcttcccggg
    11101 gcggcccagg ctggtcgcga atcccgccgc taccgggtcc gactccaggt cgaccgccac
    11161 gatcgggatg gacctggtcg tccctgccgc ggcccgcacc gccgcgggcc cgacggccaa
    11221 gatgccatcc atcccgaggc ggaccagctc gccggccagg accgccatgt cttcggtccg
    11281 gcccgcccgc acgcggaagt cgatcacgag attcgtgcct tccgtgaatc cgctgtcccg
    11341 caggggctgg aggaattgct cggcgttctg ccggaagatc ggggcatctg gcgccgcccg
    11401 cagcaccccg acccggaccg gcttcggcgc cctctgctgc gcccgggcgg agagcggtgc
    11461 ggcgaggatc ccgaacgcga tcccggccac gaaggccctg cggtccatgc gccgccgtca
    11521 ctccttgagg cgcaccacgt tgaaggcctt caccggccgc tgaaagccct tcagagtgag
    11581 ctcgccgaca ccctcgactt ccgcgatgtc ttccacgccg cccgccaccc gctgcgagag
    11641 caggatctgc ccgggcttgg cctcgccgca gagccgcgag gcgagattgg tcacggtgcc
    11701 gatggccccg tagtcgaggc ggccctcgaa gcccaccgcg ccgatggtgg cgtaaccctg
    11761 cgcgataccc accccgaaat cgagatcgaa tccgcgcttg cgccacgcct cggtcaggcc
    11821 ggcgatgcgc tcccgcatgg tcgcagccat gcggacggcc cgcaccgccg ggtcggagac
    11881 ctccaccggg tcgttgaaga agatcatcat gccgtcgccc gcgaagcgct cgagcgtgcc
    11941 ctccgcggcc aggatcagcc ggcccatctc cccgtggtac tcgcgcagca cggtcatgac
    12001 ctcttccggc tcggcggtct ccgcgaacgc ggtgaagccg cgcagatcga ggaagaccac
    12061 cgtgatctcg cggcggtggc tcttgagcgg atcctcggtg tcgccggcca cgatcagctc
    12121 ggcgagctgg ggcgagaaga agcggcgcag gcgccccagg cgctcgagct gggccacctg
    12181 gtcccgcacg cgctgctcga gcgtccggtt cagctcctcg agctcccggt gcagcgcgcg
    12241 gatgcgcagc agcgagcgca cgcgcgccag cagctcgggc tggttgatcg gcttgctcag
    12301 gaaatcgtcg gcgcccacct cgatgccctt gacccgctcc gtggtcggat cgagcgcggt
    12361 caccatcacg atcggcaaga gccgcgtggc ctcgtcggcc cgcagccgcc ggcagacctc
    12421 gtagccgctc atctccggca tcaccacgtc caggaggacg agatccggct gctcggcccg
    12481 cacccgctcg agcgcggccg cgcccgacga cgcggtggtc accgcgtagc cgtgcccggc
    12541 cagcacgccc tcgagcagct tgacgttcat cggcgtgtcg tccaccacca ggatgcgggc
    12601 cggctcgctc accgcgggcc ctcacccttc gcgggccgat ccagcatcga gcgcaccgcg
    12661 gccacgaact gggtcacgtc gatgggcttg gcctggtagc cgtcgaagcc ggccgccatg
    12721 atcttctgcc ggtcctgggt catggccgac gcggtcaccg cgatcaccgg gatcgctcgg
    12781 gtcctcgcgt cggatcgcag aacctggagc gcctcgatcc cgttcatgcg cggaagctgg
    12841 atgtccatga ggaccagggc gggcggcgtc gcctgcgcca gcgcgatccc gtcctccgcg
    12901 ttgtcggcct ccgcggtgcg gtacccgtgg aactggagga tgtcgcgctc cagcttccgg
    12961 ttcttctcgt tgtcctcgac gatcaggatc
//
LOCUS       DASUFA010000036        18492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1378330, whole genome shotgun sequence.
ACCESSION   DASUFA010000036 DASUFA010000000
VERSION     DASUFA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 18492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18492
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378330"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..414
                     /locus_tag="VFX87_01275"
     CDS             31..414
                     /locus_tag="VFX87_01275"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX4993562.1"
                     /translation="MRLAVLVVLGMIGTCLWGLPDAHAHAIILESAPRHEESLPSPKR
                     LVLRFNSRLEKPLCSVQLVGPRQKTIALLRQDSDTPADTLAYTLPPLDPGAYHARWKV
                     MAADGHVTEGVVLFTVTAPEPAGKR"
     gene            428..817
                     /locus_tag="VFX87_01280"
     CDS             428..817
                     /locus_tag="VFX87_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX4993563.1"
                     /translation="MSRARRLRGAALAGALLAGLLGPMPRAGAHASLVRSAPTHRAVL
                     GQMPERVQLWFNERIEPAYSTVSVWNEAGAQVDARDVAVGPDDPRLLSVTVEARQSGL
                     YTVKYRVLSVDGHIVDSRFTFTVKASR"
     gene            831..1388
                     /locus_tag="VFX87_01285"
     CDS             831..1388
                     /locus_tag="VFX87_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993564.1"
                     /translation="MTRLDNRIRSFLVVTVAATVVGLGAAAGWEAVRAQGHSHSQTQS
                     HAADPGGWYKRLSPDARAAAIERQLRGFETTMAEVAYRYTEMYFGGIDGNWEYAAHMG
                     HELGGAVSVGLERRPQYRKSADALFLKGPYLEVMDAIKAKDLALFKQRIETLRAACTA
                     CHAAEAHPFIKIGVPTVRRNPVVND"
     gene            complement(1419..2774)
                     /locus_tag="VFX87_01290"
     CDS             complement(1419..2774)
                     /locus_tag="VFX87_01290"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX4993565.1"
                     /translation="MTLIASWLVRWIGFVALAGLVGGFVVDLFILPAGAPETAVTRRR
                     LRSLRVGCTTLLLLAAGGDLWLRAGTMSGGGPGTAVQAVPSILTRTHYGSVWIVRGAG
                     LLALLPLSRASSRGFRGLGALVALGVALTVSLTGHAGDWGDVSPTAGIDWIHVVAATT
                     WTGGLFVLAALMLRDGPRWPIALLADAMRRFSGLAAWCLLAVVLSGAYASWVQLPSVA
                     ALWQTVYGRVLAAKLLLVLSLVWWGAVNRYTVLPGLGAGRASGVLERLFRIGRLALTG
                     SSRVPHPALSSRLAAYVTREAALAMLVFGCTAVLGESTPARHAGHLEHRSAPEEPVRI
                     TMADLHASGGVPRGWSFTPPAGDVARGRQVFVRSGCFACHAVAGESFPPASGVGPDLT
                     EAGEHHPAGYLLESVINPNVVIVQGPGHTGPDGLSIMPSYADRLAVQELLDLVAYLRA
                     L"
     gene            complement(2771..3118)
                     /locus_tag="VFX87_01295"
     CDS             complement(2771..3118)
                     /locus_tag="VFX87_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX4993566.1"
                     /translation="MRALWAVAALLVTAAPAWGHAFPDHSEPRVGHTVAAAPVVRIWF
                     DGAIEPVFSTLRVEDADKRRVDRNDARVSAGDGSLLEVSLPPLAPGRYQVFWSVVARD
                     GHRTEGSFAFRIK"
     gene            complement(3121..4059)
                     /locus_tag="VFX87_01300"
     CDS             complement(3121..4059)
                     /locus_tag="VFX87_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993567.1"
                     /translation="MAAAGHASAHGLIGQRFFPATLTIDDPFVADELSLPTVFHIRNR
                     GSEESPPTLQTDLSGEVSKRLSPNLGVSLGGTYTLLDPLPGKSVSGFDNMEVSLKYVF
                     WKSAAHETLLSAGVSWDVGGTGSKKIGAESFDTVTPQLFFGKGFGDLPNAVEGLRPLA
                     LTGALGLDIPTRRFNQTISVNDDGEVEVERELNAKTMQWGFSLQYSLQYLQSFVRDVG
                     LPTPFNRMIPIVEIAMQTPIEGPHAGRTTGTINPGVIWFGRYFQLGIEAVIPVNTMTG
                     KNVGVLAQAHFYLDDIAPRIFTWTPFHGVLGPTRPQ"
     gene            complement(4197..4634)
                     /locus_tag="VFX87_01305"
     CDS             complement(4197..4634)
                     /locus_tag="VFX87_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993568.1"
                     /translation="MADPGVHLLDTHAEAAHAVGHFALLASSLPTGGLLWAGLAFIAG
                     IGLLRLAAHRSRGRVIALGLSLALGVFTLESAVHSVHHLADPETAANCPVLSGSQHLS
                     WGEAQAAAADAPPPCVAPAPPLRAEDASPSLIYRPHQGRAPPA"
     gene            complement(4847..5401)
                     /locus_tag="VFX87_01310"
     CDS             complement(4847..5401)
                     /locus_tag="VFX87_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX4993569.1"
                     /translation="MKRTIAFVLLSALAVLPAVALAKSEPMVGGQAMYPSKDIVDNAV
                     NSADHTTLVAAVKAAGLVDTLKSQGPFTVFAPTNAAFSALPAGTVDTLLKPENKAGLT
                     KVLTYHVVPGRMDAAALQKAIKAGNGTATLKTASGGMLWAMMNGDRNIVLKDEKGAVA
                     SISTYDVYQSNGVIHVIDRVVLPN"
     gene            complement(5506..6003)
                     /locus_tag="VFX87_01315"
     CDS             complement(5506..6003)
                     /locus_tag="VFX87_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX4993570.1"
                     /translation="MAAENSFDVACKIDMQEVTNAIDQARRDIATRYDLKGAKNEIEI
                     EKMDITISSTDDMKVKAVLDILQSKLHRRGIDLKALTINDPVPAGGGLVRQKITLQDG
                     VPMEKAKEIVRLIKDSKIRVQASIQEKQVRVAGKNRDDLQAVIALLRGKDLGIALQFT
                     NYRST"
     gene            complement(6018..6698)
                     /locus_tag="VFX87_01320"
     CDS             complement(6018..6698)
                     /locus_tag="VFX87_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX4993571.1"
                     /translation="MKRGARQHILPHLCLLVLLLLAPVSADAQTLDEVVNGVESTYGK
                     IVDLRAEFTQTARNKSLGQDIKAEGTVLLKKGGKMRWDYKSPAPQQIVSDGTSLWVYT
                     PELNQVNKGNAPKALAGPAGSFLSGLGKLREEFTVRFLNPASPRDGAGRPVLDLTPKQ
                     PTPLLTRLVITVDPKDYIVRQAVLYDQFQNTVTMTFNRVAINPGISDTLFVFTPPKDA
                     VVVPLDPR"
     gene            complement(6734..8992)
                     /locus_tag="VFX87_01325"
     CDS             complement(6734..8992)
                     /locus_tag="VFX87_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX4993572.1"
                     /translation="MAEKGTIEKILPEKAPGANGHGPRETSRDGAPRRRGPRPGKAEP
                     GRWVREAKGILALALAGFGFVALYAYDPMVHPLDQSSPVGPVGAWLGWGSFQAFGYAG
                     YLFPVLLALYGVSAFVRPRVAHGWQAGVGLLILLVSATGILARASDTLAEYHLHKGGV
                     VGWAVSEGLVVSIGTVGTWIILLALIPVGVLFVTRIPYGVLSRTLRARFGRLRRPESK
                     KALRASPASADLPLVAAVPPGGWPADQPAAPPPFVVKEQPKVKSGLSDKGLAWQETFD
                     FGKGGAEAFKLPAVGLLAAPPASSLSRTREELEGNAQTLKKKLHDFGVDGHIVQASPG
                     PVITSYEFEPAAGVKVSQVVNLADDLALAMKVAAVRIIGPIPGRGTVAVEVPNPETAT
                     VYLREILVSPEFVTTKGKLPLALGKDTTGNPVVADLAAMPHLLIAGSTGSGKSVGLNT
                     MICSLLYKATPADVRFLLIDPKRLELGIYEKIPHLLAPVVTDAKEASARLKWIVGKMD
                     ARYKQLQAKAVRNIEGYNKEVPPEERLPYWVVVVDELADLMMVSAGEVQNSLIRLAQI
                     ARAVGIHLIIATQRPSVDVVTGLIKANFPTRIAFQVASKVDSRTVLDGNGAEQLLGRG
                     DMIFVPPGASRQMRVHGSWVSDKEVKAMCEFLRTQGSAVYETVELPADEAGPGGNGED
                     RDDIYWDAVNLVIAQRAASISFLQRRLGLGYPKAARFIDMMEQDRIIGPGHGAKPREI
                     LVGPEYLQKRGK"
     gene            complement(8992..10695)
                     /locus_tag="VFX87_01330"
     CDS             complement(8992..10695)
                     /locus_tag="VFX87_01330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX4993573.1"
                     /translation="MNTTYRPDPVPIDAPGEPAVRLIPLGGLGEIGLNMMLIECGPDI
                     LAVDCGLLFPDDEMPGVDFAIPDFAYLRERRDAFRGVVLTHGHEDHIGALSYLLREFP
                     VPVYGTPLTLAVAKHRLTENGMLEQSDLRAYAPGERIVIGGFTVEPIRVTHSIADGIG
                     LAIETPLGTVVHTGDFKLDAHPVDHQQPDYSRFAALGERGVLVLCSDSTNVGRPGHTG
                     SETLVGQALAGRFAEAPGRIIVATFASHIHRIQQVLDLAVHCHRNVALLGKSMMTNVA
                     VAQELGYLTVPEGLIVPLEELAELPAQRQVIVSTGSQGEPNSALSLIAAGDHKYVHAG
                     PGDVVIFSSRVIPGNERVIGRVINSLLRRGAEVLWEDVAFVHVSGHASQEDLKQMLAL
                     TRPRYFVPVHGEFRHLLQHARLAEGVGIPKDRIFLIEDGLGLEVTASGTRVLGGYPAG
                     PVLVDGKGVGDVGSVVLRDRQLLAEAGMLVIALTIDRTTGLIVAGPEIVSRGFVYMKE
                     ADALMDEVKTAVRDALAGRQEPEVFDRELVGAMVRSAARRFINQRFQRKPVVLPVILE
                     V"
     gene            complement(10747..11610)
                     /gene="rsmA"
                     /locus_tag="VFX87_01335"
     CDS             complement(10747..11610)
                     /gene="rsmA"
                     /locus_tag="VFX87_01335"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX4993574.1"
                     /translation="MSPRRNARAAVPRAPVYRSATRGRTRALGQHFLRDRSVVDRIVD
                     LVAPTARDLVVEIGPGRGALTETLAARAGRLLTLEIDAELVVTLQARFAASPHVEVRQ
                     ADARQFDYRTLRALLPDPGGRVLVVGNLPYSVGKPILAALVEAVPAIDEMALMLQKEV
                     AERVAAEPGGKTYGALSVLTQVSVAARLAFSVPPGAFSPPPQVDSAVLRLVAHRTPPV
                     PVADPARFAAVVRAAFGQRRKSLANALAAGLGLGVERARRLCEAAAIDPGRRAETLSL
                     AEFARLAATAG"
     gene            complement(11607..12239)
                     /gene="coaE"
                     /locus_tag="VFX87_01340"
     CDS             complement(11607..12239)
                     /gene="coaE"
                     /locus_tag="VFX87_01340"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380024.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX4993575.1"
                     /translation="MKRFLLVGLTGGIATGKSTVTAMLASPSVRVVDADALAREVVEP
                     GTPAHAQIVADFGKEVLQPDGRLDRARLGEIVFPDPDKRKRLEAITHPAIRVRFEGIM
                     ADLERQGFDGLLIWDAALLVESGGHNKMDKVVVVTTDPATQLRRLMARDGSTEEAARA
                     RTASQMPLAVKERAADYVIDNSGTREETAARVREVHRALLEDLRRLQARQ"
     gene            complement(12236..14800)
                     /gene="polA"
                     /locus_tag="VFX87_01345"
     CDS             complement(12236..14800)
                     /gene="polA"
                     /locus_tag="VFX87_01345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX4993576.1"
                     /translation="MTERLFILDGPGFLFRAYHAIPFLSTSKGVPSHAVFGMSTMLWK
                     LLREDNPEYFAVAWDPPGPTFREEKFAAYKETRAPTPDDLRTQIPLVKTLFEALRLPL
                     LEVPGFEADDVLGTVVDRTRDLPIDLVLVTSDKDMLQLVSPRVRVFSTTGRGGDRVIF
                     DEAAVKAKWGVEPAQIPDILALMGDSIDNIPGVPGVGEKTAAKLIGQFGGVARLYENL
                     SLVPGKLRETLAANRKQALLSRELATVSTRVPITVDLEAFRRREPDWERLRALWTELE
                     FHSLLRQLPAEPAPEATGGDVPTLADADALARYLARVPAGEPLAVDVVSAGGPPEPVV
                     ATIGLYHPVAGQAAVECGGPEMASALLAALGGRTLVGHDIKPLIEWWLAHGGAPPPGE
                     DTAVAAYLLNPARTNYKLEEVCAELLGQGPGIARPGTRARWIWDLWAMETRVLQEVGL
                     HVLYQDVERPLIGVLARMERHGIRVDPVRLGEFSRELEVHLERTTREIFTLAGEEFNI
                     GSPKQLAHILFEKLKLPPVRRTKTGYSTDADVLEQLAIGHELPARIVEHRTLAKLKST
                     YADSLPTLIHPATGRIHPSFNQLVAATGRLSSSNPNVQNIPVRTELGRRIRAAFVPDP
                     GWRFLAADYSQIELRILAHVSGEESLIEAFRRGEDIHARTASEVFGIALEAVTPEQRD
                     IAKTTNFAVIYGVTAFGLSRGLDMSPKQAQEFLDRFFARHPKVKAYLARTVAEGRERG
                     FVTTLLGRRRYLPELRSGNPNLRGFGERMATNAPIQGTAADLIKIAMVRMARELDAHR
                     LESRMLLQVHDELLFEVPPAELPRLQALATQVMESAMSFDVPLKVDVKIGDDWAAV"
     gene            14898..16190
                     /gene="murA"
                     /locus_tag="VFX87_01350"
     CDS             14898..16190
                     /gene="murA"
                     /locus_tag="VFX87_01350"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4993577.1"
                     /translation="MTGDRFLVEGGHPLRGTVTPAGNKNEALPVLAACLLVAGEVVVD
                     NVPRIRDVTALLEGLRHLGVRVEVDDHGTARLDASTLKGRDPDPAVMSQLRGSVLLAP
                     GLLARTGQAVLPPPGGDRIGRRRLDTHLLALSELGARVASGPTLHLTLEGRFRGTEVF
                     LDEMSVTGTENTVMAAVLAEGETVILNAASEPHVQGLCRFLNTLGARISGIGTNQLRI
                     QGVRELGPGRYRLGPDHIEIGSFVALAAMTGGDLRIRDVIPDDLRMIRLAYRRLGVET
                     SLDGTTLVVPGPQELTVAADADGAISKVDDAPWPGFPADLTSLALVLATQSRGTVLIH
                     EKLFESRLFFVDSLIAMGARIILCDPHRAVVVGPTELRAAPMVSPDIRAGMALIGAAL
                     CARGTSSIMNAHQVDRGYERIDERLRSLGARITREPGA"
     gene            complement(16271..17461)
                     /locus_tag="VFX87_01355"
     CDS             complement(16271..17461)
                     /locus_tag="VFX87_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX4993578.1"
                     /translation="MVTRPSVAIVGGGIGGLFAANALLARGLRVAVYEQATAIGEIGA
                     GVYVTPNAVRHLDSIGFGGAVERWGARVGPGSAYFRHDGAPIAPVQVTDAAGWNACFG
                     MHRADYIEFLAAHLPAGIIRTGHRAVGFEQTAEVGRVKFANGAVAEADVVIGADGIHS
                     ELRPHVFPPSKPLFHGTISYRGLVPRERLPAWPMDRWQMWAGPSKHFLVFPVRHGEMV
                     NYVGFVPTDEQMKESWSAPGDPEVLRREFCGWDPRIGQVLKQVDRTFRWALYDREPLP
                     TWTRGRLSLLGDAAHPMLPHLGQGANQAIEDGIALATILTRADTASAPAGLLAYERLR
                     RERVAEVQLGARRHGLRVDSMVDDLRKRDADLLAHAEFRKQLYSYDVVPHAEAAAKAA
                     GLSL"
     gene            complement(17527..17889)
                     /locus_tag="VFX87_01360"
     CDS             complement(17527..17889)
                     /locus_tag="VFX87_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993579.1"
                     /translation="MNLWDGIIRATPHPQELRGLRDRVGRGAHGEPGKLFRRSVGFSE
                     PLDGGSRLVSFLKQFGHFFAMILSVAAGLAFFAEWSEPGQGMAKVGVDDRGRTGPKPG
                     RPLPEGVHVAYPRGMAEF"
     gene            17888..>18492
                     /locus_tag="VFX87_01365"
     CDS             17888..>18492
                     /locus_tag="VFX87_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX4993580.1"
                     /translation="MSRGRGLLVLLVLLLVVAGILIYRLPAATPVREMLRSVKDTSQD
                     AATTSKVKTALLLSKHVSAFDIKATTTRGVVSVTGEVPSEETRRLAGAIAQDTSGVAH
                     VQNDLLVNPGAGRNSAMANLGDRIADLEVKTIVIDRLARAPGLKDKRLTVQVARQVVT
                     LEGTVDSPAQKRAAEDIAIQAPGVQSLVSQITVAAGQAGPE"
BASE COUNT         2768 a         6511 c         6563 g         2650 t
ORIGIN      
        1 cgatatcgcc ttcgcgcccg gcgtggcccg atgaggctcg cggtcctggt ggtcctgggt
       61 atgatcggga catgcctgtg ggggctcccc gacgcgcacg cgcacgccat catcctcgag
      121 tccgccccgc gccacgagga atcgctgccg agcccgaagc gactcgtcct gcgcttcaac
      181 agccgcctcg agaagccgct ctgctcggtg cagctcgtcg ggccgcgcca gaagaccatc
      241 gcgctgctgc gtcaggactc ggacaccccg gccgacaccc tcgcgtacac cctgccgccc
      301 ctggatccgg gagcctacca cgcgcggtgg aaggtgatgg ccgccgacgg ccacgtgacg
      361 gagggcgtcg tgctcttcac cgtgaccgcg ccggagccgg ccggaaagcg gtgaaggcgc
      421 ccgccccgtg agccgcgcgc ggcggttgcg cggtgcggcc cttgcgggcg cgctcctcgc
      481 cggtctcctc ggcccgatgc cacgcgcggg ggcccacgcc tccctggtgc gatcggcgcc
      541 cacgcatcgc gcggtgctgg gccagatgcc ggagcgcgtg cagctctggt tcaacgagcg
      601 gatcgagccc gcctacagca cggtgtcggt gtggaacgag gcaggcgcgc aggtggacgc
      661 gcgcgacgtg gcggtgggcc cggacgatcc gcgcctcctg tcggtcacgg tggaggcgcg
      721 gcagtccggc ctctacacgg tcaagtatcg cgtgctctcg gtggacggcc atatcgtcga
      781 cagtcgcttc acgttcacgg tgaaagcgtc acggtgaaag gactaccctc atgacacgac
      841 tcgataaccg gatccgctcg ttcctcgtgg tgacggtcgc cgccaccgtg gtcggcctgg
      901 gcgccgccgc gggctgggag gcggtgcgag cccagggcca cagccactcg cagacgcagt
      961 cccacgcggc ggacccgggc ggctggtaca agcgcctgtc gcccgacgcg cgggccgccg
     1021 ccatcgagcg gcagctccgc gggttcgaga ccacgatggc cgaggtcgca taccggtaca
     1081 ccgagatgta cttcggcggt atcgacggca actgggagta cgccgcccac atggggcacg
     1141 agctgggcgg cgcggtctcg gttggcctcg agcggcggcc gcagtaccgc aagagcgcgg
     1201 atgccctctt cctgaagggg ccgtacctgg aggtgatgga cgcgatcaag gccaaggatc
     1261 tcgcgctgtt caagcagcgg atcgagaccc tgcgcgcggc ctgcacggcc tgccacgcgg
     1321 cggaggcgca tccgttcatc aagatcgggg tgccgacggt gcggcggaac cccgtcgtca
     1381 acgactgacc gggcggcgga tcgcggctgg gggcggcgtc agagcgcacg gaggtaggcg
     1441 acgagatcca gcaactcctg gaccgccagt cgatcggcat aactcggcat gatcgacagg
     1501 ccgtccggcc ccgtgtggcc cgggccctgc acgatgacca cgttcggatt gatcaccgac
     1561 tccagcaggt agccggccgg gtgatgctcg cccgcttcgg tgagatcagg gccgacgccg
     1621 gaggccggcg ggaagctctc gccggccacc gcgtgacagg cgaagcaacc ggagcggacg
     1681 aagacctgcc gcccgcgcgc gacgtcgccc gccgggggcg tgaagctcca cccgcgcggt
     1741 acgccgccgc tcgcgtggag gtcagccatg gtgatccgga ccggctcctc cggcgccgag
     1801 cgatgctcga gatgccccgc atgccgggcg ggcgtggatt cgccgaggac ggcggtgcac
     1861 ccgaagacga gcatcgcgag ggcggcctcg cgcgtcacgt acgcggcaag tcgagacgac
     1921 agagccgggt gcggaactcg cgaagagccg gtcagcgcca gccgcccgat gcgaaatagc
     1981 cgctcgagga cacccgaggc acgtccggcg ccgaggcccg ggagcacggt atatcgattg
     2041 accgctcccc accagacgag gctcaggacc aggaggagct tcgcagccag cactcggccg
     2101 tagaccgtct gccagagcgc ggccacactc gggagctgca cccacgaggc gtacgcgccg
     2161 ctcaggacca ccgccagcag gcaccaggcc gcgagaccgg agaagcgacg catcgcatca
     2221 gccaggagcg cgatcggcca gcgcggaccg tcccgtagca tgagggcggc caggacgaag
     2281 aggccgccgg tccacgtggt cgcggcgaca acatgaatcc agtcgattcc ggccgtcggg
     2341 gacacatccc cccagtcgcc ggcgtggccg gtcaggctca ccgtgagggc gaccccgagg
     2401 gcgaccagcg cccccagacc ccgaaacccc cgcgatgacg ctcgtgagag tggcagcaat
     2461 gccagcaggc ctgccccgcg cacgatccac accgagccgt aatgcgtgcg cgtcaatatg
     2521 gagggtacgg cctgcaccgc cgtcccgggg ccgccaccgc tcatggtgcc ggctcgcagc
     2581 cacaggtccc cgcccgccgc gaggagaagg agcgtggtgc agccgacccg caggctccgg
     2641 agccggcggc gcgtgaccgc tgtctccgga gccccggcgg gcaagatgaa caggtccacg
     2701 acgaagcccc cgaccaggcc ggccagcgcg acgaacccga tccagcggac gagccacgac
     2761 gcgatgagcg tcacttgatc cggaatgcga agctgccttc cgtgcgatgc ccgtcgcgcg
     2821 ccacgacgct ccagaagacc tgataccggc cgggggccag cggcggcagg ctgacctcga
     2881 gcaggctccc gtcgcccgcg ctcacgcggg catcgttccg gtccacccgc cgcttgtcgg
     2941 cgtcctcgac acggagggtg ctgaagaccg gctcgatcgc gccatcgaac cagatgcgca
     3001 ccacgggcgc ggcggccacg gtgtggccaa cccggggctc ggaatgatcc gggaacgcgt
     3061 gcccccaggc cggagccgcg gtgaccagca gcgccgccac cgcccacagt gcgcgcacgg
     3121 ctactggggc cgggtcggcc cgagcacgcc gtggaacggc gtccacgtga agatcctcgg
     3181 cgcgatgtcg tccaggtaga agtgggcctg ggccaacacg ccgacgttct tgccggtcat
     3241 ggtgttgacg gggatgaccg cctcgatgcc gagctggaag tagcgcccga accagatcac
     3301 tccgggattg atcgtccccg tcgtgcgccc ggcgtgcggc ccctcgatcg gcgtctgcat
     3361 cgcgatctcc acgatcggga tcatgcggtt gaacggggtc gggagcccca cgtcccggac
     3421 gaacgactgg aggtactgca ggctgtactg cagcgagaac ccccactgca tggtcttggc
     3481 attcagctcg cgctccacct cgacctcgcc gtcgtcgttg accgagatgg tctggttgaa
     3541 acggcgcgtc gggatgtcga gaccgagggc gcccgtcagg gcgaggggcc ggagcccctc
     3601 caccgcattc ggcagatcgc cgaagccctt gccgaagaag agctgcggcg tgaccgtatc
     3661 gaaggactcc gccccgatct tcttgctgcc ggtccccccg acgtcccagc tcacgccggc
     3721 ggagagcagg gtctcgtgcg cggcgctctt ccagaagacg tacttgagcg agacctccat
     3781 gttgtcgaac cccgagaccg acttcccggg aagggggtcc agcaaggtgt aggtgccgcc
     3841 gagcgagacc cccaggttcg ggctcaggcg tttcgagacc tccccgctca gatccgtctg
     3901 gagtgtcggc gggctctcct cggacccgcg gttccggatg tgaaagacgg tcggcagcga
     3961 tagctcgtcg gccacgaacg ggtcatcgat ggtcagggtg gccgggaaga agcgttgacc
     4021 gatcagcccg tgagcggacg cgtgacccgc cgcggccatc accagcgccg tcgcccatag
     4081 caggacagca cagggtcttg atctcatgga ggtccccccg cgcgggcggg ccggccggac
     4141 aggctccacc tagcgctact tgttgtgagg tccgggaagc gatccgggga gcgaggtcag
     4201 gcgggaggag cccgtccctg gtggggacgg tagatcagcg acggcgaggc gtcttcggcc
     4261 cggaggggcg gcgccggagc cacgcaaggc ggtggagcat cggccgcggc cgcctgggcc
     4321 tcgccccacg agagatgctg ggagccggag agcacggggc aattcgcggc cgtttcgggg
     4381 tcggccaggt gatggaccga gtggacggcc gactcgaggg tgaagacgcc cagcgccagc
     4441 gagaggccga gagcgatcac gcggccgcgc gagcggtggg ccgccaggcg cagcaggccg
     4501 ataccggcga tgaatgcgag cccggcccac aacagaccgc cggtcggaag gctggacgcg
     4561 agaagcgcga agtggccgac cgcgtgagcc gcttccgcgt gggtgtccag gagatggact
     4621 cccgggtccg ccatcacggg gccagcggcc agcaagaagg acagcgcgcc gagcgcggcg
     4681 agggctatcg agcaccgggg acgtcgagcc atgacgaatt gcggctcact ctgcgcgcgg
     4741 gttccggagt tgtcaaggcc acggccggct ccgcgggagg aacgcatggc ggcacgggct
     4801 cttggggccc gtgccgccgg ggtgtggcgt gacggcgtgc gcgcggctag ttggggagca
     4861 ccacgcggtc gatcacgtgg atcacgccgt tggactggta gacgtcgtac gtcgagatgc
     4921 tggccaccgc gcccttctcg tccttgagca cgatgttgcg gtcaccgttc atcatggccc
     4981 acagcatgcc gccgctggcc gtcttgagcg tggcggtgcc gttgccggcc ttgatggctt
     5041 tctgcagcgc ggcggcgtcc atcctgccgg gcaccacgtg ataggtcagc accttcgtga
     5101 gacccgcctt gttctccggc ttgagcaggg tgtccaccgt gcccgcgggg agcgccgaga
     5161 acgcggcatt ggtgggggcg aagaccgtga acggcccctg gctcttgagg gtgtccacga
     5221 ggccggccgc cttgaccgcc gcgaccagcg tggtgtggtc cgccgagttc acggcgttgt
     5281 ccacgatgtc cttgctcggg tacatcgcct gcccacccac catcggctcg ctcttggcga
     5341 gcgccacggc gggcaggacg gccagagcgc tgagcagcac gaacgcgatt gtccgcttca
     5401 tatcgtccca tcctttccgg ctagaggttc cccgggcgga gggggccggc gggcgccccg
     5461 ctctccgggc tcaccctgac ttacggccgg ggggacgggg cggatctacg tggagcggta
     5521 attcgtgaac tggagcgcga tgcccagatc cttgcctcgg agcagcgcga tgaccgcctg
     5581 caggtcgtcg cggttcttgc ccgcgacccg cacctgcttc tcctggatcg acgcctggac
     5641 tcggatcttg gagtccttga tgaggcggac gatctccttg gccttctcca tcggcacgcc
     5701 gtcctgcagc gtgatcttct gacgcacgag gccgccgccc gcgggcaccg gatcgttgat
     5761 ggtcagcgcc ttgagatcga tgccgcggcg gtggagcttg gactgcagga tgtcgagcac
     5821 ggccttgacc ttcatgtcgt cggtcgagga gatcgtgatg tccatcttct cgatctcgat
     5881 ctcgttcttc gcgcccttca gatcgtagcg cgtcgcgatg tcgcggcggg cctggtcgat
     5941 ggcgttggtg acctcctgca tgtcgatctt gcaggcgacg tcgaagctgt tctcggcggc
     6001 catggcgagg tcctcggcta gcgcgggtcg agcggcacca ccaccgcgtc cttcggcggc
     6061 gtgaagacga agagcgtgtc gctgatgccc ggattgatgg cgacccgatt gaaggtcatg
     6121 gtgaccgtgt tctggaactg gtcgtagagc accgcctggc ggacgatgta gtccttggga
     6181 tccaccgtga tcaccaggcg cgtgaggagc ggggtcggct gcttgggcgt gagatcgagc
     6241 accggccggc cggcgccgtc gcgcgggctg gccgggttga ggaagcgcac cgtgaactcc
     6301 tcgcgcagct tgccgagccc ggagaggaag ctgcccgccg gtccggcgag ggccttgggc
     6361 gcgttgccct tgttcacctg gttcagctcg ggcgtataga cccagagcga ggtgccgtcc
     6421 gagacgatct gctgcggcgc cgggctcttg tagtcccacc gcatcttgcc gcccttcttg
     6481 agcagcacgg tgccctcggc cttgatgtcc tggccgaggc tcttgttgcg ggcggtctgg
     6541 gtgaactcgg cgcggagatc cacgatcttg ccgtaggtgg actcgacgcc gttgaccacc
     6601 tcgtcgaggg tctgagcgtc cgccgagacg ggcgcgagga ggaggagcac gagcagacag
     6661 agatgtggca ggatatgctg acgcgctcct cttttcatat tgctcgcctc gccttcggct
     6721 tcagctcgca cggctactta cccctcttct gcaggtactc gggaccgacg aggatctcgc
     6781 gcggcttggc gccgtggccg ggcccgatga tgcggtcctg ctccatcatg tcgatgaagc
     6841 gcgcggcctt gggatagccc aggccgaggc ggcgctgcag gaacgagatg gacgcggccc
     6901 gctgcgcgat caccaggttc accgcgtccc agtagatgtc gtcgcggtct tcaccgttgc
     6961 cgcccggacc ggcctcgtcg gcgggcagct ccaccgtctc gtacaccgcg gagccctgcg
     7021 tgcgcaggaa ctcgcacatg gccttcactt ccttgtcgga gacccaggag ccgtgtaccc
     7081 gcatctgccg gctcgcgccc ggcggcacga agatcatgtc gccgcggccc aggagctgct
     7141 cggccccgtt gccgtccagc acggtccgcg agtccacctt cgaggccacc tggaaggcga
     7201 tgcgcgtcgg gaagttggcc ttgatcaggc cggtgaccac gtccaccgac ggccgctggg
     7261 tggcgatgat caggtggatg cccaccgcgc gggcgatctg ggccagtcgg atcagcgagt
     7321 tctggacctc gccggccgag accatcatga ggtcggccag ctcatcgacc accaccaccc
     7381 agtagggcag ccgctcttcc ggcgggactt ccttgttgta gccctcgatg ttgcgcaccg
     7441 ccttggcctg gagctgcttg tagcgggcgt ccatcttgcc cacgatccat ttgagccgcg
     7501 cggaggcttc cttcgcgtcg gtgacgaccg gcgcgagcag gtgcgggatc ttctcgtaga
     7561 tgccgagctc gagccgcttc gggtcgatca gcaggaaccg gacgtccgcg ggcgtggcct
     7621 tgtagagcag cgagcagatc atggtgttga ggcccaccga cttgccgctg ccggtggacc
     7681 cggcgatgag caggtgcggc atggcggcca ggtccgcgac caccgggttg ccggtggtgt
     7741 ccttgccgag ggccagcggg agcttcccct tggtcgtgac gaactccggg ctcaccagga
     7801 tctcgcgcag gtacaccgtc gcggtctccg ggttcggcac ctcgaccgcc accgtgccgc
     7861 ggccggggat cggcccgatg atccgcaccg ccgccacctt catggccagg gccaggtcgt
     7921 cggccaggtt caccacctgg ctcaccttga cgcccgccgc cggctcgaac tcgtaggacg
     7981 tgatgaccgg gcccgggctc gcctgcacga tgtggccgtc caccccgaag tcgtggagct
     8041 tcttcttcag cgtctgcgcg ttgccctcca gctcctcgcg cgtgcgcgag agcgacgagg
     8101 ccggcggcgc cgcgagcagg ccgaccgcgg ggagcttgaa cgcctccgcg ccgcccttgc
     8161 cgaagtcgaa ggtctcctgc caggcgaggc ccttgtccga gagccccgac ttcaccttcg
     8221 gctgctcctt cacgacgaat ggcggcggcg cggccggctg atccgccggc cacccgcccg
     8281 gcggcaccgc ggcgaccagt ggcaggtccg ccgacgccgg gctcgcgcgc agcgccttct
     8341 tgctctccgg tcgccgcaga cgtccgaagc gcgcccgcag cgtgcgcgag agcacgccgt
     8401 acgggatgcg cgtgacgaag agcacgccga ccgggatcag cgcgagcagg atgatccagg
     8461 tgcccaccgt gccgatcgag accacgagcc cttcggagac cgcccagccc accacgccgc
     8521 ccttgtgcag gtggtactcg gccagcgtgt cggacgcgcg ggcgaggatg ccggtggcgc
     8581 tcaccagcag gatcaggagc ccgacgccgg cctgccagcc gtgggccacg cggggccgca
     8641 cgaaggcgga gacaccgtaa agcgcgagca gcaccgggaa gagatagccc gcgtagccga
     8701 aggcctggaa ggagccccag ccgagccagg ccccgaccgg gcccaccggg ctcgactgat
     8761 cgagcggatg caccatcggg tcgtaggcgt agagggccac gaagccgaag ccggccaggg
     8821 ccagggccag gatgcccttg gcctcgcgca cccagcggcc gggctcggcc ttccccggcc
     8881 gcggcccgcg gcgtcggggc gctccgtcgc gcgaggtctc tcgcgggccg tggccgttcg
     8941 cgcccggggc cttctcgggc aggatcttct cgatcgtccc cttctccgcc atcacacctc
     9001 caggatcacg ggcaatacca ccggcttgcg ctggaaccgc tggttgatga agcggcgcgc
     9061 cgcgctccgc accatcgcgc ccaccagctc gcggtcgaac acctccggct cctgccggcc
     9121 ggccagggcg tcccgcactg cggtcttgac ctcgtccatc agggcgtcgg cttccttcat
     9181 gtagacgaac ccgcgcgaga cgatctcggg tcccgccacg atcaagccgg tcgtgcggtc
     9241 gatggtgagc gcgatcacca gcatgcccgc ctcggccagg agctggcggt cgcggagcac
     9301 cacgctgccc acgtcgccca cgcccttgcc gtccaccagc accggcccgg ccgggtagcc
     9361 gccgagcacc cgggtaccgg acgcggtgac ctcgagtccg aggccgtcct cgatgaggaa
     9421 gatgcggtcc ttggggatgc ccaccccctc ggccagccgg gcgtgctgga gcaggtgccg
     9481 gaactcgccg tgcaccggca cgaagtagcg cgggcgcgtc agcgcgagca tctgcttgag
     9541 atcctcctgg ctcgcgtgcc ccgagacgtg cacgaaggcc acgtcctccc agagcacctc
     9601 cgcgccgcgg cgcaggagcg agttgatcac gcgaccgatc acgcgctcgt tgcccgggat
     9661 cacccgcgag gagaagatca ccacgtcgcc cggccccgcg tggacgtact tgtggtcgcc
     9721 cgccgcgatc agcgacagcg ccgagttggg ctcgccctgg ctgccggtgg agacgatcac
     9781 ctggcgctgg gcgggcagct cggccagctc ctcgagcggc acgatcagcc cctccggcac
     9841 cgtgaggtag cccagctcct gggccaccgc cacgttcgtc atcatgctct tgccgagcag
     9901 cgcgacattg cgatggcagt gcaccgccaa atcgagcacc tgctggatgc ggtggatgtg
     9961 ggaggcgaac gtggcgacga tgatgcggcc cggcgcctcc gcgaagcggc ccgccagcgc
    10021 ctggccgacg agcgtctcgg agcccgtgtg ccccggccgg cccacattgg tcgagtccga
    10081 gcagagcacg agcaccccgc gctcgcccag cgccgcgaag cgcgagtagt ccggctgctg
    10141 gtgatccacc gggtgcgcgt cgagcttgaa gtcgccggtg tggaccacgg tgccgagcgg
    10201 cgtctcgatg gcgaggccga tgccgtcggc gatcgagtgc gtcacgcgga tcggctccac
    10261 cgtgaagccg ccgatgacga tgcgctcgcc cggcgcgtag gcgcgcaggt cggactgctc
    10321 gagcatgccg ttctcggtga gccggtgctt ggccaccgcc agggtcagcg gcgtgccgta
    10381 caccggcacc gggaactccc gcaggaggta ggacagcgcg ccgatgtggt cctcgtggcc
    10441 gtgcgtgagc acgacgccgc ggaaggcgtc gcgccgctcg cggagatacg cgaagtccgg
    10501 gatggcaaaa tccacgccgg gcatctcgtc gtccgggaag agcaggccgc agtccaccgc
    10561 caggatgtcg gggccgcact cgatcagcat catgttgagc ccgatctcgc ccaggccgcc
    10621 caggggaatg agcctcaccg ccggctcgcc gggcgcatcg atgggaacgg gatctggtct
    10681 gtaagtggtg ttcattgcgg ccttttcatc ctaccaccgg gccctagccg ggcccaagga
    10741 acggggctag cctgccgtgg cggcgagccg ggcgaattcg gccagggaga gggtctcggc
    10801 gcgacggccg gggtcgatcg cggcggcctc gcacagccgg cgggcgcgct cgacccccag
    10861 gcccaggccg gccgccaggg cgttcgccag gctcttgcgg cgctggccga aggcggcgcg
    10921 caccaccgcg gcgaagcggg cgggatcggc caccggcacg ggcggcgtgc ggtgcgccac
    10981 gaggcgcagc accgccgagt ccacctgcgg cggcgggctg aaggccccgg gcggcaccga
    11041 gaaggccagg cgcgccgcga ccgagacctg ggtcagcacc gagagcgcgc cataggtctt
    11101 cccgccgggc tcggcggcca cgcgctccgc cacctccttc tggagcatga gcgccatctc
    11161 gtcgatggcc gggacggcct cgaccagggc cgctaggatc ggcttcccca cgctgtacgg
    11221 caggttgccg accaccagca cgcggccgcc gggatcgggc aggagggcgc gcagcgtccg
    11281 gtagtcgaac tggcgcgcgt ccgcctggcg cacctccacg tggggcgacg cggcgaagcg
    11341 ggcctggagc gtcacgacca gctccgcgtc gatctcgagc gtgaggagcc gtccggcgcg
    11401 ggcggccagc gtctcggtga gggccccgcg cccggggccg atctcgacga ccaggtcgcg
    11461 cgcggtcggc gccaccagat ccacgatgcg atccaccacc gagcggtccc gcaggaagtg
    11521 ctggccgagc gcgcgggtgc ggccgcgcgt ggcggaccgg tacaccgggg cgcgcgggac
    11581 cgccgcgcgc gcgttacggc gcgggctcac tgccgcgcct ggagccgccg caggtcctcg
    11641 agcagcgcgc ggtggacctc gcgcacgcgc gcggccgtct cctcgcgggt gcccgagttg
    11701 tcgatgacgt agtcggccgc gcgctccttc acggccagcg gcatctggct cgcggtgcgc
    11761 gcgcgcgcgg cctcctcggt cgagccgtcg cgcgccatca gccggcggag ctgggtggcc
    11821 gggtcggtgg tcaccaccac caccttgtcc atcttgttgt gcccgccgga ctccaccagc
    11881 agggcggcgt cccagatcag gaggccgtcg aagccctggc gctcgagatc cgccatgatc
    11941 ccctcgaagc ggacgcggat cgccgggtgg gtgatggcct cgagccgctt ccgcttgtcc
    12001 ggatccggga agacgatctc gcccagccgc gcgcggtcga gccggccgtc gggctgcagc
    12061 acctccttgc cgaagtcggc cacgatctgg gcgtgggccg gcgtgcccgg ctccaccacc
    12121 tcgcgggcca gcgcgtcggc gtccaccacc cgtacgctcg ggctcgccag catggcggtc
    12181 accgtgctct tgccggtggc gatgccgccg gtgaggccaa ccaggaggaa acgcttcata
    12241 ccgcggccca gtcgtccccg atcttcacgt ccaccttgag cggcacgtcg aacgacatcg
    12301 cggactccat cacctgcgtg gccagcgcct ggagacgcgg gagctccgcg ggcggcacct
    12361 cgaacagcag ctcgtcgtgc acctgcagca gcatgcggct ctccagccgg tgcgcgtcca
    12421 gctcccgggc catccggacc atcgcgatct tgatcaggtc cgcggcggtg ccctggatgg
    12481 gcgcgttggt ggccatgcgc tcgccgaagc cccggagatt cgggttgccg ctgcgcagct
    12541 ccggcaggta gcgccggcgc ccgagcagcg tggtgacgaa cccgcgctcg cgaccctccg
    12601 cgacggtgcg cgcgaggtag gccttcacct tcggatgccg cgcgaagaac cggtcgagga
    12661 actcctgcgc ctgcttgggg ctcatgtcga ggccgcgcga gaggccgaag gcggtgacgc
    12721 cgtagatcac cgcgaagttc gtggtcttgg cgatgtcccg ctgctccggg gtcaccgcct
    12781 ccagcgcgat cccgaagacc tccgaggcgg tgcgggcgtg gatgtcctcg ccgcgccgga
    12841 aggcctcgat caggctctcc tcgccggaga cgtgggcgag gatgcgcagc tcgatctgcg
    12901 agtagtccgc cgcgaggaac cgccagcccg gatcgggcac gaaggcggcg cggatgcgcc
    12961 ggcccagctc ggtgcgcacc gggatgttct ggacgttcgg gttgctcgac gataaccgcc
    13021 cggtcgcggc cacgagctgg ttgaacgagg ggtggatgcg cccggtcgcc ggatggatca
    13081 gggtgggcag cgagtccgcg taggtggact tgagcttggc gagcgtccgg tgctcgacga
    13141 tgcgcgcggg cagctcgtgg ccgatcgcga gctgttcgag cacgtccgca tcggtcgagt
    13201 agccggtctt cgtccgcctc accggcggca gcttgagctt ctcgaacagg atgtgggcga
    13261 gctgcttggg tgagccgatg ttgaactcct cgcccgcgag ggtgaagatc tcgcgcgtcg
    13321 tccgctcgag atgcacctcg agctcgcgcg agaactcgcc gaggcggacc gggtccaccc
    13381 ggatgccatg gcgctccatg cgcgcgagca cgccgatcag cggccgctcg acgtcctggt
    13441 agaggacgtg gagccccacc tcctgcagca cgcgcgtctc catcgcccag agatcccaga
    13501 tccaccgggc ccgggtgccg ggccgcgcga tgcccggccc ctggccgagc agctcggcgc
    13561 agacctcctc gagcttgtag ttggtgcgcg cggggttcag caggtaggcg gccaccgcgg
    13621 tgtcctcgcc cggaggcggc gcgccgccgt gcgccagcca ccactcgatc agcggcttga
    13681 tgtcgtgacc gaccagcgtg cgcccgccga gagccgcgag cagcgccgac gccatctccg
    13741 ggcccccgca ttcaaccgcc gcttgaccgg ccaccgggtg atacagaccg atcgtcgcga
    13801 caacgggctc cggcggccct cccgcgctca ccacgtccac cgcgagcggc tcgcccgcgg
    13861 gcacccgggc caggtaccgt gcgagcgcat ccgcgtcggc cagggtcggg acgtcgccgc
    13921 cggtggcctc gggcgcgggc tccgcgggaa gctggcgcag gaggctgtgg aactcgagct
    13981 cggtccagag cgcgcgcagc cgctcccagt cgggctcacg gcgccggaaa gcctccagat
    14041 ccaccgtgat cggcacccgc gtgctcacgg tcgcgagctc gcgggagagc agcgcctgct
    14101 tgcggttggc ggccagcgtc tcgcgcagct tccccggcac cagtgagagg ttctcgtaga
    14161 ggcgcgccac gccgccgaac tggccgatca gcttggccgc ggtcttctcg cccacgcccg
    14221 gcacgcccgg gatgttgtcg atggagtcgc ccatcagggc caggatgtcg gggatctgcg
    14281 cgggctccac tccccacttg gccttcaccg ccgcctcgtc gaagatcacg cggtccccgc
    14341 cccggccagt ggtcgagaac acgcgcaccc gcgggctcac gagctggagc atgtccttgt
    14401 cggaggtgac gaggacgaga tcgatcggga gatcgcgggt gcggtccacc acggtgccga
    14461 gcacgtcgtc ggcctcgaag cccggtacct ccaggagcgg caggcgcagg gcctcgaaga
    14521 gcgtcttcac gagcgggatc tgggtgcgga ggtcatcggg ggtcggcgcc cgggtctcct
    14581 tgtaggccgc gaacttctcc tcccggaagg tcgggccggg cgggtcccag gccaccgcga
    14641 agtactccgg attgtcctcg cgcaggagct tccacagcat cgtggacatg ccgaagaccg
    14701 cgtgactcgg cacgcccttc gaggtggaga ggaagggaat cgcgtgatag gcgcgaaaga
    14761 ggaagccggg gccatcgagg atgaacaagc gctcggtcat gcgggctcgg accggatcat
    14821 acaccggcct cccggcgacc gcctctcccg tgcctttaat gtccgcacgc tcggttagta
    14881 ttgacgagga ggctcgcgtg accggagatc gcttcctggt ggagggcggc catcccctgc
    14941 gggggacggt gacgccggcc ggcaacaaga acgaggccct gcccgtgctc gcggcctgcc
    15001 tgctcgtcgc cggggaggtc gtcgtggaca acgtgccccg catccgcgac gtgaccgcgc
    15061 tgctcgaagg gctccgccac ctgggcgtgc gggtggaggt ggacgaccac ggcaccgcgc
    15121 gcctggacgc gagcacgctc aagggccgcg accccgaccc cgcggtgatg tcgcagctcc
    15181 gcggctcggt gctgctggcg ccgggcctgc tcgcgcggac gggccaggcg gtgctgccgc
    15241 cgcccggcgg cgaccggatc ggccggcggc gcctcgacac ccacctgctc gcgctgagcg
    15301 agctgggcgc acgggtcgcg tccggcccca cgctccacct gacgctcgag ggccgcttcc
    15361 ggggcaccga ggtcttcctc gacgagatga gcgtgaccgg caccgagaat acggtgatgg
    15421 cggcggtgct cgccgagggc gagaccgtga tcctaaacgc ggccagcgag ccgcacgtcc
    15481 agggcctgtg ccggttcctc aacacactgg gcgcccggat cagcggcatc ggcaccaacc
    15541 agctccgcat ccagggcgtc cgcgagctcg ggcccggccg gtaccgcctg gggcccgatc
    15601 acatcgagat cggctccttc gtggcgctgg ccgcgatgac gggcggcgat ctccgcatcc
    15661 gcgacgtgat cccggacgat ctgcgcatga tccgcctggc ctaccggcgg ctgggcgtgg
    15721 agacctcgct cgacggcacc acgctggtcg tccccgggcc gcaggagctc accgtcgcgg
    15781 ccgacgcgga cggggcgatc tccaaggtgg acgacgcgcc gtggccgggc ttccccgccg
    15841 acctgacctc cctcgcgctc gtgctggcga cccagagtcg cgggaccgtc ctcatccacg
    15901 agaagctatt cgagagccgc ctcttcttcg tggatagcct catcgcgatg ggcgcccgga
    15961 tcatcctgtg cgatccgcac cgggcggtgg tggtcggccc caccgagctg cgggccgcgc
    16021 cgatggtctc ccccgacatc cgcgcgggca tggccctcat cggcgccgcg ctgtgcgccc
    16081 gcggcacgtc ctcgatcatg aacgcgcacc aggtggaccg cggctacgag cggatcgacg
    16141 agcgcctgcg ctcgctcggc gcgcgcatca cccgcgagcc gggcgcctga tccggccggc
    16201 tcacttcggc acgacgcggt cgtagagtgc ccgcacccac gcggtgcggc gacggtagtc
    16261 cacgactttc ttacaacgaa agtcccgctg ccttcgccgc agcctcggcg tgcggcacga
    16321 cgtcgtacga gtagagctgc ttgcggaatt cggcatgcgc gagaagatcg gcgtcgcgct
    16381 tgcgcaggtc gtcgaccatc gaatcgacgc gcagcccatg cctgcgggcg ccgagctgga
    16441 cctcggcgac gcgctcgcgg cgcagccgct cgtaggcgag caggccggcg ggcgcgctgg
    16501 ccgtgtcggc gcgggtgagg atggtggcga gcgcgatgcc gtcctcgatc gcctggttgg
    16561 cgccctggcc gaggtgcggc agcatcggat gcgccgcgtc cccgagcagg ctgagacggc
    16621 ccctggtcca ggtcggcagc ggctcgcggt cgtagagcgc ccagcggaac gtccggtcga
    16681 cctgtttcag cacctgcccg atgcgcggat cccacccgca aaactcacgg cgcagcacct
    16741 ccgggtcgcc tggtgccgac cacgactcct tcatctgctc gtcggtcggc acgaacccga
    16801 cgtagttgac catctcgccg tggcgcacgg ggaagacgag gaagtgcttg gacgggccgg
    16861 cccacatctg ccagcgatcc attggccagg ccggcaggcg ctcgcgcggc accaggccac
    16921 ggtacgagat cgtgccgtgg aagagcggct tcgagggcgg gaagacgtgg ggccgcagct
    16981 cggagtggat gccgtcggcg ccgatcacca cgtcggcctc ggcgacggcg ccgttggcga
    17041 acttcacgcg gccgacctcc gcggtctgct cgaagccgac cgcgcggtga ccggtacgga
    17101 tgatgccggc gggcagatgc gcagcgagaa actcgatgta gtcggcgcgg tgcatgccaa
    17161 aacaggcatt ccaccccgcg gcgtcggtca cctgaactgg cgcgatcgga gcgccgtcat
    17221 ggcggaaata cgcggatccc gggccgacac gggcgcccca gcgctcaacc gcgccgccga
    17281 acccgatgct gtcgaggtgt cgtaccgcgt ttggcgtgac gtagacaccg gcgccgatct
    17341 cgccgatcgc cgtagcctgc tcgtagacgg caacgcgcag cccgcgcgcg agcagcgcgt
    17401 tggccgcgaa caggccgccg atgccgccgc cgacgatcgc gacactgggt cgggtaacca
    17461 tgcccgtatt gtgcggctac tagcgagtct cgcaacgctg tttcgaaggc cgcaccagcc
    17521 ggatccctag aattccgcca tgccccgagg ataggcaaca tgaacgccct ccggcagcgg
    17581 ccgccctggc ttgggtcccg tccgtccacg atcgtcgacg cccaccttgg ccatgccctg
    17641 gccgggctca ctccattcgg caaagaaggc cagcccggcg gccaccgaca ggatcatggc
    17701 gaagaagtgg ccgaattgct tcaggaacga taccagccgc gatccgccat ctagcggctc
    17761 ggaaaaccct accgaccgtc ggaagagttt gcccggttca ccgtgggccc cgcgaccgac
    17821 ccggtcgcga agtccgcgta gttcctgggg gtgaggagtg gcacggatga tgccatccca
    17881 gaggttcatg agccgcggac gcggactgct cgtcttgctc gtgctcctcc tcgtcgtcgc
    17941 ggggatcctg atctatcgac tgccggccgc gaccccggtg agagaaatgc tgcgctcggt
    18001 gaaagacaca tcgcaggacg cggcgaccac ctccaaggtc aagaccgccc tgctgctctc
    18061 caagcacgtg tcggccttcg acatcaaggc cacgaccact cgcggggtcg tcagcgtgac
    18121 cggggaagtg cccagcgagg aaacacggcg gctcgccggc gccatcgcgc aggacacgtc
    18181 cggggtggcc cacgtccaga acgacctgtt ggtcaatccc ggcgccggcc gcaattccgc
    18241 gatggcgaac ctgggcgacc gcatcgccga cctcgaggtc aagaccatcg tcatcgaccg
    18301 actcgcccgc gcgcccgggc tcaaggacaa gcgcctcacg gtgcaggtcg cgcggcaggt
    18361 cgtgacgctc gaggggacgg tcgactcgcc ggcgcagaag cgggcggccg aggacatcgc
    18421 gatccaggcc cccggggtcc agagcctcgt cagccagatc accgtcgcgg ccggccaggc
    18481 gggcccggag ag
//
LOCUS       DASUFA010000037         5261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1038731, whole genome shotgun sequence.
ACCESSION   DASUFA010000037 DASUFA010000000
VERSION     DASUFA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038731"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFX87_01370"
     CDS             <1..798
                     /locus_tag="VFX87_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4993581.1"
                     /translation="AVHMALAYLIDPRTPKNEGTFRPVTVKARQGTVVWPYPPAPVTL
                     STNHCAQEIAEAMIKALAKSCPDRVLAGWSRRFRIAISGTNPRNKRKFIWHLFHARGG
                     GGASAAGDGWETAGEGQAAGGIKFGSIEVAETRFPLFFKRHEFRPDSAGDGRYRGGVG
                     SVLELRMETTEPALGNTAGDGVRYPAYGVLGGKDGLPHRYRMISRGKTRVLKTKEVGI
                     PIPPGAVFLVESMGGGGYGPPARRSAEARAADRVNGFVSAKPRRGRA"
     gene            800..2836
                     /locus_tag="VFX87_01375"
     CDS             800..2836
                     /locus_tag="VFX87_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4993582.1"
                     /translation="MLRVGVDVGGTFTDLVGVDDAGRVSLAKSASTPADPSIGVMDGL
                     GLLAAELGTDLAGLLADTGLIVHGTTVATNALLERKGAKVGMLTTAGHRDVIEMREGL
                     KDDRYNLRMPPPVPLVPRARRLDVTERMRFDGSVETPLSKTSAEAAIRRLGAAGVGAV
                     AVCYLHSYRDPRHERETRKLLARLLPDAYVSLSSEVLAQIKEYERFCTTVVNAYVGPV
                     LGRYLGNLGSRLRKAGYQGDVLIMQSHGGVATIADSVRLAAGAVLSGPAGGIAGSRHA
                     ARLLGEGNLITFDMGGTSTDIALLEGGEPHLTGDKAVGSSKVALPSIDIHTLGAGGGS
                     IARVDAGGILHVGPESAGAVPGPACYGRGGTAATVTDANLVLGLLDPAGFLGGRARLD
                     PAAAQTAVEGVAARLGLGLLPAAEGIHRVVNTNMAEGIRLVSVRRGADPRRFTLLSFG
                     GAAGLHITEVARMLEINRVVVPRVASVLSAWGMLATDLRYEFVRTHVGEAGQVGAGRL
                     RKLFAEMEAEGRQRLGASFAGHVDVRRSVDMRYGEQTFEIQVPLDGVDVEAEDLMDQV
                     VERFHKRHEELYTYSAPGQDVVLVNVRAAVIGRLPALPAEQLRRVGAAAPPRSRRVYL
                     ERWMEIPVYDMDGVQHGQEVKGPAIFESATTTVLIRARERAVVTAEGWLDIRLS"
     gene            complement(2902..4593)
                     /gene="ilvD"
                     /locus_tag="VFX87_01380"
     CDS             complement(2902..4593)
                     /gene="ilvD"
                     /locus_tag="VFX87_01380"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX4993583.1"
                     /translation="MAVDPRKHSRVLLEGTDRSAARAMMKAVGFSDRDLSQPQIGVAH
                     SWIGTMPCNWNHRKLAEKVMQGIRAAGGTPIEVNTISITDGITMGTEGMKGSLISREV
                     VADSVELVARSHMFDGLVTISGCDKTIPAMTMVLGRLDIPGLMLYCGSIMFGRCAGAT
                     GPFANRNLTIQDVFEAIGAYNAGKISLEQFRDVEDHACPGAGACGGQFTANTMSTAYE
                     MLGVSPMGWNDIPATDPRKEEVAFESGKLVMDLVRKGLTPRKIVTRKSLENSIAGVMA
                     TGGSTNAVLHLLATARDFGLTLSIEDFDRISRKTPVLADLKPWGNYTAPEMYDAGGMA
                     VVGKRLLEAGRLHASEKTVTGRTIGEEVRAAAEPAGQQVIKPLAKALKSEGGIAILRG
                     NLAPGGCVIKLSGQKKYSHRGPARVFEVEEDAFQAIKKGKIKPNDVIVIRYEGPKGGP
                     GMREMLHVSGALQGAGLGESVALMTDGRFSGATHGFMIGHIVPEAADRGPIAAVRNGD
                     IINIDVKKRRLDLEVPAAEMKKRLAKWKAPKPRFKTGVFAKYAATVSDASVGAYT"
     gene            complement(4631..>5261)
                     /locus_tag="VFX87_01385"
     CDS             complement(4631..>5261)
                     /locus_tag="VFX87_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4993584.1"
                     /translation="TWGSHLLTVACVWRGPRERRGVALTFDDGPDPECTPRVLDILER
                     EGVRGAFFMIGQRAERAPGVARRIAEAGHDLGNHTWSHTSLWRCGPDRTEREIGDGHA
                     AITQAAGAPPRFFRPPWGKTNLAMFGALRRLETPCVFWTVQPESRRPVDPAEQARRGA
                     ARARPGAIYDLHDADGVPGAGRRLVEYLPALIADLRGRGYALVPLRDLL"
BASE COUNT          707 a         1827 c         1951 g          776 t
ORIGIN      
        1 gcggtgcaca tggcgctggc ctacctgatc gacccgcgca cgcccaagaa cgagggcacg
       61 ttccggccgg tgaccgtgaa ggcgcgccag ggcacggtgg tgtggcccta cccgcccgcg
      121 ccggtgacgc tgtccacgaa ccactgcgcg caggagatcg cggaggccat gatcaaggcc
      181 ctcgcgaaga gctgcccgga ccgcgtgctc gcgggctggt cgcgccgctt ccgcatcgcg
      241 atctcgggca cgaatccacg gaacaagcgc aagttcatct ggcatctctt ccacgcgcgc
      301 gggggcggag gcgcgtccgc cgcgggcgac ggctgggaga ccgcgggcga gggccaggcg
      361 gcggggggca tcaagttcgg cagcatcgag gtcgcggaga cgcgcttccc gctcttcttc
      421 aagcggcacg agttccgccc ggactcggcg ggcgacggcc gctaccgcgg cggcgtggga
      481 tcggtgctcg agctgcgcat ggagaccacg gagcccgcgc tcggcaacac cgcgggcgac
      541 ggggtccgct acccggccta cggggtgctg ggcggcaagg acgggctccc ccatcgctac
      601 cggatgatct cgcgcggcaa gacgcgcgtg ctgaagacga aggaggtcgg gatcccgatt
      661 ccgccgggcg ccgtcttcct ggtcgagtcc atgggcggcg gcggctacgg gccgccggcg
      721 cgccgctccg ccgaggcgcg cgccgccgac cgcgtcaacg gcttcgtctc cgcgaagccg
      781 cgccgcggga gggcctgaga tgctgcgcgt cggcgtcgac gtcggcggga ccttcaccga
      841 tctggtcggg gtggacgacg ccgggcgcgt gagcctcgcc aagtccgcct ccactcccgc
      901 ggacccgtcc atcggcgtca tggacggcct cggcctgctg gccgcggagc tgggcaccga
      961 cctggccggg ctgctggccg acacgggcct catcgtacac gggaccaccg tggccaccaa
     1021 cgcgctgctc gagcgcaagg gcgcgaaggt cgggatgctg accaccgcgg ggcaccgcga
     1081 cgtgatcgag atgcgcgagg gcctcaagga cgaccgctac aacctccgca tgccgccacc
     1141 cgtgcccctg gtgccgcgcg cgcgccggct cgacgtgacc gagcggatgc gcttcgacgg
     1201 cagcgtggag acgccgctct cgaagacgtc ggcggaggcg gcgatccgcc ggctgggcgc
     1261 cgcgggagtc ggcgcggtcg cggtctgcta cctgcactcc taccgggatc cgcgccacga
     1321 gcgggagacc cggaagctgc tcgcgcggct cctgccggac gcctacgtgt cgctctcctc
     1381 cgaggtgctc gcgcagatca aggagtacga gcgcttctgc accaccgtgg tgaacgctta
     1441 cgtgggcccg gtgctgggcc gctacctggg caatctgggc tcgcgcctgc gcaaggcggg
     1501 ctaccagggc gacgtgctga tcatgcagtc gcacggcggg gtcgccacca tcgccgactc
     1561 ggtgcgcctg gccgcgggcg cggtgctctc gggcccggcc ggcggcatcg cgggaagccg
     1621 gcacgcggcg cgcctgctcg gcgagggcaa cctcatcacg ttcgacatgg gcgggaccag
     1681 caccgacatc gcgctgctcg aaggcggtga gccgcacctc accggcgaca aggcggtggg
     1741 gtcgagcaag gtcgcgctgc cgagcatcga catccacacg ctcggcgcgg gcggcggctc
     1801 catcgcccgg gtggacgcgg gcggcatcct ccacgtgggg cccgagagcg cgggcgcggt
     1861 gcccgggccc gcgtgctacg ggcgcggagg caccgcggct accgtcaccg acgccaacct
     1921 ggtgctgggc ctgctcgacc cggcgggctt tctcggcggg cgcgcccgcc tggacccggc
     1981 ggcggcgcaa accgcggtgg aaggcgtggc ggcgcggctc ggcctcggtc tgctgccggc
     2041 ggccgaaggg attcaccgcg tggtcaatac caacatggcg gagggcatcc ggctggtctc
     2101 ggtgcggcgg ggcgccgacc cgcggcgctt cacgctgctc tcgttcggcg gcgcggccgg
     2161 gctccacatc acggaggtcg cgcgcatgct cgagatcaat cgcgtcgtgg tgccgcgcgt
     2221 ggcctcggtg ctctcggcct ggggcatgct ggccaccgac ctgcgctacg agttcgtgcg
     2281 cacgcacgtg ggcgaggccg gccaggtcgg cgcggggcgc ctgcgcaagc tcttcgccga
     2341 gatggaggcg gagggacggc agcgactggg cgcgtcgttt gcgggccacg tggacgtgcg
     2401 tcgctcggtg gacatgcgct acggcgagca gaccttcgag atccaggtgc cgctcgacgg
     2461 ggtggacgtc gaggcggagg atctcatgga ccaagtagtc gagcgcttcc acaagcgcca
     2521 cgaggagctt tacacctaca gcgcccccgg ccaggacgtg gtgctggtca acgtgcgtgc
     2581 cgcggtgatc gggcggctcc cggccctgcc cgccgagcag ttgcggcgcg tgggcgcggc
     2641 ggccccgccg cggagccggc gcgtgtatct cgagcgctgg atggagatcc ccgtgtacga
     2701 catggacggg gtgcagcacg gccaggaggt gaagggcccc gccatcttcg agtcggccac
     2761 caccaccgtg ttgatccggg cgcgcgagag ggcggtcgtc accgcggaag ggtggttaga
     2821 tatccggctg agctagcgct gcgagaccgc ccctcaccct gccctctccc ctgaggggag
     2881 agggtcatcg gggggaacgg cctatgtgta ggcgccgacc gaggcgtcgg agacggtggc
     2941 cgcgtacttg gcgaagacgc cggtcttgaa gcgcggcttg ggcgccttcc acttggcgag
     3001 gcgcttcttc atctcggcgg ccggcacttc cagatcgagg cgccgcttct tcacgtcgat
     3061 gttgatgatg tcgccgttcc gcaccgccgc gatggggccg cggtcggccg cctccggcac
     3121 gatgtggccg atcatgaagc cgtgggtggc gcccgagaag cggccgtcgg tcatcagggc
     3181 cacgctctcg cccagccctg ctccctgcaa ggcgcccgag acgtgcagca tctcgcgcat
     3241 gccggggccg cccttgggtc cctcgtagcg gatcacgatc acgtcgttgg gcttgatctt
     3301 gcccttcttg atggcctgga atgcgtcttc ctcgacttcg aagacccgcg cgggcccgcg
     3361 gtgcgagtac ttcttctggc cggagagctt gatcacgcag ccgccggggg ccaggttgcc
     3421 gcgcaggatc gcgatgccgc cctcggactt gagcgccttg gcgagcggct tgatcacctg
     3481 ctggcccgcg ggttccgcgg ccgctcggac ctcctcgccg atggtgcgcc cggtcaccgt
     3541 cttctcgctc gcgtggagcc ggccggcctc gagcaggcgc ttgcccacca ccgccatgcc
     3601 gcccgcgtcg tacatctcgg gcgcggtgta gttgccccac ggcttgaggt cggcgagcac
     3661 cggcgtcttg cgcgagatcc gatcgaaatc ctcgatcgag agtgtgaggc cgaagtcgcg
     3721 ggcggtggcc aggagatgca gcaccgcgtt ggtggagccg ccggtggcca tgactcccgc
     3781 gatggagttc tcgaggctct tgcgggtgac gatcttccgc ggggtcagtc ccttgcgcac
     3841 gagatccatg accagcttgc cggactcgaa cgccacttcc tccttgcgcg ggtccgtggc
     3901 cgggatgtcg ttccagccca tcggggacac gccgagcatc tcgtaggcgg tggacatggt
     3961 gttggcggtg aactggccgc cgcaggcgcc cgcgccgggg caggcgtggt cctccacgtc
     4021 ccggaattgc tcgagcgaga tcttccccgc gttgtaggcg ccgatggcct cgaacacgtc
     4081 ctggatcgtc aggttgcggt tcgcgaacgg gcccgtggcg ccagcgcagc ggccgaacat
     4141 gatcgagccg cagtagagca tgaggcccgg gatatcgagc cggcccagca ccatggtcat
     4201 ggccgggatg gtcttgtcgc agcccgagat cgtgacgagg ccgtcgaaca tgtggctgcg
     4261 ggccaccagc tccaccgaat cggccacgac ctcacggctg atcagcgagc ccttcatgcc
     4321 ctcggtgccc atcgtgatgc cgtcggtgat cgagatcgtg ttgacctcga tcggcgtgcc
     4381 gcccgcggcc cggatcccct gcatgacctt ctcggccagc ttccggtggt tccagttgca
     4441 gggcatggtg ccgatccagg agtgggcgac accgatctgg ggctgtgaaa gatcgcggtc
     4501 gctgaagccc accgccttca tcatggcgcg ggcggcggag cgatcggtgc cttcgaggag
     4561 aactcggctg tgcttgcggg gatcgacggc catcggggac tcctttgtcg tgggcttggt
     4621 cgggggaagt ctacagcagg tcgcggagcg ggaccagcgc gtagccccgc ccgcggagat
     4681 ccgcgatcag cgcgggtagg tactcgacga gacgcctccc cgccccgggc acgccgtccg
     4741 cgtcgtgcag atcgtagatc gcgcccggcc gggcgcgggc ggcgccgcgt cgagcctgct
     4801 ccgcgggatc caccggccgg cggctttccg gctgcaccgt ccagaacacg cacggggtct
     4861 cgagccggcg cagcgcgccg aacatggcga gattggtctt gccccacggc ggccggaaga
     4921 agcgcggcgg cgcgccggcg gcctgggtga tggccgcgtg cccgtcgccg atctcccgct
     4981 cggtccggtc gggcccgcag cgccagaggc tcgtgtggct ccaggtgtgg ttgccgagat
     5041 cgtggccggc ctccgcgatg cgccgggcga cccccggcgc ccgctcggcg cgctggccga
     5101 tcatgaagaa ggcgccgcgg acgccctcgc gctccaggat gtcgagcacg cgcggcgtgc
     5161 attccggatc cggcccgtcg tcgaaggtga gcgccacgcc ccggcgctcc cgcgggccgc
     5221 gccacacgca ggccaccgtg aggaggtgcg agccccaggt a
//
LOCUS       DASUFA010000038         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_466391, whole genome shotgun sequence.
ACCESSION   DASUFA010000038 DASUFA010000000
VERSION     DASUFA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466391"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFX87_01390"
     CDS             complement(<1..347)
                     /locus_tag="VFX87_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993585.1"
                     /translation="MKRLKASAIGAAALLLTAGGTLAQSRVDQTQLGGSHAAAGADIR
                     TVEGKVVDLERRPDMVTMLRLDNGASLQVTAESQGPGQPAQVGDSIMARYAESGGRDK
                     IATQVRVIETQAP"
     gene            554..727
                     /locus_tag="VFX87_01395"
     CDS             554..727
                     /locus_tag="VFX87_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993586.1"
                     /translation="MRRRYRRLARRFRSIAFIATVFRTYREGRRRGQTRLTALRFGRA
                     VAQWQRHNGAAHS"
     gene            735..1184
                     /locus_tag="VFX87_01400"
     CDS             735..1184
                     /locus_tag="VFX87_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993587.1"
                     /translation="MLRLVLAGLAAATMPAVARAQTPAPGAPAPEPVTRELQQALAAA
                     TQRFQALDSPGVLAHVSDQYRNGPFTKPALREHLLAMFTLYDTVRARVRVDEIRLVDG
                     AAWVYSTGEISGRLRGIGVWTSALTWEREPDVARKENGAWRLVGPGP"
     gene            complement(1185..1535)
                     /locus_tag="VFX87_01405"
     CDS             complement(1185..1535)
                     /locus_tag="VFX87_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993588.1"
                     /translation="MHWTNWRMTFPSLPGQQERVARETVAELRAGRPVQLFVTDFPRI
                     KFHHSVLAFDYRADGSDVVDPDAPGIVRFDPRDEHFHAAPLCGVSVPYVRTFRRYYFA
                     VHLGRLAAGPLSAP"
     gene            1663..>3506
                     /locus_tag="VFX87_01410"
     CDS             1663..>3506
                     /locus_tag="VFX87_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX4993589.1"
                     /translation="MLRRVALRRPREDPDLTAVLLLYALTLFAGATLLFVMQPMVGKM
                     ILPLLGGTPAVWSTCMVFFQAALLGGYAYAHASAARLRPSRQALLHLFVLAVPLAVLP
                     LAVNPRLLRGGETNPVLDALTLLSVSVGLPFLVVSATAPLLQKWFTQTGHPAGRDPYF
                     LYAASNLGSMLALLGYPTLVEPRLPLQGPSWLTQTTLWSGGYGVLAALTALCALTVWW
                     TPADAVTAVPEEAPDAPAEPAPGWRRRLHWIALALVPSSLLLGATTYITTDLAAVPLL
                     WVLPLAIYLLTFILAFGRWPRVLHRAVVAIALPLVLVVIFLMVSALPERIWVTVLWHF
                     GVLFVLALACHGALALDRPAPRHLTEFYLLISVGGVLGGLFNALLAPLIFSSRIEYPL
                     VMVLACVLLRAGRGDAGLGLGPGARRTAALVLLIAALALVLYSESATLRVDFSFLARV
                     LEWSSARVGERLDPIERPLNRVLIYGPPLALAWLLRRRPLHLGLALAAVLLVAGFVDE
                     RNNDQIRQSRSFFGVLRISRDRDPTGYTELRHGTTLHGRQSLEPSRRAEPISYYSRKG
                     PIGQLFAELDRRPGAVRMAVIGLGTGTLAAYARPGDRVTFYEIDRLVR"
BASE COUNT          435 a         1339 c         1188 g          544 t
ORIGIN      
        1 cagggcgcct gcgtctcgat cacccggacc tgcgtcgcga tcttgtcgcg cccgccgctc
       61 tcggcgtagc gcgccatgat cgagtccccc acctgggccg gctggcccgg cccctgactc
      121 tcggccgtca cctggagcga cgcgccattg tcgagcctca gcatcgtcac catgtcgggg
      181 cgccgctcga gatccacgac cttgccttcg acggtccgga tgtcggcccc ggccgcggcg
      241 tgcgagccac cgagctgggt ctgatccacg cgcgattgcg cgagcgtgcc gccggcggtg
      301 agtaggaggg ctgcggcccc gatcgcgctg gctttcagac gcttcatgac agctcctttc
      361 ttgaatggaa gtccctaccg cggggcgttg gccccgcttc cgcataagga cacagcaagg
      421 gcgataccag atccggcaat cgcggggtcg aaggcccgat tgggaagccc cggggcttat
      481 caggatgtaa ggcttacacg cgctgggaac gtaagatgtt ccagtgacgt tccacgttct
      541 aggaggaggc gagatgcgac gacgctacag acggctggcc cggcggttcc gatccatcgc
      601 cttcatcgcc accgtgttcc gcacctaccg tgagggccgc cggcggggcc agacccgcct
      661 gaccgcactg cgcttcggcc gcgcggtggc ccagtggcag cgccacaacg gggccgcgca
      721 ttcgtagacg ctcggtgctc cgcctggtcc tcgccggcct cgcggccgca acgatgcccg
      781 cggtggcccg cgcccagacc cccgcgcccg gcgcgcccgc gccggagcca gtgacgcgcg
      841 agctgcagca ggcgctcgcc gccgccaccc agcgcttcca ggccctggac tcgcccggcg
      901 tcctcgccca cgtgtccgac cagtaccgca acggcccgtt caccaagccg gcgctccgcg
      961 agcacctgct ggccatgttc accctttacg acacggtgcg cgcccgcgtc cgcgtggacg
     1021 agatccgcct cgtggacggc gccgcgtggg tctactcgac cggcgagatc tcgggacgcc
     1081 tccgcggcat cggggtgtgg acctccgctc tgacctggga acgcgagccc gacgtcgccc
     1141 gcaaggaaaa cggcgcctgg cgtctggtcg gccccggccc ctgactaggg cgccgacaag
     1201 ggcccagctg cgaggcggcc tagatgaacg gcgaagtagt accggcggaa ggtgcggacg
     1261 tagggcaccg agacgccgca gagcggcgcg gcatgaaagt gctcgtcgcg cgggtcgaag
     1321 cggacgatcc ccggcgcgtc cggatccacc acgtccgaac cgtccgcgcg atagtcgaag
     1381 gccagcaccg agtgatggaa cttgatgcgc gggaagtcgg tcacgaagag ctgaaccggc
     1441 cgccccgccc gtagctcggc caccgtctcg cgggccaccc gctcctgctg gccgggcagc
     1501 gacgggaacg tcatccgcca gttcgtccag tgcacgagcg tccagaagcg cccgcgcagc
     1561 ccatccttga ccgcggccgc ctccgcgcgg gagaaggcgt ggagcgacgc gtacccgacg
     1621 gcggacgggg aagggcgagc gtcgcgccga tgattatacg gtatgctacg ccgcgtcgcc
     1681 ctgaggcggc cacgggagga tcccgatctg accgccgttc tgctgctcta cgcgctcacc
     1741 ctcttcgcgg gcgccacgct cctgttcgtg atgcagccga tggtgggcaa gatgatcctg
     1801 ccgctgctcg gcggcacgcc cgcggtgtgg agcacctgca tggtgttctt ccaggccgcg
     1861 ctcctcggcg gctacgccta cgcgcacgcg agcgccgcgc gcctgcggcc ctcgcgccag
     1921 gcgctcctgc acctgttcgt gctcgcggtg cccctggccg tcctgcccct ggccgtgaac
     1981 ccgcgcctgc tgcggggcgg cgagaccaac ccggtgctcg acgcgctcac gctcctgtcg
     2041 gtctcggtgg ggctgccctt cctggtggtg agcgcgaccg cgccgctgct ccagaagtgg
     2101 ttcacccaga ccgggcaccc ggccgggcgc gatccctact tcctctacgc ggccagcaac
     2161 ctcggcagca tgctggcgct gctgggctac ccgaccctcg tcgagccgcg cctgccgctg
     2221 cagggcccga gctggctcac ccagaccacg ctgtggagcg ggggttatgg cgtgctcgcg
     2281 gcgctgaccg cgctctgcgc actgaccgtc tggtggacgc cggcggacgc cgtgaccgcg
     2341 gtcccggagg aagcgccgga cgcgcccgcc gagcccgcgc ccggctggcg acgccggctc
     2401 cactggatcg ccctggccct cgtgccctcg agcctgctgc tcggcgcgac cacctacatc
     2461 acgaccgacc tggcggcggt gccgctcctg tgggtgctgc cgctcgcgat ctacctgctg
     2521 accttcatcc tcgccttcgg ccgctggccg cgggtgctcc accgcgcggt cgtggcgatc
     2581 gccctgcccc tcgtcctggt ggtgatcttc ctgatggtct cggcgctgcc cgagcgcatc
     2641 tgggtcaccg tgctctggca cttcggggtg ctcttcgtgc tcgcgctcgc ctgccacggc
     2701 gcgctcgccc tcgatcgccc cgccccgcgc cacctgaccg agttctacct gctgatctcg
     2761 gtgggcggcg tgctcggcgg cctgttcaac gcgctgctcg ccccgctgat cttctcctcg
     2821 cggatcgagt acccgctggt gatggtgctg gcctgcgtgc tcctccgcgc gggtcgcggc
     2881 gacgcggggc tcgggctcgg ccccggggcg cggcggaccg cggccctcgt gctgctgatc
     2941 gcagccctcg ccctcgtcct ctactcggag tcggccacgc tccgcgtgga cttctcgttc
     3001 ctggcccgcg tgctcgagtg gagcagcgcg cgggtcggcg agcggctcga tccgatcgag
     3061 cggccgctca accgagtcct gatctacggc ccgccgctgg cgctcgcctg gctcctgcgg
     3121 cgccggccgc tccacctggg gctggcgctg gccgccgtgc tgctggtggc cggattcgtg
     3181 gacgagcgca acaacgacca gatccgccag agccgctcct tcttcggcgt gctccgcatc
     3241 tcgcgcgacc gcgacccaac cggctacacc gagctgcgcc acggcaccac gctccacggg
     3301 cggcagagcc tcgagccgtc gcgtcgcgcc gagccgattt cctactactc gcgcaagggc
     3361 cccatcggcc agctcttcgc cgagctcgac cgccgcccgg gcgcggtgcg catggcggtg
     3421 atcgggctcg gcaccggtac cctggccgcc tacgcgcggc cgggcgaccg cgtgaccttc
     3481 tacgagatcg accgtctcgt gcgcga
//
LOCUS       DASUFA010000039         7683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1607811, whole genome shotgun sequence.
ACCESSION   DASUFA010000039 DASUFA010000000
VERSION     DASUFA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607811"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..2880
                     /locus_tag="VFX87_01415"
     CDS             169..2880
                     /locus_tag="VFX87_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4993590.1"
                     /translation="MQNVSLDRLRQVANLARSVASALDVDTVLRQVVEAVLSLRPRAF
                     CVVRLVDMERGGYRLAAKGGVHEDVADVESVDEFIPFGRGVTHVVAQSGQPVMLPDAL
                     GDPRLVSAMWREERGLKVYYGVPISAGGELMGVLSVALSETAPPTAEEREIVDLLASH
                     AAVAIGNARLFAESETRRRTAEALAEVWRFLSETFDVAALGQRIADVVLRALRVRAAV
                     LYRLLPGPGDLAVIGRSGEAERFFDPGVVIPAGHGVVGLAVNRRQPVVTPDLRVDTRI
                     AWPPGMRAGQTLCPSVLSLPLVVKGQVVGGFTVGDFLGRVYTDEDLRLAQTFADQAAV
                     ALENARLFAETERRRRESVALGNVSQTLAQSLDPAGVAQRVVDCVRDLVGVLAASVFR
                     LDPESGALEETASSGVSLFRERPRFSRGIGAVGLAVAERRAVVSNNVLADPRLRFPPE
                     VRASLEISQIRAILAVPLTVKEQVIGALGLLDARGRVFTEDDVRLAQAFADQAAVALE
                     NARLYADAERRRVEAETLSGLVRTITATLDPALVLRRVAAAARELCGSDSAHILLRDA
                     ETDTMFARYGVGQAMGDGDAPRIARGRGAGGQAWSTGRPFRTANRPDDPRVRDDYPEA
                     IRREGVIATLVVPIWISGQVEGLLYVSNRADRPFTDRDEAILIRLADHAAIAIQNATL
                     YSRLQGLSGRLMEVQEAERRHLARELHDEIGQLLTGLRLTLDVPEPPAPALGERLGQA
                     QALVQELLERIRALSLDLRPSILDDLGVLPALLDNIERYMSQTKIRVHLEHSGLDRRF
                     DPETETGVYRIVQEALTNVARHGHVDEVTVRLWATDDVLGVQVEDHGAGFDPDAVLGA
                     GRSGGLAGLRERAALLNGHLTVETHPGRGVRLTAEVPLNGSLRRAGE"
     gene            2880..3536
                     /locus_tag="VFX87_01420"
     CDS             2880..3536
                     /locus_tag="VFX87_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4993591.1"
                     /translation="MKTISVVLAEDHHVVRQGLRALLAAEPDLEVVGEAASGLEVGDL
                     VERVKPDVLVVDFVMPGLNAPDIIRRVARVSPRTRALVLSMHANPAYVVEAIQSGAAG
                     YVLKGSSAAELVRAIRAVAEGSRHLSPPLSEEMVEAYAQRAVPTGLDLYRTLTQRERE
                     VLHLAAEGQTSGQIGARLNISPRTVEVHRANLLRKLRLRGQSDLVRYAVGRGILTVDD
                     "
     gene            3643..4032
                     /locus_tag="VFX87_01425"
     CDS             3643..4032
                     /locus_tag="VFX87_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993592.1"
                     /translation="MPDPETPPATILVVEDEEEVRALARDVLEMNGFRVLEALDVADA
                     TRLAQTHPGPIDLLITDVVMPGASGPELARRLRAQRPGLRVLCMSGYPESEDRRIDGE
                     AGWTAWLQKPFTPDVLMRKVRDCLATS"
     gene            complement(4022..4273)
                     /locus_tag="VFX87_01430"
     CDS             complement(4022..4273)
                     /locus_tag="VFX87_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993593.1"
                     /translation="MYHPTIFFDPQFTIGVMAGWLLQAAGVGALLLAALWFSIAGEWR
                     RGTPAPTAFRALTYLGLVMFLGGILWQFVGYFGTGTLSW"
     gene            complement(4288..5055)
                     /locus_tag="VFX87_01435"
     CDS             complement(4288..5055)
                     /locus_tag="VFX87_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX4993594.1"
                     /translation="MQIAGSTTVITGGASGLGRATAERLHGGGGAVVLLDLATSPGAQ
                     VAAALGERALFAPADVTNPDEVGAALDAAVARFGAVTALVNCAGIGTADKTFGKRGPA
                     DLAGFARTIQVNLIGTFNCIRLAAARMAKNAPNAEGERGVVINTASVAAFDGQIGQAA
                     YSASKGGVVGMTLPIARDLAELGIRVRTIAPGLFETPMLAGLPEAARISLGKQVPFPP
                     RLGRPVEYAALAAHIIENPMLNGEVIRLDGAIRMQPR"
     gene            5115..6251
                     /locus_tag="VFX87_01440"
     CDS             5115..6251
                     /locus_tag="VFX87_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX4993595.1"
                     /translation="MPLPLWIRTPDDLQALARELEGTGALAIDTEADSLHHYPGKLCL
                     VQLADARGRGHLVDPLALRDLSPLGAALADPKTLKVLHAADNDLAYLKRLYGVTVVSL
                     FDTAVAARFLGAPALGLEGLLTQYLGVTAVKSRQKDDWSRRPLTPEQEAYALDDVLHL
                     IPLRERLLEALHAIGRAQWVEEECAGLAALVVPEKPADPDAYMKIKGARELDARGLAV
                     LRELYGAREILALRLDRPPFMIVGNESLVVLAARRPLDAEGILAVPGCTPPVVRRAGP
                     AILDAIARALALPDAELPAYRPAPRPHVPAAQRRRGEALREWRAKASKEIGLEPGVLF
                     PQRLIDRLAAAPPADLAALRQVEGVRDWRVGLFGADLLKALAVA"
     gene            6350..7306
                     /locus_tag="VFX87_01445"
     CDS             6350..7306
                     /locus_tag="VFX87_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993596.1"
                     /translation="MASDSRYFREGIIAGLIGAALVAVWFLIYDAAIGRPFRTPALLG
                     AATFRGVTDPAAVPTAASLVVQYTVLHGVVFALIGVLIAYLIASAQREPSRLLTLFIA
                     LMCFEVFFLAVLTWLAHPVLGELAWWAVLVGNALAAGGMLIYFFVGHRALGRALLGPL
                     WTRVVREGIVAGLLGAGAVALWFLAYDAAAGAPLRTPALLGAALFHGLREPGALVITM
                     PLVLQYTVVHGAVFVLFGLAAAGMLALADREPRLLFAFVMLLCCFEVFFAAMVAILAE
                     WLLEAVPWWTILAGNLLAALVMMGYFLRGHRVTWHEFIHARR"
     gene            7452..>7683
                     /locus_tag="VFX87_01450"
     CDS             7452..>7683
                     /locus_tag="VFX87_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993597.1"
                     /translation="MADGALKGRVAVVTGGNGGIGLGMARGLAGAGAAVVVSGRNAEK
                     SRGAVAELTKLGAAAVAIEADVADEGAVNALVR"
BASE COUNT          965 a         2729 c         2860 g         1129 t
ORIGIN      
        1 gtgggtttct gcggccacgg cttccagcat tcccccgggg tgggcgagat cgtggccgag
       61 gtgctgctcg agggccgctc gacgctcgac atccaggatc tgcgcctcga ccgtttctcg
      121 gcggcttgac acggcggcac cggaccgcta acgtccaagt ccgcagcgat gcagaacgtt
      181 tccctcgacc gtctccggca ggtggcgaac ctggcccgtt ccgtggccag cgccctcgac
      241 gtggacaccg tcctccgcca ggtcgtggag gcggtgctct cgttgcgccc gcgggccttc
      301 tgcgtggtgc gcctggtgga catggagcgg ggtggctacc ggctcgcggc caagggcggc
      361 gtccacgaag acgtggcgga tgtcgagagc gtcgacgagt tcatcccgtt cgggcgcggg
      421 gtcacccacg tcgtcgcgca gagcgggcag ccggtgatgt tgcccgatgc gctcggcgat
      481 cctcggctcg tgtcggcgat gtggcgcgag gagcgcggcc tcaaggtcta ctacggggtg
      541 ccgatcagcg cgggcggcga gctgatgggc gtgctcagcg tagcgctctc cgagaccgcg
      601 ccgcccaccg cggaggagcg ggagatcgtg gacctgctgg cgagccacgc ggcggtcgcg
      661 atcggcaacg cgcggctctt cgcggagagc gagacgcggc gccggaccgc ggaggctctc
      721 gcggaggtct ggcgcttcct ctccgagacc ttcgatgtgg ccgcgctcgg ccagcgcatc
      781 gccgacgtgg tgctgcgcgc cctgcgggtg cgcgccgcgg tgctctaccg cctcctgccc
      841 ggcccgggcg atctcgcggt gatcggccgc tcgggggagg cggagcggtt cttcgatccc
      901 ggggtcgtga ttcccgcggg ccacggcgtg gtcgggctgg ccgtgaaccg ccggcagccg
      961 gtggtcacgc ccgacctgcg ggtggatacc cgcatcgcgt ggcccccggg catgcgcgcg
     1021 ggtcagaccc tgtgcccgtc ggtgctctcg ctgccgctgg tggtgaaggg gcaggtggtg
     1081 ggtggcttca cggtgggcga cttcctcggc cgcgtctaca ccgacgagga cctgcggttg
     1141 gcccagacct tcgcggacca ggccgcggtg gcgctcgaga acgcgcggct cttcgcggag
     1201 acggagcggc gacgccgcga gagcgtggcg ctgggcaacg tgagccagac gctcgctcag
     1261 tcactcgatc ccgccggcgt cgcgcagcgg gtggtggact gcgtgcggga cctggtcggc
     1321 gtgctcgcgg cctccgtgtt ccgcctcgat cccgagtcgg gcgcgctgga ggagacggcg
     1381 tcctcggggg tgtcgctctt ccgcgagcgg ccgcggttct cgcgcggcat cggcgcggtg
     1441 gggctggcgg tcgccgagcg gcgggcggtg gtctccaaca atgtcctcgc cgatccgcgg
     1501 ctgcgcttcc cccccgaggt gcgcgcgtcc ctcgagatct cgcagatccg cgccatcctc
     1561 gcggtgccgc tcaccgtgaa ggagcaggtg atcggcgcgc tcggcctgct ggacgcgcgc
     1621 ggccgggtct tcaccgagga cgacgtgcgc ctggcccagg ccttcgcgga ccaggcggcg
     1681 gtggccctcg agaacgcgcg gctctacgcg gacgccgagc ggcggcgggt cgaggccgag
     1741 acgctctccg ggctggtgcg caccatcacc gccacgctcg atcccgccct ggtcctgcgc
     1801 cgggtggccg cggccgcccg ggagctgtgc gggagcgact ccgcccacat cctgctccgc
     1861 gatgcggaga ccgacacgat gttcgcccgc tacggcgtgg gccaggcgat gggcgacggg
     1921 gacgctccgc gcatcgcgcg cggccggggc gccgggggcc aggcgtggag cacggggcgg
     1981 cccttccgga ccgcgaaccg gcccgacgac ccacgggtgc gcgacgacta cccggaggcg
     2041 atcaggcggg aaggcgtcat cgccaccctc gtggtgccga tctggatcag cgggcaggtc
     2101 gaggggctcc tctacgtctc caaccgcgcc gaccggccct tcaccgatcg cgatgaggcg
     2161 atcctgatcc gtctggccga ccacgcggcc atcgcgatcc agaacgccac gctgtacagc
     2221 cgcctgcagg gcctctccgg gcgtctgatg gaggtgcagg aggcggagcg ccgccacctg
     2281 gcccgcgagc tccacgacga gatcggccag ctcctcaccg ggctccgcct caccctcgac
     2341 gtgcccgagc cgccggcgcc cgcgctgggc gagcggctcg ggcaggctca ggccctggta
     2401 caggagctgc tcgagcgcat ccgcgcgctc tcgctcgacc tgcggccgag catcctggac
     2461 gatctgggcg tgctgcccgc gctgctcgac aacatcgagc gctacatgag ccagaccaag
     2521 atccgcgtcc acctcgagca cagcgggctc gaccggcgct tcgacccgga gaccgagacc
     2581 ggcgtctacc gcatcgtcca ggaggcgctg accaacgtgg cccggcacgg ccacgtggat
     2641 gaggtgacgg tgcgcctctg ggccaccgac gacgtgctgg gcgtccaggt cgaggaccac
     2701 ggcgccggct tcgacccgga cgcggtgctc ggcgcgggcc ggagcggcgg gctggccggc
     2761 ctgcgggagc gggccgcgct gctgaacggc cacctcacgg tggagacgca tcccgggcgc
     2821 ggggtacggc tgaccgccga ggtcccgctc aacgggtcct tgcgaagggc cggcgagtga
     2881 tgaagacgat cagcgtggtg ctggccgagg atcatcacgt ggtccggcag ggcctgcgcg
     2941 ccctcctcgc ggccgagccg gatctcgagg tggtgggcga ggcggcgagc ggcctcgagg
     3001 tgggcgatct cgtcgagcgt gtgaagcccg acgtgctggt ggtggacttc gtgatgccgg
     3061 ggctgaacgc gcccgacatc atccggcggg tggcccgggt ctccccgcgc acgcgcgcgc
     3121 tcgtcctctc catgcacgcg aacccggcct acgtcgtcga ggccatccag agcggggccg
     3181 ccggctacgt gctcaagggc tcgagcgcgg cggagctggt gcgggcgatc cgcgcggtgg
     3241 cggagggctc ccgacacctg agcccgccgc tctccgagga gatggtggag gcctacgcgc
     3301 agcgcgcggt ccccaccggc ctcgatctct accgcacgct cacccagcgg gagcgcgagg
     3361 tcctgcatct ggcggcggag ggacagacca gcgggcagat cggcgcgcgg ctgaacatca
     3421 gcccgcgcac cgtggaggtc catcgggcga acctgctgcg caagctgcgg ctacgcggcc
     3481 agagcgatct ggtccgctac gcagtgggac gtgggatcct cacggtcgac gactgagccg
     3541 cgggctggaa ttacgtaccc gtacgaatag acgcgggtgc gctcgcgcgc gcatggttgt
     3601 ggacagtgcg aatctggtcc acggtggtcc gcccgcttcc cgatgcctga tccggagacg
     3661 cctccggcca cgatcctggt cgtcgaagac gaggaagaag tgcgcgcgct cgcgcgcgac
     3721 gtcctcgaga tgaacggctt ccgggtgctg gaggcgctcg acgtggccga cgccacccgc
     3781 ctcgcccaga cccatcccgg accgatcgac ctgctgatca ccgacgtggt gatgccggga
     3841 gcgagcggcc ccgagctggc gcggcggctg cgcgcgcagc gacccggcct gcgcgtgctc
     3901 tgcatgtcgg gctatccgga gagcgaggac cgtcggatcg acggcgaggc cggctggacc
     3961 gcgtggctgc agaagccctt cacgcccgac gtgctgatgc ggaaggtgcg cgactgcctg
     4021 gctaccagct gagcgtgccg gtcccgaagt agcccacgaa ctgccagagg atcccgccca
     4081 ggaacatcac gagcccgagg taggtgaggg cgcggaacgc ggtgggcgcc ggggtcccgc
     4141 ggcgccactc gcccgcgatg gagaaccaga gcgcggccag cagcagcgcg ccgactcccg
     4201 cggcctggag cagccagccc gccatcacgc ctatcgtgaa ctgcggatcg aagaagatcg
     4261 tcggatggta catggtcgct cctgttctca gcggggctgc atgcggatgg ccccgtccag
     4321 gcggatgacc tcgccgttga gcatcgggtt ctcgatgatg tgggccgcga gggcggcgta
     4381 ctcgaccggc cgcccgagcc ggggcgggaa gggcacctgc ttgcccagcg agatccgcgc
     4441 ggcctcgggc aggcccgcca gcatgggtgt ctcgaagagg ccgggggcga tggtcctgac
     4501 ccggatgccc agctcggcca gatcgcgggc gatcggcagc gtcatgccga ccacgccgcc
     4561 cttggacgcg gagtaggcgg cctgcccgat ctgaccatcg aaggcggcca cggaggcggt
     4621 gttgatcacc acgccgcgct cgccctcggc gttgggcgcg ttcttggcca tccgcgcggc
     4681 ggcgagccgg atgcagttga aggtgccgat gaggttgacc tggatggtgc gcgcgaaccc
     4741 cgccaggtcc gcgggcccgc gcttgccgaa ggtcttgtcc gcggtgccga tgcccgcgca
     4801 gttgacgagg gcggtgacgg ccccgaagcg cgccaccgcc gcgtcgaggg ccgcgccgac
     4861 ctcgtcgggg ttggtgacgt ccgcgggcgc gaagagagcc cgctcgccga gggccgcggc
     4921 gacctgggcg cccggcgacg tcgcgagatc gagcagcacc accgcgccgc cgccgccgtg
     4981 caggcgctcc gcggtggccc ggccgagacc ggaggcgccg cccgtgatca ccgtggtcga
     5041 tcccgcgatc tgcatgccgt cctcctcctg ctccagggta ccgtcgccga cggtgtagta
     5101 taaccgagcc gaccatgccg cttcccttgt ggatccggac tccggacgac ctgcaggcgc
     5161 tggcccgcga gctcgaaggc acgggcgcgc tggccatcga caccgaggcc gacagcctgc
     5221 atcactaccc gggcaagctc tgcctcgtgc agctggccga cgcgcgcggg cgcgggcacc
     5281 tggtggatcc gctcgcgctg cgcgacctct cgccgctggg ggccgcgctg gccgatccga
     5341 aaaccctcaa ggtgctgcac gccgccgaca acgatctggc ctacctcaag cggctgtacg
     5401 gcgtcacggt ggtctcgctc ttcgacaccg cggtggccgc ccggttcctc ggcgcccccg
     5461 cgctcggcct cgaagggctg ctcacccagt acctgggcgt caccgcggtg aagtcgcggc
     5521 agaaggacga ctggtcccgg cggccgctca cccccgagca ggaggcctac gccctcgacg
     5581 acgtgctcca tctcatcccg ctccgggagc gcctgctgga ggccctccac gcgatcgggc
     5641 gggcgcagtg ggtggaggag gaatgcgccg ggctggccgc gctggtcgtg cccgagaagc
     5701 cggcggatcc cgacgcctac atgaagatca agggcgcccg cgagctggac gcgcgcgggc
     5761 tcgcggtgct gcgcgagctg tacggcgcgc gcgagatcct ggcgctgcgg ctcgatcgcc
     5821 cgccgttcat gatcgtgggc aacgagagcc tcgtggtgct cgcggcccgc cggcccctgg
     5881 acgccgaggg catcctggcg gtgccgggct gcacgccgcc ggtggtgcgc cgcgccgggc
     5941 ccgccatcct cgacgcgatc gcgcgcgcgc tcgccctgcc cgacgcggag ctgcccgcct
     6001 accgtccggc cccgcggccg cacgtgcccg cggctcagcg gcggcgggga gaagccctgc
     6061 gcgaatggcg cgccaaggcc tcgaaggaga tcggcctcga gccgggcgtg ctctttcccc
     6121 agcgcctcat cgaccggctc gccgcggcgc cgcccgccga tcttgccgcg ttgcgacagg
     6181 tcgagggcgt gcgcgactgg cgcgtcggcc tcttcggcgc ggacctgctg aaggcgctcg
     6241 cggtcgcctg acgctccggc tcccgaccgc ttgccctcgg taccggttgg tggtaccgct
     6301 gaggccagga tctggatcgg caggatctgc actggaaggg agggtcccca tggcctccga
     6361 ttcccgctac tttcgcgaag gtatcatcgc cggcctgatc ggggccgcgc tcgtggccgt
     6421 ctggttcctc atctacgacg cggcgatcgg gcggccgttc cgcacccccg cgctgctcgg
     6481 cgccgccacc ttccggggtg tgacggatcc ggcggcggtg cccaccgcgg ccagcctggt
     6541 cgtgcagtac acggtgctgc acggcgtggt gttcgccctg atcggcgtac tcatcgcgta
     6601 cctgatcgcc tcggcccagc gcgagccgag ccgcctgctg accctgttca tcgcgctgat
     6661 gtgcttcgag gtgttcttcc tcgcggtgct gacgtggctc gcgcacccgg tgctcggcga
     6721 gctcgcctgg tgggcggtcc tcgtcggcaa cgcgctggcc gccggcggca tgctcatcta
     6781 cttcttcgtc ggccatcgcg cgctcgggcg cgccctgctg gggccgctct ggacgcgcgt
     6841 ggtgcgcgag ggcatcgtgg ccgggttgct gggcgcgggc gcggtcgcgc tctggttcct
     6901 cgcctacgac gcggccgccg gggcgcccct gcgcactccg gccctgctgg gcgccgcgct
     6961 cttccacggt ctgcgcgagc cgggcgcgct ggtgatcacg atgccgctcg tgctgcagta
     7021 cacggtggtc cacggcgccg tcttcgtgct cttcggcctc gccgcggcgg ggatgctggc
     7081 tctggccgac cgcgagccgc gcctgctctt cgccttcgtc atgctgctct gctgcttcga
     7141 ggtcttcttc gcggcgatgg tggcgatcct cgccgagtgg ctgctcgagg cggttccctg
     7201 gtggaccatt ctcgcgggca acctcctcgc tgcgctcgtg atgatgggct acttcctccg
     7261 cggccaccgg gtcacctggc acgagttcat ccacgcccgc cgctagtgtg gggtcaggtc
     7321 ttacattccg acattttcgc cgggaccgag tcagccctca cgctccaaaa tgtcggaatg
     7381 taagacctga ccccgatcgg gtagacttct gccacgacgc ggtacatgag actggcaagg
     7441 aggcgggcat cgtggcggac ggagcgctca aggggcgggt ggcggtggtg accggcggca
     7501 acggagggat cggcctcggc atggcccgcg ggctcgccgg cgccggcgcg gccgtcgtcg
     7561 tctcgggccg caacgcggag aagagccggg gcgcggtcgc cgagctcacc aagctcgggg
     7621 ccgccgcggt ggcgatcgag gccgacgtgg ccgacgaggg ggcggtcaac gcgctggtgc
     7681 ggg
//
LOCUS       DASUFA010000040         5508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_924927, whole genome shotgun sequence.
ACCESSION   DASUFA010000040 DASUFA010000000
VERSION     DASUFA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924927"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="phoU"
                     /locus_tag="VFX87_01455"
     CDS             <1..436
                     /gene="phoU"
                     /locus_tag="VFX87_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX4993598.1"
                     /translation="RDLRFITLALKVSTDLERIGDLCVNIAERTLELNEEPQLKPYID
                     LPRMADKAQRMVKESLDAFVAGDTSLARQVCGEDAEVDALKEQIFRELLTFMMEDPRT
                     VSRAIRVILISRFLERVADHATNIAEMVIYLVEGKMVRHTLA"
     gene            462..1493
                     /locus_tag="VFX87_01460"
     CDS             462..1493
                     /locus_tag="VFX87_01460"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX4993599.1"
                     /translation="MSPERHYHLQSEDANRRIDALLEQLRVPESTWPLYADLLTTALK
                     MYEDGAGVADLRIANAAFKEIRYGFKVFAPWRTVPKITVFGSARTPRAHPISEQAYAF
                     SKRITGAGWMVITGAGGGVMSSAQEGAGGERSFGLNIRLPFEQEANPWIADDPKLVSF
                     KYFFTRKLFLVREAHAMAFLPGGFGTADEAFEALTLIQTGKSPVLPLVMLDEPGGTYW
                     KTFDEFIRREMVGRGTVSPNDPELYRITDDVEQAAAEVENFYKLYHSQRYVRDALVLR
                     IRRPIALAALADLNARFADILSAPAAQAKGPIPGEGDELPELPRLVLPFNRSGFSRLR
                     SLLDFVNAS"
     gene            complement(1551..1739)
                     /locus_tag="VFX87_01465"
     CDS             complement(1551..1739)
                     /locus_tag="VFX87_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993600.1"
                     /translation="MKPKIPWLPHEVQPGQPTETCPRCGAKKLMPWTLRRDPQRVTLL
                     RTWVCTACQTTEERDEAE"
     gene            1768..2652
                     /locus_tag="VFX87_01470"
     CDS             1768..2652
                     /locus_tag="VFX87_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4993601.1"
                     /translation="MPTAYLVLLVSNLVFATGYSVARIVLEDIGPATLAMVRLTIGSL
                     ILVPWAWRGMAAAKLSREDHWRLALTGVVGFTLAFALGNWGLAHSTASNAALLITVEP
                     ASLILLSPLVLGERLSRPEGLGAALAILGAAVIVVNGIPGVTLALAPHWRGDLVLILS
                     GVAYAAYSLIARPVLLRHPALVVTAYSLVWGAAAMLPLAALEWATAPPPAWTPRAMAG
                     ALYLGVVMTALGYAVWNWCLERVETPRVAIFLNIQPLGGALLGVWWLGERLTAFTVAG
                     GLLILAGLHLTMKARRRG"
     gene            2662..4386
                     /locus_tag="VFX87_01475"
     CDS             2662..4386
                     /locus_tag="VFX87_01475"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX4993602.1"
                     /translation="MRRVRVGLAQVNPTVGAIEANARLVMAGMDRARSLGCDIIAFPE
                     LVLTGYPPEDLLFKPAFIEANLRALADVARASRGLTAVVGFVDKRDDIFNAAAILHDG
                     AQAGIYHKQYLPNYGVFDENRYFQSGTEAPVFTLGETVIAANICEDIWYPTGPTTLQA
                     LGGAELVLSINASPYHAGKGRFREKMLATRAADDLVCLAFVNTVGGQDELIFDGQSFI
                     FNEKGECVARGKPFEEDFVVADLDLDEVFRVRLHDSRRRKEKLRATATQSRRVALSPR
                     PTPERPKLPPHEVPVLEPVEEIYQALVLGTRDYVTKNGFSHVALGLSGGIDSALVVAI
                     ACDALGAANVTGVTMPSQYSSAGTRSDAARLARSLGIEFLRLPITPVLATYKRALAKP
                     FKGLKEDVTEENLQARIRGNYLMALSNKFGWLILTTGNKSEIGVGYSTLYGDMAGGFA
                     VIKDVPKTLVYQVAEHVNRKAGRDVIPRSIFDRPPSAELRPDQIDQDTLPPYAVLDAI
                     LEAYVEGDQGVADIVARGFDATTVKRVIAMVDRNEYKRRQGPIGIKITPRAFGKDWRL
                     PIVNRFRE"
     gene            4402..4728
                     /locus_tag="VFX87_01480"
     CDS             4402..4728
                     /locus_tag="VFX87_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEX4993603.1"
                     /translation="MLHSIALFALAGLAEIGGGYLMWLWWREGRPWPVGLVGAAVLVL
                     YGLIPTYQAAHFGRVYAAYGGVFVVLSLLWGWGADRVIPDRYDLAGAALCLLGVAIIM
                     YSPRGA"
     gene            4886..5407
                     /locus_tag="VFX87_01485"
     CDS             4886..5407
                     /locus_tag="VFX87_01485"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX4993604.1"
                     /translation="MAIGGRIPPADHLRRPWRVHGLAAGEGLALHDVWEVALPLAPGV
                     TLAAWLEAFRAEPRPLASRALFGLRAALGRVLRLDRGSAGLVPAYQDADEQLFRIANR
                     TVTAFLHVSVADRRPRFAVYVRPKGRLGQVYMQLIEPFRRWVVYPAMLAAGARAAARL
                     NRDQDRPSPCPLP"
BASE COUNT          774 a         1972 c         1913 g          849 t
ORIGIN      
        1 gcgcgacctg cgcttcatca cgctcgcgct gaaggtctcg accgacctgg agcgcatcgg
       61 cgatctgtgc gtcaacatcg ccgagcgcac gctcgagctc aacgaggagc cgcagctcaa
      121 gccctacatc gacctgccgc ggatggcgga caaggcccag cgcatggtca aggagagcct
      181 cgacgccttc gtggccggcg acacctcgct ggcccgtcag gtgtgcgggg aggacgccga
      241 ggtggacgcc ctcaaggagc agattttccg ggagctcctg accttcatga tggaggaccc
      301 gcgcacggtc tcccgcgcga tccgggtgat cctgatctcc cggttcctgg agcgcgtggc
      361 cgaccacgcc accaacatcg cggagatggt catctacctg gtcgagggga agatggtccg
      421 gcatacgctg gcctgaatgg gacgccgtgt atactgctga tgtgagtccg gaacggcatt
      481 accatctgca gtccgaggac gccaaccggc gaatcgacgc gctgctcgag cagctgcggg
      541 tgcccgagtc cacctggccg ctctacgcgg acctcctcac caccgcgctc aagatgtacg
      601 aggacggcgc gggggtggcc gatctccgga tcgccaacgc ggcgttcaag gagatccgct
      661 acggcttcaa ggtcttcgcg ccctggcgga ccgtgcccaa gatcaccgtg ttcggctcgg
      721 cccggacgcc gcgcgcccac ccgatctccg agcaggccta cgccttcagc aagcgcatca
      781 ccggcgcggg atggatggtc atcaccggcg cgggcggcgg cgtcatgagc tcggcccagg
      841 aaggcgcggg cggcgaacgc tcgttcgggc tcaacatccg gctgccgttc gagcaggaag
      901 ccaatccctg gatcgcggac gacccgaagc tggtctcgtt caagtacttc ttcacccgca
      961 agctcttcct ggtacgcgag gcccacgcca tggccttcct gcccggcggc ttcggcactg
     1021 ccgacgaggc gttcgaggcc ctgaccctga tccagaccgg gaagtcgccg gtgctgccgc
     1081 tcgtgatgct ggacgagccg ggcgggacct actggaagac cttcgacgag ttcatccggc
     1141 gggagatggt ggggcggggc acggtgtcgc cgaacgaccc cgagctctac cgcatcaccg
     1201 acgacgtgga gcaggccgcc gcggaggtcg agaacttcta caagctctac cactcccagc
     1261 gctacgtgcg cgacgcgctc gtgctccgca tccgccggcc gatcgccctc gcggcgctcg
     1321 ccgatctgaa cgcgcgcttc gccgacatcc tgagcgcgcc cgccgcgcag gcgaaggggc
     1381 cgataccggg ggagggggac gagctgccgg agctgccgag gctggtgctg cccttcaacc
     1441 gctcgggctt ctcccgcctc cgcagcctgc tggacttcgt gaacgccagc tagcgctatg
     1501 cctctcccct ccggggagag ggcagggtga ggggcggcag ggtgacgcgg ctactccgcc
     1561 tcgtcccgct cctcggtcgt ctggcaggcg gtgcagaccc acgtgcggag gagggtgacg
     1621 cgctggggat cacgcctcag cgtccacggc atcagcttct tcgcgccgca gcggggacac
     1681 gtctcggtcg gctgacccgg ctgcacctca tgcgggagcc acgggatctt gggcttcatg
     1741 cgcgggatta tacgctaagc tcggccggtg ccgaccgcct acctggtcct cctcgtctcc
     1801 aacttggtct tcgccaccgg ctactcggtc gcccggatcg tcctcgagga catcggcccg
     1861 gccacgctgg ccatggtgcg cctcacgatc ggctcgctga tcctcgtgcc gtgggcctgg
     1921 cgcggcatgg cggcggccaa gctgtcccgc gaggaccact ggcgcctcgc gctcaccggc
     1981 gtcgtgggct tcacgctggc cttcgcgctc ggcaactggg gcctggccca ctccaccgcc
     2041 agcaacgcgg cgctgctgat cacggtggag cccgcctccc tgatcctcct ctcgccgctg
     2101 gtgctcggcg agcgcctctc ccggccggaa gggctgggcg ccgcgctggc catcctgggc
     2161 gcggcggtca tcgtcgtgaa cggcatcccc ggcgtgacgc tggccctggc gcctcactgg
     2221 cgcggggacc tggtgctgat cctctccggc gtcgcctacg ccgcgtactc gctgatcgcc
     2281 cggcccgtgc ttctccgcca tcccgcgctc gtggtgaccg cgtactcgct cgtctggggc
     2341 gccgccgcga tgctgccgct cgcggccctc gagtgggcga ccgctccgcc gcccgcgtgg
     2401 acgccgaggg cgatggcggg cgcgctctac ctgggcgtcg tgatgaccgc gctgggctac
     2461 gcagtgtgga actggtgtct ggagcgggtg gagacacccc gcgtggccat cttcctcaac
     2521 atccagccgc tgggcggcgc cctgctcggg gtctggtggc tcggcgagcg gctcaccgcc
     2581 ttcacggtgg ccggcgggct gctgatcctg gccgggctgc acctgacgat gaaagcccgc
     2641 cggagagggt agaataccgg catgcggcgg gtgcgggtgg ggctggccca ggtgaatccg
     2701 acggtgggcg ccatcgaggc caatgcgcgg ctcgtgatgg cgggcatgga ccgggcccgt
     2761 tcgctgggct gcgacatcat cgcgttcccg gagctggtgc tgaccgggta cccgccggag
     2821 gacctgctct tcaagcccgc cttcatcgag gcgaacctcc gggcgctggc cgacgtggcc
     2881 cgggcgagcc gcgggctcac cgccgtggtg ggcttcgtgg acaagcgcga cgacatcttc
     2941 aacgccgcgg ccatcctgca cgacggcgcg caggccggta tctatcacaa gcagtacctg
     3001 cccaactacg gcgtcttcga cgagaaccgc tacttccagt cggggacgga agcgccggtc
     3061 ttcacgctcg gcgagacggt catcgcggcg aacatctgcg aggacatctg gtacccgacc
     3121 ggccccacca cgctgcaggc cctgggcggc gccgagctgg tgctgagcat caacgcctcg
     3181 ccgtaccacg cgggcaaggg gcgcttccgc gagaagatgc tggccacccg ggccgccgac
     3241 gacctcgtat gcctggcctt cgtcaacacg gtgggcgggc aggacgagct gatcttcgac
     3301 ggccagtcct tcatcttcaa cgagaagggc gagtgcgtgg cgcgggggaa gcccttcgag
     3361 gaggacttcg tcgtcgccga tctcgatctc gacgaggtct tccgggtgcg gctgcacgac
     3421 tcgcgacggc gcaaggagaa gctgcgcgcc accgcgaccc agtcgcgccg cgtcgcgctg
     3481 tccccgcggc ccacgccgga gcgaccgaag ctgccgccgc acgaggtgcc ggtgctggag
     3541 ccggtggagg agatctacca ggccctcgtc ctgggcaccc gcgactacgt caccaagaac
     3601 gggttcagcc acgtggcgct ggggctctcg ggcggcatcg actcggcgct ggtggtggcc
     3661 atcgcctgcg acgcgctggg cgccgccaac gtcaccggcg tgaccatgcc gtcgcagtat
     3721 tcctcggcgg gcacccggag cgacgcggcg cggctggccc gcagcctcgg catcgagttc
     3781 ctgcggctgc ccatcacgcc ggtgctggcc acctacaagc gcgccctcgc caagcccttc
     3841 aaggggctca aggaggacgt gaccgaggag aacctgcagg cccgcatccg gggcaactac
     3901 ctcatggcgc tctccaacaa gttcgggtgg ctcatcctca ccaccggcaa caagagcgag
     3961 atcggggtgg gctactccac gctctacggc gacatggccg ggggcttcgc ggtgatcaag
     4021 gacgtgccga agaccctcgt ttaccaggtg gccgagcacg tcaaccggaa ggcagggcgc
     4081 gacgtcatcc cgcgatccat cttcgaccgc ccgccgtccg ccgagctgcg ccccgatcag
     4141 atcgaccagg acacgctgcc gccctacgcg gtgctggacg cgatcctgga ggcctacgtg
     4201 gagggcgacc agggcgtggc cgacatcgtg gcacgcggct tcgacgcgac cacggtcaag
     4261 cgcgtgatcg ccatggtgga ccgcaacgag tacaagcggc gccagggccc catcggcatc
     4321 aagatcacgc cgcgggcctt cggcaaggac tggcgcctgc ccatcgtcaa ccgcttccgc
     4381 gagtagcgcc ggctgcgccc cctgctccat tcgatcgcac tgttcgcgct ggccggtctc
     4441 gccgagatcg gcggcggcta cctcatgtgg ctctggtggc gcgagggccg gccgtggccg
     4501 gtggggctgg tgggcgccgc cgtcctcgtc ctgtacggcc tgatcccgac ctaccaggcg
     4561 gcccacttcg ggcgcgtcta cgccgcctac ggcggagtct tcgtggtgct ctcgctgctc
     4621 tggggctggg gggcggatcg cgtcattccc gatcgctacg acctggccgg cgccgcgctc
     4681 tgcctgctgg gcgtcgcgat catcatgtac tcgccgcgcg gcgcctagcc cgcgctgtgc
     4741 ctgaacccat gcacccccca ccccgaccct ctccctcggc gagggagagg gggcgtttcg
     4801 atcccctcgc ccccggaggg gagagggaca gggtgagggg gtgcgttgga cgcggagcac
     4861 gcgttcgtga atggagcaag ctagcatggc gatcggcggc cgcatccccc ccgcggatca
     4921 cctgcgccgg ccgtggcgcg tccatggcct ggccgccggg gaagggctcg ccctgcacga
     4981 cgtctgggag gtcgcgcttc cgctggcccc cggcgtgacc ctggccgcct ggctcgaggc
     5041 ctttcgcgcg gagccgcggc cgctcgcgag ccgcgcgctc tttgggttgc gggcggccct
     5101 cggccgcgtg ctgcggctcg atcgcggcag cgccgggctc gtgcccgcgt accaggacgc
     5161 cgacgagcag ctcttccgca tcgccaaccg cacggtcaca gcattcctgc acgtctcggt
     5221 ggcggaccga agaccccgct tcgcggtcta cgtgcgcccg aagggccgcc tcgggcaggt
     5281 ctacatgcag ctcatcgagc ccttccgccg gtgggtggtc tacccggcca tgctcgcggc
     5341 gggtgcgcgc gcggccgcac gcctgaaccg ggaccaagac cgcccctcac cctgccctct
     5401 cccctgaggg gagagggggg gagaagggtc agctcgcggg gagggcgtcg agcgactgga
     5461 gcgtggcgag gcgccggtag atgccgtcgc cccgggccat cagctcct
//
LOCUS       DASUFA010000041        14001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1039577, whole genome shotgun sequence.
ACCESSION   DASUFA010000041 DASUFA010000000
VERSION     DASUFA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039577"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3428)
                     /locus_tag="VFX87_01490"
     CDS             complement(<1..3428)
                     /locus_tag="VFX87_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4993605.1"
                     /translation="MNFLSPFALLMATLSVPLLLLYFLKVRRRQMRVSSLLLWAPALR
                     DREASTFFQRLQRDPLLILQILALLALTAALARPAVNVMGQGAKRVAIVMDISGSMKA
                     TDVSPSRFVQAQRAALGLLGRLGEGAEVMVVEAGVQPRVLVPFTREHDRVSSAIRSME
                     AHDLPNRLQEGLRTARALVGPDPRAEIHVFTDGAHPDAVKGQGDDVRVRWTGTGLRSH
                     NVGITNFAVRRSYFGSYNSQAFFSVGNFSGERQTLSLKLTLDDEVLTQRTLTLDPQVR
                     RAITVPFSEQRGGVLKLKLDVSDDLDADNTAYAVMPPPRMISALLVSPGNLFLEKVLK
                     TDPQVQLEVRKPDAYQGGMEGFDVVVVDSVSPQKLGNGRFVLVNTVPADVPLEVLGRL
                     DNPTIMDWDRTHPIMRQIDFAKVTMEDAMRVRPLAAGKTLVEAVGGPLIYALEERDRK
                     ALFFGFDLFRSDFPLRVAFPLMLSKGLRWLHPAGLDQASLQLQAGQPILLPVEHGVTT
                     ATVRTPSGRSVRAQVNRGMASFTETDEVGIYAVVTGRGETRVAVNLMNGDESDLTPQP
                     LPTFVEGARPESPPVLIVRELWPFFVALALLLFALEGFLYWRRQTGGRLGLPRGLGDR
                     WALGLRCALLAMLLFSLTKPTIPRWADRLNVTFLLDMSDSVSLAARESAYRFAAQSVA
                     NMQPGDQAGVVVFGEEAMVDQPLRATAKLERPQAQVGGRGTNLAQALQLGLATAPPGQ
                     ANRFVLLTDGRQNQGNALAVAQAAKDAGADIYYVPAPLTFPQEVVVESMVLPQEVKFG
                     EPFQAKVVAWSQAETQGRLSLYRNAEFLGSQVVRLSPGKNVFTYRQSLEQSGIHVYQA
                     ALDVDGDTIEENNRAVGTLVVRGRPQVLLAEKDRNQAQALAAALRSQHVDVTVVEADK
                     IPKDMAGLQKYDGLILSNVSSLKMTKKQMENIRDYVREQGGGLVMLGGEESFGLGGYY
                     RTPIEEALPVTMEVKQKIEIPSLAVVLSIDRSGSMAMTTDEKVTKLDIAKEAAHLVVD
                     LLDERNEVGVMSWDTEFIWDAPLKPARDKQGIHHAISTIKAGGGTDGYPALKEAYQVL
                     FDRPALLKHVIFLSDGQMTRGDFSGLLRRMAKDKITVSTVAIGKDADVPLMVDVA"
     gene            complement(3425..4318)
                     /locus_tag="VFX87_01495"
     CDS             complement(3425..4318)
                     /locus_tag="VFX87_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4993606.1"
                     /translation="MATARAGHELLSTDFLTQLERLSLLSRRSFRGRVKGERRSPRKG
                     ISVEFSDYRPYGVGDDLRYVDWNVYARLDRLYLKLFVDEEDLCLHLLVDASASMGFGE
                     PSKLDYSARLAGALGFVGLVGLERVGVGVVRDRMAEGFNPARGRSQAIPLMDFLGRLR
                     AGGTTSLNDGLAAYALRSREAGLAVLVSDLMDPGGYERGLKALLERRFDVHVVHVLAA
                     DEMNPAFGGDLRLLDAETDEERDLTLDGEALRGYHERLRAFLDRAEQFCRSNEITYHR
                     VVTETPVEEFMLRQLKGLLLA"
     gene            complement(4323..5807)
                     /locus_tag="VFX87_01500"
     CDS             complement(4323..5807)
                     /locus_tag="VFX87_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993607.1"
                     /translation="MPGPTVIERLVADVARQIRLRRAEFYGLRGLLWGSVAAVVPLVL
                     KESLGIWSYAGAAILLLAGAGAGVLWGFLLKLPQAEVARLADRGYGLQDRVSTALEWA
                     DRADRTPIVEALVADAAARADQLGARRIIPRRMPREAKLVPVPLVLGLVLAAAPPIPL
                     PQGGLPNFSVSRDEEEEKLKERGGDLQQSERRQATRRDPVQRAEVQERTLTPRMGGGG
                     QSQPGDLAAVFKDTSLAGRAPDFNSFLKKGDDRLRMLEQTDRLPDLQSDFTQNQTRMV
                     FQKAKSLRGGLDPNKKVSPEKLRELLQEMERLGRKNQPGGGQSDWSGDVYEGMEALEG
                     GQSDKAMEAMERALNKMRSQEERGRDGKSLKGGRESDRRGNRRGNEKGQGQGGPGDEG
                     DFPEGEGLLPGRGKSGSPKGDPTARLRGNPFDVGVEGEARSGRKQGIDTNMVGRGSQM
                     PSRLQYMGVLGQYRKMMEESIAREQVPRDFQSQVKEYFQALDEK"
     gene            complement(5824..6861)
                     /locus_tag="VFX87_01505"
     CDS             complement(5824..6861)
                     /locus_tag="VFX87_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4993608.1"
                     /translation="MAQPPVSSQPQEQRVQEFVSAYQALRAEVGKVIVGHNEVVSEVL
                     VGLFAGGHVLLEGVPGLGKTLLIKTLAESLELSFSRIQFTPDLMPGDIIGTNIIVEDA
                     QARKHFQFQRGPIFGHILLADEVNRATPKTQSALLEGMQEGAVTVSGHSHPLPQPFFV
                     LATQNPIEMEGTYPLPEAQLDRFLFKLRVRYPALEELTEIIDRTTHVRQVEVRRVLTG
                     AQVLTMRELVREIPIATHVRDLASYIVLATHPQWERAPESTRRFIRYGASPRGAQALV
                     LGAKVRAIAEGRFNVSVEDLRATALPALRHRLILNFEGEAEGVDVDDLIRQTVDAAET
                     MTGSEKEVFLR"
     gene            complement(7079..7678)
                     /locus_tag="VFX87_01510"
     CDS             complement(7079..7678)
                     /locus_tag="VFX87_01510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4993609.1"
                     /translation="MPATGHWPPIAALERLPGPIILFPAGIRKVKRPAFPLEPLERLR
                     DRYPTLELVYVGPIIDAAEGEALLGLLSGYPWARYVGAVPHDRMPALFELADVVLNCS
                     LSEGGMANSVLEALSLGRAVLASDIAGNRSLVDDAITGFLFGSAEELAAKADRLLQDP
                     DLRARLGVAGRERVNARFGADRETDGYLAVYARLVPGFN"
     gene            complement(7709..8131)
                     /locus_tag="VFX87_01515"
                     /pseudo
     CDS             complement(7709..8131)
                     /locus_tag="VFX87_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            8265..8528
                     /locus_tag="VFX87_01520"
     CDS             8265..8528
                     /locus_tag="VFX87_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX4993610.1"
                     /translation="MDGASREIGCPECVELLADYIDGSLPPDQTAQLEWHLDGCPPCV
                     AFVNTYKGTVNAARRLREHPETHLPAELRQKLIAFLKRSDASR"
     gene            complement(8624..9691)
                     /gene="selD"
                     /locus_tag="VFX87_01525"
     CDS             complement(8624..9691)
                     /gene="selD"
                     /locus_tag="VFX87_01525"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(9617..9619),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX4993611.1"
                     /translation="MDPKLIDPKTGKEIRLTAYAACAGUASKMGPGDLQDVLGDLRHE
                     EHPDLLVGLGRSDDAAVYRVTDDVAIVETVDFFPPIVDDPYLFGAIAAANSMSDVYAM
                     GGQVLFALNVAGFPRELPKDIIAAVFRGGADKVREAGGVIAGGHTVVDAEPKYGLCVT
                     GRVDPRRMLIKGGLRPGQRVFLSKPLGTGVIATAAKNDKCERAVLDGAVQSMLRLNRV
                     AAQVAQEAGATGATDITGFGFLGHASEMVAASAAGLIVRSRALPLLPGALVLAEQGSF
                     SGGMGRNRRHLEDDFGRLRIDPAVPEPLIKLLCESETSGGLLFAVDPARAATVHEAFA
                     RAAEPVWEIGEVTAEPHLALI"
     gene            complement(9738..10631)
                     /locus_tag="VFX87_01530"
     CDS             complement(9738..10631)
                     /locus_tag="VFX87_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HEX4993612.1"
                     /translation="MDLRQLEIFVKVAELGSFSKAADALFLTQPTVSEHIRSLEDELG
                     VRLLDRLGRGAAVTKGGALLQGYAQRLLALSREARQAIESFQGRMSGELLVGASTIPG
                     EYILPALIGRFKEKFPDIAITLLIGGSQEVTEWVAEGRAEVGVVGARSGHRGIDFREL
                     LPDDIVLIVSAAHPWQGRKQVTLEELRAEPLLLRERGSGTRAAFEAALTQAGSDVAAL
                     RVVGEMGSTQAIKQAVKANVGVSLVSRRAVEEECRAGSLWCLRVKDLKITRAFYLATH
                     RERSRSPLAEAFRAFVEAEAV"
     gene            complement(10762..10983)
                     /locus_tag="VFX87_01535"
     CDS             complement(10762..10983)
                     /locus_tag="VFX87_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX4993613.1"
                     /translation="MPIYEYACLACGAEFEKLVQRPGPVVCASCESERVSRRLSIVGV
                     KTGGRMSDASPAPMGGGGGGCCGGGCGCR"
     gene            complement(11020..11223)
                     /locus_tag="VFX87_01540"
     CDS             complement(11020..11223)
                     /locus_tag="VFX87_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007321455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX4993614.1"
                     /translation="MAEQKKKETRPTKPAEGGDDGGAGSAELSKKGKKIKEELDNLLD
                     EIDDILEENAEEFVKSYVQRGGQ"
     gene            complement(11271..11822)
                     /gene="dcd"
                     /locus_tag="VFX87_01545"
     CDS             complement(11271..11822)
                     /gene="dcd"
                     /locus_tag="VFX87_01545"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX4993615.1"
                     /translation="MIKSDRWIRRMAVEKGMIKPFEDRQVRKGGISYGLSSYGYDIRI
                     ADEFKIFTNINTTIVDPKSFDPRSFVDYQGEVAVIPPNSFALGRSVEYFKIPRNVLTI
                     CVGKSTYARCGIITNVTPFEPEWEGYVTLEISNTTPLPARIYANEGIAQVLFFESDEA
                     CETSYADRQGKYQGQQGIVLPSV"
     gene            complement(11866..12774)
                     /gene="thrB"
                     /locus_tag="VFX87_01550"
     CDS             complement(11866..12774)
                     /gene="thrB"
                     /locus_tag="VFX87_01550"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX4993616.1"
                     /translation="MRVRVRVPATSANLGPGFDTLGLALGLHNEVVLEEADGISVSVE
                     GEGAGRLDDDGKNVVARGVALGFEVAGRPFRGGRLRCVNRIPLSRGLGSSAAAWVGGL
                     VAANALLGEPLDRDALVTAAARAEGHPDNVAAAVLGGLTVSCADGARVAAVSLPVPRE
                     IVWVVLLPGTESSTREARAVLPDSVPRADAVFNVQRVSLLLAALGAGRPDLLDLAMQD
                     RLHQPYRQRLFPWLEAVAAAGRRAGALGCVLSGAGPSMLAAARGGGDEVARAMERALR
                     EAGIEGRALSLPVDTEGATWERLPET"
     gene            12831..13976
                     /gene="pdhA"
                     /locus_tag="VFX87_01555"
     CDS             12831..13976
                     /gene="pdhA"
                     /locus_tag="VFX87_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX4993617.1"
                     /translation="MPKTIIEPQFTIEHLSILDSDGNLDSALDPAIAPDDLKRLYRAM
                     LLARRFDERMLRLQRQGRIGTFAPIKGQEAAQLGSVAALRRTDWMVPSFRETASMLWR
                     GWPMEKILLFFSGYLEGGRPEADQRDLPICIPVATQLPHAVGLAYAAQYRGDDAVVMV
                     YCGDGATSEGDFHEALNFAGVWHVPVVFIVQNNQWAISIPLKKQTHSRTIAQKALAYG
                     LPGLQVDGNDVLAVYAASLEAVERARRGDGPTLIECVTYRLGVHTTSDDPTKYRSTEE
                     VEAWERKEPLTRFRGYLQQRSLLEPGLEEAVDAEIARGVAAFEAVGPPDPLGMFDHVY
                     GNPPAALAAQRAEMAARLGGAPQPAAEPPPGPAPSPPMRGQRRSWPS"
BASE COUNT         2098 a         5072 c         4768 g         2063 t
ORIGIN      
        1 gccacgtcca ccatgagggg cacgtccgcg tccttgccga tggccacggt ggagaccgtg
       61 atcttgtcct tggccatccg tcgcagaagg cccgagaagt cgccgcgggt catctgcccg
      121 tccgacagga agatgacgtg cttcagcagc gccggccggt cgaagaggac ctggtaggcc
      181 tccttcagcg ccgggtagcc gtcggtgccg ccgcccgcct tgatcgtgga gatcgcgtgg
      241 tggatgccct gcttgtcccg ggccggcttg aggggggcgt cccagatgaa ctcggtgtcc
      301 cagctcatga ccccgacctc gttgcgctcg tcgaggagat cgaccaccag gtgggcggcc
      361 tccttggcga tgtcgagctt ggtcaccttc tcgtcggtgg tcatggccat cgagcccgag
      421 cggtcgatcg agagcaccac cgccaggctc gggatctcga tcttctgctt cacctccatc
      481 gtcaccggga gcgcttcctc gatcggggtg cggtagtagc cgccgaggcc gaagctctcc
      541 tcgccgccca gcatcacgag cccgccgccc tgctcgcgca cgtagtcgcg gatgttctcc
      601 atctgcttct tcgtcatctt gagcgacgac acgttggaca ggatgaggcc gtcgtacttc
      661 tgcagccccg ccatgtcctt cgggatcttg tccgcctcca ccaccgtcac gtccacgtgc
      721 tgcgagcgca gggccgcggc caaggcctgg gcctggttgc ggtccttctc ggcgagcagc
      781 acctgcggcc gcccgcgcac caccagggtg cccaccgcac ggttgttctc ctcgatggtg
      841 tcgccgtcca cgtcgagcgc ggcctggtac acgtggatcc cgctctgctc gagcgactgc
      901 cggtacgtga acacgttctt gcccggggag aggcgcacca cctgcgagcc gaggaactcg
      961 gcgttgcggt agagcgagag ccgcccctgc gtctccgcct ggctccaggc gaccaccttg
     1021 gcctggaacg gctcgccgaa cttcacctcc tgcggcagca ccatggactc cacgacgact
     1081 tcctgcggga aggtcagtgg ggccgggacg tagtagatgt cggcgcccgc gtccttggcc
     1141 gcctgggcga ccgcgagcgc gttgccctgg ttctgccgcc cgtccgtgag cagcacgaac
     1201 cggttggcct gcccgggtgg cgcggtggcc agcccgagct ggagcgcctg ggccaggttc
     1261 gtcccgcgcc cgcccacctg ggcctggggc cgctcgagct tggcggtcgc gcgcagcggc
     1321 tggtccacca tggcttcctc gccgaacacc accacgcccg cctgatcgcc cggctgcatg
     1381 ttggccactg actgcgcggc gaatcggtag gcgctctcgc gcgcggccag gctcacactg
     1441 tccgacatgt ccagcaggaa ggtcacgttg agccggtcgg cccagcgcgg gatcgtgggc
     1501 ttggtcagcg agaacagcag catcgcgagg agcgcgcagc gcagaccgag ggcccagcgg
     1561 tcgcccagcc cacggggcag cccgaggcgc cccccggtct ggcgacgcca gtagaggaag
     1621 ccctccaggg cgaagagcag cagggccagg gccacgaaga agggccagag ctcgcggacg
     1681 atgagcaccg gcggcgactc cggccgcgcg ccctccacga aagtggggag cggctgtgga
     1741 gtcaggtccg actcgtcgcc gttcatcagg ttcacggcca cgcgcgtctc gccgcgaccg
     1801 gtcaccaccg cgtagatacc gacctcgtcg gtctcggtga agctcgccat cccgcggttg
     1861 acctgcgccc gcacgctgcg cccggagggg gtgcgcacgg tggcggtggt caccccgtgc
     1921 tccaccggca gcaggatcgg ctgacccgcc tggagctgca ggctcgcctg gtcgagccca
     1981 gccgggtgca gccagcggag acccttggag agcatgagcg gaaaggccac ccgtagcggg
     2041 aagtcgctgc ggaagagatc gaagccgaag aagagggcct tgcggtcgcg ctcttccagg
     2101 gcgtagatca gcggcccgcc caccgcctcc accagcgtct tcccggcggc gagcggccgg
     2161 acgcgcatcg cgtcctccat cgtcaccttg gcgaagtcga tctgccgcat gatcgggtgc
     2221 gtgcggtccc agtccatgat ggtgggattg tcgaggcgtc ccagcacctc gagcggcacg
     2281 tccgcgggca cggtgttgac gaggacgaag cggccgttgc ccagcttctg cgggctcacg
     2341 ctgtccacca ccacgacgtc gaagccctcc atgccgccct ggtaggcgtc gggcttgcgc
     2401 acctcgagct gcacctgcgg gtcggtcttc agcaccttct ccaggaacag gttgccgggg
     2461 ctgaccagca gggccgagat catccggggc ggcggcatga ccgcgtaggc ggtgttgtcg
     2521 gcgtcgagat catccgagac gtcgagcttg agcttgagca caccgccgcg ctgttcgctg
     2581 aacggcaccg tgatggcccg ccgcacctgc gggtcgaggg tgagcgtgcg ctgggtgagg
     2641 acctcgtcgt ccagggtcag cttgagcgag agggtctgcc gctcgcccga gaagttgccc
     2701 accgagaaga acgcctgcga gttgtaggac ccgaagtagc tacggcggac cgcgaaattc
     2761 gtgatgccca cgttgtgaga gcggaggccg gtgccggtcc agcgcacgcg gacgtcgtct
     2821 ccctggccct tcaccgcgtc ggggtgcgcg ccatcggtga agacgtggat ctccgcgcgc
     2881 ggatcggggc ccaccagcgc gcgcgcggtg cggaggcctt cctgcaggcg gttcgggagg
     2941 tcgtgcgcct ccatcgagcg gatcgccgag gagacccggt cgtgctcgcg ggtgaagggc
     3001 accagcactc tgggctgcac gcccgcctcg accaccatca cttcggcgcc ctcgcccagg
     3061 cggccgagca gcccgagcgc ggcccgctgc gcctgcacga agcgcgaggg cgagacgtcc
     3121 gtggccttca tggagccgga gatgtccatg acgatggcca cccgcttcgc cccctgcccc
     3181 atcacgttca ccgccggccg ggccagcgcc gcggtgaggg cgagcagggc caggatctgg
     3241 aggatgagga gcgggtcgcg ctgcagccgc tggaagaagg tggaggcctc gcggtcgcgg
     3301 agcgcgggcg cccacaggag caggctcgac acccgcatct gccgccgcct caccttcagg
     3361 aagtagagga ggaggagcgg caccgacagg gtggccatca ggagggcgaa ggggctgagg
     3421 aagttcatgc gagcaggagc cccttgagct ggcggagcat gaactcctcg accggcgtct
     3481 ccgtcaccac ccggtggtag gtgatctcgt tgctccggca gaactgctcg gcgcgatcga
     3541 gaaaggcgcg gagccgctcg tgatagccgc gcagggcctc gccgtcgagc gtgaggtcgc
     3601 gctcctcgtc ggtctccgcg tcgagcaggc gcaggtcacc gccgaaggcg ggattcatct
     3661 cgtccgcggc cagcacgtgc acgacgtgca cgtcgaagcg ccgctcgagg agggctttga
     3721 gcccgcgctc gtagccgccc gggtccatca gatccgagac gagcacggcc aggcccgcct
     3781 cgcgcgagcg cagcgcgtag gccgcgagcc cgtcgttcag cgaggtggtc ccgcccgcgc
     3841 gcaggcgacc gaggaagtcc atcagcggaa tcgcctggct ccggccgcgc gccgggttga
     3901 agccctcggc catccggtcc cgcaccacgc ccacgcccac ccgctccagc cccaccaggc
     3961 ccacgaagcc gagggcaccg gccagccgcg cggagtagtc gagcttggac ggctcgccga
     4021 agcccatgga ggcggaggca tccaccagca ggtgcaggca gagatcctcc tcgtccacga
     4081 agagcttgag gtagaggcgg tcgaggcggg cgtagacgtt ccagtccacg tagcgaagat
     4141 cgtcgccgac cccgtagggg cggtagtcgg agaactccac gctgataccc ttgcgcgggc
     4201 tgcggcgctc gcccttgacc cggccccgga aggaccggcg ggagagcagg ctcaggcgct
     4261 cgagctgggt gagaaagtcc gtcgagagca gctcgtggcc ggcacgcgcg gtggccacgg
     4321 cgctacttct catccagcgc ttggaagtac tccttcacct ggctctggaa gtcgcgcggc
     4381 acctgctcgc gggcgatgga ctcctccatc atcttgcggt actgccccag cacgcccatg
     4441 tactgcaggc gggagggcat ctgcgagccg cggccgacca tgttggtgtc gatcccttgc
     4501 ttgcgccccg aacgcgcctc tccttccaca cccacgtcga acggattgcc gcgcaggcgc
     4561 gcggtggggt cgcccttcgg ggatccgctc ttgccgcggc cgggcagcag cccctcgccc
     4621 tccgggaaat cgccctcgtc tcccgggccg ccctggccct ggcccttctc gttcccgcgc
     4681 cggttgccgc gccggtcgct ctcgcgccca cccttgagcg acttgccgtc gcgaccccgc
     4741 tcttcctggg agcgcatctt gttcagcgcc cgctccatcg cctccatcgc cttgtcgctc
     4801 tggccgccct cgagggcttc catgccttcg tagacgtcgc ccgaccagtc gctctgccca
     4861 ccgcctggct ggttcttccg tccgagccgc tccatctcct gcagcagctc gcgcagcttc
     4921 tccggcgata ccttcttgtt gggatcgagg ccgccgcgca gcgacttggc cttctggaag
     4981 accatccggg tctggttctg cgtgaagtcg ctctggagat ccggcagccg gtccgtctgc
     5041 tccagcatcc gcagccggtc gtcgcccttc ttgaggaagg agttgaaatc gggcgcgcgg
     5101 ccggccagcg aggtgtcctt gaagacggcg gccagatcgc cgggctggct ctggccgcct
     5161 ccgcccatcc gcggcgtgag cgtgcgctcc tgcacctccg cgcgctgcac cggatcgcgc
     5221 cgggtggcct gccgacgctc ggactgctgc aggtctccgc cgcgctcctt gagcttctcc
     5281 tcttcctcgt cgcgggaaac cgagaagttg ggcaggccgc cctgcggcag cggaatcggc
     5341 ggggccgccg ccagcacgag gcccagcacg agcgggacgg gaacgagctt ggcctcccgc
     5401 ggcatccggc gcgggatgat gcgccgcgcc ccgagctgat ccgcgcgggc ggccgcgtcg
     5461 gccacgagcg cctccacgat cggggtgcga tccgcacgat cggcccactc gagcgccgtg
     5521 ctcaccctgt cttgaaggcc gtagccgcgg tcggcgagcc gcgccacctc cgcctggggc
     5581 agcttcagca ggaagcccca cagcacgccg gcccccgcgc cggccagcag gaggatcgcg
     5641 gcgcccgcgt agctccagat ccccagcgac tccttgagga cgagcggcac caccgcggcc
     5701 acagagcccc acaagaggcc gcgcaggccg tagaattccg cgcgtcgcag gcggatctgc
     5761 cgggcgacgt ccgcgacgag ccgctcgatc accgtgggac cgggcatcag actctcctct
     5821 cggctatctc aggaaaacct ctttctctga accggtcatg gtctcggcgg catcgacggt
     5881 ctggcggatc aggtcgtcca cgtcgacgcc ctccgcctcg ccctcgaagt tcaggatcag
     5941 ccggtgccgg agcgcgggca gcgcggtggc ccggagatcc tccacgctga cgttgaagcg
     6001 gccctccgcg atcgcgcgaa ccttcgcgcc cagcaccagg gcctgcgctc cgcgcgggct
     6061 cgccccgtag cggatgaacc ggcgggtcga ttcgggagcc cgctcccact gcggatgagt
     6121 ggcgagcacg atgtaggagg cgagatcccg tacgtgggtg gcgatcggga tctcgcgcac
     6181 cagctctcgc atggtgagga cctgggcgcc ggtcaggaca cgccggacct cgacctgtcg
     6241 cacgtgggtg gtgcggtcga tgatctcggt gagctcctcg agagccggat agcggacccg
     6301 cagcttgaag aggaagcggt cgagctgcgc ttccggcagc gggtaggtgc cttccatctc
     6361 gatggggttc tgggtggcga gcacgaagaa cggctgcggc agcgggtgcg agtggcccga
     6421 gacggtgacc gcgccctcct gcatgccctc cagcagcgcg gactgcgtct tcggcgtggc
     6481 ccggttcacc tcgtccgcga gcaggatgtg gccgaagatg ggcccgcgct ggaactggaa
     6541 gtgcttccgc gcctgggcgt cctccacgat gatgttggtg ccgatgatgt cgcccggcat
     6601 caggtcgggc gtgaactgga tccgggagaa ggagagctcg aggctctccg cgagggtctt
     6661 gatgaggagc gtcttgccga gcccggggac gccttcgagt agcacgtgac cgcccgcgaa
     6721 gagaccgacc agcacctcgc tgacgacctc gttgtggccg acgatgacct tgccgacctc
     6781 ggcgcgcagc gcctggtagg cgctcacgaa ctcctggact cgctgctctt ggggctggct
     6841 agacacggga ggctgcgcca tttgaacggc tcctttcccg ccttggaatt gatccatcat
     6901 aggggggcgt tccagccccg tcaactacct gccccttctt tcgtcactcc gacgcgcggg
     6961 atgccggaat ttcactcagc gcgacacgtg cgacaccccc gaaacgcgcg cgaagcgccc
     7021 tcggaaaccg cgcgcgggcc gattgactcg ttgcggcagc aaagaccgtg ccgcgcgctc
     7081 agttgaagcc cggcaccagt cgcgcgtaca ccgcgaggta gccgtcggtc tcgcgatcgg
     7141 cgccgaagcg cgcgttgacg cgctcgcggc ccgccacccc gagccgggcg cggaggtcgg
     7201 gatcctgcag cagacgatcg gccttcgcgg ccagctcctc cgccgagccg aagaggaagc
     7261 cggtgatcgc gtcatcgaca agcgatcgat tacccgcgat gtccgaggcg agcaccgcgc
     7321 ggccgagcga gagcgcctcg agcaccgagt tggccatccc gccctcggag agcgagcagt
     7381 tgagcaccac gtccgccagt tcgaagagcg cgggcatgcg atcgtgcggc accgccccca
     7441 cgtaccgggc ccacggatac ccggacagca gcccgagcag ggcctcgccc tccgccgcgt
     7501 cgatgatggg ccccacgtag accagctcga gcgtcggata ccggtcgcgc aggcgctcga
     7561 gcggctccag cgggaacgcg ggccgcttca ccttccggat gcccgcgggg aacaggatga
     7621 tggggccggg caggcgctcg agcgcggcga tcggcggcca gtggccggtg gccggcatct
     7681 cgaacaccgc gctctgcggg atcaccacca ggcgggctgc cgtctcgggc agcgcctcca
     7741 tgatgcgagc ggccatcgac tcgtcgaaga cgctcaccgc ccccgccccc tccaggaccc
     7801 gccggacgat cggcgccgca tcctcgtcga agaggtcctg gttcacgtcg gtccccgtcg
     7861 ccgtcaccag caggggaacg cccgcgcgcc gtgccacccg caccgcggtg ggcccggtgc
     7921 gggtggcgtg gaacgcgtgg atgaccgccg gcccgtactg gacgatctgg tgctcgacgc
     7981 caccttcggg caccaccgag agatcccaga cgcgcagctc gacgccgcgc tgccggagac
     8041 cgcgcgcgat gcgatcgacc gtgatggcat tcccacggac cgacggcgaa gcgaagggcg
     8101 tgaggacggc cacccgagcg gccatggctg cgaggagcct acaccggccc ctcgcgacaa
     8161 ggcaattaac gcgcgggccc ggccggcaaa gacggacttg ccccgggccg gccctgtgtt
     8221 aaggtttcgc tacagttgat cgcctcatcg gcgaggggga gcagatggac ggagcgtcgc
     8281 gcgagatcgg gtgcccggag tgcgtcgagc tgctcgcgga ctacatcgac ggatcgctcc
     8341 ctccggatca gaccgcgcag ctcgaatggc acctggacgg ctgcccgccc tgcgtggcct
     8401 tcgtcaatac ctacaagggc accgtcaacg ccgcccgccg gctccgtgaa caccccgaga
     8461 cccacctccc ggccgagctc cgccagaagc tgatcgcgtt cctgaagcgc tccgacgcgt
     8521 ctcgttagct cggagggggc tgcgccccgc ttccgaacct cccccaagga caggtcgcgc
     8581 gggcaaagcc cgcgctcgga gcggctgatc ttaccgagga cgcctagatc agagcgagat
     8641 gcggctccgc ggtcacctcg ccgatctccc agaccggctc ggcggcgcgc gcgaaggcct
     8701 cgtgcacggt ggcggcgcgc gccggatcca ccgcgaagag caggccgccg gaggtctccg
     8761 actcgcagag cagcttgatc agcggctccg gcaccgccgg gtcgatacgg agccgcccga
     8821 aatcgtcctc cagatggcgt cggttccggc ccatgccgcc gctgaagcta ccctgctcgg
     8881 ccaacacgag ggcgcccggc aagagcggca gtgcccgact gcggacgatg aggcccgccg
     8941 ccgaggccgc gaccatctcg ctggcgtgcc ccaggaaccc gaacccggtg atgtcggtgg
     9001 cgccggtcgc ccccgcctcc tgcgccacct gggccgccac gcggttgagc cgcagcatgc
     9061 tctgcaccgc gccgtcgagg accgcgcgct cgcacttgtc gttcttggcc gcggtcgcga
     9121 tcaccccggt gccgagcggc ttggagagga acacccgctg gccggggcga agccccccct
     9181 tgatcagcat gcgccgcgga tccacccggc cggtcacgca gagaccgtac ttcggctccg
     9241 cgtcgacgac ggtatggccg cccgcgatga cgccgcccgc ctcgcgcacc ttgtcggcgc
     9301 cgccgcggaa gacggcggcg atgatgtcct tgggcagctc gcgcgggaag cccgccacgt
     9361 tgagcgcgaa cagcacctgc ccgcccatcg cgtacacgtc ggacatcgag ttggcggccg
     9421 cgatcgcccc gaagagatag ggatcatcca cgatgggcgg gaagaagtcc accgtctcca
     9481 cgatggccac gtcgtcggtc acgcgataga cggcggcgtc gtcgctccgg ccgagcccca
     9541 ccagcaggtc ggggtgctcc tcgtggcgca ggtccccgag cacgtcctgg agatctccag
     9601 gacccatctt cgacgctcac cccgcgcagg cggcgtacgc ggtgagccgg atctccttgc
     9661 cggtcttcgg atcgatcagc ttcgggtcca tgatggagga gcggcggccc gaggagcggg
     9721 gaccacgcga gcgacggtca gacggcctcg gcctcgacga aggcgcggaa ggcctccgcg
     9781 agcggcgagc ggctgcgctc ccggtgggtg gcgaggtaga aggcccgggt gatcttgaga
     9841 tccttcacgc ggagacacca gaggctgccc gcccgacact cctcctcgac ggcccgccgc
     9901 gacacgagcg acacgccgac attcgccttc accgcctgct tgatcgcctg ggtcgagccc
     9961 atctccccga ccacgcggag ggccgcgacg tcgctgcccg cctgggtgag ggcggcctcg
    10021 aacgccgcgc gcgtgcccga gccgcgctcg cggagcagga gcggctcggc gcgcagctcc
    10081 tccagcgtga cctgcttgcg cccttgccag ggatgcgccg cgctcacgat gaggacgatg
    10141 tcgtcgggca gcagctcgcg gaagtcgatg ccccgatggc ccgatcgcgc gccgaccacg
    10201 cccacctcgg cccggccctc ggccacccac tccgtcacct cttggctgcc gccgatgagc
    10261 aaggtgatcg cgatgtcggg gaacttctcc ttgaagcggc cgatcagggc gggaaggatg
    10321 tactctccgg gaatcgtgct ggcgcccacg agcagctcgc cactcatccg cccctggaag
    10381 ctctcgatcg cctgccgcgc ttcgcgggac agggcgagga ggcgctgcgc gtagccctgg
    10441 agcagggcgc cgcccttggt gacggcggcc ccccggccga gccggtcgag cagacgcacg
    10501 cccagctcgt cctcgaggct tcggatgtgc tccgagacgg tgggctgcgt caggaacaat
    10561 gcgtcggcgg ccttggaaaa cgagccgagc tccgcgacct tgacgaagat ctcgagctgg
    10621 cgtagatcca cgggccgagt atagaagggc cccggggccg tgtaaagccg aggcccgtcg
    10681 ggggcgccgg tgttcagagt gtccagagac acgaaagggg cccgaaggcc cctcgtcggt
    10741 cgtggcggct cggtcgccgg ctcagcggca gccgcaccca cccccgcagc agccgccacc
    10801 acctccgccc atgggcgcgg gtgacgcgtc gctcatgcgc ccgccggtct tcacgccgac
    10861 gatggagagc cgccggctca cgcgctcgct ctcgcacgac gcgcatacca ccgggcccgg
    10921 gcgctgtacg agcttctcga actcggcccc gcaggccaag cacgcgtatt catagatggg
    10981 catgacgccg ctccgatctt tctcctgccc agtccgcggc tactgtccgc cgcgctggac
    11041 gtagctcttc acgaactcct cggcattctc ctcgaggata tcgtcgatct cgtccaggag
    11101 gttgtccagc tcctccttga tcttcttgcc cttcttggac agctcggcgc tacccgcgcc
    11161 gccgtcgtca ccaccctcgg cgggcttggt cggcctcgtt tccttcttct tctgctcagc
    11221 catgggacgt cacctcctgg ccccggtccg cggagccggc gtttcagggc ctagaccgac
    11281 ggcagcacga tcccctgctg gccctgatac ttcccctgcc tgtcagcata tgacgtctcg
    11341 caggcctcgt ccgattcaaa gaatagaacc tgcgcgatac cctcgttggc gtagattctc
    11401 gcggggagcg gcgtggtgtt gctgatctcg agcgtcacgt acccctccca ctccggctcg
    11461 aacggcgtca cgttcgtgat gatcccgcac cgcgcgtagg tcgacttccc cacgcagatc
    11521 gtcagcacgt tgcgggggat cttgaagtac tcgaccgacc gccccagcgc gaacgagttg
    11581 ggcggaatca ccgcgacctc gccctggtaa tcaacgaagg aacgcgggtc gaagctcttg
    11641 ggatccacga tcgtggtgtt gatgttggtg aagatcttga actcatccgc gatgcggatg
    11701 tcgtagccgt aggaggagag cccgtaggag atcccgccct tccgcacctg gcgatcctcg
    11761 aagggcttga tcatgccctt ctccacggcc atgcggcgga tccagcggtc gctcttgatc
    11821 acgagtacac cttccgtcga tcgtactgcc tgctccgagt gggagtcaag tctcgggcag
    11881 tctctcccac gtcgcgccct ccgtgtccac cggcagactg agcgcccggc cctcgatccc
    11941 ggcctcccgg agcgcgcgct ccatcgcgcg cgccacctcg tcgcctccgc cgcgggccgc
    12001 cgcgagcatg gacgggcccg cgccgctcag gacgcagccg agcgcgcccg cccgccgccc
    12061 cgcggcggcc accgcctcca gccacggaaa gagccgctgg cggtagggct ggtgcagccg
    12121 gtcctgcatg gcgaggtcga gcaggtccgg gcggccggcc ccgagcgcgg ccagcaacag
    12181 gctcacccgc tgcacgttga agaccgcgtc ggcccgcggc accgagtccg ggagcaccgc
    12241 ccgggcctcc cgcgtggagc tctccgtccc gggcagcagc accacccaga cgatctcgcg
    12301 cggcaccggc aggctcaccg cggcgacgcg cgcgccgtcg gcgcaggaca cggtgagccc
    12361 gcccagcacc gcggcggcca cgttgtccgg atggccctcg gcccgggcgg ccgcggtcac
    12421 gagcgcgtcg cgatcgaggg gctcgcccag cagcgcattg gccgcgacca ggccgccgac
    12481 ccatgcggcc gcactcgagc cgaggccccg gctcagcggg atgcggttga cgcagcgaag
    12541 gcgcccgccg cggaagggcc ggcccgccac ctcgaagccc agggcaacgc cgcgggcgac
    12601 cacgttcttc ccgtcgtcgt cgaggcgccc ggcaccctcg ccctccacgc tgacggagat
    12661 gccgtccgct tcctcgagga ccacctcgtt gtggagcccc agggccaggc ccagggtgtc
    12721 gaagcccggc ccgagattgg cggaggtggc gggcacacga acccggacgc gcatggcgca
    12781 gagaataccc cgggtccggg gtagaatgcg tgagaacggg ccggtcgcca atgcccaaga
    12841 ccatcatcga gccgcagttc acgatcgagc acctgtccat cctggacagt gacggcaacc
    12901 tggactccgc cctcgatcct gccatcgcgc ccgacgacct caagcgcctc taccgcgcca
    12961 tgctcctcgc ccggcgattc gacgagcgga tgctgcgcct gcagcgccag ggacgcatcg
    13021 gcaccttcgc gccgatcaag ggccaggaag cggcccagct cggcagcgtg gccgcgctgc
    13081 ggcggacgga ctggatggtg ccgtccttcc gcgagaccgc gtccatgctc tggcgcggct
    13141 ggcccatgga gaagatcctg ctcttcttct ccggctacct cgaaggcggg cgccccgagg
    13201 ccgatcagcg cgatctcccg atctgcatcc cggtcgccac ccagctcccg cacgcggtgg
    13261 gcctggccta cgccgcgcag taccgcggcg acgacgcggt ggtgatggtc tactgcggcg
    13321 atggggccac ctcggaaggt gacttccacg aggccttgaa cttcgcgggc gtctggcacg
    13381 tgccggtcgt cttcatcgtg cagaacaacc agtgggcgat ctccatcccg ctcaagaagc
    13441 agacccactc gcgcaccatc gcccagaagg cgctggccta cggccttccg ggcctgcagg
    13501 tggacggcaa cgacgtgctc gcagtgtacg cggccagcct cgaggcggtg gagcgggcgc
    13561 gccgcggcga cggcccgacc ctcatcgagt gcgtcaccta tcgcctcggc gtgcacacca
    13621 cctccgacga tcccaccaag taccgctcga ccgaggaggt ggaggcgtgg gagcgaaagg
    13681 agccgctcac ccgcttccgc ggttacctgc agcagcggag cctgctcgag cccggcctgg
    13741 aggaggcggt ggacgcggag atcgcgcgtg gcgtcgccgc cttcgaggcg gtcggtccgc
    13801 cggatccgct cggcatgttc gatcacgtct acggcaaccc gcccgccgcg ctcgccgccc
    13861 agcgggccga gatggcggcg cggctcggtg gcgcccccca gccggcggcg gagccgccgc
    13921 cgggcccggc gccgagcccg cccatgagag gacagaggcg ctcgtggcca agctgaacat
    13981 ggtgaaggcg ctgaacctgg c
//
LOCUS       DASUFA010000042         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1039900, whole genome shotgun sequence.
ACCESSION   DASUFA010000042 DASUFA010000000
VERSION     DASUFA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039900"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..924)
                     /gene="pdxS"
                     /locus_tag="VFX87_01560"
     CDS             complement(43..924)
                     /gene="pdxS"
                     /locus_tag="VFX87_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX4993618.1"
                     /translation="MESGTPRLKVGLAEMLKGGVIMDVTNAEQAKIAEDAGAVAVMAL
                     ERVPSDIRKEGGVARMSPVTKIKEIQKAVTIPVMAKCRIGHFVEAQILESLGVDFIDE
                     SEVLTPADEHFHVDKFAFKVPFVCGSRDLGECLRRIGEGAAMMRTKGEAGSGNIVEAV
                     RHMRAVTSGIRRLTMLGPEELMTEAKNLGAPYDLVRWVAKSGHLPVPNFSAGGIATPA
                     DAALMMQLGAEAVFVGSGIFKSSDPAVRAKAIVQATTHFKDPDVLAKVSEGLGDAMPG
                     IETSKLKDSELLQTRGW"
     gene            complement(957..1613)
                     /locus_tag="VFX87_01565"
     CDS             complement(957..1613)
                     /locus_tag="VFX87_01565"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX4993619.1"
                     /translation="MSAVGTERAPSVVTPVTLEGRWVRLEPLSRSHAAGLLAAASGSR
                     DTYGFTLVPGTAAESAAYIDAALAEQDAGRALPFATVDRARGVVVGCTRLFNIEFWPW
                     PPGNVNQRGADRPDVAEIGWTWLAAAAQRTPINTEAKLLMLAHAFDHWRVHRVSLMTD
                     ARNQRSRNAIMRLGARFDGVLRAARPASDGAIRDTAAFSILEAEWPAVRANLEARLKQ
                     "
     gene            complement(1610..>2213)
                     /locus_tag="VFX87_01570"
     CDS             complement(1610..>2213)
                     /locus_tag="VFX87_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX4993620.1"
                     /translation="FRPLEHGATMVMHSLTKFISGHGDVTGGLVAGRAEPMARVRNAM
                     IRAGTNLGPFDAWLATRGARTLAVRMERQCATALTLARGLERLPAVSRVYYPGLASHP
                     QHALAGRLMPKLSGAMVSFDLRGGAPAVERFMARARLLEFAPSFGDVATTWTYPARTS
                     HRRVSDEEKAAMGIGAGLVRVSVGLEDAAELMADLEGAVA"
BASE COUNT          302 a          806 c          774 g          331 t
ORIGIN      
        1 gattcggcgg gagctggtgg aggttggcgg aggtctcggc cactaccagc ctctggtctg
       61 gagcagctcg gagtccttca gctttgaagt ctcgatgccg ggcatggcgt cgccgagtcc
      121 ttcggagacc ttggccagca cgtcggggtc cttgaagtgc gtggtggcct gcacgatggc
      181 cttggcccgc accgccgggt ccgaggactt gaagatgccg gagcccacga agacggcctc
      241 ggcgccgagc tgcatcatga gcgccgcgtc ggccggagtg gcgatgccgc ccgccgagaa
      301 gttcggcacc ggcaggtgcc cgctcttggc cacccagcgc accaggtcgt agggggcgcc
      361 caggttcttg gcctcggtca tcagctcctc ggggcccagc atggtgagcc gacggatgcc
      421 cgacgtcacc gcgcgcatgt ggcgcaccgc ctcgacgatg ttgccggagc ccgcctcgcc
      481 cttggtccgc atcatggccg cgccctcgcc gatgcggcgc aggcactcgc ccagatcgcg
      541 cgatccgcag acgaagggca ccttgaaggc gaacttgtcc acgtggaagt gctcgtcggc
      601 gggggtgagc acctcggact cgtcgatgaa gtccaccccg agcgactcga ggatctgggc
      661 ctccacgaag tgcccgatcc ggcacttggc catcaccggg atcgtgaccg ccttctggat
      721 ctccttgatc ttggtgaccg gcgacatgcg ggccaccccg ccttccttcc ggatgtccga
      781 cggcacgcgc tccagcgcca tcaccgcgac ggctcccgcg tcctcggcga tcttggcctg
      841 ctccgcgttg gtgacgtcca tgatgacgcc gcccttcagc atctcggcca gccccacctt
      901 cagtctcggc gttccgctct ccatgtcctc gtctcctttg atgtactcgg ctcgggtcac
      961 tgcttgagtc gcgcttcgag attggctctg accgccggcc actccgcctc caggatggag
     1021 aaggccgcgg tgtcgcgaat ggcgccgtcg ctcgcgggcc gggccgcccg cagaacgccg
     1081 tcgaaccggg cgcccaggcg catgatcgcg ttccgcgagc gctggttgcg cgcgtcggtc
     1141 atgaggctca cgcgatggac ccgccagtgg tcgaacgcgt gggccagcat caacagcttc
     1201 gcttcggtgt tgatcggggt ccgctgcgcg gcggcggcca gccaggtcca gccgatctcg
     1261 gccacgtcgg gccggtccgc cccgcgctgg ttgacgttgc cgggcggcca cggccagaac
     1321 tcgatgttga agagccgcgt gcagccgacg accacgcccc gcgcccgatc caccgtggcg
     1381 aacgggagcg cgcggcccgc gtcctgctcg gcgagcgcgg cgtcgatgta ggcggcgctc
     1441 tcggccgcgg tcccgggcac cagcgtgaag ccgtaggtgt cccgcgagcc cgaggcggca
     1501 gcgagcaggc ccgccgcgtg cgaccggctg agcggctcga gccggaccca gcgcccctcc
     1561 agggtgaccg gcgtcaccac cgacggcgcg cgctccgtcc cgaccgcgct catgcgacgg
     1621 ctccctcgag atcggccatc agctcggccg catcctcgag cccgaccgag acacggacga
     1681 ggccggcgcc gatccccatc gcggccttct cttcgtcgga gacgcgccgg tgtgacgtgc
     1741 gggccggata cgtccacgtg gtggccacgt cgccgaagct cggcgcgaac tcgagcagcc
     1801 gcgcccgcgc catgaagcgc tccaccgccg gcgcgccgcc ccgcaggtcg aaggaaacca
     1861 tcgcgccgga gagcttcggc atcaggcgcc ccgcgagcgc gtgctgggga tgcgaagcca
     1921 ggcccggata gtagaccctc gataccgcgg gcaggcgctc gagcccccgg gccaaggtca
     1981 gcgcggtggc gcactgccgc tccatgcgca ccgcgagggt gcgggcgccg cgggtcgcca
     2041 gccacgcgtc gaacgggccc aggttggtgc ccgcgcggat catcgcgttg cggacgcgcg
     2101 ccatcggctc ggcgcggccg gccacgagcc cgccggtgac gtcgccgtgg ccggagatga
     2161 acttggtgag gctgtgcatc accatggtcg ccccgtgctc gagcgggcgg aag
//
LOCUS       DASUFA010000043        14921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_696696, whole genome shotgun sequence.
ACCESSION   DASUFA010000043 DASUFA010000000
VERSION     DASUFA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696696"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFX87_01575"
     CDS             complement(<1..258)
                     /locus_tag="VFX87_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993621.1"
                     /translation="MALLSELTALRARVAASPGRLEKRRLVADYLRGLTPEDLRYAVT
                     YLSGRAFPASDPRVLNVRGLPAPGPGLSPSGLSPSGLSPSGL"
     gene            complement(312..1550)
                     /locus_tag="VFX87_01580"
     CDS             complement(312..1550)
                     /locus_tag="VFX87_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEX4993622.1"
                     /translation="MAELYLERLIALVAFVGTYLGLALGRLPFFRVDRTGVAIIGGAL
                     VVVTGVLPWEKAVAAVDAHTLVLLFGMMIVAAYLRLSGFFRLVTYAAVRRAHTPVGLL
                     ALIIVAAGVLSALFVNDVVCLVMAPIVLDLTRQVRLPPVPYLIALATAANVGSVATLT
                     GNPQNMLVGSFSGISYRAFLVREAPVAIIGLALVLVVIWLVYRRQLPDALPAANLEDR
                     FAVHYPLMIKTIAAVSVMLVAFLAGVPIALVAIGGAAYTLLTRRVNPEKVYREIDWGL
                     LVLFTGLFVVIGAVEQTGLAAEVLGWASAAGLHRPLVLTVVTAALSNVVSNVPAVLLF
                     KSVIPTFGEPGRAWLLLAMASTLAGNLTILGSVANLIVVEQARSAGIPVGFLEYSKVG
                     VPVTVLTLLVGWLLLVLLPV"
     gene            complement(1572..3146)
                     /gene="ggt"
                     /locus_tag="VFX87_01585"
     CDS             complement(1572..3146)
                     /gene="ggt"
                     /locus_tag="VFX87_01585"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4993623.1"
                     /translation="MVTSPHALASQAGVAILQAGGSAVDAAIAAAAVLSVVYPHMTSL
                     GGDAFWLIHDASTGAVRSLAAAGRAAAAVSMDWFAARGLREIPVRGIVPATLTVPGAV
                     DGWCQAHAAHGRLPLATDLAAAVGYARDGFPITQRLAQWITRSADVLAADPAAARIFL
                     AGGPSPATGRRLTNPDLARTLERVVEGRGGFYEGETAREMARHAREHGGFFTERDFAA
                     QRATWGASIHAAYRGVTIHETPPPSQGVSVLQALRLLEPWEIGALPFLGPDHVHLLVQ
                     AKQIAFHDRDRLVADPDFSPVPVERLLSRAYADERRVLMDRERALPWDRVPSSGSLAG
                     DTVYVAAVDAEGNAVSLIQSLYFGFGSGVVAGNTGVVLQNRGAYFSLDPAHPNRLEPG
                     KRPLHTLIASLAFKDGRLWQVLGCMGADGQPQIHLQVYTAMIDFGLDIQQAIEAPRWL
                     AGRFALGDAREVLNVEGRFAPATIAELARRGHEINRWEAWNELAGHAHGITIDPESGT
                     RAGGADPRSDGAAVGY"
     gene            complement(3236..3739)
                     /locus_tag="VFX87_01590"
     CDS             complement(3236..3739)
                     /locus_tag="VFX87_01590"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4993624.1"
                     /translation="MPGSGSPAAPAGDYQVRRAGPADHDGVVRELAAYFAFLGEDVDA
                     DGLDHDVADWQREYDGAAGVMLVVVDPAGDVVGTAAVRRLAPGVAELKRMWLRPDCRG
                     RGLAPRLMDRCLEEARALGGRVLRLDSERHRLAAAVKLYRRYGFEEIPDYNRNPRADV
                     WMELRLT"
     gene            complement(3723..4331)
                     /locus_tag="VFX87_01595"
     CDS             complement(3723..4331)
                     /locus_tag="VFX87_01595"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX4993625.1"
                     /translation="MPVRINRVYTRAGDAGETALVGGRRVPKDSPRIEAYGTIDELNS
                     IVGLARAFNAPKLKRGKNYRRLDEILRRVQNELFDLGSELATPREAFYEGMFRVGDFE
                     VTALEKLMDECQKELTPLKSFVLPGGGPVGGFLHQARTVCRRAERGVLALSRVEDIGE
                     WPLKYVNRLSDLFFVLSRWVGKHLGETEYLWERGLASHARKR"
     gene            4379..4819
                     /locus_tag="VFX87_01600"
     CDS             4379..4819
                     /locus_tag="VFX87_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX4993626.1"
                     /translation="MSDWRDNLIATPEDIARVVRESKRIAVLGIKTEAQRGQPAFYVP
                     EYMARAGYDIVPVPVYYPEVTEILGRPVYRTVSAVPGHVDMVNVFRRPRDIPPHVQDI
                     LAARPRVVWLQLGIRHDEAAETFAKAGIQVVQDRCLLVEHRRLQ"
     gene            complement(4843..6903)
                     /locus_tag="VFX87_01605"
     CDS             complement(4843..6903)
                     /locus_tag="VFX87_01605"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX4993627.1"
                     /translation="MPALLALRLVTYLMVCTGVAALYLANLVGAPGAALVVLAILVSW
                     GHEQARERGAVRPALGWSLVGAAAVAIAVDLFYLAYSLLDGMVHLLLFLILLRLFARR
                     GLRDLRDAGLLSFFTLVAAASISFNLSFLFVFIAFFLLGTWMLILNHVVTELELAGRP
                     GDVATTARRVFFRGPLTQVSLVAAAITFSLAAGLFFVIPRVGQAALPLRASIGRMVTG
                     FSDRVDLGSFGDIESDRSVVMRVYLADEWPDPTTLPGLRWRGVAFDQFDGQTWSSTAA
                     PRRILHRSVTGDFGLGLPRGTGPVLRQEIYLEPIGSDAVFAAPRAVRIEMRSPNVVVD
                     DMDGLAVSSPGARLHYVVESEIESPGRPPGPGRRASATLPAADRERFLQLPPLPPEIA
                     RLAREVTAGSVDAGAAADRISAHLSTNYRYTLALQRQTALAPLEEFLFVRRSGNCEYF
                     AAAMVVMLRSIGVPARVVAGFQQGEWNPYGRYFMVRLSDAHAWVEVHLDGRGWVAFDP
                     SPRADAAAEEIPGRMSLYLDAARMRWYRHVVSWSLRDQVQLAASVHRQAADARLALAW
                     PRDWRVSPAVGTGAGLAVVGALLWWLQRHGLVLPRSGPAARMPAFYERALRLLARRGL
                     APGAAETARQFAARVEERAPDRAAAFGRLTSYYERLRFGDTPLSEAERQDVLRSLAAL
                     SARS"
     gene            complement(6905..7945)
                     /locus_tag="VFX87_01610"
     CDS             complement(6905..7945)
                     /locus_tag="VFX87_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4993628.1"
                     /translation="MTLVRAALWRLFHPRRTIKPTRDGWWCLLVAVGLGVAAINTGNN
                     LLYLLVSALLALIVVSGILSEQSMRGLDLTGIAPDEIYAGQPAVLGATIANRKRWFTS
                     YSVTVELLAPEARSLESASRFIYLRRIEAGLERLATWEETLPHRGRHRLTGVRMTTRF
                     PFGLFVKAGRPVLTDDVVVFPAVRPVSAESLRRLGESGDASARRRGRGNDLSNLRGYR
                     SGDDPRFIHWRSSAKAEGLMVRELEADTSQNTRLVLVGRGRRGAEALERGLSEAASLA
                     VALIRAGAGVELAGPDCAVPLGHGRAHLRRMLTALALYDAEAPRAEPAAPTGAEALRG
                     HRPLREIRIALA"
     gene            complement(7950..8885)
                     /locus_tag="VFX87_01615"
     CDS             complement(7950..8885)
                     /locus_tag="VFX87_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4993629.1"
                     /translation="MGDSTETIRRLEENVGRALFGKPEVVRLAIIGLVARGHLLIEDV
                     PGVGKTTLASGLARSIGASFQRIQFTSDMLPSDVLGVSVWEPKSGEFVFRPGPLFTNI
                     VLADEINRTTPKTQSALLEAMNEAQVSLDHSTYPLPRPFMVLATQNPREYEGTFPLPE
                     SQLDRFLLRIRIGYPGAADEKAVLRGDTAPADLMAVVEVDEVLALQEAAEKVRTEESV
                     LDYLVALVTATRRSPLLALGVSPRGSLALLRAARARALADGRDYLLPDDIKHLAVPAL
                     AHRVIVKGSLAGSAAGGMDGEAAVTAILQDVPVPR"
     gene            8973..9800
                     /locus_tag="VFX87_01620"
     CDS             8973..9800
                     /locus_tag="VFX87_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX4993630.1"
                     /translation="MALDPPPAPHWPRNARAACAFTFDLDAETLWMARGVHEPVTLSQ
                     GRFGPVEALPRILALLRTHDIRASFFIPAWVAEHHQDAVRAIVDGGHEIGCHGDEHER
                     VSELPPDREEAILARSVEVLTRLNGTRPIGYRAPAWQLSAATIPLLVQYGFAYSSNMM
                     DRLSPYLHPAVGGRAIVEIPVSWILDDAPYFMFTGQRSIQPPGPVLQGWLTEFEGITE
                     ARGVTNFTFHPQIIGRPSRLACLRELIAHVRHAPRVWIASLGEISAHYRTSVESGAG"
     gene            complement(9797..10732)
                     /locus_tag="VFX87_01625"
     CDS             complement(9797..10732)
                     /locus_tag="VFX87_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4993631.1"
                     /translation="MKFALFTGMTGTTWDEVLALWRHGDQTGWDAACVTDHFMPNTAD
                     RVGDTLECWTTLAALAGLTTRLRVGTIVSGNTYRHPALLAKMATNVDIISRGRLICGV
                     GAGWQENEHRAYGMDFYTTRERLARLDEACHVMKALWTGTKADYKGKYYQLEAAPLMP
                     KPVQKPYPELMIGGGGEKVTLRIAARHADHWNVWGGPATLTHKGGILDQHCATEKRDP
                     KSILRSAVMVLGFADDRAGVEKIEQAYMKRMGADAEKARDTVLAGSVGQIKDKLARLR
                     EAGVGMLFIPTFLLPADRLSTLDRFMAEVAPAFRA"
     gene            10771..11676
                     /locus_tag="VFX87_01630"
     CDS             10771..11676
                     /locus_tag="VFX87_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX4993632.1"
                     /translation="METGFLVIHPGALGDVLQAVPALRGLRAAAPAAPITFVGQPRLA
                     RLLVDLGAAQHARTFDGFGLEALFVDAPTPPALAEAVSRVKNVVSWFGSRDETYRRRL
                     RALAPRALVAPPVPDDDTPVWRHLLGTLAGWDLAIPERVEPLRAPPLPASDAAADAGR
                     PRLVVHPGSGGDWKRWPAERFAEVIRALRRRRAFEVLVHQGPADAEPAQRLLTLLEGD
                     AAVLLQPELTRLAAVLGAARAYLGGDSGVSHLAAAVGTPSVVLFPSATYRRWTPWSPT
                     AIPVEIQGMDGDVARALEALALVLA"
     gene            complement(11698..12018)
                     /locus_tag="VFX87_01635"
     CDS             complement(11698..12018)
                     /locus_tag="VFX87_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX4993633.1"
                     /translation="MKRSIALLVVALFSAGVLSAPAAWAQAPKTAPPPAKSEMKATGH
                     DPLDLNTASEADLKTIPGIGDAYAKKIVQNRPYKRKDELVQKKLVPQATYDKIKDHVV
                     AKQK"
     gene            complement(12056..12403)
                     /locus_tag="VFX87_01640"
                     /pseudo
     CDS             complement(12056..12403)
                     /locus_tag="VFX87_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(12440..13114)
                     /locus_tag="VFX87_01645"
     CDS             complement(12440..13114)
                     /locus_tag="VFX87_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014291852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX4993634.1"
                     /translation="MRLAIVIPALNEAAHLARLLPDLARDCPGAEIVVVDGGSRDGTA
                     AVVTREPGPRLLASARGRALQMNHGARAAAGDTLLFLHADTRLPDGAARAIEQALAAP
                     GVVGGRFDVRFDNEGALFRTIAWCMNTRSRASGICTGDQAIFVRRADFEAIGGYPEIP
                     LMEDIELSRRLKRRGRMRALRLRVTTSARKWEREGPLRTIGLMWALRFLHFCGVAPAR
                     LHRWYY"
     gene            complement(13111..13860)
                     /locus_tag="VFX87_01650"
     CDS             complement(13111..13860)
                     /locus_tag="VFX87_01650"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX4993635.1"
                     /translation="MRTPRAGLGRVGAALLGLLLAAAAGRAAEPLRLGDFAPPTDATA
                     PPPGWEPLTFPRVSRHTRYTVVLEGERWVLRAESDAAAAGLFHPLDLDPKVYRVLSWR
                     WKVEGVLAKSDPRRKQGDDYVARVYVAYRYDPAAASRWERARFGAYRVLYGRYPPGLA
                     LNYVWESRLPVGTVLDNAYTARARILVARSGAAEVGRWVTETRDIYEDFRRIVGGEPP
                     RIVGIALMTDTDDTGERAVAYYDALTIGPGR"
     gene            complement(13857..14600)
                     /locus_tag="VFX87_01655"
     CDS             complement(13857..14600)
                     /locus_tag="VFX87_01655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX4993636.1"
                     /translation="MDHLPREPPRPRARPGQVLSRRLLVVTLLALALAVVLVRVLGLA
                     DLVRLENLARLRERIDGLGAWAPVVFILGYVVAVVAFVPALPLTILGGLAFGTAWGTV
                     YVAIAATTGACLAFLIARYAARAAVARWLAGYPALERMDRAVARHGFRIVMITRLVPI
                     FPFNLQNYAYGLTSIGFGAYALTSFLCMLPGTLAFTAAAGAVVAGAWDARRMLLLLAM
                     AGVLLVALSLLPRWLRGRSAALDDLLRTG"
     gene            complement(14543..>14921)
                     /locus_tag="VFX87_01660"
     CDS             complement(14543..>14921)
                     /locus_tag="VFX87_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4993637.1"
                     /translation="EFLWESATPNGLLRHLMERAEADVMLCTHTGIKWHRALPDARHA
                     VNVGVIGRPENDGRTNVWYTVLTAGPDLQVEFVPLAYDHETLAREMDREGLPPEFAET
                     IRTGWWTTCLENLPARERARGKY"
BASE COUNT         2160 a         5450 c         5405 g         1906 t
ORIGIN      
        1 gaggcccgaa ggtgagaggc ccgaaggtga gaggcccgaa ggtgagaggc ccggccccgg
       61 cgcgggcagg ccacgcacgt tcagcacccg cgggtccgaa gcggggaacg cgcggccgct
      121 caggtaggtg acggcgtacc gcaggtcctc gggcgtaagg ccgcgcaggt agtcggcgac
      181 caggcgccgc ttctcgagac ggcccgggct cgcggcgacg cgggcgcgca gagccgtgag
      241 ttccgagagc agggccatgc gcgcattatc cctcgcggcc cctcaccctg ccctctcccc
      301 agaggggaga cctacaccgg gagcaggacg aggaggagcc agcccaccag gagggtcagc
      361 acggtgaccg gcacgcccac cttgctgtac tcgaggaagc ccaccgggat gcccgcgctg
      421 cgagcctgct ccaccacgat caggttggcc accgagccca ggatcgtgag gttgcccgcg
      481 agcgtcgagg ccatggcgag cagcagccag gcgcgccccg gctcgccgaa ggtcgggatc
      541 acgctcttga agaggagcac cgccgggacg ttggagacca cgttcgagag cgctgcggtg
      601 accacggtga gcacgagcgg ccggtggagg cccgccgcgg aggcccagcc gaggacctcg
      661 gcggcgaggc ccgtctgctc cacggcgccg atgaccacga agaggccggt gaagagcacg
      721 agcagccccc agtcgatctc gcggtacacc ttctccggat tcacccgccg cgtgagcagg
      781 gtgtaggcgg cgcccccgat ggccaccagc gcgatcggca cgccggccag gaaggccacc
      841 agcatgactg acaccgccgc gatggtcttg atcatcaggg ggtagtgcac cgcgaagcgg
      901 tcctccaggt tcgcggcggg cagcgcgtcg gggagctgcc ggcgatacac gagccagatc
      961 acgacgagca ccagcgcgag gccgatgatc gcgaccggcg cctcgcgcac caggaaggcg
     1021 cggtagctga tgccggagaa gctgcccacc agcatgttct gcgggttgcc ggtcagcgtc
     1081 gccacgctac ccacgttggc cgcagtggcg agcgcgatca ggtacggcac cggcggcagc
     1141 cggacctggc gcgtgaggtc cagcacgatg ggcgccatca cgagacacac cacgtcgttg
     1201 acgaagagcg cggagagcac acccgccgcg acgatgatca gggccagcag cccgaccggg
     1261 gtgtgggcgc ggcgcaccgc cgcgtaggtc acgaggcgga agaagccgga gaggcgcagg
     1321 taggccgcca cgatcatcat gccgaagagg agcacgaggg tgtgggcgtc caccgcggcc
     1381 acggccttct cccacggcag gacgccggtc accacgacga gcgcgccgcc gatgatggcg
     1441 acgccggtgc gatccacgcg gaagaacggc aggcggccga gcgccaggcc caggtaggtt
     1501 ccgacgaagg ccaccagggc gatcagccgc tcgaggtaca gctcagccat cccccctccg
     1561 accgcgcccg gtcagtaacc gacggcggcg ccgtcgctcc gcggatcggc tccgcccgcc
     1621 cgcgtcccgc tctcgggatc gatcgtgatg ccgtgggcgt gcccggcgag ctcgttccac
     1681 gcttcccagc gattgatctc atggccccgc cgcgccagct cggcgatggt ggccggcgcg
     1741 aaccggccct ccacgttcag cacttcgcgc gcgtcgccca gggcgaagcg cccggcgagc
     1801 cagcggggcg cctcgatggc ctgctggatg tcgagcccga agtcgatcat cgcggtgtag
     1861 acctgcaggt ggatctgggg ctggccgtcg gcgcccatgc agccgagcac ctgccagagc
     1921 cgcccatcct tgaaggccag cgacgcgatc agcgtgtgca gcggccgctt gccgggctcg
     1981 agccggttgg gatgggcggg atcgagcgag aagtaggcgc cgcggttctg gagcacgacg
     2041 ccggtgttgc ccgccaccac gcccgacccg aagccgaagt agaggctctg gatcagcgag
     2101 accgcgttcc cctcggcgtc caccgccgcg acgtagaccg tatcgccggc caggctgcca
     2161 ctggacggga cgcggtccca gggcaacgcc cgctcccggt ccatgaggac ccggcgctcg
     2221 tccgcgtacg cccgtgacag cagccgctcg acgggcaccg gggagaagtc gggatcggcg
     2281 acgagccggt cccggtcgtg gaaggcgatc tgcttggcct gcacgagcag gtgcacgtgg
     2341 tccggcccca ggaagggcag cgcgccgatc tcccagggct cgagcaggcg gagcgcctgc
     2401 agcaccgaca cgccctggct cggcggcggc gtctcgtgga tggtgacgcc gcgataggcg
     2461 gcgtggatgg aggcgcccca cgtcgcgcgc tgcgcggcga agtcgcgctc ggtgaagaag
     2521 ccgccgtgct cgcgcgcgtg ccgcgccatc tcgcgcgcgg tctccccctc gtagaagccg
     2581 ccccgcccct cgaccacgcg ttcgagcgtg cgcgcgaggt ccgggttggt cagccgccgt
     2641 cccgtcgccg ggctcggccc gccggccagg aagatgcgcg cagccgccgg atccgccgcg
     2701 agcacgtcgg ccgagcgtgt gatccactgc gcgagccgtt gcgtgatcgg gaagccgtcg
     2761 cgcgcgtagc cgacggcggc ggccaggtcg gtggcgagcg ggagccggcc gtgggccgcg
     2821 tgcgcctggc accagccgtc caccgcgccg ggcacggtga gcgtggcggg cacgatcccg
     2881 cggaccggga tctcgcggag cccgcgggcc gcgaaccagt ccatgctcac cgcggccgcc
     2941 gcacggcccg ccgccgccag cgagcgcacg gcgccggtgc tcgcgtcgtg gatcagccag
     3001 aaggcgtcgc cgccgaggct cgtcatgtgg gggtagacga cggagaggac ggcggcggcc
     3061 gcgatcgccg catccaccgc ggagcctccg gcctgcagga tcgcgacgcc cgcctgggac
     3121 gccagcgcgt ggggcgacgt gaccatgccg cggtacgcga gtgtggccgg ccggccggtc
     3181 tccaggatgc gaatccttct ctctccctcc cagggagagg acggggtgag ggtggctacg
     3241 tgagccgcag ctccatccag acgtcggccc gcggattgcg gttgtagtcc gggatctcct
     3301 cgaacccgta acggcgatag agcttcaccg ccgcggccag gcgatgtcgc tcgctgtcca
     3361 gccgcagcac gcggcccccc agggcccgcg cctcctcgag gcagcggtcc atcaggcgcg
     3421 gcgccagccc gcgcccgcgg cagtccgggc gcagccacat gcgcttgagc tcggccacac
     3481 ccggggccag gcggcgcacc gccgcggtgc ccaccacgtc acccgcggga tccaccacga
     3541 ccagcatcac gcccgcggcc ccgtcatact cgcgctgcca gtccgccacg tcgtggtcga
     3601 gcccgtcggc gtccacgtcc tcgcccagga aggcgaaata cgcggcgagc tcgcgcacga
     3661 cgccgtcgtg atcggccggg cccgcgcgac gcacctggta gtcgccggcc ggcgcggccg
     3721 ggctaccgct tccgggcatg cgatgcgaga ccgcgctccc agaggtactc cgtctcgccc
     3781 agatgcttgc cgacccagcg cgagagcacg aagaacaggt cgctgagccg gttcacgtac
     3841 ttcagcggcc actcgccgat atcctccacc cgcgagaggg cgagcacgcc gcgctcggcg
     3901 cgccggcaca ccgtgcgggc ctggtggagg aacccgccca ccggcccgcc gcccggcagc
     3961 acgaaggact tgaggggggt gagctccttc tggcactcgt ccatcagctt ctcgagcgcg
     4021 gtgacctcga agtcacccac gcggaacatg ccctcgtaga acgcctcgcg cggggtggcc
     4081 agctcgctgc ccagatcgaa gagctcattc tgcacccggc gcaggatctc gtcgagccgg
     4141 cggtagttct tgccgcgctt gagcttcggg gcgttgaagg cccgcgccag gccgacgatc
     4201 gagttcagct cgtcgatggt gccgtacgct tcgatgcgcg gcgagtcctt gggcacgcgg
     4261 cgcccgccca cgagggcggt ctcgccggcg tcaccggcgc gcgtgtagac gcgattgatg
     4321 cggacgggca tgacggctcc tcccgcgccg aggctagcac aggtgctaga ctctgaccat
     4381 gagcgactgg cgcgacaacc tcatcgcgac ccccgaagac atcgcgcgcg tggtccgcga
     4441 gagcaagcgc atcgcggtgc tggggatcaa gaccgaggcg cagcgtggcc agcccgcgtt
     4501 ctacgtgccc gagtacatgg cgcgggccgg ctacgacatc gtgcccgtgc cggtctacta
     4561 cccggaggtg acggagatcc tcggccgccc cgtctaccgc acggtgagcg cggtgcccgg
     4621 gcatgtcgac atggtcaacg tgttccggcg cccgcgcgac atcccgccgc acgtgcagga
     4681 catcctggcc gcgcggcccc gggtggtctg gctgcagctc ggcatccgcc acgacgaagc
     4741 cgccgagacc ttcgcgaagg cggggatcca ggtcgtccag gaccgctgtc tgctggtgga
     4801 gcaccgccgg ctgcagtagt cggcggggag gcggcagatg acctacgatc gcgcgctgag
     4861 ggcggcgagc gagcgcaaca cgtcctgccg ctccgcctca ctgagcggcg tgtcgccgaa
     4921 gcgcaaacgc tcgtagtagc tcgtgagccg gccgaacgcg gccgcgcggt ccggcgcgcg
     4981 ctcctccacc cgcgcggcga actgccgcgc ggtttccgcg gcgccgggcg ccagcccgcg
     5041 gcgggccagg agccggaggg cgcgctcgta gaacgcgggc atccgcgccg ccgggccgct
     5101 ccgcggcagg acgagcccgt ggcgctggag ccaccacagc agcgccccca ccaccgcgag
     5161 gcccgcgccg gtcccgaccg ccgggctcac gcgccagtcg cggggccacg cgagcgccag
     5221 gcgcgcgtcg gccgcctgac ggtggacgga ggcggcgagc tgcacctggt cgcgcaggct
     5281 ccagctgacc acgtggcgat accagcgcat gcgcgcggcg tcgagataga gcgacatccg
     5341 gccggggatc tcctccgccg ccgcgtccgc gcgcggcgaa ggatcgaagg ccacccagcc
     5401 ccgcccgtcg aggtggacct cgacccaggc gtgggcgtcg ctcaggcgca ccatgaagta
     5461 acggccgtac gggttccact cgccctgctg gaagcccgcc accacccggg cgggcacgcc
     5521 gatgctccgc agcatgacca ccatcgcggc cgcgaagtac tcacagtttc ccgagcggcg
     5581 cacgaacagg aactcctcga gcggcgccag tgcggtctgc cgctggagcg ccagcgtgta
     5641 acgatagttg gtgctgagat gcgcgctgat ccggtcggcc gccgccccgg cgtcgacgct
     5701 gcccgcggtc acctcacggg cgaggcgcgc gatctcgggc ggcaggggcg ggagctgcag
     5761 gaaccgctcg cgatcggcgg cggggagcgt ggccgacgcg cgacggcccg ggcccggcgg
     5821 ccggccgggg ctctcgatct cggactcgac gacgtagtgc aggcgggcgc cgggactcga
     5881 cacggcgagg ccgtccatgt cgtccaccac cacgttgggc gagcgcatct cgatccgcac
     5941 cgcgcgcggc gccgcgaaca ccgcgtcgct gccgatcggc tccagataga tctcctggcg
     6001 cagcaccggg cccgttccgc ggggcagccc gaggccgaaa tcgccggtga ccgagcggtg
     6061 caggattcgc cgcggcgccg ccgtgctcga ccacgtctgg ccgtcgaact ggtcgaacgc
     6121 cacgccgcgc cagcgcagcc cgggcagcgt ggtcggatcg ggccactcgt ccgccaggta
     6181 gacgcgcatg accacgctgc ggtcgctctc gatgtcgccg aagctgccca ggtccacgcg
     6241 gtccgagaat ccggtcacca tgcgcccgat ggaggcccgc agcggcagcg cggcctgccc
     6301 cacccgcggg atcacgaaga agaggccggc cgcgagcgag aaggtgatgg cggcggccac
     6361 caggctcacc tgggtgagcg ggccgcggaa gaagacccgg cgggcggtgg tggcgacgtc
     6421 gccggggcgg cccgccagct cgagctcggt caccacgtgg ttgaggatca gcatccaggt
     6481 gccgagcagg aagaacgcga tgaacacgaa cagaaagctc aggttgaagc tgatcgaggc
     6541 ggcggccacc agcgtgaaga aggacagcag cccggcatcg cgcaggtccc gcaggccgcg
     6601 gcgcgcgaag agccgcagca ggatgaggaa cagcagcagg tggaccatgc cgtccagcag
     6661 cgagtacgcc aggtagaaca ggtcgaccgc gatggccacc gccgcggcgc caaccaggct
     6721 ccagccgagc gccggccgca ccgcgccgcg ctcgcgcgcc tgctcgtggc cccagctcac
     6781 caggatcgcc agcaccacca gcgcggcgcc cggcgccccg accaggttcg cgaggtagag
     6841 cgcggccacg ccggtgcaga ccatcaggta cgtgacgagg cggagggcga ggagagccgg
     6901 catgtcaggc cagcgcgatc cggatctcgc ggagcggacg gtggccgcga agcgcttccg
     6961 cgcccgtggg cgccgccggc tcggcgcgcg gggcctccgc gtcgtagagc gcgagcgcgg
     7021 tgagcatccg ccgcaggtgc gcgcggccgt ggccgagcgg caccgcgcag tccggccccg
     7081 ccagctccac ccccgcgccc gcccggatga gcgcgaccgc cagggaggcc gcctccgaga
     7141 gcccgcgctc gagcgcctcc gcgccgcgcc ggccccgccc gaccagcacg aggcgcgtgt
     7201 tctggctcgt gtccgcctcg agctcgcgca ccatgagccc ttccgccttg gcgctgctgc
     7261 gccagtggat gaagcgcggg tcgtcgcccg accggtagcc gcggagattg gagagatcgt
     7321 tgccgcggcc ccggcgccgc gcggaggcgt cgccgctctc gccgagccgg cggagcgact
     7381 cggcggagac cgggcgcacc gcggggaaca cgaccacgtc gtcggtaagg acgggccgcc
     7441 ccgccttcac gaagaggccg aaggggaagc gcgtggtcat gcgcaccccg gtgaggcggt
     7501 ggcggccgcg gtgcggcagc gtctcctccc aggtggcgag gcgctcgagc cccgcctcga
     7561 tccggcgcag gtagatgaag cgtgaggcgg actcgagcga gcgcgcctcc ggggcgagca
     7621 gctccaccgt gaccgagtac gaggtgaacc agcgcttccg gttggcgatc gtggccccga
     7681 ggaccgcggg ctggcccgcg tagatctcgt ccggcgcgat gccggtcagg tcgaggcccc
     7741 gcatgctctg ctccgagagg atgcccgaga ccacgatgag ggccagcaat gctgagacca
     7801 gcaggtagag caggttgttg ccggtattga tggcggccac gcccagcccc accgccacga
     7861 gcaggcacca ccagccgtcg cgggtgggct tgatggtccg gcgcgggtgg aacagccgcc
     7921 agagcgcggc ccggaccagc gtcaccgccc tacctcggga cgggaacgtc ctgcaggatg
     7981 gcggtgacgg cggcttcgcc gtccatgccg cccgccgcgg agcccgcgag cgagcccttg
     8041 acgatcacgc ggtgggcgag ggccggcacc gccaggtgct tgatgtcgtc gggcagcagg
     8101 tagtcgcgcc cgtcggcgag cgcgcgggcg cgggcggccc gcagcagcgc cagcgagccg
     8161 cgcgggctca cgcccagcgc gagcagcggc gagcggcgcg tcgcggtgac cagcgccacc
     8221 aggtagtcga ggaccgattc ctccgtccgc accttctcgg ccgcctcctg cagcgccagt
     8281 acctcgtcca cctcgaccac cgccatgagg tcggcgggtg cggtgtcgcc gcggagcacc
     8341 gccttctcgt cggcggcgcc cggatagccg atacggatgc gcagcaggaa gcggtcgagc
     8401 tgcgattcgg gcagcgggaa cgtgccctcg tactcgcgcg ggttctgggt ggccagcacc
     8461 atgaagggcc ggggcaacgg atacgtcgag tgatcgagcg agacctgagc ctcgttcatc
     8521 gcctcgagca gcgcggactg cgtcttcggc gtggtgcggt tgatctcgtc ggcgagcacg
     8581 atgttggtga agagggggcc cggcctgaag acgaactccc cgctcttggg ctcccacacc
     8641 gagacgccca gcacgtcgga gggcagcatg tcggaagtga actggatgcg ctggaacgag
     8701 gcgccgatgg accgggccag gcccgaggcc agcgtggtct tgcccacgcc gggcacgtcc
     8761 tcgatcagca ggtgcccgcg ggcgaccagg ccgatgatgg cgagccgcac cacctccggc
     8821 ttgccgaaca gcgcgcggcc gacgttctcc tccagacggc ggatggtttc cgtcgagtcg
     8881 cccatgggat tccactatac cgtaggggca tttttcgacc cgcgcggggt cgctggccgg
     8941 ggcggccacc atcggctaca ctgggcgacg cgatggccct cgacccgccc ccggcacccc
     9001 actggccccg caacgcccgc gcggcctgcg ccttcacctt cgatctcgac gcggagacgc
     9061 tgtggatggc ccgcggcgtt cacgagccgg tcacgctctc ccaggggcgg ttcgggccgg
     9121 tggaagcgct gccgcgcatt ctcgcgctcc tgcggacgca cgacatccgc gcctcgttct
     9181 tcatcccggc ctgggtggcc gagcaccacc aggacgcggt gcgcgccatc gtggacggcg
     9241 gccacgagat cggctgccac ggcgacgagc acgagcgcgt gagcgaattg ccgccggacc
     9301 gcgaggaggc catcctggcc cgcagcgtcg aggtgctcac gcgcctgaac ggcacgcgcc
     9361 cgatcggcta ccgcgcgccc gcctggcagc tctccgccgc gacgatcccg ctgctcgtgc
     9421 aatacggctt cgcgtactcc tcgaacatga tggaccggct ctcgccgtac ctgcatcccg
     9481 cggtgggggg ccgtgcgatc gtggagatcc cggtctcgtg gatactcgac gacgccccct
     9541 acttcatgtt caccggccag cggtcgatcc agccgccggg cccggtgctg cagggctggc
     9601 tcaccgagtt cgagggcatc acggaggcgc ggggggtgac caacttcacc ttccacccgc
     9661 agatcatcgg ccggccctca cgcctggcct gcctccgcga gctgatcgcg cacgtgcgcc
     9721 acgctccgcg ggtgtggatc gcctccctcg gcgagatcag cgcgcactac cggacctcgg
     9781 tggagagcgg cgcgggctag gcgcggaagg cgggcgcgac ctcggccatg aagcgatcga
     9841 gagtggacag gcgatcggcg gggaggagga aggtcgggat gaagagcatg cccacgccgg
     9901 cctcccgcag ccgcgccagc ttgtccttga tctggcccac gctgcccgcc agcaccgtgt
     9961 cgcgcgcctt ctccgcgtcg gcgcccattc gcttcatgta ggcctgctcg atcttctcga
    10021 cacccgcgcg atcgtccgcg aagcccagca ccatcaccgc cgagcgcagg atgctcttcg
    10081 ggtcgcgctt ctccgtcgcg cagtgctgat cgaggatccc gcccttgtgg gtcaaggtgg
    10141 ccgggccgcc ccacacgttc cagtgatcgg cgtgccgggc cgcgatgcgc agcgtgacct
    10201 tctcgccccc gccgccgatc atcagctcgg ggtacggctt ctgcaccggc ttgggcatga
    10261 gcggcgcggc ttcgagctgg tagtacttgc ccttgtagtc ggccttggtc ccggtccaca
    10321 gcgccttcat cacgtgacag gcctcgtcga gacgggcgag ccgctcgcgg gtggtgtaga
    10381 agtccatccc gtaggcgcgg tgctcgttct cctgccagcc cgccccgacg ccgcagatga
    10441 ggcggccgcg cgagatgatg tccacgttgg tcgccatctt ggcgagcagc gcgggatggc
    10501 gataggtatt gccgctcacg atggtgccca cgcgcaggcg cgtggtcagg ccggccagcg
    10561 cggcgagcgt cgtccagcac tcgagcgtgt cgccgacccg gtcggccgtg ttgggcatga
    10621 agtgatcggt cacgcaggcc gcgtcccatc cggtctggtc tccgtggcgc cagagcgcga
    10681 gcacttcatc ccaggtcgtg ccggtcatgc cggtaaacaa ggcgaatttc atggtggctg
    10741 gatgctacgg gccgccactt gggaggtcaa gtggaaaccg gattcctagt catccacccg
    10801 ggcgcgctgg gcgacgtgct ccaggcggtg cccgcgctgc gcggcctgcg cgcggcggcg
    10861 cccgcggcgc cgatcacctt cgtgggccag ccgcggctcg cgcgcctgct cgtcgacctg
    10921 ggggcggcgc agcacgcgcg cacgttcgac ggattcggcc tcgaggccct cttcgtggac
    10981 gcgccgacac cgcccgcgct ggcggaagcg gtcagccgtg tgaagaacgt ggtctcgtgg
    11041 ttcggctcgc gcgacgagac gtaccgccgc cggctccgcg cgctcgcccc gcgcgcgctc
    11101 gtcgcgccgc cggtgcccga cgacgacacg ccggtctggc ggcacctgct ggggacgctg
    11161 gccggctggg atctcgcgat cccggagcgc gtggagccgc tgcgcgcccc tcccctgccg
    11221 gccagcgacg ccgctgcgga cgccggccgg ccccggctcg tcgtgcatcc cgggagcggc
    11281 ggcgactgga agcggtggcc ggccgagcgc ttcgccgagg tgatccgcgc gctacgccgc
    11341 cgtcgcgcat tcgaggtcct ggtgcatcag ggccccgcgg acgcggagcc cgcgcagcgc
    11401 ctgctgacgc tgctcgaggg cgacgctgcc gtcttgctcc agcccgagct gacgcgcctc
    11461 gccgcggtgc tcggcgccgc ccgcgcctac ctgggcggcg attcgggcgt gagccacctg
    11521 gccgccgcgg tgggcacccc gagcgtcgtg ctgtttccgt ccgcgacgta ccgacgctgg
    11581 acgccctgga gcccgaccgc gataccggtc gagatccagg gcatggacgg cgacgtcgcg
    11641 cgggcgctcg aggcgctcgc gctcgtgctc gcctgactcc tgcggctcgg cgtacgacta
    11701 tttctgcttg gcgacgacgt ggtccttgat cttgtcgtag gtggcctgcg gcacgagctt
    11761 cttctgcacc agctcgtcct tgcgcttgta gggccgattc tggacgatct tcttggcgta
    11821 ggcgtcgccg atgcccggga tcgtcttgag atcggcctcg ctcgcggtgt tgaggtccag
    11881 cgggtcgtgg cccgtggcct tcatctcgct cttggccggg ggcggcgcgg tcttgggggc
    11941 ctgggcccag gccgcggggg cgctgagcac gccggccgag aacaatgcca cgacgagtag
    12001 cgcgatcgat cgcttcatga ggtaccctcc tcctgtcgga tgaatgcgtg agctcctagc
    12061 gaatccccag cagctccacc tcgaagatga gggtcgcccc gggcgggatg accccaccgg
    12121 ccccgcggtc accgtaaccc aggtgcgcgg ggatgatcag cttgcgcttg ccgcccacct
    12181 tcatggagat gacgccctcg tcccagccct tgatgacgcg gcccttgccc accgggaact
    12241 cgaacggagt cccgcggtcc accgagctgt cgaacttggt gccgctcttg agccatccgg
    12301 tgtagtgcac gctcacggtg tcgccggtct tgggccgggc gccggctccc tcgaccaggt
    12361 ccacgtagcc cagcccggtc gcgctctcga cgacctgctc gtccttgttg tcggccatgc
    12421 ggtgcgctcc tttcgagggt caatagtacc agcggtggag ccgcgcgggg gccacgccgc
    12481 agaagtgcag gaagcggagg gcccacatga ggccgatcgt ccgcagcggc ccctcccgct
    12541 cccacttccg cgcggaggtg gtgacgcgca ggcggagcgc gcgcatccgc ccgcggcgct
    12601 tcagccggcg gctcagctcg atgtcctcca tcagcgggat ctcggggtag ccgccgatcg
    12661 cctcgaagtc ggcgcggcgc acgaagatgg cctgatcgcc ggtgcagatg cccgaggccc
    12721 gcgagcgcgt gttcatgcac caggcgatcg tgcgaaagag cgcgccctcg ttgtcgaagc
    12781 gcacgtcgaa gcggccgccc accacgcccg gcgcggcgag cgcctgctcg atcgcccggg
    12841 cggcgccgtc gggcaggcgc gtgtcggcgt gcaggaacag cagcgtgtcg ccggccgccg
    12901 cgcgcgcgcc gtggttcatc tggagcgccc ggccgcgcgc gctcgcgagg agccggggac
    12961 ccggctcgcg cgtgaccacc gccgcggtcc cgtcgcggct gccgccgtcc accacgacga
    13021 tctccgcgcc cgggcaatcg cgcgcgagat cgggcaggag ccgcgcgaga tgcgccgcct
    13081 cgttcagcgc ggggatgacg atggcgagcc tcatcggccc ggcccgatgg tcagcgcgtc
    13141 gtaataggcg acggcccgct cgccggtatc gtcggtgtcg gtcatcagcg cgatgcccac
    13201 gatgcggggc ggctccccgc cgactatccg tcggaagtct tcatagatat cccgggtctc
    13261 ggtcacccag cgccccacct ccgcggcccc actgcgcgcc accaggatcc gggcgcgcgc
    13321 ggtgtaggcg ttgtcgagca ccgtgcccac cggcagccgg ctctcccaca cgtagttgag
    13381 ggcgaggccg ggcgggtagc ggccgtacag gacccggtac gccccgaagc gcgcgcgctc
    13441 ccaccgcgag gcggcggccg gatcgtaccg gtacgccacg tacacgcgcg ccacgtagtc
    13501 gtcgccctgc ttgcggcgcg ggtccgactt cgcgagcacg ccctccacct tccagcgcca
    13561 ggagagcacc cggtagacct tcggatcgag gtccagcggg tggaagagcc cggcggccgc
    13621 cgcgtcgctc tccgcgcgca gcacccaccg ctcgccctcg agcaccaccg tgtagcgcgt
    13681 gtggcgcgac acgcgcggga aggtcagcgg ctcccagccc ggcggcggcg cggtcgcatc
    13741 cgtgggcggg gcgaagtcgc cgagccggag cggctcggcc gcgcggccgg cggcggcggc
    13801 gagcagcagc ccgagcagcg cggcgcccac gcgcccgagg cccgcgcgcg gcgtcctcat
    13861 cccgtgcgca gcaggtcgtc gagcgcggcg ctgcgcccgc gcagccaccg gggcagcagc
    13921 gagagcgcca cgagcagcac gccggccatc gccagcagga gcagcatgcg ccgggcgtcc
    13981 caggcgcccg ccaccaccgc ccccgccgcc gcggtgaagg ccagcgtgcc cggcagcatg
    14041 cagaggaacg aggtcagcgc gtacgcgccg aagccgatgg aggtgagccc gtaggcgtag
    14101 ttctgcaggt tgaacgggaa gatcggcacc agccgcgtga tcatcacgat gcgaaagccg
    14161 tggcgcgcca ccgcgcggtc catgcgctcg agcgcgggat agccggccag ccagcgcgcg
    14221 accgccgcgc gggccgcgta gcgcgcgatc aggaacgcca ggcaggcgcc ggtggtggcc
    14281 gcgatcgcca cgtagaccgt gccccacgcc gtgccgaagg ccaggccccc caggatggtg
    14341 agcggcagcg cgggcacgaa ggcgaccacc gcgaccacgt agcccaggat gaagaccacc
    14401 ggcgcccacg cgcccaggcc gtcgatccgc tcgcgcagcc gggccaggtt ctcgagccgg
    14461 accaggtcgg cgagcccgag gacgcggacg agcaccaccg ccagcgcgag ggcgagcagc
    14521 gtgacgacca gcagccgccg gctcagtact tgccccgggc gcgctcgcgg gcggggaggt
    14581 tctcgaggca ggtggtccac cacccggtgc ggatcgtctc cgcgaactcg ggcggcagcc
    14641 cctcccggtc catctcgcgg gccagcgtct cgtgatcgta ggccagcggc acgaactcca
    14701 cctgcagatc ggggcccgcg gtcagcaccg tgtaccacac gttggtccgg ccgtcgttct
    14761 ccggccgccc gatcaccccc acgttgaccg cgtggcgggc gtccggcagc gcgcggtgcc
    14821 acttgatccc ggtgtgggtg cacagcatga cgtcggcctc cgcacgctcc atcaggtggc
    14881 gcaggagccc gttcggggtg gccgactccc acaggaactc g
//
LOCUS       DASUFA010000044         9108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_811259, whole genome shotgun sequence.
ACCESSION   DASUFA010000044 DASUFA010000000
VERSION     DASUFA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811259"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFX87_01665"
     CDS             <1..249
                     /locus_tag="VFX87_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEX4993638.1"
                     /translation="SCYLPLAGTLGTEVSPDNRDVPIFWAHGAHDPMLPLAMAEQGRA
                     QLGELGYQIDWHQYPIPHSVSAEEIADVARWLGRVLAA"
     gene            274..894
                     /locus_tag="VFX87_01670"
     CDS             274..894
                     /locus_tag="VFX87_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4993639.1"
                     /translation="MQDRLTMPIGNAIFTQRSTRKLRPDPIPVEDLHLLMEAAVKAPN
                     GGNNQVARFLIVNDRAKIREFGALYKEAWWAKRWDDHKWTKPEDIPLTDKNHRSAMGL
                     SEDMKDVPCVVFAFTAPGGGANSVIPACQNLMLAAHALGIGSLPTTLHAKVMDRFHAM
                     FDIPKEMTFHFCIPLGYPAVPYGPSKRKPTAETTFLNRWGGAVPWR"
     gene            885..1799
                     /locus_tag="VFX87_01675"
     CDS             885..1799
                     /locus_tag="VFX87_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993640.1"
                     /translation="MAVTRKPAVSLAAVPGRRKATVEMAKRLEEEGFSGLYCPSLGDG
                     LGLCEAIALATRSIPFGTSISNIYTRHPLDFAQTAALIHELSGGRFRFGVGISHGPTH
                     DRLGIKPGKPLEDMRRFVQELKSGAKLAGELPPITLATLRKRMTALSAEIAQGAVWAN
                     AARSHMPESLKALPDAARTGPEFFIGNMVPTCIDTDRAAAAAVNRRTLTGYVKLPNYQ
                     NYWIEAGFEEEMEAIRRAITAGQEDKLPSLMSDRWLSQVTLYGSPTEVRDGVEAWYAA
                     GVKTLIVVPSSAKGNQMTALQELIDLYR"
     gene            1897..2172
                     /locus_tag="VFX87_01680"
     CDS             1897..2172
                     /locus_tag="VFX87_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993641.1"
                     /translation="MNDLELSHPHALDVQLVDLQPANSRPADEQPTDPEATDRESAHG
                     QRTERQRSDRQRTHRAHANGGCRRRPRADIATFCIAIRNGSRAFPPG"
     gene            complement(2233..3357)
                     /locus_tag="VFX87_01685"
     CDS             complement(2233..3357)
                     /locus_tag="VFX87_01685"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4993642.1"
                     /translation="MSYDAPFAGLKVVDLSQGIAGPYSAMLLAQYGADVIKVEPPEGD
                     WARELGKRYGDLSAFFVAANLGKRAIVLDLKAEEGQATLRRLLSTADVFLESYRPGVV
                     ARLGIAYPDVSALNPRIIYLSVSGFGQTGPDAERPAVDTVFQAFTGLMSVNRGLDGIP
                     HRVPVIVMDMATALSNFQAMAVALYARRGEPRGRYIESSLLRGGATIQAVSMIQHHLE
                     GGKPQPGLTPSGTFKASDGWINVTILRDAEFPALCDALELPAAGKEPRFATNNLRFAN
                     VAALSALLEPAFARRTTADLSARLRAARLMHQRVNTYLEFLDDPQVAAAGAVAWIEQP
                     GLGRVPVPQVPGLPLIRSGTPLAMAPSLDQHRKEILAELD"
     gene            3429..3887
                     /locus_tag="VFX87_01690"
     CDS             3429..3887
                     /locus_tag="VFX87_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4993643.1"
                     /translation="MLKVEKPQDLLQHVGKELGPSEWLTVTQEMIDKFAEATGDHQWI
                     HVDVERAKKEMPGGKTIAHGYLTLSLLPQLAPTLMKVEKRRRGLNYGSNRVRFTAPVP
                     AGARVRLRQKLVKVEPVEDNGFRITSEMTMEVEGNPRPAMVAETLGIVYG"
     gene            complement(4000..4572)
                     /locus_tag="VFX87_01695"
     CDS             complement(4000..4572)
                     /locus_tag="VFX87_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4993644.1"
                     /translation="MMKPEEVLKLIQAARGTAICVPTMTTAPAWRKIAPDDLSVGCVG
                     FMGGASAMGLGLALGAPDRRVIVFDGDGSLLMQLGSLATIAGAAPRNFTHLLFKNGVY
                     HTSGSQETPGGAGTDFAAMAKAAGYRNAYTVTTLEEFKKRLPGFLTEDGPIMVQLITG
                     LAESTPMTAGPSTPFHQQVAELRAKVTGAR"
     gene            complement(4572..5111)
                     /locus_tag="VFX87_01700"
     CDS             complement(4572..5111)
                     /locus_tag="VFX87_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993645.1"
                     /translation="MPESVPSSLIIDAFKEIFQSERRGAKRQDPLGWIISVPDTHQKS
                     VLAALDKERSLRVLTCSTEDDANAVAAGLYMTGEPVIVMIQHAGLYASVNTIRGISMD
                     GKIPIFYMIGLLSREKDKDPKDSRHSMVRFAEPLLDVFSIPHARMEGPDDVKLIPEYY
                     RKSRERKGPAAVLVGLETA"
     gene            5158..5478
                     /locus_tag="VFX87_01705"
     CDS             5158..5478
                     /locus_tag="VFX87_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993646.1"
                     /translation="MTPPLPDLLRDQDGRHPFWLRVIFMGIAVVCFALGVVGWLIPVM
                     TGVPFYVAGLVFLGLASDRTRRFINRTEHRLSENTRRRIRRVIAKIPGRWVRSLVNIP
                     EGAF"
     gene            5497..6561
                     /locus_tag="VFX87_01710"
     CDS             5497..6561
                     /locus_tag="VFX87_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993647.1"
                     /translation="MLALLSRAAALACLLCLPLPAGGADAPSPDDGQTLDVRRPSPEQ
                     RRRLLAGDTIGYQVAETNETELGAGVAMYLPVPLARVAEALTSPDIVLKEPSITAWGP
                     LASDATVAALAGYRLSAGEVAEAQDAFDAVAGGRLNLSAAEIEAFRAAKATLRGADRA
                     AVAESGAAQWRALLLQRVQAFQARWLDGVAPYARRGGVTDPAAVLRVAAGDARIVAHL
                     MPRLADALLRFPAEQSRTAISQFYWVKRQVQGRADPILIHHLVDVTPQLALYVERHVY
                     VGHSYNASQILSGAVPYEDGVLVFSSNRVSTDQVAGLGGELKRAIGRRQLRGEIVKRF
                     DRIRAALLKPAAPPERVESP"
     gene            complement(6562..7365)
                     /locus_tag="VFX87_01715"
     CDS             complement(6562..7365)
                     /locus_tag="VFX87_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4993648.1"
                     /translation="MSWLAAALEWQSRAVVARHLRVYMRNWHTAFLPPAVEPVTMLLA
                     FGVGLGGYVATLTWQGRPIEYMSYVAPGLLAYATFMTAIFQSLFAAFIRMRYQRTWEG
                     QLTTQVALPHVVWGEVLWAGLLATMYVVIVSAVLAALHAAGFLHIRIALLPVLLPIVF
                     VAGCGFAALGLCFTAIVPTIDHMNLPVFLLVLPMGLLSSTYFPLEHPLLVALNVVNPL
                     HHLAQAFRGILLGGPISGHLAAALILSGLLLGILVPLDLRLLRRRVLGD"
     gene            complement(7362..8264)
                     /locus_tag="VFX87_01720"
     CDS             complement(7362..8264)
                     /locus_tag="VFX87_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4993649.1"
                     /translation="MSVLLDARNLVKYLGGRRVVDGVDLACESSQIVGLLGPNGAGKT
                     TTLRMLYGFLHPDEGRIHVDGVELGRDLVRAKRSIGVCTQDDTFDGDFTVEQNLTIAA
                     TYFRPRPTDLTRRVGELLERFELDPYAGQKPETLSGGYRRRLMIARALVHRPRLLFLD
                     EPTTGLDPQARMGVWDLVDGLRAEGLGIILTTHYMDEAERLSDALTVLARGRVVARGT
                     AKTVLGDLVGEHVIVVDVSQGGAVAAWLTARGRGEPASVLGAWHVPVSGEELALFARA
                     FPALRYEVRGPTLDDLFLKLSLTP"
     gene            8350..8814
                     /locus_tag="VFX87_01725"
     CDS             8350..8814
                     /locus_tag="VFX87_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993650.1"
                     /translation="MKLKRPRKSPRSKRPASKRAGHTRARAPHDPLLARMAGADHREV
                     GGVSIDTTRAANGRVKRVCYPPGFRWSTHMKPLVNTPLCMHAHVGFLARGRVRGTYAD
                     GCAFDLAAPRAVVIEPGHDAWVVGKDAAVLIEFDTESSTAARFGLPDSHRHA"
     gene            8831..>9108
                     /locus_tag="VFX87_01730"
     CDS             8831..>9108
                     /locus_tag="VFX87_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX4993651.1"
                     /translation="MTSELEDKFQTLHEIVRAARMNLAPGPWDYLVGGTETETTLRRN
                     RLGIDSLAFRPRVLRDVSRIDPGSKFFGKKVRIPVMLAPIGSIESFTPG"
BASE COUNT         1431 a         3291 c         3102 g         1284 t
ORIGIN      
        1 tcgtgctatc tacccctcgc cggcacgctg ggcaccgagg tgtcgcccga caaccgggac
       61 gtgccgatct tctgggcgca cggcgcccac gatccgatgc tcccgctggc gatggccgag
      121 caggggcgcg cgcagctcgg cgagctcggc taccagatcg actggcacca gtacccgatt
      181 ccgcactccg tctcggcgga agagatcgcc gacgtcgccc gctggctcgg gcgcgtgctc
      241 gccgcgtgac cgccaccctc aggaggtaag cccatgcagg accgtctgac catgcccatc
      301 gggaacgcga tcttcaccca gcggtctact cgaaagctcc gccccgatcc gatcccggtc
      361 gaggatctgc acctgctgat ggaggcggcg gtgaaggccc ccaacggcgg caacaaccag
      421 gtcgcccgct tcctgatcgt gaacgaccgc gcgaagatcc gcgagttcgg cgcgctctac
      481 aaggaagcgt ggtgggccaa gcgttgggac gaccacaagt ggacgaagcc ggaggacatc
      541 ccgctcaccg acaagaacca ccgctcggcg atgggcctgt ccgaggacat gaaggacgtg
      601 ccctgtgttg tattcgcctt caccgccccg ggcggcggtg ccaactcggt gatcccggcc
      661 tgccagaacc tcatgctggc cgcgcacgcc ctcggcatcg gctcgctgcc caccacgctc
      721 cacgccaagg tgatggaccg cttccacgcg atgttcgaca tccccaagga gatgaccttc
      781 cacttctgca tcccgctcgg gtatccggcc gtcccgtacg ggccgagcaa gcggaagccg
      841 accgcggaga ccaccttcct caatcgctgg ggcggagccg tcccgtggcg gtgacgcgca
      901 agcccgcggt cagcctcgcc gccgtccccg gccgccgcaa ggccacggtg gagatggcga
      961 agcgcctcga ggaggagggc ttcagcgggc tctattgccc gagcctgggc gacgggctcg
     1021 gcctctgcga ggccatcgcg ctcgccaccc gcagcatccc gttcggcacc agcatctcca
     1081 acatctacac gcgccaccct ctcgacttcg cccagaccgc cgcgctcatc cacgagctgt
     1141 ccggcggccg tttccgcttc ggcgtcggca tcagccacgg cccgacgcac gaccgcctcg
     1201 gcatcaagcc gggcaagccg ctcgaggaca tgcgccgatt cgtgcaggag ctgaaatcgg
     1261 gcgccaagct cgccggcgag ctgccgccga tcacactcgc gacgctccgg aagaggatga
     1321 ccgcactctc cgccgagatc gcccagggcg cggtgtgggc caacgcggcg cgctcgcaca
     1381 tgccggagtc gctcaaggcc ctgcccgacg ccgcgcgcac cgggcccgag ttcttcatcg
     1441 gcaacatggt gccgacctgc atcgacaccg accgcgccgc cgccgccgcg gtgaaccgcc
     1501 gcaccctgac cggctacgtc aagctgccga actaccagaa ctactggatc gaggccggct
     1561 tcgaggagga gatggaggcg atccgccgag ccatcacggc gggccaggag gacaagctcc
     1621 cgtcgctgat gtccgaccgc tggctctccc aggtcacgct ctacggcagc ccgacagagg
     1681 tgcgcgacgg cgtcgaggcc tggtacgcgg cgggggtgaa gacgctcatc gtggtgcctt
     1741 cctccgcgaa gggcaaccag atgacggccc tacaggagct gatcgacctc taccgctgac
     1801 tccggtgctc gagggagtcc ggccggcaac cgcctggaca aggattgcga ggcgctagcc
     1861 actcccaggc gtgttgtcgc cccacgtcgc cggcccgtga acgatctgga gctctctcac
     1921 ccgcatgcgc ttgacgtcca gctcgtcgat ctccagcctg cgaattcgcg acctgccgat
     1981 gaacaaccca ccgatcccga ggcgaccgat cgcgagagcg cccacggcca gcgcaccgaa
     2041 cgccagcgct ccgatcgcca acgcacgcac cgtgcgcacg ccaacggcgg ttgtcgccgc
     2101 cggccacgtg ctgacatcgc cacgttctgt atcgcgatcc gcaatggatc gcgcgccttc
     2161 cctcccggct agccggtccg aattcattct tcctccttcc aacgcgacgg tgccccctcg
     2221 gccaaggtcg ccctagtcca gctcggcgag gatctccttg cggtgctgat cgagcgaggg
     2281 cgccatcgca agcggcgtcc cgctgcggat cagcggcagg cccggcacct ggggcaccgg
     2341 cacccggccg agccccggct gctcgatcca ggccaccgcg ccggcggccg ccacctgcgg
     2401 gtcatcgagg aattcgaggt acgtgttcac ccgctgatgc atgagccgcg ccgcccgcag
     2461 ccgcgccgaa aggtccgctg tggtgcgccg ggcgaatgcg ggctcgagca gcgcgctgag
     2521 cgcggccacg ttggcaaagc ggaggttgtt ggtggcgaag cgaggctcct tcccggccgc
     2581 gggaagctcg agcgcgtcgc agagcgcggg gaactccgcg tcgcgcagga tcgtcacgtt
     2641 gatccagccg tcactcgcct tgaaggtgcc cgacggggtg aggcccggct gcggcttccc
     2701 gccctccaga tggtgctgga tcatcgagac cgcctggatc gtcgctccgc cgcgcagcag
     2761 gctcgactcg atgtagcggc cgcgcggctc gccgcggcgg gcgtagagcg cgaccgccat
     2821 cgcctggaag ttgctgagtg cggtggccat gtccatcacg atcacgggga cacggtgcgg
     2881 gatgccgtcg agcccgcggt tcaccgacat gaggccggtg aaggcctgga acacggtgtc
     2941 caccgccggc cgctccgcgt cggggccggt ctgaccgaaa ccggagacgg acagatagat
     3001 gatgcgcggg ttcagcgccg acacgtccgg ataggcgata ccgagccggg cgaccacccc
     3061 gggccgataa ctctcgagga agacgtccgc ggtggagagg agccggcgca gcgtggcctg
     3121 gccctcctcg gccttgaggt cgagcacgat cgcgcgcttg ccgagattgg ccgcaacgaa
     3181 gaacgcgctc aggtcgccgt accgcttgcc gagctcgcgc gcccagtcgc cctccggcgg
     3241 ctccaccttg atgacgtcgg caccgtactg ggccagcagc atggccgagt agggccccgc
     3301 gatcccctgg ctcaggtcca ccaccttgag gccggcgaag ggcgcgtcgt aactcatcgc
     3361 gtgctttcct ccgggactgg gactgggcga taatggcgcg cgcagatcat acccgaggga
     3421 ggaacgctat gctcaaggtc gagaagcccc aggatttgct ccagcacgtg ggcaaggagc
     3481 tgggtccgtc cgagtggctc accgtgaccc aggagatgat cgacaagttc gcggaggcca
     3541 ccggcgatca ccagtggatc cacgtggacg tggagcgggc caagaaggag atgccgggcg
     3601 gcaagaccat cgcccacggc tatctcacgc tctcgctcct cccgcagctc gccccgacct
     3661 tgatgaaggt cgagaaacgg cgccgcgggc tgaactacgg ctcgaaccgc gtgcgcttca
     3721 ccgccccggt gccggcgggc gcccgcgtcc gtctgcgcca gaagctggtc aaggtggagc
     3781 ccgtggagga caacgggttc cgcatcacca gcgagatgac catggaggtc gagggcaacc
     3841 cgcgccccgc catggtcgcg gagacgctcg gcatcgtcta cgggtagtca tcggaggggc
     3901 gccttctgaa gtccctgaag ggggccccct ccgaacctcc cccccagaac ggctgcgccg
     3961 gcggagccgg cgctcgaaca gagtccctcg gacccgccgc tagcgcgcgc cggtcacctt
     4021 cgcgcgcagc tcggccacct gctggtggaa gggcgtgctc ggccccgcgg tcatcggcgt
     4081 gctctccgcc agcccggtaa tgagctgcac catgatcggg ccgtcctcgg tgaggaagcc
     4141 gggcaggcgc ttcttgaact cctccagcgt ggtgacggtg taggcgttgc ggtagcccgc
     4201 ggccttggcc atcgcggcga agtcggtccc cgccccaccc ggcgtctcct gcgagccgga
     4261 ggtgtggtag acgccgttct tgaagagcag gtgcgtgaag ttacgcgggg ccgcgcccgc
     4321 gatcgtggcg agcgagccga gctgcatcag cagcgagccg tcgccgtcga agacgatcac
     4381 ccgacggtcg ggcgcgccca gggccaagcc gaggcccatg gcggaggcgc cgcccatgaa
     4441 gcccacgcag ccgacggaaa gatcgtcggg cgcgatcttg cgccaggcgg gcgcggtggt
     4501 catggtcggc acgcagatgg ccgtcccgcg ggctgcctgg atcagcttga gaacctcctc
     4561 cggcttcatc atcacgccgt ctccaggccg acgagcaccg cggcggggcc cttccgctcg
     4621 cggctcttcc ggtagtactc ggggatcagc ttcacgtcgt ccgggccttc catgcgggcg
     4681 tgggggatgc tgaacacgtc gagcagcggc tcggcgaagc ggaccatgga atgccgcgag
     4741 tccttcgggt ccttgtcttt ctcgcggctg agcagcccga tcatgtagaa gatcgggatc
     4801 ttgccgtcca tcgagatgcc gcggatcgtg ttcacgctgg catacaggcc ggcgtgctgg
     4861 atcatcacga tcaccggctc gccggtcatg tagagccccg cggccaccgc gttggcgtcg
     4921 tcctcggtcg agcacgtgag gacgcgcagg cttcgctcct tgtcgagcgc ggccagcacg
     4981 ctcttctggt gcgtgtcggg cacgctgatg atccagccga gcggatcctg gcgcttggcg
     5041 ccccggcgct ccgactggaa aatctccttg aatgcatcga tgatgagaga ggacggtacg
     5101 gactcgggca ttggctcctc cttggcgcgg cgtatggtac accagatcgg gcggcgcatg
     5161 acacccccgc tgccggacct cctccgggac caggacgggc gccacccgtt ctggctgcgt
     5221 gtcatcttca tgggcatcgc ggtcgtgtgc ttcgcgctgg gcgtcgtcgg ctggctcatc
     5281 ccggtgatga cgggcgtccc gttctacgtg gccgggctgg tcttcctggg cctggccagc
     5341 gaccggaccc gccgcttcat caaccgcacg gagcaccggc tctcggagaa cacccggcgg
     5401 aggatccggc gggtgatcgc caagatcccg gggaggtggg tgcgcagcct cgtcaatatt
     5461 cccgagggtg ccttctgagc cgcctaagat aggagcatgc tcgcactatt gagcagagcg
     5521 gccgccctcg cctgcctgct ctgcctgccg ctgcccgccg gaggcgccga tgccccgtcg
     5581 ccggacgacg gccagaccct cgacgtccgc cgcccctctc cggagcagcg ccgccgcctc
     5641 ctggccggcg acaccatcgg ctaccaggtg gccgagacca acgagaccga gctgggcgcg
     5701 ggggtcgcca tgtacctccc ggttccgttg gcccgtgtgg ccgaggccct gacgagcccg
     5761 gacatcgtgc tgaaggagcc gagcatcacc gcgtggggac cgctggcctc cgatgcgacg
     5821 gtggccgcgc tcgcaggcta caggctctcg gccggtgagg tcgcggaggc gcaggatgcc
     5881 ttcgacgcgg tggccggcgg acgcctcaat ctctcggcgg ccgagatcga ggccttccgc
     5941 gcggcgaagg ccacgctgcg cggcgccgac cgggcggcgg tggccgagag cggcgccgcc
     6001 cagtggcggg cgttgctcct ccagcgcgtg caggcgttcc aggcgcgctg gctcgacggc
     6061 gtggcgccct acgcgcggcg cgggggcgtc accgatccgg ccgcggtgct gcgggtggcc
     6121 gcgggggacg cgcggatcgt cgcccacctt atgccgcggc tggccgacgc gctgctgcgc
     6181 ttcccggccg agcagagccg caccgcgatc agccagttct actgggtgaa gcgccaggtc
     6241 cagggccggg ccgacccgat cctgatccac catctcgtgg acgtgacgcc ccagctcgcg
     6301 ctctacgtcg agcgccacgt ctacgtgggc cactcctaca acgcgagcca gatcctctcc
     6361 ggcgcggtgc cgtacgaaga cggcgtgctc gtcttctcga gcaatcgcgt ctccaccgac
     6421 caggtcgcgg gcctcggcgg ggagctgaag cgcgcgatcg gccggcgcca gctccgcggc
     6481 gagatcgtca agcgcttcga tcggatccgc gccgcgctgc tgaagccggc ggcgccgccc
     6541 gagcgcgtgg agagcccgta gctagtcgcc gagcacccgc cgccgcagga ggcggagatc
     6601 gagcggcacc aggatcccga gcagcaggcc cgagaggatc agcgccgccg cgaggtgccc
     6661 cgagatcggc cccccgagca ggatgccgcg aaaggcctgg gccagatggt ggagcgggtt
     6721 caccacgttg agcgcgacca gcagcgggtg ctcgagcggg aagtaggtgg acgagagcag
     6781 ccccatcggc agcacgagca ggaacacggg caagttcatg tggtcgatcg tcggcacgat
     6841 cgcggtgaag cagagcccga gcgcggcgaa cccgcagccc gccacgaaca cgatgggcag
     6901 cagcacgggc agcagcgcga tccggatgtg gaggaagccc gccgcgtgga gggccgcgag
     6961 caccgccgac acgatcacca cgtacatggt ggcgagtagc cccgcccaca gcacctcgcc
     7021 ccagaccacg tgcggcagcg cgacctgggt ggtgagctgg ccctcccacg tgcgctggta
     7081 gcgcatgcgg atgaacgcgg cgaagagcga ctggaagatc gcggtcatga aggtcgcgta
     7141 ggccagcagc cccggggcca cgtagctcat gtactcgatc gggcggcctt gccaggtcag
     7201 cgtcgccacg tagccgccga gccccacccc gaaggccagc agcatggtca ccggctccac
     7261 cgcgggcggc aggaatgcgg tgtgccagtt ccgcatgtag acgcgcaggt gccgcgccac
     7321 caccgcgcgg ctctgccact cgagggccgc ggccagccag ctcatggcgt gagcgagagc
     7381 ttcaggaaga gatcgtcgag ggtggggccg cgcacctcgt agcgcagcgc ggggaacgcg
     7441 cgcgcgaaca gggccagctc ctcgcccgac accggcacgt gccaggcccc cagcacgctc
     7501 gccggctcgc cgcggccccg cgcggtgagc caggccgcga ccgccccgcc ctggctcacg
     7561 tccaccacga tgacgtgctc gcccaccagg tcgcccagca cggtcttggc ggtcccgcgc
     7621 gccaccacgc ggccgcgggc cagcacggtg agcgcgtcgc tcagccgctc cgcctcgtcc
     7681 atgtagtgcg tggtgaggat gatgccgagc ccctcggcgc gcagcccgtc cacgagatcc
     7741 cacaccccca tccgcgcctg gggatcgagg ccggtcgtcg gctcgtcgag gaacagcagg
     7801 cgcggccggt gcacgagcgc gcgcgcgatc atgagccgcc gccggtagcc gcccgagagc
     7861 gtctccggct tctggcccgc gtaggggtcc agctcgaagc gctcgagcag ctcgcccacg
     7921 cgccgcgtca ggtcggtggg ccgcgggcgg aagtaggtcg ccgcgatcgt gagattctgc
     7981 tccaccgtga agtcgccgtc gaaggtgtcg tcctgggtgc acacgccgat ggagcgcttg
     8041 gcccgcacca gatcgcggcc cagctccacg ccgtccacgt ggatgcggcc ctcgtcgggg
     8101 tgcaggaacc cgtacagcat ccggagggtc gtggtcttcc ccgctccgtt gggcccgagc
     8161 aggcccacga tctgcgagga ctcgcaggcg aggtccaccc cgtccaccac ccggcggccg
     8221 cccagatact tgaccagatt gcgcgcgtcc agcagcaccg acatagctct cgattgtacc
     8281 cgggcttgac aagcctggcg cgcggagtca gtgtctcgaa ggtccaagaa cgccaggagg
     8341 aatcgcccca tgaagctcaa gcgcccgcgg aagtcacccc gctccaagcg ccccgcttcg
     8401 aagcgcgccg gtcacacgcg ggcacgcgcc ccgcacgatc cgctgctggc ccgcatggcg
     8461 ggcgccgacc atcgcgaggt gggcggtgtg tccatcgaca ccacccgcgc cgccaacggc
     8521 cgcgtgaagc gcgtgtgcta cccgcccggc ttccgctggt ccacccacat gaagcccctc
     8581 gtgaacacgc cgctctgcat gcacgcccac gtcgggttcc tggcgcgcgg tcgcgtgcgc
     8641 ggcacctacg ccgacggctg cgccttcgac ctcgccgcgc cgcgcgcggt ggtgatcgag
     8701 cccggccacg acgcgtgggt ggtcggcaag gacgcggccg tgctcatcga gttcgacacc
     8761 gagagcagca ccgcggcccg cttcgggctg cccgacagtc accgccacgc gtgaatccga
     8821 gagagagccc atgacctccg agctcgaaga caagttccag acgctgcacg agatcgtgcg
     8881 cgccgcccgc atgaacctgg cccccggtcc ctgggactac ctggtgggcg gcaccgagac
     8941 cgagaccacg ctgcgccgca accggctcgg catcgacagc ctcgcgttcc ggccgcgcgt
     9001 gctgcgcgac gtctcgcgca tcgacccggg atcaaagttc ttcggtaaga aagtccgcat
     9061 cccggtgatg ctggcgccca tcggctccat cgagtccttc acgccggg
//
LOCUS       DASUFA010000045         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1040836, whole genome shotgun sequence.
ACCESSION   DASUFA010000045 DASUFA010000000
VERSION     DASUFA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040836"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFX87_01735"
     CDS             <1..760
                     /locus_tag="VFX87_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993652.1"
                     /translation="SLLLRPPGRGLVEPAPASPGPLDSGMTGFVIVMLATVLFDGLLG
                     TRAWRALDRALSAGLARVTDREGYLLATFGLLAVWLVLLGAYLLTARLTARLAGAGSA
                     AATARLLAPSLVPIATAYNLAHNSSYLLLQGQELIPLLSDPLGRGWDLLGTATWSPDV
                     TLVGSRFAWYAAVGAIVTGHVISVWLAHRIALREWSPPGLALRASLPLTALMVVYTAL
                     SLWVIADPLVRFREPDPSYSRLLIPAAPMPRTPG"
     gene            complement(738..917)
                     /locus_tag="VFX87_01740"
     CDS             complement(738..917)
                     /locus_tag="VFX87_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015199851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEX4993653.1"
                     /translation="MTYGCAFCGEDNDLTVDPSAGRRQTFTEDCTVCCRPNLITLVLD
                     EDGDVSIEVTQEYEA"
     gene            1075..1416
                     /locus_tag="VFX87_01745"
     CDS             1075..1416
                     /locus_tag="VFX87_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX4993654.1"
                     /translation="MAHKLFIGGLAFSTSTDRLREVFAQTGVVESAAVVTDRDTGNSR
                     GFGFVEMATAEEAAEAVKQLNGKEVDGRTLKVELANSTGAGGGRSGGGGGGGYRSRGG
                     SGGSGSGGGRW"
     gene            complement(1438..>3284)
                     /locus_tag="VFX87_01750"
     CDS             complement(1438..>3284)
                     /locus_tag="VFX87_01750"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX4993655.1"
                     /translation="VPPPAALEAWLVARRPLPAHAAYLEARYADEPYRLILALLAADL
                     EAAARDDMTARLLEATPHAARVRLTDLTRPLDLVASALPPALAHDRLWTVRRQIEAFG
                     LHAARLDIREESSRLAAALGELLSALGLGAGFAGEDDRPRTETLLRLLARPVGEIPAS
                     PALSPEAAETWAVFRLLARARAVYGPELLGPFIISMTRGPADLLAVLLLARWAGGDAG
                     LAIVPLFETLGDLDAAPRILDDLFGFPVYRDHLARAGGQQTVMIGYSDSNKDGGYLAA
                     NWALYRAQEEIVRVCAGRGVPLTLFHGRGGTVARGGGPAGRAIRAQPPGTVGGRFRLT
                     EQGEVLAARYGDPALAHRHLEQIVSAVLEASAPSVPGAEEPGAPPGAPMEWRRAMAAL
                     SGAARTSYRSLVESPGFVEFWRAVTPIDEISRLRLGSRPATRRGPTLEIAQVRAIPWV
                     FSWMQSRFNLPGWFGLGAALAEGGAPPELLRAMHRGWPFFRALIDNAEMSLLKADLGI
                     ATLYARLDPERVRAAALFARIEAEYGRAREAILAITGQRALMDGEPVIQRSIELRNPY
                     VDPLNYLQVEMLRRLRALPDPEGPEGEALREVIVLTVNGIAAGLRNTG"
BASE COUNT          429 a         1195 c         1200 g          460 t
ORIGIN      
        1 cagcctgctc ctgcggccgc cgggccgagg cctcgtcgag ccggcgcccg cgtcccccgg
       61 ccccctggat tccggaatga cgggctttgt catcgtcatg ctggccaccg tgctcttcga
      121 cggcctcctc ggcactcgcg catggcgggc gctggaccgt gccctgagcg cggggctcgc
      181 ccgcgtaacg gatcgcgaag gctatctcct ggccacgttc gggctgctgg cggtgtggct
      241 ggtccttctc ggcgcctatc tcctcacggc ccggctcacc gcccgcctcg ccggcgcggg
      301 ctcggcggcc gccaccgcgc gccttctggc gccgagcctg gtccccatcg cgaccgccta
      361 caatctcgcc cacaacagct cctacctctt gctgcagggc caggagctga tcccgctgct
      421 ctccgatccc ctcgggcgcg gctgggatct cctcggcacc gcgacctgga gcccggacgt
      481 cactctggtg ggctcgcgct tcgcctggta cgccgcggtg ggcgccatcg tcaccgggca
      541 tgtgatctcg gtgtggctgg cccatcggat cgccttgcgc gagtggagcc ctccggggct
      601 ggcgctgcgc gcgtcgcttc cgctcaccgc gctgatggtg gtctacacgg ccctgagcct
      661 ctgggtgatc gcggacccgc tggtgcgctt ccgggagccg gatccctcct actcccggct
      721 cctgattccg gcggcgccta tgcctcgtac tcctgggtga cctcgatcga gacgtcgccg
      781 tcctcgtcga ggaccagggt gatgaggttg gggcggcagc acaccgtaca gtcctcggtg
      841 aacgtctgcc gccggcccgc gctcgggtcc accgtcaggt cgttgtcctc cccgcagaag
      901 gcgcaaccgt aggtcacgtt tcgcatctta cgcgagcggg atgtgtcatg atggacccgt
      961 ctggaccggc cgccggttcc tcgtggaccc ccacctcccc agatcacgac tcccgagccg
     1021 tcttccgcgt tccggagcgt gtccctcatc gcgggagatc gaccggaggc aatcatggct
     1081 cacaagctct tcatcggagg tctcgcattt tccacctcga cggaccgtct gcgcgaggtg
     1141 ttcgcccaga ccggcgtcgt cgagtcggca gccgtcgtga ccgaccgtga caccggcaac
     1201 tcgcgcgggt tcggcttcgt cgagatggcg acggcggaag aggcggccga ggccgtcaag
     1261 cagctcaatg gcaaggaagt ggacggacgc acgctgaagg tcgagctggc caattccacc
     1321 ggcgcgggcg gaggccggag cggcggcggt ggcggtggcg ggtatcggag ccgcggcgga
     1381 agtggcggca gcggcagcgg cggcggtcgc tggtagcccg tctcctcggc ggctgcctca
     1441 gccggtattt cggagtcccg ccgcgatccc attcacggtg aggacgatca cctcgcggag
     1501 cgcctccccc tcgggcccct ccgggtccgg gagggcgcgc agccggcgta gcatctcgac
     1561 ctggagatag ttgagcgggt cgacgtaggg attccgcagc tcgatggagc gctggatgac
     1621 gggctcgccg tccatcagcg cgcgctgccc ggtgatggcg aggatggcct cgcgggcccg
     1681 cccgtactcc gcctcgatgc gggcgaagag cgcggccgct cggactcgct cgggatcgag
     1741 gcgcgcgtac agcgtggcga tgccgaggtc ggccttgagc agcgacatct ccgcattgtc
     1801 gatgagggcc cggaagaagg gccagccccg atgcatcgcg cgcaggagct cgggcggggc
     1861 gccgccctcc gccagcgcgg cgcccagccc gaaccagccg gggagattga agcggctctg
     1921 catccaggaa aagacccacg ggatggcgcg cacctgcgcg atctccagcg tggggcctcg
     1981 tcgggtcgcg ggccgtgacc ccaggcgcag ccggctgatc tcgtcgatgg gggtgaccgc
     2041 gcgccagaat tccacgaagc ccggcgactc gacgagcgac cggtaggacg tccgcgccgc
     2101 ccccgacagc gccgccatgg cgcggcgcca ctccatcggc gcaccgggcg gcgcgcccgg
     2161 ctcctccgcg ccggggacgg acggcgcgga ggcctccagc accgcgctca cgatctgctc
     2221 gagatggcga tgcgccagcg cggggtcgcc gtagcgcgcc gcgagcacct cgccctgctc
     2281 ggtgagccgg aagcgcccgc ccacggtgcc cggcggctgg gcccggatgg cgcgccccgc
     2341 ggggccgccc ccgcgggcca cggtcccgcc gcgcccgtgg aagagggtga gcggcacgcc
     2401 ccgtcccgcg cagacgcgca cgatctcttc ctgggcgcga tagagcgccc agttcgccgc
     2461 gaggtagccg ccgtccttgt tgctgtcgga atagccgatc atcaccgtct gctggccccc
     2521 cgcgcgcgcg aggtggtcgc gatagacggg aaagccgaag agatcgtcga ggatgcgcgg
     2581 ggcggcgtcc agatcgccca gggtctcgaa gagcggcacg atggcgaggc ccgcgtcccc
     2641 gcccgcccag cgggcgagga ggagcacggc gaggagatcg gcgggcccgc gggtcatgga
     2701 gatgatgaag gggcccagga gctcgggccc gtagacggcg cgcgcccggg cgagaagccg
     2761 gaacaccgcc caggtctccg ccgcttccgg tgacagcgcc gggctcgcgg gaatctcccc
     2821 cacgggcctg gccaggagcc tcagcagcgt ctccgtgcgc gggcgatcgt cttccccggc
     2881 gaagccggcg ccgagcccca gggcgctcag tagctccccc agcgccgcgg ccagccgcga
     2941 cgattcttcc cggatgtcca gccgcgcggc gtgcagcccg aaggcctcga tctggcgtcg
     3001 cacggtccac aaccggtcat gggccagcgc cggcggcagc gcggaggcga ccaggtccag
     3061 cggccgggtg aggtcggtga ggcggacgcg cgccgcgtgc ggcgtcgcct cgaggaggcg
     3121 cgcggtcatg tcgtcgcgcg ccgccgcctc caggtcggcc gcgagcagcg cgaggatcaa
     3181 ccgatagggc tcgtcggcgt agcgggcctc gagataggcg gcgtgcgcgg gcaggggccg
     3241 gcgggcgacc agccaggcct cgagcgcggc cgggggcggc accc
//
LOCUS       DASUFA010000046         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_233689, whole genome shotgun sequence.
ACCESSION   DASUFA010000046 DASUFA010000000
VERSION     DASUFA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233689"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFX87_01755"
     CDS             complement(<1..812)
                     /locus_tag="VFX87_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX4993656.1"
                     /translation="MYDLLLKGGTVLDPSTKLDGRQDVAVQGGTIARIAPDIAETEAA
                     RTIDVRGRTVVPGLIDLHAHVFEGINRTGVNPDLGGVYSGVTTIVDAGSAGAATFAGF
                     PRHILPNCHTEVIPFLHICQTGLATMPDIIAESSINLDDTLKVVDQHRHLIRGIKARM
                     VSPALEIMGMEMPKLAKRAARESGVKLMVHIGDTEKRYDPKVIRSLLPLLEQGDILTH
                     YFTANPGGVLDGNGKLVPEVKEAADRGVWFDTAHGRMNFSFDVGRRVIEQGVL"
     gene            complement(827..2782)
                     /locus_tag="VFX87_01760"
     CDS             complement(827..2782)
                     /locus_tag="VFX87_01760"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX4993657.1"
                     /translation="MPVTRFEITLRRPLAGEPAYEELKGRLHFAIDPLQAANRRITDV
                     ELAPRDAQGRVTWDSDVSILLPVDRTRCSGRVLLDVVNRGNTVAVPNFNRATRPVFGP
                     GSDANPPVDVGDGFLMNRGWVVISCGWQGDLPRVPGLLGMRTPEARDAQGRPLRGRIY
                     TQVQSTGPVADFLLSDRGHVPYPAADLAEKDALLTVRDQPDGPATEIPRERWRFARME
                     GGRVVPDAGHIHLDGGFEKGRLYQITYTAAGAPVQGLAMAALRDSVAWLKHGGADAGN
                     PAPGRLRWAYGYGRSQTGRLLRTLIYEDLNLDEQGREALDGVIANVAGGMRGEFNQRF
                     GQHSKDRNNMMAHLFPFTDQPQAEPETGAKDALHARLDARGSRLRVCYTNTSAEYHRG
                     DASLIHTDPDGTRDVAPGPYTRVYHFAGTEHGLGIWPPTDSQAAPADPTAPVERSQNL
                     RGVADYSVLLRACLVSLDRWVVDGVEPPPSRHPRLADGTAVPPDRLAAAFARIPGAHY
                     PRHSPRPRRLDWSTLPPAPGKPFGSLVSAVDDDGNEIAGIVLPEIAVPLAAHTGWNLR
                     HGDIGGAEQLLVFAGATIPMARNRTEREATGDPRLSVAERYPSRGAYLDRVRRAAEAL
                     AREGYLLVDDVPLSVAAGARFWDHFTR"
     gene            complement(2805..>3275)
                     /locus_tag="VFX87_01765"
     CDS             complement(2805..>3275)
                     /locus_tag="VFX87_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4993658.1"
                     /translation="SHHDTRPTPQYAQNYDRRSPEGKPEFAVRSQHFTAVDDSLVLCR
                     FTSERGFGLYIEEPYSRMVNAITGWDMTVEELERVGERIINLERLFNVRENVRRKDDA
                     LPWRVMHEPIPEGPSAGAYCPPEELGLMLDRYYALRGWDGDGVPTPARLGALGL"
BASE COUNT          443 a         1149 c         1198 g          485 t
ORIGIN      
        1 agcacgccct gctcgatcac ccggcggccc acgtcgaagc tgaagttcat gcgcccgtgc
       61 gcggtgtcga accacacgcc ccggtcggcc gcctccttca cctccggcac cagcttgccg
      121 ttgccgtcga gcacgccgcc cgggttggcg gtgaagtagt gggtgaggat gtcgccctgc
      181 tcgagcagtg gcagcagcga gcggatcacc ttcgggtcgt agcgcttctc ggtgtccccg
      241 atgtgcacca tgagcttgac gccgctctcc ctggccgccc gcttggcgag cttcggcatc
      301 tccatgccca tgatctcgag cgcgggcgag accatgcgcg ccttgatccc gcggatcagg
      361 tgccggtgct ggtccaccac cttcagcgtg tcgtcgaggt tgatgctgct ctccgcgatg
      421 atgtcgggca tcgtggccag cccggtctgg cagatgtgga gaaacgggat cacctcggta
      481 tggcagttgg gcaggatgtg gcgggggaag cccgcgaagg tggccgcccc cgcgctgccc
      541 gcgtccacga tcgtggtgac gcccgagtag acgccgccca ggtccgggtt caccccggtg
      601 cggttgatgc cctcgaacac gtgggcgtgc aggtcgatca gcccgggcac caccgtccgc
      661 ccgcgcacgt cgatcgtgcg cgcggcctcc gtctccgcga tgtccggcgc gatgcgggcg
      721 atggtacccc cctgcaccgc cacgtcctgt ctgccgtcga gcttggtgga gggatcgagc
      781 acggtgccgc ccttgagcag gaggtcgtac atggggtctc cgatcgtcag cgagtgaagt
      841 gatcccagaa gcgcgcgccc gcggccaccg agagcggcac gtcgtccacc agcaggtagc
      901 cctcgcgggc cagggcctcc gccgcccgcc ggacgcggtc gagatacgcc ccgcgcgacg
      961 gatagcgctc cgcgaccgac aggcgaggat cgccggtggc ctcacgctcc gtgcgattcc
     1021 gcgccattgg tatcgtcgcg cctgcgaaga ccagaagttg ctcggccccg ccgatgtcgc
     1081 catggcgcag gttccatccg gtgtgcgccg ccagtggcac cgcgatctcc ggaaggacga
     1141 tgccggcgat ctcgttgccg tcgtcgtcca ccgcggagac gagggagccg aaaggcttgc
     1201 ccggcgcggg cggtagcgtg ctccagtcga gccggcgcgg ccgcgggctg tggcgcgggt
     1261 agtgagcgcc cgggatccgc gcgaacgccg cggccaggcg atcgggcggc accgcggtgc
     1321 cgtcggccag gcgggggtgg cggctcggcg gcggctcgac gccgtccacc acccagcgat
     1381 cgaggctgac caggcaggcg cgcagcagca ccgagtagtc ggccacgccg cggaggttct
     1441 gggagcgctc gaccggcgcg gtcgggtcgg cgggggccgc ctgcgagtcg gtgggcggcc
     1501 agatgccgag gccgtgctcg gtgcccgcga agtggtacac gcgcgtgtag ggcccgggcg
     1561 ccacgtcgcg cgtgccgtcg ggatcggtgt ggatcaggga ggcgtcgccc cgatggtact
     1621 cggccgacgt gttcgtgtag cacacgcgca gccggctgcc ccgcgcgtcg agccgggcgt
     1681 gcagcgcgtc cttcgccccc gtctccggct ccgcctgcgg ctgatcggtg aacgggaaga
     1741 gatgcgccat catgttgttg cggtccttgg agtgctggcc gaaccgctgg ttgaactcgc
     1801 cgcgcatgcc gcccgccacg ttggcgatga caccgtcgag cgcctcgcgc ccctgctcgt
     1861 cgaggttcag gtcctcgtag atcaaggtgc ggagcagccg cccggtctgc gagcgcccgt
     1921 agccgtaggc ccagcggagc cgccccggcg cgggattgcc cgcgtcggcg ccgccgtgct
     1981 tgagccaggc gacgctgtcg cgcagcgcgg ccatcgcgag cccctgcacc ggcgcgcccg
     2041 cggcggtgta ggtgatctgg tagaggcggc ccttctcgaa gccgccgtcc aggtgaatgt
     2101 ggccggcgtc gggcaccacg cggccgccct ccatgcgggc gaagcgccag cgctcacgcg
     2161 ggatctcggt ggccggcccg tccggctgat cgcgcacggt gaggagcgcg tccttctccg
     2221 cgagatccgc ggccggatag ggcacgtggc cgcggtcgga gagcaggaag tcggcgaccg
     2281 gacccgtgga ctgcacctgg gtgtagatgc ggccgcgcag cggccggccc tgggcgtcgc
     2341 gcgcctccgg ggtgcgcatg ccgaggagcc cgggcacgcg cggcaggtcg ccctgccagc
     2401 cacaggagat caccacccag ccgcgattca tcaagaaccc gtcgcccaca tctacgggcg
     2461 gattcgcatc cgacccgggg ccgaagacgg gccgcgtggc gcggttgaag ttgggcaccg
     2521 ccaccgtgtt gccgcggttg acgacgtcca gcagcacgcg cccgctgcag cgcgtacgat
     2581 ccaccggcag caggatggac acgtccgagt cccacgtcac gcggccctgc gcgtcgcgcg
     2641 gagccagctc cacgtcggtg atgcgccgat tcgccgcctg aagaggatcg atcgcgaaat
     2701 ggagccgccc cttcagctct tcgtaggcgg gctcgcccgc gagcggacgt cgcagcgtga
     2761 tctcgaaccg ggtgacgggc atgggatgac ctcgcgggga cgggctacag gccgagcgcg
     2821 ccgaggcgcg ccggggtcgg caccccgtcg ccgtcccagc cgcgcagggc gtagtagcgg
     2881 tcgagcatca ggcccagctc ctcgggcggg cagtaggcgc ccgcggaggg accctcggga
     2941 atgggctcgt gcatcacgcg ccacggcagc gcgtcgtcct tccggcgcac gttctcccga
     3001 acgttgaaca gtcgctccag gttgatgatc cgctctccca cccgctcgag ctcctccacc
     3061 gtcatgtccc agccggtgat cgcgttgacc atccgggaat acggctcctc gatgtagagg
     3121 ccgaagccgc gctcggaggt gaagcggcag agcacgaggg aatcgtccac cgcggtgaag
     3181 tgctggctgc gcaccgcgaa ctcgggcttg ccctcggggc tgcggcggtc gtagttctgc
     3241 gcgtactgcg gcgtgggccg cgtgtcgtga tggct
//
LOCUS       DASUFA010000047         7303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_812276, whole genome shotgun sequence.
ACCESSION   DASUFA010000047 DASUFA010000000
VERSION     DASUFA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812276"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..645)
                     /locus_tag="VFX87_01770"
     CDS             complement(196..645)
                     /locus_tag="VFX87_01770"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEX4993659.1"
                     /translation="MTAAPDPGAALGPRLGHPFRGPDRLREALTHRSFTNENPGSPHN
                     ERLAFLGDAVLALVVAERLLAAAPGEPVGVLTPRRAALVSGENLARWAAALELGAHLR
                     LGRGEEQMGGRAKESVLATALEAVVGVIYLEAGLDAARAAVARLAVW"
     gene            complement(646..1890)
                     /gene="fabF"
                     /locus_tag="VFX87_01775"
     CDS             complement(646..1890)
                     /gene="fabF"
                     /locus_tag="VFX87_01775"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX4993660.1"
                     /translation="MDARRVVITGAGAVTPIGNTAEEFWTALTEGKSGIGPITRFDAS
                     PLPTRIAGEVKGFDSLRYIDKKDDRKFDPFLKYAVACGVMAVEDAGLNLERVDKNRFG
                     VLVGSGIGGITTLLESHKVLLEKGPDRVSPFFIPMLIVNMASGLLSMRFGAKGPNSSV
                     VTACATGNHAIGDAMRIIQRGDADIMIAGGAEAIIIPLTFAGFCQMKAMSTRNDDPSR
                     ASRPFDATRDGFVCGEGGGLLVLEALEHALARDARIYAEVVGYGMTGDAYHMTAPDPE
                     ADGAARAMALAVRDAGIEPSAVGYINAHGTSTPYNDRSETMAIKRVFGEHARKLAVSS
                     TKSMTGHLLGAAGGIEAIATAFAIHHGILPPTINYETPDPECDLDYIPNHARKQDVEV
                     ALSNAFGFGGTNATIALRKYRP"
     gene            complement(1913..2149)
                     /locus_tag="VFX87_01780"
     CDS             complement(1913..2149)
                     /locus_tag="VFX87_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX4993661.1"
                     /translation="MAKPVEERVKEIIVEQLGVEEDEVLPAAKFIEDLGADSLDTVEL
                     VMAFEEEFDLEIPDEDAEKIATVGDAISYIKENS"
     gene            complement(2175..2927)
                     /gene="fabG"
                     /locus_tag="VFX87_01785"
     CDS             complement(2175..2927)
                     /gene="fabG"
                     /locus_tag="VFX87_01785"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX4993662.1"
                     /translation="MTTGKALAGKVAVVTGGGRGIGLAIARSLAEDGASVVVSGRDAA
                     RLDAAVKELEALGAPALAVPADAGKREDVDRLIEATRERFGRIDVVVNNAGITRDQLL
                     VRMKDDDWDQVIDTNLRGVFLMTRAAAKVMMRQRSGRIINIASAAGAMGNPGQVNYSA
                     AKAGVIGLTKAAARELAHWNILVNAVAPGLIETDMTAAIPAEAREAMLQQVPLKRIGQ
                     SREVGEVVRFLAGDGASYITGQTIHVNGGLYI"
     gene            complement(2924..3874)
                     /gene="fabD"
                     /locus_tag="VFX87_01790"
     CDS             complement(2924..3874)
                     /gene="fabD"
                     /locus_tag="VFX87_01790"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX4993663.1"
                     /translation="MVAGALAFLFPGQGSQEVGMGRALAERSPGAAAVWAEADAALGF
                     PLSTLCFEGPAADLALTANTQPAVLTTSVAAAAALAERGVTPGLAAGHSLGEYSALVV
                     AGALRFADAVRLVRRRGEFMQEAVPVGTGAMAALMGVELAAVEQICAEAAQGEVVGVA
                     NINSPGQIVIAGHRGAVERAVEGAAARGGRKSVMLPVSAPFHCALMKPAADRLVAELE
                     RVAVSDPRIPVVRNVDGGVTRTADEVKPFLVQQVASPVRWTDCMERLAREGATGFLEV
                     GPGRVLTGLLKRTLDGARGHAVEDPASLDRAVAAVGGGAA"
     gene            complement(3868..4848)
                     /locus_tag="VFX87_01795"
     CDS             complement(3868..4848)
                     /locus_tag="VFX87_01795"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX4993664.1"
                     /translation="MNRARIIGVGAFAPKRILTNRELETMVETSDEWIVQRTGIRERH
                     IVADGEGPSDLAVRAAQQAMERAQVTAEEIDFIVVGTTAGDMNFPTTANLVQHRLGCR
                     NAGSVDIYAACSGSVYSLSIGAQYVQTGKYRTVLCIGAECLSRITDFTDRGTCILLAD
                     AAGAAVLRPSTDSAGIIDTDLYSDGRYGDLLIQPAGGSLRPATHETVDQRLHFAKMKG
                     NEVFKVAVRMFGDCAERILKNNGFTASDLDLFVPHQANLRIIEAAVKRLKVPMEKVMV
                     NVERYGNTGAASVYVALEEAWSTGRLKPGDLVLMAAFGGGFTWGAALLRW"
     gene            complement(4845..5870)
                     /gene="plsX"
                     /locus_tag="VFX87_01800"
     CDS             complement(4845..5870)
                     /gene="plsX"
                     /locus_tag="VFX87_01800"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX4993665.1"
                     /translation="MKIAVDAMGGDFGPAVVVEGAVAACREFGLASVLVGDRPAIERE
                     IARLKAQDLAIGVHHASQVVGMAEAPSQALRRKRDSSLRVAAELVKDGECGAIVSAGN
                     TGAAMAISMFVMGVLPGVDRPAIAAPLPSLAGYTVLIDAGANVDPKPRQLFQFAVMGH
                     VYSRHIVGKDNPRVGLLSVGEEEGKGTDLTKEAFEDLRGSSLNFIGNIEGRDIYNGRC
                     DVVVTDGFTGNVCLKVSESLAEMLTAMIREELGRDVLSLAGAALARRAFARLKRRVDY
                     TEMGGAPLLGMNGAAIICHGASPVKAIKNAVRVAADWVRNDVNQHIKTALEAEAVLAE
                     GREGGRE"
     gene            complement(5911..6093)
                     /gene="rpmF"
                     /locus_tag="VFX87_01805"
     CDS             complement(5911..6093)
                     /gene="rpmF"
                     /locus_tag="VFX87_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX4993666.1"
                     /translation="MPLPKRRHSKTRGRKRRTHYKMVAPTRSLCPQCREVKPPHRICP
                     HCGFYKGREVISVEGE"
     gene            complement(6113..6622)
                     /locus_tag="VFX87_01810"
     CDS             complement(6113..6622)
                     /locus_tag="VFX87_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX4993667.1"
                     /translation="MLIQVADIPPEGLRIEGTADFPQPFQDHTWALDELALTVEKDGD
                     TVFVRGDLTARVPLTCGRCVEGYTVTVHPAVDARFVPMPATRGEERELGAGELETDVY
                     QHGTLDLTALLETETTLALPMKPLCREGCRGLCPVCGGNRNATDCACETPAPDSRWAA
                     LKDWAGRTR"
     gene            6786..>7303
                     /locus_tag="VFX87_01815"
     CDS             6786..>7303
                     /locus_tag="VFX87_01815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4993668.1"
                     /translation="MANLMKTGVLLAALTVLVVLIGGALGGQNGMVMAFVLAMVMNLG
                     SYWFSDKIVLAMYRAQPVDEASAPGLYRIVRTLATRAGMPMPRVYIIPGEAPNAFATG
                     RNPQHAAVAVTEGILRIMSEEELEGVLAHELSHVRNRDTLIMAIAATLAGAITYMAHM
                     AQWAMIFGGGRR"
BASE COUNT         1029 a         2668 c         2492 g         1114 t
ORIGIN      
        1 cgcgcgcgcg ggctccacga gaaggtgccc gccccagccg aacgccgccc cccgcacctc
       61 ctgccgctcg acgcggaaga cgtcctagat gttgcggcgc agcagatcct cgacgaccgt
      121 gtagccttcc gcgggcgcgc cgccggcacc gtgcgcgggc gcgagcggaa agggccagag
      181 acgcatgaac gcaggttacc acacggccag acgcgccacg gccgcgcggg ccgcgtcgag
      241 gccggcctcc agatagatca cgcccaccac cgcctcgagc gcggtggcga gcaccgactc
      301 cttcgcgcgc ccgcccatct gctcctcgcc gcgcccgagg cgcagatgcg cgcccagctc
      361 cagcgcggcg gcccagcgcg cgaggttctc gcccgacacc agcgcggcgc gccgtggggt
      421 cagcactccg accggctcgc cgggcgccgc ggcgagcagg cgctcggcga cgacgagggc
      481 cagcaccgcg tcgcccagga aggcgagccg ctcgttatgg ggcgagccgg gattctcgtt
      541 cgtgaaggag cggtgggtga gcgcctcccg cagccggtcg ggaccgcgga aggggtggcc
      601 caggcggggg cccagcgcgg cgcccgggtc cggcgcggcg gtcaactagg gacgatactt
      661 ccggagggcg atcgtggcgt tcgtgccccc gaagccgaac gcgttgctga gcgcaacctc
      721 cacgtcctgc ttgcgggcgt ggttcgggat gtagtccagg tcgcactcgg gatccggcgt
      781 ctcgtagttg atcgtcggcg gcaggatccc gtggtggatc gcgaacgcgg tggcgatcgc
      841 ctcgatcccg cccgccgccc ccagcaggtg gccggtcatg gacttggtgg aggagaccgc
      901 gagcttgcgg gcgtgctcgc cgaagacgcg cttgatcgcc atcgtctccg acctgtcgtt
      961 gtagggcgtg gaggtcccgt gggcgttgat gtagcccacc gccgacggct cgatgcccgc
     1021 gtcgcggacc gccagggcca tggcccgcgc ggcgccgtcc gcttccggat ccggcgcggt
     1081 catgtggtag gcgtcgccgg tcatgccgta gcccaccacc tccgcgtaga tccgcgcgtc
     1141 gcgggccagc gcgtgctcca gcgcctccag caccaggagg ccgccgccct ccccgcacac
     1201 gaagccgtcg cgggtggcgt cgaacggacg gctcgcccgg ctcggatcat cattcctggt
     1261 actcatcgcc ttcatctggc agaagcccgc gaaggtgagc gggatgatga tggcctcggc
     1321 cccgcccgcg atcatgatgt cggcgtcgcc gcgctggatg atccgcatcg cgtcgccgat
     1381 cgcgtgattg ccggtcgcgc acgcggtcac caccgaggag ttcggcccct tggccccgaa
     1441 gcgcatcgag agaaggcccg aggccatgtt cacgatcagc atcgggatga agaagggcga
     1501 cacccggtcc ggccccttct cgagcagcac cttgtggctc tccagcagcg tggtgatccc
     1561 gccgatgccg gagcccacca ggacgccgaa ccgattcttg tccacccgct ccaggttcag
     1621 gcccgcgtcc tccaccgcca tcaccccgca ggccaccgcg tacttgagga acggatcgaa
     1681 cttgcggtcg tccttcttgt cgatgtagcg gagcgagtcg aagcccttca cctcacccgc
     1741 gatccgggtg ggcaggggac tggcgtcgaa ccgggtgatg ggcccgatcc cggacttccc
     1801 ctccgtcagg gcggtccaga actcctcggc ggtgttgccg atcggcgtga ccgcccccgc
     1861 cccggtgatg acgactcgac gcgcgtccac gcgcccctcc gtgcccgcgc ccctacgagt
     1921 tctctttgat gtagctgatg gcgtccccga ccgtggcgat cttctcggca tcctcgtccg
     1981 ggatctcgag atcgaactcc tcctcgaagg ccatcacgag ctcgacggtg tcgagagagt
     2041 cggctccgag gtcctcgatg aacttcgcgg ccggcaacac ctcgtcctct tccactccga
     2101 gctgctcgac gatgatctcc ttgacccgct cctcgaccgg cttcgccatg ccctcccctc
     2161 ctggctgcga ttgattagat gtacagtccg ccgttgacgt ggatcgtctg gccggtgatg
     2221 tagctcgcgc cgtcgccggc gaggaaccgc accacctccc ccacctcacg gctctggccg
     2281 atccgcttga gcggcacctg ctggagcatc gcctcgcggg cctccgcggg gatcgcggcc
     2341 gtcatgtccg tctcgatgag ccccggcgcc accgcgttca ccaggatgtt ccagtgcgcc
     2401 agctcccgcg ccgccgcctt cgtgagcccg atcacgcccg ccttggccgc cgagtagttc
     2461 acctgcccgg ggttgcccat cgcccccgcg gcggacgcga tgttgatgat acgcccgctc
     2521 ctctgcctca tcatcacctt ggccgccgcg cgggtcatca ggaatacccc gcgcaggttc
     2581 gtgtcgatca cctggtccca gtcgtcgtcc ttcatccgga ccaggagctg atcgcgcgtg
     2641 atcccggcgt tgttgacgac cacgtcgatg cgcccgaagc gctcccgggt cgcctcgatc
     2701 aggcggtcga catcttcccg cttcccggca tccgccggaa ccgccagggc cggcgcgccc
     2761 agggcctcca gctccttcac cgccgcgtcg agacgcgccg cgtcgcggcc agagactacc
     2821 acggaggcgc cgtcctctgc cagcgaccgg gcgatggcca gcccgatgcc ccggccgccg
     2881 ccggtcacga ccgcaacctt tcccgccagg gctttgcccg tggtcacgcg gctccgcctc
     2941 cgaccgccgc gaccgctcgg tcgagcgacg ccggatcctc caccgcgtgc ccccgcgccc
     3001 cgtcgagcgt gcgcttgagc agcccggtca ggacgcggcc gggcccgacc tcgagaaatc
     3061 cggtcgcgcc ctcgcgcgcg agccgctcca tgcagtcggt ccagcgcacc gggctcgcca
     3121 cctgctggac caggaacggc ttcacctcgt cggcggtccg ggtcaccccg ccgtccacgt
     3181 tgcggactac cggaatacgc gggtcgctca cggcgacccg ctctagctct gcgaccaggc
     3241 ggtcggcggc gggcttcatc agcgcgcagt ggaagggcgc gctcaccggc agcatcacgc
     3301 tcttgcggcc gccgcgcgcc gccgctccct cgaccgcgcg ctccaccgcc ccccggtgcc
     3361 ccgcgatgac gatctggccc ggggagttga tgttggccac ccccaccacc tcgccctgcg
     3421 cagcctccgc gcagatctgc tcgacagcgg ccagctccac gcccatcagc gcggccatcg
     3481 cgcccgtgcc caccggcacc gcctcctgca tgaactcgcc gcggcggcgc accagccgca
     3541 ccgcgtccgc gaagcggagg gcccccgcga ccacgagggc cgagtactcg cccaggctgt
     3601 ggccggccgc gagaccgggg gtcacgcccc gctcggccag cgccgccgcg gcggccacgc
     3661 tcgtggtcag caccgcgggc tgggtgttgg cggtcagggc caggtcggcg gccgggcctt
     3721 cgaagcagag cgtcgagagc gggaagccga gcgcggcatc cgcctcggcc cagaccgcgg
     3781 cggcgccggg cgagcgctcc gcgagcgcgc ggcccatccc gacctcctgc gagccctggc
     3841 cggggaagag gaaggcgagc gcgccggcta ccatcgcagc agcgccgcgc cccaggtgaa
     3901 gccgccgccg aaggcggcca tcagcaccag atcgccgggc ttgagccgcc cggtggacca
     3961 cgcctcctcg agtgccacgt agaccgaggc ggcgccggtg ttgccgtagc gctccacgtt
     4021 gaccatgact ttctccatcg gcaccttgag gcgcttgacc gccgcctcga tgatgcggag
     4081 gttcgcctgg tggggcacga agaggtcgag gtcgctcgcg gtgaagccgt tgttcttgag
     4141 gatgcgctcg gcgcagtcgc cgaacatccg caccgccacc ttgaagacct cgttgccctt
     4201 catcttggcg aagtgcaggc gctggtccac cgtctcgtgg gtggcgggcc gcagggagcc
     4261 cccggcgggc tggatcagca gatcgccgta gcggccgtcc gagtagagat cggtgtcgat
     4321 gatgccggcg gagtcggtgg aaggccggag caccgcggcg cccgcggcgt cggcgagcag
     4381 gatgcaggtg ccgcggtcgg tgaagtcggt gatgcgcgag aggcactcgg cgccgatgca
     4441 cagcacggtg cggtacttgc cggtctggac gtactgggcg ccgatggaca ggctgtagac
     4501 cgagcccgag caggccgcgt agatgtccac cgagcccgcg ttgcggcagc ccagacgatg
     4561 ctggacgaga ttggcggtgg tggggaagtt catgtccccc gcggtggtgc cgaccacgat
     4621 gaagtcgatc tcctccgcgg tgacctgggc ccgctccatc gcctgctggg cggcccggac
     4681 cgccaggtcc gaggggccct cgccgtcggc cacgatgtgg cgctcccgga tgccggtgcg
     4741 ctggacgatc cactcgtcgg aagtctcgac catcgtctcg agctcccggt tcgtcaggat
     4801 ccgcttcggt gcgaacgcgc ccacgccgat gatgcgagcc cggttcattc ccggcctccc
     4861 tcccgtccct cggcgaggac agcttccgcc tcgagcgcgg tcttgatgtg ctggttcacg
     4921 tcgttgcgaa cccagtcggc ggccacgcgt accgcgttct tgatcgcctt caccggagaa
     4981 gccccgtggc agatgatggc ggccccgttc atgccgagca acggcgcgcc gcccatctcg
     5041 gtgtagtcca cccggcgctt gaggcgcgcg aaggcccgcc gggcgagcgc ggcgcccgcc
     5101 agcgagagca cgtcgcgccc cagctcctcg cgaatcatcg cggtgagcat ctcggccagg
     5161 ctctcggaga ccttcaggca gacattgccg gtgaagccgt ccgtcaccac cacgtcgcag
     5221 cggccgttgt agatgtcgcg cccctcgatg ttgccgatga agttgagcga cgagccgcgc
     5281 agatcctcga acgcttcctt ggtcaggtcg gtccccttgc cctcttcctc gcccaccgag
     5341 agcagcccga cccgcggatt gtccttgccc acgatgtgcc gcgagtagac gtggcccatg
     5401 accgcgaact ggaagagctg gcgcggcttg gggtccacgt tggcgcccgc gtcgatgagc
     5461 acggtgtaac cggccaggct cggcagcggc gccgcgatgg cgggccggtc caccccgggc
     5521 agcacgccca tcacgaacat gctgatcgcc atggccgccc cggtattgcc ggcggagacg
     5581 atggccccgc actcgccgtc cttcacgagc tcggcggcca cgcggagcga ggagtcgcgc
     5641 ttgcggcgga gcgcctgcga gggcgcctcg gccatgccga ccacctggga ggcgtggtgc
     5701 acgccgatcg cgagatcctg ggccttgagc cgcgcgatct cgcgctcgat ggccggccgg
     5761 tctcccacga gcaccgaggc cagcccgaac tcgcggcacg cggccaccgc gccctcgacg
     5821 acgacggcgg ggccgaagtc gcctcccatg gcgtcgaccg cgatcttcat ggcgccgatc
     5881 ttatggcacg gccccgctca gccggaaaaa ctattctcct tcgaccgaga tgacttcccg
     5941 tcccttgtag aagccgcagt gggggcagat gcggtggggc ggcttgacct cgcggcactg
     6001 ggggcagaga gagcgcgtgg gcgcgaccat cttgtagtgc gtgcgccgct tgcggccgcg
     6061 cgtcttggag tgtcgtcgct tgggcagagg catcggaggg ctcctgttcg ggctatctcg
     6121 tccggccggc ccagtccttc agggccgccc agcgggagtc gggcgcgggc gtctcgcacg
     6181 cgcagtcggt ggcgttgcgg ttgccgccgc agacggggca gaggccccgg cagccttcgc
     6241 ggcagagcgg cttcatcggc aaggccaggg tggtctcggt ctcgagcagg gccgtgagat
     6301 cgagggtgcc gtgctggtag acgtcggtct cgagctcgcc cgcgcccagc tcgcgctcct
     6361 cgccgcgcgt cgccggcatg ggcacgaaac gggcatccac cgccgggtgg accgtcaccg
     6421 tgtagccctc gacgcagcgc ccgcaggtga gcggcacgcg cgcggtgaga tcgccgcgga
     6481 cgaagaccgt gtcgccgtcc ttctcgacgg tcagcgcgag ctcgtcgagc gcccaggtgt
     6541 ggtcctggaa cggctgcggg aagtccgcgg tcccctcgat gcggagaccc tcgggtggaa
     6601 tgtcggctac ctggatcagc acgtgaagaa cctcgtctaa gcttctgtca agtaaggagt
     6661 ttttcttaca gacgcgtcag tataagcgtg ggggtacccc ttgtcaaccc aaaagcccgt
     6721 aaagtcgcca cttgcttgac ggtctggggc tcggctgcta gcattcggac cgggaggacg
     6781 agcgcatggc gaacctcatg aaaaccggtg tcctgctggc ggccctcacg gtgctggtcg
     6841 tgctcatcgg cggggcgctc ggtggtcaaa atggcatggt catggccttc gtgctggcca
     6901 tggtgatgaa cctcggcagc tactggttct cggacaagat cgtcctggcg atgtaccggg
     6961 cccagccggt ggacgaggcc tccgcgcccg ggctctaccg gatcgtgcgg accctggcca
     7021 cccgcgccgg catgccgatg ccgcgggtct acatcatccc gggcgaggcc ccgaacgcct
     7081 tcgcgacggg ccgcaacccg cagcacgcgg cggtcgccgt cacggaaggc atcttgcgca
     7141 tcatgagtga ggaagagctg gaaggggtgc tggcccacga gctctcccac gtgcggaacc
     7201 gcgacacgct gatcatggcc atcgcggcca ccctggccgg ggcgatcacc tacatggccc
     7261 acatggccca gtgggcgatg atcttcggcg gcggccggcg cga
//
LOCUS       DASUFA010000048         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_697782, whole genome shotgun sequence.
ACCESSION   DASUFA010000048 DASUFA010000000
VERSION     DASUFA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697782"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VFX87_01820"
     CDS             complement(<1..116)
                     /locus_tag="VFX87_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4993669.1"
                     /translation="MPGALAGIRVVDLTNNQAGPSCGQILAWLGADVVKVEEP"
     gene            complement(146..1393)
                     /locus_tag="VFX87_01825"
     CDS             complement(146..1393)
                     /locus_tag="VFX87_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX4993670.1"
                     /translation="MTTARVSACIVGGGPAGLMLGLLLAKRGADVLVLEGHEDFEREF
                     RGEVLQPSTAHLLDELGLLDYIRAQPHSLLEAGKVRLDGKEVGEFAFKKIAPRYPYAI
                     WMPQPIFLDALLRKATPFPSFQCWMGAKVTKLVEEDGRVVGVSGLRHGQEPFEVRADV
                     VVGADGRHSVVATLGGFTPEYEHHDFDVIWFTIDQPPGWSSTFYVSLGEVRGLMLPKY
                     PHHIQAGIILPTGEWRQWRKEGVAAVAARVRRFDSIFNGFADSLKDFTPFFPLDGTIR
                     FMREWARDGLLLIGDAAHTMSPAGAIGVNVAIATAAVAAQEIFPRLGKGPIPRADLLA
                     VQRLREDDVRTLHRLQLGAQRVLLNQGSRNPLVKWLVSTALPIFLRSPLLPRVQRRIF
                     FGAPLPPLDPAFSFRETRDTVST"
     gene            1469..1945
                     /locus_tag="VFX87_01830"
     CDS             1469..1945
                     /locus_tag="VFX87_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4993671.1"
                     /translation="MATLQLRVNGSARTIQLDDPDTPLLYVLRNDLDLTGAHFGCGLA
                     QCGACTVHVDGAAVRSCVVPAASVQGRAVTTIEGLGSPARPDPLQAAFIAEQAAQCGF
                     CTAGIVMSARALLDRNPKASETEVRQALAGNLCRCGSHARVLRAVMRVAAGRTGSA"
     gene            1947..>2203
                     /locus_tag="VFX87_01835"
     CDS             1947..>2203
                     /locus_tag="VFX87_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4993672.1"
                     /translation="MAAPEISRRDVLAAGLKAGGVLVVGLTFGGRPLPAVAQTIANAD
                     RFLGKPLAPDQVDSFLAIHRDGTLTLFTGKVDIGTGGRIALR"
BASE COUNT          327 a          801 c          767 g          308 t
ORIGIN      
        1 ggctcctcca ccttgaccac gtcggcaccg agccaggcca ggatctggcc gcacgagggg
       61 cccgcttggt tattggtgag gtcgacgaca cggatccccg cgagtgcgcc cggcatggcc
      121 acacatcttt actacagcgc cggcttcagg tcgagaccgt gtcgcgcgtc tcccggaacg
      181 agaacgccgg gtccagcggg ggcaggggcg cgccgaagaa gatgcgccgc tgcacccggg
      241 gcaggagcgg cgagcgcagg aagatcggca gcgccgtgga taccagccat ttcacgaggg
      301 ggttgcgact gccctggttc agcagtacgc gctgggcacc gagctgcagg cgatgcaagg
      361 tgcggacatc gtcctcccgc agccgctgca cggccagaag gtccgcgcgc gggatggggc
      421 ccttgcccag gcgcggaaag atctcctggg ccgccaccgc cgcggtggcg atggccacgt
      481 tgaccccgat ggcgccggcc gggctcatgg tatgggccgc gtcgccgatg aggagcaggc
      541 cgtcgcgcgc ccactcgcgc atgaagcgga tggtgccgtc cagcgggaag aagggcgtga
      601 agtccttcag cgagtcggcg aagccgttga agatggagtc gaagcggcgc acgcgcgcgg
      661 ccaccgccgc cacgccctcc ttgcgccact gccgccactc gccggtgggc aggatgatgc
      721 cggcctggat gtgatgcggg tacttgggca gcatgaggcc gcggacctcg cccagcgaca
      781 cgtagaaggt gctggaccag ccgggcggct ggtcgatggt gaaccagatc acgtcgaagt
      841 cgtgatgctc gtattccggc gtgaacccgc ccagcgtggc caccaccgag tggcggccgt
      901 cggcgcccac caccacgtcc gcccggacct cgaagggttc ctgcccgtgg cggagcccgc
      961 tcacgcccac cacccgcccg tcctcctcga cgagcttggt caccttggcg cccatccagc
     1021 actggaagga cgggaagggc gtggccttgc gaagcagcgc gtcgaggaag atcggctgcg
     1081 gcatccagat ggcgtaggga taccggggcg cgatcttctt gaaggcgaat tcgccgacct
     1141 ccttgccgtc gagccggacc ttgcccgcct ccagcagcga gtggggctgg gcccggatgt
     1201 agtcgagcag ccccagctcg tccaggaggt gggcggtgct gggctggagc acctcgccgc
     1261 ggaactcgcg ctcgaaatcc tcgtgccctt ccagcaccag gacgtcggcg ccccgcttgg
     1321 ccaggagcag gcccagcatc agccccgccg ggcccccgcc cacgatgcac gcgctcacgc
     1381 gcgcggtggt catggcccga ggatatcgca tcagtcggaa ggccaggaga ccttgactcc
     1441 gtcgcgcgcc catcgcaacc tcagggccat ggccaccctc cagctccgcg tgaacggcag
     1501 cgcccgcacc atccagctcg acgatcccga cacgccgctc ctctacgtcc tgcgaaacga
     1561 tctcgatctc accggcgccc acttcggctg cgggctggcc cagtgcggcg cgtgcaccgt
     1621 gcacgtggac ggcgccgcgg tgcggtcctg cgtcgtcccc gcggcgagcg tccagggccg
     1681 cgcggtgacc accatcgagg ggctcggctc gcccgcgcgg ccggacccgc tccaggcggc
     1741 cttcatcgcc gagcaggcgg cccagtgcgg cttctgcacc gccggcatcg tcatgagcgc
     1801 gcgcgccttg cttgatcgca acccgaaggc cagcgagacc gaggtgcgtc aggcgctggc
     1861 cggcaatctc tgccgctgcg gcagccatgc ccgcgtgctg cgcgcggtca tgcgcgtggc
     1921 cgccggccgg acggggagcg cctgagatgg ccgcgcccga gatctcccgc cgtgacgtcc
     1981 tcgcggcggg gctcaaggcc ggcggcgtgc tcgtggtcgg gctcaccttc ggcggccggc
     2041 cgctgcccgc cgtcgcccag accattgcca acgccgatcg cttcctcggc aagccgctgg
     2101 cccccgatca ggtggactct ttccttgcca tccaccgcga cggcacgctg acgctcttca
     2161 ccggcaaggt ggacatcggc accggtggcc gcatcgcgct gcg
//
LOCUS       DASUFA010000049         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1499386, whole genome shotgun sequence.
ACCESSION   DASUFA010000049 DASUFA010000000
VERSION     DASUFA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499386"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFX87_01840"
     CDS             complement(<1..350)
                     /locus_tag="VFX87_01840"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEX4993673.1"
                     /translation="MRYISNTPAQQKDMLGRIGAASIEALLANVPAKARLSRPLGLSA
                     AMAETDLVRHLRALAARDASADDYACFLGAGAYDHIVPSPINHLISRGEFFTAYTPYQ
                     PEASQGTLRTIFEY"
     gene            complement(363..755)
                     /gene="gcvH"
                     /locus_tag="VFX87_01845"
     CDS             complement(363..755)
                     /gene="gcvH"
                     /locus_tag="VFX87_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX4993674.1"
                     /translation="MSNVPADLKYTKEHEWAKIEGDRARIGITAFAQEQLGDVVFVEL
                     PKVGAKVTSMATFGVVESVKAVSDLFAPLTGEVVEINAELPKKPETVNRDPYGQGWMI
                     VIRMTSPAEASALLSAAQYEQLIAAGGH"
     gene            complement(765..1880)
                     /gene="gcvT"
                     /locus_tag="VFX87_01850"
     CDS             complement(765..1880)
                     /gene="gcvT"
                     /locus_tag="VFX87_01850"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX4993675.1"
                     /translation="MTAHESGPLKRTPLHDVHVKAGAKMVPFGGWDMPVQYAGIVEEH
                     KAVRSAVGLFDISHMGEFEVAGGSALAGVQRLCTNDAAPLAEGQVQYSLLCYPEGGIV
                     DDLTLYRLAGDRYMLTVNASNIDKDWRWVTEHEPGAAWKNVSAETALLAVQGPKAEGL
                     VQRLADTDVVSIRYYHFGRGKVGGVPAVISRTGYTGEDGFELYVPAGEAARLWHALME
                     AGRPDGIQPIGLGARDTLRLEMKFALYGNDIDQTTNPLEAGLGWVVKPAKGEFIGRAA
                     IEALRARGVARKLVGFEMVERQVPRHGYRILEGGAEIGVVTSGSFSPILERGIGIGYV
                     RADLAAVGSEWEVEIRGAGQRARVVRTPFVPSKAKKS"
     gene            complement(1933..2268)
                     /locus_tag="VFX87_01855"
     CDS             complement(1933..2268)
                     /locus_tag="VFX87_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993676.1"
                     /translation="MEADDVVHRTGEGQRWKATAIAGVEYCGLRSHGADGGGSFLVRV
                     ARGARVPRHDHPGGEEIYVVGGRARIGGRPVKTGDYFWTPPGGAHDLVADEETLIFVN
                     APRGVVFLE"
     gene            complement(2287..>3095)
                     /locus_tag="VFX87_01860"
     CDS             complement(2287..>3095)
                     /locus_tag="VFX87_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4993677.1"
                     /translation="GDVLLQSVVNEEHAGNGTLDLVARGQRADAALVLEPTECRLFVT
                     APGGLYWELEVPGVPTSPGARWQGTRQVGVSAVEKLPCAIEALLAVEREYNARPAHPL
                     HRDGNPFALVLGKVTGGHYETVTALSAILRGSAYFAPGSGEIADVMAAMRAAVARLNA
                     ADPFLAAHPARLRFLHHDDGVAQPAALPFAAEVASALGAAGIAAAPSGGPFCCDIRHL
                     VNQGGMPAAIFGPGSIGRAHRPDEFVPLAEYHAAIEGLIELLVRWCGILE"
BASE COUNT          447 a         1124 c         1061 g          463 t
ORIGIN      
        1 tggtactcga agatggtgcg gagcgtgccc tggctcgcct cgggctgata gggcgtatag
       61 gcggtgaaga attctccgcg ggagatgagg tggttgatcg ggctcggcac gatgtgatcg
      121 taggcgccgg cgccgaggaa gcaggcgtag tcgtcggcgg aagcgtcccg cgcggcgagc
      181 gcgcggaggt ggcggactag gtccgtctcc gccatcgcgg cggacaagcc gagcggccgc
      241 gagagccgcg ccttggccgg cacattggcg agcaacgcct cgatgctcgc cgcgccgatc
      301 cggcccagca tgtccttctg ctgggcggga gtattcgaga tatagcgcat gaccgatatc
      361 cctcagtgcc cgccggcggc gatgagctgc tcgtactggg ccgcgctcag gagcgcgctc
      421 gcctcggcgg gactggtcat gcggatcacg atcatccagc cctggccata ggggtcgcgg
      481 ttcaccgtct ccggcttctt gggcagctcg gcattgatct ccaccacctc gccggtgagg
      541 ggggcgaaga gatccgacac cgccttcacc gactccacca cgccgaaggt agccatgctg
      601 gtgaccttgg cgcccacctt gggcagctcg acgaacacca cgtcgccgag ctgctcctgg
      661 gcgaaggcgg tgatgccgat gcgggcgcga tcgccctcga tcttcgccca ctcgtgctct
      721 ttcgtgtact tcaggtccgc cggcacattg gacatcacgc ccccctacga cttcttggcc
      781 ttggaaggaa cgaacggtgt gcggacgacg cgggcccgct ggcccgcgcc acggatctcg
      841 acctcccact cgctgcccac ggcggcgaga tccgcgcgca cgtacccgat cccgatgccg
      901 cgctccagga tgggcgagaa cgagcccgac gtcaccactc cgatctccgc gccgccctcg
      961 aggattcgat aaccgtggcg cggcacctgg cgctcgacca tctcgaagcc gacgagcttg
     1021 cgcgccaccc cacgggcgcg cagggcttcg atggccgccc gcccgatgaa ctcgcccttg
     1081 gcgggcttga ccacccagcc gaggccggct tccagcggat tggtggtctg gtcgatgtcg
     1141 ttgccgtaca gcgcgaattt catctcgagc cgcagcgtgt cgcgggcgcc gagcccgatg
     1201 ggctggatgc cgtcgggccg gcccgcctcc atgagggcgt gccagagccg cgccgcctcc
     1261 cccgcgggga catagagctc gaagccgtcc tcgccggtat agccggtgcg ggagatgacg
     1321 gcgggcactc cccccacctt gccccggccg aagtggtagt agcggatcga gaccacgtcg
     1381 gtgtcggcga gccgctgcac cagcccttcc gccttcgggc cctgcacggc caggagggcc
     1441 gtctccgcgc tcacgttctt ccaggccgcg ccgggctcgt gctcggtcac ccagcgccag
     1501 tccttgtcga tattggacgc gttcacggtg agcatgtagc ggtcgccggc caggcggtag
     1561 agggtgaggt cgtccacgat gccgccctcg ggatagcaga gcagcgagta ctgcacctgg
     1621 ccctccgcca gcggggcggc gtcgttggtg caaagccgtt gcaccccggc gagcgcggag
     1681 ccgccggcca cctcgaactc ccccatgtgg ctgatgtcga agaggcccac cgcggagcgc
     1741 accgccttgt gctcctcgac gatgcccgcg tactgcacgg gcatgtccca gccgccgaag
     1801 ggaaccatct tcgctccggc cttgacgtgg acgtcatgga gcggcgtgcg cttgagtggg
     1861 ccggattcgt gtgcggtcat cggacagccg gcgtcatctt aaccgaagga aaccgagccc
     1921 gcaattgcgg cttcactcga ggaacacgac cccgcggggc gcattcacga agatgagggt
     1981 ctcctcgtcc gccaccaggt cgtgcgcgcc gccgggcggc gtccagaagt agtcgcccgt
     2041 cttcacgggc cggccgccga tgcgcgcgcg cccgcccacc acgtagatct cctcgccgcc
     2101 cgggtggtcg tgccgcggga cccgcgcgcc ccgggcgacc cgcaccagga acgacccgcc
     2161 cccgtcggct ccgtggctcc gcagcccgca gtattccact cccgcgatgg cggtggcttt
     2221 ccagcgctgc ccttccccgg tccggtgcac cacgtcgtcg gcttccatgc ccgtcctcct
     2281 cggccgctat tcgagtatgc cgcaccagcg cacgagcagc tcgatcaggc cctcgatggc
     2341 ggcatggtac tcggccagcg gcacgaactc gtcgggacga tgcgcccggc cgatgctgcc
     2401 cggtccgaat atcgccgccg gcatgccgcc ctggttcacg aggtgtcgga tgtcgcagca
     2461 gaagggcccg ccgctcggcg cggcggcgat cccggcggcg cccagcgccg acgccacctc
     2521 ggcggcgaag ggcagcgcgg cgggctgggc cacgccgtcg tcgtggtgga gaaagcgcag
     2581 ccgggccggg tgcgcggcga ggaagggatc ggcggcgttc agccgcgcca ccgccgcgcg
     2641 catggccgcc atcacgtccg cgatttcccc cgatcccggc gcgaagtacg ccgagccgcg
     2701 caggatggcg gacagtgcgg tcaccgtctc gtagtggccg cccgtcacct tgcccagcac
     2761 gagggcgaag gggttgccat cgcggtgcag cggatgggcg ggccgcgcat tgtactcgcg
     2821 ctccaccgcg agcagcgcct cgatcgcgca gggaagcttc tccaccgcgc tcacgcccac
     2881 ctggcgggtc ccctgccagc gggccccggg cgaggtcggc acccccggca cctccagctc
     2941 ccagtacagg ccccccggcg cggtgacgaa gaggcggcac tcggtgggct cgagaaccag
     3001 cgcagcgtcg gcgcgctgac cacgggcaac gaggtccaag gtcccgttgc cggcatgctc
     3061 ctcgttgacc acgctctgga ggagcacgtc gccgc
//
LOCUS       DASUFA010000050         7001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_812575, whole genome shotgun sequence.
ACCESSION   DASUFA010000050 DASUFA010000000
VERSION     DASUFA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812575"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFX87_01865"
     CDS             <1..243
                     /locus_tag="VFX87_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX4993678.1"
                     /translation="AWHTALDLGSLLTVAECCAMAALERKESRGGHTREDHPYTDDQW
                     GTVNLVSRLRDGALELRREPLPQMPAELRTLFQERK"
     gene            243..998
                     /locus_tag="VFX87_01870"
     CDS             243..998
                     /locus_tag="VFX87_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX4993679.1"
                     /translation="MATTFTMRVFRGSPEGGDFKEYRVEAGEGMVVLDLIHRIQATQA
                     NDLACRWNCKAGKCGSCSAEINGKPRLMCMTRMDTFKEGEPISVAPIRTFPLIRDLVT
                     DVSYNYEKAKQVPPLRLKPPDPDGKYRMMQEDVDRGQEFHKCIECFLCQNVCHVIRDH
                     DLKKRFAGPRFFIKIAGLEMHPLDTRDRTEFVREQAGVGLCNITKCCTEVCPEHIHIT
                     DNGIIPLKERAADNLDPIVWIMRKIRGHKKAAP"
     gene            1003..2193
                     /gene="sucC"
                     /locus_tag="VFX87_01875"
     CDS             1003..2193
                     /gene="sucC"
                     /locus_tag="VFX87_01875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX4993680.1"
                     /translation="MKIHEYQGKAILKEFGVPVPGGDVADTPAQARRIAEGLGGRVVV
                     KAQVHAGGRGKAGGVKLADDPAAAEAAAKQILGMMLKTPQTPPEGIRVLKVLVEEARP
                     IATELYLSVTLDRGRGTHVVMASQAGGMEIEEVAAKHPEKIIREWTDPALGLQAFQAR
                     NLAFGLGLAGDQFKAGVALIVNLFRAYLDKDCSLAEINPLVITTDGRVLALDAKLNFD
                     DNALYRHKDVVALRDINEETPLDVEASKYGLNYIKLDGSVGCMVNGAGLAMATMDIIK
                     LAGGEPANFLDVGGGASPEQIENAFRILSSDPSVKAVFINVFGGILRVDRLAEGIIAA
                     VKKLGLELPVVLRAEGTNVEQGKKMLAESGLALVMASDMGDGARKVVELARGPRKIAL
                     RPDK"
     gene            2190..3068
                     /gene="sucD"
                     /locus_tag="VFX87_01880"
     CDS             2190..3068
                     /gene="sucD"
                     /locus_tag="VFX87_01880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX4993681.1"
                     /translation="MSVLVDKNTRVLVQGITGKEGAFHAGRCKEYGTQVVGGVTPGKG
                     GTTHEGFPVWNTVEDAVKATGADCALIFVPPPAAADAIMEAADAGMPLVVCITEGVPV
                     ADMVRVKAFLSRTTSRLIGPNCPGLITPGQAKIGIMPGHIHKVGNVGVVSRSGTLTYE
                     VVHQLTQRGLGQSSCVGIGGDPVNGTNFIDVLELFQADPETTSIMMIGEIGGTAEEEA
                     AAYIKAHVTKPVVGFICGQTAPPGRRMGHAGAIIAGGKGTAAEKMAAMEAAGITTVKS
                     PADMGATVARVVGKKG"
     gene            3082..3507
                     /gene="ndk"
                     /locus_tag="VFX87_01885"
     CDS             3082..3507
                     /gene="ndk"
                     /locus_tag="VFX87_01885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX4993682.1"
                     /translation="MAVQRTLTIIKPDAVAKGVSGQIIARFEQEGLKVLAAKLIHLSA
                     AQAGGFYIVHKERPFYGSLCAFMTQGPCMPIVLEGENAITRLRDLMGATDPAKAAPGT
                     IRKDFASSIEANAVHGSDSPESAAFEIPYFFGALEIFPR"
     gene            3521..4045
                     /locus_tag="VFX87_01890"
     CDS             3521..4045
                     /locus_tag="VFX87_01890"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX4993683.1"
                     /translation="MHFPAVLGRKQRLLAGVVGIGLGFGGPFLLSVALVASSGDASLL
                     ILPLPFLGALWVIQGLAPSGYTLEEGGLRLERRWARRLIPYDRITGCDRERRPIGGLL
                     AAGVNSLFGSHGPRWNPRTGWHYLAISNTDDLVFVHTRGGLVVLSPSRPDEFVAALYA
                     ELAAKPASWPTRSS"
     gene            4024..4455
                     /gene="arfB"
                     /locus_tag="VFX87_01895"
     CDS             4024..4455
                     /gene="arfB"
                     /locus_tag="VFX87_01895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX4993684.1"
                     /translation="MADPIVVTADVRVPASALTVHAVRASGPGGQNVNKVATKIDLRV
                     DLAALEGLPDDARPRLQRLAQNRLDADGRLVVISQVTRNQARNLEDARARVADLIRAA
                     LVAPRRRVKTRPSAGAKHRRLAGKKRRGDVKRWRARPGEAD"
     gene            complement(4468..4854)
                     /locus_tag="VFX87_01900"
     CDS             complement(4468..4854)
                     /locus_tag="VFX87_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX4993685.1"
                     /translation="MPSVEHLVARTLLAGGLLGIALILAGGALYAVHGGFHHHTLYLN
                     RPPGGSPPGVFTSVRQVLDGLRRRSLDPLAVTALGLVGLMATPIVAVALAIPAFLRAG
                     DRRYAAIAAIVLAMLVVSLTVAGGIH"
     gene            complement(4893..5741)
                     /locus_tag="VFX87_01905"
     CDS             complement(4893..5741)
                     /locus_tag="VFX87_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4993686.1"
                     /translation="MSLVVFSIAVLFTSIGAGVVGAILGLGGGILLIPILTLFYGVDI
                     HYAMGASIISVIATSSGAAAAYLRTGLSNLRVGLFLVMATISGAMLGAFLAGVVPVRA
                     LELILGLALSYSAFTIIRQLQDELPERLPEDPLAIRFGLEGRYYDFHLQREVDYRARR
                     VGFGLTAMFGAGLLSGMLGIGSGAFKVLAMDYLMRLPMKVSTATSNFMIGLTASASAG
                     IYFSRGDINALIVAPVAMGVLAGAYLGSILMARMRNTTVRKLFLPIVVYLAISMILRG
                     LGIHLL"
     gene            5945..>7001
                     /locus_tag="VFX87_01910"
     CDS             5945..>7001
                     /locus_tag="VFX87_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4993687.1"
                     /translation="MAAAIDVPDRFNAATYFLDRHVTEGRGARTAFRFAGRDVAYGEI
                     ALRANRLGNALRARDVQIEQRVLLALPDRPEFAEAFWGAMKIGAVPVPVSDALPAEEL
                     AFLLQDSRARAVIASESAATEILKIRAQCPALESVIVVGGRRRGALEYERLLEKASPE
                     LTAADTSRDDVALWLYTSGSTGRPKAAVHLHHHLVYAAELVGRGTFGIAPDDVIFSAS
                     KLHFAFGLGNSLYFPALVGAASVLVPERLEAEQVFALIQAERPTMLFAVATLYRRLLQ
                     VEGAERRFDLSSLRLCVSSGEALPAGIFHAWKSRFGHELLDVVGSTEALHDYIANRPG
                     EARPGSSGRVIPGYEARL"
BASE COUNT         1141 a         2407 c         2485 g          968 t
ORIGIN      
        1 gcctggcaca ccgcgctcga cctgggctcg ctgctcacgg tggccgagtg ctgcgcgatg
       61 gcggcgctgg agcggaagga gagccgcggc ggccacactc gcgaggatca tccttacacc
      121 gacgatcaat gggggacggt caacctggtg tcacggctgc gcgacggcgc gctcgagctg
      181 cgacgcgagc cgcttcccca gatgcccgcc gagctccgga ccctcttcca ggagagaaag
      241 tgatggcgac gaccttcacg atgcgggtgt tccgaggcag ccccgagggt ggcgacttca
      301 aggagtaccg ggtcgaggcc ggcgagggca tggtcgtgct cgatctcatc caccgcatcc
      361 aggccaccca ggccaacgac ctggcctgcc gctggaactg caaggcgggc aagtgcggct
      421 cgtgcagcgc ggagatcaac ggcaagcccc ggctcatgtg catgacccgg atggatacct
      481 tcaaggaagg tgagccgatc tcggtggccc cgatccggac cttcccgttg atccgggacc
      541 tggtgaccga cgtctcctac aactacgaga aggccaagca ggtgccgccg ctgcggctca
      601 agccgccgga tccggacggc aagtaccgga tgatgcagga ggacgtcgat cgcgggcagg
      661 agttccacaa gtgcatcgag tgcttcctgt gccagaacgt ctgccacgtc atccgcgacc
      721 acgatctcaa gaagcgcttc gcggggccgc gcttcttcat caagatcgcg gggctcgaga
      781 tgcacccgct cgatacccgc gaccgcaccg agttcgtgcg cgagcaggcg ggcgtcgggc
      841 tctgcaacat caccaagtgc tgcaccgagg tctgccccga gcacattcac atcacggaca
      901 acggcatcat cccgctgaag gagcgggcgg ccgacaacct cgacccgatc gtgtggatca
      961 tgcggaagat ccgcgggcac aagaaggcgg cgccctgatc gcatgaagat ccacgagtat
     1021 cagggcaagg cgatcctcaa ggagttcggg gtgcccgtgc ccggcgggga cgtggccgac
     1081 accccggccc aggcccggcg tattgccgaa ggattgggcg gtcgagtagt cgtgaaggcc
     1141 caggtgcacg cgggcgggcg cggcaaggcg ggcggcgtga agctggccga cgacccggcg
     1201 gccgcagagg cggcggccaa gcagatcctc ggcatgatgc tcaagacccc gcagacgccg
     1261 cccgagggca tcagggtgct gaaggtcctg gtggaagagg ctaggccgat cgccaccgag
     1321 ctctatctct cggtcacgct cgaccgcggc cgcggcaccc acgtggtgat ggcctctcag
     1381 gccggcggca tggagatcga ggaggtcgcg gccaagcacc ccgagaagat catccgcgag
     1441 tggacggacc ccgcgctggg gctgcaggcc ttccaggccc gcaacctggc cttcggcctc
     1501 gggctcgcgg gcgaccagtt caaggcgggg gtggccctga tcgtgaacct cttccgggcc
     1561 tatctggaca aggactgctc gctggccgag atcaatccgc tggttattac caccgacggg
     1621 cgtgtattgg ccctggatgc caagctcaac ttcgacgaca acgcgctcta ccggcacaag
     1681 gacgtcgtgg ccctgcgcga tatcaacgaa gagaccccgc tcgacgtgga ggcctcgaag
     1741 tacggcctca actacatcaa gctggacggg agcgtgggct gcatggtgaa cggggccggc
     1801 ctcgcgatgg ccaccatgga catcatcaag ctggcgggcg gagagcccgc caacttcctg
     1861 gacgtgggcg gcggtgcctc gcccgagcag atcgagaacg ccttccgcat cctctcgtcc
     1921 gatccgagcg tgaaggcggt cttcatcaac gtattcggcg gcattctccg agtggatcgg
     1981 ctcgccgagg gcatcatcgc cgcggtgaag aagctggggc tcgagctgcc ggtggtgctg
     2041 cgcgccgagg gcaccaacgt ggagcagggc aagaagatgc tggccgagtc gggcctggcc
     2101 ctcgtgatgg ccagcgacat gggcgacggc gcgcggaagg tggtcgagct ggccaggggc
     2161 ccgaggaaga tcgcattgag gccagataaa tgagcgtgct cgtcgacaag aacacgcgcg
     2221 tgctggtgca gggcatcacc ggcaaggagg gtgccttcca cgccgggcgg tgcaaggagt
     2281 acggcaccca ggtggtgggc ggggtgacgc cgggcaaggg ggggacgacc cacgagggtt
     2341 tcccggtctg gaacacggtg gaggacgctg tcaaggccac cggcgccgac tgcgcgctga
     2401 tcttcgtgcc gccgcccgcc gcggcggacg cgatcatgga agcggccgac gccggcatgc
     2461 cgctcgtcgt ctgcatcacc gagggcgtgc cggtggccga catggtgcgc gtcaaggcct
     2521 tcctcagccg caccacgagc cgcctcatcg gccccaactg cccggggctg atcacgccgg
     2581 gccaggccaa gatcgggatc atgccgggcc acatccacaa ggtcggcaac gtgggcgtgg
     2641 tctcgcgcag cggcacgctg acctatgaag tggtccacca gctcacccag cgcgggctcg
     2701 gccagtcctc ctgcgtgggc atcgggggcg acccggtcaa cggcaccaac ttcatcgacg
     2761 tgctcgagct cttccaggcc gatcccgaga cgacctcgat catgatgatc ggcgagatcg
     2821 gtggcaccgc cgaggaagag gccgccgcct acatcaaggc ccacgtcacc aagccggtgg
     2881 tcggcttcat ctgcgggcag accgcgccgc ccggccgccg catgggccac gccggcgcca
     2941 tcatcgcggg cggcaagggc accgccgcgg agaagatggc cgccatggag gccgccggca
     3001 tcaccaccgt caagagcccc gccgacatgg gcgccaccgt ggcccgcgtg gtgggcaaga
     3061 aagggtaggg acccctccac gatggccgtc cagcgcacac tcaccatcat caagccggac
     3121 gccgtcgcca agggcgtctc ggggcagatc atcgcccgct tcgagcagga gggcctcaag
     3181 gtgctcgccg ccaagctgat ccacctgagc gcggcccagg ccggcggctt ctacatcgtg
     3241 cacaaggagc ggccgttcta cggaagcctc tgcgcgttca tgacccaggg cccgtgcatg
     3301 ccgatcgtgc tcgagggtga gaacgcgatc acgcgcctgc gcgacctgat gggggcgacc
     3361 gatccggcca aggcggcgcc ggggaccatt cgcaaagact ttgcttcctc catcgaggcc
     3421 aacgcggtcc acggctccga ctcgcccgag tcggccgcct tcgagatccc ctacttcttc
     3481 ggcgcgctcg agatcttccc ccgctagccg ggaaggccac gtgcacttcc cggccgtcct
     3541 cgggcgaaag cagcgactcc tcgccggggt ggtcgggatc gggctcggct tcggcgggcc
     3601 ctttctgctg tcggtcgccc tcgttgccag ctccggcgac gcgagcctgc tgatcctgcc
     3661 gctgcccttc ctgggcgcgc tgtgggtgat ccaggggctg gcgccgagcg gctacacgct
     3721 ggaggagggc ggcctgcgcc tcgagcggcg ctgggcccgt cggctcatcc cctacgaccg
     3781 catcaccggc tgcgatcgcg agcgccgccc gatcggcggc ctgctcgccg cgggcgtcaa
     3841 cagcctgttc ggctcccacg ggccgcgctg gaacccgcgc accggctggc actatctcgc
     3901 gatcagcaat accgacgacc tggtgttcgt ccacacgcgc ggcgggctcg tcgtgctctc
     3961 gccgagccgt cccgacgagt tcgtcgccgc gctgtacgcc gagctggccg cgaagcccgc
     4021 gtcatggccg acccgatcgt cgtgaccgcg gacgtccgcg tgccggcctc cgcgctgacc
     4081 gtgcacgcgg tgcgggcctc ggggccgggc ggccagaacg tgaacaaggt cgcgaccaag
     4141 atcgacctgc gcgtggacct ggcggcgctc gagggcctgc ccgacgacgc gcggccgcgc
     4201 ctgcagcgcc tcgcccagaa ccggctcgac gcggacggcc gcctggtggt catcagccag
     4261 gtgacgcgca accaggcgcg caacctggag gacgcgcgcg cccgggtggc cgacctgatc
     4321 cgcgccgccc tcgtggcgcc gcgacgccgc gtgaagaccc ggccgtcggc cggcgcgaag
     4381 caccggcggc tggcgggcaa gaagcgccgg ggcgacgtga agcgctggcg ggcgcggccg
     4441 ggtgaggccg actgagcgcg cccggcgtta gtgaatgccg cccgccacgg tcaggctcac
     4501 caccagcatc gcgagcacga tcgcggcgat cgcggcatag cgccggtcgc ccgcccgcag
     4561 gaacgccggg atcgccagcg cgaccgcgac gatcggcgtg gccatcagcc cgacgaggcc
     4621 gagcgcggtc acggcgaggg gatccaggga ccgccggcgg aggccgtcga gcacctggcg
     4681 cacggaggtg aagacgcccg gcggcgagcc gcccggtggc cggttcaggt agagcgtgtg
     4741 atggtgaaag cccccgtgga ccgcgtagag cgcgcctccg gcgaggatca gcgcgatgcc
     4801 gagcagcccg cccgccagga gcgtgcgcgc gacgaggtgc tcgacggacg gcacgtgtcc
     4861 ggatccgggc cgtcgggctc tgcgggccgc gctcacagca ggtggatgcc cagaccgcgc
     4921 aggatcatcg agatcgcgag atacaccacg atgggcagga acaacttccg cacggtggta
     4981 ttgcgcatcc gcgccatcag gatcgacccc aggtaggcgc cggccagcac tcccatggcc
     5041 accggcgcga cgatcagcgc gttgatgtcg ccccggctga agtagatgcc ggcgctggcc
     5101 gaggcggtaa ggccgatcat gaagttgctg gtggccgtcg agaccttcat cggcagccgc
     5161 atgaggtagt ccatggcgag caccttgaac gcgccgctgc cgatacccag catgccggag
     5221 agcagaccgg ccccgaacat ggcggttaac ccgaagccta cccgtcgcgc tcggtagtcc
     5281 acctcgcgct gcaggtggaa gtcgtagtag cggccttcca gaccgaagcg gatcgcgagc
     5341 ggatcctcgg ggaggcgctc gggcagctcg tcctggagct gccggatgat cgtgaacgcg
     5401 gagtaggaga gtgccaggcc caggatcagc tcgagtgcgc ggaccggcac caccccggcc
     5461 aggaacgcgc cgagcatcgc gccgctgatc gtggccatga ccaggaagag gccgacgcgc
     5521 aggttgctga gcccggtccg caggtacgcg gcggccgcgc cgctcgacgt ggcgatgacc
     5581 gagatgatgc tggcccccat cgcgtagtgg atgtccaccc cgtagaagag ggtcaggatc
     5641 gggatcagca ggatgccgcc gcccaggccc aggatggcgc ccaccacgcc cgcgccgatc
     5701 gacgtgaaga ggaccgcgat cgagaaaacg acgaggctca tggccgggcc atttaggagc
     5761 gggcggcgag gagatcggtc atggtaggag gctatggtag cgttactgcc cccgtgacgg
     5821 gagaacgttc atctcccggt cccgacggga tctgccggtt gaccgatcgg cacacgctaa
     5881 cgcctccacg atagcgccac ggcggttgga ggcgcggcga aggtttcctg ctagaatcca
     5941 ccgcatggcg gcggccatcg acgttcccga tcggttcaac gccgccacct attttctaga
     6001 ccgccacgtc accgagggcc gcggcgcccg caccgccttc cgcttcgccg gccgcgacgt
     6061 cgcgtacggc gagatcgccc tccgcgccaa ccggctcggc aacgcgctcc gggcccgcga
     6121 cgtccagatc gagcagcgcg tgctgctggc cctgccggat cggcccgagt tcgcggaggc
     6181 cttctggggc gccatgaaga tcggcgcggt gccggtcccg gtgagcgacg cgctccccgc
     6241 cgaggagctg gccttcctcc tccaggacag tcgcgcccgc gcggtgatcg cctccgagtc
     6301 ggccgccacc gagatcctca agatccgggc gcagtgcccg gccctggaga gcgtgatcgt
     6361 agtgggcggg cggcggcggg gcgcgctcga gtacgagcgg ctgctagaga aggcctcgcc
     6421 cgagctgacc gcggccgaca cgagtcgcga cgacgtggcg ctctggctct acacctcggg
     6481 ctccaccggc cgccccaagg ccgcggtgca cctgcaccac cacctggtct acgcggcgga
     6541 gctggtgggg cgggggacgt tcgggatcgc gcccgacgac gtgatcttct cggcctcgaa
     6601 gctccacttc gccttcggcc tcggcaactc gctctacttc ccggcgctgg tgggggccgc
     6661 gtcggtgctg gtgccggagc gcctggaggc ggagcaggtg ttcgcgctga tccaggccga
     6721 gcggcccacc atgctgttcg cggtggccac cctctaccgc cggctcctgc aggtggaagg
     6781 cgcggagcgg cggttcgatc tttcgtcgct gcggctctgc gtgtcctcgg gcgaggcgct
     6841 gcccgccggg atcttccacg cctggaagtc gcgcttcggc cacgagctgc tggacgtggt
     6901 gggctccacc gaggcgctgc acgactacat cgccaaccgg ccgggcgagg cgcggccggg
     6961 gtcgagcgga cgggtgatcc cgggctacga ggcgcggctg g
//
LOCUS       DASUFA010000051         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_698103, whole genome shotgun sequence.
ACCESSION   DASUFA010000051 DASUFA010000000
VERSION     DASUFA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698103"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1093)
                     /locus_tag="VFX87_01915"
     CDS             complement(65..1093)
                     /locus_tag="VFX87_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4993688.1"
                     /translation="MIRPSSASGARLAVLLVGLGLVVALVAIGALFLGSARVPPTDVL
                     AVLTGRVKGVDQVVVLSLRLPRILAALLAGGALAVAGAGFQALTRNPLAEPSVLGVSS
                     GAAFGVIVAQVFGLGGGLVEAVGIAACAFAGALVAGVAVYVIASRSGGLPVHTLLLAG
                     VIVGIFFSSAITVPISVLDFDRLGGVVHWLLGNLGPIAPGSLAVFATLTVVGLWLVVG
                     QARPLNLLALGEESAQQLGVDAERLKRRVFAGAALLTSAVVAFVGPIGFVGLIVPQVI
                     RMLLGPDNRVVIPAAVLGGGAFLLLADTLARNVVAPAELSVGVITAFCGAPFFIYLLR
                     ARPPEAGL"
     gene            complement(1090..1995)
                     /locus_tag="VFX87_01920"
     CDS             complement(1090..1995)
                     /locus_tag="VFX87_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX4993689.1"
                     /translation="MTRPVTRLLAVIAMACLLLPQAASALTVTDQTGRRVVLPALPAR
                     IVSLVPSVTETVFTIGAQDRLVGVTDFCDYPPAARQKPRIGGMISPSLEGIVALKPDL
                     VVATTAGNRHETFDQLAQLKIPVYLVNPVAVRDVLDLIVRLGRLTGRSEAADHAAAAL
                     RERIQAVSARVAGRPRPRVLYVLWPDPLIVPARGALVSELIALAGGDSVTADGGQGYP
                     RYSMEAALARNPEVILIASHDATRSPLERSRWERFTQVPAVSAGRIHTVDGNLMHRYG
                     PRIVDGLEVLARLLHPGAFEKAAAP"
     gene            complement(1992..>3029)
                     /gene="cobT"
                     /locus_tag="VFX87_01925"
     CDS             complement(1992..>3029)
                     /gene="cobT"
                     /locus_tag="VFX87_01925"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX4993690.1"
                     /translation="RDPGAELEVRRHLDQLTKPPGSLGRLEELAVRLAGITGICPPPL
                     RDPVIFTLAGDHGVVAQGVSAYPQIVTAQMVENFLRGGAAVNVLARHAGARVVVADLG
                     VGGALPRHPNLRDCKVAPGTADMTRGPAMTAEQCARAIEVGIALVEAERARGADLIGT
                     GEMGIGNTTAASAIVAALTGASPESVTGPGTGVDAAGRRRKVEAIRRALAVNQPDPAD
                     ALDTLAKVGGFEIAGLVGVILAGAASRLPVVLDGFIAGAAALAAVRLRPQARGALLSA
                     HRSAEPGHARVLEALGLEPYLDLGLRLGEGTGAALCIGLARAAVGVLTDMATFKSAGV
                     SDRPDVPGARP"
BASE COUNT          453 a         1138 c         1117 g          321 t
ORIGIN      
        1 aaggcccccg ccgcggcgga cgcgtaggcg aagcccacgc gctcgcattc gacgagcggg
       61 gccgtcatag cccggcctcc ggcgggcgcg cgcggaggag gtagatgaag aacggcgccc
      121 cgcagaacgc ggtgatgacc cccaccgaga gctcggccgg cgcgaccacg ttgcgcgcga
      181 gcgtgtcggc cagcagcagg aaggcgccgc cgcccaggac cgccgccggg atcaccacgc
      241 ggttgtcggg cccgagcagc atccggatga cctggggcac gatcagcccg acgaagccga
      301 tggggccgac gaaggccacc actgccgagg tcaggagcgc ggcgcccgcg aagacacggc
      361 gcttgagccg ctcggcgtcc accccgagct gctgggcgct ctcctcgccc agggccagca
      421 ggttgagcgg gcgcgcctgc cccaccacca gccagagccc gaccactgtc aaggtcgcga
      481 acacggcgag gctgccgggc gcgatgggcc cgaggttgcc gagcagccag tgcaccaccc
      541 cgcccaggcg atcgaagtcc agcaccgaga tcggcaccgt gatggccgag gagaagaaga
      601 tgccgacgat caccccggcg agcagcaggg tgtgcaccgg caggcccccg gaccgcgagg
      661 cgatcacgta gacggccacg cccgcgacca gcgcccccgc gaaggcgcag gccgcgatgc
      721 ccaccgcctc cacgagcccg ccgcccagcc cgaagacctg cgcgacgatc accccgaacg
      781 cggcgccgct cgagaccccg agcaccgagg gctcggccag cgggttgcgc gtgagggcct
      841 ggaagcccgc gcccgccacc gcgagcgcgc cgcccgcgag cagcgcggcc aggatgcgcg
      901 gcaggcgcag gctcagcacc accacctggt ccacgccctt gacgcggccg gtgagcaccg
      961 cgagcacatc ggtgggcggc acccgcgcgc tgccgaggaa gagcgcgccg atcgccacga
     1021 gcgccaccac gagaccgagc ccgaccagca gcaccgcgag ccgcgcgccc gaggcggagg
     1081 acggccggat catggcgcgg ccgccttctc gaaagccccg gggtggagga ggcgcgccag
     1141 cacctccagg ccgtccacga tccgcgggcc gtagcgatgc atcaggttgc cgtccaccgt
     1201 gtggatccgg cccgcgctga ccgccgggac ctgggtgaag cgctcccacc gggaccgctc
     1261 cagcgggctc cgcgtcgcgt cgtggctcgc gatcaggatg acctcggggt tccgcgccag
     1321 ggccgcctcc atgctgtagc gcgggtagcc ctggcccccg tcggcggtca ccgagtcgcc
     1381 gccggccagg gcgatcagct ccgagacgag ggcgccccgc gcgggcacga tgagcgggtc
     1441 cggccagagg acgtagagca cgcgggggcg ggggcggccg gccacgcgcg ccgacacggc
     1501 ctggatgcgc tcgcgcagcg cggccgcggc gtggtccgcc gcctccgagc gaccggtcag
     1561 gcgaccgagc cggacgatca gatccagcac atcgcgcacg gcaactggat tgacgaggta
     1621 aaccgggatc ttgagctgcg ccagttgatc aaacgtttca tgccgattgc ccgccgtcgt
     1681 ggcgaccacg aggtcgggct tgagcgccac gatcccctcg aggctcggcg agatcatgcc
     1741 gccgatcctg ggcttctgcc gggccgcggg cggatagtca cagaaatcgg tgacgccgac
     1801 gagccggtcc tgggccccga tcgtgaacac ggtttcggtc acgctgggca ccagcgacac
     1861 gatccgtgcg ggcagcgctg gcaagacgac gcgcctcccg gtctggtcgg tcaccgtgag
     1921 ggcggacgcg gcctggggta ggaggaggca cgccatcgcg atcacggcga ggagccgggt
     1981 cacagggcgg gtcacggtcg cgctccgggc acgtccgggc gatcggagac gccggcggac
     2041 ttgaaggtcg ccatgtcggt cagcacgccg acggccgccc gcgccaggcc gatgcatagc
     2101 gccgcgcccg tcccctcgcc caggcgcagg ccgagatcga ggtagggctc gaggcccagg
     2161 gcttcgagga cgcgggcatg acccggctcg gccgagcggt gcgcggagag gagcgctccg
     2221 cgcgcctggg gcctgagccg gacggccgcg agtgccgccg ccccggcgat gaagccgtcg
     2281 agcaccaccg gcaggcgcga ggcagctccg gcgaggatga cgcccacgag cccggcgatc
     2341 tcgaagccgc cgaccttggc gagcgtgtcg agcgcgtcgg ccgggtcggg ctggttcacc
     2401 gcgagcgcgc gccggatggc ctcgaccttc cgccgccggc cggccgcgtc cacgcccgtg
     2461 ccggggcccg tgacgctctc cggagaggcc ccggtgaggg cggccacgat tgcgctggcc
     2521 gcggtggtgt tgccgatccc catctccccg gtgccgatga gatccgcgcc gcgggctcgc
     2581 tcggcctcca ccagggcgat gccgacttcg atcgcccgcg cgcattgctc ggccgtcatc
     2641 gcgggcccgc gggtcatgtc cgcggtgccg ggcgccacct tgcaatcccg cagattcggg
     2701 tgccgcggca aggccccccc cacgcccagg tccgccacca cgacccgggc gcccgcgtgg
     2761 cgtgcgagca cgttgaccgc cgctccgccg cggaggaagt tctccaccat ctgtgcggtg
     2821 acgatctgtg gataggcgct caccccctgc gcgaccacgc cgtggtcccc cgcgagcgtg
     2881 aagatcaccg ggtcgcggag cggcggcggg cagatgcccg tgatccccgc gagccgcacc
     2941 gcgagctcct cgagccgccc caggctcccg ggaggcttgg tgagctgatc gaggtggcga
     3001 cgcacctcga gctcggcgcc ggggtcccg
//
LOCUS       DASUFA010000052         5357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1379836, whole genome shotgun sequence.
ACCESSION   DASUFA010000052 DASUFA010000000
VERSION     DASUFA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379836"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1099)
                     /locus_tag="VFX87_01930"
     CDS             complement(110..1099)
                     /locus_tag="VFX87_01930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993691.1"
                     /translation="MAALEIGFIPIEGGHYYREALEEVTRAEELGFDSVWMEEHHSVT
                     DHYWPSPLPVLAGFATRTTRMRLGTDILVAPFYHPVRLAEDAAMLDVMSGGRFVLGIA
                     IGYKPDEFALYGAQLEKRGARFEEQLAIMKALWTQESVSFQGTYYTVMGRLEPRSITR
                     PHPPVWIGGWGDITLRRAATMADNWIPGPTADLPRLLAGKQQFLANRKAAGRPEPVTE
                     WPLTRDVIIADTDREARELAERHIMISYRKEYAGGWRHPFIDASIATDLDGLMKNRFL
                     IGGPEQVIRALRPFVTEYGMTHLICRVFFPGMPHRHIMRELELLSKEVRPAFA"
     gene            complement(1101..1286)
                     /locus_tag="VFX87_01935"
     CDS             complement(1101..1286)
                     /locus_tag="VFX87_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX4993692.1"
                     /translation="MPNITVQWYAGRTDQQKREITAAITDAMVKIGKTTADQVHIVFQ
                     DIEKTNWGVNGKLASDK"
     gene            complement(1310..1639)
                     /locus_tag="VFX87_01940"
     CDS             complement(1310..1639)
                     /locus_tag="VFX87_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993693.1"
                     /translation="MRAPGWLICLVALGLFAGCVSIRIGREFPSPDAHWIVVGRSDRW
                     GLQRMLGEPYQVGFENGDPTWRWLYVQRDGAGAVSKDLIVRFNADGLVRSYSFTSNFP
                     DDLRRLK"
     gene            1794..2816
                     /locus_tag="VFX87_01945"
     CDS             1794..2816
                     /locus_tag="VFX87_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993694.1"
                     /translation="MLFDYDSRVVLSRVDRRAFIGTLAGGLLAAPLVAVAQQTTRVAR
                     VGWLGWVGGAGQPPPEVPLAAFRSGLRDLGWVEGKTLVIEMRSGGREESRALTAELVR
                     LKVDVLVGHGPMVFGAKAQAGALPVVFGINGDPVEAKLVSSIARPGGNVTGITAQALE
                     LAGKRLDLLRQTRPGMARVAVVANEAHPGVQAELRESRVAAQRLGLALQYVPVRAVQD
                     FGSAFDSIAREAAEGLSALPDTLISHEAKAIAEFAAKRRIPTISGWAEFAQAGNLISY
                     GPNLRDFYRHIAVYVDKILKGAKPAELPVEYPTTFELIINLKTAKALGLTIPPSILER
                     ADQVIE"
     gene            2922..4499
                     /locus_tag="VFX87_01950"
     CDS             2922..4499
                     /locus_tag="VFX87_01950"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX4993695.1"
                     /translation="MEAFSPDERRALAPYFTELDGPVFALVNLPEVVKGALFARYSRS
                     AKSLRRLFLDEFLEQPEQPAGAGSIGTTRADRLYERVFSEYGDDSVAQLGGVHLACEG
                     ASNLLTKVLEWGRLMAYLEQSTRYVPYTDRPGGRWKYHVPAELEGHPLRARYVDTLDR
                     AFETYARWIEPMREYWTRRFPKPPADSDAAYRMTIRAKALDSLRGLLPAATRSNVGIF
                     GTGQGYEALLLRMRAHPLQEVRDYADLMHAELRKVIPAFLERLERPERGGVWSRYLAE
                     TAEATAAAAARVLEGIEPAPRPEVTLTDWDPDGEVKVIAAALYSASRLPDDQLLAVAR
                     KLGPEERRQVLDAYVGRRENRRHKPGRAFERTGYRFDVLGDYGAFRDLQRHRLLTIEW
                     QPLSTRHGYVTPDELGDAGVLPEWTRVMEDSAALHDALAAETPAVAPYAAAMAYRVRF
                     YMELNAREAMHMIELRTAPQGHPAYRRICQAMHHLIAEQAGHRALAAAMRYADHSGAE
                     GGRLEAERAAERRRGKS"
     gene            complement(4527..>5357)
                     /locus_tag="VFX87_01955"
     CDS             complement(4527..>5357)
                     /locus_tag="VFX87_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993696.1"
                     /translation="GLLDATGTDITAAGDFVRALGGGRIVVIGSSAPLLSLTGGTHQF
                     GATGNIYRLSGTATAVDPLSGLVLGTAEPIQASGSLFEAVGATATTQRAVRLDVALLQ
                     ASAPLLSLRANGAVAGGLTATSNAIDLTSRAKLNATAPVIALDGSRITVTNASLVNVA
                     GGSYLGVTGNLLSVANGSLISVTGGTLLFAAGGSVVNISGALLSFGGSGSTVTVSNAL
                     CPCTLIGGIPVRLSGGALAANVSIANPIKNAAGNTVSFGTGAAVRVDGATTRVTISGQ
                     "
BASE COUNT          794 a         1852 c         1891 g          820 t
ORIGIN      
        1 ccatggccac gagctcgtcg cggaagtcca tccccatcaa cccgggacgg gcgaacggca
       61 ggacgatcag ccagcgcacg ggcgaagcgt atcacaccga ccccgagcgt caggcgaagg
      121 cgggccgcac ttccttggac agcagctcca gctcgcgcat gatgtgacgg tgcggcatgc
      181 cggggaagaa gacccggcag atgagatggg tcatgccgta ctcggtgacg aagggccgga
      241 gcgcgcggat gacctgctcg ggcccgccga tgaggaagcg attcttcatc agcccgtcga
      301 gatcggtcgc gatggaggcg tcgatgaacg ggtgccgcca gccccccgcg tactccttgc
      361 ggtacgagat catgatgtgc ctctcggcca gctcgcgcgc ttcccggtcg gtgtcggcga
      421 tgatgacgtc tcgcgtgagc ggccactcgg tcaccggctc cggccggccc gccgccttcc
      481 ggttggccag gaactgctgc ttgccggcga gcaggcgggg caggtccgcg gtgggccccg
      541 ggatccagtt gtcggccatg gtggcggcgc ggcgcagggt gatgtcgccc cacccgccga
      601 tccagaccgg aggatgcggg cgagtgatgg atctgggctc gagccggccc atcaccgtgt
      661 agtaggtgcc ctggaaggac accgactcct gcgtccagag ggccttcatg atcgcgagct
      721 gctcctcgaa tcgcgcgccg cgcttctcga gctgggcgcc gtagagagcg aactcgtccg
      781 gcttgtagcc gatcgcgatg cccagcacga agcgcccgcc cgacatcacg tcgagcatgg
      841 ccgcgtcctc ggcgagccgg accggatggt agaacggcgc caccaggatg tcggtgccga
      901 ggcgcatccg ggtggtccgc gtcgcgaagc ctgccagcac cgggaggggc gacggccagt
      961 agtgatcggt gaccgagtgg tgctcctcca tccagaccga gtcgaagccc agctcctcgg
     1021 cgcgcgtgac ctcctcgagc gcctctcggt agtagtgccc gccctcgatg gggatgaagc
     1081 cgatctccaa agcggccact ctacttgtcg ctggccagct tgccgttcac gccccagttc
     1141 gtcttctcga tgtcctggaa cacgatgtgc acctggtccg cggtggtctt gccgatcttg
     1201 accatcgcgt cggtgatggc ggcggtgatc tcgcgcttct gctggtcggt gcggccggcg
     1261 taccactgga cggtgatgtt gggcatggga gcctcctcgg tggtcggggt tacttgagtc
     1321 gccgcagatc gtcggggaag ttggaggtga acgagtagga tcggacgagc ccgtcggcgt
     1381 tgaagcgcac gatgaggtcc ttgctgaccg cgcccgcgcc gtcccgctgg acgtacagcc
     1441 atcgccaggt cggatcgccg ttctcgaagc cgacctggta gggctcgccg agcatccgct
     1501 ggaggcccca gcggtcgctc cggccgacca cgatccagtg cgcgtcgggc gaggggaact
     1561 cgcgtccgat cctgatcgag acgcagccgg cgaagaggcc gagcgcgacc agacagatca
     1621 gccacccggg cgcgcgcatg gcgcggagta tgaatccgcc caggtatttt gtcaacgatt
     1681 cgcgacgttg cacgtcacgg tacatgaaaa ccactgctga ttatcaacag ggctcgcgcc
     1741 ccgcctttct catctcatga gtagggatac tctcaactca actggctgac gtggtgttgt
     1801 tcgactacga tagccgcgtc gtgctctcac gagtggatcg gcgggcgttc atcggcacct
     1861 tggcgggcgg cctcctcgcc gcgccgctcg ttgccgtggc gcagcagacc acgagggtcg
     1921 cgcgagttgg ctggctgggc tgggttggag gagccgggca gccaccgccc gaggtgcctc
     1981 tcgcggcctt tcggtccggc ttacgcgacc tcggatgggt ggaaggcaag accctcgtca
     2041 ttgagatgcg tagcggtggc cgagaggagt cccgcgcgct cacggcggaa ctggtccggc
     2101 tgaaggtcga cgttctcgtt gggcatggcc ccatggtctt cggggccaag gcccaggctg
     2161 gcgcgcttcc cgtggtgttc ggcatcaacg gcgaccccgt tgaggcaaag ctcgtctcga
     2221 gcatcgcgcg acctggcggg aacgtcaccg gtatcactgc gcaggctctg gaactggcag
     2281 gcaagcgtct cgacctgctc cggcagaccc ggcccggcat ggcccgcgtc gcggtcgtgg
     2341 ccaacgaggc gcaccccggg gtgcaggccg agctccgaga gtcgcgggtc gctgcccagc
     2401 ggctcgggct ggccttgcag tatgtgccgg tgcgcgcggt ccaggatttc ggcagcgcgt
     2461 tcgacagtat cgcccgagag gcggctgagg gcctcagcgc cctccctgac accctcatca
     2521 gccacgaagc caaggccatc gccgagtttg ccgccaagcg gcgtatcccg accatctcgg
     2581 ggtgggccga attcgcgcag gccgggaatc tcatcagtta cggacctaac ttgcgcgact
     2641 tctaccgcca catcgccgtc tacgtcgaca agattctcaa gggtgccaag cctgccgagt
     2701 tgcctgtcga gtacccgacc acgttcgaat tgatcatcaa cctcaagacc gccaaggccc
     2761 tcggcctgac aatcccgccc tcgattctgg agcgggcgga tcaggtgatc gagtagcggc
     2821 tgagggcctg gcgctgccgg gtccgcgccc tgcctctcgc tatcacgctt cacttgttgc
     2881 ccggacgcgt cgcgcttgac gggaggtgct accatgccgg cgtggaagcg ttcagccccg
     2941 acgagcggcg cgccctggcg ccctacttca ccgagctcga cggccccgtc ttcgccctcg
     3001 tcaacctgcc ggaggtggtc aagggcgccc tgttcgcgcg ctactcccgc tccgccaagt
     3061 ccctgcggag gctcttcctc gacgagttcc tggagcagcc ggagcagccc gcgggcgcgg
     3121 gctccatcgg gaccacccgc gccgaccggc tctacgagcg cgtcttcagc gagtacggcg
     3181 acgactcggt cgcccagctc ggcggcgtgc acctggcctg cgagggcgcg tccaatctcc
     3241 tgaccaaggt gctcgagtgg gggcggctca tggcctatct cgagcagtcc acccgctacg
     3301 tgccctacac ggaccggcca ggcggccgct ggaagtatca cgtgccggcg gagctcgagg
     3361 gccatccgct gcgggcgcgc tacgtggaca cgctcgatcg cgcgttcgag acctacgcgc
     3421 gctggatcga gccgatgcgc gagtactgga cgcggcggtt cccgaagccg cccgcggaca
     3481 gcgacgccgc ctatcgcatg acgatccgag ccaaggcgct ggacagcctg cgcgggctcc
     3541 tgcccgcagc gacgcggtcg aacgtgggca tcttcggcac cggccagggc tacgaagccc
     3601 tcctgctccg catgcgcgcg caccccctgc aggaggttcg cgactacgcc gatctcatgc
     3661 acgccgagct gcggaaggtg atccccgcct tcctggagcg cctcgagcgc ccggagcggg
     3721 gcggggtctg gtcgcgctat ctcgcggaga ccgcggaggc caccgcggcg gcggcggccc
     3781 gcgtgctcga gggcatcgag cccgcgcccc ggccggaggt cacgctcacc gactgggatc
     3841 cggacggcga ggtgaaggtg atcgcggccg cgctctactc ggcctcccgc ctgccggacg
     3901 accagctcct ggcggtggcg cgcaagctcg gcccggagga gcgacgacag gtcctggacg
     3961 cctacgtcgg ccggcgggag aaccggcgcc acaagcccgg gcgcgccttc gagcgcaccg
     4021 gctatcgctt cgacgtgctg ggcgactacg gcgccttccg cgacctccag cggcaccggc
     4081 tgctcacgat cgagtggcag ccgctctcga cccggcacgg ctacgtgacg ccggacgagc
     4141 tcggcgacgc gggcgtgctc ccggagtgga cgcgcgtgat ggaggactcc gccgcgctcc
     4201 acgacgcgct ggccgccgag acgcccgcgg tcgcgcccta cgcggcggcc atggcctacc
     4261 gggtgcgctt ctacatggag ctgaacgcgc gcgaggccat gcacatgatc gagctgcgca
     4321 ccgcccccca ggggcatccg gcctaccggc gcatctgcca ggcgatgcac catctcatcg
     4381 cggagcaggc cgggcatcgt gcgctcgcgg cggccatgcg ctacgcggat cactcgggtg
     4441 cggagggcgg acggctcgag gcggagcgcg ccgcggagcg ccggcgcggg aagagctgat
     4501 cggcggcggg ttcccgccgc ccggacctac tgtccgctga tcgtgacccg ggtggtcgcg
     4561 ccgtccactc ggacggcggc gccagtcccg aaggacacgg tattgcccgc cgcgttcttg
     4621 atcgggttcg cgatggacac gttggcggcc agcgcgcctc cggagagccg caccgggatg
     4681 ccgccgatga gcgtgcacgg gcacagcgcg ttgctcaccg tgaccgtgct gccggacccg
     4741 ccgaaggaga gcagcgcccc gctgatattg accaccgagc cgcccgctgc gaagagcagg
     4801 gtcccgccgg tgaccgagat caggctgccg ttggccacgc tcaggagatt gcccgtcacg
     4861 ccgagatagc tgccgcccgc cacgttgacc agcgaggcgt tggtgacggt gatccggctg
     4921 ccgtccagcg cgatgaccgg cgcggtcgcg ttcagcttcg cgcgcgacgt gagatcgatc
     4981 gcgttgctgg tggcggtgag cccgcccgcc accgcgccgt tggcgcgcag gctcagcagg
     5041 ggcgcgctgg cctggagcag ggccacgtcc aggcgcaccg cccgctgggt ggtcgcggtg
     5101 gcgccgaccg cctcgaagag ggagccgctg gcctggatcg gctccgcggt gccgagcacc
     5161 agcccgctga gcggatcgac cgcggtggcg gtgccggaga ggcggtagat gttgccggtc
     5221 gcgccgaact ggtgcgtgcc gccggtgagc gagagcagcg gcgcgctcga gccgatcacc
     5281 acgatgcgcc cgccgcccag cgcccgcacg aagtcgcccg ccgcggtgat gtcggtgccg
     5341 gtggcgtcga ggaggcc
//
LOCUS       DASUFA010000053         7430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_11986, whole genome shotgun sequence.
ACCESSION   DASUFA010000053 DASUFA010000000
VERSION     DASUFA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11986"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VFX87_01960"
     CDS             complement(<1..1417)
                     /locus_tag="VFX87_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4993697.1"
                     /translation="MSLSARVVIVGGGIVGTSVAYHLAQLGWRDVVLLEQGSLSCGTT
                     WHAAGLVGQLRSSSNLTRLIRYSADLYSRLEALTGQSTGWKRCGSVSVARTAERMIQL
                     KRNAAMAAAYGVEAHVISREEAAARYPLMRIDDLVGAVWIPGDGKANPADITQALARG
                     ARAGGARLMEGVKVTGVNVARGAVTGVETSAGPIATEILVNCAGMWARELGRMSGVTI
                     PLHACEHMYIVTQPIQGVASDLPVLRDPDGHIYVKEEVGGLLMGGFDPWAKPWGVEGI
                     PEGFAFGTLKEDWGKFDILMRNALVRLPALETAPVRTFLNGPESFTPDNYFILGEAPE
                     VRRYYVGAGFCSGGIAAAGGAGKALAEWIVEDRAPMDLWPADIRRFAPLHGNRRFLRE
                     RVSEIVGVHYDIAFPNRELTSGRGLRRSPLHERLRERRACFGAKMGWERANWFAPEGV
                     APEAVYSFGRQNWFPFSAAEHR"
     gene            complement(1439..2674)
                     /gene="phnA"
                     /locus_tag="VFX87_01965"
     CDS             complement(1439..2674)
                     /gene="phnA"
                     /locus_tag="VFX87_01965"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEX4993698.1"
                     /translation="MTTRGGAPITVNGRRYAWPDRPLVVVCVDGSEPDYIRRAIAAGA
                     MPYLAAALPGGTDRLADCVVPSFTNPNNLSIVTGVPPSAHGICGNYFFDREAGAEVMM
                     NDPRYLRCGTILAAFAREGARVAVVTAKDKLRRLLGHEMRGICFSAEKANETTEAEHG
                     VTDGLKLVGLPLPSVYSADLSEFVFAAGVKLMESARPDIMYLSTTDYVQHKHPPGSAD
                     ANEFYGMMDGYYAKLAGLGATLAITADHGMNAKTDDAGRPRVIYLQDHLDQWVGLGRA
                     RVILPITDPYVVHHGALGSFATVYLPEGADTEDLRRRLAGLAGMEVVLDRAAACRRFE
                     LPPDRVGDLVAVSERHVVLGTSLARHDLSGLDAPLRSHGGITEQVVPLLLNRATKGVP
                     GNRRLRNFDLFDLALNHVQ"
     gene            complement(2671..3921)
                     /locus_tag="VFX87_01970"
     CDS             complement(2671..3921)
                     /locus_tag="VFX87_01970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4993699.1"
                     /translation="MNLSVAILSVHTCPLAALGGKQTGGMNVYVRQTARELGLLGVRV
                     DVFTRSQNARIPRIVELGPGARVIHLPAGPEAPMPREAVHGHLDEFAAGAEAFAREQG
                     LRYDLIHSHYWLSGVAGLRLRERWGAPLVHMFHTLGRLKNEVAETPGELEPALRIDEE
                     TRIVAEADRIVAANVVERAHLVWYYGARSERVAVIPCGVDTELFQPMDPAKARDLLEL
                     PPDPLLLYVGRLTPIKGLDTLLEAMVAIPEPAYLLVVGGEHDEPDGGHGTALRAKVTA
                     LGLERRVRFLRAQPQRRLRLFYAAADVTVMPSHYESFGMVALEAMACGSPVVASRVGG
                     LTTTVQDGVTGRLVPEGDPAALAAAIVPLLGSAEGRRLGQQATRWAADHRWPCVAESV
                     CRLYSELRPAAREHLPHTPCRSWR"
     gene            complement(3918..4901)
                     /locus_tag="VFX87_01975"
     CDS             complement(3918..4901)
                     /locus_tag="VFX87_01975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4993700.1"
                     /translation="MKIDALPGLAALDEGRWNALLDRSRLPSLFLTWQWQTEWSRAFA
                     ADRPQQILTATDADGSLAGVLPLYEDGPGRQRIIGGVDVSDYLDLIAVAGREEEIWQA
                     LLQHRAGQPGEWDLHGIRAASVTATALPALAPAHGLRATVERQDRCPVLALPKTWDEY
                     LAKLSGKDRHELRRKMRRLERELPGATVRAHAEPAGWDEAMTRFLTLHRLSKVGKARF
                     MDERMERFFRSATAILAAKGWARLWFLEHEGAAVAAFICLEYAGSVGLYNSGFDPARA
                     QLAPGIVLLAHVIQDAIERGIPTFDFLRGEEPYKYGFGPSPEDVFNVRVGP"
     gene            complement(4898..5290)
                     /locus_tag="VFX87_01980"
     CDS             complement(4898..5290)
                     /locus_tag="VFX87_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX4993701.1"
                     /translation="MPIYEYECQGCHRRVSLLIRTLSAAEAPRCPRCGSVALSRLMSR
                     FATVKSEDARLDSLSDPSSFGDLDENDPQSVARFVKKMGHEMGEDLGEDLDAAMDEAM
                     GEEGGAGADDGALMDPGGGGDDAGEPSA"
     gene            complement(5360..6400)
                     /locus_tag="VFX87_01985"
     CDS             complement(5360..6400)
                     /locus_tag="VFX87_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX4993702.1"
                     /translation="MPKSSAGSSSPPLIRYHPGGPVATEYRVGLCAWQDKSMVEEGSF
                     YPIKSMKAEERLWWYSRFFDCVEVNSTFYAPLSAQNAVLWAKRTPPGFLFSVKAYALL
                     TGHHLDAERVPPPLAALLPASARPNARGQIENGVFGEDAREWAFAAFREGLQPLADAG
                     KLGYVLFQMAPWVKYGDRALEYLAGLPARLPGTTIAVEFRDASWLPRHTEDTLTMLSE
                     RGLAYVSVDAPRTPASVDTAVALTAPTAVIRLHGRNREGFMKQLQGRAPSVAEKYGYL
                     YDRAELAGIVARGRALEGQARRVYFKLNNNVGDAPAINGEDIKELLGLETADRAAVLQ
                     EWRSRRKAPTRP"
     gene            complement(6378..>7430)
                     /locus_tag="VFX87_01990"
     CDS             complement(6378..>7430)
                     /locus_tag="VFX87_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4993703.1"
                     /translation="RRGDEVLREHFPGADSNRILVVVRYPDGTPLTAERVGGLYDLSR
                     WLAARPNVARVESLVDLDPSVTRAQYQQLATAPAEMRPPGVDQVLRQTVGGRLALLVV
                     STPLKPSSEEARALVQEIRASHPAVGGEVLVTGQTAFDLDIIHLVTRHAPLTVGLVML
                     ATYVVLFLLLGSVLLPLKAVIMNLLSISASYGALVWIFQEGHLASWLHFTPGPIQTAT
                     PLIMFCFVFGLSMDYEVLLLSRVREEYERSGDTTRAVAEALESTGRLITGAAAIMAAV
                     FFGFATARSVIIQAVGIGIGLAVVIDASIVRALLVPATMRLMGRWNWWAPAPLVSLHR
                     RLGLAERSADAEVVRR"
BASE COUNT         1078 a         2684 c         2654 g         1014 t
ORIGIN      
        1 cccggtgctc ggccgcggag aacgggaacc agttctggcg accgaaggag tagacggcct
       61 ccggcgcgac cccctcgggc gcgaaccagt tggcgcgctc ccagcccatc ttcgccccga
      121 agcacgcgcg gcgctcccgc aaccgctcgt gcagcggcga gcggcgcagc ccgcgcccgc
      181 tcgtgagctc gcggttcggg aacgcgatgt cgtagtgcac gcccacgatc tcgctgaccc
      241 gctcgcgcag gaagcgccgg ttgccgtgca gtggcgcgaa gcggcgtatg tcggccggcc
      301 agagatccat cggcgcgcgg tcctcgacga tccactccgc cagcgccttc cccgcgccgc
      361 ccgcggcggc gataccgccc gagcagaagc ccgcgcccac gtagtagcgg cgcacctcgg
      421 gggcctcgcc caggatgaag tagttgtcgg gggtgaagga ctcgggcccg ttcaggaagg
      481 tgcgcacggg agcggtctcg agcgcgggca gccgcacgag cgcgttgcgc atcaggatgt
      541 cgaacttgcc ccagtcttcc ttgagcgtcc cgaacgcgaa gccctccgga atgccctcga
      601 cgccccacgg cttggcccac ggatcgaaac cgcccatgag gaggccgccc acctcttcct
      661 tcacgtagat gtggccgtcg gggtcgcgca gcaccggcag gtccgaggcc accccctgga
      721 tgggctgggt cacgatgtac atgtgctcgc aggcgtgcag cgggatcgtc acgccgctca
      781 tccgcccgag ctcgcgcgcc cacatgcccg cgcaattgac caggatctcc gtcgcgatgg
      841 ggcccgcgga ggtttcgacg ccggtgaccg cgccccgcgc cacgttgacg ccggtgacct
      901 tgaccccctc catcagccgc gccccgcccg cgcgtgcccc gcgggccagc gcctgggtga
      961 tgtcggccgg gttggccttg ccgtcgccgg ggatccacac cgcgccgacc agatcgtcga
     1021 tgcgcatcag cgggtagcgc gccgccgcct cctcccggct gatcacgtgg gcttccactc
     1081 catacgcagc cgccatcgcg gcgttgcgct tgagctggat catgcgctcg gcggtccgcg
     1141 cgacgctcac gctgccgcag cgcttccacc cggtggactg gccggtcagc gcctcgaggc
     1201 ggctgtagag atccgcgctg tagcggatca gccgggtgag attgctggag gaccggagct
     1261 gcccgaccag gccggccgcg tgccacgtgg tcccgcagga gaggctcccc tgctcgagca
     1321 gcaccacatc ccgccagccg agctgcgcca ggtgatatgc gacgctcgtg ccgacgatgc
     1381 cgccgccgac gattacgacg cgcgcgctaa ggctcatggg ccgatggcgg acaaccgctt
     1441 actgcacgtg gttcagggcc aggtcgaaga gatcgaagtt acggaggcgg cggttgccgg
     1501 ggacgccctt ggtcgcgcgg ttcaggagca gaggcacgac ctgctcggtg atgccgccgt
     1561 gggagcggag cggggcgtcg aggcccgaga gatcgtggcg ggccaggctg gtgccgagca
     1621 cgacgtggcg ctcggacacc gcgaccaggt cacccacgcg gtcgggcggc agctcgaagc
     1681 ggcggcaggc ggcggcccgg tccagcacca cctccattcc ggccagcccg gcgaggcgcc
     1741 ggcgaagatc ctccgtgtcc gcgccctcgg gcaggtagac ggtggcgaag gagcccagcg
     1801 ccccgtggtg cacgacgtag ggatccgtga tgggcaggat gacccgcgcc cgtccgaggc
     1861 ccacccactg atccaggtga tcctggaggt agatgacccg cggccggccc gcgtcgtcgg
     1921 tcttggcgtt catgccgtgg tcggcggtga tggcgagggt ggcgccgagg ccggccagct
     1981 tcgcgtagta gccgtccatc atgccgtaga actcgttggc gtccgccgag ccgggcggat
     2041 gcttgtgctg cacgtagtcg gtggtggaga gatacatgat gtcgggtcgc gccgactcca
     2101 tcagcttcac gcccgccgcg aacacgaact cggagagatc ggcgctgtac acggagggca
     2161 gcggcaggcc gaccagcttg agcccgtccg tcaccccgtg ctcggcctcc gtggtctcgt
     2221 tggccttctc ggccgagaag cagatgccgc gcatctcgtg gcccagcagg cgccgcagct
     2281 tgtccttcgc ggtcaccacc gccacccgcg cgccttcacg ggcgaacgcg gcgaggatcg
     2341 tcccgcagcg caggtagcgg ggatcgttca tcatgacctc cgcgcccgcc tcgcggtcga
     2401 agaagtagtt gccgcagatg ccgtgggccg agggcggcac gccggtcacg atggagagat
     2461 tgttgggatt cgtgaagctc ggcaccacgc agtcggccag gcgatcggtc ccgcccggca
     2521 gcgccgcggc caggtacggc atcgccccgg ccgcgatggc gcgccggatg tagtccggct
     2581 cgctcccgtc cacgcacacc acgaccaggg ggcgatccgg ccacgcgtag cgccggccgt
     2641 tcaccgtgat gggggctcca ccgcgagtcg tcatcgccag ctcctgcacg gggtgtgagg
     2701 gagatgctcc cgggccgcgg ggcgcagctc ggagtagagg cggcagaccg actccgccac
     2761 gcacggccag cggtggtcgg cggcccagcg cgtggcctgc tgaccgagcc gccgcccctc
     2821 cgcgctgccg agcaagggca cgatggcggc ggccagcgcg gccggatcgc cctcggggac
     2881 caggcggccg gtcaccccgt cctgcaccgt ggtggtcagc ccgccgacgc gcgaggcaac
     2941 caccgggctg ccgcaggcca tggcctcgag cgccaccatg ccgaaggatt cgtagtgcga
     3001 cggcatcacg gtgacgtcgg cggccgcgta gaacaggcgg agccgccgct ggggctgcgc
     3061 gcgcaggaag cgcacgcggc gctccaggcc gagcgcggtc accttcgccc gcagcgcggt
     3121 gccgtggccc ccgtcgggct cgtcgtgctc gccgccgacg acgaggaggt aggcgggctc
     3181 gggaatggcg accatggcct ccagcagcgt gtcgaggccc ttgatcggcg tgagccgccc
     3241 cacgtagagc agcagcggat ccggcggcag ctcgagcagg tcgcgggcct tggccggatc
     3301 catcggctgg aagagctccg tgtccacgcc gcaggggatc accgcgaccc gctcgctgcg
     3361 cgcgccgtag taccagacga ggtgcgcccg ctccaccacg ttggcggcca cgatgcgatc
     3421 ggcctccgcc acgatgcggg tctcctcgtc gatgcggagc gcgggctcga gctcacccgg
     3481 ggtctcggcc acctcgttct tgagccgccc cagggtgtgg aacatgtgca cgaggggcgc
     3541 gccccagcgc tcgcgcagcc gcagccccgc cacgcccgac agccagtagt gcgagtggat
     3601 gaggtcgtag cggagcccct gctcccgcgc gaacgcctcc gctcccgccg cgaactcgtc
     3661 gagatggcca tggaccgcct cgcgcggcat cggcgcctcc gggcccgcgg gcaggtggat
     3721 cacgcgggcg cccgggccca gctcgacgat ccggggaatc ctggcgttct gcgagcgcgt
     3781 gaacacgtcc acgcgcacgc ccaggagacc cagctcgcgc gcggtctgcc gcacgtagac
     3841 gttcatgccg ccggtctgct tgccgccgag ggcggcgagc gggcaggtgt gcacggagag
     3901 gatcgcgacg gagaggttca cgggccgacc cggacattga agacgtcctc cggcgacggg
     3961 ccgaagccgt acttgtacgg ctcctcgccg cgcaggaagt cgaaggtggg gatgccgcgc
     4021 tcgatggcgt cctggatgac gtgggcgagc agcacgatgc cgggcgcgag ctgcgcgcgc
     4081 gcggggtcga agcctgagtt gtagaggccg acgctccccg cgtactcgag gcagatgaac
     4141 gcggccaccg ccgcgccctc gtgctcgagg aaccagagcc gcgcccagcc cttcgcggcc
     4201 aggatcgcgg tggcggagcg gaagaagcgc tccatgcgct cgtccatgaa gcgcgccttg
     4261 cccaccttgg agaggcggtg gagcgtgagg aagcgcgtca tggcctcgtc ccagccggcg
     4321 ggctccgcgt gcgcgcgtac cgtcgccccg gggagctcgc gctcgaggcg ccgcatcttg
     4381 cgccggagct cgtgccggtc cttgccggag agcttcgcga ggtactcgtc ccacgtcttg
     4441 ggcagcgcca gcaccgggca gcggtcctgc cgctccaccg tggcccgcaa cccgtgagcc
     4501 ggcgcgagcg cgggcagcgc ggtggcggtg accgaggccg cgcggatacc gtgcagatcc
     4561 cactcgcccg gctgcccggc gcgatgctgg agcagcgcct gccagatctc ctcctcgcgt
     4621 ccggccaccg cgatcaggtc gaggtagtcg gagacgtcca ccccgccgat gatccgctgc
     4681 cgcccgggcc cgtcctcgta gagcggcagc acgccggcca ggctgccgtc cgcgtcggtc
     4741 gcggtcagga tctgctgggg gcggtccgcc gcgaaggccc ggctccattc ggtctgccac
     4801 tgccaggtga gaaacagcga gggcaggcgc gagcggtcga gcagggcatt ccagcgtccc
     4861 tcgtccaggg ccgcgaggcc gggcagcgcg tcgatcttca cgccgacggc tcccctgcgt
     4921 cgtccccgcc tccgcccgga tccatgagcg cgccatcgtc ggctccggcc ccaccctcct
     4981 cacccatcgc ctcgtccatg gcggcgtcga ggtcctcgcc caggtcctcg cccatctcat
     5041 ggcccatctt cttcacgaac cgggccacgc tctgggggtc gttctcgtcg agatcgccga
     5101 agctggaggg gtcggagagg gagtcgaggc gggcgtcttc agacttgaca gtggcgaagc
     5161 gggacatcag ccgcgacagc gcgacgctgc cgcagcgcgg acatcgcggc gcctcggccg
     5221 cggacagggt gcggatcaga aggctgaccc gccggtgaca cccctggcac tcgtactcgt
     5281 aaatcggcac gcgcctgccc tcccgccccg tttggatgcg ccattctatc acgcggattc
     5341 cggggcggat tcggggcctt caggggcgag tcggggcttt gcggcggctc cgccattcct
     5401 gcaggacggc cgcgcgatcc gcggtctcga gccccagcag ctccttgata tcctcgccgt
     5461 tgatggcggg cgcatccccg acattgttgt tgagcttgaa gtagacccgt cgggcctgcc
     5521 cctccagggc gcgcccgcgg gccacgatcc cggccagctc ggcgcggtcg tagaggtagc
     5581 cgtacttctc ggccacgctg ggggcccggc cctggagctg tttcatgaag ccctcccggt
     5641 tgcgcccgtg cagccggatg accgcggtgg gcgcggtgag cgccaccgcc gtgtccaccg
     5701 aggcgggcgt ccgcggcgca tccaccgaga cgtaggccag cccgcgctcg ctcagcatcg
     5761 tgagcgtgtc ctcggtgtgc cgcggcagcc aggaggcgtc gcggaactcc accgcgatcg
     5821 tggtcccggg caggcgggcg ggcaggcccg cgagatactc gagcgcccgg tctccatact
     5881 tcacccacgg cgccatctgg aagagcacgt agccgagctt gcccgcgtcg gccagcggct
     5941 gcaggccctc ccggaaggcg gcgaaggccc actcgcgcgc gtcctcgccg aacacgccgt
     6001 tctcgatctg gccccgcgcg ttgggccggg cggaggcggg cagcagcgcg gcgagcggcg
     6061 gcggcacgcg ttccgcatcc aggtgatgcc cggtgagcag cgcgtaggcc ttcacgctga
     6121 agaggaagcc gggcggcgtc cgcttcgccc agagcaccgc gttctgcgcg gagagcggcg
     6181 cgtagaacgt ggagttgacc tcgacgcaat cgaagaagcg gctataccac cagagccgct
     6241 cctccgcctt catgctcttg atcgggtaga acgaaccctc ctccaccatg ctcttgtcct
     6301 gccacgcgca gagaccgacg cggtactccg tcgcgacggg gccgccgggg tggtagcgga
     6361 tcagcggagg ggacgagcta ccggcggacg acttcggcat cggcgctgcg ctcggccaaa
     6421 cccaggcggc ggtggagcga gaccagcggc gcgggcgccc accagttcca ccgccccatg
     6481 agccgcatgg tggcgggcac cagcagggcg cgcacgatgc tggcgtcgat caccaccgcg
     6541 agcccgatgc caatgcccac cgcctggatg atcaccgagc gcgcggtcgc gaacccgaag
     6601 aacaccgcgg ccatgatggc ggccgcgccg gtgatgagcc gcccggtgga ttcgagcgcc
     6661 tcggccaccg cccgcgtggt gtctccgctg cgctcgtact cctcgcgcac ccggctcagc
     6721 agcaggacct cgtagtccat cgagagcccg aacacgaagc agaacatgat gagcggcgtg
     6781 gcggtctgga tcgggcccgg ggtgaagtgc agccagctcg cgagatgccc ctcctggaag
     6841 atccagacca gcgcgccgta ggaggcgctg atggacagga ggttcatgat caccgccttg
     6901 agcggcagca gcaccgagcc cagcagcagg aacagcacca cgtacgtggc gagcatgacc
     6961 agccccaccg tgagcggcgc gtgccgggtg accaggtgga tgatgtcgag gtcgaacgcg
     7021 gtctggccgg tcaccagcac ctcgccgccc accgcgggat gggaggcccg gatctcctgg
     7081 accagcgcgc gcgcctcctc gctcgacggc ttgagcgggg tcgacaccac cagcagggcc
     7141 aggcggccgc ccaccgtttg ccgcaggacc tggtccactc cgggcgggcg catctccgcg
     7201 ggggcggtgg cgagctgctg atactgcgcg cgggtcacgc tcggatcgag atcgaccagg
     7261 ctctccaccc gcgccacgtt cggccgcgcg gcgagccagc ggctcaggtc gtagaggccg
     7321 cccacccgct cggcggtgag cggcgtcccg tccgggtagc gcaccaccac caggatgcgg
     7381 ttgctgtcgg ctcccgggaa gtgctcgcga aggacctcgt ccccccgacg
//
LOCUS       DASUFA010000054        17925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_929195, whole genome shotgun sequence.
ACCESSION   DASUFA010000054 DASUFA010000000
VERSION     DASUFA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 17925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17925
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929195"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /gene="groL"
                     /locus_tag="VFX87_01995"
     CDS             complement(<1..1537)
                     /gene="groL"
                     /locus_tag="VFX87_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701149.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX4993704.1"
                     /translation="MAHKQVLFRSEARERILRGVAALADAVRVTLGPKSKCVLLEKRF
                     GAPVVCNDGVTIAKEMELKDPVENLGAQMIRQAAERTGDAVGDGTSTSTILAHAIFAD
                     GVRNVVAGASAVDLKKGLDRGVRAAVEALKLLSQPVKSRLEKAQVATISAHNDQSIGE
                     MVADAMEKVGNEGVITVEESKTTETQLEVVEGLQFDRGYLSPYFITDPEKMEAVLEDT
                     LVLLSDRKIGVMKDLVPILEQAARAGRSILVIAEDVEGEALATLVVNKIRGSLKCVAV
                     KAPGFGDRRKEMLEDMGILTGGRVLTEEIGIKLEALSIEDLGRATRVVVDKDTTTIIG
                     GGGDKARIDGRKQQIRAQIDKTTSDYDREKLQERLAKLSGGVAVVRVGAPSESEMKSK
                     REALDDAINATKAAVAEGIVPGGGLALLRAIDAVEREEAQCEGDERTGLQILRRALEA
                     PTRQIAENSAADGGVVVDKMLASAANVGFDAAVKKFVDLYEAGIVDPAKVVRLGLENA
                     VSVA"
     gene            complement(1556..2077)
                     /locus_tag="VFX87_02000"
     CDS             complement(1556..2077)
                     /locus_tag="VFX87_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX4993705.1"
                     /translation="MHERLEELLGVRGVRYELVPHLEAFTAQEEAAKAHVSGWSWAKT
                     VVVKDPVGLALAVLPASCTADLDRLKGLIGRGEIRLASVEEILAGLPGCALGATPPFG
                     GLFGIPTVVDESLVEQRDIVLPAGDHRTAIRMRTADYLRLAEARSGHFAIHEAQAFPV
                     HPHPRGRGARPRP"
     gene            complement(2210..3406)
                     /locus_tag="VFX87_02005"
     CDS             complement(2210..3406)
                     /locus_tag="VFX87_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX4993706.1"
                     /translation="MSLVIESIGGTPVPRRRIELVERKGLGHPDTICDSVVEAASVAL
                     GRLYLERAGVIAHYNIDKALLVAGQCVKRFGGGEVTRPMELIIGDRATDVLDGRPLPV
                     VETVRHAVDEWVRANLPGVRAGKDLVTRVALAPGSEELRRVYTEGETEIASNDTSGAS
                     GYAPLSPTEELVLAIERHLNSGAFKAAFPDTGQDVKVMGVRHDQRLAVTVAMPLSCRS
                     TASEAAYFTRKDEVLHALTSHFASAPFEMDWQLNCLDRRGQGLDGVYLTLTGTSAEDA
                     DSGQVGRGNRANGLIAFSRPTGGEATAGKNPAAHAGKVYSVLSHRLARLIHDRLPALL
                     EVYVHLVVRIGEPVDRPWTGVQVRLPAGAELGDVEGEIQRVVEAELARLPAFRAELIR
                     GAHPVC"
     gene            3466..4533
                     /gene="rtcA"
                     /locus_tag="VFX87_02010"
     CDS             3466..4533
                     /gene="rtcA"
                     /locus_tag="VFX87_02010"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX4993707.1"
                     /translation="MTAPAIAIDASHGEGGGQVLRTALSLAVALGRPVTLRQIRARRP
                     RPGLQPQHLTVVRALGAISDALVTGDALDSTDVTFVPRTLRGGTYHFDIGAIRGSAGS
                     VALLFQALLLPLSLAAQPSRLRLRGGTHVPWSPPVPYLTAVFLPALGRIGVQAALTLD
                     RWGWYPAGGGEVDAVITPSRSWRALRWAHPTGPPILEGVSAVSRLPRSIAERQRARAL
                     DRLGAAGLSPTITLVEDRTALGPGTLTMLTAAGESARAGFSALGRRGVRAETVADEAV
                     DPLLAYLGSGATADDHLADQLIPFLALADGPSTFTCPALSTHLRTVAWVVEQMLPVHV
                     TLDEGRPARVEVRTAPGAPQG"
     gene            complement(4546..7017)
                     /locus_tag="VFX87_02015"
     CDS             complement(4546..7017)
                     /locus_tag="VFX87_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4993708.1"
                     /translation="MIRRLELGDLRAVCDPGTLPFRSTAELPPVDGLIGQERAATATA
                     FGVGMRHAGYNLFVLGPARTGKTSSMKRELARAAEREPVPPDYCYVHNFADPYRPTAL
                     EVPAGRGRGLREHMRRLAEECRMRLPRAFESEEFERQKAKILEDLGRRQQEEMSHLEQ
                     VARAHRFAVLRTPAGMGLAPAPRGEPLSPSDYAALPDAVKEEMQRESAAVEQQMDMSL
                     RRIRQVEREARDAHERLVREVAAAAVRPLFQEIREEFSGLPDVERYLTEVEQDLVAHA
                     EELRSTAEMKPALPFMPTPGRFLDRYEVNVLVDRHGLSGAPVVLEPNPTYGNLMGRTE
                     QRAHFGTLVTDFSLIRPGALHRANGGYLVLDAVDVLRHPLVWDALKKALKTRSIRIED
                     PLEEWRMTSSAGLAPQPIPLSVKVVLIGSPFLYYLLSALDEDFNELFKVKVDFDDSLP
                     RTSDFELLYARFVGSVCREEGLLAFSADGVARLIEHCSRLIADQSRLTSRLGDVLDLV
                     RESSFWAGQRGHGLVVADDVDHSIAQKTYRANLLEERTRRIITEGTLLIDTEGRAVGQ
                     VNGISVLALGEHVFGRPARITARTYAGEPGVVDIEREAKLGGPIHSKGVMILAGYVAG
                     RYARERPLALSASIAFEQQYEEIECDSAASAELYALLSSLTGIPLDQAIAVTGSVNQR
                     GEIQPIGGVNEKIEGFFDVCRARGLTGRQGVLIPQANVRHLMLRRDVVDAVRAGRFQV
                     YAITTVDEGLAVLTGCEAGERGADGRYPAGSVNAAVESALAANLERIKEMRVEKPRLI
                     VQPTEEPAGPWSPGQDGRPAGPKEE"
     gene            complement(7028..7873)
                     /locus_tag="VFX87_02020"
     CDS             complement(7028..7873)
                     /locus_tag="VFX87_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX4993709.1"
                     /translation="MRPAGARGPAEEGEDPRLSLALTLAGEAGRLAIAGRPGPDVRWK
                     GPGDRVTATDVQIQAHLTAAIARRFPQDGIVAEEAAVRVATDREFTWIVDPLDGTNNY
                     ALGIPCFTISLGLLRGGEPYAGVVHEPNTGFRCWTLRGHGAFAGDRRLALAPRALDAA
                     SNIAVRVPVAAELEPLVSRWLREHKFRGFGSVALHLAFAALGAIDLVLDHKAALWDLA
                     AGATVLLEAGGCITTPAGRPLFPFDVAGYRGAPVPFLAGNPLAHATAAASCRALVPGV
                     ERGEW"
     gene            complement(7870..8997)
                     /locus_tag="VFX87_02025"
     CDS             complement(7870..8997)
                     /locus_tag="VFX87_02025"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX4993710.1"
                     /translation="MDLHARLTALSKIDRAPAPVVTAYLNTRWADEHQRDRVRIFLKN
                     QLAEARRAPGPRPSKADLDWVEAQGEALLNQAMMPETSGVALFACEALGLREILPSRV
                     PFENAFTVAETPMLRPLLELGEQAPSTLVVFVDTETARLVPLTATGAGEEVTLAADVP
                     GHHSRGGWAQLAQGHYQRHTQDHRARHFEAVAQSLIGLMDSQGLRSIVLAGEPRNIAV
                     FRKELPPRLATLVVGTVAGARHEAIGLIVDRAAEYLPHVQGQREAEGVDAALTAAAKR
                     GKGSAGLEATLTAVNRGAVHRLYLLKGWSVPGRRCTRCGTLQGEFTWTCPSCGGEALA
                     VELGEAMAERVAAAGGTVETIEIHQPLAVAGGVAAELRYPL"
     gene            complement(9011..9397)
                     /locus_tag="VFX87_02030"
     CDS             complement(9011..9397)
                     /locus_tag="VFX87_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX4993711.1"
                     /translation="MEPIKITGLERDPLRGRATRLMSEALAKLAVAPVKGQASFFDDN
                     GPKGGRAMRCALTVRLPFRPSIRVERSAVTPRLAFDAAFAVLERQLERYRERDRDNKR
                     HPKKYFAARRAVAGEAPPPRKRTRRG"
     gene            complement(9449..12079)
                     /locus_tag="VFX87_02035"
     CDS             complement(9449..12079)
                     /locus_tag="VFX87_02035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX4993712.1"
                     /translation="MSDTSTAPAAEAGLTTLEARRRLRRIGPNRVADRGEAPLWRIAL
                     AQFGSLVVLLLLAASGIAWGLGERAEAVAILTALVLNAAIGFVSEWRARVSLARLRAL
                     AVPQAMVRRDGYVTRIAAAELVPGDVVILDAGAVVPADGRLLRAAALQVDESALTGES
                     AAVWKDAAEPSGADTPLAERANMLYLGTTILSGSGVALVTATGPATELGRIGRLVASA
                     GSRRTPLERQVEALGRRLIVLAVGICAVVGLAGILHGEPVGLMLETAVSLAVAAVPEG
                     LPAVVSLALAAGLWRLARRGALVRRLAAVETLGSTTVICADKTGTMTENQMTVTSLRF
                     GGRRVTVGGGGRSTAGSFADEAAGVVAPLADPHLTLLLTAGALCNDAGLRAAPDGLHL
                     HGDPTEAALLVAAVKAGLDPAELARAWPRRREIPFDPARRMMATFHVMPGAGAVLLVK
                     GAPGVILERATHRHTPAGPVALTEEDRARLLEDNRAMAGEGLRVLAFAWRRVEAIEDA
                     TVADLTFLGFAGLVDPVRSDVADAIAACREAGIRTIMLTGDQQLTALTVGRRLGLEPE
                     AIRSRVTPEGKLEMVAELQSRGEIVAMTGDGVNDAPALVRADIGVAMGRHGTDVARES
                     ADLVLTDDNFATIVEAVKEGRVIYANLRKVIRFLFSCNLSEILTIFAAILLGYPTPLL
                     PLQILWVNLVTDILPATALIRDPADDDVMGHPPRDPGEALVTWRSGARILAEGGLLAA
                     GVLSTFLWTVWREGPGAPAGTMAFTALVLIHPFQAMSCRSERLNWWQLRPNPWVPLSL
                     LALFALQWLAIEWGPLARLLGTRPLSGTNWLVLVLGVLWPVALLEALKAWGRFTTLRP
                     AYPTVVTQPPPESARTPRKD"
     gene            12109..12672
                     /locus_tag="VFX87_02040"
     CDS             12109..12672
                     /locus_tag="VFX87_02040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4993713.1"
                     /translation="MAEYPKELEREVTLRDGSRLHLRPIRPEDQDRLIAFYDRLSRHT
                     AYQRFFAVMKRLPPDWARLLANVDYERRLALIAEHGPPEAPELVGVARYEATDQADTA
                     EIAFVIQDGWQSRGLGTLMLDALLAAAEARGIRRFRAYVLAGNMRMIDLLVRFTDVQE
                     RVTESGVTELLLARRPASAPRQGGGTP"
     gene            complement(12651..13253)
                     /locus_tag="VFX87_02045"
     CDS             complement(12651..13253)
                     /locus_tag="VFX87_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4993714.1"
                     /translation="MTASGRSLANCLLAGLVLLLTGCVTAYQHGQTALHEGRYLEAAA
                     RFGEALADDPASPDALLGLGIAQYHLDSFHAAVGSLGRAVLAVPSHAEARLYLALTYL
                     ALEDQDSAARHLEAVAGLGVHARTAAQASRAAALLHLGPLSLEIREFVRKSLEDEADW
                     SRDVIEARLTPHMYLGPAWFGRDPAGWSPLGWYPYGVPPP"
     gene            complement(13250..13708)
                     /locus_tag="VFX87_02050"
     CDS             complement(13250..13708)
                     /locus_tag="VFX87_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4993715.1"
                     /translation="MQVKELMSRELVTIGPKESCLDAVVRMQRARVRHLPVVNRDGLL
                     VGIVTDRDLRHHLFTPRVFEVLGSTPVDVLLDGVRVAEIMATDVMTVEAGASVGDAAG
                     TMRKHRVGSLPVMENGRMVGIVTEVDILRQIVRADATTAPECAEIIVSYP"
     gene            complement(13727..13819)
                     /locus_tag="VFX87_02055"
     CDS             complement(13727..13819)
                     /locus_tag="VFX87_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX4993716.1"
                     /translation="MIEPVRDHWIIVLGVVILLGALLVWWRTRR"
     gene            complement(13816..14196)
                     /locus_tag="VFX87_02060"
     CDS             complement(13816..14196)
                     /locus_tag="VFX87_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993717.1"
                     /translation="MRRRVLVVDDEADFLATYERLLRRQGYDVVTATSRSAGLAALAG
                     EAPHLVISDIRLPDGDGLDVVRAACGGREPVPVIVVTGYPSDETRRAALAAGATTVLA
                     KPFTAAVLLEAIRSSVDGDRKRAP"
     gene            complement(14202..15314)
                     /locus_tag="VFX87_02065"
     CDS             complement(14202..15314)
                     /locus_tag="VFX87_02065"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX4993718.1"
                     /translation="MSWAIWITGPPGSGKSVIARGVEAELRALGQPVIRLELDEIRQS
                     VTPAPRYTDRERDIVYRALGYMAALLVETGTPVLIDATAHRRIWRDLVREAVSHFAEV
                     QLVCPLAICQERERERAPGHAPRNIYRQAGHPGATVPGVDVAYEAALAPEVLIDTSTH
                     TIEESVAQVVRLAGRLAGVTGGASAPAVGGEPGWAIWITGRPGSGKSTLAARVAEALR
                     ERGARVRLLDLGPVQRFLLGEQPGGEAEQDLVHRALAYTAKLLTDAGVSVIVDATAAR
                     RAWREAARALIPCFAEIQLLCPTEVCLERERAVRWGLTFEAAAPAPAPAKPDIVFDYE
                     EAARPDLAIRTDVYDAHAAAERVLSMIRQLAEGIRT"
     gene            complement(15311..16411)
                     /locus_tag="VFX87_02070"
     CDS             complement(15311..16411)
                     /locus_tag="VFX87_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4993719.1"
                     /translation="MEAALSAYLASVFAGPVEVVALRPLGGADAEGRDPKGFGYGVPF
                     EVECVVNGSSRNLVVARTRPVQGFGHDYPEDRAWQALYSHRAYNSFPAHVRSLDVGFI
                     RESGTLVSAGDATEFFQLVEKADGALYWRDLDRLLTEPLRPFDVARARALGAFLAHTH
                     ATRRDEPTLYERRIRELVAHGECLMGILDSYPHPYALLPPPVCEALEHDAVSWRWRLR
                     GRAHRLACVHGDFHPWNLLFRDGTDFSVLDRSRGEWGEPADDVSALGINYLFFGLRKA
                     ALEARRGLAEPFVTLFQAFLEAYTDVSKDPELFEVMPPFLAFRALVIGHPRWYPALAR
                     RTREALFGFARSLMAGEPLTAAGVPELLGGIA"
     gene            complement(16416..16862)
                     /locus_tag="VFX87_02075"
     CDS             complement(16416..16862)
                     /locus_tag="VFX87_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4993720.1"
                     /translation="MSRIRRILHPTDFSKASSAAYKRAVEMAKGNRAELLLVHVLTPA
                     IPMMADGYVSPQVYEDMANATRAYGQKHLAALVGKAKQAGVRVKGLLLEGVPHERIAQ
                     AARSRKADMVVIGTHGRTGFAKLFLGSVASRVLAVAPCPVLTVRGK"
     gene            complement(16859..17371)
                     /locus_tag="VFX87_02080"
     CDS             complement(16859..17371)
                     /locus_tag="VFX87_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4993721.1"
                     /translation="MKPNKILVPLDGSEVAEAAIAPALEMAQAGSPLLILMRAADPRI
                     LPGTDVIGTQVQAVREAEDYLAGVKAKLEKTGAGRIETHVWYGPAAPAVVEAAKLQKV
                     DLIVMSTHGRSGLGRIIFGSVAESVLRGTRVPILLVRPPRAPVEVPAGQGEARPAEKA
                     QPTSRTEVLR"
     gene            complement(17509..>17925)
                     /locus_tag="VFX87_02085"
     CDS             complement(17509..>17925)
                     /locus_tag="VFX87_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4993722.1"
                     /translation="FLKRYGQGRVVAMDDAVVEALERHAWPGNVRELQNVMERACALA
                     DGHMVTVRDLPDHVARAVSRPTAPSAGPASMAGQPAGIDLTLKAAKEQWLQVLEVSYL
                     RDLLARHDGNVSSAAKAAGIDRKTFHRLINKYDLRA"
BASE COUNT         2571 a         6581 c         6245 g         2528 t
ORIGIN      
        1 tcgcgaccga cacggcgttc tcgaggccaa ggcgcaccac cttggccggg tcgacgatgc
       61 cggcctcgta gaggtcgacg aatttcttga ccgcggcgtc gaagccgacg ttcgcggcgc
      121 tcgcgagcat cttgtcgacg accacgccgc cgtcggcggc cgagttctcg gcgatctggc
      181 gcgtcggcgc ctcgagcgcc cgcctcagga tctggagccc cgtgcgctcg tcgccctcgc
      241 actgtgcttc ttcgcgctcc acggcgtcga tggcgcgcag gagcgcgagc ccgccgcccg
      301 gcacgatgcc ctcggccacc gcggccttgg tggcgttgat ggcgtcgtcg agcgcttccc
      361 tcttgctctt catctccgat tcggaggggg ccccgacccg gacgaccgcc acgccgcccg
      421 agagtttggc cagccgctcc tggagcttct cccggtcgta gtcactcgtg gtcttgtcga
      481 tctgcgctcg gatctgctgc ttgcggccgt cgatccgcgc cttgtcgccg ccgcccccga
      541 tgatggtggt ggtgtccttg tccaccacga cgcgggtggc ccggccgaga tcctcgatgc
      601 tgagggcctc gagcttgatg ccgatctcct cggtcaggac acgaccgccg gtcaggatgc
      661 ccatgtcctc gagcatctcc ttgcgccggt cgccgaatcc cggcgccttc accgcgacgc
      721 acttgagaga gccgcggatc ttgttgacca cgagggtggc gagcgcctcc ccctcgacgt
      781 cctcggcgat gaccaggatc gagcgccccg cgcgcgccgc ctgctcgagg atcgggacca
      841 ggtccttcat cacgccgatc ttccggtcgc tgaggaggac cagggtgtcc tcgaggaccg
      901 cctccatctt ctccgggtcc gtgatgaagt acggggacag atatccgcgg tcgaactgca
      961 ggccctcgac cacctcgagc tgggtctccg tcgtcttgga ctcctcgacc gtgatcacgc
     1021 cttcgttgcc caccttctcc atcgcgtcgg cgaccatctc gccgatgctc tggtcgttgt
     1081 gggcggagat ggtggcgacc tgcgccttct cgagccggct cttcaccggc tgcgagagca
     1141 gcttcagcgc ctccaccgcc gcgcggacgc cgcgatcgag ccccttcttc agatccaccg
     1201 cgctcgcccc cgccaccacg ttgcgcacgc cgtcggcgaa gatcgcgtgg gcgaggatcg
     1261 tggacgtgct ggtgccgtcc cccaccgcgt cgccggtgcg ctccgcggcc tggcggatca
     1321 tctgggcccc caggttctcc acggggtcct tcagctccat ctccttggcg atcgtgacgc
     1381 cgtcgttgca gacaaccggc gcgccgaagc gcttctcgag gaggacgcac ttggacttcg
     1441 gccccagcgt gacgcgaacc gcgtcggcga gggcggcgac cccgcgcagg atgcgctcgc
     1501 gcgcttcgga acggaacaag acctgtttgt gtgccatgtc ctctatctcc ttcggctagg
     1561 ggcgcggccg cgcgccccgg ccgcgcggat ggggatgcac cgggaacgcc tgcgcttcgt
     1621 ggatcgcgaa atgacccgag cgggcctcgg ccagccgcag gtagtccgcc gtgcgcatcc
     1681 ggatcgcggt ccggtgatcg ccggccggga gcacgatgtc gcgctgctcg accagcgact
     1741 cgtccaccac ggtggggatg ccgaagagcc ccccgaacgg cggagtcgcg cccagcgcgc
     1801 agccgggaag acccgccagt atctcctcga cgctggcgag acggatctcg ccacggccga
     1861 tcagaccctt gagccggtcg aggtcggccg tgcaactggc cggcagcacg gccaaggcga
     1921 ggccgaccgg gtccttgacc acgacggtct tggcccagga ccagccggag acgtgagctt
     1981 tggccgcttc ttcctgggcg gtgaaggctt ccagatgcgg caccagctcg tagcggacgc
     2041 cccggacccc gaggagctct tccagtcgct cgtgcatgac gctcgatatg cttgcaagcg
     2101 acataccctc tcgcagggga ccgaaaagaa agccgattcg ggggccgcgc tcctctcgac
     2161 gggacagcac gcacgcctca ggtgggacag ccgtaccccg ccccggcggt cagcagacgg
     2221 ggtgcgcgcc gcggatcagc tccgcccgga acgcgggcag gcgggccaac tcggcctcca
     2281 cgacccgctg gatctccccc tccacgtctc cgagctcggc gccggccggc aggcgcacct
     2341 ggacgcctgt ccacggtcga tcgaccggct cgccgatccg gacgaccagg tgcacgtaaa
     2401 cctcgaggag cgccggcagg cggtcgtgga tgagccgcgc cagccggtga ctcaggacgc
     2461 tgtagacctt cccggcgtgc gcggcgggat tcttgcccgc ggtggcctcg cccccggtgg
     2521 gacgcgagaa ggcgatgagc ccgttggcgc ggttgccacg ccccacctgg cccgaatccg
     2581 catcttcggc cgaggtcccg gtcaacgtga gatacacgcc gtccaggccc tgcccgcgcc
     2641 gatcgagaca gttgagctgc cagtccatct cgaagggcgc cgaggcgaag tggctcgtga
     2701 gggcatgcag cacctcgtcc ttccgcgtga aatacgcggc ttcggaggcg gtggagcggc
     2761 acgacagggg catggccacc gtcacggcca gacgctggtc gtgccgcacg cccatcacct
     2821 tgacgtcctg gcccgtgtcc gggaaggcgg ccttgaatgc gcccgagttg aggtgtcgct
     2881 cgatcgcgag cacgagctcc tcggtggggc tcaggggagc gtagcccgat gccccggacg
     2941 tgtcattgga ggcgatctcg gtctcgcctt ccgtgtacac ccggcggagc tcctccgagc
     3001 cgggcgccaa cgccactcgc gtgaccagat ccttgccggc ccgcaccccg ggcagattgg
     3061 cgcggaccca ttcgtcgact gcgtgacgaa ccgtctcgac cacgggaagg ggccgtccat
     3121 cgaggacgtc ggtggcgcgg tcgccgatga tcagctccat cgggcgcgtc acctcgccgc
     3181 cgccgaagcg cttgacacac tggcccgcca ccagcagcgc cttgtcgatg ttgtagtgcg
     3241 cgatgacgcc ggcacgctcg aggtacagac ggccgagcgc gacggaggcc gcctccacca
     3301 ccgagtcgca gatggtgtcc gggtgtccga gtcccttccg ctccaccagc tcgatccgcc
     3361 ggcgcgggac cggcgtgccg ccaatggatt cgatcacgag gctcatcgaa ggcctgcgga
     3421 cccgagcggg cagcgccgcc gcgccgggtc gggtactgta cgggcgtgac cgcgcccgca
     3481 atcgcgatcg acgcctctca cggcgagggc ggcggacagg tgctccgcac cgcgctctcg
     3541 ctggcggtcg ccctcggccg gccggtgacg ctccggcaga tccgcgcccg gcgcccccgg
     3601 cccggactgc agccgcagca tctcaccgtg gtccgcgccc tcggcgcgat cagcgacgcc
     3661 ctggtcaccg gcgacgcgct cgactccacc gacgtgacgt tcgtgccgcg gacgctgcgc
     3721 ggcggcacct accacttcga tatcggcgcc attcgaggca gcgccggctc cgtggcgctg
     3781 ctcttccagg cgctgctcct gcccctcagc ctcgcggcgc agccgtcccg cctccggctc
     3841 cgcggcggca cccacgtgcc ctggagccct cccgtgccct acctcaccgc cgtcttcctg
     3901 cccgcgcttg gccggatcgg cgtccaggcc gccctcacgc tcgaccgctg gggctggtat
     3961 ccggccggcg ggggggaggt tgacgccgtg atcacgccca gccgctcctg gcgggctctt
     4021 cggtgggcgc acccgaccgg gccgccgatc ctcgagggcg tctccgcggt gtcgcgcctg
     4081 ccgcgctcga tcgccgagcg gcagcgcgcc cgggcgctcg atcggctcgg ggccgccggg
     4141 ctgtcgccca caatcacgct cgtcgaggac cggaccgccc tcgggcccgg gacgctgacg
     4201 atgctgaccg ctgccggcga gtcggcgcgg gccggcttct ccgccctcgg ccggcgcggg
     4261 gtccgggccg agaccgtggc ggacgaagcg gtggatccgc tcctcgccta cctggggagc
     4321 ggcgcgaccg cggacgatca cctggccgac cagctcatcc cgttcctcgc actggccgac
     4381 ggtccctcga cgttcacctg tcccgccctg tcgacacacc tgcgcacagt cgcctgggtg
     4441 gtcgagcaga tgcttcccgt ccacgtgacc ctcgacgagg gtcgcccggc tcgggtcgag
     4501 gtccgcaccg cgccaggggc gcctcaagga tgaccggtcc atcggtcatt cctccttggg
     4561 cccggccgga cgaccgtcct ggccgggcga ccagggcccc gccggctcct cggtcggctg
     4621 cacgatcagg cgcggcttct ccacccgcat ctccttgatt cgctccaggt tggcggcgag
     4681 ggcgctctcc acggcggcat tgacgctgcc ggccgggtac cggccgtcgg ctccccgctc
     4741 gccggcctcg cagcccgtca gcaccgccag cccttcgtcc accgtggtga tcgcgtagac
     4801 ctggaagcga cccgcgcgca ctgcgtccac cacgtcccgg cggagcatca ggtgccggac
     4861 attggcctgc gggatcagca ctccctggcg accggtgagc ccgcgggctc gacagacgtc
     4921 gaagaagccc tcgatcttct cgttcacgcc gccgatcggc tggatctcgc cgcgctggtt
     4981 caccgagccg gtgaccgcga tggcctgatc caggggaatg ccggtgagac tcgagagcag
     5041 cgcgtacagc tcggcggacg cggcgctgtc gcactcgatc tcctcgtact gctgctcgaa
     5101 cgcgatggac gccgagagcg cgagcgggcg ctcccgcgca taacgccccg ccacgtagcc
     5161 ggccaggatc atcacaccct tggagtggat cggcccgccc agcttggcct cccgctcgat
     5221 gtccaccacc cccggctcgc cggcgtaggt gcgcgcggtg attcgcgcgg ggcgcccgaa
     5281 gacgtgctcg cccagcgcca gcaccgagat gccgttgacc tgccccaccg cgcgcccctc
     5341 ggtgtcgatc agcagggtgc cttcggtgat gatccggcgc gtgcgctcct ccagaaggtt
     5401 cgcgcggtac gtcttctggg cgatcgagtg atcgacgtcg tcggccacga cgagcccatg
     5461 gccgcgctgg ccggcccaga acgaggactc gcggaccaga tccagcacgt cgcccaggcg
     5521 cgaggtgagg cggctctgat cggcgatgag ccggctgcag tgctcgatga gccgggcgac
     5581 tccgtccgcc gagaacgcga ggagcccttc ctcgcggcag acgctgccca cgaaccgagc
     5641 gtagagcagc tcgaagtccg atgtacgcgg cagcgagtcg tcgaaatcca ccttgacctt
     5701 gaacagctcg ttgaaatcct cgtcgagggc cgacaacagg tagtagagga acgggctgcc
     5761 gatcaggacc accttgaccg agagcgggat cggctggggc gcgagtcccg cgctgctggt
     5821 catccgccat tcctccagcg ggtcctcgat ccggatcgat cgggtcttga gcgccttctt
     5881 gagcgcatcc cagaccagcg ggtggcgcag gacatccacg gcgtccagga ccaggtagcc
     5941 cccgttcgcc cggtgcagcg cccccggccg gatgaggctg aagtccgtga ccagggtgcc
     6001 gaagtgcgcc cgctgctcgg tgcgccccat caggttgcca taggtgggat tcggctcgag
     6061 cacgaccggc gcgccggaga gcccgtggcg gtccaccagg acattgacct cgtagcggtc
     6121 caggaagcgc ccgggagtcg gcatgaaggg cagcgccggc ttcatctcgg cggtgctgcg
     6181 gagctcctcg gcgtgggcca ccaggtcctg ctccacctcc gtcagatatc gctcgacgtc
     6241 gggcagcccg gagaattcct cccggatctc ctggaacagc ggacggaccg cggccgccgc
     6301 cacctcacgg accagccgtt cgtgggcgtc ccgcgcctcg cgctcgacct gccggatgcg
     6361 gcgcagggac atgtccatct gctgctcgac ggccgcgctc tcacgctgca tctcctcttt
     6421 caccgcatcg ggaagggccg cgtagtccga cgggctcaac ggctcgccgc ggggcgcggg
     6481 ggcgagaccc atcccggccg gcgtgcggag gactgcgaac cggtgggccc gggcgacttg
     6541 ctccaggtgg ctcatctcct cctgctggcg ccgacccagg tcctccagga tcttggcctt
     6601 ctgccgctcg aactcctcgc tctcgaatgc ccggggcaga cgcatccggc actcctcggc
     6661 cagccgacgc atgtgctccc ggagcccgcg cccgcgcccg gcgggcacct cgagcgcggt
     6721 gggccggtac ggatcggcga agttgtgcac gtagcagtag tcgggcggca ccggctcgcg
     6781 ctcggcggcc cgggccagct cgcgcttcat cgacgaggtc ttgccggtac gggccgggcc
     6841 gaggacgaag aggttgtagc cggcgtggcg catgcccacg ccgaaggcgg tggcggtcgc
     6901 cgcgcgctcc tgtccgatca acccgtccac cggcggcagc tcggcggtgg atcgaaacgg
     6961 cagcgtgccc ggatcgcaca ccgcgcgcag atcgccaagc tcgaggcgac gtatcatgat
     7021 cggaggccta ccactcgccc cgctcgacgc cggggacgag cgcccggcag gacgcggccg
     7081 ccgtggcgtg ggcgaggggg ttaccggcca ggaatgggac cggcgcgcct cggtatcccg
     7141 ccacgtcgaa gggaaagagc gggcgacccg ccggggtcgt gatgcaaccg ccggcctcga
     7201 ggagcaccgt cgcgccggcg gccaggtccc agagggcggc cttgtggtcc agcaccagat
     7261 cgatggcgcc gagcgccgcg aaggccagat ggagggcgac cgagccgaat ccgcggaatt
     7321 tgtgctcgcg cagccaccgg gagaccagcg gctcgagctc ggccgcgacc ggaacgcgca
     7381 ctgcgatgtt cgacgcggca tcgagggcgc gcggggccag agccaggcgc cggtcgcccg
     7441 cgaaggctcc gtgccctcgc agcgtccagc agcggaaccc ggtgttgggc tcgtggacga
     7501 cccccgcgta cggttccccg ccgcggagga gcccgagcga gatcgtgaag cacgggatgc
     7561 ccagcgcata gttgttggtt ccgtccaggg ggtcgacgat ccaggtgaac tcgcgatctg
     7621 tggccacccg gaccgcggct tcctcggcca cgatgccgtc ctgggggaag cgccgggcga
     7681 tggcggcggt gaggtgggcc tggatctgga cgtccgttgc ggtgacccga tcgccggggc
     7741 ccttccaccg cacatccggg cccggccgcc cggcgatcgc cagccgtccc gcctcgcctg
     7801 cgagcgtcag cgccagcgag aggcgcggat cctccccttc ttcagcggga ccccgcgccc
     7861 ccgccggcct cacaggggat agcgcagctc ggccgcgaca ccgccggcca cggccagagg
     7921 ctggtgaatc tcgatggtct ccacggtgcc gccggcggcg gcaacgcgct cggccatcgc
     7981 ctcgcccagc tccaccgcca gcgcctcacc gccgcagctc gggcaggtcc aggtgaactc
     8041 gccctggagg gtcccgcacc gggtgcagcg gcggccgggc acgctccagc ccttgaggag
     8101 gtagaggcgg tggacggcgc cccggttgac ggcggtgagg gtggcctcga ggcccgcgct
     8161 ccccttccct cgcttggccg ccgcagtcaa cgcggcatcg acgccctcgg cctcccgctg
     8221 cccctgcacg tgcgggagat actcggcggc tcgatcgacg atgagcccga tggcctcgtg
     8281 ccgggcgccg gccacggtcc cgacgaccaa cgtggcgagg cgcggcggca gctccttgcg
     8341 gaacacggcg atgttgcggg gctcgcccgc cagcacgatc gaccgcaagc cttgactgtc
     8401 catcagcccg atcaggctct gggccaccgc ctcgaagtgc cgggcccggt ggtcctgcgt
     8461 gtgccgctgg tagtgccctt gcgcgagctg ggcccagccg ccccggctgt gatggccggg
     8521 cacgtcggcg gcgagcgtga cctcctcgcc tgctcccgtc gcggtgagcg ggacgagccg
     8581 cgcggtttcc gtgtcgacga acaccaccag cgtcgacggc gcctgctccc cgagctccag
     8641 cagcgggcgc agcatcggag tctccgccac cgtgaaggcg ttctcgaacg gaacgcggct
     8701 gggaaggatc tcgcggagcc ccagcgcctc gcacgcgaag agggcgactc cgctggtctc
     8761 gggcatcatc gcctggttca ggagcgcctc gccctgggct tccacccagt ccagatcggc
     8821 cttggacggg cgcgggccgg gcgcgcgccg ggcttctgcc agctgattct tgaggaagat
     8881 ccggacgcgg tcgcgctggt gctcatccgc ccaccgggta ttcaggtagg ccgtcaccac
     8941 cggggccggg gcgcggtcaa tcttgctgag cgcggtcagt cgggcatgca agtccatgga
     9001 cgggcctccc tcatccgcgc cgcgtccgct ttcgcggcgg cggcgcctcc ccggcgaccg
     9061 cccgacgcgc cgcgaagtac ttcttggggt gccgcttgtt gtcgcgatcc cgctcgcggt
     9121 accgctcgag ctggcgctcg agcacggcaa aggcggcatc gaaggcgagg cggggcgtca
     9181 cggcgctccg ctccacgcgg atgctgggcc ggaacggcag ccgcaccgtc agggcacatc
     9241 gcatggcccg gccgcccttg ggcccgttgt cgtcgaagaa cgaggcctgt cccttgaccg
     9301 gcgcgaccgc cagcttcgcg agggcctcgc tcatgagccg cgtggcccgg ccacgcagcg
     9361 gatcccgctc cagcccggtg atcttgatgg gttccacgtt agtagtcata tcggcttcag
     9421 agagcaacgc gggtgccaac gcgcatgctc aatcctttcg cggtgttctc gcggattccg
     9481 gcggcggctg ggtcaccacg gtggggtagg cgggcctcaa tgtggtgaag cggccccagg
     9541 ccttgagggc ctccagcaga gccaccggcc acagcacccc cagcaccagg acgagccagt
     9601 tggtgccgct cagcggccgg gtgccgagca gccgcgccag gggtccccac tcgatcgcga
     9661 gccactgcag cgcgaagagg gccagcagcg acagcgggac ccacggattg ggccggagct
     9721 gccaccagtt gagccgctcc gagcggcagc tcatggcctg gaacgggtgg atgaggacga
     9781 gcgccgtgaa cgccatggtg ccggccggcg cgcccgggcc ctcacgccac accgtccaga
     9841 gaaaggtgct gagcaccccg gccgccagca gcccgccctc ggccaggatg cgcgcacccg
     9901 atcgccaggt gaccagcgcc tcgccaggat cgcgcggggg gtgccccatg acgtcgtcgt
     9961 cggccggatc ccggatcagg gcggtcgcgg gcaggatgtc ggtcaccagg ttcacccaga
    10021 ggatctggag cggcaggagc ggcgtgggat agcccagcag gatggccgcg aagatggtga
    10081 ggatctcaga gaggttgcac gagaagagaa agcggatgac cttccggaga ttggcgtaga
    10141 tcacgcggcc ttccttcacg gcctccacga tggtcgcgaa gttgtcgtcg gtgagcacca
    10201 gatcggcgga ctcgcgcgcg acatcggtgc cgtgtcggcc catcgccacg ccgatgtcgg
    10261 cacggacgag cgcgggagcg tcgttgacac cgtcgccggt catcgcgacg atctcaccgc
    10321 ggctctgcag ctcggccacc atctcgagct tgccctcggg agtgacccgg ctccggatcg
    10381 cctccggctc gaggccgagc cgacggccga ccgtcagggc ggtgagctgc tggtcgccgg
    10441 tgagcatgat cgtgcggatg ccggcctcac gacaggccgc gatcgcgtcc gccacatcgg
    10501 agcgcaccgg atccacgagc ccggcgaatc cgaggaacgt gagatcggcg acggtcgcgt
    10561 cctcgatcgc ctcgacccgg cgccaggcga aggcgagcac gcgcaggccc tcgccggcca
    10621 tggcccggtt gtcctcgagc agacgcgcgc gatcctcctc ggtcagcgcg acgggaccgg
    10681 cgggcgtgtg acggtgcgtc gcgcgctcca ggatcacgcc gggggcgccc ttgaccagga
    10741 gcacggcccc ggcccccggc atgacgtgga aggttgccat catgcgacgc gccggatcga
    10801 aggggatctc ccgccggcgg ggccaggcgc gcgccagctc cgccgggtcg agcccggcct
    10861 tgaccgccgc caccagcagg gccgcctccg tcgggtcccc gtgcaggtgg agcccatcgg
    10921 gcgccgccct caggccggcg tcgttgcaca gggcccctgc cgtcagcagc agcgtgagat
    10981 gcgggtcggc gagcggggcc acgacgccgg ccgcctcgtc cgcgaagctg cccgcggtcg
    11041 agcgcccgcc accaccgacc gtgacccgac ggccgccgaa tcggagcgac gtcaccgtca
    11101 tctggttctc ggtcatggtg ccggtcttgt ccgcgcagat cacggtggtc gagcccagcg
    11161 tctccaccgc ggccagccgc cggacgagcg cgccgcgccg ggcgagtcgc cagagcccgg
    11221 cggcgagcgc gagcgacacc accgccggca ggccctcggg cacggcggcg acggcgaggc
    11281 tgaccgccgt ttccagcatc agaccgaccg gctccccgtg caggatgccg gccaggccca
    11341 ccaccgcaca gatgccgacc gccagcacga tcagtcgccg gcccagcgcc tccacctggc
    11401 gctcgagcgg cgtcctccgc gagccggcgg aggccaccag ccggccgatc cgcccgagct
    11461 ccgtcgcggg cccggtggcg gtgacgagcg ccacgccgct gccggacagg atcgtggtgc
    11521 cgaggtagag catgttcgcg cgctcggcca gcggcgtatc cgcacccgac ggctccgcgg
    11581 cgtccttcca gacggcggcg ctctcgccgg tcagggcgga ttcgtcgacc tgcagggccg
    11641 ccgcgcgcag cagccgcccg tccgcgggca ccaccgcccc cgcgtccagg atgaccacgt
    11701 cgcccggcac gagctccgcc gcggcgatcc gggtcacgta tccgtcacgc cggaccattg
    11761 cctgcggcac cgcgagcgcg cgcagcctgg ccagtgacac ccgggcgcgc cactcggaga
    11821 cgaagccgat ggctgcgttc agcacgagcg cggtgaggat cgccaccgcc tcggcccgct
    11881 cgccgagccc ccacgcgatg cccgaggcgg cgagcagcag caagaccacg aggctcccga
    11941 actgggcgag ggcgatccgc cagagcggcg cctcaccccg atcggccacg cgattgggcc
    12001 cgatccggcg aagacggcgc cgggcctcga gcgtggtgag ccccgcctcg gcggcgggcg
    12061 ccgtggacgt gtcgctcacc cgcgcgaccc gctacaatgg tcgcgccgat ggcggagtac
    12121 ccgaaggagc tcgagcgcga ggtcactctg cgggacggat cgcgcctgca cctgcgtccg
    12181 atccgccccg aggaccagga ccggctgatc gcgttctacg atcgcctgag ccgccatacc
    12241 gcctaccagc ggttcttcgc ggtgatgaag cgcctgccac ccgactgggc gcgtctgctc
    12301 gccaacgtgg actacgagcg ccggctcgcc ctgatcgccg agcacggccc gccggaggct
    12361 cccgagctgg tcggggtggc ccgctacgag gccaccgatc aggcggacac cgcggagatc
    12421 gccttcgtga tccaggacgg ctggcagagc cgcggcctcg gcaccctcat gctcgacgcg
    12481 ctgctcgccg ccgccgaggc gcgcggcatc cggcgcttcc gggcctacgt gctggccggc
    12541 aacatgcgca tgatcgacct gctcgtgcgc ttcacggacg tgcaggagcg cgtcacggag
    12601 agcggcgtca ccgagctgct gctcgcccgg cgccccgcct cggccccgcg tcagggtggc
    12661 ggaacgccgt agggatacca tccgaggggg ctccagccgg ccggatcgcg cccgaaccag
    12721 gccggcccca ggtacatgtg cggggtgagc cgcgcctcga tcacgtcgcg gctccagtcg
    12781 gcctcgtcct cgagactctt gcgaacgaac tcgcggatct cgagcgacag cggcccgagg
    12841 tgcagcaggg cggccgcccg ggaggcctga gcggcggtcc gcgcgtgcac gccgaggccg
    12901 gccaccgctt cgaggtgccg cgcggccgag tcctggtcct cgagagccag atacgtcaac
    12961 gcgaggtaga ggcgggcctc ggcgtgggac ggtacggcga gcaccgcccg tccgagcgag
    13021 cccaccgccg catggaacga gtccagatgg tactgagcga tgcccagtcc aagcagggca
    13081 tcgggcgaag ccggatcatc cgcgagagct tcgccgaacc gggcggcggc ctcgaggtag
    13141 cgaccctcgt gcagcgcggt ctgcccgtgc tggtaggccg tcacgcaccc ggtgagcagc
    13201 agtacaagtc ccgcgaggag acaattggcc aagctccggc cggacgcggt catggatagg
    13261 agacgatgat ctccgcgcac tccggggcgg tcgtggcgtc ggcccggacg atctggcgaa
    13321 gaatgtccac ttcggtgacg atgccgacca tccgcccgtt ctccatcacc gggagggacc
    13381 cgacgcggtg cttgcgcatc gttcccgccg cgtcgccgac gctcgcgccc gcctcgaccg
    13441 tcatcacgtc ggtcgccatg atctcggcca cgcgtacccc gtcgagcagt acgtccaccg
    13501 gcgtggagcc cagtacctcg aacacccggg gggtgaagag gtggtgacgg aggtcgcgat
    13561 ccgtgacgat tccgaccagg aggccgtcac gattcaccac cggaaggtgc cggacccgcg
    13621 cccgctgcat ccgcacgacc gcgtccaggc acgactcctt cggcccgatc gtcaccagct
    13681 cgcggctcat cagctccttg acctgcatgg cggcctcctt gccggctcag cgccgggtcc
    13741 tccaccagac gagcagcgcg cccagcagga tgacgacacc cagcacgatg atccagtggt
    13801 cacggacggg ttcgatcatg gcgccctctt ccgatcccca tccacgctcg agcggatcgc
    13861 ttccagcagc accgcagcgg tgaagggctt ggcgagaacc gtggtggccc cggccgcgag
    13921 agcggcccgc cgggtctcgt cggacgggta gccggtgacc acgatgaccg gcaccggctc
    13981 gcgcccgccg caggcggccc gcaccacgtc gaggccgtcg ccatccggca accggatatc
    14041 ggagatgacg agatggggcg cttcgccggc cagcgccgcc aggcccgccg aacgggacgt
    14101 ggccgtgacc acgtcatagc cctggcgccg gagcagccgc tcgtacgtgg cgagaaagtc
    14161 ggcctcatcg tcgaccacca gcacgcgtct acgcatggga ttcacgtccg gatcccctcg
    14221 gccagttgcc ggatcatcga gagcacccgc tccgcggcgg cgtgggcatc gtacacgtcg
    14281 gtcctgatcg ccagatcggg ccgcgccgcc tcttcatagt cgaaaacaat gtcgggcttc
    14341 gcgggcgccg gagccggcgc cgccgcctcg aacgtcaggc cccagcggac ggcgcgctct
    14401 cgctcaaggc acacctcggt cggacacagg agctggatct ccgcgaagca ggggatcagg
    14461 gcgcgggccg cctcgcgcca ggcgcgccgc gcggccgtcg cgtccacgat gaccgacacg
    14521 ccggcatcgg tcagcagctt ggcggtgtag gcgagcgcgc ggtggacgag atcctgctcg
    14581 gcctcgccgc ccggctgctc gcccagcagg aaccgctgga ccgggcccag gtccagcagg
    14641 cggacgcggg cgccccgctc gcggagcgct tccgccaccc gcgcggcgag cgtgctcttg
    14701 ccgctccccg gccggccggt gatccagatc gcccagcccg gctctccccc gaccgccgga
    14761 gcggatgctc ccccggtgac gccggcgaga cggccggcca gtcgcacgac ctgagccacg
    14821 gactcctcga tcgtgtgcgt gctggtgtcg atcaggactt cgggagccag agccgcttca
    14881 taggccacgt ccacccccgg cacggtcgcg ccggggtgtc cggcctgccg atagatgttc
    14941 cggggcgcgt gccccggtgc ccgctctcgc tcacgctcct ggcagatcgc gagggggcag
    15001 acgagctgca cctccgcgaa gtgagaaacg gcttctcgca cgaggtcacg ccagatgcgc
    15061 cggtgggcgg tcgcgtcgat gaggaccggc gtgccggtct cgacgagcag cgcggccatg
    15121 tagcccaggg cccggtacac gatgtcgcgc tcgcggtccg tgtagcgggg agccggggtg
    15181 acgctctgcc ggatctcgtc gagctccagc cggatgaccg gctgcccgag ggcgcgaagc
    15241 tccgcctcca ccccccgcgc gatcaccgac ttcccgctgc ctggaggacc cgtgatccag
    15301 atggcccagc tcatgcgatg cccccgagca gctccggcac gccggcggcg gtgagcggtt
    15361 cgcccgccat cagggagcgc gcgaatccga agagggcctc gcgggtcctc cgggccagcg
    15421 cgggatacca gcgggggtgt ccgatgacca gcgcgcggaa ggcgagaaac ggcggcatca
    15481 cctcgaagag ctccgggtcc ttcgacacgt cggtataggc ctcgaggaac gcctggaaga
    15541 gcgtgacgaa cggctcggcg aggccccgcc gggcctcgag cgcggccttc cggagcccga
    15601 agaagaggta gttgataccg agcgcgctca cgtcgtcggc cggctctccc cactccccgc
    15661 ggctccggtc cagcaccgag aagtcggtcc cgtcccggaa caggaggttc cacgggtgga
    15721 aatcgccgtg cacgcaggcc agccggtggg cgcggccgcg gagacgccac cgccacgaca
    15781 cggcgtcgtg ctcgagcgct tcgcagacgg gcggcggcag cagcgcgtag ggatgtggat
    15841 agctgtcgag gatccccatc aggcactcgc cgtgcgccac cagctcgcgg atgcggcgct
    15901 cgtagagcgt gggctcgtcg cgccgggtcg cgtgggtgtg cgcgaggaag gcgccgagcg
    15961 cccgcgcccg ggcgacgtcg aagggccgca ggggctcggt gaggagccga tcgagatcgc
    16021 gccagtagag cgccccgtcc gccttttcca cgagctggaa gaactcggtc gcgtcgcccg
    16081 ccgagaccag cgtccccgac tctctgatga agcccacgtc caggctacgg acgtgagccg
    16141 ggaaactgtt gtaggcacgg tggctgtaga gggcctgcca cgcccggtcc tccgggtagt
    16201 cgtggccgaa gccctggacg ggccgggtgc gtgctaccac caggttccgg ctggagccgt
    16261 tcaccacaca ctccacctcg aagggcaccc cgtagccgaa gcccttcgga tcccgcccct
    16321 ccgcgtcggc cccgcccaga ggacggaggg ccacgacctc gaccggcccg gcgaagaccg
    16381 aggccagata cgcggagagc gcagcttcca cgcggttact ttccccggac cgtcaggacc
    16441 ggacagggcg ccacggccag gacgcggctc gcgacgctcc cgaggaagag cttggcgaag
    16501 ccggtgcgcc cgtgcgtgcc gatgacgacc atgtcggcct ttctggagcg ggcggcctgc
    16561 gcgatgcgct cgtgcggcac gccttcgagc agcaggccct tgacgcggac acccgcctgc
    16621 ttggccttcc cgaccagcgc ggcgagatgc ttctgtccgt aggcgcgcgt ggcgttcgcc
    16681 atgtcctcgt agacctgggg cgagacgtag ccgtcggcca tcatggggat ggcgggggtc
    16741 agcacgtgga ccagtaacaa ctcggcgcgg ttgcccttgg ccatctccac cgcgcgcttg
    16801 tacgcggccg acgacgcctt ggaaaagtcc gtggggtgca ggatgcgtcg gatccggctc
    16861 atcggagaac ctccgttcgg cttgtgggtt gagctttctc ggccggccgc gcttcaccct
    16921 gaccggcggg gacctccacg ggcgcgcgcg ggggccggac caggaggatc ggcacgcggg
    16981 taccgcgcag gacggattcc gcgacgctcc cgaagatgat ccgccccagc ccgctgcgcc
    17041 cgtgcgtgct catcacgatc agatcgacct tctggagctt ggccgcctcg acaacggccg
    17101 gagccgccgg gccgtaccag acgtgcgtct cgattctccc cgcgcccgtc ttctcgagct
    17161 tcgccttcac cccggccagg tagtcctccg cttcccgcac cgcctggacc tgggtgccga
    17221 tcacatcggt ccccggcagg atccgcgggt cggcggctcg catcagaatc aggagcggag
    17281 agccagcctg tgccatctcc agggccgggg cgatcgccgc ttcggccacc tccgagccat
    17341 cgagtggaac caggatcttg ttgggcttca tgatgtcctc cctaggccac gggggatagc
    17401 aacggaagtg ccagcggaga aagacaagga aaccggaggg ttggagacgt cgagcgcgcc
    17461 ggacatgagg cggcgcctcc ccgctgggac aacgtcgccc cggtgcggct acgctctcag
    17521 gtcgtacttg ttgatgagcc ggtggaaggt cttgcggtcg atgccggcgg ccttggcggc
    17581 ggacgagacg ttgccgtcgt gacgcgccag caggtcccgg agataggaga cttccagcac
    17641 ctggagccac tgctccttgg ccgccttgag cgtgagatcg atcccggccg gttggccggc
    17701 catcgaggcg ggaccggcgg acggcgcggt aggccgcgag accgcccgcg cgacgtgatc
    17761 gggcaggtcc cgcaccgtga ccatgtggcc atccgcgagc gcgcaggccc gctccatcac
    17821 gttctggagc tcgcgcacgt tgccgggcca ggcgtgccgc tccagagcct ccacgaccgc
    17881 gtcgtccatc gcgacgaccc gcccctgccc gtagcgcttg aggaa
//
LOCUS       DASUFA010000055        13450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_814705, whole genome shotgun sequence.
ACCESSION   DASUFA010000055 DASUFA010000000
VERSION     DASUFA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814705"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1433)
                     /locus_tag="VFX87_02090"
     CDS             complement(30..1433)
                     /locus_tag="VFX87_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4993723.1"
                     /translation="MTDWTLEGLATAIRAGKISPVEATRECLARIERLDGGLRAFITV
                     DHAGALETARRLEAEQGRGVWRGPLHGVPLAFKDLCFIRGLPTSCGTSTAEYFQAEQD
                     CTVVARLAAAGAVTLGKLNMTELAMGPFGDNAHHGHAQNPWRAGHCTGGSSSGSAVAV
                     AGRLALGAVGSDTGGSIRLPAAACGIVGLKPTYGRVSRAGAMPLSWSNDHVGPMARTV
                     RDCALLLQVMAGRDALDVTSSPEPVPDYVSALGRTVAGLRVGVVENYFFQGIDAEMEA
                     AVRRAVDVIATLGARVSELRVPDPQTMSDVTNIVSRSEASTIHAQLLRERPHEIQPVV
                     RSRLELGMQVPAYDYLQALRLRARLTRAFIAQVFAEVDIVVAPVIPEAAPSLAHATVG
                     PVHELVARQGRFSRLTRPFNGLGLPALSVPCGFASAGLPLAYQIVGRPFDEPTVLRLG
                     DAYQQATDWHTRRPALS"
     gene            complement(1430..1582)
                     /locus_tag="VFX87_02095"
     CDS             complement(1430..1582)
                     /locus_tag="VFX87_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993724.1"
                     /translation="MDESRVQQAEAAALLAVIERMAADLALAEEPARFIAALEAGRPP
                     AGGDPE"
     gene            complement(1600..2949)
                     /locus_tag="VFX87_02100"
     CDS             complement(1600..2949)
                     /locus_tag="VFX87_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX4993725.1"
                     /translation="MTDYLDRLAAFVEGVAAERLTDDALRAARLVLLDTIGAMVAGSA
                     QPENRRLAEAMHERRSRATATLLGHGGKADPLLATFVNATAGVALEVDEGSRLGGGHP
                     AIHVVPGALAVAEDTGADGRRLLESIIAGYEVCSRLGGATTPKPNVHSHGTWGTIGTA
                     VAAARLAGLDAAGVRSVINLSASMSPANTWTTALEGATIRNAYPGRSGLEGLLAVDLH
                     RAGFTGLADAPRDVYGTILADRFEPERALDGLGGPLRIEQNYFKLYACCRYNHFALDA
                     LLAMTRAARLEAAQVERVVVTTIPFGLRMADPAPASMLGAKFSIPYAVAAALVLGRAD
                     VAAFVEPALSDPRIRSLAARVEVRVDPRMSPRRVDYPTALVEVRLRDGRTLSASTTVV
                     RGDFADPVSAGEVVGKFIPLVTDVLGADRARAVAELVDQVETLKDVRDLTALLAPAS"
     gene            complement(2960..4393)
                     /locus_tag="VFX87_02105"
     CDS             complement(2960..4393)
                     /locus_tag="VFX87_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4993726.1"
                     /translation="MSARAKSSSIDLIVRGGRVVTPTEVLETAVAVKDGQIVALGAEA
                     MLPPAERTIDATGKYVLPGLIDCHVHLGAVYDDWHTGPVAAAHAGLTTLLSFVEYDDQ
                     ARETLPQALKRLRDEAGQQSVVDFGFHFILNNQPYILDGLAEAFEQGVTSYKIFMTYK
                     RRPNRMCSDEFIAQVMERIAALGGVCQLHCENGDILYHLENKSIAAGRVKPTDFPATC
                     PPWTEEEAINRAILIGEMTQCPVYVVHLSTKGGLERIKEAQARGQRVWTETCPQYMLL
                     TEKEMEAWGPLAKIGPPLRPVGGGDGDALWGGSREGYIATVASDHSPRRKELKEPGWK
                     NIFVDGKGNPVPFGSPSIETLVPLMWSEGVVKRGLPVTWLARVMAENPARIFGLYPRK
                     GAIRVGADADLTIIDPDSDGTIKVADHHGIAGWTLYEGWKSRGRPWMTVLRGQVLLNQ
                     GRLEQKPGYGRYLARTGPVAPIAGGAG"
     gene            complement(4390..5268)
                     /locus_tag="VFX87_02110"
     CDS             complement(4390..5268)
                     /locus_tag="VFX87_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX4993727.1"
                     /translation="MARHIKVAAAQMGPNNEGTSREEIVERMLGLLDQAIVEGVEILA
                     YPEMALTTYFPKRVRDDFDQFFETEMPPKALAPLLQRAREARIACHVGFCEKDQGRYF
                     NTALLTDETGKLAGTFRKIHLPGAKQPDGHAQVYEPHFFESGNSGYRVFDAARAKVGV
                     AICQDRRFPESYRSLGLLGAEIVLIGYNTPLSPLALDLNELCLRAGAYQNNLFVVGIA
                     KAGVEDGMELIGGSCIVNPMGQVVAKAATTGDELVAARIDLDQMVPARKRWDFLGRRH
                     PEHYGLLTQPTRSGAR"
     gene            complement(5298..6089)
                     /locus_tag="VFX87_02115"
     CDS             complement(5298..6089)
                     /locus_tag="VFX87_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX4993728.1"
                     /translation="MSRHGHSGRPAGPGAPEPTFAEQARTLVHLGRTGTLGTLSRRHP
                     GHPFVSVMPYAPDERGRPLLLISTLAMHTQNLAADPRASLLVAQVGDDPLALARVTLM
                     GDARRLEPGERPAARDAYLVRHPNAVHWVDFDDFAFWCLDVSDVYFVGGFGAMDWLTV
                     PGYETAHPDPLADAAPGIIEHMNRDHADALVLYARVLGDLGSEAAGIVAAEMVAVDRL
                     GFKLRVRAADGLHGCRIAFPREAISAERCRAVLIEMLADLRSRPR"
     gene            complement(6086..7732)
                     /locus_tag="VFX87_02120"
     CDS             complement(6086..7732)
                     /locus_tag="VFX87_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4993729.1"
                     /translation="MLFTYLTTHFLNHSFGLISIDAMLAGREWFVLLWRSRLGTLALY
                     TAFTVHILLAYWSLYRRRTLRMPGWEATQLTLGLTIPPLLVWHVVGTRLAHEMAGTQD
                     SYTLVLLVLWKLDPLAGWRQATVLAVAWLHGCVGLHFWLRLRPWYPRFARAIYSGCLL
                     LPVLALLGFAQAGREISRLAQDPAFVPRVIAAAGLPQPEERVVLGRVSRSIFWGHLGL
                     IALILVTRATREHIRRRQGVRIAYPDGREVLVPAGFTILEASRLAGIPHASVCGGRGR
                     CSTCRVRVLRGAEALPAATADELRVLGRVGAPAHVRLACQVRPRRDLSVVPLVPAGVG
                     PGAAAGDGAGDHRDGRELSIAVLFADLRGFTRLAERKLPYDVVFILNRYFETVGGAIT
                     DVGGIVNQFTGDGVMALFGVDSGPEEGCRQAVRAAAAMVARLNEMSRTLADDLATPLR
                     LGIGIHTGPTVVGQMGYATTTYLTAVGDTVHVAARLEALTKEYGAELVLSEEVAVLAG
                     LDLSDHARHELTVRNREAPLAIFVAPSAGRLAERLAGVPA"
     gene            complement(7811..8611)
                     /gene="pcaD"
                     /locus_tag="VFX87_02125"
     CDS             complement(7811..8611)
                     /gene="pcaD"
                     /locus_tag="VFX87_02125"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEX4993730.1"
                     /translation="MKLKANGIDINYEIEGDGPVVTFSHSLACNLSMWDEQVRALRSR
                     YRVLRYDTRGHGQTGAPAGAYTLDQLADDLKGLLDGLGIRETHFVGLSMGGMIGQIFA
                     LKHPAMVQSLALCDTTSRYAPGAAATWEDRIKTVGAKGMEPMVAPTLERWFTAPFRAR
                     RKDLMERVGAMIRATSAAGYIGCCHALPKINATERLRGVRCPALVIVGEEDPGTPVEM
                     AREIHAALPAAELAVLCRASHLSNLEQPEEFNRVLGGFLDKLSGRTKL"
     gene            complement(8620..11526)
                     /locus_tag="VFX87_02130"
     CDS             complement(8620..11526)
                     /locus_tag="VFX87_02130"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX4993731.1"
                     /translation="MAEGARGAERILEGLNAPQAAAVTHETGPLLIIAGAGTGKTTVI
                     TRRIAYLIATRAARPSEILALTFTDKAAAEMEERVDTLVPYGYADVQIATFHAFGDRL
                     IKENALELGLTPDFRVLTRAEQIIFLRDHLFEFPLKHYRPLGDPTRHLHAILTQFSRL
                     KDEDVGPDEYLAHAEELLAAATDEESRLAAEQHLELARTYAQYQTLMARLGQIDFGDQ
                     IVEALRLFRTRPHILRRYQGRYRYILVDEFQDTNYAQFELVKLLAARHRNVAVVGDDD
                     QAIFRFRGASMSNILDFDRTYPDARQVVLQENRRSPQAVLDAAYRLIQHNNPDRLEVA
                     QKIDKRLISTVGVGEAPRHLPFDTVSTESDRVAELIREERERGRPYRDFAVLVRANND
                     ADHYLRALNMRGIPWTFSGNAGLYGRPEVRLLIAFLRSVVYTDDSISLHYLASSDVYQ
                     VPIVDLTNCATYANRKNRHLFDVLHDVPADLAVSDEGRLAIARLVTDLSRYMELGREH
                     PTGEVLYQFLVDSGLMTRYAKAPAELEQEVQNVSKFFTRVKDAARVLKYDNVREFVSH
                     LDALMDAGDDPAVAEADTETPAVHVLTVHKAKGLEWPIVFMVDCVQNKFPSTRRSDPL
                     EMPPGLIKDLLPTGDFHEQEERRLFYVGMTRAKERLYLTSAEDMGGKRKWKVSQFVLE
                     ALDLPKDAARPFRARAIEGLMRQAPPPELAGLGLAPIPDDEPLAVSHRQVDDYQTCPL
                     KYQFIHILRIPLRQHHSVVYGNALHKAVEFYLRRRAAGNYTSLEDFLLAFDNAWRNEG
                     FLTRDHEEQRKRAGVAALTRFYHEEEASGQKPTDVEREFGFTLGLDRVRGRFDRVDET
                     PDGLVIVDYKSSDVTDQKKADQRAKESLQLKIYALAQHAMTGRLPARVELRFLESGLV
                     GRHTPADPDLAKAREAIVEAARGIRRREFEATPEYQACRYCPYNQICPSTATQE"
     gene            complement(11561..12436)
                     /locus_tag="VFX87_02135"
     CDS             complement(11561..12436)
                     /locus_tag="VFX87_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX4993732.1"
                     /translation="MSRSAVLVHNPGSGQGRDRFHSITRMVDALRGRGITVTPQVTTG
                     PADATRLAVAALEARTDLVIVHGGDGTVNEAMQGMVGTSTPLAVWPGGTSNVLARELD
                     LPGGIDLLADVFAARRTRRVSVGRAGERYFLLMAGVGLDADIVRAVHPGLKRLTGEGA
                     YWMAGLRQLTDWHPVPFLVETDAGQHSATFAVVANAASYGGGLRFAPEARMDDDLLDI
                     CLFDSTQRHRFARYLAAAFTGSHLGMPGVTYVKARRAVVHGDASRFVQVDGELLGPLP
                     MSFECVPDALSLVVP"
     gene            complement(12446..>13450)
                     /locus_tag="VFX87_02140"
     CDS             complement(12446..>13450)
                     /locus_tag="VFX87_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX4993733.1"
                     /translation="QGARDGHRVSRPEPRHDLVGLGEVMLRLAARPPQRLEQAQDLDV
                     QIGGTEANVAAACARLGLRTAFISVLPSEHAWGDRTIRELSGHGVDCGGVLRRPGQRM
                     GLYFIEYGMAPRPVRILYDRRDSAFSRLVADDVDWTLIRSARLVHLTGVTAALGENIR
                     EVVRRAIAEAEAARVPVSFDVNYRSRLWSPKEARDFLGEILPRLGYLFIGSDDAATVL
                     ELEGSPERVLDGLRRLAPSATIALTLGEAGSAVLTERSVYRPSKLYTVSVVDRVGAGD
                     AFAAGFLWATLTGRDAQRAVDAATALAALKCTIWGDVPVVTRAELDELLATDSTEIRR
                     "
BASE COUNT         1919 a         4743 c         4896 g         1892 t
ORIGIN      
        1 cgagcggagt gggccgggtt gaggggaggc taggacagcg cgggccggcg ggtgtgccag
       61 tcggtggctt gctggtaggc gtcgcccagg cggaggacgg tgggctcgtc gaaggggcgg
      121 cccacgatct ggtaggccag cggcaggccg gccgaggcga agccgcaggg cacgctgagc
      181 gcgggcaggc cgagcccgtt gaagggccgc gtgaggcgcg agaagcgccc ctggcgtgcc
      241 acgagctcgt gcacggggcc cacggtggcg tgggccagcg agggtgcggc ctccggaatc
      301 accggcgcca ccacgatgtc cacttcggcg aacacctggg cgatgaacgc gcgggtgaga
      361 cgggcgcgga gccggagcgc ctgcaggtag tcgtaggccg gaacctgcat ccccagctcg
      421 agccgcgagc gcacgaccgg ctggatctcg tgcggacgct cgcggagcag ctgcgcgtgg
      481 atggtcgagg cctcggagcg gctgacgatg ttcgtgacgt cgctcatggt ctgcgggtcc
      541 ggcacgcgga gctcggagac gcgggcgccg agcgtcgcga tcacgtccac cgcccgccgt
      601 accgcggcct ccatctcggc gtcgatgccc tggaagaagt agttctccac tacgccgacc
      661 cgcagacctg cgacagtccg gcccagcgcg gagacgtagt cgggcaccgg ctccgggctc
      721 gaggtcacgt cgagcgcgtc ccggcccgcc atcacctgca ggagcagcgc gcaatcccgg
      781 acggtacgcg ccatcggccc cacgtggtcg ttcgaccagc tgagcggcat ggcgcccgct
      841 cggctcacgc gaccgtaggt cggcttgagc ccgacgatgc cgcaggccgc cgcgggcagg
      901 cggatggacc cgccggtatc gctgcccacg gcccccaggg cgaggcgtcc cgccaccgcg
      961 accgcggagc cgctcgagga gccgccggtg cagtgcccgg cccgccacgg attctgggcg
     1021 tgcccgtggt gggcgttgtc gccgaagggc cccatcgcca gctcggtcat gttgagcttg
     1081 cccagcgtga ccgcgcccgc ggccgcgagg cgcgccacaa ccgtacagtc ctgctcggcc
     1141 tggaagtact cggccgtgct ggtcccgcag gaggtcggca gcccgcggat gaagcagagg
     1201 tccttgaagg cgagcggcac gccgtggagg ggccccctcc agacgccccg cccctgctcg
     1261 gcctcgagcc ggcgggcggt ctcgagcgct ccggcgtgat ccaccgtgat gaaggcgcgg
     1321 agaccgccat cgaggcgctc gatccgcgcg agacactccc gggtggcctc gaccggcgag
     1381 atcttgcccg cgcggatcgc ggtcgcgagg ccctcgaggg tccagtcggt cactcgggat
     1441 cgcctcccgc cggcgggcgg cccgcctcga gagccgcgat gaagcgcgcc ggctcctcgg
     1501 ccagcgccag atcggcggcc atgcgctcga tgacggcgag gagagccgcc gcctcggcct
     1561 gctgcaccct ggactcgtcc atggactcgc gtcccccttt cacgaagccg gcgccagcaa
     1621 ggcggtcagg tcgcgtacgt ccttgagcgt ctcgacctga tctacgagct ccgccaccgc
     1681 gcgagcgcgg tcggcgccca gcacgtcggt caccagcggg atgaacttcc cgacaacctc
     1741 cccggccgaa accggatccg cgaagtctcc gcgcaccacc gtggtggagg cggacagcgt
     1801 gcgcccatcc cggagccgca cctcgacaag cgccgtcggg tagtccaccc ggcgcggcga
     1861 catccgcggg tccacgcgca cctccactcg cgcggccagg gatcgaatgc gcgggtcgga
     1921 gagcgccggc tccacgaacg cggcgacgtc ggcacggccc agcacgagcg cggccgccac
     1981 cgcgtacggg atcgagaact tcgcccccag catggacgcg ggggcgggat cggccatgcg
     2041 caggccgaac gggatcgtgg tcaccaccac gcgctcgacc tgggcggcct cgagccgcgc
     2101 cgccctcgtc atcgccagca gcgcgtcgag cgcgaagtgg ttgtagcggc agcaggcgta
     2161 gagcttgaag tagttctgct cgatgcgcag cgggccgccg aggccgtcca gcgcgcgctc
     2221 gggctcgaag cggtcggcga ggatggtgcc gtagacgtcg cgcggcgcgt cggccaggcc
     2281 ggtgaatccc gcgcgatgga ggtccaccgc caggagcccc tcgagcccgg agcgcccggg
     2341 gtaggcgttg cggatcgtcg cgccctcgag tgccgtggtc caggtattgg cgggactcat
     2401 gctcgcggag agattgatca ccgaccgcac cccggccgcg tcgaggccgg ccagcctggc
     2461 cgccgccacc gcggtgccga tggtgcccca ggtgccgtgg gagtggacgt tcggcttcgg
     2521 cgtcgtcgca ccgccgaggc gcgagcagac ctcgtagccg gcgatgatgc tctcgagcag
     2581 ccggcggccg tcggcgccgg tgtcctccgc caccgcgagc gcgccgggga ccacgtgaat
     2641 ggccgggtgc ccaccgccca ggcggctccc ctcgtccact tccagcgcca ccccggcggt
     2701 cgcgttgacg aacgtggcga ggagcgggtc cgccttgccg ccgtggccca ggagcgtcgc
     2761 ggtggcgcgc gagcgccgct cgtgcatggc ctcggcgagg cgccggttct ccggctgcgc
     2821 gctgcccgcc accatcgcgc cgatcgtatc gagcagcacg agccgcgccg cgcgcagcgc
     2881 gtcgtcggtc aggcgctcgg cggccacccc ctcgacgaac gccgcgagcc ggtcgaggta
     2941 gtccgtcatc gcgaggcgcc taccccgcgc cgcccgcgat cggcgcgacc gggccggtgc
     3001 gcgccaggta gcggccgtag cccggcttct gctcgagccg gccctgattc agcaggacct
     3061 ggccccgcag caccgtcatc cagggccgcc cgcgcgactt ccagccctcg tagagcgtcc
     3121 agcccgcgat cccgtgatga tcggccacct tgatcgtccc gtcggagtcg ggatcgatga
     3181 tggtcagatc ggcgtcggcg cccacccgga tcgcgccttt gcgtggatag aggccgaaga
     3241 tccgcgcggg attctcggcc atcacgcggg ccagccacgt cacggggagg ccgcgcttca
     3301 ccacgccctc gctccacatg agcggcacca gcgtctcgat ggacgggctg ccgaacggga
     3361 ccggattgcc cttgccgtcc acgaagatgt tcttccagcc cggctccttc agctccttgc
     3421 ggcgcggcga gtgatcgctc gccaccgtgg cgatgtagcc ttcgcggctg ccgccccaga
     3481 gcgcgtcgcc gtcaccgccg cccaccgggc ggagcggcgg gccgatcttg gcgagcggcc
     3541 cccaggcctc catctccttc tcggtgagca gcatgtactg cgggcaggtc tcggtccaca
     3601 cgcgctggcc gcgggcctgg gcttccttga ttcgctcgag cccgcccttg gtcgagaggt
     3661 ggacgacgta gaccgggcac tgggtcatct cgccgatgag aatggcgcgg ttgatcgcct
     3721 cctcctcggt ccacggcggg caggtcgccg ggaaatcggt gggcttcacg cgccccgccg
     3781 cgatggactt gttctcgagg tggtagagga tgtcgccgtt ctcgcagtgg agctggcaga
     3841 caccgcccag cgccgcgatc cgctccatga cctgggcgat gaactcgtcc gagcacatgc
     3901 ggttgggccg ccgcttgtag gtcatgaaga tcttgtagga cgtgacgccc tgctcgaagg
     3961 cctcggccag cccgtccagg atgtagggct ggttgttcag gatgaagtgg aagccgaaat
     4021 ccaccaccga ctgctgcccc gcctcgtcgc gcagacgctt gagcgcctgg ggcagcgtct
     4081 cccgcgcctg gtcgtcgtac tccacgaacg agagcagcgt cgtcaggccc gcgtgggcgg
     4141 cggccaccgg cccggtgtgc cagtcgtcgt agaccgcgcc cagatggacg tggcagtcga
     4201 tcaagccggg cagcacgtac ttgccggtgg cgtcgatcgt gcgctcggcg ggcggcagca
     4261 tggcctcggc ccccagcgcg acgatttgcc cgtccttcac cgcgaccgcg gtctccagca
     4321 cctccgtcgg agtaacgacc cggccacccc gcacgatcag gtcgatcgaa gagctcttcg
     4381 cgcgagcgct catcgcgcgc ccgacctggt gggttgagtc aggaggccgt agtgttcggg
     4441 gtgccggcgg ccgaggaagt cccagcgctt gcgcgccggc accatctggt cgaggtcgat
     4501 ccgggcggcg accagctcgt cgccggtggt cgcggccttg gcgaccacct gccccatcgg
     4561 gttcacgatg cacgagccgc cgatcagctc catgccgtct tcgacgccgg ccttggcgat
     4621 gccgaccacg aacaggttgt tctggtaggc acccgcgcgc aggcacagct cgttcagatc
     4681 gagggccagc ggcgagagcg gcgtgttgta gccgatcagc acgatctcgg cgcccagcag
     4741 gccgaggctg cggtaggact ccgggaagcg ccggtcctgg cagatcgcga cgccgacctt
     4801 ggcgcgcgcg gcgtcgaaga cccggtagcc gctattaccc gactcgaaga agtgcggctc
     4861 gtagacctgg gcgtggccgt ccggctgctt cgcgcccggc agatggatct tccggaaggt
     4921 ccccgcgagc ttgccggtct cgtcggtcaa cagcgcggta ttgaagtagc ggccctggtc
     4981 cttctcgcag aagccgacgt ggcaggcgat gcgcgcctcg cgggcccgct gcagcagcgg
     5041 agccagcgcc ttgggcggca tctcggtctc gaagaactgg tcgaagtcgt cgcgcacgcg
     5101 cttggggaag taggtcgtga gcgccatctc ggggtatgcc aggatctcga cgccttcgac
     5161 gatggcctgg tcgaggaggc cgagcatgcg ctcgacgatc tcctcgcggc tcgtcccttc
     5221 gttgttgggg cccatctgcg cggcggcgac cttgatgtgg cgtgccatgg ggtcctctcc
     5281 tttcgggagt acatacgtca ccgcgggcgg ctgcgcaagt cggcgagcat ctcgatcagg
     5341 acggcccggc accgctcggc gctgatcgcc tcgcgcggaa aggcgatccg gcacccgtgc
     5401 aggccgtcgg cggcgcggac gcgcagcttg aagccgaggc gatccaccgc gaccatctcc
     5461 gccgcgacta tcccggccgc ctccgagccg aggtccccga gcacgcgggc gtagaggacc
     5521 agcgcgtcgg cgtggtcgcg gttcatgtgc tcgatgatgc cgggggcggc gtcggccagc
     5581 ggatcggggt gggccgtctc gtacccgggc accgtgagcc agtccatcgc gccgaagccg
     5641 ccgacgaagt agacgtcgct gacgtccagg caccagaacg cgaagtcgtc gaagtccacc
     5701 cagtgcaccg cgttcgggtg gcgcacgagg taggcgtcgc gcgcggcagg ccgctcgccc
     5761 ggctcgaggc ggcgggcgtc gcccatgagc gtgacgcggg cgagcgcgag cggatcgtcg
     5821 ccgacctggg ccaccagcag gctcgcgcgc ggatcggccg ccaggttctg ggtgtgcatc
     5881 gcgagcgtgc tgatgagcag gagcggccgg ccgcgctcgt ccggcgcgta gggcatgacc
     5941 gagacgaacg ggtggcccgg gtggcggcgg gagagcgtgc cgagcgtgcc ggtgcgcccc
     6001 aggtgcacga gcgtgcgcgc ctgctccgcg aacgtgggct cgggcgcgcc cggccccgcg
     6061 gggcgcccgc tgtggccgtg gcggctcatg cgggcacgcc cgctagccgc tcggcgaggc
     6121 gccccgcgct cggcgccacg aagatggcca gcggtgcctc gcggttgcgc acggtgagct
     6181 cgtgccgcgc gtgatcggac aggtcgagcc cggcgaggac cgcgacctcc tcggagagca
     6241 cgagctcggc gccgtactcc ttcgtgagag cctcgaggcg cgccgccacg tgtacggtgt
     6301 cccccaccgc ggtcaggtac gtggtcgtcg cgtatcccat ctggcccacc acggtggggc
     6361 cggtgtggat cccgatcccg aggcgcagcg gcgtcgcgag gtcgtcggcc agcgtccggc
     6421 tcatctcgtt caggcgcgcg accatggcgg cggcggctcg gaccgcctgg cggcagccct
     6481 cctccggacc cgagtccacc ccgaagagcg ccatcacccc gtcgccggtg aactggttga
     6541 cgatgccgcc cacgtcggtg atcgcgccgc ccacggtctc gaaataccgg ttcaggatga
     6601 agaccacgtc gtacggcagc ttgcgctccg cgagccgggt gaatccacgg aggtcggcga
     6661 acagcaccgc gatggagagt tcgcgcccgt cgcgatggtc gcccgcaccg tcgccggccg
     6721 cagcgccggg gcccaccccc gccgggacga gcggcaccac cgagaggtcc cgccggggcc
     6781 ggacctggca ggcgaggcgc acgtgggccg gggcgcccac tcgcccgagc acgcgcagct
     6841 cgtcggcggt cgcggcgggc agggcctcgg cgccgcgaag cacgcgcacg cggcaggtgg
     6901 agcagcgccc gcggccgccg cacaccgagg cgtgcgggat ccccgcgagg cggctcgctt
     6961 cgagaatcgt gaagccggcg ggcacgagca cctcacgccc gtcaggatag gcgatccgca
     7021 cgccctggcg ccggcgaatg tgctcgcgcg tggcccgggt gaccaggatc agggcgatca
     7081 ggcccaggtg cccccagaag atcgagcgcg acacgcgccc gagcaccacg cgctcctccg
     7141 gctgcggcag ccccgccgcc gcgatcaccc gcgggacgaa ggccgggtcc tgcgccaggc
     7201 gcgagatctc gcgtccggcc tgcgcgaagc ccaggagcgc cagcaccggc aggagcaggc
     7261 acccgctgta gatcgcccgc gcgaaccgcg gataccaggg ccgcagccgg agccagaagt
     7321 gcaggcccac gcagccgtgc agccacgcga ccgcgaggac ggtcgcctga cgccatcccg
     7381 ccagcggatc gagcttccag agcaccagca acacgagcgt gtagctgtcc tgcgtgccgg
     7441 ccatctcgtg ggcgagccgg gtgcccacca cgtgccacac gagcaggggc gggatcgtca
     7501 ggccgagggt gagctgggtc gcctcccacc cgggcatacg aagcgtgcgc cgccggtaga
     7561 gcgaccagta ggcgagcagg atgtggacgg tgaacgcggt gtagagcgcg agggtgccga
     7621 gccggctccg ccagagcagg acgaaccact cgcggcccgc cagcatggcg tcgatcgaga
     7681 tgagtccgaa gctgtggttc aagaagtgcg tggtgaggta cgtgaacagc acgaggcccg
     7741 cccagaggcg aatctgccga acgacggccg ccgctgagcg ggccgggggc atcgagcggg
     7801 tcgcgctccc ctacagcttg gtccggccgc tcagcttgtc gaggaagcct ccgagcaccc
     7861 ggttgaactc ctccggctgc tcgagattcg acaggtgcga agcgcggcac agcacggcca
     7921 gctctgccgc gggcagcgcg gcgtggatct cgcgcgccat ctccaccggc gtgccgggat
     7981 cttcctcgcc cacgatcacc agcgcggggc agcggacgcc gcggagccgc tcggtggcgt
     8041 tgatcttcgg gagcgcgtgg cagcagccga tgtagcccgc ggcggacgtg gcgcggatca
     8101 tggccccgac gcgctccatc agatccttgc ggcgcgcgcg aaagggcgcg gtgaaccagc
     8161 gctcgagcgt cggggccacc atgggctcca tgcccttggc gccgaccgtc ttgatgcggt
     8221 cttcccacgt cgcggcggcg cccggagcgt agcggctcgt ggtgtcgcag agcgcgaggc
     8281 tctgcaccat cgcggggtgc ttgagcgcga agatctgccc gatcatgccg cccatcgaga
     8341 gccccacgaa gtgggtctct cgaatgccga gcccgtcgag gagccccttc aggtcatcgg
     8401 cgagctggtc gagggtatag gcccccgcgg gcgcgccggt ctgcccgtgg ccgcgcgtgt
     8461 cgtagcgcag gacgcggtag cggctcctga gcgcgcgcac ctgctcgtcc cacatggaca
     8521 ggttgcaggc gagcgagtgg ctgaacgtca ccaccgggcc gtcgccctcg atctcgtagt
     8581 tgatgtcgat gccgttggcc ttgagcttca tggctctcct cactcctggg tggcggtgct
     8641 cgggcagatc tggttgtagg gacagtagcg gcaggcctgg tactccgggg tcgcctcgaa
     8701 ctcccggcgc cggatgccgc gcgccgcctc cacgatggcc tcgcgagcct tcgccaggtc
     8761 cgggtcggca ggggtgtggc gcccgaccag ccccgattcg aggaagcgca gctccacccg
     8821 cgcggggagg cggccggtca tcgcgtgctg cgcgagcgcg tagatcttga gctggaggct
     8881 ctccttcgcg cgctgatcgg ccttcttctg gtcggtgacg tcgctcgact tgtagtccac
     8941 gatcacgaga ccgtccggcg tctcgtccac ccggtcgaag cgcccgcgca cccggtccag
     9001 gccgagcgtg aaaccgaact cccgctccac gtcggtgggc ttctgccccg aggcctcctc
     9061 ctcgtgatag aagcgcgtca gcgcggcgac gcccgcccgc ttgcgctgct cctcgtggtc
     9121 gcgggtgaga aagccctcgt tgcgccaggc gttgtcgaag gcgaggagga agtcctcgag
     9181 cgaggtgtag ttgcccgcag cgcgccggcg caggtagaac tcgaccgcct tgtgcagcgc
     9241 gttgccgtag accaccgagt ggtgctggcg cagcgggatg cgcaggatgt ggatgaactg
     9301 gtacttgagc gggcacgtct ggtagtcgtc cacctgccgg tggctcaccg ccagcggctc
     9361 gtcgtccggg atcggggcga gcccaaggcc cgccagctcc gggggcggcg cctgccgcat
     9421 cagcccctcg atggcgcgcg cccggaaggg gcgcgccgcg tccttcggca ggtcgagtgc
     9481 ttccagcacg aactggctca ccttccactt gcgcttgccg cccatgtcct ccgcgctggt
     9541 gaggtagagg cgctccttgg cgcgggtcat gcccacgtag aagaggcgcc gctcctcctg
     9601 ctcgtggaaa tcgccggtgg gcaggagatc cttgatgagc ccgggcggca tctcgagcgg
     9661 atcggagcgg cgggtggacg ggaacttgtt ctgcacgcag tccaccatga acacgatcgg
     9721 ccactcgagc cccttggcct tgtgcacggt gagcacgtgc accgcgggcg tctcggtgtc
     9781 ggcctcggcc accgccggat cgtcgcccgc gtccatcagc gcatccaggt gactcacgaa
     9841 ctcgcgcacg ttgtcgtact tgagcacccg cgcggcgtcc ttgacgcggg tgaagaactt
     9901 gctcacgttc tgcacctcct gctccagctc ggcgggcgcc ttcgcgtagc gggtcatgag
     9961 cccggagtcc accaggaact ggtagagcac ctcgccggtc gggtgctccc ggcccagctc
    10021 catgtagcgg ctcagatccg tcaccagccg ggcgatcgcg aggcgccctt cgtcgctcac
    10081 cgccaggtcg gcggggacat cgtgcagcac gtcgaagagg tggcgattct tgcggttcgc
    10141 gtacgtcgcg cagttcgtga gatccacgat cggcacctgg tagacgtcgg aggacgcgag
    10201 gtagtggagg ctgatcgagt cgtcggtgta gacgacgctg cggaggaagg cgatcagcag
    10261 gcggacctcg gggcggccgt agaggccggc gttgcccgag aaggtccacg ggatcccgcg
    10321 catgttgagc gcgcgcaggt agtggtccgc gtcgttgttg gcccgcacca gcaccgcgaa
    10381 gtcgcgatag ggccggcctc gctcgcgctc ctcccggatg agctcggcca cccggtcgga
    10441 ctcggtggag acggtgtcga aggggaggtg ccgcggcgcc tcgcccaccc ccacggtgga
    10501 gatgagccgc ttgtcgatct tctgagccac ctcgaggcgg tcgggattgt tgtgctggat
    10561 gagccggtag gccgcgtcca gcaccgcctg gggcgagcgg cggttttcct gcagcacaac
    10621 ctggcgcgcg tccggatagg tccggtcgaa gtcgaggatg ttggacatgg aggccccgcg
    10681 gaagcggaag atggcctgat cgtcgtcgcc caccaccgcc acgttgcgat gccgggccgc
    10741 cagcagcttc accagctcga actgcgcgta gttcgtatcc tggaactcgt ccaccaggat
    10801 gtaccggtac cgcccctgat agcgccgcag gatgtgcggg cgggtgcgga agaggcggag
    10861 cgcctcgacg atctggtcgc cgaagtcgat ctgcccgagc cgggccatca gcgtctggta
    10921 ctgcgcgtag gtccgcgcga gctcgagatg ctgctcggcc gcgaggcgcg actcctcgtc
    10981 ggtcgcggcg gccagcaact cctcggcatg cgccaggtac tcgtccgggc ccacgtcctc
    11041 gtccttcagg cggctgaact gagtgaggat ggcatggagg tgccgggtcg ggtccccgag
    11101 cggccggtag tgcttgagcg gaaactcgaa gaggtggtcc cgcaggaaga tgatctgctc
    11161 ggcccgggtc agcacccgga agtccggggt gagccccagc tcgagggcgt tctccttgat
    11221 caggcggtcg ccgaaggcgt ggaacgtggc gatctgcacg tcggcgtagc cgtagggcac
    11281 gagggtgtcc acgcgctctt ccatctcggc cgccgccttg tcggtgaagg tcagcgcgag
    11341 gatctcggaa gggcgcgccg cgcgggtcgc gatgagatag gcgatgcggc gggtgatgac
    11401 ggtggtcttg ccggtgcccg cgccggcgat gatgagcagc gggccggtct cgtgggtgac
    11461 ggcggccgcc tggggcgcgt tcagcccttc gaggatacgc tcggcgcccc gcgctccttc
    11521 cgccatcagg cgagactata ccaccgctgg aacccgcgct ctacggcacc accagggata
    11581 gcgcgtcggg cacgcactcg aagctcatgg gcagcgggcc gagcagctcg ccgtccacct
    11641 gcacgaagcg gctcgcgtcg ccgtgcacca ccgcgcggcg cgccttcacg taggtcacgc
    11701 cgggcatgcc caggtgcgag ccggtgaagg ccgcggccag gtagcgggcg aagcggtggc
    11761 gctgggtgga gtcgaagagg cagatgtcga gcagatcgtc gtccatccgc gcctccgggg
    11821 cgaagcgcag cccgccgccg taggacgccg cgttggccac caccgcgaaa gtggcgctgt
    11881 gctggccggc gtcggtctcc accaggaagg gcaccggatg ccagtcggtg agctggcgga
    11941 gccccgccat ccagtaggcg ccctccccgg tcaggcgctt gagccccggg tggaccgccc
    12001 gtacgatgtc ggcgtccagg ccgaccccgg ccatcagaag gaagtagcgc tcccccgccc
    12061 ggccgaccga cacgcgccgc gtccgcctcg ccgcgaagac gtcggcgagc agatcgatgc
    12121 cgcccggcag gtcgagctcc cgggccagca cgttggacgt gccgcccggc cacaccgcga
    12181 gcggggtgga cgtgccgacc atgccctgca tggcctcgtt cacggtgccg tcgcccccgt
    12241 ggacgatgac caggtccgtc cgcgcctcca gcgccgccac ggcgagccgc gtggcgtcgg
    12301 ccggcccagt ggtcacctgc ggggtgaccg tgatgccgcg cccgcggagc gcatccacca
    12361 tgcgcgtgat cgagtggaag cgatcgcggc cttggccgga gccgggattg tggacgagga
    12421 cggcggagcg cgacacggcc cctcgctagc ggcggatctc ggtgctgtcc gtggccagca
    12481 gctcgtcgag ctcggcccgg gtcaccacgg gcacgtcgcc ccagatcgtg cacttgagcg
    12541 cggccagcgc ggtcgcggcg tccaccgccc gctgcgcatc gcggccggtc agcgtggccc
    12601 agaggaaccc ggccgcgaag gcatcgcccg cccccacccg gtccaccacc gagaccgtgt
    12661 agagcttgga cggccgatag acgctccgtt cggtgagcac cgcggacccg gcctcgccca
    12721 gcgtgagcgc gatggtggcc gagggcgcga ggcgacgcag cccgtccagc acgcgctcgg
    12781 gcgagccctc cagctcgagc acggtggccg cgtcgtccga gccgatgaag agatagccga
    12841 ggcgcggcag gatctcgccg aggaaatcgc gcgcctcctt cgggctccag agccgggagc
    12901 ggtagttcac atcgaaggac acgggcacgc gcgccgcctc cgcctccgcg atcgcgcggc
    12961 gcaccacctc gcggatgttc tccccgagcg cggcggtcac gccggtgagg tgaaccagcc
    13021 gggcgctccg gatcagcgtc cagtccacgt cgtccgccac gaggcggctg aacgcggagt
    13081 cgcgccgatc atagaggatg cggaccgggc gcggcgccat gccgtactcg atgaagtaga
    13141 gtcccatgcg ctggcccgga cggcgcagca ccccgccgca gtccaccccg tggcccgaca
    13201 gctcgcggat ggtgcggtcg ccccacgcat gctcggaggg caggaccgag atgaaggcgg
    13261 tgcggagccc caggcgggcg caggccgcgg ccacgttggc ctcggtaccg ccgatctgga
    13321 cgtccaggtc ctgggcctgc tcgagccgct gcggcgggcg ggcggccagc ctcagcatca
    13381 cctcgccgag gccgaccaga tcgtgccgcg gttcgggccg gctcacgcgg tgcccgtcac
    13441 gcgcaccttg
//
LOCUS       DASUFA010000056         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_127635, whole genome shotgun sequence.
ACCESSION   DASUFA010000056 DASUFA010000000
VERSION     DASUFA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127635"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFX87_02145"
     CDS             <1..310
                     /locus_tag="VFX87_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX4993734.1"
                     /translation="SDQIPGDAIPFDMAGVDFGHHGEDCTFETLLRRTGLRDRKLALL
                     AEIVHEADIKDQKYAREEARGIDLALRALLSVIKDDHEALAQGMTLFDGLYATVGERA
                     "
     gene            307..1476
                     /locus_tag="VFX87_02150"
     CDS             307..1476
                     /locus_tag="VFX87_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX4993735.1"
                     /translation="MSEPRTTWGLVRYFLYLGSLGFGGPVALIGYMQRDLVERRSWFT
                     KPDYFKGLTLSQLAPGPLAAQLAMCLGYVHGGAAGATLIGLTFILPSFLMTLALGAAY
                     VAYGGLGWMQAAFYGVGAGVIGIIVRSAWKLLRLSLGADRLLWAIAGSMALVTVWTET
                     EIGSLFILCGLVVLLVQAPPRRLLAWAGSSKSLPAVGWPLILLTGLTATTTPGVLAQI
                     LWFFTKAGAFVFGSGLAIVPFLYGGVVQEYRWLTDKQFLDAVAVAMLTPGPVVITVAF
                     IGYLVAGVAGGTAAAIGVFLPTYLFVVIPYRWFDRFGENPQVKAFVNGVTAAAAGAIA
                     GACVVLGRRAIFDLPTALIGLAAFGLLWRLKVAEPILIAAAGVTGLIIFWIRGAL"
     gene            1473..1889
                     /locus_tag="VFX87_02155"
     CDS             1473..1889
                     /locus_tag="VFX87_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993736.1"
                     /translation="MSTPRSVLFVCLHGAAKSVLAAADFRRLAAERGLALTALSAGTE
                     PDPEIAPGVVAALRAEGVELGQTRPRQVTAADTALADRVVTFGCELGEATPAAVAVER
                     WDDVPAVSENLPLARAAIRRHLDRLLDEYETRGARR"
     gene            1891..2034
                     /locus_tag="VFX87_02160"
     CDS             1891..2034
                     /locus_tag="VFX87_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993737.1"
                     /translation="MGDAAQSTGGPEFLFVAEDEVMDTVENMALQFPVYDALYAYCRA
                     DRA"
     gene            2031..3182
                     /locus_tag="VFX87_02165"
     CDS             2031..3182
                     /locus_tag="VFX87_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4993738.1"
                     /translation="MSRDGRLVFVAKAARTFCYGFLGVLFPVYLAERGLDAREIGVAV
                     TLTLLASAAMTFAIRRPAERYGARAALVAQAGLSVVSALVFLATEQPWLIVAAAMMGN
                     LAVGTGETGPFLTLEQVAVTRATPRERLTTALSLYNLAGYAASALGAAAVGYAGPSPR
                     LLFAVFLLGGLVQMGAYALMRPDPGRSAVGRHTTPMPSRALVRRLAALFALDSLAGGF
                     VLQSLIAYWLYTRFEMGLAALGWTFFGAQVLSGLSLLLAAKLAPRLGLVNTMVFSHLI
                     SNVLLIGVALAPSAGLAVTLLLARHLLSQMDVPTRQSFLMLAVQDHEREGAASLTNMS
                     RTLAQSVSPALTGWVMASVSLAAPFFLGGGLKIAYDLMLYATIRRLETR"
     gene            3179..4162
                     /locus_tag="VFX87_02170"
     CDS             3179..4162
                     /locus_tag="VFX87_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX4993739.1"
                     /translation="MTRLLTRGDVAALLTLDDCIGAVEQGFALQGAGRAFGPGVLGLH
                     VPDGGFHVKAAGLPLSRLYVAVKLNANFPANGRRRGLPTIQGVLALSDGETGQPLALM
                     DSMEITVLRTGAATAVAARHLARPDARVATIVGCGHQGRIQLAALARVRPLERAYAVD
                     TDTTVAQRFAEETSARLALDVRPAGSVADVAPLSDICVTCTPSRTPLLRREHVAGGTF
                     IAAVGADNEDKVEIDPALMAAGTIVVDSLEQCATIGDLHHALALGVVSMADVHAVLAD
                     VVVGRKPGRTSADEITIFDSTGVAIEDVAAAAVVYERALALGRGLTIDLAG"
     gene            complement(4169..4951)
                     /locus_tag="VFX87_02175"
     CDS             complement(4169..4951)
                     /locus_tag="VFX87_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993740.1"
                     /translation="MGDPRGRVALVTGASSGIGAATARVFAGAGLRVALCARRKDRLE
                     QLAAELTARGTEAAVHAVDVTDPLAVRAMVDEVAARWGRLDVLVNNAGRGFAATLEQT
                     TAAELRALMELNVMAALGATQAALPIMRRQGSGHVINISSVVGRRAVPYRSAYSATKF
                     ALGALSEALRVELTGTGIAVTLVYPIRTDETEFHEAEVRKVPWRPMGPVQSAERVARS
                     ILRCVRRPRPEVYPYWPVRILAVMSVIAPAVVDRGMRRVLQR"
     gene            complement(4944..>5358)
                     /locus_tag="VFX87_02180"
     CDS             complement(4944..>5358)
                     /locus_tag="VFX87_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX4993741.1"
                     /translation="PMTSKLEVPQAPASRARAVLIYDGTCGLCRGGVSWLSRRAVSGH
                     LEFLPCQAAERRARYPWMGEGTCLQAMQLILPDGRVLAGDAAIPEILRRLRGWRWLAQ
                     AFRLPGMELLAPRLYGWVARHRYQISCMLGRSRRG"
BASE COUNT          690 a         1996 c         1852 g          820 t
ORIGIN      
        1 gtccgatcag attcccggcg acgcgatccc gttcgacatg gccggcgtgg acttcggaca
       61 tcacggcgag gactgcacgt tcgagaccct cctgcgccgc accgggctcc gcgaccgcaa
      121 gctggccctc ctggccgaga tcgtccatga ggccgacatc aaggaccaga agtacgcgcg
      181 tgaggaagcg cgcggcatcg atctcgcgct gcgcgcgctg ctgagcgtga tcaaggacga
      241 ccatgaggcg ctggcgcagg gaatgacatt gttcgacggc ctctacgcca ccgtcggaga
      301 gcgagcatga gcgagccgcg cacgacctgg ggcctcgttc gctatttcct ctacctgggc
      361 agcctcggct tcggcggccc ggtggcgctc atcggctaca tgcagcgcga tctcgtcgag
      421 cgccgctcgt ggttcaccaa gccggattac ttcaagggcc tgaccctttc ccagctcgcg
      481 cccgggcccc tcgccgccca gctcgcgatg tgcctcggct acgtccacgg aggggccgcg
      541 ggcgccaccc tgatcgggct caccttcatc ctcccctcct tcctgatgac gctggccctg
      601 ggcgcggcct acgtcgccta cggcggcctc ggatggatgc aggcggcctt ctacggagtg
      661 ggcgcgggcg ttatcggcat catcgtccgc tcggcgtgga agctcctgcg gctgtccctg
      721 ggagccgacc ggctgctctg ggcgatcgcc ggctcgatgg ccctcgtcac ggtgtggacc
      781 gagaccgaga tcggcagcct cttcatcctg tgcggcctcg tggtcctgct ggtgcaggcg
      841 ccgccccgcc gcttgctggc ctgggccggc tcgagcaaga gcctgcccgc agtgggctgg
      901 cccctgattc tgctgaccgg cctgaccgcc accacgacgc ccggcgtgct ggcccagatc
      961 ctctggttct tcacgaaggc gggcgccttc gtattcggca gcggcctggc catcgtgccc
     1021 ttcctctacg gcggggtcgt gcaggagtat cggtggctca ccgacaagca gttcctcgac
     1081 gcggtcgcgg tggccatgct gacgccgggg ccggtggtga tcacggtggc cttcatcggc
     1141 tatctcgtcg cgggcgtggc gggcggcacc gcggccgcca tcggggtctt cctgcccacc
     1201 tatctcttcg tcgtcattcc ctaccgctgg ttcgaccgct tcggcgagaa cccgcaggtg
     1261 aaggccttcg tcaacggcgt gaccgccgcc gcggccggcg ccatcgcggg cgcctgcgtc
     1321 gtcctcggcc gccgcgccat cttcgacctg ccgaccgccc tgatcgggct ggccgcgttc
     1381 ggcctgctct ggcgcctgaa ggtggccgag ccgatcctga tcgcggcggc gggtgtcacc
     1441 ggtctgatca tcttctggat acgaggagcc ctatgagcac accacggtct gttctcttcg
     1501 tctgcctgca cggcgcggcc aagagcgtcc tcgccgccgc cgacttccgg cgcctggccg
     1561 cggagcgcgg ccttgccctc accgccctgt cggcgggcac cgagcccgat cccgagatcg
     1621 cgcccggcgt ggtcgcggca ctgcgagcgg aaggggtcga gctcggccag acccggccgc
     1681 gacaggtcac cgcggcagat acggcattgg ccgatcgcgt cgtcacgttc ggctgcgagc
     1741 tcggcgaggc gacgccggcg gcggtggcgg tggagcgctg ggacgatgtg cccgcggtga
     1801 gcgagaatct gccgctcgcc cgcgccgcga tccggcgcca tctcgaccgc ctgctggacg
     1861 agtacgagac ccggggagca cgccgatgaa atgggtgacg cggcgcaaag cacgggtgga
     1921 ccggagttcc tgttcgtggc ggaggacgag gtgatggaca ccgtcgagaa catggcgctc
     1981 cagttcccgg tctacgacgc gctctacgcc tactgccggg ccgatcgcgc gtgagccgcg
     2041 acgggcgcct ggtcttcgtc gccaaggcgg cgcgcacctt ctgctacggc ttcctcggtg
     2101 tcctcttccc cgtctacctg gccgagcgcg gcctcgacgc gcgggagatc ggggtggcgg
     2161 tgacgctcac cctgctcgcc agcgcggcca tgaccttcgc gattcgccgg cccgccgagc
     2221 gctacggcgc acgggccgcg ctggtcgccc aggccggcct cagcgtcgtc tccgccctgg
     2281 tcttcctcgc gaccgagcag ccgtggctga tcgtcgccgc ggccatgatg ggcaacctcg
     2341 cggtcggcac cggcgagacc ggccctttcc tcaccctcga gcaggtggcg gtgacgcgcg
     2401 ccactccgcg cgagcgtctg accaccgcgc tgagcttgta caacctcgcg ggctacgccg
     2461 cctccgcgct cggcgccgcg gcggtcggat acgcgggccc ctcgccccgc ctcctgttcg
     2521 cggtgttcct gctcggcggg ctcgttcaga tgggcgccta tgcattgatg cggcccgacc
     2581 ccggccggtc agcggtcggc cgccacacca ccccgatgcc gtcgcgggcg ctcgtccgcc
     2641 gcctggccgc gctcttcgcg ctggactcgc tggcgggcgg cttcgtgctc cagagtctca
     2701 tcgcgtactg gctctacacg cgcttcgaga tgggcctggc cgcgctgggc tggaccttct
     2761 tcggggccca ggtgctctcg ggcctctccc tgctcctggc cgcgaagctc gcgccccgcc
     2821 tcggcctcgt caacaccatg gtcttctccc acctcatctc gaacgtgctg ctgatcgggg
     2881 tcgcgctggc accatccgcc gggctcgcgg tcaccctgct gctggcccgc cacctgctct
     2941 cccagatgga cgtccccacg cggcagagct tcctcatgct ggcggtgcag gatcatgagc
     3001 gcgagggcgc ggccagcctc accaacatga gccgcacact tgcccaatcg gtcagcccgg
     3061 ccctcaccgg ctgggtgatg gcgagcgtgt cgctcgcggc gccgttcttc ctggggggcg
     3121 ggctcaagat cgcctacgac ctgatgctct acgcgaccat tcgccgcctg gagacgcgat
     3181 gacccggctc ctcacccgtg gcgacgtggc ggccctgctg acgctggacg actgcatcgg
     3241 cgcggtcgag caagggtttg cgctccaggg cgccgggcgg gccttcggtc ccggcgtgct
     3301 cgggctccac gtccccgatg gaggctttca cgtcaaggcg gccgggctgc cgctctcgcg
     3361 cctctacgtc gcggtcaagc tcaacgccaa cttccccgcc aacggccgac ggcgcgggct
     3421 acccacgatc cagggcgtcc tcgcgctctc cgacggcgag accggacaac ccctggcgct
     3481 gatggactcc atggaaatca ccgtgctgcg gacgggcgcg gccaccgcgg tggcggcccg
     3541 ccacctcgcg cgacccgacg cgcgcgtggc caccatcgtg ggctgcggac accaggggcg
     3601 catccagctc gccgcgctcg cgcgggtgcg tccgctcgag cgtgcgtacg cggtggacac
     3661 ggacaccacg gtggcgcagc gattcgcgga ggagacgtcc gcccggctcg ccctggacgt
     3721 gcggccggcc gggtcggtcg ccgatgtcgc gccgttgagc gatatctgcg tgacgtgcac
     3781 gccgtcgcgg actcccctcc tgcgtcgcga gcacgtcgcc ggcgggacct tcatcgccgc
     3841 ggtgggcgcc gacaacgagg acaaggtcga gatcgatccg gcgctgatgg cggccgggac
     3901 catcgtggtg gacagcctcg agcagtgcgc gaccatcggc gatctccacc acgcgctcgc
     3961 gctcggcgtc gtctccatgg ctgacgtgca cgcggtactg gccgacgtgg tcgtcggtcg
     4021 caagccgggc cggacatcgg ccgacgagat cacgatcttc gatagcaccg gcgtggcgat
     4081 cgaagacgtg gccgccgcgg ccgtagtcta cgagcgagcc ctcgcgctcg ggcgcggcct
     4141 cacgatcgac ctggcgggct gaggtcggct accgctgaag cactcgccgc atgccccggt
     4201 ccacgaccgc cggcgcgatg acgctcatca ccgccaggat gcgcaccggc cagtagggat
     4261 agacctccgg gcgcggccgg cgcacgcagc ggaggatcga tcgggccacg cgctcggcgg
     4321 actgcaccgg gcccatcggc cgccacggca ccttccggac ctcggcctcg tggaattcgg
     4381 tttcgtcggt gcggatcggg tacacgagcg tcaccgcgat ccccgtcccg gtcagctcca
     4441 cccggagcgc ttcgctgagc gcgcccagcg cgaacttggt cgcgctgtag gccgagcggt
     4501 agggcaccgc gcggcgcccg acgacggacg agatgttgat gacgtgaccg ctcccctgcc
     4561 gccgcatgat gggcagtgcc gcctgggtgg cgccgagcgc ggccatcacg ttcagctcca
     4621 tcagggcgcg cagctccgcg gcggtggtct gctcgagcgt ggccgcgaac ccccgccccg
     4681 cgttgttgac caggacgtcg agccgccccc agcgcgcggc cacctcgtcc accatagcgc
     4741 gcaccgcgag gggatccgtc acgtccaccg cgtgcacggc ggcctcggtc ccccgcgcgg
     4801 tcaattcggc cgcgagctgc tcgagtcgat ccttgcggcg cgcgcacagc gccacgcgca
     4861 gcccggcgcc cgcgaagaca cgcgcggtgg ccgccccgat gcccgatgat gcccccgtga
     4921 cgagcgcgac gcgcccgcgg gggtcaccca cggcgggaac ggcccagcat gcaggagatc
     4981 tggtagcggt gacgcgccac ccagccgtag aggcgcgggg cgagcagctc catgccgggc
     5041 aagcggaagg cctgcgccag ccaccgccag ccgcgcagcc ggcggaggat ctccgggatc
     5101 gccgcgtctc ccgcgagcac acggccatcc ggcaggatga gctgcatggc ctgcaggcac
     5161 gtgccctcgc ccatccacgg gtagcgggcg cggcgctcgg ccgcctggca cggcaggaac
     5221 tcgagatgcc cgctcaccgc gcgccgcgag agccacgaca ccccgccgcg gcagagaccg
     5281 caggtgccgt cgtagatcag gacggcccgg gcccgggatg ccggggcctg gggcacttcg
     5341 agcttggagg tcatcggg
//
LOCUS       DASUFA010000057         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_929808, whole genome shotgun sequence.
ACCESSION   DASUFA010000057 DASUFA010000000
VERSION     DASUFA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929808"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFX87_02185"
     CDS             complement(<1..845)
                     /locus_tag="VFX87_02185"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4993742.1"
                     /translation="MPITVTRLSPRFAARIDGADISRPLDEATWTAIRDAFDEHSVLL
                     FRGAGLDDETQVAFSLRFGALEVTRSMNPAAGTPFARQSNLDIKTGQVIPAEDRRMIY
                     QLANMLWHSDSSFKPVPSLCSLLTGRIVPPEGGATEFASARAAYPSLPEDLQRRADHA
                     VVVHDFSWSRDQIRPGFFTPEERAVYPPVRHRLVRANPVNGRRALFLGAHASHVEGLP
                     VEEGRALLRTLLDHVTQPEFVYRHEWEAGDLVIWDNRCVLHRATPYDTVRYQRLMQRT
                     TIAGDP"
     gene            complement(853..1686)
                     /locus_tag="VFX87_02190"
     CDS             complement(853..1686)
                     /locus_tag="VFX87_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX4993743.1"
                     /translation="MKTPVEKAAAFRALHERPGAFVIPNPWDAGTARLLASLGFEALA
                     TTSLGLANSLGRVDGTNGVSRAQVIENCRVIAAATDLPVNADLENGYAHEPRAAAEII
                     RLAAEVGISGGSIEDATGVADKPIYDFPHAVERVQAAVEVARALPIPFMLTARAENLL
                     HGRLDLDDTIRRLQAFEKAGADVLYAPGVRDLATIRTVVSSVTKPVNVVMSAADPDLT
                     VAQLASVGVKRISVGGALSRLALAAFMKGAREMAERGGFTWMRDTVPSKELKDVFRRW
                     P"
     gene            complement(1701..>2640)
                     /locus_tag="VFX87_02195"
     CDS             complement(1701..>2640)
                     /locus_tag="VFX87_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX4993744.1"
                     /translation="PNNRKQAMLPTTAEVIDNPIGTACGFAVDIGRARFYFTPGVPRE
                     LRRMLEEQIVPRLLAKAGLPGAIFLKRFHSYGLGESHVDSLLAGVEDLVPDGSVKLGF
                     RAHYPQLETKLTARGRDMDDIRRKLEPVEREVRKRVGNFILGEDDQTLEGVILKELQG
                     QAGTLSLVETFTSGQMASRLAHLPGADTVFRRGVIARALPEVWAAVGLAGAPPDGPLT
                     PQTAETVARAARNHTGATHALAVLIDLDDGPDRIEFGGTICLAIATERDVAARKARIL
                     GGREWVRLGAVEMGLDCLRRYLQKLPVYERIDFEKA"
BASE COUNT          350 a          911 c          998 g          381 t
ORIGIN      
        1 ggatcacccg cgatggtggt ccgctgcatg aggcgctggt agcgcacggt gtcgtagggg
       61 gtggcgcggt ggaggacgca gcggttgtcc cagatcacca ggtcgccggc ctcccactcg
      121 tgccggtaga cgaactcggg ctgggtcacg tgatcgagca gggtccgcag cagcgcgcgg
      181 ccttcttcca ccggcaggcc ctccacgtgg gaggcgtgcg cgcccaggaa gagggcgcgg
      241 cggccgttca ccggattggc gcggacgagc cggtggcgca ccggcgggta caccgcgcgc
      301 tcctcggggg tgaagaagcc cggccggatc tggtcgcgcg accacgagaa gtcgtgcacc
      361 acgaccgcgt gatccgcccg tcgctgcagg tcctccggca gcgacgggta ggcggcgcgc
      421 gcgctcgcga actcggtggc gccgccctcg ggcggtacga tgcgcccggt cagcagcgag
      481 cagagcgagg gcaccggctt gaacgagctg tcgctgtgcc agagcatgtt cgcgagctgg
      541 tagatcatcc ggcggtcctc ggcggggatg acctggccgg tcttgatgtc gaggttcgac
      601 tggcgcgcga acggggtgcc cgcggccggg ttcatgctgc gggtgacctc gagtgcgccg
      661 aagcgcaggc tgaaggcgac ctgcgtctcg tcgtcgagcc cggccccgcg gaagagcagc
      721 accgagtgct cgtcgaaggc gtcgcggatg gcggtccacg tcgcctcgtc gagcgggcgg
      781 ctgatgtccg cgccgtcgat ccgcgcggcg aagcgggggc tgaggcgggt caccgtgatc
      841 ggcatcgggg ctctacggcc acctccggaa cacgtccttc agctccttgc tcggcacggt
      901 atcgcgcatc caggtgaagc cgccgcgctc ggccatctcg cgggcgccct tcatgaacgc
      961 ggccagggcc aggcgggaga gcgcgccgcc cacgctgatg cgcttcacgc cgaccgacgc
     1021 gagctgggcc acggtgaggt ccgggtccgc cgcgctcatc accacgttga ccggctttgt
     1081 caccgacgac accacggtgc ggatggtggc gaggtcccgg acgccggggg cgtagagcac
     1141 gtccgcgccc gccttctcga aggcctggag gcgccggatg gtgtcgtcga gatccaggcg
     1201 gccgtgcagg aggttctcgg cccgcgcggt cagcatgaac gggatgggca gggcgcgcgc
     1261 cacctccacc gcggcctgca cccgctcgac cgcgtgcggg aagtcgtaga tgggcttgtc
     1321 ggccacgccg gtggcgtcct cgatggagcc ccccgagatg cccacctcgg ccgcgagccg
     1381 gatgatctcg gcggcggcgc gcggctcgtg ggcgtagccg ttctccagat cggcgttcac
     1441 cggcaggtcg gtggccgccg cgatcacccg gcagttctcg atcacctggg cgcggctcac
     1501 cccgttggtg ccgtccacgc gcccgagcga gttggccagc ccgaggctgg tcgtggcgag
     1561 cgcctcgaag cccagcgagg cgaggagccg cgcggtgccc gcgtcccacg ggttcgggat
     1621 cacgaaggcg ccgggccgct cgtgcagcgc gcggaaggcg gccgccttct ccacgggggt
     1681 tttcatgagt gagactcctc ttacgccttc tcgaagtcga tccgctcgta caccggcagc
     1741 ttctgcaggt accggcgcag gcagtcgagc cccatctcga ccgcgcccag gcgcacccac
     1801 tcgcgcccgc ccaggatgcg ggccttgcgg gcggccacgt cccgctcggt ggcgatcgcg
     1861 aggcagatgg tgcccccgaa ctcgatgcgg tcggggccat cgtcgaggtc gatcagcacc
     1921 gcgagcgcgt gggtggcgcc ggtgtggttc cgagccgcgc gggccaccgt ctccgcggtc
     1981 tgcggcgtga gcgggccgtc gggcggggca cccgcgaggc cgaccgcggc ccacacctcc
     2041 ggcagggcgc gggcgatgac gccccgacgg aagaccgtgt cggcgccggg caggtgggcg
     2101 aggcgcgacg ccatctgccc gctcgtgaac gtctcgacca gcgagagcgt gcccgcctgg
     2161 ccctggagct ccttcaggat gacgccctcg agcgtctgat cgtcctcgcc caggatgaag
     2221 ttgccgacgc gcttgcgcac ctcgcgctcc accggctcga gcttgcggcg gatgtcgtcc
     2281 atgtcccggc cgcgcgcggt cagcttggtc tcgagctgcg ggtagtgggc gcggaagccg
     2341 agcttcacgc tcccgtccgg cacgaggtct tccacacccg ccaggagcga gtccacgtgc
     2401 gattccccga ggccgtagga gtggaagcgc ttgaggaaga tggccccggg caggccggcc
     2461 ttggcgagca ggcgcgggac gatctgctcc tccagcatgc ggcgcagctc gcgcgggacg
     2521 cccggggtga agtagaagcg cgcccggccg atgtccaccg cgaagccgca ggcggtgccg
     2581 atcgggttgt cgatgacctc ggcggtggtc ggcagcatgg cctgcttgcg gttgttgggc
//
LOCUS       DASUFA010000058         9612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_235082, whole genome shotgun sequence.
ACCESSION   DASUFA010000058 DASUFA010000000
VERSION     DASUFA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235082"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFX87_02200"
     CDS             <1..363
                     /locus_tag="VFX87_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4993745.1"
                     /translation="PDGSSSYFLPRMIGLRRALELHYTNRVLSAREAMEWGLVNRVHP
                     DAEFPGAVAALARELAQGPTQAFGRAKLLFHQSTQESLETQMELEAQAIAASGHTEDF
                     RNGVVAFARKQPVTFTGR"
     gene            369..854
                     /locus_tag="VFX87_02205"
     CDS             369..854
                     /locus_tag="VFX87_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993746.1"
                     /translation="MGRLDPGELGRRLDDILAAGERMIRPVPEADLDPPLRALAFGLF
                     RLGLGFADGMDLGRFPEAWRHEPAPADLRDGASVARYGALVRGRLAGWFEGAGPREFA
                     RVIAVHDGPQPGHELLERVTGAAADILRALHDTLTRRGLGPREPLPAALAGLPAPARP
                     W"
     gene            859..4257
                     /locus_tag="VFX87_02210"
     CDS             859..4257
                     /locus_tag="VFX87_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4993747.1"
                     /translation="MTTTPEAGPVPCPRCGAENLPSHRFCVACGQALQATCARCGFVH
                     EPGARFCGGCGQPLLVAGPIRFASPETYTPAHLAEKIRTSRGTLEGERKQVTVLFADM
                     KGSLELLADRDPEDARRMLDPVLRVMMEAVHRYEGTVNQVLGDGVMAIFGAPVAHENH
                     AVRAAYAALRMHEEVHRLAHDLRRAEGVDVQIRVGLNSGEVVVRAIGSDLHMDYSAVG
                     QTTYLAAQMERLARPGATLLAPETRRLTEGFLAVTALGPVPIKGITEPVEVYELTGAG
                     PARYRLQAAAVRGLTRFVGREGEVERVREAFARACAGEGRLVALVGEPGVGKSRVIRE
                     FVEGPWMEGAILLEGRPVAYRKTPSWMPVMDALRRYCQIDRLDEERIIREKITGKLLA
                     LDHALAPALPALLALADLEPEDEAWRALDPPQRRRRIQDAVRNVLLAESRRQPVVLVV
                     EDLQRIDEETQALLDNLVDRLQGTRLLILVAYRPEYQHQWGSKPGYAQIRIDPFPPVT
                     AHALLESLLGSDPGLAALKAVLVERTDGNPLFLEESVRALVETQALIGEPGARRLAKP
                     LENVQVPGSVQAVLAARIDRLTPAAKHVLQSASVIGKDVPFPVLAEIADLPDEGLRAG
                     LALLQSGELLYEASFFPEIEYTFKHALTHEVAYGSLLHETRRALHARIVDAIERLYAG
                     RLGEHVERLAHHALRGGLRERAVDYLRQAGAKAAARSAHREAVGFFEQALEILEADGP
                     RGSARETAIDLVFDLRASLAPLGEFARTLDHLRRAAAQAEALGDRRRLGWVSAYLTQS
                     HYTLGEQAAAIRAAERALEAGRVLRDDALQIVAAFGLGQAHHVLGDYRAAQRHLGRAV
                     SAVDGALSRERWGMAGLISVAARIWLAASLADTGGFAEALAHAREGVALAETPEHPWS
                     LAGAYMTLGFVHLSQGHLDEAGPVLDRGITFSREMDLTAWLPMLLCARGVAHARTGRV
                     ADGLLLLEEGVRRASELRILSRHALRLVWLAEGYLLAGRVDGAADVTDQAQRLAADHA
                     EQGYAAMAVRMAGEVAARAGQGARAAERYTEALARARGLQMRPLEALGHFGLGELARR
                     AGDRAAAKARLTEAVALLREMDMRYWLTQAEAALRDVDERGVER"
     gene            complement(4258..4938)
                     /locus_tag="VFX87_02215"
     CDS             complement(4258..4938)
                     /locus_tag="VFX87_02215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX4993748.1"
                     /translation="MDLVPTDLVEVPAGWFWMGWERGHPGERPRHRVWLDHFAIARAP
                     VTNREYATFLEGGETEPPAWWRDPRFNDADQPVVGVSWFDATGFCRWLAGREGVSYRL
                     PTEAEWEKAARAGLDDARFPWGEERPPTASFDRPPRPTETPANPLGLVGLSGVCHEWC
                     LDWEDEAYYARSPSLNPRGPETGTRRVSRGGAWRHQDAWSPVAHRSSLPPALRYSDYG
                     FRVVHEAG"
     gene            4964..6136
                     /locus_tag="VFX87_02220"
     CDS             4964..6136
                     /locus_tag="VFX87_02220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX4993749.1"
                     /translation="MNFADRMSRLGTESAFEVLARAKALERQGKEIIHLEIGEPDFDT
                     PVHIREAAKRALDSGATHYGPAAGLPELREAIVKDVEGTRSIPVATEEVVVTPGAKPI
                     MYFVITALVNPGDEVVHPNPGFPIYESVINFVGGKPVPIPLREESGFGFDLDVLEKSV
                     SRKTKLIIINSPQNPTGGVLEVEQLGRIAEIAKTYRIPVLSDEIYKSFLYEGEFASIT
                     RFPGMKDLVIILDGFSKSFAMTGWRLGYGVMPVALAEHVTRLMVNSNSCTASFTQMAG
                     IAALQGDQTPVIEMVAEFKRRRDVLVAGLNRLPGVSCRSPRGAFYVFPNIKALKRPST
                     EVAETLLREAGVAVLGGSAFGEYGEGYLRLSYASAEANLRKALERMEPVLARLAGG"
     gene            6149..7792
                     /locus_tag="VFX87_02225"
     CDS             6149..7792
                     /locus_tag="VFX87_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4993750.1"
                     /translation="MPTVAELIVEGLLRAEVPRLFGVPGGGSNLEVLEAARARGLPFV
                     LCHQEWAATIMAAVTGELTGRPGAVLSTLGPGVTASATGLAHARLDRSPLLYISDRHP
                     AGVLAYATHQYVDHAAHLGPIVKGSVTVTPDSAGHWVAHAVQLALAEPRGPVHLDLPA
                     DVAGAAAVPVATAMTPPALPAPDDALVERAAEMIRGAKRPLVIAGLGCRAADAKWLRA
                     FSEALPAPVLTTYKAKGAIPDPHPLAMGIFTGGALEEPLVRRADLIIAFGLDTVELIP
                     RRWSYSAPVLSLARGPSSDARLRAMGGGAYFTPALEVVGDLGAILEDLAPRIMRRDVR
                     ADWDVAEVDHIRRERAAALEVPVAGLAPHRVAQIARELTAAGSIATVDAGAHMFQTTT
                     YWHSLEPGELLISNGLATMGFALPAAIATQLVHPDRRVICFTGDGGLMMVASELETVT
                     RLRLPIVIVVFNDAALSLIEVKQEQKGFEGVSMRYAGPDLLALGRAFGIRALAATDEP
                     TLGSALTAAQTAPGPTLIDARIDPSGYRRMLEIVRGAPK"
     gene            7811..8236
                     /locus_tag="VFX87_02230"
     CDS             7811..8236
                     /locus_tag="VFX87_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993751.1"
                     /translation="MGFLSAKDEAAVRKEFERIGGPVKLTVFASELGGEHNAQAVALI
                     REVAALSDQLSVTVLNPHIDRDEAAAYEVTDSPVVVVEGAQDYGIRFLGIPGGYEFSN
                     LIDSIIAVSTGEVGLSDETKSTLAGLTEDVTIKVFTTPT"
     gene            8249..8467
                     /locus_tag="VFX87_02235"
                     /pseudo
     CDS             8249..8467
                     /locus_tag="VFX87_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            8584..9012
                     /locus_tag="VFX87_02240"
     CDS             8584..9012
                     /locus_tag="VFX87_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX4993752.1"
                     /translation="MRSKRPNAATAASSSELLFTYGTLMRGFRLHRLLTGRAEFLTRG
                     QVRARIIDLGSYPGAVPDPSLRLHGEVYRVAEPALWAVLDSAEGPQYHRREITVRSED
                     GRELAASIYWYIGPLDRGVPIPGGDYRAHAPATSIYHQSL"
     gene            9023..>9612
                     /locus_tag="VFX87_02245"
     CDS             9023..>9612
                     /locus_tag="VFX87_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4993753.1"
                     /translation="MPIEAAQLIKDDQDHLIHPLHHPSDHLEPMVYVRGRGAVLTDVQ
                     GREYIDGLAGLWNVNVGHGRAELADAAAAQMRELAYYSAYSGSTNIPAIQLASKLMTI
                     AYPNMQGVFFTSGGAESNESAFKTARFDWKAKGKPDKVKIIARSLAYHGVTLQAMSAT
                     GMAAYWKMFEPRVPGFVHIPTCYPYRQEGAKPGETAG"
BASE COUNT         1290 a         3475 c         3508 g         1339 t
ORIGIN      
        1 ccggacggct cctcgtccta cttcctgccg cggatgatcg ggctgcgccg cgcgctcgag
       61 ctgcactaca cgaaccgcgt cctctccgcc cgcgaggcga tggagtgggg gctggtcaac
      121 cgcgtgcacc cggacgccga gttccccggc gcggtcgccg cgctcgcccg cgagctggcc
      181 caggggccga cccaggcgtt cggacgcgcg aagctgctct tccaccagtc cacccaggag
      241 agcctggaga cgcagatgga gctggaggcg caggccatcg cggccagcgg ccataccgag
      301 gacttccgca acggcgtggt cgcgttcgcc cgcaagcagc cggtgacgtt cacgggccgc
      361 taggagccgt gggccggctc gatccgggcg agctgggccg ccggctggac gacatcctcg
      421 cggccggcga gcgcatgatc cgccccgtgc cggaggccga cctggatccg ccgctccgcg
      481 ccctcgcctt cgggctcttc cgtctcggcc tgggtttcgc ggacggcatg gacctgggcc
      541 gcttcccgga agcctggcgc cacgagcccg ctcccgccga tctgcgggac ggcgcctcgg
      601 tggcccgcta cggcgcgctg gtgcgcggcc ggctcgcggg ctggttcgag ggcgccgggc
      661 cgcgcgagtt cgcgcgcgtc atcgcggtgc acgacgggcc ccagccgggc cacgagctgc
      721 tcgagcgcgt gaccggcgcg gcggcggaca ttctgcgcgc cctccacgac acgctcacgc
      781 gccgcgggct cggtccgcgc gagccgctgc ccgccgcgct cgcggggctc ccggcgcccg
      841 cccggccctg gtagccgcgt gaccacgacg cccgaggcgg ggccggtgcc ctgcccccgc
      901 tgcggcgccg agaacctgcc gagccatcgc ttttgcgtgg cgtgtggcca ggcgctccag
      961 gccacgtgcg cgcgctgcgg cttcgtgcac gagcccggcg cgcgcttctg cggcggctgc
     1021 ggccagccgc tcctcgtcgc cggcccgatc cgcttcgcct cgcccgagac ctacacgccg
     1081 gcgcacctgg ccgagaagat tcgcacgtcg cgcggcacgc tcgagggcga gcgcaagcag
     1141 gtcacggtgc tgttcgccga catgaagggc tcgctggagc tgctggccga ccgcgatccc
     1201 gaggacgcgc ggcgcatgct cgatccggtg ctgcgcgtga tgatggaggc ggtccaccgc
     1261 tacgagggca ccgtgaacca ggtgctcgga gacggggtca tggccatctt cggcgcgccg
     1321 gtggcccacg agaaccacgc ggtgcgcgcc gcctacgccg cgctgcggat gcacgaggag
     1381 gtgcaccgac tggcgcacga cctgcgccgc gccgagggtg tcgacgtgca gatccgggtc
     1441 gggctcaact ccggcgaggt ggtggtgcgc gccatcggca gcgacctgca catggactac
     1501 agcgcggtgg gccagaccac ctacctggcg gcccagatgg agcgcctcgc gcggcccggc
     1561 gcgacgctgc tcgccccgga gacgcggcgg ctcaccgagg gattcctggc cgtgacggcc
     1621 ctcgggccgg tgccgatcaa gggcatcacg gagccggtcg aggtgtacga gctaaccggg
     1681 gcgggcccgg cgcgctaccg gctgcaggcc gcggcggtgc gcggcttgac gcgcttcgtt
     1741 gggcgcgagg gcgaggtcga gcgggtgcgc gaggccttcg cccgggcctg tgcgggcgag
     1801 gggcggctgg tggcgctggt cggcgagccg ggtgtcggca agtcgcgcgt catccgcgag
     1861 ttcgtcgagg ggccgtggat ggaaggggcg atcctgttgg aaggccggcc ggtcgcatac
     1921 cggaagacgc cgtcgtggat gccggtgatg gatgcgttgc gacgctactg ccagatcgac
     1981 cggctcgacg aggagcggat catccgggag aagatcaccg gcaagctcct ggcgctcgat
     2041 cacgcgctcg cgcccgcgct gccagccctg ctcgcgctcg ccgatctcga gcccgaggac
     2101 gaggcgtggc gcgcgctcga tccgccgcag cgccggcgcc gcatccagga cgcggtgcgc
     2161 aacgtgctcc tggccgagag ccggcgccag ccggtggtct tggtggtcga ggatctccag
     2221 cgcatcgacg aggagaccca ggcgctgctc gacaacctgg tggatcggct gcagggcacg
     2281 cgcctgctga tcctggtcgc ctaccggccg gagtaccagc accagtgggg cagcaagccc
     2341 ggctacgcgc agatccggat cgacccgttt ccgcccgtga cggcccacgc gctcctcgag
     2401 agcctgctcg gctccgatcc cggcctggcc gcgctcaagg cggtgctcgt ggagcggacc
     2461 gacggcaacc cgctcttcct cgaggagagc gtgcgggcgc tcgtcgagac gcaggccctg
     2521 atcggggagc cgggtgcccg ccgcctcgcg aagccgctcg agaacgtgca ggtcccgggc
     2581 agcgtgcagg cggtgctggc cgcccgcatc gaccggctca ccccggcggc caagcacgtg
     2641 ctccagtccg cgtcggtgat cggcaaggac gtgccgttcc cggtgctcgc cgagatcgcc
     2701 gatctgcccg acgaggggct gcgcgccggg ctcgcgctgc tgcagtcggg cgagctgctc
     2761 tacgaggcga gcttcttccc cgagatcgag tacacgttca agcacgccct gactcacgag
     2821 gtggcctacg gcagcctgct ccacgagacg cgccgcgcgc tgcacgcgcg catcgtggac
     2881 gccatcgagc ggctctacgc cggtcgcctg ggcgagcacg tggagcgcct ggcccaccac
     2941 gccctgcgcg gcggcctgcg cgagcgggcg gtggactacc tccgccaggc tggcgccaag
     3001 gcggccgccc gctcggcgca ccgggaagcg gtgggcttct tcgagcaggc gctcgagatc
     3061 ctggaggccg acggcccgcg cggatccgcg cgggagacgg ccatcgatct cgtcttcgac
     3121 ctgcgcgctt cgctcgcgcc gctcggagag ttcgcccgga ccctcgatca tctgcgccgc
     3181 gccgccgcgc aggcggaagc gctcggcgac cggcgacggc tcggctgggt ctcggcctac
     3241 ctcacccagt cgcactacac gctgggcgag caggccgccg ccatccgcgc ggccgagcgc
     3301 gcgctggagg ccggccgggt cctgcgggac gacgcgctcc agatcgtcgc cgccttcggg
     3361 ctcggccagg cccaccacgt gctcggggac tatcgcgcgg cgcagcggca cctcggccgc
     3421 gccgtgtccg cggtggacgg cgcgctgagc cgcgagcggt ggggcatggc gggcctcatc
     3481 tcggtggccg cgcgcatctg gctcgccgcc tcgctggccg acaccggcgg tttcgccgag
     3541 gcgctcgccc acgcgcgcga gggggtggcg ctcgcggaga ccccggagca tccctggagc
     3601 ctggcgggcg catacatgac cctgggtttc gtgcatctga gccagggcca tctggacgag
     3661 gccgggcccg tgctcgaccg ggggatcacg ttctctcggg agatggatct caccgcgtgg
     3721 ctgcccatgc tgctctgtgc gcgtggcgtg gcccacgcgc ggaccgggcg cgtggcggac
     3781 ggcctgctgc tgctcgagga gggcgtgcgc cgcgcgagcg agctgcgcat cctgagccgg
     3841 cacgcgctgc gcctggtctg gctcgccgaa ggctacctgc tggccggccg tgtggatggt
     3901 gccgccgacg tcaccgatca ggcgcaacgg ctcgcggccg accatgccga gcaggggtac
     3961 gcggcgatgg cggtgcggat ggccggagag gtggcggccc gggccgggca gggggcgcgc
     4021 gccgcggagc gctacaccga ggctctggcc cgggcccgcg ggctccagat gcgcccgctg
     4081 gaggcgctcg gccacttcgg gctgggtgag ctcgcgcgtc gggcgggcga ccgcgccgcg
     4141 gcgaaggcgc gactcaccga ggcggtggcc ttgctccgcg agatggacat gcgctactgg
     4201 ctgacccagg ccgaggccgc gctgcgcgac gtcgacgagc gcggggtcga gcgctgatca
     4261 gcccgcctcg tgcaccactc tgaagccgta gtccgagtag cgcagcgccg ggggcagcga
     4321 ggagcgatgc gccaccgggc tccacgcgtc ctggtgccgc caggcgccac cccgtgagac
     4381 gcggcgcgtt ccggtttccg gtcctcgcgg gttcagcgaa ggcgaccgcg cgtagtaggc
     4441 ctcgtcctcc cagtccaggc accattcgtg gcagaccccg gagaggccga cgaggccgag
     4501 cgggttcgcg ggggtctcgg tcggtcgggg cggccggtcg aaggacgccg tcggcggccg
     4561 ctcctcgccc caggggaagc gcgcgtcgtc gaggccggcc cgcgcggcct tctcccactc
     4621 cgcctcggtc gggagccggt aggacacacc ctcgcggccg gccagccacc ggcagaagcc
     4681 cgtcgcgtcg aaccagctca ccccgaccac gggctggtcg gcgtcgttga agcgcgggtc
     4741 ccgccaccag gcgggcggct cggtctcgcc gccttcgagg aaggtggcgt actcgcgatt
     4801 ggtcaccggc gcccgggcga tggcgaagtg atcgagccag acgcgatgcc gcggccgctc
     4861 gcccggatgg ccgcgctccc agcccatcca gaaccagccg gcggggacct ctaccaggtc
     4921 cgtgggcacc aggtccatgg cgtcgagtat aatcggcggc accatgaact tcgcggatcg
     4981 gatgtcgcgg ctcggcacgg aatcggcgtt cgaggtcctg gcccgtgcga aggccctcga
     5041 gcggcagggc aaggagatca tccacctcga gatcggggag ccggacttcg acaccccggt
     5101 ccacatccgc gaggcggcca agcgggcgct cgacagcggg gcgacccact acgggccggc
     5161 cgccgggctc cccgagctcc gggaggccat cgtcaaggac gtggagggca cgcgcagcat
     5221 cccggtcgcc accgaggagg tggtggtgac gccgggggcc aagcccatca tgtacttcgt
     5281 gatcaccgcg ctggtgaatc ccggcgacga ggtggtccac cccaatcccg gcttccccat
     5341 ctacgaatcc gtgatcaact tcgtcggcgg caagcccgtg ccgattcccc tgcgggagga
     5401 gagcggtttc ggcttcgacc tcgacgtgct cgagaagagc gtctcccgca agaccaagct
     5461 gatcatcatc aactcgcccc agaacccgac gggtggcgtg ctggaggtcg agcagctcgg
     5521 acgcatcgcg gagatcgcga agacctatcg catcccggtg ctgtccgacg agatctacaa
     5581 gtccttcctc tacgagggcg agttcgcctc gatcacgcgc ttcccgggga tgaaggacct
     5641 ggtcatcatc ctcgacggct tctcgaagtc gttcgcgatg accggctggc gcctcggcta
     5701 cggcgtgatg ccggtggccc tggccgagca cgtgacccgc ctgatggtga actccaactc
     5761 gtgcaccgcc tcgttcaccc agatggcggg catcgccgcg ctgcagggcg accagacgcc
     5821 ggtcatcgag atggtggccg agttcaagcg gcgtcgcgac gtgctggtgg cggggctcaa
     5881 ccggttgccc ggcgtttcct gccgctcgcc gcgcggggcc ttctacgtgt tcccgaacat
     5941 caaggcgctc aagcggccct cgaccgaggt ggcggagacc ctgctgcggg aggccggggt
     6001 ggccgtgctg ggcggctccg cgttcggaga gtacggggaa ggctacctgc ggctctcgta
     6061 cgccagcgcc gaggccaatc tgcggaaggc cctcgagcgg atggagccgg tgctggcgcg
     6121 gctggccggc ggctagccga tccgctcggt gcccaccgtc gccgagctga tcgtcgaggg
     6181 cctgttgcgg gccgaggtcc cgcgcctctt cggcgtgccg ggcggcgggt ccaacctgga
     6241 agtcctggaa gccgcgcggg ctcgcggcct gcccttcgtc ctctgccacc aggagtgggc
     6301 cgccaccatc atggccgcgg tgaccggcga gctcacgggc cggccgggcg cggtgctctc
     6361 cacgctcggc cccggggtca ccgcgagcgc taccggcctc gcccatgcac ggctcgatcg
     6421 ctcgccgctg ctctacatca gcgatcgcca tccggcaggc gtgctcgctt acgccacgca
     6481 tcagtacgtg gaccacgcgg cgcacctggg cccgatcgtg aagggcagcg tgacggtgac
     6541 gccggactcg gccgggcact gggtggccca cgcggtgcag ctcgcgctcg cggagccgcg
     6601 cggacccgtg cacctggacc tgcccgcgga cgtcgcgggg gcggccgcgg tgccggtggc
     6661 caccgcgatg acgccgccgg cgctgccggc gccggacgac gccctggtcg agcgggccgc
     6721 cgagatgatc cgcggggcga agcggccgct cgtcatcgcc gggctcggct gccgcgccgc
     6781 cgacgccaag tggctgcggg cgttctccga ggcgctaccc gcgccggtgc tcaccaccta
     6841 caaggccaag ggcgcgatcc ccgacccaca cccgctggcg atggggatct tcacgggcgg
     6901 cgcgctggaa gagccgctgg tgcggcgggc cgacctgatc atcgccttcg gcctcgatac
     6961 ggtggagctg atcccgcgcc gctggtccta ctccgcgccg gtgctgagcc tcgcccgcgg
     7021 cccttcgagc gatgcgcgcc tgcgcgcgat gggcgggggc gcgtacttca cgcccgcgct
     7081 cgaggtggtc ggcgacctgg gcgcgatcct cgaggacctg gccccgcgca tcatgcgccg
     7141 ggacgtccgc gcggactggg acgtggccga ggtggaccac atccggcgcg agcgcgcggc
     7201 cgccctcgag gtgccggtgg ccgggctggc tccgcaccgg gtggcccaga tcgcgcgcga
     7261 gctgaccgcg gcgggcagca tcgccacggt ggatgcgggc gcgcacatgt tccagaccac
     7321 gacctactgg cactcgctcg agccggggga gctcctgatc tcgaacgggc tcgccaccat
     7381 gggcttcgcg ctgcccgcgg ccattgcgac ccagctcgtc catcccgacc gacgtgtgat
     7441 ctgcttcacg ggcgacggcg gcctgatgat ggtggcctcc gagctcgaga cggtgacgcg
     7501 cctgcgcctg ccgatcgtga tcgtggtctt caacgacgcc gcgctgtcgc tgatcgaggt
     7561 gaagcaggag cagaagggct tcgagggcgt ctccatgcgc tacgcggggc ccgatctcct
     7621 cgcgctcggg cgcgccttcg gcatccgcgc gctggccgcc accgacgagc cgacgctcgg
     7681 gagtgccttg accgccgcgc agaccgcgcc cggccccacc ctcatcgatg cgcgcatcga
     7741 tccctcgggc taccgtcgca tgctcgaaat cgtccgcggc gccccgaagt aagccgggta
     7801 tactccgggt atgggattcc tctccgcgaa ggacgaggcg gcggtccgca aggagttcga
     7861 gcggatcggc gggccggtga agctcaccgt cttcgcctcc gagctgggcg gcgagcacaa
     7921 tgcccaggcc gtggccctga tccgcgaggt ggcggcgctg tccgaccagc tctcggtgac
     7981 cgtgctcaac ccgcatatcg accgggacga ggccgcggcc tacgaggtca ccgattcgcc
     8041 ggtggtcgtg gtggagggag cgcaggacta cggcatccgc ttcctcggca tcccgggcgg
     8101 ctacgagttc tccaatctga tcgactccat catcgcggtg tccaccggtg aggtggggtt
     8161 gagcgacgag accaagtcca cgctggccgg gctcaccgag gacgtcacga tcaaggtctt
     8221 caccacgccg acctgacccc actgcccccg ggccgtgcgc ctggcgcagc acatggcgat
     8281 cgcctccccg agggtgcggg cggagtgtat cgaggcgacc gagttccccg agctggccca
     8341 gcgctaccag gtgatggccg tccccaagat cgtcatcaac gacaaggtcg gcttcgaggg
     8401 cgcgatcccc gagcgggact tcctgggcgc ggtcctccag gcggtggacc gcgagccgga
     8461 ggcctgagcc catggaatcc gcgctcatcg tcatcggcgt cctgatcggc tggttcgcgc
     8521 tcaaccgctg ggtcctgccg cgcttcggca tccggacctg agcctcgccc gccgcatgct
     8581 cccgtgagga gcaagaggcc gaacgccgcg accgcggcaa gtagctccga gctgctcttc
     8641 acctacggca cgctgatgcg cggcttccgc ctgcaccgcc tgctcacggg gcgggccgag
     8701 ttcctcacgc gcgggcaggt gcgcgcgcgg atcatcgacc tgggctcgta tccgggcgcg
     8761 gtgcccgacc ccagcctccg cctccacggc gaggtctacc gggtcgccga acccgcgttg
     8821 tgggcggtgc tggactcggc ggagggaccc cagtatcatc gccgggagat caccgtccgg
     8881 agcgaggacg gccgcgagct ggccgcctcg atctactggt acatcgggcc gctcgatcgg
     8941 ggcgtgccca ttccgggggg cgactaccgg gcccacgcgc ccgcgacgtc catctatcac
     9001 cagagcttgt aggaggcgcc cgatgccgat cgaggcagcg cagctgatca aggacgacca
     9061 ggaccacctg atccacccgc tgcatcaccc gagcgatcac ctcgagccga tggtctacgt
     9121 gcgcggccgc ggcgccgtgc tgaccgacgt gcagggccgc gagtacatcg acggcctcgc
     9181 cgggctctgg aacgtcaacg ttgggcacgg ccgcgccgag ttggcggacg cggcggcggc
     9241 ccagatgcgc gagctggcgt actactccgc gtactcgggc tcgaccaata tcccggccat
     9301 ccagctcgcc tccaagctca tgacgatcgc gtatcccaac atgcagggcg tgttcttcac
     9361 ctcgggcggt gccgagtcca acgagtccgc gttcaagacc gcgcggttcg actggaaggc
     9421 caagggcaag cccgacaagg tgaagatcat cgcgcgcagc ctcgcctacc acggcgtgac
     9481 gctgcaggcc atgagcgcca ccggcatggc cgcctactgg aagatgttcg agccgcgggt
     9541 gcccgggttc gtgcacatcc cgacctgcta tccctaccgg caggagggtg ccaagccggg
     9601 ggagaccgcg gg
//
LOCUS       DASUFA010000059         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1502485, whole genome shotgun sequence.
ACCESSION   DASUFA010000059 DASUFA010000000
VERSION     DASUFA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502485"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFX87_02250"
     CDS             <1..967
                     /locus_tag="VFX87_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4993754.1"
                     /translation="VDRDQATLLAALPGVARDAGGRSLASAELVVMVDLPRKGESHPN
                     NVPFRGVQRAAFDIRDEARLVEGRRLEPGVREVVVGRKAAGQFAGLSVGSRIGFRDSD
                     WTVVGIFESGGDVHESEIWADAEVAMSAFRRTGYQSVTARLADGSDAGLAAFRDSVGR
                     DRRFSISVLREPEYYAKQATVLSDLINVLGYTVAAFMAVGATFGALNCMYSAIASRQV
                     EIATLRAIGFGGAPVVISVMIEALVLSLLGGAVGGGLAYVYCDGASLSTLNFNTFSQV
                     AFDFRVTPGLLLRGLTWALVIGLAGGLLPAVRAARIPVTEALRTL"
     gene            1059..2759
                     /locus_tag="VFX87_02255"
     CDS             1059..2759
                     /locus_tag="VFX87_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4993755.1"
                     /translation="MHDLVIDNARLIDGLGTPARDGSLAVKDGRIAAISADRHGGDLG
                     AARERVDAGGLVLAPGIVDLHTHYDAQLTWDPFATPSTSLGVTTVVIGNCGFTIAPCR
                     PANRDLVVRNLTHVEGMSLEAMRAGIQWDFESYPEYLASLERRGLVPNVASFVGHSSV
                     RTYVLGEEASKRKATDAEIAEMRRIVVEALRAGAIGFATSTLEQHNGEHGIPMPSRLA
                     DEREMAALTGALGAVGRGVFMLTKGMTSTVPWLEGIAAATRRPVMIAAMFVDPGDPTR
                     VFRELGEIEAARSRGRELWAQVGCFPLGMEFTLRHPYPLEAFIAWRPAIEATEEARYR
                     AVLADPSFRAALKKEIGQPGVPNRFSDKNWDHLTIAEVQRPEHRALEGRTIGTLARER
                     GQHPWDTFLDFGLDGELDAMFDCRLFNIDETEVKRLLRHPHAAVALSDAGAHLSFLCD
                     AGFGLHLFGHWARERGDLTLEQAVRAVTSAVADAYRIQERGRLQAGAWADLMLFDPKT
                     VGRGGKRRVNDLPTGASRLDTPAVGLHGVWVNGVRTATERGVIAGCGRPGRLLREFPG
                     "
     gene            2933..3436
                     /locus_tag="VFX87_02260"
     CDS             2933..3436
                     /locus_tag="VFX87_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993756.1"
                     /translation="MGGVTVATRLERLVRFVPGVAGYQDRENARATDKQVRMRLVGEL
                     QRLIQNLEEDKERIAGSADLSSLPRLDRLAGRLERLGRTVEFAGRGYSGLFDLHTVDG
                     DTLAQLYAFDLGLFDALSAVRAKAEAVRAALPDPEALRSAAEHMREALDDFAELFDQR
                     RRIVDAD"
     gene            3451..4506
                     /locus_tag="VFX87_02265"
     CDS             3451..4506
                     /locus_tag="VFX87_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4993757.1"
                     /translation="MSPIMMEVIEWFDQTGTGIVQRYPEDGSGDIKMGAQLIVRENQA
                     AVFFRDGQGLDVLGPGRHTLSTQNLPILTRLLSLPWGFQSPFRAEVYFVNLKVFTNMR
                     WGTRDPVAFKDNQLGLIRLRAFGSFTMRVTQPLLFVNSLVGTQGSYTTDQIEDYLREV
                     IVARLNDFLGETVQSLLELPRQYDEMAVALKTRLADEFRKYGTEMVDFYINRITPPED
                     VERMIDERSSMSAVGDLDRFLKFKAAKAMGDAAQGRGAGLDGGAAAGVGVGVGAGLGM
                     MMPGMLAKSLGTEPVGGPAATVSCPGCQSAVTADSRFCARCGRQLATATRCARCQAEL
                     PAGARFCSACGQAVPGA"
     gene            4548..>6029
                     /locus_tag="VFX87_02270"
     CDS             4548..>6029
                     /locus_tag="VFX87_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993758.1"
                     /translation="MSQIVVSTECPTCSGPLDFSEGANAIRCPSCGSNLLVTGRKQVL
                     SYWVAPKVKAEVAGAVARTGRLEARVAGARLFFVPFYRLTGHDFQWQDVPPKPEPESP
                     AFPTVGESLGGRESDRPEIEIPIGSMLGWGADVLLGRKAGDTVRDLLGEPPTPERPLD
                     PAVLTAMRATMRPTPTRSAPDGPVQFLDRYVEKTFPAADLAALGVASLGVRAQVLRVS
                     LFERSALAALGTVVAPQLAPERAMAQGLSARGFEHVIYRQVLGRILSVIYFPFWVVEL
                     ARDGERWLTVVDAVAESVVQPRAPLSLREALERPATDEPRTVGLRPLVCPNCGGPLPV
                     EPDDVIFFCGSCHRAWQIHGAELGDMPHEIADVRPDTANPGPAAGAVYLPFWQLDPAG
                     STGAPRAWAPAFRYRRLKALHDLAARLTAKPPAYRPWTGERPAAHGCFYDAEDAALLA
                     RFAAAGRHRTPPEVKAAAEAEPAFSGACLTWLPFRRESRSLLDP"
BASE COUNT          794 a         2225 c         2119 g          891 t
ORIGIN      
        1 cgtggaccgc gaccaggcca cgctgctcgc cgccctgccg ggcgtggccc gcgatgccgg
       61 cgggcgctcg ctcgcctccg cggagctggt ggtgatggtg gacctgccgc gcaaggggga
      121 gagccatccg aacaacgtgc cgtttcgcgg cgtgcagcgc gccgcgttcg acatccgcga
      181 cgaggcgcgg ctcgtggagg gccgccgtct cgagccgggc gtccgcgagg tcgtggtggg
      241 ccgcaaggcg gccggccagt tcgcgggcct ctcggtcggc tcgcgcatcg gcttccgcga
      301 cagcgactgg accgtggtcg gcatcttcga gagcggcggc gacgtgcacg agtcggagat
      361 ctgggccgac gcggaggtgg ccatgtccgc cttccggcgc accggctatc agtcggtgac
      421 ggcccggctc gccgacggct cggacgcggg cctcgcggcc ttccgcgact cggtcggccg
      481 cgatcgccgt ttctcgatca gcgtgctgcg cgagcccgag tactacgcga agcaggccac
      541 cgtgctgtcc gatctcatca acgtgctggg ctacacggtg gccgccttca tggcggtggg
      601 cgccactttc ggcgcgctca actgcatgta ctcggccatc gcgagccgcc aggtggagat
      661 cgccaccctc cgcgccatcg gcttcggcgg cgcgccggtg gtgatctcgg tgatgatcga
      721 ggccctcgtg ctgtcgctgc tgggcggggc ggtgggcggc gggttggcct acgtctactg
      781 tgacggcgcc tcgctctcca cactcaactt caacaccttc tcgcaggtgg ccttcgactt
      841 ccgcgtgacc ccgggcctgc tcctccgcgg gctcacctgg gccctggtca tcggcctggc
      901 cggcgggctc ctgcccgcgg tgcgcgcggc ccgcatcccg gtcaccgagg cgctccggac
      961 gctctgaacc accgcgaccc ggtccgcggt tgactcactt ccggccccgg gctagcctca
     1021 ggcggtcagg caagccaaag aaccgaggag gcctctccgt gcacgatctc gtcatcgaca
     1081 acgcgcgtct catcgacggt ctcggcacgc ccgcgcgtga cggcagcctg gccgtcaagg
     1141 acgggcgtat cgccgcgatc agcgccgatc gccacggcgg cgatctcggc gccgcgcgcg
     1201 agcgggtgga cgcgggcggc ctcgtgctgg cgcccggtat cgtggacctg cacacccact
     1261 acgacgccca gctcacctgg gatcccttcg ccacgccctc gacctcgctc ggggtgacca
     1321 cggtggtgat cggcaactgc gggttcacga tcgccccgtg ccgcccggcc aaccgcgatc
     1381 tcgtcgtgcg caatctcacc cacgtcgagg gcatgtcgct cgaggccatg cgcgcgggca
     1441 tccagtggga cttcgagagc tatcccgagt acctggcctc gctcgagcgc cgcggcctcg
     1501 tgcccaacgt ggcctcgttc gtgggccatt cctcggtgcg cacctacgtg ctcggcgagg
     1561 aggccagcaa gcgcaaggcc accgacgcgg agatcgcgga gatgcggcgg atcgtggtgg
     1621 aggcgctccg ggccggcgcg atcggcttcg ccacctccac actcgagcag cacaacggcg
     1681 agcacggcat cccgatgccc tcgcgattgg ccgacgagcg cgagatggcc gcgctcaccg
     1741 gcgcgctcgg cgcggtcggt cgcggcgtgt tcatgctcac caagggcatg acctccacgg
     1801 tgccgtggct cgagggcatc gcggcggcca cccgccggcc ggtgatgatc gcggccatgt
     1861 tcgtggaccc gggcgatccg acccgcgtct tccgcgagct gggcgagatc gaggccgcgc
     1921 ggagccgggg gcgcgagctg tgggcgcagg tgggatgctt cccgctcggc atggagttca
     1981 ccctccgcca cccctacccg ctcgaggcct tcatcgcctg gcggcccgcc atcgaggcca
     2041 ccgaggaggc gcgctaccgc gcggtgctgg ccgacccgtc gttccgcgcc gcgctgaaga
     2101 aggaaatcgg ccagccgggc gtgcccaacc gcttcagcga caagaactgg gatcacctga
     2161 cgatcgcgga ggtacagcgc cccgagcacc gggctctcga gggccggacc atcggcacgt
     2221 tggcccgcga gcgcggccag catccctggg acaccttcct cgacttcggc ctcgacggcg
     2281 agctcgacgc gatgttcgac tgccgcctct tcaacatcga cgagaccgag gtgaagcggc
     2341 tgctgcgcca cccccacgcc gcggtggccc tgtccgacgc gggcgcccat ctctccttcc
     2401 tctgcgacgc gggcttcggc ctgcacctgt tcgggcactg ggcgcgcgag cgcggcgatc
     2461 tcaccctcga gcaggcggtg cgggcggtga ccagcgcggt ggccgatgcc taccgtatcc
     2521 aggagcgcgg ccggctccag gccggcgcct gggccgacct catgctgttc gacccgaaga
     2581 cagtcggccg gggcggcaag cgccgcgtga acgacctgcc caccggggcc agccggctcg
     2641 acacgccggc cgtcggcctc cacggggtgt gggtcaacgg ggtgcggacc gcgaccgagc
     2701 gcggcgtgat cgccgggtgc ggccgccccg gccgactcct gcgggagttc cccggttagt
     2761 cctgagagtc gccaagaagt gtcagcatac tgacacgccc tgccagaggt tccgtttcac
     2821 tcccctcttc tttcgttcct ttccaatagc ttaagctgat atcgcactgg caatccgctt
     2881 gcacactcct ccgctggtcc gggctcgccc ggagagacga gaggaggcag gcatgggtgg
     2941 agtcaccgtc gcgacacggc tggagcggct ggtccggttc gttccagggg tcgcgggcta
     3001 tcaggaccgg gagaatgctc gagccaccga caagcaggtc cggatgcggc tcgtcggcga
     3061 gctgcagcgg ctcatccaga acctcgagga ggacaaggag cgcatcgccg gctccgccga
     3121 tctctcctcg ctgccgcgcc tcgaccgcct cgcgggacgg ctcgagcgcc tcggccgcac
     3181 cgtggagttc gcgggccgcg gctactcggg cctcttcgac ctccacacgg tggacgggga
     3241 cacgctggcc cagctctacg ccttcgacct cggactcttc gacgcgctga gcgcggtgcg
     3301 ggccaaggca gaagcggtgc gcgccgcgct tcccgacccg gaggcgctcc ggagcgcggc
     3361 cgagcacatg cgggaagcgc tggacgactt cgcggagctc ttcgaccagc gtcggcgcat
     3421 cgtcgacgcg gactaggacg gaccgacgcc atgtcaccga tcatgatgga agtcatcgag
     3481 tggttcgatc agaccggcac cggcatcgtc cagcgctacc ccgaggacgg ctcgggcgac
     3541 atcaagatgg gcgcgcagct catcgtccgc gagaaccagg ccgcggtgtt cttccgcgac
     3601 ggccagggac tcgacgtgct cgggcccggg cgccacacgc tgtccaccca gaaccttccg
     3661 atcctgaccc ggctcctctc gctgccgtgg ggcttccaga gtccgttccg cgccgaggtc
     3721 tacttcgtca acctgaaggt cttcaccaac atgcgctggg gcacgcgtga tccggtggcc
     3781 ttcaaggaca atcagctcgg cctgatccgc ctgcgcgcct tcggcagctt cacgatgcgg
     3841 gtgacccagc cgctcctctt cgtcaacagc ctggtgggca cccagggcag ctacaccacg
     3901 gatcagatcg aggactacct tcgagaagtc atcgtggccc ggctgaacga cttcctcggc
     3961 gagacggtcc agagcctgct cgagctgccg cgccagtacg acgagatggc ggtggcgctc
     4021 aagacacgcc tggccgacga gttccgcaag tacgggaccg agatggtgga cttctacatc
     4081 aaccgcatca cgccgcccga ggacgtggag cggatgatcg acgagcgctc gagcatgagc
     4141 gcggtcggcg atctcgaccg cttcctcaag ttcaaggcgg ccaaggcgat gggcgacgcc
     4201 gcgcaggggc gcggggcggg actggacggc ggcgccgccg ccggggtggg cgtcggcgtc
     4261 ggcgcggggc tgggcatgat gatgccgggc atgctcgcga agagcctcgg caccgagccg
     4321 gtgggcggcc cggcggcgac cgtgagctgc ccgggctgcc agtcggcggt cacggcggac
     4381 agccgcttct gcgcgcgctg cggtcgccag ctcgcgacgg ccacccgctg cgcgcggtgc
     4441 caggccgagc tgccggccgg cgcgcgcttc tgctcggcct gcggccaggc ggtacccggc
     4501 gcctagcgcc ggcgctccgc gccgcgcgcc cctccgacga cgtctccatg agccagatcg
     4561 tcgtctcgac cgagtgtcct acctgcagcg gcccgctcga tttctccgag ggggccaacg
     4621 ccatccgctg tccgagctgc ggctcgaacc tcctcgtcac cggccgcaag caggtcctga
     4681 gctactgggt cgcgccgaag gtgaaggccg aggtcgcggg cgccgtcgcc cgcaccggcc
     4741 gcctcgaggc gcgtgtggcg ggcgcccggc tcttcttcgt gcccttctac cgcctgaccg
     4801 gccacgactt ccagtggcag gacgtgccgc ccaagcccga gccggagagc ccggccttcc
     4861 ccacggtcgg cgagagcctg ggcggccgcg agtccgaccg gcccgagatc gagatcccca
     4921 tcggctcgat gctgggctgg ggcgccgacg tgctgctcgg ccgcaaggcc ggcgacaccg
     4981 tgcgcgatct gctgggcgag cccccgacgc cggagcgtcc gctcgatccg gccgtgctga
     5041 ccgccatgcg cgcgactatg cgccccaccc ccacgcggtc cgctcccgac gggccggttc
     5101 agttcctcga tcgctacgtg gagaagacct tcccggccgc ggacctcgcg gcgctcgggg
     5161 tggcttcgct cggcgtacgc gcccaggtcc tccgcgtgtc gctcttcgag cgctcggccc
     5221 tggccgcgct gggcaccgtc gtcgcgccgc agctcgcgcc ggagcgcgcg atggcgcagg
     5281 ggctgtccgc ccgcggcttc gagcacgtga tctaccggca ggtcctcggg cgcatcctga
     5341 gcgtgatcta cttccccttc tgggtggtcg agctcgcgcg ggatggtgag cgctggctca
     5401 ccgtggtgga tgccgtggcc gagagcgtcg tgcagccccg cgcgccgctg tcgctccgcg
     5461 aggccctcga gcgccccgcg accgacgagc cgcggacggt ggggctccgc ccgctggtct
     5521 gccccaactg cggcgggccc ctacccgtcg agcccgacga cgtgatcttc ttctgcggct
     5581 cctgtcaccg ggcctggcag atccacggcg cggagctcgg cgacatgccg cacgagatcg
     5641 ccgacgtgcg gcccgacacg gcgaatcccg gcccggcggc gggcgcggtc tacctgccgt
     5701 tctggcagct cgatcccgcg ggctcgaccg gcgcgccgag ggcctgggcg cccgccttcc
     5761 gctatcgccg gctcaaggcg ctgcacgacc tggccgcgcg gctgaccgcg aagccgcccg
     5821 cgtaccggcc gtggaccggg gagcgtcccg ccgcgcacgg ctgcttctac gacgccgagg
     5881 acgccgcgct gctcgcgcgc ttcgcggcgg cgggccggca ccggacgccc cccgaggtga
     5941 aggcggccgc ggaggccgag cccgcgttca gcggggcgtg cctcacgtgg cttccattcc
     6001 ggcgcgagtc ccggtcgctg ctcgatccg
//
LOCUS       DASUFA010000060         4139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_471941, whole genome shotgun sequence.
ACCESSION   DASUFA010000060 DASUFA010000000
VERSION     DASUFA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471941"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFX87_02275"
     CDS             complement(<1..292)
                     /locus_tag="VFX87_02275"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX4993759.1"
                     /translation="MPNIVVVGAQWGDEGKGKIVDVLAPHVGVVVRYQGGNNAGHTVV
                     VGREKFVLQSIPSGILHPGVRCVIGCGVVIDPASLIEEMESLVQRGVSLDGNL"
     gene            complement(285..1634)
                     /gene="hisZ"
                     /locus_tag="VFX87_02280"
     CDS             complement(285..1634)
                     /gene="hisZ"
                     /locus_tag="VFX87_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX4993760.1"
                     /translation="MSAPRKSPPTQLPPRQLPKGAKIYLPDEAARKRAVEERLFEVFR
                     RWGYREVVTSAYEYFDVLSHGTDGELQERMFKMVDRESGRLLALRADVTPQIARIVAT
                     RMRDEPKPLRLAYVTDVFRYDEPQVGRYREFYQAGVELIGLPNPEGDAEMIAMTVEGL
                     RALGLERFQIDVGQTEFFRGILEDVAADPATGRELRSALGHKDLSALERLVGEMAAPA
                     AVTELLLALPTLYGRGDVLERAERLVKNPRSEAALANLAEVYRLLRAYGLADSVLLDL
                     GEVRGFDYYSGVHFEAYVSGLGAPLVGGGRYDQMLARFGYDCPATGFAFETGRALLAM
                     ASQGAEPRLPGPDFFVIDFTADKIRALALSRRLRDAGAAVARDIISRPLAESLVYARG
                     QHAAWALIIGTEGADPDLVRILDLRAGAEAAVERMVTCAELLADPRPHFPGLGEGHA"
     gene            complement(1642..2259)
                     /locus_tag="VFX87_02285"
     CDS             complement(1642..2259)
                     /locus_tag="VFX87_02285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4993761.1"
                     /translation="MRVLLIRHAETDWNRERRFQGWRDSPLSAVGRGQAESAARLLAG
                     SPVAAVWSSPLGRARETAAIIAAPHKLAVREADAFKEMGFGEWEGLTRDEVAARFPEP
                     LRAWAETPHEAAWPGAETLAAVRARALAGLEAMRQAHPEQTVCLVSHGITCRVLILEA
                     LGLGLDRLWSLQLASTGISELEFRDDWTTLHRMNSLIHLTDVAIA"
     gene            complement(2265..3404)
                     /locus_tag="VFX87_02290"
     CDS             complement(2265..3404)
                     /locus_tag="VFX87_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX4993762.1"
                     /translation="MKKYYLMAPGPTPVPSNVLLAMAQPMLHHRTPEYEALFLEVRAG
                     LKRLFQTSQDVIPFTSSGTGAMEAAVCNTLSAGDTVLVLRAGKFGERWEEICRTYGVT
                     VIPVDAPFGHTVPASAFVEALHQHPDAKAVLMQHSESSTGVLHDVKGVAAATHETGAI
                     LVVDAVSSLGIADLRMDEWGVDVVVSGSQKGLMLPPGLSFCALSEKAWGRVKTSRLPK
                     YYFDIATERKFVARNECRFTPAVSIFVGLREVLKMIETEGLPNVFRRHERLARATRSG
                     VEALGLELFAKSGPSPALTAVVAPRGIDSEAVLSTYSTSHNITIAGGQGEMKGRVFRL
                     GHMGYVGDFDVITALAALEQVLHELGHPVDFGAAVRAAQKVLAER"
     gene            3506..4069
                     /locus_tag="VFX87_02295"
     CDS             3506..4069
                     /locus_tag="VFX87_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4993763.1"
                     /translation="MPHLAMALLLLATPALAGPARADEGLWQRLAAGGQVVLLRHGTT
                     TPGVGDPTGFRLDNCATQRNLTEAGREESRRIGAALKARAIPVERVLSSPWCRCLETA
                     RLAFGSAEPWEPLSSLFADRTRQAEQTRAFRTLAGAKRAGGNTILVTHGANIVTFVGV
                     SPAMAEMVIVTPQGNGTFTVAGRLPAP"
BASE COUNT          622 a         1542 c         1409 g          566 t
ORIGIN      
        1 agaggttgcc gtccagcgag acgccgcgct gcaccagcga ctccatctcc tcgatcaggg
       61 aggccgggtc gatcaccacc ccgcagccga tcacgcagcg gacacccgga tgcaggattc
      121 ccgaggggat ggactggagc acgaacttct cccgacccac caccaccgtg tggcccgcat
      181 tgttcccgcc ctggtagcgg acgaccacgc ccacgtgcgg cgcgagcacg tccacgatct
      241 tgcccttgcc ctcgtcgccc cactgcgcgc ccaccaccac gatgttaggc atgtccttct
      301 ccaagtcccg ggaagtgcgg ccgcgggtcc gcgagcagct cagcacacgt caccatccgc
      361 tccaccgccg cttccgcgcc cgcccgcaga tccaggatcc gcaccaggtc cgggtcggcc
      421 ccttcggtcc cgatgatcag cgcccaggcg gcgtgctgcc cccgcgcgta gaccagcgat
      481 tcggccagcg gccggctgat gatgtcgcgc gccaccgccg cccccgcatc ccgcagccgc
      541 cgcgagagcg cgagcgcgcg gatcttgtcc gccgtgaagt cgatcacgaa gaagtccggc
      601 ccgggcagcc gcggctccgc gccctgcgag gccatcgcca gcagcgcccg ccccgtctcg
      661 aaggcgaagc cggtggccgg acaatcgtag ccgaaccggg ccagcatctg gtcgtagcgc
      721 ccgcccccca ccagcggggc gcccaggccc gacacgtaag cttcgaagtg tacgcccgag
      781 tagtagtcga agccacgcac ttcgcccagg tcgagcagga ccgagtcggc aagcccgtag
      841 gcgcgcagca gccggtagac ctcggccagg ttggcgaggg cggcctccga ccgcgggttc
      901 ttgacgagcc gctcggcgcg ctcgagcacg tcgccgcgcc cgtagagcgt cggcagggcc
      961 agcagcagct cggtcaccgc cgcgggcgcg gccatctccc ccaccaggcg ctcgagggcc
     1021 gagaggtcct tgtggcccag cgccgaccgc agctcccgcc cggtggcggg gtcggcggcc
     1081 acgtcctcca ggatgccgcg gaagaactcg gtctgcccca cgtcgatctg gaagcgctcg
     1141 agccccagcg cgcggagacc ctcgacggtc atcgcgatca tctcggcgtc gccctcggga
     1201 ttcggcagcc cgatcagctc cacgccggcc tggtagaact cacggtagcg gcccacctgt
     1261 ggctcgtcgt agcggaacac gtcggtgacg taggccaggc gcagcggctt gggctcgtcc
     1321 cgcatccggg tggcgacgat tcgcgcgatc tgcggtgtga cgtccgcgcg cagggcgagg
     1381 aggcgcccgc tctcccggtc caccatcttg aacatgcgct cctgcagctc gccgtcggtg
     1441 ccgtgggaga gcacgtcgaa gtactcgtag gcgctggtga ccacctcgcg atagccccag
     1501 cgccggaaca cctcgaagag gcgctcctcc accgcgcgct tgcgcgccgc ctcgtcgggc
     1561 aggtagatct ttgcgccctt cggaagctgt ctcggcggca gttgagtcgg cgggctcttg
     1621 cgcggcgcgc tcacggcggc ctcaggcgat cgcgacgtcg gtcaggtgga tgagcgagtt
     1681 catccggtgc agcgtggtcc agtcgtcgcg gaactcgagc tccgagatgc cggtggaggc
     1741 gagctggagc gaccagagcc ggtcgaggcc gagcccgagg gcctccagga tcaggacccg
     1801 gcacgtgatc ccgtgcgaga ccaggcagac ggtctgctcc gggtgggcct gccgcatcgc
     1861 ctcgaggccc gccagggccc gggcacgcac ggccgcgagc gtctccgccc cgggccacgc
     1921 ggcctcgtgc ggtgtctcgg cccaggcacg gagcggctcg gggaagcgcg ccgccacctc
     1981 gtcccgcgtg agcccctccc actccccgaa gcccatctcc ttgaaggcgt ccgcctcgcg
     2041 taccgcgagc ttgtgcggag ccgcgatgat ggcggcggtc tcccgcgcgc gcccgagcgg
     2101 gctcgaccag accgcggcga ccggcgagcc ggcgaggagc cgcgccgccg actcggcctg
     2161 cccccggccg accgcggaga gcggactatc gcgccagccc tggaagcggc gctcccggtt
     2221 ccagtcggtc tccgcgtgac ggatcagcag cacgcgcatc gcgctcagcg ctcggccagg
     2281 accttctggg ccgcgcggac cgcagcgccg aagtcgacgg ggtgtccgag ctcgtgcagc
     2341 acctgctcga gcgcggcgag ggcggtgatc acgtcgaagt cgccgacgta acccatgtga
     2401 ccgagccgga acacgcgccc cttcatctcc ccctgcccgc ccgcgatcgt gatgttgtgc
     2461 gacgtcgagt aggtggacag caccgcctcg ctgtcgatcc cgcgcggggc gaccaccgcg
     2521 gtcagcgccg ggctcggccc ggacttggcg aagagctcga gtcccagggc ctccacaccg
     2581 gagcgggtgg cgcgggccag ccgctcgtgg cgccggaaca cgttcggcag gccctcggtc
     2641 tcgatcatct tcagcacctc gcgcagcccc acgaagatgg agacggccgg ggtgaagcgg
     2701 cattcgttcc tcgccacgaa tttccgctcg gtcgcgatgt cgaagtagta cttcggcagc
     2761 cgcgaggtct tcacgcggcc ccacgccttc tccgagagcg cgcagaagga gagacccggc
     2821 gggagcatca gccccttctg ggagcccgag accacgacgt ccacgcccca ctcgtccatg
     2881 cggagatcgg cgatgccgag gctcgacacc gcgtccacga ccaggatcgc accggtctcg
     2941 tgcgttgccg ccgcgacgcc cttgacgtcg tggagcacgc cggtggacga ctcgctgtgc
     3001 tgcatcagca ccgccttcgc gtccgggtgc tggtggagcg cctccacgaa ggcggaggcc
     3061 ggcacggtat ggccgaacgg cgcgtccacc gggatcacgg tgacgccgta ggtgcggcag
     3121 atctcttccc agcgctcgcc gaacttgccc gcgcggagca ccagcaccgt gtcacccgcc
     3181 gacagcgtgt tgcacacggc ggcttccatg gccccggtgc ccgacgatgt gaaggggatc
     3241 acatcctggc tggtctggaa gaggcgcttg agccccgccc ggacctccag gaagagcgcc
     3301 tcgtactccg gggtccggtg gtggagcatc ggctgggcca tcgccagcag gacgttcgaa
     3361 ggcacgggcg tgggccccgg cgccatcagg tagtacttct tcattacgag ctccttgggg
     3421 gtcaggttga cccgaccacg gtatcagaac cgcgcgacac cttgcaacga gcgcccggct
     3481 tgtgaagccg tgtaccatgt ggcccgtgcc ccatctggcc atggccctgc tcctcctggc
     3541 caccccggcg ctcgccggcc ctgcgcgcgc cgacgagggc ctctggcagc ggctcgcggc
     3601 gggaggccag gtcgtgctcc ttcgccatgg cacgacgacc cccggggtcg gggatccaac
     3661 cggctttcgc ctggacaact gtgcgactca gcgcaacctg accgaggccg gacgcgagga
     3721 gtcgcggcgg atcggcgccg ccctcaaggc gcgcgcgatc ccggtcgagc gggtgctgtc
     3781 gagcccatgg tgccgctgcc tcgagaccgc acggctggcc ttcggatcgg ccgagccgtg
     3841 ggaacccttg agctcgctct tcgccgaccg gacacggcag gcggagcaga cgcgtgcgtt
     3901 ccggaccctc gcgggcgcga agcgcgcggg cggcaatacg atcctggtga cccacggcgc
     3961 caacatcgtg accttcgtcg gcgtgagccc cgccatggcc gagatggtga tcgtcacgcc
     4021 gcagggcaac gggaccttca ccgtcgcggg ccgcctcccc gccccgtgac cacggcccgg
     4081 cacgacccga acacggcaac gacacaacta cggaaagaga gctagtccga gcagggcga
//
LOCUS       DASUFA010000061         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_128492, whole genome shotgun sequence.
ACCESSION   DASUFA010000061 DASUFA010000000
VERSION     DASUFA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128492"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFX87_02300"
     CDS             complement(<1..863)
                     /locus_tag="VFX87_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993764.1"
                     /translation="MDEQALREMLAQVQHGGLTRRRFVQALAAAGLGGPVAGHLLGAA
                     GAATAQPREAEFTPTRRGGGGTLRILMWDAPTLLHPHFGRGLRDFTVQRIFYEPLAAP
                     TADGTFVPVLAEELPSRKAGTVAKDGQWVTWRLKRNVRWHDGTPFTADDVIFNWEFAT
                     DPATAAGTRAAYEDISRIERIDSHTVKLFFKKPQPSWDSAFTGGGLLPRHVFDRVKGA
                     GAREAIGMIPAVGTGPYRLAEFRPGDFIRAEINKTYHVPNRPFFDQLEIKCGGDAVGA
                     VRAVMQTGEYD"
     gene            994..2064
                     /locus_tag="VFX87_02305"
     CDS             994..2064
                     /locus_tag="VFX87_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993765.1"
                     /translation="MNLISERLDVDDYDEAVELFYQRNWTDGLPIVLPTRRRVEAMIA
                     GAGRDPQESLGPVPPKGGPATVEKLAINAVMGGCLPAHFPVVLAALEALLDPAHNLNG
                     VSQTTHMCVPLVIVNGPIARELGFNSRDGVFGNGYRANAAVGRAVRLALWNLGGAVPW
                     DTDKATLSHPGEYAFCIAEEEEDNPWAPLHVERGCPPGSDAVTVFACEGPHSVFCYGT
                     PEEMLHVLTDSMCGLGNNNMHVMGQMLVVLNPLNAEEFARRGWSKTDVRRYLWEHARR
                     PIEQVRACGVVHEQFRKMERDAGRYPLRYDLDNPTTRIPVSAEPDDIHIVVAGGRSYF
                     AAVLPGWGGFGGFAVTRAIRRA"
     gene            2068..>2259
                     /locus_tag="VFX87_02310"
     CDS             2068..>2259
                     /locus_tag="VFX87_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993766.1"
                     /translation="MEILSPVWPAPGAGGSALARPRTLAGATIALVDDNLDIPFTTHL
                     EMLLGREHGAAVRRLVKPSG"
BASE COUNT          327 a          782 c          834 g          316 t
ORIGIN      
        1 aagtcgtact ccccggtctg catcaccgcg cgaaccgccc ccaccgcgtc gccgccgcac
       61 ttgatctcga gctggtcgaa gaacggacga ttcggcacgt ggtaggtctt gttgatctcc
      121 gcgcggatga agtcgcccgg ccggaactcc gcgagccggt agggcccggt gcccaccgcg
      181 ggaatcatgc cgatggcctc gcgggcgccg gcccccttga cccggtcgaa gacgtgccgt
      241 gggagcagcc cgccgcccgt gaatgcggag tcccaagaag gctggggctt cttgaagaaa
      301 agcttgacgg tgtgggagtc gatccgctcg attcgcgaga tatcctcgta ggccgcgcgg
      361 gtgcccgcgg cggtggcggg atccgtggcg aactcccagt tgaagatcac gtcgtcggcg
      421 gtgaaggggg tgccgtcgtg ccagcgcacg ttgcgcttga gccgccacgt cacccactgg
      481 ccgtccttgg ccaccgtgcc cgccttgcgc gagggcaatt cctcggccag gaccggcacg
      541 aaggtgccgt cggcggtggg agcggcgagc ggctcgtaga agatccgctg gaccgtgaag
      601 tcgcgcaggc cccggccgaa gtgcgggtgc agcagggtgg gcgcgtccca catcaggatg
      661 cggagggtgc cgccgccgcc gcgccgggtg ggcgtgaact cggcctcgcg cggctgcgcg
      721 gtggccgcgc ccgccgcgcc gaggaggtgg ccggcgaccg gcccgccgag cccggcggcg
      781 gcgagcgcct gcacgaagcg ccggcgcgtg agaccgccgt gctgcacctg cgcgagcatc
      841 tcccgaaggg cctgttcgtc catggatccc ggtgggggtc cgacaccgat aacgcggggc
      901 gagggggaag tcaagggccc aagtcgcttg tcgcgccggg tggaatcggg gacaatacgc
      961 cgtcgcccta ccggcccagg agaccgatcg cccatgaacc tgatctcgga gcgcctcgac
     1021 gtcgacgact acgacgaggc ggtggagctc ttctaccagc gaaactggac ggacggcctc
     1081 ccgatcgtgc tgcccacccg gcggcgcgtg gaggcgatga tcgcaggcgc gggccgcgac
     1141 ccgcaggaga gtctcggccc ggtgccgccc aagggcggcc ccgcgaccgt cgagaagctg
     1201 gccatcaacg cggtgatggg cggctgcctg cccgcgcact tcccggtggt gctggccgcg
     1261 ctggaggcgc tgctcgaccc ggcccacaat ctcaacgggg tgagccagac cacccacatg
     1321 tgcgtccccc tcgtcatcgt caacggtccc atcgcgcgcg agctcgggtt caactcgcgc
     1381 gacggggtct tcggcaacgg ctatcgggcc aacgcggcgg tggggcgcgc ggtgcgcctg
     1441 gcgctgtgga acctgggcgg cgcggtgccg tgggacaccg acaaggccac gctctcccac
     1501 ccgggggagt acgcgttctg catcgcggag gaagaggaag acaatccgtg ggcgccgctc
     1561 cacgtggagc gcggctgccc gcccgggtcc gacgcggtga ccgtgttcgc gtgcgagggg
     1621 ccgcacagcg tcttctgcta cggcacgccg gaggagatgc tccacgtgct caccgactcc
     1681 atgtgtgggc tgggcaacaa caacatgcac gtgatggggc agatgctggt ggtgctgaac
     1741 ccgctgaacg ccgaggagtt cgcccggcgc ggctggtcca agaccgacgt ccgccgctat
     1801 ctctgggagc acgcgcgccg gccgatcgag caggtgcggg cgtgcggagt ggtgcacgag
     1861 cagttccgga agatggagcg cgacgccggc cgttacccgc tccgctacga cctcgacaat
     1921 ccgaccaccc gcatcccggt ctccgcggag cccgacgaca tccacatcgt ggtggcgggc
     1981 ggccgcagct acttcgcggc ggtgctgccc ggctggggcg gcttcggggg cttcgcggtt
     2041 acccgtgcga tccggagggc ctgagccgtg gagatcctga gcccggtctg gcccgcgccc
     2101 ggcgccgggg gatccgccct ggcgcggccg cgcacgctcg cgggcgccac catcgccctg
     2161 gtcgacgaca atctcgacat cccgttcacc actcacctcg agatgctgct gggccgcgag
     2221 cacggggccg cggtgcggcg gctggtgaag ccgtccggc
//
LOCUS       DASUFA010000062         8132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_235417, whole genome shotgun sequence.
ACCESSION   DASUFA010000062 DASUFA010000000
VERSION     DASUFA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235417"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFX87_02315"
     CDS             <1..1021
                     /locus_tag="VFX87_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993767.1"
                     /translation="PYDPRVKSVVEDASYSVAFLADVMARFGLADRWAYVDGVTGICH
                     GLSRERLRDVYREADGLLNVCGATQLKEEHLRCPVRIYIETDPVFEQIRVVEGDRRAI
                     EALEEHTHLFTYGENLGQPDCPIPLEKFAWRPTRPPVIPDLWDATVNPAAERFTTVAT
                     WKNVGKDIRFRGETYYWSKHMNFLRVADLPSRTRQPLELALEVEDAATRDLLARNGWL
                     ITSAFEASRNITAYQRHIERSRGEFTVAKDLVARTRSGWFSDRSVCYLAAGKPVVTQD
                     TGFGKFVPTGVGLLAFETVEEAAAALDEVNHDYARHCRAAREIALEYFAAERVLGRLC
                     READL"
     gene            1133..1762
                     /locus_tag="VFX87_02320"
     CDS             1133..1762
                     /locus_tag="VFX87_02320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4993768.1"
                     /translation="MDAALEKRQVKRAYKLYAPAYDFVFDWIFHPGREAAIRLLAIKA
                     GDNVLEVGIGTGLNLPLYPSNCRLTGIDLSEEMLEKAQDKVVELGLGNVTLKTMDATV
                     MDFGQGEFDSVVATYTISAVPDPVGVLREMKRVVKPGGNIVILNHFRSERPAVGRLED
                     LVAPVCTRLGWKSNLSLEPLLRQVGLVPEITTKVNLFNGWRLIKCVNPR"
     gene            complement(1776..1967)
                     /locus_tag="VFX87_02325"
     CDS             complement(1776..1967)
                     /locus_tag="VFX87_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993769.1"
                     /translation="MSTTGRILAGVVVAVLLLSLAVLELVSERIASSAYRGGVEGGLQ
                     TERIVVLQSGWTEEENPEQ"
     gene            2145..3191
                     /locus_tag="VFX87_02330"
     CDS             2145..3191
                     /locus_tag="VFX87_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993770.1"
                     /translation="MTPTVAGTSAWGAAARLVLAALLALPFFATASTLVLTTSFLVEF
                     IGQGRWHPLSTLTREPVTRALPAAAGTRPVPLDLYARGTLRRPPALVLVHGLSAEGKD
                     DPRLRDAARLLARAGWAVAVPTVEGLTVLRLRPEDAAAVRASVEALHAAGYGRVAVLG
                     ISLGAGPALLAAVDPALAPRLSAVLALGGYASAVELLRYTLTGAYAFGEVAGRAAGVL
                     EPDIAQFARANPDLLDDAGRRLVDNRDPAAVDERIRALPRSTRDLLDALSPGPRIGSL
                     RAPLFLIHGRQDPAVPFTESLRLDAAARAAGRPVRTAIVGAVGHVEADARTGLADLLR
                     LWATFYAFRRAAEG"
     gene            complement(3194..4078)
                     /locus_tag="VFX87_02335"
     CDS             complement(3194..4078)
                     /locus_tag="VFX87_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4993771.1"
                     /translation="MRSLTRAIATLAVVLLGLVADAAPVRAQATPLTLVFTPSRDPTA
                     LKDAGDAFAKTLAEISGVPVRALVASDYAGVIEALRSRRVDLAFVHPVGYVLANREAG
                     CRILVRDVWGGKTAYTARFYVRKDRGIRRVEDLRGKTVAFVDPASSSGYIYPMVLLIK
                     QGLVRERDPKTFFKDALFAGTHEAALQAVLHGRVDAAASFDKAPDLHLKDPALIAQIT
                     FVGETPEIPEAGICVRPGIPDETVARLKRALLSIKAPQHAALLKQIYDIDGFVEADDR
                     DYQPVRDAMQLMNLNPPK"
     gene            4233..4625
                     /locus_tag="VFX87_02340"
     CDS             4233..4625
                     /locus_tag="VFX87_02340"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX4993772.1"
                     /translation="MSRTTQLTLSLVSRPGTLATLARTLADAGVNITALSAAEVTGRG
                     KIRLLVKDPVRAKRALRRAKYRFTEEPAFALRLRNKPGALARVAARLAKERINVRSIY
                     ATTAGRGAAVAVLTVGGNAGKARKVLGR"
     gene            complement(4671..4886)
                     /locus_tag="VFX87_02345"
     CDS             complement(4671..4886)
                     /locus_tag="VFX87_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993773.1"
                     /translation="MADDDLTESIVRQHLRDHPGEAQCAGCLARDLALNPGSTLARIL
                     NDLAERRPPFAPGRCRCGADGLMYVLR"
     gene            complement(4918..6396)
                     /locus_tag="VFX87_02350"
     CDS             complement(4918..6396)
                     /locus_tag="VFX87_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4993774.1"
                     /translation="MGTQECIRKETDGAGDGAAAYRPRPVVLAIDDEPGVRESLQLIL
                     QDEFEVLEAPDGATALAILGARRVDVALLDVRMPGEPGSKVLPRILAIDESIPVILIT
                     ADPHLRMAVDAMKAGAYDYLAKPWDVDEILNLVREAARQRGMERELRYLRAEFDRAHG
                     FDQLVGRHPKMVRLYEMIAQVAQTHATVLVTAESGTGKELVARAIHNQSPRRAQPFVA
                     VNLAAIPDTLLESELFGHEKGAFTGAYSRKPGKFELAHGGTLFLDEIGSLRVDLQAKL
                     LRALQQREIERLGGTRTIQVDVRVIAATNTDLRQAIRARAFREDLYYRLHVVPIGVPP
                     LRERKSDIPDLAAHFVRKYSQEFKKDVRGLSRGARLALDGYDWPGNVRELENIIERSV
                     ALATRPVIGLDDLPLDLAMNEVAPGRGESEVPLTLKAARDRFEQAYVLRALEREDWNQ
                     SRAARGLGVHRNTLIARLAAWGFRRGEPGISVRPVAGGGDPA"
     gene            complement(6497..7975)
                     /locus_tag="VFX87_02355"
     CDS             complement(6497..7975)
                     /locus_tag="VFX87_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX4993775.1"
                     /translation="MTIPCRAVAVPLLLVVLGVLAPPALAQQPRAPQPPRPAAPAPDS
                     QRLLHALEDAFVSVADRATPSVVNVSVKVKREARPESGAAPEMEERFREFFGPELFER
                     FFRRRAPRSEGRAAGSGVLVDPRGYILTNNHVVENAAEIEVRLSDNRKFKATLVGRDG
                     RTDLAVLKIENPVGPLPVADLGDSDRLRVGQWAIAIGNPFGLDRTVTVGIISATGRTH
                     VGVATYEAFIQTDASINPGNSGGPLLNLDGRVVGINTAIVSAGQGIGFAIPINMAREI
                     MTQLINRGRVVRGWLGISIQDLSPELAAGFGVKEDAGVLVADVMKDSPAAAAGFRPGD
                     VIVEFGGSAIKDVPDLQKRVAAIEPGRAAPVKVVREKKTVTLSVKIGEQPTDDAMEAA
                     EDSDEILGLRVEPLTPETAQQNRLAARSGLLVTEVVPGSAGAAAGIRPGDAILEVNRR
                     PVSDAEAFRQAVAGLKPGESVPVYLQRGGGANEYVVLTAPGQ"
BASE COUNT         1195 a         2926 c         2810 g         1201 t
ORIGIN      
        1 tccgtacgac ccgcgcgtca agagtgtggt ggaggacgcg agctacagcg tggccttcct
       61 ggcggacgtg atggcgcgct tcggcctcgc cgaccgctgg gcctacgtgg acggggtgac
      121 cggcatctgc cacgggctct cccgggagcg gctccgcgac gtctaccggg aggcggacgg
      181 cctcctgaac gtctgtggag ccactcagct caaggaagag cacctccgct gcccggttcg
      241 catctacatc gagaccgatc cggtcttcga gcagatccgg gtcgtcgagg gcgatcgccg
      301 ggccatcgag gcgctcgagg agcacacgca cctgttcacc tacggagaga acctgggtca
      361 gcccgactgc cccatcccgc tggagaagtt cgcctggcgc cccacccggc cgcccgtgat
      421 ccccgacctc tgggacgcca ccgtgaaccc tgccgcggag cggttcacga ccgtggccac
      481 ctggaagaac gtgggcaagg acatccggtt ccggggcgag acctactact ggtccaagca
      541 catgaacttc ctccgggtgg ccgacctgcc gagccggacc cgtcagccgc tggagctggc
      601 gctcgaggtg gaggacgcgg cgacacggga cctgctggcc cggaacggct ggctgatcac
      661 gagcgcgttc gaggcctccc gcaacatcac cgcctaccag cgtcacatcg agcgctcccg
      721 aggcgagttc acggtggcca aggacctcgt ggcccgaacg cggagcgggt ggttcagcga
      781 ccgcagcgtc tgctacctgg cagcgggtaa accggtcgtc actcaggaca ccggcttcgg
      841 caagttcgtg cccaccggcg tggggctgct cgccttcgag accgtggagg aggcggcggc
      901 ggcgctggac gaggtcaacc acgactacgc gcggcactgc cgggccgccc gcgagatcgc
      961 cctcgaatac ttcgcggccg agcgggtgct gggccggctc tgccgggagg cggatctgta
     1021 gtattttccc cttgcagatc aataggtcag aaagcacttg gcaatgcgcg tcaccctatg
     1081 ctacaagggc gaaggttggg caatcggcga cgacttctgg aggggtgcca gattggacgc
     1141 agcgctggag aaacggcagg tcaagcgggc ttacaagctg tacgcccccg cgtatgactt
     1201 cgtcttcgac tggatcttcc atccgggtcg ggaagccgcc atccgtctgc tcgccatcaa
     1261 ggccggggac aatgtcctcg aggtcggcat cggcacgggg ctgaatctcc ccctctaccc
     1321 gtccaactgc cggctcaccg gaatcgacct ctccgaggag atgctggaga aggcgcagga
     1381 caaggtggtc gagctggggc tgggcaacgt caccctcaag accatggacg ccaccgtcat
     1441 ggacttcggc cagggcgagt tcgattcggt ggtggccacc tatacgatca gcgcggtgcc
     1501 cgatccggtg ggcgtcctgc gcgagatgaa gcgggtggtc aagccggggg gcaatatcgt
     1561 aatcctgaac cacttccgga gcgagcggcc cgcggtcgga cgcctcgagg atctggttgc
     1621 gccggtctgc acccggctcg gatggaagtc taatctctcg ctcgagccgc tcctgcggca
     1681 ggtgggcctc gtccccgaga tcaccaccaa ggtcaatctc ttcaacggct ggcgcctcat
     1741 caagtgcgtg aatcctaggt agacgtccgc agccgctact gttccgggtt ctcctcctcc
     1801 gtccagccgc tctgtagcac cacgattcgc tcggtctgca gcccgccctc cacgcccccg
     1861 cgatacgccg acgacgcgat gcgctcgctc accagttcca ggacggcgag gctcaggagc
     1921 aagacggcca ccacgacgcc cgccaggatc cggcctgtag tgctcatgtc gcgtcctcct
     1981 cggcgtagca ggagagcatc agcgatgcca gaccataaat actcagaatc gttacgatat
     2041 ccgggcttgc cccggcactc gaaggggcat cctgcctggc cgccggtgga acaatgcccg
     2101 gcgggagagt tgcgctcgat cccgtgcgtt cacatcatgg gcgcgtgacg ccgaccgtgg
     2161 ccggcacgag tgcgtggggg gctgccgcca ggctcgtgct cgcggccctg ctggcgctcc
     2221 ccttcttcgc cacggcgtcc acgctggtcc tgaccacatc cttcctggtc gagttcatcg
     2281 ggcaaggacg ctggcacccg ctctcgacgc tcacccggga gcccgtcacg cgcgcgctcc
     2341 cagccgcggc gggcacgcgc ccggtgcctc tcgatctcta cgcccgcggc accctgcgac
     2401 gcccgcccgc cctcgtcctc gtgcacggtc tctcggccga gggcaaggac gacccgcgcc
     2461 tgcgtgacgc ggcccggctg ctcgcgcggg cgggctgggc ggtggcggtg ccgacggtcg
     2521 aaggcctgac cgtgctgcgg ctccgcccgg aggacgcagc ggccgtgcgc gcgagcgtgg
     2581 aggcgctcca cgccgcgggg tacgggcgcg tggccgtgct cggcatcagc ctgggcgcgg
     2641 ggccggcgct cctggccgcg gtggatccgg cgctggcccc gcgcctttct gccgtcctcg
     2701 cgctcggcgg atacgcttcg gcggtggagc tcctgcgcta cacgctgacc ggggcctacg
     2761 cgttcggcga ggtggcgggc cgggcggccg gggtgctgga gccggacatc gcccagttcg
     2821 cccgcgccaa tcccgacctg ctcgacgacg cggggcgacg cctcgtggac aatcgcgatc
     2881 cggccgcggt ggacgagcgg atccgggcac tgccacggag cacccgggac ctgctggacg
     2941 cgctctctcc tgggcccagg atcggaagcc tccgcgcccc cctcttcctg atccacggcc
     3001 ggcaggatcc ggcggtgccg ttcacggaaa gcctgcggct cgacgcggcc gcgcgcgcgg
     3061 ccgggcgacc cgtgcgcacc gcgatcgtgg gggcggtcgg ccacgtcgag gccgacgcgc
     3121 gcaccggcct ggccgatctg ctgcgactct gggcgacctt ctacgccttc cggcgcgccg
     3181 ccgagggcta ggcttacttc ggggggttca ggttcatgag ctgcatggcg tcgcgcaccg
     3241 gctggtagtc ccggtcgtcc gcctcgacga acccgtcgat gtcgtagatc tgcttgagca
     3301 gcgccgcgtg ctggggcgcc ttgatggaga gaagcgcccg cttgagcctc gccaccgtct
     3361 cgtcggggat cccgggccgc acgcagatac ctgcctccgg gatctcgggc gtctccccca
     3421 cgaaggtgat ctgggcgatc agcgcgggat ccttcaggtg cagatcgggc gccttgtcga
     3481 acgaggccgc cgcgtccacc cgcccgtgga gcaccgcctg gagcgcggcc tcgtgcgtcc
     3541 cggcgaagag cgcgtccttg aagaaggtct tcgggtcgcg ctcgcgcacc agaccctgct
     3601 tgatcagcag caccatcggg tagatgtagc cggaggacga cgcgggatcc acgaaggcca
     3661 ccgtcttgcc gcgcaggtcc tcgacgcgcc ggatgccgcg gtccttccgc acgtagaacc
     3721 gggcggtata ggcggtcttg cctccccaga cgtcgcgcac caggatgcgg cagcccgcct
     3781 cgcgattggc gaggacatat cccaccgggt gcacgaaggc caggtccacg cgccggctgc
     3841 ggagggcctc gatcacgccc gcgtagtccg aggccacgag cgcgcgcacc ggcacgccgg
     3901 agatctcggc cagcgtcttc gcaaaggcat cccccgcgtc cttgagcgcg gtcggatcgc
     3961 gcgacggcgt gaagacgaga gtgagcggcg tggcctgagc cctcaccgga gcagcatcgg
     4021 cgacgaggcc cagcagcacg accgcgagcg tcgcgatggc gcgcgtgaga gagcgcatgc
     4081 gcgcttcaac ctagcacgcg ccgcgagacg ggcgcaaccg acggcgcgtg gcacgcgaac
     4141 tgcccgcccc gaatttttcc cgaagcgggc gctccctggt taaagtgtcc ttactcgatg
     4201 gaacaatgac tcgctcacca ggagggactg cgatgtccag gacgacccaa ctcacgctca
     4261 gcctcgtgtc gcgccccggc acgctcgcca cactggcccg caccctcgcc gacgccggcg
     4321 tcaacatcac ggcgctcagc gccgccgagg tcaccggccg cgggaagatc cggctcctgg
     4381 tcaaggaccc ggtccgggcc aagcgcgcgc tccgcagggc gaagtaccgg ttcaccgagg
     4441 agccggcgtt cgcgttgcgg ctccggaaca agccgggggc gctcgcccgg gtcgcggccc
     4501 ggctcgccaa ggagcggatc aacgtccgat cgatctacgc caccacggcg ggccggggcg
     4561 ccgccgtggc cgtcctgacg gtaggaggca acgcgggcaa ggcacgcaag gtcctgggtc
     4621 gctgagcgcg ccgcctcagc cgccccacga gcccgcgggg gcccggacgc ctagcgcagc
     4681 acgtacatga gcccgtccgc gccgcaccgg caccggccgg gcgcaaacgg gggccgccgc
     4741 tcggccaggt cgttcaggat ccgggcgagc gtgctgccag gattgagggc gagatcccgg
     4801 gccaggcaac cggcacactg tgcctctccg gggtggtctc gcaggtgctg ccgcacgatg
     4861 ctctcggtca ggtcgtcgtc tgccatgtta ggcagcctag cagatgacgc gagctgatca
     4921 cgcgggatcg ccaccccccg ccaccggccg cacgctgatc cccggctcgc cgcggcggaa
     4981 gccccaggcg gccagccggg cgatcagcgt gttgcggtgc acgcccagcc cgcgcgcggc
     5041 gcggctctgg ttccagtcct cgcgctccag cgcccgcagc acgtaggcct gctcgaaccg
     5101 gtcgcgtgcc gccttgaggg tgagcgggac ctcgctctcc ccccggcccg gcgccacctc
     5161 gttcatggcc aggtcgagcg gcaggtcgtc gagcccgatc accgggcggg tggcgagcgc
     5221 cacgctgcgc tcgatgatgt tctcgagctc gcgcacgttg cccggccagt cgtagccgtc
     5281 cagggccagg cgcgcgcccc gcgagagccc gcgcacgtcc ttcttgaact cctgcgagta
     5341 cttccggacg aagtgggcgg ccaggtccgg gatgtcgctc ttgcgctccc gcagcggcgg
     5401 gaccccgatc ggcaccacgt ggagacggta gtagagatcc tcgcggaagg cacgcgcccg
     5461 gatggcctgg cgcaggtcgg tattggtggc cgcgatgaca cgcacgtcca cctggatggt
     5521 gcgggtgccg ccgagccgct cgatctcgcg ctgctggagc gcgcgtagca atttggcctg
     5581 gagatccacc cgcaggctgc cgatctcgtc gaggaagagc gtgcccccat gggccagctc
     5641 gaacttgccg ggcttgcgcg agtacgcacc ggtgaaggcg cccttctcgt ggccgaacag
     5701 ctcggactcg agcaaggtgt cgggaatcgc ggccaggttc acggccacga aaggctgggc
     5761 gcgccgcggg ctctggttgt gaatggcgcg cgccaccagc tccttgccgg tgccgctctc
     5821 cgcggtgacg agcacggtgg cgtgggtctg cgccacctgg gcgatcatct cgtagagccg
     5881 caccatcttc gggtggcgcc ccacgagctg gtcgaagccg tgcgcgcgat cgaactcggc
     5941 ccgcaggtag cgcagctcgc gctccatccc gcgctgccgg gccgcctcgc gcaccagatt
     6001 caggatctcg tccacatccc agggcttggc caggtagtcg tacgcgcccg ccttcatggc
     6061 gtccactgcc atgcgcaggt gcggatcggc ggtgatcagg atgaccggga tggactcgtc
     6121 gatcgccagg atccggggca gcaccttcga cccgggctcg ccgggcatcc gcacgtcgag
     6181 cagcgcgacg tcgacccggc gcgcgccgag gatggcgagg gcggtggccc cgtcgggtgc
     6241 ctccagcacc tcgaactcat cctgcaggat gagctgcagc gactcccgca cgccgggctc
     6301 gtcgtcgatg gcgagcacga cggggcgggg ccgataggcc gccgcgccgt caccggctcc
     6361 gtcggtctct ttccggatac actcctgagt tcccacgcgc gtgtccctcc gtcccggaag
     6421 aaatgaccgg cacgttaggg accgcggcca cgactgtcaa gcggattttc tatccttact
     6481 gcgtgaggaa cggaggtcac tggcccggcg cggtgagcac cacgtactcg ttggcgcccc
     6541 cgccgcgctg gagatacacc ggcaccgact cgccaggctt gagccccgcc accgcctgcc
     6601 ggaacgcctc cgcgtcgctg accggccgcc ggttcacctc gagaatggcg tcacccggcc
     6661 ggatcccggc cgccgcgccc gcgctgcccg gcaccacctc ggtcaccagg agcccgctcc
     6721 gggccgcgag ccggttctgc tgggcggtct cgggcgtgag cggctccacc ctgaggccca
     6781 ggatctcgtc gctgtcctcg gccgcctcca tggcatcgtc ggtcggctgc tcgccgatct
     6841 tgacgctcag ggtaacggtc ttcttctccc gcaccacctt gaccggcgcg gcgcggccgg
     6901 gctcgatcgc cgccacgcgc ttctgcagat cgggcacgtc cttgatcgcc gacccgccga
     6961 actccacgat gacgtcgccg ggccggaatc cggcggcggc ggcggggctg tccttcatca
     7021 cgtcggcaac cagcacgccc gcgtcttcct tcacgccgaa gcccgcggcc agctcggggc
     7081 tcagatcctg gatgctgatg cccagccagc cgcgcaccac cctcccgcgg ttgatgagct
     7141 gggtcatgat ctcgcgcgcc atgttgatcg gaatcgcgaa gccgatgccc tgcccggccg
     7201 agacgatcgc ggtgttgatg ccgaccacgc ggccgtccag gttcaacagc ggcccgcccg
     7261 agttgccggg attgatggac gcgtcggtct ggatgaaggc ctcgtaggtg gccacgccca
     7321 cgtgggtgcg gccggtcgcc gagatgatgc cgacggtcac cgtgcggtcg aggccgaagg
     7381 gattgccgat ggcgatcgcc cactgcccca cccgcaggcg gtccgagtcg cccagatcgg
     7441 ccaccggtag cggaccgacc gggttctcga tcttgagcac cgccagatcc gtacgcccgt
     7501 cgcggcccac gagcgtcgcc ttgaacttgc ggttgtcgga gaggcgcacc tcgatctcgg
     7561 ccgcgttctc caccacgtgg ttgttggtca ggatgtaacc gcgcgggtcc accaggacgc
     7621 ccgagcccgc cgcccggccc tcgctacggg gcgcccgccg gcggaagaag cgctcgaaca
     7681 gctcggggcc gaagaactcg cgaaagcgct cctccatctc gggcgccgcg ccgctctccg
     7741 gccgagcctc gcgcttgacc ttgacgctca cgttcaccac ggagggcgtg gcgcgatccg
     7801 cgacggagac gaacgcgtcc tccagggcgt gcaggagccg ctgcgagtcg ggcgcgggcg
     7861 cggccgggcg cggcggctgg ggcgcgcgcg gctgctgcgc cagggccggc ggcgcgagca
     7921 cgcccaggac cacgaggaga agcgggacgg cgacggcgcg gcacgggatc gtcatgggcg
     7981 ggcctccgcg gaacatctta ccgcccgata cgagggacgg ctactccggg agcaacacca
     8041 gttcgagaac ccgcgtggtg gcggcggtca ccggcgcgat cgagccgcgg cgaatgcccg
     8101 cgacccagat gatctcgccg cccgcctcga cg
//
LOCUS       DASUFA010000063         6870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1268616, whole genome shotgun sequence.
ACCESSION   DASUFA010000063 DASUFA010000000
VERSION     DASUFA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268616"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFX87_02360"
     CDS             complement(<1..993)
                     /locus_tag="VFX87_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4993776.1"
                     /translation="MTTDLAYRTAAELAGLIRARTVSPVEVLQAYLARIDRWDATLHA
                     YVTVCRERALDEARAAEQALARRTPVGPLHGVPFAVKDQFDTAGVRTTSGSRLHADRV
                     PAEDATVVARLRAAGGILLGKLNLTEFALGGTLTYPFGQPRNPWDQTRETGGSSSGSG
                     IAAAAALAAATLGEDTGGSARSPASWCGAVGLRPTWGLVPRSGCVPVAWSMDVPGPIT
                     RSVEDAGLLLSIIAGPDGHDRWAQAAPGDPRADLERGAGGLSIGVVTHLVDSPDTDAS
                     VRTAVREAAETLRRLGARVEDVDLPLLPMAGAVFMALADGEAAAWHLRTLATRGT"
     gene            complement(990..1187)
                     /locus_tag="VFX87_02365"
     CDS             complement(990..1187)
                     /locus_tag="VFX87_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993777.1"
                     /translation="MTELTSDQVRALAAAAGLPLDGPDLAEVTHRLNAFVEVLGGLAA
                     LDLTDAEPAPTLPPDPPRARG"
     gene            1294..1962
                     /locus_tag="VFX87_02370"
     CDS             1294..1962
                     /locus_tag="VFX87_02370"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX4993778.1"
                     /translation="MIQSALLAAYLVFWGAMGLAPLDSQNWVLSSILPLTFVGALVFG
                     RRSLPLSATSYALISTFLTLHTIGAHYTYAQVPLGHWLQGVLGLERNHFDRIVHFAFG
                     LLLTYPLFEAFRRLTGARRPLLFYLAVMTQMGLAGAWEIIESVVAQLTRPDLGAAFLG
                     AQGDPWDAQHDMLAAICGTVVALLVIAVWQRASEGAPGRDPHRAVEPAPGQPVGRSAP
                     EGLA"
     gene            complement(2000..3322)
                     /locus_tag="VFX87_02375"
     CDS             complement(2000..3322)
                     /locus_tag="VFX87_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993779.1"
                     /translation="MTNAVAGLGLAPDAAMVTFPIEMFLPGSDIAPVATRKQEFYDGL
                     TRWRSAFGGDAAGEAPMIRVEATSVEDAIARANDLMLANRWGDGLPLWPPTRTRVDWI
                     LGGAAEPRRRKLGTFPPRGGVTTIESCAIALAMAGGRPEYLPVLVAAVEAFLDPESGS
                     ETLQAASGSAFPVVIVNGPIGAQIRLNAGFGCLGPDPQRPAGASIGRALRLLQQNLGG
                     ALPGIGTMANYGGLRYTNVVFAEDEANLPLGWAPHGTERHGFAPGTSSISLAFANGAT
                     NIRRRGAKKETPEEDALQGMHRMADFMRVPNMAGLAGYEHGTPGILMIPGVVAQTMAG
                     LGWTKASMRAFLWEHSRIPAEELRRAGGPAWIEIDTSKVARESLALEPWPITASPDNF
                     VLVVAGGGHPTNSYWLQGYSPGVVGRPIELPRGFERLLAAAERDLGPR"
     gene            complement(3419..3715)
                     /locus_tag="VFX87_02380"
     CDS             complement(3419..3715)
                     /locus_tag="VFX87_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993780.1"
                     /translation="MMVGLEFYDPSGAIEVKQPHAPRIGALAGKRIGFVSNEQWQAYR
                     MLPLLKQLLEADFPGIEVLPIDAFPQGNAVIGSEETAALVKASGVDAVIIGNAA"
     gene            3886..4368
                     /locus_tag="VFX87_02385"
     CDS             3886..4368
                     /locus_tag="VFX87_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993781.1"
                     /translation="MRVFILTLLAFGLVGPAYAQVHVDIGIHLPRPPRLVVVPEVRSV
                     QYVPTGDANLFFYRGRYWAFANSAWHSSRGYNGPWSVVGRNAVPQSVLLVPVNYYRAR
                     PGNWSQWQRKHPPRWGNEWGHEWAEKRKWHDRGDDHDRGRGRDSDRRADRHDGRGPGR
                     "
     gene            4443..5474
                     /locus_tag="VFX87_02390"
     CDS             4443..5474
                     /locus_tag="VFX87_02390"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4993782.1"
                     /translation="MTITIFGAGAIGGTTGAALAQAGHDVLLVDSNAPHVEAINAQGL
                     TVEREGKATTTRVPAVLARDLGPGLDLVMLAVKSHHTEEALAVLVPKLAAGGTVVSLQ
                     NGLSEEQIARAVGPARTIGCLVNWAADWVGPGRILHGGHGAFVLGELDGRITPRVREL
                     AALLSAVEETPVTDNIWGYKWAKLIYGALLFGTAVVDAHVYEVVERSPGVQQALVGIV
                     GEGLAVADKAGVRVEPFDEFDPAWYRAALAGDTSARARAMTAIAEHYRAHTKTKTGIW
                     RDLAVRKRTTEVDGQLGVLARKGEALGIAMPLTRRLSELIRDLEDGRRQMDWANLDPL
                     VALAGGARA"
     gene            5471..>6870
                     /locus_tag="VFX87_02395"
     CDS             5471..>6870
                     /locus_tag="VFX87_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX4993783.1"
                     /translation="MSRPHWGWQDPLEAALLPGVSADSLMAHARAIATWERESGSPGE
                     ARAFDYIESCLKGYGLEVERREIEAYISLPLESCVLLPDGTVIGALTHSFSTSTGPEG
                     LEAELVDGGDGTVETLARAGARGKLALVDSFATPAKAWAAQEAGTLGQLFANLDHLHN
                     MIVTTIWGTPTPETAGRIPKLPCCSIKRADAESLRARLKAGPLRVRLVARVRTEWMPI
                     PHLTGHLAGAEEAFTLFSGHVDSWHHGAMDNGSANATMLEVARLLAGHRGALRRGLRL
                     AFWSGHSHGRYAGSAWYADHAWRELERNGILHLNVDSTGAKGATDFTVFHATEDAADF
                     AEAVVADVTGQRGRARHFSRAGDQSFWGIGLPSAFMSMSQLPPQDTEFGRTTERLFGT
                     SGFPWWWHTREDTIDKLDGAVLARDTRVFVASALRLCNAPVLPLGPARAARAVADTAA
                     ELHAATAGRMDLSPLV"
BASE COUNT          941 a         2427 c         2522 g          980 t
ORIGIN      
        1 ggtgccgcga gtggccagcg tgcggaggtg ccaggccgcc gcctccccgt cggccagggc
       61 catgaacacc gcgccggcca tcggcagaag cggcaggtcg acgtcctcca cccgcgcgcc
      121 gaggcggcgg agcgtctcgg cggcctcgcg aaccgcggtg cggacggagg cgtcggtgtc
      181 agggctgtcc acgagatggg tgacgacgcc gatcgagagg ccgccggcgc cgcgctcgag
      241 atcggcgcgc gggtcgccgg gcgcggcctg cgcccaccgg tcgtggccgt cggggccggc
      301 gatgatcgag agcagcagcc ccgcgtcctc caccgagcgg gtgatcgggc cgggcacgtc
      361 catggaccac gccaccggca cgcagcccga gcgcgggacg aggccccagg tgggacggag
      421 gccgacggcg ccgcaccacg acgccgggct ccgcgcggag ccgccggtgt cctcgccgag
      481 cgtggccgca gcgagggcgg cggccgcggc gatacccgag cccgaggacg agccgcccgt
      541 ctcgcgcgtc tgatcccacg ggttgcgcgg ctggccgaac gggtaggtga gggtgccgcc
      601 cagcgcgaac tcggtcaggt tcagcttgcc gagcaggatg ccgcccgccg cgcgcagccg
      661 tgccaccacc gtcgcatcct cggcgggcac acggtcagcg tgcagccgcg agcctgacgt
      721 ggtgcgcacg cccgcggtgt cgaactggtc cttcaccgcg aacggtaccc cgtggagggg
      781 gccgaccggc gtccggcggg cgagggcctg ctcggccgcc cgggcctcgt cgagcgcgcg
      841 ctcccggcag accgtgacgt acgcgtgcag cgtggcgtcc cagcggtcga tgcgcgcgag
      901 gtaggcctgc agcacctcca ccggcgagac cgtgcgggcg cggatcaggc cggccagctc
      961 ggccgcggtg cgataggcga ggtccgtggt catccgcggg cacgcggggg atccggaggc
     1021 agggtggggg ccggctcggc gtcggtgagg tcgagtgcgg ccaggccgcc caggacctcc
     1081 acgaacgcgt tcagacgatg cgtgacctcg gccaggtcgg ggccgtcgag cggcaggccg
     1141 gccgcggcgg cgagcgcgcg gacctggtcg gaggtgagct cggtcatgcg aagcgatcct
     1201 accgggcccg cacggccgga gcaatctcca gcttccgctc agcgcccggc acgatcggtt
     1261 gcggggatcc cgctcgcctg gatacaatcg atcatgattc agtcagcgtt gctggccgcg
     1321 tatctcgtgt tctggggcgc catgggtctg gcccccctcg attcccagaa ctgggtgctc
     1381 tcgagcatcc tgcccctgac cttcgtcgga gccctcgtct tcggacgccg gagcctgccg
     1441 ctttcggcga cctcctacgc gctcatttct accttcctca cgctgcacac catcggcgcc
     1501 cactacacgt atgcgcaggt gccgctcggt cactggctcc agggcgtcct cgggctcgag
     1561 cggaaccact tcgaccggat cgtgcacttc gccttcggcc tcctcctcac gtatccgctg
     1621 ttcgaggcct tccggcggct caccggggcc cgccgtcctc ttctcttcta cctggccgtc
     1681 atgactcaga tggggctggc cggggcgtgg gagatcatcg aatcggtggt ggctcagctc
     1741 acgcgacccg atctcggagc ggctttcctc ggggcgcagg gtgacccgtg ggacgcgcag
     1801 cacgacatgc tcgccgcgat ctgcgggacg gtggtcgcgc tgctggtcat cgcggtctgg
     1861 cagcgagcga gcgaaggggc gccggggcgc gaccctcatc gtgccgtcga gccggcgccg
     1921 ggccagccgg tggggcgatc ggcgccggag gggctggcct aggccccgcg acgcgggtcg
     1981 caccgatccc ccagcgtccc tagcgaggtc ccaggtcgcg ctcggcggcg gcgagcaggc
     2041 gctcgaaccc ccgcggcagc tcgatcgggc ggccgacgac gccagggctg tagccctgca
     2101 gccagtagct gttggtcggg tggccgcctc cggccacgac cagcacgaag ttgtcggggc
     2161 tggcggtgat cggccatggc tccagggcga gactctcgcg ggccaccttg ctcgtgtcga
     2221 tctcgatcca cgccggacct cccgcgcgac ggagctcctc ggccggaatc cgtgaatgct
     2281 cccagaggaa cgcgcgcatc gaggccttcg tccagccgag gccggccatg gtctgggcca
     2341 cgacgcccgg gatcatcagg atgcccgggg tgccgtgctc gtagccggcg agcccggcca
     2401 tgttcggcac gcgcatgaag tcggccatgc ggtgcatgcc ctggagcgcg tcctcctccg
     2461 gcgtctcctt cttggcgccg cggcgccgga tgttcgtggc cccgttggcg aaggcgagcg
     2521 agatcgagct ggtgcccggc gcgaagccgt gccgctcggt tccgtgcggc gcccagccca
     2581 ggggcaggtt cgcctcgtcc tcggcgaaga ccacgttcgt gtagcgcagg ccgccgtagt
     2641 tggccatggt gccgatgccg ggcagggcgc cgcccaggtt ctgctggagc aggcgcagcg
     2701 cgcggccgat gctggccccc gccggtcgtt gcggatccgg gcccaggcag ccgaagcccg
     2761 cgttcaggcg gatctgggcg ccgatcggcc cgttgacgat caccaccgga aaggcgctgc
     2821 ccgatgcggc ctggagcgtc tcgctccccg actcgggatc gaggaaggcc tccaccgcgg
     2881 ccacgagcac gggcaggtac tccggccggc cgcccgccat cgccagcgcg atggcacacg
     2941 actcgatggt ggtcacgccg ccgcgcgggg gaaaggtgcc gagcttgcgc cggcgcggtt
     3001 ccgcggcgcc accgaggatc cagtccacgc gcgttcgcgt cgggggccag agcggcaggc
     3061 catcgcccca gcggttggcg agcatcagat cgttggcccg cgcgatggca tcctcgacgc
     3121 tcgtcgcctc cacgcggatc atgggcgcct cgcccgcggc gtcgccgccg aaggccgagc
     3181 gccaccgggt cagcccgtcg tagaactcct gcttacgcgt ggccaccggg gcgatgtcgc
     3241 tgcccggcag gaacatctcg atggggaagg tgaccatggc cgcgtccggc gccagtccga
     3301 ggccggcaac cgcattcgtc atgaccccaa cgaaatcgcc gcgggtcagc gtgacggttg
     3361 gaatcccctg tgtttcgagc ctggcggccg cccggccgca cgcggtcgcg caggtccctc
     3421 aggcggcgtt gccgatgatg acggcgtcaa ccccgctcgc cttgacgagc gccgccgtct
     3481 cctccgagcc gatcaccgcg ttgccctggg ggaaggcatc gatcggcagg acctcgatgc
     3541 cggggaagtc cgcctcgagc agctgcttga gcagcggcag catgcggtag gcctgccact
     3601 gctcgttgct gacgaagccg atccgcttgc ccgcgagggc gccgatgcgg ggggcgtggg
     3661 gctgcttcac ctcgatggcg ccggacgggt cgtagaactc gaggccgacc atcacgaagc
     3721 cctcctggaa acgtcgtagc cgcgcgccga gtatagccca aggggcaggg gggcacggct
     3781 gcctattgga cgttcgccgg tagaccaagg tccaatacgg atgccgatgg gccgtggcta
     3841 gagttgtaag gagatatcgg acagcttgcc attggaggct catacatgcg tgtattcatc
     3901 cttactttgc tcgcgttcgg cctcgtcggg ccggcgtatg ctcaggtgca tgtcgacatc
     3961 ggcatccatc tgccgcggcc gcctcggctc gtcgtcgtcc ccgaagtccg gtcggtccag
     4021 tacgtaccga ccggtgacgc gaacctcttc ttctatcgcg gccggtattg ggccttcgcc
     4081 aacagcgcct ggcactcgag tcgcggatac aacggcccct ggtccgtcgt cggccgaaac
     4141 gccgtgccac aatccgtgct gctcgtgccc gtgaactact accgcgcgcg ccccggaaac
     4201 tggagccaat ggcagcggaa gcaccctccg cgctggggca acgagtgggg ccatgagtgg
     4261 gcggaaaagc gtaagtggca cgatcggggt gatgatcatg accggggccg cggccgggac
     4321 agcgatcggc gtgcggaccg gcacgatggc cgggggcccg ggcggtaagt agcaccttca
     4381 aggtcgggct gaccctccgc gcttgaccca cgtctctcgc gtgtgttagg tccgagggca
     4441 ccatgaccat caccatcttc ggtgcgggcg cgatcggagg gaccaccggg gccgccctgg
     4501 cccaggcggg gcacgacgtt ctgctcgtgg acagcaacgc gccgcacgtc gaggccatca
     4561 acgcccaggg cctcaccgtg gagcgcgagg gcaaggccac caccacgcgg gtgccggcgg
     4621 tgctggcccg ggacctcggc cccggcctgg acctcgtcat gctggcggtg aagagccacc
     4681 acaccgagga ggcgctcgcc gtccttgtcc ccaagctggc ggcgggcggc accgtggtgt
     4741 cgctgcagaa cggtctcagc gaggagcaga tcgcccgcgc ggtggggccg gcgcgcacca
     4801 tcggttgcct ggtgaactgg gccgcggact gggtgggccc gggccgcatc ctccacggcg
     4861 gccacggggc ctttgtgctg ggcgagctcg acggccgcat caccccgcgg gtgcgagagc
     4921 tggccgcact gctgagcgcg gtggaggaga ccccggtcac ggacaatatc tggggctaca
     4981 agtgggccaa gctgatctac ggcgcactcc tcttcggcac cgcggtggtg gacgcccacg
     5041 tgtacgaggt cgtggagcgg agccccggcg tgcagcaggc gctggtgggc atcgtgggcg
     5101 aggggctggc ggtggccgac aaggccgggg tgcgcgtcga gcccttcgac gagttcgatc
     5161 ccgcatggta tcgcgccgcg ctggccggtg acacgagcgc gcgcgcccgc gcgatgaccg
     5221 ccatcgccga gcactatcgc gctcacacca agaccaagac cggcatctgg cgtgacctcg
     5281 cggtccgcaa gcgcacgacc gaggtggacg gccagctcgg cgtgctggcg cggaagggcg
     5341 aggccctcgg tatcgccatg ccgctgaccc gccggctcag cgagctgatc cgcgatctcg
     5401 aggacggccg gcgccagatg gactgggcca acctcgatcc gctcgtcgcc ctggcgggcg
     5461 gggcgcgcgc gtgagccgtc cgcactgggg ctggcaggat ccactggagg ccgcgctcct
     5521 ccccggggtg tcggccgatt cgctgatggc ccacgcccgc gccatcgcca cctgggagcg
     5581 cgagtcgggc agcccgggcg aggcgcgcgc cttcgactac atcgagagct gcctcaaggg
     5641 ctatggcctg gaggtggagc ggcgcgagat cgaagcgtac atctcgctac cgctcgagtc
     5701 ctgcgtgctc ctgcccgacg gtaccgtgat cggggcgctc acgcactcct tctccacgtc
     5761 caccgggccg gagggcctcg aggccgagct ggtggacggc ggcgacggca ccgtggagac
     5821 gctggcgcgc gccggcgcgc ggggcaagct ggccctggtg gacagcttcg ccaccccggc
     5881 gaaggcgtgg gccgcgcagg aggcaggcac gctgggccag ctcttcgcca atctcgacca
     5941 cctgcacaac atgatcgtca ccacgatctg gggcacgccc accccggaga ccgcggggcg
     6001 catcccgaag ctgccgtgct gctcgatcaa gagggcggac gcggagagcc tgcgcgcccg
     6061 gctcaaggcg ggcccgctcc gcgtgcggct ggtcgcccgc gtgcgcaccg agtggatgcc
     6121 cattccgcat ctcaccgggc atctggccgg agcggaggag gcgttcacgc tcttctccgg
     6181 ccacgtggac tcgtggcatc acggcgccat ggacaacggc agcgccaatg ccaccatgct
     6241 cgaggtcgcg cggcttctgg ccgggcatcg gggcgcgctg cgccgggggc tgcgcctggc
     6301 cttctggtcc ggccactcgc acgggcgcta cgcgggctcg gcgtggtacg cggaccacgc
     6361 gtggcgcgag ctcgagcgca acgggatcct gcacctcaac gtggattcga cgggcgccaa
     6421 gggcgcgacc gacttcaccg tgttccacgc caccgaggac gcggccgact tcgcggaggc
     6481 ggtcgtggcg gacgtgaccg gccagcgagg ccgggcgcgg cacttctcgc gcgcggggga
     6541 ccagtccttc tgggggatcg ggctacccag cgccttcatg tccatgtcgc agctcccgcc
     6601 gcaggacacc gagttcggcc ggaccaccga gcggctcttc gggacctccg ggttcccgtg
     6661 gtggtggcac acgcgcgagg acacgatcga caagctcgac ggcgccgtgc tggcccgcga
     6721 cacgagggtc ttcgtggcct cggccctgcg gctctgcaat gcgccggtct tgccgctggg
     6781 gccggcccgc gccgcgcgcg cggtggcgga taccgcggct gagctgcatg ccgcgacagc
     6841 cgggcgcatg gatctctcgc cgctggtgga
//
LOCUS       DASUFA010000064         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_816181, whole genome shotgun sequence.
ACCESSION   DASUFA010000064 DASUFA010000000
VERSION     DASUFA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816181"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFX87_02400"
     CDS             <1..562
                     /locus_tag="VFX87_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4993784.1"
                     /translation="ARLMKILDAGAYGVICPMINTAQEADAMVRACKYPPRGFRSWGP
                     VRASLYAGSDYGDHANVETIVMPMIETAEALRNLDEILSVDGVDAIYVGPSDLSLAMG
                     LKPRLDQTDPPVVDAQKKIAAACKRHNVVAGIHNATATYALKMIAEGYQFVTLASDSR
                     FMAAKAAEEVGIVRKTGTKAGKLPAY"
     gene            562..1185
                     /locus_tag="VFX87_02405"
     CDS             562..1185
                     /locus_tag="VFX87_02405"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX4993785.1"
                     /translation="MPARLDGGLLDRARANLSSFERQALPDDGRRRAAVAQVLLPDGG
                     GRPCFLITRRAATLRKHTGQWALPGGRLDAGETAAQAALRELQEEVGLTLDESTVLGV
                     LDDYGTRSGFIITPVVVWAEPDHELVPNPAEVAKIFHVPLDDLEGPDVPRLINIPESD
                     RPVIQLPLLGTLIHAPTAAVLYQMREVVVHGRSTRVNHFEQPVWAWR"
     gene            1214..2002
                     /locus_tag="VFX87_02410"
     CDS             1214..2002
                     /locus_tag="VFX87_02410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4993786.1"
                     /translation="MGVLDGQVAIMTGGGTGIGRAAALMLAAEGAQVVVAGRRKAPIE
                     AVAGEIAKAGGRAVARVCDVAKPAEARALAEWTVAQYGRVDVLVNNAGHSSKRRNVRW
                     VEQDDWDAVVAVNLTGVYALTQAVLPSMIERGGGTIITVSSLAALKPGLIGGAPYGAA
                     KAGVRNLMGHVHTVLREKGIRATTIMPAEVDTPILDNRPLPPDAKARATMMQAEDVAQ
                     AIMLCVTLPARTVIEEMVMSPTILRDQSADLKTARDLGATPGAD"
     gene            complement(2052..2534)
                     /locus_tag="VFX87_02415"
     CDS             complement(2052..2534)
                     /locus_tag="VFX87_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX4993787.1"
                     /translation="MGYVERHLLPGERVLYKTRLHWGVFIKPALVVLVGVALMVLLRQ
                     VPDPRWLWMVGAAVALIGLVWGLVHYVEVMTSEFAVTTSRLIFKVGLISRYTTELLLA
                     KVESIGVQQGLVGRMLSYGDLTVTGTGGAREVFRRVRDPIGFRNHVQQASLGAGPPGA
                     "
     gene            complement(2549..>2819)
                     /locus_tag="VFX87_02420"
     CDS             complement(2549..>2819)
                     /locus_tag="VFX87_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4993788.1"
                     /translation="ACTVMNNSRIGIGAVVLDGAVVEEGAQVGAGALVPPGKVVPAGW
                     LVMGVPAKPVRKMSQEELDDILRNATDYVELWHRDYRDLYGRRPG"
BASE COUNT          440 a         1022 c         1012 g          345 t
ORIGIN      
        1 ggcacggctc atgaagatcc tcgacgcggg cgcctacggc gtcatctgcc cgatgatcaa
       61 caccgcgcag gaggcggacg cgatggtgcg cgcgtgcaag taccccccgc gcggcttccg
      121 gagctggggc ccggtgcgtg cctcgctcta cgccgggtcc gattacggtg accacgccaa
      181 cgtcgagacc atcgtcatgc cgatgatcga gaccgcggag gcgctccgaa acctcgacga
      241 gatcctgagc gtggacggcg tggacgccat ctacgtgggc ccctccgacc tgagcctggc
      301 catggggctc aagccccggc tcgaccagac cgatccgccg gtggtggacg cccagaagaa
      361 gatcgcggcg gcgtgcaagc ggcacaacgt cgtcgcgggg atccacaacg ccaccgcgac
      421 ctacgcgctc aagatgatcg cggagggcta ccagttcgtc accctggcca gcgacagccg
      481 cttcatggcc gccaaggccg ccgaggaagt gggcatcgtg cggaagaccg gcaccaaggc
      541 cgggaagctg ccggcctact agtgccggcg cggctggacg gcgggctgct cgaccgggcg
      601 cgcgccaatc tgtcgtcgtt cgagcggcag gccctgcccg acgacgggcg gcggcgggcg
      661 gcggtggccc aggtgctgct gcccgacggg ggcggccgcc cgtgctttct catcacgcgg
      721 cgggccgcca ccctgcgcaa gcacacgggc cagtgggcgc tgccgggcgg ccgcctcgac
      781 gcgggcgaga ccgcggcgca ggccgcgctg cgcgagctgc aggaggaggt gggcctgacg
      841 ctcgacgaga gcaccgtgct cggcgtgctg gacgactacg gcacccgatc gggcttcatc
      901 atcaccccgg tggtcgtgtg ggccgagccg gaccacgagc tcgtgccgaa cccggcggag
      961 gtcgccaaga tcttccacgt gccgctcgac gatctggagg ggccggacgt gccgcgcctc
     1021 atcaacatcc cggagagcga ccggccggtg atccagctcc ccctgctcgg caccttgatc
     1081 cacgcgccga ccgcggccgt gctctaccag atgcgcgagg tggtcgtcca cggacggtcg
     1141 acgcgcgtga accatttcga acagcccgtc tgggcctggc gctgagccgg acccgcgggc
     1201 cggaggacaa ccgatgggag tgctcgacgg acaggtcgcg atcatgacgg gaggcggtac
     1261 cggcatcggc cgcgcggcgg cgctgatgct ggccgccgag ggcgcacagg tggtggtggc
     1321 ggggcggcgc aaggcgccga tcgaggcggt ggccggcgag atcgccaagg cgggcgggcg
     1381 cgcggtggcg cgggtctgcg acgtggcgaa gccagcggag gcccgcgcgc tcgccgagtg
     1441 gacggtggcc cagtacggcc gggtggacgt gctcgtcaac aacgcgggcc actccagcaa
     1501 gcgccgcaac gtgcgctggg tcgagcagga cgactgggac gcggtggtcg cggtgaacct
     1561 gaccggggtc tacgccctca cccaggcggt gctacccagc atgatcgagc ggggcggcgg
     1621 caccatcatc acggtgagct cgctggccgc gctgaagccc gggctgatcg gcggggcgcc
     1681 ctacggcgcg gccaaggccg gcgtccggaa cctgatgggc cacgtccaca cggtgctgcg
     1741 ggagaagggc atccgcgcca ccaccatcat gcccgccgag gtggacaccc cgatcctcga
     1801 caaccgcccg ctgccgcccg acgccaaggc gcgcgccacg atgatgcagg ccgaggacgt
     1861 ggcccaggcg atcatgctct gcgtgacgct gcccgcgcgc accgtgatcg aggagatggt
     1921 gatgagcccg acgatcctgc gtgaccagag cgccgatctc aagaccgcgc gcgacctcgg
     1981 ggccacgccg ggggcagact agccgcgccc ccgcgcggcc ctcggctcgc cttctcggcg
     2041 aaaggggcgg ctcaggcgcc gggcgggccc gcccccagcg acgcctgctg cacgtgattg
     2101 cggaagccga tcgggtcccg cacgcgccgg aagacctcgc gggcgccccc ggtgccggtg
     2161 acggtgagat cgccgtagct gagcatgcgg ccgaccagcc cctgctgcac gccgatcgac
     2221 tccaccttgg cgaggagcag ctcggtggtg tagcgcgaga tcagccccac cttgaaaatg
     2281 agccgcgagg tggtgaccgc gaactcggag gtcatgacct cgacgtagtg gacgaggccc
     2341 cagacgagcc cgatcagcgc gaccgccgcg ccgaccatcc agagccaccg cgggtccggc
     2401 acctggcgga gcagcaccat gagggccacc ccgaccagca caaccagggc cggcttgatg
     2461 aacacccccc agtgcagccg cgtcttgtac agcacgcgct cccccggcag gaggtgccgc
     2521 tccacgtacc ccacgccgtg ggcctacctc accccgggcg acggccgtag aggtcgcggt
     2581 agtcgcggtg ccagagctcg acgtagtccg tcgcgttgcg gaggatgtcg tccagctcct
     2641 cctggctcat cttgcgcacc ggcttggccg gcactcccat gacgagccag cccgccggca
     2701 ccaccttgcc cggcggcacc agcgcgcccg cgcccacctg cgcgccctcc tccaccaccg
     2761 ccccgtccag caccaccgcg ccgatgccga tgcgcgagtt gttcatcacg gtgcaggcg
//
LOCUS       DASUFA010000065         6736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_931045, whole genome shotgun sequence.
ACCESSION   DASUFA010000065 DASUFA010000000
VERSION     DASUFA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931045"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..77)
                     /locus_tag="VFX87_02425"
     tRNA            complement(2..77)
                     /locus_tag="VFX87_02425"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Phe,seq:gaa)
     gene            complement(180..515)
                     /locus_tag="VFX87_02430"
     CDS             complement(180..515)
                     /locus_tag="VFX87_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993789.1"
                     /translation="MDEAQRRAKLQELYDLAQGSEEFDGGITWEPETEALVVGNWAFF
                     AIDEIGDLALSFHLDSHPVAVAKLTRFLVQHEVPFVLHEAFTVDDDDRIVFESDTGAE
                     FDERGKPQA"
     gene            complement(515..1852)
                     /locus_tag="VFX87_02435"
     CDS             complement(515..1852)
                     /locus_tag="VFX87_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4993790.1"
                     /translation="MTSASSKVVETYRAMHPKSAALYERARAIIPGGVTHDGRHMKPF
                     PVYVERALGSHKWDVDGHDYVDYWMGHGALFLGHCHPKVVKAIQEQAARGTHFGASHE
                     LEVRWAELITKLIPSAEMVRFAMSGTEATHLALRIARAYTGRNKVVKFQGHFHGWHDG
                     VVAAVNPPYDVPMSAGVPTSVLDQLLVCPPNDIKSVDTMLERGDVAAVILEPAGGQSG
                     TTPTIPGYLQELRSLTTRHKVVLIFDEVITGFRYAPGGAQAYFGVTPDMTTLAKIVAG
                     GLPGAAVVGKKELLSMMAHRGEPVFDRSQRVAQNGTFNSNPVCAAAAIATLELVGDGS
                     LHARANKAGDELRSGLSDAMKRAGVPGTCFGEASIFHVSFEGKPGLAGFDRPRRGDLY
                     HLLRCALLNNGVDCASHHGWISAVHTDADIERTIAAHEKAFREMAADGVFKGM"
     gene            complement(1849..2739)
                     /locus_tag="VFX87_02440"
     CDS             complement(1849..2739)
                     /locus_tag="VFX87_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HEX4993791.1"
                     /translation="MIDQLMALDPGGRGIAAFFVRGGASAAARALRRSRRVLLTTGFM
                     VGPGLPETDGPPGAACLGRALRALGAEVTYVTDAVALPPLQASLKVLGEPAAVLTFHV
                     PHAASGGPEPGAAREAARRLLAEHRPTHLVAIERPGRARDGEYRSARGQSLTEWNGPL
                     DELFLAAPARTVTIGIGDGGNEIGMGTVHARVARAGAVFRAIASVVAVRHLVVAGVSN
                     WGAYGVVAELARLAGRPLLHTAEEEEAMVRACVEAGAVDGLTRRAEPTVDGLPLPAHI
                     GMLELLRTMESPRGTGGPRK"
     gene            complement(2740..3267)
                     /locus_tag="VFX87_02445"
     CDS             complement(2740..3267)
                     /locus_tag="VFX87_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993792.1"
                     /translation="MATGIARAFLAGLAIAALLVGPAGARAEEWGGIEPGLTTSEQVR
                     ARYGAPSKETRAKVEGHDTIQWVFEDTRAPGGIQRLTVDYGLLTPQGYKPTVVRAFRL
                     EPKPKVFAKNTVAQAWGPPDAITSQNEQETFFYKSGLVVIFTKDGTDTVLMTFTPPQP
                     DRPTPAGPTPAAPKR"
     gene            complement(3267..4862)
                     /gene="ggt"
                     /locus_tag="VFX87_02450"
     CDS             complement(3267..4862)
                     /gene="ggt"
                     /locus_tag="VFX87_02450"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4993793.1"
                     /translation="MPQIGYAHRQAPMGRRGMVAACHPLASLAGVDVLKSGGNVVDAA
                     VATNAVLAVTQPNFCGVGGDIFCLYYEAATKRVHFLSGAGRSGARATLEELGRRGLTA
                     VPGVGPGSVSVPGVTRGWRMLLDRFGTRPLASLLEPAIHYAGEGFPLSDVVAQAIREK
                     APAADDPEWHRVFVPGGKFPQTGDCFRQPDLARTLTELGGEPELFYKGRVARSIAARM
                     AREGFLTAEDLAAHEGEWGEPISSTYRGYTVYETPPPTQGLAALLTLNLLEGVDLTRY
                     EIHSPEHLHLLIEMTKLAYADRNRWIADPAHARVPVEALLSKGYAEKRRRAFDPGRAQ
                     DHRWGAIDGDTTGFVVADGRGNIMSVIQSLYKAFGSVVVAPDTGVVLQNRGAYFNTDP
                     THPNCFAPGKRPFHTLIACVVTKGDDPVLGYSNMGGDGQAMFHAQSLTNALDYGMEIQ
                     EAIERPRFVAGPIDPGDVADQVRIEGRVPPATREALGRMGHNVTPVSDFFMRMGHAHG
                     ITLASGTLRGGADPRGDGAAIGF"
     gene            complement(4864..5883)
                     /locus_tag="VFX87_02455"
     CDS             complement(4864..5883)
                     /locus_tag="VFX87_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HEX4993794.1"
                     /translation="MPPLSVGKLPPQLLQRLLSAQASDDPRVVVGPQVGEDAAVLDIG
                     ERYLVATTDPITFATDELGWYALHVNANDLAVRGARPLWFLATVLLPEGRATELGVER
                     LFTELREACAELGVALVGGHTEVTTGLPRPIVSGCMLGEVEKDRLVTTGGARPGDVLL
                     LTKGVPLEGAAIIARERAAEAERRGVSADVVKRARDFLRNPGISVVPEARAACAAARV
                     HAMHDPTEGGVATACWELAQAADVGLRVDRERIPVLHEGRVLCEAFGLDPLGTIASGS
                     LLLAVDPADAAGVAAACRGAGVDCAPIGRVTEASQGVSLATGGHARPMPTFPQDQITR
                     IFSEG"
     gene            complement(6127..>6736)
                     /locus_tag="VFX87_02460"
     CDS             complement(6127..>6736)
                     /locus_tag="VFX87_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX4993795.1"
                     /translation="LAGMTMVTGNLMAIPQQNIKRLLAYSGVAHIGYMLIGLAAVSAN
                     GVAMMLFYLVAYLFGNMGAFLVVEAVSQAEGSEGITAYRGLAQRSPILALCMLLFLLS
                     LGGIPFVAGFWAKLYVFWAAAEQGLYWLVLVGAVLTVVALFYYLLIAKSMYIDAPERR
                     DPIPVAPLLSFCIFLCVLGVVIMGLYPKPLVVAALRAAGPLF"
BASE COUNT          999 a         2351 c         2445 g          941 t
ORIGIN      
        1 atggtgggct gggacgggat tgaaccgccg acaccaggat tttcagtcct gtgctctacc
       61 aactgagcta ccagcccacc acgagttttc gaggatagca gaacggggtg gaagtgtcta
      121 cggagactgc ctaaaacggc ccctcaccct gccctctccc cggtgggaga gggggtgagt
      181 caggcctggg gcttgccgcg ctcgtcgaac tcggcgccgg tgtcggactc gaagacgatg
      241 cggtcgtcgt cgtccaccgt gaaggcctcg tgcagcacga agggcacctc gtgctggacc
      301 aggaagcgcg tcagcttggc caccgccacc gggtgcgagt cgaggtgaaa cgagagcgcc
      361 aggtcgccga tctcgtcgat ggcgaagaag gcccagttgc cgaccacgag cgcttcggtc
      421 tcgggctccc aggtgatgcc gccgtcgaac tcctcggagc cctgggccag gtcgtagagc
      481 tcctggagct tggcgcggcg ctgggcctcg tccactacat gcccttgaag acgccgtcgg
      541 cggccatctc gcggaaggcc ttctcgtggg ccgcgatggt gcgctcgatg tcggcgtcgg
      601 tgtgcacggc ggagatccag ccgtggtggc tggcgcagtc cacgccgttg ttgagcaggg
      661 cgcagcgcag aaggtggtag agatcgccgc ggcgtgggcg gtcgaagccg gcgaggccgg
      721 gcttgccctc gaacgagacg tggaagatgg acgcctcgcc gaagcaggtg ccggggacgc
      781 cggcacgctt catcgcgtcc gacaggcccg agcgcagctc gtcgcccgcc ttgttcgcgc
      841 gcgcgtgcag cgagccgtcg ccgaccagct cgagcgtggc gatggcggcg gcggcgcaca
      901 ccgggttgga gttgaacgtg ccgttctggg ccacccgctg gctccggtcg aagaccggct
      961 cgccgcggtg ggccatcatc gagagcagct ccttcttgcc gacgacggcg gcgccgggca
     1021 ggccgcccgc cacgatcttg gccagcgtgg tcatgtcggg ggtcacgccg aagtaggcct
     1081 gggcgccgcc gggcgcgtag cggaacccgg tgatcacctc gtcgaagatc agcaccacct
     1141 tgtgccgcgt ggtgagactg cgcagctcct ggagataccc cgggatcgtg ggcgtggtgc
     1201 cggactgccc acccgccggc tccaggatca ccgccgcgac gtccccgcgc tcgagcatcg
     1261 tgtccaccga cttgatgtcg ttgggcgggc acacgagcag ctgatcgagg acggaggtcg
     1321 ggacgccggc cgacatcggc acgtcgtacg gcgggttcac cgcggcgacg actccgtcgt
     1381 gccagccgtg gaagtggccc tggaacttca cgaccttgtt gcgcccggtg taggcgcgcg
     1441 cgatgcgcag ggccagatgc gtggcctcgg tgcccgacat ggcgaagcgc accatctcgg
     1501 ccgaggggat cagcttggtg atcagctcgg cccaacgcac ctcgagctcg tggctggcgc
     1561 caaagtgcgt gcctctcgcg gcctgctcct ggatggcctt caccaccttc gggtggcagt
     1621 ggcccaggaa gagcgcgccg tgacccatcc agtagtccac gtagtcgtga ccgtccacgt
     1681 cccatttgtg cgagccgagg gcgcgctcca cgtagaccgg gaacggcttc atgtgccgcc
     1741 cgtcgtgcgt gacgccgccg gggatgatgg cccgtgcgcg ctcgtagagc gcggccgact
     1801 tgggatgcat ggcgcgatag gtctcgacca ccttggacga cgcactggtc atttcctcgg
     1861 gcctccggta ccgcggggtg attccatcgt gcgcaacagc tcaagcatgc cgatatgggc
     1921 cggcaaaggc aagccgtcca cggtgggctc ggcccgccgc gtgaggccgt ccaccgcgcc
     1981 cgcctccacg caggcgcgca ccatcgcctc ttcttcctcg gcggtgtgca gcagcgggcg
     2041 gccggccagc cgcgccagct cggcgacgac cccgtaggcg ccccagttgg atacgcccgc
     2101 gaccacgagg tgccgcactg ccaccaccga ggcgatggca cggaagacgg ctccggcgcg
     2161 cgccacgcgg gcgtgcaccg tgcccatgcc gatctcgttg ccgccgtcgc caatgccgat
     2221 cgtgacggtc cgcgccggcg cggccaggaa cagctcgtcg agcgggccgt tccattcagt
     2281 gagcgactgg ccgcgcgcgc tccgatactc gccgtcgcgc gcgcgccccg gccgctcgat
     2341 ggcgacgagg tgcgtgggcc ggtgctcggc caggagccga cgcgcggcct cgcgcgcggc
     2401 gccgggctcg ggcccgccac tcgcggcatg cggcacgtgg aaggtcagca cggccgcggg
     2461 ctcgcccagg accttcagcg aggcctgcag cggcggcagc gccaccgcgt cggtgacgta
     2521 ggtaacctcc gcccccagcg cgcggagcgc gcggccgagg caggcggcgc cgggcggccc
     2581 gtcggtctcc ggcaggccgg ggcccaccat gaagcccgtg gtcaggagca cgcggcggct
     2641 ccggcgcagg gcgcgggccg ccgcgctggc gccgccgcgc acgaagaacg cggcgatgcc
     2701 ccggccgccg gggtccaggg ccatgagctg atcgatcatc tagcgcttgg gcgcggcggg
     2761 ggtcgggccg gccggggtcg gccggtcggg ctgaggcggc gtgaaggtca tcagcaccgt
     2821 gtcggtcccg tccttggtga agatgaccac gaggccggac ttgtagaaga aggtctcctg
     2881 ctcgttctgg ctggtgatgg cgtcgggcgg gccccaggcc tgcgcgacgg tgttcttggc
     2941 gaagaccttg ggcttgggct cgagccggaa cgcgcgcacc accgtcggct tgtagccctg
     3001 cggcgtgagc aggccgtagt ccacggtcag gcgctggatg ccgcccggcg cgcgcgtgtc
     3061 ctcgaagacc cactggatcg tgtcgtgacc ctccaccttc gcgcgcgtct ccttgctcgg
     3121 cgcgccgtag cgtgcgcgga cctgctcgct cgtggtgagg ccgggctcga tgccacccca
     3181 ttcctcggcg cgggcgccgg ccggccccac gagcagcgcg gcaatcgcga gcccggccag
     3241 gaacgcgcgc gcgatcccgg tggccatcaa aagccgatcg cggcgccgtc gccgcgggga
     3301 tccgcgccgc cgcgcagcgt gccgctggcg agcgtgatgc cgtgcgcgtg gcccatgcgc
     3361 atgaagaagt cggagacggg cgtgacgttg tggcccatgc ggccgagcgc ctcgcgggtg
     3421 gccgggggca cccggccctc gatccgcacc tgatccgcga cgtcgcccgg atcgatgggc
     3481 ccggccacga agcgcggccg ctcgatcgct tcctggatct ccatgccgta gtcgagcgcg
     3541 ttggtgagcg actgggcgtg gaacatcgcc tgcccatcgc cgcccatgtt cgagtagccg
     3601 agcaccgggt cgtccccctt cgtgaccacg caggcgatca gggtgtggaa cgggcgcttg
     3661 cccggggcga agcagttggg gtgcgtggga tcggtattga aataggcgcc gcggttctgc
     3721 agcaccacgc cggtgtcggg cgcgaccacg accgagccga acgctttgta gaggctctgg
     3781 atgacgctca tgatgttgcc gcgcccgtcg gccaccacga agccggtggt gtcgccgtcg
     3841 atcgcgcccc accgatggtc ctgcgcgcgg cccgggtcga aggcccgccg ccgcttctcc
     3901 gcgtaaccct tgctcagcag ggcctccacc ggcacgcgcg cgtgcgccgg atcggcgatc
     3961 cagcggttgc ggtcggcgta ggccagcttg gtcatctcga tcagcaggtg gagatgctcg
     4021 ggcgagtgga tctcgtaccg cgtgagatcg acgccctcga gcaggttcag cgtcagcagc
     4081 gcggccaggc cctgcgtggg cggcggcgtc tcgtacaccg tgtagccgcg gtaggtggac
     4141 gagatcggct cgccccactc gccctcgtgc gcggccaggt cctccgcggt gaggaagccc
     4201 tcgcgcgcca tgcgggccgc gatcgagcgc gccacgcgtc ccttgtagaa gagctcgggc
     4261 tcgccgccca gctcggtgag cgtgcgcgcg agatcgggct ggcggaagca atcgccggtc
     4321 tgcgggaact tgccgccggg cacgaagacg cggtgccatt ccggatcgtc ggcggcgggc
     4381 gccttctcac ggatggcctg cgcgaccacg tcggagagcg ggaaaccctc gcccgcgtag
     4441 tggatcgccg gctcgagcaa cgaggccagc gggcgggtgc cgaagcgatc gagcagcatc
     4501 cgccagccgc gcgtcacgcc ggggaccgac accgagcccg gccccacgcc cggtaccgcg
     4561 gtgagcccgc gccggcccag ctcctccagc gtggcgcgtg cgcccgagcg gcccgcgccg
     4621 ctcaggaagt gcacgcgctt cgtggccgcc tcgtagtaga ggcagaagat gtcgccgccc
     4681 acgccgcaga agttgggctg ggtgaccgcg agcaccgcgt tggtggcgac ggccgcgtcc
     4741 accacgttgc cgcccgactt cagcacgtcc acgccggcca gcgaggcgag cggatggcac
     4801 gcggccacca tgccgcgccg gcccatgggc gcctgacgat gcgcgtaacc gatctggggc
     4861 atcctagccc tccgagaaga tgcgggtgat ctgatcctgg gggaacgtgg gcatggggcg
     4921 cgcgtggccc ccggtggcga ggctcacgcc ctgcgaggcc tcggtgacgc ggccgatcgg
     4981 cgcgcagtcc acgccggcgc cgcggcaggc cgcggccacg cccgcggcgt cggccggatc
     5041 caccgcgagc agcagcgagc ccgaggcgat ggtgccgagc gggtcgaggc caaaggcctc
     5101 gcagagcacg cggccctcgt gcagcaccgg gatgcgctcg cgatccacgc ggagccccac
     5161 gtcggcggcc tgggccagct cccagcacgc ggtggccacg ccaccttcgg tcgggtcgtg
     5221 catggcgtgc acgcgcgcgg cggcgcaggc cgcgcgggcc tcgggcacca cgctgatccc
     5281 gggattgcgc aggaaatcac gtgcgcgctt caccacgtcg gcggaaaccc cgcgccgctc
     5341 ggcctcggcc gcgcgctcgc gcgcgatgat cgccgcgccc tcgaggggta cgcccttggt
     5401 gaggagcagc acgtcgccgg ggcgcgcgcc gccggtggtg accaggcggt ccttctccac
     5461 ctcgcccagc atgcagccgg agacgatcgg gcgcggcagg ccggtcgtca cctcggtgtg
     5521 gccgccgacg agggccacgc cgagctccgc gcaggcctcg cgcagctcgg tgaacaggcg
     5581 ctcgacaccg agctcggtcg cgcgcccctc gggcagcagc acggtggcga gaaaccacaa
     5641 cggccgcgcg ccgcgcaccg cgagatcgtt ggcgttgacg tgcagggcgt accagccgag
     5701 ctcgtccgtc gcgaacgtga tcggatcggt ggtcgcgacc aggtaacgct cgccgatgtc
     5761 gagcaccgcc gcgtcctcgc cgacctgcgg ccccacgacc acacgcggat cgtcggaggc
     5821 ctgcgcgctc agcaagcgct ggagcagctg cggcggcagc ttgccgaccg agaggggtgg
     5881 catagcccac gtagtgtatc gcaataattt cggcctacct tgacgggccc gccgggacgg
     5941 gcgcctgcgc gccgcctacg cctggcggtg cgcgccggct actacggagg gtgaccgccc
     6001 cggctggccg acccggcctc gtgcccggac ttcaggctgg ccgattcgac ccgtggctgg
     6061 agagagagct gcccccggat tcacttctga tataactgtt ctaactgtct gaagatctat
     6121 agaaggttag aataacgggc ccgcggcgcg cagcgcggcc acgaccaggg gcttcggata
     6181 gagccccatg atcacgaccc cgaggacgca gagaaagatg cagaaggaga gcagcggggc
     6241 caccgggatc ggatcgcgcc gctcgggggc gtcgatgtac atgctcttgg cgatcaggag
     6301 gtagtagaag agggccacca ccgtcaggac cgcgcccacc agcactaacc agtaaagccc
     6361 ctgctccgcg gccgcccaga agacgtagag cttcgcccag aaaccggcta cgaacggaat
     6421 gcctcccagc gaaagcagga agagcagcat gcagagggcg aggatcgggg agcgctgggc
     6481 gagcccgcga taggccgtga taccctccga gccctcggcc tgggacacgg cctccacgac
     6541 caggaacgcg cccatgtttc caaatagata cgccaccagg tagaagagca tcatggcgac
     6601 gccattggcc gagacggcgg cgagcccgat cagcatgtag ccgatgtggg cgacccccga
     6661 gtaggccagc agccgcttga tgttctgctg cgggatcgcc atgaggttcc cggtcaccat
     6721 cgtcatgccc gcgagc
//
LOCUS       DASUFA010000066        13064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_816463, whole genome shotgun sequence.
ACCESSION   DASUFA010000066 DASUFA010000000
VERSION     DASUFA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13064
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816463"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VFX87_02465"
     CDS             <1..1132
                     /locus_tag="VFX87_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4993796.1"
                     /translation="GEPTGLLIDLAQDLIRGLVPPPSEERFDGAVEAVVGECLAKGLT
                     GAHEPGLDLAAVASYTRLIERGRFPFRVFAALSGKKAWGQYRERGRPESVGDGYLTVG
                     AIKLWLDGALGSRGAALHSPYCDDPGNTGLVLVPPEDVERLTREVSARGFHVWVHAIG
                     DRANTMVLDVFERVRAAGGPQSRLRVEHAQILTPADIPRFALLGVVPSMQPTHCTSDM
                     RWAGERLGPERLAGAYAWRSVLDTGAYLAGGSDFPVEDANPFHGLHAAVTRRPREGPD
                     PGWQPGQRMTRDEAVRSFTIWNARSVGREADQGSLEVGKRADLIALSDDVFTCAPPAI
                     ARLTPTLTMVGGVVAYAVDQDPGVSRAASRASSAGSTSDTSA"
     gene            complement(1060..1848)
                     /locus_tag="VFX87_02470"
     CDS             complement(1060..1848)
                     /locus_tag="VFX87_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX4993797.1"
                     /translation="MTCEEARDRLLDAQRGRLAPETRAALAAHLQTCAACAHEEAAEA
                     LLSEALESRLPQHGAPVALKRRLAAEWPAAAATTSGPWRRWRPYLVPAAAAAAVLVIV
                     LPLAYQRAAERAETRMVAEAVNDHLRILTSQHPLDIESGGIHQVKPWFEGRLDFAPVV
                     RFDGDRDFPLRGGAVGYYLDRKAAVFVYNRHLHTISLFVFRADGLPWPTRGLERVGGS
                     QARVTTSRGFHSVLWRDGELGYALVSDVDPAELARLAARLTPGS"
     gene            complement(1845..2387)
                     /locus_tag="VFX87_02475"
     CDS             complement(1845..2387)
                     /locus_tag="VFX87_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4993798.1"
                     /translation="MARWGRADTGGLGREALAYADTLYNLARYLTRSPTDAEDLVQET
                     YARALTAAHQYTPGTNLKAWLFRILRNTFLSQYRHERHNPMVGGLDTVAPTAQDAERA
                     QWLRGDLELDRLRKVVGEEIERALMSLSEEARTVILLDLEGLTEVEIALVVGCAVGTV
                     KSRLSRARASLRALLKDYAP"
     gene            2593..2922
                     /locus_tag="VFX87_02480"
     CDS             2593..2922
                     /locus_tag="VFX87_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007941931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX4993799.1"
                     /translation="MALAGTAVVALLLASPGPARAQQETGLRIEIKAHGYGIPELTVP
                     PGTTVTWVNHDDDPHTVTSTGNVFRSPGLDTDETYSYTFTRPGTYEYFCTLHPMMTGK
                     VVVRSGS"
     gene            2919..3866
                     /locus_tag="VFX87_02485"
     CDS             2919..3866
                     /locus_tag="VFX87_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4993800.1"
                     /translation="MSDQRGVDRRGFLQCMAWTGSGLLWSVAGGVLSSRAIGVGAAEA
                     QSAAASFSFAQISDTHIGFKGAANPDAMGTFEAAIARIKALDPRPAFLIHTGDITHGQ
                     KAGAFDVAREALKGTGIERTFYVPGEHDVFADGGAEYLNRFAAGRPGRGWQSFDYGGV
                     HFVGLVNVLSYKAGGLGSLGPEQLEWLERDVAGLASSTPVVVFAHVPLWTVYPQWGWV
                     TDDATQALAYLKRFGSVTVLNGHIHQIMQKVEGNVTFHTARSTAFPQPAPGAAAAPGP
                     MKVPVEELRGMLGVREVTYAPGRDRLAVVDASLASSAAG"
     gene            complement(3863..5203)
                     /locus_tag="VFX87_02490"
     CDS             complement(3863..5203)
                     /locus_tag="VFX87_02490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX4993801.1"
                     /translation="MLTVLLVGFFYWSWGSPLANLERPEESLERLVSREMDLHDVMDG
                     LPRWEKRLYELTGSEPETFEDAIARFDELDEDQRSARTDLDLVVLLAESGQLERAASI
                     IEALEGTDTAAAQYGRWLDAAYLEAPTPQDATLMVEEIRRELPRDWFTDTLARRIATR
                     VDDPALQTEAEASIEARGHAVLRRVRGLAGTGVGLFLLALALLWRLLRSPRGASVAEA
                     PLPPVWRGADGVGLFFRAAFAYLLMPAALVVLLPRTPPSTVAMGLLGGVPMIWWVRRY
                     LHARGDTLRETFGLRVPHGQALRVIGWGVVLLGLSMAGESLLSMGLTALGVTSHWADG
                     FLEDFLWGSPATVMAGTIDGVVWAPIFEELAFRGLLYPTLRLRLPPWPAALLSAALFG
                     VAHGYGLQGFAAVTWSGMIWALGYERTRSLLPGMLAHAASNLMATTSFLLLLRF"
     gene            complement(5272..5997)
                     /locus_tag="VFX87_02495"
     CDS             complement(5272..5997)
                     /locus_tag="VFX87_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX4993802.1"
                     /translation="MAHRLPLIGVSTSITVGASPERAYVNSAYLHAVQQAGGVPVPLP
                     PQLSAASLRQLGAELRGLLLTGGGDMDPALFGQAPHPSLYDVAPSRDTLETSALNLAL
                     GRGVPVLAVCRGLQVLNVALGGTLYQDVATEPGTQIPHSQKEPRDQPTHKVKITPGSR
                     LAETLGTDEIEVNSMHHQAIHRLGRGLTAVAWAPDQLVEGAEIRDPGRFVLGVQWHPE
                     ELVGQSAPARRLFSALVAAASKS"
     gene            6021..7790
                     /locus_tag="VFX87_02500"
     CDS             6021..7790
                     /locus_tag="VFX87_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX4993803.1"
                     /translation="MTLDSAQYQRSAAPAGSGPMTQVMGRKWMVAAGHPLAAQAAARV
                     LAEGGNAIDAGVAAGFMLGVVHPDMVSFAGVAPILVHLASTGETFEVSGVGPYPRRLT
                     ADLFRTKHAGDIPVGLLRTVVPASPDAWCVALERWGTRSFTDSVSSALECAEHGFPTS
                     AFSASIMANNAERYARFPTSAKIFLPGGKPHAAGQLFVQAELAATIKEMVAAEVRSRS
                     RGRAGAIRAARDAFYKGDIAKRIVEYHAREGGLMALEDLAEFQAEVAPALKTTFHDFE
                     VAACGFWCQGPVFLQMLNLIEPYDWPALGHNTPRALHILVEAMKLAFADREAYYGDPR
                     FVKVPADGLLSKAYADLRRGLIREDRAWPEMPPPGDPEGRAAVRNGPGVASKAGAPSH
                     AGTLDTSYVSVIDAEGNAFSATPSDPGGVDSPVIPGVGCVVSPRGSQGWLVPGHPSEV
                     VGGKRPRLTPAPSLVLRDGRAFMAFGTPGGDVQQQAMLQVLLNVVAYGMAPQQAVEAP
                     RLATRSFPDSFWPHTNSPGRLDVEARIAPETRAALAKLGHVIADWPDYDWRAGAVCAS
                     VVGKDGVRLGGADPRRGAHAIGW"
     gene            7979..8533
                     /locus_tag="VFX87_02505"
     CDS             7979..8533
                     /locus_tag="VFX87_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993804.1"
                     /translation="MELEPYTARPEIFEYLLDHPEFASHVTRALRAARYKIWREAGSL
                     WLDDGWGVKGTFALAHAEPGLRLMHAQGAYQSALPDIKGRAIVALAYKFQPSPQAGRQ
                     QVATVLTSFVQIDSAVIRTIGRVGGPLVQKKADREARQLLRVFSKVSRAIEENPADVY
                     AKVRERADVPKQELEEFRKLLRVP"
     gene            complement(8578..9930)
                     /locus_tag="VFX87_02510"
     CDS             complement(8578..9930)
                     /locus_tag="VFX87_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX4993805.1"
                     /translation="MSIHPSDGPAPLQPSQPAPPHLAELDRLGDQIAELSAHLEAATA
                     RLLALIREFDARGGWSNGFRSCAEWLAWRVGLDPGAARERVRVARALETLPALADALA
                     RGELSYAKVRALTRIATPETEARLLAVGRAGTAAHIESIVRGWRRLDRQAEAREATRQ
                     HAGRALHVHQDEDSTVVLHGRLTPEVGTLLLRALDAARETLYQRRRATETSTWPQQQA
                     DALALLAETALHHELDPGAPGERYQVVVHVDAAVLADPAKPGQSVLEEGPRVSAETSQ
                     RLACDASRVVMRHDADGRVVEVGARTRTIPPALRRALQHRDRSCRFPGCNVRIAEGHH
                     VRHWAHGGRRSSRTSRYSAAGITGRCTRRATRSSVAPPGPSSSDGLTAGRCLMYRRRR
                     PCPPIPRERSGHRTPRRDSICMRGQGSPSGSESGWTCAGRSTCYIRGPRENVETRKRE
                     "
     gene            10234..11028
                     /locus_tag="VFX87_02515"
     CDS             10234..11028
                     /locus_tag="VFX87_02515"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4993806.1"
                     /translation="MMASLNGRTVLITGAARGIGAATARRLAGDGARLVLADQDGAGV
                     EKLAAELKQVAVKADVTKPDDIARMVDEPYKRWGRLDVLFNNAGVIRVQPLFDVTEAE
                     WDRVMDVNLRAVFFVMQAVAKRMVKQDVMPGSELRGKLIQTASIASYRGGNHLMTPYS
                     ASKAGVVSLTRSAAQLLAPERVTSNCVCPGAVETPMWEQIDKEWGALEGLGQGEAWKR
                     RIRHIPLGRPERAEDVANVVAFLAGPDSDYMTGQAINVDGGIVMGS"
     gene            complement(11032..11250)
                     /locus_tag="VFX87_02520"
     CDS             complement(11032..11250)
                     /locus_tag="VFX87_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993807.1"
                     /translation="MRRLATALLAATLLTGCATTSRPSASRADQTAQGPRRCSPADPD
                     RWVWFCVVGQILYGAAAFFTPVNETTMR"
     gene            complement(11266..>13064)
                     /locus_tag="VFX87_02525"
     CDS             complement(11266..>13064)
                     /locus_tag="VFX87_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4993808.1"
                     /translation="LEGLKALIPPGSVRFSGFTVVKASDVTDQEVLSSIKRDLIDKES
                     IVSNARFAKLQEKLRTLFRRASLHLSLAAIEGERILVLNSGHQLDHGHGCIFASSVRG
                     KTKDYAGSIFEKAVVRGAPMFVEDLAAVSPRSPKEEMILQKGMRSMVVAPLHYQDQLI
                     GTLSLASPNPGDINALLGPRLQEVLPLFSVAVRRSVDELHSRVEGFIKEQFTAIHPVV
                     EWRFRKAVLETMDRHTAEGEPSREMEPIVFRDVHPLYAVSDIRGSSTHRAWAIQADLL
                     TQLGLARDVLRAAYEVRPLPILDQLTHRIDGCTAEIEVTLRSGDEMGVLGFLKTDLEP
                     LFGHLQTFGPAVHEKIDAYRAAVDPGIGLVYARRKEFDESVTRINDAVASYIDLEEQA
                     AQGMFPHFFEKQKTDGVDYTIYLGGALREDGVFDPLYLKNMRLWQLMVACGVALRVER
                     LKDRLPVRLDITSLILIQHAPLSIRFRADEKRFDVDGAYNVRYEIIKKRIDKATIRGT
                     TERLTQPGKIALVYSHPSEAAEWREYIEYLQRLGYLTSDVEELELDELQGAQGLRALR
                     VTVDLANRELDVPSTAAVTTLPRAEGERTPAA"
BASE COUNT         1845 a         4618 c         4745 g         1856 t
ORIGIN      
        1 cggcgagccg accgggctcc tgatcgatct ggcgcaggac ctgatccgcg gcctcgtgcc
       61 gccgccgtcc gaggagcgct tcgacggagc ggtggaggcg gtggtggggg agtgcctggc
      121 caaggggctc accggcgcgc acgagcccgg gctcgacctg gccgccgtcg cctcgtacac
      181 gcgcctgatc gagcgcggcc gcttcccgtt ccgggtcttc gcggcgctca gcggtaagaa
      241 ggcgtggggg cagtatcgcg agcgcggccg gcccgaatcg gtgggcgacg ggtacctgac
      301 ggtgggcgcg atcaagctct ggctcgacgg cgcgctcggc tcgcgcggcg ccgcgctgca
      361 ctcgccctac tgcgatgatc cggggaacac cgggttggtg ctggtgccgc ccgaggacgt
      421 ggagcggctc acccgcgagg tgagcgctcg gggcttccac gtgtgggtgc acgccatcgg
      481 cgatcgcgcc aacaccatgg tgctcgacgt gttcgagcgc gtgcgggcgg cgggcggccc
      541 gcagtcacgg ttgcgcgtag agcacgcgca gatcctcacg cccgccgaca tcccgcgctt
      601 cgcgctcctc ggcgtggtgc cgagcatgca gccgacgcat tgcacctccg acatgcgctg
      661 ggcgggagag cgcctcggcc ccgagcgcct ggccggcgcc tatgcctggc gctcggtgct
      721 cgacaccggc gcctacctcg cgggcggctc cgacttcccg gtggaggacg ccaacccctt
      781 ccacggcctc cacgccgcag tgacgcggcg gccgcgcgag ggccccgatc cgggctggca
      841 gcccgggcag cgcatgacgc gggacgaggc ggtgcggtcg ttcacgatct ggaacgcccg
      901 ctccgtcggc cgcgaggccg atcaaggctc gctcgaggtg ggcaagcgcg ccgacctcat
      961 cgcgctctcg gacgatgtgt tcacctgcgc gccgccggcc atcgcgcgcc tcacgcccac
     1021 gctcaccatg gtgggcgggg tcgtggcgta cgccgtcgat caggatcccg gcgtgagccg
     1081 cgccgcgagc cgcgcaagct ccgccggatc gacgtcggac accagcgcgt agcccagctc
     1141 gccgtcccgc cacagcaccg agtggaaccc gcgagaggtc gtgacgcggg cctggcttcc
     1201 gcccacacgc tccagccccc gggtcggcca cgggaggcca tcggcgcgga agacgaagag
     1261 cgagatcgtg tgcaggtgcc ggttgtagac gaagacggcg gccttgcggt ccaggtagta
     1321 gccgaccgcg ccgccccgca gcgggaagtc ccgatcgccg tcgaagcgta cgaccggcgc
     1381 gaagtcgaga cggccctcga accacggctt gacctgatgg atgccgccgc tctcgatgtc
     1441 gagcgggtgc tgactcgtca ggatccgcag gtgatcgttg acggcctcgg cgaccatccg
     1501 tgtctcggcg cgctcggccg cccgctggta ggcgagcggc agcacgatca cgagcaccgc
     1561 ggccgccgcc gcagccggga cgaggtaggg ccgccagcgc cgccacggcc ccgaggtggt
     1621 cgccgccgcg gccggccact ccgcggccag ccggcgcttg agagccaccg gggctccgtg
     1681 ctgggggagc cggctctcca gcgcctcgct caggagcgcc tccgccgcct cctcgtgcgc
     1741 gcacgccgcg cacgtctgca ggtgcgccgc gagcgcggcg cgcgtctccg gggcgagccg
     1801 gccgcgctgg gcgtccagga gccggtcccg ggcctcctcg caggtcatgg cgcgtagtcc
     1861 ttgagcagcg cccgcagcga cgcgcgcgcc cgcgacagcc gggacttgac ggttccgacc
     1921 gcgcagccca cgaccagggc gatctcgacc tcggtcagcc cctcgagatc caacaggatc
     1981 acggtacgcg cctcctcgct gagactcatg agcgcgcgct cgatctcctc gccgaccacc
     2041 ttccgcaggc ggtccagctc gagatcgccg cgaagccatt gggctcgctc ggcgtcctgc
     2101 gcggtcggcg ccaccgtatc gagcccgccc accatggggt tgtgtcgctc gtgccggtac
     2161 tggctgagga acgtgttgcg caggatccgg aagagccagg ccttgagatt ggtgcccggg
     2221 gtgtactggt gcgccgcggt gagggcacgc gcgtaggtct cctgcacgag gtcttccgca
     2281 tcggtcgggc tgcgggtgag gtaccgggcg aggttgtaga gcgtgtccgc gtaggccagc
     2341 gcctcgcgcc cgagcccgcc ggtgtcggct cgcccccacc tcgccatgtc gtcatcatag
     2401 gcctcgcccc agtcactctg ccatcggcgg ctcctcgcca cggccaggcc cgctcgccct
     2461 tcgacgagcg caacgcaggg ccggccggat cgttccatcc gagaattttt tttggccatg
     2521 gaacgacggg agcggggcgg cgttggcatg ggcgaaggag gaccgacaat gaatgcacga
     2581 gggtggacaa ggatggcctt ggcgggaacc gcggtggtgg cgctgctgct ggcctcaccg
     2641 ggcccggcgc gggcgcagca ggagacgggg ctccgcatcg agatcaaggc gcacggctac
     2701 ggcatccccg agctgaccgt cccgccgggc accaccgtca cctgggtcaa ccacgacgac
     2761 gatccccaca ccgtgacgtc caccgggaac gtcttccggt cgccgggtct cgataccgac
     2821 gagacctact cctacacgtt cacgcgaccg ggcacctacg agtacttctg caccttgcac
     2881 ccgatgatga cgggcaaggt cgtggtgagg agcggatcat gagcgaccag cgtggtgtcg
     2941 accggcgggg ctttctccag tgcatggcgt ggacgggatc gggcctgctg tggtccgtcg
     3001 cgggcggggt gctctcgtcc cgggcgatcg gcgtgggcgc ggcggaggcg cagtccgcag
     3061 cggccagctt cagcttcgcc cagatcagcg atactcacat cggcttcaag ggcgccgcga
     3121 acccggacgc gatgggcacc ttcgaggcgg cgatcgcccg catcaaggcg ctcgatccca
     3181 ggcccgcctt cctgatccac accggcgaca tcacgcacgg gcagaaggcg ggcgcattcg
     3241 acgtggcccg cgaggcgctc aagggcaccg gcatcgagcg caccttctac gtgccgggcg
     3301 agcacgacgt gttcgccgac ggcggcgccg agtacttgaa tcgcttcgcg gcgggccggc
     3361 cgggtcgcgg gtggcagagc ttcgactacg gcggcgttca cttcgtcggc ctcgtcaacg
     3421 tgctcagcta caaggcgggc gggctcggca gcctggggcc cgagcagctc gagtggctgg
     3481 agcgcgacgt cgcgggcctc gcgagcagca cgcccgtggt ggtcttcgcc cacgtcccgc
     3541 tctggacggt gtacccgcaa tggggctggg tgaccgacga cgccacgcag gccctcgcct
     3601 atctcaagcg cttcggctcc gtcaccgtgc tgaacggcca catccaccag atcatgcaga
     3661 aggtggaggg caacgtcacc ttccacaccg cacgctccac cgcctttccg cagcccgccc
     3721 ccggcgccgc ggccgccccg ggtcccatga aggtgccggt ggaggagctg cggggcatgc
     3781 tgggagtccg tgaggtcacc tacgcgccgg gccgggaccg gctggcggtg gtggacgcct
     3841 cgctggcgtc gagcgcggcg ggctagaagc gcagcagcag cagaaagctc gtcgtcgcca
     3901 tcaggttgct ggccgcgtgg gccagcatgc cgggcagcag gctgcgcgtg cgctcgtagc
     3961 ccagcgccca gatcatcccg ctccacgtga ccgccgcgaa gccctgcagc ccgtagccgt
     4021 gggccacgcc gaagagcgcc gcgctcagca gggcggccgg ccagggcggc agccggaggc
     4081 gcagggtggg atagagcagg ccgcggaagg ccagctcctc gaagatgggc gcccagacca
     4141 cgccgtcgat ggtgccggcc atcacggtgg ccggtgagcc ccagaggaaa tcctcgagga
     4201 agccgtccgc ccagtgcgac gtgacgccga gggcggtgag gcccatggag agcaggctct
     4261 cgcccgccat cgagaggccg aggagcacga cgccccagcc gatgacgcgg agggcctgcc
     4321 cgtgcggcac gcgcaggccg aaggtctcgc ggagtgtgtc gccgcgggcg tgcaggtagc
     4381 ggcggaccca ccagatcatg ggcacgccgc ccagcagccc catggccacg gtgctggggg
     4441 gcgtgcgcgg cagcagcacg accagcgccg cgggcatgag caggtaggcg aaggcggcac
     4501 ggaagaagag gccgaccccg tcggcgccgc gccagaccgg cggcagcggc gcctccgcca
     4561 cgctcgcgcc gcgcggggag cggagcaggc gccagaggag cgccagcgcc agaaggaaca
     4621 gcccgacgcc cgtgccggcg agcccccgta cccgccggag caccgcgtgt ccgcgggcct
     4681 cgatgctcgc ctcggcctcg gtctgcagcg ccgggtcgtc cacccgcgtg gcgatgcggc
     4741 gcgccagcgt gtcggtgaac cagtcgcgcg gcagctcgcg gcggatctcc tcgaccatca
     4801 gggtcgcgtc ctgcggtgtc ggcgcctcga gataggcggc gtcgagccag cggccgtact
     4861 gcgcggctgc ggtgtccgtg ccctccaggg cctcgatgat ggaggcggcc cgctcgagct
     4921 ggccgctctc ggccagcagc accacgaggt ccagatcggt gcgcgccgag cgctggtcct
     4981 cgtccagctc gtcgaagcgg gcgatggcgt cctcgaacgt ctccggctcc gagccggtga
     5041 gctcgtagag cctcttctcc caccggggta acccgtccat cacgtcgtgc agatccatct
     5101 cgcgggagac caggcgctcg agggactcct cgggcctttc caggttggcg agcggcgagc
     5161 cccagctcca gtagaagaag ccgaccagca ggacggtgag cacggcgtag agcgccgtgg
     5221 cccagggaga gaagtagcgg gtggcgggag cggacccgtc tccgggcagc gtcaggattt
     5281 gcttgccgcg gccacgagcg cggagaagag gcgccgggcc ggcgccgact ggcccaccag
     5341 ctcctccgga tgccactgca cgccgagcac gaagcgcccg ggatcgcgga tctccgcgcc
     5401 ctcgaccagc tgatcgggcg cccacgccac cgcggtcagg ccgcggccga ggcggtggat
     5461 cgcctggtgg tgcatgctgt tcacctcgat ctcgtcggtg cccagcgtct cggccagccg
     5521 gctgcccggg gtgatcttca ccttgtgggt gggttgatcc cgcggctcct tctggctgtg
     5581 cgggatctgg gtgccgggct cggtggccac gtcctggtag agggtgccgc cgagcgccac
     5641 gttcagcacc tggaggccgc ggcacaccgc gagcacgggc acgccgcgcc cgagcgccag
     5701 gttcagcgcc gacgtctcca gcgtgtcgcg cgacggggcg acgtcgtaga gcgacggatg
     5761 cggcgcctgg ccgaagagag ccggatccat gtcgccgccg cccgtgagca ggagcccccg
     5821 gagctccgcg ccgagctgcc ggagcgatgc cgcggagagc tgcggcggca gcggcaccgg
     5881 gacaccgccc gcctgctgga ccgcgtgcag gtacgcggaa ttgacgtaag cgcgctcggg
     5941 acttgcgccg accgtgatcg acgtgctgac gccaatgagc ggcaagcggt gagccatgac
     6001 ggtatgttag catacgcgcc atgacactcg actccgcgca gtaccagcgt agcgctgcgc
     6061 cggcgggctc gggcccgatg acgcaggtga tggggcggaa gtggatggtg gcggccgggc
     6121 atccgctcgc ggcgcaggcg gccgcgcgcg tgctggccga gggcggcaac gcgatcgacg
     6181 cgggagtagc cgccgggttc atgttgggag tcgtccatcc cgacatggtg agcttcgcgg
     6241 gcgtggcgcc gatcctggtg cacctggcct cgaccggcga gaccttcgag gtctcgggcg
     6301 tggggcccta tccgcggcgc ctgaccgccg acctcttccg caccaagcac gcgggcgaca
     6361 tcccggtcgg cctgctgcgc acggtggtgc ccgcctcgcc cgacgcctgg tgcgtggcgc
     6421 tcgagcgctg gggcacgcgg tcgttcaccg attcggtgtc ctccgcgctc gagtgcgcgg
     6481 agcacggctt ccccacctcg gcgttctcgg cctcgatcat ggccaacaac gccgagcgct
     6541 acgcgcgctt ccccacctcc gccaagatct tcctgccggg cggcaagccg cacgccgccg
     6601 gtcagctctt cgtgcaggcg gagctcgccg cgacgatcaa ggagatggtg gccgccgagg
     6661 tgcgctcgcg cagccgcggc cgcgccggcg ccatccgcgc cgcgcgcgac gccttctaca
     6721 agggcgacat cgccaagcgc atcgtggagt accacgcccg tgagggcggc ctgatggcgc
     6781 tcgaggatct ggccgagttc caggccgagg tcgcgcccgc gctcaagacc actttccacg
     6841 attttgaagt cgcggcctgc ggcttctggt gccagggacc ggtatttcta caaatgctga
     6901 acctgatcga gccctacgac tggcccgcgc tcggccacaa tacgccgcgc gcgctccaca
     6961 tcctggtgga ggcgatgaag ctcgccttcg cggaccgcga ggcctactac ggcgatccgc
     7021 gcttcgtgaa ggtgccggcg gacgggctgc tctcgaaggc ctacgcggac ctgcgccgcg
     7081 gcctgatccg cgaggaccgt gcctggcccg agatgccgcc gccgggcgac ccggagggca
     7141 gggccgcggt gcgcaacggg cccggcgtcg cctccaaggc aggcgcgccc tcgcacgcgg
     7201 gcacgctcga tacgtcgtac gtctcggtga tcgacgcgga gggcaatgcc ttctccgcca
     7261 cgcccagcga cccgggcggg gtggactcgc cggtgatccc gggcgtgggc tgcgtggtct
     7321 cgccgcgggg ctcgcagggc tggctcgtgc ccggtcatcc gagcgaggtc gtgggcggta
     7381 agcggccgcg gctcacgccg gcgccgtctc tcgtgctgcg cgacggccgg gccttcatgg
     7441 ccttcggcac gccgggcggc gacgtgcagc agcaggccat gctgcaggtg ctgctcaacg
     7501 tggtggccta cggcatggcg ccccagcagg cggtggaggc cccgcggctg gccacgcgca
     7561 gcttccccga ttccttctgg ccccacacca actcgccggg tcgtctcgac gtcgaggccc
     7621 gcatcgctcc cgagacgcgc gcggcgctgg cgaagctggg gcacgtcatc gctgactggc
     7681 ccgactacga ctggcgcgcg ggcgcggtct gcgcctcggt ggtgggcaag gacggcgtgc
     7741 gcctgggcgg agccgaccca cgccgcggcg cgcacgccat cggctggtag ccggcgcgcg
     7801 cccgcgctcg ctgtcccgcc gccgcttcgc ggttgtcgcg ctgctgagtc tctgcgcgct
     7861 ggtcgggggc gcgggtccgg tcgcggccca gccgaccctg cacctcccgc cgcagcttcc
     7921 tccgaaggat cggcagcggt tcgaggacat cgtcaagcgg gccttcgcct ccacccggat
     7981 ggagctcgag ccctacactg cccgccccga gatcttcgag tacctgctcg atcacccgga
     8041 gttcgccagc catgtcaccc gcgcgctcag ggccgcccgc tacaagatct ggcgcgaggc
     8101 cggctcgctc tggctcgacg acggctgggg cgtgaagggg acgttcgctc tcgcccacgc
     8161 cgagccgggc ctgcggctga tgcacgccca gggcgcctac cagtcggccc tgcccgacat
     8221 caagggccgc gcgatcgtgg cgctggccta caagttccag cccagcccgc aggccggccg
     8281 ccaacaggtg gccaccgtgc tcacctcgtt cgtgcagatc gacagcgcgg tcatccgcac
     8341 catcggccgc gtgggcggcc ccctcgtgca gaagaaggcc gaccgcgagg cgcgccagct
     8401 cctgcgcgtg ttctccaagg tgagccgcgc gatcgaggag aatcccgccg acgtctacgc
     8461 caaggtgcgc gagcgggcgg acgtgcccaa gcaggagctg gaggagtttc ggaagctgct
     8521 gcgcgtgccg taggggagat ggtgcgcgcg ttggggcagg atcagctccc ggccgagtca
     8581 ctcgcgctta cgggtctcga cgttctcgcg tggcccgcgg atgtagcacg tcgatcgccc
     8641 agcgcacgtc cagccgctct ccgaaccact gggcgagccc tgtccgcgca tgcagattga
     8701 gtccctgcgc ggcgttctgt gcccggagcg ctcccgcggg atcggcgggc acggccgccg
     8761 gcgccggtac atcaggcagc ggccggccgt caggccgtcg gaactggagg gccccggcgg
     8821 cgccacgctc gacctggtag ccctcctcgt gcaccgcccg gtgatgccgg cggcagagta
     8881 acgcgaggtt cgagagcttc gtcggccccc gtgggcccag tgccggacgt gatgcccctc
     8941 ggcgatccgg acattgcagc ccgggaagcg gcagctccta tcccgatgtt gcagtgcccg
     9001 ccgcagcgcc ggcggaatcg ttcgcgtcct ggcgccgacc tcgaccaccc ggccgtccgc
     9061 atcatgccgc atcaccaccc ggcttgcgtc gcacgccagt cgctgcgacg tttccgcgga
     9121 aacgcgcgga ccctcctcca gcacggattg cccgggcttg gccggatccg cgaggaccgc
     9181 ggcgtccacg tgcaccacga cctgatagcg ctccccgggg gctcccggat cgagctcgtg
     9241 atgcagcgcc gtctcggcca gcagcgccag ggcatcggcc tgctgctgag gccaggtcga
     9301 cgtctcggtg gcgcggcgcc gctggtacag cgtctcgcgg gccgcgtcga gggcccgcag
     9361 gagcagcgtg cccacctccg gcgtcagccg cccgtgcagg accacagtgc tgtcctcgtc
     9421 ctgatggacg tgcagcgccc ggcccgcatg ctggcgtgtg gcctcacgcg cttcggcctg
     9481 ccggtccagt cggcgccagc cccggacgat gctctcgatg tgcgcggccg tgccggcgcg
     9541 ccccactgcg agtagccggg cttcggtctc cggggtcgcg atgcgggtca gcgcgcggac
     9601 cttggcatag gagagctccc cacgggccag tgcgtcggcc agggcgggta gcgtctcgag
     9661 ggcacgggcc acgcgaacgc gctcgcgggc ggcgcccggg tcaagcccaa cgcgccacgc
     9721 gagccactcg gcgcacgagc ggaacccgtt gctccagccc ccgcgagcgt cgaactcacg
     9781 gatcagcgcc agcaggcggg ccgttgccgc ctcgaggtga gccgagagct cagcgatctg
     9841 gtcgcccagg cgatccagct ccgcgaggtg cggcggggcg ggctgcgacg gctgcaacgg
     9901 ggcgggacca tcggacgggt gtatcgacat ggcgagtctc ccttcttgct gggccgactc
     9961 tacaccccga ttttgcaccg gtctgaacgc ctccaggacc ggcgaatgga gggtttgcca
    10021 tgatcgctcc tctgccgcga cgccaacgcg cagcgcgtcc tgaatgcgcg ccgctcagtc
    10081 ggcacttcct cgctcgatcg agcgcgaatt gtgaccgctc tccgtaactc gtcaagcgaa
    10141 aagtgcggat ggtcatagtg aaggtcgtgc gccgccgagt cgttgtagtg tatccagtcg
    10201 gatacgcagt gactcgatga acggggagga gccatgatgg catcgctcaa tggacgcaca
    10261 gtgctgatca ccggcgcggc ccgcggcatc ggcgccgcca ccgcccgccg cctcgccggc
    10321 gacggagccc ggctggtcct cgccgaccag gacggcgccg gcgtggagaa gctcgccgcc
    10381 gagctcaagc aggtggcggt gaaggccgac gtcaccaagc ccgatgacat cgcgcgcatg
    10441 gtggacgagc cctacaagcg ctggggccgg ctcgacgtgc tcttcaacaa cgccggcgtc
    10501 atccgggtgc agcccctgtt cgacgtcacc gaggcggagt gggaccgcgt catggacgtg
    10561 aacctgcgcg cggtgttctt cgtgatgcag gcggtggcga agcggatggt gaagcaggac
    10621 gtgatgccgg ggtcggagct gcgcggcaag ctgatccaga ccgcgtccat cgcgtcgtac
    10681 cggggcggca accacctcat gacgccgtac tcggcgtcca aggcgggagt ggtgagcctg
    10741 acgcggtccg cggcgcagct cctggcgccg gagcgggtca cctccaactg cgtgtgcccg
    10801 ggggcggtcg agacgccgat gtgggagcag atcgacaagg agtggggcgc gctcgagggg
    10861 ctggggcagg gggaggcgtg gaagcggcgg atccggcaca tccccctggg gcggccggaa
    10921 cgggcggagg atgtcgccaa cgtggtggcg ttcctcgccg ggccggattc ggactacatg
    10981 accgggcagg cgatcaacgt ggacggcggc atcgtgatgg ggagctgatg gttaccgcat
    11041 cgtcgtctcg ttgaccggcg tgaagaacgc ggcggcgccg tagaggatct ggccgaccac
    11101 gcagaaccag acccagcggt cgggatcggc gggggagcag cgccgggggc cctgcgcggt
    11161 ctgatccgcg cgggaggccg acggccgcga cgtggtggcg caccccgtca gcagggtcgc
    11221 ggcgaggagc gcggtggcca ggcgacgcat ggccccggcc ggatgctacg ccgccggggt
    11281 ccgctcgcct tcggcgcggg gcagggtggt caccgcggcg gtggacggta cgtccagctc
    11341 gcggttggcc agatccaccg tcacccgcag ggcgcgcagg ccctgggcgc cctgcagctc
    11401 gtccagctcg agctcctcga cgtcgctggt gaggtagccc aggcgctgga ggtactcgat
    11461 gtactcacgc cactccgcgg cctcgctcgg gtgcgagtac acgagcgcga tcttgccggg
    11521 ctgggtcagc cgctcggtgg tgccgcggat cgtggccttg tcgatccgtt tcttgatgat
    11581 ctcgtagcgg acgttgtagg cgccgtccac gtcgaagcgc ttctcgtcgg cgcggaagcg
    11641 gatcgagagc ggggcgtgct ggatcaggat caggctggtg atgtcgagac gcaccggcag
    11701 ccggtccttc agccgctcca cccgcagggc cacgccgcag gccaccatga gctgccagag
    11761 gcgcatgttc ttgagataga gcggatcgaa gacgccgtcc tcgcgcaggg ctccccccag
    11821 gtagatcgtg tagtccaccc cgtccgtctt ctgcttctcg aagaagtgcg ggaacatgcc
    11881 ctgggccgcc tgctcctcca ggtcgatgta ggaggccacc gcgtcgttga tccgggtcac
    11941 gctctcgtcg aactccttgc gccgcgcgta gaccagcccg atgcccgggt ccaccgcggc
    12001 ccggtaggcg tcgatcttct cgtgcaccgc gggcccgaag gtctgcaggt ggccgaagag
    12061 cggctcgagg tccgtcttga ggaagccgag cactcccatc tcgtcgcccg agcgcagcgt
    12121 cacctcgatc tcggcggtgc agccgtcgat gcgatgggtg agctggtcga ggatgggcag
    12181 cggccgcacc tcgtacgcgg cgcgcagcac gtcgcgggcg aggccgagct gggtgagcag
    12241 gtcggcctgg atggcccacg cacggtgggt ggaggagccg cggatgtccg acaccgcgta
    12301 caacgggtgg acgtcgcgga acacgatcgg ctccatctcg cggctgggct cgccctccgc
    12361 ggtatgccgg tccatcgtct ccagcaccgc cttgcggaac cgccactcca cgaccgggtg
    12421 gatcgcggtg aactgctcct tgatgaagcc ctccacccgc gagtgcagct cgtcgacgct
    12481 gcgcctcacc gccaccgaga agagcggcag cacctcctgc aggcgcggcc ccaggagcgc
    12541 gttgatgtcg cccggattcg gggaggcgag gctcagcgtg ccgatgagct ggtcctggta
    12601 gtgcagggga gccaccacca tgctgcgcat gcccttctgc aggatcatct cctccttggg
    12661 cgagcgcggg ctcaccgcgg ccagatcctc gacgaacatc ggcgcgccgc gcacgaccgc
    12721 cttctcgaag atggagcccg cgtagtcctt cgtctttccg cgcaccgagc tggcgaagat
    12781 gcagccgtgc ccgtggtcga gctggtgccc gctgttgagc accaggatgc gctcgccctc
    12841 gatcgcggcc aggctcaggt ggaggctcgc ccggcggaac agggtgcgca gcttctcctg
    12901 gagcttcgcg aagcgcgcgt tcgagacgat ggactccttg tcgatcaggt cccgcttgat
    12961 cgaggagagc acctcttggt ccgtcacgtc ggaggccttc accaccgtga agccggagaa
    13021 gcgaacgctg ccgggcggga tgagggcctt gagcccctcc aggt
//
LOCUS       DASUFA010000067         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_702420, whole genome shotgun sequence.
ACCESSION   DASUFA010000067 DASUFA010000000
VERSION     DASUFA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702420"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="lptD"
                     /locus_tag="VFX87_02530"
     CDS             <1..900
                     /gene="lptD"
                     /locus_tag="VFX87_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX4993809.1"
                     /translation="PFAGGRLTYYDERVVDQRITRVGALSVEESVYDPRVRRQVEWGV
                     EAESRISRVYTSDGGGGVAAYQHVIEPRVRIIQIRGIDQKDYPQYDPGASTATGIDPG
                     YERRVGIDRLGKANEITYYLTNALNAKTVSGPDQQPVRWEMVRFTLSQTFKMDPVSEP
                     LKDLYGDLTVSPNQRIGFHADARYNVYDLGLREANADIRLTYPRFAVAVGPRFNEQGN
                     SRYAKAEALVKVLSNVDVRGSTAWDVLRGQAIENRVGIDWRFSCWSVMAEYVNRHGDE
                     NEFRISVNLLGLGQVGTSARSGF"
     gene            900..2003
                     /locus_tag="VFX87_02535"
     CDS             900..2003
                     /locus_tag="VFX87_02535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX4993810.1"
                     /translation="MIPQIDLKRQHEALRVELMDAAARVLDSCRFVLGPEGQGLEREL
                     ADLCGARHGVGTGSGTDALLLALKAVGVGPGDEVITSAFSFVASATTIVMAGATPIYV
                     DIEPDTFNLDPARVERAITSRTRAILPVYLYGQVAAMDALGDLARTRGLVIVADAAQA
                     VGASYAGRPVAAWADATTLSFYPTKNLGACGDGGMVLTTRDDVAERVRLLRDHGAPRR
                     YTHIELGACSRLDELQAALLRVKLKRLAGWNERRRAIARHYDTALAGLPIVLPVERSP
                     ARHIYHQYTVRSAKREALAAALAGLGVGTSIHYPSTIPAQPLFSQPNADRDFPVATQA
                     AAEVLCLPCFPELLDPEVDQVVDAIKRALAQVM"
     gene            2005..>2844
                     /locus_tag="VFX87_02540"
     CDS             2005..>2844
                     /locus_tag="VFX87_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX4993811.1"
                     /translation="MTSLGALGSRLEERLKSRSATVGVVGLGYVGLPLAVAFARAGFQ
                     VIGVDADPARTTRLAGGMSPVEDVPAAELAPLVQSGRLAIHSAPDALGEADAIVICVP
                     TPLGKSKEPDISFIVAAADGVARVLRPGTLVVLESTTYPGTTDEVLLPRMSRGGLRVG
                     EEFFLGFSPERIDPGNQTWRLSRIPKVVGGVTPVCRHLIETLYRQIIDRVVPVSSTQV
                     AEMVKLFENTFRSVNIALVNELAIMCRRLGMSVWEVIGAAATKPFGFMAFSPGPGIGG
                     HCLP"
BASE COUNT          407 a          991 c         1014 g          432 t
ORIGIN      
        1 ccgttcgcgg gcggccggct gacctactac gacgagcgtg tggtggacca gcggatcacc
       61 cgcgtggggg ccctctcggt ggaggagtcg gtgtacgatc cgcgcgtccg ccgccaggtg
      121 gagtgggggg tggaggcgga gagccgcatc tcccgcgtct acaccagcga cggcggaggc
      181 ggggtggcgg cctaccagca cgtgatcgag ccccgggtgc ggatcatcca gatccgcgga
      241 atcgaccaga aggactatcc tcagtacgac ccgggcgcga gcaccgcgac cggcatcgat
      301 cccggttacg agcggcgagt cggcatcgat cggctcggca aggccaacga gatcacctac
      361 tacctgacca acgcgctgaa cgccaagacg gtgtcgggtc cggaccagca gccggtgcgc
      421 tgggagatgg ttcgcttcac cctgagccag accttcaaga tggacccggt ctccgagccc
      481 ttgaaggatc tctacgggga tctcacggtc agccccaacc aaaggatcgg cttccacgcc
      541 gatgcccggt acaatgtcta tgatctgggc ctgcgagagg ccaacgcgga catccgcctc
      601 acgtatccgc gcttcgcggt ggcggtgggt ccccgtttca acgagcaggg caatagccgg
      661 tacgcgaagg cggaggcgct ggtgaaggtg ctgagcaacg tggacgtccg cggttccacc
      721 gcctgggacg tcctgagagg gcaggccatc gagaaccggg tgggtatcga ctggcgcttc
      781 agctgctggt cggtcatggc cgaatacgtg aaccgtcacg gcgacgagaa cgagttccgc
      841 atctcggtca acctcctcgg attgggccag gtcggcacct cggcccgctc gggcttctga
      901 tgattcccca gatcgacctc aagcgtcagc acgaggccct gcgcgtcgag ctgatggacg
      961 cggccgcgcg cgtgctcgac tcctgccggt tcgtcctggg gcccgaaggg caggggctcg
     1021 agcgcgagct ggccgatctc tgcggggcgc gccacggggt ggggacggga tcaggtaccg
     1081 acgccctgtt gctggcgctc aaggcggtcg gggtcgggcc gggcgacgag gtcatcacgt
     1141 ccgcgttctc cttcgtcgcc tccgccacca ccatcgtgat ggccggcgcc accccgatct
     1201 acgtggacat cgagccggac acgttcaatc tggatccggc ccgagtggag cgggcgatca
     1261 cgtcgcggac ccgtgccatc ctcccggtct acctctacgg gcaggtggcg gcgatggacg
     1321 ccctgggcga cttggcccgg acgcgcggcc tcgtgatcgt ggccgacgcg gcgcaggcgg
     1381 tgggcgcttc ctatgcgggc cgtccggtcg ccgcgtgggc cgacgccacc acgctgtcgt
     1441 tctacccgac caagaacctg ggggcctgcg gcgacggcgg catggtgctg accacgcggg
     1501 acgacgtggc cgagcgcgtc cggctgctcc gcgaccacgg cgccccccgg cggtacacgc
     1561 acatcgagct gggcgcctgc agccggctcg acgagctgca ggccgcgctg ctccgcgtga
     1621 agctcaagcg cctggccggg tggaacgagc ggcgccgcgc gatcgcccgg cactacgaca
     1681 ccgcgctcgc cggcctgcca atcgtgctgc cggtggagcg gtcgcccgcg cgacacatct
     1741 accaccagta cacggtgcgg tcggccaagc gggaagcgct cgcggcggcc ctggccgggc
     1801 tgggcgtggg gacctcgatc cactacccga gcacgatccc cgcgcagccg ctcttctcgc
     1861 agccgaacgc ggaccgcgac ttcccggtgg ccacccaggc cgccgccgag gtgctgtgcc
     1921 tgccgtgctt ccccgagctc ctggatccgg aggtggacca ggtggtggac gcgatcaagc
     1981 gggcgctcgc ccaggtgatg tgaggtgacg tcgctcggcg cgctcgggtc ccggctcgag
     2041 gagcgcctga agagccgctc cgccacggtg ggcgtggtgg gcctggggta cgtcgggctg
     2101 ccgctcgcgg tggccttcgc gcgggccggc tttcaggtga tcggggtgga cgccgacccc
     2161 gcccgcacca cgcggctcgc gggcggcatg agcccggtgg aggacgtgcc cgccgcggag
     2221 ctcgccccgc tggtgcagag cggacgcctc gcgatccact ccgcgccgga cgcgctgggt
     2281 gaggcggacg ccatcgtgat ctgcgtgccc actccgctcg ggaagtccaa ggagcccgac
     2341 atctccttca tcgtggcggc ggccgacggc gtggcccgcg tgctgcggcc cggcaccctg
     2401 gtggtgctcg agtcgaccac gtatcccggc accaccgacg aggtgctgct cccgcgcatg
     2461 agccgcggcg ggctccgggt gggtgaggag ttcttcctgg ggttctcgcc cgagcgtatc
     2521 gatcccggca accagacctg gcggctctcc cgcattccca aggtggtggg cggcgtcacc
     2581 cccgtgtgcc gccatctgat cgagaccctc taccgccaga tcatcgaccg cgtcgtcccg
     2641 gtatcctcca cgcaggtcgc ggagatggtg aagctgttcg agaacacctt ccgcagcgtc
     2701 aacatcgcgc tcgtcaacga gctggccatc atgtgccgcc ggctcggcat gtcggtctgg
     2761 gaggtgatcg gcgccgccgc caccaagccc ttcgggttca tggcgttctc cccggggccg
     2821 ggcatcggcg gccactgcct gccg
//
LOCUS       DASUFA010000068        13630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_816859, whole genome shotgun sequence.
ACCESSION   DASUFA010000068 DASUFA010000000
VERSION     DASUFA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13630
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816859"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /gene="hisH"
                     /locus_tag="VFX87_02545"
     CDS             complement(<1..173)
                     /gene="hisH"
                     /locus_tag="VFX87_02545"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838998.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX4993812.1"
                     /translation="MIAIVDYGRGNLGSVEKAFARIGMPAVVTQDPKVVDDAEAVVLP
                     GDGAFHDAMGSLQA"
     gene            complement(285..872)
                     /gene="hisB"
                     /locus_tag="VFX87_02550"
     CDS             complement(285..872)
                     /gene="hisB"
                     /locus_tag="VFX87_02550"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX4993813.1"
                     /translation="MPRQSRIERNTKETQIVLTLNLDGTGAAKVDTGIPFFNHMLEAW
                     SKHGLMDLHVDAKGDLDVDLHHTVEDVGICLGKALREAVGDKKGIVRYGASFLPMDEA
                     LLYAAVDISGRPYLVFNVPLQRTRIANFDLDLLKDFFRAVAVNAEITLHLNMHYGENL
                     HHIAEATFKAAGRALAEATRLNPRITGILSTKGSL"
     gene            complement(902..2212)
                     /gene="hisD"
                     /locus_tag="VFX87_02555"
     CDS             complement(902..2212)
                     /gene="hisD"
                     /locus_tag="VFX87_02555"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX4993814.1"
                     /translation="MIRKLETSGLGVAGVIRALERPPGSVDPEISRRVAEIVAAVREK
                     GDAALLDFTRRFDRVTLTAPELAVRPEEYAAAERAVGEATLRSLRYAAERVERFHREC
                     APRSWRMKDDHGSRLGQEVRPLDRVAVYVPGGRAAYPSTVLMTVVPAVVAGVREIVLV
                     SPPSADRSLNAAVLAAARIAGVTEAYRVGGAQAIAALAYGTETIRRVDKIVGPGNIYV
                     ALAKAQVFGDVGIDMVAGPSEVLVVADDTADAAFVAADLLAQAEHDPMARAVLLTPSD
                     ALRDAVAAELGRQLAGLPRREIAGAALASHGALVLTASLEEAVDLANGLAPEHLELQV
                     ADPEGLLVRVRHAGAVFLGRHTPEVVGDYVAGPNHVLPTGGTARFSSALATEDFVTRL
                     SVIEYSPEGLAEAGPHVGELSRVEGLDGHGAAAAIRIARAGDKR"
     gene            complement(2209..2847)
                     /gene="hisG"
                     /locus_tag="VFX87_02560"
     CDS             complement(2209..2847)
                     /gene="hisG"
                     /locus_tag="VFX87_02560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX4993815.1"
                     /translation="MTTSLTLALPKGRLLDPALALLAGMGIRGIEPDSRRLLLTDEAA
                     GLRFILLKPADIPTYVEYGAADLGIVGKDILLEQQPDVYEPVDLGFGFCRLVVAEPRE
                     LWERDDPSKWSWVRVATKYPRLTEEYFSERGIQVEMVKLDGSIELAPLVGLAERIVDL
                     VQSGETLRANGLVEVAEILTSTARLIVNRASLKTQHARVNQLITAMREKVAR"
     gene            complement(2844..4100)
                     /gene="murA"
                     /locus_tag="VFX87_02565"
     CDS             complement(2844..4100)
                     /gene="murA"
                     /locus_tag="VFX87_02565"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4993816.1"
                     /translation="MAARLIVEGGAPLRGEVAASAAKNAALPALCAALLTARPVVLEN
                     VPELADVTTTRSLLERLGGVVSTEADGGTRVEVAKVTSNEAPYELVSTMRASVLVLGP
                     LLARTGLAKVALPGGCAIGVRPIDQHLKGFQKLGAEVFIRNGYVEARASRLKGARIST
                     DLVTVTGTENLMMAAALAEGTTVIENAAREPEVTDLARLLSAMGARIHGAGTERIEIE
                     GVPELGGARHRVIPDRIEAGTLLVAAAITGGDVTVTGTEPRHMSAMLTKLEESGSAIT
                     VADDRIRCQGPARPKAVDLITNPFPGFPTDMQAQFMAMLGLADGISKITETIFENRFM
                     HAAELARLGARIEAEGSTAVVRGQRYYQGAPVMATDLRASASLVLAGLAARGRTEISR
                     VYHLDRGYERLEVKLRSLGATIERLR"
     gene            complement(4100..5029)
                     /gene="prmC"
                     /locus_tag="VFX87_02570"
     CDS             complement(4100..5029)
                     /gene="prmC"
                     /locus_tag="VFX87_02570"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX4993817.1"
                     /translation="MIGVAADSTVPALLGRAAATLEEAGVATARPEAEWLLAGLLDVE
                     RFALYLEPTRRLTEAEAAHYRELIERRARREPLQYLLGWEDFHGLRLAVTPDVLVPRP
                     ETEGLVEWALEVLAGRPDPVIADLGTGSGAIACALAAARPRAEVLAVEVAAGALAVAS
                     RNVRELGLSARVRLLAGDLFAPLGSLSASLDLVVANPPYLRSAVIASLPPEVSRHEPR
                     AALDGGPDGLGVLRRIVAGAPPVLKPGGWLLMEIGEEQAGPLASLMAAEGFSDIRARR
                     DLNGVERYIGGRWEDTRTTLAVCASVAARRETC"
     gene            complement(5026..6096)
                     /gene="prfA"
                     /locus_tag="VFX87_02575"
     CDS             complement(5026..6096)
                     /gene="prfA"
                     /locus_tag="VFX87_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX4993818.1"
                     /translation="MFDKLRQIEERYGELNRSLSDPQVIGQPAVYAKTAKAAADLAET
                     VRKFDDYKSVLARVSEARHILAEDADREMRELAQAEIDELTARQAKLEEELRSLLIPR
                     DPNDDKNVFLEIRAGAGGDEAGLFAADLFRMYTKYAERQRWKWEVIDSHPTGVGGLKE
                     VVALVQGRGAWSRLKYERGVHRVQRVPATESSGRIHTSTVTVAVLPEAEDVDVKVDEK
                     DIQVDVYRSSGPGGQGVNTTDSAVRLTHLPTGLVVTCQDERSQIKNRAKALRVLKARL
                     LERAQEEQAAAIAADRRSQVGTGERSERIRTYNFPQGRVSDHRINLTLHRLPAVLEGD
                     LDELIDGLRDREQSQKLAESVT"
     gene            complement(6209..6424)
                     /gene="rpmE"
                     /locus_tag="VFX87_02580"
     CDS             complement(6209..6424)
                     /gene="rpmE"
                     /locus_tag="VFX87_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX4993819.1"
                     /translation="MKAGIHPDYVDTTITCNCGEVIHTRSTVPTIKVEVCSKCHPFFT
                     GKQRFLDTAGRVERFQQKYKRKKTTEG"
     gene            complement(6488..7741)
                     /gene="rho"
                     /locus_tag="VFX87_02585"
     CDS             complement(6488..7741)
                     /gene="rho"
                     /locus_tag="VFX87_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX4993820.1"
                     /translation="MNLSELKDKTITDLNNVAKELGVQGTSGLRKQDLIFKILQGQAE
                     RDGLIFAEGVLEVLPDGFGFLRAPDYNYLPGPDDIYVSPSQIRRFDLRTGDTISGQVR
                     PPKDSERYFALLKVEAINFETPEQARDKTLFDNLTPLYPMERIRLEHDSDNLTTRAMD
                     LLCPIGKGQRGLIVAAPRTGKTVLLQNLANAIAKNHPEVYLIVLLIDERPEEVTDMQR
                     SVKGEVISSTFDEPPQRHVQVADMVIEKAKRLVEHKRDVVILLDSITRFARAHNAVIP
                     SSGKVLSGGLDANALQRPRRFFAAARNVEEGGSLTIMATALIDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLMDKRIFPTINIEQTGTRKEELLLEKDELQKVWLLRKALSQLNPVEA
                     MELLLDKLKMMKTNKDFLNQMNQLA"
     gene            complement(7967..9049)
                     /locus_tag="VFX87_02590"
     CDS             complement(7967..9049)
                     /locus_tag="VFX87_02590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4993821.1"
                     /translation="MSPRGVAAIALLVFPLMVSGCSIPTWVPLLGKSKPAAPAEVAVA
                     QPSASAPILTSRDQLHASEDVVDRVICVVNNDAITQYELDEAELYYLAETRERPSDGE
                     ARKVLRTQLLQNLIENRIQLQQAEREKVVIEDAELAESLSDIMRKLKAKDEAEFEGLI
                     KTQGLTLEGVKKRLREQLMVQRVVRRKVALRISVTEQEIDRYLTENREKLETGLTFAA
                     RHILVMPDPTKGEEGWTAARLRADEIYGHLLEGQDFIELAKKYSDDPSGKDGGSLGNL
                     KRGELAQDIEEAILRLSPGEASTPFRSKIGYHLFRLDSREALTGDALVQARGQIREIL
                     YRQKYDTRLKDWLVEIKQRAIIDIRM"
     gene            complement(9046..12300)
                     /gene="mfd"
                     /locus_tag="VFX87_02595"
     CDS             complement(9046..12300)
                     /gene="mfd"
                     /locus_tag="VFX87_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX4993822.1"
                     /translation="MTVPAGSVLSRWAALEELLAALTKGAPCLRAEGLWGSSRALVVA
                     ALRERTGRPILLLTPGPSQRHRMAQDAAFFISSLSGGPATGDDGEHSRVLEFPSGTGT
                     SWRGTRHREPDAERALCCRRLLDGDAVVIVATPSGLSEPVPPPSAFKRRTFSLMVGES
                     SDREILLELLQGAGYERVETVMEVGQWSVRGGIVDIFSPTHERPVRAEFVGDEVESLR
                     VFDPTTQRSIAPLRELTVLPLGSKDAAPVTLVDYLPPETLVVLEDPALLEAPPDDAPS
                     AAPLASVLERFQRLELPLLQRGEGAGPRVSMGTRGVGGYRGQFKTLAGEIRTWRGEGF
                     TVRLVVDDERQSDRLRQMLAEHDLEPWPEGTLWSQEGLAVIVGECAAGFQIPPMGLIV
                     LCEEEIFGAQRRRLRRPLFQRGAAIASFNDLAANDLVVHEQHGIGRYHGLRTLSSEGH
                     DADFLLLEYADGGRLYLPVERLDLISKYMGAPEGSARLDRLGGGAWQRLKESVRAALR
                     DMAEALLKLYATRSVAERPHFSDDTPWQGEFEAAFRFEETPDQLRAIEEVKTDMVGSR
                     PMDRLVAGDVGYGKTEVALRAAFKAVADGRQVAVLVPTTVLAQQHYNTFVERFGPFPA
                     RVELLSRFRSPKEQKQVVAGLAAGTVDVVVGTHRLLSKDVSFKNLGLLVVDEEHRFGV
                     SHKERLKQLRTSVDVLTLTATPIPRTLHMALSGVRDLSVIETPPLDRLPVETVVTPFS
                     RGVIKEAIERELNRGGQVFFVHNRIQSLASMTSFIKSLVPEARVVMGHGQMAERELEG
                     VMVKFVDGQADVLVSTAIVESGLDIPASNTIIINRADRFGLAQLYQLRGRVGRERQQA
                     YAYLLVPADGRVDETAQRRLRVIEESTELGAGLRLAMRDLEIRGTGNLLGAAQHGHIA
                     AVGFDLYSKLLAEAVRDLKGEPAAERVDPVIAADVEALLPEAYVPEVNQRLALYKRLA
                     EIERPEDVSDARAELTDRYGPPPPAAEGLLDVVGLRVAARGLGIERVEAGGGRAVLTF
                     AASTPVTPERMLKAIAASRGALKLRKEYTVEARIPAEPWPAVRDALAKLLDSLR"
     gene            complement(12350..13000)
                     /locus_tag="VFX87_02600"
     CDS             complement(12350..13000)
                     /locus_tag="VFX87_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX4993823.1"
                     /translation="MARRSPFKIPRIPEMTVRRLSVYTRSLLQLEEDGVKTISSQELA
                     ERFSLNSAQVRKDLAYFGEFGVRGIGYYVSGLKAELQRILGLDREWQVALVGFGNLGS
                     ALFHYKGFARQGFRISTIFDEDPAKWGKAVDGVAIQPMEDLPREAKARNLQIAIVAVP
                     GESAQVVTDKLVAAGIKTILNFAPGRIKAGKDVRLKNVDLAIELETLSFYLAQGSR"
     gene            complement(13108..13425)
                     /locus_tag="VFX87_02605"
     CDS             complement(13108..13425)
                     /locus_tag="VFX87_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX4993824.1"
                     /translation="MQRIVTMSMMAILAVPGMAWAADAAGGADMAKAFAVLAAGLGFG
                     LAAGLCGLGQGRAAGQAVDAMARQPGAIARIQTAMLLGLAFIESLVLFVFVIAVILLF
                     VKS"
BASE COUNT         1948 a         4920 c         4671 g         2091 t
ORIGIN      
        1 gcctggaggc tgcccatcgc gtcgtgaaag gccccgtcgc cgggcagcac caccgcctcc
       61 gcgtcgtcca ccaccttcgg gtcctgggtg acgacggcgg gcatcccgat ccgcgcgaag
      121 gccttctcca cgctgccgag attgccgcgc ccgtagtcga cgatggcgat catgtctccg
      181 ggtttgctcc ccgttctacc ggaagcttct ctttcttagt ccgtgcacgt ggaagatatc
      241 gcgctgcggt ttggcctctt cgcgccttcg gcgctcaacc cgcttcacag gctgcccttt
      301 gtggagagga tgcccgtgat tctcgggttg agacgggtgg cctcggcgag ggcgcggccg
      361 gccgccttga aggtggcctc cgcgatgtgg tggaggttct cgccgtagtg catgttgagg
      421 tgcagcgtga tctccgcgtt caccgccacc gctcggaaga agtccttgag cagatcgaga
      481 tcgaagttgg cgatccgggt gcgctggagc ggcacgttga agacgagata ggggcgcccc
      541 gagatgtcca ccgcggcgta cagcagcgcc tcgtccatgg gcaggaacga ggccccgtag
      601 cggacgatcc ccttcttgtc gccgaccgcc tcgcggagcg ccttgcccag gcagatcccc
      661 acgtcctcca ccgtgtggtg gagatccacg tcgaggtcgc ccttggcgtc cacgtggagg
      721 tccatcaagc cgtgcttgga ccacgcctcc agcatgtggt tgaagaacgg gatgccggtg
      781 tccaccttgg cggcgccggt accgtccagg ttgagcgtga gcacgatctg cgtctccttc
      841 gtgttgcgct cgatccggct ctgccgcggc accgccggct ccgtctctcc ccgctccccg
      901 gtcatcgctt gtctcccgcg cgcgcgatcc gtatcgcggc cgccgcgccg tgtccgtcca
      961 gaccttccac gcgggacaac tcgccgacgt gcggccccgc ttcggccaac ccctcgggcg
     1021 aatactcgat cacgctgagc cgcgtcacga agtcctcggt cgcgagcgcc gaggagaagc
     1081 gcgcggtgcc gccggtcggc agcacgtgat tgggccccgc cacgtagtcc cccaccacct
     1141 cgggagtgtg gcgcccgagg aacaccgcac ccgcgtgccg cacccgtacc agcaggccct
     1201 ccggatccgc gacctgcagc tccagatgct ccggcgcgag cccgttggcc aggtcgaccg
     1261 cctcctcgag gctcgcggtg agcacgagcg cgccgtggct cgcgagcgcc gcccccgcga
     1321 tctcccgccg gggcagcccc gcgagctgcc ggccgagctc ggccgccacc gcgtcgcgca
     1381 gcgcgtcgga cggcgtcagc agcaccgcgc gggccatcgg gtcgtgctcg gcctgggcca
     1441 ggagatcggc ggcgacgaag gcggcatcgg cggtgtcgtc ggccaccacc aggacctcgc
     1501 tcgggcccgc caccatgtcg ataccgacgt cgccgaagac ctgagccttc gccagggcca
     1561 cgtagatgtt gccggggccc acgatcttgt ccacgcgtcg gatggtctcg gtgccgtagg
     1621 ccagcgcggc gatcgcctgc gccccgccca cgcggtaggc ctcggtcacg cccgcgatgc
     1681 gcgcggcggc cagtaccgcc gcgttcagcg agcggtcggc cgatggcggc gagaccagca
     1741 cgatctcgcg gaccccggcc acgaccgcgg gcaccaccgt catcagcacg gtggagggat
     1801 aggcggcgcg gccgcccggc acgtagaccg ccacgcgatc gagcggccgc acttcctggc
     1861 cgagccgcga gccgtggtcg tccttcatgc gccaggagcg cggcgcgcac tcgcgatgga
     1921 agcgctcgac gcgctccgcc gcgtagcgca gcgagcgcag cgtcgcctcg cccaccgcgc
     1981 gctcggccgc cgcgtattcc tccggccgca ccgccagctc cggcgcggtc agcgtcaccc
     2041 gatcgaagcg ccgggtgaag tcgagcagcg ccgcgtcgcc cttctcccgc accgccgcga
     2101 cgatctccgc gacccgtcgc gagatctcgg gatccaccga gccgggcggg cgctccagcg
     2161 cgcggatcac acccgccacg ccgaggcctg aggtctcgag cttgcggatc atcgcgcgac
     2221 tttctcgcgc atcgcggtga tgagctggtt cacgcgcgcg tgctgggtct tgagcgaggc
     2281 ccggttgacg atgagccgcg cggtggaggt caggatctcc gccacctcca cgagcccgtt
     2341 ggcccgcaag gtctcaccgg actgcaccag gtccacgatg cgctcggcca ggccgaccag
     2401 cggggccagc tcgatggagc cgtcgagctt caccatctcc acctggatcc cgcgctccga
     2461 gaagtactcc tcggtcagac gcgggtactt ggtggccacc cgcacccagg accacttgga
     2521 cggatcgtcg cgctcccaca gctcgcgggg ttccgccacc accaggcggc agaagccgaa
     2581 gcccaggtcc accggctcgt acacgtccgg ctgctgctcg agcaggatgt ccttgcccac
     2641 gatgccgaga tcggcggcgc cgtactccac gtaggtcggg atgtcggccg gcttgagcag
     2701 gatgaagcgc agccccgccg cctcgtcggt cagcagaagc cgccgcgaat cgggctcgat
     2761 gccgcggatg cccatgcccg cgagcagggc cagcgcggga tccagcagcc ggcccttggg
     2821 cagcgcgagc gtgagcgacg tcgtcaccgc agtcgctcga tggtggcgcc gagcgagcgc
     2881 agcttgacct cgagccgctc gtagccccgg tccaggtgat agacgcgcga gatctcggtg
     2941 cgccctcggg cggccagccc ggccaggacc agcgaggcgg aggcgcgcag gtcggtggcc
     3001 atgaccggcg cgccctgata gtagcgctgg ccgcgcacca ccgcggtgga gccctcggcc
     3061 tcgatcctcg cgcccagccg cgccagctcg gccgcgtgca tgaagcggtt ctcgaagatg
     3121 gtctcggtga tcttgctgat cccgtcggcc agcccgagca tcgccatgaa ctgggcctgc
     3181 atgtcggtgg ggaagcccgg gaacggattc gtgatcaggt ccaccgcctt cggccgggcg
     3241 gggccctggc agcggatgcg atcgtccgcg accgtgatcg cggacccgga ctcctccagc
     3301 ttggtgagca tcgcggacat gtggcgcggc tcggtgccgg tcaccgtcac gtcgccgccg
     3361 gtgatggcgg cggccaccag cagggtgccc gcctcgatgc ggtccgggat cacgcggtgc
     3421 cgcgcgccgc ccagctccgg cacgccctcg atctcgatgc gctcggtgcc cgctccgtgg
     3481 atccgggccc ccatggcgga gagcagccgc gccaggtcgg tcacctccgg ctcgcgcgcc
     3541 gcgttctcga tcacggtggt accctcggcg agcgcggccg ccatcatgag gttctcggtg
     3601 ccggtcaccg tgaccaggtc ggtgctgatg cgggcgccct tgagccggct ggcccgcgcc
     3661 tccacatagc cgttgcggat gaagacctcc gcgcccagct tctggaagcc tttcagatgc
     3721 tggtcgatgg gccgcacccc gatggcgcag ccgccgggca gggccacctt ggcgaggccg
     3781 gtccgcgcga gcagcgggcc cagcaccagg acggaggcgc gcatggtcga gaccagctcg
     3841 tacggcgcct cgttgctcgt caccttcgcc acctcgacgc gcgtgccgcc gtcggcctcg
     3901 gtcgagacga ccccgccgag ccgctccagc agcgagcggg tggtggtcac gtcggccagc
     3961 tcgggcacgt tctccagcac caccggccgc gcggtgagca gcgccgcgca cagcgcgggc
     4021 agcgccgcgt tcttggccgc gctcgccgcg acttcccccc gcagcggcgc tccgccttcg
     4081 acgatgagtc gggccgccat cagcaggtct cccgtcgcgc cgcgacggaa gcgcagaccg
     4141 cgagtgtggt ccgcgtgtct tcccagcgcc cgccgatgta ccgctccacg ccgttgagat
     4201 cccgccgggc ccggatgtcg gagaagccct cggcggccat gagcgaggcg agcgggcccg
     4261 cctgttcctc gccgatctcc atcagcagcc acccgcccgg cttgagcacc gggggcgccc
     4321 ccgccacgat gcggcggagc acgccgaggc cgtccgggcc gccgtccagc gccgcgcgcg
     4381 gctcgtggcg cgacacctcg ggcggcagcg acgcgatgac cgcgctccgc aggtacggcg
     4441 ggttggccac caccaggtcg aggctcgccg acagcgagcc gagcggcgcg aagaggtcgc
     4501 ccgcgagcag ccgcacccgc gcggagagcc cgagctcgcg gacgttgcgg gacgcgacgg
     4561 cgagggcgcc cgccgccacc tcgacggcca gcacctccgc gcggggccgc gccgccgcga
     4621 gcgcgcaggc gatggcgccg ctgccggtgc cgaggtcggc gatcaccggg tcgggacggc
     4681 ccgcgagcac ttccagcgcc cactccacga ggccttcggt ctccggacgc ggcaccagca
     4741 cgtcgggggt gacggccagg cgcaggccgt ggaaatcctc ccagccgagc aggtactgga
     4801 gcggctcgcg gcgcgcgcgg cgctcgatca gctcccggta gtgggcggcc tccgcctcgg
     4861 tcagccggcg cgtcggctcc aggtagaggg cgaagcgctc gacgtccagg agcccggcga
     4921 ggagccactc ggcctccggg cgcgccgtcg cgaccccggc ctcctcgagc gtggcggccg
     4981 cgcgcccgag cagcgcgggg acggtcgaat ccgctgcgac cccgatcacg tcacgctctc
     5041 cgcgagcttc tggctctgct cgcgatcgcg caggccgtcg atcagctcgt ccagatcgcc
     5101 ctcgagcacc gcgggaagcc ggtgcagcgt gagattgatg cggtgatcgc tgacacgccc
     5161 ctggggaaag ttgtaggtcc ggatgcgctc gctgcgctcc ccggtgccga cctggctgcg
     5221 ccggtcggcc gcgatggcgg cggcctgctc ctcctgggcc cgctccagca ggcgggcctt
     5281 cagcacccgc agggccttcg cgcggttctt gatctgcgag cgctcgtcct ggcaggtgac
     5341 gaccagcccg gtcggcaggt gggtgagccg caccgcggag tcggtggtgt tgacgccctg
     5401 cccgcccggc cccgaggagc ggtagacgtc cacctggatg tccttctcgt ccaccttcac
     5461 gtccacgtcc tccgcctccg gcagcaccgc caccgtcacg gtcgaggtgt ggatgcgccc
     5521 gctcgactcg gtggcgggca cgcgctgcac gcggtgcacg ccccgctcgt acttgagccg
     5581 actccacgcc ccgcggccct gcaccagcgc gacgacttcc ttgaggccgc ccaccccggt
     5641 ggggtggctg tcgatgacct cccacttcca gcgctgccgc tccgcgtact tggtgtacat
     5701 gcggaagagg tcggccgcga agaggccggc ctcgtcgccg cccgcgccgg cccggatctc
     5761 caggaagacg ttcttgtcgt cgttgggatc gcgcgggatg aggaggctcc gcagctcctc
     5821 ctcgagcttg gcctgccgcg cggtcagctc gtcgatctcg gcctgggcca gctcgcgcat
     5881 ctcgcggtcg gcgtcctccg ccaggatgtg gcgggcctcc gagacgcggg cgaggacgct
     5941 cttgtagtcg tcgaacttcc gcaccgtctc ggccaggtcg gccgcggcct tggcggtctt
     6001 cgcatagacc gcgggctgcc cgatcacctg gggatcggac agcgagcggt tcagctcgcc
     6061 gtaacgctct tcgatctgcc ggagcttatc gaacatgggg gcgcctcagg gagtgaaacc
     6121 cggaagagga cgccgccgcg gctggaaacg accgagtaac ggatggtgga tctgcggctc
     6181 ggagcgcggc gcggcgccga gccgggccct acccctcggt ggtcttcttc ctcttgtact
     6241 tctgctggaa gcgctcgacc cgcccggcgg tgtccaggaa ccgctgcttc cccgtgaaga
     6301 aggggtggca cttggagcag acctccacct tgatggtcgg cacggtagag cgggtgtgga
     6361 tgacttcccc gcagttgcag gtgatcgtcg tatcaacgta gtccggatga atgccagcct
     6421 tcacggcgca cctcctcgat ggacctcaga gaatagcagg gagagcgtca aaaggcaaaa
     6481 ctccggccta cgccagctgg ttcatctggt tcaggaagtc cttgttagtc ttcatcattt
     6541 tgagcttgtc gagcagcagc tccatggcct cgacggggtt gagctgggac agggccttgc
     6601 gcaggagcca caccttctgc agctcgtcct tctccagcag gagctcttcc ttccgggtgc
     6661 cggtctgctc gatgttgatg gtcgggaaga tccgcttgtc catcaatcgc cggtccaggt
     6721 gcacctccat gttgccggtg cccttgaact cctcgaagat cacgtcgtcc atccgggagc
     6781 cggtgtcgat gagggcggtg gccatgatgg tgagcgagcc gccctcctcc acgttccggg
     6841 ccgccgcgaa gaaccggcgg ggccgctgga gcgcgttggc gtcgagaccg cccgagagca
     6901 ccttgccgga ggacgggatg accgcgttgt gggcgcgcgc gaaacgggtg atcgagtcga
     6961 gcaggatcac gacgtcgcgc ttgtgctcga ccagccgctt ggccttctcg atcaccatgt
     7021 ccgccacctg cacgtgccgc tgcggcggct cgtcgaaggt ggaggagatg acctcgccct
     7081 tcaccgagcg ctgcatgtcg gtgacctctt ccggtcgctc gtcgatgagc agcacgatca
     7141 ggtacacctc ggggtggttc ttggcgatgg cgttggcgag gttctgcagc agcaccgtct
     7201 tgccggtgcg cggggccgcc acgatcaggc cgcgctgacc cttgccgatg gggcagagca
     7261 ggtccatggc ccgcgtggtc aggttgtccg agtcgtgctc gagccggatc cgctccatcg
     7321 ggtagagcgg cgtcaggttg tcgaagagcg tcttgtcgcg cgcctgctcc ggcgtctcga
     7381 agttgatcgc ctcgaccttg agcagcgcga agtagcgctc gctgtccttc gggggccgca
     7441 cctgcccgga gatggtgtcc ccggtgcgca ggtcgaagcg ccggatctgc gagggcgaga
     7501 cgtagatgtc gtcggggccc ggcagatagt tgtagtccgg cgcgcggagg aagccgaagc
     7561 cgtcgggcag cacttccagc acgccctccg cgaagatgag cccgtcgcgc tcggcctgcc
     7621 cctgcaggat cttgaagatc agatcctgct tgcgaaggcc gctggtgccc tggacgccca
     7681 gctccttggc gacgttgttg agatcggtga tggtcttgtc cttgagctcg gacaggttca
     7741 tccgctttcc tcctgcaccg accgccgcgc cggtctccgt aggagtgggc tgctcggcgg
     7801 ggacggcaga cggcggcttc ggcgagggac gcttcgggct acgctctgaa gaagagatag
     7861 tcgtctccgg cgggttgcgt gtgtgcgggt ctactctcta ggaggttggc tgccgtgctt
     7921 cgcgaaccac tgtcccacac ggtttcccgc cctgtcaacc cccgatttac atgcggatat
     7981 cgatgatcgc gcgctgcttg atctccacga gccagtcctt gagccgcgtg tcgtacttct
     8041 ggcggtagag gatctcgcgg atctggccgc gggcctgcac gagcgcgtcc ccggtgaggg
     8101 cctcgcgcga gtcgagacgg aagaggtgat agccgatctt cgagcggaac ggcgtcgagg
     8161 cctcgcccgg ggacagacgc aggatcgcct cctcgatgtc ctgggccagc tcgccgcgct
     8221 tgagattgcc gagggagccg ccgtccttgc cggacggatc gtccgagtac ttcttggcca
     8281 gctcgatgaa gtcctgcccc tcgagcagat gcccgtagat ctcgtcggcc cggagccgcg
     8341 ccgccgtcca gccctcctcg cccttggtcg gatccggcat caccaggatg tgccgggccg
     8401 cgaaggtgag cccggtctcg agcttctcgc ggttctccgt gaggtaccgg tcgatctcct
     8461 gctcggtgac cgagatgcgc agggcgacct tgcggcggac cacccgctgg accatcagct
     8521 gctcgcgcag ccgcttcttg acgccctcca gggtgaggcc ctgcgtcttg atcagcccct
     8581 cgaactcggc ctcgtccttg gccttgagct tcctcatgat gtcgctgagc gactcggcaa
     8641 gctccgcgtc ctcgatcacc accttctccc gctcggcctg ctggagctgg atccggttct
     8701 cgatcaggtt ctgcagcagc tgcgtgcgca gcaccttgcg cgcctcgccg tcggagggcc
     8761 gctcccgcgt ctcggccagg tagtagagct ccgcctcgtc cagctcgtac tgggtgatcg
     8821 cgtcgttgtt caccacgcag atcacgcggt ccaccacgtc ctcggaggcg tggagctgat
     8881 cccgcgaggt caggatgggc gcggaggcgg acggctgcgc gacggccacc tccgccggcg
     8941 cggccggctt gctcttgccg agcagcggca cccaggtcgg gatcgagcaa cccgagacca
     9001 tcaaggggaa aaccagcaac gcgatcgcgg cgaccccgcg cgggctcatc ggagggaatc
     9061 caggagcttc gcgagggcgt cgcgcaccgc aggccagggc tcggcaggga tccgcgcttc
     9121 caccgtgtac tcctttctca gcttgagagc gccgcggctg gccgcgatcg ccttgagcat
     9181 ccgttcgggc gtgaccgggg tcgacgccgc gaaggtcagc accgcgcgcc cgccgcccgc
     9241 ctccacccgc tcgatgccga gcccgcgggc ggccacgcgc agccccacca cgtccagcaa
     9301 cccctccgcc gcgggcggcg gcgggccgta gcgatcggtc agctccgcgc gcgcgtcgga
     9361 cacgtcctcg ggccgctcga tctcggccag ccgcttgtag agcgccagcc gctgattcac
     9421 ctcggggacg tacgcttcgg gcaggagcgc ttcgacgtcc gccgcgatca ccgggtcgac
     9481 gcgctcggcc gccggttcgc ccttcaagtc tctcacggcc tccgcgagga gcttgctata
     9541 tagatcgaat ccgacggcgg cgatgtgccc gtgctgggcc gcccccagca ggttgccggt
     9601 gccccggatc tccagatccc gcatggccag tcgtagcccc gccccgagct cggtggactc
     9661 ctcgatcacc cgcagccgcc gctgggccgt ctcgtccacc cgcccgtcgg cgggcaccag
     9721 gaggtaggcg taggcctgct gccgctcccg ccccacccgc ccccggagct ggtagagctg
     9781 ggccaggccg aaccggtcgg cccggttgat gattatcgtg ttcgaggccg ggatgtccag
     9841 gcctgattcc acgatggccg tggagaccag gacgtcggcc tggccgtcca cgaacttgac
     9901 catcacgccc tccagctccc gttccgccat ctggccgtgc cccatgacca cccgggcctc
     9961 cgggaccagg ctcttgatga agctggtcat ggaggcgagc gactggatgc ggttgtggac
    10021 gaagaagacc tgcccacccc ggttcagctc ccgctcgatc gcctccttga tgacgccgcg
    10081 actgaacggg gtcaccaccg tctccaccgg cagccggtcg agcggcggcg tctcgatgac
    10141 cgagaggtcg cgcaccccgg acagcgccat gtgcagcgtg cgcgggatcg gggtggcggt
    10201 gagcgtcaac acgtccaccg aggtgcggag ctgcttgagc cgctccttgt gggagacgcc
    10261 gaagcggtgc tcctcgtcca ccaccaggag cccgagattc ttgaacgaca cgtccttgga
    10321 caggagccgg tgcgtgccca ccaccacgtc caccgtgccc gcggcgagtc ccgccaccac
    10381 ctgcttctgc tccttcgggc tgcggaagcg cgagagcagc tcgacccgcg ccgggaacgg
    10441 gccgaagcgc tccacgaacg tgttgtagtg ctgctgggcc agcacggtgg tgggcacgag
    10501 caccgccacc tggcgaccgt ccgccaccgc cttgaacgcc gcgcggagcg cgacctccgt
    10561 cttgccgtag ccgacatcgc ccgccaccag acggtccatc ggccggctac ccaccatgtc
    10621 ggtcttcact tcctcgatgg cgcggagctg gtccggggtc tcctcgaagc ggaaggcggc
    10681 ctcgaactcg ccctgccagg gcgtgtcgtc cgagaagtgc ggccgctcgg cgaccgagcg
    10741 cgtggcatag agcttgagca gggcctccgc catgtcgcgc agcgcggccc gcaccgattc
    10801 cttcaggcgc tgccacgccc cgcccccgag ccggtcgagc cgcgccgagc cctcgggggc
    10861 gcccatgtac ttggagatca ggtcgagccg ctcgacgggc aggtagagcc ggccgccgtc
    10921 ggcgtactcc agcagcagga agtcggcgtc gtgcccctcg gaagagagcg tgcggaggcc
    10981 gtggtagcgg ccgatgccgt gctgctcgtg caccaccagg tcgttggcgg ccaggtcgtt
    11041 gaacgaggcg atggccgcgc cgcgctggaa gagcgggcgc cggaggcggc ggcgctgggc
    11101 cccgaagatc tcctcctcgc acagcacgat gagacccatc ggcgggatct ggaagcccgc
    11161 cgcgcactcc cctacgatca ccgcgagccc ttcctggctc cagagcgtcc cctccggcca
    11221 cggctcgaga tcgtgctcgg ccagcatctg gcgcagccgg tcgctctgcc gctcgtcgtc
    11281 caccaccagg cgcaccgtga agccctcgcc ccgccaggtg cggatctcgc cggccagcgt
    11341 cttgaactga ccgcggtagc cgcccacgcc acgcgtgccc atcgacacgc gcggccccgc
    11401 gccctcgccg cgctggagca gcggcagctc gaggcgctgg aaccgctcga gcacggaggc
    11461 gagcggcgcc gccgagggcg catcgtccgg cggcgcctcc agcagggcgg gatcctccag
    11521 cacgaccagc gtctcggggg gcaggtagtc cacgagcgtc accggcgccg catccttcga
    11581 gcccagcggc agcacggtca gctcgcgcag cggcgcgatc gagcgctgcg tggtgggatc
    11641 gaagacgcgc agggactcga cctcgtcgcc gacgaactcg gcgcgcaccg gccgctcgtg
    11701 ggtgggcgag aagatgtcca cgatgccgcc gcgcacgctc cactgcccga cctccatcac
    11761 ggtctcgacg cgctcgtagc cggcgccctg gagcagctcg agcaggatct cgcggtcgct
    11821 gctctccccg accatcaagc tgaacgtgcg acgcttgaag gcggagggcg gcggcaccgg
    11881 ctccgagagc ccggagggcg tggccacgat cacgaccgcg tcgccgtcga gcaggcgccg
    11941 gcagcacagc gcgcgctcgg cgtcgggctc gcggtggcgg gtgccacgcc aggaggtgcc
    12001 ggtgcccgag gggaactcga gcacgcggct gtgctcgccg tcgtcgccgg tcgcggggcc
    12061 gcccgagagc gaggagatga agaaggcggc gtcctgcgcc atccggtgac gctgcgacgg
    12121 gcccggcgtg agcaggagga tcggccggcc ggtgcgctcc cgcagcgccg ccactaccag
    12181 ggcgcgcgag gagccccaga gaccctcggc gcgcaggcag ggtgctccct ttgtgagggc
    12241 cgcgaggagc tcttcgagcg cggcccagcg ggacaggacg gacccggcag ggacggtcat
    12301 cacggacgac ggcacatcag aggtaccacc ggcaccgacc cccgtcaaat cagcgggagc
    12361 cctgggcgag gtagaaggag agcgtctcca gctcgatggc caggtccacg ttcttcaggc
    12421 ggacgtcctt gcccgccttg atgcgccccg gcgcgaagtt gaggatggtc ttgatccccg
    12481 cggccaccag cttgtcggtg acgacctggg cggactcccc gggcaccgcc acgatcgcga
    12541 tctggaggtt acgcgccttg gcctcgcgcg gcaggtcctc catgggctgg atggcgactc
    12601 cgtccaccgc cttgccccac ttggccggat cctcgtcgaa gatcgtcgag atccgaaagc
    12661 cctggcgggc gaagcccttg tagtgaaaga gggccgagcc gaggttgccg aagcccacca
    12721 gcgccacctg ccactcgcgg tcgagaccca ggatgcgctg cagctcggcc ttgagcccgg
    12781 acacgtagta gccgatgccg cgcaccccga actcgccgaa gtaggcgagg tccttccgca
    12841 cctgggccga gttgaggctg aagcgctccg ccagctcctg ggaggagatg gtcttgacgc
    12901 catcctcttc taactggaga agacttcgcg tgtagacgga caggcggcgc accgtcatct
    12961 ccgggatccg gggaatcttg aacggtgacc gtcgcgccat gttcgttccc gggcgctcgt
    13021 tgcgcccgtc agggccgcct cgcgggaatg cccgcgtgcc gtcccggggc gtggccccgg
    13081 gacggtgtct tcgagtgtgc cgctacctta cgacttgacg aacagcagga tgaccgcgat
    13141 gacgaacacg aacagcacga gcgactcgat gaaggccaga ccgagcagca tcgcagtctg
    13201 gatccgggcg atcgctccgg gctgccgcgc catcgcgtcc accgcctgcc cggccgcacg
    13261 gccctgaccg agaccgcaga gacccgcggc cagtccgaag cccaggcccg cggccagtac
    13321 cgcgaaggcc ttcgccatgt cggccccacc cgcggcatcg gctgcccagg ccatccccgg
    13381 gactgccaga atcgccatca tgctcatggt gacaatgcgt tgcacggctt tcctccttgt
    13441 tgagggtgac tgcgacctag tgatgctcgg cttcctcgac cgccgccgag atgtacagca
    13501 tggccagctt gcagaagatg aaggtctgga tgaaagcgac caggatcttc agggggaaca
    13561 agaagcccac gacgaacacg accatgatca gcgcggcggg cgccccgatc accagtcccg
    13621 ccaccgagcc
//
LOCUS       DASUFA010000069        18153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1269275, whole genome shotgun sequence.
ACCESSION   DASUFA010000069 DASUFA010000000
VERSION     DASUFA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 18153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18153
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269275"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFX87_02610"
     CDS             complement(<1..188)
                     /locus_tag="VFX87_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX4993825.1"
                     /translation="MRQLSGLDTMFLNLETNAVPMHVGGLTVIDPASAPKAFGFAAVR
                     RLIEGRLHLLPMFRRRLV"
     gene            complement(236..1795)
                     /locus_tag="VFX87_02615"
     CDS             complement(236..1795)
                     /locus_tag="VFX87_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4993826.1"
                     /translation="MIGAGHNGLVTACYLARAGLRVLVLERRYLVGGACVTEETFPGF
                     KVSTAAYVNSLFRKEIVRDLRLGDYGFEVLPRSPSSFTPLPDGRSLTLGPDAELTRTE
                     IARFSARDAARYPEYEAMLERVAAFIEPALGMTPPDVLHPGPRGLLQLLGLGRRFARL
                     GDDASEAVELLTGPARTILDRWFESTPLKATLATDAIIGAMASPSTPGTGYVLFHHVM
                     GETDGRRGVWGYVRGGMGGLTQALARAARDLGVEIRCEAPVARIAVRDGRVTGVVLES
                     GEECRARIVASNADAHVTFLRLLAPETLPPGFRDAVARISYESASLKINLALGELPSF
                     TARPGTAAGPQHRGTIHICPDLDYIERAYDDAKYGRPSERPVLECTIPSVVDPTVAPP
                     GRHLMSIFVQYAPYALRGTSWDQIGEAFADRCVALLDEYAPNFSRSVLARQVLTPLDI
                     ERVFGLTGGNIFQGAMPLHQLFAFRPVPGWSDYRTPLPGLYLCGAAAHPGGGVLGAAG
                     RNAAREMLRDL"
     gene            complement(1828..2805)
                     /locus_tag="VFX87_02620"
     CDS             complement(1828..2805)
                     /locus_tag="VFX87_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993827.1"
                     /translation="MNRRAFIGAVASGCLLASTRRGAAQGTGRVYRVGLLSPGSPSTG
                     IGAFGLGIRRLGWVPGQNLIIEQRYARGDLARLPALAAELVGLGVDVILASSIAIPAA
                     RAATRTIPTVMTFAVDDPVEEGWVASLAQPGGNLTGVTLHAPELTGKRLELVKVVLPG
                     MRRVGVLAWPRPGGLGQVRAAETAARSLGLQPHVVEVQEPSQYDGALDALKREGADAL
                     LVLSSSAFFAERQRIADSAIKHRLPLVAPFREVAEAGGLMAYGPNIVELWGQRVPVYV
                     DRILKGARPGDLPIEQPTKYELVINLKTAKTLGLTIPQSLLLRADQVLE"
     gene            2844..3314
                     /locus_tag="VFX87_02625"
     CDS             2844..3314
                     /locus_tag="VFX87_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEX4993828.1"
                     /translation="MTFEPVTPRLERLCRATVEIAAPLVVGDTPLGLRRVIPITGGRV
                     EGPRLSGEVLPGGADWQIVRADGGAVLEARYTVRTTDGALVYVRNIGLRCGPPEVLAR
                     LGRGEHVDPASYYFRTTPRFETGAPAHAWLNDVIAVGSAVRRADAVVLDFFVVA"
     gene            complement(3342..4337)
                     /locus_tag="VFX87_02630"
     CDS             complement(3342..4337)
                     /locus_tag="VFX87_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993829.1"
                     /translation="MSPGAVANRRAFVTALAAGALLLPGAAPAQPGRVARVGYLTPSS
                     VSARGGIPELRQRLHDLGYVEGRDVRFEIRAANEDFERLPDLAADLVRSGVDLIVAVS
                     SPAIRAAENATSTIPIVMVSGTDPVAGLFVASLARPGSNVTGVTTYLPELAGKRLELL
                     RECLPGLRRVAVLANLRNPSSAAAARETEAAARAQALEIQVVDARLPEQYPDAFASMM
                     RGGARAVFVTTDPILSSNRERIMQLAARHRLPAMYEWPEIVEAGGLLSYGPSLAEVNT
                     RAAAYVDKILKGAKPADLPVERPSRLELAVNLKTAAALGITIPTAVVSRAGRVIR"
     gene            4418..4795
                     /locus_tag="VFX87_02635"
     CDS             4418..4795
                     /locus_tag="VFX87_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993830.1"
                     /translation="MGRARILVVDDERAVRDLISDALKIEGHDVLTASDGKEGLDLIS
                     QHRFDLVFCDLRMPEMDGQALYEEIQRNHPQILKRIVFVTAQANSADYGPFLRTTGIP
                     VIEKPFTLSQLRQAVTRIVGPMR"
     gene            complement(4821..5723)
                     /gene="rho"
                     /locus_tag="VFX87_02640"
     CDS             complement(4821..5723)
                     /gene="rho"
                     /locus_tag="VFX87_02640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX4993831.1"
                     /translation="MPPRHAPRVPSPSIFEGLTAVQPYEKLKLETTRDELSMRALDMI
                     APIGRGQRGLIVAPPRTGKTILLQKIAKAVLANHPEVVVIVLLVDERPEEVTDFKMQI
                     GKQAEIVASSNDNPYRRHIEVTEQVLDKAKRMVEEKRDVLILFDSLTRMTRAYNNELS
                     SRGRTMSGGIDSRAFQMPRAFFGAARKIEEGGSLTIIGTVLVDTGSRMDQIIFEEFKG
                     TGNMELYLSRELADRRIFPSFDLMRSGTRREELLFSEEDMKKIHMLRRALSGTKPQEA
                     MEALLGRLKLTATNADFLKSLGQD"
     gene            complement(6096..7166)
                     /locus_tag="VFX87_02645"
     CDS             complement(6096..7166)
                     /locus_tag="VFX87_02645"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4993832.1"
                     /translation="MTRTSMDKPIAGPMVWHGRELAASTDWIRRLSAGAVDEIDAALR
                     AVQAGGLVWRDITKKDFPLPGVSAELAQVNRDLEWGRGLVLLRGLPVARYSDDALRAI
                     WWGLGCHLGTAVHQNAHGELIGEVRDEVRLYGEVVQPAVHKSEGVTSRAKARSSGPLR
                     WHTDRCDVVGLLCVRSARRGGTSRVVSVPAVSNAILERRPDLHALLCSDYYRTRQGEE
                     AGGEASIYAMPVFAMREGKFTTQYSRTFVEAAQKIATIPRLTPAQDEALDLLAAVCDE
                     LGYEMELEPGDIQLLNNHVIYHGRTAYEDAPEPGRDRLLMRLWLAPPNSRPLPEGFET
                     LWGSIKGGAVRGGIAQSPAAAR"
     gene            7232..8137
                     /locus_tag="VFX87_02650"
     CDS             7232..8137
                     /locus_tag="VFX87_02650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4993833.1"
                     /translation="MAGPRGRLVVGLLGGLLAALLAACAGPASDRSMITTAPNGAREE
                     IPVAVSRPEGPGPFPAVVIMHDCSGLGPRSSGAPGRWARELGDRGYVTVIPDSFTTRG
                     HPDGVCTDASPSRGEVAPSRRVRDAYAALAHARTLPYVDGRRVGLMGGSHGGSTTLAS
                     LAAPQNAWELLAREKRAGFAAAVALYPSCGTTLGAWRADGTGTYRPVAPLLILIGEKD
                     DWTPAASCVKLVASTRATEHPAAITVYPGAHHSFDSDRPVRFVAARVNSNAPGGRGAT
                     TGGDAAAWADSIRRVVEFFGERLRP"
     gene            8151..10514
                     /locus_tag="VFX87_02655"
     CDS             8151..10514
                     /locus_tag="VFX87_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX4993834.1"
                     /translation="MTGSGSSRWAWLAALTAAYVVAGKLGLLFASVHASATAVWPPSG
                     IALAALLILGVRMWPAVAAGAFLVNVTTAGSVATSLGITLGNTLEAVIGCWLVGRLAH
                     GRGALERPRHIFAFSAAVVPGAALAATVGVASLVLGGFAPRADAAAIWITWALGDITG
                     ALIVAPLLILWTADHRSLALRRQPIEALALLLGVVGVALIVFGGLAPAEWRDYPLTFL
                     AIPPLLWAAIRFGRREAATAVVVLMTIAVGGTMRGFGAFAVLPPTHSLLVLQSFVATM
                     ALMTLVVAALVRSREREGSLLQAIIDRIPVMITMYEPNTRALRLNREFERLTGWSSDA
                     ARDVDLMARCYPDPAYCAEIRAYMDSMREGWRDVAMTTRDGRVLQTSWSNIRLPDDTR
                     IGIGLDATERRRSEDERERARAEAEEASRIKDEFFAMLGHELRNPLGAITTALHVIDT
                     GEPGDERSAQAREIVTRQVRHLVRLVDDLLDVTRLATGKITLGRRPVHLAALARRVVG
                     ALAATTPTLNLRSEAGAAVWIEADETRLEQILTNLLGNAVKFTPAGGRVTVAVAARGG
                     EAVLSVEDTGAGIPADLLPRIFDLFVQGQTGLHRSVPGLGIGLTLVKRLVDLHGGRIE
                     AASEGPGRGSVFTVRFPLRPAPHDAADAAPVPGGARVRRRVLIVEDSDDARDMLRHLL
                     EQAGHEVYEAADGLTGLDRALALEPDAAVIDIGLPGLDGYEVARRIRAAGRADALLVA
                     VTGYGQAGDRQRSAEAGFDAHLTKPVDPLALETLLVNLPLDPRRTGR"
     gene            10527..12110
                     /locus_tag="VFX87_02660"
     CDS             10527..12110
                     /locus_tag="VFX87_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HEX4993835.1"
                     /translation="MLSTSPRVTIPDVTITDYVLRHAARLGDKPALVDGPTGRTLTYR
                     QLADGVRRAAAGLAKRGFGKGDVLAIYCPNLPEYAIVFLGVAMAGGVNTTVNPLYTAD
                     ELANQLRDSGARFLVTVPPFLEKAKEGAAKSSVEEIFVLGAADGVRPFTDLMQAGDQP
                     PAVTMNPAEDLVALPYSSGTTGLPKGVMLTHRNLVANLCQAEGMRNFEGFGERDITMA
                     VLPFFHIYGMVVILMLGLAGGGTILVMPRFDMMEFLTFVQKYRVTILPLVPPIVLGLV
                     KHPAVAQFDLSSVRLIFSGAAPLGEEMARALGAKLGCPVVQGYGMTEASPVTHLSPTR
                     NEPFKPGSAGKIVPNTEVKIVEVGSGSDAELPVGKEGELWIRGPQIMKGYLNRPEETA
                     GCLDRDGWYHTGDVGYVDDDGFFFIVDRTKELIKYKGMQVAPAELEALLLTHPAILDA
                     AVVRRADEEAGEVPKAYVVLKPDDVSKATPADAIMGWVAQRVAPHKRIRDLEFIDQIP
                     KSASGKILRRVLIDREKSR"
     gene            12201..13166
                     /locus_tag="VFX87_02665"
     CDS             12201..13166
                     /locus_tag="VFX87_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX4993836.1"
                     /translation="MNKIERVRAALAGRPVDRAPFTVWYHFGNQHAPAERTAQVHLEF
                     FDHYDLDLLKLMNDYDYPMPAGLEVIATAADLGKLQPFDPARTPFGTQLQTVELVARA
                     LRDRALFVDTVFNAWNTLKRNLVREAMPALMVEQPKALEAALAVVNDNLIRYAQASLA
                     RGAAGIFLSVPATTESLTLDQYERFMRPFDLAFLKAIEGRGECHVLHAHGEHLYFDRL
                     VDYPAHALSWADLNGGPSIAEARRRTRFTLMAGLDHVKFVESSARLVRGQVTRALEAG
                     GATGFILAPGCSLPTYAYPPLIRAARDESSKQGGSRLGESQGHKG"
     gene            13271..13489
                     /locus_tag="VFX87_02670"
     CDS             13271..13489
                     /locus_tag="VFX87_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993837.1"
                     /translation="MTPKLMALAAVVVLTVVFLSPVAYMAGQRATVWLTAPAGEPTGP
                     WPSVDEAEREPGPTERSRPTVRPGFGDI"
     gene            complement(13514..14254)
                     /locus_tag="VFX87_02675"
     CDS             complement(13514..14254)
                     /locus_tag="VFX87_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4993838.1"
                     /translation="MSVTVRFVGSGDSFGSGGRFQTCILVDGPRCRFAVDFGASSLIA
                     LAQQGIEHNSIDAILLTHLHGDHAAGVPFLLMDAMLGARRARPLTIAGPREVRARMDV
                     IREALFPGSHVMTPRFPVHWIELEPGRSQPVLDLLVTPSLARHTQETNPTALRIQVGD
                     KTMCYTGDTEWTDDVARAAHDSDLLIAESYFYDKPVKWHLNYPDIVAHAGRVGARRLI
                     LTHMSSEMLARADSVPEECAHDGLVVTL"
     gene            14344..15168
                     /locus_tag="VFX87_02680"
     CDS             14344..15168
                     /locus_tag="VFX87_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX4993839.1"
                     /translation="MRLFDRKFGTGFLEEVPAAPGVYRFHDATGVLFYVGKAANLRRR
                     LGQYRLAGRRKKERKRRALVKAAARITWEVCESPLAAALAEIRLIQTLRPPQNVASAF
                     PFLYPYVGVTTEGAETYFCLTTAPEAFPAFTFHGAFRSREVTGEAFFRLMELLRYVGH
                     PVPRHRCRRLGAARHSYVRGFRRLPADSAETWSALLRGTSREALEALALRLVDHAGAR
                     ARREQTHRALRAVARFFRHEARVLARVRTATGYPLYPVPQRERDLLFARYRVEALP"
     gene            15282..15683
                     /locus_tag="VFX87_02685"
     CDS             15282..15683
                     /locus_tag="VFX87_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993840.1"
                     /translation="MPAEPSSRRILVVDDDDQIRDLLATALSADGHTVDGARDGGEAL
                     ALLAHHPYDLVLSDLHVPRMDGPSLYETLQTRHRFPVRFATKLPRVIFMTGNPAEHTE
                     FLRGTTDPILEKPFAMRIVRKMVRVLLSAAP"
     gene            15694..17157
                     /locus_tag="VFX87_02690"
     CDS             15694..17157
                     /locus_tag="VFX87_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4993841.1"
                     /translation="MRLATKIFLVSALVILVLCGTVVWSLLTVKRLVSANQEIATRAV
                     PALRLQGALREAVQGLVRLEARALVLQDQDYAAMWSDRATRVTRDLEKLGSYVERAEE
                     RTAHRRAREAFATYCGHVKEERRLVALGQTRAALQIAEGPAREAAERAEAALIDATAA
                     TEAGLAAGQARARALEARTWRAVTAALVASLLLALSASAVLAYRMARSLGRLSAATTS
                     LAAGSWTGPLVVTGRDEISELGRAFNQMAERLSEVERLKEEFFSHISHDLRNPLAAIR
                     LSAEALQERAQAAGDPKQARFAYLIDTSAARMMVMINQILDFTRLRARTVPLDRKPTD
                     LLHVITRALDELRPMSEEKRIRVDLAAEGHTFTVLGDEGSLVRVVVNLLGNALHFTPS
                     GGAVRARLAELPDRLELDLEDTGVGIPADALPWIFEPYRQAHGQQKGTGLGLAVVKGL
                     VEAQGGTVGVRSEAGKGSCFTVTLPKAPAAVGAQVAA"
     gene            17332..17685
                     /locus_tag="VFX87_02695"
     CDS             17332..17685
                     /locus_tag="VFX87_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993842.1"
                     /translation="MRAALAAYERVLREHPGDPAGASALHGLGRLQAGSTGGVRNYRA
                     AQRAYSRLLAEYPQSRWEADAHAWQVALGDLLAREAEATRLREEAARLKEEAVRLRTQ
                     IEQLRRTDLDLERRR"
     gene            complement(17693..17989)
                     /locus_tag="VFX87_02700"
     CDS             complement(17693..17989)
                     /locus_tag="VFX87_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993843.1"
                     /translation="MVRKGLTEREVQQALGDLDHADLPARTVAALRLADCLSGERPRV
                     DDALFAVLRQHFDEGQILELGAALTVASGWQRLIEAFGIRPDAWDETTPLPWPR"
BASE COUNT         2529 a         6603 c         6479 g         2542 t
ORIGIN      
        1 tccaccagcc ggcggcggaa catcggcagc aggtgcaggc ggccctcgat caggcggcgg
       61 acggcagcga agccgaaggc cttgggcgcg ctggcggggt cgatgacggt gaggccgccg
      121 acgtgcatcg gcaccgcgtt ggtctcgagg ttcaggaaca tcgtgtcgag accgctcaac
      181 tggcgcatcg acctctccac cctggaatgc cgcgcgattc tggcacacgc acgcgttaca
      241 ggtcgcgcag catctcccgc gccgcgttgc ggccggccgc gcccagcacg ccgccgcccg
      301 ggtgcgcggc ggcgccgcac aggtacaggc ccggcagcgg cgtgcggtag tcgctccagc
      361 ccggcacggg ccggaacgcg aacaactggt ggagcggcat cgcgccctgg aagatgttgc
      421 cgccggtgag gccgaacaca cgctcgatgt cgagcggggt cagcacctgg cgcgccagca
      481 ccgaccggct gaagttgggc gcgtactcgt cgagcagcgc gacgcaccgg tcggcgaagg
      541 cctcgccgat ctggtcccag ctcgtgcccc ggagcgcgta gggcgcgtac tgcacgaaga
      601 tcgacatcag gtggcggccg ggcggcgcga ccgtgggatc caccaccgag gggatcgtgc
      661 actcgagcac cggccgctcc gacgggcggc cgtacttcgc gtcgtcgtac gcgcgctcga
      721 tgtagtccag atcggggcag atgtggatgg tgccccgatg ctgggggccg gccgcggtgc
      781 cgggacgcgc ggtgaagctc ggcagctcgc cgagcgccag gttgatcttg agcgaggcgc
      841 tctcgtagga gatgcgcgcc accgcgtccc ggaagccggg cggcagcgtc tccggggcga
      901 gcaaccgcag gaaggtgacg tgggcgtcgg cgttgctcgc cacgatccgc gcgcggcatt
      961 cttccccgct ctcgagtacc acgccggtca cccggccgtc gcggaccgcg atgcgggcca
     1021 ccggcgcctc gcagcggatc tccacgccca ggtcgcgcgc cgcgcgcgcg agggcctggg
     1081 tcagcccgcc catgccgccg cgcacgtagc cccagacgcc gcggcggccg tcggtctcgc
     1141 ccatgacgtg atggaagagc acgtagccgg tgcccggcgt ggacgggctc gccatcgcgc
     1201 cgatgatcgc gtcggtcgcc agcgtggcct tgagtggggt ggactcgaac cagcggtcga
     1261 gaatcgtccg cgcgggcccg gtgagcagct cgaccgcctc ggaagcgtcg tcgcccagtc
     1321 gggcgaagcg gcggcccagg cccaggagct ggagcagccc gcgcgggccg gggtgcagca
     1381 cgtcgggcgg cgtcatgccg agcgcgggct cgatgaaggc ggccacccgc tccagcatgg
     1441 cctcgtactc cggataccgg gccgcgtcgc gcgcgctgaa cctggcgatc tcggtgcgcg
     1501 tgagctcggc gtccggcccc agggtgagcg agcggccgtc gggcagcggc gtgaaggacg
     1561 agggactgcg cggaagcacc tcgaagccgt agtcgcccag gcgcaggtcg cggacgatct
     1621 ccttgcggaa gaggctgttg acgtaggccg cggtcgatac cttgaagccg gggaaggtct
     1681 cctcggtcac gcaggcgccg cccaccagat accgccgctc gagcacgagc acgcgcagcc
     1741 ccgcgcgcgc caggtagcac gcggtgacga gcccgttgtg ccccgcgccg atcacgatcg
     1801 cgtcgtgctc attccctccg gcgcccgtca ctcgagcact tggtccgcgc ggagcagcag
     1861 ggactgcggg atcgtcagac ccagcgtctt cgcggtcttg agattgatga ccagctcgta
     1921 cttggtcggc tgctcgatgg gcaggtcgcc cgggcgcgcg cccttcagaa tgcggtccac
     1981 gtagaccggc acccgttgcc cccagagctc gacgatgttc ggcccgtagg ccatgaggcc
     2041 ccccgcctcc gccacctccc ggaagggcgc caccagcgga agccggtgct tgatcgcgga
     2101 atcggcgatc cgctgccgct cggcgaagaa cgcggagctc gacagcacga ggagtgcgtc
     2161 ggcgccttcg cgcttcaagg cgtcgagcgc gccgtcgtac tgagagggtt cctgcacctc
     2221 gaccacgtgc ggctggaggc ccagggagcg ggcggccgtc tcggccgccc ggacctgccc
     2281 caggccgccg gggcggggcc aggcgagcac ccccacgcgc cgcatgccgg ggagcacgac
     2341 tttgacgagc tcgaggcgct tgccggtcag ctcgggcgca tggagcgtca cgccggtgag
     2401 attgccgccg ggctgggcca ggctcgccac ccaaccctcc tcgaccggat catccaccgc
     2461 gaacgtcatg acggtcggga tcgtgcgggt ggccgcccgg gccgccggga tcgcgatgct
     2521 gctggccagg atgacgtcga cgccgagccc gaccagctcg gcggccagcg cgggcagcct
     2581 tgccaggtcc ccgcgcgcat agcgctgctc gatgatcaga ttctgccccg gcacccagcc
     2641 caggcgacgg atcccaaggc cgaacgctcc gatcccggtg ctcggacttc ccggcgagag
     2701 cagtccgacg cgatagaccc tcccggtccc ctgcgccgcg ccgcgacggg tcgaggccag
     2761 gaggcaaccc gaagcgaccg cgccgatgaa cgctcgccgg ttcattgcgt gccccgccgc
     2821 gatccgatac tgtctcgggc accatgacct tcgagccggt gaccccgcga ctcgagcgcc
     2881 tgtgccgggc gaccgtggag atcgccgcgc ccctcgtggt gggcgatacg ccgctcggcc
     2941 tgcggcgcgt catcccgatc accggcggcc gggtcgaggg cccgcgcctc tccggcgagg
     3001 tcctgccggg cggcgcggac tggcagatcg tgcgggccga cggcggcgcg gtgctggaag
     3061 cgcgctacac cgtgcggacc accgacggcg cgctcgtcta cgtgaggaac atcggtctgc
     3121 ggtgcgggcc gcccgaagtg ctggcgcggc tcggacgcgg tgagcatgtc gatccggcgt
     3181 cctactactt ccgcaccacg ccccgcttcg agaccggcgc cccggcccat gcctggctca
     3241 acgacgtgat cgcggtgggc tcggcggtac ggcgggccga cgcggtggtc ctggacttct
     3301 tcgtggtcgc ctagccggcc ggacaccaga tcggcgcgcg gctaccggat cacgcgcccc
     3361 gcgcggctca ccaccgcggt cgggatggtg atgccgagcg ccgccgcggt cttcaggttg
     3421 accgccagct ccaatcggct gggccgctcg acgggcagat ccgcgggctt ggcgcccttc
     3481 aggatcttgt ccacgtaggc cgcggcgcgg gtgttgacct cggccaggct cgggccgtag
     3541 gagagcaggc cgcccgcctc cacgatctcc ggccactcgt acatcgcggg caagcgatgg
     3601 cgggccgcga gctgcatgat ccgctcccgg ttgctcgaga ggatcgggtc ggtggtgacg
     3661 aagacggcgc gcgctccgcc gcgcatcatg ctcgcgaagg cgtcgggata ctgctcgggc
     3721 aggcgcgcgt cgaccacctg gatctccagc gcctgcgccc gcgccgccgc ctcggtctcc
     3781 cgcgccgccg ccgcgctgct cggattgcgg aggttggcga gcaccgcgac ccggcgcagg
     3841 ccgggcaggc actcgcggag cagctccagc cgcttgccgg ccagctccgg gaggtaggtg
     3901 gtcacgccgg tcacgttgct gccgggacgg gcgaggctgg ccacgaacag gcccgcgacc
     3961 ggatcggtgc cgctcaccat cacgatgggg atcgtggacg tcgcgttctc ggccgcccgg
     4021 atcgcgggcg aggacaccgc gacgatcaga tccaccccgc tccgcaccag gtcggcggcg
     4081 agatcgggca ggcgctcgaa gtcctcgttg gccgcgcgga tctcgaaccg gacgtcgcgg
     4141 ccttccacgt agcccaggtc gtgcaggcgc tggcgcagct cggggatgcc cccgcgggcc
     4201 gacaccgacg acggggtgag gtagccgacg cgggcaacgc gccccggctg ggcgggagcc
     4261 gcccccggga ggagcagggc ccccgcggcc agcgccgtga caaacgcccg ccggttcgcc
     4321 accgcgcccg ggctcatggt cgttggagtc tctcgctcat tcgtcgtaga atagccggac
     4381 ccgggaccac gtcaatagcc gcagccgcgg ggacagcatg ggacgagcgc gtatcctggt
     4441 agtcgacgac gagcgcgccg tgcgggacct gatctcggac gcgctgaaga tcgaaggcca
     4501 cgacgtcctc accgccagcg acggcaagga aggtctcgac ctcatcagcc agcaccgctt
     4561 cgatctcgtg ttctgcgacc tgcgcatgcc cgagatggac ggccaggccc tctacgagga
     4621 gatccagcgg aatcatccgc agatactcaa gcggatcgtc ttcgtcaccg cccaggcgaa
     4681 ctcggcagac tacgggccct tcctgcgcac caccgggatt ccggtcatcg agaagccctt
     4741 cacgctgagc cagctccgcc aggcggttac cagaatcgtg ggaccgatga gataggctgc
     4801 ggtggacggc ccccgcgccg tcaatcctgg ccgagcgact tcaggaaatc ggcgttggtc
     4861 gcggtgagct tgaggcggcc caggagcgct tccatcgcct cctgcggctt ggtgccggac
     4921 agggcgcgcc gcagcatatg gatcttcttc atgtcctcct cggagaacag cagctcctcg
     4981 cgccgcgtcc cggagcgcat aagatcgaac gagggaaaga tgcgccggtc ggccagctcg
     5041 cgcgagaggt agagctccat gttgccggtg cccttgaact cctcgaagat gatctggtcc
     5101 atccgcgagc cggtgtccac cagcacggtg ccgatgatcg tgagcgagcc gccctcctcg
     5161 atcttgcggg ccgccccgaa gaacgcgcgc ggcatctgga aggcgcggct gtcgatgccg
     5221 cccgacatgg tgcgcccgcg cgaggagagc tcgttgttgt aggcgcgggt catccgggtc
     5281 agcgagtcga agaggatcag cacgtcccgc ttctcctcca ccattcgctt cgctttgtcg
     5341 agtacctgct cggtgacttc gatatggcgg cggtagggat tgtcgttgct ggaggcgacg
     5401 atctccgcct gcttgccgat ctgcatcttg aagtcggtga cttcctcggg ccgctcgtcc
     5461 accaggagca cgatcaccac cacctcggga tggttggcga gcacggcctt ggcgatcttc
     5521 tggagcagga tggtcttgcc ggtgcggggc ggggccacga tcaggccacg ctgccctcga
     5581 ccgatcggcg cgatcatgtc cagcgcacgc atggagagct cgtcccgggt ggtctcgagc
     5641 ttcagcttct cgtacggctg gaccgcggtg aggccttcga agatggacgg gctgggaacc
     5701 cggggggcat ggcgcggagg catcgggcca cgcggcccct ggccttgccc tccgccctgg
     5761 ccttgccccg atccttgtcc ttgcccctgc ggcggtcggt tcccggagtg gcgccggtgg
     5821 cgggatcgcc tactcaagtg ggacgctcat tggcgcaaag ccctttccgt cagcaacatt
     5881 agaggagaac cgtcacgccg gtcaatcggc gccgcggtgc gggggcacgc gcggggggcc
     5941 ccgctccggg cgggcccatg actggcccaa gtgtaccgga acgcgttcgc cgtcaccaga
     6001 gaatcaggac ccggccggtt cgggcgttcc taccgggtcg cacgacacgc cgcacgccgg
     6061 cacgtgcgac cgaaggcgag acgcccctcc gcgagtcagc gcgccgcggc gggggactgg
     6121 gcgatgcccc cgcgcaccgc gccacccttg atgctgcccc agagcgtctc gaagccctcg
     6181 gggagcgggc ggctgttggg cggggccagc cagaggcgca tgagcagccg gtcgcggccg
     6241 ggctcggggg cgtcctcgta ggcggtgcgg ccgtggtaga tcacgtggtt gttcaggagc
     6301 tggatgtcgc cgggctcgag ctccatctcg tagcccagct cgtcgcagac cgcggccagc
     6361 aggtcgagcg cctcgtcctg ggccggggtg agccgcggga tcgtggcgat cttctgcgcc
     6421 gcctccacga aggtgcgcga gtactgggtg gtgaacttgc cctcgcgcat cgcgaagacg
     6481 ggcatcgcgt agatcgaggc ctccccgccc gcctcctcgc cctggcgggt gcggtagtag
     6541 tcggagcaga gcagcgcgtg gagatcgggc cgccgctcga ggatggcgtt gctcaccgcg
     6601 gggacgctga ccacgcggct ggtgccgccc cgacgggccg agcgcacgca gagcagcccc
     6661 acgacgtcgc agcggtccgt gtgccagcgc agcggcccgc tcgagcgcgc cttggcgcgc
     6721 gaggtcacgc cctcgctctt gtgcaccgcg ggctggacca cctcgccgta gagccggacc
     6781 tcgtcgcgca cctcgccgat cagctcgccg tgcgcgttct gatgcacggc cgttcccagg
     6841 tggcagccga ggccccacca gatcgcgcgc agcgcgtcgt ccgagtagcg cgccaccggc
     6901 aatccgcgga ggagcacgag gccgcgcccc cactcgagat cgcgattgac ctgcgccagc
     6961 tccgcggaga cgccgggcag cgggaagtcc ttcttggtga tgtcccgcca cacgaggccg
     7021 ccggcctgga ccgcgcgcag ggccgcgtcg atctcgtcca ccgcgcccgc cgagaggcgg
     7081 cggatccagt cggtcgaggc ggccagctcc cggccatgcc agaccatcgg gccagcgatc
     7141 ggcttgtcca tcgaagtccg cgtcatgggg gtcaaggcta gccgaggcgg cctcgcgtcc
     7201 gcaagcccgc gtggcttaga atcgcggaac catggcaggg ccgcgcggta ggctggtcgt
     7261 cgggctgctg ggaggcctgc tggcggcgct gctcgccgcc tgcgccggtc ccgcctcgga
     7321 tcgctccatg atcacgaccg cgcccaacgg cgcccgggag gagattccgg tcgcagtcag
     7381 caggcccgag ggcccgggcc cgttccccgc ggtggtgatc atgcacgact gcagcggcct
     7441 ggggccgcga tccagtggcg ccccggggcg ctgggcccgc gagctgggcg atcgaggcta
     7501 cgtgaccgtg atccccgaca gcttcacgac gcggggccat ccggacggcg tctgcaccga
     7561 cgcctcgccg agccggggcg aggtggcccc gagccggcgg gtgcgcgacg cgtacgcggc
     7621 gctcgcccac gcccgcacgc tcccgtacgt ggacggccgg cgcgtgggcc tgatgggcgg
     7681 ctcgcacggc ggctcgacca cgctcgcctc gctggccgcg ccgcagaacg cctgggagct
     7741 gctcgcccgc gagaagcgcg cgggcttcgc cgccgcggtg gcgctctatc cgagctgtgg
     7801 gaccaccctc ggcgcctggc gcgccgacgg caccggcacc taccgcccgg tggccccact
     7861 cctgatcctg atcggggaga aagatgactg gacgccggcc gcgtcatgcg tgaagctcgt
     7921 cgcgtccacg cgggcgacgg agcatccggc cgcgatcacc gtctacccgg gcgcgcatca
     7981 ctcgttcgac agcgaccggc cggtgcgctt cgtggccgcc cgcgtgaaca gcaacgcccc
     8041 cggcgggcgg ggcgccacca ccggcggcga cgccgccgcc tgggccgaca gcatccgccg
     8101 cgtggtcgag ttcttcgggg agcgcctcag gccgtaggag gagatggagg atgaccgggt
     8161 cggggtcctc gcgctgggca tggctcgccg ccctgaccgc ggcctatgtc gtcgccggca
     8221 agctcgggct gctcttcgcc tccgtccacg ccagcgcgac cgccgtctgg cctcccagcg
     8281 gcatcgctct cgccgcgctg ctcatcctgg gcgtccggat gtggcccgcg gtggccgccg
     8341 gagccttcct cgtcaacgtg accaccgcgg gctcggtcgc gacctcgctc ggcatcacgc
     8401 tgggcaatac gctcgaggcc gtgatcggct gctggctcgt cgggcggctc gcccacggcc
     8461 ggggcgccct cgagcggccg cgtcacatct tcgcgttctc ggccgcggtc gttccgggtg
     8521 cggccctcgc cgcgaccgtc ggcgtcgcga gcctcgtgct gggcggcttc gcgccgcggg
     8581 ccgacgcggc ggcgatctgg atcacctggg ctctcggcga tatcaccggg gcgctcatcg
     8641 tcgcgccgct cctgatcctg tggacggcgg accaccgctc cctggccctg cgccggcagc
     8701 cgatcgaggc gctcgcgttg ctcctaggcg tggtcggcgt cgccctgatc gtcttcggcg
     8761 ggctggctcc cgccgaatgg cgcgactatc cgctgacgtt cctggcgatc cccccgctgc
     8821 tctgggccgc catccgcttc ggccgccgcg aggcggcgac cgcggtggtc gtgctcatga
     8881 cgatcgcggt gggcggcacg atgcgggggt tcggggcgtt cgcggtcctg ccgccgaccc
     8941 actcgctgct cgtgctgcaa tcgttcgtgg ccacgatggc gctgatgacc ctcgtcgtcg
     9001 ccgcgctggt ccggagccgc gagcgggagg gcagcctgct ccaggccatc atcgaccgga
     9061 ttcccgtgat gatcacgatg tacgagccga acacccgcgc tctgcggctg aaccgggagt
     9121 tcgagcgcct caccggctgg tccagcgacg cggcgcggga cgtggacctg atggcgcgct
     9181 gctacccgga cccggcctac tgcgccgaga tccgcgccta catggactcg atgcgggagg
     9241 gctggcgcga cgtcgcgatg acgacgcggg acggtcgcgt cctgcagacc tcgtggtcga
     9301 acatccggct cccggacgat acccgcatcg gcatcggcct cgacgcgacg gagcgccggc
     9361 ggagcgagga cgagcgcgag cgcgcgcggg cggaagccga ggaggcgagc cggatcaagg
     9421 acgagttctt cgccatgctc ggccacgagc tgcgcaatcc gctcggcgcg atcacaaccg
     9481 cgctgcacgt gatcgacacc ggcgagccgg gggacgagcg gagcgcgcag gcccgcgaga
     9541 tcgtcacgcg gcaggtgcgc cacctcgtcc gcctcgtgga cgacctgctc gacgtgaccc
     9601 ggctcgccac cggcaagatc acgctcggcc ggcgcccggt gcacctcgcc gcgctcgcgc
     9661 ggcgcgtggt gggcgcgctg gccgcgacca cgccgactct gaacctccgc tccgaagccg
     9721 gggcggcggt gtggatcgag gccgacgaga cccgcctcga gcagatcctc accaacctcc
     9781 tcggcaatgc cgtgaagttc acgccggcgg gcggccgggt gacggtcgcc gtcgcggccc
     9841 ggggcggcga ggccgtgctc tccgtggagg acacgggcgc cgggatcccc gccgatctcc
     9901 tgccccggat cttcgacctg ttcgtgcagg gccagaccgg gctccaccgg tcggtgcccg
     9961 ggctcggcat cggcctcacc ctggtgaagc gtctcgtgga cctgcacggc ggtcgcatcg
    10021 aggcggcgag cgaggggccc ggccggggca gcgtgttcac ggtccgcttc ccgctccgcc
    10081 cggcccccca tgacgcggcg gacgcggccc cggtgcccgg cggcgcccgg gtccggcgcc
    10141 gcgtcctcat cgtcgaggac agcgacgacg cccgggacat gctgcggcac ctgctggagc
    10201 aggccgggca cgaggtgtac gaggccgccg acggcctcac cgggctcgac cgggccctcg
    10261 ccctcgagcc cgacgcggcg gtcatcgaca tcggcctgcc cgggctcgac ggctacgagg
    10321 tggcccggcg catcagggcc gccggccgcg cggacgcgct cctggtcgcg gtcaccggct
    10381 acgggcaggc cggcgaccgc cagcgctccg ccgaggccgg cttcgacgcc cacctcacga
    10441 agccggtgga tccgctcgcg ctcgagaccc tgctggtgaa cctgccgctt gacccccggc
    10501 gcacgggccg ctaggctcac cccaccatgc tctcgaccag cccccgcgtg accatcccgg
    10561 acgtgaccat caccgactac gtgctgcgcc atgccgcccg gctcggcgac aagcccgccc
    10621 tcgtcgacgg gcctaccggc cgcaccctca cctatcgtca gctcgccgac ggcgtgcggc
    10681 gcgcggcggc ggggctggcc aagcggggct tcggcaaggg cgacgtgctc gcgatctact
    10741 gcccgaacct ccccgagtac gccatcgtct tcctcggggt ggcgatggcg ggcggcgtca
    10801 acaccaccgt gaacccgctc tacaccgccg acgagctggc caaccagctc cgggactcgg
    10861 gcgcgcgctt tctggtcacg gtgcccccct tcctcgagaa ggcgaaggag ggcgcggcca
    10921 agagcagcgt cgaggagatc ttcgtcctgg gtgcggccga cggcgtccgg cccttcaccg
    10981 atctcatgca ggcgggcgat cagccgcccg cggtcacgat gaaccccgcc gaggatctcg
    11041 tcgcgctgcc gtactcgagc ggcacgaccg gcctgcccaa gggcgtgatg ctgacgcatc
    11101 gcaacctggt cgccaatctc tgccaggccg agggcatgcg gaacttcgag ggattcggtg
    11161 agcgggacat cacgatggcg gtgctgccgt tcttccacat ctacggcatg gtcgtgatcc
    11221 tgatgctcgg cctggccggc ggcggcacca tcctggtgat gccgcgcttc gacatgatgg
    11281 agttcctcac cttcgtgcag aagtaccgcg tgacgatcct gcccctggtg ccgcccatcg
    11341 tgctcgggct ggtgaagcac ccggcggtgg cccagttcga cctctcgagc gtgcgcctga
    11401 tcttctccgg cgcggccccg ctcggcgagg agatggcccg cgcgctcggc gccaagctcg
    11461 gctgcccggt ggtgcagggc tacggcatga ccgaggcgag cccggtcacc cacctgagcc
    11521 cgacccgcaa cgagccgttc aagccgggct cggccggcaa gatcgtgccc aacaccgagg
    11581 tgaagatcgt cgaggtgggc tcgggctcgg acgcggagct gccggtcggc aaggaaggcg
    11641 agctgtggat ccgcggtccc cagatcatga aggggtatct caaccgcccc gaggagaccg
    11701 cgggctgtct cgaccgcgac ggctggtacc acaccggcga cgtgggctac gtggacgacg
    11761 acggcttctt cttcatcgtg gaccgcacga aggagctgat caagtacaag ggcatgcagg
    11821 tggccccggc cgagctggag gcgctgctgc tcacgcatcc ggccatcctg gacgcggcgg
    11881 tggtgcgccg ggccgacgag gaggccggcg aggtgccgaa ggcctacgtg gtgctcaagc
    11941 ccgatgacgt ctcgaaggcc acgcccgcgg acgccatcat gggctgggtc gcccagcggg
    12001 tggccccgca caagcgcatc cgcgacctcg agttcatcga ccagatcccg aagtcggcct
    12061 ccggcaagat cctgcgccgg gtcctgatcg accgcgagaa gtcgcgctag gcgccggcgc
    12121 gacggccagg cgcgcccccc ggacgacgcg atgcccctcg cccaggggct cagctcccga
    12181 ctggccgcaa ggagaacgcg atgaacaaga tcgagcgagt gcgggcggcc ctggcgggcc
    12241 gtccggtgga ccgggccccc ttcaccgtct ggtatcactt cggcaaccag cacgctcccg
    12301 ccgagcgcac cgcgcaggtg catctggagt tcttcgacca ctacgacctg gacctgctca
    12361 agctcatgaa cgactacgac taccccatgc cggcgggcct ggaggtgatc gcgaccgcgg
    12421 ccgacctggg caagctccag cccttcgatc cggcccggac gccgttcggc acgcagctcc
    12481 agacggtgga gctggtggcc cgcgcccttc gcgaccgggc gctgttcgtg gacaccgtgt
    12541 tcaacgcctg gaacacgctc aagcgcaacc tggtgcgcga ggcgatgccc gcgctgatgg
    12601 tcgagcagcc gaaggcgctc gaggccgcgc tcgcggtggt caacgacaac ctgatccgtt
    12661 acgcccaggc gagcctggcc cgcggcgccg ccgggatctt cctctcggtg cccgccacca
    12721 ccgagagcct gacgctggac cagtacgagc gcttcatgcg tccgttcgat ctcgcgttct
    12781 tgaaagcgat cgagggccgt ggcgaatgtc acgtgctgca cgcgcacggc gagcacctct
    12841 acttcgaccg cctggtggac tatcccgccc acgcgctgtc gtgggccgac ctgaacggcg
    12901 gtcccagcat cgccgaggcc cggcggcgca cccggttcac gctgatggcg ggcctcgacc
    12961 acgtgaagtt cgtggagagc tcggcgcggc tggtgcgcgg gcaggtcacg cgggcgctgg
    13021 aggcgggcgg ggccaccggc ttcatcctcg cgccggggtg ctcgctgccg acgtatgcct
    13081 acccgccctt gatccgcgcc gcgcgggacg agtcgtcgaa gcagggcggt tcccgcttgg
    13141 gtgaatccca gggccacaag gggtaaatct ctacctcttg catcggttgc acactcgaaa
    13201 acgcctggaa aatagaggaa tttctgatgg aatggcgctt gctctcaccc tcgggcagga
    13261 ggagggcccg atgaccccga agctcatggc actggcagcc gtcgtcgtcc tgaccgtcgt
    13321 gttcctgagc ccggtcgcgt acatggcggg gcagcgcgcc accgtgtggc tcaccgcacc
    13381 cgcgggagag ccgaccggcc cctggccgag cgtggacgag gcggagcgcg aacccggccc
    13441 gaccgagcgc tccaggccga ccgttcgccc gggcttcggc gacatctagg ccctccggca
    13501 ggctcccgct cgcctacagg gtgaccacga gcccgtcgtg ggcgcactcc tcgggcaccg
    13561 agtccgcgcg ggccagcatc tcgctgctca tgtgggtgag aatcagccgc cgcgcgccga
    13621 cccggccggc gtgggccacg atgtcgggat agttcaggtg ccacttcacc ggcttgtcgt
    13681 agaagtagga ctccgcgatc agcagatcgc tgtcgtgcgc ggcgcgggcg acgtcgtcgg
    13741 tccattcggt gtcgccggtg tagcacatgg tcttgtcccc cacctggatc cgtagcgcgg
    13801 tgggattggt ctcctgggtg tggcgggcga gcgaaggggt caccaggaga tcgagcaccg
    13861 gctgggagcg gcccggctcc agctcgatcc agtgcaccgg gaacctcggc gtcatcacgt
    13921 gcgagccggg gaagagcgcc tcgcggatga cgtccatgcg cgcacggacc tcccgcgggc
    13981 ccgcgatggt gagggggcgg gcgcgccggg cccccagcat cgcgtccatg aggaggaacg
    14041 gcaccccggc ggcgtgatcg ccgtggagat gcgtgagcag gatcgcgtcg atcgagttgt
    14101 gctcgatccc ctgctgggcc agggcgatga gcgacgaggc gccgaagtcc accgcgaagc
    14161 ggcagcgcgg cccgtccacc aggatgcagg tctggaagcg ccctccactg ccgaaggaat
    14221 cgccggagcc cacgaagcgc acggtgacgg acacggctcc gatcttatgc gcccatgctc
    14281 ccatgcgaaa gccgtgacac gaaaaagctc ccgcgccgcg tcgcggcctg cgaggatagg
    14341 cgcgtgcggc tcttcgaccg gaagttcggc accgggttcc tggaagaggt ccccgccgcg
    14401 cccggcgtct accgcttcca cgacgccacc ggcgtcctct tctatgtcgg gaaggccgcg
    14461 aacctgcgcc gtcgcctcgg gcagtatcgc ctcgcgggac gccgcaagaa ggagcgcaag
    14521 cgccgcgcgc tcgtgaaggc ggcggcgcgg atcacctggg aggtctgcga gtcgccgctg
    14581 gccgccgccc tcgccgagat ccgcctcatc cagacgctcc ggccgccgca gaacgtggcg
    14641 agcgccttcc cgttcctcta cccctatgtc ggcgtgacaa ccgaaggcgc cgagacgtac
    14701 ttctgcctca ccaccgcgcc cgaggccttc ccggccttca cgttccacgg cgccttccgc
    14761 tcccgggagg tgaccggcga ggcgttcttc cgcctcatgg agctgctgcg ttacgtcggc
    14821 cacccggtgc cgcgccatcg ctgccgccgc ctcggcgcgg cccgccactc ctacgtgcgc
    14881 gggttccggc gcctgcccgc cgacagcgcg gagacgtgga gcgccctgct gcgcgggacc
    14941 tcgcgggagg cgctggaggc gctcgccctg cgactggtgg accacgccgg cgcgcgggcg
    15001 cggcgcgagc agacgcaccg tgccctccgc gcggtcgcgc gcttcttccg gcacgaggcc
    15061 cgggtgctgg cccgggtgcg gacggccacc ggctacccgc tctatcccgt gccccagcgg
    15121 gagcgcgacc tgctcttcgc ccgctaccgg gttgaggccc tgccgtgaca aggcttgtca
    15181 gggaggaggc gtctttcgta actcggtaag aacgctagcg tcgtaaggtg ctgtgcttac
    15241 acgccctccc gggtggcacc ttcagtgcac tgctctccgg catgccggcc gaaccctcgt
    15301 ccaggcgcat cctcgtcgtc gatgacgacg accagatccg tgatctcctg gccaccgccc
    15361 tgagcgccga cgggcacacg gtggacggag cccgggacgg cggcgaagcc ctggccctcc
    15421 tcgcgcacca cccgtatgac ctcgtgctca gcgacctgca cgtgccccgg atggacgggc
    15481 cctccctcta cgagacgctc cagacccgtc atcgcttccc ggtccggttc gccacgaagc
    15541 tgcctcgggt gatcttcatg accggcaatc ccgcggagca caccgagttc ctccgcggca
    15601 ccacggatcc gatcctggag aagccgttcg cgatgcgaat cgtccgcaag atggtccgcg
    15661 tgctcctttc cgccgccccc taggctcgtc cgcatgcgcc tcgccaccaa gatcttcctc
    15721 gtctcggcgc tcgtcatcct ggtcctctgc ggcacggtgg tgtggagcct gctcaccgtc
    15781 aagcggctcg tcagcgcgaa ccaggagatc gccacgcgcg cggtgccggc gctgcgcctg
    15841 cagggcgcgc tgcgcgaggc cgtgcagggg ctggtgcgcc tggaagcccg ggccctcgtg
    15901 ctccaggatc aggactacgc cgcgatgtgg agcgaccgcg ccacccgcgt gacccgcgac
    15961 ctggagaagc tcggctcgta tgtcgagcgc gccgaggagc ggaccgcgca ccggcgggcc
    16021 cgcgaagcct tcgcgacgta ttgcggccac gtcaaggagg agcgccggct cgtggcgctc
    16081 ggccagaccc gggcggcgct gcagatcgcg gagggccccg cgcgcgaggc ggcggagcgc
    16141 gcggaggccg cgctcatcga cgccaccgcc gcgaccgaag cggggctggc cgcgggccag
    16201 gcgcgcgccc gcgcgctcga agcccgcacc tggcgcgcgg tgaccgccgc gctggtggcg
    16261 agcctgctcc tcgcgctgtc ggccagcgcg gtgctcgcct accggatggc gcgatcgctc
    16321 ggccgtctgt ccgcggccac cacgtcgctc gcggccggca gctggaccgg gcccctcgtg
    16381 gtcacgggac gcgacgagat cagcgagctc ggccgcgcgt tcaaccagat ggccgagcgg
    16441 ttgagcgagg tggagcgcct caaggaagaa ttcttctcgc acatctcgca cgacctccgg
    16501 aacccgctgg ccgccatccg gctcagtgcc gaggccttgc aggagcgggc ccaggccgcg
    16561 ggcgacccga agcaggcgcg cttcgcgtac ctcatcgaca cgagcgccgc ccgcatgatg
    16621 gtcatgatca accagatcct cgacttcacg cggctgcggg cccgcaccgt accactggat
    16681 cgtaagccca ccgacctcct ccacgtcatc acccgcgcgc tggacgagct ccggccgatg
    16741 tccgaggaga agcggattcg ggtggacctg gcggcggagg gccacacctt cacggtgctg
    16801 ggggacgagg gcagcctggt gcgcgtggtc gtgaatcttc tcggcaatgc gctgcacttc
    16861 acgccgtccg ggggcgcggt gcgggcgcgc ctggccgagc tcccggaccg gctcgagctc
    16921 gacctcgagg acaccggtgt cggcattccc gccgacgcgc tgccctggat cttcgagccg
    16981 taccgccagg cccacgggca acagaagggc accggcctcg gcctggcggt ggtgaagggc
    17041 ctcgtcgagg cccagggcgg cacggtgggc gtgcggtcgg aggccggaaa aggaagctgc
    17101 ttcacggtga cgctcccgaa ggcccccgcg gcggtgggcg cgcaggtcgc cgcgtgaaac
    17161 cccacgcgcg gcgcctccgg tggatcgctc ctctcctgct gctcgcgctc tccgcgtgca
    17221 gcacggccag gccggccccg ggggggcagc gccgagccgc gcgatccggg tggctcgcac
    17281 gccgccgcgc agctcgtcgc ccaggccgac gacctcgcgc ggcgcggcca ggtgcgggcc
    17341 gccctcgccg cgtacgagcg cgtgctgcgc gagcatcccg gcgatccggc gggggcctcc
    17401 gcgctgcacg gtctcggccg cctgcaggcg ggttcgaccg gcggcgttcg caactaccgg
    17461 gccgcgcagc gcgcctactc gcgcctcctg gccgagtacc cgcagagccg ctgggaggcc
    17521 gacgcgcacg cgtggcaggt cgcactcggc gacctgctcg cccgcgaggc cgaggcgacg
    17581 cgcctccggg aggaggcggc gcgcctcaag gaagaggcgg tgcggctcag gacgcagatc
    17641 gagcagcttc ggcgcaccga cctcgatctc gagcggcgcc gctagccgcc gatcagcgcg
    17701 gccagggcag cggcgtcgtc tcgtcccacg cgtcgggccg gatgccgaac gcctcgatca
    17761 ggcgctgcca gccggacgcg acggtgagcg cggcgcccag ctccaggatc tgcccctcgt
    17821 cgaagtgctg ccggagcacc gcgaagagcg cgtcgtccac ccgcggccgc tccccgctca
    17881 ggcagtcggc gagccgcagc gccgccaccg tgcgggcggg cagatccgcg tggtcgagat
    17941 cgccgagcgc ctgctgtacc tcccgctcgg tcaggccctt gcgcaccaac gatgcgctgc
    18001 gcgccaggct tcagtgcacg cactggttgc gctgggcgag gcggatgcgg cagagctcca
    18061 ccagcgcggg gtcgaggctc gcgccgcccg cgtagatgaa gccgacccag gcgaggaaca
    18121 gatcgagcac cgcgggccgg cgcgcgagcg tga
//
LOCUS       DASUFA010000070         5625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_236190, whole genome shotgun sequence.
ACCESSION   DASUFA010000070 DASUFA010000000
VERSION     DASUFA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236190"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFX87_02705"
     CDS             complement(<1..552)
                     /locus_tag="VFX87_02705"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HEX4993844.1"
                     /translation="MPPSGTLRRPTFHGAGGTLFGIHVVNTLLTLVTLGFYYFWAKTR
                     LRRYLFGQTEIEGDRFAYHGTGRELLLGTLKAVLVFGVPIYLLNIIRDVLDVPAPVKV
                     GAGLLSASLLFVLFPVAMVGARRYRLGRTSWRGIRFSFRGRVWELMKIVIVGSFLTGL
                     TLGLYYPILLVKRQAFMISNSYFG"
     gene            complement(779..1924)
                     /locus_tag="VFX87_02710"
     CDS             complement(779..1924)
                     /locus_tag="VFX87_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4993845.1"
                     /translation="MNFDLTEEQQLLADSLKRYLTNEYSIDARAKIVDSPGGWSEAVW
                     AAFAEMGLLGVPFAEEHGGFGGTAVDVMLVMEALGEGLVVEPYWVNVGLGGRLIARAG
                     SVAQQKRILPALIQGKHRVAFAQTERGARYDLGHVGTRAKRGGGGFTLNGEKRAVLHG
                     GSADTLIVSARTAGSDTDAAGISLFLVDRGAKGVTLKEYRTIDELRAADVWLTDVAVP
                     ADALLGEEGRALPLIEDAADYATALLCAEAVGAIRYANEATLEYLKTRRQFGVPIGSF
                     QALQHRMVDMVISYEQARSMACLACVKVDSAPPVERARAVSAAKIKVADACRHVSQEA
                     VQLHGGMGMTEELKISHTFRRLTMIGQLFGDAEHHLERFAASDGGGA"
     gene            complement(1921..3102)
                     /locus_tag="VFX87_02715"
     CDS             complement(1921..3102)
                     /locus_tag="VFX87_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4993846.1"
                     /translation="MDLNYSPDELAFRDEVRGWLRSNLPDELRAKMEGYQDLSKDDLL
                     RWHKILAKKGWVAPDWPKEWGGTDWNVVQRYIWEEECGFAATPPLIPFGLRMCAPVLL
                     QFGTEAQKRKFLPRIYQGEDFWCQGYSEPGSGSDLASLKTRAVRQGDEYIVTGQKIWT
                     TLAHHADWIFCLVRTDGSTGKRQDGISFLLIDMHTPGITVRPIVLLDGSKEVNEVFFD
                     EVKVPAENLVHEEGKGWTVAKYLLGYERMGTGRIGASKRELTKLKELAGEQMKDGRPL
                     MEDSRFRDRLTRVEVELIALEITNLRFLDQMRRSGRPPGADVSMLKIKGTEIQQAITE
                     LMMQAAGPLAQAFRPVDAMDFDHFTARLAPRYCNMRKATIYAGSNEIQRNIIAKATLG
                     L"
     gene            complement(3156..4190)
                     /locus_tag="VFX87_02720"
     CDS             complement(3156..4190)
                     /locus_tag="VFX87_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4993847.1"
                     /translation="MRVSISLRLAQTDWAEASSYVTEAERLGVDDVWSAEAWGHDGVT
                     PLAFLAARTSRIRLGTGILQAGTRTPALLAMTAMSLQAMSNGRFVLGLGVSGPQVIEG
                     WHGIRFDRPVQRMRETIEIVRRAVKGERLEYKGQVYELPLPGGEGKALRSAAPPQPTL
                     PVYLATLSPKSLEMTGEIADGWLGTSFMPAHARVFTDSLAAGAARAGRSLAQLDLQAG
                     GFVAFGDDVERLIAPRKPGLAFSLGAMGSRQHNFYNDAYRRAGYVDAAAEVQRLWLEG
                     RREEATARVPDELVLQTNLLGTDAMVRARLAAYQRAGITTLRVEPGGEGLAARLATLG
                     RLLDLARALR"
     gene            complement(4190..4714)
                     /locus_tag="VFX87_02725"
     CDS             complement(4190..4714)
                     /locus_tag="VFX87_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4993848.1"
                     /translation="MNEEQARIRGYLVAQGAKLTPAAIVEKVQQAMADLKAAAAAVPA
                     ARFGERPAPEEWSGNEVMAHVVAADGYFGGGIQRLLAGQPPPARPGGRGVEGAPLLSA
                     EAWYRTLAQQRDALFAAVLAADPAAAPDQRIEHPAFGPLTWRETLLFTRLHDLDHAGQ
                     LQKIATALAAPPPG"
     gene            complement(4846..>5625)
                     /locus_tag="VFX87_02730"
     CDS             complement(4846..>5625)
                     /locus_tag="VFX87_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4993849.1"
                     /translation="AADPVAAPVVVWIHGGSVEDSSVMVADLEPFFDRMRALFPDTRG
                     HGLSSRFERVEDYTYARKAEDLLAWLDALGIREAVWGGVSMGGALSLWIAAHAPERAR
                     AVISISGPPFVPSDEDKAWWARQRPLVAEGRFEEYFDANVRLRMGEEALARLKARPER
                     YAELTGALRRHSVASLLALLDETYRRGEWLDDCRHIRCPVQVIAGSDDSFPTVAMSRR
                     VAEAIPDARLHVVQGGPHFPNRTHRAEVQAVIAAFLTTVGV"
BASE COUNT          810 a         2030 c         1980 g          805 t
ORIGIN      
        1 cccgaagtac gagttcgaga tcatgaacgc ctgccgcttc accaggagga tcgggtaata
       61 gaggcccagg gtgagcccgg tgagaaagct accgacgatg acgatcttca tcagctccca
      121 cacgcgcccc cggaacgaga agcggatccc gcgccacgag gtccggccga gccggtagcg
      181 gcgcgccccc accatcgcga ccgggaagag cacgaagagc aggctggccg agagcagccc
      241 ggccccgacc ttgacggggg ccggcacgtc cagcacgtcg cggatgatgt tgagcaggta
      301 gatcggcacg ccgaagacga ggaccgcctt gagcgtgccc agaagcagct cccgccccgt
      361 gccgtgatag gcgaagcggt cgccctcgat ctcggtctgc ccgaagaggt accgccgcag
      421 gcgcgtcttg gcccagaagt agtagaagcc gagcgtgacg agggtcagca gggtgttgac
      481 gacgtggatg ccgaagagcg tgccgccggc gccgtggaag gtcgggcgcc gcagcgtgcc
      541 ggagggcggc acgggctggc gctcgggagg cggcgggggg gcgggtggag gcgcggcggg
      601 aggcggcggc gcgatcggcc ggcgaggagc ggggctcccg gcggccgcgg gtttgcggaa
      661 gaacgacggc cgcccctcgg gatcctgccg gtccaccata cccttcgact atgggcggaa
      721 gcggagcggc gagtcaactt ttgccagtca gctctcgcca ccaaccgact ctcgaaggtc
      781 aggcgccccc gccgtcgctg gccgcgaacc gctcgaggtg atgctcggcg tcgccgaaga
      841 gctggccgat catggtgagc cgccggaacg tgtggctgat cttcagctcc tcggtcatgc
      901 ccataccgcc gtggagctgg accgcctcct ggctcacgtg ccggcaggcg tccgccactt
      961 tgatcttggc cgcggagacc gcgcgcgcac gctccacggg cggcgcgctg tccaccttca
     1021 cgcacgccag gcatgccatg gaccgcgcct gctcgtagct gatcaccatg tccaccatgc
     1081 ggtgctggag cgcctggaag ctgccgatcg gcaccccgaa ctgccgacgc gtcttgaggt
     1141 actcgagcgt ggcctcgttg gcgtagcgga tggcgcccac cgcctccgcg cagagcagcg
     1201 cggtggcgta gtcggcggcg tcctcgatca gcggcagcgc gcggccctcc tcgcccagga
     1261 gggcgtcggc gggcaccgcg acgtcggtga gccagacgtc ggcggcgcgt agctcgtcga
     1321 tcgtccggta ctccttgagg gtcacgccct tcgcgccgcg gtccaccagg aacaggctga
     1381 tccccgccgc gtcggtgtcg cttcccgcgg tgcgcgcgga gacgatcagg gtgtcggcgg
     1441 agccgccgtg gagcaccgcg cgcttctcgc cgttcaacgt aaagcctcca ccaccgcgct
     1501 tggcccgcgt gcccacgtgg ccgaggtcgt agcgggcacc gcgctcggtc tgggcgaagg
     1561 ccacgcgatg cttgccctgg atcagcgcgg gcaggatgcg cttctgctgg gccaccgagc
     1621 cggcgcgggc gatcaggcgg ccgcccagac ccacgttgac ccagtagggc tcgaccacga
     1681 gcccctcgcc gagcgcctcc atgaccagca tcacgtccac cgcggtaccg ccgaagccgc
     1741 cgtgctcctc cgcgaacggc acgccgagca ggcccatctc ggcgaacgcc gcccacaccg
     1801 cctcgctcca gccgccgggt gagtccacga tcttggcccg cgcgtcgatc gagtactcgt
     1861 tcgtcaggta ccgcttgagg gagtcggcca ggagctgctg ctcttcggtc aggtcgaagt
     1921 tcatagcccg agcgtcgcct tggcgatgat gttgcgctgg atctcgttgg agcccgcgta
     1981 gatcgtcgcc ttcctcatgt tgcagtaccg gggcgccagg cgggccgtga agtggtcgaa
     2041 gtccatcgcg tccaccgggc ggaaggcctg ggccagcggg cccgcggcct gcatcatcag
     2101 ctcggtgatc gcctgctgga tctcggtgcc cttgatcttg agcatggaga cgtcggcgcc
     2161 cgggggccgc ccgctccgcc gcatctggtc gaggaagcgc aggttggtga tctcgagcgc
     2221 tatcagctcg acctccacgc gcgtgagccg gtcgcggaag cgggagtcct ccatgagcgg
     2281 acgcccgtcc ttcatctgct cgcccgccag ctccttcagc ttggtcagct cgcgcttgga
     2341 ggcgccgatg cggccggtcc ccatgcgctc gtagcccagc aggtacttgg ccaccgtcca
     2401 gcccttgccc tcctcgtgca cgaggttctc ggcgggcacc ttcacctcgt cgaagaagac
     2461 ctcgttgacc tccttgctgc cgtccagcag gacgatcggg cgcaccgtga tgcccggggt
     2521 gtgcatgtcg atcagcagga aggagatgcc gtcctggcgc ttgccggtgc tgccgtcggt
     2581 ccgcaccagg cagaagatcc agtcggcgtg gtgcgcgagc gtggtccaga tcttctggcc
     2641 ggtgacgatg tactcgtcgc cctggcgcac cgcgcgcgtc ttcagcgagg ccaggtccga
     2701 gcccgagccc ggctccgagt agccctggca ccagaagtcc tcgccctggt agatccgggg
     2761 gaggaacttc ctcttctgcg cctcggtccc gaactgcagc agcaccggcg cgcacatccg
     2821 gagcccgaac gggatgagcg gcggcgtggc cgcgaagcca cactcctcct cccagatgta
     2881 gcgctgcacc acgttccagt cggtgccgcc ccactccttc ggccagtccg gcgcgaccca
     2941 ccccttcttg gccaggatct tgtgccagcg caggagatcg tctttcgaca gatcctggta
     3001 gccttccatc ttggcgcgca gctcatccgg caggttgctc cgcagccagc cgcgcacctc
     3061 gtcacggaac gccagctcgt cgggcgaata gttcagatcc atgtcagggc tcctgtggaa
     3121 tcgtcgttga tcgcgcgtcc cggccgaacg ccatgctatc gcagggcgcg agcgaggtcc
     3181 agcaaccggc ccagggtggc gagccgggcc gcgagcccct cgccgcccgg ctcgacccgc
     3241 agcgtggtga tgccggcgcg ctggtaggcg gcaagccgcg cgcgcaccat ggcgtcagtg
     3301 ccgagcaggt tggtctggag caccagctcg tcgggcacgc gggccgtcgc ctcctcccgc
     3361 cggccctcga gccacaggcg ctggacctcg gcggccgcgt ccacgtagcc ggcccgacgg
     3421 taggcgtcgt tgtagaagtt gtgctggcgc gagcccatcg cgcccaggct gaaggccagg
     3481 ccgggcttgc gcggcgcgat cagccgctcc acgtcgtccc cgaaggccac gaagccgccg
     3541 gcctgcagat cgagctgggc gagcgagcgg cccgcgcggg ccgcccccgc ggccagcgag
     3601 tccgtgaaga cgcgcgcgtg cgcgggcatg aacgaggtgc cgagccagcc gtcggcgatc
     3661 tcgcccgtca tctcgaggct cttcggcgag agcgtggcca gatagaccgg cagggtcggc
     3721 tggggcggcg cggccgagcg gagcgccttc ccctcgccac cgggcagcgg cagctcgtag
     3781 acctggccct tgtactcgag gcgctcgccc ttcaccgcgc ggcgcacgat ctcgatcgtc
     3841 tcgcgcatgc gttggaccgg ccggtcgaag cgaatgccgt gccagccctc gatcacctgt
     3901 ggcccgctca cgcccaggcc cagcacgaag cggccgttgg acatggcctg caggctcatc
     3961 gcggtcatgg cgagcagcgc gggcgtgcgg gtacccgctt gcagaatccc ggtgccgagg
     4021 cggatgcgcg aggtgcgcgc ggccaagaag gccagcgggg tgacgccgtc gtgcccccag
     4081 gcctccgcgg accagacgtc gtccacaccc aggcgctcgg cctcggtcac gtagctgctc
     4141 gcctccgccc agtcggtctg ggcgagccgt agcgagatcg agacgcgcat cagccgggcg
     4201 gcggcgccgc gagggccgtc gcgatcttct ggagctggcc ggcgtggtcg agatcgtgca
     4261 gccgggtgaa gagcagcgtc tcgcgccagg tgagcggccc gaacgccggg tgttcgatcc
     4321 gctggtcggg cgcggccgcg ggatcggcgg ccaggacggc cgcgaacagg gcgtcgcgct
     4381 gctgcgcgag cgtccggtac caggcctcgg ccgagagcag cggcgcgccc tcgacgcccc
     4441 gcccgcccgg gcgcgccggg ggcggctggc ccgcgagcag ccgctggatg ccgccgccga
     4501 agtagccgtc ggcagccacc acgtgggcca tcacctcgtt gccgctccac tcctcgggcg
     4561 cgggccgctc gccgaagcgc gccgcgggca ccgcggccgc agccgccttc aggtccgcca
     4621 tggcctgctg caccttctcc acgatggccg ccggggtcag cttggccccc tgggcgacca
     4681 ggtacccgcg gatccgcgcc tgctcctcgt tcatggcggc ttggactcta tccctccccc
     4741 gcccgccccg caatggccgg ggtcaggtct tacattccaa catttccggg cgcgtgtgcc
     4801 ccacctggcc gatagaaaat gttggactgt aagacctgac cccgcttaga cgccgaccgt
     4861 agtgaggaag gcggcgatga cggcctggac ctcggcgcgg tgggtgcggt tggggaagtg
     4921 gggaccgccc tggaccacgt ggaggcgggc gtccgggatg gcctcggcga cgcgccggga
     4981 catcgccacg gtcgggaagc tgtcgtccga gcccgcgatc acctggaccg ggcagcggat
     5041 gtgccggcaa tcgtccagcc actcgccgcg gcggtaggtc tcgtccagca gcgcgagcag
     5101 ggaggccacc gagtggcggc gcagcgcgcc ggtcagctcc gcgtaccgct cgggccgcgc
     5161 cttgagccgg gccagcgcct cctcgcccat gcgcaggcgc acgttggcgt cgaagtactc
     5221 ctcgaagcgg ccctccgcca ccagcggccg ctgccgcgcc caccaggcct tgtcctcgtc
     5281 gctcggcacg aagggcggcc cgctgatgga gatgaccgct cgcgcccgct ccggggcgtg
     5341 cgccgcgatc cacagcgaga gcgcgccgcc catggacacc ccgccccaca ccgcctcgcg
     5401 gataccgagc gcgtcgagcc acgccagcag gtcctcggcc ttgcgcgcgt aggtgtagtc
     5461 ctccacccgc tcgaagcgcg aggagaggcc gtggccgcgc gtgtcgggga aaagcgcgcg
     5521 catccggtcg aagaagggct cgagatccgc caccatcacc gaggagtcct cgaccgagcc
     5581 cccgtggatc cacaccacca ccggagcagc aacggggtcc gcggc
//
LOCUS       DASUFA010000071        10493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1611842, whole genome shotgun sequence.
ACCESSION   DASUFA010000071 DASUFA010000000
VERSION     DASUFA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611842"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFX87_02735"
     CDS             <1..292
                     /locus_tag="VFX87_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993850.1"
                     /translation="WPLQVGRSWSATVTWVDGPAQDQRFRLTGVWTVEAYEEVKTPAG
                     TFKAFKVSRREIESGASQEFWYSPAVKGWVKVRGTDTAAGAYEEELTAYTAR"
     gene            complement(300..1115)
                     /locus_tag="VFX87_02740"
     CDS             complement(300..1115)
                     /locus_tag="VFX87_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4993851.1"
                     /translation="MTRERLRVLAWQLLILVAVLAAWQYLTGIKAISKTPGLYWIDPF
                     FISRPSAILERLLYLMSPRVRLTIWQMALSTVQSTVWGFLVGISTGFVAGLVLGRSDG
                     LARIFQPYIVAFNSLPRIALVPLITMIFGFGLLAKIVLAWSIVFFIVFFNTFQGARSV
                     DADLIHSARFLGASERQIMTSVVVPSALAWTFASLTPSISFALIGVVVGEFIGGESGG
                     GLGYLIIQSLGTLNAADMMVALFVLGAIGIVMALGIKQVEARLLRWRPEYQKR"
     gene            complement(1112..1876)
                     /locus_tag="VFX87_02745"
     CDS             complement(1112..1876)
                     /locus_tag="VFX87_02745"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4993852.1"
                     /translation="MRDLAKTFGQGPREVRAVSPVSFDVHEREFVAIVGPSGCGKSTI
                     LNMIGGLVAPSGGEIVVDGQRVTGAPPPQVGYVFQKDTVFPWRTVERNIALGLEYRNV
                     PAEERAARVRQAIALAGLQGFEDAFPATLSGGMRQRVALMRSLVVDPEILLMDEPFGA
                     LDTHTKLNLHAELLEIWQAKRQTVIFVTHDLSEAITLADRIVVMTRRPGRIKLIYDVR
                     LPRPRDVIRIRESDEYSREYSEVWHALGEEFRAVAP"
     gene            complement(1911..3005)
                     /locus_tag="VFX87_02750"
     CDS             complement(1911..3005)
                     /locus_tag="VFX87_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993853.1"
                     /translation="MAEQPQPARWTRREFLKSTAVAGGLAVGGSRSGRAWAQGLKSIS
                     ASHSVSTFVYGQHLVAAQKKLFEEEGVKTPDFVVPGGGAKVVQALAAGQVLFALGDSN
                     HPLKITEKGKDALMVFATDTRCSYANIVVRKELWDKGVKTVEALADQKLVGRKAVVAA
                     TAIGSGTYVYGVYVLQNLKGGDGKPVNDHVEWVGGGASTTMLGGLKAGKFDAIMAVPE
                     WQSAAVEEGFGKPLYDIQDEKAWNRVFGGPIPVTVGYVLKETIEKSPDLVQAYVNACY
                     RAQQWIRRAKDDEITDLLWKPYMDTFKREVVLESVRYYRTIFDWDFVIEERDYERGMK
                     VWVPTAVEKPIPFKQAVDMSFVKKAQAKYK"
     gene            3184..4068
                     /locus_tag="VFX87_02755"
     CDS             3184..4068
                     /locus_tag="VFX87_02755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX4993854.1"
                     /translation="MIRSMTGYGRAEAAGARTILSVECKSVNHRHLDVSLKLPRVLGA
                     FEADARRLIQAAVQRGRVDVSATVTAPEGTVLNPLTVNLAQAKEYAEAARALAQALDL
                     GDAPSLGWVMGQSGVLTREEQAPLSAEEAWPLLERSLSAALAELVERRETEGRALGQE
                     LAGLGATLAAHVDTVAQRAPMAVERRAVRLRERMQAMLAGAEIDQSRLATEVAVWADK
                     TDVSEEVARLRAHLTQLGALLAADGPVGRTLDFLIQEINREVNTIGSKADDLEISQAV
                     IAAKSTLEKMREQVANLE"
     gene            4134..4745
                     /gene="gmk"
                     /locus_tag="VFX87_02760"
     CDS             4134..4745
                     /gene="gmk"
                     /locus_tag="VFX87_02760"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX4993855.1"
                     /translation="MKRRGALVVVSAPSGAGKTTLCHEVRSLVPELYYSVSYTTRDPR
                     PGEKEGTDFFFVSEGRFTAMRGRDEFAEWAQVHGHHYGTPAKALEGALSRGLDVLLDI
                     DTHGARQLRQRYPEAVSVFIMAPSMAELEARLRERKSDSAGDIARRLSRAREEIAAWR
                     QYDYLIINRDVKDAVDQLATIIQAERCRTSRLTLRFPDMEVPA"
     gene            4745..4957
                     /gene="rpoZ"
                     /locus_tag="VFX87_02765"
     CDS             4745..4957
                     /gene="rpoZ"
                     /locus_tag="VFX87_02765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX4993856.1"
                     /translation="MSFPSLEKSLDNVSNRYMLVVLAAKRARQLNRGAKSQVESRHKK
                     PTSNALEEIAESRVGYRVKEEDAPKA"
     gene            5099..6334
                     /gene="coaBC"
                     /locus_tag="VFX87_02770"
     CDS             5099..6334
                     /gene="coaBC"
                     /locus_tag="VFX87_02770"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX4993857.1"
                     /translation="MTLHDKELILGVTGSIAAYKAVFLLRELTRAGAQVTVVTTAHAE
                     RFVGALTFRTLSGRPVLSDLFDPRSAEAVEHVALAERAQAMIVAPATANLLAKAAHGI
                     ADDFLTTLLLAARCPVLMAPAMDIGMWDHAAVTANVLTLRGRGVTVLEPDAGALASGL
                     SGRGRLPEADAILEALERLLAPRRDLVGERVLVTSGPTREPIDPVRYISNRSSGKMGQ
                     AVATAALRRGAEVVLISGPTTLTPPAGAVYVPVQTAEDMREAALQHLERATIVIKAAA
                     VADYRVKQPSAAKIKSRKDEGLTLDLAPNPDILKELAARKGGAFLVGFAAETNEVRAN
                     AAAKLRAKGVDLLVANDVSVKGIGFDAEDNQVLLLDRWGGAMDLPKMSKLDVADAILD
                     RVVGLRAADRSPASNRATR"
     gene            6339..7037
                     /locus_tag="VFX87_02775"
     CDS             6339..7037
                     /locus_tag="VFX87_02775"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX4993858.1"
                     /translation="MSDPREAVALALRGLADTLRHHRDLGLRDLALSTVPFPDPADAL
                     REQERARQGCQLCKLCKSRTNVAFGSGNARARLMVVGEGPGEEEDKQGLPFVGRAGQL
                     LTKMLASVGFDRERDCYIANVVKCRPERNRNPEPDEVAACSPFLMAQIDTIGPHVILA
                     LGNFAAQTLLGTKEGITRLRGKTYQYRASVLVPTFHPAFLLRNPGPEFRRMAWDDLKL
                     ARREYDARSGDGPR"
     gene            7105..8871
                     /locus_tag="VFX87_02780"
     CDS             7105..8871
                     /locus_tag="VFX87_02780"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993859.1"
                     /translation="MIADVVFDIPIDHAFSYVVPPELVVARGQRVGAPLHGRARVGVV
                     VGLREGDPAGLKPLQRAVEPVPVLSEAALELGRWAAAESLSSWGSTLLSLLPPPPRGG
                     AAEAVSPPAAPAPGPPRAPELWTDERREDRLVECLEREPGATLLIAPDREAAARWARR
                     LDAPRLDSGVTEAERRRAWFAASRGRSRVVVGTRSALLAPLPPPATLALIDEHDPAHK
                     PPGAPRLHSRDLLLRRAELDGSRLLLLSATPSAESWWHARQGRIVRPDAPAAPWPQVI
                     TADTRGILRNHPLTLPLTRAIEDMSRQGRRAVLIVSRRTAALACDECGAVLRCPDCGV
                     ALALARDRSALRCPLCARAETPPDHCPACGGHRLSPFGWDPERVQTSIARRFPRLTIS
                     RQSLEAQVLIGTPALLRALPRRSVGCVGFVALDGLLRVPDFRAGERTWQLLWAAAESV
                     VPGGRLVIQTQHPEHYAIRAVQAQDRASFYDEELKFRAELGYPPYRRLAEVSARGRED
                     GPVRALAAQCAAALRGIGGLTVYPPASVTPPRGRNSRWRFVIKGPAELPRLLAPALEP
                     FLGQRRRSSDMVEIEMDPQSNG"
     gene            8900..9421
                     /gene="def"
                     /locus_tag="VFX87_02785"
     CDS             8900..9421
                     /gene="def"
                     /locus_tag="VFX87_02785"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008222299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX4993860.1"
                     /translation="MALLTVRLYGDPALRQVAAPVRDITAEIKRIIADMTETMWHQVG
                     IGLAAPQVGLPYRILVMDDGKGGAQTLINPEIETRSGTVREEEGCLSLPGIFGVVERS
                     KTITVRAMDADGKPVSLEATGLKARIIQHELDHLDGVLFIDRLPPVTRDRIKKKIQKE
                     GFPKETGNHAFAL"
     gene            9441..10364
                     /gene="fmt"
                     /locus_tag="VFX87_02790"
     CDS             9441..10364
                     /gene="fmt"
                     /locus_tag="VFX87_02790"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX4993861.1"
                     /translation="MFYGTPAFALPTLEALLARHQVVAVITQPDKPAGRGRAVTASPV
                     KRRALVASVPVLQPPRLRDAGWPERLAEYRADVAVVAAFGQILPKAVLDVPVHGSINV
                     HGSVLPRYRGAAPIAWAIMRGETETGITTFQMDPGMDTGDMLLTEATPIGPEETAGEL
                     SVRLAEIGARLLLRTLDGIDTLPRIPQDHAQATLAPRLKKEDGWLRPAEPARDLVNRV
                     RGCNPWPGAALITPAGRLLIWRATAVPAPGVTAPSGSLVASEGALAIATGDGLFLPLE
                     VQPENRKAMRWADFLRGARLAAGARVSEPTP"
BASE COUNT         1578 a         3782 c         3653 g         1480 t
ORIGIN      
        1 gtggccgctc caggtcggcc ggtcgtggtc ggccaccgtg acgtgggtgg acgggcccgc
       61 gcaggaccag cgcttccgcc tcaccggggt ctggacggtg gaggcctacg aggaggtcaa
      121 gacgcccgcg ggcaccttca aggcgttcaa ggtgtcgcgg cgcgagatcg agagcggcgc
      181 gtcgcaggag ttctggtaca gcccggcggt gaagggctgg gtgaaggtgc gcggcaccga
      241 taccgccgcg ggcgcctacg aggaagagct gaccgcctac accgcgcgct gaccgacggt
      301 cagcgcttct ggtactccgg ccgccagcgc agcagccgcg cctcgacctg cttgatcccg
      361 agtgccatca cgatcccgat cgcgcccagc acgaagagcg ccaccatcat gtcggccgcg
      421 ttcagggtgc cgagggactg gatgatcagg tagccgagcc cgccgcccga ttcgccgccg
      481 atgaactcgc cgaccaccac cccgatgagg gcgaaggaga tggacggcgt caaggacgcg
      541 aaggtccacg cgagcgcgga gggcacgacc acgctggtca tgatctggcg ctcggaggcg
      601 cccaggaagc gggcggagtg gatgagatcg gcgtccacgc tgcgcgctcc ctgaaaggtg
      661 ttgaagaaca cgatgaagaa cacgatgctc caggccagta cgatcttggc gagcaggccg
      721 aagccgaaga tcatcgtgat gagcggcacc agcgcgatgc gcgggagcga gttgaaggcc
      781 acgatgtagg gctggaagat ccgcgccagc ccatcgctcc gtcccaggac caagcccgcc
      841 acgaagccgg tggagatgcc gaccaggaag ccccacacgg tggactgcac ggtggagagc
      901 gccatctgcc agatggtcag ccgcaccctg ggcgacatga ggtagaggag acgctccagg
      961 atcgcagacg gccggctgat gaagaacggg tcgatccagt agaggccggg ggtcttcgag
     1021 atcgccttga tcccggtcag gtactgccag gcggccagga ccgcgaccag gatcaggagc
     1081 tgccacgcga ggacgcggag gcgttcgcgg gtcacggcgc caccgcccgg aattcttcgc
     1141 cgagcgcatg ccagacctcc gaatactcgc gggagtactc gtcgctctcc cggatgcgga
     1201 tcacgtcgcg ggggcgcggg agcctcacgt cgtagatcag cttgatgcga ccgggccggc
     1261 gcgtcatcac cacgatgcgg tcggccaggg tgatcgcctc ggagagatcg tgggtcacga
     1321 agatcacggt ctgccgcttc gcctgccaga tctcgagcag ctcggcgtgc aggttcagtt
     1381 tggtgtgggt gtcgagcgcg ccgaagggct cgtccatcag caagatctcg gggtccacca
     1441 ccagggagcg catcagggcc acccgctgcc gcatgccgcc ggagagcgtg gccgggaagg
     1501 cgtcctcgaa gccctgaagc ccggccagcg cgatggcctg gcgcacgcgg gcggcccgct
     1561 cctcggccgg cacgttgcgg tattcgaggc cgagcgcgat gttgcgctcg accgtccgcc
     1621 agggaaagac ggtgtccttc tggaagacgt agccgacctg gggcggcggc gcgccggtga
     1681 cccgctggcc gtccaccacg atctccccgc cggacggcgc gaccaggccg ccgatcatgt
     1741 tgaggatggt ggacttgccg cagccggagg ggcccacgat cgccacgaac tcgcgctcgt
     1801 ggacgtcgaa ggagaccggc gagacggcgc ggacctcgcg agggccctga ccgaaggtct
     1861 tcgcgaggtc ccggatggag acgcggacgc gcggcgtcac tcccggcggc ttacttgtac
     1921 ttggcctgcg ccttcttgac gaagctcatg tccaccgcct gcttgaacgg aatcggcttc
     1981 tccaccgcgg tgggcaccca caccttcatg ccgcgctcgt agtctcgctc ctcgatcacg
     2041 aagtcccagt cgaagatcgt ccggtagtag cggacggact cgagcaccac ctcgcgcttg
     2101 aaggtgtcca tgtacggctt ccagagcagg tcggtgatct cgtcgtcctt ggcgcggcgg
     2161 atccactgct gcgcgcggta gcaggcgttg acgtaggcct gcacgaggtc gggcgacttc
     2221 tcgatggttt ccttcaggac gtagcccacc gtgaccggga tgggtccgcc gaagacgcgg
     2281 ttccacgcct tctcgtcctg gatgtcgtag agcggcttgc cgaagccttc ctccaccgcc
     2341 gccgattgcc actccggcac cgccatgatc gcgtcgaact tgccggcctt gaggccgccg
     2401 agcatcgtgg tcgaggcacc cccgcccacc cactcgacgt ggtcgttcac cggcttgccg
     2461 tccccgccct tgaggttctg cagcacgtac accccgtaga cgtaggtgcc ggagccgatc
     2521 gcggtggccg cgaccaccgc cttgcggccc accagcttct ggtcggcgag cgcctcgacg
     2581 gtcttcacgc ccttgtccca cagctccttg cgtacgacga tgttggcgta ggaacagcgc
     2641 gtgtcggtgg cgaagaccat cagcgcgtcc ttgcccttct cggtgatctt gagcgggtgg
     2701 ttggagtcgc cgagcgcgaa caggacctgg cccgcggcca gcgcctgcac gaccttggcc
     2761 ccgcccccgg ggaccacgaa gtcgggcgtc ttgacacctt cctcctcgaa gagcttcttc
     2821 tgcgcggcca ccaggtgctg gccgtagacg aaggtcgaga cgctatgcga ggccgagatg
     2881 ctcttcaggc cctgggccca ggcccgcccc gatcgggacc ccccgaccgc cagaccgccc
     2941 gccaccgcgg tggatttcaa gaactcccgg cgcgtccagc gcgccggctg cggctgctcc
     3001 gccatggtcc cctccttggg aaaagtccga gaaaactgcc aggcactcta catgccgccg
     3061 gcccgggctg tcaacgcttg attggcccaa agacattgac acataaggtt ttttgggcta
     3121 ttgtccggtg atggtagcgc gggagttccc cctcaccacc acctccgagc cttcgagcga
     3181 ccgatgatcc gcagcatgac cggctatgga cgggcggagg cggcgggggc gcgcaccatc
     3241 ctgtcggtcg agtgcaagtc ggtgaaccac cggcacctcg acgtctccct caagctgccc
     3301 cgggtcctgg gcgccttcga agcggacgcg cgacgactga tccaggccgc ggtgcagcgc
     3361 ggccgcgtgg acgtgagcgc gacggtgacc gcgcccgaag gcaccgtgct gaatccgctc
     3421 accgtcaacc tggcccaggc caaggagtac gccgaggccg cccgcgcgct cgcccaggct
     3481 ctcgatctcg gagacgcccc gtcgctcggc tgggtgatgg gccagtcggg cgtgctgacg
     3541 cgcgaggagc aggcacccct gtccgccgag gaggcgtggc ccctgctcga gcggtcgctc
     3601 agcgccgccc tggcggagct ggtggagcgg cgcgagaccg agggccgcgc gctcggccag
     3661 gagctggcgg gcctcggcgc gaccctggcc gcccacgtgg acacggtggc ccagcgcgcc
     3721 ccgatggccg tcgagcggcg ggccgtccgc ctccgcgagc ggatgcaggc gatgctggcg
     3781 ggcgcggaga tcgaccaatc gcgcctggcc accgaggtcg cggtgtgggc ggacaagacc
     3841 gacgtgagcg aggaggtggc ccgcctgcgg gcccacctga cccagctggg cgccctgctg
     3901 gccgcggacg gcccggtggg ccgcacgctc gatttcctca tccaggagat caaccgcgag
     3961 gtgaacacca tcggctccaa ggcggacgat ctggagatct cccaggcggt gatcgcggcc
     4021 aaatccacgc tcgagaagat gcgcgagcag gtggccaatc ttgagtagcc cgggccgctg
     4081 ggcgagctgc cgccgcaggc gaggcgagcg gatatgaaaa ccccgtgcag ctgatcaagc
     4141 gccgcggagc gctggtggtg gtgtcggccc cgtcgggggc gggcaagacc acgctctgcc
     4201 acgaggtacg ctcgctcgtg cccgagctct actactccgt ctcctacacc acgcgcgatc
     4261 cgcgccccgg ggagaaggag ggcaccgact tcttcttcgt ctccgagggc cggttcaccg
     4321 ccatgcgcgg ccgcgacgag ttcgcggagt gggcccaggt gcacggccac cactacggca
     4381 cgccggccaa ggccctcgag ggcgcgctga gccggggcct cgacgtgctg ctcgatatcg
     4441 acacccacgg cgcccggcag ctccgccagc gctatccgga ggcggtatcg gtcttcatca
     4501 tggcgccctc gatggccgag ctcgaggccc ggctgcgcga gcgcaagagc gattccgccg
     4561 gcgacatcgc gcgccggctg agccgcgccc gagaagagat cgcggcgtgg cgccagtacg
     4621 actacctcat catcaaccgt gacgtgaagg acgcggtgga ccagctcgcc acgatcatcc
     4681 aggccgagcg ctgccgcacg agccgcctga ccctcagatt ccccgacatg gaggtacccg
     4741 cttgatgtcg ttcccatcgc tggaaaagtc cctggacaac gtctccaacc gttacatgct
     4801 ggtggtgctc gcggccaagc gcgcgcgcca gctcaaccgc ggcgccaagt cccaggtcga
     4861 gagccgccac aagaagccca ccagcaacgc cctggaagag atcgccgaat cccgggtggg
     4921 atacagagtg aaggaagaag acgcaccgaa ggcctgaagc gagaccaaag caaaacacgt
     4981 aggcctgaaa cgagtgtccg cggactgggg gagtgcgggg gccatttctg ggcccccgca
     5041 ttcgatagag tttagagact tagaccgtaa gccgggcgta cggtaaccgg gcgcaaccat
     5101 gaccctccac gacaaagagc tgatcctcgg cgtcaccggc agcatcgccg cctacaaggc
     5161 ggtgttcctg ctgcgcgagc tcacgcgcgc gggcgcgcag gtcacggtgg tcaccaccgc
     5221 gcacgccgag cgcttcgtcg gcgccctcac cttccgcacc ctgtccggcc ggccggtgct
     5281 ctccgatctc ttcgatcccc ggagcgcgga ggcggtcgag cacgtcgccc tggccgagcg
     5341 ggcgcaggcc atgatcgtgg cccccgccac cgccaatctc ctggccaagg ccgcccacgg
     5401 catcgccgac gatttcctca ccacgctcct gctcgcggcc cggtgcccgg tgctgatggc
     5461 gcccgcgatg gacatcggca tgtgggatca cgcggcggtg accgcgaacg tgctgacgct
     5521 gcgcgggcgc ggggtcacgg tgctggagcc cgacgcgggc gcgctcgcct ccggcctctc
     5581 gggccgtggc cggctgcccg aggccgacgc catcctcgag gcgctcgagc gcctcctggc
     5641 cccacggcgc gacctggtgg gcgagcgcgt gctggtgacg tcggggccaa cccgcgagcc
     5701 catcgatccg gtgcgctaca tctcgaatcg ctcgtccggg aagatgggcc aggcggtcgc
     5761 gaccgccgcg ctgcgccgcg gcgccgaggt ggtcctgatc tccggcccca ccacgctgac
     5821 cccgcccgcg ggcgcggtgt acgtgccggt gcagaccgcg gaggacatgc gcgaggccgc
     5881 gctccagcat ctcgagcggg ccaccatcgt catcaaggcg gcggcggtgg ccgactaccg
     5941 cgtcaagcag ccgagcgcgg ccaagatcaa gtcccgcaag gacgaggggc tcacgctcga
     6001 tctcgccccc aatcccgaca tcctgaagga gctggccgcc cggaagggcg gtgcgttcct
     6061 ggtgggcttc gcggccgaga ccaacgaagt gcgggccaac gcggccgcca agctccgggc
     6121 caagggcgtg gatctcctcg tggccaacga cgtcagcgtg aagggcatcg gcttcgacgc
     6181 cgaggacaac caggtcttgc tgctcgaccg ctggggcggc gccatggatc tgcccaagat
     6241 gagcaagctg gacgtcgccg acgccattct cgatcgcgtg gtcgggctgc gcgcggccga
     6301 tcgctccccc gcctccaacc gcgccacccg ctaggccgat gagcgacccg cgggaggccg
     6361 tggccctggc cctgcgcgga ctggcggaca ccctccggca ccaccgcgat ctcggcctgc
     6421 gcgacctcgc gctctcgacc gtgccgttcc ccgatccggc cgacgccctg cgtgagcagg
     6481 agcgggcgcg ccagggctgc cagctctgca agctctgcaa gagccgcacg aacgtcgcgt
     6541 tcggctcggg caatgcgcgg gcgcggctca tggtggtggg cgagggcccc ggcgaggaag
     6601 aagacaagca ggggctgccc ttcgtcgggc gcgccggcca gctgctcacc aagatgctgg
     6661 cctcggtcgg gttcgaccgc gagcgcgatt gctacatcgc caacgtggtg aaatgccggc
     6721 ccgagcgcaa tcgcaacccg gagcccgacg aggtcgcggc ctgcagcccg ttcctgatgg
     6781 cgcagatcga caccatcggc ccgcacgtga tcctggccct gggcaacttc gccgcgcaga
     6841 ccctgctcgg gaccaaggaa ggcatcaccc ggctgcgagg gaagacgtat cagtaccggg
     6901 cgagcgtgct cgtgcccacc ttccatcccg ccttcctgct gcgcaacccc ggccccgaat
     6961 tcaggcgcat ggcctgggac gatctgaagc tggcgcggcg ggagtatgac gcccgctccg
     7021 gcgatggccc gcggtaggtc cacgaatgtc cgcggactgg gggagtgcgg gggccatttc
     7081 tgggcccgag cacccgccca cgctttgatc gccgacgttg tcttcgatat tcccatcgat
     7141 cacgcgttct cctacgtcgt cccgcctgag ctcgtggtcg ctcgggggca gcgggtcggc
     7201 gcgccgctcc acggccgggc gcgggtcggc gtggtggtcg ggctccgcga gggcgatccg
     7261 gccgggctga agccgctgca gcgcgcggtg gagccggtgc ccgtgctctc ggaggccgcg
     7321 ctcgagctgg gacgctgggc cgccgccgag agcctgtcgt cgtggggctc cactctcctc
     7381 tcgctgctgc cgccgccccc acgtgggggc gcggccgagg cggtgtcgcc gccggctgcg
     7441 cccgcgcccg gcccgccccg cgccccggaa ctctggacgg acgagcgccg cgaggaccgg
     7501 ctcgtggaat gcctggagcg cgagcccggc gccacgctcc tcatcgcgcc ggaccgcgag
     7561 gcggccgcgc gctgggcacg gcggctcgac gccccccggc tcgacagcgg cgtcacggag
     7621 gcggagcgcc gccgggcctg gtttgcggcc tcccgcgggc gcagccgcgt cgtggtcggc
     7681 acgcgctccg cgctgctcgc gccactgccg ccgcccgcga cgctggccct gatcgacgag
     7741 cacgatccgg cccacaagcc gccgggcgcc ccgcggctgc attcgcgcga cctgctactg
     7801 cggcgggccg agctcgacgg cagccgcctg ctcctgctct ccgccacgcc gtcggcggag
     7861 agctggtggc acgcacggca gggccggatc gtccggccgg acgccccggc cgcgccctgg
     7921 ccccaggtca tcaccgccga cacccgcggc atcctccgga accatccgct caccctgccg
     7981 ttgacgcgcg ccatcgagga catgtcgcgg caggggcgcc gcgccgtcct catcgtcagc
     8041 cggcggacgg ccgcgctggc ctgcgacgag tgcggcgccg tcctgcgctg ccccgactgc
     8101 ggcgtggccc tggcgctcgc ccgagaccgg agcgcgctgc gctgtccgct ctgcgcgcgc
     8161 gccgagacac cacccgatca ctgtccggcg tgcggcggcc accggctctc tccgttcggc
     8221 tgggatcccg agcgagtcca gacctccatc gcgcgccggt tcccccggct gacgatctcg
     8281 cgccagagtc tcgaggccca ggtgctcatc ggcacgcccg cgctgctccg ggcgctgccc
     8341 cggcgctccg tgggctgcgt cggattcgtc gccctcgacg ggctgctgcg cgtgccggat
     8401 ttccgcgcgg gcgagcggac ctggcagctc ctgtgggccg ccgcggaatc ggtggtgccg
     8461 ggcggccgcc tcgtcatcca gacacagcac cccgagcatt atgcgatccg ggccgtgcag
     8521 gcgcaggatc gcgcgagctt ctacgacgag gagctcaagt tccgggcgga gctgggctat
     8581 ccgccctacc gccggctcgc cgaggtttcg gcccggggcc gggaggacgg cccggtgcga
     8641 gccctcgccg cccagtgcgc ggcggccctt cgcggcatcg gcggcctcac cgtctatccg
     8701 cccgcctcgg tgactccccc gcgcgggcgc aatagtcggt ggcgcttcgt gatcaagggg
     8761 cccgccgagc tgccgcggct cctggccccg gcgctcgagc ccttcctcgg gcagcgacgg
     8821 cgctcgagcg atatggtaga aatagagatg gatccgcaat ccaacggctg aagatagggc
     8881 ccagagaggg gacgagacag tggcgttgct cacggtgagg ctctacggcg acccggccct
     8941 gcgtcaggta gccgccccgg tgcgcgacat caccgccgag atcaagcgga tcatcgccga
     9001 catgaccgag accatgtggc accaggtcgg catcgggctg gccgcgcccc aggtcgggct
     9061 gccgtatcgc attctcgtga tggacgacgg caagggcggc gcgcagaccc tgatcaatcc
     9121 ggagatcgag acccgtagcg ggacggtccg cgaggaagag ggctgcctct cgctcccggg
     9181 catcttcggc gtggtcgagc gcagcaagac gatcaccgtc cgggcaatgg acgcggacgg
     9241 caagcccgtc tcgctcgagg ccaccgggct caaggcccgg atcatccagc acgaactgga
     9301 tcacctcgac ggcgtcctct tcatcgaccg gctgcccccg gtgacccggg accgcatcaa
     9361 gaagaagatc cagaaggaag gcttccccaa ggagacgggg aaccacgcct tcgcgctcta
     9421 gcgggtcccg cctgcgcgtc ctgttctacg gcacgccggc cttcgccctg cctactctcg
     9481 aggctctgct ggcgcgccac caggtggtgg cggtgatcac ccagcccgac aagccggcgg
     9541 gccgcggccg tgccgtgacg gcctcgccgg tgaagcggcg cgctctcgtg gcctcggttc
     9601 cggtgctgca gccgccccgg ctccgcgatg cgggctggcc cgagcgcctg gccgagtacc
     9661 gggccgacgt ggcggtggtt gccgccttcg gccagatcct cccgaaggcg gtgctcgacg
     9721 tgccggttca cggctccatc aacgtccacg gctcggtgct gccgcgctat cgcggcgcgg
     9781 cgccgatcgc ctgggccatc atgcgcggcg agacggagac cggcatcacc accttccaga
     9841 tggacccagg aatggacacc ggggacatgc tcctgaccga ggcaacgccg atcggccccg
     9901 aggagacggc gggcgagctc tccgtgcggc tggcggagat cggggcgcgg ctcctgctgc
     9961 ggacgctcga tggcatcgac acgctgccgc gcatcccgca agaccacgcg caggcgacgc
    10021 tcgccccacg gctcaagaag gaagacggtt ggctgcggcc ggccgagccc gcgcgtgatc
    10081 tcgtcaaccg tgtgcgcggc tgcaacccat ggccgggcgc cgcgctcatc acgccggccg
    10141 gccggctcct gatctggcgc gccaccgcgg tgcccgcgcc cggcgtcacc gcgccctccg
    10201 gcagtcttgt cgcctctgag ggggccctcg ccatcgccac cggcgacggc ctgttcctcc
    10261 cgctcgaagt gcagccggag aatcgcaagg cgatgcgatg ggccgacttc ctccgcggcg
    10321 cgcgcctggc agccggcgcg cgcgtcagcg agcccacccc gtgagcccga tcctcaaggc
    10381 gggcccggtc tcccaggcgc gttacgaggc gctccacatc ctggttcgcg tcgagcggga
    10441 ccgcgccttc gccgatctcg cgctcgaggc cacgctcgat cgggcgcatc tcg
//
LOCUS       DASUFA010000072        12365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_351809, whole genome shotgun sequence.
ACCESSION   DASUFA010000072 DASUFA010000000
VERSION     DASUFA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351809"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..1025)
                     /locus_tag="VFX87_02795"
     CDS             complement(447..1025)
                     /locus_tag="VFX87_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX4993862.1"
                     /translation="MIPALNLNRVVVVGTSCAGKTTLARRLADILASEHVELDSIYWG
                     PAWTPRPDFIQTVLAIAQQPRWVIDGNYSAVRDIIWRRSSAIVWLDYSFARVFSQALR
                     RTARRILTRERLYAGNRETVRNALLARDGIPWWVIRTHGKRRRDFPELLRRPDYRHAA
                     VIQLDRPAAAEAFLLAVSAGPRTTNSGHTHAD"
     gene            complement(1224..2213)
                     /locus_tag="VFX87_02800"
     CDS             complement(1224..2213)
                     /locus_tag="VFX87_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEX4993863.1"
                     /translation="MPDPTPSPPPRLLDRVRAALRTRHCSPRTEKAYVGWTRRHILFH
                     GKRHPADMGAAEITRFLSALAVQRNVAASTQNQALSALLFLYREVLEQELPWLDDIVR
                     AKTRERIPVVLTRDEVQAAVAGLDGPPRLLALLLYGAGLRLLEALRLRVKDVDFARNQ
                     ITVRSGKGGKDRVTMLPAAVRRDLAKHLEVVRAQHEADVRAGAGWVELPWAFARKYPR
                     AAREWIWQWIFPATRPYLHRDTGQRRRHHLHETVLQRAVKEAVRSAGIPKGATCHTFR
                     HSFATHLLEDGADIRTVQELLGHRDVTTTMIYTHVLNRGPAGVLSPADRLPVL"
     gene            2292..3263
                     /locus_tag="VFX87_02805"
     CDS             2292..3263
                     /locus_tag="VFX87_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4993864.1"
                     /translation="MPFAYFRRLTRRQQAIYLESDGIVTMRLPEAPRLQPLVTVLARA
                     LESGDRATTESAAQRLVGGLARALGAPPVRVKVLAARPHAGWGELHGLYESPGRGEPP
                     LITLWMRTARQKRVVAFRTFLRTLLHEVGHHVDYTLLRLGDSFHTQGFYARESHLFHQ
                     LVTDGGVLMASVEEQMARMERTAEDFAAAIKGVSEAALSRRPDDKSWSAKETLCHVRD
                     TEESFMLRFQTIMEMDEPRFLPGDPDRWATERQYQRNDAGEALQAFRVRRDETLKFLR
                     GLRTEHWDRGGVHPTRGRMTVTDFVGLMAWHDDNHLDQLKRALAGKP"
     gene            complement(3321..3461)
                     /gene="ccoS"
                     /locus_tag="VFX87_02810"
     CDS             complement(3321..3461)
                     /gene="ccoS"
                     /locus_tag="VFX87_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX4993865.1"
                     /translation="MSVMFVLLPVALLFAAVAVGVFVWAARAGQFDDLETPSIRILHD
                     DD"
     gene            complement(3458..5920)
                     /locus_tag="VFX87_02815"
     CDS             complement(3458..5920)
                     /locus_tag="VFX87_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX4993866.1"
                     /translation="MATTESVAPARAVRAPCAHCGLPVPPGADGDEAGRRFCCEGCRA
                     VWAILHEHGLERYYALREGAAPAPAAPVDRLYAELDDAGFQARTCRPEPGGRVSTELY
                     LEGVHCSACVWLVEKLPGLVPGVAETRLDLPRSQVRVVWDPRVVPLSAIARRLGSLGY
                     TPHPCRGLAGQAARRREDRTMLARIGLAGAVAGNVMAIAFALYGGMLDGMEPEFAAFF
                     RWASLLLTMPSLIWGGGVFFRSAATALRAGTLGMDLPISIGLLAGFLHGVVNTVRGTG
                     EVYFDSVTVLIFLLLSGRYLQRRQQRAAGDATELAASLYPTSARLIDDGRVREVPLET
                     LAPGLVVEVRAGDLVPADGVILAGRSTVDLSLLSGESAPQSVAEGATVHAGTLNVSSR
                     IEVRVERTGEDTRVGRLLALVEEHARRRAPIAQLADRIAGHFVGVVLTLAAATFVLWA
                     RVDPSRALDHAAALLIVTCPCALGLATPLAVSAAIGRAARAGFLIKGGDVLEKLTRPG
                     RMWLDKTGTLTEGRAALLRWHGDPAVKPLVAAAEAHSSHPLGRAFLRALGEPAPGGRV
                     EIEETHGGGIEATVDGRRLVVGSADFVAARAAAVPPALAAAVAELAGDGLTPVLIAVD
                     GRVTAAAGFGDPLRADSAAALERVRALGWRPGILSGDDARIARAAGRGLGLAAPDCRG
                     GVAPEDKLRVVLGSAREGPVVMVGDGVNDAAALAAATVGIAVAGGAEAALTAADVFVV
                     RPGAARIAELLEGSRRAMRVVRRNLAFSLGYNVIGVTLAMTGLLNPLVAAVLMPLSSL
                     TVIISSYRARTFARPAQEAGRP"
     gene            complement(5907..6629)
                     /locus_tag="VFX87_02820"
     CDS             complement(5907..6629)
                     /locus_tag="VFX87_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4993867.1"
                     /translation="MSVLIATVLGASLLGSAHCAGMCGGFVGFYTGTTPGARRRALLL
                     AHLAYNGGRLGVYAALGAAAGAAGAALDTTGGVLLGIQRTAAIVSGALITLWGARSLL
                     EAWGVRVPRLGAPAGLRTLAGRGIGAVAAAPPPLRALVVGLATGLLPCGWLYVFLATA
                     AGTGTALGGAALMAVFWVGTLPAMLGLGLGLQALAGPLRRHVPVVCAIAMIVVGLLAI
                     AGRINSVDPGAGATTQQRHGHH"
     gene            complement(6626..7111)
                     /locus_tag="VFX87_02825"
     CDS             complement(6626..7111)
                     /locus_tag="VFX87_02825"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX4993868.1"
                     /translation="MAARGWYWPVIVVALLVGGAGANIGFMIVANRDASFAVEPDYYR
                     KALEWDQVMAQEARNAELGWTVSARLERAGAGPARLVAAVRDRAGAALGGATVTVEAF
                     PSARAREIVSLTLEPTGDAGVYAAALPAGHPGLWELRVRVSRSGEVFTRTLSQDLAAS
                     R"
     gene            complement(7101..8570)
                     /gene="ccoG"
                     /locus_tag="VFX87_02830"
     CDS             complement(7101..8570)
                     /gene="ccoG"
                     /locus_tag="VFX87_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX4993869.1"
                     /translation="MSFPLEAEGRVLPTLNPDGTRRWLRPRLFPGRFFKRRRAVAWVL
                     LALFTVIPYLSMNKRPLILLDVVHREFTLFGATFLPTDSVLLMLLLVGLFLAIFLITA
                     VHGRVWCGWACPQTVYMEFLYRPIERLIEGGTREQERLDASRFRPRRLLKHAVFLALS
                     MFLAHTFLAYFVGVSELARWVQRSPFEHPIAFLVMAATTALMLLDFGIMREQVCMVAC
                     PYGRLQSVLLDRRSLIVGYDRRRGEPRSKLVRSGLTLVGKAPHAGDCIDCGACVACCP
                     TGIDIRDGLQMECIHCTQCIDACDSIMDRIGRPRRLIRYSSREELAGEPRRLLRPRVV
                     VYPLLLLLVWGTLAYALAHRAPADVTVLRGLGSPFTVLPSGLVSNQIRVKIVNRDSVE
                     HRYLVELVEGAADPKARLELIAPENPLPVAAGKSATAPIFVTASHKAFEDGRRPVSLR
                     ISDGTGWSTVVPYRLLGPEEDDHDKDKDHDRDKGKDHGR"
     gene            complement(8579..9184)
                     /locus_tag="VFX87_02835"
     CDS             complement(8579..9184)
                     /locus_tag="VFX87_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4993870.1"
                     /translation="MSPTDRDRLLAHDYDGIQEYDNPLPSWWNWLFLATIVFSVGYYL
                     YYQIGPGPSLIAQYEREMGELAQQQARLAPSAGAAGAGEDALRALMKDAKAMAAGKEM
                     FAVRCMPCHGPEGQGIIGPNLTDDYWLHGRALADIRRTIHDGIPDKGMIPWKDQLKPA
                     EIDTMTAYVATLSGTKPPNPKPPQGVNSKGEVAPEAPPASQ"
     gene            complement(9181..9378)
                     /locus_tag="VFX87_02840"
     CDS             complement(9181..9378)
                     /locus_tag="VFX87_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993871.1"
                     /translation="MKLGDVMSAMHFNLFAQIALIVAVAAFAVVVVSTFLARNREPFR
                     RASLMPLADDAAPTGTRGGRA"
     gene            complement(9375..11579)
                     /gene="ccoN"
                     /locus_tag="VFX87_02845"
     CDS             complement(9375..11579)
                     /gene="ccoN"
                     /locus_tag="VFX87_02845"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX4993872.1"
                     /translation="MAGSAVPGQRMDRFVYDDAIVRLFLLATLVWGLVGMLVGVVIAL
                     QLTNPIFNFKTSWLSFGRLRPLHTNAVIFAFAGNAIFTGVYYSAQRLLKARMWSDGLS
                     RLHFWGWQAIIVAAALTLPFGITQSKEYAELEWPVDVAIALVWVVFAVNLFGTIVRRR
                     ERHLYVALWFYIATVVAIAVLHIFNNLVVVAGPFKSYSIYAGVQDAFMQWWYGHNAVA
                     FFLTTPFLGMMYYFMPKAADRPVFSYRLSILHFWTLVFIYIWAGPHHLHYTALPAWAS
                     TLGMLFSVMLWMPSWGGMVNGLLTLRGAWHKVTEDPVLKFFVVAVTFYGMSTFEGPLL
                     SIKSVNALSHYTDWTIAHVHAGALGWVGFMVFGMVYWLLPRLFQAELWSRRLAELHFW
                     IATVGILLYIVAIYAAGLTQGLMWRAFDETGRLAYPDFVETTVRLIPMYWVRVLGGSL
                     YIAGLILCGVNTLMTWRGRPARYAEPVHEAPALAPAYTPEAAPGGFRRMGWHRRWEGL
                     PLTFSVWVAVAVVTASLFEIIPMFLIGSNVPTIQAVHPYTPLELAGRDLYVAEGCNNC
                     HSQMIRPIRAETMRYGEYSKPGEFVYDHPFLWGSRRIGPDLARVGGKYPDLWHVRHME
                     DPRSTTPQSIMPAYPWLARARINWAALERGVAAQATLGVPYTRDEVAGAATLGRKQAQ
                     QVAAAIVQQGGPAGLEDTQLVALIAYLQRLGTDIKRSPKVAAAPAGGEGTGR"
     gene            complement(11860..>12365)
                     /locus_tag="VFX87_02850"
     CDS             complement(11860..>12365)
                     /locus_tag="VFX87_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4993873.1"
                     /translation="DAHGRTPLHVAAFGAHHDAMRALAAAGADPNALERDRYDIVTIA
                     AVANDMPTLKLALELGASARNVTSRYDGTALIAAAHLGHAEVVRTLIRAGAPLDHVNN
                     LGWTALIESIVLGDGGRRHTETLAALIEAGANVNLADRSGRTPLALAEARGHAAMVER
                     LRRAGAR"
BASE COUNT         1913 a         4329 c         4535 g         1588 t
ORIGIN      
        1 agccacgcca acctcacgaa gcactacagc acgccgacca aatggccggc gtagaaagcc
       61 tgccccaagg cgcccacaat gctcaaaccg acgagtgaaa ggttcggcca tgtccgattc
      121 tctgactgac ggagcgctct ccatcccagg atgacgccgg tgagaagagc gaaaagcatc
      181 gcgacctggg caagcataaa accgaggact acggccattg cttcgccaag gccaggttcc
      241 cttggctcac cggccacata cggtatcgac gcgatcaaca gccagaggag cgcgtatccg
      301 ctccaaggcg aaagaagaat tcgacgcgct agagtcactg ggtcaaccct ttcgtcatcg
      361 cagcctaacg tatggctcag cggcgcgcgg agcgcgtccg ctgaagccgg aagttggaca
      421 gccctatcgc acggcgtcct cttgcttcaa tctgcatgag tatggcctga gttggtggtt
      481 cttggaccgg cgctcaccgc caagagaaat gcttcggctg ccgctggtct gtcgagttgg
      541 atcacggccg catgacggta gtcagggcgc ctgaggagtt caggaaagtc gcggcgccgc
      601 ttgccatgcg tccggatcac ccaccagggg attccgtcac gggccagcaa cgcgtttcgg
      661 accgtctcgc ggttgccggc gtacaggcgc tcacgcgtga gaatccgccg ggccgtgcgt
      721 cgaagagctt gtgagaacac gcgtgcgaaa gagtagtcga gccacacgat ggcggacgac
      781 cgccgccaga tgatgtctcg caccgccgag tagttgccgt cgatgaccca gcgaggctgc
      841 tgagctatgg ccagcacagt ttggatgaag tccggccgcg gtgtccacgc ggggccccaa
      901 tagattgaat caagctcgac gtgctcgctg gcaagaatgt ctgccagccg ccgtgccagc
      961 gtggtcttcc cagcacaact ggttccgacc acgaccacac ggttgaggtt cagcgccgga
     1021 atcatcctct gtctgcccaa ctagaagtat ccagttctag ataacgcggc cgcattgcgg
     1081 gttggtgagg atattcccgc cggggtggcc ggggcggtgt ggctggagcg gccttcgggg
     1141 gcgcgtggtt gcgcatggtc ccggggtgcg gcggtagtgt tacacagacc ggggtatttc
     1201 gggagggggt ggcggcggct gggtcagagg accggaaggc ggtcggcggg gctcaggacg
     1261 ccggcggggc cgcggttgag gacgtgggtg tagatcatgg tggtggtcac gtcgcggtgg
     1321 cccagcagct cctggacggt gcggatgtcg gcgccgtcct ccaggaggtg agtggcgaag
     1381 gagtggcgga aggtgtggca ggtggcgccc ttggggatgc ccgcactgcg taccgcctcc
     1441 ttcaccgccc gctgcagcac ggtttcgtgc aggtggtggc ggcggcgctg gccggtgtcg
     1501 cggtgcaggt acggccgggt ggcggggaag atccactgcc agatccactc gcgggcggcg
     1561 cgcgggtact tgcgggcgaa ggcccagggc agctccaccc agcccgcgcc ggcccggacg
     1621 tcggcctcgt gctgggcgcg gaccacctcg agatgcttgg cgaggtcgcg gcgcaccgcg
     1681 gcgggcagca tcgtcacgcg gtccttgccg cccttgccgg agcgcaccgt gatctggttg
     1741 cgtgcgaagt ccacgtcctt cacccgcagg cgcagggctt cgagaagccg caggcccgcg
     1801 ccgtagagga gcagcgccag gaggcggggc gggccgtcga gcccggcgac ggcggcctgc
     1861 acctcgtccc gcgtgagcac caccgggatc cgctcgcgag tcttggcccg gacgatgtcg
     1921 tcgagccacg gcagctcctg ctccagcacc tcgcggtaga ggaagagcag cgcgctcagc
     1981 gcctggttct gggtggacgc ggccacgttg cgctggacgg ccagggcgct caggaagcgc
     2041 gtgatctcgg ccgcgcccat gtccgccggg tggcgcttgc cgtggaagag gatgtggcgc
     2101 cgggtccagc cgacataggc cttctcggtg cggggactgc agtgacgggt gcgcagcgcc
     2161 gcccgcaccc ggtcgagcag ccgcggcggg ggtgaaggcg tcggatccgg catgcgcgag
     2221 gctaggcagg cctggcgccc gagcgcaaga cgtggacacg cggatacccc ggcggtagca
     2281 tccagagccg gatgcccttc gcctatttcc ggcggctgac gcggcggcag caggcgatct
     2341 atctcgagag cgacggcatc gtcaccatgc gcctgcccga agccccgcgg ctgcagccgt
     2401 tggtaaccgt gctggcccgc gcgctcgaga gcggcgatcg cgccactacc gagtcggccg
     2461 cgcagcgcct ggtcggcggc ctcgcgcggg ccctgggggc cccgccggtg cgcgtgaagg
     2521 tgctggcggc acgcccgcac gcggggtggg gcgagctgca cgggctgtac gagtcacccg
     2581 ggcgcggcga gccgccgctg atcacgctct ggatgcgcac cgcgcgccag aagcgtgtgg
     2641 tggccttccg caccttcctc cgcacgctgc tgcacgaggt gggccaccac gtggactata
     2701 ctctgctgcg gctgggcgac tccttccata cccagggctt ctacgcgcgg gagtcgcacc
     2761 tgttccacca actggtcacc gacggggggg tcctcatggc gagtgtcgag gagcagatgg
     2821 cgcgcatgga gcgcacggcg gaggatttcg cggccgccat caagggcgtg tccgaggccg
     2881 cgctcagccg gcgcccggac gacaagagct ggtcggccaa ggagacgctc tgccacgtgc
     2941 gggacaccga ggaatccttc atgctccgct tccagaccat catggagatg gacgagccgc
     3001 ggttcctgcc cggcgatccc gaccgctggg ccacggagcg ccaatatcaa agaaatgacg
     3061 cgggggaagc gctgcaggcc ttccgggtgc ggcgcgacga gaccttgaag ttcctgcgcg
     3121 ggctccggac cgagcactgg gatcgcggcg gcgtgcaccc cacccgcggc cgcatgaccg
     3181 tcacggactt cgtgggcctc atggcctggc acgacgacaa ccacctcgat cagctcaagc
     3241 gcgcgctcgc gggcaagccc tagccccctc accctgccct ctcccccgca agggggagag
     3301 gggaacccaa ccgggccggc tcagtcgtcg tcgtgcagga tccggatgct cggggtctcg
     3361 aggtcgtcga actgcccggc tcgcgcggcc cagacgaaga cgccgacggc caccgccgcg
     3421 aagagcagcg ccaccggcag cagcacgaac atcacgctca tgggcgcccc gcctcttggg
     3481 cgggccgggc aaatgtccgg gcccgatagg agctgatgat cacggtcagc gacgacagcg
     3541 gcatgaggac ggcggccacc agcgggttga gcaggccggt catggccagg gtcaccccga
     3601 tcacattgta gccaagcgag aaggccaggt tccgccgcac gacccgcatc gcccgtcgtg
     3661 acccctccag cagctcggcg atccgggccg cgcccggccg gaccacgaac acgtcggcgg
     3721 ccgtgagcgc ggcctccgcg cccccggcca ccgcaatgcc caccgtggcg gccgcaagcg
     3781 cggcggcgtc gttgacgccg tcgccgacca tgaccaccgg cccctcgcgg gcgctcccga
     3841 gcacgacgcg gagcttgtcc tcgggcgcca ccccgccccg acagtccggg gcggcgaggc
     3901 ccaggccgcg gccggccgcc cgcgcgatgc gggcgtcgtc gccggagagg atgccgggcc
     3961 gccacccgag cgcgcgaacg cgctcgaggg ctgccgccga gtcggcgcgc agcgggtcgc
     4021 cgaagccggc ggcggccgtc acccggccgt ccaccgcgat cagcacgggg gtgagcccgt
     4081 cgcccgccag ctccgccacc gccgcggcga gggccggcgg cacggcggcg gcgcgggccg
     4141 cgacgaagtc cgccgagccg acgacgaggc gccgtccgtc cacggtggcc tcgatgccgc
     4201 cgccgtgcgt ctcctcgatc tcgacgcgcc cgcccggcgc gggctcgccg agggcgcgca
     4261 ggaacgcgcg cccgagcggg tgggaagagt gcgcttcggc ggcagccacc aggggcttga
     4321 ccgccggatc gccgtgccag cgcagcaggg ccgcgcgccc ctcggtcagc gtgccggtct
     4381 tgtcgagcca catgcgcccc ggccgggtca gcttctccag cacgtcgccg cccttgatga
     4441 gaaagccggc gcgggcggcc cggccgatgg ccgcgctgac cgccagcggc gtggccagcc
     4501 cgagggcgca ggggcaggtg acgatgagca gggccgcggc gtggtcgagg gcgcgcgagg
     4561 gatcgacgcg ggcccacagc acgaacgtgg cggcggccag cgtgagcacg acgcccacga
     4621 agtggcccgc gatccggtcg gcgagctggg cgatcggcgc gcggcgccgc gcgtgctcct
     4681 ccaccagcgc gagcagccgg cccacccgcg tatcctcgcc ggtgcgctcc acccggacct
     4741 cgatccggga cgacacgttg agcgtgccgg cgtggacagt ggcgccctcc gccacgctct
     4801 ggggcgccga ctcccccgag agcagggaga ggtccacggt ggagcggccg gcgaggatca
     4861 cgccgtcggc gggcacgaga tcgcccgcgc gcacctcgac cacgagcccg ggcgcgagcg
     4921 tctcgagcgg cacctcgcgc acccggccgt cgtcgatcag ccgcgccgaa gtggggtaga
     4981 gcgaggcggc cagctcggtg gcgtcgcccg cggcgcgctg ctgccgccgc tggagatagc
     5041 gcccggacag caagaggaag atcagcacgg tcacggagtc gaagtagacc tcgcccgtcc
     5101 cgcgcacggt gttgaccacg ccgtggagga agccggccag gagcccgatg ctgatcggca
     5161 ggtccatgcc gagcgtgccg gcgcggagcg cggtggccgc gctgcggaag aagacgccgc
     5221 cgccccagat cagcgacggc atcgtcagca gcaggctcgc ccaccggaag aaggccgcga
     5281 actccggctc catgccgtcg agcatcccgc cgtacaaggc gaaggcgatc gccatcacgt
     5341 tgcccgccac cgcgccggcg aggccgatgc gggcgagcat ggtccggtcc tcgcggcgcc
     5401 gcgcggcctg cccggccagg ccgcggcagg ggtgcggcgt gtagccgagc gagccgagcc
     5461 gccgcgcgat cgccgagagc ggcaccacgc gcggatccca gaccacgcgc acctgcgagc
     5521 gcggcaggtc gagccgcgtc tccgcgacgc ccgggacgag gcccggcagc ttctcgacga
     5581 gccagacgca ggcgctgcag tgcacgccct cgaggtagag ctcggtcgag acgcgcccgc
     5641 ccggctccgg gcggcacgtc cgggcctgga agcccgcgtc gtcgagctcg gcgtagagcc
     5701 gatccacggg cgcggccggc gcaggggcgg ccccctcccg gagggcgtaa tagcgctcga
     5761 ggccgtgctc gtggaggatc gcccagaccg cgcggcagcc ctcgcagcag aaccggcgcc
     5821 cggcttcgtc gccgtcggcg ccgggcggca ccggcagccc gcagtgggcg cacggcgcgc
     5881 ggacggcgcg cgccggcgcc acgctctcag tggtggccat ggcgctgctg cgtcgtggcc
     5941 cccgcgcccg ggtccacgga attgatccgg ccggcgatgg cgagcagccc caccacgatc
     6001 atcgcgatgg cgcagaccac cgggacgtgg cggcgcagcg ggccggcgag ggcctggagc
     6061 cccaggccca gccccagcat ggcgggcagc gtgcctaccc agaagacggc catgagcgcg
     6121 gcgcctccga gcgcggtgcc ggtgcccgcc gccgtggcga ggaacacgta gagccagccg
     6181 cacggcagga gcccggtggc gaggccgacc acgagggcgc ggaggggcgg cggcgcggcg
     6241 gcgacggcgc cgatgccgcg gccggcgagc gtgcggagcc ccgccggcgc cccgaggcgg
     6301 ggcacgcgca cgccccacgc ctcgaggagg ctccgggcgc cccagagagt gatgagcgcg
     6361 ccggagacga tcgcggcggt ccgctggatg ccgagcagga cgccgcccgt ggtgtcgagc
     6421 gccgccccgg ccgcgccggc ggcggccccc agcgcggcgt acacgccgag ccggcctccg
     6481 ttgtaggcca ggtgcgccag caggagcgcc cggcgccgcg cgcccggcgt ggtgccggtg
     6541 tagaagccga cgaagccgcc gcacatcccg gcacagtggg cgctgcccag caggctcgca
     6601 ccgagcacgg tcgcgatcag cacgctcatc gggaggcagc caggtcctgg ctgagcgtcc
     6661 gggtgaacac ttccccgctc cggctcacgc gcacgcgcag ctcccagagc ccgggatgcc
     6721 ccgccggcag cgccgccgcg tagacgcccg catcgccggt cggctcgagg gtcaggctca
     6781 cgatctcgcg tgcccgcgcg ctcggaaacg cctcgacggt cacggtggcg ccgccgaggg
     6841 ccgcgcccgc acgatcccgc accgccgcca ctagccgcgc cggccccgcg ccggctcgct
     6901 cgaggcgggc cgacaccgtc cagccgagct cggcgttgcg cgcctcctgc gccatgacct
     6961 gatcccactc gagcgccttg cggtagtagt ccggctccac cgcgaaggag gcgtcgcggt
     7021 tggccacgat catgaagccg atgttggccc ccgcgccgcc cacgagcagg gccaccacga
     7081 tcaccggcca gtaccagccc ctagcggcca tggtccttgc ccttgtcacg gtcgtggtcc
     7141 ttgtccttgt cgtggtcgtc ctcctccggg ccgagcaagc gatagggcac cacggtgctc
     7201 cagccggtcc cgtcgctgat ccggaggctc accgggcgcc ggccgtcctc gaaggccttg
     7261 tgtgaggccg tcacgaagat gggcgcggtc gccgacttgc ccgcggccac cggcagcggg
     7321 ttctccggcg cgatgagctc gagccgggcc ttcgggtcgg cggcgccctc cacgagctcg
     7381 accaggtagc ggtgctccac cgaatcccgg ttcacgatct tgacccggat ctggttcgac
     7441 acgagccccg agggcagcac ggtgaacggg ctgccgagcc cgcgcaggac cgtcacgtcc
     7501 gcgggcgccc ggtgggcgag cgcgtaggcg agcgtccccc agaccaggag cagcaggagc
     7561 gggtagacga ccacccgggg ccgcaggagc cgccgcggtt ccccggcgag ctcctcgcgg
     7621 gagctgtagc ggatcagcct gcggggccgc ccgatccggt ccatgatgga gtcgcacgcg
     7681 tcgatgcact gggtgcagtg gatgcactcc atctgcaggc cgtcgcggat gtcgatgccg
     7741 gtcgggcagc acgcgacgca ggcgccgcag tcgatgcaat cgccggcgtg cggggccttg
     7801 cccaccaggg tcaggccgga gcgcacgagc ttgctccgcg gctcgccgcg gcggcggtcg
     7861 tagccgacga tcagggagcg gcgatcgagc agcacggact gcaagcgccc gtaggggcag
     7921 gccaccatgc agacctgctc gcgcatgatg ccgaagtcca gcagcatcag cgcggtggtc
     7981 gccgccatca ccaggaaggc gatcggatgc tcgaagggcg accgctgcac ccagcgggcc
     8041 agctcgctca cgccgacgaa gtaggcgagg aaggtgtggg cgaggaacat gctgagggcg
     8101 aggaacaccg cgtgcttgag caggcgccgg ggacggaagc gcgaggcatc caggcgctcc
     8161 tgctcgcggg tgccgccctc gatgagccgc tcgatcggcc ggtacaggaa ctccatgtac
     8221 accgtctggg ggcacgccca gccgcaccag acgcggccgt gcaccgcggt gatcaggaag
     8281 atcgcgagga agaggccgac cagcaggagc atcaggagca cgctgtcggt gggcaggaag
     8341 gtcgccccga agagggtgaa ctcgcggtgg acgacgtcca gcaggatgag cggccgcttg
     8401 ttcatggaca gatacggaat gaccgtgaag agcgcgagca ggacccaggc gaccgcccgg
     8461 cgccgcttga agaagcggcc ggggaagagc cgcggccgca gccaccgacg cgtgccgtcg
     8521 ggattgagcg tcggcagcac gcgcccttcc gcctcgagcg gaaagctcac atcgtctctc
     8581 actggctggc cggcggcgct tccggcgcga cttcgccctt gctgttgacc ccctgcggcg
     8641 gcttggggtt cggcggcttg gtgccggaca gggtggccac gtacgcggtc atcgtgtcga
     8701 tctcggcggg cttgagctga tccttccacg ggatcatacc cttgtcggga atgccatcgt
     8761 ggatggtacg ccggatgtcg gcaagcgcgc ggccgtgcag ccagtagtcg tcggtgaggt
     8821 tcgggccgat gatcccctgg ccctcgggcc cgtggcaggg catgcagcgg accgcgaaca
     8881 tctccttgcc ggccgccatc gccttggcgt ccttcatgag ggcgcgcagc gcgtcctcgc
     8941 ccgcccccgc cgcgcccgcc gaaggcgcga gcctggcctg ctgctgggcg agctcgccca
     9001 tttcccgctc gtactgcgcg atcagggacg ggcccggccc gatctgatag tagaggtagt
     9061 agcccaccga gaacacgatg gtggcgagga agagccagtt ccaccagctg ggcagcggat
     9121 tgtcgtactc ctggattccg tcgtagtcgt gcgcgagcag ccggtcgcgg tcggtcgggc
     9181 tcatgcgcgg cctccccgag tcccggtggg agcggcgtcg tcggcgagcg gcatcaggct
     9241 cgcccgacgg aagggctcgc gattgcgcgc caggaacgtg ctcaccacga ccaccgcaaa
     9301 tgcggcgacc gcgacgatca gggcgatctg ggcgaacagg ttgaagtgca tcgcgctcat
     9361 gacgtcgccg agcttcatcg gccggtcccc tctccgcccg ccggggccgc ggccaccttg
     9421 ggcgagcgct tgatgtcggt gccgaggcgc tggagatagg cgatcagcgc caccagctgg
     9481 gtgtcctcga ggcccgcggg gcccccctgc tggacgatgg ccgccgcgac ctgctgggcc
     9541 tgcttgcggc cgagcgtggc cgcgccggcg acctcgtccc gcgtgtaggg cacgcccagg
     9601 gtcgcctgcg cggccacccc ccgctcgagg gccgcccagt tgatccgcgc gcgcgcgagc
     9661 cacggatagg ccggcatgat ggactgcggc gtcgtcgagc gcgggtcctc catgtggcgc
     9721 acgtgccaga ggtccggata cttgccgccc acccgcgcca gatcggggcc gatccgccgc
     9781 gatccccaga ggaacgggtg gtcgtagacg aactccccgg gcttgctgta ctcgccgtag
     9841 cgcatcgtct cggcgcggat gggccggatc atctgcgagt gacagttgtt gcagccctcc
     9901 gccacgtaca ggtcccgccc cgccagctcg agcggcgtgt aggggtgcac ggcctggatg
     9961 gtcggcacgt tcgagccgat gaggaacatc gggatgatct cgaagagcga ggcggtgacg
    10021 accgcgaccg ccacccacac cgagaaggtc agcggtaggc cttcccagcg ccgatgccag
    10081 cccatgcgcc ggaagccgcc gggggccgcc tcgggcgtgt aggcgggggc gagggcggga
    10141 gcctcgtgga cgggctcggc gtaccgggcc ggccgcccgc gccaggtcat gagcgtgttg
    10201 acgccgcaga ggatcagccc ggcgatgtag aggctgcccc cgagcacgcg cacccagtac
    10261 atcgggatca gccgcaccgt ggtctcgacg aagtccggat aggccaggcg cccggtctcg
    10321 tcgaaggcgc gccacatcag cccctgggtg aggccggccg catagatggc cacgatgtag
    10381 aggaggatgc cgacggtggc gatccagaag tgcagctcgg cgagccggcg gctccagagc
    10441 tcggcctgga acaggcgcgg caggagccag tagaccatcc cgaacaccat gaagccgacc
    10501 cagccgagcg cgcccgcgtg gacgtgggcg atcgtccagt ccgtgtagtg cgagagcgcg
    10561 ttgacgctct tgatcgagag cagcgggcct tcgaaggtcg acatgccgta gaaggtgacc
    10621 gcgaccacga agaacttgag gaccggatcc tcggtgacct tgtgccaggc cccgcgcagg
    10681 gtgagcaggc cgttgaccat cccgccccac gacggcatcc agagcatcac cgagaagagc
    10741 atgccgaggg tggaggccca cgcgggcagc gcggtgtagt gcaggtggtg cgggccggcc
    10801 cagatgtaga tgaagaccag cgtccagaag tgcaggatcg agaggcgata ggagaacacc
    10861 ggccggtccg ccgccttcgg catgaagtag tacatcatcc ccaggaacgg cgtggtgagg
    10921 aagaaggcca ccgcgttgtg gccgtaccac cactgcatga aggcgtcctg cacccccgcg
    10981 tagatcgagt agctcttgaa cggccccgcc accaccacca ggttgttgaa gatgtgcagc
    11041 accgcgatcg ccaccaccgt cgcgatgtag aaccagagcg ccacgtagag gtgccgctcc
    11101 cgccggcgga cgatggtgcc gaagaggttg accgcgaaga ccacccacac cagcgcgatc
    11161 gcgacgtcga cgggccactc gagctcggcg tactccttgg actgggtaat gccgaagggc
    11221 agcgtgaggg ccgccgccac gatgatcgcc tgccagcccc agaagtgcag gcggctcagg
    11281 ccgtcgctcc acatccgcgc cttcagcagc cgctgcgcgg agtagtagac gccggtgaag
    11341 atcgcgttgc ccgcgaaggc gaagatgacc gcgttggtgt gcagcggccg gagccggccg
    11401 aaggagagcc acgaggtctt gaagttgaag atcgggttgg tgagctggag cgcgatcacc
    11461 acgcccacca gcatgcccac cagcccccac acgagcgtcg cgagcaggaa gagccggacg
    11521 atcgcgtcgt cgtagacgaa ccgatccatc cgctgacccg ggaccgcgga accggccatc
    11581 gtcgcagtag tcaccgctgc actctccttg acagggttca catgtgaagc ctgtagcacc
    11641 gggtgggcag aagagcaatc cgggtgccag ccgagccgcg ccgcggcgtg gcgcaagtgc
    11701 atgtcgcgac tgatgctttc ggggagcgct cgccgcgccg cgggcaccga gaggccaggt
    11761 gaggcggcgg cgccccgtga agcgtggcgg cggcgcccca ggcggcgggc ggcgcgccgc
    11821 ccgtctcctc gtgcggccgg cgggccagga tgcggccggt caccgggcgc cggcccgccg
    11881 cagccgctcc accatcgcgg catggccccg ggcctcggcg agggcgagcg gcgtcctccc
    11941 gctccgatcg gccaggttca cgttcgcgcc ggcctcgatc agggcggcca gcgtctcggt
    12001 gtgacgccgg ccgccatcgc cgagcacgat cgactcgatc agcgcggtcc agccgaggtt
    12061 gttgacgtgg tcgagcggcg cgcccgcgcg gatgagcgtg cgcacgacct ccgcgtggcc
    12121 caggtgcgcg gccgcgatca gcgcggtgcc gtcgtagcgg ctggtcacgt tcctcgcgct
    12181 cgcgcccagt tcgagcgcaa gcttgagcgt cggcatgtcg ttggcgactg ccgcgatcgt
    12241 gacgatgtcg tagcggtcgc gctcgagcgc gttcggatcc gcgcccgcgg cggccagcgc
    12301 ccgcatggcg tcgtggtgcg cgccgaaggc ggccacgtgc aggggcgtgc gaccgtgcgc
    12361 atccc
//
LOCUS       DASUFA010000073        11850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_474800, whole genome shotgun sequence.
ACCESSION   DASUFA010000073 DASUFA010000000
VERSION     DASUFA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474800"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFX87_02855"
     CDS             complement(<1..639)
                     /locus_tag="VFX87_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX4993874.1"
                     /translation="MSPRVLRAIVAITTMMATFLVILDVTIANVALDHMRGSLSAGVD
                     EITWVLTSFLVANAISLPITGWLTDLLGRKRLFILATAAFTVTSAAVGAAPSLAVIVI
                     ARFLQGLAGGPLVPLSQSTMIETFPARQSGMAMAIWGIGIMFAPIIGPTLGGWITDNW
                     SWRWVFYINVPVGAAAVALAWLFVPESTTERPAVRRVDLPGLVLLVLGVAALQ"
     gene            complement(636..1847)
                     /locus_tag="VFX87_02860"
     CDS             complement(636..1847)
                     /locus_tag="VFX87_02860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX4993875.1"
                     /translation="MLAAGAYGVQTWRYWSRHVSTDDAFVEGHVSPVSARVRGTVLEV
                     LVRDNQEVPAGAPLARLDPRDLEVKVHQARAALATAAGRLHMATEGVPMTDESTRSQV
                     TRAEATVAGAALGIDSARRTLEERRSRLLARRASVQAAQADVAARQADFERARLDHRR
                     MRELLDRGLIARQEFDHAEAAFRTGEASVEATRQRVDVAHAEVAQAEAEVAAQEVALT
                     QSRRLLEAAEAALAEARSRRREVTIRGAEAVSVEAQVAEARADLREAELNLEYATITA
                     PVAGRVTRRTVEVGQTVQPGQPLLAIVDAARVWVIANYKETQLTHVRAGQRAWVSVDT
                     HPDLVFRARVDSIQSGTGSRFSLLPPENASGNFVKVVQRVPVKLVLEPGQNGHALLVP
                     GMSVVPTIELR"
     gene            complement(1925..3658)
                     /locus_tag="VFX87_02865"
     CDS             complement(1925..3658)
                     /locus_tag="VFX87_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4993876.1"
                     /translation="MRKTITGTGGMLLLQTLKDAGVEYLFTNPGSAETGLFAAIAEDG
                     DNRLVVGKHEGLVAAMADGYHRVSGKVGVVIAHVMGGSYQLAGQLFNAQVAGSSLLVI
                     AGDWASELQDYRGLAPFPGLSQAESMRPITKEARCAYQVHTNPKAISVATIRALREAT
                     TPPTGPVYLSISAELLHTEGLEATIGEGARYQIERPGPARAQTIAAIAKRLGEAQCPV
                     LMFGDDVWREGAQAEAVRLAEALEAPVFASRQIFQNFPTRHPLFCGNYPVSKDFEKAT
                     GLKPDLIFLVGCQGVHGSVNEPVIMQIGPNPLLMGRHYPLDLAAQCELPETLKGITQA
                     LGRVHGAEKAGAWATQRSKVRTYAAMLIEREENLVREHEHDSPVHPAVLEAHLASVLP
                     KNTVMVQESSTARTTLMAFGHGGMDWTRSGGGSLGFGVGAAIGAKIAVGRDRPVVLHL
                     GDGALGYSASGFWTMARYNTAVLTIVSNNESYQIVRHNWAKEMPDSKMIREGKYPGLW
                     LSAPATDHVALARSQGVDGEAVTTVKELEPALRRGMDKITRENRPYLIDVSVAREGIG
                     AESTWYQDWRL"
     gene            complement(3742..4167)
                     /locus_tag="VFX87_02870"
     CDS             complement(3742..4167)
                     /locus_tag="VFX87_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993877.1"
                     /translation="MNEPKQKFSASLAKDAVYKPGLRSFMEYRDLGIADATHGKMRAH
                     IVRVKQGGDTHDLHTTGLHKHECDFQMFYVLKGTIKFVYEGQGERTFGPGDCVLQPAG
                     IVHNELECTDDLEILEIYSPAVHPTVVVEKMPEAAAAAH"
     gene            complement(4188..4904)
                     /locus_tag="VFX87_02875"
     CDS             complement(4188..4904)
                     /locus_tag="VFX87_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4993878.1"
                     /translation="MNVVARMLTQEQDGMPGYMAHPDAPGQRPGILMVHHAHGVTADY
                     KIDAYRLACLGFNVLAPDLFNMFGIAGTNHIGMGADLQAKHGDDEFLGKMDEAWRYLI
                     DRMHTDPARTGCIGHCMGGRLLIPFAADHPSVKAIVLYYPSVREEPETSHRPRHAFHL
                     AKTLQCASLVFAAGLDYIATPAIQGPLWQSFIANGKLVEWHFFSDANHGFRHPDNPGF
                     QPYYADRTWPLTTSFLQRTV"
     gene            complement(4901..5731)
                     /locus_tag="VFX87_02880"
     CDS             complement(4901..5731)
                     /locus_tag="VFX87_02880"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4993879.1"
                     /translation="MIEVRRLGPQIGAEILGVDVRRLDDAGFARIYRAWLDHNVAVVP
                     GQELEIDDFLAYSRRFGQVVPHPSKMTRHPERPEITLLGVNKFGADGALDLAIYRRGA
                     EGWHTDGAYDEEPFKATQLYALAVPSRGGDTHFASMYAAYDALPPRLKERLEGRKGAF
                     TYGGRRKATALLNAEDRDWTPVFHPIIRTHPETGRRGLYFDPGKILYIDGLEPAESDA
                     LIDDLTERMIQPDAEYRHRWRKGDIVIWDNRCSYHKAAGDYPPEEDRIHWRVSIKERT
                     "
     gene            complement(5728..7962)
                     /locus_tag="VFX87_02885"
     CDS             complement(5728..7962)
                     /locus_tag="VFX87_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4993880.1"
                     /translation="MDARISRRGFLAAGGVLVVGLGLDGLGRAGAQSIPDADRFLGKS
                     LAPDAVDSFLAVHRDGTVTAFVGRVDLGTGGRIAMRQLVGEELDIPLERIAMIEGDTA
                     LTPNQGGTGGSYGIARGGSQLRWAAATARQALLALAAQRLGRPAGDLEVADGVVRAKD
                     GSGSVSYGELIGDRAFNLKVDAAAPLKPHDRFRFIGKSLPRPDLPAKITGHHRYLQDL
                     VLPNMLHARVIRPPAHGATLVSVDESSIAAIAGARVVRIQGFLAVVAEREWDALRAAG
                     ALKTQWGAGTGLPDHATEFEAMRASRVVRDQEIAKRGDLAALTAPPPGVRTLAASYRW
                     PMQTHGSIGPSCGVADVRADRVTVWSASQNVHGLQNTFARMLGVERDRVRVIYMDGAG
                     SYGANGADDAAAEAALLSRAVGRPVRVQWSRQEEHGLDPKGPAQLLDLRAAVDKDGEV
                     AAWETQAWLPIATANLPNIPLLAVDAAGLPQTPGRATGLIYQNVDPPYAMPNLNAVVH
                     WVADAPLRTSPLRAPGKIANTFAVESFTDEIAALARVDPLEFRLRRLSNPRGIEVLKR
                     VAARIGWQPRPSPRRMDRRAPVLTGRGLAYVHYKHEETIVAMGMEVAVERATGRIRVT
                     RVVAAQDCGLMINPDCVQAQLEGNILQTLSRALHEEVVYDRDRVTTVDWASYPILTFP
                     EVPKLELELIQRLDQPPLGAGEAASTPVGAALANAVFDATGVRLRTVPFRPERVKAAL
                     AGRS"
     gene            complement(7962..8438)
                     /locus_tag="VFX87_02890"
     CDS             complement(7962..8438)
                     /locus_tag="VFX87_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4993881.1"
                     /translation="MARLTLKVNGATREVESDDPDIPLLYVLRGDLGLTGTHFGCGLA
                     QCGACTVLVGGRAVRSCIAPAQSVVGQDVVTIEGLGSPERPDPLQAAFIAEQAAQCGY
                     CTAGMVMSARALLAQTPRPSEKQVRDALAGNLCRCGSHARVIRAVLRAAAAKPGAR"
     gene            complement(8438..9745)
                     /locus_tag="VFX87_02895"
     CDS             complement(8438..9745)
                     /locus_tag="VFX87_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993882.1"
                     /translation="MPLTDRYGLPVTTSSSTAFDRFQEGVDDLLAYGPGAEERFAAAL
                     AADERLALAHVGRALLAAVQGDAATARAAIARARETVDGATRRERQHVEAVSAFITGE
                     TARGLALVDEHVAEFPRDAMLVNQASSAIALAGRSDREAHRVAFLERLAPAYGADWWF
                     QSALAFTYHEIDRFDESRRLSQASLDQYPRNASAAHNLAHIAFETLDIEAGSVFLDDW
                     MTGYDRRAHFHCHLAWHQAMFALHEGRYAQALAFFERDILPASNPRSTMTDGSALLWR
                     VRLDAAGPAALPWRALADIAGRVSRPGYLFGECHAALAYAACGDHAALEKLMDGLRAL
                     DAKGNPIAGRVVLPLVQGTAAFAAGDFAGALAHFEPVQLAMHRIGGSHAQWELFEETM
                     VVCYLELARYDDALRLVRRRLARRASPRDRKWLARASARGTEA"
     gene            complement(9760..11103)
                     /locus_tag="VFX87_02900"
     CDS             complement(9760..11103)
                     /locus_tag="VFX87_02900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX4993883.1"
                     /translation="MPTNVIMPALELAQETGKVLRWIKAPGDTVRKGEPIVEIETDKV
                     TVEIESPASGILSGVTAQEGDVVPVGQTIALIAAAGEVPTATPASALSPQGRGQGEGP
                     KAEGPVKASPLARRIAEEHGVDLARIKTASGKIEKADVLAFVESRTMAPSNGAAVRLV
                     AASPKARRLAAERGVDLSALRGSGPGGAVLTVDIPVAPVAAPPLTRPLTTGPLTTGPL
                     TLPSPLRGEGVSTVWRIMAERMTASWTTAPHFYLVREVNVARLGAWLGTARKQTGAHI
                     TYSDLLVKLVAATLAQHPRVNVSWKDGALERHAEINVGLAVALEDGLVVPVIARADTL
                     GLKEIAARREDLVARAQAGKLRPADIQGGVFTISNLGMYGVDAFSAIVNPPQAAILAV
                     GRIADRVVPVNGQPAVQPTMTLTLSCDHRALDGARGAQFLGALADLIEEPLALLA"
     gene            complement(11155..>11850)
                     /locus_tag="VFX87_02905"
     CDS             complement(11155..>11850)
                     /locus_tag="VFX87_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4993884.1"
                     /translation="VNQAAKAHYMSGGKIKVPIVFRTTLGATRRSAAQHSQSLHAWVS
                     HVPGLKVALPSGPYEAKGLMKTAIRDDSPVVIFEDKMMYRVKGPVPEGDYTIPFGVAE
                     VKRVGKDITIVATSSMVGVALGAAKLLDEAGISAEVIDPRTTWPLDKQALIDSAKKTS
                     RALVVDEGYERYGVTAEIASVIAEGAFYYLDAPVKRMGAMDVPVPFSPVLEDLTVPSE
                     QTVFEAARALCRK"
BASE COUNT         1612 a         4264 c         4235 g         1739 t
ORIGIN      
        1 ctgcagcgcg gccacgccga gcacgagcag gacgaggccg ggcaggtcca cccgccgcac
       61 ggccggccgc tccgtcgtcg actccggcac gaacagccac gccagcgcca ccgcggcggc
      121 gccgaccggg acgttgatgt agaagaccca gcgccagctc cagttgtcgg tgatccagcc
      181 gccgagcgtg ggcccgatga tcggcgcgaa catgatgccg atgccccaga tggccatcgc
      241 catcccgctc tggcgcgccg ggaacgtctc gatcatcgtc gactgcgaga gcggcacgag
      301 aggcccgccg gccagcccct gcaggaaccg cgcgatcacg atgacggcca ggctcggggc
      361 cgcccccacc gcggccgagg tgaccgtgaa cgccgcggtg gccaggatga agagccgctt
      421 gcggccgagg aggtcggtca gccagccggt gatgggcagg ctgatcgcgt tggcgacgag
      481 aaacgaggtc agcacccacg tgatctcgtc caccccggcg gacaggctgc ctcgcatgtg
      541 gtcgagggcc acgttcgcga tcgtgacatc gaggatgacc aggaacgtgg ccatcatggt
      601 cgtgatggcc acgatcgccc gcaggacgcg cgggctcatc ggagctcgat agtggggacg
      661 accgacatgc cgggcacgag gagcgcgtgc ccgttctgcc cgggctcgag cacgagcttc
      721 accgggaccc gctggacgac cttgacgaag ttgccggagg cgttctcggg cggcagcagg
      781 gagaagcggg acccggtgcc gctctggatc gagtcgacgc gggcccggaa caccaggtcg
      841 ggatgcgtgt ccaccgagac ccacgcgcgc tggcccgcgc gcacgtgggt gagctgggtt
      901 tccttgtagt tcgcgatcac ccacacgcgg gctgcgtcca cgatcgcgag caggggctgg
      961 cccggctgca cggtctgccc cacctccacc gtccggcggg tgacgcggcc ggcgacgggc
     1021 gcggtgatcg tcgcgtactc gagattcagc tcggcctcgc gcaggtcggc gcgcgcctcg
     1081 gccacctgcg cctcgacgct caccgcctcc gcgccgcgga tcgtcacctc gcgccgccgg
     1141 ctgcgggcct cggcgagcgc cgcctcggcc gcctccagca gtcgccgcga ctgcgtcagc
     1201 gccacctcct gcgcagccac ctcggcttcg gcctgggcga cctcggcatg cgccacgtcc
     1261 acccgctgcc gcgtcgcctc cacggacgcc tcgccggtcc ggaacgcggc ctcggcgtgg
     1321 tcgaactcct gacgcgcgat cagcccgcga tcgagcagct cccgcatgcg gcggtgatcg
     1381 aggcgcgcgc gctcgaagtc cgcctggcgc gccgcgacgt cggcctgcgc cgcctggacg
     1441 gacgcccgcc gcgccaggag ccgactgcgc cgctcctcca gcgtccgccg ggcgctgtcg
     1501 atgccgagcg ccgcccccgc cacggtcgcc tccgcgcgcg tcacctggct gcgcgtggac
     1561 tcgtccgtca tgggcacgcc ctcggtggcc atgtggagcc ggcccgccgc cgtcgccagc
     1621 gccgcgcggg cctggtggac cttcacctcc aggtcccgcg ggtccaggcg cgccagcggc
     1681 gcccccgccg gcacctcctg gttgtcgcgc acgaggactt ccagaacggt gcctcgcaca
     1741 cgggcactga ccggcgagac gtggccctcg acgaaggcgt cgtcggtcga gacgtggcgg
     1801 ctccagtagc gccaggtctg gacgccgtac gcgccggcgg ccagcaccgc gaggccgatg
     1861 aggacgagga ccagcgcgcg ccagcgccgg cgcggtggaa aggcgtctcg cgagtcgaga
     1921 tcggtcagag ccgccagtcc tggtaccagg tggactcggc gccgatcccc tcgcgggcca
     1981 ccgacacgtc gatcaggtac ggccggttct cgcgcgtgat cttgtccatg ccgcggcgaa
     2041 gcgccggctc cagctccttc acggtggtca ccgcctcgcc gtccacgccc tgtgagcggg
     2101 ccagcgccac gtggtcggtg gcgggcgcgc tgagccagag gccgggatac ttgccctcgc
     2161 ggatcatctt gctgtccggc atctccttgg cccagttgtg ccgcacgatc tggtagctct
     2221 cgttgttcga cacgatcgtg agcaccgcgg tgttgtagcg cgccatcgtc cagaagcccg
     2281 aggcgctgta gccgagcgcg ccgtcgccca ggtgcagaac taccggtcga tcacgtccca
     2341 ccgctatctt ggcgccaatg gccgcaccta ctccgaagcc gagcgagccg ccgccgctgc
     2401 gcgtccagtc catgccgccg tggccgaacg ccatgagcgt ggtccgcgcc gtcgagctct
     2461 cctgcaccat caccgtgttc ttcggcagca cggaggccag gtgcgcctcc agcaccgcgg
     2521 ggtgcacggg cgaatcgtgc tcgtgctcgc gcaccaggtt ctcttcccgc tcgatgagca
     2581 tcgcggcgta ggtgcgcacc ttcgagcgct gggtggccca cgcgcccgcc ttctcggcgc
     2641 cgtgcacgcg gccgagcgcc tgggtgatgc ccttcaaggt ctcgggcaac tcgcactgcg
     2701 cggccaggtc cagtgggtag tgccggccca tcagcagcgg gttcggcccg atctgcatga
     2761 tgaccggctc gttcacgctg ccgtgcacgc cctggcagcc caccaggaag atcaggtccg
     2821 gcttgagccc ggtcgccttc tcgaagtcct tggacacggg atagttaccg cagaagagcg
     2881 ggtgccgcgt cgggaagttc tggaagatct gccggctcgc gaacaccggc gcctccagcg
     2941 cctcggccag ccgcaccgcc tccgcctgcg ccccctcgcg ccagacgtcg tcgccgaaca
     3001 tcagcaccgg gcactgcgcc tcgcccagcc gcttggcgat cgccgcgatc gtctgcgccc
     3061 gcgccggccc cggccgctcg atctgatacc gggcaccctc gccgatcgtc gcctcgagcc
     3121 cttcggtgtg cagcagctcg gcgctgatcg agaggtacac gggcccggtg ggcggcgtgg
     3181 tggcctcgcg cagggcgcgg atggtggcca cgctgatggc cttggggttg gtgtgcacct
     3241 ggtaggcgca gcgcgcttcc ttggtgatcg ggcgcatgga ctccgcctgg ctcagcccgg
     3301 ggaacggagc gaggccgcga tagtcctgga gctccgaggc ccagtccccc gcgatcacga
     3361 gcaaggagga gcccgccacc tgggcattga agagctggcc ggcgagctgg taggagccgc
     3421 ccatgacgtg cgcgatgacc acgccgacct tgccgctgac gcggtggtag ccgtcggcca
     3481 tcgcggccac caggccctcg tgcttcccca ccaccaaacg gttgtctcca tcttctgcaa
     3541 tggcggcgaa gaggccggtc tccgcggagc ccgggttggt gaagaggtac tccacgcccg
     3601 cgtccttgag cgtctggagg agcagcatgc cgccggtgcc ggtgatggtc ttgcgcatgg
     3661 ttggcctcgg tgttgtcggt tcgagagggc cccctcaccc caccctctcc cccggaaggg
     3721 gagagggtga gagatgggcg tctagtgcgc ggcggccgcg gcctcgggca tcttctccac
     3781 gaccacggtc gggtgcaccg cgggcgagta gatctccagg atctcgaggt cgtcggtgca
     3841 ctcgagctcg ttgtggacga tgccagcggg ctggagcacg cagtcgccgg ggccgaaggt
     3901 gcgctcgccc tggccctcat agacgaactt tatcgtgccc ttgaggacgt agaacatctg
     3961 gaagtcgcac tcgtgcttgt gcagcccggt ggtgtggaga tcgtgggtgt cgccgccctg
     4021 cttcacgcgg acgatgtggg cgcgcatctt gccgtgggtg gcgtcggcga tgccgaggtc
     4081 gcggtactcc atgaagctgc ggaggccggg cttgtagacg gcgtccttcg ccaggctcgc
     4141 gctgaacttc tgcttgggct cgttcatgtc ggtctctcct ctcgcgatca gacggtgcgc
     4201 tgcaggaagc tggtggtcag cggccaggtg cggtcggcgt agtagggctg gaaacctggg
     4261 ttgtcggggt ggcggaagcc gtggttggcg tcggagaaga agtgccactc cacgagcttg
     4321 ccattggcga tgaagctctg ccagagcggg ccctggatgg cgggcgtcgc gatgtagtcc
     4381 aggcccgccg cgaagaccag cgaggcgcac tgcagcgtct tggccaggtg gaaggcgtga
     4441 cggggccggt gcgaggtctc gggctcttcg cgcaccgagg ggtagtagag cacgatcgcc
     4501 ttcaccgacg ggtggtcggc cgcgaacggg atcagcaggc gcccgcccat gcagtggccg
     4561 atgcagccgg tgcgcgccgg atcggtgtgc atgcggtcga tcaggtagcg ccaggcctcg
     4621 tccatcttgc ccaggaactc gtcgtcgccg tgcttggcct gcagatcggc gcccatgccg
     4681 atgtggttgg tgcccgcgat gccgaacatg ttgaagagat cgggcgcgag cacgttgaag
     4741 ccgaggcacg cgaggcggta ggcgtcgatc ttgtagtcgg cggtgacgcc gtgggcgtga
     4801 tggaccatca ggatgcccgg ccgctggccg ggcgcgtccg gatgcgccat gtagcccggc
     4861 atgccgtcct gctcctgcgt gagcattctc gcaacgacgt tcatgtccgc tccttgatcg
     4921 agacgcgcca gtggatgcgg tcttcctccg gtggatagtc gcccgccgcc ttgtggtacg
     4981 agcagcggtt gtcccagatc acgatgtccc ctttgcgcca ccgatgccgg tactcggcgt
     5041 cgggctggat catgcgctcg gtcagatcgt cgatcaacgc gtcgctctcc gccggctcga
     5101 ggccgtctat ataaaggatc ttgcccgggt cgaagtagag gccccggcgg ccggtctccg
     5161 ggtgcgtgcg gatgatcggg tggaagaccg gcgtccagtc gcggtcctcc gcgttgagca
     5221 gcgccgtcgc cttccggcgc ccgccgtacg tgaaggcccc tttgcgaccc tccaggcgct
     5281 ccttcaaccg gggcggcagg gcgtcgtagg ccgcgtacat gctggcgaag tgcgtgtcgc
     5341 cgccgcggct cggcaccgcg agcgcgtaga gctgggtggc cttgaagggc tcctcgtcgt
     5401 aggcgccgtc ggtgtgccag ccctccgccc cgcggcggta gatcgccagg tcgagcgcgc
     5461 cgtccgcgcc gaacttgttc acgccgagca gggtgatctc ggggcgctcc gggtgccgcg
     5521 tcatcttcga cgggtgcggg acgacctggc cgaaccggcg gctgtaggcc aggaaatcgt
     5581 cgatctcgag ctcttgcccg gggaccaccg ccacgttgtg gtcgagccac gcgcggtaga
     5641 tccgggcgaa ccccgcgtcg tcgagcctcc ggacgtccac gcccaggatc tcggcgccga
     5701 tctgcgggcc cagccggcgc acctcgatca tgagcgtccc gcgagggcgg ccttcacccg
     5761 ctcgggccgg aacggcaccg tgcgcaggcg caccccggtc gcgtcgaaca ccgcgttggc
     5821 gagcgcggcg cccaccggcg tggaggccgc ctcccccgca cccagcggcg gctggtcgag
     5881 gcgctggatc agctcgagct cgagcttggg cacctccggg aacgtgagga tcggatagga
     5941 cgcccagtcc accgtggtga cccggtcccg atcgtagacg acctcttcgt gcagggcgcg
     6001 cgagagcgtc tgcaggatgt tgccctcgag ctgggcctgc acgcagtccg ggttgatcat
     6061 gagcccacaa tcctgggccg ccaccacccg ggtcacccgg atgcggccgg tagcccgctc
     6121 caccgccacc tccatgccca tcgccacgat cgtctcctcg tgcttgtagt gcacgtaggc
     6181 gagcccgcgg ccggtgagga ccggcgcccg ccggtccatc cttctcggag acggccgcgg
     6241 ctgccagccg atgcgcgccg cgacgcgctt caggacctcg atgccgcgcg gattggagag
     6301 ccggcgcagc cggaactcca gcgggtccac gcgggccagc gcggcgatct cgtcggtgaa
     6361 cgactccacc gcgaacgtgt tggcgatctt gccgggcgcc cggagcggcg aggtgcggag
     6421 cggcgcgtcc gccacccagt gcaccaccgc gttcaggttc ggcatcgcgt agggcggatc
     6481 cacgttctgg tagatcagcc cggtggcgcg gcccggcgtc tgagggagac ccgccgcgtc
     6541 cacggccagg agcgggatgt tggggagatt ggcggtggcg atgggcagcc acgcctgcgt
     6601 ctcccacgcg gccacctcgc catccttgtc caccgcggcg cgaaggtcca ggagctgggc
     6661 ggggcctttc gggtcgagcc cgtgctcctc ctggcggctc cactgcacgc gcaccgggcg
     6721 gccgaccgcc cgggagagca gcgccgcctc cgcggccgcg tcgtcggccc cgttggcgcc
     6781 ataggagccc gcgccatcca tgtagatgac gcgcacccgg tcgcgctcga cgccgagcat
     6841 ccgcgcgaag gtgttctgca gcccgtgcac gttctgcgac gcgctccaca ccgtgacgcg
     6901 gtcggcccgc acgtcggcca caccgcagga gggcccgatg gagccgtggg tctgcatggg
     6961 ccagcggtaa gaggccgcca gggtgcgcac gcccggcggc ggcgcagtca gcgcggccag
     7021 atccccacgc ttggcgatct cctggtcgcg gacgacccgg ctcgcccgca tcgcttcgaa
     7081 ctccgtggcg tgatcgggca gaccggtgcc cgccccccac tgagtcttca gcgcgcccgc
     7141 cgcgcgcagc gcatcccact cgcgctcggc gacgaccgcg aggaagccct ggattcgcac
     7201 cacgcgggct cccgcgatcg ccgcgatcga cgactcgtcc accgagacga gcgtggcgcc
     7261 gtgcgcgggc gggcggatca cgcgggcgtg aagcatgttc gggagcacca ggtcttgcag
     7321 atagcgatga tgcccggtga tcttcgcggg caggtcggga cgcggcagcg acttgccgat
     7381 gaaccggaag cgatcgtggg gcttcaacgg cgcggcggcg tccaccttca ggttgaacgc
     7441 gcgatcgccg atcagctcgc cgtagctcac cgacccgctg ccgtccttgg cgcgcaccac
     7501 gccgtcggcg acctcgagat cgccggcggg gcgacccagg cgctgggccg ccagggccag
     7561 cagggcctgc cgcgcggttg cggccgccca gcgaagctgc gacccgccgc gggcgatgcc
     7621 gtagctgccc ccggtcccgc cctggttggg cgtcagcgcg gtgtcgccct cgatcatcgc
     7681 gatgcgctcg agcgggatgt cgagctcctc gcccacgagc tggcgcatcg cgatgcgccc
     7741 acccgtgccg aggtccaccc ggccgacgaa ggcggtgacc gtcccgtcgc ggtgcacggc
     7801 caggaacgaa tccaccgcgt cgggggccag ggacttgccg aggaagcgat ccgcgtcggg
     7861 aatggactgc gcccccgccc ggccgagccc atcgagcccc aggccgacga ccaacacccc
     7921 gcccgcggcg aggaagccac gccgggagat gcgcgcgtcc atcaacgggc ccccggcttc
     7981 gcggcggccg cgcggagcac cgcgcggatc acgcgcgcgt ggctgccgca gcggcagagg
     8041 ttaccggcca gcgcgtcgcg cacctgcttc tcgctcggcc gcggcgtctg cgccaggagg
     8101 gcgcgcgcgc tcatgaccat gccggccgtg cagtagccgc actgggcggc ctgctccgcg
     8161 atgaacgcgg cctggagcgg atccggacgc tcgggcgagc ccaggccctc gatggtcacc
     8221 acgtcctgcc cgaccaccga ctgcgccggc gcgatgcacg agcggacggc gcggccgccg
     8281 acgaggacgg tgcacgcccc gcactgggcg aggccgcagc cgaaatgcgt gccggtcaac
     8341 cccagatctc cccgcaggac gtacaggagc gggatgtcgg ggtcgtccga ctcgacctcc
     8401 cgggtcgcgc cgttcacctt gagcgtcagc ctggccatca ggcctcggtg ccgcgggcgc
     8461 tcgcccgcgc gagccacttc cggtcccgcg gtgaggcgcg gcgcgcgaga cgccggcgca
     8521 ccaggcgcag ggcgtcgtcg tagcgcgcga gctcgaggta gcacacgacc atggtctcct
     8581 cgaagagctc ccactgggcg tggctgccgc cgatgcggtg catcgcgagc tgcaccggct
     8641 cgaagtgggc cagcgctccc gcgaagtcgc ccgccgcgaa cgcagccgtc ccctggacca
     8701 gcggcagcac gacccggccc gcgatcgggt tgcccttcgc gtccagcgcc cgcaggccgt
     8761 ccatcagctt ctcgagcgcg gcgtggtcgc cgcacgcggc gtaggccagc gcggcgtggc
     8821 attcgccgaa gaggtagccg ggccgcgaga cgcgccccgc gatgtcggcg agggcccgcc
     8881 acggtagcgc cgccgggccc gccgcgtcga gccgcacgcg ccagagcagc gcgctgccgt
     8941 cggtcatggt ggagcgcggg ttgctcgcgg gcaggatgtc gcgctcgaag aacgcgagcg
     9001 cctgcgcgta gcggccctcg tgcagggcga acatcgcctg gtgccaggcg aggtggcagt
     9061 ggaagtgggc acgccggtcg tacccggtca tccagtcgtc caggaacacc gaccccgcct
     9121 cgatgtcgag cgtctcgaag gcgatgtggg ccaggttgtg ggcggcgctc gcgttgcgcg
     9181 gatactgatc gagcgacgcc tgggagagcc ggcgcgactc gtcgaagcgg tcgatctcgt
     9241 gataggtgaa ggcgagcgcc gactggaacc accagtcggc cccgtaggcg ggggccaggc
     9301 gctccaggaa ggccacgcgg tgtgcctcgc ggtcgctccg cccggccagc gcgatcgcgc
     9361 tgctcgcctg gttgacgagc atcgcgtcgc gcgggaactc ggccacgtgc tcgtccacca
     9421 gcgcgaggcc acgcgcggtc tctccggtta tgaacgcgct caccgcctcg acgtgctgcc
     9481 gctcgcgccg cgtggcgcca tccacggtct cgcgggcgcg cgcgatggcg gcgcgcgccg
     9541 tcgccgcgtc cccctgcacc gcggcgagga gcgcgcgccc cacgtgcgcc agcgcgagcc
     9601 gctcgtcggc ggcgagcgcg gccgcgaagc gctcctcggc gccggggccg taggccagca
     9661 ggtcgtccac gccctcctgg aatcgatcga aggcggtgga cgacgacgtg gtcacgggca
     9721 atccgtagcg gtcggtcaga ggcatggggt gtccgcgtct caggctagca gggccagggg
     9781 ctcctcgatc aggtccgcga gcgcgcccag gaactgggcg ccgcgggcgc cgtcgagggc
     9841 acggtggtcg caggacagcg tcagcgtcat cgtcggctgc acggccggct ggccgttcac
     9901 gggcaccacg cggtcggcga tgcggccgac ggccaggatc gcggcctgcg gcggattgac
     9961 gatggcgctg aaggcgtcca cgccgtacat gccgagattg ctgatcgtga acacgccgcc
    10021 ctggatatcc gccggccgaa gcttgccggc ctgggcgcgg gccaccaggt cctcgcggcg
    10081 ggccgcgatc tccttgaggc cgagcgtgtc ggcgcgcgcg atgaccggca ccaccaggcc
    10141 atcctccagc gcgaccgcga ggccgacgtt gatctcggcg tgccgctcga gcgcgccgtc
    10201 cttccacgag acgttgacgc gcggatgctg cgccagcgtg gccgccacca gcttcacgag
    10261 caggtccgag tacgtgatat gcgccccggt ctgcttgcgg gcggtcccga gccacgcccc
    10321 cagccgcgcc acgttgacct cgcgcaccag gtagaagtgc ggcgccgtgg tccacgacgc
    10381 ggtcatccgc tccgccatga tccgccacac ggtgctcacc ccctctcccc tcaggggaga
    10441 gggcagggtg aggggtccgg tggtgagggg tccggtggtg aggggtctgg tgaggggcgg
    10501 cgcggccacc ggcgccaccg gaatatccac ggtgagcacc gcgccccccg gccccgaccc
    10561 ccgcagcgcg ctcagatcca ccccgcgctc cgccgcgagc cgccgcgcct ttggcgacgc
    10621 ggccacgagc cgcaccgccg cgccattgct cggcgccatc gtccggctct ccacgaacgc
    10681 gagcacgtcc gccttctcga tcttgccgct ggcggtcttg atgcgcgcga ggtccacgcc
    10741 gtgctcctcg gcgatccggc gggctagcgg ggaagctttc acgggcccct cggccttggg
    10801 cccctcaccc tgccctctcc cctgagggga gagggcagat gcgggggtgg cggtggggac
    10861 ttcgcccgcg gcggcgatga gggcgatggt ctggccgacc gggacgacgt cgccttcctg
    10921 ggcggtgacg ccgctcagga tgccggaggc gggggactcg atctcgaccg tgaccttgtc
    10981 ggtctcgatc tcgacgatgg gctcgccctt ccgcacggtg tcgccgggcg ccttgatcca
    11041 gcgcaggacc ttgccggtct cctgcgccag ctccagcgcg ggcatgatga cgttggtcgg
    11101 catcgttaga ccgctcccct caccctgccc tctccccagc ggggagaggg ttctttactt
    11161 gcgacatagc gcgcgggcgg cctcgaacac ggtttgctcc gaggggaccg tgaggtcctc
    11221 cagcaccggg gagaagggca ccggcacgtc catcgcaccc atgcgcttca ccggcgcgtc
    11281 gaggtagtag aacgcgccct ccgcgatgac cgaggcgatc tcggcggtga ccccgtagcg
    11341 ctcgtagccc tcgtccacca cgagggcgcg cgaggtcttc ttggccgaat cgatcagcgc
    11401 ctgcttgtcg agcggccacg tggtccgcgg gtcgatcacc tccgcgctga tgccggcctc
    11461 gtccaggagc ttggccgcgc ccagagcgac gcccaccatg ctgctggtgg ccacgatcgt
    11521 gatgtccttg cccacccgct tcacctcggc caccccgaac gggatcgtgt agtcaccctc
    11581 gggcaccggg cccttcaccc ggtacatcat cttgtcctcg aagatgacca ccgggctgtc
    11641 gtcgcggatc gcggtcttca tgagcccctt ggcctcgtag gggccggagg gcagcgccac
    11701 cttgagaccg ggcacgtggc tcacccaggc gtggagcgac tgcgagtgct gggcggccga
    11761 cctgcgggtg gcgccgagcg tggtgcggaa gacgatcggc accttgatct tgccgcccga
    11821 catgtagtgc gccttggcgg cctggttgac
//
LOCUS       DASUFA010000074        11680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1270111, whole genome shotgun sequence.
ACCESSION   DASUFA010000074 DASUFA010000000
VERSION     DASUFA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270111"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /gene="tcuA"
                     /locus_tag="VFX87_02910"
     CDS             complement(<1..1464)
                     /gene="tcuA"
                     /locus_tag="VFX87_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX4993885.1"
                     /translation="MGHDVVVIGGGNAALCAALAAREGGASVLVLEKAPEAERGGNSF
                     FTAGGFRFAHQGLEDLRRDVIPDLSDEEARTIAIPAYTEDQYYDDIMRVTEGRSDLDL
                     AQHLVQRSKPTVMWMRTQGIRWILMFSRQSYKVGDRHHFWGGLNLEAVGGGPGLVEAL
                     FERAAKVGIEVRYGVAGHKLIQDGSGRVTGVRTRSAAGTSEIPCRAVVIASGGFEANP
                     EWRTRYLGPNWDLARVRGTRHNVGEGIAMALEIGAQAYGHWSSCHAVAWDLNAPAFGD
                     RRVGDMFQKHSYPLGLIVNVRGERFVDEGADFRNYTYAKYGREILRQPQEAAFQIFDQ
                     KTVELLREEYRIREITKAEAGTIEELAKKLEVNPEGLSRTVRDFNAAVMDQVPFNPAV
                     LDGRGTRGITPPKSNWAQRLDSPPYVGFGVTCGITFTFGGLRVDTRGQVLDTWDRPIR
                     GLYAAGELVGGLFYHNYPGGTGLMAGAVFGKTAGDSVA"
     gene            complement(1493..2281)
                     /locus_tag="VFX87_02915"
     CDS             complement(1493..2281)
                     /locus_tag="VFX87_02915"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX4993886.1"
                     /translation="MPAWLAVTILGIIEGVTEFLPISSTGHLLLVENAGWVPRQSDLF
                     NITIQSGAALAVLLAFGGRLRDLLGRLHEPAARAYLLKLALAFAITGVGGLAVKRLGF
                     ALPKETAPIAWATLIGGVAILAVEWSLRARPSVSHVTWAMAVAVGLGQLLAAVFPGTS
                     RSAATILIVMLFGLSRPVATEFSFLVGVPTLLAAGGLETLQALRHPAGPPTDWGLLAL
                     GTLVSAVTAFVAVRWLLRWIQTHSFASFGWYRVALGLVLLTRLG"
     gene            complement(2289..3107)
                     /locus_tag="VFX87_02920"
     CDS             complement(2289..3107)
                     /locus_tag="VFX87_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4993887.1"
                     /translation="MTTDASPITLRGWALILGASSGFGAATSLALARAGLDVFGVHLD
                     RKSTLPNVERLASEIKSLGREAHFFNVNAADEEKRAEVATEMERVLRERNQVGQVRVM
                     LHSLAFGTLKLFVADPMKEAVTKAQMDMTLDVMAHSLVYWAQELVGRGLMSRGGRIYA
                     MTSAGGARVLPYYGPVSAAKAALESNIRQLAAELAPQGITANSIRAGVTATPAAQKIP
                     NYEEFSQQAARRNPHRRLTTTDDVARAIVVLSHPDTHWITGNVIGVDGGEEIVG"
     gene            3192..3923
                     /locus_tag="VFX87_02925"
     CDS             3192..3923
                     /locus_tag="VFX87_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX4993888.1"
                     /translation="MAKTAGILLIGNEILSGKITDANAAYLCRELRGLGVEVRRITVI
                     PDEVDLIAAEVRAFSAAFDVVFTSGGVGPTHDDVTIEGVARALGVPVIREPRLVRLLE
                     GYYKGRLNDARLKMAEVPEGAELTADGELVFPAVVVRNIYILPGVPEIFRQKFDALKE
                     RFRDAPFHLCCVYVSMGEGTLADHLNALLAGHPDLLLGSYPEFSNPEYKVKVTLESKD
                     RGYLDDAITDFLGRLPQDAVVRVDR"
     gene            3991..5673
                     /locus_tag="VFX87_02930"
     CDS             3991..5673
                     /locus_tag="VFX87_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4993889.1"
                     /translation="MARLGAAPFLDPPEGIHAEIAWEMLRGAGWITPHLNGVPYFDKP
                     PLPYWLMAGAFAWLGPTETAARLVSALATVAVAVLTALIGTRLASGRVGLMAGLMVAA
                     NLEVFLFGRMVKPDMLLVACILLGLYGFILAYLGGGRWPLLLFYGGLGLAVLAKDFLG
                     AVGPLAIVALFLWLTREGGSWRQWAPLSAILLLLAVAVPWYLLVESQYRGFLWYTVVD
                     NHVLNVARQRVFPDEDVPLSTLEFLGVTALGFFPWVLALPLAVARVIRRRPWKTPEAR
                     VWMLLALWTVGILGVFALSPFKLPHYGLPAFPAMALLVAKLWDEAIERPPGAPRSFSL
                     LVPALLATALVGTATLGAWLGWLRLSPEAVAVADVSARNMAAQGQVATSDFLAQFRPL
                     FGPIAGIFLLTTAALAVAAWRRLPRLGLGALLAAMIAFLPLSVEGLTLFAKSRSVRLM
                     TDAILLRAGPGDVLAHEGAIENSASGLLRLDRRVQIVNGLQSNLAFGSTFAEARPRFW
                     DSAALAHAWAGDRRVFLLSAATPSRSVVRELPADHVHLLVEGGSRRLYSNRP"
     gene            5711..5947
                     /locus_tag="VFX87_02935"
     CDS             5711..5947
                     /locus_tag="VFX87_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993890.1"
                     /translation="MDAASQLRQAHRTTALITGGLITGLVMYAAIAGFVLVILGGPFL
                     DFYLVGSRSLALLHVNAPRYETWEAWLATPAPRP"
     gene            5917..6633
                     /locus_tag="VFX87_02940"
     CDS             5917..6633
                     /locus_tag="VFX87_02940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4993891.1"
                     /translation="MAGHAGAAALTVGVSPYRLKDDPHSSHSVILSRLGEGRGRKALD
                     VGAADGFLAERLTAQGWSVTALERDPELAARARGRCKEVVVADLESAPPLLHGPFEAI
                     VYGDVLEHLSDPGAALRALDQTLAPEGTVIVSVPNVAHLWVRLSLLAGRFDYADRGIL
                     DRTHLRFFTRRTLLDLLRSAGLSVVELAVTPVPLPLVVSPRWRGRWLSRVHALSAGAA
                     RGWPGGLAYQFVAVCRARTS"
     gene            6634..7452
                     /locus_tag="VFX87_02945"
     CDS             6634..7452
                     /locus_tag="VFX87_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4993892.1"
                     /translation="MAGPFSKGAPKVVVVMPAYNAGRTLRMTYEELPKDTVNLVILVD
                     DGSTDATLEVARDLGLEIFVHDKNYGYGANQKTCYTEALKAGADIVVMVHPDYQYDPT
                     LVPQIIAPIARGEADLVLGSRLAGGGSAIEQGMPWWKYVANRFLTGVENMAFGLHLSE
                     YHTGYRAFSRGVLDAVNFRMNSDGFVFDQEIVAQAVATGFRIAEIPVPVRYFPEASSA
                     SFSASCVYGLKILWVVARYLLHRTGLKRSRRLGSIAGRYSRLGRPGSPPPADSR"
     gene            7449..9017
                     /locus_tag="VFX87_02950"
     CDS             7449..9017
                     /locus_tag="VFX87_02950"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX4993893.1"
                     /translation="MIVVRLLDGAAVLAAAGLGLALAFWTGRREHWLWRPEHLFLLLL
                     GLVALRVWLAPRPLPAVRPRRAVLVGVVAYAVVFSFVTVTRHLTFATHALDLGYYVQL
                     VWNLARGAGPYVSLPEMHAWGDHLSPIMYVFVPAYWLVPGPVVLLVAQSVALALGGFA
                     VFGLARARLGDERPAAAFALLYLVNPSLHGINVRDFHAAALAIPLLLAAFWAVETGRP
                     WLALAPAALTLLCREDAALPVMGLGAWMAAAHRRWLAGVVTATVALALLAVDVRWIIP
                     AYRGEPYSHLGRYAHLGGSLAEIIVNALVHPWRTLATLGTVDRAVYLAAMLAPLAFLP
                     LLGGWDLLGALPALAQNLLSRDPVLYGFRTQYQSFVLPFLILAAIGGYARLARRRPGA
                     WPAAVLVVAMVVSLALASRTVNNLAIARFWPVPEQRAAYQVLARVPPAAAISAQDPYV
                     PHLSLRPLVFVFPVGLEKSDYVLVNLDSYPWRNLPDVTLTRDGSRVTIVAGGVERRYA
                     VAAQGGPHLLLRRL"
     gene            complement(9018..9353)
                     /locus_tag="VFX87_02955"
     CDS             complement(9018..9353)
                     /locus_tag="VFX87_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993894.1"
                     /translation="MEPVKDASHLYEVERRARHAERPGFRITELQIGPRQQVPWHYHT
                     SIRDTFYVLEGRLRLFLRDPKEEIRLEPGDSYAVRAGRPHLVTNGGETSAVFLVLQGV
                     GDYDYVPLT"
     gene            complement(9374..9790)
                     /locus_tag="VFX87_02960"
     CDS             complement(9374..9790)
                     /locus_tag="VFX87_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4993895.1"
                     /translation="MTADHERADLAEANARFYRVFETLDLNQMDEIWAHEDHVRCVHP
                     GWCMLAGWEAVRHSWEVIFKNSQEMRFSITDVHAHVAGGFGWVSCTENILSESRGNIS
                     VTSVLATNIFERSGGEWLLIHHHASHILTGDPSAGA"
     gene            9883..10830
                     /locus_tag="VFX87_02965"
     CDS             9883..10830
                     /locus_tag="VFX87_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4993896.1"
                     /translation="MPASPLAVEARDLVKTFRSGWLRRRETAALRGASLQVPRGAIYG
                     LLGPNGAGKTTLLSILATLLLPDSGSARILGHDVVREAFAIRRRLNMASGNASFVWSL
                     RPAEVLAFYGRLYGLHGRALKCRVYELVERFELTPHLKTEYNELSTGLKQRLALAKAL
                     LNDPEVLFLDEPTLGLDPDVSIRIRAQIAELRRERDTTIILTTHYMREAEELCDRIAF
                     IKGGRILAEGSADELKRQIRIGDVIALRLEPAVPVGLAELPGVMGVQARGDRIDCTVD
                     SSEKRLPDILKWLCDHGVLIRDCQVREPDLEEVFVELSR"
     gene            10814..11647
                     /locus_tag="VFX87_02970"
     CDS             10814..11647
                     /locus_tag="VFX87_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4993897.1"
                     /translation="MSSRAEPLPTTVAESWAAEWARLTAFAYRNLIMARRNVFFVFEL
                     TFWPGVAMLSHGLLTRFLDLTPEMTAFILVGTVALSAVQVCQLDVAYAVLFDIWSKSM
                     KHQFLTPVRIHHLALGSWLVGVGRGVIVWALMAVIGGRAFGFDFLGPGPLRVALFLLG
                     CFLTALVIGLLVCALVLLYGTRAETSAWAAVNFFVMLGGIYYPISVLPGWVQAISAGI
                     PLTYFLDGIRVGYGFPSQFAYPFATGFALSLLYAVLAHWALAAAVTRSRRTGLLLKLS
                     E"
BASE COUNT         1492 a         4268 c         4027 g         1893 t
ORIGIN      
        1 tgcgacggag tcgcccgcgg tcttgccgaa gacggcaccc gccatgaggc cggtgccgcc
       61 cgggtagttg tggtagaaga gcccgcccac cagctcgccc gccgcgtaga ggccccggat
      121 gggccggtcc cacgtgtcga gcacctggcc gcgcgtgtcc acgcgcaggc ccccgaaggt
      181 gaaggtgata ccgcaggtca ctccgaagcc cacgtagggc gggctgtcga ggcgctgcgc
      241 ccagttggac ttgggcgggg tgatcccgcg ggtgccgcgg ccgtcgagca ccgccgggtt
      301 gaacggcacc tggtccatga ccgccgcgtt gaagtcgcgc accgtgcggc tgagcccttc
      361 ggggttgacc tcgagcttct tggccagctc ctcgatggtg cccgcctccg ccttggtgat
      421 ctcgcggatg cggtactcct cgcgcagcag ctccaccgtc ttctggtcga agatctggaa
      481 ggcggcctcc tgcggctgcc gcaggatctc gcgcccgtac ttggcgtagg tgtagttgcg
      541 gaagtcggcg ccctcgtcca caaagcgctc gccgcgcacg ttgacgatca gcccgagcgg
      601 gtacgagtgc ttctggaaca tgtcgcccac gcgccggtcg ccgaaggcgg gcgcgttcag
      661 atcccacgcc accgcgtggc aggacgacca gtggccgtag gcctgcgcgc cgatctcgag
      721 cgccatcgcg atgccctcgc ccacgttgtg gcgggtgccg cgcacgcgcg ccaggtccca
      781 gttgggcccg aggtagcgcg tgcgccattc cggattggcc tcgaagccgc ccgaggcgat
      841 gaccaccgcg cggcagggga tctcgctcgt gcccgccgcg ctgcgcgtcc gcacgccggt
      901 cacgcgcccc gagccatcct ggatcagctt gtgcccggcc accccgtagc gcacctcgat
      961 gccgaccttg gccgcccgct cgaagagcgc ttccaccaga cccggcccgc ctcctacggc
     1021 ttccaggttg agcccgcccc agaagtggtg gcggtcgccc accttgtagg actggcggct
     1081 gaacatgagg atccagcgga tcccctgggt gcgcatccac atcacggtgg gcttcgagcg
     1141 ctggaccagg tgctgggcga ggtcgagatc ggagcggccc tcggtcaccc gcatgatgtc
     1201 gtcgtagtac tggtcttccg tgtaggcggg gatcgcgatg gtccgcgctt cctcgtcgct
     1261 cagatccggg atgacatcgc gccggagatc ctcgagaccc tggtgggcga agcggaaccc
     1321 gcccgcggtg aagaaggagt taccgccccg ctcggcctcg ggcgccttct ccagcaccag
     1381 caccgaggcc ccgccctcgc gcgcggccag cgccgcgcag agagccgcat tgccgccgcc
     1441 gatcacgacc acgtcgtgcc ccatcgcgtc gactacctcc agtcaggccg gctcagccga
     1501 gccgggtcag caggacgaga ccgagagcca cccggtacca tccgaacgag gcgaagctgt
     1561 gcgtctggat ccagcggagc agccaccgca ccgccacgaa ggcggtcacc gcggagacca
     1621 gcgtgcccag cgccagcagc ccccagtcgg tcggcgggcc cgcgggatgc cggagcgcct
     1681 gcagcgtctc gaggccgccc gccgcgagca gggtaggcac gccgaccagg aacgagaact
     1741 cggtcgcgac gggtcggctc aggccgaaca gcatgacgat caggatcgtg gcggccgagc
     1801 gcgacgtgcc ggggaagacc gcggcgagga gctgccccag cccgaccgcc accgccatcg
     1861 cccaggtcac gtgcgacacg gacggccgcg cgcgcagcga ccattccacc gccaggatcg
     1921 ccacgccgcc gatcagggtg gcccacgcga tcggcgccgt ctccttcggc agggcgaagc
     1981 cgaggcgctt gacggcgagc ccgcccacgc cggtgatcgc gaaggccagc gcgagcttca
     2041 agagatacgc ccgagccgcc ggctcgtgca gtcgccccag caggtcgcgc aggcggcccc
     2101 cgaaggccag caggaccgcg agggccgcgc cggactggat cgtgatgttg aagagatccg
     2161 actggcgcgg cacccatccc gcgttctcca ccaggagcag gtgcccggtc gaggagatcg
     2221 gcaggaactc ggtcacgccc tcgatgatgc ccaggatggt gacggccagc cacgcaggca
     2281 ctggcgggtc agcccacgat ctcttcgccg ccgtcgactc cgatcacgtt gccggtgatc
     2341 cagtgcgtgt cggggtggct cagcacgacg atcgcgcggg ccacatcgtc ggtcgtggtc
     2401 agccggcgat gcggattgcg ccgcgcggcc tgctgggaga actcctcgta gttcgggatc
     2461 ttctgggccg cgggagtcgc ggtcacgccc gcccggatgg agttggcggt gatgccttgc
     2521 ggggccagct cggccgcgag ctgccggatg ttggactcga gcgccgcctt ggccgcggag
     2581 accggcccgt agtagggcag cacgcgcgcg ccgcccgccg aggtcatggc atagatgcgc
     2641 ccgccgcggc tcatgaggcc gcgccccacc agctcctgcg cccagtagac gaggctgtgg
     2701 gccatcacgt cgagcgtcat gtccatctgc gccttggtca ccgcttcctt catcggatcg
     2761 gccacgaaga gcttgagcgt gccgaaggcc agcgaatgca gcatcacccg cacctggccc
     2821 acctggttgc gctcgcggag cacccgctcc atctcggtcg cgacctcggc gcgcttctcc
     2881 tcgtccgccg cgttgacgtt gaagaagtgg gcctcccgcc cgagggactt gatctcggag
     2941 gccaggcgct cgacgttggg cagggtggac ttgcggtcga gatggacgcc gaacacgtcg
     3001 agccccgcgc gcgcgagcgc caggctcgtg gcggcgccga agccggagga ggcgcccagg
     3061 atcagggccc agccgcgcag tgtgatcgga gaggcgtcgg tggtcatggc attcctctgt
     3121 tcgagggagt cgcccgtcgg agcgcgcccc attctatcag aactccccgc atgctacgat
     3181 gagcctcggc catggcgaag acggccggca tcctgctgat cggcaacgag atcctctcgg
     3241 gcaagatcac cgacgccaac gccgcctacc tctgccgcga gctgcggggg ctcggcgtgg
     3301 aggtgcggcg gatcacggtc atccccgacg aggtggacct catcgcggcc gaggtgcgcg
     3361 ccttcagcgc cgcgttcgac gtggtgttca cctcgggcgg cgtcgggccc acccacgacg
     3421 acgtgacgat cgagggcgtg gcgcgcgccc tcggcgtccc ggtgatccgc gagccgcggc
     3481 tggtgcgcct gctcgagggc tactacaagg gccgcctcaa cgacgcgcgc ctcaagatgg
     3541 cggaggtgcc cgaaggggct gagctgaccg cggacggcga gctggtgttc ccggcggtgg
     3601 tggtgcggaa catctacatc ctgcccggcg tgcccgagat cttccgccag aagttcgacg
     3661 cgctcaagga gcgctttcgc gacgcgccgt tccatctctg ctgcgtgtac gtgagcatgg
     3721 gggagggcac gctcgccgat cacctgaacg cgctgctcgc gggccatccg gatctgctcc
     3781 tgggctcgta tcccgagttc tccaacccgg aatacaaggt gaaggtcacg ctggagtcca
     3841 aggaccgcgg ctatctcgac gacgcgatca ccgatttcct gggccgcctt ccccaggacg
     3901 cggtggtccg ggtcgaccgg tgaggccccg gcgcgcccgc gtctgctgac cgtcccgctc
     3961 gccgtcctca tcgtcaccgc cgcgctgtac gtggcgcgtc tcggcgccgc gcccttcctc
     4021 gatcccccgg aagggatcca cgcggagatc gcgtgggaga tgctgcgggg cgcgggctgg
     4081 atcacgccgc atctcaacgg cgtgccgtac ttcgacaagc ccccgctgcc ctactggctc
     4141 atggcgggcg ccttcgcgtg gctcgggccc accgagacgg cggcccggct cgtgtccgcg
     4201 ctcgccacgg tcgcggtggc ggtgctcacc gcgctcatcg gcacccggct ggcctcggga
     4261 cgggtcgggc tcatggcggg gctgatggtc gcggccaacc tcgaggtctt cctcttcggc
     4321 cggatggtca agccggacat gctcctcgtc gcctgcatcc tcctcggcct ctatggcttc
     4381 atcctggcct acctgggcgg cggccggtgg ccgctcctgc tcttctacgg gggcctgggc
     4441 ctggcggtgc tggccaagga cttcctgggc gcggtgggcc cgctcgccat cgtcgcgctc
     4501 ttcctgtggc tcacccgcga gggcgggtcg tggcggcagt gggcgccgct ctccgcgatc
     4561 ctgctcctgc tcgcggtcgc ggtgccctgg tacctgctgg tcgaatcaca gtaccgcggc
     4621 ttcctctggt acacggtcgt ggacaaccac gtactgaacg tcgcgcgcca gcgcgtcttc
     4681 cccgacgagg atgtgccgct gtccacgctc gagttcctgg gcgtcaccgc gctcggcttc
     4741 ttcccctggg tgctcgcgct gccgctcgcg gtcgcccggg tgatccggcg gcggccgtgg
     4801 aagacgcccg aggcgcgggt ctggatgctg ctcgccctct ggacggtcgg catcctcggc
     4861 gtcttcgccc tgtcaccctt caagctgccg cactacggcc tgcccgcgtt tcccgcgatg
     4921 gcactcctgg tggctaagct ctgggacgag gccatcgagc gcccgccggg cgcgccgcgc
     4981 tcgttcagcc tgctcgttcc cgcgctgctc gcgacggccc tcgtgggcac cgcgacgctc
     5041 ggcgcctggc tgggctggct ccgcctctcc ccggaggcgg tcgcggtggc cgacgtctcc
     5101 gcgcggaaca tggccgcgca gggccaggtc gcgacctccg acttcctggc gcagttccgg
     5161 ccgctcttcg gtccgatcgc ggggatcttc ctgctgacga cggcggcgct ggcggtagcg
     5221 gcctggcgcc ggctcccgcg tctgggcctc ggcgccctgc tggccgccat gatcgcgttc
     5281 ctgccgctct ccgtcgaagg tctcacgctc ttcgccaaga gccgctcggt gcggctgatg
     5341 accgacgcga tcctgctccg tgccgggccg ggggacgtgc tcgcccacga gggcgcgatc
     5401 gagaacagcg cctccgggct gctgcgcctg gatcggcggg tgcagatcgt caacgggctc
     5461 cagtccaacc tggccttcgg ctcgacgttt gccgaggcgc ggccgcggtt ctgggacagc
     5521 gcggcgctgg cgcacgcctg ggcgggggac cggcgcgtct tcctgctctc ggccgccacg
     5581 cccagccgga gcgtggtgcg cgagctgccg gccgaccacg ttcatctgct ggtcgagggg
     5641 ggaagccggc ggctctattc caaccggccg tgagcacggc gggtgctatc ctggcccagg
     5701 agaaggagga atggacgccg cgagccagct ccgtcaggcg caccggacca ccgcgctcat
     5761 caccggcggt ctcatcacgg gcctggtgat gtacgccgcg atcgccggct tcgtcctggt
     5821 gatcctgggc gggcccttcc tcgacttcta cctggtcggc tcgcggtcgc tcgcgttgct
     5881 gcacgtcaac gctcctcgct acgagacgtg ggaggcgtgg ctggccacgc cggcgccgcg
     5941 gccctgaccg tgggcgtctc cccgtaccgg ctcaaggacg acccgcactc gagccactcc
     6001 gtcatcctgt cgcgactcgg cgagggccgc ggccgcaagg cgctggacgt gggcgccgcc
     6061 gacggcttcc tcgcggagcg tctcaccgcc cagggctgga gcgtgaccgc gctcgagcgc
     6121 gacccggagc tcgccgcgcg cgcgcgcggg cggtgcaagg aggtggtggt ggccgacctg
     6181 gagtcggcgc cgccgctgct gcacggcccc ttcgaggcca tcgtctacgg cgacgtgctc
     6241 gagcatctga gcgatccggg agcggcgctc cgggcgctcg accagaccct cgcccccgag
     6301 ggtaccgtga tcgtctcggt acccaacgtc gcgcatctct gggtgcgcct gtcgctcctg
     6361 gcgggccgct tcgactacgc cgatcgcggc atcctggatc gcacccacct ccgcttcttc
     6421 acgcggcgca ccttgctcga tcttctgcgc tcggccgggc tcagcgtggt cgagctcgcg
     6481 gtcaccccgg tgccgttgcc gctggtggtg tccccgcgct ggcgcggacg ctggctctcg
     6541 cgcgtgcacg cgctgagcgc gggggcggcg cggggctggc cgggcgggct cgcctaccag
     6601 ttcgtcgcgg tctgccgcgc gaggaccagc tagatggccg gcccgttctc gaagggcgcg
     6661 cccaaggtcg tggtggtgat gcccgcctac aacgcgggcc ggacgctgcg catgacgtac
     6721 gaggagctgc cgaaggacac cgtgaacctg gtcatcctcg tggacgacgg ctcgaccgac
     6781 gccacgctgg aggtcgcacg cgatctcgga ctcgagatct tcgtccacga caagaactac
     6841 ggctacggcg ccaaccagaa gacctgctac accgaggcgc tcaaggcggg cgccgacatc
     6901 gtggtgatgg tccatcccga ctaccagtac gatcccaccc tggtgcccca gattatcgcc
     6961 cccatcgcgc ggggcgaggc cgatctcgtc ctgggctccc ggctcgcggg cggcggctcg
     7021 gcgatcgagc aggggatgcc gtggtggaag tacgtggcca accgtttcct gaccggggtg
     7081 gagaacatgg ccttcggtct ccacctgtcc gagtaccaca ccggctaccg cgcgttcagc
     7141 cgaggcgtgc tcgacgccgt caacttccgc atgaactccg acggcttcgt cttcgaccag
     7201 gagatcgtcg cccaggcggt ggccaccggc ttccggatcg cggagatccc ggtaccggtg
     7261 cgttattttc ccgaggcctc ctcggcgagc ttcagcgcat cctgcgtcta cggcctcaag
     7321 atcctctggg tcgtcgcgcg ctatctgctc caccggacgg ggctcaagcg ctcccgccgg
     7381 ctcggctcca tcgcgggccg ctacagtcgc ctcgggcgtc ccggctcgcc cccaccagcc
     7441 gacagccggt gatcgtcgtc cggcttctcg acggcgcggc cgtcctggcc gcggccgggc
     7501 tgggcctggc gctcgcgttc tggacgggcc ggcgcgagca ctggctctgg aggcccgagc
     7561 acctcttcct cctgctcctg gggctcgtgg cactgcgcgt gtggctggcg cccaggccgc
     7621 tgcccgcggt gcggccccgc cgggccgtcc tggtcggggt ggtggcgtac gcggtcgtgt
     7681 tctccttcgt cacggtcacg cgccacctga ccttcgcgac gcacgcgctc gatctcggct
     7741 actacgtcca gctcgtgtgg aacctggcgc ggggcgccgg cccctacgtg agcctgcccg
     7801 agatgcacgc ctggggcgat cacctctcgc cgatcatgta cgtcttcgtg cccgcctact
     7861 ggctggtccc gggcccggtg gtcctgctgg tcgcccagtc ggtggcgctg gccctgggcg
     7921 ggttcgcggt gttcggcctg gcccgggcgc gactcggcga cgagcgtccc gccgccgcgt
     7981 tcgcgctgct ctacctggtg aacccgtcgc tccacgggat caacgtacgc gacttccacg
     8041 cggccgcgct cgcgatcccg ctcctcctgg ccgccttctg ggccgtggag acgggccggc
     8101 cgtggctggc gctcgcgccc gccgcgctca cgctgctctg ccgggaggac gccgcgttgc
     8161 cggtgatggg gctcggcgcc tggatggcgg cggcccaccg ccgctggctc gcgggcgtcg
     8221 tgaccgcgac cgtcgcgctg gccctgctcg cggtggacgt gcgctggatc atcccggcct
     8281 atcgcggcga gccctactcg catctcggcc gctacgccca cctcggcggc tccctcgccg
     8341 agatcatcgt gaacgcgctc gtccatccgt ggcgcacgct ggccacgctg ggcaccgtgg
     8401 accgcgcggt gtacctggcc gcgatgctgg ccccgctcgc gttcctgccg ttgctgggcg
     8461 gctgggatct gctgggcgcg ctgcccgccc tggcccagaa tctcctcagc cgagacccgg
     8521 tgctctacgg gttccgcacc cagtaccagt ccttcgtcct gcccttcctg atcctggccg
     8581 cgatcggcgg ctacgcgcgc ctcgcgaggc ggcggcccgg cgcctggccg gcggcggtgc
     8641 tggtggtcgc gatggtggtg agcctcgcgc tcgcctcgcg caccgtgaac aacctcgcga
     8701 tcgcccgctt ctggcccgtc ccggagcaac gcgccgccta ccaggtgctg gcgcgggtcc
     8761 ctcccgccgc cgcgatctcc gcgcaggatc cctacgtgcc gcatctcagc ctgcggccgc
     8821 tcgtcttcgt cttcccggtc ggcctcgaga agagcgacta cgtgctcgtg aatctcgaca
     8881 gctacccgtg gcggaacctg ccggacgtga ccctcacgcg ggacgggagt cgcgtgacca
     8941 tcgtggcggg aggcgtcgag cggcgctacg cggtggccgc ccagggcggc ccgcacctgc
     9001 tcctgcggcg cctctagtca ggtcagggga acataatcgt agtcgcccac gccctgcagg
     9061 acgaggaaga ccgcggacgt ctccccgccg ttggtgacca ggtgcgggcg cccggcgcgc
     9121 accgcgtacg aatcaccggg ctcgaggcgg atctcctcct tcggatcgcg gaggaagaga
     9181 cgcagccgcc cctccaggac gtagaaggtg tcccggatgc tcgtgtggta gtgccacggt
     9241 acctgctggc gcgggccgat ctgcaactcg gtgatgcgaa agccgggccg ctcggcgtgg
     9301 cgcgcgcgac gctcgacctc gtagagatgg ctcgcgtcct tcacgggctc catgcgtgct
     9361 cctcccgggg tggtcacgcc cccgccgacg ggtcgccggt gaggatgtgg gaagcgtggt
     9421 ggtggatgag gagccactcc ccgccgcttc gctcgaagat gttggtggcc aggaccgacg
     9481 tgaccgagat gttgccgcgg gactcggaca ggatgttctc ggtgcagctg acccacccga
     9541 agccgcccgc cacgtgcgcg tgaacgtcgg tgatgctgaa gcgcatctcc tggctgttct
     9601 tgaagatgac ctcccaggag tggcgcaccg cctcccagcc cgcgagcatg caccagcccg
     9661 gatgcacgca ccgcacgtgg tcctcgtggg cccagatctc gtccatctgg ttgagatcga
     9721 gggtctcgaa gacgcggtag aagcgcgcgt tcgcctccgc gaggtccgcc cgctcgtggt
     9781 ccgccgtcat gggcccaggt tctatcagat cgcccgcgcg tcggcaaacg cgcggcccgc
     9841 gtccggaatc gcctccagac cccgtggtag gatgagcggg cgatgccggc ctcacccctc
     9901 gcagtcgaag cgcgcgatct cgtcaagacc ttccgcagcg gatggctccg ccgccgggag
     9961 acggccgccc tgcgcggagc gagtctccag gtgccgcgcg gcgccatcta cggcctgctc
    10021 ggcccgaacg gagcgggcaa gaccacgctg ctctccatcc tggccacgct gctgctgccg
    10081 gacagcggct ccgccaggat cctgggccac gacgtcgtcc gcgaggcctt cgcgatccgg
    10141 cgccgcctca acatggcgag cggcaacgcg tcgttcgtct ggagcctgcg cccggccgag
    10201 gtgctcgcct tctacgggcg cctctacggc ctccacgggc gcgcactcaa gtgccgcgtg
    10261 tacgagctgg tcgagcgctt cgagctgacc ccgcacctca agacggagta caacgagctc
    10321 tccaccggcc tcaagcagcg gctggccctg gccaaggcgc tcctgaacga tccggaggtg
    10381 ctcttcctcg acgagccgac gctcggcctc gatccggacg tgtccatccg catccgggcc
    10441 cagatcgccg agctgcggcg ggagcgggac accaccatca tcctgaccac ccactacatg
    10501 cgggaggccg aggagctctg cgaccgcatc gccttcatca agggcgggcg catcctggcc
    10561 gaagggtcgg cggacgagct caagcggcag atccgcatcg gggacgtgat cgcgctccgg
    10621 ctcgagcccg cggttcccgt cggactcgcc gagctgccgg gcgtgatggg cgtgcaggcc
    10681 cgcggcgatc ggatcgactg cacggtggat tcctcggaga agcggctgcc cgacatcctc
    10741 aagtggctct gcgaccacgg ggtgctcatc cgcgactgcc aggtgcgcga gcccgatctc
    10801 gaggaggtct tcgttgagct ctcgcgctga gcccctcccg acgaccgtcg cggaatcctg
    10861 ggcggcggag tgggcgcggc tgaccgcctt cgcctatcgc aacctgatca tggcgcggcg
    10921 caacgtcttc ttcgtcttcg agctcacgtt ctggccgggc gtggccatgc tctcgcacgg
    10981 cctgctcacg cgcttcctcg acctgacccc cgagatgacc gcgttcatcc tggtgggcac
    11041 cgtggcgctc tccgcggtgc aggtctgcca gctcgacgtc gcctacgcgg tgctcttcga
    11101 catctggtcc aagtccatga agcaccagtt cctgactccg gtgcgcatcc accacctggc
    11161 gctcggctcc tggctggtcg gggtagggcg gggcgtgatc gtgtgggccc tcatggcggt
    11221 gatcggcggc cgcgccttcg gcttcgactt cctcgggccg ggaccgctgc gggtcgcgct
    11281 cttcctgctc ggctgcttcc tcaccgcgct cgtgatcggg ctcctcgtct gcgcgctggt
    11341 gctcctctac ggcacccgcg ccgagacctc cgcgtgggcg gcggtgaact tcttcgtgat
    11401 gctcggcggg atctactacc cgatctcggt gctgccgggc tgggtgcagg cgatctccgc
    11461 ggggatcccc ctgacctact ttctcgacgg gatccgggtc ggctacggct tcccgtccca
    11521 gttcgcgtat cccttcgcga ccggcttcgc gctctcgctg ctctacgcgg tcctcgccca
    11581 ctgggcgctc gccgccgcgg tcactcgatc ccgccggacc ggcctcctgc tcaagctgtc
    11641 ggagtgaggc catgaagcag ggcgccgcct cgatcaagct
//
LOCUS       DASUFA010000075         7361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1384747, whole genome shotgun sequence.
ACCESSION   DASUFA010000075 DASUFA010000000
VERSION     DASUFA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384747"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..941
                     /locus_tag="VFX87_02975"
     CDS             198..941
                     /locus_tag="VFX87_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX4993898.1"
                     /translation="MSTLHEDDLRRRILARPIPRHVAVIMDGNGRWATSRGLPRVAGH
                     GEGVKSVRAVVRTAGELGIEFLTLYAFSSENWNRPHQEVSTLMTLLERSIERELPELM
                     ERNVRFRVIGRAAGVPARVGERIQRLIETTSRNTGLTLLMAFNYGGRDELVDAVRALA
                     REVQSGRLRPEDIDEVRVSRALYTDGIPDPDLLIRTSGELRLSNFLLWQVAYTELWMT
                     PTLWPDFGAREFCLAVADFQQRDRRFGRV"
     gene            941..1810
                     /locus_tag="VFX87_02980"
     CDS             941..1810
                     /locus_tag="VFX87_02980"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX4993899.1"
                     /translation="MARAQGVPLADSVEQTVPSHGGGLGRRLLSTLILLPLFVWMVAS
                     GPVWLFGAVMVLAGALGQWEFTGMFERAGARPFRVLGLLGGSLVTASFALPVSERVAF
                     TAVLLALLAVGLLRRQVGRPIWEPVAVTLLGVCYVNWLLGYTFWLRDLDAGREWVLLL
                     VSVTWLGETAAYLVGSRLGRHKLAPSISPRKTVEGALAQLVMSVLAALGARATFFPAL
                     SLEGAVVVGLLLGVVGQAGDLLESAIKRSVGTKDTGRLIPGHGGMLDRVDSLLINTPV
                     LFYYATYARSLGA"
     gene            1807..2982
                     /locus_tag="VFX87_02985"
     CDS             1807..2982
                     /locus_tag="VFX87_02985"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX4993900.1"
                     /translation="MKRLIVLGVTGSVGRRTLELAEHFPDDFRVEGMAARGSNPELLA
                     ELCRRHRPRALALAEASAIDTVARALGSPCPELLAGPAGIVTLAGEVEADVVLSAIVG
                     GAGLLPTMAAIKSGKSIALANKETLVMAGSLMTAAARERGVPLLPVDSEHSAVFQCLE
                     GHNRAEVGRVLLTASGGPFRTMKAADLAKVTVEEALNHPTWRMGAKITVDSATLMNKG
                     LEVIEARWLFDLRPEQVQVVVHPQSIVHSMVEYVDGSVIAQLGVADMGIPILYALTYP
                     QRLPCPAERLDLTRVGPLTFEEPDVQRFPCLDLARRALLEGGCAPVILNAANEIAVAA
                     FLDGRIRFTQIPELIADALAQVPARALDSIDTCVDVDTRTRAVVRDWLPGGVAAAMR"
     gene            2991..4352
                     /gene="rseP"
                     /locus_tag="VFX87_02990"
     CDS             2991..4352
                     /gene="rseP"
                     /locus_tag="VFX87_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX4993901.1"
                     /translation="MATVQMLVSFVVVLGILIIVHELGHFLMARLCGVGVERFSIGFG
                     PVLWRFRGKETEYCLSLIPMGGYVKMMGDDENPLEGGKAATVDPARAFNNKPLAARFL
                     IVFAGPAMNFVLAVLIAAAMFTLVGRPVAPAEVGRVTEGGPAAQAGLRTGDRVVAVDG
                     KPVQYWEDLARVVQAASGRPLEVTVQGSDAASRKVALTPAQAKRKDLFGDEQTVWEIG
                     ASPYLAPAIGDVIPGDPAAQAGLKAGDVVVALEGQPVMSWDELAEKIHQRAGQPTRLE
                     AKRGTETVTITVTPKKGKVPGPDGKEMEVGLVGIRPGGATMMVRSNPVSAMWEGLAWS
                     TDVTVKTGIGLYKIVVGQLDRSNIGGPIQIAKTAGEQARQGLVSLALFTAVISINLFL
                     LNLLPVPMLDGGHLLFFAFEAVLGRPLSVRKREVAQQVGFALLMLLMVFAFYNDFKRI
                     GLF"
     gene            4352..4675
                     /locus_tag="VFX87_02995"
     CDS             4352..4675
                     /locus_tag="VFX87_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX4993902.1"
                     /translation="MGQYERHVFVCTVGDSCPVQGDVEKYVKTLRAGVVTAGRQVEVR
                     VNKAGCFSQCGHGPMIVVYPENVWYAGVRESDLPEILESHILNGVPVKRLIYDPGVKG
                     ANKVK"
     gene            4683..6377
                     /locus_tag="VFX87_03000"
     CDS             4683..6377
                     /locus_tag="VFX87_03000"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX4993903.1"
                     /translation="MRWTRSLIPTLRDDPADAEAISHKLMVRAGLVRQLAAGIYVYLP
                     LGLRVIEKVNAIIREEMNGIGGQEIEMPVLHPAELWQQSGRWDVIGGEMFRLKDRNGR
                     DMCLGMTHEEVIAWLAAREIRSYRDLPQIWYQIQTKERDEARPRSGVLRTREFLMKDS
                     YTLDPDAAALEISYNAHKDAYCRIFERCGITYVVVQSDPGMMGGSGSHEFMAPSAAGE
                     DDVALCDACGYAANVELARGVPSAPPNSDAKMEEVATPGVRTIAEVSARLKIDPAATI
                     KSLVFMGPAGPVLALVRGDHALHERKLARALQAEARPAHPEEVKQHLGVAPGSIGPVG
                     VRGARIIADESLRRGRYVVGANRDGFHLTGVTPGQDFACEWADLQVALAGEGCPSCGK
                     PLRIERVIEVGNIFKLGTRYSVPLGASYLDERGQQQPIVMGSYGIGPARIAAAAVEQR
                     HDADGIVWPWAIAPFHVHLVPVAVKDVAQMAAAEEIYRELRAAGFDVLMDDREERAGV
                     KFKDADLLGIPIRVTVGNALAKEGVVELRQRSTRADRRVPKADVVATIREMAQAIS"
     gene            6464..6949
                     /gene="rimP"
                     /locus_tag="VFX87_03005"
     CDS             6464..6949
                     /gene="rimP"
                     /locus_tag="VFX87_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX4993904.1"
                     /translation="MAEDAQWLDQIEAVVAPVLASRGMSLVDSEWRREGRRWVLRLFV
                     DKPGGVGIADCQAFSREAGDVLDVSGLIEPSYDLEVSSPGLDRVLKKDRELAWAIGRD
                     VHCWVAEAVDGRTEFSGRLLEVSPGALTLEETVGTRREIPRRLVTKARLEVVAFGRAK
                     R"
     gene            6946..>7361
                     /locus_tag="VFX87_03010"
     CDS             6946..>7361
                     /locus_tag="VFX87_03010"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX4993905.1"
                     /translation="MMGKDLIYVIEQIGREKGISKEVLFEALESALLSASKKTMGAAD
                     NVRMEIDRRTGTLRVFARKKVVTAVTDPKLEISVAEGRKLNGEAQLDDELEIELAPQE
                     FGRIAAQTAKQVILQRVRDAERDAVYSEFIDREGKIV"
BASE COUNT         1055 a         2458 c         2709 g         1139 t
ORIGIN      
        1 tgaaatgcgg gggcccagaa atggcccccg cactccccca gtccgcggac atccgttgtt
       61 gtttcgtggc cgcaattccg cggaatccca gtcccttttc cgcggtttag ggggtttttc
      121 ctggcaattg ctggggggct cacgtaacat ggcctgatat ggcggcgaag gccgggatct
      181 ccgcggcggt gtcagagctg tcgacgctcc acgaggacga tctcaggcga cgcatcctgg
      241 cccggccgat cccgcggcac gtggcggtca tcatggacgg caacgggcgg tgggccacca
      301 gccgcgggct gccgcgcgtg gccggccacg gcgagggcgt gaagtcggtg cgggcggtcg
      361 tgcgcaccgc gggtgagctc ggtatcgagt tcctgaccct ctacgcgttc tcctccgaga
      421 actggaatcg cccccaccag gaagtctcca cgctgatgac cctgctggag cgctcgatcg
      481 agcgcgagct gcccgagctc atggagcgca acgtgcggtt ccgcgtgatc ggccgcgcgg
      541 ccggggtgcc cgcgcgggtg ggcgagcgca tccagcggct catcgagacg acgtcgcgca
      601 acaccggcct caccctgctc atggccttca actacggcgg ccgcgacgag ctggtggacg
      661 cggtgcgcgc gctcgcccgc gaggtacaga gcggacggtt gcggccggag gatatcgacg
      721 aggtccgcgt ctcccgcgcg ctctacaccg acggcatccc cgatccggac ctgctgatcc
      781 gcacgagcgg ggagctgcgc ctgtccaact ttctcctgtg gcaggtcgcc tacaccgagc
      841 tgtggatgac gccgactctc tggcccgatt tcggcgcgcg cgagttctgt ctcgcggtcg
      901 ccgacttcca gcagcgggac cgccgcttcg gacgggtctg atggcgcgcg cgcagggcgt
      961 gccgctcgcc gactcggtgg agcagacggt cccgtcccac ggcggagggc tcgggcgccg
     1021 gctcctgagc acgctcatcc tgttgcccct gttcgtgtgg atggtggcga gcgggccggt
     1081 gtggctcttc ggggcggtca tggtgctcgc gggcgcgctc ggccagtggg agttcaccgg
     1141 aatgttcgag cgggcgggcg cgcggccctt ccgcgtgctc gggctgctcg gcggctcgct
     1201 cgtgaccgcg agcttcgcgc tgccggtatc cgaacgggtg gccttcaccg cggtcctcct
     1261 ggccctgctc gcggtcgggc tgctgcggcg ccaggtgggc cgcccgatct gggagccggt
     1321 ggcggtcacc ctcctcggcg tctgctacgt gaactggctt ctcggctaca ccttctggct
     1381 gcgcgatctg gacgcgggcc gggaatgggt cctgctcctg gtctcggtca cgtggctcgg
     1441 ggagaccgcc gcctacctgg tgggctcccg cctgggccgc cacaagctgg cgccgtcgat
     1501 cagcccccgc aagacggtgg agggcgccct cgcacagctc gtgatgtcgg tcctcgccgc
     1561 gctgggcgcg cgggcgacgt tcttcccggc gctctcgctc gagggcgcgg tcgtggtcgg
     1621 gctcctgctc ggggtggtgg gccaggccgg cgatctcctc gagtccgcca tcaagcggag
     1681 cgtcggcacc aaggacaccg ggcggctcat ccccggacac ggcgggatgc tcgaccgcgt
     1741 ggacagcctg ctcatcaaca cgccggtcct cttctactac gcgacctacg cgaggtccct
     1801 gggcgcatga agcggctgat cgtgctggga gtgaccggtt ccgtggggcg gcgcacgctc
     1861 gagctggccg agcatttccc cgacgacttc cgggtggagg gcatggcggc ccgcggctcc
     1921 aatccggagc tcctcgcgga gctctgccgc cgtcaccggc cgcgggccct ggccctggcc
     1981 gaggcgtcgg cgatcgacac ggtggcgcgc gcgctggggt ccccgtgccc cgagctgctc
     2041 gccgggcccg cgggcatcgt gacgctcgcc ggagaggtcg aggccgacgt cgtgctctcc
     2101 gcgatcgtgg gcggggcggg tctcctgccc accatggccg cgatcaagag cggcaagagc
     2161 atcgcgctcg ccaacaagga aacgctggtg atggcgggca gcctgatgac cgcggcggcc
     2221 cgcgagcgcg gcgtgccgct gctgccggtg gattccgagc acagcgcggt cttccagtgc
     2281 ctcgagggcc acaaccgcgc cgaggtcggg cgggtgctgc tcaccgcctc cggcggaccg
     2341 ttccggacga tgaaggcggc ggacctcgcg aaggtcacgg tggaggaggc gctgaaccat
     2401 ccgacgtggc ggatgggcgc caagatcacg gtggactcgg ccacgctcat gaacaagggc
     2461 ctcgaggtca tcgaggcgcg ctggctcttc gacctccggc ccgagcaggt gcaggtggtg
     2521 gtgcacccgc agtccatcgt gcactcgatg gtcgagtacg tggacgggtc ggtcatcgcc
     2581 cagctcggcg tggccgacat gggtatcccg atcctctacg cgctcacgta cccgcagcgc
     2641 ctgccctgtc cggccgagcg cctggatctc acccgggtcg ggccgctcac cttcgaggag
     2701 ccggacgtgc agcgcttccc gtgcctggac ctggcgcgcc gggccttgct cgaaggcggg
     2761 tgcgcgccgg tgatcctcaa cgcggccaac gagatcgcgg tcgccgcgtt cctcgacggc
     2821 cgcatccgct tcactcagat tcccgagctg atcgcggacg cgctggcgca ggtgcccgcg
     2881 cgggcgctcg acagtatcga cacgtgcgtg gacgttgaca cccggacgcg ggcggtggtg
     2941 cgggactggc tgcccggcgg ggtcgcggcc gcgatgagat agggagcctc atggcgacgg
     3001 tgcagatgct cgtctcgttc gtggtggtgc tcggcatcct catcatcgtg cacgagctcg
     3061 gccatttcct catggcccgg ctctgcgggg tgggggtcga gcggttctcg atcggtttcg
     3121 ggcccgtgct ctggcgcttc cggggcaagg aaaccgagta ctgcctgtcc ctgatcccga
     3181 tgggcggcta cgtaaagatg atgggagacg acgagaaccc gctggagggc ggcaaggcgg
     3241 ccacggtgga cccggccagg gccttcaaca acaagccgct cgcggcccgg ttcctcatcg
     3301 tcttcgcggg ccccgcgatg aacttcgtcc tggccgttct catcgccgcc gcgatgttca
     3361 cgctcgtcgg gcggccggtg gcgcccgccg aggtggggcg tgtgaccgag ggcgggcccg
     3421 ccgctcaggc ggggctccgc actggcgacc gcgtcgtcgc ggtggacggc aagccggtgc
     3481 agtactggga ggatctggcg cgcgtggtcc aggccgccag cggtcgcccc ctcgaggtga
     3541 cggtgcaggg gagcgacgcc gcctcccgca aggtcgccct gacccccgcg caggccaagc
     3601 gcaaggatct cttcggcgac gagcagaccg tgtgggagat cggcgcctcg ccgtacctgg
     3661 cgcccgccat cggcgacgtc atccccggcg atcccgccgc gcaggcgggg ctcaaggcgg
     3721 gcgacgtggt ggtcgcgctc gaggggcagc ccgtcatgtc gtgggacgag ctggccgaga
     3781 agatccacca gcgcgcgggg cagccgaccc ggctcgaggc caagcgcggg accgagacgg
     3841 tgacgatcac ggtgacgccc aagaagggca aggtcccggg ccccgacggc aaggagatgg
     3901 aggtcggcct cgtggggatc cgcccgggcg gggccaccat gatggtccgc tcgaatccgg
     3961 tctccgcgat gtgggaaggg ctcgcgtggt ccaccgacgt cacggtgaag acgggcatcg
     4021 gcctctacaa gatcgtggtc ggccagctcg accggagcaa tatcggcggc ccgatccaga
     4081 tcgccaagac cgcgggcgag caggcgcgcc agggcctcgt gagcctggcc ctcttcaccg
     4141 cggtcatcag catcaacctc ttcctgctca acctgctgcc ggtgcccatg ctcgacggcg
     4201 gccacctcct cttcttcgcc ttcgaggcgg tgctcggccg gccgctctcg gtgcgcaagc
     4261 gcgaggtcgc ccagcaggtc gggttcgcgc tcctcatgct gctcatggtg ttcgcgttct
     4321 acaacgactt caagcggatc ggactcttct gatgggacag tacgagcggc acgtcttcgt
     4381 gtgcacggtg ggcgatagct gcccggtcca gggcgacgtc gagaagtacg tcaagacgct
     4441 gcgggcgggc gtggtcacgg ccggccgtca ggtcgaggtt cgcgtgaaca aggcgggctg
     4501 cttctcgcag tgcggccacg gcccgatgat cgtggtgtac ccggagaacg tctggtacgc
     4561 gggcgtccgg gagagcgacc tccccgagat cctcgagtcc cacatcctga acggggtgcc
     4621 ggtcaagcgc ctgatctacg atccgggcgt caagggcgcc aacaaggtca agtaggcggg
     4681 ccatgcgctg gacccggtcg ctgatcccca cgctccggga cgatcccgcc gacgccgaag
     4741 ccatcagcca caagctcatg gtgcgcgccg gcctcgtccg gcagctcgcc gcgggcatct
     4801 acgtctacct cccgctcggc ctgcgcgtga tcgagaaggt caacgcgatc atccgcgagg
     4861 agatgaacgg catcggcggg caggagatcg agatgccggt gctgcatccc gccgagctct
     4921 ggcagcagtc gggccggtgg gacgtgatcg gcggggagat gttccgtctc aaggaccgca
     4981 acgggcgcga catgtgcctc gggatgaccc acgaggaggt catcgcctgg ctcgccgccc
     5041 gcgagatccg ctcctaccgc gacctgccgc agatctggta ccagatccag acgaaggagc
     5101 gggacgaggc gcggccccgc tccggggtgc tgcgtacgcg cgagttcctg atgaaggact
     5161 cctacacgct cgacccggac gcggccgcgc tcgagatctc ctacaacgcc cacaaggacg
     5221 cctactgccg catcttcgag cgctgcggga tcacctacgt ggtggtccag tcggatcccg
     5281 gcatgatggg cggctcgggc tcccatgagt tcatggcccc gagcgcggcc ggcgaggacg
     5341 acgtggccct ctgcgacgcc tgcggctacg cggccaacgt ggagctggcg cgcggggtgc
     5401 cgagcgcgcc gcccaactca gacgccaaga tggaggaagt ggcgacgccg ggggtgcgca
     5461 ccatcgccga ggtgtcggcc cggctcaaga tcgatccggc cgccaccatc aagtcgctgg
     5521 tcttcatggg gccggccggc ccggtgctgg cgctggtgcg gggcgatcac gcgctccacg
     5581 agcgcaagct cgcccgtgcc ctccaggcgg aggcccggcc cgcgcacccg gaagaggtca
     5641 agcagcacct cggcgtggct ccgggctcga tcgggcccgt gggcgtccgc ggcgcgcgga
     5701 tcatcgcgga cgagtcgctg cgccggggcc gttacgtggt cggcgccaac cgcgacggct
     5761 tccacctgac cggggtgacg ccggggcagg acttcgcgtg cgagtgggcg gacctgcagg
     5821 tggccctggc cggggagggg tgccccagct gcggcaagcc cctccggatc gagcgagtga
     5881 tcgaggtggg caatatcttc aagctcggga ccaggtactc ggtcccgctg ggggccagct
     5941 acctggacga gcgcggccag cagcagccga tcgtcatggg ctcctacggc atcgggccgg
     6001 cgcggatcgc ggcggcggcg gtggagcagc ggcacgacgc cgacggcatc gtctggccgt
     6061 gggcgatcgc gcccttccac gtgcacctgg tcccggtcgc ggtcaaggat gtcgcccaga
     6121 tggcggcggc cgaggagatc taccgggagc tccgggccgc cggcttcgac gtgctcatgg
     6181 acgaccggga ggagcgggcc ggcgtcaagt tcaaggatgc cgacctgctc gggatcccca
     6241 tccgagtcac cgtcggcaat gctctcgcca aggagggtgt ggtcgagctg cgccagcgga
     6301 gcacccgggc cgaccgccgg gtgcccaagg ccgacgtggt cgcgacgatc cgggagatgg
     6361 cgcaagcgat ttcataggtc cccgccttgc gggggcccaa tccctgtgct aaagtcctga
     6421 aaaaagtggg ccgtcgccca cttttttttg tgggggtggc ggcatggcgg aagatgcaca
     6481 gtggctcgac cagatcgagg cggtcgtggc gcctgtgctg gcctcgcgcg gcatgagcct
     6541 ggtcgacagc gagtggcgcc gtgaaggccg gcgctgggtg ctgcgcctct tcgtggacaa
     6601 gccaggcggc gtgggcatcg ccgattgcca ggccttcagc cgggaggccg gcgacgtgct
     6661 ggatgtgtcc ggactcatcg agccctccta cgatctcgaa gtgtcctcgc cggggctgga
     6721 ccgggtactg aagaaggacc gtgagctggc ctgggcgatc ggccgggatg tccactgctg
     6781 ggtggccgag gccgtagatg ggcggacgga gttctcgggc aggcttctgg aggtgtcccc
     6841 gggggcgctg accttggagg aaacggtggg gacgcggcgc gagatcccgc gccggctggt
     6901 gacgaaggca aggctggaag tcgtggcgtt cgggcgggcc aagcgatgat gggcaaagac
     6961 ctcatatacg tgatcgagca gatcgggcgc gagaagggga tcagtaaaga ggtcctcttc
     7021 gaggcgctgg agtctgccct cctctcggcc tccaagaaga cgatgggcgc ggccgataac
     7081 gtgcggatgg agatcgatcg ccgcaccggt acgctccggg tcttcgcgcg caagaaggtg
     7141 gtgaccgcgg tcaccgaccc gaagctcgag atctccgtcg cggaaggccg caagctgaac
     7201 ggtgaggcgc agctggacga cgagctggag atcgagctgg cgccccagga gttcgggcgc
     7261 atcgcggccc agaccgccaa gcaggtcatc ctgcagcgcg tgcgcgacgc cgagcgcgac
     7321 gcggtgtact cggagttcat cgaccgcgag ggcaagatcg t
//
LOCUS       DASUFA010000076         9333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_132079, whole genome shotgun sequence.
ACCESSION   DASUFA010000076 DASUFA010000000
VERSION     DASUFA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132079"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFX87_03015"
     CDS             complement(<1..757)
                     /locus_tag="VFX87_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4993906.1"
                     /translation="MGPHPARAAVAGLLLLLLFALSAGGCGERPVASGPITLVFKHAK
                     ILGPADPVPGLLRRFEADHPGVRVQSEALTWSSDEQHQFYVINLEGGSPPFDVMMLDV
                     IWVPEFARAGWILDLTPAVAPAERDAHFPTAIAPAMQDGRLWALPWFMNVGLLYYRVD
                     LLAKYGFRPPETYDELTEQVRRIRAGERDPKLDGYLWQGKQYEGGMVNVLEALWANGA
                     RLLDEAGRPFPEPERARESLALLRGLIESGVSPP"
     gene            complement(774..1568)
                     /locus_tag="VFX87_03020"
     CDS             complement(774..1568)
                     /locus_tag="VFX87_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4993907.1"
                     /translation="MRRNRLRELLRAGRPTLGTHIHSAWPAVVELAGHAGVFDYVEFA
                     GEYAPYDLFALENLGRAVNTFDHMSAMFKVEQQPRTYLAVRAIGSGIQNVLFADPRTP
                     EDVRECVRSVRAETPEAGGIHGVGMRRDVGYVMDVGSPAFVQALDDAVVAVMIEKASA
                     VENLEAMLSVKGVDMVQFGPADYSMSIGLPGQWSHPRVVEAERHVIKTALRLGIAPRA
                     EISHPSEAKAYLDLGVRHFCMGWDVSVLFDYFKTEGGALRGMLGAG"
     gene            1626..2834
                     /locus_tag="VFX87_03025"
     CDS             1626..2834
                     /locus_tag="VFX87_03025"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4993908.1"
                     /translation="MRDAPGPLSDLVVLDLTRVLAGPYCTRLLADLGARVIKIERPGE
                     GDDMRKNHLQLEPGRADQSTYFIRINVGKESVAVDLGHPQGRGVVADLCRVADVFVEN
                     FAPGVVEKLGLGYPAMAAIKPDLVYCSISGFGQTGPWRERQAFAHIINAASGMMHLEQ
                     GDQPAPRASNLQAADVLAGTHAFGAILAALRRRDRTGQGAYLDVSMLEALIAADDVSY
                     AAVLNGGEAQGSPRPAMGVFAVGGRHLALQTGGAAALWPRLQALLSRPDLATDPRFAT
                     PDLRRRNWSALRDIIGEWLAGFATVDEALEALRGARIPAAAVLMPEEVVAHPHLRARG
                     AFPTVPHPARGDVRVTASPFQLDGRPVTPAGPAPYRVGEHTRSVLTELLGYKEREIDD
                     LAQAGAVQAP"
     gene            2860..4497
                     /locus_tag="VFX87_03030"
     CDS             2860..4497
                     /locus_tag="VFX87_03030"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4993909.1"
                     /translation="MAATPMTGGEALVRSLYTEGVRVIFGLPGVQLYGVMAALRDEPR
                     IRFITTRHEQATSFMADGYARAGGGFGTALVVPGPGLLNAMSGLSTAYSASSPVLILA
                     GQVPRDSIGKDIGLLHEVNDQLDCITPVTKWRKRVLQVADIPAAVREAVIQLRSGRPR
                     PVELEMPPETMEDEGLAELLPPTEVPRPAAAARDVDRAAELLLGARAPLIYAGGGVHL
                     SGAHDALAAVAEHLQAGVAQSAEGKGAVSDHNALSLGAAYWRDSPLRNHVQEADVVLA
                     VGTRLALVTFKPGTRIVQIDADEREIGRNHKDTLGLAGDARATLEALLERLRAASPPR
                     PSRKAEHEALRARIAADNTMEPNASIIKSLRASTPENAILVAGMTQIGYYSRPFWPVY
                     EPRTYLSSSYSGNLGYAYPVALGAKVARPDRPVVSVSGDGGFLFNAQELSTAARYGIN
                     VVAVVFNDSSYGNVARDLDESWGGQYGADLKNPDFMKLADAYGVLGLRAKEPTEVGRL
                     VREAIDRDRPALVEVPVGRMNRPMFFAPRRTPTKHPR"
     gene            4525..5607
                     /locus_tag="VFX87_03035"
     CDS             4525..5607
                     /locus_tag="VFX87_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEX4993910.1"
                     /translation="MAAAAVERGSAERLRFLDAVRGFALILMILNHTGRWWQDGAMGW
                     PRYNLIYASMAVGAPIFLFLVGFCLPLSFAPRRQAGAPVGAGVVAKFLQRGAKLILAG
                     WALNLFVFRDEPFWEGGVLQTIGLGIILALPPMLVLHRRAARYGLAAFALAFYALFSV
                     LHQEVTAWLPAHRVLGRIFFFEFAPWPWIAIVWIGLALGWVFALQDTAERRARYLTWM
                     TAAGLVCLAVFAIWDWAHGTPIHLSLAFKRDFIVNNHWIARGMTSFLCVGAVFCLLGL
                     AYYAVEVKRLPAGWLVILGRTALMLYFLHHLIVLTLAKELLGLKLNNWWWYALANAVL
                     LVLLVGIGRLWLGIRGFTARRRLQPA"
     gene            5729..6121
                     /locus_tag="VFX87_03040"
     CDS             5729..6121
                     /locus_tag="VFX87_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993911.1"
                     /translation="MSAPAARPKILVVDDEPIIAQLITDVLTGEGYDVDTASDGLVAL
                     EYIGRHGYDLILSDLRMPGLDGLGLYRELDQSRPELARRFVFITGTSEHTDYAGLIGD
                     LAVPVLTKPFDMSALQRVARETLAASVS"
     gene            6306..6977
                     /locus_tag="VFX87_03045"
     CDS             6306..6977
                     /locus_tag="VFX87_03045"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4993912.1"
                     /translation="MQKALAMWLLLAASGGAQPEVSPTQVVQTATEQVLQLVQDGQLA
                     APVSQDQRRIEVQRIADRLFDFQEMSRRSLALHWRERTPQEQAEFVAVFKQLLGRAYI
                     GRLENYSGEQIVYLSEHVDGEFATVRSKIVTGRGAEVPVDYRLHQVGARWVVYDVAVS
                     GVSFVANYRGQFDKIIRGSSFAALMRDLKGKFAETTGRSAVPAVAPAVAPVIAPAVAP
                     APKNP"
     gene            7246..7527
                     /locus_tag="VFX87_03050"
     CDS             7246..7527
                     /locus_tag="VFX87_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993913.1"
                     /translation="MTRAWAGLLVWVLAAGLLVGCGRSKEDLVQKSRGVGTRAELEQA
                     LGKPSDISKLGPVEQWTYKASNGNVVFVIVGDKVTMQATTTPDEKKDQK"
     gene            7539..8378
                     /locus_tag="VFX87_03055"
     CDS             7539..8378
                     /locus_tag="VFX87_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993914.1"
                     /translation="MSESLSDHLTQRLDRLERENRRLKLLGGLALLGLAALTVMGQTA
                     PTSVANTLEAERFVLRDNAGNVRATLGLRPDGTAALALADDTQQDRAVLSVTALGQAG
                     VDLSDRAGRLRAGLGVRPDGSALLTLLDQTDRPRVELRTYGDGRPSLALLDQGGRPRS
                     LLALTAEGATGLTLYDRNGRRRSSMGTEPAGAPAVWLYDTEGNGRAMLAVTGDGQPTL
                     NLTDAGGKGRLWLRVSADFQLPFLSLHDREGRQRVAMLLQQGGVPRLALGDASERVIW
                     KAP"
     gene            8385..9023
                     /locus_tag="VFX87_03060"
     CDS             8385..9023
                     /locus_tag="VFX87_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993915.1"
                     /translation="MPGTNALVLAGLVVYLAASLLLWRLLLKESPPMGRRRSRPRWPR
                     RLAKAFATLLGGIAAGAASLLVVATVVNAGKPGTLTQNEMRAVWAVASVIAMLFIGRA
                     PLIRRVVTRACLAVGVQGVVLPLATLLSFVVAGTRLAGSAGTNGERTVEVFGVRLVGN
                     LPAVWIGLGGFCIGLALVIVGDRAHRQVAKRRARRRFRSSRAGVISARRRHR"
     gene            complement(9076..>9333)
                     /locus_tag="VFX87_03065"
     CDS             complement(9076..>9333)
                     /locus_tag="VFX87_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993916.1"
                     /translation="ARRDEGGVQATVKGRLALWAVAAVLPGPETLRQEVARLESLRGQ
                     IEGLARRLSLQHRRDVYHCRLHVALVRNEARDRRQRTERAR"
BASE COUNT         1265 a         3491 c         3207 g         1370 t
ORIGIN      
        1 agggcgggct cacgccgctc tcgatgaggc cgcgcagcag ggcgagcgac tcgcgcgcgc
       61 gctcgggctc ggggaacggc cgccccgctt cgtcgaggag ccgcgccccg ttggcccaga
      121 gcgcctccag cacgttgacc atgccgccct cgtactgctt gccctgccag agatacccgt
      181 cgagcttcgg atcgcgctcc cccgcccgga tccgccgcac ctgctcggtc agctcgtcgt
      241 acgtctcggg cgggcggaag ccgtacttcg ccagcaggtc gacgcggtag tagagcaggc
      301 ccacgttcat gaaccacggc agcgcccaga gccggccgtc ctgcatcgcg ggcgcgatcg
      361 cggtcgggaa atgggcgtcg cgctcggccg gggccaccgc cggggtgaga tccaggatcc
      421 agcccgcgcg cgcgaactcg ggcacccaga tcacgtcgag catcatcacg tcgaaaggcg
      481 gactgccccc ttcgaggttg atgacgtaga actggtgctg ctcgtccgag ctccaggtga
      541 gcgcctcgct ctgcacgcgc acgcccgggt gatccgcctc gaagcgccgg agcagcccgg
      601 gcaccggatc ggcgggcccc aggatcttgg cgtgcttgaa gaccagcgtg atcggcccgc
      661 tcgcgaccgg gcgctcgccg cagccgcctg ccgacagggc gaagaggagc agcaggagga
      721 ggccggcgac cgcggcgcgc gccggatggg gccccatggg cagccgccgc ggcctagccg
      781 gcgccgagca tgccgcgcag ggcgccgccc tcggtcttga aatagtcgaa cagcacgctc
      841 acgtcccagc ccatgcagaa gtgccggacg cccaggtcca gataggcctt ggcctcgctc
      901 ggatgcgaga tctcggcccg cggggcgatg cccagccgca gcgcggtctt gatcacgtgg
      961 cgctccgcct cgacgacccg gggatggctc cactgcccgg gcaggccgat gctcatcgag
     1021 tagtcggcgg ggccgaactg caccatgtcc acccccttca ccgacagcat cgcctccagg
     1081 ttctccaccg cggaggcctt ctcgatcatc accgccacca ccgcgtcgtc gagcgcctgg
     1141 acgaacgcgg gcgagcccac gtccatcacg taaccgacgt cgcggcgcat gcccactccg
     1201 tggatgccgc ccgcctcggg cgtctcggcg cgcacgctcc tcacgcactc gcgcacgtcc
     1261 tcgggcgtgc gcggatcggc gaagagcacg ttctggatgc ccgagccgat ggcccgcacc
     1321 gccaggtacg tgcggggctg ctgctcgacc ttgaacatgg ccgacatgtg gtcgaaggtg
     1381 ttcaccgccc gcccgaggtt ctcgagcgcg aagagatcgt agggcgcgta ctcgcccgcg
     1441 aactccacgt agtcgaagac ccccgcgtgc ccggccagct ccaccaccgc cggccacgcc
     1501 gagtggatgt gcgtgcccag cgtgggccgt cccgcccgca gcagctcccg gagtcgattg
     1561 cgtcgcatgc gccgagtcta gggggggcag gtgcgccccg tcaatcaccg cgctatactc
     1621 gcacggtgcg cgacgccccc ggcccgctct ccgacctcgt agtgctcgat ctcacccggg
     1681 tgctcgcggg tccctactgc acgcgcctgc tcgccgatct gggcgcgcgc gtgatcaaga
     1741 tcgagcggcc cggcgagggc gacgacatgc ggaagaacca cctccagctc gagcccggcc
     1801 gcgccgacca gtccacctac ttcatccgca tcaacgtggg caaggagagc gtcgcggtgg
     1861 atctcggcca cccgcagggc cgcggggtcg tggccgatct ctgccgcgtc gccgacgtct
     1921 tcgtcgagaa cttcgcgccc ggcgtcgtcg agaagctcgg cctgggctac cccgcgatgg
     1981 ccgcgatcaa gcccgacctc gtctactgct cgatctcggg cttcggccag accgggccgt
     2041 ggcgcgagcg ccaggccttc gcccacatca tcaacgcggc ctccgggatg atgcatctcg
     2101 agcagggcga tcagccggcg ccccgcgcgt cgaatctcca ggccgccgac gtgctggcgg
     2161 gcacccacgc cttcggcgcc atcctggccg ccctgcggcg acgcgatcgc accggtcagg
     2221 gcgcctacct cgacgtctcg atgctggaag cgttgatcgc ggccgacgac gtctcctacg
     2281 cggcggtgct gaacggcggc gaggcgcagg gctccccgcg cccggcgatg ggcgtcttcg
     2341 cggtgggcgg ccggcatctc gcgctccaga ccggcggcgc cgccgcgctg tggccccgtc
     2401 tgcaggcgct gctctcccgg cccgatctgg ccaccgatcc gcgcttcgcg acaccggatc
     2461 tgcggcggcg caactggtcc gcgctgcgcg acatcatcgg cgagtggctc gcgggcttcg
     2521 ccacggtgga cgaggcgctg gaggcgctcc gcggcgcccg cattcccgcg gccgccgtgc
     2581 tcatgcccga ggaggtcgtc gcgcatcccc acctgcgggc gcgcggggcc ttccccaccg
     2641 tgccgcatcc cgcccgcggc gacgtgcgcg tcaccgcctc gccgttccag ctcgacggcc
     2701 ggccggtcac gcccgcgggg ccggcgccgt accgggtcgg cgagcacacc cggtccgtgt
     2761 tgacagagct gctcggttac aaggaacgcg agattgacga tctcgctcag gccggcgccg
     2821 tgcaggcgcc ctgagacggg accacaggag agacgagaca tggcggcgac accgatgacc
     2881 ggcggcgaag cgctggtgcg ctccctctac accgaaggcg tccgcgtgat cttcgggctg
     2941 cccggcgtgc agctctacgg cgtgatggcg gccctgcgcg acgagccacg catccgcttc
     3001 atcaccacgc gccacgagca ggccacgagc ttcatggccg acggctacgc gcgcgcgggc
     3061 ggcggcttcg gcaccgccct cgtggtgccg gggccgggcc tgctcaacgc gatgtcgggc
     3121 ctctccaccg cctactcggc ctcctcaccg gtgctgatcc tcgccggcca ggtcccccgt
     3181 gacagcatcg ggaaggacat cggcctgctc cacgaggtca acgaccagct cgactgcatc
     3241 acgccggtca ccaagtggcg caagcgcgtc ctgcaggtcg ccgacattcc cgccgcggtg
     3301 cgggaggcgg tcatccagct ccgatcaggc cggccacgcc cggtcgagct cgagatgccg
     3361 cccgagacga tggaggacga ggggctcgcc gagctgctgc cgcccaccga ggtcccccgg
     3421 cccgccgcgg ccgcgcgcga cgtggaccgc gccgccgagc tgctgctcgg cgcgcgcgcg
     3481 ccgctgatct acgcgggcgg cggcgtccac ctctccggcg cccacgacgc gctggccgcg
     3541 gtggccgagc acctgcaggc cggcgtggcc cagtccgccg agggcaaggg cgcggtgagc
     3601 gaccacaatg cgctctcgct cggcgccgcc tactggcgcg actcgccgct gcgcaatcac
     3661 gtgcaggaag cggacgtggt gctggcggtg ggcacgcgcc tggctctggt gaccttcaag
     3721 ccgggaacgc gcatcgtcca gatcgacgcg gacgagcgcg agatcgggcg caaccacaag
     3781 gacacgctcg gcctggccgg cgacgcgcgg gccaccctgg aggccctgct cgagcggctg
     3841 cgcgcggcgt ccccgccccg gccctcgcgc aaggcggagc acgaggcgct ccgcgcgagg
     3901 atcgccgccg acaacacgat ggagcccaac gcctccatca tcaaatcgct ccgcgcaagc
     3961 acccccgaga acgccatcct ggtcgccggc atgacccaga tcggctacta ctcgcgcccg
     4021 ttctggccgg tctacgagcc gcggacgtat ctctcctcct cctactccgg caacctgggc
     4081 tacgcctatc cggtggcgct cggggcgaag gtcgcccgcc cggaccgccc ggtggtctcg
     4141 gtctccggtg acggcgggtt cctcttcaac gcgcaggagc tctccaccgc cgcccgctac
     4201 ggcatcaacg tggtcgcggt cgtcttcaac gacagctcgt acggcaacgt ggcgcgggat
     4261 ctcgacgagt cctggggcgg ccagtacggc gccgatctca agaacccgga cttcatgaag
     4321 ctggccgacg cctacggcgt gctcggcctg cgggccaagg agccgaccga ggtcggccgc
     4381 ctcgtgcgcg aggccatcga ccgggatcgc ccggcgctcg tcgaagtgcc ggtcggccgg
     4441 atgaatcgcc cgatgttctt cgccccgcgg cggacgccga ccaagcaccc gcgctagcga
     4501 gacagccggc ggaggatcca gagcatggca gcggccgcgg tcgagcgcgg ctccgcggag
     4561 cggttgcggt tcctggacgc ggtgcggggc ttcgccctga tcctgatgat cctgaatcac
     4621 accgggcgct ggtggcagga cggcgccatg ggctggccgc gctacaacct gatctacgcg
     4681 tccatggcgg tgggcgcgcc gatcttcctg tttctcgtcg ggttctgcct gccgctgtcg
     4741 ttcgcgccgc gccggcaggc gggcgccccg gtcggcgccg gggtggtcgc gaagttcctc
     4801 cagcgcgggg cgaagctcat cctggcgggc tgggcgctca atctcttcgt gttccgcgac
     4861 gagccgttct gggagggcgg cgtgctccag accatcgggc tcggcatcat cctggccctg
     4921 cccccgatgc tggtgttgca ccggcgggcc gcccgctacg ggctcgcggc cttcgcgctc
     4981 gccttctacg ccctgttctc cgtcctgcac caggaggtga cggcctggct gcccgcccat
     5041 cgcgtgctgg ggcgcatctt cttcttcgag ttcgcgccgt ggccgtggat cgccatcgtg
     5101 tggatcgggc tggccctggg ttgggtcttc gcgctccagg ataccgccga gcgacgcgcg
     5161 cgttacctga cgtggatgac ggccgccggg ctcgtctgcc tcgcggtctt cgcgatctgg
     5221 gactgggcgc acggcacccc gatccatctc tcgctcgcct tcaagcgcga cttcatcgtc
     5281 aacaaccact ggatcgcgcg cgggatgacg agcttcctgt gcgtgggcgc ggtcttctgc
     5341 ctgctgggcc tcgcctacta cgcggtcgag gtcaagcgcc tgcccgccgg atggctggtg
     5401 atcctggggc gcaccgcgct gatgctctac ttcctccacc acctgatcgt gctcacgctc
     5461 gccaaggaat tgctcggcct caagctgaat aactggtggt ggtacgcact cgcgaacgcg
     5521 gtgctgctgg ttctcctcgt cggaatcggc cggctgtggc tgggcatccg cggcttcacg
     5581 gcccggcggc gcctccagcc tgcgtagcct cggtgctctt ttccggccgg ggcctgacga
     5641 ttttcggagc ctcgttccgc cttcgacgct gaagcggctg atttccctgg cgcgttccgc
     5701 tggcccgcgt cttgctctat cagcgggcat gagcgctccc gcggcacgtc cgaagattct
     5761 cgtggtggac gacgagccca tcatcgcgca gctcatcacc gacgtgctga ccggtgaggg
     5821 gtacgacgtg gataccgcgt cggacggcct cgtggcgctc gagtacatcg gccgccacgg
     5881 gtacgatctc atcctcagcg acctgcgcat gcccggcctc gacgggctcg gcctctaccg
     5941 tgagctggac cagagccggc ctgagctggc acgccgcttc gtcttcatca ccggcacctc
     6001 cgagcacacc gactacgcgg gcctcatcgg ggacctcgcc gtgcccgtcc tcaccaagcc
     6061 gttcgacatg tcggcgctcc agcgcgtcgc ccgggagact ctggccgctt cggtttcctg
     6121 accccgtccg gctcctgacg gacgtcccgc cgctgacaag ccgcgtcacc ccgcgacccc
     6181 gcggggctca caaattcggc cccttagccg gcatcatgcc tggcatacgc catgctgtgg
     6241 ttccagtcgg ggagcgcagt gaccgtgcgt ccggtctacc gagcgcaggt cccccggagg
     6301 gaaccatgca aaaggcactg gccatgtggc tcctgctcgc ggcgagcggc ggagcgcaac
     6361 ccgaagtgag cccgacccag gtggtccaga cggccaccga gcaggtcttg cagcttgtcc
     6421 aggatgggca gctggcggct ccggtctccc aggaccagcg gcgcatcgaa gtccagcgga
     6481 tcgccgatcg gctgttcgac tttcaggaga tgtcccggcg ctcgctggcg ctgcactggc
     6541 gcgagcgcac cccacaggaa caggccgagt tcgtcgcggt gttcaagcag ctcctcggcc
     6601 gcgcctacat cggacggctg gagaactact cgggcgagca gatcgtgtac ctgagcgagc
     6661 acgtggacgg cgagttcgcc accgtccgct ccaagatcgt caccgggcgc ggcgcggagg
     6721 tcccggtgga ctaccgtctc caccaggtcg gcgcccgatg ggtcgtctac gacgtcgcgg
     6781 tgtccggcgt gagcttcgtc gcgaactacc gggggcagtt cgacaagatc atccggggct
     6841 cgtcgttcgc ggccctcatg cgcgacctga agggaaagtt cgcggagacg accggccgct
     6901 ccgcggtccc tgccgtggcg cccgccgtgg ctcccgtcat cgcgccagcc gtcgcacccg
     6961 caccgaagaa cccgtagccc gcccgaggcc ggcgggccgg gcgtcgggcc cgctcgaggt
     7021 cctcggcgcg gaccggcagc gcccgatcag cacgaagatc gcgttgccga gcgccggcgc
     7081 caccggcggc acgccccgct ctcccagccc tcccggtcgc ttccccgtgc acgctcgaac
     7141 ggccggcgtg tagccccgaa ttcgcggggg cactctgata gactcggccc agccgaaagc
     7201 ggccgcctcg ttcccggggc cggcccgacc tcaaggagga gtccgatgac ccgagcgtgg
     7261 gcggggctgc tggtctgggt gctcgcggcc ggtcttctcg tggggtgcgg ccgcagcaag
     7321 gaagacctcg tccagaaatc gcggggcgtc ggcacgcgcg cggagctcga gcaggcgctc
     7381 ggcaagccga gcgacatcag caagctgggc ccggtggagc agtggaccta caaagcctcg
     7441 aacgggaacg tggtcttcgt catcgtgggc gacaaggtca cgatgcaggc caccacgacc
     7501 ccggacgaga agaaagacca gaagtaggag gactcgacgt gagcgaatcc ctatccgacc
     7561 atctcacgca gcggctcgat cgcctggagc gcgagaaccg gcgcctcaag ctccttggcg
     7621 gcctcgcgct gctcgggctc gccgctctga ccgtgatggg ccagacggcg cccacctcgg
     7681 tggccaacac cctcgaggcg gagcgcttcg tgctccgcga caacgcgggc aacgtgcgcg
     7741 ccaccctcgg cctccgcccg gacggcaccg ccgcgctggc attggccgac gatacccagc
     7801 aggaccgcgc ggtgctgagc gtcaccgcgc tcggccaggc gggtgtcgat ctctccgatc
     7861 gcgcgggacg cctgcgcgcc ggcctgggcg tgcgcccgga cggctccgcg ctgctcaccc
     7921 tgctcgacca gaccgatcgg ccgcgcgtcg agctccggac atacggcgac ggccgcccga
     7981 gcctcgccct gctcgaccag ggcggccggc cccggtcgct gctcgcgctc accgccgagg
     8041 gcgccaccgg gctcaccctc tacgaccgca acgggcgccg acgcagctcg atgggcacgg
     8101 agccggcggg cgcgcccgcg gtgtggctct acgacaccga gggcaacggc cgcgcgatgc
     8161 tcgcagtcac cggcgacggg cagcccacgc tcaatctcac cgacgccggc ggcaagggac
     8221 ggctctggct gcgcgtgagc gcggatttcc agctgccgtt cctgagcctg cacgatcgtg
     8281 agggcaggca gcgcgtggcc atgctcctgc aacagggtgg cgtgccgagg ctggccctcg
     8341 gcgacgcctc cgagcgggtg atctggaagg ccccctaggc ccgcatgccc ggcaccaacg
     8401 ccctggtgct ggccggcctc gtcgtctacc tggccgccag cctcctgctc tggcggctgc
     8461 tcctcaagga atcgccgccg atgggacggc gccggagccg gccgcgctgg ccgcgccgtc
     8521 tcgcgaaggc cttcgccacc ctgctcggcg ggatcgccgc gggtgcggcc tcgctgctcg
     8581 tcgtcgcgac cgtcgtcaac gcgggcaagc ccggcaccct cacccagaac gagatgcgcg
     8641 ccgtgtgggc ggtggcgtcg gtgatcgcga tgctcttcat cgggcgcgcc ccgctgatcc
     8701 gccgggtggt gacgcgcgcc tgcctcgccg tgggggtgca gggcgtggtc ctgccgctgg
     8761 ccaccctcct gtccttcgtc gtggcgggaa cgcgcctggc cggctccgca ggcaccaacg
     8821 gcgagcggac cgtggaagtg ttcggagtgc ggctggtcgg caacctgccc gcggtctgga
     8881 tcggactcgg cgggttctgc atcggcctgg ccctcgtgat cgtgggcgac cgggcccacc
     8941 ggcaggtcgc caagcgccga gcccgtcgcc gcttccgctc ctcgcgggcg ggcgtgatct
     9001 ccgcgcgccg tcgccaccgc taggagggcg ctcccaaagg cccgtctgct tcgttgggcc
     9061 cctcggtcgg ggcgctcaac gcgcgcgctc ggttcgctgc cggcggtcgc gggcttcgtt
     9121 ccgcacgagg gcgacgtgca ggcggcagtg gtagacatcg cgccggtgct ggagcgagag
     9181 acggcgcgcc agcccctcga tctggccgcg cagcgactcg agccgcgcga cttcctgccg
     9241 cagcgtctcc ggccccggca gaaccgccgc gaccgcccag agcgcgagcc ggcccttcac
     9301 cgtcgcctga actcctcctt catcgcggcg agc
//
LOCUS       DASUFA010000077         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1154371, whole genome shotgun sequence.
ACCESSION   DASUFA010000077 DASUFA010000000
VERSION     DASUFA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154371"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VFX87_03070"
     CDS             <1..1348
                     /locus_tag="VFX87_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4993917.1"
                     /translation="IRRVERLNPRLNAVVTALFDEARRAAGLPPGHGAFAGVPFLLKD
                     LLAEYAGTRLTEGSAFLDGHYVSPHDTELVARYRRAGLIVIGKTNTPEFGILPTTEPR
                     LFGPCRNPWDPRRTTGGSSGGSAAAVAAGMVPMAHANDGGGSIRIPAACCGLFGLKPT
                     RGRNPLGPHYGDMFSGLVAEHAVTRSVRDSAALLDATAGPAPGDPYVAPPPARPWLEE
                     VGRDPGRLRIAVNRAAPNGAAVHPDCVAAVDEAARLAAALGHHVAEAAPVLDGQMLSE
                     AFFTLWSAGCAWTVADWARRTGQTPDPARFEPLTWVLYEMGQRRSAAEYLAAVQDLQR
                     VSRDVARFFGDWDVWLTPTLAEPPLPLGSFDSTPDDPIRGLRCAAAFVPFTPLVNVTG
                     QPAMSTPLAWNAEGLPIGTHWVARFGDEATLFRLAAQLEAARPWVARRPPLAETPA"
     gene            1422..1781
                     /locus_tag="VFX87_03075"
     CDS             1422..1781
                     /locus_tag="VFX87_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4993918.1"
                     /translation="MTACRLAVILFATALLLAEGAWSAHPAAPDTQERIALDNDTVKV
                     GHHVYPPGASSGIHVNPEPEIGIVVEGELTVVTRHGKQIYKAGSVIYLESGSGHIALN
                     ETKSPVKFWALNLKKCD"
     gene            complement(1801..2637)
                     /locus_tag="VFX87_03080"
     CDS             complement(1801..2637)
                     /locus_tag="VFX87_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4993919.1"
                     /translation="MGGEGSRFQRALSYAVLTALGLIVLFPFYWMTVTSFKGEDQMRS
                     LVSMFWPSPFLLENYQLLLAKTEFAAWYGNSVFVAVSSTLLATAVGTIGAYALARLKF
                     LGRGFMASATLITYLVPPSILFIPLYAQIKTLGLANSLSGLILSYPSFTVPFVTWLLM
                     GYFESIPEELEEAAMIDGATRFGAFRRIILPLAAPGVLAAALYAFTQAWNEFLYSLVF
                     ITDARLRTLPVGLASFITGDVYGWGYLMAGAVLTTLPVIAAYMYLQRYMVEGLTAGSV
                     KG"
     gene            complement(2637..>3031)
                     /locus_tag="VFX87_03085"
     CDS             complement(2637..>3031)
                     /locus_tag="VFX87_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX4993920.1"
                     /translation="VNVWRGFPFFGISFLAGMKAIPGELYEAAAVDGATPLQRFRHVT
                     LPGIKNITIIVVLLSTIWTFNDFGIVYILTKGGPGGTTMVMPVLTYETAFGAQRLGEA
                     IAVALYLLPGLALTIVVLARYMKKGRTR"
BASE COUNT          479 a         1092 c         1032 g          428 t
ORIGIN      
        1 gatccgccgc gtcgagcgcc tcaacccgcg gctgaacgcg gtggtcacgg cgctgttcga
       61 cgaggcgcgc cgggccgccg gcctaccgcc cgggcacggg gccttcgcgg gggttccctt
      121 tctcctcaag gatctcctcg ccgagtacgc gggcacccgg ctcaccgagg gctccgcctt
      181 cctcgacggc cactatgtct ccccgcacga caccgagctc gtcgcgcgat accgccgcgc
      241 cgggctcatc gtgatcggca agaccaatac gccggagttc ggcatcctgc ccaccaccga
      301 gccccgtctc ttcggtccct gccggaatcc ctgggatccc cgccgcacca ccggcgggtc
      361 gagcggcggc tcggccgccg cggtggcggc cggcatggtc ccgatggccc acgccaatga
      421 cggaggcggc tccatccgta ttccggcggc ctgctgcggc ctcttcgggc tcaagcccac
      481 tcggggccgc aatccgctgg gcccgcacta cggcgacatg ttcagcggtc tcgtggccga
      541 gcatgcggtg acccgatcgg tgcgggacag cgcggccctg ctcgatgcca ccgcgggacc
      601 ggcgccggga gatccctacg tggcgccgcc gcccgcgcga ccctggctcg aggaggtcgg
      661 gcgcgatccc ggccggctcc gcatcgccgt caaccgggcg gcgcccaacg gcgcggccgt
      721 gcatcccgac tgcgtggccg cggtggacga ggccgcccgg ctcgccgccg cgctcggcca
      781 ccacgtggcg gaggcggcgc ccgtcctcga cggccagatg ctcagcgagg ccttcttcac
      841 gctgtggtcg gcgggttgcg cctggacggt ggcggactgg gcgcgccgca ccgggcagac
      901 gcccgacccc gcgcgcttcg agccgctcac ctgggtgctc tacgagatgg gccagcggcg
      961 ctccgccgcc gagtatctcg ccgcggtcca ggacctccag cgagtctccc gcgacgtggc
     1021 ccgcttcttc ggcgactggg acgtctggct caccccgacc ctggccgagc cgcccttgcc
     1081 gctgggcagc ttcgactcca cccccgacga ccccatccgt ggcctgcgct gcgcggccgc
     1141 cttcgtgccg ttcaccccgc tggtcaatgt gacgggacag ccggccatgt ccactcccct
     1201 ggcctggaac gccgagggcc tgcccatcgg cacccactgg gtcgcccgct tcggcgacga
     1261 ggccacgctc ttccggctcg ccgcgcagct cgaggccgcc cgaccctggg tggcgcgccg
     1321 ccccccgctg gcagagactc ccgcatgagg cgccggcggc tcgccgcgct cgctatcgtg
     1381 gcagctctcc ggctgattca tcccgagagg agactccccg tatgaccgcc tgccgcctcg
     1441 ccgtgatcct gttcgccacc gccctgctcc tcgccgaggg cgcctggagc gcgcatcccg
     1501 ccgcgccgga tacccaggag cgcatcgcgc tcgacaacga caccgtgaag gtcggccacc
     1561 acgtctatcc gccgggggcg tcatcgggga tccacgtgaa cccggagccc gagatcggga
     1621 tcgtggtgga gggggagctg accgtcgtca cccggcacgg caagcagatc tacaaggcgg
     1681 gctcggtgat ctatctcgag tcggggtcgg ggcacatcgc gttgaacgag accaagagcc
     1741 cggtgaaatt ctgggcgctc aacctgaaga agtgcgactg agcgcgacgg cgcggccgcg
     1801 ctagcccttg acgctccccg cggtgagccc ttccaccatg taccgctgga ggtacatgta
     1861 ggcggcaatg accggcaggg tcgttagcac cgcccccgcc atcaggtagc cccagccgta
     1921 cacgtccccc gtgatgaagc tcgccagccc caccgggagc gtgcggaggc gcgcgtcggt
     1981 gatgaagacg agcgagtaga ggaactcgtt ccacgcctgg gtgaaggcat agagcgcggc
     2041 ggcgaggacg ccgggggcag ccaggggcag gatgatccgc cggaaggcgc cgaagcgcgt
     2101 ggcgccgtcg atcatcgccg cctcttccag ctcctccgga atggactcga aatagcccat
     2161 gaggagccag gtgacgaagg gaaccgtgaa cgacggatag ctgagaatca gaccggagag
     2221 gctgttcgcg aggcccaaag tcttgatctg ggcgtagagc gggatgaaaa ggatggacgg
     2281 cggaacgagg taggtgatga gcgtcgcgct cgccatgaag ccccggccga gaaacttgag
     2341 tcgcgccagc gcgtaggcgc cgatggtccc cacggcggtc gccagcagcg tgctggagac
     2401 ggcgacaaac acgctgttgc cgtaccacgc cgcgaactcg gtcttggcca ggagcagctg
     2461 gtagttctcg agaaggaagg ggctgggcca gaacatggag accaggctcc gcatctggtc
     2521 ctcgcccttg aacgacgtga cggtcatcca gtagaacggg aagagcacga tgaggccgag
     2581 cgcggtcagc accgcgtacg acagcgcgcg ctggaagcga ctcccttcac cgcccactag
     2641 cgcgtcctcc ccttcttcat gtagcgggcc aggaccacga tggtcagcgc gaggccggga
     2701 agcaggtaga gcgccaccgc gatcgcctcc cccagccgct gggctccgaa cgcggtctcg
     2761 taggtcagga ccggcatgac catggtcgtc ccgccgggac cgcccttggt caagatgtag
     2821 acgatgccga aatcgttgaa cgtccagatc gtcgagagca gcaccacgat gatggtgatg
     2881 ttcttgatcc cgggcagggt gacgtggcgg aaccgctgca gcggggtcgc gccatccacc
     2941 gccgccgcct cgtagagctc gcccgggatg gccttcatcc ccgccaggaa ggaaatgccg
     3001 aagaagggga agccgcgcca cacgttcacc g
//
LOCUS       DASUFA010000078         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1506886, whole genome shotgun sequence.
ACCESSION   DASUFA010000078 DASUFA010000000
VERSION     DASUFA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506886"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="rsmB"
                     /locus_tag="VFX87_03090"
     CDS             <1..1230
                     /gene="rsmB"
                     /locus_tag="VFX87_03090"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX4993921.1"
                     /translation="LDARDAGLCTEIVYGTLRWRRHLDWRLTPHLNRPLAKLDPWVRA
                     VLRLTAYQLLFLDRVPRWAAVDEAVSIARLKSRNPGPAEFVNAVLRAFTRAPAPPRLP
                     ADPVEAAAIRWSFPDWMAARWVARYGLPEAERLMAGLNERPPVTIRANTLRISREDLA
                     TRLRDEELADTEPTSLAPEGLTVRRGGVGRWAAFASGWCTIQDEASMLIAHPLDPQPG
                     ELVADTCAAPGTKTTHLAQLMANRGRIIAMDPHAGRLGLLTQAVSRLGVSIVEAHAGG
                     VASVSGRWKGRCDRVLVDAPCSNLGVLRRNPDVKWKREEAELGRLAEKQRGILTASAG
                     LAKPGGRLVYATCSLEPEENDAVVRAFLDEHPDWRVDPPATFPVAPDADGVIRCLPHV
                     HGTDGFTAVRLARRPRA"
     gene            1276..1938
                     /gene="rpe"
                     /locus_tag="VFX87_03095"
     CDS             1276..1938
                     /gene="rpe"
                     /locus_tag="VFX87_03095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX4993922.1"
                     /translation="MMKIAPSILSADFAALGEAIARVETAGADLLHVDVMDGHFVPNL
                     TIGPPVIESIRKRTRLPLDVHLMIEEPERWVETYVKAGADYVTVHAEACAHLERALTM
                     IREAGARSGVALNPSTPPEVLEYVLGDLDLVLVMSVNPGFGGQSFIPTTYEKIRRLRA
                     MLADRPVLISVDGGVKADNAGPLAQAGATVLVAGSAIFGAPDPGAALAGLRSASEVLR
                     RA"
     gene            2004..3347
                     /gene="ffh"
                     /locus_tag="VFX87_03100"
     CDS             2004..3347
                     /gene="ffh"
                     /locus_tag="VFX87_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX4993923.1"
                     /translation="MLDSLTNRLTGILDRLRGFGRLTEENIQEALREVRVALLEADVN
                     FKVVKGFIDRVRVKAVGQDVLQSLTPGQQVVKVVRDELVELLGGSAHRLAMAPHPPTV
                     VMLVGLQGSGKTTSAAKLARHFQKQGQHPLLAAADVYRPAAVDQLKTLGAQLGIPVVG
                     EATQRPVEICAAARDEAARRGLSPLILDTAGRLHIDEEMLEELRAIKRAVGPHHVLLV
                     VDAMTGQDAVTVAEKFNAAIGIDAVILTKMDGDARGGAALSVRQVTGRPIAFVGVGEK
                     TEALEPFHPDRLAQRILGMGDVLSLVEKAQATVDAGQAEALAQKIRDDTFTLEDFAAQ
                     LRQLRSMGPLGQLMDMVPFFKGAKGLPKEMTGEEQELDRYGAIIASMTPHERREPSVI
                     NGSRRARIARGSGTSVSDVNRLLKQYAQLRKMMKGLKNMEGRMGKFKGALPFLPR"
     gene            3393..3653
                     /gene="rpsP"
                     /locus_tag="VFX87_03105"
     CDS             3393..3653
                     /gene="rpsP"
                     /locus_tag="VFX87_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX4993924.1"
                     /translation="MAVHIRLRRTGTTRKPAYRVVVADSRAARDGKFIEVIGHYNPLT
                     EPPTIKIEVEKAQAWIAKGAQPSNTVKNLLARARAAAGAVKA"
     gene            3661..4182
                     /gene="rimM"
                     /locus_tag="VFX87_03110"
     CDS             3661..4182
                     /gene="rimM"
                     /locus_tag="VFX87_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4993925.1"
                     /translation="MTGAPLVTIGEVLRPFGLRGEVKVRPLTDRPRERFKALMSCTLW
                     EPQGDRREPCRIASCRFEGETVYLRMDGVDSPEAARRFCGRLLAVSREEALPLQEGSF
                     YPWQLEGAVVETRDGREVGRFVGVEPGAQELWLVDDGTQRRLIPAVPEIVVEVSVADR
                     RIVIDPPEGLLDL"
     gene            4179..>4365
                     /locus_tag="VFX87_03115"
     CDS             4179..>4365
                     /locus_tag="VFX87_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX4993926.1"
                     /translation="MRIDIVTLFPGMLEAPLAESILGRARARGLVDIRVHDLREHATG
                     RHRVTDEPPFGGGGGMIL"
BASE COUNT          633 a         1514 c         1611 g          607 t
ORIGIN      
        1 ctcgacgccc gcgacgcggg tctctgcacc gagatcgtct acggcacgct gcggtggcgc
       61 cgtcacctgg actggcgcct caccccgcat ctcaaccgcc cgctcgccaa gctcgacccc
      121 tgggtgcgcg cggtgctgcg cctcaccgcg taccagctcc tcttcctcga tcgcgtgccg
      181 cgctgggccg cggtggacga agcagtgagc atcgcgcggc tcaagtcccg caatccgggg
      241 cccgcggagt tcgtcaacgc ggtgctgcgc gccttcacgc gggcgccggc cccgccccgc
      301 ctgcccgccg acccggtcga ggcggccgcc atccgctggt cgtttccgga ctggatggcc
      361 gcgcgctggg tcgcccgcta cgggctcccg gaggcagagc ggctcatggc ggggctgaac
      421 gagcgcccgc ccgtgaccat ccgcgcgaac accctccgca tctcccggga ggacctcgcg
      481 acccgtctcc gcgacgagga gctggccgac accgagccca cgtcactcgc gccggagggc
      541 ctcacggtgc gccggggtgg ggtcggccgg tgggccgcct tcgcgtccgg ctggtgcacg
      601 atccaggacg aggcgtccat gctgatcgcg cacccgctgg atccgcagcc gggcgagctg
      661 gtcgcggaca cctgcgccgc gcccgggacc aagaccaccc acctggccca gctcatggcc
      721 aatcgcggac gcatcatcgc catggatccg cacgcggggc ggctcgggct cctgacccag
      781 gcggtctccc ggctcggcgt ttccatcgtc gaggcccacg cgggcggcgt ggcctcggtg
      841 tccgggcgct ggaaaggccg ctgcgaccgg gtgctcgtgg acgcaccctg ctcgaatctg
      901 ggcgtgctgc gccgcaatcc cgacgtgaag tggaagcggg aggaggccga gctcggccgg
      961 ctcgccgaga agcagcgggg cattctcacc gcctcggcgg ggctcgcgaa gccgggcggg
     1021 cgcctggtct atgccacctg ctcgctcgag cccgaggaga acgacgcggt cgtgcgggcc
     1081 ttcctcgacg agcatcccga ctggcgggtg gatccgcccg ccaccttccc ggtggcaccc
     1141 gacgcggacg gcgtgatccg ctgcctgccc cacgtccacg gcaccgacgg cttcaccgcg
     1201 gtgcgcctcg cgcggcggcc gcgggcctga ccccggccct ttgcggccct cggcgctttg
     1261 gagtaaggtc gtcgcatcat gaagatcgcg cccagcatcc tgtccgccga cttcgccgcg
     1321 ctgggcgagg ccatcgcgcg ggtagagacg gccggggcgg acctgctcca cgtggacgtc
     1381 atggacggcc atttcgtgcc caatctcacc atcgggccgc cggtgatcga gtccatccgg
     1441 aagcggacgc ggctccccct cgacgtgcac ctcatgatcg aggagccgga gcgctgggtg
     1501 gagacctacg tgaaggcggg cgccgactac gtgaccgtgc acgccgaggc gtgcgcgcac
     1561 ctcgagcgcg cgctcacgat gatccgcgag gccggggccc ggtcgggcgt ggcgctgaac
     1621 ccgtccaccc cgccggaagt cctcgagtac gtgctgggcg acctggacct ggtgctggtc
     1681 atgtcggtca acccgggctt cggcggccag tcgttcatcc ccaccaccta cgagaagatc
     1741 cgccgcctcc gggccatgct cgccgaccgg ccggtgctga tctcggtgga cggcggcgtc
     1801 aaggccgaca atgccggccc gctcgcgcag gccggggcca ccgtgctggt ggcgggctcc
     1861 gccatcttcg gcgcgcccga tccgggcgcc gcgctggccg gtctgcggag tgcgtcggaa
     1921 gtactgagaa gggcttgact tttccccgac tcgtcagtat cctttagcgt ttctacaaac
     1981 ctgtcccctg gtggtagaac acgatgctcg atagcctgac caatcgtctg accggcatcc
     2041 tcgatcggct ccgcggattc ggccggttga cggaggagaa catccaggag gctctgcgcg
     2101 aagtgcgcgt ggccctgctg gaggccgacg tcaacttcaa ggtcgtgaag ggcttcatcg
     2161 atcgggtgcg cgtgaaggcg gtcgggcagg acgtgctcca gtcgctgacg cccggccagc
     2221 aggtggtcaa ggtggtgcgc gacgagctgg tggagctgct cggcggctcc gcccaccggt
     2281 tggcgatggc gccccatccc ccgaccgtgg tgatgctggt gggcctgcag ggctccggca
     2341 agacgaccag cgccgcgaag ctcgcgcgcc acttccagaa gcaggggcag catccgctcc
     2401 tcgcggccgc cgacgtgtac cggcccgcgg cggtggacca gctcaagacc ctcggcgccc
     2461 agctcggcat cccggtggtg ggcgaggcca cgcagcggcc ggtggagatc tgcgcggcgg
     2521 cccgggacga ggcggcgcgc cggggcctgt ccccgttgat cctcgacacg gcggggcggc
     2581 tgcacatcga cgaggagatg ctcgaggagc tgcgggccat caagcgggcg gtcggtccgc
     2641 atcacgtcct gctggtggtg gacgcgatga ccggccagga cgcggtgacg gtggccgaga
     2701 agttcaacgc ggcgatcggc atcgacgcgg tgatcctcac caagatggac ggagacgccc
     2761 ggggcggcgc cgcgctctcg gtccgccagg tcacgggtcg gcccatcgcc ttcgtcggcg
     2821 tgggcgagaa gacggaagcg ctcgagccat ttcacccgga ccggctcgcc cagcgcatcc
     2881 tcggcatggg cgacgtcctg tcgctggtgg agaaggccca ggccaccgtg gacgccgggc
     2941 aggcggaagc cctggcccag aagatccgcg acgacacgtt cacgctggag gacttcgcgg
     3001 cgcagctcag gcagctccgc tccatgggcc cgctcgggca gctcatggac atggtgccgt
     3061 tcttcaaggg cgccaagggg ctgcccaagg agatgacggg cgaggagcag gagctggacc
     3121 ggtacggagc gatcatcgcg tcgatgaccc cgcacgagcg gcgcgagccg tcggtcatca
     3181 acggcagccg gcgcgcccgc atcgcgcggg gaagcggcac gagcgtgtcc gacgtgaacc
     3241 ggctgctcaa gcagtatgcc cagctccgga agatgatgaa ggggctcaag aacatggagg
     3301 ggcggatggg caagttcaag ggcgccctgc ccttcctgcc gcgctgaccc cggaccgacg
     3361 cagaccacga caaccgaaca gggaggtgac cagtggcggt gcacatcagg ctgagacgga
     3421 cggggacgac gcggaagccg gcataccgcg tggtggtggc ggactcgcgc gcggcgcggg
     3481 acggcaagtt catcgaggtg atcgggcact acaacccgct cacggagccg ccgacgatca
     3541 agatcgaggt cgagaaggcg caggcgtgga tcgcgaaggg ggcccagccc tccaacaccg
     3601 tgaagaatct gctggcgcgg gcgagggccg ccgcgggagc cgtcaaggcg tagggatccg
     3661 gtgacgggag cgccgctcgt gacgatcggg gaggtgctgc ggcccttcgg gctccgcggc
     3721 gaggtcaagg tgcgtcccct caccgaccgg ccgcgcgagc ggttcaaggc gctcatgtcg
     3781 tgcacgctgt gggagcccca gggggaccgg cgcgagccgt gccgcatcgc ctcctgccgc
     3841 ttcgagggag aaaccgtcta tcttcggatg gacggcgtgg actcgccgga ggcggcccgc
     3901 cggttctgcg gccggctgct ggcggtgagc cgggaggaag ccctgccgct ccaggaaggc
     3961 agcttctacc cgtggcagct cgagggcgcg gtggtcgaga cgcgcgacgg gcgggaggtg
     4021 ggccggttcg tgggcgtgga gcccggcgcg caggagctgt ggctggtgga cgacggcacc
     4081 cagcggaggc tcatcccggc ggtgcccgag atcgtcgtgg aggtcagcgt ggcggatcgc
     4141 cggatcgtga tcgatccgcc ggaagggctg ctcgacctgt gaggatcgac atcgtcaccc
     4201 tgttcccggg aatgctggag gcgccgctcg ccgagtccat cctggggcgg gcccgcgcgc
     4261 gcggcctcgt ggatatccgt gtgcacgacc tccgggagca cgcgaccggg cggcaccggg
     4321 tgaccgacga gccgcccttc ggcggcggcg gcggcatgat cctca
//
LOCUS       DASUFA010000079         5099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_820199, whole genome shotgun sequence.
ACCESSION   DASUFA010000079 DASUFA010000000
VERSION     DASUFA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820199"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFX87_03120"
     CDS             complement(<1..386)
                     /locus_tag="VFX87_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993927.1"
                     /translation="MAAAIEVQQFAEARARRAPVTAAGAVFGDETRTLQGLLDEGGAE
                     ADAVLPARELVEVPHIEPLVAVAVEGEQALDLGHRGPLGRGPLPAAIEQPIIAVALQL
                     PSQPADAAGAAAQDLGRLDPGQLAVQG"
     gene            767..1105
                     /locus_tag="VFX87_03125"
     CDS             767..1105
                     /locus_tag="VFX87_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993928.1"
                     /translation="MPAANRYLRERFLPAFNEEFGRPPADPTSAFVALGRVDLEQILC
                     IEEERVVGRDHVVTADRVALQLAKQPGRRTCAGLRGLVRRHLTGHYSLWYGARCLGRY
                     DRQGRAFHAA"
     gene            complement(1297..1593)
                     /locus_tag="VFX87_03130"
     CDS             complement(1297..1593)
                     /locus_tag="VFX87_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993929.1"
                     /translation="MSPGSRLLPAAALLTLTAAGTGCEYFRDTPEQELANRRWRQCTA
                     ELRDVKLDRVDTDGRIRFTYVTMNERDRALECLEAAGRQGPRLPEPVPAAAAGK"
     gene            complement(1590..2009)
                     /gene="mscL"
                     /locus_tag="VFX87_03135"
     CDS             complement(1590..2009)
                     /gene="mscL"
                     /locus_tag="VFX87_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX4993930.1"
                     /translation="MLKEFKEFAIKGNMMDMAVGIIIGAAFGTVVTSLVSDLLMPVIS
                     AIIGSPDFSNLYVVLRNPTGAVYASVKAARDGGAVVLALGLFINAVISFLIVSAALFF
                     IVKMLNRLRRQEEAAPAAPVPTAEVRLLGEIRDLLRR"
     gene            complement(2035..2814)
                     /locus_tag="VFX87_03140"
     CDS             complement(2035..2814)
                     /locus_tag="VFX87_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX4993931.1"
                     /translation="MLKVGIVGMGVIGHRVAEALTRGIPGTTLVGVSVRNTATAGGYP
                     ALPLPTLIERADLIVEAATQASLREFGPAVLGAGKHLMVLSVGALVGVLDEWARLAEK
                     HGCRILVPSGAIAGLDGVKGAREGAITAVTMETRKPPRGLAGAPYIEQHRIDLDAITT
                     ETLIFEGPAAEAVRAFPANVNVVAALSLAGIGPALTRIKLYAVPGQARNQHRITVEGE
                     FGTLRIEVENVPSENPRTGRLSYLSAIAMLRELGAPVHVGN"
     gene            2901..3785
                     /locus_tag="VFX87_03145"
     CDS             2901..3785
                     /locus_tag="VFX87_03145"
                     /inference="COORDINATES: protein motif:HMM:NF021210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEX4993932.1"
                     /translation="MRSSPRLQEPLDRLYREFDWAARADLDAIRFPIRYADPADREVV
                     ALLSTCMAYGRVDLFGPWVDWALARMGESPARFILGFDPAKHAARFEGFRYRFNRPRD
                     LAAFCLAAQRVLIRHGSLGACFRAGFSPEDPHVGPALERFVGEFLGQDLSAVFPRNRL
                     SYGFRHWFPRPSTGGPCKRLHLFLRWMVRREPPDFGLWTDIPPAALLIPVDTHIENMA
                     RSLGLTRRRSRNWKMAEEITSRLRLLDPADPVKYDFSLCHTRMSGQCLDRRVAAVCGP
                     CRLRPVCRHWRGRRGARA"
     gene            3782..4885
                     /locus_tag="VFX87_03150"
     CDS             3782..4885
                     /locus_tag="VFX87_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX4993933.1"
                     /translation="MSAAWPWAVVAVLAVLVGFTALALWRAVARLGALEAARQQPDQA
                     LLLLQHDIQTARAEAHQARSETLATVKEELHQFASRMTVEMGQVGSGVQQQLQHVGQV
                     VGAVQGSLGKLGEVTQRVFDVGRDIAGLEQLLKSPKIRGGVGETLLENLLAQMLPREQ
                     YMLQHAFATGDRVDAAIRIGERLVPVDAKFPLEGFRRILEETDEERRPALRRAFLRDV
                     KNRVDEIAKKYILPDEGTFDFALMYIPAENIYYEIIVKEDSEEEPPAAYALGRRVVPV
                     SPNTFYAYLRVIVMGLRGLRIERDAQEIQARLGRLRGDLDKFRESFDVVGRHLTNARN
                     KYDDAASGLGRLEGKLEGIERDGDQGALPGIGA"
BASE COUNT          723 a         1844 c         1761 g          771 t
ORIGIN      
        1 ccctggacgg ccagctgacc tggatccagg cggccgagat cctgggccgc agcccccgca
       61 gcatccgccg gctgcgatgg aagctgcaac gcgacggcta tgatgggctg ttcgatcgcc
      121 gccggcaggg gccctcgccc aagcgggccc cggtggccga ggtccaacgc ctgctcgccc
      181 tctaccgcga ccgctaccag gggttcaatg tgcggcactt ccacgagctc gcgcgccggc
      241 agcacggcgt cggcttctgc tccgccttcg tcaagaaggc cctgcagagt gcgggtctcg
      301 tccccaaaca ccgcccccgc ggccgtcacc ggcgcgcgcc gcgcccgtgc ttcggcgaac
      361 tgctgcacct cgatggcagc cgccatcgct ggctggccct ggtccccgac cagtggttca
      421 ccctcatcgc cgtcgtcgac gacgccacca agcgcttgct ctatgccgag ctctgggaca
      481 gcggggaaag cgtcggggcc atcatgaccg cgctgcgggc ggtcctcgag cgctacggcc
      541 tgcccatggc cctctccacc gaccgcgccc actgggccgt gcacaccccg gtggccggtg
      601 gcgccccgga tcgccgcgcc cgactcaggt cggccgtgcc ctcgcccgcc tggggatcga
      661 acatatcctc ggctactcgc cggcggcccg cggccgtagt gagcgcgtca accgcaccct
      721 gcaagatcgc ctcgtcaacg agctccgcct ggctgggctc gccacggtgc ctgcggccaa
      781 ccgctacctg cgcgagcggt tcctgccggc gttcaatgag gagttcgggc ggccgcccgc
      841 ggatcccacg tccgccttcg tggcgctcgg ccgcgtcgat ctcgagcaga tcctctgcat
      901 cgaggaggag cgcgtcgtcg gccgcgacca tgtcgtcacg gcggatcgcg tggccctgca
      961 gctggccaag cagcccggcc gccgcacctg cgcgggcctc cgcggcctcg tccgccgcca
     1021 tctcaccggc cactactccc tgtggtacgg cgcccggtgc ctggggcgct atgaccggca
     1081 gggccgcgcc ttccacgcgg cctgaccccc cttgcggtgc acccgagcgg tcatgtcacg
     1141 gtgtcaaaca gttgcggtca gatcacgtat cagcaacagc gggaagcagg taacgcgagg
     1201 ccgtgaccac ggagggtgcc ggagacatgg cggccctgga ggccgaccgg tatctcgagc
     1261 cgaacgggct caaatagcag ggcgaagcgc agccgattac ttgcccgccg cggctgccgg
     1321 aaccggctcg gggagccgcg gcccctgccg gcctgccgcc tcgaggcatt cgagcgcccg
     1381 gtcgcgctcg ttcatcgtca cgtaggtgaa gcggatgcgc ccgtcggtat ccacccgatc
     1441 gagcttaacg tcgcggagtt ccgcggtgca ttgccgccag cgccggttcg ccagctcttg
     1501 ctccggggta tcacggaaat actcgcagcc cgttccggct gccgtcagcg tgagcagggc
     1561 ggcagccggg agcagccgag agccggggct caccgccgga gaaggtctcg gatctcgccc
     1621 aacagccgta cctccgcggt gggcacgggc gcggcgggcg ccgcctcctc ctgtcgccgc
     1681 agccggttga gcatcttcac gatgaagaac aacgccgccg acacgatgag gaacgagatc
     1741 accgcgttga tgaagaggcc gagggccagc accaccgcgc ccccgtcccg ggccgccttc
     1801 accgacgcgt acacggcgcc ggtcggattg cgcagcacca cgtagaggtt gctgaagtcg
     1861 gggctgccga tgatggccga gatcaccggc atcagcaggt cgctgacgag cgaggtgacc
     1921 accgttccga aggccgcgcc gatgatgatg ccgacggcca tgtccatcat gttgcccttg
     1981 attgcgaact ccttgaactc cttcagcatg cgctcgcctc ctcctctttc agggtcagtt
     2041 cccaacgtgg acgggagcgc ccagctcccg cagcatcgcg atcgcggaca ggtacgagag
     2101 ccggccggtc cgcgggttct ccgacggcac gttctcgacc tcgatgcgca gggtgccgaa
     2161 ctcgccctcg accgtgatcc ggtgctggtt gcgcgcctgt cccggcaccg cgtagagctt
     2221 gatgcgcgtg agcgccggcc cgatgccggc cagggacagg gcggccacca cgttgacgtt
     2281 ggccgggaag gctcggaccg cctcggcggc cggcccctcg aagatcagcg tctcggtggt
     2341 gatggcgtcg aggtcgatgc ggtgctgctc gatgtacggg gcgccggcca gcccgcgcgg
     2401 cggcttccgc gtctccatgg tgaccgcggt gatcgcgcct tcgcgcgcgc ccttcacgcc
     2461 atcgaggccc gcgatggcgc cggagggcac caagatgcgg cagccgtgct tctcggccag
     2521 ccgcgcccac tcgtccagca cgcccacgag ggcgccgacc gagaggacca tcaggtgctt
     2581 gcccgctccg agcaccgcgg ggccgaactc gcgcagcgag gcctgggtgg cggcctccac
     2641 gatcaggtcc gcgcgctcga tgagcgtcgg cagcggtagc gcggggtagc ccccggcggt
     2701 cgccgtgttc cgcacgctga cacccaccag cgtggttccg gggatgcctc gggtcagcgc
     2761 ctccgccacg cggtggccga tgactcccat gccgacgatt ccgaccttga gcataatgcg
     2821 gcgtcctttc cccccgagag tgtggcgatg atagcgccgc cccccgacgg ccacaaggga
     2881 cgtgctagaa tcagccgcgg atgcgttcat cgccccggct ccaggagccg ctcgaccggc
     2941 tctaccgcga gttcgactgg gccgcccgcg ccgacctgga cgccatccgt ttcccgatcc
     3001 gctacgccga ccccgccgac cgcgaggtgg tcgcgctgct gtccacctgc atggcctacg
     3061 gtcgggtgga cctcttcggc ccctgggtcg actgggccct tgcccgcatg ggggagtcgc
     3121 ccgcgcgctt catcctgggc ttcgatccgg ccaagcacgc cgcgcgcttc gagggcttcc
     3181 gctaccgctt caatcgcccg cgcgatctcg ccgccttctg cctggccgcc cagcgcgtgc
     3241 tgatccgcca cggctcgctc ggcgcctgct tccgcgccgg cttctctccg gaggacccgc
     3301 acgtgggccc ggccctcgag cgcttcgtcg gcgagttcct cggccaggat ctctcggcgg
     3361 tgttcccgcg caaccggctc tcctacgggt tccggcactg gtttccgcgc ccgtccaccg
     3421 gcgggccgtg caagcgcctg cacctcttcc tgcgctggat ggtgcgccgg gagccgccgg
     3481 acttcggcct ctggaccgac attcctcccg ccgcgctgct gatcccggtg gacacgcaca
     3541 tcgagaacat ggcgcgatcg ctggggctca cgcgccggcg cagccggaac tggaagatgg
     3601 ccgaggagat tacgtccaga ctgcgcttgc ttgatccggc cgacccggtg aagtacgact
     3661 tctcgctctg ccacacgcgg atgtcgggcc agtgcctcga ccggcgcgtg gcggcggtgt
     3721 gcgggccctg ccgtctccgg ccggtgtgcc gccactggcg cggtcggcgc ggagcgcgcg
     3781 cgtgagcgcc gcgtggccgt gggccgtcgt cgcggtgctg gcggtgctgg tcggattcac
     3841 cgccctggcc ctgtggcgcg cggtggcgcg gctcggggcg ctcgaggcgg cccgccagca
     3901 gccggatcag gccctgctgc tcctgcagca cgatatccag accgcacgcg ccgaggccca
     3961 ccaggcccgg agcgagacac tggccaccgt gaaggaggag ctgcaccagt tcgcgagccg
     4021 gatgacggtg gagatggggc aggtgggcag cggcgtgcag cagcagctcc agcacgtggg
     4081 ccaggtggtc ggcgcggtgc agggcagcct cggcaagctc ggcgaggtga cccagcgggt
     4141 cttcgacgtg gggcgcgaca tcgcgggcct cgagcaattg ctcaagtcgc cgaagatccg
     4201 gggcggggtc ggcgagaccc tgctcgagaa cctgctggcc cagatgctgc cgcgggagca
     4261 gtacatgctg cagcacgcgt tcgccaccgg ggaccgcgtg gacgcggcca tccggatcgg
     4321 cgagcggctc gtgcccgtgg acgccaagtt cccgctcgag ggcttccgcc ggatcctgga
     4381 ggagacggac gaggagcggc ggcccgccct gcgccgcgca ttcctgcgcg acgtcaagaa
     4441 ccgggtggac gagatcgcca agaaatacat cctgccggac gagggcacct tcgacttcgc
     4501 cctgatgtac atcccggccg agaacatcta ctacgagatc atcgtgaagg aggacagcga
     4561 ggaggagccg cccgcggcct acgccctggg gcgccgggtg gtgccggtct cacccaacac
     4621 cttctatgcc tacctgcggg tgatcgtgat ggggctccgg ggcctccgga tcgagcgcga
     4681 cgcccaggag atccaggccc ggctgggccg cctgcggggg gatctggaca agttccggga
     4741 gtccttcgac gtggtcggcc ggcacctgac caatgctcgg aacaagtacg atgacgcggc
     4801 ctccgggctg gggcggctcg agggcaagct ggaggggatc gagcgcgatg gcgaccaggg
     4861 ggcgctgccc ggaatcggcg cgtgatcggg cacttggccg agcccgcccc tggtccattt
     4921 gggaacaatc gcttgtggta gtatcgaaga aatccaccat cagagggatc catgcctgat
     4981 atccagttcc aggtgtacgg catcaacctc ctcgtgcggc tcatggtcga gcccccggcg
     5041 gacgtgcgcg tgcactgccc gaagggctcg ccgatccgct acggcgaggt ggtgggccg
//
LOCUS       DASUFA010000080         6519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_237674, whole genome shotgun sequence.
ACCESSION   DASUFA010000080 DASUFA010000000
VERSION     DASUFA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237674"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFX87_03155"
     CDS             complement(<1..473)
                     /locus_tag="VFX87_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX4993934.1"
                     /translation="MPLTTQEIEALKKITSPSIANAIETFKVRPREQGNLSSDIRALF
                     REMGPMVGYAVPCVIRAEQGPIEGHRASTYGWWDFVLTIPAPRVIVVHDLDDPRGQGA
                     QWGEVQANIHRALGCVGVVTDGSVRDLDEVRALGFQFCAAHVSVSHANVHMVDFG"
     gene            complement(509..1171)
                     /locus_tag="VFX87_03160"
     CDS             complement(509..1171)
                     /locus_tag="VFX87_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4993935.1"
                     /translation="MSRPVAGLAVLALALGLLVAGAAGQAAAPLTRERALADITSPDL
                     DPRRRAAAWLGEVGASADAPVLIEALSDADEVVRVLAERSIWQVWSRSGDAEADALLQ
                     VGIEQMSRGDGPAAAETFTKVIQRRPDFAEAWNKRATVYFLMGEDEKSLRDCDEVMKR
                     NPAHFGALAGYGQIYLRLDQPERALSYFRRALRINPNLRGVQQVIPQLEQLLTERRKG
                     TI"
     gene            complement(1168..3180)
                     /gene="ligA"
                     /locus_tag="VFX87_03165"
     CDS             complement(1168..3180)
                     /gene="ligA"
                     /locus_tag="VFX87_03165"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX4993936.1"
                     /translation="MPSPADVSARLDELREQINRHNYLYYTENRPEVSDAEYDRLWRE
                     LVALEQAHPELITPDSPTQQPGGRRAETFAPVEHLVAMLSLDNAMTAEELHEFEARIK
                     RAVPGITPAYVCEPKIDGLGVALLYERGRFVRGATRGDGRVGEDITQHLRTIKSIPPT
                     LVGPLKGAKRLEVRGEVYMPREAFARLNAGLEEAGQPVFANPRNAAAGAVRQKDPAVT
                     ASRPLDILLYHVSVLEPPAFHSQWERLEALRGSGFPVSPRSERGRTMDEVLAYCIRIE
                     AERDTLAYDADGVVVKVDDLEQQRRLGATAHHPRWAIAYKFAARQATTRVLGITINVG
                     KTGALTPTAQLEPVELAGVTVSNVSLHNEDEVRRKDVRVGDTVLIERAGDVIPYLVQV
                     VTARRPPDATPFRMPSHCPACGGLAARAEGEAVMRCTNIACPAQLKERLFHWGSRRAM
                     DIEGLGEVIIGQLVDRGLVRDFADLYGLDVDTLAELERMAEKSARNLHRAIEGSRTRG
                     LSRLLNALGIRMVGERAAQLLAARFGTMERLLAATEADINEIYGIGPQIAQAVTSFLT
                     EPRNRATIERLAAAGVVMTEQGHTEGPRPLEGKTLVLTGGLASLSRDQAKDLILRLGG
                     RVTGSVSKKTDYVVVGEDAGSKADDARRLGVATLDEAAFLMLVGRA"
     gene            complement(3264..5357)
                     /gene="gspD"
                     /locus_tag="VFX87_03170"
     CDS             complement(3264..5357)
                     /gene="gspD"
                     /locus_tag="VFX87_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX4993937.1"
                     /translation="MRAPGRQLVLNFDNADVEVVVQAAAEIVGFNYVLAPGARGRKVT
                     VQTSGKISSDEVFGVLLTILDVNGLAAVRSGNLYRIIPREGVAQTPVKTVVGREVGGG
                     LPPDEMVTQIVPLRFINAQDAVTLLRPFVPAQGAITAHRETNLLILTDTNANIRRLLD
                     MLQIVDVEVALSELQIIALRHADAQELAQLLAQLFVTRQGAGALGAAAPPPPPAPPGV
                     PAVPGAGPPGAPGSERPMIVPERRSNSLVVHARKPDMETIRRLIEKLDVDIYGGQRVF
                     IYFAENTKARDLATTLDAIYGRGDRGPAITGTQQPPIGSASISGPYGAYPSRISSPAL
                     LPTPLPPTPAPIGTLAPRAAGAPTAFPGVSGEGAPPGIEIRFVADEVTNAVIVTTYPR
                     LWKEIEETIKKLDKMPRQVLIEVLAAEVTLTDDTKLGIEWAVRSGNVTISSSPSGLLP
                     GRPSQGLIPLGGALPLGFNVFAFAADKFLAALNALASDNKVNVLSNPSILTAENRKAV
                     INVSTSVPIVTSQQVPVATGGTTGNSITQTVEYKDAGIILTVTPRIGEQGTVALDVKQ
                     EFNEVGENEPPPINSPRFRKREAETSVVLANSQTLILGGLIQNRRTRIQIGVPLLNRI
                     PILGYLFGSVEEKIEKTELILLITPRVIGTALDAARITEQMRKATPELDQSIRQAPRQ
                     PAAAPPPPPPPPPAQ"
     gene            complement(5687..6313)
                     /locus_tag="VFX87_03175"
     CDS             complement(5687..6313)
                     /locus_tag="VFX87_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993938.1"
                     /translation="MARRWLILDILIGAVGCLLAAGLIRELSTARPLPAPPAARPAPS
                     TPVSAPAPASQQGAQIYGGIATKNLFHPGRSEALAGPVVATGSKPLLHGLVMDGPKSR
                     AYLEDPVAKRTFGYAVGDTVGSGRLQSISHDRVVIAGPDGPTEVLLQDASKPKPVATP
                     GLAGAPSQVPPVATAAPVTPTQPGAGAPAASPFQALPGSTTAPRARDR"
     gene            complement(6318..>6519)
                     /locus_tag="VFX87_03180"
     CDS             complement(6318..>6519)
                     /locus_tag="VFX87_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993939.1"
                     /translation="SIRDVVAALARLERTGCLLALKDVKIRVVAVGQPRELLTTLTVA
                     GYLLPGAAPAKAEARPARPGEG"
BASE COUNT          868 a         2193 c         2461 g          997 t
ORIGIN      
        1 atcccgaagt ccaccatgtg cacgttggcg tgcgagaccg agacgtgggc cgcgcagaac
       61 tggaagccga gcgcgcgcac ctcgtcgaga tcacgcaccg agccgtcggt gaccacgccc
      121 acgcagccca gcgcgcgatg gatgttcgcc tggacctcgc cccactgggc gccctggccg
      181 cgcgggtcgt cgaggtcatg caccacgatc acgcgcggcg cggggatcgt caggacgaag
      241 tcccaccagc cgtaggtgct ggcccggtgg ccctcgatgg gcccctgctc ggcccggatc
      301 acgcagggga cggcatagcc caccatcggc cccatctcgc ggaaaagcgc gcggatgtcg
      361 gaggagaggt tgccctgctc tcggggccgc accttgaagg tctcgatggc gttggcgatg
      421 ctggggctgg tgatcttctt gagggcttcg atctcttggg tggtgagcgg cacgatgtcc
      481 tccgtgtggg gttgacggct cggtccggtc agatagtgcc ctttcgccgc tccgtgagca
      541 actgctcgag ctgggggatg acctgctgga caccgcggag gttgggattg atgcgcagcg
      601 cgcggcggaa gtaggacagg gcgcgctcgg gctggtccag gcgcaggtag atctgcccgt
      661 agccggcgag ggcgccgaag tgggccgggt tgcgcttcat cacctcgtcg cagtcccgga
      721 gcgacttctc atcctcgccc atcaggaagt acacggtggc gcgcttgttc caggcctccg
      781 cgaagtcggg ccggcgctgg atcaccttgg tgaacgtctc cgccgcggcg gggccgtcgc
      841 cccggctcat ctgctcgatc ccgacctgga ggagcgcgtc ggcctccgcg tcgccggacc
      901 tgctccagac ctgccagatc gagcgctccg cgagcacgcg gaccacctcg tcggcgtcgg
      961 agagcgcctc gatcaggacg ggcgcgtccg cgcttgcacc gacctccccc agccaggcgg
     1021 cggcgcgccg gcgcgggtcg agatcggggc tcgtgatgtc ggccagggcc cgctcgcggg
     1081 tgaggggggc ggcggcctgg cccgccgcgc cggcgacgag caggcccagc gccagcgcga
     1141 ggacggccag cccggcgacc gggcggctca cgcgcgcccc accagcatca ggaacgcggc
     1201 ctcgtcgagc gtggcgacgc ccaggcgccg ggcgtcgtcg gccttggatc ccgcgtcctc
     1261 gccgacgacc acgtagtccg tcttcttcga caccgagccg gtgacccggc cgcccaggcg
     1321 gaggatcagg tccttggcct ggtctcggct caggctcgcg aggccgccgg tcagcaccag
     1381 ggtcttgccc tcgagcggcc ggggcccttc cgtgtggccc tgctcggtca tgaccacgcc
     1441 cgccgcggcg agacgctcga tggtggcccg gttgcgcggc tccgtgagga agctcgtcac
     1501 cgcctgggcg atctgggggc cgatgccgta gatctcgttg atgtcggcct cggtggcggc
     1561 gaggaggcgc tccatggtgc cgaagcgcgc ggccaggagc tgcgcggccc gctcgcccac
     1621 catgcgaatg ccgagggcgt tgagcagccg cgagaggccg cgtgtcctcg agccctcgat
     1681 cgcgcgatgg aggttcctgg ccgacttctc ggccatgcgc tccagctcgg cgagggtgtc
     1741 cacgtcgagc ccgtagagat cggcgaagtc ccggaccagc ccgcgatcca cgagctggcc
     1801 gatgatcacc tccccgaggc cctcgatgtc catcgcccgc cgcgagcccc agtgaaagag
     1861 ccgctccttg agctgggcgg ggcaggcgat gttggtgcag cgcatcaccg cctcgccctc
     1921 ggcacgggcg gccaggcccc cgcaagccgg gcagtgggag ggcatcctga acggggtggc
     1981 gtccggcggc cgccgggcgg tcaccacctg gaccagatag gggatgacgt cgcccgcccg
     2041 ctcgatcagc acggtgtcgc ccacgcgcac gtccttgcgg cggacctcgt cctcgttgtg
     2101 gagactcacg ttggagacgg tcacgccggc cagctccacc ggctcgagct gcgcggtggg
     2161 ggtgagtgcg ccggtcttgc ccacgttgat cgtgatgccg agcacccgcg tggtggcctg
     2221 gcgcgccgcg aacttgtagg cgatggccca gcgcgggtgg tgcgcggtgg cgccgagccg
     2281 gcgctgctgc tcgagatcgt ccaccttgac cacgacgccg tcggcgtcgt aggcgagcgt
     2341 gtcgcgctcg gcctcgatcc ggatgcagta ggccaggacc tcgtccatgg tgcggccccg
     2401 ctcggagcgc gggctcaccg ggaagccgct cccccggagc gcttcgagcc gctcccactg
     2461 ggagtggaag gcaggcggct cgagcacgct cacgtggtag aggaggatgt cgagggggcg
     2521 cgacgcggtc accgccgggt ccttctgccg caccgccccc gccgccgcgt tgcgcgggtt
     2581 ggcgaagacg ggctggccgg cctcctcgag ccccgcgttg agccgggcaa aagcctcgcg
     2641 cggcatgtag acctcgcccc gcacctcgag ccgcttcgcg cccttcaggg gcccgaccag
     2701 cgtaggcggg atggacttga tcgtgcgcag gtgctgggtg atgtcctcac cgacacgtcc
     2761 gtcgccccgc gtggcccccc gcacgaagcg cccgcgctcg tagagcagcg ccaccccgag
     2821 gccgtcgatc ttgggctcgc agacgtacgc gggggtgatg ccgggcaccg cccgcttgat
     2881 ccgcgcctcg aactcgtgga gctcctcggc ggtcatcgcg ttgtcgagcg agagcatggc
     2941 caccaggtgc tcgacgggcg cgaaggtctc cgcccggcgg ccccccggct gctgggtggg
     3001 gctgtccggc gtgatcagct ccgggtgggc ctgctcgagc gccaccagct cgcgccagag
     3061 gcggtcgtac tcggcgtcgc tgacctcggg gcggttctcc gtgtagtaga ggtaattgtg
     3121 gcggttgatc tgctcgcgca gctcgtcgag ccgtgcgctg acgtcggccg gggagggcat
     3181 gacgccttcg aggatagcaa ccgccctgag ggcgcgccag cgagccctct ccctggcgaa
     3241 gagggaggtg aggggccgtt gccctactga gccgggggcg gggggggggg cggcggcgga
     3301 gccgccgcag gctgacgagg cgcctggcgg atcgactggt ccagctccgg cgtcgccttc
     3361 cgcatctgct cggtgatccg cgcggcatcg agggcggttc cgatcacccg gggcgtgatc
     3421 aggaggatca gctcggtctt ctcgatcttc tcctcgaccg agccgaagag ataccccagg
     3481 attggaatgc ggttcaggag gggaacgccg atctggatgc gggtgcgccg gttctggatg
     3541 agcccgccga ggatcagggt ctgggagttg gcgagcacga ccgaggtctc ggcttcgcgc
     3601 ttcctgaagc gcggggagtt gatcgggggc ggctcgttct cgccgacctc gttgaattct
     3661 tgcttgacgt cgagggcgac ggtgccctgc tccccgatgc gcggggtgac ggtcaggatg
     3721 atgccggcgt ccttgtactc cacagtctgg gtgatggagt tgccagtggt gccgccggtg
     3781 gccaccggca cctgctggga ggtcacgatg gggacggagg tcgagacatt gatgacggcc
     3841 ttccggttct cggcggtcag gatggacggg ttcgacagca cgttgacctt gttgtcgctg
     3901 gccagcgcgt tgagcgcggc caggaacttg tctgccgcaa acgcgaagac gttgaagccg
     3961 agcggtagcg cgcccccgag cgggatgagg ccctggctcg ggcggccggg aagtagcccc
     4021 gacggcgagg aggagatcgt gacattcccc gaccggacgg cccactcgat acccagcttg
     4081 gtgtcgtcgg tgagcgtgac ctccgccgcg agcacttcga tcagcacctg acggggcatc
     4141 ttgtcgagct tcttgatcgt ctcctcgatc tccttccaga gccgcgggta cgtggtgacg
     4201 atgaccgcgt tggtgacctc gtcggcgacg aagcgaatct cgatgccggg cggcgcaccc
     4261 tcccccgaga cgcccgggaa ggcggtcggc gcgcccgcgg cccgcggcgc gagcgtcccg
     4321 atcggcgccg gggtcggcgg cagcggcgtc ggaaggagtg cgggcgagga gatccggctc
     4381 ggataggccc cgtacggtcc ggagatgctg gcggaaccga tcggcggttg ctgggtcccg
     4441 gtgatcgcgg gcccgcggtc gccccggccg tagatggcgt cgagcgtcgt ggccaggtct
     4501 cgggccttcg tgttctccgc gaagtagatg aagactcgct ggccgccgta gatgtccacg
     4561 tccagcttct cgatcaaccg gcggatcgtc tccatgtccg gcttccgggc gtggacgacc
     4621 agcgagttgg agcggcgctc ggggacgatc atcggccgct cgctccccgg ggccccgggc
     4681 ggacccgcgc cggggacagc cgggacgccg ggcggggcag gcggtggtgg cggggccgcc
     4741 gcgccgagcg ctcccgcgcc ctggcgagtg acgaagagct gcgcgagcag ctgggccagc
     4801 tcctgggcat ctgcgtgcct gagagcgatg atctgcagct cgctgagggc cacctccaca
     4861 tccacgatct gcagcatgtc gagcagccgg cggatattgg cgttcgtgtc ggtcaggatc
     4921 agcagattcg tctcgcgatg ggcggtgatg gcgccctgcg cagggacgaa ggggcgcagc
     4981 agcgtcaccg cgtcctgggc attgatgaac cgcagcggca cgatctgcgt caccatctcg
     5041 tcgggcggga gcccgccgcc gacttcccgg cccacgaccg tcttcaccgg ggtctgggct
     5101 accccctcgc gcgggataat acggtagaga ttgcccgagc gcactgcggc cagtccgttg
     5161 acatcgagga tcgtgagcaa gaccccgaag acctcgtcgc tcgagatctt tccactggtt
     5221 tgcaccgtga ctttgcggcc gcgggcaccg ggagcgagca cgtagttgaa gccgacgatc
     5281 tcggccgcag cctggaccac cacctcgacg tcggcgttgt cgaagttgag cacgagctgc
     5341 cggccgggcg cacgcacggc gggcgccggc tcgtccggca cattcacggt cacaccggga
     5401 gggggaccgc cgggcccggg tgtcgccgcg ggcggcggtg ctgcctcgtg cttctcggct
     5461 ggaccgaccg acagggcttc ggcggacgtg gtggtgcccg gcgggcgtgg cggggcgctc
     5521 ggtgtgggag ccggctcggt ggtgacctcg acccgggtac ggggaggctc gcccggccga
     5581 ccttcgctcg tcgcgtcgcg cggctgcggc cggccggcga cgcagccggc cgtcgcccag
     5641 atcagcatta ggacaaggat cccgcttgcg gctcggcgtg ggtcgctcat cggtccctgg
     5701 ctcgcggcgc ggtggtggag ccgggcagcg cttgaaaggg agacgcggcg ggcgctccgg
     5761 cccccggctg ggtgggcgtg actggcgcag cggtggctac cggcgggacc tggctgggcg
     5821 cgccggccag gcccggtgtg gccacgggct tgggcttcga cgcatcctgc agcagcacct
     5881 cggtcggccc gtcagggccg gcgatcacga cgcggtcgtg gctgatggac tgcaggcggc
     5941 cgctgccgac ggtatcgccg actgcatagc cgaatgtgcg cttcgccacg ggatcttcga
     6001 ggtaggcgcg gctcttcggg ccgtccatca cgaggccgtg gagtagcggc ttggagccgg
     6061 tggccacgac gggcccggcg agggcttcgg accggccggg gtggaagaga ttcttggtcg
     6121 cgatgccgcc gtagatctgg gctccctgct gcgacgccgg agccggagcg gagacgggcg
     6181 tcgacggcgc agggcgtgct gcgggtggag ccggcagcgg gcgtgcggtg ctcagctcac
     6241 ggatcagccc ggcggcgagg aggcagccga ccgcgcctat caggatatcg aggataagcc
     6301 accgtctggc cacgggctca gccttccccg ggtcgggcgg gcctggcttc ggccttggcc
     6361 ggcgccgccc cgggcaggag atagccggcg acggtgagcg tggtcagcag ctcccgaggc
     6421 tggcccaccg cgaccacccg gatcttgacg tccttgagcg cgagcaggca gccggtgcgc
     6481 tcgagacgcg cgagcgcggc gaccacgtcg cggatgctc
//
LOCUS       DASUFA010000081         4471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_133226, whole genome shotgun sequence.
ACCESSION   DASUFA010000081 DASUFA010000000
VERSION     DASUFA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133226"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VFX87_03185"
     CDS             <1..1378
                     /locus_tag="VFX87_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX4993940.1"
                     /translation="RRLANEAAAEEARSRGPLREETVRLSYADPEEVAKTLQGILGIP
                     PEGTAPVTGTLPSVPAVISSTAPNLALPVQPPFSALYGPGQPPAQAVSISQDVLAKGI
                     TIRSHKPTNTIFIRHYQADLERIKKLIHEQLDIPLPQVKIEARMEILDRTALEAIGVQ
                     WGGATAGNVGSTTLVGQGFQSAPSTVPGLTLPVVGGVLQPDGSTIVTGPTSPQGFQPF
                     NQSMTLSRLLPIDRTTGLPIGGNLVNIPFQSLPNASNFTPAGGIAFGIVGTKFNINLA
                     LQALENQGKTRTLARPEIVTVENNKAEVSLGEEIPYATVSSAGTQIQFKEAVLKLTVT
                     PTVTREKLNNEEITKIKMVVIVENNSRGDTITPAAGVSVPIINRRKAETQVLIREGER
                     LVIGGVTQSVQQNTVRKVPLFGDIPLLGWLFKARETFESGRELVVFLTPSLVKSDGAL
                     AARPASGR"
     gene            1387..2559
                     /gene="aroC"
                     /locus_tag="VFX87_03190"
     CDS             1387..2559
                     /gene="aroC"
                     /locus_tag="VFX87_03190"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX4993941.1"
                     /translation="MGAAFRFLTAGESHGEALTAVIEGLPAGLPLTEGDINEDLARRQ
                     RGYGRGGRMKIERDEVHISAGVRGGLTLGSPVTLTVQNRDWENWKQTMAVGDSPAGAS
                     PKTVTRPRPGHADLAGAMKYNHRDIRNVLERSSARETTARVAVAGVARRFLREFGITI
                     MSHVTEIGGVHIGPLALPWDEIARRAEASEVRCADPEAERLMIEAIDAAKGKGDTLGG
                     VFEVVAIGCPVGLGSYVHWDRKLDGRLAQAFCSIHAIKGAEMGMGFEAARRPGSEVHD
                     EILLDAAGGFTRGTNSAGGLEGGVTNGQPVIVRAAMKPISTLRRPLRSVDIQNKEAVE
                     AVVERSDVVAVPAAGVVGEAMMAIVLAEAFLEKFGGDSLDEIRRNYQTYRDYLKSW"
     gene            2598..3680
                     /gene="aroB"
                     /locus_tag="VFX87_03195"
     CDS             2598..3680
                     /gene="aroB"
                     /locus_tag="VFX87_03195"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX4993942.1"
                     /translation="MHDISVKLGPRSYRIVVDAGILGRVGPELRALGVGGKVALFSDA
                     GILARLGGPVRGSLAAAGFEVAVVELPAGEAAKSVEVASRGWDALLAAGLDRGSTVVA
                     LGGGAVGDLAGFVAATYMRGVNFVQLPTTLLAQVDASIGGKTAIDHPRAKNLVGAFHQ
                     PRLVLVDPAALTSLPEREFRSGLAEVVKHGIVLDADYFADLEKSLVALLGRDIETLTR
                     VVAGSCRLKANVVERDEQEAELRAVLNYGHTIGHAIEAVTGYARWTHGEAVSLGIAAE
                     ARLAERLGVASRETAARQIDLLRAVGLPVRDSAASPRAVLEALGRDKKAREGRVPFVL
                     APAIGRFTVVYDVPREAVLETLQELG"
     gene            3677..>4471
                     /locus_tag="VFX87_03200"
     CDS             3677..>4471
                     /locus_tag="VFX87_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993943.1"
                     /translation="MIQRGSGDPHRAGPSFEADPRLIDLGVALFLVLVTVLTFLPGLE
                     GAFLDWDDRENFVHNPHYRGLGPAQLRWMLTTGLLGHWMPLTWLSLAVDWTIWGMDPF
                     GYHLTSLGLHAICAVLVYALARRLFSLAAPSVPRTTHVLGAAVAALLFAIHPLRVESV
                     AWITERRDVLAGVFFLGAVIAYLGRWDPDGTARPRPRLRYLAVLALFAAALLSKAMSV
                     SLPVVLLILDVYPLCRLGGSPRGWLRKDARPVWLEKIPLVVLSVGAT"
BASE COUNT          655 a         1616 c         1545 g          655 t
ORIGIN      
        1 ccggcggctt gccaacgagg cggccgcgga agaggcgcgc tcgcgcggac ccctccgcga
       61 ggagaccgtg cgcctctcct atgccgaccc ggaggaggtt gccaagaccc ttcagggcat
      121 cctgggcatt ccgccggagg gcaccgcgcc ggtgaccggc acccttccca gcgtgccggc
      181 cgtgatcagc agcaccgcgc cgaacctcgc cctgcccgtc cagccgccct tctcggcgct
      241 gtatggaccc ggtcagcccc ccgcccaggc ggtgtccatc agccaggacg tgctggccaa
      301 gggcatcacc atccggtcgc acaagcccac caacaccatc ttcatccgcc actaccaggc
      361 cgatctcgag cggatcaaga agctgatcca cgagcagctc gacatcccgc tgccgcaggt
      421 caagatcgaa gcccgcatgg agatcctgga ccgcaccgcg ctggaggcga tcggcgtgca
      481 gtggggtggc gccaccgccg gcaatgtggg ctccaccacc ctcgtgggcc agggcttcca
      541 gtccgcgccg agcacggtac ccggcctgac cctgcccgtg gtgggcggcg tgctccagcc
      601 ggacggctcc accatcgtga ccggcccgac ctccccccag gggttccagc cgttcaacca
      661 gtccatgacg ctcagccggc tcctgcccat cgaccgcacc accggcctgc cgatcggcgg
      721 caacctggtc aatatcccct tccagagcct gcccaacgcc tcgaacttca cgcccgccgg
      781 cggcatcgcg ttcggcatcg tgggcaccaa gttcaacatc aacctggcgc tgcaggcgct
      841 ggagaaccag ggcaagacgc ggaccctggc ccggccggag atcgtgaccg tcgagaacaa
      901 caaggccgag gtgtccctcg gtgaagaaat cccgtacgcc accgtcagct cggcgggtac
      961 ccagatccag ttcaaggaag cggtgctgaa gctcaccgtg acaccgacgg tgacccgtga
     1021 gaagctgaac aacgaggaga tcacgaagat caagatggtg gtgatcgtcg agaacaattc
     1081 ccgcggtgac accatcacac cggcagccgg cgtcagcgtg ccgatcatca accgccgcaa
     1141 ggcggagacc caggtgctca tacgggaagg cgagcgcctc gtgatcggcg gcgtgaccca
     1201 gtcggtgcag cagaataccg tccggaaggt cccgctattc ggcgacatcc cgctgctggg
     1261 ctggctcttc aaggcgcggg agaccttcga gtcgggacgc gagctggtgg tgttcctcac
     1321 gccttcgctc gtgaagtcgg acggcgcgct ggcggctcgg cccgcgtccg gcagatagcg
     1381 gcggcggtgg gcgcggcgtt ccgcttcctc accgcgggcg agtcccacgg agaggcgctg
     1441 accgccgtca tcgaggggct gcccgcgggc ctcccgctca ccgaggggga catcaacgag
     1501 gacctcgcgc gccgccagcg cggctacggc cgcggcgggc ggatgaagat cgagcgtgac
     1561 gaggtccaca tctccgccgg cgtgcgcggg ggactcaccc tcggcagtcc ggtcacgctg
     1621 accgtccaga accgcgactg ggaaaactgg aagcagacca tggcggtggg cgactcgccg
     1681 gcgggcgcct cccccaagac ggtcacccgc ccccgccccg gccacgccga cctggccggg
     1741 gccatgaagt acaaccaccg cgacatccgc aatgtgctgg agcgctccag cgcccgcgag
     1801 accaccgcgc gggtggcggt ggcgggcgtg gcgcggcggt tcctccggga gttcggcatc
     1861 accatcatga gccacgtcac cgagatcggc ggcgtgcaca tcggccccct cgcgctgccc
     1921 tgggacgaga tcgcgcggcg cgcggaggcc agcgaggtcc gctgcgccga tcccgaggcg
     1981 gagcggctca tgatcgaggc catcgacgcc gccaagggca agggcgacac cttgggcggg
     2041 gtattcgagg tggtggccat cggctgcccg gtgggactcg ggtcctacgt gcactgggat
     2101 cgcaagctcg acggccggct cgcgcaggcc ttctgctcca tccacgccat caagggcgcg
     2161 gagatgggca tgggcttcga ggcggcgcgc cggccgggct cggaggtgca cgacgagatc
     2221 ctcctcgatg ccgcgggggg cttcacccgg ggcaccaaca gcgcgggcgg tctcgagggt
     2281 ggggtcacca atggccagcc ggtgatcgtg cgcgcggcca tgaagcccat ctcgaccctc
     2341 cgccggcccc tccgctcggt ggacatccag aacaaggaag cggtagaggc cgtggtggag
     2401 cgcagcgacg tggtggcggt gccggcggcg ggtgtcgtcg gcgaggcgat gatggccatc
     2461 gtgctggccg aggcctttct cgagaagttc ggcggcgaca gcctcgacga gatccgacgc
     2521 aactaccaga cgtaccggga ctacctcaag agctggtaac gcggcgcccc ccggcgcggc
     2581 cgggtacact actctccatg cacgacatct cggtgaagct cggcccgcga tcctatcgga
     2641 tcgtcgtgga cgcgggaata ctcgggcgtg tcggccccga gctcagggcc ctgggcgtcg
     2701 gcggcaaggt cgcgctcttc tccgacgccg gcatcctggc ccgcctgggc ggccccgtcc
     2761 gcggcagcct cgccgccgcg ggcttcgagg tcgcggtggt cgagctgccc gcgggcgagg
     2821 cggccaagag cgtggaggtg gcgagccggg gatgggacgc cctgctggcc gccggcctgg
     2881 accgcggatc caccgtggtg gcgctggggg gcggcgcggt gggcgatctc gcgggcttcg
     2941 ttgccgccac ctacatgcgc ggcgtcaact tcgtccagct ccccacgacg ctgctcgctc
     3001 aggtggacgc gtccatcggc ggcaagaccg ccatcgacca tccccgggcc aagaacctcg
     3061 tcggcgcctt tcatcagccc cgcctcgtcc tcgtggatcc ggcggcgctg accagcctgc
     3121 ccgagcgcga gttccgatcc gggctggctg aagtcgtcaa gcatggcatc gtgctggacg
     3181 ccgactactt cgccgacctg gagaagagcc tggtggccct gctcgggcgc gacatcgaga
     3241 cgctgacccg ggtcgtcgcg ggatcgtgcc ggctgaaggc gaacgtggtg gagcgagacg
     3301 agcaggaggc ggagctgcgc gcggtgctca actacgggca caccatcggc cacgcgatcg
     3361 aggcggtgac cgggtacgcg cgctggaccc acggcgaggc ggtgtccctg ggcatcgccg
     3421 ccgaggcccg actggccgag cgcctgggag tcgccagccg cgagaccgcc gcccggcaga
     3481 tcgatctgct ccgcgcggtg gggttgcccg tccgggactc ggcagcctcc ccgcgggcgg
     3541 tgctcgaggc tctcggccgc gacaagaagg cgcgcgaggg ccgcgtcccc ttcgtcctcg
     3601 ctccggccat cggccgattc accgtcgtct acgacgtgcc ccgcgaggcc gtgctcgaga
     3661 ccctccagga gctggggtga tccaacgggg gtccggggac ccccaccggg cgggtcccag
     3721 cttcgaggcc gacccgcggc tcatcgacct gggggtggcg cttttccttg tcctcgtcac
     3781 cgtcctcacc ttcctccccg ggctcgaggg cgccttcctg gactgggacg atcgcgagaa
     3841 cttcgtccac aacccgcact atcgcggcct gggccctgcc cagctccgct ggatgctcac
     3901 cacgggcctt ctcggccact ggatgccgct cacctggctc tcgcttgccg tggactggac
     3961 catctggggc atggatccct tcggctatca cctcaccagt ctcggcctcc acgcgatctg
     4021 cgcggtgctg gtctatgccc tggcccggcg cctcttctcc ctggccgccc cgagcgtccc
     4081 ccggacaacg catgtgctgg gcgccgcggt ggccgcgctc ctcttcgcca ttcatcccct
     4141 ccgcgtcgag tccgtggcgt ggatcaccga gcggagggat gtcctggccg gcgtattctt
     4201 cctgggggcg gtcatcgcct atctgggccg ctgggatccg gacggaaccg cgcgcccgcg
     4261 gccccggctc cggtacctgg ccgtgctggc cctctttgcg gccgcgctgc tctcgaaggc
     4321 catgtccgtg agcctgccgg tcgtcctcct gatcctcgat gtctacccgt tgtgccggct
     4381 cggcgggagc ccgcggggct ggctccgcaa ggacgcgcgc ccggtgtggc tggagaagat
     4441 cccgctcgtg gtgctgagcg tcggcgcgac g
//
LOCUS       DASUFA010000082         9719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1386357, whole genome shotgun sequence.
ACCESSION   DASUFA010000082 DASUFA010000000
VERSION     DASUFA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386357"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..977
                     /locus_tag="VFX87_03205"
     CDS             36..977
                     /locus_tag="VFX87_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX4993944.1"
                     /translation="MTTRIDRAAIERTYGLIAPYIRVTPVVETSGADFGLAAFPLAVK
                     LELFQHAGSFKTRGAFTNLLTRAIPAAGVVAASGGNHGAAVAYAAMRLGVRARVFVPT
                     VSSPAKVSRIRSYGAELVVIGDRYADALAASEVWGAESGALPVHAFDQEQTLLGQGTL
                     ALELARQAPALDTLLVPVGGGGLIGGIAVWCGGRPRVIGVEPEAAPTLTRALEAGKPV
                     DAPAGGLAADSLAPRRVGELMFPIAQAHIARVLLVSDAAIRRAQDALWATLRVVAEPG
                     GAAAFSALVSGAYAPGSGERVGVVVSGGNTTAVDFDR"
     gene            974..1246
                     /locus_tag="VFX87_03210"
     CDS             974..1246
                     /locus_tag="VFX87_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993945.1"
                     /translation="MSAKTPSRQESLTRALDFLAAGEWQRAHEIVQEDKSELAAWLHG
                     IVHTLEGDLDNARYWYRRTTREFPGPAAVQREIVAARLALAEEHRA"
     gene            complement(1217..2875)
                     /locus_tag="VFX87_03215"
     CDS             complement(1217..2875)
                     /locus_tag="VFX87_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4993946.1"
                     /translation="MLPSGDGNPFLRETVGGMLAAMAWSAGEREAIVAPDRRITYAQF
                     HREAQRVARGLVALGVKKNDKVALWLPTRPDWFFAQYGCAMIGAVVVALNTRYKAHEL
                     RYILRQSDATTLVCTDHAGPVDYLETLAEVLPALAGSVPGELLAEDFPRLRRVIVDAD
                     DPYPGCLRLRDVVEDGDAPEWAAALGSAAGPVGPDDAFTILYTSGTTSFPKGAVISHH
                     NCLPHGWWCGEVMRMTPADRVLHALPLSGTWGGLCIPLAAFSHGAAMILMETFEPAVA
                     LRLMEAERVTVWNGVDAMAIPMLEHPDLARRDRSRLRTGGFAATGGGVQGLFEAVVER
                     LGVRQAFQPYGMTEVNAMALVHDLDEPLESRARAGVWPADGIEARVVDPETGRDQPAG
                     HDGELWLRGRLVTKGYYEKPEETAKAFTPDGWFRTGDLAVRDGAGRVVFIGRLREVLR
                     ISHFMVAPGEIEDYLQTHPKVLQAFVVGVPDPRTNEAAVAYVIPRPGASVTEEEVIAH
                     CRGRIASFKVPRHVRVVADVPRTPGPHGDKVQKARLRAMFLGES"
     gene            2929..3522
                     /locus_tag="VFX87_03220"
     CDS             2929..3522
                     /locus_tag="VFX87_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993947.1"
                     /translation="MKCQDVRRTLSFVVDGQLALTEWAIIQGHLLECVECRTEFERLR
                     ALAGVRTRASQRRATAMTAAAMAVVLAVAAVGGVFIYQGRFPELPSWGTPPPPRAAAP
                     TVPAPEPVAPAAVPPPAVRPSPPPPARAKGTVEATPRGTAPPGSEPAARTPRPAAAAE
                     ALTEDRMPTTQARPPAVVNAPPGAEAMPTQGPSRGRR"
     gene            complement(3567..3731)
                     /locus_tag="VFX87_03225"
     CDS             complement(3567..3731)
                     /locus_tag="VFX87_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993948.1"
                     /translation="MRIEVEMVQEEGGIFRATAVSYPDVSVTGRTEKEALGLLLEAVG
                     KYLKTHSERT"
     gene            complement(3728..4528)
                     /locus_tag="VFX87_03230"
     CDS             complement(3728..4528)
                     /locus_tag="VFX87_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4993949.1"
                     /translation="MTIASPALPDPARSTPDVSEQRARIRAIIAPRTGERGLDIGCGF
                     GLLACELARDVGPIGRIMGVDAVPDMIIACEERARHDAVTGRTEFRQADPEAVPASSG
                     SFDFVTAMQVYEYMADVERGLAEAYRVLKPGGRLAVLDTDWESCVWHATDRERTARVL
                     RAWEQRFAHPHLPARLPELLRRGGFAVRTVTMIPVVNVEMSEQTYSPGMIDVVARFVA
                     SRGGITEDEAAHWAEDVRSQAARGEYFFSLSRYLFLAERARTSRGGAA"
     gene            complement(4656..5039)
                     /locus_tag="VFX87_03235"
     CDS             complement(4656..5039)
                     /locus_tag="VFX87_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4993950.1"
                     /translation="MARHRILVVDDEDNLRDVLVEVLTRDGHEVDSAVDGAEGLRRAQ
                     EHRYDLVVTDLRMPNLEGPDLYRAIRGRYPDDPPRFIFMSANTGIEEYAAFLSETGEP
                     ALEKPFNLADMRQVVLQVLAQKPRA"
     gene            complement(5041..6246)
                     /locus_tag="VFX87_03240"
     CDS             complement(5041..6246)
                     /locus_tag="VFX87_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX4993951.1"
                     /translation="MTTAPRSFVELLSTRARIGWGAPAAARPVTNALYEFGGGFPDPA
                     SFPYDGLIDATARMIRAEGAEALTYGEPQGYRGLRELVCHKYGLFEGLKVGLDNIIIA
                     NGSGHALSLAFSALVDPGDVILSEAPTFSGTLQTIRRHGPQVLDVPVDQDGLVVDVAR
                     EHLATLKKQGRRCKLIYTIVNFQNPSGPTLTLRRRRELIDLAHEYDTLILEDDAYGEL
                     RFEGEHLPSLYALDGGGRVMRAGTVSKILGAGFRLGWLCAPKEMIPAFQGFLFGGGVN
                     PFASRVATYFLRDNLAPHVALLVNVYRAKRDAMLRGLWEKLRGTDVEISRPEGGFFIW
                     IKLPTGTDTAKLYDAAVKARVQYTPGTSFFPNGGGERHIRLAFSFESPERCYEGAGLI
                     AQAILDARS"
     gene            complement(6257..6967)
                     /locus_tag="VFX87_03245"
     CDS             complement(6257..6967)
                     /locus_tag="VFX87_03245"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX4993952.1"
                     /translation="MRRLAWGAALALLAFLGPVASARADSVGELAERLRKATSVLGGM
                     ASGKEDAAVPREMLARARAIAVFPQVKKGAVIVGGRHGEGVMSVKRPSDGRWSAPAFF
                     TVGGGSFGLQLGGQVIDLVLVVMSDKGVESLLKSETTLGGEVSLTAGPKSLHDAANTD
                     GSFKAEIFSYARSTGFFAGASFEGATVQPDGKAIRTLYGAKADSRDVLLSGRYSVPPS
                     AQPFVKVLTTYSPPPKKR"
     gene            complement(7083..8273)
                     /locus_tag="VFX87_03250"
     CDS             complement(7083..8273)
                     /locus_tag="VFX87_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4993953.1"
                     /translation="MGVLDGIKVLEMARVPPAELPGMFLADMGADVLKIETPPDEAED
                     ATTKRRAAFAYVNRNKRSLALNMKAPEGQAIFKKLAAAADVIVEGFRPGVMKRLGGDY
                     ETLSATNPRLVYCSLSGFGHNGPYKDYPAHDGNYLSLAGVLNLIGEPDRKPIFPLNLV
                     ADYGGASMHGALGIMMALFARERTGRGQHVDVSYLDTSLALLAATPNMRFFWSDGIAP
                     KRGEGFLGGSYPYYAVYDTKDGKHLTIGCTEPWLWANFCKAINRPDMVKFARKPDQFV
                     RAANAEEVAAREEIEAIIRTRTRDEWYDFLVKADVCVGKVYEPDEVVADPQVQARDMI
                     VEVRHPVHGAVKQFGQPIKLSDTPGTIRSAAPHSGEHTDQVLRELGIPAGDIKTLRDK
                     KIVA"
     gene            complement(8284..9039)
                     /locus_tag="VFX87_03255"
     CDS             complement(8284..9039)
                     /locus_tag="VFX87_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX4993954.1"
                     /translation="MAVEALYALQNGFLGFERAGLFYGDRSAERVAIPVTCYLVRTGD
                     ATILFDTGISPRAVPGLVRQDPLCRFSDEDLLVHRLDSIGLEPKDVDLVVLSHLHFDH
                     AGGAALFDTSELVVQKEEYAYAQYPAAFFAPYYYRKNFDLPGYRWRLLDGDAELVPGV
                     TVLRSDGHTPGHQSLLVQLPRTGPVILAGDSCYWQRSIDEEIPPGVVWDPTRAMHSIK
                     RLKTLARLTGGRIFPSHDPAFWDTAVKAPDAYR"
     gene            complement(9042..>9719)
                     /locus_tag="VFX87_03260"
     CDS             complement(9042..>9719)
                     /locus_tag="VFX87_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX4993955.1"
                     /translation="DLAGTDKPVIGARLARHMHVSAPSITEALRRMQKDGYIRVGGHK
                     QIRLTTKGLGIAETMARRHRLIERWLTDVLGLDWSRAHDEAHRLEHALSPVVEERLAE
                     TLGMPSTCPHGNPIPGMPAPEAHSPIPLSQAPAGHPFVVERITEEAEADRQLLRFLWE
                     SGIRPGGRIVVSEIAPYAGTVSILLEGRTVTMGISAANKIWVYDPLVPADRPKPARAR
                     RPAKSRG"
BASE COUNT         1330 a         3402 c         3576 g         1411 t
ORIGIN      
        1 gcgaggcgcc cgtcaacctg gtaccatgac catccgtgac gacccggatc gaccgcgctg
       61 cgatcgagcg aacgtatggc ctgatcgcgc cgtacatccg cgtgacgccg gtggtggaga
      121 cgagcggggc tgatttcggc ctggccgctt tcccgctcgc cgtcaagctc gagctcttcc
      181 agcacgccgg ctccttcaag acccgcggcg cgttcaccaa cctgctcacc cgcgcgatcc
      241 cggccgccgg cgtggtcgcc gcgtccggcg gtaaccacgg cgccgcggtg gcgtacgcgg
      301 cgatgcggct gggcgtgagg gcgcgcgtct tcgtgcctac cgtatcctcg cccgccaagg
      361 tctcgcgcat ccggagctac ggcgccgagc tggtggtgat cggcgaccgc tacgccgacg
      421 cgctcgcggc gagcgaggtc tggggggccg agtcgggcgc gctgcccgtg cacgccttcg
      481 atcaggagca gaccctgctc ggtcagggca cgctcgcgct ggagctcgcg cggcaggccc
      541 cggccctcga cacgctgctg gtcccggtgg gcgggggcgg cctcatcggc ggcatcgcgg
      601 tgtggtgcgg cggccggccg cgggtgatcg gcgtcgagcc cgaggccgcg cccacgctca
      661 cccgggcgct cgaggcgggc aagccggtgg acgcccccgc gggcggcctc gcggcggatt
      721 cgctcgcccc gcgccgggtg ggggagctga tgttcccgat cgcgcaggct cacatcgccc
      781 gcgtcctgct ggtgagcgac gcggccatcc gccgggcgca ggacgcgctc tgggccacgc
      841 tgcgggtggt ggccgagccg ggcggcgccg ccgcgttctc ggcgctcgtg agtggcgcct
      901 acgcgcccgg gtcgggcgag cgcgtcgggg tggtggttag cggaggcaac accactgcgg
      961 tagacttcga tcgatgagcg ccaagacccc gtcgcggcag gagtcgctca ctcgcgccct
     1021 ggacttcctc gccgcggggg agtggcagcg cgcccacgag atcgtccagg aggacaaatc
     1081 ggagctggcc gcgtggctgc acggcatcgt gcacacgctg gagggcgatc tcgacaacgc
     1141 gcgctactgg taccggcgca ccacgcgcga gttcccgggc ccggccgcgg tgcagcggga
     1201 gatcgtcgcg gcgcgcctag ctctcgccga ggaacatcgc gcgtagccgc gccttctgca
     1261 ccttgtcgcc gtgcgggccc ggcgttcgag ggacgtcggc caccacgcgc acgtggcgcg
     1321 ggaccttgaa cgaggcgatg cgcccccggc agtgcgcgat gacttcctcc tcggtgacgc
     1381 tggcgccggg ccgcgggatc acgtaggcca ccgccgcctc gttggtgcgc gggtcgggca
     1441 cgccgacgac gaaggcctgc agcaccttcg gatgtgtctg caggtagtcc tcgatctcac
     1501 cgggcgccac catgaaatgg ctgatgcgca gcacctcgcg gagccgcccg atgaagacga
     1561 cgcgcccggc gccgtcgcgc accgccaggt cgccggtcct gaaccagccg tcgggcgtga
     1621 aggccttcgc ggtctcctcg ggcttctcgt agtagccctt cgtcaccagc cggccgcgca
     1681 gccacagctc gccgtcgtgg ccggcgggct ggtcccgccc ggtctccggg tccaccacgc
     1741 gggcctcgat accgtcggct ggccagacgc cggcgcgcgc gcggctctcg agcggctcgt
     1801 cgagatcgtg caccagcgcc atggcgttga cctccgtcat gccgtagggc tggaacgcct
     1861 ggcgcacgcc gagccgctcc accaccgcct cgaagaggcc ctgcacgccg ccgccggtgg
     1921 ccgcgaagcc gccggtgcgg aggcgcgagc gatcgcgccg cgccaggtcc gggtgctcga
     1981 gcatcgggat cgccatcgcg tccacgccgt tccacaccgt gacgcgctcg gcctccatga
     2041 ggcggagcgc caccgcgggc tcgaaggtct ccatcaggat catggcggcg ccgtggctga
     2101 acgcggccag cgggatgcag aggccgcccc aggtgcccga cagcggcagg gcgtggagga
     2161 cgcggtcggc cggggtcatg cgcatgacct cgccgcacca ccagccgtgc ggcaggcagt
     2221 tgtgatggct gatgaccgcg cccttgggga acgacgtcgt ccccgaggtg tagaggatcg
     2281 tgaacgcatc gtccggcccc accggccccg cggcgctgcc gagggcggcc gcccactcgg
     2341 gcgcgtcgcc gtcttcgacc acgtcacgga ggcgcaggca ccccggatag ggatcgtccg
     2401 cgtccacgat cacgcgacga agccggggga agtcttccgc caggagctcg ccgggcacgg
     2461 atccggcgag ggccgggagc acctcggcca gcgtctccag gtagtccacc gggcccgcgt
     2521 ggtcggtgca gacgagcgtg gtcgcgtccg actggcgcag gatgtagcgg agctcgtgcg
     2581 ccttgtagcg cgtgttgagg gccaccacca ccgcgccgat catcgcgcag ccgtactggg
     2641 cgaagaacca gtcgggtcgc gtgggcagcc agagcgccac cttgtcgttc ttcttgacgc
     2701 cgagggccac caggccgcgc gccacccgct gcgcctcgcg atggaactgc gcgtacgtga
     2761 tccgccggtc cggcgcgacg atggcctcgc gctcgcccgc gctccacgcc atcgcggcga
     2821 gcatgccgcc gacggtctcg cgcaggaacg gattgccgtc tccggatggc agcatgggcc
     2881 acatgctaac gctttacagc gggcgccaga tgtggaagat ggaaggtgat gaagtgccag
     2941 gacgtgcggc gtacgttgtc cttcgtcgtc gacgggcagc ttgcgctcac tgagtgggcc
     3001 atcattcagg ggcacctgct cgaatgcgtc gagtgccgga cggagttcga acggctgcgc
     3061 gccctggccg gcgtgcggac ccgagccagc cagcgccgcg cgaccgcgat gaccgcggcg
     3121 gcgatggccg tggtgctggc ggtggcggcc gtgggcgggg tcttcatcta ccaggggagg
     3181 ttccccgagc tgccgtcgtg ggggacgccg cccccgccgc gggccgctgc cccgaccgtg
     3241 ccggctcccg agccggtcgc accggccgcc gtcccgccgc cggccgtgcg accctcgccg
     3301 ccgccccccg cgcgagcgaa ggggaccgtc gaggcgacgc cgcggggtac ggcgcctccc
     3361 ggatcggagc cggccgcgcg cacgccgcgc ccggcggccg ccgccgaggc tctcaccgag
     3421 gaccgtatgc ctaccacgca ggcccgcccg cccgcggtgg tgaatgcccc gccgggcgcg
     3481 gaggcgatgc cgacccaggg tccgtcccgc ggccgccggt gacggccgcg caatcggtgg
     3541 ccggtctgag gcggaaggcc gcggagctag gtacgctcgg agtgggtctt caggtacttg
     3601 ccgacggctt ccagcaggag cccgagcgct tccttctcgg tgcgcccggt cacgctcaca
     3661 tccgggtagg acacggcggt ggcccggaag atcccgccct cttcctggac catctccacc
     3721 tcgatcctca tgccgcgcct ccccgcgagg tgcgcgcgcg ctcggcgagg aagaggtagc
     3781 gcgagaggct gaagaagtac tcgccccgcg cggcctggct ccgcacgtcc tcggcccagt
     3841 gggcggcctc gtcctcggtg atcccgcccc ggctggccac gaagcgcgcg accacgtcga
     3901 tcatccccgg gctgtaggtc tgctcgctca tctcgacgtt gacgaccggg atcatcgtca
     3961 cggtcctcac cgcgaacccg ccccgccgca gcagctcggg caggcgcgcg ggcaggtgag
     4021 gatgagcaaa ccgctgctcc caggcccgca ggacgcgggc ggtgcgctcg cggtcggtcg
     4081 cgtgccagac gcaggactcc cagtcggtgt cgagcacggc caggcgcccg cccggcttca
     4141 gcacgcggta cgcctcggcg aggccccgct cgacatcggc catgtactcg tagacctgca
     4201 tcgcggtgac gaagtcgaag ctgcccgacg aggcgggcac cgcctcggga tcggcctgcc
     4261 ggaactcggt gcggccggtc accgcgtcgt gccgggcccg ctcctcgcag gcgatgatca
     4321 tgtcgggcac cgcgtccacg cccatgatcc ggccgatggg gcccacgtcg cgggccagct
     4381 cgcaggcgag gaggccgaag ccgcagccga tgtcgaggcc gcgctcgccg gtgcggggcg
     4441 cgatgatggc gcggatgcgc gcgcgctgct ccgagacgtc gggggttgag cgcgcggggt
     4501 ccgggagggc ggggctggca atcgtcacgc cggaacgcta acagcgcccg gcgctcccgt
     4561 caaggagggg tcaggtctta catgccgaca tttctggcgt gccgcggtct agtgaccagg
     4621 ctgagaatgt cggaatgtaa gacctgaccc cagagctacg cgcgcggctt ctgggccagg
     4681 acctgcagca cgacctggcg catgtcggcc aggttgaacg gcttctccag cgcgggctcg
     4741 ccggtctcgg agaggaaggc ggcgtactcc tcgatgccgg tattggcgct catgaagatg
     4801 aagcggggcg ggtcgtcggg gtaccgcccg cggatcgcgc ggtagaggtc ggggccctcg
     4861 agattgggca tgcgcagatc cgtcacgacc agatcgtagc ggtgctcctg ggcccgccgg
     4921 aggccctcgg cgccgtccac cgcgctgtcc acctcgtggc cgtcgcgcgt cagcacttcc
     4981 accagcacgt cgcgcaggtt gtcctcgtcg tccacgacga ggatgcggtg ccgcgccatg
     5041 ctagctccgg gcgtcgagga tggcctgcgc gatcagcccc gcgccctcgt agcagcgctc
     5101 gggggactcg aagctgaagg ccagccggat gtggcgctcg ccgcccccgt tggggaagaa
     5161 ggacgtgccc ggggtgtact gcacgcgggc cttgaccgcg gcgtcgtaca gcttcgcggt
     5221 gtcggtgccg gtgggcagct tgatccagat gaagaacccc ccctcgggcc ggctgatctc
     5281 gacgtcggtg ccccgcagct tctcccagag cccgcgcagc atggcgtccc gcttggcgcg
     5341 gtagacgttg acgagcaggg ccacgtgggg ggccaggttg tcccgcagga agtaggtggc
     5401 cacgcgcgat gcgaaggggt tcacgccgcc cccgaagagg aagccctgga aggcggggat
     5461 catctccttg ggcgcgcaga gccagccgag gcggaagccc gcgccgagga tcttggacac
     5521 ggtgcccgcg cgcatcaccc gcccgccgcc gtccagcgca tacagcgagg gcaggtgctc
     5581 gccctcgaag cgaagctcgc cgtaggcgtc gtcctccagg atgagcgtgt cgtactcgtg
     5641 cgcgaggtcg atcagctccc gccgccggcg cagcgtcagc gtcggccccg acgggttctg
     5701 gaagttcacg atcgtgtaga tcagcttgca gcggcggccc tgcttcttga gcgtggccag
     5761 gtgctcccgc gcgacgtcca cgaccagccc gtcctgatcc accggcacgt cgagcacctg
     5821 cgggccgtga cggcggatgg tctggagcgt gccggagaac gtcggggcct cggacaggat
     5881 cacgtcgccc gggtccacga gcgcgctgaa ggccagcgag agcgcgtggc cggagccgtt
     5941 ggcgatgatg atgttgtcga ggccgacctt gagcccctcg aagaggccgt acttgtggca
     6001 caccagctcg cgcaggccgc ggtagccctg tggctcgccg taggtgagcg cctcggcgcc
     6061 ctcggcccgg atcatccggg cggtggcgtc gatgaggccg tcgtagggga aggacgcggg
     6121 gtccgggaac ccgcccccga actcgtacag cgcgttggtg acgggacgcg cggcggcggg
     6181 cgcgccccag ccgatgcggg cgcgcgtgga cagcagctcg acgaaggaac gaggggcggt
     6241 agtcatcggg tggctctcag cgcttcttgg gtggtgggga gtaggtcgtg agcaccttga
     6301 cgaagggctg cgcgctgggc ggcaccgagt agcggccgct cagcaggacg tcgcgggaat
     6361 cggccttggc gccgtagagc gtgcggatcg ccttgccgtc gggctgcacg gtggcgccct
     6421 cgaaggaggc gcccgcgaag aagccggtgg agcgcgcgta cgagaagatc tcggccttga
     6481 acgacccgtc ggtgttggcg gcgtcgtgca ggctcttggg gccggcggtc agagacacct
     6541 cgcccccgag cgtggtctcg ctcttgagca ggctctccac gcccttgtcg ctcatgacga
     6601 ccaggacgag gtcgatcacc tggccgccga gctgcaggcc gaagctgccg ccgcccaccg
     6661 tgaagaaggc gggcgcgctc cagcgtccgt cggacggccg cttgacgctc atcacgccct
     6721 caccgtgccg gccgcccacg atcaccgcgc ccttcttcac ctgggggaag accgcgatcg
     6781 cgcgcgcccg cgcgagcatc tcgcggggga ccgccgcgtc ctccttgccg gaggccatcc
     6841 cgccgagcac ggaggtcgcc ttccgcagcc gctccgcgag ctcgcccacg ctgtcggccc
     6901 gggcgctcgc gaccggcccg aggaatgcca gcagggcgag cgcggcgccc cacgcgagtc
     6961 gtctcatgac gaggatctta gcccatttcc gatcaggccg cccagtcggc gcacggcaac
     7021 cgcgaggggg aggtggagct gcgctccctc tgcgacgcgc agggtgaagg ccgcgatcgc
     7081 ggctaggcca cgatcttctt gtcgcgcagc gtcttgatgt cgcccgcagg gatccccagc
     7141 tcgcgcagca cctgatcggt gtgctcgccc gaatgcgggg cggcgctgcg gatcgtcccc
     7201 ggggtgtcgg agagcttgat cggctggccg aactgcttca ccgcgccgtg caccgggtgg
     7261 cgcacctcca cgatcatgtc gcgcgcctgc acctgcgggt cggccacgac ctcgtccggc
     7321 tcgtagacct tccccacgca gacgtcggcc ttcaccagga agtcgtacca ctcgtcccgg
     7381 gtgcgcgtcc tgatgatggc ctcgatctcc tcgcgcgcgg ccacctcttc cgcattggcc
     7441 gcgcgcacga actggtcggg cttgcgggcg aacttcacca tgtccggccg gttgatggcc
     7501 ttgcagaagt tcgcccagag ccagggctcg gtgcacccga tggtgaggtg cttgccgtcc
     7561 ttggtgtcgt agaccgcgta gtaggggtag gagccgccca ggaaaccctc gccgcgcttc
     7621 ggggcgatgc cgtcgctcca gaagaagcgc atgttgggcg tggccgcgag cagggccagc
     7681 gaggtgtcga ggtaggagac atccacgtgc tggccccgtc cggtgcgctc gcgggcgaac
     7741 agcgccatca tgatgccgag cgccccgtgc atgctggccc cgccgtagtc ggccaccagg
     7801 ttgaggggga agatcggctt gcggtccggc tccccgatga ggttcagcac gccggccagc
     7861 gacaggtagt tgccgtcgtg ggccgggtag tccttgtacg ggccgttgtg accgaagccg
     7921 gagagcgagc agtagaccag gcgcgggttc gtggccgaga gtgtctcgta gtcgccgccc
     7981 agccgcttca tcaccccggg ccggaagccc tccacgatga catcggcggc ggccgcgagc
     8041 ttcttgaaga tggcctggcc ctccggcgcc ttcatgttga gcgcgagcga gcgcttgttg
     8101 cgattgacgt aggcgaacgc ggcgcgccgc ttggtggtgg cgtcctcggc ctcgtcgggc
     8161 ggcgtctcga tcttgagcac gtccgcgccc atgtcggcga ggaacatgcc gggtagctcg
     8221 gcggggggaa cacgcgccat ctccagcacc ttgattccgt ccagcactcc catggggctc
     8281 tccttaccgg taggcgtcgg gcgccttgac ggccgtgtcc cagaacgcgg gatcgtggct
     8341 cgggaagatc cgcccgccgg tgagccgcgc gagcgtcttg agccgcttga tgctgtgcat
     8401 ggcccgggtc gggtcccaca cgacgccggg cgggatctcc tcgtcgatcg agcgctgcca
     8461 gtagcaggag tcgccggcca ggatcaccgg accggtccgg gggagctgca cgagcagcga
     8521 ctgatgcccc ggggtgtggc cgtcgctgcg cagcacggtc acgcccggga cgagctcggc
     8581 gtcgccgtcg agcagacgcc accggtagcc gggcaggtcg aagttcttgc ggtagtagta
     8641 gggggcgaag aacgcggccg ggtactgcgc gtacgcgtac tcctctttct gcaccaccag
     8701 ctccgacgtg tcgaagagcg cagccccgcc cgcgtggtcg aagtgcaggt ggctcaggac
     8761 gaccaggtcc acgtccttgg gctcgagccc gatcgagtcg agccggtgca ccaggagatc
     8821 ctcgtcgctg aagcggcaga gcggatcctg gcgcacgagg ccgggcaccg cgcgcgggga
     8881 gatgccggtg tcgaagagga tcgtggcatc gccggtgcgt accagatagc aggtgaccgg
     8941 gatggccacg cgctcggccg agcggtcacc gtagaagagt cccgcgcgct cgaaccccag
     9001 gaagccgttt tgcagcgcgt agagcgcttc gaccgccatg ctcagccgcg ggacttcgcg
     9061 gggcgccgcg ctcgggcggg cttcgggcga tcggccggca cgagcggatc gtagacccag
     9121 atcttgttgg cggcgctgat ccccatcgtg acggtgcggc cttccagcag aatggagacg
     9181 gtcccggcgt agggggcgat ctcgctcacc acgatgcggc cgcccggccg gatcccgctc
     9241 tcccagagga agcgcaggag ctggcggtcg gcctcggcct cctcggtgat gcgctcgacc
     9301 acgaacgggt ggccggcggg ggcctggctg agcgggatcg ggctgtgcgc ctccggggcc
     9361 ggcatgcccg ggatcgggtt gccgtgcggg caggtgctcg gcatgccgag ggtctcggcg
     9421 agccgctcct cgaccaccgg cgagagcgcg tgctcgaggc gatgcgcctc gtcgtgcgcg
     9481 cgcgaccagt cgagcccgag cacgtcggtc agccagcgct cgatgaggcg atgccggcgc
     9541 gccatggtct cggcgatgcc gagtcccttg gtggtcagcc ggatctgctt gtggccgccg
     9601 acgcggatgt agccgtcctt ctgcatgcgg cgcagcgcct cggtgatgga gggcgcggag
     9661 acgtgcatgt gccgcgcgag ccgcgcgccg atcaccggct tgtcggtgcc ggccagatc
//
LOCUS       DASUFA010000083         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_133439, whole genome shotgun sequence.
ACCESSION   DASUFA010000083 DASUFA010000000
VERSION     DASUFA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133439"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3306)
                     /locus_tag="VFX87_03265"
     CDS             complement(<1..3306)
                     /locus_tag="VFX87_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX4993956.1"
                     /translation="MSTMRRLALAVGLAAVACPGLGLAQEPVGVVTTLTGQVTRTASA
                     PGEAPAPLRFKDGVFGADTIRTAERSFVRMLLERKAVLTVRELSVLRITEDATQAAVD
                     LKAGGIAFSVARPRLRPGEMVLVRTPNAVAAVRGTTIVVETQGAVTHFHVLAGALDVA
                     SHANPTAWQRLQAPASLTVTGTVVGAPRRLDDATRDRLNADYLSLRVAELSAPAEVMA
                     QEQAKAVLLARLLSGDGSGGDEVELLAVADAQATQGAPGMQSPTQAPVTSPVVPATSA
                     AVPPPLHTWNNQTVTVTGDLLSVPGATTSTLGSSLARATGSTVTVTNEVVDVTGALTA
                     RAATPFVALQSSTLASRTLALVQTGSLTLTGGPLLEAVGGGASTTDDLLRVTGGGRLF
                     GPAGGPLLGLSGATLGVGNGTGDRLLELTGAGSRVSLGGALLDATGSTVSLTGTSLVE
                     VTTGAALDVAGASLVNLTGGALVLNGTATGFLFSSTTASTLGGGLLSTSGTDINAGIA
                     SPGNLLSVSGPLTSGSATPLLSLAGGDVRARNLGVVSSATGVLTLGGAFLDRAGAGNT
                     LVTTDDLLNVSAGGRLVAGGADALLRFTDTTVNAGNGSGDQLFQLTGAGSTATLAAGL
                     LEASNTAFTLTGSSLVDVSAGAVLTATGAGPLAALSGGALALGNATGFAISSTGASRI
                     AGSLLRTTNTGVTTTGDLVSVSGTLADTGTAPLLDLTGGAVAARNGILVSTANGRLSL
                     NGPALARTGGTLATTDDLFNISGSARLSSAGTGALLGFSGATVNVGNASGDQLFVVTG
                     AGSAATLAGSLLDASATTFTLTGSSFVEVSSGATLTAPGTAPLTALSGGALNLGGATG
                     FLVSSTSPSTIGGSLLRTTGSGVATTGNLVTVSGPLTDTGTAALLDLADGAVSARSGL
                     AISSASGQLSLNGPALSRTGGTLTLTDDLVSITAGGRLTDTGAGSLLAFSTGTVNIGN
                     AAGDQLFVVNGAGSQATLAGPVLGTAGTALSLTGTALVEVSTGATLTDTATARLVSLS
                     GGALTLGSATNAFLVNTSGTASLAGGLLDATGTDITAAGDFVRALGGGRIVVIGSSAP
                     LLSLTGGTHQFGAT"
     gene            3587..3910
                     /locus_tag="VFX87_03270"
     CDS             3587..3910
                     /locus_tag="VFX87_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993957.1"
                     /translation="MAAKGIRAVITGVGLALVSTGCASHGPYLSTGQTQLGTSPHQIG
                     PVTVVIRPQSEVEMICRLRSPLSAPTGRIHGCYVPADRTIISTADPYVLMHEFKHYFE
                     GAWHQ"
     gene            4010..4618
                     /locus_tag="VFX87_03275"
     CDS             4010..4618
                     /locus_tag="VFX87_03275"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX4993958.1"
                     /translation="MSPKLGMAPVRREQIVRATIRCLARDGYAGLTMKRVAAEAAVSQ
                     GILHYYFRDKAAMLAAAARRVTVDLDRRVATESRGARTARERLRAVIRACLRTAVDNR
                     DFWTVFIEFWGEAFHERRLAAVNRQAYARARRLIGAGLARGVATGEFRRIDVKEAAAL
                     VLAVLDGLSLQLTFDRGLMTVPRAARAAETVLLRYLVKGDPT"
BASE COUNT          657 a         1658 c         1769 g          625 t
ORIGIN      
        1 ggtcgcgccg aactggtgcg tgccgccggt gagcgagagc agcggcgcgc tcgagccgat
       61 caccacgatg cgcccgccgc ccagcgcccg cacgaagtcg cccgccgcgg tgatgtcggt
      121 gccggtggcg tcgaggaggc cgccggccag gctcgcggtg cccgaggtgt tcaccaggaa
      181 ggcgttggtc gccgagccga gcgtgagcgc gccgcccgag aggctgacga gccgcgcggt
      241 cgcggtgtcc gtcagcgtcg cgccggtcga gacctcgacc agggcggtgc ccgtgagcga
      301 gagcgcggtg cccgcggtgc ccagcaccgg gccggccagg gtggcctggg atcccgcgcc
      361 gttcaccacg aagagctggt cgccggccgc gttgccgatg ttgacggtgc ccgtggagaa
      421 ggcgagcagc gagcccgcgc ccgtgtcggt gagccggccg cccgccgtga tgctgaccag
      481 atcgtcggtc agggtcaggg tgcccccggt gcggctgagg gcgggcccgt tcaggctgag
      541 ctggccgctg gccgacgaga tcgcgagccc actacgtgcc gagaccgcgc cgtcggcgag
      601 gtcgagcagc gcggcggtgc cggtgtcggt gagcggtccg gacacggtca cgaggttccc
      661 ggtggtggcg acgccggaac cggtggtgcg gagcaggctc ccgccgatcg tgctggggct
      721 ggtcgagctc accaggaagc cggtggctcc gcccaggttc agcgcgccac ccgagagggc
      781 ggtgagcggc gccgtgccgg gcgcggtgag cgtcgcgccg ctcgatacct cgacgaagga
      841 cgagccggtg agggtgaacg tggtggcgga ggcatcgagt agagagccgg caagcgtcgc
      901 cgccgatccg gctcccgtga ccacgaagag ctggtcgccg gaggcgttgc ccacgttgac
      961 ggtggcgccc gagaagccca ggagcgcgcc ggtcccggcg ctcgagagcc gcgcgctccc
     1021 ggagatgttg aagagatcgt cggtggtggc cagcgtaccg cccgtgcggg ccagcgcggg
     1081 cccgttcagg ctgaggcgac cgttcgcggt ggagaccaga atgccgttgc gggccgccac
     1141 cgcgccgccg gtgagatcga gcagcggggc ggtgccggtg tcggcgagcg tcccggagac
     1201 gctgacgagg tcgccggtgg tggtcacgcc ggtattcgtg gtgcgcagca ggctgcccgc
     1261 gatccggctc gctccggtcg agctgatggc gaagccggtc gcgttgccga gggcgagcgc
     1321 gccgcccgag agggcggcga gagggccggc gccggtcgcg gtgagtaccg cgccggcgga
     1381 cacgtcgacg agggacgagc cggtgagggt gaacgcggta ttggaggcct cgagcaggcc
     1441 ggcggccagc gtcgcggtcg agccggcgcc ggtgagctgg aagagctggt cgccgctgcc
     1501 gttgccggcg ttgacggtcg tgtccgtgaa gcgcagcagc gcgtcggcgc cgcccgccac
     1561 gagccgcccg cccgccgaga cgttcaggag gtcgtcggtc gtgaccagcg tattccccgc
     1621 gcccgctcgg tccaggaagg cgccgccgag cgtgagcacg ccggtcgccg acgacaccac
     1681 gcccaggttc cgcgcgcgca cgtcaccgcc ggccagcgag aggagcgggg tggccgagcc
     1741 gctggtgagc ggacccgaca cgctcaggag attgccgggt gaggcgatgc ccgcgttgat
     1801 gtcggtgccg ctcgtgctga gcaggccgcc gcccagcgtg gacgcggtcg tcgagctgaa
     1861 caggaagccg gtggccgttc cattcagaac cagcgcgccg ccggtcaggt ttaccaggct
     1921 ggcgccggcc acatccaggg ccgcacccgt cgtcacctcg accagggagg tgccggtgag
     1981 gctcacggtg gagcccgtcg cgtcgaggag cgcgcccccg aggctcactc gcgagcccgc
     2041 tcccgtcagc tccagcaggc ggtcaccggt cccgttgccg acaccgagcg tggctccgga
     2101 gaggccgaga agcggcccgc ccgcgggtcc gaagaggcgg ccgccccccg tgacccggag
     2161 gagatcgtcg gtggtgctgg caccgccgcc caccgcctcg aggagcgggc caccggtcag
     2221 ggtcaggctg cccgtctgga ccagggccag cgtgcgggac gccagcgtgc tggactggag
     2281 cgcgacgaac ggggtcgccg cgcgggcggt cagggcgccg gtgacgtcca cgacttcgtt
     2341 ggtgaccgtg accgtgctgc cggttgccct cgcgaggctc gagccgagcg tgctggtggt
     2401 cgcgcccggc acgctgagga gatcgcccgt gacggtgacg gtctgattgt tccaggtgtg
     2461 gagcggcgga ggcaccgccg cggaagtggc cggcacgacc ggtgacgtca ccggcgcctg
     2521 cgtcgggctc tgcatgccgg gcgcgccctg ggtcgcctgc gcgtcggcca ccgcgagcag
     2581 ctcgacctca tcacctccgg acccgtcgcc gctgagcagc cgcgccagca gcaccgcctt
     2641 ggcctgctcc tgggccatca cctccgcggg cgcgctcagc tcggccacgc gcaggctcag
     2701 gtagtcggcg ttgagccggt cgcgggtagc gtcgtcgagc cggcgcggcg cgccgaccac
     2761 cgtcccggtc acggtcaggc tggcgggcgc ctgcagccgc tgccacgcgg tcgggttggc
     2821 gtgggaagcc acgtcgaggg ccccggccag aacgtggaag tgcgtgaccg cgccctgcgt
     2881 ctcgaccacg atcgtggtgc cgcgcaccgc ggccaccgcg ttcggcgtgc ggaccaggac
     2941 catctcgccc gggcgcaggc ggggtcgggc aacgctgaac gcgatgccgc ccgccttcag
     3001 atccaccgcc gcctgggtcg cgtcttccgt gatgcggagg accgacagct cacggacggt
     3061 cagcaccgcc ttgcgctcga gcagcatgcg cacgaaggag cgctcggccg tccggatcgt
     3121 gtccgcgccg aagacgccgt ccttgaaccg caacggtgca ggcgcctcgc ccggggcgct
     3181 cgccgtccgg gtcacctgcc cggtcagggt ggtgaccacg ccgaccggct cctgggccag
     3241 accgaggccc gggcaggcca cggcagccag gccgactgcc agcgccaacc gccgcatggt
     3301 gctcatgcac cattctcaga gcaacgtcca tgccgacctt caacatattg aaattgctgg
     3361 gatcggaggc atgtatggcc gggcgaacgc tcgctacctg aggggggacc cacaaatttc
     3421 gatggaagac cggctatggt cgagtcgcca tagtcctcga cgggatacca gaggggcgag
     3481 accacccccc tcgtaccgcc gtcttgtacg cgcctggaag atatggcagc cggtgtggct
     3541 cgatcccggg gtggcgctgg cacctgggtt gccttgctga tggtgtatgg cagcgaaggg
     3601 gatccgggcg gtgatcaccg gcgtcgggct ggcactggtc tcgacggggt gcgcgagcca
     3661 cggcccgtac ctgagcacgg gacagaccca gctcggcact tctccgcacc agatcggccc
     3721 ggtgacggtg gtgatccgtc cgcagtccga ggtcgagatg atctgccggc tccggagccc
     3781 gctatcggcc cccaccggcc ggatccacgg ctgctacgtg cccgccgacc ggaccatcat
     3841 ctcgaccgcg gacccgtacg tgctgatgca cgagttcaag cattacttcg aaggcgcctg
     3901 gcaccagtag tccccggttc ccgcccggcc gcttgacgcg gccgccttcc cgctgcatac
     3961 tccgacccgg ataattggtc gaccgaccaa taaaaaacgg aggcgcggcg tgtcccccaa
     4021 gctcggcatg gccccggtgc ggcgcgagca gatcgtccgc gccaccatcc ggtgccttgc
     4081 ccgcgatggc tacgccggtc tcaccatgaa gcgcgtggcc gccgaggccg cggtgagcca
     4141 gggcatcctc cactactact tccgcgacaa ggccgcgatg ctggccgcgg cggcccggcg
     4201 cgtgaccgtg gacctcgacc gtcgcgtggc caccgagtcg cgcggggccc ggaccgcgcg
     4261 ggagcggctg cgcgccgtca tccgcgcgtg cctgcgcacc gcggtggaca atcgcgattt
     4321 ctggaccgtg ttcatcgagt tctggggcga ggccttccac gagcggcggc tggccgcggt
     4381 gaaccggcag gcgtacgcgc gcgcccggcg cctcatcggc gcgggcctgg cccgcggcgt
     4441 cgcgaccggc gagttccgcc ggatcgacgt gaaggaagcc gccgcgctgg tgctggccgt
     4501 cctcgacggc ctctcgctcc agctcacctt cgaccgcggc ttgatgacgg tgccgcgcgc
     4561 ggcccgtgcc gcggagaccg tgctcctccg atacctcgtg aaaggagacc cgacatgacc
     4621 acgccgccag aacacgcgaa agccaagacc tacctgctcg agaagggcac ccaggccccc
     4681 gtcgcccaga tccgcgagcg cgtcgccga
//
LOCUS       DASUFA010000084         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1155071, whole genome shotgun sequence.
ACCESSION   DASUFA010000084 DASUFA010000000
VERSION     DASUFA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155071"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..907
                     /locus_tag="VFX87_03280"
     CDS             101..907
                     /locus_tag="VFX87_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4993959.1"
                     /translation="MTDKVQVEIKSPVATITLNKPDRLNALDVEMWEGISAAAALIDA
                     APEVRVAVLTGAGGAFCAGLDLKAGSTLSRPAGETPPQTLVGIRTHLKHLQECFTRLE
                     SCRVPVIAGIQRACLGGGLELALCCDVRLAAEGTIFSIPEVQIGIVPDMGGTQRLPRT
                     VGIGKAKELIYSARRIDAAEALRIGLVNGVYPAADLPARVAELAAEIAANAPLAVQAA
                     KQSIDGTYWRERESWLAWEADHAAPVLLSEDRLEGMRAGAERKRANFKGR"
     gene            916..1944
                     /locus_tag="VFX87_03285"
     CDS             916..1944
                     /locus_tag="VFX87_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4993960.1"
                     /translation="MKALAFHEHGGLDKLKYQDVADPKIGAGDVLVRVKAAALNHLDL
                     FVREGMPGLKLPLPFWSGCDIAGEIAEVGGGVPGLAAGDRVVVNPNLNCGRCEYCQKG
                     QHSLCVTYGILGEHAHGGLAEYVKVDGRKVLKLPDAVPYEDAAAFVLVNMTAWRMLVS
                     QARLRPGEDLLIIGVGGGVSSTGVQIGKLCGARVWVTSSSDEKLERARALGADECINY
                     AKEDWVKVIAQKTGRRGVDVVLENVGAATWPGAIRVLAKGGRLVTCGGTSGPICETDV
                     RQVFWKQISIIGSTMSTNAEFAEVMGQLFRGRLKAIVDSVTPLENGVAAQQRLAEAKQ
                     FGKIVLIP"
BASE COUNT          311 a          698 c          727 g          281 t
ORIGIN      
        1 cggggctgct cgaccgcacc gggctgtatc agccctaccc gccgcgccag gacgatccgc
       61 gcctgcccgc gctggtcaag gccttcaacg ggagcccaac atgacggaca aggtccaggt
      121 cgagatcaag agcccggtcg ccaccatcac gctcaacaag cccgatcggc tcaatgcgct
      181 ggacgtggag atgtgggaag ggatcagcgc cgccgccgcc ctcatcgacg cggcgccgga
      241 ggtgcgcgtc gccgtcctca ccggcgcggg cggcgccttc tgcgcgggcc tcgacctcaa
      301 ggccggctcc acgctgtccc ggcccgcggg cgagaccccg ccccagaccc tcgtggggat
      361 ccgcacccac ctcaagcatc tccaggagtg cttcacccgg ctggaatcct gccgcgtgcc
      421 cgtgatcgcg ggcatccagc gcgcctgtct gggcggcggc ctcgagctgg cgctgtgctg
      481 cgacgtccgg ctcgccgccg agggaacgat cttctccatt cccgaggtgc agatcggcat
      541 tgtgcccgac atgggcggca cccagcgcct gccgcgcacg gtgggaatcg gcaaggccaa
      601 ggagctgatc tactccgcgc ggcgcatcga cgccgccgag gcgctccgca tcgggctcgt
      661 caacggggtg taccccgccg ccgacttgcc cgcgcgggtg gcggagctcg ccgccgagat
      721 cgccgccaat gcgccgctcg ccgtgcaggc ggcgaagcag agcatcgacg gcacctactg
      781 gcgcgagcgg gagtcgtggc tcgcctggga ggccgatcac gccgcgcccg tgctcctctc
      841 cgaggaccgg ctcgagggca tgcgggccgg cgcggagcgc aagcgcgcca acttcaaggg
      901 gagataggca ccggcatgaa ggcgctcgcg ttccacgagc acggcgggct cgacaagctc
      961 aagtaccagg acgtggccga ccccaagatc ggcgccggcg acgtgctcgt ccgcgtcaag
     1021 gcggcggcgc tcaatcacct ggacctcttc gtccgtgagg ggatgcccgg gctcaagctg
     1081 cccctgccgt tctggagcgg ctgcgatatc gccggggaga tcgccgaggt gggcggcggc
     1141 gttcccggcc tggcggcggg cgaccgtgtg gtcgtcaacc cgaacctgaa ttgcgggcgc
     1201 tgcgagtact gccagaaggg ccagcacagc ctctgcgtga cctacggcat cctgggcgag
     1261 cacgcgcacg gcgggctggc cgagtacgtg aaggtggacg gccggaaggt gctcaagctc
     1321 cccgacgcgg tgccctacga ggacgccgcc gccttcgtcc tcgtcaacat gaccgcctgg
     1381 cgcatgctgg tgtcccaggc gcggctgcgc ccgggggagg acctcctcat catcggcgtg
     1441 ggcgggggcg tgtcgtccac cggggtgcag atcggtaagc tgtgcggcgc gcgggtctgg
     1501 gtgacctcct ccagcgacga gaagctcgag cgggcgcggg cgctcggcgc ggatgagtgc
     1561 atcaactacg cgaaggaaga ctgggtgaag gtgatcgccc agaagacggg gcggcggggc
     1621 gtggacgtgg tgctggagaa cgtgggcgcc gctacctggc cgggggccat ccgggtgctg
     1681 gccaagggcg gccggctcgt gacctgcggc ggcacctccg ggcccatctg cgagacggac
     1741 gtgcggcaag tgttctggaa gcagatctcc atcatcggct ccaccatgtc caccaatgcc
     1801 gagttcgccg aggtcatggg ccagctcttc cgcggccggc tcaaggccat cgtggattcc
     1861 gtgacgccgc tcgagaacgg cgtggccgcg cagcagcggc tcgccgaggc caagcagttc
     1921 ggcaagatcg tactgattcc gtagcccgaa tgaccgacga gacggagcgc tactacgccg
     1981 agcgggcggc gcagtacgat cgcgtctatg cgcatcc
//
LOCUS       DASUFA010000085         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_353251, whole genome shotgun sequence.
ACCESSION   DASUFA010000085 DASUFA010000000
VERSION     DASUFA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353251"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFX87_03290"
     CDS             <1..305
                     /locus_tag="VFX87_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993961.1"
                     /translation="GGRRGGVAHLEWRVAVPAPEIRLGGVVGTGGVTLAPFASAGWAD
                     GALPGVSWAPTGGVRPVAGLALEWPMGLLRVEAGVALRTGQAGVTVDVSRAWWGIL"
     gene            356..1576
                     /locus_tag="VFX87_03295"
     CDS             356..1576
                     /locus_tag="VFX87_03295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4993962.1"
                     /translation="MTVATATATGQFEVYTAGVAAPPIWARPDPALATAGIEGELVVA
                     RVRVLAMGLLLIAPTWNVTHYPNDPVHVTGFTVTLGAALVSVGILRALRRGRWRPWIG
                     FASSTFDVSMVSLALVSFLIVGSPMIALNSKVTFEMYFLALVATCLRYDARICIAVGL
                     LALAQYGGLWAFAAANYDLSDPAYVAAAGPYSRVDLATRLILLGVATILAVTIVRRAQ
                     RLLYLASRDRLTGLFNRGHFDRALRAAMERAKRAGQPLSVALVDVDHFKSINDSYGHA
                     LGDRALVQVAQLLDRTMRRTDVVARYGGEEFGILLGGTGRDAARQRLEAIRREVADTP
                     IDLGGGQVLHINFSAGIAGIPDDAGVTTAKGLVAKADERLLAAKRLGRGRCVGTEVVP
                     EAYPAPPAGSILRD"
     gene            1617..>3418
                     /locus_tag="VFX87_03300"
     CDS             1617..>3418
                     /locus_tag="VFX87_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX4993963.1"
                     /translation="MPFTDEWLLPTVEPLVAPEMMAGLQREQATTNLSLWEALVQRRA
                     LTDQQILQAVAQRFRLPVADLSSADLEARLAVPEGLARKHHVLPLRISDAVLEVATSN
                     PFDINAEKELAFATGREVRMALAPPVRLREKIDEHYRPEEVLSKLLGGMDEPMVRQLT
                     DEPDDVNVSADEASARPIIRLVDMLLADGVTSRASDIHIEPSEGGVVVRYRIDGVLRQ
                     VMRIPRNAGLPVISRVKIMSGLDIADRLRPQDGRARVQVNQEKIDLRVSTLPASLGEK
                     VVIRILNSKATVLALEALGLHDDERRQLIKLLDHKEGIVLVTGPTGSGKTTTLYSAIR
                     LIQGEGVNIVTVEDPVEYKLGQNIVQVQVNEKAGLTFASALRSILRQDPDVVLVGEIR
                     DRETAQIAVQASLTGHLVLSTLHTNDAPNAVTRLVDMGIEAYKLGSALRGIVAQRLMR
                     RLCPVCREALPVPPGDLLPEQARGRVPEDAQLYKAVGCAECAQTGYRGRFSITEILTM
                     TQDLEQLIGEGGTADRIGRMARQQGMKSLWESGLRHVTAGDTSIDELLRVCDVPGHGG
                     LDADAPTRGRADAPAAAPVRGRAAAPAAPPAAEPP"
BASE COUNT          454 a         1203 c         1293 g          468 t
ORIGIN      
        1 tcggcggccg gcgcggcggg gtggcgcacc tcgagtggcg ggtggccgtg ccggcgcccg
       61 agatccggct cggcggtgtc gtggggacgg gcggcgtcac cctggcgccg ttcgcgtccg
      121 cgggatgggc cgacggcgcc ctgccgggtg tatcgtgggc cccgaccggc ggcgtgcgtc
      181 cggtggcggg cctcgcgctg gagtggccga tgggcctcct ccgggtcgag gcgggcgtgg
      241 cgctccgcac tgggcaggcg ggcgtcacgg tggacgtgag ccgggcgtgg tgggggatcc
      301 tctgactggc cgctgatccc tactttatgc aggtctctcc gagcgggggt gccggatgac
      361 ggtggcgacg gcgacggcga cgggccagtt cgaggtctac accgcgggcg ttgccgcgcc
      421 gcccatctgg gcccggcccg accccgcgct ggccaccgcc ggcatcgagg gcgagctggt
      481 cgtggctcgg gtccgggtgc tcgcgatggg tctcctgctc atcgccccca cctggaatgt
      541 cacccattac ccgaacgacc cggtgcacgt cacgggcttc accgtgacgc tgggcgccgc
      601 gctcgtctcg gtggggatcc tccgggccct ccggcggggg cggtggcggc cctggatcgg
      661 cttcgcgtcg agcacgttcg atgtctcgat ggtgagcctg gccctggtga gcttcctgat
      721 cgtcggctcc ccgatgatcg cgctcaactc gaaggtcacc ttcgagatgt acttcctggc
      781 gctggtggcc acgtgcctcc gctacgacgc gcgcatctgc atcgccgtgg ggctgctggc
      841 gctggcccag tacggggggc tctgggcgtt cgcggcggcg aactacgacc tgtccgaccc
      901 ggcatacgtc gcggcggcgg ggccgtactc ccgggtggat ctcgccaccc gcctgatcct
      961 gctcggcgtc gccacgatcc tcgccgtgac catcgtgcgg cgcgcgcagc ggctcctcta
     1021 cctcgcctcg cgcgaccggc tcaccgggct cttcaaccgg gggcacttcg accgggcgct
     1081 ccgcgccgcg atggagcgcg cgaagcgcgc gggccagccg ctctcggtgg cgctggtgga
     1141 cgtggaccac ttcaagtcca tcaacgacag ctacggccat gccctgggcg accgcgcgct
     1201 ggtgcaggtg gcccagctgc tcgaccgcac gatgcggcgc accgacgtcg tcgcccggta
     1261 cggcggcgag gagttcggca tcctgctggg cgggacgggg agggatgccg cccggcagcg
     1321 cctggaggcg atccgccgcg aggtggcgga caccccgatc gacctcggcg ggggccaggt
     1381 gctccacatc aacttcagcg ccgggatcgc gggcatcccg gacgacgcgg gggtgacgac
     1441 ggcgaagggg ctggtggcga aggccgacga gcggttgctc gcggccaagc ggctggggcg
     1501 ggggcgctgc gtcggcaccg aggtcgtccc ggaggcctac ccggccccgc cggcggggtc
     1561 tatcttgcga gactgacgca agttagcgga gcgctccccc gcctcccgga accgcgatgc
     1621 ccttcaccga cgagtggctc ctcccgaccg tcgagcccct ggtggccccg gagatgatgg
     1681 ccgggctcca gcgggagcag gcgaccacca acctctccct ctgggaggcg ctggtgcagc
     1741 gccgcgcgct caccgaccag cagatcctgc aggcggtggc ccagcgcttc cgcctgccgg
     1801 tggccgacct ctccagcgcc gacctcgagg cgcggctggc ggtgccggag gggctcgccc
     1861 ggaagcacca cgtgctcccg ctcaggatct ccgatgccgt gctcgaggtg gccacctcga
     1921 accccttcga catcaacgcc gagaaggagc tggcgttcgc caccggccgc gaggtgcgga
     1981 tggcgctcgc gccgccggtg cggctccggg agaagatcga cgagcattac cgccccgagg
     2041 aggtgctctc gaagctgctc ggcgggatgg acgagccgat ggtccggcag ctcaccgacg
     2101 agccggacga cgtcaacgtc tccgccgacg aggccagcgc ccggccgatc atccggctgg
     2161 tggacatgct gctcgccgac ggcgtcacca gccgggccag cgacatccac atcgagccga
     2221 gcgagggcgg ggtggtggtc cgctaccgga tcgacggcgt gctgcggcag gtgatgcgga
     2281 tcccgcggaa cgccgggctc ccggtgatct cgcgggtgaa gatcatgagc gggctcgaca
     2341 tcgcggaccg gctgcgaccc caggacgggc gcgcccgggt gcaggtgaac caggagaaga
     2401 tcgacctccg ggtctcgacc ctgccggcgt cgctgggcga gaaggtggtg atccggatcc
     2461 tcaactcgaa ggccacggtg ctggccctcg aggcgctcgg cctccacgac gacgagcggc
     2521 gccagctcat caagctgctg gaccacaagg agggcatcgt cctggtgacc gggccgaccg
     2581 gctcgggcaa gacgacgacg ctctactcgg cgatccggct gatccagggc gagggcgtca
     2641 acatcgtcac ggtggaggac ccggtcgagt acaagctggg ccagaacatc gtgcaggtgc
     2701 aggtgaacga gaaggcggga ctgacgttcg cctcggcgct ccgctccatc ctgcggcagg
     2761 acccggacgt ggtgctggtg ggcgagatcc gcgaccggga gaccgcccag atcgcggtgc
     2821 aggcgtcgct caccggccac ctggtgctct cgaccctgca caccaacgac gcgcccaacg
     2881 cggtgacgcg cctggtggac atgggcatcg aggcgtacaa gctcggctcg gcgctccgcg
     2941 gcatcgtcgc ccagcggctg atgcgccggc tctgcccggt ctgccgggag gcgctgccgg
     3001 tgccacccgg cgacctcctg ccggagcagg cacgggggcg ggtgccggag gacgcgcagc
     3061 tctacaaggc cgtcggctgc gcggagtgcg cccagaccgg ctaccgcggg cgcttcagca
     3121 tcaccgagat cctgacgatg acgcaggacc tcgagcagct gatcggcgag gggggcaccg
     3181 ccgaccggat cgggcggatg gcgcgccagc aggggatgaa gtcgctctgg gagagcggcc
     3241 tccgccacgt gacggcgggc gacacctcca tcgacgagct gctgcgggtg tgcgacgtgc
     3301 cggggcatgg ggggctggat gcggacgccc cgacgcgcgg acgcgccgac gcgccggccg
     3361 ctgccccggt gcgcggacgt gccgccgcgc ccgccgcgcc accagccgcc gagccgcc
//
LOCUS       DASUFA010000086         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1155396, whole genome shotgun sequence.
ACCESSION   DASUFA010000086 DASUFA010000000
VERSION     DASUFA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155396"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..809
                     /locus_tag="VFX87_03305"
     CDS             102..809
                     /locus_tag="VFX87_03305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX4993964.1"
                     /translation="MLHGVEKARFVSAMFSRIARRYDLMNGLMTLGMHHSWRRVAARQ
                     TIASPDGPALDLATGTGDLAIDLAEVHPHRTVIAADFSLGMLAVARDKLAGLEETRRV
                     RLLAADALALPFGTRTFACVTSAFLLRNLADLEQGLREMQRVTRPGGRVVALEITQMR
                     APGFTALFRFYFHHVVPRIGQLVGGDREAYSYLPQSVDRFVTPGELVRLMEKVGLRGV
                     TYRRLGLGTVTVHTGIA"
     gene            complement(819..1094)
                     /locus_tag="VFX87_03310"
     CDS             complement(819..1094)
                     /locus_tag="VFX87_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993965.1"
                     /translation="MAYSLTDSLKHNRFSIVSVDKAAGTVRVRGEAEVCTDLSCGETL
                     VVTDEGTTRDLEQLYAGDIVTLEQKDGRAQQIRVVRRVWEEYSSPEW"
     gene            1200..1871
                     /locus_tag="VFX87_03315"
     CDS             1200..1871
                     /locus_tag="VFX87_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX4993966.1"
                     /translation="MTTPRGRIAETFYSIQGEGATAGLPAVFVRLQGCSVGCGWCDTK
                     YSWDPAAGREVDLPALLDEVAAFPCRRVVITGGEPLESPLFTPLAAALAARGYVVEVE
                     TSGTRAPAPEADAGIQWNVSLKLSGSGVPEATRVNPAAIRALLARGAWWKFVVSEPGE
                     IGEALRLAERFALPRERVLLQPEGLRAEDLASRTPWLVEACKAHGFRFSPRLHILIWG
                     PKRGV"
     gene            1871..2563
                     /gene="queC"
                     /locus_tag="VFX87_03320"
     CDS             1871..2563
                     /gene="queC"
                     /locus_tag="VFX87_03320"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX4993967.1"
                     /translation="MPARRAVVLLSGGLDSTTAFAVARAEGFDCYALSFDYGQRHDRE
                     LESARRVAAALGAKEHLVLRLDLRAIGGSALTADIPVPKGRSHEAMGTGIPVTYVPAR
                     NTIFLSHALAWAEVLESQDIFLGVNALDYSGYPDCRPEYIEAFERMANLATKAGVEGR
                     SRLTIHTPLIRMTKAQIVKRGAELGVDFGLTWSCYEPQPDGRACGLCDSCLLRHKGFA
                     EAGLRDPVPAAH"
BASE COUNT          355 a         1031 c          969 g          447 t
ORIGIN      
        1 agcgatctat cgcggctgga cgtcgccttc ttcaacgtca acggctacat cgcggtgatc
       61 ctcttcttct cggtgctggg aggccggctc gcgtgaccga gatgctccac ggcgtcgaga
      121 aggcccgctt cgtcagcgcg atgttctcgc gcatcgcccg ccgctacgac ctgatgaacg
      181 gtctcatgac gctcggcatg caccacagct ggcgccgggt ggccgcgcgg cagaccatcg
      241 cctcgcccga cggccccgcg ctcgacctgg ccaccggcac cggcgatctc gccatcgatc
      301 tggccgaggt ccacccgcac cgcacggtga tcgcggccga tttctcgctc ggcatgctgg
      361 cggtggcgcg agacaagctg gccgggctcg aggagacgcg gcgcgtgcgc ctcctggccg
      421 ccgacgcgct ggccctgccc ttcgggacgc gcaccttcgc gtgcgtgacg agcgcgttcc
      481 tgctgcgcaa cctggccgac ctcgagcagg gcctgcgcga gatgcagcgg gtgacgcgcc
      541 cgggcggccg cgtggtcgcg ctcgagatca cccagatgcg cgcacccggc ttcaccgcgc
      601 tcttccggtt ctacttccac cacgtggtgc cgcggatcgg ccagctcgtg ggcggcgacc
      661 gcgaggcgta cagctacctg ccgcagtcgg tggaccgctt cgtgacgccg ggcgagctgg
      721 tgcggctgat ggagaaggtc gggctacggg gcgtgacgta ccggcggctc gggctgggga
      781 ccgtcaccgt ccacaccggg atcgcgtagg cgcgtcgcct accactcggg gctggagtac
      841 tcctcccaca cgcgacgcac gacgcggatc tgctgggcgc ggccgtcttt ctgctcgagc
      901 gtgacgatgt cgcccgcgta gagctgctcg aggtcgcgcg tggtgccttc gtcggtcacc
      961 accagcgtct ctccgcagct caggtcggta cagacctcgg cctcgccgcg cacgcgtacg
     1021 gtgccggcgg ccttgtccac ggagacgatg ctgaagcggt tgtgcttgag ggaatcagtc
     1081 aggctgtaag ccatggcacc ctccttgtcc gacctggctg ctgttatatc aagcggatgg
     1141 ccacccagca agaccttcca ccgggccgcc catgctagta tttttcagaa cttatggcct
     1201 tgaccacccc ccgcggccgt atcgccgaga ccttctattc catccagggc gagggcgcca
     1261 ccgccggcct gccggccgtc ttcgtgcgcc tgcagggctg ctccgtcggc tgcggctggt
     1321 gcgatacgaa gtactcctgg gacccggccg ccggccgcga ggtggacctc cccgcgctcc
     1381 tcgacgaggt ggcggccttc ccctgccgcc gcgtggtgat cacgggcggc gagccactcg
     1441 agtcgccgct cttcacccct ctggcggccg cactcgccgc gcgtgggtac gtcgtcgagg
     1501 tcgagacctc gggcacccgc gcgccggccc cggaagccga cgcgggcatt cagtggaacg
     1561 tctcgctgaa gctgtcgggc tcgggcgttc ccgaggccac ccgcgtcaat ccggccgcga
     1621 tccgcgcctt gctggcacgc ggggcctggt ggaagttcgt ggtgagcgag cccggcgaga
     1681 tcggcgaggc gctccggctg gccgagcgct tcgcgctgcc gcgcgagcgc gtgctgctgc
     1741 agccggaggg cctgcgggcc gaggacctgg cctcgcgcac cccgtggctc gtggaggcct
     1801 gcaaggccca cggattccgc ttctcgcccc ggctccacat cctgatctgg ggcccgaagc
     1861 ggggcgtctg atgccggcgc gccgcgcggt ggtcctgctc tcgggcggcc tcgactcgac
     1921 gaccgcgttc gcggtggcgc gggcggaggg cttcgactgc tacgcgctct ccttcgacta
     1981 cggccagcgg cacgaccggg agctggagtc cgcgcgccgg gtggccgctg ccctcggggc
     2041 caaggagcac ctggtgctgc gcctggacct gcgcgcgatc ggcggctcgg cgctgaccgc
     2101 cgacatcccg gtgcccaagg gccggagcca cgaggccatg ggcacgggca tccccgtgac
     2161 gtacgtcccg gcgcggaaca ccatcttcct gagccacgcg ctggcctggg cggaggtgct
     2221 cgagtcacaa gacatcttcc tgggggtaaa cgccctggat tactcgggct atcccgattg
     2281 ccggcccgag tacatcgagg ccttcgagcg catggcgaac ctcgccacca aggccggcgt
     2341 cgagggccgg agccggctca cgatccacac gcctctgatc cggatgacca aggcccagat
     2401 cgtcaagcgc ggcgccgagc tcggcgtgga cttcggcctc acgtggtcct gctacgagcc
     2461 ccagcccgac ggccgcgcct gcgggctctg cgattcctgc ctcctccggc acaagggctt
     2521 cgccgaggcc ggcctgcgcg atcccgtgcc cgccgcccac tgagcgtccc cgacccgtgt
     2581 ccgatcccgc cgacgttctg agccgcttcc cgcgcgagcg gacctggctg ctacccgccc
     2641 tgcacgcgct ccagcaccgc gatcgctggc tctcgcccgc ggcgctggag gggcggcgga
     2701 ccatctgcgc gtgccgaaga gcgaggtgtt cggcgtcgcc acgcactatc ccgagttccg
     2761 gctcaccgag cctgggcggc gcgtggtgcg ggtctgcgtg gg
//
LOCUS       DASUFA010000087         8183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_707354, whole genome shotgun sequence.
ACCESSION   DASUFA010000087 DASUFA010000000
VERSION     DASUFA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707354"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFX87_03325"
     CDS             <1..822
                     /locus_tag="VFX87_03325"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4993968.1"
                     /translation="PFEAVFVSVKSYDTEWAAQMALAYLKQPDGVVVDFQNGVNDERV
                     ASVVGKSRCLGCVITIGAGMYEPGHAMRTDSGSVGFKIGEHDGRDTPRAQALAKVMND
                     VAGTKVTTNLWGERWSKLAVNCMANPLAGLSGLGSAEVRTAPIPRRIAIHVAAEVIQV
                     GRAAGYEVEPIYGIDARRFVDAAQGKGWDAVEADMAASVKFLTGGRPSMLQDVMKGRR
                     TEIDYLNGYVSQQGKRLGGPTPVNDAVVAAVRRHGVGTLKPDPKNLEPLLAALPW"
     gene            complement(847..2280)
                     /locus_tag="VFX87_03330"
     CDS             complement(847..2280)
                     /locus_tag="VFX87_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4993969.1"
                     /translation="MAGFSDYEQYDALGLADLVRRGKVTPTELLEAAIQRVEARNPKV
                     NAVIMPLYDHGRRAIADGLPDGPFRGVPFLLKDLGAPLTGVTMTRGSRFFADTPPSTF
                     DSENVARLKRAGLVIFGRTNSCEVGLSLTCEPRLYGPTRNPWDPTRISSGSSGGAAAA
                     VGARMLPMAHASDGFGSIRAPAASCGLVGLKPTRGRNTFAPVTGEGLGGCSTEHAVTL
                     SVRDSAALLDATCGPGPGDPYSAPPPARPYLDEVAAAPRPLRIAYTTVTPNGAEVAPE
                     SLATLRDTVRLCTELGHHVEEAHPEIERGAVIPTFITLAAVNTVVNLSTHPTAGRPAR
                     EGEVETVTWNTAKLGERTSSADYVRATQTAHRLGRQMAAFHSRYDVLVTPGLGQLPVK
                     LGWIDMMMDDVEEYWRRVFTFSPFTVWFNLTGQPAMMVPIGQAASGLPVAVQVVARYG
                     DEATLFRLGAQLEAARPWFARKPPLAS"
     gene            complement(2343..3971)
                     /gene="thiC"
                     /locus_tag="VFX87_03335"
     CDS             complement(2343..3971)
                     /gene="thiC"
                     /locus_tag="VFX87_03335"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX4993970.1"
                     /translation="MKIYLSASDPSVRVPFREVALSTGERIRLYDTSGPYTDPGFTAD
                     VKQGLPPLRRHWILSRNDVEPGAGGRWGLRAKPGHRVTQMHYARRGEITPEMEFVALR
                     EGMSPEIVRDEVARGRAIIPSNINHPESEPMIIGRRFLVKINANIGNSAVSSSIEEEV
                     EKMTWATRWGGDTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVNGKAEELT
                     WEIYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRVTGIVSRGGSIMAKWCLAHHQE
                     SFLYTHFREICEIMAAYDVAFSLGDGLRPGCQADANDDAQFAELDTLGELTTVAWEQD
                     CQVMIEGPGHVPMHLIKENMDRQLAVCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMI
                     GWYGTAMLCYVTPKEHLGLPDRQDVKDGVIAYKIAAHAADLAKGHPKARDWDDALSRA
                     RFEFRWEDQFNLSLDPETARSFHDETLPAEGAKVAHFCSMCGPHFCSMKITQDVRDYA
                     AKHGISSETAAVVQGLRDKAAEFKKIGGQLYVKQ"
     regulatory      complement(3962..4074)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4124..4507)
                     /locus_tag="VFX87_03340"
     CDS             complement(4124..4507)
                     /locus_tag="VFX87_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4993971.1"
                     /translation="MDARSPELARAKYIYVTTYSQAGKPGTVPTWLWPHEGAIYFTTR
                     RDSVKARRIRSNGRVSVHVGTKDGPAFEGRAEWVDDRPDLEAALLSIYRRRYWVLVPL
                     FMGRYIRKGLTAKTSVLIRITPLPR"
     gene            4560..6182
                     /gene="ggt"
                     /locus_tag="VFX87_03345"
     CDS             4560..6182
                     /gene="ggt"
                     /locus_tag="VFX87_03345"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4993972.1"
                     /translation="MPPHRHAYRPTVMGTRGVVASAHPLASMAGIRVLLEGGNAVDAA
                     VAVGSTLNVVEPFMSSAGGIGLMFISKARPRERHVLDFIGPAPRAADAARCTEDELAG
                     GPKACATPGNLGGWLAALDRFGSMPRARVLAPAIELAENGVPLTFANVKFFEQARATL
                     GRSAEAGRLYLGNGGPRPGKIVTYKELAATFRQVAEGGAEVFYRGPIAKAIARAVTEA
                     GGWLSEEDLAAFTPEWRQPLSIAYRGREVCSVPPPFSAFQMLETLNILEGYDLAGWGH
                     NSPDYLHQLIEAIKIGSADRLAYAYSGTAPIKGLVSKAYAASQRARIDPARAAVSEGE
                     RHNPERLAGQISEGHPARFENEQTTHFACADAEGMVVSVTQTLGAPFGSGFAVPGTGL
                     VLNNILKWSDRDPASPNVVKPGRKAGTMMSPTQVFQDGAFALSIGTPGSYGILQTQPQ
                     MLLNFLEFGLNLQEAIEAPRIRVYRDRLVDAESRISEATRAELGRRGHQINTIDDWSW
                     VVGGGQGLARDPESGALMAGADPRRDGYALAI"
     gene            6247..7041
                     /locus_tag="VFX87_03350"
     CDS             6247..7041
                     /locus_tag="VFX87_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993973.1"
                     /translation="MVDLQDRVAVVTGAGSGIGRGIAQALAARGARVAAVDIDGASAA
                     ETARLLMGAGGKALGVQADTSAAADVDRAVSTAVGQLGALDIMVNNAGILDGYFNVDE
                     TDDAVWARVIGINLTGVFLGCKRALKEMLPRGRGRIINMASVAGLNGTGGGAAYVASK
                     HGVVGLTRQMAVTYSARGITINAVCPGPILTGLRAHSQQILGPGVPDMSGRGIAVDDA
                     AVRRIAPAGRRGTVEEIASAVCYLASDEAGYITGHSMVVDGGWRAK"
     gene            7038..8183
                     /locus_tag="VFX87_03355"
     CDS             7038..8183
                     /locus_tag="VFX87_03355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4993974.1"
                     /translation="MITKFSVLYVGQIELENVGINGTPTEQRSYPNERLIEAFETSKE
                     VAQLMDELGYYCLWTAEHHFQKEGYEVLPNLVLLSTWLATQTRRLKLGCAFNILPMWH
                     PVRLAEDYAMADIVTNGRVIMGVGRGYHTREVESLGAPLLDAAANRELFEEQMEVLLK
                     CFNEESWSHRGKHYVIPPDVDYRGYRLREVTCVPRPIHRPVEIWMPIASGKSIDYMGR
                     MGLKAMVTLNGEKVLDQVARAYRDACVRHGRPKELGEDLCWGVGLYLADSREEAIRRL
                     EPAHDERYKWFAPFGFVRYADAEGRAWGTPGAPARVPTLREGVEQKAWLVGRPAEVIE
                     TIREIEARYPGLDQCMIHWAEGIPPAEFKEQLRWFARDVMPAFAGRR"
BASE COUNT         1189 a         2771 c         3004 g         1219 t
ORIGIN      
        1 ccgttcgagg cggtcttcgt gtcggtgaag tcctacgaca ccgagtgggc ggcccagatg
       61 gcgctggcct atctcaagca gcccgacgga gtggtggtgg acttccagaa cggggtcaac
      121 gacgagcggg tggcctcggt ggtgggcaag tcgcgctgcc tcgggtgcgt gatcaccatc
      181 ggcgccggca tgtacgagcc cggccacgcg atgcgcaccg actccggcag cgtcggcttc
      241 aagatcggcg agcacgacgg gcgggacacc ccgcgggccc aggcgctcgc caaggtgatg
      301 aacgacgtgg ccggcaccaa ggtgacgacc aacctgtggg gcgagcggtg gtccaagctg
      361 gccgtcaact gcatggccaa cccgctcgcc gggctgagcg gcctgggctc ggccgaggtg
      421 cgcaccgccc ccatcccgcg ccgcatcgcg atccacgtgg ccgcggaagt gatccaggtc
      481 gggcgcgccg cgggctacga ggtcgagccc atctacggca tcgacgcgcg gcgcttcgtg
      541 gacgcggccc agggcaaggg ctgggacgcg gtcgaggccg acatggccgc gagcgtgaag
      601 ttcttgaccg gcgggcggcc gtccatgctc caggacgtca tgaagggacg gcgcaccgag
      661 atcgactatc tgaacggcta cgtgagccag cagggcaagc ggctcggcgg tcccacgccg
      721 gtcaacgacg cggtggtggc cgcggtgcgc cggcacggcg tcggcacgct caagcccgac
      781 ccgaagaacc tcgagccgct cctggccgca ttgccctggt aggcggcggt cagcccgggc
      841 gccggcctac gaggcgaggg gaggcttgcg ggcgaaccag gggcgcgccg cctcgagctg
      901 ggcgcccagg cggaagagcg tggcctcgtc gccgtagcgg gcgacgacct ggaccgccac
      961 cggcagcccg ctcgcggcct gcccgatcgg caccatcatg gcgggctggc cggtgaggtt
     1021 gaaccacacc gtgaacggcg agaacgtgaa cacacgccgc cagtactcct cgacgtcgtc
     1081 catcatcatg tcgatccagc cgagcttgac cggcagctgg cccaggccgg gcgtcaccag
     1141 cacgtcgtag cgcgagtgga aggcggccat ctgccggccc agccggtgcg cggtctgcgt
     1201 cgcgcgcacg tagtcggccg agctcgtgcg ctcgccgagc ttcgcggtgt tccaggtgac
     1261 cgtctccacc tcgccctccc gggcgggccg ccccgcggtg ggatgcgtcg agaggttcac
     1321 caccgtgttg accgcggcca gggtgatgaa cgtggggatc acggcgcccc gctcgatctc
     1381 gggatgggcc tcctccacgt gatgcccgag ctcggtgcag agccgcacgg tatcgcgcag
     1441 ggtcgcgagg ctctcgggcg cgacctccgc cccgttgggc gtgaccgtcg tgtaggcgat
     1501 gcgcagcggc cgcggcgcgg ccgcgacctc gtcgagatag gggcgggcgg gcggcggcgc
     1561 ggagtacgga tcgcccggcc cggggccgca ggtggcgtcg agcagggccg cgctgtcgcg
     1621 caccgagagc gtgaccgcgt gctcggtcga gcagccgccg aggccctcgc cggtcaccgg
     1681 cgcgaaggtg ttgcgcccgc gcgtgggctt gagtccgacc agcccgcagc tcgcggcggg
     1741 ggcgcggatg gagccgaagc cgtcgctggc gtgcgccatc ggcagcatgc gtgcgccgac
     1801 cgcggcggcg gccccgccgc tcgagccgga ggagatccgc gtggggtccc acgggttgcg
     1861 ggtggggccg tagagccggg gctcgcaggt gagcgagagc ccgacctcgc agctattggt
     1921 gcgcccgaag atcaccagcc cggcccgctt gaggcgggcc acgttctcgc tgtcgaaggt
     1981 ggagggcggt gtgtcggcga agaagcggct gccccgcgtc atcgtcaccc cggtcagggg
     2041 cgcgccgagg tccttcagca gaaacggcac gccgcggaac ggcccgtccg gcagcccgtc
     2101 ggcgatcgcc cgccgtccgt ggtcgtagag cggcatgatg accgcgttca ccttcggatt
     2161 gcgcgcctcg acccgctgga tggccgcctc gagcagctcg gtgggcgtca ccttgccgcg
     2221 gcggacgaga tcggccaggc cgagggcgtc gtactgctcg tagtccgaga atcccgccat
     2281 ggtcgctcca cgttcaccct ctcccccgtg agggggagag ggtcgggtga ggggtcctgc
     2341 cgctactgct tcacgtagag ctggccgccg atcttcttga actccgccgc cttgtcccgc
     2401 aggccctgga cgaccgccgc ggtctcgctg gagatcccgt gcttcgccgc gtagtcgcgc
     2461 acgtcctggg tgatcttcat cgagcagaag tgcgggccgc acatcgagca gaagtgcgcg
     2521 accttggcgc cctcggcggg cagggtctcg tcgtggaagg agcgggcggt ctccggatcg
     2581 agcgagaggt tgaactggtc ctcccaccgg aactcgaagc gggcccggga gagcgcgtcg
     2641 tcccagtcac gggccttcgg gtggcccttg gcgaggtcgg cggcgtgggc cgcgatcttg
     2701 taggcgatga cgccgtcctt gacgtcctgc cggtcgggca gccccaggtg ctccttgggc
     2761 gtcacgtagc agagcatggc ggtgccgtac cagccgatca tggccgcgcc gatcgcggag
     2821 gtgatgtggt cgtagcccgg tgcgatgtcg gtggtgagcg ggcccagggt gtagaagggc
     2881 gcctcgtggc agaccgcgag ttgccggtcc atattttcct tgatcaggtg catgggcacg
     2941 tggcccgggc cctcgatcat cacctggcag tcctgctccc acgccaccgt cgtgagctcg
     3001 ccgagcgtgt cgagctcggc gaactgcgcg tcgtcgttgg cgtcggcctg gcagccgggc
     3061 cggagcccgt cgcccagcga gaacgccacg tcgtaggcgg ccatgatctc gcagatctcg
     3121 cggaagtggg tgtagaggaa gctctcctgg tggtgggcca ggcaccactt ggccatgatc
     3181 gagccgccgc gcgagacgat gccggtgacg cgcttggcgg tgagcgggat gtagcgcagc
     3241 agcacgcccg cgtggatcgt gaagtagtcc acgccctgct cggcctgctc gatgagcgtg
     3301 tcgcggtaga tctcccaggt cagctcctcc gccttgccgt tgaccttctc gagggcctgg
     3361 tagatcggca cggtgcccac cggcaccggc gagttgcgca ggatccactc gcgcgtctcg
     3421 tggatgttct tgccggtgga gagatccatg atcgtgtcgc cgccccagcg ggtggcccac
     3481 gtcatcttct ccacctcctc ctcgatcgag gaggagaccg cggagttgcc gatgttggcg
     3541 ttgatcttca ccaggaagcg gcggccgatg atcatcggct ccgactccgg gtgattgatg
     3601 ttggacggga tgatggcgcg gccgcgggcc acctcgtcgc gcacgatctc ggggctcatg
     3661 ccctcgcgca gggccacgaa ctccatctcc ggcgtgatct cgccgcgccg cgcgtagtgc
     3721 atctgggtca cgcgatgacc gggcttggcg cgcaggcccc agcgcccgcc cgcacccggc
     3781 tcgacgtcat tgcgcgagag gatccagtgg cgccgcagcg gcggcaggcc ctgcttgacg
     3841 tccgcggtga agcccgggtc ggtgtagggc ccgctcgtgt cgtagagccg gatccgctcg
     3901 ccggtggaga gcgcgacctc gcggaacgga acgcggaccg acggatcgga tgcggagaga
     3961 tagatcttca tgtcaggcct cccttcgctg gcattaccca ggtcaggttt gtagcggtcg
     4021 gcagcgtgcg gctgccctct cagcccggtc gtccgagctc ccgcgtggga atggctggat
     4081 gaatgctacg tccaaagccc ggggtggtca aggcgccgcg cggtcagcgc ggcaggggcg
     4141 tgatgcggat cagcacgctc gtcttcgcgg tcaggccctt gcggatatac cgccccatga
     4201 acagcgggac cagcacccag taccgccggc gatagatcga cagcagcgcc gcttcgagat
     4261 cgggccggtc gtccacccac tcggcccgcc cttcgaaggc cgggccgtcc ttggtcccca
     4321 cgtgcacgga cacgcgcccg ttgctccgga tgcgccgcgc cttgacgctg tcgcgccggg
     4381 tcgtgaagta gatcgcgccc tcgtgcggcc agagccaggt ggggaccgtg ccgggcttgc
     4441 ccgcctggct gtaggtcgtt acgtagatgt acttggcgcg cgcgagctcg gggcttcttg
     4501 cgtccattcg ggctagagcc taccatgcgg gcaacgactc cagctccgga ggagaccaca
     4561 tgcccccgca ccgtcacgcc taccgcccca ccgtcatggg tacccgcggc gtcgtcgcct
     4621 cggcccatcc gctcgcctcc atggccggca tccgcgtgct gctcgagggc ggcaacgcgg
     4681 tggacgccgc agtcgcggtc ggatccacct tgaatgtcgt cgagccgttc atgtcgagcg
     4741 cgggcggcat cgggctcatg ttcatctcga aggcccgccc gcgtgagcgc cacgtgctgg
     4801 acttcatcgg tccggccccg cgggcggccg acgccgcgcg ctgcaccgag gacgagctgg
     4861 cgggcgggcc caaggcctgc gccacgcccg gcaacctggg cggctggctg gccgcgctcg
     4921 atcgcttcgg gagcatgccg cgggcgcgag tcctggcgcc cgccatcgag ctggccgaga
     4981 acggggtgcc gctgacgttc gccaacgtga agttcttcga gcaggcgcgg gccaccctcg
     5041 gccgctccgc ggaagcgggc cggctctatc tcggcaacgg cgggccccgg ccgggcaaga
     5101 tcgtcacgta caaggagctg gccgcgacgt tccgccaggt cgcggagggc ggcgcggagg
     5161 tcttctaccg cggcccgatc gccaaggcga tcgcccgtgc ggtgacggag gcgggcggct
     5221 ggctctccga ggaggacctg gccgcgttca cgcccgagtg gcggcagcca ctgtccatcg
     5281 cgtatcgggg gcgcgaggtc tgctcggtgc cgccaccctt ctccgccttc cagatgctgg
     5341 agacgctgaa catcctggag ggctacgacc tggcgggatg ggggcacaac tcgcccgact
     5401 atctgcatca gctgatcgag gcgatcaaga tcggctcggc ggaccggctc gcctacgcct
     5461 actccggcac cgcgccgatc aagggcctgg tgtcgaaggc ctacgcggcc tcgcagcgcg
     5521 cacggatcga tccggcgcgg gccgcggtga gcgaggggga gcggcacaac cccgagcggc
     5581 tcgcggggca gatcagcgag ggtcatccgg cgcgcttcga gaacgagcag accacccatt
     5641 tcgcgtgcgc ggacgccgag ggcatggtcg tctcggtgac ccagacgctc ggcgcgccgt
     5701 tcggctcggg cttcgcggtg ccgggcaccg ggctggtgct gaacaacatc ctcaagtgga
     5761 gtgaccggga tccggcctca cccaacgtgg tgaagccggg tcgcaaggcg ggcaccatga
     5821 tgtcgccgac ccaggtgttc caggacggcg ccttcgcgct aagcatcggc acgccgggct
     5881 cctacggcat cctccagacg cagccgcaga tgctgctgaa cttcctggag ttcggcctga
     5941 acctgcagga ggccatcgag gccccccgca tccgcgtcta tcgggaccgg ctggtggacg
     6001 ccgagtcgcg catctccgag gcgacccgcg cggagctcgg ccggcgaggg catcagatca
     6061 ataccatcga cgactggtcg tgggtggtgg gcggcgggca ggggctcgcg cgcgatccgg
     6121 agtcgggcgc cctgatggcc ggcgccgacc cccgccgcga cggctacgcc ctggcaatct
     6181 agaccggggt caggtcttac attcgcacat ttgtcggaat gtaagacctg accccattcg
     6241 gggggaatgg tggatctaca ggacagggtg gcggtggtga cgggcgcggg ttcggggatc
     6301 gggcgcggga tcgcccaggc cctggcggcc cgcggtgcgc gggtggccgc ggtggacatc
     6361 gacggcgcca gcgcggcgga gacggcgcgc ctgctcatgg gcgcgggcgg caaggcgctc
     6421 ggcgtccagg cggacaccag cgcggcggcg gacgtggatc gcgcggtctc gaccgcggtg
     6481 ggccagctcg gcgcgctcga catcatggtg aacaacgcgg gcatcctcga cggctacttc
     6541 aacgtggacg agaccgacga cgcggtctgg gcccgggtga tcgggatcaa cctgaccggc
     6601 gtcttcctcg gctgcaagcg cgccctcaag gagatgctgc cgcgcggccg cggccgcatc
     6661 atcaacatgg cctcggtggc cgggctcaac ggcaccggcg gcggggcggc ctacgtcgcg
     6721 tcgaagcacg gtgtggtcgg gctgacgcgc cagatggcgg tgacctactc ggcccgcggc
     6781 atcacgatca acgcggtctg ccccggaccg atcctgaccg ggctccgggc ccactcccag
     6841 cagatcctgg gcccgggcgt gcccgacatg agcggccgcg gcatcgcggt ggacgacgcg
     6901 gcggtgcgga ggatcgcccc cgccggccgg cgcggaacag tagaggagat cgcctcggcg
     6961 gtctgctatc tggcctccga cgaggcgggc tacatcacgg gacactcgat ggtggtcgac
     7021 ggaggctgga gagccaagtg atcacgaaat tctcggtcct ctacgtgggg cagatcgagc
     7081 tggagaacgt cggcatcaac gggacgccca ccgagcagcg gtcgtatccg aacgagcggc
     7141 tgatcgaggc gttcgaaacg tccaaggagg tggctcagct catggacgag ctcggctact
     7201 actgcctgtg gacggccgag caccacttcc agaaggaagg ctacgaggtg ctgccgaacc
     7261 tcgtgctgct ctccacgtgg ctcgccaccc agacgcggcg cctcaagctc ggctgcgcct
     7321 tcaacatcct gccgatgtgg cacccggtgc ggttggccga ggactacgcc atggccgaca
     7381 tcgtcaccaa cggacgcgtc atcatgggcg tgggccgcgg ctatcacacc cgcgaggtcg
     7441 agtccctcgg ggcgcccctg ctcgacgcgg cggccaaccg ggagctgttc gaggagcaga
     7501 tggaggtgct gctcaagtgc ttcaacgagg agtcctggag ccatcggggc aagcactacg
     7561 tgattccgcc cgacgtggac tatcgcggct atcggctgcg cgaggtcacc tgcgtgcccc
     7621 gtccgatcca ccggcccgtc gagatctgga tgccgatcgc gagcggcaag agcatcgact
     7681 acatgggccg catggggctc aaggccatgg tgacgctcaa cggcgagaag gtgctcgacc
     7741 aggtcgcgcg ggcctaccgc gatgcctgcg tgcgtcacgg gcggcccaag gagctcggcg
     7801 aggatctctg ctggggcgtg ggcctgtacc tggccgactc gcgcgaggag gcgatccgcc
     7861 gtctcgagcc cgcccacgac gagcgctaca agtggttcgc gcccttcggc ttcgtccgct
     7921 acgccgacgc ggagggccgc gcgtggggca cgcccggcgc gcccgcccgc gtgccgacgc
     7981 tccgcgaggg cgtcgagcag aaggcgtggc tggtgggccg tccggccgag gtgatcgaga
     8041 cgatccgcga gatcgaggcc cgctatccgg ggctcgatca gtgcatgatc cactgggcag
     8101 aaggcatccc gcccgccgag ttcaaggagc agctccgctg gttcgcgcgg gacgtgatgc
     8161 cggccttcgc ggggcggcgc tga
//
LOCUS       DASUFA010000088         6503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_708157, whole genome shotgun sequence.
ACCESSION   DASUFA010000088 DASUFA010000000
VERSION     DASUFA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708157"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFX87_03360"
     CDS             <1..828
                     /locus_tag="VFX87_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX4993975.1"
                     /translation="RALDAAREALYQQRRATEAVEPAADPAAEVPTRPQQQADALALL
                     AETALHHGLDPGTPGERYQVVVHVDAAVLADPAQPGQSILEEGPHVSAETSRRLACDA
                     SRVVMRHDADGRVVKVGSRTRTIPPVLRRALQHRDKSCRFPGCSVRGAEGHHVRHWAH
                     GGPTKLSNLALLCRRHHRAVHEEGYQVERGADGTLQFRRPDGRPLPHVPAPPVVPADP
                     AGAIRAQNAAQGLRLHARTGLAQWFGERLDVPWAIDVLHPRARRDSGSSQPRNAPGE"
     gene            complement(839..2911)
                     /locus_tag="VFX87_03365"
     CDS             complement(839..2911)
                     /locus_tag="VFX87_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009032172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX4993976.1"
                     /translation="MAQIPVETVPSVCPHDCTSTCALEVERLDLHTIGRVRGSHRNTY
                     TRGVICEKVGRYAERVHHPDRLRHPLRRVGPKGSGRFERIAWEDALDLVAESFTRQAA
                     RLGLETVWPYYYAGTMGLVQRDGINRLTHAMGYSRYFSTICVTLADTGWAAGVGAKRG
                     VDVREIGPHSDLVVIWGGNPVNTQVNVMSHAMQAKRRGATLVVVDPYRTDTAAKADLH
                     LAPRPGTDGALACAIMHVLFAEGLADWDYLRAYTDCPDDLAAHVATRTPEWAAAITGL
                     SVDQIVSFARLYGRTKKSFIRCHHGFSRSRNGAVNMHAVSCLPAITGAWRHKGGGAVY
                     GQTAIYPLDRTLIEGRDLVDKSIRALDQSRLGQILTGDPEALKGGPPVTAMLVQNTNP
                     AMVCPELNLVHRGLAREDLFLCVHEQFLTETAAFADVVLPATMFVEHDDFYTASGHTY
                     FQVTKKVIEPPGECRENHWVICELAKRLGATHQGFQMTPWEIMDETLRRSGMWDAETN
                     WRRGGQDWAPAFETAHFLDGFPTPDRRFHFKPDWARFGGRGAEMPTLPDHFAVTDEAT
                     TEKPFRLVAAPARTFLNSTFTETPSSLAREKRPTALLHPDDCAALGAVAGTSVQLGNE
                     RGEVIAHAEPREGQPRGVVIVEGVWPNKHFVNRLGINALTSAEPGYPNGGAVFHDTAV
                     WIRRSQPA"
     gene            2946..3377
                     /locus_tag="VFX87_03370"
     CDS             2946..3377
                     /locus_tag="VFX87_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993977.1"
                     /translation="MGTPIRPLPERIARWSTRVDWLRRLDAVAAWLLVWGVAVVRPEV
                     GAGVQAGAAAVAVALLGATPPIRVRWRPVSGPVGLWVSRRLAPGDRAWHVRPEGAELV
                     IVTARRGLRLTVAVPGRGAAEGLSVRRTRVLVFGADEPAPR"
     gene            complement(3457..3660)
                     /locus_tag="VFX87_03375"
     CDS             complement(3457..3660)
                     /locus_tag="VFX87_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993978.1"
                     /translation="MSRFQVMVALGFALSMLVGCEQQGPAQKAGEKVDKAVDQAGKTM
                     QKAGEKVEEAAKEAEKKVKEATK"
     gene            complement(3718..4794)
                     /locus_tag="VFX87_03380"
     CDS             complement(3718..4794)
                     /locus_tag="VFX87_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX4993979.1"
                     /translation="MKIRALRARPVLPPFRRPPVSASGALPKAALVLFDLETDGGITG
                     RSYVFGFTPWTLAAIVGCAQGLFEMIKGDELAPFDLEAKLRRQLTLLDPNGLVGLALA
                     GLDMAAWDALGQARGVPLVTLLGGAPRAIPAYNSTGLWIQPVDRLADEAEALLAEGFS
                     AIKLRVGRDDFAEDLAAVRAVKKRIGDGPTLMCDFNQRLTVNEGLRRARALDGEGLYW
                     IEEPLRHDDYEGYARIAAEVATPIQTGENLVDTFEMARAISLRSLDFVMPDVQRIGGV
                     TGWLRAAALAHAHGIDMSSHLFPEYSVHLLGVTPTGHWLEYMDWAAPLLQEPLRVKNG
                     VAQIPDRPGAGIAWDEDAVKRYAA"
     gene            complement(4808..5239)
                     /locus_tag="VFX87_03385"
     CDS             complement(4808..5239)
                     /locus_tag="VFX87_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993980.1"
                     /translation="MRPRIIPALVVAIGVLATSAVPVPAQTTSTDVSQKASETGQAIK
                     DYTVEKKDEAVAHAKKITADLDAKIKDLEAQASKQTGEIKAKSQAQIKDLKAKRTKAS
                     KKVAELSRATKASWDRAKEGFADAYRDLAAAYDKAVAEFKK"
     gene            5410..6147
                     /locus_tag="VFX87_03390"
     CDS             5410..6147
                     /locus_tag="VFX87_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX4993981.1"
                     /translation="MTTLQRAEAAEHALSQELDRVVVKSVLYTSGERDPRQPVSRPPD
                     DGRLYMMGHDPRLPKMPDKPTLFDFFRLRFGPSAHLLQSARLAVKNGVGEKIVLACLL
                     HDIAIAGFIRCDHGYWSAQLLEPYVDEEVAWGIRYHQALRFFPDESVGYRYPELYTKL
                     FGADYVPEPHIQEAYRYARGHKWYMTSRLITVNDLYAFDPTVQVELEEFTDVVGRHFR
                     QPKEGLGFDQSPSAHMWRTINWPTRYL"
     gene            complement(6274..>6503)
                     /locus_tag="VFX87_03395"
     CDS             complement(6274..>6503)
                     /locus_tag="VFX87_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993982.1"
                     /translation="YVRFYSGDPIRLTAGFYTQYDKQPLFELVERALGHREGVPLPIT
                     PDDPGAPIPYQPGTRGMAGTYAATSLPDQRG"
BASE COUNT          902 a         2229 c         2361 g         1011 t
ORIGIN      
        1 cgggccctgg acgcggcccg cgaggcgctg taccagcagc ggcgggccac cgaggccgtc
       61 gaaccggccg cggacccggc ggccgaggtg ccgactcggc cacagcagca ggcggacgcg
      121 ctggccttgc tggccgagac ggcactgcat cacgggctgg accccggaac cccgggcgag
      181 cggtaccagg tggtcgtcca cgtggacgcg gcggtcctcg cagacccggc acagcccggc
      241 cagtcgatcc tggaggaggg cccgcacgtt tccgcggaaa cgtcgcggcg gctcgcgtgt
      301 gacgcgagcc gggtggtgat gcggcacgat gcggacggcc gggtggtcaa ggtcgggtcg
      361 cgcacgcgga cgattccccc ggtactgcgg cgggcgctgc agcaccggga caagagctgc
      421 cgcttcccgg gctgcagcgt gcggggcgcg gaggggcatc acgtccgcca ctgggcccac
      481 gggggcccga cgaagctgtc gaacctcgcg ttactctgcc gccgccacca ccgcgcggtg
      541 cacgaggagg gctaccaagt cgagcgcggg gccgacggga cgctccagtt ccgacggccg
      601 gatggccggc cattgcccca cgtgccggcg ccgccggtgg tgcccgccga tccagccgga
      661 gcgatcagag cgcagaacgc cgcgcagggg ctccggctcc atgcgcgcac ggggctcgcc
      721 cagtggttcg gggagcgcct ggacgtgccc tgggcgatcg acgtgctgca tccgcgggcg
      781 aggcgagaca gcggctcatc gcagccgcgg aacgcaccgg gcgagtagcc ttccgctcct
      841 aggcaggctg cgagcggcgg atccacaccg cggtgtcgtg gaacacggca ccgccgtttg
      901 gatagccggg ctcggcgctg gtcagggcat tgatgccgag ccggttgacg aagtgcttgt
      961 tcggccacac gccctcgacg atgaccacgc cgcgcggctg gccttcccgg ggctcggcgt
     1021 gggcgatcac ctcgccccgc tcgttgccga gctggaccga tgtgcccgcc accgcgccga
     1081 gggccgcgca gtcatccgga tgcagcaggg cggtcggccg cttctcgcgg gccagggacg
     1141 agggcgtctc ggtgaaggtg gagttgagga acgtgcgcgc gggcgcggcc accagccgga
     1201 agggcttctc ggtggtggcc tcgtcggtca ccgcgaagtg atccgggagc gtgggcatct
     1261 ccgcgccgcg tccgccgaag cgcgcccagt ccggcttgaa gtggaagcgc cggtccggcg
     1321 tggggaagcc gtcgaggaag tgcgcggtct cgaacgcggg tgcccagtcc tggccgcccc
     1381 ggcgccagtt ggtctccgcg tcccacatgc cggaccggcg cagcgtctcg tccatgatct
     1441 cccacggggt catctggaag ccctggtgcg tcgcgccgag ccgcttggcc agctcgcaga
     1501 tcacccagtg gttctcgcgg cactcgcccg gcggctcgat caccttcttg gtcacctgga
     1561 agtaggtgtg cccgctcgcg gtgtagaaat cgtcgtgctc gacgaacatc gtggcgggca
     1621 ggaccacgtc ggcgaaggcc gcggtctcgg tcaggaactg ctcgtgcacg cagaggaaga
     1681 gatcctcgcg cgcgagtccc cggtgcacga gattcagctc cgggcacacc atcgcgggat
     1741 tggtgttctg caccagcatg gcggtgaccg gcgggccgcc cttgagggcc tccggatcgc
     1801 cggtgaggat ctggccgagc cgggactggt cgagggcgcg gatcgacttg tcgacgaggt
     1861 cgcgcccctc gatcagcgtg cggtcgaggg gatagatcgc ggtctggccg tagaccgcgc
     1921 cgccgccctt gtggcgccag gccccggtga tggcgggcag gcaggacact gcatgcatgt
     1981 ttaccgctcc gttgcgggag cggctgaacc cgtggtggca gcggatgaag ctcttcttcg
     2041 tccgcccgta gagccgggcg aagctcacga tctgatccac ggagaggccg gtgatggccg
     2101 ccgcccactc gggcgtccgc gtggccacgt gcgcggccag gtcatcggga cagtcggtgt
     2161 aggcgcgcag gtagtcccag tcggccaggc cctcggcgaa gagcacgtgc atgatcgcgc
     2221 aggcgagcgc gccgtcggtg ccgggccgcg gggccaggtg cagatcggcc ttcgccgcgg
     2281 tgtcggtgcg gtacgggtcc accacgacga gcgtcgcacc gcgccgcttg gcctgcatgg
     2341 cgtggctcat cacgttgacc tgagtgttca ccgggttgcc gccccagatc acgaccaggt
     2401 ccgagtgtgg gccgatctcg cgcacgtcca cgccgcgctt ggcgccgacg ccggcggccc
     2461 agccggtgtc ggccaaggtc acgcagatcg tcgagaagta gcgcgagtag cccatggcgt
     2521 gcgtcagccg gttgatgccg tcgcgctgca ccaggcccat ggtgcccgcg tagtagtacg
     2581 gccacaccgt ctcgagcccg agccgggcgg cctggcgcgt gaagctctcc gcgaccaggt
     2641 cgagcgcgtc ctcccacgcg atgcgctcga agcggcccga gcccttcggg cccacgcgcc
     2701 gcagcgggtg ccgcaggcgg tccggatgat gcacgcgctc ggcgtagcgg ccgaccttct
     2761 cgcagatcac cccgcgcgtg taggtgttgc ggtgcgagcc gcggacgcgg ccgatcgtgt
     2821 ggagatcgag gcgctcgacc tccagggcgc aggtgctggt gcagtcgtgc gggcacaccg
     2881 acgggacggt ctcgacgggg atctgggcca tggccccaag cataccctag agagacaatg
     2941 gggcggtggg gaccccgatc cggccgcttc ccgagcgcat cgcgcgatgg agcacgcgcg
     3001 tggactggct ccgccggctc gatgcggtgg ccgcctggct cctcgtctgg ggggtggcgg
     3061 ttgtccggcc cgaagtcggc gcaggcgtcc aggccggagc ggcggcagtg gccgtcgcac
     3121 tgctcggagc caccccgccg atccgcgtcc gatggcgccc ggtcagcggg ccggtggggc
     3181 tctgggtgag ccggaggctc gcgcccgggg accgtgcctg gcacgtgcgt ccggagggcg
     3241 ccgagctggt gatcgtcacg gcccgccgcg gcctgcgcct gacggtggcg gtgccggggc
     3301 gcggcgccgc cgaggggctc agcgtgcggc ggacgcgcgt gctggttttc ggcgccgacg
     3361 agcccgcgcc ccggtagcta cgtcccctgg gcgtggccgg cgccgtggcg cgttgagcac
     3421 tgctcgcgcg gcgcccccgc cggggactcc gatcgactac ttcgtcgctt ccttgacctt
     3481 cttctctgct tctttcgctg cctcttcgac cttctcgccg gccttctgca tcgtcttacc
     3541 ggcctggtcg accgccttgt ccaccttctc gccggccttc tgggcgggcc cctgctgctc
     3601 gcagcccacg agcatgctga gagcgaagcc cagtgccacc atgacctgaa aacggctcat
     3661 tctcggcatt ggtgtctctc ctccatgaag attgggtccg gcgtgaacgg tatcacctca
     3721 ggccgcatag cgcttgactg cgtcctcgtc ccaggcgatg ccggcgccgg gccggtccgg
     3781 gatctgggcg accccgttct tcacgcgcag gggctcctgc agcaggggcg cggcccagtc
     3841 catgtactcg agccagtgac cggtcggggt caccccgagc aggtgcacgc tgtactcggg
     3901 gaagagatgg ctcgacatgt cgatgccgtg ggcgtgcgcg agggcggccg cgcgcagcca
     3961 gccggtgacc ccgccgatgc gctgcacgtc gggcatgacg aaatcgagcg agcgcagcga
     4021 gatggcgcgc gccatctcga aggtgtccac gaggttctcg ccggtctgga tcggcgtggc
     4081 cacctcggcc gcgatgcgcg cgtagccctc gtagtcgtcg tggcggaggg gctcctcgat
     4141 ccagtagagc ccctcgccgt cgagagcgcg ggcgcgtcga agcccctcgt tcacggtcag
     4201 gcgctggttg aagtcgcaca tgagcgtggg cccgtcgccg atccgcttct tcaccgcgcg
     4261 caccgcggcc aggtcctcgg cgaagtcgtc gcgacccacc cggagcttga tcgcgctgaa
     4321 gccctcggcg aggagcgcct ccgcttcgtc cgcaagcctg tccaccggct ggatccagag
     4381 cccggtgctg ttgtaggcgg gaatggcccg gggcgcgccg cccagcagcg tgaccagcgg
     4441 caccccgcgc gcctggccga gcgcgtccca ggccgccatg tcgagcccgg ccagcgccag
     4501 gccgaccagg ccgttcgggt cgaggagggt gagctgccgg cgcagcttgg cctcgaggtc
     4561 gaagggcgcc agctcgtcgc ccttgatcat ctcgaacagg ccctgcgcgc agcccacgat
     4621 ggcggcgagc gtccacgggg tgaacccgaa gacgtaggag cgcccggtga tgccgccgtc
     4681 ggtctcgaga tcgaagagca ccagcgccgc tttcgggagg gcgccgctgg cgctcaccgg
     4741 gggccggcgg aacggaggca gcacgggccg cgcgcggagg gctcggatct tcatggccgt
     4801 gtcttcacta cttcttgaac tcggccaccg ctttgtcata ggcggccgcc aggtcacggt
     4861 aggcgtcggc gaagccttcc ttcgccctgt cccacgaggc cttcgtggcc cggctcagct
     4921 cggcgacctt cttggatgcc ttcgtccgct tggccttgag atccttgatc tgcgcctgcg
     4981 acttggcctt gatctcgccg gtctgcttgg aggcctgcgc ctcgagatcc ttgatcttgg
     5041 catcgagatc ggcggtgatc ttcttggcgt gcgccaccgc ctcgtccttc ttctccaccg
     5101 tgtagtcctt gatcgcctgg cccgtctccg acgccttctg cgagacgtcc gtggaagtgg
     5161 tctgggccgg aacagggacg gcggaggtgg cgagcacgcc gatcgccacg acaagcgccg
     5221 gaatgattcg tggacgcatg ggcactctct ccttgtcgac agggtggttt tggcctaggc
     5281 gggcgagatg cccgcctggg ccaagtgtaa ggccagcgag tcgggctcgg caatgatggc
     5341 gaacttgaca gccggtgcta ccggggccga tcatcacagg caagactgcc cccagggagg
     5401 gacgccagga tgacgacgct gcagcgggcc gaggctgccg agcacgcgct gagccaggag
     5461 ctcgaccggg tcgtggtgaa gtcggtgctc tacacctccg gcgagcggga tccccgccag
     5521 ccggtgtcgc ggccgccgga cgacggccgg ctctacatga tgggccacga cccccgcctc
     5581 ccgaagatgc ccgacaagcc gaccctgttc gacttcttcc gcctgcgctt cggaccctcc
     5641 gcgcatctcc tgcagagcgc gcggctcgcc gtgaagaacg gagtgggcga gaagatcgtc
     5701 ctggcctgcc tgctgcacga catcgcgatc gccggcttca tccggtgcga ccacggctac
     5761 tggagcgccc agctcctcga gccgtacgtg gacgaggagg tggcctgggg catccgctat
     5821 caccaggcgc tgcggttctt ccccgacgag tcggtgggct accgctatcc cgagctctac
     5881 acgaagctct tcggcgccga ctacgtgccc gagccgcaca tccaggaagc ctaccggtac
     5941 gcgcggggcc acaagtggta catgacctcg cggctcatca ccgtgaacga tctgtacgcc
     6001 ttcgatccca ccgtgcaggt ggagctggag gagttcacgg acgtggtggg gcgtcacttc
     6061 cgccagccga aggaagggct cggcttcgat cagagcccgt cggcgcacat gtggcgcacc
     6121 atcaactggc cgacgcgcta cctctagaag aacagcgtca ggacgttgag gcgctcgtcg
     6181 agcaggtcga gctcctcgag gtaagcgtcg aggtgctggt ggatgaactc ccgccccctg
     6241 tcaatctcag ggtgtgctta ctgcgtgccg agcctagccg cgttgatccg gcaaggaagt
     6301 cgccgcgtac gtcccggcca tgccacgggt gcccggctgg taggggatgg gcgcgccggg
     6361 gtcgtcgggc gtgatcggca gcggcacgcc ctcgcggtgg ccgagcgcgc gctcgaccag
     6421 ctcgaagagc ggctgcttgt cgtactgggt gtagaagccg gcggtcagcc ggatcgggtc
     6481 gccggagtag aagcgcacgt act
//
LOCUS       DASUFA010000089         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_708177, whole genome shotgun sequence.
ACCESSION   DASUFA010000089 DASUFA010000000
VERSION     DASUFA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708177"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFX87_03400"
     CDS             complement(<1..252)
                     /locus_tag="VFX87_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HEX4993983.1"
                     /translation="MTTDTIAELGSIEPNQYGYRDFALGKFSFGRDEYFVHVRWPNGT
                     HMLAVDAFLRAIQRDVAWGFFYGIVNFDTVIGTVNHYGTV"
     gene            complement(257..778)
                     /locus_tag="VFX87_03405"
     CDS             complement(257..778)
                     /locus_tag="VFX87_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HEX4993984.1"
                     /translation="MAPQPTRFGSLQHFEKGGVEVIKDDARNYVFSNVFEVASKAKPY
                     EKVAVGKNIEYVIEAIRAEGTSGWRVAAQDEFALVMDGEVEVRLVKPDNPSLVPPGSQ
                     GSIPLAGDPTGKKMGHVRARRGHMTLLPAGAAYQFHAGHPSVILLQTLAGRDTVERWA
                     EICQTTPRPAQRG"
     gene            complement(735..1250)
                     /locus_tag="VFX87_03410"
     CDS             complement(735..1250)
                     /locus_tag="VFX87_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX4993985.1"
                     /translation="MPTHYRGSTEERAALDAYIKLMRAAESVTARLEPLMRAADLTIG
                     QFGALEALFHLGPLCQRDLGRKLLRSGGNTTVVVGNLARRGLVRRMRRPDDRRFITVT
                     LTDKGRRLIGGMFPSHAQHVVREMRALSLADQAELGRLCRRLGRGLRERREKENGNGA
                     SAHPLRLAPAL"
     gene            complement(1358..2218)
                     /locus_tag="VFX87_03415"
     CDS             complement(1358..2218)
                     /locus_tag="VFX87_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4993986.1"
                     /translation="MRDLEVGRLRVGAVVETAGPTRPTWLFPAATPEGLDRHRAWLAP
                     HFLDTEGRLLQSVHSFVVKAPDLTVLVDTCIGNDKDRGGRRPFHMLRTGFLDTLRAAG
                     VAPEAVDVVICTHLHVDHVGWNTRLDNGRWVPTFPRARYLFARREWEHWTAEDEEGTR
                     RIMADSVAPVLDAGLAELVAMDHRVTDEIRLEPTPGHTPGHVSVRLASGGADAVITGD
                     LMHHPVQIAEPTWQTTFDTDPDAARETRRAFCARHGDRPVTVLGTHFHHPTAGRIVKH
                     GDTWRLAVEA"
     gene            2286..>2869
                     /locus_tag="VFX87_03420"
     CDS             2286..>2869
                     /locus_tag="VFX87_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4993987.1"
                     /translation="MGQLDGKVAIVTGSGRGIGQQVALRLARDGAAVVVNDLDDAPAQ
                     ETISLIEKLGGRAVACNGDVTAKDFGERIVDTAVQKLGGLHVIVNNAGYTWDNVIQKM
                     TDEQWYAIMDVHVTAPFRILRAFAPYLRSRFEAESARGERIVRKVVQVSSTSGVRGNA
                     GQVNYSSAKAAVTGMTRTLAKEWGRYAVTVNCVA"
BASE COUNT          412 a          988 c         1032 g          437 t
ORIGIN      
        1 gacggtgccg tagtggttca ccgtcccgat cacggtgtcg aagttgacga tgccgtagaa
       61 gaagccccac gcgacgtccc gctggatcgc gcggaggaag gcgtccaccg cgagcatgtg
      121 ggtcccgttg ggccagcgga cgtgcacgaa gtactcgtcc cggccgaacg agaacttgcc
      181 cagcgcgaag tcgcggtagc cgtactgatt cggctcgatg ctgcccagct cggcgatcgt
      241 atcggtcgtc atggcgtcat cctctctggg cgggccgggg cgtggtctgg cagatctcgg
      301 cccagcgctc caccgtgtca cggccggcca gggtctgcag caggatcacg ctcgggtgcc
      361 ccgcgtggaa ctggtaggcc gcgccggcgg gcagcagcgt catgtgaccg cgccgcgccc
      421 gcacgtgccc catcttcttg ccggtgggat ccccggccag gggaatcgag ccctggctgc
      481 ccggcggcac cagggacgga ttgtcgggct tgaccaggcg gacctcgacc tcaccgtcca
      541 tcacgagggc gaactcgtcc tgggcggcca cccgccagcc ggacgtgccc tcggcccgga
      601 tcgcctcgat cacgtactcg atgttcttgc cgaccgcgac cttctcgtag ggcttggcct
      661 tcgaggcgac ctcgaagacg ttcgagaaga cgtagttccg cgcgtcgtcc ttgatgacct
      721 cgacgccgcc cttctcaaag tgctggagcg agccgaagcg ggtgggctga ggcgccattg
      781 ccgttctcct tttcgcgacg ctcccggagt ccgcggccga gccgccggca gagccggccg
      841 agctccgcct gatccgccag ggagagcgcg cgcatctcgc gcacgacgtg ttgcgcgtgg
      901 ctgggaaaca tcccgccgat caatcggcgg cccttgtcgg tgagcgtgac ggtgatgaac
      961 cggcggtcgt cgggccgccg catccggcgc acgagcccgc gccgggcgag attgccgacc
     1021 accacggtgg tgttgccgcc gctccgcagg agcttgcgcc cgaggtcgcg ctggcagagc
     1081 ggccccaggt ggaacagggc ctccagggcg ccgaactggc cgatggtcag gtcggccgcc
     1141 cgcatcagcg gctcgaggcg ggccgtcacc gactcggcgg cgcgcatgag cttgatgtag
     1201 gcgtcgagcg ccgccctctc ctccgtactg ccacggtagt gggtaggcat agtttcaact
     1261 tgaaacgttg ttctactacc gccgcgcgcc cgtccttgtc aaggggcgcc cgcgctcctc
     1321 accctctccc ctctggggag agggccgggg tgaggggcta cgcctccacc gcgaggcgcc
     1381 acgtgtcgcc atgcttcacg atgcggcccg ccgtcgggtg gtggaagtgt gtgccgagca
     1441 cggtcaccgg gcgatcgccg tggcgcgcgc agaaggcgcg ccgggtctcg cgcgccgcat
     1501 ccggatcggt gtcgaaggtg gtctgccacg tgggctccgc gatctgcacc gggtggtgca
     1561 tgaggtcgcc ggtgatcacc gcgtcggcgc cgcccgaggc caggcgcacg ctgacgtgcc
     1621 ccggcgtgtg gcccggagtc ggctcgagcc ggatctcgtc ggtcacccgg tggtccatcg
     1681 cgacgagctc ggccaggccc gcgtccagca ccggcgccac gctatcggcc atgatgcgcc
     1741 gcgtgccctc ttcgtcctcg gcggtccagt gctcccactc acggcgcgcg aacaggtagc
     1801 gggcacgcgg gaaggtcggc acccaccgtc cgttgtcgag ccgggtgttc cagccgacgt
     1861 gatccacgtg gagatgcgtg cagatcacca cgtccaccgc ttcgggcgcg acgccggccg
     1921 cccgcagggt gtcgaggaag ccggtgcgca gcatgtggaa tggacgccgg ccgccccggt
     1981 ccttgtcgtt gccgatgcag gtgtccacga gcacggtcag gtcgggcgcc ttcaccacga
     2041 agctgtgcac gctctggagc aggcggccct cggtgtcgag gaagtgcggg gcgagccagg
     2101 cgcggtggcg atcgaggccc tccggcgtgg cggccgggaa cagccacgtg ggacgcgtgg
     2161 ggccggcggt ctcgacgacc gcgcccacgc gcagacggcc gacctcgagg tcgcgcatgg
     2221 ccggcgattg tatcaccgcg aagccccggc gcggcggccg ggcgatcggg tatactccgg
     2281 gcgccatggg acagctcgac ggcaaggtgg ccatcgtcac gggctcgggg cgcggcatcg
     2341 gccagcaggt ggccctgcgg ctcgcgcggg acggcgcggc ggtggtggtc aacgatctcg
     2401 acgacgcgcc cgcccaggag accatctcgc tcatcgagaa gctcgggggc cgggcggtgg
     2461 cgtgcaacgg cgacgtgacc gccaaggact tcggcgagcg catcgtggac accgcggtgc
     2521 agaagctggg cggccttcac gtcatcgtga acaacgccgg ctacacgtgg gacaacgtga
     2581 tccagaagat gaccgacgag cagtggtacg cgatcatgga cgtccacgtg accgccccct
     2641 tccgcatcct ccgcgccttc gccccttacc tccggtcccg gttcgaggcg gagtcggcac
     2701 ggggtgaacg aatcgttcga aaagtcgtgc aggtctcgtc gacctcgggg gtgcgcggca
     2761 acgccggcca ggtcaactac tcttccgcga aggcggcggt gaccggcatg acccgcacgc
     2821 tcgccaagga gtggggccgc tacgcggtca ccgtgaactg cgtggcctt
//
LOCUS       DASUFA010000090         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1510054, whole genome shotgun sequence.
ACCESSION   DASUFA010000090 DASUFA010000000
VERSION     DASUFA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510054"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFX87_03425"
     CDS             <1..621
                     /locus_tag="VFX87_03425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX4993988.1"
                     /translation="DPQAAARFRSPFDLLSPGFGALVTLGMTGEAAWRAGWWWTLLTA
                     IYLHGSLLHLVFNLMWVRQLAPAVEQLFGTARLVVIFTAGGVAGFVLSNVASGTWTVG
                     ASGAIFGLLGAMVAYGRSRGGTFGMAVFRQYGQWAVLMFILGFLMAGVNNWGHAGGFV
                     GGYLTALAVGHGERRSERGVDRVAALATVGLTVVAFALALWNGFLR"
     gene            complement(649..2601)
                     /locus_tag="VFX87_03430"
     CDS             complement(649..2601)
                     /locus_tag="VFX87_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4993989.1"
                     /translation="MIAESIFFRDLAYVFLAAVAGGALARLARQPLILGYVLGGILIS
                     PLTPGPSVSDLHTFELFAEIGVILLMFSIGIEFSLRDLLSVKWVALLGGPLGILLSVG
                     LGVGAAAVLGWPLLPGAIVGIVISVASTMVLARLLMDRGELHSRHGRVMVGITLVEDL
                     AVVILIVLIPALGTLEPGRLLTIAIALGKSLLILIPAIYVAAKVVPPLMTRVARLHSD
                     EMFLLVALAVGLGTAALSQAAGLSLALGAFLAGLIINGSEYAHETLARLLPLRDVFVA
                     LFFVTIGALIDPQSLIQNIPLLAIMVGLIVLGKLVLWTLVVWLFRQPFWTALLVGVGL
                     TQIGEFSFILVQVARNAGHVGAEIYNATLAASLISILINAGLVRTVPGWIARLRGVQE
                     QPAAPGAERAAAEHVVLCGFGRVGSAVGEALETFGIRFVVIERDPDIVRGLRSRAVPC
                     LFGDAAQPRILEEAGSGRASLVVVALPEMDRAQLVVRGVRALSPRVPILARAHSASAR
                     DALSDSGATEVIEPELEAAATLIRHALRKLSLPQPRVLEYLTRFRGAMNPAFADAGAG
                     LKSAEALPEVHEVMLSAGALADLSLGEARVRERFGVTVVSVERPGGQAVVNPSADTRL
                     RPGDRLRVFGLPEQVAAFRHEATRAV"
     gene            2651..>3022
                     /locus_tag="VFX87_03435"
     CDS             2651..>3022
                     /locus_tag="VFX87_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993990.1"
                     /translation="MTERRKTAAARPPAKIKQLEARLAQVEADLARVRSTAARGGLTR
                     ERLAGIEKTMAAQVARAQAGLRDSVNRLSRTLLSARSRKEAAQQIAQARQSVKKSLDR
                     LGQTLGESQKKITREVGLLTRG"
BASE COUNT          487 a         1058 c         1117 g          360 t
ORIGIN      
        1 gacccccagg ccgccgccag gttccggagc cccttcgacc tgctctcgcc gggattcggc
       61 gcgctcgtga cgctcggcat gaccggcgag gcggcgtggc gggcgggttg gtggtggaca
      121 ctcctcaccg cgatctatct ccacgggagc ctgctccacc tcgtcttcaa cctcatgtgg
      181 gtccgccagc tcgcgcccgc ggtcgagcag ctcttcggca ccgcccggct cgtggtcatc
      241 ttcacggcgg gcggcgtcgc gggcttcgtc ctctccaacg tcgccagcgg gacgtggacg
      301 gtcggcgcct cgggcgccat cttcggcctc ctcggtgcga tggtcgccta cggtcgcagc
      361 cgcggcggca ccttcggcat ggcagtgttc cggcagtacg gccagtgggc ggtgctgatg
      421 ttcatcctcg ggttcttgat ggccggcgtg aacaactggg gccacgcggg cggcttcgtg
      481 ggcggctatc tgaccgcgct cgcggtgggt cacggcgagc gccgctcgga gcggggcgtc
      541 gatcgggtgg ccgcgctggc cacggtcggc ctgaccgtgg tggccttcgc cctcgccctc
      601 tggaacggct tcctcagata ggagagcgcc gagaagggcc cgggccggtc agacggcgcg
      661 ggtggcctcg tgacggaacg cggcgacctg ctcgggaagg ccgaagacgc ggagccggtc
      721 acccggccgc aggcgcgtat ccgcggaggg attgacgacc gcttggcccc ccggccgctc
      781 caccgacacc accgtcacgc cgaagcgctc gcgcacccgc gcttcgccga gcgacagatc
      841 ggcgagcgcg cccgcgctca gcatgacctc gtgcacctcg ggcagcgcct ccgcggattt
      901 gagcccggca cccgcgtcgg cgaaggcggg gttcatggcg ccgcggaacc gcgtgaggta
      961 ttcgagcacg cgcggctgcg gcagcgagag cttgcggagg gcatggcgga tcagggtggc
     1021 cgccgcctcc agctccggct cgatgacctc ggtggcgcca gagtccgaca gggcgtcccg
     1081 cgccgaggcg ctgtgcgcgc gcgccaggat gggcacacgc gggctcaggg cccggacccc
     1141 acgcaccacg agctgcgccc gatccatctc gggtagggcc accaccacga gcgacgcccg
     1201 tcccgatccg gcctcctcga ggatgcgcgg ctgcgcggcg tcgccgaaca ggcacggcac
     1261 cgcgcgcgag cggaggcccc gcacgatgtc cggatcccgc tcgatgacga cgaaccggat
     1321 cccgaaggtc tcgagcgcct cgcccaccgc gctgccgacc cggccgaagc cgcagagcac
     1381 gacgtgctcc gccgccgccc gctccgcccc gggggccgcg ggctgctcct ggacgccgcg
     1441 gagacgggcg atccagccgg gaacggtgcg caccagcccc gcgttgatca ggatggagat
     1501 gagcgaggcg gcgagggtgg cgttgtagat ctcggcgccc acgtgaccgg cgttgcgcgc
     1561 gacctgcacc aggatgaagg agaactcgcc gatctgggtc agcccgaccc cgaccagcag
     1621 cgcggtccag aagggctggc gaaagagcca gaccacgagg gtccacagca ccagcttccc
     1681 gagcacgatg agccccacca tgatggcgag gagcgggatg ttctggatga ggctctgggg
     1741 gtcgatcagc gcgccgatgg tgacgaagaa gagcgcgacg aagacgtcgc ggagcggcag
     1801 gagccgggcc agggtctcgt gcgcgtactc cgatccgttg atgatgagcc cggccaggaa
     1861 ggccccgagc gcgagcgaga ggccggccgc ctggctgagg gccgcggtgc cgagcccgac
     1921 cgcgagcgcc acgagcagga acatctcgtc gctgtgcagc cgggccaccc gggtcatcag
     1981 gggcgggacg accttggcgg ccacgtagat ggccgggatc aggatcagca gcgacttgcc
     2041 gagggcgatc gcgatcgtga gcagccgccc gggctccagc gtgcccagcg ccgggatgag
     2101 cacgatgagg atcaccaccg cgagatcctc gaccagcgtg atgccgacca tgacgcggcc
     2161 gtgccgggag tgcagctcgc cacggtccat gagcaggcgc gccagcacca tggtgctggc
     2221 caccgagatc acgatgccca cgatggcccc cggcagcagt ggccagccca ggacggcggc
     2281 cgccccgacg ccgaggccga ccgagagcag gatgccgagc gggccgccga gaagcgccac
     2341 ccacttcacg ctcagcaggt cgcggagcga gaactcgatg ccgatcgaga acatcaacag
     2401 gatgaccccg atctccgcga acagctcgaa ggtgtggaga tcggagaccg acgggccggg
     2461 ggtcagcggg ctgatcaaga taccccccag aacgtagccc aggatgagcg gctggcgggc
     2521 gaggcgggcg agcgccccac ccgcgacggc ggcgaggaac acgtaggcga ggtcgcggaa
     2581 gaagatggat tcggcgatca cagcggggcc tcgcccccga gtatactgct cgcctatgga
     2641 ggaacccccc atgacggagc gacgcaagac cgcggcggcc cgcccgcccg cgaagatcaa
     2701 gcagctcgag gcgcggctgg cccaggtgga agcggatctc gcgcgcgtgc gcagcacggc
     2761 cgcccggggc gggctcaccc gggagcggct ggccggcatc gagaagacga tggccgcgca
     2821 ggtggcccgc gcgcaggccg ggctccgaga ctcggtgaac cggctcagcc ggacgctgct
     2881 cagcgcacgc tcgcgcaagg aggcggcgca gcagatcgcg caggcgcgcc agagcgtcaa
     2941 gaagtcgctc gatcggctcg gccagacgct cggcgagtcc cagaaaaaga tcacgcgcga
     3001 ggtgggcctg ctgacgcgcg gt
//
LOCUS       DASUFA010000091         5970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_21853, whole genome shotgun sequence.
ACCESSION   DASUFA010000091 DASUFA010000000
VERSION     DASUFA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21853"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFX87_03440"
     CDS             <1..434
                     /locus_tag="VFX87_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004413858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4993991.1"
                     /translation="LRERRDDIDPGLVAIADETRSWSPTQFVQAWFDRLAWEEHPRRL
                     FERYDLLLTPTIACAAFKVGLDGPGEIAGTPTGIYDWIPFTYPFNLTGHPGASVPCGF
                     TADKLPIGLQILGRRFEDATVLRASAAFEKIAPWARLRPPV"
     gene            541..1086
                     /locus_tag="VFX87_03445"
     CDS             541..1086
                     /locus_tag="VFX87_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4993992.1"
                     /translation="MDRRADRGYSLVDMLVALAVGGGVLAATVSLLHVGLRAWVWGAA
                     RVEAQQSARYALERMAGELREAGYDPTAAGIAPVVVAEPARVAFQRDMNGNGVIDPTR
                     ERVTYLLRAGESILRRDAGGGAQPVINGVRALRLTYLDREGAETTDPAAVIAVRMQLE
                     VGGDGAGVLMETQATLRNRLW"
     gene            1097..1873
                     /locus_tag="VFX87_03450"
     CDS             1097..1873
                     /locus_tag="VFX87_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX4993993.1"
                     /translation="MRRRFAVTVLLLVALAAPMGCETVPYTGRRQIQLVSSGEEAQMG
                     VQSFKEIVGKATLSTEPQVNALVQRVGGRIAAVTDLKDQWEFRVIQDDKQANAFALPG
                     NKVAVYTGILPITRDEPGLAAVLAHEIGHVLARHGGERLSQQMGVQTVTQLLAGMASS
                     NPATVQLVSAALGAGASVGVLLPWGRAQESEADHLGLILMAKAGYDPRAALDLWKRMA
                     EAAKGQRPPEFLSTHPSEATRIQQIEGWLPEALTYYRPAR"
     gene            2097..2549
                     /gene="mraZ"
                     /locus_tag="VFX87_03455"
     CDS             2097..2549
                     /gene="mraZ"
                     /locus_tag="VFX87_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX4993994.1"
                     /translation="MGGTVFRGHFRHTIDPKGRLSIPAKFRDVLREGGSDRLVMAPNG
                     TSLDVYPEDKWRELEERLNTLPKLDQDRRRFQHVYLSAGLDVVLDPHGRIQILQDYRE
                     RGALAKNVVIIGMMDHFEIWDEDRWSHYQRENAGPLDDLRERLAAKGV"
     gene            2684..3595
                     /gene="rsmH"
                     /locus_tag="VFX87_03460"
     CDS             2684..3595
                     /gene="rsmH"
                     /locus_tag="VFX87_03460"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX4993995.1"
                     /translation="MEPEHAPAAHVPVLLDEVTFLLRPRHGGWVVDGTIGMGGHAEQL
                     LDTAGGESRLLGIDRDPEALARAARRLERFGARVVLVRGSFRHLRAIATNAGVERAAA
                     VLLDLGLSSYQLDESGRGFSFQGEEPLDMRFDPTQGDTAAELLGRLAAEEIAGLLAEY
                     GEERHARRIARVLVEQRRRAPLRTAADLVSVVKRAVPRAAWSKRTHVATRTFQAVRMA
                     VNDEPGALAEALPQAAALLEDGGRLGVISFHSGEDRVVKRAFRSLEESGFVELEPSPV
                     TAGREETAQNPRARSAKLRVLARREAA"
     gene            3592..4056
                     /locus_tag="VFX87_03465"
     CDS             3592..4056
                     /locus_tag="VFX87_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX4993996.1"
                     /translation="MSARAATRSVPQSAWGREHRARQPEAVRRHRQKDRRRLRSMATA
                     VMGAGLAVMLILGVVGLRVQQVRLSYRLDALRGSRAEAEELNRRLRVEMATLKSLSRI
                     EGKARTELGMVAPARDQVRLAREFVPGGKGAVGLDRPSAVAERAVPRDVGVR"
     gene            4066..5901
                     /locus_tag="VFX87_03470"
     CDS             4066..5901
                     /locus_tag="VFX87_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX4993997.1"
                     /translation="MTEIRIEGLRTRTLIFAACLALAFLGLLGRLAYLQVWKHEEYAR
                     LAENQHAKTVPLRPKRGPILDRAGQALAVSSRADTLYVTPVKVEDAGRLAARLAPILG
                     EPARDIVRRLTVSKKFAPVRRRLAPDMARAVRDLKDPSLTLVEDSLRLYPNRELAAQL
                     IGFEGAEGKGLAGMEQVWDAHLAGVEGRAVVERDALGREVTGAPIVLKPSVAGQGIML
                     TIDATIQYLAEKEVDAAWRRTRAKSAMAVAMDPRTGEILAMAIRPTYNPNAFGTATDD
                     DRRVRAVTDPFEPGSTFKVIMAASALEEGVVRPTDRIFGENGAITIANATIHDWKKYG
                     WLTFTEVLQNSSNVGSIKVGMQIGRERYYKYMTGFGFGASTNLGLPGESRGQLRPPAQ
                     WSGLSLATMSIGQEISVTAVQMIAAFSAVANGGRLMQPQIVRAVIDAQGRELRAFEPK
                     TVRQVISPETARTLTEMMVNVVANGTGRHAAIPGYDVAGKTGTAQKLDPATKRYSRAP
                     GVLSFVGFVPADDPRLTMIVLLDEPKNEKWGSEAAAPMFAAIGREALRYLNVPPRDSS
                     PVPIVRGEIVAAASAGPRAGGRRAGRDAASRRPLAPPGHGGPRAL"
BASE COUNT          873 a         2124 c         2134 g          839 t
ORIGIN      
        1 cgctgcgcga gcggcgcgac gacatcgatc cggggctggt cgcgatcgcc gacgagaccc
       61 ggagctggag ccccacccag ttcgtccagg cctggttcga ccgcctcgcc tgggaggagc
      121 acccgcgtcg gctcttcgag cgttacgacc tgctgctcac gccgaccatc gcctgcgcgg
      181 ccttcaaggt cggcctcgac ggccccggtg agatcgcggg cacgcccacc gggatctacg
      241 actggatccc gttcacgtat ccgttcaacc tgaccggcca cccgggcgcc tcggtgccct
      301 gcgggttcac cgccgacaag ctgcccatcg ggctccagat cctgggccgc cgcttcgagg
      361 acgccaccgt gctgcgggcc tcggccgcgt tcgagaagat cgcgccctgg gcccgtctgc
      421 ggccgcccgt ctagggcgcg gtctccgaat cctgacgtct cgaaccgcgg tgtctcccaa
      481 tcttgacgtt ccgaaagatg ggcgttgcgg gcctttggct tcggcggcca gcattggccc
      541 gtggaccggc gcgcggatcg cggctactcg ctggtggaca tgctggtggc cctcgcggtc
      601 ggcggcgggg tgctggccgc caccgtcagc ctgctgcacg tggggcttcg cgcctgggtc
      661 tggggcgccg cgcgcgtcga ggcgcagcag tcagcccgct acgcactcga gcgcatggcg
      721 ggcgagctgc gtgaggccgg gtacgatccc acggcggcgg gtatcgcccc cgtcgtggtt
      781 gccgagccgg cccgcgtggc gtttcagcgg gacatgaacg gaaacggcgt catcgacccg
      841 acccgcgagc gcgtgaccta tctgctgcgg gcgggcgagt ccatcctgcg ccgggacgcg
      901 gggggcgggg cgcagccggt gatcaacggc gtgcgcgcgc tccgtctgac ctatctcgat
      961 cgcgagggcg cggagaccac cgatcccgcc gcggtgatcg cggtgcggat gcagctcgag
     1021 gtcggtggcg acggagcggg cgtgctcatg gagacgcagg ccacgcttcg caatcggctc
     1081 tggtagaatg acggccatgc gtcgccgatt cgccgtcacc gtcctcctcc tcgtggctct
     1141 ggccgccccc atgggctgcg agaccgtgcc ctacacgggc cgccggcaga tccagctcgt
     1201 ctcgtcgggc gaggaagcgc agatgggggt gcagtccttc aaggagatcg tcggcaaggc
     1261 gaccctgtct accgagcctc aggtcaacgc cctggtccag cgggtgggtg gccggatcgc
     1321 cgcggtgacc gatctgaagg accagtggga gttccgcgtc atccaggacg acaagcaggc
     1381 caacgccttc gcgctgcccg gcaacaaggt ggcggtctac accggcatcc tgccgatcac
     1441 gcgcgacgag cccggcctgg ccgcggtgct ggcgcacgag atcggccacg tgctcgcgcg
     1501 tcacggcggc gagcgcctga gccagcagat gggcgtgcag accgtcacgc agttgctggc
     1561 gggcatggcg agcagcaatc cggccaccgt gcagctcgtg agcgccgctc tcggcgccgg
     1621 cgcgtcggtc ggcgtgctgc tgccctgggg tcgcgcgcag gagtcggagg cggaccacct
     1681 gggcctgatc ctgatggcca aggccggcta tgacccgcgg gcggcgctcg acctctggaa
     1741 gcgcatggcc gaggccgcca agggccagcg cccccccgaa ttcctctcga cgcatccctc
     1801 cgaagcgacc cgcatccagc agatcgaggg ctggctgccg gaggctctca cgtactaccg
     1861 cccggcccgt tagggccggc gcccgacccc gaccgtccgc tacccgctgt agccctggtc
     1921 cggccacccc caacccgtgg cccagggctt gggtctgaat ggccttgaaa atcttggagt
     1981 tttcccttga cacccctagc gaccccttct aggatggtcc ccggaagtgg gatgaaatcc
     2041 catgaagtgt cattgaaatg tcatttcagg gtggttccgg gggtagtcag tccccggtgg
     2101 gggggacagt gttccggggg catttcaggc acacgatcga cccgaagggg cgcctcagca
     2161 tcccggcgaa gttccgggac gtgctgcggg agggcggcag cgaccgcctg gtgatggcgc
     2221 ccaacggcac gtcgctcgac gtgtaccccg aggacaagtg gcgcgagctc gaggagcgcc
     2281 tgaacacact gcccaagctc gatcaggatc gccgccgctt ccagcacgtg tacctctccg
     2341 cgggtctcga cgtcgtgctc gatccgcacg gccgcatcca gattctgcag gactatcgcg
     2401 agcgcggcgc gctcgccaag aacgtggtga tcatcggcat gatggatcat ttcgagatct
     2461 gggacgagga tcgctggtcg cactaccagc gcgagaacgc gggcccgctc gacgacctgc
     2521 gcgagcgcct cgccgcgaaa ggcgtctaga aatgcgcaag aagcaacgcc aacggcaaga
     2581 acgcacgaca caccaacggc atgacatcgg agcagtggca gggcgaggtg aagccgaagg
     2641 cgagccgagc gcatttgaaa gagaagcttc cggtggcaac gcgatagagc ccgaacacgc
     2701 accggccgca cacgtaccgg tccttctcga cgaagtgaca ttcctcttgc gcccccgtca
     2761 tgggggttgg gtggtggacg ggacaatcgg catgggcgga cacgcggaac agctgctgga
     2821 cacggcgggt ggcgagagcc gcctgctcgg tatcgatcgc gatcccgaag ccctcgcgcg
     2881 cgcggcgcgc cgcctggagc gcttcggcgc ccgcgtggtg ctggtgcgcg gcagcttccg
     2941 ccacctccgc gcgatcgcga cgaacgcggg cgtggagcgc gcggccgcgg tgctgctgga
     3001 cctggggctg tcgtcgtacc agctcgacga atccggtcgc gggttctcgt tccagggcga
     3061 ggagccgctc gacatgcgct tcgatcccac ccagggcgac accgccgccg agctgctcgg
     3121 ccgcctggcc gccgaggaga tcgcgggcct gctcgccgag tacggcgagg agcggcacgc
     3181 ccggcgcatc gcgcgggtgc tcgtggagca gcgccgtcgc gccccgctgc gcaccgccgc
     3241 cgatctggtc tcggtggtga agcgcgcggt cccgcgcgcc gcgtggtcca agcgcactca
     3301 cgtagccacc cgcaccttcc aggcggtgcg catggcggtc aacgacgagc cgggcgcgct
     3361 cgcggaggcg ctgccgcagg ccgccgcgct cctcgaggac ggcggtcgcc tcggcgtcat
     3421 ctcctttcac tcgggcgagg accgggtggt gaagcgcgcg ttccgctcgc tcgaggagtc
     3481 gggcttcgtg gagctcgagc cctctccggt caccgcgggg cgtgaggaga ccgcccagaa
     3541 cccgcgggcc cgcagcgcca agctgcgcgt gctcgcgcgc cgggaggcag catgagcgcc
     3601 cgggcggcca cccgcagcgt cccccagtcc gcctggggtc gcgagcaccg cgcccggcag
     3661 ccggaggccg tgcggcgcca tcgtcagaag gatcggcgcc ggctccgctc gatggctacc
     3721 gcggtgatgg gcgcgggtct cgcggtgatg ctgatcctgg gcgtggtggg gctgcgagtg
     3781 caacaggtgc gcctgtccta ccggctggac gcgctccgcg gcagccgagc cgaggcggag
     3841 gagctcaatc gccggctgcg ggtggagatg gccacgctga agtcgctctc gcgcatcgag
     3901 ggcaaggccc gcaccgagct cggcatggtg gcgcccgcgc gcgaccaggt ccgcctcgcg
     3961 cgcgagttcg tcccgggcgg caagggcgcc gtcggtctcg accggccgtc ggcggtggcc
     4021 gagcgcgcgg tacctcgtga cgtcggagtg cgctagcggg accgcatgac cgagatccgc
     4081 atcgagggcc tgagaacccg gacgctgatc ttcgcggcgt gcctggccct cgccttcctc
     4141 ggactcctcg gccgcctcgc ctatctgcag gtctggaagc acgaggagta cgcgcggctc
     4201 gccgagaacc agcacgccaa gacggtgccg ctccggccca agcgcggccc gatcctcgac
     4261 cgcgccggcc aggccctcgc ggtctcctcc cgtgccgaca cgctctacgt cacgccggtg
     4321 aaggtagagg acgcgggccg gctcgcggcg cgcctggccc cgatcctggg cgagccggcg
     4381 cgcgacatcg tccgcaggct cacggtctcg aagaagttcg ccccggtacg gcggcgcctc
     4441 gccccggaca tggcgcgcgc ggtgcgcgac ctgaaagacc cttcgctcac cctggtggaa
     4501 gacagcctgc gcctctaccc gaaccgcgag ctcgcggccc agctcatcgg cttcgagggc
     4561 gcggagggca aggggctcgc gggcatggag caggtgtggg acgcgcatct ggccggcgtc
     4621 gaaggccgcg cggtggtgga gcgcgacgcc ctcggccgcg aggtgaccgg cgcacccatc
     4681 gtgctcaagc cgtcggtggc ggggcagggg atcatgctca ccatcgacgc caccatccag
     4741 tacctcgccg agaaggaggt ggacgcggcg tggcggcgca cccgggccaa gtcggcgatg
     4801 gcggtggcga tggacccgcg caccggcgag atcctcgcga tggcgatccg gcccacctac
     4861 aacccgaacg ccttcgggac cgccaccgac gacgaccggc gcgtgcgcgc ggtcaccgac
     4921 cccttcgagc ccggctcgac cttcaaggtg atcatggccg cctccgcgct cgaggagggc
     4981 gtggtgcgcc cgaccgaccg gatcttcggc gagaacgggg cgatcacgat cgccaacgcg
     5041 accatccacg actggaagaa gtacggctgg ctcaccttca ccgaggtgct ccagaactcc
     5101 tccaacgtgg gctcgatcaa ggtcggcatg cagatcggcc gggagcgcta ctacaagtac
     5161 atgaccggct tcggcttcgg ggcctcgacc aacctgggcc tgccgggcga gagccgcggc
     5221 cagctccgtc cgcccgcgca gtggtccggc ctctcgctcg ccaccatgtc catcggccag
     5281 gagatctcgg tgaccgcggt gcagatgatc gccgccttct ccgcggtggc caacggcggg
     5341 cggctcatgc agccgcagat cgttcgcgcg gtgatcgacg cgcaggggcg cgagctccgg
     5401 gccttcgagc ccaagacggt gcgccaggtc atcagccccg agaccgcgcg cacgctgacc
     5461 gagatgatgg tgaacgtggt cgccaacggg accgggcgcc acgcggcgat ccccggctac
     5521 gacgtggcgg gcaagaccgg cacggcccaa aagctggacc cggcgaccaa gcgctactcg
     5581 cgcgcgcccg gcgtgctctc cttcgtggga ttcgtgcccg cggacgaccc gcggctcacc
     5641 atgatcgtgc tgctcgacga gcccaagaac gagaagtggg gcagcgaggc ggcggcgccc
     5701 atgttcgccg cgatcggccg ggaagccctg cgctacctga acgtgccccc gcgcgacagc
     5761 agcccggtgc cgatcgtgcg cggcgagatc gtggccgccg cgtcggccgg cccgcgcgcc
     5821 ggtgggcgac gcgcaggccg tgatgccgcg tctcgccggc ctctcgctcc gccaggccat
     5881 ggaggccctc gcgccctttg acgtgcgcct cgagatcacg ggccacggtg tcgtgaccgg
     5941 ccagacgccg ctgcccggcg ccccgctcgt
//
LOCUS       DASUFA010000092         7759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1389621, whole genome shotgun sequence.
ACCESSION   DASUFA010000092 DASUFA010000000
VERSION     DASUFA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389621"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VFX87_03475"
     CDS             <1..1402
                     /locus_tag="VFX87_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX4993998.1"
                     /translation="ACSEMPARSSMGMEIEASRVPQREAGMTPYEIMLSESQERMLLV
                     AERGREEEVRRIFAKWELDAVEIGTVTDDGLLRVRFHGVVVAEVPVKALADEAPVYEK
                     PTARPGWQDALEAFDPLTLPAPADLSEALLTLLASPGIASKEWVYRQYDQQVGINSLV
                     LPGSDAGVLRIKGTRIGVAVTTDCNARFVYLDPRGGAAMAVAEAARNLSVSGARPLGL
                     TDCLNFGSPERPEILWQFKEAVAGISLACRALEIPVVGGNVSFYNETLGQAVLPTPVI
                     GMAGILDDAEARCTQWFVEAGDRVALLGPDAVSLGGSEWLWVRQQTLAGRLAPLDLSV
                     ERAVQEACRAAIAARLLGSAHDCAEGGLAVALAEACASGPRPLGAEIELGSGGGRADL
                     MLFGEGPSRVVVSVKADRARHFEQLMSEFRVPWRFIGRVGGNRLRIRQGEASLVDLDL
                     NRITSAWRGGFERYVG"
     gene            1392..2819
                     /gene="purF"
                     /locus_tag="VFX87_03480"
     CDS             1392..2819
                     /gene="purF"
                     /locus_tag="VFX87_03480"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX4993999.1"
                     /translation="MSAEATAIELDDDKFHDECGLFGIWNHAEAANVVYLGLYALQHR
                     GQESAGIASTDGDHFQIEKAMGWVADVFSRDRLKRLPGHRAIGHVRYSTAGSSNLRNA
                     QPITGTTVHGPVAIAHNGNLVNAEALREELEKDGAVFQSSSDTEVILHLLARSEGATL
                     AEQLARALTRVRGAYTLLLLTQDSVIGVRDPSGFRPLTLGRLDGAWVLASETCALDLM
                     EAKVERDVEPGEILVIDERGLTSLKPFRAAQRLQCVFEYVYFARPDSMLWGRNVHAVR
                     KALGHQLAREYPVVADLVIAVPDSGVGAALGFSEESGLPYDVGLVRNHYVGRTFIEPQ
                     QGIRHFGVKVKLNPNREVLDGKRVVVSDDSIVRGTTSRKIVKMIRVAGAREVHVRISS
                     PPIQWPCYYGIDTPTRRELIGSTHKVDEIQRYLGADSLGYLSLDGMLKAAGSDPDQFC
                     HACFTGQYQVGFESEELAQLKLFDS"
     gene            2826..3854
                     /gene="purM"
                     /locus_tag="VFX87_03485"
     CDS             2826..3854
                     /gene="purM"
                     /locus_tag="VFX87_03485"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX4994000.1"
                     /translation="MDPLTYRAAGVDIRAGDEVVRRIAPLAASTLRPEVLGGLGAFAA
                     FCRVPFGFQDPVLVSSTDGVGTKLKIAVMADRHDTIGLDLVAMGVNDVVVHGAEPLFF
                     LDYLAVHRVDPEKIEAIVTGIAAGCRQAGCALVGGETAEMGALYAPGEYDLAGFAVGV
                     AERSALITGEHVTAGDRVLGLASSGLHSNGYTLAREAIFGRMGLNLGDPIPGVGRRVG
                     EELLEPTRIYVKPVLELTREVRVRAMAHITGGGITENLPRVLPAQRRARIDPRAWPQP
                     AIFTAIQRAAAVDDAEMRRTFNMGIGFVVVVAPEDAAAATRRLEALGERVYAIGEIVP
                     GAPGVDYV"
     gene            3847..4467
                     /gene="purN"
                     /locus_tag="VFX87_03490"
     CDS             3847..4467
                     /gene="purN"
                     /locus_tag="VFX87_03490"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX4994001.1"
                     /translation="MSEPLRVGVLASGRGSNFRALAEAAAGGRIPAAVVVLVTDRAGA
                     GALTVAGELGIEAVVLEPGHHPSREAHEKAVIAALEERRVGLVCLAGYMRLLSPGFVA
                     HFTGRLLNIHPSLLPAFPGLHPQRQALEHGARVSGATVHFVDDGVDTGPIVLQAAVPV
                     MTDDSEATLADRILVEEHRLYVEAVRLFAQGRLEIRGRRVRIRETP"
     gene            4464..6023
                     /gene="purH"
                     /locus_tag="VFX87_03495"
     CDS             4464..6023
                     /gene="purH"
                     /locus_tag="VFX87_03495"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX4994002.1"
                     /translation="MTRVRRALLSVHDKTGVVEFARGLASLGAEILSTGGTAKLLRES
                     GVSVVDVAEVTGFPEMLDGRVKTLHPGVHGGILARRELPEHLAALERHGIRPIDLVAV
                     TLYPFEQTVARPGVSLEEAIENIDIGGPSMIRGAAKNHAHVAVVTDPGQYGPVLEELR
                     RGDGGLSDATRFRLAAEAFRRTAQYDAAIAAYLREPGATGFPERLPVDGALAQTLRYG
                     ENPHQSAAFYRPTAGALGLSAATQLHGPELSYNNLLDWSAALGLLLEFDDPAAVVIKH
                     TNPCGVALGQSVGEAARKAKACDPVSIYGGIVGVNRTVDLEVVKELSGILLEILFAPA
                     YEADALEEIRRTKKKCRVFQLPCARADYPARMREVRSVWGGVLLQEADLTDLDPASLK
                     VVSKRRPTEAELRGLRFAWRVAKHAKSNAIVLATPDQVVGVGAGQMNRVDSARIAVMR
                     ARELGLETKGSVCASDAFFPFRDGLDVVAKAGATAVIHPGGSVRDEEVVAAADEHGMA
                     MVVCGIRHFKH"
     gene            6202..6933
                     /locus_tag="VFX87_03500"
     CDS             6202..6933
                     /locus_tag="VFX87_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994003.1"
                     /translation="MRQVRAALDGARRLAPGVQTPLAELRLVVRDRQVVVEQDRLRLD
                     PGTGQTLLAFEAGDLERETRETLLTGMVRPLIPPADAAEVWFQRASEWDGDPERWESA
                     VDAYERVLDMDPGYAAAWNNLGLLHHRMGQYERARECYRAALDADESCCQAAFNLGSL
                     HEDLGDFPVAVTWYRRALEMDADYADAHFNLAGVLAKMGRAESAAVHWRRYLELDLGS
                     PWAKIARAHLEEGAEPSGGDAETDE"
     gene            6930..>7759
                     /gene="purD"
                     /locus_tag="VFX87_03505"
     CDS             6930..>7759
                     /gene="purD"
                     /locus_tag="VFX87_03505"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX4994004.1"
                     /translation="MTDVLLVGGGGREHALAWKLAQSPRLGRMVAAPGNPGIAAHARC
                     VAVKDTAIDELVDLARRERPDLVVVGPEVPLAMGLADRMRAAGFAVFGPSAAAARLES
                     SKAFSKDLMARHGVSTGRFRTFQDAAAARGYCRELGAPLVVKADGLAAGKGVTVCRSL
                     EEADRALALCFEERAFGASGLTVVVEEFLEGEEASFFALADGAAVLPFAAAQDHKTVF
                     DDDRGPNTGGMGAYSPAPVMSDAMQDRVMAEIVRPVIAAMGKEGAPYTGVLFVGLMIT
                     R"
BASE COUNT         1033 a         2700 c         2903 g         1123 t
ORIGIN      
        1 cgcctgctcg gagatgccgg cgcgctcgag catgggcatg gagatcgagg cctcccgggt
       61 gccgcagcgc gaggccggga tgacaccgta cgagatcatg ctgtcggagt cgcaggagcg
      121 catgctcctg gtggcggagc gtggccggga ggaagaagtc cgccgcatct tcgccaagtg
      181 ggagctcgac gcggtcgaga tcgggaccgt caccgacgac gggctgctgc gcgtgcgctt
      241 ccacggggtg gtcgtggccg aggtgccggt gaaggccctg gcggacgagg cgccggtata
      301 tgagaagccc accgcccgcc ccgggtggca ggacgctctc gaggccttcg acccgctcac
      361 cctgccggcg cccgccgatc tctcggaggc gctcctgacc ctcctggcct cgcccggcat
      421 cgcctccaag gaatgggtgt accgccagta cgaccagcag gtcgggatca actcgctcgt
      481 gctcccgggc tcggacgcgg gggtgctgcg catcaagggc acccgcatcg gtgtggcggt
      541 aactaccgat tgcaatgcgc gcttcgtgta cctcgatcca cgcgggggtg cggcgatggc
      601 ggtggcggag gccgcgcgta acctgtcggt gagcggggcg cgcccgctcg gactcacgga
      661 ttgcctgaac ttcggctccc ccgagcggcc cgagatcctg tggcagttca aggaagcggt
      721 ggcgggcatc agcctagcct gccgcgcgct ggaaatcccc gtggtaggcg gcaacgtctc
      781 cttctacaat gagaccctcg gtcaggcggt cctccccacc ccggtcatcg ggatggcggg
      841 gatcctcgac gacgcggagg cgcgctgcac tcagtggttc gtggaggccg gggaccgggt
      901 ggccctgctc gggccggacg cggtgagcct gggcggctcg gagtggctgt gggtgcggca
      961 gcagacgctg gccggccggc tcgcgccgct cgacctgtcg gtggagcggg cggtgcagga
     1021 ggcctgtcgc gcggcgatcg cggcgcgcct gctgggctcg gcccacgact gcgccgaggg
     1081 cggcctggcg gtggcgctgg ccgaggcctg tgcgagcgga ccgcgcccgc tgggagcgga
     1141 gatcgagctg ggatcgggcg gcggccgcgc cgatctgatg ctcttcgggg aggggccgtc
     1201 gcgcgtggtg gtctcggtca aggcggaccg agcgcgccac ttcgagcagc tgatgagcga
     1261 gttccgggtg ccgtggcgct tcatcgggcg agtcgggggg aatcggctcc ggatccggca
     1321 gggggaggcg agcctcgtgg acctggacct gaaccgcatc acgagcgcgt ggaggggcgg
     1381 ctttgagcga tatgtcggct gaggcgacgg ccatcgagct ggacgacgac aagttccacg
     1441 acgagtgcgg gctgttcggc atctggaacc atgccgaggc cgccaacgtc gtgtacctcg
     1501 gcctgtacgc gctccagcat cggggccagg aatccgccgg catcgcctcc accgacggcg
     1561 accacttcca gatcgagaag gcgatgggct gggtcgccga cgtgttcagc cgcgaccgcc
     1621 tcaagcgcct ccccggccac cgcgccatcg ggcacgtgcg ctactccacc gcgggcagct
     1681 cgaacctgcg caatgcccag cccatcaccg gcaccaccgt gcacggcccc gtggccatcg
     1741 cccacaacgg caacctcgtg aacgcggagg cgctccggga agagctcgag aaggacggcg
     1801 cggtcttcca gtcgtcgtcc gacaccgagg tcatcctgca cctgctggcc cggtccgagg
     1861 gtgcgacgct ggccgagcag ctggcccgcg cgctcacccg ggtgcgcggc gcctacaccc
     1921 tgctgctgct cacgcaggat tcggtcatcg gcgtgcgtga tccctccggc ttccggccgc
     1981 tcaccctggg tcgcctggac ggggcgtggg tgctggcttc ggaaacctgt gcgctcgacc
     2041 tgatggaagc caaggtggag cgggacgtcg agccgggcga gatcctggtg atcgacgagc
     2101 gcggcctcac ctcgctcaag cccttccgcg ccgcccagcg gctgcagtgc gtgttcgagt
     2161 acgtctactt cgcgcggccg gactcgatgc tctggggccg caacgtgcac gcggtacgca
     2221 aggccctcgg ccaccagctc gcccgcgagt atccggtggt ggccgacctg gtcatcgcgg
     2281 tgcccgactc gggcgtcggg gccgcgctcg gcttctcgga ggaatcgggg ctgccctacg
     2341 acgtgggcct ggtccgcaac cactacgtgg gccgcacctt catcgagccg cagcagggga
     2401 tccgccactt cggggtcaag gtgaagctca acccgaaccg ggaggtcctg gacggcaagc
     2461 gggtcgtggt cagcgacgac tccatcgtcc gcggcaccac gagccgcaag atcgtgaaga
     2521 tgatccgggt cgcaggcgcg cgcgaggtgc acgtgcgcat ctcctcgccg ccgatccagt
     2581 ggccctgcta ctacggcatc gacacgccca cccggcgcga gctgatcggg tcgacccaca
     2641 aggtggatga gatccagcgc tacctcggcg cggactcgct cggctacctg tcgctcgacg
     2701 gcatgctcaa ggccgcgggg tcggatccgg atcagttctg tcacgcgtgc ttcacgggtc
     2761 agtaccaggt cggcttcgag tccgaggaac tggcgcagct caagctgttc gactcgtaag
     2821 gacctatgga tcccctgacg tatcgggccg ccggcgtgga tatccgagcc ggcgacgagg
     2881 ttgtgcgccg gatcgcgccg ctggccgcgt ccaccctgcg ccccgaagtg ctgggcgggc
     2941 tcggagcctt cgccgccttc tgccgcgtcc ccttcggctt ccaggacccg gtgctcgtct
     3001 cctccaccga cggggtgggg accaagctca agatcgcggt gatggcggac cgccacgaca
     3061 cgatcggcct cgatctggtc gcgatgggcg tcaacgacgt ggtggtgcac ggcgccgagc
     3121 cgctcttctt cctcgactac ctggccgtcc accgggttga tccggagaag atcgaggcga
     3181 tcgtgaccgg tatcgcggcg ggctgccgcc aggccggctg cgccctcgtg ggcggcgaga
     3241 ccgccgagat gggggccctc tatgcgccgg gggagtacga cctggccggc ttcgcggtgg
     3301 gcgtggccga gcgctcggcc ctgatcaccg gcgagcacgt caccgcgggc gatcgcgtcc
     3361 tgggcctcgc gtcctcgggc ctgcacagca acggctacac cctggcgcgc gaggcgatct
     3421 tcggccgcat gggcctgaat ctgggcgatc ccatccccgg cgtcgggcgt cgcgtgggcg
     3481 aggagctgct cgagcccacc cgcatctacg tcaagccggt gctcgagctg acccgggagg
     3541 tccgcgtgcg ggcgatggcc cacatcaccg gcggcggaat caccgagaac ctgccgcgtg
     3601 tcctgcccgc ccagcggcgc gcccgcatcg atccccgcgc gtggccccag ccggcgatct
     3661 tcaccgcgat ccagcgcgcc gccgcggtgg acgacgccga gatgcggcgc accttcaaca
     3721 tggggatcgg cttcgtggtc gtggtcgccc ccgaggacgc ggccgccgcc acgcggcgcc
     3781 tcgaggccct gggcgagcgg gtgtacgcga tcggcgagat cgtgccgggc gcgccgggcg
     3841 tggactatgt ctgagccgct gcgcgtgggc gtgctcgcct ccggccgcgg ctccaatttc
     3901 cgcgcgctgg ccgaggccgc cgcgggcggg cgcatccccg ccgcggtggt cgtgctggtg
     3961 acggaccgcg ccggcgcggg cgcgctcacc gtcgcgggcg agctcgggat cgaggcggtc
     4021 gtgctcgagc cggggcatca tccctcgcgc gaggcgcacg agaaggcggt gatcgcagcg
     4081 ctggaggagc gccgtgtcgg cctggtgtgc ctggccgggt acatgcggct cctcagcccc
     4141 ggcttcgtcg cgcacttcac gggccggctc ctcaacatcc acccgtcgct cctcccggcc
     4201 ttcccgggcc tgcacccgca gcgccaggcc ctcgagcacg gggcccgcgt ctccggggcc
     4261 accgtgcact tcgtggacga cggcgtggac accggcccca tcgtgctgca ggcggcggtg
     4321 ccggtgatga cggacgacag cgaagctacc ctcgcggacc ggatcctggt cgaggagcac
     4381 cgcctctacg tagaggccgt ccggctcttc gcacaggggc ggctcgagat ccgcggccgc
     4441 cgcgtgcgga tcagggagac cccatgactc gagtgcgacg cgccctcctg agcgtgcatg
     4501 acaagaccgg ggtggtggag ttcgcccgcg gcctggcatc ccttggcgcc gagatcctct
     4561 ccaccggcgg caccgcgaag ctcctgcgcg agtccggcgt ctccgtggtg gacgtggccg
     4621 aggtcaccgg ctttcccgag atgctcgacg gccgggtgaa gacgctccat cccggcgtgc
     4681 acggcggcat cctggcgcgc cgcgagctgc ccgagcatct ggccgcgctc gagcggcacg
     4741 gcatccggcc gatcgatctc gtggcggtga cgctctatcc cttcgagcag acggtggcgc
     4801 ggccgggcgt gtcgctcgag gaggcgatcg agaacatcga catcggcggg cccagcatga
     4861 tccggggcgc cgccaagaac cacgcccacg tggcggtggt caccgatccc ggccagtacg
     4921 gcccggtgct ggaggagctg cggcgcggcg acggggggct gtccgacgcc acgcgcttcc
     4981 ggctcgccgc cgaggccttc cggcgcaccg cccagtacga cgcggcgatc gcggcctacc
     5041 tgcgcgagcc cggcgcgacg ggatttccgg agcgcctgcc cgtggacggc gcgctcgccc
     5101 agaccctgcg ttacggcgag aacccgcacc agtcggccgc cttctaccgc ccgaccgcgg
     5161 gcgcgctcgg cctgagcgcg gccacccagc tccacggccc cgagctctcg tacaacaacc
     5221 tgctcgactg gtcggccgcg ctcgggctgc tgctcgagtt cgacgatccg gccgcggtag
     5281 tgatcaagca cacgaatccg tgcggcgtgg ccctgggcca gagtgtgggc gaggcggcgc
     5341 gcaaggccaa ggcctgcgat ccggtgtcca tctacggcgg catcgtgggg gtgaaccgga
     5401 cggtggacct cgaggtggtg aaggagctct ccggcatcct gctcgagatc ctcttcgccc
     5461 ccgcctatga ggcggacgcg ctcgaggaga tccgtcgcac caagaagaag tgtcgggtgt
     5521 tccagctccc gtgcgcgcgg gccgattatc cggcgcggat gcgggaggtg cggagcgtct
     5581 ggggcggggt gctgctccag gaggccgacc tcaccgatct cgatccggct tcgctcaagg
     5641 tcgtctcgaa gcgccggccg accgaggcgg agctgcgcgg cctgcgcttc gcctggcgcg
     5701 tggccaagca cgccaagtcc aacgcgatcg tgctggcgac ccccgaccag gtcgtcgggg
     5761 tgggcgccgg gcagatgaac cgggtggact cggcgcgcat cgcggtcatg cgcgcgcgcg
     5821 agctggggct cgagaccaag ggctcggtgt gcgcctccga cgcgttcttc ccgttccgcg
     5881 acgggctcga cgtggtggcc aaggcgggcg ccaccgcggt gatccatccc ggcggctcgg
     5941 tccgcgacga ggaggtcgtg gcggccgccg acgagcacgg catggccatg gtggtgtgcg
     6001 ggattcgcca tttcaagcac tagccctccc ctcgaacggg tcttcacgct gcgggagctg
     6061 acgcggctcc tgcgcctctc gcccaagcgc cgctcgcagc tccggcgcct gggtctgctg
     6121 cgcgacgacc cgggtggcct gcgcttccgc gagctggtct cggcgcgcgc ggccgccaac
     6181 ctgctggaga gcggggcgag cgtgcgccag gtgcgcgccg cgctcgacgg agcccgacgg
     6241 ctcgcccccg gagtgcagac accgctggcc gagctgcgcc tggtggtgcg cgaccgccag
     6301 gtggtggtcg agcaggaccg gctgcggctc gatccgggta ccggccagac gctgctcgcc
     6361 ttcgaggccg gagacctcga gcgcgagacg cgggagacgc tgctgaccgg catggtgcgc
     6421 ccgctcatcc cgcccgccga cgccgccgag gtgtggttcc agcgggcgtc ggagtgggac
     6481 ggcgatccgg agcgctggga gtccgcggtg gatgcctacg agcgcgtgct cgacatggat
     6541 ccggggtacg cggcggcctg gaacaacctg ggcctcctcc atcaccggat ggggcagtac
     6601 gagcgggcca gggagtgcta ccgggccgcg ctcgatgccg acgagagctg ctgccaggcg
     6661 gcgttcaacc tgggctcgct ccacgaggac ctcggcgact tcccggtggc ggtcacctgg
     6721 taccggcgcg cgctcgagat ggacgcggac tacgcggacg cccacttcaa tctggcgggc
     6781 gtgctcgcca agatggggcg ggcggagagc gcggcggtgc actggcggcg gtacctcgag
     6841 ctcgacctgg gcagcccgtg ggccaagatc gcgcgcgcgc acctggagga gggcgccgag
     6901 ccctccggtg gcgacgcgga gaccgacgag tgaccgacgt gctcctggtg ggcggcggcg
     6961 ggcgcgagca cgcgctggcc tggaagctcg cgcagagccc gcgcctgggg cggatggtgg
     7021 cggcgcccgg caatcccggc atcgccgcgc acgcccggtg cgtcgccgtg aaggacaccg
     7081 cgatcgatga gctggtggat ctcgcgcgcc gggagcggcc cgacctggtg gtggtggggc
     7141 ccgaggtgcc gctggcgatg gggctcgcgg accggatgcg cgccgccggc ttcgcggtgt
     7201 tcgggccgag cgcggcggcg gcccgtctcg agagctcgaa ggccttctcc aaggacctga
     7261 tggcccgcca cggcgtgtcc accgggcggt tccgcacgtt ccaggacgcg gcggcggccc
     7321 gtggatactg ccgcgagctg ggcgcgcccc tggtggtgaa ggccgacggg ctcgccgcgg
     7381 gcaagggcgt caccgtgtgc cgctcgctcg aggaggcaga ccgggcgctc gccctctgct
     7441 tcgaggagcg ggctttcggg gcctcgggcc tcacggtggt cgtggaggag ttcctcgaag
     7501 gcgaggaggc ctcgttcttc gcgctggccg acggcgcggc ggtgctgccg ttcgcggccg
     7561 cgcaggacca caagacggtc ttcgacgacg accgggggcc caataccggc ggcatgggcg
     7621 cctactcgcc ggcgccggtc atgagcgacg ccatgcagga tcgcgtcatg gccgagatcg
     7681 tgcggccggt gatcgcggcg atgggcaagg agggcgcgcc gtacaccggc gtgctcttcg
     7741 tcggcctgat gatcacgcg
//
LOCUS       DASUFA010000093         9722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1389644, whole genome shotgun sequence.
ACCESSION   DASUFA010000093 DASUFA010000000
VERSION     DASUFA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389644"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFX87_03510"
     CDS             <1..266
                     /locus_tag="VFX87_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEX4994005.1"
                     /translation="ELRAVATAARDRGWRRVILVTSPFHTRRVQVIWRRESGGAIEGR
                     LATAPDECVAGDPWWRRRRCSEAVLHEYLGLLALYLHISSLMR"
     gene            complement(271..627)
                     /locus_tag="VFX87_03515"
     CDS             complement(271..627)
                     /locus_tag="VFX87_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994006.1"
                     /translation="MPIHTRYLFSAAMDVEPAQERLFNEVYDQEHVPSLLTVPGVIAV
                     SRYEMQPLTMSIGGQLKTIVIEGAPKYGALYEIESPEVLVTEAWAKAVEKGRWPAQVR
                     PHTRNRQHILYRRIGS"
     gene            complement(798..1229)
                     /locus_tag="VFX87_03520"
     CDS             complement(798..1229)
                     /locus_tag="VFX87_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX4994007.1"
                     /translation="MKAPRKRAGETLVGVFETRPWGGFITVEEGRGYKVKRLLVRPGQ
                     RLSLQRHRFRAEHWVVVSGSPRVLVSGRGKRLKPRGAVDVPRGAWHRIENPGRVPVIL
                     IEVQHGPYLGEDDIIRRQDDYGRAGTTPAATAKPSPKRRKR"
     gene            complement(1226..2812)
                     /gene="serA"
                     /locus_tag="VFX87_03525"
     CDS             complement(1226..2812)
                     /gene="serA"
                     /locus_tag="VFX87_03525"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX4994008.1"
                     /translation="MKVLVIDTIAQEGIAYLTERGFEVDQVSSKLPKDELHARLADYE
                     AIVTRSSTAVTPELLGRARRLRILGRAGVGVDNIDVDACSRQGVVVVNAPYGNVVSAA
                     EHTVGMLLALVRKIPAAHDSLKRLEWDRGIYGSELYRKTAGVLGLGKVGSRVAARLRA
                     FDMDILVYDPFIPESRARDLGVRLTDLQTVLSRADILTVHVPLTDETENMIGARELAA
                     LKPGARVINCARGGIVNEVDLLAALDSGHVAGAAVDVWSEEPPRSPAVKRLIQHPRVV
                     VTPHLGANTQEAQVNVAVDVARQLVAFRDGELVEHAVNIPVGDRETMAEQRPFLRLAE
                     LLGRFCVQLEPENVERVDIEVAGNLARVDTELLGRAVLKGLLEGVTAQAVNLVNAHLI
                     ARDRGLVVEVRADAEAASGYTNLITVTTQAGDGRKIIAGTVFDGVPRIVRLRDLYIEF
                     IPEGHILVLAYEDRPGMVGRIGSILGRHNVNIASMHVGRRTRRGRAIVVLLLDDDVPT
                     EVMEEVSKSVEADFARVIRL"
     gene            complement(3028..4446)
                     /gene="hpnJ"
                     /locus_tag="VFX87_03530"
     CDS             complement(3028..4446)
                     /gene="hpnJ"
                     /locus_tag="VFX87_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEX4994009.1"
                     /translation="MTDYLRTLFLHPPAVAGFDGGAGSRYQARREIRSFWYPTWLAQP
                     AALVPGSRLVDAPPDGLTVGDVLPLAKGYDLCVMHTSTPSFASDVRVAEALKAENPAL
                     IIGLVGAAVAVAPEASLRTSAALSFVARNEFDFTIQEVAQGRRFADVRGLSYRVNGHV
                     EHTPERPTLENMDALPFVTPVYKRDLTVEHYAIGYLQHPYVSLYTGRGCRSKCTFCLW
                     PQTVGGQRYRTRSPEHVAEEMAQAARMFPQVREFFFDDDTFTDDLPRAEAIARRLGRL
                     GITWSVNAKANVSYATLKVLKDNGLRLLLVGYESGHQAILNNVKKGVRLDVARRFTRD
                     ARSLGIAIHGTFILGLPGETRETIAETIRFATEIDPDTIQVSLAAPYPGTALYEEAQR
                     NGWLESDELVDRAGVQVSAIGYPHLSRIEIFRSVDQFYRRFYFRPRKMLSLGAEMLRD
                     RQVMKRRLSEGRDFLKFLRSHR"
     gene            complement(4493..5080)
                     /locus_tag="VFX87_03535"
     CDS             complement(4493..5080)
                     /locus_tag="VFX87_03535"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX4994010.1"
                     /translation="MLTELIVHFSYPGVFLVLLATGLGLPIPEELPIVIAAMMSRWEV
                     MQWWGALLSCLGGVLAGDMLLYTVGRHFGRRILEWPAARRILTPEREARVMEAYHRHG
                     LKFVIMARLIMGLRAAAFLTAGLVRVPFLRFLLVDVAAVLVSVPLAFGLAYAVADSVA
                     VALARVHEMQLWIGGGVLLVAAGWLVVLWRRRGQG"
     gene            complement(5084..6883)
                     /locus_tag="VFX87_03540"
     CDS             complement(5084..6883)
                     /locus_tag="VFX87_03540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4994011.1"
                     /translation="MERRGGAGVAVLLVVALAALLLAPGLGRAPFDDPGEGQHAEIAR
                     ELLGGSWLSLRLNGVRYFDKPPLLYWLTAASFHLFGLTEGAARLAPLLGALLAVAGTA
                     LLGVRLLGPGWGALGGLALLSCALFAAFARYVRPESLFVAAIAWGFAGLLVHLRGPGN
                     GPRTPPIADSRSGPVWPVLGCAALGVAALAKDPLGLLGPLVAIVIAAGLAGRLRPVRG
                     WLPPLGVALMVVVGFGWYAVAAVTEPAFLWYTVVDNHVLNVARMRQFPDEDVPLSALE
                     FLTAAGFGAFPWILAAAAAVGALVRARVWRDPAELPWVALAVWAVGLLVFFTVSPFKL
                     PHYGLPAYPALALLAARGWRDVAPRPRGLIAAHLVLFTLAGAGCAWAAAGDGRVFTEA
                     VFGVSDVYSRKEAALGQASPYPAWSSLQPLVAWAAAVLLAGAAVLAAAAVRRSARLGA
                     CAVAAVMLLLVPVVAIGLAVTSSARAVTVMAAEIRARLGPGDLLVLEGPIENAGAVEF
                     YSGHRPVLLDAARSVLGIGATFADAAGTFWSAPRFAEAWASARPPYLLTTREPAQSIV
                     ASLPPRSARLLFAHNGRWLYGRVPAGDAGAAAR"
     gene            complement(6900..7592)
                     /locus_tag="VFX87_03545"
     CDS             complement(6900..7592)
                     /locus_tag="VFX87_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994012.1"
                     /translation="MLVRARGLTKTFRGPDGRPVPVLRGVDVEVKSGEFVAVAGPSGC
                     GKSTLLNLLGLLEPPDAGEIWLDDVCVSRLSRRAQCGVRGRSIGYVFQSFMLIAGLTA
                     LENVLLAARYVGRPRPSALTEARALMDRLGVADRADHYPAQLSGGEQQRVAYCRAVLN
                     APPLVLADEPTGNLDDRHAGVILAELSALCRQRGTSVILVTHRADAAARADRALQMRE
                     GRLESQGGDGHS"
     gene            complement(7608..8798)
                     /locus_tag="VFX87_03550"
     CDS             complement(7608..8798)
                     /locus_tag="VFX87_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994013.1"
                     /translation="MVAIRRLLPRGYLFTVLGIAVGVAGLVALGAMAERITRFIDGGD
                     RFVLGQISVAGEGMGMGTGFTAGGLLRASKIEQIARVPGVAAVQGQVMLPLNPTTSQF
                     LTLTQELILGVDPAVRMSNPEFRELPVRDGRFLRVGDRGVVVVGSDFAASRRLRAGGT
                     LDLATGRTFRVVGVLDKTLTAPDRFAIVPLDDARDLWLRRDPLLVQVFTGGSGGLGRG
                     DLNTGAAVAWVAGVDPDALARRIQAEVPGLNVTIPGELSRLLRESTAFFSALLFGIGA
                     LGLVIGGLSLSNTVTAAVFERIRDFGIKRALGATDGQLLREILGEALGVSISGGLLGV
                     GLALAVGTLVDARGEGQQLFLFSPRLLGFSLVFSIILGGLAATYATLRIVRLSPAEAI
                     RRGA"
     gene            complement(8792..>9722)
                     /locus_tag="VFX87_03555"
     CDS             complement(8792..>9722)
                     /locus_tag="VFX87_03555"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX4994014.1"
                     /translation="HAARQPNVTLLQPATVERVAFDSEGVTAQITDAAGPRELRARFL
                     VDASGRDSFLAARHGHRRPIPGLGKVALFAHFRGGRRWPGLEEGNIRIFIFPAGWFWY
                     IPFADGTTSVGSVLHARTVRGREGDLEQLYDSLILQCRALQEALGDAPRITKVHSAAN
                     FSYRTDPAIGDRFVCVGDALAFVDPIFSSGVYVSSQSAEMASVEILKAFRENRFIEGR
                     FRGYQRRVRKGVGSFQRFIRSFYDPSFIEIFLKPREIVGMVDAVIAVLAGGAFVRRSL
                     KLKLGLEMFFIIVRINRFVRRRRGLTTESRLEW"
BASE COUNT         1515 a         3592 c         3431 g         1184 t
ORIGIN      
        1 cggagctgcg cgcggtggcc acggcggccc gggaccgcgg ctggcgccgc gtcatcctgg
       61 tcacgagccc gttccacacc cgccgcgtgc aggtcatctg gcggcgggag agcggcggcg
      121 ccatcgaggg ccggctcgcg acggcgccgg acgagtgcgt cgccggagac ccgtggtggc
      181 gccgccgccg ctgcagcgag gcggtgctgc acgagtacct gggactcctc gcgctctacc
      241 tccacatctc gagcctgatg cgctgacccg ctaggacccg atgcgacggt agaggatgtg
      301 ctggcgattg cgcgtgtgcg gccgcacctg ggccggccac cggcccttct ccaccgcctt
      361 ggcccacgcc tcggtcacca gcacctcggg gctctcgatt tcgtagaggg caccatactt
      421 tggcgcccct tcgatgacga tggtcttcag ctgcccaccg atgctcatgg tgagcggctg
      481 catctcgtag cgcgagaccg cgatcacacc gggcacggtg agcaggctcg gcacgtgctc
      541 ctggtcgtag acctcgttga agaggcgctc ctgggcgggc tcgacgtcca tggcagcgct
      601 gaacaggtag cgggtgtgga tgggcatggc ggtctcctgt ggtgatgggt tgaagttgac
      661 ccggtcgggg ctctccgctg tgggctgccg ccgtgagctt ctttatctat ccgctcggct
      721 cgccttcggc tgcgcctcgc cgagcaactg cggcgcggtg gttgggccgc cgccggtggg
      781 cggacactcc tcgctctcta gcgcttccgg cgctttggac tcggcttcgc cgtggcggcc
      841 ggggtcgtcc cggcgcggcc gtagtcgtcc tggcggcgga tgatgtcgtc ctcgccgagg
      901 tagggcccgt gctggacctc gatgaggatc accggcacgc ggccggggtt ctcgatgcgg
      961 tgccaggccc cccgcggcac gtccaccgcg ccccggggct tgagccgctt cccgcgaccc
     1021 gagacgagca cccgcgggga gcccgagacc accacccagt gctcggcgcg gaaccggtgc
     1081 cgctgcaggc tcagccgctg gccgggccgg accagcagcc gcttgacctt gtagccgcgg
     1141 ccctcctcca ccgtgatgaa cccgccccag ggccgcgtct cgaagacgcc caccagagtc
     1201 tcgcccgcgc gcttccgggg cgccttcaca accggatcac ccgtgcgaaa tcggcctcca
     1261 cgctcttgct cacctcttcc atgacctccg tgggaacgtc gtcgtccagc agcagcacca
     1321 cgatggcgcg gccccggcgg gtgcgccgcc ccacgtgcat cgaggcgatg ttgacgttgt
     1381 gacggcccag gatcgagccg atccggccca ccatgccggg ccggtcctcg taggccagca
     1441 ccaggatatg cccctcgggg atgaactcga tgtagagatc gcgcagccgg acgatgcggg
     1501 gcaccccgtc gaacacggta cccgcgatga tcttccgccc gtcccccgcc tgcgtcgtga
     1561 ccgtgatcag gttggtgtag ccggaggcgg cctccgcgtc ggcccgcacc tcgaccacga
     1621 gcccgcggtc gcgcgcgatc aggtgggcat tgaccaggtt caccgcctgc gcggtcacgc
     1681 cttcgaggag ccccttcagc accgcgcgac ccagcagctc ggtgtccacg cgggccaggt
     1741 tcccggcgac ctcgatgtcc acccgctcga cgttctccgg ctcgagctgc acgcagaagc
     1801 ggcccagcag ctcggccagg cgcaggaacg ggcgctgctc ggccatggtc tcgcggtcgc
     1861 ccaccgggat gttcaccgcg tgctcgacca gctccccgtc gcggaaggcc acgagctgcc
     1921 gggccacgtc caccgccacg ttgacctgcg cctcctgggt gttggcgccc aggtgcggcg
     1981 tgaccaccac gcggggatgc tggatcaggc gcttgaccgc gggcgagcgg ggcggctcct
     2041 cgctccacac gtccaccgcg gcacccgcca cgtggcccga gtcgagcgcg gccagcaggt
     2101 ccacctcgtt gacgatgccg ccgcgcgcgc agttgatgac gcgcgccccc ggcttgagcg
     2161 cggcgagctc gcgcgccccg atcatgttct cggtctcgtc ggtgagcggc acgtgcacgg
     2221 tcaggatgtc ggcccggctc agcacggtct gcagatcggt cagccgcacc cccaggtcac
     2281 gcgcccggct ctccgggatg aacggatcgt agaccaggat gtccatgtcg aaggcccgca
     2341 gccgcgccgc cacccgcgag cccaccttgc cgagccccag cacgccggcg gtcttgcgat
     2401 agagctcgga gccgtagatg ccccggtccc attcgagccg cttgagcgag tcgtgggccg
     2461 ccgggatctt gcggacgagg gccagcagca tgcccacggt gtgctcggcc gcggagacca
     2521 cgttgccgta gggtgcgttg accaccacca cgccctggcg cgagcaggcg tccacgtcga
     2581 tgttgtccac gccgaccccg gcccgcccca ggatgcgcag ccgccgcgcc cggcccagga
     2641 gctccggcgt caccgcggtg gaggagcgcg tcacgatcgc ctcgtagtcg gccagacgcg
     2701 cgtgcagctc gtccttgggc agcttgctcg agacctgatc cacctcgaac ccgcgctccg
     2761 tcaggtaggc gatgccttcc tgcgcgatcg tatcgatgac gagcactttc atgtaccgct
     2821 catgctagca cgaggatgcg accggcctgg agggcggaca ctcgagcgtc gaggcagacg
     2881 ttccggcgct gtgaccgcgc gcggcgcctg gatgcgggaa gcttctttat ccaatgcggg
     2941 ggcccagaaa tggcccccgc actcccccaa tccgcggaca accgttctcc ctggacagcc
     3001 gtttcggcgc ttggccccgc gcctcgtcta acggtgggag cgcaggaact tgaggaagtc
     3061 gcggccctcg gacaggcggc gcttcatcac ctggcggtcc cggagcatct cggcaccgag
     3121 cgagagcatc ttgcgggggc ggaagtagaa ccggcgatag aactggtcga ccgagcggaa
     3181 gatctcgatg cgcgagaggt gcgggtagcc gatcgcgctc acctgcaccc cggcgcggtc
     3241 caccagctcg tccgactcga gccagccgtt gcgctgcgct tcctcgtaga gcgcggtgcc
     3301 cggatagggc gcggccagcg agacctggat ggtgtcggga tcgatctcgg tggcgaagcg
     3361 gatggtctcc gcgatcgtct cgcgcgtctc gccggggagc cccaggatga aggtgccgtg
     3421 gatggcgatg ccgagcgacc gggcgtcgcg cgtgaaccgc cgagccacgt cgaggcgcac
     3481 gcccttcttg acgttgttca ggatggcctg gtggccggac tcgtagccca ccaggagaag
     3541 ccggagcccg ttgtccttga ggaccttgag cgtcgcgtag gacacgttgg ccttggcgtt
     3601 gaccgaccag gtgatgccga gccgcccgag tcgcctggcg atggcctcgg cccgcggcag
     3661 gtcgtcggtg aacgtgtcgt cgtcgaagaa gaactcgcgc acctgcggga acatccgggc
     3721 ggcctgcgcc atctcctcgg ccacgtgctc ggggctgcgg gtgcggtagc gctgcccgcc
     3781 caccgtctgc ggccagagac agaaggtgca cttggagcgg cagccgcgcc cggtgtagag
     3841 cgacacgtag gggtgctgga ggtagccgat ggcgtagtgc tcgacggtga ggtcgcgctt
     3901 gtagaccggc gtcacgaagg gcagcgcgtc catgttctcg agtgtcgggc gctccggcgt
     3961 gtgctccacg tggccgttga cccggtagga gagcccgcgg acgtcggcga agcgccggcc
     4021 ctgcgccacc tcctggatcg tgaaatcgaa ctcgttccgg gccacgaacg agagcgccgc
     4081 gctcgtgcgg agcgaggcct ccggggccac cgccaccgcc gcccccacga gcccgatgat
     4141 gagcgcgggg ttctcggcct tgagtgcctc ggccacgcgc acgtccgagg cgaacgacgg
     4201 cgtgctggtg tgcatgacgc agaggtcgta gcccttggcg agcggcagca cgtcgcccac
     4261 cgtgaggccg tccggcggag cgtccacgag ccgcgagccc ggcaccagcg cggcgggctg
     4321 ggccagccag gtcgggtacc agaacgaccg gatctcgcgt ctggcctggt agcgcgagcc
     4381 cgccccaccg tcgaagccgg ctaccgcagg ggggtggaga aagagcgtcc gcaggtagtc
     4441 ggtcacgatt ggttcacgat accacagcgg ccggcgcggg cccggacggc cgctagccct
     4501 ggccccgtcg ccgccacagc acgacgagcc atcccgccgc gaccagcaag acgccgccgc
     4561 cgatccagag ctgcatctcg tgcacgcggg cgagcgccac cgccaccgag tccgccaccg
     4621 cgtaggccag gccgaacgcc agcggcacgc tcaccagcac cgcggccacg tccaccagga
     4681 ggaagcggag aaacgggacc cggaccagcc ccgcggtcag gaaggcggcg gcgcgcagcc
     4741 ccatgatgag ccgggccatg atcacgaact tcaggccgtg gcggtgatag gcctccatca
     4801 cgcgggcctc tcgctccggg gtcaggatgc gccgcgccgc cggccactcg aggatgcgcc
     4861 gcccgaagtg ccggcccacc gtgtagagga gcatgtcccc ggccaggacg ccgcccaggc
     4921 aggacaggag ggccccccac cactgcatca cctcccagcg cgacatcatg gcggcgatga
     4981 cgatcggcag ctcctcggga atcggcaggc ccaggccggt ggcgagcagg accaggaaca
     5041 cgcccggata gctgaagtgg acgatcagct cggtgagcat cggtcagcgc gcggccgccc
     5101 ccgcgtcgcc cgcggggacg cggccgtaga gccagcggcc gttgtgcgcg aagagcaacc
     5161 gggcgctccg cggcggcagc gaggccacga tgctctgcgc gggctcccgc gtggtcagca
     5221 gatagggcgg ccgggccgac gcccaggcct ccgcgaagcg cggggcgctc cagaaggtcc
     5281 ccgccgcgtc ggcgaaggtg gcgccgatgc cgagcacgct gcgggccgcg tcaagcagga
     5341 ccggccgatg ccccgagtag aattccaccg cgcccgcgtt ctcgatgggt ccctcgagca
     5401 cgagcagatc accgggcccg agccgcgcgc ggatctccgc ggccatcacc gtgaccgcgc
     5461 gggccgacga ggtcaccgcg agcccgatcg ccaccaccgg caccagcagg agcatcaccg
     5521 ccgccaccgc gcaggcgccc aggcgcgccg agcggcgcac cgccgcggcc gccaggaccg
     5581 ccgcgcccgc gagcaggacc gccgcggccc acgccaccag cggctgcagc gacgaccacg
     5641 cgggataggg cgaggcctgc cccagcgccg cctccttccg cgagtacacg tcggagacgc
     5701 cgaacaccgc ttcggtgaag acccgcccgt cgcccgccgc ggcccacgcg cagcccgcgc
     5761 ccgcgagcgt gaagaggacg agatgcgccg cgatcagccc gcgcggccgc ggcgcaacgt
     5821 cgcgccagcc gcgtgccgcg agcagcgcca gcgcgggata ggcgggcagg ccgtagtgcg
     5881 gcagcttgaa cggcgagacc gtgaagaaga ccaggaggcc caccgcccag acggccaggg
     5941 ccacccaagg cagctccgcg ggatcgcgcc agacgcgcgc gcgaacgagc gcgcccaccg
     6001 ccgccgcagc cgccaggatc cacgggaagg ccccgaagcc cgccgcggtg aggaactcga
     6061 gcgcggagag cggcacgtcc tcgtcgggga actgccgcat gcgcgcgacg ttcagcacgt
     6121 ggttgtccac caccgtgtac cagaggaacg cgggctcggt caccgccgcc accgcgtacc
     6181 agccaaagcc gacgacgacc atgagcgcca caccgagcgg cggcagccac ccccgcaccg
     6241 gccgcagccg cccggccagc cccgccgcga tgacgatggc cacgagcggc ccgagcagcc
     6301 ccagcggatc cttcgccagc gccgcaaccc ccagcgccgc gcacccgaga accggccaga
     6361 ccgggccgga acgactgtcc gcaatcgggg gagtgcgggg gccatttccg ggcccccgca
     6421 gatgaacaag caaccccgca aagccccagg caatggcggc cacgaacagg ctctccggcc
     6481 gaacataccg cgcgaacgcc gcgaacagcg cgcaggacag caacgccagg cccccgagcg
     6541 cgccccagcc gggtcccagc agccggacgc ccaggagcgc agtcccggcc acggcgagca
     6601 gcgcgcccag cagcggcgcc aggcgcgccg cgccctcggt caggccgaag aggtggaagg
     6661 aggccgcggt cagccagtag agcaggggcg gcttgtcgaa gtagcgcacg ccgttcaacc
     6721 gcagcgagag ccagctcccg cccagcagct cgcgggcgat ctcggcgtgc tggccctcgc
     6781 ccggatcgtc gaagggcgcc cggccgaggc ccggggccag cagcaatgcg gccagggcca
     6841 cgaccaggag gacggcgaca cccgcgccgc cgcgccgctc cactagaggc tgcggccgat
     6901 catgagtgcc cgtcgcctcc ttgcgattcg aggcggccct cgcgcatctg gagggcgcgg
     6961 tccgcgcgcg cggccgcatc ggcccgatga gtgaccagga tcaccgacgt cccgcgctgc
     7021 cggcagagcg cgctcagctc ggccaggatc accccggcgt gtctgtcgtc caggttgccg
     7081 gtcggctcgt cggccagcac gagcggcggg gcattgagga ccgcccggca gtaggccacg
     7141 cgctgctgct cgccccccga gagctgcgcc ggatagtgat cggcgcgatc ggcgaccccg
     7201 agccggtcca tcagcgcgcg cgcctccgtc agcgccgacg gccgcgggcg acccacatag
     7261 cgggcggcca gcagcacgtt ctcgagcgcg gtcagcccgg cgatcagcat gaacgactgg
     7321 aagacgtacc cgatggagcg cccccgcacc ccgcactggg cacggcgcga gagccggctc
     7381 acgcacacgt cgtcgagcca gatctcgccc gcgtcgggcg gctcgaggag cccgaggagg
     7441 ttcagcaggg tggacttgcc gcagcccgac ggccccgcca ccgccacgaa ctcccccgac
     7501 ttcacctcca cgtccacgcc gcgaagcacc gggaccggcc ggccgtcggg gccgcggaac
     7561 gtcttggtga gcccgcgcgc gcggacgagc acctcgggcg ccccccgtca ggccccccgc
     7621 cggatggcct cggcgggcga caggcgcacg atccggagcg tggcgtaggt ggccgcgagc
     7681 ccgcccagga tgatcgagaa aacgagcgag aagccgagca ggcgcggtga gaagaggaag
     7741 agctgctggc cctccccgcg cgcgtccacg agcgtgccga ccgcgagggc cagacccact
     7801 cccagcagcc cgcccgagat ggacacgccc agggcctcgc ccaggatctc gcgcaggagc
     7861 tggccgtcgg tggccccgag ggcccgcttg atgccgaagt cgcggatgcg ctcgaagacc
     7921 gccgcggtca ccgtgttgga gagcgagagc ccgccgatga cgaggccgag cgcgccgatc
     7981 ccgaagagca gcgccgagaa gaacgcggtg gattcccgca gcaggcggct cagctccccg
     8041 gggatggtca cgttgaggcc cggcacctcg gcctggatgc gtcgcgcgag cgcgtccgga
     8101 tccaccccgg ctacccacgc caccgccgcc cccgtgttca gatcgccccg gccgaggccc
     8161 ccgctcccgc ccgtgaagac ctggaccagc agcgggtcgc gccggagcca gagatcgcgc
     8221 gcgtcgtcga ggggcacgat cgcgaagcga tcgggcgcgg tgagcgtctt gtcgagcacg
     8281 cccaccaccc ggaacgtccg gccggtcgcc aggtcgagcg tcccgcccgc ccgcaggcgg
     8341 cgcgaggccg cgaagtcgga gccgaccacc accaccccgc gatcgcccac ccgcaggaag
     8401 cgcccgtcgc gcaccgggag ctcgcggaac tcgggattgg acatgcgcac cgcgggatcc
     8461 acgcccagga tcagctcctg ggtgagggtc aggaactgcg acgtggtcgg attgagcggc
     8521 agcatcacct gcccctgcac cgcggccacc ccgggcaccc gcgcgatctg ctcgatcttg
     8581 gacgcgcgca gcaggccgcc cgcggtgaag ccggtgccca tgcccatccc ctcgccggcc
     8641 accgagatct ggcccaggac gaagcggtcg ccgccgtcga tgaagcgcgt gatccgctcg
     8701 gccatggcgc ccagcgcgac caggccggcc acgccgaccg cgatgccgag caccgtgaag
     8761 aggtagcccc gcgggaggag ccggcggatc gctaccattc gaggcgggat tccgtggtca
     8821 ggccccggcg gcgccgaacg aaccgattga tgcgcacgat gatgaagaac atctcgaggc
     8881 ccagcttcag cttgagcgag cggcgcacga aggcgccccc cgccagcacc gcgatgaccg
     8941 cgtccaccat gcccacgatc tcgcgcggct tgagaaagat ctcgatgaag gagggatcgt
     9001 agaagctccg gatgaaccgc tggaaggagc ccacgccctt gcggacgcgg cgctggtaac
     9061 cgcggaagcg gccctcgatg aagcggtttt cgcggaacgc cttcaggatc tccaccgagg
     9121 ccatctccgc cgactgcgac gagacgtaga ccccggagga gaagatcggg tccacgaagg
     9181 cgagggcgtc ccccacgcac acgaaccggt cgccgatggc cgggtcggtc cggtacgaga
     9241 agttggccgc gctgtgcacc ttcgtgatgc ggggggcgtc gccgagcgcc tcctggagcg
     9301 cgcggcactg gagaatcagg gagtcgtaga gctgctccag gtccccctcc cgaccgcgca
     9361 ccgtccgcgc gtggagcacg gagcccacgc tggtcgtccc gtccgcgaac gggatgtacc
     9421 agaaccagcc cgccgggaag atgaagatgc ggatattgcc ctcctccagc ccgggccagc
     9481 ggcggccgcc gcggaagtgg gcgaagagcg cgaccttgcc gagccccggg atcgggcggc
     9541 ggtggccgtg acgggcggcg aggaacgagt cgcgaccgct cgcgtcgacg aggaagcgcg
     9601 cgcgcagctc gcgcgggccg gccgcgtcgg tgatctgcgc ggtcaccccc tcggagtcga
     9661 aggccacgcg ctccacggtg gcgggctgca gcagcgtcac gttcggctgg cgggccgcat
     9721 gg
//
LOCUS       DASUFA010000094         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_938873, whole genome shotgun sequence.
ACCESSION   DASUFA010000094 DASUFA010000000
VERSION     DASUFA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938873"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFX87_03560"
     CDS             complement(<1..393)
                     /locus_tag="VFX87_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994015.1"
                     /translation="MRVRVTDGLVKTDRHTTFYLDAGAPDAPLIVFVHGWPELSLSWR
                     HQLPCFADLGFRAVAPDMRGYGRSGVYARHEDYALEHAVRDMIELLDALGREKAVWVG
                     HDWGSPVVWSLASHHPERCWGVGNLCVPY"
     gene            complement(407..1600)
                     /locus_tag="VFX87_03565"
     CDS             complement(407..1600)
                     /locus_tag="VFX87_03565"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994016.1"
                     /translation="MSKPFAGVRILDFTRYLAGPYGTYQLALLGADVIKIESREGDET
                     RLSPADPKWAERKMAPSFLSMNGNKRSITLDLRRKEAIEIAKRLVVGADVVWENFRGG
                     VMDRLGLGYEALSAINPRLIYCGVSGFGRTGPEKATAAFDGKLQAMSGIMSITGEPAS
                     GPTRAGFALCDTIGGITAALAVASALYQRTHTGRGQLVDVAMLDAALAFIAGPVSEYT
                     VAGVEQRQIGNGSVSRKPTASRFRCGGGYIVLAVLTEKQFVGLMKTLGRPDALADPRF
                     KDWPARTANEPALREIIEGALASDDPRTWEARLTAADVPCGSIWKVDEIVDHPQLKHR
                     DVLQTIDSRYGPMRLVGAGFRLEHGSPGIDREPPTLGEHTDEILAEAGYTAEEIERLR
                     HDAVV"
     gene            complement(1597..2529)
                     /locus_tag="VFX87_03570"
     CDS             complement(1597..2529)
                     /locus_tag="VFX87_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1984..1986),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HEX4994017.1"
                     /translation="MSIGSPGSKVAFAPDDDVAIQYMQRTRDYYQALGFAPYRWAHFT
                     DAPFTPLSMPLARARVALITTAAPLQPEAGDQGPRAPYNAAAKFYQVYSAPVDSKPDL
                     RISHVGYDRANTVPEDLDAYFPLCRLQEAVAAGRIGGLTPRFHGAPTNRSHRVTVETD
                     APELLRRCREDGADAAVLVPTUPICHQTVGLAARHLEANGIPTVIMGCAKDIVEHCGV
                     PRFVWSDFPLGNSAGRPFDVASQTQTLDLALRLLEAAPAARTTVASPLRWAPDAAWKH
                     RYLDVGKLSPEEIAERRKEFDVVKAVAKAKREGA"
     gene            complement(2526..>3293)
                     /locus_tag="VFX87_03575"
     CDS             complement(2526..>3293)
                     /locus_tag="VFX87_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX4994018.1"
                     /translation="FDRLGLDYVAAGGPTYCCGIQHHRRGQEASGERYANHTVELLQS
                     MAPREVVMWCPSCIQFYDEVLQADLPFPKRHTAEFLADCLDRGLFTFTQRVEATVALH
                     YHYATEARAREGRAARRLLEAVPGITVIEFEPDARWGRTCTPALQEQLGPAVWRQMAL
                     EDIDRALGAGATHLAGIYHGCHRDQCRFERERPIVVEHYLTLFARALGIEFEDTYKKY
                     MHMGDPDRILEDASPCMAANGVDPQKARGLVIKTFSA"
BASE COUNT          456 a         1177 c         1174 g          486 t
ORIGIN      
        1 atagggcacg cagaggttgc ccacgcccca gcatcgctcc gggtggtggc tcgccaggct
       61 ccagacgacc gggctgcccc aatcgtggcc gacccacacc gccttctccc ggcccagcgc
      121 gtccagcagc tcgatcatgt cccgcaccgc atgctcgagc gcatagtcct cgtggcgcgc
      181 gtagacgccg gagcgaccgt agccccgcat gtccggggcg accgcgcgga acccgaggtc
      241 ggcgaagcac gggagctggt gccgccagct caacgacagc tccggccagc cgtggacgaa
      301 cacgatgagg ggcgcgtcgg gcgcgccggc atcgagatag aacgtggtgt gccggtccgt
      361 cttcacgagg ccgtccgtca cccggaccct cacgcgccgc tccccgtcac accaccgcgt
      421 cgtgccggag ccgctcgatc tcctccgccg tgtagccggc ctcggccagg atctcgtcgg
      481 tgtgctcgcc gagggtcggc ggctcccggt cgatccccgg gctgccgtgc tcgagccgga
      541 atccggcgcc cacgagacgc atcgggccgt agcgggaatc gatcgtctgg agcacgtcgc
      601 ggtgcttgag ctgcgggtgg tcgacgatct cgtccacctt ccagatcgag ccgcagggca
      661 cgtccgctgc ggtgaggcga gcctcccagg tgcgcggatc gtcgctggcg agcgccccct
      721 cgatgatctc gcggagcgcg ggctcgttgg cggtgcgggc cggccagtcc ttgaagcgcg
      781 ggtcggcgag cgcgtcgggg cgaccgagcg tcttcatcag gccgacgaac tgcttctcgg
      841 tgagcacagc caggacgatg tacccaccgc cgcagcggaa ccggctcgcg gtgggcttgc
      901 ggctcaccga cccgttgccg atctggcgct gctcgacgcc ggccaccgtg tattccgaca
      961 ccggccccgc gatgaaggcc agcgcggcgt ccagcatcgc cacgtcgacg agctgcccgc
     1021 ggccggtgtg cgtgcgctgg tagagcgcgc tcgcgaccgc gagcgccgcg gtgatgccgc
     1081 cgatggtgtc gcagagcgcg aagccggcgc gcgtgggacc cgaggccggc tcgccggtga
     1141 tggacatgat cccggacatc gcctgcagct tgccgtcgaa ggccgcggtg gccttctccg
     1201 gcccggtgcg gccgaagccg gagacaccgc agtagatgag ccgcggattg atcgcggaga
     1261 gggcctcgta gccgagcccg aggcggtcca tcaccccgcc ccggaagttc tcccagacca
     1321 cgtcggcccc gaccaccagc cgcttggcga tctcgatcgc ttccttgcgc cggaggtcga
     1381 gcgtgatgga ccgcttgttg ccgttcatgg agaggaagga cggcgccatc ttccgctccg
     1441 cccacttggg atcggcgggg gagaggcgcg tctcgtcgcc ctcgcgcgac tcgatcttga
     1501 tgacatcggc gccgaggagg gcgagctggt acgtcccgta cgggccggcg agatagcgcg
     1561 tgaagtccag gatgcggacg ccggcgaacg gcttgctcac gcgccctcgc gcttggcctt
     1621 cgccaccgcc ttcaccacgt cgaactcctt ccgccgctcc gcgatctcct ccggcgagag
     1681 tttcccgacg tcgaggtacc tgtgcttcca cgccgcgtcc ggggcccagc ggagcggcga
     1741 cgccaccgtg gtccgcgcgg ccggcgccgc ctcgagcagc cgcagggcca ggtcgagcgt
     1801 ctgggtctgc gacgccacgt cgaacggtcg ccctgcggaa ttgccgagcg ggaagtcgct
     1861 ccacacgaag cgcgggaccc cgcagtgctc cacgatgtcc ttggcgcagc ccatgatcac
     1921 ggtcgggatc ccgttcgcct ccagatgccg ggcggccaga cccacggtct ggtggcagat
     1981 cggtcaggtc ggaaccagga ccgccgcgtc ggcgccgtcc tcccggcaac ggcgcagcag
     2041 ctccggcgcg tccgtctcca cggtcacgcg gtggctccgg ttcgtcggcg ccccgtggaa
     2101 gcgcggggtc agccccccga tccgtcccgc cgcgacggct tcctggagcc ggcacagcgg
     2161 gaagtaggcg tcgaggtcct cgggcaccgt gttggcccgg tcgtagccca cgtgcgagat
     2221 gcggagatcg ggcttgctgt ccaccggggc ggagtacacc tggtagaact tcgcggccgc
     2281 gttgtagggg gcgcgtggcc cctggtcgcc ggcctccggc tgcaagggcg ccgcggtcgt
     2341 gatcagcgca acccgggccc gcgccagcgg catggagagc ggtgtgaacg gcgcgtccgt
     2401 gaagtgggcc cagcggtacg gggcgaagcc gagcgcctgg tagtagtcgc gcgtgcgctg
     2461 catgtactgg atcgccacgt cgtcgtccgg cgcgaacgcg accttggacc caggcgaccc
     2521 gatgctcatg ccgagaaggt cttgatgacg agcccgcgcg ccttctgggg atcgacgccg
     2581 ttggccgcca tgcaggggct ggcgtcctcc aggatccggt cggggtcacc catgtgcatg
     2641 tacttcttgt aggtgtcctc gaactcgatg ccgagcgcgc gcgcgaagag cgtgaggtag
     2701 tgctcgacca cgatgggccg ctcccgctcg aaccggcact gatcgcgatg acagccgtgg
     2761 tagatgccgg ccaggtgcgt ggctcccgcc ccgagcgccc ggtcgatgtc ctcgagcgcc
     2821 atctgccgcc agaccgcggg cccgagctgc tcctggagcg ccggcgtgca cgtgcgcccc
     2881 cagcgcgcgt cgggctcgaa ctcgatgacc gtgatgccgg gcaccgcctc aagcagccgc
     2941 cgtgcggccc gcccttcgcg cgcccgagcc tcggtcgcgt agtggtagtg cagcgccacg
     3001 gtggcctcga cccgctgcgt gaacgtgaag agcccgcggt cgaggcagtc ggcgaggaac
     3061 tcggcggtgt gccgcttcgg gaacggcaga tcggcctgga gcacctcgtc gtagaactgg
     3121 atgcaggacg ggcaccacat cacgacctcg cgcggcgcca tgctctgcag cagctccacg
     3181 gtgtggttgg cgtagcgctc gcccgaggcc tcctgcccgc gccggtggtg ctggatgccg
     3241 cagcagtagg tcggcccgcc cgcggccacg tagtcgagcc cgagccggtc gaa
//
LOCUS       DASUFA010000095         8008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_136944, whole genome shotgun sequence.
ACCESSION   DASUFA010000095 DASUFA010000000
VERSION     DASUFA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136944"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFX87_03580"
     CDS             <1..732
                     /locus_tag="VFX87_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994019.1"
                     /translation="TDYVTRAARGDLGVSLRSNLPVAALIRERLPATVELTVAALLLA
                     VVVAIPAGIVSAVKRGSALDRLAMVGAVAGQAVPIFWLALLLIAFFGVYLRWLPVFGR
                     GSPAHLVLPAVSLSTVILGRLARLVRSSMLEVLGQDYVRTARAKGVGEVRVLAGHALK
                     NAAIPIVTLLGLQFAQLLGGAVVTETIFAWPGIGRLVVEAIFNRDFPVVQGVVLVVSL
                     IFVAVNLLVDLAYAALDPRIRTEAA"
     gene            732..1565
                     /locus_tag="VFX87_03585"
     CDS             732..1565
                     /locus_tag="VFX87_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994020.1"
                     /translation="MRRDWAFWLSAAALAIIVLAAVFAEWLAPYGPNEQDILTRLQPP
                     DLSAHVLGTDEVGRDILSRLIHGARISLLVGVIAVGFSCPLGVRVGLVAGYAGRRVDS
                     VLMHVTDIQLAIPTILLAIAVVAVLGPGLPNVILTLSVTGWTLYARLVRGETLGVKTR
                     DFVQAARANGAGDARIMTRHVLPNVLSPVIVVAVFAVANMIVLEATLSFLGLGVEPSV
                     VTWGRMLNGGRLYLNTAWWLTAFPGLAIFVTVLAVNLLGDHLRDWLDPRLRGSLGSAQ
                     A"
     gene            complement(1569..3401)
                     /locus_tag="VFX87_03590"
     CDS             complement(1569..3401)
                     /locus_tag="VFX87_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4994021.1"
                     /translation="MPEPPTSEIPPVDPITLEIIRGSLASAIRDMELLMERCAMSPFI
                     KEKKDYFVGIFDTRGRIVACHISGSGPGMLQPILTLYPLETMRPGDVYWFNDPYISDG
                     AVQHHQDMVFTVPVFHEGRPVAFATTFGHYQDIGGLRAGSISPHATEIYHEGVLVPPV
                     RIVREGQLNEEAYRIFLRNSRLPDLVEGDTRAMMASCHLAETRLGELCARYGVDTLLA
                     AFDECIAQTAVRARELFLLLVPPGEWRFHDFLDSDGGTEARPYRIDLTLTRVGDRVRL
                     DGSNSDDQARGPINFTTNPGLLRIAFGRYLQSLDADLEVNEGLLHNLDEWIAREGSIL
                     KPRFPAPLGMRANTRFRVMSCIFGTLAQANGGRVPAGSPVYVLYYFRSWDAARQRPIL
                     CIEGLGVGLGARPFADGVDVIYYIAQENYPVEYVERDFPLRVERYAVRPDSGGPGVQR
                     GGTGVVRDVRVLAERAELATRMENTVVAPYGVAGGGAGRTGRITLNPGTPEERVLPAL
                     GDGIILKRGDLLRFETCGGGGWGDPLAREPERVRQDVARGFITPRGALEDYGVVVDAA
                     TLEVDKTATQEERGRRARALPLIDRGPGFAQAEERWRGFRSAGR"
     gene            complement(3394..5472)
                     /locus_tag="VFX87_03595"
     CDS             complement(3394..5472)
                     /locus_tag="VFX87_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4994022.1"
                     /translation="MTKTCTLAIDVGGTFTDITFTDAATGALWVAKTPSTPGDLSTGF
                     ITAVRKVLALAGRAPVDVLRVFHGTTTATNAILEGKTPPTALVTTAGFKYVLEIGRHD
                     IPRHGNLYGWSKPTRPITPERVFEVDERLDVDGSVLRPLDEPAARALARQLAQMGVPA
                     VAVVFLHAYANPAHEQRMQAILAEEYPGVLASLSSEVLPQFREFERTMATALNAAVMP
                     PVSRYVSVLRQALDGLGLQAPLLIMKSDGGVTSAATCVRQPVQTVLSGPAAGVVGAVS
                     VGRSAGFGDIISIDVGGTSADICLVRDGRPEVTKDGMIGPFPLKLPIVDIHTIGAGGG
                     SIAAVTGAGRLTVGPRSAGAEPGPVCYRRGGSEPTVTDAHLVLGRIPAALLGGELPLD
                     VPAAREALAARIGRPLGLGAEEAAAGVVEIVDNTMARAIRTVSVGRGHDPRRFALVAF
                     GGAGPLHACRLAELLDIPTVIIPPRPGVLSTWGLLDTDVRSTFVRTAGTGARRLAEAG
                     SRVAELEGAWKDLERQAQAWLEGEQVPDERQRFERGADLRYEHQSFELTCPLGEGPLT
                     AARLEQLVATFHAEHRRLYSYDLPKAPVELVNLRLTAVGTLPHAGRSLPVTGGGTLAA
                     AITGHRPVYFRGTGFVSTPCYTRGGLAPGMIFDGPAIIEQDDATPLVAPGFRARVDDA
                     HNILLERSHA"
     gene            5590..6660
                     /locus_tag="VFX87_03600"
     CDS             5590..6660
                     /locus_tag="VFX87_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX4994023.1"
                     /translation="MEFKDYYKTLGVERTADDKAIKTAYRRLARKHHPDVNKGKADRF
                     KEISEAYTVLGDPEKRKRYDTLGPDWERFAQAGAGAGAGRSPFEGRPGGRPGGQNIRF
                     TQEGDAGGFSDFFRTIFSDLGSFRRGQAGPGATEYEFSDLGDLGGGFGPVSRGSDVEA
                     AIELTLEEAFHGAKKTISLELDEPCPECGGSGNVNRRPCPRCRGGGWTKSTRNLEVKI
                     PAGVDTGSRVRMASEGVGGGSGDRASRGDLYLRVTVAPHPRFERKGDDLHADLPLPVT
                     VAALGGEVSVPTLKGQVSMKIPPETSSGKTFRLPGYGMPRLKGGGAGDQFVRAQITMP
                     AGLGPREKELFQELKKLRPETN"
     gene            6662..>8008
                     /locus_tag="VFX87_03605"
     CDS             6662..>8008
                     /locus_tag="VFX87_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX4994024.1"
                     /translation="MRLDKLTVKAQEAIQAAQSLADQGSHQAVEPEHLLQALLQQQEG
                     VVGPLLAKLGARPEALARQVQTEIEKLPAVRGGAGRQYASPRFEGVINRAWEEAERLK
                     DEYVSTEHLLIAIAQEKGGATGRLLAAAGVSAEAIYRSLVDVRGTQRVTDPNPEDKYQ
                     ALQRYSKDLTDLARKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVAGDVPEGLRNKRLVAVDIGAMVAGSKYRGEFEDRLKAVLREITESNGEIIC
                     FIDELHTLVGAGAAEGAVDAANMLKPALARGELRCVGATTLDEYRKHIEKDPALERRF
                     QPVLVREPSVEDTIAILRGLKEKYEVHHKVKIKDSALVAAAVLSHRYIADRFLPDKAI
                     DLIDEAASRLRIEIDSLPVEIDEIERRMMQLQIERVSVGRETDALSLERLAKLDAEL"
BASE COUNT         1125 a         2818 c         2956 g         1109 t
ORIGIN      
        1 accgactacg tgacgcgggc ggcccggggc gacctgggcg tgtcgctgcg gagcaacctg
       61 ccggtggccg cgctgatccg cgagcggctg cccgccaccg tcgagctcac ggtggccgcg
      121 ctccttctcg cggtggtcgt cgcgattccg gccggcatcg tctccgcggt caagcgcggc
      181 tcggcgctcg accgcctcgc gatggtgggc gcggtggccg gccaggcggt gccgatcttc
      241 tggctcgcgc tgctcctgat cgccttcttc ggcgtctacc tccgctggct gccggtcttc
      301 ggccggggct cgccggcgca tctggtgctt cccgcggtga gcctctccac cgtgatcctg
      361 ggtcggctgg cccggttggt gcgctcgagc atgctcgagg tcctcgggca ggactacgtg
      421 cgcaccgcgc gcgccaaggg cgtcggcgag gtgcgggtgc tcgccgggca cgcgctgaag
      481 aacgcggcca tcccgatcgt caccctgctc ggtctgcaat tcgcccagct cctgggcggc
      541 gccgtggtga ccgagaccat cttcgcgtgg ccgggcatcg gtcgcctggt ggtggaggcg
      601 atcttcaacc gcgacttccc ggtggtgcag ggcgtggtgc tggtggtctc gctgatcttc
      661 gtggcggtga acctgctggt ggacctggcc tacgccgcgc tcgacccgcg catccgcacc
      721 gaggccgcct gatgcgccgc gactgggcct tctggctctc cgcggcggcg ctcgcgatca
      781 tcgtgctggc cgcggtcttc gcggagtggc tggcccccta cgggcccaac gagcaggaca
      841 tcctcacgcg gctgcagccc cccgacctat ccgcccacgt gctcggcacc gacgaggtcg
      901 gccgcgacat cctgagccgc ctgatccacg gcgcgcgcat ctcgctgctg gtgggggtga
      961 tcgcggtcgg cttctcctgc ccgctcggcg tgcgggtggg cctggtggcg ggctacgcgg
     1021 ggcggcgcgt cgactcggtg ctgatgcacg tcaccgacat ccagctcgcg atccccacca
     1081 tcctgctcgc catcgcggtg gtggcggtgc tcggccccgg gctgcccaac gtgatcctca
     1141 cgctgagcgt gaccggctgg accctctatg cgcgcctggt gcggggcgag accctgggcg
     1201 tcaagacccg ggacttcgtg caggccgccc gcgccaacgg cgcgggcgac gcgcgcatca
     1261 tgacccgcca cgtgctgccc aacgtgctct ccccggtgat cgtggtcgcg gtcttcgcgg
     1321 tggccaacat gatcgtgctc gaggccacgc tctcgttcct gggcctgggc gtggagccga
     1381 gcgtggtgac ctggggtcgg atgctcaacg ggggccggct ctacctcaac accgcgtggt
     1441 ggctcaccgc cttccccggc ctcgcgatct tcgtcaccgt gctggcggtg aacctgctgg
     1501 gcgatcacct gcgcgactgg ctcgacccgc gcctgcgagg ctcgctcggg tcggcgcagg
     1561 cctgagcgtc agcggcccgc gctccggaac ccgcgccagc gctcttccgc ctgcgcgaag
     1621 ccggggcccc gatcgatgag cggtagagcg cgggcgcggc gcccgcgctc ctcctgggtg
     1681 gctgtcttgt ccacctcgag ggtggccgcg tccaccacca cgccgtagtc ctcgagcgcg
     1741 ccgcgcggcg tgatgaagcc gcgcgccacg tcctggcgca cgcgctccgg ctcgcgggcg
     1801 agcggatcgc cccagccgcc accaccgcag gtctcgaagc gcagcaggtc gccgcgcttc
     1861 aggatgatcc cgtcgccgag cgcgggcagc acgcgctcct ccggcgtgcc cgggttgagc
     1921 gtgatgcgcc cggtccggcc cgcgccgccg cccgccaccc cgtagggcgc gaccaccgtg
     1981 ttctccatcc gcgtggccag ctcggcgcgc tcggccagca cccgcacgtc gcgcaccacg
     2041 ccggtgccgc cgcgctggac gccgggcccg cccgagtccg gccgcaccgc gtagcgctcc
     2101 acccggagcg ggaagtcgcg ctccacgtac tccaccgggt agttctcctg ggcgatgtaa
     2161 tagatgacgt ccaccccgtc cgcgaacggc cgcgccccga gccccacccc gagcccttcg
     2221 atgcagagga tcgggcgctg acgggccgcg tcccaggacc ggaagtagta gaggacgtag
     2281 accggcgagc cggcgggcac gcggccgccg ttggcctggg ccagcgtgcc gaagatgcag
     2341 gacatcaccc ggaatcgcgt gttggcgcgc atgccgagcg gcgccgggaa gcgcggcttg
     2401 aggatgctgc cctcgcgggc gatccactcg tcgagattgt ggagcagccc ctcgttgact
     2461 tcgagatcgg cgtcgagcga ctgcaggtac cggccgaagg cgatgcggag cagcccgggg
     2521 ttggtcgtga agttgatcgg gccgcgcgcc tggtcgtcgg agttcgagcc gtcgaggcgc
     2581 acgcggtcac ccacgcgcgt gagcgtgagg tcgatgcgat agggccgggc ctcggtcccg
     2641 ccgtcgctgt ccaggaagtc gtggaagcgc cactcccccg gcggcaccag caggaggaat
     2701 agctcgcgcg cccgcaccgc ggtctgggcg atgcactcgt cgaaggcggc cagcagcgtg
     2761 tccaccccgt agcgggcgca gagctcgccc agccgggtct cggccaggtg gcaggaggcc
     2821 atcatggcgc gggtgtcgcc ctccaccagg tcgggcaggc gtgagttgcg gaggaagatc
     2881 cggtacgcct cctcgttgag ctggccttcg cgaacgattc gcacgggggg cacgagcacg
     2941 ccctcgtgat agatctcggt ggcgtgcggc gagatcgagc ccgcccgtag cccgccgatg
     3001 tcctggtagt gaccgaacgt ggtggcgaag gccaccggcc ttccctcgtg gaagaccggc
     3061 accgtgaaca ccatgtcctg gtggtgctgc acggcgccgt ccgagatgta cggatcgttg
     3121 aaccagtaga cgtcgcccgg ccgcatggtc tcgagcgggt agagtgtcaa gatcggctgc
     3181 agcatgccgg gcccgctgcc cgagatgtgg caggccacga tgcggccccg cgtgtcgaag
     3241 atgccgacga agtaatcctt cttctccttg atgaagggcg acatcgcgca gcgctccatc
     3301 aggagctcca tgtcgcggat cgcggaggcg aggctgccgc ggatgatctc gagcgtgatc
     3361 ggatcgaccg gcgggatctc ggacgtgggg ggctcaggca tgggagcgct ccaggagaat
     3421 attgtgcgcg tcgtcgacgc gggcgcggaa gcccggcgcc accagcgggg tggcgtcgtc
     3481 ctgctcgatg atggcggggc cgtcgaagat catgcccggc gcgaggccgc cccgcgtgta
     3541 gcagggcgtg gagacgaagc ccgtgccgcg gaagtagacc gggcggtggc cggtgatcgc
     3601 ggcggccagc gtgcccccgc ctgtcacggg caggcttcgc ccggcatgcg gcagcgtgcc
     3661 caccgcggtg agccgcaggt tcacaagctc cacgggcgcc ttcggcaggt cgtagctgta
     3721 gagccgccgg tgctcggcgt ggaaggtcgc cacgagctgc tcgagccggg ccgcggtcag
     3781 cgggccctcg cccagcgggc aggtcagctc gaagctctgg tgctcgtagc gcaggtcggc
     3841 gccgcgctcg aagcgctggc gctcgtccgg cacctgctcg ccctcgagcc aggcctgggc
     3901 ctggcgctcc aggtccttcc acgcgccctc gagctccgcc accctgctcc ccgcctcggc
     3961 cagccggcgc gccccggtgc ccgcggtgcg cacgaaggtc gagcggacgt cggtgtcgag
     4021 cagaccccag gtcgagagta cgcccggccg cggcgggatg atcacggtcg ggatgtcgag
     4081 cagctcggcc aggcggcagg cgtgaagcgg gccggcgccg ccgaaggcca ccagggcaaa
     4141 gcgccgcggg tcgtggccgc gccccaccga cacggtccgg atggcgcgtg ccatggtgtt
     4201 gtccacgatc tcgacgacgc cggcggccgc ctcctccgcc ccgagaccga gcgggcggcc
     4261 gatgcgggcc gcgagggcct cgcgcgccgc cggcacgtcg agcggcagct cgccgcccag
     4321 cagggcggcc gggatgcggc ccagcaccag gtgcgcgtcg gtgacggtgg gctcgctccc
     4381 gccgcggcgg tagcacacgg gcccgggctc ggcgcccgcg ctgcgcggcc ccaccgtgag
     4441 gcggcccgcg ccggtcaccg cggcgatgct cccgccgccc gcgccgatcg tgtggatgtc
     4501 cacgatgggc agcttgagcg ggaacggccc gatcatcccg tccttggtga cctcgggccg
     4561 cccgtcgcgc accaggcaga tgtcggcgct ggtgccgccc acgtcgatcg agatgatgtc
     4621 gccgaagccg gcggagcgcc cgaccgacac cgcgccgacg acgccggcgg cggggccgga
     4681 gagcacggtc tgcaccggct ggcgcacgca ggtggccgcg ctggtgacgc cgccgtccga
     4741 tttcatgatg agcagcggcg cctgcaggcc gaggccgtcc agcgcctggc gcagcacgct
     4801 cacgtagcgg ctcaccggcg gcatcaccgc cgcgttgagc gccgtcgcca tcgtgcgctc
     4861 gaactcgcgg aactggggca ggacctcgga ggagagcgag gcgagcacgc ccgggtactc
     4921 ctcggccagg atggcctgca tccgctgctc gtgcgcggga ttggcgtacg cgtgcaggaa
     4981 caccaccgcg accgcgggga cgcccatctg ggcgagctgg cgggccaggg cgcgcgcggc
     5041 gggctcgtcg agcgggcgca ggaccgagcc gtccacgtcg agccgctcgt ccacctcgaa
     5101 cacacgctct ggcgtgatcg gccgcgtggg cttggaccag ccgtagaggt tgccgtggcg
     5161 cgggatgtcg tggcggccga tctcgagcac gtacttgaag ccggcggtgg tcaccagcgc
     5221 ggtgggcggc gtcttgccct cgagaatcgc gttggtggcg gtggtggtgc cgtggaagac
     5281 gcgcagcacg tcgaccggcg cccgcccggc cagggccagc accttgcgca cggcggtgat
     5341 gaagccggtg gagaggtcgc ccggcgtgga gggcgtcttc gcgacccaga gtgcgccggt
     5401 ggcggcgtcg gtgaacgtga tgtcggtgaa ggtcccgcct acgtcgatgg cgagcgtgca
     5461 ggtcttggtc acgggcgcgc ccattgtagt cccgcggacg atggggggaa agagagaccc
     5521 gcgcccagcc aaccgggcga ccgtctgcta cacttccagt aggcgagggc catctcgtca
     5581 cctaacccta tggaattcaa ggactactac aagacgctcg gggtcgagcg gaccgccgac
     5641 gacaaggcga tcaagaccgc ctaccgccgg ctcgcccgca agcatcaccc cgacgtgaac
     5701 aaggggaagg ccgaccgctt caaggagatc agcgaggcct acacggtcct cggggacccc
     5761 gagaagcgga agcgctacga caccctcggg cccgactggg agcgcttcgc ccaggccggc
     5821 gccggcgcgg gagccgggcg ctcgcccttc gagggccgcc ccggaggccg ccccggcggt
     5881 cagaatatcc gcttcacgca ggagggcgac gcgggcggct tctcggattt cttccgcacg
     5941 atcttctcgg acctgggcag cttccgccgc ggccaggccg gccccggcgc aaccgagtac
     6001 gagttctcgg acctgggcga cctgggcggc gggttcggcc cggtgagccg cggcagcgac
     6061 gtcgaggccg ccatcgagct cacgctggag gaggccttcc acggggcgaa gaagacgatc
     6121 tcgctcgagc tcgacgagcc gtgcccggaa tgcggcggct ccggcaacgt caaccggcgc
     6181 ccctgcccgc ggtgccgggg cggcggctgg accaagagca cgcggaacct ggaggtgaag
     6241 atcccggcgg gcgtggacac cggctcgcgc gtgcgcatgg cgtccgaggg cgtcggcggc
     6301 ggctcgggcg accgcgcgtc ccgcggcgat ctctacctgc gcgtgacggt ggcgccgcac
     6361 ccgcgcttcg agcgcaaggg cgacgatctc cacgcggacc tgccgctgcc ggtgacggtg
     6421 gccgcgctcg gcggcgaggt ctcggtgccc acgctcaagg ggcaggtgtc catgaagatc
     6481 ccgccggaga cgtcgagcgg caagacgttc cggcttcccg ggtacggcat gccgcggctc
     6541 aagggcggcg gagccggcga ccagttcgtg cgggcgcaga tcacgatgcc agccgggctc
     6601 ggcccgagag agaaagagct attccaggag ctgaagaagc tcagaccgga gaccaactga
     6661 gatgaggctg gacaagctga ccgtcaaggc ccaggaagcc atccaggccg cccagtcgct
     6721 ggccgaccag ggcagccatc aggccgtcga gcccgagcac ctgctgcagg cactgctgca
     6781 gcagcaggag ggcgtggtgg gaccgctgct cgccaagctg ggcgcgcggc ccgaggcgct
     6841 cgcgcgccag gtacagaccg agatcgagaa gctcccggcg gtgcgcggcg gcgccgggcg
     6901 gcagtacgcc tccccgcgct tcgagggcgt gatcaatcgc gcgtgggaag aggcggagcg
     6961 gctcaaggac gagtacgtct cgaccgagca tctgctgatc gccatcgccc aggagaaggg
     7021 cggcgccacc gggcgcctgc tcgccgcggc gggcgtgagc gcggaggcga tctaccgctc
     7081 gctggtggac gtgcgcggca cgcagcgcgt gaccgatccg aatcccgagg acaagtacca
     7141 ggcgctccag cgctactcca aggatctgac cgacctggcc cgcaagggca agctcgaccc
     7201 ggtcatcggg cgtgacgagg agatccgccg ggtgatccag gtgctctcgc gccggaccaa
     7261 gaacaacccg gtgctgatcg gcgagcccgg cgtgggcaag accgccatcg tcgaaggcct
     7321 cgcccagcgc atcgtcgccg gcgacgtgcc ggagggtctg cggaacaagc gcctcgtggc
     7381 cgtcgacatc ggggccatgg tcgcgggctc caagtaccgc ggcgagttcg aggaccgcct
     7441 gaaggcggtg ctgcgcgaga tcaccgagtc caacggcgag atcatctgct tcatcgacga
     7501 gctgcacact ctggtgggcg cgggcgcggc cgagggcgcg gtggacgcgg ccaacatgct
     7561 gaagccggcg ctggcccgcg gggagctgcg ctgcgtgggc gccaccacgc tcgacgagta
     7621 ccgcaagcat atcgagaagg accccgcgct ggagcgccgc ttccagccgg tgctggtgcg
     7681 cgagccctcg gtggaggaca ccatcgcgat cctgcggggc ctcaaggaga agtacgaggt
     7741 ccaccacaag gtgaagatca aggactcggc cctggtggcg gcggcggtgc tctcgcatcg
     7801 gtacatcgcc gaccgcttcc tccccgacaa ggccatcgac ctgatcgacg aggcggcgtc
     7861 gcggcttcgc atcgagatcg attccctgcc cgtcgagatc gacgagatcg agcggcgcat
     7921 gatgcagctc cagatcgagc gcgtgtcggt ggggcgcgag accgatgcgc tctcgctcga
     7981 gcgcctcgcc aagctcgacg ccgagctg
//
LOCUS       DASUFA010000096         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_938989, whole genome shotgun sequence.
ACCESSION   DASUFA010000096 DASUFA010000000
VERSION     DASUFA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938989"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..764)
                     /locus_tag="VFX87_03610"
     CDS             complement(66..764)
                     /locus_tag="VFX87_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994025.1"
                     /translation="MKESLELEAVQAGYGLSRVLHGVTLAARAGEVVSLLGRNGAGKS
                     TTLKAIVGLVEVTGGAIHFAGHEITRRPTHEISRLGVGYVPEDRRIFADLTVEENLTV
                     GAKGGGVWSEARVYQFFPKLLELAGRRAGSLSGGEQQMLTVARTLMGNPRVLLLDEPS
                     EGLAPVIVRALGDQIAALKREGLTILLSEQNLKFAARLADRAYIIEKGEIRFDGPMTQ
                     LMGDEALRRKYLTV"
     gene            complement(817..1581)
                     /locus_tag="VFX87_03615"
     CDS             complement(817..1581)
                     /locus_tag="VFX87_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994026.1"
                     /translation="MSAVLGGVPVLETRGLSKAFGGVRAVRDVDLAMPKGEIRALIGP
                     NGAGKTTFFNMLTGQLKADAGLVLFKGERLSGLPPYAVWRRGVSRTFQITATFATLTA
                     LENVQVARLSHLGKTYALLERAQHLQAEEARALLEQVGLADQAGRVAAVLAYGDLKKL
                     ELAVALANDPEVLLLDEPTAGMAPAERGALMALTQRIARARGLTVLFTEHDMDVVFAI
                     ADRIMVLHQGRVIADGAPAAVRADAQVQRVYLGEDE"
     gene            complement(1578..3404)
                     /locus_tag="VFX87_03620"
     CDS             complement(1578..3404)
                     /locus_tag="VFX87_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994027.1"
                     /translation="MSLLFVQLLSGLANAMFLFLIASGLSLIFGVTRIVNFAHGSFYM
                     LAAYLTYSLAAALPGGPVAFYAAVLLAAVLVAALGGLVEVLLLRRVYKAPELYQLLLT
                     FALVLVTADAVRYLWGADNKTGPNAPGLAGSVPIAGQLFPSYDLAVIAFGPLVALGLW
                     LLFHRTRWGILIRAATQDREMVAALGVDQSRLFTSVFVLGSFLAGLGGALQVPRLALT
                     TVMDTSIIVEAFVVVVIGGMGSVWGALLASLLIGVLNAYGVLLLPKIAIVLIFVVMAV
                     VLVVRPWGLLGRPEIQLRPPGSGVVAEAQPRRLGPIWLGAGVMALAALPLVLPTFWIS
                     IAAEIFAFALFAASLHLLMSVGGMVSFGHAAYFGLGAYGAAVLLQLAGLPMPAAFVLA
                     PVVAGLAACVFGYFCVRLTSIYFAMLTLAFAQIVYAVVHQWDDITGGDNGMLSVWPAR
                     WLGTPSRYYYWALAATLVGLFLLRLVTASPFGLTLRAVRDHARRAEAVGVNIRALQWT
                     AFVVAGFVAGLGGAIYAFLKGSVFPVYTESPMSVQPLVMVLLGGVGSPAGPLIGAAVY
                     KLLDTVITRYTDYWQIVLGAILLVLVLAFPRGIAGMLDGRRS"
     gene            complement(3478..4692)
                     /locus_tag="VFX87_03625"
     CDS             complement(3478..4692)
                     /locus_tag="VFX87_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994028.1"
                     /translation="MRALRLLSLMAAALLPTLWAGAATAQAPIKLGDINTYSGIGAPF
                     TGPYRQGVEMAVEEINAKGGVLGRKVEVLFRDDKAQPAEAVKHAQELVESDKVAMITG
                     SFLSNVGLAVSDWAKQNKTMFLASEALTEALTWSKGHDYAVRLRPNTYEQGRMLADKA
                     GKMKYVKWATIGPNYEYGKRAWETFRDRLKELRPDVQMVGEHWPTLGKIEPGPLVTAI
                     LNQNPDALYVSLFGSDWLAFVREAAKRGLFQKMFVVGILLGEPEYIDPLKLEAPEGML
                     VTGYPWYDVKTAGHPEWVARYIKRGDKPPVLGSLIGYITYLSVAEAIKKAGSTETPKL
                     VAAFKGLKVETPMGPIMFRAADGQSTMGAWVGTTKLDPQRGVGIMVNHEYVPGEKVLP
                     TEEEAKKLRPAN"
BASE COUNT          784 a         1655 c         1710 g          657 t
ORIGIN      
        1 cggcctcctt atgtccctct cccctcaggg gagagggcag ggtgaggggt ggttcctcgt
       61 ggacctcaca ccgtcagata ctttcgccgc agcgcctcgt cgcccatgag ctgggtcatg
      121 ggcccgtcga agcggatctc ccccttctcg atgatgtacg cgcggtcggc gagccgcgcc
      181 gcgaacttga gattctgctc ggagagcagg atcgtcaagc cctcccgctt gagggcggcg
      241 atctggtcgc ccagggcccg tacgatcacg ggggcgaggc cttccgacgg ctcgtccagc
      301 agcagcacgc gggggttgcc catgagcgtg cgcgccaccg tcagcatctg ctgctcgccg
      361 cccgagaggc tcccggcccg gcggcccgcc agctccagga gcttgggaaa gaactggtag
      421 acccgcgcct cgctccagac gccgcctccc ttggcgccca ccgtcaggtt ctcctcgacg
      481 gtgagatcgg cgaagatgcg ccggtcctcc ggcacgtacc cgaccccgag gcggctgatc
      541 tcgtgggtcg gccgccgggt gatctcgtgc ccggcgaagt ggatggcgcc gccggtcacc
      601 tccaccaggc ccacgatggc cttgagcgtg gtggacttgc ccgcgccgtt gcgccccagc
      661 agcgagacca cctcacccgc ccgcgcggcc agcgtcacgc cgtggagcac gcgcgagagc
      721 ccgtagccgg cctggaccgc ttccagctcg agagactctt tcaaggccct tgttgaccgt
      781 ggtcgagatg gtcggggcga ccggagccgg gcagtctcac tcgtcttcac cgaggtagac
      841 gcgctggacc tgggcgtcgg cgcggacggc ggcgggagcg ccgtcggcga tgacccggcc
      901 ctggtggagc accatgatcc ggtcggcgat ggcgaagacc acgtccatgt cgtgctcggt
      961 gaagagcacg gtgagcccgc gggcgcgggc gatgcgctgg gtgagcgcca tgagcgcccc
     1021 gcgctcggcg ggcgccatgc ccgcggtggg ctcgtcgagc agcagcacct cggggtcgtt
     1081 ggcgagggcc accgccagct cgagcttctt gagatcgccg taggcgagca ccgccgcgac
     1141 ccgcccggcc tggtcggcca gacccacctg ctccagcagg gcgcgcgcct cctcggcctg
     1201 caggtgctgg gcccgctcca gcagcgcgta ggtcttgccg aggtgcgaga gccgcgccac
     1261 ctgcacgttc tcgagcgcgg tcagcgtggc gaacgtggcg gtgatctgga aggtccggct
     1321 cacgccgcgc cgccagaccg cgtagggcgg gagcccgctg agccgctcgc ccttgaagag
     1381 gacgaggccc gcgtcggcct tgagctggcc ggtgagcatg ttgaagaagg tggtcttgcc
     1441 cgcgccgttg ggcccgatca gcgcgcggat ctcccccttc ggcatcgcga ggtccacgtc
     1501 ccggaccgcg cgcacgcctc cgaaggcctt ggacagcccg cgcgtctcga gcaccggcac
     1561 gcccccgagg accgcgctca cgagcggcgc ccgtcgagca tgcccgcgat accgcgcggg
     1621 aacgccagga ccagcacgag caggatggcg cccagcacga tctgccagta gtccgtgtag
     1681 cgcgtgatca cggtatcgag cagcttgtag accgcggcgc cgatgagcgg cccggccggt
     1741 gagcccacac cgcccagcag caccatcacc agcggctgca ccgacatggg cgactcggtg
     1801 tagaccggga acacgctgcc cttgagaaag gcatagatag cgccgccgag gcccgccacg
     1861 aagccggcca ccacgaacgc ggtccactgc agcgcgcgga tgttcacgcc gaccgcctcc
     1921 gcgcggcgcg cgtggtcgcg caccgcccgc agcgtgaggc cgaacggcga ggcggtgacc
     1981 agccgcagga ggaacaggcc cacgagggtg gcggcgaggg cccagtagta gtagcggctc
     2041 ggcgtgccca gccagcgggc gggccagacg ctcagcatgc cgttgtcgcc ccccgtgatg
     2101 tcgtcccact ggtgcacgac cgcatagacg atctgggcga aggcgagcgt cagcatcgcg
     2161 aagtagatgc tggtgagccg cacgcagaag tagccgaaga cgcaggcggc cagcccggcc
     2221 accaccggcg cgaggacgaa cgcggcgggc atgggcaggc cggccagctg gaggagcacc
     2281 gcggcgccgt aggcacccag gccgaagtag gccgcgtggc cgaacgacac catgccgccc
     2341 accgacatca gcagatgcag gctggccgcg aagagcgcga aggcgaagat ctcggccgcg
     2401 atggagatcc agaaggtggg cagcaccagg ggcagcgcgg ccagcgccat caccccggct
     2461 ccgagccaga tcggcccgag ccgccgcggc tgcgcctcgg ccaccacacc gctgccgggc
     2521 gggcggagct ggatctcggg ccggcccagc aggccccacg gccgcaccac cagcaccacc
     2581 gccatcacca cgaagatgag cacgatggcg atcttcggca gcaggagcac gccgtacgcg
     2641 ttgagcacgc cgatgagcag cgaggcgagc agcgcgcccc agaccgagcc catgcccccg
     2701 atcaccacca ccacgaacgc ctccacgatg atggacgtgt ccatcacggt ggtgagcgcc
     2761 agccgcggca cctggagcgc gccgcccagg ccggccagga acgagcccag cacgaacacg
     2821 gaggtgaaaa ggcgcgactg gtccaccccg agcgcggcga ccatctcgcg gtcctgggtg
     2881 gcggcacgga tcaggatgcc ccagcgcgtg cggtggaaga gcagccagag cccgagcgcc
     2941 acgagcgggc caaacgcgat gaccgcgagg tcgtaggacg gaaagagctg gcccgcgatc
     3001 ggcaccgagc cggccaggcc cggcgcgttc ggaccggtct tgttgtcggc gccccagaga
     3061 taccggaccg cgtcggcggt caccagcacg agcgcgaagg tgagcaggag ctggtagagc
     3121 tcgggcgcct tgtagacgcg gcggagcagc aggacctcga ccagaccgcc cagcgccgcc
     3181 accagaaccg ccgcgagcag cacggccgcg tagaaggcga ccggcccccc gggcagcgcc
     3241 gcggccagcg agtaggtcag gtaggcggcg agcatgtaga aggagccgtg ggcgaagttg
     3301 acgatgcggg tgacgccgaa gatcagcgag agaccggagg cgatgaggaa gaggaacatc
     3361 gcgttggcga gcccgctcag gagctggacg aagaggaggg acatgaggag gtgcgggccc
     3421 cctcaccctg ccctctcccc cgaggcgggg gagagggaga tcgagtgagg ggtggtctta
     3481 attggccggg cggagcttct tggcttcttc ttcggtcggc agcaccttct cgcccggcac
     3541 gtactcgtga ttcaccatga tgccgacgcc gcgctgggga tcgagcttgg tcgtccccac
     3601 ccacgcgccc atggtggact gcccgtccgc ggcgcggaac atgatcggtc ccatcggggt
     3661 ctcgaccttc aggcccttga aggcggccac cagcttgggc gtctcggtgc tgcccgcctt
     3721 cttgatggcc tcggccaccg acaggtaggt gatgtagccg atgagcgagc cgaggaccgg
     3781 gggcttgtcc ccgcgcttga tgtagcgcgc cacccactcc gggtgcccgg cggtcttcac
     3841 gtcgtaccag gggtacccgg tgaccagcat gccctcgggc gcctcgagct tgagcgggtc
     3901 gatgtactcg ggctcgccga gcaggatgcc gaccacgaac atcttctgga agagcccgcg
     3961 cttggcggcc tcgcgcacga aggcgagcca gtcgctaccg aagagcgaga cgtagagcgc
     4021 gtccgggttc tggttcagga tcgcggtcac gagcgggccc ggctcgatct tgccgagcgt
     4081 cggccagtgc tcgcccacca tctgcacgtc gggccggagc tccttgagcc ggtcccggaa
     4141 ggtctcccac gcccgcttgc cgtactcgta gttggggccg atggtggccc acttcacgta
     4201 cttcatcttg ccggccttgt ccgccagcat gcgcccctgc tcgtaggtat tggggcgcag
     4261 gcgcaccgcg tagtcgtgcc ccttggacca ggtgagggcc tcggtcagcg cctcggaggc
     4321 caggaacatg gtcttgttct gcttggccca gtccgagacc gcgagcccca cgttggagag
     4381 gaagctcccg gtgatcatgg cgaccttgtc ggactcgacc agctcctggg cgtgcttgac
     4441 cgcctcggcg ggctgggcct tgtcgtcgcg gaacaggacc tcgaccttgc ggccgagaac
     4501 gcctcccttg gcgttgatct cctcgacggc catctcgacg ccctggcggt agggcccggt
     4561 gaagggcgcg ccgataccgc tgtaggtgtt gatgtcgccg agcttgatcg gcgcctgggc
     4621 cgtggcggcg cctgcccaca gcgtgggcag gagagcggcc gccatgaggg acaggagacg
     4681 gagtgcacgc atgcggaacc tcctcgtggt tcgtgttgcg ctgagtaaag caaatgcgcg
     4741 gagagagtca agacgattct ccgagcttga cagggagcat agccgagtgg tttcctctgg
     4801 ggcaag
//
LOCUS       DASUFA010000097         9376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1615269, whole genome shotgun sequence.
ACCESSION   DASUFA010000097 DASUFA010000000
VERSION     DASUFA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615269"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="cobU"
                     /locus_tag="VFX87_03630"
     CDS             complement(<1..564)
                     /gene="cobU"
                     /locus_tag="VFX87_03630"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX4994029.1"
                     /translation="MTAVHSSHLVLGGARSGKSRYAVEQVAQSGSRVAFLATARALDG
                     DMAARIARHRAERPARWPTLEEPHDLVGACRRAATAHDLVLVDCVTLWVANLMDRGDD
                     DTAVLAAAEDLAKLQRERVVSLLIVSNEVGEGVHPSTALGRRFRDLLGSVNQRLAAAA
                     DRVTLMVAGLPVTVKDTPPPPARRDPTH"
     regulatory      complement(609..740)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(830..1195)
                     /locus_tag="VFX87_03635"
     CDS             complement(830..1195)
                     /locus_tag="VFX87_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX4994030.1"
                     /translation="MSDVRIDKWLWAARFFKSRTLAAAACNGGKVDVNGDAAKPSKTV
                     RPGDLLRVTLPRIRRVVRVTALAERRGAASEAAVLYEDLTPPPPPREARPAPPAYRPP
                     GAGRPTKRERRRIDRLADW"
     gene            1243..1938
                     /locus_tag="VFX87_03640"
     CDS             1243..1938
                     /locus_tag="VFX87_03640"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX4994031.1"
                     /translation="MFRAAIGLVTGMTLLALTLVASGPLSAADPAAVLVEDWSKQPEG
                     KTGIPDGWKGQSWGSPKYDFRIATQSGRKVLHLKSANDSSTISKEIKVDVKKHPILQW
                     SWQAVTLPKGGDARTSATDDQAAQLYVTFPRFPSQVRSRIISYLWDTTAPVGAVFKSE
                     KTGLVTYVVVRSGPADLGKWQTESRNVLEDYKKIYGEAPGEEAGAISISIDSNDTRSS
                     AESYFGELLFRKP"
     gene            1959..2345
                     /locus_tag="VFX87_03645"
     CDS             1959..2345
                     /locus_tag="VFX87_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX4994032.1"
                     /translation="MASYGAMLLRLILGIVYIMHAYLALVVFGPSGMIAYQAKAGVPF
                     PDLATWYLILAHGLGGIFLVLGIYTRWAALANVPVMLGAVVFVHLKNGFWAHQTPNAG
                     YEYVLVLLVATLASAMIGGGALSLKR"
     gene            complement(2355..3092)
                     /locus_tag="VFX87_03650"
     CDS             complement(2355..3092)
                     /locus_tag="VFX87_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994033.1"
                     /translation="MPLLLFVAALLLAACQQPSARQVPATSAVSPAEQMLAEGGALMA
                     QGNYVTAVEKLRQAADLEPGSVPVRFALGTAYSFLEKRPEAIAQFRWVMSNARIESTE
                     RAEARRWLVRVGALVEPPPAPGKLDVASSDAVAKKVDPAAQGSISGQTQWTGVTPAEH
                     QLPVTIAIVGSEDATRELRQRAQIQLGERFEFKDVREGQYRLIGAFADKILWDQSVTV
                     KGGKQTDVALSQATSLVPVNTFPLPAH"
     gene            complement(3096..4580)
                     /locus_tag="VFX87_03655"
     CDS             complement(3096..4580)
                     /locus_tag="VFX87_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994034.1"
                     /translation="MKTWRKMLGNERGVALPLAMIMLVVLTMLTLTFMSLGAVEPQIS
                     RNLSDGARARQLAESGIEWGFNQLAGRDFNDTALLGSGTTTGGLCGSYTCKVLTSSTA
                     LPGLTSATGTFAVTLRNDLSTVNGDQALIGSGNTLDASATLDNNKVVILRATGTFNGA
                     SRTITAVVQRGNLPINGAVSLPGVQGDTYTQAPPCAISVGCYWIDGRDYRDADTWGPA
                     GTPTGVGALKYAMTTATAAIETVAESGFNTLAKREYLHGSNDGNGYTAATDGTAVTGA
                     AGNGRNTIADDPSLSPALIAKFMSNLASNPSTQILDSTMACQYPAPGGVHDKPEGLQM
                     ASTGTPNIVTVTNNCAGAAQINQTVNLGTASAPAMLYVRGEYDPTSLFRGLAVSGSNP
                     ITGYGILVVEDSDLAFFQTGNFRWNGIVLVTGRNNSTAFLGSSNTEIRGALITSETNP
                     LEAAGFNEFFAGTSGTMRILASKENIDRALMALYNMRISAYREY"
     gene            complement(4610..5290)
                     /locus_tag="VFX87_03660"
     CDS             complement(4610..5290)
                     /locus_tag="VFX87_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4994035.1"
                     /translation="MNDQRGFTLAEMLVVCAIVGLVMASLLGLTMQGQRAYWFGTTQV
                     DGQQTVRVALERLAKEVREAGYEPLPGETDPTACPNATNYPLYTAGVPCYKFVPITSP
                     AATSLTLQYNWDGSTCAVPCSPISTAALVNDPMTCAAPAAACRGEQVTYSLSAGNLRR
                     QESVVDSAPVTIASGITALTFTYRDENNNVTASPELIRSVEISMTVQTATRGAYVTMV
                     DRVRLRNR"
     gene            complement(5305..5754)
                     /locus_tag="VFX87_03665"
     CDS             complement(5305..5754)
                     /locus_tag="VFX87_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4994036.1"
                     /translation="MHVLSRLKKSRPGEPGFTLAEILVACAIIAVGLVAVATGFGFGV
                     DGVEAGRQQSTAVFLAEQRIEQAKALAMRQTGLVQLTVANLPASEAYGTIAGAPPSYR
                     RTTTITTNPGGTLGARVDVVVFYRPVTGRGVLTTERSVSQSIFLANR"
     gene            complement(5978..7594)
                     /locus_tag="VFX87_03670"
     CDS             complement(5978..7594)
                     /locus_tag="VFX87_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994037.1"
                     /translation="MTAGALGRRRFLALAAGAASAAAARRAGATERPVRGGVLKVIGL
                     EPPTFDVHATASPQTQLVSSLVRRTLFKVVHGARYGPSDFTRAPDLALSAVASADGRT
                     YTIALRPGVRWESRPPVNGRELVASDVKHSLERALRTSCFAPLLGPVERIDAPDRRTV
                     RVHLADTFAPFVHNLAEPWTAILPPEVEDRPADPKAAESLIGCGPFVLERYEPGVKAV
                     FSRNPTYHARGLPYLDKVEWLFIKDRPTQLALFRAGQVDLLGHDARIPRAEVAAFKKA
                     NPGYPVVSWDGLEIRSLAMRADRPPFNDVRVRRALSLAVDRKTWVAEYLAGDGAEAHG
                     PVPSAMRQWKLAARHLGEGARYLEHDPVRARKLLAEAGFPAGLKIKCTSWPGYGPEYV
                     EDLERLAGHLRRVGVELQIVPEEYGQYIRGSFLGRFEEASWGPSSLFTEVDGYLSSFF
                     RSGQVGNRSHVADAQLDVMLEAQRRFTSSSSRKKIIDEIQRRAAAQVYYVFTPYPRNV
                     SSWAPRVRNYGPKNCFDRGAQLEVVWLDQA"
     gene            complement(7591..8208)
                     /locus_tag="VFX87_03675"
     CDS             complement(7591..8208)
                     /locus_tag="VFX87_03675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4994038.1"
                     /translation="MALSATTVCLVRHGSVVGAETRRFIGHLDVPLSSLGVAQIDALS
                     RRLIGVAFDAIYCSDLERTRRSAEILAAPHALIPVRDPALREFAMGEWDGLTAEQIRV
                     RDARAFEAWMADVGRFQFPGGESLPDLVARAVPAFEALVARHAGGTIAVVAHGGSNRA
                     ILCHALGLGPERLLALGQDYAALSVLERAGGHWSLRLLNHCEPLP"
     gene            complement(8199..8948)
                     /gene="cobS"
                     /locus_tag="VFX87_03680"
     CDS             complement(8199..8948)
                     /gene="cobS"
                     /locus_tag="VFX87_03680"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX4994039.1"
                     /translation="MGSLILALRFLTVVPIPGRESAGPSALGRAAWWFPVVGLLLGSA
                     LAVVASAVDVAFPPLVAAALLVTGWKVATGGIHLDGLADSLDGLAGRDPAGRLAIMRD
                     SRIGVFGAAGLVLLCLLAVTALAALSPSLRLRVLVLAPVIGRVAPLLAGVWLVPATPG
                     KGLGAAFAAGLSRWTGPAHVVAGGALAAWLLGLWGVAVAAAAWSVALLAAGFVARRLG
                     GLTGDVLGALVEVAELGVLLGAAAAAHRGWL"
     gene            complement(8974..>9376)
                     /locus_tag="VFX87_03685"
     CDS             complement(8974..>9376)
                     /locus_tag="VFX87_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003398501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4994040.1"
                     /translation="VFATSQATVPKICVVVRKCYGAGLYAMCGPAFEPDAALALPQGQ
                     VAIMGPEPAVNAVYYNKIMELPEAERPAYVQQKRDEYARDVDIYKLASEMLVDGIVGG
                     SELRDELIKRLEYANSKQLEFPPRRNPVLPV"
BASE COUNT         1386 a         3300 c         3340 g         1350 t
ORIGIN      
        1 atgggtcggg tcgcggcggg ccgggggcgg cggcgtgtcc tttacggtca cgggaagtcc
       61 cgccaccatc agcgtcacgc gatcggcggc ggcggcgagc cgctggttta cggatcccag
      121 gagatcgcga aagcgccggc cgagcgcggt ggagggatgc acgccctcgc ccacttcgtt
      181 ggacacgatg aggagcgaga ccacgcgctc ccgctggagc ttggccaggt cctcggcggc
      241 ggccagcacc gccgtgtcgt cgtcgccgcg atccatcagg ttggcgaccc agagggtgac
      301 gcagtccacg aggaccaggt catgcgcggt ggcggcgcgc cggcaggcgc ccacgagatc
      361 gtgcggctcc tccagggtgg gccagcgggc cgggcgctcg gcccggtggc gggcgatgcg
      421 ggcggccatg tcgccgtcca gggcgcgcgc ggtcgccagg aaggccacac ggctgccgga
      481 ctgcgccacc tgctcgaccg cgtagcggct cttgccgctg cgggcgccgc ccagcacgag
      541 atgcgaggaa tgcacggccg tcacctcggt cctccctcga ggaccggcgc gggtggcagc
      601 gtccgggcag gtctcctggc tctcggatcg ccctactccc cgcaccttcc cgctccggct
      661 gaggccggag cagtggccga ttgcgggttt cgttcccgat tacagtgacg gggtcgcggc
      721 ggattcgcac cgccttccct ggggcccgca cccggcgggc cgcccggaca ccatggacgg
      781 ccagaccgta gcagaggggg cgggtcagcg caaccgcccc gcgtgagcgc taccagtcgg
      841 ccagacgatc gatgcgacgg cgctcgcgct tggtgggacg tccggcgccc ggcggccggt
      901 aggccggcgg cgccgggcgg gcctcccgcg gcggcggggg cggcgtgagg tcctcgtaga
      961 ggaccgccgc ctcgctggcg gctccccgtc gctccgcgag cgcggtcacg cgcacgacgc
     1021 ggcggatccg cggcagggtg acgcgcagca ggtcgccggg ccgtaccgtc ttggacggct
     1081 tcgccgcgtc gccgttgacg tccaccttcc cgccgttgca ggcggcggcc gcgagcgtgc
     1141 ggctcttgaa gaagcgcgcc gcccacagcc acttgtcgat ccgcacgtcg gacatctggt
     1201 ttttaccgta gcacacgggc cccgtgagac actgaatcgg ccatgttccg cgcggcgatc
     1261 gggctcgtga cgggaatgac gctgctggcg ctgacgctcg tcgcgagcgg gccgctctcc
     1321 gcggccgacc cggccgccgt gctcgtcgag gattggtcga agcagcccga aggcaagacc
     1381 ggcatccccg acggctggaa ggggcagagc tggggcagcc ccaagtacga cttccgcatc
     1441 gccacccaga gcggccgcaa ggtcctccac ctcaagagcg ccaacgacag ctcgacgatc
     1501 tccaaggaga tcaaggtaga cgtcaagaag cacccgatct tgcagtggag ctggcaggcg
     1561 gtcacgctcc ccaagggggg cgacgcccgc acgtcggcga ccgacgatca ggcggcgcag
     1621 ctctacgtca ccttcccgcg attcccctcg caggtccgct cgcgcatcat ctcctacctc
     1681 tgggacacca ccgcgcccgt gggggcggtg ttcaagagcg agaagacggg cctcgtgacc
     1741 tacgtggtgg tccgctccgg gccggccgac ctcggcaagt ggcagaccga gagccgcaac
     1801 gtgctcgagg actacaagaa gatctacggg gaggcgccgg gggaagaggc tggggccatc
     1861 tcgatctcca tcgactccaa cgacacgcgc tcctccgccg agtcctattt cggcgaactc
     1921 ctgttccgga agccctgaga gccaggaagg gaggcgcgat ggcatcctac ggcgcaatgc
     1981 tgctccggct gatcctgggt atcgtctaca tcatgcacgc ctacctcgcg ctggtggtct
     2041 tcggcccgtc cggcatgatc gcctaccagg ccaaggcggg ggtccccttc cccgatctcg
     2101 ccacgtggta cctgatcctg gcgcacgggc tgggcggaat cttcctcgtg ctcgggatct
     2161 acacgcgctg ggccgcgctg gcgaatgtcc ccgtcatgct gggcgcggtc gtcttcgtcc
     2221 acctgaagaa cgggttctgg gcccaccaga cgccgaacgc cggctacgag tacgtgctgg
     2281 tgctgctggt ggccaccctg gcgtcggcga tgatcggcgg gggtgccctg agcctgaagc
     2341 ggtagcgccc gcggctagtg cgcgggcagc gggaaggtgt tcacggggac caggctcgtg
     2401 gcctggctca gcgccacgtc cgtctgcttg ccacccttca ccgtgacgct ctggtcccag
     2461 aggatcttgt cggcgaaggc gccgatgagg cggtactggc cttcccgaac gtccttgaac
     2521 tcgaagcgct cgcccagctg gatctgcgcc ctctgtcgca actcgcgcgt cgcgtcctcg
     2581 ctccccacga tcgcgatcgt caccggaagc tggtgctcgg ccggagtgac tcccgtccac
     2641 tgggtctgac cggagatcga gccctgcgcg gccggatcga ccttcttggc cacggcgtcg
     2701 ctggaggcca cgtcgagctt cccgggcgcg ggcggaggct ccaccagggc cccgacgcgc
     2761 acgagccagc ggcgggcctc ggcgcgctcg gtcgattcga tccgagcgtt cgacatcacc
     2821 cagcgaaact gcgcgatggc ctccggccgc ttctcgagga acgagtaggc ggtgccgagg
     2881 gcgaagcgga ccggcacgct gccgggctcg agatcggcgg cctggcggag cttctccacc
     2941 gcggtcacgt agttgccttg agccatgagc gcgcctccct cggcgagcat ctgctccgcc
     3001 ggggagacgg cactcgttgc cgggacctgg cgcgcggacg gctgctggca ggccgcgagg
     3061 agcagcgccg cgacgaagag cagtagcggc accgcctagt actcgcggta tgcggagatg
     3121 cgcatgttgt agagggccat gagcgcccgg tcaatgttct ccttgctggc caggatccgc
     3181 atggtcccgc tggtccccgc gaagaactcg ttgaacccgg ccgcctccaa cgggttggtc
     3241 tcgctggtga tgagggcgcc ccggatctcc gtattgctgg agccgaggaa cgcggtcgag
     3301 ttgttccggc ccgtcaccag cacgatgccg ttccatctga agttgccggt ctggaagaag
     3361 gccagatcgg agtcctcgac cacgagaatg ccgtagcccg tgatgggatt gctgccactc
     3421 acggcgaggc cgcggaagag ggaggtcgga tcgtattcgc ccctcacgta gagcatggcg
     3481 ggcgcgctgg cggtgccgag gttcaccgtc tggttgatct gcgcggcgcc cgcgcagttg
     3541 ttcgtcacgg tcacgatgtt cggcgtgccc gtgctcgcca tctgcagccc ctccggcttg
     3601 tcgtgaaccc cacccggcgc ggggtactgg caggccatgg tgctgtcgag gatctgcgtc
     3661 gagggattcg acgccaggtt gctcatgaac ttcgcgatca gggccggcga gagggagggg
     3721 tcgtcggcga tcgtgttccg gccgttgccg gcggctccgg tgacggcggt gccgtcggtt
     3781 gccgccgtgt acccgttgcc gtcattcgaa ccgtggagat attctctctt ggccagggtg
     3841 ttgaagccgc tctcggcgac ggtctcgatg gccgcggtcg cggtggtcat cgcgtacttc
     3901 agcgcgccga ccccggtggg agtccccgct ggcccccacg tatccgcgtc cctatagtcg
     3961 cgcccgtcga tccagtagca gcccacgctg atcgcacacg gaggcgcctg ggtataggtg
     4021 tcaccctgga cgcccggcag gctcaccgcc ccattgatcg gcaggttgcc gcgctggacc
     4081 accgcggtga tcgtccggct ggccccgttg aaggtgccgg tggcgcgcag gatcacgacc
     4141 ttgttgttgt ccagcgtggc gctggcgtcg agcgtgttgc cgctcccgat cagcgcctgg
     4201 tctccgttga ccgtgctcag gtcgttgcgc agcgtgaccg cgaaggtccc ggtggcgctg
     4261 gtcagccctg gaagcgccgt gctgctggtc aagaccttgc acgtgtaact gccgcagagg
     4321 ccgcccgtcg tcgtaccgct gcccagcagg gcggtgtcgt tgaaatcccg gccggcgagc
     4381 tgattgaagc cccactcgat tccgctctcg gcgagctgcc gggcccgggc gccatcggag
     4441 aggttgcgcg agatctgggg ctccaccgcc cccagggaca tgaacgtgag cgtgagcatc
     4501 gtcaggacca ccagcatgat catcgcgagg ggcagggcca ccccgcgctc attgccgagc
     4561 atcttgcgcc acgtcttcat tggtactggc ctccgcggta tgacttggct catcgatttc
     4621 gcagccgaac gcgatccacc atggtcacgt acgcgccccg ggtcgcggtc tggacggtca
     4681 tcgagatctc gacgctccgg atcagctcgg gggacgcggt gacgttgttg ttctcgtctc
     4741 gatacgtgaa ggtcagggcg gtgatgccgg acgcgatggt caccggagcg ctgtccacca
     4801 cgctttcctg gcgccgcagg ttgcctgcgg agagggagta ggtcacctgc tccccccggc
     4861 aggccgccgc cggcgccgcg cacgtcatcg gatcgttgac cagggcggcg gtgctgatag
     4921 gcgagcacgg aaccgcacac gtcgagccat cccagttgta ttgcagggtg agcgaggtcg
     4981 cggccgggct cgtgatgggc acgaacttgt agcagggaac gccggcggtg tagagcggat
     5041 agttcgtcgc gttcgggcaa gccgtggggt ccgtctcgcc gggcaagggc tcgtagccgg
     5101 cctcgcggac ctccttggcc aggcgctcga gggccacgcg cacggtctgc tggccgtcga
     5161 cctgggtcgt cccgaaccag tacgcccgct ggccctgcat cgtgagcccg agcaggctcg
     5221 ccatcacgag acccacgatg gcgcagacca ccagcatctc ggccagggtg aagccgcgct
     5281 gatcgttcat gagggctcct cggctcatcg attcgccagg aagatggact gcgacaccga
     5341 ccgctccgtg gtgagcacgc cgcgcccggt caccgggcgg tagaagacga cgacgtccac
     5401 gcgggcgccg agcgtaccgc ccgggttggt ggtgatcgtg gtggtccgcc ggtagctggg
     5461 cggcgcgccc gcgatcgtcc cgtaggcctc gctggccggg aggttggcga ccgtgagctg
     5521 caccaggccc gtctgccgca tggccagcgc cttggcctgc tcgatgcgct gctcggccag
     5581 gaacaccgcc gtgctctgct ggcgcccggc ctccacgcca tcgacgccga agccaaagcc
     5641 ggtcgccacc gcgacgagcc ccaccgcgat gatggcgcag gccaccagga tctcggccag
     5701 ggtgaacccg ggctctccgg ggcgagactt cttcaagcgg ctcagcacgt gcattgtcat
     5761 gtcctccatg tatccggtga ggaacagagc aagacgagtg ccagcctcga cccgcgagat
     5821 cgaccctggg ttctttgacg ataagtgttt gaaatatctg aattccacag gcctgtgcca
     5881 gggtggcgtt tctgcgggcg cacagggggt ctacaagttg tgacggatga cctacaggat
     5941 ccgtcagccg cgagacgggc ccggcccggg cgggccctca ggcctggtcg agccagacca
     6001 cctcgagctg agccccccgg tcgaagcagt tcttcggccc gtagttccgc acccgcggcg
     6061 cccatgacga tacgttgcgg gggtacggcg tgaacacgta gtagacctgg gccgcggcgc
     6121 gacgctgaat ctcgtcgatg atcttcttgc gcgacgacga cgaggtgaat cgccgctgcg
     6181 cctcgagcat cacgtcgagc tgcgcgtcgg ccacgtggct gcggttgccg acctggccgc
     6241 tccggaagaa gctggagaga tacccgtcca cctccgtgaa gagcgaggag ggcccccagc
     6301 ttgcctcctc gaaccgcccg aggaacgacc cgcggatgta ctgcccgtac tcctccggga
     6361 cgatctgcag ctccaccccg acccggcgca gatggccggc gaggcgctcg agatcctcca
     6421 cgtactcggg cccgtagccc ggccagctcg tgcacttgat cttgaggccg gcgggaaagc
     6481 cggcctcggc cagcagcttg cgggcccgca cgggatcgtg ctcgagatag cgggcaccct
     6541 cgccgaggtg ccgcgcggcc agcttccact gccgcatcgc gctcggcacc ggcccgtgag
     6601 cctcggcgcc gtcgccggcg agatattcgg ccacccacgt cttccggtcc accgcgaggc
     6661 tgagcgcgcg gcgcacccgc acgtcgttga acggcggccg gtcggcgcgc atggccaggg
     6721 agcgaatctc cagcccgtcc caggacacga ccgggtagcc ggggttcgcc ttcttgaagg
     6781 cggccacctc ggcgcgcggg atgcgcgcgt cgtgaccgag aaggtccacc tggcccgccc
     6841 ggaacagcgc gagctgggtc gggcggtcct tgatgaagag ccattcgacc ttgtccaggt
     6901 acggcagtcc gcgggcgtgg taggtgggat tgcgcgagaa gaccgccttc accccgggct
     6961 cgtaccgctc gagcacgaac ggcccgcaac cgatcaggga ctcggcggcc ttcggatcag
     7021 cgggccgatc ctccacttcc ggcggcagga tcgcggtcca gggctcggcc aggttgtgaa
     7081 cgaacggcgc gaaggtatcc gccaggtgga cgcgcaccgt gcgccggtcg ggcgcgtcga
     7141 tccgctccac cgggcccagg agcggcgcga agcacgacgt ccggagcgcg cgctcgagcg
     7201 agtgcttcac gtcggacgcg accagctcgc gcccgttgac gggcgggcgc gactcccagc
     7261 ggacgcccgg gcgcagcgcg atcgtgtagg tccgcccgtc cgccgacgcc accgcgctga
     7321 gcgcgagatc cggcgcgcgc gtgaagtccg acggcccgta ccgggccccg tggacgacct
     7381 tgaagagcgt gcggcgcacc agcgacgaca cgagctgggt ttgcggcgac gcggtggcgt
     7441 ggacgtcgaa ggtgggcggc tcgagaccga tcaccttcag cacgccaccc cggacgggcc
     7501 gctccgtcgc ccccgcccgg cgcgccgccg cggcggaggc ggcccccgcg gcgagcgcga
     7561 ggaaccggcg ccgcccgagc gcgccggcgg tcatggcagg ggctcgcagt ggttgagcag
     7621 gcgcagggac cagtgcccgc ccgcccgctc gagcacggag agggccgcgt aatcctggcc
     7681 cagcgcgagc agccgctcgg ggccgagccc gagggcatgg cagaggatcg cgcgattgct
     7741 cccgccgtgc gccaccaccg cgatcgtccc gcccgcgtgc cgcgccacca gtgcctcgaa
     7801 ggccgggacc gcgcgcgcga ccaggtccgg caggctctcc ccgccgggga actggaagcg
     7861 ccccacgtcg gccatccacg cctcgaacgc acgggcatcg cgcacgcgga tctgctcggc
     7921 ggtcagcccg tcccattccc ccatcgcgaa ctcccgcagg gccgggtccc ggacggggat
     7981 gagcgcgtgg ggcgcggcca ggatctcggc gctgcggcgg gtgcgctcga gatcgctgca
     8041 gtagatcgcg tcgaaggcga cgccgatcag gcggcgcgag agcgcatcga tctgagcgac
     8101 gccgagcgac gagaggggca cgtcgagatg cccgatgaac cggcgggtct ccgcgcccac
     8161 cacgctgccg tggcggacca ggcagacggt ggtggcgctc agagccaccc ccggtgcgcg
     8221 gccgccgccg cgccgagcag aacgccgagc tccgcgacct ccacgagagc gcccagcacg
     8281 tccccggtca gccccccgag gcggcgcgcc acgaagccgg ccgcgagcag cgccacgctc
     8341 catgccgccg ccgcgacggc cacgccccag agcccgagca gccacgcggc cagcgccccg
     8401 ccggccacca cgtgggccgg acccgtccag cgcgacagcc ccgcggcgaa cgcggcgccg
     8461 aggcccttgc cgggcgtggc gggcaccagc cagacgcccg cgagcagcgg cgccacccgg
     8521 ccgatcaccg gggccagcac gaggacgcgg aggcggagcg acggggagag cgcggcgagc
     8581 gcggtgacgg caagcaggca gagcaggacg agcccggcgg cgccgaacac gccgatgcgg
     8641 ctgtcacgca tgatggcgag ccgccccgcg gggtcgcggc ccgcgaggcc gtccagggag
     8701 tcggcgagcc catcgaggtg gatcccgccg gtggccacct tccaccccgt gacgaggagc
     8761 gcggcggcga ccagcggggg gaacgcgacg tccaccgcgc tcgcgacgac cgccagcgcg
     8821 ctccccagca gcagcccgac cacggggaac caccaggcgg cgcggcccag cgccgacggg
     8881 cccgccgatt cccggccggg aattgggacg accgtgagga agcggagcgc caggatcagc
     8941 gagcccatgc gggggccgcg gcggacacgc cgctcagacg ggcagaaccg gattgcgccg
     9001 cggcgggaac tcgagctgct tggagttcgc gtactccaga cgcttgatca gctcgtcgcg
     9061 cagctcggag ccgccgacga taccgtccac cagcatctcg gaggcgagct tgtagatgtc
     9121 cacgtcccgc gcgtactcgt cgcgcttctg ctggacgtag gccggccgct ccgcctccgg
     9181 cagctccatg atcttgttgt agtagaccgc gttgacggcc ggctccggcc ccatgatggc
     9241 cacctgcccc tgcggcaggg cgagggccgc gtccggctcg aaggccgggc cgcacatcgc
     9301 gtagagcccc gcgccgtagc acttgcgcac gaccacgcag atcttgggca cggtggcctg
     9361 cgaggtggcg aagacc
//
LOCUS       DASUFA010000098        11702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_710539, whole genome shotgun sequence.
ACCESSION   DASUFA010000098 DASUFA010000000
VERSION     DASUFA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11702
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710539"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFX87_03690"
     CDS             <1..479
                     /locus_tag="VFX87_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994041.1"
                     /translation="PSFILAMGIAAALGNSVTNIVIAIAITHVPIYARLIRGEMLRIR
                     EMEYAEAARTVGNRQRRIIFYHLLPNCFPPVIVQATLAMGFAILTVAALSYIGLGIRP
                     PTSEWGAMTAEGAGYIVSGEWWIFLFPGVAIMATVLSFNLVGDALRDVLDPRMKGV"
     gene            476..1459
                     /locus_tag="VFX87_03695"
     CDS             476..1459
                     /locus_tag="VFX87_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994042.1"
                     /translation="MTDLLALRDLTVHFDTDDGVVQAVDGAALRVGPGEVVGLVGESG
                     SGKSVTALAILRLLRPPARVVGGRIEFAGRDLLGLSEEEMRSVRGSQISMVFQSPRTS
                     LNPVLPVGRQIERLLALHGAAAPGQARERAVAMLREVGIAEPERRAGQYAHQLSGGMC
                     QRVMIAMALATNPRLLIADEPTTGLDVSIAAQILELLRDLGRRTGAAILLITHDLGVV
                     ARLCDRVVVMHAGQTVEWARVRGLFHTPAHPYTRALLRSIPRVDGAGAMEPIPGGVPS
                     LLAPPPGCRFASRCSLVQAECAAHVSVRTIGEDHAVACVAVEGAEWSGRGR"
     gene            1471..2421
                     /locus_tag="VFX87_03700"
     CDS             1471..2421
                     /locus_tag="VFX87_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994043.1"
                     /translation="MLSIERLQMHFPLRSLIPWRVPRVVHAVDGVSLRIAPGETLGLV
                     GESGCGKSTLARCVLRLIEPTEGRILLEDVDLTALRGEELRRRRRDVQMVFQDPTASL
                     NPRLSVAATVEEPLALHTELTGRARRVRADEVLEEVGLGAGLRDRYPHQLSGGQRQRV
                     NIARAIATHPRLVVLDEPTSALDVSLRARVILLLGELKRRLGMTYLFISHDLSTVRYL
                     CDRVAVMYLGVVVEEAPAARLFGHPAHPYTRALLSCIPVPDPDVTPERLTFAGEVPSP
                     IDIPAGCRLRGRCPLAQPVCAEPVPVREIAPGHTVACHLV"
     gene            2466..2789
                     /locus_tag="VFX87_03705"
     CDS             2466..2789
                     /locus_tag="VFX87_03705"
                     /inference="COORDINATES: protein motif:HMM:NF023070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994044.1"
                     /translation="MAVVRYVLAKMKPGVTREEYEKFEREVDYVVAGRLKTIVSYRTH
                     RITAADARLAGGPWDYIERIEITDRAAYEKELAASGKDLIDELYAKYLDRSYTTSIWS
                     ELVEP"
     gene            2789..3553
                     /locus_tag="VFX87_03710"
     CDS             2789..3553
                     /locus_tag="VFX87_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994045.1"
                     /translation="MMLAGRVALVTGASHGTGVDIARALAAEGARVAVHYRSDRAAAE
                     VLASAIRAGGGVAEAFAADVARSEEVRRLMAEVELRLGALGIVVNNAGPFNDTAFSEL
                     AEADWDYVMNANLKAVYLTSQLAAPGMRRAGWGRIVNIGATSGLVRSHGVYGLAKAAL
                     LPLTQALALELAPAVTVNAVVPSQIASARTDRMAAYKAAAIAGTPLGRLVTQAEIGQM
                     VLRLCSPEFDFVTGQAIVMDGGRVIPRFPRLNLSSE"
     gene            3570..4658
                     /locus_tag="VFX87_03715"
     CDS             3570..4658
                     /locus_tag="VFX87_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013385593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HEX4994046.1"
                     /translation="MTTTSLSARQIESLALGAWILGTGGGGSPYHGLLNLRRLYKEGA
                     RVRLMDPADLADDAAVAVVSTMGAPLVMQERVVDPGPMVESIRAMEAYLGRPFDAVMA
                     VEIGGSNALQPCQIGAVTGLPVVDGDAMGRAFPEAQMTSFAIHDLPMAPLTLVDVRDN
                     AVIVARAASWKWMERLSRKACVEVGSIAATCKAPRTGREVKECGILYTVSKAIRIGET
                     VQAAQAAHRDPIQAVIDAEGGRLIFRGKIRDVERRTTEGFLRGSATLDGLDGDRGRSF
                     RLAFQNEFAVGWLDDRPVVMTPDLICVMDALSGEAIGTETLRYGQRVAVVALPAAPIL
                     LTPKGLAHVGPRAFGYDLDFQSVFAEAR"
     gene            4655..6205
                     /locus_tag="VFX87_03720"
     CDS             4655..6205
                     /locus_tag="VFX87_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4994047.1"
                     /translation="MNRIGIDVGGTNTDAVLLEDDRVQHAVKTPTTADVMSGIRTALA
                     RLLEQAGGTPVVDAVMIGTTHFTNAVVQRRHLARVAAVRIGLPASASLPPFVDWPEDL
                     ADLVRGQVHMLEGGHEYDGRPIVPFDAAGMREVARRIRDAGIGSVAVASVFSPLNGSC
                     ELEAEAILQEICPGVAITLSHRLGRLGLLERENVTLLNACLRDLARSTTRAFVDALAA
                     SGIRAPLYLTQNDGTVMLASEAEAYPVYSFASGPTNSMRGAAFLSKREDAIVVDVGGT
                     TSDIGSLRHGFPRDANGVAEVGGVRTLFRMPDLLSLGLGGGSLVTEEPLAVGPRSVGY
                     LLTEQGRVFGGDRLTATDVAVAANLIELGDRRRVATLSPALVRACVVRMHAMIEEGVD
                     RMKADASPVPLIVVGGGSFLVPDQIPGISEVVHVPHQAVANAVGAAIAQVSGEVDQVF
                     QDLSRDRALAEAQRLAEAKATRAGADPTTLAVVEVEDLPLAYLPGNSLRVRLRVVGDI
                     ARGGAPRS"
     gene            6262..7533
                     /locus_tag="VFX87_03725"
     CDS             6262..7533
                     /locus_tag="VFX87_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX4994048.1"
                     /translation="MPPSARVLGLECLRCHTVFEGPGGFSGCPRCRQDGVSVNLTVRL
                     DLSPLAGLRPDGLPTTPRGLWRYRAVLPVASEHVVSLGEGATPLVHLERLGKRLGLPR
                     LFAKDESRNPTWSYKDRLCSVAVSHAVATGARVITISSTGNHGASTAAYAARAGLPCV
                     IFTLASVPETMKTLMQAYGAAVVACPTSESRWQLMREGIERLGWYPTSGFVIPPIGSN
                     PYGIEGYKTIAWEIAEDLGWTAPGVVVVPSAYSDGLYGVWKGWTELQALGLVKDVPRM
                     IAAEPFGPLAHALERGLEAPEVVPGGASVAFSIASRYGTWQGLAALKQSRGEGVRITD
                     EGIFEAQRALGREEGIFVEPSSATSLTAVMQLAGRRALDPAQPVVVVLTSGGLKDPGA
                     SRTWLPPVPDAPGDLDGVLRVLREQYGLRDL"
     gene            7572..8528
                     /locus_tag="VFX87_03730"
     CDS             7572..8528
                     /locus_tag="VFX87_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994049.1"
                     /translation="MGTDVPSRVTAWARAAERAGLGSAWIIEDYFHPGAYALAAAAAA
                     VTEHLAIGLGVVNPYTRHPALLAMETAALAGIAPGRVVLGLGSSNRQWIEAQMGIPFK
                     APLGGLRECVAIVRRLLAGERVTTRGEVFTVDGVKLEWCPPDAVPVLLGVKGPKALRL
                     AGEIADGVHCAVLSSPEHVRRVRQTAGAGRPRFTVVAYVIVAMSRDGHHARDAVRPLI
                     ARYLGFLHGQSILLDAGLGPDRTRPFREALGGAPAAHLVDDALIDALAVAGTPEHCRR
                     QLARWAEAGLDAPIAVLPRDVDMMEQIALCGEILVPAWREVR"
     gene            8534..9538
                     /locus_tag="VFX87_03735"
     CDS             8534..9538
                     /locus_tag="VFX87_03735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4994050.1"
                     /translation="MRFGVHIPTCIEGMMYPVPFAKPSDILPTALLCERLGFDSVWGN
                     DHMTTQRYVQKEFPDPPNFFEPLVTFAYCASRTIRLKFATGIIVLPMRNMPVLAKQVA
                     TLDQLSGGRVILGVGTGAYREEYEALFPDAQDVRRGTIVDEGMQALRLLFTERRATFR
                     GQAVHFEEVECFPKPAQDPMPIYAGGNHPEVRRRAGQYGQGWMPAVLSPEEIERGVAD
                     IHRAAAAAGRDGSTIDIAPQFAVSIGRTHDEAIRRFRASQLFKHLESLKRSTLREQTG
                     GFDQRNLIGSPAEISERIRVYERVGVTTLSAMLFVAGSVTEMQEAIELFGQEVLPNFH
                     "
     gene            9564..10739
                     /locus_tag="VFX87_03740"
     CDS             9564..10739
                     /locus_tag="VFX87_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX4994051.1"
                     /translation="MDPHVKSRLTKVLDAEGLDALVASTPENLQYVAGFRSIAHAIFR
                     GLELYGVFTREGVGLVIPFIDAAGVAADCIAVERLACYGKFFFNYAEPPGAVGARVRE
                     ITSAPAAGPGAALRDVLGGFGALGKRIGLDEAGVFPSTWQRLTGQLDGTTLVPAYGHF
                     RTARMVKSSGEVSRLERAALIAEDGVAAVFGMLKPGVTEREAATVFEQEVLRKGAQPF
                     FTVITMGERAALADVYPSERALAPGDLVRFDLGCVYQGYRSDISRTAVLGPPSEKQRR
                     YYDAVRDGEIAAIAAMKPGVSVSQIFETAMQVTRERGMPHYERHHVGHGIGLEPYDPP
                     TINAATHTALEPGMVFCVETPYYEHGWGGVQVEDAVEITPTGVRRLTQSSQELAILG"
BASE COUNT         1595 a         4049 c         4290 g         1768 t
ORIGIN      
        1 tcccctcgtt catcctcgcg atggggatcg cggctgcgct cggcaacagc gtgaccaaca
       61 tcgtgatcgc catcgcgatc acccacgtgc ccatctatgc gcggctcatc cgcggggaga
      121 tgctgcgcat ccgcgagatg gagtacgcgg aggcggcccg caccgtcggc aaccgccagc
      181 ggcgcatcat cttctaccac ctcttgccca actgctttcc gccggtgatc gtccaggcca
      241 cgctggcgat gggcttcgcg atcctcacgg tggccgcgct gtcgtacatc gggctcggca
      301 tccggccgcc gaccagcgag tggggggcga tgaccgcgga gggcgccggg tacatcgtct
      361 ccggggagtg gtggatcttc ctcttccccg gggtggcgat catggccacc gtgctgtcgt
      421 tcaacctggt gggcgacgcg ctgcgggacg tgctggaccc gcggatgaag ggcgtgtgac
      481 ggacctcctc gccctccggg acctcaccgt ccacttcgac accgacgacg gcgtggtgca
      541 ggcggtggac ggcgccgcgc tccgcgtcgg gccgggcgag gtggtggggc tggtggggga
      601 gtcgggctcc ggcaagtcgg tgaccgcgct cgcgatcctc cgcctgctgc gcccgcccgc
      661 gcgggtggtg ggcggccgga tcgagttcgc cggccgcgac ctgctcggcc tctcggagga
      721 ggagatgcgc tcggtgcggg gctcccagat ctcgatggtg ttccagagcc cgcgcacttc
      781 gttgaatccg gtgctgccgg tcggccgcca gatcgagcgg ttgctcgcgt tgcacggcgc
      841 ggcggccccc gggcaggccc gcgagcgggc ggtggccatg ctccgcgagg tcggcatcgc
      901 cgagccggag cgccgggccg ggcagtacgc ccaccagctc tcgggcggca tgtgccagcg
      961 ggtgatgatc gcgatggcgc tggccaccaa cccgcggctg ctcatcgcgg acgagcccac
     1021 taccggcctc gacgtctcga tcgcggccca gatcctcgag ctgctgcgcg acctgggccg
     1081 gcggaccggc gcggccatcc tgctgatcac ccacgacctg ggcgtggtcg cccggctgtg
     1141 cgaccgggtg gtcgttatgc acgcgggcca gaccgtcgag tgggcgcgcg tgcgcgggct
     1201 cttccacacc cccgcgcatc cgtatacccg cgccctgctc cgatccatcc cgcgcgtgga
     1261 cggggcgggt gcgatggagc ccatccccgg cggggtgccc tcactactgg cgccgccgcc
     1321 aggctgccgc ttcgcttctc gctgctcgct cgtgcaggcc gagtgcgcgg cgcacgtgtc
     1381 ggtgcggacg atcggggagg accacgcggt ggcgtgcgtc gcggtggagg gcgccgagtg
     1441 gtccggccgg gggagatagg cgtggacgcg atgctgtcga tcgagcgtct gcagatgcat
     1501 ttccctctgc gctcgctcat cccgtggcgg gtgccccgcg tggtccacgc ggtggacggc
     1561 gtctcgctgc gcatcgcccc cggggagacg ctgggcctcg tcggcgagtc gggctgtggc
     1621 aagtccacgc tcgcccgctg cgtgctgcgc ctgatcgagc cgaccgaggg gcgcatcctc
     1681 ctggaggacg tggacctcac cgcactcagg ggcgaggagc tgcggcggcg gcggcgcgac
     1741 gtccagatgg tcttccagga cccgaccgcc tccctcaacc cgcggctgag cgtggcggcg
     1801 acggtagagg aaccgctcgc gctacacacc gagctcaccg gccgagcccg gcgggtgcgg
     1861 gcggacgagg tgctggaaga ggtcgggctg ggggccgggc tgcgcgaccg gtacccgcac
     1921 cagctctccg gcggccagcg gcagcgtgtg aacatcgcgc gcgccatcgc cacccacccg
     1981 cgcctggtcg tgctggacga gcccacctcg gcgctcgacg tctcgctgcg ggcgcgggtg
     2041 atcctgctgc tgggcgagct caagcggcgg ctcggcatga cgtatctctt catctcgcac
     2101 gacctgtcca cggtgcggta cctgtgcgat cgcgtagcgg tgatgtacct cggcgtggtg
     2161 gtggaggagg cgccggccgc gcgtctcttc ggtcacccgg cgcatccgta cacccgcgcg
     2221 ctgctgtcct gcatcccggt cccggatccc gacgtcacgc cggagcgcct caccttcgct
     2281 ggtgaggtgc cgagcccgat cgacatcccg gccggctgcc ggctgcgggg gcgctgcccg
     2341 ctcgcccagc cggtctgcgc cgagccggtg ccggtgcgcg agatcgcgcc ggggcacacg
     2401 gtggcgtgcc acctggtctg actccgggcg cgcggttcac ccattcacga gcgaggaggc
     2461 agccgatggc cgtcgtgcgg tacgtgctgg cgaagatgaa gccgggggtc acccgggagg
     2521 agtacgagaa gttcgagcgg gaggtggact acgtggtggc ggggcggctc aagaccatcg
     2581 tgagctaccg cacccaccgg atcaccgcgg ccgacgcgcg gctggcgggc gggccgtggg
     2641 actacatcga gcggatcgag atcaccgacc gcgcggccta cgagaaggag ctggccgcgt
     2701 cgggcaagga cctgatcgac gagctctacg ccaagtacct cgatcggagc tacaccacgt
     2761 cgatctggtc ggagctggtc gagccgtgat gatgctggcc gggcgcgtcg ccctcgtcac
     2821 gggcgcctcg cacggtaccg gggtggacat cgcgcgcgcc ctcgccgcgg agggggcgcg
     2881 ggtcgcggtg cactaccggt cggatcgcgc cgccgcggag gtgctggcct cggccatccg
     2941 cgccgggggc ggcgtggccg aggccttcgc ggcggacgtc gcgcggagcg aggaggtgcg
     3001 ccggctgatg gcggaggtgg agctccgcct gggcgcgctc ggcatcgtgg tcaacaacgc
     3061 gggaccgttc aacgacaccg cgttcagcga gctggccgag gcggactggg actacgtcat
     3121 gaacgcgaat ctgaaagcgg tgtacctcac cagtcagctc gcggcccccg gcatgcggcg
     3181 ggcggggtgg ggccgcatcg tcaacatcgg cgcgacctcc gggctcgtgc gctcccacgg
     3241 cgtctacggg ctcgccaagg ccgcgctgct gccgctcacc caggcgctcg cgctcgagct
     3301 ggcccccgcg gtcaccgtga acgcggtggt gcccagccag atcgcgagcg ccaggaccga
     3361 ccggatggcc gcctataagg cggccgcgat cgcggggacg cccctgggcc ggctcgtgac
     3421 ccaggcggag atcggccaga tggtcctccg cctgtgctcg ccggagttcg acttcgtgac
     3481 cgggcaggcc atcgtgatgg acggcgggcg cgtgatcccg cgctttcccc ggctcaacct
     3541 ctcatcggag tgaagggagc ggcatcgaga tgaccaccac gtccctcagc gcccggcaga
     3601 tcgaatcgct cgccctcggc gcctggatcc tcggcacggg cggtgggggc agcccctatc
     3661 acgggctgct caacctgcgg cggctctaca aggaaggggc gcgggtgcgg ctgatggacc
     3721 ccgccgacct ggccgacgac gcggcggtgg cggtggtctc caccatgggg gcgccgctcg
     3781 tgatgcagga gcgggtcgtg gacccgggcc ccatggtcga gtccatccgc gcgatggagg
     3841 cgtacctcgg ccggccgttc gacgcggtga tggccgtcga gatcggcggg agcaacgcgc
     3901 tccagccatg ccagatcggg gcagtgaccg ggctcccggt ggtggacggc gacgccatgg
     3961 ggcgcgcgtt ccccgaggcg cagatgacga gcttcgccat ccacgacctg ccgatggccc
     4021 cgctcacgct cgtggacgtc cgcgacaacg cggtgatcgt ggcgcgggcc gcgagctgga
     4081 agtggatgga gcggctgagc cggaaggcct gcgtggaggt cggctcgatc gcggccacgt
     4141 gcaaggcgcc ccgcaccggg cgggaggtca aggagtgcgg catcctctac acggtgtcga
     4201 aggccatccg catcggcgag acggtgcagg cggcgcaggc cgcgcaccgg gacccgatcc
     4261 aggcggtgat cgacgcggag ggcggccggc tcatctttcg cggcaagatc cgggatgtgg
     4321 agcggcggac caccgaagga ttcctccgcg ggagcgcgac cctcgacggc ctcgacgggg
     4381 accgggggcg gtcgttccgc ctggccttcc agaacgagtt cgcggtcggc tggctcgacg
     4441 accggccggt ggtgatgacg cccgacctga tctgcgtcat ggacgcgctc tcgggcgagg
     4501 ccatcggcac cgagacgctg cgctacggcc agcgggtcgc ggtcgtggcg ctgcccgccg
     4561 cgccgattct cctgactccg aaggggctgg cccacgttgg cccgcgcgcc ttcggctacg
     4621 acctcgactt ccagtccgtg ttcgcggagg cgcgatgaac cgcatcggca tcgacgtggg
     4681 cggcaccaac acggacgcgg tgctgctgga ggacgaccgc gtccagcacg cggtcaagac
     4741 cccgaccacc gcggacgtga tgtcgggtat ccgcaccgcg ctcgcgcggc tgctcgagca
     4801 ggcgggcggg acccccgtgg tggacgcggt gatgatcggg accacgcact tcactaacgc
     4861 ggtcgtgcag cggcgccatc tggccagggt cgcggccgtc cggatcggcc tgcccgccag
     4921 cgcgtccctg ccgccgttcg tcgactggcc ggaggacctc gcggacctgg tgcgcgggca
     4981 ggtgcacatg ctggaaggcg gccacgagta cgacgggcgg ccgatcgtgc cgttcgacgc
     5041 ggccgggatg cgcgaggtcg cgcgccggat ccgggatgcc ggcatcggct cggtggccgt
     5101 cgcctcggtg ttctcgccgc tgaacggctc gtgtgagctc gaggcggaag cgattctcca
     5161 ggagatctgc cccggcgtcg ccatcaccct ctcgcaccgc ctgggccgtc tcgggctgct
     5221 ggagcgggag aacgtgaccc tgctcaacgc gtgcctgcgc gacctggccc gctccaccac
     5281 gcgcgccttc gtggacgcgc tggccgcgag cggcatccgg gctccgctct acctcaccca
     5341 gaacgacggc accgtgatgc tcgcctccga ggcggaggcc tacccggtct acagcttcgc
     5401 ctcgggcccg accaacagca tgcggggggc ggccttcctc tcgaagcgcg aggacgcgat
     5461 tgtggtggac gtcgggggaa ccacctcgga catcgggagc ctgcggcacg gctttccccg
     5521 cgacgccaac ggggtcgccg aggtgggcgg ggtccggacg ctcttccgca tgccggacct
     5581 gctctcgctg ggactggggg gaggcagcct ggtcaccgag gagccgctcg cggtcgggcc
     5641 gcggagcgtg ggctatctcc tcaccgagca gggccgggtg ttcgggggcg atcggctgac
     5701 cgccaccgac gtggcggtcg cggcgaacct gatcgagctg ggcgaccgcc ggcgggtggc
     5761 gaccctgtcg cccgcgctgg tgcgcgcgtg cgtggtgcgc atgcacgcga tgatcgagga
     5821 gggcgtggac cggatgaagg ccgacgcgag cccggtcccg ctgatcgtgg tcggcggcgg
     5881 cagcttcctg gtgcccgatc agatccccgg catctccgag gtcgtgcacg tgccccacca
     5941 ggcggtggcg aacgcggtgg gcgcggccat cgcgcaggtg agcggcgagg tggatcaggt
     6001 cttccaggat ctgtcgcgcg accgggcgct ggccgaggcg cagcgcctgg ccgaggccaa
     6061 ggccacgcgg gccggcgccg accccaccac gctcgcggtg gtggaggtcg aggatctgcc
     6121 gctcgcgtac ctgccgggca actcgctgcg ggtccggctg cgggtggtcg gcgacatcgc
     6181 gcgcgggggc gcgccgcgat cgtgaccgcg gcgcgtccgc gccttgacag gtcgagtcgg
     6241 cgtactacac taccgcgcgt catgcctcca tccgctcgag tcctcgggct cgaatgcctg
     6301 cgatgccaca ccgtgttcga gggccccggc ggcttctccg gctgtccgcg atgccggcaa
     6361 gacggcgtct ccgtcaacct caccgtccgt ctcgacctga gcccgctcgc gggcctgcgc
     6421 ccggacggat tgcccacgac gccgcgcggc ctctggcgct accgcgccgt gctgccggtg
     6481 gcgtccgagc acgtggtcag cctcggggag ggcgccacgc cgctcgtgca cctcgagcgc
     6541 ctcggcaagc gtctcggcct gccgcgcctg ttcgccaagg acgagagccg gaatccgacc
     6601 tggtcgtaca aggaccggct ctgctcggtc gcggtcagcc acgcggtggc cacgggcgcc
     6661 cgcgtgatca ccatctcctc taccggcaac cacggcgcct cgaccgccgc ctacgccgcc
     6721 cgcgccggcc tgccctgcgt gatcttcacg ctcgcctcgg tgcccgagac gatgaagacg
     6781 ctgatgcagg cctatggggc ggccgtggtg gcctgcccga cgtccgagtc acggtggcag
     6841 ctcatgcgcg aggggatcga gcgcctcggc tggtatccaa cgagcggctt cgtcattcct
     6901 ccgatcggct ccaaccccta cggcatcgag ggctacaaga cgatcgcgtg ggagatcgcg
     6961 gaggatctcg gctggacggc gcccggcgtg gtcgtggtgc cgagcgcgta cagcgacggg
     7021 ctctacggcg tctggaaggg ctggacggag ctgcaggccc tcggcctggt gaaggacgtg
     7081 ccgcggatga tcgccgccga gccgttcgga ccgctcgccc acgccctcga gcgcggcctc
     7141 gaggcgccgg aagtcgtgcc ggggggtgcc tccgtcgcct tctcgatcgc gtcgcgatac
     7201 ggcacgtggc agggcctcgc cgccctgaag cagtcgaggg gcgagggagt gcggatcacc
     7261 gacgagggca tcttcgaggc ccagcgcgcc ctcgggcgag aggagggcat cttcgttgag
     7321 ccctcgtcgg cgacgtcact caccgcggtc atgcagctcg ccgggcggcg ggcgcttgat
     7381 cccgcgcagc ccgtcgtggt ggtgctgacc tccggcgggc tcaaggaccc cggcgcgagc
     7441 cggacgtggc tgccccccgt cccggacgcg cctggcgacc tcgacggagt cctgcgcgtc
     7501 ctgcgcgagc agtacggtct gcgcgacctg tgatggggac tcgctgggcg accagtccgg
     7561 ggatcgccct catggggacc gacgtgccgt cgcgcgttac cgcgtgggca cgggctgccg
     7621 agcgggccgg gctcggcagt gcgtggatca tcgaagacta tttccacccc ggcgcctacg
     7681 ccctcgcggc cgcggccgcc gcggtcaccg agcatctcgc catcggcctg ggcgtggtca
     7741 atccctacac gcggcaccca gcgctgctgg ccatggagac ggccgctctc gccggcatcg
     7801 cgcccgggcg cgtcgtgctt gggctcggct cgagcaaccg gcaatggatc gaggcgcaga
     7861 tgggcattcc gttcaaggcg cccctcggcg gtctgcgcga gtgcgtcgcg atcgtccgcc
     7921 gcctgctcgc gggcgaacgg gtcaccacgc gcggcgaggt cttcaccgtc gacggcgtca
     7981 agctcgagtg gtgcccgccc gacgcggtcc ccgtgctgct cggcgtgaag gggccgaagg
     8041 cgctccggct cgcgggggag atcgccgacg gcgtgcactg cgcggtgctc tcgtctcccg
     8101 agcacgtgcg gcgggtacgc cagaccgcgg gagccgggcg cccgcggttc acggtcgtcg
     8161 cgtacgtcat cgtggcgatg agccgcgacg gccaccacgc gcgcgacgcg gtgcggccgc
     8221 tcatcgcgcg ctacctgggc ttcctccacg gccagtcgat cttgctggat gccggcctcg
     8281 ggcccgaccg cacccgaccg ttccgcgagg cgcttggcgg cgcccccgcc gcgcacctgg
     8341 tggacgacgc gctgatcgac gccctggccg tggcgggcac ccccgagcac tgccggcgac
     8401 agctggcgcg ctgggcggag gcggggctcg acgcgcccat cgccgtcctg ccgcgcgacg
     8461 tcgacatgat ggagcagatc gcgctctgtg gggagatcct agtgccggcg tggcgggagg
     8521 tcagatagag accatgagat tcggcgtgca catcccgacc tgcatcgagg gcatgatgta
     8581 cccggtgccg ttcgcgaagc cgtccgacat cctgccgacg gcgctgttgt gcgagcggct
     8641 cgggtttgac tcggtgtggg gcaacgacca catgacgacc cagcgctatg tgcagaagga
     8701 atttcctgac ccaccgaact tcttcgagcc cctggtgacc ttcgcgtact gcgcctcccg
     8761 cacgataagg ctcaagttcg ccaccggcat catcgtgctg cccatgcgca acatgccggt
     8821 gctcgccaaa caggtggcga cgctcgatca gctctcggga ggccgggtca tcctgggggt
     8881 ggggaccggc gcctaccgtg aggagtacga ggccctgttc cccgacgccc aggacgtgcg
     8941 gcgcgggaca atcgtggacg agggcatgca ggcgctgcgc ctgcttttca cggagcgccg
     9001 ggccacgttc cggggccagg cggtccattt cgaggaagtg gagtgcttcc cgaagccagc
     9061 gcaggacccg atgcccatct acgcgggcgg caaccacccg gaggtccggc ggcgcgccgg
     9121 gcagtacggc cagggctgga tgccggcggt gctgtccccc gaggagatcg agcggggggt
     9181 cgccgacatc catcgcgccg cggccgcggc cggcagggac ggctcgacga tcgacatcgc
     9241 gccgcagttc gcggtgtcga tcgggcgcac ccacgacgaa gcgattcggc ggttccgcgc
     9301 ctcgcaactc ttcaagcacc tggagtcgct caagcggtcc actctccgag agcaaactgg
     9361 tgggtttgac cagcgcaatc ttatcggcag cccggccgag atctcggagc gcatccgggt
     9421 gtacgagcga gtcggggtta ccacgctgtc cgccatgctg ttcgtggccg gttcggtgac
     9481 cgagatgcag gaggctatcg agctgttcgg ccaggaagtc ctgcccaatt tccactagca
     9541 cgcgcttgtc ccgggaggga gccatggatc cgcacgtgaa gtcgcgcctc accaaggtgc
     9601 tcgacgccga aggcctcgac gcgctcgtcg cgagcacgcc cgagaacctg cagtacgtgg
     9661 ccggattccg gtccatcgcc cacgccatct tccggggcct cgagttgtac ggggtcttca
     9721 cgcgcgaggg cgtgggcctg gtgatcccgt tcatcgacgc ggcgggcgtt gccgcggact
     9781 gtatcgcggt ggagcggctc gcctgctatg gcaagttctt cttcaactac gcggagccgc
     9841 ccggggcggt cggggcccgc gtgcgcgaga tcacgagcgc gcctgccgcg gggcctggcg
     9901 cggcgctccg cgacgtgctg ggcggcttcg gcgcgctcgg caagcggatc ggcctcgacg
     9961 aggccggcgt ctttccctcc acgtggcagc gactcaccgg gcagctcgac gggaccacac
    10021 tggtgcccgc gtacgggcac ttcaggactg cccgaatggt caagagctcg ggcgaggtga
    10081 gccggctcga gcgggctgcc ctgatcgccg aggacggggt tgccgccgtc ttcggcatgc
    10141 tcaagcctgg ggtcaccgag cgcgaggcgg ccaccgtgtt cgagcaggag gtgctacgca
    10201 agggggccca gccgttcttc accgttatca cgatgggaga gcgcgcggcg ctcgccgacg
    10261 tctacccctc cgagcgggcg ctcgcgccgg gcgatctcgt ccgcttcgac ctgggctgcg
    10321 tgtaccaggg ctatcgctcg gacatctccc ggaccgcggt cctcggtccg cccagcgaga
    10381 agcagcggcg atactacgac gcggtgcggg acggggagat cgcggccatc gcggcgatga
    10441 agcccggggt gtcggtcagc cagatcttcg agaccgccat gcaggtgacg cgcgagcgcg
    10501 gcatgcccca ctacgagcgg caccacgttg ggcacggcat cggactcgaa ccctacgatc
    10561 ctcccaccat caacgcggcg acccacaccg cgctcgagcc cgggatggtg ttctgcgtgg
    10621 agacgcccta ctatgagcac ggctgggggg gcgtccaagt cgaggacgcg gtcgagatca
    10681 cccccactgg cgtccgccgg ctcacccaat ccagccagga gctggcgatc ctcggctgag
    10741 agccgagcga ggagggaata agggaactct ctactcctcg atgttcgctc gtggcggtga
    10801 tccggggatc gagtccaagg cctagtgtcc cgaggaatat ctgtgagact tagtcagggt
    10861 cgttctcttg gagctcgcag gcgcgggact ggaatcctac cggaatttga atcccggggc
    10921 ctgcgatgtt gagtcaacat cacggaaccg gtcgatcaga ctaagagagc gcgtggcgga
    10981 cagcaaacac gggttcgaat cccgttgggg ccaccaaatt gtttctgttg atgtttgatc
    11041 ctccgggtgt gaggaacatt caagagttct tcaagctcgg aatggttttc ttcctcgcgg
    11101 gtccagcgac cccacggcag gcaccatcca cgcggtggag gcgtaacagg atggcctgtt
    11161 cgaacccgcg agcggtatga ggtgcgaggg acccgagcgg ctctggctgc tcgtctccgt
    11221 gcctcccggc ccggggcggc ttcgcctgga ctagtacttc gagctcttcg gtgagatcgc
    11281 gctgccgctg ccagccggcg cgggccccgt cgccagtgcc ccggccccgg gctgcgctag
    11341 ctcgctcgcc aagccacgcg gagcctctcg gggaagagtg gcccgcggag gggcgctgct
    11401 ggcgcgcgct acccttgggc cattcgacag ccgactcgaa cgacggtgtt cgccgaggtc
    11461 cggccacgcg aatgtgtggg cctctcgtgc atttgctcta gcccgggatg tcctctaggc
    11521 ttggccacaa atgatgacgc agaccgaacg cccgctctcc gacgacgagc gctggcaatt
    11581 gacggtgcgg gcgtcgaatg cccgcgccga gagtaggagg gcgctgttca agacgggcgg
    11641 cgcgagcgcg gccgtctgcg gcgtgcttgc gatcgtgacc ctcctcgcat ccggtgcacc
    11701 ac
//
LOCUS       DASUFA010000099         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1512340, whole genome shotgun sequence.
ACCESSION   DASUFA010000099 DASUFA010000000
VERSION     DASUFA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512340"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFX87_03745"
     CDS             complement(<1..909)
                     /locus_tag="VFX87_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4994052.1"
                     /translation="MKVAFAGVFAGHVADRVRARLRIPCEVTLVDDREIGDVDVLVSM
                     AFTREMAAAAPRLRLVQVPGAGLDRIDRAALAPGTALANAYGHDVGIAEYVIGAMLAI
                     TRSFCRLDAGLRRGRWDSVWSGAPVPLWPELAGKTLGILGYGRIGQAVARRALAFDMD
                     VLAIRRDASRPDPHRLAFLRGPAALAEVLGRADYLAVTLALTPDTRGLIGARQLALMK
                     PTAVLINVARGEVVDEDALYGALHRGAIAGAALDVWYRYPTGDAPVHPGHRPFHALPN
                     VLMTPHVSGWTEGMMDARAGVIAENIH"
     gene            complement(906..1274)
                     /locus_tag="VFX87_03750"
     CDS             complement(906..1274)
                     /locus_tag="VFX87_03750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4994053.1"
                     /translation="MAGTLSTLLGLRIVEASPDRVVAELTIRDELRTVGGALHGGTLM
                     ALADTVGAAATIVNLPPGAGTTTLESKTNFFAAGRSGVVRAEATPLHKGKRTHVWQTR
                     VTDEAGRLLSLTTQTQMVLM"
     gene            1416..2249
                     /locus_tag="VFX87_03755"
     CDS             1416..2249
                     /locus_tag="VFX87_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4994054.1"
                     /translation="MTTRTQATASRTTFGRLLKQWRERRRLSQLALAVDAEISSRHLS
                     FIETGRAQPSRDMVLLLSRVLEVPPRGRNDLLTAAGYAPVYRETGLEAPEMADVRRAL
                     DFMMRQQEPYPGLVIDGHWNILMTNAGARRLMGLFLDADAVAAVGGPPNAMRLFYHPR
                     GMRPFIVNWEATAAGLIQWLHRDLARGIGDAETGRLLEELLSYPDVPQKWRTLDLDAA
                     PVPFLAVELRRGNTHLTFFSTLTTLGVPYDITLHELRVECFFPADRASETLLRTLGDS
                     A"
     gene            2246..3295
                     /locus_tag="VFX87_03760"
     CDS             2246..3295
                     /locus_tag="VFX87_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994055.1"
                     /translation="MSASDRPTQFGRIHHPDEAWLAKQPPEPILEPELPIIDTHHHLW
                     DREGHHYLLTDFLADAATGHNLVATVFLECHSMYRAAGPVEMRPVGETEFVAGQAAMS
                     ASGMYGPTRVAAGIVGFADLTLGDRVEPVLQAHLRAGGGRFRGVRHSAAWDASDIIGN
                     SAVADGPHVYRRPDFRAGLAVLTRLGLSLDAWVFHPQLADVVDLARAFPDTTIILGHV
                     GGVLGYGPYTGKQKEVFAAWKPQMAELARCPNVVVKLGGMMMRLAAYDYRTQPKPPTS
                     TELAALWRPYMETCIELFGASRCLFESNFPVDKMGIGWAGLWNALKRIAGGASAAEKQ
                     AMFSGTARRVYRLDA"
BASE COUNT          494 a         1265 c         1176 g          480 t
ORIGIN      
        1 atggatgttc tccgcgatga ctccggcccg ggcgtccatc atcccttccg tccagcccga
       61 gacgtggggc gtcatcagca cgttcggcag cgcgtggaac gggcggtggc ctgggtgaac
      121 cggcgcgtcg cccgtcgggt agcgatacca gacgtcgagc gcggctcccg cgatcgcgcc
      181 gcgatggagc gcgccgtaga gcgcgtcctc gtccaccacc tcgccccggg ccacgttgat
      241 cagcaccgcg gtgggcttca tcagcgcgag ctgccgcgcg ccgatgagcc cgcgcgtgtc
      301 aggcgtgagc gccagcgtga cggccaggta gtcggcgcgc cccaggacct cggcgagcgc
      361 cgcgggtcca cgcaggaatg cgagcctgtg cggatcgggc cggctcgcgt cgcgccggat
      421 cgccagcacg tccatgtcaa aggccagcgc ccgccgggcg acggcctggc cgatgcggcc
      481 gtagccgagg atacccaggg tcttgcccgc cagctccggc cagagcggca ccggcgcgcc
      541 ggaccacacg ctgtcccagc ggccccggcg caggccggcg tcgaggcgac agaagctccg
      601 ggtgatcgcg agcatcgcgc cgatcacgta ctccgcgatg ccgacgtcgt ggccataggc
      661 attggccagc gcggtccccg gagcgagtgc ggcccgatcg atgcggtcga ggcccgcgcc
      721 gggcacctgc accaggcgga gccgcggggc ggccgcggcc atctccctcg tgaaggccat
      781 gctcaccagc acgtccacgt cgccgatctc gcggtcgtcg accagcgtca cctcgcaggg
      841 gatacgcagg cgcgcccgca cccggtcggc gacgtggccc gcgaagactc ccgcgaaggc
      901 gaccttcaca tgagcaccat ctgggtctgg gtcgtcaggg agaggagccg gccggcctcg
      961 tcggtgacgc gcgtctgcca cacgtgggtg cgcttgccct tgtgcagggg ggtggcctcc
     1021 gcgcgcacca ccccggagcg gccggctgcg aagaagttgg tcttcgactc gagcgtggtc
     1081 gtgcccgcgc ccggcggcag gttcacgatg gtggcggcgg cgcccaccgt gtcggccagc
     1141 gccatcaagg tcccgccgtg cagcgcgccc ccgacggtgc gcagctcgtc tcggatcgtc
     1201 agctcggcca ccacgcggtc ggggctcgcc tccacgatgc gcagccccag cagcgtgctc
     1261 agggttccgg ccatccgctc gttgaacacg gtgagatcga tgtccatggg tgacctccgg
     1321 cggccatcct caccgaaacg gggagccagt tcaattacct tggaggtaat ggcgctcgat
     1381 cccggcctgc gtatacttcc gggcatggtg ccgacatgac gaccagaaca caggcgaccg
     1441 cgagccggac gacgttcggc cgactgctca agcagtggcg cgagcgccgg cgcctcagcc
     1501 agctcgccct ggcggtggac gccgagatct cctcgcggca cctgagcttc atcgagacgg
     1561 gccgcgccca gccgagccgc gacatggtgc tgctgctgag ccgcgtgctc gaggtcccac
     1621 cccgcggccg caacgacctg ctcaccgccg ccggctacgc gccggtgtac cgggaaaccg
     1681 gcctcgaggc gcccgagatg gccgacgtgc ggcgggcgct cgacttcatg atgcgccagc
     1741 aggagcccta ccccggcctg gtcatcgacg gccactggaa catcctgatg accaatgccg
     1801 gcgcccggcg gctgatgggc ctgttcctcg acgccgacgc ggtcgcggcg gtgggcggcc
     1861 cgcccaacgc gatgcgcctc ttctaccatc cgcgcggcat gcggccattc atcgtcaact
     1921 gggaagcaac cgcggccggc ctcatccagt ggctccaccg cgacctggcc cgcggtatcg
     1981 gggacgcgga gacgggccgg ctgctcgagg agctgctctc ctacccggac gtcccgcaga
     2041 agtggcgcac gctcgatctc gacgccgccc cggtgccgtt cctggccgtc gagctccgcc
     2101 gcgggaacac ccacctcacg ttcttctcca cgctgaccac gctcggcgtc ccctacgaca
     2161 tcacgctgca cgagctgcgc gtggagtgct tctttcccgc cgatcgggcc agcgaaaccc
     2221 tgctgcgcac cctgggagat tccgcatgag cgcatccgat cgtccgaccc agttcggcag
     2281 gatccaccat ccggacgagg cgtggctggc caagcagccg cccgagccca tcctcgagcc
     2341 cgagctgccg atcatcgaca cccatcacca cctgtgggac cgagagggcc atcattacct
     2401 gctgaccgac ttcctcgccg acgcggcgac cggccacaac ctggtcgcca ccgtgtttct
     2461 cgaatgccac tcgatgtacc gcgcggccgg gcccgtggag atgcgcccgg tgggcgagac
     2521 cgagttcgtg gccggccagg ccgcgatgag cgccagcggc atgtacggcc ccacccgggt
     2581 ggccgccggc atcgtgggct tcgccgatct cacgctcggc gaccgcgtgg agccggtgct
     2641 gcaggcgcat ctccgcgccg gcggcgggcg cttccgcggg gtgcggcact cggcggcctg
     2701 ggacgccagc gacatcatcg gcaacagcgc ggtggccgac gggccgcacg tctaccggcg
     2761 gccggacttc cgggcgggtc tggccgtgct cacccggctc gggctctcgc tcgacgcctg
     2821 ggtgttccat ccgcagctcg cggacgtggt ggacctggcc cgcgcgtttc ccgacacgac
     2881 catcatcctc ggccacgtcg gcggcgtgct cggctacggc ccctacaccg gcaagcagaa
     2941 ggaggtgttc gcggcctgga agccccagat ggccgagctc gcccggtgcc ccaacgtggt
     3001 cgtgaagctg ggcggcatga tgatgcgcct ggccgcctac gactaccgca cccagccgaa
     3061 gccacccacc tcgaccgagc tggccgccct ctggcgcccg tacatggaga cgtgcatcga
     3121 gctcttcggc gcgagccgct gcctgttcga gagcaacttc ccggtggaca agatgggcat
     3181 cggctgggcg gggctctgga acgcgctcaa gcggatcgcc ggcggcgcct ccgccgcgga
     3241 gaagcaggcc atgttcagcg gcaccgcccg gcgggtctat cgcctggacg cgtagacgtg
     3301 gacgagtacc gcgaggccaa ccgggcgctc tggaacgcct ggacgcggat caacctccag
     3361 tccgagatgt acgacgtcga ggccttcgcg gcgggccgcg ggcgcgacct cgatt
//
LOCUS       DASUFA010000100         7587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_355284, whole genome shotgun sequence.
ACCESSION   DASUFA010000100 DASUFA010000000
VERSION     DASUFA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355284"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFX87_03765"
     CDS             <1..401
                     /locus_tag="VFX87_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX4994056.1"
                     /translation="RLAATGLTPVLLGTGDDAPTATAVARAAGGPIASLVGRDRPELL
                     PRLLARLRCLVSGDTGVAHRAAAVDVATVTLFGPTDRRLTAPRGRKSRPLDRAAPCAP
                     CFLPRCPIDHVCLTRIEPDEVLHEVRQAVA"
     gene            398..1477
                     /locus_tag="VFX87_03770"
     CDS             398..1477
                     /locus_tag="VFX87_03770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4994057.1"
                     /translation="MKTVMHTEASPGLGGQEIRTLNEARWTVERGWRVLVVGQPDGRL
                     LERARATGLETAPVRMTGPLDLRALVGLVRLIRRERVSLVHTHSSIDAWLGGMAARLC
                     RVPVVRTRHVSIAIRRGLNPVYRWLADRVITSGEAIRRLVVEVGVPPERVIAIPAGVN
                     LESFPFGARAPETARELGLGRPVIGSVAMFRGSKGHPQLLQAFAMVREKRPGATLLLV
                     GDGIRRRWVEQLAREAKLSDAVVFTGFRPDVPALLATMDCFAQASTRTEGVPQALLQA
                     FATGVPVVASRIGGIPEVVADGETGLLVESESVEALAAGIERVLDDPEAAAHRARAAR
                     ALVEARFSHIHAIDRLLQVYDELLA"
     gene            1474..2607
                     /locus_tag="VFX87_03775"
     CDS             1474..2607
                     /locus_tag="VFX87_03775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4994058.1"
                     /translation="MSPSRPLTVLHLVANRWWTGSAEPVVRLLTGLRARGHRVLLGLI
                     AGDRFEQKAREAGLDPLPGLSLEVRGNPLHAVSDLRGLRAIVRAEGVDVIHTHHSHDH
                     WLGWLARGGGPAALVRTFHNARAVRLDWPSTALYRRTEALIAVSREIEERGGGVGIPA
                     DRLARVNGVVDLGRFEIGDGGAAIRKEFDLGGAPVLGCVARLASRRGHELLIRSFRLL
                     LAAYPGARLLLVGKGEARARLEALVSELGLTREVLFTGYRDVDLPGVLQALDAFALMG
                     AGSDESCRAVLEAMAAGRPVVGRRVGALPETVLHGVTGLLVDDDRPESVAAALRAMIA
                     EPERAAAMGKAGRERARALYGSDAHAARMEEIYESALRLRNTR"
     gene            2786..3859
                     /locus_tag="VFX87_03780"
     CDS             2786..3859
                     /locus_tag="VFX87_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4994059.1"
                     /translation="MELERARHDVTLVCKKSSQARVMSRAKEAGVERVETLHLSSGVS
                     PIMDGRDLGQLAAWLPETQVFHVHRGKEHWLAALANRMSNPRRAIVRTRHIVQPIRPH
                     ALNRWLYGSATDLVVTVTEAIRRQLIASGLAPEDRVVALPGGVDVERYRPDVPPARGL
                     DARGLLMVSPDVPLIGLVGGFRVMKGHETVVAAAARVAATGLPFHLVFIGQGPFAERV
                     RGLVSAAGLADRVSLVGFVDELPMVMAALDVALYAALESDGMSRVLWEYLASAVPVVA
                     TRVGVVPEVLEDDVTALLVPAGESVPLAAAIERLLRDAALRRRLGAAGADLVRERFSG
                     ARLAEHLTALYLSLAVGSARPSR"
     gene            3856..4989
                     /locus_tag="VFX87_03785"
     CDS             3856..4989
                     /locus_tag="VFX87_03785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4994060.1"
                     /translation="MKVSILCFDVSDNAMGRAWLLARLLEPIGAVEIVGPRFGAAVWE
                     PVADEPVPVRSIQGGRLPAFAARMPALARMADGDLIYASKPRLTSAGIGYLARLGRRR
                     PLLLDIDDWEIGFFLRGGVWGTLGRALNLSNPRGLAWTWLMERMRGIADGVTVASRFL
                     EQRFGGVLIPHVRDTDRWAPGRAEPGPARDRLGVDKERVVMFLGTPRGYKGVEDLADA
                     VGRLGRPDVVLALVGTAPTSDTGRALSARHPGIRLVGRIPITQVPGFLSAADVVVVPQ
                     RESSDTRGQVPAKLFDAMALGRPIVSTRVSMIPEILEGCGALVPPGDVAALASAIRRL
                     LDRPDEARALGEAARRRAVDRYSFESARRALFPLVERVRRMGR"
     gene            4989..6089
                     /locus_tag="VFX87_03790"
     CDS             4989..6089
                     /locus_tag="VFX87_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4994061.1"
                     /translation="MRLGLICRPFSFHGGVETATAGLLTALRRAGHDVELISTRRQAS
                     VPGLVVRRVPTLRHPSVLRLISFMVAARAAAARHGYDLVQSHERVLQQDLYRAGEGSH
                     AAYLEAMGRTVAGLNPYHRLVLALERRIFQLRTARHVVAISRQGKEEIERRYGTDPER
                     VTLIYNGVDLERFHPDNRGRHRRPTREALGIPEEAWTVLFVGSGFERKGLGPLLEAFA
                     RVADAGSRLVVTGKGDAARYQSLAARLGIAERTIWTGPREQVERLYAAADAVALPARY
                     EPFGNVHLEALASGVPMLSSARAGGSEIVRRGENGWVAAEPTVDPIAEGLAALRDAPA
                     GGWASRARASVERFTYAAQAQAFTALYHLLRR"
     gene            6210..6776
                     /locus_tag="VFX87_03795"
     CDS             6210..6776
                     /locus_tag="VFX87_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4994062.1"
                     /translation="MDRDGCLTEEVGYVNHPSRIRLLPRTAAAVRRLNQAGVAAVMVT
                     NQAGVARGYFSEETLHAANRRMVGELEAGGARLDGLYVCMHHPREGQPPYRADCDCRK
                     PRPGLLTRAATELGLDLRTSVMVGDRLSDVAAGQSAGTAGVLVLTGYGRGEWELRPPG
                     GEIKPDHVADDLLDAVAWALVRVADSGR"
     gene            6778..>7587
                     /locus_tag="VFX87_03800"
     CDS             6778..>7587
                     /locus_tag="VFX87_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX4994063.1"
                     /translation="MFPVQRLRDAVARFPGKRIAVIGDLIADEYLYGKPARISREAPV
                     LILRFTDREVRLGGAANAAHNVHALGATVLPVGLLGQDGAGAEVHALFHQAGMSTDGI
                     SREPARPTPVKTRIMAGGYESTRQQVVRLDREPDGGISGPAEARLIERLQAVGADADA
                     FLVSDYGYGTVTPRVYETVLELGRARQVPVTVDSRYELPRFKGATAATPNEPEVEELT
                     GQELGDEPSVEKAGRAVLERLDARLLLVTRGSRGMALFEREGPVTFMPIHGS"
BASE COUNT          910 a         2799 c         2800 g         1078 t
ORIGIN      
        1 agcggctcgc cgccaccgga ctcacgcccg tcttgctggg caccggagac gacgcgccca
       61 ccgctaccgc ggtcgcccgg gcggccggcg ggccgatcgc gtcgctcgtc gggcgcgacc
      121 ggcccgagct gctgccgcgc ctgctcgcgc gtctgcgctg cctcgtgagc ggcgacaccg
      181 gcgtcgcgca ccgggccgcc gcggtcgacg tggccaccgt cacgctgttc ggacccaccg
      241 accgccggct caccgccccg cgcggccgca agagccggcc cctcgaccgc gccgccccgt
      301 gcgcgccgtg ctttctccct cgctgtccca tcgaccatgt ctgcctcacc cggatcgagc
      361 cggacgaggt gctgcacgag gtcaggcagg ccgtggcgtg aagaccgtga tgcacacgga
      421 agcctcgccg ggtctgggtg gccaggagat acgcacgctc aacgaggcac gctggacggt
      481 ggagcgcggc tggcgcgtgc tggtggtcgg ccagcccgac gggcgtttgc tggagcgggc
      541 gcgcgccacc ggcctcgaga ccgcgcccgt ccgcatgacc ggtccgctcg atctgcgcgc
      601 gctggtcggc ctggtgcggc tgatccggcg cgagcgggtg agcctggtgc acacgcacag
      661 ctccatcgac gcctggctcg gcggcatggc ggcccggctc tgccgcgtgc cggtggtgcg
      721 cacccggcac gtctcgatcg ccatccggcg gggcctgaat ccggtctacc gctggcttgc
      781 cgaccgcgtg atcacgagcg gcgaggccat ccgacggctg gtcgtggagg tgggcgtgcc
      841 gcccgagcgg gtcatcgcga tcccggccgg cgtgaacctc gagtccttcc cgttcggcgc
      901 ccgcgcgccg gagacggcgc gcgagctagg cctcggccgc ccggtcatcg gctcggtcgc
      961 gatgttccga ggctccaagg gccacccgca gctcctccag gccttcgcga tggtgcgcga
     1021 gaagcggccc ggagccaccc tcctcctggt cggcgacggg atccggcgcc gctgggtgga
     1081 gcagctcgcc cgcgaggcaa agctctcgga tgcggtcgtc ttcaccgggt tccgccccga
     1141 cgtgccggcc ctgctcgcca ccatggactg cttcgcccag gcctccacgc gcaccgaggg
     1201 cgtgccgcag gccctgctgc aggccttcgc caccggcgtg ccggtggtgg cgagccggat
     1261 cggcggtatc cccgaggtgg tggccgacgg cgagaccggg ctgctcgtcg agagcgaatc
     1321 ggtcgaggcg ctcgccgccg ggatcgagcg cgtgctggac gaccccgagg cggccgcgca
     1381 ccgggcacgc gcggcgcgcg ccctcgtgga ggcgcggttc tcgcacatcc acgcgatcga
     1441 ccggctgctc caggtctacg acgagctgct cgcatgagcc cgagccggcc gctcaccgtg
     1501 ctccacctcg tggccaatcg ctggtggacg ggcagcgccg agccggtggt ccgcctgctg
     1561 accgggctgc gggcgcgcgg gcaccgggtc ctcctcggcc tgatcgcggg cgaccgcttc
     1621 gagcagaagg cgcgcgaggc cggcctcgac ccgctgcccg ggctctccct cgaggtccgg
     1681 ggcaacccgc tccacgcggt ctcggacctg cgcggcctgc gcgccatcgt gcgggccgag
     1741 ggcgtggacg tgatccacac gcaccactcc cacgatcact ggctgggctg gctggcgcgc
     1801 ggcggtggcc ccgccgcgct cgtccgcacc ttccacaacg cccgcgcggt gcgcctcgac
     1861 tggccgtcca ccgcgctcta ccggcgcacc gaggcgctga tcgcggtgag ccgcgagatc
     1921 gaggagcgcg gcgggggcgt cggcattccc gccgaccgtc tcgcgcgggt gaacggcgtg
     1981 gtggacctcg ggcgattcga gatcggtgac ggcggcgccg ccatccgcaa ggagttcgac
     2041 ctgggcggcg cgccggtgct cggctgcgtg gcccgcctcg cgtcccgccg cggtcacgag
     2101 ctgctgatcc gcagcttccg gctgctcctc gcggcatacc cgggggcgcg gctcctcctg
     2161 gtcggcaagg gcgaggcacg ggcgcgtctc gaggccctcg tcagcgagct cgggctcacg
     2221 cgcgaggtgc tgttcaccgg ctatcgcgac gttgatctgc ccggcgtgct ccaggcgctc
     2281 gacgcgttcg cgctgatggg cgcgggctcc gacgagtcct gccgcgcggt gctggaggcc
     2341 atggcggccg ggcgcccggt ggtcggccgc cgcgttggcg cgctgcccga gaccgtgctc
     2401 cacggcgtca ccgggctgct tgtggacgac gaccggcccg aatccgtcgc ggcggccctg
     2461 cgcgccatga tcgcggagcc ggagcgggcg gccgcgatgg ggaaggcggg gagggagcgg
     2521 gcgcgggcac tctacggctc cgacgcacat gcggcgcgga tggaggagat atacgagagc
     2581 gcgcttcggt tgcggaacac gcgatgagct cctgcggaca ctcgtttagt tctttaagct
     2641 ctatcatatg cgggggccca gaaatggccc ccgcactcct ccagctaggg gacaacccgc
     2701 gcgattggtc cccttcgtga cgatcaggca tatcgtttcg tgtcgggggt ggtcttcgga
     2761 tgcgtactgg gccgcgcggg cggtggtgga gctcgagcgt gcgaggcacg acgtgacgct
     2821 cgtctgcaag aagagcagcc aggcccgcgt catgagccgc gcgaaggagg cgggggtcga
     2881 gcgggtcgag acactgcatc tgtcgagcgg cgtctcgccg atcatggacg gccgcgacct
     2941 gggccagctc gccgcgtggc tgccggagac gcaggtattc cacgtccatc gcggcaagga
     3001 gcactggctg gccgcgctcg ccaaccggat gtcgaacccg cgccgggcca tcgtgcgcac
     3061 gcgccacatc gtgcagccca tccggccgca cgcgttgaac cgctggctct acggctccgc
     3121 caccgatctc gtcgtcacgg tcaccgaggc catccgccgc cagctcatcg ccagcgggct
     3181 ggccccggag gaccgggtgg tggcgttgcc gggcggcgtg gacgtcgagc gctatcgccc
     3241 ggacgtgccg ccggcgcgcg gcctcgacgc gcgcggcctg ctgatggtgt cgccggacgt
     3301 cccgctcatc ggcctggtcg gcggcttccg ggtcatgaag ggccacgaga cggtggtcgc
     3361 ggcggcggcc cgggtcgccg ccaccggcct cccgttccac ctggtcttca tcggccaggg
     3421 gccgttcgcc gagcgggtgc gcggtctcgt cagcgcggcc gggctcgccg accgcgtctc
     3481 cctggtcggc ttcgtggacg agctgcccat ggtcatggcg gcgctcgacg tggcgctcta
     3541 cgccgcgctc gagtccgacg gcatgtcgcg ggtgctctgg gagtatctgg cctcggccgt
     3601 acccgtggtg gcgacccggg tgggtgtggt gcccgaggtg ctggaggacg acgtcaccgc
     3661 gctcctggtg cccgccggcg agtccgttcc cctcgcggcg gccatcgagc gcctcctgcg
     3721 cgacgcggcc ctccggcggc gtctgggcgc cgccggcgcc gatctggtgc gcgagcggtt
     3781 ctcgggcgcc cggctcgccg agcatctgac cgcgctctac ctctcgctcg cggtcggctc
     3841 ggcgcggccg tcccggtgaa ggtctcgatt ctctgcttcg atgtctcgga caacgcgatg
     3901 gggcgcgcgt ggctgctcgc gcgcctgctg gagccgatcg gagcggtgga gatcgtcggc
     3961 ccgcgcttcg gcgcggccgt gtgggagccc gtggcggacg agccggtacc ggtccgctcg
     4021 atccagggtg ggcggctccc ggccttcgcc gcgcgcatgc cggccctcgc ccgcatggcc
     4081 gacggcgacc tgatctacgc gtcgaagccc aggctgacca gtgccggcat cggctatctc
     4141 gcccgcctcg ggcggcgccg accgttgctc ctcgacatcg acgactggga gatcggcttc
     4201 ttcctccgcg gcggcgtctg ggggactctc gggcgagcgc tgaatctttc caatccacga
     4261 ggccttgcct ggacgtggct catggagcgc atgaggggaa tcgccgacgg cgtcacggtt
     4321 gcctcgcgct tcctcgagca gcgattcggc ggggtcttga tcccccacgt ccgggatacg
     4381 gaccgctggg cgccgggccg ggccgagccg gggcccgcgc gggatcggct gggcgtggac
     4441 aaggagcgcg tcgtcatgtt cctcgggacg ccccggggct acaagggcgt ggaggacctg
     4501 gccgacgcgg tgggccggct cgggcggccc gacgtcgtcc tggccctggt cggcacggcg
     4561 cccacctcgg ataccggccg tgccctgagc gcccggcatc cgggcatccg cctggtcggg
     4621 cggatcccca tcacgcaggt cccgggcttt ctctccgccg ccgacgtggt cgtcgtcccc
     4681 cagcgggaga gcagcgacac gcgcggccag gtgcccgcga agctcttcga cgccatggcc
     4741 ctcggccggc cgatcgtgtc cacgcgggtc tccatgatcc cggagatcct cgagggctgc
     4801 ggggcgctgg tgccgccggg cgacgtggcc gcgctcgcct cggccatccg gcgcctgctc
     4861 gaccggcccg acgaggcgcg tgcgctgggg gaagcggccc ggcgccgggc ggtggaccgc
     4921 tacagcttcg agtccgcgcg ccgcgctctc ttccccctgg tcgagcgggt acgccggatg
     4981 ggacgctgat gcgcctgggt ctcatctgcc ggccgttttc cttccacggc ggcgtcgaga
     5041 ccgccaccgc gggccttctc accgcgctcc gccgcgccgg ccacgacgtc gagctgatca
     5101 gcacgcgacg gcaggcctcg gtgcccggcc tcgtggtccg ccgcgtgccg acgttgcggc
     5161 acccctccgt gctccgcctg atctcgttca tggtggcggc acgagccgcg gcggcccgtc
     5221 acgggtacga cctcgtgcag agccacgagc gggtgcttca acaggatctc tatcgcgcgg
     5281 gcgagggctc tcacgccgcc tacctcgagg cgatggggcg aacggtcgca ggcctcaacc
     5341 cgtatcaccg cctggtcctg gccctcgagc gacgcatctt ccagctccgc acggcgcgcc
     5401 acgtggtcgc gatctcgcgg cagggcaagg aggagatcga gcggcgctac ggcaccgatc
     5461 cggaacgggt gacgctcatc tacaacggcg tggacctcga gcgcttccat cccgacaacc
     5521 ggggccgcca ccgccgcccg acacgcgagg cgctgggcat tcccgaggag gcgtggaccg
     5581 tgctcttcgt gggctcgggc ttcgagcgca agggcctggg cccgctgctg gaggccttcg
     5641 cgcgcgtggc ggacgcggga agccggctgg tcgtgaccgg gaagggggac gcggcgcgct
     5701 accagagcct ggccgcgcgc ctcggcatcg ccgagcgcac gatctggacc gggcccaggg
     5761 agcaggtgga gcgcctctac gccgccgccg acgcggtcgc gctgcccgca cggtacgagc
     5821 ccttcggcaa cgtgcatctg gaagccctcg cctccggcgt gccgatgctc agcagcgcgc
     5881 gtgccggcgg ctccgagatc gtgcgccgcg gggagaacgg ctgggtcgcc gccgagccca
     5941 ccgtcgaccc gattgccgag ggcctcgccg cgctccgcga cgcgccggcg ggtggctggg
     6001 cgtcgcgcgc gcgggcctcc gtcgagcggt tcacctacgc cgcgcaggcc caggcgttca
     6061 ccgccctcta ccacctcctc cgacgctgag cgccggtgcc cgccgggcac acgctccaaa
     6121 ccctcgattt tcattgatca atcggggtcg cggccttaca ctgcgagtta atgtcgagcc
     6181 atgctgggca tctgcggcct gccgtgttca tggaccgcga cggctgtctc acggaggagg
     6241 tgggctacgt caaccacccg agccggatcc gattgctgcc gcgcaccgcg gcggcggtcc
     6301 gccgactcaa ccaggcgggc gtcgccgcgg tgatggtcac gaaccaggcg ggtgtcgccc
     6361 gcgggtactt ctccgaggag acgttacacg cggccaatcg ccgcatggtc ggcgagctcg
     6421 aggcgggagg cgcgcgcctc gacggcctct acgtctgcat gcaccaccca cgcgagggcc
     6481 agccgcccta ccgggccgac tgcgactgcc gcaagccgcg ccccggactc ctcacgcgtg
     6541 cggccaccga gctcggcctc gatctgcgca cctcggtgat ggtaggcgac cggctctccg
     6601 acgtggcggc cggccagtcc gcgggcacgg caggggtgct cgtgctcacc ggctacgggc
     6661 gcggggagtg ggagctacgc ccgcccggcg gcgagatcaa gccggaccac gtggccgacg
     6721 atctgctcga cgcggtggcc tgggcgctcg tccgcgtcgc cgactcgggg cgctgacgtg
     6781 ttcccggtac agcggctcag ggacgcggtg gcccgcttcc ccggcaagcg catcgcggtg
     6841 atcggcgatc tcatcgccga cgagtacctc tacggcaagc ccgcgcgtat atcgcgcgag
     6901 gcgccagtcc tgatcctgcg cttcaccgac cgcgaggtac ggctgggcgg cgcggccaac
     6961 gccgcccaca acgtgcacgc cctcggcgcc accgtcctgc cggtgggcct gctcggccag
     7021 gacggggcgg gcgccgaggt gcacgcgctc ttccaccagg cgggcatgag cacggacggc
     7081 atctcgcgcg agccggcacg acccacgccg gtgaagaccc gcatcatggc gggcggctac
     7141 gagtccacgc ggcaacaggt cgtgcggctc gaccgcgagc cggatggcgg catcagcggc
     7201 cccgcggagg cgcggctgat cgagcgactc caggcggtcg gcgccgacgc cgacgccttc
     7261 ctcgtctcgg actacggcta cggcacggtg accccgcgcg tgtacgagac ggtactcgag
     7321 ctcggccggg cgcggcaggt gccggtgacg gtggacagcc gctacgagct gccccgcttc
     7381 aagggcgcca ccgcggccac gcccaacgag ccggaggtgg aggagctcac cggccaggag
     7441 ctgggggacg agccgtccgt cgagaaggcg gggcgcgcgg tgctcgagcg gctcgacgcg
     7501 cgcctgctcc tcgtcacccg gggcagccgc ggcatggccc tcttcgagcg cgaagggccg
     7561 gtcacgttca tgccgatcca cggcagc
//
LOCUS       DASUFA010000101         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1157389, whole genome shotgun sequence.
ACCESSION   DASUFA010000101 DASUFA010000000
VERSION     DASUFA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157389"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFX87_03805"
     CDS             <1..1140
                     /locus_tag="VFX87_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994064.1"
                     /translation="SVQGLGEIVAFQQEAQRGEALAARATAYARARMPFLADLARQSA
                     LQEVATGLGGLAVTLSGAWLVAGGRLEAAMLPLLTLLAMSAFVPVWEIAQVGRQLADT
                     LAATRRVHAVHGEPVRIADGPGAPGPRREAAAAIALHHVTFTYPGRRQPALQDVSFTV
                     PAGAMVALVGPSGAGKTTIASLLLRFWDPDSGAITLGGHDLRRWRLEGLRREIALVAQ
                     DTHLFNDTLAGNIRIARPEATQAEVAAAIERAALLPLVRSLPEGLDTRVGERGLQLSG
                     GQRQRVAIARAFLREAPVLILDEATSHLDAVNEQVVQEALAGLTHARTTIVIAHRLST
                     VRAADQIVVLDEGRVVEQGRHEDLLRRRGLYARLVSRQVAAAIAS"
     gene            1263..1904
                     /locus_tag="VFX87_03810"
     CDS             1263..1904
                     /locus_tag="VFX87_03810"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX4994065.1"
                     /translation="MKARNALTGLALVLGMALPAGAQTLDGMQLAQAPTDATRPAPGP
                     GAHPHWGQPPEGHGGQHGHHGWQRRARAHRFSLSGLALRHQKDLALTPAQVDSLRKLG
                     TDAQRDAIKRQADQRLAELDLRTMMAPDPGDPNKPRDLARIETKVREIEKLRADGRVA
                     QIRSLEQSRQVLTPEQREKLRTLLSQRMQHRGPHGPGMRGMTPEDGAQKPPAG"
     gene            complement(1964..2860)
                     /locus_tag="VFX87_03815"
     CDS             complement(1964..2860)
                     /locus_tag="VFX87_03815"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4994066.1"
                     /translation="MATITSPLTITPVHPAFGAVVSGVDLTQPLDAAAFERLAVAFDD
                     YSVLVFRGQALSDAQQMAFSERWGPLETTVRTFGGEDRLGANIVDLSNTDADGKLMGW
                     DDRRMLYQSGNQLWHSDSSFKPVPAHSSALSARVVPPEGGETEFASMRVAYETLDENV
                     RRDLDRLIVVHSFGFSRSLIDPGIGTEIGRDYPPVRHALVRANPRNGRRSLYIGSHAW
                     YIEGMGLDESRMLIAKLLEHTVRADRVYRHRWQVGDLVMWDNRCALHRGRLWDSARYK
                     RVMHRTTVAGDGPTADPAYLNA"
     gene            complement(2883..>3498)
                     /locus_tag="VFX87_03820"
     CDS             complement(2883..>3498)
                     /locus_tag="VFX87_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4994067.1"
                     /translation="SLITALVVTAITTVVSSLGGYAVARLRFPGRESVASLILILYLI
                     PPALLFIPLYRVLAELGATNSLSSLFLSYPTFTVPFCTWLLIGFFKALPDELEEAALI
                     DGAGRVTAFVRVLLPLAAPGLVASAIFAFTLSWNEFLYALVFIQDETATTVPVGLNLL
                     IYGDVFHWGQLMAASVITTIPVVALYVFIHRWMVEGLVAGSVKG"
BASE COUNT          548 a         1220 c         1267 g          463 t
ORIGIN      
        1 tccgtgcagg ggctcggcga gatcgtcgcg ttccagcagg aggcgcagcg cggtgaggcg
       61 ctcgccgcgc gggccacggc ctacgcgcgc gcacgcatgc cctttctcgc cgacctcgcg
      121 cgccagtcgg cgctccagga agtggccacc gggctgggcg gcctcgcggt gacgctgtcg
      181 ggcgcgtggc tcgtggcggg gggccggctc gaagccgcga tgctgccgct gctcacgctg
      241 ctcgccatgt ccgccttcgt gccggtctgg gagatcgcgc aggtcggccg ccagctcgcc
      301 gacacgctcg cggccacccg gcgcgtccac gcggtgcacg gcgagccggt gcgcatcgcc
      361 gacgggccgg gcgcccccgg accgcgccgc gaggcggcgg ccgccatcgc gctccaccac
      421 gtcaccttca cctacccggg ccggcggcag cccgcgctgc aggacgtgtc cttcacggtg
      481 cccgcgggcg cgatggtcgc gctggtcggc ccgtccggtg cgggcaagac cacgatcgcg
      541 agcctgctgc tgcgcttctg ggacccggac tcgggcgcca tcacactggg cggccacgac
      601 ctgcggcggt ggcgcctcga ggggctgcgc cgcgagatcg cgctggtggc ccaggacacg
      661 catctcttca acgacaccct ggccggaaat atccgcatcg cgcggccgga ggccacccaa
      721 gcggaggtag cggccgcgat cgagcgcgcc gcgctgctgc cgctggtgcg ctccctgccc
      781 gaggggctcg acacgcgggt gggcgagcgg gggctccagc tctcgggcgg ccagcgccag
      841 cgggtggcca tcgcgcgggc cttcctgcgg gaagcgcccg tcctgatcct cgacgaggcc
      901 acctcgcacc tcgacgcggt caacgagcag gtggtgcagg aggccctggc cggccttacc
      961 cacgcgcgca ccaccatcgt gatcgcccac cgtctctcca cggtccgtgc cgccgaccag
     1021 atcgtggtgc tggacgaggg ccgcgtggtc gagcagggcc gccacgagga tctcctgcgc
     1081 cggcggggcc tttacgcccg gctggtctcc cgccaggtcg ccgccgccat cgcctcctga
     1141 ccttttcctc cccctgtcaa cgatcggggt cgatggttcg tcataggggc agaggcagag
     1201 aacccagcgg ggcccccggt tagcgacgga tccccgctat cgacggatca aggaggatcc
     1261 ccatgaaagc acgcaacgca ctgacaggac tggcactagt tctgggaatg gccctgcccg
     1321 cgggcgccca gaccctggac ggcatgcagc tcgcgcaggc gcccacggat gccacgcgcc
     1381 ccgcgccggg tccgggagcg cacccgcact ggggccagcc gccggaggga cacggcggcc
     1441 agcacggcca tcacgggtgg cagcggcggg ccagggctca tcgcttctcg ctgagcggcc
     1501 tggccctgcg gcaccagaag gacctcgcgc tgacgccggc ccaggtggac agcctgcgca
     1561 agctcgggac ggacgcgcag cgtgacgcca tcaagcggca ggccgatcag cggctggccg
     1621 agctggatct gcgcacgatg atggcgccgg acccgggcga tcccaacaag ccgcgggacc
     1681 tggcccggat cgagaccaag gtgcgggaga tcgagaagct gcgcgcggat gggcgcgtgg
     1741 cccagatcag gagcctggag cagagccgcc aggtgctgac gccggagcag cgtgagaagc
     1801 tgcggaccct cctgagccag cgcatgcagc accgcggccc gcacggcccc gggatgcgcg
     1861 gcatgacgcc cgaggacggc gcccagaagc cccccgccgg ttagctacaa gagcggggtc
     1921 aggtcttaca ttccgacaat gtgggaatgt aagacctgac cccttacgcg ttgaggtagg
     1981 cggggtccgc ggtggggccg tcgccggcca ccgtggtgcg gtgcatgacg cgcttgtagc
     2041 gcgcgctgtc ccagaggcgg ccgcggtgca gcgcgcagcg gttgtcccac atcacgagat
     2101 ctcccacctg ccagcggtgc cggtagacgc ggtcggcccg caccgtgtgc tcgagcagct
     2161 tggcgatgag catgcggctc tcgtcgagcc ccatgccttc gatgtaccag gcgtgcgagc
     2221 cgatgtagag cgaccggcgg ccgttacgcg ggttggcgcg caccagggcg tgccgcaccg
     2281 gcgggtagtc acgcccgatc tcggtcccga tacccggatc gatcaggctg cgggagaagc
     2341 cgaagctgtg cacgactatt agccgatcca agtcgcggcg tacgttctcg tcgagagttt
     2401 cgtaggccac ccgcatgctc gcgaactcgg tctcgccgcc ttcgggcggc accacgcgag
     2461 cggagagcgc ggaggagtgg gcgggcaccg gcttgaacga gctgtcgctg tgccagagct
     2521 ggttcccgga ctggtagagc atccgccggt cgtcccagcc catcagcttg ccgtccgcgt
     2581 cggtattgga gaggtccacg atgttggcgc ccagccggtc ctcgcctccg aacgtgcgca
     2641 cggtggtctc gagcgggccc cagcgctcgc tgaacgccat ctgctgcgcg tcgctcagcg
     2701 cctgtccccg gaagaccagc accgagtagt cgtcgaaggc aaccgcgagg cgctcgaagg
     2761 cggcggcgtc gagcggctgc gtcaggtcca ctccggagac cacggctccg aaggcgggat
     2821 ggacgggcgt gatggtgagc ggactcgtga tggtggccat ggggacctcc tcgcccgaaa
     2881 ttctagccct tcaccgagcc cgcgaccagc ccctccacca tccagcggtg gatgaacacg
     2941 tagagggcga ccaccgggat cgtggtgatg acggaggcgg ccatgagctg cccccagtgg
     3001 aagacgtcgc cgtagatgag caggttgagg cccaccggca ccgtggtggc cgtctcgtcc
     3061 tggatgaaga ccagcgcgta gaggaactcg ttccaggaca gcgtgaacgc gaagatggcc
     3121 gaggccacca gccccggggc ggccagaggc aacagcacgc ggacgaacgc ggtgacccgg
     3181 cccgccccgt cgatcagggc tgcttcctcc agctcgtcgg gcagcgcctt gaagaagccg
     3241 atcagcagcc aagtgcagaa cggcaccgtg aaggtcgggt aggagaggaa caacgacgag
     3301 aggctattcg tcgcccccag ctcggcgagg acacggtaga gcgggatgaa gagcagcgcg
     3361 ggcgggatca ggtagaggat caggatcagg ctcgcgaccg actcgcggcc ggggaagcgc
     3421 agccgcgcca ccgcgtagcc tccgaggctc gagaccaccg tggtgatggc ggtcaccacc
     3481 agcgcggtga tgaggcta
//
LOCUS       DASUFA010000102         7534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_482267, whole genome shotgun sequence.
ACCESSION   DASUFA010000102 DASUFA010000000
VERSION     DASUFA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482267"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFX87_03825"
     CDS             complement(<1..288)
                     /locus_tag="VFX87_03825"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994068.1"
                     /translation="MRAYLLQRLAAMGLTLFGVSLLVFAMVRLIPGTVVEQLLGQASM
                     ASPEVVASFRRFFGLDQPWHVQYRVWLAGLLRGDLGVSWLSGRPVLALFLER"
     gene            complement(310..1881)
                     /locus_tag="VFX87_03830"
     CDS             complement(310..1881)
                     /locus_tag="VFX87_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994069.1"
                     /translation="MDHHGSKNGNRAPIGRRRLLKTGGAALTLAAAGLPRRAAAQGKT
                     GGTLTFASTALPPSLEPHMQGLDIWQRRKPLLYENLVWIDHALEPKPELAERWEQKST
                     TEYVFHLRRGVRFHNGKEMDAEDVKYTYERVRDPKVSPGANDLAYVSQIDAVDKYTVR
                     FVLSAPAATFLANLGGKYNGVIPKDAGGDGKALLTTAVGTGPFALESFDPSRRLALKR
                     HAQYWGAQKPLLDAIVFQAIPDESSIVAGLRTGQITLAEFSSALSFQVARSVPTLEAI
                     QAPSTRWVVLDLAGDQEPTSKLEVRQAIALAIDRAAILQIAGSGLGQRLGVLPPGLKG
                     WALPWQELPNQQRDVAKAKQLLQKAGYTGRVPMKIRNIVGFPALAAALPVIVDNLKEA
                     GIDVTVETVDGGVWIKDWIVPQSPPTMNDWGGYVDPDQAFHRHFHSAPGGRDFRRWKN
                     KAADELMDSGRVTLDRAKRKQLYDQLQRMFAEDPITIPLYSPDLLYVRQKSLKGFQPH
                     STGFYYGLRFASLES"
     gene            complement(1897..2880)
                     /locus_tag="VFX87_03835"
     CDS             complement(1897..2880)
                     /locus_tag="VFX87_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX4994070.1"
                     /translation="MAGTNALTYQVVEGWEQLPKGYEHRDVAGVAVDAEDRVFLICRG
                     DHPIMVYDHKGNFLSSWGEGQFTYRTHGITIGPDGSVYCTDDGNHTVRKFTPQGKLLL
                     TLGTMNKPSDTGYDGKDTMTVAHPGGAFNRPTNLAVGPKGDLYVSDGYGNCRVHQFSP
                     SGELRRSWGVPGKGPGQFYLPHGIAAAADGRIFVCDRENDRIQIFSPDGEYLSEWTDT
                     QRPTHLVFDPQGRAYVSELWWHKGQTSQRHGPITDPRYGRVSVFDRDGRLLTRWGTPD
                     QAAPGSFAAPHGIAVDSSRNIFVAEVTWTFAVSRGLVESGCHTFQKFALTA"
     gene            complement(2965..3504)
                     /locus_tag="VFX87_03840"
     CDS             complement(2965..3504)
                     /locus_tag="VFX87_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEX4994071.1"
                     /translation="MPGVSGAGRSPGVTALDILDNVIPYIPGEEEKVAREPGKILGHL
                     DGSGGIAPARFPVSATCTRVAVSEGHTEAVTVSLGAAAAPADVAVAFDEFGRELTRLA
                     LPSAPHRLITVHDDPFRPQPRLDRDADGGMTTSVGRIREDAALEHEVTYVLVSHNTRM
                     GAAKGAVLTAEYLAVTGHV"
     gene            3730..4584
                     /locus_tag="VFX87_03845"
     CDS             3730..4584
                     /locus_tag="VFX87_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX4994072.1"
                     /translation="MLIAQITDLHISTPGSVNDRHFRTAEHLARAVAHLNGLSPRPDV
                     VLATGDLVERGEPDEYARLRAILDGLAMPLYVIPGNHDTRASLARAFADRGYLPQDGG
                     FLHYTVEDWPVRLIGLDTHVPGASGGRLCAERLDWLDARLGEGRERPTVVFMHHPPFV
                     TGMRAMDAMGLEGKAELAEVIGRHAQVEGVLSGHVHRPMTRRFAGTVATTCPATAHQL
                     ALDLASEPRLAVVMEPPACMLHLWLGTAEGLVSHVSVIGGERPPFTIHDGQRWLRDAV
                     PPPSFHPR"
     gene            4666..5463
                     /locus_tag="VFX87_03850"
     CDS             4666..5463
                     /locus_tag="VFX87_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994073.1"
                     /translation="MTPTASALAWTTEEIRVAGSSVRLRTAGRGAPLLVLHHDIGTLE
                     QLPFYEALADRFTLLLPSHPGYDGSERPAFMRNVRDVAVTYQALLAQRGLADVALVGL
                     GFGGWIAAEMATMAPRAFRKLALVGAMGIKPADGEILDQALVSYIDYVRAGFEDQRAF
                     DRLFTPDPPTPQLEQWDLNREMTFRIAWKPYMYNPTLAPLLGGVQTETLIVWGRQDRI
                     VPVGVGEQYARALPKSRLSVVEGAGHFLEMEQPEALAKLIVPFAAQA"
     gene            5487..6710
                     /locus_tag="VFX87_03855"
     CDS             5487..6710
                     /locus_tag="VFX87_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994074.1"
                     /translation="MYFTEQPMSAYPADEGRKRGATALMFSNRHFDPVAGSRLYNEYL
                     EHYRLAEEVGFDGIMLNEHHNAPFCMQAKCNVFASILAAVTKRVKIVLLGNPLPLADN
                     PVRLAEELAMIDMVSRGRLVSGFVRGGGQEQLATGVNPAFNRERFEEAHELIVRAWTQ
                     EGPFRFEGNHYQHRVVNPWAVPLQKPHPRIWIPGVLSKETIIWAARQRYPYIALSTAI
                     DATKKIWELYDSVAAEAGYTGGPENRGYLQRCHVAETEEKALANAREFMWMQGEFTGL
                     AHPVWSSPSGYFSPSYRKAFVEYAVGRGSNPRAASFEDQLRDQQIIAGTPKTVLPKFR
                     RLLEETRPSILGLWASDGFVSNQDATTCIRLLGQEVLPAVREMGKELGLWSPFEANAP
                     VGINQAAPPAHRNGA"
     gene            complement(6742..6984)
                     /locus_tag="VFX87_03860"
     CDS             complement(6742..6984)
                     /locus_tag="VFX87_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994075.1"
                     /translation="MKRHILTLAWILWAHETAQVGERLIDRGYTAIDSFETRQLCHAA
                     MTDYAGLKLVRQGKVRVEFSCLPEPTSPKPSRSSVG"
     gene            7254..>7534
                     /locus_tag="VFX87_03865"
     CDS             7254..>7534
                     /locus_tag="VFX87_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994076.1"
                     /translation="MATKIIVSPKFPPAMLDIARSLVPAGFEMVVVDAGTPEFTAALP
                     DASYYVGFPRAELNTEFFKAASGLKLVQLISAGYDRLDVAAAKRAGVPVA"
BASE COUNT         1097 a         2626 c         2672 g         1139 t
ORIGIN      
        1 ccgctcgagg aagagcgcca gcaccgggcg gccggagagc cacgagacgc cgagatcgcc
       61 gcgcagcagt ccggccagcc agacgcggta ctggacgtgc cagggctggt cgagaccgaa
      121 gaagcggcgg aacgaggcca ccacctcggg ggacgccatc gaggcctggc ccaggagctg
      181 ctccaccacc gtgcccggga tcagccgcac catcgcgaag accagcaggc tcaccccgaa
      241 gagggtgagc cccatcgccg cgagacgctg caggaggtag gcgcgcacgc gcccgcggga
      301 ccgcgctcct cagctctcga gggaagcgaa gcgcaggccg tagtagaagc cggtcgagtg
      361 cggctggaag cccttgaggc tcttctgccg gacgtagagg agatccggcg agtagagcgg
      421 gatcgtgatc gggtcctcgg cgaacatgcg ctggagctgg tcgtagagct gcttgcgctt
      481 ggcgcggtcg agcgtgacgc ggccgctgtc catcagctcg tcggcggcct tgttcttcca
      541 gcggcggaag tcgcggccgc ccggagcgga atggaagtgg cggtggaagg cctggtcggg
      601 gtccacgtag ccgccccagt cgttcatggt gggcggcgac tgcggcacga tccagtcctt
      661 gatccagacg cccccgtcca ccgtctccac cgtcacgtcg atgcccgctt ctttcaggtt
      721 gtccacgatg accggcaggg cggcggccag ggcggggaag ccgacgatgt tgcggatctt
      781 catgggcacg cgcccggtat agcccgcctt ctgcaggagc tgcttggcct ttgccacgtc
      841 gcgctgctgg ttcggcagct cctgccacgg cagcgcccag cccttgaggc cgggcggcag
      901 cacgccgagc cgctggccga gccccgagcc cgcgatctgg agaatggccg cccggtcgat
      961 ggccagcgcg atggcctggc gcacctcgag cttgctggtg ggctcctggt cgcccgccag
     1021 atcgagcacc acccagcggg tggacggcgc ctggatggcc tcgagcgtcg gtacgctcct
     1081 ggccacctgg aacgacagcg ccgaggagaa ctcggccagc gtgatctggc ccgtccgcag
     1141 accggccacg atcgaggact cgtccggaat cgcctggaac acgatggcgt cgagcagggg
     1201 cttctgggcg ccccagtact gggcatgccg cttgagcgcg agccggcggc tcgggtcgaa
     1261 gctctccagc gcgaagggcc cggtgccgac cgcggtggtc agcagcgcct tgccgtcgcc
     1321 gcccgcgtcc ttcgggatga cgccgttgta cttgcccccc agattggcca ggaaggtggc
     1381 cgcgggcgcg gagagcacga accgcaccgt gtacttgtcc accgcatcga tctggctcac
     1441 gtaggccagg tcgttggcgc ccgggctcac cttggggtcg cggacacgct cgtaggtgta
     1501 cttgacgtcc tcggcgtcca tctccttgcc gttgtggaac cgcacgcccc ggcgcaggtg
     1561 gaagacgtat tcggtggtcg acttctgctc ccagcgctcg gcgagctccg gcttgggctc
     1621 gagcgcgtga tcgatccaga ccaggttctc gtagaggagc ggcttgcggc gctgccagat
     1681 atcgagcccc tgcatgtgcg gctcgaggct cggcggcagc gcggtgctcg cgaacgtgag
     1741 cgtcccgccc gtcttgccct gcgccgccgc gcggcgcggc aggcccgcgg cggcgagagt
     1801 cagcgccgcg ccgccggtct tgagcaggcg ccgccggccg atcggggcgc gattgccatt
     1861 cttcgagccg tgatgatcca tcggacggga gcctcctcag gccgtgagcg cgaacttctg
     1921 gaaggtgtgg cagccgctct cgacgaggcc gcggctcacc gcgaaggtcc aggtgacctc
     1981 cgccacgaag atgttccggc tcgaatccac cgcgatgccg tggggcgccg cgaagctgcc
     2041 gggcgcggcc tgatcggggg tgccccagcg ggtgagcagg cggccgtcgc ggtcgaacac
     2101 gctcacgcgc ccgtagcgcg ggtcggtgat ggggccgtgg cgctgcgagg tctggccctt
     2161 gtgccaccag agctcggaga cgtaggcgcg cccctgcggg tcgaacacca gatgggtcgg
     2221 ccgctgcgtg tcggtccact ccgagaggta ctcgccgtcg gggctgaaga tctggatgcg
     2281 atcgttctcc cggtcgcaga cgaagatgcg cccgtccgcg gcggccgcga tgccgtgggg
     2341 caggtagaac tggccggggc ccttgccggg cacgccccac gagcgccgga gctccccgct
     2401 cggcgagaac tggtgcacgc ggcagttgcc gtagccgtcg gagacgtaga gatcgccctt
     2461 gggtcccacc gccaggttgg tgggccggtt gaacgctccg ccggggtgcg ccaccgtcat
     2521 cgtgtccttg ccgtcgtagc cggtgtccga cggcttgttc atggtgccga gcgtcagcag
     2581 cagcttgccc tgcggggtga acttccggac ggtgtggttg ccgtcgtcgg tgcagtacac
     2641 gctgccgtcc gggccgatgg tgatgccgtg ggtgcggtag gtgaactggc cctcgcccca
     2701 cgaggagagg aagttgccct tgtgatcgta gaccatgatc gggtggtcgc cgcggcagat
     2761 caggaagacg cggtcctcgg cgtccaccgc cacgccggcc acgtcgcgat gctcgtagcc
     2821 cttgggtagc tgctcccagc cctcgacgac ctggtacgtc agcgcgttcg tccccgccat
     2881 ggctcgcccc cccgatggat gcacgtcgat ggaatcggcc ccgtcgcgtc aaactatggg
     2941 agaaaggtcc gaccgagtca agaactagac atggccggtg actgccaggt attcggcggt
     3001 caagacggcg cctttcgccg cgcccatccg cgtgttgtgg gacacgagca cgtacgtgac
     3061 ctcgtgctcc agcgcggcgt cctcgcggat gcggccgacg ctcgtggtca tgccgccgtc
     3121 cgcgtcgcgg tcgagccggg gctgcggccg gaacggatcg tcgtgcacgg tgatcagccg
     3181 gtggggcgcc gacggcagcg cgagccgcgt gagctcgcgg ccgaactcat cgaaggcgac
     3241 ggccacgtcc gcgggcgccg cggccgcgcc gagcgacacg gtgaccgcct cggtatggcc
     3301 ctcgctcacc gcaacccggg tgcaggtggc gctcaccggg aaccgcgcgg gggcgatacc
     3361 ccccgatcca tccaggtggc cgaggatctt gccgggctcg cgcgcgacct tctcctcctc
     3421 gcccgggatg taggggatca cgttgtccag gatgtcgagc gcggtcactc ccggcgagcg
     3481 tcccgcgccc gagacccccg gcaaaacgca cgaggcccgc gatcaccggg tggtggtcgc
     3541 gggcctcgtg gatgcgagga cgtcttcgag tgtctcttag gtctgtcgcc gggcgcgcgc
     3601 cgccttcccc gctgggcgtc ccgtgctccg ggaccccagc cgggtcttgt cgctgctgcg
     3661 cttggcccgg cggctcatag cggtacttgt agtccggagg aggccgacct gtcaatgtat
     3721 gatcaggcca tgctcatcgc ccagatcacc gatctacaca tcagcacgcc cggcagcgtg
     3781 aacgatcggc acttccgtac cgcggagcat ctggcgcgcg cggtggccca tctgaacgga
     3841 ctctcgccgc ggccggacgt cgtcctggcg accggcgatc tcgtcgagcg cggcgagccg
     3901 gacgagtacg cgcgcctgcg cgcgatcctg gacggcctcg cgatgccgct ctacgtgatc
     3961 ccgggcaatc acgacacgcg ggcgtccctc gcgcgcgcct tcgccgatcg gggctacctg
     4021 ccgcaggacg gcggcttcct gcactacacc gtcgaggact ggccggtgcg cctcatcgga
     4081 ctcgacaccc acgtccccgg cgcgtccggc ggccgcctgt gcgccgagcg gctcgactgg
     4141 ctcgacgcgc ggctgggcga ggggagggag cggcccacgg tcgtcttcat gcaccacccg
     4201 ccgttcgtca ccggcatgcg ggcgatggac gccatgggcc tcgagggcaa ggcggagctg
     4261 gccgaggtga tcggccgcca tgcgcaggtc gagggggtgc tgagcgggca cgtgcatcgc
     4321 ccgatgacgc ggcggttcgc gggcacggtg gccaccacct gtcccgcgac cgcgcatcag
     4381 ctcgcgctcg acctggcctc cgagccgcgc ctggccgtgg tgatggagcc gccggcctgc
     4441 atgctgcacc tctggctcgg caccgcggag ggcctcgtca gtcacgtgag cgtgatcggc
     4501 ggcgagcggc ccccgttcac gattcacgac gggcagcgct ggctgcgcga cgcggtcccg
     4561 ccgccctcgt ttcatccccg ctgaccgcgc cgacgacgct cgggctcgtc atcgatccgg
     4621 gcacgcgggt tgcggggcgg aaagcgattt gatagcgtcg ctgccatgac cccgacagcc
     4681 tccgcgctcg cgtggaccac cgaggagatc cgggtcgccg gctcctccgt aagactacgt
     4741 accgccggcc gtggcgcccc gctgctcgtg ctgcatcacg atatcggcac gctggagcag
     4801 cttccattct acgaggcgct ggccgatcgc ttcacgctgc tgctgccctc gcacccgggc
     4861 tacgacggct cggagcggcc ggccttcatg cgtaacgtcc gggacgtcgc cgtgacctac
     4921 caggccctgc tcgcgcagcg cggcctggcg gacgtggccc tggtgggcct gggcttcggc
     4981 ggctggatcg cggccgagat ggccaccatg gcgccgcgcg ccttccggaa gctggcgctc
     5041 gtcggcgcga tgggcatcaa gcccgcggac ggcgagatcc tcgaccaggc cctcgtcagc
     5101 tacatcgact acgtgcgggc gggcttcgag gaccagcggg ccttcgaccg gctcttcacg
     5161 cccgacccgc ccacgccgca gctcgagcag tgggatctca accgcgagat gacgttccgc
     5221 atcgcgtgga agccttacat gtacaacccg acgctggcgc cgctgctggg cggggtgcag
     5281 acggagaccc tgatcgtgtg ggggcggcag gaccggatcg tgccggtcgg ggtgggggag
     5341 cagtacgccc gcgcgctgcc caagagccgg ctcagcgtcg tggagggcgc cggccacttc
     5401 ctcgagatgg agcagcccga agcactcgcg aagctcatcg tgccgttcgc cgcccaggcc
     5461 tgatcggggg atccccaatg cacctcatgt acttcacgga acagccgatg tcggcgtacc
     5521 cggcggacga agggcgcaag cggggcgcca ccgcgctgat gttctccaac cgccacttcg
     5581 atccggtggc gggcagccgg ctctacaacg agtacctcga gcactaccgg ctcgccgagg
     5641 aagtgggctt cgacggcatc atgctgaacg agcaccacaa cgcgcccttc tgcatgcagg
     5701 ccaagtgcaa cgtcttcgcc tcgatcctcg ccgcggtgac caagcgcgtg aagatcgtgc
     5761 tgctcggcaa cccgctgccg ctcgccgaca atccggtgcg gctggccgag gagctggcca
     5821 tgatcgacat ggtctcgcgc gggcggctcg tctccggctt cgtgcgcggg ggcgggcagg
     5881 agcagctcgc caccggggtg aacccggcct tcaaccgcga gcgcttcgag gaggcccacg
     5941 agctgatcgt gcgggcgtgg acgcaggagg gcccgttccg cttcgagggc aaccactacc
     6001 agcaccgcgt ggtgaacccg tgggcggtgc cgctgcagaa gccgcacccg cgcatctgga
     6061 tccccggcgt gctcagcaag gagacgatca tctgggccgc gcgccagcgc tatccctaca
     6121 tcgcgctgag caccgcgatc gacgccacca agaagatctg ggagctctac gactcggtcg
     6181 ccgcggaggc cggctacacc ggcgggccgg agaaccgcgg gtatctccag cgctgccacg
     6241 tcgccgagac cgaggagaag gcgctcgcca acgcgcggga gttcatgtgg atgcagggcg
     6301 agttcaccgg cctggcgcac ccggtgtggt cgagcccatc gggctacttc tcgccgtcgt
     6361 accgcaaggc cttcgtggaa tacgcggtgg gccgcggctc gaatccccgg gccgccagct
     6421 tcgaggacca gctccgcgat cagcagatca tcgcgggcac gccgaagacg gtgctcccca
     6481 agttccggcg cctcctcgag gagacgcggc cgtccatcct ggggctgtgg gcgagcgacg
     6541 gcttcgtgag caaccaggac gccaccacgt gcatccggct gctcgggcag gaagtgctcc
     6601 cggcggtgcg cgagatgggc aaggagctcg ggctctggag cccgttcgag gccaatgctc
     6661 cggtgggcat caaccaggcg gccccgccgg cgcaccgcaa cggggcctga gtgcgatcgg
     6721 gcgacgcgcg cgcgtcccgc gctagccgac cgacgaccgg gacggcttcg ggctggtcgg
     6781 ctcgggcagg cagctgaact ccacgcggac cttgccctgc cggaccagct tgagccctgc
     6841 gtagtccgtc atggccgcgt ggcagagctg gcgcgtctcg aacgaatcga tcgcggtgta
     6901 gccgcggtcg atgagccgct cgcctacctg cgcggtctcg tgggcccaga ggatccaggc
     6961 cagggtcagg atgtggcgct tcatcgggtg gtcgtccccc tgtcgtcggt gccttctcca
     7021 ggagagcaag ggtcggacca taggaaaatc cccatgactt cagcatcttg tatgagaacg
     7081 gctcagacga cagagaaagc gcggtgcgag gctgggaatg ggagtaccca gcctggaggc
     7141 cgccggcccc gcgcaggaga tgccggccgt gcgcgctccc ccgtggtgac cgcccaggcc
     7201 gtatactggc gcgcgtgacc gccccgctca ccccccagcc aaaggaggct cccgtggcca
     7261 ccaagatcat cgtctctccc aagttccccc ccgcgatgct cgacatcgcc cggagcctcg
     7321 tacccgccgg cttcgagatg gtcgtggtgg acgcgggcac gcccgagttc accgccgccc
     7381 tgccggacgc ctcctactac gtgggcttcc cgcgtgccga gctcaacacc gagttcttca
     7441 aggcggcctc gggcctcaag ctggtgcagc tcatcagcgc cggctacgac cgcctcgacg
     7501 tggcggcggc caagcgggcg ggcgtgccgg tggc
//
LOCUS       DASUFA010000103         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_711294, whole genome shotgun sequence.
ACCESSION   DASUFA010000103 DASUFA010000000
VERSION     DASUFA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711294"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFX87_03870"
     CDS             <1..429
                     /locus_tag="VFX87_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX4994077.1"
                     /translation="AFLAADIFGYTAFNANDTDIGQRIEEISVSLDYICPMVYPSGYH
                     LGIPGVRNPVLNPYEIVKESVRLTRQRSQNPAAQVRPWLQDFKDYAFDKRIFGPGEVR
                     AQIRGSDAGGGSGWMLWNPKNDYTSAALRPKTSAISKSSQ"
     gene            432..1391
                     /locus_tag="VFX87_03875"
     CDS             432..1391
                     /locus_tag="VFX87_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4994078.1"
                     /translation="MRAAISAVLLGITLGVAVLAGAQAPTREPNELGRVMILEYHKID
                     NPEGRWTRTPENFRRDLQRLWERGYRLVALTDYLDGKISLPAGTTPLILTFDDSSPGQ
                     FRYIQRGTDWVIDPECGIGILEAFAREHPGFGRAATFYVLPGANPPNRLFNQPDLATR
                     KLQYLASQGYEIGNHSLWHAELGRYSEAVVREQLAGAQEWVQRHVPGYRFRTLALPMG
                     SYPKELGWAVSGEAKGMTYKHDAILMVAGGAAPSPHARTFDPNRLPRIQAVERDLDYW
                     ITHFDRNPGERYVSDGDAATITVASGSTGKLGAVRAARVIERR"
     gene            complement(1408..>3181)
                     /locus_tag="VFX87_03880"
     CDS             complement(1408..>3181)
                     /locus_tag="VFX87_03880"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEX4994079.1"
                     /translation="QPLDTFARKQFYAPLGMRDTAFDPPAAWRKRIAPTEVVNNHGPL
                     RGVVHDGNSRLLHGVAGHAGVFSTASDLSRFCRMLLAGGQLGGRRYLKEATVRAMFAS
                     RVIGESTRGLGWDISSPYSRTLGAYFPMGSVGHTGFTGTAIWMDPATGVYMILLTNRV
                     HPYGKGDVAELRRRISAAVGTRFAPRDEPSVGTASSRSETTSPAADGTPLLPEGSTLT
                     GLDRLVADDFAPLAGRTVGLITNQTGIDSQGRRAVDLLAAAPQVKLRALFSPEHGITG
                     LVDANVPHGRDAATGLAIWSLYGPARRPSPEQLNGIDTLVFDIQDVGVRYYTYLTTLV
                     YALEEGGRRGIQVMVLDRPNPITGSVVEGPLMDPDMRSFTAPHTIPVRTGMTIGEFAQ
                     MVVAERKLPVKLTVIPLEHWQRNQWFDETGLTWVNPSPNIRNQMQALLYSGVGLLEAT
                     NLSVGRGTDMPFEVIGAPWITDPQVLADAMNARGLAGVLFHPVFFTPTSSVHAGRQVG
                     GVRLEVSDRDALRPVAVGLALGRELMERYPGNFRPAAIQNLLVNRSTMWSVLRGDPFA
                     RILAWADSARNAFLQRRASYLIYK"
BASE COUNT          497 a         1144 c         1100 g          440 t
ORIGIN      
        1 gcgttcctgg ccgccgacat cttcggctac accgccttca acgcgaacga caccgacatc
       61 gggcagcgta tcgaggagat ctcggtgagc ctcgactaca tctgccccat ggtgtaccca
      121 tcgggctacc acctgggcat cccgggcgtc cggaacccgg tgctgaatcc ctacgagatc
      181 gtcaaggaga gcgtccggct gacccgccag cgctcccaga accccgccgc gcaggtgcgg
      241 ccgtggctgc aggacttcaa ggactacgcc ttcgacaagc ggatcttcgg ccccggtgag
      301 gtgcgcgcgc agatccgcgg cagcgacgcc ggcggcggct ccggctggat gctgtggaac
      361 ccgaagaacg actacacgag cgccgcgctc cgtcccaaga cctccgccat ctccaagtcg
      421 tcccagtagg catgcgcgcc gcgatctcgg cggtcctgct cggcatcacc ctgggtgtcg
      481 ccgtactggc gggcgcccag gcgcccacgc gggagcccaa tgagctcggc cgcgtgatga
      541 tcctcgagta ccacaagatc gacaacccgg agggccgctg gacccgcacg ccggagaact
      601 tccggcgcga cctgcagcgc ctctgggagc gcggctaccg cctggtggca ctcaccgact
      661 acctggacgg caagatctcg ctccccgcgg gcaccacgcc gctcatcctg accttcgacg
      721 actcctcgcc gggccagttc cgctacatcc agcgcggcac cgactgggtc atcgaccccg
      781 agtgcgggat cggcatcctg gaagccttcg cgcgcgagca cccgggcttc ggccgcgcgg
      841 ccacgttcta cgtgctgccg ggcgccaacc cgcccaaccg gctcttcaac cagcccgacc
      901 tggccacccg gaagctgcag tacctggcca gccagggcta cgagatcggc aatcactcgc
      961 tctggcacgc ggagctgggc cgctactccg aagcggtcgt acgcgagcag ctcgcgggcg
     1021 cgcaggagtg ggtgcagcgc cacgtgcccg gctatcgctt ccgcaccctc gcgctcccga
     1081 tgggcagcta ccccaaggag ctcggatggg cggtatcggg cgaggccaag ggcatgacgt
     1141 acaagcacga cgccatcctc atggtggcgg gcggcgcggc tccgtccccg cacgcgcgga
     1201 ctttcgaccc caaccgcctg ccccgcatcc aggcggtgga gcgcgacctc gactactgga
     1261 tcacccactt cgatcggaac ccgggtgagc gctacgtgag cgacggtgac gccgcgacga
     1321 tcacggtggc gagcggcagc accggcaagc tgggcgcggt gcgcgccgcc cgcgtgatcg
     1381 agcggcggta gccgcccggg cccggcgcta cttgtagatc aggtaggacg cgcggcgctg
     1441 caggaaggcg ttgcgcgcgg agtcggccca ggcgaggatg cgcgcgaacg ggtcaccccg
     1501 gagcacggac cacatcgtgg accggttcac cagcaggttc tggatggccg ccggccggaa
     1561 gttccccgga tagcgctcca tcagctcgcg acccagcgcg agccccaccg cgaccggccg
     1621 gagcgcgtcg cggtcgctca cctcgagccg caccccgccg acctgccgcc ccgcgtggac
     1681 gctcgaggtc ggcgtgaaga agacgggatg gaacagcacc ccggccaggc cgcgcgcgtt
     1741 catcgcgtcg gccagcacct ggggatccgt gatccagggc gcgccgatca cctcgaaggg
     1801 catatcggtg ccgcgcccca ccgagaggtt ggtcgcctcc agcaggccga cgcccgagta
     1861 cagcagcgcc tgcatctggt tccggatatt gggcgacggg ttcacccacg tgaggccggt
     1921 ctcgtcgaac cactgattgc gctgccagtg ctcgagcggg atcaccgtga gcttgaccgg
     1981 cagcttccgc tccgccacca ccatctgggc gaactccccg atggtcatcc cggtgcgcac
     2041 cgggatggtg tggggcgccg tgaaggagcg catgtcggga tccatcagcg ggccttccac
     2101 gacgctaccc gtgatggggt tcggccggtc cagcaccatg acctggatcc cgcggcggcc
     2161 gccctcctcc agcgcgtaca ccagcgtggt gaggtacgtg tagtagcgca caccgacgtc
     2221 ctggatgtcg aagaccaggg tgtcgatgcc gttgagctgc tcaggcgacg ggcggcgggc
     2281 gggaccgtag aggctccaga tcgccaggcc ggtggccgcg tcgcgcccgt ggggcacgtt
     2341 ggcatccacg agcccggtga tgccgtgctc cggcgagaag agcgcccgga gcttcacctg
     2401 cggggccgcg gccagtagat ccaccgcgcg tcgcccctgg gagtcgatcc cggtctggtt
     2461 cgtgatgagg ccgaccgtgc gcccggccag cggcgcgaag tcgtcggcca ccaggcgatc
     2521 gagcccggtc agcgtggatc cctccggcag gagcggcgtc ccgtccgccg ccgggctggt
     2581 cgtctcgctc cggctcgacg cggtcccgac cgacggctcg tcccggggcg cgaagcgcgt
     2641 gccgaccgcg gcgctgatcc gccgacgcag ctcggccacg tcgcccttgc cgtagggatg
     2701 cacccggttg gtgagcagga tcatgtacac gccggtcgcc ggatccatcc agatggcggt
     2761 gccggtgaag ccggtatggc ccaccgagcc catcgggaaa taggcgccga gggtgcggga
     2821 gtagggcgac gagatgtccc agccgagacc gcgcgtggac tcgccgatca cgcgggacgc
     2881 gaacatggcc cggacggtgg cctccttcag gtagcgtcgg ccgccgagct gcccaccggc
     2941 caggagcatg cggcagaagc ggctcaggtc gctcgcggtc gagaacacgc cggcgtgccc
     3001 cgcgacgccg tgcagcagtc gggaattgcc gtcgtgcacc accccgcgga gcggaccatg
     3061 gttgttcacc acctcggtgg gcgcgatgcg cttgcgccac gccgcgggcg ggtcgaacgc
     3121 ggtgtcgcgc atgccgagcg gggcgtagaa ctgcttccgc gcaaaggtgt cgagcggttg
     3181 a
//
LOCUS       DASUFA010000104         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_826735, whole genome shotgun sequence.
ACCESSION   DASUFA010000104 DASUFA010000000
VERSION     DASUFA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826735"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /gene="tilS"
                     /locus_tag="VFX87_03885"
     CDS             complement(<1..1259)
                     /gene="tilS"
                     /locus_tag="VFX87_03885"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX4994080.1"
                     /translation="MTTATSLVVRVQDTIRRHGMLRGGEAVLVAVSGGADSVALLDVL
                     CAVRESLGLTLTVVHVHHGLRLEADAEADGVRHLCEHLGVACHVERVAVRRGPPWDGL
                     EAESRRARHAALERVARVVGAARIATGHTADDQAETVVMRLLQGAGPRGLGGIAPARG
                     LLIRPLIETRRTTIEEYLRGRGLAWAEDPSNRDVRFLRNRIRHDLLPFMAELTGASVV
                     EALGRSAAAARAVVADLEERARVDLARLASRDGAGISLDVGALAEQPIELAAEMLRQA
                     AAVLGETRPLRGPAQRALRRLLGEAPPRRVVRLGRLAVERSGRRLRVGPVALPALVGR
                     TWAVPGTLALPEIDARLTARVVARARDYAIPRERGRAAFDADALPSALTVRARRRGDV
                     FSPFGAPALRRLKSFLIDAGVPRWQRAR"
     gene            complement(1243..1938)
                     /locus_tag="VFX87_03890"
     CDS             complement(1243..1938)
                     /locus_tag="VFX87_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX4994081.1"
                     /translation="MKVQVRLFARYREEAGRDHLDLELADGGTVERAWEAVTRQYPVL
                     VPYRPYTLFALRNDYVGADHRLGDGDELCLFPPVSGGATGPGQDWIEVTTEPLSERAV
                     AQAVEHPGAGALSLFSGVVRDQTGGRRVKFLEYEAHGPMALAKMREIGAVLRSRWPAI
                     TRIALVHRIGRLEIGESSVMIAVSSPHRAQAFEACRFAIDTLKETVPIWKKEHFEDGE
                     VWVGLQSQCDHRH"
     gene            complement(1967..2293)
                     /locus_tag="VFX87_03895"
     CDS             complement(1967..2293)
                     /locus_tag="VFX87_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX4994082.1"
                     /translation="MVTLTETAAKKISDLRLEEGKPEWGLRIRIVGGGCSGMSYELGW
                     DDTPVEGDNVVETNGVKVFVDSHSAPYLQGSEIDYVDNNMLGAGFAIKNPNVKSSCGC
                     GSSHQF"
     gene            complement(2393..3109)
                     /locus_tag="VFX87_03900"
     CDS             complement(2393..3109)
                     /locus_tag="VFX87_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994083.1"
                     /translation="MKLAGQVAVVTGAGRGIGRAVALRLAGEGAAVTLAARSAPELEG
                     VAREIIQAGGRALAVPTDVRQEAAVEALARRALAEWGRVDALVNAAGLATFAPVTDSK
                     LDDWDQMLAVNLRGAVLCCRAVLPAMVAQRRGTIVNVGSVVTSRALTGSAAYTASKYG
                     LLGFSRVLAEEMRAHGVRVGVLSAGATDTPLWDAMPAAPARERMLRPDQVAEAALLMA
                     SLDSNATLEELTLLPAGGIL"
     gene            complement(3106..3669)
                     /gene="folE"
                     /locus_tag="VFX87_03905"
     CDS             complement(3106..3669)
                     /gene="folE"
                     /locus_tag="VFX87_03905"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX4994084.1"
                     /translation="MIERLIHDLLKEIGEDPTREGLEKTPARVAKAWEYLTSGYKQDV
                     RAVLNEALFTEEYDEMVVVKDIDFYSLCEHHLLPFFGKCHIAYMPSHKIVGLSKLPRL
                     VEMFARRLQVQERLTTQIAQMLNEVLQPRGVAVVIEALHMCMLMRGVEKQNSKAVTSA
                     MLGAFRENAETRAEFMELIRSRGGLVI"
     gene            complement(3666..4100)
                     /locus_tag="VFX87_03910"
     CDS             complement(3666..4100)
                     /locus_tag="VFX87_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX4994085.1"
                     /translation="MSAFTVTRAYHFSAAHQLANPALSDEENARIYGQCFRQHGHNYG
                     LEVTVAGALDRATGMSVDLGVVDAAVKAAVLDRVDHYDLSATVPALAGVITTGENLAR
                     VFYQWLEDALPPDVLRRVTLVETANNVFEYSGETHADEVIPR"
BASE COUNT          612 a         1557 c         1508 g          570 t
ORIGIN      
        1 cgcgcgcgct gccatcgcgg gacgcctgcg tcgatcagga acgatttgag cctccgcagc
       61 gcgggtgcgc cgaacggcga gaagacgtcg ccgcggcgcc gggcgcgcac cgtgagcgcg
      121 gagggaagcg cgtcggcgtc gaaggcggcg cgcccgcgct cgcgcgggat cgcgtagtcg
      181 cgggcgcgcg cgacgacccg ggcggtgagc cgcgcgtcga tctcgggcag ggcgagcgtg
      241 cccggcacgg cccaggtgcg ccccacgagc gcgggcagcg ccaccggacc gacgcgaagc
      301 cgccggccgc tgcgctccac ggccagacgg cccagccgga ccacgcgacg gggcggggcc
      361 tccccgagca gtcggcggag cgcgcgctgg gccggccccc gcagcgggcg cgtctcgccg
      421 aggaccgcgg cggcctggcg gagcatctcg gcggccagct cgatgggctg ctcggcgagc
      481 gcaccgacgt cgaggctgat cccggctccg tcgcggctgg ccaggcgcgc gaggtcgacc
      541 cgcgcccgct cttccagatc ggccaccacc gcacgggccg ccgccgccga gcggccgagg
      601 gcctccacca ccgaggcgcc ggtgagctcc gccatgaagg gcagcaggtc gtggcgaatg
      661 cggttgcgaa ggaagcggac gtcgcggttg ctcggatcct ccgcccaggc gagcccgcgg
      721 ccgcgcaggt actcctcgat cgtggtccgt cgcgtctcga tcagcgggcg gatcaggagc
      781 ccgcgcgccg gcgcgatgcc gcccaggccg cgcgggccgg cgccctgcag caggcgcatc
      841 accacggtct cggcttggtc gtccgcggtg tggccggtgg cgatgcgcgc ggccccgacc
      901 acgcgggcca cgcgctcgag cgcggcgtgc cgggcccggc ggctctccgc ctcgagcccg
      961 tcccagggcg gcccccggcg caccgcgacc cgctccacat ggcaggccac acccagatgc
     1021 tcgcagaggt ggcggacgcc gtcggcctcc gcgtcggcct cgagacgcag gccgtgatgc
     1081 acgtgaacga cggtcagcgt gaggccgagg gactcccgca ccgcgcagag gacgtcgagg
     1141 agggcgaccg agtcggcgcc gcccgagacg gccaccaaga cggcttcgcc gccgcggagc
     1201 atcccgtgac ggcgaatggt gtcctggacg cggacgacga ggctagtggc ggtggtcaca
     1261 ctgcgactgc aatcccaccc acacctcgcc gtcctcgaag tgctccttct tccagatcgg
     1321 caccgtctcc ttcagcgtgt cgatggcgaa gcggcacgcc tcgaaggcct gggcgcgatg
     1381 gggcgaggac accgcgatca tcacgctgga ttcgccgatt tccaggcggc cgatgcggtg
     1441 cacgagcgcg atccgcgtga tggcgggcca gcggctccgc aggactgcgc cgatctcgcg
     1501 catcttggcc agcgccatgg gcccgtgggc ctcgtactcg aggaacttca cccggcgccc
     1561 gccggtctgg tcgcgcacca cgcccgagaa cagggacagc gcccccgcgc ccggatgctc
     1621 caccgcctgg gctaccgcgc gctcggacag aggctcagtc gtgacctcga tccagtcctg
     1681 cccgggcccg gtcgcgccgc cgctcaccgg gggaaacagg cagagctcgt cgccgtcccc
     1741 gagccggtga tcggcgccga cgtagtcgtt gcgcagggcg aagagggtgt aggggcgata
     1801 cggaacgagg acaggatact ggcgggtgac cgcttcccag gcgcgctcga cggtgccgcc
     1861 gtccgcgagc tccagatcca gatgatcgcg gcccgcctcc tcgcggtagc gggcgaacag
     1921 ccggacctgg accttcatcg agcgagcctg ggctctctac gcccgactag aactggtgcg
     1981 acgagccgca gccgcaggac gacttcacgt tcgggttctt gatggcgaac ccggcgccca
     2041 gcatgttgtt gtccacgtag tcgatctcgc tgccctggag atacggcgcg ctgtgggaat
     2101 ccacgaacac cttcacgccg ttcgtctcga cgacgttgtc gccctcgacc ggggtatcgt
     2161 cccacccgag ctcgtacgac atgccggagc agccgccgcc cacgatgcgg atccgcagac
     2221 cccactcggg cttgccttct tcgagccgca gatccgagat cttcttggcg gccgtctcag
     2281 tcaacgtgac cattcgtgcc tccttgggtt tccccgcaga cgcgggggat cacacgctac
     2341 ggcctgatta tagcagaacg gctgggggcc tgccggggcc ccgtggggcg gctcacagga
     2401 tgccacccgc ggggagcagg gtcagctctt ccagggtcgc gttggagtcc agagaggcca
     2461 tcagcagggc ggcttccgcc acctggtccg gacgcagcat gcgctcccgc gccggcgccg
     2521 ccggcatggc gtcccagagc ggggtgtcgg tggcgccggc cgagagcacc ccgacccgca
     2581 ccccgtgggc ccgcatctcc tcggccagca cgcgcgagaa gccgagcagc ccgtacttgg
     2641 acgcggtgta ggcggcgctg ccggtgagcg cccggctggt gaccaccgag cccacgttga
     2701 cgatggtgcc gcgccgctgg gcgaccatgg cgggcaggac ggcccggcag cacagcaccg
     2761 cgccgcggag gttcaccgcc agcatctggt cccagtcgtc cagcttggag tcggtcaccg
     2821 gcgcgaaggt ggcgaggccg gccgcgttca ccagcgcgtc cacccgcccc cactcggcga
     2881 gcgcccgccg cgcgagggcc tccaccgcgg cctcctggcg cacgtcggtg gggacggcca
     2941 gggcccgccc gccggcctgg atgatctcgc gcgccacgcc ttccagctcg ggcgcggaac
     3001 gcgcggccag cgtcaccgcg gccccctcac cggccaggcg cagcgcgacc gcgcggccga
     3061 tgccgcgacc ggctccggtg acgacggcga cctgcccggc gagcttcaaa tgaccagccc
     3121 gccgcgcgac cggatcagct ccatgaactc cgcgcgggtc tccgcgttct cgcggaaggc
     3181 gcccagcatg gcggaggtga ccgccttgga gttctgcttc tccacgcccc gcatcagcat
     3241 gcacatgtgg agcgcttcga tcaccaccgc gaccccgcgc ggctgcagca cctcgttcag
     3301 catctgcgcg atctgggtgg tgagccgctc ctgcacctgc agccggcgcg cgaacatctc
     3361 caccagccgc ggcagcttgg acagcccgac gatcttgtgc gacggcatgt acgcgatgtg
     3421 acacttgccg aaaaacggga ggaggtgatg ctcgcacaac gaatagaagt cgatgtcctt
     3481 caccaccacc atctcgtcgt attcctcggt gaagagcgcc tcgttgagca ccgcgcgcac
     3541 gtcctgcttg tagccggagg tgaggtactc ccacgccttg gccacgcggg ccggcgtctt
     3601 ctccaggccc tcgcgcgtcg ggtcctcgcc gatctccttc aggaggtcgt gaatgagccg
     3661 ctcgatcatc gtgggatcac ctcgtcggcg tgggtctcgc cggagtactc gaacacgttg
     3721 ttggcggtct ccaccagggt cacgcgccgg agcacgtccg gaggcaacgc gtcctcgagc
     3781 cactggtaga acacccgggc caggttctcg ccggtcgtga tcacgcccgc gagcgcgggc
     3841 acggtggcgg agagatcgta gtggtccacg cggtccagca ccgccgcctt caccgcggcg
     3901 tccaccacgc cgagatccac cgacatcccg gtcgcgcggt ccagcgcgcc ggccaccgtc
     3961 acctcgagcc cgtagttgtg cccgtgctgg cggaagcact ggccgtagat ccgcgcgttc
     4021 tcctcgtcgg aaagggcggg gttggcgagc tggtgcgcgg cgctgaagtg atacgcccgg
     4081 gtgaccgtga aggcgctcac ccgccgaggt actccacgta gaaggtggga tcctcccaga
     4141 gccgcaggcg gtgcaggcgc tcggcgccga gcttcgggcc gagcaggtcc cagatcacgc
     4201 ggacgaggtt ctcggtggtg gggatggtcc cggccgggaa gagcggg
//
LOCUS       DASUFA010000105         7585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1392577, whole genome shotgun sequence.
ACCESSION   DASUFA010000105 DASUFA010000000
VERSION     DASUFA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392577"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFX87_03915"
     CDS             <1..217
                     /locus_tag="VFX87_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4994086.1"
                     /translation="VHNLAGSAQPVELDLRAWRGHTPVEMLGDTRFPTIGAGLYFVSL
                     APYGYYWFRLQPPLPDQPLYGIEAAAL"
     gene            192..1643
                     /locus_tag="VFX87_03920"
     CDS             192..1643
                     /locus_tag="VFX87_03920"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994087.1"
                     /translation="MASRPLHSDPAALVGLLSGSGRSDLTRFLIRQRWFAAKTRGIAA
                     VDVLDWAVLDPDGPLVLLLVAVDGDPYYVPVSVAVEAAPEATLARAGAEAVVDAHHDP
                     RFGRRVLAAIASGRSADGRHGRFAFRPTAGWAFPPDPDALAARRLTGEQSNTSVVVGD
                     LVLKSLRRPQPGLNPDLEITRFLTTRTAFRHAPRLAGWVEYAGTGEPATLAVLQEFVP
                     NTGDGWTHVVSTLAGRGATIERRPDPLLDEVGRLGAITGGLHAALASDARDVDFRPEP
                     VGRDDVARWAEEITRELAAPALGARLAGDPEHVTGAVARALEALDRLAGATAKIRVHG
                     DYHLGQVLKTPNGFVIIDFEGEPARPLAERRRKQPALRDVAGMLRSLDYAAHAVAFRR
                     PEGERAAALAALTAWEAEAREAFLAGYLRSVAESPVPLAPATAEALLSACAPFELQKA
                     AYELRYELDNRPDWVAIPQAGIRRILDRSRTSD"
     gene            complement(1606..1797)
                     /locus_tag="VFX87_03925"
     CDS             complement(1606..1797)
                     /locus_tag="VFX87_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994088.1"
                     /translation="MNLLPMAARATIGLTHATAMTLGALVLVTSLVPVFAVCMVALGV
                     REPGRGRLSRSSGSGRESV"
     gene            2068..2217
                     /locus_tag="VFX87_03930"
     CDS             2068..2217
                     /locus_tag="VFX87_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX4994089.1"
                     /translation="MLWTIFVILLVLWAVGMVSSYTLGGFIHLLLLIAIAVILIRVIQ
                     GRRPI"
     gene            2236..2838
                     /locus_tag="VFX87_03935"
     CDS             2236..2838
                     /locus_tag="VFX87_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX4994090.1"
                     /translation="MNYKVQDHLKPKALRGISDDQIAQHWKLYEGYVKSANGLLDGAT
                     KAQAGSPTWSELKRRLGFEIDGVVLHEYYFGNLASGSKLSPGSDLAGDLGATWGSVGA
                     WREDFAKTGGMRGVGWAILYRDPATGGLFNWWVSSHEQGHPAGFNPVVVLDVFEHAFM
                     VDHGAGGREDYIAAFLENVNWEVVEQRHKDSKVGRFTARF"
     gene            2852..3073
                     /locus_tag="VFX87_03940"
     CDS             2852..3073
                     /locus_tag="VFX87_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HEX4994091.1"
                     /translation="MKSGVLIIAIVLIAIGVVSLAYQGITYTTREKILDLGPIKATAD
                     KERTIPLPPILGGLALAGGVVLLVVGARR"
     gene            3275..4693
                     /locus_tag="VFX87_03945"
     CDS             3275..4693
                     /locus_tag="VFX87_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX4994092.1"
                     /translation="MTREVSARAARYRRRGLRPGDRVLLHFGNRLEFFAELLAVWRSG
                     AVAVPVDGTLTRVELERLVETVAPRFSVVDDTTGGAIARALSPGATVVDATEPLPPAD
                     LPEDEARPARDALVLFTSGSSGVPKGVVHTHASLAARWSALRQKLGLVAYARTLCLLP
                     THFGHGLICNSLFPWLAGQDLFITPPFRPDLLLRLGGIIDEHRISFLSSVPAMWRLAL
                     RVARPPRGGTLHRIHCGSAPLPATLWESVRGWAGTPEVLNTYGLTETGSWVAGTSDGP
                     FTPADGLIGPPWGAILHVQRADAMPCAMGEVGEVWIESPALMRGYLGQDELTRRAIQH
                     GRFATGDLGLLDERGWLHLRGRERDEINKGGLKISPAEVDAAAEGCRRVADVCAFAVD
                     DPLYGQNVGLAVALTEPDDAAVREVYGWIRERLAEGKLPAHWWLVDTIPRSERGKVSR
                     DAVREACAGRPPLDLTRILQER"
     gene            4694..4945
                     /locus_tag="VFX87_03950"
     CDS             4694..4945
                     /locus_tag="VFX87_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX4994093.1"
                     /translation="MRHRHRERVLDFLRTVQKPDRPLDTVAETDSLVESGLIDSLALL
                     EIVAFLEEQYGIDFAAVGLEPEQLTSVSGILDLIEREAR"
     gene            4942..6327
                     /locus_tag="VFX87_03955"
     CDS             4942..6327
                     /locus_tag="VFX87_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994094.1"
                     /translation="MTAATMGAAARRLRSFHEDPSVIARWQAWTGPLLTRLTPPRRRA
                     LLALAALWVVIKRPLKELAPAIELRSPNRPTAATAVVLLCLAIVAVMYLVARRFPALP
                     AVVRARPQIWLHGLFWVLVLVCWLVPKGRTVTTLALAGLVLALPFLLWRIGYMLMSGQ
                     RGRIAGTRFSDHLLYLYPAYGGTNTPYGKGIDHLARHEAQTDEALARSQLAGLKLLAL
                     AWIYDRGLDLLAGLIHGQPSGLLGAYGLGLPRLETLMPMGARVPWLTAWAAVYAELVS
                     DVLKLAVRGHEIVGVLRIFGFNVFRNTYKPLLAPSLVEFWNRYHYYFKELLVEFFFFP
                     TYVRRVRKSPRLRMLAATLAAAGFGNFYYHLLQQDAFMLTGSWAALHPWIETRALYCL
                     ILALGIYVSMRREQQRRGTPAAAHAGSAVRLRRIAGVWTFFALIGIWGEAGTASFAQR
                     ADFVLALFGLR"
     gene            6324..>7585
                     /locus_tag="VFX87_03960"
     CDS             6324..>7585
                     /locus_tag="VFX87_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX4994095.1"
                     /translation="MSRTIPGSVRLAAAALGLVALNLVLLAVYRLLFVAWFAPRAAWG
                     EVPAVLLHGLRLDVALLGLELLVIGGLTLLTRHARGHVLVASLWLLTTLNVTVAAVNL
                     LFVRERNQHLWEMLFAYLAEPRDIWVALRPFLWGHPLLVALVILALAGLVMLATRHVR
                     RLGPHRYDLWRPWYVPSLALGALALFALPMAQPITVKQVGGRAKTEVAWIASRHQMAL
                     DDYVLNQAVVNPLWDLVREYLPVAITGGRPPYRLETAEALPLAQRLLGIPEGDRPYPL
                     LRTLRGTGGLGIRNVVLIQVEGLGATLLERDEPQGPVLPFLRSLAAEGLYFPRVYQSF
                     PSTDGAVFATLTSLHWTHALGGSGVRLSQSVMGTYFASLPRLLTAPGAQHYAFSGFRH
                     RTAELTSFQRNLGFRAAGFDALGTRLGP"
BASE COUNT          959 a         2876 c         2572 g         1178 t
ORIGIN      
        1 ggtgcacaac ctcgccggct cggcccagcc ggtggagctc gacctgcgcg cctggcgggg
       61 ccacaccccg gtggagatgc tgggcgacac gcgctttccc accatcggcg cggggcttta
      121 cttcgtcagc ctggcgccct acggctacta ctggttccgg ctgcagcctc ccctgcccga
      181 ccagccgctc tatggcatcg aggccgctgc actctgaccc cgccgcgctc gtcgggctgc
      241 tctcgggttc cggccgctcc gatctcacgc gcttcctgat ccgccagcgc tggttcgcgg
      301 ccaagacccg cggcatcgcg gcggtggacg tgctcgactg ggcggtcctc gaccccgacg
      361 gaccgctcgt gctcctgctc gtcgcggtgg acggcgaccc gtactacgta ccggtcagcg
      421 tggcggtcga ggccgcgccg gaggccacgc tcgcccgcgc cggcgcggag gcggtggtgg
      481 acgcccacca cgacccgcga ttcggccgcc gcgtcctcgc cgccatcgcc tccggccgct
      541 cggccgacgg ccggcacggc cgcttcgcct ttcggcccac ggcgggctgg gccttcccgc
      601 ccgatccgga cgccctggcc gcccggcggc tcaccggcga gcagagcaat acctccgtcg
      661 tggtgggcga tctcgtgctg aagtcgctgc gccggcctca gccgggcctc aacccggatc
      721 tcgagatcac gcggttcctc accacccgga ccgctttccg tcacgcaccg cgactcgcgg
      781 gctgggtgga gtacgccggc acgggcgagc ccgcgacgct cgcggtgctc caggagttcg
      841 tccccaacac cggcgacgga tggacccacg tcgtctcgac gctcgccggc cgcggcgcga
      901 cgatcgagcg acggcccgat ccgctgctgg acgaggtcgg tcgcctgggc gcgatcaccg
      961 gcggcctgca cgccgcgctg gcctccgacg cccgcgacgt ggacttccgc cccgagcccg
     1021 tcggacgcga cgacgtcgcc cgatgggccg aggagatcac gcgcgagctg gccgcgcccg
     1081 ccctcggcgc gcgactcgcg ggcgaccccg agcacgtgac gggcgcggtg gcgcgggcgc
     1141 tcgaggcgct cgatcggctc gcgggcgcga cggcgaagat ccgcgtccac ggcgactacc
     1201 atctcggcca ggtgctcaag acgccgaacg gcttcgtgat catcgacttc gagggcgagc
     1261 cggcccggcc gctcgcggag cgccgccgga agcagccggc cctgcgcgac gtggcgggca
     1321 tgctgcgctc cctggactac gcggcgcacg cggtggcctt ccgtcgcccg gaaggcgagc
     1381 gggcggcggc cctcgccgcc ctcaccgcct gggaggcgga ggctcgcgaa gccttcctcg
     1441 ccggctacct tcgctcggtg gcggagagcc cggtaccgct cgccccggcg accgccgagg
     1501 cgctgctgtc cgcgtgcgcc cccttcgagc tgcagaaggc ggcctacgag ctccgctacg
     1561 agctcgacaa ccgacccgac tgggtcgcga tcccgcaggc cgggatcaga cggattctcg
     1621 accggagccg gacgagcgac tgagtcgccc gcggcccggc tcccgcaccc cgagcgcgac
     1681 catgcacacc gcgaacaccg gcaccaggct cgtcacgagg acgagggcgc ccagcgtcat
     1741 cgccgtggcg tgcgtcaatc cgatcgtcgc tcgggccgcc atcggcagca ggttcaccag
     1801 ctgtgacccc cgtggagttc tcatgacgcc tgaatcgggc aagcagcgtg ccacctggcc
     1861 gtgacggccc gcgatcggga cccttctacc cggtaatcct gacgcacctc ccgggtgccg
     1921 cgcccccgct cgcgccgcgc tccgcgactt ctgggtcggg gaatcgccta cttcggcgac
     1981 gcggtccccc gtggcacgtg gcgtgcgtgt cagagacgcg accgaactcg aaaattccta
     2041 ctcgccgcac acaggaggac gactcacatg ctgtggacga tcttcgtgat cctgctcgtg
     2101 ctctgggcgg tcggcatggt cagttcttac acgctcggcg ggttcatcca tctcttgctg
     2161 ctcatcgcga tcgcggtgat cctgatccgg gtcatccagg gccgacgacc gatatagagc
     2221 cgaggggagc accccatgaa ctacaaggtc caggaccatc tcaagccgaa ggcgctgcgc
     2281 ggcatctccg acgatcagat cgcccagcac tggaagctct acgagggcta cgtcaagagc
     2341 gccaacggcc tcctcgacgg cgccacgaag gcccaggccg gatcaccgac gtggtcggag
     2401 ctcaagcgtc ggctcggctt cgagatcgac ggcgtcgtgc tccacgagta ctacttcggc
     2461 aacctcgcct ccggctccaa gctctccccg gggagcgacc tcgcggggga cctgggcgcg
     2521 acctggggct cggtcggcgc ctggcgcgag gacttcgcca agaccggggg catgcgcggc
     2581 gtcggctggg cgatcctcta ccgggacccg gccaccggag gcctcttcaa ctggtgggta
     2641 tcgagccacg agcagggcca cccggccgga ttcaacccgg tcgtcgtgct cgacgtcttc
     2701 gagcacgcct tcatggtgga ccacggcgcg ggaggccggg aggactacat cgcggccttc
     2761 ctcgagaacg tcaactggga ggtcgtcgag cagcgccaca aggacagcaa agtcgggcgg
     2821 ttcaccgccc gcttctgaga gaggacattg catgaagagc ggagtgctca tcatcgccat
     2881 cgtgctgatc gccatcggcg tggtatccct cgcctaccag gggatcacct acaccacgcg
     2941 ggagaagatc ctggatctcg gtcccatcaa ggcgaccgcc gacaaggagc ggacgatccc
     3001 cctcccgccg atcctgggcg ggctcgcgct ggccggcggg gtcgtgctgc tcgtcgtggg
     3061 agcccgacgg tagcctgata tgtgatccag cgggcgccgg gtcgcttccg gcgccgagca
     3121 cgggcgcggt gccccatggg ccgcgcccgt gctgtttttc gcccggcttg cccctcgggg
     3181 tatcatgcga aacggtcatg aagaggcccg cgtcctgctg accgtcggcc agcttaccga
     3241 gccgttgacc ggtcggcact gggatgcgcc ggccgtgacg cgcgaggtct cggcacgggc
     3301 cgcccgctat cggcgccgcg ggctccgacc cggcgaccgg gtgctgctgc acttcggcaa
     3361 ccggctcgag ttcttcgccg agctcctggc cgtctggcgg tcgggcgccg tcgcggtgcc
     3421 ggtggacggc accctgacgc gcgtcgagct cgagcgcctc gtggagaccg tcgcgccccg
     3481 cttctcggtg gtggacgaca ccaccggcgg ggcgatcgcc cgcgcgctct cgcccggggc
     3541 gacggtggtg gacgcgaccg agccgctccc gcccgccgat ctgcccgagg acgaggcgcg
     3601 tcctgcgcgc gacgcgctcg tgctcttcac ctccggatcg agcggcgtgc ccaagggcgt
     3661 ggtgcacacc cacgcctcgc tggccgcccg gtggagcgcg ctgcggcaga agctcgggct
     3721 cgtggcctac gcgcgcacgc tctgcctgct gccgacccac ttcggtcacg gcctcatctg
     3781 caacagcctc tttccgtggc tcgccggtca ggatctcttc atcacgccgc ccttccggcc
     3841 cgatctcttg ctccgcctgg gcgggatcat cgacgagcac cggatcagct tcctctcctc
     3901 ggtgcccgcg atgtggcgcc tcgcgctccg ggtggcccgc ccaccgcgcg gcggcacgct
     3961 gcatcgcatc cactgcggct cggcaccgct gcccgccacc ctgtgggaga gcgttcgcgg
     4021 gtgggcgggg acaccggagg tcctcaatac ctacgggctc accgagaccg ggagctgggt
     4081 ggccggcacc tcggacggcc cattcacgcc ggcagacggg ctcatcggcc cgccgtgggg
     4141 cgcgatcctg cacgtgcagc gcgccgacgc catgccgtgc gcgatgggcg aggtcggcga
     4201 ggtgtggatc gagagccccg cgctcatgcg cggctacctc gggcaggacg agctgacacg
     4261 acgggcgatc caacacggcc gcttcgcgac cggggacctc ggcctgctcg acgagcgcgg
     4321 ctggctccac ctgcgcgggc gcgagcgtga cgagatcaac aagggcggcc tcaagatctc
     4381 cccggccgag gtggacgcgg ccgccgaggg ctgccgtcgc gtcgccgacg tctgcgcctt
     4441 cgcggtggac gatcccctct acggccagaa tgtgggcctc gcggtggcgc tcaccgagcc
     4501 cgacgacgcg gcggtccgtg aagtgtacgg ctggatccgc gagcgactcg ccgagggcaa
     4561 gctgcccgcg cactggtggc tggtggacac gatcccgcgc tccgagcgcg gcaaggtgag
     4621 ccgcgacgcg gtgcgggagg cctgcgcggg ccgaccgccg ctcgacctta cgcggatcct
     4681 gcaggagcgc tgaatgcgcc accgccaccg cgagcgggtt ctcgacttcc tgcgcaccgt
     4741 ccagaagccc gaccggcccc tcgacacggt ggcggagacc gacagcctcg tggagagcgg
     4801 gctgatcgat tccctcgccc tgctcgagat cgtggccttc ctggaggagc agtacgggat
     4861 cgacttcgcg gccgtcgggc tcgagccgga gcagctcacc tcggtgagcg gtatcctcga
     4921 cctgatcgag cgggaggccc ggtgacggcg gcgaccatgg gagccgccgc ccggcggctc
     4981 cgcagctttc acgaggaccc ctccgtcatc gcccgctggc aggcgtggac ggggccgctc
     5041 ctgacccggc tgaccccgcc gcggcgccgc gcgctgctgg ccctggccgc gctctgggtg
     5101 gtgatcaagc gacccctcaa ggaactagcg cccgcaatcg agctgcggag cccgaaccgc
     5161 ccgacggccg ccacggcggt ggtcctgctc tgcctggcca tcgtcgccgt catgtacctc
     5221 gtggcccggc gcttcccggc gctgcccgcg gtggtacgcg cccgcccgca gatctggctg
     5281 cacggtctct tctgggttct ggtgctcgtc tgctggctcg tcccgaaggg gcgcaccgtg
     5341 accacgctcg cgctcgccgg gctcgtgctc gcgctccctt tcctgctgtg gcgcatcggc
     5401 tacatgctca tgtccggcca gcgcggccgt atcgccggca cgcgcttctc cgaccacctg
     5461 ctctatctct atccggcgta cggcggcacc aacacgccct acggcaaggg tatcgaccac
     5521 ctggcgcgcc acgaggccca gacggacgag gcgctcgcgc gctcgcagct cgccggcctc
     5581 aagctcctgg ccctggcgtg gatctatgac cgggggctcg atctgctggc cgggctgatc
     5641 cacggccagc cgagcgggtt gctcggcgca tacggcctcg gcctgccccg gctcgagacg
     5701 ctcatgccca tgggcgcgcg ggtgccctgg ctcaccgcgt gggccgcggt ctacgccgag
     5761 ctggtctcgg acgtgctcaa gctcgcggtg cggggccacg agatcgtggg ggtcctgcgg
     5821 atcttcggct tcaacgtctt ccgcaacacc tacaagccgc tcctggctcc gtcgctggtc
     5881 gagttctgga accgttacca ctactatttc aaggagctgc tcgtcgagtt ctttttcttc
     5941 ccgacctacg tccggcgcgt ccggaagtcg ccccgcctgc gcatgctcgc ggccaccctc
     6001 gcggcggcgg gcttcggcaa cttctactac cacctcctgc agcaggacgc gttcatgctg
     6061 accggaagct gggccgccct gcacccgtgg atcgagacgc gcgcgctcta ctgcctgatc
     6121 ctggccctcg gcatctacgt gtccatgcgg cgcgagcagc agcgccgggg cacgccggcc
     6181 gccgcgcacg cgggctcggc ggtgcggctg cggcggatcg ccggcgtctg gacgttcttc
     6241 gccctgatcg gcatctgggg cgaggcgggc accgcgtcgt tcgcccagcg cgccgacttc
     6301 gtcctcgcgc tcttcgggct ccgatgagcc ggacgatccc gggttccgtg cggctcgccg
     6361 ccgccgccct cggcctggtg gcgctcaacc tcgtcctcct ggccgtctac cgcctcctct
     6421 tcgtcgcgtg gttcgcccca cgcgccgcgt ggggtgaggt acccgccgtg ctcctccacg
     6481 ggctccgcct ggacgtggcg cttctgggcc tcgagctgct cgtcatcggc ggcctcaccc
     6541 tgctcacgcg tcacgcgcgc ggccacgtcc tagtggcgag cctctggctg ctcaccacgc
     6601 tcaacgtgac ggtggcggcc gtcaatctcc tcttcgttcg cgagcgcaac cagcatctct
     6661 gggagatgct cttcgcgtat ctggccgagc cccgtgacat ctgggtggcg ctgcggccct
     6721 tcctgtgggg gcatccgctc ctggtcgcgc tggtgatcct cgccctggcg ggcctcgtca
     6781 tgctcgccac ccgccacgtc cggcggctcg gcccccaccg ctacgatctg tggcgcccct
     6841 ggtacgttcc ctccctcgcg ctcggcgcgc tcgcgctgtt cgcgcttccg atggcgcagc
     6901 ccatcacggt caagcaggtg ggcggccggg ccaagaccga ggtcgcgtgg atcgcgagcc
     6961 ggcaccagat ggcgctggac gactacgtgc tcaatcaggc cgtggtcaac ccgctctggg
     7021 atctggtccg cgagtacctc ccggtggcca tcacgggcgg gcgtcccccg taccggctcg
     7081 aaaccgcgga agcgctcccg ctcgcccagc ggctgctcgg catccccgag ggcgatcggc
     7141 cgtatccgct cctgcgcacc cttcgcggga ccggcggcct cgggatccgg aacgtggtgc
     7201 tgatccaggt cgaggggctc ggcgccaccc tgctcgagcg cgacgagccg cagggtccgg
     7261 tcctgccgtt cctccgctcg ctcgcggccg agggcctcta cttcccccgg gtgtaccaga
     7321 gcttcccgtc cacggacggc gcggtcttcg cgacgctcac cagtcttcac tggacccacg
     7381 ccctgggcgg cagcggcgtg cgcctctccc agtcggtcat gggcacctac ttcgcctcgc
     7441 tcccgcgcct gctgaccgcg ccgggagccc agcactacgc cttctccggc tttcgccacc
     7501 gcaccgccga gctcacgagc ttccagcgca acctgggctt ccgcgccgcc ggcttcgacg
     7561 cgcttggcac gcgcctcggg ccgga
//
LOCUS       DASUFA010000106        12209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1157996, whole genome shotgun sequence.
ACCESSION   DASUFA010000106 DASUFA010000000
VERSION     DASUFA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157996"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="VFX87_03965"
     CDS             <1..1850
                     /locus_tag="VFX87_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4994096.1"
                     /translation="AAFKAAPVVVKKRIVNQRLVANAMEPRACVARYDDSTGDWTLWV
                     TSQNPHVHRLLMTAFVLGIPEHKVRVIAPDVGGGFGSKIFLYNEETVCTWATKQIKRP
                     IRWTSSRREAFQTDAHGRDHVTDAEIALAKDGKFLGLRVKTTANLGAYLSTFAPAVPT
                     YLYATLLNGVYEWPAIHAEVTGVFTNTTPVDAYRGAGRPEACYVLERMVDAAAAALKM
                     DPADIRKKNFIPKFADGYQTKVALSYDSGNYLGAFDKLLGMLDYKKFRADQAAARAQG
                     RLMGIGFSTYIEACSIAPSKVVGSLGAQAGLWESGKVQVHPTGKVSVFTGSHSHGQGH
                     ETTMAQIVADSLGIPMDDVEIVHGDTGRVPFGMGTYGSRSASVGGTAIVMSLDKIKEK
                     GKKIAAHLLEANPKDMEYVGGQFVVKGSPQKAIPFGQVALTAYVPHNYPEGVEPGLEE
                     TSFYDPSNFCFPFGAHACVVEVDPDTGHVKILRYLAVDDVGNVINPMIVDGMVHGGIA
                     QGVAQALWEEAVYDNASGQLVSGSMMDYTLPKADMLPFYETGRTETPTLVNPLGVKGA
                     GETGTIASTPAVVNAVVDALSGLGVDHIDAMPLTPERVWKTAQAAKSRK"
     gene            1865..2728
                     /locus_tag="VFX87_03970"
     CDS             1865..2728
                     /locus_tag="VFX87_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX4994097.1"
                     /translation="MYPAQFEYHTPGTVREALDLLGRHKDDAKLLAGGHSLLPAMKLR
                     LAQPKHLVDLRKVSGLSGIKEEGGALVIGAMTTHYQVESSAVVKSKCPMLAAVAAQIG
                     DPMVRNMGTIGGSLAHADPAADYPAAIIALGAEMVAEGPKGKRTIKADDFFKGLLTTA
                     LAENEILTEVRVPVMAGKSGAAYMKFPHPASRFAVVGVAAVLSVDGGKVGKASVAVTG
                     AGTKAVRAKGVEAAITGKSPDAASIQAAAEKAAEGVDVQADLQGTVEYKSHLVRVFAK
                     RAMEAAAAAVK"
     gene            2803..3405
                     /locus_tag="VFX87_03975"
     CDS             2803..3405
                     /locus_tag="VFX87_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4994098.1"
                     /translation="MIAAVVLAAGLSRRMGQPKLLMKVGGRALVRYVVESVLSGGVDS
                     VWVVTGPDVEPVEAALAGIEVQIAVNPAPEEGQAGSVRTGIAALPASVDAVLIALGDQ
                     PLLAPSIIPALLAARRTTPKLIVAPRYRDGQGNPVVFKREIFPELLRLTGDQGARPII
                     QKEPTRVEWVELDLPMPPDVDTPDDYEKIRANLRAGYHAV"
     gene            3418..4308
                     /locus_tag="VFX87_03980"
     CDS             3418..4308
                     /locus_tag="VFX87_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4994099.1"
                     /translation="MRAEIEKIQQMMEKADYVTDPAIATSVHLAMTLGKPLLIEGHAG
                     VGKTEIAKVMARMLDTNLIRLQCYEGLDAAQALYEWNYPKQLLHIKLEEATTHSLREK
                     EATIFSEPFLIRRPLLQAISQAGKPPVLLIDEVDRSDEEFEAFLLEVLAEFQVTIPEI
                     GTIKTTHPPHVILTSNRVRELSDALRRRCLYLWVDFPGFDKEVRIVTRKVPGVNEALA
                     RQIARFMESVRSIRLSKVPGVAESLDWAQALASLHANHLDEDLVLETLGCVLKDADDI
                     KRFKRELTASGLKRFVAAEG"
     gene            4310..5464
                     /locus_tag="VFX87_03985"
     CDS             4310..5464
                     /locus_tag="VFX87_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4994100.1"
                     /translation="MAGPRDLTTAVLRFGQMLRAAGLPLTIGELMDGVRALAVVDLLD
                     RQDVYLALRTTLVARHEEFPAFDRCFDVFWRFQAEEGQGLDGLTSPTDAAIPEEHAGN
                     TGAEAAQKKVSVALEGWEEQGDDDGEPLEVPGVSDREILMDQDFSAFPVEDLDEVARL
                     TILIAKRLARRISRRKRPTRRGGAIDMRRSMRANLMKGEIIELRRKERRRRKVRLVLL
                     CDVSGSMDLYSRFLLQFLYALQNVFGRVETFTFSTRLTRVTDHLKGASYRQALAKLTE
                     VKDFSGGTRIGESLQEFNVRWGHLVDRHTIVLVLSDGWDTGEPEILANEMLTIKRKAG
                     RLIWLNPLLGNPSYEPLTRGMAAALPLVDHFAAAHNLASLRDLAGHLNLR"
     gene            5470..6558
                     /locus_tag="VFX87_03990"
     CDS             5470..6558
                     /locus_tag="VFX87_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX4994101.1"
                     /translation="MTELSEQLNQLKRNESKVTVATLVNTRGTTPRKEGAKMLVGEGG
                     RVLGSVTIGGCVDAQVIEESADVLGKNAPRLLELNLGDEEAWEIGLTCGGTIEVFLEP
                     VTLDDKDDTTLVYYERARVHAEQGGRAVIVTRLDAPHNGAKLLLFDHGGQEGTLGDAF
                     LDQRFTGEAREAMTGGKSRTLFLENVRAFAEVFTPPSTLVVVGASHVAMPLVTLAKTL
                     GFRTIVVDGRPRFATKDRFPDVDDLRIGVPSELVQQVPLIATTALVLVAHDYKYDLPV
                     LRHALSTPVGYIGLLGSSRRGAAILNLLREDGVAAEALLRVRVPIGLDLGAQSAPEIA
                     LSILAEALATQRNATGMPISEKVRRGLK"
     gene            6563..7606
                     /locus_tag="VFX87_03995"
     CDS             6563..7606
                     /locus_tag="VFX87_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994102.1"
                     /translation="MKAIAVPHGTATADALEGRIACHDVRDQAGKVAIAKGQTLDAAA
                     AARLLTLPWQEVHLLELEPGDVHEEPAGSRLAAAACGPNVEVKGYTGGQWTLAAQRRG
                     LVGVNAPAVAAVNGLEGMSIFTLYDLQPVESGEPVARAKITPLAIPERLVKDAEARAA
                     SAGGLVSVKPFRPHVLAALSRESLAPPQRQRFESSLTEKVDWFGSRLLPVRYAAGDAA
                     VVAAELEGLLAAGADILIAAGAGSLDPLDPVFAGLERLGGRMIRHGAPAHPGSLLWIA
                     EVRGHPVLGMPACGMFSQATTFDLVLPRILAGLPTGAAEIAALGHGGLLSRDSAYRFP
                     PYRKTGVRGELAE"
     gene            7608..7982
                     /locus_tag="VFX87_04000"
     CDS             7608..7982
                     /locus_tag="VFX87_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX4994103.1"
                     /translation="MALYSEVIRERFRRPLFRGAIERPDVAHEDVNPLCGDRVRIQGR
                     LADGRLAEARWTGDCCAICAASADLLLEAALGQPVGFARTQTAASLLERLDSDIRPSR
                     MKCVTLPLSVLQEAVSGQEVTR"
     gene            7979..9196
                     /locus_tag="VFX87_04005"
     CDS             7979..9196
                     /locus_tag="VFX87_04005"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX4994104.1"
                     /translation="MNLGEKTRRDFPILSRVVNGHPLVYLDSAASSQKPLAVIEAISR
                     YYETSHANVHRSIHTLGEEATERYEAARDAVRAFVGARSREEIIFTRGTTEGINLVAQ
                     AVGRTLEAGDEIIVSELEHHSNLIPWQMACRDRGAVLRAIPVVGEGYLDLEAFDRLLS
                     PRTRVVAVAHVSNVLGTINPVARIVERAHRVGALVLLDGAQAVPHLPLDLHAIGADFY
                     AFSGHKRLGPTGIGVLHGRREVLDKLEPAWGGGEMIKEVWIDHAQWNDLPWRFEPGTP
                     PIAEAVGLHAACEYLGKLGMSEVLAHEQALVAQCLDALGRIEGVTLYGPRNPEMKGAV
                     VAFNVEGLHPHDGAALLDQQGIAVRAGFHCAQPLMRCLGIAGTMRASFSVHSTAGEIE
                     RLAAAVATLRSTL"
     gene            9219..10169
                     /gene="speB"
                     /locus_tag="VFX87_04010"
     CDS             9219..10169
                     /gene="speB"
                     /locus_tag="VFX87_04010"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX4994105.1"
                     /translation="MDPSREHPKQRDGFASPRFAQPATFMRLPHVESPAGLDVALCGI
                     PFDGGCSYRSGARMGPRHVREQSSLIRPWNSALRVHPFERLRVADCGDVDAVPVSIEA
                     TFAAIERTLAPVVEARAVPLCVGGDHTVTLAVLRALAKRHGRLGLVHFDAHPDTWDRY
                     FGLPYYHGSTFRRAVEEGLIDGPRSIQVGIRGPLYGPEDFDFHREHAFEVIRIEDVKE
                     RGVASVVERLRRLVGGPVYVSFDVDACDPAYAPGTGTPEAGGLTSYEALSLLRGLRGI
                     ELAGGDIVEVSPPYDGPGAITGVLAANLLFELLSVVALAR"
     gene            10199..11518
                     /locus_tag="VFX87_04015"
     CDS             10199..11518
                     /locus_tag="VFX87_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX4994106.1"
                     /translation="MRSALHRPLLVTVAILLAFAALLSIAGPASAAQSVSLIELDSAI
                     TPVTVRLLANAIDRAQAAGAQALIVQLNTPGGLERSMRSMVQSILGSPIPVIVYVAPA
                     GARAASAGVFITMAAHVAAMAPATNIGAAHPVSVGGGGDKEMIKKVENDAAAFARTIA
                     TERGRNAEWAEKAVRSSVSATEREAVKLKIVDLVAESVPDLLARINGRTVKTARGQVT
                     LATKDAPVQVIEIRFRDRFLALISDPNIAYLLMMAGMLGIFFELSSPGAILPGVMGGI
                     CLILAFYAFQSLPVNWAGLLLLLFGVVLLVAEVKVVSHGVLAIGGVVAMLLGSLMLYD
                     SPETTGIRLSWYVVIPTVGTTAALVFFAVSMGIRALYRPSVTGESAMVGRLGVARSAL
                     APEGQVMIDGELWRAVSPDGTVSAGESVRVTAIDGLTLKVTRSAQRP"
     gene            11538..>12209
                     /locus_tag="VFX87_04020"
     CDS             11538..>12209
                     /locus_tag="VFX87_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX4994107.1"
                     /translation="MEGVQLLFGIIPVAIILLYIFFSSFRILREYERGVIFRFGRRTN
                     AIFNPGGDGTGPGLVLLIPFIDKMVKVSLRTITMDVPPQDVITRDNVSVKVNAVIFFR
                     VLDAAKAVISVEDYLYATSQMAQTTLRSVLGQQEMDDLLASREKINEELTRIIDQHTS
                     PWGVKVAAVEVKNVDLPQDMQRAMSKQAEAERERRAKVINAEGEFQAAAKLGEAAEVL
                     MRFPIA"
BASE COUNT         1850 a         4276 c         4332 g         1751 t
ORIGIN      
        1 aggcggcctt caaggcggcg ccggtcgtgg tgaagaagcg gatcgtgaac cagcgcctgg
       61 tcgccaacgc gatggagccg cgggcctgcg tggcgcgcta cgacgactcc accggcgact
      121 ggacgctctg ggtcacgtcc cagaaccccc acgtgcaccg gctgctcatg accgcgttcg
      181 tgctgggcat ccccgagcac aaggtgcggg tcatcgcgcc cgacgtgggc ggcggcttcg
      241 gctccaagat cttcctctac aacgaggaga cggtctgcac ctgggccacc aagcagatca
      301 agcggccgat ccgctggacc tcctcgcgcc gcgaggcctt ccagaccgac gcccacgggc
      361 gtgatcacgt gaccgacgcc gagatcgccc tggcgaagga cggcaagttc ctgggcctgc
      421 gggtgaagac caccgcgaac ctgggcgcgt atctgtccac gttcgcgccg gcggtgccga
      481 cctatctgta cgcgacgctg ctcaacggcg tctacgagtg gcccgccatc cacgcggagg
      541 tcaccggcgt cttcaccaac accacgccgg tggacgccta ccggggggcc gggcggcccg
      601 aggcctgcta cgtgctcgag cgcatggtgg acgccgccgc cgccgcgctc aagatggatc
      661 cagcggacat ccgcaagaag aacttcatcc cgaagttcgc cgacggctac cagaccaagg
      721 tggcgctgtc ctacgacagc ggcaactacc tgggcgcctt cgacaagctg ctgggcatgc
      781 tcgactacaa gaagttccgg gccgatcagg cggcggcgcg tgcgcagggc cggctgatgg
      841 gcatcggctt ctcgacctac atcgaggcct gcagcatcgc gccctccaag gtcgtcggct
      901 ccctgggggc ccaggccggc ctctgggagt ccggcaaggt tcaggtgcat cccaccggca
      961 aggtcagcgt gttcaccggc tcccactcgc acgggcaggg gcacgagacc accatggccc
     1021 agatcgtcgc cgattcgctc gggatcccga tggacgacgt ggagatcgtc cacggcgaca
     1081 ccggccgtgt gccgttcggc atgggcacct acggcagccg ctcggcctcg gtgggcggca
     1141 ccgccatcgt catgtcgctg gacaagatca aggagaaggg caagaagatc gccgcccacc
     1201 tgctcgaggc gaacccgaag gacatggagt acgtgggcgg ccagttcgtg gtcaagggct
     1261 cgccccagaa ggcgatcccg ttcggccagg tcgcgctcac cgcatacgtg ccgcacaact
     1321 atccggaagg cgtggagccg ggcctcgagg agacgagctt ctacgacccg tccaacttct
     1381 gcttcccgtt cggcgcccac gcctgcgtgg tcgaggtgga tccggatacc ggccacgtga
     1441 agatcctgcg atacctcgcg gtggacgacg tcggcaacgt gatcaacccc atgatcgtgg
     1501 acgggatggt ccacgggggc atcgcccagg gcgtggcgca ggcgctctgg gaggaagcgg
     1561 tgtacgacaa cgcctccggg cagctcgtca gcgggagcat gatggactac acgctcccca
     1621 aggcggacat gctgccgttc tacgagaccg gccgcaccga gacgcccacg ctcgtgaacc
     1681 cgctcggcgt caagggagct ggcgagacgg gcacgatcgc ctcgacgcct gcggtggtca
     1741 acgcggtggt ggatgccctg tcggggctcg gcgtggacca catcgacgcg atgccgctga
     1801 cgccggagcg ggtctggaaa accgcgcagg ccgccaaatc caggaagtaa ggggagacac
     1861 cgacatgtac ccagcccagt tcgagtacca cacgcccggc accgtgagag aggcgctgga
     1921 cctgctcggg cgccacaagg acgacgccaa gctgctggcg ggcggtcaca gcctgctgcc
     1981 ggccatgaag ctgcgtctgg cccagccgaa gcacctggta gacctgcgga aggtctccgg
     2041 cctctccggc atcaaggaag agggcggcgc gctcgtgatc ggggcgatga ccacccacta
     2101 ccaggtcgag tcctccgcgg tcgtgaagtc gaagtgcccg atgctcgccg cggtggccgc
     2161 tcagatcggg gacccgatgg tgcgcaacat gggcacgatc ggcggcagcc tggcccacgc
     2221 ggaccccgcc gccgactacc cggccgcgat catcgcgctc ggcgcggaga tggtggccga
     2281 gggccccaag ggcaagcgca cgatcaaggc cgatgacttc ttcaagggcc tgctcaccac
     2341 cgcgctcgcg gagaacgaga tcctgaccga ggtgcgcgtg ccggtcatgg cgggcaagtc
     2401 gggcgccgcc tacatgaagt tcccgcaccc ggcctcccgg tttgcggtgg tcggcgtcgc
     2461 ggcggtgctg agcgtggacg gcggcaaggt cggcaaggcc agcgtcgcgg tcaccggcgc
     2521 ggggaccaag gcggtgcgcg ccaagggggt ggaggccgcg atcaccggca agtcgcccga
     2581 cgcggcgtcc atccaggcgg cggccgagaa ggcggccgag ggcgtggacg tccaggccga
     2641 tctgcagggc acggtggagt acaagagcca cctggtccgg gtcttcgcca agcgggcgat
     2701 ggaggcggcc gctgcggccg tgaagtaagc cgctcgggtg agacgtccag tcaacgagcc
     2761 ccgcgggatg gcctccgcgg ggcgtcgttt ttggcgcgaa ggatgatcgc ggcggtcgtc
     2821 ctcgccgccg gcctctcgcg ccggatgggc cagcccaagc tgctcatgaa ggtgggcggc
     2881 cgggccctcg tccggtacgt cgtcgaaagc gtcctctcgg gcggcgtgga ctcggtctgg
     2941 gtcgtgaccg ggcccgacgt cgagccggtc gaggccgccc tggccggcat cgaggtgcag
     3001 atcgcggtga acccggcccc cgaggagggc caggccggct cggtgcgcac gggcatcgcc
     3061 gcgctgcccg cgtcggtgga cgcggtgctg atcgcgctcg gcgaccagcc gctgctcgcg
     3121 ccctccatca tcccggcgct gctggcggcc cgccgcacga cccccaagct catcgtggcc
     3181 ccgcgctacc gggacgggca gggcaacccg gtggtgttca agcgcgagat ctttcccgag
     3241 ctgctgcgcc tgacgggcga ccagggcgcg cggcccatca tccagaagga gccgacccgc
     3301 gtggagtggg tcgagctcga cctgcccatg ccgccggacg tggacacgcc cgacgactat
     3361 gagaagatcc gggcgaacct ccgggcaggc tatcacgcgg tgtagactga gagggccatg
     3421 agagccgaaa tcgagaagat ccagcagatg atggagaagg ccgactacgt cacggatccg
     3481 gccatcgcga cgtccgtgca cctggccatg acgctcggga agccgctcct catcgaggga
     3541 cacgcgggcg tcggcaagac cgagatcgcc aaggtgatgg cgcggatgct cgacaccaac
     3601 ctcattcgcc tgcagtgcta cgaggggctc gacgcggccc aggcgctcta cgagtggaac
     3661 tacccgaagc agctcctgca catcaagctc gaggaggcga ccacccacag ccttcgcgag
     3721 aaggaagcga cgatcttctc cgagcccttc ctgatccgcc gcccgctgct ccaggcgatc
     3781 agccaggcgg gcaagccgcc ggtgctgctg atcgacgagg tcgaccgaag cgatgaagaa
     3841 ttcgaggcat tcctcctcga ggtgctcgcc gagttccagg tgacgatccc cgagatcggc
     3901 acgatcaaga ccacgcatcc gccacacgtc atcctcacct cgaaccgcgt gcgcgagctc
     3961 tccgatgcgc tccgccgccg ctgcctctac ctgtgggtgg atttccccgg attcgacaag
     4021 gaagtccgta tcgtgacgcg caaggtgccg ggcgtgaacg aggcgctggc gcgccagatc
     4081 gcgcgcttca tggaaagcgt ccggagcatc cgcctctcca aggtgccggg ggtggcggag
     4141 agcctcgact gggcacaggc cctggcctcg ctccacgcca atcacctcga cgaggacctg
     4201 gtgctggaga cgctcggctg cgtgctcaag gacgccgacg acatcaagcg cttcaagcgg
     4261 gagctcacgg caagcggcct caagcgcttc gtggccgccg agggatagca tggccggacc
     4321 ccgcgacctc accaccgccg tcctccgctt cgggcagatg ctgcgggcgg cggggctgcc
     4381 gctcaccatc ggcgagctga tggacggcgt gcgggcgctg gcggtcgtgg acctgctgga
     4441 ccgccaggac gtctacctgg cgctgcgcac gaccctggtc gcccggcacg aggagttccc
     4501 cgccttcgac cgctgcttcg acgtcttctg gcgcttccag gccgaggagg ggcaggggct
     4561 cgacggcctc acgagcccga ccgacgccgc catccccgag gagcacgccg gcaataccgg
     4621 cgcggaagcg gcccagaaga aggtctcggt ggctctggaa gggtgggagg agcagggcga
     4681 cgacgacggg gagccgctcg aggttccggg ggtcagcgac agagaaattc tcatggatca
     4741 ggacttttcg gcctttccgg ttgaggatct agacgaggtg gcccggctca cgatcctcat
     4801 cgccaagcgg ctggcccggc gcatcagccg gcgcaagcgg ccgacccgcc ggggcggggc
     4861 catcgacatg cggcgcagca tgcgggccaa cctcatgaag ggcgagatca tcgagctgcg
     4921 gagaaaagag cggcggcggc gcaaggtccg actggtgctg ctgtgcgacg tgtcgggctc
     4981 gatggacctc tacagccgct tcctgctcca gttcctctac gcgctccaga acgtcttcgg
     5041 ccgcgtggag accttcacct tctcgacgcg cctcacgcgg gtgaccgacc atctcaaagg
     5101 ggcatcgtat cgccaggctt tggccaagct gacggaggtg aaggacttct ccggcgggac
     5161 ccggatcggg gagtcgttgc aggagttcaa tgtgcggtgg gggcacctgg tggaccggca
     5221 caccatcgtg ctggtgctct cggacggctg ggacaccggc gagcccgaga tcctggccaa
     5281 cgagatgctg acgatcaagc ggaaggcggg ccgcctcatc tggctcaacc cgctgctggg
     5341 caacccgtcc tacgagccgc tgacgcgtgg catggcggcg gcgctgccgc tcgtggacca
     5401 cttcgcggcg gcgcacaacc tggcgagcct gcgcgacctg gccggccatc tcaacctgag
     5461 gtagcggaca tgaccgagct ctccgagcag ctgaaccagc tcaagcggaa cgagtccaag
     5521 gtgacggtgg ccaccctcgt caacacgcgc ggcaccacgc cccggaagga aggagcgaag
     5581 atgctggtgg gcgagggcgg gcgggtgctc ggctccgtga ccatcggcgg ctgcgtggac
     5641 gcgcaggtca tcgaggagtc ggccgacgtg ctgggcaaga acgccccacg gctgctggag
     5701 ctgaacctgg gcgacgagga ggcgtgggag atcgggctca cctgcggggg caccatcgag
     5761 gtcttcctgg agccggtgac gctcgacgac aaggacgaca ccacgctggt ctactacgag
     5821 cgcgcccgcg tccacgccga gcagggcggg cgggccgtga tcgtgacccg cctcgatgcg
     5881 ccgcacaacg gcgccaagct cctgctgttc gaccacggcg ggcaagaggg cacgctcggc
     5941 gacgccttcc tcgaccagcg cttcaccggg gaggcccgcg aggccatgac cgggggcaag
     6001 tcgcggacgc tcttcctcga gaacgtgcgc gccttcgcag aggtcttcac gccgccgtcc
     6061 acgctggtcg tggtgggggc cagccacgtc gcgatgccgc tcgtcacgct ggccaagacg
     6121 ctcggcttcc gcaccatcgt ggtggacggc cggccccgct tcgccaccaa ggatcgcttc
     6181 ccggacgtgg acgatctccg gatcggcgtg ccgtccgagc tggtgcagca ggtcccgctg
     6241 atcgcgacca ccgcgctggt gctggtggcc cacgactaca agtacgatct cccggtgctg
     6301 cgccacgcgc tgagcacccc ggtgggctac atcgggctcc tgggcagcag ccggcgcggg
     6361 gcggcgatcc tgaacctgct gcgcgaggac ggcgtggccg cggaggccct gctccgcgtg
     6421 cgggtgccga tcggcctcga cctgggggcc cagtcggcgc ccgagatcgc gctcagcatc
     6481 ctggccgagg ccctggcgac tcagcgcaac gccaccggca tgccgatctc cgagaaagtg
     6541 cggcgcggcc tcaagtaacg tcatgaaagc catcgcggtc ccgcacggca cggccacggc
     6601 cgacgcgctc gaggggcgca tcgcgtgcca tgacgtgcgc gaccaggccg ggaaggtggc
     6661 catcgccaag gggcagacgc tcgacgcggc cgccgccgcc cggctgctca cgctgccgtg
     6721 gcaggaggtg cacctgctcg agctggagcc gggcgacgtg cacgaggagc cggccggctc
     6781 gcgcctggcc gccgcggcct gcggccccaa cgtggaggtc aagggctata ccggcgggca
     6841 gtggacgctc gcggcacagc ggcgcggcct cgtcggcgtg aacgcgcccg cggtagccgc
     6901 ggtcaacggc ctcgagggca tgtcgatctt cacgctctac gatctgcagc cggtggagtc
     6961 gggcgagccg gtggcccgcg ccaagatcac cccgctggcc atcccggagc ggctcgtgaa
     7021 ggacgcggag gcccgcgcgg cgagcgcggg cgggctcgtc tcggtgaagc cctttcggcc
     7081 ccacgtgctg gccgcgctga gccgggagag cctcgccccg ccgcagcgcc agcgcttcga
     7141 gtcgtccctg accgagaagg tggactggtt cggctcgcgg ctcttgccgg tgcggtacgc
     7201 ggcgggggac gcggccgtcg tcgccgccga gctggagggc ctgctggccg ccggcgccga
     7261 tatcctgatc gcggcgggcg ccggctcgct cgacccgctc gatccggtgt tcgcggggct
     7321 cgagcgcctg ggcggccgca tgatccggca cggcgcgccg gcccatccgg ggagcctgct
     7381 gtggatcgcc gaagtccgcg ggcacccggt gctcggcatg cccgcctgcg ggatgttctc
     7441 gcaggccacc acgttcgatc tggtgctgcc gcgcatcctg gccgggctcc cgaccggggc
     7501 ggccgaaatc gccgccctcg gccacggcgg cctgctctcg cgcgacagcg cctaccgctt
     7561 cccgccctac cgcaagaccg gggtgcgcgg ggagctggcc gagtgacatg gcgctctaca
     7621 gcgaggtgat ccgggagcgc tttcgccggc cgctcttccg gggtgcgatc gagcgcccgg
     7681 acgtcgccca cgaggacgtg aacccgctct gcggcgaccg cgtgcgcatc cagggcaggc
     7741 tcgccgacgg gcggctggcg gaggcccgct ggaccggcga ctgctgcgcg atctgcgccg
     7801 cctccgccga tctgctgctc gaggcggcgc tcggccagcc ggtgggcttc gcgcggaccc
     7861 agacggccgc gagcctgctg gagcggctcg attccgatat ccggccgtca cggatgaagt
     7921 gcgtgacgct cccgctgagc gtcctgcagg aggccgtcag cggtcaggag gtgacgcgat
     7981 gaacctgggc gagaagaccc gccgcgactt tcccatcctc tcccgggtcg tgaacgggca
     8041 tcccctcgtg tatctcgatt ccgccgcctc cagccagaag ccgctcgcgg tgatcgaggc
     8101 catctcgcgc tactacgaga cgagccacgc caacgtccac cgctccatcc acaccctggg
     8161 cgaggaggcc accgagcggt acgaggcggc ccgggacgcg gtgcgcgcct tcgtgggcgc
     8221 ccggagccgc gaggagatca tcttcacgcg cggcaccacc gagggcatca acctcgtggc
     8281 gcaggcggtg gggcggacgc tcgaagccgg cgacgagatc atcgtcagcg agctcgagca
     8341 ccactcgaac ctgatcccgt ggcagatggc ctgccgcgac cggggcgcgg tgctgcgggc
     8401 gatcccggtg gtcggggaag gctatctcga cctcgaggcc ttcgaccgcc tgctctcgcc
     8461 gcgcacgcgc gtggtggcgg tcgcccacgt ctcgaacgtg ctcggcacga tcaacccggt
     8521 ggcccggatc gtggagcgcg cgcatcgggt cggcgccctg gtgctgctcg acggcgccca
     8581 ggcggtgccg catctcccgc tcgacctgca cgcgatcggc gccgatttct acgccttctc
     8641 cggccacaag aggctcgggc caaccggcat cggcgtgctc cacgggcgcc gcgaggtgct
     8701 ggacaagctc gagcccgcct ggggcggcgg cgagatgatc aaggaggtct ggatcgacca
     8761 cgcgcagtgg aacgacctgc cgtggcgctt cgagccgggc acgcccccga tcgcggaggc
     8821 ggtgggactg cacgcggcct gcgagtacct cggcaagctc ggcatgagcg aggtgctggc
     8881 ccacgagcag gcgctcgtcg cccagtgcct ggacgcgctc gggcgcatcg agggcgtcac
     8941 gctctacggc ccgcgcaatc ccgagatgaa gggcgcggtg gtggccttca acgtcgaggg
     9001 gctacacccc cacgacggcg ccgcgctgct cgaccagcag ggaatcgcgg tgcgcgcggg
     9061 ctttcactgt gcccagccgc tgatgcggtg tctcggcatt gcgggcacga tgcgcgcgag
     9121 cttctcggtc cactccacgg ccggcgagat cgagcgcctg gccgcggcgg tggcgaccct
     9181 gcgcagcacg ctctagccgc cccggcgccg cggctcccat ggatccctcc cgcgagcatc
     9241 cgaagcagcg cgacggcttc gcgtctcctc gcttcgcgca gccggcgacc ttcatgcggc
     9301 tgccgcacgt ggagtcgccc gcggggctcg acgtggccct ctgcggcatt cccttcgacg
     9361 gcggctgctc ctaccgctcg ggcgcgcgca tgggcccgcg ccacgtgcgc gagcagtcct
     9421 cgctgatccg gccgtggaac tcggccctgc gcgtgcaccc cttcgagcgt ctccgggtcg
     9481 ccgactgcgg cgacgtggac gcggtgccgg tctcgatcga ggcgaccttc gcggccatcg
     9541 agcggacgct ggcgccggtg gtggaggcac gcgcggtccc gctctgcgtg gggggcgatc
     9601 acaccgtgac cctggcggtg ctgcgggcgc tggcgaagcg ccacgggcgc ctcggcctcg
     9661 tgcacttcga cgcccacccg gacacctggg accgctactt cggcctgccc tactaccacg
     9721 gcagcacgtt ccgccgggcg gtcgaggagg ggctgatcga cggcccgcgc tccatccagg
     9781 tgggcatccg cgggccgctc tacggccccg aggacttcga cttccaccgc gagcacgcct
     9841 tcgaggtgat ccgtatcgag gacgtcaagg agcgcggggt cgcctccgtg gtcgagcggc
     9901 tgcggcgcct ggtcggcggg cccgtctacg tctccttcga cgtggacgcg tgcgatcccg
     9961 cctacgcgcc ggggacgggg acgcccgagg cgggcggcct cacgtcgtac gaggccctga
    10021 gcctcctgcg cgggctgcgc gggatcgagc tggccggggg cgacatcgtc gaggtctcgc
    10081 cgccctacga cgggcccggc gccatcaccg gcgtgctggc cgccaacctg ctcttcgagc
    10141 tgctctcggt ggtcgccctc gcccgctagg gcgcgtcccc gcgtgctaag atgacgccgt
    10201 gagatcggcc ttgcaccggc ctctcctcgt caccgtcgcc atcctgctgg ccttcgccgc
    10261 gctgctctcc atcgccggcc cggcctccgc ggctcaatcc gtgtcgctga tcgagctgga
    10321 ctccgccatc accccggtca cggtccgcct gctggcgaac gcgatcgacc gggcgcaggc
    10381 ggccggcgcc caggccctca tcgtgcagct caacacgccg ggcgggctcg agcggtccat
    10441 gcggtcgatg gtgcagagca tcctgggctc gcccatcccg gtgatcgtct acgtcgcgcc
    10501 ggccggggcg cgggccgcct ccgcgggggt cttcatcacg atggccgcgc acgtggccgc
    10561 gatggccccc gccaccaaca tcggggccgc gcacccggtg tcggtcggcg gcggcgggga
    10621 caaggagatg atcaagaagg tcgagaacga cgccgcggcc ttcgcgcgca ccatcgccac
    10681 cgagcggggg cgcaacgccg agtgggccga gaaggcggta cgctcgtcgg tctcggccac
    10741 cgagcgcgag gcggtcaagc tcaagatcgt ggacctggtg gccgagagcg tgccggatct
    10801 cctcgcccgg atcaacggcc gcaccgtcaa gacggcgcgc ggccaggtca cgctcgccac
    10861 caaggacgcg ccggtccagg tcatcgagat acgcttccgc gaccgcttcc tcgccctgat
    10921 ctcggacccc aacatcgcct acctcctgat gatggcgggc atgctcggca tcttcttcga
    10981 gctgtccagc ccgggcgcga tcctgcccgg cgtcatgggc ggcatctgcc tgatcctggc
    11041 cttctacgct ttccagagcc tgccggtgaa ctgggccggg ctcctgctgc tgctcttcgg
    11101 cgtcgtcctg ctcgtcgccg aggtcaaggt ggtgagccac ggcgtcctcg cgatcggcgg
    11161 cgtggtcgcc atgctgctcg ggtcgctcat gctctacgac tcgcccgaga cgaccgggat
    11221 ccggctgtcg tggtacgtgg tgatccccac ggtgggcacg accgcggcgc tggtcttctt
    11281 cgcggtgtcc atgggcatcc gcgcgctgta ccggccctcg gtgaccggcg aatccgccat
    11341 ggtggggcgg ctcggggtgg cgcggtcggc gctggcgccc gaggggcagg tgatgatcga
    11401 cggcgagctg tggcgggcgg tgagcccgga cgggaccgtg tcggcgggcg agtccgtccg
    11461 ggtcacggcc atcgatggcc tcacgctcaa ggtcaccagg tcggcgcagc gcccctagca
    11521 ctcccaggag gccgcacatg gaaggcgtcc agctcctctt cggcatcatc ccggtggcga
    11581 tcatcctgct ctacatcttc ttctcctcgt tccgcatcct gcgggagtac gagcggggtg
    11641 tgatcttccg gttcggccgg cggaccaacg ccatcttcaa tccgggcggc gacggcaccg
    11701 gcccgggcct cgtgctgctg atcccgttca tcgacaagat ggtgaaggtg agcctccgca
    11761 cgatcaccat ggacgtgccg ccccaggacg tgatcacgcg cgacaacgtc tcggtgaagg
    11821 tgaacgcggt catcttcttc cgcgtgctcg acgcggccaa ggcggtcatc agcgtggagg
    11881 actatctcta cgccacctcc cagatggcgc agaccacgct ccggagcgtc ctgggccagc
    11941 aggagatgga cgatctcctg gcctcgcgcg agaagatcaa cgaggagctg acccggatca
    12001 tcgaccagca cacgagcccg tggggcgtga aggtggcggc ggtggaggtg aagaatgtgg
    12061 acctgcccca ggacatgcag cgcgccatgt ccaagcaggc cgaggccgag cgcgagcggc
    12121 gcgccaaggt gatcaacgcg gagggcgagt tccaggccgc cgccaagctg ggggaggccg
    12181 ccgaggtgct catgcgcttc ccgatcgcg
//
LOCUS       DASUFA010000107         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_598308, whole genome shotgun sequence.
ACCESSION   DASUFA010000107 DASUFA010000000
VERSION     DASUFA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598308"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFX87_04025"
     CDS             complement(<1..784)
                     /locus_tag="VFX87_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994108.1"
                     /translation="MSAWPARVVLLGASGHIGRALTARLGREGIPVIPHTSKTLDLTR
                     FEALGALDGALGPDTALVFASALTPDRGQTPETFLANVAMAANLAKYLPGHAPGLCVY
                     VSSDAVYGFDVNPVTESTPVSPGGYYALGKYSGERVMEYAARAGGFPLLTLRVTGVYG
                     PGDPHGGYGPNAFARTLARDRGLRIFGQGEEERDHLYVDDVAGILAGLIRARATGLLN
                     VATGESRSFADVVKVIRRLAPYEVTVTSAPRGGAVTHRRFDTA"
     gene            complement(781..1380)
                     /locus_tag="VFX87_04030"
     CDS             complement(781..1380)
                     /locus_tag="VFX87_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX4994109.1"
                     /translation="MDFIARYLAEAGRVAAAIDQAAVARLVDLLVGVRDGGGRLFILG
                     VGGSAGNASHAVNDFRKIAGFEAYAPTDNVSELTARINDDGWDTSFANWLKGSRLSGR
                     DAVLVFSVGGGDAERGVSTNLVRALEHAKAVGARVAGIVGRDGGFTAKVADACVIVPT
                     VSPDLVTAHAEAFQAVVWHCLVSHPRLKASAMKWESLGR"
     gene            complement(1352..1912)
                     /locus_tag="VFX87_04035"
     CDS             complement(1352..1912)
                     /locus_tag="VFX87_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX4994110.1"
                     /translation="MTRRAVFLDRDGVLVETLMREGRAFAALTLDEFRLCSDTGPQVA
                     RLRAAGLLPIVFTNQPEMARGTLSATTLGRMHERLRAELDVADIFVCPHDDGADCACR
                     KPRPGMLHAAAAKWGINLLASFVVGDRWRDIDAGRAVGSYSVLLDRPYSDCAVADARV
                     SSLAEAVDAVLARAESSHGLHRPLSR"
     gene            complement(1917..>2617)
                     /locus_tag="VFX87_04040"
     CDS             complement(1917..>2617)
                     /locus_tag="VFX87_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX4994111.1"
                     /translation="LTKTAINLYLFGAVTYANTLADLCEEVGADWSEIAPALRLDRRI
                     GPAAYIRPSLGVAGGNLERDLVTLRGLCAAHGVDAAYIETLIQYNGRRYQWVHRQLER
                     RVLGDARPPVIAVWGLAYKKDTRSTKNSMALRVIGDLRGRAEIRAYDPLVGASEINGA
                     ATVLPDREATLAGADCLLILTDWDEFAATPRDAFKAMRRPLVIDCVGVVDPARTDLAG
                     VEFLTMGRPSGRAR"
BASE COUNT          383 a          946 c          948 g          340 t
ORIGIN      
        1 ccgcggtgtc gaagcggcga tgcgtgaccg cccccccgcg cggggcgcta gtcaccgtca
       61 cctcgtaggg cgccaggcgc cggatcacct tcaccacgtc ggcgaaggac cggctctcgc
      121 cggtggccac gttgagaagc ccggtggcgc gcgcccggat gaggccggcc aagattccgg
      181 ccacgtcgtc gacgtagagg tggtcgcgct cctcctcgcc ttgaccgaag atgcgcaggc
      241 cgcggtcgcg cgcgagggtg cgggcgaagg cattgggccc atagccgcca tgggggtcgc
      301 ccggaccgta gacaccggtc acccggagcg tgaggagggg aaagccgccg gcgcgggccg
      361 cgtactccat cacccgctcg cccgagtact tgcccagcgc gtagtagccg cccgggctca
      421 ccggcgtgga ctcggtgacg ggattgacgt cgaatccata gacggcgtcc gagctcacgt
      481 acacgcagag ccccggcgcg tgccccggga ggtacttcgc gagattggcg gccatggcca
      541 cattggccag gaaggtctcg ggcgtctgcc cgcgatccgg ggtcagcgcg gaggcgaaga
      601 cgagggcggt gtcggggccc agcgcgccgt ccagcgcgcc cagcgcctcg aagcgcgtga
      661 ggtcgagggt cttcgacgtg tgcgggatca cggggatccc ctcccggccg aggcgcgcgg
      721 tgagcgcgcg gccgatatgc ccgctggccc ccagcagcac cacccgcgcc ggccacgcac
      781 tcatcgcccg aggctctccc acttcatggc ggacgccttg agccgaggat gcgacacgag
      841 gcagtgccac accaccgcct ggaaggcctc cgcgtgggcg gtgaccagat ccgggctcac
      901 ggtgggaacg atgacgcagg catcggccac cttcgcggtg aacccaccgt cgcggcccac
      961 gatgcccgcc acccgcgcgc ccaccgcctt ggcgtgctcg agcgcccgca ccaggttggt
     1021 gctgacgccg cgctccgcgt cgccgccgcc caccgagaag acgagcaccg cgtcccggcc
     1081 ggagaggcgg ctgcccttga gccagttggc gaacgacgtg tcccagccgt cgtcgttgat
     1141 gcgggcggtg agctcggaca cgttgtcggt gggcgcgtac gcctcgaagc cggcgatctt
     1201 ccggaagtcg ttcaccgcat gggacgcatt tcccgcgctc ccgcccactc ccaggatgaa
     1261 gaggcggccg cctccgtcgc gcaccccgac gaggaggtcc acgaggcggg ccaccgcggc
     1321 ctggtcgatg gcggcggcga cgcgcccggc ttcagcgaga tagcgggcga tgaagtccat
     1381 gggagctctc ggcgcgcgcc agcacggcgt cgacggcctc ggccaggctc gacacgcggg
     1441 cgtcggcgac cgcgcagtca ctgtagggtc ggtccaggag gacggagtag gagcccaccg
     1501 cgcggccggc gtcgatgtcg cgccagcggt cgcccaccac gaaggacgcg aggagattga
     1561 tcccccactt ggccgccgcc gcgtggagca tgccgggccg gggcttccgg cacgcgcaat
     1621 ccgccccgtc gtcgtggggg cacacgaaga tgtcggccac gtcgagctcg gcccgcagcc
     1681 gctcgtgcat gcgccccagc gtggtcgcgc tcagcgtccc gcgcgccatt tccggctggt
     1741 tggtgaagac gatgggcagg agccccgccg cccggagccg cgccacctgg gggccggtgt
     1801 ccgagcagag gcggaactcg tcgagcgtga gcgcggcgaa ggcgcggccc tcgcgcatca
     1861 gggtctcgac cagcaccccg tcgcgatcca ggaacacggc ccgccgggtc acggtgtcac
     1921 cgcgcccttc ccgacggccg ccccatggtc aggaactcga cgccggcgag atcggtgcgg
     1981 gcggggtcca ccacgccgac acagtcgatc accagcggcc ggcgcatggc cttgaaggca
     2041 tcgcgcggcg tcgcggcgaa ctcgtcccag tcggtgagga tgaggaggca gtccgcgccg
     2101 gccagcgtcg cctcgcgatc cggcagcacc gtggccgccc cgttgatctc ggacgctccc
     2161 acgagaggat cgtaggctcg gatctcggcg cggccccgca ggtcgccgat cacccgcagc
     2221 gccatggagt tcttggtcga gcgcgtgtcc ttcttgtagg cgagccccca caccgcgatc
     2281 accggcggcc gggcgtcccc gagcacgcgc cgctcgagct gccggtgcac ccactgatag
     2341 cggcggccgt tgtactggat cagggtctcg atataggcgg cgtccactcc gtgggccgcg
     2401 cagagcccgc gcagcgtgac cagatcacgc tccagattgc cgccggcgac gccgaggctg
     2461 ggccggatat aggcggcggg cccgatgcgc cggtccaggc gcagggccgg cgcgatctca
     2521 gaccagtccg cgcccacctc ctcgcagagg tcggcgagcg tgttcgcgta ggtcaccgca
     2581 ccgaagaggt agaggttgat ggcggtcttg gtgagct
//
LOCUS       DASUFA010000108         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_356306, whole genome shotgun sequence.
ACCESSION   DASUFA010000108 DASUFA010000000
VERSION     DASUFA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356306"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFX87_04045"
     CDS             <1..718
                     /locus_tag="VFX87_04045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994112.1"
                     /translation="RDLDPIARAGPGDVRKSLLHLQCHFGMDTIRWAQQGAMVTGVDF
                     SEDAVIAARALAARMGVSATFVHCDLYELPAKLEGRFDVVFTSHGVLCWLPDLERWAQ
                     LIARFVAPGGLFYIVEAHPFLQIFNDQLAEPDLRLLYPYFHGPEPFRYEHPGCFSTPD
                     APITSVEHVWLHTLSDVIGALARAGLRITAFEEYPFLAWRFFPWMEQGPDGWWRLPED
                     PRLPLGRGSLPLMFSLKATP"
     gene            751..1611
                     /locus_tag="VFX87_04050"
     CDS             751..1611
                     /locus_tag="VFX87_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994113.1"
                     /translation="MDGVLAGAIYALIALGFVVVYKASGAINFALGEWALLGSRMTAT
                     GVHAVGLGLTGAIASASVGMVAVALAFNRLVLRPMVGRPLLSVIMVTLGSGVLIRGLT
                     PLAFGAMPAAIPFPAPPAPLEMWGVPVAAERLASAVIAVLCIALVGGLFRFTRTGLAL
                     RAIADDRQIAMAMGIDVHRHVSLAWAMLGAISVVTGALWTITSGGAFGVVIVGLKVFP
                     ILIIGGLDSIPGVLVGAALVGVLESLAAGYLDPLVGGGFSSIASYLVLVAVLFARPYG
                     LFGRPQVERV"
     gene            1647..2705
                     /locus_tag="VFX87_04055"
     CDS             1647..2705
                     /locus_tag="VFX87_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994114.1"
                     /translation="MTTDQSRRRLAAGLALSAALLAPVTAQAEIVLPAGFTARIYVTG
                     DGFGGSNEFRGRGIPTTPTLAVDHTGALYLARSGRRYSGGEFDYLTSIYRIRPGGAQL
                     SAATEARYFHGPPLNNAQVSGARGGREIFVTTFDRDRRVGVLYRLIDGRIVFFAGGTP
                     DPGTPPLLIQPEGAAGDSAGNVYVADRARGVVVRFDSTGRVLDPSYLRVLRPRTIAVD
                     DADHLWVGSDGSAEAPWQAGPGELWRVAPGGERRLMVEGPVSQGLSPGPGGSVFVADR
                     QANEIFGVTADGNRLSLARFTDGDAPRGLVFVPDTAETRAAGLAGDLLVSVIRIGVFQ
                     LNQIVRISGPFSDLARRR"
     gene            2726..3904
                     /locus_tag="VFX87_04060"
     CDS             2726..3904
                     /locus_tag="VFX87_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994115.1"
                     /translation="MNFGLSGPEQAFADDVRAFLHAHPPETFPEDGTDAGYGSGAHSR
                     AFTRALGERGWLSMGWPREHGGQARPMIYLLILLEELAAAGAPFGPLSGCLQVSDSII
                     RNGSETVQREVLPLVARGEATFWQGYSEPDAGSDLLALKTRARLEGDHWVLDGRKIWS
                     SHAGIATYGTVLARTDPASTRNRGLTMLMVDNRLPGMDVRPIRSLTGRVYHYEIFMDG
                     VRVPRDWVLGKPGEGFSQLLKGLDTDRFWGRFYKAPFLRRILGLLVEYVNAAMPGDAV
                     ARRRLAHAATEIAALRALFLRAAWLIERGLPATGPVSAAKVMADELGQRVLSMGMELL
                     GPYGVLRAGSPWARLRGEIEHQYQTSWGHTLAGGTSEIQRTTIAVRALGLPAEPRARA
                     "
     gene            3904..>4473
                     /locus_tag="VFX87_04065"
     CDS             3904..>4473
                     /locus_tag="VFX87_04065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994116.1"
                     /translation="MDLSFSPSQQLLQQSARSLLQQRCPPERVQELALDPRGFAADLW
                     KEIATLGWPGLLVPTELGGSGGSVADALALVEELGRACFPSPFIASAVIATTALTAAG
                     GRRARTLLPGLALGERICALAVLEESGRLDPDALTLPVTTPGRVTGRKLFVSDAHVAT
                     DVIVLGRAAAGPTALLLPMGRAGITARPLD"
BASE COUNT          523 a         1708 c         1541 g          701 t
ORIGIN      
        1 gcgcgatctc gaccccatcg cccgcgccgg accgggtgac gtccgcaaat cgctgctcca
       61 cctgcagtgc cacttcggga tggacaccat ccgctgggcc cagcagggcg cgatggtcac
      121 cggcgtggac ttctcggagg atgcggtgat cgccgcgcgc gccctcgcgg cccgcatggg
      181 ggtctccgcc accttcgtgc actgcgatct ctacgagctg cccgcgaagc tcgagggtcg
      241 cttcgacgtc gtcttcacct cccacggagt gctctgctgg ctcccggacc tcgagcggtg
      301 ggcccagctg atcgcgcgct tcgtggcgcc gggcggcctc ttctacatcg tggaggccca
      361 ccccttcctg cagatcttca acgatcagct cgccgagccg gacctgcgac tgctctaccc
      421 ctacttccac gggcccgagc cgttccgcta cgagcacccc ggctgcttca gcacccccga
      481 cgcgccgatc acgagcgtcg agcacgtctg gctgcacacc ctgtcggacg tcatcggcgc
      541 gctcgcgcgg gcggggctcc gcatcaccgc gttcgaggag tacccgttcc tcgcctggcg
      601 cttcttcccg tggatggagc agggccccga cggctggtgg cggctgccgg aggacccgcg
      661 gctgcccctc ggacgcggca gcctgccgct catgttctcg ctgaaggcga cgccgtgacg
      721 tcctgaatgc tcttcttctg gtcgctggtc gtggacggcg tgctggccgg cgccatctac
      781 gccttgatcg cgctgggctt cgtggtcgtc tacaaggcgt ccggcgcgat caatttcgcc
      841 ctgggcgaat gggcgctgct cggatcgcgg atgacggcga ccggggtcca cgcggtgggg
      901 ctcgggctga ccggagccat tgcctcggca tcggtcggca tggtcgcggt ggctctcgct
      961 ttcaaccgac tcgtgctgcg tccaatggtc ggccggccat tgctctccgt catcatggtc
     1021 accctcggtt ccggggtcct catccgcggg ctgaccccgt tggcgttcgg cgccatgcct
     1081 gccgccatcc cgttcccagc tccgccggcg ccgctcgaga tgtggggggt accggtcgcg
     1141 gccgagcggc tcgcttccgc cgtgatcgcg gttctctgca tcgcgctcgt gggcgggctc
     1201 tttcgtttca cccgaaccgg actcgcgctt cgggccatcg cggatgaccg acagatcgcg
     1261 atggcgatgg gcatcgacgt ccatcgacac gtctcgctcg cctgggcgat gctcggggcg
     1321 atttccgtcg tcaccggggc tctgtggacc atcacgtccg gcggggcgtt cggagtcgtg
     1381 atcgtcgggc tcaaggtgtt cccgatcctg atcatcggcg gcctcgacag catcccgggc
     1441 gtcttggtcg gcgccgctct cgtgggggtg ctggagagcc tggccgcggg ctatctcgat
     1501 cccctcgtgg gcggcgggtt cagcagcatc gcgtcctatc tggtgctcgt cgcggtgctg
     1561 ttcgcgcgac catacggcct gttcggccgg ccgcaggtcg agcgcgtgta gcgggatggc
     1621 ccggggaacg ctatcatgcg gactcaatga ccacggatca atctcgacgg cgccttgccg
     1681 ccggactggc cctgtcggcc gccctcctcg cgcccgtgac ggcgcaggcc gagatcgtcc
     1741 tgcccgccgg cttcaccgcc cggatctacg tcaccggcga cggcttcggc gggtccaacg
     1801 agttccgcgg gcgcgggatc cccacgacac ccacgctggc cgtggaccac accggcgcgc
     1861 tctatctcgc gcgcagcggc cggcggtaca gcggcggtga gttcgactac ctgacgtcca
     1921 tctaccgcat caggccgggc ggcgcgcagc tcagcgccgc gaccgaggcg cgctacttcc
     1981 acggcccgcc gctgaacaac gcgcaggtga gcggcgcccg cggcgggcgc gagatcttcg
     2041 tcaccacgtt cgaccgcgac cgccgcgtgg gcgtgctgta ccgcctcatc gacggccgga
     2101 tcgtcttctt cgcggggggt acaccggacc ccggcacgcc gccgctcctg atccagccgg
     2161 agggcgcggc cggggattcc gcgggcaacg tctacgtggc cgaccgcgcc cgcggcgtgg
     2221 tcgtgcgctt cgattcgacc gggcgcgtgc tggacccgag ctacctgcgc gtgctccgcc
     2281 cgcgcacgat cgcggtggac gacgccgatc acctgtgggt gggcagcgac ggcagcgcgg
     2341 aggccccgtg gcaggccggc cccggagagc tctggcgggt ggccccgggc ggcgagcggc
     2401 ggctcatggt ggagggcccg gtgtcgcagg gcctgagccc ggggccgggg ggctccgtct
     2461 tcgtggccga ccgccaggcc aacgagatct tcggcgtcac cgccgacggc aaccggctga
     2521 gcctcgcgcg cttcaccgac ggcgacgcgc cgcgggggct cgtcttcgtg cccgacaccg
     2581 ccgagacgcg cgccgccggt ctggccggcg acctgctcgt ctcggtcatc cgcatcggcg
     2641 tgttccagct caaccagatc gtgcggatct cgggcccctt ctcggacctg gcgcggcggc
     2701 ggtagtccca gggcaggccg cggccatgaa cttcgggctc tccggccccg agcaggcctt
     2761 cgccgacgac gtgcgggcct ttctccacgc ccatcctccg gagacgttcc ccgaggacgg
     2821 caccgacgcg ggctacggct ccggcgccca ctcgcgcgcc ttcacccgcg cgctcggcga
     2881 gcgcggctgg ctcagcatgg gctggccgcg cgagcacggc ggccaggcgc gaccgatgat
     2941 ctacctcctg atcctcctcg aagagctggc cgcggccggc gcgcccttcg gcccgctctc
     3001 cggctgcctg caggtctccg actccatcat ccgcaacggc tcggagaccg tgcagcgcga
     3061 ggtcctgccg ctcgtcgcgc gcggcgaggc cacgttctgg cagggctaca gcgagcccga
     3121 cgcgggctcg gatctcctcg cgctcaagac gcgcgcccgc ctcgagggcg atcactgggt
     3181 gctcgacggc cgcaagatct ggtcgagcca cgcgggcatc gcgacgtacg gcaccgtgct
     3241 ggcccgcacc gatcccgcat ccacccgcaa ccgcggcctc accatgttga tggtggacaa
     3301 ccggctgccc ggcatggacg tccgcccgat ccgcagcctg accggccgcg tctaccacta
     3361 cgagatcttc atggacggcg tgcgcgtgcc gcgcgactgg gtgctcggca agccgggcga
     3421 gggcttcagc cagctcctga agggcctcga caccgaccgc ttctgggggc ggttctacaa
     3481 ggcgccgttc ctgcgacgca tcctgggcct gctcgtggag tacgtgaacg ccgccatgcc
     3541 cggcgacgcg gtggcgcgcc ggcggctcgc ccacgccgcc accgagatcg cggccctccg
     3601 cgccctcttc ctccgcgcgg cctggctcat cgagcgcggc ctgcccgcga ccggcccagt
     3661 ctcggccgcg aaggtcatgg ccgacgagct gggccagcgc gtgctctcga tgggcatgga
     3721 gctgctcggc ccctacggcg tgctgcgcgc cggctccccg tgggcccggc tccgcggcga
     3781 gatcgagcac cagtaccaga cgagctgggg ccacaccctc gcgggcggga cctcggagat
     3841 ccagcgcacg accatcgcgg tgcgcgccct cggcctgccc gccgagcccc gggcccgcgc
     3901 ctgatggatc tctcgttctc gccgtcgcag cagctcctcc agcagtccgc gcgctcgctc
     3961 ctgcagcagc gctgcccgcc cgagcgcgtc caggagctgg ccctcgaccc gcgcggcttc
     4021 gcggccgatc tctggaagga gatcgcgacg ctcggctggc cgggcctgct ggtcccgacc
     4081 gagctgggcg gcagcggcgg ctccgtcgcc gacgcgctgg ccctcgtcga ggagctgggc
     4141 cgcgcgtgct tcccgagccc attcatcgcg agcgccgtca tcgccaccac cgcgctgacc
     4201 gcggccggcg ggcggcgcgc ccggaccctg ctccccggtc tggccctggg cgagcggatc
     4261 tgcgcgctcg ccgtgctcga ggagtcgggg cgcctcgacc cggatgccct gaccctgccc
     4321 gtcaccacac ccggccgcgt caccggacgc aagctcttcg tctcggacgc gcacgtggca
     4381 accgacgtga tcgtcctcgg ccgggccgcc gccgggccga ccgcgctcct gctgccgatg
     4441 ggccgcgccg gcatcaccgc gcgcccgctg gac
//
LOCUS       DASUFA010000109         5055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_598982, whole genome shotgun sequence.
ACCESSION   DASUFA010000109 DASUFA010000000
VERSION     DASUFA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598982"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFX87_04070"
     CDS             <1..492
                     /locus_tag="VFX87_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4994117.1"
                     /translation="FKNGITTAEISLDQVMGEALVVDLSWVGANHSITIDDLKKGGAG
                     DVKRGDIVLLHTGWTDKMYGRWPDYFTQSPYFPPEVAQWLVDRGPKNIGFDFFEEYCA
                     RLPEFTSEDFAMHRVILGAGVVIMEGLTNLGALPRRRVEFAAPFYKIAGTEGAPARFF
                     ATV"
     gene            489..1244
                     /locus_tag="VFX87_04075"
     CDS             489..1244
                     /locus_tag="VFX87_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994118.1"
                     /translation="MSGPDGVLLRTENLTRSFGSLVAVNGVSLTVRRGELRSIIGPNG
                     AGKTTFFRLVSGEMAPSSGRVLFQESDITGLPQHRVCRLGIAKSYQITNIFPHLSVLE
                     NVRVAVQGYARSFNFWSRADALADCRARAAGILATVSLARQAERLAAHLSHGEKRHLE
                     IGIALASDPVLLLLDEPTAGMSPEETDETMVLIRELARARTVVLVEHKMKLVMGISDR
                     VTVLHQGQVLADGTPAEIRANRVVQETYLGASR"
     gene            1241..1948
                     /locus_tag="VFX87_04080"
     CDS             1241..1948
                     /locus_tag="VFX87_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994119.1"
                     /translation="MSLLTVDDIHTYYGEAHILQGVSLTVGEGEVVTMIGRNGAGKTT
                     TLLSIMGIARARRGAVRLGGADITQLQTHEIVRRGIGWVPEERRVLPNLTVLENLRLG
                     MMATDGAGSEQRLEEALEFFPRLRERIAQKGRFLSGGEQQMLAIARGLVARPKIMLVD
                     EPTEGLAPLLVQSLTDILREINRRGTTILLVEQTLEVAMALSHRLYVMDQGRIQFEGT
                     PDTLRKDPTIQQRFLQL"
     gene            2086..3003
                     /locus_tag="VFX87_04085"
     CDS             2086..3003
                     /locus_tag="VFX87_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4994120.1"
                     /translation="MTRREWALLAVLAILWGVSFFFSKVALAELPPFTLVLGRFGIAA
                     LALLLAARLGGHRIPRSPRVWAGFFVLGALNSLIPFGLIAWGQVQLTSGLASILNATT
                     PLFTALVAHAWGDERLTSNRAAGVLIGLAGVCVLIGPGALVHLGAHTLAELAILGAAV
                     SYAFAGTYGRRFRALPPVLAVAGMMTTAALMALPVALVVDRPWTLDVGARTWGALLGL
                     ALLSTALGFVIYFRVLATAGATNVMLVTLLMPIVALLAGSLILSEPVAATALAGMALI
                     CAGLLAIDGRLLRARRAAVAPPSARSKAA"
     gene            3000..4211
                     /locus_tag="VFX87_04090"
     CDS             3000..4211
                     /locus_tag="VFX87_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994121.1"
                     /translation="MITTSSFPRLAWSNLAAQSAEQIGLAAAPLVAVLALGAGAGETG
                     FLQTAATLPFLLLAIPAGVLADRMPRRRLMAAAEAVRATALVATLVLASTGRLSLALL
                     AVLAFVGACGTVAYSVAAPALVPGLVPPAGLAAANARLELARTVAFVGGPALGGALVG
                     WTGAGAAFGVAAGLSIAAVFLLAGLREPPRAPAPPRRTLEDLREGSSFVFRHRLLLPI
                     LITQFVFNTAFFVLQAVYVPYAVHRLGLPASGVGVTLAVYGVGMVVGALLAGRLIAAL
                     PFGVVIVIGPLFGLAAALVMVSTIWMPVPALAGLSFFLIGVGPILWTIGTTTLRQMVT
                     PSLLLGRVSAINIATYGSRPLGAAVGALVGGLYGAETGLVVAAAGFLIQAVVILASPV
                     RRLARHSEMVG"
     gene            complement(4208..>5055)
                     /locus_tag="VFX87_04095"
     CDS             complement(4208..>5055)
                     /locus_tag="VFX87_04095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994122.1"
                     /translation="AVVTVPLYDPIRVVENMAFVDILSGGRIVLGLGSGYRPYEFEGF
                     GRDFDSRRDIQEEAIDLILELLHKRRTRHEGPHFHSVIEGDYEIFPVSQQDPHPPLFM
                     AGATDRSLGYAARHGFGLMLSTLPSPETLAKQIGLYRSQLKDAPAPLDRNPGFGKVDI
                     ARWVYVAETDAAARRDTEQGIVRHISHFMGAGTAGYLGNVSEKNRVEGLTYDELADTT
                     LLHGSPATVIAKLRELRHKTGLTSLLLHYPPYYGHDNAMRSLRLFAEKVMPEFRPPAS
                     RSAVA"
BASE COUNT          601 a         1788 c         1831 g          835 t
ORIGIN      
        1 ttcaagaacg gcatcaccac cgccgagatc tcgctggacc aggtgatggg cgaggcgctg
       61 gtggtggatc tctcctgggt cggcgccaac cactcgatca ccatcgacga tctgaagaag
      121 ggcggggcgg gcgacgtcaa gcgcggggac atcgtgctgc tgcacaccgg ctggaccgac
      181 aagatgtacg ggcgctggcc ggactacttc acccagtcgc cgtatttccc tcccgaggtc
      241 gcgcagtggc tggtggaccg cgggcccaag aacatcgggt tcgatttctt cgaggagtac
      301 tgcgcgcggc tgcccgagtt cacctcggag gacttcgcga tgcaccgggt gatcctgggc
      361 gcgggcgtgg tcatcatgga ggggctcacc aacctgggcg cgctgccccg gcgccgggtg
      421 gagtttgccg cgcccttcta caagatcgcc ggcaccgagg gggcgccggc gcgcttcttc
      481 gccaccgtat gagcgggccc gacggggtcc tgctgcggac ggagaacctc acccgctcct
      541 tcgggagcct ggtggcggtc aacggcgtct cgctcaccgt ccgacgcggg gagctccgct
      601 ccatcattgg gcccaacggg gcgggcaaga ccacgttctt ccgcctcgtg tcgggcgaga
      661 tggcgccgtc ctccgggcgc gtgctcttcc aggagtcgga catcaccggg ctgccgcagc
      721 accgcgtgtg ccgcctcggc atcgccaagt cgtaccagat cacgaatatc ttcccgcatc
      781 tgtcggtgct cgagaacgtg cgggtggccg tgcagggcta cgcgcgctcg ttcaacttct
      841 ggtcgcgcgc cgacgccctg gcggactgcc gggcccgggc cgccgggatc ctcgccaccg
      901 tcagtctcgc ccgccaggcc gagcggctcg ccgcgcatct ctcccacggc gagaagcggc
      961 acctcgagat cgggatcgcg ctcgcctccg atccggtgct gctgctgctc gacgagccga
     1021 ccgcgggcat gagcccggag gagaccgacg agacgatggt gctcatccgc gagctggccc
     1081 gcgcccgcac ggtggtgctg gtcgagcaca agatgaagct ggtgatgggc atctccgacc
     1141 gcgtcaccgt gctgcaccag ggccaggtgc tggccgacgg gacgcccgcg gagatccggg
     1201 ccaaccgcgt cgtgcaggag acctacctgg gagcctcgcg atgagtctgc tgacggtgga
     1261 cgacatccac acctactacg gcgaggccca catcctccag ggtgtgtcgc tcaccgtggg
     1321 cgagggcgag gtcgtgacga tgatcgggcg gaacggcgcg ggcaagacca ccacgctgct
     1381 gtccatcatg ggcatcgcgc gggcccggcg cggggcggtg cgcctgggcg gcgccgacat
     1441 cacccagctc cagacccacg agatcgtccg gcgcggcatc ggctgggtgc cggaggagcg
     1501 gcgcgtgctg ccgaacctga ccgtgctcga gaacctgcgg ctgggcatga tggcgaccga
     1561 cggcgccggc tccgagcagc gcctggagga ggcgctcgag ttcttcccgc ggctgcgcga
     1621 gcgcatcgcg cagaagggcc gcttcctctc gggcggcgag cagcagatgc tggccatcgc
     1681 ccgcggcctg gtggcgcggc ccaagatcat gctggtggac gagcccacgg aagggctcgc
     1741 cccgctgctg gtgcagagcc tcaccgacat cctgcgcgag atcaaccggc gcggcaccac
     1801 catcttgctg gtggagcaga cgctcgaggt cgcgatggcc ctctcccatc gcctctacgt
     1861 gatggaccag ggccgcatcc agttcgaggg caccccggac accttgcgca aggacccgac
     1921 gatccagcag cgcttcctgc agctctagtt cccctcaccc cgaccctcgt ccctctgggg
     1981 agagggggat gcgcgcgaga cgaccctctc ccctcagggg agagggctgg gtgaggggtg
     2041 gtccggttcc acccgttttg ctccgcgccc cgtataccgt tcgagatgac ccgacgagag
     2101 tgggcgctgc tggccgtgct ggctatcctg tggggcgtgt ccttcttctt cagcaaggtc
     2161 gcgctggccg agctgccccc gttcacgctc gtgctgggcc gcttcgggat cgccgcgctg
     2221 gcgctgctgc tggccgcgcg cctgggcggg caccgtatcc cgcgctcgcc gcgcgtgtgg
     2281 gccggcttct tcgtcctggg cgcgctcaat agcctcatcc ccttcggcct catcgcctgg
     2341 gggcaggtgc agctcaccag cggcctcgcc tcgatcctga acgcgaccac cccgctcttc
     2401 accgcgctgg tcgcgcacgc ctggggcgac gagcggctca cgtccaatcg cgccgccggt
     2461 gttctgatag gcctcgcggg agtgtgtgtg ctgatcggcc ccggggcgct cgtgcatctg
     2521 ggcgcccaca cgctggccga gctggcgatc ctgggcgcgg ccgtctccta cgccttcgcg
     2581 ggcacctatg gccggcggtt ccgcgcgctg ccgccggtgc tggcggtggc cggcatgatg
     2641 acgaccgccg cgctcatggc gctgccggtc gcgctggtgg tggaccggcc gtggacgctc
     2701 gacgtgggcg cgcggacgtg gggcgccctg ctcgggctgg cgctgctcag caccgcgctc
     2761 gggttcgtca tctacttccg cgtgctggcg acggccggtg ccaccaacgt catgctcgtg
     2821 accctgctca tgccgatcgt cgcgctcctg gccggctcgc tgatcctgag cgagccagtg
     2881 gccgcgacgg cgctcgcggg catggccctc atctgcgccg gcctgctcgc gatcgacggc
     2941 cgcctgctgc gcgcccggcg cgctgccgtc gccccgcctt ccgctcgctc gaaggcggcg
     3001 tgatcacgac ctcgagcttc ccgcgcctgg cgtggtcgaa tctggcggcg cagtcggccg
     3061 agcagatcgg cctggccgcg gcgcccctcg tggcggtgct cgcgctcggc gccggggcgg
     3121 gggagaccgg gttcctccag accgcggcga ccttgccgtt cctgctcctc gccattcccg
     3181 cgggcgtgct ggccgatcgc atgccgcgcc ggcggctcat ggccgccgcg gaggcggtgc
     3241 gcgccacggc gctcgtcgcc acgctcgtgc tggcgtcaac cggccggctg agcctggcgc
     3301 tgctcgcggt cctggctttc gtgggggcgt gtggcacggt ggcctacagc gtggccgcgc
     3361 ccgcgctcgt gccgggcctg gtgccgcccg ccgggctcgc ggcggcgaac gcgcggctcg
     3421 agctggcccg gaccgtggcc ttcgtgggcg gcccggcgct gggcggcgcg ctggtgggct
     3481 ggacgggtgc cggtgccgcc ttcggcgtcg ccgccgggct ctcgatcgcg gcggtgttcc
     3541 tgcttgccgg gctgcgggag cctccgcgtg cccccgcgcc cccgcgccgc accctggagg
     3601 atctccgcga gggctcgagc ttcgtgtttc gccatcggct gctgctgccg atcctgatca
     3661 cccagttcgt gttcaacacc gcgttcttcg tgctgcaggc ggtctatgtg ccctacgcgg
     3721 tccaccggct cggcctgccc gcctccggcg tgggcgtcac gctggccgtc tacggcgtcg
     3781 ggatggtggt gggcgcgctg ctggcgggcc gcctcatcgc cgcgctgccg ttcggcgtcg
     3841 tgatcgtgat cggcccgctc ttcgggctgg ccgccgcgct cgtgatggta tccacgatct
     3901 ggatgccggt gcccgcgctg gccgggctca gcttcttcct gatcggcgtg ggcccgatcc
     3961 tgtggaccat cggcacgacc acgctccggc agatggtgac accttcgctc ttgctcggcc
     4021 gcgtgtcggc catcaacatc gcgacgtacg gctcgcggcc gctgggcgcg gcggtcggcg
     4081 ccctggtcgg cgggctctac ggcgcggaga ccggcctggt ggtcgcggcg gcgggcttcc
     4141 tgatccaggc cgtcgtgatc ctggcctcgc cggtccgccg cctcgcgcgg cactcggaga
     4201 tggtcggcta ggcgacggcg ctccggctcg cgggcgggcg gaactccggc atcaccttct
     4261 cggcgaagag ccgcaggctc cgcatcgcgt tgtcgtgccc gtagtagggc gggtagtgga
     4321 gcagcagcga ggtgagcccg gtcttgtgcc gcagctcgcg cagcttggcg atcacggtcg
     4381 cgggcgagcc gtgcagcagg gtggtgtcgg ccagctcgtc gtaggtgagc ccctccacgc
     4441 ggttcttctc cgagacattg ccgaggtagc ccgcggtgcc cgcgcccatg aagtggctga
     4501 tgtggcggac gatgccctgc tcggtgtcgc gcctggccgc tgcgtcggtc tcggccacat
     4561 agacccagcg ggcgatatcc accttgccga agccggggtt gcggtcgagt ggggccggcg
     4621 cgtctttcag ctggctccgg tagaggccga tctgcttggc cagcgtctcc ggcgacggca
     4681 gggtggagag catcaggccg aatccgtgcc gcgccgcgta gccgagcgac cggtcggtgg
     4741 cgccggccat gaagagcggc gggtgcgggt cctgctggct caccgggaag atctcgtagt
     4801 ccccctcgat caccgagtgg aagtgcgggc cctcgtgccg ggtccgccgc ttgtgcaaga
     4861 gctcgaggat gaggtcgatg gcctcttcct ggatatcgcg ccggctgtcg aaatcgcggc
     4921 cgaagccctc gaactcgtac gggcggtagc ccgagcccag gcccaggacg atgcgccctc
     4981 ccgagaggat gtccacgaag gccatgttct cgacgacgcg gatggggtcg tagaggggca
     5041 cggtcaccac cgcgg
//
LOCUS       DASUFA010000110         7672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_713036, whole genome shotgun sequence.
ACCESSION   DASUFA010000110 DASUFA010000000
VERSION     DASUFA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713036"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFX87_04100"
     CDS             <1..516
                     /locus_tag="VFX87_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4994123.1"
                     /translation="SFLTADSGNTRDQTSTVYEAQIPRGYLGWGNVSTLGFSLAGAVA
                     AKLAYPQRQCVLVTGDAGVCYMMGNFEAVARYKIGITTIHINNGGYSGYGPGFWGEGH
                     DPYTWKVSDHGNACMASMARAVGFHGEDVTQPAEIIPALKRAFDENAKGRPAFVEFIC
                     SHHPVHGGWVR"
     gene            516..2006
                     /locus_tag="VFX87_04105"
     CDS             516..2006
                     /locus_tag="VFX87_04105"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4994124.1"
                     /translation="MAPSGIADIDPFASATTLLSALRRGVIGSAELTELYIRRIERHD
                     GRLNAVVVRDFDRARQQAKAADQSRGGNEPKPLLGLPITLKESINVTGLGTTCGVPDW
                     KGYVSPHDAPATARLRGAGAVLLGKTNVPPMLADWQSANPIFGRTSNPWDLGRTPGGS
                     SGGSAAALASGLTALEVGSDIGGSIRVPAVFCGVYGHRPSETLLPRSGQFPMPPMPNP
                     AVVMGVQGPLARSAEDLELALSVLAGPDVGEDVAWTVALPPARREKLADFRVAVLPPI
                     PWHPVDEQVWAAQQDLVARLGKLGCLVKEAQPDGLDNHREYHAFFRTLLAAVTGARMD
                     AETRQQRAAMFRRVGDDLSVAHARGFEGSAGDFILWHGRREQHRAAWRAFFRNWDVLL
                     APAINVLAYPHVERAWPMDDSDTTITLTVNGRPQPYLHSLCYPALSNVPGQPATAFPV
                     GISREGLPIGLQAIGPYLEDLTPIRFTALLAREIGGFRKPAGYEES"
     gene            2012..3172
                     /locus_tag="VFX87_04110"
     CDS             2012..3172
                     /locus_tag="VFX87_04110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4994125.1"
                     /translation="MLTIDGHCDPRFTAVREQFFANFTERGDVGAAVCVYQDGVRVVD
                     CWGGHADAARARPFGPDTIVSVASTTKGLVALCAHMLAERGKLDLDAPVARYWPEFAQ
                     AGKEDIPVRWLLSHRAGLPAIRRSLSAAALFDWTAMTAAIAETAPWWTPGERHGYHAI
                     TYGHLVGEVIRRADGRSVGAFLRDEVTGPLRADFFIGVPAEADGRAAEVLPPPPAGEP
                     TIWDTLLADPESVSGRTFLNPPRTPDLVNTRAWRAAEIPAANGHTSARGVARVYAALA
                     RGGELDGVRLLAPATIERAIEEQSRGRDEVLTLPTRFATGFMLGMPGGIFECGPGRRT
                     FGHPGRGGSIGFADPDARIGLGYVTNQYVTGTAKHPDRRVLHLVDAVYAALR"
     gene            3169..4359
                     /locus_tag="VFX87_04115"
     CDS             3169..4359
                     /locus_tag="VFX87_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4994126.1"
                     /translation="MSPAPALSARDCLPRDAGDALLVGRAWVPAEDGPCVVAVRGDEV
                     VDLTRTYPTTSHLLNIARPADVRAAIKVAPRLATLEALAANSVEGARDASKPWLLAPA
                     DLQAVKASGVTFVASMLERVIEERAKGNPALADGARREIQAIVGPDLRGIKPGSPEAA
                     RIKEVLVAKGMWSQYLEVGIGPDAELFSKCPPMAAVGPGARVGVHPASVWSNPEPELV
                     LVINREGAIVAVTLGNDVNLRDVEGRSALLLGRAKDNNASCAMGPFFRLLDERFTLDH
                     ARRVTFETVVQGPDGFRISHLTQVAEISRDLTELAGQAIGRYHRYPDGLVLFIGTMFA
                     PVADRGAPGSGFTHKTGDNVSIHSPQIGRLVNEVVACDAAEPWAFGTGALMASLARRG
                     LLRA"
     gene            complement(4262..4912)
                     /locus_tag="VFX87_04120"
     CDS             complement(4262..4912)
                     /locus_tag="VFX87_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4994127.1"
                     /translation="MIKPIRRIVTGHNARGRSIFVSDAPSPHVLTLPGRPDFALTDLW
                     ASDGAPASNAGSADAAKRRMSLEPPRNGTIFRIVEFPPDAAGGGFDRAAAFRAMGASH
                     AMDPDASRHPAMHRTDTVDYAIVLSGEIWALMDEGETLMRAGDTLVQRGTNHAWSNRS
                     DQPCLVVFVLVSAAPLGGKGAATPVKRAAARAGRGSPSRRPSRRPTARPRRKPRPR"
     gene            5003..6685
                     /locus_tag="VFX87_04125"
     CDS             5003..6685
                     /locus_tag="VFX87_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994128.1"
                     /translation="MTDLVIRGGTVVDGSGKAGRRADVAIQDGRVGAVGEIRERGVRE
                     IDAGGLVVAPGFIDVHTHYDAQYTWDPYATSSIWHGVTTTVIGNCGFAIAPCRPADRD
                     LIMRTLVKVEGMSLAAMRTGITWGFETFPQYLDHLERCNPSLNVAALLGHSTIRQWVM
                     GGDSQHRVATSEEVAAMCGLVREAMAAGAIGIGSSTAEAHVGEGGLPVASRLADKAEV
                     LALTKAMGESGRGLFQATIGPKTTMEDLREIWRTSGRPVIWAAFFQRDDRPEYVPERL
                     AITEAFHREGVEVLPQISCRPLTMDFTMKNPYPFEGMPAWQQVMKQPESRWLSVYGDP
                     GFRTALKADLDARRLAVFRGRWDLVQVLRTNRPEHKALEGRSLADLARETGKHPVDAW
                     LDLVTADGLDVEFLAGLLNTDEKVVGDLIVHPLTLITLSDAGAHLSLMCDAGYSSTLL
                     GKWVRALKRLPLEAAIKRLSDDPARAYRIPQRGRLEPGYWADVAIFDPATVDALPAEW
                     VTDLPADEPRFVSRARGVEWSLVNGQPVLERGRVVEQVPGKRPGQVLRRFDA"
     gene            complement(6701..>7672)
                     /locus_tag="VFX87_04130"
     CDS             complement(6701..>7672)
                     /locus_tag="VFX87_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994129.1"
                     /translation="VCEVVKAAAAAALALGALGGWQSRTSIFALVALLGAAQAFENPA
                     MSALVPEVVARSLIAPAMAWVISAGQTAQIVGPALGGLLYVFGPGAAYFTAAGLFILA
                     GALAAAIRVERVARRREPLTLETVFSGVAFIYGQRVLLGSMSLDLFAVLLGGATALLP
                     VFAKDILGVGPVGLGVLRSAPAVGALATSVFLAHYPLERRLGPTLFRSVTVFGAATVV
                     FGVSTNFVLSLVALTILGAADVVSMVIRMSLAQIRTPDAMRGRVSAVHSLFTGTSNQL
                     GAFESGVAAALLGTVPAVLLGGVGTIAVAGLWMLLFPELRRLGRFEE"
BASE COUNT         1089 a         2862 c         2724 g          997 t
ORIGIN      
        1 tcgttcctca ccgcggactc cggcaacacg cgcgaccaga ccagcaccgt gtacgaggcc
       61 cagatcccgc gcggctatct cgggtggggc aacgtctcca cgctcggctt cagcctggcc
      121 ggcgcggtgg cggccaagct cgcctacccg cagcggcagt gcgtgctcgt gacgggtgac
      181 gcgggcgtct gctacatgat gggcaacttc gaggcggtgg cgcggtacaa gatcgggatc
      241 acgacgatcc acatcaacaa cggcggctac tcgggctacg gccccggctt ctggggcgag
      301 ggccacgatc cctacacctg gaaggtatcg gaccacggca acgcgtgcat ggcgagcatg
      361 gcccgcgcgg tcggcttcca cggcgaggac gtgacccagc ccgccgagat catccccgcg
      421 ctcaagcgcg ccttcgacga gaacgccaag ggccgccccg cgttcgtgga gttcatctgc
      481 tcgcaccacc cggtgcacgg gggctgggtg cgctgatggc cccgagcggg atcgccgaca
      541 tcgacccgtt cgcgagcgcg accacgctcc tgtccgcgct gcgccggggc gtgatcggct
      601 cggcggagct gaccgagctg tacatccgcc gtatcgagcg gcacgacggc cggctcaacg
      661 cggtggtcgt ccgcgacttc gaccgcgccc gccagcaggc gaaggccgcc gatcagagcc
      721 ggggcggcaa cgagccgaag cccctcctcg ggctgccgat cacgctcaag gaatccatca
      781 acgtcaccgg gctcgggacg acctgcggcg tcccggactg gaagggctac gtctccccgc
      841 acgacgcccc cgccaccgcg cgcctccggg gggccggcgc cgtgctgctc ggcaagacca
      901 acgtgccgcc catgctcgcg gactggcagt cggccaaccc gatcttcggg cgcaccagca
      961 atccctggga cctgggccgg acgcccggcg ggagcagcgg gggcagcgcg gcggctctcg
     1021 cgtctggatt gaccgctctg gaagtcggct cggacatcgg cggctccatt cgcgtgcccg
     1081 ccgtcttctg cggcgtctac ggccatcggc cgagcgagac cctgctgccg cgcagcggcc
     1141 agttcccgat gccgccgatg cccaacccgg ccgtcgtgat gggcgtgcag ggtccgctcg
     1201 cgcgcagcgc ggaggatctc gagctggcgc tctccgtgct ggccggcccc gacgtgggcg
     1261 aggacgtggc gtggacggtc gcgctgccgc ctgcccgccg agagaagctg gccgacttcc
     1321 gcgtggcggt gctcccgccg atcccgtggc atccggtgga cgagcaggtc tgggccgccc
     1381 agcaggatct cgtggcccgc ctcggcaagc tgggctgcct cgtcaaggaa gcacagcccg
     1441 acggcctcga caaccaccgc gagtaccacg ccttcttccg caccctgctc gccgcggtca
     1501 ccggcgcgcg catggacgcg gagacgcggc agcagcgcgc cgccatgttc cgccgggtgg
     1561 gtgacgatct gtcggtcgcc cacgcgcgcg gcttcgaggg ctcggcgggc gatttcatcc
     1621 tctggcacgg ccggcgcgag cagcaccgcg cggcctggcg tgcattcttc cgcaactggg
     1681 acgtgctgct ggccccggcc atcaacgtgc tcgcctatcc gcacgtcgag cgggcgtggc
     1741 cgatggacga cagcgacacc acgatcaccc tcaccgtgaa cggccggccc cagccctatc
     1801 tccacagcct gtgctatccc gcgctgtcca acgtgccggg ccagcccgcc accgcgttcc
     1861 cggtgggtat ctcgcgcgag gggctgccga tcgggctcca ggccatcggg ccttatctgg
     1921 aagacctgac gccgatccgc ttcaccgcgc tgctcgcgcg ggagatcggc ggcttccgca
     1981 agcccgccgg ctacgaggaa tcctagaccc gatgctgacg atcgacgggc actgcgaccc
     2041 gcgcttcacc gccgtccgcg agcagttctt cgccaacttc acggagcgcg gggacgtggg
     2101 cgccgcggtg tgcgtgtacc aggacggcgt ccgcgtggtg gactgctggg gcggccacgc
     2161 cgacgccgcc cgcgcgcggc ccttcggccc ggacaccatc gtgagcgtgg cctccaccac
     2221 caagggcctg gtcgcgctct gcgcccacat gctggccgag cgcggcaagc tcgatctgga
     2281 cgcgccggtc gcgcgctact ggccggagtt cgcccaggcc ggcaaggagg acatcccggt
     2341 gcgctggctg ctgagccacc gcgcgggcct gcccgcgatc cggcggtccc tgtcggccgc
     2401 ggcgctcttc gactggaccg cgatgaccgc ggcgatcgcg gagaccgcgc cctggtggac
     2461 ccccggtgag cgccacggct atcacgcgat cacctacggc cacctggtcg gcgaggtcat
     2521 tcgccgcgcg gacggccgct ccgtgggcgc gtttcttcgc gacgaggtca cgggcccgct
     2581 gcgcgccgac ttcttcatcg gcgtgccggc ggaggccgac gggcgcgccg ccgaggtcct
     2641 accgccaccg ccggccggcg agccgacgat ctgggacacg ctcctcgcgg atcccgagtc
     2701 ggtctccggc cgcacgttcc tgaatccgcc gcgcacgccc gacctggtca acacccgcgc
     2761 gtggcgcgcc gccgagatcc cggcggccaa cggccacacc agcgcgcgcg gggtggcccg
     2821 cgtctacgcg gcgctggccc gcggcggcga gctcgacggc gtccgcctgc tcgcgcccgc
     2881 caccatcgag cgcgccatcg aggagcagtc ccgcggtcgc gacgaggtgc tgaccctgcc
     2941 cacgcgcttc gccaccggct tcatgctcgg gatgccgggc gggatcttcg agtgcggccc
     3001 cggccgccgc accttcggcc atccgggacg cggcggctcg atcggcttcg ccgacccgga
     3061 cgcgcgcatc gggctcggct acgtgaccaa ccagtacgtg accggcaccg cgaagcatcc
     3121 cgaccgccgc gtcctccacc tggtggacgc ggtgtacgcg gcactccgat gagcccggcg
     3181 cccgcgctct ccgcccgcga ctgcctgccc cgcgatgcgg gcgacgcgct gctggtcggc
     3241 cgcgcgtggg tccccgccga ggacggtccc tgcgtcgtcg cggtgcgcgg cgacgaggtg
     3301 gtggatctga ctcgcacgta tccgacgacg agccacctgt tgaacatcgc caggcccgcc
     3361 gacgtgcgtg ccgcgatcaa ggtcgcgccg cggctcgcga cgctggaagc cctggccgcc
     3421 aacagcgtgg agggcgcccg cgatgcctcg aagccgtggc tcctcgcccc cgcggacctg
     3481 caggcggtga aggccagcgg cgtgacgttc gtggcgagca tgctcgagcg cgtgatcgag
     3541 gagcgcgcca agggcaaccc ggccctggcc gacggggcgc ggcgcgagat ccaggccatc
     3601 gtgggacccg atctccgcgg catcaagccc ggctcgccgg aggccgcgcg catcaaggag
     3661 gtcctggtgg ccaaaggcat gtggtcccag tacctcgagg tcggcatcgg cccggacgcg
     3721 gagctcttca gcaagtgccc gccgatggcc gcggtggggc cgggcgcgcg cgtgggcgtg
     3781 cacccggcat cggtgtggag caaccccgag ccggagctgg tgctggtcat caatcgcgag
     3841 ggcgccatcg tggcggtcac cctcggcaac gacgtgaacc tgcgcgacgt ggaaggccgg
     3901 agcgcgctgc tgctgggccg cgccaaggac aacaacgcct cgtgcgcgat gggcccgttc
     3961 ttccggctgc tcgacgagcg gttcaccctg gaccacgcgc gccgggtcac cttcgaaacg
     4021 gtcgtgcagg gcccggacgg cttccggatc tcccacctga cccaggtcgc cgagatcagc
     4081 cgcgacctga ccgagctggc cggccaggcc atcggccggt accaccgcta cccggacggc
     4141 ctcgtgctct tcatcggcac catgttcgcg ccggtggccg accgcggcgc gccgggcagc
     4201 ggcttcaccc acaagaccgg cgacaacgtc agcattcact caccccagat cgggcgcctg
     4261 gtcaacgagg tcgtggcttg cgacgcggcc gagccgtggg ccttcgggac gggcgccttg
     4321 atggcgagcc tcgcccggcg cgggctgctg cgcgcttgac cggcgtggcg gcgcccttgc
     4381 cgccgagcgg tgccgcgctg accaggacga agacgaccag gcagggctga tcgctccggt
     4441 tgctccaggc gtgattggtg ccgcgctgta cgagggtgtc gccggcccgc atcagcgtct
     4501 cgccctcgtc catcagcgcc cagatctccc ccgacagcac gatggcgtag tccacggtgt
     4561 ccgtccggtg catggccgga tggcgcgagg cgtccgggtc catggcgtgg ctggcgccca
     4621 tcgcgcggaa ggccgccgcg cgatcgaagc cgccccccgc cgcgtcgggc gggaactcga
     4681 cgatccggaa gatggtgccg ttgcggggcg gctcgagcga catgcggcgc ttcgccgcgt
     4741 ccgcgctccc cgcgttggac gcgggcgccc cgtcgctcgc ccacagatcg gtgagcgcga
     4801 aatcggggcg ccccggcagg gtgagcacgt gcggcgacgg cgcgtcgctc acgaagatgg
     4861 agcggccccg ggcattgtga ccggtgacga tgcgtcggat cggcttgatc atggtggcct
     4921 cccggccggg actatacacc gccctccggc tgggtgaaac tgtgggcggc gcagggattc
     4981 cgcagggcga aagagagacg acatgaccga cctggtgatt cgcggcggca ccgtggtgga
     5041 cggcagcggc aaggcggggc ggcgggccga cgtggccatc caggacgggc gcgtcggcgc
     5101 ggtcggcgag atccgtgagc gcggcgtgcg cgagatcgac gcgggcggcc tcgtcgtcgc
     5161 ccccggcttc atcgatgtcc acacccacta cgacgcccag tacacgtggg acccctatgc
     5221 caccagcagc atctggcacg gcgtcaccac caccgtgatc ggcaactgcg gcttcgccat
     5281 cgcgccctgc cgccccgccg accgcgacct gatcatgcgc acgctcgtga aggtggaggg
     5341 catgtcgctg gccgccatgc gcaccggcat cacctggggc ttcgagacct tcccgcagta
     5401 cctcgatcac ctcgagcggt gcaacccgag cctcaacgtg gccgcgctgc tcggccactc
     5461 gacgatccgc cagtgggtga tgggcggcga cagccagcac cgcgtggcca cctccgagga
     5521 ggtggcggcg atgtgcggcc tcgtgcgcga ggcgatggcc gccggcgcga tcgggatcgg
     5581 ctcctccacc gccgaggcgc acgtgggcga gggcgggctg ccggtggcga gccggctcgc
     5641 ggacaaggcg gaggtcctcg cgctgacgaa ggccatgggc gagtccgggc gcggtctctt
     5701 ccaggcaacg atcggcccca agaccaccat ggaggacctg cgcgagatct ggcgcaccag
     5761 cggccgcccg gtgatctggg ccgcgttctt ccagcgcgac gaccggcccg agtacgtgcc
     5821 ggagcgcctc gccatcaccg aggcgttcca tcgcgagggc gtggaggtgc tgccgcagat
     5881 ctcctgccgg ccgctcacca tggacttcac gatgaagaac ccctacccgt tcgagggcat
     5941 gccggcctgg cagcaggtca tgaagcagcc cgaatcgcgg tggttgagcg tctacggtga
     6001 tcccggcttc cgcaccgcgc tcaaggccga tctcgacgcg cgccgcctcg cagttttccg
     6061 cgggcgctgg gacctcgtcc aggtgctgcg caccaaccgc cccgagcaca aggcgctcga
     6121 gggccggagc ctcgcggatc tcgcgcggga gaccggcaag catccggtgg acgcctggct
     6181 cgacctggtg accgccgacg gcctcgacgt ggagttcctg gccgggctgc tcaacaccga
     6241 cgagaaggtg gtgggcgatc tcatcgtgca cccgctcacc ctgatcacgc tctccgacgc
     6301 gggcgcgcat ctctccctga tgtgcgacgc cggctactcc agcacgctcc tcggcaaatg
     6361 ggtgcgcgcc ctcaagcgcc tgccgctcga ggcggcgatc aagcgcctca gcgacgaccc
     6421 cgcgcgcgcc taccgcatcc cgcagcgcgg ccgcctcgag cccggttact gggccgacgt
     6481 ggcgatcttc gacccggcca ccgtggacgc cctgcccgcc gagtgggtga ccgacctgcc
     6541 cgccgacgag ccgcgctttg tctcccgcgc gcgcggcgtg gagtggagcc tggtcaacgg
     6601 ccagccggtc ctcgagcgcg ggcgcgtggt ggagcaggtg cccggcaagc gcccgggcca
     6661 ggtgctgcgc cgcttcgacg cctgagcggc ggccgcgccg ctactcctcg aagcggccca
     6721 gccgacgcag ctcggggaag aggagcatcc acagcccggc caccgcgatc gtgccgacgc
     6781 cgcccagtag caccgcgggc acggtgccca gcagggccgc ggccaccccg gactcgaagg
     6841 cgccgagctg gttcgaggtg ccggtgaaga gcgagtgcac cgcgctcacc cgcccgcgca
     6901 tggcgtccgg cgtgcggatc tgggcgagcg acatgcggat gaccatgctg accacgtcgg
     6961 ccgcgcccag gatcgtgagc gccaccaggg acaggacgaa gttggtggag accccgaaga
     7021 ccacggtggc ggccccgaac acggtcaccg agcggaacag ggtcggcccc agacgccgct
     7081 ccagcgggta gtgcgccagg aacacggacg tggccagcgc gccgaccgcg ggcgccgatc
     7141 gcagcacccc gagcccgacc gggcccactc ccaggatgtc cttggcgaac acgggcagga
     7201 gcgcggtggc gccgcccagc agcaccgcga agagatcgag cgacatggag cccagcagga
     7261 cccgctggcc gtagatgaac gcgacgcccg agaacacggt ctcgagcgtg agcggctcgc
     7321 gccgccgcgc cacgcgctcg acccggatcg cggccgcgag cgcgcccgcc aggatgaaga
     7381 ggcccgcggc cgtgaagtag gcggcgccgg ggccgaagac gtagagcagc ccgcccaggg
     7441 ccggcccgac gatctgggcg gtctgccccg ccgagatcac ccacgccatc gcgggggcga
     7501 tgagcgagcg cgccaccacc tccggcacca gcgcgctcat cgccgggttc tcgaaggcct
     7561 gcgccgcgcc caggagcgcc accagcgcga agatgctggt ccggctctgc cagccgccga
     7621 gggcgcccaa cgctagcgcc gccgcggcgg cggccttcac gacctcgcac ac
//
LOCUS       DASUFA010000111         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1617044, whole genome shotgun sequence.
ACCESSION   DASUFA010000111 DASUFA010000000
VERSION     DASUFA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617044"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFX87_04135"
     CDS             <1..886
                     /locus_tag="VFX87_04135"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4994130.1"
                     /translation="VFNRGRHPERLPAGVAALTGDRKDHAALRERLGGGRWDGVYDVT
                     YAPTLPEDVAALADALPGSPHVFFVSTCRVYDHALPIPYSEETARSFYWGDYARHKIG
                     GEDVLLERYRERGLPVTIVRPTHVMGPLNTRNNEAFFMDRISRGRPVLVPGHGGWLRQ
                     FGHVEDLAEVMATLLGDSRAYGQAYNVMGEDVVSQVGFVELIAEVIGRPVTLRHFDAA
                     LLKSFDKPGPVFGQNLIYDCHAVHTTTKLRNALGLRPRYTLHAGLAQTWEWYRAQGLA
                     DRPVDFSFEDRLLAKIGA"
     gene            883..1827
                     /locus_tag="VFX87_04140"
     CDS             883..1827
                     /locus_tag="VFX87_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4994131.1"
                     /translation="MTRGILVYYPDAGEARAYADLIRLPSRAFRVHVASTPEEAAGPG
                     ALAEIFYCWGPPRPLLASAKGLRWIQCMGAGVERLLVPELPAGVRITRVAGIFGPWMA
                     EYTLGWCLWVTQRMEGFRASQRQRRWAPVDPVPLRGQTLCVVGLGDIGGHIAKLARAL
                     GMHVIGVTRSGRAARNAERVYRTNALRAALARADFVALTLPLSDKTLGLLGPAELAAM
                     KPTAWLLNLARGPIVDEAALLDALRGRRIGGAVLDVFDTEPLPADHPLWGFDNVVVTP
                     HISGPSTPREIGPIFNDNLRRYAANRPLRHTVNRTRQY"
     gene            complement(1863..2909)
                     /locus_tag="VFX87_04145"
     CDS             complement(1863..2909)
                     /locus_tag="VFX87_04145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994132.1"
                     /translation="MALPTVDAPCFLCGAPDADPLWSTPDRAFAVPGIYTVARCRTCG
                     FLYQRPRVRDEHLAACYPDDYPRHQEPSPRLPFKGSPARVRAVRWALASRLGYESLRE
                     TGAGLITRARAALVFRRIKWDCPPWRGQGRYLDVGCGSGGALGVAQALGWQVAGVEVD
                     EVAAKKARRFSTRVHAGDLIEAPFAPGEFDCVTAFHVLEHVPDPVAAVRRMLEWLAPG
                     GQLVIEVPNAGGLGAQLFGRAWSGLELPRHLSHFTPETLGAVVRQAGGRVVWTWHQAK
                     PRYYLWSLGFWLRDRGLGRLADAAAWPPVYGLLKLALELTLPLARWVGRGEVVRVGVE
                     HRALPGQPSPCPLP"
     gene            complement(2968..>3278)
                     /locus_tag="VFX87_04150"
     CDS             complement(2968..>3278)
                     /locus_tag="VFX87_04150"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4994133.1"
                     /translation="GFASPYMMLAMPTRPEARDALTAALHPQDATARPQILEREWNTE
                     YHAVIREFERRTGVGAVLNTSFNLHGEPIVGGAADAVDTFERSGLPHLAVGHWLLSKK
                     "
BASE COUNT          466 a         1227 c         1143 g          442 t
ORIGIN      
        1 cgtcttcaac cgcggccggc atcccgagcg gttgcccgcc ggggtcgccg cgctcaccgg
       61 cgaccgcaag gatcacgcgg cgctccgcga gcggctcggc ggcgggcggt gggacggggt
      121 ctacgacgtc acctacgcgc cgaccctgcc ggaggacgtg gccgcgctgg cggacgcgct
      181 cccgggctcg ccgcacgtct tcttcgtttc cacgtgccgg gtctacgacc acgccctccc
      241 catcccctac tccgaggaaa ccgcgcggag cttctactgg ggcgattacg cgcggcacaa
      301 gatcggcgga gaggacgtgc tcctcgagcg ttaccgggag cgcggcctgc ccgtgaccat
      361 cgtccgcccc acccacgtga tgggcccgct gaacacccgg aacaacgaag cgttcttcat
      421 ggaccggatc agccggggcc gcccggtgct ggtgccgggc cacgggggct ggctgcggca
      481 gttcggccac gtggaggacc tggccgaggt gatggccacg ctcctgggcg actcgcgggc
      541 ctacgggcag gcctacaacg tgatgggcga ggacgtggtg agccaggtgg gcttcgtcga
      601 gctgatcgcc gaggtgatcg gccggccggt cacgctccgg cacttcgacg ccgccctgct
      661 caagagcttc gacaagccgg gccccgtctt cggccagaac ctgatctacg actgccacgc
      721 cgtccacacc accaccaagc tgcgcaacgc gctgggtctc cggccccgct acacgctcca
      781 cgcggggctc gcccagacct gggagtggta ccgcgcccag ggcctggccg accggcccgt
      841 ggacttttcc ttcgaagacc ggctgctcgc caagatcggc gcgtgacccg cggcattctc
      901 gtgtactacc ccgacgcggg ggaagcccgc gcctacgccg acctcatccg tctgccgtcg
      961 cgcgccttcc gcgtccacgt ggcctcgacc cccgaggagg ccgccgggcc gggtgctctg
     1021 gccgagatct tctactgctg ggggccgccg cgccccctgc tcgcgagcgc gaagggactt
     1081 cgctggatcc agtgcatggg cgccggggtc gagcgcctgc tcgtgcccga gctgccggca
     1141 ggtgtccgca taacccgcgt cgcggggatc ttcggaccgt ggatggccga gtacacgctg
     1201 ggctggtgcc tctgggtcac gcagcgcatg gagggcttcc gcgcgagcca gcggcagcgg
     1261 cgatgggcgc cggtggatcc cgtgccgctg cgcggccaga ctctctgcgt cgtcggcctc
     1321 ggcgatatcg gcggccacat cgcgaagctg gcccgcgccc tcggcatgca cgtcatcggc
     1381 gtcacgcgga gcggccgggc cgcgcgcaac gccgagcggg tctaccggac gaacgcgctc
     1441 cgcgccgcgc tcgcccgcgc ggacttcgtg gccctgacgc tgccgctgag cgacaagacc
     1501 ctggggctcc tgggcccggc cgagctggcc gcgatgaagc ccaccgcctg gctactcaac
     1561 ctggcgcgcg ggcccatcgt ggacgaggcc gcgctgctgg acgcgctgcg cgggcgccga
     1621 atcggcggcg cggtgctcga cgtcttcgat accgagcccc tgcccgccga ccatccgctc
     1681 tgggggttcg acaacgtggt ggtcacaccg cacatctcgg gccccagcac cccgcgcgag
     1741 atcgggccca tcttcaacga caacctccgc cgctacgccg ccaaccgccc cctccgccac
     1801 accgtcaacc gcacccgcca atactagggg ccgtccttcc ccttccccat ccccctctcc
     1861 cctcagggga gagggcaggg tgagggctgg cctggtaagg cccggtgctc gacgcccacc
     1921 ctcaccacct ccccacgccc gacccaccga gctagcggca gtgtgagctc gagggcgagc
     1981 ttcagcagcc cgtacaccgg cggccacgcc gcggcgtccg ccagccttcc aagtccgcgg
     2041 tcgcgcagcc agaacccgag actccagagg tagtatcgcg gcttcgcctg atgccatgtc
     2101 catacgacac gacccccggc ctgccggacg accgcgccca gcgtctcggg cgtgaagtgc
     2161 gagagatgcc gcggcagctc gagcccggac caggctcggc cgaagagctg cgccccgagg
     2221 ccgcccgcgt tcggcacctc gatcacgagc tgcccgccgg gcgcgagcca ctcgagcatg
     2281 cgtcgcaccg cggcgaccgg gtccggcacg tgctcgagca cgtggaaggc cgtgacgcag
     2341 tcgaactcgc ccggcgcgaa cggggcctcg atcagatcgc cggcgtgcac gcgtgtgctg
     2401 aaacgccggg ccttcttcgc ggcgacttcg tccacctcaa cgccggcgac ttgccagccg
     2461 agggcctgcg cgacgcccag cgcgccgccc gagccgcagc ccacgtcgag gtagcggccc
     2521 tgcccccgcc agggcggaca gtcccacttg atgcgccgga atacgagggc cgcgcgagcc
     2581 cgcgtgatga ggcccgctcc ggtctcgcgc agcgactcgt agccgagcct cgaggcgagc
     2641 gcccagcgga ccgcgcgtac ccgcgcgggc gaacccttga acgggaggcg tggggacggc
     2701 tcctgatggc gagggtagtc atcgggatag caggcggcca ggtgctcgtc tcgcacccgc
     2761 ggccgctggt agaggaagcc gcacgtccgg cagcgcgcga ccgtgtaaat gccgggcacc
     2821 gcgaaggcgc ggtcgggcgt ggaccagagt gggtcggcgt cgggtgcccc gcaaagaaag
     2881 cagggggcgt ccacggtagg gagcgccatt gcgctcaccc ctcaccctgc cctctcccgt
     2941 gaggggagag ggtaaaggga tgcggcatta cttcttcgac agcagccagt gcccgaccgc
     3001 gaggtgtggc aggccggagc gctcgaaggt gtccaccgcg tccgcggcgc cgccgacgat
     3061 gggctcgccg tggaggttga acgaggtgtt gaggaccgcg cccacgccgg tccgccgctc
     3121 gaactcgcgg atcaccgcgt ggtactcggt gttccactcg cgctccagaa tctgcggccg
     3181 agcggtggcg tcctggggat ggagcgcggc ggtgagcgcg tcccgcgcct ccgggcgcgt
     3241 cggcatcgcg agcatcatgt agggcgaggc gaagccct
//
LOCUS       DASUFA010000112         8400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1515851, whole genome shotgun sequence.
ACCESSION   DASUFA010000112 DASUFA010000000
VERSION     DASUFA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515851"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="gltX"
                     /locus_tag="VFX87_04155"
     CDS             <1..979
                     /gene="gltX"
                     /locus_tag="VFX87_04155"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX4994134.1"
                     /translation="PREGETVVDDVNRGRVVFSHAELDDWILVRTDGTPTYNFCVVVD
                     DVTMKITHVIRGSDHLSNTPKQVLCYRALGYPLPVFAHIPMILAADKTRLSKRHGATS
                     VLAFRDMGILPEAMLNYLARLGWSHGDQEIFSREDLIRHFDLAHVGAADAVFDLEKFT
                     WVNHQWIKRLPAARLAADLVPFLARAGLPAPRDVDWLARVADTLKERARTLVEMAEQA
                     TFYLRPPDAYDPQSTAKFWKEQAAERYAILIKRLEAQEAMDPQALEELFRFLAADLGL
                     KLVDLAQLTRIALTGKTASPPIFEVVSILGKPETLARLKAAAAAVEARR"
     gene            976..1608
                     /locus_tag="VFX87_04160"
     CDS             976..1608
                     /locus_tag="VFX87_04160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4994135.1"
                     /translation="MRRLLLARHGQSLSNAVRRFQGAQDVALSPLGIRQAEALGLAVG
                     RRSIAHVYASPLERARHTALIALAGLDLPLTLVDDLRELSLGEWEGCTVEEIRTQPGD
                     PYARWVRDPVQCLPPGGEPLADVQARVLRAVAEIVSAHPDEETILIISHGGVISALLA
                     HWLGLPLSSIWRIAVANCSLSEIAPPRVISVNETGHLRDIDVVVAAPLSP"
     gene            1610..3217
                     /locus_tag="VFX87_04165"
     CDS             1610..3217
                     /locus_tag="VFX87_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX4994136.1"
                     /translation="MTLLALFGGLALLLYGMQLIGEGLQRAAGSHLRHLLTSMTRTRL
                     AAVASGALVTSIIQSSSATTLMLIGFVSAGLVTFPQSLGVILGADIGTTVTVQLLAFK
                     VQELSLLLVGVGFAMTFFARRGLVKSLGQVILGFGFIFLGMKVMNDGLAPLTENELTR
                     QVLVALADNQFLALLVGAVLSASMASSAATIGLTLSLGHQGLLPLGAAIPVVLGANIG
                     TCATALAASLRSSSDARRVAVAHIAFKVLGVALVFPFISPLTSLVAQTAGEVARQVAN
                     AHTLFNVAISALFLPFAPWAARVISALVPEEERGDNPYRTRYLDDRYLDQPALAVGQA
                     TREALRMGDVAQSMLRDAMVVLRTDNQELLEDVERRDDQLDYLDREIKLFIARLGRET
                     MSADMAQKEIALISFIGNLENIGDIIDKNLMELARKKLYQGRRFSEAGEAELIEFHSL
                     VSKNLERAIASFAANDRSLAQEVLDMRPLVRQRERELRDSHLARLRRGLAESLETSEI
                     HLDVLTNLKRISSHITALVYPILEEEP"
     gene            3218..4708
                     /gene="glgA"
                     /locus_tag="VFX87_04170"
     CDS             3218..4708
                     /gene="glgA"
                     /locus_tag="VFX87_04170"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX4994137.1"
                     /translation="MPSRLRILVVASEVAPFAKTGGLADVAGALPRALAALGHDVRVL
                     LPKYRGAEAHAGATRLVVPNIRVPLGDREAEGALIEGHAPSGVPVYLLEHEHYYNRDS
                     LYGTADGDYWDNCERFVFFCRAGLEGMARLDAGRPGIRWQPQVIHANDWQTGLLPVYL
                     RTLYRDHPMLGGLASVFTIHNLAYQGVFWHYDMPMTGLGWDLFTPAGIEFYGKVNFMK
                     GGLIFSDLLTTVSRTYAREIRTPAFGCGLEGVLEDRSQDLHGVVNGIDYEVWNPQKDP
                     AIAHPYSAEDPEPKAVCRETLRRDLSLEDGAGPVIGIIARLAEQKGMDLVLEALPGIL
                     AEGGQLVVLGSGDARLEEAFRAAAAAHPGQVAVRIGYDDDLSRRIYAGSDAFLMPSRY
                     EPCGLGQLIALRYGGAPIVRRTGGLADTVTEFDPARRSGTGFVFDAFTPDALLAAVRR
                     ASSTYRQPALWKALVRNAMAEDFSWDASAREYSTLYRKALRARSAR"
     gene            4723..5061
                     /locus_tag="VFX87_04175"
     CDS             4723..5061
                     /locus_tag="VFX87_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX4994138.1"
                     /translation="MKKIEAVIKPFKLDDVKEALNELGVVGMTVTEVRGFGRQKGHTE
                     LYRGSEYTIDFLPKVKIEVVVPDSLVEKVVSVICQSARTGSIGDGKVFVLSMGEAIRI
                     RTGERGEDAI"
     gene            5261..8320
                     /gene="glnE"
                     /locus_tag="VFX87_04180"
     CDS             5261..8320
                     /gene="glnE"
                     /locus_tag="VFX87_04180"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX4994139.1"
                     /translation="MNDLLAAAPDSRAALRQLKTLGFADPAAALRNLHALTPTPREAE
                     LLAPALPRLLAELAAAPDPDMALNNLERLASQGEHAAFLRLLSAHPGAIPLLARLGGT
                     SQFLADTLRRYPTLLPWLLEPRTMRQWLADELAEDLAVSIKAFVRPETRLNALRRFKY
                     RQLLRIGCRDILGDADLTVTTEELSHMADVCLAAAWDWARERLERLYGAPKAGDGAPT
                     GLAVIGMGKLGGEELNYSSDIDPVFVYGEDGLTSGGESGRIANGEFFAQAVRSIVDTL
                     ESVTEEGHVFRVDLRLRPEGRSGALILSVDGYRNYFADRAELWERQALIKARACAGDE
                     AVAARFFEAVRPFVYRTGLDPAIVRAVRGMKAQIDRALRGKDASHRNVKLGRGGIREV
                     EFLIQAMQLLYGGDDPWLRERNSLRAIFRLTERGYLSHALGRELGDALVYLRTVEHRL
                     QILHEFQTHTLPEDPRALGLLARRMGIAQPPATAARRFMVEHRRVTALVHRAFREFFA
                     AEPAAAPRPLRIPSHTALKATGFTDPDRARQNLRLVLEGRPLIPYPAAAGRAVARLFP
                     VLLDALWQSPDPDEALSQFERFVSAAGPRTAYLELLADRPELLINLVRLCTRGELVTE
                     LLIAQPELLNGLASPDTFAAHKRAPDFRRELAPVLAPRLAAAERPDVLRRLKQAQELG
                     IIWRMLLGVTDAERFSLEMTALAEATLAVAWMLALEETVAQHGVPRDAKGRLVPAAII
                     GLGKFGGRELTTGSDLDLFVVYGGAGFTDGEPPVEAHVFYDRAVEALSGLLSDITAAG
                     VAFAVDLRLRPGSKGSGFATSDDALERYYREWADPWERQTLTRARLVGGDPRLGRSVR
                     RRIRGLLYGPEAPPPDLKEMRALRERMERELGKETPGRFHVKFGRGGLVDVEFITQAL
                     QMRHGARYPGLRRANTLHAVREIAAAELLPADDCTTLAEQYRFLRRVSLGLRLFGTRP
                     GDTLELAGPNPARLAKTLDYPSRKEFIEDYRRRTAWVRALYDRVVPK"
BASE COUNT         1167 a         3079 c         2907 g         1247 t
ORIGIN      
        1 cccgcgcgag ggtgagacgg tggtggacga cgtgaaccgc ggccgtgtgg tgttcagcca
       61 cgccgagctc gacgactgga tcctcgtccg gaccgacggc acgccgacct acaacttctg
      121 cgtggtcgtg gacgacgtga ccatgaagat cacccacgtc atccgcggca gcgatcatct
      181 ctcgaacacg ccgaagcagg tgctctgcta ccgggccctc ggctacccgc tgccggtctt
      241 cgcccacatc ccgatgatcc tggccgcgga caagacgcgg ctctccaagc ggcacggcgc
      301 cacctccgtg ctggccttcc gcgacatggg tatcctgccc gaggccatgc tcaactacct
      361 cgcgcgtctc ggctggtccc acggagatca ggagatcttt tcccgagagg atctgatccg
      421 gcacttcgac ctggcccacg tgggcgcggc cgacgcggtc ttcgatctcg agaagttcac
      481 gtgggtcaac caccagtgga tcaagcggct gcccgccgcc cgcctcgcgg cagacctcgt
      541 gcccttcctc gcgcgggccg gcctgcccgc cccgcgtgac gtcgactggc tcgcgcgcgt
      601 ggcggacacg ctgaaggagc gggcgcgcac gctcgtggag atggccgagc aggccacgtt
      661 ctacctgcgg ccgcccgacg cctacgatcc gcagtctacc gcgaagttct ggaaggagca
      721 ggccgccgag cgctacgcca tcctgatcaa gcgcctcgag gcccaggagg cgatggatcc
      781 gcaggcgctg gaggagctct ttcgcttcct cgccgccgac ctcgggctca agctcgtgga
      841 cctggcgcag ctcacgcgca tcgcgctgac cggcaagacc gcgagcccgc ccatcttcga
      901 ggtggtgagc atcctgggca agccggagac gctcgcccgg ctcaaggccg ccgcggcggc
      961 ggtggaggcc cgccggtgag gcggctcctg ctcgcgcgcc acggccagtc cctctccaac
     1021 gcggtccggc gattccaggg ggcgcaggac gtggcgctgt cgccgctcgg cattcgccag
     1081 gcggaggcgc tgggcctggc ggtgggccgt cggtcgatcg cccacgtcta cgcgagcccg
     1141 ctcgagcgcg cccgccacac cgccctgatc gcgctggccg gcctcgacct cccgctgacc
     1201 ctggtggacg acctgcgcga gctgtcgctg ggcgagtggg aaggctgcac ggtcgaggag
     1261 atccgcacgc agcccggcga tccctatgcg cggtgggtgc gcgatccggt gcagtgcctg
     1321 ccgccgggcg gcgagcccct ggccgacgtg caggcgcgcg tgctccgtgc ggtggccgag
     1381 atcgtctcgg cgcaccccga tgaagaaaca attttgataa tttcgcacgg tggcgtgatc
     1441 agcgcgctgc tcgcgcactg gctcggcctg ccgctgtcct cgatctggcg gatcgccgtc
     1501 gccaactgct cgctctccga gatcgccccg ccgcgggtga tctcggtgaa cgagaccggc
     1561 cacctgcggg acatcgacgt cgtggtcgcc gcgccgctga gcccctgaca tgacgctgct
     1621 ggccctcttc ggcggcctcg ccttgctgct ctacggcatg cagctcatcg gcgagggcct
     1681 gcagcgcgcg gcgggcagtc acctgcgcca tctgctcacc agcatgaccc gcacccgcct
     1741 ggccgcggtg gcctcgggcg cgctggtgac ctcgatcatc cagtcctcgt cggccacgac
     1801 gctgatgctg atcggcttcg tctcggcggg gctcgtcacc ttcccccagt cgctcggggt
     1861 gatcctgggc gccgacatcg ggaccacggt gaccgtgcag ctcctcgcct tcaaggtcca
     1921 ggagctctcg ctgctcctgg tcggcgtcgg cttcgcgatg accttcttcg cgcgccgggg
     1981 cctggtgaag agcctggggc aggtgatcct gggcttcggc ttcatcttcc tcggcatgaa
     2041 ggtgatgaac gacgggctcg ccccgctcac cgagaacgag ctcacgcgcc aggtgctggt
     2101 ggccctcgcc gacaaccagt tcctcgcgct cctggtcggt gcggtgctga gcgcgagcat
     2161 ggcctcctcc gcggcgacta tcggcctgac cctttcgctc gggcaccagg gcctgctgcc
     2221 gctcggcgcc gcgatcccgg tggtgctggg cgcgaacatc ggcacctgcg ccaccgcgct
     2281 ggccgcgagc ctgcgctcct cctccgacgc gcgccgggtc gcggtggccc acatcgcgtt
     2341 caaggttctc ggggtggcgc tcgtcttccc cttcatctcg ccgctcacga gtctcgtcgc
     2401 ccagaccgcc ggggaggtgg cccgccaggt tgccaacgcc cacaccttgt tcaacgtggc
     2461 gatcagcgcg ctcttcctgc cgttcgcgcc gtgggcggcc cgcgtgatca gcgcgctggt
     2521 gccggaggag gagcggggcg acaatcccta ccgcacgcgc tacctcgacg accgttacct
     2581 ggaccagccg gccctcgccg tcgggcaggc gacgcgggag gcgctgcgca tgggcgacgt
     2641 cgcgcagagc atgctgcgcg acgcgatggt ggtgctccgc accgacaacc aggagctgct
     2701 ggaggacgtg gagcggcgtg acgaccagct cgactacctg gaccgcgaga tcaagctgtt
     2761 catcgcgcgg ctcgggcggg agaccatgag cgcggacatg gcccagaagg agatcgccct
     2821 gatctccttc atcggcaacc tcgagaacat cggcgacatc atcgacaaga acctgatgga
     2881 gctggcgcgc aagaagctct accagggccg ccgcttctcg gaggcgggcg aggcggagct
     2941 gatcgagttc cactcgctgg tctcgaagaa cctcgagcgg gccatcgcga gcttcgcggc
     3001 caacgaccgg agcctcgccc aggaggtgct ggacatgcgg ccgctggtgc ggcagcgcga
     3061 gcgcgagctg cgcgactcgc acctggcccg gctgcggcgc gggctggccg aatcgctcga
     3121 gacgtccgag atccacctcg acgtgctgac caacctcaag cgcatctcct cgcacatcac
     3181 cgcgctggtg tacccgatcc tggaagagga accctaggtg ccgagccggc tccgcatcct
     3241 cgtcgtcgcc tccgaggtcg cgcccttcgc caagaccggc gggctcgccg acgtggcggg
     3301 cgccctgccc cgcgccctcg ccgcgctcgg gcacgacgtg cgcgtcctgc tgcccaagta
     3361 ccggggcgcg gaggcccacg cgggcgcgac ccggctcgtg gtgcccaaca tccgggtgcc
     3421 gctcggggat cgggaggccg agggcgcgct gatcgagggg cacgccccgt ccggtgtccc
     3481 ggtgtacctg ctcgagcacg agcactacta caaccgcgac agcctctacg gcaccgccga
     3541 cggcgactac tgggacaact gcgagcgctt cgtcttcttc tgccgggccg ggctcgaggg
     3601 catggcgcgg ctcgacgcgg ggcgcccggg catccggtgg cagccgcagg tcatccacgc
     3661 caacgactgg cagaccgggc tgctgccggt ctacctgcgc acgctctatc gcgatcaccc
     3721 gatgctgggt ggcctggcca gcgtgttcac catccacaac ctggcctacc agggcgtgtt
     3781 ctggcactac gacatgccga tgaccgggct cgggtgggat ctctttacgc cggccgggat
     3841 cgagttctac ggcaaggtca acttcatgaa gggcgggctg atcttctccg atctcctgac
     3901 cacggtgagc cggacctacg cgcgcgagat ccgcacgccg gccttcggct gcgggctcga
     3961 gggcgtcctg gaggatcgta gccaggatct gcacggcgtg gtcaacggca tcgactacga
     4021 ggtgtggaac ccccagaagg atcccgccat cgcccacccc tactcggcgg aggacccgga
     4081 gccgaaggcg gtctgccgcg agaccctgcg gcgcgacctc agcctcgagg acggcgcggg
     4141 gccggtcatc gggatcatcg cccggctcgc cgagcagaag ggcatggatc tcgtgctcga
     4201 ggccctgccc ggcatcctgg cggagggcgg ccagctcgtg gtgttgggct cgggcgacgc
     4261 gcggctcgag gaggccttcc gcgccgccgc ggccgcgcac ccgggccagg tggcggtgcg
     4321 catcggctac gacgacgacc tctcccgccg catctacgcg ggcagcgacg cgttcctgat
     4381 gccctcgcgc tacgagccgt gcgggctggg gcagctcatc gcgctccgct acgggggggc
     4441 gccgatcgtg cggcgcaccg gcgggctcgc cgacaccgtc accgagttcg acccggcccg
     4501 gcgaagcggc accgggttcg tgttcgacgc gttcacgccg gacgccctgc tcgccgcggt
     4561 ccgccgagcc tcctcgacgt accggcagcc cgcgctgtgg aaggccctgg tgcgtaatgc
     4621 gatggccgag gacttctcct gggacgcctc ggcgcgggaa tactctactc tctaccgcaa
     4681 ggcgctgagg gcgcgtagcg cccgctagcc acggaggccg gcatgaagaa gatcgaggcg
     4741 gtgatcaagc cgttcaagct cgacgacgtc aaggaggcgc tcaacgagct cggggtcgtc
     4801 ggcatgaccg tcaccgaggt gcgcggcttc ggacgccaga agggccacac cgagctctat
     4861 cgcggctcgg aatacacgat cgacttcctg cccaaggtga agatcgaggt cgtggtgccc
     4921 gacagcctgg tggagaaggt ggtgtcggtg atctgccagt ccgccaggac cggctcgatc
     4981 ggcgacggca aggtcttcgt cctctcgatg ggcgaagcca tccgcatccg caccggggag
     5041 agaggcgagg acgccatctg agcttctctc cccctctccc ctccggggag aggccagggt
     5101 gaggggcgtt tgggtcctgt ggagtcaacc gttccggcgt ggtcgatcat ttacttcttc
     5161 aggctctcca tatgcggggg cccagaaatg gcccccgcac tcccccagat agcggacaat
     5221 ccgtgatggc ccccgcactc ccccagctag cggacaaccc gtgaacgact tgctcgcggc
     5281 cgcgccagat agtcgggccg cgctgcgcca gctaaagacg ctgggcttcg ccgaccccgc
     5341 ggcggcgctg cgtaacctgc acgcgctgac gcccacgcca cgcgaggccg agctgctcgc
     5401 gcccgcgctg ccccgcctgc tggccgagct ggccgcggcg cccgatccgg acatggcgct
     5461 caacaatctc gagcgcctgg cttcgcaggg cgagcacgcc gcgttcctcc gcctgctgag
     5521 cgcgcatccg ggagccatcc cgctgctggc ccgcctgggc ggcaccagcc agttcctggc
     5581 cgacaccctc cgccgctacc ccacgctgct gccctggctg ctcgagccgc ggacgatgcg
     5641 gcagtggctt gccgatgagc tcgcggagga cctcgccgtg agcatcaagg ccttcgtgcg
     5701 gccggagacg cggctcaacg cgctccggcg cttcaagtac cgccagctcc tgcgcatcgg
     5761 ctgccgcgac atcctgggcg acgccgacct caccgtgacc accgaggagc tgtcccacat
     5821 ggccgacgtg tgcctggccg cggcctggga ctgggcccgg gagcggctcg agcggctcta
     5881 cggggcgccg aaggcaggcg acggcgcgcc gaccgggctc gcggtcatcg gcatgggcaa
     5941 gctcgggggc gaggagctca actactcctc ggacatcgac ccggttttcg tctacgggga
     6001 ggacggcctg acctcgggcg gcgaatcggg ccgcatcgcc aacggcgagt tcttcgccca
     6061 ggcggtccgg tccatcgtgg acacgctcga gtcggtgacc gaggagggcc acgtcttccg
     6121 cgtggacctg cgcctgcgcc ccgaggggcg ctcgggcgcg ctgatcctgt cggtggacgg
     6181 ctatcgcaac tacttcgccg atcgcgccga gctctgggag cgccaggccc tcatcaaggc
     6241 gcgcgcctgc gcgggcgatg aggcggtggc cgcgcgcttc ttcgaggcgg tgcgcccctt
     6301 cgtctaccgg accgggctcg acccggccat cgtgcgcgcg gtgcgcggca tgaaggccca
     6361 gatcgaccgc gcgctccgag gcaaggacgc gtcgcaccgc aacgtgaagc tcggccgcgg
     6421 cggtatccgc gaggtggagt tcctcatcca ggccatgcag ctcctctacg gcggcgacga
     6481 cccgtggctg cgcgagcgga acagcctgcg cgcgatcttc cgcctcaccg agcggggcta
     6541 cctgtcgcat gcgctcgggc gcgagctggg cgacgcgctc gtgtacctgc gcaccgtcga
     6601 gcaccggctg cagatcctcc acgagttcca gacccacacg ctcccggagg acccgcgtgc
     6661 gctcggactc ctggcccgcc gcatgggcat cgcgcagccc cccgcgacgg cggcgcggcg
     6721 cttcatggtc gagcaccggc gcgtgaccgc gctggtgcac cgcgccttcc gcgagttctt
     6781 cgccgccgag ccggccgcgg ccccgcgccc gctgcgcatc ccgagccaca ccgcgctcaa
     6841 ggcgaccggc ttcaccgacc cggaccgcgc ccgccagaat ctccggctgg tcctggaagg
     6901 ccgcccgctg atcccgtacc cggcggcggc cggccgcgcg gtggcccgcc tcttcccggt
     6961 gctgctggac gcgctctggc agagcccgga tcccgacgag gcgctgagcc agttcgagcg
     7021 cttcgtctcg gccgcggggc ccagaaccgc ctacctcgag ctcctggccg accggccgga
     7081 gctcctcatc aacctggtcc ggctctgcac gcggggcgag ctggtgaccg agctcctgat
     7141 cgcccagccg gagctgctca acggcctcgc gagcccggac accttcgcgg cgcacaagcg
     7201 ggcgcccgac ttccgccggg agctggcgcc cgtgctcgcg ccgcgcctgg cggccgcgga
     7261 gcgcccggac gtcctgcggc ggctcaagca ggcgcaggag ctcggcatca tctggcgcat
     7321 gctgctcggg gtgacggacg cggagcgctt ctcgctcgag atgaccgcgc tcgccgaggc
     7381 cacgctcgcg gtggcgtgga tgctggccct cgaggagacg gtggcccagc acggcgtccc
     7441 gcgcgacgcg aagggccggc tcgtgcccgc ggctatcatc ggcctcggca agttcggcgg
     7501 ccgtgagctc acgaccggct cggacctcga tctcttcgtg gtgtacgggg gcgccggctt
     7561 caccgacggg gagcccccgg tcgaggcgca cgtgttctac gatcgcgcgg tcgaggcgct
     7621 gagcggtctc ctgagcgaca tcacggccgc cggcgtggcg ttcgcggtgg acctgcgcct
     7681 gcgccccggc tccaagggca gcggcttcgc cacgagcgac gatgcgctcg agcgctacta
     7741 ccgcgagtgg gcggatccgt gggagcgcca gacgctcacg cgcgcccggc tcgtgggcgg
     7801 cgatccgcgc ctcggccggt cggtgcgccg gcgtatccgc gggctgctct acgggcccga
     7861 ggcgccgccg cccgatctca aggagatgcg cgccctgcgc gagcgcatgg agcgcgaact
     7921 cggcaaggag acgccgggcc ggttccacgt gaagttcggg cgcggcgggc tcgtggacgt
     7981 ggagttcatc acccaggctc tgcagatgcg ccacggggcc cggtatccgg gccttcgccg
     8041 cgccaatacc ctccacgccg tccgcgagat cgcggcggcc gaactgctcc ccgcggacga
     8101 ttgcaccacc ctcgccgagc agtaccgctt cctgcgccgg gtgtcgctcg ggctccggct
     8161 cttcggcacg cgccccggcg atacgctcga gctggcgggc ccgaacccgg cccggctcgc
     8221 caagaccctg gactatccgt cgcggaagga gttcatcgag gactatcgtc gccgcaccgc
     8281 gtgggtgcgg gcgctctacg accgcgtcgt gccgaagtga cccggcgcgt gaatccgcta
     8341 cgggctcgcg gagcgaccgc aggaaagcga gcacctcacg cagaagacgc ggaccacgcc
//
LOCUS       DASUFA010000113         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_714257, whole genome shotgun sequence.
ACCESSION   DASUFA010000113 DASUFA010000000
VERSION     DASUFA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714257"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFX87_04185"
     CDS             <1..767
                     /locus_tag="VFX87_04185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994140.1"
                     /translation="TSPNIGAGIAGGLAEEGAKVVCVDVSADNARECAAWITGRGGQA
                     MGLTCDVSDEAQVEAMVARAREAYGGIDVLVNNAGILGGLSVLEMPLDRWSRQLAVNL
                     TGTFLCTKHVARLMVERGTRGSIIVIVSTAGHQGQPGNIGYCTSKSGLLNFTRAASMD
                     LARHGIRVNSLTPTATDAEEGDERAEAWGRPRPERRAGLLDFATMIPTGTLPSPRHYA
                     RAAVFLASDDAEMITGIDLRVDAGAVAKYWPWVPSA"
     gene            982..1182
                     /locus_tag="VFX87_04190"
     CDS             982..1182
                     /locus_tag="VFX87_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX4994141.1"
                     /translation="MAQGTVKWFNDAKGYGFITQEAGDDVFVHFNAIQAQGFKSLAEG
                     DKVEFEVTKGPKGLQAANVRKI"
     gene            1269..2360
                     /locus_tag="VFX87_04195"
     CDS             1269..2360
                     /locus_tag="VFX87_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4994142.1"
                     /translation="MVSALEHVAWEPCVLELHRDRTLESYARKRQGIPNPAIGYFAPV
                     PWLARALIDLHPEYGLLMRLDHDVMDLVALAVSQENSCRFCYAAVRAMLWAQGMSRAR
                     IQRIEQDLARADLSPRAVAAMAFGRSQSRSGPVGARAARDALRQAGIDADEMREIAYA
                     VALTDFSNRAYTIPAIPARPIERMPDQLHMRLLRPLLNRLLRRSRYPGRPTALEPVPP
                     SPYSRLVQAYAGSPIATAVARTLEDMWASPHLTRRCKLLMLAVVARGLACEACAPEIS
                     EALQREGVTEATLARVLTHLDAPELDPAERLLVPFARETIWYEPAPMQRRTRLLRERL
                     SGPQLVEAIGVASLANGLCRMGAMVLDPA"
     gene            2357..>2603
                     /locus_tag="VFX87_04200"
     CDS             2357..>2603
                     /locus_tag="VFX87_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994143.1"
                     /translation="MTRAVLVGAALLLLAVLAAALYRQRRRADSLQAQLDAAAAELQH
                     LQEACSRLAPAGVVQRLVADGVRASPAVVAERKVVTAL"
BASE COUNT          368 a          918 c          931 g          386 t
ORIGIN      
        1 ggacgagccc caatatcggc gccggcatcg ccggcgggct ggcggaggag ggggcgaagg
       61 tcgtctgcgt ggacgtgagc gcggacaatg cgcgcgagtg cgcggcctgg atcacgggcc
      121 ggggcggcca ggccatgggc ctcacctgcg acgtgtcgga cgaggcccag gtggaggcca
      181 tggtcgcgcg ggcgcgcgag gcctacggcg ggatcgacgt gctggtcaac aatgcgggca
      241 tcctgggcgg cctgagcgtg ctggagatgc cgctcgaccg ctggagccgg cagctcgcgg
      301 tgaacctcac cgggaccttt ctctgcacca agcacgtggc ccggctcatg gtcgagcgcg
      361 gcacccgggg cagcatcatc gtgatcgtct ccaccgccgg gcaccagggc cagccgggga
      421 atatcggcta ctgcacgagc aagagcggcc tgctcaactt cacgcgcgcc gcctccatgg
      481 acctcgcgcg gcacgggatc cgcgtgaaca gcctcacgcc gaccgcgacc gacgcggagg
      541 agggtgacga gcgcgcggag gcgtgggggc gcccgcggcc cgagcgccgc gccgggctgc
      601 tcgacttcgc gacgatgatc ccgacgggga cgttgcccag cccgcggcac tacgcgcgcg
      661 ctgcggtctt cctcgcctcc gacgacgcgg agatgatcac cggcatcgac cttcgcgtgg
      721 acgcaggcgc ggtggcgaaa tactggccct gggtgcccag cgcgtgacct cgtgatattg
      781 cttgacagaa cgcgagccgg gctgtactgt gtgctcaggt ttcaggttca tccggttgca
      841 gatttctcga gattcgagat caaacgccgt gaggacccct ggtcccgcgg cgtttttgct
      901 tgtcctccat ttcaggaggg ccccccgcca tggtggcggg tttcaccgag gcgcgagcct
      961 cagaataaag aggtagtttc aatggcacag ggaacggtca agtggttcaa cgatgcgaag
     1021 ggctatggct tcatcaccca ggaggccggc gacgacgtgt tcgtgcactt caacgccatc
     1081 caggcgcagg gcttcaagag cctcgccgag ggcgataagg tcgagttcga ggtgaccaag
     1141 ggccccaagg ggctccaggc cgccaacgtc cgcaagatct gacgccaacg tcacgttgag
     1201 ccggaggccg ggtccccatc gggggcccgg ccttctgtgc tttcacggcc tgcgctagag
     1261 taggccccat ggtcagtgcc ctcgagcatg tcgcctggga gccgtgtgtg ctcgagctcc
     1321 accgggaccg caccctggag tcctatgccc ggaagcggca gggcatcccc aacccggcca
     1381 tcggctactt cgcgccggtc ccctggctgg cccgcgcgct gatcgacctg catcccgagt
     1441 acgggctcct gatgcgcctc gaccacgacg tgatggacct cgtggcgctg gcggtgagcc
     1501 aggagaactc gtgccgcttc tgctacgccg cggtgcgggc gatgctgtgg gcccagggca
     1561 tgagccgggc gcgcatacag cgcatcgagc aggacctggc gcgggccgat ctctccccgc
     1621 gggcggtcgc cgccatggcc ttcgggcgga gccagtcgcg ctccggtccg gtcggggcgc
     1681 gcgcggcccg ggacgccctc cggcaggcgg gcatcgacgc cgacgagatg agggagatcg
     1741 cgtacgcggt ggccctcacg gatttcagca accgcgccta taccatcccg gccatccccg
     1801 cgcggccgat cgagcgcatg cccgatcagc tccacatgcg cctgctccgc ccgctgctca
     1861 accggctcct gcgacgcagc cgctaccccg ggcggcccac ggcgctggaa ccggtgccgc
     1921 cgtcacccta ttcccgcctc gtgcaggcgt acgcgggatc cccgatcgcg acggcggtgg
     1981 cccggacgct cgaggacatg tgggcctcgc cccatctcac gcgccggtgc aagctcctga
     2041 tgctggccgt cgtggcgcgg ggcctggcgt gcgaggcgtg cgcgcccgag atcagcgagg
     2101 cccttcagcg ggaaggcgtg acggaggcga cgctcgcccg ggtcctgacc catctcgacg
     2161 cccccgagct cgatcccgcg gagcggctgc tcgtgccctt cgcccgtgag acgatctggt
     2221 acgagccggc cccgatgcag cgccggacgc gacttctgcg cgagcggctt tccggcccgc
     2281 agctcgtcga ggcgatcggg gtggcctcgc tcgccaacgg gctctgccgg atgggcgcca
     2341 tggtgctgga tcccgcatga cgcgcgccgt cctcgtcggc gcggcgctgc tcctgctggc
     2401 cgtcctcgcc gccgcgctct atcgccagcg gcggcgggcc gacagcctcc aggcgcagct
     2461 cgacgcggcc gccgcggagc tccagcacct ccaggaggcc tgctcccggc tggcgccggc
     2521 cggcgtcgtg cagcggctcg ttgccgatgg cgtgagggcg agcccggcgg tcgtcgccga
     2581 gcgcaaggtc gtgaccgcgc tgt
//
LOCUS       DASUFA010000114         4681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_714414, whole genome shotgun sequence.
ACCESSION   DASUFA010000114 DASUFA010000000
VERSION     DASUFA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714414"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFX87_04205"
     CDS             <1..367
                     /locus_tag="VFX87_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4994144.1"
                     /translation="DEPMPTIVADANAIGQVLLNLLTNARRAMPEGGEITIETSHLAA
                     ARSVRLSVRDTGTGISPEIMPKIFDPFFTTKPEGTGLGLSISHGIMHDHHGTLDVRSE
                     VGKGSTFTLTFPLDAAPAG"
     gene            complement(376..1818)
                     /locus_tag="VFX87_04210"
     CDS             complement(376..1818)
                     /locus_tag="VFX87_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX4994145.1"
                     /translation="MSKSATNRPQSAFEVFEEAFARAAGTPLVNGNEVRLLRDAGENY
                     PAWLEAIAAARRTIHFESYIIHDDETGQEFARALRARAAAGVRVRVIYDWLGAVGKTS
                     RRFWRDLREGGIEVRCFNPPGLSSPFAWISRDHRKMLAVDGRVAFVTGLCVGRAWVGD
                     PARGVEPWRDTGIAVRGPAVADLESAFARMWALAGAPISDRELPAREALPPVGDVAVR
                     VIAGMPGTAELYRLDQLIAAAARETLWLTDAYFVGTTAYVQSLRAAARDGVDVRLLVP
                     GASDIWVLRGLSRAGYRPLLEGGVRVYEWNGPMLHAKTAVADGRWARVGSTNLNLQSW
                     IGNWELDVAVEDEDFAGRMEDMYLDDLGRATEITLTRRARVVRTAGAPRLPVRRPGSA
                     GRAAAGAVSIGSAVGAAMTGRRVLGPAEARIMAAAGVALLALGLVALLWPWAVAVPLA
                     ALAIWLAASLLFRAWALRRERTEEPRDTEG"
     gene            1861..2757
                     /locus_tag="VFX87_04215"
     CDS             1861..2757
                     /locus_tag="VFX87_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX4994146.1"
                     /translation="MNAPEPGRRGEIPAARLDALLPPEMAERAEEIGVRKAGLDLPRL
                     FTLAVLAGAFIALGGVFATTALAGAGAAPWGPVRVLAGAAFSLGLILVVVGGAELFTG
                     NNLVVMAWASGRVSSAALLRNWVVVFVGNFAGATATALAVCLAGTQRGGEGAFGVAAF
                     AIAHAKLQPRFFQAVVLGVLCNALVCLAVWLTYSARTTADRILAIVPPISAFVAAGFE
                     HSIANMYFVPYAMFVAAFDPDFVTGRELGSQAQALTWATFLGRNLLPVTIGNIIGGSV
                     LVGAVYWFAYLRQRGPMSRSRE"
     gene            complement(2747..3739)
                     /locus_tag="VFX87_04220"
     CDS             complement(2747..3739)
                     /locus_tag="VFX87_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX4994147.1"
                     /translation="MKAAVVTARSGAITRPLEIRDVPDPGLTPGHCLLRVLACGVCRT
                     DLHVVEGDLRPVRDRLIPGHQIVGEVVGGATDELPRGTRVGVSWLGGTDGHCRYCRSG
                     QENLCDSPTFTGYTVDGGYAEYAVARSDFVFPLPAALGDLQAAPLLCAGVIGFRSLRV
                     AGVEPGDRVGLFGFGASAHLALPVLRSWSCEVFVSTRGASHRRLAVSLGATWVGDDTH
                     RPPVTLDRAITFAPSGDVVVAALACLRKGGVVAINAIHLDRIPEFDYDRLLWGERQIR
                     SVTNMTRDDAREFLQVASAIGLRPSVTAFPLDRANEALRALKDDAIDGAAVIIP"
     gene            complement(3736..>4681)
                     /locus_tag="VFX87_04225"
     CDS             complement(3736..>4681)
                     /locus_tag="VFX87_04225"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX4994148.1"
                     /translation="LGFDDVSGIVAQGGTMLGTSNRDDPFRVPVDRPGERAFEDRSDV
                     VLRTLARHGIEGLLVIGGDGSLTIGRRLEPKGVAVVGIPKTIDNDVSGTDVTLGFDSA
                     LTVATEAVDRLHTTAASHHRVMVVEVMGRNAGWIALEAGLAGGGDVILIPEIPFRYEG
                     VAAGILDRARRGRRFSIVVVAEGVTCPGRGPVVRQIVGDSPDPVRLGGVGAVLAQALE
                     GLVPFEVRYVVLGHVQRGGSPTPFDRMLATRFGAAAVAALADGASATMVALRGGRIER
                     VSLADALSRPKRVDPQGEHVAAARGIGTIFGDETRPEA"
BASE COUNT          680 a         1794 c         1589 g          618 t
ORIGIN      
        1 ggacgagccc atgcccacca tcgtggccga cgcgaacgcg atcgggcagg tgctcctcaa
       61 cctgctcacc aacgcccggc gggccatgcc cgagggcggc gagatcacga tcgagaccag
      121 ccacctggcc gccgcccggt cggtccgcct ctcggtgcgg gataccggca ccgggatttc
      181 ccccgagatc atgccgaaga tcttcgaccc gttcttcacc acgaagccgg aagggaccgg
      241 gctcggcctc tcgatcagcc acggcatcat gcacgatcac cacgggacac tggacgtccg
      301 ctccgaggtc ggcaagggga gcacgttcac gctgaccttc ccgctcgacg ccgcgcccgc
      361 cggctgatct cgcggtcaac cctcggtgtc cctcggctcc tcggtccgct cgcgacgcag
      421 ggcccaggcg cgaaacagca ggctcgcggc cagccagatc gcgagcgcgg ccagcggcac
      481 cgcgactgcc cagggccaga gcagtgccac cagacccagc gccaggagcg ccacgcccgc
      541 cgcggccatg atccgggcct cggccgggcc cagcacgcgg cggcccgtca tggcggcccc
      601 caccgcgctg ccgatgctca ctgcccccgc cgcggcccgc cccgcgctcc ccggacggcg
      661 gacgggaagg cgcggcgccc ccgcggtgcg cacgacgcgg gcccggcgcg tgagcgtgat
      721 ctcggtcgcg cggccgaggt cgtcgaggta catgtcctcc atccggcccg cgaagtcctc
      781 gtcctccacc gccacgtcca gctcccagtt gccgatccac gactggagat tgagattcgt
      841 cgagcccacg cgcgcccatc gcccgtccgc gaccgcggtc ttggcgtgga gcatcgggcc
      901 gttccactcg tagacgcgca cgccgccctc gaggagcggg cggtaccccg ccctcgagag
      961 gccgcgcagg acccagatgt cgctcgcgcc gggcaccagc aggcgcacgt ccacgccgtc
     1021 gcgggccgct gcgcggagcg actgcacgta cgcggtcgtg ccgacgaagt acgcgtcggt
     1081 gagccagagg gtttcccgcg cggcggccgc gatgagctgg tccaggcgat agagctcggc
     1141 ggtcccgggc atccccgcga tgacgcgcac cgccacgtcc cccaccgggg gcagcgcctc
     1201 ccgcgcgggc agctcccggt ccgagatcgg agcgccggcc agcgcccaca tccgggcgaa
     1261 tgccgattcc agatcggcga ccgccggccc gcggaccgcg atgccggtgt cgcgccaggg
     1321 ttcgacaccc cgggccggat cgccgaccca ggcacggccc acgcagagcc cggtgacgaa
     1381 ggcgacgcgc ccgtccaccg ccagcatctt ccggtggtcg cgggagatcc acgcgaacgg
     1441 gctcgacagg ccagggggat tgaagcagcg gacctcgatg ccgccctcgc gcaggtcacg
     1501 ccagaatcgc ctggaggtct tgcccaccgc gccgagccag tcgtagatca cgcgcacccg
     1561 cacgcccgcg gcggccctgg ccctgagcgc ccgggcgaac tcctgcccgg tctcgtcgtc
     1621 gtgaatgatg tagctctcga agtggatcgt gcggcgggcc gccgcgatgg cctcgagcca
     1681 ggccggatag ttctcccccg cgtcccggag cagccgcacc tcgttgccgt tgacgagtgg
     1741 cgtccccgcg gcgcgggcga acgcctcctc gaacacctcg aacgcggact gcggccgatt
     1801 cgtcgccgac ttcgacatgg accgattatg ctacacaccg acaggatgcc tggccagccc
     1861 atgaacgcac cggagcccgg gcgccgcggc gagatccccg cggcgcgact ggacgccctg
     1921 ctcccgcccg agatggcgga gcgcgcggag gagatcgggg tccgcaaggc cggccttgat
     1981 ctgccccgcc tcttcacgct cgcggtcctc gccggggcgt tcatcgcgct cggcggcgtg
     2041 ttcgccacca ccgccctggc cggggcgggc gcggccccct gggggccggt ccgggtgctc
     2101 gcgggcgcgg ccttcagcct cgggctcatc ctcgtcgtgg tgggcggggc ggagctcttc
     2161 accgggaaca acctcgtcgt gatggcatgg gccagcggcc gggtgagcag cgcggccctg
     2221 ctgcgcaact gggtggtcgt cttcgtgggc aacttcgcgg gcgcgaccgc caccgcgctc
     2281 gcggtgtgcc tggcggggac tcagcgcggg ggcgagggcg ccttcggcgt cgccgccttc
     2341 gcgatcgccc acgccaagct ccagccgcgc ttcttccagg ccgtcgtcct cggagtcctc
     2401 tgcaacgccc tggtgtgcct cgcggtctgg ctcacctaca gcgcgcgaac gaccgccgac
     2461 cggatcctcg cgatcgtgcc cccgatcagc gccttcgtgg ccgccggctt cgagcactcg
     2521 atcgcgaaca tgtacttcgt gccctacgcg atgttcgtcg ccgccttcga cccggacttc
     2581 gtgaccggcc gagagctcgg atcccaggcg caggcgctca cgtgggctac ctttctcggc
     2641 cgcaacctgt tgccggtcac gatcgggaac atcatcggcg gctcggtcct ggtgggcgct
     2701 gtgtactggt tcgcgtacct ccgtcaacgc ggccccatga gccgctctag ggaatgatga
     2761 cggccgctcc atcgatcgcg tcgtccttga gggcgcggag ggcttcgttc gcgcgatcga
     2821 gcggaaaggc ggtcaccgag ggccgaagcc cgatcgcgct cgcgacctga aggaactctc
     2881 gcgcatcgtc tcgagtcatg ttcgtcacgc tccgaatctg ccgctcgccc cacaggagac
     2941 ggtcgtagtc gaactcgggg atccgatcca gatgaatggc gttgatcgcc acgacgcccc
     3001 ccttccgcag gcacgcgagc gccgcgacca cgacgtcgcc gctgggagcg aacgtgatcg
     3061 cacgatcgag cgtgacgggc gggcggtgcg tgtcatcgcc gacccaggtg gccccgagcg
     3121 agaccgccag ccggcggtgc gacgcacccc gggtggagac gaacacctcg cagctccagg
     3181 atcgcagcac gggaagtgcc agatgggcgg acgccccgaa cccgaacagg ccgacccggt
     3241 cgcccggttc gacgccggcc acccgcaggc tgcggaagcc gatgactccg gcgcatagca
     3301 gcggagccgc ctggagatcg cccagggcgg cggggagagg aaacacgaaa tcgctgcggg
     3361 cgaccgcgta ttccgcatag ccgccgtcca ccgtgtaccc ggtgaaggtg ggcgagtcgc
     3421 acaggttctc ctggccgctc cggcaatagc ggcagtgccc gtccgtcccc cccagccacg
     3481 agaccccgac gcgcgtgccg cggggcagct cgtccgtcgc ccccccgacc acctcgccca
     3541 cgatctgatg acccggaatc aggcggtcac ggaccggccg caggtctcct tcgacgacgt
     3601 gcaggtccgt ccggcacacg ccacaggcga gcactcgcaa gagacagtgc cccggcgtga
     3661 ggcccgggtc cgggacgtcg cggatctcca gaggccgcgt gatggcaccg gaccgggcgg
     3721 tgacaacggc ggctttcacg cctccggccg ggtctcgtcg ccgaagatcg tcccgatgcc
     3781 gcgcgcggcg gccacgtgct cgccctgcgg gtccacgcgc tttggtctgg agagggcatc
     3841 cgccagggag acgcgctcga tccggccgcc ccggagcgcg accatggtgg ccgacgcccc
     3901 gtccgccagc gccgccaccg ccgcggcgcc gaagcgggtc gccagcatcc ggtcgaacgg
     3961 ggtgggcgag ccccctcgct ggacgtgccc gagcaccacg tagcgcacct cgaacggtac
     4021 gaggccctcg agggcttgag ccagcaccgc ccccaccccg ccgagccgca cgggatccgg
     4081 gctgtccccg acgatctggc gaaccacggg gcctcggccc gggcatgtga ccccttcggc
     4141 caccacgacg atcgagaagc ggcgcccgcg ccgcgcgcgg tcgaggatcc cggccgcgac
     4201 cccctcgtag cgaaacggta tctcgggaat caggatcacg tccccgccac ccgccagtcc
     4261 cgcctcgagc gcgatccatc cggcatttcg ccccatcact tcgaccacca tgacccggtg
     4321 gtgggacgcc gccgtggtgt gcagccggtc cacggcctcg gtggccaccg tcagggcgga
     4381 gtcgaagccg agggtcacgt cggtgccgct gacgtcgttg tcgatggtct tgggaatccc
     4441 gacgaccgcg acacccttgg gctcgagcct ccgcccgatc gtcaagctcc cgtccccacc
     4501 gatgaccagc agcccctcga tgccgtgccg cgccagtgtc ctcagcacca cgtcggagcg
     4561 gtcctcgaag gcgcgctcgc ccggccggtc caccgggacg cgaaacggat cgtcgcggtt
     4621 cgacgtgccg agcatggtcc cgccctgagc gacgatgccg gagacgtcgt cgaagccgag
     4681 c
//
LOCUS       DASUFA010000115         7120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_357440, whole genome shotgun sequence.
ACCESSION   DASUFA010000115 DASUFA010000000
VERSION     DASUFA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357440"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFX87_04230"
     CDS             complement(<1..970)
                     /locus_tag="VFX87_04230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4994149.1"
                     /translation="MRVGLLPALGGGIRALAQSGQQSRLIDGYFRPYLDAFDGLDYFS
                     YLPESLAEFTADPMLRERVRVLAPPGPMARGRRAVQIPVAHAAEMRQCAALRVFQITG
                     VIPALIARRRFGIPYVTTYGFWYAQLSQPGPKRALKALVERLGLKHAAAVIATTPDLA
                     ARASRLARRVELIPNGVDTTLFRPAVSRPAGTPTHILYVGRLSAEKNLSTVVTAAGSI
                     GAGSGGHVTLTLVGSGALRDDLEAQAKRLGVVVDFPGVVDQRQLPDVYGAANIFVLAS
                     FTEGHPKVLLEAMACGVPCVASNVGGSRSILADGESGLLFDPRDAGA"
     gene            complement(970..1758)
                     /locus_tag="VFX87_04235"
     CDS             complement(970..1758)
                     /locus_tag="VFX87_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4994150.1"
                     /translation="MNGLQRLLVRGVDVVNRRGKSVGVRLVKLTGKRPYAIHPKHLVE
                     QPWHDWYLPYLDRRDFVLDVGCSNGAHLVRAATACRSIVGFDYDLPQLAIAGRTIKEQ
                     RLERARVLAWDITGAFPFPDRTFDKALFLDVIEHLVPRVAVLREIRRVLKDDGRLLVS
                     GPNRENSWRRTLRAAGLFSYSDPDHKVEYTREEFLAELQEAGFEPEGAVMPVVLDTPW
                     AGVIDAVGGVSLSLYDRLSRWKREAALRRPEESTGFRVVARKRP"
     gene            complement(1755..2906)
                     /locus_tag="VFX87_04240"
     CDS             complement(1755..2906)
                     /locus_tag="VFX87_04240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4994151.1"
                     /translation="MRICTPHCGIAPETTSGGETYERELLERLGRAGVRLEIILARGK
                     PHRPDVPNWTVHRFGIGRGLRWYVAPFVVPAAIRRVYEMVGFDLLRVHSLRYIGPSAL
                     AARRWYGLDVPVVSHHHHLDPSPLNRLIEKRVVNASDRVVVGSEFARRQLREELGART
                     DHVSVVYYGVDPRFTPRPPSEALRARYGLTGRRIVLFFGGLKPRKNLGLLLEAWRVLA
                     PRHPDTTLLVAGGGSLLPELRAHAGRLGLDGRVVFTGYVAEAEKPEHFNLGDVFFFPS
                     AMEGFGLTVAEAMSSGLPVVVSNRGSIPELVVDGEGGFVCDPAGPGPFVEKLALILGD
                     RALGEKLGRANRERVERMFRWERCVDGTRRVYETVLEEWRHRGGGKGAR"
     gene            complement(2914..3579)
                     /locus_tag="VFX87_04245"
     CDS             complement(2914..3579)
                     /locus_tag="VFX87_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4994152.1"
                     /translation="MSRGLAYYLGVSYRRKRVDADLTAVSGHFSGRVLDVGGARRRGA
                     FRPPPAARWVVADIERTHRPHVGADVQALPFRDGAFDAIKATELLEHVPDVARALAEC
                     RRVLRPGGHLVITVPFLERLHGDPGDYARYTRSMWERLLAEADLKPVTIAEQGGYFTH
                     LAGMLRFLVQRAPRPLRWVGYGFFPLLDLLTRLDSLRRARESDFAAFVGGYLIVAERP
                     PAS"
     gene            complement(3576..4859)
                     /locus_tag="VFX87_04250"
     CDS             complement(3576..4859)
                     /locus_tag="VFX87_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4994153.1"
                     /translation="MTSVLFASEYYPPFAPGGAEWSTAAWAAALTRRGHRVTVVTPNY
                     GAAAREERDGATIIRVPFPVKLRPGQGEVRFLVHRNPLFHLYFAWQVRRAARAAGAQI
                     IHAQGKAALVGCWLAGRSLGRPVLVTIRDLGLLCPLGHCTVFEPWERFDCAATQYLGK
                     CLPYHLTHYAAGLGGLGRARVRVAAVAAWIDNRLRQQAMRRADGLLGVSQGILDVYPI
                     RLLGPRARVVPNLPPPVAVPSDAEARAARDRLGVGAAPLVLYAGKLSIGKGTPVLLDA
                     LDRIAAAVPGARFALAGKGDMAIPARPDLRRLGVLPQAELFALYRAADVVVVPSVWPE
                     PLSRVLLEVMRIGRPVVATAVGGTPEAVEHGVTGLLVPRQDPEALAKAVIELLLDPER
                     RARMGRAAAERARTAFDEARLVTALLEVYRKAAVA"
     gene            complement(4856..5629)
                     /locus_tag="VFX87_04255"
     CDS             complement(4856..5629)
                     /locus_tag="VFX87_04255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994154.1"
                     /translation="MSRAVELRDRLRRYYTTYYRDVLGIPDWSVLVGLREEEERQERG
                     RLDRLRRLLGGEVRGRILNVGCGTGGFNLVAAEAGARAVGVDADAEAIGICALKREKG
                     GGTFVRAAAERLPFPGDTFDVVYCFSAIEHVESVEESVAEMVRVTRRGGLIYVHTPNA
                     WSWYEGHYKLLWAPFLPAALGRLYLRARGRPSGYLATLRRLTPRAMRRAFARAGVVDL
                     RFHDDAAPRESLGPLRVPIGLYYRLSGVSPFIEVVARKP"
     gene            complement(5626..6789)
                     /locus_tag="VFX87_04260"
     CDS             complement(5626..6789)
                     /locus_tag="VFX87_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HEX4994155.1"
                     /translation="MTAPRRVIPNAVPHLAGNEWKYVKECLDTNWVSSAGPFVDRFER
                     EVAQYLGVPHAVATVNGTAALHVALLAAGVTAGDEVLVPTFTFVATANAVAYCGAHPV
                     FLDCEETSWGLDPVRVAEFLDGECEVQGARVVNRRSGRTVRAMLPVHLYGHPCDLDPL
                     LALARRYPMAVVEDSAEALGARYRGRAVGADGLAGCLSFNGNKIITTGAGGMVLARDE
                     AMAARVRRLTTQARGDALEFIHDEIGFNYRLSNLHAALGVAQLEQIDGFVEDKRATAA
                     FYAGALGRLDGVSPFVEAAWARSTYWMASILLDERRCRDVRALIRDLNAAGIQARPLW
                     RPLHLQPAFQRAQTGPVSVAERLHARGLSLPCSVGITPEERQDVVDAVMQRLS"
     gene            complement(6786..>7120)
                     /locus_tag="VFX87_04265"
     CDS             complement(6786..>7120)
                     /locus_tag="VFX87_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HEX4994156.1"
                     /translation="RRAFCHVADALEAMTRLVSTEAAWGQVVNIGNDAEETRIGELGD
                     LILRTARFAPRVEQRPAPPGSVARRCPDIGRLRALTGFAPKVSLEAGVAETFAWYREW
                     SAREGAGR"
BASE COUNT         1083 a         2664 c         2520 g          853 t
ORIGIN      
        1 gggcgccggc atcgcgcgga tcgaagagca ggccgctctc gccgtcggcg aggatcgagc
       61 ggctcccgcc gacgttggag gcgacgcacg gcaccccgca ggccatcgcc tccagcagca
      121 ccttgggatg cccctccgtg aacgacgcca ggacgaagat gttggcggcc ccgtagacgt
      181 cgggaagctg ccgctggtcc acgaccccgg ggaaatcgac gaccacgccg agtctcttcg
      241 cctgggcctc cagatcgtct ctcaaggcgc ccgagcccac cagcgtgagc gtgacgtgtc
      301 caccggatcc ggcgccgatc gatccagctg cggtgacgac ggtgctcagg ttcttctccg
      361 ccgacagccg tcccacgtac aggatgtgcg tcggggtgcc ggccggccgg ctcaccgccg
      421 gccggaacag ggtggtgtcc acgccgttcg ggatcagctc tacccgccgc gccagccgcg
      481 acgcccgcgc ggccaggtcg ggcgtggtgg cgatgaccgc cgccgcgtgc ttgaggccca
      541 atcgctccac gagagccttc agtgctcgct tcggtcccgg ctgggagagc tgcgcatacc
      601 agaagccgta cgtggtcacg taggggatgc cgaagcggcg gcgggcgatc agcgccggga
      661 tcacgccggt gatctggaac acccgcagcg cggcgcactg ccgcatctcg gccgcgtgcg
      721 cgaccgggat ctgtaccgcc cggcggcccc gggccatcgg gcccggcgga gccaggacgc
      781 gcacgcgctc ccggagcatc gggtcggcgg tgaactccgc gagcgattcc ggcaggtagc
      841 tgaagtagtc gagcccgtcg aacgcgtcca ggtacgggcg gaagtacccg tcgatcagcc
      901 gcgactgctg tccgctctgg gccagcgcgc ggatgccgcc gcccagggcc ggcagcaggc
      961 ccacccgcat caggggcgct tcctggccac gacgcggaag ccggtgctct cctcgggccg
     1021 gcggagcgcg gcctcccgct tccagcggct gagccggtcg tagagggaca gcgagacgcc
     1081 gcccacggca tcgatgacac cggcccacgg ggtgtcgagg accaccggca tcaccgcgcc
     1141 ctccggctcg aagcccgcct cctggagctc ggcgaggaac tcctcgcgcg tgtactcgac
     1201 cttgtggtcg gggtccgaat acgagaagag cccggccgcg cgcagggtgc gccgccagga
     1261 gttctcccgg ttcgggccgg agaccaggag gcgcccgtcg tccttcagca cccggcggat
     1321 ctcgcgcagg accgccacgc gggggaccag gtgctcgatg acgtcgagaa agagcgcctt
     1381 gtcgaacgtg cggtcgggaa acgggaaggc ccccgtgatg tcccaggcca gcacgcgggc
     1441 ccgctcgagc cgctgctcct tgatggtccg gcccgcgatc gcaagctggg gcagatcgta
     1501 gtcgaagccg acgatggagc ggcacgcggt ggccgcgcgc accagatggg caccgttgct
     1561 gcagcccacg tccagcacga aatcgcggcg gtcgagatac ggcaggtacc agtcgtgcca
     1621 cggctgctcg accaggtgct tgggatggat cgcgtacggc cgcttgccgg tcagcttcac
     1681 cagccgcaca cccaccgact tgccccgccg gttgaccacg tccaccccgc gcacgagcag
     1741 gcgctgcagg ccgttcatcg cgcgcccttc ccgcccccgc ggtgacgcca ttcctcgagc
     1801 acggtctcgt agacgcggcg cgtgccgtcc acgcagcgct cccagcggaa catccgctcg
     1861 acccgctcgc ggttggcccg cccgagcttc tccccgaggg cccggtcccc caggatcagc
     1921 gccagcttct ccacgaaggg gccgggcccg gccgggtcgc agacgaagcc gccctcgccg
     1981 tccaccacca gctcgggaat ggatccgcgg ttggagacga ccaccggcag gcccgaggac
     2041 atggcctccg ccaccgtgag cccgaagcct tccatcgcgg aggggaagaa gaagacgtcg
     2101 cccaggttga agtgctcggg cttctcggcc tccgccacgt agccggtgaa caccacccgg
     2161 ccgtccagcc cgagccggcc cgcgtgggct cggagctcgg gcagcaggga gccgccgccc
     2221 gcgacaagca aggtggtgtc ggggtgccgg ggcgcgagca cccgccaggc ctcgaggagt
     2281 agccccaggt tcttccgcgg cttgagccca ccgaagaaga gcacgatccg gcggcccgtc
     2341 agcccgtagc gggcgcgcag cgcctcgctc ggcggccgcg gggtgaagcg cggatccacg
     2401 ccgtagtaga ccaccgagac gtggtcggtc cgcgccccga gctcctcgcg gagctgccgt
     2461 cgcgcgaact cgctgcccac caccacgcga tcggacgcgt tcaccacccg cttctcgatc
     2521 agccggttga gcgggctcgg atcgaggtgg tggtggtggg acacgacggg gacatccagg
     2581 ccgtaccatc gacgggcggc gagggcggac ggtccgatat agcgcaggga atgcacccgc
     2641 aacagatcga agcccaccat ctcgtagacg cgccggatgg ccgcgggcac cacgaacggg
     2701 gccacgtacc agcgcagacc gcgcccgata ccgaatcggt ggacggtcca gttcggcacg
     2761 tccggccggt gcggcttccc gcgggccagg atgatctcga ggcgcacccc cgcccggccg
     2821 agccgctcga gcagctcccg ctcgtaggtc tcgccacccg aggtggtctc gggggcgatc
     2881 ccgcagtggg gcgtgcagat cctcacgcgc gtctcacgag gcgggcggcc gctcggccac
     2941 gatcaggtag ccgcccacga aggcggcgaa gtcggactcg cgggcgcgcc gcagagagtc
     3001 caggcgcgtc aggagatcga gcagggggaa gaagccgtag ccgacccagc gcagcgggcg
     3061 cggcgcgcgc tgcaccagga agcgcagcat tcccgccagg tgcgtgaagt aaccaccctg
     3121 ctcggcgatg gtcaccggct tgaggtccgc ctctgccagc aggcgctccc acatgctgcg
     3181 ggtgtagcgg gcgtagtcac ctggatcgcc gtgcagacgc tccaggaagg gtacggtgat
     3241 cacgaggtgc ccgccgggac gcagcacacg ccggcactcg gccagcgccc gcgccacgtc
     3301 cggcacatgc tcgagcagct cggtggcctt gatcgcgtca aaggcgccgt cgcggaacgg
     3361 cagcgcctgc acgtcggcgc ccacgtgagg ccggtgcgta cgctcgatgt cggccaccac
     3421 ccagcgggcc gccggcggag ggcggaaggc gccgcgccga cgggccccgc ccacgtccag
     3481 cacccgcccg ctgaagtggc cggacacggc cgtcagatcg gcgtccacgc gcttgcgccg
     3541 gtaggacacg cccaggtagt acgcgaggcc ccggctcacg cgacggccgc cttgcgatag
     3601 acctcgagca gggcggtcac gagccgcgcc tcgtcgaagg cggtgcgggc gcgctcggcg
     3661 gcggcccggc ccatgcgggc gcggcgctcg ggatcgagca gcagctcgat gaccgccttg
     3721 gcgagcgcct ccgggtcctg ccgcggcacc aggaggccgg tcacgccgtg ctcgaccgcc
     3781 tccggggtcc ccccgaccgc ggtcgccacc accgggcggc cgatgcgcat gacctccagc
     3841 aggacgcggc tcagtggctc gggccacacc gagggcacca cgaccacgtc ggcggcgcgg
     3901 tagagcgcga acagctcggc ctgcggcagc acgcccagcc tccggagatc cggccgcgcc
     3961 gggatcgcca tgtcgccctt ccccgcgagc gcgaaacggg cgcccggcac cgccgcggcg
     4021 atgcggtcga gcgcgtccag cagcaccggc gtccccttcc cgatcgagag cttgcccgcg
     4081 tagagcacca ggggcgcggc ccccacgccg agccggtccc gcgcggcccg ggcctccgcg
     4141 tccgacggca ccgccaccgg cggcggaaga ttcggcacca cgcgagcgcg cggtcccagc
     4201 agccggatcg ggtacacgtc gagaatcccc tggctcacgc cgagcaggcc gtccgcgcgc
     4261 cgcatcgcct gctgacgcag ccggttgtcg atccacgcgg ccaccgcggc gacgcgcacc
     4321 cgcgcgcggc cgagcccgcc gagcccggcg gcgtagtgcg tcaggtggta ggggaggcac
     4381 ttgccgaggt actgcgtggc cgcgcagtcg aatcgctccc acggctcgaa caccgtgcag
     4441 tgaccgagcg gacacagcag tcccagatca cggatcgtta ccagcaccgg ccgaccgagg
     4501 cttcgaccgg cgagccagca gcccaccagg gccgccttgc cctgggcatg gatgatctga
     4561 gcccccgccg cgcgcgccgc gcgccgcacc tgccaggcga agtagagatg aaacagcggg
     4621 ttgcggtgca cgaggaagcg cacctcgccc tgtcccggac ggagcttcac cggaaacggc
     4681 acccggatga tcgtggcccc gtcgcgctcc tcgcgggccg ccgccccgta gttgggcgtg
     4741 accaccgtca cgcgatgacc gcgccgagtc agcgcggccg cccacgcggc ggtgctccac
     4801 tcggcgccgc cgggcgcgaa gggcggatag tactccgagg cgaacaggac cgacgtcatg
     4861 gcttgcgcgc caccacctcg atgaacggcg agaccccgga gagccgatag tagagcccga
     4921 tcggcacgcg cagcggcccc agcgactcgc ggggtgccgc gtcgtcgtga aagcggagat
     4981 cgaccacgcc cgcgcgcgcg aaggcccggc gcatcgctcg gggagtgagc cgccgcaggg
     5041 tcgcgaggta cccgctcggg cggccccggg cccgcaggta gagccgcccg agggcggcgg
     5101 gcaggaacgg cgcccacagg agcttgtagt ggccctcgta ccaggaccaa gcattgggag
     5161 tgtgcacgta gatgagaccc ccgcgacggg tcactcgcac catctccgcc accgattcct
     5221 cgaccgattc cacgtgctcg atcgccgaga agcaatacac gacgtcgaag gtgtcgcccg
     5281 ggaacggcag ccgctccgcg gcggcgcgga cgaaggtgcc cccgcccttc tcccgcttca
     5341 gcgcgcagat gccgatggcc tccgcgtcgg cgtccacccc gaccgcgcgc gcgccggctt
     5401 ccgccgccac caggttgaac ccgccggttc cgcagccgac gttcaggatg cggccccgca
     5461 cctccccgcc cagcagccgc cgcagccgat cgagccgccc gcgctcctgc cgctcctcct
     5521 cttctcggag tccgaccagc accgaccagt cggggatgcc caggacgtcg cggtagtagg
     5581 tggtgtagta gcgccggaga cggtcgcgca gctcgacagc gcggctcacg aaagccgctg
     5641 catgaccgcg tccacgacgt cctgccgctc ctccggcgtg atgccgaccg agcagggcaa
     5701 cgagaggccg cgcgcgtgga gccgctccgc caccgacacc gggccggtct gagcacgctg
     5761 gaacgcgggc tgcagatgga gcgggcgcca gagtggccgc gcctggatgc cggccgcgtt
     5821 gaggtcgcgg atcagggcac ggacatcgcg gcaccggcgc tcgtcgagca ggatcgaggc
     5881 catccagtac gtcgagcggg cccaggcggc ctccacgaac ggggaaaccc cgtcgagccg
     5941 tcccagggcg cccgcgtaga aggccgcggt ggcccgcttg tcctccacga agccgtcgat
     6001 ctgctcgagc tgggccacgc cgagggccgc gtgcaggttg ctcaagcggt agttgaagcc
     6061 gatctcgtcg tggatgaact cgagcgcgtc gccgcgcgcc tgggtggtga ggcggcgaac
     6121 ccgcgcggcc atcgcctcgt cgcgcgcgag caccatgccg cccgccccgg tggtgatgat
     6181 cttgttgccg ttgaacgaga ggcagcccgc caggccgtcg gcgccgaccg cacggccgcg
     6241 atagcgcgcg ccgagcgctt cggccgagtc ctccaccacc gccatcggat agcgccgggc
     6301 cagcgcgagc agcggatcga gatcgcaggg gtggccgtag agatgcaccg ggagcatcgc
     6361 gcggaccgtc cggccgctgc gccggttcac cacccgcgcg ccctgcacct cgcactcgcc
     6421 gtccaggaac tcggccacgc gcaccggatc gaggccccag gacgtctcct cgcagtccag
     6481 gaacaccggg tgcgcaccgc aataggccac cgcgttcgcg gtggccacga aggtgaacgt
     6541 cggcaccagc acctcgtcgc ccgcggtgac gccggccgcg agcagggcca cgtggagcgc
     6601 ggcggtgccg ttgacggtgg ccaccgcgtg cggcacgccc aggtactgtg ccacctcccg
     6661 ctcgaagcgg tccacgaagg ggcccgccga ggacacccag ttggtgtcga ggcattcctt
     6721 gacgtacttc cactcgttcc cggccaggtg cgggaccgcg ttcggaatca cgcgccgggg
     6781 cgcggtcacc gcccggctcc ctcacgcgcc gaccactcgc ggtaccacgc gaaggtctcg
     6841 gccactcccg cctcgagcga gaccttgggg gcgaagccgg tcagggcgcg gaggcggccg
     6901 atgtcggggc agcggcgcgc caccgagccg ggcggcgcgg gccgctgctc cacccggggc
     6961 gcgaagcgcg cggtgcgcag gatcaggtcc cccagctcgc cgatccgggt ctcctccgcg
     7021 tcgttgccga tgttcaccac ctgcccccac gccgcctcgg tggagacgag cctcgtcatc
     7081 gcctcgagcg cgtcggccac gtggcagaag gcgcggcgtt
//
LOCUS       DASUFA010000116         4647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1395212, whole genome shotgun sequence.
ACCESSION   DASUFA010000116 DASUFA010000000
VERSION     DASUFA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395212"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFX87_04270"
     CDS             <1..294
                     /locus_tag="VFX87_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994157.1"
                     /translation="DMKTTRVMSLVAATVFALGVAGSAVAQAPTTPAPATAPAPVQPT
                     PPAKGGDMQMEKKDKKDGKAAGKQHGKKRHAAAKKVEGDMKAAPETKAPEVKK"
     gene            complement(431..1810)
                     /locus_tag="VFX87_04275"
     CDS             complement(431..1810)
                     /locus_tag="VFX87_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4994158.1"
                     /translation="MTSGLPGNFAGRLAATARRRPDHPALIWDGGALSWRELEARAAG
                     LARRLARQGVGAGDVVALLLPNTWGLAAALWGGLALGATVAPLNPLLAADERERILGH
                     LGPKAVIDTAPDQESAPVSPIPDAAAPGIILYTSGSTGQPKGALLSHAALAVANESWA
                     GPVMGLAPDDRVLAALPLAHSFGLNGALLAPLLAGVTVVLQERFSPEEALGAIGRHRV
                     SVFPGVATMFARILESPALADADLSSLRLAVSGAAPCPWPLASEWRRRTGVRIVRGYG
                     MTELFRPISYLAAEPTDLPDSIGRAVPGVELRLVADELWIRSPAAMDGYLRAPEETAA
                     VLDGGWFKTGDLATISPEGFVSIVGRRRELILRGGYSVVPGEVEATLLGHPAVAEAAV
                     VGVPHAELGEEVAAFVTLRSRAATDPDELIAFCRARLAGYKYPRRITIVNELPKSATG
                     KILKGQLRE"
     gene            complement(1829..3880)
                     /locus_tag="VFX87_04280"
     CDS             complement(1829..3880)
                     /locus_tag="VFX87_04280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994159.1"
                     /translation="MTTTTPLLASGAHLVGSVPLASAETVFRGIAAEIGDRVRRIPDG
                     ETGPRSDWIVWQLPVFTTPRHLEVVPPGPDSWRPLPRVRLADGARPESVTFGALGYAD
                     AALSSYRVFARLKRDGVVPVGCRFQVCLPTPLAPISAFVVPEHQRVLEPVYEEQLLHE
                     LRLILQEIPHDQLAIQWDTNFEFGMLEGIFPVWFDDVKGGILERLLRLSRHVPSDVQL
                     GYHFCYGDVQHRHFKEPADTSKLVDVANALGASLGRPLNWVHLPVPRDRFDEAYYAPM
                     RELRLRSETELYLGLVHHTDGVEGTRRRMDIARRFVSGFGVATECGWGRRPPDTIAAL
                     LRIHAAVSAPIAQAGGPRRRFEWPSGFGRVPDQDWTLQPVDTFGLRYDTVENHGWYRN
                     LDPTVEDLARHLKDGDLLIDYSGGTGILLDRLNLRIFDRQVGTLIVDSSPKFLRVALD
                     KFGDEERVAFRLLRWRKEEKRLDLLDEVVGPELLARTADAIASTNAIHLYRDLPETLA
                     SWARVLRPGGRVFVQSGNIRNPQARPSEWILDETVWAIHEVATGLVRNDPRYAAYRPL
                     LDDEPRMRAHVAYRDQVFLPVRPLDHYLRCLEEAGFTVEAVTARSIEAQVGHWFEFLS
                     AYHEAVLGWLGTSVKVDGKAPSSETVTDRLALIRHAMDTLFGGREAFRCCWTYINCVR
                     P"
     gene            complement(3885..>4647)
                     /locus_tag="VFX87_04285"
     CDS             complement(3885..>4647)
                     /locus_tag="VFX87_04285"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEX4994160.1"
                     /translation="PAIALGALAIGSIDEDERAIMGALLREPLELVQCETSDVLAPAQ
                     AEMIIEAEILPGARTPEGPFGEFTGYSLGERAREVVQVKAITHRRGAYFQDITVAHLD
                     HMLLSTIPMEANLYRAVRAMVPSVKAVRVPAPFTCYVSIEQRLIGQAKNAILSVLGAD
                     LYMKRVVVVDEDVDIFDDRQVTWAIATRCQPDRDITIISNARGSDLDPSSREDGYSAK
                     WGVDATAKPSLAGYTPRHRVPPAVWKRLNLKDYVE"
BASE COUNT          699 a         1613 c         1723 g          612 t
ORIGIN      
        1 gatatgaaga cgacccgtgt gatgtctctc gtggcggcaa cggtgttcgc gctcggcgtg
       61 gccggctcgg cggtggctca ggccccgacg acccccgccc cggcgacggc tcccgcgccc
      121 gtgcagccca cgccccccgc gaagggcggc gacatgcaga tggagaagaa ggacaagaag
      181 gacggcaagg cggccggcaa gcagcacggc aagaagcggc acgccgcggc caagaaggtt
      241 gagggcgaca tgaaggcggc tcccgagacg aaggcgcccg aagtcaagaa gtaggcgcaa
      301 cgaagtagga agcgcccagc cgcgcgaggg atcgcgcgtt cacataggcg cggcggcgcg
      361 gggggagcac cctcgcgccg cctgctcccc tttctccctc tcctcttcgg ggagaacgca
      421 ggccgagggc tcactcccgt agctgcccct tcaggatctt cccggtcgcg ctcttcggta
      481 gctcgttcac gatcgtgatc cgccgcggat acttgtagcc ggccagccgc gcgcggcaga
      541 acgcgatcag ctcgtccgga tcggtggcgg cgcgcgaccg caacgtcacg aacgcggcca
      601 cctcctcgcc cagctcggcg tgcgggaccc cgaccaccgc agcctcggcc accgccgggt
      661 ggccgagcag cgtcgcctcc acctcgccgg gcaccaccga gtagccgccc cgcaggatca
      721 gctcgcgccg gcgtcccacg atgctcacga agccctcggg cgagatggtg gcgaggtcgc
      781 cggtcttgaa ccagccgccg tcgagcaccg cggcggtctc ctcgggtgcg cgcaggtagc
      841 cgtccatggc cgcgggcgag cggatccaca gctcgtcggc caccaggcgc agctcgacgc
      901 cgggcaccgc gcggccgatc gagtccggca gatcggtcgg ctcggcggcc aggtaggaga
      961 tgggacggaa gagctcggtc atcccgtagc cccgcacgat ccgcaccccg gtgcgtcgcc
     1021 gccactcgct ggccagcggc cacgggcagg gcgccgcgcc ggagactgcg aggcgcaggc
     1081 tcgagagatc cgcgtccgcc agcgcgggcg actcgaggat gcgcgcgaac atcgtggcca
     1141 cgccggggaa cacgctgacc cggtgccggc caatcgctcc gagcgcctcc tcgggcgaga
     1201 agcgctcctg cagcaccacc gtgacgccgg ccagcagcgg ggccagcagc gcgccgttca
     1261 gcccgaacga gtgggcgagc ggcagggcgg ccagcacgcg gtcgtccggc gcgaggccca
     1321 tcaccggccc ggcccacgat tcgttggcga ccgcgagcgc ggcgtgggag agcagcgcgc
     1381 ccttgggctg cccggtgctg cccgacgtgt agaggatgat gccgggcgcg gcggcgtccg
     1441 ggatcggcga taccggggcg ctctcctggt cgggtgcggt gtcgatgacc gccttcgggc
     1501 cgaggtgccc gaggatgcgc tcgcgctcgt cggcggccag cagcgggttg agcggggcca
     1561 cggtggcgcc gagcgcgagc ccaccccaga gcgcggcggc caggccccac gtgttcggca
     1621 ggagcagggc gaccacgtcg cccgcgccca cgccctggcg cgcgagccgc cgcgccaatc
     1681 cggccgcgcg cgcctcgagc tcgcgccagg aaagcgcgcc gccgtcccag atcagtgcgg
     1741 gatgatccgg ccgccggcgc gccgtggcgg cgagccgccc ggcgaagttc ccagggaggc
     1801 cggatgtcac ggtggccggc gtggagtctt acggccggac gcagtttatg taggtccagc
     1861 agcagcggaa cgcttcgcga cctccgaaca gcgtgtccat cgcgtgccgg atgagggcca
     1921 gccggtcggt gacggtttcg ctgctgggcg ccttgccatc gaccttgacg gaggtgccca
     1981 gccagcccag cacggcttcg tgatacgcgc tcaggaactc gaaccagtgc ccgacctggg
     2041 cctcgatgga gcgggccgtg accgcctcga cggtgaagcc ggcctcttcc aggcagcgga
     2101 ggtagtggtc cagcgggcgg acgggcagga agacctggtc ccgataggcc acgtgcgcgc
     2161 gcatgcgcgg ctcgtcgtcg agcagcgggc ggtaggccgc atagcgcggg tcgttgcgga
     2221 ccagtccggt cgcgacctcg tggatcgccc acacggtctc gtccaggatc cactcgctgg
     2281 gccgggcctg ggggttgcgg atgttcccgg actgcacgaa cacgcgccca cccggccgca
     2341 gcacccgagc ccaggaggcc agggtctccg gcaggtcccg gtagagatgg atcgcgttcg
     2401 tggacgcgat ggcgtcggct gtccgggcca gcagctccgg tccgacgacc tcgtcgagga
     2461 gatcgagccg cttctcctcc ttgcgccagc gcaggaggcg gaacgcgacg cgctcctcgt
     2521 ccccgaactt gtcgagcgcg acgcggagga acttgggcga gctgtcgacg atcaacgtgc
     2581 cgacctgacg gtcgaagatt cgcaggttca accgatcgag caggatgccg gtcccccccg
     2641 aatagtcgat cagcaggtct ccgtccttca ggtgacgggc cagatcctcc acggtcgggt
     2701 cgaggttgcg gtaccagccg tggttctcga ccgtatcgta gcgcaggccg aaggtgtcga
     2761 ccggctgcag cgtccagtcc tggtcgggca cgcggccgaa gccggagggc cactcgaacc
     2821 gccggcgcgg cccgccggct tgcgcgatcg gggcgctgac cgcggcgtgg atgcgcagga
     2881 gcgcggcgat ggtgtcgggt ggccgccggc cccagccgca ctcggtggcg acgccgaagc
     2941 cggacacgaa acgccgggcg atgtccatgc gccggcgggt tccctcgacg ccgtcggtgt
     3001 ggtggaccag cccgaggtag agctcggtct cggagcgcag gcgcagctcc cgcatcggcg
     3061 cgtagtacgc ctcgtcgaag cggtcgcgcg ggaccgggag gtggacccag ttgagcgggc
     3121 ggccgaggct cgcgcccagc gcgttggcca cgtcgaccag cttcgacgtg tcggccggct
     3181 ccttgaagtg gcggtgctgg acgtctccgt agcagaagtg gtaaccgagc tggacgtcag
     3241 acggcacgtg gcgggagagg cgcagcagcc gctcgaggat cccacccttg acgtcgtcga
     3301 accagaccgg gaagatcccc tccagcatcc cgaactcgaa gttggtgtcc cactgaatgg
     3361 cgagctgatc gtgcgggatc tcctggagga tcagccgcag ctcgtgcagc aattgctcct
     3421 cgtagaccgg ctccagcacg cgctggtgct cgggcaccac gaacgcgctg atgggggcga
     3481 gcggggtggg caggcacacc tggaagcgac agccgaccgg caccacgccg tcacgcttga
     3541 gccgggcgaa gacgcgatag gaggacaggg ccgcgtccgc gtacccgagg gccccgaagg
     3601 tcacgctctc cggccgcgcg ccgtcggcca ggcgcacccg gggcaggggc cgccagctgt
     3661 cgggcccggg cggcacgacc tcgagatggc gcggggtggt gaagacgggg agctgccaga
     3721 cgatccagtc ggatcgaggc ccggtctcgc cgtccgggat gcgccggacg cgatccccga
     3781 tctcggcggc gatgccgcgg aagacggtct ccgcgctggc cagcggcacg ctgccgacca
     3841 gatgcgctcc ggatgccaag agcggggtcg tggtcgtcac gttgctactc cacgtagtcc
     3901 ttcaggttga ggcgcttcca caccgcgggc ggcacgcggt gccgcggggt gtagccggcc
     3961 agcgagggct tggcggtcgc gtccacgccc cacttcgcgg agtagccgtc ctcgcgggac
     4021 gaggggtcga ggtccgagcc gcgcgcgttg gagatgatcg tgatgtcgcg gtccggctgg
     4081 cagcgggtgg cgatcgccca ggtgacctga cggtcgtcga agatgtccac gtcctcgtcc
     4141 accaccacca cgcgcttcat gtagaggtcg gcgcccagca cggacaggat cgcgttcttg
     4201 gcctggccga tcagccgctg ctcgatcgag acgtagcagg tgaagggcgc gggcacccgc
     4261 accgccttca ccgaggggac catggcccgg accgcgcggt agaggttggc ctccatcggg
     4321 atcgtggaga gcagcatgtg gtcgagatgg gccaccgtga tgtcctggaa gtaggcgccc
     4381 cgccggtggg tgatggcctt gacctgcacg acctcgcgcg cgcgctcgcc caggctgtag
     4441 ccggtgaact cgccgaacgg gccctcgggc gtccgcgcgc cgggcaggat ctccgcctcg
     4501 atgatcatct cggcctgcgc gggggccagc acgtcggagg tctcgcactg caccagctcg
     4561 agcggctcgc gcaggagcgc gcccatgatc gcgcgctcgt cctcgtcgat ggagccgatg
     4621 gcgagggcgc ccagcgcgat ggccggg
//
LOCUS       DASUFA010000117         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1058671, whole genome shotgun sequence.
ACCESSION   DASUFA010000117 DASUFA010000000
VERSION     DASUFA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058671"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFX87_04290"
     CDS             <1..283
                     /locus_tag="VFX87_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX4994161.1"
                     /translation="KRNGLKMDDIGVWELNEAFASQVVYCRDKLGIPMEKLNPNGGSI
                     SIGHPFGMTGSRMVGTIANEMLRRKARYGVVTMCIGGGQGAAGLFESCL"
     gene            379..1536
                     /locus_tag="VFX87_04295"
     CDS             379..1536
                     /locus_tag="VFX87_04295"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HEX4994162.1"
                     /translation="MSYDFDRVIDRRATSSNKWRKYPADVLPLWVADMDFASPEPVVR
                     ALRERVEHGVYGYGFEEPEFAQVFVDRLQKRYGWKVSPEAVVMIPGVIPGFNVACHAL
                     TKPGDGLLMQVPVYPPILRAPGNHGLTRDEAPLARDRDGRYAADLDAFRAAIHGRTRA
                     FLLCNPHNPVGRVFTREDLAGMAQICLERDLAIIADEIHCDLLYRGQRHIPMASLGPE
                     IEQRTITLMAPSKTFNLAGLKVSVAVIPNASLRERFMAARGDYVQAQVNVLGYAAAFA
                     AYRDGDAWLGELLRYLEANRDYLAEYVRRHLPGVTMTPPEGTYLGWLDCRGAGAAAAD
                     PFTFFLERAKVAFNDGALFGRGGQGFVRLNFGSPRSILTEGLERMRRALAG"
     gene            1698..2042
                     /locus_tag="VFX87_04300"
     CDS             1698..2042
                     /locus_tag="VFX87_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994163.1"
                     /translation="MRGGWRATVVAAAMLCAGCATAPAGPSVMVLPGTGRPFAEFQAD
                     VSLCRDWAAKQVKGAFMDAPSFEVQRRYDNAYIQCMYAKGHQVPGRDLPARTPPPPAG
                     SAPPPPPQTPPK"
     gene            2050..>4203
                     /locus_tag="VFX87_04305"
     CDS             2050..>4203
                     /locus_tag="VFX87_04305"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX4994164.1"
                     /translation="MTETIMINIFKRLIASLLALILFVGAAPLARAASPAVPAPPAPP
                     AAAAPEDGEADDSQNLAPTPPRLSYINGDVSFWRPGGEDWAPAQVNTPLAPGDQLYTG
                     NRGNLEVQIDSRSFVRAWGDSQLGLENHEPDFLQLKVAAGHVSLDLRALDSGRTVELA
                     TPAAAFTIARPGYYRVDVAPDRTSFVTRRGGTASMTPAGGQAVRIAASEQVVLTGNGT
                     ASVQTFVAPEQDVWDRWNYARTEALVDTVSARYVPPTVSGVDDLDQHGNWRVVPEYGS
                     VWVPEGVGTDWAPYTTGRWVWDPRFGWSWVDTAPWGWAPYHYGRWVHLDRVWAWAPGP
                     VVVRPVYAPALVAFFGVPGVSVTIGVPFVSWVALGWGEPCVPWWGRPGFVGRAWWGGW
                     GGPRVVNNVVINRTTVVNVTNINVYRNVSVQNAVVAVRPDRFGRGPVGEARIRQVDTR
                     QLQPVRGAVDVRPDRSSFAAASGRGVRPPENVLDRGVVATRAPVTRALPREPEGRGPA
                     PRIVPAPRPNGVGEARPPLGQSGLERARPPRAPRFESGQRPSATPPAARREDPAAQER
                     ARRPEAQRVPDPQRAPGSPRAAEPQRAPEVPRGPEPQRAPQVQQAPQVQRPPQVQRAP
                     EAPVERRAPADVPRQPRRETVDQPRAVERPTAPVPQPRPVERPAAPAPQPRQLERPPA
                     AGPQPRQQQRVLPGEPANRLYPGGRVEAPKPRAPES"
BASE COUNT          576 a         1550 c         1499 g          578 t
ORIGIN      
        1 caagcgcaac ggcctcaaga tggacgacat cggcgtgtgg gagctgaacg aggccttcgc
       61 ctcgcaggtg gtctactgcc gcgacaagct gggcatcccg atggagaagc tcaacccgaa
      121 cggcggctcc atctcgatcg gccacccgtt cggcatgacc ggctcgcgca tggtcggcac
      181 catcgccaac gagatgctgc ggcgcaaggc gcgctatggc gtcgtgacca tgtgcatcgg
      241 cggcggtcag ggcgccgcgg gcctcttcga gtcctgtctg taatctcctc gcggtgatct
      301 catcccctcg ccctcaaccc tctcccctcc ggggagaggg cagggtgacg ggtgacgctt
      361 ccccccaggt gatctgagat gtcctacgac ttcgaccgcg tcatcgaccg ccgcgccacc
      421 agcagcaaca agtggcggaa gtatcccgcc gacgtgctcc cgctctgggt cgccgacatg
      481 gacttcgcct cgccggagcc cgtggtgcgg gcgctgcgcg agcgggtcga gcacggcgtg
      541 tacggctacg ggttcgagga gcccgagttc gcccaggtgt tcgtggaccg cctgcagaag
      601 cgctacggct ggaaggtgtc ccccgaagcg gtcgtcatga tcccgggcgt gatccccggc
      661 ttcaacgtgg cctgtcatgc cctcacgaag ccgggcgacg ggctcctgat gcaggtgccg
      721 gtctatcccc cgatcctccg ggcgccgggc aatcacgggc tcacccgcga cgaggccccg
      781 ctcgcgcgcg accgcgacgg ccgctacgcg gcggatctcg acgccttccg cgcggccatc
      841 cacggccgca cccgcgcgtt cctgctctgc aacccgcaca atcccgtggg ccgcgtcttc
      901 actcgagaag acctcgcggg catggcgcag atctgcctcg agcgggacct ggcgatcatc
      961 gccgacgaga tccactgcga cctgctctac cggggccagc gccacatccc gatggcctcc
     1021 ctcggccccg agatcgagca acgcacgatc acgctgatgg cgcccagcaa gacgttcaac
     1081 ctggccgggc tcaaggtctc ggtggcggtc attccgaacg cgtcgctgcg cgagcgattc
     1141 atggccgcgc gcggcgacta cgtgcaggcc caggtcaatg tcctcggcta cgccgcggcc
     1201 ttcgcggcgt atcgcgacgg cgacgcgtgg ctcggcgagc tgctgcgcta cctcgaggcc
     1261 aaccgggact acctggccga gtacgtgcgc cggcacctgc ccggcgtcac gatgacaccg
     1321 cccgagggaa cctatctcgg ctggctcgat tgccggggcg cgggcgccgc cgcggcggac
     1381 cctttcacgt tcttcctcga gcgcgcgaag gtggcgttca acgacggggc gctcttcggc
     1441 cggggtggcc agggcttcgt ccgtctcaac ttcggctcgc cgcggtcgat cctgaccgag
     1501 gggctggagc ggatgcgccg ggcgctcgcc ggctgagcct cgactgatct tagacgcgcc
     1561 ccgtcccggg ggcgtttaca ctagatttct cgctcgtttc ttccgacggg gtcccttccc
     1621 cgatacgaat gtccccggac ggccctcgtc tgactgcatg taacccggaa gcccggccca
     1681 gcacccagga ggtcgcgatg cgtggaggat ggagagcgac ggtggtggcg gcggcgatgc
     1741 tgtgcgcggg ctgcgcgacc gcgcccgcgg gcccgagcgt gatggtactg ccgggcaccg
     1801 gccggccgtt cgccgagttc caggccgacg tgagcctctg ccgcgactgg gccgcgaagc
     1861 aggtgaaggg cgccttcatg gacgcgccgt ccttcgaggt gcagcgccgc tacgacaacg
     1921 cctacatcca gtgcatgtac gccaaggggc atcaggtgcc gggacgcgac cttcccgccc
     1981 ggacgccacc cccgccggcg gggtcggccc cgccgcctcc tccgcagacg ccacccaagt
     2041 gacgcccaag tgacggagac catcatgatc aacatcttca agcgactgat cgccagcctc
     2101 ctggcgttga ttctgttcgt gggggccgcg ccgctggcgc gcgccgcctc gcccgccgtt
     2161 cccgctccgc cggcgccccc ggcggccgcc gctcctgagg acggggaagc cgatgatagc
     2221 cagaaccttg cgccgacgcc gccccggctc agctacatca acggcgacgt atcgttctgg
     2281 cgccccggcg gcgaggactg ggcgcccgca caggtgaaca ccccgctggc ccccggcgac
     2341 cagctctata ccggcaaccg cggcaacctc gaggtgcaga tcgactcccg gagcttcgtg
     2401 cgggcctggg gcgacagcca gctcggcctc gagaaccacg agcccgattt cctccagctc
     2461 aaggtggcgg ccggccacgt gtcgctggac ctgcgcgcgc tggacagtgg acgcaccgtg
     2521 gagctcgcca cgcccgccgc cgcgttcacc atcgcccggc cgggctatta ccgcgtggac
     2581 gtggcgcccg atcggacgtc tttcgtcacc cggcgcggcg gcaccgcgag catgacgccg
     2641 gccggcggcc aggcggtgcg catcgcggcc agcgagcagg tggtgctcac gggcaatggc
     2701 acggcctccg tgcagacctt cgtcgcgccc gagcaggacg tctgggatcg ctggaactat
     2761 gcgcgcaccg aggccctcgt ggacaccgtg agcgcgcgtt acgtgccgcc gacggtctcc
     2821 ggcgtggacg acctcgacca gcacggcaac tggcgcgtgg tgccggagta cggctcggtg
     2881 tgggtgccgg agggcgtcgg gacggactgg gccccctaca ccaccgggcg ctgggtctgg
     2941 gatccgcgct tcgggtggag ctgggtggac acggcaccct ggggctgggc gccgtatcac
     3001 tacggccgct gggtgcacct ggaccgcgtc tgggcgtggg cgccggggcc cgtggtcgtg
     3061 cgaccggtct acgcgcccgc gctcgtcgcc ttcttcggcg tgcccggcgt gagcgtcacc
     3121 atcggcgtgc ccttcgtgag ctgggtcgcg ctcggctggg gcgagccgtg cgtgccgtgg
     3181 tggggccgtc cgggattcgt cggccgcgcg tggtggggtg gctggggcgg gccgcgtgtg
     3241 gtcaacaacg tggtcatcaa ccgcaccacg gtggtcaacg tgaccaacat caacgtgtac
     3301 cggaacgtca gcgtgcagaa cgcggtggtg gcggtgcggc cggaccgctt cgggcgcggg
     3361 cccgtcggcg aggcgcgcat ccgccaggtg gacacgcgcc agctccagcc ggtgcgcggt
     3421 gcggtggacg tccggccgga ccgctcgagc ttcgcggcag cgagcggccg cggcgtccgt
     3481 ccgccggaga acgtgctgga tcgtggcgtc gtcgcgaccc gggcgccggt gacccgggcc
     3541 ctgccgcgcg agccggaagg ccgcgggccc gcgccgcgca tcgtgccggc gccaaggccg
     3601 aacggcgtgg gtgaagcacg gccgccactg ggtcagagcg gtctcgagcg ggcgcgtccg
     3661 cctcgggcgc cgcgcttcga gagcggccag cgtcccagcg ccacgccgcc cgcggcgcgg
     3721 cgtgaggatc cagcggcaca ggagcgggcc cggcgacccg aagcccagcg cgtgcccgat
     3781 ccgcaacgag cgcccggatc accgcgggcc gcggagccgc agcgcgcgcc ggaggtgccg
     3841 cgtgggcccg agccccagcg cgcgccgcag gtccagcagg cgccgcaggt ccagcgcccg
     3901 ccacaggtcc agcgcgcgcc ggaagcaccg gtcgagcgcc gcgcgcccgc ggacgtcccg
     3961 cgccagccgc ggcgtgagac ggtcgaccag cctcgcgccg tggagcggcc cacggcaccc
     4021 gtaccgcagc cgcgcccggt cgaacggccg gccgcgcccg cgccacagcc gagacagttg
     4081 gagcggccgc ccgccgccgg cccgcagccg cgccagcagc aacgcgtgct ccccggtgag
     4141 ccggccaacc ggctgtatcc aggcggtcgt gtggaagccc ccaaaccgcg ggcgcccgaa
     4201 tca
//
LOCUS       DASUFA010000118         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_486186, whole genome shotgun sequence.
ACCESSION   DASUFA010000118 DASUFA010000000
VERSION     DASUFA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486186"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFX87_04310"
     CDS             <1..543
                     /locus_tag="VFX87_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4994165.1"
                     /translation="PLDGTTNYAHGLPIYCVSIALELDGDLAVGVLYDPSRDECFVAE
                     RGRGATLNGAPLRVSATPTLGESLLGTSYPNDLRSAPRNNLAEHAALIQRSRSVRSLG
                     SAALGLAAVAAGRLDGYWEQRLGAWDIAAGALLIREAGGTVTAVHGAPLDLAAPTIAA
                     SNGPIHSDLLQALKEVAAAQ"
     gene            540..1130
                     /locus_tag="VFX87_04315"
     CDS             540..1130
                     /locus_tag="VFX87_04315"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEX4994166.1"
                     /translation="MTEETKTPPVHVRTIRVEVARAGEHELEITATLVDERPHDNPRW
                     FGAEPPRVIHDMRLGLRVRHPDLVITEARSEMASHPYTICPDALPPLQQLVGLSVSRG
                     FTREINERFGRQRGCAHFTALIHAVAPAVRQGAGVAFADCERPAEGAPWFVNTCQAWR
                     ENGPLHRLIEAGDEGGLRGLSAYPPGPEAPSRPGTG"
     gene            complement(1085..1576)
                     /locus_tag="VFX87_04320"
     CDS             complement(1085..1576)
                     /locus_tag="VFX87_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX4994167.1"
                     /translation="MSPPPTPDPSAWTLPKLRIDRDGLWFHDDAEVTHPGVIANLREN
                     LQVDERGHYLQIGPARVPVEVEDTPYVVERVEATGAELTATLNDLTREPVALDTLRVD
                     ERGIPRCRVKEGRFDARLSRAATYQLLQHARPDEGAGRASLVLGGRRYPVPGLEGASG
                     PGG"
     gene            complement(1573..2841)
                     /gene="amrB"
                     /locus_tag="VFX87_04325"
     CDS             complement(1573..2841)
                     /gene="amrB"
                     /locus_tag="VFX87_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX4994168.1"
                     /translation="MTRAGNPRLRALEAFPVEQQGRRCVALRDPAGFTDQIAVLPPPL
                     LDLVSLFDGDHSIEEIREILQRRHGKAPTLRQIADIVERFDAAGLLDSDRFRERRRAL
                     EDAFRQGPVRPAAHAGGAYAGDAAALRAQIDGFFSASDGPGTAISGDSSTDPVASPSA
                     AAPQARTRALIAPHIDFHRGGSTYAWAYREILERSDADLYVVLGTCHAGMPDPFAVTL
                     KAYETPLGPVPVDREFYDALNRRAGQDLLASEPAHRAEHSIEFQAVMLQHLVGPRRPF
                     AILPVLASYLHESLWSGADPEADPRIPRFVDALRETMAASSRRVCLVAGVDLAHVGPR
                     FGDQEPNSAAFLARVERDDRAMLQSVVEADARGFYAGIAADHDARRICGLSPIYTLLR
                     LLPEAPGRLVRYTQWPDPQGAVTFCAVAYP"
     gene            complement(2838..4070)
                     /locus_tag="VFX87_04330"
     CDS             complement(2838..4070)
                     /locus_tag="VFX87_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX4994169.1"
                     /translation="MTALSQVLRKIDADEVVELTRSLVRIPSVYRPGDPEATEIRVAA
                     FVEAWLRREGFQVEVQQVAPGRPNIIGWLGEKRAGGKSLLLEGHTDVVTEGDPAEWSH
                     PPFGADLVDGRIYGRGTADMKSGLAAAMVAAAAFKRAGVALRGKLVVGALVDEEDGMI
                     GVRHLCGTAVGREVDAAIICEPEENELCLEQRGVVWARFRVRGKMAHGAMPQAGVNPI
                     AGLGRLLAAAPGLERRLRKACEKSRYLKPPTVTPTIIQGPPAGVGVPQSNVIPALAET
                     TFDIRLTAGIGAEGIRAELEAICRAAAAAQPGIKVEWEPVNGFRLATKVDRSEALVGA
                     MVRGVKQATRRAAKYGGVPGSTDGTILRMELGIPIVTCGPGDRLIPHQVDEFVRADEV
                     VEAAKIYAASALNYLESE"
     gene            complement(4087..>4388)
                     /locus_tag="VFX87_04335"
     CDS             complement(4087..>4388)
                     /locus_tag="VFX87_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994170.1"
                     /translation="PDIQFQVYGINLLVRLMVEPPADVRVHCPKGSPIRYGEVVGRGD
                     GFDEGANAFRDMPPIRSVVAFEENPEEVEGHYFYIAGEEHRVIRLDAIILSFPLE"
BASE COUNT          623 a         1624 c         1559 g          582 t
ORIGIN      
        1 ccgctcgacg gcaccaccaa ctacgcccac gggctgccga tctactgcgt gtcgatcgcg
       61 ctcgagctgg acggcgatct ggcggtcggc gtcctctacg atcccagccg cgacgagtgc
      121 ttcgtggccg agcgcggccg cggggccacg ctgaacggcg cgcccctgcg cgtctccgcg
      181 acacccacgc tcggtgagag tctcctcggc accagctacc cgaacgacct gcggtccgcc
      241 ccgcgtaaca acctcgccga gcacgcggcc ctcatccagc gcagccgcag cgtgcgcagc
      301 ctgggctccg ccgcgctcgg actcgcggcc gtcgcggccg gccgcctcga cggttactgg
      361 gagcagcggc tgggcgcgtg ggacatcgcg gcgggcgccc tgctgatccg cgaggcgggc
      421 ggcaccgtga ccgccgtgca cggggccccg ctcgacctgg cggcgcccac catcgcggcc
      481 tcgaacggcc cgatccattc cgacttgctc caagccctca aggaggtcgc ggccgctcaa
      541 tgaccgagga gacgaagacc ccgccggtgc acgtgaggac catccgcgtc gaggtcgcgc
      601 gggcgggaga gcacgagctc gagatcaccg caaccctcgt ggacgagcgg ccgcacgaca
      661 acccgcgctg gttcggcgcc gagccgccgc gcgtgatcca cgacatgcgg ctcggcctgc
      721 gcgtgcgtca ccccgacctg gtcatcacgg aggcgcgtag cgagatggcc tcgcacccct
      781 acacgatctg tccggacgcc ctgccgccgc tccagcagct cgtgggcctg tccgtctcgc
      841 gcggcttcac acgggagatc aacgagcgct tcggccggca gcgcggctgc gcccacttca
      901 ccgcgctgat ccacgcggtg gccccggcgg tgcgccaggg ggcgggcgtc gccttcgcgg
      961 actgcgagcg gcccgcggag ggggcgccgt ggttcgtcaa cacctgccag gcgtggcggg
     1021 agaacggccc gctgcaccgc ctgatcgagg cgggcgacga aggggggctg cgcgggctct
     1081 ccgcctaccc gcccgggccg gaagcgccct cgaggcccgg caccgggtag cgccgcccgc
     1141 ccagcacgag actcgcccgg ccggccccct cgtcgggccg cgcgtgctgg aggagctgat
     1201 aggtcgcggc ccggctcagc cgcgcgtcga agcggccctc cttcacgcgg cagcgcggga
     1261 tgccccgctc gtccacgcga agcgtgtcga gcgccaccgg ctcccgcgtc aggtcgttga
     1321 gcgtggccgt gagctcagcg cccgttgcct ccacgcgctc gacgacgtac ggcgtgtctt
     1381 ccacctccac cggcacccgc gcgggcccga tctgcagata gtggccccgc tcgtccacct
     1441 ggaggttctc gcgcaggttg gcgatgacgc cggggtgtgt gacctcggcg tcgtcgtgaa
     1501 accacaggcc gtcgcgatcg atgcgcagct tcggcagggt ccaggccgag ggatcgggcg
     1561 tggggggcgg gctcacgggt aggccaccgc gcagaaggtc accgcgccct gcgggtccgg
     1621 ccactgggtg tagcgcacga gacggcccgg ggcctcgggc aggaggcgca gcagcgtgta
     1681 gattggcgag aggccgcaga tgcggcgggc gtcgtggtcg gcggcgatcc cggcgtagaa
     1741 gccgcgcgcg tccgcctcca ccaccgactg cagcatggcg cgatcgtccc gttcgacccg
     1801 ggcgaggaac gcggcgctgt tgggctcctg gtcgccgaac cgggggccca cgtgggccag
     1861 gtccacgccc gcgaccaggc atacccgccg cgaggacgcc gccatcgtct cccgcagcgc
     1921 gtccacgaag cgcgggatgc ggggatccgc ctcgggatcg gcgccgctcc acagcgattc
     1981 gtgcaggtac gaggcgagca ccggcaggat ggcgaagggc cgccggggcc ccacgagatg
     2041 ctggagcatc accgcctgga actcgatcga gtgctccgcg cggtgcgccg gctcggaggc
     2101 cagcaggtcc tgcccggccc gccggttgag cgcatcgtag aactcgcggt ccaccgggac
     2161 gggcccgagc ggcgtctcgt aggccttgag cgtcaccgcg aacgggtccg gcatgcccgc
     2221 gtgacaggtg cccagcacca cgtagaggtc cgcgtccgag cgctcgagga tctcccggta
     2281 cgcccacgcg taggtcgagc cgccgcgatg gaagtctatg tgcggagcga tcaacgcccg
     2341 agttcgagcc tggggagccg cagccgaagg cgaggcgacc ggatcagtag aggagtcacc
     2401 gctgatcgcg gtacccggcc cgtccgacgc ggaaaaaaac ccgtcgatct gcgcccgcag
     2461 cgccgccgcg tccccggcgt aggcgccgcc cgcgtgtgcg gcgggacgca ccgggccctg
     2521 ccggaaggcg tcctcgaggg cccgacgccg ctcccggaag cggtcgctgt cgagcaaccc
     2581 ggcggcatcg aatcgctcga cgatgtcggc gatctgcctg agggtgggcg ccttgccgtg
     2641 ccggcgctgc aggatctccc ggatctcctc gatcgagtgg tctccgtcga agagcgacac
     2701 caggtccagc agaggtggcg ggagcacggc gatctgatcg gtgaagccgg cgggatcacg
     2761 cagcgccacg cagcgccgcc cttgttgctc gaccgggaag gcctcgaggg cccggagcct
     2821 cgggttaccg gcgcgggtca ctcggattcg aggtaattga gagccgacgc ggcataaatc
     2881 ttcgccgctt cgaccacttc atcggccctg acgaactcgt cgacctggtg ggggatcagc
     2941 cggtccccgg gcccgcaggt gacgatcgga atgcccagct ccatccggag gatcgtgccg
     3001 tccgtcgagc ccggcacccc accgtacttt gcggcccggc gcgtcgcctg cttgacccca
     3061 cggaccatcg ccccgaccag ggcctcggag cgatccactt tggtagccag gcggaacccg
     3121 ttcaccggct cccactccac cttgatcccg ggctgggcag cggccgcggc acggcagatc
     3181 gcctccagct cggcgcggat gccctcggcg ccgatgcccg cggtcagccg gatgtcgaac
     3241 gtggtctcgg ccagcgcggg gatgacgttc gattgcggca cgcccacgcc ggccggcggc
     3301 ccctggatga tggtcggggt caccgtcggc ggcttgagat agcggctctt ctcgcaggcc
     3361 ttccgcagcc gtcgctcgag gccgggcgcc gcggccagca gccggccgag cccggcgatc
     3421 ggattcacgc ccgcctgggg catcgccccg tgagccatct tgccgcgcac gcggaagcgc
     3481 gcccagacca cgccgcgctg ctcgagacag agctcgttct cctccggctc gcagatgatc
     3541 gcagcatcca cctcgcggcc caccgcggtg ccgcagaggt ggcggacgcc gatcatgccg
     3601 tcctcctcgt ccacgagggc gccgacgacc agcttgcccc gcagcgcgac cccggcccgc
     3661 ttgaacgcgg cggcggcgac catggcggcg gccagcccgc tcttcatgtc ggcggtgccg
     3721 cggccgtaga tgcgaccgtc caccaggtcg gccccgaagg gcgggtgcga ccactcggcc
     3781 ggatcgccct cggtcaccac gtcggtgtgc ccctcgagca gcaggctctt accaccggcc
     3841 cgcttctcgc cgagccagcc gatgatgttc gggcggccgg gcgcgacctg ctgcacctcc
     3901 acctggaagc cctcgcgccg gagccacgcc tcgacgaagg cggccacgcg gatctcggtg
     3961 gcctcggggt cgccggggcg atagacgctg gggatgcgga cgagcgagcg ggtcagctcc
     4021 acgacctcgt ccgcgtcgat cttgcggagg acctgcgaca gcgcggtcac agcgggagcg
     4081 gggcgcctac tcgagcggga acgagaggat gatcgcgtcg agccggatca cgcgatgctc
     4141 ttccccggcg atgtagaagt agtgcccttc cacctcttcg gggttctcct cgaaggccac
     4201 caccgagcgg atcggcggca tgtcgcggaa ggcattggcc ccctcgtcga agccgtcgcc
     4261 acggcccacc acctcgccgt agcggatcgg cgagcccttc gggcagtgca cgcgcacgtc
     4321 cgccgggggc tcgaccatga gccgcacgag gaggttgatg ccgtacacct ggaactggat
     4381 atcaggca
//
LOCUS       DASUFA010000119         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1275689, whole genome shotgun sequence.
ACCESSION   DASUFA010000119 DASUFA010000000
VERSION     DASUFA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275689"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VFX87_04340"
     CDS             <1..1478
                     /locus_tag="VFX87_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4994171.1"
                     /translation="IARGAAVYGDAPAVIQGARTLSFRALQGRVDELAAGLAGLGLGV
                     GDRVCVLAQNDAAYLELYGACARQGLIAYPINWRLTGQEVERVVERARPAMMVVDAST
                     QALVGDWLGGAGVTHRYHLGEGPGAGFTAFASLYRKGAAPAPADVAADAPFAVISTAA
                     VDVVPRGAVLSHANVIAANLTTIGCLGFTAQDRYLVALPLFHVSALAMARAHMHAGGA
                     SVIVSRFDPAEAVALIDRHRVTHVSDFPPVLASLLDAAEKAGSTLPSLTHVSGLDAPQ
                     TIQRLHERTKAQFWTGFGQSETSGFVTIQRVAERPGASGRPVPAAQVRLVDDYDREVP
                     VGTPGEIVVRGPLVFQGYFGQADVTAYTFRTGWHHTGDVGRFDADGYLHYVKRKPEKE
                     LIKPGGENVYPAEVEAVIMQMAGVTGVCVYGIPDAKWGEAVKAVVEVAAPGAPTAQQV
                     TDFVGGKIARFKRPHVVAFCEALPRTAEGAIDRDAVKAKWA"
     gene            1483..>2189
                     /locus_tag="VFX87_04345"
     CDS             1483..>2189
                     /locus_tag="VFX87_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994172.1"
                     /translation="MRIFEPHHDMFRQAVRAFVQKEVEPHVEQWEQAGEIPKSIWPRM
                     GALGFLGVEYDEKYGGAGADVLTTAVLCEEMARARCASLAMAVGVHTDMASPHLYWTG
                     SEALKEKYLPAICRGEALTAIAVTEPGGGSDVAAIRTRAVRDGDHYVLDGSKMFITNG
                     VLADIYFVAARIEQGTGDRRHRGISMFLVERNTPGFTVSRKLEKMGNRASDTAELAFQ
                     GMRVPAGNLLGTEGKGF"
BASE COUNT          314 a          747 c          823 g          305 t
ORIGIN      
        1 tgatcgcgcg gggcgccgcg gtgtacggcg acgcgcccgc ggtcatccag ggcgcgcgca
       61 cactatcgtt ccgggcgctc caggggcgcg tggacgaact ggccgccggg ctggccgggc
      121 tggggctcgg ggtcggcgac cgggtctgcg tgctcgccca gaacgacgcg gcctacctcg
      181 agctctacgg ggcctgcgcg cggcaaggcc tcatcgccta tccgatcaac tggcggctca
      241 ccgggcagga ggtggagcgg gtggtggagc gggcgcgacc cgccatgatg gtcgtcgacg
      301 cttccacgca ggccctggtc ggcgactggc tcggcggcgc cggggtgacc catcgctacc
      361 atctcggcga ggggcccgga gccgggttca cggcgttcgc gagtctctat cgcaagggcg
      421 ccgcgcccgc tcccgcggac gtggccgccg atgccccgtt cgcggtgatc tcgaccgccg
      481 cggtggacgt ggtgccccgg ggggccgtgc tcagccacgc caacgtgatc gccgccaatc
      541 tcacgacgat cggctgcctc ggcttcacgg cgcaggaccg gtacctggtc gccctgccgc
      601 tctttcacgt gagcgcgctc gccatggccc gggcccacat gcacgcgggg ggcgccagcg
      661 tgatcgtgtc gcgtttcgac ccggcggagg cggtggcgct catcgaccgt caccgcgtga
      721 cccacgtgtc cgacttccct ccggtgctgg cgagcctgct cgacgcagcg gagaaggcgg
      781 gcagcacgct cccgagcctg acccacgtct ccggcctcga cgcgccccag accatccagc
      841 gcctgcacga gaggacgaag gcgcagttct ggaccggctt cggccagtcc gagacgagcg
      901 ggttcgtgac gatccagcgc gtggccgagc ggcccggcgc ctccgggcgg ccggtacccg
      961 ccgcccaggt gcgcctcgtg gacgactacg accgcgaggt accggtcggc acgccggggg
     1021 agatcgtggt gcgcggcccg ctggtcttcc agggctactt cggccaggcc gacgtcaccg
     1081 cctacacctt ccgcaccggc tggcaccaca ccggggacgt gggccgcttc gacgccgacg
     1141 gctatctgca ctatgtcaag cgcaagccgg agaaggagct gatcaagccg ggcggggaga
     1201 acgtgtaccc ggcggaggtc gaggcggtga tcatgcagat ggccggcgtc accggcgtct
     1261 gcgtctacgg gatccccgac gccaagtggg gtgaggcggt caaggcggtg gtggaggtgg
     1321 ccgcgccggg ggcgcccacc gcccagcagg tgaccgactt cgtgggcggc aagatcgcgc
     1381 gcttcaagcg gccgcacgtg gtagcgttct gcgaggccct gccgcggacc gcggaggggg
     1441 cgatcgaccg cgacgcggtc aaggccaagt gggcgtgacg ccgtgaggat cttcgagccc
     1501 caccacgaca tgttccggca ggcggtgcgg gcgttcgtgc agaaggaagt ggagccgcac
     1561 gtcgagcagt gggagcaggc gggcgagatc ccgaagtcca tctggccgcg gatgggcgcg
     1621 ctcggctttc tcggcgtgga gtacgacgag aagtacgggg gcgccggggc cgacgtgctc
     1681 accaccgcgg tgctgtgcga ggagatggcc cgggcgcgct gcgcctcgct cgcgatggcg
     1741 gtgggggtgc acaccgacat ggcctcgccg catctctatt ggacgggcag cgaggccctg
     1801 aaggagaagt acctgcccgc gatctgccgg ggagaggcgc tcaccgcgat cgcggtgacc
     1861 gagccgggcg gtggctccga cgtggccgcg atccggaccc gcgcggtgcg cgatggcgac
     1921 cactacgtgc tcgacggctc caagatgttc atcaccaacg gagtgctggc cgacatctac
     1981 ttcgtggccg cgcgcatcga gcaggggacc ggggatcggc gccatcgcgg catctcgatg
     2041 ttcctggtgg agcggaacac ccccggcttc acggtgagcc ggaaactcga gaagatgggc
     2101 aaccgcgcct ccgacaccgc cgagctcgcc ttccagggca tgcgggtgcc cgccgggaac
     2161 cttctcggca ccgagggcaa gggtttcta
//
LOCUS       DASUFA010000120         3088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1397198, whole genome shotgun sequence.
ACCESSION   DASUFA010000120 DASUFA010000000
VERSION     DASUFA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397198"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFX87_04350"
     CDS             complement(<1..511)
                     /locus_tag="VFX87_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4994173.1"
                     /translation="MSIRANKVKRRLAAGQVATILSGTNDPDLIDQLGPVGADGIWLE
                     GEHGGVDYADLGNLTRACDLWGVTSVVRVMDNDYATIYRTLDRGAQGIVVPHVNTRAE
                     AQAVVEGAKFAPIGKRGMFTSRQGFGVDDYLKTANDQSLLIVLIEDIVAVHNLDAILT
                     VDHIDVFFVA"
     gene            complement(534..1358)
                     /locus_tag="VFX87_04355"
     CDS             complement(534..1358)
                     /locus_tag="VFX87_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994174.1"
                     /translation="MLAELREDDATGEIAGIYDQIRRLWAVPYVSSLQRHLATRPGWL
                     EWAWAALGPAFTSGIAQAAAWRAADGLAVPRLAPISRDALAVWSVDAAGEAAIRATCA
                     SFVRVSPVNLVLSGLLRRLLTGERPPGANGGPRAWTPPASLGPLPALADPAALPPAQR
                     AVLATLGTTVAGQVFVPGLYRMLASWPAFLAHVATVLRPHLDDAATRAAGQRLLAAVD
                     AEIPRVFATLPALPARPPMPPAAEFAEVLAALDTYRKTSPEMVVFGRMLGDALPRP"
     gene            complement(1367..3007)
                     /locus_tag="VFX87_04360"
     CDS             complement(1367..3007)
                     /locus_tag="VFX87_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4994175.1"
                     /translation="MTDLHALSIADLARLIAARKLSPVELTEALIQRVEQHDAQTHAF
                     ITPTFDLARRQARQAEAEIAAGRARGPLHGIPFGLKDIYDTAGILTSAHSRVFIDRIP
                     TEDATATTRLYEAGAVLLGKLATHEMAHAGPSFDLPWPPARNPWNLAHFTGGSSTGSG
                     AAVAAGFLPMALGSDTGGSIRGPASHCGVVGIVPTFGLVSRAGVITNSYTFDHCGPLT
                     RTVQDGALTLQALAGYDPRDAGSLRRPIPRFDEALGQGLRGLRIGVLRHHWEQDIPAS
                     DDVRKAMEASLDVLRRLGAELEDCRVRALGAYYDVKIIIAETEIFSVHQRDLIARPGD
                     FGADFRARALPAVLFTANDYVQATREHRRMMVEMEPLYDRFDAFVTAGLGEAPRLADH
                     RSVSFWQKPSGLTAWNVTGQPVLALPNGFGRNGLPLGMQILGRPFGEAAILRIGHAYE
                     QATEWHRRRPALVAGAEAPAVTPPPVLSVTDQADAATRDLCATAARRAGLRLDDQMLA
                     QLLEGAPYALGMVERLRRDHGLHHEPANIFSFPASQPR"
BASE COUNT          424 a         1055 c         1194 g          415 t
ORIGIN      
        1 gggccacgaa gaagacgtcg atgtggtcca cggtcaggat cgcgtcgagg ttgtgaaccg
       61 ccacaatgtc ctcgatcagg acgatgagca ggctctggtc gttggcggtc ttgaggtagt
      121 cgtcgacccc gaagccctgg cggctcgtga acatgccgcg cttgccgatc ggggcgaact
      181 tggcgccctc caccaccgcc tgggcctccg cgcgggtgtt gacgtgcggc acgactatcc
      241 cttgcgcccc gcgatccagc gtccggtaga tcgtggcgta gtcgttgtcc atcacgcgca
      301 ccacgctggt cacgccccag agatcgcagg cccgcgtgag attgcccaga tcggcgtagt
      361 ccacgccgcc gtgctcgccc tcgagccaga tgccgtcggc gcccaccggg ccgagctggt
      421 cgatcaggtc cggatcgttg gtgcccgaca ggatggtggc cacctggccg gcggccagcc
      481 gccgcttcac cttgttcgct cgaatgctca tggggtcgtc ctctcgtctc tggtcagggt
      541 ctgggcaggg cgtctccgag catgcggccg aagacgacca tctcgggact ggtcttgcga
      601 tacgtgtcga gcgcggccag cacctccgcg aactcggcgg cgggcggcat gggaggccgc
      661 gcgggcagcg ccggcagggt ggcgaacacc cgggggatct cggcgtccac cgcggccagg
      721 agccgctggc ccgccgcgcg cgtggccgcg tcgtcgagat gcggccgcag caccgtcgcc
      781 acgtgggcga gaaaggccgg ccacgaagcc agcatccggt agagccctgg gacgaacacc
      841 tggccggcca cggtggtgcc gagcgtggcc agcacggcgc gctgcgcggg cggcagcgcg
      901 gcgggatcgg cgagggcggg cagcgggccc agggatgccg gcggagtcca ggcgcgcggg
      961 ccgccgttgg cgcccggcgg gcgctcgccg gtgaggagcc ggcgcagcag acccgacagc
     1021 acgaggttga ccgggctcac gcggacgaag ctcgcgcagg tcgcgcggat ggccgcctcg
     1081 ccggccgcgt ccacgctcca caccgcaagc gcgtcacgcg agatcggcgc cagccgcggc
     1141 accgcgaggc cgtcggcggc gcgccaggcc gcggcctgcg cgatcccgct cgtgaaggcg
     1201 ggcccgagcg cggcccacgc ccactcgagc cagccgggcc gcgtggccag gtggcgctgg
     1261 agcgaggaga cgtatgggac ggcccagagg cgccggatct gatcgtagat gccggcgatc
     1321 tcgccggtgg catcgtcctc tcgcagctcg gccagcatcg cggcgcctag cgcggctgag
     1381 aagccgggaa gctgaagatg ttcgcgggct cgtggtgcag gccgtgatcc cggcggaggc
     1441 gctcgaccat gcccagggcg tagggcgcgc cctcgaggag ctgggccagc atctgatcgt
     1501 cgagccggag ccccgcgcgc cgggcagcgg tcgcgcagag atcacgggtg gccgcgtcgg
     1561 cctggtcggt gaccgagagc acgggcgggg gcgtgaccgc gggtgcctcg gcgccggcca
     1621 ccagcgcggg gcgccggcga tgccactcgg tggcctgctc gtaggcgtgg ccgatccgca
     1681 ggatggccgc ctcgccgaag ggacggccga ggatctgcat gcccagcggc aggccgttgc
     1741 gcccgaatcc gttgggcagc gcgagcaccg gctggccggt gacgttccac gcggtgagcc
     1801 cgctcggctt ctgccagaac gagacgctgc ggtggtcggc gagccgcggg gcctccccca
     1861 ggccggcggt gacgaaggcg tcgaaccggt cgtagagcgg ctccatctcg accatcatgc
     1921 ggcggtgctc gcgcgtggcc tgcacgtagt cgttggcggt gaagagcacg gcgggaagcg
     1981 cgcgggcgcg gaagtcggcg ccgaagtcgc cggggcgtgc gatcaggtcg cgctgatgga
     2041 cgctgaagat ctccgtctcc gcgatgatga tcttgacgtc gtagtaggcg ccgagcgcgc
     2101 gcacgcggca gtcctcgagc tccgccccga ggcgccgcag cacgtcgagc gacgcctcca
     2161 tcgccttccg cacgtcgtcc gacgccggga tgtcctgctc ccagtggtga cggagcaccc
     2221 cgatgcgcag cccgcgcagg ccctgcccca gcgcctcgtc gaagcgcggg atcggccgcc
     2281 gcaggctgcc cgcgtccctc gggtcgtagc cggccagggc ctgcagggtg agcgcgccgt
     2341 cctggacggt gcgggtgaga ggaccgcagt ggtcgaacgt gtaggagttg gtgatcacgc
     2401 cggcgcggct caccaggccg aacgtgggca cgatgccgac caccccgcag tgggatgccg
     2461 gcccccggat cgacccgccg gtgtcggagc cgagcgccat cgggaggaac ccggccgcga
     2521 cggccgcccc cgagccggtg gaggacccgc cggtgaagtg ggcgaggttc caggggttgc
     2581 gcgcgggcgg ccacggcagg tcgaacgagg gacccgcgtg ggccatctcg tgggtcgcca
     2641 gcttgccaag caggacggcg ccggcctcgt agagcctggt ggtcgcggtg gcgtcctcgg
     2701 tcgggatgcg gtcgatgaag acgcgtgaat gcgcggaggt gaggatgccc gccgtgtcgt
     2761 agatgtcctt gagcccgaac gggatgccgt gcagtggccc gcgcgcccgg cccgccgcga
     2821 tctcggcctc ggcctgccgc gcctgccgcc gggccaggtc gaaggtgggc gtgatgaacg
     2881 cgtgcgtctg ggcgtcgtgc tgctcgacgc gctggatcag cgcctcggtc agctccaccg
     2941 gcgacagctt gcgcgcggcg atcaggcggg caaggtcggc gatcgagagg gcgtggaggt
     3001 cggtcatggc ggaatctcag agcgtcagct tggtcttccc gtcgatgtcc accagcatcg
     3061 agtccgggac gtacccgacg ctgatcgt
//
LOCUS       DASUFA010000121        24083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_831357, whole genome shotgun sequence.
ACCESSION   DASUFA010000121 DASUFA010000000
VERSION     DASUFA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 24083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24083
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831357"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..901
                     /locus_tag="VFX87_04365"
     CDS             143..901
                     /locus_tag="VFX87_04365"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4994176.1"
                     /translation="MGKLEGKVALISGGARGQGAAEAETFTREGAKVVFGDIRDDEGK
                     KVEAAIRAAGGDATYLHLDVTSEADWKAAVQTAIDRYGKLDILINNAAIVIPRVPIEE
                     RTVAEWDQVMAVNAKGVFLGTKYAIPAMRRAGGGSIVNISSIAGIGQSLHQEPAYAAS
                     KGAIRIFTKVTASQHAKDKIRCNSVHPGPVDTEMFHSAFRDKEAMARRLQRIPLGRMG
                     TVAEIVSAVLYLASDDSSYMTGSEVLIDGGALAQ"
     gene            complement(1007..1852)
                     /locus_tag="VFX87_04370"
     CDS             complement(1007..1852)
                     /locus_tag="VFX87_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX4994177.1"
                     /translation="MPKVLTESQVRSFERDGCLSPVRVMSAERARHYRERFEALEARV
                     PDIKKMKTKSHLLCPWVLEIAEDPHILDVFEDLIGPNIRCWSMAWRVKKADGQTFAGW
                     HQDSAYGAAIPVVLGGLALSECGVSQGCLRGIPGSHKWGILKHDESEDPHSILSKGQF
                     ITDPFDETKAVDFALEPGEMVLFDNTLVHGSGTNAGPDRRFLLLVEMFPTWAKPPRVR
                     QAGMLMRGIDTSGNFDDEPRPDAEWSATAVANWSAVVSRRARLIFEDSKVAPSEAYGG
                     TRAAT"
     gene            1968..3512
                     /locus_tag="VFX87_04375"
     CDS             1968..3512
                     /locus_tag="VFX87_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994178.1"
                     /translation="MSYWRTAVVLLSLVLVPLTAGAAPAGRVVIAQGVDPTTLDMMNQ
                     QEQPASNVGAQMFESLIERDQNLKLVPALAAEMPKLVAPTTWEFKLRRGVKFHNGEDF
                     TADSVKFSIERLVNPANKLRGSSSFAPIDRVEIVNPFTVRIHTKKPWPVFLNHIAQRQ
                     AAMYPPKEYAGKDAAAISRAPIGTGPYKFVRWSKDEEIVMEAFPSYWGGAAKIKTVVF
                     KPIPDDAVRVAALQNAEIDVAVNIPPHMAGIIEKHPKIFLSTAPSIRTIQLMIYTHQM
                     DAAHKPTGPYALPTADKRVRQALSYAIDADEIIKGVMDGKAVRVATMLPSMHFGFDPS
                     LKPVKQDLARSKKLLTEAGFPNGLEIVLNGPQGRYVRDKEVAEAVAGQLTKAGIKTTL
                     RTFEFVNYLNNMVYVHKAGPVWLIGWGHPTMDAEAIYVPLFKSPNIFVNWHNEDFNGM
                     VEQAQSEMDEKKRLAMYHRINKLWIEEVPAIPLYQQIDLYGANKRLTWKARSDELIRA
                     YDMAIK"
     gene            complement(3566..4597)
                     /locus_tag="VFX87_04380"
     CDS             complement(3566..4597)
                     /locus_tag="VFX87_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4994179.1"
                     /translation="MRYRPFGRTGLKVSVVGFGCWPMAGDRYGAIEDDEAVKAIHRAL
                     DRGVNCVDTAPAYGAGHSEEVVARALAGQRQEVILVTKCGVKAPPPGQPGPLRDASRT
                     NILAEIDASLKRLRTDWVDVLLVHWPDAKTPFDESMRALEEVVASGRARFVGVSNFTG
                     AMLAECMRTRRVDVSQVGYHMLDRRQEQETFPYCVSHGIGVMGYGSLGHGLLTGAFTA
                     TTTFDPARDWRAGGVAFGQPIFRGDNLKTNVGVVERLRREVAEPRGVPLSQIALAWVL
                     ANPAISTALVGARTPAEVDANDAGAELELSAAERATIDRILGGVAGRVREFTPLRPAM
                     EPWGQELTA"
     gene            complement(4600..5739)
                     /gene="alr"
                     /locus_tag="VFX87_04385"
     CDS             complement(4600..5739)
                     /gene="alr"
                     /locus_tag="VFX87_04385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX4994180.1"
                     /translation="MPSGPLRPTTLEIDLDAAADNLRAVRRLVGPSRKIFAVVKADGY
                     GFGAAEMGAVFVRNGADYLAVADLTEGVRLRRRGIATPILVYPNSLPGAAAETLAHGL
                     VPTLVDLEAARVYSAAATGPCEVFVKIDVGLERLGVPAEQAVKLIVAMLELRHLRLGG
                     LCAHPHAEVGGDPAYADWQLGRFTGVVDELEARGIRVPVRLLAASPFVLRFPHTYLNA
                     VDPGRMLYGITFPGETPPVPLRSALRALTTRVIALKELAPRERFAEAAPFPVTAPLRL
                     GVVPMGSADGLRWLHAGRVLVRGRAVPLIGAPSLEHTRIDLTGVPDAAVGDEVVIIGR
                     QGDLEITSAEVASRHGLGLHHVATTVGPRVARVYLEAGAAAPLEG"
     gene            complement(5750..6517)
                     /locus_tag="VFX87_04390"
     CDS             complement(5750..6517)
                     /locus_tag="VFX87_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4994181.1"
                     /translation="MRPNKIKQMWRENKCVTMGWLSVSNGFTAEVMARQGFDALVIDM
                     QHGLTDMANLWPMLQAVSQTDTVPVVRVASNDPAAIMKALDLGAYAILVPLINTAEDA
                     AKAVAACRYPPVGMRSSGPVRAVHYGGADYVARANDEIVVMAMIETKEGLANLDAICA
                     TPGLDCIYIGPADLSFALGLAPRGDNPDPVHIATCDRIRDAAHKHGKKAAMHCASAAF
                     AAGSVKRGFDLVMLCSDLASMVAGVRKQLDDLKAATA"
     gene            6590..7381
                     /locus_tag="VFX87_04395"
     CDS             6590..7381
                     /locus_tag="VFX87_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994182.1"
                     /translation="MDLELADKVAIVGGASKGLGRACAEVLAQEGCRVAICSRSRDDL
                     EKAAKDIHGATGREVLVFAGDLDRPETVRGLVAATVERFGRLDILVNNSGGPPLAQAQ
                     TASEEQWATAVERSLFFFARMCREALPHLKRQAAGRIINILASTVYQPIPNLVLSGVT
                     RMGVVAFAKTLADEVGRDGVLVNNVCPGSIMSERMLSNVTARATQLGITLEEALAQRA
                     AETAVGRLGEPRELAHLVAFLASGKSSYITGTTILVDGGLVRSVL"
     gene            7412..8707
                     /locus_tag="VFX87_04400"
     CDS             7412..8707
                     /locus_tag="VFX87_04400"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4994183.1"
                     /translation="MSHTRRDLLKGAAALAAAATVSAGEATMSRQAHAQGSAGPARAR
                     IDGVLRQAVDAKEVPGVVAMAATDQGVLYEGAFGPRALEPGAPAMTMDTVFRIASMTK
                     VITSVAAMQLVEQGKLKLEEPVPNIDPSLGAPQVLDGFDAAGAPKLGAANRPITLRHL
                     LTHTAGFSYDIWDASTGRYVKASGMPARATAKVASIRTPLIFDPGDRWEYGVNTDWVG
                     RLVEAASGQPLDAYFRDKIFAPLGMKDSGYVTSTEQRARQARLHLRQADAALAPQPLE
                     AVPVAGEFWSGGGPLYSTGRDYIEFLQMLLHGGSWKGARVLKPETVALMQQNHTGSIP
                     AGILKTQMPALSNDVDLFPGAQIRWGLGYMLNLQPGPNGRSAGTVSWGGIFNTYYWLD
                     PAKRVTGLIMTQILPFADHRALKLYGQFESAVYEALPKA"
     gene            complement(8709..9773)
                     /locus_tag="VFX87_04405"
     CDS             complement(8709..9773)
                     /locus_tag="VFX87_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4994184.1"
                     /translation="MDRLRLAIVGCGNISRLNAPGYLQHPRCDVVALCDTRPERAKRR
                     ATEWGITPRLCSDFAQVLDDPAVDAVELLTPTWMHADQIVAALDAGKHVSCQKPLAIT
                     VAEADRIAAAVARARTTFRVTENFLYYPPIVKAKALLDAGAIGEPSLVRIHTTRAQRV
                     EGITLDMEPDAMVWRRDPARNPGGVLFDDGVHKYGTAAYWIGEIGEVSAIVTRGPDFI
                     METPSVATWRFEGRDCLGIMDYTYAPEMAIRGRYVRVDEFFEIHGSRGIIWVTRCSGE
                     MLDLPPVQVIRGTETVSYQVPMDWRLGFDGAAADFVDGVLDGRQPAQDVQAARHMLQV
                     SLAIYESGRTGHAVRPDSIA"
     gene            complement(9787..10335)
                     /locus_tag="VFX87_04410"
     CDS             complement(9787..10335)
                     /locus_tag="VFX87_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994185.1"
                     /translation="MPRIVPITGKSDVPAAHHGVVDQVVKVFGGVRGPFSMLLHSPQL
                     AERLLPLVTFFRDESAVEGKLRSVAILAAVREREAAYVWSAQVGQARRNGVAEAIIDV
                     LRAKGDPSGLPADEREIIGYVRQLMRTNKVDQAAFDALAKRHDPRWLVEMTAAASYFA
                     MLCGVVNAFEVAAPPDGDKLPA"
     gene            complement(10358..11653)
                     /gene="aroA"
                     /locus_tag="VFX87_04415"
     CDS             complement(10358..11653)
                     /gene="aroA"
                     /locus_tag="VFX87_04415"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX4994186.1"
                     /translation="MAETPAPVRPVQPITQPFDAEVALPGSKSYSNRALLVAALARGR
                     SQITQALWSDDTRYMHRALEALGVRVTADEAARAFAVEGVDGRFPAAEATLDIGNAGT
                     AARFLTAAVALGQGTFVVDGSPAMRKRPIQPLLDGLRALGVDAESREGTGCPPVVVRA
                     KGIAGGRARMRGDISSQYFSAILLAAPYARHDVEIEVEGELVSAPYITMTLSTMEAFG
                     VRAEREGDRRFRVPAGQRYEARAYAVEPDASAASYFFAAAAVTGSRVVVPRLGRLSAQ
                     GDLGLVDVLARMGCEVAVEDDSITVRGPARLLAVDADFTRMGDVATTLMAIAPFADGP
                     VTVRGIAQTHFEESDRPVAAATELRRMGLQVESAWDSVTIHPGTPRPTDVQTYDDHRI
                     AMSFAVTGLRAPGIRIVNPACVSKTFPEYFDVLRGLTNG"
     gene            11716..12591
                     /locus_tag="VFX87_04420"
     CDS             11716..12591
                     /locus_tag="VFX87_04420"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4994187.1"
                     /translation="MAITIRQVGPCFAGEVNGLDLARPLSPEDAVAIHAGMDRYAVLV
                     FHGRPVTPDQHMAFTLALGAIENAVGTGLRENYRLPTVFADVSNLDKDERVFARDNRT
                     RLFGLGNRLWHSDSSFKPIPAKYSLLHAYRIPSRGGNTEFADMRAAWDALDAETQAAC
                     EGLICEHSQLYSRQILGFTDFTDEERERFAPVRQVLVRTHPVTGRKSLYLSSHAGGII
                     GWPVPEARAFLRDLVEHATQRQFVYSHRWQVGDLVMWDNRQTMHRARPFPQHEPRDVR
                     RTTLAGDRPTVAQVS"
     gene            complement(12584..14065)
                     /locus_tag="VFX87_04425"
     CDS             complement(12584..14065)
                     /locus_tag="VFX87_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4994188.1"
                     /translation="MPAARPLVLVFVVDGLRPDSITAEDTPTLFRLRAEGVDLVNGHA
                     VFPTVTRVNAAAIATGTQPGTNGLVGNQMFVPAVDERRAFDTGNHRNFLALDRASDGR
                     VLEVPTLAERLHARGLVLAGVSSGSTGSAFLLNPRAPAGVGALVNGYLDPGHTVAYPA
                     GVSESILSRLGPAPVKAPGGGRFDAQVNWTQRVLREYVLPELGPAVVINWLTEPDHSQ
                     HHVGVGSPSAREAIRNDDREIARVLATLDALGLTPATSVLVVSDHGFTTNTAGVDVAG
                     ALVAARLKAAPDSTDVILASSGQAVAIHVEGHDAERIARLARFIQAQDWGGVVFAAGP
                     VEGAFPLELINVANRDRSPDLLFTFPWTSRRNAFGVPGTDWANVSAGGAPYASDHGSM
                     SPWNVRNTFLAWGAGVKKGVTVRAPAGNVDVTPTALALLGLDGEAGLDGRVVTEALEG
                     GPDPEQVAVETRVHTAAAGAYRAALQISTVDGRRYVDKSWRVS"
     gene            complement(14065..14211)
                     /locus_tag="VFX87_04430"
     CDS             complement(14065..14211)
                     /locus_tag="VFX87_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994189.1"
                     /translation="MDHQKVDDRLWAEVRAQFSEAEVIELVAHTTLYIGFGRFNEIVG
                     IDPA"
     gene            complement(14344..14583)
                     /locus_tag="VFX87_04435"
     CDS             complement(14344..14583)
                     /locus_tag="VFX87_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994190.1"
                     /translation="MARMQPLPMHDLDPEIQHMCRESEAQIGTSASTRTYARNPAVVK
                     ALAAFRGALVREGTIGPVIRELVRIKIAGLNACRY"
     gene            complement(14604..14921)
                     /locus_tag="VFX87_04440"
     CDS             complement(14604..14921)
                     /locus_tag="VFX87_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX4994191.1"
                     /translation="MVLVGNLAQVTGGVAEFDLSAASVQQLFRQLGELHPALAPHLEN
                     GVAVAIDGQIFQDALLEPIGANSEVFLLPAIAGGAGVRSYIPTFPRATLSVAGPKMSE
                     CKT"
     gene            complement(14942..17245)
                     /locus_tag="VFX87_04445"
     CDS             complement(14942..17245)
                     /locus_tag="VFX87_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4994192.1"
                     /translation="MGETNRASTSNGTNGQHYVGTRTIRPDGVDKVTGRARFGADFNL
                     PGQLIGRVLRSPHPHARIVSIDTSKAEALPGVKAVITRDDFADQPSEFIPAGEMMMNY
                     KDVVRNVMAREKALYEGHPVAAVAATTTAIARRALKLIEVKYEILPHVIDVVEAMRPG
                     APLLHDDLFTAGVEPRPDKPSNIAKRVEIKLGDVEAGFKKADVIIEREFKTAPVHQGY
                     IEPHAALATVSEDGATELWTTTQGHYIVRAHCARLLGLDMGKVRVTATEIGGGFGGKT
                     VVYLEPLAIALSRKAKRPVKMVMSREEVFKASGPTSGATVRVKMGATKDGKFVAGAAE
                     LKYQAGAFQGSPVQPGVMCAYAPYDIENVHVIGYDVVSNRPKVAAYRAPGAPISEYAV
                     ESVVDEIAKKLRLDPIELRMMNAAREGTKAAYGPKFGPIGLEETLNAIKKSEHWNTPL
                     RKWQGRGVASGFWFNIGGETTVSLTVNEDGTVSLMAGTPDIGGLRASLASVVADELGV
                     SYDKVRPIIGDTGSLGFNFLTGGSRSTFSGSMAIVEAAKQIKADLCARACKLWEAKPD
                     QVEWKDGAVRPLGALAEKVKPMTVEDFAKTAGKTGGPIGGVGRINAQGAGPSFGTHLV
                     DLEVDPETGRATILRYTVAQDAGRALHPAYVEGQYQGGAVQGIGWALNEEYIYGADGK
                     LQNAGFLDYRMPVASDVPMIDAILVEVANPKHPYGVRGVGETPIVPPLGAIANAMENA
                     TGIRFTELPMSPPKVLKALKEKGLSKA"
     gene            complement(17250..17726)
                     /locus_tag="VFX87_04450"
     CDS             complement(17250..17726)
                     /locus_tag="VFX87_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009818588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4994193.1"
                     /translation="MAKHHIEATVNGDPVEFLCETEDTLLDVLRDTLDLTGSKEGCSS
                     GDCGACSVMLDGRLVCSCLVLGCEVGGRKIETIEGLAKGESLHPLQRRFLEAAALQCG
                     ICTPGFLVASKALLEKNPNPSEHEVRFWLAGNLCRCTGYDKIVTAVMDTAAILRKG"
     gene            complement(17726..18586)
                     /locus_tag="VFX87_04455"
     CDS             complement(17726..18586)
                     /locus_tag="VFX87_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX4994194.1"
                     /translation="MRYEAPESLEGAVALLAGAKGEARVLAGGTDLLVQMRADMVDPE
                     LIVDIKKIPETRTVTEEKGGWRIGAAVTGAELKEHARLKKVWPGVVEAANLIGSTQVQ
                     GRATLGGNLCNGSPAADSVPALIAAGALATLAGPKGRRDLPVEDVMLGPRKLALTKGE
                     IVVSFLLPPRPARSSDAYLRFIPRTEMDIAVVGAGVSLTLDAGGTIIAARVSLGAVAA
                     RVLLVAEAAQAIIGSRLDRAAQDRLEAAARAACRPIDDKRGTIEFRTDVAAVLARRTA
                     LIALDRARAN"
     gene            complement(18626..20170)
                     /locus_tag="VFX87_04460"
     CDS             complement(18626..20170)
                     /locus_tag="VFX87_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994195.1"
                     /translation="MTLSRPASRLVLVAAILCAMLGLVGLSRAAAAPEGTLTIAMHFS
                     PVTRWLDPAEGESTITPFLLLYVLHDGLIKPMPGVGSGPSLAQSWAMSKDMLSAEFIL
                     RPAKFHNGEPVTAEDVKFSFERYRGGAAKVLKDAVKEIQTPAPNRVRFVFKQPWPDFG
                     AFFGTFVASAGWVVPKKYFESVGEEGFRKAPVGAGPYKFVSFNPGVELVVEAFDGYWR
                     KAPSIKRIVFRSLPDETTRAAALKSGEVDLAMLLSGPTAQDIKRTPGMRLAAPLLGIF
                     WLDFPDQWDPKSPWADRRVRLAASLALDRQALNDAETLGLSRPVGSIVPRDFEFALPF
                     EPHPHDPARAKKLLAEAGYPNGFDGGELTPFPPYNSMGETIQGWLQAAGIRTRVRSME
                     RGTFMTSWREKKLKGVVLTISGVSGNAATRLESFVTKNGAFAFGALPEVDDLFRRQAG
                     ELDRKKREAILHQIQRILHEQVTQAPVYHLGFPIGVGPRVDDIMATAIPGFYMSPYED
                     LKLRAR"
     gene            20247..21677
                     /locus_tag="VFX87_04465"
     CDS             20247..21677
                     /locus_tag="VFX87_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEX4994196.1"
                     /translation="MRCWLLIAALTVALTAPGPGTAAEPLSRDTGERESYVIPALEIA
                     GFLTGLNQVNRHFIDSKEYGSDGHSIWKNLREPLIIDEDPFSINQLGHPYQGNIYFGF
                     ARSAGLDFWRSLAYSLGGSLLWEIAGETTPPALNDHVNTAIGGAFIGEATFRMASLLL
                     EGGGEKPGFWRELGAAILSPPTGFNRLAFGDRFDKVFPSHRPEVFIRLRLGATLTNRV
                     DNDHLSRDVKRQEGAADYSITYGLPGKRDYQYKRPFDYFHFEFTAVPTAGEITDAIEN
                     VSVRGLIGGRTYELGDDFRGVWGVFGNYDYFSPQIFRVSSTGVSIGTVFQWWLTRQVA
                     LQGTALGGIGLGAAGTVGNRAEREYHYGLTPLSLLGFRLIFGERAMLEATGRQYYVAG
                     TSSNEESGVDTRNFSQDVIMRGSVGLTVRIYGPHALGIQYLVTSRNTGAPDLRDRHQS
                     VQTVTLSYNFLGHTRFGAVEWRPAAD"
     gene            complement(21704..22405)
                     /locus_tag="VFX87_04470"
     CDS             complement(21704..22405)
                     /locus_tag="VFX87_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994197.1"
                     /translation="MRRGSGLGAGALALALGLVAVADGAGTSTTFDFVTFDGIDYIRW
                     IDEPGRELTRADLGVEFGTVGCSIGEDSRTCSYGVDAGAAFMPAGTRMYAVRGYATEF
                     RLAAAWRDRLFLYQAWRNPRARVGGDLFDIAGRVRAIDVERRDGSRAPREATRPAPIA
                     SAHDVEALVGMILRGTMRRPTPHAASEPEYWLTIWLADGTTLGRPYYADTGELMGGLA
                     VPPEFRVLLERHLAG"
     gene            complement(22402..23790)
                     /locus_tag="VFX87_04475"
     CDS             complement(22402..23790)
                     /locus_tag="VFX87_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994198.1"
                     /translation="MTTPREALEQVWAVAGGEPAALGRVTLSGDDPLLPTDVRIGTAA
                     AAVIAATGLAVSELWRLRTGRGQAVAVDVRAAIAAFRSERYLRVNGGPAPDGRSPLFG
                     FYPTADGRWIQIHANMPHHHAGHVRFMGCEETRESMAAAVAKWKAQDLEDALNAAGLP
                     AGMVRPREEWLAHPHARAVAELPLLEVVPIGESAPEPFGGGDRPLSGVRALDLTRVIA
                     GPEGGRTLASHGADVLLVSSPHLPNLPSHVIDFGLGKRAATLDLRRAGDADRLRVLIA
                     QADVFSQSYRPGALARLGFAPEEVARLRPGIVYVTLSAYGHAGPWRERRGFETLIQSV
                     SGLAHEQGFGGGLGRPQHLPAQVVDHATGYLAALGALVALARRAREGGSYLVRVSLVQ
                     TGRWVDSLGRVAGRHVADQTAADVGDLLATCESPFGQLTHVTPTARLSETPPFWERPP
                     VPAGTHEAGWPT"
BASE COUNT         3576 a         8648 c         8381 g         3478 t
ORIGIN      
        1 aggtctccgc ctccgccgcg ccctggcccc gcgccccgcc gctgatcagc gccaccttgc
       61 cgtcgagctt gcccatggcc aggaattcta cgccggggtc aggtcttaca ttccgacatt
      121 tccggcgcta tgatcgccga ccatgggcaa gctcgagggc aaggtggcgt tgatcagcgg
      181 cggggcgcgg ggccagggcg cggcggaggc ggagaccttc acccgcgagg gcgcgaaggt
      241 cgtcttcggc gacatccggg acgatgaggg caagaaggtc gaggcggcga tccgggcggc
      301 cggcggcgac gccacctatc tccatctcga cgtcacgagc gaggcggact ggaaggccgc
      361 ggtgcagacc gcgatcgacc gctatggcaa gctcgacatc ctgatcaaca acgcggccat
      421 cgtcatcccg cgcgtgccga tcgaggagcg cacggtggcc gagtgggacc aggtcatggc
      481 ggtcaacgcc aagggcgtgt tcctcggcac caaatacgcg atcccggcca tgcgccgggc
      541 gggcggcggc tcgatcgtga acatctcctc gatcgcgggc atcggccagt cgctccacca
      601 ggagccggcc tacgccgcga gcaagggcgc gatccggatc ttcaccaagg tgaccgcgag
      661 ccagcacgcc aaggacaaga tccgctgcaa ctcggtgcac ccgggcccgg tggacaccga
      721 gatgttccac agcgccttcc gcgacaagga ggccatggcg cggcgcctgc agcggatccc
      781 gctcgggcgg atgggcacgg tggccgagat cgtgagcgcg gtgctctacc tggcctccga
      841 cgactcctcc tacatgaccg gcagcgaagt cctcatcgac gggggagcgc tggcgcagta
      901 gcaacgaagt ggggtcaggt cttgcattcc gacattcctt ggcgcgcagc gactcagcaa
      961 ccgggtgaaa tgtcgcaatg taagacctga ccccaaccct cgccgactag gtcgcggccc
     1021 gcgtgccccc gtaggcctcg ctcggcgcca ccttgctgtc ctcgaagatg agccgggcgc
     1081 gccggctcac caccgccgac cagttcgcga ccgcggtcgc ggaccactcg gcgtcgggcc
     1141 gcggctcgtc gtcgaagttg cccgaggtgt cgatgccgcg catcagcatc ccggcctgcc
     1201 gcacgcgggg cggcttcgcc caggtcggga acatctccac cagcaggagg aagcgccggt
     1261 cggggcccgc attcgtgccg gagccgtgca cgagcgtgtt gtcgaacagc accatctcgc
     1321 cgggctcgag cgcgaagtcc accgccttcg tctcgtcgaa gggatcggtg atgaattgcc
     1381 ccttggagag gatgctgtgg ggatcctcgc tctcgtcgtg cttcaggatc ccccacttgt
     1441 gggagccggg gatgccccgg aggcagccct gcgacacccc gcactccgag agggcgagcc
     1501 cccccagcac caccgggatc gccgccccgt aggccgaatc ctggtgccag cccgcaaagg
     1561 tctggccgtc ggccttcttc acccgccagg ccatgctcca gcagcggatg ttcgggccga
     1621 tcaggtcctc gaagacgtcg aggatgtggg ggtcctcggc gatctccagc acccacgggc
     1681 agagcaggtg cgacttggtc ttcatcttct tgatgtccgg gacgcgggct tcgagggcct
     1741 cgaaccgctc ccggtagtgg cgggcgcgct cggcgctcat cacccggacc ggggaaaggc
     1801 agccgtcccg ctcgaaggac cgaacctggg attccgtcag taccttgggc atccgaaacc
     1861 tccgcggggt caggtctgca agacctgacc ccaatcttga ctcccccggc gatggcccat
     1921 acagtagcgc aactcgacgc gaacctcacc cgcaaggagc cgcgaacatg agctactggc
     1981 gcacggcggt tgtcctcctc tcactcgtcc tggtcccgct caccgcggga gcggcccccg
     2041 cgggccgggt cgtgatcgcg cagggcgtcg accccacgac actcgacatg atgaaccagc
     2101 aggagcagcc ggcgtcgaac gtgggcgcgc agatgttcga gtcgctgatc gagcgcgacc
     2161 agaacctcaa gctggtgccc gcgctcgccg ccgagatgcc caagctggtg gcgccgacca
     2221 cctgggagtt caagctgcgc cggggcgtca agttccacaa cggcgaggac ttcaccgccg
     2281 actcggtcaa gttcagcatc gagcgcctgg tcaacccggc caacaagctg cggggctcct
     2341 cgtcgtttgc gccgatcgac cgggtcgaga tcgtgaatcc cttcacggtg cggatccaca
     2401 ccaagaagcc atggccggtg tttctcaatc acatcgcaca gcgccaggcc gcgatgtacc
     2461 cgcccaagga gtacgcgggc aaggacgcgg ccgcgatctc ccgggccccc atcggcaccg
     2521 ggccctacaa gttcgtccgc tggtccaagg acgaagagat cgtcatggag gccttcccga
     2581 gctactgggg cggcgcggcc aagatcaaga ccgtggtgtt caagccgatc cccgacgacg
     2641 cggtgcgggt ggccgcgctg cagaacgccg agatcgacgt ggcggtgaac atcccgcccc
     2701 acatggccgg gatcatcgag aagcacccga agatcttcct cagcaccgcg ccgtccatcc
     2761 ggacgatcca gctcatgatc tacacgcacc agatggacgc cgcccacaag cccaccggcc
     2821 cgtacgccct ccccaccgcg gacaagcgcg tccgccaggc gctgtcctac gccatcgacg
     2881 ccgacgagat catcaaggga gtgatggacg gcaaggcggt gcgggtggcg accatgctcc
     2941 cgagcatgca ctttggcttc gacccgtcgc tcaagccggt caagcaggat ctggcccgca
     3001 gcaagaagct gctgacggag gccggcttcc ccaacggcct cgagatcgtg ctgaacggcc
     3061 cgcaggggcg ctacgtgcgg gacaaggagg tcgcggaggc ggtcgcgggc cagctcacca
     3121 aggccgggat caagaccacg ctccggacct tcgagttcgt gaactacctg aacaacatgg
     3181 tgtacgtgca caaggcgggc ccggtctggc tcatcggctg gggccatccg acgatggacg
     3241 ccgaggccat ctacgtgccg ctgttcaagt cgcccaacat cttcgtgaac tggcacaacg
     3301 aggacttcaa cggcatggtc gagcaggcgc agtcggagat ggacgagaag aagcgcctcg
     3361 ccatgtacca ccggatcaac aagctgtgga tcgaggaggt ccccgcgatc ccgctctacc
     3421 agcagatcga cctctacggc gccaacaagc gtctgacctg gaaggcgcgc agcgacgaat
     3481 tgatccgcgc ctacgacatg gccatcaagt aacccgcagg gcgaggtgca gccgaaggcg
     3541 agccgagcgg atacaagaag agaggctagg ctgttagctc ctggccccag ggctccatgg
     3601 cgggccgcag cggggtgaac tcccgcacgc ggcccgccac gccgcccagg atgcggtcga
     3661 tcgtcgcccg ctcggccgcg gagagctcta gctcggcacc cgcgtcgttg gcgtccacct
     3721 cggccggcgt gcgggccccc accagcgcgg tgctgatcgc cgggttcgcc agcacccagg
     3781 cgagcgcgat ctggctcagc ggcaccccgc gcggctcggc cacctcgcgg cgcagccgct
     3841 ccaccacccc gacgttggtc ttgaggttat ccccacggaa gatcggctga ccgaacgcca
     3901 cgccgccggc gcgccagtcg cgcgcggggt cgaaggtcgt cgtcgcggtg aaggccccgg
     3961 tgagcaggcc gtggccgagc gagccgtagc ccatcacccc gatgccgtgg ctcacgcagt
     4021 acggaaacgt ctcctgctcc tggcgccggt cgagcatgtg gtagcccacc tgcgagacgt
     4081 ccacgcgccg ggtgcgcatg cactcggcca gcatggcacc ggtgaagttg gacacgccca
     4141 cgaagcgggc gcggccggaa gcgacgactt cctccagcgc gcgcatgctc tcgtcgaacg
     4201 gggtcttggc gtccggccag tgcacgagca gcacgtccac ccagtcggtg cgcaggcgct
     4261 tcaggctcgc gtcgatctcg gcgaggatgt tggtccggct cgcgtcgcgc agcgggcccg
     4321 gctggcccgg cggcggcgcc ttcacgccgc acttggtgac gaggatgacc tcctggcgct
     4381 gacccgcgag ggctcgggcg accacctctt cggaatgtcc ggcgccgtag gcgggcgcgg
     4441 tgtccacgca gttcaccccg cggtcgagcg cgcggtggat ggccttcacc gcctcgtcgt
     4501 cctcgatcgc gccgtagcga tcgccggcca tcggccagca gccgaagccc accacggaga
     4561 ccttcaaccc ggtgcggccg aatggacggt agcgcatggt cacccttcca atggcgcggc
     4621 ggcgccggct tcgagataga cgcgggcgac gcgcgggccg accgtcgtcg ccacgtggtg
     4681 aagcccgagg ccgtgtcgag acgcgacctc ggccgacgtg atctcgagat cgccctggcg
     4741 gccgatgatc acgacctcgt cgccgacggc ggcgtcggga accccggtca ggtcgatgcg
     4801 cgtgtgctcg agcgacggcg cgccgatcag cggcaccgcc cggccccgga cgaggacgcg
     4861 tccggcgtgg agccagcgca ggccgtcggc cgatcccatc ggcaccacgc cgaggcgcag
     4921 cggcgcggtc accgggaagg gggccgcctc ggcgaagcgc tcgcgcggcg ccagctcctt
     4981 gagcgcgatg acgcgcgtgg tgagggcgcg gagggccgag cgaaggggga ccggcggcgt
     5041 ctctcccgga aacgtgatgc cgtagagcat ccggccgggg tcgaccgcgt tgagatacgt
     5101 gtgcgggaag cgcagcacga acgggctcgc ggccaggagc cgcaccggaa cccggatccc
     5161 gcgcgcctcc agctcgtcca cgactccggt gaaccggccg agctgccagt cggcgtacgc
     5221 cggatcgccg cccacctcgg cgtggggatg cgcgcacagc cccccgaggc gcaggtggcg
     5281 cagctcgagc atcgcaacga tcagtttcac cgcttgttcg gcagggacgc cgaggcgctc
     5341 gaggccgacg tcgatcttca cgaacacctc gcacgggccg gtagcggccg ccgagtacac
     5401 ccgcgcggcc tcgagatcca cgagggtcgg aacgaggccg tgggcgagcg tctcggccgc
     5461 ggcgccgggc agggagttcg ggtagacgag gatcggcgtg gcgatccccc gccggcggag
     5521 ccggaccccc tcggtgagat cggccaccgc caggtagtcg gcgccgttcc ggacgaacac
     5581 cgctcccatc tcggcggccc cgaacccgta accgtccgcc ttgacgacgg cgaagatctt
     5641 ccgcgagggg ccgacgagcc gccggaccgc gcggagattg tccgccgccg cgtcgagatc
     5701 gatctcgagc gtggtgggac ggagcggacc gctgggcatt gcgcctccgc tacgcggtgg
     5761 ccgccttcag atcgtcgagc tgcttgcgga cgcccgcgac catggacgcc aggtcggagc
     5821 agagcatgac caggtcgaag ccgcgcttca ccgaccccgc cgcgaacgcc gcgctcgcgc
     5881 agtgcatcgc cgccttcttg ccgtgcttgt gggccgcgtc gcgaatccgg tcgcaagtgg
     5941 cgatgtgcac cgggtccggg ttgtcgcccc gcggggcgag cccgagcgcg aacgagagat
     6001 cggcggggcc gatgtagatg caatcgagcc ccggggtggc gcagatcgcg tccaggttgg
     6061 cgaggccctc cttggtctcg atcatcgcca tgaccacgat ctcgtcgttg gctcgggcga
     6121 cgtagtcggc gccgccgtag tgcaccgcgc gcaccggccc cgaggagcgc atgcccaccg
     6181 gaggatagcg gcacgcggcc accgccttcg cggcgtcctc ggccgtgttg atgagcggca
     6241 cgaggatggc gtaggcgccc aggtccagcg ccttcatgat cgcggcaggg tcgttcgagg
     6301 ccacccgcac caccggcacc gtgtcggtct gggagaccgc ctgcagcatg ggccagaggt
     6361 tggccatgtc ggtgaggccg tgctgcatgt cgatgacgag ggcgtcgaag ccctgccggg
     6421 ccatcacttc cgcggtgaac ccgttggaca ccgagagcca gcccatcgtc acgcacttgt
     6481 tctcgcgcca catctgcttg atcttgttcg gcctcatcgt cacacctcgt cggtaaaaga
     6541 atgttcggcg ctgaagcgta tcacagcctt tggtggtata acacgcgcca tggatctcga
     6601 gctcgcggac aaggtggcga tcgtcggcgg cgccagcaag ggactcgggc gggcctgcgc
     6661 ggaggtgctg gcccaggaag gctgccgcgt ggcgatctgc agccggtcgc gcgacgatct
     6721 ggagaaggcg gcgaaggaca tccacggcgc gaccggccgc gaggtgctcg tcttcgcggg
     6781 cgacctggac cggcccgaga ccgtccgcgg cctcgtggcc gccaccgtgg agcgcttcgg
     6841 acggctcgac atcctggtca acaactcggg cgggccgccg ctggcccagg cccagaccgc
     6901 cagcgaggag cagtgggcca ccgcggtgga acggtcgctc ttcttcttcg cccggatgtg
     6961 ccgcgaggcg ctgccccatc tcaagcggca ggccgccggc cggatcatca acatcctggc
     7021 cagcaccgtc taccagccga tccccaacct ggtgctctcc ggagtcaccc gcatgggcgt
     7081 ggtggccttt gccaagaccc tggccgacga ggtcgggcgc gacggggtcc tggtcaacaa
     7141 cgtgtgtccg ggctcgatca tgagcgagcg gatgctctcg aacgtgaccg cgcgggccac
     7201 gcagctcggc atcacgctgg aggaagcgct ggcccagcgc gcggcggaga ccgcggtggg
     7261 gcggctcggc gagccccgag agctggccca cctggtggcg ttcctcgcct ccggcaagtc
     7321 cagctacatc accggcacca ccattctcgt cgacggaggt ctcgtgcgct cggtgctctg
     7381 agccgtccgg accgggaggc gaatgacgcc aatgtcccac acccgccgtg acctgctgaa
     7441 aggagccgcc gccctcgccg ccgccgcaac cgtcagcgca ggagaagcca cgatgtcccg
     7501 ccaagctcac gctcaaggaa gcgccggtcc ggcccgcgcg cgaatcgacg gcgtgctccg
     7561 ccaggccgtc gacgccaagg aggtgccggg cgtggtcgcg atggcggcca ccgaccaggg
     7621 cgtgctctac gaaggcgcct tcggcccacg cgcgctggag ccgggcgcgc ccgcgatgac
     7681 gatggacacc gtcttccgca tcgcctccat gaccaaggtg atcacctcgg tcgcggcgat
     7741 gcagctcgtc gagcagggca agctcaagct ggaagagccg gtgcccaaca tcgatccctc
     7801 gctgggcgcg ccccaggtgc tcgacggctt cgacgcggcc ggcgcgccga agctcggcgc
     7861 cgccaatcgg ccgatcaccc tgcgccacct gctcacccac accgcgggct tcagctacga
     7921 catctgggac gcgagcaccg ggcgctacgt caaggcgtcc ggcatgcccg cgcgggccac
     7981 cgccaaggtg gcctccatcc gcaccccgct gatcttcgat ccgggcgatc gctgggagta
     8041 cggcgtcaac accgactggg tcggccgcct cgtcgaggcc gccagtgggc agccgctcga
     8101 cgcgtacttc cgcgacaaga tcttcgcgcc gctcggcatg aaggacagcg gctacgtgac
     8161 ctcgaccgag cagcgcgcgc gccaggcgcg gctgcacctg cgccaggccg acgcggcgct
     8221 cgccccgcag ccgctcgagg cggtgccggt ggccggcgag ttctggtcgg ggggcggccc
     8281 gctctactcg acgggacgcg actacatcga gttcctccag atgctgctgc acggcgggag
     8341 ctggaagggc gcccgtgtgc tgaagccgga gacggtggcc ctgatgcagc agaaccacac
     8401 cggcagcatc ccggccggga tcctgaagac ccagatgccc gccctgtcca acgacgtcga
     8461 tctcttcccc ggggcccaga tccggtgggg gctcggctac atgctgaacc ttcagcccgg
     8521 gccgaacggc cggagcgcgg gcacggtgag ctggggcggg atcttcaaca cctactactg
     8581 gctcgacccc gcgaagcgcg tgaccggcct gatcatgacg cagatcctgc cgttcgccga
     8641 ccaccgcgcg ctcaagctct acgggcaatt cgagagcgcc gtgtacgagg ccctgccgaa
     8701 agcctgacct aggcgatcga gtccgggcgc accgcgtgtc cggtgcgtcc ggactcgtag
     8761 atcgccagcg acacctggag catgtgccgg gccgcctgga cgtcctgggc cggctggcgc
     8821 ccgtcgagca ccccgtccac gaagtccgcg gcggcgccgt cgaagccgag acgccagtcc
     8881 atcggcacct ggtagctgac ggtctcggtg ccgcggatca cctgtaccgg cggcaggtcg
     8941 agcatctccc cgctgcagcg ggtcacccag atgatgccgc gactgccgtg gatctcgaag
     9001 aactcgtcca cccggacgta ccgcccgcgg atggccatct cgggcgcgta ggtgtagtcc
     9061 atgatcccga ggcagtcccg gccctcgaag cgccaggtgg ccacgctcgg cgtctccatg
     9121 atgaagtccg ggccgcgcgt gacgatcgcg gagacctcgc cgatctcgcc gatccagtag
     9181 gcggcggtgc cgtacttgtg cacgccgtcg tcgaagagca ccccgcccgg gttccgcgcg
     9241 gggtcgcggc gccagaccat cgcgtccggc tccatgtcga gcgtgatgcc ctcgacgcgc
     9301 tgggcgcgcg tggtgtggat gcgcaccagc gagggctcgc cgatcgcgcc cgcgtcgagc
     9361 agcgccttgg ccttcacgat gggcgggtag tagaggaagt tctcggtgac ccggaaggtg
     9421 gtgcgcgccc gggccaccgc cgccgcgatg cgatcggcct cggccaccgt gatcgccagc
     9481 ggcttctgac acgatacgtg cttgcccgcg tcgagcgcgg ccacgatctg gtcggcgtgc
     9541 atccacgtgg gcgtgagcag ctccaccgcg tccaccgcgg ggtcgtccag cacctgcgcg
     9601 aagtcggaac acagacgcgg cgtgatcccc cactcggtgg cccgccgctt ggcccgctcc
     9661 ggccgcgtgt cgcagagcgc gaccacgtcg cagcgcgggt gctggagata cccgggcgcg
     9721 ttgagccgcg agatgttgcc gcatccgacg atggccagcc gcaggcggtc catggagcgg
     9781 ctcgagtcag gcagggagct tgtcgccgtc gggcggggcc gccacctcga aggcgttgac
     9841 caccccgcac agcatcgcga agtagctcgc ggccgccgtc atctcgacga gccaccgggg
     9901 gtcgtgccgc ttcgcgagcg cgtcgaaggc cgcctggtcc accttgttcg tgcgcatgag
     9961 ctggcgcacg tagccgatga tctcgcgctc gtcggcgggc aggcccgacg ggtcgccctt
    10021 ggcgcggagc acgtcgatga tcgcctcggc cactccattg cgccgcgcct gtccgacctg
    10081 ggccgaccag acgtaggcgg cctcgcgctc gcgcaccgcg gccaggatgg ccaccgagcg
    10141 gagcttgccc tcgaccgcgc tctcatcgcg gaagaacgtg accaggggca gcaggcgctc
    10201 ggcgagctgc gggctgtgga gcagcatgct gaacggcccg cgcacgccgc cgaagacctt
    10261 gacgacctgg tcgacgactc cgtggtgggc ggcggggaca tccgacttgc cggtgatggg
    10321 gacgatgcgt ggcatggagc gctcctctct cgggtgatca gccgttggtc agcccgcgca
    10381 gcacgtcgaa gtactccggg aacgtcttgg acacgcaggc cgggttgacg atgcggatgc
    10441 ccggcgcgcg caggccggtc accgcgaagc tcatcgcgat gcggtggtcg tcgtaggtct
    10501 gcacgtcggt ggggcggggc gtgccgggat ggatggtcac cgaatcccac gcgctctcca
    10561 cctgcagccc catgcgccgc agctcggtgg cggcggccac cggccggtcg ctctcctcga
    10621 agtgggtctg ggcgatgccg cgcaccgtca ccggcccgtc cgcgaagggc gcgatggcca
    10681 tcagcgtggt cgccacgtcg cccatccggg tgaagtcggc gtccaccgcg agcagccgcg
    10741 ccgggccccg caccgtgatc gaatcgtcct ccaccgccac ctcgcagccc attcgggcga
    10801 gcacgtccac caggccgaga tcgccctggg cggacaggcg gccgagccgc ggcaccacca
    10861 cgcggctgcc ggtcaccgcg gcggccgcga agaagtagga cgcggcagag gcgtcgggct
    10921 ccaccgcgta ggcccgcgcc tcgtagcgct gcccggccgg gacgcggaag cgccggtcgc
    10981 cctcccgctc ggcgcgcacc ccgaaggcct ccatggtgga gagcgtcatc gtgatgtagg
    11041 gcgccgagac cagctcgccc tccacctcga tctccacgtc gtggcgggcg tagggcgcgg
    11101 cgagcaggat cgcgctgaag tattggctcg agatgtcccc gcgcatgcgg gcgcggccac
    11161 ccgcgatgcc cttggcccgc accaccaccg gcgggcagcc ggtgccctcg cggctctccg
    11221 cgtccacccc gagcgcgcgg aggccgtcga gcagcggctg gatcgggcgc ttgcgcatgg
    11281 ccggggagcc gtccaccacg aaggtcccct gcccgagggc cacggcggcg gtcaggaagc
    11341 gcgcggcggt gcccgcgttg ccgatgtcga gcgtggcctc cgcggcgggg aagcggccgt
    11401 ccacgccttc caccgcgaag gcgcgggccg cctcgtcggc cgtcacgcgc accccgagcg
    11461 cctcgagcgc ccggtgcatg tagcgcgtgt cgtccgacca gagggcctgg gtgatctgcg
    11521 agcgcccgcg ggccagcgcc gcgaccagga gcgcgcggtt gctgtagctc ttcgacccgg
    11581 gcagcgccac ctccgcgtcg aacggctgcg tgatcggctg gacgggccgg acgggggctg
    11641 gtgtctccgc catgattggc gcatcatacc cccggtccgt gatcgaggct cactcaaacc
    11701 ggtaggagac cgctgatggc catcacgatc cgacaggtcg gaccctgctt tgccggcgag
    11761 gtaaatggac tcgacctcgc ccggccgctc tccccggaag acgcggtcgc catccacgcc
    11821 ggcatggacc gctacgcggt gctcgtgttc cacggccggc ccgtcactcc cgaccagcac
    11881 atggccttca ccctggcgct cggcgcgatc gagaacgccg tcggcaccgg gctgcgcgag
    11941 aactaccgcc tgcccaccgt gttcgccgac gtgtccaatc tcgacaagga cgagcgggta
    12001 ttcgcgcggg acaaccggac gcggctcttc gggctcggca accgcctctg gcactccgac
    12061 agctcgttca agccgatccc cgcgaagtac tccctgctgc acgcctaccg catcccgtcc
    12121 cggggcggca acaccgagtt cgcggacatg cgcgcggcct gggacgccct cgacgcggag
    12181 acccaggccg cgtgcgaggg gctgatctgc gagcactcgc agctctactc gcgacagatc
    12241 ctgggcttca ccgatttcac cgacgaggag cgcgagcgct tcgccccggt gcgccaggtg
    12301 ctggtgcgca cccacccggt gaccgggcgc aagtcgctgt atctctcgtc acacgccggc
    12361 ggcatcatcg gctggccggt gccggaggcg cgcgccttcc tgcgcgacct ggtcgagcac
    12421 gccacccagc ggcagttcgt ctactcgcac cggtggcagg tcggcgatct cgtgatgtgg
    12481 gacaaccggc agaccatgca ccgcgcgcgg cccttccccc agcacgagcc gcgcgacgtg
    12541 cgccgcacca ccctggcggg cgaccggccg acggtggcac aggtcagctg acgcgccagc
    12601 tcttgtccac gtagcggcgg ccgtccaccg tcgagatctg cagcgcggcg cggtaggcac
    12661 cggccgccgc cgtgtggacg cgcgtctcca ccgcgacctg ctccgggtcg ggcccgccct
    12721 cgagcgcctc ggtgaccacg cggccgtcga ggccggcctc gccgtcgagg cccagcaggg
    12781 cgagcgcggt cggcgtcacg tccacgttgc cggccggcgc gcgtaccgtc acgcccttct
    12841 tgactcccgc gccccaggcc aggaaggtat tgcgcacgtt ccacgggctc atgctgccgt
    12901 ggtcgctcgc gtagggggcg ccgcccgcgc tcacgttcgc ccagtcggtg ccgggcaccc
    12961 cgaaggcgtt gcgccgcgac gtccacggga acgtgaagag cagatcgggg ctgcgatcgc
    13021 ggttggccac gttgatcagc tcgaggggga aggcgccctc caccggaccg gccgcgaaga
    13081 ccacgccgcc ccagtcctgg gcctggatga agcgggccag ccgcgcgatc cgctccgcgt
    13141 cgtgcccctc gacgtggatc gcgacggcct ggccgctgct ggcgaggatg acgtcggtgg
    13201 aatcgggcgc cgccttcagc cgggccgcga cgagggcgcc cgccacgtcc accccggcgg
    13261 tattggtggt gaacccgtgg tccgagacca ccagcacgct ggtcgccggg gtgaggccga
    13321 gggcgtcgag cgtcgccagt acccgggcga tctcgcgatc gtcgttgcgg atcgcctcgc
    13381 gcgccgacgg cgagcccacg ccgacgtggt gctggctgtg gtccggctcg gtgagccagt
    13441 tgatgaccac cgcgggcccg agctccggta gcacgtactc gcgcagcacc cgctgggtcc
    13501 agttgacctg agcgtcgaag cgcccacccc cgggcgcctt caccggtgcc ggcccgagcc
    13561 ggctgaggat gctctcgctg accccggcgg ggtaggccac cgtgtggccc gggtcgaggt
    13621 agccgttgac gagcgcgccg acgcccgccg gcgcgcgggg attgagcagg aacgcgctgc
    13681 ccgtcgaccc cgagctgacg ccggccagga cgagcccgcg ggcgtgcagg cgctcggcga
    13741 gcgtcggaac ctcgagcacg cggccatcgc tggcccgatc gagcgcgagg aagttccggt
    13801 ggttgccggt gtcgaacgcg cgccgctcgt ccaccgcggg cacgaacatc tgattgccca
    13861 ccagcccgtt ggtgcccggc tgtgtcccgg tggcgatcgc ggccgcattg acgcgggtca
    13921 cggtggggaa gaccgcgtgg ccgttcacca gatccacgcc ctcggcgcgc agccggaaga
    13981 gggtgggcgt gtcctcggcg gtgatggagt cggggcggag cccgtccacc acgaagacga
    14041 ggacgagggg ccgcgcggcc ggcactaggc ggggtcgatt ccgacgatct cgttgaaccg
    14101 gccgaagccg atgtagagcg tggtgtgcgc caccagctcg atcacctcgg cctcggagaa
    14161 ctgggcgcgc acctcggccc agagccggtc atccaccttc tggtgatcca ccgcgagctt
    14221 ctcggcgaag cggaccgcgg cccgctcgcg cggggtgagg agcgtggagg tctcgtcgtc
    14281 gatcgccgcg atcttctctt cggtcgcccc cgcatgccgg gccccggcat agcgcgcctt
    14341 gagtcagtag cggcaggcgt tgagcccggc gatcttgatc cgcaccagct cgcggatgac
    14401 cgggccgatg gtcccctcgc gcacgagcgc gccgcggaag gcggccagcg ccttcaccac
    14461 cgcggggttc cgggcgtagg tgcgggtgct cgcggaggtg ccgatctgcg cctccgactc
    14521 ccggcacatg tgctggatct cggggtcgag gtcgtgcatc gggagcggct gcatgcgcgc
    14581 cattgtcgcc tcctaagatg gggtcaggtc ttacattccg acatttttgg acccgcgacc
    14641 gatagagttg cgcgaggaaa tgtcggaatg taagacctga ccccggctcc tccggcgatc
    14701 gccgggagaa ggaagacttc gctgttggca ccgatcggct cgagcagggc gtcctggaag
    14761 atctggccgt cgatggccac cgcgacgccg ttctcgagat ggggcgcgag cgccggatgc
    14821 agctcgccga gctgccggaa gagctgctgc acggacgccg ccgagagatc gaactcggcg
    14881 acgcctccgg tcacctgcgc gaggttgccg accagcacca ctcgcgccat gcggtcgcgg
    14941 ctcaggcctt gctgaggccc ttctccttga gcgccttcag caccttcggc ggcgacatcg
    15001 gcagctcggt gaagcggatt ccggtggcgt tctccatggc gttggcgatg gccccgagcg
    15061 gcgggacgat cggcgtttct cccacccccc gcacgccgta gggatgcttc gggttggcga
    15121 cctcgaccag gatcgcgtcg atcatcggca cgtccgaagc caccggcatg cggtaatcga
    15181 ggaagcccgc gttctgcagc ttgccgtcag cgccgtagat gtactcctcg ttgagcgccc
    15241 agccgatgcc ctgcaccgcg ccgccctggt actgcccctc gacgtaggcc ggatgcagcg
    15301 cccggcccgc gtcctgggcc accgtgtagc gcaggatcgt cgcgcgcccg gtctccggat
    15361 ccacctcgag gtccaccagg tgcgtgccga agctcgggcc cgcgccctgg gcgttgatgc
    15421 ggccgacgcc gccgatgggc ccgccggtct tgccggcggt cttggcgaag tcctcgacgg
    15481 tcatcggctt caccttctcg gcgagcgcgc cgagcggcct caccgcgccg tccttccact
    15541 cgacctggtc cggcttggcc tcccacagct tgcaggcccg cgcgcagaga tcggccttga
    15601 tctgcttggc cgcctccacg atggccatcg agcccgagaa ggtcgagcgg ctgccgccgg
    15661 tgaggaagtt gaagccgagc gagccggtgt cgccgatgat cggccgcacc ttgtcgtagg
    15721 aaacccctag ctcgtccgcg accaccgagg ccagcgaggc gcgcagcccg ccgatgtccg
    15781 gcgtgccggc catcagcgag accgtgccgt cctcgttgac ggtgagcgag acggtggtct
    15841 cgccgccgat attgaaccag aagccggagg ccaccccgcg gccctgccac ttccggagcg
    15901 gggtgttcca gtgctcgctc ttcttgatcg cgttcagcgt ctcctcgagc ccgatcgggc
    15961 cgaacttggg gccgtaggcc gccttcgtgc cctcgcgcgc cgcgttcatc atccgcagct
    16021 cgatcgggtc gaggcggagc ttcttggcga tctcgtccac cacgctctcg accgcgtact
    16081 ccgagatcgg cgcgccgggg gcccggtacg cggccacctt cggccggttg gacaccacgt
    16141 cgtagccgat cacgtgcacg ttctcgatgt cgtagggcgc gtaggcgcac atgacgccgg
    16201 gctgcaccgg cgagccctgg aaggcgcccg cctggtactt gagctctgcg gcgccggcca
    16261 cgaacttgcc gtccttggtg gcgcccatct tgacccgcac ggtggcgccg gaggtcggcc
    16321 cgctggcctt gaacacctcc tcgcgcgaca tgaccatctt caccgggcgc ttggccttcc
    16381 gcgacagcgc gatggcgagc ggctcgaggt agaccacggt cttgccgccg aagccgccgc
    16441 cgatctcggt ggcggtgacg cgaaccttgc ccatgtcgag gccgagcagc cgcgcgcagt
    16501 gggcgcgcac gatgtagtgg ccctgggtgg tggtccagag ctcggtggcg ccatcctcgg
    16561 agacggtggc cagcgcggcg tgcggctcga tgtagccctg atgcaccggt gccgtcttga
    16621 actcccgttc gatgatgacg tcggccttct tgaagccggc ctcgacgtcg cccagcttga
    16681 tctcgacccg cttggcgatg ttcgagggct tgtccggcct gggctcgacg ccggccgtga
    16741 agagatcgtc gtgcagcagc ggggcgccgg gccgcatggc ctcgaccacg tcgatgacgt
    16801 gggggaggat ttcgtatttg acctcgatca gcttgagcgc gcgccgggcg atcgcggtag
    16861 tggtcgcggc gaccgcggcc accgggtggc cttcgtagag cgccttctcg cgcgccatca
    16921 cgttccggac cacgtccttg tagttcatca tcatctcgcc ggccgggatg aactccgacg
    16981 gctggtccgc gaagtcgtcc cgggtgatga ccgccttcac gccgggcagt gcctcggcct
    17041 tggacgtatc gatcgagacg atgcgcgcgt gcgggtgcgg gctgcgcagc acgcggccga
    17101 tgagctggcc gggcaggttg aagtccgcgc cgaaccgggc gcgcccggtg accttgtcca
    17161 cgccgtccgg gcggatcgtg cgggtgccga cgtagtgctg cccgttcgtt ccgttcgaag
    17221 tggaagctct gttcgtctcg cccatggtct taaccctttc tgagaatcgc ggccgtgtcc
    17281 atcaccgcgg tcacgatctt gtcgtacccg gtgcaccggc agaggttgcc ggccagccag
    17341 aaccgcacct cgtgctcgct cgggttcggg ttcttctcga gcagggcctt cgaggccacc
    17401 aggaagcccg gcgtgcagat cccgcactgg agcgcggccg cctccaggaa gcgccgctgc
    17461 agcggatgga gcgactcgcc cttggcgagg ccctcgatgg tctcgatctt ccggccgccc
    17521 acctcgcaac cgagcacgag gcaggagcag accagccgcc cgtccagcat cacgctgcag
    17581 gcgccgcagt cgccggagga gcagccttcc ttgctgccgg tcaggtcgag cgtatcccgg
    17641 agcacgtcga gcagcgtgtc ctccgtctcg cacaggaact cgaccgggtc accgttcacg
    17701 gttgcttcga tgtgatgctt ggccatcagt tcgccctcgc tcggtccagc gcgatcaggg
    17761 cggtccgccg ggccagcacg gccgccacgt cggtgcggaa ttcgatggtg cctcgcttgt
    17821 cgtcgatcgg ccggcaggcc gcccgcgccg ccgcctcgag ccggtcctgc gccgctcggt
    17881 cgagccggct cccgatgatg gcctgggctg cctcggcaac gagcagcacc cgcgcggcca
    17941 ccgcgcccag gctcacccgg gccgcgatga tggtcccgcc ggcatcgagc gtcaagctta
    18001 ccccggcccc caccaccgcg atatccattt cggtgcgcgg gatgaagcgg agataggcat
    18061 cgctcgagcg cgcgggccgg ggcggcagca agaacgacac gacgatctcg cccttggtca
    18121 gcgccagctt gcggggcccg agcatgacgt cctcgaccgg caggtcccgc cggcccttgg
    18181 gcccggccag ggtggccagc gcgcccgccg cgatcagcgc gggcacgctg tcggcggccg
    18241 gggagccgtt gcagaggttg ccgcccagcg tggcgcggcc ctgcacctgg gtggagccga
    18301 tgaggttggc ggcctccacc acgcccggcc agaccttctt gagccgcgcg tgctccttca
    18361 gctccgcccc cgtcaccgcg gccccgatgc gccagccgcc cttctcctcg gtgaccgtcc
    18421 gggtctccgg gatcttcttg atgtccacga tcagctcggg gtccaccatg tcggcgcgca
    18481 tctgcaccag gaggtcggtc cccccggcca gcacccgcgc ctcgcccttg gctcccgcga
    18541 gcagggcgac ggccccctcc agtgattccg gtgcttcgta tcgcatcgtc tctggtctcc
    18601 tgtatgctcc ggccggcatc atagctcagc gtgcccggag cttgaggtcc tcgtagggtg
    18661 acatgtagaa gcccgggatc gcggtggcca tgatgtcgtc cacccgcggg cccacgccga
    18721 tcggaaagcc caggtgatag accggcgcct gggtgacctg ctcgtgcagg atgcgctgga
    18781 tctggtgcag gatcgcctcg cgcttcttcc ggtcgagctc gccggcctgg cggcggaaga
    18841 ggtcgtccac ttctggtaga gccccgaagg cgaaggcgcc gttcttcgtc acgaacgact
    18901 cgagccgcgt cgccgcgttg cccgagaccc cgctgatggt cagcaccacg cccttgagct
    18961 tcttctcgcg ccacgacgtc atgaaggtgc cgcgctccat gctgcgcacg cgcgtgcgga
    19021 tgcccgccgc ctgcagccac ccttggatgg tctcgcccat ggagttgtag ggcgggaacg
    19081 gggtcagctc gccgccgtcg aagccgttcg ggtagcccgc ctcggccaga agcttcttcg
    19141 cccgcgccgg atcgtgggga tgcggctcga agggcagcgc gaactcgaag tcacgcggga
    19201 cgatgctgcc gacgggacgc gagaggccga gggtctccgc gtcgttcagc gcctgccggt
    19261 ccagcgcgag gctggcggcc agccgcacgc gccggtcggc ccagggggac ttgggatccc
    19321 actgatccgg gaagtcgagc cagaagatgc cgagcagcgg cgcggccagg cgcatacccg
    19381 gcgtgcgctt gatgtcctgc gcggtcggcc cgctcagcag catggccagg tccacctcgc
    19441 cgctcttgag cgcggcggca cgggtggtct cgtcgggcag gctgcggaag acgatcctct
    19501 tgatggaggg cgccttgcgc cagtagccgt cgaaggcctc gacgaccagc tccacgcccg
    19561 gattgaagct cacgaacttg tacgggcccg cgcccaccgg cgccttgcgg aatccctcct
    19621 cgcccacgct ctcgaagtac ttcttcggga ccacccaccc cgcactggcc acgaacgtgc
    19681 cgaagaaggc cccgaagtcg ggccacggct gcttgaacac gaaccgcacc cggttcggcg
    19741 cgggggtctg gatctccttg accgcgtcct tcagcacctt ggcggcgccg ccgcggtagc
    19801 gctcgaagga gaacttcacg tcctccgcgg tgaccggctc gccgttgtgg aacttcgcgg
    19861 gccgcaggat gaactcggcc gagagcatgt ccttcgacat ggcccacgac tgcgcgaggc
    19921 tcggcccgga gcccacgccg ggcatcggct tgatcagccc atcgtggagc acgtagagca
    19981 agagaaaggg cgtgatcgtg ctctcgccct cggcggggtc gagccaccgg gtgaccgggc
    20041 tgaagtgcat cgcgatggtg agcgtgccct ccggcgccgc ggccgcgcgg gatagcccca
    20101 ccaggcccag catggcgcag aggatcgccg ccaccaggac aaggcgggag gccggtcgtg
    20161 aaagggtcat ggatcgagac tcttgaacgt tcgcgccccg ttgtcaaggg catggggcac
    20221 aatgagccct tggacaccga cccgccatgc gctgctggct cctgatcgcg gccctcaccg
    20281 tggcattgac ggccccgggc ccgggaacgg ccgccgagcc cctctcgcgg gacaccggtg
    20341 agcgcgagag ctacgtcatt cccgcgctcg agatcgccgg gttcctcacc ggcctcaacc
    20401 aggtcaaccg gcacttcatc gattcgaagg agtacggcag cgacgggcac tcgatctgga
    20461 agaacctccg ggagccgctg atcatcgacg aggacccgtt cagcatcaac cagctcggcc
    20521 atccctacca gggcaacatc tacttcggct tcgcgcggtc cgccggcctc gacttctggc
    20581 ggtcgctggc ctacagcctg ggcggcagcc tgctctggga gatcgccggg gagaccacgc
    20641 cgcccgccct caacgaccac gtcaataccg cgatcggcgg ggccttcatc ggcgaggcga
    20701 ccttccggat ggccagcctg ctgctggagg gcggaggcga gaagccgggg ttctggcggg
    20761 agctgggtgc cgcgatcctc tccccgccca ccgggttcaa ccggctcgct ttcggcgacc
    20821 ggttcgacaa ggtcttcccg agccaccgcc cggaggtctt catccgtctc cggctcggcg
    20881 ccacgctcac caacagggtg gacaacgacc acctcagccg agacgtgaag cggcaggagg
    20941 gcgccgcgga ctactcgatc acctacggcc tcccgggcaa gcgggactat cagtacaagc
    21001 gcccgttcga ctacttccac ttcgagttca ccgcggtccc caccgcgggc gagatcacgg
    21061 atgccatcga gaacgtgtcg gtccgcgggc tcatcggggg gcggacctac gagctgggcg
    21121 acgacttccg cggcgtgtgg ggcgtcttcg gcaactacga ctacttttcg ccgcagatct
    21181 tccgggtgtc gagcaccggg gtgtcgatcg gcaccgtctt ccagtggtgg ctcacgcgcc
    21241 aggtcgcgct gcagggcacc gcgctcggcg ggatcggcct cggcgcggcg ggcaccgtcg
    21301 gcaaccgggc cgagcgcgag taccactatg ggctgacgcc gctgagcctc ctcggcttcc
    21361 gcctcatctt cggtgagcgg gcgatgctgg aggcgacggg ccgccagtac tacgtggccg
    21421 ggacgagctc gaacgaggaa tccggggtgg acacccgcaa cttcagccag gacgtgatca
    21481 tgcgcggcag cgtggggctc acggtgcgca tctacgggcc gcacgccctc ggcatccagt
    21541 atctggtcac gagccggaat acgggcgccc ccgacctccg cgaccggcac cagtcggtgc
    21601 agaccgtgac gctgtcctac aacttcctcg gccacacgcg cttcggcgcc gtggagtggc
    21661 gcccggccgc cgactgaggc ggcccgcgtc acccggcccc gcctcacccg gcgagatggc
    21721 gctcgaggag cacgcggaac tccggaggaa ccgcgaggcc tcccatcagc tcgccggtgt
    21781 cggcgtagta gggccggccg agcgtggtgc cgtcggcgag ccagatcgtg agccagtact
    21841 ccggctcgct ggccgcgtgg ggtgtcggcc ggcgcatggt tccgcggaga atcatcccga
    21901 cgagcgcctc gacgtcgtgg gccgaggcga tcggcgcggg gcgcgtcgcc tccctcggcg
    21961 cgcgcgaccc gtcgcggcgc tccacgtcga tcgcgcgcac ccggcccgcg atgtcgaaga
    22021 ggtcgccacc aaccctggcc cgggggttgc gccaggcctg gtagaggaag agccggtccc
    22081 gccaggccgc ggcgagccgg aactcggtcg cgtacccccg caccgcgtac atccgggtgc
    22141 cggccggcat gaaggcggcc ccggcgtcga ccccgtacga gcacgtccgg gaatcctcgc
    22201 cgatggagca tcccacggtc ccgaactcca cgccgaggtc ggcccgcgtc agctcgcggc
    22261 cgggctcgtc gatccagcgg atgtagtcga tgccgtcgaa cgtgacgaag tcgaaggtgg
    22321 tcgacgtccc ggccccgtcg gcgaccgcga ccagcccgag cgcgagcgcc agcgcgcccg
    22381 cgccgaggcc gctcccgcgc ctcacgtggg ccagcccgcc tcgtgcgtgc ccgccgggac
    22441 cggcgggcgc tcccagaatg gcggcgtctc tgacaggcgc gcggtgggcg tcacgtgggt
    22501 gagctgcccg aacgggctct cgcacgtcgc gagcagatcg ccgacgtcgg ccgcggtctg
    22561 gtcggccacg tggcggccgg ccactcgccc gagcgagtcc acccagcgcc cggtctgcac
    22621 gagcgagacg cgcacgaggt agctcccgcc ctcgcgggcg cggcgcgcga gggccacgag
    22681 cgcgccgagc gcggccaggt acccggtggc gtgatccacg acctgcgcgg gcagatgctg
    22741 cgggcggccc agcccgccgc cgaatccctg ctcgtgggcg aggccgctca ccgactggat
    22801 cagcgtctcg aagccgcgcc gctcgcgcca gggacccgcg tggccgtagg cggacagcgt
    22861 gacgtagacg atcccgggac gaagccgcgc gacctcctcg ggagcgaagc cgagccgggc
    22921 gagcgcgccg gggcggtacg actgggagaa gacgtcggcc tgcgcgatca gcacccgcag
    22981 ccggtcggca tccccggccc ggcggaggtc gagcgtggcg gcccgcttcc cgagcccgaa
    23041 gtcgatgacg tgggagggca ggttcggcag gtgcgggctg ctcacgagca gcacgtccgc
    23101 cccgtgggag gcgagcgtcc gcccgccctc ggggcccgcg atgacacgcg tgaggtcgag
    23161 ggcgcgcaca ccggagagcg gccgatcccc gccgccgaag ggctccggcg ccgactcgcc
    23221 gatggggacg acctcgagca gcggcaactc ggccaccgcg cgcgcgtgcg gatgcgcgag
    23281 ccattcctca cgcgggcgga ccatgcccgc gggcagcccg gccgcgttca gcgcgtcctc
    23341 gaggtcctgc gccttccact tggcgaccgc ggcggccatc gactcccgcg tctcctcgca
    23401 gcccatgaag cgaacgtggc ccgcgtggtg atgcggcatg ttcgcgtgga tctggatcca
    23461 gcgcccgtcg gcggtgggat agaagccgaa gagcgggctg cggccgtcgg gcgcggggcc
    23521 gccgttcacg cgcaggtacc gctcgctgcg gaaggccgcg atggccgcgc gcacgtccac
    23581 cgcgaccgct tgcccgcgcc cggtgcgaag gcgccagagc tcgctcaccg ccagcccggt
    23641 ggccgcgatc accgccgcgg cggcggtgcc gatccggacg tcggtcggca gcagcggatc
    23701 gtcgccgctc agcgtcaccc gcccgagcgc cgccggctcc ccaccggcaa ccgcccagac
    23761 ctgctcgagc gcctcgcgcg gagtcgtcat cggtgcacct cgccggaggt gggcaggcgg
    23821 gcggggggcg gggagtgggt cgggtgaccc gttcctgcca atcgaaccga agccgccgac
    23881 aacccagatt gggaactact ctcagggtga ccggagaaca gcgcgtcgtg gtcagagacc
    23941 cactccccgc cccccgcccg cctgcccacc gtcagatcac gccccgcttg gcgagatccg
    24001 cgatctggtc gggcgagtag ccgatcgccg tgaggatctc ctcgttgtcc tgccccagct
    24061 ccggcgccgc catgcgaatg ggc
//
LOCUS       DASUFA010000122         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1397415, whole genome shotgun sequence.
ACCESSION   DASUFA010000122 DASUFA010000000
VERSION     DASUFA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397415"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFX87_04480"
     CDS             <1..247
                     /locus_tag="VFX87_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994199.1"
                     /translation="NASIPIVTIVGIELGTLLGGSVITETIFAWPGVGRLSVQAIFNR
                     DYPVVQSAVFLLASTFVIVNFLVDVVYTYLDPRIRYR"
     gene            247..1203
                     /locus_tag="VFX87_04485"
     CDS             247..1203
                     /locus_tag="VFX87_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994200.1"
                     /translation="MAALPPSTPADEVQLHPAAAPEIAPPGRPRPWTEREWVTLLRRL
                     VRRRTALFGMLVVLLVMLGAVAAPLVAPFDPLEQDIGQRLREPGWQDAQGRVHALGTD
                     HLGRDILARIIFGSRIALLVGLAAVMISGVLGMVIGLVAGYFGGRMDDFLMRLADIQL
                     AFPFILLAIAVIGVLGPSLRNIIIVIGVSSWVVYARVVRGEVLSIREREYVHAAVALG
                     SRHWRVLVTHVLPNTFTPWLVVATLDMARVIVIESALSFLGLGVQPPTPTWGGMLADG
                     RVYLSTAWWLATFPGLAILITVLGINLFGDGLRDTLDPRLKV"
     gene            1214..2407
                     /locus_tag="VFX87_04490"
     CDS             1214..2407
                     /locus_tag="VFX87_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994201.1"
                     /translation="MDTSDTLVLSGARIIDGTGAEPVRGRSVVVEKGVITAVVEDARA
                     PRGNGVDLAGHTLLPGLINCHVHLCLGAEADPVRPLREEPLALTAIKALLRARETARA
                     GVTTVRDLGGREYVEIAARRAIQEGLIDGPRILAAGRPVCMTGGHGHWLGREADGPDD
                     ARKAVREQLKAGADVIKIIATGGVMTPGVEPGSPQMTFEEMRAAIEEARKAGRRTAAH
                     AMAATGISEAIDAGITSIEHGVFLTEEIVAHMRRDGTFLVPTLNAPSAIATGGLAAGI
                     PPFMVRKSEQVVPAHVASFQLAHRAGVRIAAGADSGTPLNFHGSLLPELALMVKYGMT
                     PLEAIRSATVTAAACLGLGEVTGRVAPGYAADLIAVAGDPAERIEALADLKLVLVNGR
                     AIEGG"
     gene            complement(2410..3879)
                     /locus_tag="VFX87_04495"
     CDS             complement(2410..3879)
                     /locus_tag="VFX87_04495"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX4994202.1"
                     /translation="MSDLPAWRQGDLPHAPAYTPINLVRVIGPGAILLGVSLGAGDWL
                     LGPAVAAAYGPALLWICTLSILFQGVLNAEMARYTLATGEPIFTGFMRQRPGARFWAA
                     GYSLLHLAQVGWPGWALAGGSALAALLLGRVPRAEDREVVVGLGYLIFLGAVGLVLLG
                     RRAQRALEITEWIMMAWMLLSLVALAVRFVPGRAWAVLAWSFAGRPLWEPGWSPSGFP
                     AATLDWALVAAFAAYSGAGGTINAMLTYWLRDKGFGMAGTVGAQPTRVGDQTILLPRD
                     GAIFPPSEANLAKWREWWRYLRADLSYLWTAGCLIGMGLPVLLALDAVPAGTDMGGFG
                     AGAVFARELTRRYWAVLWLPALLTTLWIFFSTQVGIVEAFARNVTEMLWTAGVRPATA
                     GAGWVYYPVLVLLVVAGCLALTVGDPLTLIVIGANIAAVNFAVLSFHTLRLNRALLPE
                     ALRPPLWREAIVAVGGLGFTALALLAAAARLGLLVAPGG"
     gene            complement(3876..4454)
                     /locus_tag="VFX87_04500"
     CDS             complement(3876..4454)
                     /locus_tag="VFX87_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX4994203.1"
                     /translation="MTTARRFLPPLAWSALIAWFSGAGWHGGATTPLLEPWLRALLPW
                     ATLEQIAGAHWLLRKTGHVVEYAVLALLWRRALAGPDAPATWRWPLALVALTAGLDEI
                     HQALTPMREGRVADVLLDSGAAAAALLVATLGARRAADGLAWILLWAAAAGGTAMLAI
                     DLAASAPSGWLWLSVPAAWLALWVWRRRRAGA"
     gene            4519..>5112
                     /locus_tag="VFX87_04505"
     CDS             4519..>5112
                     /locus_tag="VFX87_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4994204.1"
                     /translation="MQIRELLDELLTSLETGPLITAVRHFDAKPAVFAPFPSSLDPRI
                     VEALKARGVQQLYSHQAKAFDTVAKGEHLVVVTPTASGKTLCYNLPVLQALVQQPEAR
                     VLYLFPTKALAQDQLAELMELSKSLPDMRMFTYDGDTPQDARRSVRARANLVLTNPDM
                     LHSGILPHHTKWVNLFQNLKYVVIDELHAYRGVFGSHL"
BASE COUNT          749 a         1851 c         1860 g          652 t
ORIGIN      
        1 gaacgcgtcg atcccgatcg tcaccatcgt ggggatcgag ctcggcacgc tgctcggcgg
       61 ctccgtgatc accgagacca tcttcgcgtg gccgggggtg gggcggctct cggtccaggc
      121 catcttcaac cgtgactatc cggtcgtgca gtcggcggtg ttcctgctgg ccagcacctt
      181 cgtgatcgtc aacttcctgg tcgacgtggt ctacacctac ctcgatccgc gcatccggta
      241 ccgctgatgg ccgcgctgcc gccctccact cccgcggacg aggtgcagct ccatcccgcc
      301 gccgcccccg agatcgcgcc gccggggcgc ccgcgcccct ggacggagcg cgagtgggtg
      361 acgctgctgc ggcggctggt gcggcgccgg accgcgctct tcggcatgct cgtggtgcta
      421 cttgtgatgc tgggcgcggt ggccgctccg ctcgtcgcgc cgttcgatcc gctggagcag
      481 gacatcggcc agcgcctgcg ggagccgggc tggcaggacg cgcaggggcg cgtgcacgcg
      541 ctggggaccg accacctcgg gcgcgacatc ctggcgcgca tcatcttcgg ctcgcgcatc
      601 gcgctgctgg tgggactggc cgcggtcatg atctcgggcg tgctcggcat ggtcatcggg
      661 ctcgtggccg gctacttcgg cgggcgcatg gacgacttcc tgatgcgcct ggccgacatc
      721 cagctcgcct tcccgttcat cctgctcgcg atcgcggtca tcggggtgct ggggccgagc
      781 ctgcgcaaca tcatcatcgt catcggggtg tcgtcctggg tggtctacgc gcgggtggtg
      841 cgcggcgagg tgctctcgat ccgcgagcgc gagtacgtgc acgcggcggt ggcgctgggc
      901 agccggcact ggcgggtgct cgtcacccac gtgctgccga acaccttcac gccctggctg
      961 gtggtggcca cgctggacat ggcgcgcgtg atcgtgatcg agtccgcgct ctcgttcctg
     1021 gggctgggcg tgcagccgcc cacgcccacc tggggcggca tgctcgccga cggccgcgtc
     1081 tacctctcga ccgcgtggtg gctggccacg ttcccgggtc tcgcgatcct gatcaccgtg
     1141 ctcggcatca acctcttcgg cgacgggctc cgcgacacgc tcgacccgcg cctcaaagtc
     1201 tgacccgagg cctatggaca cctccgacac gctggtgctg agcggggcac ggatcatcga
     1261 cgggaccggg gcggagcccg tgcgcgggcg atcggtcgtg gtcgagaagg gcgtgatcac
     1321 cgcggtggtg gaggacgcgc gggcgccgcg aggcaacggg gtggacctgg cggggcacac
     1381 gctcctgccc gggctcatca actgccacgt ccacctctgc ctcggcgcgg aggccgatcc
     1441 ggtgcggccg ctgcgcgagg agccgctggc gctcaccgcc atcaaggcgc tgctgcgcgc
     1501 gcgtgagacg gcgcgcgccg gggtgaccac ggtgcgggac ctgggcggcc gcgaatacgt
     1561 ggagatcgcg gcgcggcgcg ccatccagga ggggctcatc gacgggccgc gtatcctggc
     1621 ggccggccgc ccggtgtgca tgaccggcgg gcacgggcac tggctcggcc gcgaggccga
     1681 cgggcccgac gacgcgcgga aggcggtgcg cgagcagctc aaggcgggcg ccgacgtgat
     1741 caagatcatc gccaccggtg gcgtgatgac cccgggtgtc gagcccggct ctccccagat
     1801 gaccttcgag gagatgcgcg cggccatcga ggaggcgcgg aaggcggggc ggcgcaccgc
     1861 ggcccacgcc atggccgcga cggggatcag cgaggcgatc gacgcgggga tcacctcgat
     1921 cgagcacggc gtcttcctca ccgaggagat cgtggcccac atgcggcgag acgggacctt
     1981 cctggttccc accctgaacg cgcccagcgc catcgcgacg ggcggactcg cggcggggat
     2041 ccccccgttc atggtgcgca agtccgagca ggtcgtgccc gcccacgtgg cgagcttcca
     2101 gctcgcgcat cgcgcgggcg tgcggatcgc ggcgggcgcc gactcgggca ccccgctcaa
     2161 cttccacggc tcgctgctgc ccgagctcgc gctgatggtg aagtacggca tgaccccgct
     2221 cgaggcgatc cgatccgcca ccgtcaccgc ggccgcgtgc ctgggcctgg gcgaggtgac
     2281 cggccgcgtg gccccgggct acgcggcgga tctgatcgcg gtggcggggg atccggccga
     2341 gcgcatcgag gcgctcgccg atctcaagct cgtgctggtg aacggccgcg cgatcgaagg
     2401 agggtaggct cagccgccgg gcgcgacgag cagcccgagc cgggccgccg ccgcgaggag
     2461 ggccagcgcc gtgaacccga ggccgcccac cgccacgatc gcctcgcgcc agagcggagg
     2521 gcgcagcgcc tccggcagca gcgcgcgatt caggcgcagc gtgtggaagg acagcaccgc
     2581 gaagttcacc gccgcgatgt tggcgccgat cacgatcagg gtgagcgggt cgcccaccgt
     2641 gagggcgagg catcccgcca ccaccaagag cacgagcacc ggatagtaga cccagcccgc
     2701 gcccgcggtg gccgggcgca cgcccgccgt ccagagcatc tcggtcacgt tgcgcgcgaa
     2761 ggcctccacg atgccgacct gggtcgagaa gaagatccag agcgtggtca gcagcgcggg
     2821 cagccagagc acggcccagt agcgccgggt cagctcgcgc gcgaagaccg cgcccgcccc
     2881 gaagccgccc atgtcggtgc ccgccggcac cgcgtcgagg gcgagcagca ccggcagccc
     2941 catgccgatc aggcagccgg ccgtccacag ataggagagg tcggcccgga gatagcgcca
     3001 ccactcgcgc cacttggcga ggttcgcctc gctgggcggg aagatggccc cgtcgcgcgg
     3061 caacaggatg gtctgatcgc ccacccgcgt gggctgcgcc cccacggtgc ccgccatccc
     3121 gaagcccttg tcgcgcagcc agtaggtgag catggcgttg atcgtgccgc ccgcgccgga
     3181 ataggccgcg aaggccgcga cgagcgccca gtcgagcgtg gccgccggga agcccgacgg
     3241 gctccagccc ggctcccaca gcggacggcc cgcgaagctc cacgcgagca ccgcccaggc
     3301 ccgcccgggc acgaaacgga cggccagcgc cacgagggac agcagcatcc acgccatcat
     3361 gatccactcg gtgatctcga gggcgcgctg ggcgcgccga ccgaggagca cgagcccgac
     3421 cgcgccgagg aagatcagat acccgaggcc gaccaccacc tcgcggtctt cggcacgcgg
     3481 cacgcggccg agcagcagcg cggccagcgc ggagccgccc gcgagcgccc agcccggcca
     3541 gcccacctgg gccaggtgca ggagcgagta gccggcggcc cagaagcgtg cgcccggccg
     3601 ctggcgcatg aagcccgtga agatgggctc gccggtggcc agggtgtagc gcgccatctc
     3661 cgcgttgagc acgccctgga agagaatgga cagcgtgcag atccagagca gggccgggcc
     3721 gtaggcggcc gcgacggccg ggccgagtag ccagtcgccc gcccccagcg agaccccgag
     3781 caggatcgcg cccggcccga tgacccggac caggttgatg ggcgtgtagg cgggcgcgtg
     3841 cggcaggtcg ccctgccgcc acgcgggcag atcgctcacg cgccggcgcg cctccgccgc
     3901 cagacccaga gggcgagcca cgccgcgggg acgctcagcc agagccagcc ggacggcgcg
     3961 gaggcggcca gatcgatcgc gagcatcgcg gtgccgccgg cggcggccgc ccagagcagg
     4021 atccacgcga gcccgtcggc ggcgcggcgc gccccgagcg tggccaccag gagggcggcc
     4081 gccgccgcgc cgctgtcgag gagcacgtcg gcgacgcgcc cttcacgcat cggcgtgagg
     4141 gcctggtgga tctcgtcgag cccggcggtg agcgcgacga gcgcgagcgg ccaccgccag
     4201 gtggcaggcg cgtcggggcc ggccagggca cggcgccaga gcagggcgag caccgcgtac
     4261 tcgacgacgt ggccggtctt gcgcaggagc cagtgggctc ctgcgatctg ctcgagggtg
     4321 gcccagggca agagcgcgcg cagccagggc tccagcagcg gggtcgtggc gccgccgtgc
     4381 catccggcgc ccgagaacca cgcgatcagc gctgaccatg cgagcggcgg caggaaccgg
     4441 cgggccgtcg tcacaggctc cagcgtgtca caggggcccg aagttgacaa cttgcgctca
     4501 ctaaacatag gatgagccat gcaaatccgg gagctcctcg atgagctctt gacgtcgctc
     4561 gagaccggcc cgctcatcac cgccgttcgg cacttcgacg ccaaacccgc cgtcttcgcg
     4621 ccgtttccgt cctcgctgga cccccgcatc gtggaggcgc tcaaggcgcg cggcgtccag
     4681 cagctctact cgcatcaggc caaggccttc gacacggtgg ccaagggcga gcacctcgtg
     4741 gtggtgacgc cgaccgcctc cggcaagacc ctctgctaca acctgcccgt gctgcaggcg
     4801 ctcgtgcagc agccggaggc gcgggtgctc tacctcttcc ccaccaaggc gctcgcgcag
     4861 gatcagctcg ccgagctgat ggagctgtcg aagtcgctgc ccgacatgcg gatgttcacc
     4921 tacgacggcg acaccccgca ggacgcgcgc cgctcggtgc gcgcgcgggc gaacctggtg
     4981 ctgaccaatc ccgacatgct gcactccggg atcctgccgc accacaccaa gtgggtgaat
     5041 ctcttccaga acctcaagta cgtcgtgatc gacgagctgc acgcctaccg gggcgtcttc
     5101 ggcagccatc tc
//
LOCUS       DASUFA010000123        10291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1060843, whole genome shotgun sequence.
ACCESSION   DASUFA010000123 DASUFA010000000
VERSION     DASUFA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060843"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="pgk"
                     /locus_tag="VFX87_04510"
     CDS             complement(<1..570)
                     /gene="pgk"
                     /locus_tag="VFX87_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX4994205.1"
                     /translation="MAKLVVDQLDLSGKRVFLRADFNVPLEGARVTDDTRITAVRPTI
                     DCCLKGGASLILASHLGRPKGKPDPKYSLRPVAERIEALLGRPVPLLPDCVGPEVEER
                     ARALRPGEVVLLENLRFHAEEEANDDAFAQSLAGLADVYVNDAFAAAHRAHASTEGIA
                     RHLKPAAAGLLMARELDALGRIFERPERPL"
     gene            complement(599..1603)
                     /gene="gap"
                     /locus_tag="VFX87_04515"
     CDS             complement(599..1603)
                     /gene="gap"
                     /locus_tag="VFX87_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX4994206.1"
                     /translation="MAVRVGVNGFGRIGRVFFRAALESSDLEVVAVNDLADAKTLAHL
                     LKHDSVHGTLNAEVKAKGEAIFVNGREVRVCSAKDPATLPWGEMGVEIVVESTGVFRD
                     RAMTSKHLQAGAKKVVITAPAKDPDITVVLGVNEGKYDPVKHELVSNASCTTNCLATV
                     AKVLLDNFGITRGFASTVHSYTNDQPIQDFPHKDLRRARAGAVSMIPTTTGAATAVGL
                     VLPELKGKLDGIAIRVPTVNVSVVDLTVELGKTVTPAVVNDAFRAAAAGPLKGILDAT
                     DEELVSVDFNGNPHSSIVDLPSTAVVDGNLVKVLAWYDNEWGYSSRVRDLIRYMARSL
                     "
     gene            complement(1715..2176)
                     /locus_tag="VFX87_04520"
     CDS             complement(1715..2176)
                     /locus_tag="VFX87_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX4994207.1"
                     /translation="MREALHAFKFRGRRALAAPLGDLLVEVMEGRPPAEVPALLVPVP
                     LHPRREWERGFNQASLLARRVGRACRLPVRDDVLVRAVATPSQTELDAAARRANVRGA
                     FRLRRPELIAGRQVLLVDDILTTGATLSECARCLREGGAATVGALTVARVV"
     gene            complement(2512..3549)
                     /locus_tag="VFX87_04525"
     CDS             complement(2512..3549)
                     /locus_tag="VFX87_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994208.1"
                     /translation="MSIRVGLVVGLLFAIVVTYLTSINPSRVHVALGGDWAFDIPLMA
                     LIAVVFGAGAGLALVLGILRDVTRSYARRTDPLEGAPRIAPPAPARAAADAPGEAARP
                     ATAQAGRDALIERRRFAEALEIQARVVQAAPRDERAAEGTVLAGLHYEIGRERLEKND
                     RAGAISHLKDALRAQPDFVPAALLLGEAHLKAGEPREALRVWERAAEGPQPALPVLSR
                     IEQLHRAEGRPTRMISLYQNALARQPDNLALAFGLGRVYFELAMLDEAAEQFQKMEVR
                     APELPLIHAYLGAILERRGQFRDAMEEYRRALRESDRIEWPHRCAACGAAHARWVDRC
                     PACRRWNTSRP"
     gene            complement(3571..3936)
                     /locus_tag="VFX87_04530"
     CDS             complement(3571..3936)
                     /locus_tag="VFX87_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX4994209.1"
                     /translation="MKKDRLVQLRKKLEEKHRQLEEEVGRNVLYGKGPEDDSIKDLGD
                     QASSAYNREFLFELGNGERRRLKEVALALQKITEGGFGDCERCGEPISDKRLDAVPDA
                     RYCIECQRAIEEEERTAAR"
     gene            complement(4006..4374)
                     /gene="rsfS"
                     /locus_tag="VFX87_04535"
     CDS             complement(4006..4374)
                     /gene="rsfS"
                     /locus_tag="VFX87_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX4994210.1"
                     /translation="MAILSAEAVVRVAARAALEKKAIDLVVLDLQGLSSVADFFIVCS
                     ARSTPQADTIADAVRGALRAEGVRPRHNEGSAESGWLLLDYGDVVVHVFLEETRGFYS
                     LERLWGDAPLVSVEGAGARD"
     gene            complement(4374..5039)
                     /gene="nadD"
                     /locus_tag="VFX87_04540"
     CDS             complement(4374..5039)
                     /gene="nadD"
                     /locus_tag="VFX87_04540"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX4994211.1"
                     /translation="MSGSSRLQPRVGIFGGSFNPIHFGHLLVADEVCEVLRLDRLLLV
                     PAAQPPHKPAAELAPAVHRYRMTVLAVREHPRFEVSDVEVTRSGPSYTVDTLAALQDR
                     GRLHLVIGSETFLDLLNWREPRRVAALARLVVIPRNGMGFDPETPAALKVMRELGLPG
                     FVSAGVEPSDDPAPILLHAASLPISGSDLRRRAREGRSLAFRMPEAVVSYIRDQQLYR
                     PEA"
     gene            complement(5026..6297)
                     /locus_tag="VFX87_04545"
     CDS             complement(5026..6297)
                     /locus_tag="VFX87_04545"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX4994212.1"
                     /translation="MGEAGMDAAAHVEAKARAAKEAARVLALAPSRAKNEALAQMARG
                     LEEKSGAILEANRQDLERARAAGHPRAFVDRLTLTESRIEEMAAGLRHVAGLPDPVGE
                     TVETWRRPSGLEIARVRVPLGVIGFIYESRPNVTADAAGLCLKSGNAVLLRGGSEALE
                     SNTMLAQILARAVEKAGLPPEIIQAVETADRAAVLAMLTLDRYLDLIIPRGGEEFVRL
                     VAERSTVPVLKHDKGLCHLYVDEGADLDMAVAVAVNAKAQRVSVCNSLETLLVHAAVA
                     PAFLPRLAARLAEAGVETRADERARALMPGAREAEPADWDTEYLDYILAVRVVESFED
                     AVAHIRRHGSGLAESIVTRDLRRARRFTQEVDAAAVLVNASTRLVDGSQFGMGAEMGI
                     STSRVHARGPVGVRELTTTKFVVWGDGHIRE"
     gene            complement(6333..7415)
                     /gene="proB"
                     /locus_tag="VFX87_04550"
     CDS             complement(6333..7415)
                     /gene="proB"
                     /locus_tag="VFX87_04550"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX4994213.1"
                     /translation="MKVGSGLVTTPGEGPSSETIARLAGDLAALVQDKREVALVSSGA
                     IATGVARLGLKGRPQSIPEKQAAAAVGQSALMWEYEQAFKKHGIPVGQVLLTGQDISD
                     RSRYLNARNTLLALLDYGVLPVINENDTVAVDEIKVGDNDNLAALVAHLIDADLLVLL
                     TDVDGLYTGDPRRDPGARRLETVEAITDEIQKMVFDASASVSVGGMSTKLEAAQKAGA
                     SGIPMVIASGREAGILQRLLKGEPVGTYFQPRDDRLAARKRWIAFAVPPQGRLTVDAG
                     AKKALTERGTSLLPSGLVEVSGEFRVGEVVALAQLDGQEFARGLVNYDAGELRRIRGA
                     KTKDIEKVLGYKGVDEVIHRDNLVIL"
     gene            complement(7436..8452)
                     /gene="obgE"
                     /locus_tag="VFX87_04555"
     CDS             complement(7436..8452)
                     /gene="obgE"
                     /locus_tag="VFX87_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX4994214.1"
                     /translation="MFVDEIDVFVKGGDGGAGCVSFRREKYVPRGGPDGGDGGDGGSV
                     ILEADPSITTLLDFHYQRHYTAERGQHGKGSNKQGASGDDTVLRVPLGTMVSERDGRL
                     PLGDLTTPGERLQVARGARGGRGNARFATSTNRAPRRADLGRAGEERWIHLELKLLAD
                     VGVIGFPNAGKSTLVSRLSAAKPKIADYPFTTLVPSLGIVRAGEDQSFVIADLPGLIP
                     GAAEGKGLGHRFLRHTERTRVLVHLIDLDPSTGRDPLADYQAIQDELHAYSEELAARP
                     QIVTVNKVELPGTEAGRAAVERFCAANGLTFHAISAVTGEGLPTLVHEMARRLAREPW
                     VTAH"
     gene            complement(8486..8746)
                     /gene="rpmA"
                     /locus_tag="VFX87_04560"
     CDS             complement(8486..8746)
                     /gene="rpmA"
                     /locus_tag="VFX87_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX4994215.1"
                     /translation="MAHKKGVGSSRNGRDSNPQMLGVKRFGGQFVTAGSILVRQRGTR
                     FKAGANVGLGVDHTLFAKADGYVRFERKGDDKYISVAQQQPV"
     gene            complement(8750..9061)
                     /gene="rplU"
                     /locus_tag="VFX87_04565"
     CDS             complement(8750..9061)
                     /gene="rplU"
                     /locus_tag="VFX87_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX4994216.1"
                     /translation="MEAVIATGGKQYRVAPGQVIRVERLGRDKGAAVEFTKVLLVNRD
                     GQVTIEPKALASAKVTGEVVAERRGAKVLVVKFKRRKNYRRHRGHRQTMTAVRITKIE
                     V"
     gene            complement(9216..9857)
                     /locus_tag="VFX87_04570"
     CDS             complement(9216..9857)
                     /locus_tag="VFX87_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4994217.1"
                     /translation="MTTLHAQNGHQDTAGGPKKVDRDAALVERLRRQEPGAPEELIAT
                     YGDRVYRLAIRITSNEQDAEEVVQDALWTAARKIDTFKGESAFGSWVYRITANAAYQK
                     LRSRHGQRNEISWDALTPGFDELGQHAEPLRDWSGRTEEPALQTELRKLLTTAIDDLP
                     GDYRTAFLLHDVEGLSNPEIAETLHISLPAVKSRVHRSRLFLRQRLGEYMRTA"
     gene            complement(9854..>10291)
                     /locus_tag="VFX87_04575"
     CDS             complement(9854..>10291)
                     /locus_tag="VFX87_04575"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX4994218.1"
                     /translation="AIIDRIRRAFVERGLGPAFADADTDPVGQLHAFFDRVLDTQRQR
                     NCVGGCAMGNLASELSDVHEGFRQQLAGIFDVYRDHLAETIRRGQGSGGLRPDVDAAR
                     VAQFLVAGLEGAILLGKVTRDITVIERCVEELKAHLTLYRNRA"
BASE COUNT         1481 a         3722 c         3552 g         1536 t
ORIGIN      
        1 gagcggccgc tcgggccgct cgaagatccg cccgagcgca tccagctcgc gcgccatgag
       61 cagccctgcg gccgccggct tcagatggcg ggcgatgccc tcggtggagg cgtgggcgcg
      121 atgggccgcc gcgaaggcat cgttgacgta gacgtccgcc agcccggcga gcgactgcgc
      181 gaaggcgtcg tcgttggctt cttcctccgc gtgaaagcgg aggttctcga ggagcaccac
      241 ctcacccggc cggagggcgc gcgcgcgctc ctccacctcc ggccccacgc aatccgggag
      301 caggggcacc gggcggccca ggagcgcttc gatccgctcg gccaccgggc gcagcgagta
      361 cttcggatcg ggcttgccct tgggacgccc gagatgggag gcgaggatca gcgaagcacc
      421 ccccttgagg cagcagtcga tggtcggccg caccgcggtg atccgcgtgt cgtcggtcac
      481 ccgcgcgccc tccaggggca cgttgaagtc ggcccgcagg aagacgcgct tcccggacag
      541 atcgagctga tcgacgacga gcttcgccac ggatggggcg gcgcggccgc gggccgggct
      601 agagcgaccg ggccatgtag cggatcaggt cgcgcacccg ggaggagtag ccccactcgt
      661 tgtcgtacca tgcgagcacc ttgaccaggt tgccgtccac gaccgccgtc gaaggcagat
      721 ccacgatgga ggaatgcgga ttgccgttga agtccaccga caccagctcc tcgtcggtgg
      781 cgtccaggat gcccttgagc gggccggcgg cggcggcgcg gaacgcgtcg ttgaccacgg
      841 ccggagtgac cgtcttgccg agctccaccg tgaggtcgac caccgagacg ttgacggtcg
      901 ggacccggat cgcgatgccg tcgagcttgc ccttcagctc gggcagcacc aggcccaccg
      961 ccgtcgcggc cccggtggtg gtggggatca tgctcaccgc gcccgcacgg gcgcggcgca
     1021 ggtccttgtg cgggaagtcc tggatcggct ggtcgttggt ataggagtgc acggtcgagg
     1081 cgaagccgcg cgtgatgccg aagttgtcga gcaggacctt cgccaccgtg gccaggcagt
     1141 tggtcgtgca ggaggcgttc gagaccagct cgtgcttgac cggatcgtac ttgccctcgt
     1201 tgacaccgag cacgaccgtg atgtccgggt ccttggcggg cgcggtgatc acgaccttct
     1261 tggcgcccgc ctgcaggtgc ttggacgtca tggcccgatc ccggaagacg ccggtggatt
     1321 ccaccacgat ctccacgccc atctcgcccc acggcagcgt ggccggatcc ttggcggagc
     1381 agacgcggac ctcccggccg ttgacgaaga tggcctcgcc cttggccttc acctcggcgt
     1441 tcagcgtgcc gtggacggaa tcgtgcttca ggaggtgggc cagcgtcttg gcgtcggcca
     1501 gatcattgac cgccaccacc tcgagatccg aagactcgag cgccgcgcga aagaaaaccc
     1561 ggccgatgcg tccgaacccg ttcaccccaa cacgcactgc catgggtgct cccttccctg
     1621 tttgtctcgg tttgtcaaat acttccggtc cgcggaggac tttgattata gcgagcctgg
     1681 gcgccgaggc gcaagcatgc ccctccgccc ggggctagac caccctggcc accgtcagcg
     1741 ccccgaccgt ggcggccccg ccctcccgga ggcagcgggc gcactcggag agggtcgcgc
     1801 ccgtggtcag gatatcgtcc acgaggagca cctggcgacc ggcgatgagc tccggacggc
     1861 gcagccggaa ggcgcctcgc acgttcgccc gccgggccgc agcgtcgagc tcggtctgcg
     1921 atggggtggc caccgcgcgg accagcacgt cgtcgcggac cgggagccgg caggcccgcc
     1981 ccactcgccg cgccagcagc gaggcctggt tgaagccgcg ctcccactcg cgtcgcgggt
     2041 gcagcggcac cggcacgagc agagcgggaa cctcggccgg cggccggcct tccatcacct
     2101 cgaccagcag atccccgagt ggcgccgcca gggcccgccg gccccggaac ttgaacgcgt
     2161 gcagcgcctc ccgcatcacg ccctcgtagc gcgccgcgct ccgggcatag tcaaaccccg
     2221 gcgtccgcct ccggcactgg ccgcagagcg gcccgcgccc ggaacgagtg tccgcagact
     2281 gggggagtgc gggggccatt tccgggcccc cgcacataaa aaagggcata ccgcatacgc
     2341 ggcaccacgg tggccgtatc cgcaccaccg ccaaccaaca agcaccacac agcggatccc
     2401 gccgcccgac atccagccgc accccgcaca ccggacagaa cgggggaaag acaaggtcca
     2461 gcagaacccc ggcccatcgg caccagcggc cggcgtcggg acgcgcgcgt tctagggcct
     2521 ggacgtgttc cagcgacggc aggcgggaca acggtcaacc cagcgggcat gggcggcgcc
     2581 gcacgccgcg cagcgatgcg gccattcgat gcgatcgctt tctcggagcg cgcgccgata
     2641 ctcctccatc gcgtcccgga actgcccgcg ccgctccagg atcgcgccga ggtaggcatg
     2701 gatgaggggc agctcgggcg cgcgcacctc catcttctgg aactgctccg cggcctcgtc
     2761 gagcatcgcg agctcgaagt agacgcggcc cagcccgaag gccagcgcca gattgtcggg
     2821 ctggcgggcg agcgcgttct ggtagagcga gatcatgcgg gtgggccggc cctcggcgcg
     2881 gtgcagctgc tcgatgcgcg acagcaccgg cagggcgggc tgcgggccct ccgccgcccg
     2941 ctcccagacg cggagcgcct cgcgcggctc gcccgccttg aggtgggcct cgcccaggag
     3001 cagggccgcg ggcacgaagt cgggctgggc ccgcagggcg tccttcaggt gcgagatcgc
     3061 gccggcgcga tcgtttttct cgagccgctc ccggccgatc tcgtagtgca gcccggccag
     3121 cacggttcct tcggccgccc gctcgtcccg cggggcggcc tgcaccacgc gcgcctggat
     3181 ctccagggcc tcggcgaagc gccggcgctc gatcagcgcg tcgcgcccgg cctgggcggt
     3241 cgcggggcgt gccgcctcac cgggagcgtc ggccgccgcg cgggcgggag ccggcggcgc
     3301 gatgcgcggc gccccctcga gaggatcggt ccggcgcgcg taggagcgcg tgacgtcgcg
     3361 aaggatgccg agcaccaggg ccaggcccgc gcccgcgccg aacacgaccg cgatcagcgc
     3421 catcaggggg atgtcgaagg cccagtcgcc gccgagggcg acgtgcacgc gactgggatt
     3481 gatggaggtc agataggtga cgacaatggc gaagagaagg ccgacgacga gcccgacgcg
     3541 gatcgacacg aacgaagcgg agcggcggcg ctagcgggcc gcggtgcgct cttcctcttc
     3601 gatcgcccgc tggcactcga tgcagtaccg cgcgtccggc accgcgtcca ggcgcttgtc
     3661 ggagatcggc tcgccgcagc gctcgcagtc gccgaacccg ccttcggtga tcttctgcag
     3721 cgcgagcgcg acttccttca gcctccggcg ctcgccgttg cccagctcga agaggaactc
     3781 gcgattgtag gcggaagagg cctggtctcc caggtccttg atcgaatcgt cttcggggcc
     3841 ctttccataa aggacgttcc ggcccacctc ctcctcgagc tggcggtgct tctcctccag
     3901 ctttttgcgg agctgaacca aacggtcttt cttcatctgt ggcctcctgg cggttcacgc
     3961 ggcgtcgccg ctagtcgcgc aggctaccat ggggtcctac gcaaatcaat cccgggcgcc
     4021 cgctccctcg accgaaacga gcggcgcgtc gccccacagc cgctccagcg agtagaatcc
     4081 gcgcgtctcc tcgagaaaca cgtgcaccac cacgtcgccg tagtcgagca ggagccagcc
     4141 gctctcggcg ctgccctcgt tgtggcgcgg ccgcacgcct tctgcgcgca gcgcgccgcg
     4201 taccgcgtcc gcgatggtgt cggcctgggg cgtggaccgg gcgctacaca cgatgaagaa
     4261 gtcggccacg ctcgacagcc cctgcaggtc cagcaccacc agatcgatcg ccttcttctc
     4321 cagggccgcg cgggcggcca cgcggacgac ggcttcggcg gacaggatgg ccatcaggcc
     4381 tccggacggt agagctgctg gtcgcggatg taggacacca ctgcctcggg catgcggaag
     4441 gcgaggctcc ggccctcgcg ggcgcgccgg cgcaggtccg agcccgagat gggcagggac
     4501 gccgcgtgca gcaggatcgg cgcggggtcg tccgacggct cgacccccgc ggacacgaag
     4561 ccgggcaggc ccagctcccg catcaccttg agggcggcgg gggtctcggg gtcgaaaccc
     4621 atcccgttgc gcggaatcac caccaggcgc gcgagggccg cgacccgccg cggctcgcgc
     4681 cagttcagca gatcgaggaa cgtctcggat ccgatgacga ggtgcagtcg cccccgatcc
     4741 tgcagcgcgg ccagggtatc caccgtgtag gacgggccgc tccgcgtgac ctcgacgtcg
     4801 gagacctcga agcgcgggtg ctcgcgcacc gcgagcacgg tcatccggta gcgatggacg
     4861 gccggcgcca gctcggccgc cggcttgtga ggcggttgcg ccgccggcac cagcagcagg
     4921 cgatcgagac ggaggacttc gcagacttcg tcggccacga ggaggtggcc gaaatggatc
     4981 gggttgaacg atcccccgaa gatgccgacg cgcggctgca ggcggctact cccggatatg
     5041 cccgtcgccc cacaccacga acttggtggt cgtcagctcg cgcaccccga ccggaccccg
     5101 ggcgtggacg cgggaagtgg agatgcccat ctccgcgccc atcccgaact ggctgccgtc
     5161 cacgagccgg gtcgacgcgt tgacgagcac cgccgccgcg tccacctcct gggtgaagcg
     5221 ccgcgcgcgc cgcaggtcac gggtgacgat ggactcggcc agccccgagc cgtgccgccg
     5281 gatgtgggcg accgcatcct cgaagctctc caccacccgc accgccagga tgtagtcgag
     5341 gtactccgtg tcccagtccg cgggctcggc ctcccgggcc ccgggcatca gggcccgtgc
     5401 ccgctcatcc gcgcgggtct ccacgcccgc ctccgcgagc cgggcggcca gccgcgggag
     5461 gaacgcgggc gcgacggccg cgtgcacgag cagcgtctcc agcgagttgc agacgctgac
     5521 tcgctgggcc ttggcgttga ccgccaccgc caccgccatg tcgagatccg cgccctcgtc
     5581 cacgtacagg tggcagagcc ccttgtcgtg cttgagcacc gggaccgtcg agcgctccgc
     5641 gacgagccgt acgaactcct cgccaccccg cgggatgatc aggtcgaggt agcggtccag
     5701 cgtgagcatg gcgagcaccg ccgcgcggtc cgccgtctcc accgcctgga tgatctcggg
     5761 cgggagcccc gccttctcga cggcccgggc gaggatctgg gccagcatgg tgttggactc
     5821 gagcgcctcg ctgccgcccc gcaacagcac cgcgttgccg gacttgagac agaggccggc
     5881 cgcgtccgcg gtcacgttcg gccgtgactc gtagatgaag ccgatcaccc ccagggggac
     5941 gcgcacgcgc gcgatctcga ggccggaggg ccggcgccag gtctccacgg tctcgccgac
     6001 cggatcgggg aggccggcca cgtggcgcag gcccgccgcc atctcctcga tgcggctctc
     6061 ggtgagggtg aggcggtcca cgaaggcgcg cggatggccc gcggcacggg cgcgctcgag
     6121 atcctgccga ttggcctcga ggatcgcgcc cgacttctcc tcgagcccgc gcgccatctg
     6181 ggcaagggcc tcgttcttgg cccggctcgg agccagcgcg agcacccggg ccgcctcctt
     6241 ggccgcccgc gccttcgcct cgacatgtgc cgccgcgtcc atgcctgcct ctcccatcgg
     6301 tgagcggttg cctgcctctc cccttcccgc tttcagagga tcaccaggtt gtcccggtgg
     6361 atcacctcgt cgacaccctt gtagcccagg accttctcga tgtccttcgt cttcgcgccc
     6421 cggatccgcc gcagctcccc cgcgtcgtag ttcacgaggc cgcgcgcgaa ctcctggcca
     6481 tcgagctgag ccagcgccac cacctcgccc acgcgaaact ccccgctcac ctccaccagg
     6541 ccggagggaa gcaggctcgt acccctctca gttaaggcct tcttcgcgcc cgcgtcaacc
     6601 gtgagccgcc cctggggcgg caccgcgaag gcgatccacc gcttgcgcgc ggcgagacgg
     6661 tcgtcccgcg gctggaagta cgtcccgacc ggctcgccct tgagcagccg ctggaggatc
     6721 ccggcctcgc gcccgctcgc gatcaccata gggatgccgg aggcgcccgc cttctgcgcc
     6781 gcctccagct tggtgctcat gccgccgacc gacacgctag cggaggcgtc gaagaccatc
     6841 ttctggatct cgtcggtgat ggcctcgacg gtctcgaggc ggcgggcccc cggatcgcgg
     6901 cggggatcgc cggtatagag cccgtccacg tcggtcagga gcacgagcag gtcggcgtcg
     6961 atgaggtgcg cgaccagggc ggcgaggttg tcgttgtcgc ccaccttgat ctcgtccacc
     7021 gccaccgtgt cgttctcgtt gatcaccggg agcaccccgt agtcgagcag ggcgagcagc
     7081 gtgttgcggg cgttgagata gcgcgagcga tcgctgatgt cctgtccggt gagcagcacc
     7141 tggcccaccg ggatgccgtg cttcttgaac gcctgctcgt actcccacat cagcgcggac
     7201 tgccccaccg ctgcggccgc ctgcttctcc gggatcgact ggggccggcc cttgaggccg
     7261 aggcgcgcca cgccggtggc gatggcgccc gaggacacga gcgccacttc acgcttatcc
     7321 tggacgaggg cggccaggtc tccggcgaga cgggcgatcg tctcgctcga cgggccttcc
     7381 cccggcgtgg tgacgagccc gctgccgacc ttcacgacta tccgacgagc gtgcctcagt
     7441 gggcggtcac ccatggctcc ctcgccagac gccgggccat ctcgtggacc agcgtgggca
     7501 ggccttcccc ggtcaccgcg gagatggcgt gaaaggtcag gccgtttgcc gcgcagaagc
     7561 gctcgaccgc cgcgcgcccc gcctcggtgc ccggcagctc caccttgttc acggtcacga
     7621 tctgcgggcg cgcggccagc tcctccgagt acgcgtgcag ctcgtcctgg atcgcctggt
     7681 agtcggccag cggatcgcgc ccggtggagg gatcgagatc gatgaggtgc acgagcaccc
     7741 gcgtgcgctc ggtgtggcgc aggaagcgat gtccgaggcc tttcccttcc gccgcgccgg
     7801 gaatgaggcc gggaagatcg gcgatgacga agctctgatc ctcaccggcc cgcacgatgc
     7861 cgagcgaggg cacgagcgtg gtgaaggggt agtcggcgat cttgggcttg gccgccgaga
     7921 gccgggagac cagcgtggac ttgccggcgt tcgggaagcc gatcaccccc acgtcggcga
     7981 ggagcttcag ctcgaggtgg atccagcgct cctcgcccgc ccggccgaga tccgcgcggc
     8041 gcggggcgcg gttcgtcgag gtggcgaagc gcgcgttgcc gcgcccgccg cgggcccccc
     8101 gcgccacctg cagccgctcg ccgggcgtgg tgaggtcgcc caggggcagg cgcccgtccc
     8161 gctcggagac catggtgccg agcggcacac gcaggacggt gtcgtcgccg ctcgcgccct
     8221 gcttgttgga gcccttgccg tgctgcccgc gctcggcggt gtagtgccgc tgatagtgga
     8281 agtccagcag ggtggtgatg gaaggatccg cttcgaggat cacgctgccg ccgtccccgc
     8341 catcgccgcc atcgggaccg ccgcggggga cgtacttctc acgccggaag ctgacgcagc
     8401 ccgcgccgcc atcgcccccc ttgacgaaga catcgatctc gtcgacgaac atggcgtggc
     8461 ggcagtccgg cggtgccgga tcggctcaga caggctgctg ctgggcgacg ctgatgtact
     8521 tgtcgtcgcc cttgcgctcg aagcgcacgt agccgtcggc cttggcgaag agcgtgtggt
     8581 ccacgcccag gcccacgttc gcgccagcct tgaagcgggt cccacgctgc cgcaccagga
     8641 tgctgccggc ggtgacgaac tgcccgccga agcgcttcac ccccagcatc tggggattgg
     8701 agtcgcgacc gttccgggag ctgccgacac ccttcttgtg agccatggcc tagacctcga
     8761 tcttggtgat gcgcaccgcg gtcatggtct gccgatgccc gcgatgacgc cggtagttct
     8821 tccgccgctt gaacttcacg accaggacct tggcgccccg gcgctcggcc accacttcgc
     8881 cggtgacctt ggcggaggcg agtgccttcg gttcgattgt cacctgccca tcccggttga
     8941 ccaggaggac cttggtgaat tccaccgcag cgcccttgtc tcggcccagc cgctcgaccc
     9001 ggatcacctg ccccggagcg acgcggtact gcttgccccc tgtcgcgatc accgcttcca
     9061 tgccgccgac ctttccattg cgtggtgcat ctcgtgtctg tctcgtggtt gccgctcgtg
     9121 ggaccacact gcgtcaatcg cgcggactcg ccgcgagcga ctatctgacc acaggctccc
     9181 gccgaaagtc aagctggacg gggctttagg cgccctcagg cggtccgcat gtattcgccc
     9241 agtcgctggc ggaggaacag ccgcgagcgg tgcacgcgcg acttcacggc gggcaggctg
     9301 atgtgcagcg tctcggcgat ctccgggttg gagagcccct ccacgtcgtg cagcaggaac
     9361 gcggtccggt agtcgcccgg cagatcgtcg atggcggtgg tcaacagctt gcgcagctcg
     9421 gtctggagcg ccggctcctc ggtgcgcccc gaccagtcgc ggagcggctc cgcgtgctgg
     9481 ccgagctcgt cgaagcccgg cgtcagggca tcccaggaga tctcgttgcg ctggccatgc
     9541 cggctgcgga gcttctggta ggccgcgttc gcggtgatgc ggtagaccca gctcccgaag
     9601 gcggactcgc ccttgaaggt gtcgatcttg cgggcggccg tccagagcgc gtcctgcacc
     9661 acttcctccg cgtcctgctc gttgctcgtg atgcggatgg cgagccggta gacccggtcg
     9721 ccataggtgg cgatcaactc ctccggcgcg ccgggctcct gccgccgcaa gcgctccacc
     9781 agcgcggcgt cccgatccac cttcttcggg cctcctgcgg tgtcctgatg tccgttctgt
     9841 gcgtgcagcg tcgtcatgcc cgattcctgt agagcgtgag gtgcgccttc agctcctcga
     9901 cgcatcgttc gatgaccgtg atgtcccggg tcaccttgcc caggagaatg gcgccttcga
     9961 gtcctgccac gaggaactgg gccacccgcg cggcgtccac atccgggcgg agcccgccgc
    10021 tgccctggcc acgccggatc gtctccgcga gatggtcccg gtagacgtcg aagatgccgg
    10081 ccagctgctg gcggaagccc tcgtgcacgt cggagagctc ggaggcgaga ttgcccatcg
    10141 cgcagccgcc cacgcagttg cgctgccgct gcgtgtcgag cacgcgatcg aagaaggcgt
    10201 ggagctggcc gaccgggtcg gtgtccgcgt ccgcgaaggc gggcccgagg ccccgctcga
    10261 cgaaggcgcg ccggatccga tcgatgatcg c
//
LOCUS       DASUFA010000124         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_143159, whole genome shotgun sequence.
ACCESSION   DASUFA010000124 DASUFA010000000
VERSION     DASUFA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143159"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /gene="acsA"
                     /locus_tag="VFX87_04580"
     CDS             <1..1632
                     /gene="acsA"
                     /locus_tag="VFX87_04580"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX4994219.1"
                     /translation="IAHEAVDRHLARGHGEHVALRWLGKRGETVDYTYAGLAELTSRF
                     ANVLRSLGVGPGERVYALAGRIPELYVAALGTLKSRGVFCPMFSAFGPEPIRVRMATG
                     QARVLVTTESLYRRKVAGIRAALPDLRHVLLVGDDRGPTGVPGTLDYVTLMARADGRF
                     DIERTDPEEMALLHFTSGTTGRPKGAVHVHEAVVAHHITGRLALDLHPEDVFWCTADP
                     GWVTGTSYGIIAPLTCGITSIVDEAEFDAARWYRILQEQRVTVWYTAPTAIRMLMKTG
                     AALARQHDLRALRFLASVGEPLNPEAVVWGQEAFGRPFHDNWWQTETGGIMIANYATM
                     DIRPGSMGRPLPGVEAAIVRKLGEDRVEVIEEADAQGELALRPGWPSMFRGYWQEPER
                     YRKCFAGGFYLTGDLARRDRDGYFWFVGRADDVIKSSGHLIGPFEVESVLMEHKAVAE
                     AGVIGKPDPVAMEVVKAFVSLKDGYEPTEELRRDLLGFARSRLGAVVAPKEIAFQASL
                     PKTRSGKIMRRLLRARELGLPEGDTSTLEGAEAER"
     gene            1629..>2240
                     /locus_tag="VFX87_04585"
     CDS             1629..>2240
                     /locus_tag="VFX87_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4994220.1"
                     /translation="MTDIDRAHALGLLRQMLLIRRFEEKAAELYSVGKIRGFLHLYIG
                     EEAVAVGTMQALTPADGIVATYREHGQALARGIPAGSLMAEMYGKANGCSRGRGGSMH
                     FFDVSRRFYGGHAIVGGGLPVAVGLALADKLKALARVTACFFGDGAVAEGEFHESLNL
                     AALWKLPVLFLCENNLYAMGTAVARHQSQPDIRRKADAYGIASD"
BASE COUNT          322 a          788 c          807 g          323 t
ORIGIN      
        1 atcgcccacg aggcggtcga ccgtcacctg gctcgcggcc acggggagca cgtggcgctc
       61 cggtggctcg gcaagcgcgg cgagaccgtc gactatacct acgccggcct cgccgagctg
      121 acgagccgct tcgccaacgt cctgcgctcg ctcggcgtgg gcccgggcga gcgtgtctac
      181 gccctggccg gacgcattcc cgagctctac gtcgccgcgc tcggcacgct caagagccgc
      241 ggggtgttct gcccgatgtt ctcggcgttc ggtcccgagc ccatccgggt ccgcatggcc
      301 accggccagg cccgcgtgct cgtcaccacg gagtcgctct accggcgtaa ggtcgccggg
      361 atccgggcgg cgctcccgga tctccggcac gtgcttctgg tcggcgacga ccgcgggccc
      421 accggggttc cgggaaccct ggactacgtc accctcatgg cccgcgccga cgggcgattc
      481 gacatcgagc ggacggaccc ggaggagatg gcgctgctcc atttcaccag cggcaccacc
      541 ggccggccca agggcgcggt gcacgtgcac gaggcggtcg tcgcccacca catcacgggg
      601 cggctcgcgc tcgatctcca tccggaggat gtcttctggt gcaccgcgga tccgggctgg
      661 gtcaccggaa cgtcctacgg gatcatcgcg ccgctgacct gcgggatcac cagcatcgtg
      721 gacgaggccg agttcgacgc cgcccgctgg taccgcatcc tgcaggagca gcgcgtgacg
      781 gtctggtaca ccgcgccgac cgcgatccgc atgttgatga agacgggcgc ggcgctcgcg
      841 cggcagcacg atctccgggc gctgcggttc ctcgccagcg tgggagagcc gctcaacccg
      901 gaggcggtcg tctgggggca ggaagccttc ggccggccgt tccacgacaa ctggtggcag
      961 accgaaacgg gcgggatcat gatcgccaac tacgcgacca tggacatccg cccgggctcc
     1021 atgggccgcc cgctgccggg cgtggaggcc gcgatcgtcc ggaagctcgg cgaggatcgg
     1081 gtggaggtca tcgaggaggc cgacgcgcag ggcgagctgg cgctccgccc cgggtggccc
     1141 tccatgttcc gcgggtactg gcaggagccc gagcgctacc ggaagtgctt cgcgggcggc
     1201 ttctatctca cgggcgacct cgcccggcgg gatcgcgacg gctacttctg gttcgtcggg
     1261 cgggccgacg acgtgatcaa gagctccggc cacctcatcg gcccgttcga ggtggagagc
     1321 gtgctgatgg agcacaaggc ggtggccgag gccggtgtca tcggcaagcc cgacccggtg
     1381 gcgatggaag tggtcaaggc cttcgtctcg ctcaaggatg gctacgagcc gaccgaggag
     1441 ctccgccgcg acctgctggg cttcgcgcgc tcccggctcg gggcggtggt cgcgccgaag
     1501 gagatcgcct tccaggcgag cctgcccaag acccggagcg gcaagatcat gcggcgcctg
     1561 ctccgagccc gtgagctggg gctgcccgag ggagacacct cgacgctcga gggcgcggag
     1621 gccgagcgat gaccgacatc gatcgagcgc acgccctggg cctgctccgc cagatgctgc
     1681 tgatccggcg gttcgaggag aaggccgccg agctctacag cgtgggcaag atccgcggct
     1741 tcctgcacct ctacatcggc gaggaggccg tggcggtggg gaccatgcag gcgctcaccc
     1801 cggcggacgg gatcgtggcg acctaccgcg agcacggtca ggccctggcg cgagggatcc
     1861 cggccggctc actcatggcc gagatgtacg gcaaggccaa cggctgcagc cgcgggcgcg
     1921 gcggctccat gcacttcttc gacgtctcgc gccggttcta cggcggccac gccatcgtcg
     1981 gcggcggcct gcccgtcgcg gtggggctcg cgctcgccga caagctgaag gccctggcgc
     2041 gggtgaccgc gtgcttcttc ggcgacggag cggtggccga gggcgagttc cacgagtccc
     2101 tcaatctggc cgcgctgtgg aagctcccgg tcctcttcct ctgcgagaac aacctctacg
     2161 cgatgggcac cgcggtggcc cgccaccagt ctcagcccga catccggcgg aaggccgacg
     2221 cctacggcat cgcgtcggac
//
LOCUS       DASUFA010000125         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_30015, whole genome shotgun sequence.
ACCESSION   DASUFA010000125 DASUFA010000000
VERSION     DASUFA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30015"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VFX87_04590"
     CDS             <1..901
                     /locus_tag="VFX87_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994221.1"
                     /translation="AFGLGLEEEEEPVSLGLKGSISDFEGSAEYRSIGKRLERLVSGP
                     PSQRDREGTEVWVGQRLGLLRLRLSQSDLADNVDRNPALPRTTRGQTAITAQVAPRAW
                     PVFGLTYAKGDSERMWLTGDGRPRATEEQTFDSMSGSAYYGAPRWDVSAFSTFIKSRE
                     VARPDHEMTMLYHDLRFTLRPIDALVVVPAVSTGLDRYEWSDTRSSNGSASLLLSFGP
                     PGSWWKLWTYGAYTASQSSDRTVDGRTLSLSGGLSCGLGRLLGGATTLSFQAGYERYD
                     DAVYPESSARGAFGLVRLRVAAF"
     gene            complement(923..1780)
                     /locus_tag="VFX87_04595"
     CDS             complement(923..1780)
                     /locus_tag="VFX87_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994222.1"
                     /translation="MIARPAVAGIDTRRAPRNRLAWAAAAVIAAAVLAALLAPVLPLI
                     DPDVVDTPNRLRPPGAAGHWLGTDEFGRDMLARLVWGARISLLAGVGTATASMLIGVL
                     LGVHAGYWGGRVDTVVMRLTDILMAFPYILLAIAIVAGLGPGLTNAMVAIAIVGFPIY
                     TRLARGVVLSLREREFVEAARALGSTDRLILRRHLLPHLLAPVVVAWSLDVGAKILAT
                     AGLSFLGLGTQPPTADWGSMLATARTFVILSPHVALLPGLAIFLVVLSLNILGDALQD
                     YLDPRMRHG"
     gene            complement(1777..>2236)
                     /locus_tag="VFX87_04600"
     CDS             complement(1777..>2236)
                     /locus_tag="VFX87_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994223.1"
                     /translation="SGMWAPYGGGSARDLLAHLALPALTLAAASVTIIARLTRSTMLE
                     VLGQDYIRTARAKGLVERLVVTRHGLRNALIPIVTVVGVQAGYLLGGAIFTETVFAWP
                     GVGTLMIQGILARDFPLVQGCVLVIALVFVFFNLAVDLLYAVLDPRIRYE"
BASE COUNT          340 a          784 c          830 g          282 t
ORIGIN      
        1 ggccttcggc ctggggctgg aggaggaaga ggagccggtc tccctcgggc tcaagggctc
       61 gatcagcgac ttcgagggca gcgccgagta tcgctcgatc ggcaagcggc tcgagcgcct
      121 ggtgagcgga ccgccgtcgc agcgagaccg ggagggcacc gaggtctggg tcgggcagcg
      181 gctgggcctc ctgcgcctgc gcctgtcgca gtccgacctg gccgacaacg tggaccgcaa
      241 cccggcgcta ccccgcacca cccggggcca gaccgcgatc accgcgcagg tcgcgccgcg
      301 ggcctggccg gtcttcggac tgacctacgc caagggcgat tcggagcgca tgtggctgac
      361 cggggatggc cgcccgcggg ccaccgagga gcagaccttc gatagcatgt ccggcagcgc
      421 ctactacggc gcgccgcgct gggatgtctc ggcgttctcc accttcatca agagtcgtga
      481 ggtggcgcgg cccgatcacg agatgaccat gctgtaccac gatctgcgct tcaccctgcg
      541 gccgatcgat gccctggtcg tcgtgccggc ggtgagcacc ggtctcgacc gctacgaatg
      601 gtcggacacc cgcagcagca acggctcggc ctcgctgctg ctgtccttcg ggccgcccgg
      661 gagctggtgg aagctgtgga cgtacggcgc ctacaccgcg tcgcagagca gcgaccgcac
      721 ggtggacggc cgcaccctga gcctgagcgg cgggctgtcg tgcgggctcg gccggctcct
      781 gggcggggcc accacgctct ccttccaggc cggctacgag cggtacgacg atgcggtgta
      841 ccccgagagt tccgcgcgcg gtgccttcgg ccttgtccgg ctccgggtcg ccgccttcta
      901 ggccctccgc ccgacgatcg cctcacccgt gccgcatgcg cgggtcgagg tagtcctgca
      961 gcgcgtcgcc gaggatattg agcgacagca ccaccaggaa gatggccagc cccggcagga
     1021 gcgccacgtg cgggctcagg atcacgaagg tgcgtgcggt cgcgagcatg cttccccagt
     1081 ccgcggtcgg tggctgcgtg ccgagcccca ggaaggacag ccccgcggtg gcgaggatct
     1141 tggccccgac gtcgaggctc cacgccacca ccacgggggc cagcaggtgg gggagcaggt
     1201 ggcggcgcag gatcagccgg tcggtgctgc cgagcgcgcg cgccgcctcg acgaactcgc
     1261 gctcgcgcag ggagagcacg acgccgcgcg cgagccgcgt gtagatcggg aagcccacga
     1321 tcgcgatggc caccatcgcg ttggtgaggc cggggccgag gccggccacg atcgcgatgg
     1381 cgagcaggat gtaggggaag gccatcagga tgtcggtcag gcgcatcacc accgtgtcca
     1441 cccgcccgcc ccagtagccc gcgtgcacgc ccagcagcac cccgatgagc atcgaggcgg
     1501 tggcggtgcc caccccggcc aggagcgaga tgcgcgcgcc ccacacaagg cgggcgagca
     1561 tgtcccggcc gaactcgtcg gtcccgagcc agtgcccggc ggcgccgggc ggcctgagcc
     1621 ggttcggggt gtccaccacg tcgggatcga tcaggggcag gacgggcgcg aggagtgcgg
     1681 ccagcaccgc ggccgcgatg accgcggcgg ccgcccacgc gaggcgattg cggggcgccc
     1741 ggcgcgtgtc gatcccggcc accgcggggc gcgcgatcat tcgtaccgga tgcggggatc
     1801 gagcaccgcg tagagcaggt ccaccgcgag gttgaagaag acgaagacga gcgcgatgac
     1861 cagcacgcag ccctgcacga gcgggaagtc gcgcgccagg atgccctgga tcatcagggt
     1921 gcccaccccc ggccacgcga aaacggtctc ggtgaagatg gcgccgccca gcaggtagcc
     1981 cgcctgcacg ccgacgacgg tgacgatggg aatcagcgcg ttgcgcaggc cgtggcgcgt
     2041 gaccacgagc cgctcgacga ggcccttggc gcgtgcggtg cggatgtagt cctggccgag
     2101 cacctccagc atcgtgctgc gcgtgaggcg cgcgatgatc gtcaccgacg ccgccgccag
     2161 cgtgagcgcg ggcagtgcca ggtgcgccag caggtcgcgc gcgctgccgc cgccgtaggg
     2221 cgcccacatt ccggag
//
LOCUS       DASUFA010000126         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_717184, whole genome shotgun sequence.
ACCESSION   DASUFA010000126 DASUFA010000000
VERSION     DASUFA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717184"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..3150
                     /locus_tag="VFX87_04605"
     CDS             97..3150
                     /locus_tag="VFX87_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX4994224.1"
                     /translation="MSQVGSSRYGAAVGAAGPPTPSSLDALRRVWPAYVVLACTLLLT
                     FGAWRYAERTVRANEQQRFGRVVARSRAAIDRRLESYLQILMGLRALFASSTAVERSE
                     FRSFTAGLGLDARYPSIRGVGWIPRVPAASRVAHEAVMRRGGAPGGYEIHPVESQAEF
                     HPVAFLEPTEAFEGRWLGFDLATQPENREAMERARDTGVAAMSRRLAPFRQSEGHGFV
                     IFLPVYRGGRLLSTVEERRQRFEGFVAAMFRPDLLMDELFGASRGEPVDLELFDAPEA
                     TPGRVIYDRDPSSADTPHELSHTVPVVFGGQTWTLHLATLPAFHSLAERLVPYWVLGS
                     ALLISVLAFVITLLQTRALTAARRLGADLQAAERRLRRANDRFELAASAVGSAIYDWN
                     LQHGTMTWSPGLTEVGGYRLDDAEPTYDWWSQRIHPGDRPRVEARFQAAAAGGDDFEA
                     EYRFRARDGHYLDVLNRGRFVRDAAGHAVRVVGSVADISERKRGESVLRDSEARHRAV
                     LETAMDAFVGMDHEGRVMEFNPAAERMFGHARADVMGRELAEVIVPPSFRDAHRRGLA
                     RYLVTGEHALLGGRIEVRACRADGTEFPVELTVTVVRMEGPPVFNAYIRDLTEQKRAD
                     AARSSLEMQLQQAQKMEAVGRLAGGVAHDFNNLLTVISGRAHMLMSRLKPGEPMHRDV
                     DLIQKTSQRAVALTSQLLAFSRKQVVQPRVLELGPLVADLGPMLQRMIGEDVELTVEP
                     VQGTGRVKADPSQMQQVLMNLAVNARDAMPAGGRIRVSVRDVLVDEAAALHQTNLPPG
                     PYVALAVSDTGTGMSAETAAHIFEPFFTTKEQGKGTGLGLSTVYGIVEQSRGHIQVES
                     ELGRGTTFTIHLPRTEEPLAAGPPADAARRLRTTSRTVLVVDDEPEVLELAGEILKRV
                     GYSVLEAADGAAALEVARLHEGEIHLLVTDMVMPGMSGRDLAARLRALRIALPVLYIS
                     GYVQDATARAALAGEHSAFVAKPFTPELLTDRVRELLATAEAGAG"
     gene            complement(3166..4839)
                     /locus_tag="VFX87_04610"
     CDS             complement(3166..4839)
                     /locus_tag="VFX87_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4994225.1"
                     /translation="MSFLSGKQAFLQILKDEGVDTMFGNPGTTELPLMDGLVREPSVR
                     YILALQEAVAISMADGYAQASGKLGVVNVHTSPGLGNAMGMLYDAYEAGTPLLVTAGQ
                     HDQAMNLTEPILWSDLPPVARPYVKWSHEITRLEDLPRAVRRAVKTAMAHPTGPVFIS
                     LPVDVLNAERELDLLQSTRVAPRIRGDRAAIEAAADLLVKAARPVLIAGDAVAHGDAL
                     AEMAQLAEVLGAPVYTECVPSTCSFPFTHPLYHGPFPRLGQPIRQLLMQHDLLFSVGG
                     DLFTLSLPSDVEPMPEGLPLIHLDLDPWEIGKNYPAQVAIQGDPKATLPELVEALKRR
                     LTASQAKDVSARVEKLRVAQEARREALRQEAAAQASRHPISPLALVGAVAGAVPDEAI
                     IVDETISSSHGVRELLRARDHRGFYGLRGGGIGWGLPASLGVKLAQPHRPVLALVGDG
                     SAMYTIQSLWSAAHDSIAVVFVIFNNQSYRILKQRTQALKGFSAEDNQYVAMDLVKPG
                     LDYVGLAKAQGVPGEMVEKTADVATAIKRGLASGGPYLVDVRIDGSLRG"
     gene            4919..>5075
                     /locus_tag="VFX87_04615"
     CDS             4919..>5075
                     /locus_tag="VFX87_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX4994226.1"
                     /translation="MPPAAPATFLDACLRRPTSVTPIWLMRQAGRYMPEYRAVRARLG
                     FLELCKSP"
BASE COUNT          704 a         1805 c         1845 g          721 t
ORIGIN      
        1 gtgcgcagtt gacggtatac ccgcggcccg gcacagcgca agagcgttgg tggcgcgcgc
       61 gccgcggagt aaaatcgaaa cgggcgcgac gcagccatga gtcaggtcgg atcgagtcgc
      121 tacggagccg cggtcggcgc agccggcccg cccaccccat cgtccctcga cgccctccgc
      181 cgcgtgtggc ccgcctacgt ggtgctcgcc tgcacgctgc tgctgacctt cggcgcgtgg
      241 cgctacgccg agcgcaccgt gcgggccaac gagcagcagc gcttcggccg ggtggtggcg
      301 aggagccgcg ccgcgatcga ccgaaggctc gagtcctacc tgcagatcct gatgggtctg
      361 cgcgctctct tcgcgagcag caccgcggtc gagcgctccg aatttcgctc cttcaccgcg
      421 ggcctgggtc tcgacgcgcg ctacccgagc atccggggcg tgggctggat tccgcgagtc
      481 cccgccgcgt ccagggttgc gcacgaggcg gtgatgcgcc ggggcggcgc gcccggcggc
      541 tacgagatcc acccggtgga gagccaggcc gagttccatc cggtggcgtt tctcgagccc
      601 accgaggcct tcgaggggcg ctggctcggc ttcgacctgg ccacgcagcc ggagaaccgc
      661 gaggccatgg agcgggcccg ggacaccggc gtcgcggcga tgagccggag gctcgccccg
      721 tttcgccaga gcgaggggca cggcttcgtc atcttcctcc ccgtgtatcg tggcgggcga
      781 ctactctcga cggtcgagga gcgccggcag cgcttcgaag gcttcgtggc cgccatgttc
      841 cggcccgacc tgctgatgga cgagctgttc ggcgcctccc gcggcgagcc ggtggatctg
      901 gagctcttcg acgcccccga ggcgaccccg ggccgtgtca tctacgaccg cgaccccagc
      961 tccgcggaca cgccgcacga gctgagccac acggtcccgg tcgtgttcgg gggccagacc
     1021 tggacgcttc acctcgcgac gttgcccgcc tttcattccc tcgccgagcg gctcgtaccc
     1081 tactgggtcc tgggcagcgc cctcctcatc agcgtcctcg ccttcgtgat cacgctgctg
     1141 cagacccggg ccctcaccgc ggcgcggcgg ctcggcgccg acctgcaggc ggccgagcgc
     1201 cggctgcgcc gggccaacga ccgcttcgag ctcgccgcat ccgcggtggg ctccgccatc
     1261 tacgactgga acctgcagca cggcacgatg acgtggtcgc cggggctcac cgaggtgggc
     1321 ggctatcgcc tggacgatgc cgagccgacg tatgactggt ggagccagcg gatccatccc
     1381 ggcgaccgac cgcgggtcga ggcgcgcttc caggcggccg cggccggcgg cgacgatttc
     1441 gaggcggagt accgcttccg cgcccgcgac ggccactacc tcgacgtgct gaaccgcggc
     1501 cggttcgtgc gcgatgcggc cggccacgcg gtgcgcgtgg tcgggagcgt ggccgacatc
     1561 tccgagcgga agcgggggga gtcggtgctg cgcgacagcg aggcccggca ccgcgcggtc
     1621 ctggaaaccg cgatggacgc cttcgtcggc atggaccacg aggggcgtgt gatggagttc
     1681 aatccggcgg ccgagcggat gttcgggcac gcgcgcgcgg acgtcatggg tcgggagctg
     1741 gcggaggtga tcgtcccgcc gtccttccgg gacgcgcacc ggcgcgggct ggcgcgctac
     1801 ctggtgaccg gcgagcacgc gcttctgggc ggccgcatcg aggtgcgcgc ctgccgggcc
     1861 gatgggaccg agttcccggt ggagctgacc gtgaccgtgg tgcggatgga agggccgccg
     1921 gtgttcaatg cctacatccg ggatctcacc gagcagaagc gggcggacgc ggcccggagc
     1981 agcctcgaga tgcagctcca gcaggcgcag aagatggagg cggtgggtcg cctcgcgggc
     2041 ggcgtcgccc acgacttcaa caacctgctg accgtgatca gcggccgggc ccacatgctg
     2101 atgagccggc tcaagccggg cgagccgatg caccgcgacg tggatctgat ccagaagacg
     2161 tcgcagcggg cggtggcgct caccagtcag ctcctggcct tcagccgcaa gcaggtcgtg
     2221 cagccgcgcg tgctggagct ggggccgctg gtggccgacc tggggccgat gctgcagcgg
     2281 atgatcggcg aggatgtcga gctgacggtc gagccggtgc agggcacggg gcgggtcaag
     2341 gccgatccca gccagatgca gcaggtgctg atgaacctcg cggtcaacgc ccgggacgcc
     2401 atgccggccg gcggccggat ccgcgtctcc gtccgcgacg tccttgtgga cgaggcggcc
     2461 gcgctccacc agaccaacct gccgccgggg ccctacgtgg cgctcgcggt cagcgatacc
     2521 ggtaccggca tgagcgcgga gacggccgcg cacatcttcg aaccgttttt taccaccaaa
     2581 gagcagggca agggcaccgg gctcgggctc tccaccgtgt acggcatcgt cgagcagagc
     2641 cggggccaca tccaggtcga gagcgagctc gggcgcggca ccacgttcac catccacctg
     2701 ccgcgcaccg aggagccgct cgccgccggc ccgcccgccg acgcggcgcg acgcctgcga
     2761 accacgtcac gaacagtcct ggtggtggac gacgagccgg aggtgctgga gctggccggc
     2821 gaaatcctga agcgcgtcgg ctactcggtg ctcgaggccg cggacggcgc cgccgcgctc
     2881 gaggtggcgc ggctccacga gggggagatc cacctgctcg tgaccgacat ggtgatgccc
     2941 ggcatgtcgg gccgcgacct ggccgcgcgg ctgcgcgcgc tgcggatcgc gctgccggtg
     3001 ctctacatct cgggctacgt gcaggacgcg accgcgcggg cggcgctggc cggcgagcac
     3061 tccgcgttcg tggcgaaacc cttcacgccc gagctcctca ccgaccgggt gcgcgagctc
     3121 ctggccaccg cggaggcggg ggccggctga gcccgacgcc cgcgctcagc cgcggagcga
     3181 gccgtcgatg cggacgtcca ccaggtaggg cccgccggag gcgagcccgc gcttgatcgc
     3241 ggtggccacg tcggcggtct tctccaccat ctcgccgggg acgccctgcg ccttcgcgag
     3301 ccccacgtag tcgagccccg gcttcaccag gtccatcgcc acgtactggt tgtcctccgc
     3361 cgagaacccc ttgagcgcct gggtgcgctg cttgaggatc cggtaggact ggttgttgaa
     3421 gatcacgaac accaccgcga tggaatcgtg cgccgccgac cacagcgact ggatcgtgta
     3481 catggcgctg ccgtcgccca ccagcgcgag caccggccgg tgcggctggg ccagcttcac
     3541 gccgagcgag gcgggtaggc cccagccgat gccgccgccg cgcagcccgt agaagccgcg
     3601 gtggtcgcgg gcccgcagca gctcgcgcac cccgtggctc gaggagatgg tctcgtccac
     3661 gatgatggcc tcgtcgggca ccgcgcccgc gaccgcgccc accagcgcga gcgggctgat
     3721 cgggtggcgg ctcgcctggg cggccgcctc ctggcgcagc gcctcgcggc gcgcctcctg
     3781 cgccacccgc agcttctcca cccgcgcgct cacgtccttg gcttgtgacg cggtcagccg
     3841 ccgcttgagc gcctccacca gctccggcag cgtcgccttc ggatcgccct ggatggcgac
     3901 ctgggccgga tagttcttgc cgatctccca ggggtcgagg tcgaggtgga tgagcggtag
     3961 cccttccggc atcggctcca cgtccgacgg cagggagagc gtgaagagat cgccgcccac
     4021 cgagaacagc aggtcgtgct gcatcaggag ctggcggatg ggctggccca ggcgcgggaa
     4081 cggaccgtgg tagagcgggt gcgtgaacgg gaaggagcag gtggagggca cgcactcggt
     4141 gtacaccggc gcgccgagca cctccgcgag ctgggccatc tcggccagcg cgtcgccgtg
     4201 ggccaccgcg tcgcccgcga tcaggacggg gcgcgccgcc ttcaccagca gatcggcggc
     4261 cgcctcgatc gcggcgcggt cgccgcggat gcgcggggcc acgcgcgtgg actgcagcag
     4321 atccagctcg cgctccgcgt tcagcacgtc gaccggcagc gagatgaaca ccggcccggt
     4381 gggatgcgcc atcgcggtct tcaccgcgcg gcggaccgcc cggggcaggt cctcgagccg
     4441 ggtgatctcg tgcgaccact tcacgtaggg ccgcgccacc gggggcagat cggaccagag
     4501 gatcggctcg gtgagattca tggcctgatc gtgctggccg gcggtcacga gcagcggcgt
     4561 ccccgcctcg taggcgtcgt agagcatgcc catcgcgttg ccgagccccg gcgaggtgtg
     4621 gacgttgacc acgcccagct tgccgctcgc ctgcgcgtag ccgtcggcca tgctgatggc
     4681 gaccgcttcc tgcagcgcca ggatgtagcg cacgctcggc tcccgcacga gcccgtccat
     4741 caggggcagc tcggtggtgc cgggattgcc gaacatggtg tccacacctt cgtccttcag
     4801 gatctgaagg aaggcctgct tgccggagag aaaggacatg gggggagcct cctcgctgcc
     4861 ggatcatagc cccgcttcct tgactgcgcc agtacccgcc tgtaggcttc ggggcagtat
     4921 gccgccggcc gctcccgcca cgttcctgga cgcgtgtctc cgacggccca cgtcggtcac
     4981 cccgatctgg ctcatgcggc aggccggccg ctacatgccg gagtaccgcg cggtgcgcgc
     5041 gcggctcggc tttctggagc tgtgcaagag cccgg
//
LOCUS       DASUFA010000127         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1519276, whole genome shotgun sequence.
ACCESSION   DASUFA010000127 DASUFA010000000
VERSION     DASUFA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519276"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFX87_04620"
     CDS             <1..348
                     /locus_tag="VFX87_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994227.1"
                     /translation="AAPEYDVAATRVILTQTPLAVLPMPTALRPLAAAGRRLLAARYL
                     AVYRKLRPLDRTRLPYFEAAGCMRGLVRAGEARVMEGVAPNQLDASSFGERLAARFGR
                     ASGVPVALPPRRR"
     gene            386..1792
                     /locus_tag="VFX87_04625"
     CDS             386..1792
                     /locus_tag="VFX87_04625"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4994228.1"
                     /translation="MNATDLAYAPATELIPQIRSKALSPVELTRAVLERIEQVNPKIN
                     AFCTVTADAALAAARAAEAAVMKGGPLGPLHGIPVSIKDLALVAGVPARFGSHVFANR
                     IGEVDAPYVRRLKEAGAILTGKTTTPEFGWKALGDSPLTGITRNPWNLGMTSGGSSAG
                     AGAATAAGLGPLHQGSDGAGSVRVPSAFCGIYGLKPTFGRVPMWPVSNTDSASHTGPM
                     TRTVADAALMLGVMAGPDDWDRQSLDAAPDDYVGRLREGVKGLRVGWSLDLGILQVDP
                     EVGQVAKRAALAFQELGCAVEDVKVSWPDTREMIHLMWNAHYAGNYGQYLDEFRSRMD
                     PGLVAALEDGRRYSAEAYVAMRGRKHVYCDAVRALFDTYDLLLTPTVSVAAFEVGRLN
                     PVGFPQHPWDWFPWAGFSYPFNFTGQPAATVPAGFTASGMPVGLQIVGRRLADLQVLQ
                     ASAAFEQVRPWAQKRPSV"
     gene            1765..2145
                     /locus_tag="VFX87_04630"
     CDS             1765..2145
                     /locus_tag="VFX87_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HEX4994229.1"
                     /translation="MGPEAAQRLSEWPPMWIMVAGPYRSGAATAEARARNLAELNRAA
                     LALFRAGHVPVIGVNLALPLIEAAGEAAFDEIMMPLSLRLVERCDACLRVGGPSQGAD
                     DEAARFRATGRPVYARLEEVAAVR"
BASE COUNT          295 a          830 c          860 g          320 t
ORIGIN      
        1 gccgcgccgg agtacgatgt ggccgcgacc cgcgtcatcc tcacccagac gccgctcgcc
       61 gtgctgccga tgcccaccgc gctccgcccg ctcgccgccg cgggtcgccg gctcctggcc
      121 gcgcgctacc tggccgtcta ccgcaagctg cgcccgctcg atcggacgcg gctgccgtac
      181 ttcgaggcgg ccgggtgcat gcggggactg gtgcgtgccg gggaggcgcg cgtgatggaa
      241 ggcgtggcgc cgaaccagct cgatgcctcg tccttcggcg agcggctcgc cgcgcgcttc
      301 gggcgcgcca gcggggtgcc ggtcgcgctg ccgccgcgcc gccgctgacc gtgctaccat
      361 cctcacgaac tgatccggag gccccatgaa cgcgaccgat ctcgcctacg cgcccgcaac
      421 cgagctcatc ccccagatcc ggtcgaaggc gctgtcgccg gtggagctga cccgggcggt
      481 gctcgagcgc atcgagcagg tgaacccgaa gatcaacgcg ttctgcacgg tgaccgcgga
      541 cgcggcgctg gccgccgcgc gcgccgccga ggcggcggtg atgaaggggg ggccgctcgg
      601 cccgctccac ggcatcccgg tctcgatcaa ggatctggcc ctggtggcgg gtgtgcccgc
      661 gcgcttcggc tcgcacgtct tcgccaaccg catcggcgag gtggacgcgc cgtacgtgcg
      721 ccggctcaag gaggcgggcg ccatcctcac cggcaagacc acgacgccgg agttcggctg
      781 gaaggcgctc ggcgacagcc cgctcaccgg gatcacccgc aatccgtgga acctgggcat
      841 gacctcgggc ggctcgagcg cgggggccgg cgcggccacc gcggcggggc tggggccgct
      901 ccaccagggc tcggacggcg ccggctcggt gcgggtgccg tcggcgttct gcggcatcta
      961 cgggctcaag cccaccttcg ggcgcgtgcc catgtggccc gtgtccaaca ccgacagcgc
     1021 ctcgcacacc gggcccatga cgcgcacggt ggcggatgcc gcgctgatgc tgggcgtgat
     1081 ggccgggccc gatgactggg accgccagtc gctcgacgcc gcgcccgacg actacgtggg
     1141 ccgcctgcgc gagggcgtga agggcctgcg cgtggggtgg agcctcgacc tggggatcct
     1201 gcaggtggat cccgaggtag ggcaggtggc gaagcgggcc gcgctcgcgt tccaggagct
     1261 gggctgcgcg gtcgaggacg tgaaggtgag ctggccggac acgcgagaga tgattcacct
     1321 gatgtggaac gcacactacg cgggcaacta cggccagtac ctcgacgagt tccgctcgcg
     1381 catggatccg ggcctggtcg cggccctcga ggacggccgc cgttacagcg cggaagccta
     1441 cgtggcgatg cgcggccgca agcacgtcta ctgcgacgcg gtgcgcgcgc tcttcgacac
     1501 ctacgatctg ctgctgaccc cgacggtgtc ggtcgcggcg ttcgaggtcg ggcgcctcaa
     1561 tccggtgggc ttcccgcagc atccgtggga ctggttcccg tgggccggct tcagctatcc
     1621 gttcaacttc accgggcagc ccgccgccac cgtgcccgcg ggcttcaccg cctcgggcat
     1681 gccggtgggg ctgcagatcg tcggccgccg cctggccgat ctccaggtcc tgcaggcctc
     1741 ggccgccttc gagcaggtgc ggccgtgggc ccagaagcgg cccagcgtct gagcgagtgg
     1801 ccgccgatgt ggatcatggt cgcgggaccg tatcgctccg gcgccgcgac cgcggaggcg
     1861 cgggcccgga acctggccga gctgaaccgc gccgcgctcg ccctgttccg cgccggccac
     1921 gtgccggtga tcggcgtgaa cctcgcgctg ccgctgatcg aggcggcggg cgaggccgcc
     1981 ttcgacgaga tcatgatgcc gctgtcgctc cgtctggtgg agcggtgcga cgcctgcctg
     2041 cgggtcggcg gcccgtcgca gggggccgac gacgaggccg cgcgcttccg ggccaccggg
     2101 cggccggtct acgcccggct cgaggaggtg gccgcagtcc gatgagcgcc gcgcggccct
     2161 acgacatcat caccttcgac tgctacggga ccctcatcga ctgggacggc ggcatcacgg
     2221 gggcgttcgc gaaggcggtc gcggagaccg gcggccggct cgatccaggc cacgtcttcg
     2281 aggcctacga gaagatcgag gcgga
//
LOCUS       DASUFA010000128         8061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_831834, whole genome shotgun sequence.
ACCESSION   DASUFA010000128 DASUFA010000000
VERSION     DASUFA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831834"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFX87_04635"
     CDS             complement(<1..285)
                     /locus_tag="VFX87_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX4994230.1"
                     /translation="MTTTAIIYSDEWRQFDYGPEHPLRMERLGLTWRLMEAYGLTALP
                     RAKVWAPERAELDEIARFHSREYIEILRAVSAGDWVPHAARYGLGPGDNPI"
     gene            complement(282..791)
                     /gene="moaC"
                     /locus_tag="VFX87_04640"
     CDS             complement(282..791)
                     /gene="moaC"
                     /locus_tag="VFX87_04640"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX4994231.1"
                     /translation="MSPSREMPGFRGLSHLTPAGEARMVDVSAKSETVREATARVTLK
                     MKAATLAAVRAGQLAKGDVLGVARTAAIMAAKRTPDLIPLCHPLRITGVDVEFALDSR
                     RSTITVHARVRTVDKTGVEMEALTAAAVAGLTVYDMVKAVDRGVILTDLCVVEKSGGK
                     SGTWTRRKS"
     gene            complement(812..1042)
                     /locus_tag="VFX87_04645"
     CDS             complement(812..1042)
                     /locus_tag="VFX87_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX4994232.1"
                     /translation="MSDTEAFIERLTAENHDFRTLREEHHRYEQELAALNTRGFLAPD
                     QQWRVSELKKLKLIAKDRMETLLRHARAATHA"
     gene            complement(1122..2219)
                     /locus_tag="VFX87_04650"
     CDS             complement(1122..2219)
                     /locus_tag="VFX87_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994233.1"
                     /translation="MTPLPVVALHGVQVSRAGRTILDIPELAVDAGQVLAVIGPNGAG
                     KSTLLRVMGLLEPATAGSVRFQGERVSPADGLAVRRRMASVFQEPLLADTTVRDNVAL
                     GLRFRGVDDGRAAPRVDAWLARLGIGHLAARQARTLSGGEAQRTALARALVLEPELLL
                     LDEPFSALDQPTREALLDDFARILRQERTTTVLVTHDRAEAMTLGDRVGVLMGGRLVQ
                     LDDAAQVFRAPASEDVARFVGVETILDGQVVEWTRDLALVDVAGQIVEVAQRAEPGER
                     VRLCVRPEDVTIFPGAPKPGGTREFNRLGGTVQRLMPSGPHVRVIIDCGFPLAALVTQ
                     RSVDEWGFVAGSPVTAHFKATAPHLLRHAKP"
     gene            complement(2216..3346)
                     /locus_tag="VFX87_04655"
     CDS             complement(2216..3346)
                     /locus_tag="VFX87_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4994234.1"
                     /translation="MTSRLEVSVRKTLPGFTLDVDWSAGDGVAVLFGPSGAGKTLTLQ
                     CLAGLVRPDAGRIVVNGQVLFDAVAGINLPPQRRRVGYVFQGYALFPHLSVRQNVGFG
                     LRDLARAARRRRVDEVLERLSIGAYADRFPRDLSGGQRQRVALGRALAIDPALLLLDE
                     PLSALDLPLRQALRDELRSILTGWGTAAVLVTHDLTEAYRLGDRIVVYEEGRVIQAAP
                     RSELLWQPSSRSVARIMGLRNVLQGVTLKATPDRIQFRWRGQMLEAVNSPTHSYLPAA
                     DSPLAFFIRPEYVRLIRKDRDGVDSAHHMNLMTGTIVREVDFGTTWTLYFRLDEPGAP
                     AQGDYDLEVEVPRLVYEILEIDRDRHWRFSLHRGSIHVLPSA"
     gene            complement(3343..4008)
                     /gene="modB"
                     /locus_tag="VFX87_04660"
     CDS             complement(3343..4008)
                     /gene="modB"
                     /locus_tag="VFX87_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX4994235.1"
                     /translation="MSPDNLVALRLSLQVAVAATALNALVGIPIAYVLARRRFWGRSL
                     VDLLVTLPLVLPPTVVGYYLIVLLGRRGVLGAPLYELSGFTVAFTWYAAVIAATVMAL
                     PLLVRTVRAAIESVDRDLERAAYTLGRSELRTAIEVTLPLARNGILAGLVLAFARALG
                     EFGATLMLAGNIPGRTATVPLSIYTAVQTGESGEALGLVAALTVLSCLVLVAANRLGA
                     RAA"
     gene            complement(4094..4864)
                     /gene="modA"
                     /locus_tag="VFX87_04665"
     CDS             complement(4094..4864)
                     /gene="modA"
                     /locus_tag="VFX87_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4994236.1"
                     /translation="MKRWIPVTLAVLLGLASAADAQSLTVSAAISMKDAVTEIGRGFT
                     AARPGVTLLYNWGSSGELQKQIEAGGPIDLFISAAVVQMDELEKRGLIVPGTRRVFAR
                     NLLTVIKPADSRVDIAKPTDLAGARVARIVVGNPRAVPAGQYAEESLRALGLWDRVQP
                     KLIFAENVRQALEYVARGEVDAGFVYATDVALRGQAVKEAFRPAEDTYRPIAYPAAVV
                     AGTRQPALAQAFIELLLSREGRAVLGRFGFQPPPAGVR"
     gene            complement(4911..5606)
                     /locus_tag="VFX87_04670"
     CDS             complement(4911..5606)
                     /locus_tag="VFX87_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994237.1"
                     /translation="MGLFGQGVLEAFRLLLAGDAEVWQITWLSLQVSAGATALSLLVG
                     IPLGALLALARFPGRALVVSLVNTGMGLPPVVVGLFVTILLWRNGALGFLELLYTPSA
                     MVTGQFVIAAPIVTGLTLAAVQQVPETFRLQMLGLGASRGQMLWVLLKEARLPMLAAV
                     MAGFGGVISEVGASMMLGGNIRGSTRVLTTATVLETGKGNFDLAIALSVILLLITFLV
                     NWALSLIQQRRRA"
     gene            complement(5624..6466)
                     /locus_tag="VFX87_04675"
     CDS             complement(5624..6466)
                     /locus_tag="VFX87_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX4994238.1"
                     /translation="MITRLAAVLSLALAGLLPSTTGPAVAQSNVVILSTTTSTQDSGL
                     LDVLVPMFEKQTGLTVKAISVGTGQALALAARGEADVTLAHAPGLEKKYMDEGKMTNR
                     RLVMYNDFVVIGPPDDPARIKGLPRALDALRRIAESRSRFVSRGDKSGTHTLELALWK
                     QAGVEPKGAWYIESGQGMGQTLGIANDRRAYALTDRATFLAFQKRVDLPILVEKDRPL
                     LNIYSVMEVNPANGPRVNARGGKAFADFMLAPETQAVIKSFGVDKYGQPLFVPVAGKK
                     DEDL"
     gene            6604..7464
                     /locus_tag="VFX87_04680"
     CDS             6604..7464
                     /locus_tag="VFX87_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX4994239.1"
                     /translation="MRRFDLVLPNSLEDCLKILAEHGGRAKLVAGGTDLLPQMKNGAL
                     HPALVVDLSGIARVRQVQNGSGLRIGAAVTARELELSPALRGPYLAIAESAAMVGSLQ
                     VRNLATVGGNLCNAAPSADMAPPLMALEAQAVIAGPGGERRVPMADFFTGVRKTVLAP
                     NELLVELIVPTPGAHSGGQYLRHTPRRELDIAVVGVASQLTMADGRCTKARIALASVA
                     PTPVRATAAERSLEGQAVTPEAIERAATLAIEAARPIDDQRGSIEFRKHLVRVLTRRT
                     LTTALQRANA"
     gene            7482..7973
                     /locus_tag="VFX87_04685"
     CDS             7482..7973
                     /locus_tag="VFX87_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4994240.1"
                     /translation="MAKQLLRCTVNGEAVEVLVQPYVNLLDALRENLGLTGPKEGCGT
                     GDCGACTVHVDGRVVASCLMLAMQARGRKVRTIEGLASADTTLHPLQEAFVRHGVPQC
                     GFCIPGVLMAAAALLEENPRPSEEEIRYGIAGNLCRCTGYTAMVTAISEAAETLASQR
                     SAR"
BASE COUNT         1238 a         2884 c         2851 g         1088 t
ORIGIN      
        1 gatcgggttg tcgccggggc cgagcccgta gcgggcggcg tgcggcaccc agtcgcccgc
       61 gctcaccgcg cgcaggatct cgatgtactc gcgcgagtgg aagcgggcga tctcgtcgag
      121 ctcggcgcgc tcgggggccc agaccttggc gcgcggcagc gcggtgaggc cgtaggcctc
      181 catgagccgc cacgtgagcc cgagccgctc catgcggagc ggatgctccg gcccgtagtc
      241 gaactggcgc cactcgtcgg aatagatgat tgcggtggtg gtcatgattt cctgcgggtc
      301 caggtaccgg acttgccgcc agatttttca acgacgcaga ggtcggtcag gatgacccct
      361 cgatcgacgg ccttcaccat atcgtacacg gtgaggccgg ccaccgcggc ggcggtcagc
      421 gcttccatct ccacgccggt cttgtccacc gtgcgcaccc gcgcgtgcac cgtgatcgtg
      481 gagcggcgcg agtcgagcgc gaactcgacg tccacgccgg tgatgcgcag cgggtggcag
      541 aggggaatca ggtcgggcgt gcgcttggcc gccatgatcg ccgcggtacg ggccaccccg
      601 agcacgtcgc ccttcgcaag ctggccggcg cgcaccgcgg ccagggtggc cgccttcatc
      661 ttgagggtga ctcgcgcggt cgcctcccgg acggtctccg acttggccga aacgtccacc
      721 atccgggctt cgccggcagg agtcaggtgg gagaggccac gaaagcccgg catctcccgg
      781 gacggggaca tcggtcgggc ttaaacccga ctcaggcgtg ggtcgcggcg cgcgcgtgcc
      841 ggagcagggt ctccatccgg tctttcgcga tgagcttgag cttcttcagc tcgctcacgc
      901 gccactgctg atcgggagcc aggaacccgc gcgtgttcag cgcggccagc tcctgctcgt
      961 agcggtggtg ctcttcacgg agggtgcgaa agtcgtgatt ctcggcggtg aggcgctcga
     1021 tgaaggcttc cgtgtcggac atgcggacct ccttgcgagg cggttgaccc tgccccaacc
     1081 agcgtcgtca tccgcgttat actccccggc tcttccgact gtcaaggctt ggcgtggcgc
     1141 aggagatggg gggcggtggc cttgaaatgc gcggtcaccg ggctgccggc gacgaacccc
     1201 cattcgtcca cggagcgttg cgtcacgagg gccgccagcg ggaacccgca gtcaatgatc
     1261 acacgaacat gtggaccact cggcatcagg cgctgcaccg tgccacccag ccggttgaac
     1321 tcccgcgtcc cgccgggctt gggggcaccg gggaagatgg tcacgtcctc cggcctcacg
     1381 cagagacgca cgcgctcgcc cggctcggcc cgctgcgcca cctcgacgat ctggcccgcg
     1441 acgtccacca gggccaagtc ccgggtccac tcgacgacct gcccgtcgag gatggtctcc
     1501 acccccacga atcgcgcgac gtcctcggag gcgggcgccc ggaacacctg ggccgcgtcg
     1561 tcgagctgca cgaggcggcc gcccatgagg acgccgaccc ggtcccccag cgtcatggcc
     1621 tcggcgcgat cgtgcgtcac cagcacggtg gtggtgcgct cctggcgcaa gatccgggcg
     1681 aagtcgtcga gcagcgcctc ccgggtgggc tggtccagcg ccgagaacgg ctcgtcgagc
     1741 aggagcagct cgggctcgag cacgagcgcg cgggccagcg cggtccgctg cgcctcgccc
     1801 cccgacagcg tgcgcgcctg gcgggccgcc agatgcccga tgccgaggcg cgcgagccac
     1861 gcgtccaccc gcggcgcggc gcgcccgtcg tccacccccc ggaagcggag gccgagggcc
     1921 acgttgtccc ggacggtggt gtcggcgagg aggggctcct ggaagacgct ggccatgcgc
     1981 cgccgcaccg cgaggccgtc ggccgggctc acccgctcgc cctggaaccg cacgctgccc
     2041 gcggtcgccg gctcgagcag gcccatcacg cggagcagcg tggacttgcc ggcgccgttg
     2101 gggccgatga ccgcgaggac ctggcccgcg tccaccgcca gctcggggat gtccagaatg
     2161 gttcttcccg cgcgagagac ctgcacgccg tgcagcgcca cgaccggtag cggcgtcatg
     2221 cggacggcag gacgtggatc gagccgcggt gcagtgagaa gcgccagtgc cggtcgcggt
     2281 cgatctcgag gatctcgtag acgagccgtg gcacctcgac ttccagatcg tagtcaccct
     2341 gcgcgggggc gccgggctca tcgagccgga agtagagcgt ccaggtggtg ccaaaatcca
     2401 cctcgcgcac gatggtcccg gtcatgaggt tcatgtgatg cgcggagtcc accccgtcgc
     2461 ggtccttgcg gatcagccgc acgtactccg ggcggatgaa gaacgcgagc gggctgtccg
     2521 ccgcgggcag gtacgagtgc gtcggcgagt tcaccgcctc gagcatctgc ccccgccagc
     2581 ggaactggat gcgatcgggc gtggccttga gcgtcacgcc ctggagcacg ttgcgcagcc
     2641 ccatgatgcg ggccacgctg cgcgaggacg gctgccagag cagctccgag cgcggggccg
     2701 cctggatcac gcggccctcc tcgtagacca cgatgcgatc gccgagccgg taggcctcgg
     2761 tcagatcgtg ggtcaccagc accgccgcgg tgccccagcc cgtgaggatg ctgcgcagct
     2821 cgtcccgcag cgcctggcgc agcggcaggt cgagcgcgga gagcggctcg tccagcagga
     2881 gcagcgccgg gtcgatggcg agcgcgcggc cgagggccac gcgctggcgc tggccacccg
     2941 acagatcgcg cggaaagcgg tccgcgtagg ccccgatcga gagacgctcc agcacctcgt
     3001 ccacccgccg ccggcgcgcg gcccgcgcga ggtcgcggag gccgaacccc acgttctggc
     3061 gcacgctcag atgcgggaac agcgcgtagc cctggaacac gtagccgacc cgtcgccgct
     3121 ggggcgggag attgatcccg gccaccgcgt cgaagagcac ctggccgttc accacgatgc
     3181 ggccagcgtc cggccggacg agcccggcca ggcattgcag ggtaagcgtc ttgccggctc
     3241 ccgaggggcc gaagagcacc gcgacgccgt cgcccgcgct ccagtcgacg tccagggtga
     3301 accccggcag cgtcttccgg accgagacct cgagacggct cgtcatgccg cgcgggcccc
     3361 caggcgattg gcggcgacga gcacgagaca ggacagcacc gtgagggccg cgaccaggcc
     3421 gagcgcctcg ccgctctcgc cggtctgcac cgcggtatag atggagaggg gcacggtcgc
     3481 ggtgcggccg gggatgttgc cggccagcat cagggtcgcg ccgaactcgc cgagcgcccg
     3541 cgcgaaggcc agcacgagcc cggccaggat gccgttccgg gccagcggca gcgtcacctc
     3601 gatcgcggtg cgcagctccg agcggccgag ggtgtaggcc gcgcgctcca gatcacgatc
     3661 cacggattcg atggccgcgc gcaccgtacg gaccagcagc ggcagcgcca tcacggtggc
     3721 cgcgatcacc gccgcgtacc acgtgaaggc caccgtgaat ccggaaagct cgtagagcgg
     3781 cgcgcccagg acgccgcgcc ggcccagcag cacgatcagg tagtagccca ccacggtggg
     3841 cgggagcacc agcggcagcg tgaccagcag gtcgaccagg ctccggcccc agaagcgacg
     3901 gcgcgccagc acgtaggcga tgggaatgcc caccagcgcg ttcagcgcgg tggccgccac
     3961 cgcgacctgg agcgacagcc ggagcgcgac gagattgtct gggctcatga tgggtcgacg
     4021 cgatgccccg gtagtatgcc atgcccggat gccccgacgg ccggagcagg gccccctggt
     4081 gaagcagagc tgcctagcgc acgcccgccg gcggcggctg gaagccgaaa cggccgagca
     4141 ccgcccggcc ttcgcgactc agcagcagct cgatgaacgc ctgggcaagc gcgggctgcc
     4201 gcgtccccgc cacgacggcg gccgggtacg cgatcgggcg gtacgtgtcc tcggcgggcc
     4261 ggaaggcttc cttcaccgcc tggccgcgga gcgccacgtc ggtggcgtag acgaagcccg
     4321 cgtccacctc gccccgcgcc acgtactcca gcgcctggcg cacgttctcc gcgaagatca
     4381 gcttgggctg cacgcggtcc cagagcccga gggcccggag gctctcctcc gcgtactggc
     4441 ccgcgggcac ggccctggga ttgcccacga cgatccgggc gacccgcgcg cccgccagat
     4501 cggtgggctt ggcgatatcc acccgcgagt cggccggctt gatcacggtg agcaggttcc
     4561 gcgcgaacac ccggcgcgtg ccgggcacga tgagtccgcg cttctcgagc tcgtccatct
     4621 ggacgacggc cgccgagatg aacaggtcga tgggaccgcc cgcctcgatc tgcttctgca
     4681 gctcgccgga ggagccccag ttgtagagca gggtcacgcc gggccgggcc gccgtgaagc
     4741 cgcgcccgat ctcggtgacc gcatccttca tgctgatcgc cgccgacacc gtcagcgact
     4801 gagcatccgc ggcggacgcc aggcccagca ggaccgcgag cgtcacgggg atccagcgct
     4861 tcatggacat gcgtgcctcc cggcccccat cgtaccgccg cccgcctcgg tcaggcgcgc
     4921 cggcgctgct ggatgagact cagcgcccag ttcaccagga aggtgatcag cagcaggatc
     4981 accgacaggg cgatggcgag gtcgaagttg cccttgccgg tctcgagcac ggtggcggtg
     5041 gtgagcaccc gggtggagcc ccggatgttg ccgcccagca tcatggaggc acccacctcc
     5101 gagatcacgc cgccgaagcc cgccatcacc gcagccagca tcggcaggcg cgcttccttg
     5161 agcagcaccc agagcatctg ccctcgcgag gcgccgaggc ccagcatctg cagccggaac
     5221 gtctccggca cctgctggac cgcggccagg gtgagcccgg tgacgatggg cgccgcgatc
     5281 acgaactgtc ccgtcaccat cgccgacggc gtgtagagca gctcgagaaa gccgagcgcg
     5341 ccgttgcgcc agaggaggat cgtcacgaac aggcccacca ccaccggcgg gagccccatg
     5401 ccggtgttca cgaggctgac cacgagagcc cggccgggga agcgcgcgag cgcgagcagc
     5461 gcgcccagcg ggatcccgac cagcagcgag agggcggtcg cgcccgcgga gacctgcagc
     5521 gacagccagg tgatctgcca gacctccgcg tcccccgcca gcagcagccg gaacgcttcg
     5581 agaacgccct gcccgaagag ccccaccgtg gacgccgcgg cggctagagg tcctcgtcct
     5641 tcttgccggc caccgggacg aagagcggct ggccgtactt gtccacgccg aagctcttga
     5701 tcaccgcctg cgtctcgggc gccagcatga agtccgcgaa ggccttgccg cccctggcgt
     5761 tgacgcgcgg gccgttggcc gggttcacct ccatcaccga gtagatgttc agcagtgggc
     5821 gatccttctc gacgaggatc ggcaggtcca cccgcttctg gaacgcgagg aaggtcgccc
     5881 gatcggtcag cgcgtaggcg cgccggtcgt tggcgatgcc gagcgtctgt cccatgccct
     5941 gtccggactc gatgtaccag gcgcccttcg gctcgacgcc ggcctgcttc cacagggcca
     6001 gctcgagggt gtgggtcccc gacttgtcgc cgcgcgagac gaagcgcgac cggctctccg
     6061 cgatgcgccg gagcgcgtcc agcgctctgg gcagcccctt gatcctcgcc ggatcgtcgg
     6121 gtggcccgat gacgacgaag tcgttgtaca tgacgaggcg ccggttggtc atcttgccct
     6181 cgtccatgta cttcttctcg aggccgggcg cgtgggcgag ggtgacgtcg gcctccccgc
     6241 gggccgccag ggccagggcc tggccggtgc cgaccgagat ggccttcacg gtcagcccgg
     6301 tctgcttctc gaacatgggc accagcacgt ccagcagccc ggagtcctgg gtgctggtgg
     6361 tggtggacag gatgacgacg ttcgactggg cgaccgccgg ccccgtcgtg gacgggagca
     6421 ggccggccag cgccagcgac agcacggcag cgagtcgcgt gatcatcgtg cgggttttca
     6481 aggctctatg cgggccggag catacaggct cggtggagca tagtcaacgt ggtagactcg
     6541 ccgccggctc tccgcgactc ggtgtatcat gacgagcgcg cgcccaaccc ccaggagaca
     6601 ccgatgcgac gattcgatct cgttctgccg aacagcctcg aagactgcct gaagatcctc
     6661 gcggagcacg gaggacgggc caagctggtg gcgggaggca ccgacctcct gccccagatg
     6721 aagaacggag ccctgcatcc cgccctggtg gtggacctct ccgggatcgc ccgggtgcgc
     6781 caggtccaga acggcagcgg cctgcgcatc ggcgccgcgg tcaccgcgcg cgagctcgag
     6841 ctgagcccgg cgctgcgcgg gccctatctc gcgatcgcgg agagcgcggc catggtcggc
     6901 tcgctgcagg ttcgcaacct ggccacggtg ggcggcaatc tctgcaatgc cgcgccctcg
     6961 gccgacatgg cgccgccgct gatggccctg gaggcccagg cggtcatcgc ggggccgggc
     7021 ggcgagcggc gcgtgccgat ggccgacttc ttcaccgggg tccgcaagac ggtgctcgcg
     7081 ccgaacgagc tgctggtcga gctgatcgtg ccgacgccgg gggcccacag cggcggccag
     7141 tacctgcggc acacgccgcg gcgcgagctc gatattgccg tcgtgggggt agcttctcag
     7201 ctcacgatgg ccgacggtcg ctgcaccaag gcgcgcatcg ccctggcctc ggtggccccg
     7261 acgccggtcc gcgccaccgc ggccgagcgg tcgctcgagg gccaggcggt gacgcccgag
     7321 gccatcgagc gcgcggccac actggcgatc gaagcggccc ggcccatcga cgaccagcgc
     7381 ggctccatcg agttccgcaa gcacctcgtc cgcgtcctca cccgtcgcac gctcaccacc
     7441 gcgctccagc gcgcgaacgc ctgatccgga ggcagatccc cgtggccaaa caactcctgc
     7501 gctgcaccgt gaacggcgag gccgtcgagg tactcgtcca gccgtacgtc aacctcctcg
     7561 acgccctgcg cgagaacctg gggctcaccg gccccaagga aggctgcggc accggcgact
     7621 gcggcgcctg caccgtccac gtggacggtc gtgtggtggc gagctgcctg atgctggcca
     7681 tgcaggcccg cggccggaag gtccgcacca tcgagggcct agcatccgcg gataccaccc
     7741 tgcacccgct gcaggaggcg ttcgtccggc acggggtacc ccagtgcggc ttctgcattc
     7801 cgggagtgtt gatggcggcg gccgcgctgc tggaggagaa tccgcggccc tccgaggagg
     7861 agatccgcta cggcatcgcc ggaaatctct gccgttgcac cggctacacc gcgatggtga
     7921 ccgcgatcag cgaggcggcg gaaaccctcg cctcgcagag gagcgctcga tgagcacgat
     7981 gaagaccacg cgcgacgtca agggagtggg catctcgatt ccgcggccgg acggccccga
     8041 gaaggtcacg ggccgcgtgc a
//
LOCUS       DASUFA010000129        13093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_947072, whole genome shotgun sequence.
ACCESSION   DASUFA010000129 DASUFA010000000
VERSION     DASUFA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947072"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFX87_04690"
     CDS             <1..252
                     /locus_tag="VFX87_04690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349332.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX4994241.1"
                     /translation="ELSDEDKLIVARARKLQRFLSQPFFVAEVFTGQPGRYVKLADTI
                     KGFKEIVEGKHDELPEQAFYMVGGIEEAQEKAAKMREAK"
     gene            252..665
                     /locus_tag="VFX87_04695"
     CDS             252..665
                     /locus_tag="VFX87_04695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX4994242.1"
                     /translation="MLLELATPTRQLVSEQVDEVVAPGTEGYFGVLPGHAAFLATLGS
                     GEVIYRTGRQEQHIAVHGGFAEVTPERVIILAETAERPDEIDRERAERARQRAEQRMA
                     GRPVEGESDEIDFDEAVAAYRRALTRLLVASIGGG"
     gene            806..1189
                     /locus_tag="VFX87_04700"
     CDS             806..1189
                     /locus_tag="VFX87_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4994243.1"
                     /translation="MTILIADDEPAIVDLVRFTLEDTHVRIVDAADGAVAVELARRER
                     PDVALLDVQMPRLDGLEACRQLRRLPECAHTRIVMLTAAAQAADRRRGLEAGADHYLT
                     KPFSPLALLTLVRSLVPEAPVWPAK"
     gene            1174..1929
                     /locus_tag="VFX87_04705"
     CDS             1174..1929
                     /locus_tag="VFX87_04705"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX4994244.1"
                     /translation="MAGEVGLSQALSYARDLKSLYERSRARERELEQTQDRLRAAYQQ
                     SLQYAIDLRKTYRRLQQSSFHSLLGLANALEAKDGYTRGHSGRVAALARRMALAAGVA
                     PGAADTIAQAGLLHDLGKIGIPESVLRKPGPLSEEEWAMMRRHPIVGAQIVAPLEFFA
                     DGAVIVRHHHERHDGSGYPDGLRGEMIPLGARIVAVADVYDALTSDRPYRPRLARADV
                     VRRLESEAGQTLDARLAALCVHLTDDAAPERSV"
     gene            1904..2728
                     /locus_tag="VFX87_04710"
     CDS             1904..2728
                     /locus_tag="VFX87_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994245.1"
                     /translation="MRLLSAPFEPGALPPPHGAVRAYGQVGGAVSLVAAAGLALEWAG
                     VAPFSLGHALASGAVHGGLLALGWLLATDAAAGWTGPAIRLGATLLLASVTARLHPLG
                     ALAYLLVPGLLLLEAGRQPRLRAMGLGGVRPGHAAAGLAVGTFLGAHLLVTASRTLGY
                     RLQAGGLPAYLSALAYDVGANALSAEWVFRGAVFSLLWRRWTFWPAALVATALSLVRY
                     LLDPALPHAVEARAGAVFYLGLVGLIACALRAWSSSLLPGYLAALAFFAAYRMLAP"
     gene            2725..4251
                     /locus_tag="VFX87_04715"
     CDS             2725..4251
                     /locus_tag="VFX87_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX4994246.1"
                     /translation="MIALPVALLAAMALAVVLADQGPASVLRHLYLVPTLWAAMTWGA
                     IGGGLVGVLAGLLHAPVVLPAVERLGLTSQTLDGLLSLGLPIVAGLLVGRLVDGSGAR
                     AARLAAVLAIQRRLAGPGAMEDKLVSVARELRAALRARRLALLVGADVRTALLASVPM
                     GTLVDPDSAAAFALATGQRVSSADLGEDPRVRAAEPMGPTPVRALAVPLVSGAGVVGV
                     MAVEWSGELSRARRAAAEELALHMGLAVENARLTLRQHHFAEELAEKVSAATARLREL
                     DQAKTEFLSVVSHELRTPLTALQGFSELLLRSALPPERAQRCLVYLHTEACTLGRIVG
                     ELMDLTRIEAGHPLELKPELIDLAGLIERNLEIFAAEHREHRFRWSSSGSEVALRADP
                     DALDRMVKNLLSNAVKYSPRGGQVCVSASPSEDQPGMIELAVEDDGVGIPADQLPRIF
                     DKYVRVPHPETATARGLGLGLSLVKALAEAHGGRVEVDSLPGKGSRFRLLLPVADREF
                     "
     gene            4314..4592
                     /locus_tag="VFX87_04720"
     CDS             4314..4592
                     /locus_tag="VFX87_04720"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX4994247.1"
                     /translation="MARRDEVAGRMAEAVVKRLVGRKLADVRDETRAREVVRRILAEN
                     FQAEEKLDADARTLLMDNAKLIRDSASDYKRLFALVKTKLAKERGFTL"
     gene            4598..4882
                     /locus_tag="VFX87_04725"
     CDS             4598..4882
                     /locus_tag="VFX87_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX4994248.1"
                     /translation="MSRERMFALADRIVADLAATESVMIKGDDEKAHGQLRTEVFRVL
                     EDESKLEESIDQEVRRALSTYSRPAPEGSSEWELLYQKTRDEVYRRRFRL"
     gene            4879..5472
                     /locus_tag="VFX87_04730"
     CDS             4879..5472
                     /locus_tag="VFX87_04730"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX4994249.1"
                     /translation="MKRVVVGLSGASGAIYGIRFLELLRGIPDVEAHLVISDAGRRTI
                     VEETDYTVADVEALATRRYRSRDIGAALASGSFRTDGMVIVPCSIKTAAAVAHCVSDT
                     LMARAADVTLKEGRPLILVVRESPLHLGHLRVLTALAEMGAVVLPPMPAFYNRPKQID
                     DLVVHTLARVLDRLGLPHSLAPEWQGAPPRVPGPPRS"
     gene            5469..6248
                     /locus_tag="VFX87_04735"
     CDS             5469..6248
                     /locus_tag="VFX87_04735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4994250.1"
                     /translation="MTPPAARPELAPAPLDLSIVVPVYNEEENLPLLWPEIREVLEPT
                     GLRYEVIFVDDGSRDRSAEIVRGFREQDRRVRLLRLKANAGETAATDAGLKAVRGRHV
                     VVMDADLQNDPHDIPAMLRQLDRWDAVTGWRLNRAAGDSWVRRLSSRIANRIRNRLSE
                     ETIQDSGCTFRAFRRECLRDLVLYKGFHRFIPTLLKMRGFRVLEVPVNHRPRRFGQSK
                     YGIGNRAVRAFVDLLVVRWMKDRLLRYQIAEDLGGDAGGGR"
     gene            6245..7219
                     /locus_tag="VFX87_04740"
     CDS             6245..7219
                     /locus_tag="VFX87_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4994251.1"
                     /translation="MKVLLVGLGRWGEKHLRVLGELGVELWVADVSAERRAFAIKAGV
                     AAARAVEDFRRALPHVEAVDIVTPADNHLALAGECLRAGRDCFIEKPLALTVDEGRRL
                     AAIVAETGRVLQVGHIFRFHPVTTVLRDRLAAGSLGRVRYCTGRFAGFKRPRTDVGVT
                     QTDAIHYFDLFAYLLGRPATAVTATLRDHLGRGLDDCSFATVDYGDVPAFVEAGYFAP
                     GTYRDCVIVGEQGTLAADFGTSEVRVLANRHVETPSGWQAPEGAVESLKASGPEPLRH
                     ELERFLDAVTRRSRPAVDVEAGLDALRTVEAAQRSSALGRRVTLAELA"
     gene            complement(7272..7358)
                     /locus_tag="VFX87_04745"
     tRNA            complement(7272..7358)
                     /locus_tag="VFX87_04745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7322..7324),aa:Leu,seq:cag)
     gene            7432..7902
                     /gene="smpB"
                     /locus_tag="VFX87_04750"
     CDS             7432..7902
                     /gene="smpB"
                     /locus_tag="VFX87_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX4994252.1"
                     /translation="MADETDTKVISTNRKAFHDYSILETYEAGLVLRGTEVKSLREAQ
                     VNFKDSYATIDNDEAWLVGCYISPYHHGTDANHDPERRRKLLLHKREIARLLGKIAER
                     GLTLIPLRLYFKAGRVKVELGLARGKKLHDKRASLRERESRREMEKESRARSRA"
     gene            7905..8260
                     /gene="ssrA"
                     /locus_tag="VFX87_04755"
     tmRNA           7905..8260
                     /gene="ssrA"
                     /locus_tag="VFX87_04755"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8325..9302)
                     /locus_tag="VFX87_04760"
     CDS             complement(8325..9302)
                     /locus_tag="VFX87_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994253.1"
                     /translation="MIRQRVLRGTLLGAALVIVAAAVGCSRLAIWSAPAKTPSATRTA
                     AAMDADKLFWTTLHAGEYARIPDALTALKAAYLENPRDAVTAAHVGWLHIWRLGERAR
                     QTALRPDITDDAVLARKYFEEAVRLDPGEARYLGFYAGLLMAEGTIHKDEKMVRRGFY
                     AMKDAVRAWPEFNYFTAGYTASALPVESDRYRKALEQQWLNLDVCTGQKVDRANPDYA
                     RFMALETKEGPRRVCWNSWIAPHNFEGFFLNFGDMLVKAGQPDLAVAMYRNARHAREY
                     AAWPYKAVLEARIRDAAKNVEAFRRTEPAPDATPLMVRSTFACMACHQE"
     gene            9380..10033
                     /locus_tag="VFX87_04765"
     CDS             9380..10033
                     /locus_tag="VFX87_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4994254.1"
                     /translation="MSVKRRRRKKPSPRHARSGAAGGGLRDTLVDAAVALIARKGPQG
                     FSLREVARRARVSEAAPYWHFADREALLAAVAERGFEEMTKEMMEIWSRETEPAERFR
                     ALGIGYVRFALAQPSYLRVMFGSEVPDKAEHPALKAAGERTFALLVQAVTECQAAGQV
                     RGGDPEAFAVAAWSIVHGLAALLIDGKLKDHATTTAEAERLAHTITDCFMLGLAPRA"
     gene            10030..10602
                     /locus_tag="VFX87_04770"
     CDS             10030..10602
                     /locus_tag="VFX87_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994255.1"
                     /translation="MIAKLLLAAAVALLTLTSAPAAFAEKEWAVTLLGGQYSGSKLIE
                     LGGRADFKDSYTAGLSVSYQFVDWGKHIRWELEGQMLKHFGEQEHVEFASSINARWIT
                     FPWNRYLETSVAIGGGLSVTSEVPVLEKRDPDNSNAATLLHYLLLEAEIGLPNSNWSL
                     VGRVHHRSGIFGLFSHSGSNVLAVGVRYRF"
     gene            complement(10599..12965)
                     /locus_tag="VFX87_04775"
     CDS             complement(10599..12965)
                     /locus_tag="VFX87_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4994256.1"
                     /translation="MVGFASDMGEFGIGQSVKRFEDVRLLRGEGRFHADVNLAGQVHA
                     VLVRSPHAHARIRAIDTAPALRAPGVLAVYTGADVARDGLGTMRMTLKRSRPDGSPMF
                     APPHRGLAMDRARHVGDAVAMVVAETRAQAEDAADLVRVDYEPLPSVTDTAAAAEPGA
                     PAVWDECPDNISNLYPAGDAAATDAAFARAHRVVRRRYVITRVHAQFMEARGALGVYD
                     PGEDRYTLYADVQYPHRVRNALATNIFKIPEHQIRVIAGDVGGAFGTKGWQYVEHRLV
                     LWAARKLGRPVKWLCERREAIPADEHARDNVTDAELALDAQGRFLALRVRTLANVGAY
                     VSSDRNLLATFSNVATLVGVYTFPAAHVEVRCMLTNTNSTAPYRGAGRPEATYVIERL
                     IDDAARELGEDRLALRRTNLIPAAAMPYKTALGVTYDCGEFEKSMDAALRLSDVAGFA
                     ARQEASKARGRLRGLAVVNAIEQAAGPQPEFAEIRFAPSGSATVFMGTKNQGQGHETT
                     FKQVLHERLGLDPADVRYIDGDTDRVGFGMGTMGSRSTVIGGTALWMAADKVIAKGRK
                     IAARLLEAAEADIGFGDGKFSVVGTDRALSLKEVARAAFQPAQLPAGLEPGFYETGTF
                     APKQATWPNGCHVCEVEVDPETGDVILARYAIADDVGTVINPLTLKGQIHGGVAQGVG
                     QALMEQVVYEPGSGQLLTASFMEYAMPRADTFCDMVIESHPVPTKLNPLGAKGAGEAG
                     TVGALPAVINAVMDALAPLGVRQLDMPASSDRVWQAIQRARAAGARPA"
BASE COUNT         1759 a         4579 c         4763 g         1992 t
ORIGIN      
        1 gagctctccg acgaggacaa gctgatcgtg gcccgggccc gcaagctgca gcgcttcctg
       61 tcgcagccgt tcttcgtggc cgaggtgttc accggccagc cgggccgcta cgtcaagctc
      121 gccgacacca tcaagggctt caaggagatc gtcgagggca agcacgacga gctgcccgag
      181 caggcgttct acatggtcgg cggcatcgag gaagcgcagg agaaggcggc gaagatgcgc
      241 gaggccaagt agtgctgctg gagctcgcca cgccgacccg ccagctcgtc tccgagcagg
      301 tcgacgaggt ggtggcgccg ggcaccgagg gctacttcgg ggtgttgccc ggccacgccg
      361 ctttcctcgc cacgctgggg agcggcgagg tcatctaccg gaccggccgc caggagcagc
      421 acatcgcggt gcacggcggc ttcgccgagg tcacgcccga gcgggtgatc atcctggccg
      481 agaccgccga gcgccccgac gagatcgacc gcgagcgcgc cgagcgggcg cgccagcgcg
      541 cggagcagcg gatggcgggg cggccggtgg aaggcgagtc cgacgagatc gacttcgacg
      601 aggcggtggc cgcgtaccgg cgcgccctca cgcggctgct ggtcgcttcg atcggcggcg
      661 gctagcccgc tgcccttcgg gcggggcaag ggcacccgca ccctgccccg cgctccgggc
      721 actctccgtt ctctcctgat cccaagccgc tgattccacg cggcgtcccc cgtggcacgc
      781 ccctcgcact cgaggagtgg ccatgatgac catcctgatc gccgacgatg aacccgcaat
      841 tgtcgacctc gtccgcttca cgctcgaaga cacgcacgtg cggatcgtcg acgccgccga
      901 cggggccgtt gcggttgagc tggcccggcg cgagcgcccc gacgtggccc tgctcgacgt
      961 gcagatgccg cgcctcgacg ggctggaggc ctgccggcag ctccggcggc tgcccgagtg
     1021 cgcccatacc cggatcgtca tgctcaccgc ggcggcgcag gcggccgacc gccgccgcgg
     1081 gctcgaggcg ggcgccgacc actacctgac caagcccttc tcgccgctgg ccctgctgac
     1141 gctggtccgc tcgctcgtgc ccgaggcccc ggtatggccg gcgaagtagg tctctcccag
     1201 gccctgtcct acgcgcgcga tctgaagtcg ctctacgagc gctcgcgggc gcgggagcgc
     1261 gagctcgagc agacgcagga ccgcctgcgt gccgcgtacc agcagtcgct gcagtacgcg
     1321 atcgatctcc gcaagacgta ccggcgcctg cagcagtcga gcttccacag cctgctcggc
     1381 ttggccaacg cgctcgaggc caaggacggt tacacgcgcg ggcactcggg gcgcgtcgcg
     1441 gcgctggcgc ggcggatggc gctcgcggcg ggcgtcgcgc cgggggccgc cgacaccatc
     1501 gcgcaggcgg ggctcctgca cgatctggga aagatcggga tcccggagag cgtgctgcgc
     1561 aagcccggcc cgctcagcga ggaggagtgg gccatgatgc gccggcatcc gatcgtcggc
     1621 gcgcagatcg tcgcgccgct cgagttcttc gcggacggcg ccgtgatcgt gcgtcaccat
     1681 cacgagcggc acgacggcag cggatacccc gacgggctgc gcggcgagat gattcccctg
     1741 ggcgcgcgca tcgtggcggt ggccgacgtc tacgacgcgc tcacctcgga ccgcccctat
     1801 cgcccgcggc tggcccgggc cgacgtggtg cgccgcctcg aatcggaagc gggccagacg
     1861 ctcgatgccc ggctggcggc tctctgtgtc cacctcaccg acgatgcggc tcctgagcgc
     1921 tccgtttgag cccggcgcgc tgccgccgcc gcacggggcg gtccgcgcct acgggcaggt
     1981 ggggggcgcc gtctcgctgg tcgcggcggc gggcctcgca ctggagtggg ccggggtggc
     2041 tccgttctcg ctcggtcacg ccctggccag cggggccgtc cacggcgggc tgctcgcgct
     2101 gggctggctg ctcgcgaccg acgcggccgc cggctggacc gggccggcca tccggctcgg
     2161 cgccacgctg ctcctggcct cggtgaccgc gcgcctgcac ccgctggggg cgctcgcata
     2221 cctgctggtg cccggattgc tgctgctcga agcggggcgg cagccgcgcc tgcgggccat
     2281 ggggctggga ggcgtccgac cgggccacgc ggcggcgggc ctggcggtcg ggacgttcct
     2341 gggcgcgcat ctgctcgtca ccgcctcgcg aaccctcggc tatcgcctgc aggccggcgg
     2401 cctccccgcc tatctctcgg cgctcgcgta cgacgtgggc gccaacgcgc tcagcgcgga
     2461 gtgggtcttc cggggcgcgg tgttctcgct gctctggcgg cgctggacgt tctggcccgc
     2521 cgcgctcgtc gcgacggcac tgtcgctcgt gcgctacctg ctcgatcccg cgctgccgca
     2581 cgcggtggag gctcgggcgg gggcggtgtt ctacctgggc ctggtcgggc tcattgcctg
     2641 cgcgctgcgc gcctggagca gcagcctcct gcccgggtat ctggcggcgc tcgcgttctt
     2701 cgccgcctac cggatgctcg cgccgtgatc gcgctaccgg tcgccctgct ggccgcgatg
     2761 gcgctggccg tggttctggc ggaccagggc cccgcgtcgg tgctcaggca cctctatctg
     2821 gtgccgacgc tgtgggccgc gatgacctgg ggcgccatcg gcggcggcct ggtcggcgta
     2881 ctggccggtc tgctgcacgc gcccgtggtc ctgccggcgg tggagcgcct gggcctcacc
     2941 tcgcagacgc tcgacggcct gctgtcgctc ggtctgccga tcgtggcggg cctgctcgtc
     3001 ggtcggctgg tagacggctc gggcgcgcgc gcggcgcggc tggccgcggt gctcgcgatc
     3061 cagcgccggc tggccggacc cggcgcgatg gaggacaagc tcgtgagcgt ggcccgcgag
     3121 ctgcgtgccg cgctgcgggc ccggcggctc gcgctgctgg tcggcgccga cgtgcgcacc
     3181 gcgctcctcg ccagcgtgcc gatgggcacg ctcgtcgatc cggactcggc ggcggccttc
     3241 gccctcgcca cgggccagcg cgtgagcagc gcggatctgg gggaggatcc ccgcgtgcgg
     3301 gcggccgagc caatgggccc gacgcccgtg cgcgccctcg ccgtcccgct cgtctcgggc
     3361 gcgggcgtcg tcggggtgat ggcggtcgag tggagcggcg agctgtcgcg cgcgcgccgc
     3421 gccgcggccg aggagctggc gctgcacatg ggcctggcgg tagagaacgc gcgcctcacc
     3481 ctccgccagc accactttgc cgaggagctc gccgagaagg tgtcggcggc caccgcgcgg
     3541 ctccgcgagc tggaccaggc gaagaccgag ttcctctcgg tggtctcgca cgagctgcgc
     3601 acgccgctga ccgcgctgca aggcttcagc gagctgctgc tgcgatcggc gctgcctccg
     3661 gagcgcgccc agcgttgcct cgtctatctc cacaccgagg cgtgcacgct gggccgcatc
     3721 gtcggggagc tgatggacct gacccggatc gaggccgggc acccgctcga gctcaagccg
     3781 gagttgatcg acctcgccgg gctgatcgag cgcaacctgg agatcttcgc ggccgagcat
     3841 cgcgagcacc gttttcgctg gagctcgagc ggatccgagg tggcgctgcg cgcggatccc
     3901 gacgcgctgg accggatggt caagaatctg ctctccaacg cggtcaagta ctcaccgcgc
     3961 ggcggccagg tctgcgtctc cgcgagtccg tccgaggatc agccggggat gatcgagctg
     4021 gccgtcgagg acgacggtgt cggcatcccg gccgatcagc tgccccggat cttcgacaag
     4081 tacgtccgcg tgccccatcc cgagaccgcc acggcccgcg gcctggggct gggtctgagc
     4141 ctggtcaagg cgctcgcgga ggcccacggt ggacgggtcg aggtggacag cctgcccgga
     4201 aaagggtccc gatttcgcct gcttctcccg gtggccgacc gagagttttg atccatattt
     4261 ccgtatagtt gttgacactc acgttcgtgc ccgtgtaccc tgtctctccg ggcatggcgc
     4321 gacgggacga ggtagccggg cggatggcgg aggccgtggt gaagcggctg gtcgggcgca
     4381 agctcgccga cgtccgggat gagacgcgcg cgcgggaggt ggtccgacgg atcctggccg
     4441 agaacttcca ggccgaggag aagctcgacg ccgacgcgcg caccctcctg atggacaacg
     4501 ccaagttgat ccgagactcc gcctccgact acaagcgcct cttcgcgctc gtcaagacga
     4561 agctggccaa ggaacggggg ttcacgctgt gacgcggatg agccgcgagc gcatgttcgc
     4621 gctggccgac cggatcgtgg cggatctggc cgcgaccgaa agcgtcatga tcaagggcga
     4681 cgacgagaag gcccacggcc agctccgcac cgaggtgttc cgggtcctgg aggacgagtc
     4741 gaagctcgag gagtcgatcg atcaggaagt gcggcgcgcg ctgagcacct attcgcggcc
     4801 ggcgcccgag ggcagctcgg agtgggagct gctctaccag aagacccgcg acgaagtcta
     4861 ccggcgccgg ttccggctat gaagcgcgtg gtcgtcggcc tgagcggcgc ctcgggcgcg
     4921 atctacggca tccgcttcct ggagctgctg cgcggcatcc cggacgtcga ggcgcacctc
     4981 gtcatctcgg acgcggggcg gcgcaccatc gtcgaggaga ccgactacac ggtcgccgac
     5041 gtcgaggcac tcgccacccg ccgctaccgc agccgggaca tcggcgcggc gctcgcctcc
     5101 ggctcgttca ggacggacgg catggtcatc gtgccgtgca gcatcaagac cgccgccgcg
     5161 gtggcccact gcgtctccga caccctgatg gcgcgcgcgg ccgatgtgac cctcaaggag
     5221 ggccggccgc tcatcctggt ggtccgcgag agcccactgc atctcgggca cctccgagtg
     5281 ctcaccgcac tggccgagat gggcgcggtg gtcttgcccc cgatgcccgc gttttacaac
     5341 cggccgaagc agatcgacga cctcgtcgtc cacacgctgg cccgcgtgct cgaccggctc
     5401 ggcctgccgc attcgctggc gccggagtgg cagggcgccc cgccgcgggt gccggggccg
     5461 ccccgcagtt gacccctccc gccgctcgtc cggagctcgc gcccgccccg ctggatctct
     5521 ccatcgtggt gccggtgtac aacgaggagg agaacctccc gctgctctgg ccggagatcc
     5581 gcgaggtcct cgagccgacc ggcctgcgct acgaggtcat cttcgtggac gacgggagtc
     5641 gtgaccgcag cgccgagatc gtccgcgggt tccgcgagca ggaccggcgc gtgcggctcc
     5701 tgcgcctgaa ggcgaatgca ggggagaccg cggccaccga cgcgggcctg aaggcggtgc
     5761 gcggccgaca cgtggtcgtc atggatgccg atcttcagaa cgacccgcac gatattcccg
     5821 cgatgctccg gcagctcgac cggtgggacg ccgtgaccgg ctggcgcctg aaccgggccg
     5881 cgggcgattc gtgggtgcgg cggctctcct cccgcattgc caaccggata cgcaaccggc
     5941 tgagcgagga gactatccag gacagcggct gcaccttccg tgcgttccgc cgcgagtgcc
     6001 tgcgagacct tgtgctctac aagggcttcc accgcttcat tccgacgctg ctgaagatgc
     6061 ggggcttccg ggtcctcgag gtgccggtga atcaccggcc gcgccggttc ggccagtcca
     6121 agtacgggat cggcaaccgc gcggtccgag ccttcgtgga tctcctcgtg gtgcgctgga
     6181 tgaaggaccg cctcctgcgc taccagatcg ccgaggatct gggcggcgac gcggggggcg
     6241 gccggtgaag gtgctgctgg tcgggctggg gcgctggggg gagaagcatc tgcgcgtgct
     6301 cggcgagctg ggcgtcgagc tctgggtggc cgacgtctcg gcggagcggc gcgccttcgc
     6361 gatcaaggcc ggcgtggcgg cggcgcgcgc ggtcgaggac ttccgccggg cgctgccgca
     6421 cgtagaggcg gtggacatcg tgaccccggc cgacaatcat ctcgccctgg ccggggagtg
     6481 cctgcgcgcg ggccgcgatt gcttcatcga gaagccgctc gccctcacgg tggacgaggg
     6541 ccgtcgcctc gccgcgatcg tggcggagac ggggcgcgtg ctgcaggtcg ggcacatctt
     6601 ccgcttccat cccgtgacga cggtgctgcg cgaccggctg gccgcgggct cgctcgggcg
     6661 cgtgcgctac tgcacgggcc gcttcgccgg cttcaagcgc ccgcgcacgg acgtgggcgt
     6721 gacgcagacg gacgccatcc actacttcga cctcttcgcc tacctgctcg ggcgcccggc
     6781 gaccgcggtc accgccaccc tgcgcgatca cctgggccgc ggtctcgacg actgctcgtt
     6841 cgccaccgtg gactacggcg acgtccccgc cttcgtggag gccggctatt tcgcgccggg
     6901 gacctatcgc gactgcgtga tcgtgggcga gcaggggacg ctcgccgccg acttcggcac
     6961 ctccgaggtg cgggtgctcg ccaatcgcca cgtggagacg ccgtccgggt ggcaggcgcc
     7021 ggagggcgcg gtagaaagcc tcaaggcttc cggtcccgag ccgctgcgcc acgagctcga
     7081 gcggttcctg gacgcggtga cccgccggag ccgtcccgcg gtggacgtgg aggcgggtct
     7141 cgatgcgctc cgtacggtgg aggcggcgca gcgctcctcc gcgctcggcc gtcgcgtcac
     7201 cctcgccgag ctggcgtagt cgcgccgtac gctcgggacg atcgatatga ggattcgggc
     7261 ggggtttcta ctggtgccga aggggggact cgaaccccca cacccttgcg ggcacatgac
     7321 cctgaatcat gcgcgtctgc caattccgcc acttcggctc gggcaaggcc tccatcttac
     7381 ggaccgcgcg gttgaagtca aggggcccgc ggccttcccg gcctgagcgc catggcggac
     7441 gagacggaca cgaaggtcat ctccaccaat cgcaaggcct tccacgacta ctccattctc
     7501 gagacgtacg aggcggggct cgtgctgcgg ggaaccgagg tcaagtcgct ccgcgaggcg
     7561 caggtcaact tcaaggacag ctatgcgacg atcgacaacg acgaggcgtg gctcgtcggc
     7621 tgctacatca gcccgtatca ccacggcacc gacgccaatc acgatccgga gcggcggcgc
     7681 aagctgctgc tgcacaagcg ggagatcgcg cgcctgctcg ggaagatcgc cgagcgcggg
     7741 ttgaccctca tcccgcttcg tctatacttc aaggcagggc gggtgaaggt cgaactgggg
     7801 ctggcccgtg gcaagaagct ccacgacaag cgcgcgtccc tccgcgagcg cgaaagccgc
     7861 cgggagatgg aaaaggaatc acgcgcccga tctcgggcgt agaagggggc gactggtttc
     7921 gacggggatc agtgacgcac aggtggcatc acgaggtgct ctctcctcgt aaaactgggg
     7981 gcacggttat agctgccgac aaccagctag ctctggcggc ctaacacccg ccagcgtcct
     8041 ccggattggg ttaccagggt ccgggtcagg gcgtcatatt ctggtgactg gctccaaacc
     8101 ctgctccggg gtgcggggcg agatctacag gagctggctg atcggtgacc cggccggttg
     8161 gggggccgag aggcgagacg caggagataa ccggctaatg atgtagatgc ctgttgccga
     8221 gggtttccgg acgtgggttc gatgcccacc gcctccacca attcgtagca accgcccgcc
     8281 gggccgtaac gaggcccggc gggcggtccc ctttcactcc cggctcactc ctggtggcag
     8341 gccatgcagg cgaaggtcga gcggaccatg aggggggtcg cgtccggcgc gggctcggtc
     8401 cggcggaacg cctccacgtt cttcgcggcg tcacggatgc gggcttccag cacggccttg
     8461 tagggccagg ccgcgtactc ccgcgcgtgg cgggcgttgc ggtacatcgc caccgcgagg
     8521 tccggctggc ccgccttcac cagcatgtcg ccgaagttga ggaagaaccc ctcgaagttg
     8581 tgcggggcga tccaggagtt ccagcacacg cgccggggtc cctccttcgt ctccagcgcc
     8641 atgaagcgcg cgtagtccgg gttggcccga tccaccttct gccccgtgca cacgtcgaga
     8701 ttcagccact gctgctcgag cgctttccgg taccggtcgg actcgacggg cagggcggag
     8761 gcggtgtagc ccgccgtgaa gtagttgaac tccggccatg cccgcaccgc gtccttcatg
     8821 gcgtagaacc ctcgccgcac catcttctcg tccttgtgga tggtgccctc ggccatcagc
     8881 agcccggcgt agaagcccag gtaccgcgcc tcgccgggat cgagccggac cgcctcctcg
     8941 aagtacttgc gggcgagcac cgcgtcgtcc gtgatgtcgg ggcggagcgc ggtctgccgg
     9001 gcccgttcgc cgagccgcca gatgtgcagc cagccgacgt gggcggcggt gaccgcgtcg
     9061 cgcgggttct cgaggtaggc ggccttcagc gcggtgaggg cgtccgggat gcgggcgtac
     9121 tcgccggcgt ggagcgtcgt ccagaagagc ttgtcggcgt ccatcgcggc ggcggtccgt
     9181 gtcgcgctcg gcgtcttggc cggcgccgac cagatcgcaa gccggctgca tccgacggcg
     9241 gccgcgacga tcaccagggc cgcgccgagg agcgttcccc gcaacacgcg ctgtcggatc
     9301 atggtaccct cccgcgcggg gtttgggcat gccgccccgc cgccccgaat gacgagccga
     9361 acgttaacgc tgttaagtga tgtctgtcaa gcgccgccgc cggaagaagc cgtccccccg
     9421 ccacgccagg tccggcgcgg ccggcggggg cctgcgcgac acgctggtgg acgcggcggt
     9481 ggccctgatc gcgcgcaagg ggccccaggg cttcagcctg cgggaagtgg cccggcgcgc
     9541 gcgggtgtcg gaggccgcgc cctactggca cttcgcggat cgcgaggcgc tgctggccgc
     9601 ggtggcggag cgcggcttcg aggagatgac caaggagatg atggagatct ggtcgcggga
     9661 gaccgagccg gcggagcgct tccgggcgct cggcatcggg tacgtgcgct tcgcgctggc
     9721 gcagccgtcg tacctgcggg tgatgttcgg ctccgaggtg ccggacaagg cggagcatcc
     9781 cgccctgaag gcagcgggtg agcgcacgtt cgcgctgctc gtccaggcgg tcaccgagtg
     9841 ccaggcggcc gggcaggtgc gcggcggcga ccccgaggcg tttgcggtgg ccgcctggtc
     9901 catcgtccac ggcctggccg cgctgctcat cgacggcaag ctgaaggacc acgcgaccac
     9961 caccgcggag gccgagcggt tggcgcacac gatcaccgat tgtttcatgc tcggcctcgc
    10021 gccccgcgca tgatcgccaa gcttctcctc gccgccgcgg ttgcgctcct gacgctgacc
    10081 tccgcgcccg ccgccttcgc ggagaaggaa tgggcggtga ccctgctggg cggtcagtac
    10141 tcggggagca agctcatcga gctgggcggg agggcggact tcaaggactc gtacaccgcc
    10201 gggctctccg tctcctacca gttcgtggac tggggcaagc acattcgctg ggagctggaa
    10261 gggcagatgc tcaagcactt cggagagcag gagcacgtgg agttcgcgtc ctccatcaat
    10321 gcgcgctgga tcacgtttcc gtggaaccgc tatctcgaga cctcggtggc catcggcggg
    10381 ggactctcgg tgacgagcga ggtgccggtg ctcgagaagc gggatcccga caacagcaac
    10441 gccgcgaccc tgctgcacta cctcctgctc gaggccgaga tcgggctacc gaactcgaac
    10501 tggagcctcg tgggccgcgt ccaccaccgc tcgggcatct tcggcctctt ctctcacagc
    10561 ggctccaacg tgctggcggt cggcgtgcgc taccgcttct aggccgggcg cgcgcccgcc
    10621 gcccgcgcgc gctggatcgc ctgccacacg cggtcgctgc tcgcgggcat gtcgagctgg
    10681 cgcaccccga gcggggccag cgcgtccatc accgcgttga tgacggcggg cagcgcgccg
    10741 accgtgccgg cctctcccgc gcccttggcg ccgagcgggt tgagcttggt gggcaccggg
    10801 tggctctcga tgaccatgtc gcagaaggta tcggcccgag gcatcgcgta ctccatgaag
    10861 gacgcggtca ggagctggcc ggacccgggc tcgtagacga cctgctccat gagcgcctgg
    10921 cccaccccct gggcgacccc gccgtggatc tggcccttca gcgtcagcgg gttgatgacg
    10981 gtccccacgt cgtcggcgat cgcgtagcgt gccaggatca cgtcgccggt ctccgggtcc
    11041 acctcgacct cgcagacgtg gcagccgttg ggccacgtgg cctgcttggg cgcgaacgtg
    11101 ccggtttcgt agaagccggg ctcgaggccg gccgggagct gggccggctg gaaggcggcg
    11161 cgcgccactt ctttcaatga gagggcccgg tccgtgccca ccaccgagaa cttcccgtcg
    11221 ccgaatccga tgtccgcctc ggcggcttcc agcagccgcg ccgcgatctt gcgccccttg
    11281 gcgatgacct tgtcggcggc catccacagc gcggtgccgc cgatcacggt ggagcgggag
    11341 cccatggtgc ccatgccgaa gccgacgcga tcggtgtcgc cgtcgatgta gcgcacgtcg
    11401 gcggggtcga ggccgagccg ctcgtggagg acctgcttga aggtggtctc gtgcccctgg
    11461 ccctggttct tggtgcccat gaacacggtg gcgctgccgc tcggcgcgaa gcggatctcc
    11521 gcgaactcgg gctgcggtcc cgccgcttgc tcgatggcgt tcaccacggc cagcccccgc
    11581 agccggccgc gcgccttcga tgcctcctgc cgcgccgcga agccggcgac gtccgagagc
    11641 cggagggcgg cgtccatgga cttctcgaac tcgccgcagt cgtaggtgac gccgagcgcg
    11701 gtcttgtagg gcatcgcggc cgccgggatg aggttcgtgc gccgcagggc caggcggtcc
    11761 tcgccgagct cgcgggccgc gtcgtcgatg agccgctcga tgacgtaggt ggcctcgggc
    11821 cgccccgcgc cccggtacgg cgcggtggag ttggtgttgg tcagcatgca gcggacctcg
    11881 acgtgggcgg ccgggaacgt gtagacgccc acgagggtcg ccacgttgct gaaggtggcg
    11941 agcagattgc gatcggacga gacgtaggcg cccacgttgg cgagggtgcg cacgcgcagg
    12001 gcgaggaagc gcccctgggc gtcgagcgcc agctcggcgt cggtcacgtt gtcgcgcgcg
    12061 tgctcgtcgg ccgggatcgc ctcccgccgc tcgcagagcc acttcaccgg ccggccgagc
    12121 ttgcgcgcgg cccacagcac gagccggtgc tcgacgtact gccagccctt ggtcccgaag
    12181 gcgccgccca cgtcgcccgc gatgacgcgg atctgatgct ccgggatctt gaagatgttg
    12241 gtcgccagcg cgttgcgcac gcggtgcggg tactgcacgt cggcgtagag cgtgtagcga
    12301 tcctcgcccg ggtcgtagac gccgagggcg ccgcgcgctt ccatgaactg cgcgtgcacg
    12361 cgggtgatca cgtagcggcg ccggaccaca cggtgggcgc gcgcgaaggc ggcgtcggtg
    12421 gccgccgcgt cgcccgccgg gtagagattg gagatgttgt ccgggcactc gtcccagacc
    12481 gcgggcgcgc cgggctcggc ggccgccgcg gtgtcggtca ccgacgggag cggctcgtag
    12541 tccacgcgca ccaggtcggc ggcgtcctcg gcctgcgcgc gggtctcggc caccaccatc
    12601 gcgaccgcgt cacccacgtg acgcgcccgg tccatcgcga gcccgcggtg cggcggcgcg
    12661 aacatcggcg agccgtcggg ccgggagcgc ttgagggtca tcctcatggt gccgaggccg
    12721 tcgcgcgcca cgtcggcgcc agtgtagacg gcgagcaccc ccggggcccg gagagcgggg
    12781 gcggtgtcga tcgcgcggat gcgcgcgtgg gcgtggggag agcgcaccag caccgcgtgg
    12841 acctggccgg cgaggttcac gtcggcgtga aagcgcccct cgccccgcag cagtcggacg
    12901 tcctcgaatc gtttgacgga ctgcccgatg ccgaactcgc ccatgtcgga ggcgaagcct
    12961 accacaaatt gatccttcgc cacctcctcg ctccgttgct agaatggctt cgctgcgccc
    13021 ccttggatca tgatgcgacg actctcgatg cccgtctggt tggccctggc tctggtgggc
    13081 tgcctcgtcg gct
//
LOCUS       DASUFA010000130         6876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1520077, whole genome shotgun sequence.
ACCESSION   DASUFA010000130 DASUFA010000000
VERSION     DASUFA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520077"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /gene="rimM"
                     /locus_tag="VFX87_04780"
     CDS             <1..400
                     /gene="rimM"
                     /locus_tag="VFX87_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4994257.1"
                     /translation="TLWEPQGDRREPCRIASCRFEGETVYLRMDGVDSPEAARRFCGR
                     LLAVNREEALPLAEGTFYPWQLEGAVVETRDGRAVGRFVGVEPGAQELWLVDDGTRRR
                     LIPAVAEIVVEVSVADRRIVIDPPEGLLDL"
     gene            397..1158
                     /gene="trmD"
                     /locus_tag="VFX87_04785"
     CDS             397..1158
                     /gene="trmD"
                     /locus_tag="VFX87_04785"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX4994258.1"
                     /translation="MRIDIVTLFPGMLEAPLAESILGRARARGLVDIRVHDLRRYATG
                     RHRVTDEPPFGGGGGMILKPEPLAAAIESLRAAGSAEAPARVILLGPAGRHFSQVVAR
                     EMARRPHLVLVCGRYEGVDERVSEQLVDEELSIGDYVLTGGEPAALVVADAVTRLLPG
                     VLGDEDAPARDSFARGLLEHPQYTRPEVFRGAAVPEVLRSGDHGRIARWRTLMSLWRT
                     WQRRPDLLETADLTPEEQKWTDAFRQGRTPADLLD"
     gene            1183..1560
                     /gene="rplS"
                     /locus_tag="VFX87_04790"
     CDS             1183..1560
                     /gene="rplS"
                     /locus_tag="VFX87_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX4994259.1"
                     /translation="MEAIRIVEAGDLKKDRGGFRPGDTVKVMVKVVEGEKERLQAFEG
                     VCIRRRGGGTGASFTVRRISYGVGVERTFPVHSPRIDKIQVMKRATVRRSKLYYLREL
                     VGKAARLKEKRIAQVPTSGEQSS"
     gene            1571..2224
                     /locus_tag="VFX87_04795"
     CDS             1571..2224
                     /locus_tag="VFX87_04795"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX4994260.1"
                     /translation="MAAGAPYRFESAAWRRGITRLAGVDEAGRGPLAGPVVAAAVVLE
                     PGARIAGVDDSKRLPPEERRRLFDVIRERAVGVGVGVVDHVTIDRINILQATRLAMGQ
                     ALGALGIEPELVLTDYVAVPGLHCPQRNLVEGDRRSASVAAASIIAKVTRDRIMEEAD
                     REYPVYGFGRHKGYPTPEHRMALEQHGPCALHRRTFAGIWNQLALFSRSELQWIKAD"
     gene            2239..2619
                     /locus_tag="VFX87_04800"
     CDS             2239..2619
                     /locus_tag="VFX87_04800"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX4994261.1"
                     /translation="MPTDTRKITGVRGEEEAARFLARSGLAILDRNVRTRAGEIDLVA
                     KEGKTLVFVEVKTRRAAEGDPPQAAVNTRKQNRLGKLAHGYLKLKRLRQVSCRFDVVS
                     VIFNDEGAVKAIRHIPNAFSVSAW"
     gene            2714..3364
                     /locus_tag="VFX87_04805"
     CDS             2714..3364
                     /locus_tag="VFX87_04805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX4994262.1"
                     /translation="MHRDGTFRQALPLDPKHTALLVVDMQRAFVEEGQVMEVPPARDV
                     IPRIQELVGIFRERHLPVIFTEFTYSPAAPLLVGQLHPEHRPAGPGAPTGFGLPSSSC
                     LEGEDNARVIPELAPRADELVIRKHYYDGFNGTVLDGALRARGVTTLVITGTMTDICV
                     LGTVIGGFNREYRLIVVEDGVATLWPEIQHTTLDIIRRAYARVLSAKEVADELERG"
     gene            3395..4324
                     /locus_tag="VFX87_04810"
     CDS             3395..4324
                     /locus_tag="VFX87_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX4994263.1"
                     /translation="MTNGLRFRGCMPANLLPFTADLAIDEPAYRKHLRWLADAPGVTG
                     IVANGHAAEVASLTRDERKRTLAIALDEVADKVPIVTGVYCDGTLEAVELARDARAGG
                     AAGLLVFPPTLFMWGAQQKPDMVLRHFQTIADAVDLPLIVFEYPPASGIGYSPETLAE
                     LCKIPTVAGVKDWSNDIVSYEKNLRALRGAGRPVAMLSSFTMSLMASFMLGADGCISG
                     MGSVTADLQASLFAAVQAGRLDEARGINDRLAPLVQVFYAPPFVDMHNRMKEALAILG
                     RIPVAHVRPPLTPVSDDERHRIRLALRAAGLSA"
     gene            4486..5739
                     /locus_tag="VFX87_04815"
     CDS             4486..5739
                     /locus_tag="VFX87_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4994264.1"
                     /translation="MRVLPGGVLGGYYAPQELAFVVREARGPRLYDFSGREYIDYILG
                     SGPLVLGHAHPAVVAAVEAQLRKGTTYFQLSEPTLALAEEICRAVPCAEQIRFCGSGS
                     EATFFALRVARAFRQRDKILKFEGGFHGVHDYSLMSVGPRAPKAFPAPTPDSAGIPHA
                     IQGEVLVAPFNDVAEAQAMIEAHHHDLAAVIMEPLQRLVVPQPGFLQGVRDITARLGV
                     PLIFDEIVTGFRFAYGGAQEYYGVVPDLAAFGKIIGGGFPLAAVAGRRDIMRHFSQEM
                     SEGGFVQQAGTLNGNPIAAAAGLATLAELRRPGTYERLFATGTRLKQGLEAAARRAGL
                     PAQVAGEAPVFEIYFTDRPITDYRATLSADQALHAAFTREMLTRGVVKAAQKFYVSLV
                     HTEVEVARTLEVFGEALTAVAGTSR"
     gene            5775..>6876
                     /locus_tag="VFX87_04820"
     CDS             5775..>6876
                     /locus_tag="VFX87_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994265.1"
                     /translation="MANHDNDAQEFWYRGKITRRRWLGLSATAAGAIGAGMLVPAPWR
                     AAFGQAKPYKLGTLQPLSGTAAAGGKTALVGVQMAVDRINKGGGVNGRPIELIVADYE
                     SKPDVGRRKAEKLVLEDKIDAHAGGYLSNVCLACMPVYEEHKVVNMITVCLDTTITTS
                     KCSRYTFRPFDFAPSQAVAIAPHLVNKVGKKWHIAYADYAWGQSTRDAYAQEIKKLGG
                     EVTGTTGVPLGTADMTAFLSKISGAYDGLFGIFFGPQGISFVNQSYDLGIAKKARIAG
                     DGAIVVATSLPAMGAKADGFVGVDRYIPLLEGKLNTPSHKKFLDESVARLKPLDVSLP
                     DRYVQSNFEGVNFLKLGMERTKFRGREDTTKLI"
BASE COUNT         1053 a         2351 c         2492 g          980 t
ORIGIN      
        1 cacgctgtgg gagccccagg gggaccggcg cgagccgtgc cgcatcgcct cctgccgctt
       61 cgagggagaa accgtctatc ttcggatgga cggcgtggac tcgccggagg cggcccggcg
      121 gttctgcggc cggctgctgg cggtgaaccg ggaggaggcc ctgcctctcg cggaaggcac
      181 cttctatccg tggcagctcg agggggccgt ggtagagacg cgcgacggcc gggcggtggg
      241 ccggttcgtg ggcgtggagc cgggcgcgca ggagctgtgg ctggtggacg acgggacccg
      301 ccgacggctc atccccgcgg tggcggagat cgtggtggag gtcagcgtgg cggatcgccg
      361 gatcgtgatc gatccgccgg aagggctgct ggacctgtga ggatcgacat cgtcaccttg
      421 ttcccgggga tgctggaggc gccgctcgcc gagtccatcc tggggcgggc ccgcgcgcgc
      481 gggctcgtgg acatccgcgt gcacgacctc cggcggtacg cgaccgggcg gcaccgggtg
      541 accgacgagc cgcccttcgg cggcggcggc ggcatgatcc tgaagccgga gcccctcgcc
      601 gcggcgatcg aaagcctgcg ggccgcgggg tcggccgagg cgcccgcgcg agtgatcctg
      661 ctgggcccgg ccgggcgcca cttcagccag gtggtggcgc gggagatggc ccgccggccc
      721 catctggtgc tcgtctgcgg ccgctacgag ggcgtggacg agcgggtgag cgagcagctc
      781 gtggacgagg agctgtccat cggcgactac gtgctgaccg ggggcgagcc ggcggccctg
      841 gtggtggcgg acgcggtcac gcgcctgctg cccggcgtgc tgggcgacga ggacgcgccc
      901 gcgcgcgatt ccttcgcgcg gggactgctc gagcacccgc agtacacgcg gcccgaggtc
      961 tttcgcggcg cggccgtgcc cgaggtgctg cgctcgggcg atcacgggcg catcgcccgc
     1021 tggcgcaccc tgatgtcgct gtggcgaacg tggcagcgcc ggccggacct cctggagacc
     1081 gccgatctca ctccggaaga gcagaagtgg accgacgcgt tccggcaggg ccggacgccg
     1141 gcggatctcc tggactaatc atcgagcaag gagcgtcaga tcatggaagc gatccgaatc
     1201 gtcgaggcgg gtgatctcaa gaaggaccgg ggcggcttcc ggcccgggga caccgtcaag
     1261 gtgatggtga aggtggtgga gggcgagaag gagcggctgc aggcgttcga aggcgtgtgc
     1321 atccgacgcc gcgggggtgg caccggggcc tcattcacgg tgcggcggat ctcctacggc
     1381 gtgggcgtgg agcgcacgtt cccggtgcac tcgccgcgga tcgacaagat ccaggtgatg
     1441 aagcgggcga cggtgcgccg gtccaagctc tactatctcc gcgagctggt cggcaaggcg
     1501 gcgcgcctga aggagaagcg catcgcccag gtgcccacct ccggcgagca gtcgagctaa
     1561 gacgacgacg ttggcggcgg gggcccccta ccgcttcgag agcgccgcgt ggcggcgggg
     1621 catcacccgt ctcgcgggcg tggacgaggc ggggcggggc ccgctggccg ggccggtggt
     1681 cgcggcggcg gtcgtactcg agccgggcgc gcggatcgcg ggggtggacg actcgaagcg
     1741 gctcccgccg gaggagcggc ggcggctctt cgacgtgatc cgcgagcggg cagtgggggt
     1801 gggggtgggg gtcgtggacc acgtgacgat cgaccggatc aacatcctgc aggcgacgcg
     1861 gctggcgatg gggcaggcgc tgggcgcgct ggggatcgag ccggagctgg tgctgacgga
     1921 ctacgtggcg gtgccggggc tgcactgccc gcagcggaac ctggtggaag gcgatcgacg
     1981 gtcggcctcg gtggcggcgg cctccatcat cgccaaggtg acgcgggacc gcatcatgga
     2041 ggaagcggac cgcgagtatc cggtgtacgg attcgggcga cacaaggggt acccgacgcc
     2101 ggagcaccgg atggccctcg agcagcacgg gccgtgcgcg ctacaccgcc gcacgttcgc
     2161 gggaatctgg aaccagctgg cgctgttttc gcgcagcgag cttcaatgga tcaaggctga
     2221 ttaaggagtc gttgccacgt gccgaccgac acccgaaaga tcaccggcgt caggggcgag
     2281 gaagaagcgg cccgcttcct cgcgcggagt gggctcgcca tcctggaccg caacgtgagg
     2341 acccgcgcgg gggagatcga cctcgtggca aaggagggca agaccctcgt cttcgtcgag
     2401 gtcaagaccc gacgcgccgc cgagggcgat ccgccgcagg ccgccgtgaa cacgcgcaag
     2461 cagaaccggc tcggcaagct ggcgcacggc tatctcaagc tcaagcggct gcggcaggtc
     2521 tcctgccggt tcgacgtcgt ctccgtgatc ttcaacgacg agggggcggt caaggccatc
     2581 cgccacatcc cgaatgcgtt ctcggtatcg gcctggtagt aagcggtcgc gtggaaagca
     2641 gcagccgggt gaggcgcagc cgaaggcgag ccgaacggat acaagaaagc ttgaagagct
     2701 aagcgaaagg ccgatgcacc gcgacggaac attccgccaa gccctccccc tggaccccaa
     2761 gcacaccgcc ctgctggtcg tggacatgca gcgcgcgttc gtggaggagg gccaggtgat
     2821 ggaagtccct cccgcccgcg acgtgatccc gcgaatccag gagctggtcg gcatcttccg
     2881 cgagcggcat ctgccggtga tcttcaccga gttcacctat tcaccggcgg cgcccctgct
     2941 ggtgggccag cttcatcccg agcaccggcc cgcgggcccc ggcgccccga ccggcttcgg
     3001 cctgccctcg tcctcgtgcc tggagggcga ggacaacgcg cgggtgatcc ccgagctcgc
     3061 cccgcgcgcg gatgagctgg tgatccgcaa gcactactac gacgggttca acggcacggt
     3121 gctggacggc gcgctccggg cgcgcggcgt caccacgctc gtgatcaccg gcaccatgac
     3181 cgacatctgc gtgctcggca ccgtgatcgg cggcttcaac cgcgagtacc ggctgatcgt
     3241 ggtcgaggac ggcgtggcca cgctgtggcc cgagatccag cacaccacgc tcgacatcat
     3301 ccgccgggcc tacgcccggg tgctctccgc caaggaagtg gcggacgagc tggagcgcgg
     3361 ctgaccgccg cgtgggtatg ctagagttct cgccatgacg aacgggctcc gcttccgcgg
     3421 gtgcatgccg gccaacctcc tgcccttcac cgccgacctg gccatcgacg agccggccta
     3481 ccgcaagcat ctccgctggc tcgccgacgc gcccggcgtc accgggatcg tggccaacgg
     3541 ccacgccgcc gaggtcgcct cgctgacgcg ggacgagcgc aagcggaccc tggcgatcgc
     3601 cctcgacgag gtcgcggaca aggtgcccat cgtcaccggc gtctactgcg acgggaccct
     3661 cgaggcggtg gagctggcgc gcgacgcgcg cgcggggggg gcggccggcc tgctcgtctt
     3721 cccgccgacc ctgttcatgt ggggcgcgca gcagaagccg gacatggtgc tgcgccactt
     3781 ccagaccatc gccgacgcgg tggacctgcc gctcatcgtc ttcgagtacc cgcccgcgtc
     3841 gggcatcggc tacagcccgg agacgctcgc cgagctgtgc aagatcccga cggtggccgg
     3901 ggtgaaggac tggtcgaacg acatcgtgtc gtacgagaag aacctgcgcg cgctgcgcgg
     3961 cgcgggcagg ccggtggcga tgctctcgtc cttcaccatg tcgctgatgg cgagcttcat
     4021 gctgggggcc gacggatgca tctcgggcat gggcagcgtg accgcagacc tgcaggcgtc
     4081 cctcttcgca gcggtacagg ccggacggct cgacgaggcg cgggggatca acgaccggct
     4141 ggcgccgctc gttcaggtgt tctacgcgcc gccgttcgtg gacatgcaca accgcatgaa
     4201 ggaagccctc gcgatcctcg gccgtatccc ggtcgcccac gtgcggccgc cgctgacgcc
     4261 cgtctccgac gacgagcggc accggatccg cctggccctc cgggcggccg ggctctcggc
     4321 ctaggtcgcc ggcccctcga gtccctccgg cggccgagta agcgcaccct tgcggccggg
     4381 gacgctttca gggtataaaa ccgtccaatc tccgcatccc agcgaggtgg aatggcaggt
     4441 ccggctggcc gcagtcgcga agaattggct ctgctcgaga cggcgatgcg ggtcctcccg
     4501 ggaggcgtcc tgggggggta ctacgccccg caggagctgg ccttcgtcgt gcgcgaggct
     4561 cgggggccgc ggctctacga cttctcgggc cgcgagtaca ttgactacat cctgggtagc
     4621 ggccccctcg tgctgggcca tgctcatccc gcggtggtcg ccgcggtcga ggcccagctc
     4681 cgcaagggta cgacctactt ccagctcagc gagcccaccc tggccttggc cgaggagatc
     4741 tgccgggccg tcccgtgcgc cgagcagatc cggttctgcg ggagcggcag cgaagcgacc
     4801 ttcttcgcgc tccgcgtggc gcgcgccttc cgccagcgcg acaagatcct gaagttcgag
     4861 ggcggcttcc acggcgtcca cgactactcc ctgatgagcg tgggcccgcg ggcgcccaag
     4921 gcgttccccg cccccacgcc ggactcggcg ggcattcccc acgccatcca gggcgaggtc
     4981 ctggtcgcgc ccttcaacga cgtggccgag gcccaggcca tgatcgaggc gcatcaccac
     5041 gatctggccg cggtgatcat ggagccgctg cagcggctgg tggtgccgca gcccgggttc
     5101 ctccagggcg tccgggacat caccgcgcga ctcggcgtgc cgctgatctt cgacgagatc
     5161 gtcaccggct ttcgcttcgc ctacgggggc gcccaggagt actacggggt cgtgccggac
     5221 ctggccgcct tcggcaagat catcggcggc ggctttccgc tcgccgcggt cgccggccgc
     5281 cgggacatca tgcggcactt ctcgcaggag atgagcgagg gcggcttcgt ccagcaggcg
     5341 ggcacgctca acggcaaccc gatcgccgcc gcggcgggcc tcgccaccct ggccgagctg
     5401 cgccggcccg ggacctacga gcgcctcttc gccaccggca cgcgcctcaa gcaggggctc
     5461 gaggcggccg cgcgccgggc gggcctgcct gcccaggtag cgggcgaggc acccgtgttc
     5521 gagatctact tcaccgaccg gcctatcacc gactaccggg ccacgctgtc cgcagatcag
     5581 gccctccacg ccgccttcac gcgcgagatg ctcacccgcg gcgtggtcaa ggcggcccag
     5641 aagttctacg tgtcgctcgt ccacaccgag gtggaggtgg cgcgcacgct ggaggtcttc
     5701 ggcgaggcgc tcaccgcggt ggcgggaact tctcgctgag cacgccgatt acgcagtcac
     5761 aggaggctct cacaatggca aatcacgaca acgatgcgca ggagttctgg taccgcggca
     5821 agatcacgcg ccggcgctgg ctcgggttga gtgcgaccgc ggcgggggcg atcggggcgg
     5881 gcatgctggt gcccgcgccg tggcgcgcgg ccttcgggca ggccaagccc tacaagctcg
     5941 gcacgctaca gcccctctcg ggcaccgcgg cggccggcgg caagaccgcc ctcgtgggcg
     6001 tgcagatggc ggtagaccgc atcaacaagg ggggcggcgt caacggccgg ccgatcgagc
     6061 tgatcgtggc cgactacgag tccaagccgg acgtcggccg gcgcaaggcc gagaaactgg
     6121 tcctcgagga taagatcgac gcgcacgcgg gcggctatct ctccaacgtc tgcctcgcct
     6181 gcatgccggt ctacgaggag cacaaggtgg tcaacatgat cacggtgtgc ctcgacacca
     6241 cgatcaccac gagcaagtgc agccgctata cgttcaggcc cttcgacttc gcgccgtccc
     6301 aggcggtggc gatcgcgccg cacctggtga acaaggtcgg caagaagtgg cacatcgcct
     6361 acgcggacta cgcctggggg cagtccaccc gcgacgccta cgcccaggag atcaagaagc
     6421 tcggcggcga ggtcaccggc accaccggcg tccccctcgg caccgcggac atgaccgcgt
     6481 tcctcagcaa gatcagcggc gcctacgacg gcctcttcgg gatcttcttc gggccgcagg
     6541 gcatctcctt cgtcaaccag agctacgacc tgggcatcgc caagaaggcg cggatcgccg
     6601 gcgacggcgc catcgtggtg gcgaccagcc tgcccgccat gggggccaag gccgacggct
     6661 tcgtcggtgt cgaccgctac atcccgctcc tggagggcaa gctcaacacc ccctcccaca
     6721 agaagttcct cgacgagtcg gtggcgcgcc tgaagccgct cgacgtctcc ctgcccgacc
     6781 gctatgtgca atccaacttc gagggggtca acttcctcaa gctcggcatg gagcggacga
     6841 agttccgcgg gcgcgaggac accacgaagc tgatcg
//
LOCUS       DASUFA010000131         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1161334, whole genome shotgun sequence.
ACCESSION   DASUFA010000131 DASUFA010000000
VERSION     DASUFA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161334"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFX87_04825"
     CDS             <1..437
                     /locus_tag="VFX87_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4994266.1"
                     /translation="LLGAEWPGNVRQLENCIEQAVVLSERDTIDVDVLPLAEAAGKRG
                     AEGGKPGLPVGLTLRDLEQQYILQTLDGVGGNRTQAARQLGISLRCLQYKLKAYRHAE
                     ALTRSDGSAAEGSRIAPPGSSRRVSSLNDVLSLGDRRRMLMS"
     gene            504..1802
                     /locus_tag="VFX87_04830"
     CDS             504..1802
                     /locus_tag="VFX87_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994267.1"
                     /translation="MTGVPPAEPHESDDGESFARRHVRHNVVALGIDLGLFLVGLSFV
                     SQSTILPAFAAHLGASNLVIGAIPALMTLGWNLPSLFAAGYTESLARKLPFVLRYTIW
                     ERLPFLGLAAVAFFLARPAPGLSLALMLGMLLLITGVGGVLMPAWMDIVARAVPVGLR
                     GRFFAVSSLLGGAGGLLGSVFTVWVLARMPAPDGYAVCFLMAALCVGLSYAALARVRE
                     PRATAVEVAPPLGAYLRRAGRLLREDRNLRWFLLSRALIYVGVMASGFYTVYGLRHYA
                     APDWAVGVFTTALLAGQMTGNVVLGALADRAGHLAALGIGAGALLLANVGALLVPSLE
                     LFIVVFALQGVHLAAVNVSGLSVLLEFAPSPAARPTYVGLGTTLLTPVAFGGPLIAGV
                     MADVLGFPAVFVVAALGSLAGLALLLGRVREPRHRHAGAA"
     gene            1885..2769
                     /locus_tag="VFX87_04835"
     CDS             1885..2769
                     /locus_tag="VFX87_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4994268.1"
                     /translation="MALIFYFPLLLLMVAASDAAAQTPSREQIARGKYVFGAAAGCGC
                     HTVPKQPLNAGGRKYDGPFGTVYSSNITPDPTTGIGKWTDEQIITATRLGRRPNGERL
                     IPVHPYTVFNGMAEQDLKDVVAYLRSVPPVNRATPAKKISVPLFESVFLPAWLATFAA
                     VETPPKSAATSGVARGEYLARAVSHCGECHTPRTMTMAVDNTRFLAGNPKGKGPEGSA
                     VPNITPDRETGLGSWSEEQITDYLETGNKPDGDVAGGLMMEVIQGSSAGYKDLTKADR
                     QAIAKYLKSIPAVKNRIE"
     gene            2835..3326
                     /locus_tag="VFX87_04840"
     CDS             2835..3326
                     /locus_tag="VFX87_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4994269.1"
                     /translation="MNGRLRLTASVIVTASLVIGVAAGCATRSASKMGSGDVVADRQR
                     LMKSVGANWADIQAKAKAGNIEGIAVNAENLALTSQHIPALFPAGSGTEKSKAKPEIW
                     QKWPEFEAAAKNLGMQAEKLRDASRAKNEQLTQDLVKDFGRLGCGTCHTPFRQPPPAQ
                     PRS"
     gene            3479..>3741
                     /locus_tag="VFX87_04845"
     CDS             3479..>3741
                     /locus_tag="VFX87_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4994270.1"
                     /translation="MSTSSRRLGHEAIGAGNGEEAVQKALKDPPDLIILDVMMPIMDG
                     FAVCRTLRERDATRLVPVVVMTALQGVENRVKLLQHGADDVLT"
BASE COUNT          529 a         1296 c         1336 g          580 t
ORIGIN      
        1 tgctgctggg ggcggagtgg ccgggcaacg tgcggcagct ggagaactgc atcgagcagg
       61 cggtggtatt aagcgagcgt gatacgatcg atgttgacgt gctgccgctc gcggaggcgg
      121 cgggcaagcg cggggcagag ggaggcaagc cggggttgcc ggtggggctg acgctgcggg
      181 acctggagca gcagtacatt ctccagacgc tcgacggtgt ggggggcaat cgcacgcagg
      241 cggcgcggca gctcgggatc agcctgcgct gcctgcagta caagctgaag gcctaccggc
      301 atgcggaagc gctcacgcgg tcggacggga gcgcggccga ggggtcgcgc atcgcgcccc
      361 cgggctcgtc gcgccgcgtg tcctcgctca acgacgtgct ctcgctcggc gatcgccggc
      421 gcatgctgat gtcctgagcg gcggcccttc ggggccgccc gcggcttgcc gcgccgctct
      481 caccggggta tcctcggcgg gcggtgaccg gagtccctcc cgctgaaccc cacgagtccg
      541 acgacggcga atccttcgcg cgccgccacg tccgccacaa tgtcgtggcc ctgggtatcg
      601 atctcggtct cttcctcgtc gggctgtcgt tcgtgtcgca gagcacgatc ctgcccgcgt
      661 tcgccgccca cctgggcgcc tcgaatctcg tcatcggcgc gatcccggcc ctgatgacgc
      721 tcggatggaa cctcccgtcg ctgttcgcgg cgggctacac cgaatcgctc gcccggaagc
      781 tgcccttcgt gctccgctac acgatttggg agcggctgcc gttcctcggc ctggccgcgg
      841 tcgcgttctt cctggcccgc ccggcgcccg ggctctcgct cgctctcatg ctcggcatgc
      901 tgctcctgat caccggcgtg ggcggcgtcc tcatgccggc ctggatggac atcgtcgccc
      961 gtgcggtgcc ggtggggctg cgcggacgct tcttcgccgt ctcgagcctg ctgggcggcg
     1021 cgggcgggct gctgggtagc gtgttcaccg tgtgggtgct cgcgcggatg ccggcgcccg
     1081 acggctacgc ggtgtgcttc ctcatggcgg cgctgtgcgt gggactgtcc tacgcggcgc
     1141 tcgcgcgcgt gcgcgagccg cgggccaccg cggtggaggt cgccccgccg ctcggcgcct
     1201 acctgcggcg agccggccgg ttgctgcggg aggatcggaa cctgcgctgg ttcctcctct
     1261 cccgcgcgct gatctacgtc ggcgtgatgg cgtccggctt ctacaccgtg tacgggctcc
     1321 gccactacgc ggcgccggac tgggcggtag gcgtgttcac caccgcgctg ctcgccgggc
     1381 agatgaccgg caacgtggtg ctgggggcgc tcgccgatcg cgcgggccat ctggcggcgc
     1441 tgggcatcgg ggcgggggcg ctcctcctcg ccaatgtcgg cgcgctgctc gtgccgtcgc
     1501 tcgagctgtt catcgtcgtg ttcgccctgc agggcgtcca cctggccgcg gtgaacgtct
     1561 cgggcctgag cgtgctgctc gagttcgcgc cgagcccggc cgcgcggccc acctacgtgg
     1621 ggctcgggac caccctgctg acaccggtgg ccttcggcgg ccccttgatt gccggcgtca
     1681 tggccgacgt gctcgggttc cccgcggtgt tcgtggtggc ggcccttggg agcctggccg
     1741 ggctcgccct gctgctgggc cgcgtgcgcg agccccggca tcgccacgcc ggcgcggcct
     1801 gagcggcccg ggcgcggccg ccgtgatatc ttcgccgggc gggaacctgg gggtccaatc
     1861 gagggttctg aggcataacg tgagatggcg ctcatattct attttccgct cctgctgctg
     1921 atggtcgccg cctcggacgc ggccgcccag accccttcga gggagcagat cgcccgcggc
     1981 aagtacgtat tcggcgcggc ggcgggctgc gggtgccaca ccgtaccgaa gcagccgctg
     2041 aacgccggcg gccgcaagta cgacgggccg ttcgggacgg tctactcgtc aaacatcacg
     2101 cccgacccca ccaccgggat cggcaagtgg acggatgagc agatcatcac cgcgacgcgg
     2161 ctgggccgcc gtccgaacgg cgagcggctc atcccggtcc atccgtacac cgtgttcaac
     2221 ggcatggccg agcaggatct gaaggacgtg gtcgcctacc tgcgctcggt gcccccggtc
     2281 aaccgggcga cgccggccaa gaagatctcg gtcccgctgt tcgagagcgt gttcctcccg
     2341 gcctggctgg ccacgttcgc ggcggtggag acgccaccca agtcggcggc gacctcgggc
     2401 gtcgcgcgcg gcgaatacct ggcgcgcgcg gtgtcccact gcggcgagtg ccacaccccc
     2461 cggaccatga cgatggcggt ggacaacacc cggttcctgg ccgggaaccc gaagggcaag
     2521 ggcccggagg gctccgcggt gccgaacatc acgccggatc gcgagacggg gctcgggagc
     2581 tggagcgagg agcaaatcac cgactacctc gaaacgggga acaagcccga cggagacgtt
     2641 gcgggtgggc tgatgatgga ggtcatccag ggctcctcgg cgggctacaa ggacctgacg
     2701 aaggccgatc ggcaggcgat cgccaagtac ctgaaatcca tcccggccgt caagaacagg
     2761 atcgagtagg accaaccttc accagccgga cgggacagcg cgggagcgcg gcccggtccg
     2821 ccacaggagg cacgatgaac ggacgactga gactcaccgc cagcgtgatc gtcaccgcga
     2881 gcttggtcat cggagtcgca gccggatgcg ccacccgatc cgcatcgaag atgggcagcg
     2941 gcgacgtggt cgcggatcgc cagcgcctga tgaagtcggt cggcgccaac tgggcggaca
     3001 tccaggccaa ggcgaaggcc ggcaacatcg agggcatcgc ggtgaacgcg gagaacctcg
     3061 ccctcacctc ccagcacatt ccggccctct tcccggccgg ctccggcacc gagaagtcga
     3121 aggcgaagcc ggaaatctgg cagaagtggc ccgagttcga ggccgcggcc aagaacctcg
     3181 ggatgcaggc cgagaagctt cgcgacgcct cccgggccaa gaacgagcag ctcacgcagg
     3241 acctcgtgaa ggatttcggc cgtctgggtt gcgggacctg ccacactccg ttccgccagc
     3301 ccccgccggc gcagccgcgc tcgtaacgct cgggtccgcc acatccatcg gggccggcgt
     3361 cacccgcttc cagggtgggc cggcccccgt cgtttgtctg ctagactcgc cgatagacag
     3421 atggcgcgtc cgttcaaggt gctcgtcgtc gacgacgagc cggaaatcgt ccagtacgtt
     3481 gagcacgtcc tcccgccggc tcggtcacga ggcgatcggc gccggcaacg gcgaggaggc
     3541 cgtccagaag gccctcaagg acccgccgga cctgatcatc ctcgacgtga tgatgccgat
     3601 catggacggc ttcgcggtct gccggaccct gcgggagcgc gacgcgaccc ggctcgtccc
     3661 ggtggtcgtc atgaccgcgc tgcagggtgt ggagaatcgt gtgaagctcc tgcagcacgg
     3721 cgccgacgac gtgctcacca a
//
LOCUS       DASUFA010000132         6064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_718771, whole genome shotgun sequence.
ACCESSION   DASUFA010000132 DASUFA010000000
VERSION     DASUFA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718771"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFX87_04850"
     CDS             <1..422
                     /locus_tag="VFX87_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994271.1"
                     /translation="ALVGEEAPFHVLLAREGWHLDLAPFRYLSHFITPPSNPIRFTAR
                     FFLAPVPAGQEPRLLGGEASEGFWIDPAEGHRRFLAGEMPMAEPADCGLGYIAGFDTL
                     GDVWAAHTDGRHKFHGILDRVETAGFRIPRSKRVGQS"
     gene            701..1483
                     /locus_tag="VFX87_04855"
     CDS             701..1483
                     /locus_tag="VFX87_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4994272.1"
                     /translation="MPPSSGPACPIARLLHAGLPSETFRSARLCVSAGERLPAELYTA
                     WRERFGVEILDGIGATETIFMVLSNRPGQSRPGSSGEPVPGTEVRILDAEGRPVPAGQ
                     EGVLWVRTPSQAAGYWQRLDHSRRTFVGDWFRTGDVYRREADGGHVHCGREDDFFKVA
                     GQWVAPAEVESVLLRHPGVLDGGVVGAEEADGLIKTFVFVVPRDPAVEPEALRADLRR
                     FLEGALPPHQRPREIRIVSELPRTATGKLQRFRLRELAHDGR"
     gene            1470..1901
                     /locus_tag="VFX87_04860"
     CDS             1470..1901
                     /locus_tag="VFX87_04860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4994273.1"
                     /translation="MMVANALDVKVRWSEADPAGIVFYPRFFEWFDLGTEALFEALGL
                     PWRELFPAERIVGVPIVETGARFASPVRYGDDVRIETTVSEVREKTFRVEHEISVGGR
                     RCAIGFEVRAWVAQPESPGGALAARPIPAGVVTRLKGESRP"
     gene            1898..2734
                     /locus_tag="VFX87_04865"
     CDS             1898..2734
                     /locus_tag="VFX87_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX4994274.1"
                     /translation="MSPLAGAFLTVHTPRYCTLEAAIEGKLASEVFRPVRDGLVAQGD
                     VVAQAKLDVIVINSCHLITTFPTVVDGTPRHQGVLTAQEAPELIHGVAYDYPGDYDLA
                     ARLIARGRGAGLQCVLANDVHYPLDYGTLMPMVCYLDRAQGTPIVPVSVCLAADLNEA
                     HAWGRQVARVAGESGKRVGFVASGSVSHKLVRGPEKAPLPADQELDHRFARLLADGEF
                     EKLWAWLPEFTRSTEAEMGGRHLAMLLGAVMESGRRFVPTVHAYGPSSGSGNYVISLT
                     GL"
     gene            2770..3984
                     /locus_tag="VFX87_04870"
     CDS             2770..3984
                     /locus_tag="VFX87_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994275.1"
                     /translation="MDKVTRRKFLGTASLAAAGTAMGPWVLRASAQSGPIKIGVVLPY
                     TGVYAVLGESITQAMELVFARENWTVAGRKIEMIKEDDEMKPPVGVRKTEKLIDSDKV
                     DILTGPVHSGILMGMRDKVHNSKTILIVSNAGADAISRDRCSRWIFRTSFSNWQPCQP
                     MGGWVAKNVSKDVFLTAPNYQAGKDMMNAFKETFVPAGGKVVAEDYPKLGETDYAPYL
                     TKIRQSGAKAVFAFFSGSDAVNFVKQYAQYGLKDTIKLTGAGFLTEPDVLPAQGAAAL
                     GVLTGHFYTPLLDNATNHRFVKEFREKYFGKMPDGFACQGHDTAEVIVRALKAVNGNT
                     QDKDKLVDAIAKVEFDSPRGRFKFDPKTHNVIQPFIYVREVREVYGGLNNVVIDRVAN
                     VADPGTGCTLPA"
     gene            4007..4873
                     /locus_tag="VFX87_04875"
     CDS             4007..4873
                     /locus_tag="VFX87_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994276.1"
                     /translation="MPADLFAQALNGLAYGVLLFLLSVGLTLIFGMLDVVNLAHGSFY
                     MLGAYAGLSLIAGTGNFWLALVLAPLAVGLIGALVEVSCLRPLYRRPPLDQVLLTFGF
                     IYLFEDLVKWIWGGRIRSIPPPDLFSGSVSVWGAVIPSYRLFVIVFGLAMAVLLWLLI
                     ERTRLGAVIRAGVFDAEMTGGLGINIRRVFTAVFAFGAALGGLSGVIAGPIQSAYPSM
                     GVAILIPALIVVVVGGLGSLKGSLVGSLIIGQAETFGKAWLPDAAMLIIYVVMATVML
                     LRPQGLFGRPLK"
     gene            4870..5793
                     /locus_tag="VFX87_04880"
     CDS             4870..5793
                     /locus_tag="VFX87_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994277.1"
                     /translation="MTARLLGFAAVVVLAASFPLAAGNYPVKLLQEILIWGIFAMSLD
                     LLMGYAGMVSFGHSAFFGIGGYVAALALAKSPGLVSALVLPALAAGLAALVIGFFSIR
                     VSGVYFIMLTLAFSQMFYAYAFQVSWLGAEDGIVGIPRPAVPGVDLTNIRSFHGYLLT
                     LFVFVGVALWRVVRSPFGHVLRGIHENEARMEAVGYAVDRYKLAAFVIAGVLAGFAGS
                     LYAQFNGSITPDAFFWTTSGEALLMVIIGGTGTLGGPVLGAAAFILLQSLVSTYTERW
                     MLILGGTFILFVLFAPGGIVGALRGRVGLRA"
     gene            5790..>6064
                     /locus_tag="VFX87_04885"
     CDS             5790..>6064
                     /locus_tag="VFX87_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4994278.1"
                     /translation="MTAPLLATDALSRAFGALSALNRVSLEVAPRERRAIIGPNGAGT
                     TTLFNLITGQLAPTAGRILLDGAPIGGLAPHTVARRGVTRSFQRNHL"
BASE COUNT          815 a         2172 c         2096 g          981 t
ORIGIN      
        1 acgcgctggt cggcgaggag gcgcccttcc acgtgctgct ggcccgcgag ggctggcacc
       61 tcgacctggc gcccttccgt tatctctcgc acttcatcac gccgccctcg aacccgatcc
      121 gtttcaccgc gcgcttcttc ctggccccgg tgccggccgg gcaggagccc cgcctgctcg
      181 gcggagaggc ctccgagggc ttctggatcg acccggccga aggccaccgc cgcttcctcg
      241 cgggcgagat gccgatggcc gagccggccg actgcgggct cgggtacatc gcgggcttcg
      301 acaccctggg cgacgtgtgg gccgcgcaca cggatggacg ccacaagttc cacggcatcc
      361 tcgaccgcgt cgaaacggcc ggcttccgca ttcctcgcag caagcgagtc ggccagagct
      421 gagggccggc gcgcgatgga caatgccgcg gccctcttcg tggacgcgcc agtcgaggaa
      481 ggccacggcg cgcgcgcggc gctcctcacg ccgtccggcc ccgtcaccta cgccgcgctg
      541 cagagcctga ccgatcgggc cgcccacgcg ctccgggcgc gcggcgtgga gcatgagcag
      601 cgggtggccc tgctgctccc ggacggcccc gcctgggccg cgaccttctt cgctgcgctc
      661 aagctgggtg cggtggcggt cccgctgaac acgcgcctcg atgccgccca gctccggacc
      721 ggcctgcccg atcgcgcgcc tgctccatgc cggcctgccc agcgagacct tccggtcggc
      781 gcgcctctgc gtctcggccg gcgagcggct gcccgccgag ctctacaccg cctggcggga
      841 gcggttcggg gtggagatcc tcgacggcat cggcgccacc gagaccatct tcatggtgct
      901 ttccaaccgg cccggccaga gccgccccgg gtcgtcgggc gagccggtgc cgggcacgga
      961 ggtcaggatc ctggacgcgg aaggccggcc ggtaccggcc ggccaggagg gcgtgctctg
     1021 ggtccgcacc ccctcgcagg ccgcgggcta ctggcagcgg ctcgaccact cgcgccggac
     1081 cttcgtgggt gactggttcc ggaccggcga cgtctaccgg cgggaggcgg acggcggcca
     1141 cgtccactgc ggccgcgagg acgacttctt caaggtcgcg gggcaatggg tcgccccggc
     1201 cgaggtagaa tcggtcctgc tccgccaccc cggcgtgctc gacggcgggg tcgtcggggc
     1261 cgaggaggcc gacggactca tcaagacctt cgtgttcgtc gtgcctcgcg atccggccgt
     1321 ggagccggaa gcgctgcgcg cggacttgcg gcggttcctg gagggcgcgc tgccgcccca
     1381 ccagcggccg cgggagatca ggatcgtgag cgagctgccg cgcaccgcga ccgggaagct
     1441 ccagcgcttc cggctgcgcg agctggcgca tgatggtcgc taacgcgctg gacgtgaagg
     1501 tgcgctggag cgaggccgac ccggccggca tcgtattcta cccgcgcttc ttcgagtggt
     1561 tcgacctcgg caccgaggcc ctcttcgagg cgctcgggct gccgtggcgc gagctgttcc
     1621 cggcggagcg catcgtgggc gtgcccatcg tggagacggg cgcgcgcttc gcctctccgg
     1681 tgcgctacgg cgacgacgtg cggatcgaga ccacggtgag cgaggtgcgc gagaagacgt
     1741 tccgggtcga gcacgagata tccgtcggcg gtcggcgctg cgccatcggc ttcgaggtgc
     1801 gcgcctgggt ggcgcagccc gagagccccg gcggcgcgct ggcggcccgg cccattccgg
     1861 ccggcgtcgt cacccgcctc aaaggagagt cccgtccatg agcccactcg ccggagcttt
     1921 cctgaccgtc cacaccccgc gctactgcac gctcgaggcg gccatcgagg gcaagcttgc
     1981 ctccgaggtc ttccgcccgg tgcgcgacgg gctcgtcgcg cagggtgacg tggtggcgca
     2041 ggcgaagctc gacgtgatcg tgatcaactc ctgccacctc atcaccacct tccccaccgt
     2101 ggtggacggt accccgcggc accagggcgt gctcaccgcg caggaggcgc ccgagctcat
     2161 ccacggggtg gcctacgact acccgggcga ctacgacctg gccgcgcgcc tcatcgcccg
     2221 cggcagaggg gcgggcctgc agtgcgtgct ggccaacgac gtccactatc cgctcgacta
     2281 cggtacgctg atgccgatgg tctgctacct cgatcgggcg cagggcaccc cgatcgtgcc
     2341 ggtgtcggtc tgcctcgccg cggacctgaa cgaggcccac gcgtggggcc gccaggtggc
     2401 ccgggtggcg ggcgagagcg gcaagcgggt gggcttcgtg gccagcggca gcgtctccca
     2461 caagctggtg cggggaccgg agaaggcgcc cctgccggcc gatcaggagc tcgaccaccg
     2521 cttcgcccgg ctcctggccg acggcgagtt cgagaagctc tgggcctggc tccccgagtt
     2581 cacgcgcagc accgaggccg agatgggcgg ccgtcacctg gccatgctgc tgggggcggt
     2641 gatggagtcg ggccggcgct tcgtgcccac cgtccacgcc tacggcccct cctcgggcag
     2701 cggcaactac gtgatctcct taaccggtct ataatctacc gctcgcactc tcccaaggag
     2761 gggacaggaa tggacaaggt cacacggcgg aagttcctcg gtaccgcatc gctcgcggcg
     2821 gccggcacgg cgatggggcc gtgggtgctg cgcgccagcg cgcaatcggg cccgatcaag
     2881 atcggcgtcg tgctgcctta cacgggggtg tacgccgtgc tgggcgagag catcacgcag
     2941 gccatggagc tggtcttcgc ccgcgagaac tggacggtgg ctgggcgcaa gatcgagatg
     3001 atcaaggaag acgacgagat gaagccgccg gtcggcgtcc gcaagaccga gaagctgatc
     3061 gactccgaca aggtggacat cctcaccggg ccggtgcaca gcggcatcct catgggcatg
     3121 cgcgacaagg tccacaacag caagaccatc ctgatcgtct ccaacgcggg tgccgacgcc
     3181 atcagccgcg accgctgctc gaggtggatc ttccgcacct cgttctcgaa ctggcagccg
     3241 tgccagccga tgggcggctg ggtagcgaag aacgtgtcga aggacgtgtt cctgaccgcg
     3301 cccaactatc aggccggcaa ggacatgatg aacgccttca aggagacctt cgtcccggcc
     3361 ggcggcaagg tggtggccga ggactacccg aagctgggcg agaccgacta cgcgccctac
     3421 ctgaccaaga tccggcagtc cggcgccaag gcggtcttcg ccttcttctc cggctcggac
     3481 gcggtgaact tcgtcaagca gtacgcgcag tacgggctca aggacaccat caagctcacc
     3541 ggcgcgggct tcctcaccga gcccgatgtc ctgcccgccc agggcgcggc ggcgctgggc
     3601 gtcctcaccg ggcacttcta cacgcccctg ctggacaacg ccacgaacca ccgattcgtg
     3661 aaggagttcc gcgagaagta cttcggcaag atgcccgacg ggttcgcgtg ccaggggcac
     3721 gacaccgccg aggtgatcgt ccgggcgctc aaggcggtca acggcaatac ccaggacaag
     3781 gacaagctcg tcgacgccat cgccaaggtg gagttcgaca gcccgcgagg ccgcttcaag
     3841 ttcgacccca agacgcacaa cgtgatccag ccattcatct acgtccgcga ggtgcgcgag
     3901 gtctacggcg ggctcaacaa cgtcgtcatc gaccgcgtcg cgaacgtggc cgatcccggc
     3961 accggctgca cgctcccggc ctagtcgaga ggacgcggca ccccgcatgc cggccgatct
     4021 gttcgcgcag gcgctgaacg ggctggccta cggggtgctg ctttttctgt tgtccgtcgg
     4081 gctcaccctg atcttcggca tgctcgacgt ggtcaacctc gcccacggct ccttctacat
     4141 gctgggcgcc tacgcggggt tgagcctcat cgcgggcacc ggcaacttct ggctggccct
     4201 ggtgctggcc cccctggcgg tgggcctcat cggggcgctg gtggaggtga gctgtctccg
     4261 cccgctctac cggcgcccgc cgctcgacca ggtcctgctc accttcgggt tcatctatct
     4321 cttcgaggac ctggtgaagt ggatctgggg cggccggatc cgctcgatcc cgccgccgga
     4381 tctcttctcc ggctcggtgt cggtctgggg cgcggtcatt ccgtcctacc ggctcttcgt
     4441 gatcgtgttc gggctcgcga tggcggtgct gctctggctc ctgatcgagc gcacccgcct
     4501 gggcgcggtc atccgcgcgg gcgtcttcga cgccgagatg acgggcggcc tcggcatcaa
     4561 tatccgccgg gtgttcaccg cggtcttcgc cttcggcgcg gcgctcggcg ggctctccgg
     4621 cgtgatcgcg gggccgatcc agtccgcgta cccgtccatg ggcgtggcca tcctgattcc
     4681 cgcgctgatc gtggtggtgg tgggcggcct cggcagcctc aagggctcgc tggtcggcag
     4741 cctgatcatc ggccaggccg agacgttcgg gaaggcgtgg ctacccgacg cggccatgct
     4801 gatcatctac gtggtgatgg ccaccgtgat gctgctccgg ccccaggggc tcttcggccg
     4861 cccactcaag tgacggcccg gctcctcggc ttcgccgcgg tcgtggtgct ggccgcctcc
     4921 ttcccgctcg ccgcgggcaa ctacccggtg aagctgctcc aggagatcct gatctggggc
     4981 atcttcgcga tgagcctcga cctgctgatg ggctacgcgg gcatggtgtc cttcggccac
     5041 tccgcattct tcggcatcgg gggctacgtg gccgccctcg cgctcgcgaa gagcccgggg
     5101 ctggtctcgg ccctcgtgct gcccgcgctc gcggccggcc tggccgccct ggtcatcggc
     5161 ttcttctcca tccgcgtgag cggtgtctac ttcatcatgc tgacgctggc cttctcgcag
     5221 atgttctacg cctacgcctt ccaggtgtcg tggctcgggg ccgaggacgg gatcgtgggg
     5281 attccgcggc ccgcggtgcc cggcgtcgat ctgaccaaca tccggagctt ccacggctac
     5341 ctgctgaccc tattcgtgtt cgtcggggtg gccctgtggc gcgtggtgcg ctccccgttc
     5401 ggccacgtcc tgcgcggcat ccacgagaac gaggcgcgga tggaggcggt gggctacgcg
     5461 gtggaccgct acaagctggc ggctttcgtc atcgcgggcg tcctcgcggg gttcgcgggc
     5521 tcgctctacg cccagttcaa cggctcgatc acgccggacg ccttcttctg gaccacgtcg
     5581 ggcgaggcgc tgctgatggt catcatcggc ggcaccggga ccctcggcgg cccggtgctg
     5641 ggcgcggccg ccttcatcct gctgcagagc ctggtcagca cctataccga gcgctggatg
     5701 ctgatcctgg gcgggacctt tatcctcttc gtgctcttcg cccccggcgg gatcgtgggc
     5761 gcgctgcgcg gccgggtggg cctgcgcgca tgaccgcgcc gctcctggcc accgacgcgc
     5821 tgagccgggc cttcggggcg ctcagcgcgc tcaaccgggt gtcgctcgag gtcgccccgc
     5881 gcgagcgccg cgccatcatc ggacccaacg gcgcgggcac gaccacgctc ttcaatctca
     5941 tcaccggcca gctcgccccg accgcgggcc gcatcctcct cgacggcgcg ccgatcggcg
     6001 ggctggcccc gcacacggtg gcccggcgcg gtgtcacgcg ctccttccag cgcaaccacc
     6061 tctt
//
LOCUS       DASUFA010000133         6605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_948580, whole genome shotgun sequence.
ACCESSION   DASUFA010000133 DASUFA010000000
VERSION     DASUFA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948580"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1000
                     /locus_tag="VFX87_04890"
     CDS             8..1000
                     /locus_tag="VFX87_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4994279.1"
                     /translation="MRHTRLGRTGLSVSRLCLGTMTFGLQCDEAASRAILDRAAAGGI
                     TFLDTADVYPVGGGLETVGRTEEIVGRWLEGRRHDFVVATKCVGAMSARPWDRGASRK
                     HILDAIDGSLRRLRTDYVDLYQLHHWDSQTPIDESLRALDDVVRAGKARYVGCSNFHA
                     YQVARALGRSEALGLARFDSVQPRYNLLFRQVERELLPLCREEGIGVIPYNPLAGGFL
                     SGKHHRDTGPTAGTRFTLGNAGERYQDRYWHEREFETVEALRPLAAEAGMPLARLAVA
                     WMLAEPAITSPIVGASRPDQLDDVLPATEKPLDAALKARLDEMTREYRWGDDPR"
     gene            1017..2036
                     /locus_tag="VFX87_04895"
     CDS             1017..2036
                     /locus_tag="VFX87_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019496725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX4994280.1"
                     /translation="MAASVNRQIVLRSRPVGMPRPADFDLVESPIPAPKDGEILSRTI
                     YLSLDPYMRGRISGARSYAQSVEPGQVIVGGTVGLVLESKHPGLAKGDIVLGYDGWQS
                     HAVAKGAAVRKLDPRQAPVSTALGVLGMPGMTAYVGLLDIGQPRPGETVVVSAASGAV
                     GSAVGQIARIKGARAVGIAGSQDKCDYVVRELGFDACVSYKKGDLLASLKEACPNGID
                     VYFENVGGDVLRAVMHLLNMNARIPLCGLISEYNATESVPGPNLRPLLFNRALVKGFI
                     VSDHMARLPEFLKDCGGWLREGRLKHREDIVAGLEKAPEAFIGLLQGKNFGKLLVRVA
                     EDPTR"
     gene            2033..3406
                     /locus_tag="VFX87_04900"
     CDS             2033..3406
                     /locus_tag="VFX87_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994281.1"
                     /translation="MSRRGRLAVAAALLALGLGPAVCSAQVFIASKPNPEFWIAPVLI
                     TANVKRKDVDDRPGPLMIQVSFSVAPPPARDPAEFAQDIFLLWPGELVGTDGVDGADP
                     LLLRQVEAAGFKVLVHGRVPFSARDRRQMGTGAGAAGRRDLGSAFFVTFARPEGLARG
                     AKPATYVRIPWKPEVASLDWIPRLELAAKGAITPRRVSWLEEAFWGRRNIITLSFGDV
                     GYSSLYPFYYGNRDRVIPLAADFSRLMINFAEANHLKIDEVIPSTALRRMSETRENTD
                     TFSIPLIAADGIVPQLLKVQFVYFQGRLPWRPILLSALLLGLGNLTGPIVGNLLRKLI
                     RTLRDRVHVGRGEVTGRARGEVPSQETLARIRPGETTYQDVIRVVGSVPEEEQRLPSG
                     EIRAVIYRGQRLVPHRGRRFGWFATVRHWELENHEVQIDFEQDRVRDIQARIRRTRQT
                     PSTSAAV"
     gene            complement(3427..4218)
                     /locus_tag="VFX87_04905"
     CDS             complement(3427..4218)
                     /locus_tag="VFX87_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994282.1"
                     /translation="MRPRLVAVVLLLAATLPVSAARAADDEVRTIPARPGVTESFLLL
                     RPAGPPVASVIIFAGGDGNLALSAGGIGQLQGNFLVRTRMRWAREGFLVAVLDQPSDR
                     KSGLWNFRTTPEHATDVKQAIAALREIARAPVWLVGTSMGTLSAANAAARLAEGGPDG
                     IVLTSSVTETSKMSYEAVRHAGLGDIRVPTLVVHHKGDTCRSSPYSGAEDILKALKRA
                     PVKELMAFEGGSPPISAPCEAKAPHGYLGLEAQVVSAIGAWIRAH"
     gene            4306..6201
                     /locus_tag="VFX87_04910"
     CDS             4306..6201
                     /locus_tag="VFX87_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEX4994283.1"
                     /translation="MATIYDEVYRRAHDDPEGFWAARAEDIHWEKRWDRVLDDSRPPY
                     YRWFAGGVLNTCYNALDLHVDRGRGKQTALIYDSPVTRTIRGFTYFELRDEVARFAGA
                     LRRQGVAKGDRVIIYMPAVPEAVIAMLACARIGAIHSVVFGGFAPKELATRIDDAKPN
                     VMLSASCGIEGNRVIPYKPLLDQAIAMASHKPSRCLILQRPMETASLVPGRDVDWGEA
                     LAAAGHVECVPVAATDPLYILYTSGTTGIPKGVVRDNGGHAVALKWSMDAVYGMGAGE
                     TFWAASDIGWVVGHSYIVYAPLLKGCTTILYEGKPVGTPDPGAFWRLCAQHGVSAMFT
                     APTAFRAIKKEDPQGAHIGKYDLSRFRTLFLAGERCDPDTLLWAREKLQVPVVDHWWQ
                     TETGWPMAANCVGLGMLPVKPGSPTRVVPGYDIRVLGEDNREMPAGEIGSIAVKLPMP
                     PGSLPTLWHNDEGFERSYLTRHPGYYLTGDAGYKDEDEYIYIMSRVDDIINVAGHRLS
                     TGAMEEVLSAHPDVAECAVMGVADEIKGEVPVGLVVIKAGVARADADLCRELVELVRE
                     KIGPVAAFKTVAVVKRLPKTRSGKILRGTMKKIADGVEHAVPATIDDPAILGEISDSL
                     KALGYPK"
     gene            complement(6234..>6605)
                     /locus_tag="VFX87_04915"
     CDS             complement(6234..>6605)
                     /locus_tag="VFX87_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX4994284.1"
                     /translation="KLVFCLRRLPHLSREEFQRYWLDTHGPLVRELAPALGIKRYTQV
                     HTVTSAFSEAMRRHRGAPEDFDGVAELWWETVEEFARAGATPAGRAAGRRLLEDEKRF
                     IDLARSPIWFGEEHTLVDLAR"
BASE COUNT          949 a         2342 c         2379 g          935 t
ORIGIN      
        1 gatctcgatg cgccacacgc gtctcggccg caccggcctg tccgtctcgc gcctctgcct
       61 gggcaccatg accttcggcc tgcagtgcga cgaggcggcg tcccgcgcca ttctcgaccg
      121 cgccgcggcg ggtgggatca ccttcctcga caccgccgac gtgtatccgg tgggcggcgg
      181 cctcgagacg gtgggccgca ccgaggagat cgtcggccgc tggctcgagg gccgccgcca
      241 cgacttcgtg gtggccacca agtgcgtggg cgcgatgagc gcgcggccgt gggaccgcgg
      301 cgcctcgcgc aagcacatcc tggacgccat cgacggttcg ctgcgccggc tccgcaccga
      361 ctacgtggat ctctaccagc tccatcactg ggactcccag acgccgatcg acgagagcct
      421 gcgcgcgctc gacgacgtgg tgcgggccgg caaggcgcgc tacgtcggct gctcgaactt
      481 ccacgcctac caggtggccc gcgcgctcgg ccgcagcgag gcgcttggcc tcgcgcggtt
      541 cgactcggtg cagccgcgct acaacctgct cttccgccag gtcgagcgcg agctcctgcc
      601 gctctgccgg gaggaaggca tcggggtgat cccgtacaac ccgctcgccg gcggcttcct
      661 ctcgggcaag caccaccggg acaccggtcc caccgcgggc acccgcttca cgctcggcaa
      721 cgcgggggag cgctaccaag accgctactg gcacgagcgc gagttcgaga cggtggaggc
      781 gctccggccg ctggccgccg aggcgggcat gccgctcgcg cggctcgcgg tggcgtggat
      841 gctcgccgag cccgcgatca cctcgccgat cgtgggggcg agccgccccg accagctcga
      901 cgacgtgctg cccgcgaccg agaagccgct ggacgccgcg ctcaaggcgc gcctcgacga
      961 gatgactcgc gagtaccgct ggggcgatga tccacgctga tcggaggagc aaggccatgg
     1021 ctgcgtccgt gaaccgccag atcgtgctcc gcagccgtcc cgtcggcatg cccaggcccg
     1081 ccgacttcga tctcgtcgag tccccgatcc ccgcgccgaa ggacggcgag atcctgagcc
     1141 ggaccatcta cctctcgctc gatccgtaca tgcgcgggcg catcagcggc gcgcgctcgt
     1201 acgcgcagtc ggtggagccc gggcaggtga tcgtgggcgg caccgttggc ctggtcctcg
     1261 agtcgaagca cccgggcctc gcgaaaggcg acatcgtgct gggctacgac ggctggcagt
     1321 cccacgcggt ggccaagggc gcggcggtgc gcaagctcga tccgaggcag gcgcccgtgt
     1381 ccaccgcgct gggcgtcctc ggcatgccgg gcatgaccgc ctacgtgggc ctgctcgaca
     1441 tcggccagcc caggccgggc gagacggtgg tggtctccgc cgcctcgggg gcggtcggct
     1501 cggcggtggg gcagatcgcc aggatcaagg gcgcccgcgc ggtgggcatc gcgggatccc
     1561 aggacaagtg tgactacgtc gtgcgcgagc tcggcttcga cgcctgcgtg agctacaaga
     1621 agggcgatct gctcgcctcg ctcaaggagg cctgcccgaa cggcatcgac gtgtacttcg
     1681 agaacgtggg cggcgacgtc ctgcgggcgg tgatgcatct gctgaacatg aacgcgcgca
     1741 ttcctctctg cgggctcatc tccgagtaca acgcgaccga atcggtcccc ggcccgaacc
     1801 tgcgccccct gctgttcaat cgcgcgctcg tgaagggctt catcgtgtcg gaccacatgg
     1861 cccgcctgcc cgagttcctg aaggactgcg gcggctggct gcgcgagggc cggctcaagc
     1921 accgcgagga catcgtcgcg ggcctcgaga aggcgccgga agcgttcatc ggcctgctgc
     1981 agggcaagaa cttcggcaag ctgctggtgc gcgtggcgga ggaccccacc cggtgagccg
     2041 gcgcgggcgc ctggcggtcg cggccgccct cctggccctc ggcctggggc cggcggtctg
     2101 ctccgcgcag gtcttcatcg cgtccaagcc caatcccgag ttctggatcg cgccggtcct
     2161 catcaccgcg aacgtcaagc ggaaggacgt ggacgaccgt cccggcccgc tcatgatcca
     2221 ggtctcgttc agcgtggccc cgccgccggc gcgcgacccg gccgagttcg cccaggacat
     2281 ctttctcctg tggccgggcg agctggtcgg caccgacggc gtcgacggcg ccgatcctct
     2341 gctgctgcgg caggtggagg ccgccggctt caaggtgctc gtgcacggcc gcgtcccgtt
     2401 ctccgcccgg gaccggcgcc agatgggcac cggagcgggc gccgccggcc ggcgcgatct
     2461 cggcagcgcc ttcttcgtca ccttcgcccg gccggagggg ctcgcccgcg gcgcgaagcc
     2521 cgcgacctac gtccgcatcc cgtggaagcc cgaggtggcg agcctcgact ggatcccccg
     2581 cctggagctc gcggccaagg gcgccatcac gccccgccgg gtgagctggc tcgaggaagc
     2641 gttctggggc cggcgcaaca tcatcacgct gagcttcggc gacgtcggct actcctccct
     2701 gtatccgttc tactacggca accgcgatcg cgtcatcccg ctcgccgccg acttctcccg
     2761 cctcatgatc aacttcgcgg aggcgaacca tctcaagatc gacgaggtga tcccgtccac
     2821 cgcgctccgc cggatgagcg agacgcggga gaacacggac acgttctcga ttcccctcat
     2881 cgcggcggac ggcatcgtgc cgcagctgct gaaggtgcag ttcgtctact tccaggggcg
     2941 gctaccgtgg cgccccatcc tcctctccgc gctcctcctg ggcctgggca atctcaccgg
     3001 cccgatcgtc ggcaacctgc ttcgcaagct catccggacc ctgcgagacc gggtgcacgt
     3061 cgggcgcggc gaggtcaccg ggcgggcccg cggcgaggtg ccctcgcagg agacgctggc
     3121 ccgcatccgg cccggggaga cgacctacca ggacgtgatc cgggtggtgg gcagcgtgcc
     3181 cgaggaggag cagcgcctgc cctcgggcga gatccgagcg gtgatctacc gcggccagcg
     3241 cctggtgccg caccggggtc ggcggttcgg ctggttcgcc accgtgcgcc actgggagct
     3301 ggagaaccac gaggtccaga tcgacttcga gcaggatcgg gtgcgcgata tccaggcgcg
     3361 catccgccgc acgcgccaga cgccgtccac gtccgcggcc gtctgagccg cgcccggcgg
     3421 ggtccgtcag tgggcgcgga tccaggcgcc gatcgccgag accacctgcg cctcgaggcc
     3481 gaggtagccg tggggggctt tcgcctcgca cggcgccgag atgggcgggc tccccccctc
     3541 gaaggccatc agctccttga ctggcgcgcg cttgagggcc ttcaggatgt cctccgcgcc
     3601 ggaatagggt gaggaccggc aggtgtcgcc cttgtggtgg accacgagcg tgggcacgcg
     3661 gatgtcgccg agtccggcgt gccgcaccgc ctcgtagctc atcttcgagg tctcggtgac
     3721 cgaggaggtg agcacgatgc cgtccggccc gccctcggcg agccgggccg ccgcgttggc
     3781 cgcggagagt gtgcccatgc tggtgcccac gagccacacc ggcgcccggg cgatctcgcg
     3841 cagcgccgcg atggcctgct tgacgtccgt cgcgtgctcg ggcgtcgtgc ggaagttcca
     3901 gaggccgctc ttccggtcgg acggctggtc gaggacggcc accaggaagc cctcgcgagc
     3961 ccagcgcatc cgggtccgca cgaggaaatt gccctggagc tggccgatcc cgccggcgct
     4021 cagcgcgagg ttgccgtcgc cgcccgcgaa gatgatcacg ctggccaccg gcgggccggc
     4081 cgggcgcagg aggaggaagg actcggtgac gcccggccgc gccgggatcg tgcgaacctc
     4141 atcgtcggcg gcccgcgcgg ccgatacggg gagcgtggcg gccaggagga ggacgacggc
     4201 gacgaggcgt gggcgcatgg gctgaccgga tgataggcgg cgcgccggac gctggcaagg
     4261 cggggcgccg cgttgtacag tccggggcca gggaggccgc gatcaatggc gacgatctac
     4321 gacgaggtgt accggcgcgc gcacgacgac ccggagggct tctgggccgc gcgcgccgag
     4381 gacatccact gggagaagcg ctgggaccgg gtgctcgacg actcgcgccc gccgtactac
     4441 cgctggttcg cgggcggggt gctgaacacc tgctacaacg cgctcgatct ccacgtggac
     4501 cgcggccgcg gcaagcagac cgccctgatc tacgactccc cggtgacccg gaccatccgc
     4561 gggttcacct acttcgagct gcgcgacgag gtcgcgcgct tcgcgggcgc gctgcgccgc
     4621 cagggggtgg cgaagggcga ccgagtgatc atctacatgc cggcggtgcc ggaggcggtg
     4681 atcgcgatgc tcgcgtgcgc gcgcatcggg gcgatccact cggtggtgtt cggcggcttc
     4741 gcgccaaagg agctggccac ccgcatcgac gacgcgaagc cgaacgtcat gctctcggcc
     4801 tcctgcggca tcgagggcaa ccgggtgatc ccctacaagc cgctcctgga ccaggccatc
     4861 gcgatggcct cgcacaagcc ctcgcgctgc ctgatcctgc agcggccgat ggagaccgcg
     4921 tcgctcgtgc ccggccgcga cgtggactgg ggggaggcgc tggccgcggc tgggcatgtc
     4981 gagtgcgtgc cggtggcggc cacggatccg ctctacatcc tgtacacctc gggcaccacc
     5041 ggcattccca agggcgtggt ccgcgacaac ggcggccacg cggtcgcgct caagtggagc
     5101 atggacgcgg tctacggcat gggggcgggg gagacgttct gggcggcctc cgacatcggc
     5161 tgggtggtgg gccactccta catcgtctac gcgcccctcc tcaagggctg cacgacgatc
     5221 ctgtacgagg gcaagccggt gggcacgccc gaccccgggg cgttctggcg cctctgcgcc
     5281 cagcacgggg tgagcgcgat gttcaccgcg ccgaccgcct tccgcgcgat caagaaggaa
     5341 gacccgcagg gcgcccacat cggcaagtac gacctctcgc gcttccgcac gctcttcctg
     5401 gccggggaac gctgcgatcc cgacaccctc ctctgggcgc gcgagaagct tcaggtcccg
     5461 gtggtggacc actggtggca gaccgagacc ggctggccga tggcggccaa ctgcgtggga
     5521 ctcgggatgc tgccggtcaa gccgggctcg ccgaccaggg tcgtgccggg ttacgacatc
     5581 cgcgtgctgg gcgaggacaa ccgcgagatg ccggcgggcg agatcgggtc catcgcggtg
     5641 aagctgccga tgccgcccgg cagcctgccc acgctgtggc acaacgacga gggcttcgag
     5701 cgctcgtacc tcacgcggca tccgggctac tacctgaccg gggacgccgg ctacaaggac
     5761 gaggacgagt acatctacat catgagccgc gtggacgaca tcatcaacgt ggccgggcac
     5821 cggctctcca ccggcgccat ggaggaggtg ctctccgcgc atccggacgt ggccgagtgc
     5881 gcggtgatgg gcgtggccga cgagatcaag ggcgaggtgc cggtggggct agtggtcatc
     5941 aaggcgggcg tggcgcgcgc tgacgccgac ctctgccgcg agctggtcga gctggtgcgc
     6001 gagaagatcg ggccggtcgc cgcgttcaag acggtggcgg tggtcaagcg gctgcccaag
     6061 acccgctccg gcaagatcct gcgcgggacc atgaagaaga tcgccgatgg ggtcgagcac
     6121 gccgtgcccg ccaccatcga cgatcccgcg atcctcggcg agatcagcga ctcgctgaag
     6181 gccctcggct acccgaagta ggaggcggcc tccggtacgc ggcgcggcgg aacttaccgg
     6241 gcgagatcga ccagcgtgtg ctcctcgccg aaccagatgg gcgagcgcgc gagatcgatg
     6301 aagcgcttct catcctcgag cagccggcgc cccgccgcgc ggcccgcggg cgtcgcgccc
     6361 gcgcgggcga actcttccac ggtctcccac cagagctcgg ccacgccgtc gaagtcctcc
     6421 ggcgcgccgc ggtggcggcg catcgcctcg ctgaaggcgg acgtcaccgt gtgcacctgg
     6481 gtgtagcgct tgatccccag ggcgggggcc agctcccgca ccagcggccc gtgggtgtcg
     6541 agccagtagc gctggaactc ctcgcgggac aggtggggca ggcggcggag gcagaacacg
     6601 agctt
//
LOCUS       DASUFA010000134         6208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_718912, whole genome shotgun sequence.
ACCESSION   DASUFA010000134 DASUFA010000000
VERSION     DASUFA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718912"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..792
                     /locus_tag="VFX87_04920"
     CDS             58..792
                     /locus_tag="VFX87_04920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX4994285.1"
                     /translation="MVIAAAREIRDDDVVFVGMRLPLIAFAYAKRTHAPGATGLFENG
                     IVRDRPAPETLMTMSDTPNIPGALWCTGTLEIMAFLQQGLVDLGFIGGAEIDRFGNLN
                     TSYIGDWRRPKVRLPGSGGGGDIASLARRFVVIMPQEKHRFRERVDYITSPGFGEGPG
                     WRARVGLTGGGPAAVVTTVGVFRFDPASCEMYLASYHPGQSADSVRAATGFTLRTAPD
                     VGPTPTPTAAELAIVRECDPAGFWTR"
     gene            795..2132
                     /locus_tag="VFX87_04925"
     CDS             795..2132
                     /locus_tag="VFX87_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994286.1"
                     /translation="MTTADPAAPAGRRWLMWGIPALIFLIAFLHRAAPGVLAKELMQA
                     FGITGTVVGLLSAMYFYSYAGFMVPGGVLIDSVGPRWVIAGGGAVMSLGSLAMGLAPG
                     PAVLFAGRLLVGLGATVTFTGALKIAANWFPRSQFGMMSALTATVGVLGALVGSAPLA
                     ALAGIVSWRGALVIIGALTLAAAILCVAAVRDRPAGARDASPHAPSLRVVLAGTLQVL
                     RNPYTWPPFLCFFFLYAAMGNFFLWSVPFLRDVYGLTTTKAALYASLPSVALLVSAPL
                     TGYLSDRVLHRRKLPYIILVTGQFVVWLAFLLTLGTLPLSGVCVLFVLLGTVGGAFVL
                     TWPMGAEVNPPELAGSAVAVTNLGGFVGAALTQGPVGAVLDAGWTGALVEGARVYPVA
                     SYRGAFALCVLFVLLGAAISLLLKETRGENVHHRLRPAPLSAETEPRRARGES"
     gene            complement(2026..2637)
                     /locus_tag="VFX87_04930"
     CDS             complement(2026..2637)
                     /locus_tag="VFX87_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4994287.1"
                     /translation="MTESRIAPLEPPYPEPVRAALDRIMPKDVPPLALFRILAVNERV
                     FLRVMAGGLLDRGSISLREREIVIDRTCARCGSEYEWGVHVAFFAERVGLTASQVAAT
                     CAPEAEAGAFPERERLLIRLADALHDEARVDDALWAALRAQWTEPQLIELIALAGFYH
                     LISFVTNALRIPPEPYGARFPRRAGPDAAGDGRSRPASPSAAS"
     gene            2728..3090
                     /locus_tag="VFX87_04935"
     CDS             2728..3090
                     /locus_tag="VFX87_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4994288.1"
                     /translation="MARTPRPGRPVRGSRTGRPIMALLDLLSRRWTLRVIWELRGGRL
                     TFRALREACGGVSPTVLNERLRELRESGLLESAGAAGYGLTPLGRELIEQFLPLAAWS
                     TRWAAAITRARGTPRSSR"
     gene            complement(3053..3781)
                     /locus_tag="VFX87_04940"
     CDS             complement(3053..3781)
                     /locus_tag="VFX87_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4994289.1"
                     /translation="MATNILLARDGPIATVTLNRPDRRNSLSDEMLTDLGAAFAELRD
                     DASTRVVIVTGASPVFSAGADAGHAKAATPEERRRLFLSRKSQFRRLFERSNALLENL
                     EQATIAAVNGHAVGGGWGLALACDFRIAAAEAQFWIPEVDLGVPLGIGTTTRFVRMVG
                     PARAKEIIMGCARYSAADAQAWGLVNRVVPGAELAQAARDYAEILARKPFRPLAEVKA
                     RINAIARIGIPEVNAMTEGFLERE"
     gene            complement(3845..5362)
                     /locus_tag="VFX87_04945"
     CDS             complement(3845..5362)
                     /locus_tag="VFX87_04945"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEX4994290.1"
                     /translation="MSILIAALGGQGGGVLTEWLVGAAGHAGYPAQATSIPGVAQRTG
                     ATTYYVEVFPERIEAGGPEPIFSLYPTPGDVDVIIASELLEAGRTIEMDYASPGRTTL
                     VASTHRLFSIAEKSVPGDGIYPSEGLEAAARKLSRRFVGFDALTAARRHQTEVNALLL
                     GALSATGVLPMTAADFEAAIREGGVAVERNIRGLKAGQELVATGPPGTEAPPAERPWP
                     VVKAERAAALGPRGAGFLALATRTEAEFPDPLHATLGEALARLIDYQDVRYAELFLER
                     VRRVCALDAGGRLTRVFARRLAVWMTYEDAIRVADLKTRRGRFERIRGENAAPAGSVV
                     VVTDYLKPDLDELYGILPAAIGGPIARWAERRWPEGRPTIGQHVKTTSVLGFLRVRLL
                     ARLRWLRPSSLRRQREFALMGRWEDAVLAAAALDLALAAEVAELANIVKGYGEVRRRL
                     SGALERFLDDTVRPGIEADRRAGAGYARSAELVRAGRHRLLADENASSLIPHGRG"
     gene            complement(5380..>6208)
                     /locus_tag="VFX87_04950"
     CDS             complement(5380..>6208)
                     /locus_tag="VFX87_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4994291.1"
                     /translation="GETHVAADIGCHAFATLPPFNQGNTILGYGMSVASASAVAPNFG
                     KRVISILGDGGFWHSGLTAGVANAVFNKQDSIVVVLENFYTSATGQQANPSSGKNPRG
                     DAVKMSIADTIRSLGVSWIKTVNPYRVGDTLKVVKEAMTTAAGGLKVIIARAECQLER
                     QRRVKPETAARLKAGARVEVPRFGVDPDVCTGDKSCMRFNGCPSLTLKDNPDPLRDNP
                     VAHVDASCVGCGLCGEVAHAAVLCPSFYEARVVHNPGAGSRALDGLRRRVIGWLQPA"
BASE COUNT          795 a         2253 c         2259 g          901 t
ORIGIN      
        1 ccgcgcaccc gcgctatgaa agagaagctc ccgggagatt tcaccgagcg cgaattgatg
       61 gtcatcgcgg ccgcgcggga gatccgggac gacgacgtgg tcttcgtggg catgcgcctg
      121 cccctgatcg cgttcgccta cgccaagcgc acgcacgcgc ccggggccac cggcctcttc
      181 gagaacggca tcgtgcgcga tcggccggcg cccgagacgc tgatgaccat gagcgacacc
      241 ccgaacatcc cgggcgccct ctggtgcacg ggcacgctcg agatcatggc gttcctccag
      301 caggggctcg tggatctcgg gttcatcggt ggggccgaga tcgaccggtt cggcaacctg
      361 aacacctcct atatcgggga ctggcgccgg ccgaaggtgc ggctgcccgg cagcggcggc
      421 gggggcgaca tcgcgagcct ggcccggcgc ttcgtggtga tcatgccgca ggagaagcac
      481 cggttccgcg agcgcgtgga ctacatcacc tcgcccggct tcggcgaggg cccgggctgg
      541 cgcgcgcggg tcgggctcac ggggggcggg cccgccgcgg tggtcaccac ggtgggggtc
      601 ttccgcttcg accccgcgag ctgcgagatg tacctggcct cctaccatcc ggggcagagc
      661 gcggacagcg tccgggccgc caccgggttc acgctccgga ccgcgcccga cgtggggccg
      721 acgccgacgc cgaccgccgc ggagctcgcc atcgtccgcg agtgcgaccc cgccggcttc
      781 tggacccgct gagcgtgacc acggccgatc cggccgcccc ggcgggccgg cggtggctca
      841 tgtggggcat cccggccctc atcttcctca tcgccttcct gcaccgcgcg gcgcccggcg
      901 tgctggccaa ggagctcatg caggcgttcg gcatcaccgg cacggtggtg ggcctgctct
      961 ccgcgatgta cttctactcg tacgcgggct tcatggtgcc gggcggagtg ctgatcgaca
     1021 gcgtcggccc gcgctgggtg atcgcgggcg gcggcgcggt catgagcctg ggttcgctgg
     1081 ccatggggct cgcccctggt cccgccgtcc tcttcgccgg ccgcctgctg gtgggcctgg
     1141 gcgcgaccgt gaccttcacg ggcgcgctga agatcgcggc gaactggttc ccgcgctcgc
     1201 agttcgggat gatgtccgcg ctgaccgcga cggtgggcgt gctcggcgca ctcgtgggca
     1261 gcgcgcccct cgccgcgctg gccgggatcg tgtcctggcg cggcgccctc gtcatcatcg
     1321 gcgcgctcac gctggcggcc gcgatcctct gcgtggcggc cgtccgcgac cggcccgcgg
     1381 gcgcgcgcga tgcttcaccc cacgcgccgt cgctccgggt cgtgctggcc ggcacgctcc
     1441 aggtgctgcg caacccgtac acgtggccgc cgttcctctg cttcttcttc ctctacgccg
     1501 cgatgggcaa cttcttcctg tggagcgtgc cgttcctccg cgacgtctac ggcctcacca
     1561 ccaccaaggc cgccctgtac gcctcgctgc cctcggtcgc gctgctcgtc tccgctccgc
     1621 tcaccggcta cctgtccgac cgggtcctcc accgccggaa gctcccctat atcatcctgg
     1681 tcaccgggca gttcgtggtc tggctcgcgt tcctgttgac gctcggcacc ctgccgctct
     1741 cgggcgtctg cgtgctcttc gtgctccttg gaaccgtggg cggcgccttc gtgctcacct
     1801 ggccgatggg ggccgaggtg aacccgcccg agctcgcggg cagcgcggtg gcggtcacca
     1861 acctgggcgg cttcgtgggc gccgcgctca cccaggggcc ggtgggcgcc gtgctcgacg
     1921 cgggatggac gggcgccctc gtcgaggggg cccgcgtgta cccggtggcg tcctaccggg
     1981 gcgccttcgc gctctgcgtg ctcttcgtgc tcctcggcgc cgcgatcagc ttgctgctga
     2041 aggagacgcg gggcgagaac gtccatcacc ggctgcgtcc ggccccgctc tccgcggaaa
     2101 ccgagccccg tagggctcgg ggggaatcct gagcgcgttg gtgacgaacg agatcaggtg
     2161 gtagaagccg gcgagcgcga tcagctcgat gagctgcggc tcggtccatt gcgcgcgcag
     2221 cgcggcccac agcgcgtcgt ccacccgggc ctcgtcgtga agggcatccg ccagccggat
     2281 cagcagccgt tcccgctccg ggaacgcgcc ggcctccgcc tcgggcgcgc acgtggcggc
     2341 cacctgcgac gcggtgaggc cgacccgctc ggcgaagaac gccacgtgca cgccccattc
     2401 gtactcggag ccgcagcgcg cgcacgtccg gtcgatcacg atctcccgct cgcgcaggct
     2461 gatcgagccc cggtccagca ggccgcccgc catcacgcgc aggaagaccc gctcgttgac
     2521 cgcgaggatg cggaagagcg cgagcggcgg cacgtccttc ggcatgatgc ggtcgagcgc
     2581 ggcccggacc ggctccgggt acgggggctc gagaggggca atgcggctct cggtcatggc
     2641 gtcggtcctc tctgacgtga ggcgtgctat catttcagta gcatccatac taccgctact
     2701 tattcagtag cacaagggag gcgcccgatg gccaggaccc ccaggccggg ccggccggtg
     2761 cggggctcgc ggacggggcg gccgatcatg gccctcctgg atctgctgag ccggcgctgg
     2821 acgctgcggg tgatctggga gctgcgcggg ggccgcctca ccttccgggc gctccgggag
     2881 gcctgcggcg gcgtgagccc caccgtgctc aacgagcggc tgcgcgagct gcgcgagagc
     2941 gggttgctgg agtcggcggg cgcggcgggc tacggtctca cgccgctggg ccgcgagctg
     3001 atcgagcagt tcctgccgct ggccgcctgg tcgacccggt gggccgccgc gatcactcgc
     3061 gctcgaggaa cccctcggtc atcgcgttga cctcggggat gccgatgcgc gcgatcgcgt
     3121 tgatgcgggc cttgacctcg gcgagcggcc ggaacggctt ccgggccagg atctccgcgt
     3181 agtcgcgcgc ggcctgcgcc agctcggccc cgggcaccac gcgattgacg aggccccagg
     3241 cctgggcgtc ggccgccgag tagcgcgcgc agcccatgat gatctccttg gcgcgcgccg
     3301 ggcccaccat ccgcacgaag cgggtcgtgg tgccgatccc gagcggcacc cccaggtcca
     3361 cctcggggat ccagaactgc gcctcggcgg cggcgatgcg gaaatcgcac gcgagcgcca
     3421 ggccccagcc gccgcccacc gcgtggccgt tgaccgcggc gatggtggcc tgctcgaggt
     3481 tctcgagcag ggcgttcgac cgctcgaaga ggcggcggaa ctgcgacttg cgtgagagga
     3541 acagccggcg gcgttcctcc ggggtcgccg ccttggcgtg tcccgcgtcg gcgccggccg
     3601 agaagaccgg cgacgcgccg gtgacgatga cgacgcgtgt ggacgcgtcg tcgcgcagct
     3661 cggcgaaggc cgcgcccagg tcggtgagca tctcgtcgct cagcgagttg cggcgatcgg
     3721 ggcggttgag cgtgacggtg gcgatgggac cgtcgcgggc gaggaggatg ttggtggcca
     3781 tgcgggcgga atgtaccatg acccttcgga tgttgcgcag aatcgacggc tgggggagat
     3841 cccctcaccc tctcccgtga gggatgagag aagatgcgtt ctcgtcggcg aggaggcggt
     3901 ggcggccggc gcggacgagc tcggccgagc gcgcgtagcc cgcgccggcg cgacggtcgg
     3961 cctcgatccc ggggcgcacc gtgtcgtcga ggaagcgctc gagcgcgccg gagaggcgcc
     4021 gtcgcacttc gccatagccc ttcacgatat tggccagctc cgcgacctcc gcggccaggg
     4081 ccagatcgag cgcggcggcc gccagcaccg cgtcctccca gcggcccatc agcgcgaact
     4141 cgcgctggcg ccgcagcgac gacgggcgca gccagcggag gcgagcgagc agccggacgc
     4201 gcaggaagcc cagcaccgag gtggtcttca cgtgctggcc gatcgtcggc cggccctcgg
     4261 gccaccgccg ctccgcccag cgcgcgatgg gccctccgat cgcggcgggc aggatgccgt
     4321 agagctcgtc cagatcgggc ttgagatagt ccgtgaccac gaccaccgag cccgcgggcg
     4381 ccgcgttctc gccgcggatg cgctcgaagc gaccgcggcg cgtcttgagg tcggccacgc
     4441 ggatggcgtc ctcgtaggtc atccacaccg caagccgccg cgcgaagacg cgcgtcagcc
     4501 gcccgcctgc gtcgagcgcg cacacgcgcc ggacgcgctc gaggaacagc tccgcgtagc
     4561 ggacgtcctg gtagtcgatc aggcgggcga gtgcctcgcc cagcgtcgcg tggagcgggt
     4621 ccgggaactc ggcctcggtc cgcgtggcca gcgccaggaa gccggcgccc cgcggcccga
     4681 gcgcggccgc ccgctccgcc ttgacgaccg gccacggccg ctcggccggc ggcgcctccg
     4741 tccccggcgg cccggtcgcc accagctctt ggcccgcctt caggccgcgg atgtttctct
     4801 ccaccgcgac gcccccctcg cggatcgcgg cctcgaagtc ggccgcggtc atcggcagca
     4861 cgccggtggc cgacagcgcg cccagcagca gcgcgttgac ctcggtctgg tgccggcgcg
     4921 ccgccgtcag ggcgtcgaag cccacgaagc gccggctgag cttgcgggcg gcggcctcca
     4981 gaccttcgct cgggtagatc ccgtcgcccg gcaccgactt ctccgcgatc gagaagagcc
     5041 ggtgcgtgct cgcgaccagc gtggtgcggc cgggcgacgc gtagtccatc tcgatggtgc
     5101 gcccggcctc gagcagctcc gaggcgatga tcacgtccac gtcgcccggc gtcgggtaga
     5161 gcgagaagat cggctcgggg ccgccggcct cgatccgctc ggggaatacc tcgacgtagt
     5221 aggtggtggc gccggtgcgc tgggccacac ccgggatcga ggtggcctgg gccgggtagc
     5281 ccgcatggcc ggccgcaccc acgagccact cggtgagcac gccgccgccc tggccaccga
     5341 gcgcggcgat caggatcgac acggggcggc gcgaagccat cacgcgggct ggagccagcc
     5401 gatgacgcgc cgccgcagcc cgtcgagcgc gcgggagccg gcgcccggat tgtgcacgac
     5461 gcgcgcctcg tagaacgacg ggcacagcac cgcggcgtgg gccacctcgc cgcagaggcc
     5521 gcagcccacg cagctcgcgt ccacgtgggc caccggattg tcgcgcagcg ggtcggggtt
     5581 gtccttcaag gtgagagacg ggcagccgtt gaagcgcatg cacgacttat cgccggtgca
     5641 gacgtcgggg tccacgccga atcgcgggac ctcgacccgc gcgcccgcct tgagacgggc
     5701 cgccgtctcc ggcttcacgc gccgctggcg ctcgagctgg cactccgcgc gcgcgatgat
     5761 gaccttgagc ccgcccgccg cggtggtcat cgcctccttg accaccttca gggtgtcgcc
     5821 gacgcggtag ggattcacgg tcttgatcca ggacaccccg aggctccgga tcgtgtcggc
     5881 gatggacatc ttcaccgcat cgccgcgcgg gttcttgccc gacgaggggt tggcctgctg
     5941 gccggtggcc gaggtgtaga agttctccag caccaccacg atcgagtcct gcttgttgaa
     6001 gaccgcattg gccacgcccg cggtgaggcc cgagtgccag aagccgccgt cgccgaggat
     6061 cgagatcacg cgcttgccga aattcggcgc gaccgcgctc gcggaggcca cgctcatccc
     6121 gtagcccagg atcgtattgc cctggttgaa cggcggcagc gtggcgaagg cgtggcagcc
     6181 gatgtcggcg gccacgtggg tctcgccg
//
LOCUS       DASUFA010000135         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_144169, whole genome shotgun sequence.
ACCESSION   DASUFA010000135 DASUFA010000000
VERSION     DASUFA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144169"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..2118)
                     /locus_tag="VFX87_04955"
     CDS             complement(7..2118)
                     /locus_tag="VFX87_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX4994292.1"
                     /translation="MRRLTAVACLMSVVLVGAAPAGLSPDLRERFATGLAAHKAGDWA
                     TAGREFADPVWAGTPLEDYALLFQAESQLRLGDVVAARALATQAADRTPASGLTPSAL
                     MRAASVRREAGDPAGAVTTLQRVVTRLADGPDAARARYALGEALLASGDQREAARVFQ
                     ALWLQAPAALGDATEGQLKALADAGVAPPPPSAVERAARADRLLASGLIERARLEAEA
                     LVAEKPAADALERALRVLMNASRRLGRDDAALAAANDGLAAAAPERRASWLLEQARLR
                     QRRDREGALATLDRLVRDYPKSNAAPAALLLKAELLEAVARLAEGEKTYVKLAADYPD
                     EDDAANALWRLGWIAWFRGNAVEATARWSRVQVARGGQSLREAATYWVGRAWERRGDR
                     DQAGRQFAQLVKDSPRTYYGLLAAKRAPGAGRGAAAGEPSGSGAGRGAAAGEPSGSGK
                     SGTAPFTFPTDPREGLQGDPRFDRAVALREVGLGEFADEEMDELTRRSVGEPRRLYAL
                     SAAYVADERYHMALRILRRSFQGAARSGGASPREFWEMFYPLGWRDALTAAAGRASLD
                     PFLVAAVVREESSFYPRARSRVGARGLMQLMPDTGREVARVRQIPFPDAEVLDQPGTN
                     LEIGSIFFGGLFREFGDARLAAAAYNAGPTRVREWWANRKTDDLEIWVEQIPYNETRA
                     FVKRVMLSWQEYQRVYSEKPS"
     gene            complement(2164..2754)
                     /locus_tag="VFX87_04960"
     CDS             complement(2164..2754)
                     /locus_tag="VFX87_04960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4994293.1"
                     /translation="MLSHYKASLTRLGDPVARALLRAHVRPNHLTMVGLGVSILAACA
                     LAQGQLRVGAGLLALAGLFDFFDGSLARLANSVSAFGAFLDSVVDRYSDLVVLLGVVL
                     HYHRAADTTGVCLTMGALVGTIMTSYTKARAQSIGVPCEIGLIERPERLIVLIAGATF
                     NLLTPAIIALAVLTNLTALQRILYMRRAAPRERILH"
     gene            complement(2755..>3960)
                     /locus_tag="VFX87_04965"
     CDS             complement(2755..>3960)
                     /locus_tag="VFX87_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX4994294.1"
                     /translation="PDSEGNAQVVSELERPGLLMLGMLLHDIGKGKGHGHVAKGIPLI
                     EALSARIGLPAEDADKVVFLVAHHLTMSHVAQRRDIDDPKTIETLAEVCRTPERLRML
                     YLLTCADMRAVGPGVMTGWQAQILWDLYARTLARLTGGQRERPTRESVAQRVTAAVRG
                     EVARTAVAVHLALLSDRYLATTSPQRIAAHLRLLDQLTDGGVLATELFHHPDLGSSEL
                     VVATRDVPGLFSLIAGTLASQGINILSAQIHTRADGIVLDTFQVNDPFGEAVTEEARW
                     RRTLEALRRVLRGEVSVEDLLARRRAVYPSGEGVAGPPKISVDNQISDSRTVVEVKCP
                     DRVGLLYVITRTLSGHGLDIASARIATEIDQAYDTFYVTDRQGRRLEDEAAMARVRES
                     LEDALLKPL"
BASE COUNT          554 a         1462 c         1435 g          509 t
ORIGIN      
        1 ggcccatcag gacggcttct cgctgtacac ccgctggtac tcctgccagg agagcatcac
       61 gcgcttcacg aacgcgcgcg tctcgttgta ggggatctgc tcgacccaga tctcgaggtc
      121 gtccgtcttc cggttcgccc accattcgcg cacccgcgtg ggaccggcgt tgtacgcggc
      181 ggccgcgagc cgcgcgtcgc cgaactcgcg gaagagcccg ccgaagaaga tcgagccgat
      241 ctcgagattg gtccccggtt ggtcgagcac ctccgcgtcc gggaacggaa tctggcggac
      301 ccgggccacc tcgcgtccgg tgtcgggcat gagctgcatg agcccgcggg cgcccacccg
      361 cgagcgggcc cgcgggtaga acgaggactc ctcgcgcacc accgccgcga ccaggaacgg
      421 atcgagcgag gcccggcccg cggccgcggt gagagcgtcg cgccagccca gcgggtagaa
      481 catctcccag aactcacggg gcgacgcccc gccgctgcgg gcggcgccct ggaagctccg
      541 ccgcaggatg cgcagcgcca tgtggtagcg ctcgtcggcg acgtaggcgg ccgagagcgc
      601 gtacagccgg cggggctcgc ccaccgagcg gcgggtcagc tcgtccatct cctcgtcggc
      661 gaactcgccc agccccacct cgcgcagcgc gaccgcgcgg tcgaagcgcg ggtccccctg
      721 cagcccctcc cgcggatcgg tggggaatgt gaagggcgcc gtacctgact taccggatcc
      781 gctcggctcg cctgcggctg cgcctcgccc agcaccggat ccgctcggct cgcctgcggc
      841 tgcgcctcgc ccagcaccgg gcgcacgctt ggccgcgagc aggccatagt aggtgcgcgg
      901 cgagtccttc acgagctgcg cgaactgacg gcccgcctga tcccgatcgc cccgtcgctc
      961 ccacgcccgg ccgacccagt aggtggcggc ctcgcgcagc gactgcccgc cgcgcgcgac
     1021 ctggacgcgg gaccaccgag cggtcgcctc cacggcattg ccgcggaacc aggcgatcca
     1081 gccgaggcgc cagagcgcgt tggccgcatc gtcctcgtcc gggtaatcgg cggccagctt
     1141 cacgtaggtc ttctcgccct cggcgagccg ggccacggcc tcgagcagct cggccttcag
     1201 caggagtgcg gccggcgcgg cgttgctctt gggataatca cgcacgagcc ggtccagcgt
     1261 ggcgagcgcg ccctcgcggt cgcgacgctg ccggaggcgg gcctgctcga gcagccagct
     1321 cgcgcggcgc tcgggcgccg ccgcggccag gccgtcattg gcggcagcca gcgcggcgtc
     1381 gtcccggccg aggcggcgcg aggcgttcat cagcacccgc agggcgcgct ccagggcgtc
     1441 cgcggccggc ttctcggcga ccagggcctc ggcttccagc cgggcccgct cgatcagccc
     1501 cgaggcgagc agccgatcgg cgcgcgcagc ccgctccacc gcgctcggcg gaggcggcgc
     1561 cacgccggca tccgccagcg ccttgagctg gccctcggtc gcgtcgccga gggcggcagg
     1621 cgcctgcagc cacagcgcct ggaacacgcg cgccgcttcc ctctgatcac ccgaggccag
     1681 gagggcttca cccagcgcgt agcgcgcccg cgccgcgtcc gggccgtcgg ccaggcgtgt
     1741 aaccacgcgc tgcagggtcg tcaccgcccc ggccggatct cccgcctcgc gccgcacgga
     1801 ggccgcccgc atcagggcgg agggcgtgag cccacttgcc ggcgtccggt cggcggcctg
     1861 ggtggccagg gcccgcgccg cgaccacgtc ccccaggcgg agctggctct cggcctggaa
     1921 gagcagcgcg tagtcctcga ggggggttcc ggcccacacg ggatcggcga actcccggcc
     1981 cgcagtggcc cagtcgcccg ccttgtgggc ggccagcccg gtcgcgaacc gctcccgtag
     2041 atccggggaa aggcccgccg gcgccgcccc caccagcacc acggacatca gacacgccac
     2101 cgcggtcagc cgcctcatag ctcccagtgt accagccgcc accacgccga ccgagctagc
     2161 cggtcagtgg aggatccgct cgcggggggc ggcgcgccgc atgtagagga tgcgctgcag
     2221 cgcggtcagg ttggtgagaa cggcgagcgc gatgatggcc ggggtgagca ggttgaacgt
     2281 ggcgcccgcg atgagcacga tcagccgctc ggggcgctcg atcagcccga tctcgcaggg
     2341 taccccgatg gactgggcgc gggccttggt gtagctggtc atgatggtgc ccaccagggc
     2401 gcccatggtg aggcagacgc cggtggtgtc ggccgcacga tggtagtgga gcaccacccc
     2461 cagcagcacg accaggtcgg agtagcggtc caccaccgag tcgaggaagg ccccgaaggc
     2521 gctcacgcta ttggcgagcc gcgcaagcga gccgtcgaag aagtcgaaga gcccggccag
     2581 cgccagcagc cccgcgccca cccgcagctg tccctgggcg agcgcgcacg cggccaggat
     2641 gctgaccccg agcccgacca tcgtgaggtg attcggccgg acgtgcgccc ggagcagggc
     2701 gcgcgccacc ggatcgccga gccgggtcag cgatgccttg tagtgactca gcatctagag
     2761 cggcttgagc aaggcgtctt ccaggctctc gcgcacgcgg gccatcgccg cctcgtcctc
     2821 gaggcgacgg ccctgccggt cggtcacgta gaaggtgtcg taggcctgat cgatctcggt
     2881 ggcgatccgc gcgctcgcga tgtcgagccc gtgccccgac agcgtgcggg tgatgacgta
     2941 gagcagcccg acccggtccg gacacttcac ctccaccacc gtgcggctgt ccgagatctg
     3001 gttgtccacc gagatcttgg gcgggccggc cacgccctcg ccactcgggt acaccgcccg
     3061 acgccgcgcg aggagatcct ccacgctcac ctcgccgcgc agcacgcgcc gcagcgcctc
     3121 gagggtgcgg cgccagcggg cctcctcggt gaccgcctcg ccgaagggat cgttcacctg
     3181 gaaggtgtcc agcacgatgc cgtcggcgcg cgtgtggatc tgggcggaga ggatgttgat
     3241 cccctgggag gccagcgtcc ccgcgatgag cgagaagagg ccgggcacgt cccgggtggc
     3301 gacgaccagc tcggaggagc cgaggtcggg atggtggaag agctcggtgg cgagcacgcc
     3361 gccgtcggtg agctggtcga gcagccgcag atgcgcggcg atgcgctggg gcgacgtggt
     3421 ggcgaggtag cgatcggaga gcagagccag gtgcacggcg accgcggtgc gcgccacctc
     3481 gccgcgcacg gccgcggtca cccgctgggc cacgctctcg cgagtgggcc gctcccgctg
     3541 cccgccggtg aggcgcgcga gcgtgcgcgc gtagagatcc cagaggatct gggcctgcca
     3601 gccggtcatg acaccggggc ccaccgcgcg catgtcggcg caggtgagca ggtagagcat
     3661 gcgcaggcgc tcgggcgtcc tgcagacctc ggcgagcgtc tcgatggtct tcgggtcgtc
     3721 gatgtcgcgg cgctgcgcga cgtgcgacat ggtcaggtgg tgggccacca ggaagacgac
     3781 cttgtccgcg tcctcggcgg gcaggccgat tcgcgcggag agcgcctcga tcagcgggat
     3841 gcccttggcc acgtggccgt gccccttgcc cttgccgatg tcgtgcagca gcatgcccag
     3901 catcagcagg cccgggcgct ccagctcgct caccacctgc gcgttgccct cggagtccgg
//
LOCUS       DASUFA010000136         5658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_144219, whole genome shotgun sequence.
ACCESSION   DASUFA010000136 DASUFA010000000
VERSION     DASUFA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144219"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFX87_04970"
     CDS             complement(<1..418)
                     /locus_tag="VFX87_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994295.1"
                     /translation="MRHRTRTVPLLLLLFAVVIALPVIPAASADADRGDWVARIADMD
                     RALAHGETTAATAAWREAYVAAHVSRGWPGMIAVGDAAVRAGRATGALDVFEPRARRA
                     YRTALLRAHRQASREGVLAAGEAFGRLGDRDVERLAR"
     gene            complement(592..1047)
                     /locus_tag="VFX87_04975"
     CDS             complement(592..1047)
                     /locus_tag="VFX87_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4994296.1"
                     /translation="MARHPRWVRRFLAEADLDAVATAIASAEAATSAEIRVHLDARCP
                     GEALARAALVFERLGMHRTAARHGVLIYVSIEDHKLAVLGDRGIHARVGQAYWDGLVE
                     SVLAHFRGQRFRDGLVHAVGEVGAVLRQHFPRRPDDVNELPDRVSVEPS"
     gene            complement(1047..1811)
                     /locus_tag="VFX87_04980"
     CDS             complement(1047..1811)
                     /locus_tag="VFX87_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4994297.1"
                     /translation="MLRRAALATLLLVLLAAPGAAALPVPPPPDRRVNDYAGALTPAD
                     RDRLEQQLAAREATSRNQVVVAVFRSLEGESLEGYSIRLAQAWRIGQKGLDNGVIFLV
                     FLDDRKMRIEVGYGLEGSLTDAVSSSILRDVVAPRFREGRTADGIAAGLDAIDRAIAG
                     TYARPPSADQGGRSGGLGARELGALFVVFVLIAILLQNHMQRAGVRRRGWTGGPTGWG
                     GPFIGGGFGGGSGGSSGGGFSGGGGGFGGGGSSGSW"
     gene            complement(1838..2431)
                     /locus_tag="VFX87_04985"
     CDS             complement(1838..2431)
                     /locus_tag="VFX87_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4994298.1"
                     /translation="MKTGLIVLGVIVLIVVGGIGWAIGINNQLVAQEQAVNEKWAQVQ
                     NVYQRRADLIPNLVETVKGYAAQERTVLEEVTKARASVAGIKATPELVNDPEAFRKFQ
                     AAQQELSGALARLLVTVERYPDLKSNQNFLTLQSQLEGTENRIAVERMRYSEAVRDYN
                     TRLRLFPGSLVASLRGFKEKAYFEAAPGSQDAPKVKF"
     gene            complement(2452..4029)
                     /locus_tag="VFX87_04990"
     CDS             complement(2452..4029)
                     /locus_tag="VFX87_04990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX4994299.1"
                     /translation="MTRLSQLMSVLAEKHADRVADVRLVRGNELHCRVPSSEIPALAA
                     LLRMGFGAELVFMAAGDRRADRGVFEVAYLFAPERENWLVHATAAVPAAAPAIVSLAT
                     FHYPASRFEREIYDLFGIVAEGHPDPRPLVRHAFWPEEYFPLRKDATAREFTDDGRAF
                     PFTEVGGEGVYEIPVGPVHAGVIEPGHFRFSVVGETIIDMKSRLYFTHKGTEKLFEGR
                     EPAAGVELAERVSGDTTIGHSLAFCQALEAAAGTAVPDRARWLRVILLEMERLYNHVA
                     DFGMIANDTGYAVAHSHCFRIRERLLRLNRRLTGNRLLRGGVVPGGVGHDLPADLDLP
                     GELETALRDFDEIVGLTLENTLVMDRFEGTGQLTTRIARDHGVLGYVARASGIDADVR
                     RDHPFAAYGDLEVRVPAFESGDVKARTLVRVEEAREAVRLVRAAVTRLPAGPLAAPLG
                     PLPPFEPAFGLVEGWRGAVIHWVMADAAGRLHRVKIMDPSFLNWRPLSYALLKNIVPD
                     FPLCNKSFNQSYSGNDL"
     gene            complement(4026..5483)
                     /locus_tag="VFX87_04995"
     CDS             complement(4026..5483)
                     /locus_tag="VFX87_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX4994300.1"
                     /translation="MTTFVMLLGPPLVAALLALVVRPYRRFVGWVNALLSLSSLGAAL
                     VLWSHLLAGRVLTSGPGEFLRADALSTLLAFCVSVVAALAAWLGPGMSRDDGYTGAEA
                     RRFRIFANLCAFAMLFAVTSNNVGFMWIAIEATTITSAMLIPLHVTKASVEASWKYIL
                     IGSVGIALAFGGTVLGYFDFVSLAGRQEAALNWTVLMASAPHLHPEVIQLAFVFILVG
                     YGTKAGLAPMHTWLPDAHSEAPAALSAVMSGVMLAVALYAVIRWEAVVNASAGTGFTD
                     GLYIALGVLSIAIASFSLVIQRNYKRMLAYSSIEHTGLICVGLALGPLGTFAAMLHLL
                     NHTLAKSMMFFLAGRVLHRYGTTEIGRVSGLLKVMPWTGGLFAVGVLAVIGLPPFGLF
                     ISEFALFRAGFAAGRPWLMGLVLALLAVAFVSMIGHLNRMLYGAPTAGVAVGEGNRWP
                     LVPLAICVTALLVLGITLPPPLQTLLRQIVEIVGP"
BASE COUNT          914 a         2012 c         2009 g          723 t
ORIGIN      
        1 ccctggccag ccgctcgaca tcccggtcgc ccaggcgacc gaaggcctcg cccgccgcga
       61 gcacgccctc gcgcgaggcc tgccggtgcg cccggagcag ggcggtgcgg taggcgcgcc
      121 gcgcgcgggg ctcgaagacg tcgagcgctc cggtggcgcg gcccgctcgc acggccgcgt
      181 cgccgaccgc gatcatgccc ggccagcccc ggctcacgtg cgcggccacg taggcttcgc
      241 gccacgccgc ggtggcggcc gtcgtctcgc catgcgcgag cgcccgatcc atgtcggcga
      301 tccgggccac ccagtcgccg cgatcggcgt cggccgatgc cgccgggatc acgggtagcg
      361 cgatgactac ggcgaacagg agcagcagga gcggtaccgt ccgcgtgcga tgtctcatgg
      421 cggcctcctc gctgaggtga agcatccgga cgaggggcga gcaccgcctg tgccaacggc
      481 gaagcccttg aaaatattgc gcgtatggag ggtacggtgc ccgcgcgtgg ccgggcacga
      541 tgcccggttg cggcagaatg cctgcccggc tcgggcgccg agaccgggcg atcaggacgg
      601 ctcgacgctc acccggtcgg gcagctcgtt gacgtcgtcg ggccgacgcg ggaaatgctg
      661 ccgcagcacc gcgcccacct cgccgacggc gtgcacgagg ccgtcgcgga accgctgccc
      721 gcggaagtgc gcgaggaccg actcgaccag cccatcccag taggcctggc ccacccgcgc
      781 gtggatgccg cggtcgccca gcaccgcgag cttgtgatcc tcgatgctca cgtagatcag
      841 caccccgtgg cgcgcggcgg tccggtgcat gccgagccgc tcgaagacga gcgcggcccg
      901 cgccagcgcc tctcccggac agcgcgcgtc gagatgcacc cggatctccg cggaggtcgc
      961 ggcctccgcg gacgcgatgg ccgtggccac cgcgtccaga tcggcctcgg ccaggaatcg
     1021 ccgcacccag cgtggatgac gcgccatcac cagctcccgc tcgagccgcc gccgccgaag
     1081 ccgccgccac ccccgctgaa ccctcccccg ctgctgccgc cgctcccgcc gccgaagccg
     1141 ccgccgatga acggcccgcc ccagcccgtc gggccgcccg tccacccgcg ccggcgcacc
     1201 cccgcgcgct gcatgtggtt ctgcaggagg atggcgatca gcacgaacac gacgaacagc
     1261 gcgccaagct ctcgcgcgcc cagcccaccc gagcggccgc cctggtccgc cgacggcggg
     1321 cgcgcatagg tgccggcgat cgcgcggtcg atggcgtcca ggcccgccgc gatgccgtcc
     1381 gcggtgcgtc cctcccggaa gcgcggggcg accacgtcac ggagaatgga cgacgagacc
     1441 gcgtcggtga ggctgccctc gagcccgtag ccgacctcga tgcgcatctt ccggtcgtcg
     1501 aggaagacga ggaagatcac gccgttgtcg aggcccttct ggccgatccg ccaggcctgg
     1561 gccagccgga tcgagtagcc ctcgaggctc tcgccctcga gcgagcggaa gacggcgacc
     1621 accacctggt tccggctggt ggcttcccgc gcggcgagct gctgctcgag acgatcccga
     1681 tcggcggggg tgagcgcgcc cgcgtagtcg ttgacgcgcc ggtcgggcgg cggcgggacg
     1741 ggaagcgccg ccgcgcccgg cgcggccagg agcaccagga gcagcgtggc gagcgccgcg
     1801 cgacggagca ccggctcgct cccgcccgaa gcgcccgcta gaacttcacc ttgggcgcgt
     1861 cctggctgcc cggcgctgct tcgaagtagg ccttctcctt gaacccgcgg agcgaggcca
     1921 ccaggctgcc cgggaagagc cgcaggcggg tgttgtagtc gcgcaccgct tcgctgtagc
     1981 gcatgcgctc caccgcgatg cggttctcgg tcccctcgag ctggctctgg agcgtcagga
     2041 agttctggtt ggacttgaga tcgggatagc gttcgacggt gaccaggagc cgcgcgagcg
     2101 cgccgctcag ctcctgctgg gccgcctgga acttgcggaa cgcctcggga tcgttcacca
     2161 gctcgggtgt ggccttgata ccggccacgc tggcgcgcgc cttggtcact tcctcgagca
     2221 cggtccgctc ctgcgccgcg tagcccttga cggtctcgac caggttcggg atgaggtcgg
     2281 cgcggcgctg gtagacgttc tgcacctgcg cccacttctc gttgaccgcc tgttcctggg
     2341 ccacgagctg gttgttgatg ccgatggccc agccgatgcc gccgaccacg atgaggacga
     2401 tcacgcccag aacgatcagt ccggttttca tgaacccagt atacccctcg gtcagagatc
     2461 gttgcccgag tacgactggt tgaacgactt gttgcagagg gggaagtcgg gaacgatgtt
     2521 cttcagcagc gcgtaggaca gcggccgcca gttcaggaat gacgggtcca tgatcttgac
     2581 gcgatggagt cgaccggccg cgtccgccat cacccagtga atgacggcgc cccgccagcc
     2641 ctcgaccagc ccgaaagcgg gctcgaacgg gggcagcggg ccgagcggcg ccgcgagcgg
     2701 gcccgcgggc agtcgcgtca ccgccgcccg gacgaggcgc accgcttccc gcgcctcctc
     2761 gacccgcacc agagtccgcg ccttgacgtc gcccgactcg aaggccggca cccgcacctc
     2821 gagatccccg taggccgcga agggatggtc gcgccggacg tcggcgtcga tgccggaggc
     2881 gcgcgccacg tagcccagca cgccgtggtc ccgcgcgatc cgcgtggtca gctggccggt
     2941 gccttcgaag cggtccatca cgagcgtgtt ctccagcgtg agcccgacga tctcgtcgaa
     3001 gtcccggagg gcggtctcca gctcaccggg cagatccagg tcggccggca gatcgtggcc
     3061 gacgccgccc gggacgacgc cgccccgaag cagccggttg ccggtgaggc gccggttgag
     3121 ccgcagcagc cgctcccgga tccggaagca gtgggagtgg gccaccgcgt agccggtgtc
     3181 gttggcgatc atgccgaagt cggccacgtg gttgtagagc cgctccatct ccagcaggat
     3241 cacacgcagc cagcgcgcgc ggtccggcac cgcggtgccc gcagccgcct cgagcgcctg
     3301 gcagaaggcg agcgagtggc cgatcgtggt gtccccggac acgcgctcgg ccagctcgac
     3361 gcccgcggcc ggctcgcggc cctcgaagag cttctcggtc cccttgtggg tgaagtacag
     3421 gcgcgacttc atgtcgatga tcgtctcgcc caccacgctg aagcggaagt ggccgggctc
     3481 gatcaccccg gcgtgcaccg ggcccaccgg gatctcgtac accccctcgc cgcccacctc
     3541 ggtgaacggg aaggcgcgcc cgtcgtcggt gaactcgcgc gccgtcgcat ctttccgaag
     3601 cgggaaatac tcctcgggcc agaaggcgtg gcgcacgagc ggccggggat cggggtggcc
     3661 ctcggccacg atcccgaaaa gatcgtagat ctcccgctcg aagcgcgagg ccgggtaatg
     3721 gaaggtggcc agcgagacga tcgcgggagc ggcggcggga accgcggcgg tggcatggac
     3781 gagccagttc tcccgctccg gggcgaacag gtaggcgacc tcgaacaccc cgcggtcggc
     3841 ccggcggtcg ccggcggcca tgaagaccag ctccgcgccg aagcccatgc ggaggagcgc
     3901 ggccagcgcg gggatttcgg acgaggggac tctgcagtga agctcgttgc cccgcaccag
     3961 gcggacgtcg gccactcgat cggcgtgctt ctcggcgaga acgctcatga gctgggacag
     4021 gcgggtcatg ggccgacgat ctccacgatc tggcgcagca gcgtctggag cggcggaggg
     4081 agcgtgatgc cgaggacgag gagggccgtc acgcagatgg cgagcgggac cagcggccat
     4141 cggttcccct cgcccaccgc cacgcccgcg gtgggcgcgc cgtagagcat ccggttgagg
     4201 tggccgatca tcgagacgaa cgcgaccgcg agcagggcca ggaccaggcc catcagccac
     4261 ggccggcccg cggcgaagcc ggcccggaag agcgcgaact ccgagatgaa gagaccgaag
     4321 gggggcaggc cgatcacggc caggaccccc accgcgaaca ggccgcccgt ccacggcatg
     4381 accttgagga gaccggagac ccggccgatc tcggtggtgc cgtaccggtg gagcacccgt
     4441 ccggccagga agaacatcat cgacttcgcc agcgtgtggt tgagcaggtg cagcatcgcg
     4501 gcgaaggtcc cgagcgggcc cagggccagc cccacgcaaa tcagcccggt gtgctcgatg
     4561 ctggagtacg cgagcatgcg cttgtagttg cgctggatca ccaggctgaa cgacgcgatc
     4621 gcgatcgaca ggacgccgag ggcgatgtag aggccgtccg tgaatccggt ccccgccgac
     4681 gcgttcacga ccgcctccca ccggatcacc gcatagagcg cgaccgcgag catcacgccg
     4741 gacatgacgg cggagagggc cgccggcgcc tccgaatggg cgtccgggag ccacgtgtgc
     4801 atgggggcga gaccggcctt ggtgccgtac ccgacgagga tgaagacgaa ggcgagctgg
     4861 atgacctcgg ggtggaggtg gggcgccgac gccatcagca ccgtccagtt cagcgccgcc
     4921 tcctggcggc cggccaggct gacgaagtcg aagtagccga ggacggttcc cccgaaggcg
     4981 agggcgatgc cgaccgagcc gatcaggatg tacttccacg acgcctcgac cgaggccttc
     5041 gtcacgtgga gcgggatgag catggccgac gtgatcgtgg tcgcctcgat cgcgatccac
     5101 atgaagccca cgttgttcga ggtcaccgcg aacagcatgg cgaaggcgca caggttggcg
     5161 aagatgcgga accgccgggc ctcggcgccg gtgtagccgt cgtcccggct catccccggc
     5221 ccgagccacg cggccagcgc cgccaccacg ctcacacaga acgcgagcag ggtcgagagc
     5281 gcgtcggcgc gcaagaactc cccgggcccc gaggtcagca cccggcccgc gagcaggtgg
     5341 ctccacagga ccagcgccgc gccgagcgag gaaagcgaca gcagcgcgtt cacccagccg
     5401 acgaagcgcc ggtagggtct caccaccagc gcgagcagcg ccgcgacgag aggcggaccg
     5461 agcagcatga cgaaggtagt cacgatccct cagtgccgga ggcggttcaa ctgctccacg
     5521 tcgatgctct cgaaggtctc gtggatctgg tagatgaaga tctgcatgac gaggaagccc
     5581 atgaggacgt ccaggaacac cccgagctcg acgatcaagg ggatgccgta ggtccccagg
     5641 accgcaagca gggcgagg
//
LOCUS       DASUFA010000137         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1277509, whole genome shotgun sequence.
ACCESSION   DASUFA010000137 DASUFA010000000
VERSION     DASUFA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277509"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFX87_05000"
     CDS             complement(<1..872)
                     /locus_tag="VFX87_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX4994301.1"
                     /translation="MIDFRSGREPKSSSFDYISIRIASPEEIRGPRDAKERERMELQG
                     LRSWWSWGEVTKPETINYRSFKPEKDGLFCERIFGPVKDWECHCGKYKRIRYRGVICD
                     RCGVEVTLSKVRRERMGHIELSVPVAHIWFFKTLPSPMGNMLGITLRELERVIYYSSY
                     IVLEPGKQEVTRLQLLDEEEFLTLRAKAREEGDGAFEADIGAPAVRTLLQQLDVKAIA
                     EQLRTGVANERSQHRKKQMLKRLKIVDAFLNSGAAGDGPNRPEWMILDVIPVIPPDLR
                     PLVPLDGGRFATSDL"
     gene            complement(883..>3765)
                     /gene="rpoB"
                     /locus_tag="VFX87_05005"
     CDS             complement(883..>3765)
                     /gene="rpoB"
                     /locus_tag="VFX87_05005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX4994302.1"
                     /translation="SQFMDQTNPLAELTNKRRLSALGPGGLTRERAGFEVRDVHYSQY
                     GRMCPIETPEGPNIGLITSLSTFARINDLGFIETPYRVVKEGRVTNEIVWLSASDEED
                     FAVAQANAELNPDGTFRDPLVLTRKGTDYPLLPPARIDYMDVAPEQLVSIAAALIPFL
                     EHDDANRARMGSNMQRQAVPLLFPRAPVVGTGLEGKVARDSGAVIVARRAGTVTRVTA
                     DEIIVDAGEEGRASDAPLGRLSQHDRYRVKKFWRTNQDTAINQRPLVQVGDRVGAGQV
                     IADGPGTDHGELALGSNVLVAFMPWYGHNFEDAIVLSERLVKDDVYSSIHIQELELHV
                     RDTKRGQEEITREIPNVSDEALVDLDERGIVRIGAHVKPGDILVGKITPKGETELSPE
                     EKLLTAIFGEKAKDVKDSSLKVPPGMKGVVIDVKIFSRVEDQVVEKDRGERIGEVRRV
                     ETEEKARITSVMIEELRDLLANQEVALMLKAGTVEEYLPAGSKLGRAELDTLDFGEVD
                     LKTLRVASKSANERLRAVIEAATRERARVEEKAEDQIDKILQPDELPPGVIQLVKVYC
                     AEKRKVAVGDKMAGRHGNKGIVARIVPEEDMPFLPDGTPVDICLNPLGVPSRMNVGQI
                     LETHLGWCARILGFEAKTPVFRGADEAEIGLLLRLVGLEWAAQSLRLPGAPGLDAGAV
                     PALITDLHATAPRGDATPMHEAHVDLLGAKGASAASKALHQRLRGFLATAAETLAARE
                     ADQQRLAATANTALAEASEGAGRKAAQKAAKELEKAAGRSPAEHLEAAGMPALARVLG
                     AKDAPLDPAAVELLRAAGLTPAGKARLRDGRTGREFASDVTVGSIYMLKLSHLVDDKI
                     HARSIGPYSLVTQQPLAGKAQFGGQRFGEMEVWALEAYGAAHVLQEMLTVKSDDVNGR
                     SRVYEAIVKGQNLPEPGIPESFNVLVKELQALGLKVTLGTTAEADE"
BASE COUNT          470 a         1356 c         1315 g          624 t
ORIGIN      
        1 aggtcggacg tggcgaagcg cccgccgtcg agcggcacca gcggccgcag gtcgggcggg
       61 atgaccggga tgacgtccag gatcatccac tccggccggt tggggccgtc gcccgcggcg
      121 ccggagttga ggaacgcgtc cacgatcttg agccgcttga gcatctgctt cttgcgatgc
      181 tgggagcgct cgttggcgac cccggtgcgg agctgctcgg cgatcgcctt cacgtccagc
      241 tgctggagca gggtgcgcac cgccggggcg ccgatgtcgg cctcgaaggc gccgtcgccc
      301 tcctcgcgcg ccttggcgcg gagggtgagg aactcctcct cgtccagcag ctggaggcgg
      361 gtcacctcct gcttgcccgg ctcgagcacg atgtagctcg agtagtagat cacccgctcc
      421 agctcgcgga gcgtgatccc gagcatgttc cccatcgggg acggcagggt cttgaagaac
      481 cagatgtgcg ccaccggcac gctgagctcg atgtggccca tccgctcgcg gcggaccttg
      541 ctcagcgtca cctccacccc gcagcggtcg cagatgaccc cgcggtagcg gatgcgcttg
      601 tacttgccgc aatggcactc ccagtccttg accggaccga agatccgctc gcagaacagg
      661 ccgtccttct ccggcttgaa ggagcggtaa ttgatggtct ccggcttcgt cacctcgccc
      721 caggaccacc acgaacggag gccctgcagc tccatccgct cgcgttcctt ggcgtcccgc
      781 gggccccgga tctcctcggg gctcgcgatg cggatggaga tgtagtcgaa gctcgagctc
      841 ttcggctcgc ggccgctgcg aaaatcgatc atgttccgcc ccttactcgt ccgcctcggc
      901 ggtcgtgccg agggtgacct tgagaccaag cgcctggagt tccttgacca gcacgttgaa
      961 cgactccggg atgcccggct cggggagatt ctgccccttc acgatggcct cgtacacccg
     1021 gctccggccg ttgacgtcgt ccgacttgac cgtgagcatc tcctgcagca cgtgcgcggc
     1081 gccgtacgcc tccagcgccc acacctccat ctcgccgaac cgctggccgc cgaactgcgc
     1141 cttgccggcg agcggctgct gggtgaccag cgagtacggg ccgatcgagc gcgcgtggat
     1201 cttgtcgtcc accaggtggc tcagcttgag catgtagatc gagcccaccg tcacgtcgct
     1261 cgcgaactcc cgcccggtgc ggccgtcgcg gagccgcgcc ttgcccgccg gggtgaggcc
     1321 cgccgcccgc agcagctcca ccgccgcggg gtcgagcggg gcgtccttgg ccccgagcac
     1381 ccgcgcgagg gccggcatcc ccgccgcctc gaggtgctcc gccgggctcc gcccggcggc
     1441 cttctccagc tccttcgcgg ccttctgggc cgccttccgc ccggccccct ccgacgcctc
     1501 ggcgagggcg gtgttggcgg tcgcggccag ccgctgctgg tcggcctcgc gcgcggcgag
     1561 cgtctccgcc gcggtcgcga ggaacccgcg gagccgctgg tgcagcgcct tcgaggccgc
     1621 cgaggcgccc ttcgcgccca gcaggtccac gtgcgcctcg tgcatcgggg tcgcgtcgcc
     1681 gcgcggcgcg gtggcgtgca ggtcggtgat gagcgccggc acggcgccgg cgtccagccc
     1741 cggcgcgccg gggaggcgga gcgactgcgc cgcccactcg agccccacca gccggagcag
     1801 caggccgatc tccgcctcgt cggcgccgcg gaacaccggc gtcttggcct cgaagccgag
     1861 gatccgcgcg caccacccga ggtgggtctc gaggatctgc ccgacgttca tccggctcgg
     1921 cacgccgagc gggttgaggc agatgtccac cggggtgccg tccgggagga acggcatgtc
     1981 ctcctcgggg acgatccggg cgacgatgcc cttgttgccg tggcggccgg ccatcttgtc
     2041 gccgacggcc accttccgct tctcggcgca gtacaccttg accagctgga tgacgcccgg
     2101 gggcagctcg tccggctgga ggatcttgtc gatctggtcc tcggccttct cctcgacccg
     2161 ggcgcgctcg cgcgtggccg cctcgatgac ggcgcggagc cgctcgttgg ccgacttgct
     2221 ggccacgcgg agggtcttga ggtccacctc gccgaagtcc agggtgtcga gctcggcccg
     2281 gccgagcttg ctgccggcgg ggaggtactc ctccaccgtg ccggccttga gcatcagcgc
     2341 cacctcctgg ttggccagga ggtcgcgcag ctcctcgatc atcacgctgg tgatgcgggc
     2401 cttctcctcg gtctcgaccc ggcgcacctc gccgatccgc tcgccgcggt ccttctcgac
     2461 cacctggtcc tcgacgcggc tgaagatctt cacgtcgatg accacgccct tcatcccggg
     2521 cggcaccttg agcgaggagt ccttcacgtc cttggccttc tcgccgaaga tggccgtgag
     2581 gagcttctcc tcgggggaga gctcggtctc gcccttcggg gtgatcttgc cgaccaggat
     2641 gtcgcccggc ttcacgtggg cgccgatccg cacgatgccc cgctcgtcga ggtccaccag
     2701 cgcctcgtcg gagacgttgg ggatctcgcg ggtgatctcc tcctggccgc gcttggtgtc
     2761 gcggacgtgg agctcgagct cctggatgtg gatcgacgag tagacgtcgt ccttcaccag
     2821 ccgctcggag aggacgatgg cgtcctcgaa gttgtgcccg taccacggca tgaacgcgac
     2881 cagcacgttg ctgccgaggg ccagctcccc gtggtcggtg ccggggccgt cggcgatgac
     2941 ctgccccgcc ccgacccggt cacccacctg gaccagcggg cgctggttga tggcggtgtc
     3001 ctggttggtg cgccagaact tcttgacccg gtagcggtcg tgctggctga gccggccgag
     3061 cggcgcgtcg gaggcgcggc cctcctcgcc ggcgtccacg atgatctcgt cggccgtcac
     3121 ccgggtgacg gtgccggcgc ggcgggcgac gatcacggcg cccgagtcgc gcgccacctt
     3181 gccctcgagc ccggtgccga ccaccggcgc gcgggggaag aggagcggca cggcctggcg
     3241 ctgcatgttc gagcccatcc gcgcgcggtt cgcgtcgtcg tgctccagga acgggatcag
     3301 cgccgccgcg atggagacca gctgctccgg cgccacgtcc atgtagtcga tccgggccgg
     3361 cgggagcagc gggtagtcgg tgcccttccg ggtcagcacc agcgggtcgc ggaaggtgcc
     3421 gtccgggttg agctcggcgt tggcctgggc gacggcgaag tcctcctcgt cgctggccga
     3481 gagccagacg atctcgttgg tgacccggcc ctccttcacc acgcggtagg gcgtctcgat
     3541 gaagccgagg tcgttgatcc gcgcgaaggt cgagaggctc gtgatcaggc cgatgttcgg
     3601 gccctcgggc gtctcgatcg ggcacatccg gccgtactgc gagtagtgga cgtcgcggac
     3661 ctcgaacccg gcccgctcgc gggtcaggcc gccgggcccg agcgcggaga ggcggcgctt
     3721 gttggtcagc tccgccagcg ggttggtctg gtccatgaac tgcga
//
LOCUS       DASUFA010000138         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1400265, whole genome shotgun sequence.
ACCESSION   DASUFA010000138 DASUFA010000000
VERSION     DASUFA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400265"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1023
                     /locus_tag="VFX87_05010"
     CDS             67..1023
                     /locus_tag="VFX87_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4994303.1"
                     /translation="MKIAVVAERVREVVTTLGRIVVGKDDVLERILAGVLANGHVLVE
                     DYPGLAKTLIARLLAQALDLGFRRIQFTPDLLPSDITGSFLYDQREARFEFRAGPVFT
                     NLLLADEINRATPKTQAALLEAMQEAQVTIEGQSFPLEQPFVVIATQNPIEMEGTYPL
                     PEAQLDRFLLRVGIGYPDAAAEREILKRRRERRTDAATVPTIVTRVELLAMQMALEDV
                     FVSEALERYIVALSQATRTDPRVTLGASPRGSLALLKLARAEAAIRGRDFVLPDDVKA
                     VAVAALAHRLVLRPELWVSSTTPRHVVEGVLREVPAPAGEPA"
     gene            1020..2297
                     /locus_tag="VFX87_05015"
     CDS             1020..2297
                     /locus_tag="VFX87_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4994304.1"
                     /translation="MTWRPTALNISFLTVVGWALFLTVVTGRVELAVVAVPAIVALVA
                     GRRPGAPPAWRIHRELSTARLLEDECVTVTVTLRGEGALPLVELLEPLPPRVGLERGS
                     NHAFFTLPAGREIRWTFELRGVGRQHLRLEGPYVRVWGLLGLAAAEARHRAPVTAAVY
                     PRHAPIRYLPHPRRTQTFVGNYVSPTQGAGIEPGDIRPFAPGDQIRHVNWRATLKLGA
                     LHVTRHHQERNADVVLLLDTLSEVGPDGETVVDAAARGAAALAAAYLARKDRVGLIEY
                     GGQPRWVRPGAGRAHLERLLDTLLSASVVFTYVNRDLDLVPPRILPPQALVVALSPLL
                     DRRFVRAATDLAARGFDLLVIAVSPVPAARAAARRSAVADVAARLWALERRAELEEYR
                     RHGLTIVEWDGAEPLDAVLAAWSRRSRRRVLAG"
     gene            2294..>2632
                     /locus_tag="VFX87_05020"
     CDS             2294..>2632
                     /locus_tag="VFX87_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994305.1"
                     /translation="MMRLAAMVLLAVVAALPLGVRPSTPPVTWLAVAALAVGGVGVVA
                     WSVPVVTAAGSLVVIAYALALAIAGAADDSLTAIVLGAALVLLLALVHFGGRVRGAMM
                     GPSVIASQARQ"
BASE COUNT          300 a          967 c          980 g          385 t
ORIGIN      
        1 ccggctcggc cgtggaccca gcctgggcga tcttcgccgc gtgatcgcgg agatcgagag
       61 cgagcggtga agatcgcggt cgtcgccgag cgggtgcgcg aggtggtcac gacgctcggc
      121 cggatcgtgg tgggcaagga cgacgtgctc gagcggatcc tggccggcgt cctcgccaac
      181 ggccacgtgc tcgtcgagga ctacccgggc ctcgccaaga ccctgatcgc ccggctcctg
      241 gcccaggcgc tcgacctggg cttccggcgc atccagttca cacccgacct gctgccgagc
      301 gacatcaccg gcagcttcct gtacgaccag cgcgaagccc ggttcgagtt ccgggccggc
      361 ccggtcttca cgaaccttct gctcgccgac gagatcaatc gcgccacgcc caagacgcag
      421 gcggcgttgc tcgaggccat gcaggaagcg caggtcacga tcgagggcca gtcgttcccg
      481 ctcgagcagc cgttcgtcgt gatcgccacc cagaacccga tcgagatgga gggcacctac
      541 ccgctgccgg aggcccagct cgaccggttc ctgctgcgcg tgggcatcgg gtacccggat
      601 gcggccgccg agcgggagat cctgaagcgg cgccgcgagc gccgcaccga cgccgccacc
      661 gttccgacca tcgtgacccg ggtggagctc ctggccatgc agatggcgct cgaggacgtc
      721 ttcgtgagcg aggcgctcga gcgctacatc gtggctctct cgcaggccac gcgaaccgac
      781 ccgcgggtga cgctgggggc ctcgccgcgg ggcagcctgg ccctgctcaa gctggcccgg
      841 gcggaagcgg ccatccgcgg gcgcgacttc gtgctgcccg acgacgtgaa ggcggtggcg
      901 gtggccgccc tggcccaccg gctcgtcctc cgaccggagc tgtgggtctc gagcacgacc
      961 ccgcgccacg tggtggaagg cgtgctccgg gaggttcccg cgccggccgg agagcccgcg
     1021 tgacctggcg gccgacggcg ctgaacatct ccttcctgac cgtggtcggc tgggcgctct
     1081 tcctgaccgt ggtgaccggg cgcgtcgagc tcgcggtggt ggcggtgccc gcgatcgtgg
     1141 ccctcgtggc cgggcggcgc cccggggcgc cgcccgcctg gcggatccat cgcgagctgt
     1201 ccaccgcgcg cctgctcgag gacgagtgcg tgaccgtcac cgtgacgctc cgcggggagg
     1261 gggcgctgcc gctcgtcgag ctcctggagc cgctgccgcc gcgggtcggg ctcgagcgcg
     1321 ggagcaacca cgcgttcttc acgctcccgg cgggccggga gattcgctgg accttcgagc
     1381 tgcgcggcgt cggccgccag cacctgcggc tcgaggggcc gtacgtgcgg gtgtggggac
     1441 tgctcgggct cgccgccgcc gaggcccggc atcgcgcgcc cgtgacggcg gccgtctacc
     1501 cacggcacgc cccgatcagg tacctgccgc acccgcggcg cacgcagacg ttcgtgggca
     1561 actacgtgtc tccgacgcag ggcgcaggca tcgagcccgg cgacatccgg cccttcgcgc
     1621 ccggcgatca gatccggcac gtgaactggc gcgccacgct gaagctgggc gcgctccacg
     1681 tgacgcggca tcaccaggag cggaacgccg acgtggtcct cctgctcgat accctgtccg
     1741 aggtgggccc ggacggcgag acggtcgtgg acgctgcggc ccggggcgcg gccgcgctcg
     1801 ccgcggcgta tctggcccgc aaggaccgcg tcggcctgat cgagtacggg ggccagcccc
     1861 gctgggtgag gccgggcgcg ggccgcgccc atctcgagcg cctgctcgat acgctgctga
     1921 gcgcgtcggt cgtcttcacg tacgtgaatc gcgacctcga tctggtgccg ccgcgcatcc
     1981 tgccgcccca ggccctggtg gtggcgctga gcccactgct cgaccggcgc ttcgtacggg
     2041 ccgcgaccga cctcgcggcg cggggcttcg atctcctggt catcgcggtc tcgccggttc
     2101 cggcggcccg cgccgcggca cgccgatccg cggtcgccga cgtggcggcc cggttgtggg
     2161 ccctggagcg ccgcgcggag ctcgaggagt accgccgcca cgggctgacc atcgtcgagt
     2221 gggacggcgc cgagcccctc gacgcggtcc tggctgcctg gagccgccgg tcgcgccgcc
     2281 gcgtgctggc cggatgatgc gcctggccgc gatggtgctg ctcgcagtcg tggctgcgct
     2341 tccgctcgga gtccggccgt cgacgccacc cgtcacctgg ctcgcggtgg ccgcgctcgc
     2401 ggtgggtggc gtcggggtgg tcgcgtggtc ggtgccggtc gtgaccgcgg ccggctcgct
     2461 ggtggtgatt gcctacgcgc tcgcgctcgc gatcgccggg gcagcggacg attccctcac
     2521 cgcgatcgtg ctcggcgcgg cgctcgtgct cttgctggcg ctcgtccact tcgggggccg
     2581 tgtccgcggg gccatgatgg gcccgtccgt gatcgcctcg caggcgcgcc ag
//
LOCUS       DASUFA010000139         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_834297, whole genome shotgun sequence.
ACCESSION   DASUFA010000139 DASUFA010000000
VERSION     DASUFA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834297"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2083)
                     /locus_tag="VFX87_05025"
     CDS             complement(<1..2083)
                     /locus_tag="VFX87_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4994306.1"
                     /translation="MTDRLQEQEPLHPVARGLAAPASGLERFPPPARWDDWEELDAKA
                     WPRRVKRRFQLIPTICFNCEAGCGLLAYVDPQTLQIQKFEGNPVHPGSRGRNCAKGPA
                     TLNQITDPERILHPLRRAGKRGEGKWERVTWDAVLDDIGGRIRKALVEGRQKEVMYHL
                     GRPGHELIYLQRVFHAWGIDGHNSHTNVCSASARAGYGFWHGLDRPSPDHSKARFILL
                     LSSHLEAGHYFNPHAQRIMDGKMAGAKICVVDTRLSNTASMADYWLSPWPGTEAALLL
                     AIVHCMLRDGTWDREFVRRWVNWEQYLREERPELPVTMASFEAALKTLYAEYTPEFAE
                     SESGVPAARIEDVAREVGRAGSALSTHVWRNAAAGNLHGWEVARALELVVVLAGAVAT
                     PGGTAPSGWNKAVPTPPMMPPPGKIWSELLMPREYPLAFFEMSFLLPHFLKEGRGKLA
                     MYFTRVYNPVWTNPDGMSWIEMLTDEAKVERHACLTPTWSETAWFADYVLPMGHASER
                     HDLMSQETHAARWIGFRQPVLRVALEKQGKRFDLTWQAHEAAGLGQVWEEDEFWIELS
                     WRIDPDGALGIRKYFESPYRPGEKLRIEEFYQWIFEHSVPGLPAAAERLGLTPLAYMR
                     KYGAFLVQESVYRTHETPVAAGEGVEVDGQWMGGFPTPSRKLEFYSRTLKEWKWPEHA
                     VPTYARSHVHWR"
     gene            complement(2123..>2609)
                     /gene="nrfD"
                     /locus_tag="VFX87_05030"
     CDS             complement(2123..>2609)
                     /gene="nrfD"
                     /locus_tag="VFX87_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX4994307.1"
                     /translation="PMALVAAATAGYSAFLFGQAEGRDFWQSPLLLPHLLVAALVGGS
                     ASLLLAARVVGSASFIVGGLGLVLWASLLVSALVLGAELFGAHGAEDARRAARLLTRG
                     PLRGPLWGGVAAAGIALPLVLLLSSSVPFGSELAAGLALAGLWLWEELWIRAGQMLPL
                     S"
BASE COUNT          382 a          967 c          892 g          368 t
ORIGIN      
        1 cccgccagtg cacgtggctg cgcgcgtagg tgggcaccgc gtgctccggc cacttccact
       61 ccttgagcgt cctcgagtag aactcgagct tgcgcgacgg cgtcgggaag ccgcccatcc
      121 actgcccgtc cacctcgacg ccctctcccg cggcgaccgg cgtctcgtgg gtgcggtaga
      181 cgctctcctg gaccaggaag gcgccgtact tgcgcatgta cgcgagcggc gtcaggccta
      241 ggcgctcggc ggccgcgggc agtcccggca cgctgtgctc gaagatccac tgatagaact
      301 cctcgatgcg cagcttctcg ccgggacgat agggcgactc gaagtacttg cggatgccga
      361 gcgcgccgtc cggatcgatg cgccacgaca gctcgatcca gaactcgtcc tcctcccaca
      421 cctgaccgag ccccgccgcc tcgtgggcct gccacgtgag atcgaagcgc ttcccctgct
      481 tctcgagcgc cacccgcagt accggctggc gaaagccgat ccagcgcgcg gcgtgcgtct
      541 cctggctcat gaggtcgtgg cgctcggagg cgtgccccat cggcagcacg tagtcggcga
      601 accacgcggt ctcgctccag gtcggggtga ggcaggcgtg gcgctcgacc ttcgcctcgt
      661 cggtcagcat ctcgatccac gacatgccgt ccgggttggt ccacaccggg ttgtagaccc
      721 gggtgaagta catcgcgagc ttgccgcgcc cttccttcag gaagtgcggc aggagaaagc
      781 tcatctcgaa gaaggccagc gggtactccc gcggcatcag gagctcgctc cagatcttgc
      841 cgggcggcgg catcatcggc ggggtcggca ccgccttgtt ccagccggag ggcgcggtgc
      901 cgcccggcgt ggccaccgcg cccgccagca ccaccaccag ctcgagcgcg cgcgccacct
      961 cccagccgtg gagattgccc gccgcggcgt tgcgccagac gtgggtggac agcgcgctgc
     1021 ccgcgcggcc gacctcgcgc gccacgtcct cgatgcgcgc ggcgggcacc cccgactcgc
     1081 tctccgcgaa ctcgggggtg tactcggcgt agagcgtctt gagcgcggcc tcgaacgacg
     1141 ccatcgtgac cggcagctcc ggccgctcct cacgcaggta ctgctcccag ttcacccagc
     1201 gccggacgaa ctcgcggtcc cacgtgccgt cgcgcagcat gcagtgcacg atggccagga
     1261 gcagcgcggc ctcggtgccc ggccagggcg agagccagta gtcggccatc gaggcggtat
     1321 tgctgaggcg cgtgtccacc acacagatct tggcccccgc catcttgccg tccatgatcc
     1381 gctgggcgtg cgggttgaag tagtggccgg cctcgagatg cgaggagagc agcaggatga
     1441 agcgcgcctt gctgtgatcg ggcgacggcc ggtcgagccc gtgccagaac ccgtagcccg
     1501 cgcgcgcgga ggccgagcag acgttggtgt ggctgttgtg gccgtcgatg ccccaggcgt
     1561 ggaagacgcg ctggagatag atcagctcgt ggcccggccg cccgaggtga tacatcactt
     1621 ccttctggcg gccctcgacc agcgccttcc ggatgcgccc gccgatgtcg tcgagcaccg
     1681 cgtcccaggt cacgcgctcc catttgccct cgccgcgctt cccggcccgc cgcagcggat
     1741 ggaggatgcg ctcggggtcc gtgatctggt tcagcgtcgc agggcccttg gcgcagttgc
     1801 gcccgcggct gcccgggtgc accgggttgc cctcgaactt ctggatctgc agcgtctgcg
     1861 gatcgacgta ggcgagcagc ccgcagcccg cctcgcagtt gaagcagatg gtcggaatga
     1921 gctggaaccg ccgcttgacc cgcctgggcc aggccttggc gtcgagctcc tcccagtcat
     1981 cccaccgcgc cggcggcgga aaccgctcga gcccggaagc cggcgccgcc agcccccgcg
     2041 ccaccggatg gaggggctcc tgctcctgca gtcgatcggt catggaggcg ttccgtcctc
     2101 tttcttgcgc gctcgagtgc ggttagctga gcgggagcat ctggcccgcg cggatccaga
     2161 gctcctccca tagccagagt ccggccagcg ccaggcccgc ggccagctcg gagccgaagg
     2221 gcaccgacga gctcagcagc agcaccagcg gcagcgcgat gcccgcggcc gccacgccgc
     2281 cccagagcgg cccgcgcagg ggcccgcggg tgagcagccg ggccgcccgc cgcgcgtcct
     2341 ccgcgccgtg cgcgccgaag agctcggcgc ccagcacgag cgcgctcacc agcagggagg
     2401 cccagagcac gagccccagg ccgcccacga tgaacgacgc gctgccaacc acacgcgcgg
     2461 cgagcaacag gctcgccgag ccgccgacga gcgcggccac cagcaggtgc ggcagcagca
     2521 gggggctctg ccagaagtcg cgcccctcgg cctggccgaa gagaaacgcg ctgtagcccg
     2581 cggtcgccgc cgccaccagg gccatgggc
//
LOCUS       DASUFA010000140         8096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_834323, whole genome shotgun sequence.
ACCESSION   DASUFA010000140 DASUFA010000000
VERSION     DASUFA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834323"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFX87_05035"
     CDS             <1..419
                     /locus_tag="VFX87_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994308.1"
                     /translation="APNGAVLIDTLARALKERGFSRPPGSPRLITRLRRIRQLVVSQT
                     GRITLADAGGAASKPADTGPMAETDLEPVEMELGELGASADEATPGNEAPGHEVSPPT
                     PETPHRPRRRRRRRGGRGRGRGRGGGGAAEPVPAPA"
     gene            506..1672
                     /locus_tag="VFX87_05040"
     CDS             506..1672
                     /locus_tag="VFX87_05040"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4994309.1"
                     /translation="MADFKSLDSTFSQAVAAKRIPGVVAMATTDKGVLYEGAFGQREI
                     GKPAPMTLDTVMWIASMTKAITATAAMQLVERGKLALERPAAEVVAELGAVRVLEGFD
                     AAGKPRLRAPKRAMTLRHLLTHTAGFSYEIWSPAIGQFQQATGTPGITTCTNAALGTP
                     LLFDPGDKWDYGINIDWVGKMVEAVSGQRLDRYLQDHILGPLGMKDTSFKLSVSQRER
                     LSAMHQRADDGSLAAIEFGLPQEPEFHMGGGGLYGTVPDYMAFCRMIMNGGTHNGTQL
                     LQKSTVDLMAQNHIGALEIGTLKTAIPPLSKDVELFPGMSKKWGLSFLINTQAVPGGR
                     SANSLAWAGLANTYFWIDRTKRVCGVFASQLLPFYDDVAIDLFGKLETSVYQNV"
     gene            1691..2746
                     /locus_tag="VFX87_05045"
     CDS             1691..2746
                     /locus_tag="VFX87_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HEX4994310.1"
                     /translation="MPGPGLSGQAQTVLGPIAGEAMGITLPHEHLLIDFEVMFREPAN
                     GAERGLARQPVGLANLGWVRHNFSSNLDNLQLLDEAVARDEALLFKHAGGQTFVDPTN
                     RGLARDPLALARVARATGLHIIMGSGYYVAAAHPPDMDRRTADDIARELITDLTDGVD
                     GTGVRAGFIGEIGTTWPWTDNEKKVVRAAVAAQRDTGAALMIHPGRHERLPLAIVDFI
                     RKEGADLGRTIMCHIERTIADPAVLLELAATGVRLEYDLFGLETSYYPYKPAFDMPND
                     GERMRQILFLIERGHLDQILMSHDIAYKHCLTRWGGFGYHHLLVNVIPRLRAKGADDK
                     IVQTLLIDNPRRAFVYA"
     gene            2769..4313
                     /locus_tag="VFX87_05050"
     CDS             2769..4313
                     /locus_tag="VFX87_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994311.1"
                     /translation="MSPRPIGPVVLGALLLLAALTPASAQAPAGPAGEIAVSFHVTLA
                     PSWFDPSTAPPQITPFGVLYAIHDALVRPLPGQKMAPSLAESWTESADGLVYEFKLRR
                     GLKFHNGDPVTTDDVKWSFERYQGAGAKEFRARMRQVEIVDPLVIRFHLKEPWPDFMT
                     FYGTTATAAAIVLPRKYTEQAGPDGFRQRPIGAGPYRFVSQRQGVDVTLEAFSGHWRK
                     TPFIKKLTMKSVPDSTTRLTMLKSGETDFALFLDGPEGEAVTKDPRMQLVATRHASIF
                     WVEFADQWDPKSPWSDRRLRLAVNHALDRKGTNEAACLGHCPPAGVIVPRVMDFALQV
                     PAPAYDPQKARQLLAEAGFPKGLDAGEFAAIPGFATVGEAALNNLNAVGIRMRMRPME
                     RAAFYAAWQEKKLRGLFLTAVGNSGNAASRVESFMYSKGNAAYGGYPDIDELFLQQAR
                     ERDPAKREILLHRIQQLTIDRVMFAPVMDLRGLIGVGPRVAEHTINSVPMYPFPSYED
                     VRLKNP"
     gene            4362..5378
                     /locus_tag="VFX87_05055"
     CDS             4362..5378
                     /locus_tag="VFX87_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994312.1"
                     /translation="MAEGHRAVSVLYQALLTGLVLTLVTRRTEDEAADFVFRLFRRQH
                     LEKFLPGLAKLGLDGLPPAVACAQYHYLSNQMGGVKTEYAYESDRKAWVRYPPPRWIW
                     YGSAICGIPSRVNAAILHGWHGHNGVTLGNPRLGFVCTGQTVDGHPGLEGYYVEHDRD
                     LAPDERVRFAPGEIMPDADPARAPRLDPAAWPAERLELVVSRYAMEYVRTALPVLLQL
                     LGPPDTRRVLGHAARLIGMQYHDETARLLGIADSGPAAFARYLAALAAAQGEEVEVST
                     EPGRARVRQKGWRLERGLEPLDPGAFHAWAELWHGALAAHDRRLRLTVTRETDAIDFD
                     IISR"
     gene            5390..6199
                     /locus_tag="VFX87_05060"
     CDS             5390..6199
                     /locus_tag="VFX87_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4994313.1"
                     /translation="MLCSVRGCRLPLAREGQRLTCPRAHSFDVARRGYVNLLQPQDRR
                     SARPGDSAAAVAARRRFLSRGFEATLTRAIGDLLTLTPADALLEVGCGEGHYLGAFTA
                     RFGCEGHGVDISVPAIEAAAARHAGLHLVVANADRALPYADASFAVVASITARRNPAE
                     FRRVLRDDGRLLLVIPGPDDLVELREAVLGRGLLRDRSDQAVAAFAPRFVLERHERIR
                     HMARLDAAAIHDVMTGSYRAGRASRRDRLAALGPLTVTLSRDALVLRPIRP"
     gene            complement(6200..8096)
                     /locus_tag="VFX87_05065"
                     /pseudo
     CDS             complement(6200..8096)
                     /locus_tag="VFX87_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017340122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
BASE COUNT         1163 a         3066 c         2706 g         1161 t
ORIGIN      
        1 acgcgcccaa cggcgcggtg ctcatcgaca cgctggcccg cgcgctcaag gagcgcggct
       61 tcagccggcc gccgggctcg ccccggctga tcacccgcct gcgccgcatt cgccagctcg
      121 tcgtgagcca gaccggccgc atcacgctgg ccgacgcggg aggcgccgcg tccaagccgg
      181 ccgacacggg cccgatggcc gagaccgacc tcgagcccgt cgagatggag ctcggcgagc
      241 tcggcgcttc ggccgacgag gccacgccgg gcaacgaggc cccgggccac gaggtctctc
      301 cgccaacacc cgagaccccg caccggcccc gccgccgccg ccgccgtcgc gggggacgtg
      361 gacggggacg ggggcgcggt ggtggcggcg cggccgagcc ggtacccgcc ccagcttgag
      421 cgcttgatcc cgggcggccc cggcggtagc gtcgggggcg cccgcacccg ggcctacccg
      481 caaccgcttc agcaaggagg ctgacatggc ggacttcaag agcctcgact cgacattcag
      541 ccaggccgtc gccgcgaagc gcatccccgg cgtggtggcc atggcgacca ccgacaaggg
      601 cgtgctctac gagggcgcct tcggccagcg cgagatcggc aagccggccc cgatgaccct
      661 cgataccgtg atgtggatcg cctcgatgac caaggcgatc accgcgactg ccgcgatgca
      721 gctcgtggag cgcggcaagc tcgccctgga gcggccggcc gcggaggtgg tggccgagct
      781 gggcgccgtc cgcgtgctcg agggcttcga cgccgccggc aagccgcggc tgcgggcgcc
      841 caagcgggcg atgacgctgc gccacctcct cacccacacc gccgggttct cgtacgagat
      901 ctggagcccg gccatcggcc agttccagca ggccaccggc acccccggca tcaccacctg
      961 cacgaacgcc gcgctcggca cgccgctgct cttcgatccc ggagacaagt gggactacgg
     1021 catcaacatc gactgggtcg gcaagatggt cgaggcggtg agcggccagc ggctcgaccg
     1081 ctacctccag gaccacatcc tcgggccgct cggcatgaag gacacctcct tcaagctctc
     1141 ggtctcccag cgtgagcgcc tctccgcgat gcaccagcgg gcggacgacg gcagcctcgc
     1201 cgcgatcgag ttcgggctcc cgcaggagcc ggagttccac atgggcgggg gcggcctcta
     1261 cggcaccgtc cccgactaca tggcgttctg ccgcatgatc atgaacgggg gcacgcacaa
     1321 cggcacgcag ctgctgcaga agagcaccgt ggatctcatg gcccagaacc acatcggcgc
     1381 cctcgagatc ggcacgctca agaccgcgat cccgccgctc tccaaggacg tggagctctt
     1441 cccggggatg agcaagaagt gggggttgag cttcctgatc aatacccagg cggtgcccgg
     1501 cggccgctcg gccaactccc tggcctgggc cgggctcgcc aacacctact tctggatcga
     1561 tcgcacgaag cgcgtgtgcg gcgtcttcgc ctcccagctc ctgccgttct atgacgacgt
     1621 cgcgatcgac ctgttcggga agctcgagac cagcgtgtac cagaacgtct aggcgcccct
     1681 gggccacggc atgccaggcc ccggcctctc gggacaggcc cagaccgtgc tcggtcccat
     1741 cgccggcgag gcgatgggaa tcaccctgcc ccacgagcat ctcctcatcg acttcgaggt
     1801 gatgttcagg gagcccgcca acggcgccga gcggggcctg gcccggcagc cggtgggcct
     1861 ggccaatctc ggctgggttc gccacaactt cagctccaac ctcgacaacc tccagctcct
     1921 cgacgaggcg gtggcccgcg acgaggcgct gctcttcaag cacgcgggcg gccagacctt
     1981 cgtggacccg acgaatcgcg ggctcgcgcg ggatcccctc gcgctcgccc gcgtcgcccg
     2041 cgccaccggc ctccacatca tcatgggctc cggctactac gtggccgccg cgcacccgcc
     2101 ggacatggac cggcgcaccg ccgacgacat cgcgcgcgag ctgatcacgg atctcaccga
     2161 cggcgtggac ggcaccgggg tgcgcgccgg cttcatcggc gagatcggca ccacgtggcc
     2221 ctggaccgac aacgagaaga aggtggtccg ggccgcggtg gccgcacagc gcgacactgg
     2281 cgcggccctc atgatccatc ccggccgcca cgaacgcctg ccgctggcaa tcgtggactt
     2341 catcagaaag gaaggcgccg atctcggccg cacgatcatg tgccacatcg agcgcacgat
     2401 cgccgatccc gccgtgctgc tcgagctggc cgcgaccggg gtgcgcctcg agtacgatct
     2461 cttcggcctg gagacctcgt actatcccta caagcccgcc ttcgacatgc ccaacgacgg
     2521 ggagcgcatg cgccagatcc tcttcctgat cgagcgcggc cacctcgacc agatcctgat
     2581 gtcgcacgac atcgcctaca agcactgcct gacccggtgg ggcggcttcg gctaccacca
     2641 cctgctcgtc aacgtgatcc cgcgcctgcg cgccaagggt gccgacgaca agatcgtcca
     2701 gacgctattg atcgacaacc ccaggcgggc cttcgtctac gcctgaagga cggaagggag
     2761 gaccaacgat gtcccctcgc ccgatcggtc cggttgtact cggcgcgctc ctgctcctgg
     2821 ccgctctgac gcccgcgtcc gcccaggcgc cggccggccc agccggagag atcgcggtct
     2881 ccttccacgt cacgctcgcg ccgagctggt tcgacccctc caccgccccg ccgcagatca
     2941 cgcccttcgg cgtgctgtac gcgatccacg acgcgctggt ccgcccgctg cccgggcaga
     3001 agatggcgcc gagcctggcc gagtcgtgga cggaaagcgc cgacgggctc gtctacgagt
     3061 tcaagctgcg ccgcgggctc aagttccaca acggcgaccc ggtcaccacc gacgacgtga
     3121 agtggagctt cgagcgctac cagggggcgg gcgccaagga gttccgggcg cgcatgcggc
     3181 aggtggagat cgtggatccg ctcgtgatcc ggtttcacct gaaggagccc tggccggact
     3241 tcatgacgtt ctacggcacc acggccaccg ccgccgccat cgtgctgccc cgcaaataca
     3301 cggagcaggc cgggcccgac ggcttccgcc agcggccgat cggagccggg ccctaccgct
     3361 tcgtgagcca gcgccagggc gtggacgtca cgctggaggc cttctcgggt cactggcgga
     3421 agacgccctt catcaagaag ctcaccatga agagcgtgcc cgacagcacc actcgactca
     3481 ccatgctgaa gagcggcgag accgacttcg ccctgttcct ggacggcccc gagggggagg
     3541 cggtgacgaa ggatccacgc atgcagctcg tggccacccg ccacgcgtcc atcttctggg
     3601 tcgagttcgc ggaccagtgg gatccgaagt cgccctggag cgaccggcgg ctgcgcctgg
     3661 ccgtcaacca cgcgctcgat cgcaagggca cgaacgaggc ggcctgcctt ggccactgcc
     3721 cgcccgccgg agtcatcgtg ccgcgcgtga tggacttcgc cctgcaggtc ccggcacccg
     3781 cctatgatcc gcagaaggcc cgccagctcc tcgccgaggc cggcttcccg aagggtctcg
     3841 acgcgggcga gttcgcggcc atcccgggct tcgccacggt aggcgaggca gcgctcaaca
     3901 acctgaacgc ggtcggcatc cggatgcgca tgcgtcccat ggagcgcgcc gccttctacg
     3961 cggcctggca ggagaagaag ctccgcgggc tcttcctcac cgcggtgggc aactccggca
     4021 acgcggccag ccgcgtggag tcgttcatgt actccaaggg caacgcggcc tacggcggtt
     4081 acccggacat cgacgagctg ttcctgcagc aggcccgcga gcgggatccc gccaagcgcg
     4141 agattctgct tcaccgcatc cagcagctca ccatcgaccg ggtcatgttc gcccccgtca
     4201 tggacctgcg cgggttgatc ggggtggggc cgcgggtggc ggagcacacc atcaactccg
     4261 tcccgatgta cccgttcccg tcctatgagg acgtgcggct gaagaacccg tagccggggc
     4321 ccggttgccg ctccccggcg cggagggcta tcatccgggg cgtggcagaa ggccaccggg
     4381 ccgtctcggt tctctaccag gcgctgctga ccggcctcgt cctcacgctg gtcacgcgcc
     4441 ggaccgagga cgaggccgcc gacttcgtct tccgcctgtt ccgccgccag cacctcgaga
     4501 agttcctgcc cgggctcgcc aagctcgggc tggacggcct gccgcccgcg gtggcctgcg
     4561 cgcaatacca ctacctctcc aaccagatgg gcggcgtgaa gaccgaatac gcctacgaat
     4621 ccgaccggaa ggcgtgggtg cgctacccgc cgccgcggtg gatctggtac ggctcggcga
     4681 tctgcggcat cccgagccgg gtgaacgcgg ccatcctgca cggctggcac ggccacaacg
     4741 gcgtgaccct cggcaatccg cgcctgggct tcgtctgcac cggccagacc gtggacggcc
     4801 acccgggcct cgaggggtac tacgtcgagc acgaccgtga cctcgcgccg gatgagcgcg
     4861 tgcgcttcgc cccgggcgag atcatgccgg atgccgatcc ggcgcgtgcc ccgcgcctcg
     4921 atccggcggc ctggccggcc gagcggctcg agctcgtggt ctcgcgctac gcgatggagt
     4981 acgtgcgcac ggcgctgccc gtcctgctcc agctgctggg gccccccgac acacggcggg
     5041 ttctgggcca cgccgcgcgg ctgatcggca tgcagtacca cgacgagacc gcccggctcc
     5101 tgggcatcgc ggactcgggc ccggctgcgt tcgcccgcta tctcgcggcg ctggccgccg
     5161 cccagggcga ggaggtggag gtctcgaccg agcccggccg agcccgcgtg cgccagaagg
     5221 gctggcgcct ggagcgcggc ctcgagccgc tcgatccggg cgcgttccac gcctgggccg
     5281 agctctggca cggcgcactc gccgcccacg accggcggct ccgcctgacg gtgacgcgcg
     5341 aaaccgacgc catcgacttc gacatcatct cacggtaagc ctggtcaccc tgctctgctc
     5401 ggtccgcggc tgtcgcctcc cgctcgctcg ggaggggcaa cgcctgacgt gcccacgcgc
     5461 gcactccttc gacgtggccc gccgcggcta cgtcaacctg ctgcagcctc aggaccgccg
     5521 ctcggcgcgg ccgggcgatt ccgccgccgc cgtcgcggcg cgccggcgtt tcctctcccg
     5581 cggcttcgag gcgacgctca cccgcgcgat cggggatctg ctgaccctca ccccggcgga
     5641 tgcgctcctc gaggtcggct gcggcgaggg ccactacctg ggcgccttca cggcgcggtt
     5701 cggctgcgag ggccacggcg tggacatctc ggtccccgcg atcgaggcgg cggccgcacg
     5761 gcacgcgggc ctgcacctcg tcgtggccaa cgccgaccgc gccctgccgt atgccgacgc
     5821 atcgttcgcg gtggtggcgt ccatcaccgc ccggcggaac ccggccgagt tccgccgcgt
     5881 cctccgcgac gacggccgcc tgctgctggt gatcccgggc ccggacgatc tcgtcgagct
     5941 ccgggaggcg gtcctcggcc gtggcctcct gcgggatcgg tcagatcagg cggtggccgc
     6001 gttcgcgccg cgattcgtac tcgagcgcca cgagcggatc cggcacatgg cgcgcctcga
     6061 cgccgcggcg atccacgacg tgatgaccgg ctcctaccgg gccgggcggg cgagccggcg
     6121 cgaccgtctg gccgcgctcg gcccgctcac cgtcaccctg agccgggacg cgctggtctt
     6181 gcgaccgatc agaccttgat caggccttga gctgctcccg gatgggcagg cgccggatgc
     6241 gcttgccggt ggccgcgaag atcgcgttgc agagcgccgg tgcgatgggc ggcacgccgg
     6301 gctcgccgac cccgcccatc ggctgaccgt agtcgccggg gatgatgtgc acgcgcgtct
     6361 cccgcggcgc ccccgacatg cgcgtgacct gataggtgtg gaagttgtcc tgctgcgccc
     6421 ggcccccctt gaaggccacc tcgccgagcg tcgcgatgcc cagccccatc accgcggcgc
     6481 cctgcatctg cgcgcgcacc cgatccgggt tgaccgtcgc gccgcagtcc accgcgacat
     6541 cgatgcgggg cacggtgatg acccccgcgt cgtcgaccgc cacctcgacc accgccgcca
     6601 cgtagctcac gaagctgtag tgcgcggcga tgcccaggcc ccggcccttg gggagctgcc
     6661 ggccccagcc cgcctcccgc gcggccgtct ccaccacgcg ccgcaggggt cgcggccggc
     6721 cgccgcggcc agctcggcca cgaaggattg caccgcgaag gcgtgcggga tgttggaaac
     6781 cgcgcggaac cagccgatgc gcgtgtgtgc ggcggcctcc gggttctcga tccggatgtt
     6841 ggcgatgtcg aacggcacgt tgatgacacc catgccgagc tcgagcgcgt gctcgttctt
     6901 ggcgccctcc acgaaggtgg agaagagcgt cggcgccacg ctccggtgca tccaggccac
     6961 cggcctgccc tgcgcgtcca cgcccgcctc cagatgctcc accgacaccg tgtggaagta
     7021 ggtgtgcatc aggtcgtcct cgcgcgtcca cgtcagcttg accggcttgc cctccatcgc
     7081 cttggacacg accgccgcct cgatcgcgaa gtcgggcttg gacttccgcc cgaacccgcc
     7141 gcccagtagc gtgacgtgca cggtcacgtc ggcgggctgg aggcccagcc gcttcgccac
     7201 gagatccttc gtggcctggg gcgactggaa gcacccccac acctcggctc ggctcccggt
     7261 gatgcggacg gtcgcggcgg gcggctccat cggcgcgtgc gcgaggtgcg ggatgtagta
     7321 ctccgcctcg atcttcttgg tggcggatgc catggccccg tccacgttgc cgttgtcgcg
     7381 gacgaccttg ccgggcttgc gcgccgactc ctccatcgcc gccttgaagg ccaccgagtc
     7441 gtaggtgccg ttgggcccgt cgttccagac caccttcagc gcccgccggc cctggatggc
     7501 cgcccaggta ttggtcgcga ccaccgccac gccgccgacc gggttgaact gggcgggcgc
     7561 cgggctcgca tcgatctcca ccaccttcag caccccggcc accttgaggc ctcggccgcg
     7621 tccacgctga cgaccttgcc cccgtagacg ggcggccggg ccacgaccgc gtagagcatg
     7681 ccgggcagcc gcgtgtcgat gccgtactgc gccctgccgt tcaccatggc cggggcgtcc
     7741 accagcttga tctggccccg gccgatgtag cggaacttcg agggatcctt gaggcgcacg
     7801 gtatcccgtg ccgggaccgg ctgcttggcc gcctccatgg cgagcgcgcc gtatccgaca
     7861 cgccgcccgc tcttggcgtg tacgacctcg tggttccggg cctccacctc ggtcaccggc
     7921 acgccccagc gcttggccgc cgcggcctcg agcatcgtgc gagccgccgc cccgcaacga
     7981 cgcatcggag tgaagaaatg ccgcgtgctg cgcgagccgt cggtgtcctg gttgccgaag
     8041 cgcttctcgt cgcccggggc ctgcaccacg cgcacccgct tccagtccgc ctcgag
//
LOCUS       DASUFA010000141         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_247762, whole genome shotgun sequence.
ACCESSION   DASUFA010000141 DASUFA010000000
VERSION     DASUFA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247762"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFX87_05070"
     CDS             complement(<1..1169)
                     /locus_tag="VFX87_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994314.1"
                     /translation="MAELILRNVRPMGGPAADVLVRDGMIARVGSGIEPAPGAPVIDG
                     DNGILLPGLVDAHMHLDKTFWGLPWRAHEAGPTTLERIENERRLRRELRLPADVQAER
                     LARQAVSRGTVHIRTHVDVDTEIGLRNFEGVMTARSRLREAVSIQVVAFPQSGVVTRP
                     GTAELLERAVKDGAELIGGVDPAGIDQDPKGQLDVVFGIAGRYGAGVDIHLHDPGDPG
                     AAQIRMIAERTRALGLGGKVAVSHAFCLGMVDEPEVARLVDLLREERIAIMTTAPGDR
                     PTPPVRRLREAGVAVGAGSDGVRDAWTPFGNADMLERAMLIAYRNGFRTDALVHGALD
                     VVTRGNAAVLGLARYGLAVGDRADFVVLAGETLGELVAMRPPRAWVISAGRVIAQ"
     gene            1260..2009
                     /locus_tag="VFX87_05075"
     CDS             1260..2009
                     /locus_tag="VFX87_05075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994315.1"
                     /translation="MDLKDRVALVAGGTGTLGIAIARALAKEGAHVAITYLERKDAAR
                     ETCAAVEALGRKAWSVALDQTDAGAIPPAIEAVVRQFGRLDLLVNNAAWNISIPFPDL
                     DALDAATWDRLFSTNLRGPYLLCRAAAPHLQASGSGRIVNIASVAGLNPGGSSIAYAT
                     SKAGLIHLTRCLAVALAPAVTVNCVAPGLMEGTRMTSRLRPELADGARQRAVLKRAAR
                     VEDVADQVAAFCRSDSVTGQVLNIDGGVFFH"
     gene            complement(2037..3716)
                     /locus_tag="VFX87_05080"
     CDS             complement(2037..3716)
                     /locus_tag="VFX87_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX4994316.1"
                     /translation="MTEPAKPSHFIRDAVLEDLQAGRFPRPVVTRFPPEPNGYLHIGH
                     AKAICLDFGLAQELGGRCHLRFDDTNPTKESQEYVDAITRDVHWLGFDWGTHLYFASD
                     YFDRLYEWARELIRAGKAYVDDLSADEIREHRGTLTEPGRESRWRNRPVEENLDLFQR
                     MRAGEFEDGSKVLRAKIDMAHPNLNLRDPVMYRIRKTAHHRTGDVWCVYPMYDWAHGQ
                     SDSIEGVTHSLCTLEYEDHRPLYDWYLDALGAYHPRQIEFARLSLSHTVMSKRKLLTL
                     VQDGLVSGWDDPRMPTLAGLRRRGYTPESLRDFSERIGVTKAANLVEVALLEHCLRED
                     LNRRALRRMAVLRPLRLVIENYPEGRTEEMEATNNPEDPGAGTRKVPFSRELWIERDD
                     FRETPPPKYFRLSPGVEVRLRAAYVIKCTGLTKDPATGEVTEVRATYDADTRGGEAKG
                     RKIKSTIHWVSAPHAVDAEVRLYDTLFTVPKPDEASDFKSVINPQSLEIVRGAKVEPA
                     VAQAAPEDHVQFERVGYFVADWKDHGPDRPVFNRSVGLRDTWARLEKTAGT"
     gene            3850..>4383
                     /locus_tag="VFX87_05085"
     CDS             3850..>4383
                     /locus_tag="VFX87_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX4994317.1"
                     /translation="MSEAVRVRFPPSPTGHLHMGGARTALFNWLYARHHRGVYVLRIE
                     DTDRSRSTEEYTQSLLEALRWLGLDWDEGPPTPGYRQTERFEIYRAHAERLLAEGRAY
                     RCRCTPETLDALRKAAEARKETFRYPGTCREARVPASEPHALRLAIPREGETVVDDVN
                     RGRVVFSHAELDDWILVR"
BASE COUNT          577 a         1588 c         1565 g          653 t
ORIGIN      
        1 ttctgggcga tgacccggcc ggcgctgatc acccaggcgc ggggcgggcg catcgcgacc
       61 agctcgccga gcgtctcgcc cgcgagcacc acgaagtcgg cgcggtcgcc gaccgcgagg
      121 ccgtagcggg cgaggccgag caccgccgcg ttgccccgcg tgacgacgtc gagcgccccg
      181 tgcaccagcg catcggtgcg gaagccgttc cggtaggcga tcagcatcgc gcgctcgagc
      241 atgtccgcgt tgccgaaggg cgtccaggcg tcccgcacgc cgtccgagcc cgcgcccacc
      301 gcgacgccgg cctcgcggag gcgccgcacc ggtggcgtcg gccggtcgcc gggcgcggtg
      361 gtcatgatcg cgatgcgctc ctcgcggagc aggtcgacga ggcgcgcgac ctcgggctcg
      421 tccaccatgc cgaggcagaa ggcgtggctg actgcgacct tgccgccgag cccgagcgcc
      481 cgggtccgct cggcgatcat gcggatctgc gcggcgcccg ggtcgccggg gtcgtgcagg
      541 tggatgtcca cgcccgcccc gtagcgcccc gcgatcccga agaccacgtc gagctgaccc
      601 ttcgggtcct gatcgatccc ggcgggatcc acgcccccga tcagctcggc gccgtccttc
      661 accgcgcgct cgagcagctc ggcggtgccc gggcgggtga ccacgccgct ctgggggaac
      721 gccacgacct ggatcgagac cgcctcgcgg aggcggctcc gcgcggtcat cacgccctcg
      781 aagttgcgca ggccgatctc ggtgtccacg tccacgtggg tccggatgtg cacggtgccg
      841 cggctcaccg cctgccgcgc cagccgctcc gcctgcacgt cggccggcag ccggagctcc
      901 cgccgcagcc gccgctcgtt ctcgatgcgc tcgagggtcg tcggcccggc ctcgtgcgcc
      961 cgccacggca gcccccagaa ggtcttgtcg aggtgcatgt gggcgtccac gaggcccggc
     1021 aacaggatgc cgttgtcccc gtcgatcacg ggggcgcccg gcgccggctc gatgccggag
     1081 ccgacccgcg cgatcatccc gtcgcgcacc agcacgtcgg cggcggggcc gcccatcggc
     1141 cgcacgttgc ggaggatcag ctcggccatg accgggcgag tgtacggccg tgccccggcc
     1201 gcttcaagcc cgggacgccc accgctccac gcctgacaat tctggtaggc taaggcgcca
     1261 tggacctcaa agatcgcgtc gccctggtgg cgggcggcac cggaacgctc ggcatcgcca
     1321 tcgcgcgagc cctcgcaaag gagggcgctc acgtcgccat cacctacctc gagcgcaagg
     1381 acgccgcccg ggagacctgc gccgcggtcg aagcgctcgg gcggaaggcg tggagcgtgg
     1441 ccctggatca gaccgacgcc ggcgcgatcc cgcccgccat cgaggcggtg gtgcgccagt
     1501 tcggccggct cgacctgctg gtcaacaacg cggcctggaa catcagcatc ccgttccccg
     1561 acctggacgc gctcgacgcc gcgacctggg accggctctt ctccaccaat ctccgcgggc
     1621 cgtacctgct ctgccgggcg gcggccccgc acctccaggc gagcgggtcc ggccgcatcg
     1681 tgaacatcgc gtcggtggcc gggctcaacc cgggcggcag tagcatcgcc tacgccacga
     1741 gcaaggccgg gctcatccac ctcacgcggt gcctcgcggt ggcgctggct cctgccgtca
     1801 ccgtgaactg cgtggccccg gggctgatgg aggggacgcg gatgacctca cggctgcggc
     1861 ccgagcttgc ggacggggcg cgccagcgcg ccgtgctcaa gcgcgcggcc cgcgtggagg
     1921 acgtggcgga tcaggtggcc gcgttctgcc gctcggacag cgtcaccggc caggtgctga
     1981 acatcgacgg gggcgtcttc ttccactgac cgacccggcg ggcgccgggt caggtctcaa
     2041 gtcccggccg tcttctcgag cctggcccac gtgtcgcgca ggcccacact gcgattgaag
     2101 acgggccggt cggggccgtg gtccttccag tccgcgacga agtagcccac ccgctcgaac
     2161 tgcacgtggt cctcgggcgc ggcctgcgcg acggcgggct ccaccttggc gcctcgcacg
     2221 atctcgagtg attgcgggtt gatcacgctc ttgaagtcgg acgcctcgtc gggcttcggc
     2281 accgtgaaga gcgtgtcgta gagccgcacc tccgcatcca ccgcgtgcgg cgccgagacc
     2341 cagtggatcg tggacttgat cttgcgcccc ttggcttccc cgccgcgggt gtcggcgtcg
     2401 tacgtggcgc gcacttcggt cacctcgccg gtggccgggt ccttggtgag cccggtgcac
     2461 ttgatcacgt aggccgcgcg cagccgcacc tcgacgccgg gtgagaggcg gaagtacttg
     2521 ggcggcggtg tctcgcggaa gtcgtcccgc tcgatccaga gctcgcgcga gaaggggacc
     2581 ttgcgggtac ccgcgcccgg gtcctcggga ttgttggtcg cctccatctc ctcggtccgg
     2641 ccctcggggt agttctcgat gaccaggcgc agcggccgca gcaccgccat gcgccggagc
     2701 gcgcgccggt tcaggtcctc gcgcaggcag tgctccagca gggcgacctc gaccaggttc
     2761 gcggccttgg tcacgccgat gcgctcgctg aagtcgcgca gggactcggg cgtgtagccg
     2821 cgccgccgca ggccggccag ggtcggcatc cgcgggtcgt cccacccgct caccaggccg
     2881 tcctgcacca gcgtgagcag cttgcgcttg gacatcacgg tgtggctcag gctcaggcgt
     2941 gcgaactcga tctgccgggg gtggtaggcg ccgagcgcgt cgaggtacca gtcgtagagc
     3001 ggccggtggt cctcgtactc cagcgtgcac agcgagtggg tgacgccctc gatcgagtcg
     3061 gactggccgt gtgcccagtc gtacatcggg tacacgcacc agacatcgcc ggtgcggtgg
     3121 tgggcggtct tgcggatgcg gtacatgacc gggtcgcgca ggttgaggtt gggatgggcc
     3181 atgtcgatct tggcgcgcag gaccttgctc ccgtcctcga actcgcccgc gcgcatgcgc
     3241 tggaagagat ccaggttttc ctccaccggc cggttccgcc accggctctc ccgtccgggc
     3301 tcggtcagcg tgccgcggtg ctcgcggatc tcgtccgccg agaggtcgtc cacgtaggct
     3361 ttcccggcgc ggatgagctc ccgcgcccac tcgtagagcc ggtcgaagta gtcactcgcg
     3421 aaatagaggt gggtacccca gtcgaagccg agccagtgga cgtcgcgcgt gatcgcgtcc
     3481 acgtactcct ggctctcctt ggtggggttg gtgtcgtcga agcgcaggtg gcagcgcccg
     3541 cccagctcct gggccaggcc gaagtcgaga cagatcgcct tggcgtggcc gatgtgcagg
     3601 tagccgttgg gctccggcgg aaaccgggtg accaccggac gggggaagcg gccggcctgg
     3661 agatcttcca ggacggcgtc ccggatgaag tgggagggct tggccggctc ggtcatggac
     3721 ctgatcctaa catcggttgt ctcccgggcc gccgatcggg ggcgtctcgg gcttcgcggc
     3781 cgccgcggcc cgcgggtggc atctgacgcg ggacggccgc tcggcaagtg ctgatacaat
     3841 aaggctccta tgtccgaggc ggtgcgcgtt cgctttcccc caagtccgac tgggcatctc
     3901 catatgggcg gcgcccgcac cgcgctcttc aactggctct acgcgcgcca ccaccggggc
     3961 gtgtacgtgc tccggatcga ggacaccgac cgctcgcgct ccaccgagga gtacacccag
     4021 tccctcctcg aggccctgcg ctggctcggg ctcgactggg acgagggccc gcccacgccc
     4081 ggctatcgcc agaccgagcg cttcgagatc tatcgcgcgc acgccgagcg gctgctcgcg
     4141 gagggccgag cctaccgctg ccggtgcacg ccggagacgc tggacgcgct gcggaaggcc
     4201 gcggaggcgc gcaaggagac cttccgctac ccgggcacct gccgcgaggc ccgcgtgccc
     4261 gcctcggagc cccacgccct gcgcctggcg atcccgcgcg agggtgagac ggtggtggac
     4321 gacgtgaacc gcggccgtgt ggtgttcagc cacgccgagc tcgacgactg gatcctcgtc
     4381 cgg
//
LOCUS       DASUFA010000142         4139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1522603, whole genome shotgun sequence.
ACCESSION   DASUFA010000142 DASUFA010000000
VERSION     DASUFA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522603"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1612
                     /locus_tag="VFX87_05090"
     CDS             14..1612
                     /locus_tag="VFX87_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4994318.1"
                     /translation="MKPGAPIPATLTELMGGRAAQDPDTPYFHLFDEAVPYGRLWRES
                     ARYAAGLRRAGIDRGDKVCLIYPTCAEFFYTFFGALRLGAVPVPLYPTLGVEATANIF
                     RDSDAKAVVTIGWFRQGVEESQAAASNIRHVLEPSDLEADDPAPPFPTAAADDVAFIQ
                     YTSGSTGQPRGVVLSHANVVRTVEFMAEAAQLTRDDVVVSWLPLYHDMGLIGCSFTPP
                     WSGAPLHLLPPDLKNPRVWLELVTRVNATFTVSPDFGYRNCVRNIHDTSGLDLSSLKQ
                     ALSGAEPVRLSTIEAFERKFDAQNLITPCYGLAEATLAVAIWPRRTPLRLDSSGRFLS
                     VGQPCRGVRVRIMDGEEEVKPGVEAEICVSSPGVMQGYYNNPEATRKILSPDGWLRTG
                     DLGFVDAEGFLYVTGRLKDVIILGGENVIPADIEEIVDHVPGVRYSAAVGVESERTGT
                     QRLHVVAEIREQAAEVADHSRVVREIVQRVREGRGHRPARVLLVLPSTIPKTSSGKIQ
                     RSRLGEMIARDELRDRIVYSSGAH"
     gene            1633..2526
                     /locus_tag="VFX87_05095"
     CDS             1633..2526
                     /locus_tag="VFX87_05095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4994319.1"
                     /translation="MYGPVGTRHGVITFARRMEALGYDSLWASDHVVIPHRMTTPYPY
                     SPTGKFPLTPEVPFLEPLTTLALVAGVTEHVRLGTSILVLPHRNPVLAAKMAATLDHL
                     SGGRLVLGVGVGWMREEIELLGGNYDRRGAWSDEALTVMRACWRDDRTEHHGEFFSFD
                     GIGVFPKPTRGDIPILIGGHTPLALRRVVELGDGWHAAFITPDALKADIARLREECAR
                     QHRPFDQISISVRAGLSLRDPPLGADRKPLQGSREQVIGDLAAFRALGVESMLLETRY
                     RDLDDMIAIYEAFAADIRPNI"
     gene            complement(2566..3300)
                     /locus_tag="VFX87_05100"
     CDS             complement(2566..3300)
                     /locus_tag="VFX87_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX4994320.1"
                     /translation="MPARSLKPAASIAARLERVDWAEVARSLDERGWATTPALVTAAE
                     CAELIRLYADERRFRSRVDMARFRFGEGEYKYFANPLPLVVRELRRHAYPHLAPMASG
                     WEATLGTDRSYPPDLDGFLAVCAEHGQTKPTPLLLRYEAGGYNCLHQDLYGDVAFPLQ
                     MTCLLSRPGVDFTGGEFLLVEQRPRAQSTGSVVPLERGQIVIFATRHRPTRGSRGAYR
                     VTLRHGVSRVVSGERYTLGVIFHDAA"
     gene            complement(3282..4007)
                     /locus_tag="VFX87_05105"
     CDS             complement(3282..4007)
                     /locus_tag="VFX87_05105"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4994321.1"
                     /translation="MNDGKEGRDQLDRLLADYFRPETSPADMGRRLAAAARASETALT
                     RLEGKLGIEATARGISLVRTGPAGKPATAAARKLTEQAREEIREYLRGQRAFFRVPVD
                     LALVPDFQRKVLEAARLIPFGEARPYAWIAARIGNPRAVRAVGTALGRNPVPLILPCH
                     RVWRSDGGFGGYIFGAAVKDALAALERTTPVLEGCTSTRIVCRVGCIHGRHMRPDNRV
                     VFASVDDARSVGYRPCKVCRPAA"
BASE COUNT          621 a         1560 c         1345 g          613 t
ORIGIN      
        1 tagaatcacg cccgtgaagc caggagcacc catcccggcc accctgaccg agctgatggg
       61 gggcagggcc gcccaggatc ctgacacccc gtacttccac ctcttcgacg aagcggtgcc
      121 gtacggccgt ctgtggcggg agagcgcgcg ctacgcggcc gggctcaggc gcgccggcat
      181 cgaccgcggc gacaaggtct gcctgatcta cccgacctgc gccgagttct tctacacctt
      241 cttcggcgcg ctcaggctgg gggcggttcc ggtcccgctc taccccaccc tcggggtgga
      301 ggcgaccgcc aacatcttcc gcgactcgga cgccaaggcg gtggtcacca tcggctggtt
      361 ccgccagggc gtggaggaat cgcaggccgc cgcgtccaac atccggcacg tgctcgagcc
      421 gagtgatctc gaggcggacg atcccgcgcc gcccttcccg accgcggccg ccgacgacgt
      481 ggccttcatc cagtacacct cgggcagcac cggccagccg cgcggggtcg tgctgagcca
      541 cgccaacgtg gtgcgcaccg tcgagttcat ggcggaggct gcgcagctca cccgggacga
      601 cgtggtcgtc tcgtggctgc cgctctacca cgacatgggc ctgatcggct gctcgttcac
      661 cccgccctgg agcggggcgc cgctgcacct gctgccgccc gatctcaaga acccgcgggt
      721 gtggctcgag ctggtcactc gcgtcaacgc caccttcacg gtctcgcccg acttcggcta
      781 ccgcaactgc gtgcgcaaca tccacgacac gagcggcctc gatctctcct cgctcaagca
      841 ggcgctctcc ggcgccgagc cggtgcgctt gagcaccatc gaggccttcg agcggaaatt
      901 cgacgcgcag aacctcatca cgccctgcta cggcctggcg gaggccaccc tcgcggtggc
      961 gatctggccg cggcgcaccc cgctccgcct ggacagctcg ggccggttcc tttcagtggg
     1021 ccagccctgc cgcggcgtgc gcgtgcgcat catggacgga gaggaagagg tcaagcccgg
     1081 cgtcgaggcc gagatctgcg tatcgagccc cggggtgatg cagggctact acaacaatcc
     1141 cgaggccacc cgcaagatcc tctctccgga cggctggctc cgcaccggcg acctcgggtt
     1201 cgtggacgcc gagggcttcc tctacgtcac cggccggctc aaggacgtca tcatcctggg
     1261 cggcgagaat gtgatccccg ccgacatcga ggagatcgtg gaccacgtgc cgggcgtgcg
     1321 ctactccgcc gcggtcggcg tcgagagcga gcgcaccggc acccagcgcc tgcacgtagt
     1381 ggccgagatc cgcgagcagg ccgccgaggt ggccgatcac tcgcgggtgg tccgcgagat
     1441 cgtccagcgc gtccgggagg gacggggcca ccgccccgcc cgcgtgctcc tggtcctgcc
     1501 cagcaccatc cccaagacct cgtcgggcaa gattcagcgc tcgcgcctcg gcgagatgat
     1561 cgcgcgtgac gagctgcgtg atcgcatcgt ctactcctcc ggcgcccatt gaggatcggc
     1621 tgtcacctgc ccatgtacgg tcccgtgggc acgcgccacg gggtgatcac cttcgcgcgt
     1681 cgcatggagg ccctcggcta cgactcactc tgggcgagcg atcacgtggt gatcccgcac
     1741 cggatgacga cgccctatcc gtacagcccc accgggaagt tcccgctcac ccccgaggtg
     1801 ccgttcctcg aaccactcac cacgctggcc ctggtggcgg gcgtcaccga gcacgtgcgg
     1861 ctcggcacca gcatcctggt gctcccgcac cggaacccgg tcctcgccgc gaagatggcc
     1921 gccaccctcg accatctctc cgggggccgg ctggtgctcg gggtgggcgt gggctggatg
     1981 cgcgaggaga tcgagctgct gggcggcaac tacgaccgac gcggcgcctg gagcgacgag
     2041 gcgctcaccg tcatgcgcgc ctgctggcgc gacgaccgta ccgagcatca cggggagttc
     2101 ttctccttcg acgggatcgg tgtcttcccc aagcccaccc gtggcgacat cccgatcctg
     2161 atcggcgggc acacgccgct tgccctccgc cgcgtcgtcg agcttggcga cggctggcac
     2221 gccgccttca tcacgccgga cgcgctcaag gccgacatcg cgcggttgag agaggagtgc
     2281 gcccgccagc atcgcccctt cgatcagatc tcgatcagcg tgcgcgccgg cctgtctcta
     2341 cgcgaccctc cgctcggcgc cgaccgcaag cccttgcagg gctcgcgcga gcaggtgatc
     2401 ggcgacctcg ccgccttccg tgccctcggc gtcgagtcca tgctcctcga gacccgctac
     2461 cgcgacctcg acgacatgat cgccatctac gaggcgttcg cggccgacat ccgcccgaac
     2521 atctgagccc cctctcccct ctggggaaag ggcagggtga cgggcctacg ccgcgtcgtg
     2581 aaagatcaca cccaacgtgt aacgctcgcc tgaaaccaca cggctgacgc cgtggcgcag
     2641 cgtcacgcga taggcgcccc gtgagcctct tgtcggccga tgccgcgtgg caaagatcac
     2701 gatctgcccg cgctccaagg gaacgaccga cccggtcgac tgcgctcgcg gccgctgctc
     2761 gaccagcagg aactcgcctc cggtgaagtc gacgcccgga cgactcagca agcaagtcat
     2821 ctggagcgga aacgccacgt caccatacag atcctggtgc aagcagttgt acccgccggc
     2881 ctcgtaccgc aacagcagcg gcgtgggttt cgtctggccg tgctcggcgc aaaccgcgag
     2941 gaagccgtcg agatcgggcg ggtagctccg atcggtcccg agcgtggcct cccagccgct
     3001 cgccatcggc gcgagatgcg gatacgcgtg acgccgcagc tcgcgcacga caagcggcag
     3061 cggattcgcg aagtacttgt actccccttc cccgaagcga aaccgcgcca tgtccacgcg
     3121 actgcggaac cgccgctcgt ccgcgtagag ccggatcagc tccgcgcact ccgccgcggt
     3181 gaccagcgcg ggcgtggtgg cccagccccg ctcatcgagg gagcgggcca cctccgccca
     3241 gtccacccgc tcgagccgcg ccgcgatgga agcggctggc ttcaggctgc gggccggcat
     3301 actttgcacg gccggtaccc caccgagcgc gcatcgtcca ccgaggcgaa caccacccga
     3361 ttgtccgggc gcatgtgccg gccgtggata caccccacgc ggcacacgat gcgcgtgctc
     3421 gtgcagccct ccagcacggg cgtggtccgc tcgagggcgg ccagcgcgtc cttcaccgcc
     3481 gcgccgaaga tgtagccgcc gaagccgccg tcgctccgcc acacgcggtg gcagggtagg
     3541 atgagcggca ccgggttgcg gccgagcgcg gttccgaccg cccggaccgc gcggggattg
     3601 ccgatgcgcg ccgcgatcca cgcgtacggg cgcgcctcgc cgaacgggat gagccgcgcg
     3661 gcctccagga cctttcgctg aaagtccggc accaaggcga gatccaccgg cacccggaag
     3721 aacgcccgtt ggccccgcag gtactcgcgg atctcctccc gcgcctgctc ggtcagcttc
     3781 cgcgccgccg cggtggccgg cttcccggcc gggccggtgc ggacgagcga gatcccgcgc
     3841 gcggtcgcct cgatgccaag cttcccctcg agccgcgtca gcgcggtctc ggacgcccgg
     3901 gccgccgccg cgagccggcg tcccatgtcc gcgggcgacg tctccggccg gaagtagtcc
     3961 gccagcagcc ggtcgagctg gtcccgcccc tccttgccgt cattcatggc ctgctcctcg
     4021 cttgccgagc tcatctcgca gcagggcctt gccccgcgcg agctgactct tcacggtatt
     4081 gatcgtgatg cctcggatct ttgccagctc cccgtaggag agcccatcca cgtagcgga
//
LOCUS       DASUFA010000143         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_835639, whole genome shotgun sequence.
ACCESSION   DASUFA010000143 DASUFA010000000
VERSION     DASUFA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835639"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFX87_05110"
     CDS             <1..778
                     /locus_tag="VFX87_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994322.1"
                     /translation="RDGAVRESLTRAAGAGDGGLKPKRVTVEDGIASHKHPVVFFQRP
                     GADAVAGELEALIRGVSLASPAAPARCRLCGSDSGAEPVLVNDVVDRVCPGCAERLQH
                     EARQASARYDEAPMNLPLAVAAAAVLAVAGAAAWAGIAIATNRVFWLVAIGVGLAIGW
                     VTTKAAGKAGRVVQVVGALATVVSVLLGELFLVGYHVQQQARRGGQEVDWVSFFASAP
                     TILWQLGGETLFALGGGLIGAWYAARLAARPKIEVRVEKV"
     gene            810..1568
                     /locus_tag="VFX87_05115"
     CDS             810..1568
                     /locus_tag="VFX87_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX4994323.1"
                     /translation="MPTLTPAQLASYARDGYLVLPGLFDPAAAARLAAWTDEVTAWPE
                     TPGRHMVYWEAHRHEPGRRVRSRIENFYPYHTGFRATFDGGPLAAVAAALLDGPAVLF
                     KDKINFKMPGGEGFEPHQDQQAGWSAYAPLFLTALVSIDAATRENGCLELAAGHHRRG
                     LVGQEWKPLGEAEMAGMRFEACPTRPGDAVFFDSYVPHRSEPNATAAPRRVLYVTYNR
                     RADGDHRLRYYADKRRSFPPDCEREPGREYVYRV"
     gene            complement(1556..>2033)
                     /locus_tag="VFX87_05120"
     CDS             complement(1556..>2033)
                     /locus_tag="VFX87_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4994324.1"
                     /translation="PMRYFEDFKVGEVIAGGARTITRDEIVAFAREFDPQPFHTDEAA
                     ARETMYGGLIASGWHSGSLMMRLFYESLIGHSASMGSPGIDELRWLKPVRPGDTLRLR
                     TTVLEVIESRSKPDRGLVRTFCELLNQDGEVVMSIKPVNFFRRRPAGGAGAAGQTR"
BASE COUNT          267 a          727 c          756 g          283 t
ORIGIN      
        1 gcgcgacggg gcggttcgcg agtctctgac ccgcgccgcc ggggccggcg acggcgggct
       61 caagcccaag cgcgtgacgg tggaggacgg gatcgcctcc cacaagcacc ccgtggtgtt
      121 cttccagcgg ccgggggccg atgcggtggc gggcgagctg gaggccctca tccgcggggt
      181 gagcctggcg agccccgccg cccccgcgcg ctgccggctc tgcgggagcg atagcggcgc
      241 tgagcccgtg ctggtcaacg acgtggtgga ccgcgtctgc ccgggctgcg cggagcggct
      301 ccagcacgag gctcggcagg cgagcgcgcg ctacgacgag gcccccatga acctgcccct
      361 ggccgtcgcc gcggccgcgg tgctggcggt ggcgggcgcg gcggcctggg ccggcatcgc
      421 catcgccacc aaccgcgtgt tctggctcgt ggccatcggc gtcgggctcg ccatcggctg
      481 ggtcaccacc aaggcggcgg gcaaggccgg gcgggtggtg caggtggtgg gagcgctggc
      541 caccgtggtc tcggtgctgc tgggcgagct cttcctggtg gggtatcacg tgcagcagca
      601 ggcccggcgc ggcggccagg aggtggactg ggtctccttc ttcgcgtccg cccccaccat
      661 tctctggcag ctcggcggcg agacgctctt cgcgctgggc ggcggcctca tcggcgcgtg
      721 gtacgcggcg cggctggccg cccggccgaa gatcgaggtg cgcgtcgaga aggtgtgagc
      781 cgcggcgcct cacgtatact ggatctccca tgccgaccct gacgcccgcg cagctcgcct
      841 cctacgcccg cgacggctat ctcgtgctgc ccggcctctt cgatcccgcc gcggccgcgc
      901 gcctcgccgc atggacggac gaggtcaccg cctggccgga gacgcccggc cggcacatgg
      961 tgtactggga ggcgcaccgg cacgagccgg gccgtcgcgt gcggagccgc atcgagaact
     1021 tctatcccta tcacacgggc ttccgcgcca ctttcgacgg cgggccgctg gcggcggtgg
     1081 cggcggcgtt gctcgacggc cccgcggtcc tcttcaagga caagatcaac ttcaagatgc
     1141 cgggcggcga gggcttcgag ccccaccagg accagcaggc gggctggtcc gcctatgccc
     1201 cgctcttcct caccgcgctg gtcagcatcg acgccgccac gcgggagaac ggctgcctcg
     1261 agctggcggc cgggcatcac cggcgcgggc tggtgggaca ggagtggaag ccgctgggcg
     1321 aggcggagat ggcggggatg cgcttcgagg cctgccccac gcggccgggc gacgcggtgt
     1381 tcttcgactc ctacgtgccg caccgctcgg agccgaacgc gacggcggcg ccccggcgcg
     1441 tgctctacgt cacctacaac cggcgcgccg acggcgatca ccggctccgc tactacgccg
     1501 acaagcggcg cagcttcccg cccgactgcg agcgggagcc cggccgggag tacgtctacc
     1561 gcgtctgacc cgccgcgccg gcgccccccg ccggccgccg ccgaaagaag ttcaccggct
     1621 tgatcgacat caccacttcg ccgtcctgat tgagcagctc gcagaaggtg cggacgaggc
     1681 cgcgatcggg cttggaccgc gattcgatca cctccagcac ggtcgtgcgc aggcgcagcg
     1741 tgtcgccggg acggacgggc ttgagccagc gcagctcgtc gatgccgggg gagcccatgg
     1801 aggcgctgtg gccgatgagc gactcgtaga agaggcgcat catcagcgag cccgagtgcc
     1861 atccgctggc gatgaggccg ccgtacatgg tctcgcgcgc ggccgcctcg tcggtgtgga
     1921 agggctgcgg gtcgaactcg cgcgcgaagg ccacgatctc gtcccgggtg atggtgcgcg
     1981 caccgcccgc gatcacttcg ccgaccttga aatcctcgaa atagcgcatg gga
//
LOCUS       DASUFA010000144         5304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_34290, whole genome shotgun sequence.
ACCESSION   DASUFA010000144 DASUFA010000000
VERSION     DASUFA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34290"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VFX87_05125"
     CDS             <1..144
                     /locus_tag="VFX87_05125"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HEX4994325.1"
                     /translation="AEYGASGVMTFIVNHDGIVYQKDLGPSTAATARALTHFDPDPTW
                     SRF"
     gene            261..2045
                     /locus_tag="VFX87_05130"
     CDS             261..2045
                     /locus_tag="VFX87_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HEX4994326.1"
                     /translation="MAQLTWRAVLMGSLLGGMLSLTNLYIGLKAGWGFGVAITASILS
                     YAIWSVLYRLGLARTPMSILENNCMQSTASAAGYSTGGTLISAFAAYMLINDRALSLP
                     VMLAWIFFLSVLGVTMAIPMKRQMINIEQLRFPSGIAAAETLRALHSVGDRGARSARA
                     LGWAGLGAVLGKLWAEGLVLVSAKLAPFMIGTWIAAANERLVGPAWIGRTVMLSWEPM
                     FVAAGAITGWHVGWSMLLGSVSAWMIFVPVLQHQGVIEGSGYGTLVQWTLWGGVACMV
                     TSSLVSFAMQWRTARRAFADLGAMFAGGGRAADDPLAAIETPASWFLWGQVVGFAGLA
                     WLGHVTFGMPYWQTAAAVLLSFVLALVACRVTGETDTTPVGAMGKITQLFFGAVSPGN
                     MNVNLMSAAVTAAAASSSADLLTDLKSGYLLGAHPRRQFLAQFAGIFVGTLVSVLSFR
                     LLVPDASALGGDQFPAPAAQAWRAVALALSHGVLALGPVKTWSIAVGALVGIVLSVLP
                     AMFPARRHLFPSPAGLGLAWTFHWHYGLLFCLGGGLGWWAEKKYPRWAEELTFPVASG
                     WIAGESLMGVLLVMWQNGPELVRRLTGH"
     gene            complement(2077..4476)
                     /gene="ppsA"
                     /locus_tag="VFX87_05135"
     CDS             complement(2077..4476)
                     /gene="ppsA"
                     /locus_tag="VFX87_05135"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX4994327.1"
                     /translation="MTVFIKWLREIRLGDVAQVGGKTASLGELYGELGAAGVRVPDGF
                     AVTADAYRALLDRDGLRDRLDALLKGVTGEDVTALATAGAELRRLVESAPLPSGLEDA
                     IVSAYRTLAREAGADQPAVAVRSSATAEDLPQASFAGQHESFLGVRGEAALLTACRRC
                     FASIFTDRAIVYRIQNGFDHLTVFLSVAVQRMVACDRGSSGVMFTLDPDSGFPDVVLV
                     NAAFGLGEAVVQGQLDPDEFWIFKPTLRTGHHALLKRKIARKTWKLVMGAGGQPERAP
                     VPEEAQRSASLSDPEALELGRFAVAIEEHYSRRAGHVVPMDIEWARDADDGRLYILQA
                     RPETVHRAARRAVLEIFSLRPGGPRDRLVSGAAIGQRIGVGPTRRLCDPRDLEAFRAG
                     DVLVASMTDPDWEPIMKRAAAIVTDRGGRTCHAAIVSRELGVPCVVGTGNGTELLPDG
                     QLVTVSCAEGETGSVYAGALSVERRQVDLAAMPRTRTRVMLNVGNPGEAFRLAALPND
                     GVGLARIEFIIASFVRAHPLALLHPDRVRDPAARAEIDGLTAGYADRGEYFVERLAEG
                     VATIAAAFYPKDVIVRLSDFKSSEYAGLVGGRDFEPEEGNPMLGFRGAFRYAHPRYRD
                     AFGLECRALRRVREQMGLANVKLMIPFCRTPAEGRRVLELLAAGGLGRGVNGLEVYVM
                     AEIPANAILVDEFAALFDGFSIGSNDLTQLVLGVDRDSELVAPEFDERNPAVLSMIGM
                     IIDGARRAGRKVGICGQAPSDYPEMVRFLVGKGITSISLNPDAVVRGLEAVAAAEADR
                     R"
     gene            complement(4498..>5304)
                     /locus_tag="VFX87_05140"
     CDS             complement(4498..>5304)
                     /locus_tag="VFX87_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4994328.1"
                     /translation="LTPEDVDGASKVVVLGQSVAENLFGGTDPIDQTVRIKAVPFTVV
                     GVLARKGQSTRGEDQDDTLMIPLSTAKKKVLGVSQANARSVGSILVKVADPGRMAEAE
                     AQVRDLLRERHRLQPHEEDDFAIRNLSEILASQEAASRVLSLLLAAVAAVSLVVGGIG
                     IMNIMLVSVTERTREIGLRRAVGARRRDILSQFLIEAVFVSLAGGLIGIGVGIVGSYA
                     IGFVFQWNVLVSWSAVVPAFLFAAAVGVFFGYYPARKASRLKPIEALRYE"
BASE COUNT          744 a         1960 c         1820 g          780 t
ORIGIN      
        1 gccgagtacg gcgcctcggg cgtgatgacc ttcatcgtca accacgacgg gatcgtctac
       61 cagaaggacc tgggcccgag caccgcggcg accgcgcggg cgctcaccca cttcgacccc
      121 gatccgacct ggtcgcgctt ctgagccacc cgccccccgc ggccgagctc gacgccctcc
      181 ccgtccgcgg gttccagggt cgcgccgaag acatcgagcg gcagtggtac gagcaggtct
      241 accgcggccg cggcgaccgg atggcccagc tcacgtggcg cgcggtgctg atgggctcgc
      301 tgctgggcgg gatgctctcc ctcaccaacc tctacatcgg gctcaaggcg ggctggggct
      361 tcggggtcgc catcaccgcc tccatcctct cgtacgcgat ctggagcgtg ctctaccggc
      421 tgggcctcgc gcgcaccccg atgagcattc tcgagaacaa ctgcatgcag tccaccgcga
      481 gcgcggcggg ctactccacg ggcgggacgc tcatctccgc cttcgcggcc tacatgctca
      541 tcaacgaccg agcgctgtcc ctcccggtga tgctcgcgtg gatcttcttc ctgtcggtgc
      601 tcggggtgac catggccatc cccatgaagc gccagatgat caacatcgag cagctccgct
      661 tcccgagcgg catcgcggcg gccgagacgc tccgggcgct ccactcggtc ggcgaccgcg
      721 gcgcgcgctc ggcccgggcc ctcggctggg ccgggctggg cgcggtgctg gggaagctct
      781 gggcggaagg gctcgtgctg gtgagcgcca agctcgcccc cttcatgatc ggcacctgga
      841 tcgccgcggc caacgagcgc ctggtcggcc cggcctggat cgggcgaacc gtgatgctct
      901 cctgggagcc gatgttcgtc gcggcgggcg ccatcaccgg ctggcacgtg ggctggagca
      961 tgttgctcgg cagcgtcagc gcgtggatga tcttcgtccc cgtcctccag caccagggcg
     1021 tcatcgaggg ctcgggctac gggaccctcg tgcagtggac gctctggggc ggcgtggcgt
     1081 gcatggtcac ctcgagcctc gtctccttcg cgatgcagtg gcgcaccgcg cggcgcgcct
     1141 tcgccgatct cggcgcgatg ttcgccggcg gcggccgcgc cgcggacgac ccgctggccg
     1201 cgatcgagac gcccgcctcc tggttcctgt gggggcaggt cgtgggcttc gccggtctcg
     1261 cctggctcgg ccacgtcacc ttcggcatgc cctactggca gacggcggcg gcggtgctcc
     1321 tctcgttcgt gctggccctc gtcgcctgcc gcgtcaccgg ggagaccgac accaccccgg
     1381 tgggcgccat gggcaagatc acccagctct tcttcggcgc ggtgagcccg ggcaacatga
     1441 acgtgaacct catgagcgcc gcggtcaccg ccgcggccgc gagcagcagc gccgacctgc
     1501 tcaccgacct caagagcggc tatctcctcg gggcgcaccc tcgccggcag ttcctcgcgc
     1561 agttcgccgg catcttcgtc gggacgctcg tcagcgtgct gtcgttccgg ctcctcgtgc
     1621 cggacgcgag cgcgctgggt ggggaccagt tcccggcgcc cgcggcccag gcgtggcgcg
     1681 cggtggcgct cgcgctgtcc cacggcgtcc tcgcgctcgg gcccgtcaag acgtggtcca
     1741 tcgcggtcgg ggccctcgtc ggcatcgtcc tctccgtgct ccccgcgatg ttccccgcgc
     1801 gccggcacct cttcccgtca ccggccgggc tcggtctggc gtggaccttc cactggcact
     1861 acgggctgct cttctgcctg ggcggcggac tcggctggtg ggcggaaaaa aagtatcccc
     1921 gatgggcgga ggagctcacc ttcccggtgg cctcgggctg gatcgcgggc gagagcctga
     1981 tgggcgtgct gctcgtgatg tggcagaacg ggcccgagct cgtgcgccgg ctgaccggcc
     2041 actagcacgc gtcaggtgga gctcccgcga gaccgctcat cgccggtcgg cttccgccgc
     2101 cgcgacggct tcgaggccgc gcaccaccgc atccggattg agcgagatgc tggtgatgcc
     2161 cttccccacc aggaaccgga ccatctcggg atagtcgctc ggcgcctggc cgcagatgcc
     2221 gacctttcgt cccgcgcgcc gtgccccgtc gatgatcatg ccgatcatgc tgagcaccgc
     2281 cgggttgcgc tcgtcgaact cgggcgcgac gagctccgag tcccggtcca ccccgagcac
     2341 gagctgggtg agatcgttgg agccgatcga gaagccgtcg aagagcgccg cgaactcgtc
     2401 gaccaggatc gcgttggcgg gaatctcggc catcacgtag acctcgagac cgttcacgcc
     2461 gcgcccgagc ccgccggcag ccagcagctc gaggacgcgc cggccctcgg cgggagtccg
     2521 gcagaacggg atcatcagct tcacgttggc gaggcccatc tgctcgcgga cccggcgcag
     2581 ggcccggcac tcgagcccga acgcgtcccg gtagcgcggg tgcgcgtagc ggaatgcccc
     2641 ccggaaaccg agcatgggat tgccttcctc gggctcgaag tcccgaccgc ccacgagccc
     2701 cgcgtactcg ctggacttga agtccgagag gcggacgatc acgtccttcg ggtaaaaggc
     2761 cgccgcgatc gtcgccacgc cctcggcgag ccgctcgacg aagtattccc cgcgatccgc
     2821 atacccggcg gtcagcccgt cgatctccgc ccgcgcggcc ggatcgcgga cgcggtccgg
     2881 gtggagcagc gcgagcggat gcgcacgcac gaagctcgcg atgatgaact cgatgcgcgc
     2941 cagccccacg ccgtcgttgg gcagggcggc cagccggaat gcctcgccgg gattgccgac
     3001 attgagcatc accctggtcc gggtcctcgg catcgcggcc aggtccacct gccggcgctc
     3061 gacggacagc gcgcccgcgt agacggaccc ggtctcgcct tcggcgcagg acacggtcac
     3121 gagctgcccg tcggggagga gctccgtccc attgccggtg ccgaccacac acggcacgcc
     3181 gagctcgcgg ctgacgatgg cggcgtggca ggtgcggccc ccgcggtcgg tcacgatggc
     3241 cgccgcgcgc ttcatgatgg gctcccagtc gggatcggtc atggaggcga cgagcacgtc
     3301 cccggcccgg aaggcctcca ggtcgcgcgg atcgcacagc cgccgcgtcg gcccgacccc
     3361 gatccgctga ccgatggccg cgccgctgac gagccggtcc cgcggcccac ccggccgcag
     3421 cgagaagatc tcgaggacgg cccggcgcgc ggcgcggtgg accgtctcgg gccgcgcctg
     3481 gaggatgtag agccggccgt cgtcggcatc cctcgcccat tcgatgtcca tcggcaccac
     3541 atgccccgcc cgccgcgagt agtgctcctc gatggcgacc gcgaagcggc cgagctccag
     3601 cgcctccggg tcactgaggc tggcggagcg ctgagcctcc tcgggcaccg gagcccgctc
     3661 gggctggccg ccggccccca tgacgagctt ccacgtcttc cgggcgatct tgcgcttgag
     3721 cagcgcgtga tgaccggtcc gcagcgtcgg cttgaagatc cagaactcgt ccggatcgag
     3781 ctgcccctgg acgaccgcct cccccagccc gaaggccgcg ttgacgagga ccacgtcggg
     3841 gaacccgctg tcggggtcga gggtgaacat gacgccggag ctgccgcggt cgcacgcaac
     3901 catcctctgg accgcgaccg acaggaacac ggtcagatgg tcgaacccgt tctggatgcg
     3961 atagacgatg gcgcgatcgg tgaagatcga ggcgaagcag cgccggcacg cggtcagcag
     4021 cgcggcctct ccccggacgc ccagaaaact ctcgtgctga cccgcgaagc tcgcctgggg
     4081 aagatcctcc gcggtggcgc tcgagcggac ggcgaccgcg ggctggtcgg ctccggcttc
     4141 gcgcgcgagc gtccggtagg cggagacgat cgcgtcctcc agcccggatg ggagcggggc
     4201 cgactcgacg agccggcgga gctccgcgcc ggccgtggcc agcgcggtca cgtcctcgcc
     4261 ggtgacccct ttgaggaggg cgtcgagccg gtcgcgcagg ccgtcgcggt cgaggagcgc
     4321 ccggtacgcg tccgcggtga ccgcgaaccc atcgggaacg cgaaccccgg cggcgccgag
     4381 ctcgccgtac agttccccca gggaagccgt cttgccgccc acctgggcca cgtctcccag
     4441 ccggatctcc cggagccact tgatgaagac cgtcacggcc ggcgtctccc gcgctgctca
     4501 ttcataccgg agcgcctcga tcggcttgag ccgggatgcc ttgcgcgcgg ggtaatagcc
     4561 gaagaagacg ccgaccgcgg cagcgaagag gaaggcgggc acgacggcgg accatgacac
     4621 cagcacgttc cactggaaca cgaagccgat cgcgtaggac ccgacgatcc cgaccccgat
     4681 gccgatcagg cctcccgcca gcgagacgaa cacggcctcg atcaggaact ggctcaggat
     4741 gtcgcgccgc cgcgccccga ccgcccggcg caggccgatc tcccgcgtcc gctccgtcac
     4801 cgagaccagc atgatgttca tgatgccgat gccgccgacc accagggaga ccgcggccac
     4861 cgccgccagg agcaggctca gcacccggga ggcggcttcc tggctggcca ggatctccga
     4921 caggttccgg atggcgaagt cgtcctcctc gtgcggctgg agacgatggc gctcccggag
     4981 caggtcgcgg acctgcgcct cggcctccgc catgcggccc gggtccgcga ccttgacgag
     5041 aatcgagccc accgagcggg cattggcctg gctcactccc aggaccttct tcttggcggt
     5101 ggagagcggg atcatcagcg tgtcgtcctg gtcctcgccg cgcgtgctct gtcccttccg
     5161 ggccagcact ccgacgaccg tgaagggcac cgccttgatc cgcacggtct ggtcgatcgg
     5221 atcggtgccg ccgaacaggt tctcggcgac gctctgcccc agcacgacca ccttgctcgc
     5281 cccgtccacg tcctccggcg tgag
//
LOCUS       DASUFA010000145         6118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_721280, whole genome shotgun sequence.
ACCESSION   DASUFA010000145 DASUFA010000000
VERSION     DASUFA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721280"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFX87_05145"
     CDS             <1..766
                     /locus_tag="VFX87_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4994329.1"
                     /translation="ARMMETRGRTPNQAHLRRRFLRFCRIAPGWRVVEVGSGSGVVAR
                     DVAALVGPRGHVTGIDRSRVLLAAARRRAREERLDRRVDFRLGEAAALPLRSGRFDCA
                     LAVTVLLHVARPQAMLRELVRVTRPGGVVALQDQDFGTQVLDHPDRELTRRIFEGVTA
                     RLYPDPFSGRTLVRLLVELGLERVRLLADVYQTRRLDPFTHSLLERRAAGAVEFGLVG
                     ARRAAGWLAEIERADAAGHFVFTFNYYGAAGTRPRA"
     gene            789..4838
                     /locus_tag="VFX87_05150"
     CDS             789..4838
                     /locus_tag="VFX87_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4994330.1"
                     /translation="MSAESAGGLESRLRETAALLAVARVASATTDFGEALRLICRELA
                     RLTGAETVSSYVRQADAAALRPVAAYHVPKGALEVLATARLTADEQRGFDAVFQSGQV
                     IWSDDLPNDPRFAIALFRRFPHQSGLVIPLFIDGQVAGAFYLVWWTARRRFEDAELAT
                     LQAIGEQAGVLLRNARLHEALNLRATRLHTLARLNQLVSSSLEPDEVLAGIASAAVEL
                     MQVPAVAVWVVDAAAKRLEIRAVAGGMSGFPRRPVDIGEGDVGWVAAERRQLEVTDIA
                     GDPRAIAPEWWRRSGLKSFLAVPIIFQDLLLGVLVLSGRAPFHFGPDDRELLEIFVSQ
                     SAAAIAKARLFQDIQDRRRLTEDLFALTQAMDRSMDLQERVEIFAAAASRALSFDRLN
                     VWLAEDEGLTLHLMAGSDVGPDVARRIPLRASGGFQAVWDSGITLMVSTDEQLGQVPP
                     LPRELHAHPVLRTRRFAAVPLLVQGRPIGIVSVDNKTSRRPITRRGIAHLELFCQQLA
                     TSVNNARLYGETRQRERDATILLDVTRKLSATLKLDEVIDLISDGVIEALGCGAAGFY
                     RWDAARSGLVLERSRNHAMDFARPLLLRPGEGVTGRAYAERRPVWSGDRLADGSLVYS
                     GPNAAAMARPDAPRAFAAAPILIRDEVFGVLGGSYFQPHAFTEREISLLSSLAAQGAV
                     AIENARLYSVTQQNLAGAALLNAAARTLHRTLDVKRLLPDAAGTLAQTFGARRAGIVL
                     FEERGASPGDVIAWGDWSHESVRALAEPLRRREAPLLIADSAARSDLVTVGLLGPEEA
                     LAAFPVRGRSRVLGGLLLLFPTARALSEAETRLVAAYADQLAMALDNASLFEEAENQK
                     TRLEQVFASTSDGFLVLDLDRRVVALNRQGGTLLGVNPHEVTGRPLARLIETLAGSVD
                     WEGVGGRALTAAVEHAGEPADGDLEVRLPERRTLRWLTTPTRDLLGAVVGTTITLRDV
                     TREREIDRMKTEFVSTVSHELRTPLTSIKGSLHLLLSDDGLVLDETQRHLVDISLKNT
                     DRLIRLINNILDISKMEAGHIHLDLELHRPLDFVEMAVDGIRGFAESRGITVESQVST
                     ELPTVRVDFDRMVQVVTNLLSNAIKFSPERGLVTVGARRSGADLEIWVTDQGRGIAPE
                     DVGRLFRKFQQLDGRTVRAVGGTGLGLAICRGIVEEHGGHIGVDGRPGEGATFTVRLP
                     IPGTAGAAPSGERPEERKEKAPLVLVVDDDPDVRALLRDMLQIGGFRIIEAGRALEGV
                     EMARQRQPDVITMDLMLPDLDGFEAIRLLREQPETRDTPVVVLSAIEVEPGDPRVLGA
                     TVCLAKPFSSADLLKAIRSRLRPRDEVAP"
     gene            4835..6010
                     /locus_tag="VFX87_05155"
     CDS             4835..6010
                     /locus_tag="VFX87_05155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HEX4994331.1"
                     /translation="MTSARILVVDDEADINTILSMTLRRAGYEVISAADGLEALEVIQ
                     LHAPDLILLDVMMPRADGLEALRGIREHEPTAHTPVIMLTAKGALADKMKGFERGADD
                     YVAKPFEPAEVLARVQALLKRTAQSRVVRPLLSVLGDWFTAEHMAQFGRDLEAARDIQ
                     RRLLPPVPARVGGLDAGAVLRSSTVVGGDFFDIFPMGERLGVVVGDVSGKGIPAALLM
                     VMVRTLLREIARDLPQPAEVLTRLNASLCRDMPPSMFVTLVLAALDPGEPGRLVLAGG
                     GHPDPVIVRADRAAAVASLGPAGGTVLGVFPDSTFEQIDLTLAVPGDTLVLLTDGIVE
                     AQSQDGHRPGIPALLPLFERERALSAQALADRLTAEVLLRAGSRLQDDMTVFVLKRS"
BASE COUNT          767 a         2247 c         2246 g          858 t
ORIGIN      
        1 cgcgcggatg atggaaacgc gcgggcgcac tccgaaccag gcgcacctgc gccggcgctt
       61 cctgcgcttc tgccgcatcg cccccggctg gcgcgtggtc gaggtgggct cgggcagcgg
      121 ggtggtcgcg cgcgacgtgg ccgcgctggt cggcccgcgc ggccacgtga ccggcatcga
      181 tcggagccgc gtgctgctcg cggcggcgcg ccgccgggcg cgcgaggagc ggctcgaccg
      241 ccgcgtggac ttccgcctgg gcgaggcggc ggcgctgccg ctccggagcg gccgcttcga
      301 ctgcgccctc gccgtgaccg tgctgctgca cgtggcgcgg ccccaggcga tgctgcgcga
      361 gctggtgcgc gtgacgcggc cggggggcgt ggtggcgctc caggaccagg acttcggcac
      421 gcaggtgctg gatcatcccg accgcgagct gacccgccgg atcttcgagg gcgtgaccgc
      481 gcggctgtat cccgacccgt tctccggccg gaccctggtg cggcttctcg tggagctggg
      541 gctcgagcgc gtgcggctgc tcgccgacgt ctaccagacg cggcggctcg acccgttcac
      601 gcacagcctg ctcgagcggc gcgccgccgg ggcggtcgag ttcgggctgg tcggggcgcg
      661 ccgtgcggcg ggctggctcg ccgagatcga gcgggcggac gcggcgggcc acttcgtctt
      721 caccttcaac tattacggcg cggcgggtac cagacctcgg gcctagtccg cgtatactgg
      781 aagcgagcat gagtgcggag agcgcgggcg ggctcgagtc acggctgcgc gagacggcgg
      841 cattgctcgc ggtggcgcgc gtggcgagcg ccaccaccga cttcggggag gccctccgcc
      901 tcatctgccg cgagctggcg cggctcaccg gcgccgagac cgtctcgtcc tacgtccggc
      961 aggcggacgc cgcggcgctc cggccggtgg cggcctatca cgttcccaag ggcgcgctcg
     1021 aggtgctcgc caccgcccgc ctcaccgccg acgagcagcg cggcttcgac gcggtcttcc
     1081 agagcgggca ggtgatctgg agcgacgacc tcccgaacga cccccgcttc gccatcgccc
     1141 tcttccgccg cttcccgcac cagtccggcc tcgtgatccc gctcttcatc gacgggcagg
     1201 tggcgggcgc gttctacctc gtctggtgga ccgcgcgccg ccggttcgag gacgcggagc
     1261 tggccacgct gcaggccatc ggcgagcagg cgggcgtgct gctccgcaac gcgcggctgc
     1321 acgaggcgct caacctgcgg gccacccgcc tgcacacgct ggcccggctc aaccagctcg
     1381 tctcctcctc gctcgagccc gacgaggtgc tggccggcat cgccagcgcg gcggtggagc
     1441 tcatgcaggt gcccgcggtg gcggtgtggg tggtggacgc ggcggcgaag cgcctcgaga
     1501 tccgcgcggt ggccggcggt atgagcgggt tcccccggcg cccggtggac atcggcgagg
     1561 gcgacgtggg ctgggtggcc gcggagcggc gccagctcga ggtgacggac atcgcgggcg
     1621 acccgcgggc catcgccccg gaatggtggc ggcggagcgg cctcaagagc ttcctcgccg
     1681 tcccgatcat cttccaggac ctgctgctgg gggtgctcgt gctgagcggg cgcgcgccct
     1741 tccacttcgg ccccgacgac cgggagctgc tcgagatctt cgtcagccag tccgccgccg
     1801 ccatcgccaa ggcgcggctc ttccaggaca tccaggatcg gcgccggctc accgaggatc
     1861 tcttcgcgct gacccaggcc atggaccgct ccatggacct gcaggagcgc gtggagatct
     1921 tcgcggccgc cgcgagccgc gcgctcagct tcgatcgcct caacgtgtgg ctggccgagg
     1981 acgagggcct gaccctgcac ctgatggcgg gcagcgacgt gggtcccgac gtcgcgcggc
     2041 ggatcccgct gcgggccagc ggcggcttcc aggcggtgtg ggattcgggc atcaccctca
     2101 tggtgagcac cgacgagcag ctcgggcagg tgccgccgct gccccgcgag ctccacgctc
     2161 acccggtgct gcgcacccgg cgcttcgcgg cggtgccgct cctcgtccag gggcgaccga
     2221 tcggtatcgt cagcgtcgac aacaagacga gccgccgacc catcacgcgg cggggcatcg
     2281 cccacctcga gctcttctgc cagcagctcg ccacctcggt caacaacgca cgcctgtacg
     2341 gcgagacccg ccagcgcgag cgcgacgcaa ccatcctgct cgacgtcacc cggaagctct
     2401 ccgcgacgct gaagctggac gaggtgatcg acctcatctc ggacggcgtc atcgaggccc
     2461 tcggctgcgg cgccgccggc ttctaccggt gggacgccgc acgcagcggc ctcgtgctcg
     2521 agcggagccg caaccacgcg atggacttcg cgcgcccgct cctgctccgg ccgggcgagg
     2581 gcgtgaccgg gcgcgcctac gcggagcgcc ggcccgtgtg gtccggggac cggctcgcgg
     2641 acgggagtct cgtgtactcg gggcccaacg cggcggcgat ggcgcggccc gacgctccgc
     2701 gcgccttcgc ggccgcgccg atcctcatcc gcgacgaggt cttcggcgtg ctgggcggct
     2761 cctacttcca gccgcacgcc ttcaccgagc gtgagatcag ccttctctcg agcctggccg
     2821 cgcagggggc ggtggcgatc gagaacgcgc gtctctactc ggtgacccag cagaacctgg
     2881 cgggcgcggc gctgctcaac gccgccgcgc gcacgctgca ccgcaccctc gacgtgaagc
     2941 ggctgctgcc ggacgcggct ggcacgctcg ctcagacctt cggggcccgg cgcgcgggca
     3001 tcgtgctgtt cgaggagcgg ggcgctagcc cgggcgacgt catcgcgtgg ggtgactgga
     3061 gccacgagtc cgtgcgcgcg ctcgccgagc cgctgcggcg ccgggaggcc ccgttgctga
     3121 tcgcggactc cgcggcccgg agcgacctgg tgaccgtcgg gctcctcggg ccggaggaag
     3181 cgctggccgc cttcccggtg cgcggccgca gccgggtgct gggcgggctg ctcctgctct
     3241 tcccgacagc ccgcgccctc agcgaggccg agacccgcct ggtcgcggcc tacgccgacc
     3301 agctcgcgat ggcgctcgac aacgcctcgc tgttcgagga ggcggagaac cagaagaccc
     3361 gcctcgagca ggtcttcgcc tcgacctccg acggcttcct ggtgctcgat ctcgatcgcc
     3421 gggtggtggc cctcaaccgg cagggcggca ccctgctcgg cgtcaacccg cacgaggtga
     3481 ccgggcggcc cctcgcccgg ctgatcgaga ccctggcggg gtcggtggac tgggaggggg
     3541 tcggcgggcg ggccctcacg gccgcggtcg aacacgcggg cgagccggcg gacggcgacc
     3601 tcgaggtgcg gctccccgag cggcgcacgc tccgctggct gacgaccccg acgcgcgacc
     3661 tgctcggcgc ggtggtcggc accaccatca ccctgcgcga cgtaacgcgg gagcgcgaga
     3721 tcgaccgcat gaagaccgag ttcgtctcga cggtctcgca cgagctgcgc acgccgctca
     3781 cgtccatcaa gggatcgctg cacctgctgc tctccgacga cggcctggtg ctcgacgaga
     3841 cccagcgcca cctggtggac atctcgctga agaacaccga ccggctcatc cggctgatca
     3901 acaacatcct cgacatctcg aagatggagg cgggccacat ccacctcgac ctggagctgc
     3961 accgcccgct ggacttcgtc gagatggcgg tggacgggat ccggggcttc gcggagtcgc
     4021 gcggcatcac ggtcgaatcc caggtgagca ccgagctgcc aacggtgcgg gtggacttcg
     4081 accggatggt gcaggtggtg accaacctgc tctccaacgc gatcaagttc tcgcccgagc
     4141 gcggcctggt cacggtgggc gcccggcgat ccggcgccga cctggagatc tgggtgaccg
     4201 accagggccg gggcatcgcc ccggaggacg tggggcgcct cttccgcaag ttccagcagc
     4261 tcgacggacg caccgtgcgc gcggtgggcg gcaccgggct ggggctcgcg atctgccgcg
     4321 gcatcgtgga ggagcacggc ggccacatcg gggtggacgg ccggcccggc gagggcgcca
     4381 ccttcacggt gcgcctgccg atccccggca ccgcgggcgc cgcgccgagc ggggagcggc
     4441 cggaggagcg caaggagaag gctccgctgg tcctggtggt ggacgacgat cccgacgtgc
     4501 gcgcgctgtt gcgggacatg ctgcagatcg gcgggttccg gatcatcgag gcgggccgcg
     4561 cgctcgaggg cgtggagatg gcgcgccagc gccagcccga cgtgatcacg atggacctca
     4621 tgctgccgga cctggacggc ttcgaggcca tccggctcct gcgcgagcag cccgagaccc
     4681 gcgacacccc ggtggtggtg ctgagcgcga tcgaggtcga gcccggcgat ccgcgcgtgc
     4741 tcggcgccac cgtctgcctg gccaagccgt tcagctcggc cgatctgctc aaggcgatcc
     4801 gctcgcggct gcggccgcgc gacgaagtcg ccccgtgacc tccgcccgca tcctggtggt
     4861 ggacgacgag gccgacatca acacgatcct gagcatgacc ctgcggcgcg cggggtacga
     4921 ggtcatcagc gccgccgacg ggctcgaggc gctcgaggtc atccagctcc acgcccccga
     4981 cctgatcctg ctcgacgtga tgatgccgcg ggcggacggt ctcgaggcgc tgcgcggtat
     5041 ccgggaacac gagcccaccg cgcacacgcc ggtgatcatg ctcaccgcga agggcgcgct
     5101 cgccgacaag atgaagggct tcgagcgggg cgccgacgac tacgtggcca agcccttcga
     5161 gcccgccgag gtcctggccc gcgtgcaggc cctgctcaag cgcaccgccc agtcgagagt
     5221 ggtgcgcccg ctcctcagcg tgctgggcga ctggttcacc gccgagcaca tggcccagtt
     5281 cggccgtgac ctggaggcgg cgcgcgacat ccagcggcgc ctgctgccgc cggtccccgc
     5341 gcgcgtgggc gggctcgatg ccggggcggt cctgcgctcg tccaccgtcg tggggggcga
     5401 cttcttcgac atcttcccga tgggcgagcg cctcggggtg gtggtcggcg acgtgtcggg
     5461 caagggcatt cccgccgcgc tcctcatggt catggtgcgc acgctcctgc gcgagatcgc
     5521 ccgggacctt ccccagcccg cggaggtgct cacccggctg aacgcctccc tctgccggga
     5581 catgccgccc tccatgttcg tgacgctggt gctggccgcg ctcgatccgg gcgagcccgg
     5641 gcggctggtg ctggcggggg gcggccaccc ggacccggtg atcgttcgcg cggaccgcgc
     5701 cgcggcggtg gcctcgctcg gcccggcggg cggcaccgtg ctcggcgtgt tccccgactc
     5761 cacgttcgag cagatcgacc tcaccctggc cgtgcccggc gatacgctcg tgctgctgac
     5821 cgacggcatc gtggaggccc agagccagga cgggcaccgg cccgggatcc cggcgctgct
     5881 gcccctcttc gagcgcgagc gcgcgctgag cgcgcaggcg ctggccgatc gcctgaccgc
     5941 ggaggtgctc ctgcgcgccg gctcgcgcct gcaggacgac atgaccgtct tcgtgctcaa
     6001 gcgctcctga agccctagcg cgccggggcg gccgccttcg cccggatgac gcgcgcctgc
     6061 gcttccatct cggcgccttc cgcttcgcga ttggtctgct tgagcagcgc ggcgtact
//
LOCUS       DASUFA010000146         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1064897, whole genome shotgun sequence.
ACCESSION   DASUFA010000146 DASUFA010000000
VERSION     DASUFA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064897"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFX87_05160"
     CDS             <1..660
                     /locus_tag="VFX87_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX4994332.1"
                     /translation="SLLGVPTALWGALMYAAVGALAALGLTAPRWTWAFYLSAAGVGL
                     SLYLTAVSMLVIQATCAYCLASSVIMVAVLAVLLRRRAVLPGRRKLSALVPGALAAAI
                     LAPLAAAFIYAMPAGGGTGFEGALARHLRETGAVMYGAHWCPHCADQKALFGDAAKDI
                     PYVECAADAVNGRPDLCEKAGVKVFPTWVIGTERREGVQSLSALATLSKFESGTAKPT
                     Q"
     gene            complement(703..2295)
                     /locus_tag="VFX87_05165"
     CDS             complement(703..2295)
                     /locus_tag="VFX87_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX4994333.1"
                     /translation="MADENPPTAPPADAAAEWRPRSSPVLIAISVMLGTFMEVLDTSV
                     ANVALPHIAGSLSATPEQATWVLTSYLISNAIVLPVTAWLSAAFGRKRFLLTCIVIFT
                     LASAACGAAINLEMLILARVIQGIGGGALQPIAQAILLESFAPARRGRAMAAYSMGVI
                     VAPIMGPTLGGWITDSYSWRWIFYINLPVGFLALLMTQTFIEDPPYLQRTTLRRIDYW
                     GLAFMAVSLAALQVVLDKGQQDDWLAAVWIRWVLTLSAATMVIFVVRELTTDEPIVNL
                     RILGDRNFAIGTLLITAMGIVLYATTAMLPLFLQTLLGYPALEAGLAISPRGLGALLA
                     AIAAGRLIGVVDSRLIVSAGFGLLALSGIWLSRLTFDVAWSNIALVTFVNGLANPLIF
                     IALSTMSFATLGREQMGGATGIYNLMRNLGGSVGISMATTLLARRAQVHQNVMVAKMT
                     PYDPVFQQRLEALRKVLTPKVGASQAGQQALGILYRTLVAQATLLAFVDNFRLIAVLA
                     ICCIPLAILLKRARARPAAAAH"
     gene            2343..3665
                     /locus_tag="VFX87_05170"
     CDS             2343..3665
                     /locus_tag="VFX87_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX4994334.1"
                     /translation="MTDLDQEGISGRLRARFSTYQDGPPRRRYLLLAIVMVVLGLAGY
                     AGYLLYYWHYHISTDDAYIAARIAPMSARIPGIVVEVLVNDNQDVKADQVLVRLDPRD
                     YEVAVAQARAAVASAKGDLETAVAGVPLTDESTRSLVQQADAALGATQFGTEMARHDL
                     EERRGVVQAKQAAVAAADASVRAAQADYERSKSDRDRMASLVKANLVAQQEWEHAEAT
                     FKTAEAVLDAARKRFDQARSEVVQAEASLRAQQAAIGQAGRRVDESRASLDNARSQRQ
                     QVRQRQAQVEAGQGRLAQALANLQQAELNLVYCTIRAPMPGRVSRKSVEVGQVVSPGQ
                     SLLAVVDLDDVWVVANYKETELTHVRPGQRATVTVDTYPGKMFKARVDSIQAGSGAVF
                     SLLPPENATGNFVKVVQRVPVKLVFERGENAQHLLVPGMSVVPIIAIR"
BASE COUNT          570 a         1258 c         1376 g          521 t
ORIGIN      
        1 agcctcctgg gcgtcccgac cgcgctctgg ggcgcgctga tgtacgcggc cgtcggcgcg
       61 ttggccgcgc tcgggctgac cgcgccgcgc tggacctggg ccttctacct gtccgccgcg
      121 ggagtgggcc tctcgctcta cctcaccgcc gtctcgatgc tcgtcatcca ggccacctgc
      181 gcctattgcc tggcgtcgag cgtgatcatg gtggccgtcc tggccgtgct gctccgccga
      241 cgcgccgtcc tgccgggccg ccggaagctc tcggcgctcg tgcccggggc cctggccgcg
      301 gcgatcctgg cgccgctcgc cgccgccttc atctacgcga tgcccgcggg cggcgggacc
      361 ggcttcgagg gcgcgctggc gcggcacctg cgcgagacgg gggcggtgat gtacggggcg
      421 cactggtgcc cgcactgcgc cgatcagaag gctctcttcg gcgacgcggc caaggacatt
      481 ccctacgtgg agtgcgccgc ggacgcggtc aacggccgcc ccgacctctg cgagaaggcc
      541 ggcgtgaagg tcttcccgac ctgggtgata ggcaccgagc gccgcgaggg cgtgcaatcg
      601 ctcagcgcgc tggccacctt gtccaagttc gaatcaggaa cggcgaagcc cacccaataa
      661 ccctctcccc tcgggggaga gggcagggtg aggggtgtct tctcagtgcg ccgcggccgc
      721 cggccgcgcc cgcgcgcgct tgagcaggat cgccagcggg atgcagcaga tcgcgagcac
      781 cgcgatcagc cggaagttgt ccacgaaggc cagcagcgtc gcctgcgcca ccagcgtcct
      841 gtagaggatg ccgagcgcct gctgtccggc ctggcttgcg cccaccttgg gggtcaggac
      901 cttccggagc gcttccagcc gctgctggaa caccggatcg tagggcgtca tcttcgccac
      961 catgacgttc tggtgcacct gcgcgcggcg cgccaggagc gtggtggcca tcgagatgcc
     1021 gacgcttccg ccgagattgc gcatcaggtt gtagatgccg gtggccccgc ccatctgctc
     1081 gcgcccgagc gtcgcgaagg acatggtgga gagcgcgatg aagatgagcg ggttggcgag
     1141 gccgttcacg aaggtgacca gcgcgatgtt cgaccacgcc acgtcgaagg tgaggcggct
     1201 cagccagatg ccggacagcg cgagcaggcc gaagccggcc gagacgatca ggcgcgagtc
     1261 cacgaccccg atgaggcggc ccgccgcgat ggcggcgagc agggcaccga ggccgcgcgg
     1321 gctgatcgcg aggccggcct cgagcgcggg gtagccgagc agggtctgga ggaagagcgg
     1381 gagcatcgcg gtggtggcgt agagcacgat gcccatcgcg gtgatcagga gcgtgccgat
     1441 cgcgaagttg cggtcgccca ggatccgcag gttgacgatg ggctcgtccg tcgtcagctc
     1501 gcgcacgacg aagatcacca tcgtggccgc ggagagggtg aggacccagc ggatccacac
     1561 cgcggcgagc cagtcgtcct gctggccctt gtcgagcacg acctggaggg cggccagcga
     1621 gaccgccatg aacgcgagcc cccagtagtc gatgcggcgc agcgtggtgc gctgcaggta
     1681 cggggggtcc tcgatgaagg tctgggtcat caagagcgcg aggaagccta ccggcaggtt
     1741 gatgtagaag atccaccgcc acgagtagct gtcggtgatc cagccgccga gcgtggggcc
     1801 catgatgggc gccacgatca cgcccatcga gtaggcggcc atcgcgcgcc cgcgccgggc
     1861 cggcgcgaag ctctcgagca ggatggcctg ggcgatcggc tggagcgcgc cgccgccgat
     1921 gccctggatc acgcgggcca ggatcagcat ctcgaggttg atggcggccc cgcacgcggc
     1981 ggaggcgagc gtgaagatga cgatgcacgt gagcaggaag cgcttgcggc cgaacgcggc
     2041 gctgagccac gcggtgaccg gcagcacgat ggcgttcgag atgaggtagc tcgtgagcac
     2101 ccaggtggcc tgctcgggcg tggccgagag gctgcccgcg atgtggggca gcgccacgtt
     2161 cgccaccgag gtgtcgagca cctccatgaa ggtgcccagc atcacggaga tcgcgatgag
     2221 caccgggctc gagcggggcc gccactcggc cgcggcgtcc gcgggcgggg cggtgggcgg
     2281 gttctcgtct gccattcgct tgacggggct cggtgccgcc cgccattata cgcatgaatc
     2341 caatgaccga cctggaccag gagggaatat ccgggcggct gcgcgcccgg ttctccacct
     2401 atcaggacgg gccgccgcgc cgccgctacc tgctcctcgc gatcgtgatg gtggtgctcg
     2461 gcctggccgg gtacgcgggc tacctgctct actactggca ctatcatatc tccaccgacg
     2521 acgcctacat cgccgcccgc atcgcgccca tgagcgcgcg cattccgggc atcgtggtcg
     2581 aggtgctcgt caacgacaat caggacgtga aggccgacca ggtgctggtg cgcctcgatc
     2641 cccgcgacta cgaggtggcg gtggcgcagg cgcgcgccgc ggtcgcgagc gccaagggcg
     2701 atctggagac cgcggtcgcg ggcgtgccgc tgaccgatga atccacccgc agcctggtcc
     2761 agcaggcgga cgccgcgctc ggggccaccc agttcggcac cgagatggcg cggcatgatc
     2821 tcgaggagcg ccgcggcgtg gtccaggcca agcaggcggc ggtggcggcg gccgacgctt
     2881 cggtgcgcgc cgcgcaggcc gactacgagc gcagcaagag cgatcgcgac cgcatggcct
     2941 cgctcgtgaa ggcgaacctc gtggcccagc aggagtggga gcacgcggag gcgacgttca
     3001 agaccgcgga ggcggtgctc gacgcggccc gcaagcggtt cgatcaggcg cggagcgagg
     3061 tcgtccaggc cgaggcatcg ctgcgggcgc agcaggccgc catcggccag gcggggcgcc
     3121 gggtggacga gagccgcgcc tcgctcgaca acgcgcgcag ccagcgccag caggtgcggc
     3181 agcgccaggc ccaggtggag gcgggacagg gccggctcgc ccaggccctc gccaacctgc
     3241 aacaggcgga gctgaacctg gtctactgca cgatccgggc gccgatgccg ggccgcgtga
     3301 gccgcaagtc ggtggaggtg gggcaggtgg tgtcgcccgg gcagtcgctt ctcgcggtgg
     3361 tggatctcga cgacgtgtgg gtggtcgcca actacaagga aaccgagctc acccacgtgc
     3421 ggccggggca gcgcgccacc gtcacggtgg acacctaccc gggcaagatg ttcaaggcgc
     3481 gcgtggactc gatccaggcc ggctccggcg cggtgttctc cctgctgcca cccgagaacg
     3541 ccaccggcaa cttcgtgaag gtggtgcagc gcgtgcccgt caagctcgtg ttcgagcgcg
     3601 gggagaatgc ccagcacctc ctggtgcccg gcatgtccgt cgtcccgatc atcgccatcc
     3661 gctagtcccc atgaagatcg atctcctcct gattcccgtg ggcgcccgct acgcgcagat
     3721 gcgcg
//
LOCUS       DASUFA010000147         9224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_489267, whole genome shotgun sequence.
ACCESSION   DASUFA010000147 DASUFA010000000
VERSION     DASUFA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489267"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFX87_05175"
     CDS             complement(<1..267)
                     /locus_tag="VFX87_05175"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX4994335.1"
                     /translation="MAFAFRPAAAPALLLMACLTLAAPRAAAPHAIVLESSPIHDAVL
                     PRAPEQVTLRFNSRIEKRFTRVTLALADQAPVAVAVPAGDTEPTP"
     gene            complement(273..1301)
                     /locus_tag="VFX87_05180"
     CDS             complement(273..1301)
                     /locus_tag="VFX87_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4994336.1"
                     /translation="MRATVDRPGLDSQGRSPLIKIRRVRKGDLSKVRDVIEQAFGDFF
                     ERQVGTRPRQVFGGAQYVHHRWLMEPWGCFVAEEGDGKIVGAAVAVMWGTVGVIGPVA
                     VLTHYQNQDIGQQLLTACQEFFDENKATLQGVATYPYSPKHLIFYQKYGYKPKGLVVV
                     TSKALDRAAVGAPKPAAKPGAAVRRYSTLEEARKKQVMQKVRRITNHFWRGLDVSKEI
                     EIVDGLTLGDTLLLEKGREVIGFAVCHLPPNSEAPHGSAYVKLLAIDSAHRKPEHLQA
                     LLAGVEELAASAQLQRVVVPAYTYYWTAYQTLLERGYHVDFTMVRMKRGKQEDYEDAS
                     DLVLDDWR"
     gene            complement(1298..2188)
                     /locus_tag="VFX87_05185"
     CDS             complement(1298..2188)
                     /locus_tag="VFX87_05185"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX4994337.1"
                     /translation="MAQVVGFIGLGIMGRPMAKNLLKAGYPLVVHSRSQGPVQELVGA
                     GAKGAASPRDVAAQSDLVITMLPNSPDVELVALGKDGLVEGVKRGQLFADMSTISPLV
                     SQKVGKALSAKGVRMLDAPVSGGEKGAIDAALSIMVGGEKADFDAALPVFQALGKTIT
                     HLGPLGAGGFTKLANQIIVAVNLTALGEALTLARKAGLDRALTLKALGGGLAGSKCLE
                     QKTANYVAGTYNPGFKIDLHFKDLGLIMESSRALGVPLPTTAVVQELFNAMRVKGKGG
                     LDHSGVITLLEELAGVPEQS"
     gene            2511..4724
                     /locus_tag="VFX87_05190"
     CDS             2511..4724
                     /locus_tag="VFX87_05190"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009805120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX4994338.1"
                     /translation="MSSASYLTGGGIRVHRTVESIPMANAIEPIVDALDARRGVLLAS
                     SYEYPGRYTRWDMGFVEPPLALVARGRRLRAEALNPRGLVLLPAIAGALQHLDQIEDL
                     ERTDDTVAVTVRPPAPGFAEEDRSRQPSVFSLLRALIELFRHAEEPHLGLYGAFGYDL
                     AFQFEPIRPRLPRPADQRDLVLYLPDELIIVDHRREVAQRRRYDFEAGGRATTGLPRA
                     AAAPRPFSGAAAVPRAGDHEPGAYAAVVRVAREAFKRGDLFEVVPGQTFFEPCPSPPS
                     ELFRRLRERNPAPYGFLMNLGDAEYLVGASPEMYVRVDGERVETCPISGTIARGRDAI
                     ADASQIQALLNSAKDESELTMCTDVDRNDKSRICVPGSVRVIGRRQIEMYARLIHTVD
                     HVEGRLRPGFDALDAFLAHTWAVTVTGAPKTWAMQFIEDHERSPRAWYGGAVGLLGFD
                     GNLNTGLTLRTIRVKDGAAEVRVGATLLYDSDPDAEEEETRLKASAFLDALRRPRGAE
                     PSARVEALRCGAGRRVLLADHQDSFVHTLANYLRQTGAQVTTRRAGFPASVLDELRPD
                     LVVLSPGPGTPSDFDLSGTLKALLERGIPTFGVCLGLQGIVEHLGGELGVLDYPMHGK
                     ASRIQVRGGRLFAGLPGEFTAGRYHSLFALREKLPAALEVTAESDDGIVMAVEHLHLP
                     LAAVQFHPESIMSLEGDVGLRLLRNVVTGLVADRPSGSAPAPRAPGGAPIPDRTR"
     gene            complement(4660..5232)
                     /locus_tag="VFX87_05195"
     CDS             complement(4660..5232)
                     /locus_tag="VFX87_05195"
                     /inference="COORDINATES: protein motif:HMM:NF022607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HEX4994339.1"
                     /translation="MSRRVRTWGVPAAVAVLAALAWLYAVSTPRYALYRLGVAMQRHD
                     VAAAERYFDVERIADRATEVIVADYLARQPEPTTAAESLGRQVAAGMAKRRVRPQVAT
                     RVRLELRHSVERAGIQATALVLPVGAVAVFRAFEVASAGPDVWVTYRDPAQGAVRFRM
                     SRRAEGGWQISEFDPEWVRRRAHEEPARIR"
     gene            5331..5822
                     /locus_tag="VFX87_05200"
     CDS             5331..5822
                     /locus_tag="VFX87_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX4994340.1"
                     /translation="MNNIYASEGSEAGRRRREILALVGRGGVRSQEELQRRLRRRGIA
                     VAQPTLSRDLKTLGLAKTPTGYVTTPAGAFVPAETRRGALDRAVGEFVLSVRAAASLV
                     VVRTPPAGAHPLARALDEAGLPEVVGTIAGDDTVFVAATGPATARLLERRLRAPLGGR
                     KGA"
     gene            5828..6307
                     /locus_tag="VFX87_05205"
     CDS             5828..6307
                     /locus_tag="VFX87_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4994341.1"
                     /translation="MHHERHDGNILVTTDPARLDLDTIHDFLSRTSYWATGIPREVME
                     RSIRHSICFGAFDRGRQVGFARVISDRATFAYVCDVFVLDSHRGRGVGNRIMACITSH
                     PDLQNLRLWTLFTRDAHGLYRQHGFRDARHPDRLMERRTERPYALSAAGHSHDERTA"
     gene            6304..7470
                     /locus_tag="VFX87_05210"
     CDS             6304..7470
                     /locus_tag="VFX87_05210"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX4994342.1"
                     /translation="MSTDLTAVMKVADRPPVVMVEGHGSWLQDEDGKAYLDFVQGWAV
                     NCLGHSPSVMTEAIAFQAGRLINCSPAYYNAEMARLARLLADRAGLAQVFFCNSGAEA
                     NEGAIKLARKWGARHRDGAHEIITMDHGFHGRTLATMAASGKPAWEALFEPKVRGFTK
                     VPLGDLDAVKRAITPRTVAVMLEPIQGEAGVYPAGDDFLRGLRAVTTEAGLLLILDEI
                     QTGIGRTGRLFAFEHAGVEPDILTLGKGLGGGVPLAALLAAEPVCCFEYGDQGGTFNG
                     SPLMTAIGSAVVETVSRPAFLADVTAKGEYLADRLRELSATLGHGEVRGRGLLLALGL
                     RGKDAAKVARSAMDHGLLINAPRPDTLRFMPALNVTRDEIDRMLALLESVLREA"
     gene            complement(7472..8449)
                     /locus_tag="VFX87_05215"
     CDS             complement(7472..8449)
                     /locus_tag="VFX87_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994343.1"
                     /translation="MMGRRAWLAGSLALLATPRVALAQSPARVFRIGLLGGSSPTSPE
                     SHIWAAFFQELRNLGYVEGQNIAFEGRYYGDRVEDLPGFAAELVRLQVDVIVAAAAPA
                     PEAAKHATATIPIVMANHADPVGSGLVASIARPGGNVTGLSLPAVELRLKQLQLLKEI
                     LPGLSRVAFLRNPTIPLDVRELETAARSLMIRVQVVEARTPSDFRSAFAAATKERAGA
                     LIVLAGSLFFAHRVRLAELAAQSRLPTVYLLREHVDAGGFMTYGVDIRENFRRAAGYV
                     DRILKGTRPSDLPVEQPTKFELAINLKAAKALGLTIPPSVLARADRLVE"
     gene            complement(8470..8739)
                     /locus_tag="VFX87_05220"
     CDS             complement(8470..8739)
                     /locus_tag="VFX87_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994344.1"
                     /translation="MTKHAGGDGTGSKLFDEQIGRGDTPRTVLRRFTARFPELDSALW
                     SADHSELGSHIEVLVNDEVLGVGYELDTPLLGGERITLLGQFMGG"
     gene            complement(8832..>9224)
                     /locus_tag="VFX87_05225"
     CDS             complement(8832..>9224)
                     /locus_tag="VFX87_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4994345.1"
                     /translation="RRHFEDSLGGCSFTFRTRMANLASALSAATGWDYRLKDAVRFGN
                     RTAAILRAFNLRCGIGTEVEYPSARYGSQPVDGPAKEHDVRKQWDRMLEVWYETVDYD
                     RKTGKPKKELLHSLDLDWLAKDLYGKKK"
BASE COUNT         1296 a         3347 c         3211 g         1370 t
ORIGIN      
        1 gggcgtcggc tccgtgtcgc ctgcgggcac cgccaccgcc accggcgcct gatccgcgag
       61 ggccagcgtc acccgcgtga agcgcttctc gatccggcta ttgaagcgga gcgtgacctg
      121 ctccggagcg cgcggcagca ccgcgtcgtg gatcggggag gactcgagca cgatggcgtg
      181 cggagccgcc gcgcggggcg cggcgagcgt gagacaggcc atcagcagca gggccggcgc
      241 ggcggccggc cggaacgcga atgccacgcg gatcagcgcc agtcgtccag caccagatcg
      301 ctggcgtctt cgtagtcctc ctgcttgccc cgcttcatgc ggaccatggt gaagtccacg
      361 tgatagccgc gctcgagcag cgtctggtag gcggtccagt agtaggtgta ggcgggcacg
      421 acgacccgct ggagctgggc ggaggccgcc agctcttcca ccccggcgag cagggcctgc
      481 aggtgctcgg gcttgcgatg ggcgctgtcg atcgccagca gcttgacgta ggcgctgccg
      541 tggggcgcct cgctgttggg cggcaggtgg cagaccgcga agccgatcac ctcgcgaccc
      601 ttctcgagca ggagggtgtc gcccagggtg aggccgtcga cgatctcgat ctccttgctc
      661 acatcgagcc cgcgccagaa gtgattggtg atgcgccgca ccttctgcat cacctgcttc
      721 ttgcgcgctt cctcgagcgt cgagtatcga cgcaccgcgg ccccgggctt ggccgcgggc
      781 ttcggcgcgc cgaccgccgc ccgatcgagc gccttgctgg tcaccaccac caggcccttc
      841 ggcttgtagc cgtacttctg gtagaagatc aggtgcttgg ggctgtaggg ataggtcgcc
      901 accccctgca gcgtcgcctt gttctcgtcg aagaactcct ggcaggcggt gaggagctgc
      961 tggccgatgt cctggttctg gtagtgggtc agcaccgcca ccggaccgat cacgcccacc
     1021 gtgccccaca tcaccgcgac ggcggcgccc acgatcttgc cgtcgccctc ctcggccacg
     1081 aagcagcccc acggctccat gagccagcgg tggtgcacgt actgggcccc tccgaacacc
     1141 tggcgcggcc gcgtgcccac ctgccgctcg aagaaatcgc cgaaggcctg ctcgatgacg
     1201 tcgcggacct tggagagatc gcccttccgg acgcggcgga tcttgatgag gggcgagcgg
     1261 ccctgcgagt cgaggcccgg gcgatcgacg gtcgccctca tgactgctcc ggcacgcccg
     1321 ccagctcctc gagcagcgtg atcacgccgg agtgatcgag acctcccttg cccttgaccc
     1381 gcatggcgtt gaacagctcc tgcaccaccg cggtggtcgg caggggcacg ccgagggcgc
     1441 gggacgactc catgatgagg cccaggtcct tgaagtgcag atcgatcttg aagcccgggt
     1501 tgtaggtccc cgccacgtag ttggccgtct tctgctcgag gcacttcgag cccgccagcc
     1561 cgccgcccag cgccttgagc gtgagcgcgc gatcgagccc ggccttgcgc gccagcgtca
     1621 gcgcctcacc cagcgcggtg agattcaccg ccacgatgat ctggttggcg agcttggtga
     1681 agccgcccgc gcccaacggc cccaggtgcg tgatcgtctt gccgagtgcc tggaacaccg
     1741 ggagcgccgc gtcgaagtcg gccttctcgc cgccgaccat gatggacagc gcggcgtcga
     1801 tggcgccctt ctcgccgccc gagaccggcg cgtcgagcat gcgcacgcct ttggccgaga
     1861 gcgctttgcc gaccttctgc gagaccagcg gggagatcgt ggacatgtcg gcgaagagct
     1921 ggccgcgctt gacgccctcg accaggccgt ccttgccgag cgccaccagc tccacgtccg
     1981 gggagttcgg cagcatcgtg atcaccaggt cggactgggc cgcgacgtcc cgggggctgg
     2041 cggccccctt ggcccccgcc ccgacgagct cctggaccgg cccctggctc cggctgtgga
     2101 caaccagtgg atagccggcc tttagcaagt tcttggccat gggacggccc atgattccaa
     2161 gcccaatgaa tccaacgact tgcgccatgg taacctcgtg gggaccgaat tgggaggagc
     2221 tgcggcggcc cggaggttgg agtaacacag acgcgcgcag tgagtcaagt cattccccag
     2281 cccattgaca ggcggcacgg ttcctgcgac tcttccgctc gtgaattcga cgcgcgcggc
     2341 cgtggcctgg tcctggtggt ggccctgccc ctgaggggcg ggcccgccgg ctcgagcgcg
     2401 tgcgcgagtg acctaggcga gaccggagcc gcccccaggc tggggcggct tctgcgtgct
     2461 ctcgccccca cctgggagcc ggctcctcgg agagagagga gattccggcc atgagcagtg
     2521 ccagctacct cacgggtggg ggcatccgcg tccaccgcac ggtggagtcc atcccgatgg
     2581 cgaatgccat cgagccgatc gtggacgccc tcgacgcgcg acgcggcgtg ctgctcgcgt
     2641 cgagctacga gtaccccggc cgctacacgc ggtgggacat ggggttcgtg gagccgccgc
     2701 tcgcgctcgt ggcccgcggc cgccggctcc gcgccgaggc gctgaacccc cgcggcctgg
     2761 tcctgctccc ggccatcgcc ggggcgctcc agcacctcga tcagatcgag gacctcgagc
     2821 ggacggacga cacggtggcg gtgaccgtgc gcccgcccgc gcctggcttc gcggaagagg
     2881 atcgcagccg ccagccctcg gtgttctcgc tgctccgcgc gctgatcgag ctcttccggc
     2941 acgccgagga gccgcacctc gggctctatg gcgccttcgg ttacgatctc gcgttccagt
     3001 tcgagccgat ccggccgcgc ctaccgcgcc cggccgacca gcgcgatctg gtgctctacc
     3061 tgccggacga gctgatcatc gtggatcacc gccgcgaggt ggcccagcgc cgccgctacg
     3121 acttcgaggc gggcggtcgc gccaccaccg gcctgccccg cgcggcggcg gcaccacgcc
     3181 cgttctcggg tgccgccgcg gtgccgcgcg cgggcgacca cgagcccggc gcgtacgcgg
     3241 ccgtcgtccg cgtggcccgc gaggccttca agcgcggcga cctcttcgag gtggtccccg
     3301 gccagacctt cttcgagccc tgcccctcgc cgccgtcgga gctcttccgt cgcctccgcg
     3361 agcgcaaccc cgcgccctac gggttcctga tgaacctcgg ggacgcggag tacctggtgg
     3421 gcgcttctcc cgagatgtac gtgcgcgtgg acggcgagcg ggtggagacc tgcccgatct
     3481 cgggcacgat cgcgcgcggc cgcgacgcca tcgccgacgc gagccagatc caggcgctgc
     3541 tcaactcggc caaggacgag tcggagctca cgatgtgcac cgacgtggac cgcaacgaca
     3601 agtcgcgcat ctgcgtcccg ggcagcgtgc gggtgatcgg ccgccgccag atcgagatgt
     3661 acgcgcgcct catccacacc gtggatcacg tggaggggcg cctccggccc ggcttcgacg
     3721 cgctcgacgc cttcctcgcg cacacgtggg cggtcaccgt caccggggcg ccgaagacgt
     3781 gggccatgca gttcatcgag gaccacgagc ggtcgccccg cgcctggtac ggcggcgcgg
     3841 tcgggctcct cggcttcgac ggcaatctca ataccgggct caccctgcgc acgatccggg
     3901 tcaaggacgg cgccgccgag gtgcgcgtcg gggccaccct cctctacgac tccgaccccg
     3961 acgccgagga ggaggagacg cggctgaagg cctccgcctt tctcgatgcc ctccggcgcc
     4021 cccgcggcgc ggagccgagc gcgcgggtgg aggcgctgcg ctgcggcgcg ggccgccgcg
     4081 tgctcctggc ggaccaccag gattcgttcg tccacacgct ggcgaactat ctccgccaga
     4141 ccggggctca ggtgaccacg cgacgggccg ggttcccggc gagcgtgctc gacgagctcc
     4201 ggcccgatct ggtggtgctc tcgcccggcc ccggcacgcc cagcgatttc gatctgtcgg
     4261 gcacgctgaa ggcgctgctc gaacgcggga ttccaacctt cggggtctgc ctcgggttgc
     4321 agggcatcgt ggagcatctg gggggcgagc tcggcgtgct cgactacccg atgcacggca
     4381 aggcctcgcg catccaggtc cgcggcgggc gactcttcgc gggcctgccg ggcgagttca
     4441 ccgcgggccg ctaccactcc ctcttcgcgc tccgcgagaa gctgccggcc gcgctggagg
     4501 tcacggccga gagcgacgac ggcatcgtga tggcggtgga gcacctccat ctgccgctgg
     4561 ccgcggtgca gttccatccg gagtccatca tgtcgctcga gggcgacgtc gggctgcggc
     4621 tgctccggaa cgtggtcacc ggcctggtcg cggaccgtcc tagcggatcc gcgccggctc
     4681 ctcgtgcgcc cggcggcgca cccattccgg atcgaactcg ctgatctgcc agccgccctc
     4741 ggcccggcgg ctcatccgga agcggaccgc gccctgggcg ggatcgcgat aggtgaccca
     4801 gacgtcggga ccagcgctgg cgacctcgaa ggcgcggaag accgcgaccg ctcccaccgg
     4861 gagcacgagg gcggtcgcct ggatgccggc ccgctccacg ctgtgccgga gctcgagacg
     4921 cacccgcgtc gccacctgcg gccgcacgcg gcgcttggcc atgccggccg cgacctggcg
     4981 cccgagcgac tcggcggcgg tggtgggctc gggctggcgc gcgaggtagt cggccacgat
     5041 cacctcggtg gcgcggtcgg cgatccgctc cacgtcgaag tagcgctcgg ccgccgccac
     5101 gtcgtgccgc tgcatggcca cgcccaggcg gtagagcgcg tagcgggggg tggacacggc
     5161 gtagagccag gcgagcgcgg ccagcacggc caccgcggcc ggcacgcccc atgtccgcac
     5221 gcgtctcgac atcgcccggc cacgctaccc tagccgccgc cgcccgcgca accgcggccc
     5281 gggcggatgg ggcttgaccg ccgggccctc cggatgtatt aatattcagt atgaacaata
     5341 tatatgcgtc ggaaggcagc gaggcgggcc ggcggcggcg cgagatcctc gcccttgtcg
     5401 gccggggcgg ggtgcgctcg caggaggaac tgcagcgccg gctgcggcgc cgcggcatcg
     5461 cggtcgccca gccgaccctc tcccgcgacc tcaagacgct cgggctcgcc aagactccga
     5521 ccggctacgt gaccacgccc gcgggtgcct tcgtgcccgc cgagacccgg cgcggcgcgc
     5581 tcgaccgggc ggtgggcgag ttcgtgctct cggtgcgggc cgccgcctcg ctcgtcgtgg
     5641 tgcgcacgcc gcccgcgggc gcccatcccc tggcccgcgc cctcgacgag gccggcctgc
     5701 ccgaggtggt cggcaccatc gcgggcgacg acacggtgtt cgtggccgcc accgggccgg
     5761 cgaccgcccg gcttctcgag cggcgcctgc gcgcccccct cggcgggagg aaaggagcgt
     5821 gagcccgatg caccacgagc ggcacgacgg caacatcctg gtcaccacgg atccggcgcg
     5881 gctcgacctg gacacgatcc acgacttcct ctcccggacg tcctactggg ccaccgggat
     5941 tccccgcgag gtcatggagc ggtccatccg ccactcgatc tgcttcggcg ccttcgaccg
     6001 cggccggcag gtggggttcg cccgcgtcat cagcgaccgc gcgacgttcg cctacgtctg
     6061 cgacgtcttc gtgctggaca gccaccgcgg ccggggtgtg ggcaatcgga tcatggcctg
     6121 catcacgagc cacccggacc tacagaacct gcgcctctgg accttgttca cgcgggacgc
     6181 tcacgggctc taccgccagc acggcttccg cgacgcgcgc cacccagacc ggctcatgga
     6241 gcggcgcacc gagcgcccct acgccttgag cgcggccggc cattcccacg acgagaggac
     6301 agcatgagca ccgatctgac ggcggtgatg aaggtggcgg accggccccc tgtggtcatg
     6361 gtggaggggc acggatcttg gctgcaggac gaggacggca aggcctatct cgacttcgtg
     6421 cagggctggg cggtgaactg cctcgggcac tccccgtccg tcatgaccga ggccatcgcc
     6481 ttccaggccg ggcggctgat caactgcagc cccgcctact acaacgcgga gatggcgcgg
     6541 ctggcccgcc tgctcgccga tcgcgccggg ctcgcgcagg tgttcttctg caacagcggc
     6601 gcggaggcca acgagggcgc gatcaagctg gcgcggaagt ggggcgcccg ccaccgcgac
     6661 ggcgcgcacg agatcatcac catggaccac ggcttccacg gccgcacgct cgccaccatg
     6721 gccgcctccg gcaagcccgc gtgggaggcg ctcttcgagc ccaaggtgag gggcttcacc
     6781 aaggtgccgc tcggcgacct ggacgcggtg aagcgcgcga tcactccccg gacggtggcg
     6841 gtcatgctcg agcccatcca gggcgaggcc ggcgtctatc ccgccggcga cgacttcctg
     6901 cgcgggctcc gggcggtcac gacggaagcc gggttgctgc tgatcctcga cgagatccag
     6961 accggcatcg ggcggacggg acggctcttc gccttcgagc acgcgggcgt cgagcccgac
     7021 atcctgaccc tcggcaaggg actgggcggc ggcgtgccgc tggcggccct gctggccgcc
     7081 gagcccgtgt gctgcttcga gtacggcgac cagggcggca ccttcaacgg ctccccgctc
     7141 atgaccgcca tcggcagcgc ggtggtcgag accgtgagcc gccccgcgtt cctggccgac
     7201 gtgacggcca agggcgagta cctggcggat cggctgcgcg agctctccgc cacgctcggc
     7261 cacggcgagg tgcgcgggcg cggcctgctg ctcgcgctcg ggctccgggg caaggacgcg
     7321 gccaaggtcg cgcggagcgc catggatcac ggcctgctca tcaacgcgcc gcggccggac
     7381 accctgcgct tcatgcccgc gctcaacgtc acccgcgatg agatcgaccg gatgctcgcc
     7441 ctgctcgagt cggttctccg cgaggcctag gctattcgac gaggcggtcc gcccgcgcga
     7501 gcaccgatgg ggggatcgtg agcccgagcg ccttcgccgc cttgaggttg atcgccagct
     7561 cgaacttcgt tggctgctcg accgggaggt cgctcggccg ggttcccttc aggatccggt
     7621 caacgtaccc ggccgcccga cgaaagttct cgcgaatgtc gaccccgtac gtcatgaatc
     7681 cacccgcgtc cacgtgctct ctcagcaagt acaccgtcgg caggcggctc tgagccgcca
     7741 gctccgcgag ccgcactcga tgggcgaaga acaaggagcc ggcgaggacg atgagggcgc
     7801 cggcccgctc cttcgtcgcc gccgcgaagg cactccggaa atcgctcggc gtccgtgcct
     7861 ccacgacctg gacccggatc atcagcgatc gcgccgcggt ctccagctct ctcacgtcga
     7921 gggggatggt tggattccgg aggaacgcca cgcggctgag tccgggcagg atctccttga
     7981 gcagctggag ctgcttgagc cgcagctcca cagcggggag ggatagtccc gtcacgtttc
     8041 cccccggtct cgcgatgctc gccaccaggc cactcccgac cggatcggca tggttcgcca
     8101 tgacgatcgg gatcgtggcg gtggcgtgct tggccgcctc gggtgccggg gcagccgcgg
     8161 cgacgatcac gtcgacctgc agccgaacca gctcggccgc gaagccggga aggtcctcga
     8221 cccgatctcc gtagtatcgg ccctcgaagg cgatgttctg gccctcgacg tacccgaggt
     8281 tccgcagctc ctggaagaat gcggcccaga tgtgggactc cgggctggtc ggcgaggacc
     8341 caccgagaag gccgattcga aacaccctcg ccggcgactg cgccagcgcg acccgcgggg
     8401 tggcgagcag ggcgagggag cctgccagcc atgcgcgccg acccatcatc cgggcaccgt
     8461 ccccgccacc taccctccca tgaactgccc gagcagcgtg atgcgctcgc cgcccagcag
     8521 cggcgtgtcc agctcgtagc cgacgccgag gacctcgtcg ttcaccagga cctcgatgtg
     8581 ggagccgagc tcggagtggt cggcactcca gagggcggag tcgagctcgg ggaagcgggc
     8641 ggtgaagcgg cggagcacgg tgcgcggagt gtcccctcgc ccgatctgct cgtcgaagag
     8701 cttgctaccg gtcccgtcgc cgccggcgtg cttggtcacc cacgtcgtga cttctatctg
     8761 cgctcgggac ggtatatccg ctcggctcgc cttcggctgc gcctcgccag gcaactgccc
     8821 ctccccgcgc cttacttctt cttcccgtag aggtccttgg cgagccagtc gaggtcgagc
     8881 gagtgcagga gctctttctt gggcttgccg gtcttgcggt cgtagtcgac ggtctcgtac
     8941 cagacctcga gcatgcgatc ccactgcttg cggacgtcgt gctccttggc agggccgtcc
     9001 accggctgcg agccgtaacg ggcggaggga tactcgacct cggtgccgat gccgcagcgc
     9061 aggttgaagg cgcgcaggat cgcggcggtg cgattgccga agcgcaccgc gtccttcagc
     9121 cgatagtccc agccggtcgc ggccgatagc gcgctcgcca ggttggccat acgggtgcgg
     9181 aaggtgaacg agcagccgcc cagggaatcc tcgaagtggc gccg
//
LOCUS       DASUFA010000148         7753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1402485, whole genome shotgun sequence.
ACCESSION   DASUFA010000148 DASUFA010000000
VERSION     DASUFA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402485"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFX87_05230"
     CDS             complement(<1..391)
                     /locus_tag="VFX87_05230"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4994346.1"
                     /translation="MIRSTLDVTKHTPSARALVAAEHPLGAEVGAGILARGGNAVDAA
                     VATAFAMTVVEPFMSSVAGGGTMLVHLAKRGATVALDFNVQAPAACHESCYELGEGVA
                     QALFPWRRVVGDANNVGPKSVAIPGSVA"
     gene            complement(388..1008)
                     /locus_tag="VFX87_05235"
     CDS             complement(388..1008)
                     /locus_tag="VFX87_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994347.1"
                     /translation="MPSAALTWIEPGASATLIWYGAAASQPGRGRLYVVAGPILEQPP
                     ASPYFILAAEEDGDFAARLYRGQVTLPELRAFLSRCRVARGALVDDQQYVATDEELPV
                     LALLDDWRGWPGAPVLPYLDDINAFMPAAAPLYVTAEAHAAAQREPEQFGTAWVCDEC
                     GEAEDAGVFFWTAHRGPLVRVCFLIHNDAGVWTCRLHPFEFAKETA"
     gene            complement(1044..1550)
                     /locus_tag="VFX87_05240"
     CDS             complement(1044..1550)
                     /locus_tag="VFX87_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994348.1"
                     /translation="MSRLTRSSSWLAAAALLTAGCAATEPVLERSTRGPNAEEFFIMQ
                     SFAVNGRAPNFDEKRVWQDQMDEKVFKYLREHPELENTSRYSEFRFWRQVTNGSTPGE
                     VKILLGEPRERTIDPALMASLGEQHWQAVRTTAKEAWVYPLGWVVFFDDKGVVDLLRR
                     VSPLDREE"
     gene            complement(1588..4170)
                     /locus_tag="VFX87_05245"
     CDS             complement(1588..4170)
                     /locus_tag="VFX87_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4994349.1"
                     /translation="MAEQLLESFRARYQFPLDDFQLRAIAAILDGKSVIVSAPTGSGK
                     TLVAEFAIHAALHRGRRIAYTTPLKALSNQKYADFVRQWGAEQVGILTGDVKVNSRAP
                     VVVMTTEILRNMFYSGGLEGLDTVVLDECHYMGDEGRGTVWEEIIVNCPKDVALVALS
                     ATVRNVDEIADWISLVHRPIVAITHPHRPVPLQYLVADLSGEIHTYDAVRQGKVRLLG
                     EEREGGYNDRGRWYTRRVVDPTVMLDELESRGWLPAIYFIFSRAGCERAMETVLAEGK
                     PLLTREQQRQVDEAIREQVTENPTIAESPLNQTIFQALGMGVGLHHAGILPTVKRFIE
                     LLFERGLCKVVFATETMSLGIHMPAKSVMLQSLTKRTDRGFRSLTHNELTQMAGRAGR
                     RGIDPEGKCVMALDVRDGLDDMKRVVNGPPEPIASQFKLGYGSVALLLSTGNDLATIR
                     RTVESSFGQYQNLKRIKSLETDVRSLEMAVADGQRFDAPCGDFARIGRYRQARSEVEV
                     ARMARGRGGRRGRAAADAEPGRLALVRRKSGATLGLIVGVDRRRHRDLIQVLLAHGGT
                     VQMKEGNVKRVFWQTPPLHVPRDWEHRTRGLTEQMARLSVPELIEREKSHGPEGALAA
                     IECHRCPWGSQSRCEQAWKGLDGVERQLAQKREMLDAFRNAYWQEFLRVVEVLERFGA
                     VQDAKLLAKGQLIAGLRHDNELLVAEVVDHGVLRDTTLAEAAALCSGLIEESRSGDAA
                     VARLFLKKRPKIKRKLQEMESIANTVLEVQRRHGLPMPVSISTGFMPSVFRWASGEDD
                     WSAIVEESFGGHEGDLIRAMRRLIDVLRQLAESPEVEPVVAGLLSRASRVIDRGIVLE
                     SALI"
     gene            4268..5026
                     /locus_tag="VFX87_05250"
     CDS             4268..5026
                     /locus_tag="VFX87_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994350.1"
                     /translation="MKLTGKVALITNVSHFMGPAITEEFCREGASVALHDRDEAAIKP
                     YALIAERLGRDVLSLGGDLSRSGEAERAVQAVVARFGRLDILVNNNAHPPSGSPVEEI
                     TDAAWREMMARLLDEPFFCLRAALRVMRPAGRGKIINMSSAAAFPGLPNYAAYTAARA
                     GVNGLTKAVGREVAGAGIQVNAIAQNYVENPTYFPPALTGDPARLARMVKNIPAGRLA
                     RSEESARLAVYLASEDADFFIGQVIPFAGGWVTP"
     gene            5023..5559
                     /locus_tag="VFX87_05255"
     CDS             5023..5559
                     /locus_tag="VFX87_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4994351.1"
                     /translation="MIVYHVVVKTGDDYLQTREPHRKAHLERLMGLRAQGLVIGGGPT
                     PDGRGADLFYRVQQPADLRRLVEEDPYFTGGVWPGYTPRSFAQFLEPWESPPLVTDGS
                     RRVTLVEGLAPDVEMASFALIEARGSGRMAFGGFFPGGETLAVMRGDDAAKALDELRA
                     TGFWDEASLRARPLLYAL"
     gene            5887..7698
                     /locus_tag="VFX87_05260"
     CDS             5887..7698
                     /locus_tag="VFX87_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX4994352.1"
                     /translation="MLDYLGVDYPEAVKDGKVADQGEYDEQLEFAASVLATIGELPAR
                     PERAGLEAAAARLAGAIRDKRPGAEVAAIAGELRGRIIEVYQVRVSPRHAPDLASAPA
                     DFATHCAVCHGAAGRGDGPTARGLTPPPADLTDAARQAQLSVFGLYNTITLGIKGTAM
                     TGFPALSDAQRWGLAFHVSTLAGAGDERLRGAALWKGGTGRRELGDLRALVMATPREV
                     TARLGGDGAAVLAYLRGEPGAVAAGGESALDRSARLLDESLEAYRKGDAARAHQLAVT
                     GYLEGFELVETPLGAVDGALKTRVEAEMMRYRSMLQAGAPVDAVAAQARAIQALLVTA
                     RQRLDAARLSPATIFTSALVILLREGLEAILVLAALVALLIKSGRREALRYVHAGWIA
                     ALALGGLTWLAASYLVTVTGAGREITEGVTALLAAAMLLYVGFWMHRHAHAARWKAFI
                     ETRVASALSGGTMWALASIAFLAVYREAFETVLFYQALWLEAGPDGRLAVGAGFAAAV
                     VALVVLAWLILKVGLRLPVGWFFGGGAILMAVLAVVLAGKGIAALQEAGTLPIGPLDL
                     PAIPSLGLYPTWQGVLTQVAVVLLIVGAFAYSRRREA"
BASE COUNT         1065 a         2824 c         2739 g         1125 t
ORIGIN      
        1 cggccaccga gcccggaatc gccaccgact tcgggccgac gttgttggcg tcgcccacca
       61 cgcgccgcca cggaaacagg gcctgcgcca cgccctcccc cagctcgtag cagctctcgt
      121 ggcacgccgc gggggcctga acgttgaagt cgagcgcgac cgtcgcgccg cgcttggcca
      181 ggtgcaccag catggtgccg ccgcccgcca ccgaggacat gaagggctcc accaccgtca
      241 tcgcgaacgc ggtggccacc gccgcgtcca ctgcgttgcc gccacgggcc aggatgcccg
      301 cgcccacctc ggccccgagc ggatgctcgg cggccaccag ggcgcgcgcg ctcggggtgt
      361 gcttggtcac gtcgagggtg gagcggatca tgcggtctcc ttcgcgaact cgaacgggtg
      421 gagccggcag gtccagaccc cggcgtcgtt gtggatgaga aagcacacgc gcaccagcgg
      481 cccgcggtgc gcggtccaga agaagacgcc cgcgtcctcg gcctccccgc actcgtcgca
      541 gacccacgcg gtgccgaact gctcgggctc ccgctgcgcg gccgcgtggg cctccgcggt
      601 cacgtagagc ggcgcggcgg cgggcatgaa ggcgttgatg tcgtcgagat acggcaacac
      661 cggggccccc ggccacccgc gccaatcgtc gagcagggcg agcaccggca gctcctcatc
      721 cgtggcgacg tactgctggt cgtccaccag cgcgccgcgg gcgacacggc accgggacag
      781 gaaggcacgg agctcgggca gcgtcacctg cccgcgatag agccgggccg cgaagtcgcc
      841 gtcttcctcc gcggcgagga tgaagtaggg ggacgccggg ggctgctcca ggatggggcc
      901 ggccaccacg tagagccggc cgcggccggg ctgggatgcc gccgcgccgt accagatgag
      961 ggtggcgctc gctcccggct cgatccacgt gagggcggca ctcggcatgg gcgtcgtctg
     1021 agacgcccaa gtgtagcacg cagctattct tcgcgatcga gtggcgagac ccggcggagc
     1081 aggtcgacca cgcccttgtc gtcgaagaag acgacccagc cgagcgggta cacccaggct
     1141 tccttcgcgg tggtccggac cgcctgccag tgctgctcgc cgagcgaggc catcagggcg
     1201 gggtcgatgg tccgctcgcg gggctccccc agcaggatct tcacctcgcc gggggtgctg
     1261 ccgttcgtca cctggcgcca gaagcggaac tcgctgtagc gcgaggtgtt ctcgagctcc
     1321 ggatgctcgc gcagatactt gaagaccttc tcgtccatct ggtcctgcca gacacgcttc
     1381 tcgtcgaagt tgggggcccg gccgttgacc gcgaatgact gcatgatgaa gaactcctcg
     1441 gcgttgggtc cccgggtgct gcgctcgagc acgggctcgg tcgcggcgca cccggcggtc
     1501 aggagggccg ccgcggccag ccaggaggag gagcgggtca gtcggctcat gccgatctca
     1561 ttatatatac cgggtgccgc cagcccctca aatcagggcg gactccagca cgatcccgcg
     1621 atcgatgacc cgggacgccc ggctcaggag gccggccacc accggctcga cctcgggcga
     1681 ctcggcgagc tggcgcagga cgtcgatgag ccgccgcatg gcgcggatca ggtccccctc
     1741 gtggccgccg aaggactcct ccacgatcgc cgaccagtcg tcctcgccgg aggcccagcg
     1801 gaacacggag ggcatgaagc cggtggagat ggagacaggc atcggcagcc cgtgccggcg
     1861 ctggacctcg aggacggtgt tcgcgatgga ctccatctcc tgcagcttgc gcttgatctt
     1921 gggccgcttc ttgaggaaga gccgcgccac cgccgcgtcc ccggagcgcg actcctcgat
     1981 gaggcccgag cagagcgccg ccgcctccgc gagggtggtg tcgcggagga caccgtggtc
     2041 caccacctcg gccaccagca gctcgttgtc gtgccggagc ccggcgatga gctgcccctt
     2101 ggcgagaagc ttggcgtcct gcaccgcgcc gaagcgctcg agcacctcga ccacccggag
     2161 aaactcttgc cagtacgcgt tgcggaaggc gtcgagcatc tcccgcttct gggcgagctg
     2221 gcgctccacc ccgtcgaggc ccttccaagc ctgctcgcag cggctctgcg agccccacgg
     2281 gcagcggtgg cactcgatgg cggcgagcgc gccctccggg ccgtggctct tctcgcgctc
     2341 gatcagctcg ggtaccgaca ggcgcgccat ctgctcggtg agcccgcggg tgcggtgctc
     2401 ccagtcgcgc ggcacgtgca gcggcggcgt ctgccagaag acgcgcttca cgttgccttc
     2461 cttcatctgc acggtgccgc cgtgggccag cagcacctgg atgagatcgc ggtgccgccg
     2521 ccggtccacg cccacgatga gcccgagggt ggccccgctc ttccgccgca ccagcgcgag
     2581 gcgcccgggc tccgcgtcgg cggcggcacg gccgcgccgc ccgccccgcc cgcgcgccat
     2641 ccgcgccacc tcgacctcgg agcgggcctg ccggtagcgg ccgatgcgcg cgaagtcgcc
     2701 gcagggcgcg tcaaagcgct ggccgtccgc caccgccatc tcgaggctcc gcacgtcggt
     2761 ctcgagcgac ttgatgcgct tcaggttctg gtactggccg aaggaggact cgacggttcg
     2821 ccggatcgtc gccaggtcgt tgccggtcga gagcaggagc gccaccgaac cgtagcccag
     2881 cttgaactgg ctcgcgatcg gctcgggggg gccgttcacc acccgcttca tgtcgtcgag
     2941 cccgtcgcgc acgtcgaggg ccatcacgca cttgccctcg ggatcgatgc cgcggcggcc
     3001 cgcgcggcct gccatctggg tcagctcgtt gtgggtcagg ctgcggaagc cgcggtcggt
     3061 gcgcttggtc agggactgca gcatcacgct cttggccggc atgtggatgc cgagcgacat
     3121 cgtctcggtg gcgaacacca ccttgcagag gccccgctcg aagagcagct cgatgaagcg
     3181 cttcacggtg ggcaggatgc ccgcgtggtg gagccccacc cccatgccga gcgcctggaa
     3241 gatggtctgg ttgagcggcg actccgcgat ggtcgggttc tcggtgacct gctcgcggat
     3301 cgcctcgtcc acctgccgct gctgctcccg cgtgagcagc ggcttgccct cggccaggac
     3361 ggtctccatg gcccgctcgc agccggcacg gctgaagatg aagtagatgg cgggcagcca
     3421 cccgcggctc tccagctcgt cgagcatcac ggtgggatcg accacccggc gcgtgtacca
     3481 gcgcccgcgg tcgttgtagc cgccctcgcg ctcctcgccg agcaggcgca ccttgccctg
     3541 gcggaccgcg tcgtaggtgt ggatctcacc cgagaggtcc gcgaccaggt actgcagcgg
     3601 caccggccgg tgcggatggg tgatggccac gatgggccgg tgcaccaggc tgatccagtc
     3661 cgcgatctcg tccacgttcc gcacggtggc ggacagggcg accagggcca cgtccttcgg
     3721 gcagttgacg atgatctcct cccacaccgt cccccgcccc tcgtcgccca tgtagtggca
     3781 ctcgtcgagc accaccgtat cgaggccctc gagcccgccc gagtagaaca tgttgcgcag
     3841 gatctcggtg gtcatcacca ccaccggcgc gcgggagttg accttgacgt cgccggtgag
     3901 gatgccgacc tgctcggcgc cccactggcg gacgaagtcg gcgtacttct ggttggagag
     3961 cgccttgagc ggggtggtgt aggcgatacg gcggccgcgg tgcagcgcgg catggatggc
     4021 gaactcggcc accagggtct tgcccgagcc ggtgggcgcg gacacgatca ccgacttgcc
     4081 gtcgaggatg gccgcgatgg cccggagctg gaagtcgtcg agcggaaact ggtagcgggc
     4141 cctgaacgac tcgaggagct gctctgccac ggccacggca cctcccttgc ccgcgccctc
     4201 acgttaccac ggccttgcgc ggccgcgaag ccccgagtag gctcggggcc atgaaggtca
     4261 ccggcccatg aagctcaccg ggaaagtcgc cctcatcacc aacgtcagcc acttcatggg
     4321 ccccgccatc accgaggaat tctgtcgcga gggcgcctcg gtagcgctgc acgaccgcga
     4381 cgaggctgcg atcaagccct acgcgctcat cgcggagcgc ctcgggcgcg acgtgctgtc
     4441 gctcgggggc gacctgagcc gctcgggcga ggcggagcgc gcggtgcagg cggtggtggc
     4501 gcgcttcggc cggctcgaca tcctcgtcaa caacaacgcg cacccgccct ccgggagccc
     4561 ggtcgaggag atcaccgacg cggcgtggcg cgagatgatg gcgcggctgc tcgacgagcc
     4621 gttcttttgc ctgcgtgccg cgctccgggt gatgcggccg gccgggcgcg gcaagatcat
     4681 caacatgtcc tcggcggccg ccttccccgg ccttcccaac tacgcggcct acaccgcggc
     4741 gcgcgcgggg gtgaacggcc tcaccaaggc ggtcggccgc gaggtggcgg gcgcgggcat
     4801 ccaggtcaac gcgatcgccc agaactacgt cgagaacccg acctacttcc cgcccgccct
     4861 caccggcgac ccggcccggc tcgcgcgcat ggtcaagaac atcccggcgg gccggctcgc
     4921 ccgcagcgag gagtcggccc ggctggccgt gtacctggcc tccgaggacg ccgacttctt
     4981 catcggccag gtcatcccct tcgcgggcgg ctgggtcacg ccgtgatcgt ctaccacgtg
     5041 gtggtgaaga cgggcgacga ctacctgcag acgcgcgagc cacatcgcaa ggcgcacctg
     5101 gagcggctca tgggactccg ggcgcagggg ctcgtcatcg gcggcggccc caccccggac
     5161 gggcgcggcg cggacctgtt ctaccgcgtc cagcagccag ccgacctgcg ccgcctggtg
     5221 gaagaggacc cctatttcac cggcggggta tggccgggct acacgccgcg ctccttcgcc
     5281 cagttcctcg agccatggga gtcgccgccg ctcgtcaccg acggctctcg ccgggtcacg
     5341 ctggtggaag gactggcgcc cgacgtggag atggcctcct tcgcgctgat cgaggcgcgc
     5401 ggcagtgggc gcatggcctt cggcggcttc ttcccgggcg gcgagacgct cgcggtcatg
     5461 cgcggcgacg acgccgcgaa ggcgctcgac gagctgcgcg ccaccggctt ctgggacgag
     5521 gcctcgctgc gcgcccggcc cctgctgtac gcgctctaac gcgccccctc accctcgcgc
     5581 cccctcaccc tgccctctcc ccctccgggg gcgagggaac tcgacagaac cttctcccct
     5641 ctggggagag aaacttcttc gttgacagcc cccaaactga gtaggtatac ctaatcatca
     5701 tcgtatcagg gccctaagag tgaacggaaa aggcatccga atgatcgagg gtgaggctcc
     5761 gagcgtgcgg gagacaacgc gctgaggcgt cgcctggttc cgatcgccat cctgctcgtc
     5821 ctgaccgcgg cgtccggctt caccgcggcg gcggccgccg ccgatccggt cgccccgatc
     5881 ctccacatgc tcgactacct gggcgtggac tacccggagg cggtgaagga cggcaaggtc
     5941 gccgaccagg gcgagtacga cgagcagctc gagttcgccg cgagcgtgct cgcgacgatc
     6001 ggcgagctgc ccgcccggcc ggagcgcgcc gggctcgagg cggcggccgc gcgcttggcg
     6061 ggcgcgatcc gcgacaagcg gcccggcgcc gaggtcgcgg cgatcgcggg ggagctgcgg
     6121 gggcgcatca tcgaggtcta ccaggtgcgc gtgtcgcccc ggcacgcgcc ggatctggcg
     6181 tccgcccccg cggacttcgc tacccactgc gcggtctgcc acggggccgc cggccggggc
     6241 gacggcccca cggcccgagg gctcacgccg ccgcccgccg atctcaccga cgcggcccgc
     6301 caggcccagc tcagcgtatt cgggctctac aacacgatca cgctcggtat caagggcacc
     6361 gccatgaccg gattcccggc gctcagcgac gcgcagcggt gggggttggc ctttcacgtg
     6421 agcacgctcg cgggcgcagg cgacgagcgc ctgcgcggcg ccgcgctctg gaaggggggc
     6481 accgggcggc gcgagctcgg cgacctgcgc gccctcgtga tggccacgcc ccgggaggtg
     6541 acggcgcgcc tcggcggcga cggcgcggcg gtgctcgcct acctgcgcgg cgagccgggc
     6601 gcggtcgccg ccggcggcga gtccgctctc gatcggagcg cgcgcctgct cgacgagagc
     6661 ctcgaggcct accgcaaggg cgacgcggcg cgcgcgcacc agctggccgt caccggctat
     6721 ctcgaaggct tcgagctcgt cgagaccccg ctcggcgcgg tggacggcgc gctcaagacc
     6781 cgcgtcgagg ccgagatgat gcgctaccgg tccatgctcc aggcgggcgc gccggtggac
     6841 gcggtcgcgg cgcaggcgcg cgccatccag gcgctgctcg tcaccgcgcg ccagcgcctg
     6901 gacgcggccc ggctgtcccc cgccaccatc ttcacgagcg cgctcgtcat cctcctgcgc
     6961 gaggggctgg aggccatcct ggtgctggcc gcgctcgtgg ccctgctgat caagtcgggc
     7021 cgccgcgagg cgctgcgcta cgtgcacgcg ggatggatcg ccgcgctcgc gctcggcggc
     7081 ctgacctggc tggccgcttc gtacctggtg accgtgaccg gcgccgggcg cgagatcacc
     7141 gagggcgtca ccgcgctcct ggccgccgcc atgctgctct acgtgggttt ctggatgcac
     7201 cgccacgccc acgccgcgcg ctggaaggcc ttcatcgaga cgcgcgtggc gtccgccctc
     7261 tcgggcggca cgatgtgggc gctcgcgtcc atcgccttcc tcgcggtcta tcgggaggcg
     7321 ttcgagaccg tgctcttcta ccaggccctc tggctcgagg cgggcccgga cggacgcctc
     7381 gcggtcggcg cagggttcgc cgccgccgtc gtggccctcg tggtgctggc ctggctcatc
     7441 ctgaaggtgg gcctgcggtt gccggtcggc tggttcttcg gcgggggcgc gatcctcatg
     7501 gccgtcctgg cggtggtgct cgccggcaag ggcatcgcgg cgctccagga agccggcacc
     7561 ctgcccatcg ggccgctgga tctgcccgcg atcccgtcgc tggggctcta cccgacctgg
     7621 cagggcgtgc tgacccaggt cgcggtggtg ctgctgatcg tgggggcctt cgcgtattcg
     7681 cggcggcggg aagcctgacc acccctcacc ctaccctctc ccctgagggg agaggggttg
     7741 caggacaccc tct
//
LOCUS       DASUFA010000149        15261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_836549, whole genome shotgun sequence.
ACCESSION   DASUFA010000149 DASUFA010000000
VERSION     DASUFA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 15261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15261
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836549"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFX87_05265"
     CDS             <1..452
                     /locus_tag="VFX87_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity nickel-transport family protein"
                     /protein_id="HEX4994353.1"
                     /translation="HATGPTLHSHAHAHGEAVHRHPHAHPPARLLRALQTVGPAQAIR
                     SALVGLVHGLAGSAAVALLVLSTIRSTAAAVGYLVLFGFGTVLGMTAITGLLSLPFTI
                     RGPRMARGHRALALATGALSLAFGLYMAFEIGFVDGLFLGRAGTIPR"
     gene            complement(479..925)
                     /locus_tag="VFX87_05270"
     CDS             complement(479..925)
                     /locus_tag="VFX87_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008075884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEX4994354.1"
                     /translation="MISAFISSLHLLALAIGLPAVFLRGRALKGGLDADGLRRLLAAD
                     NAWGIAAVLWIVTGLLRAFGGLEKGTEFYLRSPLFWAKVGLFALVLLLEIRPMVTLIR
                     WRMQLGRGQAVDTSPSRALYTINHIELAIVVAMIFVASLMARGIGF"
     gene            complement(965..1891)
                     /locus_tag="VFX87_05275"
     CDS             complement(965..1891)
                     /locus_tag="VFX87_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4994355.1"
                     /translation="MAAKASRAAFGEALIELGAKDERIVTLDADLSKSTMTAGFAKKY
                     PGRAFNLGIAESGMIGVAAGLALTGHVPFACSFACFLVGRFETIRVSVAYSDAPVKLV
                     GTHVGVAIGEDGYTQMGLEDIACLRSLPNIPIIQPADELETKQAVAYAVEHPGPVYLR
                     LTRQNLEPVHDAAYRFQFGKADVLRAGHDVTLLATGGPLWNTLEAAKKLAAEGIQAEV
                     INIATIKPLDEPAILRSAGRTGHVVTVEDHSIHGGLGSAVAELLSEVMPTPMKRIGVT
                     TFGESGDAKGLYAKHGLDPDGIAGGVRKFLNR"
     gene            complement(1891..2787)
                     /locus_tag="VFX87_05280"
     CDS             complement(1891..2787)
                     /locus_tag="VFX87_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX4994356.1"
                     /translation="MANPDLAKLESIAREGRAQIIRMLTHAGSGHPGGSLSVIDILTA
                     LFFNRMRYDPKRPVWDDRDRFVLSKGHCVPAQYYCMARSGYFPMDRLITLRKLGSPLQ
                     GHPDRGALPGIEAATGSLGQGLSVAMGMALGLKLGDKGARVYCVVGDGEIQEGQVWES
                     LMSAPKLGAPDHHLDNLCVILDYNGIQLDNFVKKILDLEPVTDKLKSFGWPVLEVNGH
                     DIAQVDKALDQAEATKGGPTFIVAHTIKGKGVSFMENDPEWHGKSPKPEEAVRAVQEI
                     VGSPVPVWAEPIIAELSALGKK"
     gene            complement(2787..4337)
                     /locus_tag="VFX87_05285"
     CDS             complement(2787..4337)
                     /locus_tag="VFX87_05285"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX4994357.1"
                     /translation="MDLTRAVQEALACLARQPGVLEIEVFAAFNRSLLTRLNYTSHIP
                     CNGVEEPKSTEMAGLGVQAVFDGPDGRPLIGFGSEPSDLTPDGALRALAKARQAAVHD
                     PEFRSLPRPIGTPRALAGHHDPRLMNVQDAELVEAGWKVITGGLRAFMASSRLADLVA
                     GETDLKRLGLILGGDVSILQSRVAIASSAMPEPQADDCAFMSASATGMVEARDAKGSG
                     WSITTRLEHFTDEAGVEAAGNAVAAMDGERVLSGDYTVIFGPQPVADLCSNLIVPSCQ
                     AGSFYSSSTPFLGRLGRVVAARGLSIYDHGAMPGLAGSRGITCEGLPTGRTDLIKDGV
                     LVGLLSHWYDTQRLLHDPAARAKLGVDPAAAAAALAPRNGFRGDSGSGRSFESTPGTS
                     ASNVIVEGADPVSLADLAARVGDGLYVGRIWYTYPINGLRAGDFTCTVVGDSFIIRDG
                     RIAEPLRANTIRINDNVTRLLGAVVGTTKDARGTPLWAADEVVYAPAIAVSGVHVDAI
                     AGFMEGLG"
     gene            complement(4342..5910)
                     /locus_tag="VFX87_05290"
     CDS             complement(4342..5910)
                     /locus_tag="VFX87_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX4994358.1"
                     /translation="MPHGIETRLIETVTPMVRELVTTQARALADLHYADVRLEVSEGK
                     FAGAENGEAKTSGDDYAFALGIRVLAGRRMTAPGYFGRGLGAADVPHLARLLREGLLT
                     AYRRAMANAEMKAEVKGKFGALGDALADTRLHPIAVRQAVVPAVFEVDPRSIALDDMQ
                     RLTTDVSRRVAGFHASASYNYISTMTQLSRELFASSEGAVIDQSFALTQGMCYVVATG
                     ADNSQEIYDVLGHQRGWEILTRGVSEPLIEFPPFPEFALALAGDAVELAAAPALAAPD
                     GEVAVVTDPHYNTLLSHEIVGHPVELDRGLKMETAYAGRSWLLRGLDDTQLGKRIASP
                     LVSAFSDPSLPGYGHYAYDHEGTPARRVVHIDRGVFTGFMNSRQTAAVFGGDPNGHWK
                     ATDASLVPLVRMSSTVFAGGDREPTDLIKEIDHGYFLVGHRTPSIAESRENFRITARK
                     VYEIHRGQIGRLYRAGGIMSDSRDFLMHVDGVGRDFRLYPIPNCGKGQPMQTRKLGNG
                     GPTLRSRAIVTGGA"
     gene            complement(5915..7117)
                     /gene="glp"
                     /locus_tag="VFX87_05295"
     CDS             complement(5915..7117)
                     /gene="glp"
                     /locus_tag="VFX87_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX4994359.1"
                     /translation="MISVADAQAHILAQVTRIAPPEVAAVGACLGRVLAEDVRATFDV
                     PPTDNSAVDGYAVRSEDVPPDGSRELTVVAELAAGAVFDGTLGPGEALRIMTGAPMPA
                     GGGTVYPQEIVERSGRSVRVPAGPAGVNVRHRGEDVRAGSLVLAAGTALRPQELGVAA
                     SLGLPQLLVHQRPRVAILSTGDEVAEPGDTRKPGQIFDSNRFSLHGLVHAAGGQTTDH
                     GIVPDLYDVLHARLRTAAATADVVITSGGVSVGEHDLVKAVLQDAGGIDFWQVAMQPG
                     RPLAVGRIGSAHFFGLPGNPVASMLTFHLFVRPALWKLAGRRELFPPRFHAVAMEPMG
                     KKPGRREFKRGILSYSGERWEVRITGPQGSGILTSMTLANCFVVIEEERGDVAAGDTV
                     SVEPFSLA"
     gene            7261..7815
                     /locus_tag="VFX87_05300"
     CDS             7261..7815
                     /locus_tag="VFX87_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4994360.1"
                     /translation="MDVARYRADLAAVSATPAPAVEPATPPAAAMPAPGPITTAAVRV
                     FAPGPTGALHPAGFWIRAGALLIDVVLVTVAEAAFGFLLWAVADDRLAAAASRAFRFL
                     ASPCYFVLLHWARGQTLGKMAFRVRVVSLHGGPLSFGQAALRHLGSWLSAVILGIGYL
                     IAAFRADKRALHDLIASTRVEHVT"
     gene            7827..8351
                     /locus_tag="VFX87_05305"
     CDS             7827..8351
                     /locus_tag="VFX87_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4994361.1"
                     /translation="MATCPMCRAPHAHLGSTRPLAALRQAPARLARALRRVPRRLVAR
                     RPGRGEWAGAEVLGHLADAEIALAFRIRKIAAEPRGVLAAWDQDRWADHGGYRRTPPG
                     EALATFAALRRSNLAYVGRLSAAQMGQYGRHPEYGRLTIPQMLAHWAEHDLNHLAQIR
                     AAHRRLAGRRSGRP"
     gene            8348..8998
                     /locus_tag="VFX87_05310"
     CDS             8348..8998
                     /locus_tag="VFX87_05310"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX4994362.1"
                     /translation="MTLPLPLRNRLADLILDALHDGAARRGLEAVAAVCADPAALDPA
                     APPPERFPSELFDRRADGWRIRSGFGPHAAAFGERAARAARALAGRPLAAADPPLPVG
                     LAEAATLFEAGLYFETHEVLEPFWMRAEGARREALQGLIQVAVGFQHLANGNLEGARA
                     LLRDGATKLAGHRLDGRDTSTFAAEVARCLGEILALGAEASARFDWSRVPRFPAAD"
     gene            9005..9550
                     /locus_tag="VFX87_05315"
     CDS             9005..9550
                     /locus_tag="VFX87_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994363.1"
                     /translation="MARVHALSDATEVARRRKLLPPGRLLEAWPDLYTPGRAWVGDEA
                     RALLETAGGALPIALSVDATAVPVYYGPRLSDVESLPLEESLRARVLSARGIAVAWIT
                     YDQFGERTVYEPTGPADPIFYLRRPAGTAVHIWRLFRTKQEARVYMAEYYGRDSEGRD
                     WAETLPEETFDDLLARQRPRG"
     gene            9576..10370
                     /locus_tag="VFX87_05320"
     CDS             9576..10370
                     /locus_tag="VFX87_05320"
                     /inference="COORDINATES: protein motif:HMM:NF004779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX4994364.1"
                     /translation="MDLGLKGKTALVTGGSKGIGRAVAQSLAAEGARVMICSRDAGTL
                     KQAAADIERATGGHVETTSADLSRHAEVVRVAKDAVTRLGRLDILVNNAGAIKGGDFL
                     ATPDEEWLDGWSLKLLGYIRMAREVLPHMQRQGGGRIVNVVGAAARNPAVTYMMGGTA
                     NAALINFTKALSDLAVKSKVLVTGVSPGPVKTERWDSLMAQQAKAAGQDADDYVRERS
                     QEFPLGRIALPEEVADVVCFLASDRASYLTGITITVDGGITRGVYL"
     gene            10531..10926
                     /locus_tag="VFX87_05325"
     CDS             10531..10926
                     /locus_tag="VFX87_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX4994365.1"
                     /translation="MTPLASREGVYSVLIAAGGSAMPAKLFVGNLSFQATEEDLRELF
                     QQAGTVESVRIVTDQFTGRPRGFGFVEMATKEELAKAIEMLNGRLFRDRNLVVDEARP
                     QPQRTGGGGGPRGGGGGRPGGGGGGGWRR"
     gene            11008..11985
                     /locus_tag="VFX87_05330"
     CDS             11008..11985
                     /locus_tag="VFX87_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4994366.1"
                     /translation="MDRRPAPGFVKRLGGLVDHRRGVTFRALKSENLLNRLTGAGMPF
                     GWTVNPFRGCELGCRYCYARPTHEYLGHGDPDEFEERIYVKEAGTARLLSGLIRARDA
                     GQEIAIGTATDPYQPAEARFAVTRGVLEVMARVPGLRIGLTTKSTGVLRDRELLARIA
                     RASDLWVNISLISLDAPLLRQLEPRAPRPDLRLEAMRALSAEGVRARLFLMPVLPLLT
                     DGDAGLRELLAAALAAGARETISQALFLRTEMTWRFFLDFVEREFPWALARYRALYPA
                     PGNAPRAYREEIERRVAQRAAEVGFPARTRDDRVRAEAPARPRQLSLVW"
     gene            complement(11999..14287)
                     /locus_tag="VFX87_05335"
     CDS             complement(11999..14287)
                     /locus_tag="VFX87_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX4994367.1"
                     /translation="MTSIPLPFYDNPLLFGHDPARGLLAFRPAESHVTVYALRSGLVI
                     TADEPFRPFLLLADPDLLKELKGDVEVTPLDGDGVYRWLAECPSWPQALRVRDHCLRV
                     SGKSAGAPDAPYRFLGDPVHQFLLRSGKTSFLGMGFGDLRRMALDIEVTTAPGFEFPN
                     AARESDRIIAIAMADSTGFSTVLSGAELSEAQLLAECSRILRERDPDVLEGHNIFRFD
                     LEYLEARARRHRVPLAWGRDGSVLRGYPSRMQVAERAIGYRRYGVTGRHIVDTWILAQ
                     LYDVGARDLESYGLKDVARHFGVPAADRTYLPPEDIPRIFVEDPARLMAYARDDVLET
                     LAISAILSPPYFVQAQALPFDYQTTVLRGNATKIDALLMREYLHRRQAVPAPGQGRGV
                     AGGYTAVFQQGVARDVLHVDVTSLYPSLMLGQDLFPASDRLGVSASLLRDLREFRVSA
                     KRLAREAAAPEDRIFLNALQQTFKILINSFYGYLAFSQGHWNDFDVANRVTGEGRRLV
                     QALLDRLGELGATVVEVDTDGIYFVPPAASPAVSEGHLVSELSAVLPEGIQLELDGRY
                     VAMFSYKMKNYVVLDERGKLLIKGSGLRSRGIELFQRLWMEEMFRLLLTGRREEIPAL
                     VSRWEEDFRAHRVPLKQFMKTETLQESLAGYRQKLEAGARNVGAAYELAQGSSRSYQP
                     GDQVSYYVTGDSKRVKVNEAAKLAAEWSPSAPDENTAYYVSKLQELYEKFRPLIEQDG
                     LSPVVPTEPEPAAPEQGRLYED"
     gene            complement(14284..14778)
                     /locus_tag="VFX87_05340"
     CDS             complement(14284..14778)
                     /locus_tag="VFX87_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994368.1"
                     /translation="MSDEPARETRAEAHRHFQQGYEAQMQGRLEEAVAHYQRSIALHP
                     TAEAHTFLGWAYSHQGRPDDAIAQCKIAISVDPDFGNPYNDIGAYLIELGREEEAVTW
                     LERAKKAVRYEPRHYAYFNLARSHIKRHEIFAAIRELEGALAIEPRYVTARKELHRLR
                     GLLN"
     gene            14885..>15261
                     /locus_tag="VFX87_05345"
     CDS             14885..>15261
                     /locus_tag="VFX87_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4994369.1"
                     /translation="MYRLAAAALATTLLTGCAAHTARQTVHFPNSTPGAPVTIAATLV
                     RPAGPGPFPAVVQLHGCAGLEAQSFRWARGLAERGYVALVVDSFGPRGVKSDCRSGPD
                     DPPITARLDDAFGALRYLQSQTYV"
BASE COUNT         2122 a         5486 c         5454 g         2199 t
ORIGIN      
        1 cgcacgcgac gggccccacg ctgcacagcc acgcccacgc ccacggggag gcggtgcacc
       61 gccatcccca cgcgcacccg cccgcgcggc tgctccgcgc gcttcagacg gtgggcccgg
      121 cccaggcgat ccgctccgcc ctcgtggggc tcgtgcacgg cctggccggg agcgcggcgg
      181 tggcgctgct cgtgctctcc acgatccgca gcacggcagc ggcggtgggc tacctcgtgc
      241 tgttcggctt cgggacggtc ctgggcatga ccgcgatcac cggcctgctg tccctgccgt
      301 tcacgatccg cggcccccgc atggcgcggg gacaccgggc gctcgccctc gccaccggcg
      361 cgctgtcgct ggccttcggc ctctacatgg ccttcgagat cggcttcgtg gacggcctgt
      421 tcctcggccg cgcggggacg atcccgcgct agccgggatc acgccccggc acccgcggct
      481 agaagccgat cccgcgggcc atcaggctcg ccacgaatat catcgcgacc acgatggcca
      541 gctcgatgtg gttgatcgtg tagagcgcgc gggacgggct cgtgtccacc gcctggcccc
      601 ggccgagctg cattcgccag cggatgaggg tgaccatcgg cctgatctcg agcagcagca
      661 cgagcgcgaa cagccccacc ttggcccaga agagcggcga ccgcaggtag aactccgtgc
      721 ccttctcgag gccgccgaac gcgcgcagca ggccggtgac gatccacagc acggccgcga
      781 ttccccaggc gttgtccgcg gccaggagcc ggcgcaggcc gtcggcgtcg agccccccct
      841 tgagcgcgcg gccgcgcagg aacaccgcgg gcaggccgat ggccagcgcg agcaggtgga
      901 gcgaggagat gaacgcggag atcatgcgcg gaacctggcg cgcgctcgga ggccgcgcgc
      961 cgggctagcg gttgaggaac ttccgcacgc cgcccgcgat cccgtccggg tccagaccgt
     1021 gcttggcgta gaggcccttg gcgtcgccgg actccccgaa ggtggtcacg ccaatccgct
     1081 tcatcggggt cggcatcacc tcggacagga gctccgccac cgcgctgccg aggccgccgt
     1141 gaatggagtg gtcctccacc gtgaccacgt gcccggtgcg ccccgcggag cggaggatgg
     1201 ccggctcgtc gagcggcttg atggtggcga tgttgatgac ctcggcctgg atcccctcgg
     1261 ccgcgagctt cttcgcggcc tcgagcgtgt tccagagcgg gccgccggtg gccagcagcg
     1321 tcacgtcgtg ccccgcgcgc agcacgtcgg ccttgccgaa ctggaagcgg taggccgcgt
     1381 cgtgcaccgg ctcgaggttc tgccgggtga gccgcaggta gaccggcccc gggtgctcga
     1441 ccgcgtaggc gaccgcctgc ttcgtctcga gctcgtcggc gggctggatg atcgggatgt
     1501 tcggcagcga gcgcaggcag gcgatgtcct cgagccccat ctgcgtgtag ccgtcctcgc
     1561 cgatggccac gcccacgtgg gtgccgacca gcttgacggg cgcgtcgctg taggcgaccg
     1621 agacccggat cgtctcgaac cggccgacca ggaagcaggc gaaggagcac gcgaagggca
     1681 cgtggccggt gagcgccagt cccgcggcca cgccgatcat gccggactcg gcgatgccga
     1741 ggttgaaggc gcgccccggg tacttcttcg cgaagccggc ggtcatggtg gacttggaga
     1801 gatcggcgtc gagcgtcacg attctctcat ccttggcgcc cagctcgatc agcgcctccc
     1861 cgaacgcggc gcgcgaggcc ttggccgcca tcacttcttc cccagggcgg acagctccgc
     1921 gatgatcggc tcggcccaca ccggcaccgg gctccccacg atctcctgga ccgcccgcac
     1981 cgcttcctcg ggcttgggcg acttgccgtg ccactccggg tcgttctcca tgaaggacac
     2041 acccttcccc ttgatcgtgt gcgccacgat gaacgtgggc ccgcccttcg tggcctccgc
     2101 ctggtccagc gccttgtcca cctgggcaat gtcgtggccg ttgacctcga gcaccggcca
     2161 gccgaacgac ttgagcttgt cggtcaccgg ctcgagatcg aggatcttct tcacgaagtt
     2221 gtcgagctgg atgccgttgt agtcgaggat cacgcagaga ttgtccaggt ggtgatcggg
     2281 cgcgccgagc ttgggtgcgg acatgaggct ctcccagacc tggccttcct ggatctcgcc
     2341 gtcgcccacc acgcagtaga cgcgcgcgcc cttgtccccg agcttgagtc cgagcgccat
     2401 gcccatcgcg accgacaggc cctggccgag cgagccggtc gcggcctcga tgccggggag
     2461 cgccccgcgg tcgggatgtc cctgcagcgg gctgccgagc ttgcgcaggg tgatcaggcg
     2521 gtccatcggg aagtacccgg accgggccat gcagtagtac tgggcgggca cgcagtggcc
     2581 cttcgagagc acgaaccggt cgcggtcgtc ccacacggga cgcttggggt cgtagcgcat
     2641 gcggttgaag aagagggcgg tcaggatatc gatcacggag agcgagcccc cgggatgccc
     2701 cgaccccgcg tgggtgagca tccggatgat ctgcgcccgc ccctcgcggg cgatggattc
     2761 gagcttcgcc aggtccggat tcgccatcag cccagtccct ccatgaagcc tgcgatcgca
     2821 tccacgtgca cgccgctcac cgcgatcgcg ggggcgtaca cgacctcgtc ggctgcccac
     2881 agcggggtgc cgcgcgcgtc cttggtggtg ccgacgacgg ctcccagcag ccgcgtcacg
     2941 ttgtcgttga tccggatcgt gttcgcccgg agcggctccg cgatccggcc gtcgcggatg
     3001 ataaaggaat cccccaccac cgtacaagtg aagtcaccgg cccgcaaccc gttgatcggg
     3061 tacgtgtacc agatgcgacc cacgtagagg ccgtcgccga cgcgcgcggc gagatccgcg
     3121 agcgacaccg gatcggcgcc ctcgacgatg acgttggacg ccgacgtgcc cggcgtgctc
     3181 tcgaaggacc ggccggagcc gctgtccccc cggaagccgt tccgcggcgc gagcgcggcc
     3241 gccgcggcgg ccggatccac cccgagcttc gcgcgcgcgg ccggatcgtg gagcagccgc
     3301 tgcgtgtcgt accagtggct gagcagcccg accagcacgc cgtccttgat caggtcggtc
     3361 cggccggtgg gcagcccctc gcaggtgatg ccgcgcgagc cggccagccc cggcatggcc
     3421 ccgtggtcgt agatgctgag cccgcgcgcc gcgacgacgc ggcctagccg ccccaggaac
     3481 ggggtgctcg aagaatagaa cgagcccgcc tggcaggagg ggacgatcag gttgctgcag
     3541 aggtcggcca ccggttgcgg accgaagatc accgtgtagt cgcccgagag cacccgctcg
     3601 ccgtccatcg cggccaccgc gttgcccgcc gcttccacgc cggcctcgtc ggtgaagtgc
     3661 tcgagccggg tggtgatgga ccacccggag cccttggcgt cgcgcgcctc caccatgccg
     3721 gtggccgacg ccgacatgaa cgcgcagtcg tcggcctggg gctcgggcat cgcgctggag
     3781 gcgatcgcca cccgcgactg gagaatgctc acgtccccgc ccaggatgag cccgagccgc
     3841 ttgaggtccg tctcgccggc gaccaggtcg gcgagtcgag acgacgccat gaaggcgcgc
     3901 agcccgccgg tgatcacctt ccagcccgcc tccaccagct cggcgtcctg cacattcatg
     3961 agccgcggat cgtgatggcc cgcgagagcc cgcggcgtgc cgatgggtcg cggcagcgag
     4021 cggaactccg gatcgtgcac cgcggcctgc cgcgccttcg cgagcgcccg cagggcgccg
     4081 tccggagtga gatcgctcgg ctccgagccg aagccgatca gcggccgccc gtcgggaccg
     4141 tcgaacaccg cctggacgcc gagccccgcc atctcggtgg acttcggctc ctccacgccg
     4201 ttgcacggga tgtgcgaggt gtagttgaga cgcgtgagca gggagcggtt gaacgcggcg
     4261 aagacctcga tttccagaac gccgggctga cgcgcgaggc aggcgagcgc ctcctggacc
     4321 gcccgcgtca gatccacgcg gtcaggctcc gccggtcacg atggcgcggc tgcgcagggt
     4381 cgggccgccg ttgccgagct tgcgcgtctg catgggctgg cccttgccac agttcggaat
     4441 cgggtacagc cggaagtcgc ggcccacgcc gtccacgtgc atcaggaagt cgcggctgtc
     4501 cgacatgatg ccgcccgccc ggtagagccg gccgatctgg ccccggtgga tctcgtagac
     4561 cttccgggcg gtgatgcgga agttctcgcg actctccgcg atggagggcg tccggtggcc
     4621 gacgaggaag tagccgtgat cgatctcctt gatgagatcc gtcggctccc ggtctccgcc
     4681 cgcgaacacg gtggaggaca tgcggacgag cgggaccagc gaggcgtcgg tggccttcca
     4741 gtgaccgttc ggatcgccgc cgaagaccgc cgcggtctgc cggctgttca tgaacccggt
     4801 gaacaccccg cgatcgatgt ggacgacccg gcgcgccggc gtgccttcgt gatcgtaggc
     4861 gtagtgcccg tagccgggca gggacggatc ggagaacgcg ctcaccagcg gggaggcgat
     4921 gcgcttgccg agctgggtgt cgtcgaggcc gcgtagcagc caggaacgcc cggcgtacgc
     4981 ggtctccatc ttgagcccgc ggtccagctc gaccgggtgc cccacgatct cgtgggagag
     5041 cagggtgttg tagtgcgggt cggtgacgac cgccacttcc ccgtcgggcg ccgcgagcgc
     5101 gggcgcggcg gccagctcca ccgcgtcccc ggccagcgcc agtgcgaact ccgggaaggg
     5161 cgggaactcg atcagcggct cggagacacc gcgtgtgagg atctcccagc cgcgctggtg
     5221 gccgagcacg tcgtagatct cctggctgtt gtcggcgccg gtcgccacca cgtagcacat
     5281 gccctgggtg agcgcgaagg actggtcgat gaccgcgccc tccgaggagg cgaagagctc
     5341 gcggctcagc tgggtcatcg tggagatgta gttgtaggac gcgctcgcgt ggaagccggc
     5401 cacccgccgg gacacgtcgg tggtgaggcg ctgcatgtcg tccagcgcga tcgatcgagg
     5461 gtcgacctcg aagaccgccg ggaccaccgc ctggcgtacc gcgatggggt gcaggcgggt
     5521 gtcggcgagc gcatcgccga gggcgccgaa cttgcccttc acctccgcct tcatctccgc
     5581 gttggccatc gcgcgccggt aggcggtcag caagccctcg cgcagcagcc gggccaggtg
     5641 ggggacgtcc gcggcgccga gcccgcggcc gaagtacccg ggcgcagtca tgcggcgccc
     5701 cgcgaggacg cggatcccga gggcgaaggc gtagtcgtcg ccggaggtct tggcctcgcc
     5761 gttctccgcg cccgcgaact tgccctcgct cacctcgaga cgcacgtcgg cgtagtggag
     5821 gtccgcgagc gcccgggcct gggtggtgac cagctcgcgc accatcggcg tgacggtctc
     5881 gatcagccgg gtctcgatcc cgtggggcat gatctcaggc cagggagaac ggctcgaccg
     5941 agacggtatc gcccgccgcc acgtcgccgc gctcctcctc gatcaccacg aagcagttgg
     6001 ccagcgtcat cgaggtgagg atgccggagc cctggggccc ggtgatgcgc acctcccagc
     6061 gctcgccgct gtaggagaga atgccgcgct tgaactcccg ccggcccggc ttcttgccca
     6121 tcggctccat cgccaccgcg tggaagcggg gcgggaacag ctcgcggcgc ccggcgagct
     6181 tccagagggc gggccgcacg aagaggtgaa acgtcagcat cgaggccacc ggattgccgg
     6241 gcaggccgaa gaagtgcgcc gatccgatcc ggcccaccgc gagcggacgg ccgggctgca
     6301 tcgcgacctg ccagaagtcg atgccgcccg cgtcctggag caccgccttc accaggtcgt
     6361 gctcgcccac cgacacgccc cccgaggtga tcacgacgtc cgcggtggcg gcggcggtgc
     6421 gcaggcgcgc gtggagcacg tcgtagagat cggggacgat gccgtgatcg gtggtctgcc
     6481 cgcccgcggc gtggacgagg ccgtgcagcg agaagcggtt cgaatcgaag atctggccgg
     6541 gcttgcgcgt gtcgcccggc tcggcgactt cgtcgccggt ggagaggatc gccacgcgcg
     6601 ggcgctggtg cacgaggagc tgcggcagcc cgagcgaggc ggcgacgccg agctcctgcg
     6661 gccgcagggc ggtgcccgcg gccagcacca gcgagccggc ccgcacgtcc tcgccgcggt
     6721 ggcgcacgtt gacgccggcg gggccggccg gaacccgcac ggagcgcccg ctgcgctcga
     6781 cgatctcctg cgggtacacg gtacctccac ccgcgggcat cggcgcgccg gtcatgatgc
     6841 gcagcgcctc gccgggcccg agcgtgccgt cgaagactgc gccggcggcc agctcggcca
     6901 ccaccgtgag ctcccggctg ccgtcggggg ggacgtcctc cgagcggacc gcgtagccgt
     6961 ccaccgcgga gttgtcggtg ggaggcacgt cgaaggtggc gcgcacgtcc tcggcgagca
     7021 cgcgcccgag acaggccccc accgcggcca cctcgggcgg cgcgatccgg gtcacctgcg
     7081 cgaggatgtg cgcctgcgcg tccgcgacgg aaatcatgac gcgcattata ccgtgcggcc
     7141 cgggcggccg cccgcatccc ccgtcgtacg atgcgcgtgc tacgatacgc gccgatgtcg
     7201 tcgtcatgcc ctcaatgcgg ctggtcgcgg gtcgacggcg cgcactgtcc gcgctgcggc
     7261 gtggacgtgg cccgctaccg cgccgacctg gccgcggtga gcgcgacacc cgcgccggcg
     7321 gtcgagccgg ccacaccgcc cgccgctgcg atgcccgcgc ccgggccgat cacgacggcg
     7381 gcggtgcgcg tcttcgcgcc ggggcccacc ggcgccctcc accccgccgg cttctggatc
     7441 cgcgccgggg ccttgctgat cgacgtggtc ctggtgacgg tggcggaggc ggccttcggg
     7501 ttcctcctgt gggcggtcgc cgacgaccgg ctggccgccg cggcctcgcg cgccttccgc
     7561 ttcctggcca gcccctgcta cttcgtgctc ctgcactggg cgcgcggcca gaccctcggc
     7621 aagatggcct tccgcgtccg cgtcgtctcg ctccacgggg gcccgctctc gttcgggcag
     7681 gccgcgctcc ggcatctcgg ctcctggctc tccgcggtca tcctcggcat cggctacctg
     7741 atcgccgcct tccgcgccga caagcgcgcg ctgcacgacc tcatcgcgtc cacccgcgtc
     7801 gaacacgtca cctgacggag gctcccttgg cgacctgccc gatgtgccgc gccccgcacg
     7861 cccacctcgg ctccacccgc cccctggccg cgctgcgaca ggcgcccgcc cggctcgccc
     7921 gcgcgctgcg tcgcgtgccg cggcggctgg tggcgcgccg gcccgggcgc ggggagtggg
     7981 cgggcgccga ggtcctgggc catctggccg acgcggagat cgcgctcgcg ttccggatcc
     8041 gcaagatcgc ggcggagccg cggggcgtgc tggcggcgtg ggaccaggac cgctgggcgg
     8101 accacggcgg ctaccggcgc acgccgccgg gcgaggcgct cgccaccttc gcggcgctcc
     8161 gtcgctcgaa cctcgcctac gtcggccggc tgagcgcggc gcagatgggg cagtacgggc
     8221 ggcacccgga gtacggccgt ctcacgatcc cgcagatgct cgcccactgg gccgagcacg
     8281 acctgaacca cctggcgcag atccgcgcgg cgcatcgccg gctcgcgggc cggcgctccg
     8341 gcagaccgtg accctgccgc tgcccttgcg caaccggctc gccgatctca ttctcgacgc
     8401 gctccacgac ggcgccgcgc gccgcgggct cgaggcggtc gccgcggtgt gcgccgaccc
     8461 ggccgcgctc gaccccgccg cgccgccccc cgagcgattt ccctccgagc tcttcgatcg
     8521 gcgcgccgac ggctggcgca tccgctcggg cttcggcccc cacgccgccg ccttcggcga
     8581 gcgcgcggcc cgcgccgcgc gcgccctcgc gggccgaccc ctcgccgccg ccgacccgcc
     8641 gctgccagtg ggcctcgcgg aagcggccac gctcttcgag gccggcctct acttcgagac
     8701 ccacgaggtg ctcgagccct tctggatgcg cgcggaaggc gcgcggcgcg aggcgctcca
     8761 gggcctcatc caggtcgcgg tgggcttcca gcatctggcc aacggaaacc tcgagggcgc
     8821 gcgcgcgctg ctgcgcgacg gcgcgaccaa gctcgccggt caccggctgg acggccggga
     8881 cacgagcacc ttcgcggccg aggtggcgcg gtgcctcggc gagatcctcg cgctcggcgc
     8941 ggaggcgtcc gcgcgcttcg actggagccg ggtgccccgc ttcccggcgg cggactgagc
     9001 tgggatggca cgcgtgcacg cgctgtccga tgcgaccgag gtcgcgaggc ggcgcaagct
     9061 gctgcccccg ggccggctcc tcgaggcctg gcccgatctc tacacccccg gccgggcgtg
     9121 ggtgggggat gaggcccgcg cgctgctgga gaccgcgggc ggcgcgctgc ccatcgcgct
     9181 ctcggtggac gcgacggcgg tgcccgtcta ctacggcccg cgcctttcgg acgtggagtc
     9241 gctgccgctc gaggagtcgc tacgcgcgcg ggtgctgtcg gcgcgcggca tcgcggtggc
     9301 ctggatcacc tacgatcagt tcggcgagcg caccgtgtac gagccgaccg gtcccgcgga
     9361 tcccatcttc tacctgcggc ggcccgccgg caccgccgtg cacatctggc gcctgttccg
     9421 gacgaagcag gaggcgcgcg tctacatggc cgagtactac ggccgcgact ccgaggggcg
     9481 ggactgggcg gagaccttgc ccgaggagac gttcgacgat ctgctcgccc gccagagacc
     9541 acggggctga tcgctcaccg ataaggagtc acacgatgga tctagggctc aagggcaaga
     9601 ccgcactggt caccggtggc agcaagggca tcggccgcgc ggtcgcgcaa tcgctcgccg
     9661 cggagggcgc gcgcgtgatg atctgctcgc gcgacgccgg caccctgaag caggccgccg
     9721 cggacatcga gcgcgcgacg ggcggccacg tggagaccac ctccgcggac ctgagccggc
     9781 acgccgaggt cgtgcgggtc gcgaaggacg cggtgacgcg gctcggccgc ctcgacatcc
     9841 tcgtgaacaa cgcgggcgcg atcaagggcg gcgattttct cgcgacgccg gacgaggagt
     9901 ggctcgacgg gtggagcctc aagctcctcg gctacatccg catggcgcgg gaggtgctcc
     9961 ctcacatgca gcgtcagggt ggcgggcgca tcgtgaacgt ggtgggcgcc gccgcgcgca
    10021 accccgccgt cacctacatg atgggcggca ccgccaacgc cgcgctgatc aacttcacca
    10081 aggcgctctc cgacctggcc gtgaagtcga aggtgctcgt caccggcgtc tcgccggggc
    10141 ccgtgaagac cgagcgctgg gacagcctga tggcgcagca ggcgaaggcc gcgggtcagg
    10201 acgcggacga ctacgtaaga gaacgtagtc aggaatttcc actcgggcgc atcgcgctgc
    10261 cggaagaagt cgccgacgtc gtgtgcttcc tcgcgtccga ccgcgcctcg tacctcacgg
    10321 gaattaccat cacggtggat ggcggcatca cgcgcggcgt gtatctctga gcaatttttg
    10381 ttgcccaggc cgcacactca gtaagggcat aacgtagtga aataaagaac attctgccgg
    10441 aagattcgct tgacaggtgg taggaccaac agtagcgtag cgcccacgct gcgggtccgc
    10501 ctggcgaggc ccgcgagcgg atggctttcc ttgacgccgc tggcgagccg ggagggagtc
    10561 tactcggtcc tgatcgcggc aggaggttcc gcaatgcctg caaagctttt cgtcggcaat
    10621 ctctccttcc aggcgacaga agaagacctg cgcgagctct tccagcaggc cgggacggtc
    10681 gaatccgtcc gcattgtcac cgatcagttc accggcaggc cccgcggctt cggcttcgtc
    10741 gagatggcga ccaaggaaga gctcgccaag gcgatcgaga tgctaaacgg gcggcttttc
    10801 agggatcgta acctcgtcgt cgacgaggcc cggccccagc cccagcgtac cggcggcggt
    10861 ggcgggcccc ggggcggcgg cggtgggcgg cccggcggag gcggcggagg cggctggcgc
    10921 cggtaggcac cttcagcccc ggggaagcca cgtagggttc cccggggttt tttctcgtgg
    10981 caaattactc gtcatctgga gagcctcatg gatagacgcc cggctcccgg cttcgtgaag
    11041 cgcctcggcg ggctcgtaga tcaccgtagg ggcgtgacgt tccgggcgct caagtcagag
    11101 aatctgctga atcgcctcac cggggcgggc atgcccttcg gctggaccgt gaacccgttc
    11161 cgcgggtgcg agctgggctg ccgctactgc tacgcccgac ccacgcacga gtacctgggg
    11221 cacggcgacc ccgacgagtt cgaggagcgc atctacgtca aggaggctgg caccgcacgg
    11281 ctgctgagcg gcctgatccg cgcgcgcgac gccggccagg agatcgccat cggcaccgcg
    11341 accgacccct accagccggc ggaagcgcgc ttcgcggtga cccggggcgt gctcgaggtg
    11401 atggcgcgcg tgcccggcct ccgcatcggg ctcaccacca agtccaccgg cgtcctgcgc
    11461 gatcgcgagc tcctggcccg catcgcgcgc gcctcggacc tctgggtcaa catctcgctg
    11521 atctcgctcg acgcgccgct cctccggcag ctcgagcccc gggcgccccg gcccgatctg
    11581 aggctcgagg ccatgcgggc gctctccgcc gagggcgtgc gcgcacgcct gttcctcatg
    11641 ccggtgctgc cgctgctgac ggacggcgac gcgggcctgc gcgagctgct ggccgccgcc
    11701 ctcgcggcgg gcgcgcgcga gacgatctcg caggcgctct tcctgcggac cgagatgacg
    11761 tggcgcttct tcctcgactt cgtggagcgg gagttcccgt gggcgctcgc gcgctaccgc
    11821 gcgctctatc cggcgcccgg caatgccccg cgcgcctacc gcgaggagat cgagcgccgc
    11881 gtggcgcagc gggccgcgga ggtcggcttc ccggcccgca cccgcgacga tcgcgtgcgc
    11941 gccgaggcgc ccgcgcggcc gcgccagctc tcgctggtct ggtgagggca gccccgcctc
    12001 aatcctcgta caggcgcccc tgctcggggg ccgcgggctc gggctcggtg gggacgaccg
    12061 gcgagaggcc gtcctgctcg atgagcggcc ggaacttctc gtagagctcc tgcagcttgc
    12121 tcacgtagta cgcggtgttc tcgtcgggcg cgctcgggct ccactcggcc gcgagcttgg
    12181 ccgcctcgtt gaccttgacc cgcttggagt cgcccgtcac atagtaggag acttgatcgc
    12241 ccggctggta cgaccggctc gagccctggg ccagctcgta ggccgcgccc acgttgcgcg
    12301 cgcccgcctc gagcttctgg cgataccccg cgagcgactc ctgcagcgtc tccgtcttca
    12361 tgaactgctt gagcggcacg cggtgagccc gaaagtcctc ttcccagcgg ctcaccaggg
    12421 cggggatctc ctcccggcgc ccggtgagga gcaggcggaa catctcctcc atccacagcc
    12481 gctggaagag ctcgatgccg cgcgagcgca ggccggagcc cttgatcagg agcttgccgc
    12541 gctcgtcgag gacgacgtag ttcttcatct tgtagctgaa catcgccacg tagcggccgt
    12601 ccagctcgag ctggattccc tccgggagca cggccgacag ctcgctcacg agatggcctt
    12661 ccgacacagc gggggacgcc gcgggcggga cgaagtagat gccgtcggtg tccacctcga
    12721 cgacggtggc gcccagctcg ccgagccggt cgagcagcgc ctgcacgagg cgccgcccct
    12781 cgccggtcac gcggttggcc acgtcgaagt cgttccagtg cccctgcgag aaggcgaggt
    12841 agccgtagaa cgagttgatg aggatcttga aggtctgctg gagcgcgttc aggaagatcc
    12901 gatcctcggg cgcggccgcc tcgcgggcca accgcttggc ggagacccgg aactcgcgga
    12961 ggtcacggag cagggaggcg gagaccccga gccgatcgga ggccgggaag agatcctgtc
    13021 cgagcatcag cgacgggtag agcgaggtca cgtccacgtg cagcacgtcg cgggccacgc
    13081 cctgctggaa caccgcggtg tagccccccg cgacgccgcg gccctggccg ggggcgggca
    13141 ccgcctggcg gcgatgcagg tactcccgca tcagcagcgc gtcgatcttg gtcgcgttac
    13201 cgcgcagcac cgtggtctgg tagtcgaagg gcagggcctg ggcctgcacg aagtagggtg
    13261 gcgacaggat ggccgagatc gcgagcgtct ccagcacgtc gtcgcgcgcg taggccatga
    13321 gccgcgccgg gtcctccacg aagatgcggg ggatgtcttc cggcgggagg taggtccggt
    13381 cggctgccgg cactccgaag tgccgcgcca cgtccttgag cccgtacgac tcgagatcgc
    13441 gcgcgcccac gtcgtagagc tgggccagga tccaggtgtc cacgatgtgg cggccggtca
    13501 ccccgtagcg ccggtagccg atcgcgcgct ccgccacctg catacgcgag ggatagccgc
    13561 gcagcacgga gccgtcgcgc ccccacgcga gcgggacgcg gtgccggcgg gcccgggcct
    13621 cgaggtactc gagatcgaag cggaagatgt tgtggccttc cagcacgtcg ggatcgcgct
    13681 cgcggaggat gcgcgagcac tcggccaaga gctgggcctc ggatagctcc gcacccgaca
    13741 gcacggtcga gaacccggtg gagtcggcca tcgcgatcgc gatgatgcgg tcggactcgc
    13801 gcgccgcgtt cgggaactcg aagccgggcg cggtggtgac ctcgatgtcg agcgccatgc
    13861 ggcgcaggtc gccgaagccc atgccgagga acgaggtctt cccggatcgc agcaggaact
    13921 ggtgcaccgg atcgccgagg aaccggtacg gggcgtcggg cgcccccgcg ctcttgccgg
    13981 agacgcgcag gcagtggtcg cgcacccgga gggcctgagg ccaggacggg cattcggcga
    14041 gccaccggta gacgccgtcg ccgtcgagcg gagtcacctc cacatcgccc ttgagctcct
    14101 tgagcagatc gggatcggcc aggagcagga acggccggaa gggctcgtcg gcggtgatca
    14161 cgaggcccga gcgaagggcg tagaccgtga cgtggctctc ggcggggcgg aaggcgagca
    14221 ggccgcgggc cgggtcgtgc ccgaagagca agggattgtc gtagaacggc agcgggatcg
    14281 aggtcaattg agcaggccgc ggagccggtg cagctccttc cgagcggtca cgtagcgcgg
    14341 ctcgatcgcc agcgcgcctt cgagttcccg gatggccgcg aagatctcgt gacgcttgat
    14401 gtggcttcgc gccaggttga agtacgcata gtgccgcggc tcgtagcgga ccgccttctt
    14461 cgcgcgctcg agccaggtca ccgcctcctc ctcgcgcccc agctcgatca ggtaggcgcc
    14521 gatgtcgttg taggggttcc cgaagtccgg gtccaccgag atcgcgatct tgcactgggc
    14581 gatcgcgtcg tcgggccgcc cctggtggct gtacgcccag ccgaggaagg tgtgggcctc
    14641 cgcggtcgga tgcagcgcga tggaccgctg gtagtgcgcc actgcctcct cgaggcgccc
    14701 ctgcatctgc gcctcgtaac cctgctggaa gtgccgatgc gcttccgcgc gcgtctccct
    14761 cgccggctcg tcgctcatcg gcccgactca ttcaccccta ggatgggctt gctcctcctg
    14821 gcggcattct accccagcgc ccgtgtgccg gcgcggcggc gtggtctcct ggtatgctcg
    14881 gcccgtgtac cggctcgcgg cagcggcgct ggcgacgacg ctcctcacgg gctgcgccgc
    14941 gcacaccgcg cgccagaccg tccatttccc caactccacc ccgggcgccc cggtgacgat
    15001 cgcggccacc ctggtccggc ccgccggccc cggcccgttt ccggcggtcg tgcagcttca
    15061 cggctgcgcc ggcctcgagg cgcagtcctt ccgctgggcg cgcgggctcg ccgagcgcgg
    15121 ctacgtggcc ctggtcgtgg acagcttcgg cccgcggggc gtcaagagcg actgccgctc
    15181 ggggccggac gatccaccga tcaccgcgcg cctcgacgac gccttcggag ccctgcgcta
    15241 tctgcaatcg cagacctatg t
//
LOCUS       DASUFA010000150         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1621714, whole genome shotgun sequence.
ACCESSION   DASUFA010000150 DASUFA010000000
VERSION     DASUFA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621714"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFX87_05350"
     CDS             complement(<1..486)
                     /locus_tag="VFX87_05350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4994370.1"
                     /translation="MNLFEQQRENRRRSVLLVAVFVLFFAWVGFGGDWAFWLLTRDAP
                     PGGYRHAVPWIGVIVCAAAAGIAAHSWTRGADRVLWSTGAWEVVEPATDAQRRLVNVV
                     EEMAIAAGVPRPRIWVVPDDDPNAFATGMTADRAHVAVTAGLLERLSRDELQAVVAHE
                     LS"
     gene            complement(493..1065)
                     /locus_tag="VFX87_05355"
     CDS             complement(493..1065)
                     /locus_tag="VFX87_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4994371.1"
                     /translation="MSTVILLVVVGAIVLWVISAYNRLITLRNRVANAWKQIDVQLKR
                     RHDLIPNLVEAVKGAMQFERQTMEAVISARTRAVSATGVAATARAEGELTQALGRLFA
                     VMEAYPDLKATGNISQLQEELTSTENKIGFARQHYNDEATLYNTAQALFPTNLVAGLA
                     KATPAELWEIEDAAERAVPKVDLSGGGAGA"
     gene            complement(1092..1784)
                     /locus_tag="VFX87_05360"
     CDS             complement(1092..1784)
                     /locus_tag="VFX87_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4994372.1"
                     /translation="MTASLALSGSAPDALGTLRAYRPLAPWGLRDLTALAAAILDASG
                     VVPVNASARARPSERTIRFYVARGLVSPPEGRGTAAVYSYRHLLQVLAIKLRQMEGAT
                     LETIIGEFTGQTGDSIERRVAGTLGAALPPPERLPLAQLPAMPRGRVGRAMQALAQSQ
                     PGRTPTGRPSICFRIPVGDGVEVLLDERHPAARSAAEAARLADRVRGALGEPPLAEPF
                     PSPASAPEPPPG"
     gene            2091..2528
                     /locus_tag="VFX87_05365"
     CDS             2091..2528
                     /locus_tag="VFX87_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994373.1"
                     /translation="MSKRGALLVELVLALVVAGLLVAIGVPALRAPLDRLATDRAALE
                     IAAAHTRARIASIAHNRVGRLFITQDSLTITLGHPPDTIRHWSAAGPAVSSVILIGPG
                     RELLFRPAGISFGVSNATYTLTRGAATTRVIISRLGRVRMVRQ"
BASE COUNT          403 a          980 c         1024 g          364 t
ORIGIN      
        1 cgagagctcg tgggccacca ccgcctgcaa ttcgtcccgg gagagccgct cgaggagccc
       61 ggcggtcacc gcgacgtggg cccggtcggc ggtcatcccg gtggcgaagg cgttggggtc
      121 gtcgtccggc accacccaga tccgcgggcg cgggacgccg gcggcaatgg ccatctcctc
      181 gaccacgttc accaggcgcc gctgggcgtc ggtggcgggc tcgaccacct cccacgcccc
      241 ggtggaccag agcacccgat cggccccacg cgtccaggaa tgggccgcga tgcccgcagc
      301 cgctgcgcag acgatcaccc cgatccacgg gaccgcgtgg cggtagccgc cgggcggggc
      361 gtcgcgggtc aggagccaga atgcccagtc gccgccgaag ccgacccagg cgaagaagag
      421 cacgaaaacg gcgacgagca ggacgctgcg ccgccggttc tcgcgctgct gctcgaagag
      481 gttcacgcgg gctcaggccc cggcgccacc gccggagagg tccaccttcg gcaccgcgcg
      541 ctctgcggcg tcctcgatct cccagagttc cgcgggcgtg gccttggcga gcccggccac
      601 gaggttggtg gggaacagcg cctgggcggt gttgtagagc gtggcctcgt cgttgtagtg
      661 ctgccgggcg aagccgatct tgttctcggt gctggtcagc tcttcctgga gctgggagat
      721 gttgccggtg gccttgaggt cgggataggc ctccatcacg gcgaagaggc ggcccagcgc
      781 ctgggtcagc tctccctcgg ccctcgcggt ggcggccacg ccggtggccg agacggcgcg
      841 ggtgcgcgcc gagatcaccg cttccatcgt ctgccgctcg aactgcatcg cccccttcac
      901 cgcctcgacc aggttgggga tcaggtcgtg ccgccgcttg agctggacgt cgatctgctt
      961 ccaggcgttg gcgacgcgat tgcggagcgt gatcaggcgg ttgtaggccg aaatgaccca
     1021 caacacgatc gcgcccacga cgacgaggag aatcaccgtg gacatcgcgg atgcctctct
     1081 gtggggggag atcagcccgg cggcggctcc ggggcggagg ccggcgacgg gaacggttcg
     1141 gcgagcggag gctcgcccag cgcgccgcgg acccggtccg cgagacgagc cgcctcggcg
     1201 gcgctccggg ccgccggatg ccgctcgtcc agcaacacct cgaccccgtc gcccaccggg
     1261 atccggaagc agatggacgg gcgcccagtg ggcgtccggc cgggctggga ctgcgccagc
     1321 gcctgcatcg cgcggcccac ccggccacgg ggcatcgccg ggagctgcgc gagcggcagg
     1381 cgctcggggg gcggcagtgc ggcgcccagc gtgcccgcca cccggcgctc gatggagtcg
     1441 ccggtctggc cggtgaactc gccgatgatg gtctcgagcg tggcgccttc catctggcgc
     1501 agcttgatgg ccagcacctg gagcaggtgg cggtacgagt acaccgccgc cgtgccccgg
     1561 ccctcgggcg ggctcaccag cccgcgggcg acatagaacc ggatggtgcg ctcgctcggg
     1621 cgagcccggg cggaagcgtt caccgggacc acccccgagg cgtcgaggat cgccgcggcg
     1681 agagcggtca ggtcgcgcag gccccacggc gccagcggcc ggtacgcgcg aagcgtaccg
     1741 agggcgtcgg gagcggaacc ggacaaggcc agggatgcgg tcatcggttg cggtgcgccg
     1801 ctcccggggg gcgggcggcg gttcgtcctc cgtctgacga cgccgcgcct cctcgggccg
     1861 caagtatcgc ggcgccccgg gagaccggca aggtgtgaca cttctgcgtc actgtcgtaa
     1921 gcagggagtg tgccgcaggc tcccggcctc ggactgccac cctaagccat tggcaggctt
     1981 gcgcttgtga cacgcctcac aggccgcgaa tcggcggcac cgcgttttcg cgtggcatcc
     2041 cgcgagagtc gggtgcagac ccggggcgtc cgggggcgca tcgtgcgccc atgtcgaagc
     2101 gcggcgccct cctcgtcgaa ctggtcctgg cactcgtcgt cgccgggctg ctggtggcga
     2161 tcggcgtgcc tgccctccgg gcgccgctcg accgcctggc caccgaccgg gccgcgctcg
     2221 agatcgccgc ggcacatacc cgggcgcgca tcgcctcgat cgcgcacaac cgggtgggcc
     2281 ggctcttcat cacccaggac tcgctcacca tcaccctcgg tcacccgccg gacaccatcc
     2341 gccattggag cgccgcgggc cccgcggtgt ccagcgtcat cctgatcggc ccgggccggg
     2401 agctcctgtt ccgtccggcc ggcatcagct tcggcgtctc caacgccacc tacaccctga
     2461 cgcgcggcgc tgcgaccacc agggtgatca tcagccggct cggccgggtg aggatggtgc
     2521 ggcagtaggc ggggaacggg aggcggtgag cggtgagcgg tgagcggtga gcggtgagcg
     2581 gtgagcgaat atcgaccagc gaacagcgaa cagcgaagag cgaacagcga acagcgaaga
     2641 gcgaaccacg aacagcgaag agcgaacttc caacagcgaa ccgcgacgtc ccggcaggac
     2701 ggcaaccgag ggcgaagcgg gcggctcggc ggcttcggcc gccgagtcgc agcccgctag
     2761 gcctgctcga t
//
LOCUS       DASUFA010000151        10180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_365837, whole genome shotgun sequence.
ACCESSION   DASUFA010000151 DASUFA010000000
VERSION     DASUFA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365837"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFX87_05370"
     CDS             <1..668
                     /locus_tag="VFX87_05370"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX4994374.1"
                     /translation="SGLRVLQPDWTALLWIVAAVTMTVGNVVALAQSNLKRMLAYSSI
                     AHVGYMLVGVVAGGSMGNGSVLFYLLVYTFTTAGAFGAVLLLERNGREAVQVADYGGL
                     ARRHPVLAVALSVFLLSLIGIPPTAGFVGKFYLFGAAVKSGYVWLAVIGVLNSAVAAY
                     YYLRVIVFMYMREPEGAPTVMAPSLSGALALVVALWGVVQLGVAPGPLFDLAQAAVIP
                     LLR"
     gene            712..1491
                     /locus_tag="VFX87_05375"
     CDS             712..1491
                     /locus_tag="VFX87_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX4994375.1"
                     /translation="MRPKLPYRGWLFDLDGTVYLGDHLIPGAAECLATLREAGRRVAF
                     LTNKPLQTRAEYAAKLTRLGVAAAPDDVINSSLVLARYLRDRDPGAPVFVIGEPPMRD
                     EMRAHGFEVRADERVRWVVIAFDRTFDYAKLNTALQAVKQGARLIATNPDRTCPVEGG
                     EIPDCAGMIAAVEAVTDRKVEVIVGKPSPIILDVALAALGVPAGEAVVVGDRIETDIV
                     MGRRLGLGTVLVLSGVTRADDPRIAAVAPDHVVRSIRDLVE"
     gene            1488..3125
                     /locus_tag="VFX87_05380"
     CDS             1488..3125
                     /locus_tag="VFX87_05380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX4994376.1"
                     /translation="MSAPANRLAGLDGAALDAVVIGGGMAGAGVARDLALRGASVALF
                     EKGDFASGTSSRSSKLIHGGLRYLELGDFKLVRESLREKKTLERLAPHLVRPLPFLVP
                     VYRGAARSLIKVRMGMWLYDLLTPGKNSERYRVLRPEATLSMEPSVRAEDLRGAGYYF
                     DDLLLFPERLCLENVLSAARHGARVLNYCQVEAFVPGARGLDGVQVRDLLSGQVQAVK
                     ARTIVNCAGPWVDRMRALAGVADRSPRLLRTTKGIHCLLPRMTDRAVYLSTHDERMIF
                     VIPWHGFSLVGTTDTDFDGDPDRLWATREEVTYLLDEVAKVLPDKRVAFENVAYTYAG
                     VRPLSFEPGRSASAVSREHRVVPEGPDGRFLSVTGTKLTCFRSLAEDVGDRVMRVLGR
                     KETSRTARAMLDGTDEEAGKIEVRAWMDVSEEMAATGLSRATLKTLVETYGRAFPRVL
                     DLGRKLPDGFERLCPSNPEIVAQLHHAVREELAVSLQDVLLRRTGIGQSRCQGLDCAE
                     PIAARMAELAGWTPRRLDAELEAYSQHVEQSRRFRHR"
     gene            3125..3487
                     /locus_tag="VFX87_05385"
     CDS             3125..3487
                     /locus_tag="VFX87_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994377.1"
                     /translation="MARLARGLVLLLVAALALGSVSRARAADDAWLLGRWELTRSPDG
                     DKTDWLEFAPDGRMTSITSDGRRLGGQYSVAGQEVQLNYKVGNQSILITLTYGSDKAQ
                     LYARSARTGYTAVYEKRP"
     gene            complement(3497..3871)
                     /locus_tag="VFX87_05390"
     CDS             complement(3497..3871)
                     /locus_tag="VFX87_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994378.1"
                     /translation="MIDIETRHRKNRQQAFKGLWWSDPTSIIRDPDGMMGRLLSPGQA
                     HDYWRHDEFDRLAGAARSTADGRARALAYRKMTAIFLEQNPWIVVLQPVEDYGLRRYV
                     DFAPNPDQQLELRRFNFRMRRG"
     gene            4081..4218
                     /locus_tag="VFX87_05395"
     CDS             4081..4218
                     /locus_tag="VFX87_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994379.1"
                     /translation="MGSLGTTPRPRQSSFTIVSRSMASDSAWRISGSFSGGTQTLTAS
                     T"
     gene            5230..6591
                     /gene="ugpB"
                     /locus_tag="VFX87_05400"
     CDS             5230..6591
                     /gene="ugpB"
                     /locus_tag="VFX87_05400"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEX4994380.1"
                     /translation="MPKTRKSWGWIVLALVLMGTATLGHYVQPARAQSPRVTVEFWHG
                     LPMPLGGILEKIVADFNESQTQYRVNATYKGSYPETMMGAIAAFRAGNAPHIVQMFDV
                     GTATMMSARGAIKPVHELMKESGVAFDPAAYLPAVAGYYSLPDGRMMAMPFNSSTAIM
                     FYNKDAFKKAGLDLNKPPQTWTELRAAAQKIRGANATPCGFTTAWPTWTQFEQFSAIH
                     NVPLATKANGLGGLDAELKINSPLHVRHVQTLIDMQKEGSFKYGGRDAAGDALFPSGE
                     CAIIHASSGLRARIAKEAKFEWGAAMLPYYPGTPDAPKNSIIGGAAFWVMTSPTRKPE
                     EYKAVAEFFRYISRPEVVAKWHTDTGFLPVTFAGYERVKASGFYQQNPGTDIPFLQMT
                     RTAPTENSRGLRLGNMPEIRNIIQEELEKAFQGQQSAQQALDNAVQRGNAVLRNFERA
                     NRS"
     gene            6621..7502
                     /gene="ugpA"
                     /locus_tag="VFX87_05405"
     CDS             6621..7502
                     /gene="ugpA"
                     /locus_tag="VFX87_05405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEX4994381.1"
                     /translation="MQRRVVFDGKVLPYLLLAPQLMVTIVFFFWPAGQAVYYSFLLQD
                     AFGLRQNFVWFDNYRVLLADPLYRGAAARTLVFTLAVTVLSMAPALLLAVMADKRIRG
                     AAAYKTLLIWPYAVAPAVAAVLWLFIFHPSIGVLGQMVRRWGVLWDYKLNGAQAMFLV
                     VLAASWKQVSYNFLFFLAGLQSIQKPVIEAAAIDGAGPARRFWSIIFPLLSPTTFFLL
                     VVNIVYVFFDTFGVIHALTGGGPGKATETLIYKVYVDGVVNLDLGGSSAQSVILMIFV
                     IAVTAVQFRYVERRVHY"
     gene            7502..8353
                     /gene="ugpE"
                     /locus_tag="VFX87_05410"
     CDS             7502..8353
                     /gene="ugpE"
                     /locus_tag="VFX87_05410"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEX4994382.1"
                     /translation="MVERRSRASWLPHAILLAGVIVFAFPIYTTVIGSTHDAGTIGRG
                     DLPLYPGAHGAENYTQAWTQGSGERAKGSPVARMMANSFLMAVVIAVGKIAVSLISAY
                     AVVFFAFPLRMFFFWMIFITLMLPVEVRIIPTFKVVSDLHLINTFGGLTLPLIASATA
                     TLLFRQFFMTIPDELVEAAKIDGAGPLRFFRHILLPLSRTNIAALFVILFLYGWNQYL
                     WPLLVTTSSGMNTIVIGITKMIGTGDAQTDWNLIMSTAMLAMLPPVAVILLMQRWFVK
                     GLVETEK"
     gene            8350..9177
                     /locus_tag="VFX87_05415"
     CDS             8350..9177
                     /locus_tag="VFX87_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX4994383.1"
                     /translation="MKGTLLLALGLVAVAAVVGSVTAAAQGMIAPLLAAHRGGALLWA
                     ENSLLAFRSALALGADFLELDVHLTRDGEVVVIHDATLDRTTTGTGPVRDRTLAELGA
                     LRLKDRGGAVLEERIPTLDQVVALAAAGKRQMLLEIKTDERGRRYPEIEEKVFAVLDR
                     HRFTPFTVVMAFEGDTWRRLRKLRPDARVAALYSARMLPAGAAAELEALRQAGVVFVG
                     LEQALVTAEVAKQARLAGLTLGVWTVNERDAIARFIDQGVGVVITDRPDLARELLGR"
     gene            9174..>10180
                     /locus_tag="VFX87_05420"
     CDS             9174..>10180
                     /locus_tag="VFX87_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX4994384.1"
                     /translation="MSARRFVLALDQGTTGSTALVIDPEGGVRARGYAELPQHYPQPG
                     WVEHDPGQIWATTVQALDQAVATSGITPAELAAVGITNQRETTILWERASGRPVGNAI
                     VWQCRRTAALCDRLKADGAEPEIRRKTGLVVDAYFSGTKIRWMLDHDPALRPRAERGE
                     LCFGTVDAWLLWQLTGGAVHATDPSNASRTLCFDIHRRAWDEGLCRLLGVPMALLPTV
                     KPSAGVFGETVGGRGAAVGGRSGYAKSAWLPPGIPVAGIAGDQQAALFGQACIEPGMA
                     KNTYGTGCFALLNTGERAVASREGLLTPVAWTVEGSTTYALEGAVFIAGAAVQWLRDG
                     LG"
BASE COUNT         1437 a         3601 c         3570 g         1572 t
ORIGIN      
        1 tgtccgggct ccgtgtcctg cagccggact ggacggcgct cctctggatc gtggccgccg
       61 tcacgatgac ggtcggcaac gtggtggccc tcgcccagtc gaacctcaag cgcatgctcg
      121 cctactcctc gatcgcccac gtgggctaca tgctggtcgg cgtggtggcg ggcggctcga
      181 tgggcaacgg cagcgtcctc ttctacctgc tcgtgtacac gttcacgacc gcgggcgcct
      241 tcggggccgt cctgctgctc gagcgcaacg gccgtgaggc ggtgcaggtc gccgactacg
      301 gcgggctggc ccggcggcat ccggtgctcg cggtggccct gtccgtcttc ctgctatcgc
      361 tgatcggcat cccgcccacc gcgggcttcg tgggcaagtt ctacctcttc ggcgccgccg
      421 tgaagtccgg ctacgtgtgg ctggccgtga tcggcgtgct gaactccgcg gtcgccgcct
      481 actactacct gcgcgtcatc gtcttcatgt acatgcgcga gcccgagggc gcgcccaccg
      541 tgatggcccc gtcgctctcc ggcgcactgg cgctcgtggt ggccctctgg ggcgtggtgc
      601 agctcggcgt ggcccccggc cccctcttcg acctggccca ggccgccgtc atcccgctgc
      661 tccgttgatc tccctctccc ctcaggggag agggcagggt gaggggtcct agtgcgcccc
      721 aagctcccct atcgcggctg gctcttcgac ctcgacggca ccgtctacct gggcgaccac
      781 ctgatccccg gcgcggccga gtgcctcgcg acgctccgcg aggccggccg tcgggtcgcc
      841 ttcctcacca acaagccgct ccagacccgc gcggagtacg cggccaagct cacgcgcctg
      901 ggggttgccg ccgcgcccga cgacgtcatc aactcctcgc tggtgctcgc gcgctacctg
      961 cgcgaccgcg acccgggcgc gccggtcttc gtgatcggcg agccgccgat gcgggacgag
     1021 atgcgcgccc acggcttcga ggtgcgggcg gacgagcgcg tgcgctgggt ggtgatcgcc
     1081 ttcgaccgca ccttcgatta cgcgaagctc aacaccgcgc tccaggcggt gaagcagggc
     1141 gcgcgcctca tcgccaccaa ccccgaccgc acctgcccgg tggagggcgg cgagatcccg
     1201 gactgcgcgg gcatgatcgc cgcggtggaa gcggtgaccg accgcaaggt ggaggtgatc
     1261 gtcgggaagc cgtcgccgat catcctggac gtcgcgctgg ccgcgctcgg cgtccccgcg
     1321 ggcgaggcgg tggtggtggg cgaccgcatc gagaccgaca tcgtgatggg ccggcggctc
     1381 gggctcggca ccgtgctcgt gctgagcggc gtgacgcgcg cggatgaccc gcgaatcgcg
     1441 gcggtcgctc cggaccacgt ggtacgatcc atccgggacc tggtggagtg agcgcgcccg
     1501 cgaatcggct ggctggactg gacggcgccg cgctggacgc cgtggtgatc ggcggcggca
     1561 tggcgggggc gggagtggcg cgcgatctgg ccctgcgcgg cgcctcggtc gcgctgttcg
     1621 agaagggcga cttcgcctcc ggcacctcgt cgaggtcctc caagctgatc cacggtgggc
     1681 tccgctacct cgagctggga gacttcaagc tggtgcgcga gtcgctgcgc gagaagaaga
     1741 cgctcgagcg cctcgccccg cacctggtgc gcccgctgcc cttcctggtg ccggtgtacc
     1801 gcggcgccgc gcgcagcctc atcaaggtgc gcatgggcat gtggctctac gacctgctga
     1861 cccccggcaa gaacagcgag cgctaccgcg tgctgcggcc cgaggccacg ctctcgatgg
     1921 agccgagcgt gcgggcggag gacctgcgcg gggccggcta ctacttcgac gacctgctgc
     1981 tcttcccgga gcgcctctgc ctcgagaacg tgctctcggc ggcgcgccac ggggcgaggg
     2041 tcctcaacta ctgccaggtc gaggcgttcg tcccgggcgc ccgcgggctc gacggcgtgc
     2101 aggtgcgcga cctcctctcg ggccaggtgc aggcggtcaa ggcgcgcacc atcgtgaact
     2161 gcgcggggcc gtgggtggat cgcatgcgcg cgctcgcggg cgtggccgac cggagcccgc
     2221 ggctgctgcg caccaccaag ggcatccact gcctcctgcc gcgcatgacc gaccgcgcgg
     2281 tgtacctctc cacccacgac gagcggatga tcttcgtcat cccgtggcac ggcttctcgc
     2341 tggtcggcac caccgacacc gacttcgacg gcgatccgga ccgcctctgg gcgacccgcg
     2401 aggaggtgac ctacctgctc gacgaggtcg ccaaggtgct gccggacaag cgcgtcgcct
     2461 tcgagaacgt ggcctatacc tacgcgggcg tgcggccgct ctccttcgag ccgggccgct
     2521 ccgcctccgc ggtctcgcgc gagcaccgcg tggtgccgga ggggccggac ggccgcttcc
     2581 tctccgtcac cggcaccaag ctgacctgct tccgcagcct ggccgaggac gtgggggatc
     2641 gcgtgatgcg cgtgctgggc cgcaaggaga cctcgcgcac cgcgcgcgcg atgctcgacg
     2701 gcaccgacga ggaggccggc aagatcgagg tgcgcgcgtg gatggacgtg tcggaggaga
     2761 tggcggcgac cgggctctcg cgcgccacgc tcaagacgct ggtggagacc tacgggcggg
     2821 cattcccccg cgtgctcgat ctgggccgca agctgcccga cggcttcgag cgcctctgcc
     2881 cgagcaatcc cgagatcgtg gcccagctcc accacgcggt gcgcgaggag ctcgcggtat
     2941 cgctgcagga cgtgctgctg cggcgcaccg gcatcggcca gagccgctgc cagggcctcg
     3001 actgcgcgga gccgatcgcc gcgcgcatgg ccgagctggc gggctggacg ccgcgccgcc
     3061 tcgacgccga gctcgaggcg tattcgcagc acgtcgagca gagccggcgg ttccggcacc
     3121 gctgatggcc cgtctcgccc gcggtctcgt gctcctcctg gtcgcggcat tggcgctcgg
     3181 ctcggtctcg cgggcgcgcg cggccgacga cgcgtggctg ctcggccgct gggagctgac
     3241 gcgctcgccg gacggcgaca agacggactg gctcgagttc gccccggacg ggcggatgac
     3301 ctcgatcacg tcggacggcc ggcgcctggg cggccagtac tccgtcgccg gccaagaggt
     3361 ccagctcaac tacaaggtcg gcaaccagtc catcctgatc acgctcacct acgggtccga
     3421 caaggcgcag ctctacgccc gctccgcccg gaccggctac accgcggtct acgagaagcg
     3481 gccctgacct cggcgatcag ccgcggcgca tccggaagtt gaagcggcgc agctcgagct
     3541 gctggtctgg attcggggcg aagtccacgt agcgccgcag gccgtagtcc tccaccggct
     3601 gcagcaccac gatccacgga ttctgctcga ggaagatcgc ggtcatcttc cggtaggcca
     3661 aggcccgggc gcgcccgtcc gcggtggatc gcgcggcgcc ggccaggcgg tcgaactcgt
     3721 cgtggcgcca gtagtcgtgc gcctgccctg ggctgagtag ccgccccatc atgccgtcgg
     3781 ggtcgcggat gatggacgtc gggtccgacc accagagtcc cttgaacgcc tgctgccggt
     3841 tcttccggtg gcgggtctcg atgtcgatca cgtcgaggac gaccttgacg ccgacgtcct
     3901 ccctcccaca tctcggcgat ggcctcggcc atgagccgat cgttcgcgat catgcccgcc
     3961 gtcgtctcga agtggatcgc ctcgccccgg tagccggccc gccgcagtcg ctcccgcgcc
     4021 gcgccgggat cgtagggcag cgcccgcagc gagggatcgt ggagatcatc gcctcgcggg
     4081 atgggttcac tcggcaccac gccgcggccg cgccagagct ccttcacgat cgtctcccgg
     4141 tcgatggcga gcgacagcgc ctggcggatc agcgggtcgt tcagcggcgg cacccagacg
     4201 ttgaccgcga gcacgtagag accggcatag agcacgccgg ccacctgggt ggaggggtcc
     4261 gacgccactc gaggaccatg ctcgggggcc agcggcacga tcaggtcggc gccgccgcga
     4321 agcagcaggt ctgcccgccg ctggtggtcc gacaccgact tgatcaccac ccgatccaca
     4381 tcgacgcggc catcccagta gtcgaggttg gcgtcgagga cacactgatc gcccttgctc
     4441 cgcgacacga agcggagcgg gccgctgccg accggccgct cgttgaaggc cttgaagccc
     4501 acccggtcca tgtacgccca cgggacgact gcgcccgcgc acgacgccag acgggccggc
     4561 atgagcagat cgggccaccg cgtgtggatg atcagcgtcg ccgggtcggg cgcctcggtc
     4621 cgatcgatcg cctgggtcca ccaggccccg agcaggcggg cccccttgac ggtggcgtcg
     4681 taggtgcggt cgaggctgta cttggcgtcg accgaggtga agcgggttcc atcgtgccag
     4741 cgcacgtcgg agcgcagcgc gagctgccac gtcgtggggg tcgttcgctt ccaggcgctc
     4801 gcgagcgccg ggtgcagcgt cccgtcggga tggcgccgga tgagggtgtc gaagacgttg
     4861 aacgcgattc gccagtcgct cgacagggtg gacgcgtggg gatcgaggct ggtcaggtcg
     4921 tacgcctgcg cgatgaccag atcgcgcttg gaggtcgtct gcgtgtcgag cgccgcggag
     4981 gacgcagcgc ggggtatcgc cggcgcgagt gcggcggcca ctccaaagga ggcgcgcgag
     5041 aggaagcccc ggcggtccac ccgctcgcga atagagcaac ttcccggccg cgagtcaata
     5101 gtgctatatt gcacgcgctc gtcggccgat acggagatgt catccgggcg cagcccggtc
     5161 gagacgcctg ttctatagcc tgagccagac atggaccgcg aggtcccgat caacaaccgg
     5221 agggcaccca tgccaaagac aaggaagtcc tgggggtgga tcgtgctggc gctcgtgctg
     5281 atgggcaccg cgacgctggg gcactacgtg cagccggcgc gggcccagtc cccacgcgtg
     5341 acggtggagt tctggcacgg gctgcccatg ccgctcggcg gcatcctcga gaagatcgtc
     5401 gccgacttca acgagtcgca gacccagtac cgggtcaacg ctacctacaa gggctcgtac
     5461 cccgagacca tgatgggggc gatcgccgcg ttccgggcgg gcaacgcgcc gcacatcgta
     5521 cagatgttcg acgtgggcac cgcgaccatg atgtcggcgc gcggggcgat caagccggtg
     5581 cacgagctga tgaaggagtc cggggtggcg ttcgatcccg ccgcgtacct gcccgcggtg
     5641 gccggctact acagcctgcc cgacgggcgg atgatggcga tgccgttcaa cagctcgacc
     5701 gcgatcatgt tctacaacaa ggacgccttc aagaaggcgg ggctcgatct gaacaagccg
     5761 ccgcagacct ggaccgaact gcgggcggcg gcccagaaga tccgcggggc caacgcgacg
     5821 ccgtgcggct tcaccaccgc gtggccgacg tggacgcagt tcgagcagtt cagcgccatc
     5881 cacaacgtgc cgctcgcaac caaggccaac ggcctgggcg ggctcgacgc cgagctcaag
     5941 atcaacagcc cgctgcacgt gcgccacgtg cagaccctga tcgacatgca aaaggagggg
     6001 agcttcaagt acggcggccg cgacgcggcg ggcgacgcgc tcttcccctc cggggagtgc
     6061 gcgatcatcc acgcctcgtc cgggctgcgc gcccgcatcg cgaaggaggc caagttcgag
     6121 tggggcgcgg ccatgctgcc ctactacccg ggcacgcccg acgcccccaa gaactcgatc
     6181 atcggcgggg ccgccttctg ggtgatgacc tcgcccaccc gcaagcccga ggagtacaag
     6241 gcggtcgccg agttcttccg ctacatcagc cgccccgagg tcgtggccaa gtggcacacc
     6301 gacaccggct tcctccccgt caccttcgcg gggtacgagc gggtgaaggc ctccggcttc
     6361 taccagcaga acccgggcac cgacattccg ttcctccaga tgacccggac cgcgcccacc
     6421 gagaactcgc ggggcctgcg gctcgggaac atgccggaga tccggaacat catccaggag
     6481 gagctggaga aggcgttcca ggggcagcag tcggcgcagc aggccctcga caacgcggtg
     6541 cagcggggga acgcggtcct gcgcaacttc gagcgggcga accgctcgta gccgggaggg
     6601 cacacggccg ctgaccggcc atgcagcgcc gggtcgtctt cgacggcaag gtactcccct
     6661 acctgctcct ggcgccgcag ctcatggtca cgatcgtctt cttcttctgg ccggccgggc
     6721 aggcggtcta ctactcgttc ctgctgcagg acgccttcgg cctgcggcag aacttcgtct
     6781 ggttcgataa ctaccgcgtc ctcctcgccg acccgctgta ccggggcgcg gcggcccgca
     6841 cgctcgtctt cacgctcgcg gtcaccgtgc tgtccatggc gccggcgctg ctgctcgcgg
     6901 tcatggccga caagcgcatc cgcggggccg ccgcctacaa gaccctgctg atctggccct
     6961 acgcggtggc cccggcggtg gcggcggtgc tctggctctt catcttccac ccgtccatcg
     7021 gggtgctggg ccagatggtg cgcaggtggg gcgtcctctg ggactacaag ctcaacgggg
     7081 cccaggcgat gttcctggtg gttctcgccg cgagctggaa gcaggtgagc tacaacttcc
     7141 tcttcttcct ggccgggctg cagtccatcc agaaaccggt gatcgaggcc gccgccatcg
     7201 acggggccgg cccggcccgg cgcttctgga gcatcatctt cccgctgctc tctccgacga
     7261 cgttctttct tctggtcgtc aacatcgtgt acgtcttctt cgacaccttc ggcgtcatcc
     7321 acgccctcac cggcggcggg cccgggaaag ccaccgagac cctcatctac aaggtctacg
     7381 tggacggggt ggtcaacctg gatctgggcg gctcctcggc ccagtcggtc atcctgatga
     7441 tcttcgtcat cgcggtcacc gccgtgcagt tccgctacgt ggagcggcgg gtccactact
     7501 gatggtcgag cgccggtcac gcgcctcctg gcttccccac gcgatcctcc tggccggggt
     7561 gatcgtcttc gccttcccca tctacaccac ggtgatcggc tcgacccacg acgcggggac
     7621 catcgggcgc ggggacctgc cgctctaccc gggcgcgcac ggcgcggaga actacacgca
     7681 ggcgtggacg cagggaagcg gggagcgcgc caagggctct ccggtggcgc gcatgatggc
     7741 caatagcttc ctgatggcgg tggtcatcgc ggtgggcaag atcgcggtgt cgctgatctc
     7801 ggcctacgcg gtggtcttct tcgcgttccc gctccgcatg ttcttcttct ggatgatctt
     7861 catcaccttg atgctgccgg tggaggtgcg gatcatcccg acgttcaagg tggtgtccga
     7921 tctccacctg atcaacacct tcggcggcct caccctcccc ctgatcgcct ccgccaccgc
     7981 gaccctgctc ttccggcagt tcttcatgac catcccggac gagctggtgg aggcggccaa
     8041 gatcgacgga gccggccccc tccgcttctt ccgccacatc ctgctgccgc tgtcgcggac
     8101 caacatcgcg gccctgttcg tcatcctctt cctctacggc tggaaccagt acctgtggcc
     8161 gctcctcgtc acgaccagca gcggcatgaa caccatcgtc atcggcatca ccaagatgat
     8221 cggcaccggc gacgcccaga ccgactggaa cctgatcatg tccaccgcga tgctcgccat
     8281 gctgccgccg gtcgccgtca tcctgctgat gcaacggtgg ttcgtgaagg gcctggtgga
     8341 gaccgagaag tgaaggggac gctcctgctg gcgctgggcc tggtggcggt ggccgcggtc
     8401 gtgggctccg tgaccgcggc cgcgcagggc atgatcgccc cgctcctcgc cgcgcaccgg
     8461 ggcggcgcgc tgctctgggc ggagaacagc ctgctcgcct tccgcagcgc gctcgccctg
     8521 ggcgccgact tcctcgagct cgacgtgcat ctcacgcgtg acggcgaggt ggtggtgatc
     8581 cacgatgcca cgctcgaccg caccaccacc ggcaccggcc cggtgcggga ccgcacgctc
     8641 gcggagctgg gcgccctgcg cctcaaggat cgcggagggg cggtgctcga ggagcgcatc
     8701 cccacgctcg accaggtggt ggcgctggcc gcggcgggga agcggcagat gctgctcgag
     8761 atcaagaccg acgagcgggg gcgtcgctac ccggagatcg aggagaaggt gttcgcggtg
     8821 ctcgaccgcc accgcttcac gcccttcacg gtcgtcatgg ccttcgaggg cgacacgtgg
     8881 cggcggctgc gcaagcttcg gcccgacgcg cgggtggccg cgctctactc ggcgcgcatg
     8941 ttgccggccg gcgccgccgc ggagctcgag gcgctgcgcc aggccggcgt cgtcttcgtc
     9001 ggcctcgagc aggcgctggt gaccgccgag gtggccaagc aggcgcggct ggccggtctc
     9061 accctcgggg tgtggacggt gaacgagcgc gacgccatcg cgcgcttcat cgaccagggg
     9121 gtgggcgtgg tcatcacgga tcgcccggac ctggcccggg agctgctcgg ccggtgagcg
     9181 cgcgccgctt cgtcctggcg ctggaccagg ggaccaccgg gtccaccgcg ctcgtgatcg
     9241 atccggaagg cggcgtgcgc gcgcgcggct acgcggagct gccccagcat tacccgcaac
     9301 cgggctgggt ggagcacgat ccggggcaga tctgggccac caccgtgcag gccctcgacc
     9361 aggcggtggc cacctccggg atcacgccgg ccgagctcgc ggcggtcggc atcaccaacc
     9421 agcgcgagac caccatcctg tgggagcgcg cctccggtcg cccggtgggc aatgccatcg
     9481 tctggcagtg ccggcgcacc gccgcgctct gcgatcggct gaaggccgac ggggccgagc
     9541 ccgagatccg gcggaagacc ggcctggtgg tggacgcgta cttctcgggc acgaagatcc
     9601 gatggatgct cgaccacgat ccggcgctgc gcccgcgcgc ggagcgcggc gagctgtgct
     9661 tcgggacggt ggatgcgtgg ctgctctggc agctcaccgg tggagccgtg cacgcgaccg
     9721 atccgtccaa cgcctcgcgc acgctctgct tcgacatcca tcgccgggcg tgggacgagg
     9781 gcctgtgccg gctgctgggc gtgccgatgg cgctgctgcc gaccgtgaaa ccgtcggccg
     9841 gcgtgttcgg cgagaccgtc ggcgggcgag gcgccgccgt gggcgggcgg agcggatatg
     9901 ccaaatccgc gtggctgccg ccgggcattc cggtggcggg catcgcggga gatcagcagg
     9961 ccgcgctctt cggccaggcg tgcatcgagc ccggcatggc caagaacacg tacgggaccg
    10021 gctgcttcgc gctgctgaac accggcgagc gcgccgtcgc ctcccgcgag ggcctcctga
    10081 ccccggtggc gtggacggtg gaggggagca cgacctacgc gctcgagggc gcggtgttca
    10141 tcgcgggcgc ggcggtgcag tggctgcgcg acggcctcgg
//
LOCUS       DASUFA010000152         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_722445, whole genome shotgun sequence.
ACCESSION   DASUFA010000152 DASUFA010000000
VERSION     DASUFA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722445"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VFX87_05425"
     CDS             complement(<1..1275)
                     /locus_tag="VFX87_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4994385.1"
                     /translation="MGAKYFGASVRRKEDPRYLRGEGRFVDDIKLPGMLHAAFVRSPH
                     AHARIAAIRTDAAKRLPGVAHVLTFADLERWMKPLPLFGAIPPGLAARVVVSMKQPHQ
                     LAMCRDEARHVGEIVAMVLASSRAVAEDGCELVEVDYEPLPVLADVEAAARPDAPVLY
                     PAWGDNVALSFKTGFGDVEAAFRQADARVRERFVIPRYVGMPIETRGVVARWDPRDGT
                     LTTWNGTQVVHFVQQGLVAALGLPPHKIRVIAPDVGGGFGTKANGYPEDLLIPAAAIL
                     ARRPVKWTEDRREHMMGSAHARAQVHDIEIAARRDGTMLAVRDRIWVDLGAYNSWGIV
                     LPYNTVAHLLGPHRVGNLDVECLGVVTTKTPNAPYRGAGRPETVFAMDRAVDGLARQL
                     GLDPAEIRRRNYLTARELPWDLGMPYRDGNPLV"
     gene            complement(1406..2110)
                     /locus_tag="VFX87_05430"
     CDS             complement(1406..2110)
                     /locus_tag="VFX87_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994386.1"
                     /translation="MLTLERLSARYGRIAALDSVSLTVGPAEFVCLIGANGAGKTTTL
                     KTISGLVRAAAGRIVFDGQEIQHLAPQEILRRGIAHCPEGRRVFPHMTVEENLQMGAY
                     VRTDSAAVTADRDRVFGHFPILAERRRQAAGTLSGGEQQMLAIGRCLMARPRLILFDE
                     PSLGLAPTVTETVFQIIAGIQREGTPVLMVEQNAYMALQLASRAYVMETGRITLEGPA
                     QDLMKNDHVKRAYLGG"
     gene            complement(2097..2867)
                     /locus_tag="VFX87_05435"
     CDS             complement(2097..2867)
                     /locus_tag="VFX87_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994387.1"
                     /translation="MTLEARDLTVRFGGVAALTGVSFAVAPGTVTSLIGPNGAGKTTA
                     FNVITGFQRATQGTVHHDGQPITGWRPHRIAARGLVRTFQKTSLFPGLTVRENVLTGL
                     HLRGRVGVAAALLARGRVRDEERRLGLEADHVMDFVGLGARRGEAAAALSYGEQRLLE
                     LAVGLAARPRLLLLDEPASGMTASEKAVVATLIGRIRDEGITVFLVEHDMRLVMGISD
                     RVLVLNYGRLIADGDPAAVQRDPEVIRAYLGAGAHAHP"
     gene            complement(2864..3829)
                     /locus_tag="VFX87_05440"
     CDS             complement(2864..3829)
                     /locus_tag="VFX87_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994388.1"
                     /translation="MRRLALGIGMGIVLTAPAWVWNPYHLHTLIMAGIFAVLALSLNL
                     LLGYTGQLSLGHAAFFGIGAYASALLSVKLEWSPWIGLLAAIVAPGLAGYVIGRLALK
                     LRGAYFVLLTISFAGCVSLVSVNWMDLTNGPLGIPGVPPVEIALPGLSALSLRTKSAY
                     YYLVLAAVVGAYLLCLALIRSRVGRALVALRENETLAASVGIDATHYLVLAAVTSAAM
                     AGFAGGLYAHYTRFVSPEVFLFTYTVTMVIMVVAGGKGTLAGPVVGAVLFTVLPEALR
                     ELTSYQWQMLLYGVLLIAVLFFMPEGIVPALRAAGPALRRRRRTS"
     gene            complement(3826..>4088)
                     /locus_tag="VFX87_05445"
     CDS             complement(3826..>4088)
                     /locus_tag="VFX87_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994389.1"
                     /translation="ALLGPVFLLYPSMGDLASLKAFSVVILGGLGNFGGAALGGLVLG
                     IAEELGAGYISSGYRDAVGFVMIVAVLLLRPSGLFARTERVG"
BASE COUNT          624 a         1490 c         1447 g          527 t
ORIGIN      
        1 cacgagcgga ttgccgtcgc ggtacggcat gccgaggtcc cacggcagct cgcgcgcggt
       61 gaggtagttg cggcggcgga tctcggcggg gtcgagcccg agctggcgcg ccagcccgtc
      121 gaccgcgcgg tccatcgcga acacggtctc ggggcggccc gcgccgcgat agggcgcgtt
      181 cggcgtcttg gtggtcacca ccccgaggca ctcgacgtcg aggttcccca cgcgatgcgg
      241 gcccagcagg tgcgccaccg tgttgtaggg cagcacgatg ccccaggagt tgtaggcgcc
      301 caggtccacc cagatacgat cgcgcaccgc cagcatcgtc ccgtcccgcc gggccgcgat
      361 ctcgatgtcg tgcacctggg cgcgggcgtg ggccgagccc atcatgtgct cgcggcggtc
      421 ctccgtccac ttcaccggcc ggcgcgccag gatggcggcg gccggaatca gcaggtcctc
      481 cgggtagccg ttggccttgg tgccgaagcc gccgcccacg tccggggcga tgacacggat
      541 cttgtggggc ggtagcccga gcgcggccac cagcccctgc tggacgaagt ggacgacctg
      601 ggtgccgttc cacgtggtga gcgtgccgtc gcgcggatcc caccgggcga ccacgccgcg
      661 cgtctcgatc ggcatgccca cgtagcgcgg gatcacgaag cgctcgcgca cccgcgcgtc
      721 ggcctgccgg aaagccgcct cgacgtcgcc gaagccggtc ttgaacgaga gcgccacgtt
      781 gtcgccccag gcgggataga gcacgggggc gtcgggccgc gcggccgcct ccacgtcggc
      841 cagcaccggc agcggctcgt agtccacctc gaccagctcg cagccgtcct cggccaccgc
      901 ccggctgctg gccagcacca tcgcgacgat ctcgcccacg tggcgcgcct cgtcccggca
      961 catcgcgagc tggtgaggct gcttcatgct gacgaccacg cgcgcggcca ggccgggcgg
     1021 gatcgcgccg aagagcggca gcggcttcat ccagcgctcg agatcggcga aggtgagcac
     1081 atgcgccacg cccgggagcc gcttggccgc gtcggtgcgg atggccgcga tacgcgcgtg
     1141 cgcgtgcggg ctgcggacga aggccgcgtg gagcatcccg ggcagcttga tgtcgtccac
     1201 gaagcggccc tcgccgcgga ggtagcgcgg gtcttctttg cgtcttaccg atgctccgaa
     1261 gtacttggcg cccatgtttc gctcggcctc tctcccagtc cgcggacaac cgttcactcc
     1321 ttcaggctct tcagctgcgg gggcccagac atggcccccc gcactccccc actcggcgga
     1381 agcccgtttc ggcggttgga ctcggctagc cgcccaagta tgccctcttc acgtggtcgt
     1441 ttttcatgag gtcctgcgcg ggcccctcca gggtgatgcg gcccgtttcc atgacgtagg
     1501 cgcggctggc gagctggagc gccatgtagg cgttctgctc gaccatcagc acgggggtgc
     1561 cttcgcgctg gatgcccgcg atgatctgga acacggtctc cgtgaccgtg ggggccagcc
     1621 cgagcgaggg ctcgtcgaac aggatgaggc gcgggcgcgc catgaggcaa cgcccgatgg
     1681 cgagcatctg ctgctcgccg cccgagagcg tgcccgcggc ctgccggcgc cgctccgcca
     1741 ggatcgggaa gtggccgaag acccggtccc ggtccgccgt caccgccgcg ctgtcggtgc
     1801 ggacgtaggc gcccatctgg aggttctcct ccacggtcat gtgcgggaac acgcgccggc
     1861 cctccgggca atgcgcaatg ccccggcgca ggatctcctg cggggccaga tgctggatct
     1921 cctgcccgtc gaagacgatg cggccggcgg ccgcccgcac gagccccgag atggtcttga
     1981 gcgtggtggt cttgcccgca ccgttggcgc cgatgaggca gacgaactcc gcggggccca
     2041 ccgtgagcga caccgagtcg agcgcggcga tgcgcccgta gcgcgcggag agccgctcaa
     2101 gggtgagcat gggctcccgc tcccaggtag gcgcggatca cctccgggtc gcgctgcacc
     2161 gcggccgggt cgccgtcggc gatgagccgg ccgtagttca gcaccagcac gcggtccgag
     2221 atgcccatca ccaggcgcat gtcgtgctcg accaggaaca ccgtgatgcc ctcgtcgcgg
     2281 atgcggccga tcagcgtggc caccaccgcc ttctcggagg cggtcatccc cgaggccggc
     2341 tcgtcgagca gcagcaagcg gggccgcgcg gccagcccga ccgccagctc cagcagccgc
     2401 tgctcgccgt aggagagcgc ggcggccgcc tccccgcggc gcgcccccag cccgacgaag
     2461 tccatcacgt gatccgcctc caggccgagc cgccgctcct cgtcccgcac ccggccgcgc
     2521 gccagcagcg ccgccgccac acccacgcgc ccgcgcagat gcaggcccgt gagcacgttc
     2581 tcgcgcaccg tcaggccggg gaagaggctc gtcttctgga acgtgcgcac gaggccgcgc
     2641 gccgcgatgc gatgaggccg ccagccggtg atcggctggc cgtcgtggtg cacggtgccc
     2701 tgggtcgcgc gctgaaaccc ggtgattaca ttgaacgcgg tggttttccc cgcgccgttg
     2761 gggccgatca ggctcgtcac cgtgccgggg gcgaccgcga agctcacgcc ggtgagcgcg
     2821 gccacgccgc cgaagcgcac cgtgaggtcc cgcgcctcga gcgtcacgac gtccgccggc
     2881 gccggcggag ggccggcccg gccgcccgca gggcgggaac aatcccctcc ggcatgaaga
     2941 acagcaccgc gatgagcagg acgccgtaga gcagcatctg ccactggtac gaggtcagct
     3001 cgcgcagcgc ctccggcagc accgtgaaga gcacggcgcc caccacgggc ccggccagcg
     3061 tgcccttccc gcccgccacc accatgatca ccatcgtgac cgtgtaggtg aagaggaaga
     3121 cctccgggct gacgaagcgc gtgtagtgcg cgtagaggcc gcccgcgaag ccggccatcg
     3181 ccgcgctcgt caccgcggcc aggacgagat agtgggtcgc gtcgatcccc accgaggcgg
     3241 cgagcgtctc gttctcccgg agcgctacca gcgcccggcc gacccgcgag cggatgaggg
     3301 ccaggcacag gaggtaggcc ccgaccaccg cggccagcac caggtagtag taggcgctct
     3361 tggtgcgcag cgacagcgcg gagagcccgg gcagcgcgat ctccaccggg ggcactcccg
     3421 ggatgccgag cggcccgttg gtcaagtcca tccagttcac gctcaccagc gagacgcagc
     3481 ccgcgaagga gatggtcagc agcacgaaat aggcgccgcg cagcttgagc gcgagccggc
     3541 cgatgacgta gccggccagg cccggcgcca cgatggcggc cagcaggccg atccagggcg
     3601 accactcgag cttgacgctc agcagggcgg aggcgtaggc gccgatgccg aagaaggcgg
     3661 cgtggccgag cgagagctgt ccggtatagc ccagcaggag gttcaggctc agcgccagca
     3721 ccgcgaagat gccggccatg atcagcgtgt gcaggtgata cgggttccac acccacgcgg
     3781 gcgcggtcag gacgatgccc atgccgatcc cgagcgcgag gcgcctcatc ccacgcgctc
     3841 ggtgcgggcg aagaggcccg acggccgcag cagcagcacc gcgacgatca tcacgaagcc
     3901 caccgcgtcg cggtagccgg aggagatgta gccggcgccc agctcctccg cgataccgag
     3961 caccaggccg cccagcgcgg cgccgccgaa attgcccaga ccgcccagga tgaccaccga
     4021 gaaggccttc agcgaggcca gatcgcccat ggacgggtag aggagaaaga ccgggcccag
     4081 cagcgcgc
//
LOCUS       DASUFA010000153         6717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_837970, whole genome shotgun sequence.
ACCESSION   DASUFA010000153 DASUFA010000000
VERSION     DASUFA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837970"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFX87_05450"
     CDS             complement(<1..651)
                     /locus_tag="VFX87_05450"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX4994390.1"
                     /translation="MAATASQVVTIIADDLTGACDTGCLFAGPGPVGVLAPPALTAAD
                     SSTIMPVITIDTESRALPYREAAAAIHHAARQLGGRLAAGHVFKKIDSTLRGAVGAEL
                     TALLEYGPPFTGALVCPAFPAQHRVVRRGRLLVDSVPVHESAITRDPAFRAATSDVAA
                     LLAGGAPVVTLGLDDVRAGAEKVAHLLERHRGALVAADAETDDDLATLAEAALAAPG"
     gene            738..1268
                     /locus_tag="VFX87_05455"
     CDS             738..1268
                     /locus_tag="VFX87_05455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4994391.1"
                     /translation="MKSRVVVALLALAAVLAPGGAAGEDPVAALSLIRPKPAKDAPDF
                     ELRTPDDRVIRLADLKGKVVFLNFWATWCEPCREEMPGMERLHLAYKDRGLVVLAVSL
                     DSQGASVVIPFVKKFGLTFLIGLDPKMAVRERYGVWAVPSTFLIDRKGKRVLFANGAR
                     EWNGKAAHAVIESLLK"
     gene            1265..2002
                     /locus_tag="VFX87_05460"
     CDS             1265..2002
                     /locus_tag="VFX87_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX4994392.1"
                     /translation="MSESLGVLVAFSAGLFSFLSPCVLPLFPSYLSFITGMSVDRLTH
                     EVTAGARTRVLVHSLAFIVGFSVVFVSLGASFSAAGQFLLDYREWIRIAGGALIIVFG
                     LYIAGVLRIGIFGRTQQLQIQSKPAGLLGSFLVGLTFAIGWTPCVGPILGSILTLASN
                     DKTVGQGVTLLLFYSAGLGLPFLLSSVALGGFLRFFKHYRPFIPTVERAAGVLLVLVG
                     ILVATNYYVLLNSWAISLTPEWLLRRL"
     gene            2021..2290
                     /locus_tag="VFX87_05465"
     CDS             2021..2290
                     /locus_tag="VFX87_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994393.1"
                     /translation="MVLHRIGPLSAAKVVAVLYAIFGLIMGAFLSVAALFRADGGGTA
                     PLWGVAAVVFFPVLYGVGGFIVTLITAWPYNVVAGAVGGVELDLR"
     gene            complement(2326..3027)
                     /locus_tag="VFX87_05470"
     CDS             complement(2326..3027)
                     /locus_tag="VFX87_05470"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4994394.1"
                     /translation="MPEPQGPALPESVRVAVASGTVSALVYPPPEPSPALATLILAHG
                     AGAPQSSAFMVAFAQGIARRGCHAVTFNFPYTEQGRRLPDRAPTLEACFRDVIAAIRG
                     RADLAAAPLVIGGKSMGGRIASHLAAQGVEDLAGLVALGYPLHPPGRPEQLRAEHLAR
                     IRRPMLIVQGSRDAFGTPEELRPALAPLGTTATLQVVAGGDHSFKVPKRGPISQEEVF
                     ERVQDEIARWIRRLG"
     gene            complement(3020..4408)
                     /gene="lpdA"
                     /locus_tag="VFX87_05475"
     CDS             complement(3020..4408)
                     /gene="lpdA"
                     /locus_tag="VFX87_05475"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX4994395.1"
                     /translation="MGDLVDEVEVAVLGGGPGGYSAAFRCAELGLETVVIDAAQRLGG
                     ACLFEGCIPSKALLHVAAVLSEAERAKEFGVDFGEPRISLDPLRKWKQERVVGKLARG
                     LASTAKAKGVEVIGGRGVFEDSSTLRIELGETLRKVRFKHAIVATGSRPSGLPGLPLQ
                     SERIMDSTSALELPEIPERLLVIGGGYIGLELGTVYATLGSRVTLVEMTDGLLPGVDR
                     DLVRPLQRRVDKIFAAIHLGTKVTALREVGSGIEADLEGRGAERFDRVLVAVGRRAQS
                     DGIGLEATRVRLTERGRFVQVDDCCRTEDPRIYAVGDVTGEPMLAHRAMRQGKVAAEV
                     LAGRPAAFDNVAIPAVVFTDPEIAWCGLSEAQAKDRGLTVKVAKLQWAGSGRSATLGR
                     SDGLTKLVADASSGRVLGVGIVGPGAGELIAEATLAVETAALVEDVAVTIHAHPTLSE
                     SLMEAAEQLLGA"
     gene            complement(4418..5713)
                     /locus_tag="VFX87_05480"
     CDS             complement(4418..5713)
                     /locus_tag="VFX87_05480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX4994396.1"
                     /translation="MPRTFSLPDLGEGLIEAEIVAVLVHEGDVIREDAPLLEVETDKA
                     QVEIPSPMGGRVEKIHVAPGQTVKVGAPLVTFADWVAEPAAAPAPGRSVPAATPAAGA
                     APAAAPARAPAAAPPAPAAARAEGPVAATPATRRLARELSVDLKTVRGTGAGGRVLDE
                     DVRAAAAAPQGTAAPAGPAKPLASVGREAPPLPRFEQWGPVERAPLSHLRRTIAERMT
                     LSATLVPHVTHFDRADITDLDAIITRNLEAARAKGVTLTLTSFLLKATALALLRHPTF
                     NASLDPGAGEVVLKRYYSLGVAVATERGLIVPVLREIDRKPVLEIARDLAALARRVRD
                     GKATLDDLRGGTFTITNIGVLGGTAAIPIINYPEVAILAVARARQEAVVRLGQIVPRL
                     MLPLTLTFDHRVADGADGARFATEIVQLLEAPERLLGEW"
     gene            complement(5713..6678)
                     /locus_tag="VFX87_05485"
     CDS             complement(5713..6678)
                     /locus_tag="VFX87_05485"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007543853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX4994397.1"
                     /translation="MVKALNLALLQEMERDPDVLVIGEDVGVDGGVFRVTEDLHRKFG
                     GTRVMDSPLAEAAIIGASVGMALYGLKPVCEIQFSGFAFQCFHQIENHVARYRMRTQG
                     RFGCQMVLRMPFGGGVRALEHHSESEEQFYAHIPGVKMVIPSGPRNARALLAAAIRDP
                     DPVIFFEAKALYHAAKEDVPDEIETMEIGRAHVDRAGKDLTLVAYGAMVRVAREAADR
                     LHEEDGAEIEILDLLTISPLDREALTASVRKTGRAVVVHEAPKSFGAGAEIAASIMEG
                     AFLSLQAPIRRVTGHDVPFVGFARERANLPDVARVVAACRETISF"
BASE COUNT          892 a         2402 c         2441 g          982 t
ORIGIN      
        1 gccgggcgcc gcgagggcgg cctccgcgag ggtcgccagg tcatcgtcgg tctccgcgtc
       61 ggcggccacg agggcgccgc ggtggcgctc gagcagatgg gcgaccttct ccgcgcccgc
      121 gcggacgtcg tcgagcccga gggtgaccac cggcgcgccg cccgcgagca gggccgcgac
      181 gtcggaggtg gcggcgcgga acgcggggtc gcgggtgatc gcggattcgt gaacgggaac
      241 cgagtcgacc aggagtcggc cacgacggac gacgcggtgc tgagccggga aggcgggaca
      301 gacgagcgcg ccggtgaagg gcggcccata ctcgagcagc gcggtcagct cggcgccgac
      361 ggcgccgcgc agggtggagt cgatcttctt gaacacgtgg cccgcggcga gccgcccccc
      421 cagctgtcgc gcggcgtggt ggatcgcggc ggccgcctcc cggtacggca gggcgcggct
      481 ctcggtgtcg atcgtgatca cgggcatgat ggtggaggag tcggccgcgg tgagcgcggg
      541 cggcgcgagg acgccgaccg ggccggggcc cgcgaagagg cagcccgtat cgcaggcgcc
      601 ggtcaggtca tcggcgatga tcgtgaccac ctgggatgcc gttgccgcca tgagtggata
      661 accgtaccac agaacggccc cttcacggac cgggcccggt gcggagaggc cgcgcccccg
      721 gggtgtatac tttgcccgtg aaaagccgcg tcgtcgtggc gctcctcgcc cttgccgcgg
      781 tgctggcgcc gggcggggcc gcgggagagg atccggtggc cgctctctcc ctgatccgcc
      841 ccaagcccgc caaggatgcg cccgacttcg agctccgcac gcccgacgat cgcgtcatcc
      901 gcctcgcgga cctcaagggc aaggtggtgt tcctgaactt ctgggcgacc tggtgcgagc
      961 cgtgccggga ggagatgccc ggcatggagc gcctgcacct ggcctacaag gaccgcggcc
     1021 tggtggtgct ggcggtctcc ctcgactcgc agggcgcaag cgtggtgatc ccgttcgtca
     1081 agaagttcgg gctcacgttc ctcatcgggc tcgatcccaa gatggcggtg cgcgagcgct
     1141 acggcgtgtg ggcggtgccg tccaccttcc tgatcgaccg caagggcaag cgcgtgctgt
     1201 tcgcgaacgg ggcgcgcgag tggaacggca aggcggccca cgcggtcatc gagtcgctgc
     1261 tcaagtgagc gagtcgctcg gggtcctcgt cgcgttctcg gccggcctct tctccttcct
     1321 gtccccgtgc gtgctgccgc tcttcccgtc gtatctctcg ttcatcaccg ggatgtcggt
     1381 ggaccggctc acccacgagg tgaccgcggg cgcccgcacc cgcgtgctcg tgcactcgct
     1441 ggccttcatc gtgggcttca gcgtggtctt cgtgagcctg ggggcgtcgt tcagcgcggc
     1501 cggccagttc ctgctcgact accgcgagtg gatccgcatc gcgggcggcg cgctcatcat
     1561 cgtcttcggt ctctacatcg cgggtgtcct gcgcatcggg atcttcggcc gcacccagca
     1621 gctccagatc cagagcaagc ctgccgggct gctcggctcc ttcctggtcg gcctgacgtt
     1681 tgcgatcggc tggacgccct gcgtggggcc gatcctgggc tccatcctca ccctggccag
     1741 caatgacaag acggtgggcc agggggtgac gctcctgctc ttctactccg ccggtctcgg
     1801 gctgccgttc ctgctctcct cggtggccct cggcggcttc ctccgcttct tcaagcacta
     1861 ccggccgttc atcccgaccg tggagcgcgc ggcgggggtc ctgctggtgc tcgtgggcat
     1921 cctggtcgcg acgaactact acgtgctgct gaactcctgg gccatcagcc tgacgcccga
     1981 atggctgctc aggcgtctct aacccggaag gaggccacgc atggtgctcc atcggatcgg
     2041 gcccctgtcg gccgcgaagg tggtcgcggt gctctacgcg atcttcggac tcatcatggg
     2101 ggcgttcctc tccgtcgccg cgctcttccg tgctgacggc ggcggcaccg ccccgctgtg
     2161 gggcgtcgcg gcggtggtgt tcttcccggt gctctacggg gtgggcggct tcatcgtgac
     2221 gctcatcacc gcctggccct acaacgtggt cgcgggggcg gtgggcgggg tcgagctcga
     2281 cctgcgctga ccgcggcccc gccgcccagc cgctccgcca cgacgttagc ccagccgtct
     2341 gatccagcgg gcgatctcgt cctgcacccg ctcgaagacc tcctcctgag agatcgggcc
     2401 gcgtttcggg accttgaacg agtgatcgcc gcccgcgacg acctggagcg tcgccgtcgt
     2461 gccgaggggc gcaagcgcgg gccggagctc ctcgggcgtg ccgaaggcgt cgcgcgaccc
     2521 ctgcacgatc agcatcggcc ggcggatccg cgccaggtgc tcggcccgaa gctgctccgg
     2581 tcggccgggc gggtgcaggg ggtagcccag ggccacgagg ccggccagat cctcgacgcc
     2641 ctgggcggcc agatgcgagg cgatgcgccc gcccatggac ttgccgccga tcacgagcgg
     2701 ggccgccgcc agatcggccc ggccgcggat ggccgcgatc acgtcgcgga agcaggcctc
     2761 gagggtgggc gcgcgatcgg gcaggcgccg accctgctcg gtgtagggaa agttgaacgt
     2821 cacggcatgg cagccgcgcc gggcgatccc ctgggcgaac gcgaccatga aggcgctcga
     2881 ctgcggggcc ccggcgccgt gagccaggat cagggtcgcg agggcgggcg acggctcggg
     2941 cggcggatac acgagcgcgc tcacggtgcc cgacgcgacg gcgacgcgaa ccgactcggg
     3001 cagggccggt ccctgaggct caggcaccga gcagctgctc ggccgcctcc atcaagctct
     3061 ccgacagggt cgggtgcgcg tggatggtca cggccacgtc ctcgaccagc gcggcggtct
     3121 ccacggccag ggttgcctcg gcgatcagct cgccggcgcc gggccccacg atgccgacgc
     3181 cgagcacccg cccggacgag gcgtcggcca ccagcttggt gagcccgtcc gaccggccga
     3241 gtgtcgccga gcgtcccgag cccgcccact gcaatttcgc caccttgacc gtgagccccc
     3301 gatccttggc ctgagcttcg ctcagcccgc accaggcgat ctccgggtcg gtgaagacga
     3361 cggccgggat cgccacgttg tcgaacgcgg ccggccggcc ggcgagcacc tccgcggcga
     3421 ccttgccctg tcgcatcgcc cggtgcgcga gcatcggctc gccggtgacg tccccgaccg
     3481 cgtagatccg cggatcctcg gtgcggcagc agtcgtccac ctggacgaag cgcccgcgct
     3541 cggtcagccg cacgcgggtg gcctcaaggc cgatgccgtc gctctgggcg cgccggccca
     3601 ccgccaccag tacccggtcg aagcgctcgg cgccccggcc ttccagatcg gcctcgatcc
     3661 ccgagccaac ctcacggagc gcggtcacct tggtcccgag gtgaatcgcc gcaaaaatct
     3721 tgtccacgcg gcgctggagc ggccggacca gatcacggtc cacgccgggg agcaggccgt
     3781 ccgtcatctc gaccagggtc acccggctgc cgagcgtcgc gtagaccgtg cccagctcga
     3841 ggccgatgta gccgccgccg atcacgagga gccgctcggg gatttcgggc agctcgagcg
     3901 cggatgtgga gtccatgatc cgctcgctct ggagcgggag cccgggcagg ccgctcgggc
     3961 gcgagccggt ggccacgatg gcatgcttga agcggacctt ccggagcgtc tcgccgagct
     4021 cgatccgcag cgtgctggag tcctcgaaga cgccgcgccc gccgatcacc tcgacgccct
     4081 tggccttcgc cgtcgaggcg agcccccgcg ccagcttgcc cacgacccgc tcctgcttcc
     4141 acttccgcag gggatcgagc gagatgcgcg gctcgccgaa gtccaccccg aactccttgg
     4201 cgcgctccgc ctcgctgagg accgcggcca cgtgcaagag cgccttgctg gggatgcagc
     4261 cctcgaagag gcaggccccg cccaggcgct gggccgcgtc gatgaccacg gtctcgaggc
     4321 cgagctcggc gcagcggaag gcggccgagt aaccgcccgg tccgccgccc agcacggcta
     4381 cctccacctc gtccacgaga tcgcccatga ccatggctca ccactccccg aggagccgct
     4441 cgggcgcctc gagtagctgc acgatctcgg tggcgaaacg ggcgccgtcg gcgccgtcgg
     4501 cgacacggtg gtcgaaggtg agggtgagcg gcagcatgag ccgcggcacg atctggccca
     4561 gccgcaccac cgcctcctgg cgggcgcggg ccacggcgag gatggcgacc tccgggtagt
     4621 tgatgatggg gatcgcggcg gtgccgccga gcacgccgat attggtgatg gtgaaggtgc
     4681 cgccgcgcag gtcgtcgagc gtggccttgc cgtcgcggac gcgccgggcc agggcggcga
     4741 gatcccgcgc gatctccagc accggcttgc gatcgatctc gcggagcacc ggcacgatga
     4801 ggccacgctc ggtcgccacc gccaccccga ggctgtagta gcgcttcagg accacctccc
     4861 ccgcgccggg atcgaggctc gcgttgaacg tgggatggcg cagcagggcg agtgccgtcg
     4921 ccttcagcag gaacgaggtc agggtgagcg tcacgccctt ggcgcgggcc gcctcgaggt
     4981 tccgggtgat gatcgcgtcc aggtccgtga tgtcggcgcg gtcgaagtgg gtgacgtggg
     5041 ggaccagggt ggccgagagc gtcatgcgct cggcgatggt ccggcgcagg tgggagagcg
     5101 gcgcgcgctc caccggcccc cactgctcga agcgcggcag cggaggagcc tcgcggccca
     5161 cgctggccag tggcttggcc gggccggccg gcgcggcggt gccctgcggg gcggccgcgg
     5221 cggcgcgcac gtcctcgtcc agcacacggc cgcccgcgcc ggtgccgcgc acggtcttga
     5281 ggtccacgct cagctcgcgg gccagccgcc gcgtggccgg ggtcgcggcc accgggcctt
     5341 cagcgcgagc ggcagcggga gccggcgggg cggcggcggg tgcccgcgcg ggagccgccg
     5401 ccggggccgc gccggcggca ggcgtggcgg ccggtacgct ccggcccggc gccggcgcgg
     5461 cggctggctc cgccacccaa tcggcgaacg tgacgagggg cgcgccgacc ttgacggtct
     5521 ggccgggcgc gacgtggatc ttctccacgc gcccgcccat gggcgagggg atctcgacct
     5581 gtgccttgtc ggtctcgact tccaggagcg gcgcgtcttc gcggatgacg tcgccctcgt
     5641 gcacgaggac cgcgacgatc tcggcctcga tcaagccttc tcctaggtct gggaggctaa
     5701 atgtcctcgg cattagaagg aaatggtctc gcggcaggcg gcgacgaccc gggccacgtc
     5761 gggcaggttc gcgcgctcgc gggcgaagcc cacgaagggc acgtcgtggc ccgtcacgcg
     5821 ccggatcggc gcctggagcg acaggaaggc gccctccatg atgctggccg cgatctcggc
     5881 gccggcgccg aagctcttcg gtgcctcgtg gaccacgacc gcgcggcccg tcttccgcac
     5941 cgaggcggtc agcgcctcgc gatccagcgg cgagatcgtg agcaggtcca ggatctcgat
     6001 ctccgcgccg tcctcctcgt ggaggcgatc cgccgcctcc cgggccaccc ggaccatggc
     6061 cccgtaggcg accagggtga ggtccttgcc ggcccggtcc acgtgggccc ggccgatctc
     6121 catggtctcg atctcgtccg gcacgtcctc cttggccgcg tggtagagcg ccttggcctc
     6181 gaagaagatc accgggtcgg ggtcgcggat cgcggcggcc agcaaggccc gggcattgcg
     6241 ggggcccgag gggatcacca tcttgacgcc ggggatatgg gcgtagaact gctcctcact
     6301 ctccgagtga tgctcgagcg cccgcacgcc gccgccgaag ggcatccgca gcaccatctg
     6361 gcagccgaag cgcccctggg tgcgcatgcg gtatcgcgcg acgtggttct cgatctgatg
     6421 aaagcactgg aaggcgaagc cggagaactg gatttcgcag accggcttga gcccgtagag
     6481 ggccatgccc accgaggcgc cgatgatggc ggcctccgcg agcgggctgt ccatcacgcg
     6541 ggtgccgccg aacttgcggt ggaggtcctc ggtgacccgg aagacgccgc cgtccacgcc
     6601 cacgtcctcg ccaatcacga gcacgtcggg atcgcgctcc atctcctgga gcagcgccaa
     6661 gttcagcgcc ttcaccatgt tcagcttggc cacgagcgcc tctgtcctct catgggc
//
LOCUS       DASUFA010000154         4991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1404474, whole genome shotgun sequence.
ACCESSION   DASUFA010000154 DASUFA010000000
VERSION     DASUFA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404474"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFX87_05490"
     CDS             <1..736
                     /locus_tag="VFX87_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4994398.1"
                     /translation="RLLDETGRPFPEPERARESLAFLRGLIESGVSPAWVTAADEELT
                     RRPFGDGRAIFLRSWPYALDLFELPDSRVRGKVGVAPIPRLHHGPVGAASTGGAHLAV
                     SARTRHRDLAVELARFLTSEAAQRAMTEGAALRPSRPALYRDAALIARDPSLPALHGL
                     MLRGHPRPITPYYLMLSTTLQPEFSAVLVGRKSPDHAMRDATAQVRHLLRSVEPVAAT
                     PAPTPVAGPPVAGPRVPETSVARTPR"
     gene            733..1674
                     /locus_tag="VFX87_05495"
     CDS             733..1674
                     /locus_tag="VFX87_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX4994399.1"
                     /translation="MNPQGEEAKPQRELIRAPGLRKSSARRDAADRRLAWMLLSPAMA
                     VLAALTVYPALWVLWLALQYRIPVFGISRWAGLEHFAFLAVDTRFWNAVRVTGVFTVA
                     SVALEGALGLAIAVALRGQRGGRRTALALLLLAWALPSVVTAKMFEWLYHPAGGLVNF
                     LLGGRAVNWLGDPDLALPSLILADVWRATPFVALLCYARLLTIPPDLYEAAQVDGAAG
                     LQAFARITWPLVRPILLVTMLFRTLDALRAFDIMFVLTGGGPAGSTESLTVYAYRALF
                     QTLQIGFGSAIGVVVFALVMVVAALYLRLIGWQERTA"
     gene            1671..2486
                     /locus_tag="VFX87_05500"
     CDS             1671..2486
                     /locus_tag="VFX87_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4994400.1"
                     /translation="MRTGRLRDGVVLLALALYAAPFFWQALTSLRPDAELLPLTRFLP
                     SHLTWAHYDVVFRQSLMPRALVNSLGIALLSTLLAVGLGALAAYALARLPVRGRGVIL
                     LAMIAATAFPQIATVSPLYLLMRALGLRDTWLALILANGSFALPLVIWLLTGFIREIP
                     RALEEAAAVDGADRLAILRWVVLPLVAPGMASAALLAFLFSWNEFLFAYSFTATEASR
                     TLPVALALFPGVFEVPWGDIAAASLLASLPPILIVVAFQRYLVRGLLAGAVRE"
     gene            complement(2506..3003)
                     /locus_tag="VFX87_05505"
     CDS             complement(2506..3003)
                     /locus_tag="VFX87_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX4994401.1"
                     /translation="MIAPDEIEQRVRQVLDGLGLAYEMVEIDPQYADTAAFCAQYGYP
                     LEQSANTIIVGSKKEPKRYCACVVRATRRVDVNHTVRKLLGVSRLSFADPEETRALTG
                     MMLGGVTVFALPADLPIYVDEGLMPLPWIILGGGSRSLKVKISPEVFRVLPGAAVTPG
                     LVTEP"
     gene            complement(3048..3974)
                     /locus_tag="VFX87_05510"
     CDS             complement(3048..3974)
                     /locus_tag="VFX87_05510"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX4994402.1"
                     /translation="MKRAMVWLIAGAWSVTAALTAAAGGATQAHTLEVGGLTRSYLLH
                     VPPTLPAGPAPLVLVFHGGGGNGPGIERLTRFSELADREGVLIAYPEGVGRNWYDGRE
                     FSSGSRAHRERIDDVGFVAALLDVVARAYAVDPRRIYATGISNGAVFSHYLAAHLSPR
                     IAAIAPVVGGIADPPDAWLRPERPVSVLILQGTTDPLVPYRGGAIAFGRGRIIDTEEA
                     ARRWARLDGAAPEPRPTALAAPGKDHCGGLRLTHSGGRDGSEVVLVRLDGGGHTWPGG
                     PQYLPEGLIGRVCRDFSATAVIWEFFRTHPKP"
     gene            4058..4852
                     /locus_tag="VFX87_05515"
     CDS             4058..4852
                     /locus_tag="VFX87_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX4994403.1"
                     /translation="MSTDLLEQVKDGVAVLTLNRPDRLNAMSRPMLDALLEALPRLAE
                     DPGVGVVVLTGAGRGFCAGGDVKAMAEGNELGGQTMEERAQALRSRMETSRWLHEMPK
                     PTIAMMRGPAAGAGLSLAMACDMRVASDTVKLGTAFARVGYSGDFGGSYYLTQLVGTA
                     KARELYFTADLLDAQQALGLGLVNRVVADARLEEETMALASRLARGPRVAYRYMKRNM
                     NAAESASLKDMLDLEAWNHTRTGMTEDHREAARAFVEKREPQFKGR"
BASE COUNT          622 a         1835 c         1788 g          746 t
ORIGIN      
        1 gcggctcctc gacgagacgg ggcggccgtt ccccgagccc gagcgcgcgc gcgagtcgct
       61 cgccttcctg cgcggcctca tcgagagcgg cgtgagcccg gcctgggtca ccgcggcgga
      121 cgaggagctg acgcgccggc cgttcggcga cggccgggcc atcttcctgc ggagctggcc
      181 ctacgcgctg gacctcttcg agctgccgga ctcgcgcgtg cgcggcaagg tgggcgtcgc
      241 gccgatcccg cgtctgcacc acggccccgt cggcgcggcc tccaccggcg gcgcgcacct
      301 ggcggtgagc gcgcgcacgc gccaccgcga cctggcggtg gagctggcgc gctttctcac
      361 cagcgaggcc gcccagcggg cgatgacgga gggcgccgcg cttcgcccct cacgccccgc
      421 cctctaccgc gacgccgcgc tgatcgcccg ggacccgagc ctgcccgcgc tccacgggct
      481 gatgctccgc ggccacccgc gtccgatcac gccctactac ctgatgctct ccaccacgct
      541 tcagcccgag ttctccgcgg tcctggtcgg ccgcaagtcc cccgaccacg cgatgcgcga
      601 cgccaccgcc caggtccggc acctcctgcg ctcggtcgag ccagttgccg ccacgccggc
      661 cccgacgccg gttgccgggc cgccagttgc cggtccgcgg gttccggaga catcggttgc
      721 gaggacgccg cgatgaaccc gcagggcgaa gaggccaaac cgcagcgcga gctcattcgc
      781 gcgcccgggc ttagaaaaag cagcgcccga cgcgacgccg ccgaccgccg gctggcgtgg
      841 atgctcctgt ccccggcgat ggccgtcctc gccgccctca cggtgtatcc cgcgctctgg
      901 gtcctgtggc tcgcgctcca gtaccgcatc ccggtgttcg gtatctcgcg ctgggccgga
      961 ctcgagcact tcgcgtttct cgcggtggac acgcgcttct ggaacgcggt gagggtcacc
     1021 ggcgtgttca cggtggcctc ggtcgcgctg gagggcgcgc tgggcctggc catcgcggtg
     1081 gcgctgcggg ggcagcgcgg cggccggcgc accgccctgg cgctgctgct cctggcctgg
     1141 gcgctcccgt cggtggtgac ggccaagatg ttcgagtggc tctatcaccc ggcgggcggg
     1201 ctcgtgaact tcctgctggg ggggcgggcc gtcaactggc tcggcgatcc ggatctggcc
     1261 ctgccgtcgc tcatcctggc cgacgtgtgg cgcgcgacgc cgttcgtggc gctgctctgc
     1321 tacgcgcgcc tcctgacgat cccgccggat ctctacgagg ccgcccaggt ggacggcgcg
     1381 gcagggctcc aggccttcgc ccgcatcacc tggccgctcg tgcggccgat cctcctcgtg
     1441 acgatgctct tccgcacgct cgacgcgctc cgcgccttcg acatcatgtt cgtgctgacg
     1501 ggcggggggc cggcgggcag caccgagtcg ctcacggtct acgcctatcg cgccctgttc
     1561 cagacgctgc agatcggctt cggctccgcc atcggcgtcg tggtgttcgc gctcgtgatg
     1621 gtcgtcgccg ccctgtacct gcgcctgatc ggctggcagg agcgaacggc gtgaggacgg
     1681 gacgcctgcg cgacggcgtg gtgctgctcg ccctggcgct ctacgccgcg ccgttcttct
     1741 ggcaggcgct cacctcgctg cggcccgacg ccgagctgct gcccctgacc cggttcctgc
     1801 cgagccatct cacgtgggcg cactacgacg tggtcttccg ccagagcctg atgccgcgcg
     1861 ccctggtcaa tagcctgggc atcgcgctcc tcagcacgct gctcgcggtg gggctcggag
     1921 cgctcgccgc ctacgcgctg gcccggctgc cggtgcgggg gcgcggcgtg atcctgctcg
     1981 cgatgatcgc ggcaaccgcg tttccccaga tcgccaccgt gagcccgctc tacctgctga
     2041 tgcgcgcgct gggcctgcgc gacacgtggc tggcgctcat cctggcgaac ggctcgttcg
     2101 cgctgccgct ggtgatctgg ctgctcaccg gcttcatccg cgagatcccg cgcgcgctcg
     2161 aggaggcggc ggcggtggac ggcgccgacc gcctggcgat tctcagatgg gttgttctac
     2221 cgctggtggc gccgggcatg gcttcggcgg cgctgctcgc gttcctgttc tcgtggaacg
     2281 agttcctgtt cgcctacagc ttcacggcga ccgaggccag ccgcaccctg ccggtcgcgc
     2341 tggccctctt ccccggcgtc ttcgaggtgc cctgggggga tatcgcggcc gcctcgctgc
     2401 tggcgagctt gccgccgatc ctcatcgtgg tggcctttca gcgctacctg gtccgcggcc
     2461 tcctggccgg cgccgtgcgc gagtgacccc tctccccgga ggtgcctacg gttccgtcac
     2521 caaccccggc gtgaccgcgg cgcccggcag cacgcggaac acctcgggcg agatcttcac
     2581 cttcagcgag cggctcccgc cgcccaggat gatccagggc aggggcatca gcccctcgtc
     2641 cacgtagatg ggcagatcgg cgggcagggc gaacacggtg acgcccccca gcatcatgcc
     2701 ggtgagcgcg cgggtctcct ccggatccgc gaaggagagc cgggagacgc ccagcagctt
     2761 ccgcaccgtg tgattgacgt ccacccgccg ggtggcgcgg accacgcagg cgcagtaccg
     2821 cttcggctcc ttcttggagc cgacgatgat ggtgttggcc gactgctcca gcgggtagcc
     2881 gtactgcgcg cagaacgcgg cggtgtcggc gtactgcggg tcgatctcga ccatctcgta
     2941 ggccaggccg agcccgtcga ggacctgtcg tacccgctgc tcgatctcgt ccggggcgat
     3001 caccgggagc tgatcctccg tgtgtggatt cgcgcgagca taccgcgcta cggcttcgga
     3061 tgggtgcgga agaactccca gatgaccgcg gtggcgctga agtcgcggca gacgcggccg
     3121 atgagccctt ccggcaggta ctgggggcca ccgggccagg tgtggccgcc gccgtcgagt
     3181 cgcaccagca ccacctcgct gccgtcgcgg ccgcccgagt gcgtgagccg gaggcccccg
     3241 cagtggtcct tgccgggtgc ggctagcgcg gtcggccgcg gctcgggcgc ggccccatcg
     3301 agccgggccc agcggcgcgc cgcctcttcc gtgtcgatga tccggccacg cccgaaggcg
     3361 atggcgccgc cccggtacgg caccagcgga tccgtcgtgc cctgcaggat gagcaccgag
     3421 accggtcgct cgggccgcag ccaggcgtcg ggcgggtccg cgatcccgcc caccaccggc
     3481 gcgatggccg cgatgcgcgg ggagaggtgc gcggccaggt agtgggagaa gacggcgccg
     3541 ttggagatgc cggtggcgta gatccgccgc gggtccaccg cgtacgcgcg tgcgacgacg
     3601 tccagcagcg cggccacgaa gcccacatcg tcgatccgct cgcggtgggc ccgcgagccg
     3661 ctgctgaatt cgcgcccgtc gtaccagttc cggccgacgc cctcgggata cgcgatcaag
     3721 acgccttcgc gatcggccag ctcgctgaag cgggtgaggc gctcgatgcc ggggccattg
     3781 ccgcccccgc cgtgaaagac gagcacgagc ggcgcggggc cggcgggcag ggtcggcggc
     3841 acgtgcagca ggtagctccg ggtgaggccg ccgacctcga gggtgtgcgc ctgggtggcc
     3901 ccaccggccg ccgcggtcag cgccgcggtc acgctccacg cccccgcgat cagccagacc
     3961 atcgcgcgtt tcatcgtcgg gccttcattg tacaatcacc cgtccacgct tccgattcct
     4021 cacgacgatc cggcggcgcc gccggaggag acccacgatg agcaccgatc tgctcgagca
     4081 ggtgaaggac ggagtggccg tgctgaccct caaccgcccc gatcggctga acgcgatgtc
     4141 gcggccgatg ctggacgccc tgctggaagc tctgccgcgg ctggccgagg acccgggggt
     4201 cggggtggtt gtgctcaccg gggccggccg cggcttctgc gcgggcggcg acgtcaaggc
     4261 catggcggag ggcaacgagc tgggcggcca gaccatggag gagcgtgcgc aggctctgcg
     4321 ctcgcggatg gagacctcgc gctggctcca cgagatgccc aagcccacca tcgcgatgat
     4381 gcgggggccg gccgcgggcg ccgggctgtc gctggcgatg gcctgcgaca tgcgcgtcgc
     4441 gagcgacacg gtgaagctcg gtaccgcgtt cgcgcgcgtc ggctactccg gcgacttcgg
     4501 cggcagctac tacctgaccc agctcgtggg caccgccaag gcgcgcgagc tctacttcac
     4561 cgcggatctg ctcgacgccc agcaggcgct cggcctgggc ctggtgaacc gcgtggtggc
     4621 cgacgcgcgc ctcgaggagg agacgatggc gctggcctca cgcctggccc gcgggccgcg
     4681 cgtggcctat cgctacatga agcggaacat gaacgcggcg gagagcgcct ccctcaagga
     4741 catgctcgat ctcgaggcgt ggaaccacac gcggaccggc atgaccgagg accaccggga
     4801 ggccgcgcgc gccttcgtgg agaagcgcga gccccagttc aaggggcgct agaactcggg
     4861 acccctcacc ccgaccctgt ccccagaggg gagagggagt cttgaccctc gccctcggag
     4921 ggggagaggg cggggtgagg gggcgcgttt ccgacgcgcc acgcgaggta caacacgccc
     4981 gcggtcagcc c
//
LOCUS       DASUFA010000155        12320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_608730, whole genome shotgun sequence.
ACCESSION   DASUFA010000155 DASUFA010000000
VERSION     DASUFA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608730"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFX87_05520"
     CDS             complement(<1..254)
                     /locus_tag="VFX87_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4994404.1"
                     /translation="MSRFIDLSVTVDENTFSPPSTNMKLEVTPHRRGPGFWQVSSVHQ
                     SLHTGAHIDSPLHVFKNGITTAEITLDQVMGEALVVDLSGV"
     gene            complement(251..1681)
                     /locus_tag="VFX87_05525"
     CDS             complement(251..1681)
                     /locus_tag="VFX87_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994405.1"
                     /translation="MSAWRSALLMGLLALVPTPAGAAEQHDMRLVGAHDLQARSAYQP
                     VIHQQGGRFIAYVGHHGGLRMNPLTGQMEQNGTSIVDVTDPKRPRYLHHIPGAPGEAE
                     AGGAAMARVCDGKALPRGAREKTYLLRTFGNLAHEIWDVTDPAKPARLTTVLDGLKST
                     HKNWWECDSGIAYLVSDGAPRGWRTNRMTTIYDLSDPAQPRFIRDFGLPGQEPGAPGP
                     APEGVHGPIAYRNRVYFAYGTGAKGVLQIVDRDKLLAGDPQARERFAPTVENLLYPQI
                     GRLDMSPAWGGHTSFPVLGVRVPHWSPGVPGRTRDFVLLVSESLRNECQEVRQLSFMV
                     DVTTERTPYSVATFEVPDPTGDFCRRGGRFGPHSSHESFTPVFYGRLVFLAYFNAGVR
                     AVDIRDPFHPVEAAYFIPEVTARTDKRCVEVAGTPRCKTAIQTNNVEVDDRGYIYLAD
                     RADTGLHIVELTGPARAIANLPRMTP"
     gene            1797..2684
                     /locus_tag="VFX87_05530"
     CDS             1797..2684
                     /locus_tag="VFX87_05530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994406.1"
                     /translation="MATIGLVGVGLLGAAVAGRLLEAGHRVVGFDTAPDRVRALLNMG
                     GEAATSARAVALAGDAVCTLLPTLATVEAAVLGPDGVAAARKAGQVVIQMSTISPTLS
                     ARLAAESRTRGLDFLDCPVSGTSGVVARGEGVFFVGGDRAVFERWRPLLEAMLPRAVY
                     IGPAGQAMALKLVANLLVALHSAAAAEALHLARQAGLDPALALDVLAGSAAGSRMLEV
                     RGPLMVRGEYPAQMKLDLFMKDLHLIQEAAAIAGTALPLTDTAERLYAAAAETGHGDE
                     DLAVVLTALAALSPRRREA"
     gene            2706..4253
                     /locus_tag="VFX87_05535"
     CDS             2706..4253
                     /locus_tag="VFX87_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4994407.1"
                     /translation="MAPARTPEFTLRWITTVGNLLGAIVAFLYFRVVDHAASRLPPVR
                     WIDVVVSIFLFALLVWSGVRLSGPWTRPLNRVAELGTLPPAEADLLRRRALMFPYFLA
                     GLSFLGWTLAGLIWGVFWPLLAGVFTPLHSLRLLFGTTVIAGGTTVAFIFFASEYHWR
                     RRLPEFFPAGDLSAVKRVPRLTVRVRLLAILLLIGLVPLAMLGMLAYTRAVALVGTDP
                     IAAAEIRDGLRVTILFLVGAGVVVAVGLSVFVAHSVAAPLRDVEAAMAEVERGRLDGH
                     VPVVSTDEIGAVAEGFNRMLRGLREREMVKETFGKYVTPEIRDAILAGRVRGEGELVE
                     ATMLFADLRDFTPWVEATEPRQVVRDLNEYFTEMDQAIRAHGGLVLQFIGDEIEAVFG
                     APIASRDHAGRAVHAAIEMRRRLQAWNARREAAGRPPLRNGIGIHTGTVLAGNIGGAE
                     RLSYALVGDPVNLASRIQGLTKDFKADILISDATRGRLDGSVVVEELPAVRVKGRVEE
                     VNVYKVV"
     gene            complement(4361..5131)
                     /locus_tag="VFX87_05540"
     CDS             complement(4361..5131)
                     /locus_tag="VFX87_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994408.1"
                     /translation="MRQLYDTIGVGYRALRRPDPRIAAAIVSALGPAASVLNVGAGAG
                     SYEPADRRVVAVEPSREMIRQRPAGAAPVVQASATALPFRAGAFDAALAVLTVHHWPD
                     RARGLAELRRVARDRVVILTWEPDAAGFWLVEDYFPELVAIDRAIFPTRDELEHSLGP
                     VELRPLPIPHDCVDGFLGAYWRRPRAYLDPAVRGAISTFSKLGDVAAGLARLRNDLED
                     GTWSRRHGPLLERAELDLGYRLVVAPTENPLPSGERAG"
     gene            complement(5203..6264)
                     /locus_tag="VFX87_05545"
     CDS             complement(5203..6264)
                     /locus_tag="VFX87_05545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994409.1"
                     /translation="MRFGTYYFLQAPPGHRHADIIARELDQMAWSEELGFDEVWLTEH
                     HFIDYGLSVDPATLACAVASRTSRIRIGLAAAILPFHDPIRLAEQMALVDIISKGRLD
                     VGVGRGNRPSEFRGYRIPQVESRERFDEAVQIMVRAWTEERFSYDGRFFQLDDVRVIP
                     KPLQQPHPPVYQVCVSPDSIEGTALRGWPMLNSILFGGVEQIVASRDRYVATLQKAGR
                     SPADIAALLSRWGVSRQIYVAPTDAQALAEAKDAEMWYQESFRKFVIPERIEDSHPTL
                     QPGFRAMADRLSKITWEGLVAETLAFGSPDTVARHIEQMRQMGVGQVLCWMNFGGLPE
                     DKVRRSMELFAREVMPRFR"
     gene            complement(6264..6959)
                     /locus_tag="VFX87_05550"
     CDS             complement(6264..6959)
                     /locus_tag="VFX87_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994410.1"
                     /translation="MALEVEGVHTYYGESHVLQGISLRVEAGEVLAILGRNGMGKTTL
                     IRTIVGFTPPREGRVRYKDTEISRLPPFRMVELGMGLVPQGRRVFPSLSVRENLEVAR
                     RGAGRWSLEQVYALFPRLRERAANRANKLSGGEQQMLAIGRALMSNPDLLLMDEPTEG
                     LAPLLVREVGRVIAELKRSGLSILLVEQNLALALSVADRVHVLSRGRIVHTGTSAELM
                     TNDDVKTRYLGVA"
     gene            complement(6949..7692)
                     /locus_tag="VFX87_05555"
     CDS             complement(6949..7692)
                     /locus_tag="VFX87_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994411.1"
                     /translation="MTDHALHLDAVCRAFGGLQAVDGVTLAVRPGERRALIGPNGAGK
                     TTLFNLIAGAMPLSSGRITLFGRDVTHAPAHQRAALGLARTFQITNLFPDLSVLENCL
                     LAVQAHSPVRFSMLRPVGRYPALLERARVALQRLELEKLGGAVVRNLSHGEQRQLEIA
                     LALAGQPRVLLLDEPTAGLSPAESRLMAGLLRRLDPEITVLMIEHDMDIALELSEMVT
                     VLHYGRVIADGPREQVKADPQVKEIYLGA"
     gene            complement(7689..8612)
                     /locus_tag="VFX87_05560"
     CDS             complement(7689..8612)
                     /locus_tag="VFX87_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994412.1"
                     /translation="MIGRRALLAAVLIAVLAVAGPWLPAYPLTVLTQALIVAILAMSL
                     DVLLGYTGLSSLGHAAYFGVGAYAVAIMTTEHQMGLLACLAAGVALATLTAAVFGLLA
                     IRATGTYFLMITLALSMVIWGLAYRWVSLTKGDNGIAGIPRPTLLGIELIAAVPFYYF
                     CLACAVMAWVLMGLLVISPFGLGLRGIRGSEGRMQALGYNVWLHKYIAFVLSGVFGGF
                     SGTLWAYYNGFVSPNDVQLVTSVEALLMVALGGSGTLIGPALGAGIIVFLKNFVSVYT
                     KRWLLILGGVYIGVILFAPAGIVGALRGRRR"
     gene            complement(8609..9469)
                     /locus_tag="VFX87_05565"
     CDS             complement(8609..9469)
                     /locus_tag="VFX87_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994413.1"
                     /translation="MSAEFWIAQSFNGLSYGALLFLLASGLSLIFGVMRIVNLAHGSY
                     FMLGGYVGLSVALYTRSFALACLAGAVAIALVGMGMERFFLRRLRGEVLGQVLMTIGF
                     ALIFQDLALLIWGGDPYTVPIPKMLTGTVRVGGAVFPIFRIFIVAVAVVVGLALWLAM
                     DRTRVGAMIRAAVDDAEMAQGVGINVPRVSLAVFALGAGLAALGGVIGGGFLGVYPGA
                     DFEVLPYAFVVVIVGGLGSLPGAVAGSLLVGLLDNFGKALFPELSYFTLFAPMAIILA
                     IRPTGLFGRG"
     gene            complement(9485..10489)
                     /locus_tag="VFX87_05570"
     CDS             complement(9485..10489)
                     /locus_tag="VFX87_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994414.1"
                     /translation="MAAPLGIDVHAHFFPERFLRAIEDGGAPSGGRIDRSSPVGPVVD
                     VKGARTAPLEARYYDLPKRVASMNRQGVAVQALSLTAPMVYWAQGELGRRLAREWNDG
                     ASAAHTAYPDRFVVCATLPMQDTTLALAELDRAARLPGVRAVYMGTNVNGRNLSDPEF
                     FPVFERCQALGLPVGLHPFNVNGAERLKPFYLTNLLGNPYDTGIAAAHLIFGGVLDRL
                     PKLQVILPHAGGTFPLVWGRLQRGQKVRPEANKAAKKPVRAYLRRFTYDTISHDAGAL
                     RYLIDTVGADRVMLGTDFCFDMGYERPLDIIRDKAVKLSRADQAKVVRENAARLLRVR
                     "
     gene            complement(10545..10757)
                     /locus_tag="VFX87_05575"
     CDS             complement(10545..10757)
                     /locus_tag="VFX87_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994415.1"
                     /translation="MRQTGEILRSAARGQSEAYDRANKGFSYYLRGVEVLEHSPSGAR
                     GNFDRDYADAVVKADPNKFRLVLPSQ"
     gene            complement(10820..>12320)
                     /locus_tag="VFX87_05580"
     CDS             complement(10820..>12320)
                     /locus_tag="VFX87_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994416.1"
                     /translation="PDTPDVPDLRWSALDSDPRAPVAIARLTDTDRYAGGNPGLTMAA
                     LTGLAEYTNANFFSEDRIFTENDVDPLKRFPYPNRSSVMEQDFDVSVGGSTAKRRYFV
                     KTADGATGYRLATVGLLRDYQQRFNLDWTRFRQGPALDEAVYRDYAERLVPRAIAYST
                     ALLDYFFRGLVIAFGNDLSFGFANGTDEAMDGTFTLYYDDVSDVRRPVPGAQWTRALA
                     PSGLVEDLKVSAPSSPMPKEPGRYMLVFRGSLGSERDAVVGRQVVIEPMMVVRLIKRS
                     DGTPHRGAAVHAIDVQSGAVLGSGLTDQDGRAELAWRPGKTVLFIPAVNLFPMYWAGE
                     STFSSSIEGGRIVQTTDLDAQRQVTIPVPLILASWPERIEPCTEVPLFAHQPRGFFQQ
                     SVSLGDGLADFVFVTYGVNLVTFIRGDNGQETELCGGTSTGCIDPRAGFVAEDVNRVG
                     QVVGRLVRDMFARHIRQVVHEEGGEPVGQPICDTEYSEVEVIPVTVAEE"
BASE COUNT         1839 a         4357 c         4339 g         1785 t
ORIGIN      
        1 accccggaga gatccaccac caaggcctcg cccatcacct ggtccagcgt gatctcggcg
       61 gtggtgatgc cgttcttgaa cacgtgcagc ggcgagtcga tgtgggcgcc ggtgtgcagg
      121 ctctggtgca cgctcgagac ctgccagaag cccgggccgc ggcggtgcgg tgtcacctcg
      181 agcttcatgt tggtcgaggg cgggctgaaa gtgttctcgt ccaccgtcac ggacaggtcg
      241 atgaatcggc tcatggcgtc atcctgggca ggttggcgat ggcgcgggcg ggcccggtca
      301 gctccacgat gtgcagcccg gtgtcggccc ggtccgccag gtagatgtag ccccggtcgt
      361 ccacttccac gttgttggtc tggatcgcgg tcttgcagcg cggggtgccg gccacctcca
      421 cgcagcgctt gtcggtccgg gcggtgacct cgggaatgaa gtaggccgcc tcgaccggat
      481 ggaacggatc gcggatgtcc accgcgcgca cgcccgcgtt gaagtaggcg aggaacacga
      541 ggcggccgta gaagacgggg gtgaacgact cgtggctcga gtgcggcccg aagcgcccgc
      601 cccggcggca gaagtctccg gtcggatcgg gcacctcgaa ggtggccacc gagtagggcg
      661 tccgctcggt ggtgacgtcc accatgaagc tgagctggcg gacctcctgg cactcgttgc
      721 gcagcgactc cgagaccagc agcacgaagt cgcgcgtgcg ccccggcacc ccgggcgacc
      781 agtgcggcac gcgcaccccc agcaccggga agctcgtgtg cccgccccag gccggcgaca
      841 tgtcgaggcg gccgatctgc gggtagagca ggttctccac ggtgggcgcg aacctctcgc
      901 gcgcctgcgg atcgcccgcg agcagcttgt cgcggtccac gatctgcagc acgcccttgg
      961 cgccggtgcc gtaggcgaag tagacgcggt tcctgtaggc gatgggcccg tgcacgccct
     1021 cgggcgcggg gccgggcgcg cccggctcct ggccgggcag cccgaagtca cggatgaagc
     1081 gcggctgcgc cgggtcgctc aggtcgtaga tcgtggtcat ccggttggtg cgccagccgc
     1141 gcggggcccc gtcggagacg aggtaggcga tgccgctgtc gcactcccac cagttcttgt
     1201 gcgtgctctt gagcccgtcc agcaccgtgg tcagccgtgc gggcttcgcc gggtccgtca
     1261 cgtcccagat ctcgtgcgcc aggttgccga acgtacgcag gagataggtc ttctcgcgcg
     1321 ccccgcgcgg cagcgccttg ccgtcgcaga cgcgcgccat ggccgcgccg cccgcctccg
     1381 cctcccccgg cgcgccgggg atgtggtgca ggtacctcgg ccgcttcgga tccgtcacgt
     1441 ccacgatgga cgtgccgttc tgctccatct ggccggtgag cgggttcatc cgcaggccgc
     1501 cgtggtggcc cacgtaggcg atgaagcggc cgccctgctg gtggatcacg ggctggtagg
     1561 cggagcgcgc ctgcagatcg tgggcgccca ccaggcgcat gtcgtgttgc tcggccgccc
     1621 ccgccggcgt cgggacgagc gccagcaggc ccatcaggag ggcgcttcgc cacgcgctca
     1681 cggctcggtc tccgtcgcca tcgacgccgc ctccgctggc ggccccccag cctacccggg
     1741 ggcccgcgga gcggtcaata gcaatccccc ctctccctgt gctatctcct gctcccatgg
     1801 cgacgatcgg cctggtgggc gtgggactgc tgggcgcggc ggtggccggg cgactgctcg
     1861 aggccgggca tcgcgtggtg ggcttcgaca ccgcgcccga ccgcgtgcgc gcgctgctga
     1921 acatgggcgg cgaggcggcc acctcggcgc gcgcggtcgc gctcgccggc gacgcggtgt
     1981 gcacactgct gcccaccctg gccacggtgg aggcagccgt tctcgggccc gacggcgtgg
     2041 cggcggcccg gaaagccggc caggtcgtca tccagatgag cacgatctcg cccacgctga
     2101 gcgcgcgcct cgcggcggag agccggaccc gcgggctcga cttcctcgac tgcccggtca
     2161 gcggcaccag cggcgtggtg gcgcgcggcg agggcgtgtt cttcgtgggg ggcgatcgcg
     2221 cggtgttcga gcgctggcgc ccgctgctcg aggccatgct cccgcgcgca gtctatatcg
     2281 gcccggccgg ccaggccatg gcgctcaagc tcgtggcgaa cctcctcgtg gcgctgcaca
     2341 gcgcggcggc cgccgaggct cttcacctcg cacggcaggc cgggctcgac cccgcgctgg
     2401 ccctcgacgt gctggcgggc agcgcggcgg gctcgcgcat gctcgaagtc cgggggccgc
     2461 tgatggtgcg cggagagtat ccggcgcaga tgaagctcga tctcttcatg aaggacctgc
     2521 acctgatcca ggaggccgcc gccatcgcgg gcaccgccct gccgctcacc gacacggccg
     2581 agcggctcta cgccgcggcg gccgagacgg gccacggcga cgaggatctc gcggtggtgc
     2641 tgaccgcgct ggccgcgctg agtccgcgcc gacgggaggc ctagccggcg gtttacaatc
     2701 gcggcatggc ccctgcccgg actccggagt tcacgctgcg gtggatcacc accgtcggca
     2761 acctcctggg cgcgatcgtc gccttcctct acttccgcgt ggtggatcac gccgcgtcgc
     2821 gcctgccccc ggtccgctgg atcgacgtcg tcgtctcgat cttcctgttc gccctgctgg
     2881 tctggagcgg ggtccggctc agcggcccgt ggacccggcc gctcaatcgc gtcgcggagc
     2941 tgggcacgct gccgcccgcg gaggcggacc tgctgcgccg gcgcgcgctg atgttcccct
     3001 attttctcgc cgggctcagc ttcctcggct ggacgctggc cggcctcatc tggggcgtct
     3061 tctggcctct cctggccggg gtcttcacgc cgcttcattc gctccgtctg ctcttcggca
     3121 ccaccgtgat cgcgggcggc accaccgtcg ccttcatctt cttcgcgagc gagtatcact
     3181 ggcggcggcg cctgcccgag ttcttccccg cgggcgacct gagcgcggtg aagcgcgtgc
     3241 cccggctcac ggtgcgcgtc cggctgctcg ccatcctcct gctgatcggc ctggtgccgc
     3301 tcgcgatgct ggggatgctg gcctacaccc gtgcggtggc cctggtcggc accgacccga
     3361 tcgccgccgc cgagatccgg gacggtctcc gggtgaccat cctgttcctg gtcggggccg
     3421 gcgtcgtcgt cgccgtcggg ctgtccgtct tcgtcgccca cagcgtggcg gcgccgctcc
     3481 gggacgtgga ggccgcgatg gccgaggtgg agcgcgggcg gctcgacggc cacgtgcccg
     3541 tcgtgagcac cgacgagatc ggtgcggtcg ccgagggctt caaccgcatg ctgcgcggcc
     3601 tgcgcgagcg cgagatggtc aaggagacct tcggcaagta cgtgacgccc gagatccgcg
     3661 acgcgatcct cgccgggcgc gtccgcggcg agggcgagct cgtcgaggcc accatgctgt
     3721 tcgcggacct gcgcgacttc acgccgtggg tggaggccac cgagccgcgc caggtcgtgc
     3781 gcgacctcaa cgagtacttc accgagatgg accaggccat ccgcgcgcac ggcggcctcg
     3841 tgctccagtt catcggcgac gagatcgagg cggtgttcgg cgcgcccatc gcctcccgcg
     3901 accacgccgg gcgggcggtg cacgcggcga tcgagatgcg gcggcggctc caggcgtgga
     3961 acgcgcgccg cgaggcggcc ggccggcccc cgctgcgcaa cggcatcggc atccacaccg
     4021 gcaccgtgct cgccggcaac atcggcggcg ccgagcgcct ctcctacgcg ctcgtcggcg
     4081 acccggtgaa cctcgcctcg cgcatccagg ggctcacgaa ggacttcaag gcggacatcc
     4141 tgatcagcga cgccacccgc ggccggctcg acggctcggt cgtggtcgag gagctccccg
     4201 cggtacgcgt caagggccgc gtggaagagg tgaacgtcta caaggtggtg tgagccgccg
     4261 cgacccggca gaagctcgag ttggtccgcg ccgcggccgg tgatcgcttc gattcgggta
     4321 cggcatctcg tatctcacgg tcttcgaccc ctcgggcccc tcaccctgcc ctctcccctg
     4381 aggggagagg gttctcggta ggggcgacca ccaggcgata gccgagatcg agctcggcgc
     4441 gctccagcag cggcccgtgc cgccggctcc acgtgccgtc ctccaaatcg ttccgcagtc
     4501 gagccaggcc cgccgccacg tcgccgagct tgctgaaggt ggagatcgcg ccgcgcaccg
     4561 cgggatccag gtaggcgcgc ggccgccgcc agtaggcgcc gaggaagccg tccacgcagt
     4621 cgtgcgggat cggcagcggg cgcagctcga ccgggcccag gctgtgttcc agctcgtcgc
     4681 gggtcgggaa gatcgcgcgg tcgatcgcca ccagctccgg aaagtagtcc tcgaccagcc
     4741 agaagcccgc cgcgtccggc tcccacgtga ggatcaccac gcggtcgcgg gccacgcggc
     4801 gcagctcggc caggccccgg gcacgatcgg gccagtggtg cactgtgagc acggccagcg
     4861 ccgcgtcgaa cgctccggcg cggaacggca gcgcggtggc cgaggcctgg accaccgggg
     4921 cggcgccggc gggccgctgg cggatcatct cgcgcgaggg ctcgacggcg accacgcggc
     4981 gatccgcggg ctcgtaggag ccggcgcccg cgcccacgtt cagcacggac gccgcgggac
     5041 cgagggcgct gacgatggcg gcggcgatgc gcgggtcagg acggcggagg gcgcggtagc
     5101 cgacgccgat cgtgtcgtag agctggcgca cgttgaggcc tcgatccccc tcaccctgcc
     5161 ctctccccct cgacggggga gaggatcata gggaggaagg gctcatctga agcggggcat
     5221 gacctcgcgc gcgaagagct ccatcgagcg gcgcaccttg tcctcgggca ggccgccgaa
     5281 gttcatccag cacaggacct gccccacgcc catctggcgc atctgctcga tgtggcgcgc
     5341 caccgtgtcc ggcgagccga aggccagcgt ctcggccacg agcccctccc acgtgatctt
     5401 cgaaagccga tcggccatgg cccggaagcc cggctggagc gtgggatggc tgtcctcgat
     5461 gcgctcgggg atgacgaact tccggaacga ctcctggtac cacatctcgg cgtccttggc
     5521 ctcggccagc gcctgcgcgt cggtcggcgc cacgtagatc tggcgcgaga cgccccaccg
     5581 ggagagcagc gccgcgatat cggcggggct acggccggcc ttctggagcg tcgccacgta
     5641 gcggtcgcgg ctggccacga tctgttcgac gccgccgaag aggatggagt tgagcatggg
     5701 ccagccgcgc agcgcggtgc cctcgatgct gtccggggac acgcacacct gatagaccgg
     5761 cgggtgcggc tgctgcaggg gcttcgggat cacgcgcacg tcgtcgagct ggaagaagcg
     5821 gccgtcgtag ctgaagcgct cctcggtcca ggcgcgtacc atgatctgca cggcctcgtc
     5881 gaagcgctcg cggctctcca cctgcgggat gcggtagccg cggaactccg acggccggtt
     5941 gccccgcccg accccgacgt cgagccggcc cttcgagatg atgtccacca gcgccatctg
     6001 ctcggcgagc cggatcggat cgtggaacgg caggatggcg gcggccaggc cgatccggat
     6061 ccgggaggtc cgcgaagcca ccgcgcaggc gagggtggcc ggatccaccg agaggccgta
     6121 gtcgatgaag tggtgctcgg tgagccagac ctcgtcgaag cccagctcct ccgaccacgc
     6181 catctggtcg agctcgcgcg cgatgatgtc ggcgtgccgg tggcccggcg gcgcctgcag
     6241 gaagtagtag gtcccgaagc gcatcaggcc acccccaggt aacgtgtctt cacgtcgtcg
     6301 ttggtcatca gctccgcgga cgtcccggtg tggacgatcc gcccgcggct cagcacgtgc
     6361 acccggtcgg ccaccgacag ggccagcgcc aggttctgct ccaccagcag gatggacagc
     6421 ccgctccgct tgagctccgc gatcacccgt cccacctcgc gcaccagcaa cggcgccagg
     6481 ccttcggtcg gctcgtccat cagcagcagg tcggggttcg acatgagcgc ccgcccgatc
     6541 gccagcatct gctgctcgcc gcccgagagc ttgttggcgc gattggccgc ccgctcgcgc
     6601 agccgcggaa acagagcata cacctgctcg aggctccatc gcccggcgcc gcggcgcgcc
     6661 acctcgaggt tctcgcggac gctgagcgac gggaagaccc ggcggccctg cggcaccagg
     6721 cccatgccca gctcgaccat gcggaacggg ggcaggcggc tgatctcggt gtccttgtag
     6781 cgcacgcggc cttcgcgcgg cggggtgaag ccgacgatgg tgcggatcag cgtggtcttg
     6841 cccatcccgt tgcggcccag gatggccagc acctcgcccg cctccacccg cagcgagatc
     6901 ccctgcagca cgtggctctc gccgtagtag gtgtggacgc cctccacctc aagcgccaag
     6961 gtagatctcc ttcacctgcg ggtcggcctt cacctgctcg cgcgggccgt cggcgatgac
     7021 gcggccgtag tgcagcaccg tgaccatctc ggacagctcg agcgcgatat ccatgtcgtg
     7081 ctcgatcatc agcacggtga tctcgggatc gagccggcgc agcaggccgg ccatcagccg
     7141 cgactccgcg ggcgacaggc cggcggtggg ctcgtccagc agcagcacgc gcggctgccc
     7201 ggcgagggcc agcgcgatct cgagctgtcg ctgctcgccg tgggagaggt tgcgcaccac
     7261 cgcgccgccg agcttctcca gctcgaggcg ctggagcgcg acccgcgccc gctcgagcag
     7321 ggccggatag cgccccaccg ggcggagcat cgagaagcgc accggcgagt gcgcctgcac
     7381 cgcgagcagg cagttctcca gcacgctcag atcggggaag aggttggtga tctggaacgt
     7441 gcgcgccagg ccgagcgcgg cgcgctggtg cgcgggggcg tgcgtgacgt cgcgcccgaa
     7501 cagggtgatg cggcccgagc tgagcggcat cgcgcccgcg atgaggttga agagcgtggt
     7561 cttccccgcc ccgttagggc cgatgagcgc acggcgctcg cccggacgga cggccagcgt
     7621 cacgccgtcc acggcctgga ggcccccgaa ggcgcggcag acggcgtcga ggtgaagcgc
     7681 gtgatcggtc atcggcgccg tccgcgcagg gcaccgacga tgccggccgg ggcgaagagg
     7741 atcacgccga tgtagacgcc gcccaggatg agcagccagc gcttggtgta gacgctgacg
     7801 aagttcttga ggaacacgat gatcccggcg cccagcgcgg ggccgatcag cgtgcccgag
     7861 ccgccgagcg ccaccatcag cagcgcctcg accgaggtca cgagctgcac gtcgttgggg
     7921 ctgacgaagc cgttgtagta ggcccagagc gtgcccgaga agccgccgaa gacgcccgag
     7981 agcacgaacg cgatgtactt gtgcagccac acgttgtagc cgagggcctg catgcgcccc
     8041 tcgctgccgc ggatgccccg cagcccgagg ccgaacggcg agatcacgag caggcccatc
     8101 agcacccagg ccatcacggc gcaggccagg cagaagtagt agaacggcac cgccgcgatc
     8161 agctcgatgc cgagcagggt cggtcgcggg atgcccgcga tgccgttatc gcccttggtg
     8221 agcgacaccc agcgataggc gagcccccag atcaccatgc tgagggccag cgtgatcatc
     8281 aggaagtagg tgccggtggc gcggatggca agcagcccga agaccgccgc ggtgagggtg
     8341 gccagcgcca cgccggcggc gaggcaggcg agcaggccca tctggtgctc ggtggtcatg
     8401 atggcgaccg cgtaggcgcc caccccgaag tacgcggcgt ggccgagcga ggagaggccc
     8461 gtgtagccga gcagcacgtc gaggctcatg gccaggatgg cgacgatgag cgcctgggtg
     8521 aggacggtga gcgggtaggc cggcagccag gggcccgcca cggcgagcac cgcgatgagg
     8581 accgcggcga gcagcgcgcg gcgcccgatc atccgcggcc gaagaggccc gtcggccgga
     8641 tggccaggat gatcgccatc ggagcaaaga gggtgaagta ggacagctcg gggaagagcg
     8701 ccttgccgaa gttgtcgagc aggcccacca gcaggctccc cgccaccgcg ccgggcaggc
     8761 tgccgagccc gcccacgatc accaccacga aggcgtaggg cagcacctcg aagtccgcgc
     8821 ccgggtagac gccgaggaac ccgccgccga tgaccccgcc cagcgcggcc aggccggcgc
     8881 ccagcgcgaa caccgccagc gacacgcgcg gcacgttgat gcccacgccc tgggccatct
     8941 cggcgtcatc caccgcggcg cggatcatgg cgccgacccg ggtgcggtcc atcgcgagcc
     9001 acagcgccag ccccacgacc accgcgacgg ccacgatgaa gatgcggaag atcgggaaca
     9061 ccgcgccgcc cacccgcacg gtcccggtca gcatcttcgg gatcggcacc gtgtacgggt
     9121 ccccgcccca gatcagcagg gccaggtcct ggaagatcag cgcgaaaccg atcgtcatca
     9181 gcacctggcc gagcacctcg ccgcgcagcc ggcgcaggaa gaagcgctcc atgcccatcc
     9241 cgacgagcgc gatggccacc gcccccgcga ggcacgcgag cgcgaagctc cgcgtgtaca
     9301 acgccaccga gagccccacg tagccgccca gcatgaagta ggagccgtgc gccaggttca
     9361 ctattctcat gacgccaaag attagcgaga ggccgctggc cagcaggaag aggagcgcgc
     9421 cgtaggagag cccgttgaac gactgcgcga tccagaactc ggcgctcacg ccccgagccc
     9481 gatcctagcg gacgcgcagc aggcgggcgg cgttctcgcg cacgaccttg gcctggtcgg
     9541 cccgcgagag cttgaccgct ttgtcgcgga tgatgtcgag ggggcgctcg tagcccatgt
     9601 cgaagcagaa atcggtgccg agcatcaccc ggtccgcgcc caccgtgtcg atgagatacc
     9661 gcagcgcgcc cgcatcgtgg ctgatggtgt cgtaggtgaa ccggcgcagg taggcccgca
     9721 cgggcttctt cgccgccttg ttcgcttccg gccgcacctt ctgcccgcgc tgcagccgcc
     9781 cccacaccag cgggaacgtg ccgcccgcgt gcggcaggat cacctgcagc ttcggcagcc
     9841 gatccagcac cccgccgaag atgaggtggg ccgccgcgat cccggtgtcg tacgggttgc
     9901 cgagcaggtt ggtcaggtag aagggcttga ggcgttccgc cccattcacg ttgaagggat
     9961 ggagccccac cggcagcccc agcgcctggc agcgctcgaa caccgggaag aactcggggt
    10021 cggagaggtt gcggccgttg acgttggtgc ccatgtacac cgcgcggacg ccgggcagcc
    10081 tcgcggcccg gtccagctcg gccagggcca gcgtcgtgtc ctgcatcggc agcgtggcgc
    10141 agacgacgaa gcggtcggga taggcggtgt gcgcggcgct ggcgccgtcg ttccattcgc
    10201 gcgccagccg ccgaccgagc tcgccctggg cccagtagac catcggcgcg gtcagcgaga
    10261 gggcctgcac ggccacgccc tgccggttca tggaggccac gcgcttcggc aggtcgtaat
    10321 agcgcgcctc gagcggcgcc gtgcgtgcgc ccttcacgtc gacgacgggc ccaaccgggc
    10381 tcgagcggtc gatgcggccg ccggatgggg cgccgccgtc ctcgatcgcg cggaggaacc
    10441 gctcggggaa gaagtgcgcg tggacgtcga tgcccagggg cgctgccacg gcggggattc
    10501 tactatagaa gcggacggga agcctggtcg tctagtccgt cgtcctactg gctcggcagg
    10561 accaggcgga acttgttcgg gtccgccttc accaccgcgt cggcgtagtc gcgatcgaag
    10621 ttgccgcgcg ccccggacgg gctgtgctcg agcacctcga cgccgcgaag atagtagctg
    10681 aagcccttgt tggcgcggtc gtaggcctcg ctctgcccgc gcgcggccga gcgcaggatc
    10741 tcgccggtct ggcgcatcgt gttggcggca tcctggaggc ggtcgccatc ctaggacggt
    10801 gctccgggcg agtcaacacc tactcctcgg cgaccgtgac ggggatgacc tcgacctccg
    10861 aatactccgt gtcgcagatc ggctgaccga ctggttcgcc gccctcttca tggacgacct
    10921 gccggatatg ccgggcaaac atgtcgcgca cgaggcgtcc caccacctgt cccacccgat
    10981 tgacatcctc cgcgacgaat ccggctcggg ggtcgataca cccagttgat gtcccgccac
    11041 atagttccgt ttcttggccg ttgtcgcccc ggatgaacgt gacgaggttg accccgtaag
    11101 tgacgaacac gaagtccgcc agcccgtcgc ccaacgaaac gctctgctgg aagaacccgc
    11161 gcggctgatg cgcaaagagc ggcacctccg tgcatggctc gatgcgttcc ggccacgacg
    11221 ccaggatcag cggaaccgga atggtcacct gtcgttgcgc atcaaggtcc gtcgtctgga
    11281 cgatcctgcc accctcgatg ctgctcgaga acgtgctctc ccccgcccag tacatcggga
    11341 agagattgac agccgggatg aacaagaccg tcttccccgg cctccaggcc agctccgccc
    11401 gcccgtcctg gtcggtgaga cctgagccga ggaccgctcc gctctggacg tcgatagcgt
    11461 ggacggcggc cccccgatgg ggtgttccgt cactgcgctt gatcagtcgg acgaccatca
    11521 ttggctcgat gacgacctgg cgccccacta ccgcgtcgcg ctcactcccc agcgagccgc
    11581 gaaacaccaa catgtacctc ccgggctcct tcggcatcgg gctgctcggc gccgacacct
    11641 tcaggtcttc cacgagcccg gatggcgcaa gcgctctcgt ccactgggcg cccggcacgg
    11701 ggcgtcgaac gtcgctgacg tcgtcgtagt acagggtgaa cgtcccgtcc atggcctcgt
    11761 cagtgccgtt ggcaaacccg aacgacagat cgttgccgaa tgcgatcacg agccctcgaa
    11821 agaagtagtc cagcagcgcc gtcgagtacg cgatcgctcg cggtacgagc cgctccgcat
    11881 agtcgcgata gacggcctca tcgagggctg ggccctgccg gaaccgcgtc cagtccaggt
    11941 tgaaacgctg ctgataatcg cggagcagcc cgacggtggc caggcgatag ccggtggcgc
    12001 cgtccgcggt cttcacgaag tagcggcgct tggcggtcga gccgcctacg ctcacgtcga
    12061 agtcctgctc catcacgctc gatcggttcg ggtacggaaa ccgcttcaat ggatcgacgt
    12121 cgttctccgt gaagatccga tcttcgctga agaagttcgc gttcgtatat tcggcaaggc
    12181 cggtgagggc cgccatggtc aaccctggat tgccgcccgc ataccggtcg gtatcggtca
    12241 gccgggcgat cgcgaccggt gctcggggat cgctgtcgag cgcactccag cggaggtccg
    12301 ggacatcggg tgtgtcgggt
//
LOCUS       DASUFA010000156        11798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1622807, whole genome shotgun sequence.
ACCESSION   DASUFA010000156 DASUFA010000000
VERSION     DASUFA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622807"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1214
                     /locus_tag="VFX87_05585"
     CDS             153..1214
                     /locus_tag="VFX87_05585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4994417.1"
                     /translation="MRVETLLPLGKLDPGLRASDTPLELGRVIEDARLVESLGYDGLA
                     VEETKVDPYVVMALAAAGTTRLRLTTAVAMAFPRSPTVTAMSAWNIQKLSRGRFVLGL
                     GSQVRGHITRRFGLPYRPPGPWMRDYVRAVRAVWTAWQRRTPLAFESPHYNLSLMVPL
                     FDPGPIEHPDIPIHVAAVNPTMCRFVGEVADGLRPHPVCTPRYIRDVMLPAMRAGAGA
                     AGRSSALSVAMKPLVCAAPDEATLAARVRDVRARVAFYASTPGYRAAFEVHGLGELAA
                     ELAVLSRAQRWEEMPSRIDDEVLHTYAVVGTYAEIGRKLVERYRGLVTDVEFSIPAAT
                     PGEQGILRELIQEIRRETA"
     gene            complement(1276..1893)
                     /locus_tag="VFX87_05590"
     CDS             complement(1276..1893)
                     /locus_tag="VFX87_05590"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX4994418.1"
                     /translation="MELDPQTMKSESTYKLLIGCVVPRPIAWVSTVGADGVNNLAPFS
                     FFMGVCQDPPTIAFSSGPRAKNRKDTVRNAEHTGDFVVNVVDDDRAEQMNLSSGEYPP
                     EVDEFALTGLTAAPGVRVRSPRVVQAPISLECRVAQIIPVGRGPHSLVLGEIVYFHVR
                     DDVYDAATGRIDMARLKPVGRLAGHQYTYVHEIFEMKRPNENYRG"
     gene            complement(1924..5058)
                     /locus_tag="VFX87_05595"
     CDS             complement(1924..5058)
                     /locus_tag="VFX87_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX4994419.1"
                     /translation="MNLSGPFIRRPVGTTLLTIALVLAGAIGFQLLPVAPLPQVDFPT
                     INVSTGLPGASPLTMASAVATPLERQFTRIAAVSEMTSTSFLGSTSVALQFDLSRDIN
                     GAARDVQSAINAASGQLPSNLPSNPTYRKVNPADAPIMILALTSATATPGKLYDMAST
                     ILQQKLAQIEGVGQVFVGGSSLPAVRVELNPHALARYGIGLEDVRAVLASANVNRPKG
                     QIHGPERAWEIRTSDQIRYAAEYQPLLVAWRNGAAVRLEDVADVRDSVEDLRSIGLAN
                     GQRAALLIIFRQPGVNVIDTVDRIRQAQPELHALLPADVTLTPVLDQTVTIRASVSDV
                     EHALMISVALVVLVVFLFLRDWRATAIPGVVVPVSLIGTFAFMYLIGYSIDNLSLMAL
                     TVATGFVVDDAIVVVENVMRHLEAGMSPLDAAVQGAREIGFTVLSISISLVAVFIPIL
                     LMGGLIGRLFREFAVVLSVAILVSLVVSLTVTPMMCARLLRSRESTPRGRLYRAGERV
                     FGWVTAGYEKSLGWALYYPRTVLLIAGLTCAVNVYLFVAIPKGFFPQQDNGRLSGSIV
                     AAQDTSFQAMRDKVIRYAEIVRGDPAVATVTAYTGGGARGTTVNTARMFIALKPRRER
                     DATADGVITRLRPKLARVAGATCYLQAVQDIRVGGRMGNAQYQYTLQADRLEDLSEWA
                     PRMLETLRKVPELRDVSSDQQDAGLQVPLTIDRATAARLGVSTRLIDETLYSAFGQRQ
                     VSTIYTQLNQYRVVMEVAPEFWQSPDALRNIHIQSARGESVPLTALTRFEPSAAPLQV
                     NHQGLFPSVTLSFNLAPGVALGEAVTAVEGAARRIALPASIRGKFAGAAQAFQASLST
                     QPLLILAALVAVYIVLGTLYESFVHPLTILSTLPSAGVGALLALLACGMELSIIALIG
                     IILLIGIVKKNAIMVIDVALELERREGRAPREAIHEACLRRLRPILMTTMAALLGALP
                     LALGTGTGSELRRPLGVSIVGGLLLSQLLTLYTTPVVYLFLERWRLRTVSGWRKLGER
                     LGGRRPVEQISGGK"
     gene            complement(5055..8171)
                     /locus_tag="VFX87_05600"
     CDS             complement(5055..8171)
                     /locus_tag="VFX87_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX4994420.1"
                     /translation="MNPSRVFILRPVATSLLTVAIVLAGIVGYRLLAVSALPQVDYPT
                     MQVQTFYPGASPDVMASAVTAPLERQFGQMPGLKQMTSTSSSTASVITLQFDLNLALD
                     VAEQQVQAAINAATSFLPGDLPNPPIYSKVNPADAPILTLGLTSASLPLTTVQDLADT
                     RLVPKISQLPGVGLVGLSGGQKPAIRVQVNPARLAANGLTLEDVRTAVASANVNQAKG
                     SLDGSRQAFTIGANDQLTSSDQYRNLVVAYRNGAPVHLSDVADTVDDAENLRQAAWMG
                     VEPAVIVNIQRQPGANLIQVVDRIKQLLPQLQTTLPGSVKVAILTDRTTTIRASVHDV
                     QIELMLAVILVVLVIFLFLGSPAATFIPSIAVPVSIIGTFAVMYGLGFSLNNLSLMAL
                     TIATGFVVDDAVVMIENIARYIEAGDAPLEAALKGSAQIGFTILSLTVSLIAVLIPLL
                     FMGDIVGRLFREFAITLSAAILVSAAVSLTLTPMLCAKLLRPHEERRPGRVARASQAV
                     FNRVLGLYARTLRVVLDHQPATLLVAVATLGLTLLLYVAVPKGLFPIQDTGVILGISE
                     APQSVAFPAMAQRQRALAQAILRDPAVDTLSSFIGIDGTNVTLNSGRLLITLKPLEER
                     RASVREIIDRLRPRLAEVSGITLYLQPVQDLTVEDRVSRTQYQYSLENPSVDELNTWA
                     PRLLERLRALPELQDVASDQQDQGLETSIRIDRGTASRLGITPQMIDDALYNAFGQRQ
                     ISTIFTQLNQYRVVLEVKPEYRQDPDALKQIYLRSAGGGQVPLSTFTVMEERATPLLV
                     SHQGQFPAVTVSFNLAPRVSLGSAVDVIEQARRDLKMPAGIETRFQGAARAFQASLAN
                     EPLLILAAVCTVYIVLGVLYESWIHPVTILSTLPSAGVGALLALMAMRLDLSVIALIG
                     IILLIGIVMKNAIMMIDFALDAQRTEGKPAPEAIYQACLLRFRPIVMTTMAALLGGVP
                     LALGQGVGSELRQPLGIAIVGGLIMSQFLTLYTTPVIYLAFDRLAARATRRRAASAGS
                     LAAAEPRP"
     gene            complement(8168..9331)
                     /locus_tag="VFX87_05605"
     CDS             complement(8168..9331)
                     /locus_tag="VFX87_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEX4994421.1"
                     /translation="MAGRARRFWVICAILSVLIAAGAYYAVARPKRLQPAAPAAGPRP
                     VPVVTVTARSGDLGVYLNGLGSVTPLSIVTVRSRVDGQLMRTLFQEGQIVRAGDLLAE
                     IDPRPFQTQLTQAEAQLAKDAAALSNARMDLERYRTLVKGGFIPQQQLDTQASAVAQL
                     AASIQADQGQIDSIRLQLTYTRIHAPITGRAGLRLVDPGNMIHANDPGGLVVLAQVEP
                     IAVVFTIPQDNLPPVLARQRAGGPIPVDAYDREGRTRLATGILVAVDNQIDPTTGTVK
                     LKASFTNSDGSLFPNQFVNARILVDSLRGAVLVPNEAIQRGAQGPFVYLVKPDKTVEL
                     RKVTPGPSEAGVTAVREGVAAGDALIVDGADRVQERSRVEATSRERPAARAAP"
     gene            complement(9392..10300)
                     /locus_tag="VFX87_05610"
     CDS             complement(9392..10300)
                     /locus_tag="VFX87_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4994422.1"
                     /translation="MLDSRSDLWAIVLAGGEGVRLRSLARELYGEARPKQYCALAGSK
                     SLLRTTLDRVARLIPPSQTVVVTQASHAGYVADELGDHPAVTVLAQPCDRGTAAAVLL
                     AAHWVRTRDPGGVVAVFPTDHFIAEESLFMSHVAAAGGYVRNHPAWLLLLGVRPIEPE
                     PDYGWIEPGEWVGRTAAGAVRRVRAFHEKPSADVARRLQGAGGLWNTFVFAATAAALV
                     EAGLACLPLLHDRLTRLDLFTGTRYETLALRQAYLFAPTADFSRAVLASSEIPLAVAE
                     IPAVTWRDLGTPERVARSLGARGRRE"
     gene            complement(10368..10817)
                     /locus_tag="VFX87_05615"
     CDS             complement(10368..10817)
                     /locus_tag="VFX87_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994423.1"
                     /translation="MFPIFKVMLASFAVAALGVAGLDAHVARADWRDPGLDWDESAVV
                     VKAFDPDDASALADHFFHVEWAVGPGAPGRARITGYVYNDGGDSAVDVALRVHEIDAS
                     GQDLASVVAPVGDAVPAHGRAYFDVGVPDSRFYRVDVTSFDFVELSN"
     gene            complement(10877..11161)
                     /locus_tag="VFX87_05620"
     CDS             complement(10877..11161)
                     /locus_tag="VFX87_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994424.1"
                     /translation="MKRLLVALLLLAPVALPGLAFASPPDPTWIQGIYDDADGDDVVT
                     LIASGTGHTPPAPPVLRFVAHLIVRLAPTPERQPLALWASAALPRAPPAP"
     gene            complement(11163..11669)
                     /locus_tag="VFX87_05625"
     CDS             complement(11163..11669)
                     /locus_tag="VFX87_05625"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX4994425.1"
                     /translation="MTNTLTIRTILAATLLVVSTATAAFAQSPPPGFGAPLGGMRGSM
                     GSTDGELWLMVRAASLTPEQQAKVRGILSTHRATTRPLIEQLRQAQQELGAKLLAPGP
                     LQPADLQPLLGRIGQLRDQLAQDGAQAALEVRAVLTPEQLARAAQTKERLTQLREEMR
                     QLMQPPRP"
BASE COUNT         1718 a         3921 c         4527 g         1632 t
ORIGIN      
        1 cacggctggc cggcgggaag atcgtccagc atgctggcgc ggtccagctc gaggtagccg
       61 ctgcaaaggg cgaagaccga gatcacggcg gctacgataa accctcgcgc gcccgttgtc
      121 aggtggaaat gcatccgcta gcatgacggc ctgtgcgcgt cgagacgctc ctcccgctcg
      181 gcaagctcga ccccggactc cgggcctccg acactccgct cgagctcggc cgcgtgatcg
      241 aggacgcccg gctcgtcgag tcgctcggct acgacggcct cgcggtggag gagaccaagg
      301 tcgatcccta cgtggtcatg gcgctggccg cggcgggcac cacgcgcctg cgcctcacca
      361 ccgcggtcgc gatggcgttc ccgcgcagcc ccaccgtcac cgcgatgtcg gcgtggaaca
      421 tccagaagct ctcgcgcggg cgcttcgtgc tggggctcgg cagccaggtg cggggacaca
      481 tcacgcggcg cttcgggctg ccgtaccgtc cgcccgggcc gtggatgcgc gactacgtcc
      541 gggcggtgcg cgcggtgtgg acggcgtggc agcggcggac gccgctcgcc ttcgagagcc
      601 cgcactacaa cctgagcctg atggtcccgc tgttcgaccc ggggccgatc gagcatccgg
      661 acatcccgat ccacgtggcc gcggtgaacc ccaccatgtg ccgcttcgtg ggggaggtgg
      721 ccgacggcct gcgtccgcat ccggtgtgca cgccgcgcta catccgcgac gtcatgctgc
      781 ccgcgatgcg cgcgggcgcg ggggcggcgg gacgcagctc cgcgctctcc gtcgcgatga
      841 agccgctcgt ctgcgcggcg cccgatgagg cgacgctggc cgcgcgggtg cgggacgtgc
      901 gggcgcgggt ggccttctac gcctccaccc cgggctaccg cgccgccttc gaggtgcacg
      961 gcctgggcga gctggccgcc gagctggccg tgctgtcgcg ggcccagcgc tgggaggaga
     1021 tgccgtcgcg catcgacgac gaggtgctcc acacgtacgc ggtggtgggg acctacgcgg
     1081 agatcgggcg gaagctggtg gagcgctacc gaggcctcgt caccgacgtg gagttcagca
     1141 tccccgccgc gacccccggc gagcagggca tcctgcgcga gctgatccag gagatccggc
     1201 gcgagacggc gtgatccgcg ccgggtcctg cacgccccgg aggaccggcg cgatctcgtc
     1261 tgatcggccg gctcgctacc cccggtagtt ctcgttgggc cgcttcatct cgaagatctc
     1321 gtggacatag gtgtactggt ggccggcgag gcgcccgacc ggcttgaggc gcgccatgtc
     1381 gatgcgcccg gtggccgcgt cgtagacgtc gtcgcgcacg tggaagtaca cgatctcgcc
     1441 cagcaccagg ctgtggggcc cgcgcccgac cgggatgatc tgggccacgc ggcactcgag
     1501 gctgatcggg gcctggacca cgcggggcga gcgcacccgc acgccggggg cggcggtgag
     1561 gccggtgagc gcgaactcgt ccacctcggg cgggtactcc cccgagctca ggttcatctg
     1621 ctcggcgcgg tcgtcgtcca cgacgttgac cacgaagtcg ccggtgtgct cggcgttccg
     1681 cacggtgtcc ttgcgattct tcgcgcgcgg ccccgaggag aacgcgatgg tcggcgggtc
     1741 ctggcagacc cccatgaaga agctgaaggg ggcgaggttg ttcacgccgt ccgcgcccac
     1801 cgtggagacc cacgcgatcg gccgcggcac cacgcagccg atcagcagct tgtaggtgga
     1861 ctcggacttc atggtctgcg gatcgagctc catggcgatg gtcttctccc gggatgaagg
     1921 tggttacttg ccgccgctga tctgctcgac gggccgccgc ccgccgagcc gctcgccgag
     1981 cttgcgccag ccgctgaccg tgcgcaagcg ccagcgctcg aggaacagat acacgacggg
     2041 cgtcgtgtag agcgtgagga gctggctcag gagcaggccg cccacgatgc tcacgcccag
     2101 cgggcggcgc agctcggagc cggtgccggt gccgagcgcc aggggcaggg cgcccagcag
     2161 cgcggccatg gtggtcatca ggatcggccg cagccggcgc aggcaggcct cgtggatggc
     2221 ctcgcggggg gcgcggccct cgcgccgctc gagctcgagc gcgacgtcga tgaccatgat
     2281 cgcgttcttc ttcacgatgc cgatcagcag gatgatgccg atcagggcga tgatcgacag
     2341 ctccatcccg cacgcgagca gcgcgagcag cgcgcccacc cccgccgagg gcagggtgga
     2401 caggatggtg agcgggtgga cgaagctctc gtagagcgtg ccgagcacga tgtagaccgc
     2461 gacgagggcg gccaggatca ggagcggctg cgtggacagc gaggcctgga aggcctgcgc
     2521 cgcgcccgcg aacttgccgc ggatgctcgc gggcagcgcg atgcggcgcg ccgcgccctc
     2581 caccgcggtg accgcctcgc cgagggccac cccgggggcc aggttgaacg acagcgtcac
     2641 ggacgggaac aggccctggt ggttcacctg cagcggagcc gcgctcggct cgaagcgggt
     2701 gagggcggtg agggggacgg actccccgcg cgccgactgg atatggatgt tgcggagggc
     2761 gtcggggctc tgccagaact cgggcgccac ctccatcacc acccggtact ggttgagctg
     2821 ggtgtagatg gtggacacct ggcgctggcc gaaggcgctg tagagcgtct cgtcgatgag
     2881 gcgcgtgctc accccgagcc gggccgcggt ggcccgatcg atggtgagcg gcacctgcag
     2941 ccctgcgtcc tgctggtcgc tcgagacgtc gcgcagctcg gggaccttgc gcagggtctc
     3001 gagcatccgc ggggcccatt cgctcagatc ctccagccgg tcggcctgca gcgtgtactg
     3061 gtactgcgcg ttgcccatgc gcccgcccac ccggatgtcc tgcaccgcct ggagatagca
     3121 ggtggccccg gccacccggg cgagcttcgg gcgcaggcgg gtgatcaccc cgtcggcggt
     3181 ggcgtcgcgc tcgcggcgcg gcttcagcgc gatgaacatg cgggcggtgt tgacggtggt
     3241 gccgcgggcc ccgccgccgg tgtaggcggt gacggtggcc accgcgggat cgccgcgcac
     3301 gatctccgcg tagcggatca ccttgtcgcg catggcctgg aacgacgtgt cctgggcggc
     3361 cacgatcgag ccgctgaggc ggccgttgtc ctgctggggg aagaagccct tggggatcgc
     3421 cacgaagagg tacacgttga ccgcgcaggt caggccggcg atcagcagca cggtacgcgg
     3481 gtagtagagc gcccagccga gcgacttctc gtacccggcc gtgacccagc cgaacacccg
     3541 ctcgccggcg cggtagaggc ggccgcgggg cgtgctctcg cgcgagcgga gcagccgtgc
     3601 gcacatcatc ggggtgacgg tgagcgagac gaccagcgag accaggatgg cgacggagag
     3661 caccaccgcg aactcgcgga agaggcgccc gatcagcccg cccatcagga ggatcgggat
     3721 gaagacggcg accagcgaga tgctgatcga gagcacggtg aagccgatct cgcgcgcgcc
     3781 ctggaccgcc gcatcgaggg gcgacatgcc cgcctcgaga tgccgcatga cgttctcgac
     3841 caccacgatg gcgtcgtcca ccacgaaccc ggtggccacc gtgagggcca tcagcgacag
     3901 gttgtcgatg ctgtagccga tcaggtacat gaaggcgaag gtgccgatga gggacaccgg
     3961 caccaccacg ccggggatcg cggtggcgcg ccaatcgcgc aggaacagga acaccaccag
     4021 caccaccagg gccaccgaga tcatcagcgc gtgctcgacg tcggagaccg aggcgcggat
     4081 ggtcacggtc tggtcgagca ccggagtcag ggtcacgtcg gcgggcagga gcgcgtgcag
     4141 ctcgggctgc gcctgccgga tgcggtccac cgtgtcgatc acgttgacgc cgggctggcg
     4201 gaagatgatg agcagcgcgg cccgctggcc gttggcgagg ccgatggagc gcaggtcctc
     4261 gacggagtcg cgcacgtcgg ccacgtcttc caggcggacc gcggccccgt tgcgccaggc
     4321 cacgagcagg ggctggtatt ccgccgcgta gcggatctgg tcgctggtgc ggatctccca
     4381 ggcccgctcc ggcccgtgga tctggccctt cggccggttg acgttggcgc tcgccagcac
     4441 cgcgcgcacg tcctcgagcc cgatgccgta gcgcgcgagc gcgtgggggt tcagctccac
     4501 ccggaccgcg ggcagcgagc tgccgcccac gaagacctgc cccacgccct cgatctgggc
     4561 cagcttctgc tggaggatcg tggaggccat gtcgtagagc ttgcccggcg tcgcggtggc
     4621 ggaggtcagc gccaggatca tgatgggcgc gtcggccgga ttgaccttgc ggtaggtggg
     4681 gttgctcggg agattcgagg gaagctggcc gctcgccgcg ttgatggccg actgcacgtc
     4741 gcgggcggcg ccgttgatgt cgcgggagag gtcgaactgg agcgccacgc tcgtcgagcc
     4801 caggaagctc gtggaggtca tctcgctgac cgccgcgatg cgcgtgaact ggcgctcgag
     4861 cggggtggcc accgcggagg ccatggtgag cgggctcgcc ccgggcaggc cggtggagac
     4921 gttgatggtg gggaagtcga cctgcggcag cggcgccacc ggcaggagct ggaagccgat
     4981 ggcgcccgcc agcacgagcg cgatcgtgag gagcgtggtg ccgaccggcc gccggatgaa
     5041 ggggccggag aggttcacgg gcggggctcc gcggcggcga gggagcccgc ggaggcggcg
     5101 cggcgccggg tggcgcgcgc ggcgagccgg tcgaaggcga ggtagatcac cggcgtggtg
     5161 tagagcgtga ggaactggct catgatgagc ccgcccacga tcgcgatgcc gagcggctgg
     5221 cgcagctcgg agccgacgcc ctggcccaac gccagcggga cgccgcccag cagcgcggcc
     5281 atcgtggtca tcacgatcgg gcggaagcgc agcaggcagg cctggtagat cgcctcgggc
     5341 gcgggcttgc cctcggtgcg ctgggcgtcg agcgcgaagt cgatcatcat gatcgcgttc
     5401 ttcatcacga tgccgatcag caggatgatg ccgatcagcg cgatcacgct caggtcgagg
     5461 cgcatggcca tcagggcgag cagggcgccc acccccgcgg agggcagggt ggacaggatg
     5521 gtcaccgggt ggatccagct ctcgtacagc acgccgagca cgatgtagac ggtgcacacc
     5581 gcggccagga tcagcagagg ctcgttggcg agcgaggcct ggaaggcgcg cgcggcgccc
     5641 tggaagcgcg tctcgatgcc cgccggcatc ttgagatcgc gccgggcctg ttcgatgacg
     5701 tccaccgccg agccgagcga cacgcggggc gccaggttga aggagacggt gaccgccggg
     5761 aactgcccct gatggctcac cagcagcggg gtggcccgct cctccatgac ggtgaaggtc
     5821 gagagcggga cctggccgcc gcccgccgag cgcaggtaga tctgcttgag cgcgtccgga
     5881 tcctggcggt actccggctt gacctcgagc acgacgcggt actggttgag ctgggtgaag
     5941 atcgtcgaga tctggcgctg gccgaaggcg ttgtagagcg cgtcgtcgat catctggggc
     6001 gtgatgccga ggcgcgacgc ggtgccgcgg tcgatgcgga tggacgtctc gagcccctgg
     6061 tcctgctggt cgctcgccac gtcctgcagc tcgggcaggg cccggaggcg ctccagcagg
     6121 cgcggggccc acgtgttgag ctcgtccacg ctcgggttct ccaggctgta ctggtactgg
     6181 gtgcggctca cccggtcctc caccgtgagg tcctgcaccg gctggaggta gagcgtgatg
     6241 ccgctcacct cggcgaggcg cgggcgcagg cggtcgatga tctcgcgcac cgacgcccgc
     6301 cgctcctcga gcggcttgag cgtgatcagc aggcgcccgc tgttgagcgt cacgttggtg
     6361 ccgtcgatgc cgatgaacga cgacagcgtg tccaccgcgg gatcgcgcag gatggcctgg
     6421 gcgagggcgc gctggcgctg ggccatggcc gggaaggcga cgctctgggg cgcctccgag
     6481 atgcccagga tcaccccggt gtcctggatg gggaagaggc ccttgggcac cgccacgtac
     6541 aggaggaggg tgagcccgag cgtcgccacc gcgaccagca gggtcgcggg ctgatgatcg
     6601 agcaccacgc ggagcgtgcg cgcgtacagg ccgaggaccc ggttgaacac ggcctgggag
     6661 gcgcgggcca cgcggccggg cctgcgctcc tcgtgggggc gcaggagctt cgcgcagagc
     6721 atgggcgtca gcgtcaggga caccgcggcc gagaccagga tcgccgcgct gagcgtgatc
     6781 gcgaactcgc ggaagaggcg ccccacgatg tcgcccatga agaggagcgg gatcagcact
     6841 gcgatgaggg agacggtcag cgagaggatc gtgaagccga tctgggccga gcccttcagc
     6901 gcggcctcca gcggcgcgtc gcccgcctcg atgtagcgcg cgatgttctc gatcatgacc
     6961 accgcgtcgt ccaccacgaa gccggtggcg atggtgagcg ccatcagcga gaggttgttc
     7021 aggctgaagc cgagcccgta catcaccgcg aaggtgccga tgatggagac cggcaccgcg
     7081 atggacggga tgaaggtggc ggcggggctg cccaggaaga ggaagatcac gaggaccacc
     7141 aggatcaccg ccagcatcag ctcgatctgc acgtcgtgca ccgaggcccg gatggtggtg
     7201 gtgcggtcgg tgaggatcgc gaccttcacc gagccgggca gggtggtctg gagctgcggc
     7261 aggagctgct tgatacggtc caccacctgg atgaggttgg cgcccggctg gcgctggatg
     7321 ttgacgatca ccgcgggctc cacgcccatc cacgcggcct gccgcaggtt ctccgcgtcg
     7381 tccaccgtgt cggccacgtc ggagaggtgc accggcgcgc cgttgcggta ggcgaccacg
     7441 aggttccggt actggtcgct cgacgtgagc tggtcgttgg ccccgatggt gaacgcctgg
     7501 cggctgccgt cgaggctgcc cttggcctgg ttcacgttgg ccgaggccac cgcggtccgc
     7561 acgtcctcga gcgtgagccc gttggcggcc aggcgggcgg gattgacctg gacgcggatc
     7621 gccggcttct ggccgccgct cagaccgacc aggcccacgc cgggaagctg ggagatcttc
     7681 ggcaccaggc gcgtgtcggc cagatcctgc acggtggtga gcggcagcga ggcggaggtg
     7741 aggcccagcg tgaggatcgg cgcgtcggcc gggttgacct tgctgtagat cggtgggttg
     7801 ggcaggtcgc cggggaggaa cgacgtggcc gcgttgatgg cggcctgcac ctgctgctcc
     7861 gcgacgtcga gcgccaggtt gagatcgaac tggagcgtga tgaccgaggc ggtgctggag
     7921 ctggtggacg tcatctgctt gagcccgggc atctggccga actggcgctc gagcggcgcg
     7981 gtgaccgcgg aggccatcac gtccgggctg gcccccgggt agaatgtctg tacctgcatg
     8041 gtcgggtagt ccacctgggg cagggccgag accgcgagca gccggtagcc gacgatcccg
     8101 gcgaggacga tggcgacggt gaggagcgag gtggcgaccg gccggaggat gaagacgcgc
     8161 gacgggttca tggagccgcg cgcgcggcgg gccgctcgcg gctcgtggcc tccacgcgcg
     8221 agcgctcctg cacgcggtcg gccccgtcca cgatgagggc gtcgcccgcg gccacgcctt
     8281 cgcgtaccgc ggtgacgccc gcctcgctgg gcccgggcgt gacctttcgc agctcgaccg
     8341 tcttgtccgg cttcaccagg tagacgaagg gcccctgggc cccgcgctgg atcgcctcgt
     8401 tcggcaccag caccgcgccg cggagcgagt ccaccaggat gcgcgcgttg acgaactggt
     8461 tcggaaacag cgagccgtcg ctattggtga aggaggcctt gagcttgacg gtgccggtcg
     8521 tcgggtcgat ctggttgtcc accgcgacca ggatgccggt cgccagcctg gtgcggccct
     8581 cgcgatcgta ggcgtccacc gggatgggcc cgcccgcgcg ctggcgggcg agcacgggcg
     8641 gcaggttgtc ctgggggatc gtgaacacca ccgcgatcgg ctcgacctgg gcgagcacga
     8701 cgagcccgcc cggatcgttg gcgtggatca tgttgccggg atccacgagg cggagcccgg
     8761 cgcggccggt gatcggggcg tggatgcgcg tgtaggtgag ctggaggcga atgctgtcga
     8821 tctgcccctg gtcggcctgg atggacgccg cgagctgggc gaccgcggag gcctgggtgt
     8881 ccagctgctg ctgcgggatg aagccgccct tgaccagggt gcggtagcgc tcgagatcca
     8941 tgcgcgcgtt ggacagcgcg gcggcgtcct tggcgagctg cgcttccgcc tgggtgagct
     9001 gcgtctggaa ggggcgggga tcgatctcgg ccagcagatc gcccgcccgc acgatctggc
     9061 cctcctggaa cagcgtccgc atgagctggc cgtccacccg gctgcggacc gtgacgatgc
     9121 tgaggggggt gaccgagccg agcccgttga ggtagacgcc gagatcgccg ctgcgggcgg
     9181 tgacggtcac gaccggcacc ggccggggac cggctgcggg ggccgccggc tggagccgct
     9241 tgggtcgggc caccgcgtag taggcgccgg cggcgatgag gaccgacagg atggcgcaga
     9301 tcacccagaa tcgccgggcc cttcccgcca tgccgcgctc ctcccttggt cgccttgctt
     9361 gtagcatatt ccgacgagcg gccagggcgg atcattcgcg gcggcctcgc gccccgaggc
     9421 tccgggccac gcgctcgggc gtgcccaggt cgcgccaggt caccgccggg atctccgcga
     9481 cggccagcgg gatctcggac gacgcgagga cggcgcgcga gaaatccgcg gtgggcgcga
     9541 agaggtaggc ttgccggagg gccagcgtct cgtaccgcgt gcccgtgaac aggtcgagcc
     9601 gggtgagccg gtcgtggagc agcggcaggc acgcgaggcc ggcctcgacg agcgcggccg
     9661 cggtggccgc gaacacgaag gtgttccaca ggccgccggc gccctgcagg cggcgcgcca
     9721 catccgccga cggcttctcg tggaacgcgc ggacgcgccg caccgcgccg gcggccgtgc
     9781 ggcccaccca ctcgccgggc tcgatccagc cgtagtcggg ctcgggctcg atcgggcgca
     9841 cacccagcag caggagccac gcggggtggt tccgcacata gccgcccgcg gcggcgacgt
     9901 ggctcatgaa cagcgactcc tccgcgatga agtgatcggt ggggaagacc gcgaccacgc
     9961 cgccgggatc gcgggtccgg acccagtgcg cggcgagcag caccgccgcc gcggtgcccc
    10021 ggtcgcaggg ctgggcgagc acggtcaccg cggggtggtc gccgagctcg tcggcgacgt
    10081 agcccgcgtg gctcgcctgg gtcacgacga cggtctgcga aggcgggatc agtcgcgcca
    10141 cccggtcgag cgtggtgcgc agcagggact tcgagccggc gagcgcgcag tactgctttg
    10201 gccgcgcctc gccgtacagc tcgcgggcga gggatcgcag gcggacgcct tctcctccgg
    10261 caaggacgat ggcccagaga tcgcttctcg aatcgagcat ggcgggacct cctcgcgcgc
    10321 cggcggtcgc ccgagacggg ccgcccccgg cgcgctcgat acgcgggtta gttggagagc
    10381 tccacgaagt cgaacgacgt gacgtcgacc cggtagaagc ggctgtccgg gacgcccacg
    10441 tcgaagtagg cgcgcccgtg ggccggcacc gcgtcgccca ccggggcgac cacgctcgcc
    10501 aggtcctggc cgctcgcgtc gatctcatgg acgcgcagcg cgacgtcgac cgcggagtcg
    10561 ccgccgtcgt tgtagacgta gccggtgatc cgggcccggc ccggcgcccc cgggccgacc
    10621 gcccactcga cgtggaagaa atgatcggcg agggcgctcg cgtcatccgg gtcgaaggcc
    10681 ttgaccacga ccgccgactc gtcccagtcc agcccggggt ccctccagtc ggcgcgcgcc
    10741 acgtgcgcgt cgagtccggc cacgccgagc gcggccaccg cgaagctcgc cagcatcacc
    10801 ttgaagatag ggaacatgga cagtcctcct ccgttcgatt gttgggttgt ccgagacgac
    10861 gggtcggagc gggggactac ggagcgggtg gagcgcgggg cagggccgcg gaggcccaga
    10921 gcgcgagcgg ctgccgctcg ggcgtcgggg ccaggcggac gatcaggtgg gcgacgaacc
    10981 ggagaaccgg cggggccggc ggcgtgtggc cggtcccgga ggcgatcagc gtgacgacgt
    11041 cgtcgccgtc ggcgtcgtcg tagatgccct ggatccaggt gggatccggc gggctcgcga
    11101 aggcgaggcc gggcagcgcg acgggcgcga gcagcagcag ggcgacgagc aggcgcttca
    11161 tgctaggggc gcggcggctg catgagctgg cgcatctcct cgcggagctg ggtcagccgc
    11221 tccttggtct gggcggcccg cgcgagctgc tccggcgtga gcacggcgcg gacctcgagc
    11281 gcggcctgcg cgccgtcctg cgcgagctgg tcgcggagct ggccgatgcg cccgagcagc
    11341 ggctgcaggt cggcgggctg gaggggcccg ggcgccagca gcttggcgcc cagctcctgc
    11401 tgggcctgac ggagctgctc gatgagcggg cgcgtggtgg cgcggtgggt ggacaggatc
    11461 ccgcgcacct tcgcctgctg ctcgggcgtg agagaggccg cgcggaccat cagccacagc
    11521 tcgccgtccg tgctgcccat ggacccgcgc atacccccga gcggggcgcc gaagccgggc
    11581 gggggagact gggcgaaggc cgcggtcgcg gtcgaaacga ccaggagcgt ggcggcgagg
    11641 atcgttcgaa tcgtcagcgt gttcgtcatg gttcacctct ggtcagcacc tggtcgagct
    11701 cctgcgccat cacctcgaga ctcacggccg gatcctccgt cggcgcggcg ggcgggtcgc
    11761 cgctgaccga gaacagcgcg gccgagacat cggccagg
//
LOCUS       DASUFA010000157         7624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_838367, whole genome shotgun sequence.
ACCESSION   DASUFA010000157 DASUFA010000000
VERSION     DASUFA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838367"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFX87_05630"
     CDS             complement(<1..1012)
                     /locus_tag="VFX87_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994426.1"
                     /translation="MLRRMPFEVFLGLRYLRARGHRTNLSLFVWIGVGGVFLGVSALI
                     VVLAVMTGFQDGIRDKIISANPHVLIMEAGGRGVADGGALAEQVAPVAGVVSATPFVL
                     QQALFTSPNGGATGGLLRGVDLATPAVRDAIAAQIRFGSLEPVLKGSEPALVLGRELA
                     RSLGVAPGDHVTVISPQGAMTAVGLVPKMRRFLVAGWVEVGMYEYDASLAYTSLASAQ
                     EFAGLGDRVTGVEVKLANPFDAKDVGRTIAARLKGPYWIRDWMDMNRNLFAALQLEKL
                     ALFVIVTIIVLVAAFAIIGHLVLLVAEKRKEIGILKAMGAGAGSIGAVFLVAGMLIGV
                     GGT"
     gene            complement(1012..2577)
                     /gene="lysS"
                     /locus_tag="VFX87_05635"
     CDS             complement(1012..2577)
                     /gene="lysS"
                     /locus_tag="VFX87_05635"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX4994427.1"
                     /translation="MTTSPGLPPDGNEQMRHRREKLETWRARGVNPFGARFPVTHWAG
                     TLQARFKDADEAGLQSAGSVALAGRVMAMRHHGKTCFAVLRDQSGQIQLYARADVLGD
                     VYPAFVDLDVGDFIGVTGELFRTRTNELTVAVKQFEFLAKALRPLPEKWHGLKDVETR
                     YRRRYVDLIVNPQVREAFVMKSRLIAAMRAFFDARGYLEVETPMMQPIPGGAAAKPFV
                     THHNALDMALYLRIAPELYLKRLVVGGLERVYEINRNFRNEGVSTQHNPEFTMLEFYQ
                     AYADYQDLMELTEELFLHLGQTLLGRDELTYQGEAIRLGPPWRRLPFFEGLGEAIGSP
                     VTPETDARALGEAATRRGGFAADRKTQAVLRGEHGPVALWKEIFDTLVEPSLVQPTFV
                     VDFPIELSPLSKQKEGQPRLVDRFELYVCRREIANAYSELNDPIDQRRRFEEQAKERE
                     RGDEEAHWMDEDFVRALEYGLPPTAGEGIGIDRLAMLFTDSASIRDVILFPHLRPERS
                     GAEDGDAGEEAGA"
     gene            complement(2588..3695)
                     /gene="prfB"
                     /locus_tag="VFX87_05640"
     CDS             complement(join(2588..3625,3627..3695))
                     /gene="prfB"
                     /locus_tag="VFX87_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157109750.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX4994428.1"
                     /translation="MLGELKRDVAELDKRLDDLRGHLDLPAKETRLGLIDAEMSSPSF
                     WEDTRKAQALVQERAELARTVTTFKDLAGRAEETRLLWEMATEAGDESVASEIQDSLR
                     QLGAEVEAFELKIILSRPQDKKNAILSIHPGAGGTESQDWAQMLMRMYLRWAERAGFK
                     AEVVDLLAGEEAGIKSATIEMSGEYAYGHLKGETGVHRLIRISPFDASRRRHTSFASV
                     AVIPEVEDVEVVVRDEDLRVDVYRSSGPGGQGVNTADSAVRITHLPSGLVVACQNERS
                     QLRNRDTAMRILKSRLFQIYEQKQKQELADLTGEKKDIAFGSQIRTYTFAPYQIIKDH
                     RTGIEVGNVEAVMDGGLDPFIRAYLTMARPAPSA"
     gene            complement(3689..5233)
                     /gene="lnt"
                     /locus_tag="VFX87_05645"
     CDS             complement(3689..5233)
                     /gene="lnt"
                     /locus_tag="VFX87_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX4994429.1"
                     /translation="MGAEPGASRGGAAARVLVLASAGVLGALAFPRTGWSPLAWVWLV
                     PALVSGLMRPRRAALLDGWLAGTVFYVVLLRWLDHTFLHYSAIPWPLTWLPIAALAAY
                     CGLYLGVVAGGVAWLRGRLGAGPALALAPALWVVGEWLRGWLMGGFPWGLLGYSQHAQ
                     LPVIQIAELGGVYAVSFLIVAVNAALAGLLALGPRRAWPGAAAAMVLLVAALAFGLHA
                     IGRASAGGAGSVEVAVIQPSIEQTIKWDPARHAQIMDIYERLTREAARARPAVVLWPE
                     TATTIFLRGDPVLLERLRRLSEELGTPILVGSIDRRDRPREQFLNSAFLLTGQGITGK
                     YDKIHLVPFGEYVPLASLLSFVKGWAEFISEFGTGDTETVFPLPGAPFGTVICYEIIF
                     PELVRGFVVRGAAFMANITNDAWFGETSGPWQHLGMLPLRAVEHRVAIARAANTGVSA
                     FVEPTGRIGPMLPLLERGVLHRRIPLRDRTTLYTRLGDWLVYACAGLGAAGVALAFFR
                     RSPASC"
     gene            complement(5223..6044)
                     /locus_tag="VFX87_05650"
     CDS             complement(5223..6044)
                     /locus_tag="VFX87_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX4994430.1"
                     /translation="MSRAASSTGSSTASTKSKPPPPDGQAAIRYAVISDVHANLEALT
                     AVLADAAGQGALGVLCLGDLVGYGADPVACVETVGERASAMVAGNHEHAALGLLDLRW
                     FNPWARAAARWTGEQLDDGHRGFLTDLPLIRTMEDATLVHASPRSPEEWDYLVSEEDG
                     LQVFGDFDTRLCFIGHSHLPGVWSVGSGGPEHRGRLDRPEAQVRLDDGRRYLVNVGSV
                     GQPRDRDPRACYAIWDREARSVTIRRVSYDHRAAAAKILAAGLPRALADRLAHGS"
     gene            complement(5977..6393)
                     /gene="nusB"
                     /locus_tag="VFX87_05655"
     CDS             complement(5977..6393)
                     /gene="nusB"
                     /locus_tag="VFX87_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX4994431.1"
                     /translation="MGRRRKARELSLQLLYQMDVQGEGQPEPHFDDFWLRHPVDPEAR
                     EFAEALVRGTKSHEAEIDELIERYAEHWELERMAVVDRNILRQAIFELRWATGVPPKV
                     AINEALEVAKKFSTHESSRFINGILDRIHKEQAPTS"
     gene            complement(6393..6887)
                     /gene="ribH"
                     /locus_tag="VFX87_05660"
     CDS             complement(6393..6887)
                     /gene="ribH"
                     /locus_tag="VFX87_05660"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX4994432.1"
                     /translation="MAGIAPKRRVAPAARGQRFAVVVARFNEPISNKLLDGALAALAG
                     HGVSGDAVEVHWVPGSFELPQAAQALARTRRFAGVVCVGTVIKGATPHFDYVCGVAAH
                     GISRVGLDTGVPVTFGVITALTEEQAWERAGGAVGNRGEEAALAALEMAGWLRSVRGR
                     KGKR"
     gene            complement(6891..>7624)
                     /gene="ribA"
                     /locus_tag="VFX87_05665"
     CDS             complement(6891..>7624)
                     /gene="ribA"
                     /locus_tag="VFX87_05665"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX4994433.1"
                     /translation="GVICEVLDDEGGMARLPELAALGREYHLKSITIKALIEYRIHKE
                     KLVKRAAITRMPTEHGEYQVIAYETTVDDRLPLALVLGDVKTDEPVLVRVHSECLTGD
                     VFGSHRCDCGSQLHKALTIIQHEGRGVLVYMRQEGRGIGLLNKLKAYELQDLGKDTVE
                     ANHALGFRADLRHYGIGAQILVDLGVKNLKILTNNPKKIVGIEGYGLKVVERLPLEVP
                     ATDANRSYLRTKREKLGHLFSSFPD"
BASE COUNT         1124 a         2718 c         2683 g         1099 t
ORIGIN      
        1 ccgtgccccc gaccccgatc agcatcccgg ccaccaggaa cacggcgccg atactccccg
       61 ccccggcccc catcgccttg aggatgccga tctccttccg cttctcggcc accagcagga
      121 ccaggtggcc gatgatggcg aaggccgcca ccaggacgat gatggtgacg atgacgaaca
      181 gcgccagctt ctcgagctgg agcgccgcga acaggttgcg attcatgtcc atccaatcac
      241 ggatccagta gggccccttg aggcgggccg cgatggtccg ccccacgtcc ttggcgtcga
      301 agggattggc gagcttcacc tccacgcccg tgacgcggtc gcccaggccg gcgaactcct
      361 gggccgaggc cagcgacgtg taggcgagcg aggcgtcgta ctcgtacatg ccgacctcga
      421 cccagccggc caccaggaag cgccgcatct tggggacgag ccccaccgcg gtcatcgccc
      481 cctgcgggga gatcaccgtg acgtggtcgc ccggggcgac tccgaggctc cgcgccagct
      541 cccggcccag gacgagggcg ggctccgagc ccttgagcac cggctcgagg ctgccgaagc
      601 gaatctgggc ggcgatcgcg tcccgcaccg ccggggtggc caggtccacc ccgcgcagga
      661 gcccgccggt cgctccgccg ttcggcgagg tgaagagcgc ctgctggagc acgaagggcg
      721 tggcggagac gacgccggcg accggggcca cctgctcggc gagggcgccg ccgtcggcca
      781 ccccgcgccc gcccgcttcc atgatcagca cgtgcgggtt ggccgagatg atcttgtccc
      841 ggatgccgtc ctggaagccg gtcatcaccg cgagcaccac gatgagcgcg ctcacgccca
      901 ggaacacgcc gccgacgccg atccagacga agagcgagag attggtccga tggccgcgcg
      961 cccgcaggta gcggaggccg aggaacacct cgaagggcat gcgtcgcagc atcaggcgcc
     1021 ggcctcctca cccgcgtcgc cgtcctccgc gccgctccgc tcgggccgga ggtgcgggaa
     1081 gaggatcacg tcccggatgg acgccgagtc cgtgaacagc atggcgagcc ggtcgatgcc
     1141 gatcccctcc cccgcggtgg ggggcaggcc gtactccagc gcgcgcacga agtcctcgtc
     1201 catccagtgc gcctcctcgt cgccgcgctc gcgctccttc gcctgctcct cgaaccggcg
     1261 gcgctggtcg atcgggtcgt tgagctcgga gtaggcgttg gcgatctcgc gccggcagac
     1321 gtagagctcg aagcggtcca ccagccgcgg ctgcccctcc ttctgcttcg agagcggcga
     1381 cagctcgatc gggaagtcca ccacgaaggt gggctgcacc agcgagggct cgacgagcgt
     1441 gtcgaagatc tccttccaca gtgcgaccgg gccgtgctcg ccgcggagca ccgcctgggt
     1501 cttgcgatcg gccgcaaacc cgccccggcg cgtcgccgcc tccccgagcg cgcgcgcgtc
     1561 ggtctctggc gtgaccgggc tgccgatcgc ctcgcccagc ccctcgaaga agggcagccg
     1621 gcgccacggc gggcccaggc ggatcgcctc gccctggtac gtgagctcgt cccggcccag
     1681 cagggtctgg cccaggtgga ggaacagctc ctcggtgagc tccatgaggt cctggtagtc
     1741 ggcgtaggcc tggtagaact ccagcatcgt gaactcggga ttgtgctggg tcgacacccc
     1801 ttcgttccgg aagttccggt tgatctcgta cacgcgctcg aggccgccca ccacgaggcg
     1861 cttcagatag agctcggggg cgatgcgcag gtagagcgcc atgtcgagcg cgttgtggtg
     1921 cgtgacgaac ggcttggcgg cggcccctcc cgggatcggc tgcatcatgg gggtctccac
     1981 ctcgaggtag ccgcgcgcgt cgaagaaggc gcgcatggcc gcgatgaggc ggctcttcat
     2041 cacgaaggcc tcgcggacct ggggattgac gatcaggtcc acgtagcgcc gccggtagcg
     2101 cgtctccacg tccttcaggc cgtgccactt ctcgggcagc gggcgcaggg ccttggcgag
     2161 gaactcgaac tgcttcaccg ccaccgtcag ctcgttggtg cgggtgcgga agagctcgcc
     2221 ggtgaccccg atgaagtcgc cgacgtcgag atccacgaag gcggggtaga cgtcgcccag
     2281 cacgtcggca cgcgcgtaga gctggatctg gcccgactgg tcgcgcagca ccgcgaagca
     2341 ggtcttgccg tggtggcgca tcgccatcac ccggccggcc agcgcgaccg agcccgcgct
     2401 ctgcaggccg gcctcgtccg cgtccttgaa gcgcgcctgc agcgtgcccg cccagtgggt
     2461 gaccgggaag cgcgccccga acgggttgac cccgcgggcc cgccaggtct cgagcttctc
     2521 ccggcggtgg cgcatctgct cgttgccgtc gggcggcagg ccgggcgacg tggtcatggg
     2581 cgcgcgatca cgccgagggc gcgggccggg ccatggtgag ataggcgcgg atgaaggggt
     2641 cgagcccgcc gtccatcacc gcttccacgt tgcccacctc gatcccggtc cggtggtcct
     2701 tgatgatctg gtagggcgcg aaggtatacg tgcggatctg gctgccgaag gcgatgtcct
     2761 tcttctcccc ggtcaggtcg gccagctcct gcttctgctt ctgctcgtag atctggaaca
     2821 ggcgcgactt gagaatgcgc atggcggtgt cgcggttgcg gagctgcgag cgctcgttct
     2881 ggcaggccac cacgaggccg ctcggcagat gcgtgatgcg caccgcggag tccgccgtgt
     2941 tcactccctg accgcccggt cccgaggagc ggtagacgtc cacgcggagg tcctcgtcgc
     3001 gcacgaccac ctcgacgtcc tcgacctccg ggatgaccgc gacggacgcg aaggaggtgt
     3061 ggcggcgccg cgaggcgtcg aagggcgaga tgcggatcag gcggtgcacg cccgtctcgc
     3121 ccttcaggtg gccgtaggcg tactccccgc tcatctcgat ggtggccgac ttgatgcccg
     3181 cttcctcgcc cgcgagcagg tccaccacct cggccttgaa gccggcgcgc tccgcccagc
     3241 gcaggtacat gcgcatgagc atctgggccc agtcctgcga ctcggtcccg cccgcccccg
     3301 ggtggatgga caggatggcg ttcttcttgt cctgaggccg cgagaggatg atcttcagct
     3361 cgaaggcctc gacctctgcc ccgagctgcc ggaggctgtc ctggatttcc gaggccacgc
     3421 tctcgtcgcc ggcctcggtg gccatctccc agagcaggcg ggtctcctcc gcgcggcccg
     3481 cgaggtcctt gaaggtggtg acggtgcgcg ccagctcggc gcgctcctgc acgagcgcct
     3541 gtgccttgcg ggtgtcctcc cagaagctgg gcgaggacat ctcggcgtcg atcaggccga
     3601 ggcgggtctc cttggcgggg aggtcaaaga tgccctcgga gatcgtcgag ccgcttgtcg
     3661 agctcggcca cgtcacgctt gagctctcct agcatgacgc gggtgatctc cggaagaagg
     3721 cgagagcaac gccggccgcc cccagacccg cgcaggcata gacgagccag tcgccgagtc
     3781 gcgtgtagag cgtggtgcga tcgcggagcg ggatgcggcg gtggaggacg ccgcgctcga
     3841 gcagcggcag catcggtccg atccgccccg tgggctccac gaacgccgag acaccggtat
     3901 tggcggcgcg cgcgatggcc acgcggtgct cgacggcgcg gagcggtagc atccccaggt
     3961 gctgccaggg gccgctcgtc tccccgaacc acgcgtcgtt ggtgatattg gccatgaacg
     4021 ccgctccgcg gaccacgaat ccccggacca gctcggggaa gatgatctcg tagcagatca
     4081 cggttccgaa cggcgcgccc ggcagcggaa agacggtctc ggtgtcaccg gtgccgaatt
     4141 ccgagatgaa ctccgcccag cccttgacga agctgagcag actcgcgagc ggaacgtact
     4201 ccccgaacgg aaccaggtga atcttatcat acttgcccgt aatcccttgt ccggtcagca
     4261 gaaatgcgct gttcaggaac tgctcccggg gccggtcccg gcggtcgatc gagcccacca
     4321 ggatcggcgt gccgagctcc tccgacagcc ggcgcaggcg ctcgagcagc accgggtcgc
     4381 cgcgcaggaa gatggtggtc gcggtctcag gccacagcac gacggcgggt cgggcgcgtg
     4441 cggcctcgcg ggtgagccgc tcgtagatgt ccatgatctg cgcgtggcgc gccggatccc
     4501 acttgatcgt ctgctcgatg gacggctgga tcaccgcgac ctccaccgag ccggcgcccc
     4561 ccgcgctcgc ccgcccgatg gcgtggaggc cgaatgcgag cgccgcgacg agcaggacca
     4621 tcgccgccgc cgcgcccggc caggcgcggc ggggaccgag ggcgagcagc ccggcgagcg
     4681 ccgcgttcac cgccacgatc aggaaggaca ccgcgtagac gccgcccagc tcggcgatct
     4741 ggatcacggg cagctgcgcg tgctgcgagt agccgagcag gccccagggg aagccgccca
     4801 tgagccagcc ccgcagccac tcgcccacca cccagagcgc gggggccagc gccagggccg
     4861 ggcccgcgcc gaggcggccg cgaagccagg ccaccccgcc cgcgaccacg ccgaggtaga
     4921 ggccgcaata cgcggcgagc gccgcgatgg gcagccaggt cagcggccag gggatcgcgc
     4981 tgtagtggag gaaggtgtgg tcgagccagc gcaggagcac aacgtagaac acggtgccgg
     5041 ccagccagcc gtccagcagg gcggcgcggc gcgggcgcat cagtcccgag accagcgcgg
     5101 ggacgagcca gacccacgcc agcggcgacc agcccgtcct gggaaacgcg agcgcgccca
     5161 ggaccccggc ggatgccaag accaggacgc gggcggcggc gccgcctcgg ctcgcgcccg
     5221 gctcagctcc catgcgcgag tcggtcggcc agcgcgcgcg gcaggcccgc ggcgaggatc
     5281 ttcgcggccg cggcccggtg atcgtaggag acgcgccgga tcgtcaccga gcgggcctcg
     5341 cggtcccaga tcgcgtagca ggcgcgcggg tcgcggtcgc ggggctggcc gacgctgccc
     5401 acgttgacga ggtaccggcg gccgtcgtcg agccgcacct gggcctccgg ccggtcgagc
     5461 cggccgcggt gctccggtcc cccgctgccc accgaccaga ccccgggcag gtgcgagtgg
     5521 ccgatgaagc agagccgcgt gtcgaagtcg ccgaagacct gcaggccgtc ctcctcggag
     5581 acgagatagt cccactcctc cgggctccgc gggctcgcgt ggacgagggt ggcgtcctcc
     5641 atcgtccgga tcagcggcag gtcggtcagg aagccgcgat ggccgtcgtc gagctgctcg
     5701 cccgtccacc gcgcggccgc ccgcgcccac gggttgaacc agcgcaggtc cagcagcccg
     5761 agggcggcgt gctcgtggtt gcccgccacc atcgcgctgg cccgctcgcc caccgtctcc
     5821 acgcacgcca ccgggtcggc tccgtagccg accagatcgc cgaggcagag cacgccgagc
     5881 gcgccctggc cggcggcgtc cgcgagcacg gcggtgaggg cttcgagatt ggcgtggacg
     5941 tccgagatga ccgcgtagcg gatggcggcc tgcccgtcag gaggtggggg cttgctcttt
     6001 gtggatgcgg tcgaggatcc cgttgatgaa gcggctcgac tcatgggtgc tgaacttctt
     6061 cgcgacctcg agcgcctcgt tgatggccac cttgggcggg acgccggtcg cccaccgcag
     6121 ctcgaagatc gcctggcgga ggatgttgcg gtccaccacc gccatgcgct cgagctccca
     6181 gtgctccgcg tagcgctcga tcagctcgtc gatctcggcc tcgtggctct tggtcccgcg
     6241 cacgagggcc tccgcgaact cccgcgcctc gggatcgacc ggatgccgca gccagaagtc
     6301 gtcgaagtgc ggctcgggtt gcccctcgcc ctgaacgtcc atctggtaga ggagctggag
     6361 cgagagctcc cgcgccttgc gccggcggcc catcagcgct tgcccttccg gccccggacc
     6421 gagcgcagcc agcccgccat ctcgagcgcg gccagggcgg cttcctcacc acggttgccc
     6481 acggcgccgc ccgcccgctc ccacgcctgc tcctcggtga gcgcggtgat cacgccgaag
     6541 gtcaccggca cgccggtgtc gagcccgact cggctgatgc cgtgcgcggc caccccgcac
     6601 acgtagtcga agtgcggcgt cgcgcccttg atcaccgtgc ccacgcacac gacgcccgcg
     6661 aagcggcggg tgcgcgcgag cgcctgcgcg gcctgcggca gctcgaagga gccgggcacc
     6721 cagtgcacct ccaccgcatc cccgctgacc ccgtggccgg ccagcgcggc aagggcgccg
     6781 tcgagcagct tgttggagat gggctcgttg aagcgggcca ccacgaccgc gaaccgctga
     6841 ccgcgggcgg cgggggcgac tcggcgcttg ggagcgatac cggccatggg ctagtcggga
     6901 aaggacgaga agagatgccc gagcttctcg cgcttcgtgc ggaggtagct ccggttggcg
     6961 tcggtcgcgg gcacctcgag gggcagccgc tccaccacct tgaggccgta gccctcgatg
     7021 cccacgatct tcttcgggtt attggtgagg atcttgaggt tcttcacgcc gagatccacc
     7081 aggatctgag ccccgatgcc gtagtgccgc agatcggcgc ggaagccgag cgcgtggttg
     7141 gcctccacgg tgtccttgcc cagatcctgt aattcgtagg ccttgagctt gttgagcagg
     7201 ccgatcccgc gcccttcctg ccgcatgtac acgaggacgc cgcgcccctc gtgctggatg
     7261 atcgtcaggg ccttgtgcag ctgggagcca cagtcgcagc gatgggagcc gaagacgtcg
     7321 ccggtgaggc actcggagtg cacgcgcacg agcaccggct cgtcggtctt gacgtcgccc
     7381 agcaccagcg ccagcggcag gcggtcgtcc accgtggtct cgtaggcgat gacctggtac
     7441 tcgccgtgct cggtgggcat gcgcgtgatc gccgcccgct tgaccagctt ctccttgtgg
     7501 atccggtact cgatcagggc cttgatcgtg atggacttga ggtggtactc gcggccgagc
     7561 gccgccagct cgggcaggcg cgccatgccg ccctcgtcgt ccagcacctc gcagatcacg
     7621 ccgg
//
LOCUS       DASUFA010000158        21347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_252180, whole genome shotgun sequence.
ACCESSION   DASUFA010000158 DASUFA010000000
VERSION     DASUFA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 21347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21347
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252180"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1146)
                     /locus_tag="VFX87_05670"
     CDS             complement(349..1146)
                     /locus_tag="VFX87_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4994434.1"
                     /translation="MSESVPRVPVLQVDVAEGVARVTLNRPEVRNALNAALLGELESA
                     LRRLEDDPAARVIVLRGAGDRAFCAGADLKHVGDRGTTLQARDSFGGLARILESMARM
                     RTPVIARVHGYALAGGCGLAAGSDIVIAADDAVFGLPEIKVGLLPLIVMAPILRAVGR
                     KRGMLMILSGAPVSAREALEMGLVSRVVPRAELDTEVDALARTLAGYSPTALGLAKEA
                     AATAPDMEYGAALRYLRELITLVALSDDAREGIAAFFDKRPPRFTGR"
     gene            complement(1143..1850)
                     /locus_tag="VFX87_05675"
     CDS             complement(1143..1850)
                     /locus_tag="VFX87_05675"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX4994435.1"
                     /translation="MAPARPQRRTLRLPPVAAFRLLPVPAAPAPAGLNMAAAPLDLEN
                     LRRLLDAGLSRRPRVALDRSDLVSAAVLVPVTDHDGPHVLFTKKTAHVPHHKGQFSFP
                     GGVVEQRDGSRVETALREAWEEIRLPAEAVEVLGLLDDTETRATNFIITPVVGIVRDH
                     VDFAPDGREIERVLEVPLATLRDPSIFRTEIWERNGEPHPVQFYQVSADDVVWGATAR
                     ILSQFLDLLDEPGASAR"
     gene            complement(1897..2664)
                     /locus_tag="VFX87_05680"
     CDS             complement(1897..2664)
                     /locus_tag="VFX87_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4994436.1"
                     /translation="MTAALVEHLLKGRLGRAAAGPVGELAPDLTLEQAYALQRQLEQA
                     LVGRGECVVGYKVGFTTAALQERHGMTEPVLGFMLASGVFASGDAVPLARFVAIGVEV
                     EVAFLLKADLAGPGVTPASALLAVEGALPSFELIDFRLSGTPRGIDVVADGVYTNAIV
                     LGRPLTPVTGIDLALEGVVLEQDGQIVATQTAAEVLGNPLVSLAWAANTLGRMGRGLH
                     AGDIVLTGSISKVLRPTAGQSVRASFTRLGSVSCRFA"
     gene            complement(2661..3446)
                     /locus_tag="VFX87_05685"
     CDS             complement(2661..3446)
                     /locus_tag="VFX87_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX4994437.1"
                     /translation="MSGSDLERLADLVAGSSRIVAFTGAGVSTESGIPDFRSPGGVWD
                     RFDPSEFTFQNFMAGEAGRRRYWELGRTTFPVIRRAEPNPAHHALVSLWRLGKLDCVI
                     TQNIDNLHQRAGLPADRVIELHGNATRARCLTCERPYDRERVQEWLDAGVAVPLCDPP
                     CGGIIKPRTVMFGEAMPRREMEEAERRARAADHFVVVGSSLVVYPAAYMPLHAKHAGA
                     TLAIVNLTDTPHDGQADLVIRGSAAAVLATVTARLEPRSAARP"
     gene            complement(3443..3802)
                     /locus_tag="VFX87_05690"
     CDS             complement(3443..3802)
                     /locus_tag="VFX87_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994438.1"
                     /translation="MPELTRRYIVMAMATEAPFDVHDPEAPFVLKPWKDPAALRALEA
                     YRDHCYPELAGDLTGWIQVIRSGARIRGGVGARNDAYAGARSKAVRSAPAARRPVKAM
                     KKKVASKTAKHKGKRRG"
     gene            complement(3860..5047)
                     /locus_tag="VFX87_05695"
     CDS             complement(3860..5047)
                     /locus_tag="VFX87_05695"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX4994439.1"
                     /translation="MSGLGGAVAIIGTGTIKFGENFHQSLPDMIYEAVTLALADAGIE
                     RGRLEAAWLGCYEPMLYGYEGNSGTAVADPLNLFPIPVTRVAAYCATGIEAVRNAALA
                     VASGEYDVVLAVGAEKMRDVPSRGSLVAQAVNRGHPVLAKGRTAPGMFALLASRYFKE
                     YGADEEALGAVALKNHLHGSLNPKAHFQKEITAEQHARAPKVAEPLGLFDCCPTTDGA
                     AAAILTRLDLAPKLTDRYTVIRGIAYAVTAGYWNTQFDPSWNFTSFRATREAAKAAYR
                     MAGVTNPAKQIKVAECHDCFTITEIVNYEDLGFFEPGTGWRAVKAGETRLGGSLPVNT
                     SGGLKSCGHPIGSSGIRMINNIHDQLVGRAGRMQVPGADMGLAHNLGGPGSVSAVAVL
                     SQP"
     gene            complement(5102..6382)
                     /locus_tag="VFX87_05700"
     CDS             complement(5102..6382)
                     /locus_tag="VFX87_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX4994440.1"
                     /translation="MITLIAVSFLVLLTLGVPVAFVIGVSGFVGLWWSGQYPLTVVVK
                     QIFEGVDSFVLLAIPLFILAGALMETGGIAVRLVRLAQVLVGWIRGGLSMAVVVAEYI
                     FSGISGSTVADVSAIGSTMIPPMLRAGYRPEQAVAVVSAASAMGILVPPCILMIVIGS
                     VANVSVAALFAAGFVPAVVLALAIMVYIWFDARRSGIEARERVTWGAVGRAFTDAVIP
                     LGLPAIIFGGILGGVVTPTEAAVLAVVYAFIVGVFVYREIAWKDLPRILVHSAVVTAA
                     VCFLLGTAAVVAWVLAVEQIPQILLRAMLAVPGGNIVFLVLTAVLFILLGAVLEGLPA
                     VVILLPTFMPVVTKLGIDVIHYSTVVVAATGIGLFLPPIGVGFFIACGIANVPSDRAT
                     PAMMPYVYMMCVGLLVVILVPWFTLILPRLLKLG"
     gene            complement(6379..6855)
                     /locus_tag="VFX87_05705"
     CDS             complement(6379..6855)
                     /locus_tag="VFX87_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX4994441.1"
                     /translation="MLARAGANVVEGVLLLMMAVLCADVFLGVFSRYVIRSTFTWYDE
                     IARLLFVWIVFLGAAVGVRRSAHFRLHLLVDRFPPPARRVAHVFGVLVLMGFGLVLIQ
                     QGWKLVELGQFQRTPVMGLSKQYVYASVPAGGALIILYSLPHLWRAFRGESGPAAA"
     gene            complement(6859..7872)
                     /locus_tag="VFX87_05710"
     CDS             complement(6859..7872)
                     /locus_tag="VFX87_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX4994442.1"
                     /translation="MHRCLALVVAVCLAAVLGTPSAAPAQPVLTWKAGTATAGGNPLN
                     DAMEIFAKQMAERSRGRIKVDVHIANALAKGEGAHLEGVQLGTIDVAAIGSAPVGGMF
                     EPSYQALDLPFLWATREQVWKVMDGPLGQELLKKMEAKNLKAFCFGGGWGFRNMMSNK
                     RAIITPEDMKGQTIRVQESPTYVAMMKAMGANPVPMPYVEVYLAMKQGTIDGMELPSF
                     TLTSDKFHEVTKYYSLTRHSYPPIAWFMNLKKYQALAPDLQKAADESMREACQAHRRL
                     EVEKEKLDFEIMKKAGVQINEVKDIKAFQALMKPAYDYVEGQVGKEFMAKLRAAVQAA
                     ATK"
     gene            8056..8595
                     /locus_tag="VFX87_05715"
     CDS             8056..8595
                     /locus_tag="VFX87_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994443.1"
                     /translation="MREPALDGLHARLERLEREIGWWRRVGVAALAGVGLFGAVAATV
                     TTNPDEVKTRRLVITDGEGRGRAVLTVDESDRTRLSLTDRDGATSADLTVTPGQSAAL
                     SVGRPGAQAQLAAAGETGQLSVAARGQRAWLVAAPTGSSLALGNDPGKPQITVATSPT
                     LSPSLQLSDREGNAVWKAP"
     gene            8737..10491
                     /locus_tag="VFX87_05720"
     CDS             8737..10491
                     /locus_tag="VFX87_05720"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX4994444.1"
                     /translation="MTKGSINREVALAGQQVRRKVIFALTLCSVIPLLLLTYAFHAPV
                     REFLGPIVGLKDSVSVPALLAFTVLLMAAGAFIVWDIATALSRAAQLSNEQSPDLPEE
                     PSRKDEIGTLMSSFARMLATIEQQSQEINEFPRRLDQLIREAFRDSLTGLPNRALFMD
                     RLTHALTRAERAGTNLAVLFLDLDRFKTLNDTLGQEVGDRLLVEVGHRLAGCLSPEDT
                     VARLGGDEFALLLEDASDLSGATALAEKVSAEIQRPFVVDGRDVLISASIGVALTGGG
                     SMQPEEVLHNADLAMYQAKAEGRARYELYEPGLSVSTRERLDLQADLRTAGARQELSL
                     RFQPVVALGTMRPLEVEALVRWDHRRRGALLPADFIGLSEETGLILPMGRWILREACR
                     QARAWQAESSGVPPLIVSVNLSAGQFERDALPEEVATIVRETGFEPRRLQLEISEAVL
                     MRDDPRMFDRLDALKAIGVRLAIDDFGTGYASLSYLKRLPVDCLKIDRSLVKGVGYET
                     EDTAVIRAVVSLAHSLGITVTAEGIETQDQLVQLRAVGCDQGQGYFFARPVSADRVPE
                     LLASLGADERNGSPSWLG"
     gene            10479..13445
                     /locus_tag="VFX87_05725"
     CDS             10479..13445
                     /locus_tag="VFX87_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4994445.1"
                     /translation="MARLSLRGLARTLVVRLIGIAAFTGALGLGLSWLLEMAVSEPEW
                     TETAAVAQRSVNRSGLPELLAGQNARSPKRWAEESARFVRELPGVVNIKVWDPQGTVV
                     WAVQSGFVGQRSDGHELRDAISGRVAVRFSSVAPPNAEAQSFVSPNVADLFVPVYGPG
                     AARVVGVVELSQAPLRMDQAHERWSLLAWNIALGTGGILCLILLPAAWRSHRRTLAET
                     SSTSQRSVQRTQELQRTTEQLRDALKAVKQRAEETDRMLEVTEAIGSAIEQKALFEVI
                     AHGAARACRVDRGSIFLRDKSGQLMVPVSSQLADRAGAARGADGAETLRALALEEMPS
                     FLLEAVRRQEPVVISDPGSDPRVPQGWVGLFQAKSALVVPLIHQGRTAGVLILDHLKT
                     LHVFTEEEIRLATTLAAQAAVALEKARLYDEIQQRLQQTETLLAVSQAIGSAPDLPEA
                     IRRTTREMVRILGADTGVAWCMTGGGDRFIPLAGYHVPRDLREPLSGLPLAPDDPLID
                     LVKRLRTALHSSNSKTDPRFNQAALQLLPQASLLIQPVQVSDELLGIFAIAWVREAHV
                     FTMDDLRLADGIAKQAAVAIELHRTQRHIIEQERLNAVGKMASGLAHDFNNALVPISG
                     YAEMLLEHPEILQDAAKSTRYLKLIMTGVEDAASVVSRLREFYRKREEGETYRPLSLN
                     QMVEQAVALTRPRWRDEALGSGRTILVEADMRPVPRILGSESEMRELLANLIFNAVDA
                     MPEGGLIRIRTGMREASASEETPLAPRVPQVFLEVSDTGTGMTEDVRRRCLEPFFSTK
                     GERGTGLGLPMVYGIVKRHRGTMDIESTVGKGTTFIVLLPADTSLAREVAPRAQAPSS
                     GSLHVLVVDDEPIARDVLSEYLMGDGHTVDTAVNGREAFEKFKAGRYALVITDRAMPE
                     VGGDQLAAMVKEVAPDTPVILLTGFGDLMNAAGEKPDGVDMVVKKPIRLATLREVLSK
                     MTDPTRAAETAPSPTA"
     gene            complement(13452..14627)
                     /gene="chrA"
                     /locus_tag="VFX87_05730"
     CDS             complement(13452..14627)
                     /gene="chrA"
                     /locus_tag="VFX87_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX4994446.1"
                     /translation="MGPIFAVFLRLGLTSFGGPIAHLGYYRTEFVERRRWLSEAAYAD
                     LVALCQLLPGPASSQVSFAVGLSRGGLAGGLVAWLGFTLPSAVAMVLFAQGAAALTGP
                     LSTGMLHGLQVVAVAVVAQAVWSMARSLCPDRPRATIAVVATLVVTAAPTAIGQIAAI
                     LAGAAAGAWLCRQPGAIVASERLAVPIPRALAVAALALFALLLIGLPIAAAVTPVPAL
                     AVAEAFYRAGAFVFGGGHVVLPLLHASVVEPGWVSEDAFLSGYGAAQAVPGPLFTFAA
                     YLGAIMTVPPAGWPGAALCLVAIFLPGLLLVLGVLPFLVTLGRVALMPASLRGVNAAV
                     VGVLLGALYDPVWTKGIGGPADFALALVAFALLVVWKAAPWLVVLISAAGGAVVFGR"
     gene            complement(14635..16026)
                     /gene="fumC"
                     /locus_tag="VFX87_05735"
     CDS             complement(14635..16026)
                     /gene="fumC"
                     /locus_tag="VFX87_05735"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX4994447.1"
                     /translation="MTTTRTEKDTMGPIEVPGDRYWGAQTQRSLEHFRIGRDHFPREM
                     IRALGILKKASAFVNRELGLLPEDKARAIVQAAEEVIEGNLDAHFPLVVWQTGSGTQT
                     NMNANEVIANRANELLGGRLGAQQPVHPNDDVNRSQSSNDTFPTAMHIAAVEQIEGRL
                     IPAVGGLRDTLQAKARALDDIVKIGRTHLQDAVPLTLGQEISGWASQLDHGLEHVRAA
                     LPHLRELALGGTAVGTGLNAPRGFGERAARVIADLTGRAFVSAPNKFEALAAHDALVN
                     AHGTLRTLAGALTKIANDVRWLASGPRSGIGELRIPENEPGSSIMPGKVNPTQCEALT
                     MVCAQVMGNDVAVGIGGASGNFELNVFKPLIIHNVLGSVRLLADACDSFDRYCARGIE
                     PDRERIRALVERSLMLVTALAPIIGYDRAAAIAKKAHAEGLTLREATLALGHLSAEEF
                     DRAVRPETMVDPG"
     gene            16098..17093
                     /locus_tag="VFX87_05740"
     CDS             16098..17093
                     /locus_tag="VFX87_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4994448.1"
                     /translation="MDCCGHEGPGGDARFDFRRVADGVHVAVAAPAYKVNSNTAIIES
                     DDGAVIVDTHSKPSAARVIVDRLRDITARPVRYVVNTHFHWDHWHGNEVYPAAYPGAE
                     IITNQLTREAMVKKGFKRIQDHVRQVPAEIARLRAELAAATGPEPRAKLQADLGLAES
                     YLAEVRAMKPALPTMAFERTMTLYRRDREIQLLYLGRAHTEGDVFVYLPKEKVVITGD
                     AVIGWTPFMGDGYPEDWVTTLDRLAELPWDTLVMGHGEPAGRDWLRTFRGYIHDMVEA
                     VREEAATGASLDEVKQRVTAHLAPTYEPAFAAYQHYRPWRQGLLANIERTYPLVS"
     gene            17101..18303
                     /locus_tag="VFX87_05745"
     CDS             17101..18303
                     /locus_tag="VFX87_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4994449.1"
                     /translation="MATDEAIFNPLTPEFHANPYPFYQRLRETDPVHLSPLGLWVLTR
                     YDDCVTSLRDPRFGRDGFEAILSAQYGEESETGRLPRSMLFRDPPDHTRLRSLVNRAF
                     TPRVIEGMRGQIQAVVDRLLDQVEGRGRMDVIADLAYPLPVTVICDMLGVPVGDHEQM
                     RDWSSDIIRSLDAIGIPSDESVVERGRVGRRGIAEYFRALLPERRRHPRADLLSSLIA
                     VEEQGDRLTEGELLATCVLLFIAGHETTVNLIGNGLLALLRHPAELERLRVEPGLIGS
                     AVEELLRFDSPVQRTARITNAEVEVGGKTLPPGAFVVTAIGAANRDPRYFPDPDRLDI
                     GRSENRHIAFGFGIHFCLGAPLARVEGQLALGTLMRRMPKLRLASPDLEWRESSTLRG
                     LKALPVEL"
     gene            complement(18323..18943)
                     /locus_tag="VFX87_05750"
     CDS             complement(18323..18943)
                     /locus_tag="VFX87_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994450.1"
                     /translation="MRGVEQIPWLYDAVCALCEATGLARWRSWLVSGARGRVLDLGCG
                     TGRNLPLLPAGTRTVGLEPSWAVLQRARRRAPRVPLVVGSAEALPFRDAAFDTVVSGL
                     VFCSVRDPHQGLLEVRRVLRPGGQLRMLEHVRATRAWKARLQDLLQPAWTQITGGCHP
                     NRDTEGAVEAGGFQIEEEGRRAKGDMRRFVARPIVPPGTHRDGSGV"
     gene            complement(18952..19821)
                     /locus_tag="VFX87_05755"
     CDS             complement(18952..19821)
                     /locus_tag="VFX87_05755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4994451.1"
                     /translation="MHIGVVFPQVEIGADPGAIRDYAQAVEAMGYTHILTFDHVLGAN
                     PDRPGGWKGPYTYRHSFHEPFVLFGFLAAATRRVELATGILILPQRQTTLVAKQAAAV
                     DVLSSGRLRLGVGVGWNPVEFEALGERFTNRGARIEEQIAVLRALWARDLVTIAGQWH
                     RVTDAGLNPLPPRPTIPIWMGGESEPVVRRAARLADGWMPHFRPGPEAQAIVDRLHGL
                     IREAGRDPADFGIEGRITLAQVPPERRAAELTAWRAMRGITHLCVHTVGLGLKTPAEH
                     VQALELFKKDLLT"
     gene            complement(19831..20412)
                     /locus_tag="VFX87_05760"
     CDS             complement(19831..20412)
                     /locus_tag="VFX87_05760"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4994452.1"
                     /translation="MATGLETGFALFDTAIGPCGIAWNGRGVVGVQLPEGGEAETRAR
                     LRRRFPDTREAPPPAPVRRAIAGVVALLRGEPSELDTVTLDMDGVPPFHRRVYEVART
                     IPPGTTLSYGEVAARMGAPGAARDVGQALGQNPFAIIVPCHRVLAANGKVGGFSARGG
                     VRTKLRLISSERAGGHGALPLFGGAPEDRPPSC"
     gene            complement(20397..20846)
                     /locus_tag="VFX87_05765"
     CDS             complement(20397..20846)
                     /locus_tag="VFX87_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX4994453.1"
                     /translation="MSDKATLLRDADLAFGELRGAIDGLTDEDMRRVWLGTWGVREIL
                     IHISGWHDEMATALGRVAKGDAPYPKGTYDDFDAWNARFVDQKAGVKTADVVADLEAS
                     HRKLLAVAAALPEALFAAGGAARGPFDGAGAGHYREHAEQIRQWRQG"
     gene            20973..>21347
                     /locus_tag="VFX87_05770"
     CDS             20973..>21347
                     /locus_tag="VFX87_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4994454.1"
                     /translation="MAIRIERSDVVATIVMDRPEARNAVDGPAARALIEAFEEFERDE
                     ALRVAVLWGAGGTFCAGADLKARGTANGLTLSPEGPGPMGPTRMAFTKPTIAAVEGHA
                     VAGGLELSLMCDLRVAGDTAVFG"
BASE COUNT         3179 a         7847 c         7446 g         2875 t
ORIGIN      
        1 tgtggagctc cgcgggccct ccgagggtca cgacctgggc cggtagctcc ttcggctggg
       61 cccacaccgg accggcggtg agcccgacga ggagaccggc accgcccacc acggccgccc
      121 acatgatctt gatcacgctg agacctccgg ggacccgggc tgatatcccg gacagcgtag
      181 cacgggcaga cgcggatact tcgcaaacgc cgggtcgcgg gcggcgcgcg cgcagagcca
      241 gaactccgac gcgcgcgccg acacgacgtg gcgcgcctcc gcgcaggcgg cgcagaggcc
      301 ggggtcccgg gactcgccgc gcccgccggc ttgcggcgac ggcattggct agcgcccggt
      361 gaaacgcggg gggcgcttgt cgaagaaggc ggcgatgccc tcgcgggcgt cgtcggagag
      421 cgccaccagg gtgatgagct cgcgcagata gcggagggcc gcgccgtact ccatgtccgg
      481 cgcggtggcc gcggcctcct tggccagccc gagcgcggtc ggcgagtacc cggccagagt
      541 ccgcgccagc gcgtcgacct ccgtgtccaa ctccgcgcgc ggcacgaccc ggctcaccag
      601 gcccatctcg agcgcctccc gcgccgagac cggcgcgccg ctcaggatca tgagcatccc
      661 gcgcttgcgc ccgaccgcgc ggaggatcgg cgccatcacg atcagcggga gcagtccgac
      721 cttgatctcg ggcagcccga agaccgcgtc gtccgccgcg atcacgatgt ccgaaccggc
      781 ggcgagcccg cagccgccgg ccagcgcgta accgtggacc cgggcgatga ccggcgtccg
      841 catccgcgcc atggactcga ggatgcgcgc gaggccgccg aaggagtccc gcgcctgcag
      901 cgtggtgccg cggtcgccga cgtgcttgag atcggcgccc gcgcagaacg cccggtcccc
      961 cgccccgcgc aggacgatca cgcgcgcggc cggatcgtcc tcgagccggc gcagcgccga
     1021 ctcgagctcg ccgaggaggg ccgcattgag cgcgttccgc acctccggcc ggttgagcgt
     1081 gacgcgggcc acaccctccg ccacgtccac ctgcaagacg ggcacccgcg gcaccgactc
     1141 gctcaccggg ccgacgcccc gggctcgtcc agcagatcga ggaactggct caggatgcgg
     1201 gcggtcgcgc cccagaccac gtcgtccgcc gagacctggt agaactgcac cgggtgcggc
     1261 tcgccgttgc gctcccagat ctcggtgcgg aagatagacg ggtcgcgcag cgtggccagc
     1321 ggcacttcga ggacgcgctc gatctcccgc ccatccgggg cgaagtccac gtggtcccgg
     1381 acgatgccga cgaccggcgt gatgatgaag ttggtggcgc gcgtctccgt gtcgtcgagc
     1441 agcccgagca cctccaccgc ctcggcgggc agacggatct cctcccacgc ctcgcgcagc
     1501 gcggtctcca cccgcgagcc gtcccgctgc tcgacgacgc cgcccggaaa cgagaactgc
     1561 cccttgtgat gcggcacgtg cgccgtcttc ttggtgaaga gcacgtgcgg cccatcgtgg
     1621 tccgtcaccg gcaccagcac cgccgccgac accagatcgc tccggtccag cgccacccgc
     1681 ggccgccggc tcagccccgc atccagaagc cgccggaggt tctcgaggtc gagcggcgcg
     1741 gccgccatgt tcaggccggc aggagccgga gctgccggaa ccggcaggag acggaacgcg
     1801 gcgaccggcg ggagacggag cgtccgccgc tgggggcgtg cgggggccat gtccgggccc
     1861 ccgcttctgg agagaagctt ccggtcgccc gccggatcac gcgaagcgac aggagaccga
     1921 ccccagccgc gtgaacgatg cgcgcaccga ctgccccgcc gtgggccgca gcactttcga
     1981 gatcgagccg gtgagcacga tgtcgcccgc gtggaggcca cggcccatcc ggcccagcgt
     2041 gttcgcggcc cacgcgaggg agacgagcgg gttgcccagc acctcggccg cggtttgggt
     2101 ggcgacgatc tggccgtcct gctcgaggac cacgccctcg agcgccaggt cgatgccggt
     2161 caccggggtg agcggacggc cgagcacgat cgcgttcgtg tagacgccgt cggcgacgac
     2221 gtcgatcccg cgcggcgtgc cgctcagccg gaagtcgatc agctcgaagg agggcagcgc
     2281 gccttccact gccagcaggg cggacgcggg ggtgacgccg ggaccggcca ggtccgcctt
     2341 gagcaggaac gcgacctcga cctcgacccc gatggcgacg aagcgcgcga gcggcaccgc
     2401 gtctccgctg gcgaagaccc cggaggcgag catgaagccc agcaccggct cggtcatgcc
     2461 gtgacgctcc tggagcgcgg cggtggtgaa gcccaccttg tagcccacca cgcattcccc
     2521 gcggccgacc agtgcctgct cgagctggcg ctgcagcgcg taggcctgct cgagggtcag
     2581 gtccggcgcg agctcaccga ccgggcccgc ggcggcgcga cccagccgtc ccttcaggag
     2641 atgctcgacg agcgccgcgg tcatggccgc gcggccgacc gcggctcgag ccgggcggtg
     2701 accgtggcga gcacggcggc cgcgctgccc cggatcacga ggtcggcctg gccgtcgtgg
     2761 ggcgtgtcgg tcaggttcac gatggccagg gtggcgcccg cgtgcttggc gtggagcggc
     2821 atgtaggccg ccgggtagac gaccagggac gagcccacga ccacgaagtg gtcggcggcc
     2881 cgcgcgcgcc gctccgcctc ttccatctcg cgccggggca tggcctcgcc gaacatgacg
     2941 gtgcggggct tgatgatccc gccgcagggg ggatcgcaga gcggcaccgc cacgcccgcg
     3001 tccagccact cctgcacgcg ctcgcgatca taggggcgct cgcacgtgag acaccgggct
     3061 cgggtggcgt tgccgtgcag ctcgatcacc cggtccgcag gcaggccggc ccgctggtgg
     3121 aggttgtcga tgttctgggt gatcacgcag tcgagcttcc cgagccgcca caggctcacc
     3181 agcgcgtgat gcgccgggtt gggctcggcg cggcggatca ccgggaaggt ggtgcggccc
     3241 agctcccagt agcggcggcg gcccgcctcg cccgccatga agttctggaa ggtgaactcg
     3301 ctgggatcga agcggtccca gacgccgccc gggctgcgga agtccgggat cccggactcg
     3361 gtgctgacgc cggccccggt gaaggcgacg atgcgcgagg acccggcgac gaggtctgcg
     3421 agccgctcga gatcggatcc gctcacccgc gccgctttcc tttgtgtttg gctgttttgc
     3481 tggccacttt cttcttcatg gctttaaccg gccggcgcgc ggccggcgca gagcgaaccg
     3541 ccttcgaccg cgcacccgca tacgcatcgt tccgggcgcc caccccgccg cggatccggg
     3601 cgccgctccg gatgacctgg atccacccgg tgaggtcgcc ggccagctcc gggtagcagt
     3661 ggtcgcggta ggcttcgagc gcgcgcaggg ccgccggatc cttccacggc ttgagcacga
     3721 acggcgcctc ggggtcgtgc acgtcgaacg gcgcttcggt tgccatggcc atgacgatgt
     3781 accgccgggt cagctcgggc atgtgggcct cctggcctgg cggggggcct cgcccggccc
     3841 cgccccgccg gccacgctgt cagggctgag agagcaccgc gacggccgag acagaccccg
     3901 gaccgcccag gttgtgcgcc agccccatgt cggcgcccgg cacctgcatg cggccggcgc
     3961 gcccgacgag ctggtcgtga atgttgttga tcatccggat gcccgacgag ccgatcggat
     4021 gcccgcacga cttgagcccg ccggatgtat tgacgggcag cgacccgccg agccgggtct
     4081 cccccgcctt caccgcccgc cagcccgtgc ccggctcgaa gaagcccagg tcctcatagt
     4141 tcacgatctc ggtgatggtg aagcagtcgt ggcactcggc gaccttgatc tgcttcgcgg
     4201 gattggtgac cccggccatc cggtaggccg ccttcgccgc ctcccgggtc gcccggaacg
     4261 acgtgaagtt ccaggagggg tcgaactgag tgttccagta gcctgcggtc accgcgtagg
     4321 cgatgccgcg gatcacggtg tagcgatcgg tgagcttcgg ggcgaggtcc aagcgggtga
     4381 ggatcgcggc cgccgcgccg tccgtcgtcg gacagcagtc gaagagcccg agcggctcgg
     4441 ccaccttcgg ggcacgggcg tgctgctccg cggtgatctc cttctggaaa tgcgccttcg
     4501 ggttgagcga gccgtggagg tggttcttga gcgcgaccgc gcccagcgcc tcctcgtccg
     4561 cgccgtactc cttgaagtag cgcgaggcga gcagggcgaa catcccgggc gcggtgcgcc
     4621 ccttggcgag caccgggtgc ccgcggttga ccgcctgggc caccagcgag ccccgcgagg
     4681 gcacgtcgcg catcttctcg gcgccgaccg ccaggaccac atcgtactcg ccggaggcta
     4741 ccgccagcgc cgcgttccgc accgcctcga tgccggtggc gcagtaggcg gccacgcgcg
     4801 tgaccgggat cgggaagagg ttgagcgggt ccgcgaccgc ggtgcccgag ttgccctcgt
     4861 agccatagag catgggctcg tagcagccga gccaggccgc ctcgagccgg ccgcgctcga
     4921 tgcccgcgtc cgccagggcg agcgtcaccg cctcgtagat catgtcgggg aggctctggt
     4981 ggaagttctc cccgaacttg atcgtgccgg tgccgatgat cgcgaccgcg ccgccgagtc
     5041 cgctcatggc tgtctccttt cgccgcccta gcctactctg gcgcgggcgc gggccgcaag
     5101 atcagccgag cttcagcagc cgcgggagaa tcagggtgaa ccacgggacc aggatgacga
     5161 cgagcaggcc cacgcacatc atgtagacgt agggcatcat cgcgggggtc gcgcggtcgc
     5221 tgggcacgtt ggcgatgccg cacgcgatga agaagccgac cccgatcggg ggcaggaaga
     5281 ggccgatgcc ggtggccgcc accaccaccg tgctgtagtg gatgacgtcg atcccgagct
     5341 tggtcaccac cggcatgaag gtgggcagca ggatcaccac cgcgggcagg ccctcgagca
     5401 ccgcgcccag caggatgaag agcaccgcgg tgagcacgag gaagacgatg ttcccgccgg
     5461 gcaccgccag catcgcgcgg aggaggatct gcgggatctg ctccaccgcc agcacccagg
     5521 ccaccaccgc ggcggtcccg agcaggaagc acaccgcggc ggtcaccacc gcgctgtgca
     5581 ccaggatgcg cggcaggtcc ttccaggcga tctcgcgata gacgaagacc cccacgatga
     5641 acgcgtagac caccgcgagc accgccgcct cggtcggcgt caccaccccg ccgaggatgc
     5701 cgccgaagat gatggcgggc agcccgagcg ggatcaccgc gtcggtgaac gcgcggccca
     5761 ccgcgcccca ggtgacgcgc tcccgcgcct cgatgccgct gcggcgggcg tcgaaccaga
     5821 tgtagaccat gatcgccagc gcgaggacca ccgcgggcac gaagcccgcc gcgaacagcg
     5881 cggccaccga cacgttggcg accgagccga tcacgatcat caggatgcag ggtggcacca
     5941 ggatccccat cgcggaggcc gcggagacca ccgctaccgc ctgctccggc cggtagccgg
     6001 cccgtagcat cggcgggatc atggtggagc cgatggccga gacgtcggcc accgtggagc
     6061 ccgagattcc ggagaagatg tactccgcca ccaccaccgc catcgagagg ccgccgcgga
     6121 tccaccccac cagcacctgg gccagccgca cgagacggac cgcgataccc ccggtctcca
     6181 tcagggcgcc cgccaggatg aagaggggga tggcgagcag cacgaacgaa tccacgccct
     6241 cgaagatctg cttcacgacc acggtgagcg ggtattgccc gctccaccag aggcccacga
     6301 agcccgagac gccgatgacg aaggccaccg gcacccccag cgtgagcagg acgaggaagg
     6361 agaccgcgat cagcgtgatc atgccgcggc gggtccgctc tcgccgcgga aggcgcgcca
     6421 gaggtgcggc aggctgtaga gaatgatcag ggccccgccc gcgggcaccg aggcgtacac
     6481 gtactgcttg gagaggccca tcacgggcgt gcgctggaac tgcccgagct ccaccagctt
     6541 ccagccctgc tggatcagga ccaggccgaa gcccatcagg acgagcaccc cgaagacgtg
     6601 ggcgacgcgc cgggccgggg gcgggaagcg gtcgaccagg agatgcaggc ggaagtgggc
     6661 gctgcgccgc acgcccaccg ccgcccccag gaacacgatc cacacgaaga gcagccgggc
     6721 gatctcgtcg taccacgtga aggtgctgcg gatcacgtag cgggagaaga cgcccaggaa
     6781 gacgtccgcg cacagcaccg ccatcatcag gaggagcacg ccctccacga cgttcgcccc
     6841 ggcgcgggcc agcacggact acttggttgc cgccgcctgc accgccgccc ggagcttggc
     6901 catgaactcc ttgcccacct gcccctcgac atagtcgtag gccggcttca tgagcgcctg
     6961 gaaggccttg atgtccttca cctcgttgat ctgcacgccc gccttcttca tgatctcgaa
     7021 gtcgagcttc tccttctcga cctcgagacg gcggtgggcc tggcacgcct cgcgcatcga
     7081 ctcgtccgcc gccttctgga gatccggcgc cagcgcctgg tacttcttga ggttcatgaa
     7141 ccaggcgatc ggcgggtagg agtggcgggt gagcgagtag tacttggtca cctcgtggaa
     7201 cttgtccgag gtcagcgtga aggacggcag ctccatgccg tcgatcgtgc cctgcttcat
     7261 cgccaggtac acctcgacgt agggcatggg caccgggttg gcccccatcg ccttcatcat
     7321 ggccacgtag gtgggcgact cctgcacccg gatcgtttgc cccttcatgt cctccggcgt
     7381 gatgatggcg cgcttgttcg acatcatgtt gcggaagccc caaccgccgc cgaagcagaa
     7441 cgccttgagg ttcttggcct ccatcttctt gagcagctcc tggccgagcg gcccgtccat
     7501 caccttccac acctgctcgc gggtggccca gaggaacggc aggtcgaggg cctggtagga
     7561 gggctcgaac ataccgccca ccggcgcgct gccgatcgcc gccacgtcga tcgtgccgag
     7621 ctgcacgccc tccaggtgcg cgccctcgcc cttggccagc gcgttggcga tgtgcacgtc
     7681 gaccttaatc cgcccgcggc ttcgctcggc catctgcttc gcgaagatct ccatcgcgtc
     7741 attcagcgga ttgccgccgg cggtcgccgt gcccgccttc cacgtcagga cgggttgcgc
     7801 gggggcggcg gacggcgtgc ccaggacggc ggccaggcag acggcgacga caagagccag
     7861 gcaacgatgc atggttggat ctcctcggag tcggtcgggc acggcaactg gaagcggggg
     7921 cgtcatcgga agccgctctt gtgtaacatg ccggatcgag gggggtcaag gtgggcgggc
     7981 cagtcgatgc ccgccggccg cggcccggac gagacgagaa atccaccacc gagaaccgca
     8041 gggggaggaa gcgcgatgag ggagccggca ctcgatggac tgcacgcacg gttggagcgc
     8101 ctcgagcgcg agatcggctg gtggcgccgc gtgggcgtcg ccgcgctcgc cggcgtgggc
     8161 ctcttcggcg ccgtcgcggc caccgtcacc accaatcccg acgaggtgaa gacccggcgg
     8221 ctcgtgatca ccgacggcga gggccgcggc cgcgccgtct tgaccgtgga cgagagcgac
     8281 cgcacgcgcc tgtcgctcac ggaccgcgac ggcgccacca gcgcggatct gaccgtcacc
     8341 ccggggcagt ccgcggccct ctccgtcggc cgcccggggg cccaagccca gctcgcggcc
     8401 gcgggcgaga ccggccagct ctcggtcgcg gcccgggggc agcgggcgtg gctcgtcgcg
     8461 gccccgaccg ggtcgagcct ggcgctcggc aacgacccgg gcaagcccca gatcacggtc
     8521 gccaccagcc cgaccctgag ccccagcctt cagctttccg atcgagaggg caacgccgtc
     8581 tggaaggcgc cgtagacgaa aatcgtcgtc cgcgtggacg ttttttaagg ggcggcgtac
     8641 tgcggaccct cgtgttatca accgcttagc ctccgctatt cgtggcaccg ctcttgctcg
     8701 atttcacacc gcctggagtc ctgccaggtg acacgaatga ccaagggctc gatcaatcgc
     8761 gaggtggccc tcgccggcca gcaagtccgg cgcaaggtga tcttcgccct caccctgtgt
     8821 tcggtcatcc cgttgctcct cctgacgtac gcgttccatg ccccggtgcg cgagttcctg
     8881 ggcccgatcg tggggctgaa ggactcggtg agcgtcccgg cgctgctcgc cttcaccgtg
     8941 ctcctcatgg ccgccggcgc gttcatcgtc tgggacatcg ccaccgccct gtcgcgcgcc
     9001 gcgcagctca gcaacgagca gagcccggac ttgcccgagg agccttcccg caaggacgag
     9061 atcggcaccc tcatgagctc cttcgcgcgc atgctcgcca ccatcgagca gcagtcgcag
     9121 gagatcaacg agttcccgcg ccggctggac cagctcatac gcgaggcctt ccgcgactcc
     9181 ctgaccgggc tgcccaatcg tgccctgttc atggatcgcc tgacccacgc gctgacccgc
     9241 gcggagcgcg ccggcaccaa cctggcggtc ctcttcctcg acctggaccg cttcaagacc
     9301 ctgaacgaca cgctcggcca ggaggtcggc gaccgactcc tggtggaagt cgggcatcgc
     9361 ctggccgggt gcctgagccc cgaggacacc gtcgcgcgcc tgggcggaga cgagttcgcg
     9421 ctgctgctgg aggacgcctc cgatctcagc ggcgccaccg ccctggccga gaaggtctcc
     9481 gcggagatcc agcggccgtt cgtcgtggac gggcgcgacg tcctgatctc ggcgagcatc
     9541 ggggtcgccc tcaccggcgg tggatccatg cagccggagg aagtgctcca caacgccgac
     9601 ctggccatgt atcaggccaa ggccgagggc cgcgcgcggt acgagctcta cgagccgggc
     9661 ctcagcgtct cgacccgcga gcgcctggac ctgcaggccg acctccgcac cgccggagcg
     9721 cgccaggagc tgagcctgcg cttccagccg gtggtcgcgc tgggcacgat gcgcccgctc
     9781 gaggtcgagg ccctggtccg ctgggatcac cgccggcgcg gggcactgct ccccgccgac
     9841 ttcatcggcc tgtcggaaga gaccggcctg atcctgccga tgggccggtg gatcctgcgt
     9901 gaggcgtgcc gccaggcgcg ggcctggcag gcggagtcct ccggtgtccc cccgttgatc
     9961 gtcagcgtca acctctcggc gggccagttc gagcgggacg ccctccccga ggaagttgcc
    10021 accatcgtgc gtgagaccgg gttcgagccg cgtcgcctcc agctcgagat cagcgaggcc
    10081 gtcctgatgc gcgacgaccc gcggatgttc gaccggctcg atgccctcaa agccataggc
    10141 gtgcgcctgg ccatcgacga cttcggcacc ggctacgcct ccctctccta tctcaagcgt
    10201 ctgccggtgg actgtctcaa gatcgatcgc tcgctcgtca agggcgtggg ctacgagacg
    10261 gaggacaccg cggtcatccg ggcggtggtc agcctcgccc acagcctcgg catcacggtc
    10321 accgcggaag gcatcgagac gcaggaccag ctcgtccagc tccgcgcggt cggctgcgac
    10381 cagggccagg gctacttctt cgcgcggccg gtctcggcgg accgggttcc ggagctcctc
    10441 gcgagcctcg gcgcggacga gcggaacggg agcccctcat ggctcggctg agtctccgcg
    10501 ggctcgcccg caccctcgtg gtgcggctga tcgggatcgc ggccttcacc ggcgccctcg
    10561 ggctcggcct gtcgtggctc ctcgagatgg cggtctccga gccggagtgg accgagaccg
    10621 ccgcggtcgc ccagcggtcg gtgaaccgca gcgggctgcc cgagttgctc gcgggccaga
    10681 acgcgcgcag ccccaagcgc tgggccgagg agagcgcgcg cttcgtccgg gagctgccgg
    10741 gcgtcgtcaa catcaaggtc tgggacccgc agggcacggt ggtgtgggcg gtgcagtccg
    10801 ggttcgtcgg ccagcgctcc gacggccacg agctgcgcga cgccatcagc gggcgcgtgg
    10861 cggtccgctt ctcctcggtg gccccgccga acgcagaggc ccagagcttc gtctcgccca
    10921 acgtggccga cctcttcgtc cccgtctacg gcccgggggc cgcgcgcgtg gtcggcgtgg
    10981 tcgagctctc ccaggcgccg ctgcgcatgg accaggcgca cgagcgctgg agcctgctcg
    11041 cctggaacat cgcgctcggc accggcggga tcctctgcct gatcctcctg cccgcggcgt
    11101 ggcgcagcca ccggcgcacg ttggcggaga ccagctcgac ctcgcagcgc tcggtacagc
    11161 gcacccagga gctgcagcgc accaccgagc agctccgcga cgccctcaag gcggtgaagc
    11221 agcgcgccga ggagaccgac cggatgctcg aggtgaccga ggccatcggc tcggcgatcg
    11281 agcagaaggc gctgttcgag gtcatcgcgc acggcgccgc gcgcgcgtgc cgagtcgatc
    11341 gcggctccat cttcctgcgc gacaagtcgg gccagctcat ggtcccggtc agctcgcagc
    11401 tcgccgaccg cgcgggcgcg gcccgaggcg cggacggggc cgagaccctg cgcgcgctcg
    11461 ccctcgagga gatgccctcg ttcctgctgg aggcggtgcg gcgccaggag ccggtggtca
    11521 tctccgaccc gggcagcgat ccgcgggtgc cgcagggctg ggtcggcctc ttccaggcca
    11581 agtccgcgct ggtggtgccg ctgatccacc agggccgcac cgcgggcgtg ctcatcctcg
    11641 accacctcaa gacgctgcac gtgttcaccg aggaggagat ccgcctcgcc accacgctgg
    11701 ccgcccaggc cgcggtggcc ctcgagaagg cgcggctcta cgacgagatc cagcagcgcc
    11761 tgcagcagac cgagacgctg ctcgcggtca gccaggccat cggctcggcc cctgatctcc
    11821 ccgaggcgat ccgccggacc acccgagaaa tggtgcggat cctgggcgcc gacaccggcg
    11881 tggcgtggtg catgacgggc ggcggcgacc gcttcatccc gctcgcgggc taccacgtgc
    11941 cgcgcgacct gcgcgagccg ctctcgggcc taccgctggc cccggacgac ccgctgatcg
    12001 acctcgtgaa gcgcctgcgc accgcgctgc actcgagcaa cagcaagacg gacccgcgct
    12061 tcaaccaggc cgcgctccag ctcctgcccc aggcgtcgct gctgatccag ccggtacagg
    12121 tcagcgacga gctgctcggc atcttcgcga tcgcctgggt ccgcgaggcc cacgtcttca
    12181 cgatggacga cctgcgcctg gccgacggca tcgccaagca ggccgcggtg gccatcgagc
    12241 tgcaccgcac ccagcgccac atcatcgagc aggagcggct caacgcggtc ggcaagatgg
    12301 cgagcggtct cgcccacgac ttcaacaacg cgctggtgcc gatctccggc tacgcggaga
    12361 tgctgctcga gcacccggag atcctccagg acgcggccaa gtccacccgc taccttaagc
    12421 tgatcatgac cggcgtggag gacgcggcga gtgtggtgag ccggctgcgc gagttctacc
    12481 gcaagcgtga ggagggcgag acctaccgac cgctctcgct caaccagatg gtggagcagg
    12541 cggtggccct cacccgcccc cgctggcgcg acgaggcgct cggcagcggc cgcacgatcc
    12601 tggtggaggc cgacatgcgg ccggtgcccc gcatcctggg cagcgagtcc gagatgcgcg
    12661 agctgctcgc caacctcatc ttcaacgcgg tggacgccat gcccgagggc ggcctgatcc
    12721 gcatccgcac cggcatgcgc gaagcctccg cgagcgagga gacgccgctg gctccccgcg
    12781 tgccccaggt cttcctggag gtgagcgaca ccggcaccgg catgaccgag gacgtgcgcc
    12841 gccgctgcct cgagcccttc ttctccacca agggcgagcg aggcaccggc ctcggcctgc
    12901 ccatggtgta cggcatcgtc aagcgccacc gcggcaccat ggacatcgag tccaccgtcg
    12961 gcaagggcac cacgttcatc gtcctgttgc ccgccgacac cagcctcgcc cgcgaggtcg
    13021 cgccgcgcgc gcaggccccc tcctccggat cgctccacgt cctggtggtg gacgacgagc
    13081 cgatcgcccg cgacgtgctc agcgagtacc tcatggggga cggccacacc gtggacaccg
    13141 cggtcaacgg gcgcgaagcc ttcgagaagt tcaaggcggg ccggtacgcg ctcgtcatca
    13201 cggaccgggc gatgccggaa gtgggcggcg atcagctcgc cgcgatggtc aaggaggtcg
    13261 cgcccgacac gccggtcatc ctgctcaccg gcttcggcga cctcatgaat gcggccggcg
    13321 agaagccgga cggcgtggac atggtggtga agaagccgat ccggctcgcc accctccgcg
    13381 aggtcctctc caagatgacc gaccccacgc gcgccgccga gaccgcgccc agcccgaccg
    13441 cctgacgcgc ctcaacgacc gaagacgacc gcgccccccg ccgcgctgat cagcaccacc
    13501 agccacggcg ccgccttcca gaccacgagc agggcgaacg ccaccagggc cagcgcgaag
    13561 tcggcggggc ctccgatgcc cttggtccac accgggtcgt agagcgcgcc caggagcacg
    13621 ccgaccaccg cggcgttgac gccccgcagg ctcgcgggca tgagcgccac gcgcccgagc
    13681 gtcaccaaga acggcagcac gccgagcacg aggagcaggc cgggcaggaa gatcgcgacc
    13741 aggcacagcg ccgcgcccgg ccacccggcg ggcgggaccg tcatgatcgc ccccaggtag
    13801 gcggcgaagg tgaagagcgg cccgggcacc gcctgcgcgg ccccgtagcc ggagagaaac
    13861 gcgtcctcgc tcacccagcc gggctccacc accgacgcat gcagcagcgg cagcacgacg
    13921 tgcccaccgc cgaagacgaa tgcgccggcc cgatagaacg cctcggcgac cgcgagcgcc
    13981 ggcacgggcg tgacggcggc ggcgatgggc agcccgatca gcagcagggc gaacagcgcg
    14041 agcgcggcga ccgcgagggc gcgcggtatc ggcaccgcca accgctcgga ggcgacgatc
    14101 gcccccggct ggcggcagag ccatgcgccg gcggcggcgc ccgcgaggat cgcggcgatc
    14161 tgcccgatcg cggtgggggc ggcggtcacg acgagggtgg cgacgaccgc gatggtggcc
    14221 cgggggcgat ccggacagag gctccgcgcc atgctccaca ccgcctgcgc gaccaccgcc
    14281 accgccacca cctgcagccc gtggagcatc ccggtgctga gcgggccggt caacgcggcg
    14341 gctccctgcg cgaagaggac catggccacc gcggacggga gcgtgaagcc gagccaggcg
    14401 accagcccgc cggcgagccc gccgcgcgag aggcccaccg cgaagctcac ctggctgctg
    14461 gccggcccgg gcaggagctg gcagagcgct accaggtccg cgtaggccgc ctcgctcagc
    14521 cagcgccgcc gctcgacgaa ctccgtccgg tagtaaccca ggtgcgcgat cgggccgccg
    14581 aaggacgtga gccccaagcg caggaagacg gcgaagatgg gccccatcgg ccggctagcc
    14641 cggatcgacc atcgtctccg ggcgcaccgc gcgatcgaac tcctcggcgc tcagatggcc
    14701 gagcgccagc gtcgcctccc tgagcgtgag cccctccgcg tgcgccttct tggcgatcgc
    14761 ggcggcccgg tcgtagccga tgatcggggc cagcgcggtg accagcatga gcgagcgctc
    14821 gacgagggcc cggatgcgct cgcggtccgg ctcgatgccc cgggcgcagt accggtcgaa
    14881 cgagtcgcac gcgtccgcga gcagccgcac cgagcccagc acgttgtgga tgatgagcgg
    14941 cttgaagacg ttcagctcga agttgccgga ggcgccgccg atccccaccg ccacgtcgtt
    15001 gcccatcacc tgggcgcaca ccatggtgag cgcctcgcac tgggtcggat tgaccttgcc
    15061 cggcatgatc gagctgcccg gctcgttctc cgggatgcgc agctcgccga tgccgctgcg
    15121 cggccccgac gcgagccagc gcacgtcgtt ggcgatcttg gtgagcgccc cggccagcgt
    15181 ccgcagcgtc ccgtgggcgt tgaccagggc atcgtgcgcg gccagggcct cgaacttgtt
    15241 gggcgcggag acgaaggccc gcccggtcag atccgcgatg acgcgggccg cccgctcccc
    15301 gaagccccgc ggggcgttga ggccggtgcc gaccgcggtg ccgcccagcg cgagctcccg
    15361 cagatgcggc agcgccgcgc gcacgtgctc gagaccgtga tcgagctggc tcgcccagcc
    15421 cgagatctcc tgcccgagcg tgagcggcac cgcgtcctgc aggtgcgtgc gcccgatctt
    15481 gacgatgtcg tcgagcgcgc gcgccttcgc ctgcagcgtg tcgcgcagcc cgcccaccgc
    15541 ggggatcagc cgtccctcga tctgctcgac ggcggcgatg tgcatcgcgg tgggaaacgt
    15601 gtcgttggag gactggctgc ggttgacgtc gtcgttggga tgcaccggct gctgggcgcc
    15661 gaggcggccg ccgagcagct cgttggcgcg gttggcaatc acctcgttgg cgttcatgtt
    15721 agtctgcgtg ccgctgcccg tctgccagac taccaaggga aagtgggcgt ccaggttgcc
    15781 ctcgatgacc tcctcggccg cctgcacgat ggcccgcgcc ttgtcctcgg gcagcaggcc
    15841 gagctcccgg ttcacgaacg cggacgcctt cttgaggatg cccagggccc ggatcatctc
    15901 gcgcgggaag tgatcgcggc cgatccggaa gtgctcgagc gaccgctggg tctgcgcgcc
    15961 ccagtagcga tcgcccggca cctcgatggg gcccatcgtg tccttctcgg ttcgcgtcgt
    16021 ggtcatgggg cctcctcgtg gctggcttga gccgcctgtc gccttcgcat agtatcgccg
    16081 ggaggggaga gcacgccatg gactgttgcg gtcacgaggg cccgggcggc gacgcccgct
    16141 tcgacttccg ccgggtggcc gacggcgtcc acgtcgcggt cgccgcgccc gcgtacaagg
    16201 tcaactccaa caccgcgatc atcgagagcg acgacggcgc ggtgatcgtg gacacgcact
    16261 ccaagccgtc cgcggcgcgg gtgatcgtgg accgcctccg cgacatcacg gcccggccgg
    16321 tgcgctacgt cgtcaacacc cacttccact gggaccactg gcacggcaac gaggtctacc
    16381 ccgcggcgta tccgggcgcg gagatcatca ccaaccagct cacccgcgag gcgatggtca
    16441 agaagggctt caagcgcata caggaccacg tgcggcaggt acccgcggag atcgcccgtc
    16501 tccgcgccga gctggccgcc gcgaccggcc ccgagccgcg cgcgaagctc caggccgatc
    16561 tcggcctcgc cgagtcgtat ctcgccgagg tccgggcgat gaagccggcg ctgcccacca
    16621 tggccttcga gcggacgatg acgctctacc ggcgcgaccg ggagatccag ctcctgtatc
    16681 tgggccgggc ccacaccgag ggcgacgtct tcgtctacct gccgaaggag aaggtggtca
    16741 tcaccggcga cgcggtcatc gggtggacgc cgttcatggg cgacggctat ccggaggact
    16801 gggtcacgac cctggatcgg ctcgccgagc tcccctggga caccctcgtc atgggccacg
    16861 gcgagccggc ggggcgggac tggctgcgga ccttccgcgg atacatccac gacatggtgg
    16921 aggccgtgcg cgaggaagcc gccaccggcg cgagcctgga cgaggtcaag cagcgcgtga
    16981 ccgcgcacct ggccccgacc tacgagcccg cgttcgccgc ctatcagcac tatcgcccgt
    17041 ggcgccaggg cctgctggcc aacatcgagc gcacctaccc gctggtgagc tgagcgcgcg
    17101 atggcgacgg acgaggcgat cttcaacccg ctcacccccg agttccacgc caacccgtac
    17161 ccgttctacc agcgcctgcg cgagaccgat ccggtccacc tgagtccgct cggcctctgg
    17221 gtcctcacgc gctacgacga ctgcgtgacg tccctgcgcg atccccgctt cggccgcgac
    17281 ggcttcgagg cgatcctgtc cgcccagtac ggcgaggaat cggagaccgg ccggctgccc
    17341 cgctccatgc tcttccggga tccgccggac cacacgcgcc tccgctcgct cgtcaatcgc
    17401 gcgttcaccc cgcgggtgat cgagggcatg cgcgggcaga tccaggccgt ggtggaccgg
    17461 ctgctcgacc aggtggaggg gcgcggccgc atggacgtca tcgccgacct ggcctatccg
    17521 ctgccggtca ccgtgatctg cgacatgctc ggggtgccgg tgggcgatca cgagcagatg
    17581 cgggactggt cgtccgacat catccgcagt ctcgacgcca tcgggatccc gtcggacgag
    17641 tcggtggtcg agcgcgggcg cgtcgggcgg cggggcatcg ccgagtactt tcgcgcgctg
    17701 ctgcccgagc ggcgccggca cccgcgcgcc gatctcctct cctcgctgat cgcggtggaa
    17761 gagcagggcg accggctcac cgagggcgag ctgctcgcga cctgcgtgct gctcttcatc
    17821 gcggggcacg agaccaccgt caacctgatc ggcaacggcc tcctcgccct gctgcggcac
    17881 cccgcggagc tcgagcgcct gcgcgtggag cccgggctga tcggctccgc ggtggaggag
    17941 ctgctgcgct tcgacagtcc cgtgcagcgc acggcccgca tcacgaacgc ggaggtggag
    18001 gtgggcggca agacgctgcc gccgggcgcc ttcgtggtca ccgccatcgg cgcggccaac
    18061 cgggatccgc gctacttccc cgatcccgac cgcctggaca tcggccggag cgagaaccgc
    18121 cacatcgcct tcgggttcgg gatccacttc tgcctgggcg ctccgctggc ccgcgtggag
    18181 ggccagctcg ccctgggcac cttgatgcgg cggatgccaa agctccggct ggcgagtccg
    18241 gatctcgaat ggcgggagtc ctcgacgcta cgcggcctca aggccctccc cgtcgagctc
    18301 tgatctcacg ccaccgtttc cactagaccc cggagccgtc ccggtgggtc cccggcggga
    18361 caattggccg cgccacgaac cggcgcatgt cgcccttcgc ccgccggccc tcctcctcga
    18421 tctggaagcc gccggcctcg accgcgccct cggtatcgcg gttcggatga cagccgccgg
    18481 tgatctgcgt ccaggccggc tgcaggagat cctggagccg ggccttccac gcccgcgtcg
    18541 cgcgcacgtg ctcgagcatc cggagctggc cgcccggccg cagcacgcgc cggacttcca
    18601 gcaggccctg gtgcggatcc cggacgctgc aaaagacgag gccgctcacg accgtgtcga
    18661 aggccgcgtc gcggaacggg agggcctcgg cgctgccgac caccagcggc acccgcgggg
    18721 cgcgacgacg ggcgcgctgc agcacggccc atgacggctc gaggcccacg gtgcgcgtcc
    18781 ccgccggcag gagcggcagg ttgcgcccgg tgccgcagcc caggtcgagc acgcggccgc
    18841 gcgcgccgct cacgagccag ctccgccagc gggccaggcc cgtagcttcg cacagggcgc
    18901 agaccgcgtc gtagagccac ggaatctgct cgaccccccg catcgggacc cttaggtcag
    18961 caggtccttc ttgaagagct cgagagcctg cacgtgctcg gccggcgtct tcaagcccag
    19021 tcccaccgtg tggacgcaaa ggtgcgtgat tccgcgcatc gcgcgccagg ccgtaagctc
    19081 ggccgcgcgc cgctccggtg ggacctgggc gagggtaatc cgcccctcga tgccgaagtc
    19141 ggccggatcg cgcccggcct cccggatcaa gccgtgcagc cggtccacga tggcctgggc
    19201 ctcgggcccg ggccggaaat ggggcatcca cccgtcggcc agccgtgccg cgcggcggac
    19261 gacgggctcg ctctccccgc ccatccagat cgggatcgtc ggccgcgggg gcaagggatt
    19321 gaggcccgcg tcggtgaccc ggtgccactg gcccgcgatc gtcaccaggt cgcgggccca
    19381 gagcgcgcgc agcaccgcga tctgctcctc gatgcgcgcg ccgcggttcg tgaagcgctc
    19441 gccgagggcc tcgaattcca ccgggttcca gccgaccccg acgcccagcc gcagccgccc
    19501 gctcgacagc acgtccaccg cggcggcctg cttcgctacg agcgtggtct ggcgctgggg
    19561 caggatcagg atgccggtgg ccagctccac gcggcgggtg gccgcggcga ggaacccgaa
    19621 caggacgaag ggctcgtgga acgagtgacg atacgtgtag gggcccttcc agccgccggg
    19681 ccggtcggga ttcgcgccga gcacgtggtc gaaggtgaga atgtgcgtgt agcccatcgc
    19741 ctcgaccgcc tgggcgtagt cacgaatcgc tcccggatcc gccccgatct cgacctgcgg
    19801 gaagaccacg ccgatgtgca cggcgggagt ctagcacgat ggcggccggt cctccggggc
    19861 acccccgaag aggggcagcg ccccgtgtcc ccccgcccgc tcgctcgaga tcagccggag
    19921 cttggttctg acgccgccgc gcgcggagaa gccgccgacc ttgccgttcg cggcgagcac
    19981 gcgatgacac ggcacgatga tcgcgaacgg gttctgaccc agcgcctgcc ccacgtcgcg
    20041 cgcggcgccc ggcgcgccca tgcgcgccgc gacctcaccg taggacagcg tcgtgccggg
    20101 cgggatcgtg cgcgcgactt cgtacacgcg ccgatgaaac ggcggcaccc cgtccatgtc
    20161 gagggtcacg gtgtcgagct cggagggctc cccccggagc agcgccacca cgcccgcgat
    20221 ggcccgccgc accggcgcgg gcggcggtgc ctcgcgcgtg tccggaaagc gccgccgcag
    20281 ccgcgctcgc gtctcggcct cgcccccttc cgggagctgc acgcccacga ccccgcggcc
    20341 gttccacgcg atcccgcacg gcccgatcgc ggtgtcgaag agcgcgaagc cggtctctag
    20401 ccctgtcgcc attgacggat ctgctccgcg tgctcgcgat agtgccccgc gccggccccg
    20461 tcgaatggcc cgcgggcggc gccgcccgcg gcgaagagcg cctccggcag cgccgcggcc
    20521 acggccagca gcttgcggtg ggaggcctcc aggtccgcca ccacgtcggc ggtcttcaca
    20581 cccgccttct ggtccacgaa gcgcgcgttc cacgcatcga agtcatcgta cgtccccttc
    20641 gggtacggcg catccccctt cgcgacccgg cccagggccg tcgccatctc gtcgtgccag
    20701 cccgagatgt gaatcaggat ctcgcgcacg ccccaggtcc cgagccagac gcggcgcatg
    20761 tcctcgtcgg tcagcccgtc gatcgctccc cgcagctccc cgaatgccag atccgcgtcc
    20821 cgcagcagcg tggccttgtc gctcatcggt ccctccccgg tggtggtggc tcgcttccgg
    20881 tgcgtgagac cccggccggg ccccgttgtg aaaggtgccc ctgcgaaaag tgatagattc
    20941 gtccgggacc gcgaccggca cgaggagacc caatggccat tcggatcgag cgaagcgacg
    21001 tggtggcgac gatcgtcatg gaccgccccg aggcgcggaa cgcggtggac ggtcccgcgg
    21061 cgcgggcgct gatcgaggcc ttcgaggagt tcgagcggga cgaggccctc cgggtcgccg
    21121 tgctctgggg cgcgggcggc accttctgcg cgggggcgga cctcaaggcg cgggggaccg
    21181 cgaacgggtt gaccctgtcg cccgagggcc ccggccccat gggccccacc cggatggcgt
    21241 tcaccaagcc gacgatcgcc gccgtcgagg gccacgcggt ggcgggcggg ctcgagctct
    21301 cgctgatgtg cgacctgcgc gtggccgggg acaccgcggt cttcgga
//
LOCUS       DASUFA010000159         7285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_367597, whole genome shotgun sequence.
ACCESSION   DASUFA010000159 DASUFA010000000
VERSION     DASUFA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367597"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFX87_05775"
     CDS             complement(<1..1175)
                     /locus_tag="VFX87_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX4994455.1"
                     /translation="MRAPPVWAASIGQALATSAATERGLSSPEAAARLRASGPNDLAP
                     PRRFEAIRELLRYFANPLVLILLVASAVSTAFGQPVSAVIIALMLALSVALNFTQAYR
                     SQRAAARLRRQVGQTATVIRDGVEREVPVREVVVGDVIHLKAGDLVPADCRLLSTRDL
                     FVNEAALTGESLPREKQAMPGALAAGGLGEAVTAVFRGTSVISGLGVAVVVATGPATE
                     FGRVATSLAARPPETEFERGTRRFGFLILQVVIFLVLFVFLVNALLKRDLLETFLFSV
                     ALAVGLTPELLPMIVSVTLASGAVRMARKKVIVKQLAAIENFGSMDILCSDKTGTLTH
                     GEITVDRHLNIEGENDEAVIRLAALNSVYQTGLRSPMDDAILKHEHPAVARHERVDE"
     gene            complement(1176..2915)
                     /locus_tag="VFX87_05780"
     CDS             complement(1176..2915)
                     /locus_tag="VFX87_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX4994456.1"
                     /translation="MFFKALACDYDGTLAFEDRIPPVVHEALERARTAGLRLILVTGR
                     TFFELTRVCDCLELFDAVVAENGAVLYYPGSAMIRDQGPPVPDRLLAELDRRGIYYQV
                     GRVIVGTGRGDEGALKEALAAAGVTRDLIYNRAALMLLPAGVSKGSGVLQALRVLSLS
                     PHDVLALGDAENDLPLFDACGFSGCPEDSLPAVRERVDWVFPGVHGAGIAAAITDQIL
                     PDRLTVRHSPRHRISIGWVATTSGPVTIPARGVNVLIHGDPHSGKSWLAGALVERLVA
                     ARYAVCVIDPEGDYQVLAHLPGVTRTEVQEPDDVDRALDGHLQDPSASTVLDLSTLPH
                     AVKVVVIERALRKIREIRRSIGRPHWILIDEAHYSLHGGGVTADALDVAARGFCFVTY
                     RPSWVREEVIRAVDIFVLARTTRSEELAFLGSTLPAVVSEGAGISEALARLPRGEFLL
                     VQRNRERQPSALTFVAAPRQTVHVRHLTKYVDSCVPPGREFSFRGPDGRVRASADSLH
                     SFRRVVETAPDDVLAHHAEHGDFSRWVRDVFADTELARQLRKIEARWRRRELAELRRT
                     IDAVITVRYGADD"
     gene            3118..3657
                     /gene="pal"
                     /locus_tag="VFX87_05785"
     CDS             3118..3657
                     /gene="pal"
                     /locus_tag="VFX87_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX4994457.1"
                     /translation="MKTIRPLLPLMVLISLLVGCQTAKAPSALAGATPDLERSTAVVA
                     IEPPDADTLSAPGLPPPPEQFVPVPRLADIHFDYDAYTIRPEDTPILDENAAWIRANP
                     GGLVLIEGHTDERGSDDYNLGLGDLRAAAARNYLIAHGIPAERMVAISYGKERPACIE
                     HNEACWARNRRARFLVKPL"
     gene            3707..4813
                     /locus_tag="VFX87_05790"
     CDS             3707..4813
                     /locus_tag="VFX87_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX4994458.1"
                     /translation="MPFVARGIGDELGPDKVVLLREPRVGLEAVVVIDNVACGPAIGG
                     VRMAPDVTVEEVSRLARAMTLKNAAAGLAHGGGKSGIAADPAAPPADKERLIRAFARA
                     IRSLTEYIPGPDMGTDERCMAWIKDEVDRAVGLPRVLGGIPLDEIGATGWGVAIAAEI
                     AAPEAGIALAGARVAIEGFGAVGQHAARFLAARGCRMVAASDSRGAIHDPAGLPIDAL
                     IAHKRAGRPLTDFPGGRMIGRDALIGVPCEIWIPAARPDTITADNVSGLQAKLVIQGA
                     NIPATEPAERWMHAHRILSVPDFIANAGGVICAAIEYHGGSQVQAFATIEERIRANTR
                     ETLDGVRTDGIVPREAATRMALARVREAMRYRRP"
     gene            4827..6167
                     /locus_tag="VFX87_05795"
     CDS             4827..6167
                     /locus_tag="VFX87_05795"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX4994459.1"
                     /translation="MSRRGGGTGRRAGARRVLPALLAIALTVPLPLLAQPPVAAGSLS
                     PEELEQIAAPIALYPDALLAQALMAATYPLEVVMAARFARTNPGLKGEALDEALTAQN
                     WDDSVKSLVVFPQVLSMMDEQLAWTQKLGDAFLAQRAELMDAIQRLRARAQAEGTLAS
                     TPQQVVTVEPTPGQPIIGIEPAASGVVYVPTYDPLVVYGPWPYPAYPPYYYYPPGWVV
                     GAFFTFGVGIVVGTALWGRCDWHHHRIDLDVDRYRRFTRVVNTDRNRSELIRGRLIPG
                     GERLTWEHSPEHRRGGLYRTEGLQHLFGRPVAPDPTAREMFRGREDLGRPEPGRAPGA
                     LIRPGPQPGRAPGADVRPGPAPGPRLERGPSAPAPGGPTVRGVPPREPGAFQGLGRGQ
                     DTRSYSDRGRESRRNIAPPTRPVPRPSPSRPAPSRPAPSGPSHGGAAPGGGARR"
     gene            6164..7072
                     /locus_tag="VFX87_05800"
     CDS             6164..7072
                     /locus_tag="VFX87_05800"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HEX4994460.1"
                     /translation="MMSRIALAVVLLWASGSSAATATHMFFPSPDAAVMALVQAARAS
                     DTATLLRVLGPDARALIASGDEVADRQSRERFVRAYDEAHRLSEVDAGTRVLTVGKDE
                     WPLPIPLVRDAAGWWFDTARGREEILNRRIGRNELNAAQVCLAYVDAQREYYVRDPDG
                     DALLQYAQRFRSTSGKRDGLYWDAQPGEPPSPLGALVARAEAEGYLAKRPSGARIPYW
                     GYYYRIVKAQGPHAPGGAYDYVVRGSMIGGFALVAYPAEYGASGVMTFIVNHDGIVYQ
                     KDLGPSTAATARALTHFDPDPTWSRF"
BASE COUNT         1070 a         2659 c         2581 g          975 t
ORIGIN      
        1 atctcgtcca cgcgctcgtg ccgggcgacc gccgggtgct cgtgcttcag gatcgcgtcg
       61 tccatggggc tgcgcaggcc ggtctggtac acgctgttca gcgcggccag ccggatcacc
      121 gcctcgtcgt tctccccctc gatgttcagg tgacggtcca ccgtgatctc gccgtgggtg
      181 agcgtgcccg tcttgtcgct gcacaggatg tccatgctcc cgaagttctc gatggcggcg
      241 agctgcttga cgatgacctt tttccgcgcc atgcgtaccg ccccgctcgc cagcgtgacc
      301 gacacgatca tggggagcag ctcgggcgtg agccccaccg ccagcgccac cgagaacagg
      361 aacgtctcca gcagatcgcg cttcaacagg gcgttgacca ggaagacgaa gagcacgagg
      421 aagatgacga cctgcaggat caggaaaccg aaccggcgcg tgccccgctc gaactcggtc
      481 tccggggggc gcgcggcaag gctcgtggcc acgcgcccga actcggtggc cggaccggtg
      541 gccaccacca ccgccactcc cagaccgctg atcaccgagg tgccccgaaa gacggccgtc
      601 accgcctccc cgagcccgcc ggccgccaga gcgccgggca tcgcctgctt ctcgcggggc
      661 agcgactcgc cggtgagcgc ggcctcgttg acgaacaggt cccttgtcga gaggagccgg
      721 cagtccgcgg gcaccagatc cccggccttg aggtggatca cgtctcccac caccacctcc
      781 cgcaccggca cctcgcgctc gaccccgtcc cggatcaccg tcgcggtctg gcccacctgc
      841 cggcggaggc gcgcggccgc ccgctgggag cggtacgcct gggtgaagtt cagggccacg
      901 ctcagcgcga gcatcagcgc gatgatcacc gcgctgaccg gctggccgaa cgcggtcgag
      961 accgcgctgg ccacgagcag gatcagcacg agcgggttgg cgaagtagcg aagcagctcg
     1021 cggatggcct cgaagcggcg gggcggcgcc agatcgttcg gtcccgacgc ccgcaggcgc
     1081 gcggcggcct ccggcgatga cagcccgcgc tccgtggcgg cggacgtggc cagggcttgc
     1141 ccgatgctcg ccgcccagac cggcggagcc cgcatctagt catccgctcc gtagcggacc
     1201 gtgatgaccg cgtcgatggt ccgccgcagc tccgccagct cccggcggcg ccagcgcgcc
     1261 tcgatcttcc ggagctgccg cgccagctcg gtgtcggcga acacgtcgcg gacccagcgt
     1321 gagaagtcgc cgtgctcggc gtggtgtgcg agcacgtcgt cgggtgcggt ctcgaccacg
     1381 cggcgaaacg agtgcaggct gtccgccgac gcgcgcaccc gcccgtccgg gccgcgaaag
     1441 ctgaactccc ggccgggcgg cacgcacgag tccacgtact tcgtcagatg ccggacgtgg
     1501 acggtctgcc gcggcgccgc cacgaaggtc agcgcgctgg gttggcgctc gcgattgcgc
     1561 tggacgagca ggaactcccc gcggggcagg cgggcgaggg cctccgagat gcccgcccct
     1621 tcgctcacca ccgccggcaa cgtcgagccg agaaacgcca gttcctcgct cctggtggtg
     1681 cgggccagga cgaagatatc cacggcccgg atgacttcct cccgcaccca gctcgggcgg
     1741 tacgtcacga aacagaaccc gcgggccgcg acgtccagcg cgtcggcggt gacccctccg
     1801 ccgtgcaggg aatagtgcgc ctcgtcgatg aggatccagt gcgggcggcc gatgctccgc
     1861 ctgatctccc ggatcttccg cagggcccgc tcgatgacga cgaccttgac cgcgtgcggc
     1921 agggtggaca gatccaggac cgtggacgcg gaggggtctt gcaggtgccc gtcgagtgcg
     1981 cggtccacgt cgtcaggctc ctggacctcc gtccgcgtca cgccgggcag atgcgccagc
     2041 acctggtagt ccccttcggg atcgatgacg cagaccgcgt agcgcgcggc caccaggcgc
     2101 tccacgagcg cgcccgccag ccacgactta cccgaatgcg gatcgccgtg gatcagtacg
     2161 ttgacgccgc gggccgggat cgtgaccggc cccgacgttg tcgccaccca tccgatggag
     2221 attcgatgcc ggggagagtg ccggacggtg aggcggtccg gcaggatctg gtcggtgatg
     2281 gcggcggcga tcccggcgcc gtggactccg gggaagaccc agtccactcg ctcccggacg
     2341 gccggaaggc tgtcctccgg gcagccgctg aacccgcacg cgtcgaagag cggcagatcg
     2401 ttctccgcgt cgcccagggc cagcacgtcg tgcggcgaca ggctcaggac gcgcagcgct
     2461 tgcagcaccc ctgagccctt ggacacgccg gcgggcagca gcatgagcgc ggcgcggttg
     2521 tagatgagat cccgcgtcac gcccgcggcg gccagcgcct ccttcagggc accttcgtcc
     2581 ccgcggcccg tgcccacgat gacccggccc acctggtagt agatcccgcg ccggtcgagc
     2641 tcggcgagga gccggtccgg caccgggggg ccctggtcgc ggatcatggc cgagccgggg
     2701 tagtagagca cggcgccgtt ctcggccacg acggcatcga acagctccag gcagtcgcag
     2761 acccgcgtca gctcgaagaa ggttcgcccg gtgacgagga tgaggcggag gcccgccgtg
     2821 cgggcccgct ccagcgcctc gtgaaccacg ggagggatcc ggtcctcgaa cgcgagggtg
     2881 ccatcgtagt cgcaggcgag cgccttgaag aacacgtcag gtcaatggga gcaagccgag
     2941 tgccaccact caaacaacac aagatggtga gaatcgccga cccggccggc gggacgcggg
     3001 acaccggcgc cccactgtcg tggacattcc gtccgcgcgg gagccccggg ccggggcgca
     3061 agtagcgtcc tgatacccct ttccagaccg gcacctccct tgcaccctcc gatcgccatg
     3121 aagacgatcc gtccgctgct gccgctgatg gttctcatct ccttgctggt cgggtgccag
     3181 acggcgaagg cgccgtccgc cctcgccggc gccacgcctg acctcgagcg atccacggcg
     3241 gtggtcgcca tcgagccgcc cgatgccgat acgctatccg cgccgggcct gccgccaccg
     3301 cccgagcagt tcgtgccggt cccgcgcctg gccgatatcc acttcgacta cgatgcctac
     3361 acgatccgcc cagaggatac gccgatcctc gacgagaacg cggcgtggat ccgggcgaat
     3421 cccggcggcc tggtcctgat cgaggggcac accgacgagc gagggagcga cgactacaac
     3481 ctcggcctgg gcgatctgcg agcggcggcc gcgaggaact acctgatcgc gcacggtatc
     3541 ccggcggagc ggatggtggc gatctcgtat ggcaaggagc ggccggcctg catcgagcac
     3601 aacgaggcgt gctgggcccg gaaccgccgg gcccgattcc tcgtcaagcc gctctgaggg
     3661 tgacatgagc cctcgcgatc cccagggcgt cacgggccac gccggcatgc ccttcgtggc
     3721 gcgcggaatc ggagatgagc tggggcccga caaggtcgtg ctgctgcgcg agccccgtgt
     3781 cgggctcgag gccgtggtcg tcatcgacaa cgtggcgtgt gggccggcca tcggcggggt
     3841 gcgcatggcc ccggacgtca ccgtcgagga ggtctcgcgg ctcgcgcggg ctatgacctt
     3901 gaagaacgcg gccgccggcc tggcccacgg cggaggcaag tccggcatcg ccgccgatcc
     3961 cgccgcgccc ccggcggaca aggagcgcct gatccgcgcg ttcgcacggg cgatccgctc
     4021 gctcaccgaa tacatacccg gacccgacat gggcaccgac gagcgctgca tggcctggat
     4081 caaggacgag gtcgaccgcg ccgtcggttt gccgcgcgtg ctgggcggca tcccgctcga
     4141 cgagatcggg gccaccggct ggggggtcgc gatcgcggcg gagatcgccg cgccggaggc
     4201 ggggatcgcg ctggccgggg cgcgcgtcgc gatcgagggc ttcggggccg tggggcagca
     4261 cgcggcccgg ttcctggccg cgcgcgggtg ccggatggtc gccgcctccg acagccgggg
     4321 ggccatccac gaccccgcgg ggctccccat cgacgcgctg atcgcccaca agcgggccgg
     4381 gcggcccctg accgacttcc ccggcggccg gatgatcggc cgggacgcgc tgatcggcgt
     4441 tccgtgcgag atctggattc ccgccgcgcg accggacacg atcaccgcgg acaacgtgag
     4501 cggcctccag gccaagctgg tcatccaggg cgcgaacatc cccgccaccg agcccgccga
     4561 gcgatggatg cacgcccacc gcatcctgag cgtgcccgac ttcatcgcca acgcgggcgg
     4621 cgtcatctgc gccgcgatcg aataccacgg cggctcccag gtgcaggcct tcgcgaccat
     4681 cgaggagcgc atccgcgcca acacccgcga gaccctcgac ggggttcgca cggacgggat
     4741 cgtcccacga gaggcggcca cccggatggc gctcgcccgc gtgcgcgaag cgatgcgtta
     4801 ccgcaggccg tgacgcggag gcgatggtga gccgtcgagg cggcgggaca gggcggcggg
     4861 ccggcgcgcg ccgtgtcctc cccgccctgc tggccatcgc tctcacggtt cccctgcctc
     4921 tgctcgccca gccgccggtc gcggccgggt cgctcagccc cgaggagctg gagcagatcg
     4981 ccgcaccgat cgcactctat ccggatgcgt tgctggcgca ggcgctgatg gcggccacct
     5041 acccgctcga ggtagtgatg gcggctcgct tcgcccggac caaccccggt ctgaagggag
     5101 aggcgctgga cgaagcgctc accgcgcaga actgggacga cagcgtgaaa tccctcgtcg
     5161 tctttcccca ggtcctgagc atgatggacg agcagctcgc gtggacccag aagctggggg
     5221 acgcgttcct ggcgcaacgg gcggagctga tggacgcgat ccagcgcctg cgcgctcgag
     5281 cccaggccga gggcacgctg gccagcacac cgcagcaggt ggtcaccgtg gagcccacgc
     5341 cggggcagcc gatcatcggc atcgagccgg ccgcttccgg tgtggtctat gtaccgacct
     5401 acgacccgct ggttgtctac ggcccctggc cctacccggc ctatccgccc tactactatt
     5461 acccgcccgg atgggtcgtg ggggccttct tcaccttcgg cgtcggcatc gtcgtgggaa
     5521 cggcgctgtg gggccggtgt gactggcacc accaccggat cgatctcgat gtcgaccgat
     5581 accggcgctt cacgcgcgtc gtgaacaccg accggaatcg cagcgagctg atccgcggga
     5641 gactcatccc gggaggcgag cgcttgacct gggagcatag ccccgagcat cggcggggcg
     5701 gcctgtaccg caccgaaggc ctgcagcacc tgttcggtcg gccggtggcc ccggatccca
     5761 cggcccggga gatgttccgc ggccgggagg acctcggacg gcctgagccc ggacgcgctc
     5821 cgggagccct gatccggccg gggcctcagc ccggacgcgc tccgggagcg gacgtccgcc
     5881 ccggcccggc accggggccc aggctggagc gaggtccgag cgctcccgcg ccgggtggcc
     5941 cgaccgtccg cggcgtgccg ccccgggagc cgggcgcctt ccaggggctc gggcggggcc
     6001 aggacacgcg aagctacagc gatcgcgggc gcgagagccg tcggaacatc gccccgccga
     6061 ccagacctgt cccgcgacct tccccgtctc gaccggctcc gtctcgtccc gccccctcgg
     6121 gccccagcca cggcggggcg gcgccgggcg gcggagctcg ccgatgatgt cgcggatcgc
     6181 cctggccgtg gtcctcctgt gggcgtcggg ctcgagcgcc gcgacggcca cgcacatgtt
     6241 cttcccgtct ccggacgccg ccgtgatggc cctcgtgcag gcggcgcggg cgagcgacac
     6301 cgcgacattg ctgcgcgtcc ttgggccgga cgcccgggcg ctgatcgcct ccggtgacga
     6361 ggtggccgac cgacagagcc gggagcgctt cgtccgcgcc tacgacgaag cccaccggct
     6421 ctccgaggtc gacgcgggga cgcgcgttct cacggtcggc aaggacgagt ggcccctgcc
     6481 gatcccgctc gtccgggacg cggcgggctg gtggttcgac accgcccggg gccgggagga
     6541 gatcctcaac cggcgcatcg ggcggaacga gctcaacgcg gcccaggtct gcctggccta
     6601 cgtcgacgcg cagcgcgagt actacgtgcg cgatcccgac ggggacgcgc tgctccagta
     6661 cgcgcagcga ttccgcagca cctccggcaa gcgggacgga ctgtactggg acgcgcagcc
     6721 cggcgagccg ccgagcccgc tgggcgcgct ggtcgcccgg gccgaggccg aaggctatct
     6781 ggcgaagcgc ccgagcggcg cgcgcatccc ctactggggg tactattacc gcatcgtgaa
     6841 ggctcaggga ccgcacgcgc cgggcggagc gtacgactac gtcgtccggg gcagcatgat
     6901 cggcggcttc gcgctggtcg cgtacccggc cgagtacggc gcctcgggcg tgatgacctt
     6961 catcgtcaac cacgacggga tcgtctacca gaaggacctg ggcccgagca ccgcggcgac
     7021 cgcgcgggcg ctcacccact tcgaccccga ccccacctgg tcgcgcttct gagccacccg
     7081 ccccccgcgg ccgagccgga tgcgctcccc gtccgcgggt tccggggcag cgccgaggag
     7141 atcgagcggc agtggtacga gcaggtctac cgcggccgcg gcgaccggat ggcccagctc
     7201 acgtggcgcg cggtgctgat gggctcgctg ctgggcggga tgctctccct caccaacctc
     7261 tacatcgggc tcaaggcggg ttggg
//
LOCUS       DASUFA010000160         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1624016, whole genome shotgun sequence.
ACCESSION   DASUFA010000160 DASUFA010000000
VERSION     DASUFA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624016"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFX87_05805"
     CDS             complement(<1..297)
                     /locus_tag="VFX87_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX4994461.1"
                     /translation="MKDGRAALTPEEYARVEAQAAAEYPGECCGVVLARGDRPADRLL
                     LPCRNIQNDLHQKDPARHPRDARTAYYIDPRDLLTIGRREAEGFRVAIIYHSHID"
     gene            complement(294..638)
                     /locus_tag="VFX87_05810"
     CDS             complement(294..638)
                     /locus_tag="VFX87_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX4994462.1"
                     /translation="MSVQVYIPTPFRRATSNRDRVSLEAPDVKGLLDGLEAQFAGLKG
                     LVRNEQGQIHHHVNIYVNSEAIEALKGLDTALKDGDEVSIIPALAGGQAALAGGQAVR
                     AGGACAEAHLEA"
     gene            complement(651..1595)
                     /gene="cysK"
                     /locus_tag="VFX87_05815"
     CDS             complement(651..1595)
                     /gene="cysK"
                     /locus_tag="VFX87_05815"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX4994463.1"
                     /translation="MTQKKTTATWRPRLAASVLDLVGGTPLVRLNQLPRPGGATVLAK
                     MESLNPGGSVKDRIAVAMIDDAERRGVLKPGATVVEPTSGNTGIGLAMVCAVRGYRLI
                     LTMPDDYSVERQRLLARYGAEIHLTPAIEGMTGAVFAAQEMLREHAGYFMPSQFENPA
                     NPDVHRRTTALEILEATDDRVDAFVAGIGTGGTVTGVGEVLKQRLPSVRVIGVEPARS
                     PVLQGGKARPHGIQGIGASFVPAVLNRAVLDEVLLVRDEDATATSRRLAREEGLLVGI
                     SAGANVWAACQVASSMAPDQIVVTVLCDTGERYLSVPL"
     gene            complement(1642..2088)
                     /locus_tag="VFX87_05820"
     CDS             complement(1642..2088)
                     /locus_tag="VFX87_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX4994464.1"
                     /translation="MRISAKGEYAIKAMLDLALADGGGLRPIQDIAARQDIPQRYLEQ
                     VLLGLTRAGLLVSKRGSAGGYRLARAADQVTVGDVLRAVEGSMNGLEPTRRGRRGLGR
                     DPVSDLSELWREIDEAVSGVIDRITFEDLRKRAEERRSIARPMYHI"
     gene            complement(2085..>2509)
                     /locus_tag="VFX87_05825"
     CDS             complement(2085..>2509)
                     /locus_tag="VFX87_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX4994465.1"
                     /translation="PIMAYSAKYASAFYGPFREAADSAPQFGDRRAYQMDPANGIEAM
                     REVALDVDEGADIVMVKPALPYLDVVARVKADFGLPVAAYSVSGEYAMIRAAGQLGWL
                     DEERAMLEALTGIRRAGADIIITYFAKDAARLLEQGRV"
BASE COUNT          349 a          896 c          884 g          380 t
ORIGIN      
        1 gtcgatatgc gaatggtaga tgatggccac gcggaagcct tccgcctcgc gccggccgat
       61 ggtgagcaga tcgcgggggt cgatgtagta cgcggtgcgg gcgtcgcgcg gatggcgcgc
      121 cgggtccttt tgatgcaggt cgttctggat gttccggcag ggcaggagca gccggtccgc
      181 cgggcggtcg ccgcgcgcga gcacgacccc gcagcactcg ccgggatact cggcggcggc
      241 ctgggcctcg acccgggcgt actcctccgg agtcagcgcg gcgcggccgt ctttcacgct
      301 tccaggtgcg cttcggcgca cgcgccgccg gcccgcacgg cttgcccccc ggcgagggcg
      361 gcctgccccc cggcgagggc tggaatgatc gagacctcgt cgccgtcctt cagcgcggtg
      421 tcgagcccct tgagcgcctc gatggcctcg ctgttgacgt agatgttcac gtgatggtgg
      481 atctgaccct gctcgttgcg tacgagcccc ttgagccccg cgaactgcgc ttcgaggccg
      541 tcgagcagcc ccttgacgtc gggagcctcg agggagacgc ggtcccggtt gctggtggcg
      601 cggcggaacg gcgttggaat atagacctgc acgctcatgg atagggctcc tcagagaggc
      661 acgctgagat agcgttcccc agtatcgcac agcaccgtga cgacgatctg gtccggcgcc
      721 atgctcgacg cgacctggca ggcggcccac acgttggccc ccgccgagat ccctaccagc
      781 aggccctcct cccgggccag ccgccgcgag gtggcggtgg cgtcctcgtc gcgcacgagg
      841 agcacctcgt cgagcaccgc gcggttcagc accgcgggga cgaacgaggc gccgatgccc
      901 tggatgccgt gggggcgcgc cttgccgccc tggagcaccg gcgagcgcgc cggctccacg
      961 ccgatgaccc ggacggacgg cagccgctgc ttgagcacct cccccacgcc ggtcacggtg
     1021 ccgcccgtgc cgatgccggc cacgaacgcg tcgacgcgat cgtcggtggc ctcgaggatc
     1081 tccagcgcgg tggtgcggcg gtgcacatcc gggttggcgg gattctcgaa ctggctgggc
     1141 atgaagtagc cggcgtgctc ccgcagcatc tcctgcgccg cgaacaccgc ccccgtcatg
     1201 ccctcgatgg cgggcgtgag atggatctcg gcgccgtacc gcgcgagcag ccgctgccgc
     1261 tccacgctgt agtcgtcggg catggtgagg atgagccggt agccgcgcac cgcgcacacc
     1321 atggccagcc cgatcccggt attgccgctg gtgggctcca ccacggtggc gccgggcttg
     1381 agcacgccgc gtcgctcggc gtcgtcgatc atggccaccg cgatgcggtc cttcacgctg
     1441 ccgccgggat tgagcgattc catcttcgcc agcacggtgg cgcctccggg gcgcgggagc
     1501 tggttgagcc gcaccagcgg agttccgccc acgaggtcga gcaccgacgc ggccagccgc
     1561 ggccgccacg ttgccgtcgt cttcttctgg gtcatcagtc ccatccccat cgggaacgct
     1621 gtttcgccca caaaaaaagg gttagatgtg gtacatgggc cgggcgatgc tgcggcgctc
     1681 ctcggcgcgc ttccgcaggt cctcgaatgt gatccgatcg atgacgcccg acaccgcctc
     1741 gtcgatctcc cgccagagct cggagagatc ggacacggga tcccggccca ggccccgacg
     1801 gccgcgccgc gtcggctcga gcccgttcat cgagccctcc accgcgcgga ggacgtcgcc
     1861 caccgtgacc tggtcggcgg cccgcgccag gcgatagccc ccggcggagc cgcgcttgga
     1921 gacgaggagc ccggcccggg tgagccccag caacacctgc tcgagatagc gctggggaat
     1981 gtcctggcgc gcggcgatgt cctggatggg gcgcagcccg ccgccgtcgg ccagcgccag
     2041 atcgagcatc gccttgatgg cgtactcgcc cttggccgaa atcctcacac gcgcccctgc
     2101 tcgaggaggc gcgccgcgtc cttggcgaag taggtgatga tgatgtcggc gcccgcgcgg
     2161 cggatgccgg tgagggcctc cagcatcgcc cgctcctcgt cgagccagcc gagctggccc
     2221 gcggcgcgga tcatcgcgta ctcgccggac acggagtagg ccgccaccgg caggccgaag
     2281 tcggccttca cccgcgccac cacgtcgaga tagggcagcg cgggcttgac catcacgatg
     2341 tccgcgccct cgtccacgtc gagggcgact tcccgcatcg cctcgatgcc gttggcggga
     2401 tccatctggt aggcgcggcg gtcgccgaac tgcggcgccg agtccgcggc ttcgcggaac
     2461 gggccgtaga acgcggaggc gtacttggcg gaatacgcca tgatcggcg
//
LOCUS       DASUFA010000161         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_253297, whole genome shotgun sequence.
ACCESSION   DASUFA010000161 DASUFA010000000
VERSION     DASUFA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253297"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFX87_05830"
     CDS             complement(<1..956)
                     /locus_tag="VFX87_05830"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEX4994466.1"
                     /translation="MDILERRERGFCLTEVKSTTSVKEQHLPDVAVQTHVLRRCGLDV
                     GRMEVMHLNRACAYPDLSSLFTRADVTEPVEARLAAVPGELAAMTAMLAGPLPSVATG
                     PHCAKPYECPFTARCWPVLPPYHVSTLRAMQQRLLELDEQGYRSILELPEDLPLGPIA
                     DRQRRAVQAGRIVAEPTLGAALAAFTAPLAFLDFETVGLAIPVWEGCHPYDAVPVQFC
                     AYTPEGTGALAPHAWLADGPGDPRPALAERLLAACEGARSVVAYGAGFERDRIRELAA
                     ALPHLAARLEGLAARLVDLLPVVRNHVYHPDFGGSFSLKRVLP"
     gene            complement(1023..1280)
                     /locus_tag="VFX87_05835"
     CDS             complement(1023..1280)
                     /locus_tag="VFX87_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX4994467.1"
                     /translation="MTPPAEAAGPSRLSKSRAMAGLQCHKLLWWMTHEPAAAELEVDG
                     ARQAVFDQGHRVGALARTYVPGGVLVDLPHDAYEQRLEATR"
     gene            complement(1293..1541)
                     /locus_tag="VFX87_05840"
     CDS             complement(1293..1541)
                     /locus_tag="VFX87_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX4994468.1"
                     /translation="MGFIVSIIIGGIVGWLASLIMKTNAQMGLLANILVGVVGSVLGH
                     WAAGALGIAAFGSLGGFIISLLGAVILIAILRAMGFFR"
     gene            1671..2654
                     /locus_tag="VFX87_05845"
     CDS             1671..2654
                     /locus_tag="VFX87_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019954965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX4994469.1"
                     /translation="MSTQMTEPRMDLEQHLHMYRQMAKIRAFEEQVNELYKGAKMPGL
                     AHLYVGEEAVAVGVCEALKRDDFITSTHRGHGHCLAKGASVSRMFAELLGKEAGYCRG
                     KGGSMHIADPETGNLGANAIVGGSAGLATGAALSAKMRKSGQVAVCFFGDGALGQGLL
                     YEAMNMASLWKLPVIYVCENNLYGEYTPGNETIAGEILSRPRAMGVHAESVDGQDVQA
                     VHATMKRLVERARRGEGPAFLECKTYRYYGHHVGDVNREYRTRDEERDWMTNRDPLET
                     LSTRLMQQRLAEQSVFDRIQADVKTEIESGVQYALTAPFPSPGQVNEDVYA"
     gene            2641..3912
                     /locus_tag="VFX87_05850"
     CDS             2641..3912
                     /locus_tag="VFX87_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX4994470.1"
                     /translation="MSTPDGELRPRIDYKALVQDDRIHASLYTDPRIFADEMERIFHR
                     GWVFVGHASEIPGAGDYVTRRIGAQPVIMVRGRDGDVSVFVNRCRHRGTMLCPAERGH
                     ARTFACPYHGWTYDLDGALLGVPYPGGYAALDKREYGLEPAPRVSSYRGFVFASFAAT
                     GITLAEHLGAATRLIDRSCDLSPTGEIELTAGWVKHRGAANWKTLPENDSDGYHLGFV
                     HLALFKTVRMQYQRVVGDEKAIKAVVRDWGHGHIEIDWSPGYQGPFEWFGGAAGPLVA
                     DFVAALERRDGPELARQRIMQGPAHALIFPNLFLGETNIAIVEPVDVEECVHWHTPMF
                     LAGVPEFNGRLLRMAEAGMGPASFLMPEDLIIATRNQQGLHARTPEWLLLSRGLDREY
                     VDDQGRAVSHVTDETTNRALWRHYRAVMTEA"
     gene            3928..4419
                     /locus_tag="VFX87_05855"
     CDS             3928..4419
                     /locus_tag="VFX87_05855"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX4994471.1"
                     /translation="MVSDKDRQAVVEFLYREARLADEARYAEWLALWTDDAIYWVPAT
                     ADPDADPETRISHIYDNRARLETRIKLLQTGHRYSQEPASLMRRLISNIEVEDGRDGQ
                     LVAGSNFMLAELSIQAKAEMHWWVGRVTHHLRRVDGALRMKLKKVVLINAAEPLPNLS
                     FLI"
     gene            complement(4433..>4825)
                     /locus_tag="VFX87_05860"
     CDS             complement(4433..>4825)
                     /locus_tag="VFX87_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994472.1"
                     /translation="PDLIGDARYETPEARWRNRAEVDRLVESWTAERPKHDVMKVLGE
                     AGVPCGACLDTGEVLTDPHLLAREMIVEVEHPVRGRYVTVGNPIKLSASPTTIGPSPL
                     LGQHREEILAELGYTEDDIRVLAADGAI"
BASE COUNT          758 a         1720 c         1703 g          644 t
ORIGIN      
        1 ggcagcacgc gcttgagcga gaagctgccg ccgaagtccg gatgataaac gtgattgcgc
       61 accacgggca acaggtccac caggcgcgcc gccaggccct cgagccgggc ggccaggtgc
      121 ggcagcgccg ccgcgagctc ccggatgcgg tcacgctcga agcccgcgcc gtaggccacg
      181 acggaccggg cgccctcgca cgcggcgagg agacgttccg ccagcgccgg gcgcgggtct
      241 ccgggcccgt ccgcgagcca ggcgtgagga gccagggccc cggtcccctc cggcgtgtac
      301 gcgcagaatt gcaccgggac cgcgtcatag ggatggcagc cctcccacac cggaatggcg
      361 agcccgacgg tctcgaagtc gaggaaggcc agcggcgcgg tgaacgcggc gagtgccgcg
      421 cccagcgtcg gctccgccac gatccgcccc gcctgcacgg cccgtcgctg ccggtcggcg
      481 atcggcccga gcggcagatc ctcgggcagc tcgaggatgc tgcggtagcc ctgctcgtcc
      541 agctcgagca ggcgctgctg catggcgcgg agcgtgctga cgtggtaggg cggcagcacc
      601 ggccagcacc gcgcagtgaa cgggcactcg tagggctttg cgcagtgcgg gccggtggcc
      661 acgctcggca gcgggcccgc gagcatggcg gtcatggccg cgagctcgcc cgggaccgcg
      721 gccagccgcg cctcgaccgg ctcggtcacg tcggcccgcg tgaagaggct gctcaggtcc
      781 gggtacgcgc aggcgcggtt gagatgcatg acctccatgc ggcccacgtc gagcccgcag
      841 cggcgcagca cgtgggtctg caccgccacg tcgggcagat gctgctcctt cacgctggtg
      901 gtggacttga cctcggtcag gcagaagccg cgctcgcgcc gctcgaggat gtccaccgag
      961 acgaagacac cgtcggcgcg gaaggccgct tcatagacgg cgggggcgcc cccggcgagg
     1021 gcctagcggg ttgcctcgag ccgctgctcg taggcgtcgt ggggcaggtc cacgagcacg
     1081 ccgccgggca cgtaggtccg ggccagcgcg ccgacccggt ggccctggtc gaatacggcc
     1141 tggcgcgccc cgtcgacctc gagctcggcc gcggccggct cgtgcgtcat ccaccagagc
     1201 agcttgtgac actgcagccc cgccatcgcg cgggacttgg agaggcggga ggggccggcc
     1261 gcctcagccg gaggcgtcac caggcctccg gctcagcgga agaagcccat cgcccgcagg
     1321 atcgcgatca ggatcaccgc gccgagtagc gagatgatga agccgcccag cgaaccgaag
     1381 gccgcgatgc ccagggcccc cgcggcccag tggcccagga cggagcccac cacgccgacc
     1441 aggatgttcg cgagcaggcc catctgcgcg ttggtcttca tgatcaggct ggcgagccag
     1501 ccgacgatgc cgccgatgat gatggacacg atgaagccca tagcggtgtg ccctcccgtg
     1561 catgttgagc ggggcctcga cactgccgcc gcgacggtag cacgactgcc gcaggcccgg
     1621 ccggcaatgt atactcgcgc cacggagcac ctgccaaagg gggatcgacc atgtcgacgc
     1681 agatgaccga gccgcggatg gacctggagc agcacctcca catgtaccgg cagatggcca
     1741 agatccgcgc cttcgaggag caggtgaacg agctctacaa gggcgcgaag atgcccggcc
     1801 tggcccacct ctatgtcggc gaggaggcgg tcgcggtcgg ggtctgcgag gcgctcaagc
     1861 gcgacgactt catcacgagc acgcaccgcg gccacggcca ctgcctggcc aagggcgcct
     1921 cggtgagccg gatgttcgcc gagctgctcg gcaaggaagc gggctactgc cgcggcaagg
     1981 gcggctccat gcacatcgcc gatcccgaga ccggcaacct cggcgccaac gccatcgtgg
     2041 gcggctccgc gggcctcgcc accggcgccg cgctctccgc caagatgcgc aagagcggcc
     2101 aggtcgcggt gtgcttcttc ggcgacggcg cgctggggca gggcctgctc tacgaggcca
     2161 tgaacatggc ctcgctctgg aagctgccgg tcatctacgt ctgcgagaac aatctctacg
     2221 gcgagtacac ccccggcaac gagacgatcg cgggcgagat cctgtcccgc ccccgcgcga
     2281 tgggcgtcca cgccgagagc gtggacgggc aggacgtgca ggcggtccac gccacgatga
     2341 agcgcctggt cgaacgggcg cgccgcggcg agggccccgc tttcctcgag tgcaagacgt
     2401 accgatacta cggccaccac gtgggcgacg tgaaccggga gtaccgcacc cgcgacgaag
     2461 agcgggactg gatgaccaac cgcgatccgc tcgagacgct gtccacgcgg ctcatgcagc
     2521 agcggctcgc cgagcagtcc gtcttcgacc gcatccaggc ggacgtgaag accgagatcg
     2581 agagcggcgt gcagtacgcg ctgaccgccc cgttcccgag cccgggccaa gtgaacgagg
     2641 atgtctacgc ctgacggcga gctccgtccc cgcatcgact acaaggcgct ggtgcaggac
     2701 gatcgcatcc acgcctcgct ctacaccgat ccgcgcatct tcgccgacga gatggagcgg
     2761 atcttccatc gcggatgggt cttcgtcggc cacgccagcg agatccccgg cgcgggcgac
     2821 tacgtcaccc gccggatcgg cgcccagccg gtcatcatgg tccgcggccg cgacggtgac
     2881 gtctcggtct tcgtcaaccg ctgccggcac cgcggcacga tgctctgtcc ggccgagcgc
     2941 ggccacgccc gcacgttcgc ctgcccctac cacgggtgga cgtacgatct cgacggcgcg
     3001 ctcctgggcg tgccctatcc gggcggctac gcggcgctcg acaagcgcga gtacgggctc
     3061 gagccggccc cgcgggtgtc gagctaccgg ggattcgtct tcgcgtcgtt cgcggcgacg
     3121 ggtatcacgc tcgccgagca cctgggggcc gcgacccggc tgatcgaccg ctcctgcgac
     3181 ctctccccca cgggggagat cgagctgacc gcgggctggg tgaagcatcg cggcgcggcc
     3241 aactggaaga cgctgcccga gaacgacagc gacggctacc acctgggctt cgtgcacctc
     3301 gcgctcttca agacggtccg catgcagtac cagcgcgtgg tcggcgacga gaaggccatc
     3361 aaggcggtcg tgcgcgactg ggggcacggc cacatcgaga tcgactggtc gcccggctac
     3421 caggggccgt tcgagtggtt cggcggggcg gcgggaccgc tcgtcgccga cttcgtggcc
     3481 gcgctcgagc gccgggacgg gccggagctg gcgcggcagc gaatcatgca gggccccgcg
     3541 cacgccctca tctttcccaa cctgttcctc ggggagacca acatcgcgat cgtcgagccg
     3601 gtggacgtgg aggagtgcgt acactggcac accccgatgt tcctcgcggg cgtgcccgag
     3661 ttcaacgggc gcctgctgcg gatggccgag gcgggcatgg gccccgcctc cttcctgatg
     3721 ccggaggatc tcatcatcgc cacccgcaac cagcagggcc tgcacgcgcg cacgccggag
     3781 tggctcctgc tgagccgggg cctcgaccgc gagtacgtgg atgaccaggg ccgcgccgtc
     3841 tcccacgtca ccgacgagac gacgaaccgg gcgctctggc ggcactaccg cgcggtgatg
     3901 acggaggcgt agtcggcgaa gcgcccgatg gtcagcgaca aggaccggca ggccgtcgtc
     3961 gagtttctgt accgggaagc gcggctggcc gacgaggccc gctacgccga gtggctcgcg
     4021 ctctggaccg acgacgcgat ctactgggtg ccggccaccg ccgatccgga cgccgacccg
     4081 gagacgcgca tctcgcacat ctacgacaac cgcgcgcgcc tcgagacccg catcaagctg
     4141 ctccagaccg gccaccgcta cagccaggag cctgcgtccc tgatgcggcg gctcatctcc
     4201 aatatcgagg tggaggatgg gcgggacggc cagctcgtcg ccggctcgaa cttcatgctg
     4261 gccgagctgt ccatccaggc caaggccgag atgcactggt gggtggggcg cgtgacgcat
     4321 cacctgcggc gcgtggacgg cgcgctgcgc atgaagctca agaaggtcgt gctgatcaac
     4381 gcggccgagc cgctgccgaa cctctcgttt ctgatctgac gccggacggc gcctagatcg
     4441 cgccgtcggc ggcgagcacg cggatgtcgt cctcggtgta gcccagctcg gcgaggatct
     4501 cctcgcggtg ctggccgagc agcggagatg gcccgatcgt ggtcggcgag gcggagagct
     4561 tgatggggtt gccgaccgtc acgtaccgcc cgcgcaccgg gtgctcgacc tccacgatca
     4621 tctcgcgcgc gagcaggtgc gggtcggtca gcacctcgcc ggtgtcgaga caggctccgc
     4681 acggcacccc ggcctcgccc agcaccttca tgacgtcgtg cttcgggcgc tcggcggtcc
     4741 agctctccac caaccggtcc acctcggccc ggttccgcca gcgcgcctcc ggcgtctcgt
     4801 agcgcgcgtc gccgatcagg tccgg
//
LOCUS       DASUFA010000162         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_610443, whole genome shotgun sequence.
ACCESSION   DASUFA010000162 DASUFA010000000
VERSION     DASUFA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610443"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..954
                     /locus_tag="VFX87_05865"
     CDS             40..954
                     /locus_tag="VFX87_05865"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX4994473.1"
                     /translation="MVKPATSLLDRARGAILGHAAGNALGVPTEFLHTPEAIARRFPG
                     GVVDVVRADRPDSPWDDDVAMAVVVAEELLGPRPDPREILRRWVRWKESDGRGIGAWT
                     ATALEYFARHDAPIPDAHGNAGNGAVMRTIPVALATHRQPRALLHAACGIAALTHPDP
                     RCVWSAAAVAVACAQLLQGRRDFLPDVVEALRTNDAPAEVLDAVRRVPRERREDLVLT
                     GPAAGYTVLCMEAALWAAWHERSLESALAWLAGAGGDTDTNAAVAGGLLGARDGEGAI
                     PRRWVEAVARWEYLGELGERLVGGSGER"
     gene            complement(1038..1502)
                     /gene="queF"
                     /locus_tag="VFX87_05870"
                     /pseudo
     CDS             complement(1038..1502)
                     /gene="queF"
                     /locus_tag="VFX87_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
     gene            complement(1526..>2991)
                     /locus_tag="VFX87_05875"
     CDS             complement(1526..>2991)
                     /locus_tag="VFX87_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994474.1"
                     /translation="TSQDGSLTALLSAAQSIGVPQPLKLFGRSDQKKKYLPRLAKGAI
                     SAFALTETGVGSDPAGLATTAELSADGTHYVLNGEKLWTTNGTVAELMVVMARTGPKR
                     ITAFIVETAWEGVEVVHRLEFMGLKAIENGVMRFTNVKVPVENVLWGEGKGLKLALIT
                     LNTGRLTLPASCVAGAKRALEIARRWASERVQWGQPVGKHDAVAQKIGRMAADTFAME
                     AVSDLASLMADHGGYDIRLEAAMAKMWNTETTWRIVDDLVQIKGGRGYETADSLRRRG
                     DRPDPVERMMRDMRINLIFEGSSEIMRLFIAREAVDTHFSVAGDLVNPKAPAGVKAKA
                     LLKAIGFYAWWYPSQWFGWGRWPRYAEFGPLARHLRFVDRKSRKLARTVFHCMVRFGP
                     KLEKRQAVLGRLVEIGAELFAMSATVSRAHAMVRKNPGDRGPLELADVFCRQASRRVN
                     DKFEDVWFNDDSRTYRLAQGVLKNQYEWLEEGIVGNL"
BASE COUNT          381 a         1086 c         1082 g          442 t
ORIGIN      
        1 gactattctg cggccggaaa ctacccggaa tgccccccca tggtgaagcc cgccacgtcc
       61 ctcctcgacc gcgcccgcgg cgcgatcctc ggccacgccg ccgggaacgc cctgggggtg
      121 ccgaccgagt tcctccacac gcccgaggcc atcgcgcgcc gctttcccgg cggcgtcgtg
      181 gacgtggtcc gggcggaccg ccccgactcc ccctgggacg acgacgtcgc gatggcggtg
      241 gtcgtggcgg aggagctgct cgggccccgg ccggacccgc gggagatcct gcgccgctgg
      301 gtgcggtgga aggagtcgga cgggcggggc atcggggcgt ggaccgcgac cgccctggag
      361 tacttcgccc gccacgacgc cccgatcccc gacgcccacg ggaacgccgg caacggcgcc
      421 gtgatgcgca cgatccccgt ggcgctcgcc acccaccggc agccgcgggc cctgctgcat
      481 gccgcctgcg gcatcgccgc cctgacccac cccgacccgc gctgcgtctg gagtgccgcg
      541 gcggtggcgg tggcgtgcgc ccagctgctg caggggcgcc gggacttcct ccccgacgtg
      601 gtcgaggcgc tgcggacgaa cgacgccccg gcggaggtgc tcgacgcggt gcggcgcgtg
      661 ccgcgggagc ggcgggagga cctcgtgctc accggcccgg ccgcgggcta caccgtgctc
      721 tgcatggagg ccgccctctg ggccgcgtgg cacgagcggt ccctggagtc ggccctggcc
      781 tggctggcgg gcgccggggg cgacaccgac accaacgccg ccgtggccgg cgggctgctg
      841 ggcgcgcgcg acggcgaggg cgcgatcccg cgccgctggg tggaggcggt cgcgaggtgg
      901 gaatacctgg gggagctggg ggagcggctg gtggggggga gcggtgaacg gtaagcggtg
      961 agcggtaagc ggggcggcgg ggcccgctca ccgctccccg ctccccgctc ccctcccggg
     1021 agggcgacgg tgcaggtcgt ctcgaacccc ccccggacgt tgtacacgga ggtcacgctc
     1081 agctcccggg gctcgagggc ccggtggagg tcctcggcga tcttcgccgt ggcgtgctcc
     1141 tggaagatgc ccacggagcg atagctcgtc acgtagtact tgaggctctt cagctccacg
     1201 cacttcgcgc gcggcacgta gcggatcgtg agggtggcga agtcgggcag cccgctgtag
     1261 gggcagacgg cgctgaactc gcgcgtctcg gtcacgatct cctgccgcgg tccttcgtag
     1321 gggaaagtct ccagcacccg cacgtccacc gtctcgggtc cggtgaaggg cagggtccgg
     1381 ccttcggcga tgggcatcgg tggctccttg gctgggaggg aaaactggtc ggcaggacgg
     1441 caggacggca agacggcagg cgggcgcccg cgaatccgcc tcggggcgcg tcgacctgcc
     1501 atcctgccgt cctgccgacc tgccgtcaga ggttcccgac gatcccttcc tcgagccact
     1561 cgtactggtt cttgaggacg ccctgcgcca ggcggtaggt gcgggagtcg tcgttgaacc
     1621 agacgtcctc gaacttgtcg ttcacccgcc ggctggcctg ccggcagaag acgtcggcca
     1681 gctcgagcgg cccgcggtcg ccggggttct tcctcaccat cgcgtgcgcc cggctcacgg
     1741 tcgcggacat cgcgaagagc tcggccccga tctcgaccag ccggccgagc accgcctgtc
     1801 gcttctccag cttgggcccg aaccgcacca tgcagtggaa gaccgtccgc gcgagcttcc
     1861 gcgacttccg gtccacgaag cgcaggtgcc tcgccagcgg cccgaactcg gcgtagcgcg
     1921 gccaccggcc ccagccgaac cactggctcg ggtaccacca ggcgtagaag ccgatcgcct
     1981 tgagcagcgc cttcgccttg acgcccgcgg gcgccttcgg gttcaccagg tcgccggcga
     2041 cgctgaagtg ggtgtccacc gcctcgcggg cgatgaagag ccgcatgatc tcgctcgacc
     2101 cctcgaagat caggttgatg cgcatgtcgc gcatcatccg ctccaccggg tcgggccggt
     2161 cgccgcgccg gcgcaggctg tccgcggtct cgtacccgcg gccgcccttg atctgcacca
     2221 ggtcgtccac gatgcgccac gtggtctcgg tgttccacat cttggccatc gcggcctcga
     2281 ggcggatgtc gtagccgccg tggtcggcca tgagggaggc gaggtcgctc accgcctcca
     2341 tcgcgaaggt gtccgccgcc atccggccga tcttctgggc caccgcgtcg tgcttgccca
     2401 ccggctggcc ccactgcacc cgctccgacg cccagcggcg ggcgatctcg agcgcccgct
     2461 tggcgccggc cacgcagctg gccgggaggg tgagccgccc ggtgttgagg gtgatgagcg
     2521 cgagcttgag gcccttgccc tcgccccaga gcacgttctc caccggcacc ttgacgttgg
     2581 tgaatcgcat cacgccgttc tcgatggcct tgaggcccat gaactcgagc cggtgcacca
     2641 cctccacgcc ctcccacgcc gtctcgacga tgaacgccgt gatgcgcttc gggccggtgc
     2701 gggccatcac caccatcagc tcggcgaccg tgccgttggt ggtccagagc ttctcgccgt
     2761 tgagcacgta gtgggtgccg tcggccgaga gctccgcggt ggtggcgagc ccggcggggt
     2821 cggagccgac gccggtctcg gtgagcgcga aggcgctgat ggcgcccttc gcgagccgcg
     2881 gcaggtactt cttcttctgg tcggaccggc cgaagagctt gagcggctgc ggcacgccga
     2941 tggactgggc ggccgacagc agcgccgtca ggctcccgtc ctggctggtg a
//
LOCUS       DASUFA010000163         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_368585, whole genome shotgun sequence.
ACCESSION   DASUFA010000163 DASUFA010000000
VERSION     DASUFA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368585"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFX87_05880"
     CDS             complement(<1..972)
                     /locus_tag="VFX87_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994475.1"
                     /translation="MTGFTRRVSGYIWPHRRRILWALAQVCLISALELLKPWPLKLII
                     DSVLGGAPLPWGVAPDLGTGALLLGACAALVVIYAALGAANVLVNITTIRIGQRMVRD
                     LRSDLYAHLHRLSMAFHSRAQAGDLLYRVTADTFALQALTMNCLFPAVSALILLVGMA
                     VVMVQLDAALTLLALGVCPLLLLAIGRLNRQISAAAGEMRERESEVLGVVQRAMGAMR
                     VIQAFTREEDEHRRFMTASERSLAAGLRLYTLETFYSGVVNLVIALGTAAVVWVGARH
                     VLDSTLTVGSLVVFISYLAALYGPINNMFQVYGLAQSARVGVRRVLDV"
     gene            complement(969..>2637)
                     /locus_tag="VFX87_05885"
     CDS             complement(969..>2637)
                     /locus_tag="VFX87_05885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4994476.1"
                     /translation="RWYSTPGLPALAEYPKVSVVICAYNAEPTMEACLESLRNLRYPA
                     FEVVVVDDGSKDRTGAIADSFEGFHVIHQENKGLSAARNVGIAASLGEIVAFTDSDCV
                     VDPDWLHYLVATFLQTGLPAVGGPNLPPPEDSMVAACVAASPGGPMEVLLDDVEAEHI
                     PGCNMAFRREVLDEIGGFDPIYRSAGDDVDVCWRLQDRGYRIGFSPAAMVWHFRRNTM
                     KAYIGQQKGYGKAEALLYFRHPQRFNALGYSRWRGRIYGGISALFSLRRPIIYGGVFG
                     RGLFQTLYQPPSTVLSHLPFTLEWNLVGALLLAYGAMRGGWAWLGAAPIALSWACCLW
                     AAMRARVDAPACGVRGRLLIAVLTYFGPLLRSLERYRWWVRGLTAAEPSESERPMQAL
                     PWSWRERAFSAAFWTEDGLEKETILHGLVDALARRKYFVLVDQGWSEWDLEAHGGVWS
                     RGRIRVCTENHGGERRVLRVKCALRTSRLARGVLVGAAVMAGTGLMLGLPMVAAAGVA
                     AGVGTGAGLLRETVGLGRTLYDALQGVARRARLHYAPRLDVRDAEVK"
BASE COUNT          423 a          956 c          930 g          328 t
ORIGIN      
        1 cacgtcgagc acccgccgca cgccgacgcg tgcgctctgg gccaggccgt agacctggaa
       61 catgttgttg atgggcccgt agagcgcggc caggtagctg atgaagacga ccaggctacc
      121 caccgtgaga gtgctgtcga gcacgtgacg ggcgcccacc cacaccaccg cggcggtgcc
      181 cagggcgatg accaggttga ccacgcccga gtagaacgtc tccagggtgt agagccggag
      241 cccggcggcg aggcttcgct cgctcgcggt catgaaccgg cggtgctcgt cctcctcgcg
      301 cgtgaaggcc tggatcaccc gcatcgcgcc catcgcccgc tgcacgacgc cgagcacctc
      361 gctctcgcgc tcccgcatct cgcccgccgc cgcgctgatc tggcggttca gccggccgat
      421 ggcgagtagc agcagcgggc agacgcccag ggccagcagg gtgagcgccg cgtcgagctg
      481 caccatgacc acggccatcc cgaccagcag gatgagcgcg ctgaccgccg ggaagaggca
      541 gttcatggtg agcgcctgca gcgcgaaggt gtccgcggtc acccggtaca gcaagtcgcc
      601 cgcctgcgcc cggctgtgga aggccatcga gagccggtgc aggtgcgcgt acagatcgct
      661 gcgcaggtcc cgcaccatcc gttgcccgat gcggatggtg gtgatgttga cgagcacgtt
      721 ggccgcgccg agcgccgcgt agatgaccac gagggccgcg caggcgccca ggagcagcgc
      781 gccggtgccc agatcggggg ccacgcccca gggcaggggt gcgcccccga gcaccgagtc
      841 gatgatcagc ttcagcggcc agggcttgag cagctcgagc gcgctgatca ggcacacctg
      901 ggccagcgcc cacaggatgc gcctccggtg gggccagatg tagccggaga cgcgccgggt
      961 gaagccggtc acttcacctc ggcgtcgcgc acgtcgagcc gcggcgcgta gtgcagccgt
     1021 gcgcgtcgtg cgacgccctg gagcgcgtcg tagagcgtac gcccgagccc gaccgtctcg
     1081 cggagcaagc ccgcgcccgt ccccacgccg gcggccacgc ccgccgccgc caccatgggc
     1141 aggccgagca tcaggccggt gccggccatc accgccgcgc ccaccagcac gccgcgggcc
     1201 agccgcgagg tccgcagcgc gcacttgacc cgcagcacgc gccgctcgcc gccgtggttc
     1261 tcggtgcaca cccggatccg gccgcgagac cagaccccgc cgtgcgcctc gaggtcccac
     1321 tcgctccagc cctggtccac cagcacgaag tacttccgcc gcgccagcgc gtctacgagc
     1381 ccgtgcagga tcgtctcctt ctcgagcccg tcctcggtcc agaacgcggc cgagaacgcg
     1441 cgctcccgcc acgaccacgg cagcgcctgc atcggccgct cggactccga gggctcggcc
     1501 gcggtgagcc cgcgcaccca ccagcgatag cgctcgaggg agcgcaggag cggtccgaag
     1561 tacgttagga ccgcgatgag caggcgcccc cgcacgccgc acgccggcgc gtccacccgc
     1621 gcccgcatgg cggcccagag acaacaggcc cacgagagcg cgatcggcgc cgcgcccagc
     1681 cacgcccagc cgccgcgcat ggccccgtag gccaggagca gggccccgac cagattccac
     1741 tcgagggtga agggcaggtg ggagagcacg gtggacggcg gctggtagag cgtctggaag
     1801 aggccgcggc cgaacacgcc accgtagatg atgggccggc gcagggagaa gagggcggag
     1861 ataccgccgt agatccgccc gcgccaccgc gagtagccca gcgcgttgaa gcgctggggg
     1921 tggcggaagt agagcagcgc ctccgccttg ccgtagccct tctgctggcc gatgtaggcc
     1981 ttcatggtgt tgcgccggaa gtgccagacc atggcggccg ggctgaagcc gatgcggtag
     2041 ccgcggtcct ggaggcgcca gcacacgtcc acgtcgtcac ccgccgagcg gtagatcggg
     2101 tcgaagccgc cgatctcgtc caggacttca cgtcggaacg ccatgttgca gccggggatg
     2161 tgctcggcct cgacgtcgtc gagcagcacc tccatcggcc cgcccggcga ggcggccacg
     2221 cacgcggcga ccatcgaatc ttccggcggc ggcaggttgg gtccccccac cgcggggagc
     2281 ccggtttgca ggaaggtcgc gaccaggtag tgcagccagt ccggatccac cacgcagtcg
     2341 gagtcggtga aggcgacgat ctcgccgagc gaggccgcga tgccgacgtt gcgcgcggcg
     2401 ctgagcccct tgttctcctg gtggatgacg tggaagccct cgaagctgtc cgcgatggcg
     2461 ccggtgcggt ccttggagcc gtcgtccacc accaccacct cgaaggcggg atagcggagg
     2521 ttccgcaggg attcgagaca ggcctccatg gtcggctccg cgttgtaggc gcagatgacc
     2581 accgagacct tgggatactc ggcgagcgcg ggtagcccag gcgtgctgta ccagcgc
//
LOCUS       DASUFA010000164         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_147586, whole genome shotgun sequence.
ACCESSION   DASUFA010000164 DASUFA010000000
VERSION     DASUFA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147586"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VFX87_05890"
     CDS             <1..1043
                     /locus_tag="VFX87_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX4994477.1"
                     /translation="RRPLDAVVPVDDATTVVASAIGEALGLRVNPLAAAQATRNKLVM
                     RGRLARAGVPSPPFTTVGVDENPHAAAARMTYPCVLKPLVLSASRGVIRADDVTQFVA
                     AFERIREILRAADVAELGEGTDTILVEGFVPGGEVALEGLLEDGVLRTLALFDKPDPL
                     DGPFFEETIYVTPSRLPEATQARVAETTAAAARALGLSNGAVHCELRLRPAAAGLEPV
                     VLEIAARSIGGLCGRTLRFGTGMSLEELILRSALGRAVPSFERERAAAAVMMIPIPRG
                     GVLEEVGGLDEARAVPHIEDVTISIPKGQEIVPLPEGSRYLGFIFSRATTPAEAEAAL
                     REAHRHLRFVIR"
     gene            1107..1520
                     /locus_tag="VFX87_05895"
     CDS             1107..1520
                     /locus_tag="VFX87_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4994478.1"
                     /translation="MREFERAFNRQDVSALVACFTPDGSYHDTFFGGHAGADQLRAMF
                     ERMFREGRDYTWAMETVVETEECAAAEWTFAYVVSDAIPRSAGRRVSFRGMSVFEKRD
                     GRIAAYRESFDLGAALLQLGFSPEATVKTIRRYRL"
     gene            1532..2494
                     /locus_tag="VFX87_05900"
     CDS             1532..2494
                     /locus_tag="VFX87_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994479.1"
                     /translation="MSTYILRRLLLFVPTLLGASILIFVLMRLVPGDIAEILVYQTGS
                     ESSAIQQKQIQQIRRELGLDRPVVIQYLDWIGGAVRGDFGQSYVQRRPVMDILKERFP
                     RSMELAALTMLIAMAWAVPLGVVSAVRRNGPLDYLARLVSLSGLSLPLFFTGALILYG
                     LVRFFRWLPPLEFVSFTENPVENLKQLIWPALAQAYYISAPITRLTRSQMLEVIRQDY
                     VRTARAKGLAERAVVYRHALRNSLLPVVTFIGWWGGRLLGGLVIMEIIFVVPGMGSAL
                     IQAVSQRDYPTVQAMVFMMALVFLIVNLVVDLLYVWLDPRIRYA"
     gene            2491..>3291
                     /locus_tag="VFX87_05905"
     CDS             2491..>3291
                     /locus_tag="VFX87_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994480.1"
                     /translation="MRAAAARFVTGQPLGAAGALVVLVLIVVALFAPALAPHGPKDAA
                     FAPYVAPGREFPMGTDQLGRDLLSRVIWGARLSLYVGVVSVLFGITLGAVWGVVTGYL
                     GGAADAGSQRVVDCLMALPPIILALSLMAALGQSVDNVILALVILLTPTAARTVRSVA
                     LGVAATPYIEAAQAVGATRTRVIFRHLIPNCMASYIVLVTTNISYAIVVEASLSFIGA
                     GAPPDEPSWGGMLTAGIQALETAPWMVFFPGLAISLAVFGLNLLGDSIR"
BASE COUNT          409 a         1177 c         1172 g          533 t
ORIGIN      
        1 cccggcggcc cctcgacgcg gtggtgccgg tggacgacgc caccaccgtg gtggcctcgg
       61 cgatcggcga ggcgctcggc ctgcgcgtga acccgctggc cgcggcccag gccacgcgca
      121 acaagctcgt gatgcgcggg cggctcgcgc gggcgggggt gccctcgccg ccgttcacca
      181 cggtcggggt ggacgagaat ccgcacgcgg cggcggcgcg catgacgtac ccgtgtgtgc
      241 tcaagccgct ggtgctctcg gccagccgcg gcgtgatccg ggcggacgac gtcacgcagt
      301 tcgtcgccgc cttcgaacgg atccgcgaga tcctgcgcgc ggcggacgtg gcggagctgg
      361 gcgaggggac cgacaccatc ctggtggagg gcttcgtgcc ggggggcgag gtggcgctcg
      421 aaggcctgct ggaggacggg gtcctgcgca ccctggccct cttcgacaag cccgatccgc
      481 tcgacggtcc cttcttcgag gagaccatct acgtgacgcc gtcgcggctg cccgaggcca
      541 cccaggcccg ggtggcggag acgacggccg ccgccgcccg cgccctcggc ctctcgaacg
      601 gcgcggtgca ctgcgagctg cggctccgcc cggccgcggc cgggctcgag ccggtggtgc
      661 tggagatcgc ggcccgctcg atcggcggtc tctgcggccg caccctgcgc ttcggcaccg
      721 gcatgtcgct cgaggagctg attctacggt ccgccctcgg tcgggcggtg ccctcgttcg
      781 agcgcgagcg cgcggccgcg gcggtcatga tgatcccgat cccgcgcggc ggcgtgctgg
      841 aggaggtggg cgggctcgac gaggcccgcg cggtgccgca catcgaggac gtgacgatct
      901 cgatccccaa gggacaggag atcgtccccc tgccggaggg ctcgcgctat ctcggcttca
      961 tcttctcgcg cgcgaccacg ccggccgagg cggaggcggc cctgcgggaa gcccatcgcc
     1021 acctgcggtt cgtgatccgc tagactcggg ccgccggggg ccggtcgctc ggtgagaaga
     1081 atcaggggaa gcgcgccata agcatcgtgc gggagttcga gcgggccttc aaccgacagg
     1141 acgtctccgc cctggtagcc tgcttcacgc cggacggcag ctatcacgac accttcttcg
     1201 gcggacacgc cggggccgac cagctccgcg ccatgttcga gcggatgttc cgcgagggcc
     1261 gcgactacac ctgggcgatg gagacggtgg tggagacgga ggagtgcgcg gccgcggagt
     1321 ggaccttcgc ctacgtggtg agcgacgcga tcccgcgcag cgcggggcgc cgggtgagct
     1381 tccgcggcat gagcgtgttc gagaagcgcg acgggaggat cgcggcctac cgcgagtcct
     1441 tcgacctggg cgcggcgctg ctccagctcg gcttcagccc cgaggcgacc gtgaagacca
     1501 tccgccgcta ccgcctgtga ccggctgatc cgtgtcgacg tacatcctgc gccggctcct
     1561 gctgttcgtc cccaccctcc tcggcgcctc gatcctcatc ttcgtgctca tgcgcctggt
     1621 gccgggggac atcgcggaga tcctggtcta ccagaccggc tccgagtcga gcgcgatcca
     1681 gcaaaagcag atccagcaga tccgccgcga gctgggcctc gaccgtcccg tggtcatcca
     1741 gtacctcgac tggatcggcg gcgcggttcg tggcgacttc ggccagtcct atgtgcagcg
     1801 ccggccggtg atggacatcc tgaaggagcg cttcccgcgc tcgatggagc tggccgcgct
     1861 caccatgctg atcgcgatgg cgtgggcggt cccgctcggg gtggtctcgg cggtgcggcg
     1921 gaacggcccg ctcgactacc tggcccgcct ggtcagcctg agcgggctca gcctgcccct
     1981 gttcttcacc ggcgcgctca tcctgtacgg gctcgtgcgg ttcttccgct ggctgccgcc
     2041 gctcgagttc gtctcgttca ccgagaaccc ggtggagaac ctcaagcagc tcatctggcc
     2101 cgcgctggcc caggcctact acatcagcgc gcccatcacg cggctcaccc ggtcgcagat
     2161 gctcgaggtg atccgccagg actacgtgcg caccgcgcgg gccaagggcc tggccgagcg
     2221 tgcggtggtc taccgccacg cgctgcgcaa ctcgctgctg ccggtggtca ccttcatcgg
     2281 ctggtggggc gggcgcctcc tcggcgggct cgtgatcatg gagatcatct tcgtggtccc
     2341 gggcatgggc agcgcgctca tccaggcggt cagccagcgc gactacccca ccgtccaggc
     2401 catggtcttc atgatggccc tcgtcttcct gatcgtgaac ctcgtggtgg atcttctcta
     2461 cgtgtggctc gacccgcgca tccggtacgc atgagagccg cggccgcccg cttcgtgacc
     2521 ggccagcccc tcggcgcggc gggcgcgctc gtggtcctgg tgctcatcgt ggtggcgctg
     2581 ttcgcccccg cgctggcccc gcacgggccc aaggacgcgg ccttcgcccc gtacgtggcg
     2641 ccgggccgcg agttcccgat gggcaccgat cagctcggcc gcgacctcct gagccgcgtg
     2701 atctggggcg cgcgactctc gctctacgtg ggcgtggtct cggtgctgtt cggcatcacc
     2761 ctgggcgcgg tatggggcgt ggtgaccggg tacctgggcg gcgccgccga cgcggggagc
     2821 cagcgggtgg tggactgcct gatggcgctg ccgcccatca tcctggcgct ctcgctcatg
     2881 gccgcgctcg gccagtcggt ggacaacgtg atcctggcgc tggtgatcct gctcaccccc
     2941 accgcggccc ggacggtccg ctcggtcgcg ctcggagtgg cggcgacgcc gtacatcgag
     3001 gcggcgcagg cggtgggcgc cacgcggacc cgcgtcatct tccggcatct gatcccgaac
     3061 tgcatggcga gctacatcgt gctcgtgacc accaacatct cctacgccat cgtggtggag
     3121 gcctcgctca gcttcatcgg cgccggcgcg ccccccgacg agccgtcgtg gggcggcatg
     3181 ctcacggcgg ggatccaggc gctcgaaacc gcgccctgga tggtattctt cccgggactg
     3241 gccatcagcc tggccgtgtt cggtctcaac ctactcggcg actcgatccg c
//
LOCUS       DASUFA010000165         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_39482, whole genome shotgun sequence.
ACCESSION   DASUFA010000165 DASUFA010000000
VERSION     DASUFA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39482"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFX87_05910"
     CDS             complement(<1..526)
                     /locus_tag="VFX87_05910"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994481.1"
                     /translation="MRAYLLSRLGQTVLVVFLSLTAVFFLMRLGGDPVLLFLPMDIQA
                     KDLTEFRERLGFNDPVAVQYGRFVGRAVRGDFGESLRYKQDALGLVLERLPATLHLAV
                     AALGLTFLVALPVGVLSAVRRGSLFDLFGMGAAVLGQAVPGFWLGLMLIYLFSVKLGW
                     LPTGGTGSARHFVMP"
     gene            549..1721
                     /locus_tag="VFX87_05915"
     CDS             549..1721
                     /locus_tag="VFX87_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX4994482.1"
                     /translation="MSLARDLSVSAVTAGFVTVLVGFASSAVIVFQAAGAVGATPAQV
                     SSWMLALGLGMGLTCVLLSLRYRAPVVTAWSTPGAALLVTSAAGLTIEEATGAFLVSA
                     ALITIAGFSGWFERVMDRIPVAIASGMLAGVLLRFGLDVFLAMRTRFALAFGMFAAYL
                     VARRLAPRYAVIAALAVGVAVAVGQGLLRMDETRLALATPVWVTPRFTAGALIGVALP
                     LFVVTMASQNVPGVAVIRASGYAVPISPLIGWTGLANLGLAPFGGFALNLAAITAAIC
                     MGREAHEDPRRRYVAAMAAGAFYMVVGLFGATVVGLFAAFPRELVAAVAGLALFATIG
                     NGLAAALRDENQREPALVTFLVTASGVALSGVGSAFWGLVAGVVTLLILRVGRRRG"
     gene            1749..>2116
                     /locus_tag="VFX87_05920"
     CDS             1749..>2116
                     /locus_tag="VFX87_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4994483.1"
                     /translation="MDGQSRDNRSAVLPAVIVGLCLVVGLALGGYFVGKGAARFRSDV
                     RIVTVKGLVEREVKADQAVWTLGLRRASESLQDAHARISADRDAAVAFLRKQGFAEAE
                     IERQPTRTVDKLAREYGQPQA"
BASE COUNT          269 a          715 c          813 g          319 t
ORIGIN      
        1 acggcatcac gaagtggcgc gcgctgcccg tgcccccggt gggcagccag ccgagcttca
       61 ccgagaagag gtagatcagc atgaggccga gccagaagcc ggggacggcc tggcccagca
      121 ccgcggcgcc catgccgaag agatcgaaga gcgagccgcg acggaccgcc gagagcacgc
      181 cgaccggtaa cgcgaccagg aaggtgagcc cgagcgcggc gaccgccagg tggagggtgg
      241 cgggcagccg ctccaggacg aggccgaggg cgtcctgctt gtagcgcagg gactcgccga
      301 agtcgccccg caccgcgcgc ccgacgaagc ggccgtactg caccgccacc ggatcgttga
      361 agcccaggcg ctcgcggaac tcggtgaggt ccttggcctg gatgtccatg ggcaggaaca
      421 gcaggaccgg gtcgccgccc agccgcatga ggaagaagac ggcggtgagc gagaggaaca
      481 cgaccagcac ggtctgcccg agccgcgaca gcaggtaggc gcgcacgcgg ggagtatata
      541 gtggccgggt gtctctggcc cgggacctgt ccgtatcggc ggtgaccgcc ggcttcgtga
      601 ccgtgctggt ggggttcgcc agctcggcgg tgatcgtctt ccaggccgcg ggcgcggtcg
      661 gcgcgacccc ggcccaggtg agctcgtgga tgctcgcgct cggcctcggg atggggctga
      721 cctgcgtgct gctctcactc cgctaccggg cgcccgtggt caccgcgtgg tccactccgg
      781 gggcggccct gctcgtcacc agtgccgcgg gcctcaccat cgaggaggcc accggcgcct
      841 tcctcgtgtc ggccgcgctg atcacgatcg ccggcttctc cggctggttc gagcgggtga
      901 tggaccgcat cccggtggcg atcgcctccg gcatgctggc gggcgtgctg ctgcgcttcg
      961 ggctcgacgt gttcctggcc atgcggaccc gcttcgcgct ggccttcggc atgttcgcgg
     1021 cctacctggt ggcgcggcgg ctggctccgc gctacgcggt gatcgcggcg ctggcggtgg
     1081 gtgtggcggt ggccgtcggc cagggtctgc tgcggatgga cgagacgcgg ctggcgctgg
     1141 ccaccccggt ctgggtgacg ccgcgcttca ccgccggcgc actgatcgga gtggccctgc
     1201 cgctcttcgt cgtgaccatg gcgtcccaga acgtgcccgg ggtggcggtg atccgcgcct
     1261 ccggctacgc ggtgccgatc tcgccgctga tcggctggac gggcctcgcc aatctcgggc
     1321 tggccccgtt cgggggtttc gcgctgaacc tggccgccat caccgccgcg atctgcatgg
     1381 gccgcgaggc gcacgaggat ccgcggcgcc gctacgtggc ggccatggcc gccggcgcct
     1441 tctacatggt ggtcggcctc ttcggggcca ctgtcgtcgg tctcttcgca gcctttccgc
     1501 gcgagctggt cgcggcggtc gcggggctgg ccctcttcgc caccatcggc aacgggctgg
     1561 ccgccgcgct ccgcgacgag aatcaacgcg agccagcgct cgtcacgttc ctggtgaccg
     1621 cgtccggggt ggccctctcg ggcgtcggct cggcgttctg gggactggtg gcgggggtcg
     1681 ttacccttct gatactacgg gtgggccgcc gtcgcggcta ggcggcccgg gaggagcgta
     1741 tggcatccat ggacggtcag tcgagggaca accggtcggc ggtgctgccc gcggtcatcg
     1801 tggggctctg cctggtggtg ggtctggcgc tcggcgggta cttcgtcggc aagggcgccg
     1861 cgcgcttcag atcagacgtt cgaatagtca cggtgaaggg gctggtggag cgggaggtca
     1921 aggcggacca ggcggtctgg acgctcggcc tgcggcgggc cagcgagagc ctccaggacg
     1981 cgcacgcgcg catcagcgcg gaccgcgacg cggcggtggc cttcttgcgc aagcagggct
     2041 tcgcggaggc ggagatcgag cggcagccca cccgcaccgt ggacaagctc gcccgggagt
     2101 acggccagcc gcaggc
//
LOCUS       DASUFA010000166         3835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1625296, whole genome shotgun sequence.
ACCESSION   DASUFA010000166 DASUFA010000000
VERSION     DASUFA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625296"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFX87_05925"
     CDS             <1..370
                     /locus_tag="VFX87_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4994484.1"
                     /translation="LATGAAVACPDRKVVCLEGDGSGMYTLQALWTQAREGLDVTTVV
                     FANRTYAILRHELTGVRAGTPGPKAADMLDIGRPDLDWVSLARGMGVPASRATTAEEF
                     NKQFAAGLAAPGPYLIEAVL"
     gene            complement(367..2268)
                     /locus_tag="VFX87_05930"
     CDS             complement(367..2268)
                     /locus_tag="VFX87_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEX4994485.1"
                     /translation="MASTVHPLDPLSAAEIEHAWEIVRAERAPGPRVRVIFIMLHEPA
                     KKVVLAHRPGDAVERAAFVVLVDSAAGKTYEAVVSLSRGRVLSWEHVPGAQPAIVLDE
                     FVECEAAVRADPRWQAAMRRRGVTDFGLAMVDSWSAGNFGFAQDEGRRLVRALTWVRR
                     HAQDNGYARPVANLLTVVDLNAMAVIAVEDGGVVPLPPEDGNYSPEAAGTRPELKPVE
                     IRQPEGPSFELAGHELRWQRWRMRIGFTPREGLVIHTVAYEDQGRERPVLYRASIADM
                     VVPYGDPRPTYFHRNAFDVGEYGIGTLANALQNGCDCLGEIRYLDAVVNDGQGRPVTL
                     ANAICLHEEDAGILWKHFDWRLGTTEVRRSRRLVVSSISTVGNYEYGFYWYFYLDGTI
                     QLEVKLTGVISNGTERPGEKPRWGEMVAPGVYGPIHQHFFNARLDMMVDGPDNSVYEV
                     NTVADAPGPENPHHNAFHTEATLLASEARAQRILDPLAGRFWKVVNPSVRNRLGEPVA
                     YKLMPGENVLPFAGAEAAVTKRAAFMTRHLWVTRHDPRERYAAGEYPNQHPGGAGLPT
                     YVRDDAPLENTDVVLWYTFGAHHIVRPEDWPVMPVTTIGFMLKPVGFFDRNPALDVPR
                     PMPHCCDSG"
     gene            complement(2288..2659)
                     /locus_tag="VFX87_05935"
     CDS             complement(2288..2659)
                     /locus_tag="VFX87_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4994486.1"
                     /translation="MRYMLLMYRDEKWWDAKPEQERTAIRQQAIDASEPLRKRGIFLA
                     GDPLLPTSTATTVRMRSGKPVATDGPFAETKEQLGGYTIVEVASLDEALAIAADHPLL
                     RVGGHSIEVRPIRELPPPAVE"
     gene            complement(2675..>3835)
                     /locus_tag="VFX87_05940"
     CDS             complement(2675..>3835)
                     /locus_tag="VFX87_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994487.1"
                     /translation="VGIGSMLSLGVFLQPMSEAMGWSRSGISLAAMLNFLCMGVGSFV
                     WGALSDRFGTRVVVLSGGILLGAGMVAASRAATLGQFQLLFGVIVGVAAGSFYAPMTA
                     TTTRWFTEHRSLAVALVSAGLGLGSMTVAPLAAWIITTYDWRVAMLAIGDLAWLLLIP
                     AALLVRNPPAPAPAAAAVPGVVAPREFTVAEALRTPQFAAIALTHFACCAAHSGPIFH
                     MVTNAIDHGVSAMAATTVLGAAGLASLSGRVICGLLADRLGAKRVLIVGLAIQAVAVG
                     LYLVTRELAGFYALALMFGLAYGGVMPLYAIVVREYFGARIMGAVFGAVAFASTLGMA
                     LGPWAGGWLYDSFGSYSWLFIGSFGIGLGAVAIAFAFRPPAPFRAAVPSVAH"
BASE COUNT          574 a         1390 c         1376 g          495 t
ORIGIN      
        1 cctggccacg ggcgccgccg tggcctgccc tgaccgcaag gtggtgtgcc tggagggcga
       61 cggcagcggc atgtacacgc tgcaggcgct ctggacccag gcgcgcgagg ggctcgacgt
      121 gaccaccgtg gtcttcgcca accggacgta cgcgatcctg cggcacgagc tgaccggcgt
      181 ccgcgcggga acgcccggcc ccaaggcggc cgacatgctc gacatcggcc gccccgatct
      241 cgactgggtc tcgctggcgc gcggcatggg cgtgcccgcg agccgcgcca ccaccgccga
      301 ggaattcaac aagcagttcg cggccggcct ggccgccccc ggcccctacc tgatcgaggc
      361 ggtgctctag ccgctgtcgc agcagtgcgg catcgggcgc gggacgtcga gcgccgggtt
      421 gcggtcgaag aagccgacgg gcttgagcat gaagccgatc gtggtcaccg gcatcaccgg
      481 ccagtcctcg gggcgcacga tgtggtgggc gccgaaggtg taccagagga cgacgtcggt
      541 gttctcgagc ggcgcgtcgt cccgcacgta ggtcggcaac cccgcgccgc cggggtgctg
      601 gttcgggtac tccccggccg cgtagcgctc gcgcggatcg tggcgcgtga cccagaggtg
      661 ccgggtcatg aaggcggcgc gcttggtgac ggcagcctcc gcgcccgcga acggcagcac
      721 gttctcgccc ggcatcagct tgtaggcgac cggctcgccg agccggttcc gcaccgaggg
      781 gttcacgacc ttccagaagc gcccggcgag cggatcgagg atccgctggg cccgggcctc
      841 cgaggccagc agcgtcgcct cggtgtggaa ggcgttgtgg tgcgggttct cgggcccggg
      901 cgcgtccgcg acggtgttga cctcgtagac cgagttgtcg ggcccgtcca ccatcatgtc
      961 gagccgggcg ttgaagaagt gctggtgaat gggcccgtag actccgggcg cgaccatctc
     1021 gccccaccgc ggcttctcac cgggccgctc ggtgccgttc gagatgacgc cggtcagctt
     1081 gacttccaac tggatcgtgc cgtcgaggta gaagtaccag tagaagccgt actcgtagtt
     1141 gcccaccgtc gagatggacg acaccaccag gcggcgtgag cggcgcacct cggtcgtccc
     1201 caggcgccag tcgaagtgct tccagaggat gcccgcgtcc tcctcgtgga gacagatcgc
     1261 gttcgccagg gtcacggggc ggccctggcc gtcgttgacc accgcgtcga ggtagcggat
     1321 ctcgccgagg cagtcgcagc cgttctgcag cgcgttggcc agcgtgccga tgccatactc
     1381 gcccacgtcg aacgcgttgc ggtggaagta ggtcgggcgc ggatcgccgt agggcaccac
     1441 catgtcggcg atcgaggcgc ggtagaggac cggccgctcg cgtccctggt cctcgtaggc
     1501 cacggtgtgg atcacgaggc cctcgcgggg cgtgaacccg atccgcatcc gccagcgctg
     1561 ccagcgcagc tcgtggcccg cgagctcgaa gctcggcccc tccggctggc ggatctcgac
     1621 cggcttgagc tcgggccgcg tgcccgccgc ctccggcgag tagttgccgt cctccggagg
     1681 cagcgggacg acgccgccgt cctccaccgc gatcacggcc atcgcgttca ggtccacgac
     1741 ggtcagcagg ttggccaccg ggcgggcgta gccgttgtcc tgcgcgtgcc ggcgcaccca
     1801 cgtgagcgcg cgcaccaatc gccgcccctc gtcctgcgcg aagccgaagt tgcccgccga
     1861 ccagctgtcc accatggcga ggccgaagtc ggtgaccccg cgccggcgca tggccgcctg
     1921 ccagcgcggg tcggcgcgga cggccgcctc gcactcgacg aactcgtcga gcacgatggc
     1981 gggctgggcg cccggcacgt gctcccacga gagcacgcgg cctcgggaga gcgacaccac
     2041 cgcctcgtag gtcttccccg ccgcggagtc caccagcacc acgaacgcgg cccgttccac
     2101 cgcatcaccc ggccggtgcg cgagcacgac cttcttggcc ggctcgtgga gcatgatgaa
     2161 gatgacccgc acccgtggcc ccggcgcgcg ctcggcccgg acgatctccc acgcgtgctc
     2221 gatctccgcc gcgctcaacg gatccagggg gtgcacggtc gacgccatgg gtcgtctcct
     2281 ccgcagccta ctcgaccgcc gggggcggca gctcccggat cggccgcacc tcgatcgagt
     2341 ggccgcccac ccgcagcaac gggtgatcgg ccgcgatcgc gagcgcctcg tcgaggctcg
     2401 cgacctcgac gatcgtgtag ccgcccagct gctccttggt ctcggcgaac ggcccgtccg
     2461 tggcgaccgg cttgccgctc cgcatccgca ccgtggtcgc ggtgctggtg ggcagcagcg
     2521 gatcgccggc caggaagatg ccgcgcttgc ggagcggttc ggaggcatcg atcgcctgct
     2581 ggcggatcgc ggtccgctcc tgctcgggct tcgcgtccca ccacttctcg tcccggtaca
     2641 tcagcagcat gtagcgcatg tcggtcggat cccttcagtg cgccacgctc ggcaccgcgg
     2701 cccggaacgg ggccgggggc cggaacgcga acgcgatggc caccgccccg aggccgatgc
     2761 cgaacgagcc gatgaagagc caggagtagc tgccgaacga atcgtagagc cagccgccgg
     2821 cccacggccc gagcgccatg cccagggtgg acgcgaaggc gaccgcgccg aagacggcgc
     2881 ccatgatccg ggccccgaag tactcgcgca cgacgatggc gtacagcggc atcacgcccc
     2941 cgtaggcgag gccgaacatg agggccagcg cgtagaaccc ggcgagctcg cgcgtgacca
     3001 ggtagaggcc caccgcgacc gcctggatgg cgagccccac gatcagcacg cgcttggccc
     3061 ccaggcggtc ggcgaggagc ccgcagatca cgcggccgct cagcgaggcc aggcccgccg
     3121 cgcccagcac ggtggtggcg gccatcgcgg agacgccgtg gtcgatcgcg ttggtgacca
     3181 tgtggaagat cgggcccgag tgcgcggcgc agcacgcgaa gtgggtgagg gcgatggccg
     3241 cgaactgcgg cgtgcgcagg gcctccgcca ccgtgaactc gcgcggcgcg acgacgccgg
     3301 gcaccgcggc cgcggcgggc gccggcgcgg gcgggttgcg cacgagcagc gcggcgggaa
     3361 tgagcaggag ccacgcgagg tcgccgatcg ccaacatcgc gacgcgccag tcgtaggtcg
     3421 tgatgatcca ggccgcgagg ggcgccaccg tcatcgagcc caggccgagc cccgccgaga
     3481 cgagcgcgac cgccaggctg cgatgctcgg tgaaccagcg ggtggtcgtc gcggtcatgg
     3541 gcgcgtagaa gctcccggcc gccacgccca cgatcacccc gaacaggagc tggaactggc
     3601 cgagggtggc cgcccggctc gcggccacca tgcccgcgcc cagcaggatg ccgccggaga
     3661 gcaccacgac gcgggtgccg aaccggtcgg agagcgcccc ccagacgaac gacccgacgc
     3721 ccatgcacag gaagttcagc atcgcggcca gcgagatgcc gctgcgggac catcccatcg
     3781 cctcggacat gggctgcagg aacacgccca gcgagagcat cgagccgatc ccgac
//
LOCUS       DASUFA010000167         7839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1407209, whole genome shotgun sequence.
ACCESSION   DASUFA010000167 DASUFA010000000
VERSION     DASUFA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407209"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VFX87_05945"
     CDS             complement(<1..1809)
                     /locus_tag="VFX87_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4994488.1"
                     /translation="MKSRTAYGAALLAVGLAFMCAYLAVPLGERSQLFLLLAAVVVSA
                     WYGGLGPGLLATAVAVVGHTVFMEAPYGDDLVRVLLFVLVAGAISSLAAGRRRAEDRA
                     RAQREEMAVTLASIGDAVVVTDGGERVTFMNAAAERLTGWTGDEARGNTLPVVFAIVS
                     EETRALVESPAARALRENRVAALPAGTLLARRGGDERAIDGSADPIRDRAGHTVGVVL
                     VFRDLTERRASERDQAEVLAREGAARRDAAALSAVGQALVQSLDAESVGRHIAEGIRA
                     LLGGTVSVLWELEHATNRLVAVATSGSGSPFPPGTRIPRDEALAGFAVTEGRPVSTSN
                     ILTDLRLTLAPETRALVEQSGHGSVLAVPLTIGGRITGVLAVGDVTGRAFGAEEIRRL
                     QDFANQAAIALEKARLFALETSRREQLEALATVQRDLSAELDLDRLLGLIVERAGRLF
                     EGAGFAYLFDEASQSLDGRLWGRAEPESEAPFPAAGGMVASCAGARRGLLVNDYPASP
                     YALPAVVRLGVRHAMAQPLVSRDRLLGVLVVARRVDVPFRPEDLAGFEGLAVQAAVAL
                     DNAILFVEARRQRREAEVLAELARSINTAQDVSTVLQ"
     gene            2221..3264
                     /locus_tag="VFX87_05950"
     CDS             2221..3264
                     /locus_tag="VFX87_05950"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4994489.1"
                     /translation="MGAQKARIGIIGAGAIGCVVGGLLAKAGHDVTLADPWPEHVAQI
                     KSGGLRLSGSCGEHVVPVRALTLGEFGQVREPFEAVFVAVKSYDTEWATQMAVDHLRN
                     PDGVVVDFQNGINDHRVAAVAGRERTLGCVILISAGMYEPAHAIRTDRGNAAFKIGEH
                     DGTDSPRARRIAEIMSEVAPAVVTTNLWGERWSKLALNCMLNPLAGLSGLGTAEVRSE
                     SVPRRVAAHLGAEVVRVGTTLGHRIEPILGIAAKRFADASGAADIEVLGREIAADAQS
                     RSGGRPSMLQDVMKGRRTEIDYLNGFVAVEGRRVGIPTPFNDAVVAAFHAHGVGQLKP
                     DPRNLEPLVRLLA"
     gene            3365..4087
                     /locus_tag="VFX87_05955"
     CDS             3365..4087
                     /locus_tag="VFX87_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4994490.1"
                     /translation="MELLFVLGVGLAAGTISGIVGFGSSIMLMPVLVIVFGPLHAVPI
                     MAIAAILANLSRVLIWWREVDWRAVAAYAVTGVPAAALGARTLLVLPPRLIECALGIF
                     FLLMIPARRWLAARGFRLRRPHLLALGAAIGFLTGIVVTTGPITAPIFLACGLVKGAF
                     IATEAAASLAVYLSKTAVFRHFGALPLPVITQGLITGASLMAGAWIAKRFVLRLHPDR
                     FRLLMDALMLISGLTMLYTAFR"
     gene            complement(4127..5257)
                     /locus_tag="VFX87_05960"
     CDS             complement(4127..5257)
                     /locus_tag="VFX87_05960"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994491.1"
                     /translation="MSAAPVLPLEGIRVVEVAQNLAGPMAAEILAHMGADVIKVERPE
                     GDDARKWGPPFWKGVSPGFLAVNANKRGISLDLKDPKAVAWLLDFLGTADVLVQNLRP
                     GSLDEMGLGPDTLLARYPRLIYCSVWAFGRSGPLKMKPGFEPMVQAFSGLMMMNGDEG
                     GPPTRIGTSILDYGTGMWTAMGALAGLVQRQRTGRGCVVDASLYETGLAWLKGHFASY
                     RASGEVPERHRTGSQRVVPFQGFETKTGTIIVAAGNDRLFAKLAAVVGHPEWATDERF
                     VTNAARVANKPALLALLEAIFVTRTKGEWIDLLEAGGVPCGPIHTLPEAVTQPQAEAI
                     GIIQRLPGDDYDVVALPLSFDGRRPPIRRAPPRIGEHDAEIK"
     gene            complement(5254..6147)
                     /locus_tag="VFX87_05965"
     CDS             complement(5254..6147)
                     /locus_tag="VFX87_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4994492.1"
                     /translation="MHTLTLGEVAISRITEIGRSAFPTTQMLPESDAGRIVQHHRWIK
                     PHFFDDVTGDLGSRIQTWIVRTPQHTVLVDTGVGNDKSRHETDLWNKRKGGYLDDLRA
                     AGVSPEQVDLVLCTHLHVDHVGWNTRLVDGRWVPTFPKARYVFPGEEWEFWKHENEAG
                     REKSGCIADSVVPVVEAGQAVMVDSAYQVDPWLSYEPWVGHTPGHVGVRVHTGAGDAV
                     FSGDLMHRVVQVAEPQWSSIFCYDRQQAAATRKAFVERHAASGDLVLPAHFPHPGRVV
                     RDGDGHRFEPVMLDAIAAGKA"
     gene            complement(6157..7227)
                     /locus_tag="VFX87_05970"
     CDS             complement(6157..7227)
                     /locus_tag="VFX87_05970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994493.1"
                     /translation="MNELQTILAETCTRLFTDHAATAVLEGAEKGEWPGALWQALEEN
                     GLTLPQVPEARGGAGGSWNDAFVVLMAAGRFAAPVPLAETMLAGSILAEAGLDAPLGP
                     MTVAPVHIDEGLTLARQGSGFTLSGSATRVPWGARAEHVVVVADAGGSPMVALVAGGR
                     AKTEADASLAREPRDTLTWSGAPVVAAAPRRGRLGKQPVWAGGALVRSAQMAGGLEFL
                     LAQSVKYVTERKQFGRPLAAFQTIQQNLALLAGHTAAAGMAAQQAFNVVERTGEGGDA
                     SFEIAVAKVRTGEAASLGAGIAHQAHGAIGFTYEHSLHFVTRRLWSWRAEFGAENHWS
                     VALGREVAARGAEALWPHMTSR"
     gene            complement(7293..>7839)
                     /locus_tag="VFX87_05975"
     CDS             complement(7293..>7839)
                     /locus_tag="VFX87_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994494.1"
                     /translation="ISIRPIIDLAGGHHFNEVHFQDCLVPDDMLVGKDGEGWKQVTTE
                     LGFERSGPERYLSSLQLVIELIREAGKEPPERAAALIGHFTAHLVTLRQMSLSIATML
                     QAGQNPNLEAAVMKDIGTTFEQEIPEAVHALTGAEPRIGSGTEFEKTLGYLVQHAPSF
                     SLRGGTREVLRGIIARGLGLR"
BASE COUNT         1063 a         2919 c         2747 g         1110 t
ORIGIN      
        1 ctggaggacg gtggacacgt cctgcgcggt gttgatggag cgagccagct cggcgagcac
       61 ctccgcctcg cgccgctgcc ggcgcgcctc cacgaagagg atcgcgttgt cgagcgccac
      121 cgcggcctgc accgcgaggc cctcgaaccc cgcgaggtcc tcgggccgga acgggacgtc
      181 cacgcgccgg gccaccacca gcacgcccag gagacggtcg cggctcacga gaggctgcgc
      241 catcgcgtgg cgaacgccca ggcggaccac cgcgggcagg gcgtacggtg aggccgggta
      301 gtcgttgacc agcagtccgc ggcgcgcgcc cgcgcagctc gccaccatcc cccccgccgc
      361 cgggaacggg gcctccgact ccggctcggc ccggccccat agtcggccgt cgagcgactg
      421 gctggcctcg tcgaagagat aggcgaagcc ggcgccttcg aagagccggc ccgcccgctc
      481 cacgatcagg ccgaggagcc ggtccagatc gagctcggcg ctgagatcgc gctgcacggt
      541 ggcgagggcc tcgagctgct cgcggcgcga cgtctcgagg gcgaagagcc gtgccttctc
      601 gagcgcgatg gcggcctggt tggcaaagtc ctgaagccgc cggatctcct ccgcgccgaa
      661 ggcgcgaccc gtcacgtcgc cgaccgcgag cacgcccgtg atccggccgc cgatcgtcag
      721 cggcaccgcg agcaccgagc cgtgcccgga ctgttcgacc agcgcgcggg tctcgggggc
      781 gagggtcagg cggaggtcgg tcaggatatt ggacgtcgag accgggcggc cctcggtcac
      841 cgcgaagccg gcgagcgctt cgtcccgcgg gatccgggtg cccgggggaa acgggctgcc
      901 cgagccggag gtcgcgaccg ccaccagacg gttcgtggcg tgctccagct cccacagcac
      961 ggacacggtc ccgccgagca gtgcgcgaat gccctcggcg atgtgccggc ccacgctctc
     1021 ggcgtcgagc gactgcacga gggcctggcc caccgccgag agcgccgccg cgtcgcgccg
     1081 ggccgccccc tcgcgcgcga ggacctccgc ctgatcgcgc tcgctcgcgc gccgctcggt
     1141 gagatcgcgg aagaccagca ccacgcccac cgtgtggccg gcgcgatccc ggatggggtc
     1201 ggcactgccg tcgatggcgc gctcgtcccc gccccgccgt gccagcaggg tgcccgcggg
     1261 cagcgccgcc acccgattct cacggagcgc gcgcgcggcc gggctctcga cgagcgcccg
     1321 cgtttcctcg ctcacgatgg cgaagaccac cgggagcgtg ttgccgcgcg cctcgtcccc
     1381 ggtccacccg gtgagacgct ccgccgccgc gttcatgaag gtgacgcgct cgcccccgtc
     1441 cgtgacgacg accgcgtccc cgatgctcgc gagcgtgacc gccatctctt cccgctgcgc
     1501 gcgcgcgcgg tcctcggcgc ggcggcggcc ggcggccagc gagctgatgg cgcccgccac
     1561 cagcacgaag aggaggacac ggacgaggtc gtcgccgtac ggcgcctcca tgaatacggt
     1621 gtgcccgacg acggcgaccg cggtggccag gaggcccgga ccgagcccgc cgtaccaggc
     1681 gctgacgacg acggcggcca ggagcaggaa gagctgcgac cgctcgccca ggggcaccgc
     1741 gaggtaggca cacatgaacg cgaggccgac cgccagcagg gcggcgccgt acgccgttcg
     1801 agacttcatc gagcgtgcac cacgttccgc tgcatcgcgt caggcacccg ccacatcata
     1861 cgcttcgggc aacggtccgg accgtgctca gaaggcaatg cccgtgcccg cggcccgccc
     1921 catgcaagtg gctgtttcgg cgggcttgcc ccggggatcc ccggcgcggc gcaatggcac
     1981 ccggtaagct cgactcggta aagctcgacc cggcgcaagg aggacgcgga tgagagacac
     2041 cggctggctg atcgtggcga tcgtgaccgg cgtgctggcg gtcatcggct ggatcgcgcg
     2101 cgagcgctgg accatgcgac gccgccgccg ctgacgccct tgcgctgccg cgcgcaagtc
     2161 cggtagccta cgcggggcca cgcggcggcg atgatcgagg ccgggggccg gaggaaatcc
     2221 atgggcgctc agaaggcgcg gatcgggatc atcggggccg gagccatcgg ctgcgtggtg
     2281 ggcggcctgc tggcgaaggc cggccacgac gtgacgctgg ccgatccctg gcccgagcac
     2341 gtggcgcaga tcaagagcgg cgggctccgg ctttccggga gctgcggcga gcacgtcgtg
     2401 cccgtccggg ccctgaccct gggcgagttc ggacaggtgc gcgagccgtt cgaggcggtg
     2461 ttcgtggcgg tgaagtccta cgacaccgag tgggccaccc agatggcggt ggaccacctg
     2521 cggaatcccg acggggtcgt ggtggatttc cagaacggca tcaacgacca tcgcgtggcc
     2581 gcggtggcgg ggcgcgagcg cacgctcggc tgcgtcatcc tcatcagcgc cggcatgtac
     2641 gagccggccc acgccatccg caccgatcgc ggcaacgccg ccttcaagat cggtgagcac
     2701 gacggcaccg actcgccgcg cgcccgccgg atcgccgaga tcatgagcga ggtcgcgccc
     2761 gcggtcgtga ccaccaacct gtggggcgag cgctggtcga agctcgcgct caactgcatg
     2821 ctgaacccgc tcgcgggcct cagcgggctc ggcaccgccg aggtgcgctc ggaatcggtg
     2881 ccgcgccggg tggccgccca cctcggggcc gaggtggtgc gcgtcggcac cacgctcggt
     2941 catcgcatcg agccgatcct ggggatcgca gccaagcggt tcgcggacgc ctccggggcg
     3001 gccgacatcg aggtcctcgg ccgtgagatc gcggccgacg cccagagccg gagcgggggc
     3061 cggccctcca tgctgcagga cgtgatgaag ggccgccgca ccgagatcga ctatctcaac
     3121 ggcttcgtcg ccgtcgaggg ccgccgcgtc gggattccca cgcccttcaa cgacgcggtc
     3181 gtggccgcct tccacgcgca cggcgtcggc cagctcaaac ccgatccccg caacctcgag
     3241 cctctcgtcc gcctcctcgc ctaggagtgc gctcgagtcg cttcgttcga gcgccggctt
     3301 cgccggcgca acccggcctg ggggaggctc ggagggggcc gtcgcggccc cctccgataa
     3361 cgtagtggag ctgctgttcg tgctgggggt cggtctggcc gccggcacga tcagcggcat
     3421 cgtgggcttc ggctcgtcca tcatgctgat gccggtgctg gtgatcgtgt tcgggccgct
     3481 ccacgcggtg ccgatcatgg cgatcgccgc gatcctggcc aacctctcgc gcgtgctgat
     3541 ctggtggcgc gaggtggact ggcgcgcggt cgcggcctac gccgtcaccg gcgtgcccgc
     3601 ggcggcgctc ggcgcgcgga cactgctggt gctgccgccg cgcctgatcg agtgcgcgct
     3661 cggcatcttc ttcctgctga tgatcccggc gcggcgctgg ctcgccgcgc gcggcttccg
     3721 cctgcgccgg ccgcacctgc tggcgctcgg cgcggcgatc ggcttcctca ccggcatcgt
     3781 ggtgaccacg ggcccgatca ccgcgccgat cttcctggcc tgcgggctcg tgaagggagc
     3841 cttcatcgcc accgaggccg cggcctcgct cgcggtctac ctgagcaaga ccgccgtgtt
     3901 ccgccacttc ggcgccctgc cgctcccggt catcacgcag gggctcatca ccggcgcctc
     3961 gctcatggcc ggcgcctgga tcgccaagcg cttcgtgctc cggctccacc ccgaccgctt
     4021 ccgcctgctg atggacgcgc tgatgctgat ctccggcctg acgatgctct acaccgcctt
     4081 tcgctgaacc cctctcccct ctggggagag ggcagggtga ggggcgctac ttgatctcgg
     4141 cgtcgtgctc gccgatgcgt gggggcgcgc ggcgaatcgg ggggcgccgg ccgtcgaagg
     4201 agagcggcag ggcgacgacg tcgtagtcgt caccggggag gcgctggatg atgccgatcg
     4261 cctcggcctg cggctgcgtg accgcctcgg gcagggtgtg gatggggccg cagggcacgc
     4321 cgcccgcctc cagcaggtcg atccactcgc ccttggtgcg cgtcacgaag atcgcctcca
     4381 gcagcgccag cagcgcgggc ttgttggcca cgcgcgcggc gttggtgacg aaacgctcgt
     4441 ccgtcgccca ctccgggtga cccacgacgg cggcgagctt ggcgaagagg cgatcgttgc
     4501 cggcggccac gatgatggta ccggtcttgg tctcgaagcc ctggaacggc accacgcgct
     4561 ggctgccggt gcgatggcgc tccggcacct cgccggaggc gcgatagctc gcgaagtggc
     4621 ccttgagcca cgcgagcccg gtctcgtaga gcgaggcgtc caccacgcag ccgcgcccgg
     4681 tgcgctggcg ctgcacgagc cccgcgagcg cgcccatcgc ggtccacatg ccggtgccgt
     4741 agtcgaggat ggaggtgccg atacgggtcg gcggcccgcc ctcgtcgccg ttcatcatca
     4801 tgaggcccga gaaggcctgc accatgggct cgaagcccgg cttcatcttg agcgggcccg
     4861 agcgcccgaa ggcccacacc gagcagtaga tgagccgcgg gtaacgcgcg agcagcgtgt
     4921 cggggccgag ccccatctca tcgagcgagc ccggccgcag gttctggacc agcacgtccg
     4981 ccgtccccag gaagtcgagc agccacgcca ccgccttcgg gtccttgaga tcgaggctga
     5041 tcccgcgctt gttggcgttg accgcgagga agcccggcga gacccccttc cagaacggcg
     5101 gcccccactt gcgcgcgtcg tcgccctcgg gccgctccac cttgatgacg tcggcgccca
     5161 tgtgcgccag gatctcggcg gccatcggtc ccgccaggtt ctgcgcgacc tcgacgacgc
     5221 gaatgccctc gagcgggagc accggcgcgg cgctcacgct ttccccgccg cgatcgcgtc
     5281 cagcatgacc ggctcgaagc gatgaccgtc gccgtcgcgc acaacgcgtc ccggatgcgg
     5341 gaagtgggcg ggcagcacca ggtcccccga ggcggcgtgg cgctccacga aggccttgcg
     5401 ggtggccgcc gcctgctggc ggtcatagca gaagatgctg ctccactggg gctcagccac
     5461 ctgcaccacc cggtgcatga ggtcgccgga gaagaccgcg tcgcccgcgc cggtgtgcac
     5521 gcgcacgccc acgtgcccgg gggtgtggcc cacccagggc tcgtacgaga gccacgggtc
     5581 cacctggtag gcgctgtcga ccatcaccgc ctggcccgcc tcgaccaccg gcaccacgct
     5641 gtccgcgatg cagcccgact tctcgcgccc cgcctcgttc tcgtgcttcc agaactccca
     5701 ctcctcgccc gggaagacgt accgggcctt cgggaacgtg ggcacccagc gcccgtccac
     5761 cagccgcgtg ttccagccca cgtgatccac gtgcagatgc gtgcacagca ccaggtccac
     5821 ctgctccggg gagacgccgg ccgcgcgcag atcgtcgagg tagccgccct tccgcttgtt
     5881 ccagagatcc gtctcgtgcc ggctcttgtc gttgcccacg cccgtgtcca ccagcaccgt
     5941 gtgctgcgga gtccgcacga tccaggtctg gatgcgcgag cccagatccc ccgtcacgtc
     6001 gtcgaagaag tgcggcttga tccagcggtg gtgctggacg atccgccccg cgtccgactc
     6061 cgggagcatc tgggtggtgg gaaaggccga gcggccgatc tcggtgattc gcgagatggc
     6121 cacttcgccg agcgtgaggg tgtgcatgtc tctctcctag cgtgacgtca tgtgcggcca
     6181 gagcgcctcc gcgccgcgcg ccgcgacctc gcgcccgagc gctaccgacc agtggttctc
     6241 ggcgccgaac tcggcccgcc acgaccagag ccgccgcgtg acgaagtgca gcgagtgctc
     6301 gtaggtgaat ccgatggcgc cgtgtgcctg gtgcgcgatg cccgcgccca gactcgccgc
     6361 ctcgccggtg cgcaccttgg ccaccgcgat ctcgaacgac gcgtcgccgc cctcgccggt
     6421 gcgctcgacc acgttgaagg cctgctgcgc cgccatcccg gcggccgcgg tgtgcccggc
     6481 gagcagcgcc aggttctgct ggatggtctg gaacgcggcg agcggccggc cgaactgctt
     6541 gcgctcggtc acgtacttca ccgactgggc cagcaggaac tcgaggccgc ccgccatctg
     6601 cgccgagcgc accagcgcgc cgcccgccca caccggctgc ttcccgagcc ggccacgccg
     6661 cggcgccgcc gccaccaccg gcgcgccgga ccacgtgagc gtgtcgcgcg gctcgcgcgc
     6721 gaggctcgcg tcggcctcgg tcttcgcgcg gccgccggcc accagcgcga ccatcggcga
     6781 tccgccggcg tcggcgacga ccaccacgtg ctcggcgcgc gcgccccacg gcacccgggt
     6841 cgcgctcccg ctcagggtga agccgctgcc ctgccgggcc agggtgaggc cctcatctat
     6901 atgcacgggc gccacggtca tggggccgag cggcgcgtcc aggcccgcct cggccaggat
     6961 cgagccggcc agcatggtct cggccagtgg caccggcgcc gcgaagcggc cggccgccat
     7021 caggaccacg aaggcgtcgt tccaggagcc gcccgccccg ccgcgggcct ccggcacctg
     7081 cggcagcgtg aggccattct cctccagggc ctgccagagg gcgcccggcc attcgccctt
     7141 ctccgcgccc tccagcaccg cggtcgcggc atgatcggtg aagaggcgcg tgcacgtctc
     7201 ggcgagaatc gtctgcagct cgttcatggc gggcatgacc cccttacttc ttttcttgta
     7261 ttcgctcggc tcggcttcgg cttcacctcg ccctacctca gcccgagacc gcgggcgatg
     7321 atccctctca agacctctcg cgttccccct ctcaacgaga acgacggcgc gtgctggacc
     7381 aggtagccca gcgtcttctc gaactcggtc ccgctgccga tgcgcggctc ggcgccggtg
     7441 agcgcgtgca ccgcctccgg gatctcctgc tcgaaggtgg tgccgatgtc cttcatcacc
     7501 gcggcctcca ggttcgggtt ctgcccggcc tgcagcatgg tggcgatgga cagcgacatc
     7561 tgccgcagcg tcaccaggtg cgcggtgaag tgaccgatca gcgcagccgc gcgctcgggc
     7621 ggctccttcc cggcctcgcg gatcagctcg atcacgagct ggagcgacga gagatagcgc
     7681 tcggggccgc tgcgctcgaa tccgagctcg gtggtcacct gcttccagcc ctcgccgtcc
     7741 ttgccgacca gcatgtcgtc gggcaccagg cagtcctgga agtgcacctc gttgaagtgg
     7801 tggccgccgg ccaggtcgat gatcggccgg atcgagata
//
LOCUS       DASUFA010000168         7120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1529035, whole genome shotgun sequence.
ACCESSION   DASUFA010000168 DASUFA010000000
VERSION     DASUFA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529035"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..891
                     /locus_tag="VFX87_05980"
     CDS             130..891
                     /locus_tag="VFX87_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994495.1"
                     /translation="MNEFLGLDGKVAVVTGGASGLGQAVARGLVREGARVVLGDIDKA
                     GLERTAAELGDRVRWQRTDVARRDDIAALFRLCDEAFGPAEILVTCAGVISSAPLAAM
                     TEDEWDRVLAINTKGTFFCIQEAVKRMVPQKRGRIITIGSDSVARGGGRVATAAYTAS
                     KGAVVALTRNIARDLLGTGVSVNCVNPGPIDSPMHAPLTAEQRARVVGSIPLGWMARP
                     EEIANGVLFLASSRAASFMYGTNLNIDGGTQMGTS"
     gene            896..2554
                     /locus_tag="VFX87_05985"
     CDS             896..2554
                     /locus_tag="VFX87_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4994496.1"
                     /translation="MRGKQVFMESLIAHGVEYIFGNPGTTESPILDALLDYPQLRYIV
                     ALHEGVALGAASYYAQTSGKLAVVNVHVAPGLGNALGMLYNAFKARAPLVVTAGQQDT
                     RLRLRGPVLGHDLVAMAAPLTKWSVQVERADEFALIMHRALKIATDPPAGPVFVALPI
                     DVLEQETDLPPLDPGRLYRAPEPEPAGVRAAAELLRGARRPAIVVGDDAASAAAEVAA
                     LAAQLGAGVWCEGIRAHQVMPTAHPNFRLGLPFDAVAIREALDGADVVLLVGGPFFEE
                     VWYAPGSPLPPGAAAIQVESAPERLAHNLPVSVGLVSHPRPALSALRAAIEREADPAF
                     REAAARRNDALRALKAQDAQAQRARAAKRWDHAPISVPRLMAEIEAALPADAIVVDES
                     ITASIELARTVQFERSGDYVGARGGGIGQALPGALGVKLAHPDRPVVALSGDGSAMYS
                     IQALWTAAHHDLAVVFVILNNREYRILKHNMDTYRQRFGAKPDRGYPNMDLVSPDLGF
                     VDLARGLGVEAVRVTGPGELRPALEKALGARRPFLLDVAIEGRA"
     gene            complement(2556..3365)
                     /locus_tag="VFX87_05990"
     CDS             complement(2556..3365)
                     /locus_tag="VFX87_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX4994497.1"
                     /translation="MSARRVAVFAAMALVALVTLPVRAAHETPRCAAPDELTRFRVAL
                     PNTTRAIRQGRALVIVAIGSSSTEGVGASDPAHAYPAVLAEELRRRWPRLEVTVINKG
                     VSGEMISEVLPRFERDVLPYRPQLVIWQTGSHAALGAADVEAYAAAVREGIGRLKAAR
                     MDVVLMDPQFAPRVLARPIHARVVDSIGMAARDMKVAVFRRFAVMQHWVSSGQYKMDD
                     VVSRDGLHLNDLSYGCIARLLADSLATAARAVPPSEEGSAAPAGAGEPAPR"
     gene            complement(3362..4144)
                     /locus_tag="VFX87_05995"
     CDS             complement(3362..4144)
                     /locus_tag="VFX87_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX4994498.1"
                     /translation="MRPLPGPRRRAVALIAAAALALAAGPPAVLAEEPVCPTPEGFAA
                     FETPLPKTAKALAAGSEVVIVTLGGTSSLGTAAGGPGFAWPSRLAAALAGRFPAARVK
                     VVNLAMPRQTAREAADRLPREVLPLKPTLVIWETGTMEAVRGISVDDFRDTVQAGIDE
                     LHTAGIEVVLMNPQFSPDADAVINFAPYLGAVRELSDANDVPVFHRYGLMRHWAEGGV
                     LDLRTRESEKRRRLAARLYECIGRAMADFVSRGGAPTPGGRP"
     gene            complement(4144..5301)
                     /locus_tag="VFX87_06000"
     CDS             complement(4144..5301)
                     /locus_tag="VFX87_06000"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX4994499.1"
                     /translation="MNAGRDLRLDVFRGLALFSIFVDHIPNNIVAQFTLQSVMFADAA
                     EVFILISGYTAGMVYGRAMERQGFLIAGLRVGHRVWQLYVAHVFLFAIFMATMAYTVD
                     ILNKSLYAEEFQAADFLSEPGVAVVKALTLQFQPAYMDILPLYIVLLGAFPFVLAGFR
                     FWPAGVLIASLGLWLAAQIDPRLALSAYPGPDRVWYFNPFGWQALFFVGAWLGWRGDR
                     GALSWLDRRWLLYLAAGFALAVFVIRFSWTLHGFSYRIPALASGKALWLFLSKEDLGL
                     LRFASVLAVALLVARLIAPQAAFLTGRLAWPFVICGRHSLHIFCLGILLSVVGHLVLS
                     EFFGGVAMQLAISAAGIGVMIAVAALMEWFSAAKGGAGDRVAERAPLAGGE"
     gene            5603..6619
                     /locus_tag="VFX87_06005"
     CDS             5603..6619
                     /locus_tag="VFX87_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994500.1"
                     /translation="MADAAFVSCGGAGAPQGRRREVVVSGKRVKTVDVHAHCAVPAAM
                     ALMGRKLETEALLMGNTQERLRAMDAQGIDVEALSINPYWYAADRDVATELIRIQNET
                     LAEICAAQPDRFVAFASVALQHPDLAAEQLEEGVKRYGLRGAGLGGSVEGLELSDPKF
                     HPFWAKAETLGVLVFIHPQGIAELERTGRLAGNGLLTNTIGNPLETTIALSHLIFEGT
                     LDRFPGLKICAAHGGGYLPSYAARSNAVQTTFPHRMTGTLKKRPTEYLRQLYFDSIVF
                     TPEALRHLAVEAGPGQIVMGTDYPFPWTQTSVDHILGTPELSDADKIAMLGGTAMALL
                     GIKG"
     gene            6661..6852
                     /locus_tag="VFX87_06010"
     CDS             6661..6852
                     /locus_tag="VFX87_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994501.1"
                     /translation="MLRLVIVLLTATLAALGLIVGVVVALGVAWIAVALPVLLLLGLA
                     AGLVALRRRVWPRPGDPPD"
     gene            complement(6902..>7120)
                     /locus_tag="VFX87_06015"
     CDS             complement(6902..>7120)
                     /locus_tag="VFX87_06015"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4994502.1"
                     /translation="FGAVKALVEHAGGTLDNVVKITTYVTDVRRRMDFRLVRDEFFGV
                     RGPASTMVEVSSLSHPDYLIEIEAIAIL"
BASE COUNT         1083 a         2564 c         2554 g          919 t
ORIGIN      
        1 ggccggcgcc tgatctccga gtccgagggc atcaaggcca cgatcgtggc gggcatcccg
       61 ctctacgagg agggccggca caccggcgcc atgccgggcc gcatcctgcg cagctacgag
      121 tagcgggcga tgaacgagtt cctcgggctg gacggcaagg tcgcggtggt gaccgggggc
      181 gcgagcgggc tcggccaggc ggtggcccgc ggcctggtgc gggagggcgc gcgcgtcgtg
      241 ctcggcgaca tcgacaaggc ggggctcgag cggaccgccg ccgagctcgg cgaccgggtc
      301 cggtggcagc gcaccgacgt cgcccggcgc gacgacatcg ccgccctgtt ccgcctgtgt
      361 gacgaggcgt tcgggccggc cgagatcctc gtcacctgcg ccggcgtcat cagctcggcc
      421 ccgctcgcgg ccatgaccga ggacgagtgg gaccgcgtgc tggccatcaa cacgaagggg
      481 accttcttct gcatccagga ggcggtcaag cggatggtgc cgcagaagcg cggccgcatc
      541 atcacgatcg gctccgacag cgtggcgcgc ggcggggggc gcgtcgccac cgcggcctac
      601 accgcctcga agggggcggt ggtcgcgctg acccgcaaca tcgcccgcga cctgctcggc
      661 accggcgtca gcgtcaactg cgtcaacccg gggccgatcg attcgccgat gcacgccccg
      721 ctcaccgccg agcagcgggc gcgcgtggtg ggcagcatcc cgctcggctg gatggcccgg
      781 cccgaggaga tcgccaacgg cgtgctgttc ctggcctcga gccgggccgc cagcttcatg
      841 tacgggacca acctgaacat cgacggcggc acccagatgg gcacgagctg accgcatgcg
      901 cggcaagcag gtcttcatgg agagcctgat cgcccacggg gtggagtaca tcttcggcaa
      961 ccccggcacc accgagagcc ccatcctgga cgccctgctc gactacccgc agctgcgcta
     1021 catcgtggcc ctgcacgagg gcgtggccct cggagccgcg agctactacg cccagacgag
     1081 cgggaagctc gcggtcgtca acgtgcacgt ggcgccgggc ctcggcaacg cgctcggcat
     1141 gctctacaac gccttcaagg cccgggcgcc gctggtcgtg accgccgggc agcaggacac
     1201 ccgcctgcgc ctgcgcgggc cggtgctcgg tcacgacctg gtggcgatgg cggcgccgct
     1261 caccaagtgg agcgtccagg tcgagcgcgc cgacgagttc gcgctgatca tgcaccgcgc
     1321 gctcaagatc gccaccgatc cgcccgcggg cccggtcttc gtcgcgctgc cgatcgatgt
     1381 gctcgagcag gagaccgacc tccccccgtt ggaccccggc cggctctacc gggcgcccga
     1441 gcccgagccc gccggggtcc gggcggccgc ggagctgctg cggggcgccc ggcggccggc
     1501 catcgtggtc ggggacgacg ccgcgagcgc ggcggccgag gtggcggcgc tggccgcgca
     1561 gctcggcgcg ggcgtctggt gcgaaggaat ccgggcgcac caggtgatgc cgaccgccca
     1621 cccgaacttc cgcctcggcc tgcccttcga cgcggtcgcc atccgcgagg cgctggacgg
     1681 cgcggacgtc gtcctcctcg tcggcgggcc gttcttcgag gaggtctggt acgcgcccgg
     1741 ctccccgctc ccgccgggcg ccgccgccat ccaggtggag tcggcccccg agcgcctggc
     1801 gcataacctc ccggtgagcg tcgggctggt gagccacccg cggcccgcgc tgtcggcgct
     1861 ccgggctgcg atcgagcgcg aggccgaccc cgccttccgc gaggcggcgg cccggcggaa
     1921 cgacgccctc cgcgcgctga aggcccagga cgcgcaggcc cagcgggccc gggcggccaa
     1981 gcgctgggat cacgcgccga tctcggtgcc gcgcctgatg gccgagatcg aagcggcgct
     2041 gcccgcagac gccatcgtcg tggacgaatc catcaccgcc agcatcgagc tggcccgcac
     2101 ggttcagttc gagcggtcgg gcgactacgt cggcgcgcgg ggcggcggca tcggccaggc
     2161 cctgcccggg gcgctcggcg tgaagctggc ccaccccgat cgcccggtgg tcgcgctgtc
     2221 cggggatggc tcggcgatgt acagcatcca ggcgctctgg accgccgcac accacgacct
     2281 cgcggtggtc ttcgtgatcc tgaacaaccg cgagtaccgc atcctcaagc acaacatgga
     2341 tacgtaccgc cagcgcttcg gcgccaagcc ggatcgcggc tacccgaaca tggacctcgt
     2401 ctcgcccgat ctcggcttcg tcgacctcgc gcgcggcctg ggcgtggagg ccgtgcgggt
     2461 gaccgggccg ggcgagctgc gacccgcgct ggagaaggcg ctcggcgccc gccggccctt
     2521 cctgctggac gtcgccatcg aggggcgcgc ctgagctagc ggggcgcggg ctcgccggcg
     2581 ccggcgggag cggccgagcc ctcctcggac ggggggactg cccgggccgc ggtggcgagg
     2641 ctgtcggcca gcaggcgcgc gatgcagccg tagctcaggt cgttcaggtg caggccgtcg
     2701 cgcgagacga cgtcgtccat cttgtactgg ccgctcgaca cccagtgctg catcaccgcg
     2761 aaccggcgga acaccgccac cttcatgtcg cgggcggcca tgccgatcga gtccacgacg
     2821 cgcgcgtgga tggggcgcgc gagcacgcgc ggggcgaatt gcggatccat cagcaccacg
     2881 tccatgcgcg ccgccttgag acggccgatt ccctcgcgga ccgcggccgc gtaggcctcg
     2941 acgtcggccg ccccgagcgc ggcgtggctc ccggtctgcc agatgacgag ctggggccgg
     3001 tagggcagca cgtcgcgctc gaagcgcggc agcacctccg agatcatctc gccgctgacc
     3061 cccttgttga tcacggtgac ctcgaggcgc ggccagcggc gccgcagctc ctcggcgagc
     3121 acggcggggt aggcgtgcgc cggatcggag gcgccgactc cctcggtcga agaggagccg
     3181 atcgcgacga tgaccagcgc gcgcccctgc cggatggcgc gcgtggtgtt gggcagcgcc
     3241 acgcggaacc gggtcagctc gtccggcgcg gcgcaccgcg gcgtctcgtg cgcggcgcgg
     3301 accggcagcg tcacgagcgc gacgagcgcc atcgccgcaa agaccgccac gcgccgggcg
     3361 ctcacggccg acccccgggc gtcggcgcgc cgccgcggct gacgaaatcg gccatcgcgc
     3421 gaccgatgca ctcgtagagt cgcgccgcga gccggcggcg cttctcgctc tcccgtgtgc
     3481 gcaggtcgag cacgccaccc tcggcccagt gacgcatgag gccgtaacgg tggaataccg
     3541 gcacgtcgtt ggcgtccgag agctcccgca ccgcgccgag atagggcgcg aagttgatca
     3601 ccgcgtcggc gtcgggagag aactgggggt tcatgaggac cacttcgatc ccggcggtgt
     3661 gcagctcgtc gatccccgcc tgcaccgtgt cgcggaagtc atccacgctg atcccgcgaa
     3721 ccgcctccat cgtgccggtc tcccagatca ccagcgtcgg tttcagcggc aggacctcgc
     3781 ggggcagccg atcggcggcc tcccgcgccg tctggcgcgg catcgcgagg ttgacgacct
     3841 tgacccgcgc ggcgggaaac cgccccgcga gcgccgcagc caggcgggaa ggccaggcga
     3901 agccgggccc gcccgccgcg gtaccgaggc tcgaggttcc gccgagggtg acgatgacga
     3961 cctccgagcc cgcggcgagc gccttcgcgg tcttcggaag cggtgtctcg aacgccgcga
     4021 agccctcggg ggtggggcac accggctcct cggcgaggac cgccgggggc ccggccgcca
     4081 gcgcgagggc cgccgccgcg atcagagcga ccgcgcggcg acgcggcccc gggagtggtc
     4141 tcatcactct cccccggcca gcggcgcccg ctccgcaacc cggtcgccgg cgccgccctt
     4201 cgcggcggag aaccattcca tgagcgccgc gaccgcgatc atgaccccga tgccggccgc
     4261 ggagatcgcg agctgcatgg cgacgccgcc aaagaactcg ctgagcacca gatggcccac
     4321 caccgagagc aggataccga ggcagaagat gtggagcgag tggcgtccgc agatgacgaa
     4381 gggccacgcc agccgaccgg tcaggaaggc cgcctgggga gcgatcagcc gcgccacgag
     4441 cagggcgacc gcgagcacac tggcgaagcg aagcaggccg agatcctcct tgctcaggaa
     4501 cagccacaag gccttgccgc tcgccagggc ggggatccgg taggagaacc cgtggagcgt
     4561 ccagctgaag cggatcacga acacggccag cgcgaagccc gcggccaggt acaacagcca
     4621 gcgccggtcg agccaggaca gggcgccgcg gtcaccgcgc cacccgagcc aggccccgac
     4681 gaagaacagc gcctgccagc cgaacgggtt gaagtaccac acccggtccg gccccggata
     4741 ggccgacagc gcgagccgcg ggtcgatctg ggcggccagc cacagcccga gcgaggcgat
     4801 cagcacgccc gccggccaga agcgaaatcc ggccagcacg aaggggaagg cccccagcag
     4861 gacgatgtag agcggcagga tatccatgta ggccggctgg aactgcagcg tcagtgcctt
     4921 gaccaccgcc acgcccggct cgctcaggaa gtcggcggcc tggaactcct cggcgtagag
     4981 cgacttgttc aggatatcga cggtgtacgc catcgtcgcc atgaagatcg cgaacaggaa
     5041 gacgtgggcg acgtagagct gccacacgcg gtggccgacc cggaggcccg cgatcaggaa
     5101 gccctggcgt tccatggccc ggccgtacac catgcccgcg gtgtagccgg agatcaggat
     5161 gaacacctcg gccgcgtcgg cgaacatcac cgactggagg gtgaactggg ccacgatgtt
     5221 gttcgggatg tggtcgacga agatggagaa gagggcgagg ccgcggaaga catcgagccg
     5281 caggtcgcgc ccggcattca tcaccgagac catcttacaa cgaccgcccg cacgagcatc
     5341 tttgccacgc tcatctcgcg gttctcccct cggccacccg gggccgctct tgccacgacc
     5401 tcccgatgat gctcttggcg caggtcgtcc accacgcctt ccttatgggc tagaatcctg
     5461 cggcgggtat agcaggaacc tcagggacgg aggccatcgc catgacgaca cgcggggagt
     5521 ttctccagat cggggccggc gcgcgcgccg gtatcgcctt gggtgggaac gcgatgaagc
     5581 ggctcgggat ccgagcctag ccgtggccga cgcggcgttc gtctcgtgcg gcggggccgg
     5641 ggccccgcaa gggcggcggc gggaggtggt cgtcagcggc aagcgcgtga agaccgtgga
     5701 cgtgcacgcg cactgcgcgg tgcctgcagc gatggcgctg atgggacgca agctggagac
     5761 cgaggccctc ctcatgggca atacgcagga gcgcctccgc gcgatggacg cgcagggcat
     5821 cgacgtggag gcgctcagca tcaaccccta ctggtacgcg gcggaccggg acgtcgccac
     5881 ggagctgatc cggatccaga acgagacgct ggccgagatc tgcgcggccc agccagaccg
     5941 gttcgtcgcg ttcgcgagcg tggccctgca gcatccggac ctggcggccg agcagctcga
     6001 ggagggcgtc aagcggtacg gcctgcgcgg cgccggcctc ggcggcagcg tggagggtct
     6061 cgagctgagc gacccgaaat ttcatccctt ctgggccaag gccgagacgc tgggggtcct
     6121 cgtgttcatt caccctcagg gcatcgccga gctggagcgc accggccggc tcgcgggcaa
     6181 cgggctcctc accaacacca tcggcaaccc cctcgagacg actatcgcgc tctcgcatct
     6241 catcttcgag ggcacgctcg accgcttccc cgggctgaag atctgcgccg cccacggcgg
     6301 cggctacttg ccctcctacg ccgcgcggtc caacgcggtg cagacgacct tcccccatcg
     6361 catgaccggg acgctcaaga agcggccgac ggagtacctg cgccagctct acttcgactc
     6421 gatcgtgttc acccccgagg ccctgcgcca cctggcggtg gaggcggggc caggccagat
     6481 cgtcatgggc accgactatc cgttcccgtg gacgcaaacg tcggtggacc acatcctggg
     6541 cacgccggag ctgagcgatg ccgacaagat cgcgatgctg ggcggcaccg cgatggcgct
     6601 gctggggatc aaggggtagc gtcacccgta cgcgggtgac ctcgtagcgt tccggccgcc
     6661 atgctccgac tggtgatcgt gctcctgacg gccactctcg ctgccctcgg cttgatcgtc
     6721 ggggtggtcg tcgcgctcgg ggtggcgtgg atcgccgtcg ccttgcccgt cttgctcctc
     6781 ctcggcctcg ccgcggggct ggtggcgctc cgacgtcggg tgtggccgcg acccggcgac
     6841 ccacccgact gagccgggcc gcccggccct cccgggcgaa cgagtcgggg aggatatccg
     6901 cctacaggat ggcgatcgcc tcgatctcga tcaggtagtc ggggtgcgac agcgagctga
     6961 cctcgaccat cgtcgaggcc ggcccgcgca cgccgaagaa ctcgtcgcgg accaggcgga
     7021 agtccatgcg gcggcggaca tcggtcacat aggtggtgat cttgaccacg ttgtcgagcg
     7081 tcccaccggc gtgctcgacc agcgccttca ccgcgccgaa
//
LOCUS       DASUFA010000169         8146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1281562, whole genome shotgun sequence.
ACCESSION   DASUFA010000169 DASUFA010000000
VERSION     DASUFA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281562"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFX87_06020"
     CDS             complement(<1..185)
                     /locus_tag="VFX87_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994503.1"
                     /translation="MVFISVVVGVLIHRARTPRAIPTEAAASDADYRLKQVRLQETAR
                     DGSRWQLDAEYSETFEER"
     gene            complement(283..795)
                     /locus_tag="VFX87_06025"
     CDS             complement(283..795)
                     /locus_tag="VFX87_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX4994504.1"
                     /translation="MRAAPRASRIRLLVLDVDGVLTDGGLLFSAAGEETKRFHVHDGL
                     AMVAARRAGLQIAILSSRASAAVTRRMAELGVGEVHQGVADKARALDALLERLGLAAG
                     EVAMMGDDLPDLPAMARAGLALAPANAAAEVKRAAHWVARRRGGDGAVREAVEMLLKA
                     RRAWPPRSEG"
     gene            complement(792..1775)
                     /locus_tag="VFX87_06030"
     CDS             complement(792..1775)
                     /locus_tag="VFX87_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX4994505.1"
                     /translation="MDFRIERARILSLAERVLRLEAESVLALCPRLDDRFVAAVEGLH
                     RCRGRVIVTGMGKSGLIGRKIAATLASTGTPAYFLHPAEGVHGDLGMVARGDVVLALS
                     NSGETDEVLAVLPALKRLGIPLILLTGAPGSTLARQADIVVDVGVAEEACPMNLAPTS
                     STTAALAMGDALAMVLLDLRGLGPGDYAALHPRGSLGWKALFKVSDLMHTGEALPLVS
                     EQATMKEAIEEMSGKGLGMTTVVDAGGRLAGVVTDGDLRRQQLAHGAALLERRVAECM
                     TTGPKIIGADELAAKALAMMEGRITSLVIVDAAGRPAGVIHLHDILRAKIV"
     gene            complement(1775..2623)
                     /gene="kdsA"
                     /locus_tag="VFX87_06035"
     CDS             complement(1775..2623)
                     /gene="kdsA"
                     /locus_tag="VFX87_06035"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX4994506.1"
                     /translation="MTPTRAVRIGSVTIGGGAPLALIGGPCAIENEKHALMMAERLQR
                     VTADAGVPFIYKSSYDKANRSSIHSYRGPGLTEGLRILQKVKDETGLAVLSDVHAVTE
                     VAPAAQVLDVLQVPAFLCRQTDLIVACARSGRPVNVKKGQFVAPRDMVNVVEKVRASG
                     SEDLLLTERGTSFGYNNLIVDFRGLPIMRAFGYPVVFDATHSVQLPGGQGDRSGGERQ
                     YVRALARAAVAVGVDALFMEIHEDPDRTLPDGRPLSDGPNMLRLDDLPRLLDELRAIA
                     AGPARA"
     gene            complement(2620..4230)
                     /locus_tag="VFX87_06040"
     CDS             complement(2620..4230)
                     /locus_tag="VFX87_06040"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX4994507.1"
                     /translation="MTTRFIFVTGGVVSSLGKGLAAASIGSLLEARGFRVTLQKMDPY
                     INVDAGTMSPYQHGEVYVTDDGGETDLDLGHYERFTSARMTRDNNVTTGKVYGTVIEK
                     ERRGDYLGRTVQVIPHITDQIKASIRKVAAGVDVVIIEIGGTVGDIEGLPFLEAVRQF
                     KKDVGRDNVIYVHLTLVPYIQAAQELKTKATQHSVKELRAIGIQPDVLLCRTAKDHPL
                     TPSIKSKIALFCDVEEEAVIAARDVDTIYEVPIAFHDQGLDESIIKLLGLPSRPIDLS
                     RWEEIVRRIKAPRRRCRIGVVGKYIEVKDSYKSLYEALAHGGIAHEAAVEVVWVDAER
                     IEREGLAGHVAGLDGILVPGGFGDRGIEGKIQAIRYAREQRVPYFGICLGMQCAVIEY
                     ARHVCGLEGANSTEFNPTVAHNVIDLLPEQRHVQDKGGTMRLGLYPVLLSEGTAASRA
                     YGQGLIEERHRHRYEVNNDYLARLEKNGLRISGLWAEKQLVEIIELPDHPWFVAGQFH
                     PEFRSRPWEPHPLFAAFIKAALDHQGGA"
     gene            complement(4232..5956)
                     /locus_tag="VFX87_06045"
     CDS             complement(4232..5956)
                     /locus_tag="VFX87_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994508.1"
                     /translation="MAKAGHVYPQVEPGIAALMDPRVLTCGPGVSVARALARARESGA
                     TVVLLGPGRAARVSELARAAAWGLDRRLAREVAWRGLPSVAAGASEIDVRRRLRAGTP
                     LVLVRRGSRVIGAVDRDRVDLTPPESSVLALFERAGEAKPRPSASPGPPPLNDARIWL
                     LQVAGKIGEGLGAPAFAVGGFVRDLLAGAAPPDVDLVVEGDGVAFARRLAEEIGGTVL
                     VHRSFGTASIEGGRAPAGAGLDGVALGRVDVASARREHYPEAGALPEVEPAGLLEDLR
                     RRDFSVNALALALSPDRFGRLLDPLGGQADVRRRRLRALHPLSFVEDPTRVFRAARYA
                     SRLGLRPDAATLAAIRLAVERPGYAALSGQRLWREIELAGAEPRPRLVFERLVRWKAT
                     SLWNLEGASSRFLAAAERLGRWAKSAGVVVDPAELALLALTLGRPAQVVERSLDRLAL
                     AGEPRARLEAGALAGPLARRLDTRGLPPSVVGELLDGAPESAALAAWLRGGARARRRI
                     QWYLAHGRAVRPRLTGRDLLALGVPGGPRVGAALGMLRRHRLDGGAGSVAEERELVKE
                     WLTSGKEA"
     gene            complement(5958..6944)
                     /gene="xerD"
                     /locus_tag="VFX87_06050"
     CDS             complement(5958..6944)
                     /gene="xerD"
                     /locus_tag="VFX87_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX4994509.1"
                     /translation="MRSRPGASAERGTAGVVSEAAALIDEYLGGLQIERGAALNTLAA
                     YRRDLKGFETFLQREHRAIGDVGVTDLSRYLGGLRRRGLGSRSIARHLSAVRGLYRFL
                     LDTGRVPRDPTQHLDSPRPARRLPRTLSMADAAALVEAPDTSRPDGLRDRALLELLYA
                     SGLRASEALGLRIEDVNFKAGYVMVIGKGDRQRLVPAGARALDWVRRYLTTIRPRQVR
                     RECPALFLNRSGGAMSRQALWGLIRRAAQRAGLRAAVSPHTLRHSFASHLLERGADLR
                     SVQAMLGHVDISTTQIYTHLPSSVVHDMYRKFHPRAARVARVARARGLERAG"
     gene            complement(7088..>8146)
                     /gene="murJ"
                     /locus_tag="VFX87_06055"
     CDS             complement(7088..>8146)
                     /gene="murJ"
                     /locus_tag="VFX87_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX4994510.1"
                     /translation="GPAVLNLAMIAAVVGLASRLTTPIMSLAIGVLVGGLGQLLVQLP
                     ELRRIGVPLRPSLEWRHPAVARIARRLWPAVFALAAVQITVVVNTLLASLLPTGTVSY
                     LYYADRVMEFPLGVFGIALATATLPSMSAQAARREFPALRATLEFALRLSAFVAVPAT
                     VGLLLLGGPIIQVLFQRGEFTAGDASLTAQALAGYAVGLPAFSATRIAAQTFYALGDT
                     RTPVIVGFVSVAVNVALALVLMWPLRHTGLALASSLSSYVNVIGLCWLLHRRLGGPRG
                     SEIWGSLGRTVVATAALALWCAWVEGFLAVVPEPVVLGLARMVLALVGGVAVYVAMSA
                     AIRAPELRALLGMLHARR"
BASE COUNT         1207 a         3010 c         2916 g         1013 t
ORIGIN      
        1 cgctcctcga aggtctcgga gtactcggca tcgagctgcc agcgggagcc atcccgggcc
       61 gtttcctgaa ggcgaacctg cttgagccgg taatccgcgt ccgaggcggc ggcctccgtg
      121 ggaatcgccc ggggcgtccg ggcccggtgg atgagcacgc ccaccaccac ggatatgaat
      181 accacgacga gcgtcagaat aatgagaggg gccttcgtca tagcaatctc catgccacgc
      241 tagcacagtc tttacagccg tggcaaatga agaaggcgcc gctcagccct cggagcgagg
      301 cggccaagca cggcgggcct ttaggagcat ctcgaccgcc tcgcgcacgg cgccgtcccc
      361 accccgccgg cgcgccaccc agtgcgcggc ccgcttgacc tcggccgcgg cgttggcggg
      421 cgccagcgcc aggccggcgc gggccatcgc gggcagatcc ggcaggtcgt cgcccatcat
      481 cgcgacctcc ccggcggcca ggccgagccg ctcgaggagc gcgtccaggg ccctcgcctt
      541 gtcggccaca ccctggtgaa cctcgcccac cccgagctcg gccatgcggc gggtcaccgc
      601 ggcggacgcg cgtgaggaga ggatcgcgat ctgcagtccc gcgcggcgcg cggccaccat
      661 cgcgagcccg tcgtggacgt ggaagcgctt ggtctcctcg ccggcggcgg agaacagcag
      721 cccgccgtcc gtgagcacgc cgtccacgtc gaggacgagc agccggatgc gggatgcgcg
      781 gggagcggcc ctcacacgat cttggcgcgc aggatgtcgt ggaggtggat cacgcccgcg
      841 ggccgccccg ccgcgtccac gatcacgagg ctcgtgatgc ggccctccat catcgcgagc
      901 gccttcgcgg cgagctcgtc ggcgccgatg atcttgggcc cggtggtcat gcactcggcg
      961 acccggcgct cgagcagcgc ggccccgtgg gcgagctgct ggcgccgcag gtcgccgtcg
     1021 gtcacgaccc cggcgagccg gccgcccgcg tccaccaccg tggtcatccc gagacccttg
     1081 ccgctcatct cctcgatggc ctccttcatg gtggcctgct cggagaccag cggcaacgcc
     1141 tcgccggtgt gcatgaggtc gctgaccttg aagagcgcct tccagcccag gctgccgcgc
     1201 gggtggagcg ccgcgtagtc gccgggcccg aggccgcgca ggtcgagcag caccatcgcg
     1261 agcgcgtcgc ccatcgcgag cgcggcggtg gtgctcgagg tcggcgcgag gttcatggga
     1321 caggcctcct cggccacgcc cacgtccacc acgatgtcgg cctggcgcgc cagcgtggag
     1381 cccggcgcgc cggtgaggag gatcagcggg atgccgagcc gcttgagcgc gggcagcacc
     1441 gccagcacct cgtcggtctc gccggagttc gacagggcca gcacgacgtc cccgcgcgcc
     1501 accatgccaa gatcgccgtg cacgccctcg gcgggatgga ggaagtaggc gggcgtcccg
     1561 gtgctcgcga gggtcgctgc gatcttgcgc ccgatgaggc ccgacttgcc catgccggtg
     1621 acgatgacgc gcccccgaca ccggtggaga ccctccaccg cggccacgaa gcgatcgtcg
     1681 agccgcgggc agagggcaag cacgctctcc gcctcgagcc gcagcacacg ctcggccagg
     1741 ctcaggatgc gcgcccgctc gatccggaaa tccatcaggc gcgggccggg ccggccgcga
     1801 tcgcgcggag ctcgtcgagg agccggggca gatcgtccag gcggagcatg ttgggtccgt
     1861 cggagagcgg gcggccgtcc gggagcgtgc ggtccgggtc ctcgtggatc tccatgaaga
     1921 gcgcgtccac gcccaccgcc accgccgcgc gcgcgagggc ccgcacgtac tggcgctcgc
     1981 cgcccgagcg atccccctgc ccgcccggga gctgcaccga gtgggtcgcg tcgaacacga
     2041 ccgggtaacc gaaggcccgc atgatcggca gcccgcggaa atcgacgatc aaattgttat
     2101 agccaaaact ggtgccacgc tcggtcagga ggaggtcctc gctgccggag gcccgcacct
     2161 tctccaccac gttgaccatg tcgcgcgggg ccacgaactg gcccttcttg acgttcaccg
     2221 gccggccgct ccgggcgcag gccacgatga ggtcggtctg ccggcagagg aaggcgggca
     2281 cctgcagcac gtcgagcacc tgggccgcgg gcgcgacctc ggtcacggcg tggacatcgg
     2341 acagcaccgc gaggccggtc tcgtccttga ccttctggag gatgcgaaga ccttcggtca
     2401 gcccgggccc ccggtaggaa tggatcgagg agcggttggc cttgtcgtag gacgacttgt
     2461 agatgaaggg cacgcccgcg tccgcggtca cccgctgcag ccgctccgcc atcatcagcg
     2521 cgtgcttctc gttctcgatg gcgcaggggc cgccgatgag ggcaagcggg gcgccgccgc
     2581 cgatggtgac ggagccgatc cggaccgcgc gtgtcggggt cacgcgcccc cctggtggtc
     2641 gagcgccgcc ttgatgaacg cggcgaagag cggatgcggc tcccacggcc gcgagcggaa
     2701 ctcgggatgg aactgcccgg cgacgaacca cgggtgatcg ggcagctcga tgatctccac
     2761 cagctgcttc tcggcccaga ggccggagat ccgcagcccg ttcttctcga gccgggccag
     2821 atagtcgttg ttcacctcgt accggtgccg gtgacgctcc tcgatcaggc cctgcccgta
     2881 ggcccgcgag gccgcggtgc cctcgctcag cagcaccggg tagagcccga ggcgcatggt
     2941 gccccccttg tcctggacgt ggcgctgctc gggcaggaga tcgatcacgt tgtgggcgac
     3001 ggtgggattg aactcggtgg agttggcgcc ctcgagcccg cacacgtggc gcgcgtactc
     3061 gatgaccgcg cactgcatgc ccaggcagat gccgaagtag ggcacgcgct gctcgcgcgc
     3121 gtaacggatg gcctggatct tcccctcgat gccccggtcc ccgaagccgc cgggcaccag
     3181 gatgccgtcg agcccggcca cgtggccggc gagcccctcg cgctcgatgc gctcggcgtc
     3241 cacccacacc acctcgaccg ctgcctcgtg cgcgatgccc ccgtgggcga gcgcctcgta
     3301 gaggctcttg taggaatcct tcacctcgat gtacttgccc accaccccga tgcggcagcg
     3361 gcggcgcggc gccttgatcc ggcgcacgat ctcctcccag cgggagagat cgatcggccg
     3421 ggagggcaac cccagcagct tgatgatgga ctcgtccagg ccctggtcgt ggaaggcgat
     3481 cggcacctcg tagatcgtgt ccacgtcgcg ggccgcgatg accgcttcct cctcgacatc
     3541 gcagaagagc gcgatcttgg acttgatgga cggggtgagc ggatggtcct tggcggtccg
     3601 gcacaggagc acgtcgggct ggatcccgat ggcccgcagc tccttgaccg agtgctgggt
     3661 cgccttggtc ttgagctcct gcgcggcctg gatgtacggc accagcgtga ggtggacgta
     3721 gatgacgttg tccctgccca cgtccttctt gaactggcgt accgcctcga ggaacggcag
     3781 accctcgatg tcgccgacgg tgcccccgat ctcgatgatc accacgtcga cgccggcggc
     3841 caccttccgg atcgacgcct tgatctggtc ggtgatgtgc gggatgacct ggacggtgcg
     3901 gcccaggtag tccccccggc gctccttctc gatcacggtc ccgtagacct tcccggtggt
     3961 gacgttgttg tcccgggtca tccgggccga ggtgaagcgc tcgtagtggc cgagatcgag
     4021 gtcggtctcg ccgccgtcgt cggtcacgta gacctcgccg tgctggtacg gactcatggt
     4081 ccccgcatcc acgttgatgt aggggtccat cttctgcagc gtgacccgga agccgcgcgc
     4141 ctcgagcaag gacccgatcg atgccgcggc cagccccttg cccagggagg acacgacgcc
     4201 gcccgtcacg aagatgaatc tggtcgtcac ctcacgcctc ctttcccgat gtgagccatt
     4261 ctttcacgag ttcccgctcc tcggccaccg agcccgcccc gccgtccagc cggtgccgtc
     4321 gcaacatgcc gagcgccgcg cccacgcgcg ggccgcccgg caccccgagc gcgagcagat
     4381 cgcggccggt gagccgtggc cgcaccgcgc gcccgtgggc gagataccac tggatccgcc
     4441 ggcgcgcccg cgccccgccc cggagccagg ccgcgagcgc ggccgactcc ggcgccccgt
     4501 cgaggagctc gcccaccacg ctgggcggca gcccccgggt atccaggcgc cgggcgagcg
     4561 gcccggccag cgccccggcc tcgagccgcg cccgcggctc gcccgcgagc gccagccggt
     4621 cgaggctgcg ctcgaccacc tgggcggggc ggccgagcgt gagcgcgagg agcgcgagct
     4681 cggccgggtc cactaccacg cccgccgact tggcccagcg cccgaggcgc tcggcagccg
     4741 cgaggaaccg agaggaagct ccttcgagat tccagagact tgtggccttc catctcacga
     4801 gtcgctcgaa gaccagacgg ggccgcggct cggcccccgc cagctcgatc tcacgccaga
     4861 gacgctggcc cgagagagcg gcatacccgg gccgctccac cgccagccgg atcgccgcca
     4921 gggtggccgc atccggcctg agaccgaggc ggctcgcgta gcgcgcggcc cggaacactc
     4981 gggtggggtc ctcgacgaat gagaggggat ggagcgcccg cagccgccgg cgccggacat
     5041 ccgcctggcc gcccagcggg tcgagcaggc ggccgaagcg atcgggcgac agggccaggg
     5101 ccagcgcatt caccgagaag tcgcgacggc ggaggtcctc cagcagaccc gccggctcca
     5161 cctcgggcag cgcgccggcc tccggatagt gctcgcgtcg agccgaggcc acgtccacgc
     5221 ggccgagcgc caccccgtcg agccccgcgc ccgccggcgc gcggcccccc tcgatggacg
     5281 cggtgccgaa gctccggtgc acgagcacgg tgccgccgat ctcctccgcg agccgccgcg
     5341 cgaaggcgac gccgtccccc tcgacgacga ggtccacgtc gggcggcgcg gcgccggcca
     5401 gcaggtcgcg gacgaagccg cccaccgcga aagccggcgc gcccagcccc tcgccgatct
     5461 tgccggccac ctggagcagc cagatcctgg cgtcgttcag cggcgggggc cccggactgg
     5521 cactcggacg cggcttcgct tcaccagcgc gctcgaacag cgcgaggacc gaggattcgg
     5581 gcggcgtcag gtcgacgcga tcgcggtcca ccgcgccgat cacgcgcgag ccccggcgca
     5641 ccaggacgag gggcgtcccc gcccgcaggc gtcgccggac gtcgatctcg gaggcgcccg
     5701 cggccacgct cggcaggccg cgccaggcca cttcacgcgc gagccgccgg tcgaggcccc
     5761 aggcggcggc ccgcgccagc tccgacacgc gcgccgcgcg ccccggaccg aggaggacca
     5821 ccgtcgcgcc gctctcgcgc gcgcgggcga gcgcccgcgc cacggacacg ccgggcccgc
     5881 aggtcagcac gcgcgggtcc atgagcgccg cgatgcccgg ctccacctgc ggatacacgt
     5941 ggccggcctt cgccatctca gcccgctcgc tcgaggccgc gcgcgcgagc gacccgagcc
     6001 acgcgcgccg cccgcgggtg gaacttccgg tacatgtcgt ggacgacgct cgacggcagg
     6061 tgcgtgtaga tctgggtggt cgagatgtct acgtggccga gcatggcctg gaccgagcgc
     6121 aggtcggcgc cgcgctcgag cagatggctc gcgaaggagt ggcgcagggt gtggggcgag
     6181 accgcggcgc ggaggcccgc gcgctgcgcc gcgcgacgga tgaggcccca gagggcctgc
     6241 cgcgacatcg cgccgcccga gcggttgagg aacagcgcgg ggcactcgcg ccgcacctgg
     6301 cgcgggcgga tcgtggtgag gtaccggcgt acccagtcga gcgcccgtgc gcccgcgggc
     6361 acgaggcgct gccggtcgcc ctttccgatc accatcacgt agcccgcctt gaagttcacg
     6421 tcctcgatcc gcaggccgag agcctcggag gcgcggagcc cggaggcgta gagcaattcg
     6481 agcagcgcgc ggtcccgcag cccgtccggc cggctcgtgt cgggcgcctc gacgagcgcg
     6541 gccgcgtccg ccatggacag ggtgcggggc agccgccggg ccggacgcgg gctgtccagg
     6601 tgctgggtcg gatcgcgcgg cacgcgcccg gtatcgagga ggaagcggta gaggccgcgc
     6661 accgcggaga gatgccgggc gatgctgcgg ctgccgaggc cccgccggcg gaggccaccc
     6721 aggtagcgcg agagatcggt gacacccacg tcaccgatcg cgcggtgctc ccgctgcagg
     6781 aaggtctcga atcccttgag gtcccgacgg tacgcggcga gcgtgttcag cgcggcgccc
     6841 cgctcgatct ggaggccgcc gaggtactcg tcgatcagcg ccgcggcttc ggagacgact
     6901 cccgcggtcc ccctctccgc ggacgccccg ggccgagagc gcatcgatca catccggagt
     6961 gggaggccca aaaagcgacg gccccggtat tggccgatgc gtcgtatcca ggccatgacc
     7021 ggggcgtccc ctgcgaagtc caggagcatg agcgagtcta tcatcggcgc cgggggcggg
     7081 gcaaggatca gcggcgcgcg tgcagcatgc cgagcagcgc gcgcagctcc ggcgcgcgga
     7141 tcgccgccga catggccacg tacaccgcca cgccgccgac cagcgccagc accatccgcg
     7201 cgaggcccag caccaccggc tccggcacca ccgccaggaa tccctccacc cacgcgcacc
     7261 acagcgcgag cgcggcggtg gccacgaccg tgcggcccag cgacccccag atctccgagc
     7321 cgcgcggtcc gcccagccgg cggtggagca gccagcagag cccgatcacg ttgacgtagg
     7381 aggacagcga cgaggcgagc gcgagcccgg tgtggcgcag cggccacatc aggaccaggg
     7441 ccagcgccac gttcaccgcg accgacacga acccgacgat caccggtgtc cgcgtgtcgc
     7501 cgagcgcgta gaaggtctgg gccgcgatgc gggtggccga gaacgcgggg agccccaccg
     7561 cgtagccggc cagcgcctgg gcggtgagcg aggcgtcgcc ggcggtgaac tctccccgct
     7621 ggaagagcac ctggatgatg ggcccgccca ggagcaggag ccctaccgtc gcgggcaccg
     7681 ccacgaaggc ggagagccgc agcgcgaact cgagcgtggc ccgcagcgcg gggaactccc
     7741 ggcgcgcggc ctgggccgac atgctgggca gcgtggcggt ggcgagcgcg atgccgaaca
     7801 cgcccagcgg gaattccatc acccggtccg cgtagtacag gtacgacacg gtgcccgtgg
     7861 gcagcagcga ggccagcagc gtgttgacca ccaccgtgat ctgcaccgcg gccagcgcga
     7921 acaccgccgg ccagaggcgc cgcgcgatgc gcgccaccgc gggatgacgc cactcgagcg
     7981 aggggcggag cggcaccccg atccgccgca gctcggggag ctggacgaga agctggccga
     8041 gcccccccac cagcacgccg atcgccagcg acatgatcgg ggtggtgagg cgcgaggcga
     8101 ggcccaccac cgccgcgatc atcgcgaggt tgaggacggc gggccc
//
LOCUS       DASUFA010000170         6603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_40459, whole genome shotgun sequence.
ACCESSION   DASUFA010000170 DASUFA010000000
VERSION     DASUFA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40459"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VFX87_06060"
     CDS             complement(<1..1306)
                     /locus_tag="VFX87_06060"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4994511.1"
                     /translation="MDAAGSVVDSLVIREGRVAFAGRRRDINPAPQEPTLDLGGRAVL
                     PGLVDGHGHLMLLARARLELNLATAGSEDEIARMVAAAAARLGSGEWITGRGWDQTRW
                     PGQRFPTRASLDRAAPGHPVALIRVDGHATWASGEALTRAGITRHSTDPQGGLIPKDR
                     NGEPTGILIDLAQDIIRGLIPAPSEERFDQAVRDTVAECLAKGLTGAHEPGLDLSAVA
                     SYTRLIERGQFPFRVFAALSGKKAWGRYRDGAPETVGDGRLVVGAVKLWLDGALGSRG
                     AALHSPYCDDPGNTGLVLVPPEDVERIAREVSARGFHVWVHAIGDRANTMVLDVFERV
                     RAVGGPQSRLRVEHAQILTPADIPRFARLGVVPSMQPTHCTSDMRWAGERLGPERLAG
                     AYAWRSVLDTGAYIAGGSDFPVEDANPFHGLHAAVTRRPREGD"
     gene            complement(1360..2211)
                     /locus_tag="VFX87_06065"
     CDS             complement(1360..2211)
                     /locus_tag="VFX87_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4994512.1"
                     /translation="MSWIDPNFPHSATPTDLVGRVLGLNPGMMTGPGTNTYLVGRSYP
                     ILIDTGAGESDYPALFQRYLRARGWRRPERIVLTHRHRDHLGGVGQLRELFPGVPVSK
                     LIFKDAGLPEDVDMLRDGELVEGDGVTLVPIHTPGHASDHLCFFLKEEQALFSGDLIL
                     NGSTTVIPDEDGDLGQYLDSLRRVQALGVRHIYPAHGPVIEDGPGKIQEFIDHRMLRE
                     RQILEVLGGGPRTVPEIVKVIYADVMPALHRMAGMSVHSHLRKLKNEARVSEETVGGG
                     PSRWTLL"
     gene            2314..3309
                     /locus_tag="VFX87_06070"
     CDS             2314..3309
                     /locus_tag="VFX87_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX4994513.1"
                     /translation="MVLRAALVLLTVLGLVAPAPAQVVTLKLATIVGNDNPFTTSAYK
                     FKEIAEAKSGGRIKVEVYPAGQLTKNEIVAIEGMQLGTVDIAAVSLPSAGGKFDPKLL
                     APDVPFLYQSREHVWRVMDGPVGKELMKHFEPLGAKGFCYGGGFGFRGVLSNKRPVFV
                     PDDLKGQTIRVPPIPTLVEIFKQFGANPVPMIWGEVYMAVRQGTVDGLELPVTTAVSD
                     KFHEITKYYSKTQHSYPAAAWLMAKARYEGLPADLKKVIDETMTASCAQHRQDELKAE
                     VEGLAAMKAKGMQVNEVKDLRLFQERAQPVWKFLEQKAGKELVDRVIAEARATAK"
     gene            3311..3748
                     /locus_tag="VFX87_06075"
     CDS             3311..3748
                     /locus_tag="VFX87_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX4994514.1"
                     /translation="MLVDWVCLGLMLVLVVDVFLGVWSRYVLQATFQWYDEVARLCFV
                     WMVFLGAASAVRRGAHFRLHLLIDRLGPRLRRANDLVVGLLVVVFGGVLVAGGVAMWP
                     VARGQVSDSLELSMVWFYGALPVGGALMILFSLPHLWRLARGR"
     gene            3745..5022
                     /locus_tag="VFX87_06080"
     CDS             3745..5022
                     /locus_tag="VFX87_06080"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX4994515.1"
                     /translation="MILVCLVFLVLLAIGVPVAVTVGLAGYIGAILAAPVPLAVTVQT
                     ILHQVDSFVLLSLPLFILAGALMETGGIAHRLVRLAVALVGWVRGGLGMAVVAAEYIF
                     SGISGSTVADVSAVASTTIPGMVRAGYSKELAVAVVSAASAMGILVPPCILMIVIGSV
                     ASLSVAALFTAGFLPAVVMALVLMAYIWRTAGRMGIAAEAKPTLGELWVAFRDALIPL
                     GMPAIIFGGILGGIMTPTEASVLAVLYAAVVGLFVYREIKWKDLPAIVMNSAMITGAV
                     GLLLGTAGVVSWFLTVQQMPTALIRIMTVVPGSSLVFLFVSAVIFIFFGAVIEGLPAV
                     VILLPSLLPVAAGLGIDPIHYAIVIVAAVGIGLFLPPIGVGLFIACGIAEVSVDRATR
                     AMLPFVAVLLIGLLIIILVPQITLILPRLFKLL"
     gene            complement(5035..5724)
                     /locus_tag="VFX87_06085"
     CDS             complement(5035..5724)
                     /locus_tag="VFX87_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994516.1"
                     /translation="MLEVHGIHTYYDESHVLHGVSLRVAAGEAVALLGRNGAGKTTAI
                     RTIVGFTPPRAGRVLFEGQAIERWPAYRIARRGLALVPQGRRIFAPLSVRENLLLGAR
                     SEGWTLERVFELFPRLRERQAQLGGTLSGGEQQMLAIGRALLTNGRLLLLDEPSEGLA
                     PIIVREIGTILQVLKAQRLSLLLVEQNYHLALRVADRVYVMNKGQIVYEGTPAGLEAD
                     EEVKRRYLGVA"
     gene            complement(5728..6474)
                     /locus_tag="VFX87_06090"
     CDS             complement(5728..6474)
                     /locus_tag="VFX87_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994517.1"
                     /translation="MTAPVLATQAVTRAFGALVALNRVSFEVAPRERRAIIGPNGAGK
                     TTLFNVITGQLAPTSGRILLDGAPIGGLAPHAVARRGISRSFQRNNLFPKLAVEENLR
                     LAAAVGAPGTWDLLGSVTRRRGPLDRAREVARVVGLSDRLGEPAGRLSYGEQRQLEVG
                     VALATSPRLLLLDEPTAGMSPEETLRMTRMLEALPRDVTLLIIEHDMDVVGSLADRVT
                     VLHYGEVLTEGTFAQVKADPRVYEVYLGSA"
BASE COUNT          900 a         2320 c         2373 g         1010 t
ORIGIN      
        1 cgtcgccctc gcggggccgc cgcgtcaccg ccgcgtgcag gccgtgaaac ggattggcgt
       61 cctccaccgg gaagtcggag ccgcccgcga tgtacgcgcc ggtgtcgagc accgatcgcc
      121 aggcgtaggc gcccgcgagc cgctcggggc cgaggcgctc gccggcccag cgcatgtcgg
      181 aggtgcagtg ggtgggctgc atgctcggca ccacgcccag gcgcgcgaag cgcgggatgt
      241 cggcgggcgt caggatctgc gcgtgctcga cgcgcaatcg tgattgcggg ccgcccacgg
      301 cccgcacgcg ctcgaagacg tcgagcacca tggtgttggc gcgatcgccg atggcgtgca
      361 cccacacgtg gaagccgcgg gcgctcacct cgcgggcgat ccgctccacg tcctcgggcg
      421 gcaccagcac gagcccggtg ttcccggggt cgtcgcagta gggcgagtgc agcgcggccc
      481 cgcgcgagcc gagcgcgccg tcgagccaga gcttcaccgc gcccaccacc agccggccgt
      541 cgcccacggt ctcgggcgcg ccgtcccggt agcgacccca ggccttcttg ccggagagcg
      601 ccgcgaagac gcgaaacggg aactggccgc gctcgatcag ccgcgtgtac gacgcgaccg
      661 cggagagatc gaggccgggc tcgtgcgcgc cggtgagccc cttggcgagg cactccgcga
      721 cggtgtcgcg caccgcctgg tcgaagcgct cctccgaggg cgccgggatc agcccgcgga
      781 tgatgtcctg ggccaggtcg atcagaatgc cggtgggctc accgttgcgg tccttcggga
      841 tcaggccgcc ctgcggatcg gtggagtggc gcgtgatgcc ggcgcgggtc agcgcttcgc
      901 cgctggccca cgtggcgtga ccgtccacgc ggatgagcgc caccggatgg ccgggcgcag
      961 cgcgatcgag cgaggcgcgg gtcggaaacc gctgcccggg ccagcgcgtc tgatcccagc
     1021 cgcgcccggt gatccactcc cccgacccta gccgcgccgc ggccgccgcg accatccgcg
     1081 cgatctcgtc ctccgagccc gcggtggcca ggttcagctc gagccgcgcg cgggccagga
     1141 gcatcaggtg cccgtggccg tccaccagcc ccggcaacac cgcccgcccg ccgaggtcga
     1201 gcgtgggctc ctgcggcgcg ggattgatgt cgcggcgccg gcccgcgaag gccacgcggc
     1261 cctcgcggat cacgagggaa tccacgacgg agccggccgc gtccatcgtg tggatccgac
     1321 cgttggtcag cagcacggga tcggcgcgcg cggtcggagc tagagcaggg tccagcggga
     1381 gggaccgccg cccaccgtct cctcgctcac ccgcgcctcg ttcttgagct tgcggaggtg
     1441 cgagtgcacg gacatgccgg ccatgcgatg cagcgcgggc atcacgtcgg cgtagatcac
     1501 cttgacgatc tcgggcacgg tgcgcggacc gccgcccagc acttccagga tctggcgctc
     1561 gcgcagcatc cggtgatcga tgaactcctg gatcttgccg gggccgtcct cgatcaccgg
     1621 gccgtgcgcc ggatagatgt gccgcacgcc gagcgcctgc acgcggcgga gcgagtcgag
     1681 gtactgcccg aggtcgccgt cctcgtcggg gatcacggtg gtggagccgt tcaggatcag
     1741 gtcgccgctg aagagcgcct gctcctcttt caggaagaag cacaggtggt cggaggcgtg
     1801 gccgggggtg tggatcggca cgagggtcac gccgtcgccc tccaccagct cgccgtcccg
     1861 cagcatgtcc acgtcctcgg gcaatccggc gtccttgaag atcagcttgg acaccggcac
     1921 gcccgggaag agctcgcgga gctggccgac accgcccagg tggtcgcgat gacggtgggt
     1981 caggacgatg cgctccggcc gccgccagcc acgcgcgcgc aggtagcgct ggaacagcgc
     2041 gggatagtcg gactcaccgg ccccggtgtc gatcaggatc ggataggagc ggccgaccag
     2101 gtaggtgttg gtgccgggcc ccgtcatcat gccgggattg agcccgagca cgcggccgac
     2161 caggtccgtg ggcgtcgcgc tgtgcggaaa gttcggatcg atccagctca tggggccgag
     2221 tgtagcaaaa gtagcggcgc ttgctacgcg ggggagcctg ggatataagc gagcccaaac
     2281 gccgcccacc cccaacgccg ctggaggctc gtcatggtcc tacgcgcagc gctcgtcctg
     2341 ctcaccgtcc tcgggctcgt cgcacccgcg cccgcccagg tggtcaccct caagctcgcc
     2401 accatcgtcg gcaacgacaa ccccttcacc acctccgcct acaagttcaa ggagatcgcg
     2461 gaggccaaga gcggcggccg catcaaggtg gaggtctacc cggccgggca gcttaccaag
     2521 aacgagatcg tggccatcga gggcatgcag ctcggcaccg tggacatcgc ggcggtctcg
     2581 ctgccgtccg cgggcggtaa gttcgatccc aagctgctcg cccccgacgt cccgttcctc
     2641 taccagagcc gcgagcacgt gtggcgcgtg atggacggcc cggtgggcaa ggagctgatg
     2701 aagcacttcg agccgctcgg ggccaagggt ttctgctacg gcggcggctt cggcttccgc
     2761 ggcgtgctct ccaacaagcg gccggtgttc gtgcccgacg atctcaaggg ccagaccatc
     2821 cgcgtcccac cgatccccac cctggtcgag atcttcaagc agttcggggc caacccggtg
     2881 ccgatgatct ggggcgaggt gtacatggcg gtgcgccagg gcacggtcga cgggctcgag
     2941 ctcccggtga ccaccgcggt ctccgacaag ttccacgaga tcaccaagta ctactcgaag
     3001 acgcagcact cgtatcccgc cgccgcctgg ctcatggcca aggcgaggta cgagggtcta
     3061 ccagcggacc tcaagaaggt gatcgacgag acgatgaccg cctcgtgcgc ccagcaccgc
     3121 caggacgagc tgaaggcgga ggtggagggc ctcgcggcca tgaaggccaa gggcatgcag
     3181 gtcaacgagg tgaaggacct ccgcctcttc caggagcgcg ctcagccggt ctggaagttc
     3241 ctcgagcaga aggcgggcaa ggagctggtg gatcgggtga tcgcggaagc ccgggccacc
     3301 gcgaagtagc gtgctcgtcg actgggtctg cctggggctg atgctggtcc tggtggtgga
     3361 cgtcttcctc ggcgtgtgga gccgctacgt gctgcaggcg accttccagt ggtacgacga
     3421 ggtcgcgcgg ctctgcttcg tctggatggt gttcctgggc gccgcgtccg cggtccggcg
     3481 cggcgcccac ttccgcctcc atctcctgat cgaccgcctc ggcccccgcc tgcggcgcgc
     3541 gaacgatctg gtggtgggcc tgctcgtcgt ggtcttcggc ggcgtgctgg tggcgggcgg
     3601 cgtggccatg tggccggtgg cgcgcgggca ggtctccgac tcgctcgagc tgtcgatggt
     3661 gtggttctac ggtgcgctcc ccgtgggggg cgcgctcatg atcctgttct cgctcccgca
     3721 tctctggcgc ctggcccgcg gccgatgatc ctcgtctgcc tcgtcttcct ggtactgctg
     3781 gccatcggcg tgccggtggc ggtgaccgtg gggctggccg gctacatcgg cgccatcctc
     3841 gcggcgccgg tcccgctcgc ggtgacggtg cagaccatcc tccatcaggt ggactccttc
     3901 gtcctgctct cgctgccgct cttcatcctg gccggcgcgc tcatggagac gggcggcatc
     3961 gcgcatcggc tcgtgcgcct ggcggtggcg ctggtcggtt gggtgcgcgg cggcctcggc
     4021 atggcggtgg tggcggccga gtacatcttc tcgggcatct cgggctcgac ggtggccgac
     4081 gtctccgcgg tggcgtccac caccatcccg ggcatggtgc gcgccggcta cagcaaggag
     4141 ctggcggtgg cggtggtgtc ggcggcctcc gcgatgggca tcctggtgcc gccctgcatc
     4201 ctgatgatcg tgatcggctc ggtggcgagt ctctcggtgg ccgcgctctt caccgccggc
     4261 ttcctgcccg cggtggtcat ggcgctggtg ctgatggcct acatctggcg caccgcgggc
     4321 cgcatgggca tcgcggccga agccaagccc accctcggcg agctgtgggt ggccttccgc
     4381 gacgcgctga tcccgctcgg catgcccgcg atcatcttcg gcggcatcct gggcgggatc
     4441 atgacgccca ccgaggcctc ggtgctggcc gtgctctacg cggcggtggt gggcctcttc
     4501 gtctaccgcg agatcaagtg gaaggacctg cccgccatcg tcatgaactc ggcgatgatt
     4561 accggcgcgg tgggcctgct gctcggcacc gccggggtcg tctcgtggtt cctcaccgtt
     4621 cagcagatgc cgaccgcact gatccggatc atgacggtgg tgcccggcag cagcctggtc
     4681 ttcctcttcg tcagcgccgt catcttcatc ttcttcggcg cggtgatcga gggcctcccc
     4741 gcggtggtga tcctgctgcc gagcctcctg ccggtggcgg ccgggctcgg catcgacccc
     4801 atccactacg cgatcgtcat cgtggccgcg gtgggcatcg ggctcttcct gccgccgatc
     4861 ggggtgggcc tcttcatcgc ctgcgggatc gcggaggtct cggtggatcg agccacgcgc
     4921 gcgatgctgc ccttcgtggc cgtgctgctg atcgggctcc tcatcatcat cctggtgccg
     4981 cagatcacgt tgatcctgcc acgcctcttc aagctgctct gacgcggtgc gcgctcaggc
     5041 cacgccgagg tagcgccgct tgacctcctc gtcggcctcc agcccggcgg gcgtcccctc
     5101 gtagacgatc tgccccttgt tcatcacgta gacccggtcg gctacccgca gggccaggtg
     5161 gtagttctgc tccaccagca ggagggagag gcgctgggcc ttcagcacct gcaggatggt
     5221 cccgatctcg cgcacgatga tcggcgccag gccctccgac ggctcgtcga gcagcagcag
     5281 gcggccgttg gtgagcagcg cccggccgat ggcgagcatc tgctgctcgc cgcccgagag
     5341 cgtgcccccg agctgggcct ggcgctcgcg caggcgcggg aacagctcga acacgcgctc
     5401 gagcgtccag ccctcggagc gcgcgcccag cagcaggttc tcgcgcaccg agagcggcgc
     5461 gaagatgcgg cgaccctgcg gcaccagggc caggccccgg cgcgcgatac ggtaggcggg
     5521 ccagcgctcg atcgcctgcc cctcgaagag cacgcggccg gcgcgcggcg gcgtgaaacc
     5581 cacgatggtg cggatcgcgg tggtcttgcc cgcgccgttc cgccccagca gcgccaccgc
     5641 ctcgcccgcg gccacgcgca gcgaaacgcc gtggagcacg tggctctcgt cgtagtaggt
     5701 gtggatgccg tgcacctcga gcaccgctca ggcgctcccc aggtagactt cgtagacgcg
     5761 cggatcggcc ttcacctggg cgaaggtgcc ctccgtgagg acctcgccgt agtgcagcac
     5821 cgtgacgcga tcggcgagcg agcccaccac gtccatgtcg tgctcgatga tcaggagtgt
     5881 gacgtcgcgc ggcagcgcct cgagcatgcg cgtcatgcgc agcgtctcct cgggcgacat
     5941 gccggcggtg ggctcgtcca ggagcaggag ccgcggcgag gtggcgagcg ccacccccac
     6001 ctcgagctgg cgctgctcgc cgtaggacag gcgcccggcg ggctcgccca gtcggtcgga
     6061 caggcccacg acgcgggcga cctcgcgggc ccggtcgagc ggcccgcggc gtcgcgtgac
     6121 gctgcccagc agatcccagg tgccgggcgc gcccaccgcg gcggccagcc gcaggttctc
     6181 ctccaccgcg agcttcggga agaggttgtt gcgctggaac gagcgcgaga tgccgcggcg
     6241 ggccaccgcg tgcggagcga gcccgccgat cggcgcgccg tcgaggagga tgcggcccga
     6301 cgtcggggcg agctggccgg tgatgacgtt gaagagcgtg gtcttgcccg cgccgttggg
     6361 gccgatgatg gcccggcgct cgcgcggggc gacctcgaac gacacgcggt tgagcgcaac
     6421 gagcgcgccg aaggcgcgcg tcaccgcctg ggtcgccagg accggcgcgg tcatgcgcgc
     6481 aggcccaccc ggccgcgcag cgcgcccacg atgccgccgg gggcgaagag cacgaagagg
     6541 atgaaggtcg cgcccaggat cagcatccag cgctcggtgt aggtgctgac caggctctgc
     6601 agc
//
LOCUS       DASUFA010000171         5285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1071005, whole genome shotgun sequence.
ACCESSION   DASUFA010000171 DASUFA010000000
VERSION     DASUFA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071005"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..238)
                     /locus_tag="VFX87_06095"
     CDS             complement(29..238)
                     /locus_tag="VFX87_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX4994518.1"
                     /translation="MKIILRNPRKEIEVDGKRRVKELLVELDILPSTVLVIRGNELLT
                     GDTVVGGDDVLEVRPVMSGGSTGTP"
     gene            complement(235..774)
                     /locus_tag="VFX87_06100"
     CDS             complement(235..774)
                     /locus_tag="VFX87_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4994519.1"
                     /translation="MAWPVPVEALWNDLQRVRSELLKETEGLSQAQVDWRPAEQDWSV
                     GEILHHLSLAEVGTGKLTSKLFKDAPQPLPPFPLDLRSFAPVPPPPPGPAKAPEVVYP
                     ERGHALERLVADLKAIRERSRQSIERLAAVDPRGLKWRHFTLGEMDLAQWWLLHVRHE
                     ADHLEQLRAVKAAQGFPRP"
     gene            810..1394
                     /locus_tag="VFX87_06105"
     CDS             810..1394
                     /locus_tag="VFX87_06105"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4994520.1"
                     /translation="MSEPTGTPAAGSAWSRTPWRRTSTRPVYDNQWISVREDVVELPD
                     GRSTLYGVVQCSECVGVLPFLDRDTVLLVGQYRYVAGDFFWEIPTGGQHAGETPTEAA
                     QRELAEEAGHEAGRLVELGDFHTSKSILREVAHLYVAQDLRPASRPPDPTEFIERRTF
                     PFAEVLAMVERGEVKDAMTIIAVLRAARLREARG"
     gene            complement(1426..2199)
                     /locus_tag="VFX87_06110"
     CDS             complement(1426..2199)
                     /locus_tag="VFX87_06110"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX4994521.1"
                     /translation="MNRDEIGELAAAYALGSLEAEDRARFEALLRTGDPEATAALRDF
                     EGALTDLAAAAPETPPPAVKAALMERIAAAPPAAPAPVRPLPRRRRAIWPVVLSGAMA
                     AGLAAIAVGWSVSSTYEKRLDALARDAEQLKAELRSQQTVITILRDPATQMVALAGQA
                     PAPTARARMMWHAKAGGVFVAAGLPAAPAGKAYQLWAIAGANAPVSAGVFSVDASGTG
                     SLSVRPLPGVATVNAFAVTLEPAGGLPAPSGELYLLGKS"
     gene            complement(2196..2774)
                     /locus_tag="VFX87_06115"
     CDS             complement(2196..2774)
                     /locus_tag="VFX87_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4994522.1"
                     /translation="MSSAGAGSETSALIARIAAGDRDAFSRFYDLLAPTAFGLIRRVL
                     RDPEAAADVLQEVFWQVWREAPQYDPTRGSPEAWLVMRAKTRAIDRLRSMRRRDRTFV
                     APVDESVAQRAQEPAENPAVVAEDRGLVQTALTQLPEPQRRVIELAFFDGLTQSEIAT
                     RLGEPLGTVKTRARLGLERLRGALRGERVSAT"
     gene            complement(2783..4072)
                     /locus_tag="VFX87_06120"
     CDS             complement(2783..4072)
                     /locus_tag="VFX87_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX4994523.1"
                     /translation="MATFLDRHLETLGSDALLTYQWARLERLAGAAVPANAFVTARWK
                     RAGLATPQDLRGWEDFHRLPLISKSELMEDQAAHPPFGSNLSYPLDRYVRVHQTSGTT
                     GTPLRWLDTQESWEWWARCWTFVLRAAGLGPGDRVFFPFSFGLFVGFWAGFEGARALG
                     ALAIPGGGQDSAQRLAAMEALGATAVCCTPSYALHLAEVARERGVALDRLGVRATVHA
                     GEPGAGIPAVRARIEEAWGARAYDHAGMTEMGAYGFECAAQSGLHVNEAEFIAEVIDP
                     ATGGLADEGELVLTNLGRLGSPLLRYRTGDRVRVARTPCECGRTFLRLEGGILGRVDD
                     MLVVRGVNVFPSALEGIVRRFPDVDEFVIEVYRRGQLDEVRLLLEVKGHAQAVVAAVQ
                     EAVRVDLGIRVEAVPVPGQSLPRYELKARRLVRRASE"
     gene            4488..>5285
                     /locus_tag="VFX87_06125"
     CDS             4488..>5285
                     /locus_tag="VFX87_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4994524.1"
                     /translation="MTVLFADLKGSTELLADRDPEEARKLLDPVLDRMMEAVHYYEGT
                     VNQVMGDGIMALFGAPLAHEDHAVRGCYAALRMQESVKRYAEEVSKSAGVPIHIRVGL
                     NSGEVVVRSIGNDLHMDYSAVGQTTHLAARMEQTALPGTILIPETMLSLTRDAVAVSS
                     LGPRSVKGLAAPIEVYEVTGASTTGSRRQAATARDVTRFVGRHAEMATLQQALERAAA
                     GHGQVVAIAAEPGVGKSRLIREFLHADAAHGWRILEAIGVATGKTTAY"
BASE COUNT          762 a         1950 c         1874 g          699 t
ORIGIN      
        1 gcggccgccc cgccgcactt gcggcacttc acggcgtccc cgtgctgccg cccgacatga
       61 ccgggcgcac ctcgagcacg tcgtcgccac ccaccacggt atccccggtc aggagctcgt
      121 tgccgcggat gaccaggacg gtgctgggca ggatgtcgag ctcgacgagg agctccttga
      181 cccggcgctt gccatccacc tcgatttctt tccggggatt gcgcaggatg atcttcacgg
      241 gcgcgggaag ccctgggcgg ccttcacggc gcgaagctgc tcgaggtgat cggcctcgtg
      301 ccgcacgtgc aggagccacc actgcgccag atccatctca cccagcgtga agtgccgcca
      361 cttgagcccg cgcggatcca ccgcggccag gcgctcgatc gactggcggc tccgctcccg
      421 gatcgccttc agatcggcga ccagccgctc cagcgcatgg ccccgctccg ggtacaccac
      481 ctccggcgcc ttggcgggac cgggaggcgg cggcgggacg ggcgcgaagc tcctgaggtc
      541 gaggggaaac ggcggcaggg gctgcggcgc gtccttgaag agcttggagg tcaacttgcc
      601 ggtgcccacc tcggccaggc tcaggtgatg gaggatctcg ccgaccgacc agtcctgctc
      661 ggccgggcgc cagtccacct gggcctggct gaggccttcg gtctccttca ggagctcgga
      721 gcggaccctc tgcagatcgt tccatagcgc ttcgacgggg accggccagg ccatcacgtt
      781 cctcccggcc cacatgatac aaccggatca tgagcgagcc cactggtact cccgcggccg
      841 gttccgcatg gtcccgcacg ccctggcgcc ggacctccac ccggccggtc tacgacaacc
      901 agtggatctc cgtccgcgag gacgtggtcg agctgccgga cggccgcagc acgctctacg
      961 gggtggtgca atgcagcgag tgcgtcgggg tcctcccctt cctcgaccgg gacaccgtgc
     1021 tcctggtggg gcagtaccgc tacgtggcgg gcgatttctt ctgggagata ccgacgggcg
     1081 gtcagcacgc gggcgagaca ccgaccgagg ccgcgcagcg cgagctggcc gaggaggccg
     1141 ggcacgaggc cgggcgcctc gtcgagctgg gcgacttcca cacctccaag agcatcctgc
     1201 gcgaggtcgc ccacctctac gtggcccagg acctgcgccc ggcctcgcgc ccgcccgacc
     1261 ccaccgagtt catcgaacgg cgcacgttcc ccttcgccga ggtgctcgcc atggtggagc
     1321 gcggggaggt caaggacgcg atgaccatca tcgcggtgct gcgcgcggcg cggctgcggg
     1381 aggcccgcgg ctaggccgcc ctcaggcctg cgggtcgatc cgacgtcagg acttgcccag
     1441 caggtacagt tccccgctcg gcgcgggcag cccacccgcg ggctcgagcg tcaccgcgaa
     1501 cgcgttgacc gtcgccaccc cgggcagcgg ccgcaccgag aggctgcccg tccccgaggc
     1561 gtccacgctg aacacgcccg ccgagaccgg cgcgttggcg cccgcgatgg cccagagctg
     1621 gtaggccttg cccgcaggcg ccgcgggcag cccggccgcg acgaagaccc cgccggcctt
     1681 cgcgtgccac atcatgcggg cgcgcgcggt cggcgccggc gcctggccgg cgagcgcgac
     1741 catctgggtg gccgggtcgc gcaggatcgt gatcaccgtc tgctggctcc gcagctccgc
     1801 cttgagctgc tcggcgtcgc gcgccagggc gtcgagccgc ttctcgtagg tggacgagac
     1861 cgaccagccg accgcgatcg cggccagccc ggcggccatg gctccgctca gcaccaccgg
     1921 ccagatcgcg cggcgccgac gcggcagcgg gcgcaccggc gccggcgccg ccggcggggc
     1981 cgccgcgatg cgctccatga gcgcggcctt caccgcaggc gggggcgtct cgggcgccgc
     2041 ggcggccaga tccgtcaggg cgccctcgaa gtcgcggagc gcggccgttg cctccgggtc
     2101 gcccgtgcgc agcagcgctt cgaagcgcgc ccggtcctcg gcctccaggc taccgagggc
     2161 gtacgccgct gccagctcgc cgatctcgtc ccggttcatg tcgccgacac cctctctcct
     2221 ctcagggccc cgcgtagccg ctcgaggccg aggcgcgcgc gcgtcttcac ggtgccgagc
     2281 ggctcgccca gccgggtcgc gatctccgac tgcgtcaggc cgtcgaagaa cgccagctcg
     2341 atgacccggc gctgcggctc cgggagctgg gtcagcgccg tctgcaccag gccccggtcc
     2401 tccgccacca ccgccgggtt ctcggcgggc tcctgggcgc gctgggcgac ggactcgtcc
     2461 accggcgcca cgaaggtcct gtctcttcta cgcatggacc ggagccgatc gattgctcgg
     2521 gtcttggccc gcatgacgag ccacgcctcc gggctgccgc gcgtgggatc gtactggggg
     2581 gcctcgcgcc acacctgcca gaacacctcc tgcaggacgt cggcggcggc ctcggggtcg
     2641 cgcagcacgc ggcggatcaa accgaacgcg gtgggcgcga ggagatcgta gaagcggctg
     2701 aaggcgtcgc gatcacccgc ggcgatgcgc gcgatgagcg ccgaggtctc ggaaccggct
     2761 cccgcggacg acatggccac tactactcgc tcgcgcgacg caccaggcgc cgggccttga
     2821 gctcgtagcg cggcaggctc tgcccgggga cgggcaccgc ctcgacccgg atgccgaggt
     2881 ccacccgcac cgcctcctgc accgccgcga cgaccgcctg cgcatggccc ttgacctcga
     2941 ggagcaggcg cacctcgtcg agctgaccgc gccggtagac ctcgatcacg aactcgtcga
     3001 catccgggaa gcgccggacg atgccttcga gcgcggaggg gaagacgttg acgccgcgga
     3061 cgacgagcat gtcgtccacc cgcccgagga tgccgccctc gaggcggagg aaggtccggc
     3121 cgcactcaca cggcgtgcgg gcaacgcgca cccgatcgcc cgtccggtag cggagcagcg
     3181 gcgagccgag gcggcccagg ttcgtgagca cgagctcgcc ctcgtccgcg aggccgccgg
     3241 tggcggggtc gatcacctcg gcgatgaact ccgcctcgtt cacgtgcagg ccggattggg
     3301 ccgcgcactc gaagccgtag gcgcccatct cggtcatgcc cgcgtggtcg taggcgcggg
     3361 cgccccaggc ctcctcgatg cgcgcccgca ccgcgggaat gcccgcgccc ggctcgcccg
     3421 cgtgcacggt ggcgcggacg ccgagccggt cgagcgcgac gccgcgctcg cgcgcgacct
     3481 cggccaggtg cagggcgtag gacggcgtgc agcagaccgc ggtggcgccc agcgcctcca
     3541 tggcggcgag ccgctgcgcg gaatcctggc cgccgcccgg gatcgcgagc gcgccgaggg
     3601 cgcgggcgcc ctcgaaaccc gcccagaacc cgacgaagag cccgaacgag aacgggaaga
     3661 acacgcggtc acccgggccc aggccggccg cgcgcagcac gaaggtccag cagcgcgccc
     3721 accactccca cgattcctgc gtatcgagcc agcgcagcgg cgtaccggtg gtgccggacg
     3781 tctggtgcac gcgcacgtag cggtcgagcg ggtagctgag attgctgccg aagggcggat
     3841 gcgccgcctg gtcctccatc aactcgctct tgctgatgag cggcagccgg tggaagtcct
     3901 cccagccccg taggtcctgc ggggtcgcga ggccggcgcg cttccagcgg gcggtgacaa
     3961 aggcgttggc cggcacggcc gcccccgcca gcctctcgag gcgggcccac tggtacgtca
     4021 ggagcgcgtc cgagcccaga gtctcgaggt gccggtccag gaatgtggcc atgctcgcag
     4081 gatcgaatgc tggcacgaag cgcaactatt gcgcaagccg ccggccggca acctcgctct
     4141 tcttggcggg ctttccttga cgagccggtc gtcaggggct acgctcaacc atcgcttcgc
     4201 ggtcgtgccc cgccagcgac tggaggccgt ggatgctgtg tgcgaagtgc cagcaggaaa
     4261 atccggtcgg cgccaggttc tgtggggact gtggcgatcg tttttccccg acgtgtccca
     4321 gctgtgaagc accccaccga cccgatcaga acttttgctg ggagtgcggc acgcgactcg
     4381 ccgccacgtc cgccgccacg accggcaccc cgggcagtca tatcccccga cacctcgccg
     4441 agcggatcct gacctcgaag gccgcgctcg aaggcgagcg caagcaggtg acggtcctct
     4501 tcgcagatct caagggatcc acggagctcc tcgccgaccg cgatccggag gaagcccgca
     4561 agctcctgga tccggtgctc gaccggatga tggaagccgt gcactactac gagggcacgg
     4621 tcaaccaggt gatgggcgac gggatcatgg cgctcttcgg cgcgccgctg gcgcacgagg
     4681 accacgcggt gcgcggctgc tacgcggccc tgcggatgca ggagtcggtg aagcggtacg
     4741 cggaggaggt ctccaagtcc gccggcgtgc cgatccacat ccgcgtcggg ctcaactccg
     4801 gcgaagtggt cgtgcggtcc atcgggaacg acctgcacat ggactactcg gcggtgggcc
     4861 agacgaccca cctcgcggcc cggatggagc agacggccct gcctggcacg atcctcatcc
     4921 ccgagaccat gctgagcctc acccgcgacg ccgtcgcagt gagctccctc ggcccgcgct
     4981 cagtgaaggg cctggcggcg ccgatcgagg tgtacgaggt gaccggggcg agcacgacgg
     5041 gctcgcgccg gcaggccgcg accgcgcgag acgtcacgcg cttcgtcggg cggcacgccg
     5101 agatggcgac gctgcagcag gccctcgagc gcgccgccgc gggacacggg caggtcgtgg
     5161 cgatcgcagc cgagccgggg gtgggcaagt cgcggctgat ccgcgagttc ctgcatgcgg
     5221 acgccgcgca cgggtggcgc atcctggagg ccatcggcgt cgccaccggc aagacgacgg
     5281 cgtac
//
LOCUS       DASUFA010000172         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1529309, whole genome shotgun sequence.
ACCESSION   DASUFA010000172 DASUFA010000000
VERSION     DASUFA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529309"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFX87_06130"
     CDS             complement(<1..538)
                     /locus_tag="VFX87_06130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX4994525.1"
                     /translation="MTEPFVTLPEIHRAAKARLPRNSYNFGAGGTETETTLRRNRRAI
                     RRLAVRQDILVDVRHIDLSTSLIGIPLSWPVVIAPMGGLVLFHPEGDREMARGAAKGD
                     TLQFLSGATGWSVEAVAEAAPGPKMFQLYHMGDRAWVADLLARVEASGYLSVCLTVDV
                     QVYSRRERDIVARFSPREA"
     gene            554..1108
                     /locus_tag="VFX87_06135"
     CDS             554..1108
                     /locus_tag="VFX87_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994526.1"
                     /translation="MPLKPVPYRSLRALLRRELIGEEHEATAALIRRLAHVKRMGRFT
                     RAEFLTMCRWKSPRSRRHYERNTAGTIRRVSSAVLATRSERERMAQLIALPGVSVATA
                     SAILTLIDPRRYGVLDIRCWQLLFSIRSVAGNERGRAFTVSQWEHYLERLRGHARALG
                     VSARMIEYTLFHCHRKLQRGRLYD"
     gene            complement(1130..1567)
                     /locus_tag="VFX87_06140"
     CDS             complement(1130..1567)
                     /locus_tag="VFX87_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4994527.1"
                     /translation="MMAKRILVPLDGSESAEVAASLAADLARGSGGDVRLLQVYPVPE
                     HRVSNTGRVVAYADQEMERLSAEGRDYLESVEAQLAPVSTETVVRFGEPEQEILVEAE
                     AFDADMIVVTASRRSWLGRLIGSGLGEKLLHGSPVPVVVVRAA"
     gene            1696..2049
                     /locus_tag="VFX87_06145"
     CDS             1696..2049
                     /locus_tag="VFX87_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994528.1"
                     /translation="MIEIAVASVVGLVLVVGPLLWRVARDRSESRALSIRAYVQSLVD
                     RKLGGESFVSVQVTPRGLWGPGQVVVAVPGGWEWLLEEVWPVLLARTPRGYELVVRRG
                     ESTAPASQRQLKRVA"
     gene            2108..>3167
                     /locus_tag="VFX87_06150"
     CDS             2108..>3167
                     /locus_tag="VFX87_06150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994529.1"
                     /translation="MHVGMAAVFQNPGRAQSDRDVYVNELRLAELAEPLGFESVWGVE
                     HHFTDYTMCPDVLQFLSYMAGRTRNVQLGSMVVVLPWHDPMRVAEEVSMLDNLSGGRL
                     ILGLGRGAGKVEFDGFRLSMDESRARFVECAEMLLRGLEQGYCEYAGAFVKQPRAAIR
                     PAPFKSFRGRTYAAAVSPESVRIMAELGVGILIIPQKPWKEVARELEAYREVYRQMNG
                     VDAPPPISAGWTFCDASSARAEEMARRYIGGYYQTVLDHYQFQGDHLARTKGYEYYGK
                     MAEKIHQYGTETVIDYFMNLQVWGTPEQCYEKILDIHARTGNSHYVGVFSYAGMPYDE
                     AERNLRLFAKEVMPQLQKL"
BASE COUNT          471 a         1092 c         1116 g          488 t
ORIGIN      
        1 tggcttcgcg cgggctgaag cgcgcgacga tgtcccgctc gcgccggcta tagacctgca
       61 cgtccaccgt gaggcacacc gagaggtagc ccgaggcttc cacccgcgcg agcaggtcgg
      121 ccacccaggc ccggtcgccc atgtggtaaa gctggaacat cttggggccg ggcgccgcct
      181 ccgccaccgc ctcgaccgac cagccggtgg cgccgctcag gaactgtagc gtgtcgccct
      241 tggcggctcc gcgcgccatc tcgcgatcgc cctcgggatg gaagaggacg agcccgccca
      301 tgggcgcgat caccaccggc caggagagcg ggatgccgat cagcgacgtg gagagatcaa
      361 tgtggcggac gtccaccagg atgtcctggc ggacggccag gcgccggatc gcgcgccggt
      421 tgcggcgcag cgtggtctcg gtctcggtgc cgcccgcgcc gaagttgtac gagttgcgcg
      481 gcaggcgggc cttggccgcg cgatggatct ccgggagcgt gacgaagggc tcggtcatgc
      541 cgggtaacat aacatgccgc tgaagccggt gccgtaccgg agcctgcggg ccctgctgcg
      601 gcgggagctg atcggcgagg agcacgaggc gacggccgcc ctcatccggc ggctggccca
      661 cgtcaagagg atgggccgct tcacgcgcgc ggagttcctc acgatgtgcc ggtggaagag
      721 cccgcgctcg cgacggcact acgagcgcaa caccgcgggg acgatccggc gcgtgtcgag
      781 cgcggtgctc gccacgcgga gcgagcgcga gcgcatggcc caactcatcg cgctacccgg
      841 ggtgagcgtg gcgaccgcct ccgccatcct gaccctgatc gacccgcgcc gctacggggt
      901 gctcgacatc cgctgctggc agcttctctt cagtatccgc tcggtggcgg ggaacgagcg
      961 cgggcgggcg ttcacggtgt cacagtggga gcactacctg gagcggctgc ggggccacgc
     1021 gcgcgccctg ggcgtctccg cgcggatgat cgagtacacc ctcttccact gccaccggaa
     1081 gctgcagcgc gggcggctct atgactgacg ccgcccgcgc gagctcggat caggcggcgc
     1141 ggaccaccac gaccggcacc ggcgagccgt gcagcagctt ctcgccgagc ccactgccga
     1201 tgaggcgccc gagccagctc cggcgggacg cggtcaccac gatcatgtct gcgtcgaagg
     1261 cttccgcctc cacgaggatc tcctgctcgg gctccccgaa ccgcaccacg gtctcggtgg
     1321 acaccggggc gagctgcgcc tcgacgctct cgagataatc gcggccctcg gcgctcaggc
     1381 gctccatctc ctggtccgcg taggccacca cgcgcccggt attgctcacg cggtgctcgg
     1441 ggaccgggta cacctgcagc agccgcacat cgccgccgct gccgcgggcc aggtcggccg
     1501 ccagggaggc cgctacctcc gcggattcac tgccgtccag cggaacaaga atgcgctttg
     1561 ccatcatgat tctctccttc cacacgccct ttcgatggcg gattgtcgcc gatggattcc
     1621 tcctttctct gtcggtaatt gccagaatcc ctcggggagg cccatgcatc tcaatccgcg
     1681 gaaggagaga tcgccatgat cgagatcgcg gtggcctcgg tggtcgggtt ggtgctggtg
     1741 gtcggaccgc tcctgtggcg ggtggcccgg gatcgcagcg agtcccgcgc cctgtcgatt
     1801 cgcgcctatg tccagtccct cgtggaccgg aagctgggcg gcgagtcgtt cgtgtcggtg
     1861 caggtgacgc cgcgcgggct gtggggcccc gggcaggtgg tcgtggccgt ccccggcggc
     1921 tgggagtggc tgctggaaga ggtctggccg gtgctgctgg cgcgcacgcc gcgcggctac
     1981 gagctggtcg tgcgccgcgg cgagtccacc gcgcccgctt cgcagcggca gctcaagcgc
     2041 gtcgcctgac ccgcgcttgc tggtcgggtt ccccctcgcg taacatccgg gcgaggggga
     2101 acccaccatg catgtcggga tggccgccgt cttccagaac ccgggccggg cgcagagcga
     2161 tcgcgacgtc tatgtgaacg agctgcgcct ggccgagctg gccgagccgc tcgggttcga
     2221 atcggtgtgg ggcgtggagc atcacttcac ggactacacc atgtgccccg acgtcctgca
     2281 gttcctctcc tacatggccg gccgcacccg gaacgtccag ctcggctcca tggtggtggt
     2341 gctcccgtgg cacgacccga tgcgggtggc cgaggaagtg tccatgctcg acaacctgtc
     2401 gggcggccgc ttgatcctgg ggctcggccg cggggcgggc aaggtggagt tcgacggctt
     2461 ccgcctctcc atggacgaat cgcgcgcgcg cttcgtggaa tgcgcggaga tgctgttgcg
     2521 cgggctggag cagggctact gcgagtacgc cggcgccttc gtcaagcagc cgcgggccgc
     2581 gatccggccg gccccgttca agtctttccg cgggcgcacc tacgcggcgg cggtctcgcc
     2641 ggagtcggtg cggatcatgg ccgagctggg cgtcggcatc ctgatcatcc cccagaagcc
     2701 gtggaaggaa gtcgcgcgcg agctggaggc gtatcgcgag gtctaccgcc agatgaacgg
     2761 ggtggacgcg cccccgccga tctccgcggg ctggaccttc tgcgacgcga gctcggcccg
     2821 cgccgaggag atggcgcgcc gctacatcgg cggctactac cagaccgtgc tcgaccacta
     2881 ccagttccag ggcgatcacc tggcccggac caagggctac gagtactacg gcaagatggc
     2941 ggagaagatc caccagtacg gcaccgagac cgtcatcgac tacttcatga acctgcaggt
     3001 ctggggcacg ccggagcagt gctacgagaa gatcctggac atccacgcgc gcaccggcaa
     3061 cagccactac gtgggcgtgt tcagctacgc gggcatgccc tacgacgagg ccgagcgcaa
     3121 cctgcgcctc ttcgccaagg aagtgatgcc gcagctccag aagctcg
//
LOCUS       DASUFA010000173         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_612594, whole genome shotgun sequence.
ACCESSION   DASUFA010000173 DASUFA010000000
VERSION     DASUFA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612594"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFX87_06155"
     CDS             <1..964
                     /locus_tag="VFX87_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4994530.1"
                     /translation="APGIRWIRMPLPFQLNHINLWLLDDEAGVTVVDTGIGLPPTREL
                     WEKLFTGELAGRPLVRVLVTHFHPDHIGNADWLARRFGVELWCPQAEWLMAHLAWQGM
                     GGNDSEKRLNHYRRHGVPPEQAEAFRSLGNNYRKLVPSVAPSFRAVREGDVVTIGGRR
                     WHAFTVMGHAPEHACLFSPEANVLISGDQVLPKITTNVSVWPDQPNGNPLKLYLDSLG
                     RFRPLPAETLVLPSHGLPFIGLHKRLAHLQHHHDERLSEAIDALAEPRTAHELVPVLF
                     RRQLDTHQLGFAIGETVAHLHFLEARGQVARLVDGRGVHRFRKA"
     gene            970..1227
                     /locus_tag="VFX87_06160"
     CDS             970..1227
                     /locus_tag="VFX87_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994531.1"
                     /translation="MANEHGHLPKADPERLDPELRRRFEVWRAKAYQDDNLFLTLARR
                     PAVLDLFLAWVGFIYAGGASLDPALVELCRIRLAQRNRCVH"
     gene            1249..1545
                     /locus_tag="VFX87_06165"
     CDS             1249..1545
                     /locus_tag="VFX87_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994532.1"
                     /translation="MVRKGLTEREVQQALGDLDHAELPARTVAALRLADTLSGERPRV
                     DDALFAVLRQHFDEGQILELGAALTVASGWQRLIEAFGIRPDAWNETTPLPWPR"
     gene            complement(1553..2494)
                     /locus_tag="VFX87_06170"
     CDS             complement(1553..2494)
                     /locus_tag="VFX87_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX4994533.1"
                     /translation="MNIHQNARLTLRGREEAVRRVTQLGHSARQVARALHTTDTTVRK
                     WVARAAAGESLADRSSRPQHSPQATAPAVVLRIQVLRQQRRTCGEIAAQVAVSRATVA
                     RIVRRCGWARLHVLELPPTWRRYERAQPGELLHLDTKKLGRIVRPGHRVTGDRRDTVD
                     GAGWEFGHMAVDDASRVALGEVLPDERGGTAAGFLQRTVRGLRRLGVRVAAVMTDNGA
                     GYRSRRFRATCQRLGLRHLFTRPYTPRTNGKVERFIKTALLEWAYARLYRHSDERRRA
                     LALWLHHYNWHRPHTSLGGRPPITRVLTATNLVRLHS"
     gene            complement(2599..3330)
                     /locus_tag="VFX87_06175"
     CDS             complement(2599..3330)
                     /locus_tag="VFX87_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX4994534.1"
                     /translation="MAKTAGIVLIGNELLSGKIADANAAYLCRELRALGVEVRRISVI
                     PDEVDLIAAEVREQSAAHDVVFTSGGVGPTHDDVTMEGVARAMGTRVIRHPSLVALLE
                     GYYKGQLNEARLRLAEVPEGAELVADGSLMFPAVTMRNVYILPGVPEIFRQKFDALRE
                     RFREMPFHLISVFVNIGEGTLADHLNALVARHPELLLGSYPEFSNPEYKVKITFESKD
                     RTLVERAVEELLAALPPGALVRVQR"
     gene            3380..>3538
                     /locus_tag="VFX87_06180"
     CDS             3380..>3538
                     /locus_tag="VFX87_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX4994535.1"
                     /translation="MALPTSEPVRLSGWALVLGASSGFGEATSLSLARAGLHVFGVHL
                     DRRATLPNV"
BASE COUNT          506 a         1275 c         1230 g          527 t
ORIGIN      
        1 ggcgcccggc atccgctgga tccggatgcc gctgcccttc cagctcaacc acatcaacct
       61 gtggctgctc gacgacgagg ccggggtgac ggtggtggac accgggatcg ggctgccgcc
      121 gacgcgcgag ctgtgggaga agctcttcac gggcgagctg gccggacggc ccctcgtgcg
      181 cgtgctcgtc acccatttcc acccggatca catcggcaat gcggactggc tcgcccggcg
      241 cttcggggtc gagctctggt gcccgcaggc cgagtggctc atggcccacc tggcctggca
      301 gggaatgggc ggcaacgact cggagaagcg gctcaaccac tatcgccgtc acggggtccc
      361 gcccgagcag gccgaggctt ttcgcagcct cggcaacaac taccggaagc tcgtgccctc
      421 ggtcgcgccc agcttccgcg cggtgcgcga gggcgacgtc gtcacgatcg gcgggcgccg
      481 gtggcacgcc ttcacggtga tggggcacgc gcccgagcac gcgtgcctct tctcgcccga
      541 ggcgaacgtg ctgatctcgg gcgatcaggt gctgcccaag atcacgacca acgtgagcgt
      601 gtggccggac cagcccaacg gcaatccgct caagctctac ctggactcgc tcgggcgctt
      661 ccggccgctg cccgcggaga cgctggtgct gccgtcgcac gggctgccct tcatcggcct
      721 gcacaagcgg ctcgcccacc tgcagcacca ccacgacgag cgcctcagcg aggcgatcga
      781 cgccctcgcc gagccgcgga cggcccacga gctggtgccg gtgctcttcc gccggcagct
      841 cgacacgcac cagctcggct tcgccatcgg cgagaccgtc gcgcacctgc acttcctgga
      901 agcgcgaggc caggtcgccc gcctcgtgga cggccggggc gtgcaccgct tccgcaaggc
      961 ctagcgtcca tggccaacga gcacggccac ctgcccaagg ccgatcctga gcggctcgat
     1021 ccggagctgc ggcgccgctt cgaggtgtgg cgcgccaagg cgtatcagga cgacaatctc
     1081 ttcctcacgc tcgcgcgccg ccccgcggtg ctcgacctgt tcctcgcctg ggtcggcttc
     1141 atctacgcgg gcggcgcgag cctcgatccc gcgctggtgg agctctgccg catccgcctc
     1201 gcccagcgca accggtgcgt gcactgaagc ctggcgcgca gcgcatcgtt ggtgcgcaag
     1261 ggcctgaccg agcgggaggt acagcaggcg ctcggcgatc tcgaccacgc tgagctgccc
     1321 gcccgcacgg tggcggcgct gcggctcgcc gacaccctga gcggcgagcg gccccgggtg
     1381 gacgacgcgc tcttcgcggt gctccggcag cacttcgacg aggggcagat cctggagctg
     1441 ggcgccgcgc tcaccgttgc gtccggctgg cagcgcctga tcgaggcgtt cggcatccgg
     1501 cccgacgcgt ggaacgagac gacgccgctg ccctggccgc gctgatcggc ggctagctgt
     1561 ggagtctcac gaggttcgtt gcagtcagga cccgggtgat gggggggcga ccgccgaggc
     1621 tcgtgtgggg acgatgccag ttgtagtgat ggagccagag ggccagggcg cggcgccgct
     1681 cgtcggagtg gcggtacagg cgcgcatagg cccactcgag caaggcggtc ttgatgaagc
     1741 gctcgacctt gccgttggtc cgcggggtat agggccgggt gaagagatgg cgcaggccga
     1801 gccgctggca ggtcgcccgg aagcgccgcg agcgataccc ggcgccgttg tcagtcatga
     1861 cggccgccac gcgcaccccg agccggcgca ggccccgcac cgtgcgctgg agaaagcccg
     1921 cggcggtgcc cccgcgctca tcgggcaaga cttcgccgag ggccacgcgc gaggcatcgt
     1981 cgacggccat gtggccaaac tcccagccgg ccccgtcgac ggtgtcccgc cgatcgccgg
     2041 tgacccgatg gccggggcgg acaatgcgcc ccagcttctt ggtgtccagg tgcagcagct
     2101 cgccgggctg ggcccgctcg tagcggcgcc aggtcggcgg cagctccaac acgtgcagcc
     2161 gcgcccagcc acagcggcga acgatgcgcg cgaccgtcgc ccgactcacg gccacctgcg
     2221 cggcgatctc gccacacgtc cgccgttgct ggcgcaggac ttggatccgc aggaccaccg
     2281 ccggggcggt cgcctgcggg ctgtgctggg ggcgggagct gcgatccgca aggctctcgc
     2341 ccgccgcggc gcgcgccacc cacttccgga ccgtggtgtc ggtggtgtgc agggcccggg
     2401 cgacctgccg cgcggagtgg cccagctggg tcacccgccg gacggcttcc tctcgacctc
     2461 gcagcgtcaa tcgggcattc tggtggatgt tcatccgggg atcctcctca gcgatctgga
     2521 gtgcttggca gcgtccagct tcgttgagag cccccggatg gacaaccccc ctgcaaagaa
     2581 cctcgtgaga cttcacagct agcgctgcac tctcaccagg gcccccggtg gcagcgcggc
     2641 gagcagctct tccacggcgc gctccacgag ggtccggtcc ttggactcga aggtgatctt
     2701 caccttgtac tcgggattgg aaaactccgg gtatgaaccg aggagaagct cggggtggcg
     2761 ggcgaccagc gcgttgaggt ggtcggccag cgttccctcc ccgatattca cgaacacgct
     2821 gatcaggtgg aacggcatct cccggaaccg ctcgcgaagg gcgtcgaact tctggcggaa
     2881 gatctcgggg acgccgggga ggatatagac gttccgcatg gtcacggcgg gaaacatcag
     2941 cgagccgtcg gcgaccagct cggccccttc cggcacctcg gccaggcgga gccgcgcctc
     3001 attgagctgg cccttgtagt agccctcgag caaggcgacc aggctcgggt ggcggatgac
     3061 ccgcgtcccc atcgcccgcg ccacgccctc catggtcacg tcgtcatggg tggggcccac
     3121 gccgcccgac gtgaacacga cgtcgtgggc ggcgctctgc tcccgcacct ccgccgcgat
     3181 gaggtccacc tcgtccggga tcaccgagat ccggcgcacc tccacgccca gcgcgcgtag
     3241 ctcgcgacac aggtacgcgg cattggcgtc ggcgatcttg ccggacagga gttcgttgcc
     3301 gatcaggacg atgccggcgg tcttcgccat ggcgcccaat ggtagcacgg cggcgtgtgg
     3361 tgttagaatg gccgccgaga tggcccttcc caccagcgag cccgtccggc tgtccggctg
     3421 ggccctcgtt ctcggcgcgt cctccggctt cggcgaggcc accagcctct cgctggcccg
     3481 cgccggactc cacgtcttcg gcgtgcacct cgaccgtcgc gcgaccctgc ccaatgtc
//
LOCUS       DASUFA010000174         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_148697, whole genome shotgun sequence.
ACCESSION   DASUFA010000174 DASUFA010000000
VERSION     DASUFA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148697"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFX87_06185"
     CDS             <1..300
                     /locus_tag="VFX87_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4994536.1"
                     /translation="ARFDLLITPTVAVPAFEVGKPGLNEVNGQPSSPLGWTPFCFPFN
                     LTGQPAATVPVGFTSAGLPVGLQIVGRRHADHTVLAASAAFEAAQPWADRRPPVD"
     gene            308..685
                     /gene="crcB"
                     /locus_tag="VFX87_06190"
     CDS             308..685
                     /gene="crcB"
                     /locus_tag="VFX87_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX4994537.1"
                     /translation="MHFALVCLGGAVGTGARYLLGGLAIRWLGGEFPYGTLLVNVIGS
                     FLIGLVQELAFGAAVVPENVRVVLAVGVLGGFTTYSAFAYESMRLAAVGSWGPAVLYV
                     GLTTALCFAACLVGIGAARLVAG"
     gene            complement(682..900)
                     /locus_tag="VFX87_06195"
     CDS             complement(682..900)
                     /locus_tag="VFX87_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994538.1"
                     /translation="MTRESDRARRVFQALILVVAMAVAFLGGMLMERLQSHAERDAML
                     RRYDQALREHRARVMEAEKRGEETTRRP"
     gene            979..1374
                     /locus_tag="VFX87_06200"
     CDS             979..1374
                     /locus_tag="VFX87_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994539.1"
                     /translation="MWRRARGTSRAVLAALLIYVGAGAAIAQVQTRSVDSDLRVEWTG
                     SEDRRGRPVVSGYVYNQRAGSYAVSVRLLVDALDGSGQVVGSSTGYILGDVPPSNRAY
                     FELGAPAKAASYRVTIVSFAWRAYGAGGG"
     gene            1382..2494
                     /locus_tag="VFX87_06205"
     CDS             1382..2494
                     /locus_tag="VFX87_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEX4994540.1"
                     /translation="MATRWRFALALCPVTVWVLLWGAAVVALAAPGPDDPYLAGYAGA
                     VLEREFKVPRRAVTVDNGVLTLDGAQLGGADRVALLATLSNLPGVVRVEVREAAAAAP
                     AVVPDVAPAAVSALPTGLLPTGHLFQSLLADPRWPHFSAAYRYYLGHPELGHVGAVSF
                     GETIPLFRGNGFADSQWEAGIQAGVFALFQMDAPSKDLLNADYFASVFGTWRRGPVSV
                     LGRVFHQSSHLGDELLLRTRLERINLTYESVDLKLSYDLPWGFRVYGGGGYLFDQEPA
                     SLNPWAAQGGVEFRSPWTIAAGYVRPVVAVDLQSRDENKWNLDLSVRGGIQFENVRVL
                     ERNLQLLVEYFNGNSSDGQFYKRRVEYLGLGAHFHF"
     gene            complement(2576..3079)
                     /locus_tag="VFX87_06210"
     CDS             complement(2576..3079)
                     /locus_tag="VFX87_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4994541.1"
                     /translation="MLTVETLCRTLGLVPHPVEGGYFAETYRSADHIPASGLPSRYGA
                     ARAASTAIYYLLTPDTFSAMHRLASDEIFHFYRGDPVEMLHLHPDGSHQVVVLGPDLE
                     AGQRPQVVVPRHIWQGARLIPGGRYALLGTTVAPGFDYADYETAPRPTLLATHPAAKD
                     LIRALTR"
     gene            3101..3550
                     /locus_tag="VFX87_06215"
     CDS             3101..3550
                     /locus_tag="VFX87_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4994542.1"
                     /translation="MPRLRRIMHATDFSRASTAALKRAVTMAKADRAQLIVVHVMTPP
                     SLALPGDGYISPSLYEDLEASARTEAQKRLNAIVAGARKAGARAAGLLLEGMPHERVT
                     RAARARKADLLVIGTHGRTGLAKLFLGSVATRLVAGAPCPVLTVRGR"
BASE COUNT          459 a         1265 c         1395 g          588 t
ORIGIN      
        1 gcgcgcttcg atctcctgat cacgcccacg gtcgcggtgc ccgcgttcga ggtcggcaag
       61 cccgggctca acgaggtcaa cgggcagccg tcctcgccgc tcggctggac gccgttctgc
      121 ttcccgttca acctcaccgg ccagcccgcc gccaccgttc cggtcggctt cacgagcgcg
      181 gggctgccgg tggggctgca gatcgtcggg cgccgccacg ccgatcacac cgtcctggcc
      241 gcgtccgccg ccttcgaggc cgcgcagccc tgggcggacc gccggccgcc cgtcgactga
      301 tcgccccgtg cacttcgccc tcgtctgcct gggcggtgcg gtggggacgg gggcccggta
      361 cctcctgggc ggtctcgcca tccgctggct cggcggcgag ttcccctacg gcaccctgct
      421 cgtcaacgtg atcgggtcgt tcctcatcgg cctggtccaa gagctggcct tcggcgcggc
      481 ggtggtgccg gagaacgtgc gggtggtgct ggcggtcggg gtgctgggcg gcttcaccac
      541 ctactcggcg ttcgcctacg agtcgatgcg gctggccgcg gtgggctcgt gggggccggc
      601 ggtgctctac gtcgggctca ccaccgcgct gtgcttcgcg gcctgtctgg tgggcatcgg
      661 ggcggcccgt ctcgtggccg gttaaggcct ccgggtagtt tcttcccccc gcttctccgc
      721 ctccatcacg cgggcccggt gctcgcgcag cgcctgatcg taacgtcgca gcatcgcatc
      781 ccgttcggcg tgggactgaa gccgctccat cagcatcccg cccaggaagg ccaccgccat
      841 cgccaccacg aggatcagcg cctggaagac ccggcgggcg cggtcggact cgcgcgtcat
      901 gggccgcatg atagcaaatg ccttgcgagc ccgaattcga tggtggcgag ttgcgtccgg
      961 ggtgacaatg gcgggacgat gtggcgacgc gcgcgaggca cgagcagggc ggtcctggcc
     1021 gcgctgctga tctacgtggg cgcgggcgcc gcgatcgcgc aggtgcagac gcgatcggtt
     1081 gacagcgatc tgcgcgtcga atggaccggc tcggaggatc gccgcggccg tccggtggtg
     1141 agcggctacg tgtacaacca gcgggcgggc agctacgcgg tgagcgtccg gctgctcgtg
     1201 gacgcgctcg acggctcggg ccaggtggtt ggctcgagca ccggctacat cctgggtgac
     1261 gtgcccccgt cgaaccgggc ctatttcgag ctcggggctc ccgcgaaggc ggcgagctat
     1321 cgggtcacga tcgtgtcgtt tgcctggcgg gcgtacggcg ccgggggagg atagagagct
     1381 catggcgacc cgttggcggt tcgcgctggc cctgtgcccg gtcaccgtgt gggtgttgct
     1441 gtggggcgcg gcggtggtcg ccctcgccgc gcccgggccg gatgacccgt acctggcggg
     1501 ctacgcgggc gcggtgctcg agcgcgagtt caaggtcccc cgccgcgcgg tgacggtgga
     1561 caacggggtc ctcacgctcg acggcgcgca gctggggggc gccgatcgcg tcgccctgct
     1621 ggcgacgctc tcgaacctgc ccggcgtggt ccgcgtcgag gtgcgcgagg cggccgccgc
     1681 cgcgcccgcg gtcgtgcccg acgtggcgcc ggccgcggtg tcggccctgc ccaccgggct
     1741 gctgccgacc gggcacctct tccagtctct gctggcggat ccgcggtggc cgcacttctc
     1801 ggcggcctac cgctactacc tgggccaccc ggagctgggg cacgtgggcg cggtgagctt
     1861 cggcgagacc atcccgctgt tccggggcaa cggcttcgcg gacagccagt gggaggcggg
     1921 gatccaggcc ggtgtgttcg cgctcttcca gatggacgcg ccgtcgaagg atctgctcaa
     1981 cgccgactac ttcgcctccg tgttcggcac ctggcgccgg ggcccggtct ccgtgctcgg
     2041 gcgcgtcttc caccagagct cgcacctggg cgacgagctg ctgctccgca cgcgcctcga
     2101 gcggatcaac ctcacctacg agagcgtgga tctgaagctc tcctacgatc tgccgtgggg
     2161 cttccgggtt tacggcggag gcgggtacct gttcgaccag gagcccgcga gcctcaatcc
     2221 gtgggccgcg cagggcggcg tggagttccg cagcccgtgg acgatcgcgg ccggctatgt
     2281 gcggccggtg gtggcggtgg acttgcagtc tcgggacgag aacaagtgga acctggatct
     2341 gtcggtgcgc gggggcatcc agttcgagaa cgtgcgcgtg ctcgagcgga acctccagct
     2401 gctcgtcgag tacttcaatg gcaactcgtc cgacgggcaa ttctacaagc ggcgcgtcga
     2461 gtacctcggg ctgggggcgc acttccactt ctagctctcg gccggtcgtg ggcagagacc
     2521 cgccccccgc cgtccccgcc cgcccggcgg gatcgctcgg tggcctctga atccgtcatc
     2581 tggtcagggc gcgtatcagg tcctttgcgg cggggtgggt ggccaggagg gttgggcggg
     2641 gggcggtttc gtagtcggcg tagtcgaagc cgggggccac ggtcgtcccg aggagcgcgt
     2701 accggccgcc ggggatcaga cgggcgcctt gccagatgtg acgcgggacg accacctggg
     2761 ggcgctggcc ggcttcgagg tcggggccga gcactacgac ctggtgggag ccgtcggggt
     2821 ggaggtggag catctcgacc gggtcgcctc gatagaagtg gaagatctcg tcggaggcga
     2881 ggcggtgcat ggccgagaac gtgtcggggg tcagcaggta gtagatcgcg gtgctggcgg
     2941 cgcgggcggc gccgtagcgg gacggcaggc cgctcgcggg gatgtggtca gcgctccggt
     3001 aggtctcggc gaagtagccg ccctccaccg ggtgcgggac caggccgagg gtccggcaga
     3061 gtgtctcgac ggtgagcatg gcgcattgta agatacggcc gtgccacgcc tgcgacggat
     3121 catgcacgcc acggatttct cacgcgcctc caccgcggcc ctcaagcgcg cggtcacgat
     3181 ggcgaaggcc gatcgggccc agctcatcgt cgtacacgtc atgacgccgc cctcgctggc
     3241 gctgccgggc gacgggtaca tctcgcccag cctctacgag gacctcgagg cctcggcacg
     3301 caccgaggcg cagaaacgcc tcaacgcgat cgtggcgggg gcgcggaaag cgggcgcgcg
     3361 cgccgccggg ctcctgctgg agggcatgcc ccacgagcgc gtcacccggg cggcgcgggc
     3421 caggaaggcc gacctgctcg tgatcggcac ccacgggcgc accgggctcg ccaagctctt
     3481 cctcggcagc gtggcgacgc ggctggtcgc cggcgcgccg tgcccggtgc tcaccgtgcg
     3541 gggccggtga ccgcgatggc cggcgcgatc accgccgatt cgaagatcaa cgacgtgctg
     3601 gcgaagcatc ccgcgaccgc gccggtcatc acccagggcc gccggctcta cgtggaccag
     3661 ccgcgcgagc tctacgcgcg gtttcccggc ctgagcgtgg gcgactt
//
LOCUS       DASUFA010000175         9500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1409023, whole genome shotgun sequence.
ACCESSION   DASUFA010000175 DASUFA010000000
VERSION     DASUFA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409023"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFX87_06220"
     CDS             <1..396
                     /locus_tag="VFX87_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4994543.1"
                     /translation="VFGVFCRRWGVPLLDGGTVRLPHLIGLSRALDMILTGRPVDAHE
                     ALAFGLANRVVPTGRAREEAEALARAIAAFPPHCVRSDRRSAWESAGLPLPAAMASEF
                     RLGKATHDTGESQAGARRFAAGAGRHGQF"
     gene            425..1435
                     /locus_tag="VFX87_06225"
     CDS             425..1435
                     /locus_tag="VFX87_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX4994544.1"
                     /translation="MADRPFKVYVIQGRGERDYADCQTALDRVPAQMISLPFIGDADE
                     VIARTRDADALIISSSPVTRRVMESLEGVKAVMRTGVGYDVIDVAAATELGVVVINVP
                     DVWIREVANHALALLLAWNRRIVTLDREVHAGTWSSRVPGAVTGSLHGETVGIVGLGN
                     IGSAFARRIAALETHVIACDPYVDDARFKALGVERVDLPTLAARADYVSVHALLNAET
                     RGLINEAFFRRMKPTACLVNTSRGPVVDEAALVRALQERKLAGAALDVFEQEPLAADS
                     PLRRMENVILTPHAAYFSSPAVAQVPKRCGEEIARVLLKERPINVVNPEVYAPGRARR
                     GR"
     gene            1454..2290
                     /locus_tag="VFX87_06230"
     CDS             1454..2290
                     /locus_tag="VFX87_06230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4994545.1"
                     /translation="MHLGVFSYNVEYGARPDELARAAEDRGFESFWVGEHTHIPAARQ
                     TPYPGGEPLPKPYYHMADPFVSLMAAAAATRTIKLGTGICLVVEHDPIVLAKAVATLD
                     WLSRGRVLFGIGGGWNREEMEHHGTPFAKRWRVLRERVLAMKALWTQEEASFHGEFVT
                     FDRAISFPKPVQTPHPPILFGGATAQGRARVVEYCDGWIPIDVLLDDLPAAIADLRRR
                     AQAAGRRPDELSVSVFAFKPPTADAVKRMEDMGVVRVTMVAPRRLDDALLFLDRMATL
                     AA"
     gene            2292..3131
                     /locus_tag="VFX87_06235"
     CDS             2292..3131
                     /locus_tag="VFX87_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4994546.1"
                     /translation="MPELDARTVEAYWGRENLAEAILAALTARGTDLDALTPDALAPV
                     DQFHGGGKPVTERLARLAGAGPGMRVLDVGGGFGGPARTLAVEFGCLVTTVDLTASYV
                     RAAQALTTRMRLDDRVSHHVGNALALPFPDGTFDLVWTQNTGMNIADKERLYAGFRRV
                     VRPGGRLALQEPMAGPAQPLLYPVMWARDPSTSFLRTPAEMRAVIETAGFRARVWDDV
                     TAETAGPATAATIPAHSIQRLVMGGAIDEIIRIGHRNRDEGRLVSIQAVFERPLDKTE
                     PAG"
     gene            3143..4489
                     /locus_tag="VFX87_06240"
     CDS             3143..4489
                     /locus_tag="VFX87_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4994547.1"
                     /translation="MSDALTGQTVELLQQMIRNRCVNDGTLLSGQEKRNSDTLRSYLA
                     GSGLDMEVYEPAHAPGRASLVARIEGTDPKAPTLCLMGHTDVVPVNPKTWTRDPFGGE
                     LVDGEVWGRGAVDMLNLTASQAVALKTLAGRGWRPRGTLVYLACADEEAGGTLGAGHV
                     VERHWDALRADCCLTENGGTVSRGARGDELNVTVHVGEKGVAWRRLRVKGTPGHGSMP
                     YGANNALIKAAQVVSRLADYRPSPYVDELWRAFVDTLHLDPAMKEALADPARVDESIE
                     GLAPSLAKLAWSATHTTFSPNVCHGGVKTNVIPDAVDVEVDIRTVPGDNDDEVRRHLD
                     KALGGLAEEIEVEKLFSKPASMSPTGTPLWHVLGRVVDAHYPGAKLLPKMIVGFTDAP
                     YFREKGAVAYGFGLFSRTLTAEAMSGRFHGNDERIDVESLALTTQAWLEVCEQFLG"
     gene            4490..6679
                     /locus_tag="VFX87_06245"
     CDS             4490..6679
                     /locus_tag="VFX87_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX4994548.1"
                     /translation="MIPITGYSDRLSAAPGERIAFKVSSAATGPYRASLARVIHADPN
                     PAGPGLKIEDLAARFTIERPSRAQPLAQGSHVRVDAAAAVHTPGPLTVTALVWPSFAA
                     KREQCVISRWDETGQAGWALSLTSGGVTARVGAPGGAPVTVTTGQALPLRRWHRIWLV
                     ADAAAGTLRVGHAPLTGGGREEASAPLPPAARLDAAAPLLIGARLAREAREHYNGKIE
                     DPMLLAAAAAAPETLLLDPLLPPDGLIAGWDFSQGIAGLDVTDVGPHRLGGRLVNLPT
                     RAVTGARWTGREMCWRHAPREYAAIHFHDDDVYDAGWATDFEFTVPDDLPSGAYLMRL
                     SAEGHADDLPFYVRPARGRPRADVLYIASTYTYQAYANHARGSSDAGYRERVAAWGAY
                     PHAPDNHPDYGWSTYNRHRDGSGICYSSRLRPVLTFRPRYLTFLDARGSGLRHYSADT
                     HLLDWLEAQGIRYDVVTDEDVEAEGAALLAPYATVLTGSHPEYHTARTLDAHAGYLDG
                     GGKLAYLGGNGFYWRIATSRAVPGVIEVRRAEGGIRAWDAQTGEYYHALDGAYGGLWR
                     RNGRPPQRIGAVGFSAQGLFEGSYYRRQPGADDPKAAWILDGVEDAVIGDFGLSGGGA
                     AGFELDRADPALGTPAGAIVVASSEGHGPSFIVVPEELLSHLATVSGEKPARLLRADM
                     TYAELPGGGAIFAVGSITFCGSLSHNRYDNNVSRILRNVLDRFRARR"
     gene            6676..7437
                     /gene="yqeC"
                     /locus_tag="VFX87_06250"
     CDS             6676..7437
                     /gene="yqeC"
                     /locus_tag="VFX87_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HEX4994549.1"
                     /translation="MKLTDALQMTADEVVALVGGGGKTTAMFRLAREVVDGGGRAITT
                     TTTRIFGAQIALAPAHVPAVDLTRERVAAALAAHRHVLVIGETDAGSGKAEGVSLDLF
                     RRLRAWFPAVCLLNEADGSRMRPFKAPADYEPVIPTETTLVVPVVGADVFGKPLDADH
                     VHRPELVGALCGAPLGTPITPSIVARVLAHPRGGRKGVPAGARVVVLINKVEALPDHA
                     AARETAACLLREPAIHSVVLANLRAEDPVLEVCAR"
     gene            7434..8606
                     /locus_tag="VFX87_06255"
     CDS             7434..8606
                     /locus_tag="VFX87_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4994550.1"
                     /translation="MTLDVPHLRADTPGVANVLHFNNAGAALPPRPVLAAVTGHLERE
                     AAIGGYEAAAEAADRIADVYDAVAALIGAGPDEIAFVENATRAWDMAFYAIPFRAGDR
                     IVTARAEYASNYLAFLQMKRRAGVEIDVVDDDASGQLDVAALERAIRPRTRLIAITHV
                     PTQGGLVNPAAEVGWIAARHGILYLLDACQSVGQLTVDVARIGCHMLSATGRKYLRGP
                     RGTGFLYVRRDTIGALEPPFIDLEAASWIDADTYTLRDDARRFENWERFVAGQIGLGV
                     AVRYALALGIEAIEPRVKALADLLRRELAKRPGVTVHDLGTERCGIVTFLKAGEAPDA
                     TRRRLRALDINVTHTQARSSRLDLPARGFDALVRASVHYYNDEAEVERFVRAVSAS"
     gene            8622..>9500
                     /locus_tag="VFX87_06260"
     CDS             8622..>9500
                     /locus_tag="VFX87_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HEX4994551.1"
                     /translation="MTTVETALGPVATAELGATLMHEHIVTRSPGVQENWPHLWDRAG
                     ILAMAERKMADLHARGIRTIVDLTTVDLGRDIGLILDVARRSRVHVIVATGVWWMPQR
                     YFSGHEVDAVADLFVRDITQGIGDSGVKAAIIKCATDTAGVTPVIENILRAAARAQKA
                     TGVPISTHTWAAGRTGEAQQAIFAQEGVDLRRVIIGHSGDSEDLGYLRGLMDRGSTIG
                     MDRFGLEHFLPTPKRVEVIARLCAEGYAARMVLSHDANCWSDMLSEDDKRRTRPRWNY
                     NHISDDILPALRKAGVP"
BASE COUNT         1318 a         3571 c         3337 g         1274 t
ORIGIN      
        1 gtcttcggag tgttctgccg ccgctggggc gtgccgctgc tcgacggcgg gacggtcaga
       61 ctgccccacc tgatcggtct gtcgcgcgcg ctcgacatga tcctgaccgg ccgcccggtg
      121 gatgcccacg aggccctcgc cttcgggctc gccaatcgcg tggtccctac cgggcgggcc
      181 cgcgaggagg cagaggcgct ggcccgggcc atcgcggcct tcccgccgca ctgcgtgcgc
      241 agcgaccggc gctccgcctg ggagagcgcg ggcctccctc tgcccgccgc catggccagc
      301 gagttcaggc ttggcaaggc cacgcacgac accggcgaga gccaggcggg cgcgcggcgc
      361 tttgcggcgg gcgccggccg ccacgggcag ttctgaccga cacctctttc cgacggagag
      421 accagtggcc gaccgaccgt tcaaggtgta cgtcatccag ggccgcggcg agcgtgatta
      481 cgccgactgc cagaccgcgc tcgatcgcgt gcccgcccag atgatctcgc tgcccttcat
      541 cggcgacgcc gacgaggtga tcgcgcgcac ccgcgacgcg gacgcgctca tcatctcctc
      601 ctccccggtc acccggcgcg tgatggagtc gctcgagggc gtcaaggcgg tcatgcgcac
      661 cggcgtgggc tacgacgtca tcgacgtggc cgccgcgacg gagctgggcg tcgtggtgat
      721 caacgtgccc gacgtctgga tccgcgaggt ggccaaccac gccctcgcgc tgctgctggc
      781 ctggaaccgt aggatcgtca cgctcgaccg ggaggtgcat gccggcacgt ggagctcgcg
      841 ggtcccgggc gcagtcaccg gctcgctcca cggcgagacc gtcggcatcg tcggcctcgg
      901 caatatcggc agcgccttcg cccgccggat cgccgcgctc gaaacgcacg tgatcgcctg
      961 cgacccgtac gtggacgacg cccggttcaa ggcgctcggg gtcgagcgcg tggatctgcc
     1021 cacgctggcc gcgcgggcgg actacgtctc ggtccacgcg ctgctgaacg cggagacccg
     1081 cggcctgatc aacgaggcct tcttccgccg catgaagccg accgcctgcc tcgtcaacac
     1141 ctcgcgcggt cccgtcgtgg acgaggcggc cctcgtccgg gcgctccagg agcgcaagct
     1201 ggccggcgcg gcgctcgacg tcttcgagca ggagccgctc gccgccgaca gcccgctgcg
     1261 gcgcatggag aacgtgatcc tcaccccgca cgcggcctac ttctcgtctc ccgcggtggc
     1321 ccaggtgccg aagcgctgcg gcgaggagat cgcgcgcgtc ctgctgaagg agcgcccgat
     1381 caacgtcgtc aaccccgagg tctacgcgcc cgggcgggcg cgccgcggcc ggtaggtagg
     1441 gccggagccc accatgcacc tcggcgtctt cagctacaac gtggagtacg gcgcgcggcc
     1501 ggacgagctg gcccgcgccg cggaggaccg ggggttcgag tctttctggg tgggcgagca
     1561 cacccatatc ccggccgccc ggcagacccc gtacccaggg ggcgagccgc tgcccaagcc
     1621 ctactaccac atggccgatc cgttcgtctc gctgatggcc gcggcggccg cgacgcggac
     1681 gatcaagctc ggcaccggca tctgcctcgt ggtcgagcac gaccccatcg tcctcgccaa
     1741 ggcggtcgcc acgctcgact ggctttcccg cggccgcgtg ctcttcggca tcggcggggg
     1801 ctggaaccgg gaggagatgg agcaccacgg cacgccattc gcgaagcgat ggcgcgtcct
     1861 gcgcgagcgc gtgctggcca tgaaggccct ctggacgcag gaggaggcct cgttccacgg
     1921 cgagttcgtc accttcgacc gcgcgatctc gttccccaag cccgtccaga cgccgcatcc
     1981 cccgatcctg ttcggcggcg ccaccgccca gggccgcgcc cgcgtggtgg agtactgcga
     2041 cggctggatc ccgatcgacg tcctgctgga cgacctgccc gccgccatcg ccgatctccg
     2101 ccggagggcc caggccgcgg gccgccggcc ggacgagctc tcggtctccg tcttcgcctt
     2161 caagccgccc acggccgacg cggtgaagcg catggaggac atgggggtgg tgcgcgtcac
     2221 catggtggcg ccgcgccgcc tcgacgacgc gctgctcttc ctcgaccgga tggcgacgct
     2281 cgccgcctga gatgcccgag ctcgacgcca ggacggtcga agcctactgg gggcgcgaga
     2341 acctcgccga ggccatcctg gccgcgctca ccgcccgggg caccgatctc gacgcgctca
     2401 cgcccgacgc cctggcgccg gtggaccagt tccacggggg cggcaagccg gtcaccgagc
     2461 gcctcgcgcg actggccggg gccgggcccg gcatgcgggt gctcgacgtc ggcggcggat
     2521 tcggcggccc ggcccgcacg ctggccgtgg agttcggctg cctcgtgacg accgtggacc
     2581 tgaccgcctc ctacgtgcgc gcggcgcagg ccctgaccac gcggatgcgc ctcgacgacc
     2641 gggtgagcca ccacgtgggc aacgcgctgg cgctcccgtt ccccgacggg accttcgacc
     2701 tggtgtggac gcagaacacc ggcatgaaca tcgcggacaa ggagcgcctc tacgcgggct
     2761 ttcgccgggt cgtgcggccc ggcgggcgac tcgcgctgca ggagccgatg gccggaccgg
     2821 cgcagccgct gctctacccg gtgatgtggg cccgcgatcc ttccacgagc ttcctgcgca
     2881 cgccggccga gatgcgggcg gtgatcgaga ccgccggctt ccgggcccgc gtgtgggacg
     2941 acgtgaccgc ggagaccgcg ggaccggcca ccgcggccac gatccccgcg cacagcatcc
     3001 agcgcctcgt gatgggcggc gccatcgacg agatcatccg catcggccac cgcaaccgcg
     3061 acgagggccg tctcgtctcg atccaggcgg tcttcgagcg gcccctcgac aagaccgagc
     3121 ccgctggcta ggataccgat ccatgagcga tgccctgacc ggacagacgg tggagctgct
     3181 gcagcagatg atccgcaacc ggtgcgtgaa cgacggcacc ctgctctccg gccaggagaa
     3241 gcgcaacagc gacacgctgc gcagctatct cgcgggcagc ggcctcgaca tggaggtgta
     3301 cgagccggcg cacgctcccg ggcgggcaag cctcgtggcc cgtatcgagg gcaccgatcc
     3361 caaggcgccc acgctgtgcc tcatgggcca caccgacgtg gtgccggtga accccaagac
     3421 ctggacgcgc gatcccttcg gcggcgagct cgtcgacggc gaggtgtggg gacgcggcgc
     3481 ggtggacatg ctgaacctca ccgcctcgca ggccgtcgcg ctcaagacgc tggccggccg
     3541 cgggtggcgg ccgcgcggca cgctggtgta cctggcctgc gccgacgagg aggccggcgg
     3601 gacgctcggc gccgggcacg tggtcgagcg gcactgggac gcgctccggg ccgattgctg
     3661 cctgaccgag aacggcggca ccgtgagccg cggggcgagg ggcgacgagc tgaacgtcac
     3721 cgtgcacgtg ggtgagaagg gggtggcctg gcgccgcctg cgcgtcaagg gcacgcccgg
     3781 ccacggctcg atgccctacg gcgcgaacaa cgccctgatc aaggcggcgc aggtggtgag
     3841 ccgcctggcc gactaccggc cctcgccgta cgtggacgag ctgtggcggg ccttcgtgga
     3901 cacgctccac ctcgatcccg ccatgaagga agccctggcc gatcccgcgc gcgtggacga
     3961 gtcaatcgaa gggctcgccc cctcgctggc caagctcgcg tggtcggcca cccacaccac
     4021 gttctcgccc aacgtctgcc acggcggcgt gaagacgaac gtgatcccgg acgcggtgga
     4081 cgtcgaggtg gacatccgca ccgtccccgg cgacaacgac gacgaggtgc gccggcatct
     4141 ggacaaggcc ctgggtggcc tggcggagga gatcgaggtc gagaagctct tctccaagcc
     4201 ggcgtcgatg tcgcccaccg gcacccccct ctggcacgtg ctgggccgcg tggtggacgc
     4261 gcactacccg ggcgcgaagc tcctgcccaa gatgatcgtg ggcttcaccg acgcgcccta
     4321 cttccgcgag aagggcgcgg tcgcctacgg cttcgggctc ttctcgcgga cgctgaccgc
     4381 ggaggcgatg tccggccgct tccacggtaa cgacgagcgc atcgacgtcg aatccctggc
     4441 cctcaccacc caggcctggc tcgaggtgtg cgagcagttc ctcgggtaag tgatcccgat
     4501 caccggctat agcgaccggc tctccgccgc gcccggcgag cgcatcgcct tcaaggtctc
     4561 gagcgcggcg acgggaccgt accgcgccag cctcgcgcgg gtcatccacg ccgacccgaa
     4621 tccggccggg cccggcctga agatcgagga cctcgccgcc cggttcacga tcgagcggcc
     4681 ctcccgcgcg cagccgctcg cccagggctc ccacgtgcgc gtggacgccg ccgcggccgt
     4741 gcacacgccg gggccgctca ccgtgaccgc tctcgtctgg ccgagcttcg cggccaagcg
     4801 cgagcagtgc gtgatctcgc gctgggacga gaccggtcag gcgggctggg ccctgtcgct
     4861 gacgtccggc ggggtgaccg cgcgggtcgg cgctccgggc ggcgcgccgg tcaccgtcac
     4921 caccggccag gcccttccgc tccggcggtg gcaccggatc tggctcgtgg ccgatgcggc
     4981 cgcgggcacg ctgcgcgtcg gccacgcccc gctcaccggc gggggccggg aagaggcgtc
     5041 ggcgcccctg ccgcccgcgg cccggctcga cgcggccgcg ccgctgctga tcggcgcgcg
     5101 gctcgcgcgc gaggcgcgcg agcactacaa cggcaagatc gaggacccca tgctgcttgc
     5161 cgccgccgcg gccgcgcccg agacgctgct gctcgatccc ctgctgccgc ccgatggcct
     5221 gatcgcgggc tgggacttct cgcagggcat cgccgggctc gacgtcaccg acgtcgggcc
     5281 gcaccgcctc ggcggccggc tcgtgaacct gcccacgcgc gcggtgaccg gggcgcgctg
     5341 gacgggacgc gagatgtgct ggcgccacgc cccgcgcgag tacgcggcca tccacttcca
     5401 cgacgacgac gtgtacgacg cgggctgggc caccgacttc gagttcacgg tgccggacga
     5461 cctgccgagc ggcgcctatc tcatgcggct gtccgcggag ggccacgccg acgacctgcc
     5521 gttctacgtg cggccggcgc gcggccggcc gcgcgcggac gtgctctaca tcgcgtccac
     5581 ctacacctac caggcctatg cgaatcatgc acgcggctca tccgacgcgg ggtaccgcga
     5641 gcgcgtcgcc gcgtggggcg cctatcccca cgccccggac aaccatccgg actacggctg
     5701 gtccacctac aaccggcacc gcgacggcag cggcatctgc tactcgtccc gccttcgccc
     5761 ggtgctgacg ttccggccgc gctacctcac cttcctcgac gcgcgcggct cgggcctgcg
     5821 ccactactcc gcggacacgc acctgctcga ctggctcgag gcgcagggga tccggtacga
     5881 cgtcgtcacc gacgaggacg tggaggccga gggcgcggcc ctgctcgccc cgtacgcgac
     5941 cgtgctcacc ggctcgcacc ccgagtacca caccgcgcgc acgctcgacg cccacgcggg
     6001 ctacctggac ggcggcggca agctcgccta cctgggcggc aacggcttct actggcgcat
     6061 cgccaccagc cgcgcggtgc ccggcgtcat cgaggtgcgc cgcgccgagg gcggcatccg
     6121 ggcctgggac gcccagaccg gcgagtacta tcacgccctc gacggtgcct acggcgggct
     6181 ctggcgccgc aacggccgcc caccgcagcg gatcggcgcc gtcggcttct ccgcgcaggg
     6241 cctcttcgag ggctcgtact atcgccgcca gccgggcgcg gacgacccga aggcagcctg
     6301 gatcctcgac ggcgtcgagg acgcggtgat cggcgacttc ggcctctcgg ggggcggggc
     6361 ggccggcttc gagctcgacc gtgcggaccc cgcgctcggc acgcccgcgg gcgccatcgt
     6421 ggtggccagc tccgaggggc acggccccag cttcatcgtg gtgcccgagg agctgctctc
     6481 gcacctggcg acggtcagcg gcgagaaacc ggcccggctg ctgcgggccg acatgaccta
     6541 tgccgagctg ccgggcgggg gcgcgatctt cgcggtgggc tccatcacct tctgcggcag
     6601 cctgtcgcac aatcgctacg acaacaacgt ctcccgtatc cttcgcaatg tcctggaccg
     6661 cttccgcgcc cgtcgatgaa gctgacggat gcgcttcaga tgacggccga cgaggtcgtc
     6721 gcgcttgtcg gcggcggcgg aaagaccacg gcgatgtttc gcctggcgcg cgaggtggtg
     6781 gacggcggcg ggcgcgcgat caccaccacg accacccgga tcttcggcgc gcagatcgcg
     6841 ctggcgcccg cccacgtccc cgccgtcgac ctcacgcggg agcgcgtcgc ggccgcgctg
     6901 gcggcccacc ggcacgtgct cgtcatcggc gagaccgacg cgggcagcgg caaggccgag
     6961 ggcgtctcgc tcgatctgtt ccggcgcctg cgcgcctggt tcccagcggt ctgcctcctc
     7021 aacgaggccg acggctctcg catgcgcccg ttcaaggccc ctgccgatta cgagccggtg
     7081 atcccgaccg agacgacgct ggtcgttccc gtggtcggcg ccgacgtctt cggcaagccc
     7141 ctggacgccg atcacgtcca ccggccggag ctcgtcggcg cgctctgcgg cgcgccgctg
     7201 gggacgccga tcacgccctc gatcgtggcc cgggtcctgg cccatccccg gggcggacgg
     7261 aagggcgtgc cggccggcgc ccgggtcgtc gttctgatca acaaggtgga agcgctcccg
     7321 gatcacgcgg cggcgcgcga gaccgccgcg tgcctcctgc gcgagccggc gatccattcc
     7381 gtcgtgctgg ccaacctgcg cgcggaggac ccggtgctcg aggtctgcgc gcggtgacgc
     7441 tcgacgtgcc gcacctccga gccgacacgc cgggcgttgc caacgtcctg cacttcaaca
     7501 acgcgggcgc tgccctgcca ccgcgaccgg tcctggcggc cgtgaccggc catctggagc
     7561 gagaggcggc catcggcgga tacgaagccg ccgccgaggc ggcggaccgg atcgccgacg
     7621 tctatgacgc cgtcgccgcc ctgatcggcg cgggccctga cgagatcgcg ttcgtggaga
     7681 acgcgacccg ggcctgggac atggccttct acgccatccc cttccgggcc ggagaccgca
     7741 tcgtcaccgc gcgggccgag tacgcgtcca actatctcgc gttcctccag atgaagcggc
     7801 gcgcgggtgt cgagatcgac gtggtggacg acgacgcctc cggacagctc gacgtggccg
     7861 cgctcgagcg cgcgatccgc ccgcgcaccc ggctcatcgc gatcacccac gtccccaccc
     7921 agggcgggct ggtgaacccg gccgcggagg tgggctggat cgccgcgcgg cacggcatcc
     7981 tctacctgct cgacgcctgt cagtcggtcg gccagctcac cgtcgacgtc gcccgaatcg
     8041 gctgccacat gctctcggcc accgggcgca agtacctgcg tggcccgcgc ggcaccggct
     8101 tcctgtacgt gcgacgcgac accatcggcg cgctggagcc gcccttcatc gatctcgagg
     8161 ccgcgtcctg gatcgacgcc gatacctaca cgctccgcga cgacgcccgg cgcttcgaga
     8221 actgggagcg gttcgtggcc ggccagatcg gcctcggcgt ggcggtgcgc tacgcgctgg
     8281 ccctgggcat cgaggccatc gagccgcggg tcaaggcgct cgcagacctg ctccgccgcg
     8341 agctggcgaa gcggcccgga gtgaccgtgc acgacctggg gaccgagcga tgcggcatcg
     8401 tgaccttcct caaggccggc gaggcgccgg acgccacccg gcggcggctc cgggccctgg
     8461 acatcaacgt cacccacacc caggcccgct cctcgcggct cgacctgccc gcgcgcggct
     8521 tcgacgcgct cgtccgcgcc agcgtccact actacaacga cgaggccgag gtcgagcgct
     8581 tcgtgcgcgc ggtctcggca tcctagaaga ggaggacccg catgaccacg gtggaaacag
     8641 ccctcgggcc ggtggccacg gccgagctcg gcgccacgct gatgcacgag cacatcgtca
     8701 cccggtcgcc gggggtgcag gagaactggc cgcacctctg ggaccgcgca ggcatcctcg
     8761 cgatggcgga gcggaagatg gccgatctcc acgcccgcgg catccgtacg atcgtggacc
     8821 tgaccaccgt ggacctgggc cgcgacatcg gcctgatcct ggacgtggct cggcgctccc
     8881 gtgtccacgt gatcgtggcc accggcgtct ggtggatgcc ccagcggtac ttcagcggcc
     8941 acgaggtgga cgcggtggcc gatctgttcg tccgcgacat cacgcagggc atcggggaca
     9001 gcggcgtcaa ggccgccatc atcaagtgcg ccaccgatac cgcgggggtg accccggtca
     9061 tcgagaacat cctccgcgcg gccgcgcggg cccagaaggc gaccggggtg ccgatctcga
     9121 cccacacctg ggccgcgggc cggaccgggg aggcgcagca ggcgatcttc gcgcaggagg
     9181 gcgtggatct ccgtcgcgtc atcatcggcc acagcggcga cagcgaggac ctggggtacc
     9241 tgcgcgggct catggaccgc ggcagcacca tcggcatgga ccgcttcggc ctcgagcact
     9301 tcctgccgac gccgaaacgg gtcgaggtga tcgcccgcct gtgcgcggaa ggctacgcgg
     9361 cgcgcatggt cctctcgcac gacgccaact gctggagcga catgctctcc gaggacgaca
     9421 agcggcggac ccggccgcgg tggaactaca accacatctc cgacgacatc ctgcccgcgc
     9481 tccgcaaggc gggggtcccc
//
LOCUS       DASUFA010000176         8653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_958241, whole genome shotgun sequence.
ACCESSION   DASUFA010000176 DASUFA010000000
VERSION     DASUFA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958241"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..769
                     /locus_tag="VFX87_06265"
     CDS             122..769
                     /locus_tag="VFX87_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4994552.1"
                     /translation="MSGANGATEFKDVVGSLDELRTLMGEPSEIALRKDIGRLDEHCR
                     AFIARAPFLLVATADTKGRCDVSPKGDAPGFVLVVDDRTLLIPDRPGNKRFDGMRNLL
                     ENPGIGLIFLLPGSEETLRVNGRARIVRDPEWLARLAAQGKTPRLAIAVDVEEAFLHC
                     AKCVKRSGLWEPARWPDREGLASPAQMFRDHARLNTMTVEELDQRLQDGYRKNLY"
     gene            787..1629
                     /locus_tag="VFX87_06270"
     CDS             787..1629
                     /locus_tag="VFX87_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4994553.1"
                     /translation="MATMPIEVRPITGALGAEIHGVNLGADLDEASVAAIRRALLDHC
                     VVFFRDQRFDATRHKALARRFGEIFVHPNYASVVPDPEIIEIRREPGDRRIVGEEWHT
                     DTTMMAEPPMGAILYAVDVPPYGGDTLFASQYLAYEALSDGMKRLLEGLRAVHSDRKV
                     AGPQAGLNAYRATKVREEGWEETVSTHPVVRTHPETGRKLLFVNHSYTIRFDGMTEAE
                     SAPLLGFLLEHGHRPEFTCRFRWANGSVAFWDNRCVKHLAVHDAGAFRRIMRRVQIAG
                     DKPV"
     gene            1633..2448
                     /locus_tag="VFX87_06275"
     CDS             1633..2448
                     /locus_tag="VFX87_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994554.1"
                     /translation="MLIVDAQVHIWGSGTPLPPHRPTAVFSKDELLAEMDAAGVQAAL
                     IHPPGWDPGSDRLALEAAREHPERLAVLGKFPLDRAESRALMDGWTRQPGMLGLRFVF
                     LQPHQKSWPTDGTLDWLWPAAERAGAPLALLAADFLPLVGQVAERHPGLKLIVDHLGR
                     PHRTKDEAAWANLSELLALAKHPNVAVKATGAPSYSSEPYPYRNLHPHLRRLHEAFGP
                     ERLFWGTDITRMSCTWRQCVTLFTEELPWLTTRDKELIMGRALCAWLDWKLPG"
     gene            2456..3493
                     /locus_tag="VFX87_06280"
     CDS             2456..3493
                     /locus_tag="VFX87_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEX4994555.1"
                     /translation="MKIVVTARTGAREFECDPGEKILHAGLRSGVELPYECGTGTCGT
                     CKARLVSGRVESGWPEAPGIRYLKDPAELLMCQCVAREDCALEVGVLKTREAAAPLPG
                     ALGGRVRGWRRLTHDVAAFDVDLDRPLDFDAGQFALLTVPGVIGARAYSMVSFERAAR
                     RLSFVVKKKPGGAVSEWLFGAAVEGTRLGLFAPLGHATFQPGLPKHVLCIAGGSGIAG
                     MMSILQRAGEAGHFAGWDGHVFFGVRAGRDGFFLDELQALQARSPARLAVTVALSDED
                     VPASLPAAYPGLAFARGLVHAVAGERMKGRFADVRAYVAGPPPMVDASLRLLLREARL
                     SPADIRYDKFS"
     gene            3511..4212
                     /locus_tag="VFX87_06285"
     CDS             3511..4212
                     /locus_tag="VFX87_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX4994556.1"
                     /translation="MGQYEQHVFVCTSGETCPEQGDTEKFVKILRERAKQVGRPSDVR
                     INKAGCFSQCGRGPMIVFYPDDVWYAGVQESDLDEIFTSHIVGGRPVERLRYNPGKPG
                     ANKVSEAELAEIKSAYAARAQAPAPDGPAPAWKRVGPAADVPANGMKEFSVGGVNVLI
                     VHTGAEYVALQALCPHETIPLEQGIHDGSVLTCLEHMWQFDIRTGAPLGDALEGLRRY
                     HLKADGGELYVALEA"
     gene            4285..4476
                     /locus_tag="VFX87_06290"
     CDS             4285..4476
                     /locus_tag="VFX87_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994557.1"
                     /translation="MRNLLTTALGVLLGTLALTACSGVPVPPAYTQEELAARCARTGG
                     WWKESRSYSLVSGHCEYRD"
     gene            4419..4778
                     /locus_tag="VFX87_06295"
     CDS             4419..4778
                     /locus_tag="VFX87_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994558.1"
                     /translation="MEGVAVVLAGQRPLRVPRLIASRTRIRALLALLGGLLAVAAVAR
                     AESPWTLWERPVDLNSPAKGDWRRTQVFEAERWCKGAMTTAINRNLLAGWKGGRLDPK
                     AKITEYQCLPEGELPRR"
     gene            complement(4732..5592)
                     /locus_tag="VFX87_06300"
     CDS             complement(4732..5592)
                     /locus_tag="VFX87_06300"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007255979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEX4994559.1"
                     /translation="MPLPADGPVTAGDLLTLEALGHFRRTSTVHGIGLVLHAWGVIAG
                     AIALYLRWPSVLTLGVATLVIAARQLGLMVLMHEAAHWLLFRQGRLNTWAGTWLCAAP
                     LPVSRGRLALALAGDLGGWTALRRIAGWRPLRDGFAETWRRARAPLLANAALCAVLTA
                     LGGWPLYLLTWVLPWATWFQLVTRVRDIAEHGLVHDAEDPLRNTRTIGAGLLARAFVA
                     PYGVNYHLEHHLLVFVPCWKLREVQALLLAKGLGGRMERAASYGEVLGRATCGIIGAG
                     ARPRAGTDIP"
     gene            5649..6053
                     /locus_tag="VFX87_06305"
     CDS             5649..6053
                     /locus_tag="VFX87_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4994560.1"
                     /translation="MTTTSTATGPSIDLLMEINAAFNSRDVDRILSFFAEDATFLMAR
                     GPEPCGRRVHGKAAIGKVLADRFKVISGMRWDHVDHFVTANRAVSVWMVTGKGADGEL
                     LNYQGCDIYEFRGDKILNKDTYWKRVEPDGRL"
     gene            complement(6050..6439)
                     /locus_tag="VFX87_06310"
     CDS             complement(6050..6439)
                     /locus_tag="VFX87_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994561.1"
                     /translation="MKRALLMFARALGRRCPSCGAGPVVTRWIHTVAACPRCRLRLDR
                     GEPDYFLGAIVFNMAFAEGLFAAALLGVLLCTWPNPPWDALYYGGITGLIVAPILFYP
                     YSKLLWLAFDLLFRPLRPEDFEPPVVS"
     gene            complement(6440..6925)
                     /locus_tag="VFX87_06315"
     CDS             complement(6440..6925)
                     /locus_tag="VFX87_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4994562.1"
                     /translation="MTYEPSDKIGTLTDAELATFLDEPWNGRIATITPEGWPYVAPVW
                     YEFERATRSFLVVGRERAEWIAHIRTNPRVAFHVADDQHAHHTRVQVQATARILEGPI
                     APTASPTLLDLTHRLSRRYLGPDGPRYAEKTLGRPRVLVRLDPVRWTSWTGQEWHPRY
                     R"
     gene            complement(6928..7764)
                     /locus_tag="VFX87_06320"
     CDS             complement(6928..7764)
                     /locus_tag="VFX87_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994563.1"
                     /translation="MAEGRAARWLVRRPLLAGAGLTLAGLVVAGALAEVVAPYSPTTV
                     DASHMFSRPGGGHLFGTDRFGRDLLSRVVFGIRVSLGIAGAAIAIALLAGGTLGMVAG
                     LGRAADQALGRVMDVFFAFPPILLAIGIAAVLGAGPGTAVIAIAVVYAPLFFRVVRGS
                     VLAESAQAYVEAAEALGRGRLGILFRHVLPNIISPIVIQTAVCLSYGILIESALSYLG
                     VGVQPPTPSWGAILNEGKEFLMLAPWVSLFPGAAIMLAVLSLNVLGDGLRDALDPRAE
                     MR"
     gene            complement(7757..>8653)
                     /locus_tag="VFX87_06325"
     CDS             complement(7757..>8653)
                     /locus_tag="VFX87_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994564.1"
                     /translation="VSLLVFVMVRLIPGTVVEQLLGQAAMASPEVVASFRRFFGLDQP
                     WHVQYGVWLAGLLRGDLGVSWLSGRPVLALFLERLPVSAELAVLSVGWSLLLGIPLGT
                     ASAVWRGGLRDGLIRVGATLGLSMPAFWQGAVLILFFSLYLGWMPALTWVPFTRSPSA
                     NLAIMALPALTLGTATAAMITRMARSSMLDVLGREYVRTARAKGVAERRVTFHHALRN
                     ALIPVVTVAGVQLGYIVGGIVVVEDVFTLPGVGRLLLDAIFQRDYPVVQGVILLLAAA
                     FMALNLLVDLLYTVIDPRLRRG"
BASE COUNT         1368 a         3038 c         3165 g         1082 t
ORIGIN      
        1 gatccggcgg gccgcgtgaa gtgcgcccgg ttcggcgaga actactccga gcggatcgag
       61 acctgggagg gacggtgatg gtatgttcgg gtgcacgaga gatcttcccg ggaggcgacg
      121 catgagcgga gcgaatgggg cgacggagtt caaggacgtg gtgggcagcc tcgacgagct
      181 gcgcacgctg atgggggagc cctccgagat cgccctgcgc aaggacatcg gccggctcga
      241 cgagcattgc cgggccttca tcgcgcgagc gcccttcctg ctcgtggcca ccgcggacac
      301 caagggccgc tgcgacgtga gccccaaggg cgacgccccc ggcttcgtcc tggtggtgga
      361 cgaccggacc ctgctgatcc cggaccgtcc aggcaacaag cgcttcgacg gcatgcggaa
      421 cctgctcgag aacccgggca tcggcctcat cttcctgctg ccgggcagcg aggagacgct
      481 gcgggtcaac ggtcgcgccc ggatcgtgcg cgatcccgag tggctcgcgc gcctggccgc
      541 gcagggcaag accccgcggc tcgccatcgc ggtggacgtg gaggaggcct tcctccattg
      601 cgcgaagtgc gtgaagcgct ccggcctctg ggagcccgcg cgctggccgg accgcgaggg
      661 gctggcctcg ccggcccaga tgttccgcga ccacgcccgg ctcaacacca tgacggtcga
      721 ggagctcgac cagcgcctgc aggacggcta ccggaagaac ctgtactgat ccccaggaga
      781 aaagtcatgg ccacgatgcc gatcgaggtc cgtcccatca ccggtgccct gggggccgag
      841 attcatggcg tgaacctggg cgccgatctc gacgaggcga gcgtggccgc catccggcgc
      901 gccctcctcg atcactgcgt cgtgttcttc cgcgaccagc ggttcgacgc cacccggcac
      961 aaggccctcg cgcgccgctt cggcgagatc ttcgtgcatc ccaactacgc gagcgtagtc
     1021 cccgacccgg agatcatcga gatccgccgc gagcccggag accggcgcat cgtgggggag
     1081 gagtggcaca cggacaccac catgatggcg gagcccccga tgggagccat cctctacgcc
     1141 gtcgacgtgc ctccctacgg cggcgatacg ctcttcgcca gccagtacct cgcctacgag
     1201 gcgctgtccg acggcatgaa gcgcctgctg gagggcctgc gcgccgtcca cagcgatcgg
     1261 aaggtggcgg gcccccaggc gggattgaac gcgtaccggg cgaccaaggt gcgcgaggaa
     1321 ggatgggagg agacggtgag cacccacccc gtcgtgcgca cgcatccgga gaccgggcgc
     1381 aagctgctct tcgtcaacca ctcctacacc atccgcttcg acggcatgac cgaagccgag
     1441 agcgcgccgc tgctcggctt cctgctcgag cacggccacc ggcccgagtt cacctgccgt
     1501 ttccggtggg ccaacgggtc ggtcgctttc tgggacaacc gctgcgtcaa gcatctggcg
     1561 gtgcacgacg ccggggcgtt ccgccggatc atgcgccggg tccagatcgc gggcgacaag
     1621 ccggtctagg ccgtgctgat cgtcgacgcc caggtgcaca tctggggcag tggcacgccc
     1681 ctgccgcccc accgcccgac cgcggtgttc tcgaaggatg agctgctggc ggagatggac
     1741 gcggcgggcg tccaggcggc gctcatccac ccgccgggtt gggacccggg ctcggaccgg
     1801 ctcgcgctcg aagcggcccg cgagcaccca gagcggctgg cggtcctcgg gaagtttccg
     1861 ctcgaccggg cggagagtcg cgccctcatg gacggctgga cgcgccagcc gggcatgctg
     1921 gggctgcggt tcgtcttcct gcaaccacac cagaagagct ggccgacgga cggcaccctg
     1981 gactggctgt ggccggccgc cgagcgcgcg ggagcgccgc tggcgctgct cgcggcggac
     2041 ttcctgccgc tcgtcgggca ggtggccgag cgccacccgg ggctcaagct gatcgtggac
     2101 catctcggcc gcccgcaccg caccaaggac gaggcggcct gggccaacct gtcggagctc
     2161 ctggccctcg cgaagcaccc gaacgtggcg gtcaaggcga cgggggcgcc cagctactcg
     2221 agcgagccct atccctaccg caacctccat ccgcacctcc gccggctcca cgaggccttc
     2281 ggcccggagc gcctgttctg ggggacggac atcacgcgca tgtcgtgcac ctggcggcag
     2341 tgcgtgacgc tgttcaccga ggagctgccg tggctcacga cgcgcgacaa ggagctgatc
     2401 atgggccgcg cgctctgcgc ctggctcgac tggaagctgc cggggtgagc cgggcgtgaa
     2461 gatcgtcgtc accgcccgga ccggggcgcg cgagttcgag tgcgaccccg gcgaaaagat
     2521 cctccacgcg gggctccgga gcggcgtcga gctgccgtac gagtgcggca ccggcacctg
     2581 cggcacgtgc aaggccaggc tggtgagcgg ccgggtggag agcgggtggc ccgaggcccc
     2641 ggggatccgc tatctcaagg acccggccga gctgctcatg tgccagtgcg tggcgcgcga
     2701 ggactgcgcg ctcgaggtcg gcgtgctcaa gacgcgcgag gcggccgcgc cgctccccgg
     2761 cgcgctgggc ggccgcgtgc gcggctggcg ccgcctgacc cacgacgtgg ccgcgttcga
     2821 cgtggatctc gaccgcccgc tcgacttcga cgcgggccag ttcgcgctcc tcaccgtccc
     2881 cggcgtcatc ggcgctcgcg cctactccat ggtgagcttc gagcgggcgg cccggcgcct
     2941 gtcgttcgtg gtgaagaaga agcccggcgg ggcggtgagc gagtggctgt tcggcgccgc
     3001 ggtcgagggc acgcgcctcg gcctcttcgc gcccctcggg cacgcgacct tccagcccgg
     3061 gctcccgaag cacgtgctgt gcatcgcggg gggcagcggg atcgccggca tgatgtcgat
     3121 cctccagcgc gccggcgagg caggccattt cgcggggtgg gacggccacg tcttcttcgg
     3181 ggtgcgcgcc gggcgcgacg gcttcttcct ggacgagctc caggcgctcc aggcacgctc
     3241 cccggcccgg ctggccgtca ccgtcgcgct gtcggacgag gacgtgccgg cgtcgctgcc
     3301 cgcggcctat ccggggctcg cgttcgcgcg cggattggtc cacgcggtgg ccggcgagcg
     3361 catgaagggc cggttcgcgg acgtccgcgc ctacgtggcg ggcccgcccc cgatggtgga
     3421 cgcgagcttg cgtttgctgc tgcgcgaggc gagactgtcg cccgccgaca tccggtacga
     3481 caagttcagc taggaccgaa aggagcgacg atgggccagt acgagcagca cgtcttcgtg
     3541 tgcacgagcg gcgagacctg cccggagcag ggcgacaccg agaagttcgt gaagatcctc
     3601 cgggagcggg cgaagcaggt cggtcggccg agcgacgtgc gcatcaacaa ggccggctgc
     3661 ttctcgcagt gcgggcgcgg cccgatgatc gtcttctacc cggacgacgt ctggtacgcg
     3721 ggagtccagg agtcggacct cgacgagatc ttcacctccc acatcgtggg cggccggccg
     3781 gtcgagcggc tgcgctacaa ccctgggaag ccgggcgcga acaaggtgag cgaggccgag
     3841 ctggccgaga tcaagagcgc ctacgccgcc cgcgcccagg cgcccgcgcc ggacgggccc
     3901 gcgcccgcgt ggaagcgcgt ggggcccgcc gccgacgtcc ccgccaacgg gatgaaggag
     3961 ttctcggtcg ggggcgtcaa cgtcctcatc gtccacaccg gcgccgagta cgtggccctc
     4021 caggcgctgt gcccgcacga gacgatcccg ctggagcagg gcatccacga cggctcggtc
     4081 ctgacctgcc tcgagcacat gtggcagttc gacatccgga ccggggcgcc gctgggcgac
     4141 gccctcgaag gcctgcgccg ctaccacctc aaggccgacg gcggcgagct gtacgtcgcg
     4201 ctggaggcct gagcagcggc ggagctttgg ccgacgcggc cgaccgcgtc gccgaagcgg
     4261 tggggtcgta gtatggtggc ggatatgcgc aatctgctga cgaccgcgct gggcgtactg
     4321 ctcggcacgc tggcgctgac ggcctgctcc ggcgtcccgg tgccgccggc ctacacccag
     4381 gaggagctcg cggcccggtg cgcccgcacg gggggctggt ggaaggagtc gcggtcgtac
     4441 tcgctggtca gcggccactg cgagtaccgc gactgatcgc ctcccggacc cggatccgcg
     4501 ccctgctcgc cctgctcggc ggcctgctcg ccgtcgccgc ggtcgcccgc gccgagagtc
     4561 cgtggacgtt gtgggagcgc ccggtcgatc tcaactcccc cgccaagggc gactggcggc
     4621 gcactcaggt gttcgaggcc gagcgctggt gcaagggagc catgaccacg gccatcaatc
     4681 ggaacctgct cgccggatgg aagggtggcc ggctcgaccc caaggcgaag atcacggaat
     4741 atcagtgcct gcccgagggc gagctcccgc gccgatgatg ccgcacgtcg cgcgtccgag
     4801 aacctccccg tagctcgccg ctcgctccat ccgccctccg aggcccttcg cgagcagcag
     4861 cgcctggacc tcgcgcagct tccagcacgg cacgaacacg agcaggtgat gctcgaggtg
     4921 gtagttcacc ccgtagggcg caacgaaggc ccgcgcgagc aggcccgcgc cgatcgtgcg
     4981 cgtgttccgg agcgggtcct ccgcgtcgtg gaccagcccg tgctcggcga tatcgcggac
     5041 ccgcgtgacg agctggaacc acgtggccca gggcagcacc catgtgagca ggtagagcgg
     5101 ccagccaccg agggcggtga gcacagcgca gagcgccgcg ttcgcgagga gaggagcccg
     5161 ggcccgccgc caggtctcgg cgaagccgtc acgcaggggc cgccagcccg cgatccgcct
     5221 gagcgccgtc cacccgccga ggtcgcccgc gagcgcgagg gccaggcgtc cgcgcgagac
     5281 cggcagcggc gccgcgcaga gccaggtccc ggcccaggtg ttcaggcgcc cctggcggaa
     5341 gagcagccag tgcgcggcct cgtgcatcag caccatgagc ccgagctggc gggcggcgat
     5401 gaccagggtg gccacaccca gcgtgagcac cgacggccag cgcaggtaga gcgcgatcgc
     5461 gcctgcgatg acgccccacg cgtgcaggac caggccgatg ccgtgaacgg tcgaggtgcg
     5521 ccggaagtgc cccagcgcct ccagcgtcag caggtcaccg gccgtgaccg ggccgtcggc
     5581 gggcaggggc atgcgcggat gataccaccc gcttgacggg cgggcgcgac gcgggcagag
     5641 tcgggcccat gacgaccacc tcgaccgcca cgggccccag catcgacctg ctgatggaga
     5701 tcaacgccgc cttcaacagc cgcgacgtgg accggatcct gagcttcttc gcggaggacg
     5761 cgaccttcct gatggcccgc ggccccgagc cgtgcgggcg ccgcgtccac ggcaaggcgg
     5821 cgatcgggaa ggtcctggcc gaccggttca aggtcatcag cggcatgcgc tgggaccacg
     5881 tcgatcactt cgtgaccgcc aaccgcgcgg tctcggtgtg gatggtgacg ggcaagggcg
     5941 cggacggcga gctgctcaac taccaggggt gcgacatcta cgaattccgc ggcgacaaga
     6001 tcctcaacaa ggacacctac tggaagcgag tcgagccgga cggccggctc tagctcacga
     6061 ccggcggctc gaagtcttcc ggcctgagcg ggcggaagag caggtcgaag gcaagccaca
     6121 ggagcttgga gtagggatag aagaggatcg gcgccacgat cagcccggtg atgccgccgt
     6181 agtagagggc gtcccacggc gggttcggcc aggtgcagag caggactccc agtagagcgg
     6241 cggcgaacag gccctccgcg aacgccatgt tgaagacgat cgcgcccagg aagtagtccg
     6301 gctcgccccg gtcgaggcgc aggcggcacc gcgggcaggc cgccaccgtg tggatccacc
     6361 gcgtgaccac cgggccggcg ccgcagctgg ggcagcggcg cccgagggcg cgggcgaaca
     6421 tgaggagcgc gcgcttcatt cagcggtagc gcgggtgcca ctcctgaccg gtccaggagg
     6481 tccagcgcac cggatcgagg cgcaccagca cgcggggacg gccgagggtc ttctccgcgt
     6541 agcgcgggcc gtccgggccc aggtagcgcc gcgagaggcg atgcgtcagg tcgagcaggg
     6601 tcgggctcgc ggtgggggcg atcgggcctt ccaggatgcg cgcggtggcc tgcacctgga
     6661 cgcgcgtgtg atgggcgtgc tggtcgtcgg ccacgtggaa ggccacgcgc ggattcgtgc
     6721 ggatgtgggc gatccactcc gcccgctcgc gcccgaccac caggaagctg cgggtggcgc
     6781 gctcgaactc gtaccagacc ggggccacgt acggccagcc ctcgggggtg atggtggcga
     6841 tgcggccgtt ccacggctcg tccaggaacg tggccagctc ggcgtcggtc agggttccga
     6901 tcttgtcgct cggctcgtag gtcatgctca cctcatctcg gctcggggat cgagggcgtc
     6961 gcgcaatccg tcgcccagca cgttcaggga cagcaccgcc agcatgatgg ccgcgcccgg
     7021 gaagagggag acccacgggg cgagcatcag gaactccttg ccctcgttca ggatcgcgcc
     7081 ccacgacggc gtcggcggct gcacgcccac ccccaggtac gagagcgccg actcgatcag
     7141 gatgccgtag gacaggcaca ccgcggtctg gatcacgatg ggcgagatga tgttcggcag
     7201 cacgtggcgg aacaaaatgc cgaggcggcc gcggcccagg gcctcggcgg cctccacgta
     7261 ggcctgggcc gactcggcca gcacgctgcc gcggacgacg cggaagaaga gcggcgcgta
     7321 gacgaccgcg atcgcgatca ccgcggtgcc gggcccggcg cccagcaccg ccgcgatgcc
     7381 gatggccagc aggatcggcg ggaaggcgaa gaagacgtcc atcacgcggc ccagcgcctg
     7441 gtccgcggcg cggccgagac cggccaccat ccccaacgtg ccgccggcca gcagcgcgat
     7501 cgcgatggcc gcgccggcga tgcccagcga gacgcggatg ccgaagacca cgcgcgagag
     7561 caggtcgcgg ccgaagcgat cggtgccgaa gaggtgcccg ccgcccggcc ggctgaacat
     7621 gtggctcgcg tccaccgtgg tgggggagta gggcgcgacc acctcggcga gcgcgcccgc
     7681 caccacgagc ccggcaagcg tgagcccggc gccggccagg aggggccgtc ggaccagcca
     7741 ccgcgcggct cggccctcag ccacgacgca atctcggatc gatcacggtg tacaggagat
     7801 ccacgaggag attcagcgcc atgaacgcgg cggcgaggag cagaatcacg ccctggacca
     7861 ccgggtagtc ccgctggaag atggcgtcga gcagcaggcg gccgaccccc ggcagggtga
     7921 agacgtcctc caccaccacg atgccgccca cgatgtagcc gagctgcacg cccgccaccg
     7981 tgaccaccgg aatcagcgcg ttccggagcg cgtggtggaa ggtcacgcgg cgctccgcca
     8041 cgcccttggc ccgggcggtg cggacgtact cgcgccccag cacgtcgagc atggacgagc
     8101 gcgccatccg ggtgatcatg gcggcggtgg cggtgccgag cgtgagcgcg ggcagagcca
     8161 tgatggccag gttggccgac gggctccggg tgaagggcac ccacgtgagc gcgggcatcc
     8221 agcccaggta gagcgagaag aacaggatca gcaccgcgcc ctgccagaag gcgggcatgg
     8281 agaggccgag cgtcgcgccc acgcggatga ggccgtcgcg gagcccgccg cgccagaccg
     8341 cggaggcggt gccgagcggg atgccgagca gcagcgacca gcccaccgag agcacggcca
     8401 gctccgccga gacgggaagc cgctcgagga agagcgccag caccggacgc ccggagagcc
     8461 acgagacgcc cagatcgccg cgcagcaacc cggcgagcca gacgccgtac tgcacgtgcc
     8521 agggctggtc caggccgaag aaccggcgga acgacgcgac cacctcgggc gaggccatcg
     8581 cggcctgccc caggagctgc tccaccaccg tgcccgggat cagccgcacc atcacgaaga
     8641 ccagcaggct cac
//
LOCUS       DASUFA010000177         6768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1409211, whole genome shotgun sequence.
ACCESSION   DASUFA010000177 DASUFA010000000
VERSION     DASUFA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409211"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1132)
                     /locus_tag="VFX87_06330"
     CDS             complement(191..1132)
                     /locus_tag="VFX87_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4994565.1"
                     /translation="MAPLTPVDRVRVTTVVDNYIDALRQDEKVAHRHSAFVARQMVDL
                     RAEHGLAHFVEITRGAETVRIAFDFGPSEAAITHNFRVLGLDPGAIDTLALSHGHWDH
                     FGGLLGFLRTYRPAMKKPLTFHGGADHFLTRWNQRGDDRVSMGRLYREEIERYDVQVE
                     TVRTPTLLAEGVLLSGEMHEAEPFEPIPANLRVERDGAIVPDDFMGEQTLIAHVKGRG
                     LVVVTSCSHRGIVGICRNATRIAGVSKVHAVIGGFHLSGLKEDRVTRVVDAFRALEVD
                     WVVPQHCTGLESMATLLHRLPTETVVTSVGSTFTFQA"
     gene            complement(1194..2264)
                     /locus_tag="VFX87_06335"
     CDS             complement(1194..2264)
                     /locus_tag="VFX87_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4994566.1"
                     /translation="MTLSTRDAQLPAAYSDRLEALSLAPLWTALHTLLPPERQTRVVP
                     HRWRWSELRAPLLEAARLVPIEQAERRVLVLCNPGLPGNFSATATLYAGLQIILPGEA
                     APSHHHTAAALRLIVEGAGAYTTVDGVKCAMEPGDFIITPPMRWHDHGHEGREPVIWL
                     DGLDIPLVRNVEANWASAMRPASTPATETDSSQDEFTAAGLVPRRSRYEETGYPQVRW
                     PWRTARAALAAMAATAPRRAPVVLRHVNPRTGEYPLRTMGSEARWLRPGEETRAERRT
                     ASEVVHVIEGQGESRVGDRTLDWQAGDVFVVPPWHWTAHRNRSGQAPACLFHLNDEPA
                     LRALGLWHEQVRGAAEAEPSEY"
     gene            complement(2284..3516)
                     /locus_tag="VFX87_06340"
     CDS             complement(2284..3516)
                     /locus_tag="VFX87_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994567.1"
                     /translation="MRTIRMLVAVVGLVVAVGPGPAVAQGPIKIGFLSPISGAIAQAG
                     KDMYSGCELYWQERGMQIAGRKLEVILEDTEGQPATAVTKLRKLIDSDRVNLLAGVIL
                     SNVAYALVPPIEAAGIPTIYPINSADDLTQRKRPTWLVRTGFSASGNMHPFGEYAAKV
                     LKYRKVVTISLDYAFGWETVGGFHKAFEDNGGHIIQKIWVPLNIQDYAPYLAQVKRDA
                     DAVFVLALGRWSVLFSKQYADSNLKGRVPLIAGGTHTDEHVLSQLGDAAIGVISAHHY
                     SAALDTPANRKFRAAFEKAYSRSPSFYSENCYTGARVIEEAVRAVNGRVEDRAALMAA
                     LRRVEITDAPRGPVKIDAYGNPTQNIYVRKVEKVGGVLQNTVIHTYPAVSQFWTYDPQ
                     KFLEQPVYSREFPPCKHC"
     gene            3588..4778
                     /locus_tag="VFX87_06345"
     CDS             3588..4778
                     /locus_tag="VFX87_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX4994568.1"
                     /translation="MSQMRVEFVLTAVVGLGALSIDMFLPSLPTIAAVFGAEAAVAQL
                     TVTLFLLAFAASQLVYGPLSDRFGRRWVLIGGLALYSVAGLACALAPTIGVLIGARVL
                     QALGGGTGPVVARAVIRDLYDRERAARVFSYMGMFQSLNPMLAPIVGGYVHEAFGWRA
                     IFFVLAGAGALFVALMSVGVPETNVRRDPTALRPGQMGRNVAELLTDRSYVAYVLVNT
                     LMFGGQFAFISGSSFVLIDVLGVSPSGFGLCFGSVAVGIMTGTFLSGRFGARIQLDRT
                     ILSGTALGAAAGLILAALAWSGILTVAAVIVPMFVFAVGLGLTLPNGMAGAVGPFPRM
                     AGLAAAVAGFLQMTGSALYSVAVGHFYDGTARPMTAAIALAGVAALVCFWRLRWRRAH
                     GVLA"
     gene            4784..5065
                     /locus_tag="VFX87_06350"
     CDS             4784..5065
                     /locus_tag="VFX87_06350"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX4994569.1"
                     /translation="MAARRAKIIRFPTPPPKAPARAATELVEARRCRDQTEALVVRGL
                     LESEGIAVVPRSRLAPSVYPFSVGQQAEVVLLVAAADLPRALAVLARQA"
     gene            complement(5134..5913)
                     /locus_tag="VFX87_06355"
     CDS             complement(5134..5913)
                     /locus_tag="VFX87_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4994570.1"
                     /translation="MAYSDILFEVKNQIAWLTINRPDKGNTFRRQTVLEMIQALNGAR
                     NDAGVRVVVITGAGDRFFCIGGEKEPNDGLLHYHNVPPVVDLYTLIDMMPVPIIAMVN
                     GFSVGGGNVLANMCDLTVASEQATFRQVGPAMGSYDAGFGTWYLEDSVGRKRAKEIWY
                     LNRKYTAKEAQAMGLVNEVAPHAELRARTEAMCEELKKRGPQALAALKAAFHARHNGV
                     TGLSRVTIDHLVANYYHTEEAKEMGRAFNSKQDPDPDKFYK"
     gene            complement(5938..6717)
                     /locus_tag="VFX87_06360"
     CDS             complement(5938..6717)
                     /locus_tag="VFX87_06360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4994571.1"
                     /translation="MPLFDTHAHLHFPELLADLDAVLERARAAGVTGMVTIGTDRETN
                     PAAVALAERLHDVHATVGIHPHDAGEATEPDFEAMERLARGSAKVVALGEMGLDFFRN
                     LSPRDAQETALRRQLDMARRLGKPVVIHCRDAHREALAILSDEHVGEVGGVMHCFSAD
                     VEVAKRCLDLGLMISLAGPVTYKNARALPDVARFVPADRLVVETDCPFLPPHPHRGQR
                     NEPAWVAITAAHVAGLRGVSPEALGEAVTENAHRLFRIPTR"
BASE COUNT          888 a         2389 c         2402 g         1089 t
ORIGIN      
        1 tgcctctcgg tgggcgccgc gcgcgccgac gtggacaccc tcggccgcga ggccgcgagc
       61 ctggtggccc gcgcgatcgc gcgggccgtg cgcgccgcga cggcatccgg cggcctcccc
      121 gcagcgcgcg atctcgacta gagggatccc cgctctcccc tcaggggaga gggcagggtg
      181 aggggttccc ctacgcctga aacgtaaacg tggacccgac cgaggtgacc accgtctcgg
      241 tcgggagccg atggagcagg gtggccatcg actcgagtcc ggtgcagtgc tgcggaacca
      301 cccagtccac ctcgagggcg cggaaggcgt ccacgacccg ggtgacccgg tcctccttga
      361 gtccgctcag gtggaagccg ccgatcaccg cgtgcacctt ggagacgccc gcgatgcgcg
      421 tggcgttgcg gcagatgccg acgatgccgc ggtgcgagca cgacgtgacc accacgaggc
      481 cgcggccctt cacgtgggcg atcagggtct gctcacccat gaaatcgtcc ggcacgatcg
      541 ccccgtcgcg ctcgacccgg agattcgccg gaatgggctc gaagggctcc gcctcgtgca
      601 tctccccgct cagcagcacg ccctcggcga gcagggtcgg cgtccgcacc gtctccacct
      661 ggacgtcgta gcgctcgatc tcctcgcggt agagccggcc catgctgacc cggtcgtccc
      721 cgcgctggtt ccagcgcgtg aggaagtggt ccgccccgcc gtggaacgtg agcggcttct
      781 tcatggcggg gcgataggtc cgcaggaagc cgagcagccc tccgaagtgg tcccagtggc
      841 cgtggctcag cgcgagagtg tcgatggccc cgggatcgag gccgagcacc cggaagttgt
      901 gggtgatcgc ggcctccgag gggccgaagt cgaaggcgat ccgcaccgtc tcggcgcccc
      961 gcgtgatctc gacgaagtgg gcgaggccgt gctcggcccg cagatccacc atctggcgcg
     1021 ccacgaaggc gctgtgccgg tgggcgacct tctcgtcctg ccggagggcg tcgatgtagt
     1081 tgtccaccac cgtggtgacg cgaacccggt cgaccggggt gagcggggcc atcagtgcac
     1141 ccggctccgg acaacgtcca tcagggactc gtagggagac atcattcttt ctcctagtat
     1201 tcgctcggct cggcctcggc cgcgcctcgc acctgttcat gccagagccc cagcgcgcgg
     1261 agcgcgggct cgtcgttgag gtggaagagg cacgcggggg cctggcccga ccggttgcga
     1321 tgggcggtcc agtgccacgg cggcaccacg aagacgtcgc cggcctgcca gtccagcgtt
     1381 cgatcgccga cgcggctctc gccctgcccc tcgatgacgt ggaccacctc gctcgcggtg
     1441 cggcgctccg cgcgcgtctc ctcgccgggc cgcagccagc gcgcctcgga gcccatcgtc
     1501 cggagcgggt actcgccggt tcgcgggttc acgtggcgca gcaccaccgg cgcgcgccgg
     1561 ggtgcggtgg cggccatggc cgcgagcgcg gcgcgtgcgg tgcgccacgg ccagcgcacc
     1621 tgtgggtaac cggtctcctc gtaccgcgat cggcgcggca cgaggcccgc cgcggtgaat
     1681 tcgtcctggg aggagtcggt ctcggtggcc ggggtggacg cgggcctcat ggccgaggcc
     1741 cagttcgcct cgacgttgcg caccagcggg atgtcgaggc cgtccagcca gatcaccggc
     1801 tcgcggccct cgtggccgtg gtcgtgccag cgcatcggcg gggtgatgat gaaatcgccc
     1861 ggctccatcg cgcacttcac gccgtccacc gtggtgtagg cgcccgcccc ctcgacgatc
     1921 aggcgcagcg ccgccgcggt gtgatggtga ctgggtgccg cctcgccggg caggatgatc
     1981 tgcaggcccg cgtagagcgt ggcggtcgcc gagaagttgc ccggcaggcc gggattgcag
     2041 agcaccagca cccgccgctc cgcctgctcg atcggcacca ggcgcgccgc ctcgagcagg
     2101 ggcgcgcgca gctcgctcca ccgccagcgg tgcggcacca cccgcgtctg ccgctcgggc
     2161 ggcaggagcg tgtggagcgc ggtccacagc ggcgccagcg agagggcctc gagccggtcg
     2221 gagtacgcgg cgggcagctg ggcgtcgcgc gtcgagagcg tcatgaccgc ggggctccgc
     2281 ggctcaacaa tgcttgcatg gggggaactc tcgcgaatag accggctgct cgaggaactt
     2341 ctgcggatcg taggtccaga actggctcac cgccggatag gtgtggatca cggtgttctg
     2401 cagcaccccg ccgaccttct cgaccttgcg gacgtagatg ttctgggtcg ggttgccgta
     2461 ggcgtcgatc ttgaccggtc cgcgcggggc gtcggtgatc tccacccgac gcagcgcggc
     2521 catcaacgcg gcccggtcct ccacgcgccc gttcaccgcc ctcacggcct cctcgatcac
     2581 gcgggcgccc gtgtagcagt tctccgagta gaatgacggc gagcgcgagt acgccttctc
     2641 gaacgcggcg cggaacttcc ggttggccgg cgtgtcgagc gcggccgagt agtggtgcgc
     2701 gctgatcacc ccgattgccg catcgccgag ctgggagagc acgtgctcgt ccgtatgggt
     2761 cccgcccgcg atcaacggga cccgcccctt gaggttgctg tccgcgtact gcttggagaa
     2821 cagcacgctc cagcgtccga gggcgagcac gaagaccgcg tccgcgtccc gcttcacctg
     2881 ggccaggtac ggcgcgtagt cctggatatt gagcggcacc cagatcttct ggatgatgtg
     2941 gccaccgttg tcctcgaagg ccttgtggaa cccgccgacg gtctcccacc cgaaggcgta
     3001 gtcgaggctg atcgtgacca ctttccggta cttcagcacc ttcgccgcgt actcaccgaa
     3061 cgggtgcatg ttgccgctgg ccgagaagcc ggtgcgcacg agccacgtgg gccgcttgcg
     3121 ctgggtcagg tcgtcggccg agttgatcgg atagatggtc gggatgcccg cggcctcgat
     3181 cggcggcacc agggcgtagg ccacgttcga gaggatgacc ccggcgagca gattgacccg
     3241 gtcgctgtcg atcagcttcc gcagcttggt caccgcggtg gcgggctggc cctcggtgtc
     3301 ctccaggatg acctcgagct tccgaccggc gatctgcatc ccgcgctcct gccagtacag
     3361 ctcgcagccg ctgtacatgt ccttcccggc ctgggcgatg gcgcccgaga tcggcgagag
     3421 gaagccgatc ttgatcgggc cctgggccac cgccggcccg gggcccaccg ccacgaccag
     3481 cccgaccacc gcgaccagca tccggatcgt gcgcattcgt cggctcctcc gttggagtac
     3541 gggtccgagt ccgggacagt ctaccgcttc ggcaccgggt ggggtatatg agccagatgc
     3601 gcgtggagtt cgtgctgacc gcggtggtgg gcctgggcgc cctgtccatc gacatgttcc
     3661 tgccgtcgct gcccaccatc gcggccgtgt tcggcgccga ggccgcggtc gcgcagctca
     3721 cggtgaccct gttcctgctg gccttcgcgg cctcccagct cgtctacggg ccgctgtccg
     3781 accgcttcgg ccgccgctgg gtgctgatcg gtggcctcgc cctctactcg gtggccggcc
     3841 tcgcctgcgc gctcgcgccc acgatcggcg tgctgatcgg cgcccgcgtg ctccaggccc
     3901 tgggcggcgg caccggcccg gtggtggcgc gcgcggtgat ccgcgacctc tacgaccgcg
     3961 agcgtgccgc gcgcgtcttc tcctacatgg gcatgttcca gtccctcaat ccgatgctgg
     4021 cgccgatcgt gggcggctac gtgcacgagg ccttcggctg gcgcgcgatc ttcttcgtgc
     4081 tggcgggcgc gggcgcgctc ttcgtggcgc tgatgagcgt gggggtgccc gagaccaatg
     4141 tccgccgcga tcccaccgcg ctgcggccgg ggcagatggg ccgcaacgtg gcggagctgc
     4201 tcaccgaccg gagctacgtg gcctacgtgc tggtgaacac gctgatgttc ggcggccagt
     4261 tcgccttcat ctccggctcc tccttcgtgc tgatcgacgt gctcggcgtc tccccgagcg
     4321 ggttcggact ctgcttcggc tcggtcgcgg tcggcatcat gaccggcacc ttcctctccg
     4381 gccggttcgg cgcgcggatc cagctcgatc gcacgatcct ctccggcacc gcgctcggcg
     4441 ccgcggccgg attgatcctc gccgcgctgg cctggagcgg gatcctgacg gtggcggcgg
     4501 tgatcgtccc gatgttcgtg ttcgcggtcg ggctcgggct caccctgccc aacggcatgg
     4561 ccggcgcggt cggcccgttc ccgcgcatgg ccggcctggc cgccgcggtg gccggcttcc
     4621 tccagatgac gggctcggcg ctctacagcg tggcggtggg gcacttctac gacggcaccg
     4681 cccgcccgat gaccgccgcg atcgcgctgg ccggcgtcgc cgcgctcgtc tgcttctggc
     4741 gcctccggtg gaggcgggcg cacggcgtgc tagcatgagc accatggccg cgcgccgcgc
     4801 gaagatcatc cgcttcccga cacccccacc gaaggcgccc gcgcgcgccg cgacggagct
     4861 ggtggaggcg cggcgctgcc gggatcagac ggaggcgctg gtggtgcgcg ggctgctcga
     4921 gagcgagggc atcgcggtcg tcccgcggag ccgcctcgcg ccctcggtct acccgttcag
     4981 cgtgggccag caggccgagg tcgtgctgct ggtcgccgcc gcggatctgc cgcgggcgct
     5041 cgccgtgctc gcgcgccagg cgtagagccg ctcgggggga ccacccctca ccctgccctc
     5101 tcccctgagg ggagaggggc cataggatca actttacttg tagaacttgt ccgggtcggg
     5161 gtcctgcttg ctgttgaagg cccggcccat ctccttggcc tcctcggtgt ggtagtagtt
     5221 cgccaccagg tggtcgatgg tcacgcggga gagccccgtg acgccgttgt gccgcgcgtg
     5281 gaaggcggcc ttcagcgcgg ccagggcctg cggcccgcgc ttcttcagct cctcgcacat
     5341 ggcctcggtg cgcgcgcgca actcggcgtg gggcgccacc tcgttgacca gtcccatggc
     5401 ctgcgcttcc ttcgcggtgt acttgcgatt gagataccag atctccttcg cgcgcttgcg
     5461 gccgaccgag tcctcgaggt accaggtgcc gaagcccgcg tcgtaggagc ccatggccgg
     5521 acccacctgg cggaacgtgg cctgctcgct cgccaccgtg agatcgcaca tgttggcgag
     5581 cacgttgccg ccgcccaccg agaagccgtt gaccatggcg atgatcggca ccggcatcat
     5641 gtcgatcagg gtgtagagat ccaccaccgg cggcacgttg tggtagtgca gcaggccatc
     5701 gttgggctcc ttctcgccgc cgatgcagaa gaagcggtcg cccgcgccgg tgatcaccac
     5761 cacccgcacc cccgcgtcgt tgcgcgcccc gttgagcgcc tggatcatct cgagcacggt
     5821 ctggcgccgg aaggtgttgc ccttgtcggg ccggttgatc gtgagccacg cgatctgatt
     5881 cttgacctcg aagaggatgt ccgagtaggc catgaggcga ctcctttgtc gacagggtta
     5941 gcgggtcggg atccggaaca gccggtgcgc gttctcggtg acggcctcgc cgagcgcctc
     6001 gggcgagacg ccccggagcc cggccacgtg agcggcggtg atggccaccc acgcgggctc
     6061 gttgcgctgc ccgcgatggg gatgcggcgg gaggaagggg caatcggtct ccaccaccag
     6121 acggtcggcg ggcacgaagc gggcgacgtc ggggagcgcc cgcgcgttct tgtaggtcac
     6181 cggcccggcg agcgagatca tgagccccag atcgaggcag cgcttggcca cctccacgtc
     6241 ggcggagaag cagtgcatca cgccgcccac ctcgcccaca tgctcgtccg aaagaatcgc
     6301 gagggcttcc cggtgcgcgt cgcggcaatg gatgacgacc ggcttgccga gccgccgcgc
     6361 catgtcgagc tggcggcgca gcgccgtctc ctgcgcgtca cggggcgaga ggttgcggaa
     6421 gaagtcgagc cccatctcgc ccagcgccac caccttggcc gacccgcggg ccaggcgctc
     6481 catcgcctcg aagtccggct ccgtggcctc cccggcgtcg tggggatgga tgcccacggt
     6541 ggcgtgcacg tcatgcagtc gctcggccag cgccaccgcg gcgggattgg tctcgcgatc
     6601 ggtgccgatg gtcaccatcc cggtcacccc ggcggcccgc gcgcgctcca gcaccgcgtc
     6661 gaggtccgcc agcagctcgg ggaagtgcag atgcgcgtgc gtgtcgaaga gcggcatcgg
     6721 tcggtccgcc gcaatgatat cagggggcga cggtgaagcg caggcggc
//
LOCUS       DASUFA010000178         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_614156, whole genome shotgun sequence.
ACCESSION   DASUFA010000178 DASUFA010000000
VERSION     DASUFA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614156"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..930)
                     /locus_tag="VFX87_06365"
     CDS             complement(97..930)
                     /locus_tag="VFX87_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4994572.1"
                     /translation="MAGGAGLIRRLALSAAGLLLAAVLLAPYGVMLLTALKPEAELRV
                     SPPRLLPLEWRPQNFWTVVRDPEFRSWLRVSLVVALASTALVLGAAVPAAYHTARHRF
                     PGRGAFLFLVLVTQMFAPTALVVGLYREFFELGLISTYTALILVNAAFNLAFAIWILR
                     AFFASIPREIEEAAAVDGCGSAGVLFRIILPVALPGIVTAAIFAFIAAWNEYIVALTL
                     MTDASMRPLTVGITSYVTAYVQHWDLLFAASVIAIVPVVVLFALIEPYLVGGLTAGSV
                     K"
     gene            complement(909..1718)
                     /locus_tag="VFX87_06370"
     CDS             complement(909..1718)
                     /locus_tag="VFX87_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX4994573.1"
                     /translation="MIYPAVELVRASLGRYSITGLYQGAVGLRNYARLLEQEALPAVA
                     GNTAVWVAVVVALTIALSLGLAQLLNESFPGRRLVRWALIVPWAASLIMTSKLFVWLY
                     DYYFGLLNRVMTVAGLAPLDWLGDDRTVMGAMIAVGVFVSLPFTTYVLLAGLQTIPGE
                     VYEAARVDGAAGWRSYRMITLPLLRPALLVAAVLNLIYVFNSFPIVWTLNDRNPGFAH
                     DTTITFMYKIAFKSALRDVGLAAALGVVNVLLILVAVVAYLRVARWREEPA"
     gene            complement(1799..2125)
                     /locus_tag="VFX87_06375"
     CDS             complement(1799..2125)
                     /locus_tag="VFX87_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994574.1"
                     /translation="MAFKKKGNQEAVRRFLDLYYQPEQITRWITAEGFLPVTKSGRER
                     MSGNEKLKPYLDALPSARLAPTTDPTWDKVKLDVQQSIGAAVQPGGDPKQVLDRLQKN
                     AAESAR"
BASE COUNT          404 a          770 c          804 g          280 t
ORIGIN      
        1 cgcagggact tcgaaaccgc cgatcagcga gtccgggaag gccccccgcg gccggcccac
       61 ctccgccctg cgtgccgcgc gcctaacgga gacgagctac ttgacggacc ccgcggtgag
      121 cccgccgacg aggtagggct cgatgagcgc gaagaggacg accaccggga cgatcgcgat
      181 gaccgaggcg gcgaagagca ggtcccagtg ctggacgtag gcggtgacgt aggaggtgat
      241 gccaaccgtg agggggcgca tcgaggcgtc ggtcatcagg gtgagagcga cgatgtactc
      301 gttccaggcc gcgatgaagg cgaagatggc ggcggtcacg atgccgggga gcgccaccgg
      361 caggatgatg cggaagagga cgccggccga tccgcagccg tcgaccgcgg cggcctcctc
      421 gatctcgcgc gggatcgagg cgaagaaggc gcgcaggatc cagatcgcga aggcgaggtt
      481 gaaggccgcg ttgaccagga tcagcgcggt gtaggtgctg atcaggccca gctcgaagaa
      541 ctcgcggtag agccccacca ccagcgcggt cggcgcgaac atctgggtca ccaggaccag
      601 gaacaggaag gccccgcgcc ccgggaagcg gtgccgggcg gtgtggtagg ccgcgggcac
      661 cgcggcgccc agcaccagcg cggtggacgc gagcgccacc accagcgaga cccggagcca
      721 ggaccggaac tccggatcgc gcaccaccgt ccagaagttc tgcgggcgcc actcgagcgg
      781 cagcaggcgc ggcgggctga cgcgcagctc ggcctccggc ttcagcgcgg tgaggagcat
      841 caccccgtag ggcgcgagca gcaccgccgc cagcagcagc cccgccgcgc tcagcgcgag
      901 cctgcggatc aggccggctc ctcccgccac cgcgccaccc gcaaataggc gaccaccgcg
      961 accaggatca gcagcacgtt gaccacgccc agcgcggcgg ccaggcccac gtcccgcagc
     1021 gcgctcttga acgcgatctt gtacatgaac gtgatcgtgg tgtcgtgggc gaagccgggg
     1081 ttccggtcgt tgagcgtcca cacgatcggg aaggaattga acacgtagat caggttcagc
     1141 accgcggcga ccagcagcgc gggccggagc agcggcagcg tgatcatccg gtagctgcgc
     1201 cagcccgccg ccccgtccac ccgggccgcc tcgtagacct cgccgggaat ggtctggagc
     1261 cccgcgagca ggacgtaggt cgtgaacggc agcgacacga acacgcccac cgcgatcatc
     1321 gcgcccatca cggtgcggtc gtcgcctagc cagtcgagcg gcgccaggcc cgccacggtc
     1381 atcacgcggt tgagcaggcc gaagtagtag tcgtagagcc agacgaagag cttcgaggtc
     1441 atgatgagcg aggcggccca cggcacgatg agcgcccacc ggaccagccg gcggccgggg
     1501 aacgattcgt tcaggagctg ggccagcccg agcgagagcg cgatggtgag cgccaccacc
     1561 accgcgaccc agaccgcggt gttcccggcc accgccggca gggcttcctg ctcgagcagg
     1621 cgcgcgtagt tgcggaggcc caccgcgccc tggtagagac cggtgatcga atagcggccg
     1681 agggaggcgc gcaccagctc gacggcgggg tagatcacca ccgccgcgat caggacgagg
     1741 ctcgggccga gccagaggag cgcccggccc gcgcccgggc cccgcgggcg cgggcccgtc
     1801 agcgagcgct ctccgccgcg ttcttctgga ggcggtccag cacctgcttc gggtcgccgc
     1861 ccggctggac ggccgcgccg atgctctgct ggacgtcgag cttcaccttg tcccaggtgg
     1921 gatcggtggt gggcgccagt cgcgcgctcg gcagcgcgtc gaggtacggc ttgagcttct
     1981 cgttgccgct catgcgctcg cggcccgact tggtcacggg caggaagccc tcggcggtga
     2041 tccagcgggt gatctgctcg ggctggtagt acagatcgag gaaccgacgc accgcttcct
     2101 ggttgccctt cttcttgaag gccaagcaag agcgacggcc gatacgcgag tcgacagact
     2161 caggatagac atgcaaccct ccatcgttca gtcgtcagcg acgagacgaa cgcatcttta
     2221 tacacccatt gccgatgtac ttcccggcct tccacgga
//
LOCUS       DASUFA010000179         5500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_371793, whole genome shotgun sequence.
ACCESSION   DASUFA010000179 DASUFA010000000
VERSION     DASUFA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371793"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VFX87_06380"
     CDS             <1..1114
                     /locus_tag="VFX87_06380"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4994575.1"
                     /translation="ATAALNPSDIATPLRSILRPATNITVLLAEVRSVDLAGRRVILD
                     EGEVTYDALVLAAGAGNSYFGHDDWEELAPSLKSLEDALEIRRRVLLAYEDAERERDG
                     AEQRALLTSVVIGGGPTGVELAGALAEISRETIARDFRLIDPTKARIVLVEAGPRLLP
                     SFPAPLSAHAGAALARLGVEVRTGAPVTQVTPDAVWLGGEQIRARTVLWAAGVAASPL
                     ARSLGVPLDRAGRVLVEPDLSVPGHAMVFVIGDMSSLRGPDGHPLPGLAPVAIQQGQA
                     VAANVRRRLAGLPTRPFRYRDKGSMATIGRAAAVAVVGRVQLHGLPAWLAWLFVHIMF
                     LIGFRNRFLVLFEWAWAYLSFQRGARLITGHWKPRR"
     gene            1196..2809
                     /gene="glgP"
                     /locus_tag="VFX87_06385"
     CDS             1196..2809
                     /gene="glgP"
                     /locus_tag="VFX87_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX4994576.1"
                     /translation="MSAPARPPLVAYFSMEYGLHEEFHSYAGGLGVLAGDFMKSAGDL
                     GLPVVGIGLRWGQGYTSQHIGADGYPHDEWTDQKPDVLTDTGARVRVRVAAREVECRV
                     WRVGRYAIAPLYLLEPTDPRDLWITRRLYDPRPDCRVAQEMLLGIGGVRALRALHLPV
                     ERYHFNEGHAVFAGIEMIADRMEAGQTFRDAWLVARERIVFTTHTPVPAGNEVHPLAD
                     LRRLGAGCELVPSELAEIGGDPFNMTVAGLRLARLANAVAQLHGETARAMWAHVKDAA
                     PIIAITNGVHAGTWQDARIPAAQASDDRLRAAKRALKTELLAEVLTRTGVRLDPAAPT
                     IGFARRAATYKRPDLLLRDPERFAALVKRHRIQVLFAGKAHPADHEGKAVIARLVAAL
                     RQWPESVAFLEDYDMALGRLLTRGADVWLNTPRRPMEASGTSGMKAAMNGTLNLSVLD
                     GWWPEGCEHGVTGWAIGDETTPADPDARDLRALYDTLEGLVLPAFADPARWTTMMRAS
                     IRMAVERFSSDRMVQEYFTRLYAPTSLAR"
     gene            complement(2790..3242)
                     /locus_tag="VFX87_06390"
     CDS             complement(2790..3242)
                     /locus_tag="VFX87_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994577.1"
                     /translation="MARAALLLLSLLAVAAPVAADMTPAPPRNRMPPAPSAEQEYARG
                     LAATRAREWSVAVAAFNRAVEMKPAYPEAWNGLGHALRQQGRYPESLKAYDEALRLRP
                     DYPEALEYLGEAYVKLGRLDDARRVLERLRPLDASRARELAEAIAQAK"
     gene            complement(3245..4342)
                     /locus_tag="VFX87_06395"
     CDS             complement(3245..4342)
                     /locus_tag="VFX87_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994578.1"
                     /translation="MLAALTRGAAAGAGAAAAEGGAARHQVPDDLLAFQGFMMERGWG
                     DGLPMIPATPERVAAMLAATGRPAGDLVAILPPRLGRATVERVAVNAVLAGALPEHFP
                     VILAAVEAVAQPAFNLPAIQTTTHPCSPLIIVNGPIAARLGINGAGNALGQGHRANAV
                     IGRALRLVLQNTGGARPGSEDRATQGHPGKYTYCVAENEAASPWEPLSVERGFPADVS
                     TVTVCASESPHNINDHGSSTPEGILTTVAGTAATVGSNNIYLGGEPLVVLGPEHAATV
                     AGGGWSKADFKRALWERAQVPLSRFDPSNLARFAVIHPAGFKDRPPETLAPLARAWTD
                     IMIVVAGGAGKHSVLIPTFGATRSVTLRIEA"
     gene            complement(4559..4837)
                     /locus_tag="VFX87_06400"
     CDS             complement(4559..4837)
                     /locus_tag="VFX87_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994579.1"
                     /translation="MEILDPVGSTSAASRTLAPRPASLAGAVIGVLDNSKPNARVLLE
                     GVARALAQKVGARDVAVWGKPGASIAATPAVLDEIAAQCGAVLTASAD"
     gene            complement(4846..>5500)
                     /locus_tag="VFX87_06405"
     CDS             complement(4846..>5500)
                     /locus_tag="VFX87_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994580.1"
                     /translation="VRAEPREHDARTSVPIQFLAGMREVLAMPALWPFNLYVLFSYGG
                     YFALLTWLPTFLVKSEGLRQAGAGFVTSLITGGTIVSWPLAGFLSDRFGRRRPIFLVS
                     QAMSVLVCLAFAYLVPGTGLAGAAAVAALTGLLLGGLITPFVMVTELVPPRLMGTASG
                     VVNAFCFVGGLLVPVALGYALDLTGSFPAAFTACAVFEVVALAIAAFARETGRRAAV"
BASE COUNT          740 a         2044 c         2002 g          714 t
ORIGIN      
        1 cgccaccgcc gcgctcaatc cgtccgatat cgcgacgccg ctgcgctcca tcctgagacc
       61 cgcgaccaac atcaccgtgt tgcttgccga ggtccggtcg gtggacctgg cgggccggcg
      121 cgtgatcctg gacgaagggg aggtgaccta cgacgcgctc gtgctggccg cgggggcggg
      181 caactcctac ttcggccacg acgattggga ggagctcgcg ccgagcctca agagtctgga
      241 ggacgcgctc gagatccggc gccgcgtgct gctcgcctac gaggacgcgg agcgcgagcg
      301 cgacggggcc gagcagcggg cgctcctgac ctccgtcgtc atcgggggcg ggccgaccgg
      361 ggtcgagctc gcgggcgcgc tcgcggagat ctcgcgcgag accatcgcgc gggacttccg
      421 gctcatcgat ccgacgaagg cccggatcgt cctcgtcgag gccgggccgc ggctgctccc
      481 cagctttccc gcgccgctat ccgcccacgc gggcgccgct ctcgcgcgcc tcggggtgga
      541 ggtccgcacc ggcgcgccgg tcacgcaggt gacgcccgat gcggtctggc tcgggggcga
      601 gcagatccgt gcgcgcaccg tgctgtgggc ggcgggggtg gccgcctcgc ccctcgcgcg
      661 ctcgctcgga gtgccgctcg atcgcgccgg ccgggtgctc gtggagccgg atctctcggt
      721 gcccggtcac gccatggtgt tcgtcatcgg cgacatgtcc agcctccgcg ggcccgacgg
      781 gcacccgctc ccgggcctgg ccccggtggc catccagcag gggcaggccg tcgccgcgaa
      841 cgtgcgacgg cgcctcgccg gcctcccgac ccggcccttc cgctatcgcg acaagggcag
      901 catggccacc atcggccgcg ccgccgcggt cgcggtggtc ggccgcgtcc agctgcacgg
      961 cctgcctgcg tggctcgcct ggctcttcgt ccacatcatg ttcctgatcg gcttccgcaa
     1021 tcgtttcctc gtcctcttcg aatgggcctg ggcctatctc agcttccagc gcggcgcgag
     1081 gctgatcacc gggcactgga agccgcggcg ctgagccgac cacacgcgcg gggcgggttt
     1141 cgcggaggcc ccccgcccca ccccgttgta tgctcgaaga cgtgagatcg ctccgttgag
     1201 cgccccggcc cgtcctccgc tggtcgccta cttctcgatg gagtacggcc tgcacgagga
     1261 gttccactcc tacgcgggcg gcctcggcgt gctcgcgggc gacttcatga agtcggcggg
     1321 cgatctcggc cttcccgtgg tcggcatcgg gctccgctgg ggccagggct acacctccca
     1381 gcacatcggc gccgacggct atccccacga cgagtggacc gaccagaagc ccgacgtgct
     1441 caccgacacc ggcgcgcgcg tgcgcgtgcg ggtcgcggcc cgcgaggtcg agtgccgcgt
     1501 ctggcgcgtt ggccgctacg ccatcgcccc gctctacctg ctcgagccca ccgatccgcg
     1561 cgacctgtgg atcacccggc gcctctacga cccccgcccg gattgccgcg tggcccagga
     1621 gatgctgctc ggcatcggcg gggtgcgggc gctgcgggcg ctccacctgc cggtcgagcg
     1681 ctaccacttc aacgagggcc acgccgtctt cgcgggcatc gagatgatcg cggaccggat
     1741 ggaggcgggg cagaccttcc gcgacgcgtg gctggtcgcg cgcgagcgca tcgtcttcac
     1801 cacccacacg ccggtccccg cgggcaacga ggtgcacccg ctcgcggacc tgcgccgact
     1861 cggcgccggc tgcgagctgg tgccgagcga gctggccgag atcgggggcg atccgttcaa
     1921 catgacggtg gccggcctgc ggctggcccg cctggccaac gcggtggcgc aactgcacgg
     1981 agagaccgcg cgcgccatgt gggcgcacgt gaaggacgcg gcgcccatca tcgcgatcac
     2041 caacggggtc cacgccggga cctggcagga cgcgcggatc cccgccgcgc aggcgagcga
     2101 cgaccggctg cgggccgcca agcgcgcgct caagaccgag ctgctcgcgg aggtgctgac
     2161 gcgcaccggg gtgcggctcg atccggccgc gccgaccatc ggcttcgcgc ggcgcgccgc
     2221 cacctacaag cggcccgacc tgctgctgcg cgatccggag cgcttcgcgg ccctggtcaa
     2281 gcgccaccgg atccaggtcc tcttcgcggg caaggcgcac cctgcggatc acgagggcaa
     2341 ggcggtgatc gcccggctcg tggcggcact gcggcagtgg cccgagagcg tggcgttcct
     2401 cgaggactac gacatggccc tgggccgcct gctcacccgc ggcgccgacg tctggctgaa
     2461 cacgccgcgc cgcccgatgg aggcgagcgg cacctccggg atgaaggcgg cgatgaacgg
     2521 cacgctgaac ctctcggtgc tcgacggctg gtggcccgag ggctgcgagc acggcgtcac
     2581 cggctgggcg atcggcgacg agacgacccc ggccgatccg gacgcgcgcg acctgcgcgc
     2641 cctctacgac acgctcgagg gcctagtcct gcccgcgttc gcggatcccg cgcgctggac
     2701 caccatgatg cgcgcctcga tccggatggc ggtggagcgc ttcagctcgg accgcatggt
     2761 gcaggagtac ttcacccgcc tctacgcgcc tacttcgctt gcgcgatagc ctcggcgagc
     2821 tcacgcgccc ggctcgcgtc cagcggccgc aggcgctcca gcacgcggcg agcgtcgtcg
     2881 aggcggccca gcttcacgta ggcctcgccc aggtactcga gcgcctcggg atagtcgggc
     2941 cgcagccgca gcgcctcgtc gtacgccttg agcgactccg ggtagcggcc ctgctggcgc
     3001 agggcatgac cgagcccgtt ccaggcctcc gggtaagccg gcttcatctc gacggctcgg
     3061 ttgaaggcgg cgacggccac gctccattcc cgggcgcgcg tggccgccag cccgcgggcg
     3121 tactcctgct ccgccgacgg cgcgggcggc atccggttcc gtggcggggc gggcgtcatg
     3181 tcggccgcga ccggcgcggc caccgccagg agcgacagca gaagcagggc cgcgcgtgcc
     3241 acgctcaggc ctcgatccgg agggtgacgg agcgcgtagc gccgaaggtc gggatcagca
     3301 cggagtgctt gcccgccccg cccgccacca cgatcatgat gtcggtccac gcccgcgcga
     3361 gcggggccag cgtctcgggc ggccggtcct tgaacccggc cggatggatc accgcgaagc
     3421 gggccaggtt ggagggatcg aagcgcgaga gcgggacctg cgcccgctcc cagagggcgc
     3481 gcttgaagtc cgccttgctc cagcctccgc ccgccacggt ggccgcgtgc tccggcccca
     3541 gcacgaccag cggctccccg cccaggtaga tgttgttcga gccgacggtc gcggcggtgc
     3601 ccgccaccgt ggtgagaatg ccctcggggg tggacgagcc gtggtcgttg atattgtgcg
     3661 ggctctcgga ggcgcacacc gtgacggtgc tcacgtccgc cggaaacccg cgctccaccg
     3721 acagcggctc ccacggcgag gccgcctcgt tctcggcgac gcagtaggtg tacttgccgg
     3781 gatgcccctg ggtcgcgcgg tcctcgctcc ccggccgcgc cccgcccgtg ttctgcagca
     3841 ccagccggag cgcgcgcccg atcaccgcgt tggcgcggtg gccctgcccg agcgcattgc
     3901 cggcgccgtt gattcctagc cgcgccgcga tcgggccgtt gacgatgatc agcggcgagc
     3961 agggatgcgt ggtggtctgg atcgccggca ggttgaacgc gggctgggcc accgcctcca
     4021 ccgccgccag gatcaccggg aaatgctcgg ggagcgcgcc ggccagcacc gcgttgaccg
     4081 cgacacgctc caccgttgcg cggccgagcc gcgggggcag gatcgccacc agatcgcccg
     4141 cgggccggcc ggtggccgcg agcatggccg cgacgcgctc gggggtggcg gggatcatgg
     4201 gcagcccgtc gccccagccg cgctccatca tgaagccctg gaaggcgagc aggtcgtcgg
     4261 ggacctggtg ccgggccgcc ccgccctcgg cggccgccgc acccgcgccg gccgccgcgc
     4321 cgcgggtgag ggcggccagc accgcatcga ccgcgttcag ggccttgccc gcgacgatcg
     4381 cctcggcgat gccgccgatg ggatgcggga cggtgacgat gccgagatcg ggaagcccct
     4441 tggccttcgc ctcggcgagc gcgagcggcc ggaactcatg cgtgcccagg acgacggtgg
     4501 cgatcccccg cccttcgagc tgcgtggcgt cgtggatact ccacgacgtg cacgaccctc
     4561 agtcggcgga ggcggtgagg acggcgccgc actgcgcggc gatctcgtcc aggacggcgg
     4621 gggtggcggc gatcgaggcg ccgggcttgc cccacaccgc cacgtcgcgg gctccgacct
     4681 tctgcgcgag cgcgcgggcc acgccttcca gcaggacgcg ggcattgggc ttcgagttgt
     4741 cgagcacgcc gatgaccgcg cccgccagcg aggcaggccg tggggcgagc gtccgggacg
     4801 ccgcggaagt cgagccgacc gggtcgagga tctccatggc cgacatcata ccgcggcccg
     4861 gcggccggtt tcccgcgcaa acgccgcgat ggcgagggcc accacctcga agaccgcgca
     4921 ggcggtgaag gcggcgggga agctcccggt caggtcgagc gcgtagccca gcgccaccgg
     4981 gacgagcagc ccgccgacga agcagaaggc gttgaccacc ccggacgcgg tccccatcag
     5041 ccgcggcggc accagctcgg tcaccatcac gaacggggtg atcaggcccc cgaggaggag
     5101 cccggtcaac gcggcgaccg ccgccgcgcc cgcgaggccg gtgcccggca ccaggtaggc
     5161 gaaggccagg cacaccagca cgctcatcgc ctggctcacc aggaagatcg gcctgcggcg
     5221 cccgaagcga tccgaaagga acccggcgag cggccacgag acgatcgtgc cgccggtgat
     5281 gagcgaggtc acgaagcccg cgcccgcctg gcgcagcccc tccgacttca ccaggaaggt
     5341 gggcagccag gtgagcagcg cgaagtagcc gccgtaggag aagagcacgt agaggttgaa
     5401 gggccagagg gcgggcatcg cgagcacttc gcgcatcccg gccaggaact ggatgggcac
     5461 gctcgtcctc gcgtcgtgct cacgcggctc ggcccgcacc
//
LOCUS       DASUFA010000180         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_42868, whole genome shotgun sequence.
ACCESSION   DASUFA010000180 DASUFA010000000
VERSION     DASUFA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42868"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /gene="hisH"
                     /locus_tag="VFX87_06410"
     CDS             <1..583
                     /gene="hisH"
                     /locus_tag="VFX87_06410"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX4994581.1"
                     /translation="LGSVEKAFARIGMPAVVTQDPKVVDDAEAVVLPGDGAFHDAMGS
                     LQALGLIAPLRACFESERPFFGICLGYQLLFSESEEFGQGKGLDVIAGAVRRFPKGLK
                     VPHMGWNQVEHRGDLRLFDGIPSGAHFYFVHSYYPETADTSLRVATCTYGLTFPAAVS
                     RGTLYATQFHPEKSQRWGLRMLENFAAIVRESR"
     gene            731..1450
                     /gene="hisA"
                     /locus_tag="VFX87_06415"
     CDS             731..1450
                     /gene="hisA"
                     /locus_tag="VFX87_06415"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX4994582.1"
                     /translation="MFEVVPAVDLKAGRCVRLLQGRADAETVFSDDPVAMARRWQDSR
                     APRLHVVDLDGAFAGRPSQTDLVRAMIAAVRIPVQVGGGLREIAHVEAVLEAGARWAI
                     VGTRAALDPAFLGQVCERFGEQVIVGIDASDGRVAVDGWTRVLDLDAIALARDAAAAG
                     AAGIIYTDIARDGTQNGPNLWSTEAVAKAAGIDVFASGGVGSLDDIRQLATVPELAGV
                     IVGRALYSGAVDLGAALAAVR"
     gene            1457..2218
                     /gene="hisF"
                     /locus_tag="VFX87_06420"
     CDS             1457..2218
                     /gene="hisF"
                     /locus_tag="VFX87_06420"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX4994583.1"
                     /translation="MLCKRLIPCLDVKDGRVVKGVRFVELRDAGDPVAAALAYDAQGA
                     DELVFLDITASHEGRATMLDVVRRTAEGIYMPLTVGGGVRSLEDIRTLLRAGADKVSL
                     NTAALERPGLVSEAAHAFGSQCIVVAIDAKSSNRHWEVFTHGGRRPAGRDAVEWAREV
                     EKLGAGEILLTSMDRDGTGDGYDIALTRAVAEAVSVPVIASGGVGRLEHLREGLVEGR
                     ADAALVATLFHFGQHTVPEAKRYLHARGVPVRLEP"
     gene            2215..2868
                     /gene="hisIE"
                     /locus_tag="VFX87_06425"
     CDS             2215..2868
                     /gene="hisIE"
                     /locus_tag="VFX87_06425"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEX4994584.1"
                     /translation="MSPTDDLKWDAQGLIPAVVQDTETGELLMVAWMSAESLAETRRT
                     GLTHFWSRSRNALWRKGETSGHVQHVDGLYADCDRDTLLVQVHQEGVACHTGARSCFF
                     TRLDGGEAAPASGAAGPAMLEVLERVLESRKVAPPPGSYVAGLFEKGQPQICRKIGEE
                     AAEVMTAALGGEGDERVVSEVADLWFHTMVLLSARGIPLRRVFQELTRRHKQDKKDT"
     gene            2865..>3176
                     /locus_tag="VFX87_06430"
     CDS             2865..>3176
                     /locus_tag="VFX87_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994585.1"
                     /translation="MTGHPLARALLALILLAGCATATGRIENGMFYSAKGYRVTLPPA
                     GWRVDAGQRADLALRGEARAGGMVVDATCSGRESLRPLDVLARHLTFGLTRRDVLENG
                     TS"
BASE COUNT          437 a         1080 c         1205 g          454 t
ORIGIN      
        1 tctcggcagc gtggagaagg ccttcgcgcg gatcgggatg cccgccgtcg tcacccagga
       61 cccgaaggtg gtggacgacg cggaggcggt ggtgctgccc ggcgacgggg cctttcacga
      121 cgcgatgggc agcctccagg cactggggct gatcgcgccg ctccgggcct gtttcgagag
      181 cgagcggccg ttcttcggga tctgcctggg ctaccagctt ctcttctcgg agagcgagga
      241 atttggccag ggcaagggcc tcgacgtcat cgcgggcgcg gtgcgacgct tccccaaagg
      301 cctgaaggtg ccgcacatgg ggtggaacca ggtcgagcac cggggggacc tgcggctctt
      361 cgacggcatc ccgagcgggg cgcacttcta cttcgtgcac tcctactatc cggagaccgc
      421 cgacacctcc ctccgggtgg cgacctgcac ctacggcctc acgtttccgg cggccgtctc
      481 gcgtggaacc ctctacgcga cccagttcca ccccgagaag agtcaacgct ggggcctccg
      541 catgctcgag aacttcgcgg ccatcgtccg cgaatcgcgc tgaccgggat cgagatggcc
      601 gaggtgcagc cgaaggcgag ccgaggagac catgattccg aacgggaatg gatggtcgtc
      661 gaatggccga ggtgcagccg aaggcgagcc gaggagacca tgagccgtcc agggagtggt
      721 aggtagtcca atgttcgagg tcgtgcccgc ggtggatctg aaggcggggc ggtgcgtgcg
      781 gctcttgcag ggccgggccg acgccgagac cgtgttctcc gacgatccgg tggcgatggc
      841 gcggcgctgg caggactcgc gcgcaccgcg cctgcacgtg gtggatctcg acggggcctt
      901 cgcgggccgc ccgtcccaga ccgacctcgt ccgcgcaatg atcgccgccg tgcggatccc
      961 ggtgcaggtg ggcggcgggt tgcgcgagat cgcccacgtc gaggcggtgc tggaggcggg
     1021 tgcgcggtgg gccatcgtgg gcacgcgggc cgcgctcgat cccgcgttcc tgggccaggt
     1081 gtgcgagcgg ttcggcgagc aggtcatcgt cggcatcgac gcctccgacg ggcgcgtcgc
     1141 ggtagacggc tggacgcgcg tcctcgacct cgacgccatc gccctggcgc gcgacgcggc
     1201 ggcggcggga gcggccggca tcatctatac cgacatcgcg cgcgacggca cccagaacgg
     1261 ccccaacctg tggagcaccg aggcggtggc caaggcggcg ggcatcgacg tcttcgcctc
     1321 cggcggggta ggttcgctcg acgacatccg gcagctcgcg acggtgccgg agctggccgg
     1381 cgtcatcgtg gggcgcgcgc tctacagcgg tgcggtggac ctgggcgccg cgctggccgc
     1441 ggtgcggtag gcggccatgc tctgcaagcg gctgatcccg tgcctcgacg tcaaggacgg
     1501 acgtgtggtg aagggtgtcc gcttcgtcga gctgcgcgac gcgggcgatc cggtggccgc
     1561 cgcgctggcc tacgacgccc agggcgccga cgagctggtg ttcctcgaca tcaccgcctc
     1621 gcacgagggg cgcgccacca tgctcgacgt ggtccggcgc accgccgagg gcatctacat
     1681 gccgctgacg gtgggcggcg gggtccggtc gctcgaggac atccgcacgc tcctgcgcgc
     1741 cggcgccgac aaggtgtcgc tcaataccgc ggcgctggag cggccgggcc tggtctcgga
     1801 agccgcgcac gccttcggga gccagtgcat cgtggtggcg atagacgcca agtcctccaa
     1861 ccgccactgg gaggtcttca cccacggcgg tcgccgcccc gccgggcgcg acgcggtgga
     1921 gtgggcgcgc gaggtggaga agctgggggc cggcgagatc ctgctcacca gcatggaccg
     1981 cgacggcacc ggcgacggct acgacatcgc gctgacccgc gcggtggcgg aggcggtgtc
     2041 ggtgccggtc atcgcctcgg gcggcgtcgg gcggctcgag catctccgcg agggcctggt
     2101 cgagggccgc gcggatgccg cgctcgtggc gacgctcttt cacttcggcc agcacaccgt
     2161 accggaggcc aagcgctatc tgcacgcccg cggggtgccg gtgaggctcg agccgtgagc
     2221 ccgacggacg atctcaagtg ggacgcgcag gggctgatcc cggcggtggt ccaggacacc
     2281 gagaccggcg agcttctcat ggtggcatgg atgagcgccg agtcgctcgc cgagacgcgg
     2341 cgcaccggcc tcacccactt ctggtcgcgc tcgcgcaacg cgctctggcg caagggcgag
     2401 acgtcggggc acgtccagca cgtggacggt ctctacgccg attgcgaccg ggacacgctg
     2461 ctggtccagg tgcaccagga gggtgtggcg tgccacaccg gggcgcggag ctgcttcttc
     2521 acccgcctcg acggtggcga ggcggcgccc gcgtcggggg cggcgggacc cgcgatgctg
     2581 gaagtcctcg agcgggtgct cgagtcgcgg aaggtggcgc cgccccccgg ctcgtacgtg
     2641 gcggggctgt tcgagaaggg ccagccccag atctgccgga agatcggcga ggaggccgcg
     2701 gaggtgatga ccgcggccct gggcggcgag ggtgacgagc gcgtggtctc cgaggtcgcg
     2761 gacctctggt tccacaccat ggtcctgctg tccgcccgcg gcattcccct ccgccgcgtc
     2821 ttccaggagc tcacccgccg ccacaagcag gacaagaagg acacgtgacc ggccaccccc
     2881 tggcgcgcgc cctgctcgca ctcatcctgc tcgcgggctg cgcgacggca acggggcgta
     2941 tcgagaacgg gatgttctac tcggcgaaag gctaccgtgt gacgctgccg cccgccggct
     3001 ggcgggtgga tgccggccag cgggccgacc tcgcgctgcg cggcgaggcg cgcgccggcg
     3061 gcatggtcgt ggacgcgacc tgcagcggcc gcgagtccct gcgcccgctc gacgtgctcg
     3121 cgcgccacct gaccttcggc ctcacccggc gtgacgtgct ggagaacggt acgtcc
//
LOCUS       DASUFA010000181         7166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1166855, whole genome shotgun sequence.
ACCESSION   DASUFA010000181 DASUFA010000000
VERSION     DASUFA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166855"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFX87_06435"
     CDS             complement(<1..527)
                     /locus_tag="VFX87_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4994586.1"
                     /translation="MSAAFDAITLEVLWTRIISVVDEAAKAIVRTSFSTLSNEANDFA
                     CVLTDARGFALAQNSGSIPSFIGTLPATVRHFLNGIGVRNMRPGDVLITNDPWQGTGH
                     MSDVCLVKPIFYQKEGTGSQPILVAFAATTSHMPDIGGRIRAIEARELFEEGFHIPLT
                     HLIRAGQVDETLLGLL"
     gene            complement(524..2617)
                     /locus_tag="VFX87_06440"
     CDS             complement(524..2617)
                     /locus_tag="VFX87_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4994587.1"
                     /translation="MAQYSLGIDIGGTFTDLVAYDHDAGTSVSRKVLTTHDDPARAVA
                     AGVAGLLAQARLDPGSFSRTVHATTLFTNALIERKGAVTGLITTAGFADTLEIGRERK
                     YELYDLAIAKPEPLVPRHLRLEVAERTRADGRIARRLDTRMLAARADALAKAGVEAIA
                     VVFLHAYANPRHEAEAARLIAKRHPGIAVTTSHEVAPEIREYERASTTAANAYIKPLA
                     QRYLALMARRVSELGIPAPLLLMLSSGGLTHVQEAQRVPVQMLESGPAAGAIAAAFFG
                     QPDSEGDVLAFDMGGTTAKLSLVDGGEPLTAYSFEAARQRRFIEGSGLPIRISTIELI
                     EIGAGGGSIAAVDEIGLLKVGPRSAGSHPGPASYGLGGREPTVTDADFLLGYLNPDYF
                     AGGEVAVDMPAALAAVEGLSRRLGLESTKVAWGIHDIVNENMAGAARVHIAERGRDPR
                     DYALLCTGGAGPVHAFSVARKLGIGRVICPPSAGVASALGLLVAPARVDRVATVGLRL
                     DQGSIAQLESIFARLETEARAVMAETGLKLESATVLRLADGRFLGQGFDLVVPLPPGP
                     YEDSGPGRAVLRGAFETAYREKFALTPPGVPVEFINIRVAVRAPVSGSDVVLEGRRGG
                     GARAALKGTRRAYFPGAEGWAETAVYDRYRLGVGDELAGPAVVEEEGSTLVVGPGATA
                     RVAASGNIVMTLGQP"
     gene            complement(2631..4193)
                     /locus_tag="VFX87_06445"
     CDS             complement(2631..4193)
                     /locus_tag="VFX87_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994588.1"
                     /translation="MTTLRSRRIAPLAVLAATLLLVGFTVAVRPAPASAAEGEMRWGL
                     HVTLAAKWLDPAETEAFNTPFMVLYAVHDALVKPMPGGLSQPSLAESWQESKDRLTYT
                     FVIRKNARFHNGEPVTAEDVKFSFERYKGASNALLKDKVKEVQVLAPNQVRFVLKEPW
                     GDFMTFYGTTASGAAWIVPKKYVQQVGEDGFKTAPVGAGPYKVVSFKPGVEIVMEAFD
                     GYWRKAPSVKRLVMRSMPEETTRAAALRNGEVDIVYLLTGPTAEAIQKMAGFKVVAPL
                     LSGAFWLELPDQWDPKSPWHDRRVRLAASHAIDRQAVNQAETLGFSRLTGSIVPRIFQ
                     YAVPYEPPAYDPARAKKLLAEAGYPNGFDGGDFSPFPPYNSMGEAIQGYLQTVGIRTR
                     MRSMERAAYFSAWREKKLHGVILVITATFGNAATRLEPYATKNGIYAYGSLPEVDDLY
                     VRQGRELDPKKREALVGQIQKTLHDQVTTVPIYELAFIWGVGPRVAESGANLIPGFAY
                     SAPMEDLKLKAR"
     gene            complement(4331..5236)
                     /locus_tag="VFX87_06450"
     CDS             complement(4331..5236)
                     /locus_tag="VFX87_06450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4994589.1"
                     /translation="MADAALPTRDRVEGYFKELNNWGRWGHDDQLGTVNLITPAKQAQ
                     AQRLIKKGRTVSLARDVIPRPVYMYHTTFPSKRERVDVVLDRFDMVYHGFWITHVDAL
                     CHVGWDGELYNGRSFAESLTTEGALWCPIDPIFERGITTRGVFLDIAAGRKEGYVTVG
                     HPVTPRELDEAERRAGVKVEPGDVVVVRSGDERFRVENPDWKPRVSEHPGLHLSCLEW
                     FRAKDIAGISWDMMDERPSRYPGFGMSAHLAIPFLGLALIDNSDPERLAKACAEEGRN
                     EFLFTATPLRLVGATGAPAHPLAIF"
     gene            complement(5259..5531)
                     /locus_tag="VFX87_06455"
     CDS             complement(5259..5531)
                     /locus_tag="VFX87_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994590.1"
                     /translation="MTPQALAAPPVRTVRDVRGAGLAGIVAPYLAVLAWSPPASAVWS
                     AGWRVAAFVDPAASRPAGYLAGSLRPPVLLLLIASSALGFRFGARR"
     gene            complement(5605..6789)
                     /locus_tag="VFX87_06460"
     CDS             complement(5605..6789)
                     /locus_tag="VFX87_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX4994591.1"
                     /translation="MNRRAFVKGAGAVAVVAAGGLVWRAYDQGVFSPGAGAAYEPWTT
                     WRSDAAPGPLGLVRAGILAANPHNTQPWLFQVGAARIELFADTRRNLGAFDPYLREMH
                     IGLGCALENMTRTARVLGYDVAVALHPGPLAAAAASPRPQPVATLALSPARPRPDQLV
                     EAIPRRHTDRGAYAAGRPVPGEMLEAMWAAFQDDADVRLFLFSAPAERKQFGELVQAS
                     TETIIADPAMVADSDRWFRHRWSDVQRRRDGLTLDAAALGPLVTVMGKLMPPASAETS
                     HRYWRDATRDVQVPSSALFGLIAVRDLYDRPQALRVGQRWQHLHLMATARGLAMQPVN
                     QPVEMVDRERELGQAPRAALALAALTGEAGWKPTFAFRAGVPTRPAGPSPRRPVDAVV
                     LA"
     gene            6902..>7166
                     /locus_tag="VFX87_06465"
     CDS             6902..>7166
                     /locus_tag="VFX87_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994592.1"
                     /translation="MDLNYTAEDVAFRKRVRTWLKQNMPRHIKNLEERKAWHRKLYEA
                     GYLGMGWPREYGGQDSRPMEQAIVADEMARAGAPAPTNGLGVGI"
BASE COUNT         1035 a         2568 c         2593 g          970 t
ORIGIN      
        1 aggaggccca gcagggtctc gtccacctgc cccgcgcgga tcaggtgggt gagcgggatg
       61 tggaagccct cctcgaacag ctcgcgcgcc tcgatggcgc ggatgcggcc gccgatgtcc
      121 ggcatgtgtg aagtggtcgc ggcaaaggcg accagtatcg gttgactgcc ggtgccctcc
      181 ttctgataga agatgggctt caccaggcag acgtcgctca tgtggccggt gccctgccac
      241 gggtcgttgg tgatcagcac gtcgccgggc cgcatgttcc ggacgccgat cccattcagg
      301 aaatgccgga ccgtcgcggg cagcgtgccg atgaaggatg ggatgctgcc ggagttctgg
      361 gcgagcgcga agccgcgggc gtccgtgagc acgcacgcga agtcgttcgc ctcgttcgac
      421 agcgtggaga acgaggtgcg cacgatcgcc ttcgcggcct cgtccaccac cgagatgatc
      481 cgcgtccaca gcacctccag cgtgatcgcg tcgaacgccg cgctcatggc tgccccaggg
      541 tcatcacgat gttgccgctc gcggccacgc gcgcggtcgc gccgggcccg accacgagcg
      601 tggagccttc ttcctccacc accgcgggcc cggccagctc gtcgccgaca ccgaggcggt
      661 agcgatcgta caccgccgtc tccgcccagc cttccgcgcc cgggaagtac gcccgccgcg
      721 tgcccttcag cgcggcgcgc gcgccaccgc cgcgccggcc ctcgagcacg acgtcgctgc
      781 ccgacacggg cgcccgcacc gccacgcgga tgttgatgaa ctccaccggc acgcccggcg
      841 gcgtcagcgc gaacttctcg cgatacgcgg tctcgaacgc gccgcggagc accgcgcggc
      901 cgggcccgct gtcctcgtag ggaccgggcg gcagcggcac caccaggtcg aagccctggc
      961 cgaggaaccg gccgtcggcc aggcgcagca cggtggcgct ctcgagcttg agcccggtct
     1021 ccgccatcac cgcccgcgcc tcggtttcca gacgggcgaa gatggactcg agctgcgcga
     1081 tgctgccctg gtcgagccgg aggcccacgg tggccacccg gtccacgcgc gccggggcca
     1141 ccagcaggcc gagcgcggag gccaccccgg ccgacggcgg gcagatcacg cggccgatcc
     1201 cgagcttgcg ggccaccgag aaggcgtgca ccggacccgc gccgcccgtg cacaggagcg
     1261 cgtagtcgcg cggatcgcgc ccgcgctccg cgatgtgcac ccgcgcggcg cccgccatgt
     1321 tctcgttgac gatgtcgtgg atgccccatg cgaccttcgt ggactcgagc ccgagccgcc
     1381 gcgacagccc ctcgaccgcg gccagcgccg ccggcatgtc caccgccacc tcgccgcccg
     1441 cgaagtagtc cgggttgaga tagcccagca ggaagtcggc gtcggtcacc gtgggctcgc
     1501 ggccgcccag gccgtaggag gccgggccgg gatgcgaccc cgcgctgcgc gggccgacct
     1561 tgagcagccc gatctcgtcc accgccgcga tgctgccgcc gcccgcgccg atctcgatca
     1621 gctcgatggt ggagatgcgg atgggcagcc cgctgccctc gatgaagcgc cgctgccgcg
     1681 cggcctcgaa gctgtacgcg gtgagcggct cgcccccgtc caccagcgag agcttggcgg
     1741 tggtgccgcc catgtcgaag gcaagcacgt caccctcgct gtccggctgg ccgaagaagg
     1801 cggccgcgat ggcgccggcg gccggccccg actccagcat ctgcaccggc acccgctgcg
     1861 cctcctgcac gtgggtcagc ccgccgctcg acagcatcag caggagcggc gccggaatgc
     1921 ccagctccga cacgcgccgg gccatcagcg cgaggtacct ctgcgccagc ggcttgatgt
     1981 aggcgttggc cgcggtggtg gaggcgcgct cgtactcgcg gatctccggc gccacctcgt
     2041 gcgaggtcgt caccgcgatg ccgggatgcc gcttcgcgat cagccgcgcc gcctccgcct
     2101 cgtgccgcgg attcgcgtag gcgtgcagga acaccaccgc gatggcctcg acgcccgcct
     2161 tcgccagcgc gtcggcccgc gcggccagca tccgcgtgtc caggcgccgc gcgatccggc
     2221 cgtcggcccg cgtgcgctcg gccacctcga gccgcaggtg acggggcacg agcggctcgg
     2281 gtttggcgat cgccagatcg tagagctcgt acttgcgctc gcgcccgatc tcgagcgtgt
     2341 ccgcgaagcc cgcggtggtg atgagaccgg tcaccgcgcc cttgcgctcg atgagcgcgt
     2401 tggtgaagag cgtggtggcg tggacggtcc gggagaacga gcccgggtcg aggcgcgcct
     2461 gcgcgagcag acccgcgacg ccggcggcga ccgcgcgcgc cggatcgtcg tgggtcgtga
     2521 ggaccttgcg gctcacgctc gtgcccgcgt cgtggtcgta cgccaccagg tcggtgaagg
     2581 tcccgccgat gtcgatcccg agcgagtact gggccaccgg gccggctcct ctaccgggcc
     2641 ttcagcttga gatcctccat cggcgcggag taggcgaagc cggggatcag gttcgccccg
     2701 ctctccgcca cccgcggacc gaccccccag atgaaggcca gctcatagat cgggacggtc
     2761 gtgacctggt cgtggagcgt cttctggatc tgcccgacca gcgcctcgcg cttcttcggg
     2821 tccagctcgc gcccctggcg tacgtagaga tcgtccacct cgggaaggct gccgtaggcg
     2881 tagatgccgt tcttggtggc gtagggctcg agccgcgtgg ccgcgttgcc gaaggtggcg
     2941 gtgatcacga ggatcacccc gtggagcttc ttctcccgcc aggccgagaa gtaggccgcg
     3001 cgctccatgc tgcgcatcct ggtgcggatg cccaccgtct gcaggtagcc ctggatggcc
     3061 tcgcccatgg agttgtaggg ggggaacggc gagaagtcgc cgccgtcgaa gccgttgggg
     3121 tagccggcct cggcgaggag cttcttggcc cgcgccggat cgtaggccgg cggctcgtag
     3181 ggcaccgcgt actggaagat gcgcggcacg atgctgccgg tgagccgcga gaagccgagc
     3241 gtctccgcct ggttcaccgc ctgccggtcg atggcgtggc tggccgccag gcgcacgcgg
     3301 cggtcgtgcc acggcgactt ggggtcccac tgatcgggca gctcgagcca gaacgcgccg
     3361 gacagcagcg gggccaccac cttgaagccc gccatcttct ggatggcctc cgccgtcggg
     3421 ccggtgagca ggtagacgat atccacctcg ccgttgcgaa gcgccgccgc ccgcgtggtc
     3481 tcttccggca tcgagcgcat gacgagccgc ttcaccgagg gcgccttgcg ccagtagccg
     3541 tcgaaggcct ccatcacgat ctcgacgccc ggcttgaacg agaccacctt gtacggcccc
     3601 gcgccgaccg gcgcggtctt gaagccgtcc tcgcccacct gctgcacgta cttcttgggc
     3661 acgatccagg cggctccgga ggccgtcgtg ccgtagaacg tcatgaagtc gccccacggc
     3721 tccttgagca cgaagcggac ttgattgggc gccagcacct gcacttcctt gaccttgtcc
     3781 ttcaagaggg cgttgctcgc gcctttgtaa cgctcgaagg agaactttac gtcctcggcg
     3841 gtgaccggct cgccgttgtg gaagcgggcg ttcttccgga tcacgaacgt gtaggtgagc
     3901 cggtccttcg actcctgcca cgactcggcg aggctcggct ggctgagccc gcccggcatc
     3961 ggcttcacga gcgcgtcgtg caccgcgtag agcaccatga acggcgtgtt gaacgcttcg
     4021 gtctcggcgg gatcgagcca cttggcggcc agggtgacgt ggaggcccca gcgcatctcg
     4081 ccctcggccg cggaggcggg cgcgggacga accgcgacgg tgaagccgac caggagcagc
     4141 gttgccgcga gcacggcgag cggagcgatc cggcgcgaac gaagtgtggt catggcgaga
     4201 acctagggcc cgcggcgcgt cacgtcaagt gggggtgtcg acggcgcggg cgaggcggct
     4261 cgccccgggt ctcgagagcg acctggccgc ccggcgcgct gaccggccgg gccggcaccc
     4321 cgcgccgcga tcagaagatc gcgagcgggt gcgccggcgc gccggtggcg ccgaccaggc
     4381 gcagcggcgt cgcggtgaag aggaactcgt tgcgcccttc ctccgcgcac gccttggcga
     4441 ggcgctccgg atccgagttg tcgatcagcg ccagtcccag gaacgggatg gccaggtgcg
     4501 ccgacatgcc gaagcccggg tagcgactgg ggcgctcgtc catcatgtcc caggagatgc
     4561 ccgcgatgtc cttcgcgcgg aaccactcga ggcaggagag atggagaccg gggtgctcgg
     4621 agacccgcgg cttccagtcg gggttctcga cccggaagcg ctcgtcgccg ctgcgcacca
     4681 ccaccacgtc gcccggctcc accttcacgc cggcgcgccg ctccgcctca tcgagctctc
     4741 tgggtgtcac gggatggccc accgtgacgt agccttcctt gcgcccggcc gcgatgtcga
     4801 ggaagacccc gcgggtggtg atgccgcgtt cgaagatcgg atcgatcggg caccagagcg
     4861 cgccctcggt ggtgaggctc tccgcgaatg accggccgtt gtagagctca ccatcccagc
     4921 ccacgtgaca gagcgcgtcc acgtgggtga tccagaagcc gtggtagacc atgtcgaagc
     4981 ggtcgagcac gacgtccacg cgctcgcgct tggatggaaa cgtcgtgtgg tacatgtaca
     5041 cgggccgcgg gatcacgtcg cgcgccagcg acacggtgcg gcccttcttg atcaggcgct
     5101 gcgcctgcgc ctgcttggcg ggggtgatca ggttcacggt gccgagctgg tcgtcgtggc
     5161 cccagcggcc ccagttgttc agctccttga agtacccctc gacacgatcg cgggtgggca
     5221 gggcggcatc ggccatggga cggatccttt cctggcggct agcggcgcgc gccgaagcgg
     5281 aagccgagcg ccgacgaggc gatgagcagc agaaggacgg gcggccggag cgaaccggcg
     5341 aggtagcccg cggggcgcga cgcggcggga tccacgaagg cggccacgcg ccacccggcg
     5401 ctccagaccg cggacgccgg cggggaccag gcgaggacgg cgagatacgg cgcgacgatt
     5461 cccgcgagcc cggccccccg cacgtcgcga acggtgcgca ccggcggggc ggccagggcc
     5521 tgcggcgtca tggccgaaaa ggtaacacga tcccggcatc atgacgtgcc ggaagctcga
     5581 agcgacggag atgatccgga aggatcaggc gagcacgacc gcgtccaccg gacgccgcgg
     5641 gctgggcccg gcggggcgcg tgggcacccc cgcgcggaac gcgaaggtgg gcttccaccc
     5701 ggcctcgccg gtcagcgcgg ccagcgccag ggccgcgcgc ggcgcctgcc ccagctcgcg
     5761 ctcccggtcc accatctcca ccggctggtt gaccggctgc atcgcgagcc cgcgcgccgt
     5821 ggccatgagg tgcaggtgct gccaccgctg ccccacgcgc agggcctgcg ggcgatcgta
     5881 gagatcgcgc accgcgatca ggccgaagag cgcgctgctg ggcacctgga cgtcgcgggt
     5941 ggcgtcgcgc cagtagcggt ggctcgtctc ggcggaggcc ggcggcatca gcttgcccat
     6001 cacggtgacg agcgggccga gcgcggccgc gtccagcgtg agcccgtcgc gccgccgctg
     6061 cacatcggac cagcgatggc ggaaccaccg gtcgctgtcc gccaccatcg cggggtccgc
     6121 gatgatcgtc tcggtggatg cctgcaccag ctcgccgaac tgcttgcgct cggccggcgc
     6181 cgagaacagg aagagacgca cgtccgcgtc gtcctggaag gcggcccaca tcgcctccag
     6241 catctcgccc ggcaccgggc gcccggccgc gtaggcgccg cgatcggtgt gccggcgcgg
     6301 gatggcctcc acgagctggt ccgggcgcgg ccgcgcgggc gagagcgcga gcgtcgccac
     6361 cggctgcggg cggggcgacg cggcggccgc cgcgagcggg cccggatgaa gggcgaccgc
     6421 gacgtcgtag cccaggacgc gggcggtccg cgtcatgttc tcgagcgcgc agccgaggcc
     6481 gatgtgcatc tcgcgcaggt acgggtcgaa ggcgccgagg ttgcgtcggg tgtcggcgaa
     6541 gagctcgatc cgggccgcgc ccacctggaa gagccacggc tgggtgttgt ggggattggc
     6601 ggcgaggata ccggcccgca ccaggccgag cggccccggc gccgcgtcgc tccgccaggt
     6661 cgtccacggc tcgtaggccg cgccggcccc gggcgagaag acgccctgat cgtacgcgcg
     6721 ccagaccagc cccccggcgg ccacgaccgc gacggctccc gcgcccttca cgaaggctcg
     6781 gcggttcacg cgaggaaaag gtagcacggg ggcccggttg accgtcgcgc ggggcccgtg
     6841 ctacaagcgg ggctaccccc aacggcagcg ccagccgcag gcccggcaag gaggcccccc
     6901 aatggatctc aactacaccg ccgaggacgt cgccttccgc aagcgggtcc gcacgtggct
     6961 caagcagaac atgccgcggc acatcaagaa cctcgaggag cgcaaggcct ggcaccgcaa
     7021 gctgtacgag gccggttacc tcggcatggg gtggccccgg gagtacggcg gccaggactc
     7081 gcggccgatg gagcaggcga tcgtggccga cgagatggcg cgcgcgggcg cgcccgcccc
     7141 gaccaacggg ctcggcgtcg gcatcg
//
LOCUS       DASUFA010000182         9507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_257515, whole genome shotgun sequence.
ACCESSION   DASUFA010000182 DASUFA010000000
VERSION     DASUFA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257515"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VFX87_06470"
     CDS             complement(<1..1165)
                     /locus_tag="VFX87_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4994593.1"
                     /translation="MDALKQRIAESVDGLGDELAALSSRIHAHPELAFEERRACGWIG
                     EFLAGHGFQVEAGVGGVETAFRATLETGPGPTLAILCEYDALPGIGHACGHNVIAAAG
                     AGAGAALMAVRDRLPGGRIQVIGTPAEERGGGKGVLVDAGVFKDVAGAMMIHGFDRTL
                     LHQDLLGVVRVTFEWAGRAAHASTDPWEGTNALDACVETFNAVAMLRQQMRPDCRIHG
                     VVAEGGAAANIIPERAVADFNVRGPSLEAMWALYRRVVAAAEGAAAATSTRLTAFQHP
                     DVYEPLRRNQALNDVFAGNLAAAGLAEGPAAPDRVASSDIGNVSQVTPTIHAWVAIAP
                     LGTAIHTREFAAAATAPAARAGLLAGAKLMALSVADLLADPSRLAAMKEEFRRR"
     gene            1190..1906
                     /gene="mtgA"
                     /locus_tag="VFX87_06475"
     CDS             1190..1906
                     /gene="mtgA"
                     /locus_tag="VFX87_06475"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX4994594.1"
                     /translation="MRRRRGWAGRLVALVLLAAAIWIGVEIWRWPAVGRLATERPATT
                     AFIERYRAQQRATGRPGRVAWTWTSYSAISPALKRAVLVGEDIGFFSHHGFALAEMQS
                     ALEDALRDGELPRGASTITQQLAKNLWLSPSRNPLRKAREALLTWQLERALSKRRILE
                     LYLNVVELGPGIYGVGAASQRYFGKAPADLDESEAAQLAAALPNPSAWHPGATSAAYR
                     RHVATIERRMNRAAFLFKQI"
     gene            complement(1925..2563)
                     /locus_tag="VFX87_06480"
     CDS             complement(1925..2563)
                     /locus_tag="VFX87_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX4994595.1"
                     /translation="MKVEAVIFDMDGVLVDSEPFGFEALRRVMARYGLGYSEEENAEF
                     LGRTTLESCRILKARHALPDAAETLADFYIEGMLAQIGDGPIPMPGVPDVLERVRAGG
                     YRLALASSAEVRVIQANLTALALGPLFEAVVSGTQVARGKPAPDVFLAAADRLAVAAD
                     RCLVIEDSRNGLLAAKAAGMTCAVVPCAHTRHQDFREADHRLAALADLLTLL"
     gene            complement(2560..3498)
                     /locus_tag="VFX87_06485"
     CDS             complement(2560..3498)
                     /locus_tag="VFX87_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX4994596.1"
                     /translation="MSERDWRWERQLHGIVPPLISPLDDAGAPDAGALAALVDHVIGG
                     GCSGLFMLGGCGEGAWLTSAQRGAVVRAAARAAASRVPVLAGVMLPATGPAIEAARQA
                     AGEGADALVLGSPYYFGVEPAAQERHVEAVLAATPLPGLLYNIPQCTHHVLTADTVGR
                     LARDPRVLGIKDSAGDFEAFQKLLAIKREHAPFRVLQGHESLAAASLLQGGDGMVPGM
                     ANLAPALFVRLQAAADRGAAAECAELQRQVTAIARIYQQGPWLSALKAACAMLGLGNG
                     RPAPPLAPAVPAARAAIAALLDEAGLGTATGAARSR"
     gene            3569..4219
                     /gene="msrA"
                     /locus_tag="VFX87_06490"
     CDS             3569..4219
                     /gene="msrA"
                     /locus_tag="VFX87_06490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX4994597.1"
                     /translation="MFGSRKRGLPSAAEALPGRPERMPVPETHFVTGARLEPPFPAGT
                     ERAVFGMGCFWGEERKFWGIPGVYSTSVGYAGGHTPNPTYEEVCSGATGHAEVVLVVF
                     DPAKVSYETLLRVFWENHDPTQGMRQGNDVGTQYRSAIYCYGPAQQEAAEASRKAYQA
                     VLEKAGYGPITTEIRQAPEYYYAEDYHQQYLAKNPWGYCGIGGTGVACPTGLVQST"
     gene            4236..4811
                     /locus_tag="VFX87_06495"
     CDS             4236..4811
                     /locus_tag="VFX87_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994598.1"
                     /translation="MPVLARTAVAALLAALLGGCALASSIGTTTPTGIGQQLLIRSLE
                     RALSDLDLARLRSETVALEISVHSGNEAFVKDFVATWMKAHGVRVGGETPARKLKMFV
                     SVYGTDRDQTLIGVPAFQAPVVNVPIPELAFFKWVRNRGQAELRLWAFDAKGDTLVDA
                     PAPGIGRAKYDDFTVLLFIGFTRSDVDQRPN"
     gene            4814..6025
                     /locus_tag="VFX87_06500"
     CDS             4814..6025
                     /locus_tag="VFX87_06500"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994599.1"
                     /translation="MADDSLPLHGLLVVDLTRVLAGPYCTRLLSDLGARVIKIERPIE
                     GDEMRHNPHQIEPGRDDQSTYFARVNAGKESVAVDLSRPEGRDVVLELAGHADVFIEN
                     FAPGVAGKLGLAYDAVRAAKPDIVYCSISGFGQTGPWRERPAFAHIINAASGMMALEQ
                     GDEAAPRASNLQAADVLAAAHAMGAIMAALWRRARTGQGAHLDVSMLEALVGADSVTY
                     ASVLNGGEEHGNPRPGMIVHKIGDRYMAMQIVGAPRLWQRLLGIMKRPELGEDPRFAT
                     ALTRRRNWRELRGIITGWLDTFPGADAALAVLGDARIPCAPVLRPAEVIATEHLATRG
                     FFPVLPHPAMPSLRVTASPYHLDGRPTHPRAPAAYHVGQHTRGVLRDLLGYKDERIAA
                     LIAAGIVEGPA"
     gene            6022..6327
                     /locus_tag="VFX87_06505"
     CDS             6022..6327
                     /locus_tag="VFX87_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994600.1"
                     /translation="MNYREEALQRTEKEIVAEKAATLGRAGERLEAALRLAALLHDAV
                     REAPEGEARERARERYRQARTRALEARRILIIQREAIGLRNHRLVDQQFPEPPRLDV"
     gene            6463..6813
                     /locus_tag="VFX87_06510"
     CDS             6463..6813
                     /locus_tag="VFX87_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994601.1"
                     /translation="MIRATVLTCTVLLCSGFAWPPDYAEQSRADVTTCVSYAQRTSPG
                     FAAWVRGVDLQTGHVDIERASIEDSRGERAFSRCLIAMRHWRLIERNLPKLTEPGEPD
                     PTTMADGGSDSLTR"
     gene            6881..7801
                     /locus_tag="VFX87_06515"
     CDS             6881..7801
                     /locus_tag="VFX87_06515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4994602.1"
                     /translation="MKLGICLPHYGRPIEPSRLAQIAARAEEMSLDSIWVTDHVIVPR
                     DVPLIYRSDMLDPLAMLPWLAGVTERIALGTSVIVLPYRSPLPVAKLLASVDVLSGGR
                     LIVGVAVGWVEGEFEALNVPFRERGRRTDEAIDLFRTVWTHEYPEIKTANHTLTNVKA
                     SPMPLQKPRPPILVGGASEAAMRRAARLGDGWHASGMPSSMFRAGALAVANFWKEAKR
                     EGNPQLSLRIPILIDGVHQAAVDTALLGTRHVLRGPLTNIVRELRQYQTAGCEHVALE
                     VSYSTYPAILETIDIIAEEIRPALEQQRER"
     gene            complement(7991..9076)
                     /locus_tag="VFX87_06520"
     CDS             complement(7991..9076)
                     /locus_tag="VFX87_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX4994603.1"
                     /translation="MRLREVRADHYRIPLPVPLSDSTHGVIEGFELVTVRLRDEDGAE
                     GVGYTYTVGTGGSAVHAVLSRDLALRLTGREAERIEELWQAMWWALHYGGRGGAQGLA
                     ISAVDIALWDLRARRQGAPLWRVLGGFDPRVPCYAGGIDLEFPLDALLRQTDDNLARG
                     FRAIKMKVGRSSLLEDVERVRAMRGHLGPEFPLMVDANMRWSVDDAIRAARALQDLNP
                     VWLEEPTIPDDVPGHARIVREGGLPIAAGENLHTLYEFRALIAAGGVTFPEPDVTNCG
                     GITSFMKICHLAEAFNLPVTSHGAHDITVHLLGAVPNRSYLEAHGFGLDRYIAEPLRL
                     EDGRAVAPDRPGHGIAFDWQGLEKRRV"
     gene            complement(9076..>9507)
                     /locus_tag="VFX87_06525"
     CDS             complement(9076..>9507)
                     /locus_tag="VFX87_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4994604.1"
                     /translation="DAKLARARELGAAGGANYRTQDWAKEILALTGGRGPDVVIDSVG
                     GETFARAVDVLRPGGRIATYGATTGPVRELVLRAVFWRQISVLGTTMGSPAEFGAMLE
                     LYARDGLRPAVDRIFPLAEAAAAHRHMEEAGQFGKIVLATA"
BASE COUNT         1370 a         3551 c         3335 g         1251 t
ORIGIN      
        1 accgtcgcct gaactcctcc ttcatcgcgg cgagccggga tggatcggcg agcaggtccg
       61 ccacgctgag cgccatcagc ttggcgcccg cgagcagccc cgcccgtgcg gccggcgccg
      121 tcgcggcggc ggcgaactca cgggtgtgga tcgcggtccc caggggcgcg atggccaccc
      181 aggcgtggat cgtcggggtg acctggctca cgttgccgat gtccgaggag gcgacacgat
      241 ccggcgcggc cgggccctcg gcgagcccgg cggcggccag gttgcccgcg aagacgtcgt
      301 tgagcgcctg gttgcgccgg agcggctcgt agacgtcggg atgctggaag gcggtgagac
      361 gggtggacgt ggcggcggcc gcgccctccg cagcagccac cacgcggcgg tagagcgccc
      421 acatcgcctc gaggctcggc ccgcgcacat tgaagtcggc gaccgcgcgc tcggggatga
      481 tgttggcggc ggcgccgccc tccgcgacga cgccgtggat gcggcaatcg gggcgcatct
      541 gctggcgaag catggccacc gcgttgaagg tctcgacgca ggcgtcgagc gcgttcgtgc
      601 cctcccacgg atccgtcgaa gcgtgggcgg cgcggcccgc ccactcgaac gtcacgcgca
      661 cgacgccgag caggtcctgg tggagcaggg tgcgatcgaa gccgtggatc atcatcgcgc
      721 cggccacgtc cttgaagacg cccgcgtcca cgagcacgcc cttgcccccg ccccgctcct
      781 cggcgggcgt gccgatcacc tggatgcgcc cgccgggcag gcggtcgcgc accgccatca
      841 gcgcggcgcc cgcgccggcc cccgcggcgg cgatgacgtt gtggccgcag gcgtggccga
      901 tgcccggcag cgcgtcgtac tcgcagagga tggcgagcgt cggccccggg cccgtctcga
      961 gggtcgcgcg aaacgcggtc tcgacaccgc ccaccccggc ctccacctgg aagccgtgcc
     1021 cggcgaggaa ctcgccgatc cagccgcagg cgcgccgctc ctcgaaggcc agctccggat
     1081 gcgcgtggat gcgggacgac agcgcggcga gctcgtcgcc cagcccgtcc accgactccg
     1141 cgatgcgttg cttgagggcg tccatcggcc caccacgata ccatgagcca tgcgcaggcg
     1201 acggggatgg gcgggccggc tcgtcgcgct cgtactgctg gcggccgcga tctggatcgg
     1261 cgtcgagatc tggcggtggc ccgcggtggg acggctcgcc accgagcgcc ccgcgaccac
     1321 cgcgttcatc gagcggtatc gcgcccagca gcgcgcgacc ggccgaccgg gccgcgtcgc
     1381 ctggacctgg acgtcctatt cggcgatctc gcccgcgctc aagcgcgcgg tgttggtcgg
     1441 cgaggacatc ggtttctttt cccaccacgg cttcgcgctc gccgagatgc agagcgcgct
     1501 cgaggacgcg ctgcgggacg gcgagctgcc gcgcggagcg tccaccatca cccagcagct
     1561 cgccaagaac ctctggctct ccccgtcacg caacccgctg cgcaaggcga gggaggcgct
     1621 cctgacctgg cagctcgagc gcgcgctctc caagcggcgc atcctggagc tgtatctcaa
     1681 cgtggtcgag ctcgggccgg gcatctacgg ggtgggcgcc gcgagccagc gctacttcgg
     1741 caaggcgccc gccgatctcg acgagagcga ggcggcccag cttgccgcgg cgctgccgaa
     1801 tccgtccgcc tggcatcccg gcgcgaccag cgcggcctac cggcgccacg tggcgaccat
     1861 cgagcggcgg atgaaccgcg ccgccttcct cttcaagcag atctagccgg ccgccggcag
     1921 gaagtcagag gagcgtgagg agatcagcca gcgcggccag gcggtgatcc gcctcgcgaa
     1981 aatcctggtg gcgggtgtgg gcgcagggca ccaccgcgca ggtcatgccc gcggccttgg
     2041 ccgcgagcag gccgttgcgc gagtcctcga tgaccaggca gcgatcggcc gccaccgcga
     2101 gccggtcggc cgcggcgagg aacacgtcgg gggcgggctt gccccgcgcg acctgggtgc
     2161 cggagaccac cgcctcgaag agcggtccca gcgcgagcgc ggtgaggttg gcctggatca
     2221 cccgcacctc cgccgacgag gccagggcca gccggtatcc cccggcccgc acccgctcca
     2281 gcacgtcggg cactcccggc atcggaatcg ggccgtctcc gatctgcgcg agcatgccct
     2341 cgatgtagaa gtcggccagc gtctccgcgg catccggcag cgcgtggcgg gccttcagga
     2401 tgcggcacga ctcgagcgtg gtgcggccga ggaactccgc gttctcttcc tccgagtacc
     2461 cgagcccgta gcgggccatc acccggcgca gggcctcgaa accgaacggc tccgagtcca
     2521 cgagcacacc gtccatgtcg aagatgacgg cctcgacctt caccgggagc gcgcggctcc
     2581 cgtggccgta cccagccccg cctcgtccag gagggcggcg atcgccgcgc gcgcggcggg
     2641 cacggccggc gcgagtggcg gcgccgggcg gccattgccc agccccagca tggcgcacgc
     2701 cgccttgagg gccgacagcc acggcccctg ctggtagatc cgcgcgatcg cggtcacctg
     2761 ccgctggagc tccgcgcact ccgccgccgc gccgcggtcg gcggcggcct gcaggcggac
     2821 gaagagcgcg ggggcgagat tggccatgcc cggcaccatg ccgtcgccgc cctgcagcag
     2881 gctcgccgcg gccagcgact cgtggccctg cagcacccgg aagggcgcgt gctcccgctt
     2941 gatcgcgagc agcttctgga aggcctcgaa gtcgcccgcc gagtccttga tgccgagcac
     3001 ccgcggatcc cgggccagcc ggcccaccgt gtcggcggtc agcacgtggt gcgtgcactg
     3061 ggggatgttg tagagcagcc cgggcagcgg cgtggcggcc agcaccgctt ccacgtgacg
     3121 ctcctgcgcg gcgggctcca ccccgaagta gtacggagag ccgagcacga gtgcgtccgc
     3181 accctcgccc gcggcctggc gggcggcctc gatggcgggc ccggtggcgg gcagcatcac
     3241 gccggccagc accggcacgc ggctggcggc cgcgcgcgcc gccgcccgca ccacggcgcc
     3301 gcgctgcgcg gaggtgagcc aggccccctc gccgcagccg cccagcatga acaggccgga
     3361 gcagccaccg ccgatcacgt ggtccaccag cgcggccagc gcgcccgcgt ccggcgcgcc
     3421 cgcgtcgtcc agcggcgaga tcagcggcgg aacgatcccg tgaagctgcc gctcccaccg
     3481 ccagtcgcgt tcgctcatgc cgacgagcat agcagagtgg cctcggcggc caccctgatg
     3541 taggctctac cgggaggagg acagccccat gttcggttcg cgaaagcgcg gtttgccgtc
     3601 ggccgccgag gcgctccccg gccgcccgga acgcatgccg gttcccgaga cccacttcgt
     3661 gaccggggcc cgtctcgagc ctcccttccc ggcgggcacc gagcgcgccg tcttcggcat
     3721 gggctgcttc tggggcgagg agcggaagtt ctgggggatc ccgggcgtct actcgacctc
     3781 cgtcggctac gcgggcggcc acacgccgaa cccgacctac gaggaggtct gcagcggcgc
     3841 gaccggccac gccgaggtcg tgctggtcgt gttcgatccc gccaaggtct cgtacgagac
     3901 cctgctacgc gtcttctggg agaaccacga tccgacgcag ggcatgcggc agggcaacga
     3961 cgtcgggacc cagtaccgct cggccatcta ctgctacggg cccgcccagc aggaggcggc
     4021 ggaggcctcg cgcaaggcgt accaggcggt gctcgagaag gcgggctacg gtccgatcac
     4081 gaccgagatc cgccaggctc ccgagtacta ctacgccgag gactatcacc agcagtatct
     4141 agccaagaat ccgtggggct actgcggcat cggcggcacc ggcgtcgcct gtccgacggg
     4201 cctggtgcag tccacctgag gccccgggcc gcccgatgcc cgtcctcgcc cgcaccgcgg
     4261 tcgcggcgct cctcgccgcg ctgctcgggg gctgcgcgct cgccagctcc atcggcacca
     4321 ccacgccgac cgggatcggc cagcagctcc tgatccgctc gctcgagcgc gcgctctccg
     4381 atctcgacct ggcccgcctg cgcagcgaga cggtcgccct ggagatatcg gtccactcgg
     4441 gcaacgaagc cttcgttaag gacttcgtcg ccacctggat gaaggcgcat ggcgtgcgcg
     4501 tcggcggcga gacccctgcg cggaagctca agatgttcgt gtcggtctac ggcaccgacc
     4561 gcgatcagac cctgatcggc gtgcccgcgt tccaggcgcc ggtggtgaac gtgccgatcc
     4621 ccgagctggc cttcttcaaa tgggtgcgca accgcggcca ggccgagctg cgcctgtggg
     4681 cgttcgacgc caagggcgac acgctcgtgg acgcccccgc gcccggcatc ggccgcgcga
     4741 agtacgacga cttcaccgtg ctgctgttca tcggcttcac acgctccgac gtcgaccagc
     4801 gcccgaactg accgtggccg acgattccct tcccctgcac ggcctgctgg tcgtcgacct
     4861 gacccgcgtg ctcgccggcc cctactgcac gcgcctgctc tccgacctgg gcgcccgcgt
     4921 catcaagatc gagcgcccca tcgagggcga cgagatgcgc cacaacccgc accagatcga
     4981 gcccgggcgc gacgaccagt ccacctactt cgcacgcgtg aacgcgggca aggagagcgt
     5041 ggcggtggat ctgagccgtc ccgagggccg cgacgtggtc ctggagctcg cggggcacgc
     5101 cgacgtgttc atcgagaact tcgcgcccgg cgtggccggc aagctcggtc tggcctacga
     5161 cgcggtccgc gccgccaagc ccgatatcgt ctactgctcg atctcgggct tcggccagac
     5221 cgggccctgg cgggagcgcc ccgccttcgc ccacatcatc aacgccgcct ccggcatgat
     5281 ggcgctcgag cagggcgacg aggcggcccc gcgggcctcg aatctccagg ccgcggacgt
     5341 cctggccgcc gcccacgcaa tgggcgcgat catggccgcg ctctggcgcc gggcccgcac
     5401 cgggcagggc gcgcacctcg acgtctcgat gctggaggcg ctcgtgggcg ccgacagcgt
     5461 cacctacgcc tcggtgctca acgggggcga ggagcacggc aacccgcgcc cgggcatgat
     5521 cgtgcacaag atcggcgatc gctacatggc gatgcagatc gtgggggcgc cgcggctctg
     5581 gcagcggctg ctcgggatca tgaagcggcc ggagctcggc gaggatccgc gcttcgccac
     5641 cgcgctcacc cgccggcgga actggcgcga gctgcgcggc atcatcaccg ggtggctcga
     5701 caccttcccc ggcgcggacg cggccctcgc ggtgctcggc gacgcgcgca tcccctgcgc
     5761 gccggtgctg cgcccggccg aggtcatcgc caccgagcac ctggccacgc gcggcttctt
     5821 cccggtgctg ccccatccgg ccatgccctc gctgcgcgtg acggccagcc cgtaccacct
     5881 ggacgggcgg cccacccatc cccgggcgcc ggccgcctat cacgtgggcc agcacacgcg
     5941 cggcgtgctc cgcgacctgc tcggctacaa ggacgagcgc atcgcggcgc tcatcgccgc
     6001 gggcatcgtc gaggggccgg cgtgaactac cgcgaggagg cgctccagcg gaccgagaag
     6061 gagatcgtcg cggagaaggc ggccacgctc ggccgcgcgg gcgagcgcct ggaggccgcc
     6121 ctgcgcctgg ccgccctgct gcacgacgcg gtgcgggaag cccccgaggg cgaagcgcgg
     6181 gagcgcgccc gcgagcgcta ccggcaggca cggacgcggg ccctcgaggc gcgccgcatc
     6241 ctgatcatcc agcgcgaggc gatcggcctt cgcaatcacc gcctcgtgga tcagcaattc
     6301 ccggagccgc cccgcctcga cgtctgatcg agcagaaatt tctcggttgt tctgggcctg
     6361 acgcaacatc gtcgcagtac cgccgaccta accccctgat tctccgacgc tcgtcaatgg
     6421 cctgtctctt gctcccaaga tgcgccgcgg aaggagccag ccatgatccg ggccaccgtt
     6481 ctgacgtgca ccgttttgct atgcagcgga tttgcctggc cgcccgacta cgccgagcag
     6541 tcacgggccg acgtgaccac gtgcgtgagc tacgcgcagc ggacgtcgcc cggcttcgcg
     6601 gcgtgggtgc gcggggtgga cctccagacc ggtcacgtgg acatcgagcg cgcctccatc
     6661 gaggactcgc gaggcgagcg cgcgttctcg cgctgcctga ttgcgatgcg gcactggcga
     6721 ttgatcgagc gcaacctgcc gaagctcacc gagcccggcg agcccgatcc gaccacgatg
     6781 gcggacgggg gcagcgattc gctgacccgc taggccatca cccgcaggtc atcggaggac
     6841 gcgctgctag acgccgccgg ggcgtggtag gctccggccc atgaagctcg ggatctgcct
     6901 gccccactac gggcgcccca tcgagccctc gcggctcgcc cagatcgcgg cccgcgccga
     6961 ggagatgagc ctcgactcga tctgggtgac ggaccacgtc atcgtgccgc gcgacgtgcc
     7021 gctgatctac cgcagcgaca tgctggaccc gctcgcgatg ctgccgtggc tggccggcgt
     7081 caccgagcgc atcgcgctcg gcaccagcgt catcgtgctg ccctaccggt ctcccctgcc
     7141 cgtggccaag ctgctcgcca gcgtggacgt gctctccggc ggccgcctca tcgtgggcgt
     7201 ggcggtgggc tgggtcgagg gcgagttcga ggccctcaac gtccccttcc gcgagcgcgg
     7261 ccgccgcacc gacgaagcca tcgatctctt tcgcacggtg tggacccacg aatatcctga
     7321 aatcaagacc gccaaccaca ccctcaccaa cgtgaaggcc tcgccgatgc cgctacagaa
     7381 gccccgccca cccatcctgg tcggcggcgc ctccgaagcg gccatgcgcc gcgccgcccg
     7441 cctgggcgac ggctggcacg cctcaggcat gccctcgtcc atgttccgcg cgggcgcgct
     7501 cgcagtcgcc aacttctgga aagaagccaa gcgcgagggc aacccccagc tgagcctgcg
     7561 catcccgatc ctgatcgacg gcgtccacca agcagccgtc gacaccgcgc tgctcggcac
     7621 ccgccacgtc ctccgcggcc cactcaccaa catcgtccgc gagctccgcc aataccaaac
     7681 cgcgggctgc gaacacgtgg cgctggaagt cagctacagc acctaccccg ccatcctgga
     7741 aacgatcgac atcatcgccg aggaaatccg ccccgccctg gaacagcagc gcgaacgcta
     7801 gggaatcaca cgacgacacg ggaatgcggc cgcccgccgg ccgggcgggg ggcagggggt
     7861 cggttcaccc gttccccgcc gaggccagcc agaaacctcc cctgcccgga ttgggaactc
     7921 ctcccgcaac aaagcgagaa cgcgtgtcgt ggtgagagac cccccgcctg gccggcgggc
     7981 ggccgccggc tcagacgcgc cgcttctcga gcccctgcca gtcgaaggcg atgccgtggc
     8041 cgggacggtc cggggcgacg gcgcgaccgt cttcgaggcg cagcggctcg gcgatgtagc
     8101 ggtcgagacc gaagccgtgg gcttcgaggt acgagcgatt cgggacggcg ccgagcaggt
     8161 gcaccgtgat gtcgtgggca ccgtgggacg tgaccgggag attgaaggct tccgctagat
     8221 gacagatctt catgaatgac gtgatgccgc cgcaattggt gacgtcgggc tcggggaagg
     8281 tcacgccgcc ggccgcgatg agggcgcgga actcgtagag cgtgtggagg ttctcgcctg
     8341 cggcgatggg caggccccct tcccgcacga tgcgggcgtg gccggggacg tcgtcgggga
     8401 tcgtgggctc ctcgagccag accgggttga ggtcctggag cgcgcgcgcc gcgcggatgg
     8461 cgtcgtccac gctccagcgc atgttggcgt ccaccatcaa cggaaactcg gggccgaggt
     8521 ggccgcgcat ggcgcggacg cgctcgacgt cctcgaggag cgaggagcgg cccaccttca
     8581 tcttgatggc gcggaagccc cgggccaggt tgtcgtccgt ctgacgcagg agggcgtcga
     8641 gcgggaactc gaggtcgatg ccgccggcgt agcacggcac gcgggggtcg aagccgccca
     8701 gcacgcgcca gagcggcgcg ccctgccgcc gcgcacgcag gtcccacagg gcgatgtcca
     8761 ccgccgagat cgccaggccc tgggcgccgc cgcggccgcc gtaatggagc gcccaccaca
     8821 tcgcctgcca cagctcctcg atgcgctcgg cctcccggcc ggtcaggcgg agcgcgaggt
     8881 cgcgggagag caccgcgtgc accgcggacc cgccggtccc caccgtgtag gtgtagccga
     8941 cgccctcggc gccgtcctca tcgcgcaggc gcaccgtgac cagctcgaag ccctcgatca
     9001 cgccgtgggt gctgtcggag agcggcaccg gcagcgggat gcggtagtgg tcggcccgta
     9061 cctcgcggag gcgcatcagg cggtggcgag gacgatcttg ccgaactggc cggcctcttc
     9121 catgtgccgg tgcgcggcgg cggcctcggc gagcgggaag atccgatcca cggccgggcg
     9181 cagcccgtct cgcgcgtaga gctccagcat ggccccgaac tcggccgggc tccccatggt
     9241 ggtgcccagc accgagatct gccgccagaa caccgcgcgc aggacgagct cgcgcaccgg
     9301 acccgtggtg gcgccgtagg tcgcgatgcg cccgcccggg cgcagcacgt ccaccgcgcg
     9361 cgcgaaggtc tccccgccca cgctgtcgat gaccacgtcg ggcccgcggc cgccggtgag
     9421 cgcgaggatc tccttcgccc agtcctgcgt gcggtagttg gctccgcccg ccgcgccgag
     9481 ctcacgggcg cgggcgagct tcgcgtc
//
LOCUS       DASUFA010000183         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_960309, whole genome shotgun sequence.
ACCESSION   DASUFA010000183 DASUFA010000000
VERSION     DASUFA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960309"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFX87_06530"
     CDS             complement(<1..732)
                     /locus_tag="VFX87_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX4994605.1"
                     /translation="MLTGRRFTLPLQALEPMDLEGDGVATLLLLNPTGGLLAGDRLET
                     HIVLGPGSRVCLSTPSATRVYRSPGPVAVQRVTMDIGRSAALEWLPDHLIPSPGARLR
                     QTTEIRLAADATLLYLDAWATGRAARGEAWGFDLLDSSLLVRDEAGLLLRERSILAGH
                     PRRDGLGGTEGFAYAATFVALRPAGEGWDELARALQVEVDVAGVGGRAGVTTLARGGL
                     VARLLCPSAPTLQATVQALWAGCRRR"
     gene            complement(809..1426)
                     /gene="ureG"
                     /locus_tag="VFX87_06535"
     CDS             complement(809..1426)
                     /gene="ureG"
                     /locus_tag="VFX87_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX4994606.1"
                     /translation="MTGLPRPLKIGIGGPVGSGKTALVETLSLRLRDDFDMAVITNDI
                     YTQEDAQFLIRRGALPAERVVGVETGGCPHTAIREDASVNLEAVHGLMGRFPGLELLF
                     VESGGDNLAATFSPELVDATIYVIDVAQGEKIPRKGGPGIMRSDLLVINKIDLAPHVG
                     ADLGVMERDAKRMRGERPFVFTNLREGTGVDAVVSWIRRDLLFEP"
     gene            complement(1423..2112)
                     /locus_tag="VFX87_06540"
     CDS             complement(1423..2112)
                     /locus_tag="VFX87_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEX4994607.1"
                     /translation="MADPRLVSLLHFADSAFPTGGYAHSFGLEHYCQAGIVRDRDGLE
                     RFLLTQLEGAAGPCDATAAAGTLRALMRGDLEACHRLDDALEAMKVVKEFREGSRQMG
                     RQTLRVAAALIGEPRLAAYLDEVNAHRAPGHHAAAFGMVAGALGWEAEAAVTAFLYST
                     TALLVGAALRLLPMGQLEGQAVLWRLHPVIERVAREAAERSPADLWSFAPGLDIQGML
                     HERLDARLFRS"
     gene            complement(2105..2545)
                     /locus_tag="VFX87_06545"
     CDS             complement(2105..2545)
                     /locus_tag="VFX87_06545"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX4994608.1"
                     /translation="MRVITGAPITVDPVTLVFRSRDTLRLTWEERRWTRKKVVTTAGR
                     EIALALPTGTVLEPGDVLAVEENWYLAVEARPEPVLVLFPESYEAAVRIAFDVGNRHF
                     SLASDGDTLLVPDDTAMEDLVRRLGVRWERCEMVYAPIDGSDDG"
BASE COUNT          389 a         1006 c          962 g          357 t
ORIGIN      
        1 tcgtcgccgg cacccagccc acagcgcctg caccgtggcc tggagcgtgg gcgccgacgg
       61 acacagcagg cgggccacca ggccgcctcg ggcgagcgtg gtcacccccg cgcgtccgcc
      121 gacgcctgcg acatctacct ccacctggag cgcgcgggcc agctcatccc acccctcgcc
      181 ggccggccgt aacgccacga atgtcgccgc gtaggcgaag ccctccgtgc cgcccaggcc
      241 gtcccggcgc ggatgcccgg ccaggatcga gcgctcccgg agcaagagcc cggcctcgtc
      301 gcgaaccagc aggctgctgt ccagcaggtc gaagccccac gcctccccgc gcgccgcgcg
      361 tcccgtcgcc caggcgtcga ggtagagcag cgtggcgtcg gcggcgagcc ggatctcggt
      421 cgtctgccgg agacgcgcgc ccggcgacgg gatcaggtga tcgggcagcc actcgagcgc
      481 cgcgctgcgt ccgatgtcca tggtgacccg ctggacggcg acgggccccg ggctccggta
      541 cacccgcgtg gcggagggcg tggacaggca gacgcggctg cccggcccca gcacgatgtg
      601 ggtctcgagc cgatcgccgg ccagcagccc gcccgtcggg ttcaggagca gcagcgtggc
      661 gacgccgtcg ccctcgaggt ccatcggctc gagcgcctgg agcggcagcg tgaagcgccg
      721 gcccgtgagc acggtgcgcc caccccgggc ctcgaaggcc aggtggaggc gcccgtcccg
      781 cccggttcgg ccgatcgcgg gccgcgggtc agggctcgaa gagcaggtcg cgtcggatcc
      841 aggagaccac ggcgtccacg ccggtgccct cccggagatt ggtgaagaca aaagggcgct
      901 ctccccgcat gcgcttggca tcgcgctcca tcaccccgag atcggcaccg acgtggggcg
      961 cgaggtcgat cttgttgatc accaggaggt cggagcgcat gatgcccggt cctcccttgc
     1021 gggggatctt ctccccctgg gccacgtcga tgacgtagat cgtggcgtcc accagctccg
     1081 ggctgaaggt cgcggcgaga ttgtcgccgc cgctctccac gaagagcagc tcgagccccg
     1141 ggaaccgtcc catgaggccg tgcaccgcct ccagattcac cgacgcgtcc tcgcggatgg
     1201 cggtgtgcgg gcagccgccc gtctccacgc ccaccacgcg ctcggcgggc aaggctcccc
     1261 gccggatgag gaactgggcg tcctcctgcg tgtagatgtc gttggtgatc accgccatgt
     1321 cgaagtcgtc ccgcaggcgc agggacaggg tctccaccag cgcggtcttg ccggagccga
     1381 ccggaccgcc gatgccgatc ttgagcggcc ggggcagccc ggtcatgagc ggaacaggcg
     1441 cgcgtcgagg cgctcgtgga gcatgccctg gatgtcgagc ccgggcgcga agctccagag
     1501 atcggcgggc gaccgctcgg cggcttcgcg cgccacgcgc tcgatcacgg ggtggaggcg
     1561 ccacaggacg gcctgcccct cgagctgccc catgggaagg agtcggaggg cggcgcccac
     1621 cagcagcgcg gtggtcgagt agaggaaggc ggtcaccgcg gcctccgcct cccagccgag
     1681 cgcgcctgcg accatgccga aggccgccgc gtggtggccg ggagcgcggt gcgcgttgac
     1741 ctcgtccaga tacgcggcca ggcgcggctc gccgatgagc gccgcggcca cgcgcagggt
     1801 ctggcgcccc atctgccggc tgccctcacg gaactccttc acgaccttca tggcctcgag
     1861 cgcgtcatcc agtcggtggc acgcctcgag atcgccgcgc atcagggccc gcagcgtccc
     1921 cgccgcggcg gtggcatcgc aggggcccgc cgcgccctcg agctgcgtca gcaggaagcg
     1981 ctcgagcccg tcccggtccc gcacgatccc ggcctggcag tagtgctcga gcccgaacga
     2041 atgcgcgtag ccgccggtgg gaaaggcgct gtccgcgaag tggagcagcg agaccaggcg
     2101 ggggtcagcc atcgtcgctc ccgtcgatcg gcgcatagac catctcgcac cgctcccagc
     2161 ggacgccgag ccgcctcacc aggtcctcca tcgcggtgtc gtcgggcacc agcagcgtgt
     2221 ccccgtccga ggccagggag aagtgccggt tccccacgtc gaaggcgatg cgcaccgccg
     2281 cctcgtagct ctcgggaaag aggacgagca cgggctcggg tcgggcctcg acggccaggt
     2341 accagttctc ctccaccgcg agcacgtcgc ccggctcgag cacggtgccg gtcggcaggg
     2401 ccagcgcgat ctcgcggccg gccgtggtca cgaccttctt ccgcgtccag cgccgctcct
     2461 cccaggtcag gcggagggtg tcgcgtgacc tgaacacgag agtcaccggg tcaaccgtga
     2521 tcggcgcccc ggtgatcacc cgcatcgcgc tagaacagga agtagcgctg agccatcggc
     2581 agcaccgtgg ccggctcgca ggtcagcagc tggccatcgg cgcgcacggt gtaggtctgc
     2641 gggtcgattt ccatcgccgg cagcgcggcg ttgtggatca tctgcgcctt cttcaccgtg
     2701 cggcagccct tcac
//
LOCUS       DASUFA010000184         7641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_726204, whole genome shotgun sequence.
ACCESSION   DASUFA010000184 DASUFA010000000
VERSION     DASUFA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726204"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFX87_06550"
     CDS             <1..1089
                     /locus_tag="VFX87_06550"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4994609.1"
                     /translation="PRCMDLALEALDLPAARKEQARLRAQGRYLGIGVVNYVEATATV
                     PNESAVVRVHADGGVTVVSGAAPQGQGHVTMFSRLVGRQLGVEPDAIEVLTGDTALVH
                     TGGGTYGSRTAAIGGSAALLASRAVLEKARRVAAHLLEAAPADVVVEHGMFAVRGLPS
                     RTLGWREVAAAAHAGRVPGEAAGLEDTQVFSVSQSSYANGTHAVVLEVDPETGAVQIL
                     RWIVAHDCGHVLEPGIVDGQIHGAVVQGLPDALNVQLAYDDGGQRLTATLMEYALATA
                     ADAPPVFELRHLETPTPLNPLGAKGAGEGGIMPVHPLIAQALEDALAPFGCARIERVP
                     VTRADIHALVQAAERLDSSRGGRVDVAP"
     gene            1086..1913
                     /locus_tag="VFX87_06555"
     CDS             1086..1913
                     /locus_tag="VFX87_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX4994610.1"
                     /translation="MKALSPEAVARYRRDGFYFPLRVLSLEEAAGYRARLEAVERERA
                     GPLGGELRHKGHLLFTWLDALIRHPRILDAVEDLLGPNLLCWSSSFFIKEAHDPAFVS
                     WHQDSTYWGLSEPDVMTAWVAFSESSIESGAMRMIPGTHTEQVAHRDTFAPDNLLSRG
                     QEIMVDVDEARAVDMVLHPGEMSLHHVRMFHGSPPNRSADRRIGYAIRYIATHVRQVA
                     GPRDSAMLVRGVDAYHHFDPEEPPAADLDPAARARHAAIMKRQGELLYRGTSAERFR"
     gene            complement(1880..2197)
                     /locus_tag="VFX87_06560"
     CDS             complement(1880..2197)
                     /locus_tag="VFX87_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994611.1"
                     /translation="MTDTLPTKRWTRREYDRLIEEGAFGPDDRIELLGGALVVREPQG
                     SSHAMGIRMAEEALRRVFATGWDVRGRYRHAARRARLPGLRRRPDPLDPRRYRNRSAL
                     VPR"
     gene            2288..3160
                     /locus_tag="VFX87_06565"
     CDS             2288..3160
                     /locus_tag="VFX87_06565"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994612.1"
                     /translation="MPVALVTGTSTGIGFATALHLARHGHRVVATMRNLAKAGPLEEA
                     ARRESLPLILRELDVTRQDSIDRAVAATREHEGAVDVLVNNAGIGGATPLELTPEAEH
                     RAMFEANYWGPIRLIQAVLPAMRERRAGCIVNVTSIAGRVATPNQIAYSASKHALAAA
                     SEALAHEVAAFGICVAIIEPGVIQTAIFENSAGATRYDKTSPYRQIMRRNGKLFAAGF
                     RDPGKAETVAAAILHAITTDRPRLRYLVGVDAEGLASGRARISDEEWVAMGGELSDEE
                     YNARFKRYFGIELR"
     gene            complement(3157..4224)
                     /locus_tag="VFX87_06570"
     CDS             complement(3157..4224)
                     /locus_tag="VFX87_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994613.1"
                     /translation="MRMSVCVMADIDEIGFYPHIESLGYDSAWVADSQMLYSDCYSVL
                     ALAARQTSRLRLGPGVAICGTRIPPVHVAAMATLNRLAPGRVHLGIGTGNTAMRSMGH
                     PPMRIADYAEYLRVLRGLLQGDAVDYVYEGTRHPIKMLIREKKYMSLEPRIPLYVSAF
                     GPRAMELAGQYGDGLVFAIPPRGLAPAEALALAQAGAGRGGRTLGNDFHMCALTSIVL
                     LEPGEAADSERVIRAVGPNVMASVYYFYDAVHERGIEPPPFLRRIWRRYCALVEEMPA
                     EHRHFRTHEGHYTYIHPGEAELIDADLVRDTCLVGSAEELIERIQELERGGLRELMWA
                     TGTDEKWRFSQQLADRVMARL"
     gene            complement(4267..5010)
                     /locus_tag="VFX87_06575"
     CDS             complement(4267..5010)
                     /locus_tag="VFX87_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994614.1"
                     /translation="MRPLLLLALLLLGGLAPATAQPATPAETARAMLRQYQDDPARID
                     RTRDLLEAHVARGVVDVPTLLALARAWFLFGEERAKTEDDKVAAYARARDYAKRATEM
                     APNNADAHLWYAITLGTWSQAKGLLRSALALRDLRKEVDIVLRLDPSNIEAHIMAGSI
                     ARELPVLLGGDAREAEERFKTAQRLDPRLTGVRIELAQLYINMGRHAEARQQLQAVLA
                     ERAPTDRPRWTLKEVPRARQMLESIRDKK"
     gene            5212..6822
                     /locus_tag="VFX87_06580"
     CDS             5212..6822
                     /locus_tag="VFX87_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4994615.1"
                     /translation="MARRKEKGPRADLAEGAGRLAAILDSLDDAFVGKTLDGVITSWN
                     RAAERIFGYTATEAVGQHISLIIPEERRGEEVGVLARLVKGERIDHFETVRQAKDGRR
                     VDISLTVSPVRNSAGRIVGASKVARDISDRRRLERERQALLEREQEARAEAEALNRSK
                     DQFLATLSHELRTPLNAIYGWARMLDAGGLDETATRRATEAILRNAAAQVQLIEDLFD
                     VSRVITGNMRLDVRPLNVTGVLEVALDAVRPAAAAKGIHLESHLDPHAGAIVGDPARL
                     QQVAWNLLMNAVKFTPRAGRVDVHLRRAGTHIEIVVSDSGEGIAPEHMPRIFQRFGQA
                     DGSSTRRYAGIGIGLALVRHLVELHGGTVSVHSAGLGLGATFTVFLPVTTEPALTPPA
                     ASDNASAPGSDRVKPISLRDLRILVVDDDADGLELAALVLINAGAEVRAAPSSGAAIA
                     VLEDWPADVLVTDLAMPGEDGVGLLRRARRVMTLRGRRLPALALTAYGRSEDRVRALT
                     VGFNLHLGKPVNPAELVLAVASLGGRTE"
     gene            complement(6844..7239)
                     /gene="rnpB"
                     /locus_tag="VFX87_06585"
     ncRNA           complement(6844..7239)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFX87_06585"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7284..>7641)
                     /locus_tag="VFX87_06590"
     CDS             complement(7284..>7641)
                     /locus_tag="VFX87_06590"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEX4994616.1"
                     /translation="GRKRTTATRRGREGAEEQGRTVGLLREVLEHHETGPAASAGDLD
                     ADWQRAQSSGEEAAGGSVSTPDQDRVDDIGHALGVEQPSTAPLRSSEEMLEERDRRYW
                     ELERRAQDKAVKRGEP"
BASE COUNT         1046 a         2709 c         2781 g         1105 t
ORIGIN      
        1 ccgcgctgca tggacctggc gctcgaggcg ctcgatctgc ccgcggcccg gaaggagcag
       61 gcgcggctcc gcgcgcaggg gcggtacctc ggcatcggcg tcgtcaacta cgtggaggcc
      121 accgccaccg tgccgaacga gagcgcggtg gtgcgcgtcc atgccgacgg cggcgtgacc
      181 gtggtcagcg gcgccgcgcc gcaggggcag gggcacgtca cgatgttctc gcgcctggtc
      241 ggccggcagc tcggcgtcga gcccgacgcc atcgaggtgc tgaccggcga caccgcgctc
      301 gtgcacaccg gcggcggcac ctatggcagc cgcaccgccg cgatcggcgg cagcgcggcg
      361 ctcctggcct cgcgcgcggt gctggagaag gcgcggcgcg tggccgcgca cctgctcgag
      421 gcggcgccgg ccgacgtcgt ggtcgagcac ggcatgttcg cggtgcgcgg gctgccgagc
      481 cgcacgctgg gctggcgcga ggtcgcggcc gcggcgcacg ccgggcgggt gcccggcgag
      541 gccgccggcc tcgaggacac ccaggtcttc agcgtgtcgc agtcgagcta cgccaacggc
      601 acccacgcgg tggtgctcga ggtggatccg gagaccggcg cggtccagat cctgcgctgg
      661 atcgtggcgc acgactgcgg ccacgtgctc gagccgggca tcgtggacgg ccagatccac
      721 ggcgcggtgg tgcagggcct gccggacgcg ctgaacgtcc agctcgccta cgacgacggc
      781 ggccagcgcc tcaccgcgac cctcatggag tacgcgctcg ccaccgcggc cgacgcgccg
      841 cccgtcttcg agctgcgcca cctcgagacg ccgacgccgc tcaacccgct gggcgccaag
      901 ggcgcgggcg agggcggcat catgccggtg catcccctca tcgcccaggc cctcgaggac
      961 gcgctcgccc ccttcggttg cgctcgcatc gagcgcgtgc cggtgacgcg tgcggacatc
     1021 cacgccctgg tgcaggccgc ggagcgactt gacagctcgc gaggcggccg ggtagacgtg
     1081 gcgccatgaa agccctctcc cccgaggcgg tggcgcggta ccgccgggac ggcttctact
     1141 tcccgctccg cgtgctctcg ctcgaggagg cggccggcta tcgcgcccgg ctcgaggcgg
     1201 tggagcgcga gcgggctggc ccgctgggcg gcgagctgcg gcacaagggc catctcctct
     1261 tcacctggct cgacgcgctg atccggcatc cgcgtattct cgacgcggtc gaggatctcc
     1321 tcggtccgaa tctcctctgc tggagctcga gctttttcat caaggaggcg cacgatccgg
     1381 cgttcgtgtc ctggcaccag gactcgacct actgggggct cagcgagccg gacgtgatga
     1441 ccgcgtgggt ggcgttctcg gagagctcga tcgagagcgg cgcgatgcgc atgatcccgg
     1501 gcacgcacac cgagcaggtc gcgcatcgcg atacgttcgc gccggacaac ctgctctcgc
     1561 gtgggcagga gatcatggtc gatgtggacg aggcgcgcgc ggtggacatg gtgctgcacc
     1621 cgggcgagat gtcgctgcac cacgtgcgca tgtttcacgg ctcgccgccc aaccgctccg
     1681 ccgatcgccg catcggctat gcgatccgct acatcgcgac gcacgtccgc caggtcgcgg
     1741 ggccgcggga cagcgccatg ctcgtgcgcg gggtcgacgc ctaccaccac ttcgaccccg
     1801 aggagccgcc cgcggccgac ctcgatcccg cggcgcgggc gcggcacgcc gccatcatga
     1861 agcgccaggg cgagctgctc taccgcggca ccagcgcgga gcggttccgg tagcgccgcg
     1921 ggtctagggg atcaggtcgg cgacggagac ccgggaggcg ggcgcggcga gcggcgtgac
     1981 ggtatcggcc gcgcacatcc caaccggtcg cgaacacccg gcgtagcgct tcttccgcca
     2041 tgcgaatgcc catggcgtgg gagctgcctt gcggctcccg caccaccagc gccccgccga
     2101 gcagctcgat gcggtcgtcc ggcccgaagg cgccctcctc gatgagccgg tcgtattcgc
     2161 ggcgcgtcca gcgcttggtc ggcagcgtgt cggtcatcgc ctcgagggta gtcccggccg
     2221 cccggtcccg cggcccctgc gccttcgccg cttgacgcca cgcgccgccg agggcagcat
     2281 ggcccccatg cccgtcgccc tcgtcaccgg caccagcacc ggcatcggct tcgccaccgc
     2341 gctgcacctc gcgcgtcacg gtcaccgcgt ggtggcgacc atgcgcaacc tggcgaaggc
     2401 cggcccgctc gaggaggcgg cgcgccggga gagcctgccg ctcatcctgc gtgagctgga
     2461 cgtcacccgc caggactcca tcgaccgggc ggtggccgcg acgcgggagc acgagggcgc
     2521 cgtcgacgtg ctcgtcaaca acgcgggcat cggcggggcg actccgctcg agctcacgcc
     2581 cgaggccgag caccgcgcga tgttcgaggc caactactgg ggtccgatcc gcctgatcca
     2641 ggcggtgctg ccggccatgc gcgagcggcg ggccgggtgc atcgtcaacg tcacgtcgat
     2701 cgcggggcgg gtggccaccc cgaaccagat cgcgtactcc gcgtccaagc acgccctggc
     2761 cgcggcgagc gaggcgctgg cccacgaggt ggccgccttc gggatctgcg tggccatcat
     2821 cgagccgggc gtgatccaga ccgcgatctt cgagaactcg gcgggcgcca cccgctacga
     2881 caagacctcg ccgtaccggc agatcatgcg ccgcaacggc aagctcttcg cggccggctt
     2941 ccgcgacccg ggcaaggcgg agaccgtcgc ggcggcgatc ctccacgcca tcaccaccga
     3001 ccggccgcgg ctgcgctacc tggtcggcgt ggacgcggaa ggcctggcct cgggacgcgc
     3061 gcggatcagc gacgaggagt gggtcgcgat gggcggcgag ctgagcgacg aggagtacaa
     3121 cgcccgcttc aagcgctact tcgggatcga gctccgctag aggcgggcca tcacccggtc
     3181 cgcgagctgc tggctgaacc gccacttctc gtcggtgccg gtggcccaca tcagctcccg
     3241 caggccgccg cgctccagct cctggatccg ctcgatcagc tcctccgcgg agcccaccag
     3301 gcaggtgtcg cgcaccaggt cggcgtcgat cagctcggcc tcgccgggat ggatgtaggt
     3361 gtagtggccc tcgtgcgtgc ggaagtgccg gtgctcggcc ggcatctcct ccaccagggc
     3421 gcagtagcgc ctccagatgc gccgcaggaa gggcggcggc tcgatgccgc gctcgtgcac
     3481 cgcgtcgtag aagtagtaca cgctggccat cacgttgggc cccaccgcgc ggatgacgcg
     3541 ctcggagtcg gccgcctcgc ccggctcgag caggacgatg ctggtgagcg cgcacatgtg
     3601 gaagtcgttc ccgagcgtgc ggccgccgcg cccggccccg gcctgcgcca gcgcgagggc
     3661 ctcggcgggc gccagcccgc gcggcgggat cgcgaagacg agcccgtcgc cgtactggcc
     3721 cgccagctcc atggcgcgcg gcccgaaggc cgagacgtag agggggatgc gcggctcgag
     3781 gctcatgtac ttcttctcgc ggatcagcat cttgatcggg tggcgtgtgc cctcgtagac
     3841 gtagtccacc gcgtcgccct ggagcaggcc gcggagcacg cgcaggtact cggcgtagtc
     3901 cgcgatccgc atcggtgggt ggcccatgct gcgcatggcg gtgttgccgg tgccgatgcc
     3961 gaggtgcacg cggcccggcg ccagccggtt gagcgtggcc atggccgcca cgtgcacggg
     4021 cgggatgcgc gtgccgcaga tggccacgcc cgggcccagg cgcagccgcg acgtctggcg
     4081 cgcggccagg gccagcaccg agtagcagtc ggagtacagc atctggctgt cggcgaccca
     4141 tgccgagtcg tagcccaggg actcgatgtg ggggtagaac ccgatctcgt cgatgtcggc
     4201 catcacgcac acgctcatgc gcattgtggt tcctctccgg gtctggggcc ctctccgggt
     4261 ctggggtcac ttcttgtcgc ggatcgattc gagcatctgc cgggcgcgcg gcacttcctt
     4321 gagcgtccag cgcggccggt cggtgggcgc gcgctcggcc aggacggctt ggagctgctg
     4381 ccgcgcctcc gcgtggcggc ccatgttgat gtaaagctgc gccagctcga tgcgcacgcc
     4441 ggtcagcctg gggtcgagcc gctgcgcggt cttgaagcgc tcctccgcct ccctggcgtc
     4501 gccgcccagc agcaccggca gctcgcgggc gatgctcccg gccatgatgt gcgcctcgat
     4561 gttgctgggg tcgagccgca gcacgatgtc gacctccttg cgcagatcgc ggagggccag
     4621 ggccgagcgg agcaggccct tggcctgcga ccacgtgccg agggtgatgg cgtaccagag
     4681 atgggcgtcg gcgttgttcg gggccatctc ggtggcgcgc ttggcatagt cgcgcgcccg
     4741 cgcgtaggcc gccaccttgt cgtcctcggt cttcgcgcgc tcttcgccga agagaaacca
     4801 ggcgcgggcc agggccagga gggtgggcac gtccacgacg ccccgggcta cgtgggcctc
     4861 gagcaggtcg cgcgtgcgat cgatgcgcgc ggggtcgtcc tggtactggc gcagcatcgc
     4921 gcgcgcggtc tcggcaggcg ttgcgggctg cgcggtcgcg ggggcgaggc cgcccaggag
     4981 caggagggcg agcaggagga gcggccgcat ggcccgatcg taccggccgg cggcaggggg
     5041 tgggagggaa aatggcgacc cgcgtgcccc tggcaggaga tttgcgagga gtcttctccg
     5101 gccgggtcga accgtccagg tgcgcgaaat ggctgcgacg cacgacgaag ggcggggcga
     5161 cccggtaaaa acgtcaggaa agaaataccg cgggcgggga gaccggccgg attggcaagg
     5221 cgaaaagaaa agggtccgag ggcagacctg gccgagggcg ccgggcgcct ggcggcgatc
     5281 ctcgattcct tggacgatgc cttcgtgggc aagacgctcg acggggtcat cacatcctgg
     5341 aaccgggcgg ccgaacgcat cttcggctat accgcgacgg aagcggtggg ccagcacatt
     5401 tcgttgatca tcccggagga gcggcgcgga gaggaggtcg gggttctggc ccgcctcgtc
     5461 aagggagagc ggatcgatca cttcgagacg gtacggcagg ccaaggacgg ccggcgcgtc
     5521 gatatctccc tcacggtgtc gccggtccgg aactcggccg ggcgcatcgt cggggcctcg
     5581 aaggtggcgc gcgacatatc ggaccggcga cgcctcgagc gcgagcgcca ggcgctcctc
     5641 gagcgcgagc aggaggcccg cgccgaggcc gaagccttga accgaagcaa ggatcagttc
     5701 ctcgcgacgc tgtcgcacga gctccggacc cccctcaacg ccatctacgg ctgggcccgc
     5761 atgcttgacg cgggcgggct cgacgagacg gcgactcgcc gggcgaccga ggcgatcctc
     5821 cgcaacgcgg cggcccaggt gcagctgatc gaggacctgt tcgatgtctc gcgcgtgatc
     5881 acgggcaaca tgcgcctgga cgtgcggcca ctgaacgtca cgggggtgct ggaggtcgcc
     5941 ctcgacgcgg tccgaccggc ggccgcggcg aaggggatcc atctcgagtc gcacctcgat
     6001 ccccacgcgg gcgcgatagt gggagatccc gcccgcctcc agcaggtggc ctggaatctg
     6061 ctcatgaacg cggtcaagtt cacgccgagg gcgggccgag tcgacgtgca cctgcggcga
     6121 gccggcaccc acatcgagat cgtggtgagc gacagcggag agggcatcgc gcccgagcac
     6181 atgccgcgca tcttccagcg gttcggccag gccgacggca gctcgacacg ccggtacgcc
     6241 gggatcggca tcgggctcgc gctcgttcgg catctcgtcg agcttcacgg aggcacggtg
     6301 agtgtccaca gcgcgggcct cgggctcggg gccacgttca cggtgttcct gccggtgacc
     6361 acggagcctg cgctcacgcc ccccgccgcg agcgacaatg ccagcgcgcc cggatccgat
     6421 cgagtcaagc cgatctcgct ccgcgatctc cggatcttgg tcgtggatga cgatgcggac
     6481 ggcctggagc tggccgcgct cgtgctcatc aatgcggggg ccgaggtgcg ggcggcgccg
     6541 tcctcggggg ccgcgatcgc cgtgctcgaa gactggcccg ccgacgtgct ggtgacggac
     6601 ctcgcaatgc cgggcgagga cggcgtgggt ctcctgcggc gggcccgccg ggtgatgacg
     6661 ctgcgcgggc gccggcttcc ggcgttggcc ctgaccgcgt acgggcgatc ggaggaccgg
     6721 gtccgggcgc tgacggtcgg cttcaacctg cacctgggca aaccggtcaa cccggcggag
     6781 ctggtgctgg cggtggcgag cctaggcggc cgcacggagt agcgggccgg gatctcttaa
     6841 atgcgccaga gagggcctgt gagccgggtt ctgtctgacc gccccggttt cccgtgacgg
     6901 cgagatgatc atttctctag gacgccggtt acccggcgcc tcaagcggcc atacccgaga
     6961 gcaccacgcg ggccgcgctt cacctgctct tgcgagcagg atcgctctcc tatttggcct
     7021 tgctccgggt ggggtttacc gagccgacct ggtcacccag gccgctggtg agctcttacc
     7081 tcaccgtttc acccttaccg cccgtgaggg cggcggtttg ctttctgtgg cactgtccat
     7141 ggggtcaccc cccctggccg ttagccagca ccctgccctg cggagcccgg actttcctcc
     7201 cgcacggtcg cccgtgccgg cgatcatcca gccttctctg gcgcaatcaa agagcgtgtg
     7261 tcgctgcggg aaagtatagc ccgtcacggc tcgccccgct tgaccgcctt gtcctgcgcc
     7321 cggcgctcga gctcccagta ccgccggtcg cgctcttcca gcatctcctc cgaggagcgg
     7381 agcggcgccg tcgatggctg ctccacgccg agcgcgtggc cgatgtcatc cacccggtcc
     7441 tggtcggggg tggacacgct gccgcctgcc gcttcctcgc ccgagctctg cgcccgctgc
     7501 cagtccgcgt ccaggtcacc ggcgctcgcc gccggcccgg tctcatggtg ctcaagcacc
     7561 tcgcgcagca ggccgaccgt tcggccctgc tcctccgcgc cctcccggcc gcgtcgggtg
     7621 gccgtcgtgc gcttgcgccc g
//
LOCUS       DASUFA010000185        18094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1630038, whole genome shotgun sequence.
ACCESSION   DASUFA010000185 DASUFA010000000
VERSION     DASUFA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 18094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18094
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630038"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFX87_06595"
     CDS             <1..228
                     /locus_tag="VFX87_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4994617.1"
                     /translation="AAIVARARKAGVRATGLLLEGVPHERVARAARARKADLLVIGTH
                     GRTGLAKFFLGSVATRLVASAPCPVLTVRGR"
     gene            225..614
                     /locus_tag="VFX87_06600"
     CDS             225..614
                     /locus_tag="VFX87_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994618.1"
                     /translation="MTTVAAASITADSKINDVLAKHPGTAPVFSQGRRLYVDQPRELY
                     ARFPGLSVGDFARQNGMDPGPLLAQLNALAESEAGARASGAHGTGESRAGQFSLTLGY
                     TCSHRPRDDAAPDSVSVVAVQSAHGPE"
     gene            821..1405
                     /locus_tag="VFX87_06605"
     CDS             821..1405
                     /locus_tag="VFX87_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX4994619.1"
                     /translation="MFGSARFKTDHPYYELGREVGARLARRGFTVMTGGGPGIMEAAN
                     RGAKDVGGRSIGCNIELPQEQQPNPYVDRFVTFRHFYVRKVMLVKYSYAFIALPGGFG
                     TLDEIFEIATLVQTGKVSEFPIVLMGREYWRPLIDFLRNRLLVEGTIDPGDEQRLQVT
                     DSPEEAVDLVAGVAMRRFGLSYGPQARRRWYLGE"
     gene            1495..2703
                     /locus_tag="VFX87_06610"
     CDS             1495..2703
                     /locus_tag="VFX87_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX4994620.1"
                     /translation="MNTWLTRLAIAFVLTVVAAGPPSAALAQAPIKIGLVQGFTGPFE
                     VYAKQAKRGFELGLDYATGGKKELLGRKIEVLEEDDQLKPDVAKQKVTKLYADDKVDL
                     VVGTTSSAAALAVLPVAAEFKKVLIVEPAVADSITGEHWNRYVFRTGRNSGQDAIANA
                     LAVAKPGVAIATIAQDYAFGRDGVAAYKAAVEKAGAQVVHEEYTPTTATDFTAPIQKI
                     IGALKDKPGPTYVFVIWAGKGGPFGQLVDNRLDKYGITLTSGSNVIDVLKAMKAGKLE
                     GMVGGAYYYYEIPKNPVNDWLVREHMKRFNEPPDFFTCGGFAAAMAVVAGIQKAGGTD
                     TEKLIAAMEGLEFQTPKGKMIFRKEDHQALQSMYSFKMVSKPDVAWLVPTLVRELSLQ
                     ETAPPIMNKR"
     gene            2717..3571
                     /locus_tag="VFX87_06615"
     CDS             2717..3571
                     /locus_tag="VFX87_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994621.1"
                     /translation="MTGVTHVALKNGVSLAVAEWPGPAKGPAVVCVHGLTANHTGWAS
                     LADVLSPVHRLIAYDLRGRGESDKPERGYSLLHHNEDLLRLLDHFGLRKAVLVGHSLG
                     AHIAVRFAATHPERVARLVLVDGGIDVRYEVIESLRPAINRLGVEFPSLDMFLGFVRM
                     LPMFEGRWNDYLERYFRYDVEELPAGTVRSKAARHAIEEELGNLERERLWVYHHQIKA
                     PTLIFRAPDGLLTPTDCLMTQDEAEAMAHAIPRAKLVVVPGTNHYTVLLGQHPRVKNA
                     LRAFLKGT"
     gene            3637..4359
                     /locus_tag="VFX87_06620"
     CDS             3637..4359
                     /locus_tag="VFX87_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994622.1"
                     /translation="MDHVTLDVAPFTLKSIIGPNGAGKTTFFNLLSGQYRPSEGRILF
                     KGRDITSRGAAARTRLGIGRSFQLTNIFPTLTVLENVRLAVQAQRGLGFTAWRDYRSF
                     PDLEDAAYRLLETVLLPTKWAAPAGALAHGEKRKLELGILLALEPEVLLLDEPTAGMS
                     LEEVPAIIGLIDAIRARRDRTILLVEHKIDMVMALSDSIAVLKDGRLIADGPPAEVSA
                     SAEVQAAYFGSTTPHPRGAPDA"
     gene            4352..5056
                     /locus_tag="VFX87_06625"
     CDS             4352..5056
                     /locus_tag="VFX87_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994623.1"
                     /translation="MPDLLELEDVHTDIGQHHILQGVSLRVRPDAVTVLLGRNGAGKT
                     TTMRTIMGVLRPRRGAVRFDGRPIHALAPHAIARLGIGYVPEGQGIFATLTVDENLRV
                     AMLAETAESRDRLRRALALFPDLERYRDARAGTLSGGQKQMLAIARAFVNPTRLLLID
                     EPSKGLAPIVIEHLIGALAAMKEHATVVLVEQNFAMASRLGDDCYVMDDGRTVHAGTM
                     AALAADQALKRRYLGV"
     gene            5053..6027
                     /locus_tag="VFX87_06630"
     CDS             5053..6027
                     /locus_tag="VFX87_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994624.1"
                     /translation="MRRALPPLLGLALLLLPLAAIEPRTWLTLTVAGLAMGMLIFLVA
                     AGLTLIFGLMDVLNFAHGALFSWGAYAGFSVAILVNGSWGWAAAESVLANAGLLVLAI
                     GGAVVVSCGLGIALERVIVRPVYGQHLFQILITLGATIVLEELIRIAWGPNDQVMPVP
                     VTFKGSWDVADVVILRFPIIAIALGVSVYAGMWWLLHRTRVGLIVRAGVENNEMVQVM
                     GHDIHRYFTAVFAAGSALAGVGGLMWGMFSQSIRPAMGGEQLIFAFIVVIIGGLGSVT
                     GSLVGALLVGLSYNYVAYLVPKAAIGVTMAIMVLVLLVRPTGLYGTGK"
     gene            6024..7124
                     /locus_tag="VFX87_06635"
     CDS             6024..7124
                     /locus_tag="VFX87_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4994625.1"
                     /translation="MSAPTGFTPVRAARAAARRGRWREPTALLRLGVLLAWVLLPTTA
                     PSFQTLDLALKIALFATLAASFDVVIGYTGIVSFGHAMFFGFGAYAVALAVGKLGAPT
                     YGHVLLGFLAGAAVSATVAVVIGAFSLRVKALFFAMITLAFAEFALILAVQWSALTGG
                     EDGLSPKLPGVFAAGASGPFGLSGRLVTYYTVLAVCVGCFLMMRRFVHSPLGRTLQAI
                     RDNELRAEALGYRTFVFQLMASSFGSVVATVLGGCYTLWVRYVNPESALGIPIMLDVL
                     LMVIIGGIGTLYGGIVGAAVLLTARTLLPNLRGLGATVFAGSEILQRLSERWLLYFGI
                     LFILVVFFFPRGVLGTLREVAARRRRGRVGEA"
     gene            7167..8213
                     /locus_tag="VFX87_06640"
     CDS             7167..8213
                     /locus_tag="VFX87_06640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4994626.1"
                     /translation="MPARDYDAIVVGASFGGLAVARQLRGEILLLDRNEVGSVQTSAC
                     GTPLWVPEALGVSASVLQVHERLEIRTPTRTVRYDLSAVPFCTFDYRAFCEGLLAQCR
                     VRFLRASVTGIEDGTVVTGAGRFAAPVIVDCSGWRRALVGGADAEPARHSFGLETHTT
                     LQDEGLTFLLDRQLIPQGLGWIFPVGHGSLIGLGSYAGRSKLKPALDRLLRDHGTAAG
                     SYHGTYFPNRPLRSTVGRVFAVGDAAGQCLPLTAEGIRPALYFGGECGRIVQRVLDGA
                     VSLEAGLEAYQRLVARYRRPYRVLQLAQWMAAHTPTRWFAMLTQLAAMQPFLPRWWPR
                     YGRFGRPSGVPAAT"
     gene            8322..9065
                     /locus_tag="VFX87_06645"
     CDS             8322..9065
                     /locus_tag="VFX87_06645"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX4994627.1"
                     /translation="MSRRTRWGIIIGVAGLFIAGCAWFVMTNAPLYQLLVRLYVDKIY
                     LKKMLREWGVLAPVIFIALQALQVIVSPIPGEATGILGGYLFGQWLGLVYSTIGLTLG
                     SVGAFAIGRWLGAHYVKNLVSPETWNRLGFIVEAEGAVLCFIVYLIPGLPKDIVCYLF
                     GISPMPLWVFALVSGFGRIPGTWVLSAQGAHTAAGDYLQAILVGAVAVAVALPLYYYR
                     HRIMVWFHGRSVARGEGANAKLDREKPDS"
     gene            9415..10431
                     /gene="aroF"
                     /locus_tag="VFX87_06650"
     CDS             9415..10431
                     /gene="aroF"
                     /locus_tag="VFX87_06650"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX4994628.1"
                     /translation="MIIVLKSGISDAEVNDVCRRITEMGYGPHVIRGEFKTIVAAVGE
                     ERGRPDLRQLEALETVESVMPVQQPFKLASREVRRESSEVRINGITVGGRDVVVMAGP
                     CSVESEAQVLEVAEAVKGSGAKILRGGAYKPRTSPYAFQGLKEQGLRYLAEARKRTGL
                     PVVTEVLETESVEVVAEYSDILQIGARNIQNFTLLRRVGEIGKPVLLKRGMATTIQEF
                     LLSAEYILSAGNPNVILCERGIRTFETSTRFTLDLNAVPVLKKLCHLPVVVDPSHGTG
                     HWDLVAPMAKASVACGADGLIIEVHPRPEEALSDGPQSLKPSKFAQLMCELRPVAEAV
                     GRGL"
     gene            10788..11702
                     /locus_tag="VFX87_06655"
     CDS             10788..11702
                     /locus_tag="VFX87_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4994629.1"
                     /translation="MKWTIRLPKDPAQRTLALAIVTSVLFHLFLVVPFIVIPGFLQPA
                     QFSKRGEPLLVDITPDRPEEKAPLGNPSRPVTPPEVRERPPSPPARPPAPARAVEAPR
                     APAPRATPPAPPQAVAKAEPTPEPMVKTPEPRPTAPDPQPPQTAPSQPAVSAPPAAPQ
                     VAVARPLDSMRRQPGGGGGLTGGGRGGVEGEPIPLDTPEPKYQDYFNKIRERIKANWV
                     YPRQAGERGIEGELLIEFHIAKDGRVAYIELRHSSGTAILDDAALTAVKLAQPFPPVP
                     DEIAKQTLAINGQFKYQIVSGLVNQFLR"
     gene            11815..13170
                     /locus_tag="VFX87_06660"
     CDS             11815..13170
                     /locus_tag="VFX87_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4994630.1"
                     /translation="MLVNDVYVLEPVAGRGGLARVATLVRELRRQSPHTLFALAGDTL
                     SPSLLSTLRRGAQMIEGWNAVGLDLATFGNHEFDFGAAVLSERMRESRFPWLSANVLD
                     RATGQPFGGARRGRAWDFDGVRVGVIGLTTPEAARTSNPGPGLRFDDPLAAGRAAFAA
                     LGPLDLRVAVTHLPLRQDRELAAALAVDAILGGHDHDAMLHVEGRAVIVKAGSDALNV
                     GQVEYEVRCGAVIARRQRLIPVDEHLAEAADIVALVRQQAALLARELDVAVGRTAVAL
                     DARESLTRREETPLGQFVAALLRDRVGAQVGLLNSGAIRGNRVIPAGPLTKKDVRELL
                     PFSNTVTLLEVTGAALRAALERSVDELPRPTGHFLQTAGIQMTVDPARPPGQRVGAIV
                     IEGRPLDPAGLYRVAVPDYLARGQDGYPMLAASRVLLAPEDGPGLIETVLDGLAAGRS
                     P"
     gene            13216..13290
                     /locus_tag="VFX87_06665"
     tRNA            13216..13290
                     /locus_tag="VFX87_06665"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13248..13250,aa:Val,seq:gac)
     gene            13417..15216
                     /gene="thrS"
                     /locus_tag="VFX87_06670"
     CDS             13417..15216
                     /gene="thrS"
                     /locus_tag="VFX87_06670"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX4994631.1"
                     /translation="MAESSEEARHLEFAGEFDCLPEPLPTLRHSTSHVMAQAVKRLFP
                     EVKVAIGPAIEDGFYYDFLKATPFTPEDLERIEQVMREIVKADYPFTREELPRDRAIA
                     FFEERGEPFKVEILRGIDAPTVSIYRQGDFLDLCRGPHVTSTGQLKAFKLLSSSGAYW
                     RGDEKNPMLQRIYGTAWLTQEELDKHVWRLEEAKKRDHRKLGRELDLFDFFDIAPGAP
                     FWLPGGMTLVRELEKFARESLDARGYQEIATPLLVNKKLWEQSGHWALYQDNMFKFEV
                     EDEVFSLKPMNCPESALVYKRALRSYRDLPIRYSEMGRDHRNERSGALSGLVRVRQFT
                     QDDAHLYCRPEQVQSEITDLLQLVREWYATFNLQPSYRLATRPPDKLGTERQWDQAED
                     ALHQALRANNLVSDVDKGGGAFYGPKIDIDVEDALGRQWQLATIQVDLTMLPERMQCE
                     YIDTDGQAKRPVVIHRAIFGSYERFVAILTEHFAGAFPTWLAPVQARVLPISEKHAEY
                     GRKVFGALRAARIRAELDDRNEKLGYRIRDAQMRKVPYMLVVGEREAQSGAASLRRRT
                     GEDVGAVPVERIVAELTAEIGSRSATLTVGHAG"
     gene            15260..15760
                     /gene="infC"
                     /locus_tag="VFX87_06675"
     CDS             15260..15760
                     /gene="infC"
                     /locus_tag="VFX87_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX4994632.1"
                     /translation="MRINEGIRVREVRVVSADGEQLGILPIAQALELARQREMDLVEV
                     AAEAQPPVCRIMDFGKYKYMQARRQKDARKKQTIIQVKEVKLGPKTDTHDFDFKAKHV
                     RRFLEEGNKAKVTVRFKGREMAHTELGWKMLNKMVEGMQDIAVIENHPRMEGRMLSMI
                     LSPKPH"
     gene            15830..16027
                     /gene="rpmI"
                     /locus_tag="VFX87_06680"
     CDS             15830..16027
                     /gene="rpmI"
                     /locus_tag="VFX87_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX4994633.1"
                     /translation="MPKMKTKRAAAKRLKATGTGRLKRNRGWKRHKLEAKPPKRKRRL
                     GKAVLVAKVDEPRLKRLVPYL"
     gene            16040..16396
                     /gene="rplT"
                     /locus_tag="VFX87_06685"
     CDS             16040..16396
                     /gene="rplT"
                     /locus_tag="VFX87_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX4994634.1"
                     /translation="MPRAKGGAKTRQRRKKVLKQAKGYFGGRRKLFRPASETVLRAGA
                     FAYRGRKQKKRRARALWIIRVNAACRQVGLSYSVFMAGLKKAGITLDRKILAELAVSD
                     PTAFAKLAETARAQRA"
     gene            16399..17442
                     /gene="pheS"
                     /locus_tag="VFX87_06690"
     CDS             16399..17442
                     /gene="pheS"
                     /locus_tag="VFX87_06690"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX4994635.1"
                     /translation="MAGDPRVQEVLDRALAEVAGARSTSDLEQVRVRVLGRSGQLTTL
                     LRSLGSIPAAERPKIGEQTNQAKASVEAAIARRLEGLKAEERRLTREADRPDLTLPGR
                     RPSPGPVHPITRVTEEIIEVFEGLGFSVAEGPEVESDYYNFAALNFPDDHPARDMQDT
                     FHIGPDTLLRTHTSPVQIRTMKAQRPPVRIICLGKVYRRDILDATHSPMFHQVEGLAV
                     DRHITMADLKATLQLFAREMFGPKSAVRFRPSFFPFTEPSAEVDVRCFKCGGDGCRFC
                     KDSGWLEILGSGMVHPNVLRNVGYDPEEVTGWAFGMGIDRIALLKYEIDDIRLFFDND
                     LRFLQQFAGLPGR"
     gene            17439..>18094
                     /locus_tag="VFX87_06695"
     CDS             17439..>18094
                     /locus_tag="VFX87_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX4994636.1"
                     /translation="MKISFRWLKEFVETELDARAVADRLTNAGIGVEQIAPVVDGLAG
                     VVVGEIEAIERDLGVSAAGHHNQLVRVALPDRRYSVVCGAPNAAAGARAAFAPPGARL
                     PGLGEVKAVKIRGVVSEGILCSEKELGISEDHSGLLLLPAEAPLGADLVRYLGLDDWI
                     LDVEITPNRPDALSVVGVAREVAALTGAPFRFPKVLVTEGEQEASALAVLDIQAPDLC
                     P"
BASE COUNT         2661 a         6333 c         6357 g         2743 t
ORIGIN      
        1 gccgcgatcg tggcccgggc gcggaaggcg ggcgtgcgcg ccaccgggct cctgctcgag
       61 ggcgtgcccc acgagcgcgt cgcgcgggcg gcccgggcca ggaaggcgga cctgctcgtg
      121 atcggcaccc acgggcgcac cgggctcgcc aagttcttcc tgggcagcgt ggcgacgcgg
      181 ctggttgcca gcgcgccgtg tccggtgctg accgtgcggg gccggtgacc acggtggccg
      241 ccgcctcgat caccgccgac tcgaagatca acgacgtgct ggcgaagcat cccggcaccg
      301 caccggtgtt cagccagggt cggcgtctct acgtggatca gccgcgcgag ctctacgcgc
      361 gcttcccggg gttgagcgtc ggcgacttcg cgcgccagaa cggcatggat cccggtccgc
      421 tcctggccca gctcaacgcg cttgccgaat ccgaggcggg ggcccgcgcg tccggggccc
      481 acgggaccgg cgagtcccgc gccgggcagt tctcgctgac gctcggctac acgtgcagcc
      541 atcggccgcg ggacgatgcg gctcccgaca gcgtctcggt cgtcgccgtg cagtcggccc
      601 acggccccga gtagcaagtc tgagcgcgga gcagccggtc tcgggagccc cgccggcctc
      661 ggaccgatcc cagtggggca agggggcggc ggccccggaa gagatccgct tcctgcatgg
      721 tccccagccc cgcaccaccg agctggcccg gaccgtccgg atcttcttcg agttcctgcg
      781 gggattccgc gcgctacact tcgtcgggcc gtgcgtcacc gtgttcggct ccgcccgctt
      841 caagaccgac catccctact acgagctggg ccgcgaggtg ggcgcccggc tcgcgcgccg
      901 gggattcacg gtcatgacgg gcggcgggcc cggcatcatg gaggcggcca accgcggggc
      961 gaaggacgtg ggcgggcgct cgatcggctg caacatcgag ctgccacagg agcagcagcc
     1021 gaatccctac gtggaccgct tcgtcacctt ccgccacttc tacgtccgca aggtcatgct
     1081 cgtgaagtac tcctacgcct tcatcgcgct gcccggcggg ttcgggacgc tcgacgagat
     1141 attcgagatc gccacgctcg tgcagaccgg caaggtcagc gagttcccca tcgtgctcat
     1201 ggggcgcgag tactggcgtc cgctcatcga cttcctgcgc aaccgcctgc tggtggaggg
     1261 caccatcgat ccgggcgacg agcagcggct gcaggtgacg gattccccgg aggaagcggt
     1321 ggacctggtg gcgggcgtgg cgatgcggcg cttcggcctc agctacgggc cccaggcccg
     1381 tcggcgctgg tacctcgggg agtagccccg aatggcttga cccccatgcc cccccttgct
     1441 agcgtaacca cgccgcatgc ccaccacacg gccgacctgg aacggagacc attcatgaat
     1501 acatggctca cccgcctcgc catcgccttc gtcctgaccg tcgtggccgc cggcccgccg
     1561 tcggccgcgc tcgcccaggc cccgatcaag atcggcctgg tgcagggctt caccggtccg
     1621 ttcgaggtct acgccaagca ggccaagcgc ggcttcgagc tgggcctcga ctacgccacc
     1681 gggggcaaga aggagctgct cggccgcaag atcgaggtcc tcgaggagga cgaccagctc
     1741 aagccggacg tggccaagca gaaggtcacc aagctctacg ccgacgacaa ggtggacctg
     1801 gtggtgggca ccacgtcgag cgcggccgcc ctcgcggtcc tgccggtggc cgccgagttc
     1861 aagaaggtcc tgatcgtgga gcccgcggtc gcggacagca tcaccggcga gcactggaac
     1921 cgctacgtct tccgcaccgg ccgcaactcg ggccaggacg ccatcgccaa cgcgctggcg
     1981 gtggccaagc cgggcgtggc catcgccacc atcgcccagg actacgcgtt cggccgcgac
     2041 ggggtcgccg cctacaaggc cgcggtcgag aaggcggggg cccaggtcgt gcacgaggag
     2101 tacacgccga ccaccgccac cgacttcacc gcgccgatcc agaagatcat cggcgcgctc
     2161 aaggacaagc cgggccccac gtacgtgttt gtgatctggg cgggcaaagg gggccccttc
     2221 ggacagctcg tggacaaccg gctcgacaag tacggcatca cgctcaccag cggcagcaac
     2281 gtgatcgacg tcctgaaggc gatgaaggcg ggcaagctcg agggcatggt gggcggggcc
     2341 tactactact acgagatccc caagaacccg gtgaacgact ggctggtgcg cgagcacatg
     2401 aagcgcttca acgagccgcc cgacttcttc acctgcggcg gcttcgccgc cgcgatggcg
     2461 gtggtggcgg ggatccagaa ggcgggggga accgacaccg agaagcttat cgccgcgatg
     2521 gaaggcctcg agttccagac gcccaagggg aagatgatct tccggaaaga ggatcaccag
     2581 gcgctccaga gcatgtactc gttcaagatg gtgtcgaagc cggacgtggc atggctcgtg
     2641 cccaccctcg tgcgcgagct cagcctgcag gagaccgcgc ccccgatcat gaacaagcga
     2701 tgaccgggag caagcgatga cgggcgtcac ccacgtggcg ctcaagaacg gcgtctcgct
     2761 cgcggtcgcg gagtggccgg ggcccgccaa gggcccggcc gtcgtctgcg tccacgggct
     2821 gaccgccaac cacaccggct gggcgagctt ggccgacgtg ctctcgcccg tccaccgcct
     2881 gatcgcctac gacctccggg ggcggggaga gagcgacaag cccgagcgtg gctacagcct
     2941 cctgcaccac aacgaggacc tgctcaggct cctcgatcac ttcggcctca ggaaggcggt
     3001 gctggtcggc cactccctgg gcgcccacat cgcggtccgc ttcgcggcca cgcatccgga
     3061 gcgggtggcc cgcctcgtgc tggtggacgg cggcatcgac gtgcggtacg aggtgatcga
     3121 gtcgctgcgg cccgccatca accggctcgg ggtcgagttc ccgtcgctcg acatgttcct
     3181 ggggttcgtg cgcatgctgc cgatgttcga ggggcgctgg aacgactacc tcgagcgcta
     3241 cttccgctac gacgtcgaag agctgcccgc gggcaccgtc cgctccaagg cggcgcgcca
     3301 cgccatcgag gaggagctcg gcaacctcga gcgggagcgg ctctgggtct accaccacca
     3361 gatcaaggcg cccaccctga tcttccgcgc gcccgacggg ctgctcaccc cgaccgactg
     3421 cctgatgact caggacgagg cggaggcgat ggcccacgcc atcccacgcg ccaagctggt
     3481 ggtggtgccc gggaccaacc actacaccgt gctgctcggg cagcacccca gggtgaagaa
     3541 cgccctccgg gccttcctca aggggacata gcgacggcgt cggacccggt cgtctccacc
     3601 cgcgacctca ccatccgctt cggaggccac gtcgcggtgg accacgtcac cctcgacgtg
     3661 gcgccgttca cgctcaagtc catcatcggt cccaacggtg cgggcaagac caccttcttc
     3721 aacctgctga gcgggcagta ccggccgagc gaaggccgga tcctgttcaa gggccgcgac
     3781 atcacgagcc ggggggcggc cgcccggacg cggctcggca tcgggcggtc cttccagctc
     3841 accaacatct tccccacgct caccgtgctc gagaacgtgc ggctcgcggt gcaggcgcag
     3901 cgcgggctcg ggttcaccgc ctggcgcgac taccggagct tcccggacct ggaggacgcc
     3961 gcgtaccggc tgctcgagac ggtgctgctc ccgacgaagt gggcggcccc ggccggcgcg
     4021 ctcgcccacg gcgagaagcg gaagctcgag ctgggcatcc tgctcgcgct cgagcccgag
     4081 gtgctgctgc tggacgagcc gaccgcgggg atgtcgctcg aggaggtgcc ggccatcatc
     4141 gggctcatcg acgccatccg cgcccgccgg gaccggacca tcctgctcgt ggagcacaag
     4201 atcgacatgg tgatggcgct gtccgattcg atcgcggtgc tgaaggacgg gcggctcatc
     4261 gccgacggcc cgcccgccga ggtgagcgcg agcgcggagg tgcaggccgc ctacttcggg
     4321 agcacgaccc ctcaccctcg gggagcgccc gatgcctgac ctgctcgagc tagaggacgt
     4381 gcacaccgac atcgggcagc atcacatcct gcagggcgtc tccctgcggg tgcgccccga
     4441 cgcggtcacc gtgctcctcg ggcgcaacgg ggcgggcaag accaccacga tgcggacgat
     4501 catgggtgtg ctgcggcccc ggcggggggc ggtgcgcttc gacggccgcc cgatccacgc
     4561 gctcgccccg cacgccatcg cgcggctcgg catcggctac gtgccggagg ggcagggcat
     4621 cttcgccacg ctcaccgtgg acgagaacct gcgggtggcc atgctggccg agaccgcgga
     4681 gagccgcgac cgcctgcggc gcgcgctcgc gctctttccc gacctcgagc gctaccgcga
     4741 cgcccgcgcg ggcacgctct cgggcggcca gaagcagatg ctggcgatcg cgcgcgcctt
     4801 cgtgaatccc acgcgcctcc tgctgatcga cgagccctcg aagggcctgg ctcccatcgt
     4861 gatcgagcac ctcatcggcg cactcgccgc gatgaaggag cacgccaccg tggtcctggt
     4921 ggagcagaac ttcgcgatgg cgagccgtct cggcgacgac tgctacgtga tggacgacgg
     4981 ccgcaccgtg cacgcgggga cgatggcggc gctcgccgcc gaccaggcgc tcaagcggcg
     5041 gtatctcggc gtatgaggcg cgcgctcccg ccgctgctcg gcctcgccct gctcctcctc
     5101 ccgctcgccg cgatcgagcc ccgcacctgg ctcaccctca cggtggccgg cctcgcgatg
     5161 gggatgctga tcttcctggt cgcggcgggc ctcaccctga tcttcgggct catggacgtg
     5221 ctcaacttcg cgcacggcgc gcttttctcc tggggcgcct acgcgggctt cagcgtggcg
     5281 atcctggtca atggatcctg gggctgggcc gcggccgagt ccgtcctcgc caacgcgggc
     5341 ctgctcgtcc tcgcgatagg cggcgcggtg gtcgtgtcgt gtgggctcgg catcgcgctc
     5401 gagcgggtga tcgtgcggcc ggtctacggg cagcacctct tccagatcct gatcacgctc
     5461 ggggccacca tcgtgctcga ggagctgatc cgcatcgcct ggggcccgaa cgaccaggtc
     5521 atgccggtgc cggtcacctt caagggatcc tgggacgtgg ccgacgtggt gatcctccgc
     5581 ttcccgatca tcgccatcgc gctgggtgtc tccgtctacg cgggcatgtg gtggctgctg
     5641 caccgcacgc gggtcggcct gatcgtgcgc gccggggtcg agaacaacga gatggtgcag
     5701 gtgatgggcc acgacatcca ccgctatttc accgcggtct tcgcggccgg ctccgcgctg
     5761 gccggcgtgg gtgggctcat gtggggcatg ttcagccagt ccatccgccc cgcgatgggc
     5821 ggcgagcagc tcatcttcgc cttcatcgtg gtgatcatcg gcgggctcgg gagcgttacc
     5881 ggatccctgg tcggcgcgct gctggtcggc ctgtcctaca actacgtggc gtatctcgtc
     5941 cccaaggcgg ccatcggcgt gaccatggcc atcatggtgc tggtgctgct cgtgcgcccc
     6001 accgggctct atgggacggg caagtgagcg cgccgaccgg attcaccccg gtacgggccg
     6061 cgcgggcggc ggcgcgccgc gggcgctggc gggagccgac cgcgctcctg cgcctgggtg
     6121 tcctgctcgc gtgggtgctc ttgcccacga ccgcgccctc gttccagacc ctcgacctcg
     6181 cgctcaagat cgcgctcttc gccacgctgg ccgcgagctt cgacgtggtg atcggctaca
     6241 ccggcatcgt gtccttcggc cacgcgatgt tcttcggctt cggcgcctac gcggtggcgc
     6301 tggcggtggg caagctcggg gcacccacct acggccatgt gctgctgggc ttcctggccg
     6361 gggccgcggt ctccgccacc gtggcggtcg tgatcggcgc cttctccctg cgggtcaagg
     6421 cgctgttctt cgccatgatc acgctcgcct tcgcggagtt cgcgctcatc ctcgcggtgc
     6481 agtggagcgc gctcaccggg ggcgaggacg ggctctcgcc gaagctgccg ggtgtgttcg
     6541 cggcgggcgc gtccgggccc ttcgggctca gcggccgact cgtcacctac tacacggtgc
     6601 tcgcggtgtg cgtgggctgc ttcctgatga tgcgccgctt cgtgcactcg ccgctgggcc
     6661 ggacactgca ggccattcgc gacaacgagc tgcgggcgga agcgctcggc taccggacct
     6721 tcgtcttcca gctcatggcc tcgtccttcg gcagtgtggt cgcgaccgtg ctcggcggct
     6781 gctacacgct gtgggtgcgc tacgtgaacc cggagtcggc gctcggcatc ccgatcatgc
     6841 tcgacgtgct gctcatggtg atcatcggcg gcatcggcac cctctacggc ggcatcgtgg
     6901 gggccgccgt cctcctcacc gcgcgcacct tgctgcccaa cctccgcggc ctgggcgcga
     6961 ccgtgttcgc cgggtccgag atcttgcagc ggctctccga gcgctggctg ctctacttcg
     7021 gcatcctctt catcctggtc gtgttcttct ttccccgcgg ggtgctgggg accctccgcg
     7081 aggtagcggc ccggcgacgt cgcgggcgcg tcggggaggc gtgagcgcct ttttcgacgg
     7141 ggttttaggg tatgctgtga ccgagaatgc ccgcccgaga ttacgatgcc atcgtggtcg
     7201 gggccagctt cggcggcctc gccgtcgccc gccagctccg gggagagata ctgctcctgg
     7261 atcgcaacga ggtcggcagc gtgcagacct ccgcgtgcgg caccccgctc tgggttcccg
     7321 aggccctcgg cgtatccgcc agcgtgttgc aggtccacga gcggctcgag atccgcacgc
     7381 ccacccgcac ggttcgctac gatctctccg cggtcccgtt ctgcacgttc gactaccgag
     7441 ccttctgcga ggggctgctg gcccagtgcc gggtccggtt cctgcgtgcc tcggtgaccg
     7501 gcatcgagga tgggacggtc gtgaccgggg cgggacggtt cgccgcgccc gtgatcgtgg
     7561 actgctcggg ctggcggcgc gcgctggtcg gcggcgccga tgctgagccc gcccggcact
     7621 ccttcgggct cgagacccat accacgctcc aggacgaagg cctgaccttc ttgctcgatc
     7681 gccagctgat cccgcaaggg ctgggctgga tcttcccggt cggccacggc agcctgatcg
     7741 gcctcggatc ctatgccggc cggtcgaagc tcaagcccgc gctcgaccgg ctgctgcgcg
     7801 atcacggcac ggcggccggc tcctaccacg gcacctactt ccccaaccgg ccgctccgct
     7861 ccacggtggg gcgcgtgttc gcagtcggcg acgccgccgg gcagtgcctg ccgctcaccg
     7921 cggaaggcat ccgccctgcg ctctacttcg gcggcgagtg cggccgcatc gtccagcgcg
     7981 tgctcgacgg ggccgtgtcg ctcgaggccg gcctcgaggc ctaccagcgg ctcgtcgcgc
     8041 gctaccgccg tccctaccgc gtgctccagc tcgcccagtg gatggccgcc cacacgccca
     8101 cgcgctggtt cgcgatgctc acccagctcg cggccatgca gccgttcctc ccgcgctggt
     8161 ggccgcgcta cgggcggttc ggccggccgt cgggcgtgcc tgcggctacc tgaccgaggg
     8221 gccgagggcg tgacggccgg ggtatcaatg aggatgccgc tggggaggat gccgctgagg
     8281 aagatggcgc tggggacgcc gccgccgccg gggacgatgg gatgagccgg cgcacgcgct
     8341 ggggcatcat catcggagtc gccgggctgt tcatcgccgg ctgcgcgtgg ttcgtgatga
     8401 cgaacgcgcc cctctaccag ctcctcgtcc gcctgtacgt ggacaagatc tatctgaaga
     8461 agatgctgcg cgagtggggc gtcctcgccc cggtcatctt catcgccctc caggccctgc
     8521 aggtcatcgt gtcgccgatc ccgggcgagg cgaccggcat cctcggcggc tatctcttcg
     8581 ggcagtggct ggggctcgtc tactccacca tcggcctcac cctgggctcc gtcggggcct
     8641 tcgccatcgg ccgctggctc ggcgcccact acgtgaagaa cctggtgagc ccggagacct
     8701 ggaaccggct cggcttcatc gtcgaggccg agggcgcggt gctctgcttc atcgtctacc
     8761 tgatcccggg gttgccgaag gacatcgtct gctatctctt cgggatcagt ccgatgccgc
     8821 tctgggtgtt cgcgctggtc tcggggttcg gccgcatccc cggcacctgg gtgctctcgg
     8881 cccagggggc gcataccgcg gccggggact acctgcaggc catcctggtc ggcgcggtgg
     8941 cggtggcggt ggctctgccg ctgtactact accgccaccg gatcatggtc tggttccacg
     9001 gccgcagcgt ggcccgcggt gagggggcca acgccaagct tgacagggag aagccggact
     9061 cgtagactcg gcccactttg gaggacttcg agccagtgcg cacgacggga accgggacga
     9121 cgacgcgccg ccatcatgcg tggcgggcgt atttttactt tcccaccgtc cgcctactgg
     9181 ctcccatgag ctgagcgtaa gggagccacg ggcggacgaa ccggcccgtg gcgccagatc
     9241 gaaagataaa cgccgcgggc caacagcccg cggcgtttct gtttccaagc gccgaaacga
     9301 gtgtccgctg ccagaacgag cgtccgccga ctgggggagt gcggaggcca tgtctgggcc
     9361 gccgcatacg aagagtctaa ggaagtcgac cgcagccaac acgggaggga agacatgatc
     9421 atcgtgctga agtccggtat cagcgacgcc gaggtgaacg acgtgtgccg ccgcatcacc
     9481 gagatgggct acggccccca cgtcatccgc ggtgagttca agaccatcgt ggccgcggtc
     9541 ggcgaggagc gcggccggcc cgacctccgc cagctcgagg cgctggagac ggtcgagtcg
     9601 gtcatgcccg tgcagcagcc gttcaagctg gccagccgcg aggtccgccg cgagtcgagc
     9661 gaggtgcgca tcaacgggat cacggtgggc ggccgcgacg tggtggtgat ggcggggccc
     9721 tgctcggtcg aatccgaggc gcaggtgctc gaggtggcgg aggcggtcaa gggatccggc
     9781 gccaagatcc tgcggggcgg cgcctacaag ccgcgcacct cgccctacgc cttccagggg
     9841 ctgaaggagc aggggctccg ttacctggcg gaggcgcgca agcgcaccgg actgccggtg
     9901 gtcaccgagg tgctcgagac cgagagcgtg gaggtggtgg cggagtacag cgacatcctc
     9961 cagatcgggg cacggaacat ccagaacttc accttgctgc gccgggtcgg cgagatcggc
    10021 aagccggtgc tgctcaagcg gggtatggcg accaccatcc aggagttcct gctctccgcc
    10081 gagtacatcc tctccgcggg caatcccaac gtgatcctct gcgagcgcgg catccgcacc
    10141 ttcgagacct ccacgcggtt cacgctggat ctgaacgcgg tgccggtgct caagaagctc
    10201 tgccacctgc cggtcgtggt ggatccctcg cacggtaccg ggcactggga cctggtggcc
    10261 ccgatggcca aggcctcggt ggcgtgcggg gcggacgggc tcatcatcga ggtgcacccg
    10321 cggccggaag aagcgctctc ggacgggccg cagtcgctca agccgtcgaa gttcgcgcag
    10381 ctcatgtgcg agctccgacc ggtggcggag gcggtggggc gggggctcta gtctcggtca
    10441 ggcggcgttc gtcgcggcgg tgagatcgag cgtctcgcgg acgcgcagga ccagagagct
    10501 tgcgcacgcc ctcctcgtcc tccacgagaa ggatggtctc ggtgcccccc gcggccgcgg
    10561 ccggccgcgc gtccgcttgc tgtccggggt cgtgccaagt gctggagccg gcaaagacgt
    10621 ggaattcttg cgttaagtga cggccgttcc cgatcgcggg cgtggcaagt cgccgctcgg
    10681 tgtcagcagg cggacagaat tcgtcatgga attcgcggag aagagggtgg ggcggctctt
    10741 cccggccggc cccgcggacg gcgggcgggc gtgccataat tgactgcgtg aagtggacga
    10801 ttcggctccc caaggacccg gcccaacgca cgctggctct cgccatcgtg acgtcggtgc
    10861 tgttccacct cttcctggtc gtgcccttca tcgtgatccc cggcttcctg cagccggcgc
    10921 agttcagcaa gcgtggcgag ccgctgctgg tggacatcac ccccgatcgc ccggaggaga
    10981 aggctcccct cggcaacccg tcgcgacccg tgacaccacc tgaggtgcgc gagcgcccgc
    11041 ccagcccgcc ggccaggccg cccgcgccgg cccgcgcggt cgaggcaccc agagcgccgg
    11101 ccccgcgcgc gacaccgccc gcgccgccgc aggccgtcgc gaaggcggag ccaacgcccg
    11161 agcccatggt caagacgccg gagccgcggc cgacggcgcc cgacccgcag ccgccgcaga
    11221 ccgcgccgag ccagcccgcc gtgtcggcgc cgccggcggc gccccaggtc gcggtcgcgc
    11281 gtccgctgga cagcatgcgg cggcagcccg ggggcggcgg ggggctcacg ggtggcggcc
    11341 ggggcggtgt ggagggcgag ccgatcccgc tcgatacccc ggagccgaag tatcaggact
    11401 acttcaacaa gatccgggag cggatcaagg ccaactgggt ctatccgcgc caggccggcg
    11461 agcgcgggat cgagggggag ctgctgatcg agtttcacat cgccaaggac ggccgggtcg
    11521 cgtacatcga gctccgccat tcctccggca ccgcgatcct cgacgatgcc gcgctcaccg
    11581 cggtcaagct ggcgcagccg ttcccgccgg ttcccgacga gatcgccaag cagaccctcg
    11641 cgatcaacgg ccagttcaag taccagatcg tgagcggcct cgtgaaccag ttcctccgtt
    11701 agctccctga cgccgcccgc ccggctcctg tcgctggccc tgctggcgct gatcgtctgc
    11761 gcgggctgtg cccagacgcg ctgcgcggat cgcccgccgg tccgcatcca gctcctgctc
    11821 gtcaacgacg tctacgtgct cgagccggtg gccggccgcg gtgggctcgc ccgcgtggcg
    11881 acgctcgtgc gcgagctgcg ccgccagagc ccccacacgc tcttcgccct ggccggggac
    11941 acgctctcgc cgtccctgct ctcgacgctg cggcgggggg cgcagatgat cgagggctgg
    12001 aacgcggtgg gcctcgacct cgccaccttc ggcaatcacg agttcgattt cggagccgcg
    12061 gtgctgtccg agcgcatgcg ggagtcgcgt ttcccgtggc tgtcggccaa cgtgctcgat
    12121 cgagccaccg gccagccgtt cggcggcgcg cggcgcgggc gcgcgtggga tttcgacggc
    12181 gtgcgtgtcg gggtcatcgg gctcaccacg cccgaggcgg cgcgcacgtc caatcccggc
    12241 ccgggcctgc gcttcgacga cccgctcgcg gcgggccgcg ccgccttcgc cgcgctgggg
    12301 ccgctcgacc tgcgcgtggc ggtgacgcac ctgccgctcc gccaggaccg ggagctggcc
    12361 gccgcgctgg ccgtcgatgc gatcctgggc ggacacgacc acgatgccat gctccacgtg
    12421 gagggccggg cggtgatcgt caaggccggc tccgacgcgc tcaacgtggg ccaggtcgag
    12481 tacgaggtcc gctgcggcgc ggtgatcgcc cgccgccagc gcttgatccc ggtggacgag
    12541 cacctggccg aggccgccga catcgtcgcc ctcgtgcggc agcaggccgc gctgctggcg
    12601 cgcgagctcg acgtggcggt gggccgcacc gcggtggccc tcgacgcgcg cgagagcctg
    12661 acgcgccgcg aggagacgcc gctcgggcag ttcgtcgcgg ccctcctgcg tgatcgggtg
    12721 ggcgcgcagg tggggctgct caacagcggc gccatccgcg gcaaccgggt gatcccggcc
    12781 ggccccctca cgaagaagga cgtccgcgag ctgctgccct tcagcaacac ggtgacgctg
    12841 ctcgaggtga cgggggcggc cctccgggcc gcgctcgagc ggtcggtgga cgagttgcca
    12901 cggcccaccg ggcattttct ccagaccgcc ggcatccaga tgaccgtgga cccggcccgg
    12961 cctcccggcc agcgtgtcgg ggcgatcgtg atcgaggggc ggccgctcga cccggccggg
    13021 ctctaccggg tggcggtgcc cgactacctc gcccgggggc aggacggcta cccgatgctg
    13081 gccgcctccc gggttctcct ggcgccggaa gacggtccgg gcctcatcga gacggtcctg
    13141 gacgggctgg ccgccggccg ttccccttga cggggaaggc cgcggccggt agtgtattca
    13201 atgctttcgg ggcgtaggct cgtagctcag tgggagagcg ctaccttgac acggtagagg
    13261 tcggcggttc gaaaccgccc gagcctacca tttcgtttcg cggcagccct ttgggggtgc
    13321 gcatcgcaag ctggtcgcgc gtgagcgagt gagggagttg cgagcatcac cgggcgcagc
    13381 cctgtgatgc tttgttgtct ctgaaacagg ggaaccatgg ccgaatcgtc tgaagaagcg
    13441 cggcacctgg agttcgcggg ggagttcgac tgcctccccg agcccctgcc cacgctccgc
    13501 cactccacgt cccacgtgat ggcccaggcg gtcaagcggc tcttccccga ggtgaaggtc
    13561 gccatcggcc cggccatcga ggacgggttc tactacgact tcctgaaggc gacgccgttc
    13621 acgccggagg atctcgagcg catcgagcag gtgatgcggg agatcgtcaa ggcggactac
    13681 ccgttcacgc gcgaggagct gccgcgcgac cgggcgatcg ccttcttcga ggagcggggc
    13741 gagcccttca aggtcgagat cctgcgcggg atcgacgcgc ccacggtctc gatctaccgg
    13801 cagggcgact tcctcgacct ctgccgcggg cctcacgtca cgtcgaccgg ccagctcaag
    13861 gccttcaagc tgctctcgag ctccggcgcc tactggcgcg gggacgagaa gaacccgatg
    13921 ctgcagcgga tctacgggac ggcctggctc acccaggagg agctcgacaa gcacgtctgg
    13981 cgcctcgagg aggcgaagaa gcgcgatcac cgcaagctcg gccgcgagct cgacctgttc
    14041 gacttcttcg acatcgcccc gggggcgccg ttctggctgc ccggcggcat gactctcgtc
    14101 cgcgagctgg agaagttcgc ccgggagtcg ctcgacgcgc gcggctacca ggagatcgcc
    14161 accccgctgc tcgtgaacaa gaagctgtgg gagcagtccg ggcactgggc gctctaccag
    14221 gacaacatgt tcaagttcga ggtcgaggac gaggtcttca gcctcaagcc gatgaactgc
    14281 ccggagtccg cccttgtcta caagcgggcg ctccgctcct accgcgatct gcccatccgc
    14341 tactcggaga tggggcgcga ccaccgcaac gagcgctccg gcgcgctgtc cgggctggtg
    14401 cgggtgcgcc agttcaccca ggacgacgcg cacctctact gccggcctga acaggtccag
    14461 tccgagatca cggacctcct ccagctggtg cgcgagtggt acgccacgtt caacctgcag
    14521 ccctcgtacc ggctcgccac gcggcctccc gacaagctcg ggaccgagcg acagtgggac
    14581 caggcggagg acgcgctcca ccaggctctg cgcgcgaaca acctcgtctc cgacgtggac
    14641 aagggcggcg gggccttcta cgggcccaag atcgacatcg acgtggagga cgccctgggg
    14701 cggcagtggc agctcgccac catccaggtg gacctcacga tgctgcccga gcgcatgcag
    14761 tgcgagtaca tcgacacgga cggccaggcc aagcggccgg tggtgatcca ccgcgcgatc
    14821 ttcggctcct acgagcggtt cgtggcgatc ctcaccgagc atttcgcggg cgccttcccg
    14881 acgtggctgg ccccggtgca ggcccgcgtg ctcccgatca gcgaaaaaca cgcggagtat
    14941 ggtcgcaagg tcttcggggc cctccgcgcc gcgcggatcc gcgccgagct cgacgatcgc
    15001 aacgagaagc tcggctaccg catccgcgac gcccagatgc ggaaggtgcc gtacatgctg
    15061 gtcgtgggcg agcgcgaggc ccagagcggc gcggccagcc tgcgtcgccg cacgggcgaa
    15121 gacgtgggcg cggtgccggt cgagcgtatc gtggccgagc tcaccgccga gatcggaagt
    15181 cgttccgcca ccctcaccgt gggtcacgcc ggctgagccg gccccgcacc ccaaggagcg
    15241 cgccatccag tcaaaggaca tccggatcaa tgaaggcatt cgggtgaggg aagtgcgggt
    15301 ggtgtcggcc gacggcgagc agctcgggat cctgccgatc gcgcaggcgc tggagctggc
    15361 ccgccagcgg gagatggacc tggtggaggt ggccgcggag gcccagccgc cggtctgccg
    15421 gatcatggat ttcggcaagt acaagtacat gcaggcgcgg cggcagaagg acgcgcggaa
    15481 gaagcagacg atcatccagg tgaaggaagt gaagctcggc cccaagaccg acacgcacga
    15541 tttcgacttc aaggccaagc acgtgcggcg gttcctggag gaaggcaaca aggccaaggt
    15601 gacggtgcgc ttcaagggcc gcgagatggc ccataccgag ctcggctgga agatgctcaa
    15661 caagatggtg gagggcatgc aggacatcgc ggtcatcgag aaccatcccc ggatggaagg
    15721 ccgcatgctc agcatgatcc tctcgccgaa gccgcactag gggcggacac ccctcaccct
    15781 gccctctccc ctgaggggag agggatataa ggaaggaacg acacttcata tgccgaagat
    15841 gaagacgaag cgggcggcgg ccaagcggct caaggccacc ggcaccggcc ggctcaagcg
    15901 caatcgaggc tggaagcggc acaagctcga ggccaagccg cccaagcgca agcggcgcct
    15961 ggggaaggcg gtcctggtcg cgaaggtgga cgagccgcgt ctcaagcgtc tggttccgta
    16021 cctctaagga gaagaactca tgccacgggc aaagggcggg gccaagaccc gccagcgcag
    16081 gaagaaggtc ctcaagcagg cgaaggggta tttcggcggg cggcgcaagc tcttccgccc
    16141 ggcgtcggag acggtgctgc gcgccggcgc cttcgcctac cgggggcgca agcagaagaa
    16201 gcggcgcgcg cgcgcgctgt ggatcatccg ggtgaacgcg gcgtgccggc aggtcggatt
    16261 gtcctactcc gtcttcatgg cggggctcaa gaaggccggc atcaccctgg atcgcaagat
    16321 cctcgccgag ctggccgtgt ccgatccgac ggccttcgcc aagctggccg agacggctcg
    16381 cgcccagcgc gcctgagcgt ggccggggac ccgcgggtcc aggaagtcct cgaccgggcc
    16441 ctggcggagg tcgccggggc ccgctccacg tccgacctcg agcaggtgcg ggtgcgcgtg
    16501 ctcggccgct cgggccagct caccaccctg ctgcgctcgc tcggctccat cccggccgcg
    16561 gagcggccga agatcggcga gcagaccaac caggccaagg ccagtgtcga ggccgccatc
    16621 gcgcggcggc tcgaaggcct gaaggcggaa gagcgccgcc tcacgcggga ggccgatcgc
    16681 cccgatctca cgctgcccgg tcgccgcccg tccccgggcc cggtgcatcc gatcacccgg
    16741 gtcaccgagg agatcatcga ggtcttcgag gggctggggt tctcggtggc ggaagggccc
    16801 gaggtggagt cggactacta caacttcgcg gcgctgaatt tccccgacga tcatcccgcg
    16861 cgggacatgc aggacacctt tcacatcggg cccgacacgc tcctgcgcac gcacacgtcc
    16921 ccggtgcaga tccggaccat gaaggcgcag cggccgccgg tgcggatcat ctgcctgggc
    16981 aaggtctacc ggcgcgacat cctcgacgcg acccactcgc cgatgttcca ccaggtggag
    17041 gggctggcgg tggacaggca catcacgatg gccgatctga aggccacgct ccagctcttc
    17101 gcgcgggaga tgttcggccc gaaatccgcc gtgcgcttcc gcccgtcgtt cttcccgttc
    17161 accgagccgt ccgccgaggt ggacgtgcgc tgcttcaagt gcggcggcga cggctgccgc
    17221 ttctgcaagg actcgggctg gctcgagatc ctgggctcgg gcatggtgca ccccaacgtg
    17281 ctccgcaacg tgggctacga tccggaagag gtgaccggct gggccttcgg catgggtatc
    17341 gaccgcatcg ccctgctcaa gtacgagatc gacgacatcc ggctcttctt cgacaacgat
    17401 ctccgcttcc tgcagcagtt cgccggcctg cccgggcgat gaagatctcg ttccgctggt
    17461 tgaaggagtt cgtggagacc gagctcgacg cgcgcgcggt ggcggaccgc ctgaccaacg
    17521 cggggatcgg cgtggagcag atcgccccgg tggtggacgg gctggccggc gtggtcgtgg
    17581 gcgagatcga ggcgatcgag cgggacctgg gcgtgagcgc ggccgggcat cacaaccagc
    17641 tcgtgcgggt ggcgctgccc gaccgccgct actcggtggt ctgcggcgcg cccaacgcgg
    17701 cggcgggggc gcgcgcggcc ttcgccccgc cgggggcgcg cctgcccggc ctgggcgagg
    17761 tgaaggcggt caagattcgc ggggtcgtgt cggaagggat cctgtgctcg gagaaggagc
    17821 tgggcatcag cgaggatcac agcgggctcc tgctgctgcc cgcggaggcg ccgctcggcg
    17881 ccgacctcgt gcgatacctg ggcctggacg actggatcct cgatgtcgag atcaccccga
    17941 accggccgga tgcgctctcg gtggtggggg tggcccgcga ggtcgccgcg ctcaccggcg
    18001 ccccgttccg cttccccaag gtgctggtca ccgagggcga gcaggaggcc tccgcgctcg
    18061 ccgtgctcga catccaggcg cccgatctct gccc
//
LOCUS       DASUFA010000186         5763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1167525, whole genome shotgun sequence.
ACCESSION   DASUFA010000186 DASUFA010000000
VERSION     DASUFA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167525"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFX87_06700"
     CDS             <1..687
                     /locus_tag="VFX87_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994637.1"
                     /translation="VTIEGVSKRYGRVAAVSDVSLAVRRGEVLGLLGPNGSGKTTLLR
                     MLTGYLAPTAGRLVVDGFDTERQPMEARRRIGYVPESLPLYGHMRVREFLWFIARLRG
                     LPRKAAVMALDEVIERVRLTDVASAPIRTLSRGYRQRVAIAQGLVHGPEILVLDEPTN
                     GLDPRQIIETRTLIRALAGERTVIMSSHILGEVEKIADRVAILLQGRLLDVRAMADTP
                     DLEGLFLSLT"
     gene            684..1385
                     /locus_tag="VFX87_06705"
     CDS             684..1385
                     /locus_tag="VFX87_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4994638.1"
                     /translation="MSALGILLRKELHATLTSPIAYAVATVFLLVTGYTFSLTLFFTK
                     VANLTYIFHQMYVLLVLLVPVITMRTFAEERKADTLELLLTSPVREVWIVLAKFLAQL
                     TLVVGLLAGSGVYAVILGLYGEPDWAPIYGGYLALLLLGCLLVALGILTSSLTENQVV
                     AATCALGLFLMLWFADSASYMLPAPIDVVAINLSLIGHFTTFVSGSLFLSDAGYYLSL
                     SLLALFLATRVVMDR"
     gene            1396..2877
                     /locus_tag="VFX87_06710"
     CDS             1396..2877
                     /locus_tag="VFX87_06710"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX4994639.1"
                     /translation="MSGLWLGVIAAALVALFAAGTRLPSTRAGWRRVLARGAVVAAAA
                     TVTLLANVALYRHDVHFDITRSHAFTPAPETRRVIDALAEDVDLTYFYQKQNPAGRAA
                     KRMLEIMGRRNPRLHVRTVDPDQYPGLASQYGVRVYNVAVLESGGRRVQVVSTEDRDI
                     ALGLLRLTRSAEKTVCFATGHGEYDIDNLEYHTHFEGVHAHSHGAEGMGVVLMEQHGA
                     GRLRRALESLGFTTRKVVLALESRVPEGCAALVEVNPRTRYAPPESRALEDYLAGGGA
                     ALLLYDVDFPADPTVAATLDKIGIQLGDGVVTDPLDHYFTDPQMVAITRYAPHPVTRG
                     LALSFYPGVRPITLSPPPTGVTVTPLFHSSTESTLRRPGRGGADVNGRRPPGPQTLAV
                     AAEGRWPDRGATAPFRLMVVGDGDFASNSFFPYMSNGDLALSALAWLLREERAPTMRP
                     PVEVLPQVVLTNREVRGIFAFTVLVMPGLAAMLGGAVWWWRRR"
     gene            2874..3407
                     /locus_tag="VFX87_06715"
     CDS             2874..3407
                     /locus_tag="VFX87_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX4994640.1"
                     /translation="MSRVVWIGIALAAGAALTVLAMHGQRPDPAGLARFEAAGVMLAL
                     EPARVTAVDVRQAERRWRFRREPGGGWMADDTAPAPAEITRALEDGLRFLHGSAPQRI
                     LEQTELDGIPMGEYGLAPPRYAVIVQTAGNSSFTIEFGAVNPAGLAQYARVAGRDGIV
                     LLPRFVGRSWETAIGQP"
     gene            3461..4465
                     /locus_tag="VFX87_06720"
     CDS             3461..4465
                     /locus_tag="VFX87_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX4994641.1"
                     /translation="MSVPAGAHTMSTGLATLTVDGRTVSYRLLLVPAELPEEPRRLFA
                     AAAAGDRASAEQVVAILRERLTIRAGEQACRSGRALVQGSGVGDGRLSLELSLDCPGA
                     DRLRIRDDWADVFGSHHQTLARIEVPGGIREIAFLADAREAVIPLAPAAPAGGLGFFR
                     LGIEHILTGYDHLLFLAALLLGGGGALALLKVVTAFTLAHSLTLALAALGLVHVSGRL
                     VEPLIAASIVWVALENIRRRDRVSRRWIVAFGFGLVHGLGFAEALQPLALPPWPLARA
                     LLGFNLGVETGQALVIAVAVPIALWGRRAAWGGRAALTASLAVAVVGTIWLVERVFFA
                     "
     gene            complement(4498..4965)
                     /locus_tag="VFX87_06725"
     CDS             complement(4498..4965)
                     /locus_tag="VFX87_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994642.1"
                     /translation="MGRRGRFGIGALLLAAVIWTGAAQAADSPEPPLYLIPDLHLSIS
                     EVPGEVSVRVPLAVDNHLLYGLLSPYVSLGSSTALGVPWATSLPPGLRRDTDGLEDVR
                     LGAGMAVPVSERVHLYGEYRFLRGRLDAGVGRGLLQREPDSADFRAGFSIRLD"
     gene            complement(4988..5557)
                     /locus_tag="VFX87_06730"
     CDS             complement(4988..5557)
                     /locus_tag="VFX87_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4994643.1"
                     /translation="MTMPPEHAKAKAYLLEKGTQAPVAQIRERVADAFAALESLLDGV
                     TESQARRAPGGGSWSVHEILDHLVATHPLALAEMRALLESRHSPVSPIPAGLQSADPT
                     ARRWDDLRGELRRLHAEVLDTLAKAPDRMTEVRAPIIMVINTRQPDGTVKPMQWVEEC
                     DWKACAIIFRLHELDHLGQARRVLRGLAD"
     gene            complement(5554..>5763)
                     /locus_tag="VFX87_06735"
     CDS             complement(5554..>5763)
                     /locus_tag="VFX87_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994644.1"
                     /translation="RLIGAGLARGVSAGEFRRINVEEAAVVVLAVLDGFSLQLTFDRG
                     LMTVPRAARAAETVLFRYLANGDPT"
BASE COUNT          749 a         2110 c         2025 g          879 t
ORIGIN      
        1 gtgaccatcg agggcgtcag caagcgctat ggccgcgtgg ccgcggtctc cgacgtgagc
       61 ctcgcggttc gccgcggcga agtgctcggg ctgctgggcc cgaacggctc gggcaagacg
      121 acgctcctgc gcatgctgac cggctacctg gccccgacgg cgggtcgcct cgtggtggac
      181 ggtttcgaca ccgagcgcca gcccatggag gcgcggcgcc ggatcggcta cgtcccggaa
      241 tcactcccac tgtacgggca catgcgcgtc cgcgagttct tgtggttcat cgcccggctc
      301 cgcgggctgc cgcggaaagc ggcggtgatg gccctcgacg aagtcatcga gcgcgtgcgg
      361 ctgaccgacg tggcttcggc ccccatccgg acgctctcgc gcggctaccg ccagcgcgtg
      421 gccatcgcgc aggggctcgt gcacggtccc gagatcctcg tgctcgacga gcccaccaac
      481 gggctcgacc cgcggcagat catcgagacc cgcaccctga tccgcgcgct cgccggcgag
      541 cggacggtca tcatgagctc gcacatcctc ggggaggtcg aaaagatcgc cgaccgcgtc
      601 gccatcctcc tgcagggccg gctcctggac gtgcgggcga tggccgacac gccggacctg
      661 gaagggctct tcctgtcgct cacatgagcg cgctcgggat cctgctgcgg aaagagctgc
      721 acgcgaccct cacgtcgccc atcgcgtacg cggtcgccac cgtgttcctg ctcgtgaccg
      781 gttacacgtt cagcctgacc ctcttcttca ccaaggtcgc caacctcacg tacatcttcc
      841 accagatgta cgtcctgctg gtcctgctgg tccccgtcat cacgatgcgc accttcgcgg
      901 aggagcggaa agccgacacc ctcgagctcc tgctgacctc gccggtacgg gaggtgtgga
      961 tcgtcctcgc caagttcctc gcccagctca ccctggtggt cggtctcctc gccggctccg
     1021 gcgtctacgc ggtgatcctg ggcctgtatg gtgagcccga ttgggcgccg atctacggtg
     1081 gctatctcgc gctgctgctc ctgggctgcc tgctcgtggc gctcggcatc ctcacctcga
     1141 gcctcaccga gaaccaggtg gtggccgcga cctgcgcgct cggcctcttc ctcatgctgt
     1201 ggttcgccga ctccgcgtcc tacatgctgc ccgcgccgat cgacgtggtc gccatcaacc
     1261 tgtcgctgat cgggcacttc acgaccttcg tgagcggctc gctgttcctc tccgacgcgg
     1321 gctactacct ctcgctctcg ctcctcgccc tcttcctggc gacccgagtg gtgatggacc
     1381 ggtgaccggg cagtggtgag cggcctctgg ctcggggtga tcgccgccgc gctcgtcgca
     1441 ctcttcgcgg cggggacgcg gctgccctcg acgcgggccg ggtggcggcg ggtgctggcc
     1501 cgcggggcgg tggtggccgc cgcggccacc gtcacccttc tggccaacgt cgcgctctat
     1561 cgccacgacg tgcacttcga catcacccgt tcgcacgcgt tcacgccggc gccggagacc
     1621 cggcgggtga tcgacgcgct cgccgaggac gtggacctga cctacttcta ccagaagcag
     1681 aacccggccg ggcgggccgc caagcggatg ctcgagatca tgggacgccg caatccgcgc
     1741 cttcacgtgc gcacggtcga tccggaccag tacccgggtc tggccagcca gtacggcgtg
     1801 cgggtctaca acgtcgccgt gctcgagagc gggggccgtc gggtacaggt ggtcagcacc
     1861 gaggatcgcg acatcgcgct gggcctcctg cggctgaccc ggtcggccga gaagacggtg
     1921 tgcttcgcca ccggacacgg cgagtacgac atcgacaacc tggagtacca cacgcacttc
     1981 gagggcgtgc acgcgcacag tcacggtgcg gaggggatgg gcgtcgtgct catggagcag
     2041 cacggggcgg gccgcctgcg gcgggcgctc gagagcctcg gcttcacgac acggaaggtg
     2101 gtcctcgcgc tcgagagccg cgtgccggag ggctgcgccg ccctcgtgga ggtcaatccc
     2161 cgcacgcgtt acgcgccacc ggagagccgc gcgctcgagg actacctcgc ggggggcggg
     2221 gccgccctgc tgctgtacga cgtcgacttt ccagccgacc cgaccgtggc cgcgaccctc
     2281 gacaagatcg gcattcagct cggcgacggg gtggtgaccg acccactcga ccactacttc
     2341 accgacccgc agatggtcgc gatcacccgc tacgcgcccc atccggtgac gcgcggcctg
     2401 gccctctcct tctaccccgg cgtgcggccg atcacgctct ccccgccccc caccggggtg
     2461 accgtgacac cgctcttcca cagcagcacc gaatccaccc tccggcgacc gggccggggc
     2521 ggggccgacg tcaacgggcg gcgcccacct gggccgcaga cactcgcggt ggccgcggag
     2581 ggccgatggc cggaccgcgg cgcgaccgcg cccttccgcc tcatggtggt gggcgacggg
     2641 gacttcgcca gcaactcgtt cttcccctac atgtccaacg gcgatctcgc gctgagcgcg
     2701 ctcgcctggc tgttgcgaga ggagcgcgcc cccaccatga gaccgcccgt cgaggtgctg
     2761 cctcaggtcg tgctgaccaa ccgggaggtc cgcgggatct tcgccttcac cgtgctggtc
     2821 atgccgggcc tcgcggcgat gctgggcggt gcggtgtggt ggtggcggcg ccggtgagcc
     2881 gcgtcgtctg gatcggcatc gcgctcgcgg ccggcgccgc cctcacggtc ctggcaatgc
     2941 acgggcagcg acccgacccc gccggcctcg cccgcttcga ggcggcgggt gtcatgctgg
     3001 ccctcgagcc cgcgcgcgtg acggccgtcg acgtgcgcca ggccgagcgg cggtggcgat
     3061 tccggaggga acccggaggc ggatggatgg cggacgatac cgcgccggcg ccggcggaga
     3121 tcacacgggc cctggaagac gggctgcgct tcctccacgg ctccgcgcct cagcggatcc
     3181 tggagcagac cgagctcgat gggatcccga tgggcgagta cggtctggct ccgccgcgct
     3241 acgcggtgat agtccagacc gccggcaact cttccttcac catcgagttc ggcgccgtca
     3301 acccggcggg tctcgcgcag tacgcgcgcg tggccgggcg cgacgggatc gtcctgctgc
     3361 cacgcttcgt cggacggtcg tgggagaccg cgatcggaca gccgtgacgc gtcgggtccc
     3421 ccgggccgtc ctcctggttg ccacgttggc ggcggcgcac atgagcgtgc ccgcgggcgc
     3481 gcacaccatg agcacggggc tcgccacgtt gaccgtggac gggcggacgg tcagctatcg
     3541 cttgctgctc gtgccggccg agctgccgga ggagccgcgc cggctcttcg ccgccgccgc
     3601 ggcgggcgat cgcgccagtg ccgagcaggt cgtggccatc ctgcgcgagc ggctgaccat
     3661 ccgggcgggc gagcaggcct gccggtccgg gcgcgcgctc gtgcaaggct cgggcgtggg
     3721 ggacggccgc ctctcgctcg agctctcgct ggactgcccc ggtgccgacc gtctccgcat
     3781 ccgggacgac tgggccgacg tgttcgggag ccatcatcag accctcgccc gcatcgaggt
     3841 ccccggaggt atccgcgaga tcgcgttcct cgccgacgct cgggaggcgg tcatcccgct
     3901 ggcccccgcg gcgccggccg gcggcctcgg gttcttccgg ctcggcatcg agcacatcct
     3961 caccggctac gaccacctgc tcttcctggc ggccctgctg ctcggcggag gcggtgcgct
     4021 cgcgctcctc aaggtcgtca ccgccttcac gctcgcccac agcctgaccc tggcactcgc
     4081 ggcactcggg ctcgtgcacg tgtcggggcg gctggtcgag ccgctcatcg ccgcctcgat
     4141 cgtctgggtg gcgctcgaga acatccggcg gcgcgaccgc gtgtcgcggc gctggatcgt
     4201 cgccttcggg ttcggcctcg tccacggcct cgggttcgcg gaggcgctcc agcccctcgc
     4261 gctgccgccc tggccgcttg cgcgggccct gctcggcttc aacctgggcg tcgagacggg
     4321 gcaggcgctc gtgatcgcgg tggccgtccc gatcgccctc tgggggcggc gagcggcctg
     4381 gggaggacgc gcggcgctca ccgcgtccct ggcggtggcc gtcgtcggga ccatctggtt
     4441 ggtggagcgg gtcttcttcg cctgatcccg atcgcgtccg gagcgggaag cggggcgcta
     4501 gtcgagccgg atcgagaagc cggcccggaa atccgcggag tcgggctcgc gctggagcag
     4561 gccccgaccg acgcccgcgt cgaggcggcc gcgcaggaag cggtactccc cgtagagatg
     4621 gacgcgctcc gacaccggca ccgccatccc cgcccccagc ctcacgtcct cgagcccgtc
     4681 ggtatcacga cggaggcccg gcggcagcga ggtggcccac ggcacaccca gggccgtact
     4741 cgagcccagc gagacgtacg ggctcaggag cccgtagagc aggtgattgt ccacggccag
     4801 aggcacccgc accgagacct cgcccggcac ctcgctgatc gagaggtgga gatcggggat
     4861 gaggtacagg ggcggctcgg gcgagtccgc ggcctgggcc gcccccgtcc agatgacggc
     4921 ggcgagcagc agcgccccga ttccgaatcg cccccggcgg cccataccgt caagtttata
     4981 cggtgcgtca atctgcgagg ccgcggagaa cccgtctcgc ctggcccagg tgatcgagct
     5041 cgtggagccg gaagatgatg gcgcaggcct tccagtcgca ctcctcgacc cactgcatcg
     5101 gcttcacggt gccgtcgggc tgccgcgtgt tgatcaccat gatgatgggg gcgcggacct
     5161 cggtcatccg gtcgggcgcc ttcgccagcg tgtcgagcac ctccgcgtgg aggcggcgca
     5221 gctcgccccg caggtcgtcc caccgtcgcg cggtgggatc cgccgactgg agcccggccg
     5281 ggatggggct caccggcgag tggcggctct cgagcagcgc gcgcatctcc gcgagcgcca
     5341 ggggatgcgt cgccaccagg tggtccagga tctcgtgcac gctccacgag ccgccgccgg
     5401 gcgcccggcg ggcctggctc tcggtgacgc cgtccagcag gctctccagc gcggcgaacg
     5461 catcggcgac ccgctcgcgg atctgggcaa ccggggcctg cgtgcccttc tcgagcaggt
     5521 aggctttggc cttcgcgtgt tcgggcggca tggtcatgtc gggtctccat tcgcgaggta
     5581 acggaagagc acggtctcgg cggcccgggc cgcgcgcggc accgtcatca atccgcggtc
     5641 gaaggtgagc tggagcgaga agccgtcgag gacggccagc accaccaccg cggcttcctc
     5701 cacgttgatc cggcgaaact cgccggccga gacgccccgg gccaggcccg cgccgatgag
     5761 gcg
//
LOCUS       DASUFA010000187         9028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1411838, whole genome shotgun sequence.
ACCESSION   DASUFA010000187 DASUFA010000000
VERSION     DASUFA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411838"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFX87_06740"
     CDS             <1..1047
                     /locus_tag="VFX87_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HEX4994645.1"
                     /translation="GLVFVGPSWQVIQRMGDKIGARRIMAAAGVPVVPGSDGPVESVD
                     AAREVAARVGYPVMIKAAAGGGGIGMMKVKDEPALSPAFASAQRRAQSAFGSGALFVE
                     RYLEGARHVEVQVFGDATGHVLHLHERECSIQRRHQKLVEESPAPGLAGALKARLTQA
                     ALAGARAVGYVNAGTMEFLVRGDEFYFLEMNTRLQVEHPVTEEVTGLDLVQAQLRVAA
                     GEPLPWTQGQVTQRGSSIECRIYAEDPAKGFMPSPGRLSRLVLPEGPGVRVECGVAEG
                     VEVSVHYDPLLAKLITSGPTRDDAIDRMGAALASFVVEGVKTVIPFHQRVMQSSLFRT
                     GSVHTQMIEQGVFG"
     gene            1110..2711
                     /locus_tag="VFX87_06745"
     CDS             1110..2711
                     /locus_tag="VFX87_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4994646.1"
                     /translation="MTGAGFWRDESLELYLDRWARERPTKTALVDGQSRYTWAALARA
                     VDRVAHGLRAHGVERGSVISCQLPNWNETILIFLAALRLGAVVNPIPPTYRANEIRFM
                     LGLLESQVAVVPAAFRSFSHAGMLASLRPALPRLAHVFVARGQGPEGTVPLATLTDQA
                     WEARVGRAGLPGTDANAVHEVVFTSGTTGEPKGVMHTQNTTLSTIYRAIERLELSDRD
                     VVLMASTLGHQTGYLYGYCLTLLLGATAVWLDVWSAEEGARLIEAEGVTFTMGATPFL
                     RDLTDTAGRRDLGSLRVFISAGAPIPRPLVRDARARLGCAISAGWGMTENGLATCNGL
                     RDAEEKVFGSDGAPLPGMELRVVDGAGTPVPAGCEGDLLVRGAAQFVGYFQRPQFTAD
                     GHTADGWFKTGDRGTLDPAGYLAITGRSKDVIIRGGENIPVVEVENLLYTHPKIAGVA
                     IVAMPDPRLGERACAVVIPREGQTITLGEIVGFLERHELARQKLPERLELVSEFPMTS
                     SGKIQKYRLRELVAERISAGDGRSG"
     gene            2704..3468
                     /locus_tag="VFX87_06750"
     CDS             2704..3468
                     /locus_tag="VFX87_06750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX4994647.1"
                     /translation="MGDRFSLEGRVALVTGAGRGIGRAIAVALGAAGAAVACAARSQD
                     QVDAAAAEITGAGGRACALRLDVTCPEQIVAGVEEVAAALGPVDVLVNNAGVTMEKKT
                     TEVSDEDWDVVLGTNLTSMFRCARAVAPGMMRLERGKIINIGSMYGIIGVPRYAAYCA
                     SKAAVDGLTRSLAAEWARHGIQVNCLAPGYVNTDIPREALADERTRALFLSKIPARRI
                     GEPEEVGALAVYLASPASDFMTGQTVYLDGGQTMAW"
     gene            3488..4285
                     /locus_tag="VFX87_06755"
     CDS             3488..4285
                     /locus_tag="VFX87_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994648.1"
                     /translation="MELNLAGKSVIVTGGGSNIGRAISLAFAREGVHLTVAEIDEGQG
                     RKVVAEAKGLGAASATLVPTDVTSWESVQAMVREVEGRAGRVDVLVNNVGWTYDRLFI
                     EKERSEWEREIQLNLWGMINCTRAVLDGMIARKAGTIVSMGSDAGRMGEFREGVYGAC
                     KAGVIALSKSLAREVGRHGIRLNVVCPGMTVPASEEDFGGLSMWAAEANRAWSAPDMQ
                     ARIAKAYPLRRIGKPEDVAPAVLFLASDAAAFITGQTLSVSGGYTMM"
     gene            4312..5088
                     /locus_tag="VFX87_06760"
     CDS             4312..5088
                     /locus_tag="VFX87_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4994649.1"
                     /translation="MDFQTITYTAGGGVARITLNRPDRLNAISPELLHDLDRACAVVE
                     GDPRARVVTLTAAGRAFCAGADLRAVRELSPDPERWGAFMGLWHRVFDRIEALPVPVI
                     AGVHGLALAGGLELVLVADLVVADDGARLGDQHANFGLVAGGGGSQRLPRLIGARRAK
                     ELMLLGGWLTAPEALAWGLVNRVVPAGAVATAVEEMAAALAAGSGSANRTVKALVARA
                     FDTELAEGLELERRLVAEHMRSADAAEGLRAFAEKRKPVF"
     gene            5097..6248
                     /locus_tag="VFX87_06765"
     CDS             5097..6248
                     /locus_tag="VFX87_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994650.1"
                     /translation="MDLAFSPEQDELVRTLRQFARRELAPRSAHWDRTGEFPWDAWRR
                     MGELGLLGLRVPAAYGGQDADFLTFGIAMEEIGRGDFSCTYGIQLAGLAGEILGRSAT
                     EAIRTRWLPPTASGEAVVALALTEPGAGSDAANLACRAERDGDGYVITGEKSGISLGM
                     AAQAAIVFARTDPAGRARGVTAFLVPLDLPGVSRSPLRDMGTRAIGRAVLAFDHVRLP
                     ASHRLGEEGTGFYQVMQGFDYNRIGIALACLGVAQQSVEETMGYVKERKAFGRALARF
                     EGVSFAIAEAATHLEACRWLCYRALWLADQGRPHTKESAMTKWWGPRLAVDTIHQCLL
                     LHGHYGYTDELPFEQRMRDVIGLEIGDGAAEVMKMVVARELMGRESLPY"
     gene            6309..6524
                     /locus_tag="VFX87_06770"
     CDS             6309..6524
                     /locus_tag="VFX87_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEX4994651.1"
                     /translation="MPEDVKAHITGVVFQITTKPGDAVAAGDAVIVLESMKMEIPVEA
                     PRAGKVREITVAEGQTVQEGDTVAVID"
     gene            6546..7049
                     /locus_tag="VFX87_06775"
     CDS             6546..7049
                     /locus_tag="VFX87_06775"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4994652.1"
                     /translation="MSVALIREHYDYHRWANRRLFDLALALGDGVTRDMGAHWSFPTL
                     KGMFAHIYGADNVWLTRWKGSSPSRLWGDADFPGMGDLRRKWDVLEAEQREFVMGLAE
                     ADLTRPVQYRNTEGKGFQVALGALLLHVVNHATHHRSEAATMITLISGSPPDTGINTY
                     RTAVVKG"
     gene            7061..8605
                     /locus_tag="VFX87_06780"
     CDS             7061..8605
                     /locus_tag="VFX87_06780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX4994653.1"
                     /translation="MKELADELRARRQRAVAMGGPEAVAKQHADGKLTVRERLDLLFD
                     PDSFQEIGVLATHANIVPSMKGRETPADGVVTGFGKVNGRLAAVIAYDFTVMAGSMGR
                     TAEMKCNRAREIALTKRMPMIWLIDSAGARIQEAIGSTFAGSGLLFREESIMSGVVPM
                     VAAMMGPGAAGTAYIPALADFVPMVKGTSHMALGGPPLVKAVVGEDVTAEELGGSKVH
                     TEISGVADLEVADDRACIAAVQEYLSFFPTSNLEQPPVLPSDDPADRMDEALVSLVPD
                     SARRAYDMKKVIEHLVDHGRFFEIKPGWARNLITGLCRLGGRPVGIVANQPMVLGGAL
                     DMDAADKAARFIMLCDAFHISLLFLQDVPGFLVGSKVERQGIIRHGAKMLYAVSEATV
                     PKVTVVVRKAYGAGYFVMCGRAYEPDLIVAWPTAEISVMGPEGGTNIIFRKEIATAAS
                     PDEERARRVEEFRRLINPYIAAGGALIDDVIDPRETRPVVIRALEMARTKKVERPWKK
                     HGVMPV"
     gene            8648..>9028
                     /locus_tag="VFX87_06785"
     CDS             8648..>9028
                     /locus_tag="VFX87_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994654.1"
                     /translation="MHLGICMFATDYAIRTDELARAAEERGFESLFVPEHTHIPTSRR
                     TPFPGGEPLPKEYSHTFDPFISLMAAAAVTKRLKIGTGICLIIERDTITTAKEVASLD
                     LLSGGRFLFGIGAGWNADEMENHGT"
BASE COUNT         1324 a         3065 c         3364 g         1275 t
ORIGIN      
        1 ggcctcgtct tcgtggggcc gtcgtggcag gtgatccagc ggatgggcga caagatcggg
       61 gcgcgccgga tcatggccgc ggcgggcgtg ccggtggtgc cgggcagcga cgggccggtg
      121 gagtcggtgg acgcggcccg cgaggtcgcg gcccgggtcg gctatccggt gatgatcaag
      181 gcggccgcgg gcggcggcgg catcggcatg atgaaggtca aggacgagcc ggccctgagc
      241 cccgccttcg cgtccgcgca gcgccgggcc cagtccgcct tcggctcggg tgcgctcttc
      301 gtcgagcgct acctcgaggg cgcccgtcat gtcgaggtcc aggtcttcgg cgacgcgacc
      361 gggcacgtgc tccacctgca cgagcgcgaa tgctccatcc agcgccggca ccagaagctc
      421 gtcgaagaga gcccggcccc cggcctggcc ggggccctca aggcgcggct cacgcaggcg
      481 gccctggccg gggcgcgcgc ggtcggctac gtcaatgcgg ggacgatgga gttcctcgtc
      541 cgcggggacg agttctactt cctcgagatg aacacccgcc tgcaggtcga gcaccccgtg
      601 accgaggaag tgacggggct cgatctggtg caggcgcagt tgcgggtcgc cgcgggagag
      661 ccgctgccct ggacgcaggg gcaggtgacc cagcgcggct cgagcatcga gtgccgcatc
      721 tacgcggagg acccggccaa gggcttcatg ccgtcgccgg gacgactctc gcgcctcgtc
      781 cttcccgaag gccccggcgt gcgggtcgag tgcggggtcg cggaaggggt cgaggtgtcg
      841 gtgcactacg acccgctcct ggccaagctg atcacctccg ggccgacccg ggacgacgcc
      901 atcgaccgga tgggggcggc cctggcctcg ttcgtggtag agggcgtcaa gacggtgatc
      961 ccgttccatc agcgcgtcat gcagagctcg ctcttccgca ccggctcggt gcacacgcag
     1021 atgatcgagc aaggcgtctt cggctgaggc gcccccggtc aggaccctac gcgatggaga
     1081 cgaacctcac cgagacgagg atccgggcga tgaccggagc ggggttctgg cgcgacgaga
     1141 gcctcgagct ctacctcgat cgctgggccc gtgagcgccc gaccaagacc gcgctcgtcg
     1201 acggtcagag ccgctacacg tgggcggcgc tcgcgcgagc ggtggaccgc gtggcccacg
     1261 gtctgcgcgc gcacggcgtc gagcggggca gcgtcatctc ctgccagctc cccaactgga
     1321 acgagacgat cctgatcttc ctggccgcgc tgaggctggg cgcggtggtc aatccgatcc
     1381 cgccgaccta ccgggccaac gagatccggt tcatgctcgg gttgctggag tcgcaggtgg
     1441 cggtggtgcc ggccgcgttc cgcagcttca gccacgccgg catgcttgcc tcgctccggc
     1501 cggcgctgcc gaggctcgcc cacgtgttcg tcgcccgtgg gcaggggccc gagggcaccg
     1561 tgcccctcgc gacgctcacc gatcaggcct gggaggcgcg ggtcggccgc gccgggctcc
     1621 ccggcacgga cgccaatgcg gtgcatgagg tggtgttcac ctccggcacc accggcgagc
     1681 ccaagggcgt gatgcacacc cagaacacca cgctgtccac catctatcgc gcgatcgagc
     1741 ggctcgagct ctcggaccgc gacgtggtgc tgatggcctc cacgctcggc caccagaccg
     1801 gctatctcta cggctactgc ctgaccttgc tgctcggcgc gaccgcggtc tggctggacg
     1861 tgtggagcgc cgaagaaggg gcgcggctga tcgaggccga gggcgtcacg ttcaccatgg
     1921 gggccacacc gttcctgcgg gacctcaccg acaccgcggg ccggcgtgac ctcggctcgc
     1981 tccgcgtgtt catctcggcg ggcgcgccca tcccgcgccc gctggtgcgg gacgcccggg
     2041 cacgcctggg ctgcgccatc tccgcgggct gggggatgac cgagaatggc ctggccacct
     2101 gcaacggact gcgtgacgcc gaggagaagg tgttcggtag cgacggggcc ccgctgcccg
     2161 ggatggagct ccgggtggtg gatggtgccg gcacgcccgt ccccgccggg tgcgaaggcg
     2221 acctgctggt gcgcggcgcg gcccagttcg tcgggtactt ccagcggccc cagttcaccg
     2281 ctgacggcca caccgccgat ggctggttca agaccggaga ccggggcacc ctcgacccgg
     2341 cgggctacct cgccatcacc gggcgatcga aagacgtcat catccgtggc ggtgagaaca
     2401 tcccggtcgt ggaggtcgag aacttgctct acactcaccc gaagatcgcc ggggtcgcga
     2461 tcgtggccat gcccgacccg cggctggggg agcgggcgtg cgcggtggtg atcccccggg
     2521 aggggcagac gatcaccctc ggcgagatcg tggggttcct ggagcggcac gagctggctc
     2581 ggcagaagct gcccgagcgt ctcgagctcg tctccgagtt cccgatgacg tccagcggga
     2641 agatccagaa gtaccggctc cgcgagctcg tggccgagcg catctcggcc ggagatggcc
     2701 gcagtgggtg accgcttctc cctcgagggc cgcgtcgccc tggtgacggg cgccggccgc
     2761 ggcatcggac gggccatcgc ggtggctctc ggcgcggcgg gagcggcggt ggcctgcgcc
     2821 gcgcgctcgc aggatcaggt ggacgccgcc gccgccgaga tcaccggggc gggcggccgg
     2881 gcttgcgcgc tccggctgga cgtcacgtgt ccggagcaga tcgtggccgg cgtcgaggag
     2941 gtcgcggcgg cgctgggccc cgtcgacgtc ctggtcaaca acgcgggcgt cacgatggag
     3001 aagaagacga cggaggtctc ggacgaggac tgggacgtcg tgctcgggac caacctcacg
     3061 tccatgttcc ggtgcgcgcg ggcggtggcg cccggcatga tgcggctcga gcgaggcaag
     3121 atcatcaaca tcggatccat gtacgggatc atcggggtcc cgcgctacgc ggcgtactgc
     3181 gcgagcaagg cggcggtgga cggtctcacg cggagcctgg ccgcggagtg ggcgcggcac
     3241 ggcatccagg tcaactgtct cgccccgggc tacgtgaaca cggacatccc ccgcgaggcg
     3301 ctggcagacg agaggacgcg cgcgctgttt ctctccaaga tcccggcccg gcggatcggc
     3361 gagccggagg aggtgggcgc cctggccgtc tacctggcct cgcccgcgtc ggacttcatg
     3421 accgggcaga cggtgtacct ggacggcggc cagaccatgg cgtggtgagc atgacgagga
     3481 ggagaggatg gaactgaacc tggcgggcaa gagcgtgatc gtcacgggag gcggctcgaa
     3541 catcgggcgc gcgatcagcc tggccttcgc gcgggagggc gtgcatctca ccgtcgccga
     3601 gatcgacgag ggccagggcc ggaaggtcgt cgctgaggcc aagggcttgg gcgcggcgtc
     3661 cgccaccctc gtgccgaccg acgtgaccag ctgggaatcc gtccaggcca tggtccgcga
     3721 ggtcgagggg cgcgcgggcc gggtggacgt cctggtcaac aacgtgggct ggacctacga
     3781 ccgcctcttc atcgagaagg agcggagcga gtgggagcgg gagatccagc tcaatctctg
     3841 gggcatgatc aactgcaccc gcgcggtgct cgacggcatg atcgcgcgga aggcgggcac
     3901 catcgtgagc atgggctcgg acgccgggcg catgggggag ttccgcgagg gggtctacgg
     3961 cgcgtgcaag gccggcgtca tcgcgctgtc caagagcctc gcgcgcgagg tcggccgcca
     4021 cggcatccgc ctcaacgtgg tctgccccgg catgaccgtc ccggcctcgg aggaagactt
     4081 cggcggcctc agcatgtggg cggcggaggc caaccgcgcc tggagcgcgc cggacatgca
     4141 ggcgcggatc gccaaggcct acccgctgcg gcgcatcggc aagccggagg acgtggcgcc
     4201 cgcggtgctg ttcctcgcct ccgacgccgc ggccttcatc accgggcaga ccctctcggt
     4261 gagcggcggc tataccatga tgtgagcggc tccgctcacc gggagacgac catggacttc
     4321 cagacgatca cctacacggc gggcggcggc gtcgcgcgca tcacgctcaa ccggccggac
     4381 cggctgaacg cgatcagccc cgagctgctc cacgatctcg atcgcgcctg cgcggtggtg
     4441 gagggcgacc cccgcgcgcg cgtggtgacc ctgaccgcgg cgggccgggc cttctgcgcg
     4501 ggcgcggacc tgcgggcggt gcgcgagctc tcgcccgatc ccgagcggtg gggcgccttc
     4561 atgggcctct ggcatcgcgt cttcgaccgg atcgaggccc tgccggtgcc ggtcatcgcg
     4621 ggagtccacg ggctcgcgct ggcgggcggg ctcgagctcg tgctggtggc ggacctcgtg
     4681 gtggccgacg acggggcgcg cctcggcgat cagcacgcga acttcgggct ggtggcgggc
     4741 gggggcggga gccagcgcct gccgcggctc atcggcgcgc gccgggccaa ggagctgatg
     4801 ctgctcggcg gctggctcac cgcgcccgag gcgctggcct ggggcctcgt caatcgcgtc
     4861 gtcccggcgg gggcggtggc gaccgcggtg gaggagatgg cggccgcgct ggccgcgggc
     4921 agcggatcgg ccaaccgcac cgtgaaggcg ctggtggcgc gcgccttcga caccgagctg
     4981 gcggagggcc tcgagctcga gcgccggctg gtggcggagc acatgcgctc ggccgacgcg
     5041 gcggaggggc tgcgtgcgtt cgccgagaag cgcaagccgg tgttctagga ggccgcatgg
     5101 atctcgcgtt ctcgcccgag caggacgagc tggtgcgcac gctccggcag ttcgcccggc
     5161 gagagctcgc gccgcgctcg gcccactggg atcggaccgg cgagtttccc tgggacgcct
     5221 ggcgacgcat gggcgagctg gggctgctgg gcctgcgcgt ccccgcggcc tacggcggcc
     5281 aggacgcgga cttcctgacc ttcggtatcg cgatggagga gatcgggcgc ggggacttca
     5341 gctgcaccta cgggatccag ctcgcgggcc tggccggcga gatcctgggg cgcagcgcga
     5401 ccgaggcgat caggacgcga tggctgcccc cgaccgcgag cggcgaggcg gtggttgcgc
     5461 tcgccctcac cgagcccggc gccggctcgg acgcggccaa cctcgcctgc cgggccgagc
     5521 gcgacggcga cggctacgtc atcaccgggg agaagtccgg catcagcctg ggcatggcgg
     5581 cacaggccgc gatcgtcttc gcgcgcaccg atccggcggg ccgcgcgcgc ggggtgaccg
     5641 ccttcctggt gccgctggac ctgccgggcg tctcgcgcag cccgctgcgc gacatgggta
     5701 cccgcgccat cgggcgggcc gtcctggctt tcgaccacgt gcgtctgccc gcgtcccacc
     5761 ggctcgggga ggagggcacc ggcttctacc aggtcatgca gggcttcgac tacaaccgca
     5821 tcggcatcgc gctcgcctgc ctcggcgtgg cccagcagtc ggtcgaggag accatgggct
     5881 acgtgaagga gcgcaaggcc ttcggccgcg ccctcgcgcg cttcgagggc gtgtccttcg
     5941 cgatcgcgga ggcggcgacg catctcgagg cctgccgctg gctctgctac cgggccctct
     6001 ggctcgcgga ccaggggcgg ccgcacacca aggaatccgc gatgaccaag tggtggggcc
     6061 cgcgcctggc ggtggacacc atccaccagt gcctcctgct ccacggccac tacggctaca
     6121 ccgacgagct gcccttcgag cagcgcatgc gcgacgtgat cgggctcgag atcggcgacg
     6181 gggccgccga ggtcatgaag atggtggtgg cccgcgagct gatgggacgg gagagcctgc
     6241 cctactagcc gcgccgccgg ccgccggccg gatatgagag aatgcggcgg cctcgaaagg
     6301 agcgctcaat gcctgaggac gtcaaagccc atatcaccgg cgtggtgttc cagatcacca
     6361 ccaagccggg cgacgcggtg gccgcgggtg acgcggtcat cgtgctcgaa tccatgaaga
     6421 tggagattcc ggtggaagct ccgcgcgcgg gcaaggtgcg cgagatcacg gtcgccgagg
     6481 gacagacggt gcaggaggga gacaccgtcg cggtcatcga ctgaccccac cgcgagggag
     6541 atgccatgtc cgtcgcgctg atccgggagc actacgacta tcaccgctgg gcgaaccggc
     6601 ggctcttcga cctggctctg gccctgggcg acggggtcac gcgcgacatg ggcgcgcact
     6661 ggagcttccc cacgctcaag ggcatgttcg cccacatcta cggggccgac aacgtctggc
     6721 tcacccgctg gaagggatcg tctccgagcc ggctctgggg cgacgcggac ttcccgggca
     6781 tgggcgatct gcgcaggaag tgggacgtgc tcgaggccga gcagcgcgag ttcgtgatgg
     6841 ggctcgcgga ggccgacctc acccggccgg tgcagtaccg gaacacggag ggcaaggggt
     6901 tccaggtcgc cctcggggcc ctgctgctcc acgtggtcaa ccacgcgacc caccatcgca
     6961 gcgaggccgc gaccatgatc accctgatca gcggttcgcc gcccgacacc gggatcaata
     7021 cctatcgcac cgccgtcgtg aagggctgag atcggtgggg atgaaggagc tggccgacga
     7081 gctccgggcg cggcggcagc gggccgtcgc gatgggaggc ccggaggccg tcgccaagca
     7141 gcacgcggac ggcaagctga ccgtccgcga gcgcctggac ctgctcttcg atccggactc
     7201 gttccaggag atcggcgtgc tggccaccca cgccaatatc gtcccctcca tgaagggccg
     7261 ggagaccccg gctgacggcg tggtcaccgg cttcggcaag gtgaacggcc ggctcgccgc
     7321 cgtgatcgcc tacgacttca cggtcatggc cggctcgatg gggcgcaccg cggagatgaa
     7381 gtgcaaccgg gcgcgcgaga tcgcgctcac caagcgcatg ccgatgatct ggctgatcga
     7441 ctcggcaggt gcgcgcatcc aggaggcgat cggctccacg ttcgccgggt cgggcttgct
     7501 gttccgcgaa gaatccatca tgtcgggcgt cgtcccgatg gtggcggcca tgatggggcc
     7561 gggcgcggcc ggcaccgcgt acatcccggc gctcgccgac ttcgtgccga tggtgaaagg
     7621 gacgagccac atggctctgg gcggcccccc gctggtgaag gcggtggtgg gggaggacgt
     7681 cacggcggag gagctgggcg gctcgaaagt gcacaccgag atctcgggcg tggccgacct
     7741 cgaggtcgcg gacgaccggg cctgcatcgc cgcggtgcag gagtatctct ccttcttccc
     7801 gacgtcgaac ctggagcagc cgccggtgct gccctcggac gatccggccg atcggatgga
     7861 cgaggcgctg gtcagcctcg tgccggacag cgcgcgccgc gcctacgaca tgaagaaggt
     7921 gatcgagcac ctggtcgatc acggccgctt cttcgagatc aagccgggct gggcccgcaa
     7981 cctgatcacc gggctctgcc gcctgggcgg ccggccggtg gggatcgtcg cgaaccagcc
     8041 catggtgctg ggcggggccc tcgacatgga cgccgcggac aaggcggcgc gcttcatcat
     8101 gctctgcgac gccttccaca tctcgctgct gttcctgcag gacgtgcccg ggttcctggt
     8161 gggctcgaag gtggagcggc agggcatcat ccgccacgga gccaagatgc tctacgcggt
     8221 cagcgaggcc accgtgccga aggtcacggt cgtggtgcgc aaggcctacg gcgcgggcta
     8281 cttcgtcatg tgcgggcgcg cctacgagcc ggacctgatc gtggcctggc ccaccgccga
     8341 gatctcggtg atggggccgg agggcggcac caacatcatc ttccggaagg agatcgcgac
     8401 cgcggccagc ccggatgaag agcgcgcccg ccgggtggag gagttccgtc ggctgatcaa
     8461 tccctacatc gcggccggcg gagcgctgat cgacgacgtc atcgacccgc gggaaacccg
     8521 cccggtggtg attcgcgcgc tcgagatggc gcgcaccaag aaggtcgagc ggccctggaa
     8581 gaagcacgga gtgatgccgg tctagcccaa ccgcggcccg ccagaggcgg gcataaggag
     8641 gtttgccatg catttgggta tctgcatgtt cgccaccgac tacgccatcc ggaccgacga
     8701 gctggcccgt gccgccgagg agcgcggctt cgagtcgctg ttcgtgccgg agcataccca
     8761 catccccacc agccggcgca ccccgttccc gggcggcgag ccgctcccca aggagtactc
     8821 ccacacgttc gatccgttca tctcgctgat ggcggcggcc gcggtcacca agcgcctcaa
     8881 gatcggcacc ggcatctgcc tgatcatcga gcgcgacacc atcaccaccg ccaaggaggt
     8941 ggcgagcctc gacctgctct cgggcgggcg cttcctcttc gggatcggcg cgggctggaa
     9001 cgccgatgag atggagaacc acggcacc
//
LOCUS       DASUFA010000188        16139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_616283, whole genome shotgun sequence.
ACCESSION   DASUFA010000188 DASUFA010000000
VERSION     DASUFA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 16139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16139
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616283"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VFX87_06790"
     CDS             <1..1432
                     /locus_tag="VFX87_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994655.1"
                     /translation="HEVAYESLRPERRRQLHARIVLAIEAGHSDRPLEDVDRLAHHAT
                     RGELWEKAVEYSRQAGAKAAARSAHLQAVAYLEQALDALAHLPEERAQVEQAIDVRLD
                     LRNSLHPLGDPERILALLREAEALARTLDDPRRLAQVFSFLTQHFRLIGHLDLAVESA
                     ERALALADRLRDTPFWIVANTFLGTAYDARGEYRKAADVLRKSVEALSGSSTGQDVRV
                     AGLVSVFSRIYLVYCLADMGDFREGLAYGDEGTRLAEAADDAYSLIFASCGVGTLHLL
                     KGDVEPAIRTLERGLNLYRTWIVPVALPLIACALGAAYSLSGRSHDAIALLEEGTREG
                     LAIGRMGGHSLLVVRLGEAYLRAGRLDEADETARRALLMAREKNERGHEAYALHLLGE
                     VVARRTPGGAPAADAALRQASALAEELSMRPLLAHCHVSRARLECRAGRTASARQLFD
                     AGTAAFREMEMPFWLERAEAEARSLA"
     gene            complement(1560..1757)
                     /locus_tag="VFX87_06795"
     CDS             complement(1560..1757)
                     /locus_tag="VFX87_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994656.1"
                     /translation="MMIQVSQTTRKGRTTIRIAATPKSAQTRALLAQFKKGVKAIEKK
                     WKAHAAAAKKRAAKAGRRRRR"
     gene            complement(1754..2875)
                     /locus_tag="VFX87_06800"
     CDS             complement(1754..2875)
                     /locus_tag="VFX87_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994657.1"
                     /translation="MNARVLVTSAGSGASGNLVRSLRAGNAALGIVGCHDDQFILKNS
                     TADRNYLVPPLAHPAWSRSLRRIVETEQIDLVVPASDGDVAALSRARATLGGRLFLPR
                     AATVERCGDKYGLAEWLRARGVPAPATYRVTDLRYLERIFERLGNPSRAWCRIRAGAG
                     SRGAAPVAGPEQARSWITYWRDMRSVPVSAFTLSEYLPGRDFGCQSVWKDGRLALAKT
                     YERLSYLGSGSQPSEVSAVGALTKTVHEPGVLEIAVRAIRAVDRKASGVFAVDLKEDS
                     RRVPRVTEINAGRFTSSTNLLDLTGKHNMTAVYLDLARGEPVDLHEEYEGTEHHYMLR
                     DIDAVPRVFDADEFFEGVEDARRPRRAQRGNGHPEEPTP"
     gene            complement(3059..3547)
                     /locus_tag="VFX87_06805"
     CDS             complement(3059..3547)
                     /locus_tag="VFX87_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX4994658.1"
                     /translation="MLAGCASAPADRGPLSAEAILKDKDGKQVGVATFIETPEGVRLA
                     VTGYRLPPGGHGLHIHAVGRCEPPEFTSAGGHFNPTSKQHGRMNPAGPHAGDLPNLVV
                     AASGEGGIDITTKAFTLSPGPNSVLGDKPTALVVHANPDDDKTDPTGNSGGRIACGVI
                     TK"
     gene            complement(3599..7078)
                     /locus_tag="VFX87_06810"
     CDS             complement(3599..7078)
                     /locus_tag="VFX87_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX4994659.1"
                     /translation="MTTPTGEFSLEAKYTQERGRVYLSGIQALVRLPLDQHRADIRRG
                     LRTATFISGYRGSPLGGLDQTLERMTPLLRAHDVVFSSGLNEDLGATAVYGSQMAGLF
                     PKPRYDGVLGMWYGKAPGVDRSGDIFKHANYAGVGKNGGVLALAGDDPLSKSSTLPSH
                     SEVALYDALMPTIYPGNVQEILDLGLHGFMLSRVSGLWVGAKIVTNVADEAGTAEVDP
                     ERVSPIIPVVELDGKPFQHQINVNLIPPYGLDMERTIHLARLELARRYAYENKLNRIT
                     VPSPNAWFGIVTSGKTYYDVRQALAELGLDDGALRRYGVRILKMGMLFPMEPRIVREF
                     GRGLQEILVVEEKRSFLEMFCKDVLYGLTDRPQVVGKLDEEDRPLLSPVGELDSDSIA
                     RAIARRLARRVKIDSVEARIQHLDELKRRPKTLTLARSAFFCSGCPHNRSTVVPEGSV
                     AAAGIGCHGMAMGMDRGIIGVTHMGAEGAQWVGIAPFTETPHLFQNIGDGTFFHSGGL
                     ALNYAVASGVNITYKLLYNGAVAMTGGQNAAGALPIPALSRRLEAEGVKRIIITSDDP
                     AKYSGVSIAGNAEVWHRDRLLEAQSVLAAISGVTVLIHDQHCAAEKRRLRKRGKMVDP
                     QTRVFINERVCEGCGDCGKKSNCLSVQPIDTEFGRKTAIHQSSCNKDYSCLLGDCPSF
                     LTIEGAGTAPKRERRLTPLDAELPEPALKVPSEGFALHMMGIGGTGVVTMNQILGTAA
                     LLDGRQVRGLDQTGLSQKGGPVVSDLKLSSEPIERANKVSAGSADLYLGFDLLVATDP
                     GNLDKAESGRTIAVVSTSQIPTGQMVVDTAVHFPELSSMLMSIDRVTRKDPNVYLDAQ
                     AIAEALFGDHMAVNPIMLGAAFQAGALPISAAAIERAIRINGVSVDMNVLAFRWGRMA
                     VVDRKQVEAAVAQATAQVVEAPRVLSAEARALVDRVEATGELRRLLEVRVPELIAYQN
                     AAYAGQYVDFVRRVAEVEVERAPGSVGLAEAVARHLYKLMAYKDEYEVARLHLDAALA
                     AQLEAKFGQTIRTYWHLHPPLLRALGLKKKIRLGAWFAPAFQLLKAMKGLRGTALDPF
                     GYAEVRRVERALIGEYRQLIETALVRLSPVTHGAAVTLAELPDEIRGYEHIKLEGVAR
                     FREKAKQLTAQLN"
     gene            complement(7266..7901)
                     /locus_tag="VFX87_06815"
     CDS             complement(7266..7901)
                     /locus_tag="VFX87_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX4994660.1"
                     /translation="MHALLGGLLLLVQLAVVACAPAPAAAQAQDRVIVAFGDSLTSGL
                     GVPVDQTYPALLGERLRREGYAYKVVNAGVSGDTTAGGLRRVDWALRLKPEIVILELG
                     VNDALRGQNLASVRANMDQLVARFQAAGARVLVAGMRLPPNYGTPYADDFYRLFNEVA
                     RARNAPYLPFFLEGVGAIPRLNQADGIHPTAEGYALVVEHLWPFLVPLLRK"
     gene            7948..8958
                     /locus_tag="VFX87_06820"
     CDS             7948..8958
                     /locus_tag="VFX87_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HEX4994661.1"
                     /translation="MHTIRRSTIHLLLLSGVLAPAPAAALGPEQTALIVNTADPYSME
                     IGAYYARRRGIPRGNVIFVTLPVGRDEISRQEFESAKAEVDARLPATVQALVLAWTTP
                     FRVDCLSITTAFAFGFDAAFCAQGCRPTRRSPYFNSPSRAPFTDLALRPAMLLAGQSV
                     AEGKALIDRGVAADATFPGGTAYLLSTSDRARNARAAGYALLDGVKPPVRIERIDADE
                     LRDRRDVLFYFTGLSAVAGLETLRFRPGAIADHLTSTGGVLTGHAQMSALRWLEAGAT
                     ASYGTVVEPCNFATKFPQPALAIARYTRGETVLEAYWKSVAMPGQGVFVGEPLAAPFR
                     AR"
     gene            8987..9652
                     /locus_tag="VFX87_06825"
     CDS             8987..9652
                     /locus_tag="VFX87_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994662.1"
                     /translation="MIRVRGLSMRLASGGRGVDVLTDVSLDVPAGQFLAIAGPSGSGK
                     STLLGLIAGLDQPTAGRIEVAGVDITALDEDALARFRRDHVGYVFQSFHLLPTLTAQE
                     NVAVPLELAGEAGAADRAAALLAEVGLAERAHHYPVQLSGGEQQRVAVARAMARRPTL
                     LLADEPTGNLDSATGKQIIELLVGMNRRLGSTLVLVTHDTALAAHADRVVTLRDGRIV
                     SDE"
     gene            9649..12132
                     /locus_tag="VFX87_06830"
     CDS             9649..12132
                     /locus_tag="VFX87_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4994663.1"
                     /translation="MSRFVLRLAWRETRGAWRHFGYFFACITLGVSALVGVGSFADSL
                     ERTVARSAKSLMGGDVEVRSSQPLPGDAAALIAGVATTRVREMVAMAQAGPSQSHIVE
                     LKAVEPGYPFYGALVTEPARPLETLIGGGRALVHDALLARFGLRLGDRFRVGDLELTI
                     TGVIQKEPDRAIGVFSLGPRVLIAGDDLDRTGLIRPGSRVRYRTLVRLPEGRDAQAFR
                     DRLAAALPAGQRVSTFGQAQPGLRRFWDQLTMYLGLTGLVALMVGGIGVAVSVNAFVR
                     QKLASIAILKALGAGWRPLLATYLLQTALLGLGGSALGALLGSAVQPLLAPTLTRLLP
                     IELTLSFSFRAVLSGLAMGVGVTLLYALWPLLQIRHVRPALILRMDIEPRLRGRRPWA
                     TALPLAAGLAGLALWQAGSWKIGALFAGGLAAALVLLALGARLVTALARRVRWRSPAW
                     RQGAANLHRPGSHAGPVLVSLGLAVMLVVSIALLDGSLRAQLVDRAPGSAPAFFFIDI
                     QTDQAEPFARLVAAHGASAPAELTPVVRSRLAAVNGASIEGRTRREDAWYLTREYVLT
                     WAKEPPGHNTVVAGRWWTPEEAAREPLISVEEEIARQLGVGLGDTLTFDIQGVPVSAR
                     VTSLRHVDWRSLTSNFFVIFSPGALDGAPSTFIATVGAPPEQETRLQSAVVAAFPNIT
                     AIPIREVLERVGAVLDQIALAVRLVAAFSIGAGLIVMAGALAITRQQRLYQSVILKAL
                     GATRGFVSRVFAVEYALLGAAAGLAGSALAAGLAWVVLRFALEVPWRWAPGTLLAGVA
                     TATALALLVGFLGTRRLLGRRPLGVLRSE"
     gene            12129..12932
                     /locus_tag="VFX87_06835"
     CDS             12129..12932
                     /locus_tag="VFX87_06835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX4994664.1"
                     /translation="MTLLERLRALYPEASGRSLKQWLEAGRVEVNGRPARDGRLALAA
                     GDIVALAARRAAPFPRGLSLVHEDDAILVVVKPSGLLSIATDRERERTAYRLLWDYLA
                     AKRQRPFIVHRLDRETSGLLVFAKSEETKRALQAQFEARNVERVYLALVEGGPPDERG
                     TLESKLAEDRMLRVRSAEKGRQAITHYRVLRRGREGCLLELRLGTGRRHQIRVQLAEA
                     GWPIVGDVAHGGARGSVGRLCLHATRLGFVHPVTQRQVVFESAPPADWV"
     gene            13031..13828
                     /locus_tag="VFX87_06840"
     CDS             13031..13828
                     /locus_tag="VFX87_06840"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006673180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4994665.1"
                     /translation="MAASPFSIEDKVAIVTGGGVGIGKSIALEFARAGADVVIASRKL
                     ENLEPVTAEIRKLGRRSFCVALDVRQEDQVKGLVERTVKEMGRLDVMVNNAGASFRAK
                     VEDISANGWNTVVGINLNGTFFGCKWAGKQMMDQPSGGSIVNISSIAGVYGSTMMSHY
                     GASKAAVINFTRALGMAWGRKGVRVNCIAPGPVETEGYLGVLTKQDPAAAKKAYDTVA
                     NRVAMGRWGKVEEIAYPCIFLASAASSFMTGETIVIDGGPAPQLGDG"
     gene            13847..14734
                     /locus_tag="VFX87_06845"
     CDS             13847..14734
                     /locus_tag="VFX87_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX4994666.1"
                     /translation="MPELPEVEAARRAAHKVAAGRRIVEAHVADDPIVFEGVAPARFR
                     RALVGRLVKAVRRHGKHLWLELDRRPWPCLHFGMTGGIHVTRANPGGAARRAAVPAGR
                     AVRLVSDGRVDRPDWPPRFTKLHLRFAGGGELALADGRRLGRIRLRRDPPTEPPISAL
                     GFDAWRAMPTPAQFHAMLRERNAPIKAVLLDQAFAAGIGNWIADEVLYQAGIAPRRRA
                     STLSAAEAGRLRTRIRSVIGGAVRAGSDSDRFPRWWLFHRRWGHRRGGPSATTVRGEH
                     IRHETVGGRTTAWVPTAQR"
     gene            14776..15963
                     /locus_tag="VFX87_06850"
     CDS             14776..15963
                     /locus_tag="VFX87_06850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX4994667.1"
                     /translation="MREAVVVASSRTPLAKSHRGSFNITRPDDLAAHCIKDVLGKTPQ
                     LDPAEIEDVILGCAQPNGAQGNNVARVALVRAGLPVSIAGTTVNRFCSSGLQAIAMAA
                     HQIILGGAEAAIGGGLESISMMKRDGDPNPWVKDKKPGIYMVMGDTAEVVAKRYNITR
                     QMQDEYSLVSQQRTARAQQEGFFKEELAPLQVVRGIVDKKTGEITGKEDFYVDKDECN
                     RPDTTLEGLLALPPHFDKTSGKGTVSAGNSSQLSDGASATLLMSADRAKALGIKPKLI
                     FRGFAVAGCEPDEMGIGPVYAVPKLLKRNGLKMDDIGVWELNEAFASQVVYCRDKLGI
                     PMEKLNPNGGAISIGHPFGMTGSRMVGTIANEMLRRKARYGVVTMCIGGGQGAAGLFE
                     SCL"
BASE COUNT         2194 a         5849 c         5698 g         2398 t
ORIGIN      
        1 ccacgaggtc gcctacgaga gcctccgacc ggagcgccgc cggcagctcc acgcccgcat
       61 cgtcctcgcc atcgaggccg gtcactccga ccgcccgctc gaggacgtcg atcggctcgc
      121 ccatcacgcg acgagagggg agctctggga gaaggcggtg gagtactcgc gtcaggcggg
      181 cgccaaggcc gccgcgcggt ccgctcacct gcaggcggtc gcatatctcg agcaggcgct
      241 ggacgccctc gcgcatctgc ccgaggagcg cgcgcaggtc gagcaggcca tcgatgtccg
      301 gctcgacctc cgcaactccc tgcacccgct gggagacccc gagcggatcc tcgcgctgct
      361 gcgggaggcc gaagcgctcg cccggacgct cgacgatcca cgacggctcg cccaggtctt
      421 ctcgttcctg acgcaacatt tccggctgat cggacatctc gatctcgcgg tggagtcggc
      481 ggagcgcgcg ctcgcgctcg ccgaccgact gcgggacact cccttctgga tcgtcgccaa
      541 cacgttcctg ggcaccgctt acgatgcccg aggcgagtac cgcaaggcgg ccgacgtcct
      601 ccggaagagc gtggaggccc tgtccggcag ctcgaccggc caggacgtgc gcgtcgccgg
      661 cctcgtgtcg gtcttctccc gtatctacct ggtctactgc ctcgccgaca tgggtgactt
      721 ccgcgagggg ctggcgtacg gggacgaggg cacccggctc gccgaggccg ccgatgatgc
      781 gtacagcctc atcttcgcct cgtgcggcgt cggcacgctc cacctcctga agggcgatgt
      841 ggagccggcg atccgtaccc tggagcgcgg gctcaacctc taccggacgt ggatcgtgcc
      901 ggtggcgctg ccgctgatcg cgtgcgcgct gggcgccgcc tactccctgt ccgggcgatc
      961 gcacgacgcg atcgccctgc tggaggaggg gacccgagaa ggcctcgcga tcgggaggat
     1021 gggcgggcac tctctgctcg tcgtccgcct gggcgaggcc tatctccggg ccggccgcct
     1081 cgacgaggcc gacgaaacgg cccgacgggc cctgctgatg gcccgcgaga agaacgagcg
     1141 cgggcacgaa gcctacgccc tgcacctcct gggcgaggtc gtggcgcgac ggacgccggg
     1201 gggcgcgccc gccgccgatg ccgcgcttcg acaggcctcg gccctcgccg aggagctctc
     1261 gatgcgcccg ctcctggcgc actgccacgt gagccgcgcc cggctcgagt gccgggcggg
     1321 ccggaccgcg agcgcccggc agctcttcga cgcgggcacc gcggccttcc gcgagatgga
     1381 gatgccgttc tggctcgagc gggcggaggc cgaggcccgc tcgctagcct gacctcccct
     1441 tctcgcgcgt ctccacctgg atgtccacga ggtccaggcc gcacgcccgg gccagccgcc
     1501 tgatctcgag gcgaaccgcc tccagcgtgt gcgcgccgat cgggccgtcc agctcgatgc
     1561 taccgacgac ggcggcgtcc ggccttggcg gcccgcttct tggcggcggc ggcgtgcgcc
     1621 ttccacttct tctcaatggc cttcactccc ttcttgaact gcgcgaggag ggctcgcgtc
     1681 tgggcgctct tgggcgtcgc cgcgatccgg atggtggtac ggcccttcct ggtcgtctgg
     1741 ctcacctgaa tcatcatggg gtcggctcct ctgggtgtcc gttgccgcgt tgcgctcgcc
     1801 tcgggcggcg ggcgtcctcc actccctcga agaactcatc ggcgtcaaac acgcgcggga
     1861 cggcgtcgat gtcccgcagc atgtagtggt gctcggtccc ctcgtactcc tcgtggagat
     1921 ccaccggctc gccccgggcg agatcgaggt agaccgcggt catgttgtgc ttgccggtca
     1981 ggtcgaggag gttggtgctg gacgtgaacc ggccggcgtt gatctcggtg acacgcggaa
     2041 cccggcgcga gtcctccttc agatcgaccg cgaacacgcc cgatgccttc cgatccaccg
     2101 cccggatcgc ccggaccgcg atctcgagca cgcccggctc gtggacggtc ttggtgagcg
     2161 cgcccaccgc cgacacctcg ctcggctggc tcccgctgcc gaggtacgac aggcgctcgt
     2221 acgtcttggc cagcgccagg cgaccgtcct tccacacgct ctggcagccg aaatcgcggc
     2281 ccggcaggta ctcggacagg gtgaaggcgg agacgggcac gcttcgcatg tcgcgccagt
     2341 acgtgatcca gctgcgtgcc tgctccggcc cggcgaccgg ggcggcgccc cgcgagcccg
     2401 cgcccgcccg gatgcgacac cacgcgcggg acggattccc caggcgctcg aagatgcgct
     2461 cgagataccg caagtcagtc acacgatagg tcgccggcgc gggaacaccg cgggcccgca
     2521 gccactcggc gagcccgtac ttgtcgccgc agcgctccac cgtcgcggcc cggggaagga
     2581 agagacggcc gccgagcgtg gcccgcgcgc gcgagagcgc ggcgacgtcc ccgtcactgg
     2641 ccgggacgac gaggtcgatc tgctccgtct ccacgatccg gcgcagcgag cggctccacg
     2701 ccgggtgcgc gaggggcggg acgaggtagt tgcggtccgc cgtggagttc ttgaggatga
     2761 actggtcgtc gtggcagccc acgatgccga gcgcggcatt ccccgcccgg aggctccgca
     2821 cgaggttgcc gctcgccccg ctgcccgcgc tcgtgaccag tacccgcgcg ttcatcggcg
     2881 ccgaccctag acgatatccg gagccaccgc aagtttcccg cgcggcccgc gcgcgcgctg
     2941 tgcttactgg gtccgggcac ttgcgcgagt gacttcacgc ggttctcagc ccgccggaaa
     3001 agcaaacggg ccgggccccg aaggacccgg cccgcgtgcg ccgcgcgctg gcccgcgact
     3061 atttcgtgat gacgccgcag gcgatgcgac cgccgctgtt gccggtggga tcggtcttgt
     3121 cgtcgtccgg attggcgtgg acgacgagcg cggtcggctt gtcgccgagc accgagttgg
     3181 gcccggggct cagcgtgaag gccttggtgg tgatgtcgat gccgccctcg cccgaggccg
     3241 ccaccaccag gttgggcagg tcgcccgcgt gcggacccgc cgggttcatg cggccgtgct
     3301 gcttgctcgt cgggttgaag tgcccgcccg ccgacgtgaa ctcgggcggc tcgcaccgcc
     3361 ccaccgcgtg gatgtgcagc ccgtgcccgc ccggaggaag ccggtagccg gtcaccgcca
     3421 gccgcacgcc ctccggtgtc tcgatgaacg tcgccacgcc cacctgcttg ccgtccttgt
     3481 ccttgaggat cgcctcggcc gagagcggtc cccggtcggc cggcgcgctc gcgcatccgg
     3541 ctagcattgc ggccacagcg agcagcagta gtggtctcac cgttgagtgc cctcccgttc
     3601 agttgagctg cgcagtgagc tgcttggctt tctcccggaa ccgcgccacg ccctcgagct
     3661 tgatgtgctc gtagccgcgg atctcgtcgg gcagctcggc cagggttacc gccgcgccgt
     3721 gagtgacggg gctgagccgc acgagcgcgg tctcgatgag ctgccggtat tcgccgatca
     3781 gcgcccgctc cacccgccgc acctccgcgt agccgaaggg atcgagcgcg gtgccccgca
     3841 gccccttcat ggccttcaag agctggaagg cgggggcgaa ccaggcgccg agccggatct
     3901 tcttcttgag gcccagcgcg cggagcagcg gcgggtgcag atgccagtag gtccggatcg
     3961 tctggccgaa cttcgcctca agctgcgcgg ccagcgccgc gtcgaggtgc aggcgcgcca
     4021 cctcgtactc gtccttgtag gccatcagct tgtagaggtg ccgcgcgacc gcctccgcga
     4081 gccccacgct gcccggggcg cgctccacct ccacctcggc cacgcggcgg acgaagtcca
     4141 cgtactggcc cgcgtaggcc gcgttctggt aggcgatcag ctcgggcacg cgaacctcga
     4201 gcaggcgccg gagctccccc gtcgcctcca cgcgatccac gagggcgcgc gcctccgcgc
     4261 tcagcacgcg gggcgcctcg acgacctgcg cggtggcctg ggcgaccgcc gcctccacct
     4321 gcttgcggtc caccaccgcc atccgccccc accggaaggc gagcacgttc atgtccaccg
     4381 agacgccgtt gatccggatc gcgcgctcga tggccgcggc cgagatcggc agcgcgcccg
     4441 cctggaaggc cgcccccagc atgatcgggt tcaccgccat gtggtcgccg aagagcgcct
     4501 ccgcgatggc ctgggcgtcg aggtacacgt tggggtcctt ccgcgtcacc cgatcgatgc
     4561 tcatcagcat gctgctgagc tcggggaagt gcaccgcggt gtccaccacc atctggccgg
     4621 tgggaatctg gctggtggac accaccgcga tcgtgcggcc cgactccgcc ttgtcgaggt
     4681 tgcccgggtc ggtcgccacc aggaggtcga agcccaggta gagatcggcg gaccccgcgg
     4741 agaccttgtt ggcgcgctcg atcggctcgg agctgagctt caggtcggag accaccggcc
     4801 cgcccttctg gctgagcccg gtctggtcga gcccgcgcac ctgccggccg tcgagcagcg
     4861 ccgcggtgcc gaggatctga ttcatagtga cgaccccggt gccgccgatg cccatcatgt
     4921 gcagcgcgaa gccctcgctc ggcaccttca acgcgggctc ggggagttcg gcgtcgagcg
     4981 gggtcagcct ccgttcccgc ttcggcgccg tcccggcgcc ctcgatggtg aggaacgacg
     5041 ggcagtcgcc gagcaggcac gagtagtcct tgttgcagga cgactggtgg atcgcggtct
     5101 ttcgtccgaa ctcggtgtcg atcggctgca cggacaggca gttcgacttc ttgccgcagt
     5161 cgccgcaccc ctcgcacacg cgctcgttga tgaagacgcg ggtctgcggg tccaccatct
     5221 tgccgcgctt gcgcagccgc cgcttctccg ccgcgcagtg ctggtcgtgg atcagcacgg
     5281 tgacgccgct gatggccgcg agcacagact gcgcctccag cagccggtcg cgatgccaga
     5341 cctccgcgtt gcccgcgatc gacacccccg agtacttggc gggatcgtcg ctggtgatga
     5401 tgatgcgctt gaccccctcc gcctcgaggc gccggctgag cgcgggaatg ggcagggccc
     5461 ccgccgcgtt ctggccgccg gtcatcgcca ccgcgccgtt gtagaggagc ttgtaggtga
     5521 tgttcacgcc ggaggccacc gcgtagttca gggcgaggcc gcccgagtgg aagaaggtcc
     5581 cgtcgccgat gttctggaag agatggggcg tctcggtgaa cggcgcgatg ccgacccact
     5641 gcgcgccctc cgcgcccatg tgggtcaccc cgatgatgcc gcggtccatc cccatcgcca
     5701 tgccgtggca gccgatgccc gcggcggcca cgctgccctc gggcactacg gtcgagcggt
     5761 tgtgcgggca gcccgagcag aagaaggcgg agcgggccag ggtcagcgtc ttgggccggc
     5821 gcttgagctc gtcgaggtgc tggatgcgcg cctccaccga gtcgatcttg acgcggcggg
     5881 cgaggcgccg ggcgatggcc cgcgcgatgg aatcggaatc gagctcgccg accggcgata
     5941 gcagcggccg gtcctcctcg tcgagcttgc ccaccacctg cggccggtcg gtcaggccgt
     6001 agaggacgtc cttgcagaac atctcgagga acgagcgctt ctcctcgacg acgaggatct
     6061 cctgcaggcc gcgcccgaac tcgcgcacga tgcgcggctc catcgggaac agcatgccca
     6121 tcttgaggat gcggacgccg tagcgccgca gcgctccgtc gtcgagcccg agctcggcga
     6181 gggcctgccg gacgtcgtag taggtcttgc cgctcgtgac gatgccgaac cacgcgttcg
     6241 gcgacggcac cgtgatccgg ttgagcttgt tctcgtacgc gtagcgtcgg gcgagctcga
     6301 ggcgcgcgag atgaatcgtg cgctccatgt cgaggccgta gggcgggatc aggttcacgt
     6361 tgatctggtg ctggaagggc ttgccgtcca gctcgactac cgggatgatc gggctcaccc
     6421 gctccggatc cacctcggcg gtccccgcct cgtccgccac gttcgtgacg atcttggcgc
     6481 ccacccagag acccgacacc cgcgagagca tgaagccgtg caggcccagg tcgaggatct
     6541 cctgcacgtt gcccgggtag atggtgggca tcagcgcgtc gtagagcgcg acctcggagt
     6601 ggctgggcag agtggacgat ttggaaagcg gatcatcccc ggccagcgcg agcacgccgc
     6661 cgttcttgcc cacgcccgcg tagttggcgt gcttgaagat gtcgccgctg cggtccaccc
     6721 ccggggcctt gccgtaccac atgccgagca cgccgtcgta ccggggcttc ggaaagagtc
     6781 ccgccatctg gctgccgtac accgcggtgg cgcccagatc ctcgttgagc ccggaggaga
     6841 atacgacgtc gtgggcccgg agcagcgggg tcatccgctc gagggtctgg tcgagccccc
     6901 cgaggggcga gccgcggtag cccgagatga acgtcgccgt cctgaggcct cgccggatgt
     6961 cggcgcgatg ctgatcgagg gggagccgga ccagcgcctg gatgccggac aggtagacgc
     7021 ggccgcgctc ctgggtgtac ttggcctcga gcgagaactc tccggtcggt gtggtcatga
     7081 gctccccttg tggggtggcg acgttagaca tcgaacgacg gccatatagt agtcgggaaa
     7141 ggcatttcga gcaataggtg gaggcgcgca tactcgttta gttccttaag ctctattgag
     7201 tgcgggggcc cagaaatggc ccccgcactc ccccagtcgg cggacgcccg tttcggcgct
     7261 ttcgactatt tgcgcagcag gggcacgagg aacggccaca ggtgctcgac cacgagtgca
     7321 tacccctcgg ccgtcgggtg gatcccgtcc gcctggttga gccgcgggat cgcgccgacg
     7381 ccctcgagaa agaacggcag atacggcgcg ttgcgcgcgc gcgccacctc gttgaagagc
     7441 cggtagaagt cgtcggcgta gggggtgccg tagttcgggg gcaggcgcat gcccgccacg
     7501 agcacgcggg cgccggcggc ctggaagcgc gccacgagct gatccatgtt ggcccgcacc
     7561 gaggccaggt tctggccgcg gagggcgtcg ttgacgccca gctccaagat cacgatctcg
     7621 ggcttgaggc gcagcgccca gtccacgcgc cggagcccgc ccgcggtggt gtcgcccgag
     7681 acgcccgcgt tgacgacctt gtacgcgtag ccctcgcgcc ggagccgctc gccgagcagc
     7741 gcgggatagg tctgatcgac cggcacgccg aggcccgagg tgaggctgtc gccgaaggcg
     7801 acgatcacac ggtcctgcgc ctgtgcggcg gccggcgcgg gcgcgcatgc gacgaccgcg
     7861 agctggacca gcaggaggag ccccccgagc agcgcgtgca tgtccctagt gtagtatggg
     7921 cggcgagcgc cccggcgcgg accgcacgtg cacacgatcc ggagatcgac gattcacctg
     7981 ctcctcctgt cgggcgtcct cgcgcctgcc ccggcggcgg ccctcggtcc cgagcagacc
     8041 gcgctgatcg tcaacacggc ggacccgtac agcatggaga tcggggccta ctacgcgaga
     8101 cggcgaggca tcccgcgcgg caacgtgatc ttcgtcacgt tgccggtggg ccgggacgag
     8161 atcagccggc aagaattcga gagcgcgaag gcagaggtag acgcccgact ccccgcgacg
     8221 gttcaggctc tggtcctcgc ctggaccaca ccctttcggg tggactgcct gtcgatcacc
     8281 acggcgttcg ccttcggctt cgacgcggcc ttctgcgctc agggctgccg gcccacgcga
     8341 cgaagtcctt acttcaactc gccgagccgt gccccgttca ccgatcttgc gctccggccc
     8401 gccatgctcc tggccggcca gagcgtggcg gaaggcaagg ccttgatcga ccgcggcgtt
     8461 gcggccgacg ccacgttccc gggcgggacc gcgtacctgc tctccacgtc ggaccgcgcc
     8521 cggaacgcgc gcgcggcagg ttacgcgctg ctcgacgggg tcaagccccc cgtgcggatc
     8581 gagcggatcg acgcggacga gctgcgggat cgccgcgacg tgctgttcta cttcaccggt
     8641 ctctcggcgg tcgcagggct cgagacgctt cgcttccggc ccggcgccat cgccgaccac
     8701 ctgacctcga ccggcggcgt gctgaccggc cacgcgcaga tgagcgcgct ccggtggctc
     8761 gaggcgggcg cgaccgccag ctacggcacc gtggtggagc cgtgcaactt cgcgacgaag
     8821 ttcccgcagc cggccctggc gatcgcacgg tacacccgcg gggagaccgt gctcgaggcg
     8881 tactggaaga gcgtggccat gcccggccag ggcgtgttcg tcggcgagcc cctcgccgcg
     8941 ccgttccgcg cgcgctagcc gttcggcgtg tagtatggac ggcatcatga tccgcgtccg
     9001 cgggctctcc atgcgcctcg cgagcggcgg gcgcggcgtg gacgtgctca ccgacgtatc
     9061 cctcgacgtg cccgccggcc agttcctcgc catcgccggc ccctcgggca gcggcaagtc
     9121 cacgctgctc gggctcatcg ccgggctcga tcagccgacc gccggccgga tcgaggtggc
     9181 gggcgtggat atcaccgcgc tcgacgagga cgccctcgcg cgctttcgcc gcgaccacgt
     9241 gggctacgtg ttccagtcct tccatctcct ccccacgctc accgcgcagg agaatgtcgc
     9301 ggtcccgctc gagctggccg gtgaagccgg cgcggccgac cgcgcggcgg cgctgctcgc
     9361 cgaggtgggc ctggccgagc gcgcgcacca ctacccggtg cagctctcgg gcggcgagca
     9421 gcagcgcgtc gcggtggccc gcgcgatggc gcgccggccc acgctgctcc tggccgacga
     9481 gccgaccggc aatctcgatt cggccaccgg caagcagatc atcgagctgc tggtgggcat
     9541 gaaccgccgc ctcggcagca cgctcgtgct ggtcactcac gacacggcgc tggccgccca
     9601 cgccgaccgc gtggtcacgc tgcgcgacgg ccgcatcgtg agcgacgaat gagccggttc
     9661 gtcctccgcc tcgcctggcg ggagacacgg ggggcgtggc ggcacttcgg ctacttcttc
     9721 gcctgcatca cgctgggcgt cagcgcgctg gtcggggtgg gcagcttcgc ggatagcctc
     9781 gagcgcaccg tcgcccgctc ggccaagtcg ctgatgggcg gcgacgtgga ggtccggagc
     9841 tcccagcctc tgcccggaga cgccgccgcc ctcatcgcgg gggtggcgac cacgcgcgtg
     9901 cgcgagatgg tggccatggc ccaggccggg ccgtcgcaga gccatatcgt cgagctcaag
     9961 gccgtcgagc cgggctatcc cttctacggc gcgctcgtga cggagccggc gcgcccgctc
    10021 gagacgctga tcggcggcgg ccgcgcgctc gtgcacgacg ccctcctcgc ccgcttcggg
    10081 ctccggctgg gcgaccgctt ccgcgtgggc gacctcgagc tgaccatcac cggcgtcatc
    10141 cagaaggagc cggaccgcgc gatcggcgtc ttctcgctgg gcccgcgagt gctgatcgcc
    10201 ggtgacgatc tcgaccggac cgggctgatc cgccccggca gccgcgtgcg ctaccgcacg
    10261 ctcgtccgcc tgcccgaggg ccgcgacgcc caggccttcc gcgaccggct cgcggcggcg
    10321 ctgcccgccg gacagcgcgt atcgaccttc ggtcaggccc agcccggcct gcggcgcttc
    10381 tgggaccagc tcacgatgta tctggggctc accgggctcg tggcgctgat ggtgggcggt
    10441 atcggggtgg cggtgagcgt gaacgccttc gtgcggcaga agctggcgtc gatcgccatc
    10501 ctgaaggcgc tcggcgccgg ctggcggccg ctgctcgcca cgtacctgct gcagaccgcc
    10561 ctgctcgggc tgggcggcag cgcgctcggc gcgctcctgg gcagcgcggt ccagccgctg
    10621 ctcgcgccca ccctcacgcg cctgctgccg atcgagctca cgctgtcctt ctcgttccgc
    10681 gcggtgctga gcggtctcgc catgggcgtg ggcgtgaccc tcctctacgc gctctggccg
    10741 ctgttgcaga tccggcacgt ccggcctgcc ctgatcttgc gaatggacat cgagccgcgc
    10801 ctgcgcggcc gccggccgtg ggccaccgcg ctcccgctcg cggccggcct cgcggggctc
    10861 gcgctctggc aggccggctc ctggaagatc ggcgcgctgt tcgcgggcgg gctcgccgcc
    10921 gccctcgtgc tcctggccct cggcgcccgc ctcgtcaccg cgctggcccg ccgtgtgcgc
    10981 tggcggtctc cggcctggcg gcagggcgcc gccaatctcc accgccccgg cagccacgcg
    11041 ggtcccgtgc tggtctcgct cgggctggcc gtgatgctcg tcgtctccat cgccctgctc
    11101 gacgggagcc tgcgcgcgca gctcgtggac cgcgctcccg gcagcgcacc cgcgttcttc
    11161 ttcatcgaca tccagaccga ccaggccgag cccttcgcgc ggctcgtggc ggcccacggc
    11221 gcgagcgcgc ccgccgagct gaccccggtg gtgcgctcgc gcctggccgc ggtcaacggc
    11281 gcctcgatcg aagggcgcac cagacgggaa gacgcgtggt acctcacgcg cgagtacgtg
    11341 ctgacctggg cgaaggagcc gcccgggcac aacacggtcg tggccggccg ctggtggacg
    11401 cccgaggaag cggcccgcga gcccctcatc tcggtcgagg aggagatcgc gcgccagctc
    11461 ggggtggggc tcggcgacac gctcaccttc gacatccagg gggtgccggt gagcgcgcgg
    11521 gtcacgagcc tgcgccacgt ggactggcgc tcgctcacct ccaacttctt cgtgatcttc
    11581 tccccgggcg cgctggatgg ggcgccgagc accttcatcg ccaccgtggg agcgcctccc
    11641 gagcaggaga cccggctcca gtccgcggtg gtggccgcct tcccgaacat caccgcgatc
    11701 ccgatccggg aagtgctcga gcgcgtcggc gccgtgctcg accagatcgc gctcgcggtg
    11761 cggctggtcg ccgccttcag catcggggcc gggctcatcg tgatggcggg cgcgctcgcg
    11821 atcacccggc agcagcggct gtaccagtcg gtgatcctga aggcgctcgg cgcgacccgc
    11881 ggcttcgtct cccgcgtgtt cgcggtggag tacgcgctcc tcggcgcggc ggccgggctg
    11941 gcgggcagcg ccctcgccgc gggcctggcg tgggtggtgc tccgcttcgc gctcgaggtg
    12001 ccctggcgct gggcccccgg cacgctgctg gccggcgtgg cgaccgccac cgccctcgcg
    12061 ctgctggtgg gcttcctcgg cacccggcgc ctgctcggcc gacgcccgct cggcgtgctc
    12121 cgaagcgagt gacgctgctc gagcggctgc gcgcgctcta cccggaggcc tcgggccgga
    12181 gcctcaagca gtggctcgag gcggggcgcg tggaggtcaa cgggcgcccg gcccgtgacg
    12241 gccggctcgc cctggccgcc ggcgacatcg tcgcgctcgc cgcgcgccgc gccgcgccct
    12301 tcccgcgtgg cctctcgctc gtgcacgagg acgacgcgat cctcgtcgtg gtcaagccgt
    12361 ctggcctcct ctcgatcgcc accgatcgcg agcgggagcg caccgcgtac cgcctgctct
    12421 gggactacct cgcggccaag cggcagcggc cgttcatcgt ccaccggctc gatcgcgaga
    12481 cgtccgggct gctcgtcttc gccaagtccg aggagacgaa gcgggcgctc caggcgcagt
    12541 tcgaggcgcg caacgtggag cgcgtgtacc tggcgctggt cgagggcggg ccgcccgacg
    12601 agcggggcac gctggagagc aagctcgcgg aggaccgcat gctgcgggtg cgctcggccg
    12661 agaagggccg ccaggccatc acccactacc gcgtgctgag gcgggggcgc gagggctgcc
    12721 tgctcgagct gcgcctcggc accgggcgcc ggcaccagat ccgcgtgcag ctcgccgagg
    12781 cgggctggcc gatcgtcggc gacgtggccc acggcggcgc ccggggcagt gtcgggcggc
    12841 tgtgcctgca cgccacgcgg ctcggcttcg tgcatccggt gacgcagcgg caggtcgtct
    12901 tcgagagcgc gccgcccgcc gactgggtat aatccgcgcg gtcgcggatc gtctctcctc
    12961 accctgccct ctccccagag gggagaggag atcggatcac ggagggagag ggagaagccc
    13021 cggaggccac atggctgcgt cgccgttttc catcgaggac aaggtcgcca tcgtcaccgg
    13081 cggcggagtc ggcatcggca agtccatcgc gctcgagttc gcccgcgcgg gcgccgacgt
    13141 ggtgatcgcg agccgcaagc tcgagaacct cgagccggtc accgccgaga tccggaagct
    13201 gggccgccgc tccttctgcg tggcgctcga cgtccgccag gaggaccagg tcaagggcct
    13261 ggtcgagcgc accgtgaagg agatggggcg cctcgacgtc atggtcaaca acgcgggcgc
    13321 ctcgttccgg gccaaggtcg aggacatctc ggccaacggc tggaacacgg tggtcggcat
    13381 caacctgaac ggcaccttct tcggctgcaa gtgggcgggc aagcagatga tggaccagcc
    13441 gagcggcggc tccatcgtca acatctcgtc catcgcgggc gtctacggct cgacgatgat
    13501 gtcgcactac ggcgcctcca aggccgcggt gatcaacttc acccgcgcgc tcggcatggc
    13561 gtgggggcgc aagggcgtgc gcgtgaactg catcgccccc ggcccggtgg agaccgaggg
    13621 ctacctgggc gtgctcacca agcaggaccc ggccgcggcc aagaaggcct acgacacggt
    13681 ggccaatcgc gtggccatgg gtcgctgggg caaggtggaa gagatcgcgt atccgtgcat
    13741 cttcctggcc tccgcggcgt cgtcgttcat gaccggcgag accatcgtca tcgacggcgg
    13801 acccgcgccg cagctcggcg acggctgagc gccggccgat cagagcatgc ccgagctgcc
    13861 ggaggtcgag gcggcgcggc gcgcggccca caaggtggcg gccgggcgcc ggatcgtcga
    13921 ggcccacgtc gccgacgacc cgatcgtctt cgaaggcgtc gcgcccgccc gctttcgccg
    13981 ggccctcgtc ggccgcctcg tgaaggcggt gcgacgccac ggcaagcacc tctggctcga
    14041 gctggaccgg cgcccgtggc cgtgtcttca cttcggcatg accggcggca tccatgtcac
    14101 ccgagcgaac ccgggcggcg ctgcgcgccg cgcggctgtg ccggcgggac gggcggtgcg
    14161 cctggtgtcc gacggtcgtg tggaccggcc ggactggccg ccgcgcttca ccaagctcca
    14221 tctgcgcttc gcggggggcg gcgagctggc gctggcggac ggccgccggc tcggccgcat
    14281 ccggctgcgc cgggacccgc ccaccgagcc gccgatcagc gcgctcggct tcgatgcctg
    14341 gcgggccatg ccgacccccg cgcaattcca cgcgatgctc cgcgagcgca atgcgccgat
    14401 caaggcggtg ctcctcgacc aggcgttcgc ggcgggcatc ggtaactgga tcgccgacga
    14461 ggtgctctac caggccggga tcgcccccag gcgccgcgca agcacgctgt ccgccgcgga
    14521 ggccgggcgg ctgcggaccc ggatccgctc ggtgatcggc ggggcggtgc gggcgggctc
    14581 ggacagcgac cgcttcccgc gctggtggct cttccatcgc cgctggggcc accgccgcgg
    14641 gggcccgtcc gccaccaccg tgcggggcga gcacatccgc cacgagacgg tcgggggccg
    14701 caccaccgcg tgggtgccca cggcgcagcg ctgaggtata ctccccggtg acttccccct
    14761 gagaaaggag cccccatgag agaggctgtc gtcgtcgcca gctcccgcac cccgctcgcc
    14821 aagtcccatc gcgggtcgtt caacatcacc cgcccggatg acctggccgc gcactgcatc
    14881 aaggacgtcc tcggcaagac cccgcagctc gatccggccg agatcgagga cgtgatcctg
    14941 ggctgcgccc agcccaacgg cgcgcagggc aacaacgtcg cgcgcgtggc gctggtccgg
    15001 gcgggcctgc ccgtctcgat cgcgggcacg acggtcaacc gcttctgctc ctcggggctg
    15061 caggccatcg cgatggccgc gcaccagatc atcctgggcg gcgcggaggc ggccatcggc
    15121 ggcgggctcg agtccatcag catgatgaag cgcgacggcg atccgaaccc gtgggtgaag
    15181 gacaagaagc ccggcatcta catggtgatg ggcgacaccg ccgaggtggt ggccaagcgc
    15241 tacaacatca cccgccagat gcaggacgag tactccctgg tgagccagca gcgcaccgcg
    15301 cgggcccagc aggagggctt cttcaaggaa gagctggccc cgctccaggt cgtgcgcggc
    15361 atcgtggaca agaagaccgg cgagatcacc ggcaaggaag acttctacgt ggacaaggac
    15421 gagtgcaacc gtcccgacac cacgctcgaa ggcctgctcg cgctcccgcc gcacttcgac
    15481 aagacgagcg gcaagggcac cgtcagcgcg ggcaactcct cgcagctctc cgacggcgcc
    15541 tccgcgacgc tcctgatgtc ggcggaccgg gccaaggcgc tcggcatcaa gcccaagctg
    15601 atcttccgcg gcttcgcggt ggcgggctgc gagccggacg agatgggcat cgggcccgtc
    15661 tacgcggtgc cgaagctgct caagcgcaac ggcctcaaga tggacgacat cggcgtgtgg
    15721 gagctgaacg aggccttcgc ctcgcaggtg gtctactgcc gcgacaagct gggcatcccg
    15781 atggagaagc tcaacccgaa cggcggcgcg atctcgatcg gccacccctt cggcatgacc
    15841 ggctcgcgca tggtcggcac catcgccaac gagatgctgc ggcgcaaggc gcgctacggc
    15901 gtcgtgacca tgtgcatcgg cggcggccag ggcgcggccg gcctcttcga gtcctgtctg
    15961 taatctcctc gcggtgatct catcccctcg ccctcaaccc tctcccctcc ggggagaggg
    16021 cagggtgagg ggcgaattca acgcgtcgcg actagagaca ggactcgaag aggccggccg
    16081 cgccctggcc gccgccgatg cacatggtca cgacgccgta gcgcgccttg cgccgcagc
//
LOCUS       DASUFA010000189         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1630722, whole genome shotgun sequence.
ACCESSION   DASUFA010000189 DASUFA010000000
VERSION     DASUFA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630722"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFX87_06855"
     CDS             <1..882
                     /locus_tag="VFX87_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4994668.1"
                     /translation="GKQVSQLKQARKPVPAALGVQYRIADKLVFSKMQAALGGRLRFA
                     VSGGAPLSREIAEFFHAAGILILEGYGLTETCPVLTNNREDKFKFGSVGLPLPGVEIR
                     IAPDGEILGRGANIAQGYFKKPEATAEVFLAGGWFATGDIGRIDEDGFLFITDRKKDL
                     IVTAGGMNIAPQNIENLLKGDPFVSQVMVYGDRRPFPVALITLNPDELAAFAKAQGIL
                     TSDPVALAKHPKVLERVSRIVEERNSELQSYAKIKKFTILPTDFTVENGYLTPTLKVK
                     RKVIRDKYQDTIDQLYR"
     gene            886..1647
                     /locus_tag="VFX87_06860"
     CDS             886..1647
                     /locus_tag="VFX87_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994669.1"
                     /translation="MSEPLRLEGKVAIVTGASRGLGRAMALALAEAGAHVAVAARTTA
                     EIDETAHAVAQRGRRAIAVPTDVSDYAQVEALVARTADALGGLHILVNNSGIAPVHGV
                     AEMPPEEFARTLQVNLVGVFNGCRAAASRLIGQGSGKVINMASVMAVSGLAGYSAYAA
                     SKGGVMSFTKCLAVEWARHNIQVNAIAPGWFVTDMNAMAFADPKVRDRLLRDVPMRRT
                     GELAEIGPLVVYLASSDSSYMTGQTLFLDGGHSAA"
     gene            complement(1659..2930)
                     /locus_tag="VFX87_06865"
     CDS             complement(1659..2930)
                     /locus_tag="VFX87_06865"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX4994670.1"
                     /translation="MLQPDRPPRQRRLALFVLLLAVGLALAALPFRATWWGGWILAIA
                     EAGIVGGLADWFAVTAIFRRPLGLPIPHTALIPANWELMAKRVGSMVGDRVLTKTYVL
                     DEVARFDVADLIARAAERLEREDVEAAVRAVTRWAAGELSPSASTDLVAWLRRLLSAQ
                     PVAPLLAGAIEVARRQGWDQRAIEALARALAEALDRPDFRAAVGELVDGVLGDYRAKM
                     GAYPRLLLGLADVLGLIDRDRLVGALHGAVRKIARDPQDPLRRRLEESIAALPGRLRD
                     EPAFAARVEAAKEALVASPEVARLLEDAVEGVRRALVADLAGERSEVVPWITDRLDRA
                     RRQLAADAALRQALDRWVKARAGELIERYHDHIARFIERGVRALGPEGAVRLIEEHAG
                     DDLQYIRVNGTVVGGLAGGGLYALHLLLGLF"
BASE COUNT          488 a         1099 c         1074 g          411 t
ORIGIN      
        1 gggaagcagg tctcccagct caagcaggcc agaaagcccg tgcccgccgc gctcggcgtc
       61 cagtaccgta tcgccgacaa gctcgtcttc tcgaagatgc aggccgctct cggcgggcgg
      121 ctgcgcttcg cagtctccgg cggcgccccg ctgtcccggg agatcgccga gttcttccac
      181 gctgccggca tcctcatcct cgagggctac gggctcaccg agacctgccc cgtgctcacc
      241 aacaaccgcg aggacaagtt caagttcggc tcggtgggct tgccgctgcc gggggtggag
      301 atccgcatcg cgcccgatgg ggagatcctg ggacggggcg ccaatatcgc ccagggttac
      361 ttcaagaagc cggaggccac cgccgaggtg tttctggccg gaggctggtt cgccaccggg
      421 gacatcgggc ggatcgacga ggacggcttc ctcttcatca ccgaccggaa gaaggacctc
      481 atcgtcaccg cgggggggat gaacatcgcc ccccagaaca tcgagaatct gctcaagggc
      541 gatcccttcg tcagccaggt catggtctac ggcgaccggc gtccgttccc ggtggcgctc
      601 atcacgctga acccggacga gctggccgcc ttcgccaagg cgcagggcat cctgacgtcg
      661 gaccccgtcg cgctcgcaaa gcatcccaag gtgctggagc gggtctcccg catcgtggag
      721 gagcgcaact ccgagctgca gtcctacgcc aagatcaaga agttcaccat cctgcccacc
      781 gatttcacgg tggagaacgg ctacctcacc ccgacgctga aggtgaagcg caaggtgatc
      841 cgcgacaagt accaggacac catcgaccag ctctaccgct gagacatgtc ggaaccgctc
      901 cggctcgagg gcaaggtcgc gatcgtcacc ggagcgagcc ggggcctggg ccgcgccatg
      961 gcgctggccc tggcggaagc gggggcgcac gtggcggtgg cggcgcggac cacggccgag
     1021 atcgacgaga ccgcgcacgc ggtggcgcag cgcgggcggc gcgccatcgc ggtgcccacc
     1081 gacgtgtcgg actacgcgca ggtggaagcg ctggtggcgc ggacggcgga cgcgctcggg
     1141 ggactgcaca tcctggtcaa caattcgggc atcgcccccg tccacggggt ggcggagatg
     1201 ccgccggaag aattcgcgcg cacgctccag gtcaacctgg tgggcgtctt caacggctgc
     1261 cgggccgccg cgtcccggct gatcgggcaa gggtcgggca aggtgatcaa catggcctcg
     1321 gtgatggcgg tgtcggggct cgccggctac tccgcctatg cggcgtccaa gggcggcgtg
     1381 atgtcgttca ccaagtgcct ggcggtggaa tgggcgcgcc acaacatcca ggtcaatgcc
     1441 atcgcgccgg gctggtttgt caccgacatg aacgcgatgg ccttcgccga tcccaaggtg
     1501 cgggaccggc tgctccgcga cgtgcccatg cgccgcacgg gcgagctggc ggagatcggc
     1561 ccgctggtcg tctacctcgc ctcgtcggat tcctcctaca tgaccggaca gaccctcttc
     1621 ctcgacggcg ggcatagcgc cgcctgagcg cacggccctc agaagagacc gagcagaagg
     1681 tgcagggcgt acagcccacc gccggcaagc ccgcccacca cggtgccgtt gacgcggatg
     1741 tactggaggt cgtcgccggc atgctcctcg atgaggcgca ccgcgccttc cggtcccagc
     1801 gcgcgcacgc cccgctcgat gaaccgcgcg atgtggtcgt ggtagcgctc gatgagctcg
     1861 ccggcgcggg ccttgaccca gcggtccagc gcctgccgca gcgccgcgtc ggcggcgagc
     1921 tgccggcgcg cgcgatcgag ccggtcggtg atccagggca cgacctccga ccgctcgccg
     1981 gcgagatcgg cgacgagcgc gcgccgcacg ccctcgacgg cgtcttccag aagccgcgcc
     2041 acctccggac tcgccaccag ggcctccttg gcggcctcca cgcgcgccgc gaaggccggc
     2101 tcgtcgcgga gccggcccgg cagcgcggcg atggactcct cgagccgccg ccgcagcgga
     2161 tcctggggat cgcgggcgat tttccggacc gcgccgtgta gcgcgcccac cagacggtcg
     2221 cgatcgatga ggccgagcac gtcggccagc cccagcagca ggcggggata ggctcccatc
     2281 ttcgcccggt agtcgccgag cacgccgtcc accagctcgc ccaccgcggc gcggaagtcg
     2341 ggccggtcca gcgcctccgc gagcgcccgc gccagcgcct cgatggcgcg ctggtcccag
     2401 ccctggcgcc gggccacctc gatggctccc gcgaggagcg gcgccacggg ctgcgccgag
     2461 agcaggcgcc ggagccaggc gaccaggtcg gtcgaggccg agggcgacag ctcgcccgcc
     2521 gcccagcggg tgacggcgcg caccgcggcc tccacgtcct cgcgctcgag ccgctcggcg
     2581 gcgcgcgcga tgagatcggc gacgtcgaag cgcgccacct cgtcgagcac gtaggtcttg
     2641 gtgagcacgc ggtcgccgac catgctgccc acgcgcttcg ccatcagctc ccagttggcg
     2701 gggatgagcg cggtgtgggg gatgggcagc cccagcgggc gccggaagat cgccgtgacc
     2761 gcgaaccagt ccgcgagccc gcccacgatc ccggcctcgg cgatggccag gatccagccg
     2821 ccccaccagg tggcgcggaa gggcagggcc gccagcgcca gccccacggc caggagcagg
     2881 acgaagagcg ccagccggcg ctgccggggc ggcctatctg gctgtaacat cagcaacaaa
     2941 tactgaaata acgcggcgca taaagtggca cttgacgact tgacgcactg tgttatgctc
     3001 cgtccagatg tggtagcccg gtccaatccc agtgaccggc gggggacccg tggggacctc
     3061 gcgccgtaga cg
//
LOCUS       DASUFA010000190         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1533813, whole genome shotgun sequence.
ACCESSION   DASUFA010000190 DASUFA010000000
VERSION     DASUFA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533813"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFX87_06870"
     CDS             complement(<1..663)
                     /locus_tag="VFX87_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4994671.1"
                     /translation="MDSKDLNWAGPDVRSILGRALDGEELTVAEGIRLTEAEGRDYHA
                     LCLVADEMRRRQAGDVVTYVVNRNINFTNVCIKHCTFCAFSRDHREEEGYFLPLDEVV
                     RRAIEAWEMGATEVCIQAGLPPKLDGSYYVDLCRAIKAAVPEMHLHAFSPEEVLYGST
                     RAGLAIPEYLKLLKSAGLGTLPGTSAEILDQEIRDRIARGRITVDQWVEVITSAHALG
                     IRT"
     gene            complement(690..1877)
                     /gene="cofG"
                     /locus_tag="VFX87_06875"
     CDS             complement(690..1877)
                     /gene="cofG"
                     /locus_tag="VFX87_06875"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX4994672.1"
                     /translation="MSAVDRALARAASGRVLDAAQAEALLATPDERLPELLAPAAARR
                     DRGHGRRLTFSAKVFVPLTTLCRDYCGYCTFRKDPGQPGAHTMTPDEVIALVQAGERL
                     GAKEALFSLGDKPEALFPEHREFLRRMGYRTTLAYLRGVAERVLRESALLPHANPGVM
                     TERDLSALREVNVSMGIMLETTSERLLGPGLAHDRAPDKIPARRLKTIALAGKLQIPF
                     TTGILIGIGETHAERVDALLAIRALHEQYGHIQEVIIQNFRAKPTIPMRGVPDPTLPE
                     LLRTLAVARLLLGGEANIQAPPNLADAGYGGLPAAGLNDWGGISPLTPDHINPERPWP
                     LLRELRRATEAAGHELRERLAIYPEYTTRPEFLDGALRSRVADYVGPDGLVRRDLERW
                     RSW"
     gene            complement(1874..2308)
                     /locus_tag="VFX87_06880"
     CDS             complement(1874..2308)
                     /locus_tag="VFX87_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX4994673.1"
                     /translation="MPSLPEPVAGFISAGRVGRLGTADATGDPLVVPFCYAWDGEALF
                     SAIDAKPKRTAAGELRRVRNIRANPKVSVVIDEYDEDWSRLRWVIIQGRAEILSGGPD
                     YRRGAELLLDKYPQYRRMGLPPEPGTMIKVTPARISHWSFAP"
     gene            complement(2316..>2865)
                     /locus_tag="VFX87_06885"
     CDS             complement(2316..>2865)
                     /locus_tag="VFX87_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994674.1"
                     /translation="GRVTDEFIHLMREMWTREPVDFRGQYYTLGKVSALPKPVQKGGI
                     PVWTGGHTDPALRRAGQVADGWHPIGLRPPGLLEPKEYAEKVAVVREWARKAGRDPKA
                     ITMSYRVPLELRPRGAKPAGGDRTLFRGTVAEVVEDIRTYQRLGVTHFVWDLTATDLK
                     GALALLERFARDVRPKVQRLPR"
BASE COUNT          398 a          952 c         1085 g          430 t
ORIGIN      
        1 ggtgcggatg ccgagcgcgt gggcgctggt gatcacctcc acccactggt cgaccgtgat
       61 gcggccgcgc gcgatgcggt cgcggatctc ctggtcgagg atctccgccg aggtgccggg
      121 gagggtgccg aggcccgccg acttgaggag cttcaggtac tcggggatgg ccaggcccgc
      181 gcgggtggag ccgtagagca cctcctcggg cgagaaggcg tgcagatgca tctcgggcac
      241 cgccgccttg atggcgcggc agagatcgac gtagtaactg ccgtcgagct tggggggcag
      301 cccggcctgg atgcagacct cggtggctcc catctcccag gcctcgatgg cgcggcgcac
      361 gacctcgtcg aggggtagga agtagccttc ctcctcgcga tggtcgcggc tgaaggcgca
      421 gaaggtgcag tgcttgatgc agacattggt gaagttgatg ttgcggttga cgacgtaggt
      481 gacgacgtcg ccggcctgac ggcgccgcat ctcgtcggcg acgaggcaaa gggcgtggta
      541 gtcgcgcccc tcggcctcgg tcagccggat gccctcggcc accgtgagct cctcgccgtc
      601 cagcgcgcgg cccaggatgc tccgcacgtc gggtcccgcc cagttcaggt ccttgctgtc
      661 cacgggtcga tgtcctccgt actcagagac taccagcttc gccagcgctc caggtcccga
      721 cggacaaggc cgtccggacc gacatagtcg gccacgcggg agcggagcgc gccatcgaga
      781 aattcggggc gggtggtgta ctcgggatag atggcgaggc gctcgcgcag ctcgtgtccg
      841 gccgcctcgg tggcccgccg cagctcgcgc agcaaaggcc acggccgctc gggattgatg
      901 tggtccggcg tcagcggcga gatgccgccc cagtcattga ggccggcggc gggcaggccg
      961 ccgtagccgg cgtcggcgag attcggcggc gcctggatat tcgcctcccc cccgagcagg
     1021 agtcgcgcca ccgccagggt gcgcaggagc tcgggcaggg tcgggtcggg caccccgcgc
     1081 atcgggatgg tgggcttggc ccggaagttc tggatgatga cttcctggat atggccgtat
     1141 tgctcgtgca gcgcgcggat ggcgaggagc gcgtccacgc gctcggcatg ggtctcgccg
     1201 atgccgatga ggatcccggt ggtgaagggg atctggagct tgcccgccag cgcgatggtc
     1261 ttgagccggc gcgccgggat cttgtcgggc gcgcggtcgt gggcgagacc ggggccgagc
     1321 agccgctcgg aggtggtctc cagcatgatg cccatgctga cattgacctc gcgcagggcc
     1381 gagaggtcgc gctcggtcat cacgcccgga ttggcgtggg gcaggagggc ggactcgcgc
     1441 agcacgcgct cggcgacgcc gcgcaagtag gcgagcgtcg tccggtagcc catgcgcctc
     1501 aggaactcgc gatgctcggg aaagagcgcc tccggcttgt cgccgaggga gaagagcgcc
     1561 tccttggcgc ccagccgctc gcccgcctgg accagcgcga tcacctcgtc gggcgtcatg
     1621 gtgtgggcgc ccggctggcc cgggtccttc cggaacgtgc agtagccgca gtagtcccgg
     1681 cagagcgtgg tcagcggcac gaacaccttg gccgagaagg tgagccggcg gccatggccc
     1741 cgatcgcggc gcgcggcggc gggggcgagc agctcgggaa gccgctcgtc cggggtggcc
     1801 aggagcgcct ccgcctgggc ggcgtccagg acgcggccgg aggcggcgcg ggcgagagcg
     1861 cgatccaccg cgctcatggg gcgaagctcc agtggctgat ccgggccggc gtcaccttga
     1921 tcatggtccc gggctcgggc ggcaacccca tccgccggta ctgcggatac ttgtcgagga
     1981 ggagctcggc gccgcgccgg tagtccggcc cgccgctgag gatctcggcc cggccctgga
     2041 tgatgaccca gcgcaggcgg ctccagtcct cgtcgtactc gtcgatgacc acgctcacct
     2101 tcgggttggc gcggatgttc cgcacccggc ggagctcgcc ggcggccgtg cgcttgggct
     2161 tggcgtcgat ggcggagaag agcgcctcgc cgtcccaggc atagcagaag ggaacgacga
     2221 gcgggtcgcc ggtggcgtcg gcggtgccga ggcggcccac gcgaccggcg gaaatgaagc
     2281 ccgcgacggg ctcgggcagg gacggcatgg gcccgctagc gcgggaggcg ctggactttg
     2341 ggacgcacgt cgcgggcgaa gcgctcgagg agcgcgagcg cgcccttgag gtccgtggcg
     2401 gtgaggtccc agacgaaatg cgtgaccccg aggcgctgat aggtgcggat atcctccact
     2461 acctccgcca ccgtgccccg gaacagcgtg cggtcgccgc ccgcgggctt ggccccgcgc
     2521 ggccggagct cgagaggcac gcgatagctc atggtgatcg ccttgggatc gcgacccgcc
     2581 ttgcgcgccc actcccgcac caccgcgacc ttctcggcgt actccttggg ctcgagcagt
     2641 ccgggcgggc gcagcccgat gggatgccag ccgtcggcga cctgtccggc gcgtcgcagc
     2701 gcggggtcgg tatgtccgcc cgtccacacc gggatgccgc ccttctggac gggcttgggc
     2761 agcgcgctga ccttgccgag cgtgtagtac tggcctctga agtccaccgg ctctcgcgtc
     2821 cacatctccc gcatgaggtg gatgaactca tcggtgactc gcccg
//
LOCUS       DASUFA010000191         8658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1167911, whole genome shotgun sequence.
ACCESSION   DASUFA010000191 DASUFA010000000
VERSION     DASUFA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167911"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFX87_06890"
     CDS             <1..332
                     /locus_tag="VFX87_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4994675.1"
                     /translation="ALVGGAAKSERTSLNHFAFEVGSLDEVFRARAWLRERSVPIHFE
                     GRRRAGCQIAIEFQDPDGNNLEIYWNIDQVGTEGAVRPAGEWRPAKTLEEAVANPVKG
                     QTLPRRE"
     gene            complement(339..1871)
                     /locus_tag="VFX87_06895"
     CDS             complement(339..1871)
                     /locus_tag="VFX87_06895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4994676.1"
                     /translation="MDIIPFLFFVALFFNIRASIKLTLDWRGMLITTRFVREAYARLA
                     DLPTEERLEREPDAPVFLHLVPAYHEPDIAVTVGALAASRYPHGRLHVVVVTKEEEER
                     SPHPAMGVSTGELVRRLRETLPPYQQKRLSHVVMPGPGRKAHQLNWALRPEVLREILT
                     EETDPRRVFVGVSDADSIPDPDTYRWIAQRELAGQGSLAYQGIPLSLANYDRLTIRGK
                     ICAIQQSSIFIRVSIARLLNEVKRVRLLARLTARAPRLAWLVRPAFELFFRRSQICLG
                     HNQFVRLDLMQAVGGFPTTGATEDSTLGYELGRRGVLIQALPMVEVTDLPETSEGVIR
                     QNARWYRGVLDDIPFLWRVWRMEPTAFNLAQLVRHIGNKVVEWPIAALVYPVTGWLGW
                     YLAYHHEGHRFWFYMATGAPTVSLLLTVWVGGIMTQALIEDLHPYLPRPVDLRRKSLK
                     EKFLGTFRCQTYWLLATRGAWRVLWALARTGRFEPAKTDRITGRRLGTLSPLGRGQGE
                     GS"
     gene            complement(1877..2167)
                     /locus_tag="VFX87_06900"
     CDS             complement(1877..2167)
                     /locus_tag="VFX87_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX4994677.1"
                     /translation="MKGRALILAALGGATLLTLTYGGQSLARVWQMKHEVESLEREIV
                     TLRAETGRLTATVNRLRTDPDFIEQVAREDLGLVKPGERVLKLPPSPGQRGR"
     gene            complement(2164..3450)
                     /gene="eno"
                     /locus_tag="VFX87_06905"
     CDS             complement(2164..3450)
                     /gene="eno"
                     /locus_tag="VFX87_06905"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX4994678.1"
                     /translation="MSEIRATHAREVLDSRGNPTVEVEIWLESGAFGRAIVPSGASTG
                     KREAVELRDDNDNRYGGKGVRRAVQNVNEVIAPEIEGLEAGEQAVLDRALLELDGTPN
                     KSGLGANALLGVSLAAARASADDAGLPLYQYLGGPGARILPVPLMNVLNGGAHADNGL
                     DVQEFMLVPAGAGSFSDALRTGVEIFHTLKRLLKDQGLSTGVGDEGGFAPALGGNEPA
                     LDFLLRAIERTGYRPGEDVWLAVDVAASEFGEHGRYRLRADKTEKSSQEMIAFYEALM
                     ARYPICSIEDGLAEDDWEGWHALTRRIGDRCQLVGDDVFVTNPAILQEGIRKGIANAI
                     LVKVNQIGTLTETLEAIELAKRAGYGTVISHRSGETEDAFIADLAVGVNAGQIKTGSL
                     ARGERTAKYNQLLRIEEELGQAAVWPGRAVFGSRIR"
     gene            complement(3454..4314)
                     /locus_tag="VFX87_06910"
     CDS             complement(3454..4314)
                     /locus_tag="VFX87_06910"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX4994679.1"
                     /translation="MKHRAAAVDAALERFLPAETLRPETLHKAMRYSVFAGGKRLRPV
                     LVIAGAEAVGGTASQVMPAACAMELIHTYSLVHDDLPAMDNDDFRRGAPTNHKVFGEA
                     MAILAGDALLTLAFRLVADNAGQSAALREVVMDIADAAGHKGMVAGQVADLEAEGRRV
                     GADTVDYIHAHKTGALIRTSLRIGAMLCGADAAQVRALSVAGADLGLAFQIVDDILDV
                     VASSEELGKTAGKDQIQQKATYPAIHGIEASRARAAFLIRDAEEALRVLGPRAEPIRA
                     LGRFILERKA"
     gene            complement(4335..4574)
                     /locus_tag="VFX87_06915"
     CDS             complement(4335..4574)
                     /locus_tag="VFX87_06915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX4994680.1"
                     /translation="MTDIKFETALQRLEQIVDQLEAGNLSLEESLQVFEEGVGLARRC
                     GKYLDEAEKRIELLTRDESGVLKVAPFDLAEDDRA"
     gene            complement(4555..5910)
                     /gene="xseA"
                     /locus_tag="VFX87_06920"
     CDS             complement(4555..5910)
                     /gene="xseA"
                     /locus_tag="VFX87_06920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX4994681.1"
                     /translation="MSERVVCTVSELAMRIKASLEDQFPAVWVEGEISNLRTPSSGHA
                     YFTLKDDSAQLRCVLFRGRGRRVAFQPEDGMQVLAFGGLDVYLARGEYQLVVELLEPK
                     GVGALQLAFEQLKRRLEAEGLFDAARKRGLPPYPRTIGIVTSPTGAAIRDMLHVIGRR
                     FADLRILITPVRVQGEEAPGEIVRALADLQEIPDLDVVIVGRGGGSIEDLWAFNDERV
                     ARAIAACRVPVISAVGHETDFTIADFVADLRAPTPSAAAELVVQEKLQVVRALCDLYD
                     TLKQAMALRLARERERVDVLARRRVLTDASRALRDLHRRLDELTTRLGLAARSRRQHA
                     AHRVSLARNALRSLNPVARIANGTTLLAQLRGRLASAAVHSVKASRHRFDAAVGQLDS
                     LSPLAVLGRGYSLTRLSSGVIVRTAAQARPGDAIEILLHQGVLDARVERVKERDDRHQ
                     V"
     gene            complement(6005..6856)
                     /gene="folD"
                     /locus_tag="VFX87_06925"
     CDS             complement(6005..6856)
                     /gene="folD"
                     /locus_tag="VFX87_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX4994682.1"
                     /translation="MLILSGKEVAAKVLAEVKASVAVLRERTGAQPTLAVILVGEDPA
                     SQIYVRNKKRAADEVGIATRDYLFPQGCSQAELLETIAGITRDPAGHGILLQLPLPKG
                     MDEDAAVAAIAPEKDADGLHPMNLGNLLGGKPGAIPCTPAGCVEILDHYGIPIEGAEA
                     VVIGRSRLVGKPLAQLLLGRNATVTMCHTRTRDLAAHCRRADILCVAAGRAGVVTGDM
                     VREGVVVIDVGMNRLPSGKVTGDVHFDSVAPKARAITPVPGGVGPMTIAMLMRNTHRA
                     ALRQLSR"
     gene            complement(6865..7659)
                     /locus_tag="VFX87_06930"
     CDS             complement(6865..7659)
                     /locus_tag="VFX87_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX4994683.1"
                     /translation="MSKPLTVICVGDVFGEPGRRAVQMLLPKLKKQHEADLAVVNVEN
                     SASGFGVTPLIARTFLEQGVDVMTSGNHIWDKKEIIEYIGKENLLLRPANYPPGTPGA
                     GSITVKAGPHKVAVLNLMGRVFLPQLDCPFRKADEELERLRAETPIIVVDMHAEATSE
                     SQAMGWHLDGRVSVVVGTHRHVQTADERLLPKGTAYITDLGLTGPTDSVIGVEPELAL
                     ARFLSGMPNRFEPAKGPAMLQGAVIRIDPETGRGLSIERLRVPLAG"
     gene            complement(7656..>8658)
                     /gene="rny"
                     /locus_tag="VFX87_06935"
     CDS             complement(7656..>8658)
                     /gene="rny"
                     /locus_tag="VFX87_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX4994684.1"
                     /translation="KKAQWLMGLAVQRLASDFVSETVVSVVELPSDEMKGRIIGREGR
                     NIRALEGFTGVDLIVDDTPEAVLISAYDPYRREIARLALQRLIADGRIHPARIEEVVE
                     KVKKDMEQHIREEGDKACFEVGVHGLHPELIKLVGRMKFRTSYGQNCLQHSKEVAWLA
                     GMMAAEIGADVQLAKRMGLLHDIGKALTHEQEGSHPELSLQVLTKYNESPQVINAALC
                     GHENVEAETIEAVLTEAADGISAARPGARRDVLESYIKRLAKLEEIAMSYKGVELCYA
                     IQAGRELRVMTKAEQISDLDAHQLAKDISKRIEAEMQYPGHIKVVVIRETRAVEVAK"
BASE COUNT         1228 a         3056 c         3076 g         1298 t
ORIGIN      
        1 tcgccctcgt ggggggcgcc gcaaagagcg agcgcaccag cctgaaccac ttcgccttcg
       61 aggtcggctc gctcgacgag gtcttccgag ctcgcgcttg gctgcgcgag cggagcgtgc
      121 ctatccactt cgagggccgg cggcgcgccg gctgccagat cgccatcgag ttccaggatc
      181 ccgacggcaa caacctggag atctactgga acatcgacca ggtcggcacc gagggcgccg
      241 tgcgcccggc cggcgagtgg cgcccggcga agacgctgga agaggcggtg gcgaaccccg
      301 tcaagggcca gacgctaccg cgacgggaat aagagcggct aggacccctc accctgccct
      361 ctccccaggg gggagagggt gccgagacgg cggccggtga tgcggtcggt cttggcgggc
      421 tcgaagcgac cggtacgggc cagggcccag agcacgcgcc aggccccgcg ggtcgcgagc
      481 agccagtagg tctggcagcg gaaggtgccg aggaacttct ccttcaggct cttgcggcgc
      541 agatccaccg ggcgcggcag gtacgggtgg agatcctcga tcagcgcctg ggtcatgatg
      601 ccgcccaccc agacggtcag cagcagcgag acggtgggcg ccccggtggc catgtagaac
      661 cagaagcgat ggccctcgtg gtggtaggcc agataccagc cgagccagcc ggtgaccggg
      721 tagaccaggg ccgcgatcgg ccactccacc accttgttgc cgatgtggcg cacgagctga
      781 gccaggttga aggcggtggg ctccatccgc cagacgcgcc agaggaacgg gatgtcgtcg
      841 agcacgcccc ggtaccagcg cgcgttctgc cggatcacgc cctcggaggt ctcggggaga
      901 tcggtcacct ccaccatggg cagcgcctgg atcagcacgc cgcgccggcc gagctcgtag
      961 ccgagcgtgg agtcctcggt ggcgcccgtc gtcggaaagc cgccgaccgc ctgcatcagg
     1021 tccaggcgca cgaactggtt gtggcccagg cagatctggg agcgccggaa gaagagctcg
     1081 aacgcggggc gcaccagcca ggccaggcgc ggcgcgcgcg cggtcaggcg ggccagcagg
     1141 cgcacgcgct tgacctcgtt gagcaggcgc gcgatggaca cgcggatgaa gatggacgac
     1201 tgctggatgg cacagatctt cccgcggatc gtcagccgat cgtagttggc gagcgagagc
     1261 gggatgccct gataggcgag gctcccctgc ccggcgagct cacgctgggc gatccagcga
     1321 tacgtgtccg ggtccgggat cgagtcggcg tcgctcaccc ccacgaagac ccggcgcggg
     1381 tcggtctcct cggtcaggat ctcgcgcagc acctcggggc gcagcgccca gttgagctgg
     1441 tgcgccttgc ggcccgggcc gggcatcacc acgtgcgaga ggcgcttctg ctggtagggc
     1501 ggcaatgtct cccgcaggcg ccgcaccagc tcgccggtgc tcacccccat cgcgggatgc
     1561 ggggaccgct cctcctcctc cttggtgacc accaccacgt gcaggcggcc atgcgggtag
     1621 cgggaggcgg ccagcgcccc caccgtcacc gcgatgtcgg gctcgtggta ggcgggcacc
     1681 aggtgcagga acaccggcgc gtccggctcc cgctcgaggc gctcctcggt gggcaggtcg
     1741 gcgaggcggg cgtaggcctc gcggacgaag cgcgtggtga tgagcatgcc gcgccagtcc
     1801 agcgtcagct tgatcgaggc gcggatgttg aagaagagcg cgacgaagaa caggaacggg
     1861 atgatgtcca cgcgggctag cgccctctct gcccggggga cggcggcagc ttgaggaccc
     1921 gctcgcccgg cttcaccagg cccaggtcct cgcgggccac ctgctcgatg aagtcgggat
     1981 cggtgcggag gcggttcacc gtcgcggtga ggcgcccggt ctcggcgcgc agcgtgacga
     2041 tctcgcgctc gaggctctcg acctcgtgct tcatctgcca cacccgggcc aggctctggc
     2101 ccccataggt gagcgtgagc agcgtggccc cgccgagcgc ggccaggatc agcgcgcggc
     2161 ccttcaccgg atgcggctcc cgaacaccgc ccgccccggc cagaccgcgg cctgccccag
     2221 ctcctcctcg atgcgcagga gctggttgta cttcgcggtg cgctcgccgc gggccagcga
     2281 gccggtcttg atctggcccg cgttcacccc caccgccagg tcggcgatga aggcgtcctc
     2341 ggtctcgccg gagcggtggg agatgacggt gccgtagccc gcgcgcttgg ccagctcgat
     2401 ggcctcgagc gtctcggtga gggtgccgat ctggttgacc ttgacgagga tcgcgttggc
     2461 gatccccttc cggatgccct cctggaggat ggccgggttc gtcacgaaca cgtcgtcgcc
     2521 aaccagctgg cagcggtcgc cgatgcggcg ggtcagcgcg tgccagccct cccagtcgtc
     2581 ctcggcgagg ccgtcctcga tcgagcagat cgggtagcgc gccatcaggg cctcgtagaa
     2641 ggcgatcatc tcctggctcg acttctcggt cttgtcggcg cgcagccggt agcggccgtg
     2701 ctcgccgaac tcgctggccg cgacgtccac cgcgagccac acgtcctcgc cgggccggta
     2761 ccccgtgcgc tcgatggccc gcagcagaaa gtcgagggcc ggctcgttgc cccccagcgc
     2821 gggcgcgaag ccgccctcgt cgccgactcc ggtggagaga ccctggtcct tcagtagccg
     2881 cttcagcgtg tggaagatct ccacgcccgt gcggagcgcg tcgctgaacg agcccgcgcc
     2941 cgcgggcacc agcatgaact cctgcacgtc gaggccgttg tcggcgtggg cgccgccgtt
     3001 gagcacgttc atgagcggca ccggcaggat gcgcgcgccc gggccgccca ggtactggta
     3061 gagcggcagg cccgcgtcgt ccgcggaggc ccgcgcggcc gcgagcgaca cgccgagcag
     3121 cgcattcgcg cccagacccg acttgttggg ggtgccgtcc agctcgagca gcgcgcgatc
     3181 cagcacggcc tgctcgcccg cctcgaggcc ctcgatctcg ggggcgatga cctcgttgac
     3241 gttctgcacc gcgcggcgga cacccttgcc gccgtagcgg ttgtcgttgt cgtcgcgcag
     3301 ctcgaccgcc tcgcgcttgc cggtggaggc cccggacggg acgatggcgc gaccgaaggc
     3361 gcccgactcg agccagatct cgacctcgac ggtgggattg ccccgggagt cgagcacctc
     3421 gcgggcgtgg gtggcgcgga tctcggacat ggctcaggcc ttccgttcga ggatgaagcg
     3481 cccgagggcg cgaatgggct cggcgcgcgg ccccaggacg cggagcgctt cctccgcgtc
     3541 gcggatcagg aacgcggcgc gggcccgaga ggcctcgatg ccgtggatgg cggggtaggt
     3601 cgccttctgc tggatctggt ccttgcccgc cgtcttgccc agctcttcgg agctcgcgac
     3661 cacgtcgagg atgtcgtcca cgatctggaa ggccagcccc aggtcggcgc ccgccacgct
     3721 gagcgcgcgc acctgcgcgg cgtcggcccc gcacagcatg gctccgatgc ggagcgaggt
     3781 gcggatcagc gcccccgtct tgtgcgcgtg gatgtagtcc accgtgtcgg cgcccacccg
     3841 gcggccctcc gcctccaggt cggccacctg acccgccacc atgcccttgt ggccggccgc
     3901 gtcggcgatg tccatgacga cttcccgaag ggcggcgctc tggcccgcgt tgtcggccac
     3961 caggcggaag gcgagcgtca ggagcgcgtc cccggccagg atggccatgg cctcgccgaa
     4021 gaccttgtgg ttggtgggcg caccgcggcg gaagtcgtcg ttgtccatcg cgggcaggtc
     4081 gtcgtggacg agcgagtagg tgtggatcag ctccatcgcg cacgccgcgg gcatcacctg
     4141 cgaagcggtg ccgcccaccg cctccgctcc cgcgatgacc agcaccgggc ggagacgctt
     4201 gccacccgcg aagacgctgt agcgcatcgc cttgtgcagg gtctcggggc ggagggtctc
     4261 ggcgggcagg aagcgctcga gcgccgcgtc caccgcggcc gcccggtgct tcatgtaggc
     4321 gccgagatcg aagctcacgc gcgatcgtcc tcggccagat cgaagggcgc gaccttcagc
     4381 accccgctct cgtcccgggt caggagctcg atgcgcttct ccgcctcgtc gaggtacttg
     4441 ccgcagcggc gggccaggcc caccccctcc tcgaacacct gcagcgactc ctcgagggag
     4501 agattgccgg cctcgagctg gtccacgatc tgctccagcc gttgcagcgc cgtttcaaac
     4561 ttgatgtctg tcatctcgtt ccttgacgcg ttccacccgg gcgtccagca cgccctggtg
     4621 cagcaggatc tcgatggcgt cgcccgggcg cgcctgcgcg gcggtgcgaa cgatcacgcc
     4681 cgaagacagc cgcgtcaggc tgtagcctcg cccgagcacg gccagcgggg agaggctgtc
     4741 gagctgcccg accgcggcgt cgaaccggtg acgggacgcc ttcacgctgt gcaccgccgc
     4801 cgaggcgagc cggcctcgca gctgtgcgag caacgtcgtg ccgttggcaa tcctagcgac
     4861 cgggttgaga gagcgcaagg cgtttctggc cagagacacc cgatgtgcgg cgtgctgccg
     4921 ccggctccgc gccgcgaggc cgagccgggt ggtcagctcg tcgaggcgcc ggtgcagatc
     4981 gcgcagcgcg cgtgaggcgt cggtgagcac gcggcgccgg gcgaggacgt ccacgcgttc
     5041 ccgctcgcgc gcgaggcgca gcgccatcgc ctgcttgagc gtgtcgtaca ggtcgcacag
     5101 cgcccggacc acctgcagct tctcctgcac caccagctcg gcggccgccg acggcgtggg
     5161 cgcccgcagg tcggccacga agtccgcgat cgtgaagtcg gtctcgtgac ccaccgccga
     5221 gatcaccggc acgcggcacg cggcgatcgc gcgcgccacc cgctcgtcgt tgaaggccca
     5281 gagatcctcg atggagccgc cgccccggcc cacgatcacc acgtccagat ccgggatctc
     5341 ctggagatcg gcgagcgcgc gcacgatctc gcccggggcc tcctcgccct gcacgcgcac
     5401 cggcgtgatc aggatgcgca ggtccgcgaa gcggcgcccg atgacgtgga gcatgtcgcg
     5461 gatggccgcg ccggtgggcg aggtcacgat cccgatggtg cgcgggtacg gcggcagccc
     5521 gcgcttgcgc gcggcgtcga agaggccctc ggcctcgagc cggcgcttga gctgctcgaa
     5581 ggcgagctgg agcgcgccca cgcccttggg ctcgagcagc tccaccacca actggtattc
     5641 cccgcgcgcg aggtacacgt cgaggccgcc gaaggccagc acttgcatgc cgtcctcggg
     5701 ctggaaggcc acgcggcggc cgcgaccgcg gaagagcacg cagcggagct gggcgctatc
     5761 atccttcagc gtgaagtagg cgtgccccga cgagggcgtg cgcaggttcg agatctcccc
     5821 ctccacccac accgccggga actgatcctc gagcgacgcc ttgatccgca tcgccagctc
     5881 actcacggtg cagacgaccc gctcgctcat cgggaccccc tcaccctacc ctctccccag
     5941 aggggagagg gtatgtggtg tgccccccgg aggggagagg gtctgtaggc cgccgatgtc
     6001 tcgctcatcg ggagagctgg cggagggcgg cgcggtgggt gttgcgcatg agcatcgcga
     6061 tggtcatggg gccgacgcca ccgggcaccg gggtgatggc gcgggccttg ggggccaccg
     6121 agtcgaagtg cacgtcgccg gtgaccttgc cgctgggcag gcggttcatg cccacgtcga
     6181 tcacgaccac gccctcgcgc accatgtcgc cggtgaccac gcccgcgcgg cccgccgcga
     6241 cgcagaggat gtcggcgcgc cggcagtgcg cggccaggtc ccgcgtgcgc gtgtggcaca
     6301 tggtgacggt ggcgttgcgc ccgagcagga gctgcgccag gggcttgccg acgaggcgcg
     6361 agcgcccgat caccaccgcc tccgcgccct cgatcgggat gccgtagtgg tcgaggatct
     6421 cgacgcagcc ggccggagtg cacgggatcg ccccgggctt gccgcccagc aggttgccca
     6481 ggttcatcgg gtgcagcccg tccgcgtcct tctcgggggc gatcgccgcc accgccgcgt
     6541 cctcgtccat gcccttgggc agcgggagct ggagcaggat gccgtggccg gccggatcgc
     6601 gggtgatgcc cgcgatggtc tcgagcagct cggcctgcga gcagccctgg gggaacaggt
     6661 agtcgcgcgt ggcgatgccg acctcgtcgg ccgcgcgctt cttgttgcgg acgtagatct
     6721 gggaggcggg gtcctcgccg accaggatga ccgccagcgt gggttgcgcg cccgtccgct
     6781 cccgcagcac ggcgacgctg gccttcacct cggccaggac cttcgccgcg acctctttgc
     6841 cgctgagaat cagcaccggc ccactcagcc ggcgagaggg acgcgcagac gctcgatgga
     6901 cagcccgcga ccggtttcgg gatcgatgcg gatcaccgcg ccctggagca tggccggccc
     6961 cttggcgggc tcgaagcggt tcggcatccc cgagaggaag cgggcgaggg ccagctcggg
     7021 ctcgacgccg atgaccgagt ccgtcgggcc ggtgagcccc aggtccgtga tgtaggcggt
     7081 gcccttgggc aggagccgct cgtccgcggt ctgcacgtgg cggtgcgtgc cgaccaccac
     7141 gctcacccgg ccgtcgaggt gccagcccat ggcctgcgac tcggacgtcg cctcggcgtg
     7201 catgtccacc acgatgatcg gcgtctcggc gcgcaggcgc tccagctcct cgtcggcctt
     7261 gcggaagggg caatcgagct gcggcaggaa gacgcggccc atcaggttga ggaccgcgac
     7321 cttgtgcggc ccggccttga ccgtgatgga gcccgcgccg ggagtacccg gcgggtagtt
     7381 ggccgggcgc aggagcaggt tctccttgcc gatgtactcg atgatctcct tcttgtccca
     7441 gatgtggttg cccgaggtca tcacgtccac gccctgctcg aggaaggtcc gcgcgatcag
     7501 cggggtgacc ccgaagcccg aggccgagtt ctccacgttg accaccgcga gatcggcctc
     7561 gtgctgcttc ttgagcttgg gcaggagcat ctggaccgcg cggcgccccg gctccccgaa
     7621 cacgtcgccc acgcagatga cggtgagcgg cttgctcact tggccacctc gaccgcgcgg
     7681 gtctcgcgga tcaccacgac cttgatgtgg ccggggtact gcatctcggc ctcgatgcgc
     7741 ttggagatgt ccttggcgag ctggtgggcg tcgagatcgg agatctgctc ggccttggtc
     7801 atgacccgca gctcgcggcc ggcctggatc gcgtagcaga gctccacgcc cttgtaggac
     7861 atggcgattt cttccagctt cgccaagcgc ttgatgtagc tctcgaggac gtcgcgccgg
     7921 gcgcccggcc gcgcggcgga tatgccgtcg gcggcctcgg tgagcaccgc ctcgatggtc
     7981 tccgcctcga cgttctcgtg cccgcagagc gcggcgttga tcacctgggg cgactcgttg
     8041 tacttggtga gcacctgcag cgacagctcg gggtggctcc cctcctgctc gtgggtgagc
     8101 gccttgccga tgtcgtggag caggcccatc cgcttggcga gctgcacgtc ggcgccgatc
     8161 tcggccgcca tcatgccggc cagccacgcc acttccttcg agtgctgcag gcagttctgc
     8221 ccgtaggagg tgcggaactt catgcgaccc accagcttga tcagctcggg atgcagcccg
     8281 tgcacgccga cctcgaagca ggccttgtcg ccttcctcgc ggatgtgctg ctccatgtcc
     8341 ttcttgacct tctccaccac ctcctcgatg cgggcggggt gaatgcggcc gtcggcgatc
     8401 agccgctgga gcgcgagccg cgcgatctcc cgacggtagg gatcgtaggc cgagatcagc
     8461 accgcctccg gggtgtcgtc cacgatcagg tccaccccgg tgaagccctc gagagcccgg
     8521 atgttgcgcc cctcgcgacc gatgatccgc cccttcatct cgtcgctcgg cagctcgacg
     8581 accgagacca cggtctcgga gacgaaatcc gaggcgagcc gctggacggc cagccccatc
     8641 agccactgcg ccttcttc
//
LOCUS       DASUFA010000192         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_961614, whole genome shotgun sequence.
ACCESSION   DASUFA010000192 DASUFA010000000
VERSION     DASUFA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961614"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1492
                     /locus_tag="VFX87_06940"
     CDS             128..1492
                     /locus_tag="VFX87_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HEX4994685.1"
                     /translation="MLEEPTSLRIDDPGVRALFTEPARFQSWLDVEAALARAQAELGV
                     IPEAAAREIVRKAHLSYLDLAAVREGLARTGHPLVPLVWELDRACEGDAGGYVHWGAT
                     TQNVTQTGQLLQVRRAHEIFLRQLAAILTTLADLAERTKDVLLPGRTHGQHAVPATFG
                     FKVAVWIDELGRHVARLQGCEDRVFVAMLGGGAGTLASLGELGLATQERMAAHLGMRP
                     MPLPARTIGDHQAEYVLLLGMLAATSSKIGREIYTLMKQEFGEVEEPVPPGTVGSSTM
                     PQKRNPKLSQDIIAAAAQIRALAPLALEAMQTEHEADRTTSIMMSRAIVQACELTGDM
                     LQRMMTLFDGLQVFPDRMRENLDLSGGLIMAEALMLELGKQIGRQRAHDAVYEAAQGS
                     VTEGRPFREMLALDPHVSAGLTPAQMEALLDPARYAGLCREFAERGAASARAIASAIE
                     LRLA"
     gene            complement(1497..1898)
                     /locus_tag="VFX87_06945"
     CDS             complement(1497..1898)
                     /locus_tag="VFX87_06945"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4994686.1"
                     /translation="MKRQNIQPESLSKRVVGGHVLYSHVVVVEGKRTIFVSGQLARDP
                     KGDVVGKGDMGAQLRQVGENIKAALEAAGATLADIVKTNTYVTDIDEFFKHVDVRMEY
                     FGALPTSTTVEVRKLAHPDLLVEVEAIAVVD"
     gene            1907..2836
                     /locus_tag="VFX87_06950"
     CDS             1907..2836
                     /locus_tag="VFX87_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4994687.1"
                     /translation="MIADREGEDHMLPTHGRYDYSSITARPDYSWPGNRRLAVYIALN
                     IEAFGFGVGKGAAIAPPDQAQSHSVYSWRDYGNRVGIWRLLELLDELGLPAEAQLNAA
                     IYDVAPDIPARLRARGDEILGHGLTNSDEQGHLPEDQERTLIEQATATIARHEGARPV
                     GWMSPWLSNSAVTMDLLQEAGYRYVMDWTMDDQPVWLRTRKGRILAVPYPIEVNDTRG
                     IVWYRYTADEFADMIVDQFDEMLQQSAGQPLVCPISLHPFVIGRPYRIRRLRRALQHI
                     LGHGDRVWLTRPRDICAHVESLPAGVVPGAGVP"
BASE COUNT          435 a         1092 c         1018 g          418 t
ORIGIN      
        1 ccgccgcgcc ctggccccgc gccccgccgc tgatcagcgc caccttccca tcgagcttgc
       61 ccatggccag aatgtaagac ctgaccccac cgttgacaag gcgtgggcgg cgtgcgagag
      121 aatcgccatg ctcgaggagc cgacctcact ccggatcgac gatcccggcg ttcgcgcgct
      181 gttcaccgag cccgcccgct ttcaatcctg gctggacgtc gaggccgcgc tcgcgcgggc
      241 ccaggccgag ctgggcgtca tcccggaggc cgccgcccgc gagatcgtcc gcaaggcgca
      301 cctgtcgtat ctggacctcg cggcggtgcg cgaggggctg gcccggaccg gccatccgct
      361 ggtgccgctg gtgtgggagc tcgatcgcgc ctgcgagggc gacgcgggcg gctacgtgca
      421 ctggggtgcc accacccaga acgtcaccca gaccggccag ctcctccagg tgcgccgggc
      481 ccacgagatc ttcctgcggc agctcgcggc catcctgacc acgctggccg acctggccga
      541 gcgcaccaag gacgtgctgc tgcccggtcg cacccacggc cagcacgcgg tgccggccac
      601 cttcggcttc aaggtcgcgg tgtggatcga cgagctcggc cgccacgtcg cgcggctgca
      661 gggctgcgag gaccgcgtgt tcgtggccat gctgggcggc ggcgcgggca cgctcgcctc
      721 gctgggcgag ctgggtctcg ccacccagga gcggatggcc gcgcacctcg gcatgcggcc
      781 gatgcccctg cccgcgcgca ccatcggcga ccaccaggcc gagtacgtgc tgctgctggg
      841 catgctcgcg gccacctcca gcaagatcgg gcgcgagatc tacacgctca tgaagcagga
      901 gttcggcgag gtggaggagc cggtgccgcc cggcacggtg ggcagcagca cgatgccgca
      961 gaagcgcaat cccaagctct cgcaggacat cattgccgcg gcggctcaga tccgcgcgct
     1021 cgccccgctg gcgctcgagg ccatgcagac cgagcacgag gccgaccgca ccaccagcat
     1081 catgatgagc cgggcgatcg tgcaggcctg cgagctgacc ggcgacatgc tgcagcggat
     1141 gatgacgctc ttcgacgggc tccaggtctt cccggaccgg atgcgcgaga acctcgacct
     1201 ctcgggcggc ctcatcatgg ccgaggcgct catgctcgag ctgggcaagc agatcggccg
     1261 gcagcgcgcg cacgacgcgg tgtacgaggc cgcgcagggc tcggtgaccg agggccgccc
     1321 gttccgcgag atgctggcgc tcgatccgca cgtgagcgcg gggctcaccc cggcccagat
     1381 ggaggccctg ctcgatcccg cgcgctacgc gggactctgc cgggagttcg ccgagcgcgg
     1441 cgccgcctcc gcccgcgcga tcgcctccgc gatcgagttg cggctggcct gaggcctcag
     1501 tccaccaccg cgatcgcctc gacctcgacg agcaggtcgg ggtgggcgag ctttcgcacc
     1561 tccaccgtgg tgctggtggg cagcgcgccg aagtactcca tgcggacgtc cacgtgcttg
     1621 aagaactcgt cgatgtcggt gacgtaggta ttggtcttga cgatgtcggc cagggtggcc
     1681 ccggcggcct ccagcgccgc cttgatgttc tcgcccacct ggcgcagctg cgcgcccatg
     1741 tcgcccttgc cgaccacgtc gcccttcgga tctcgcgcga gctggcccga cacgaagatc
     1801 gtccgcttgc cctcgaccac caccacgtgg gagtagagca cgtggccccc gacgacacgc
     1861 ttgctgagag actcgggctg gatgttctga cgcttcatga ggacgtatga tagcggaccg
     1921 ggagggggaa gaccacatgc tgccgaccca cgggcgctac gactactcga gcatcacggc
     1981 gcggccggac tatagctggc cgggcaaccg gcgtctggcc gtctacatcg cgctcaacat
     2041 cgaggccttc ggcttcgggg tcggcaaggg cgcggccatc gcgccgccgg accaggcgca
     2101 gagccattcc gtctacagct ggcgcgacta cggcaaccgg gtcggcatct ggcgcctcct
     2161 cgagctgctc gacgagctcg ggctgcccgc ggaggcccag ctcaacgccg cgatctacga
     2221 cgtggcaccc gacatccccg cgcgcctgcg cgcccgcggc gacgagatcc tgggccacgg
     2281 gctgaccaac tcggacgagc aggggcatct cccggaggac caggagcgca cgctgataga
     2341 gcaggccacc gccacgatcg cgcgtcacga gggggcccgc ccggtcggct ggatgagccc
     2401 gtggctctcc aacagcgcgg tcaccatgga cctgctgcag gaggcgggct accgctacgt
     2461 gatggactgg accatggacg atcagccggt ctggctccgc acccgcaagg gccggatcct
     2521 cgcggtgccc tacccgatcg aggtcaacga cacacgcggg atcgtctggt accgctacac
     2581 cgccgacgag ttcgcggaca tgatcgtgga ccagttcgac gagatgctgc agcagtcggc
     2641 gggccagccg ctggtgtgcc cgatctcgct gcaccccttc gtcatcggcc ggccgtatcg
     2701 catccggcgg ctgcgccggg cgctgcagca catcctcggc cacggcgacc gcgtctggct
     2761 gacgcgcccg cgcgacatct gcgcgcacgt cgagtcgctg ccggccgggg tcgtgcccgg
     2821 agcgggcgtc ccgtgaagcc cgcgggcatc tcccgccgcg cgctgctcgg aaccggcgcg
     2881 gccgctgcgc tcatgcgggc gttccccgcg gtcgcccagt cgcccccgaa gcctctggtc
     2941 ctcgacgacg ccagccggct caa
//
LOCUS       DASUFA010000193        14623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1534468, whole genome shotgun sequence.
ACCESSION   DASUFA010000193 DASUFA010000000
VERSION     DASUFA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534468"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1184
                     /locus_tag="VFX87_06955"
     CDS             189..1184
                     /locus_tag="VFX87_06955"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4994688.1"
                     /translation="MRVDQPSVQIAPEDVELKKSWTRESVSPEGWLISLPPDCVAELD
                     EVAARVRATPRPVDTLDPADFSLRACTAVMASVRAKLETDTGLAVVDRFPVERYRHPE
                     NLAIGWLLAAMLGQVVAQKADGTRLYDVKDSGQALGYGVRRSVTNLGQPFHTDGGWLW
                     KAPAFVGLFCLESAMEGGLSRFVSLVTAHHELRRRRPDLLARLYRPFHWDRQGEHAPD
                     ASRVSRQPVFEFEGGTLGSRYYEDYIRNGSKMAGEVLDDEGAQALEAMREIVDDPANW
                     VEFRIEKGQLQYLNNRQFAHSRTAFKDAGPGQGRHMLRLWNRDEGTWHLEGSPSA"
     gene            1200..1487
                     /locus_tag="VFX87_06960"
     CDS             1200..1487
                     /locus_tag="VFX87_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994689.1"
                     /translation="MILEPAVYSGLVVVLAYWVRGMAGFGSGLIAVPLLSLMWPVTLV
                     VPIVVALDYLGSASQGIKSADQVAWGERPRATAVSWAAWSARCSGRAGPST"
     gene            1403..1807
                     /locus_tag="VFX87_06965"
     CDS             1403..1807
                     /locus_tag="VFX87_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX4994690.1"
                     /translation="MGGAAASYCGLLGGLVGTLFGTGGPFYVIYLNLRGLDRSVFRAT
                     FAMNFLIDGGVRLAAFVAAGLYHRQPLGYLLAAVPIAAAGLYVGGRIQTGLSQRTFHL
                     VIRVLLLTSGLALLLKRSPASGLGDRAWRSLP"
     gene            complement(1811..2983)
                     /locus_tag="VFX87_06970"
     CDS             complement(1811..2983)
                     /locus_tag="VFX87_06970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994691.1"
                     /translation="MNVRPDPNLVLITAGVTGIVTSVTMIGPLLVDISRDLGISVSQA
                     GLLAVAAGVPQALFSPFSGLLSDRLGRRPMIVTAFASVGVLSFTAALTPGFAALVAIR
                     FLAGLLGSLAPSSLMASVGDLFPAERRARAMGWFNMGFSFAAIGGVPVMGALGGGFGW
                     RWAFAAIGLVMLLLAGCVWRWFPRIPPMASGTSVVATYQAVWGVPRLLNLLGANLMER
                     SLFGMVAIYLAAFVMLSFHLSAVGVAPVLVLVAVGAIAGNVLGGRLGDRFNKPGIFVI
                     AQIISAGLGLALFGAGLPFAAVVGLAMLLTLCNSASRPAFLAYGSELAPRQRGALFGL
                     IALTNQSGLVIGSAVGAAAIGGAGYQGMALAALAQGVLAAGLALPLLRPPRALGDP"
     gene            complement(2980..3663)
                     /locus_tag="VFX87_06975"
     CDS             complement(2980..3663)
                     /locus_tag="VFX87_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994692.1"
                     /translation="MGFDFAFGYPADAGLPAGRALCARLDGLIRDAPDGANNRFEVAG
                     ILNREIQAAFGGGFDGPFWGHPAGHEYPHLFRTRPRPFPWRIRDGRLVERRLASRHIQ
                     SPWKLFTRGAVGSQTLLGLPAVHRLLTDGALAARTRLWPFETAWDAAVGDDSIVIAEL
                     WPSLVDCSRQPYPVKDACQVAAVRDWALDAPDSLARSLARPPDLTAEDERIAREREGW
                     IVPNVYPNV"
     gene            complement(3858..4886)
                     /locus_tag="VFX87_06980"
     CDS             complement(3858..4886)
                     /locus_tag="VFX87_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4994693.1"
                     /translation="MKALAFHEHGGLDKLRYQDVPDPTIGAGDVLVRVRAAALNHLDL
                     FVREGLPGLTLPLPFWTGCDIAGDVAQVGAAVRDVTVGDRVAVNPNLSCGRCEYCRRG
                     EHSMCVRYGILGEHTHGGLAEYVKVEAHHVLPLPGAIRYEDAAAFILVNMTAWRMLVT
                     QARLRAGEDLLVIGVGGGVSSTAVQIGKLCGARVWVTSSSDDKLERARALGADECINY
                     AKEDWVKVIGQKTGRRGVDVVLENVGAATWKGAIRAVAKGGRIVTCGATSGPIAETDI
                     RLVFWKQLSIIGSTMSNDAEFNTVMGQLFAGRLRAIVDTVMPLKDGVEAQRRLAEGRQ
                     FGKIVLTP"
     gene            complement(4883..6121)
                     /gene="ccrA"
                     /locus_tag="VFX87_06985"
     CDS             complement(4883..6121)
                     /gene="ccrA"
                     /locus_tag="VFX87_06985"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX4994694.1"
                     /translation="MADLYDLGDKPPLGEVPKRMHAFAVRQNRFGQPREAWKREVLPT
                     PTIGPDEVLIYVMASGINYNNVWAALGQPLDVIAERLKMGEPEDFHAGGSDCSGIVWA
                     TGHDVKNVKVGDEVIVHSGWWRPDDPWVLSGKDPMLAESTRIWGYQTNYGSYCQFARA
                     QAHQCLAKPKRLTWEASGCFLLCASTAYRMLMGWEPHTVEKGDVVLVWGATGGLGSLA
                     LEIVRAQGGRAIAVVSDDAKKKFCMDHGAVGVINRSQFDHWGPMPDTKDGKAYGNWAK
                     GARAFGKAIWDALGERTSPRIVFEHPGEATLPTSEFVCGTGGMIVICAGTTGYNVTLD
                     LRYHWMRQKRFQGSHLSNDQQALAVTRLVDAGKVDPCLSQTYGFDELPACHQLMLDNK
                     HPYGNMAVLVNAPKAGLGAS"
     gene            complement(6136..6945)
                     /locus_tag="VFX87_06990"
     CDS             complement(6136..6945)
                     /locus_tag="VFX87_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4994695.1"
                     /translation="MTDKVQVEIKGDIATIMLNKPERLNALDNEMWHGIGEACRVVDA
                     APDVRVAVLMGAGGCFCAGLDVKAGSTLSTALEDMSPGQALARIRRHLEGHQASFSAV
                     EALRVPVIGAIQRACMGAGLELALCCDVRLAAEGTLFSIPEVQLGLVPDMGGTQRLPR
                     TVGIGKAKELIYSARRFDAAEAHRIGLVQAVYPAGELGARVAELAGEIAANAPLAVQA
                     AKRAINGTYYGDIGAWLDWETAQASGPLLSEDRKAGLKAAAERKRANFKGR"
     gene            complement(6955..7971)
                     /locus_tag="VFX87_06995"
     CDS             complement(6955..7971)
                     /locus_tag="VFX87_06995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4994696.1"
                     /translation="MKLDVSMLTHDLKTIPDYARKVEAMGYDCLWSSETQHDPYLPVA
                     VAATCTSRIKLGTNIATVFSRSPMITAHIAWDLQKASAGRFTLGLGTQVKAHNERRFS
                     VKFESPGPKMAEAIRAIRTIWDCWQTGGRLNFKGQFYTFDVMTPFFNPGPIDHPKIPI
                     YIAAVNPLMCRIAGELCDGMHVHPFNSPKYLREIVHPAVEEGLKRSGRSRRDFTYATA
                     TFVVVGDTEKERAAQAQTVKQQIAFYGSTRTYQPVLAAHGWEDVTGKLHRKSVEGDWK
                     AMADLITDEMLDTYAVTATYENLHAKILERYQGLLDRTGLYQPYQPNLNDPRLPAMVR
                     AFNG"
     gene            8256..8924
                     /locus_tag="VFX87_07000"
     CDS             8256..8924
                     /locus_tag="VFX87_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994697.1"
                     /translation="MAQKKGTGLLMVWADVPADKEDEFNRWYNEEHLPERMAIPGFLS
                     GARYEAVKGGPKHLAYYELESAAVLETPAYKKVQANPTEWTKRSGPGTIATTFIRNVY
                     TMIHPTALTPEIGGAPMAPVLQIGRMDVPPAADREFNDWYNTVYVPNYEKVPGVIRGR
                     RYRAVEGSPTYLTLYEFEHPKVSESPAWMAQRDASPVNAQMRKHMKHAPGSPGVYVKT
                     VELR"
     gene            9082..10665
                     /locus_tag="VFX87_07005"
     CDS             9082..10665
                     /locus_tag="VFX87_07005"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006077483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX4994698.1"
                     /translation="MYDYVIVGAGSAGCVLANRLSADPATRVLLLEAGGPDTSPFIHT
                     PAMMGFLPDGPHDWRYRTVPQVHCNLRRFAWPRGRTLGGSSSINYMIYIRGHAADYDH
                     WARLGNKGWSYEDVLPFFRKSEHNERLEDRFHGRGGPLNVADHRFRHPLSEMFVAAAE
                     ETGVPPNADFNGAVQDGGGFYQLTQKDGRRWSTASAFLRPALERPNLTVITGALTCRI
                     RIEGRRAVGVEYVHHGQSARADALREVVLCGGSVNSPQLLMLSGIGPAEHLRAVGVPV
                     VADLPGVGRNLQDHLGTFLRNEITVPFSLYGATPEQLAAVQTEYETTGQGLFSSNIAE
                     AGAFLRTDGTDGPPNLQGFFLPYCITEAPLEAFQPYCHGITFAFYVNRPATRGQITLA
                     SADPLDQPLIDPAYLSAPSDLPQFVAGIRMLREIFRAKPLGDILGRELGPGSEARTDA
                     EIAAYVRDRASGTIFHPVGTCKMGTDEMAVVDPALRVHGLDGLRVVDASIMPTLIGGN
                     TNAPTIMIAEKAAAMMVGG"
     gene            10674..11594
                     /locus_tag="VFX87_07010"
     CDS             10674..11594
                     /locus_tag="VFX87_07010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX4994699.1"
                     /translation="MQRVTTVSAGQAPRRLDVFVATQTAHLSRAAAQRWIDGGLITVN
                     GRQTKPARLIRPGDVITCHVPVREPPPVEPEPLALRVLHEDGEILVIDKPPGLVMHPG
                     PGHWTGTLLNALVYHVGDERGAGRRARPGLVHRLDKGTSGVLVIAKTDAAHRDLSRQF
                     RAHSVHRVYLALVSGAVRRGGLIDQRLGRDTRDRRRVSTRSPATRRAVTEYRVAERLG
                     PDATLVEARPHTGRTHQIRVHLASIGHPVLGDASYGTAPADPALPRAMLHAAVLGFAH
                     PASGEYVEFCAPLPADVEHAVARCRAGASR"
     gene            11838..13223
                     /locus_tag="VFX87_07015"
     CDS             11838..13223
                     /locus_tag="VFX87_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX4994700.1"
                     /translation="MDTHPSDVPIIWQPTPARIAELDRLGDEIAELSVHLEAATARLL
                     ALIREFDARGGWANGFRSCAEWLAWRVGLAPGAAREHVRVARALGTLPAMAEGLARGE
                     LSYAKVRALTRIATPETEARLLAVGRAGTAVHVESIVRGWRQVDRQTEARRAARQHAG
                     RALHVYQDEDGTVVLRGRLTPEAGALLLRALDAARETLYQRRRAGQAATPAADPASET
                     PTRPQQQADALALLAETALHHELDPGAPGERYQVVVHVDAAVLANAAQPGQSVLEDGP
                     HVPAGTSQRLACDASRVVMRHDEAGRVVEVGARTRTIPAALRRALQHRDKTCRFPGCT
                     VRIAEGHHVRHWAHGGPTKLSNLALLCRRHHRAVHEEGYQAERGADGTLRFRRPDGRP
                     LPNVPAPPVVAADPAGAIRAQNAAQGLTLHARTGLAQWLGERLDVRWAIDVLHPRAAR
                     IVRRESPSQPG"
     gene            complement(13276..13653)
                     /locus_tag="VFX87_07020"
     CDS             complement(13276..13653)
                     /locus_tag="VFX87_07020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX4994701.1"
                     /translation="MTDIATLDRHRYLSLATFRRSGAEVRTPVWFAAVDGKLYVFTAG
                     ESGKVKRLRYSSRARVARSDMRGHVRGAWYDAAARIVTEPAVIERARAALQAEYGWQM
                     WIGDLFSRLTGRIRRRAWIEIEV"
     gene            complement(13666..>14623)
                     /locus_tag="VFX87_07025"
     CDS             complement(13666..>14623)
                     /locus_tag="VFX87_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994702.1"
                     /translation="WPGLLVPTDLGGSGGSIGDALALVEEMGRACFPSPFIASAVVAT
                     TALTAGGGGRARALLPALALGERTCALAVLEESGRLDPHVMTLPVATPGRLTGRKLFV
                     SDAHVATDVIVLGRAAAGPTALLLPMGRAGITARPLDSMTGDKLFELDLADVEAAPDD
                     LLGAPGGGWMVLAPAFRTGALARSAEIVGAAQRVLELCVEHARVRVQGGRPIGGYQAI
                     QHACADLFRDVESARWLMWEAAWRLESGRPDADPAVAAAKAYCAEAGLRVARRGHQIM
                     GAIGYSEEHPLHLFHKRILAAGVDAGDAALHLEAVARSIGLA"
BASE COUNT         2120 a         5256 c         5175 g         2072 t
ORIGIN      
        1 cgtgcggacg agatcctggg cgcgctgcac ggccgcgcgg tgcccaccat ccgcccccgc
       61 gtgctgcagg tggacggcaa gcccgtaccg gtccttcccg gcgaccctcg ctggtactag
      121 gccgctggtc ctcgcccggc ggggcctgct atactcgcgc tccattgccg ctcgaggagg
      181 ccgttccgat gcgagtcgat cagccgtcgg tccagatcgc ccccgaggac gtcgagctca
      241 agaagtcctg gacgcgtgag agcgtctcgc cggaaggatg gctgatctcg ctcccccccg
      301 actgtgtggc cgagctcgac gaggtggcgg cccgggtgcg ggccacgccc cgcccggtgg
      361 acaccctcga ccccgcggac ttctccctcc gcgcctgcac cgcggtcatg gcctccgtgc
      421 gggccaagct cgagaccgat accggcctcg cggtggtgga tcggttcccg gtcgagcgct
      481 accgccaccc cgagaacctg gccatcggct ggctgctggc cgcgatgctc gggcaggtgg
      541 tcgcgcagaa ggccgacggc acccgtctct acgacgtcaa ggactccggg caggcgctgg
      601 gctacggggt gcgccgctcc gtgaccaatc tgggccagcc gttccacacc gacggcggct
      661 ggctctggaa ggcgcccgcc ttcgtcggcc tcttctgcct cgagtcggcg atggagggcg
      721 ggctgagccg cttcgtcagc ctggtcaccg cccaccacga gctgcgccga cgccggcccg
      781 atctgctcgc gcggctctat cggccgtttc actgggaccg ccagggcgag cacgcgccgg
      841 acgcctcgcg ggtgagccgc cagcccgtct tcgagttcga gggcggcacg ctgggatcgc
      901 gctactacga ggactacatc cgcaacggct cgaagatggc gggcgaggtg ctcgacgacg
      961 agggcgccca ggcgctcgag gccatgcggg agatcgtgga cgatcccgcc aactgggtcg
     1021 agttccgcat cgagaagggc cagctccagt acctgaacaa ccgccagttc gcgcacagcc
     1081 ggaccgcctt caaggacgcg ggcccggggc agggacgtca catgctccgc ctctggaacc
     1141 gcgacgaggg cacctggcat ctcgagggca gcccttccgc ctgaccgtcg tgccgtccgt
     1201 tgatcctcga gcccgcggtc tactccgggc tggtggtggt gctggcctac tgggttcggg
     1261 gtatggccgg cttcggctcc gggctcatcg cggtgcccct gctgtcgctg atgtggccgg
     1321 tcaccctcgt cgtgccgatc gtggtggcgc tcgactatct cggctcggcc agccagggca
     1381 tcaagagcgc ggaccaggtg gcgtgggggg agcggccgcg agctactgcg gtctcctggg
     1441 cggcctggtc ggcacgctgt tcgggacggg cgggcccttc tacgtgatct atctcaacct
     1501 gcgcgggctc gaccggagcg tgttccgagc caccttcgcg atgaacttcc tgatcgacgg
     1561 cggcgtgcgc ctggccgcgt tcgtggccgc cggcctctac catcgccagc cgcttggcta
     1621 cctgctggcc gcggttccca tcgcggcggc cgggctctac gtcggcggac gcatccagac
     1681 cgggctctcg cagcgcacgt tccacctcgt gatccgcgtc ctgctcctga ccagcggctt
     1741 ggccttgctc ctcaagcgat cgcctgcctc cggattggga gaccgagcct ggcggagcct
     1801 cccctgaggg tcaaggatcg ccgagagcgc ggggcggcct gagcagcggc agcgccagcc
     1861 cggccgcgag cacgccctgg gccagcgcgg ccagcgccat gccctgatag ccggcgcccc
     1921 cgatggccgc agcgcccacc gcggagccga tcacgagccc gctctggttg gtgagcgcga
     1981 tgagcccgaa gagcgcgccg cgctggcggg gcgccagctc ggatccatag gcgaggaacg
     2041 cggggcggct cgccgagttg cagagggtca gcagcatcgc gagccccacg actgccgcga
     2101 agggcagccc cgcgccgaag agagccaggc cgagcccggc gctgatgatc tgggcgatca
     2161 cgaagatccc gggcttgttg aagcggtccc cgagccgacc ccccagcacg ttgcccgcga
     2221 tcgcgccgac cgcgaccagg accagcaccg gcgccacgcc gaccgcgctc aggtgaaagc
     2281 tcagcatgac gaacgcggca aggtagatcg ccaccatccc gaagagcgag cgctccatga
     2341 gattcgcgcc gagcaggttc aggaggcgcg gcacccccca caccgcctgg taggtcgcca
     2401 ccacgctcgt gccggaggcc atgggcggga tgcgcgggaa ccagcgccac acgcagccgg
     2461 ccaggagcag catcacgagg ccgatggccg cgaaggccca gcgccacccg aacccgccgc
     2521 cgagcgcgcc catgaccggc acgccgccga tggccgcgaa gctgaagccc atgttgaacc
     2581 agcccatggc gcgggcgcgg cgctcggccg ggaagaggtc gcccaccgag gccatgaggc
     2641 tgctgggcgc cagcgagccc agcaggccgg ccaggaagcg gatcgcgacg agcgccgcga
     2701 agccgggcgt gagcgcggcc gtgaagctca gcacgcccac gctggcgaat gcggtgacga
     2761 tcatcggccg gcgccccagg cgatccgaca agagccccga gaacggcgag aacagggctt
     2821 gcggcacccc ggccgctacg gccagcaggc ccgcctggct caccgagatc cccaggtccc
     2881 gcgagatgtc cacgagcagc ggcccgatca tcgtcaccga cgtgacgatt ccggtcacgc
     2941 ccgccgtgat cagcaccagg ttggggtcag gtcttacatt catacattcg ggtatacatt
     3001 cggcacgatc cagccctcgc gctcgcgggc gatccgctcg tcctcggcgg tgaggtcggg
     3061 cgggcgggcc agggatcggg ccagtgaatc gggcgcgtcg agcgcccagt cgcggaccgc
     3121 cgcgacctga caggcgtcct tgaccgggta gggctgccgg ctgcagtcca ccaggctcgg
     3181 ccagagctcg gcgatcacga tgctgtcgtc acccaccgcc gcgtcccacg cggtctcgaa
     3241 gggccagagc cgcgtgcgcg ccgccagcgc gccatccgtg agcaggcgat gcacggcggg
     3301 caggcccagg agcgtctgcg agccgaccgc cccgcgcgtg aagagcttcc acgggctctg
     3361 gatgtgccgt gacgccagcc gccgctcgac gagccggcca tcgcggatcc gccagggaaa
     3421 ggggcgcggg cgtgtccgga agaggtgtgg gtactcgtgg ccggccggat ggccccagaa
     3481 gggcccgtcg aagcccccac cgaatgcggc ctggatctcg cggttcagga tcccggcgac
     3541 ctcgaaccgg ttgttcgcgc cgtcgggcgc gtcgcgaatc aggccgtcga gccgggcgca
     3601 gagcgctcgc ccggcgggga gcccggcgtc ggccggatag ccgaacgcga agtcgaagcc
     3661 cacgagggcg gccccgggct cgcccaggag caggccgaag atgaacgcct ccgcctcggg
     3721 tcgggtgcgg aagtagcgtg gcgccggacg ttgctccccg gcgcgccccc acgcgaccca
     3781 gcaccggtcc cgcgcgggcc ggaccggccc ccgcgtcgag gccgccgacc agtccgcgac
     3841 gacgaccagc cggaagctca gggggtgagg acgatcttgc cgaactgcct gccctccgcg
     3901 aggcgccgct gcgcctcgac gccgtccttg agcggcatca ccgtgtccac gatggcccgc
     3961 agccgccccg cgaagagctg gcccatgacc gtgttgaact cggcgtcgtt cgacatcgtg
     4021 gatccgatga tcgagagctg cttccagaac acgagccgga tgtcggtctc ggcgatgggg
     4081 cccgaggtgg ccccgcaggt gacgatgcgc ccgcccttgg ccacggcgcg gatcgcgccc
     4141 ttccaggtgg cggcccccac gttctccagc acgacgtcca ccccacgccg gcccgtcttc
     4201 tggccgatca ccttcaccca gtcttccttc gcatagttga tgcactcgtc cgcgcccagc
     4261 gcccgcgcgc gctcgagctt gtcgtcgctg gaggaggtga cccacacgcg cgcgccgcag
     4321 agcttgccga tctgcaccgc ggtggacgag acgccgccgc ccaccccgat gaccaggagg
     4381 tcctccccgg cgcgcagccg cgcctgggtg accagcatgc gccaggcggt catgttcacg
     4441 aggatgaagg ccgccgcgtc ctcgtagcgg atcgcgccgg gcagcgggag aacgtggtgg
     4501 gcctccacct tcacgtactc ggcgagcccg ccgtgggtgt gctcgcccag gatgccgtat
     4561 ctcacgcaca tgctgtgctc gccgcgccgg cagtactcgc agcgcccgca cgagagattc
     4621 gggttcaccg cgacgcgatc gcctaccgtg acgtcccgca ccgccgcgcc cacctgggcc
     4681 acgtcgcccg cgatatcgca gcccgtccag aacggcaggg gcagggtgag gccgggcagg
     4741 ccctcgcgga cgaagagatc gaggtgattc agggccgccg cgcgcacacg gaccagcacg
     4801 tcgcccgcgc cgatggtcgg gtccgggacg tcctggtacc ggagcttgtc gaggccgccg
     4861 tgctcgtgga aggcgagcgc cttcaagagg cgccgagtcc cgccttcggc gcgttcacca
     4921 gcaccgccat gttgccgtac gggtgcttgt tgtccagcat gagctggtgg cacgcgggca
     4981 gctcgtcgaa gccgtaggtc tgggacaggc acgggtcgac cttgcccgcg tccaccagcc
     5041 gcgtcaccgc cagcgcctgc tggtcgttcg agaggtggct gccctggaag cgcttctggc
     5101 gcatccagtg atagcggaga tcgagcgtga cgttgtaacc ggtggtgccc gcgcagatca
     5161 cgatcatgcc gccggtgccg cagacgaact cggaggtggg cagggtggcc tcgcccggat
     5221 gctcgaagac gattctcggg ctggtgcgct cgcccagggc gtcccagatc gccttgccga
     5281 acgcccgcgc gcccttggcc cagttgccat aggccttgcc gtccttggta tcgggcatcg
     5341 ggccccagtg atcgaactgg gagcggttga tgacgcccac cgcgccgtgg tccatgcaga
     5401 acttcttctt ggcgtcgtcc gagaccaccg cgatcgcgcg gccgccctgc gcgcggacga
     5461 tctcgagcgc caggctgccg aggccgccgg tggcgcccca gaccagcacc acgtcgccct
     5521 tctcgacggt gtgcggctcc cagcccatca gcatgcggta ggcggtggag gcgcagagca
     5581 ggaagcagcc cgatgcctcc caggtgagcc gcttcggctt ggccaggcac tggtgcgcct
     5641 gggcgcgggc gaactggcag tagctgccgt agttggtctg gtagccccag atgcgggtgg
     5701 actcggccag catcgggtct ttcccggaca gcacccacgg gtcgtcgggc cgccaccagc
     5761 cgctgtgcac gatgacctcg tcgccgacct tgacgttctt cacgtcgtgg ccggtggccc
     5821 acacgatgcc ggagcagtcg ctgccgcccg cgtggaagtc ctcgggctcg cccatcttca
     5881 ggcgctccgc gatgacgtcg aggggctggc ccagcgcggc ccacacgttg ttgtagttga
     5941 tgcccgaggc catcacgtag atcagcacct cgtcggggcc gatggtgggc gtgggcagca
     6001 cctcgcgctt ccacgcctcg cgcggctggc cgaagcggtt ctgccggacc gcgaacgcgt
     6061 gcatccgctt cgggacctcg ccgagcggcg gcttgtcacc caggtcgtag agatcagcca
     6121 tgaatgcctc cgggcctatc ggcccttgaa gttggcgcgc ttccgctcgg cggcggcctt
     6181 gaggccggcc ttgcggtcct cggagaggag cgggcccgag gcctgcgccg tctcccagtc
     6241 gagccacgcg ccgatgtcgc cgtagtaggt gccgttgatt gcccgcttgg ccgcctggac
     6301 cgcgagcggg gcgttggccg cgatctcgcc ggccagctcg gccacacgcg cgccgagctc
     6361 gcccgccggg tacaccgcct ggacgagccc gatgcggtgc gcctccgcgg cgtcgaaccg
     6421 tcgcgccgag tagatcagct ccttggcctt cccgatgccg accgtgcgcg gcagccgctg
     6481 cgtgccgccc atgtcgggca cgaggccgag ctgcacctcc gggatggaga agagtgtgcc
     6541 ctcggcggcc aggcgcacgt cgcagcagag ggccagctcg aggcccgcgc ccatgcaggc
     6601 gcgctggatg gcgccgatga ccggtacccg gagcgcctcc accgcggaga acgacgcctg
     6661 atgcccctcc aggtgccggc ggatgcgggc gagcgcctgg ccgggagaca tgtcctccag
     6721 cgccgtgctg agcgtcgagc ccgccttcac gtcgagcccg gcgcagaagc aaccgcccgc
     6781 gcccatcagc acggccacgc gcacgtcggg cgctgcgtcc acgacccggc aggcctcgcc
     6841 gatgccgtgc cacatctcgt tgtcgagcgc gttcagtcgc tcgggcttgt tcagcatgat
     6901 ggtggcgatg tcgcccttga tctccacctg caccttgtcg gtcatcgctg gcccctatcc
     6961 attgaaggcc cgcaccatcg cgggcaggcg cggatcgttg aggttcggct ggtacggctg
     7021 atagagcccg gtgcgatcga gcaggccctg gtagcgctcc aggatcttcg cgtgcaggtt
     7081 ctcgtaggtc gcggtcaccg cgtaggtgtc gagcatctcg tcggtgatga ggtcggccat
     7141 ggccttccag tcgccctcca ccgacttgcg gtggagcttg ccggtgacgt cctcccagcc
     7201 gtgcgcggcc aggaccggct ggtaggtgcg cgtggagccg tagaaggcga tctgctgctt
     7261 cacggtctgg gcctgtgcgg cccgctcctt ctcggtgtcg cctaccacga cgaaggtggc
     7321 cgtcgcgtag gtgaagtccc ggcgcgagcg cccggagcgc ttcagtcctt cctccaccgc
     7381 cgggtggacg atctcgcgga ggtacttcgg cgagttgaac gggtgcacgt gcatgccgtc
     7441 gcacagctcg cccgcgatcc ggcacatcag cgggttcacc gccgcgatgt agatcgggat
     7501 cttcgggtga tcgatgggcc cggggttgaa gaacggcgtc atcacgtcga aggtgtagaa
     7561 ctgccccttg aagttgagcc tgccgccggt ctgccagcag tcccagatgg tgcggatggc
     7621 gcggatggcc tccgccatct tcgggccggg cgactcgaac ttcaccgaga agcgccgctc
     7681 gttgtgcgcc ttcacctggg tgcccagccc gagcgtgaac cgtccggccg aggccttctg
     7741 caggtcccag gcgatgtgcg cagtgatcat cgggctgcgc gagaacaccg tggcgatgtt
     7801 ggtgccgagc ttgatccggg acgtgcaggt cgcggcgacg gccaccggca ggtacgggtc
     7861 gtgctgggtc tccgacgacc acaggcagtc gtagcccatg gcctcgacct tccgggcgta
     7921 gtcgggaatg gtcttgagat cgtgggtcag catggacacg tcgagcttca cgggcattct
     7981 ccttgctcgg gggttcggcc agaagcatac cttaccctcc tccatgattc accctctccc
     8041 cagaggggag agggcagggt gaggggacgg tcccgcccga ttccttgccc cgtcccgtct
     8101 aacgatatcg tgcccctctc ccgcggcctc gcggtcgccc gcagtgctcg ggggaaaggc
     8161 gctacggcgt tgacaaccgc cctggccgcg cctagcgtgt caccgcatcc agcccgagcc
     8221 ggcgggagcg gcggggcaca agggggaaca acgcgatggc gcagaagaaa ggcaccgggc
     8281 tcctgatggt gtgggcggac gtgccggcgg acaaggaaga cgagttcaac cgctggtaca
     8341 acgaggagca tctccccgag cgcatggcca tccccggctt cctgagcggg gcccgctacg
     8401 aggccgtgaa gggcgggccc aagcacctcg cctactacga gctggagagc gcggcggtcc
     8461 tggagacccc ggcctacaag aaggtgcagg ccaatcccac cgagtggacg aagcggtccg
     8521 ggccggggac gatcgcgacc acgttcatcc gcaacgtcta caccatgatt catcccacgg
     8581 ctttgacgcc cgagattggg ggggcaccga tggcgccggt gctccagatc gggcgcatgg
     8641 acgtgccgcc cgccgccgat cgcgagttca acgactggta caacacggtc tacgtgccga
     8701 actacgagaa ggtgcccggg gtgatccgcg gccggcgcta tcgcgcggtg gagggctcgc
     8761 ccacctacct cacgctctac gagttcgagc accccaaggt ctcggagagc cccgcctgga
     8821 tggcccagcg cgatgcctcg ccggtgaacg cgcagatgcg gaagcacatg aagcacgcgc
     8881 ccggctcgcc gggggtctac gtcaagacgg tcgagctccg atagtcagcc ttaccgtcgt
     8941 cccttcaggc gggtgcaggc ttccgacggc ggcaccgaac accacgaggc gagtaacgac
     9001 gggactcaaa tccttggccc ctgatacaat gccggcggca ggcagtcaag ggcgcgttcc
     9061 ccaccactcg ggaggcctcc catgtacgac tacgtgatcg tgggtgccgg ctccgccgga
     9121 tgcgtcctcg cgaatcgcct ctccgccgat cccgccacgc gggtcctgct gctcgaggcc
     9181 ggcggacccg acacctcgcc cttcatccac acgcccgcga tgatggggtt cctgccggac
     9241 ggcccccacg actggcgata ccgcacggtg ccgcaggtcc actgcaacct gcggcgcttc
     9301 gcgtggccgc gcggccgcac gctcggcggg tccagctcga tcaactacat gatctacatc
     9361 cggggccacg ccgccgacta cgaccactgg gcccggctcg gcaacaaggg ctggagctac
     9421 gaggacgtgc tgccgttctt ccggaagtcc gagcacaacg agcggctgga agaccggttc
     9481 cacggtcgcg gcggcccgct caacgtggcc gatcaccgat tccgccaccc gctctcggag
     9541 atgttcgtgg cggccgccga ggagaccggc gtgccgccga acgccgactt caacggcgcc
     9601 gtccaggacg gcggcggctt ctaccagctc acccagaagg acgggcggcg ctggagcacg
     9661 gccagcgcct tcctgcggcc cgcgctggag cgcccgaacc tgaccgtcat caccggtgcg
     9721 ctgacgtgtc gcatccggat cgaggggcgg cgcgcggtcg gtgtggaata tgtccatcac
     9781 gggcagtcgg cgcgggccga cgccttgcgc gaggtagttc tctgcggcgg cagcgtcaac
     9841 tccccccagc tcctcatgct gtccgggatc gggcccgccg agcatcttcg cgccgtgggc
     9901 gttccggtgg tcgccgacct gcccggcgtc ggccggaacc tgcaggatca tctcgggacg
     9961 ttcctgcgca acgagatcac ggtgccgttc tcgctctacg gggccacgcc cgagcagctc
    10021 gcggccgtcc agaccgagta cgagaccacc ggccaggggc tgttctcgtc gaacatcgcg
    10081 gaagcgggcg cgtttctcag gacggacggc accgacggcc cgcccaacct gcagggcttc
    10141 ttcctgccct actgcatcac cgaggcgccg ctcgaggcct tccagcccta ctgccacggc
    10201 atcaccttcg cgttctacgt caaccggccg gcgacccgcg ggcagatcac gctggcgtcc
    10261 gccgatccgc tcgaccagcc gctgatcgat ccggcatacc tgagcgcgcc gtccgatctg
    10321 ccgcaattcg tcgccggcat ccggatgctg cgcgagatct tccgcgcgaa gccgctcggc
    10381 gacatcctgg ggcgcgagct gggccccggc agcgaggcgc ggaccgacgc ggagatcgcc
    10441 gcctacgtgc gcgaccgggc ctcgggcacc atcttccacc cggtgggcac gtgcaagatg
    10501 ggcacggacg agatggcggt ggtggatccc gctctccgcg tccacggcct cgacggcctg
    10561 cgcgtggtgg acgcctccat catgcccacg ctcatcggcg gcaacaccaa cgcgccgacg
    10621 atcatgatcg cggagaaggc ggcggcgatg atggtgggcg gctagcggat gcgatgcaac
    10681 gggtgaccac ggtctccgcg gggcaggccc cacggcggct cgacgtcttc gtggccaccc
    10741 agaccgcgca cctctcgcgg gccgccgccc agcggtggat cgacggcggt ctcatcaccg
    10801 tcaacggccg gcagaccaag cccgcgcgcc tgattcgccc gggcgacgtg atcacgtgtc
    10861 acgtgccggt gcgcgagccc ccgccggtcg agcccgagcc gctcgcgctg cgcgtgctcc
    10921 acgaggacgg ggagatcctc gtgatcgaca agccgccggg tctcgtgatg caccccggtc
    10981 ccggccactg gacgggcacc ctgctcaacg cgctggtcta tcacgtcggc gacgagcgcg
    11041 gcgccgggcg gcgcgcccgt cccggcctgg tccaccgcct cgacaagggc acgagcgggg
    11101 tcctcgtgat cgcgaagacc gacgcggccc atcgcgatct ctcgcgccag ttccgcgccc
    11161 actccgtgca tcgcgtctat ctggcgctgg tgtcgggcgc ggtccgacgg ggcggcctga
    11221 tcgaccagcg gctgggacgg gacacgcgcg atcggcgccg ggtctcgacg cgatcgcccg
    11281 ccacgagacg ggcggtgacg gagtaccgcg tggccgagcg cctcggcccc gatgcgaccc
    11341 tggtcgaggc gcgcccgcac accggccgca cgcatcagat ccgggtgcac ctggcctcga
    11401 tcggccatcc ggtcctgggc gacgcgagct atgggacggc cccggccgat cccgcgctgc
    11461 cgcgcgcgat gctccacgcc gccgtgctgg gcttcgcgca tccggcgagc ggggagtacg
    11521 tggagttttg cgcgccgctg ccggccgacg tggagcacgc ggtggcgcgc tgccgcgcgg
    11581 gtgcttcccg ctgacgcgcg tcgaggcagg cggaagaaaa agctcgtctg gcttgacagg
    11641 gtttcgcagt ggccacgcca cgatccggcg cggctcatga aaagcaaagc tgagcgggcg
    11701 gcgccgggca gcggatgcgc gttcgcgagg cgagaaacgc cgaatcatgg cgcgctccga
    11761 gctcgctcga tctgggccct cccaggcgtc accgaaaccg gggtgtagag tcgtcccagc
    11821 aagaagggag actcgccatg gacacgcacc cgtcggatgt ccccatcatc tggcagccca
    11881 cgccggcccg tatcgcggag ctcgatcgcc tgggtgacga gatcgccgag ctctccgtcc
    11941 atctcgaggc cgcgacggcc cggctcctcg cgctcatccg cgagttcgac gcccgcggcg
    12001 gctgggccaa cggcttccgc tcctgcgccg agtggctcgc ctggcgcgtg ggcctcgccc
    12061 ccggcgccgc ccgcgaacat gtccgggtgg cccgcgctct ggggacgttg ccggccatgg
    12121 cggaggggct ggcgcggggg gagctctcct atgcgaaggt ccgcgccttg acgcgcatcg
    12181 cgaccccgga gaccgaggcg cggctgctgg cggtggggcg cgccggcacg gcggtccacg
    12241 tggagagcat cgtgcggggc tggcgtcagg tcgaccggca gaccgaagcc cgtcgggccg
    12301 cgcgccagca cgcgggccgc gccctgcacg tctaccagga tgaggatggc accgtggtcc
    12361 tccgcggacg cctgaccccg gaggcggggg cgctgctcct ccgagccctc gacgccgccc
    12421 gcgagacgct ctaccagcgg cgccgcgccg gccaggccgc cacaccggcc gccgacccgg
    12481 cgtcggagac cccgacccgg ccgcagcagc aggccgacgc gctggccctg ctggccgaga
    12541 cagccctgca tcacgagctc gatcccggag ccccgggaga acgctatcag gtggtcgttc
    12601 acgtggacgc ggcggtcctg gcgaacgcgg cccagcccgg gcaatcggtg ctggaggacg
    12661 gcccgcacgt tcccgcggga acgtcgcagc ggctggcctg cgatgcaagc cgggtggtga
    12721 tgcggcacga tgaggccggg cgggtcgtgg aagtcggggc ccgcacgcgg acgattccgg
    12781 ccgccctccg gcgtgcgcta cagcatcggg acaagacctg ccgcttcccg ggctgcacgg
    12841 tgcggatcgc cgaggggcat cacgtgcgtc attgggcgca tggggggccg acgaagctct
    12901 cgaacctcgc gttgctctgt cggcgccatc atcgcgccgt gcacgaggag ggctaccagg
    12961 ccgagcgcgg ggccgacggg acgctccggt tccgacgccc tgacggccgg ccgttgccga
    13021 atgtcccggc gccgccggtg gtagccgccg atccggccgg agcgatccgg gcgcagaacg
    13081 ccgcgcaggg cctcacgctt catgcgcgca ccgggctcgc ccagtggctc ggggagcggc
    13141 tggacgtgcg ctgggcgatc gacgtgctgc atccccgggc cgcgcgaatc gttcgacgag
    13201 agtcgccatc ccagccgggc taggtgagcc tggtccgcgg gtgtcaacgg tcgttgacag
    13261 gtgggggaat acgactcaga cctcgatctc gatccacgcg cggcgccgga tccggccggt
    13321 gaggcgggag aacaggtcgc cgatccacat ctgccagccg tactcggcct gtagcgcggc
    13381 gcgcgcgcgc tcgatcacgg cgggctccgt gacgatgcgc gcagccgcgt cgtaccatgc
    13441 gccgcgaacg tgcccgcgca tatcggagcg ggcgacccgc gcccgcgacg aatagcgcag
    13501 gcgcttgacc ttgcccgatt caccggccgt gaagacgtag agcttcccat ccactgccgc
    13561 gaaccagacc ggcgtcctga cctcggctcc gctgcgacgg aacgtggcaa ggctcagata
    13621 gcgatggcgg tcgagcgtgg cgatgtccgt cacggaggcc tgatgtcagg cgagcccgat
    13681 cgaccgggcc accgcctcga ggtgcagcgc cgcgtcgccc gcgtcgacac cggccgcgag
    13741 gatgcgcttg tggaagagat gcagcgggtg ctcctcgctg tagccgatgg cgcccatgat
    13801 ctggtggccc cggcgggcca cgcgcaggcc ggcctcggcg cagtaggcct tggccgcggc
    13861 gaccgcgggg tcggcgtcgg gccggccgga ttccagcctc cacgcggctt cccacatgag
    13921 ccaccgcgcg ctctcgacgt cgcgaaagag gtcggcgcag gcgtgctgga tggcctggta
    13981 gccgccgatg ggacggccgc cctgcacgcg gacgcgggcg tgctccacgc agagctcgag
    14041 cacgcgctgg gcggcgccca cgatctcggc gctccgggcg agggccccgg tgcggaacgc
    14101 gggagcgagc accatccagc cgcctccggg cgcgccgagc agatcgtccg gtgcggcctc
    14161 cacgtcggcg aggtcgagct cgaagagctt gtcgccggtc atgctgtcga gcgggcgcgc
    14221 ggtgatgccg gcgcggccca tcggcagcag gagcgcggtc ggcccggcgg cggcccggcc
    14281 gaggacgatc acgtcggttg ccacgtgcgc gtccgagacg aagagcttgc gtccggtgag
    14341 gcggccgggg gtggcgacgg gcagcgtcat cacgtgcggg tcgagtcgcc ccgactcctc
    14401 gagcacggcg agcgcgcagg tccgctcgcc gagggccagg gcggggagca gcgcccgggc
    14461 gcgcccgccg ccgcccgcgg tcagcgcggt ggtggcgacg acggcgctcg cgatgaacgg
    14521 gctcgggaag cacgcgcgtc ccatctcctc gacgagggcc agcgcatcgc cgatggagcc
    14581 accgctgccc cccaggtcgg tcgggaccag gaggcccggc cat
//
LOCUS       DASUFA010000194         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1168247, whole genome shotgun sequence.
ACCESSION   DASUFA010000194 DASUFA010000000
VERSION     DASUFA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168247"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VFX87_07030"
     CDS             <1..1219
                     /locus_tag="VFX87_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4994703.1"
                     /translation="SERRYPPALAGPRYPQGIAIREEAELEGICRRESVAEVVFAYSD
                     VSHESVMHAASRALAVGADFTLLGPRRTMLRARVPVIAISAVRTGCGKSQTARWLGRR
                     LRERGRRVAILRHPMPYGDLERERVQRFARRRDLDAGRCTAEEREEYEPHLEAGNVVF
                     AGVDYAAIVGAAEKEADLIVWDGGNNDFPFLVPDLHIVIADALRPGQVAGFHPGEAVA
                     RMADVVVINKVDAAPRADVERVSEEIRAVNPRATVVRAASPVRLDDPAAVRGRRVLVV
                     EDGPTITHGGMPYGAGYVAATAAGAAAIVDPRASAAPAIAALFERYPHIGRVLPAVGY
                     SGAQLEALRESINGARADVVVAATPIDLAGLISINKPVVRARYDFADAGEPTLGSVVD
                     AFLTRLAPGTDLE"
     gene            1222..>2042
                     /locus_tag="VFX87_07035"
     CDS             1222..>2042
                     /locus_tag="VFX87_07035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4994704.1"
                     /translation="MLVKALACDFDGTLASEDRIGAEARAALQQARAAGLRLILVTGR
                     TFFELTRVCDCLDLFEAVVAENGAVLYYPAESMIRDQAPPPPGRLLSELDRRGIYYQA
                     GRVIVGTARSDEGAVREALAAADATREIVYNRGALMLLPPGLSKGTGVQQAVRYLGLS
                     PHDVLALGDAENDLPLFEACGWCGCPASADPEVRARADWIFPGEDGPSIAAAITGPIL
                     GGALPPAPALRHQIVIGWVVTTSEPVTVPVRDVNVLIHGDPQSGKSWLAGALMERL"
BASE COUNT          253 a          725 c          768 g          296 t
ORIGIN      
        1 cagcgagcgg cgttatccgc cggcgctggc gggacctcgt tatccccagg gcatcgccat
       61 ccgggaggag gccgaactcg aggggatctg ccggcgagag tccgtcgccg aggtggtctt
      121 cgcctacagt gacgtgtcgc atgagagcgt catgcatgcg gcgtcgcgcg cgctcgccgt
      181 cggcgcggat ttcaccctcc tgggcccgcg ccgcaccatg ctccgggccc gcgtgcccgt
      241 catcgccatc tcggcggttc gcaccgggtg cggcaagtcc cagaccgcgc gctggctggg
      301 ccgtcggctc cgcgagcggg gccgtcgcgt cgcgatcctc cgccacccca tgccgtacgg
      361 tgatctcgag cgtgagcggg tgcagcgctt cgcccggcgc cgcgatctcg atgccgggcg
      421 gtgcacggcc gaggagcggg aggagtacga gccgcacctc gaggccggga acgtggtctt
      481 cgcgggcgtg gactacgcgg cgatcgtcgg cgccgcggag aaggaagccg acctcatcgt
      541 gtgggacggg gggaacaacg actttccctt cctcgtgccg gacctccaca tcgtgatcgc
      601 ggatgccctg cgccccggcc aggtggcggg cttccacccg ggcgaggcgg tggcccggat
      661 ggccgacgtc gtggtgatca acaaggtgga tgccgccccg cgcgcggacg tcgagcgggt
      721 gagtgaggag atccgcgccg tgaacccgcg cgcgaccgtg gtgcgcgcgg cctcgccggt
      781 gcgcctcgac gatcccgcgg cggtgcgcgg ccggcgcgtg ctcgtcgtcg aggacggccc
      841 cacgatcacg cacgggggga tgccgtacgg cgccggctac gtcgccgcga cggcggcggg
      901 cgccgccgcc atcgtggatc cgcgcgcgtc ggcggccccc gccatcgcgg cgctcttcga
      961 gcgctacccc cacatcgggc gggtgctgcc cgccgtcggc tacagcgggg cccagctgga
     1021 ggcgctgcga gagagcatca acggggcgcg cgccgacgtg gtggtggccg cgaccccgat
     1081 cgacctcgcg gggctgatct cgatcaacaa gccggtggtg cgcgcccgat acgacttcgc
     1141 ggatgccggc gagccgacgc tcggatcggt ggtcgacgca ttcctgacgc ggctcgcgcc
     1201 ggggaccgat ctcgagtgac catgctcgtc aaggcgctcg cgtgcgactt cgacggcacg
     1261 ctcgcgtccg aggaccggat cggcgcggag gcgcgggcgg ccctccagca ggcgcgcgcg
     1321 gccggcctgc ggctcatcct ggtcaccggc cgcacgttct tcgagctgac ccgcgtgtgc
     1381 gactgcctcg acctgttcga ggcggtggtg gccgagaacg gcgccgtgct ctactatccc
     1441 gccgagtcca tgatccgtga ccaggccccg cccccgccgg gccggttgct ctcggagctg
     1501 gatcgacgag gcatctacta tcaggccggc cgggtcatcg tgggcaccgc ccgatcggac
     1561 gagggtgcgg tgcgggaggc gctggcggcg gcggacgcca cgcgcgagat cgtctacaac
     1621 cggggcgcct tgatgctcct gccccccggc ttgtccaagg gcaccggcgt gcagcaggcg
     1681 gtgcgctatc tcgggctgtc gccgcatgat gtcctggccc tgggagacgc cgagaacgat
     1741 ctgccgctgt tcgaggcctg cggctggtgc ggctgcccgg cgagcgctga tccggaggtc
     1801 cgggcgcggg cggactggat cttccccggc gaagacggac cgagcatcgc cgccgccatc
     1861 accgggccca tcctgggcgg tgcgctcccg ccggcgcccg cgctccgcca ccagatcgtg
     1921 atcggctggg tggtgaccac gtccgagccg gtgacggttc ccgtgcggga cgtgaacgtc
     1981 ctgatccatg gagatcccca gtcgggcaag tcctggctcg ccggcgcgtt gatggagcgg
     2041 ct
//
LOCUS       DASUFA010000195         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_848013, whole genome shotgun sequence.
ACCESSION   DASUFA010000195 DASUFA010000000
VERSION     DASUFA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848013"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFX87_07040"
     CDS             <1..1081
                     /locus_tag="VFX87_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX4994705.1"
                     /translation="TITGSHNPVSDNGVKMVHAGAAPLTESEIQGLLATIQSGDFERG
                     VGTRATRDPRDEYWDAIAARVTLRRRLKVVVDAGNGIAGVFAPELLRRLGCEVVELYC
                     ESDGSFPNHLPDPEMEENMRALVARTLEVGADVGVAYDGDADRVGIVDERGRRHEADL
                     LLALLARDLLTRHPGAKIVFDVKCSQVLVDDITKHGGEPIMWKTGHSLLKRKMREDGI
                     LLGGEVSGHMFFGEDWYGVDDGILASCRFLQLVASGAGPASAHFDTLPHLHATPELKA
                     PCPDDKKFAVVDALAREFRSRFETIDIDGARILFPEGWGLVRASNTNPYLTLRFEGRS
                     ADAVLNMQRMVYDALRRFPYVTLPA"
     gene            complement(1085..1801)
                     /locus_tag="VFX87_07045"
     CDS             complement(1085..1801)
                     /locus_tag="VFX87_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4994706.1"
                     /translation="MNRPGSPARPPLRAVIFDAGNTLLRMNYGSIADHLRTRGRAASP
                     EEVEEAELRARVRLDPHLAPGAASTESTVTHGRYLRYLLENLAITDDAEIQAIAQWRR
                     GYNLPAGLWNRADPGALAAIHRVRGAGLVAGVISNSNGSVRAILEETGLAAHLAFIID
                     SSVVGVEKPDPRIFHLGLREAGVAPAEAVYVGDLYSVDVLGARAAGLDGILLDPGGFW
                     APRDCRLARGLEDAVRLALD"
     gene            1842..2036
                     /locus_tag="VFX87_07050"
     CDS             1842..2036
                     /locus_tag="VFX87_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994707.1"
                     /translation="MRAALLLLATTAWADHGGPLRAEGLSPLTVGLLAGALALAAGVL
                     VVVIVMLLTRKPSSPAHKPE"
     gene            2033..>2490
                     /locus_tag="VFX87_07055"
     CDS             2033..>2490
                     /locus_tag="VFX87_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994708.1"
                     /translation="MTAFGIAARWLHLACVLGLVGLVTALLLVGRSDRPTALAWEARM
                     LRWARGLTGLLLVSGLAALAYQAAVVTGRAGAALDPVAWALLLGRSQFGTVWLVRHGL
                     LLLLAALLLLREREQSSVDRLAFRGEAWLLAAVGAGAMAWAGHAAAVEPQG"
BASE COUNT          314 a          926 c          896 g          354 t
ORIGIN      
        1 caccatcacc ggcagccaca atccggtctc ggacaacggg gtcaagatgg tgcacgcggg
       61 cgcggcgccg ctgaccgaga gcgagatcca ggggctgctc gccaccatcc agtcgggcga
      121 tttcgagcgg ggcgtgggca cgcgcgccac ccgcgatccg cgcgacgagt actgggacgc
      181 gatcgcggcc cgggtgaccc tgcggcgaag gctgaaggtc gtggtggacg cgggcaatgg
      241 gatcgcgggc gtcttcgccc ccgagctgct gcgccgtctg gggtgcgagg tggtggagct
      301 ctactgcgag tccgacggca gcttccccaa ccatctcccc gatccggaga tggaggagaa
      361 catgcgggct ctggtggccc ggactctcga ggtcggcgcc gacgtgggcg tggcctacga
      421 cggcgacgcc gaccgggtcg gcatcgtgga cgagcgggga cggcgccacg aggccgatct
      481 gctgctggcc ctcctcgccc gggatctcct gacccggcac ccgggggcca agatcgtctt
      541 cgacgtcaag tgctcgcagg tgctggtgga cgacatcacg aagcacggcg gggagccgat
      601 catgtggaag accggccact cgctcctcaa acggaagatg cgcgaggacg ggatcctgct
      661 cggcggcgag gtctccggcc acatgttctt cggcgaggac tggtacgggg tggacgacgg
      721 catcctcgcc tcgtgccgct tcctgcagct cgtcgcgtcg ggcgcggggc ccgcctccgc
      781 gcacttcgac acgctgccgc atctgcacgc cacgccggag ctgaaggcgc cgtgccccga
      841 cgacaagaag ttcgcggtgg tggacgcgct ggcccgggag ttccggagcc gcttcgagac
      901 catcgacatc gacggggccc gcatcctctt cccggagggc tgggggctcg tgcgcgcgtc
      961 gaacaccaat ccgtacctga ccttgcgctt cgagggccgc tccgccgacg ccgtcctgaa
     1021 catgcagcgc atggtctacg acgccctccg ccgcttcccc tacgtcaccc tccccgcctg
     1081 agccctagtc cagggccagc cgcaccgcat cctccagccc gcgcgccagc cggcagtcgc
     1141 gcggcgccca gaagccgccc gggtcgagca ggatgccgtc gagcccggcc gcgcgcgcac
     1201 ccaggacgtc caccgagtac agatcgccca cgtagaccgc ctccgccggg gcgacgccgg
     1261 cctcgcgcag ccccagatgg aagatgcggg ggtcgggctt ctccacgccc accacggagg
     1321 agtcgatgat gaaggcgaga tgcgcggcca gcccggtctc ctcgagaatg gcccgcaccg
     1381 agccgttgga gttcgagatg accccggcca ccagcccggc cccccgcacc cggtggatcg
     1441 cggcgagcgc cccggggtcg gcgcggttcc acaggcccgc cggcagattg tagccgcgcc
     1501 gccactgggc gatcgcctgg atctcggcgt cgtcggtgat cgccaggttc tccagcaggt
     1561 agcggagata gcgcccgtgc gtgaccgtgc tctcggtgga ggcggcaccg ggcgccaggt
     1621 gcggatcgag ccgcacccgc gcccgcagct ccgcctcctc cacctcctcc gggctggcgg
     1681 cgcgcccgcg cgtgcgcagg tggtcggcga tgctgccgta gttcatgcgc aggagcgtgt
     1741 tgccggcgtc gaagatgacg gcgcggagcg gcgggcgcgc gggtgatccg gggcggttca
     1801 tgggggcggg ctctccggca ctcattataa tgaggccgcc catgagagcg gccctcctcc
     1861 tcctggcgac cacggcgtgg gccgaccacg gcggtccgct gcgcgcggag ggcctgagcc
     1921 cgctcacggt cggcctcctg gcgggcgcgc tggccctggc cgcgggcgtg ctcgtcgtgg
     1981 tcatcgtgat gctcctcacg cgcaagccgt cctcgcccgc ccacaagccc gaatgaccgc
     2041 cttcggcatc gcggcccgct ggctgcacct ggcctgcgtc ctcggcctgg tgggcctcgt
     2101 caccgcgctg ctcctcgtcg ggcgcagcga ccggccgacc gcgctcgcct gggaagcgcg
     2161 catgctccgc tgggcccgcg ggctcaccgg gctgctcctg gtctccgggc tcgccgcgct
     2221 cgcctatcag gctgcggtcg tcaccggccg cgccggcgcc gcgctcgatc cggtggcgtg
     2281 ggccctcctc ctcggccgga gccagttcgg caccgtctgg ctggtgcgcc acggcctgct
     2341 cctcctgctc gccgcccttc tcctgctgcg cgagcgcgag caatcctccg tcgaccggct
     2401 cgccttccgc ggcgaggcgt ggctgctcgc cgcggtgggc gcgggcgcca tggcgtgggc
     2461 cggccacgcg gcggcggtcg agccgcaggg
//
LOCUS       DASUFA010000196        12720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_728326, whole genome shotgun sequence.
ACCESSION   DASUFA010000196 DASUFA010000000
VERSION     DASUFA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12720
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728326"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFX87_07060"
     CDS             <1..198
                     /locus_tag="VFX87_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein"
                     /protein_id="HEX4994709.1"
                     /translation="ANIEQVAFRPSSDAPPQRFTVSVGKVSFPQDGHDHAALLSQALA
                     FLDRATHRGGNQVTDSGGILD"
     gene            337..915
                     /locus_tag="VFX87_07065"
     CDS             337..915
                     /locus_tag="VFX87_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994710.1"
                     /translation="MPPVDPNEVMMTGENSFIRLSPDGGKTLSDRASHWRVLWCPAGG
                     GHALFLQSTLTDGRVRIYSDNIAVARWLQKSIETLLFPAFADTSIPVMAARFERQGGP
                     WSSATETVLAVDARITMTWYDCIEPFVLNAPPGFNDRPIGVFSTFFPARAAQLELNGK
                     VAPGNVWPEMRGDRQGSSATLAWSETWVKPRG"
     gene            929..1801
                     /locus_tag="VFX87_07070"
     CDS             929..1801
                     /locus_tag="VFX87_07070"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994711.1"
                     /translation="MSGRLDGKVAIITGGGSGIGRAATLTFLRESARVVIGDLNEAGL
                     AETVALARAQGHADRLAALRGDVSAEGHVADLVALAVERFQGLDCVFNNAGVGGAFGP
                     IGETTAEEWDFTFAVLVRGVFLGIKHASNRMKAQGRGGSIISTASVAGIGGGGGPHAY
                     SAAKAAVVNLTRSVSTELAAHRIRVNAIAPGMIRTPLATGHREEAWGRLVREKQPWPE
                     LGLPQHIADTALFLAGEESRFITGQVIVVDGGLTAQGPDLFGHDAGSRMLRKAGLNTG
                     STGVPGTVREVPRP"
     gene            1851..1997
                     /locus_tag="VFX87_07075"
     CDS             1851..1997
                     /locus_tag="VFX87_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994712.1"
                     /translation="MAQGAVTAALSVRTAPVERIAGDGGVPSPGVEVNQVFGSLTEAR
                     GGPR"
     gene            complement(2010..2777)
                     /locus_tag="VFX87_07080"
     CDS             complement(2010..2777)
                     /locus_tag="VFX87_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994713.1"
                     /translation="MARLDGRGAAVTGGASGIGEATARRLAEEGAHVAIADIDAERGR
                     VVTDEIRVKGGRAIFVETHTEREFECVRFIRAARDEFGRLDVLVNNAGMRLYQTVVDA
                     SEESWDAILAVNVKGYAFCAKAAIPLMRQTGGGSIVNVASIRSVVAGGNMVQYDTTKA
                     AVAGLTRAMALDHAGDGIRVNAVCPGPIFTRFHERRAATAGKTFEQFRAEFGRGTMLR
                     RPGTPAEVAACILFLASDDASYVTGTSLYVDGGQVSL"
     gene            complement(2792..3979)
                     /locus_tag="VFX87_07085"
     CDS             complement(2792..3979)
                     /locus_tag="VFX87_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX4994714.1"
                     /translation="MSKTRPEWSQEITKRADDLVALRRDFHRHPELSHQERRTAEIIA
                     ERLHAAKLDVRTGVGGTGVVGLLHGDRPGPTIAWRADIDALPLTELLDAPFTSGTPGV
                     MHACGHDGHTAIAITMADILAARRAELPGTAVFLFQPAEEVLGGARPMIEAGALDNPR
                     VDEIYGLHLTTQQPVGQVQVRPGPSMASADAFTVEVRGAGGHGAMPHLSVDPITAAAS
                     ILLGMQALISREVPAQDTAVLTVGQIVSGTKGNIIPDRAIMRGTIRAFESSVRDHLVA
                     RLGAFAADIAKAYRAEATTRFDGGSCPAVVNHAKETEFVRTCAVDEVGGEAVTEGRVV
                     MASDDMSLFLRERPGCYFRVGIGPATGAARPHHAPEFEMNEAGLPVALRVGLSVMRAA
                     LAR"
     gene            4200..4529
                     /locus_tag="VFX87_07090"
     CDS             4200..4529
                     /locus_tag="VFX87_07090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4994715.1"
                     /translation="MSLAPRSSRRLHVRKNVSWTAWVTVGKTRIRCHTVDLSANGARL
                     KPRGDFQPGTPVQLQFQPPDGPMIGVAAVVWRVEAESMAVMFLRNIAVQVAVPSRMPE
                     HGRRGWR"
     gene            4656..5888
                     /locus_tag="VFX87_07095"
     CDS             4656..5888
                     /locus_tag="VFX87_07095"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4994716.1"
                     /translation="MTTRTLTDALATLLAVTLLIGCGSDYPAPGAAKSAPSGTAGAAI
                     PAKSPSVRVVPAMEERAAGVVVASGTLAAEEQVVLGMKVAGRLAEITVDLGTRVKRGD
                     VVARLDPTDFRLRVQLAENAIQQARARLGLPQDGASDRVDPDKTPLVRQAQAVLDEAL
                     LTRDRRIKLWEQQFIARAELDAAEAAAKVAESRYHDAVEEVNNRLGVLAEKRTELELA
                     RQQLADTVLRAPIDGAMNARQAAVGEFLNAGAPVATLVRIHPLRLRLAVPEREATAIR
                     QGQRVRLTLESDLTVSEGHVARLAPAISEQNRSLMIEAEIPNEQGRLRPGAFARGEIV
                     VQPDRKLVTVPAEAIVTFAGVEKVMVVEAGRAVEKRVTTGRRTGDRVEILSGLAAGEQ
                     VIVRPGAVASGQPVTASP"
     gene            5890..9099
                     /locus_tag="VFX87_07100"
     CDS             5890..9099
                     /locus_tag="VFX87_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4994717.1"
                     /translation="MQKLAEVCIRRPVFAAMLILALCVVGAASYFRLGVDRFPAVDLP
                     TVTVRTTLAGASTEEMEIQISQRIEEVVNTVAGISELRSISGPGSSVVIVTFELSRNI
                     DVATQDVRDKVATVVRNLPRDTLAPIISKSDNESSPVMTVALAGDRPLRELTELADKV
                     VKVRLERSSGVGEVRLVGGLERAISVWIDADRLAAYQIPISAVRAAIVRQNADLPGGN
                     VTEGAHESALRTMGRVMDPKAFDDLVIATINGVAVRVRDIGHAEDGTKEQRSIARLDG
                     VPTVTLEVRRQSGANTIAVIEAAKANLARIAGQLPPGVRLEVIRDQSRYIYAALREIN
                     VHLVLGAILASLVVLAFMRSWSSTLIAAVAIPASVISAFGMMWALNFTLNSVTMLALV
                     LMVGIVIDDAIVVLENIFRFVEEKRMEPFAAARAATADIGLAVLATTLSLVVIFVPVS
                     FMSSISGRFLYQFGLTAAVAVLVSLLVSFTLTPMMSARLLRAEARKRGPAGEGETHVA
                     VSRRGFYAWIDRGYAWALGLSMRHRIAVALIAALVIAATVPLYGLVRQEYIPTDVDEA
                     EFDVRVTAPQNVSVTAMDEVMQRVAQEITVAPGVRLVLASAGGGFLGQVNEGTVYVRL
                     APHGERTVTFARIWRGVISGDPLAGFRGLWGQRDVMQEVRRRLARIPDLRTSVRNAPS
                     FNTGGGSFDIDFIIRGPDLNALARYGEALRERARQLGGIADPDTTLKLDKPELRVEID
                     RARAADLGVDTEQVAETLRLMVGGDQEVSRFLDPTVNDDYDVQLRVAAGDRRDPGAIA
                     RFYVPRQNGGGLVRLDSVVKIVPAEAASRIDRSDRQRENRLRASVLPGFGQADRIQAL
                     RSAVAEMNLPTAYTTAIAGRARELERTFVEFLWVFLLSVIFMYMILASQFESLVDPLT
                     ILISLPISVPFALLSLWLTSHTLNLYSALGILVLFGVVKKNSILQIDHMNTLRAHGME
                     RLAAIMQANRDRLRPILMTTLALVAGMLPLAVGSGPGAEERRAIAVVVIGGQTLSLLL
                     TLLVTPVAYSLFEDLARMLGWQSLVRIGRRGLRAARARR"
     gene            9307..9603
                     /locus_tag="VFX87_07105"
     CDS             9307..9603
                     /locus_tag="VFX87_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994718.1"
                     /translation="MNKLIVAALVLLAAVAQVSVAAAQISAPAPTAPNSQGLPWWFVK
                     HQYTPGMPWFYRAPGAPAARQCRVAYWYPAQFWNTDQWGNTVTWTSYAPQYVCD"
     gene            9655..10671
                     /locus_tag="VFX87_07110"
     CDS             9655..10671
                     /locus_tag="VFX87_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994719.1"
                     /translation="MGAQATWREVIPEGEAARLEALAKRLLGRRRLDGRPLHRKTLAA
                     LHARFEVAEAPRDLRHGLFAEPGSYDTFVRFSSGAGRSRSDRVPDARGLAVKILGVTG
                     PKVIPGLETASTQDFLAILSPTFPFKDPAAFVDIAVASRRGKVAVVRAALRHYGPLGL
                     FGALPRLQRALDSSMRSLAERTYFTAVPLRLGPHAVKLRFRPMDVPPPVPPVGGPDAL
                     AVELQARVRASPLSFRVEAQRFVDELRTPIEDPTVEWTERDAPWVALARLAIPAQAAE
                     SRAGRALAAYVERLSFDPWHALVEHRPLGVVMRARAVAYKRAVIARGAISELEVKPPS
                     AVMG"
     gene            complement(10684..11388)
                     /locus_tag="VFX87_07115"
                     /pseudo
     CDS             complement(10684..11388)
                     /locus_tag="VFX87_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            11494..>12720
                     /locus_tag="VFX87_07120"
     CDS             11494..>12720
                     /locus_tag="VFX87_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX4994720.1"
                     /translation="MNGAESLARTLVAGGVNVCFTNPGTSEMHFVAALDKVDGIRCVL
                     GLFEGVVTGAADGYWRMADKPAATLLHLGPGLGNGLANLHNARKASSGIVNIVGEHAT
                     YHVKHDAPLTSDIEGIARPVSAWVRTAPSARAVAADGAAAIAAARTAPGQVATLILPA
                     DTAWNEADGIAAVPPVAARASVGTEAVERCAKALRSGEPAMLMLTGVAVRERGLALAG
                     RIATATGARIIAQGSNARIQRGAGRVSIERLPYPVDQALAVLKDVRHLILVGAKAPVA
                     FFAYPDKPSVLTPDGCQVHRLSTLAEDSMGALEALAEAVGAKKNGEVVQQPGRPALPT
                     GALTPDTLAAALGALLPAGAIVSDESVTTGRGFFKMTAGAPPHDWMNNMGGSIGLGLP
                     LATGAAVACPDRKVVCL"
BASE COUNT         1706 a         4702 c         4483 g         1829 t
ORIGIN      
        1 gccaacatcg agcaggtggc gttccggccg agctccgacg cgccgcccca gcgcttcact
       61 gtgagcgtgg ggaaggtgtc gttcccgcag gacggtcacg accacgccgc gctcctgtcc
      121 caggcgctcg ccttcctgga ccgggccacg catcggggcg gcaatcaggt gaccgacagc
      181 ggggggatcc tggactagac tcctcggtcc gccgcgcgga agcccggcgc ggctcgtgct
      241 ccccgggggt gagcgcgccg gcccccgcct cttgccccgg gcccgcttcg cccgtatctt
      301 catcaggccc cacaccacat tcacaggaga cgaccgatgc cgccagtcga cccgaacgaa
      361 gtgatgatga ccggtgagaa ttccttcatc cggctgagcc ccgacggcgg caagacgctg
      421 tccgaccgcg cgagccactg gcgcgtgctg tggtgcccgg cgggcggcgg ccacgcgctg
      481 ttcctccaga gcacgctcac cgacggccgg gtccgcatct acagcgacaa catcgcggtg
      541 gcgcgctggc tacagaagtc catcgagacc ctgctcttcc ccgccttcgc ggacacgtcc
      601 attccggtga tggccgcgcg cttcgagcgg cagggcgggc cctggtcgag cgccaccgag
      661 acggtcctgg cggtggacgc gcggatcacg atgacctggt acgactgcat cgagcccttc
      721 gtgctcaacg cgcctcccgg cttcaacgac cggccgatcg gcgtcttcag caccttcttc
      781 ccggcgcgcg cggcccagct cgagctcaac ggcaaggtgg cgccgggcaa cgtgtggccg
      841 gagatgcgcg gcgaccggca gggcagcagc gccaccctgg cctggtccga gacctgggtg
      901 aagccgcgcg gctagcggag aggcacgggt gagcgggcgg ctcgacggca aggtcgcgat
      961 catcaccggg ggcgggagcg gcatcggccg cgccgccacg ctcacgtttc ttcgagagag
     1021 cgcccgggtg gtgatcggcg acctgaacga ggcggggctc gccgagaccg tcgcgctcgc
     1081 ccgcgcgcag gggcacgccg atcggctggc cgcgctgcgc ggcgacgtct cggcggaggg
     1141 ccacgtggca gacctcgtcg ccctcgcggt cgagcgcttc caggggctcg actgcgtctt
     1201 caacaacgcg ggggtcggcg gcgcattcgg ccccatcggc gagaccaccg cggaggagtg
     1261 ggacttcacg ttcgcggtgc tggtgcgtgg cgtgttcctg ggcatcaagc acgcgtcgaa
     1321 tcgcatgaag gcccaggggc gcggcggcag catcatcagc accgcgtcgg tcgcgggcat
     1381 cggcggcggc gggggcccgc acgcctactc cgcggccaag gccgcggtgg tcaacctcac
     1441 ccgctcggtc tccaccgagc tggccgccca ccgcatccgg gtcaacgcga tcgcccccgg
     1501 catgatccgg accccgctcg ccaccggcca tcgggaggaa gcctggggcc gcctggtgcg
     1561 ggagaagcag ccgtggccgg agctgggcct gccgcagcac atcgcggaca ccgcgctctt
     1621 cctcgccggc gaggagtccc gcttcatcac cggccaggtg atcgtggtgg acggcgggct
     1681 gaccgcgcag ggccccgacc tcttcggcca cgacgcgggc agccgcatgc tccggaaggc
     1741 cggactcaat acgggcagca ccggcgtgcc cggcacggtg cgcgaggtgc cgcgccccta
     1801 gccggccgcg ccgtcgggcg gagtgccatc tccactgcgg ggccctggcg atggcccagg
     1861 gtgcggtgac cgcggcgctc agcgtgcgga ccgcgccggt cgagcgcatc gcgggcgacg
     1921 ggggcgtgcc ctcgcccggc gtggaggtca atcaggtgtt cggctcgctg accgaggcga
     1981 ggggtgggcc gcgctagtcg agcagaatct cagagagaca cctggccgcc gtccacgtag
     2041 agcgaggtgc cggtcacgta ggaggcgtcg tccgacgcca ggaagagaat gcacgcggcg
     2101 acctcggccg gcgtccccgg ccgcctgagc atggtgccgc ggccgaactc ggcccggaac
     2161 tgctcgaagg tcttccccgc ggtggcggcc cgccgctcgt ggaagcgggt gaagatcggg
     2221 ccggggcaca ccgcgttcac ccggatcccg tcgcccgcgt gatcgagggc catcgcgcgg
     2281 gtcaggcccg ccaccgccgc cttggtggtg tcgtactgca ccatgttgcc gcccgcgacc
     2341 accgagcgga tcgaggcgac gttgacgatg ctgccgccgc cggtctggcg catcaggggg
     2401 atcgccgcct tcgcgcagaa cgcgtagccc ttcacgttca ccgccaggat cgcgtcccag
     2461 ctctcctcgc tcgcgtccac caccgtctgg tagaggcgca tccccgcgtt gttgacgagc
     2521 acgtccaatc ggccgaactc gtcgcgggcg gcgcggatga aacggacgca ctcgaactcc
     2581 cgctcggtat gcgtctccac gaagatggcg cgaccgccct tcacgcggat ctcgtcggtc
     2641 accacgcggc cgcgctccgc gtcgatgtcc gcgatggcca cgtgcgcgcc ctcctcggcc
     2701 aggcgccgcg cggtcgcctc cccgattccg gaggcgccgc cggtgaccgc cgcccccctg
     2761 ccgtcgagcc gggccatgcg cccgccgcgc ctcagcgcgc cagcgccgcg cgcatgacgg
     2821 acagccccac gcgcagcgcc accggcaggc ccgcctcgtt catctcgaac tcgggcgcgt
     2881 gatgcggccg ggccgcgccg gtcgccgggc cgatgccgac gcgaaagtag cagcccgggc
     2941 gctcgcgcag gaagaggctc atgtcgtcgc tggccatgac cacgcgcccc tcggtcaccg
     3001 cctcgccgcc cacctcgtcc accgcgcacg tccgcacgaa ctccgtctcc ttcgcgtgat
     3061 tgaccaccgc ggggcacgag ccgccgtcga agcgcgtggt ggcctccgcg cggtaggcct
     3121 tcgcgatgtc ggccgcgaag gcgccgagcc gggcgacgag gtgatcccgg accgacgact
     3181 cgaaggcgcg gatggtgccg cgcatgatcg cgcggtccgg gatgatgttg cccttggtgc
     3241 cggagacgat ctggcccacc gtgagcaccg cggtgtcctg ggccgggacc tcgcgcgaga
     3301 tcagggcctg catgccgagc aggatgctgg cggcggcggt gatggggtcg acggagagat
     3361 gcggcatcgc gccgtggccg ccggcgccgc gcacctcgac ggtgaaggcg tccgccgagg
     3421 ccatggacgg cccggggcgc acctggacct ggccgaccgg ctgctgggtc gtgaggtgca
     3481 ggccgtagat ctcgtccacc cgcgggttgt cgagcgcgcc ggcctcgatc atcgggcgcg
     3541 cgccgcccag cacttcctcg gcgggctgga agaggaagac ggccgtgccc ggcagctccg
     3601 cgcgccgtgc ggccaggatg tcggccatcg tgatcgcgat cgcggtgtgg ccgtcgtgcc
     3661 cgcacgcgtg catcacgccc ggcgtgccgg aggtgaaggg cgcgtcgagc agctcggtca
     3721 gcggcagcgc gtcgatgtcc gcgcgccacg cgatggtcgg ccccgggcgg tcgccgtgga
     3781 gcaaacccac cacgccggtg ccgccgacgc cggtccggac gtccagtttc gccgcgtgca
     3841 gacgctccgc gatgatctcc gcggtgcgcc gctcctgatg gctcagctcc ggatggcgat
     3901 ggaagtcgcg acggagcgcg accagatcgt ccgcgcgctt ggtgatctcc tggctccatt
     3961 ccgggcgtgt cttgctcatt cgggcgtctc ctcgggcggt aattttttgc gcggccccga
     4021 tgctgcgccg tggccggccc gctgtcaacc ggcccgcacc cgccacgacc tgccaagatt
     4081 gagccgcgcg gtgaccacgg cgggaacctg cccggcggcc cctcccggcc aacccctcga
     4141 tttgcgcggc ttttcggcga tgcgtggctg gcccgtccct tgctcctctc cagacgatca
     4201 tgagcctcgc ccctcgcagc agccgccgtc tccacgtccg caagaacgta tcgtggaccg
     4261 cgtgggtcac ggtggggaag acccgcatcc gctgccacac cgtggacctg agcgccaatg
     4321 gagcccggct caagccgcgc ggggacttcc agcccgggac ccccgtccag ctgcagttcc
     4381 agccgccgga cgggccgatg ataggggtcg ccgcggtcgt gtggcgcgtg gaagcggaga
     4441 gcatggccgt catgttcctc cgcaacatcg ccgtacaggt ggcggtgccc agtcgcatgc
     4501 cggagcacgg ccgccgcggc tggcgctagt gcccgtcttc cccggcccgc gctcgtcgct
     4561 tgaccggccg ctgcgcgcgc gcgagtatcc ggcagcaact ccccgtcact gacgggtgag
     4621 cccggctcca tgagaacatg ggtgggtatc tccccatgac caccaggact ctaaccgacg
     4681 ccctcgccac gctgctggcc gtgaccctcc tgatcggctg cggtagcgac tacccggcgc
     4741 cgggcgccgc gaaatccgcc ccctcgggga ccgcgggcgc cgcgatcccc gccaagagcc
     4801 catcggtccg ggtcgtcccg gcgatggaag agcgcgcggc gggtgtcgtg gtggcctcgg
     4861 gcaccctcgc ggccgaagag caggtcgtgc tgggaatgaa ggtcgccggc cggctcgccg
     4921 agatcaccgt ggacctgggc acccgcgtca agcggggcga cgtggtggcg cgcctcgatc
     4981 ccaccgactt ccggctccgg gttcagctcg cggagaacgc catccagcag gcgcgggcgc
     5041 ggctcggcct gcctcaggac ggcgcgagcg accgcgtgga tccggacaag actccgctgg
     5101 tccgccaggc gcaggcggtg ctcgacgagg cgctgctcac ccgcgatcgg cggatcaagc
     5161 tgtgggagca gcagttcatc gcgcgggccg agctcgacgc cgccgaggcg gcggccaagg
     5221 tcgcggagag ccggtaccac gacgcggtcg aggaggtgaa caatcgcctg ggtgtcctcg
     5281 ccgagaagcg caccgagctc gagctggccc ggcagcagct cgcggacacc gtgctgcggg
     5341 cgccgatcga cggcgccatg aacgcgcgcc aggccgcggt gggcgagttc ctgaacgcgg
     5401 gggcgccggt agcgacgctg gtgcggatcc atccgctgcg gctccggctc gcggtgcccg
     5461 agcgcgaggc gaccgccatc cgccagggcc agcgcgtgcg cctgacgctg gagagcgatc
     5521 tgacggtgtc cgagggccac gtggcgcgcc tggcccccgc gatcagcgag cagaaccgct
     5581 ccctcatgat cgaggccgag atccccaacg agcagggccg gttgcggccg ggggcgttcg
     5641 cccgcggaga gatcgtcgtg cagccggacc ggaagctcgt caccgtgccc gcggaggcca
     5701 tcgtgacctt cgcgggggtc gagaaggtga tggtggtcga ggccgggcgc gcggtcgaga
     5761 agcgcgtgac caccggccgt cgcaccgggg atcgcgtcga gattctctcc ggcctcgccg
     5821 cgggggagca ggtcatcgtc cgcccgggcg ccgtcgcctc cggacagccg gtcaccgcga
     5881 gcccctgacg tgcagaagct cgccgaggtt tgcatccgcc gccccgtctt cgccgcgatg
     5941 ctcatcctcg cgctctgcgt ggtcggcgcg gccagctact tccgcctcgg ggtggaccgc
     6001 ttccccgccg tcgatctgcc caccgtcacc gtccgcacca ccctggccgg cgcctccacc
     6061 gaggagatgg agatccagat ctcccagcga atcgaggagg tcgtcaacac cgtggcgggg
     6121 atcagcgagc tgcgctcgat ctccgggccg ggcagctcgg tggtgatcgt cacgttcgag
     6181 ctctcccgga acatcgacgt ggccacccag gacgttcggg acaaggtcgc taccgtagtc
     6241 cgcaacctcc cgcgcgacac cctcgctccc atcatctcca agtccgacaa cgagtcttcg
     6301 ccggtgatga cggtggcgct ggccggcgac cggccgctac gggagctgac cgagctggcg
     6361 gacaaggtgg tgaaggtccg gctcgagcgc tcctccgggg tgggcgaggt gcgcctcgtg
     6421 ggcggcctcg agcgggccat cagcgtgtgg atcgacgcgg accgtctggc cgcctaccag
     6481 atccccatca gcgcggtgcg cgcggcaatc gttcgccaga acgcggacct tcccggcggc
     6541 aacgtcaccg agggcgcgca cgagtccgcg ctgcgcacga tggggcgggt gatggatccg
     6601 aaggccttcg acgacctggt catcgccacc atcaacgggg tggcggtccg ggtgcgcgac
     6661 atcggccacg ccgaggacgg gaccaaggag cagcgttcga tcgcgcgcct cgacggcgtt
     6721 cccaccgtga ccctcgaggt gcgccggcag tcgggcgcca acacgatcgc ggtcatcgag
     6781 gcggccaagg ccaacctggc ccggatcgcc gggcagctcc ctcccggtgt ccggctcgag
     6841 gtgatccggg accagtcgcg ctacatctac gcggcgctgc gcgagatcaa cgtgcatctc
     6901 gtcctgggcg ccatcctcgc ctcgctggtg gtcctggcct tcatgcggag ctggagctcg
     6961 accctcatcg cggcggtggc catccccgcc tcggtgatct ccgccttcgg catgatgtgg
     7021 gcgctcaact tcacgctcaa cagtgtcacc atgctcgccc tcgtgctcat ggtgggcatc
     7081 gtcatcgacg acgcgatcgt cgtgctcgag aacatcttcc gcttcgtcga ggagaagcgg
     7141 atggagccgt tcgcggcggc ccgcgcggcc accgccgaca tcggcctcgc cgtgctcgcc
     7201 accaccttga gcctggtcgt gatcttcgtg cccgtctcct tcatgtcgtc gatctcgggc
     7261 cgcttcctgt accagttcgg actcaccgcc gcggtggcgg tcctggtgtc tctgctcgta
     7321 tcgttcaccc tcactccgat gatgagcgcc cggctcctgc gcgccgaggc ccgcaagcgc
     7381 gggccggcgg gcgagggcga gacgcacgtc gcggtgtcgc gccgcggctt ctacgcctgg
     7441 atcgaccgag gctacgcgtg ggcgctcggg ctctccatgc gccatcgcat cgcggtggcc
     7501 ctgatcgccg ccctggtgat cgcggcgacg gtgcccctgt atgggctcgt gcggcaggag
     7561 tacatcccga ccgatgtgga cgaggcggag ttcgacgtcc gggtcaccgc cccccagaac
     7621 gtgagcgtga ccgccatgga cgaggtcatg cagcgggtag cccaggagat caccgtcgcc
     7681 cccggggtgc ggctcgtgct cgcctcggcg ggcggcggct tcctgggcca ggtcaacgag
     7741 ggcaccgtct acgtgcgcct ggccccgcac ggggaacgca ccgtcacgtt cgcgcggatc
     7801 tggcgcgggg tgatctccgg ggacccgctc gcgggcttcc ggggcctctg gggccagcgc
     7861 gacgtgatgc aggaggtccg ccggcggctc gcccgcatcc ccgacctgcg cacctcggtg
     7921 cggaacgctc cctcgttcaa cacgggaggc ggcagcttcg acatcgactt catcatccgg
     7981 ggcccggacc tgaacgcgct ggcgcggtac ggtgaggccc tgcgggagcg ggcccgccag
     8041 ctcggcggca tcgccgaccc cgacaccacg ctcaagctcg ataagccgga gctgcgcgtc
     8101 gagatcgacc gggcgcgggc cgccgatctc ggcgtggaca ccgagcaggt cgcggagacc
     8161 ctccgcctca tggtgggcgg cgatcaggaa gtgtcgcgct tcctagatcc gaccgtgaac
     8221 gacgactacg atgtgcagct ccgggtcgcc gcgggcgacc ggcgcgaccc gggagcgatc
     8281 gcgcgtttct acgtgccgcg ccagaacggg ggcggcctcg tgcggctgga cagcgtggtg
     8341 aagatcgtcc ccgcggaggc ggcgtcccgt atcgaccgct cggaccgcca gcgcgagaac
     8401 cgtctccggg cctcagtcct gcccggcttc ggccaggccg atcggatcca ggcgctccgg
     8461 agcgcggtcg ccgagatgaa cctgccgacc gcctacacca ccgcgatcgc ggggcgcgcc
     8521 cgtgagctcg agcgcacctt cgtggagttc ctgtgggtgt tcctgctctc ggtgatcttc
     8581 atgtacatga tcctggcctc gcagttcgag agcctcgtcg atcccctgac catcctgatc
     8641 tccctgccca tctcggtgcc cttcgcgctc ctgtccctct ggctcaccag ccacacgctc
     8701 aatctctact cggccctcgg catcctcgtg ctcttcgggg tggtcaagaa gaactcgatt
     8761 ctccagatcg accacatgaa cacgctgcgc gcccacggca tggaacgcct cgccgccatc
     8821 atgcaggcca accgcgaccg tctgcggccg atcttgatga ccacgctcgc gctggtggcc
     8881 ggcatgttac cgctggccgt cggctcgggc ccgggggcgg aggagcgccg ggccatcgcg
     8941 gtcgtggtga tcggcgggca gaccctctcg ctgctgctga ccctcctggt gaccccggtc
     9001 gcctactcgc tcttcgagga cctcgcccgg atgcttggct ggcagagcct cgtccgcatc
     9061 ggccgccgcg gcctccgggc cgcgcgagcc cgccgctaga cgggtcgggc ggggcccgcg
     9121 cgccccgcct cgggcccttt gacagcgcgg cgcccttcgc ctagactccc cggcgtatcg
     9181 atccggctcc ggggagacct catgggcttc gtctgctcga gttcgctcgc ccgatcgtcc
     9241 tcgtctcccc ctgcctgctc cacccacacc cgccaccagt cccgcgcgtg atcaagtgag
     9301 gaagccatga acaagctcat cgtagccgcc ctggtgctgc tagcggccgt cgcccaggtc
     9361 tcggtcgcgg cggcccagat cagcgcgcct gcgccgaccg caccgaattc ccagggcctg
     9421 ccgtggtggt tcgtcaagca ccagtacacg ccgggtatgc cgtggttcta ccgagccccc
     9481 ggcgcgcctg ccgcgaggca gtgccgggtc gcgtactggt acccggcgca gttctggaac
     9541 acggaccagt ggggcaacac ggtgacgtgg acgagctacg cgccccagta cgtctgcgac
     9601 tgacgaccgg aagttctgtc gcccgccctc cggatctctg ctagcgtgcc tcccgtgggc
     9661 gcgcaggcga cgtggcgcga ggtgatcccg gaaggcgaag ccgcgcgcct cgaagcgctg
     9721 gcgaagcgcc tcctcggccg ccgcaggctc gacggccggc cgcttcaccg caagacgctg
     9781 gccgcgctcc acgcgcgctt cgaggtcgcc gaggcgccgc gcgatctccg ccacggactc
     9841 ttcgccgagc cggggagcta cgacaccttc gtgcgcttct cctcgggcgc gggccggtca
     9901 cggagcgacc gcgtgccgga cgcgcgcggc ctcgcggtca agatcctggg cgtcaccggc
     9961 ccgaaggtga tccccggcct cgagacggcg tccacgcagg acttcctcgc gatcctctcc
    10021 cccaccttcc cgttcaagga tccggccgcc ttcgtggaca tcgcggtcgc gtcacggcga
    10081 ggcaaggtcg cggtggtgcg cgcggcgctc aggcactacg gcccgctcgg cctgttcggg
    10141 gcgcttcccc gactgcagcg cgcgctggac tcctccatgc ggagcctggc cgagcgcacc
    10201 tacttcaccg cggtcccgct gcgcctcggt ccccatgcgg tcaagctgcg cttccgcccg
    10261 atggatgtgc ccccgccggt gccgccggtg ggcggtccgg acgcgctcgc ggtcgagctc
    10321 caggcccgcg tgcgggcatc gccgctgtcc ttccgcgtgg aggcgcagcg cttcgtggac
    10381 gagctacgca ccccgatcga ggatccgacc gtggagtgga ccgagcggga cgcgccgtgg
    10441 gtggcgctgg cccggctggc gatccccgcg caggcggccg agtcacgcgc tggccgcgcg
    10501 ctggccgcct acgtggagcg cctgtccttc gatccgtggc acgccctggt cgagcaccgc
    10561 ccgctcggcg tggtcatgcg ggcgcgggcg gtcgcctaca agcgcgcggt catcgcccgc
    10621 ggcgccatct ccgagctcga ggtcaagccg ccatccgccg tgatgggctg acgcgcggga
    10681 ggcctacacc cgcactccgg ccttcaccct ggccggggtg aacggcacgc tccggacgcg
    10741 caccccgacc gcatcgaaga tggcgttgga gatcgccggc ggcggcaggc acgcggcggg
    10801 ctctcccacg ccccacggcg gctccccggg cctattgatc agctccatca cgacctcggg
    10861 cacctccggg aacctgagga tcgggtaact cgcccagtcc actgaggtca cccgcgagcg
    10921 gtcccacgtg agctcctcct tgagcgtccg cgagacggtc tggatgatat tgccctcgat
    10981 ctgcgcgcgc accccgtcgg gattgacaat ctgcccgcag tcgtgggcga cgaagaagcg
    11041 cgtgcagcgc accgcgccgg tctgccggtt gacttcgacc tccgcgaccc cggcgacgta
    11101 ggtgcgggcg ttctcgtact tcacgtacgc gatgccccgg ccccgcgcca ccgggccgga
    11161 cgcggccttc gccggggagg gccgggtgtc ccacttggcg agatgcgccg ccgcgcgcag
    11221 caccgcgatc ccccgcgggt cggtcaggta acgcagccgg tactcgaccg gatccgcgcc
    11281 ggccgccgcc gcgcactcgt cgatgaacac ctcgttcgca taggtgttct gcatgcggcc
    11341 tggggtgcgg atccacgagg gccggaacgg ggtggtctcg agccggtgcg ccaccgcctt
    11401 ctggccggga cggtgtcaag gcctggtccc cgggcggcgc gggtcgcggc tatactacgg
    11461 gccagcgagt caccactcga gaaggagacc acgatgaacg gagcggagag tctggcgcga
    11521 acgctggtgg cgggtggggt caacgtctgc ttcacgaatc cgggcacctc cgagatgcac
    11581 ttcgtggccg cgctcgacaa ggtggacggc atccgctgcg tgctcggcct cttcgagggc
    11641 gtggtgacgg gcgcggccga cggctactgg cgcatggccg acaagccggc cgccaccctg
    11701 ctgcacctgg gccccgggct cggcaacggg ctcgccaacc tccacaacgc gcgcaaggcc
    11761 agctcgggca tcgtgaacat cgtcggcgag cacgcgacgt accacgtcaa gcacgacgcg
    11821 ccgctcacct ccgacatcga gggcatcgcg cgcccggtct ccgcgtgggt gcgcaccgcg
    11881 ccgagcgccc gcgcggtggc cgccgacggc gcggcggcga tcgcggcggc gcgcaccgcg
    11941 cccggccagg tcgccaccct gatcctgccc gccgacaccg cgtggaacga ggccgacggg
    12001 atcgccgcgg tgcccccggt ggccgcgcgg gcgtccgtcg gcacggaagc ggtcgagcgc
    12061 tgcgccaagg cgctgcgctc gggcgagccc gccatgctca tgctgaccgg cgtcgcggtg
    12121 cgcgagcgcg gcctcgcgct ggccgggcgc atcgccaccg cgaccggggc gcgcatcatc
    12181 gcccagggct cgaacgcgcg catccagcgc ggcgccggcc gcgtgtcgat cgagcggctg
    12241 ccgtatccgg tggaccaggc gctggccgtg ctgaaggacg tgcggcacct catcctggtc
    12301 ggcgccaagg ccccggtggc cttcttcgcg tatccggaca agcccagcgt gctcacgccg
    12361 gatggctgcc aggtgcaccg gctctcgacg ctggccgagg attcgatggg cgcgctggaa
    12421 gcgctggccg aggcagtggg ggccaagaag aacggcgagg tggtccagca gcccggtcgc
    12481 cccgcgctgc ctaccggcgc gctcaccccc gacacgctgg ccgccgcgct cggcgcgctc
    12541 ctgcccgcgg gcgccatcgt ctccgacgag tcggtaacca ccggccgcgg cttcttcaag
    12601 atgaccgcgg gcgccccgcc tcacgactgg atgaacaaca tgggcggctc gatcggcctc
    12661 ggcctgccgc tcgccaccgg ggccgcggtg gcgtgccccg accgcaaggt cgtgtgcctc
//
LOCUS       DASUFA010000197         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1415554, whole genome shotgun sequence.
ACCESSION   DASUFA010000197 DASUFA010000000
VERSION     DASUFA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415554"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1554)
                     /locus_tag="VFX87_07125"
     CDS             complement(31..1554)
                     /locus_tag="VFX87_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4994721.1"
                     /translation="MTSSAVAATAGTPGGDAARRDPLAIPPEVKASIRVLVVDDERTL
                     RESCASVLQMDGYNVTVIGRGEEALEAVRRRRFDIVLVDLYMSQVSGMDILQAALQAA
                     KDTIVVVMTGNPSVATSIEALRAGAWDYLPKPFSATHLQVLVGRASHAVMVARETNDL
                     RLQVMRQSGQGEKFALIGIAAPFREAVELARKVAGTDASVMITGESGTGKEQIAQFIH
                     HHSRRSSRRLVAVNCAALPEPLLESEMFGHRKGAFTGADRDKPGLLETANGGTLLLDE
                     LTEMTPPLQAKLLRVIQDGVVRRVGSEQPDAVVDVRFISATNRDPQEAVAQGVLREDL
                     YYRLRVVPIKLPPLRKRPEDIPILANHFLAMFWLRHRQMGDRMPHFSDEAVAFLRSRP
                     WRGNVRELQNVIEHVAVLCEPEQLIEPKDIPLYDEPAAEPHAGVGLPEGLYEEAYHLA
                     KDRVVAQFEKEYLGRVIGRANGNMSKAARLASIDRTTLYRLLDKHGLQRDELSGPAE"
     gene            1909..4308
                     /locus_tag="VFX87_07130"
     CDS             1909..4308
                     /locus_tag="VFX87_07130"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX4994722.1"
                     /translation="MAGELVPTVGQSDPRSLPVPGVMGPQSGFPQAPPVEEEQGIQWG
                     RYIAALRRYRWLILGVTVLGTALAVFATRFIKPQYTAAATIWIEPRRDEGAAPIRAEQ
                     LLQQTFAWVELLRTPVVLDSAVLKMKLYLEPRDAKDSTVFRGFGLLSRFYPGRYVLAM
                     AEDGRSWELRMKSGGLAPERGAVGDSVGRRFGFAWLPPRERLGKGREIEFIVRHPRDA
                     SMQLGTDLITQTADDGNFLRVALTGTDPHRISNTLNAVTDQFVALAADLKTRKVVDLM
                     GVLEKQVEYAADNVAKTESALENFRTRTITQPSETPIVSGLAATQNPVLSNYFERKLQ
                     AEAMREDREALESVLRRAQSGDFSVDAFQTVASVQGAPDLKLALAEVSSAEAELRALR
                     FRYTDQHKPVQDLTARVNQLKNEIVPQYATALIGQLRTQERDLSQSIATRTRELQSIP
                     TRTITEQKLTRDAMSAERLYLELQSRYEEAKLAAASAQPDVQVLSEAVPPTQPAKNEA
                     IRLILMGFLASLGAAVALAILLDQIDKRFRYPEQITNELGLAILGAVPTINKTKVNER
                     DPEEAAQIIEAFRTIRLNLAHSYGAAGPVLLTISSPSPGDGKSLIASNLALSFAEAGY
                     STVLVDGDIRRGELHRMFNLSRRPGLMDCLSGDATLEDVLQPTSHQGLTVMPCGSRRH
                     QGPELLGSAAMQAVMAQLKGRFQVVLVDSPPLSAGIDPFVLSASTGNMMVVLRSGETD
                     RTLAEEKLKLMDRLPVRVLGAVINSTSATGAYRYYHYVYGYTTEDEGASARLPAGAGS
                     A"
     gene            complement(4390..>4909)
                     /locus_tag="VFX87_07135"
     CDS             complement(4390..>4909)
                     /locus_tag="VFX87_07135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4994723.1"
                     /translation="VRDEALLDALTGLYNRRGLTRRAREIATEAFRRRHALACVVLAT
                     ESVGAADAASGDRLLAEVGETLRRAGRVSDAIGRLGPNEFGVIAPATDAEGAMRMAER
                     IGAALRETRVSSDPDVAPMRVRAGYTAVPDYHEADVDPLEMLLRATTALRTAQPEGAH
                     HWLQAFEAAAAN"
BASE COUNT          697 a         1766 c         1732 g          714 t
ORIGIN      
        1 ccggcgtggc gcgggccggg ccggagtcgc tcactccgcc ggccccgaca gctcgtcgcg
       61 ctggaggccg tgcttgtcga gcaggcgata gagcgtggtg cgatcgatgc tggccaggcg
      121 cgccgccttg gacatgttgc cgttcgcccg gccgatgacc cggccgaggt actccttctc
      181 gaactgcgcc accacgcggt ccttggccag gtggtaggcc tcctcgtaga gcccctccgg
      241 cagccccacc ccggcgtgcg gctcggccgc cggctcgtcg tagagcggga tgtccttggg
      301 ctcgatgagc tgttccggct cgcacaggac cgccacgtgc tcgatcacgt tctgcagctc
      361 gcgcacgttg ccgcgccacg gccgggagcg gaggaaggcg acggcctcgt cgctgaagtg
      421 cggcatccgg tcgcccatct ggcggtggcg gagccagaac atggccagga agtggttggc
      481 gaggatcggg atgtcctcgg gccgcttgcg gagcggcggg agcttgatcg gcaccacccg
      541 caggcggtag tacaggtcct cgcggaggac gccctgcgcc acggcctcct ggggatcgcg
      601 gttcgtggcc gagatgaagc gcacgtccac cacggcgtcg ggctgctcgc tgcccacccg
      661 gcgtaccacg ccgtcctgga tgacgcgcag cagcttcgcc tggagcggcg gcgtcatctc
      721 ggtgagctcg tcgagcagga gcgtgccgcc gttcgcggtc tcgagcaggc cgggcttgtc
      781 gcggtcggcg ccggtgaagg cccccttccg gtggccgaac atctccgact cgaggagcgg
      841 ctcgggcagc gccgcgcagt tgaccgccac gaggcgccgg ctggaccgcc ggctgtggtg
      901 gtggatgaac tgcgcgatct gttccttgcc ggtgccgctc tcgcccgtga tcatcaccga
      961 cgcgtcggtg ccggccacct tgcgggccag ctcgacggcc tcgcggaacg gtgcggcgat
     1021 gccgatcagg gcgaacttct cgccctggcc gctctggcgc atcacctgga ggcggaggtc
     1081 gttggtctcg cgcgcgacca tcacggcgtg cgaggcgcgg cccacgagca cctggaggtg
     1141 ggtggcggag aacggcttgg gcaggtagtc ccaggcgccc gcgcgcaggg cctcgatgct
     1201 cgtcgccacg ctcgggttcc cggtcatgac gacgacgatg gtgtccttgg cggcctgcag
     1261 cgccgcctgc aggatgtcca tcccggagac ctggctcatg tacaggtcca ccaggacgat
     1321 gtcgaaccgg cgccggcgca ccgcctcgag ggcctcctcg ccccggccga tcaccgtcac
     1381 gttgtacccg tccatctgga gcacgctggc gcagctctcc cggagcgtgc gctcgtcgtc
     1441 caccaccagg acgcggatgc tcgccttcac ctcgggcggg atcgccaggg ggtcgcgacg
     1501 cgccgcgtcg ccccccggag tccccgccgt cgcggccacc gccgaactcg tcatccgtgc
     1561 cttccggttc tggactggat catccgtttg acctcgtggc cgggcgcgtg gtcacaactc
     1621 cacacccgtg acgccgacgc atcattgcag gacggcaaga tattgcattt tgcgacactt
     1681 ttccatcaca attgcgttgc acgatggcaa caccaccgca acactattgc ctcaggagca
     1741 tcgatggcgc aacactttag ccatgaccgc catccggcgg cccccacagt gggaaggaag
     1801 ttcggtgccg cgctgtgacc ggcgtgacgg taccgccctt gcgtgggtcg ttctgttgtt
     1861 cgggcggccc ggatggccag cccctgtctc atcctgtcga gagtgctgat ggccggagaa
     1921 ctcgtaccca ccgtcgggca gtcggacccc cggagcctcc cggtgcccgg cgtcatgggc
     1981 ccccagagcg gctttccgca ggccccgccg gtcgaggagg agcaggggat ccagtggggg
     2041 cgctacatcg cggcgctccg gcgctaccgc tggctcattc tcggcgtcac ggtcctgggg
     2101 acggcgctcg cggtcttcgc cacccggttc atcaagccgc agtacaccgc cgcggccacc
     2161 atctggatcg agccccggcg ggacgagggc gccgcgccga tccgggccga gcagctgctc
     2221 cagcagacct tcgcctgggt ggagctgctc cggacgccgg tggtgctcga ctcggccgtg
     2281 ctcaagatga agctctacct ggagccgagg gacgcgaagg actcgaccgt cttccgcggc
     2341 ttcggcctgc tcagccgctt ctaccccggc cggtacgtgc tggcgatggc ggaggacgga
     2401 cgttcctggg agctgcggat gaagtcgggc ggcctcgcgc cggagcgtgg ggcggtcggc
     2461 gactcggtgg gccgccgctt cgggttcgcc tggctcccgc cgcgcgagcg cctgggcaag
     2521 ggccgcgaga tcgagttcat cgtccgccat ccgcgcgacg cctcgatgca gctcggcacc
     2581 gacctcatca cccagacggc ggacgacggc aacttcctcc gggtggcgct caccggtacc
     2641 gatccgcacc ggatctccaa caccctgaac gccgtcaccg accagttcgt cgcgctggcc
     2701 gccgacctca agacccggaa ggtcgtggac ctgatggggg tgctggagaa gcaggtggag
     2761 tacgccgcgg acaacgtcgc gaagaccgag tcggcgctgg agaacttccg gacccgcacc
     2821 atcacccagc cgtcggagac gccgatcgtc tccgggttgg ccgccacgca gaacccggtg
     2881 ctctcgaact acttcgagcg gaagctccag gccgaggcga tgcgggagga ccgggaggcg
     2941 ctggagtcgg tgctgcgccg ggcgcagagc ggggacttct cggtggacgc gttccagacc
     3001 gtggcgtcgg tccagggtgc ccccgacctc aagctcgccc tggccgaggt ctcgagcgcc
     3061 gaagccgagc tccgcgcgct ccgcttccgc tacaccgacc agcacaagcc ggtgcaggac
     3121 ctcaccgccc gggtgaacca gctcaagaac gagatcgtgc cgcagtacgc caccgcgctg
     3181 atcggccagc tccggacgca ggagcgggac ctcagccaga gcatcgccac ccggacccgg
     3241 gagctccagt cgatcccgac gcggacgatc acggagcaga agctcacccg cgacgcgatg
     3301 agcgccgagc ggctgtacct cgagctccag tcgcggtacg aggaggccaa gctggccgcg
     3361 gcgagcgccc agccggacgt gcaggtgctc agcgaggcgg tgccgcccac ccagcccgcg
     3421 aagaacgagg ccattcgcct catcctgatg ggcttcctcg cgagcctggg cgcggccgtg
     3481 gccctggcga tcctgctcga ccagatcgac aagcgcttcc gctaccccga gcagatcacc
     3541 aacgagctgg ggctcgccat cctcggcgcg gtgccgacga tcaacaagac caaggtgaac
     3601 gagcgcgacc cggaggaggc cgcgcagatc atcgaggcct tccggaccat ccggctcaac
     3661 ctggcccact cctacggggc ggcggggccg gtgctgctca cgatctccag tccgagcccg
     3721 ggcgacggca agtcgctcat cgcctcgaac ctcgcgctct ccttcgccga ggcggggtac
     3781 tcgaccgtgc tggtggacgg cgacatccgg cggggcgagc tccaccggat gttcaacctg
     3841 agccggcgcc ccgggctgat ggactgcctg tccggcgacg cgacgctgga ggacgtcctc
     3901 cagcccacga gccaccaggg cctcaccgtc atgccctgcg gcagccgccg gcaccagggc
     3961 ccggagctgc tgggctcggc cgcgatgcag gccgtgatgg cgcagctcaa ggggcgcttc
     4021 caggtcgtcc tggtggacag cccgccgctg tcggccggca tcgacccgtt cgtcctgagc
     4081 gcgtccaccg gcaacatgat ggtcgtgctg cgctccggcg agaccgaccg gacgctggcg
     4141 gaggagaagc tcaagctgat ggaccggctg ccggtgcggg tgctcggcgc ggtcatcaac
     4201 agcacctcgg ccaccggcgc gtaccgttac taccactacg tgtacgggta caccacggag
     4261 gacgaggggg cgtcggcgcg gctcccggcc ggcgccggga gcgcctgacc gggcgggagc
     4321 ggcatgaacg gcgaggggcc cgccggcggc tgccggcggg ccccagttgt ccgcgcgtcg
     4381 ttcgcgtcct cagttcgccg cggccgcctc gaaggcctgg agccagtggt gggcgccttc
     4441 cggctgcgcg gtgcggagcg cggtggtggc gcggaggagc atctcgagcg ggtccacgtc
     4501 ggcctcgtgg tagtccggga ccgcggtata gccggcccgc acgcgcatcg gggccacgtc
     4561 gggatcggac gacacccggg tctcccggag ggcggcgccg atccgctccg ccatccgcat
     4621 cgccccttcg gcgtcggtgg cgggcgcgat cacgccgaac tcgttcggcc cgagccgccc
     4681 gatcgcgtcg gacacgcggc cggcgcgtcg cagcgtctcg cccacctcgg cgagcaaccg
     4741 gtcgccgctg gcggcgtccg ccgcacccac ggactcggtc gccagcacca cgcaggcgag
     4801 ggcgtgccga cggcggaacg cctcggtggc gatctcgcgc gcgcggcggg tcagcccccg
     4861 ccgattgtag agcccggtca gcgcgtcgag cagggcttcg tcccgcacc
//
LOCUS       DASUFA010000198         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1170879, whole genome shotgun sequence.
ACCESSION   DASUFA010000198 DASUFA010000000
VERSION     DASUFA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170879"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFX87_07140"
     CDS             <1..384
                     /locus_tag="VFX87_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994724.1"
                     /translation="LGAFTMASIARLTRSAVLEVLGADYIRTARAKGVAEVWVVAKHT
                     LKNAAIPIVTITGLQFGTLLGGAVVTETVFAWPGIGRLAVQSIYNRDYAVVQCTVFLA
                     ALMFVVINFFVDLIYGVLDPRIRAG"
     gene            350..1258
                     /locus_tag="VFX87_07145"
     CDS             350..1258
                     /locus_tag="VFX87_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994725.1"
                     /translation="MASSIRASAPADAALAAPAARVPARRRRFPALVVAGVGFVAVLL
                     VAAAAAPLLAPQDPIRQSLRGRLAAPTWQGSDGHAHLLGTDHLGRDVLSRVIYGSRVS
                     LVIGFSAVLVGGLLGSAAGLGAGFSRGRMDAAIMTIADAQLAFPFVLLAIGIIAVLGP
                     SFPTLVVVIGLSGWVSYARILRSQVLVLRSREFVESIHALGGSLARVVLRHVLPNVLS
                     SIVVIATLELARAIVLEATLSFLGLGIQPPTPSWGGMVQEGREYLDSAWWISTFPGIV
                     LMITSIVVSRTGDWLRDVLDPTLRGE"
     gene            1278..1691
                     /locus_tag="VFX87_07150"
     CDS             1278..1691
                     /locus_tag="VFX87_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4994726.1"
                     /translation="MPITELIEYEFASPEVREVYDDIMATRKTDWINNFWKALANHPP
                     TLRRTWESIKQIMGPGALDPLTKEMIYLAVSAANGCDYCIASHTAGARRAGMTEAMRG
                     ELLAVVGMACETNSLANAYQVPVDVRFRDPDPDTD"
     gene            complement(1640..2569)
                     /locus_tag="VFX87_07155"
     CDS             complement(1640..2569)
                     /locus_tag="VFX87_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4994727.1"
                     /translation="MGAERSGRLLVAAAAVCWSTGGLIARLVDTDPWTTVFWRGLFCV
                     AFLVAVTALRERRRAPDAFLGMGRPGLAMAVCFATASTCFIMALHLTSVANVLIIQSL
                     SPFMAGLLGWLWMGERVGGRTWAAMGVALLGSAIMVSRYFYTEAAAGSIGGDLLAFTV
                     ALAFAMATVILRRNRHVRMLPAAALAAALTSVVTAVSARPGTAGAGDLLLLALFGSGQ
                     LGLGMILFTAGARRIPVAEAALIAVLESVLGPVWVWLAIGENPGVPSLAGGAIVLAAL
                     AGHTLADLRQEWRAEAAQSVSGSGSRKRTSTGT"
     gene            complement(2583..>2781)
                     /locus_tag="VFX87_07160"
     CDS             complement(2583..>2781)
                     /locus_tag="VFX87_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994728.1"
                     /translation="GRTGDHRPEGLFVAAGPGLGPARLSRSVSVMDFAPTLAALLDVE
                     VPSPDGQPIHELLAARPRAGA"
BASE COUNT          374 a         1030 c          995 g          382 t
ORIGIN      
        1 ctgggcgcgt tcaccatggc ctccatcgcc cggctcaccc gctcggcggt gctggaggtg
       61 ctgggcgcgg actacatccg caccgcgcgg gccaagggcg tggcggaggt gtgggtggtg
      121 gccaagcaca cgctcaagaa cgcggcgatc ccgatcgtga ccatcaccgg gctgcagttc
      181 ggcacgctgc tgggcggcgc ggtcgtcacc gagaccgtct tcgcgtggcc cggcatcggc
      241 cggctcgccg tgcagtccat ctacaaccgc gactacgcgg tggtgcagtg cacggtgttc
      301 ctggccgcgc tcatgttcgt ggtgatcaac ttcttcgtgg acctgatcta tggcgtcctc
      361 gatccgcgca tccgcgccgg ctgacgcggc cctcgccgcg cccgcggccc gggttcccgc
      421 gcgccggcgc cggttccccg cgctggtggt ggcgggcgtg ggcttcgtcg cggtgctgct
      481 ggtggcggcc gccgccgccc cgctcctcgc gccccaggat ccgatccgcc agagcctgcg
      541 cggccgtctc gccgcgccga cgtggcaggg ctccgacggg cacgcccatc tgctcggcac
      601 cgaccacctg ggccgcgacg tgctctcgcg ggtgatctac ggctcgcggg tctcgctggt
      661 gatcggtttc tccgcggtgc tggtgggcgg cctgctcggc tcggcggcgg ggctgggggc
      721 cggcttcagc cgcgggcgca tggacgcggc catcatgacg atcgcggacg cgcagctcgc
      781 cttcccgttc gttctgctcg ccatcgggat catcgcggtg ctcggcccga gctttcccac
      841 gctcgtggtg gtgatcgggc tctccgggtg ggtctcctac gcgcgcatcc tgcgctcgca
      901 ggtgctggtg ctgcgctcgc gcgagttcgt ggagtccatc catgccctgg gcggctcgct
      961 cgcccgcgtg gtgctccgcc acgtgctgcc caacgtgctc tcctcgatcg tggtcatcgc
     1021 caccctcgag ctggcgcggg ccatcgtgct cgaggccacg ctgtccttcc tcgggctggg
     1081 catccagccg cccaccccgt cctggggcgg catggtgcag gagggccgcg agtacctcga
     1141 ctcggcctgg tggatctcca ccttccccgg gatcgtgctc atgatcacct cgatcgtggt
     1201 cagccgcacc ggcgactggc tccgcgacgt gctggacccg actctccgcg gggagtaggg
     1261 cgaaaggaga tacttggatg ccgatcaccg agctgatcga gtacgagttc gccagccccg
     1321 aggtgcgcga ggtctacgac gacatcatgg ccacgcgcaa gacggactgg atcaacaact
     1381 tctggaaggc gctcgccaac cacccgccga ccctgcgccg cacctgggag agcatcaagc
     1441 agatcatggg gcccggcgcg ctcgacccgc tgaccaagga gatgatctac ctcgcggtga
     1501 gcgcggccaa cggctgcgac tactgcatcg cctcgcacac cgcgggcgcg cgccgggccg
     1561 gcatgaccga ggccatgcgc ggcgagctgc tcgcggtggt cgggatggcc tgcgagacca
     1621 actcgctggc caacgcctat caggtgccgg tggacgtgcg cttccgcgac cccgatcccg
     1681 acaccgactg agcggcctcc gcgcgccact cctgccgcag gtcggccagc gtgtggccgg
     1741 cgagcgcggc cagcacgatg gcgccgcccg ccagcgaggg cacgccggga ttctcgccga
     1801 tcgcgagcca gacccacacc gggcccagca cgctctccag caccgcgatc agggccgctt
     1861 ccgccaccgg gatgcggcgc gcgcccgcgg tgaacaggat catgccgagg ccgagctggc
     1921 cgctgccgaa gagggccagc agcagcagat cgccggcgcc ggcggtgccc ggccgcgccg
     1981 agaccgccgt caccacgctc gtgagcgcgg cggccagcgc ggccgcgggc agcatgcgca
     2041 cgtggcggtt gcgccgcagg atcacggtcg ccatcgcgaa ggcgagcgcc accgtgaagg
     2101 cgagaaggtc gccgccgatc gagcccgcgg ccgcctccgt gtagaagtag cgcgagacca
     2161 tgatggcgct gcccaggagc gccacgccca tcgcggccca cgtccggccg cccacgcgct
     2221 cgcccatcca gagccagccg agcaacccgg ccatgaacgg gctcaggctc tggatgatca
     2281 gcacgttcgc caccgaggtg aggtggagcg ccatgatgaa gcacgtggac gcggtggcga
     2341 agcagaccgc catcgcgagg ccgggccgcc ccatgcccag gaaggcgtcg ggcgcccggc
     2401 ggcgctcgcg cagcgcggtc accgcgacca ggaaggccac gcagaagagc ccgcgccaga
     2461 acacggtggt ccacggatcg gtgtccacga ggcgcgcgat cagcccgccc gtgctccagc
     2521 acaccgcggc ggccgcgacg aggagccggc cgctccgctc ggcgcccacg agcgcgggcc
     2581 gcctacgcgc ccgccctcgg gcgcgcggcc aggagctcgt ggatcggctg gccgtccggg
     2641 ctcgggacct ccacgtcgag tagcgcggcg agagtgggag cgaagtccat cacggaaacg
     2701 cttcgcgaga gccgggccgg cccgaggccc gggccggcgg cgacgaagag cccttcgggc
     2761 cggtggtcgc ccgtgcggcc g
//
LOCUS       DASUFA010000199         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_48281, whole genome shotgun sequence.
ACCESSION   DASUFA010000199 DASUFA010000000
VERSION     DASUFA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48281"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VFX87_07165"
     CDS             <1..1139
                     /locus_tag="VFX87_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994729.1"
                     /translation="VQNGAIARIAPQIAETEAARTIDVRGKVVTPGLIDLHAHVFEGV
                     NRTGVNPDLGGVYAGVTTIVDAGSAGSATFGGFPRHILPHCHTEVIPFLHICATGLAT
                     LPDIIAESSINLDDTLKVVDQNRHLIRGIKARMVSPALEIMGMEMPKLAKRAAKESGV
                     KLMVHIGDTEKRYDPKVIRSLLPILEKGDILTHYFTANPGGVLDSNGKLVPEVKEAAD
                     RGVWFDTAHGRMNFSFDVGKRVIEQGVLPHCISTDLTVPGRVMTVHSMTEMMTRFLGL
                     GFTLPQVVTMCTANPAKAAGVDDRLGSLAVGRQADISVLDIRPGDWMVYDVLGAGLRV
                     DRAVVPCVTVKKGRVFTPDWGPRSWGWEPDRALPAGGPIRGGCC"
     gene            1136..1786
                     /locus_tag="VFX87_07170"
     CDS             1136..1786
                     /locus_tag="VFX87_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX4994730.1"
                     /translation="MMPVVPEWSSLGLFMLAALVLLLTPGPAVLYIVSRSIDQGRRAG
                     LVSVLGVHVGTLAHIFAAAVGISAVLAASATAFSVVKYLGAAYLIFIGVQRLLDRAPL
                     GAATPGEPKRLRRALLDGVIVNVLNPKTGLFFLAFLPQFVTVSRGHVGEQIVVLGVVF
                     VLLGAITDSLYAIGAGTAAQWLRGRPRFLGSERWVSGGLYIGLGVAAALSSSQHRK"
     gene            2248..>2603
                     /locus_tag="VFX87_07175"
     CDS             2248..>2603
                     /locus_tag="VFX87_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994731.1"
                     /translation="MIAAVRAGMARVVGLPTRVRGLLPPLVAPAAQAAARVIGHVRFM
                     GPRGAHLLIRLRHGVARAAIEATGWVISAGRIGAVRILAARAGVARVVGLPTRVRGLL
                     PPLVAPAAQAAASVIG"
BASE COUNT          350 a          919 c          933 g          401 t
ORIGIN      
        1 cggtacagaa cggcgccatc gcccgcatcg ccccgcagat cgcggagacg gaagccgcgc
       61 gcaccatcga cgtccgcggc aaggtcgtca cccccgggct gatcgatctg cacgcccacg
      121 tcttcgaggg cgtcaaccgt accggggtga acccggacct gggcggcgtc tacgcgggcg
      181 tcaccaccat cgtggacgcg gggagcgccg ggtcggccac cttcgggggc ttcccgcgcc
      241 acatcctgcc ccactgccac accgaggtga tcccgttcct ccacatctgc gcgaccgggc
      301 tcgcgacgct gcccgacatc atcgcggaga gcagcatcaa cctggacgac acgctgaagg
      361 tggtggacca gaatcgccac ctgatccgcg ggatcaaggc gcgcatggtc tcgcccgcgc
      421 tcgagatcat gggcatggag atgccgaagc tggccaagcg ggcggcgaag gagagcggcg
      481 tcaagctcat ggtgcacatc ggcgacaccg agaagcgcta tgacccgaag gtgatccgct
      541 cactgctgcc gatcctcgag aagggtgaca tcctgaccca ctacttcacc gccaacccgg
      601 gcggcgtgct cgacagcaac ggcaagctgg tacccgaggt gaaggaggcg gccgaccgtg
      661 gcgtgtggtt cgacaccgcg cacgggcgca tgaacttcag cttcgacgtg ggcaagcgcg
      721 tgatcgagca gggcgtgctc ccgcactgca tcagcaccga tctcacggtg cccggccgcg
      781 tgatgaccgt gcacagcatg accgagatga tgacccgctt cctgggcctg ggcttcacgc
      841 tgccgcaggt ggtcacgatg tgtaccgcca atcccgcgaa ggcggcgggc gtggacgatc
      901 gcctcggcag cctcgcggtg ggccggcagg ccgacatctc ggtgctcgac atccgcccgg
      961 gcgactggat ggtctacgat gtcctgggcg ccgggctgcg ggtggaccgc gcggtggtcc
     1021 cgtgcgtgac cgtcaagaag gggcgcgtgt tcaccccaga ctggggcccg cgctcgtggg
     1081 gctgggagcc ggaccgggca ctgcccgcgg gcgggcccat ccggggaggg tgctgctgat
     1141 gccggtcgtg ccggaatggt cgagtctcgg gctcttcatg ctcgcggcgc tggtgctgct
     1201 gctgacgccc ggacccgcgg tcctctacat cgtcagccgc agcatcgatc agggccggcg
     1261 cgccggcctg gtctcggtgc tgggcgtgca cgtgggcacc ctggcccaca tcttcgcagc
     1321 ggccgtcggc atctccgcgg ttctcgccgc ctccgccacc gcgttcagcg tggtcaagta
     1381 cctgggcgcg gcctatctca tcttcatcgg cgtgcagcgc ctgctcgacc gcgccccgct
     1441 gggcgcggcc acgccgggcg agcccaagcg cctgcgccgc gcccttctcg acggcgtgat
     1501 cgtcaacgtc ctgaacccga agaccggcct cttcttcctg gctttcctgc cccagttcgt
     1561 gaccgtctcg cgcgggcacg tgggcgagca gatcgtggtg ctgggcgtgg tcttcgtgct
     1621 gctgggcgcg atcacggact cgctctacgc gatcggcgcg ggcaccgccg cgcagtggct
     1681 ccgcggccgc ccgcgcttcc tgggcagcga gcgctgggtc tcgggcggcc tctacatcgg
     1741 cctgggcgtg gcggcggcgc tgtcgagcag ccagcaccgg aagtagcggc gcccagggcg
     1801 ggaagctcag aatttcctgc ccgtacatcc ggccgcccgg cgtggtaagg tgcatgggct
     1861 acgccgactg acggagaggg ctgagcggcg aacaggaggg agggcaacca tgtgccctcg
     1921 gctggacctt gatcgccggc ggccgctcgc cacgttcccg gccttcccgg gccggatgac
     1981 gcgttgccca tgaattgcca tgaagtccgt gaggggctcg ctgcctcttc ccgcggcggg
     2041 atgggcctga cggagtgggc cctcgtgcac gcccatgtat cgcactgcgt ggactgccgg
     2101 aaggaacgag agtctctcca gccgggggtg agctcgcgtc agcgagtcgc actgtctcgc
     2161 ggggcctccg acttcgccgc ctcgctgacc cggctccgcg tgcgggcgtc gatctccctg
     2221 accgggtccg ggcaaacggc ggtgaggatg atcgcggccg tccgggcagg gatggcgcga
     2281 gtggtcggcc tgcccacccg ggtgcgcggc ctgctgccgc ccctcgttgc gccggcggct
     2341 caagccgccg cgcgcgtgat cgggcacgtc cgcttcatgg gcccgcgcgg tgcccacctg
     2401 ctgatccggc tccgccacgg ggtcgcgcga gccgcgatcg aggcgaccgg ttgggtgatc
     2461 tccgccggcc gcatcggagc cgtgcggatc cttgccgccc gggcaggggt ggcgcgagtg
     2521 gtcggcctgc ccacccgggt gcgcggcctg ctgccgcccc tcgttgcgcc ggcggctcaa
     2581 gccgccgcga gcgtgatcgg gca
//
LOCUS       DASUFA010000200         6455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1417021, whole genome shotgun sequence.
ACCESSION   DASUFA010000200 DASUFA010000000
VERSION     DASUFA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417021"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..868)
                     /locus_tag="VFX87_07180"
     CDS             complement(29..868)
                     /locus_tag="VFX87_07180"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX4994732.1"
                     /translation="MYERRRNIVVRRDGRRVNVARFRGQLFVCATGCCCGRTEDGFAP
                     VPTETFHREWERRRLRNIIHLTIGGCLGPCALANVALVLFDGEAQWFHSIDSDALAVA
                     LYDHVEAMLDADACLPPPPALAPYQFTASAWQSRPDGQPVDDARLWRGRRAHACESTP
                     SGMTAEGQDLTVDRVVAVMEGQAALPRKNGELVFEEPWQGRVFGMAVALHERGLYEWE
                     EFRQTLIAQIKAAEARSGPFVYYEIWLQTFEALLARKQLITDTELDETTYQFEFGERD
                     EVF"
     gene            complement(883..1479)
                     /gene="nthA"
                     /locus_tag="VFX87_07185"
     CDS             complement(883..1479)
                     /gene="nthA"
                     /locus_tag="VFX87_07185"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX4994733.1"
                     /translation="MGKNHPNVDPYIVSRVRALESLLLEKGILAPDAVDRVIQRYEAD
                     TGPMVGARAVAKAWTDPDYRRLLLDDSKAALARFDLDYEMLMVVENTDAVHNLVVCTL
                     CSCYPWPVLGLPPTWYKMPAYRSRAVSEPRTMLQEMGLAVPAEREIRVWDSSAEMRYM
                     VLPRRPAGTENMGEAELVPLITRDALIGVAEPRPTAAS"
     gene            complement(1485..2165)
                     /gene="nthB"
                     /locus_tag="VFX87_07190"
     CDS             complement(1485..2165)
                     /gene="nthB"
                     /locus_tag="VFX87_07190"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEX4994734.1"
                     /translation="MDGVHDLGGMQGFGPVEREENEPTFHAAWEAVVLAMQHAGRARG
                     LFNIDEFRHGIERMAPAHYLSATYYEKWLEGITRLLVEKGVVGAEELATRRTFFEDKP
                     DAPAAAPLSGPPPARGASNPRWAQDFIRETGATPRFAPGDPVRTREMHPHGHTRLPRY
                     ARGKRGVIHLCHGVHVFPDTNAHGRGEQPQPLYSVRFDARVLWGESAEPNQVVHIDLW
                     ESYLLPGR"
     gene            complement(2182..2748)
                     /locus_tag="VFX87_07195"
     CDS             complement(2182..2748)
                     /locus_tag="VFX87_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994735.1"
                     /translation="MTVEENLDGSRTEYFAIECTEACLLALLREVFEEHWEHVIFGPC
                     IEGAVFEGRFGRRPRLSLLDGYVTVQVDGSESWHFHLCIGPHRGSPELPTPPGLAEWR
                     RCTRAAFFQHLDRAGRPGSWGLRLWNGRGEQMLTVFMPNPWIDPARGRYVDTPDWSRL
                     ELWMALRARHAGVPAEPPPARAEPPRTH"
     gene            complement(2768..3292)
                     /locus_tag="VFX87_07200"
     CDS             complement(2768..3292)
                     /locus_tag="VFX87_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994736.1"
                     /translation="MPYHLAGTAWSPRRVLRERTAHCLEGAVFAAAALRVLGFPPLLL
                     DLEAVQDTDHVVAVFRSRGHWGAIAKSNFSGLRYREPVHRSLRELVMSYFDGYINLRG
                     DRTLRAWAGPVNLARFDRTRPGWMTTEEDLWWIAEYLLEIPHTRLLRPAMVRALSRVD
                     RRALAAARVGQREH"
     gene            complement(3394..4527)
                     /locus_tag="VFX87_07205"
     CDS             complement(3394..4527)
                     /locus_tag="VFX87_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994737.1"
                     /translation="MNYRIISADDHIDMQWLPRDLWQKRVPAAWRERAPRVVETDAGP
                     YWVCGDERWDSWGGRRGVAGSMGGRRLALEKGGVLEPGVLRPTTTALRLADMDRDGVD
                     ATVMYGPIVPLLIKDPALRQVCYHAYNEWLAEFCATAPERLIGAGLIPVDEPKVAADE
                     VRSLAQLGLRTGMFLAARAELPLWDAAWEPLWEAAAETGLPIGFHLGGGLRTVTFSGP
                     KAADPGNMGVRVACATLQMDEPLAAVIFSGALERHPGLRIVLAETGIGWLPYMLERMD
                     DTYRRFVDAAEYWRAHGGLRLTMPPSEYWKRQVWATFQLDPVGLHLVDVLGAGRVMWA
                     SDYPHADSTWPDSQPAIEESFRGVPAATRRRILCDNARELYRL"
     gene            complement(4568..5845)
                     /locus_tag="VFX87_07210"
     CDS             complement(4568..5845)
                     /locus_tag="VFX87_07210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4994738.1"
                     /translation="MTRPSHDADVLVVGGGPGGSTAAAFLAQGGLRVTLAEREAFPRF
                     KVGESLIPTCMDICRRLGVLDRVLAHGFQMKYGATFHDQELGLSSTFDFKPGRPWPAF
                     TLDVHRAEFDQILLDHAVAQGVTRLQPATVEKVAFDADGVTARLSLGEAERELRARFL
                     VDASGRDAFLAARQGQRKPRPGLGKVAIFAYFRGARRFPGREEGHVRIHIFPEGWFWY
                     IPLARDETSVGCVLHQKVVKARRGSLQELFDDMVERCHAVRDNLQGSSPVTELYTTSN
                     FAYSVEPVVGDRFLCVGDAVAFVDPIFSPGVFLAMQSGELAAGAILRAFRADRFEARR
                     FRAYERAVRRGTRPFERFIESFYDRAFLEVFLHPRNLLGMVPAVTGVLAGGSFGTLPK
                     HLRLSLWGFFQVVRFTRWLNLRRGVVLESRLDW"
     gene            5947..6381
                     /locus_tag="VFX87_07215"
     CDS             5947..6381
                     /locus_tag="VFX87_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4994739.1"
                     /translation="MTDIRIVSEPHASESLKQVVVDHLDTYNVAVTGFHEYSPVNLFL
                     RDAGDEVVGGLLAAIWGGALFVRILWVSEALRGQGFGRRLMEMAERRGMERGCRHVFV
                     DTFSFQAPGFYAKLGYRTYARAEDWPVGHAHHFLRKDLSPAP"
BASE COUNT          952 a         2315 c         2318 g          870 t
ORIGIN      
        1 cggaaggggg gcatagcccc cctccgagtc aaaacacctc gtcgcgttcg ccgaactcga
       61 actggtaggt ggtctcgtcc agctcggtgt cggtgatgag ctgcttgcgg gcgaggaggg
      121 cttcgaaggt ctggagccag atctcgtagt agacgaaggg gccgctgcgc gcttccgcgg
      181 ccttgatctg ggcgatgagg gtctggcgga actcctccca ctcgtagagg ccgcgctcgt
      241 gcagcgccac cgccatgccg aagacgcggc cctgccaggg ctcctcgaag accagctcgc
      301 cgttcttgcg cggcagggcg gcctggccct ccatgaccgc gaccacgcgg tccaccgtga
      361 ggtcctggcc ctccgcggtc atccccgagg gcgtggactc gcaggcgtgg gcccggcgcc
      421 cacgccagag gcgggcgtcg tccaccggct gaccgtcggg tcgcgactgc caggcggagg
      481 cggtgaactg gtacggcgcg agcgcgggcg gcggaggcag gcaggcgtcg gcgtccagca
      541 tcgcctcgac gtgatcgtag agcgcgacgg cgagcgcatc cgagtcgatc gagtggaacc
      601 actgggcctc gccgtcgaag agcacgagtg ccacgttggc gagcgcgcag gggcccaggc
      661 agccgccgat ggtgaggtgg atgatgttcc ggagccgccg ccgctcccat tcgcggtgga
      721 aggtctcggt ggggaccggc gcgaagccgt cctcggtacg gccgcagcag cagccggtgg
      781 cgcagacgaa gagctggccc cggaagcgcg ccacgttcac gcggcgccca tcgcggcgca
      841 ccacgatgtt gcgccggcgc tcgtacacgg agccgtcgcc gctcaggagg cggcggtcgg
      901 gcgcggctcg gccacgccga tgagcgcgtc gcgggtgatc agcggcacca gctccgcttc
      961 gcccatgttc tcggtgcccg ccggccgacg cggcagcacc atgtagcgca tctccgcgct
     1021 cgagtcccac acgcggatct cgcgctccgc cggcaccgcg agccccatct cctggagcat
     1081 cgtccgcggc tcgctgaccg cgcgggagcg gtaggccggc atcttgtacc aggtgggcgg
     1141 cagccccagc accggccacg ggtagcagga gcagagcgtg cacacgacca ggttgtgtac
     1201 cgcgtccgtg ttctcgacca ccatcagcat ctcgtagtcg aggtcaaatc gcgcgagcgc
     1261 ggctttggag tcgtccagga gcaggcggcg gtagtcggga tccgtccacg ccttcgccac
     1321 cgcgcgggcc cccaccatcg ggccggtgtc cgcctcgtag cgctggatca cccggtccac
     1381 cgcgtcgggg gccaggatgc ccttctcgag cagcagcgac tcgagcgccc gcacgcggga
     1441 gacgatgtag ggatcgacgt tggggtggtt cttgcccatc cgctttacct cccgggcagg
     1501 agatagctct cccagagatc gatgtgcacg acctggttcg gctccgccga ctccccccac
     1561 aggacccgcg catcgaagcg cacgctgtag agcggctgcg gctgctcgcc ccggccgtgg
     1621 gcgttggtgt cggggaagac gtggacgccg tggcagagat gaatcacccc gcgcttgccg
     1681 cgcgcgtagc gcggcagccg cgtgtgcccg tgcgggtgca tctcacgcgt gcgcacggga
     1741 tcgcccggcg cgaagcgtgg cgtggccccg gtctcgcgga tgaagtcctg ggcccatctc
     1801 ggattggagg cgccccgcgc gggtggcggc cccgagagcg gtgccgccgc gggcgcgtcc
     1861 ggcttgtcct cgaagaaggt ccggcgcgtc gccagctcct ccgcgcctac cacccccttc
     1921 tcgacgagaa ggcgcgtgat cccctcgagc catttctcgt agtaggtcgc gctcagatag
     1981 tgggcgggag ccatgcgctc gatgccgtgg cggaactcgt cgatgttgaa gagcccgcgc
     2041 gcgcggcccg cgtgctgcat ggccagcacc accgcctccc aggccgcgtg gaacgtgggc
     2101 tcgttctcct cgcgctcgac cggcccgaag ccctgcatcc cccccagatc gtgtaccccg
     2161 tccatacgcc gtgattctac ctcagtgggt gcgcggaggc tcggcgcggg cgggcggggg
     2221 ttcggcgggc acaccggcat ggcgggcgcg cagggccatc cacagctcga gccgggacca
     2281 gtcaggcgtg tccacgtagc gacctcgcgc ggggtcgatc cacgggttcg gcatgaagac
     2341 ggtgagcatc tgctcgccgc ggccgttcca gaggcgcagg ccccaggagc cgggccgccc
     2401 ggcccggtcg aggtgctgga agaacgcggc gcgggtgcag cggcgccact cggccaaccc
     2461 gggcggggtg ggcagctcag gcgagccccg gtgcgggccg atgcagagat ggaagtgcca
     2521 ggactcgctg ccgtcgacct gcacggtgac gtaaccgtcg agcagggaga gccggggacg
     2581 ccggccgaac cggccctcga acacggcgcc ctcgatgcac gggccgaaga tcacgtgctc
     2641 ccagtgctcc tcgaacacct cgcggagcag ggcgagcagg cacgcctccg tgcactcgat
     2701 cgcgaagtac tcggtacggc tgccgtcgag attctcctcc acggtcatgt cggcgctccc
     2761 gccggggtca atgctcacgc tggcccaccc gcgcggcggc gagggcacgc cggtccaccc
     2821 gcgagagagc gcgcaccatg gcaggccgca gcagccgcgt gtgcggaatc tcgagcaggt
     2881 actcggcgat ccaccagaga tcctcctcgg tcgtcatcca gccgggccgc gtccggtcga
     2941 agcgggccag gttcaccggc cccgcccagg cgcgcagggt gcgatcgccc cgcaggttga
     3001 tgtagccgtc gaagtagctc atcaccagct cacgcaggct ccggtgcacc ggctcgcgat
     3061 aacgcagccc cgagaagttc gacttggcga tggcgcccca gtgcccgcgg ctccggaaca
     3121 ccgccaccac atgatcggtg tcctgcaccg cctccagatc gagcaggagc ggcgggaaac
     3181 cgagcacgcg cagggccgcc gccgcgaaca ccgcgccctc caggcaatgc gcggtgcgct
     3241 cgcgcagcac ccggcgcggc gaccacgcgg tgcccgccag atgatacggc atggcgtcga
     3301 gcgcccgctg gatgccgatg gggctccgga gcgaccgcag cgcacgcagc tcgcgcgggg
     3361 tgaagctcgg ggccggcgtg gagcgggctg ggttcagagc cggtagagct cccgggcatt
     3421 gtcgcacagg atgcgccgcc gcgtcgccgc gggcacgccc cggaagctct cctcgatggc
     3481 cggctgcgag tcgggccacg tgctgtcggc gtgcgggtag tcggaagccc acatcacgcg
     3541 gcccgcgccg agcacgtcga ccagatgcag accgaccggg tcgagctgga aggtggccca
     3601 gacctggcgc ttccagtact cgctgggcgg catggtgagt cgcagcccgc cgtgcgcgcg
     3661 ccagtactcg gccgcgtcca cgaaacgccg gtaggtgtcg tccatgcgct cgagcatgta
     3721 gggcagccag ccgatcccgg tctcggccag cacgatgcgc agcccggggt gacgctcgag
     3781 cgcgcccgag aagatcaccg cggcgagcgg ctcgtccatc tgcagcgtcg cgcacgccac
     3841 ccgcacgccc atgttgccgg gatcggcggc cttgggcccg ctgaaggtca cggtgcgcag
     3901 cccgccgccc aggtggaagc cgatcggcag cccggtctcc gcggcggctt cccacagcgg
     3961 ctcccacgcc gcgtcccaga gcggcagctc ggcccgcgcc gcgaggaaca tgccggtgcg
     4021 cagcccgagc tgggcgagcg agcgcacctc gtcggcggcg accttgggct cgtcgaccgg
     4081 gatcagcccg gcgccgatca gccgctcggg cgcggtggcg cagaactcgg cgagccactc
     4141 gttgtaggcg tggtagcaga cctggcgcag cgcgggatcc ttgatcagca gcggcacgat
     4201 gggcccgtac atcacggtgg cgtccacccc gtcgcggtcc atgtcggcca ggcgcagcgc
     4261 ggtggtggtc gggcgcagca cgccgggctc gagcacgccg cccttctcga gcgcgaggcg
     4321 ccgcccgccc atggagccgg ccacgcctcg gcgcccgccc caggaatccc agcgctcgtc
     4381 cccgcagacc cagtagggcc cggcgtccgt ctccaccacc cggggcgcgc gctcgcgcca
     4441 cgccgccggc acccgcttct gccagagatc gcgcggcagc cactgcatgt cgatgtggtc
     4501 gtcggccgag atgatccggt agttcatgcg cgcggtctcc atgtgcaggg tcggggacgg
     4561 gctcgggcta ccaatcgagg cgggactcga ggaccacgcc gcggcgcagg ttcagccagc
     4621 gggtgaagcg caccacctgg aagaaccccc agagcgacag gcgcaggtgc ttcggcaggg
     4681 tgccgaacga gccgcccgcg agcacgccgg tgaccgccgg gaccatgccc agcaggttgc
     4741 gcgggtgcag gaagacctcg aggaaggcac gatcgtagaa ggactcgatg aagcgctcga
     4801 aggggcgcgt gccccgccgg accgcgcgct cgtaggcgcg aaagcgcctg gcttcgaacc
     4861 ggtcggcgcg gaaggcgcgc aggatcgccc ccgcggccag ctcgcctgac tgcatcgcca
     4921 ggaacacccc gggcgagaag atcgggtcca cgaaggccac cgcgtcgccc acgcacagga
     4981 accggtcgcc caccaccggc tccaccgagt aggcgaagtt cgaggtggtg tagagctccg
     5041 tgaccggcga agatccctgg aggttgtcgc gcaccgcgtg gcagcgctcg accatgtcgt
     5101 cgaacagctc ctggagcgag ccgcggcgcg ccttgaccac cttctggtgg agcacgcatc
     5161 ccacgctcgt ctcgtcgcgc gcgagcggga tgtaccagaa ccagccctcc gggaagatgt
     5221 ggatgcggac gtggccttcc tcgcggcccg ggaagcggcg ggcgccgcgg aagtaggcga
     5281 agatggccac cttgcccagg cccggccgcg gcttccgctg cccctggcgc gcggccagga
     5341 aggcgtcgcg cccgctcgcg tccaccagga agcgcgcgcg cagctcgcgc tcggcctcgc
     5401 ccaggctcag gcgcgcggtg accccgtcgg cgtcgaaggc caccttctcg acggtggcgg
     5461 gctgcaggcg cgtcaccccc tgcgccaccg cgtgatcgag gaggatctgg tcgaactcgg
     5521 ctcggtgcac gtcgagcgtg aaggcgggcc acggccggcc gggcttgaag tcgaaggtgg
     5581 aggagaggcc cagctcctga tcgtggaagg tggcgccgta cttcatctgg aagccgtgcg
     5641 cgagcacccg gtcgagcacg cccagccggc ggcagatgtc catgcacgtc gggatgaggg
     5701 actcgccgac cttgaagcgc gggaaggcct cacgctccgc cagggtgacg cgcagtccgc
     5761 cctgggcgag aaaggccgcg gcggtgctgc cgcccggccc gccgcccacg accagcacgt
     5821 cggcgtcatg cgaaggcctg gtcacgcccg gcatcttacc cgatcccccg ggcaacccgc
     5881 gagccctcgg gcggcgggtt tgccgccccg cgtcgggcat gctaggttcg gcgcctggga
     5941 gcgccgatga ccgacatccg gatcgtgtcc gagccccatg cctccgagag cctcaagcag
     6001 gtcgtggtgg accacctgga cacctacaac gtggcggtca ccggcttcca cgagtactcc
     6061 ccggtcaatc tcttcctccg ggacgcgggc gacgaggtcg tcgggggcct cctcgcggcg
     6121 atctggggcg gcgccctctt cgtccgcatc ctctgggtca gcgaagcgct gcgcggccag
     6181 ggcttcggcc ggcggctgat ggagatggcc gagcggcgcg ggatggagcg cgggtgccgc
     6241 cacgtcttcg tggacacctt cagcttccag gctcccggct tctacgcgaa gctcggctac
     6301 cggacctacg cccgggcgga ggactggccg gtcgggcatg cccaccattt cctcaggaag
     6361 gatctctcgc cggcaccgtg atcaagctcg tgttctgcct ccgccgcctg ccccacctgt
     6421 cccgcgagga gttccagcgc tactggctcg acacc
//
LOCUS       DASUFA010000201         7266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1417086, whole genome shotgun sequence.
ACCESSION   DASUFA010000201 DASUFA010000000
VERSION     DASUFA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417086"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFX87_07220"
     CDS             complement(<1..890)
                     /locus_tag="VFX87_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4994740.1"
                     /translation="MTLDEKVHGLRLHVIRRAEALGNVSRACREAGISRALFYRWRGR
                     LERYGPDGVHPRRHQARPGRPVQLAPEIERVLLSVAVSAATWGASRIAAYLQHRWPLR
                     VAPSTVQRALRRAGLATRRQRLLVLEQRAVQTAGLLTERTRRTLWQAQHGQPRHVAAR
                     EPGELVCLDTFYIGQLKGVGKVWQITACDAACSYGVAWLLPAHTAAAAAPFLRRILLP
                     LYRRAGWRLRRVLTDGGPECKGLFDEACRTLGLRHTRTKPRHAWTNGFVERLQGTILQ
                     EHWRVAFRRRYFTSRPALQR"
     gene            907..2982
                     /locus_tag="VFX87_07225"
     CDS             907..2982
                     /locus_tag="VFX87_07225"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX4994741.1"
                     /translation="MPLPAEAAVSGYAFRIGARRIVGEVDRIAAARERFEEALLEGRT
                     AGLLEQDRSSLFTQELGNIPPGMEVVAELIIDQRLRWLEEGAWGWRFPTVVAPRYLGG
                     PGQVADAPRVTVDVADGPLPVVSHLAFTIGDPLSGAHEMHSPSHGLKIAAVPAGHEIG
                     LAAAGAALDRDLVVHWQSARSHVVATLETGRPAAGRPPADAAYGLLTLTPPLPAARPP
                     ALPRDLIVLLDMSGSMAGEPLDQARRVVSRVIDTLGDVDQLEIIGFASEAKRWKPSPR
                     MATASAKRSAVQWLEKLAADGGTEMRVGIAEALRPLRVNAQRQIVLVTDGQIGFESEV
                     VGMIARDLPRSCRLHTVGVGPAVNRTLTSGAARAGRGGEIVVGLGENPMPAVARLLAR
                     LELPLLTDLALTGSALLHHVPAGLPDVHAGAPALIGLRLRPEGGELRITGRLGGRQWE
                     EMLSVPAVAAGAGNAAVVACYGREAVEDLEMRVSAGEMAALDQEIETVGLQYQIATRL
                     TSWFAATEEATVDPQQALRRERIPQALPAGLSAAGLGLRAHEAMYHLAMPMVMRMSAS
                     VAPRPTASRRLAERVPDAMRGARPRPKRGHELRGRVVLRRYRELVVEITLDRPLAWDP
                     SDARAVWDDGTECGAAIDASRSTRAGAVEAGNVIRLVLRLDETALPSAPARILLVSKS
                     GSLDIVLDP"
     gene            2979..3581
                     /locus_tag="VFX87_07230"
     CDS             2979..3581
                     /locus_tag="VFX87_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4994742.1"
                     /translation="MTVGQARLPDLDEHLPRIVAGEVGAFGSWMAGAEARLRDSLRSF
                     AVRVDVEAVVQETLLRVWQTAPRFVADGRENGLLRFAVRVARNLAVSELRRYRAEPME
                     IGALEREAEAYDPIGSEGPRARDPLLARRIDDCLKRLPRQPGRALGARVEGLGVEPDE
                     TLAAGLGMRTNTFLQNVTRARKLIAECLERHGVDLAAELA"
     gene            3578..3814
                     /locus_tag="VFX87_07235"
     CDS             3578..3814
                     /locus_tag="VFX87_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994743.1"
                     /translation="MRQGARRAILIEAATTAWRPRSPSGELRPHPAWSDLDPAGRLEA
                     YEATRAVRRLEAARHPEGMTGTTRAVLARLAGMR"
     gene            complement(3890..4033)
                     /locus_tag="VFX87_07240"
     CDS             complement(3890..4033)
                     /locus_tag="VFX87_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994744.1"
                     /translation="MRARKVLLGVLLTAAAAGLFSGCGTMAQGPTYTDAELRAICERR
                     GGW"
     gene            complement(4102..5082)
                     /locus_tag="VFX87_07245"
     CDS             complement(4102..5082)
                     /locus_tag="VFX87_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4994745.1"
                     /translation="MTIRIAAIEVGHWHAVHDAAYLHHLAAMPDVALVGVQDSDAALA
                     KKRAGAVGNPETFTDYPQMLAETKPDFVLGLGRHRQMARIAHDLLDRGLPFMMEKPMG
                     ISAAEVQTIAEKATRVKGYAAVPLFQRYQPFAVRARQLLAEGRFGPVSHIYVRINRPA
                     PARYPAWDSAWMLDPAEAGGGCLRNLGSHGLDMFLYLTGEDAQVTGAQISTRAHGQRV
                     EDYASVLLRSSTGILGTVEVGNGFPRVGTDGEWKIAGRDAILTLKDGVLKLATAQGDE
                     ITPCPDDEVRYFPAIRDILDAWQRGAAPPISVHDCLRAVRLIDQAYALAT"
     gene            5226..5540
                     /locus_tag="VFX87_07250"
     CDS             5226..5540
                     /locus_tag="VFX87_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994746.1"
                     /translation="MHVAKVAVIGIVALAVAAPAVAQETRVDRRQERQEQRIDKGVQS
                     GALTPRETRKLERGQKRVENLETKAQADGKVTAREKARLEHAQNVQSKRIYKQKHDAQ
                     TR"
     gene            complement(5541..6584)
                     /locus_tag="VFX87_07255"
     CDS             complement(5541..6584)
                     /locus_tag="VFX87_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX4994747.1"
                     /translation="MKTIVALPGEGIGPEVVDATCELLIGTGLPLKILTPPQGSPLPE
                     ETKRAAREADGVLFGAAGPSTSPVVSWLRWEMAAWAGVRPIRFFPGMRSPLADPDGID
                     YVLLRENSEGLYPGREGDLAELVKLMPGLADRTGRTIKDFGTEGRFAVKVVTPRGAQR
                     IARFACDLARRRQKRGKPGKITLVTKSNVLPRTDGLYDEIVANVVREAGLPFEHFHVD
                     DAARRLVRFPKAMDVVLCMNLYGDILSDLGAETAGGLGLAPSGNFGDGWAYFESVHGS
                     APDIAGKGIANPTATILSAALMLEHMGLSAEAQRLEAAVGRVYRDGKVLTPDQGGAAR
                     TRELAQAVLAAYR"
     gene            complement(6636..>7266)
                     /locus_tag="VFX87_07260"
     CDS             complement(6636..>7266)
                     /locus_tag="VFX87_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994748.1"
                     /translation="LCVKDGEPGRIFWTRDTVFTGISAPGNCKSRLVAREVELTLVGH
                     SMGALVVNRLLLGFPEVPFRRVVFLGAAASIDELDTAVLPFLDRHPMAEVHNVSLSMN
                     DERYEKGWGDLWERGSLLVWIDNLFERVNSAPQLRFGRCLNLFRPALGEPAATRLRYD
                     QRLFFHKLKGRDEDRDPRRHGDFDDKDKLPVILTMVEKSPGEAPRCAAP"
BASE COUNT          996 a         2576 c         2707 g          987 t
ORIGIN      
        1 ctgcgctgca gcgccgggcg actggtgaag tagcggcgcc ggaaggccac gcgccagtgc
       61 tcttgcagga tcgtgccttg cagccgctcg acgaacccat tggtccaggc gtgccgcggc
      121 ttcgtccgag tgtgccggag gccgagggtg cgacaggcct catcgaagag gcccttgcac
      181 tcggggccgc cgtcggtgag cacccgccgc agccgccagc ccgcgcggcg atagagcggg
      241 agcaggatcc ggcgcaggaa gggggcggcc gccgcggcgg tgtgggcggg cagcagccac
      301 gccaccccgt aggagcacgc ggcatcgcag gctgtgatct gccagacctt gcccacgccc
      361 ttgagctggc cgatgtagaa ggtgtccagg cacaccagct cgccgggctc gcgggcggcc
      421 acatgtcgcg gctgcccgtg ctgggcttgc cagagggtgc ggcgagtccg ttcggtgagc
      481 aggcctgccg tctgcacggc gcgctgctcg agtacgagga gccgctgccg gcgtgtcgcc
      541 aaccccgcgc gccgcagggc ccgctgcaca gtgctggggg ccacgcgcag cggccagcgg
      601 tgctgcaggt acgccgcgat ccggctcgcg ccccacgtcg cggcgctcac cgccacgctc
      661 agcagcaccc gctcgatctc cggcgccagc tgcaccggcc ggccggggcg ggcctggtgt
      721 cggcgggggt gcacgccgtc cggtccgtag cgttccagcc gcccgcgcca ccggtagaag
      781 agggctcggg agatcccggc ctctcgacac gcccggctga cattgcccag tgcctccgcc
      841 cgccggatga catgcagccg caggccgtgt accttctcgt cgagggtcat cggtcgaacc
      901 taccagatgc cgctccccgc ggaggccgcc gtctcgggct acgccttccg catcggcgcc
      961 cgccgcatcg tgggcgaggt ggatcggatc gcggcggcgc gcgagcgctt cgaggaggcg
     1021 ctgctcgagg gccgcaccgc ggggctgctc gagcaggacc gctcgagcct cttcacccag
     1081 gagctgggca acatcccgcc cggtatggaa gtggtcgccg agctgatcat cgaccagcgg
     1141 ctgcgctggc tcgaggaggg cgcctggggg tggcgcttcc ccaccgtggt ggcgccgcgc
     1201 tacctgggcg gaccggggca ggtggccgac gccccgcgcg tcaccgtgga cgtggccgac
     1261 ggcccgctgc cggtggtctc gcacctggcc ttcacgattg gcgacccgct gagcggcgcg
     1321 cacgagatgc actcgccctc gcacggcctg aagatcgccg cggtgcccgc gggccacgag
     1381 atcggcctcg ccgccgccgg cgcggcgctc gatcgcgacc tggtcgtcca ctggcagagc
     1441 gcgcggtccc acgtggtcgc cacgctcgag accggacgcc cggcggccgg ccggccgccc
     1501 gccgacgccg cctacgggct cctgacgctc acgcccccgc ttcccgccgc gcgcccgccc
     1561 gcgctgccgc gcgatctcat cgtcctgctc gacatgagcg gctccatggc gggcgagccc
     1621 ctggaccagg cccgccgtgt cgtctcgcgc gtgatcgaca cgctgggcga cgtcgaccag
     1681 ctcgagatca tcgggttcgc gagcgaggcc aagcgctgga agccgagccc gcgcatggcc
     1741 acggcctccg cgaagcgcag cgccgtccag tggctggaga agcttgccgc cgacggcggc
     1801 accgagatgc gcgtgggcat cgccgaggcg ctgcgcccgc tccgcgtgaa tgcgcagcga
     1861 cagatcgtgc tggtcaccga tggccagatc ggcttcgagt cggaggtcgt cgggatgatc
     1921 gcccgcgatc tgccccggtc ctgccggctc cacacggtgg gcgtcggccc cgcggtgaat
     1981 cgcacgctga cgagcggggc cgcgcgggcg gggcgcggcg gcgagatcgt cgtcggcctg
     2041 ggcgagaacc cgatgcccgc ggtggcgcgg ctgctggccc ggctcgagct gccgctcttg
     2101 accgacctcg cgctgacggg atcggcgctg ctccatcacg tgcccgcggg gctccccgac
     2161 gtccatgccg gcgcgccggc cctgatcgga ctgcggctcc ggcccgaggg cggcgaactg
     2221 cgaatcacgg ggcggctcgg cggccgacag tgggaggaga tgctgagcgt tccggcggtg
     2281 gccgccggag cggggaacgc ggcggtggtc gcctgctacg ggcgcgaggc cgtcgaggat
     2341 ctggagatgc gcgtctcggc gggtgagatg gcggccctcg accaggagat cgagacggtc
     2401 gggctgcagt accagatcgc gacgcggctg acctcgtggt tcgcggcgac ggaggaggcg
     2461 acggtcgatc ctcagcaggc gctccggcgg gaacggattc cgcaggcgct gcccgcgggg
     2521 ctctccgcgg ccgggctcgg gctgcgcgcg cacgaggcca tgtatcacct ggcgatgccc
     2581 atggtcatgc ggatgtctgc gtcggtggct ccgcggccga cggcatctcg ccgcctggcc
     2641 gagagggtcc cggacgcgat gcgcggcgcg cggccccgcc cgaagcgggg tcacgagctg
     2701 cgcgggcggg tcgtgctccg gcggtaccgc gagctggtgg tggagatcac gctggacagg
     2761 cccctggcat gggatccgtc cgacgcgcgc gcggtctggg acgacggcac cgagtgcggc
     2821 gctgcgatcg acgcgagccg atccacgcgg gcgggcgcgg tggaggcggg caacgtgatc
     2881 cggctcgtgc tgcggctgga cgagaccgcg ctccccagcg cgcccgctcg gatcctgctc
     2941 gtctcgaagt ccggctcgct ggacatcgtg ctcgacccgt gacggtcggc caggcgcggc
     3001 tgccggatct cgacgagcat ctcccgcgca tcgtggccgg agaggtcggg gcgttcggtt
     3061 cctggatggc cggcgccgaa gcgcggctgc gcgatagcct gcggtcgttt gcggtccggg
     3121 tggacgtgga ggccgtcgtg caggaaacgc tgctgcgggt ctggcagacc gccccgcgct
     3181 tcgtggccga cgggcgcgag aacgggttgc tgcggttcgc ggtccgcgtg gcgcggaacc
     3241 tggccgtctc ggagctccgg cgctaccgtg ccgagcccat ggagatcggt gcgctcgagc
     3301 gggaggcgga ggcatacgat cccatcggat cggagggccc gcgcgcccgg gacccgctgc
     3361 tggcgcgccg catcgacgac tgtctcaagc gcctgccgcg ccagcctggc cgtgcgctcg
     3421 gggcacgtgt ggagggcctg ggcgtcgagc ccgacgagac gctggccgcc gggctcggca
     3481 tgcggacgaa cacctttctc cagaacgtca cgcgcgcgcg gaagctcatc gccgagtgcc
     3541 tggagcggca tggggtcgat ctcgcggcgg agctggcatg agacagggag cgcggcgggc
     3601 gatcctgatc gaggccgcaa cgacagcctg gcggccgcgg tctccttccg gcgagctccg
     3661 tccgcacccg gcgtggagcg acctcgatcc cgcgggccgc ctggaagcct atgaagccac
     3721 gcgggccgtc cgccgtctgg aagcggcgcg ccatcccgag ggcatgaccg gcaccacgcg
     3781 cgcggtgctg gcgcgactgg ccgggatgcg gtgagccgga tcccggccgc ggtcaggggg
     3841 cgagtgaagc cgactggaac tcgcagtagc ccgcgatcag gtcgccgcgc taccagccgc
     3901 cccggcgctc gcagatggcc cggagctcgg cgtcggtgta cgtcgggccc tgcgccatgg
     3961 tgccgcaacc gctgaacagg cccgccgccg cggcggtgag cagcactccg agtagaacct
     4021 tccgggctct cattccagga cctccccacc cgcaactatg atgcgagcgg gcgggttccg
     4081 gattcgagag ccgggaagtc cctacgtggc gagcgcgtag gcctggtcga tgagccgcac
     4141 cgcgcgcagg cagtcgtgca cgctgatcgg gggcgccgcg ccccgctgcc acgcgtcgag
     4201 gatgtcgcgg atcgccggga agtaacgcac ctcgtcgtcg gggcacggcg tgatctcgtc
     4261 cccctgggcg gtggccagct tcagcacgcc gtccttcagg gtgaggatgg cgtcgcgtcc
     4321 ggcgatcttc cattcgccgt cggtgcccac gcgcgggaag ccgttgccga cctcgacggt
     4381 gcccaggatc ccggtgctcg agcgcagcag caccgaggcg tagtcctcca cgcgctggcc
     4441 gtgggcgcgc gtgctgatct gggcgccggt cacctgcgcg tcctcgccgg tcaggtacag
     4501 gaacatgtcg aggccgtgcg agccgaggtt gcggagacag ccgccgcccg cctcggccgg
     4561 gtcgagcatc cacgccgagt cccacgccgg gtagcgcgcc ggcgcggggc ggttgatgcg
     4621 cacgtagatg tgggagacgg gaccgaagcg cccctcggcc aggagctggc gggcgcggac
     4681 ggcgaagggc tggtagcgct ggaagagcgg caccgcggcg tagcccttca cgcgcgtggc
     4741 cttctccgcg atggtctgca cctccgcggc gctgatgccc atcggcttct ccatcatgaa
     4801 gggcagcccg cggtcgagga gatcgtgggc gatcctcgcc atctgccggt gacgtcccag
     4861 gcccagcacg aagtcgggct tcgtctccgc cagcatctgg gggtagtcgg tgaaggtctc
     4921 cggattgccc accgcccccg cccgcttctt cgcgagcgcg gcgtcactgt cctgcacgcc
     4981 gaccagggcc acgtcgggca tcgcggcgag gtggtggaga taggcggcgt cgtgcacggc
     5041 gtgccagtgc ccgacctcga tggcggcgat gcgaatggtc atgaaatcct ccgcggggga
     5101 cgcaggatgt ggggccgctt cggacggcct gagcgggggc cgtaaacgct cctgggccta
     5161 aatccgtctg acggacaata gctgataggg ccgggtccgg cccaaccgcc aggaggaaca
     5221 gtccaatgca cgttgccaag gtcgctgtca tcgggatcgt cgcgctcgcc gtcgccgccc
     5281 cggccgtcgc ccaggagacg cgggtcgacc gccggcagga gcgccaggag cagcggatcg
     5341 acaagggcgt ccagtccggc gcgctgaccc cccgcgagac caggaagctc gagcggggcc
     5401 agaagcgcgt cgagaacctc gagacgaagg cccaggccga cggcaaggtc accgccaggg
     5461 agaaggcccg gctcgagcac gcgcagaacg tccagagcaa gcgcatctac aagcagaagc
     5521 acgacgccca gacccgctag ctagcggtac gcggccagga ccgcctgcgc gagctcgcgc
     5581 gtcctggccg cgccgccctg gtcgggcgtg agcactttcc cgtcgcggta gacgcgcccc
     5641 accgcggctt cgagccgctg ggcttccgcg ctcagcccca tgtgctccag catcagcgcg
     5701 gccgacagga tcgtcgcggt gggattcgcg atgcccttgc ccgcgatgtc gggcgccgag
     5761 ccgtggacgg attcgaagta ggcccaccca tcgccgaagt tgccggacgg ggccagcccg
     5821 aggccgccgg ccgtctccgc gccgaggtcg gagaggatgt cgccgtagag gttcatgcac
     5881 agcaccacgt ccatcgcctt cgggaagcgc acgaggcgcc gcgcggcgtc gtcaacgtgg
     5941 aagtgctcga agggcagccc ggcctcgcgc acgacgttcg cgacgatctc gtcgtagagc
     6001 ccgtcggtgc gcggcagcac gttcgacttg gtcaccagcg tgatcttgcc cggcttgccg
     6061 cgcttctgcc gccggcgcgc caggtcgcag gcgaagcggg cgatgcgctg ggccccgcgc
     6121 ggggtgacca ccttcaccgc gaagcgcccc tcggtgccga agtccttgat ggtgcgcccg
     6181 gtccggtcgg ccagacccgg catgagcttg accagctcgg ccaggtcgcc ctcgcggccc
     6241 gggtagaggc cctcgctgtt ctcgcgcagc agcacgtagt cgatgccgtc gggatcggcc
     6301 agcggcgagc gcatccccgg gaagaagcgg atcggccgca cgcccgccca cgcggccatc
     6361 tcccagcgga gccaggacac cacgggcgag gtggacggcc ccgccgcgcc gaagagcaca
     6421 ccgtcggcct cgcgggccgc gcgcttggtc tcctcgggca ggggcgagcc ctggggcggc
     6481 gtgaggatct tgagcggcag ccccgtgcca atcagcagct cgcacgtggc gtccaccact
     6541 tcgggaccga tgccttcacc cggcagcgcg acgatcgtct tcatgggcca cccccctcct
     6601 ggggtcgggg cttacactac gatattcgtg agcggtcagg gcgccgcgca gcgcggggct
     6661 tcgcccgggg acttctccac catcgtcagg atgacgggca gcttgtcctt gtcgtcgaag
     6721 tcaccgtggc gccgcggatc ccggtcctcg tcgcgcccct tcagcttgtg gaagaagagg
     6781 cgctgatcgt agcgcaggcg ggtggccgcg ggctcgccga gggccggccg gaacaggttc
     6841 aggcagcgcc cgaaccggag ctgcggcgcc gagttgacgc gctcgaagag gttgtcgatc
     6901 cacacgagca gactgccgcg ctcccagaga tcgccccagc ccttctcgta gcgctcgtcg
     6961 ttcatggaga ggctcacgtt gtgcacctcg gccatcgggt gccggtcgag aaagggtagc
     7021 accgcggtat cgagctcgtc gatgctcgcg gcggcgccca ggaagaccac ccggcggaac
     7081 gggacctcgg ggaagccgag caggagccgg ttcaccacca gcgcgcccat gctgtggccg
     7141 accagcgtca gctcgacctc gcgggcgacg aggcgactct tgcagttgcc cggcgcggag
     7201 atcccggtga agacggtgtc gcgagtccag aagatccgcc cgggctcgcc gtccttcacg
     7261 cagagc
//
LOCUS       DASUFA010000202         9284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1172193, whole genome shotgun sequence.
ACCESSION   DASUFA010000202 DASUFA010000000
VERSION     DASUFA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172193"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /gene="pnp"
                     /locus_tag="VFX87_07265"
     CDS             complement(<1..1388)
                     /gene="pnp"
                     /locus_tag="VFX87_07265"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX4994749.1"
                     /translation="MQIQINNTPLTIETGKVAKQADGAVVVRCGGTMVLSTAVASRSA
                     LEGRDFLPLTVEYRERSYAGGRIPGGFFKREGRPDEKETLTCRLIDRPLRPLFPKGFR
                     NEIQIINLVISADSDNDPDVLAMIGSSAAMSLSGIPFSGPVGAVRVALVDGKLVANPT
                     YKQVAASPLELVMAGTEDAILMVESGGKEVSEEQMLEALAFGHERCKELTRIQKDLVA
                     KAAKPRWAFDAQAGADPALEARVKDLASAKVVQALSVHEKQARAQGLDAIFEEVFAAI
                     GGDDAVRAKARGYFEKVEKAEVRRLIVEKGIRVDGRSVKEVRPIWSEVAYLPRAHGSA
                     LFTRGETQALVSATLGTKDDEQKIEAFEGNAFRRFMLHYNFPSFSTGEVKRFGTPGRR
                     EIGHGALAQRAVEAVMPTREEFPYTVRVVSDILESNGSSSMATVCGASMALMDAGAPL
                     KAPVAGIAMGLV"
     gene            complement(1512..1781)
                     /gene="rpsO"
                     /locus_tag="VFX87_07270"
     CDS             complement(1512..1781)
                     /gene="rpsO"
                     /locus_tag="VFX87_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033016867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX4994750.1"
                     /translation="MRLSQDKKKGLIEQFKVHEGDTGSPEVQIALLSERINGLTEHFK
                     LHKKDHHSRRGLLMLIGKRRGLIEYLRKKDSERYRAITEKLGIRK"
     gene            complement(1928..2863)
                     /locus_tag="VFX87_07275"
     CDS             complement(1928..2863)
                     /locus_tag="VFX87_07275"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX4994751.1"
                     /translation="MRILRDLQSFPPELRPSVAAFGAFDGIHLAHAKILDTAVARARA
                     LGVSAVACTFDPHPTAVLRPEKAPAPIATLEENLARIAEHGLDATLVIPFTLDFSRLE
                     AETFVDEALVRTLGAREVVVGFNHTFGRGARGTAVLLRELGVHRGFVTHVLPPLQVNG
                     LTVSSSAIREALRDGDVEQARALLGRPYSLTGTVQRGAGRGRTLGFPTANLKPDRPLA
                     LAAGVYAAHARWDGRQAEAVVNIGYRPTFEESQYWVEAYLFDFAGDLYDRALTLDFLV
                     RIRAEMKFPGVEALTAQVRADMAEARRLLRAQPAG"
     gene            complement(2826..3743)
                     /gene="truB"
                     /locus_tag="VFX87_07280"
     CDS             complement(2826..3743)
                     /gene="truB"
                     /locus_tag="VFX87_07280"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX4994752.1"
                     /translation="MSDRHRAGILPVEKGPGVTSFQVVAHLRRVLRAPRIGHGGTLDP
                     AATGVLPILVGEATKLTPYLVDLDKEYLATVRLGVTTETQDLSGAVLETRPVPALDGP
                     ALEAALVPFVGRIRQVPPMYSALRRGGKRLYELARQGVEVEREPREVTVHSIRLESVA
                     LPDFVIRIRCGKGTYVRTLAADVGATLGCGAALAALVRTRVGPYALEAAVSWDEVREA
                     RSGERLWPRLLPPDTALAALAPVRLDADGARAFAHGQAVATGERVGGLLRVYGPDGAL
                     LGVGQGLGDRVKPERLLHADPPRPSVLPA"
     gene            complement(3740..4732)
                     /locus_tag="VFX87_07285"
     CDS             complement(3740..4732)
                     /locus_tag="VFX87_07285"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX4994753.1"
                     /translation="MSVTIVAPEPPAELVDLLARPGGSALMLGHVHPDGDVLGTLLGL
                     GLAMEKAGWTVTYGGPDPVPDVLDFIPGADRWQVWRTAPGTFDTIVLTDCPNDGRTEG
                     LLAGARGPGSRVVNIDHHPDNRRYGTLNWIDPSAAAVGEMIYDLLVALGWPVGRDTAL
                     GLYTAIHTDTGSFRYSNTTPRTFRIAGSLTAEGAEPALVTDRLYQRRPPDALLTLGSL
                     LSRVEVSEDGRIAALTVPEGAASEAFMAAEDLVTYPRSIAGVKVAVLLRAEGDGRVKA
                     SLRGKGDVPVNRIAHQFGGGGHENAAGCTLPGPLASAKATLLAAVRQALDGPAS"
     gene            complement(4729..5097)
                     /gene="rbfA"
                     /locus_tag="VFX87_07290"
     CDS             complement(4729..5097)
                     /gene="rbfA"
                     /locus_tag="VFX87_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX4994754.1"
                     /translation="MQGKRLVRVNQLIKEEISMVVQRQLKDPRLGFVTVTEVDTTPDL
                     KHAKVFVSVLGPDAQWAASFKALESARGFVWSWLRRHLDLRVTPEISFRPDRSMEHAA
                     HIQALLADLKRSEESSEDEA"
     gene            complement(5098..5388)
                     /locus_tag="VFX87_07295"
     CDS             complement(5098..5388)
                     /locus_tag="VFX87_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX4994755.1"
                     /translation="MSGARVAVGTVELHLPDVGSLKGKRHALKGLKDTLRRRFEISVA
                     EVDHHDVWQRATLALACVSGDSRHANEVISKAMDYIEDHVDGWVTDIQVEIL"
     gene            complement(5410..7908)
                     /gene="infB"
                     /locus_tag="VFX87_07300"
     CDS             complement(5410..7908)
                     /gene="infB"
                     /locus_tag="VFX87_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX4994756.1"
                     /translation="MAARVKVIDLAKELGVTSKDLIVALEGMGHKGMRAMSPLLAATA
                     NELRVKLGRGRDLPAEPKPKRAAKPKAAEGAAPAAPKKRAAGAKKTAAKSVEETPEVI
                     ELVPAPDAVKPAATIFRPAPSAVPPAPPAEIVPLPPVAPPRPASPPAPAPVVRPAAAA
                     PPVEVRPAPPVRRAPVPPPLNPPRTVISPPRPAAPPAPLRPAAPARPAAAAVAAPPPA
                     AAAIPAPPEVVPTAPRPAPGGALPPPPEPPQEIKRELIKLPESVTVGELAAAMRRKTT
                     EVIKALVDLGVMATVNEVLDPTAAKLVADKFHCDVQVRSLEGDVLEEEESDPAQMRPR
                     PPVVTVMGHVDHGKTSLLDAIRTTKVAEREFGGITQHIGAYQVDTSHGKVTFLDTPGH
                     EAFTAMRARGAQATDMVVLVVAADDGVMPQTVEAINHAKAANVPILVAVNKIDKPGSD
                     PDRVKRELSNHGLVPEDWGGQTIFIHTSAKRGDGIPQLLEMTALQAEILELRANPARA
                     GRGVIVEGRLDRGRGPVATALIQSGTLKEGDAVVVGSHSGRVRALFNDRGKKVQSAGP
                     SDPVEILGLSGVPQAGDTMLVVSDERKARQIATVRSERDRLKGKSATRITLEDLHKQI
                     EAGEVKELRLVLKADVQGSVEALTESLERLSTEEVKLKVIHSSVGTVTESDVMLASAS
                     NAIVLGFNVKADPKAATQAQANGVDMRSYNVIYDAINDVRAALSGMLAPEIREMVLGR
                     AQVRQLFPITKVGTIYGSSVTEGKMVRGARARIKRGETLLGEGVISSLKRFKDDVREV
                     LQGLECGVGIEGIKGVQPNDVIEAFSTEEVARTL"
     gene            complement(7914..8291)
                     /locus_tag="VFX87_07305"
     CDS             complement(7914..8291)
                     /locus_tag="VFX87_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX4994757.1"
                     /translation="MGGRASGAAGRERRGGRARGRGRWIGDRADPLEKLKRAQRPPQR
                     TCIGCRVVRAKAELIRLVRDEGGVVRVDRDGRAAGRGAYVCATTECVEKALQAGRLGH
                     AFKGASRPPQEPAVVILASRPRR"
     gene            complement(8186..>9284)
                     /gene="nusA"
                     /locus_tag="VFX87_07310"
     CDS             complement(8186..>9284)
                     /gene="nusA"
                     /locus_tag="VFX87_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX4994758.1"
                     /translation="ELEIELAPQEFGRIAAQTAKQVILQRVRDAERDAVYSEFIDREG
                     KIVRGTVHRIEKRNVVVELGKAEAVITEREQLPGDRFNPGDRVRAYMLEVKKTAKGPQ
                     ILLSRTHPGFLVRLFETEIPEIAEGIVLVKAAAREPGERAKVAVSSTKRDVDPIGACV
                     GLRGTRIQVISRELRGEKIDIVEWSPDSAVFVARALSPAKVSSVTVAELEEGMPEDEL
                     RTVLVIVPDNQLSLAIGKKGQNARLAAKLTGFRVDIKSEGEVEEERRRDEEERAEGRQ
                     SLGDLPGVGPQLVDRLVGAGLHSPARVVEGGLSALQELPGIGEAKASAILAAAQAWVA
                     EHPEPPAGSAEADAPAVEDGGSEIEPTHLRS"
BASE COUNT         1274 a         3385 c         3227 g         1398 t
ORIGIN      
        1 ttgaccagcc ccatcgcgat acccgccacc ggcgccttga gcggggcccc cgcgtccatg
       61 agggccatcg aggcgccgca gacggtggcc atcgaggacg agccgttgga ctcgaggatg
      121 tccgagacca cccggaccgt gtaggggaac tcctcccggg tcggcatcac cgcttcgacc
      181 gcccgctgcg cgagcgcgcc gtggccgatc tcgcggcggc ccggagttcc gaaccgcttc
      241 acctcgcccg tcgagaagga cgggaagttg tagtgcagca tgaagcgccg gaaggcattg
      301 ccctcgaacg cctcgatctt ctgctcgtcg tccttggtgc cgagcgtggc cgagacgagg
      361 gcctgggtct caccgcgggt gaagagcgcg gagccgtggg cgcgcggcag gtacgccacc
      421 tcgctccaga tcgggcgcac ttccttcacg ctccggccgt ccacgcggat gcccttctcc
      481 acgatgagcc ggcgcacctc ggccttctcg accttctcga agtagccgcg ggccttcgcc
      541 cgcaccgcat cgtctcctcc gatggccgcg aacacctcct cgaagatggc gtcgaggccc
      601 tgggcgcggg cctgcttctc gtgcacggac agcgcctgga ccaccttggc ggaggccaga
      661 tccttcaccc gcgcctcgag cgccggatcg gcgcccgcct gggcgtcgaa ggcccagcgc
      721 ggcttggccg ccttggccac gaggtccttc tggatccgcg tcagctcctt gcagcgctcg
      781 tggccgaagg ccagggcctc gagcatctgc tcctcgctga cttccttgcc accggactcg
      841 accatcagga tggcgtcttc ggtgcccgcc atcacgagct cgagcgggga ggccgccacc
      901 tgcttgtagg tgggattggc caccagcttg ccgtccacca gcgccacccg gaccgccccg
      961 accggacctg agaacgggat cccggacagc gacatcgcgg ccgaggagcc gatcatcgcg
     1021 agcacgtccg ggtcgttgtc ggagtccgcg gagatgacca ggttgatgat ctggatctcg
     1081 ttgcggaagc ccttggggaa gagcgggcgg agcggacggt cgatgagccg gcaggtcagc
     1141 gtctccttct cgtcgggccg gccctcgcgc ttgaagaagc cgccggggat ccggccgccc
     1201 gcgtacgagc gctcgcgata ctccacggtg aggggcagga agtcccggcc ctcaagggcg
     1261 gacctcgacg cgacggcggt ggagagcacc atggtcccgc cgcagcggac gaccaccgcc
     1321 ccgtcggcct gcttcgcgac ctttccggtc tcgatggtga gcggcgtgtt gttgatctga
     1381 atctgcacag agtgaggtgt catacggtca gcgtcctcct actaggaatg ggcagctaga
     1441 atgggttgcg acttcgagag gggcccggcc gggccgggca aatgtgagat cgatccaatc
     1501 aggatccgcg atcacttgcg aatcccgagc ttttccgtga tagctcggta gcgctccgag
     1561 tccttcttgc gcaggtactc gatgagaccg cggcgcttgc cgatcagcat gagcaatccg
     1621 cgacgggagt ggtggtcctt cttgtgcagc ttgaagtgct cggtgagccc gttgatccgc
     1681 tcggagagca gcgcgatctg gacctcgggc gagccggtgt cgccctcgtg caccttgaac
     1741 tgctcgatga gacccttctt cttgtcctgg gacagtcgca tgccgagatc ctcccggggg
     1801 ctcccccccc aatcgccacg cccccagagc cccgaccgcg gctccgttgg gacgggttcg
     1861 tttggaccgc tcaaacccgc gttaagacga gcggagtgta ccacgcgggg gtgaggggtt
     1921 caagcgatca ccccgccggt tgcgcccgca acagccgccg cgcctccgcc atatcggccc
     1981 ggacctgcgc ggtgagggcc tccacgcccg ggaacttcat ctcggcccgg atccggacga
     2041 ggaagtcgag cgtgagcgcg cggtcgtaga ggtccccggc gaagtcgaac agataggcct
     2101 cgacccagta ctgcgactcc tcgaaggtcg gccggtaacc gatgttgacg accgcctcgg
     2161 cctgacgacc atcccagcgc gcgtgggccg cgtacacccc cgcggccagg gccagggggc
     2221 gatccggctt caggttcgcg gtgggaaacc cgagcgtgcg gccacggccg gcgccccgct
     2281 gcacggtccc cgtcagcgaa tacgggcgac ccagaagagc ccgcgcctgc tccacgtcgc
     2341 cgtcacgcag ggcctcgcgg atggcgctgg acgacacggt gagcccgttg acctggagcg
     2401 gcgggagcac gtgagtgacg aacccacggt ggacgcccag ctcccgcagg aggaccgcgg
     2461 tcccgcgtgc cccgcggccg aaggtgtgat tgaagcccac caccacctcc cgggcgccca
     2521 gggtgcgcac cagcgcctcg tccacgaagg tctcggcctc gaggcgcgag aagtcgagcg
     2581 tgaacgggat gaccagggtg gcgtcgaggc cgtgctccgc gatgcgggcc aggttctcct
     2641 cgagcgtcgc gatgggcgcc ggcgccttct ccggcctcag caccgcggtc gggtgcgggt
     2701 cgaaggtgca cgcgaccgcg gagacgccga gcgcgcgggc ccgcgccacc gcggtgtcga
     2761 ggatcttggc gtgagcgaga tggatgccgt cgaaggcgcc gaaggcggcc accgacggcc
     2821 gcagctcagg cgggaaggac tgaaggtcgc ggaggatccg catggaggag ccgttcgggc
     2881 ttcacccggt cgccgagccc ctgccctact ccgagcaggg cgccgtcggg gccgtacacg
     2941 cggagcaggc cgccgacgcg ctcaccggtc gccaccgcct gcccgtgcgc gaacgcgcgg
     3001 gccccgtccg cgtcgagccg caccggcgcc agcgcggcga gcgcggtgtc gggcggcagg
     3061 agccgcggcc agagccgctc gccggagcgc gcctcgcgga cctcgtccca cgagacggcg
     3121 gcctcgagcg cgtagggtcc cacccgggtg cggacgagcg cggccagggc cgccccgcag
     3181 cccagcgtgg cgcccacgtc ggcagcgagg gtgcgcacgt acgtgccctt gccgcagcgg
     3241 atgcggatga cgaagtccgg gagcgcgacc gattcgagcc ggatcgagtg cacggtgacc
     3301 tctcgcggct cgcgctccac ctcgactccc tgccgggcca gctcgtagag gcgcttgcct
     3361 ccccggcgca gcgccgagta catcggcggc acctgccgga tgcggccgac gaacggtacc
     3421 agcgcggcct cgagcgcggg cccgtcgagc gcgggcaccg gccgcgtctc gagcaccgcg
     3481 cccgagaggt cctgggtctc ggtggtgacg cccaggcgga cggtggccag gtactccttg
     3541 tccagatcca ccaggtacgg tgtcagcttg gtcgcttccc cgaccaggat gggaagcacc
     3601 ccggtggcgg cgggatcgag cgtgcccccg tgcccgatgc gcggcgcccg gagcacgcgt
     3661 cgcaggtgcg ccaccacctg gaacgaggtg acccccgggc ccttctcgac cggcaggatg
     3721 ccggcgcggt ggcgatcgct cacgacgccg ggccgtcgag ggcctgtctc accgccgcga
     3781 gcagcgtggc cttcgcggac gcgagcggcc cgggcagcgt gcagcccgcc gcgttctcgt
     3841 gcccaccgcc gccgaactgg tgcgcgatgc ggttgaccgg cacgtcgccc ttgccccgga
     3901 ggcttgcctt cacgcggccg tcaccctcgg cgcggaggag caccgccacc ttgacgcccg
     3961 cgatggagcg cggataggtc acgagatcct cggcggccat gaaggcctcg gaggccgcac
     4021 cctcgggcac cgtgagcgcc gcgatgcgcc cgtcctcgct gacctcgacg cggctcagca
     4081 ggctacccag ggtgagcagc gcgtccgggg gacgccgctg gtagagccgg tccgtcacga
     4141 gcgcgggctc ggcgccttcc gcggtgagcg agcccgcgat ccggaaggtg cgcggcgtcg
     4201 tgttcgagta gcggaacgag ccggtgtcgg tgtggatcgc ggtgtagagg ccgagcgcgg
     4261 tgtcccggcc gacgggccag ccgagggcga cgagcaggtc gtagatcatc tccccgacgg
     4321 cggcggcgga ggggtcgatc cagttcagcg tcccgtagcg gcggttgtcg gggtgatggt
     4381 cgatgttgac cacccggctg cccggaccgc gagcgcccgc caggagcccc tccgtgcggc
     4441 cgtcgttcgg acagtcggtc agcacgatgg tgtcgaacgt cccgggggcg gtgcgccaca
     4501 cctgccagcg gtcggcaccc gggatgaaat cgagcacgtc gggcaccgga tcggggccgc
     4561 cgtaggtcac cgtccagccc gccttctcca tggcgagccc gaggcccagc agcgtgccga
     4621 gcacgtcacc gtccggatgg acgtgtccca gcatgagcgc cgagccgccc ggccgggcga
     4681 gcaggtccac gagctccgcc ggcggctccg gcgcgacgat ggtgacgctc acgcttcatc
     4741 ctccgacgac tcctcggatc gcttgagatc ggcgaggagc gcctggatgt gcgcggcgtg
     4801 ctccatggac cggtcggggc ggaacgagat ctcgggcgtc acccgcaggt ccaggtgacg
     4861 gcgcagccag ctccacacga agccccgcgc gctctcgagc gccttgaacg aggcggccca
     4921 ctgcgcgtcc ggtcccagca ccgacacgaa gaccttggcg tgcttgagat ccggggtcgt
     4981 gtccacctcg gtcacggtca cgaacccgag ccgcgggtcc ttgagctgcc gctgcacgac
     5041 catggagatc tcttccttga tgagctggtt gacgcgtacg agccgcttgc cttgcattta
     5101 gagtatttcc acctggatgt cggtcaccca gccgtcgacg tggtcctcga tgtagtccat
     5161 ggccttggag atgacttcgt tggcgtggcg ggagtcgccc gagacgcagg ccagagccag
     5221 ggtcgcgcgc tgccacacgt cgtgatggtc cacctcggcg accgagatct cgaagcggcg
     5281 gcgcagcgtg tccttcaacc ccttcagcgc gtggcgcttg cccttgagcg atccgacgtc
     5341 cggcaagtgc aactcgaccg tgcccaccgc cacccgcgct cccgacactg gccgagaccc
     5401 ccttcgacgc tagagcgtcc gggcgacctc ctccgtgctg aacgcctcga tgacgtcgtt
     5461 gggctgtacg cccttgatcc cttcgatgcc gacgccgcac tcgagcccct ggagcacctc
     5521 ccggacgtcg tccttgaagc gcttgagcga gctgatgacg ccctcgccca gcagggtctc
     5581 gccgcgcttg atgcgggcgc gggcgccgcg caccatcttc ccctcggtga ccgacgaccc
     5641 gtagatggtc ccgaccttgg tgatcgggaa gagctggcgc acctgggccc ggcccagcac
     5701 catctcgcgg atctcggggg ccagcatgcc ggacagcgcc gcccgcacgt cgttgatcgc
     5761 gtcgtagatc acgttgtagc tgcgcatgtc cacgccgttg gcctgggcct gcgtcgccgc
     5821 cttggggtcg gccttgacgt tgaagccgag cacgatggca ttggacgcgg acgcgagcat
     5881 cacgtccgac tcggtcaccg tgcccaccga gctgtggatg accttgagct tgacctcctc
     5941 ggtggagagc cgctcgagcg actcggtcag cgcttccacc gagccctgca cgtcggcctt
     6001 gagcacgagg cgcagctcct tgacctcgcc cgcctcgatc tgcttgtgca ggtcctcgag
     6061 ggtgatgcgg gtcgcactct tgcccttgag ccggtcgcgc tcgctgcgca cggtggcgat
     6121 ctgccgcgcc ttgcgctcgt cggagaccac cagcatcgtg tcccccgcct gcggcacgcc
     6181 ggagagcccg aggatctcga ccggatcgga cggccccgcg ctctgcacct tcttgccgcg
     6241 gtcgttgaag agggcgcgca cgcggccgga gtgcgagccg accaccaccg cgtcgccttc
     6301 cttgagcgtg cccgactgga tcagcgcggt ggccaccggg ccgcggcccc gatcgagccg
     6361 gccttccacg atcacgccgc gcccggcccg ggccggattg gcgcgcagct cgaggatctc
     6421 ggcctgcagc gcggtcatct cgaggagctg cgggatgccg tcgccgcgct tggccgaggt
     6481 gtggatgaag atggtctgcc caccccagtc ttccggcacg agcccgtgat tggacagctc
     6541 gcgcttgacc cggtcgggat cggagcccgg cttgtcgatc ttgttcaccg cgaccaggat
     6601 cggcacgttg gccgccttgg cgtggttgat cgcctccacg gtctggggca tcaccccgtc
     6661 gtcggccgcg acgaccagga ccaccatgtc ggtggcctgg gccccacggg cgcgcatcgc
     6721 ggtgaacgcc tcgtggcctg gggtgtcgag gaaggtgacc ttgccgtggc tcgtgtccac
     6781 ctggtacgcg ccgatgtgct gcgtgatccc gccgaactcg cgctcggcca ccttggtggt
     6841 gcggatcgcg tcgaggagcg aggtcttacc gtggtcgacg tggcccatca cggtcaccac
     6901 cggcggccgc gggcgcatct gcgccggatc ggactcttcc tcctcgagca cgtcgccctc
     6961 gagcgagcgc acctgcacgt cgcagtggaa cttgtcggcg acgagcttgg ccgcggtggg
     7021 atcgagcacc tcgttgaccg tggccatcac ccccaggtcg accagcgcct tgatcacctc
     7081 cgtggtcttg cggcgcatcg cggcggccag ctcccccacc gtgacggact ccggcagctt
     7141 gatcagctcg cgcttgatct cctgcggcgg ctcgggcggg ggcggcaggg ccccacccgg
     7201 tgctggcctg ggcgcggtcg gcaccacctc cggcggtgcg gggatcgcag ccgcagcggg
     7261 gggcggagcg gcgaccgcgg cggccgccgg acgcgcgggc gcagccgggc gcagcggagc
     7321 cgggggcgcc gcgggacgcg gcggtgagat caccgtacgc ggcggattca ggggaggcgg
     7381 caccggcgcg cgtcgcaccg ggggcgcggg cctcacctcg acgggcgggg ccgcggcggc
     7441 cggacggacg accggcgcgg gagccggcgg gctcgcaggt ctgggcggcg cgaccggcgg
     7501 gagcgggacg atctcggccg gcggcgcggg cgggacggcg gacggcgcgg gcctgaagat
     7561 ggtcgcggcc ggcttcaccg cgtccggcgc cgggaccagc tcgatgacct cgggcgtctc
     7621 ctcgaccgac ttggcggcgg tcttcttcgc ccccgccgcc ctcttcttcg gcgcggcggg
     7681 cgcggcaccc tcggccgcct tcggcttggc cgcgcgcttg ggcttcggct cggcgggcag
     7741 atcgcgtccg cgccccagct tgacccgcag ctcgttggcc gtcgcggcga ggagagggct
     7801 catggcccgc atgcccttgt ggcccatgcc ttcgagcgcc acaatcaaat ccttgctggt
     7861 gacgcccagc tccttcgcca gatcgatgac cttgacccgt gccgccacgc gcgctacctc
     7921 ctcggcctac tcgccagaat gaccaccgcg ggctcctgcg gaggacgact cgcacccttg
     7981 aacgcgtgcc ccagccgccc cgcctgcagc gccttctcca cgcactcggt cgtggcgcac
     8041 acatacgcgc cgcgccccgc cgcgcgccca tctcgatcta ccctcaccac gccgccttcg
     8101 tcccgcacga gccggatcaa ctccgccttc gcccgcacca cgcgacagcc gatgcaggtg
     8161 cgctgaggcg ggcgctgcgc tcgcttcagc ttctcaagtg ggtcggctcg atctccgatc
     8221 caccgtcctc gaccgcgggc gcgtccgcct cggcgctccc ggccggcggc tccggatgct
     8281 cggccaccca cgcctgggcg gccgccagga tcgcgctcgc cttggcctcg ccgatgcccg
     8341 ggagctcctg cagggcggat agcccgccct cgaccacgcg ggccggggaa tgcagcccgg
     8401 cacctaccag gcggtcaacc agctgggggc cgacgccggg caggtcgccc aggctctgcc
     8461 ggccctccgc ccgctcttcc tcatcgcggc gccgctcctc ctcgacctcg ccttcgctct
     8521 tgatatccac ccggaatccg gtgagcttcg cggccagccg cgcgttctgc cccttcttgc
     8581 cgatcgcgag ggagagctgg ttgtccggga cgatcacgag cacggtgcgc agctcgtcct
     8641 cgggcatgcc ctcctcgagc tcggcgaccg tgaccgacga caccttggcg ggcgacagcg
     8701 cgcgcgccac gaacaccgcc gagtcgggcg accactccac gatgtcgatc ttctcgccgc
     8761 gcagctcccg actgatgacc tggatgcgcg tgccccgcag gccgacgcac gccccgatcg
     8821 ggtccacgtc gcgcttggtg gacgacaccg ccaccttggc acgctcaccg ggctcgcggg
     8881 ccgccgcctt cacgaggacg atcccctccg caatctccgg aatctcggtc tcgaagagcc
     8941 ggaccaggaa accgggatgc gtgcgcgaga gcaggatctg cgggcccttg gccgtcttct
     9001 tgacctctag catgtaggcc cgcactcgat ccccggggtt gaagcggtcg ccgggaagct
     9061 gctcccgttc ggtgatgacc gcctccgcct tgcccagctc caccaccacg ttccgcttct
     9121 cgatgcggtg cacggtgccg cggacgatct tgccctcgcg gtcgatgaac tccgagtaca
     9181 ccgcgtcgcg ctcggcgtcg cgcacgcgct gcaggatgac ctgcttggcg gtctgggccg
     9241 cgatgcgccc gaactcctgg ggcgccagct cgatctccag ctcg
//
LOCUS       DASUFA010000203        21214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_622213, whole genome shotgun sequence.
ACCESSION   DASUFA010000203 DASUFA010000000
VERSION     DASUFA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 21214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21214
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622213"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFX87_07315"
     CDS             <1..703
                     /locus_tag="VFX87_07315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4994759.1"
                     /translation="KTPAEVRAMEGYGGSDDWIVMPLQCVTQWDSLAHIFYEGKMYNG
                     YDAALVTSSGAARNSIDKTKSRLAGRGVLLDVARQKGVTALEPGYAITVGDLEATAAA
                     EGVEVLPGDILLVRTGHMSRYLDRNDWRHYDLDPFPGVSVHTASWLHARQIAAIASDN
                     YAVEVRPSEIAGFRNPFHVVAIPNMGLTLGEIFFLEDLAADCAADGRYAFLLVAPPLP
                     VTRAVGTPINPYALK"
     gene            732..1370
                     /locus_tag="VFX87_07320"
     CDS             732..1370
                     /locus_tag="VFX87_07320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994760.1"
                     /translation="MTVDRDQAGQKALEFSEKLWKHGDFWEFETSEYERARFAQTIQV
                     LRGARFPRGVELGCGAGYFTRLLAPHVDQLVAYDISPTAIERARAAVGPDSAIDFRAG
                     NAMDYGWRAEGPWDLIVMNDTICYLGWLYSFFDIAWFASEVHEAIRPGGRFLMANTMD
                     KQGYDKLLLPHITRGYRDLVLNVGFALDREETIQGTKNGVEFATLISLFARR"
     gene            complement(1389..2093)
                     /locus_tag="VFX87_07325"
     CDS             complement(1389..2093)
                     /locus_tag="VFX87_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX4994761.1"
                     /translation="MKFLIINADDFGYGSGVNRAIALLHDQGVVTSAGLMVNTPGTEE
                     AVALAATRPRLSLGLHVNFTNEAQRLVEFDDPEVCRRELRGQFDRFVALTGRPPTHLD
                     SHQHVHRRPHCQPSFLELALECGLPLRDRPPVTFKGGFYGQWEYGVSQREKVSFEALT
                     SIVSTELHGGIYELAVHPGYLDPAADYVYHQDREWELATLSDPRLPALLHDNAIELIS
                     YHQLGRAVACLEGSAA"
     gene            complement(2113..3288)
                     /locus_tag="VFX87_07330"
     CDS             complement(2113..3288)
                     /locus_tag="VFX87_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994762.1"
                     /translation="MGRRRVLVVLGMMGRCPFGGQTWLYLNWLRGFQRLGHEVWYVED
                     DTTWPYDPVANTITEDPRYATRYLHRTLSAIGLGDRWAYRPLYRGGDECYGLPVGGLR
                     DLYGRCDALINLCGATVLNEEHALAARRVYIQTDPVTNELEVATGQLKTRTALASHDT
                     IVTYGENYRAPDCGVPLPPEITYLKTRQPVDLDLWPMAFDPAARSYTTIGNWKQPGHD
                     VTWRGETYCWSKHHEFLRFLDLPRRRPRQRFELSINIDDDADRARLRAHGWDVTSPLA
                     ASLDPWTYQAFFRTSRAEWTVAKDQNVRLRSGWFSERAACYLATGKPVITQSTGFENV
                     LPTGTGLFAFRDMDEIACAIETIESDYAAACRSARAVAEECLEATRVCRKLVEDIGL"
     gene            complement(3290..5032)
                     /locus_tag="VFX87_07335"
     CDS             complement(3290..5032)
                     /locus_tag="VFX87_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994763.1"
                     /translation="MALGGGLSALGIALDLAKPLPLAIVLDSILSNKPPPALLGPWFA
                     QLEPMAQLGLAAAAIVLIAVCRGMITMLSNYLTIDVGQRMVNDLRIAIWSHLQKLSLR
                     FHNRQQTGDLLFRVMADTYAIQGIVMNGALPLVSAALMLIGMFVIMLRYDWMLACVAL
                     VVSPPLYLAIRWLTTRIHGHATAAREAESDLYSRAETAIGAVKLVQAYGREDRVVQDF
                     RAGSERSLALSLRLYGTETLFVLVVESLLAAGTAAIVWLGALRVMSGALTIGELTVFL
                     SYLRDLYQPILSLSQNLAEINSARVGLERVFSVLDAELDVQDAPDAQPLPPITGEIRF
                     ENVSFAYDDGRQVLRDIDLRIAAGEHVAIVGRTGAGKSTLAGLVLRFFDPQRGRVTVD
                     GHDLRRVSLASLRRQVTLMLQEPIIFRATAFENIAWGSRSPDPAKVREAARRTEAESF
                     IEELPQGYDTILGEHGSTLSGGQRQRLALARALLRDTPISILDEPTSALDVATEAQVW
                     RNLEERLRGRTAIVIAHRLSTARRCDRILVIDDGAIIEQGPHDELMARHGAYFDLWQR
                     HGAESAAAAVPEDR"
     gene            complement(5215..5547)
                     /locus_tag="VFX87_07340"
     CDS             complement(5215..5547)
                     /locus_tag="VFX87_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994764.1"
                     /translation="MTRPALFVVKATIAPELEAAFNHWYDTVRSKEAAQVPGCLGMRR
                     YAALPLESEHAGSEPWQYMVCYEFDSEVSLQAFARSDTLRAMTRDYNARFGGAGDRAR
                     LAYRQIYP"
     gene            5649..6917
                     /locus_tag="VFX87_07345"
     CDS             5649..6917
                     /locus_tag="VFX87_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4994765.1"
                     /translation="MSQTPPPEGIGAPLAREGRSWPELARTLRDLKRDDLDWRRGRHA
                     AFVWHADDAVEEVAREAYALFMTENGLGLRVFPSLRRMEADVVGMVRHLLGGDAAASG
                     HLTSGGTESIFLATHAARQWARQQRPEVTEPEIVAAWSAHPAVNKAAHYLGMRVLRVP
                     VGAGFRADVAAMAAAITPRTVMLYGSAPTYSLGVIDPIAELADLARARGLWLHVDACV
                     GGILGPFVRALGHPVPEFGLALPGVTSVSADLHKSGYTAKGASVVLFRTAEHQAAGRY
                     DFDDWPTGLYSVNTFTGTRPGGAVAAAWAVMHFLGEAGYRRIAATVMEAKARLVKGLA
                     RIGAGLHVWGEPELWGVGFGAEDHDIFTIADRMTARGWSVGRIREPRGIHLMVTPVHA
                     PIIDEYVADLAHSVNEARAVTGPSPTRAVY"
     gene            6918..7718
                     /locus_tag="VFX87_07350"
     CDS             6918..7718
                     /locus_tag="VFX87_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994766.1"
                     /translation="MRAGIALALAALLLLTGCAMWIDRFVFFPDRRVGPPPAGVAERW
                     IATPDGLRLHAWSVPPRDDGPVLLWSHGNAGNIDSRRALLAALAARGLGVLAYDYRGY
                     GRSEGSPSEMGVYLDATAAFDDAVTRGTDPATIVCFGESLGGAVSIEVATRRRCGAVI
                     TVSAFTRLADVARRHYGPLGSLAGNRFDSLARLPRVAVPVLVAHGDQDEIVPFELGER
                     LFAAASEPKRFFRIAGADHNDALGRPALLDTIAEFAREAVGRREPGGA"
     gene            7806..8114
                     /locus_tag="VFX87_07355"
     CDS             7806..8114
                     /locus_tag="VFX87_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994767.1"
                     /translation="MSDLLNNAREFRQKLHRLEVLLEIEKGGRTRSWFAGKAPQAVER
                     AAAIKRLTQTGLSEAAAAPAHFRLTPEGREFLRDVRTKVGAGGALDWTHADEIDFSKL
                     "
     gene            complement(8133..9329)
                     /locus_tag="VFX87_07360"
     CDS             complement(8133..9329)
                     /locus_tag="VFX87_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX4994768.1"
                     /translation="MGPPSLRQVVSDFGGVYAVNALVAFIFAASGPVAIILTVGTRGG
                     LSAADLSSWLFGAFFVNGLISIAFCWIYRQPLVLFWSIPGTVLVGPALGHLTFPEVIG
                     AFLASGALVLALGLSGWVRRAMEAVPMPIVMGMVAGVFLRFGLDLVGALHREFWIAGS
                     MTAVFLAVTARPALARRLPPLIGAMAVGIVMIAGLGGFHPEAGGAVGLARPTLHAPAF
                     SWAAMVELVVPLAVTVLVVQNGQGIAVLRAAGHQPPIDAITVACGAGSLLTGFLGTVP
                     TCLTGPVNAIISGSGEARRQYTAGIAVGVLGCAFGLLSPTVTGLMLATPGAFIATVAG
                     LAMLRVLQSAFALAFRDRCALGALVAFLVTVADIQLAGIGAPFWGLLFGVAVTLLLER
                     GDLAVG"
     gene            complement(9345..10913)
                     /locus_tag="VFX87_07365"
     CDS             complement(9345..10913)
                     /locus_tag="VFX87_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994769.1"
                     /translation="MAAIDTALLRQMDDYILDGKFREATDAYCRLVAADPAAAAPVTT
                     HAMATAAPYLHVPAHHKLLPNGEFRNVNYDHTILGIRAGMRLMPWLSERERYLTLAQA
                     IWYVPQGLDVWAQLECGFPGHYAREQERCAEEVLQSNKQLHRHFEDLAPLCEGSVDER
                     FQRMFHAIVDGDKATAYRLFLGLAAQPELRERVRNEVLFAAIIDQQEFNSFRRVRHIG
                     HKAIRTRSMFDIADWVGWDEAHPYFYIAVPDLCNAPIFHSLYDHASFMLGITFKGAQY
                     RLHETNTAPLTEDEQDRLIGAVLAGDPVSVTHEITALLRRGRALTGIADMVMIAHATH
                     CVERLRTAIAYTVPMHSFDYANVVNYWLRNFKHTHQAKAVYLSAWFVTDTIREIDWYP
                     DDPAVAKPDYAPFRDWAEGLSASEALAELEGTIASQDPSRSVALVRAYQQRTGDRDAL
                     IRTLVHCAGKFQGDAHIFRNARSVIEEYYHNSASPRRKDVLFQAWAHYLSFYKKRSLS
                     TECYELYHHHFGTR"
     gene            11058..12242
                     /locus_tag="VFX87_07370"
     CDS             11058..12242
                     /locus_tag="VFX87_07370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4994770.1"
                     /translation="MTTTRTAEVVIIGGGVTGASTAFHLTRRGVADVIVVDKGAIASG
                     GTGKSSACVRQHYSTPETCRMIRYSLDFFQRFAEHTGGASCGFRHTGYLLGVDAKMRA
                     PMEASVALQRSVGIDTRLVSPAEMREIEPRLRADDLVAGCYEPGSGYCNPVETAQGFA
                     RAARAAGARILEDTAVVGLLVDGDRVRGVRTSAGDIHAPVVVNAAGLWSARVGAMAGV
                     ELPIRVCRHKISIVAWPEADRRPHPMVYDFVTNIYTRPEMGEHILVGGLDAEESHDTA
                     DPDAYREGVSLDESTEALARVSHRFPALADGHIARGYAGCFDVSPDWHPILDRVGPEG
                     YHVAAGFSGHGFKLSPAVGHMMATLIMEGPGGHPDLPTFRLARFAEGKPVRGTYGDWL
                     MC"
     gene            12236..13450
                     /locus_tag="VFX87_07375"
     CDS             12236..13450
                     /locus_tag="VFX87_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994771.1"
                     /translation="MLARSRPRHPWIVVGAVWATLAIASGLYFSFPIFFVALLDEFGW
                     SRGATAAAFSISSIVQGVLSPVVGTLVDRLGPRRVMLGGAGVLGLGCALSSRIDSLWS
                     LYLIVGVLTAAGACAVSWVPTGALIARWFSEGRGTAMGVAFSGMGAGVLTVGPFAQWL
                     IAGSGWRAAYLALGLGTLAVLLPLIWLGVHDGPAVPASARGTAPGALKWSAEIGDALR
                     TRAFWALFFAYLCTPLAVFTVVTHQVAFAVDHGFPRLFVASIFGLTGLLSVVGRIGFG
                     IAADRIGRATSATISYGCTAVGTLCLLGLEIWPLGAALYAYAFFFGLGFGARGPIITA
                     MASQIFPGRFGAIYGFLSVGNGIGGGVAPWFAGFVHDVTGSYRIAFLIAVGFCAVGAT
                     CFWLARPPRDAL"
     gene            13459..14253
                     /locus_tag="VFX87_07380"
     CDS             13459..14253
                     /locus_tag="VFX87_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994772.1"
                     /translation="MRLPGRVAAITGGALGIGRATALTFAEEGATVALGDVQLEAAET
                     VAQEIRARGGKAIAVGVDVGDAPAVQAFVDRVVAEFGRLDILFANAGIAYSAPFLEHP
                     EAQWHRVLRVNLTGVFLCCQAAARQMVKQGGGRIITTASINGFRGIENLVGYNAAKAG
                     VIELTRTMAVELAQHRIAVNAIAPAQIDTRLTRSLPEAARRRRVERIPMARFGEPEEV
                     ARVALFLASDDASYVTGHTLAVDGGYLAGGLWSGAPGVRTPGVTPG"
     gene            14301..15506
                     /locus_tag="VFX87_07385"
     CDS             14301..15506
                     /locus_tag="VFX87_07385"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994773.1"
                     /translation="MDHALSDVTVIDLGQVIAMPFCTMLLADLGARVIKVESPERGRE
                     RVALGVKRRRDGIEERVPAAQYRDRNKLGVTLELKTPAGLELFRELVEHADVVTENFS
                     VGTMERLGLGYEELRRINPGLVYASITAFGQTGPYAPQRGYDMLAQAISGYMSITGYP
                     DGPPTRSGQSISDYYAGMLCAFSIVSALHYRQRTGRGQRIDLALLDSLLVALDNLGER
                     YTVGGEILTRAGNVSFAGSATGIYATTDGHVAIAAAASNAVWARFCAVIGRDELTRAP
                     EFATAAARRDRRDEIAAIIQRWTGARGRAEVVRTLATAGVPAAPVNNVAEMVADPQVR
                     AREMFVELDHPVYGPLKTTGTPLKLSETPGRVRWLAPMPGEHNEEVFMGVLGHSPEEL
                     ERWRAEGVI"
     gene            15577..16743
                     /locus_tag="VFX87_07390"
     CDS             15577..16743
                     /locus_tag="VFX87_07390"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4994774.1"
                     /translation="MTSPFVPGPAVRIAGAPTGPLAGLTFAAKDLFDVAGHPTGGGNP
                     DWARQYPVPTRHAWAVQRLLDAGATLIGKTITDEVSLGILGENPFDGTPLNPKAPDRV
                     PGGSSSGSASAVAQGLCDTALGTDTGGSVRVPASFCGLHGIRPTHGRLDLTGMLPQAP
                     SSDTAGWFARDAQTFARVSEVLLGEPIPPARPARLVVAQDAFGLADEETAAALRPMVR
                     RLGELVPDVREEIMAPPGLTAWARAQRTLQPWEAWLTFKEWIDRDNPRMQWSVARNLA
                     LGSAIPESERQWAALVRAEARARLAALLPPGAILCLPTTPFPAPLKGLPLATLEPLRA
                     RITCLAAHGGLTGLPQVSLPGALVGGLPVGLSILGPRGSDAMLVAVATAMEAAR"
     gene            16740..17144
                     /gene="hpxZ"
                     /locus_tag="VFX87_07395"
     CDS             16740..17144
                     /gene="hpxZ"
                     /locus_tag="VFX87_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HEX4994775.1"
                     /translation="MTDLTPNIPEVVAEVGALFERYEQALIDKTVEVLDATFWDSPHT
                     IRYALHENGYGFAAIHAHRVARPPGPGIKERRLRLEILTLGRDLATVNLEFKVRGRDL
                     IGRQSQTWVRFPDLGWKVIGAHVSTMNAAPIW"
     gene            17227..18177
                     /locus_tag="VFX87_07400"
     CDS             17227..18177
                     /locus_tag="VFX87_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4994776.1"
                     /translation="MRKAIGLAVLLLVLSAAFGIGLAAYPDRPVKLIVPWAAGGDTDN
                     IFRPFVPALQKALGGTVVIANVAGASGTVGAREAKNAPPDGYTLYAVHDYIHSTYYTG
                     VSDVSYTDFEPVCLLSSTPSVLTASPKTKWSSWQELLGDAKARPGQISVGATLGSTSH
                     FFPALIEKAAGIKFKYVSYEGLAPRMNAILGGHIDLTDSNLTQKGKVDAGQLRFLAIA
                     TEKRSPEIPSVPTLKELGVNVVFSVERGIMAPKGTAADVLAKLEGACVQATKDSAFAE
                     SMKKQGTEVKYLDRKAYAEYLAKLDTLNKDLARDLGLLKR"
     gene            18185..18676
                     /locus_tag="VFX87_07405"
     CDS             18185..18676
                     /locus_tag="VFX87_07405"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4994777.1"
                     /translation="MLGRDGIAGLVCLAGSLGLLALTRGLPRSALVPIGAGFYPRIVL
                     VVTALFSALLIVSDVRARRHHPVAAPEAGRRAPRAASRNYRLVALTFIVFTAYVFALP
                     VLGYRAATVCFVLALQLLLQPAGRRPWVRIVAVALVTAVITHLVFESYLSVLLPRGRW
                     TGF"
     gene            18680..20206
                     /locus_tag="VFX87_07410"
     CDS             18680..20206
                     /locus_tag="VFX87_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4994778.1"
                     /translation="MFELLAAGIVNVAQLKYLIPLLVGTLGGVVGGALPGVTITMTII
                     LVLPFTYGLDPLQGLAAMTGVYVGGSAGGLITACLIGIPGTPSAIATTFDGFPMARKG
                     EPGRAVWLGTWASFWGGILGGLFLIGATAPLAAFALEFGPWEYFSLFIFALSMVAGLT
                     EASLLKGLLSGAIGLVVTVIGSDPIMAVPRLTMGSEFLRGGFQFLPILIGVFAFAQIM
                     ADVEKMGRRGETVQAVFAPSLAVSHPKIIWEILSRPFLLLWSTVIGILIGVLPAIGGS
                     AANIMAYDQAKKFSRHPERFGTGTPEGIIASESSNNANVGGSLVTIMAFGIPGDAVTA
                     VMLGAMIIHGIQPGPLFISQNPDLAYGIFAAYILAHPIMVLIIAAGARFFLHIVSVPK
                     AVLIPVVLVLCVIGAYALNNIMENVYVLLLFGLLGYAFVKLGFPLAPLILGVILGDQI
                     EVNLVRAIMTDPDPWLFLTRPISGALIALSAASVLVALWQHRRAHTRMGPPPEQAVDF
                     "
     gene            20222..20419
                     /locus_tag="VFX87_07415"
     CDS             20222..20419
                     /locus_tag="VFX87_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994779.1"
                     /translation="MIRGSAAATGDIVHRAGGATSIFEGNRIERCFRDVHTATPHGAL
                     APRTLELVGQARLGVDSQGLL"
     gene            20488..>21214
                     /locus_tag="VFX87_07420"
     CDS             20488..>21214
                     /locus_tag="VFX87_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4994780.1"
                     /translation="MLQRQVGEVIINRVIESERPDFDAAGFFPKITPEQWAPYRQRLA
                     GWAMDTGVNGLVFPMQSFLLRTRHHTIVVDTCVGDHKERARPTWNMTSSGEYLKRFAE
                     TGVRPEQVDFVMTTHFHTDHVGWNTRWDNGRWVPTFPNAKYVMSEKEWTYWSAMHKET
                     PQNQIADSVIPIVEAGRAQMVRNDFALDDEVRFESTAGHTPDHMSVHVQSNGQHAVIT
                     GDLIHSPVQLQETAWVPRPDFDPA"
BASE COUNT         2983 a         7605 c         7465 g         3161 t
ORIGIN      
        1 gaagaccccc gcggaggtcc gggcgatgga gggctacggc ggctccgacg actggatcgt
       61 gatgccgctg cagtgcgtga cccagtggga cagcctggcc cacatcttct acgagggcaa
      121 gatgtacaac ggctacgacg ccgcgctggt caccagcagc ggcgcggccc ggaacagcat
      181 cgacaagacc aagagccggc tggccggccg cggcgtgctg ctcgacgtgg cccggcagaa
      241 gggcgtgacg gcgctcgagc ccggctacgc gatcacggtg ggcgacctgg aggcgaccgc
      301 ggccgccgag ggcgtcgagg tgctgccggg cgacatcctc cttgtccgca ccgggcacat
      361 gtcccgctat ctcgaccgga acgactggcg gcactacgac ctcgatccgt tccccggcgt
      421 ctcggtgcac accgcgtcgt ggctgcacgc ccggcagatc gcggcgatcg cgagcgacaa
      481 ctacgcggtg gaggtccgcc cctcggagat cgcgggcttc cgcaatccct tccacgtggt
      541 cgcgatcccc aacatgggcc tcacgctcgg cgagatcttc ttcctggagg acctggccgc
      601 cgactgcgcg gccgacggcc gttacgcctt cctgctcgtg gcgccgccgc tgccggtgac
      661 gcgcgcggtg ggaactccca tcaatcccta cgcgctgaaa tagccccgga ccgaccggag
      721 gagagtcgcc gatgaccgtc gatcgagacc aggccggcca gaaggcgctg gagttctccg
      781 agaagctgtg gaagcacggc gacttctggg aattcgagac ctcggagtac gagcgcgccc
      841 gcttcgcgca aacgatccag gtgctccggg gcgcgcgctt cccgcggggc gtcgagctgg
      901 gctgcggggc gggctacttc acccggctcc tcgccccgca cgtggatcag ctcgtcgcct
      961 acgatatctc tccgaccgcg atcgagcgcg cccgcgccgc ggtcggcccc gacagcgcga
     1021 tcgacttccg cgcgggcaac gccatggact acgggtggcg cgccgagggg ccgtgggatc
     1081 tgatcgtgat gaacgacacc atctgctacc tcgggtggct gtactcgttc ttcgacatcg
     1141 cgtggttcgc gtccgaggtc cacgaggcga tccggccggg cggccggttc ctcatggcca
     1201 acaccatgga caagcagggg tacgacaagc tcctgctgcc ccacatcacc cggggctacc
     1261 gcgacctcgt cctcaacgtc ggcttcgcgc tcgatcggga ggagacgatc cagggcacga
     1321 agaacggggt ggagttcgcg accctgatct cgctcttcgc ccggcgctga ggcgccccgc
     1381 gcgcccgctc aggccgcgct gccctcgagg cacgcgaccg cgcgcccgag ctggtggtag
     1441 ctgatgagct cgatcgcgtt gtcgtggagc agcgcgggca agcgcggatc ggacagggtc
     1501 gcgagctccc actcccggtc ctggtggtac acgtagtccg cggccgggtc gaggtagccg
     1561 gggtgcacgg ccagctcgta gatgccgccg tggagctcgg tggacacgat gctggtgagg
     1621 gcctcgaagc tcaccttctc ccgctgggac actccgtact cccactgacc gtagaagccg
     1681 cccttgaagg tgacgggggg ccggtcgcga aggggcaggc cgcactccag agccagctcg
     1741 aggaagctcg gctggcagtg cggccgccgg tgaacatgct ggtgggagtc gagatgggtc
     1801 gggggccggc cggtcagcgc gacgaagcgg tcgaactgcc cgcgcagctc gcgccggcac
     1861 acctccgggt cgtcgaactc cactaggcgc tgggcctcgt tggtgaagtt cacgtgcagg
     1921 ccgagggaaa gccggggccg cgtcgccgcg agggcgaccg cctcctcggt ccccggcgtg
     1981 ttcaccatga gcccggccga agtcaccacc ccctggtcgt ggaggagggc gatggcccgg
     2041 ttcacgccgg agccgtaccc gaaatcatcg gcgttgatga tcaggaattt catccgtgtc
     2101 aggccctccc ggctacaagc cgatatcctc gacgagcttc cggcatacgc gcgtggcctc
     2161 caggcactcc tccgcgaccg cccgcgccga gcggcacgcg gcggcgtagt cggactcgat
     2221 ggtctcgatg gcgcaggcga tctcgtccat gtcgcgaaac gcgaacaggc cggtgccggt
     2281 gggcagcacg ttctcgaacc cggtgctctg ggtgatcacc ggcttgccgg tggccaggta
     2341 acaggccgcg cgctcgctga accagccgct ccggagccgc acgttctggt ccttggccac
     2401 cgtccactcc gcccgcgacg tccggaagaa cgcctggtac gtccacgggt cgagcgaggc
     2461 ggccagcggg ctggtgacat cccagccgtg ggcccgcaat cgggcgcggt ccgcgtcgtc
     2521 atcgatgttg atcgagagct cgaagcgctg ccggggccgg cgccggggga gatcgaggaa
     2581 cctcaggaac tcgtggtgct tgctccagca ataggtctcg ccgcgccagg tcacgtcgtg
     2641 gccgggctgc ttccagttgc cgatggtggt gtagctccgc gcggccgggt cgaacgccat
     2701 cggccagagg tcgagatcca ccggctgacg cgtcttgagg taggtgatct cgggcggcag
     2761 tggcacgccg caatcgggcg cccggtagtt ctccccgtag gtcacgatcg tgtcgtggga
     2821 ggccagcgcc gtgcgtgtct tcagctgccc ggtcgcgact tccagctcgt tggtgaccgg
     2881 atcggtctgg atgtagacgc gccgcgcagc cagggcgtgc tcctcgttga gcacggtggc
     2941 cccgcacagg ttgatcagcg cgtcacaacg cccgtagaga tcgcgcaggc cgccgaccgg
     3001 gagcccgtag cactcgtccc cgccgcgata gagcggccgg taggcccagc ggtcgcccag
     3061 cccgatggcc gagagcgtcc ggtgcaggta gcgggtggcg taccgcggat cctcggtgat
     3121 ggtgttggcg accgggtcgt agggccacgt ggtgtcgtcc tccacgtacc agacctcgtg
     3181 cccgagccgc tggaagccgc gcagccagtt gagatagagc caggtctgcc cgccgaaggg
     3241 acagcggccc atcatcccga gcaccacgag cacgcgccgg cggcccacct taccggtcct
     3301 ccgggacggc ggcggccgcg gactcggccc cgtgccgctg ccagagatcg aagtacgccc
     3361 cgtgccgggc catcagctcg tcgtggggac cctgctcgat gatggcgccg tcgtcgatca
     3421 ccaggatgcg gtcgcagcgc cgggcggtgc tcagccggtg cgcgatcacg atcgcggtcc
     3481 gcccgcgcag ccgctcctcc aggttgcgcc agacctgagc ctcggtggcc acgtccagcg
     3541 cgctggtcgg ctcgtccagg atcgagatcg gggtgtcgcg gaggagcgcc cgcgccaggg
     3601 ccaggcgctg gcgctgcccg cccgagaggg tggagccgtg ctcgcccagg atcgtgtcgt
     3661 agccctgggg cagctcctcg atgaaggact cggcctcggt gcggcgggcg gcctcccgca
     3721 ccttcgcggg atccgggctc cgcgagcccc acgcgatgtt ctcgaacgcg gtggcccgga
     3781 agatgatcgg ctcctgcagc atcagggtga cctggcgccg gagcgacgcc aggctgaccc
     3841 ggcgcaggtc gtggccgtcg acggtgacgc gcccgcgctg gggatcgaag aagcgcagca
     3901 cgagcccggc cagggtgctc ttgcccgccc cggtccggcc cacgatggcc acgtgctcgc
     3961 cggcggcaat gcgcaggtcg atgtcgcgga gcacctgccg gccgtcgtcg taggcgaagc
     4021 tcacgttctc gaaccggatc tcgccggtga tcggcggcag cggctgagcg tcgggcgcgt
     4081 cctggacgtc gagctccgcg tcgagcaccg agaagacgcg ctccagaccc acgcgggccg
     4141 agttgatctc ggcgaggttc tggctcaggc tgaggatggg ctggtagagg tcgcgcaggt
     4201 aggagaggaa cacggtcagc tcgcctatgg tcagggcgcc gctcatcacc cggagcgcgc
     4261 cgagccacac gatcgccgcg gtgcccgcgg ccagcaggga ctccaccacc agcacgaaca
     4321 gggtctccgt cccgtacagg cggagcgaca gggccaggct ccgctcgctg ccggcccgga
     4381 agtcctggac cacccggtcc tcgcgcccgt aggcctgcac cagcttcacg gccccgatgg
     4441 cggtctccgc gcggctgtag aggtcgctct ccgcctccct ggcggcggtg gcgtggccgt
     4501 ggatccgggt ggtcagccag cggatcgcga ggtagagcgg cgggctcacc accagggcca
     4561 cgcaggccag catccagtcg tagcgcagca tgatcacgaa catcccgatc agcatgagcg
     4621 ccgcgctgac cagcggcagc gccccgttca tcacgatgcc ctggatcgcg taggtgtcgg
     4681 ccatcacccg gaagagcagg tcgccggtct gctgccggtt gtggaaccgg agcgagagct
     4741 tttgcaggtg agaccagatc gcgatgcgca ggtcgttgac catacgctgg cccacgtcga
     4801 tcgtgaggta gttggacagc atggtgatca tgccgcggca gaccgcgatc agcacgatcg
     4861 ccgcggcggc gaggccgagc tgggccatcg gctcgagctg cgcgaaccac ggccccagca
     4921 gggccggggg cggcttgttg ctgaggatgc tgtcgaggac gatggccagc ggcagcggct
     4981 tggcgaggtc gagggcgatc ccgagggcgg agagtccgcc tcctagcgcc atgggcgccc
     5041 ggtgggggcg cgcgtaccgg gcgatgcgtg ggagcagggc caggaggccg cgcgatccgg
     5101 ctccgccggt cacaagagcg ccctgcgctg attgtatctc attggtggtg taaccctgcg
     5161 gcggggaacg gcgttccccg cccaagtcaa cggattctcg cacatcgaag acggctacgg
     5221 gtagatctgc cggtatgcca gtcgcgcgcg gtcccccgcc ccgccgaagc gggcattgta
     5281 gtcgcgggtc atggcccgca gcgtgtccga ccgggcgaag gcctgcagcg acacctcgga
     5341 gtcgaactcg tagcagacca tgtactgcca cggctcggag ccggcgtgct cgctctcgag
     5401 gggcagcgcc gcgtagcgtc gcatcccgag acagccgggg acctgggcgg cctccttcga
     5461 gcggaccgtg tcgtaccagt ggttgaaggc cgcctcgagc tcgggggcga tggtggcctt
     5521 gacgacgaag agcgcgggac gtgtcattcg atgagcctcc tcgggggcga tgagcctcct
     5581 cccgcgcacg gtaacaggtc gcggggacgt tgccgagcct cggatgtcgg ggtataacct
     5641 cgcccatcat gagccagaca cccccacctg aagggatcgg cgcaccgctc gcgcgtgaag
     5701 gccggagctg gcccgagctc gcgcggacgc tccgcgacct caagcgcgac gatctcgact
     5761 ggcgtcgggg ccggcacgcg gccttcgtct ggcacgccga cgacgcggtg gaggaggtgg
     5821 cccgcgaggc gtacgcgctc ttcatgaccg agaacggact cgggctgcgc gtgttcccca
     5881 gcctgcggcg catggaggcc gacgtcgtcg ggatggtccg tcacctcctc ggaggcgatg
     5941 ccgcggccag cggccacttg acctccggcg gcacggagag catcttcctc gccacccacg
     6001 cggcacgcca gtgggcgcgg caacaacgcc ccgaggtcac cgagcccgag atcgtggccg
     6061 cgtggtcggc gcatcccgcc gtgaacaagg cggcccacta cctcggcatg agggtgctcc
     6121 gcgtgccggt cggagccggc ttccgggccg acgtggccgc gatggccgcc gcgatcaccc
     6181 cgcgcaccgt catgctctac ggctcggcgc ccacctactc gctcggcgtc atcgatccca
     6241 tcgccgagct ggccgatctc gcgcgggcgc gcgggctgtg gctccacgtg gacgcctgcg
     6301 tgggcgggat cctcggccca ttcgtccgcg cgctgggtca cccggtgccg gagttcggcc
     6361 tggccctgcc cggcgtcacc tcggtgtcgg ccgatctcca caagtcgggc tacaccgcca
     6421 agggcgcctc ggtggtcctc ttccggaccg ccgagcacca ggcggcgggg cgctacgatt
     6481 tcgacgactg gcccaccggt ctctacagcg tcaacacctt caccggcacg cggcccggcg
     6541 gcgccgtcgc ggcggcgtgg gcggtgatgc acttcctggg cgaggcgggg taccgccgca
     6601 tcgcggccac cgtcatggag gccaaggcgc gcctcgtcaa gggcctcgcg cgcatcggag
     6661 cagggctgca cgtatggggc gagcccgagc tgtggggggt gggcttcggc gccgaggacc
     6721 acgacatctt caccatcgcc gatcggatga cggcccgggg ctggtcggtg gggcgcatcc
     6781 gcgagccgcg cgggatccac ctcatggtga ctccggtgca cgcgccgatc atcgacgagt
     6841 acgtggcgga cctggcccac tcggtgaacg aggcgcgggc ggtcaccggc ccgtcgccca
     6901 ctcgcgccgt ctactgagtg cgggccggga tcgccctcgc gctggccgcg ctcctcctgc
     6961 tgaccggctg cgccatgtgg atcgaccgct tcgtcttctt tcccgaccgg cgggtcgggc
     7021 cgccgcccgc cggcgtggcg gagcggtgga tcgccacccc ggacggcctg cggctgcacg
     7081 cctggtccgt ccccccgcgc gacgacggcc ccgtgctcct gtggtcgcac ggcaacgccg
     7141 ggaacatcga ctcgcgccgg gcgctgcttg ccgcgctggc cgcccgcggg ctcggggtgc
     7201 tcgcctacga ctaccgcggc tacgggcgca gcgagggctc gcccagcgag atgggtgtct
     7261 acctcgacgc gaccgccgcc ttcgacgacg cggtgacgcg tgggacggac ccggccacca
     7321 tcgtgtgctt cggcgagtcg ctcggagggg cggtctcgat cgaggtcgcc acccggcgcc
     7381 gctgcggagc ggtcatcacg gtctcggcgt tcacgcggct ggccgacgtc gcgcgccggc
     7441 actatggccc gctgggctcg ctcgcgggca accggttcga ctcgctggcc cgcctgccgc
     7501 gggtggcggt gccggttctc gtcgcgcacg gcgatcagga cgagatcgtc ccgttcgagc
     7561 tcggggagcg gctgttcgcg gcggcctcgg agcccaagcg cttcttccgc atcgccgggg
     7621 ccgaccacaa cgacgccctg ggccgccccg ccctgctcga caccatcgcg gagttcgccc
     7681 gggaggcggt ggggcggcgc gaaccgggcg gagcctagaa gccggtctcc aggctgacct
     7741 cgatctggtc gagcatgatg tgggctcctg gagtccgctt cccgatagtg ctaagctccg
     7801 gcccgatgag cgatcttctc aacaacgcgc gggagttccg gcagaagctc caccgcctcg
     7861 aggtgctgct ggagatcgag aaggggggac gaacccggag ctggtttgcg ggcaaggccc
     7921 cgcaggcggt ggagcgggcg gcggcgatca agaggctcac ccagaccggt ctcagcgagg
     7981 ccgcggcggc gcccgctcac ttccggctga ctcccgaagg ccgggaattc ctgagggacg
     8041 tccggaccaa ggtcggcgcc ggcggtgcgc tggactggac ccacgcggac gagatcgact
     8101 tctcgaagct ctgatcccgc cgcccccgcg ggctacccga cggccaggtc gccccgctcg
     8161 agcagcagcg tcaccgcgac cccgaacagc aagccccaga acggcgctcc gatgccggcg
     8221 agctggatgt cggccaccgt gacgaggaag gcgacgagcg cgccgagggc acaccgatcc
     8281 cggaacgcga gggcgaaggc cgactgcaac acccgcagca tcgccaggcc cgcgaccgtg
     8341 gcgatgaagg cgccgggcgt agccagcatg aggccggtga cggtcgggga caggagcccg
     8401 aaggcacagc cgaggacgcc gaccgcgatg cccgcggtgt actggcggcg cgcctctccg
     8461 ctccccgaga tgatggcgtt gaccgggccg gtgaggcagg tgggcaccgt gccgaggaac
     8521 cccgtcagca gggaccccgc cccgcacgcc accgtgatgg cgtcgatcgg cggctggtgc
     8581 ccggccgcgc gcagcacggc gatgccctgc ccgttctgca ccaccagcac cgtgaccgcg
     8641 agcggcacca ccagctcgac catcgccgcc caggagaacg ccggcgcatg gagggtgggc
     8701 cgcgcgaggc cgacggcgcc gccggcctcg ggatggaagc cgccgaggcc ggcgatcatg
     8761 acgatgccga cggccatcgc tccgatcagg ggcggcaggc ggcgggccag cgcgggccgc
     8821 gcggtcacgg ccaggaacac cgcggtcatc gatcccgcga tccagaattc acggtggagg
     8881 gcccccacga ggtcgagccc gaaccgcagg aatacccccg cgaccatccc catcacgatg
     8941 ggcatgggga ccgcctccat cgcgcgccgc acccagccgg agagcccgag cgccaggacg
     9001 agggcgccgc tcgccaggaa cgcgccgatg acctccggga aggtcaggtg cccgagcgcc
     9061 gggcccacca gcacggtgcc ggggatcgac cagaagagga cgagcggctg gcggtagatc
     9121 cagcagaagg cgatgctaat cagcccgttg acgaagaagg cgccgaagag ccaggaggac
     9181 agatcggcgg cgctcaggcc gccgcgggtc cccaccgtga ggatgatggc gaccggtccc
     9241 gaggccgcga agatgaacgc gaccagcgcg ttgacggcgt agacgccgcc gaagtccgag
     9301 acgacctgac ggagcgaggg cgggcccagc ggcggtcggc gcccctatcg cgtcccgaag
     9361 tgatggtggt acagctcgta gcactcggtc gacagcgacc gcttcttgta gaacgacagg
     9421 tagtgcgccc aggcctggaa caggacgtcc ttccgtcgcg ggctcgccga attgtgatag
     9481 tactcctcga tgaccgagcg ggcattgcgg aagatgtggg cgtcgccctg gaacttcccc
     9541 gcgcaatgga ccagcgtgcg gatcagcgcg tcgcggtccc ccgtccgctg ctggtaggcg
     9601 cgcaccaggg ccaccgagcg ggacgggtcc tggctggcga tggtcccctc cagctccgcc
     9661 agggcctcgg atgcggacag cccctcggcc cagtcgcgga acggtgcgta gtccggcttg
     9721 gccaccgcgg gatcgtcagg gtaccagtcg atctcgcgga tggtgtcggt gacgaaccac
     9781 gcgctcaggt acaccgcctt ggcctggtgc gtgtgcttga agttccgcag ccagtagttg
     9841 accacgttgg cgtagtcgaa ggagtgcatc ggcacggtgt aggcgatggc ggtgcgcagc
     9901 ctctccacgc agtgggtggc gtgggcgatc atcaccatgt cggcgatacc ggtcagcgcc
     9961 cttccccgcc gcaggagcgc ggtgatctcg tgggtcaccg agacggggtc gccggccagg
    10021 accgctccga tgagccgatc ctgctcgtcc tcggtcagcg gggcggtgtt ggtctcgtgc
    10081 agccggtact gggcgccctt gaaggtgatg ccgagcatga agctggcgtg gtcgtagagc
    10141 gagtggaaga tcggcgcgtt gcagaggtcg ggcaccgcga tgtagaagta cgggtgcgcc
    10201 tcgtcccagc ccacccagtc tgcgatgtcg aacatcgagc gcgtgcggat ggccttgtgc
    10261 ccgatgtgcc ggacccgccg gaacgagttg aactcctgct ggtcgatgat ggcggcgaag
    10321 aggacctcgt tgcgcacgcg ctcgcgcagc tcgggctggg cggcgagccc caggaacagc
    10381 cggtaggccg tggccttgtc gccgtcgacg atggcgtgga acatccgctg gaagcgctcg
    10441 tcgaccgagc cttcgcagag cggcgccagg tcctcgaagt ggcggtggag ctgcttgttg
    10501 ctctgcaaca cctcctccgc gcacctctcc tgctcgcgcg cgtagtggcc cgggaatccg
    10561 cattcgagct gcgcccacac gtccaggccc tggggcacgt accagatggc ctgggccagc
    10621 gtcaggtacc gctcgcgctc cgacagccag ggcatgaggc gcatgccggc ccggatgccc
    10681 aggatcgtgt ggtcgtagtt gacgttgcgg aactcgccgt tcggcagcag cttgtggtgc
    10741 gccggcacgt gaaggtaggg cgcggcggtg gccatcgcgt gggtggtgac cggtgcggcc
    10801 gcggcggggt cggcagccac cagccggcag tacgcgtcgg tcgcctcgcg aaacttgccg
    10861 tcgaggatat agtcgtccat ctgccggagg agcgcggtat cgatggcggc cattggtcag
    10921 ctccctttct ctcgttcggt cggacgcacc gcccggcgat gttagcgcag tccggtccgc
    10981 tccgccctag gtcgattgcc ctagggggac cgggccgtcc gttgcgcccg ggcgtcgcgc
    11041 cggttagcat cgcccccatg acgacgacgc gcaccgcgga ggtggtgatc atcgggggtg
    11101 gggtgaccgg cgccagcacc gcgtttcacc tgacccggcg cggcgtcgcc gacgtgatcg
    11161 tcgtcgacaa gggcgcgatc gcctccgggg gcaccgggaa gagctcggcg tgcgtgcggc
    11221 agcactactc gacacccgag acctgccgga tgatccggta ctcgctcgac ttcttccagc
    11281 gctttgccga gcacaccggc ggagcctcgt gcggcttccg gcacaccggc tacctgctcg
    11341 gggtggacgc caagatgcgc gcgcccatgg aggcctcggt cgcgctccag cgctcggtcg
    11401 gcatcgacac gcggctcgtc tcccccgccg agatgcgcga gatcgagccc cgtctgcgcg
    11461 cggacgatct ggtggccggc tgctacgagc cggggtcggg ctactgcaac cccgtcgaga
    11521 ccgcccaggg cttcgcgcgg gccgcgcgcg cggcgggcgc gcgcatcctg gaggacaccg
    11581 cggtggtcgg gctgctcgtg gacggcgacc gcgtccgcgg cgtccgcacc agtgcgggcg
    11641 acatccacgc ccccgtggtc gtcaacgcgg cgggcctgtg gagcgcccgg gtgggcgcca
    11701 tggccggcgt cgagctgccg atccgcgtgt gccgtcacaa gatcagcatc gtcgcctggc
    11761 ccgaggccga tcggcgcccg cacccgatgg tgtacgactt cgtcaccaac atctacaccc
    11821 gccccgagat gggcgagcac atcctggtgg gcgggctcga cgccgaggag tcccacgaca
    11881 cggccgaccc ggacgcctac cgggaaggcg tgagcctcga cgagtccacc gaggccctcg
    11941 cgcgtgtgag ccatcgcttt cccgcgctcg cggacggcca catcgcccgc ggctacgccg
    12001 gctgtttcga cgtcagcccg gactggcacc ccatcctcga ccgggtcggc ccggagggct
    12061 accacgtggc cgcgggcttc tccggccacg gcttcaagct gtccccggcc gtgggccaca
    12121 tgatggcgac gctcatcatg gaggggccag gcggccaccc cgatctgccc accttccgtc
    12181 tggctcggtt cgcggagggc aagccggtcc gaggaaccta cggcgactgg ctgatgtgct
    12241 agcgaggtcc cggccccggc atccctggat cgtcgtcggc gcggtctggg cgacgctcgc
    12301 gatcgcgtcc ggcctctact tctcgttccc gatcttcttc gtggcgctgc tcgacgagtt
    12361 cggctggtcg cgcggggcca ccgcggcggc gttctcgatc tcgtcgatcg tgcaaggggt
    12421 gctctccccg gtggtgggga cgctggtgga ccgcctgggg ccgcgccggg tgatgctcgg
    12481 cggggcgggc gtgctcgggc tggggtgcgc cctgtcgagc cggatcgact ccctgtggtc
    12541 gctgtacctg atcgtcggcg tgctgaccgc ggccggggcc tgcgcggtga gctgggtgcc
    12601 gacgggcgcg ctgatcgccc gatggttcag cgaggggcgc ggcaccgcga tgggcgtggc
    12661 gttctcgggc atgggcgccg gcgtgctgac ggtggggccg ttcgcccagt ggctgatcgc
    12721 cggatccggc tggcgcgccg cctatctcgc cctggggctg ggcacgctcg ccgtgctcct
    12781 tcccctcatc tggctgggcg tgcacgacgg gcccgcggtc cccgcgtcgg ctcgaggcac
    12841 cgccccggga gctctcaagt ggtccgcgga gatcggcgat gcgctgcgga cgcgggcgtt
    12901 ctgggcgctc ttcttcgcgt acctgtgcac gccactggcc gtcttcacgg tcgtcacgca
    12961 ccaggtggcc ttcgcggtgg accacggctt cccgcggctg ttcgtcgcct cgatcttcgg
    13021 gctcaccgga ctgctctcgg tcgtgggccg gatcggcttc ggcatcgccg ccgatcggat
    13081 cgggcgcgcc acgtcggcca ccatctccta cggctgcacc gcggtgggca cgctctgcct
    13141 gctcgggctc gagatctggc cgctcggggc ggcgctctac gcctacgcgt tcttcttcgg
    13201 cctgggtttc ggcgcccgcg gccccatcat caccgcgatg gcctcccaga tctttccggg
    13261 gcgcttcggc gccatctacg gcttcctcag cgtcggcaac ggcatcggcg gcggcgtcgc
    13321 cccgtggttc gccggcttcg tccacgacgt caccgggagc taccggatcg ccttcctgat
    13381 cgccgtcggc ttctgcgcgg tgggcgcgac gtgcttctgg ctcgcgcgcc ctccgcgcga
    13441 tgcgctatga tccggccgat gcgcctgccg ggtcgggtcg cggcaatcac cggaggagcg
    13501 ctcggcatcg ggcgcgcgac cgcgctcacc tttgccgagg agggcgcgac cgtcgccctc
    13561 ggcgacgtcc agctcgaggc cgccgagacg gtggcgcagg agatccgcgc gcgcggcggc
    13621 aaggcgatcg cggtcggcgt ggacgtgggc gacgccccgg ccgtccaggc cttcgtcgac
    13681 cgcgtggtcg ccgagttcgg ccggctcgac atcctcttcg ccaacgcggg gatcgcgtac
    13741 tcggcgccgt tcctggagca ccccgaggcc cagtggcacc gggtcctgcg cgtcaacctg
    13801 accggcgtct tcctctgctg ccaggccgcc gcgcgtcaga tggtcaagca gggcggcggc
    13861 cggatcatca ccaccgcgtc gatcaacggc ttccggggca tcgagaacct ggtcgggtac
    13921 aacgcggcca aggccggcgt catcgagctg acccgcacca tggcggtcga gctggcgcag
    13981 catcgcatcg cggtgaacgc gatcgccccc gcccagatcg acacccggct cacgcggagc
    14041 ctgcccgagg ccgcccgacg ccggcgggtg gagcgcatcc cgatggcgcg cttcggcgag
    14101 cccgaggagg tggcccgggt ggcgctcttc ctcgcctccg acgacgccag ctacgtcacc
    14161 ggccacacgc tcgcggtgga tggcggctac ctggcgggcg gcctctggtc cggcgcgccc
    14221 ggcgtccgga cgcccggcgt caccccgggc tagggctccg cgcccggcct cccgtccgcg
    14281 atcgagcccg ccgaggcgga atggatcacg ccctgagcga cgtcaccgtg atcgacctcg
    14341 gtcaggtcat cgcgatgccg ttctgcacga tgctcctcgc cgacctcggc gcgcgggtga
    14401 tcaaggtgga gtctccggag cgcgggcgcg agcgcgtggc gctcggcgtg aagcgccgcc
    14461 gcgacggcat cgaggagcgg gtgcccgccg cgcagtaccg cgatcgcaac aagctcgggg
    14521 tcaccctcga gctgaagaca ccggccggcc tcgagctgtt ccgcgagctg gtcgagcacg
    14581 cggacgtggt caccgaaaat ttcagcgtcg gcacgatgga acgtctcggc ctcggctacg
    14641 aggagctgcg ccgaatcaat cctgggctcg tctacgcctc gatcaccgcg ttcggccaga
    14701 ccgggcccta cgcgccccag cggggctacg acatgctcgc gcaggcgatc agcgggtaca
    14761 tgagcatcac cggctatccc gacgggccgc ccacccggtc cggccagtcc atctcggact
    14821 actacgcggg catgctatgc gccttcagca tcgtgtccgc gctgcactac cggcagcgca
    14881 ccggccgagg gcagcgcatc gacctggccc tgctcgacag cctcctggtg gcgctggaca
    14941 acctcgggga gcgctacacg gtgggcggcg agatcctcac ccgcgccggc aacgtctcgt
    15001 tcgccgggtc ggccaccggg atctatgcga ccaccgacgg ccacgtggcg atcgcggccg
    15061 ccgcgagcaa cgcggtgtgg gcccgcttct gcgcggtcat cggccgggac gagctgactc
    15121 gcgcccccga gttcgccacc gcggcggcgc ggcgcgaccg ccgcgacgag atcgccgcca
    15181 tcattcagcg gtggaccggg gcgcgcggca gggcggaggt cgtgcgcacc ctcgccacgg
    15241 ccggcgtccc ggccgcgccg gtgaacaacg tggcggagat ggtcgccgat ccccaggtgc
    15301 gggcgcgcga gatgttcgtc gagctcgacc atcccgtcta cggtcccctg aagacgaccg
    15361 gaacgcccct gaagctctcg gagacgcccg gccgggtccg ctggctggct cccatgccgg
    15421 gggagcacaa tgaagaggtc ttcatgggcg ttctcggcca ctcgcccgag gagctcgagc
    15481 gctggcgggc cgagggcgtg atctgacgag ggcggccgcg cacccggagc cccggcgctt
    15541 gacggcgctc tcgccgccgc gtcagtatcg gtcggcgtga ccagcccctt cgtccccggc
    15601 ccagcggtac ggatcgccgg cgcccccacc gggcccctcg ccggcctgac cttcgccgcc
    15661 aaggatctct tcgacgtcgc gggccacccg accggcgggg gcaacccgga ctgggcgcgt
    15721 cagtacccgg tcccgacgcg ccacgcctgg gcggtgcagc gcctgctcga cgccggcgcg
    15781 acgctgatcg gcaagaccat caccgacgag gtctcgctcg gcatcctcgg ggagaacccc
    15841 ttcgacggca cgccgctcaa tccgaaagcg cccgatcgcg tgcccggcgg atcgtcgtcg
    15901 gggtcggcct cggcggtggc ccagggcctc tgcgacaccg cgctcggcac cgacaccggc
    15961 ggatccgtgc gggtgcccgc gagcttctgc ggcctccacg gcatccggcc gacccacggc
    16021 cggctcgacc tcaccggcat gctgccgcag gcgcccagct ccgacaccgc cggctggttc
    16081 gcccgcgacg cgcagacctt cgcacgcgtc tccgaggtac tgctcggcga gccgatcccg
    16141 cccgcgcggc ccgcgcggct cgtcgtcgcg caggacgcct tcggcctggc cgacgaggag
    16201 acggccgccg cgctccggcc gatggtgcgc cgcctgggcg agctggtgcc cgacgtgcgc
    16261 gaggagatca tggcgccccc cgggctcacc gcctgggcgc gggcccagcg cacgctgcag
    16321 ccgtgggagg cgtggctcac gttcaaggag tggatcgatc gcgacaaccc gcgcatgcag
    16381 tggagcgtcg cccgcaatct cgccctcggg agcgcgatcc ccgagagcga gcgccagtgg
    16441 gccgcgctgg tgcgcgccga ggcccgcgcc cggctcgccg cgctgctgcc gccgggcgcg
    16501 atcctctgcc tgccgaccac gccgttcccg gcgccgctga agggcctgcc gctcgcgaca
    16561 ctcgagccgc tgcgcgcgcg catcacgtgc ttggccgcgc acggcggcct caccggcctg
    16621 ccgcaggtga gcctgccggg cgcgctcgtc ggcgggctac cggtgggcct gtccatcctg
    16681 ggcccccgtg gcagtgacgc catgctggtg gcggtggcga cggcgatgga agccgcccga
    16741 tgaccgatct cacacccaac atccccgagg tggtcgccga ggtcggcgcg ctgttcgagc
    16801 ggtacgagca ggcgctgatc gacaagaccg tcgaggtcct cgacgccacc ttctgggaca
    16861 gccctcacac catccgctac gcgctgcacg agaacggcta cggattcgcc gcgatccacg
    16921 cccatcgcgt cgcgcgcccg ccggggcccg gcatcaagga gcggcgcctg cgcctcgaga
    16981 tcctcacgct cgggcgcgac ctcgccaccg tgaacctcga gttcaaggtg cggggccgcg
    17041 acctgatcgg ccgccagagc cagacgtggg tgcgcttccc ggatctcggc tggaaggtga
    17101 tcggcgccca cgtctccacc atgaacgcgg ccccgatctg gtgagcaccg aaagatgacg
    17161 aagcactcgg cccggcgctt ggtatcatcg cgggcgttca ctcgatcacc ctcgaggagg
    17221 gatctcatgc ggaaagcgat tggcctcgcc gtcctgttgc tcgtcctgtc ggctgccttt
    17281 gggatcggtc tggccgcgta tcccgaccgt ccggtgaagc tcatcgtgcc gtgggccgcc
    17341 ggcggggaca ccgacaacat cttccgaccg ttcgtccccg cgctgcagaa agcgctgggc
    17401 ggcaccgtgg tcatcgccaa cgtggcgggc gcctccggga ccgtgggcgc ccgcgaggcc
    17461 aagaacgctc cccccgacgg atacacgctc tacgccgtcc acgactacat ccactccacg
    17521 tactacacgg gtgtctccga cgtgagctac accgacttcg agccggtctg cctgctctcg
    17581 tcgacgccgt ccgtgctcac ggcgagcccg aagaccaagt ggtcgtcctg gcaggagctc
    17641 ctcggcgacg ccaaggcgcg gccgggccag atcagcgtgg gcgccacgct cggctccacc
    17701 agccacttct tccccgcgct gatcgagaag gcggcgggca tcaagttcaa gtacgtctcc
    17761 tacgaggggc tggccccgcg gatgaacgcg atcctgggcg gccacatcga tctcaccgac
    17821 tcgaacctca cccagaaggg caaggtcgac gccggccagc tccggttcct cgccatcgcg
    17881 accgagaagc ggagcccgga gatccccagc gtgcccacgc tcaaggaact gggggtcaac
    17941 gtggtgttct cggtcgagcg cgggatcatg gcacccaagg ggacggcggc cgacgtcctg
    18001 gccaagctcg agggagcctg cgtccaggcc accaaggatt ccgccttcgc cgagtccatg
    18061 aagaaacagg ggaccgaggt caagtatctc gaccgcaagg cgtacgcgga atacctggcg
    18121 aagctcgaca cgctcaacaa ggacctggcg cgcgacctgg gcctgctcaa gcggtagccg
    18181 cccggtgctc ggccgtgacg ggatcgcggg gctggtgtgt ctcgcgggga gcctcgggct
    18241 cctcgcgctg acgcgcgggc tgccccggtc cgccctcgtg ccgatcggcg cgggcttcta
    18301 cccgcgcatt gtcctggtgg tcaccgcgct cttctccgcg ctgctcatcg tgagcgacgt
    18361 gcgcgcccgc cgccaccacc cggtggccgc gcccgaagcc gggcggcggg cgcccagggc
    18421 cgcgtcccgc aactatcgcc tggtcgcgct caccttcatc gtcttcacgg cctacgtctt
    18481 cgcgctgccg gtcctcggct atcgcgccgc gaccgtctgc ttcgtcctag cgctccagct
    18541 ccttctccaa cccgccggcc ggcggccctg ggtccgcatc gtcgcggtgg ccctcgtgac
    18601 ggcggtgatc acccacctcg tcttcgaaag ctatctctcc gtgctcctcc cgcgcgggcg
    18661 ctggacgggg ttctgagccg tgttcgagct cctcgccgcg gggatcgtca acgtcgccca
    18721 gctcaagtac ctgatcccgc tgctcgtggg gacgctgggc ggcgtggtgg gcggcgcgct
    18781 tcccggcgtg accatcacca tgaccatcat cctggtcctg ccgttcacct acggcctgga
    18841 cccgctccag ggcctcgccg ccatgaccgg cgtctacgtc ggcggctcgg cgggcgggct
    18901 catcaccgcc tgcctgatcg gcatccccgg cacgccctct gccatcgcca ccacgttcga
    18961 cggctttccc atggcgcgca agggcgagcc cggccgcgcg gtgtggctcg gcacgtgggc
    19021 ctcgttctgg ggcggcatcc tcggcggcct cttcctgatt ggcgccaccg cgccgctcgc
    19081 cgccttcgcg ctcgagttcg ggccgtggga gtacttctcg ctcttcatct tcgcgctctc
    19141 catggtggcg gggctgaccg aagcatcgct cctgaagggc ctgctctccg gcgccatcgg
    19201 cctcgtcgtc acggtgatcg gctccgaccc gatcatggcc gtgccgcggc tcaccatggg
    19261 ctcggagttc ctgcgggggg gctttcagtt cctgccgatc ctcatcggcg tcttcgcctt
    19321 cgcccagatc atggccgacg tcgagaagat ggggcggcgg ggcgaaacgg tccaggcggt
    19381 gttcgccccg agtctcgcgg tgtcgcaccc caagatcatc tgggagatcc tgagccggcc
    19441 gttcctgctc ctctggtcga cggtgatcgg cattctgatc ggggtgctgc cggccatcgg
    19501 cggcagcgcc gccaacatca tggcctacga ccaggccaag aagttctcgc gccatccgga
    19561 gcgcttcggc accggcacgc ccgagggcat catcgcctcc gagtcgtcca acaacgccaa
    19621 cgtcggcggc tcgctcgtga ccatcatggc cttcgggatc ccgggggacg cggtgaccgc
    19681 cgtcatgctg ggcgccatga tcatccacgg catccagccg gggccgctct tcatcagcca
    19741 gaatccggac ctcgcctacg gcatcttcgc cgcctacatc cttgcccacc ccatcatggt
    19801 gctgatcatc gccgccggcg cccgcttctt cctgcacatc gtctccgtac ccaaggcggt
    19861 gctgattccg gtggtgctcg tcctctgcgt gatcggcgcc tacgccctca acaacatcat
    19921 ggagaacgtc tacgtcctcc tgctcttcgg cctcctgggc tacgccttcg tcaagctcgg
    19981 cttcccgctc gccccgctga tcctcggcgt gatcctgggc gatcagatcg aggtcaactt
    20041 ggtccgcgcc atcatgacgg atcccgaccc ctggctcttc ctgacccgcc cgatctcggg
    20101 tgcgctgatc gcgctctcgg cggcgtcggt gctcgtcgcg ctctggcagc accggcgcgc
    20161 ccacacccgc atgggaccgc cgcccgagca ggcggtggac ttctaaagcg gccggccgcg
    20221 catgatccgg gggagcgccg ccgcgacggg ggacatcgtc catcgggcgg gcggcgcgac
    20281 ctccatcttc gagggaaacc ggatcgagcg ctgcttccgc gacgtgcaca cggccacccc
    20341 gcatggagcg ttggccccga ggacgctgga gctggtcggg caggcgcgcc tgggcgtgga
    20401 ctcccagggt ctgctctgat cgtcccgctc ccacgttgac accatggccg cccgccctta
    20461 tggtgcgggc tcaaggaggc cacgcccatg ctgcagcgcc aggtcggtga ggtcatcatc
    20521 aaccgcgtca tcgagtccga gcggccggac ttcgacgccg ccgggttctt ccccaagatt
    20581 acgcccgaac agtgggcgcc ctaccggcag cggctcgcgg gctgggccat ggacaccggc
    20641 gtgaacggcc tcgtcttccc gatgcagtcg ttcctgctcc ggacgcggca ccacacgatc
    20701 gtggtggaca cctgcgtggg cgaccacaag gagcgggcgc ggcccacctg gaacatgacg
    20761 tcgagcggcg agtatctgaa gcggttcgcc gagaccggcg tccggccgga gcaggtggac
    20821 ttcgtgatga ccacgcactt ccacaccgac cacgtcggtt ggaacacgcg ctgggacaac
    20881 ggccgctggg tccccacctt ccccaacgcc aagtacgtca tgtccgagaa ggagtggacg
    20941 tactggtcgg ccatgcacaa ggagaccccg cagaaccaga tcgcggacag cgtgatcccc
    21001 atcgtcgagg cgggccgcgc ccagatggtg cgaaacgact tcgcgctcga cgacgaggtc
    21061 cgcttcgagt cgaccgcggg gcacacgccc gaccacatga gcgtgcacgt ccagtccaac
    21121 ggccagcacg cggtgatcac cggcgacctg atccactccc cggtgcagct ccaggagacc
    21181 gcgtgggtgc cgcggcccga cttcgacccc gcgc
//
LOCUS       DASUFA010000204         8561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_50709, whole genome shotgun sequence.
ACCESSION   DASUFA010000204 DASUFA010000000
VERSION     DASUFA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50709"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFX87_07425"
     CDS             <1..207
                     /locus_tag="VFX87_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4994781.1"
                     /translation="LPLPEFHRQVASLFGWVKSAPLAQGATEILIPGEPEARLEAERR
                     RQGIPIEDQTWSQIETTAAELGVA"
     gene            complement(219..1061)
                     /locus_tag="VFX87_07430"
     CDS             complement(219..1061)
                     /locus_tag="VFX87_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994782.1"
                     /translation="MRLRITARLLSLLALALVALGAEATRAQSPDLPIFDTHIHYSAP
                     DWTEYPPDRILGILEKAGIKRALVSSTPDDGTLTLHAKDPKRIVPILRPYRTRGDMGH
                     WWQDPAVLAYVQERLQKNRGVHKGIGEFHLFGGQTGTFVVKRITELAVQENIYLHAHS
                     DELAIIELFTIEPRLRVIWAHAGMSSGPQAVGALLDRYPNLWVDLAIRNGDVAPGGTL
                     DPGWRAVFLRHPDRFLAGTDTWMTSRWDALPGSVTEVRSYLSQLPRDVAEKIAFRNAE
                     RLFP"
     gene            1127..1480
                     /locus_tag="VFX87_07435"
     CDS             1127..1480
                     /locus_tag="VFX87_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994783.1"
                     /translation="MRRAIVIVSLLLAAGPAAAQSLPGGRPPDAIDRARGLATQPFST
                     QPAPERPAERYVPPARVYSPAQGREVLVPGHYERDVNGQRVEVPPLVTTTPDGRNPVV
                     VPGGERPAIEPRGGP"
     gene            1480..1698
                     /locus_tag="VFX87_07440"
     CDS             1480..1698
                     /locus_tag="VFX87_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994784.1"
                     /translation="MLEAVGMAEINKIFAVTDALGIHREALVIPLGTGKGRVRKLPSG
                     KFEITVDAETPLDEWLEGLPALIRAAMA"
     gene            1695..2999
                     /locus_tag="VFX87_07445"
     CDS             1695..2999
                     /locus_tag="VFX87_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX4994785.1"
                     /translation="MSPRRRGEIGLLSLVAILFFNVSGGPYGLEDAVGTLGPGLALVL
                     LVVTPLVWSLPVSLAMAELAAALPEEGGYVVWVQRAFGRFWGFQVGWWSWINSFVDVA
                     VYPALFADYVGFWRPEMSSLERWALVLAFIWILTAINLAGVRITGWVAVGMAGVSLLP
                     VVVLTAVAGTQLREVPWRPFAAEEGSLLTGLGLGLAIMMWNYSGWDAPTTTLGETQDP
                     GPSFRRAVWVALPLITLAYVLPVAASLSAVEGWEDWDTGHWPVVAEAIGGPWLANLVA
                     TGAVIATAGLFLSLLLTNSRLPYVLALAGQMPAALGRLHPRFGTPWSAVVLSSACYSV
                     CAFWSFKELIVLNIWLYSITLVLELAAFVALRLREPGLPRPWRVGGGKAGMWLVAGLP
                     SLCCLLAMATAGWLNIAVGVGAALTGPLAYLLWRAPASRAMR"
     gene            complement(2980..3828)
                     /locus_tag="VFX87_07450"
     CDS             complement(2980..3828)
                     /locus_tag="VFX87_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX4994786.1"
                     /translation="MNRALAGAPSGGRPLVVVDLGGTWVRVSVSGAAPRAYRGPTPGV
                     AGLPALLARLWRRWRLSRGDLAGLGVASRGIWTRGERGDLARRLRRFAPRVVVISDVE
                     AAYLAALGAVPGVLLLAGTGSIALGRDRRGRFAREGGLGPLLGDDGSAFSIGRAWLRG
                     AVAPARARRIATAPDAVARVAALAPGVLRRARRGDRTARRVVREAQQALAALAGRTAR
                     ALRLRQPVTLSWAGGLLGDAPFRAGVWRHLRGQGLRIRPVRPKAPAASPRAWRTVFPA
                     VTSSHD"
     gene            3953..4558
                     /locus_tag="VFX87_07455"
     CDS             3953..4558
                     /locus_tag="VFX87_07455"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994787.1"
                     /translation="MRRTLMTMTAALLLGLTASVAAALTPTETVKSRVDQALQSLSQT
                     ANPGAEASERRRADIRRAADTLFDFPDMSRRALGRHWTDRTPAEREEFTRLFTDLIAR
                     TYIGKIDRYAGESIAYVGERVDGEEASVRSQVVTAKGSQIPVEYRLHRVKDEWTAYDV
                     LIENVSLVGTYRSQFDRIIKAESFANLLRRLREKGQVAEAQ"
     gene            complement(4583..6412)
                     /locus_tag="VFX87_07460"
     CDS             complement(4583..6412)
                     /locus_tag="VFX87_07460"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4994788.1"
                     /translation="MLRAMLVGLLVSVAVTAFSRTGVLAGWETRAVDAFLFLRDRVAA
                     PEITLVLIDDDAFQALGQRQPLSRRYVADLADFLLKSGARVVAFDLVLTAPTVPAEDQ
                     ALLATIGRWSAVRPGALLFATFAMPATQDGADGYTQLRPFAPELAGLVGFANAPIGAD
                     GMVRRFTPVLPRAGGGWLPALSLVTLASSSGLPPAELAKRLAGDAPIPLPLREASGAL
                     GGPMETPVSQLAARPWRIDFTGPAGAFTAFPSEPLVQLARAGTVPDQDNPFRDRIVLV
                     GASFAESRDTFPTPTGPMPGVEIHAHMVNTLLSRRTLLPPPWYLNVALLTLVCVSISV
                     LSLWLRPAWLALVGLVLVGGLAAASYEAYTRGGYWLDFLAPLIGMLGYLQGAHMLRRR
                     RLRSAFGQYVSPEIMDRVLRTGAPLGGEVRRVTVLMSDLRGFTTMSEGLAPEVVSEMM
                     NEYFTAMVDVILAHRGLVQDFIGDAIMAVYGAPLDDPEHCWHAAHTAVAMHAALEDLN
                     RRWEAVGRGPLAMGIAVHTGEAFAGTLGAPQKKKYAVLGDTVNTTSRIEGLNRDLGTG
                     ILISGAVLAVLKDRVVVRDRGRVTVKGRRQPVEIYELTSLTEA"
     gene            6609..8357
                     /locus_tag="VFX87_07465"
     CDS             6609..8357
                     /locus_tag="VFX87_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HEX4994789.1"
                     /translation="MLSPAAELLIDGAIVVTYFTGITAIGLYLGRRDRSLEDFALAGR
                     RVPWWAVMASIIAAETSAATFLGVPVEGYTTRSFAYVQIMVGLILGRVVVGNVFLRPY
                     YTFRVYTVYDYLGVRFGPWTKAYVSALFLFMRTLASGTRLFIPSLVMVLAYRLFVSGA
                     RAEAAQEPVTTVGPYLIAIVILTVVTCLYTAVGGIKAVIWTDVIQACLMFGGALVAIA
                     TLLVHVGGLSEVVRAVPQLASHEGYFLHGFEADRVAAWQTARGLSSMGVWDYVKLVLA
                     SDYTLFSALIGATVGNMAAFGTDQDMVQRMLTAETHQKARRSLVTAAFMDVPIAGAFV
                     FIGILLFVFYQQDPTFRPGANADVFASYILNVMPVGVRGFVVAGVFATAMGSFSAALN
                     ALATSATNDWYLHWVRGRSQIHYVRVARGFTVLFAALMVAIAGAFAYAKVTTPDVRII
                     PVVLGIAGFILGPMLGVFLIGMLTTGRGSDRGNLLAITAGLLATVVAGKLHIMILNGV
                     APWLGLAATFRQPAWIPEVAFTWWAMVGAVVVVAVGLCFRTPAPVRAAVARHEREAQM
                     GEEIPVELRGTRNPRG"
BASE COUNT         1158 a         3050 c         3146 g         1207 t
ORIGIN      
        1 ctgccgctgc ccgagttcca ccgccaggtg gcctcgctct tcgggtgggt gaagtcggcg
       61 ccgctcgcgc agggcgccac cgagatcctg atcccggggg agccggaggc gcggctcgag
      121 gcggagcgcc gccggcaggg gattcccatc gaggatcaga cctggagcca gatcgagacg
      181 acggcggcag agctgggtgt cgcctagcga cggcggggct acgggaagag ccgctcggcg
      241 ttgcgaaagg cgatcttctc cgccacgtcg cgcgggagct ggctcaggta gctccgcacc
      301 tcggtcaccg agcccggcag cgcgtcccag cgcgacgtca tccaggtatc ggtcccggcg
      361 aggaagcggt cgggatggcg gaggaacacc gcgcgccagc ccgggtcgag cgtgcccccc
      421 ggggccacgt caccgttgcg gatggccaga tccacccaga ggttcgggta gcggtcgagc
      481 agggcgccca ccgcctgcgg gcccgaggac atgcccgcgt gcgcccagat gacccggagg
      541 cgcggctcga tcgtgaacag ctcgatgatc gcgagctcgt ccgagtgggc gtggagatag
      601 atgttctcct gcaccgccag ctcggtgatg cgcttgacca cgaaggtgcc ggtctgcccg
      661 ccgaagaggt ggaactcgcc gatgcccttg tgcacgccgc ggttcttctg caggcgctcc
      721 tgcacgtagg cgagcaccgc cgggtcctgc caccagtggc ccatgtcgcc gcgggtgcgg
      781 tagggccgca ggatcggcac gatgcgcttg ggatccttgg cgtggagcgt gagggtgccg
      841 tcgtcgggcg tgctggatac gagcgcgcgc ttgatccccg ccttctcgag gatgcccagg
      901 atgcggtcgg gtgggtactc ggtccagtcg ggggcgctgt agtggatatg cgtgtcgaag
      961 atcggcaggt ccgggctctg ggcgcgcgtg gcctccgctc cgagggcgac gagcgccagg
     1021 gcgagcaggg aaagcaggcg cgccgtgatc cgcaaccgca cggctggagc ctagcatgga
     1081 cgccgcgcct tgcgcacccg catcggcggg gctacgcttg atgcggatgc gtcgcgcgat
     1141 cgtgatcgtg agcctgctgc tggcggcggg gcccgccgcc gctcagagtc tgcccggcgg
     1201 ccgtccgccc gacgccatcg atcgggcgcg cgggctcgcc acgcagccct tcagcacgca
     1261 gcccgcgccc gagcggcccg ccgagcgcta cgtgccgccg gcccgcgtgt actcgcccgc
     1321 ccaggggcgc gaggtgctcg tcccgggcca ctacgagcgc gacgtcaacg gccagcgcgt
     1381 cgaggtcccg ccgctcgtca ccaccacgcc cgacgggcgg aacccagtgg tggtccccgg
     1441 cggggagcgt cccgcgatcg agccgcgcgg cgggccctga tgctcgaggc ggtgggcatg
     1501 gccgagatca acaagatctt cgcggtcacc gacgccctcg ggatccaccg cgaagcgctc
     1561 gtgatccccc tcggcaccgg caagggccgc gtgcgcaagc tgccgagcgg caagttcgag
     1621 atcacggtgg acgcggagac gccgctggac gagtggctcg agggattgcc cgcgctgatc
     1681 cgagccgcca tggcgtgagc ccccgccgac gaggggagat cgggctcctc tccctcgtcg
     1741 ccatcctctt cttcaacgtc agcggcgggc cctacgggct cgaggacgcc gtcggcaccc
     1801 tcggccccgg gctcgcgctg gtcctcctcg tggtcacccc gctggtctgg agcctccccg
     1861 tctcgcttgc gatggcggag ctcgccgccg cgctgccgga agaaggcggc tacgtggtgt
     1921 gggtgcagcg tgccttcggc cgcttctggg gcttccaggt ggggtggtgg agctggatca
     1981 acagcttcgt ggacgtggcg gtctatcccg cgctcttcgc ggactacgtc gggttctggc
     2041 ggccggagat gtcctcgctc gagcgctggg cgctggtgct ggccttcatc tggatcctca
     2101 ccgccatcaa cctggccggg gtgcgcatca cgggctgggt cgcggtgggg atggccggtg
     2161 tgtcgctcct gccggtggtc gtgctcaccg cggtggcggg cactcagctc cgcgaggtgc
     2221 cgtggcgccc cttcgccgcg gaggagggga gcctcctcac cgggctcggc ctggggctcg
     2281 cgatcatgat gtggaactac tcgggctggg acgcgcccac caccacgctc ggcgagacgc
     2341 aggacccggg cccgagcttc cgccgcgcgg tgtgggtcgc gctgcccctg atcacgctcg
     2401 cctacgtgct gccggtggca gccagcctct cggcggtgga gggctgggag gactgggata
     2461 ccggccactg gccggtggtc gcggaggcga tcggcggacc gtggctcgcc aacctggtcg
     2521 ccaccggcgc ggtgatcgcc accgcggggc tcttcctgtc gctgctcctg accaactcgc
     2581 ggctgcccta cgtgctggcc ctggccggcc agatgccggc ggcgctcggg cggctccacc
     2641 cgcgcttcgg cacgccgtgg agcgcggtgg tgctgtcgag tgcctgctac agcgtgtgcg
     2701 cgttctggag cttcaaggag ctgatcgtgc tcaacatctg gctctattcg atcacgctcg
     2761 tcctggagct ggccgccttc gtggccctgc ggctgcgcga gccggggctg ccgcggccgt
     2821 ggcgcgtggg cggcggcaag gccgggatgt ggctggtggc ggggctgcca tcgctctgct
     2881 gcctgctcgc gatggcgacc gcggggtggc tcaacatcgc ggtcggcgtg ggcgcggcgc
     2941 tgaccggacc gctcgcctac ctgctctggc gggcgccggc tagtcgtgcg atgaggtaac
     3001 cgctgggaac accgtgcgcc aggcgcgcgg gctcgcggcg ggcgccttcg ggcgcacggg
     3061 ccggatgcgg aggccctggc cgcgcaggtg gcgccagacc ccggcgcgaa acggcgcgtc
     3121 ccccagcagg ccgccggccc agctcagcgt gaccggctgg cgcaggcgca gggcgcgcgc
     3181 ggtgcgtccg gcgagggctg caagcgcctg ctgcgcctcc cggacgacgc gccgggcggt
     3241 ccggtctccg cgccgggctc ggcggaggac gccgggcgcg agggcggcca cgcgcgcgac
     3301 cgcgtcgggc gccgtcgcga tccggcgtgc gcgcgccggg gcgaccgcgc cgcgtagcca
     3361 ggcgcggccg atcgagaacg cggagccgtc gtcgcctagg agcgggccga ggccgccttc
     3421 tcgcgcgaag cgcccgcgcc ggtcgcggcc gagggcgatg gagccggtgc cggcgagcag
     3481 cagcacgccg gggaccgcgc cgagcgcggc caggtaggcc gcctcgacgt ccgagatcac
     3541 gaccacgcgc ggggcgaagc ggcgcaggcg ccgcgcgagg tccccgcgct cgccgcgggt
     3601 ccagatccca cgcgaggcca cgccgagccc ggccaggtcg ccgcgcgaga gccgccaccg
     3661 gcgccagagg cgcgcgagca gcgcgggcag gcccgccacg cccggcgtcg gcccccggta
     3721 ggcgcgcggc gcggcgcccg acacggacac gcgcacccag gtgccgccca ggtccaccac
     3781 gacgagcggc cggccgccgg acggggcccc cgcaagagcc cgattcatgg cgctattgtc
     3841 ccccggtgcc ccgggcggcc gcgcataatg aaagcacgcc aaggtcggaa tacccacgtg
     3901 cgcggcacgt cgaatcagac agtgcagtga ctctctcgaa cctggaggca atatgcgacg
     3961 taccctgatg accatgacgg ccgcgctgct cctgggcctg accgcgtccg tcgcggctgc
     4021 cctgacgccg accgagaccg tgaagtcgcg agtggaccaa gcgctgcaga gtctctccca
     4081 gaccgccaac cccggcgccg aggcctccga gcggcggcgg gcggacatcc gccgggcggc
     4141 cgacacgctg ttcgatttcc ccgacatgtc gcgccgggcg ctgggccgtc actggacgga
     4201 ccgcacgccc gccgagcgcg aggagttcac gcggctcttc accgacctga tcgcgcgcac
     4261 gtacatcggc aagatcgacc gctacgcggg ggagtccatc gcctatgtgg gcgagcgcgt
     4321 ggacggcgag gaggcctcgg tgcggtcgca ggtggtcacc gccaagggct cgcagattcc
     4381 cgtcgagtac cgcctgcacc gcgtgaagga cgagtggacc gcctacgacg tgctcatcga
     4441 gaacgtgagc ctggtgggta cctaccggag ccagttcgac cgcatcatca aggcggagtc
     4501 gttcgcgaac ctgctgcgcc gcctgcgcga aaagggccag gtcgcggaag cgcagtagcg
     4561 cgagtggccc gcccgcatca cctcaggcct ccgtcaggct ggtgagctcg tagatctcga
     4621 ccggctgccg ccgccccttc acggtcacgc ggccacggtc ccggaccacc acccggtctt
     4681 tcaggacggc cagcacggcg ccgctgatca ggatgccggt gccgaggtcg cgattcaacc
     4741 cctcgatgcg cgaggtggtg ttgacggtgt cgcctagcac cgcgtatttc ttcttctgcg
     4801 gagcccccag cgtccccgcg aaggcctccc cggtgtgcac cgcgatgccc atggcaagcg
     4861 gcccacggcc cacggcctcc cagcgccggt tcaagtcctc gagcgccgcg tgcatggcca
     4921 ccgcggtgtg ggccgcgtgc cagcagtgct cggggtcgtc gaggggcgcg ccgtagaccg
     4981 ccatgatcgc gtcgccgatg aagtcctgca cgaggccgcg atgggcgagg atcacgtcca
     5041 ccatcgcggt gaagtactcg ttcatcatct cggagacgac ctcgggggcc aggccctcgg
     5101 acatggtggt gaagccgcgc agatccgaca tcagcacggt cacgcggcgc acctcgccgc
     5161 ccagcggcgc gccggtccgc agcacccggt ccatgatctc ggggctgacg tactggccga
     5221 aggcggagcg caggcggcgg cggcgcagca tgtgggcgcc ctgcaggtac cccagcatgc
     5281 cgatcagcgg ggcgaggaag tcgagccagt agccgccgcg cgtgtaggct tcatacgaag
     5341 cggcggcgag cccgcccacc aggaccaggc cgaccagcgc gagccaggcc ggccgcagcc
     5401 agagcgagag caccgagatg gacacgcaga ccagcgtgag cagcgccacg ttcaggtacc
     5461 agggcggcgg cagcagggtc cgacgggaga gcagggtgtt caccatgtgg gcgtggatct
     5521 ccacgccggg catgggcccc gtcggagtcg ggaacgtgtc acggctctcc gcgaagctcg
     5581 cgcccaccag gacgatgcgg tcgcggaagg gattgtcctg atcggggacg gtaccggcgc
     5641 gtgcgagctg caccagcggc tcgctcggga acgcggtgaa ggcgcccgcg ggcccggtga
     5701 agtcgatgcg ccacggccgg gccgcgagct ggctcaccgg cgtctccatc ggcccgccga
     5761 gcgcgccgga cgcctcgcgc aggggcaggg ggatcggggc gtctcccgcg agccgcttcg
     5821 ccagctccgc gggcggaagg ccgctggacg aggcgagcgt gacgagcgac agcgcgggca
     5881 gccatcctcc ccccgcccgg ggcaggaccg gcgtgaagcg ccgcaccatg ccgtcggcgc
     5941 cgatgggcgc gttggcgaag cccaccagcc cggccagctc cggcgcgaag ggccggagct
     6001 gggtgtagcc gtccgcgccg tcctgggtgg cgggcatcgc gaaggtggcg aagagcagcg
     6061 caccgggccg caccgcgctc cagcgcccga tggtcgcgag cagcgcctgg tcctccgcgg
     6121 gcacggtggg ggcggtgagg acgaggtcga acgccaccac ccgcgcgccg ctcttgagca
     6181 ggaagtcggc gagatcggcg acgtagcgcc gcgagagcgg ctggcgctgc ccgagggcct
     6241 ggaacgcgtc gtcgtcgatc aggaccaggg tgatctcggg cgcggccacc cggtcacgca
     6301 ggaagaggaa ggcgtccacc gcgcgcgtct cccagcccgc gagcacgccg gtgcgggaga
     6361 acgcggtgac cgcgacggag accagcaggc cgaccagcat cgcgcgcagc acccgcgcgc
     6421 ggcgtcccgt gccccggagg gccagggtca gtggccgact ccggtgatcc agcgggtgcc
     6481 gggggatggg aattccatgg ggatcatcga gtcataccgg agcgatgata gaccagcgcc
     6541 gcgaccggca gcaaggcgcg agcgccgtag attgccgcct ccgggcccgg cctccgcgat
     6601 aatggatcgt gctctctccg gcggcggagc tgctgatcga cggcgccatc gtggtcacct
     6661 acttcaccgg catcaccgcc atcggcctct acctgggccg gcgggaccgg agcctggagg
     6721 acttcgccct cgcgggccgc cgcgtgccct ggtgggcggt gatggcctcg atcatcgcgg
     6781 cggagacgag cgcggcgacc ttcctgggtg tgccggtcga gggctacacc acccggagct
     6841 tcgcctacgt gcagatcatg gtcgggctga tcctggggcg cgtcgtggtc ggcaacgtct
     6901 tcctgcggcc gtactacacc ttcagggtct acaccgtcta cgactacctc ggggtgcgct
     6961 tcggaccgtg gaccaaggcc tacgtctccg ccctgttcct cttcatgcgc acgctcgcct
     7021 ccggcacgcg gctcttcatc ccctcgctcg tgatggtgct ggcgtaccgc ctgttcgtct
     7081 cgggggcccg cgcggaggcc gcgcaggagc cggtcaccac cgtcggcccc tacctgatcg
     7141 cgatcgtcat cctcaccgtc gtgacctgtc tctacaccgc ggtgggcggg atcaaggcgg
     7201 tgatctggac cgacgtgatc caggcctgct tgatgttcgg cggggcgctg gtggcgatcg
     7261 ccaccctgct cgttcacgtg ggcggtctct ccgaggtcgt ccgggcggtg ccgcagctcg
     7321 cgagccacga gggctacttc ctgcacgggt tcgaggccga ccgcgtcgcg gcgtggcaga
     7381 ccgcgcgcgg cctctcctcg atgggggtgt gggactacgt caagctggtc ctggccagcg
     7441 actacacgct cttctccgcg ctcatcggcg ccaccgtcgg caacatggcc gccttcggca
     7501 ccgaccagga catggtccag cggatgctga ccgcggagac ccaccagaag gcgcgccgca
     7561 gcctggtcac cgccgccttc atggacgtgc cgatcgccgg cgccttcgtc ttcatcggca
     7621 tcctgctctt cgtgttctac cagcaggacc cgacgttccg gccgggggcc aacgccgacg
     7681 tcttcgcctc ctacatcctg aacgtcatgc cggtcggcgt gcgcggcttc gtggtcgcgg
     7741 gagtgttcgc caccgcgatg ggctccttct cggccgcgct caacgcgctc gccaccagcg
     7801 ccaccaacga ctggtacctc cactgggtgc gcggccggtc ccagatccac tacgtgcggg
     7861 tggcccgcgg gttcaccgtc ctcttcgcgg ccctcatggt cgcgatcgcc ggcgccttcg
     7921 cctacgccaa ggtcacgacc ccggacgtgc ggatcatccc ggtggtgctc ggcatcgccg
     7981 gcttcatcct gggcccgatg ctcggcgtgt tcctgatcgg gatgctgacc accggccgcg
     8041 gctccgaccg gggcaacctg ctggcgatca ccgcggggct gctcgcgacg gtggtggccg
     8101 gcaagctcca catcatgatc ctgaacgggg tggcgccctg gctcggcctg gccgcgacct
     8161 tccggcagcc ggcctggatc ccggaggtgg cgttcacctg gtgggccatg gtcggcgcgg
     8221 tggtggtcgt cgccgtcggc ctctgcttcc gcacgcccgc cccggtgcgc gcggcggtgg
     8281 cccgccacga gcgcgaggcg cagatggggg aggagatccc cgtggagctg cggggcaccc
     8341 ggaaccctcg cggctgaccg gcccgcccgg ccggggtttc ctgaacggcg gttcagtgct
     8401 atccttccgc cactgaacgg ccaatcactc ctcgaagcgg aggatgccat gtcgagcacg
     8461 ttcctctacc ggctgcccgt cgaacagacc cagtggaagg tcgagggcca gaccaccacc
     8521 gcgttcacct gggaatacga ggacggcagc gacgacctgc t
//
LOCUS       DASUFA010000205         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1288627, whole genome shotgun sequence.
ACCESSION   DASUFA010000205 DASUFA010000000
VERSION     DASUFA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288627"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VFX87_07470"
     CDS             <1..1390
                     /locus_tag="VFX87_07470"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEX4994790.1"
                     /translation="AAVELEVDGARQAVFDQAHRVGARARTYVPGGVPIELPYHAHEQ
                     RLEATRRALAEGAPAVYEAAFRADGVFVSVDILERRGPGFCLTEVKSTTSVKEHHLPD
                     VAVQAHVLRRSGLDVARMEVMHLNRACTYPDLSDLFVRADVTAPVEMRLAAVPGEIAA
                     MTAMLAGPLPSVATGPHCSAPYECPFTERCWPVRPPHHVSTLHAMRRRALELDELGYR
                     TIFDLPEDFPLKAAADRQRRAVQNGQMVVEPTLAAALARFAPPVAFLDFETVGLAIPV
                     WDGCHPYDAVPVQFSAYVPDGDGALRPHAWLAAGPGDPRPALAERLLAACEGARAIVA
                     YNARFEADRIRELAVALPHLAARLEALAASLVDLLPVVRNHVYHPDFGGSFSLKRVLP
                     ALVPEMSYRGLAIAEGETASLELGRLLFYGDAMDPEERARLRADLLAYCHQDTWGLVR
                     LLERLRGLAGGA"
     gene            1387..2385
                     /locus_tag="VFX87_07475"
     CDS             1387..2385
                     /locus_tag="VFX87_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994791.1"
                     /translation="MTGPIDVHTHIFASGWPDFAARYGGDRWPRLEGDPATGCKLYLG
                     AAFNRNLTPHAFDPARRLEQMDKSGVDRQLLSPAPPTFCYWAPGPAAAEWCRMQNDAI
                     AELVSRSPARFLGAGGLPLQAPDLAVKELERVATVLRFPAVEIGANVGGNDLDDPSLA
                     PVWDAAAALGVAIFVHPQAPILGDPRFRKNNLTQVAGFPLETALAMTRIIFGGVLDRW
                     PSVRWCFAHGGGAFPYILPRLDQGWSAIPDAKQALAQPPSAYARRVWVDSLTLSPKVL
                     AFALDTFGPERIVLGSDYPFKMGVDDPVGALDALALDGATRRRLTVDNARQFLALE"
     gene            2393..3415
                     /locus_tag="VFX87_07480"
     CDS             2393..3415
                     /locus_tag="VFX87_07480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994792.1"
                     /translation="MNFGTFLLMQSPSARSSKEIYARAVEMAQAAESLNFNNIWLAEH
                     HFSTYGYLSRPVQLATYLAGRTTRIRVGTAVIVVPLHHPLVVAEEIATLSLLAPGRLD
                     IGLGRGYQHYEFERFGLELESGRARWEESVDIILKALAGGPFSYEGKLYRIPETTIFP
                     QMEGPRPPIWITAQSPDSVEGAVRRGFNVLTGGFGVPVERMAEFRRLFDRVVSEVKPP
                     QPLHVGVQRAVYVTKNDADARAAAEEARWNMRVTLSLRNHYERVEDGRAIPVPAQKEP
                     DVDDLLDRYLVIGTPETCIHQIERLRRDVGITHFNCSFWFGDLEQGRVLRSMELFARE
                     VMPAFA"
     gene            3564..>3926
                     /locus_tag="VFX87_07485"
     CDS             3564..>3926
                     /locus_tag="VFX87_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX4994793.1"
                     /translation="MADKGPRHAWPGASLARVPYWVYQDDANYRAELRRIFEGPVWNY
                     LCLASDLPKAGDYRTTFLGEMPVIVVRGAEGQIHAFENRCAHRGALIALDDSGSTRGH
                     FQCVYHAWTYDLEGRLVGI"
BASE COUNT          541 a         1454 c         1360 g          571 t
ORIGIN      
        1 ggccgcggtc gagctcgagg tcgacggggc gcgccaggcc gtattcgacc aggcccaccg
       61 ggtcggcgcg cgggcccgga cctacgtgcc cggcggtgtg ccgatcgagc tgccctatca
      121 cgcccacgag caacggctcg aggcgacccg gcgggccctc gcggagggcg cgcccgccgt
      181 ctacgaggcg gccttccgcg cggacggcgt cttcgtctcg gtggacatcc tggagcgacg
      241 gggaccgggc ttctgcctga ccgaggtcaa gtccaccacg agcgtgaagg agcaccacct
      301 gcccgacgtg gcggtgcagg cccacgtgct ccgccgcagc ggcctcgacg tcgcccgcat
      361 ggaggtcatg cacctcaacc gtgcctgcac gtaccccgac ctgagcgatc tcttcgtgcg
      421 ggccgacgtg accgcgccgg tcgagatgcg cctggccgcg gtcccgggcg agatcgcggc
      481 catgaccgcc atgctcgcgg gcccgctgcc gagcgtggcc actggccccc attgctcggc
      541 gccctacgag tgcccgttca ccgagcggtg ctggccggtg cggccgcccc atcacgtcag
      601 cacgctccac gcgatgcggc ggcgcgcgct cgagctggac gagctgggct accgcacgat
      661 cttcgatctg ccggaggatt tccccctcaa ggccgccgcc gatcgccagc gccgggccgt
      721 gcagaacggg cagatggtcg tcgagccgac cctggccgcc gcgctcgccc gattcgcgcc
      781 gccggtcgcg ttcctcgact tcgagacggt cggcctcgcc atcccggtgt gggacggttg
      841 ccacccctac gacgcggtgc ccgtgcagtt cagcgcctac gtgccggacg gcgacggtgc
      901 cctgcggccg cacgcctggc tcgcggccgg gcccggtgac ccgcgcccgg cactggccga
      961 gcggctcctc gccgcatgcg agggcgcccg cgccatcgtg gcgtacaacg cgcgcttcga
     1021 ggccgaccgc atccgggagc tcgcggtcgc gctaccgcac ctggccgccc ggctcgaggc
     1081 cctcgcggcg agcctggtgg acctgttgcc cgtggtgcgc aatcacgttt atcatccgga
     1141 cttcggcggc agcttctcgc tcaagcgcgt gctgccggcg ctggtgcccg agatgagcta
     1201 tcgcgggctc gcgatcgccg agggcgagac ggcgagcctc gagctggggc gcctgctctt
     1261 ctacggtgac gcgatggacc cggaggagcg ggcacggctg cgcgcggatc tgctcgcgta
     1321 ctgccatcag gacacgtggg ggcttgtcag gctgctggag cggctgcgcg gcctggcggg
     1381 gggagcatga cgggaccgat cgacgtccac acgcatatct tcgcgagcgg ctggccggac
     1441 ttcgcggcgc gctacggcgg cgatcgctgg ccgcggctcg agggcgaccc ggccaccggg
     1501 tgcaagctct atctgggcgc cgcgttcaac cgcaatctca cgccgcacgc cttcgacccc
     1561 gcgcggcggc tcgagcagat ggacaagagc ggcgtggatc gccagctcct ctccccggct
     1621 ccgcccacct tctgctactg ggcacccggc cccgccgcgg ccgagtggtg ccggatgcag
     1681 aacgacgcga tcgcggagct ggtcagccgg agccccgcgc gcttcctcgg agcgggcggg
     1741 ctaccgctgc aggcgcccga cctggccgtg aaggagctgg agcgcgtggc gacggtgctg
     1801 cgcttccccg cggtggagat cggcgctaac gtgggcggca acgatctcga cgacccgagc
     1861 ctggccccgg tctgggacgc agcggccgcg ctcggcgtcg cgatcttcgt ccatccgcag
     1921 gcgccgatcc tgggcgaccc gcgcttccgc aagaacaacc tgacgcaggt cgccggcttc
     1981 ccgctcgaga ccgcgctggc catgacgcgg atcatcttcg gcggcgtgct cgaccgctgg
     2041 ccgtcggtgc gctggtgctt cgcgcacggc ggcggcgcct tcccctacat cttgccgcgg
     2101 ctcgaccagg gctggtcggc gatcccggac gccaagcagg cactcgccca gccgccgagc
     2161 gcctacgcgc ggcgggtctg ggtggactcg ctgacgctct cgcccaaggt cctcgcgttc
     2221 gccctcgaca ccttcgggcc cgagcgcatc gtgctgggga gcgactaccc attcaagatg
     2281 ggcgtggacg atccggtcgg cgcgctcgac gcgctcgccc tcgacggggc cacgcggagg
     2341 cggctcaccg tggacaatgc ccggcagttc ctcgcactgg aatgaggaga ccatgaactt
     2401 cgggaccttc ctgctgatgc agtcgccgtc cgcccgctcc tccaaggaga tctacgcccg
     2461 cgcggtcgag atggcccagg ccgcggagag cctgaacttt aataatatct ggctggccga
     2521 gcaccacttc tcgacctacg gctacctctc ccgcccggtg cagctcgcca cctatctggc
     2581 ggggcggacc acccgcatcc gggtgggcac cgcggtcatc gtggtgccgc tccaccaccc
     2641 cctggtggtc gccgaggaga tcgccaccct ctcgctcctg gccccgggcc ggctcgacat
     2701 cgggctcggc cgcggctacc agcactacga gttcgagcgc ttcgggctcg agctcgagag
     2761 cgggcgcgcc cgatgggagg agtcggtgga catcatcctg aaggcgctcg cgggcgggcc
     2821 cttcagctac gagggcaagc tctaccggat ccccgagacc acgatcttcc cgcagatgga
     2881 agggccgcgg ccgcccatct ggatcaccgc ccagagcccc gactccgtcg agggcgcggt
     2941 gcgtcgcggc ttcaacgtgc tcaccggcgg cttcggcgtc ccggtcgagc gcatggccga
     3001 gttccgcagg ctcttcgacc gggtggtgtc cgaggtgaag ccgccgcagc cgctgcacgt
     3061 cggcgtgcag cgcgcggtct acgtgaccaa gaacgacgcg gatgcccgcg cggccgccga
     3121 ggaagcgcgc tggaacatgc gggtgacgct gagccttcgc aatcactacg agcgcgtgga
     3181 ggacggccgc gcgatcccgg tgccggcgca gaaggagccc gacgtggacg atctgctgga
     3241 ccgctacctg gtcatcggca cgcccgagac ctgcatccac cagatcgagc ggctgcgacg
     3301 cgacgtcggc atcacccact tcaactgcag cttctggttc ggcgacctcg agcagggccg
     3361 cgtcctgcgg tcgatggagc tcttcgcgcg cgaggtcatg cccgcgttcg cgtagcggag
     3421 gcccctcacc ccagccctct ccccgacggg gagagggagc catcgagaca accccacccc
     3481 cgaggagtca agagaaccct ctcccctctg gggagcaggg tgaggggtcg agcgccccac
     3541 gcctgtgcta ttctcgccgg cgcatggcag acaaggggcc gcgccacgca tggccgggcg
     3601 cgtcattggc gcgcgtgccg tactgggtct accaggacga cgcgaactac cgcgccgagc
     3661 tgcgccgcat cttcgagggg ccggtctgga actacctctg cctcgcctcc gacctcccga
     3721 aagcgggtga ctaccgcacc accttcctcg gcgagatgcc ggtcatcgtg gtccggggcg
     3781 cggaaggcca gatccacgcc ttcgagaacc gctgcgccca tcgcggcgcc ctgatcgcgc
     3841 tggacgacag cggatccacc cgcggccact tccagtgcgt ctatcacgcg tggacctacg
     3901 atctcgaggg ccgcctggtc ggcatc
//
LOCUS       DASUFA010000206        13640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1173600, whole genome shotgun sequence.
ACCESSION   DASUFA010000206 DASUFA010000000
VERSION     DASUFA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173600"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..488
                     /locus_tag="VFX87_07490"
     CDS             42..488
                     /locus_tag="VFX87_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX4994794.1"
                     /translation="MSASTYLPAGMPRPVPEPDGLDRPYWEGTRAGELRIQRCRACRG
                     WQWGPEWICHRCLAFDLEWVTVAPHGRIYSWERAWHPVHPALKDHGPYIVVLVELPEA
                     GGVRMLGNLLGDPRQAVQIGAPVEAVFEPHDEAAPPFTLVQWRMTP"
     gene            485..1693
                     /locus_tag="VFX87_07495"
     CDS             485..1693
                     /locus_tag="VFX87_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994795.1"
                     /translation="MTPTDIVVNEPNQWRLTTPGHAGWPRSARPGDPRKYFMVSADCH
                     ANEPYDLWATRIGEKYRARVPRVITDGNGVKWRVSEGHRPDRLRTEALEGEDGLRQRV
                     GADPVERLADMDRDGIDIEVIFPNKGLAMWATPDPVFAMAQCRVYNDWAHETFGRFRD
                     RMAPAAALATGDLEGSIAEVQRVAKLGFRVLTLPCKPIWGGHDVDHPNYNLPMFDPLW
                     SAIQDANLPVTFHVSTGRDPRAARGNGGAVINYVSHSLSPTIEPVANLCASGVFERFP
                     RLRVATIEAGIGWVAWFLSAMDEAYRKHHFWVRPKLKRLPSEYYREHCWASFQEDPAG
                     LALAEPMHLADRFMWGNDYPHHEGTWPHSAEAIERTMGHLSEAARANVLGLNAARFFG
                     FAVPAGSRLA"
     gene            1690..2520
                     /locus_tag="VFX87_07500"
     CDS             1690..2520
                     /locus_tag="VFX87_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX4994796.1"
                     /translation="MSPGAVRERHGAATGAHRAAGRVMDILELVVRTREGLALRELSA
                     QLEAPKSSLLPLLRTLTARGYLEQGALGEYRLGPKALELGMGSAAHRAWPEIARPALR
                     ELMQRTGETVFLGALGGDGTAVVFVDKVESEQVIRYTAGVGDRRALHATSSGKVILAF
                     LPAPERERILRALPLKRYTDRTVTSLPALRAALEEVRQTGVCVNLDELALGAAGIAAP
                     IFDRDGRVAGACAIGGPTDRVRPRLKPLAAEVRAAARAISALLGQRIDSPSASRRDQP
                     "
     gene            2546..3451
                     /locus_tag="VFX87_07505"
     CDS             2546..3451
                     /locus_tag="VFX87_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994797.1"
                     /translation="MATVFEKTEDVARVMDECGYDAIWLAEHHFQREGYECLPNILML
                     AVHLAQVTPRIRIGCGFNIAPMWHPLRLAEDFATADILTRGRTIFGVGRGYHSREVET
                     FGAPILDQEANRELFEEQVEIIFKAFDQESFSHQGKHYTLPPAVPYRGYALREITLVP
                     RPIHRPVECWQPIVSAKPRALDFMVRHRIKGVIGGGAATMAEGPVHGYREAAARAGRD
                     LALGEDLCLGIFFHLAETREKAIREITPYYEEHVKMFAPLGKDLESRFPGLQHVNMSI
                     SMGTPKPVMIEQLRWLAKEVMPKFA"
     gene            complement(3566..4216)
                     /locus_tag="VFX87_07510"
     CDS             complement(3566..4216)
                     /locus_tag="VFX87_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4994798.1"
                     /translation="MPPGLAAVVVQTQALFTILFAALALGERPTRREWTGTAVALAGL
                     ALIALTVGRDLTAAGLILCALSAVSWGVGNVLVKRLPPVDMLRLMVWLSVIPPLPSLA
                     LSLALDGPYALGTALAASSWLGRAASLYIGLIATVLGYAIWGGLLRRYPAATVTPFAL
                     LIPFVAAYASSLAFGERFGPLRLAGMGLVLLGLAVIVAPGVLAVTSWARRSRQLSR"
     gene            4564..5943
                     /locus_tag="VFX87_07515"
     CDS             4564..5943
                     /locus_tag="VFX87_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4994799.1"
                     /translation="MATRSLKIDHARYLLTVDRQRRIIQDGAILVEDGRITRVGKADE
                     LADARADRIIDGRHLVITPGFFNGHMHISYAHPVRGIFPDEQASPLLHVFNLQSAMTE
                     DEEYHATLLGLVELVRNGTVCFLDPGSTQFPDACLQAYEDAGIRVVLGEAVTDQEAPW
                     KLPRYPGPEAVARTRSFIEKWNGRLDGRIRAWAMPFSYETCSAGLLQALKRVVDEHRT
                     GLTLHHNSGTKARQDAVARYGHSPTAYLQSIGVMGPNVLLAHCLGLDETEIDAVAQTG
                     TAVAMCPVTAAKGGRGVPEHGRMPEFLARGVKVALGCDSPNNSNHLDMVRTMNMAAIQ
                     YKDARQDTRQVPAETALEMATIQGAHAMGLGDELGSIEVGKKADLVCFDTLRPEWQAL
                     WNPVNNLVYNADGRSVHTVVIGGRVVVDAYRQSFVDEPRLFARVQEIGERLQARTGVS
                     FPRSRWPMS"
     gene            5964..6416
                     /locus_tag="VFX87_07520"
     CDS             5964..6416
                     /locus_tag="VFX87_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX4994800.1"
                     /translation="MAKRQTGRSGWLEHSARVVTVWTGSSQVFAGAVGVIIAWALLGP
                     VFHFSDTWQLVINTGTTIITFLMVFLIQRSQNKDSLAIHLKLNEIVAVLQGASNRLIN
                     VEDLTEQEVATLHRHYQTLAQIAARDTTLTASHSVEEAETRAARKRVR"
     gene            6604..6978
                     /locus_tag="VFX87_07525"
     CDS             6604..6978
                     /locus_tag="VFX87_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4994801.1"
                     /translation="MSEPRILVVEDETNVRELEAEILREEWEHVDTAVDGTDALRLLA
                     ENVYSAIVSDLAMPKMDGRGLFHEVERRYPASQSRLVFVTGYAEAELYDEFLRQSGVP
                     VLAKPFTIADLVGAVQRAFSLA"
     gene            6993..7661
                     /locus_tag="VFX87_07530"
     CDS             6993..7661
                     /locus_tag="VFX87_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4994802.1"
                     /translation="MPRVEPSAQRYRELVEQSRGLICIHDLAGVLLSVNRAAAERLGY
                     TADELVGRSLGDLLAPTVRHLFPEYLGRIQQEATDEGLMLVVTRGGDERIWTYSNVRG
                     REPGGADYVLGHAHDITDIKRSDARAGEAEALRSVGQLALATAHEINNPLTVIMASLQ
                     LMVARGQVPESAAAYVQRAIKSAEQIRDTVRNMSRITRLEMSRQAPQLPAVLDLTKSS
                     APRD"
     gene            complement(7779..8690)
                     /locus_tag="VFX87_07535"
     CDS             complement(7779..8690)
                     /locus_tag="VFX87_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4994803.1"
                     /translation="MDERSAGRSRGPHLIWFALSLGAALTQAVQFAVVKGRAREIPPL
                     VIVAWTQGVACAAWVVFFLVSGHAFVPPGAAWPAIAASSLLVIGMSGLLARASARGDI
                     SIVGPILALSPIFTILPDAALTGALPSALGWVGLTLSLAGTVSLSGPGAVGLLGRVRA
                     LFARHDALDALGAAFFLGFLAAVDRWAALAIGPPSYLVSSHGTTAVLTTLIVLLTNPH
                     GLAASVAPRNLVTLVSHGLLGVAGTGMQTHALTMAPAAYVNAIRRMSAVLAVLLGRTL
                     FGEPDLARRLTAALLACLGAACLLLAK"
     gene            8831..9844
                     /locus_tag="VFX87_07540"
     CDS             8831..9844
                     /locus_tag="VFX87_07540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994804.1"
                     /translation="MARASFGLILANRAVVLGAIQARDLIELSQQAETSGAFDTLWVG
                     DSLLAKPRLEAVALLSALAGATSRLKLAVGCLATFVHRHPLLFAQQWASLDVLSGGRA
                     WLAVCLGGPDEQSAAQALEHKVMGVRSSERVGRMEEGIQILRKVWSGEKVSHQGAFYQ
                     FEGVKIEPSPVQRPCPIWIASNPTGLTWKGGGSASDAAVERGFRRVARYADGWMTNKV
                     SPAQFREQWGRIATMAREEGRNPDALGSALYHNININEDRQQALEESKSFLDTYYTSK
                     FSPAFVEGWTVAGSPARCIAELRDYAAAGLSHIALRLTSWDQRGQLKRFLTEVAPALA
                     GGR"
     gene            9864..12032
                     /locus_tag="VFX87_07545"
     CDS             9864..12032
                     /locus_tag="VFX87_07545"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX4994805.1"
                     /translation="MRWSLTGGQTAAIALLVAALLIDAVLTLHHIREVATGVQWVSHT
                     HEVLGQLEQVISTLKDAETGQRGYLLTGERAYLEPYEQAVPRLRGQFDQLRQLTADNA
                     PQTAHVLKLEQLTAEQLAVLRQGLDRFQAEPDKNRALMLSRQYLLQGEGKRLMDLIRD
                     EIYGMQQAERALLARRDAASRANARTALTSTMVGLALGLVLIAIVITLVGRNLVAREQ
                     ASDVLHAERERFRTTLTSIGDAVVVTDAQGRITLLNPVAQALTGWSGEALGQPLETVF
                     RIVNEATRETVENPVSKVIRLGTIVGLANHTVLIAKDGSALPIDDSGAPIRDTRGRIV
                     GVVLVFRDITERRGSERALEDADRRKDEFLAMLAHELRNPLAPIRNAAHTLALLGPGD
                     ERVRWVSGVIERQVGLMTRLVDDLLDVSRITSGKITLQRTTVSVRAVLDQAVEAARPP
                     AESRRQALEVDVPEDAGWVDGDPARLAQAVGNLLDNAIKYTDDGGRIRLRARVEADEV
                     VIVVEDTGAGIDPDLLPHVFDLFIQADRSLERKQGGLGLGLTLVRRLVEMHGGRVEAA
                     SAGPGLGSAFTIRLPRLAVAPTEPEAPVAPTEAAHPAGPARRILVVDDHQDSTDSLAL
                     LLRLRGHEVRTAHDGPSALAEIERDRPDVVFLDLGLPGMSGYDVARRVRTMNDPGPLR
                     LVALTGYGTDGDREKAREAGFDVHLAKPVDPRAVDALLAD"
     gene            complement(12079..12810)
                     /locus_tag="VFX87_07550"
     CDS             complement(12079..12810)
                     /locus_tag="VFX87_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4994806.1"
                     /translation="MRVVFWNIRAGGGGRAAALARQIGAWEADAVALAEFRGTPPSGE
                     LARDLARLGLPYQSTTADPARPGVNGLLLASRWPLRRLHLPCAPSEPCRWLLARVLAP
                     EPLALGVMHVPNRVGGRKYPFLDAVLTTARRRWTTPALLVGDTNSGRIGLDEEVPAFN
                     RIEDGWMRELDEAGWTDAYRHLRGAARAYTWYSPNGGNGFRIDQAFLNRALRPRLRRF
                     RYDWGRRPLGRPASDHAAMILDLER"
     gene            13099..13422
                     /locus_tag="VFX87_07555"
     CDS             13099..13422
                     /locus_tag="VFX87_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994807.1"
                     /translation="MIFQSAKGYLLFETGPAYTIMRAPIFGEVFVEWKSKEEWQARPP
                     DAGRWEIEVRQDVDALKGHRQSVVVLGRCTLTYWPWEVVRKLLLLNRRTTRRAALELP
                     PPPLR"
BASE COUNT         2096 a         4762 c         4965 g         1817 t
ORIGIN      
        1 acgcccgtct ctagcatgct cctgggcacg gaggccatgc gatgagcgcg agcacctatc
       61 tgcccgcggg gatgccgcgc cccgttcccg agcccgacgg gctcgaccgg ccctactggg
      121 agggcacgcg ggcgggggag ctgcggatcc agcgctgccg cgcgtgccgc ggctggcagt
      181 gggggccgga gtggatctgc catcgctgcc tggccttcga tctggaatgg gtgacggtgg
      241 ccccgcacgg ccgcatctat tcgtgggagc gcgcctggca tcccgtgcac ccggcgttga
      301 aggaccacgg gccctacatc gtggtgctgg tggagctgcc cgaggcgggc ggcgtgcgaa
      361 tgctcggcaa cctgctcgga gatccccggc aggccgttca gatcggcgcg ccggtcgagg
      421 cggtgttcga gccgcacgac gaggccgcgc cgccgttcac gctcgtccaa tggaggatga
      481 caccatgacg ccgaccgaca tcgtggtcaa cgagcccaac cagtggcggc tcacgacgcc
      541 cgggcacgcg ggctggccgc gcagcgcccg ccccggcgat ccgcggaagt acttcatggt
      601 ctcggcggac tgccacgcca acgagcccta cgatctctgg gcgacgcgga tcggcgagaa
      661 gtaccgggcg cgcgtgcctc gggtcatcac cgacgggaac ggggtgaagt ggcgcgtctc
      721 cgagggccat cgcccggacc ggctgcgcac cgaggccctc gagggcgagg acgggcttcg
      781 ccagcgggtg ggcgccgatc cggtcgagcg cctggccgac atggaccggg acggcatcga
      841 catcgaggtg atcttcccca acaagggcct cgcgatgtgg gccacgccgg atccggtctt
      901 cgccatggcc cagtgccgcg tctacaacga ctgggcgcac gagaccttcg ggcgcttccg
      961 cgaccgcatg gccccggcgg ccgcgctggc caccggcgat ctggagggct ccatcgccga
     1021 ggtgcagcgc gtcgcgaagc tcggcttccg cgtgctcacg ctgccgtgca agccgatctg
     1081 gggcgggcac gacgtggatc acccgaacta caacctgccg atgttcgacc cgctctggag
     1141 cgcgatccag gacgcgaacc tgccggtgac cttccacgtc tcgaccgggc gcgatccacg
     1201 cgccgcgcgg ggcaacggcg gcgcggtgat caactacgtc tcgcactcgc tctcgcccac
     1261 catcgagccg gtggccaatc tctgcgcctc cggcgtcttc gagcgattcc cccgcctgcg
     1321 cgtggccacc atcgaggcgg gcatcggctg ggtcgcgtgg ttcctgagtg cgatggacga
     1381 ggcctaccgc aagcaccact tctgggtgcg gcccaagctc aagcggctgc cgagcgaata
     1441 ctaccgcgag cactgctggg cctccttcca ggaggacccg gccgggctcg cgctcgccga
     1501 gcccatgcac ctggccgacc gcttcatgtg gggcaacgac tacccgcatc acgagggcac
     1561 ctggccgcac tcggcggagg ccatcgagcg caccatgggc catctgtcgg aggccgcgcg
     1621 ggccaacgtg ctcggcctca acgcggcgcg cttcttcggc ttcgcggtgc cggcggggtc
     1681 caggctcgcg tgagtcccgg agcggtccgg gagcgtcacg gcgcggcgac gggcgcccat
     1741 cgcgcagccg ggcgcgtgat ggacatcctc gagctggtcg tccgcacgcg cgaggggctg
     1801 gccctgcgcg agctgagcgc tcagctcgag gcgcccaaga gcagcctgct cccgctgctg
     1861 cgcaccctga ccgcccgggg ctacctcgag cagggcgcgc tcggggagta ccggctcggc
     1921 ccgaaggcgc tggagctggg gatgggctcg gccgcccacc gcgcgtggcc cgagatcgcc
     1981 cggcccgcgc tgcgggagct catgcagcgc accggggaga ccgtattcct gggcgccctc
     2041 ggcggcgacg ggaccgcggt ggtcttcgtg gacaaggtcg agagcgagca ggtcatccgc
     2101 tacaccgcgg gggtgggcga ccggcgggcc ctgcacgcca cctcgagcgg caaggtgatc
     2161 ctggccttcc tccccgcgcc ggagcgcgag cggatcctcc gcgcgctccc gctcaagcgc
     2221 tacaccgacc gcaccgtgac gagcctgccc gcgctgcgcg ccgcgctcga ggaggtgcgc
     2281 cagaccgggg tctgcgtgaa cctcgacgag ctggcgctgg gcgccgcggg catcgcggcc
     2341 ccgatcttcg accgtgacgg ccgcgtggcc ggcgcctgcg cgatcggcgg gcccaccgac
     2401 cgcgtccgcc cgcggctcaa gccgctggcc gcggaggtgc gggccgccgc ccgggccatc
     2461 tcggcgctcc tgggccagcg catcgattcg ccgtccgcgt cccgaaggga tcagccgtga
     2521 cgagcgacac tactcgaacg agcacctggc cactgtcttc gagaagacgg aagacgtggc
     2581 ccgggtgatg gacgagtgcg ggtacgacgc gatctggctg gccgagcatc acttccagcg
     2641 cgagggctac gagtgcctgc ccaatatcct catgctggcg gtgcacctgg cccaggtcac
     2701 cccgcggatc aggatcgggt gcgggttcaa cattgccccg atgtggcacc ccctgcgcct
     2761 ggccgaggat ttcgcgaccg ccgacatcct cacccgcggc cggacgatct tcggcgtggg
     2821 ccggggctac cacagccggg aggtcgagac cttcggcgcg ccgatcctcg accaggaggc
     2881 caaccgcgag ctgttcgagg agcaggtgga gatcatcttc aaggccttcg accaggagtc
     2941 gttctcgcat cagggcaagc actacacgct gccgcccgcg gtgccctacc gcggctacgc
     3001 gctgcgcgag atcaccctgg tgccgcgccc catccaccgg ccggtggagt gctggcagcc
     3061 catcgtgagc gcgaagccgc gggcgctcga cttcatggtg cgccaccgga tcaagggcgt
     3121 gatcgggggc ggggccgcga cgatggcgga gggcccggtg cacggctacc gggaggcggc
     3181 ggcccgcgcg ggacgcgatc tcgcgctcgg tgaggatctc tgcctcggta tcttcttcca
     3241 cctggccgag acgcgggaga aggcgatccg cgagatcacc ccgtactacg aggagcacgt
     3301 gaagatgttc gcgccgctcg ggaaagacct cgagtcgcgc ttcccaggcc tgcagcacgt
     3361 caacatgagc atcagcatgg gcacgcccaa gccggtgatg atcgagcagc tccgctggct
     3421 cgcgaaggag gtcatgccga agttcgcgta gcaagacgcg agaggcaacc cacgtgccac
     3481 ccgccgggcg gcagcagaat tcggatcatg ccctacgcgc cggtgttcgg ttccggcggg
     3541 gccgggagct tcttcccgga agggtttacc ggctcagctg tcgggaacgg cgggcccagc
     3601 tcgtcacggc cagcaccccc ggcgccacga tcaccgcgag tccgagcagg acgagcccca
     3661 tgcccgcgag ccggagcggg ccgaagcgct cgccgaacgc gagcgaggag gcgtaggccg
     3721 cgacgaaggg gatcaggagc gcgaacgggg tcacggtcgc ggcggggtag cggcgcagta
     3781 gcccgcccca gatcgcatag ccgaggacgg tggcgatcag gccgatatac agcgaggcgg
     3841 cgcggccgag ccatgagctg gccgcgagcg ccgtcccgag cgcgtacggc ccatcgagtg
     3901 ccagggacag cgcgagcgac gggagcggcg ggatcacgct cagccagacc atgagccgca
     3961 gcatgtcgac cggcggcagt cgcttgacga gcacgttgcc gacgccccag ctcaccgcag
     4021 acagggcgca caggatgagc cccgccgccg tcaggtcgcg gccgacggtg agggcgatca
     4081 gcgcgaggcc cgcgagcgcc accgcggtgc cggtccactc gcggcgggtg gggcgctcgc
     4141 ccagggccag cgcggcaaag aggatggtga agagcgcctg ggtctggaca acgaccgcgg
     4201 ccaggccggg tggcatcccc aacgcgatcc gaagaactgc agaaggaact ggcccaggaa
     4261 cagggtgagg ccgaccggga cgagggtccg ccagggcacg cgcggtcgcg cgagcaccag
     4321 ggcgggcgcg gccgcgatga ggaagcgcag cgcggtgagc tggggcgggg agaaatcgtc
     4381 gagcgcccac cgggtggcga cgaaggtgag gccccagatg acgaccacca agagcaccag
     4441 ggcgagatgg gtgggcgcca tgtcgcccga ccatagcacc gcggggcgtc cgatggacaa
     4501 ggccccgccg cgaagcgtag actccgggcg gctcatcatc cccgttccgg gaggcggcgt
     4561 cccatggcta cgcggagtct caagatcgat cacgcgcggt acctcctgac cgtcgatcgc
     4621 cagcgccgca tcatccagga cggcgccatc ctggtggagg acggccgcat cacccgggtc
     4681 ggcaaggcgg acgagctggc cgacgcgcgg gccgaccgca tcatcgacgg gcgccatctc
     4741 gtgatcaccc ccggattctt caacgggcac atgcacatca gctacgcgca tccggtgcgc
     4801 ggaatctttc ccgacgagca ggcgagcccg ctgctccacg tcttcaatct ccaatcggcg
     4861 atgaccgagg atgaggagta ccacgccacc ctgctcggcc tggtcgagct ggtccgcaac
     4921 gggacggtgt gcttcctcga cccggggagc acgcagttcc ccgacgcgtg cctgcaggcc
     4981 tacgaggacg cgggcatccg cgtcgtgctg ggcgaggcgg tgaccgatca ggaggcaccg
     5041 tggaagctgc cgcgctaccc ggggccggaa gcggtggccc gtacccggtc gttcatcgag
     5101 aagtggaacg gccggctcga cgggcggatc cgggcgtggg cgatgccgtt ctcgtacgag
     5161 acgtgcagcg cgggcctgct gcaggcgctc aagcgggtgg tggacgagca ccgcaccggc
     5221 ctcacgctcc accacaacag cgggaccaag gcgcgccagg acgcggtggc ccggtacggg
     5281 cacagcccga ccgcctacct ccagtccatc ggggtgatgg ggcccaacgt gctgctggcc
     5341 cactgcctgg gcctcgacga gacggagatc gacgcggtcg cgcagaccgg caccgcggtc
     5401 gcgatgtgcc cggtcaccgc ggccaagggc ggccgcggcg tccccgagca cggccgcatg
     5461 cccgagttcc tcgcccgcgg tgtgaaggtc gcgctcggct gcgactcgcc caataactcg
     5521 aaccacctcg acatggtccg caccatgaac atggcggcca tccagtacaa ggacgcacgc
     5581 caggacacgc gccaggtccc cgcggagacc gcgctcgaga tggcgacgat ccagggcgcg
     5641 cacgcgatgg ggctgggcga cgagctgggg tcgatcgagg tcgggaagaa ggccgatctc
     5701 gtctgcttcg acaccctgcg cccggaatgg caggcgctct ggaacccggt caacaatctg
     5761 gtctacaacg cggatggccg cagcgtgcac accgtcgtga tcggcgggag ggtggtcgtg
     5821 gatgcctacc ggcagagctt cgtggacgag ccgcgtctct tcgcccgcgt gcaggagatc
     5881 ggcgagcggc tgcaggcccg caccggcgtc tcgttcccgc gctcgcgctg gccgatgagc
     5941 tgagggcccg ggaggacttc acgatggcca agcggcagac cggcaggagc gggtggctgg
     6001 agcattcggc gcgggtggtc acggtctgga cgggcagctc gcaggtcttc gcgggcgcgg
     6061 tcggcgtcat catcgcctgg gcgctgctcg ggccggtctt ccacttctcg gatacctggc
     6121 agctcgtcat caataccggt accaccatca tcacctttct catggtgttc ctgatccagc
     6181 gctcccagaa caaggactcg ctggcgatcc atctcaagct caacgagatc gtcgcggtgc
     6241 tgcagggcgc gagcaatcgg ctgatcaacg tcgaggatct caccgagcag gaggtggcga
     6301 cgctgcaccg gcactaccag acgctcgccc agattgccgc gcgcgacacc acgctcaccg
     6361 cgtcgcactc cgttgaggag gccgagaccc gcgccgcccg caagcgcgtc cgctgaccgg
     6421 gaagccaccg ccgggtgact cacggcgcgc ccgcactgac acttcccagc agtctcccgc
     6481 tcccgcgcat ctcgtaactc cttgatttgt gtggcccggc tcgtggtgca cttcttgcat
     6541 cagaattgcg ccaggccgag gctcgtgagc ccggccattt ccggtatcga gaggagaagg
     6601 cccgtgtccg aaccgcgcat tctggtggtc gaggatgaga cgaacgtccg ggagctggag
     6661 gcggagatcc tgcgggagga gtgggagcac gtggacaccg cggtggacgg cacggacgcg
     6721 ctgcggctcc tggccgagaa cgtctactcg gcgatcgtga gcgatctcgc gatgccgaag
     6781 atggatgggc gcgggctctt ccacgaggtc gagcgccgct acccggcctc gcagagccgc
     6841 ctcgtgttcg tcaccggata cgcggaggcc gagctgtacg acgagttcct gcggcagagc
     6901 ggcgtgcccg tgctcgccaa gcccttcacc atcgccgacc tggtcggcgc ggtgcagcgg
     6961 gccttctcgc tggcctgagc ggaggcccgc ccgtgccccg cgtcgagccg tccgcgcagc
     7021 gttatcgcga gctggtggag cagagccgcg gcctgatctg catccacgat ctcgccggcg
     7081 tcctgctctc ggtcaatcgc gccgccgccg agcgcctcgg ctacaccgcg gacgagctgg
     7141 tgggccgcag cctgggcgac ctgctcgccc ccaccgtccg gcatctcttc cccgagtacc
     7201 tcggccggat ccagcaggag gccaccgacg aggggctcat gctcgtggtg acacggggcg
     7261 gagacgagcg catctggacg tacagcaacg tgcggggccg ggagccgggc ggggcggact
     7321 acgtgctggg ccacgcccac gacatcaccg acatcaagcg gtccgacgcg cgggcgggcg
     7381 aggcggaggc cctgcgctcg gtcggtcagc tcgcgctggc gaccgcgcac gagatcaaca
     7441 acccgctcac cgtgatcatg gcgagcctcc aactcatggt ggcgcggggg caggtgcccg
     7501 agtcggcggc cgcatacgtg cagcgcgcga tcaagtcggc cgagcagatc cgcgacaccg
     7561 tccggaacat gtcccggatc acccggctcg agatgtcccg ccaggccccg cagcttcccg
     7621 ccgtcctcga tctcaccaag tccagcgccc ctcgggactg aaccgtcgcc ggcggggtct
     7681 tgccgccggg cccctggggg tcaggtctta cattccgaca tttgccagcg atcgaggccg
     7741 ccctgggcgc cggaatgtcg gaatgtaaga cctgacccct acttggcgag gaggaggcag
     7801 gcggcgccga ggcaggcgag gagagcggcg gtgaggcggc gggcgaggtc gggctcgccg
     7861 aagagcgtgc ggcccagaag gacggccagc accgccgaca tccggcggat cgcgttcacg
     7921 tacgccgcgg gcgccatcgt gagcgcgtgc gtctgcatcc cggtgccggc gacgccgagg
     7981 agcccgtggc tcaccagggt gaccaggttg cgcggcgcaa cgctggccgc cagcccgtgc
     8041 gggttggtca gcagcacgat caaggtggtg agtaccgcgg tcgtgccgtg cgaggacacg
     8101 aggtagctgg gcggcccgat ggccagcgcg gcccagcgat ccaccgcggc caggaagccc
     8161 aggaagaacg cggcgccgag cgcgtcgagg gcatcgtgcc gcgcgaagag ggcgcgaacg
     8221 cgcccgagca ggcccacggc gccgggcccc gagaggctca cggtgccggc cagggacagg
     8281 gtcagcccga cccagccgag cgcgctgggc agggcacccg tgagcgcggc gtccggcagg
     8341 atcgtgaaga tcgggctcag ggccaggatc ggcccgacga tcgagatgtc gccccgcgcg
     8401 ctggcccgcg cgagcaggcc gctcatcccg atcacgagga gggacgaggc ggcgatggcg
     8461 ggccaggcgg cgccgggcgg cacgaacgcg tggcccgaga ccaggaagaa gaccacccat
     8521 gccgcgcagg cgacgccctg agtccacgcg acgatcacca gcggggggat ctcccgcgcg
     8581 cggcccttga cgacggcgaa ctgcacggcc tgggtgagcg ccgcgccgag cgacagcgcg
     8641 aaccagatca ggtgagggcc acgggaacgg ccggctgagc gctcatcgat cctccgtccg
     8701 tcggagaccg tagccgaaga cccgggcgcg ggtcaatcgg gtagaagcca ccggggcgtc
     8761 ccgcggccgc cgggcgcccg cgcttgacgt actccgccgc gacccccaag attggcggcg
     8821 ggaggggacc atggcgcgag cgtccttcgg attgatcctg gcgaatcggg cggtggtgct
     8881 gggcgcgatc caggcccgcg acctgatcga gctgtcgcag caggccgaga cgtcgggcgc
     8941 cttcgacaca ctctgggtcg gcgacagtct cctggccaag ccgcgcctcg aggcggtggc
     9001 gctgctctcc gcgctggccg gcgcgacgtc gcgcctcaag ctcgcggtcg gctgcctcgc
     9061 caccttcgtg caccgccacc cgctgctctt cgcgcagcag tgggcgagcc tcgacgtgct
     9121 ctcgggcggg cgggcgtggc tggcggtctg tctcggcggg cccgacgagc agagcgccgc
     9181 ccaggcgctc gagcacaagg tcatgggcgt gcgctcgagc gagcgggtgg gccgcatgga
     9241 agagggtatc cagatcctcc ggaaggtctg gagcggcgag aaggtctcgc accagggcgc
     9301 cttctaccag ttcgagggcg tcaagatcga gccgagcccg gtgcagcggc cgtgcccgat
     9361 ctggatcgcg agcaacccga ccggcctcac ctggaagggc gggggcagcg cctccgacgc
     9421 ggcggtcgag cgcggcttcc gccgggtggc gcgttacgcg gacggctgga tgaccaacaa
     9481 ggtgagcccg gcccagttcc gcgagcagtg ggggcgcatc gcgacgatgg cccgcgagga
     9541 gggccgcaac ccggatgccc tcggcagcgc gctctaccac aacatcaaca tcaacgagga
     9601 ccggcagcag gcgctcgagg agagcaagag cttcctcgac acctactaca catccaagtt
     9661 ctcgcccgcc ttcgtggaag ggtggacggt ggcggggagc ccggcccggt gcatcgccga
     9721 gctgcgcgac tacgccgcgg cgggtctctc gcacatcgcg ctccggctca cgtcgtggga
     9781 ccagcgcggt cagctcaagc ggttcctgac cgaggtggcg cccgccctcg cgggcggccg
     9841 ctgacgcggg cgaagactgc acgatgcgct ggagcttgac cggcgggcag accgccgcca
     9901 tcgccctgct ggtcgcggcg ctcctgatcg acgcggtcct cacgctgcac catatccgcg
     9961 aggtcgccac cggcgtgcag tgggtgtccc acacccacga ggtgctgggc cagctcgagc
    10021 aggtcatctc caccctcaag gacgcggaga ccggccagcg cggttaccta ctcaccggcg
    10081 agcgggccta cctcgagccc tacgagcagg cggtgccgcg gctccgcggg cagttcgacc
    10141 agctccggca gctcaccgcg gacaacgccc cgcagaccgc gcacgtcctc aagctcgagc
    10201 agctcaccgc cgagcagctg gcggtgctcc ggcaggggct cgaccgcttc caggccgagc
    10261 ccgacaagaa ccgggcgctc atgctctccc gccagtacct cctccagggc gagggcaagc
    10321 gcttgatgga cctgatccgc gacgagatct acggcatgca gcaggccgag cgcgcgctgc
    10381 tcgcccgacg ggacgcggcc tcgcgcgcca acgcccgcac cgcgctgacc agcacgatgg
    10441 tcgggctcgc gctggggctc gtgctgatcg cgatagtcat cacgctcgtc gggcgtaacc
    10501 tggttgcccg cgagcaggcc tccgacgtgc tgcacgccga gcgcgagcgc ttccgcacca
    10561 cgctcaccag catcggcgac gccgtcgtgg tgaccgacgc gcaggggcgg atcaccctgc
    10621 tgaacccggt ggcgcaggct ctcaccggct ggagcggcga ggcgctcggc cagcccctgg
    10681 agacggtgtt ccgcatcgtc aacgaggcga cgcgcgagac ggtggagaac ccggtgagca
    10741 aggtgatccg cctcgggacc atcgtgggtc tcgccaacca caccgtgctg atcgccaagg
    10801 atggcagcgc gctgcccatc gacgacagcg gtgcgccgat ccgcgacacc cgcggccgta
    10861 tcgtgggcgt ggtgctggtc ttccgcgaca tcaccgagcg gcggggctcg gagcgcgcgc
    10921 tggaggacgc cgaccggcgc aaggacgagt tcctcgccat gctggcccac gagctgcgca
    10981 acccgctcgc gcccatccgc aacgcggccc acaccctggc gctgctcggc cccggcgacg
    11041 agcgggtgcg ctgggtctcg ggcgtcatcg agcggcaggt ggggctcatg acccggctcg
    11101 tggacgatct gctcgacgtc tcccgcatca cgtcgggcaa gatcacgctg cagcgcacga
    11161 cggtctcggt gcgcgcggtc ctcgaccagg cggtggaggc ggcgcgcccg cccgcggaga
    11221 gccggcggca ggcgctcgag gtggacgtgc ccgaggacgc gggctgggtg gacggcgatc
    11281 ccgcgcggct ggcccaggcg gtcggcaacc tcctcgacaa cgccatcaag tacaccgatg
    11341 acggcgggcg gatccggctc cgcgcgcggg tcgaggcgga cgaggtcgtg atcgtggtcg
    11401 aggacaccgg ggccggcatc gatccggatc tcctgccgca cgtgttcgat ctgttcatcc
    11461 aggccgatcg ctcgctcgag cgcaagcagg gcgggctcgg cctcggtctg accctggtcc
    11521 gccgcctggt ggagatgcac ggtgggcgcg tggaggcggc gagcgcgggc ccgggtctcg
    11581 gcagcgcctt caccattcgc ctgccgcgcc tggccgtggc gcccacggag cccgaggctc
    11641 ccgtggcgcc cacggaggcg gcccacccgg cgggcccggc ccgtcgcatc ctggtggtgg
    11701 acgaccacca ggactccacc gattcgctgg ccctcctcct gcgcctgcgc ggccacgagg
    11761 tgcgcactgc gcacgacgga ccgagcgcgc tcgccgagat cgagcgcgac cgtcccgacg
    11821 tggtgttcct cgacctgggg ctgcccggga tgagcggcta cgacgtggcg cgccgggtgc
    11881 gcacgatgaa cgacccgggc ccgctgcggc tggtggcgct caccggttac gggaccgacg
    11941 gagatcgcga gaaggcacgc gaggccggct tcgacgtcca cctggccaag ccggtggatc
    12001 cgcgggccgt cgacgcgctg ctggccgact gaaccggtct gtcccccagg ccgcggcgtg
    12061 ctagccggtc ctccgccgct agcgttcgag gtcgaggatc atggccgcgt ggtcgctggc
    12121 cgggcggccg aggggccggc gaccccaatc gtagcggaag cggcgtagcc ggggtcgtag
    12181 cgcgcggttg aggaaggcct ggtcgatccg gaagccgttg ccgccgttcg gggagtacca
    12241 ggtgtaggcg cgcgcggcgc cacgcaggtg gcggtaggcg tccgtccagc ctgcctcgtc
    12301 gagctcccgc atccagccgt cctcgatccg gttgaacgca gggacttctt cgtcgaggcc
    12361 gatgcggccg gagttcgtgt cgccgaccag cagggcgggg gtggtccagc gccgccgcgc
    12421 ggtggtgagg accgcgtcga ggaacggata cttgcgcccg ccaacgcggt tggggacatg
    12481 catcacgccc agggcgagcg gctccggcgc cagcacgcgg gccagcagcc agcggcacgg
    12541 ctcgctgggc gcgcagggga ggtggagccg gcgcagcggc cagcgcgagg ccagcagcag
    12601 gccgttgacc ccgggccgcg ccgggtccgc cgtcgtcgac tggtagggga gcccgagccg
    12661 ggccaggtcg cgggccagct ccccgctggg cggcgtgccg cggaactcgg ccagcgcgac
    12721 cgcgtccgct tcccaggcgc cgatctgccg agcgagggcg gcggcgcgcc cgccgccccc
    12781 cgcgcggatg ttccagaaga cgacccgcat ccggatctgc gctacttgtc gagatactgt
    12841 ggcactggat cctcctcccc aagctcgcgc tgggtcaggc ccggcgcgtt ccggtgctcc
    12901 ttgggtgagt cgtgcatgtg acgcgacaac cgtcacccat gggacagacc gaccgccggt
    12961 cacacacccc agacgagccc acggtacgcg agtttcgccc atgcgggccc gctggttatc
    13021 gcgcggctag cggtctcggg cctggttggc acagagcttg cccgtgaagt ggctcaggga
    13081 ctgaggagga cctctgcgat gatcttccaa agcgccaagg gttacctgct ctttgaaacg
    13141 gggcccgcgt acacgatcat gcgcgctccg atcttcgggg aagtgttcgt cgagtggaag
    13201 agcaaagaag agtggcaggc ccggcctccg gacgctggcc gctgggagat cgaagtacga
    13261 caagatgttg acgccttgaa aggccatcgt cagtccgtgg tcgtgctggg gcggtgcacg
    13321 ttgacgtact ggccgtggga ggtggtgcgg aaactgctcc ttctgaaccg gaggacgacc
    13381 cggcgagcgg cgcttgagct accgccgccc ccgctgcggt agaggcttcg cacgggacgc
    13441 cgcccgcaag ctccgggtcg aactagtcga gcggcgtgtg agggtgtcga tccccacgac
    13501 gagcgcgtcg gcgcgctgcc tgatcgcggt ctcgtacgcc gcccctagat cgagcgagag
    13561 tgtcgtgatg gggcgcgcag ttactacttg tcgagccaga cttcctggag ccggcagcag
    13621 ttgtaggtga cctggttcgg
//
LOCUS       DASUFA010000207        21182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_853128, whole genome shotgun sequence.
ACCESSION   DASUFA010000207 DASUFA010000000
VERSION     DASUFA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 21182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21182
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853128"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFX87_07560"
     CDS             <1..987
                     /locus_tag="VFX87_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX4994808.1"
                     /translation="WAPERAELDEIARFHSREYIEILRAVSAGDWVPHAARYGLGPGD
                     NPIFPGLWEAAQLGAGGSLLAARLVADGEVTRAFHFAGGLHHAMPGRASGFCSVNDAV
                     LAIMRLRQGGLRVAYVDIDAHHGDGVQFAFYDDPNVLTVSTHERGDRLFPGTGFVVEM
                     GEGAGLGYSVNVPLQPLTDDAVYHEAFEAVVPPLVTAFKPDALVIQLGIDSHRTDPLT
                     HLSLTVQGFTRAVKRLLSLAPRVVALGGGGYDLTNVARAWTAAWAAMNDVDLPPDLPR
                     SSHRDMQRLGLGTLSLNDPVEVLPPDTRRWAEEYARRQVGEIQDRIFPLHGL"
     gene            997..2451
                     /locus_tag="VFX87_07565"
     CDS             997..2451
                     /locus_tag="VFX87_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4994809.1"
                     /translation="MDTTPGASVVAPSAPLEAVAPDAPARAPIRIPLKVKLSLLITAL
                     VVLTVALVGLFLLRQQQQSLTAEMTKRGLTMAENFAAGAKTPLLTNDELTLGVLVTDA
                     MKDPDVAYVIVSDQDGNVLAHSDARAVKTAVVRPKELEPLKNRVLVQTYKTPQGRIID
                     FAVPLVYSGVPVGALYLGFSEEAITAALASARNQALLITLLMVLAGLGGAVGLAALLS
                     RPIFRLVQATGAIAAGNFNIEVPVASRDELGALTDSFNRMARSLREKEMIKRAFTRYV
                     AREVVEEILKDPEHLVLSGERRQVTVLFCDVRGFTPMSERLAPEEVVLLLNDFYTLMI
                     ETTFKYDGTLDKFLGDAVMAVFGAPMAHPDHSARAIRTALAMQEGITGLNERRVRDGK
                     EAISVGIGVSAGEAVAGTVGTEDRMEYTVIGDSVNLAARLESNAKPGQILISYRTYER
                     VRDLVDARPLGRIRVKGKEEEVEVYEVLGLMPVV"
     gene            2489..3544
                     /locus_tag="VFX87_07570"
     CDS             2489..3544
                     /locus_tag="VFX87_07570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4994810.1"
                     /translation="MKRRARGSGLLVSAAALWLAACATASAPAPGPAPAAPAPAATAP
                     ARPQPRPAAYQSEDFIVTFAQPGDTPETLAERYLGGAEKGWMIEDYTGLRAFAPGQEV
                     VIPRRPWNPAGVTPGGYQIVPILTYHNLGEQAKGRLVLAAASFREQMRYLKTNGYRVV
                     SLAEFVEFTRLNRQLPQRAVVLTFDDGYRAFKDLAYPVLKELGFTATLFIYTDWVGAG
                     RGALSWADLRELAAAGMDIQAHSKTHADLRRAQGETEAQYARRMQAELEQPQDLFNRN
                     LGRRSQILAYPYGRWEEGLLPKVKEHGYIAAFSVRRQGNASFVRPLAGHRSQIYSEMT
                     LDDFVKNLNVFQEENLR"
     gene            3541..4716
                     /locus_tag="VFX87_07575"
     CDS             3541..4716
                     /locus_tag="VFX87_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX4994811.1"
                     /translation="MTGAGRGWLVAAALGLALGGCASLSPSAPSEPAAGPAPIARDPL
                     AARHRQQAEALERDGQLRRAAEEWKIALAIAPGDARARESLRALQARIDQAVAERLDE
                     GRKALARGVQVEARRKFLAALALDPSNRAAFDALQSDTREVEFLTHTVRAGDTLTTLA
                     QRYYGDRSRGEVIWETNQLPPNPRLAPGTTLKIPEIPGVPFMHPEARRPAPAVAAIPT
                     PDVPRPGPTPGPAAPAAPSAKEEFTSEVNPLLAEAREALERSDYPDAIATVDKFLSGK
                     PGDREGLTLKKQALYGQAKSQMEARQYPESIQSLNQLARLQPDYEDVPSLLRQARTRI
                     IEQHYTKGVRFYREEKLKEAIAEWRVVLEMDPGNANARRNIDQAEKLLRGLEQRMKK"
     gene            4745..5341
                     /gene="pal"
                     /locus_tag="VFX87_07580"
     CDS             4745..5341
                     /gene="pal"
                     /locus_tag="VFX87_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009801867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX4994812.1"
                     /translation="MNRHVRTVATLLPIISLTLLLAGCPKRPAMTAATAPPPVPPAAV
                     APPTPAAPAPAPAPIAPAPVAPPTVAPAPAPAPPAPPKEYRSNAALNQIFFAFDKADI
                     RSGDAQILAASAAYLKANPNQLVLIEGHCDERGTSEYNLALGERRAKAAMNYLVSNGV
                     EASRITTISYGKERPGCTEKNEACWSKNRNDTFLTKER"
     gene            5404..6204
                     /locus_tag="VFX87_07585"
     CDS             5404..6204
                     /locus_tag="VFX87_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX4994813.1"
                     /translation="MPVQDLILDLAIRYGFQVLGAFVILGAGVAVAWWVGNAVERPLT
                     RMALEPPMRRLMVRVVRVVVLLFALVIALDKFGFQIAPLVAGIGVAGLGIGIALQGVL
                     GNVIAGLTIIFTKPFRVGEHVEVVGMKGDVHTIELFSTTLLHPDRSRIIIPNRKIVGE
                     ILHNFGNTRQIHLAVMVPHGSDLAAVLTLVREVVVSNPRVLADPDPVVGVAQLELAGV
                     RVGVNPWVRVPDVVAAEAELYRALADRFARSGIQVPLPKQEVRVLMSS"
     gene            6263..6550
                     /locus_tag="VFX87_07590"
     CDS             6263..6550
                     /locus_tag="VFX87_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994814.1"
                     /translation="MKTLPLAVLLLLASAGIAPAQTKSPGPLDLEYKQRAPALRPVPP
                     QKIEEDTTQAVRELDRQRTDRMLRAVQPGPSRRPDLDRDVTQGIQTRGLGR"
     gene            complement(6547..10794)
                     /locus_tag="VFX87_07595"
     CDS             complement(6547..10794)
                     /locus_tag="VFX87_07595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4994815.1"
                     /translation="MFHPVVESWFRARYSEPTPVQAGAWPLIADGQDVLLTAPTGSGK
                     TLAAFLACLDRLFREAVEGRLADRTAILYVSPLKALSNDVRRNLDEPLAELAAAAVAA
                     GLPAPEIRTAVRTGDTPAHERRLIGRRPPHVMVTTPESLFILLTAESSRRWLADVRTV
                     IVDEIHAVAGDKRGAHLALSLERLTELVATRGARLQRIGLSATVRPIQTAARLLVGAD
                     RRLPEIVNVGQRRDMDLAIEVLGDELGAVCTNEQWEEIYDRVAELARAHRSTLVFVNT
                     RRLVERVTRHLAERLGAERVAAHHSSLSRQRRFDAEQRLKAGQLSLVVATASLELGID
                     VGTVDLTCLIGSPRSIATALQRVGRSGHALLATPKGRLFPLTRDQLVECAALVRAARC
                     GEIDRLGLRTAPLDVLAQQIVAICACEEQDEDGLFDLCRRAAPYAALGREDFDQVVDM
                     LAEGIATRRGRYAARLHRDGVARRLKARRGARLAALTSGGAIPDNASYEVVLEPDETT
                     IGSLDEDFAIESMAGDVILLGNSSWRIRRIESGRVRVEDAGGAPSNIPFWLGEGPART
                     LELSAELSAVRQGVADRLHDPESAAAWLERETGLDRRGALLARDYIWAAKAALGAVPT
                     RETVVVERFFDEAGGMQLVVHAPFGGRINRAWGMALRKRLCHSFNFELQAAATDDGVL
                     LSLGPQHSFPLEAVFEMLHPSGIEELLIQAALQAPMFNTRWRWNASRALALLRWQNGR
                     KVPPYLQRMRAEDLLVAVFPAQLACQDNAEPGPIEVPDHPLVRETLRDCLTEAMDIDG
                     LRATLTALHAGEIKRITRETPEPSVFAHEILNANPYAFLDDAPLEERRARAVTLRRGL
                     PASVTDDVGRLDPAAITAVVNEAWPDLRSADELHDLLLDLGALPERDGEAWTGYLDEL
                     VAAGRAARLRRGSHVFWVPAERRSLAATVWPDSRFEPDLIEPPARRTPMWSGQDGALV
                     EVVRARLGLVGPSTTAALATALDVTPGQVDAALAAVEAEGGVLRGRFTPEAIAAGQVE
                     WCERRLLARIHRRTLDGLRRLIEPVSPAVLLRFLLSWQHARPATQQHGRDGLLRVIER
                     LQGFEAAAGVWERDLLPSRVAGYQPGWLDELCLSGEVAWGRLGLHESARHAGRRLSAT
                     VPVALLLRRDLAWLLAPRPAPGADAPDRTPAAASPIQPSGPARDVLAHLERAGASFVD
                     DIASGVRRLRIEVEEALCELVTAGLVTGDGFAGLRSLLHGEKRRRPRPGRPVAPRPSA
                     GTGRWARLLPPTTPATDDIVEAKARQYVRRYGVVFRDLLQREPDVPAWRDLLRVYRRL
                     EMRGDLRGGRIVGGFVGEQFAAPEALEALRAIRREEPSGQTVRISACDPLNLVGIITP
                     GPRVTAVPGHHVNYRDGVPVLEETRTRGERTGGRKRR"
     gene            11032..12039
                     /locus_tag="VFX87_07600"
     CDS             11032..12039
                     /locus_tag="VFX87_07600"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994816.1"
                     /translation="MDSSCSMPRRTFMAMMAGGLLTAPLAAEAQQARVPKIGVLLLFL
                     FGTPGQPSPLLDALRGGLRDLGYVEGQNLVIEYRWAEGQAQRLAELATDLVRLNVAVI
                     VTGGTPATLAAKAATTAIPVVMTNVGDPVGSNIVASLARPDANVTGLSLLETDLDGKR
                     IELLKEAVPHLTRLAILWSANDPGMTLAFGRVGMAAQALGLALQNLAVREPADFPRAF
                     DAAVAGRAEALFVTAQPFTNRHRAEILALVAKQRLPAMYTLRGFVDAGGLMAYGPSQT
                     DQYRRAATYVDRILKGAKPADLPVEQPTTFDLVINLKTAKALGLTMPPSLLQRADQVI
                     E"
     gene            12039..13031
                     /locus_tag="VFX87_07605"
     CDS             12039..13031
                     /locus_tag="VFX87_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994817.1"
                     /translation="MDRRSFLGTLAGGLLAAPLAAEGQQAARVARIGLLRPGAPTDPA
                     DRSSVGAFVAGLQEAGYTQGTNIRVETRYAHGEFDRLPALARELAALPVDAIVTSNPY
                     ATRAVRAATRTVPIVVALDYETDPVASAWIAGIARPGGNLTGLFLDQPAMAGKLLQFL
                     MEGVPRLATVSVLWDETIGRIQFDTMEKVAREKGLTIRSFGVKRSEDLADAFGGAARK
                     RADGLVVLTSPLLSLNRAQIGALALRHRLPGITLFTNFPAFGLLMAYGPNQPDAFRRA
                     ASDYVARILKGDRPADLPVQRPEKFDLVINLKTAKALGLTMPPSLLQRADQVIE"
     gene            13140..14405
                     /locus_tag="VFX87_07610"
     CDS             13140..14405
                     /locus_tag="VFX87_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX4994818.1"
                     /translation="MTASSETVIVGGGHAGLALSYHLGRLGRPHVILERGRVAERWRS
                     ERWDSLMFQFPNWSLRLPGQDYSGDDPDGFATRDEVIAFIERYRALVAAPVRTGVRVD
                     RVRPVDGGFRLETSEGDVDATNVVVATGPYQEPILPAVRQALPLGVVQMHASGYRNPA
                     QLPPGAVLVVGSGASGCQIVEDLLAAGRTVYLSVGRHRRYPRRYRGRDMFWWMERIGA
                     LDQTLEERPDARERPNPLVTGVGGGHDIDLRDYAAAGVTLLGHLRAVTGSRLYLADDL
                     EPLLAAGDESVGVFTRAVDAYIARAGLAAPAEPPPPPASPRTAPAPIRELDLAASGIT
                     SVVWATGFRRDFSWIDAPVFDERGEPIHRRGVTGCEGLYFLGLPWLHKLKSSVLCGVG
                     DDAAHLADHIAYDLIGARSPRAPRAPGRD"
     gene            14419..15522
                     /locus_tag="VFX87_07615"
     CDS             14419..15522
                     /locus_tag="VFX87_07615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994819.1"
                     /translation="MAIEFIGMIGVRPEGADGAAVHVIGGEIDMGWVREFAQAHERAG
                     FDKVLVGYTSTSADGFLVSAYAAAYTERLGYLLAHRPGFVAPTLAARKAATLDQLSGG
                     RLALHIITGGSDADQARDGDFVDHDTRYRRTDEYLGLMRRVWTEARPFDHAGEFYRVA
                     AAYSDVKPVQRPHVPLYFGGASEAAHRIGVRHCDVYMLWGEPLAAIRDRVATVRAAAA
                     EAGREPGAPRFSVSLRPIIAPTEAQAWGKARTILEQVQTKQGQRGVPQPEAVGARRLV
                     DFARQAEVHDKRLWTSIAAAVGGQGNTTALVGTPEQVAEALLDYYDLGVTTLLIRGFD
                     PLNDAIEYGRELIPLVKAEVARRERDRAAALAR"
     gene            15519..16100
                     /locus_tag="VFX87_07620"
     CDS             15519..16100
                     /locus_tag="VFX87_07620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX4994820.1"
                     /translation="MTRLLAVVGAVTPPGRLDAAVRFAADRATDRAGDRRDVSATVLS
                     LGEHRVSFADGRPLDRYTDDTARVVGAIAEADAVLLASPVYRASFTGALKNLLDLTPV
                     EALRAKPVGIVAMGATLHHYLGVDWQLRAVLAWFGALVVPTSVYLESGQFQDGKLADP
                     AARAALGDLVAALVAMAGHARDQAGPPPLAAGR"
     gene            complement(16129..16683)
                     /locus_tag="VFX87_07625"
     CDS             complement(16129..16683)
                     /locus_tag="VFX87_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(16450..16452),aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4994821.1"
                     /translation="MTARPLAASILIAAALWAGAAAAQPTAAPPAWEKLGVQAYESPR
                     PAPDFALPALDGRTVRLEDVRGKALLLFFWATWUPTCREELPSVNRLYEEFKGQGLEV
                     LLISFRESSDVVKRTVAERGYTAPVLLDASGDVTGRIYGVFGPPTAYLVDREGRLVGR
                     MVGARDWSGAAAREVIRGFVSTRR"
     gene            complement(16680..17006)
                     /locus_tag="VFX87_07630"
                     /pseudo
     CDS             complement(16680..17006)
                     /locus_tag="VFX87_07630"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(17019..17240)
                     /locus_tag="VFX87_07635"
     CDS             complement(17019..17240)
                     /locus_tag="VFX87_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX4994822.1"
                     /translation="MRRVPPRLVAALLLTGLVSAGLVGPGAAAALEVGQKAPDFTLPG
                     PGGKPVKLSELTPKGPVVVFTFIQAFTAT"
     gene            17370..18149
                     /locus_tag="VFX87_07640"
     CDS             17370..18149
                     /locus_tag="VFX87_07640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4994823.1"
                     /translation="MAWRIHRERVLLAGWGRAILLQIAHPKVARGVAEHSGFATDRWG
                     RLRRLHRTLSAMLALTFGSEAEAAGSAARINAIHDRVHGRLEGPGGAAYSAHDPELLT
                     WVHGTLIESFLLSYRLFVGPITTEEADRYCAEASGIEPALGIPPGRLPRTERELREYL
                     DAMLAGGSIEVTDTARRLSREIVEPPAPALVRPALRLAALPAIGLLPPAIRAAYGFPW
                     DDRRARALRVLAAASRGGLPLVPPTLRYWAVARRAERRLRR"
     gene            18146..18604
                     /locus_tag="VFX87_07645"
     CDS             18146..18604
                     /locus_tag="VFX87_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4994824.1"
                     /translation="MTEITLGIESPRQADVVRLIEALDVYQSGLYPTESNHFLDVEAL
                     AVPSVRFLVARRDGWALGCAALRIDPAGWGELKRMFVSPEARGLKLGRRLLDRIEEEA
                     RREALACIRLETGIHQPEALGLYRSAGYVEREAFGEYSPDPLSVFMEKSL"
     gene            19220..19858
                     /gene="tmk"
                     /locus_tag="VFX87_07650"
     CDS             19220..19858
                     /gene="tmk"
                     /locus_tag="VFX87_07650"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX4994825.1"
                     /translation="MPAVLITVEGVEGSGKSTQCARLAQHLGGRGLEVVRTSEPDGTP
                     LGMRVRALFEAQGPTPTPLTQTFLFMAARQEHVTRVIAPALARGAVVISDRYADATVA
                     YQGYGQGMDVQTIRELNMLATGGVVPDLTLVLDLDPVDGMRRIRGRALDTFEKMDTAF
                     HRRVREGYLEIARADKNRVVVLDADREPDALHADIVRAVDELLARRGGLRGA"
     gene            19851..20735
                     /locus_tag="VFX87_07655"
     CDS             19851..20735
                     /locus_tag="VFX87_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX4994826.1"
                     /translation="MLDAIHGQAEAVTLLRRAISSDRVAHAYAFVGPEGSGRKATARA
                     FASALVAPQGGPAAARVERGSHPDVHLLGPTPPPSNPKGTPALRIEAIREMERRAALK
                     PAEAAWKVFIVDEAEKMTASTPQALLKTLEEPPARTVIILVLSQIRALPATVLSRCQV
                     VRFRPRLVEGAPALFPDVRGAAHVRSLRLLAEAREQGVEAILRLGDQVGRDRQAAEAF
                     VEACWLWHRDLLCGLAGGPPRLAVFGEAAAKAGTLSLDQVLGALRDCREAWFALQGSV
                     SPRLSVEVLLGRLTGKAA"
     gene            20737..>21182
                     /locus_tag="VFX87_07660"
     CDS             20737..>21182
                     /locus_tag="VFX87_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HEX4994827.1"
                     /translation="MTEPLEPAATAASVAEETPQYLIGVRLREPLLAEDHLTTETDFH
                     VGDLVVVDMGGGTAVGEVRRPRRPLPSFKRDRVYRRVVRRATHAEAGEWHDRRAREVR
                     GVETCQRMARGRALAMKIVDVEIEASRRRVTVFFNSEERVDFRDLVR"
BASE COUNT         2964 a         7623 c         7642 g         2953 t
ORIGIN      
        1 tgggcccccg agcgcgccga gctcgacgag atcgcccgct tccactcgcg cgagtacatc
       61 gagatcctgc gcgcggtgag cgcgggcgac tgggtgccgc acgccgcccg ctacgggctc
      121 ggccccggcg acaacccgat cttcccgggc ctgtgggagg ccgcgcagct cggcgcgggc
      181 ggctccctgc tggcggcccg actggtcgcc gacggggagg tcacccgggc ctttcacttc
      241 gcgggcgggc tgcaccacgc catgcccggc cgcgcctccg gcttctgctc cgtcaacgac
      301 gcggtgctgg ccatcatgcg cctgcgccag ggcggcttgc gggtcgccta cgtggacatc
      361 gacgcgcacc acggcgacgg ggtgcagttc gcgttctacg acgatccgaa cgtgctcacc
      421 gtgtccaccc acgagcgggg cgaccggctc ttcccgggca ccggcttcgt ggtggagatg
      481 ggcgagggcg cgggcctcgg gtactcggtc aacgtgccgc tccagccgct caccgacgac
      541 gcggtctacc acgaggcgtt cgaggccgtg gtccccccgc tggtgaccgc cttcaagccc
      601 gacgcgctgg tcatccaact cggcatcgac tcgcaccgga ccgatccgct cacccacctg
      661 agcctcaccg tgcagggctt tacccgcgca gtgaagcggc tcctgtccct ggcgccccgg
      721 gtcgtcgcgc tgggcggcgg gggctacgac ctgaccaacg tggcgcgggc ctggacggcg
      781 gcgtgggcgg ccatgaacga cgtggacctg cccccggacc tgccccgttc gagccaccgg
      841 gacatgcagc ggctcgggct ggggaccctg tcgctgaacg acccggtcga ggtcttgccc
      901 cccgacaccc gccggtgggc cgaggagtac gcgcgccggc aggtggggga gatccaggac
      961 cggatattcc cgctgcacgg gctgtagaat cgaggggtgg acacgacgcc cggcgcctcg
     1021 gtagtggctc cgtccgcccc gctggaagca gtggcccccg acgctcccgc acgggctccg
     1081 atccggatcc cgctcaaggt caagctgagc ctcctgatca ccgcgctcgt ggtcctgacc
     1141 gtcgccctgg tcggcctctt cctcctccgc cagcagcaac agagcctgac cgcggagatg
     1201 accaagcgcg ggctcaccat ggcggagaac ttcgcggccg gggccaaaac gccgctgctc
     1261 accaacgacg agctgaccct gggcgtgctg gtgaccgacg cgatgaagga tccggacgtg
     1321 gcgtacgtga tcgtctcgga ccaggatggc aacgtgctcg cccactccga tgcccgcgcg
     1381 gtgaagaccg cggtggtgcg gcccaaggag ctcgagccgc tcaagaaccg ggtgctggtc
     1441 cagacctaca agacgccgca gggccgcatc atcgacttcg cggtgccgct ggtctacagc
     1501 ggcgtgcccg tcggcgcgct ctacctgggc ttcagcgagg aggcgatcac cgccgcgctc
     1561 gcgagcgcgc gcaaccaggc cctgctgatc accctgctca tggtcctggc cgggctcggc
     1621 ggcgcggtgg gcctggccgc cctgctctcg cgcccgatct tccgcctcgt gcaggccacc
     1681 ggagcgatcg ccgccggtaa cttcaacatc gaggtgcccg tggcctcccg cgacgagctg
     1741 ggcgcgctga ccgactcctt caaccggatg gcccgcagcc tgcgcgagaa ggagatgatc
     1801 aagcgcgcgt tcacgcgcta cgtggcccgt gaggtggtgg aggagatcct gaaggacccg
     1861 gagcacctgg tgctctccgg cgagcgccgc caggtcaccg tgctcttctg cgatgtccgc
     1921 gggttcacgc cgatgtcgga gcggctcgcc ccggaggagg tcgtgctgct gctcaacgac
     1981 ttctacaccc tgatgatcga gaccaccttc aagtacgacg gcacgctcga caagttcctg
     2041 ggcgacgcgg tgatggcggt cttcggggcc cccatggccc atcccgacca ctccgcgcgc
     2101 gccatccgca ccgcgctcgc catgcaggaa ggaattaccg gcctcaacga gcgccgggtg
     2161 cgcgacggca aggaggccat ctcggtcggc atcggggtca gcgccggcga ggcggtcgcg
     2221 ggcacggtgg gcaccgagga ccgcatggag tacaccgtca tcggcgacag cgtgaacctc
     2281 gcggcccggc tcgagtccaa tgccaagccg ggccagatcc tcatcagcta ccgcacctac
     2341 gagcgggtgc gcgacctggt ggatgcgcgc ccgctggggc gcatccgggt caagggcaag
     2401 gaagaggagg tcgaggtcta cgaggtgctc ggcctgatgc ccgtagtgtg agcgcgttct
     2461 cgtcccggca gcgtaggata tgagggtgat gaagcgacgt gcgcggggat cgggtctcct
     2521 ggtgagcgcc gccgcgctgt ggctcgccgc ctgtgccacc gcgagcgcac ccgcgcccgg
     2581 cccggcgccc gctgccccgg cgcccgctgc cacggcgccc gcccggccgc agccgcgtcc
     2641 cgccgcgtac cagtcggagg acttcatcgt caccttcgct cagcccggtg acacgcccga
     2701 gaccctcgcc gagcgctacc tgggcggcgc cgagaagggc tggatgatcg aggactacac
     2761 ggggctccgc gccttcgcgc ccggccagga ggtcgtgatc ccgcgccggc cgtggaaccc
     2821 ggccggggtg acgccgggcg gctaccagat cgtcccgatc ctcacctatc acaacctggg
     2881 cgagcaggcg aagggccggc tcgtgctcgc cgccgcgagc ttccgcgagc agatgcggta
     2941 cctcaagacc aacggctacc gcgtggtgag cctcgccgag ttcgtcgagt tcacccgcct
     3001 caatcgccag ctcccgcagc gcgcggtggt gctcaccttc gacgacggct accgcgcgtt
     3061 caaggacctc gcctatccgg tcctcaagga gcttggcttc accgccaccc tcttcatcta
     3121 tacggactgg gtgggcgcgg gccgcggcgc gctctcgtgg gccgatctac gcgagctggc
     3181 cgccgccggc atggacatcc aggcccacag caagacccac gcggacctgc gccgcgccca
     3241 gggcgagacg gaggcccagt acgcgcgccg gatgcaggcg gagctcgagc agccgcaaga
     3301 cctcttcaat cgcaacctcg gccgccgcag ccagatcctg gcctacccct acgggcgctg
     3361 ggaagaaggc ctgctgccca aggtgaagga gcacggctac atcgccgcct tctcggtgcg
     3421 ccggcagggc aatgcgtcct tcgtgcggcc cctcgccggc catcggagcc agatctactc
     3481 cgagatgacg ctcgacgact tcgtcaagaa cctgaacgtc ttccaggagg agaacctgag
     3541 gtgacgggcg cggggcgtgg atggctcgtc gcggcggcgc tcggcctggc gctgggtggc
     3601 tgcgcgtccc tgagcccctc cgccccgagc gagccggcgg cggggcccgc cccgatcgcg
     3661 cgggacccgc tcgcggcgcg gcaccgacag caggcggagg cgctcgagcg cgacggccag
     3721 ctccggcgcg cggccgagga atggaagatc gcgctcgcga tcgcgcccgg cgacgcgcgg
     3781 gcgcgcgagt ccctgcgggc cctgcaggcg cgcatcgacc aggcggtggc cgagcggctc
     3841 gacgaggggc gcaaggcgct cgcccgcggc gtgcaggtcg aggcgcgccg caagttcctg
     3901 gccgcgctcg cgctcgaccc gtccaaccgc gcggccttcg acgcgctcca gagcgacacg
     3961 cgcgaggtgg agttcctcac ccacaccgtg cgggcgggag atacgctgac cacgctggcc
     4021 cagcgctact acggcgaccg ctcgcggggc gaggtgatct gggagaccaa ccagctcccg
     4081 cccaatccgc gcctggcccc gggcaccacc ctcaagattc ccgagatccc cggcgtgccg
     4141 ttcatgcacc cggaggcgcg ccggcccgcg cccgcggtgg cggccatccc cacgccggac
     4201 gtgccacgcc cgggtccgac cccgggcccc gcggcgccgg cggcgccctc ggccaaggag
     4261 gagttcacgt ccgaggtcaa tccgctgctc gcggaggccc gcgaggcgct cgagcgcagc
     4321 gactatcccg acgcgatcgc gacggtggac aagttcctgt ccggcaagcc gggcgatcgc
     4381 gaggggctga ccctcaagaa gcaggcgctc tacggccagg ccaagagcca gatggaggcg
     4441 cgccagtacc cggaatccat ccagagcctg aaccagctcg cgcgcctgca gcccgactac
     4501 gaggacgtac ccagcctgct ccgtcaggcc cgcacccgga tcatcgagca gcactacacc
     4561 aagggcgtgc gcttctaccg cgaggagaag ctcaaggaag cgatcgcgga gtggcgcgtg
     4621 gtactcgaga tggatccggg caatgccaac gcccgtcgca acatcgacca ggcggagaag
     4681 ctgctgcgcg ggctcgagca acgcatgaag aagtagtcca accgactatc aggagaactg
     4741 gaccatgaac agacacgttc gcaccgtcgc cacgctgctg ccgatcatct cgctcaccct
     4801 gctcctcgcg ggctgtccca agcgccccgc gatgaccgcc gcgaccgccc cgccgccggt
     4861 gccgcccgcc gcggtggcgc cgcccacgcc ggctgcgccg gcccccgctc ccgcgcccat
     4921 cgccccggct ccggtggccc cgcccaccgt cgccccggct cccgcgcccg cgccgccggc
     4981 cccgccgaag gagtaccgga gcaacgcggc gctcaaccag atcttcttcg ccttcgacaa
     5041 ggcggacatc cggtccggtg acgcccagat ccttgcggcc agcgcggcct atctcaaggc
     5101 caatcccaac cagctcgtgc tgatcgaagg gcactgcgac gagcgaggga cctccgagta
     5161 caacctggcc ctgggcgagc ggcgggccaa ggcggccatg aactacctcg tgtccaacgg
     5221 ggtcgaggct tcccggatca ccacgatctc ctacggcaag gagcgcccgg gctgcaccga
     5281 gaagaacgag gcctgctggt ccaagaaccg gaacgacacg ttcttgacca aggagcgctg
     5341 aggagcggac ggatcgacga ttgactgact ctcacggccc gcggtcgtga ctgggaggcg
     5401 cccatgcccg tccaagactt gatcctggat ctcgcgatcc gctacggatt ccaggttctc
     5461 ggcgcgttcg tgatccttgg ggccggcgtc gcggtggcgt ggtgggtggg caacgcggtg
     5521 gagcgcccgc tcacccgcat ggcgctcgag ccgccgatgc gccgcctgat ggtgcgggtc
     5581 gtgagagtgg tggtgctgct gttcgcgctg gtgatcgcgc tcgacaagtt cggcttccag
     5641 atcgccccgc tcgtcgccgg catcggggtg gccggcctcg gcatcggcat cgcgctgcag
     5701 ggcgtgctgg gcaacgtcat cgcggggctc accatcatct tcaccaagcc gttccgggtg
     5761 ggcgagcacg tcgaggtggt cggcatgaag ggcgacgtgc acacgatcga gctcttttcg
     5821 accacgctgc tgcatcccga ccgctcccgc atcatcattc ccaaccgaaa gatcgtgggc
     5881 gagatcctcc acaacttcgg caatacccgg cagatccacc tggccgtgat ggtgccgcac
     5941 ggctccgacc tggccgcggt gctgaccctg gttcgcgagg tcgtcgtcag caacccccgg
     6001 gtgctcgccg atccggaccc ggtggtcggg gtggcccagc tggagctggc cggcgtccgc
     6061 gtgggggtga atccgtgggt gcgggtgccg gacgtcgtcg ccgccgaggc ggagctctac
     6121 cgggccctgg cggatcggtt cgccagatcc gggatccagg tcccacttcc gaagcaggaa
     6181 gttcgggtac tcatgagctc gtgacgggca aaatttgact cggcggcccg gcggcgccta
     6241 ggctcgagca acggagggag ccatgaagac gctgccgctg gccgtgctgt tgctgctcgc
     6301 gagcgccggg atcgcgccgg cccagacgaa gtccccgggc ccgctcgatc tggagtacaa
     6361 gcagcgggcg cccgccctcc gcccggtgcc gccgcagaag atcgaggagg acacgacgca
     6421 ggcggtacgc gagctcgatc gccagcgcac cgaccgcatg ctgcgcgccg tccagccggg
     6481 cccgtctcgg cggcctgacc tcgaccgcga cgtcacccag ggcatccaga cccgcggcct
     6541 aggccgttag cggcgcttcc gcccgcccgt ccgctcacca cgggttcgcg tctcttccag
     6601 aaccggcacg ccgtcgcggt agttgacgtg atggccgggg acggcggtaa cccgtgggcc
     6661 cggggtgatg atgccgacca ggttgagcgg gtcgcaggcc gagatgcgga cggtctggcc
     6721 ggagggctct tcgcggcgga tggcgcgcag ggcttcgagc gcttcgggcg ccgcgaactg
     6781 ctcgcccacg aagccgccca cgatacgccc gccccgcagg tcgccgcgca tctccaggcg
     6841 ccggtagacg cgcagcagat cccgccaggc gggcacgtcc ggctcgcgct gcaggagatc
     6901 gcggaagacc acgccgtagc gcctcacgta ctggcgggcc ttggcctcga cgatgtcgtc
     6961 ggtggccggg gtggtgggcg gtagcagccg ggcccaccgc ccggtgccgg cggagggccg
     7021 aggcgcgacg ggccggccgg gccgcggccg acgccgcttc tcgccgtgga gcaggctgcg
     7081 caggcccgcg aagccgtccc cggtgacgag cccggccgtc accagctcgc agagcgcttc
     7141 ctccacctcg atgcgcagcc gccgcacccc ggaggcgatg tcgtccacga aggacgcgcc
     7201 cgcgcgctcc aggtgggcga gcacatcgcg cgcgggcccc gagggctgga tcggtgacgc
     7261 ggcggcgggc gtccggtccg gggcgtcggc gcccggcgcg ggccgcggcg cgagcagcca
     7321 ggcgagatcg cgccgcagca gcagggccac cggcaccgtc gcggacaggc gccggccggc
     7381 gtggcgcgcg ctctcgtgca gcccgagccg tccccacgcg acctcgccgg aaaggcagag
     7441 ctcgtcgagc cagcccggct ggtacccggc cacgcgtgag ggcaggaggt cccgctccca
     7501 gacgccggcg gcggcctcga agccctgcag gcgctcgatc acgcgcagca gcccgtcgcg
     7561 gccgtgctgc tgggtcgcgg ggcgcgcgtg ctgccaggac agcaggaagc gcaggagcac
     7621 cgcgggcgac accggctcga tgagccggcg caggccgtcg agggtgcggc gatggatgcg
     7681 cgccagcagc cgtcgctcgc accactcgac ctgcccggcg gcgatggcct cgggcgtgaa
     7741 tcgcccgcgg agcacgccac cctcggcctc gaccgcggcc agcgccgcgt ccacctggcc
     7801 gggcgtgacg tcgagcgccg tcgccagcgc ggccgtggtg ctgggaccga ccagcccgag
     7861 ccgggcccgc acgacctcca ccagcgcgcc gtcttgaccg gaccacatgg gcgtccgccg
     7921 ggcgggcggc tcgatcaggt cgggctcgaa gcgagagtcc ggccacacgg tggcggccag
     7981 cgagcgccgc tcggccggca cccagaagac gtggctcccg cggcggagtc gggctgcgcg
     8041 cccggccgcg accagctcgt cgaggtagcc ggtccacgcc tcgccgtcgc gctcgggcag
     8101 cgcgcccagg tcgagcagca ggtcgtgcag ctcgtcggcg ctccggaggt cgggccaggc
     8161 ttcgttcacc accgcggtga tcgcggcggg gtcgagacgc ccgacatcgt cggtcacgga
     8221 cgcgggcagc ccgcggcgca gcgtgaccgc ccgcgcgcgg cgctcctcca gcggggcgtc
     8281 gtcgaggaac gcgtagggat tggcgttcag gatctcgtgc gcgaagaccg acggctcggg
     8341 tgtctcccgg gtgatccgct tgatctcccc cgcgtggagc gcggtgagcg tggcgcgcag
     8401 gccgtcgatg tccatcgcct cggtgaggca gtcgcggagg gtctcccgca cgagcgggtg
     8461 gtcgggcacc tcgatgggcc ccggctccgc gttgtcctgg caggcgagct gggcgggaaa
     8521 gaccgcgacg aggagatcct cggcccgcat gcgctgcaga tacggcggca ccttccgccc
     8581 gttctgccag cgcagcagcg cgagcgcgcg gctggcgttc cagcgccagc gggtattgaa
     8641 catcggcgcc tgcagcgcgg cctggatcag gagctcctcg atcccgctcg ggtgcagcat
     8701 ctcgaacacc gcctcgagcg ggaagctgtg ctgcgggccg agggagagca gcaccccgtc
     8761 gtcggtggcc gcggcctgca gctcgaagtt gaagctgtga cagagccgct tgcggagcgc
     8821 catgccccag gcgcggttga tgcggccgcc gaagggcgcg tggaccacga gctgcatgcc
     8881 cccggcctcg tcgaagaagc gctccaccac caccgtctcg cgagtgggca ccgcgccgag
     8941 cgcggctttc gccgcccaga tgtagtcgcg ggcgagcagg gcgccgcggc ggtcgaggcc
     9001 ggtctcccgc tcgagccagg ccgccgcgct ctccgggtcg tgcaggcggt cggccacgcc
     9061 ctggcgcacc gcggagagct ccgcggacag ctcgagcgtg cgcgcggggc cctcgccgag
     9121 ccagaacggg atgttggagg gcgccccgcc cgcgtcctcg acgcgcacgc gtccgctctc
     9181 gatccggcgg atgcgccagg agctgttgcc cagcaggatc acgtcgcccg ccatggactc
     9241 gatcgcgaag tcctcgtcga gcgagccgat ggtggtctcg tcgggctcca gcacgacctc
     9301 gtagctcgcg ttgtcgggga tcgcgccgcc cgaggtgagc gcggccagtc gcgccccgcg
     9361 ccgggccttg agccgccgcg ccactccgtc gcgatgcagc cgcgccgcgt agcggccccg
     9421 ccgcgtcgcg atgccctcgg ccagcatgtc cacgacctgg tcgaagtcct cgcggccgag
     9481 cgccgcgtac ggcgccgcgc ggcggcagag atcgaagagc ccgtcctcgt cctgctcctc
     9541 gcaggcgcag atcgcgacga tctgctgggc caggacgtcg agcggcgcgg tgcgcaggcc
     9601 gagccggtcg atctcgccgc agcgggccgc gcgtaccagc gccgcgcact ccacgagctg
     9661 gtcgcgggtg agcgggaaga gccggccctt cggcgtggcg agcagcgcgt ggcccgagcg
     9721 ccccacccgc tgcagtgcgg tggcgatcga gcggggcgag ccgatcaggc aggtgagatc
     9781 cacggtgccc acgtcgatcc cgagctcgag cgacgcggtc gccaccacca gcgagagctg
     9841 ccccgccttg agccgttgct cggcgtcgaa gcgccgctgc cggctcaggc tgctgtggtg
     9901 cgcggccacg cgctcggcgc cgaggcgctc ggccagatgg cgggtcaccc gctcgaccag
     9961 gcggcgggtg ttgacgaaca cgagcgtgga gcggtgcgcg cgggccagct ccgccacgcg
    10021 atcgtagatc tcctcccact gctcgttggt gcacaccgcg cccagctcgt cgccgagcac
    10081 ctcgatggcc aggtccatgt cgcggcgctg gcccacgttg acgatctcgg ggagccggcg
    10141 gtccgcgccg acgaggagcc gcgccgcggt ctggatcggc cgcacggtgg ccgagaggcc
    10201 gatgcgctgc aggcgcgcgc cccgcgtggc caccagctcg gtgagccgct cgagcgagag
    10261 cgccaggtgc gcgccgcgct tgtccccggc caccgcgtgg atctcgtcca cgatcacggt
    10321 gcgcacgtcg gcgagccagc gccggctcga ctcggcggtg agcaggatga agagcgattc
    10381 gggcgtggtc accatcacgt ggggcgggcg gcgcccgatc agccgtcgct cgtgcgcggg
    10441 ggtgtcgccg gtgcgcactg cggtgcggat ctcgggcgcg ggcaggcccg ccgccacggc
    10501 cgccgcggcc agctcggcga gcggctcgtc caggttgcgc cgcacgtcgt tggagagcgc
    10561 cttgagcggc gagacgtaga ggatcgcggt ccggtcggcc agccggccct cgaccgcctc
    10621 gcggaagagc cggtcgagac aggccaggaa ggcggcgagc gtcttgccgg agccggtggg
    10681 cgcggtgagc agaacgtcct ggccgtccgc gatcagcggc caggcgccgg cctggaccgg
    10741 agtgggctca ctgtagcggg cgcggaacca cgactcgacg acggggtgaa acatcgcatc
    10801 agtatagtca gattgcgtac ggcccgcacg cggggctgcg cgccgcgagg cgaacccagt
    10861 tccgtggggc gcaggagatc cttgccgaat cacgcggttc ccgtgcggcc gcgcccggcc
    10921 acctcggtgc gccatgcgtc cgttcgacga cccgaacgag gcgcacgttc ccgcctcccc
    10981 cacgcccgga gacgctgata gactcgcggc cagactgcga ggagagacca catggactcc
    11041 agctgctcga tgccgcgccg cacgttcatg gcgatgatgg ctggcggcct cctcactgca
    11101 ccgctcgccg ccgaggcgca gcaggcgagg gtgccaaaga tcggcgtgct tctcctgttc
    11161 ctgttcggca cgccggggca gcccagcccc ttgctcgacg ccctccgcgg agggctccga
    11221 gacctcgggt acgtcgaggg gcagaacttg gtcatcgagt accggtgggc agagggacag
    11281 gcccagcgtc ttgccgagct cgccaccgac ctcgtccgcc tcaacgtcgc ggtgatcgtg
    11341 accgggggta cgcccgcaac cctggcggcg aaggcagcga ccacggcgat ccccgtcgtc
    11401 atgacaaatg tgggggatcc cgtcgggagc aacatcgtgg cgagcctggc gcggccggac
    11461 gccaatgtca cggggctgag tctcctcgag acagatctgg acggcaagcg gatcgagtta
    11521 ctgaaggaag ccgtaccaca cctcacgcgg cttgccatcc tttggagcgc aaatgacccc
    11581 gggatgacac ttgcgttcgg ccgggtggga atggcagctc aggcgctggg acttgccctc
    11641 cagaacctcg cggtgcgtga gcccgccgac tttccgcgtg ccttcgacgc ggccgtcgcc
    11701 gggcgagccg aggcgctctt cgtgacggcc cagccgttca cgaatcgcca ccgggccgag
    11761 atcctggccc tcgtggccaa gcagcggctg cccgcgatgt acacgcttcg aggcttcgtg
    11821 gacgcgggcg gcctgatggc gtatggcccg agccagactg atcagtaccg ccgtgccgcc
    11881 acgtacgtgg atcggatcct caaaggcgcc aagccggcgg acttgcccgt cgagcaaccg
    11941 acgacgttcg acctggtcat caacctcaag accgccaagg ccctcggcct gacgatgccg
    12001 ccgtcgctcc tgcagcgggc ggatcaggtg atcgagtgat ggaccggcgt tcattcctcg
    12061 gcaccttggc gggcggcctc ctcgccgcgc cgctcgccgc cgaggggcaa caggcagcca
    12121 gggttgcccg gatcggtctg cttcgcccgg gtgcgcctac ggaccccgct gaccggtctt
    12181 ccgtcggcgc gttcgtggcc ggtctgcagg aggctggcta tacgcaagga acgaacatcc
    12241 gagtcgaaac ccggtatgct catggggagt tcgaccgact gccggccctc gcccgcgagt
    12301 tggccgccct tccggtagat gcgattgtca cgtcgaaccc gtacgcgacc cgagcggtcc
    12361 gagcggcgac tcgcacggtt cccatcgtcg ttgcgctcga ctacgagacc gatccggtcg
    12421 ctagcgcgtg gatagctggc attgccaggc ccggcggtaa cctcaccggc ttgttcctcg
    12481 accaacccgc gatggccggg aagctgctcc agtttctgat ggaaggtgtg ccccgtctcg
    12541 cgacggtcag cgttctgtgg gatgagacca tcggtcgaat ccagttcgac acgatggaga
    12601 aggtcgcgcg cgagaagggg ctcacgatca gatcgttcgg ggtgaagcgc tcggaggatc
    12661 tcgctgacgc gttcggcggc gctgcccgca agcgggccga cggcttggtc gtcttgacct
    12721 cgccgctgct ctccctgaac cgcgcacaga tcggggccct agcgctcagg caccgcttgc
    12781 ccgggatcac cctgttcacc aactttccag cgttcgggct cctgatggcc tacgggccga
    12841 accaacccga cgccttccgc cgtgccgcga gcgactacgt ggcgagaatc ctcaaggggg
    12901 atcggccggc cgaccttccc gtccagcgcc ctgagaagtt cgacctagtc atcaacctca
    12961 agacggccaa ggccctcggt ctgacgatgc cgccgtcgct cctgcagcgg gcggaccagg
    13021 tgatcgagtg agcgatgtgc cttcccgaca ggccgaaggt tatccggcga tgatcgagct
    13081 gtgcctgagg gccggtgccg attgactagg ccgtcacacc tccatagcct ggtcgcggta
    13141 tgacggcctc gtctgagacc gtgatcgtgg gcggcgggca cgccgggctc gcgctcagct
    13201 accatctggg ccggctcggg cgcccgcacg tgatcctcga gcgggggcgg gtggccgagc
    13261 gctggcggag cgagcgctgg gactcgctga tgttccagtt ccccaactgg agccttcgcc
    13321 tgcccggcca ggactatagc ggcgacgatc cggacggctt cgcgacgcgc gacgaggtga
    13381 tcgccttcat cgagcgctac cgcgcgctcg tcgccgcgcc ggtgcggacg ggcgtgcgcg
    13441 tggatcgcgt ccgtccggtc gacggcggct tccggctcga gaccagcgag ggggacgtcg
    13501 acgccaccaa cgtggtcgtc gccaccgggc cctatcagga gccgatcttg ccggccgtcc
    13561 gccaggcgct gccgctgggc gtcgtccaga tgcacgcgag cggctatcgc aatccggcgc
    13621 agctcccccc gggcgcggtg ctcgtggtcg gctccggcgc ttccggctgc cagatcgtgg
    13681 aggacctgct ggccgccggc cggaccgtgt acctctcggt gggccgccat cggcgctatc
    13741 cgcgccgcta ccgcggccgg gacatgttct ggtggatgga gcggatcggc gcgctcgacc
    13801 agacgctcga ggagcgcccc gacgcccgcg agcggccgaa cccgctcgtc accggagtcg
    13861 gcggcggcca cgacatcgat ctgcgcgact acgcggccgc cggggtcacg ctgctcgggc
    13921 atctccgggc cgtcacgggc tctcggctct acctggctga cgatctcgag ccgctgctcg
    13981 ccgcgggcga cgagagcgtc ggcgtgttca cgcgcgcggt ggatgcctac atcgcgcggg
    14041 ccggtctcgc ggctccggcg gagccgccgc ctccgcccgc gtcgccccgt acggcgccgg
    14101 cgccgatccg cgagctggac ctggccgctt caggcatcac gtcggtggtg tgggccaccg
    14161 gcttccggcg cgacttctcg tggatcgacg cgccggtctt cgacgagcgg ggcgagccga
    14221 tccaccggcg gggcgtcacc ggctgcgagg gtctctactt cctggggctg ccgtggctgc
    14281 acaagctcaa gtcgtcggtg ctgtgcgggg tcggggacga cgccgcccat ctcgccgacc
    14341 acatagcgta cgatctgatc ggtgcacggt cgccgcgggc gccacgcgcc cccggccggg
    14401 actgatcagg agggcgtgat ggccatcgag ttcatcggca tgatcggggt ccggcccgaa
    14461 ggggccgacg gcgccgcggt gcacgtgatc ggcggcgaga tcgacatggg atgggtccgg
    14521 gagttcgccc aggcccacga gcgggccggc ttcgacaagg tgctggtggg ctacacgtcc
    14581 acctccgcgg acggcttcct ggtctcggcg tatgccgccg cctacaccga gcgcctcggc
    14641 tatctgctcg cccaccgccc cggcttcgtc gcgcccacgc tggccgcgcg caaggcggcc
    14701 acgctcgatc agctctcggg cggccggctc gcgctccaca tcatcaccgg gggcagcgac
    14761 gccgaccagg cgcgcgacgg cgacttcgtg gaccacgaca cgcgctaccg gcgcaccgac
    14821 gagtacctcg ggctgatgcg ccgggtgtgg acggaggcgc gccccttcga ccacgccggg
    14881 gagttctacc gggtcgccgc ggcctactcg gacgtcaagc cggtgcagcg gccgcacgtg
    14941 ccgctctact tcggcggcgc ctccgaggcc gcgcaccgga tcggcgtccg gcactgcgac
    15001 gtctacatgc tgtggggcga gccgctcgcc gcgatccgcg accgcgtcgc gaccgtgcgc
    15061 gccgcggcgg cggaggccgg ccgggagccg ggcgccccgc ggttcagcgt gtcgctgcgg
    15121 ccgatcatcg cgcccaccga ggcgcaggcc tggggcaagg cgcgcacgat cctggagcag
    15181 gtgcagacga agcagggcca gcgcggcgtc ccccagcccg aggcggtggg cgcgcggcgg
    15241 ctggtggact tcgcccggca ggccgaggtg cacgacaagc ggctctggac gtcgatcgcg
    15301 gcggccgtcg gcggccaggg caacaccacc gcgctcgtgg gcacgccgga gcaggtggcg
    15361 gaggcgctgc tcgattacta cgatctcggc gtgacgacgc tgctgatccg cggcttcgat
    15421 ccgctcaacg acgcgatcga gtacggccgc gagctcatcc ctctggtgaa ggccgaggtc
    15481 gcccgccgcg agcgggatcg cgcggcggcg ctcgcgcggt gacgcggctc ctggccgtcg
    15541 tgggcgcggt gacgcctccg gggcggctcg acgcggcggt gcgcttcgcg gcggaccgtg
    15601 cgaccgaccg cgcgggcgat cggagggacg tctccgccac cgtgctgagc ctgggcgagc
    15661 atcgcgtctc cttcgcggac gggcgcccgc tcgatcgcta caccgacgat accgcgcgcg
    15721 tcgtcggcgc gatcgccgag gccgatgcgg tcctgctcgc gagcccggtc tatcgcgcct
    15781 cgttcaccgg cgcgctcaag aacctgctcg atctcacgcc ggtggaggcg ctgcgggcca
    15841 agcccgtggg catcgtggcg atgggcgcga cgctgcatca ctacctgggc gtggactggc
    15901 agctccgggc cgtgctggcc tggttcgggg cgctcgtggt gccgaccagc gtgtacctcg
    15961 agtccggcca gttccaggac ggcaagctcg cggatccggc cgcgcgcgcc gcgctcggcg
    16021 atctggtcgc cgcgctcgtc gcgatggcgg gccacgcccg ggatcaggcg ggcccgcccc
    16081 cgctggccgc cgggcgctag caggcggcag gcgacgagga ccgaccggtc agcgcctggt
    16141 ggagacgaag ccgcggatga cttcgcgcgc ggcggcaccg ctccagtcgc gggcgccgac
    16201 catccggccg accagacgcc cctcgcggtc caccaggtag gcggtgggcg gcccgaaaac
    16261 gccgtagatc cgcccggtga cgtcgcccga ggcgtcgagc agcaccggcg cggtgtagcc
    16321 gcgctcggcc acggtccgct tcacgacgtc gctactctcg cgaaagctga tcagcaggac
    16381 ctcgagcccc tgtcccttga attcctcgta gagccgattg acggaaggca actcctcccg
    16441 gcaggtcggt caccaggtgg cccagaagaa gagcagcagc gccttgcccc tgacatcctc
    16501 gagccgcacc gtacggccgt cgagcgcggg cagggcgaag tcgggcgcgg gcctgggcga
    16561 ctcgtaggcc tggacgccca gcttctccca cgccggcggc gcggcggtgg gctgcgccgc
    16621 cgcggccccg gcccagagcg ccgccgcgat caggatggac gcggcgagcg ggcgcgcggt
    16681 cacgcgcccg agtccttgag cgccttcagc acctccgcgg gatcgagcag gtcgagggca
    16741 ttgtcctgga tcttcatgta ccggaccacg ccgttccggt cgatgacgta gtaggcgcgc
    16801 ttggcatagc ggaagatcgg gctcttctcg tcggtgacca gcgcgtcgta cccgggcagc
    16861 atcgcgcggc ggaagtcgga cagcaggggg taggtgatct tgttctgctt ggtgaaggcc
    16921 tccagcgtgg ccgcgtggtc ggcgctcacg cccacgacct gcgcgccggc ggcctggaac
    16981 ttggacaggc tctcctcgaa gccgaggagc tccttggttc aggtggcggt gaacgcctgg
    17041 atgaaggtga agacgacgac ggggcccttg ggggtcagct cggacagctt gacaggcttg
    17101 ccgccagggc ccggcagggt gaagtcgggc gctttctggc cgacttccag ggcggcggcc
    17161 gcaccgggcc cgacgaggcc ggcggagacc aggccggtca gcagcagagc cgcgaccagc
    17221 cgcgggggca ctcgacgcat ggcgttctcc tctcgctggt aaggggtggc gcccacacaa
    17281 cgggcggcgg gctcccgaag tttccgggcc tcgcgcataa tgctcgcatg ggcgggtcga
    17341 cctccgctga ggctttcgtc gcgggcggca tggcttggag gatacatcgg gagcgggtgc
    17401 tcctggccgg ctggggccgg gccatcctgc tccagatcgc ccatccgaag gtggcgcgcg
    17461 gggtggccga gcacagcggg ttcgcgaccg atcgctgggg ccggctgcgg cgcctgcacc
    17521 ggacgctgtc cgccatgctc gcgctgacct tcggctcgga agcggaagcc gcgggctccg
    17581 ccgcgcggat caacgcgatc cacgaccgcg tgcatgggcg gctcgagggg ccgggaggcg
    17641 ctgcgtacag cgcccacgat ccggagctgc tcacctgggt gcacggcacg ctgatcgagt
    17701 cgttcctgct gagctaccgc ctcttcgtcg ggccgatcac gaccgaggaa gccgaccggt
    17761 attgcgcgga ggccagcggc atcgagccgg cgctcgggat cccaccgggc cggctcccgc
    17821 gaacggagcg cgagctccgc gagtacctgg acgcgatgct ggcgggagga tcgatcgagg
    17881 tgaccgacac ggcgcggcgg ctctcgcgcg agatcgtcga gccgcccgct ccggcgctcg
    17941 tccggcccgc gctccggctg gccgcgctgc cggcgatcgg cctcctgcct cccgcgatcc
    18001 gcgcggcgta tggtttcccg tgggacgatc gccgagcacg cgcgctgcgc gtcctggccg
    18061 ccgcgtcacg tggcggtctc ccgctggtgc cgccgaccct tcgctactgg gcggtggcgc
    18121 ggcgcgcgga gcgacggctg cgccgatgac cgagatcacg ctcggcatcg agtcgccgcg
    18181 tcaggcggac gtcgtgcgcc tgatcgaggc gctcgacgtc taccagagcg ggctctatcc
    18241 gaccgagagc aatcacttcc tggacgtcga ggcgctcgcg gtcccgagcg tgcgcttcct
    18301 cgtcgcgcgg cgggacgggt gggcactcgg ctgcgcggca ctccggatcg atccggcggg
    18361 atggggggag ctgaagcgga tgttcgtgtc cccggaagcc cgggggctga agctgggccg
    18421 ccgccttctc gatcggatcg aagaggaggc gcgccgagag gcgctcgcct gcatacgcct
    18481 cgagaccggc attcaccagc cggaggcgct cgggctctac cgctccgcgg gctacgtgga
    18541 gcgggaggcc ttcggcgagt acagcccgga cccgctgagc gtcttcatgg agaagagcct
    18601 ctgacggctc caccacatgc cgcccagtac cgcggcagtg ccgcccacgc tgagggcacc
    18661 cagcatcacg ccccggcgca tcgcctccca gccgcggttc atgcggacca tcacgtgggc
    18721 cgggatggcg tcgatgtcgg cgcggattcg ccagcgcgcg aaggcgagtg gatctgcggg
    18781 atgccagaag ctgccccggt cgtccgcgac acagaggtga atctcgcagc tctgcgggtc
    18841 ggcgcaccgc tcggctcgcc agccgagtcc gcgcccacgc atccccgtat tttgtagcgc
    18901 caagttatcc attgcgtcgc ccctcccgac ctgaagcttg gacccgacgg cggaggcggg
    18961 ctggatcctc gctcaggacc ggtgtgtggc gaggtctccg cactctttta aacccgcgct
    19021 ccgcctcggg tgttccagag aatagcaaga gccgcgccac caagcattcc gagggaaagg
    19081 cgggttcccc ggcgcttctc tccggacttc gctgctgcgc gttaagaagg gtggggtacg
    19141 aactgtggaa ggaattaccc tgaggtagcc cctgggtggc gatcggcgcc ccaccaaatc
    19201 agtgtgttag agtacgcgca tgcctgcggt gctgatcacg gtcgagggcg tcgagggatc
    19261 cggcaaatct acacagtgcg cccggctcgc gcagcatctg ggtgggcgag gcctcgaagt
    19321 ggtgcggacc agtgagcccg acgggacccc gctgggcatg cgggtgcggg ctctgttcga
    19381 agcccagggt cccacgccga cgccgctcac ccagaccttc ctcttcatgg cggcacgtca
    19441 ggagcacgtc acgcgcgtca tcgcgccggc gctcgcgcgg ggcgcggtgg tgatctccga
    19501 ccgctatgcg gacgcgacgg tcgcctacca gggctacggc caggggatgg acgtgcagac
    19561 catccgggag ctgaacatgc tcgcgacggg cggagtcgtg cccgatctca ccctggtgct
    19621 ggatctcgat cccgtcgacg gcatgcggcg tatccgcggg cgcgcgctcg acaccttcga
    19681 gaagatggat acggccttcc accgccgcgt gcgtgagggc tatctcgaga tcgcgcgcgc
    19741 cgacaagaat cgcgtggtcg tgctggacgc cgaccgcgag ccggacgccc tgcacgccga
    19801 catcgtccga gcggtggacg agctactggc gcggcgcgga ggcctccgtg gtgcttgacg
    19861 cgatccacgg ccaggccgag gcggtgacgc tgttgcgccg cgcgatctcc tcggatcggg
    19921 tggcccacgc ctacgccttc gtcggccccg agggctcggg ccgcaaggcc accgcgcggg
    19981 ccttcgcgtc cgccctggtc gcgccgcagg gcgggcccgc cgccgcgcgc gtcgagcgcg
    20041 gtagccatcc cgacgtgcac ctgctggggc cgacgccgcc cccgagcaat cccaagggca
    20101 cgccggccct gcgcatcgag gcgatccggg agatggagcg ccgggccgcg ctcaagcctg
    20161 ccgaggctgc ctggaaggtc ttcatcgtgg acgaggcgga gaagatgacg gcctcgacgc
    20221 cgcaggcgct gctcaagacg ctcgaggagc cgcccgcgcg gaccgtgatc atcctggtcc
    20281 tctcccagat ccgcgcgctg cccgccaccg tgctctcccg ctgccaggtg gtgcgcttcc
    20341 gcccccggct cgtggagggc gcgccggcgc tcttccccga tgtccgcggc gcggcgcatg
    20401 tccgcagcct gcggctgctg gccgaggcgc gcgagcaggg cgtcgaggcc atcctgcgcc
    20461 tgggcgatca ggtcggccgc gaccggcagg cggcggaggc cttcgtcgag gcgtgctggc
    20521 tctggcatcg cgatctgctc tgcgggctgg ccggcggccc cccgcggttg gccgtcttcg
    20581 gcgaagccgc cgccaaggcc ggcacgctct cgctcgatca ggtgctgggc gcgctgcgcg
    20641 attgccgcga ggcgtggttc gcgctgcagg ggagcgtctc gccccggctc agcgtggaag
    20701 tgctgctggg ccgactcacc ggaaaggccg cctgacatga ccgagccgct cgagcccgcc
    20761 gcgaccgccg cctcggtggc cgaggagacg ccgcagtacc tgatcggggt gcggctgcgc
    20821 gagccgctcc tggccgagga ccacctcacc accgagaccg atttccacgt cggggacctc
    20881 gtggtggtgg acatgggtgg gggcaccgcg gtgggtgagg tgcggcgccc ccggcgcccg
    20941 ctgccctcgt tcaagcgcga ccgcgtctac cgccgggtcg tccggcgcgc cacccacgcg
    21001 gaagcgggcg agtggcacga tcgccgggcg cgcgaggtgc gcggcgtgga gacgtgccag
    21061 cggatggcgc gcgggcgcgc gctcgccatg aagatcgtgg acgtcgagat cgaggcctcg
    21121 cgccgccgcg tcaccgtctt cttcaactcc gaggagcggg tggatttccg cgacctcgtg
    21181 cg
//
LOCUS       DASUFA010000208         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_51810, whole genome shotgun sequence.
ACCESSION   DASUFA010000208 DASUFA010000000
VERSION     DASUFA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51810"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFX87_07665"
     CDS             complement(<1..560)
                     /locus_tag="VFX87_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994828.1"
                     /translation="MSASRAGVLGLVAALVLGAGLAGAQAPSGPAAPAVTGPGAAAPP
                     ANLAASADAGATVARPSLPPPDLVPALRLVAPPLDKPAVPLPAIELPASPQPFPTLAP
                     PAVVSDLALRPSAPMSPPRALACNPLGSVFGVASEQLECGRAKYQRAELEAALVEFQA
                     VMQRSGDRELAREARYWTAESLLRLGR"
     gene            complement(557..1864)
                     /locus_tag="VFX87_07670"
     CDS             complement(557..1864)
                     /locus_tag="VFX87_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994829.1"
                     /translation="MGERAFEDKRHAVSRASLERLIQRFPADARIPEATLLLGKVRLA
                     QGGYEPALAAFRQAQTLTPPPGRPEEPRFWEAETLFRMGRYDAARAVYDALIAANAAS
                     PFAPDALYGLAWSQLELKRREGAVAAFRQLLEAFPEHVTAPSATYHLARALVEGKRHD
                     EAIGLLRPFPEKYRDSKLVPDARYLLGVASLGAGHASEGIAELRAFISAYPGHGLANQ
                     ARRTLVDRLVKEGRKDDLAQEYKTLMAQSPPTAEGIYDAGFIATRLGRPRDAEAAWSS
                     LRKDFPDHPLAARASYDLAQGAFGRGSFKDAAALARGAAKSPDEAVRAQAQLLVGESE
                     LKLKRPAPAHPAFQAAVEGAGSRDPAVRFRALAGSGLALEEQGKLADSLRYYDQVAAD
                     APDKELRAWAKARRTAVAAQLNPVATKPAPKPAKPGETKSGKP"
     gene            complement(2024..3433)
                     /locus_tag="VFX87_07675"
     CDS             complement(2024..3433)
                     /locus_tag="VFX87_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX4994830.1"
                     /translation="MSRKLAALALVYLFATAQVAAAASVPITRETQKDVMVTIYNGNL
                     GLVKDTREIRLPTGTNEVQFMEVAAQIDPTTVHLRSLTDPAGLRILEQNYEYDLISSQ
                     KLLEKYVGRKVRLYGNDGTYHEATLLSTNGPVYDINGQIHLGHYGRVVLPALPDNLVS
                     QPTLKWLLRNQTDRTQRVEASYLTGGITWRADYVMVINASDSKTDLTGWVTVDNKSGA
                     TFGNAALKLVAGDVNRARDVRREGRMLDIAAKAASPAAASQEFIAEGLFEYHLYTLDG
                     RTTIKENQTKQLTLMSAADVPVKKELIYYGAQEYYRNAYGMPVSNQKVAAYLEIRNTK
                     ENRLGLPLPKGKVRVYKADSAGSQQFIGEDWIDHTAKDERVKIKLGNAFDVVAERTQK
                     DWRKIGGNLYEVEWEISLRNHKTEAQTVTVIEPVPGDWQMLASTHAWEKVEAHTLKYE
                     IAVPKEGAVKLTYRVRLKF"
     gene            complement(3509..>3858)
                     /locus_tag="VFX87_07680"
     CDS             complement(3509..>3858)
                     /locus_tag="VFX87_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994831.1"
                     /translation="PRETEGPRADSKSGEPSARARQLPTRLFVAPDAAGRLTVSDRAA
                     ADRALADLGGRLGVAPAFRREEPDGATIEWILPRELYGEFVRGLAGIGRWTAEREPAT
                     LPPRVRLQVRVGS"
BASE COUNT          505 a         1347 c         1403 g          603 t
ORIGIN      
        1 cgcccgaggc ggagaaggct ctcggccgtc cagtagcgcg cctcgcgggc cagctcgcga
       61 tcgcccgacc tctgcatcac cgcctggaac tccaccagcg ccgcctcgag ctcggcgcgc
      121 tggtacttgg cgcgcccgca ctcgagctgc tcggacgcca ccccgaacac gctgccgagg
      181 ggattgcacg ccagcgcgcg cgggggcgac atcggcgcgg aggggcgcag cgcgagatcg
      241 ctcaccaccg ccggtggcgc cagcgtcggg aagggctggg gcgaggccgg cagctcgatg
      301 gcgggcagcg gcaccgccgg cttgtccagg ggcggcgcca ccagccgcaa tgccgggacg
      361 agatcaggcg gcggcagcga gggacgcgcg acggtggcgc cggcatccgc cgatgctgcg
      421 aggttcgcgg ggggcgccgc cgcgccgggc cccgtcacgg caggcgcggc ggggccggac
      481 ggagcctgag cccccgcgag gcccgctccc agcacgagcg cggcgacgag tcccagcaca
      541 cccgcgcgcg aggcgctcac ggcttgccgc tcttggtctc gccgggcttg gccggctttg
      601 gcgcgggctt ggtggccacc gggttgagct gggcggctac cgcggtccgc ctcgccttgg
      661 cccaggcgcg cagctccttg tcgggggcat cggcggcgac ctggtcgtag tagcggagcg
      721 aatcggccag ctttccctgc tcctccagcg cgagcccgct cccggccagc gcgcggaagc
      781 gtacggcggg atcccggctg cccgccccct ccaccgccgc ctggaaggcc gggtgcgccg
      841 gggccggccg cttgagcttc agctcgctct cgccgacgag gagctgggcc tgggcacgca
      901 ctgcctcatc ggggctcttc gccgcgcctc gcgccagcgc ggccgcatcc ttgaagctcc
      961 cccgcccgaa ggcgccctgc gcgagatcgt acgaggcccg ggcggcgagc gggtggtcgg
     1021 gaaagtcttt gcggaggctg gaccaggcgg cctccgcgtc gcgcggccgg cccaggcgcg
     1081 tcgcgatgaa cccggcgtcg tatatgccct cggcggtggg cggtgactgg gccatcagcg
     1141 tcttgtattc ctgggcgaga tcgtccttgc gtccctcctt caccaggcgg tccaccaggg
     1201 tgcgccgcgc ctggttggcg agcccgtgac cgggatacgc gctgatgaag gcgcggagct
     1261 cggcgatgcc ctcgctcgca tgccccgcgc ccaggctcgc cacgcccagg agatagcgcg
     1321 cgtcgggcac cagcttggag tcgcggtatt tctcggggaa gggccgcagc agcccgatcg
     1381 cctcgtcgtg ccgcttgccc tccacgagag cccgcgcgag gtgataggtc gccgacggcg
     1441 cggtgacgtg ctcggggaac gcctcgagga gctggcgaaa cgcggcgacg gcgccttcgc
     1501 ggcgcttgag ctccagctgg ctccaggcaa gcccatagag cgcgtccggc gcgaacggcg
     1561 aggcggcatt ggcggcgatc agcgcgtcgt agactgcgcg ggcggcgtcg tagcggccca
     1621 tgcggaacag ggtctccgcc tcccagaatc ggggttcctc gggcctgccg ggcggcggcg
     1681 tcagcgtctg ggcctgacgg aaggcggcca gcgcgggctc atagccgccc tgggccaggc
     1741 gcaccttgcc gagcagcagg gtggcctccg gaatcctcgc gtcggcgggg aaacgctgga
     1801 tgaggcgctc gagcgaggcc cggctgacgg cgtgccgctt gtcctcgaaa gcccgctcac
     1861 ccaccaacca tcgacggccg gcttcgtcga gggccgcggc cggccccggc gctccggcga
     1921 ggagcgccgt ccaggccgcg cacgcgagga ggcgagcgag aaggcctgac cgcgatcgcg
     1981 tcatgccctt tcaccgtacc acgggccccc cggggcttca tcctcagaac ttcagccgaa
     2041 cgcgataggt gagcttgacc gcgccttcct tggggaccgc gatctcgtac ttgagcgtgt
     2101 gggcctccac cttctcccag gcatgggtgg aggcgagcat ctgccagtcg ccgggcacgg
     2161 gctcgatcac ggtgacggtc tgagcctcgg tcttgtggtt gcgcaaggag atctcccact
     2221 ccacctcgta gaggttcccg ccgatcttcc gccagtcctt ctgggtgcgc tcggccacca
     2281 cgtcgaaggc attgccgagc ttgatcttca cccgctcgtc cttcgcggtg tggtcgatcc
     2341 agtcctcgcc gatgaactgc tggctgccgg cgctgtccgc cttgtagacg cgcaccttgc
     2401 ccttgggcag cggcagcccg agccggttct ccttggtgtt ccggatctcg aggtaggccg
     2461 cgaccttctg gttcgacacc ggcatgccgt aggcgttccg gtagtactcc tgcgcgccgt
     2521 agtagatgag ctccttcttc accggcacgt cggcggcgga catcagggtg agctgcttgg
     2581 tctggttctc cttgatggtc gtccggccgt cgagcgtata gaggtgatac tcgaagagcc
     2641 cctcggcgat gaactcctgg ctcgccgccg cgggggacgc ggccttcgcc gcgatatcca
     2701 gcatccggcc ctcccggcgc acgtcgcggg cgcggttgac gtcgcccgcc accagcttga
     2761 gcgcggcatt gccgaaggtc gccccgctct tgttgtccac cgtgacccag ccggtgaggt
     2821 cggtcttgct gtcgctcgcg ttgatgacca tcacgtagtc cgcgcgccag gtgatgcccc
     2881 cggtgagata ggacgcctcc acgcgctgcg tccgatcggt ctggttgcgc agcagccatt
     2941 tcagcgtggg ctgggacacg agattgtcgg ggagggccgg cagcaccacg cggccgtagt
     3001 ggccgaggtg gatctggcca ttgatgtcgt agacggggcc attggtcgag agcagggtgg
     3061 cctcgtggta ggtgccgtcg ttgccgtaga ggcgcacctt gcggcccacg tacttctcga
     3121 ggagcttctg gctcgagatg aggtcgtact cgtagttctg ctcgaggatg cgaagaccgg
     3181 cgggatcggt gagggagcgg aggtgcaccg tggtgggatc gatctgcgcc gccacttcca
     3241 tgaactgcac ctcgttggtg ccggtgggca gccggatctc gcgcgtatct ttcacgaggc
     3301 cgaggttgcc gttgtagatc gtcaccatca cgtccttctg cgtctcgcgc gtgatgggga
     3361 cggaggccgc cgccgcgacc tgcgcggtgg cgaagagata gaccagggcg agcgctgcca
     3421 gcttgcgtga catggattcg tcctccggtt cgactgagct cgtgtgcgtc tgcccagtta
     3481 gaccgggcgg gccggcgaga gttcccgctc agctcccgac gcgaacctgg aggcggacgc
     3541 gggggggcag ggtcgccggc tcacgctccg ccgtccagcg accgatcccg gcgagcccgc
     3601 ggacgaattc cccatacagc tcgcgcggaa ggatccactc gatcgtcgcg ccgtccggct
     3661 cctcgcgacg gaacgccggg gcgacgccca gacggccgcc cagatcggcg agcgcgcgat
     3721 cggccgcagc gcgatcgctc accgtcaggc ggcccgcggc gtcgggcgcc acgaagagcc
     3781 gggtcggtaa ctgccgcgcg cgggccgacg gctctcccga cttcgagtcg gcccgcgggc
     3841 cctcggtttc gcgggggg
//
LOCUS       DASUFA010000209         7164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1289796, whole genome shotgun sequence.
ACCESSION   DASUFA010000209 DASUFA010000000
VERSION     DASUFA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289796"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VFX87_07685"
     CDS             complement(<1..1086)
                     /locus_tag="VFX87_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4994832.1"
                     /translation="MSDLEHELRRRVEGDVRFDRYSRLLYSTDASMYQMEPMGVVIPR
                     HADDVQAAIEVANQLGAPLLPRGGGTSLTGQTVNHAVVLDFSRHMAGVLEVNREERWA
                     RVQPGLVQDELNHHVRPLGLLFGPDTSTSNRATIGGMLGNNSGGSHSIAYGLTVEHVI
                     EMTTILADGSRAVFGEVTPEQFAAKMRLPGLEGQIYREIDRIRRQYDAEIRTRYPRHW
                     RRVCGYNLDELVKDRPLNMARLIVGSEGTFLTVVEAKMRLVKKPPATAVDVIHYHDMQ
                     EALESSQAILETGPYAVELTDKMILDLARNNIEQATRMGFVQGDPAAILIVEYAGETE
                     QEVKAKVADLEARRTRGRFGYASHIATD"
     gene            complement(1148..1723)
                     /locus_tag="VFX87_07690"
     CDS             complement(1148..1723)
                     /locus_tag="VFX87_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4994833.1"
                     /translation="MARKRPLKRYLTTGKVGEILEVSPAAIKKWIQQGKLAAFRTPGG
                     HFRILADEFERFQKTHGFGTTGEPLRVLVVDDQRDVAEVIVASLRAFHPSARMETAAN
                     GFEGLLKVGTFRPDVLLLDLGMPGMDGFEVCRQIKRDPVIRDTKVVVMTARFLDAEPR
                     AMEAGADGFLSKPFEAATVYGLLARLLAARR"
     gene            1921..2349
                     /locus_tag="VFX87_07695"
     CDS             1921..2349
                     /locus_tag="VFX87_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4994834.1"
                     /translation="MPEPSEEAQQTILVVDDEPEVRALVREMLTLHGYNVIDTGDPVE
                     ARRIAEAQPIHLLLTDVVMPIMNGLELAKRVEASSPTTKIVLMSGYSTAAVKGSGRPL
                     ISKPFRTSDLVTTIRQMLDSKSAFRRPDPPPAPKPGFGPI"
     gene            2349..2873
                     /locus_tag="VFX87_07700"
     CDS             2349..2873
                     /locus_tag="VFX87_07700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4994835.1"
                     /translation="MRLAIALGALLLAAAPAAAAPAAPAFHVTLLDGSGHFDSRAHLG
                     KHVLVVRFQASWCKVCNEEAAAFERVYQKYRASGVEMVSVQVQDTEADARHFLAVNKA
                     TYPAGLDPRLQIAGRFGFRGTPYTVVIDKRGELVARIHGRSDEARLARVLDPLVKRAP
                     GPKAPAPKAPARSR"
     gene            complement(2885..3409)
                     /locus_tag="VFX87_07705"
     CDS             complement(2885..3409)
                     /locus_tag="VFX87_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994836.1"
                     /translation="MDPTGSPFRRPPRRPAFRPPRRSGFGPVPAASLTVDARSAPAAR
                     ERGRGVAREARARTLRLGAGAFYLVAALTLLDAALSFASHDPGVIIGLGLTRWVAGAT
                     PVAWAALPFVVGFLLLGLIAHRGRLWAFAIGAPVYALDGLIVVAGHDWVGVAIHTVVL
                     VMLVRGLDARRRLS"
     gene            complement(3511..4560)
                     /locus_tag="VFX87_07710"
     CDS             complement(3511..4560)
                     /locus_tag="VFX87_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4994837.1"
                     /translation="MKAITLHGPGDVRVESVPDPRLVDPTDAVLRITTSAVCGSDLHQ
                     YHGRVAGLDPGIVMGHEFMGVVEEVGPAVRLIKKGDRVLAPFSISCGFCEWCRMRLPT
                     QCATTGRAVFGGRFGKQYPGGQAEAIRVPFADHMCEPVPAGMTDEQVFFLGDILSTGY
                     VCAENAGIRPGDTVAVFGAGPVGLLALQSAHLFGPSRVFVVDRVDYRLKLAEELGAVP
                     VNLDRGDPAEQLRAQTRGRGPDAVLECVGHETPFTQAIQAVRAGGTVSSVGVYVESSM
                     GFPAREAFFKDLTLRMGICNARNFITPLMPLIQNGRLQPTRIITHTMPLSDTPKGYAI
                     FDRKEDRAIKVMLKP"
     gene            complement(4557..5471)
                     /locus_tag="VFX87_07715"
     CDS             complement(4557..5471)
                     /locus_tag="VFX87_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX4994838.1"
                     /translation="MGGTTVAAGLVAADGQVLEHRQAPTHARGPGTALDTIGGLLEQL
                     CEQARARGVTVTGVGLGVPGIVDAEHGRVGVDVHYAPELARVPLAALLGDRLGVPVYV
                     DNDVNALALAEWTWGAGRGARSLVMLALGTGVGGGIILDGRLHRGQGGFGGELGHVPI
                     DFDGPACICGGRGCLKAYVSGTDIALRGAERLGRPVGAAEVFRLAAAGHAAADAIVEE
                     VCGALGAGLAIIVNGLNPERVLLAGSVAKSLRPLEARLRAALARYAFAEALRTTHLEI
                     LTLDKDATVRGGAALVHYETQRRRTTTS"
     gene            complement(5557..6315)
                     /locus_tag="VFX87_07720"
     CDS             complement(5557..6315)
                     /locus_tag="VFX87_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX4994839.1"
                     /translation="MKPTRRLIVNADDYGLTAGVSRGILDSHRRGIVTSTTLLVNRPV
                     DPALIDALKASEMGVGLHMNLTLGPPVAPAKRVASLLDAEGKFTRDAREAAQRARPDE
                     ARIELGMQIDEFRKIMGRFPTHLDSHHHVGRHEPILDLVLDFARAIKVPVRTQDAEVR
                     AAARKLALKTPDHFMGESGPDAYWSSERVLAHLRQLPGGVTEFMTHPGYFDDDLAYSR
                     YGRQRDIELAGLTDPEARALIEREGIRLIHFGDL"
     gene            complement(6312..7040)
                     /locus_tag="VFX87_07725"
     CDS             complement(6312..7040)
                     /locus_tag="VFX87_07725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HEX4994840.1"
                     /translation="MTDSGALLFVHGGCTFTVADGVQPPKAGSLLFCRHLPVRAGDRM
                     LEIGTGIGLAAVLAARAGARVVATDVVDAAVRCARANAMINGVADRVEVLAGDGFAPV
                     RGRVFDLICTSPPQMPTPPNRERADAAAAADNGGPDGWALLDRVIAGAPAHLAPGGRL
                     VFTLFAFLGVKAALARLHDAGFEPAILGQETQAFPRIGYERIEHIRALDAEATLPRAG
                     WPATVERYVFQGTWQGTRMEDPSR"
BASE COUNT         1050 a         2613 c         2520 g          981 t
ORIGIN      
        1 gtcggtggcg atgtgcgagg cgtagccgaa gcgaccccgc gtccgccgcg cctcgaggtc
       61 cgcgaccttg gccttgacct cctgctcggt ctcccccgcg tactccacga tcaggatggc
      121 ggcggggtcg ccctgcacga atcccatgcg cgtggcctgc tcgatgttgt tgcgggcaag
      181 atcgaggatc atcttgtcgg tgagctcgac cgcgtacggg cccgtctcga ggatggcctg
      241 ggaggactcg agcgcctcct gcatgtcgtg gtagtggatc acgtcgaccg cggtggccgg
      301 gggcttcttc accaggcgca tcttcgcctc gaccacggtg aggaaggtgc cctccgagcc
      361 cacgatgagc cgcgccatgt tgagcggccg gtccttcacc agctcgtcca ggttgtagcc
      421 gcacacccgc cgccagtgcc gcggataccg cgtccggatc tccgcgtcgt actgccggcg
      481 gatccggtcg atctcccggt agatctgtcc ctccaggccg ggcaggcgca tcttggccgc
      541 gaactgctcg ggcgtcacct ccccgaacac ggcgcggctg ccgtcggcca ggatcgtggt
      601 catctcgatc acgtgctcga cggtgaggcc gtaggcgatg gagtgcgacc cgcccgagtt
      661 gttgccgagc atgccgccga tggtggcgcg gttcgaggtg gaggtgtcgg gcccgaacag
      721 cagccccagg ggccgcacgt ggtggttcag ctcgtcctgc acgaggccgg gctgcacccg
      781 cgcccaccgc tcctcccggt tgacctcgag cacgcccgcc atgtggcgcg agaagtcgag
      841 caccaccgcg tgattgacgg tctggccggt cagcgaggtg ccgccgccgc ggggcagcag
      901 cggcgccccg agctggttcg cgacctcgat cgccgcctgc acgtcgtcgg cgtggcgcgg
      961 gatcaccacg cccatcggct ccatctggta catggaggcg tccgtcgagt aaagcaggcg
     1021 ggagtagcga tcgaagcgca cgtcgccttc gacacgccgg cgcagctcgt gctcgaggtc
     1081 gctcatggcg gggattatgg cacgcgcccg agcgggcccg caattgaccg gcgcccgacc
     1141 ggcgcgctca gcgccgcgcg gcgagcaggc gggccaggag cccgtagacg gtggcggcct
     1201 cgaacggctt ggacaggaag ccgtcggcgc ccgcttccat ggcccgcggc tccgcgtcga
     1261 ggaagcgggc ggtcatcacc acgaccttgg tgtcgcggat gaccggatcg cgcttgatct
     1321 ggcgacagac ctcgaagccg tccatgcccg gcatgccgag gtcgagcagc aggacgtcgg
     1381 ggcggaacgt gccgaccttg aggagaccct cgaagccgtt cgccgcggtc tccatccggg
     1441 cgctgggatg gaaggcgcga agcgatgcca cgatcacctc ggccacgtcg cgctggtcgt
     1501 ccacgacgag cacgcggagc ggctcgccgg tcgtgccgaa gccatgggtc ttctggaagc
     1561 gctcgaactc gtcggcgagg atgcggaagt gcccgcccgg ggtccggaac gcggccagct
     1621 tgccctgctg gatccacttc ttgatcgcgg ccggagaaac ctcaagtatt tcacctactt
     1681 tcccggtcgt caggtacctc ttgagcggac gcttcctggc cacggggcgc gatccggcgg
     1741 ttgaccgcat gccaaaactc tagcagccga gccgcgaatc gaccgccgag attcgacgac
     1801 ctcggcgagg cgccggacga ccgggcccgg cggaaaaggt tacgcgatcg acgccgagcg
     1861 aaaggtcggc ctcgcacaca aaaacggtag gttacaatcg actcgcgcct ggagagcact
     1921 gtgcccgagc ccagcgagga agcgcaacag acgattctcg tcgtggacga cgagcccgag
     1981 gtgcgtgccc tcgtgcgcga gatgctcacc ctgcacggct acaacgtgat cgacaccggc
     2041 gatcccgtcg aggcccgccg catcgccgag gcgcaaccga ttcatctgct gctcaccgac
     2101 gtggtgatgc cgatcatgaa cggcctcgag ctcgccaagc gcgtcgaggc cagcagcccg
     2161 accacgaaga tcgtgctcat gtccggctac tccaccgccg cggtcaaggg ctcgggacgg
     2221 ccgctgatat cgaagccgtt caggacgagc gacctcgtga ccacgatccg gcagatgctc
     2281 gactcgaagt ccgcgttccg ccggcccgac cctccgcccg cgcccaagcc gggcttcgga
     2341 ccgatctgat gcgcctggcc atcgcgctgg gcgcgctcct gctggccgcc gcgcccgccg
     2401 cggcggcgcc cgcggcgccc gcgttccacg tcacgctgct cgacggctcc ggccacttcg
     2461 actcccgcgc gcatctgggc aagcacgtcc tggtcgtgcg cttccaggcc tcgtggtgca
     2521 aggtgtgcaa cgaggaggcg gcggcgttcg agcgggtcta ccagaagtac cgggcgagcg
     2581 gggtcgagat ggtgagcgtg caggtccagg atacggaagc cgacgcgcga cacttcctcg
     2641 cggtcaacaa ggcgacctac ccggccgggc tcgatccgcg cctgcagatc gccggccgct
     2701 tcggcttccg gggcacgccc tacacggtgg tcatcgacaa gcgcggcgag ctcgtggccc
     2761 ggatccacgg ccggagcgac gaggcgcgcc tcgcgcgcgt gctcgatccc ctcgtcaagc
     2821 gggcacccgg ccccaaggcc cccgctccca aggcccccgc ccgctccagg taagcccgcg
     2881 tcggctagga gagccgccgc ctcgcgtcca ggccccgcac cagcatcacg agcaccaccg
     2941 tgtgaatcgc cacacccacc cagtcgtggc ccgcgacgac gatcagcccg tcgagcgcgt
     3001 acaccggcgc gccgatcgcg aaggcccaga gccgtccccg gtgggcgatg aggccgagga
     3061 gcaggaatcc cacgacgaac ggcagggccg cccaggcgac cggggtggcg ccggcgaccc
     3121 accgggtcag cccgaggccg atgatcacgc ccgggtcgtg cgaggcgaag gagagcgcgg
     3181 cgtcgagcag cgtgagcgcg gcgacgaggt agaaggcacc cgcgcccagg cggagggtgc
     3241 gggcacgtgc ctcgcgcgcg accccccggc cccgctcgcg cgcggccggg gcgctccgcg
     3301 cgtcaacggt cagggaggcg gcgggaaccg gcccgaaccc gctccggcgt ggcggccgga
     3361 aggccggccg ccggggcgga cggcgaaagg gacttccggt cggatccatg ttcgctcctc
     3421 cgtcacatcg gtgtcagacg tgtccgtatc ggcaccgaag tggcccctgg acgaggccat
     3481 gatcctagcg gagcaagcgg tgtgccaggc tcagggcttg agcatcacct tgatggcgcg
     3541 gtcctccttc cggtcgaaga tcgcgtagcc cttcggcgtg tcggagagcg gcatcgtgtg
     3601 cgtgatgatc cgcgtgggct gcaggcgccc gttctggatc agcggcatga gcggcgtgat
     3661 gaagttgcgc gcgttgcaga tgcccatgcg cagggtgagg tccttgaaga acgcctcccg
     3721 cgccgggaac cccatggacg actccacgta gacgcccacc gaggagacgg tgccgcccgc
     3781 gcgcaccgcc tggatggcct gggtgaacgg ggtctcgtgg cccacgcact ccagcaccgc
     3841 gtccgggccc cggccgcgcg tctgggcgcg gagctgctcg gcgggatcac cgcgatcgag
     3901 gttcaccggg acggcgccca gctcctccgc gagcttcaga cggtagtcca cgcggtccac
     3961 cacgaagacg cggctcggcc cgaagagatg cgccgactgc agcgcgagca ggccgaccgg
     4021 gcccgcgccg aacaccgcca ctgtatcgcc ggggcggatc cccgcgttct ccgcgcagac
     4081 gtagccggtg gagaggatgt cgccgaggaa gaacacctgc tcgtcggtca tcccggcggg
     4141 gacgggctcg cacatgtgat cggcgaacgg cacccggatg gcctcggcct gcccgcccgg
     4201 gtactgcttg ccgaagcggc cgccaaacac cgcgcgaccg gtggtggcgc actgagtggg
     4261 cagccgcatg cggcaccact cgcagaagcc gcacgagatc gagaagggcg cgagcacgcg
     4321 gtcgcccttc ttgatcagcc gcaccgcggg gccgacctcc tccaccacgc ccatgaactc
     4381 gtggcccatc acgatgcccg gatcgagtcc cgcgacccgc ccgtgatact ggtgcagatc
     4441 ggagccgcac accgcgctgg tcgtgatgcg cagcaccgcg tcggtggggt ccacgagacg
     4501 cggatccggc accgattcca cccgcacgtc tcccggtccg tgaagcgtga tggctttcat
     4561 gacgtcgtcg tcctccgtcg ctgggtttcg tagtggacga gcgcggcgcc gccccgcacc
     4621 gtcgcgtcct tgtcgagggt gaggatctcc agatgcgtcg tgcggagcgc ctccgcgaag
     4681 gcgtagcgcg ccagggccgc acgcaggcgc gcctcgagag gccggagcga cttggccacg
     4741 ctgcccgcca gcagcacccg ctccggattg agcccgttca cgatgatggc cagccccgcc
     4801 ccgagggctc cgcagacctc ctccacgatg gcgtccgccg ccgcgtggcc ggccgccgcg
     4861 agccggaaga cctcggccgc gccgaccggg cggccgagcc gctcggcgcc acgcagcgcg
     4921 atgtcggtgc ccgacacgta ggccttgagg cacccgcgcc cgccgcagat gcaggccggc
     4981 ccgtcgaagt cgatcggcac gtggccgagc tcgccgccaa agcctccctg cccgcggtgg
     5041 agccggccgt cgaggatgat gccgccgccg acgccggtgc ccagggccag catgacaagc
     5101 gaccgcgccc cgcgccccgc gccccaggtc cactcggcca gcgcgagcgc gttgacgtcg
     5161 ttgtccacgt agacgggcac gccgaggcgg tcgcccagca gggcggcgag cggcacccgg
     5221 gccagctcgg gggcgtagtg gacgtccacg ccgaccctgc cgtgctcggc gtccacgatc
     5281 ccgggcacgc cgaggccgac accggtgacc gtcacgcccc tggcccgcgc ctgctcgcag
     5341 agctgctcga gcagcccgcc gatggtgtcg agcgcggtgc ccggcccgcg cgcgtgcgtg
     5401 ggcgcctgcc gatgctcgag cacctgcccg tcggcggcca cgaggcccgc ggccacggtc
     5461 gtcccgccca cgtccacccc gatgacgacg gttgatactc cctctccccc ctggggagag
     5521 ggccggggtg agggggctcc gtcgcgcaca gccgccctac aggtccccga agtggatcag
     5581 ccggatgccc tcccgctcga tgagggcgcg ggcctccgga tccgtgaggc cggccagctc
     5641 gatgtcccgc tggcgaccgt agcggctgta cgccagatcg tcgtcgaagt agccgggatg
     5701 ggtcatgaac tcggtcacgc cgcccggcag ctggcgcagg tgcgcgagca cgcgctcgga
     5761 cgaccagtag gcgtccgggc ccgactcgcc catgaaatga tccggcgtct tgagcgcgag
     5821 cttgcgcgcc gccgcccgca cctccgcgtc ctgcgtccgc accggcacct tgatggcgcg
     5881 cgcgaagtcc agcaccagat ccaggatcgg ctcgtgccgg ccgacgtgat ggtgcgagtc
     5941 cagatgcgtg gggaaccgtc ccatgatctt ccggaactcg tcgatctgca tcccgagctc
     6001 gatgcgcgcc tcgtccggcc tggcgcgctg ggcggcctcc cgggcgtcgc gggtgaactt
     6061 cccttccgcg tcgagcagcg aggccacccg cttcgcgggc gccaccggcg gtcccagcgt
     6121 gaggttcatg tgcagcccca cgcccatctc cgaggccttg agcgcgtcga tgagcgcggg
     6181 gtccaccggc cggttcacga gcagcgtcgt gctcgtcacg atcccgcgcc ggtgcgagtc
     6241 caggatgccg cggctgacac ccgcggtgag gccgtagtcg tcggcgttga cgatgagacg
     6301 ccgggtgggc ttcatcgcga tgggtcctcc atccgggtgc cctgccaagt gccctggaag
     6361 acgtagcgct cgacggtggc gggccacccg gcccgcggca gggtggcttc cgcgtcgagc
     6421 gcgcggatgt gctcgatgcg ctcgtagccg atgcgcggga aggcctgcgt ctcctggccc
     6481 aggatcgcgg gctcgaaccc cgcgtcgtgg agccgggcga gcgccgcctt gacaccgaga
     6541 aaggcgaaca gggtgaacac gagccgtccg cccggtgcca ggtgtgcggg cgcgcccgcg
     6601 atcacccggt cgagcagggc ccagccgtcc gggccgccgt tgtcggccgc cgccgccgcg
     6661 tcggcgcgct cgcgatttgg cggcgtgggc atctggggcg ggctcgtgca gatcaggtcg
     6721 aagacgcgcc cgcgcaccgg cgcgaagccg tcgcccgcga ggacctccac gcgatccgcc
     6781 acgccgttga tcatcgcgtt cgcgcgcgcg caccgcaccg ccgcgtccac cacgtcggtg
     6841 gccaccacgc gggcccccgc gcgcgcggcc agcaccgcgg cgagtccgat cccggtgccg
     6901 atctcgagca tccggtcgcc cgcgcgcacc ggcaggtgac ggcagaacag cagcgagccc
     6961 gccttgggcg gctgcacgcc gtcggccacc gtgaacgtgc agccgccgtg gacgaagagc
     7021 agcgcgcccg agtcggtcac ttgcccatca cccgcaggaa gtagagccgg agggcttcct
     7081 tgagctgcgc gcgggcgccc gcgccggtct gggcgtcctc gagctgctcg atatcgatgg
     7141 acagcggcac gccttccttc ttca
//
LOCUS       DASUFA010000210         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_376835, whole genome shotgun sequence.
ACCESSION   DASUFA010000210 DASUFA010000000
VERSION     DASUFA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376835"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1315)
                     /locus_tag="VFX87_07730"
     CDS             complement(122..1315)
                     /locus_tag="VFX87_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994841.1"
                     /translation="MIRRARAVLAVACGAHFIHDGFSDILYVLLPVWATEFGLSFAQV
                     GLLKTLYTGGMAAFQVPAGILAERRGEARLLVAGTAVTALGFLIAGLTGSFVALLVCL
                     VMAGLGSGCQHPLSSSLVAKAYETGPRRTALGTYNFSGDLGKVAVPAAVALALPWLGW
                     RGAVEVYGIVGLVAAAVILALLARLGAGLRPAPSPPGVAPARAAGSGIRDVRAFTAIS
                     AIHVIDNSTRTGFLTFLPFLLIGKGATVQSVGLALMLVFMGGAVGKFACGALADRVGV
                     IRTVVVTELVTGAGILLLLALPLGACLVALPVLGVALNGTSSVLYGSVADLVSSERRA
                     RSYAIFYTLGIGASALSPFAYGLLSDWGGVRLTLSVVGSVVFLTLPFTLPLRQPSAVA
                     APHSA"
     gene            complement(1331..1846)
                     /locus_tag="VFX87_07735"
     CDS             complement(1331..1846)
                     /locus_tag="VFX87_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994842.1"
                     /translation="MNPQQIFGLQVLLSFLVYGLVARWYVTPRLAPLPLHAALQPLLV
                     LHASRHLGLVFLVPTVVGPALPASFAIPAAYGDLGAGLLALAAIVALRARAGIALSLT
                     WLFNVVGLMDLIHAFYQGLRNDVQLGAAYFIPTFVVPALVITHAMIFRMLVRHPRCPA
                     PAPHLPASSQM"
     gene            complement(1843..3267)
                     /locus_tag="VFX87_07740"
     CDS             complement(1843..3267)
                     /locus_tag="VFX87_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX4994843.1"
                     /translation="MSHAVAAVAPVAAAPPLSPRTRRLLEGPVARTLLVLAAPNVLVM
                     VLQAAVSTLDAVFVGWLGSAALAGVSIVFPLVMLMQTMSAGGMGGGVASAVARALGGG
                     RRADADALVIHAIVIALAMGGAFTLTLELAGPALYRAMGGTDEVLAAAVAYSRVLFGG
                     AVAYWLFNTLGSAIRGSGNMAVPASVMVASGILYLGLAPALILGWGPLPRLGIAGAAT
                     ASVFSFGLGSLVLLAFLCSGRSLVRPALHGRGLRRGLFREILRVGAPGSLNTVLTNLT
                     VVVLTALVGPFGATALAGYGLGARLEYLQIPIVFGLGSGLVTMVGTSIGAGDRARAVR
                     VAWVGSAMAAGITGVIGILAALFPLAWLGLFTADPGVLAAGTRYLTIVGPFYGFFGAG
                     LALYFASQGAGRLLWPLLAGFTRLVIAAVGGWLATRWLGGGLSGIFAALAAALVVFGV
                     INIAAVRRGAWRARAGRPRAGSAR"
     gene            complement(3267..3704)
                     /locus_tag="VFX87_07745"
     CDS             complement(3267..3704)
                     /locus_tag="VFX87_07745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4994844.1"
                     /translation="MPKALDFIRMVQRGEAAPPPIAELIGFTLTEADPGRAVVSFDVG
                     PRHANPMGTLHGGVLCDIADAAMGMAYAAGLDEGETFTTLELKINFLKPVRAGRLIAT
                     GRLVKGGHTVGLLECDVVDDKDRLVARASSTCMTLRGEQAIGR"
     gene            3776..4234
                     /locus_tag="VFX87_07750"
     CDS             3776..4234
                     /locus_tag="VFX87_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4994845.1"
                     /translation="MPKQYNQSCPVAKTLEVIGDRWTLLVVRDLLRGTQRFQDLLGSL
                     PGIAPNILSARLKLMEEHGLIRRTLYSDHPPRAEYALTDRGRELGMVVGALAAWGGRH
                     VHRETQLVHAGCGHPVSVGYYCPDCKEGVRGADVAVTRVRARRGSPAPRG"
     gene            complement(4188..>4701)
                     /locus_tag="VFX87_07755"
     CDS             complement(4188..>4701)
                     /locus_tag="VFX87_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994846.1"
                     /translation="ATRFTLAHPERVRALIVVDSSSAVGLPLSVENLVMRARSIEVTL
                     KGGMDAMAEFAIASNPNVAGRLKLDPGARQEIFDYYRKLTPVGYANALRALLQMDYIT
                     ERLPEIGVPTLLICGDEDPSLEPMRVMEKKVKSSRFVLLSPAGHFGNRDQPEDFNRAV
                     LDFLDALEPA"
BASE COUNT          801 a         1653 c         1742 g          505 t
ORIGIN      
        1 cgccaacggc ggcccggaac cgcacgattc cagattggga actcccgagg ccccaagacg
       61 agaaagcgtg tcgtggtcag aggcccaccg gccgcccccg ccgcccggcc gcgcgccggg
      121 cctaggccga gtgaggcgcg gcgaccgcag acggctgacg gagcggcagg gtgaaaggca
      181 gcgtgaggaa cacgacgctc ccgacgacgc tgagcgtgag ccgcacgccc ccccagtcgc
      241 tgagcagccc gtacgcgaac ggcgacagcg ccgacgcccc gatgccgagc gtgtagaaga
      301 tcgcgtagct ccgcgcgcgc cgctcgctcg agaccagatc cgccacgctg ccgtagagga
      361 cggacgacgt gccgttgagg gccacgccga gcaccggcag cgcgacgaga caggcgccca
      421 gcggcagggc cagcaggagc aggatgcccg cgccggtgac gagctcggtg accaccacgg
      481 tgcggatcac cccgacccga tccgcgaggg ccccgcacgc gaacttcccc accgcgccgc
      541 ccatgaagac gagcatgagc gcgagcccga ccgactgcac cgtcgcgccc ttgccgatca
      601 gcaggaaggg caggaaggtg aggaacccgg tgcgggtgga gttgtcgatg acgtggatcg
      661 cggagatcgc ggtgaaggcg cgcacgtcgc ggatgcccga gccggccgcg cgcgcggggg
      721 cgaccccggg cggcgacggc gccggacgga ggcccgcgcc caggcgcgcg agcagcgcca
      781 ggatgaccgc ggcggccacg agcccgacga tgccatagac ctccaccgcg ccccgccagc
      841 cgagccaggg cagcgcgagc gccaccgccg cgggcaccgc gaccttgccg agatcgccgg
      901 agaagttgta ggtgccgagc gcggtgcgtc gcggcccggt ctcgtaggcc ttcgccacca
      961 gcgaggacga gagcgggtgc tggcagccgg agccgagacc cgccatcacg aggcagacga
     1021 gcagggcgac gaagctgccg gtcaggccgg cgatcaggaa gccgagcgcg gtgaccgcgg
     1081 tgcccgccac cagcagccgc gcctcgccgc gccgctccgc gagaatgccc gcgggcacct
     1141 ggaaggcggc catgcccccg gtgtagagcg tcttgagcag cccgacctgt gcgaagctca
     1201 ggccgaactc ggtcgcccac accggcaaca gcacgtacag gatgtcggag aagccgtcgt
     1261 ggatgaagtg cgccccgcag gcgacggcga ggacggcgcg ggcgcggcga atcatcgccc
     1321 gtcgattgtt tcacatctgt gacgacgcgg gcaaatgcgg agcaggggcc gggcagcgcg
     1381 gatggcggac cagcatccgg aagatcatgg cgtgcgtgat caccagcgcc ggcaccacga
     1441 aggtggggat gaagtacgcg gccccgagct gcacgtcgtt cctgagcccc tggtagaacg
     1501 cgtggatcag gtccatcagg ccgaccacgt tgaagagcca cgtcagggac agggcgatgc
     1561 cggcgcgcgc tctgagcgcg acgatcgcgg ccagggcgag caggcccgcc ccgagatcgc
     1621 cataggcggc cggaatcgca aagctggccg gaagagcggg gccgaccacg gtcggcacga
     1681 ggaagaccag ccccaggtgg cgcgaggcgt ggaggacgag cagcggctgc agggcggcgt
     1741 ggagcggcag cggagccagc cggggcgtca cgtaccaccg ggccacgagg ccgtacacca
     1801 ggaagctcag caggacctgg agcccgaaga tctgctgagg attcatcgcg cggatccggc
     1861 gcgaggccgc ccggcgcggg cccgccaggc gccccggcgg accgccgcga tgttgatgac
     1921 gccgaacacc acgagcgccg ccgccagggc cgcgaagatc ccggagaggc cgccgccgag
     1981 ccagcgcgtg gcgagccagc cgcccaccgc ggctatgacc agacgggtga atccggcgag
     2041 gagaggccag agcaggcggc ccgcgccctg cgaggcgaag tagagcgcga gccccgcgcc
     2101 gaagaagccg tagaacgggc cgacgatcgt gaggtagcgc gtgcccgcgg cgagcacgcc
     2161 cgggtcggcg gtgaagagcc cgagccaggc cagcgggaac agtgcggcga ggatcccgat
     2221 cacaccggtg atgcccgcgg ccatcgcgga gccgacccac gcgacgcgca cggcgcgcgc
     2281 gcggtcgccc gccccgatgc tggtgccgac catggtgacg agccccgacc ccaggccgaa
     2341 cacgatcggg atctggagat actcgaggcg cgcgcccagg ccatagccgg cgagcgccgt
     2401 cgcgccgaag ggcccgacca gcgcggtcag gaccacgacg gtcagattcg tcaagaccgt
     2461 gttgagcgac ccgggggcgc cgacacgcag gatctcccgg aagaggccgc gccgcagacc
     2521 ccggccatgc agggcgggcc gcaccaggct ccggccggag cagagaaacg cgagcagcac
     2581 gaggcttccg aggccgaagg agaacacgct cgcggtggcg gcgcccgcga tccccagacg
     2641 gggcagcggg ccccagccga ggatcagggc gggggcgagc cccaggtaga ggattccgct
     2701 cgccaccatc accgaggcgg gcaccgccat gttgccgctg ccgcggatcg cgctgccgag
     2761 cgtattgaag agccagtagg cgacggcccc gccgaagagg acgcgagagt aggccaccgc
     2821 ggccgcgaga acctcgtccg tcccccccat cgctcggtag agcgcggggc cggccagctc
     2881 gagcgtgagc gtgaacgccc cgcccatggc gagcgcgatc acgatggcgt ggatgacgag
     2941 cgcgtcggca tcggcgcggc ggcctccgcc gagggcgcgc gccaccgcgg aggccacgcc
     3001 gccgcccatc ccgcccgccg acatcgtctg catcagcatg accagcggga acacgatcga
     3061 gaccccggcc agcgccgccg agccgagcca gccgacgaag accgcgtcga gcgtgctcac
     3121 cgccgcctgc agcaccatca cgagcacgtt gggcgcggcc agcacgagca gggtgcgcgc
     3181 caccggcccc tcgaggagcc ggcgggtgcg cggggacagc ggcggggcgg cggcgaccgg
     3241 ggccaccgcg gcgacggcgt ggctcactag cgtcctatcg cttgctcgcc tcggagcgtc
     3301 atgcaggtgc tggaagcgcg cgcgacgagg cggtccttgt cgtccaccac gtcgcactcg
     3361 agcagcccca ccgtgtgccc gcccttgacg aggcgcccgg tggcgatgag ccggcccgcc
     3421 cgcaccggct tgaggaagtt gatcttgagc tcgagcgtgg tgaaggtctc gccctcgtcg
     3481 aggcccgccg cgtaggccat gcccatggct gcgtccgcga tgtcgcacag cacgccgccg
     3541 tggagcgtgc ccatggggtt ggcgtgccgc gggcccacgt cgaacgagac gaccgcgcgg
     3601 ccgggatccg cctcggtgag cgtgaagccg atcagctcgg cgatcggggg cggggccgct
     3661 tccccgcgct gcaccatccg gatgaagtcg agcgctttcg gcatcggggt cctccagtga
     3721 ctattaaaat aatagtcact tgcgatcctg tcaagggggc tcgtatactt cggatatgcc
     3781 gaagcaatac aaccagtcct gcccggtggc gaagacgctc gaggtcatcg gcgaccgctg
     3841 gacgctcctg gtggtgcgcg atctgctgcg cggcacccag cgcttccagg atctgctggg
     3901 cagcctgccc ggcatcgccc ccaacatcct gtccgcgcgg ctcaagctga tggaggagca
     3961 cgggttgatc cgccgcacgc tctactcgga ccatccgccg cgggcggagt acgccctcac
     4021 cgaccggggc cgggagctgg ggatggtggt gggggcgctc gccgcgtggg gcgggcgcca
     4081 cgtgcaccgc gagacgcagc tcgtgcacgc cgggtgcggg cacccggtgt cggtcggcta
     4141 ctactgcccg gactgcaagg agggagtccg cggggcggac gtcgcggtca cgcgggttcg
     4201 agcgcgtcga ggaagtccag caccgcgcgg ttgaagtcct ccggctgatc gcggttgccg
     4261 aagtggccgg ccggggagag cagcacgaag cgtgacgact tgaccttctt ctccatcacc
     4321 cgcatcggct cgagcgaggg atcctcgtcg ccgcagatca acagggtggg cacgccgatc
     4381 tcggggagcc gctcggtgat gtagtccatc tgcagcagcg cgcgcagggc gttcgcgtag
     4441 ccgaccgggg tcagcttccg gtagtagtcg aagatctcct ggcgcgcgcc cgggtccagc
     4501 ttgagccggc ccgccacgtt cgggttcgac gcgatggcga actcggccat ggcgtccatc
     4561 ccgcccttga gcgtcacctc gatcgaccgc gcgcgcatca ccaggttctc gaccgagagc
     4621 ggcaggccca ccgcggagga cgagtccacc acgatgagag cccgcacccg ctcggggtgc
     4681 gccagcgtga agcgggtggc g
//
LOCUS       DASUFA010000211         7990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1636905, whole genome shotgun sequence.
ACCESSION   DASUFA010000211 DASUFA010000000
VERSION     DASUFA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636905"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFX87_07760"
     CDS             <1..225
                     /locus_tag="VFX87_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20 family protein"
                     /protein_id="HEX4994847.1"
                     /translation="MPGSRVVPKMTTGGTDAKFFRWQGVPAYGFGLQSLRIPYTDYPL
                     MFHGHNERVDTESLKLSAMMWETLAREFLG"
     gene            complement(230..1582)
                     /locus_tag="VFX87_07765"
     CDS             complement(230..1582)
                     /locus_tag="VFX87_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4994848.1"
                     /translation="MTTDAKMINHLWTHKTQDHPFLSDEELVIDRAEGVWVWTEQGKK
                     LLDGFAGLAVVNVGHGRREIAEAIAEQTVRLAYYPTTRQFSNRPAAELAAKLASLTPG
                     DLRYTMFAVSGSEANERSMQIARQYWLATGKPGKYKVISLKGGYHGATMGTFAVCGLP
                     NLSQAYAPLEVPGFVKVAPPHPFRDLAGGTEAELIARRIAELREAIVREGPDTVSAVI
                     MEPVLSSGGFIMPPIGWLRAVRALCDELDVLMIADEVITGFGRTGRWFACEHDQVVPD
                     LMSVAKGITSGYIPLSASIARGRLADAFSDTTTQENVHPNTYAAHPVACAAALANLRI
                     MEQDKLVANAAAMGTRLLEGLRRAVGKHPIVGEVRGRGLLVCVDLVQPDGSGKPLDAK
                     LVAELDRKAWDRGAIIYARGTVLRLAPPLCITAAEVDELVGVVADCVDSLQRDVAAAR
                     "
     gene            1783..4485
                     /locus_tag="VFX87_07770"
     CDS             1783..4485
                     /locus_tag="VFX87_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4994849.1"
                     /translation="MSPESRAAMQDEAALHARLRWLEQENARLLEERWTRLQETRALT
                     GIGRLLSERLDPDVVGERIAESLRSLLGGGSAVVYRLDDDSANLLAMAVAHGPATPTA
                     GWRPVREIGSGAVGLAMRERRTITSPDVLADARIDLPPALRAHMDEGPDRAILAVPLL
                     TQDRLIGVLAVRTATGTVFDARAVQLAEALADQAAITLEHARLFAEQERRRREAEVLA
                     DLARTIGAAPELATVLRRVAEAAQELCGSDGSVIGLRIPESDAVRLRYWTSPWYGALG
                     ETRVEPGQGLGGRVLEERRPLRTSDYLRDPRIGSHHAERIRQLRIQAKMAVPILIDDR
                     VEGLLYVDNRTARAFTDQHESVLVRLAAQAAIAIRNAQILEAEQIARGTAERLVRALR
                     ESQDRFQFVARATNDAVWDWDLVSDSLWWNEGVNTLFGYTPEQVGPDVTWWYETIHPD
                     DRERVKSDINAAVERGAESWSAEYRYRRVDGTYASVFDRGYVLHDGDGRPTRMIGAMM
                     DITQRLELEEELRQAQKMEAVGRLAGGVAHDFNNLLTIITGRAHLVLGRLTGDDPARR
                     SIEVIQKTADRAAALTRQLLAFSRKQVLQRKVLDLNTMVADVSTMLRRLIGEDVDLLL
                     TLGPGAGRVNADRAQLEQALMNLAVNARDAMPQSGTLGLETDHVRLGAVPSDRPDALP
                     PGPYAVLRVMDTGTGMDAATQARIFEPFFTTKEPGKGTGLGLSMVHGIVRQHGGAIHV
                     RSVVGGGTTFEIYLPQIETAADTGGADDPGAPLPASGQETILLVEDEADVRALAREVL
                     ERQGYTVLEASDGAQAVQVYEKEGARIDLILTDVVMPRMSGREMVDRVHATRPDMRVL
                     YMSGYTGDAIVRHGVLDASMLLLGKPFTPGALIAKVREVLDRPR"
     gene            complement(4571..5962)
                     /locus_tag="VFX87_07775"
     CDS             complement(4571..5962)
                     /locus_tag="VFX87_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX4994850.1"
                     /translation="MRRPLSGFRWLIPLLGSLAVLAAPSPEGLSLEGQRVLAVIVLAI
                     GLWGFETLPPGVTGVVAILALVFTHAVPSIREGLVGFADPIAYFLIGVLTLGVAVARS
                     GLAERVAHVLLARGRGRAGALYAHLLLALPLLTLLLPSATTRTGILVHVYDQAFTLGR
                     VPPGAPLARAIMLVLNSVNRLSSTILLTGGITPIVAAGLIGTMSWTRWFVLMSVPYVA
                     LITLAAAAIYLRYRHGFHAQVQVPPRREPRPLSGVEWRTALITAGAGLLWLTDSVHHW
                     HPTLPALLAWVFFLMPGVGVLTWGQFEREVGWTNFLVIAASLSLAQALSRSGASAWLA
                     GGVVGAAHAVAESPWMVVGVLLVASSLVRLLIPGITGFLATTIPVAMAVGTTAGLNPI
                     LCGLIVTIAGDAVLYYPAQSPSSLVVYERGHLTAGEIFGFGLLMTALAALVVLAVAIP
                     YWALIGEPLASGR"
     gene            complement(5975..6154)
                     /locus_tag="VFX87_07780"
     CDS             complement(5975..6154)
                     /locus_tag="VFX87_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994851.1"
                     /translation="MRLFWIPLAALLLAGCVIVPLAPDAYAPPPPRPRFTYRYPVYPA
                     YPYWHAPRYYDRGRW"
     gene            complement(6256..7446)
                     /locus_tag="VFX87_07785"
     CDS             complement(6256..7446)
                     /locus_tag="VFX87_07785"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994852.1"
                     /translation="MLQGIRVLDLSRVIAGPYCAALLGDLGADVIKLERPGRGDDLRA
                     LRGDGRMSASFAAVNRNKRGIAVDLQKPEGARLAFDLARRADVVIENFLPGVAEKLGL
                     GYPAVSAENPAVVYASVTGFGQTGPLARKPGYNTIAQGMSGLMALTGMPGDPPTRVGG
                     SVSDVAASYMAFGMVNAALVHRFRTGEGQHVDVSLVSASLGLLPDPVAIFFDTGERPK
                     RAGNRNLNLSPAEAFQAKDGFVNVVLMNTEQWGRFCQALGDPAMETDPRFADNPARLA
                     NHAAFKARVEAILGTGSATDWVARLEAGGIAAGPIYEFHEVFEDPQVRHLGLVAEVDQ
                     PGVGKVKMLTFPGRASGTPPRIDRPAPLLGQHTAEVLGELGIAREEIDRLAAAGVIVL
                     GEQP"
     gene            7613..>7990
                     /locus_tag="VFX87_07790"
     CDS             7613..>7990
                     /locus_tag="VFX87_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4994853.1"
                     /translation="MRYQRISADCHIDLPWIPPDLFTANASAALRDRMPYVKDGPDGP
                     FWTARNGTSFGLWGGVGPAGSKYEPGKHHRVDVMAATGLYDDGRKGIRRPTDPLLRAK
                     DMDRDGVQAEVIYGILGAATRLND"
BASE COUNT         1245 a         2835 c         2880 g         1030 t
ORIGIN      
        1 atgccgggca gccgcgtggt gcccaagatg accaccggcg gcaccgacgc caagttcttc
       61 cgctggcaag gggtgcccgc ctacggcttc ggcctgcagt cgctccgcat cccctacacc
      121 gactacccgc tgatgttcca cggccacaac gagcgcgtgg acacggagag cctcaagctc
      181 tccgcgatga tgtgggagac gctcgcgcgc gagttcctcg gctagcccgc tagcgggcgg
      241 ccgcgacgtc gcgctggagc gagtcgacgc agtcggcgac cacgccgacc agctcgtcca
      301 cctccgcggc ggtgatgcag agcggcggcg ccaggcgaag caccgtcccg cgggcgtaga
      361 tgatggcgcc gcggtcccag gccttgcggt cgagctcggc caccagcttg gcatcgagcg
      421 gcttgccgga gccgtcgggc tgcaccaagt ccacgcagac gaggaggccg cggccgcgga
      481 cctcgcccac gatcgggtgc ttgcccaccg cccggcgcag gccctcgagc agccgggtgc
      541 ccatcgcggc cgcgttcgcc accagcttgt cctgctccat gatgcgcagg ttggcgagcg
      601 cggccgcgca ggccaccgga tgggccgcgt aggtgttggg gtgcacgttc tcctgggtgg
      661 tggtgtcgga gaaggcgtcg gcgagccgcc cgcgggcgat gctggccgag agcgggatgt
      721 agcccgaggt gatgcccttg gccaccgaca tgaggtcggg cacgacctgg tcgtgctcgc
      781 acgcgaacca ccggccggtg cggccgaagc cggtgatgac ctcgtcggcg atcatcagca
      841 cgtccagctc gtcgcagagg gcgcgcaccg cgcggagcca gccgatgggc ggcatgatga
      901 agccgcccga ggagagcacc ggctccatga tcaccgcgga caccgtgtcc ggcccttcgc
      961 gcacgatggc ttcgcgcagc tcggcgatcc ggcgcgcgat cagctccgcc tcggtcccgc
     1021 ccgcgagatc gcggaacggg tgcggcggcg cgaccttgac gaacccgggc acctcgagcg
     1081 gggcgtaggc ctgggagaga ttcggcaggc cgcagaccgc gaaggtaccc atggtggcgc
     1141 cgtggtagcc gcccttgagc gagatcacct tgtacttgcc gggcttgccg gtggccagcc
     1201 agtactgccg cgcgatctgc atcgagcgct cgttggcctc ggagccgctc accgcgaaca
     1261 tcgtgtagcg caggtcgccg ggcgtgagcg acgcgagctt cgcggccagc tccgccgccg
     1321 gccgattgga aaactgtctg gtggtcgggt agtaggccag ccgcaccgtc tgctcggcga
     1381 tcgcctccgc gatctcgcgg cgaccgtggc ccacgttgac gaccgccagc ccagcaaagc
     1441 cgtccaggag cttcttgccc tgctccgtcc acacccagac gccttccgcc cgatcgatca
     1501 ccagctcctc gtcggacagg aacggatgat cctgcgtctt gtgcgtccag aggtgattga
     1561 tcatcttcgc gtcggtcgtc atggcggcga gtctcccgga tgaggtgagc agctggcgac
     1621 ggtgagcgga tcgtagcaga gcgcccgggg ccttgtcccc gccggggtgc tcattttcag
     1681 tcacggactg acagtttgcg cagacctatg ctcaggatct gccggtattt cagctacttg
     1741 cccgtggcgt ggtccttgct gcctaacgac cggatgccac ccatgagccc cgagagccgc
     1801 gcggccatgc aggatgaggc ggcgctgcac gcccgcctgc gctggctcga gcaggagaac
     1861 gcgcgcctcc tcgaggagcg gtggacgcgc ctgcaggaga cccgggccct caccggcatc
     1921 ggccggctgc tgtccgagcg gctcgacccc gacgtggtcg gcgagcgcat cgccgagagc
     1981 ctccgctcac tgctgggcgg cggctccgcg gtcgtctacc ggctcgacga cgactccgcg
     2041 aacctgctcg cgatggcggt ggcgcacggg cccgcgacgc cgaccgcggg ctggcgtccg
     2101 gtgcgcgaga tcggatcggg cgcggtcggc ctcgccatgc gcgagcgccg caccatcacg
     2161 agcccggacg tcctggccga cgcgcggatc gacctccccc cggccttgcg cgcgcacatg
     2221 gacgaggggc ccgaccgggc catcctcgcg gtgccgctgc tcacgcagga ccggctcatc
     2281 ggcgtgctcg cggtccgcac cgcgaccggc accgtcttcg acgcgcgcgc ggtgcagctc
     2341 gccgaggccc tggccgacca ggccgcgatc accctcgagc acgcccggct cttcgcagag
     2401 caggagcggc gccgacgcga ggcggaggtg ctggccgacc tggcccgcac gatcggggcc
     2461 gcccccgagc tcgccaccgt gctccgccgg gtcgccgagg ccgcgcagga gctctgcggt
     2521 agcgacggct cggtgatcgg cctgcgcatc cccgagtccg acgcggtgcg cctccgctac
     2581 tggacctctc cgtggtacgg cgcgctcggc gagacccggg tcgagcccgg ccaggggctc
     2641 ggcgggcggg ttctcgagga gcgccgcccc ctgcgcacga gcgactacct gcgcgacccc
     2701 cgcatcggtt cccatcacgc cgagcgcatc cggcagctgc gcatccaggc caagatggcg
     2761 gtgccgatcc tcatcgacga ccgggtcgag ggcctgctct acgtcgacaa ccgcacggcc
     2821 cgcgcgttca ccgaccagca cgagtcggtc ctggtgcggc tggccgcgca ggccgcgatc
     2881 gccatccgga acgcccagat cctcgaggcc gagcagatcg cccgcggcac cgcggagcgg
     2941 ctggtccggg cgctgcggga gagccaggat cgctttcagt tcgtggcgcg cgccaccaac
     3001 gacgcggtgt gggactggga cctcgtgagc gattcgctgt ggtggaacga gggcgtcaac
     3061 accctgttcg gctacacccc ggagcaggtc ggtccggacg tcacgtggtg gtacgagacg
     3121 atccatccgg acgatcgcga gcgcgtgaag agcgacatca acgcggcggt ggagcgcggc
     3181 gccgagagct ggagcgccga gtatcgctac cgccgcgtcg acggcacgta cgccagcgtc
     3241 ttcgaccggg gctacgtgct gcacgacgga gacggccggc ccacgcgcat gatcggcgcg
     3301 atgatggaca tcacccagcg cctggagctc gaggaagagc tccgccaggc ccagaagatg
     3361 gaggcggtcg gccgcctcgc gggcggcgtc gcgcacgact tcaacaacct gctcacgatc
     3421 atcaccggcc gcgcccacct ggtcctcggg cggctcaccg gtgacgaccc ggcgcgccgc
     3481 agcatcgagg tgatccagaa gacggcggac cgcgccgccg ccctcacgcg ccagctcctg
     3541 gccttcagcc gcaagcaggt gctccagcgg aaggtcctcg acctcaacac catggtggcg
     3601 gacgtgagca cgatgctgcg gcggctcatc ggcgaggacg tggacctgct gctgacgctg
     3661 gggccgggcg ccgggcgggt caacgcggat cgggcccagc tcgagcaggc gctgatgaat
     3721 ctggccgtga acgcgcgcga cgcgatgccc caaagcggca cgctcggcct cgagaccgac
     3781 cacgtgcggc tcggggcggt cccatccgat cggccggacg cgctcccgcc cgggccgtac
     3841 gcggtgctcc gcgtgatgga caccggcacc ggcatggacg cggcgaccca ggcccgcatc
     3901 ttcgaaccgt tcttcacgac caaggagccc ggcaagggca ccggcctcgg gctctcgatg
     3961 gtgcacggga tcgtgcgcca gcacggcggc gcgatccacg tgcgcagcgt ggtgggcggc
     4021 ggcacgacct tcgagatcta cctgccccaa atcgagacgg cggccgacac cggcggcgcc
     4081 gacgatcccg gcgcgcccct gccggcctcc ggccaggaga cgatcctgct ggtggaggac
     4141 gaggccgacg tgcgggccct cgcccgcgag gtgctggagc ggcagggcta cacggtgctg
     4201 gaggcgagcg acggcgccca ggccgtccag gtctacgaga aggaaggcgc gcggatcgac
     4261 ctcatcctca ccgacgtggt gatgccgcgc atgagcgggc gcgagatggt ggaccgcgtg
     4321 cacgcgaccc ggcccgacat gcgcgtgctg tacatgtcgg gctataccgg cgacgccatc
     4381 gtccgccacg gcgtgctcga cgcgagcatg ctgctgctcg gcaagccgtt cacgcccggc
     4441 gccctgatcg ccaaggtccg cgaagtcctc gaccggcccc gataggaacc tcggagcggg
     4501 ctccgcccct tccgaacctc ccccccagag caggttgcac gggcaaagcc cgcgctcgaa
     4561 gcgggccggc tcatcggccg ctggccagcg gctcgcctat cagcgcccag tagggaatcg
     4621 ccaccgccag caccacgagg gcagccagcg cggtcatcag gagcccgaag ccgaagatct
     4681 cgccggcggt gaggtgtccg cgctcgtaga ccaccagcga cgaggggctc tgggctgggt
     4741 agtacaggac cgcgtcgccc gcgatggtga cgatgagccc gcagaggatg gggttcaggc
     4801 ccgcggtggt gcccaccgcc atcgccaccg ggatcgtggt cgcaaggaag ccggtgatgc
     4861 ccgggatcag gagccgcacc agcgaggagg cgacgagcag cacgcccacc accatccagg
     4921 gtgactcggc caccgcgtgg gcggccccca ccacgccgcc ggccagccag gcgctcgcac
     4981 cggagcgcga gagcgcctgg gccagggaca gcgacgcagc gatgacgagg aaattcgtcc
     5041 agcccacttc ccgctcgaac tgcccccagg tgagcacgcc cacgccgggc atgagaaaga
     5101 acacccaggc cagcagcgcg ggcagcgtgg gatgccagtg gtggacggaa tcggtcagcc
     5161 agaggagccc cgctcccgcg gtgatcagcg cggtgcgcca ctcgactccg gagagcgggc
     5221 gcggctcgcg ccggggcggc acctgcacct gcgcgtggaa gccgtggcgg taccgcaggt
     5281 agatggcggc ggcggcgagg gtgatcaggg ccacgtacgg cacgctcatg agcacgaacc
     5341 agcgcgtcca cgacatcgtg ccgatcaggc cggcggccac gatgggcgtg atgccgccgg
     5401 ttaacaatat tgtggacgac agccggttca ccgagttcag caccagcatg atggcgcggg
     5461 cgagcggcgc gccgggcggc acgcggccga gcgtgaaggc ctggtcgtag acgtggacga
     5521 ggatgccggt gcgcgtggtg gccgacggga gcagcagcgt gagcagcggc agcgcgagca
     5581 gcaggtgcgc gtagagcgcg ccggcccggc cccgcccgcg cgcgagcagc acgtgagcca
     5641 cccgctcggc gaggccgctg cgcgccaccg ccacgcccag ggtcagcacg ccgatcagga
     5701 agtacgcgat gggatccgcg aagcctacga gcccctcgcg gatgctcggc accgcgtgcg
     5761 tgaagaccag ggccaggatt gcgaccacgc cggtgacgcc gggggggaga gtctcgaagc
     5821 cccagagccc gatggcgagc acgatcaccg cgagaacgcg ctggccttcc aagctcaggc
     5881 cctcgggcga gggcgccgcc agcaccgcca ggcttccgag gagcggaatc agccagcgga
     5941 agccggacag tgggcggcgc acccgccgag gatactacca gcggccgcga tcgtagtagc
     6001 gcggcgcgtg ccagtacgga taggcgggat agacggggta gcggtaggtg aagcgcggcc
     6061 gcggaggcgg cggcgcgtag gcgtccggtg cgagcggcac gatgacgcat ccggccagca
     6121 gcagcgcggc caggggaatc cagaacagtc gcatgacggt gtcctcctcg cccgatcaga
     6181 cgaaggggga cgccgtgaat attccttttc ttgcgtgtaa gcgcgcggcg ggcccgccgc
     6241 gtctaagcag actggctagg gctgctcgcc gagcacgatg acgccggccg cggcgaggcg
     6301 atcgatctcc tcgcgcgcga tcccgagctc gccgagcacc tcggcggtgt gctggccgag
     6361 cagcggcgcg gggcgatcga tacgcggcgg ggtgccggag gcgcggcccg ggaacgtgag
     6421 catcttcacc ttgcccacgc cgggctgatc cacctcggcc acgagcccca ggtggcgcac
     6481 ctgcgggtcc tcgaagacct cgtggaactc gtagatgggg cccgccgcga tgccgcccgc
     6541 ttccagacgc gccacccagt cggtggccga ccccgtgccc aggatggcct ccacgcgcgc
     6601 cttgaacgcc gcatgattgg caagccgtgc cggattgtcg gcgaaacgcg gatcggtctc
     6661 catggccgga tcgccgagag cctggcaaaa gcgcccccac tgctccgtgt tcatcagcac
     6721 cacgttgacg aagccgtcct tggcctggaa ggcttccgcg ggcgagaggt tcaggttgcg
     6781 gttacccgcg cgcttgggcc gctcgccggt gtcgaagaag atcgcgaccg ggtccggcag
     6841 gagacccagg ctcgcggaca cgaggctcac gtccacgtgc tggccttccc cggtgcggaa
     6901 gcgatgcacg agcgcggcgt tgaccatgcc gaaggccatg taggacgcgg ccacgtcgga
     6961 gaccgagccg cccacgcgcg tcggcggatc gccgggcatg ccggtgagcg ccatcagccc
     7021 gctcatgccc tgcgcgatcg tgttgtagcc gggcttccgc gcgagcggcc cggtctggcc
     7081 gaagccggtc accgaggcgt agaccacggc aggattctcc gcgctcaccg ccgggtagcc
     7141 caggccgagc ttctcggcga cgccgggcag gaagttctcg atgaccacat ccgcgcgccg
     7201 cgccaggtcg aaggcgagcc gggcgccctc gggcttctgc agatccaccg cgatcccgcg
     7261 cttgttacgg ttgacggccg cgaaggaggc gctcatccgg ccatcgccgc ggagggcgcg
     7321 caggtcgtcg ccgcggccgg gccgctccag cttgatgacg tcggcgccca ggtcgccgag
     7381 cagggccgcg cagtagggcc cggcgatgac gcgggagaga tcgaggaccc ggatgccctg
     7441 gagcatggcg gcccgggcat ggtgccgcgt ccggaggccc ggagtcaagg cgcgagaggc
     7501 cgggcccgca tactgtcggg gggccgccgg tggccagttg acgtgggccc cgccggtggc
     7561 taggctctgt ccacatacga ggagcgaccc ggtaccggcc aaagggggcg tcatgcgcta
     7621 ccaacggatc tccgccgact gccacatcga cctgccctgg atcccgcccg atctgttcac
     7681 cgcgaacgcc tcggcggcac tcagggaccg catgccctac gtgaaggacg gccccgacgg
     7741 ccccttctgg accgccagga acggcaccag cttcggcctc tggggcggcg tcggcccggc
     7801 cggatccaag tacgagcccg gcaagcacca ccgcgtggac gtgatggcag ctacgggtct
     7861 ttacgatgac ggccggaagg ggatacgtcg tcctaccgat ccgcttctgc gcgcgaagga
     7921 catggaccgc gacggcgtgc aggccgaggt catctacggc atcctgggcg cggccactcg
     7981 cctgaacgac
//
LOCUS       DASUFA010000212         6873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_625576, whole genome shotgun sequence.
ACCESSION   DASUFA010000212 DASUFA010000000
VERSION     DASUFA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625576"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="VFX87_07795"
     CDS             complement(<1..1763)
                     /locus_tag="VFX87_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994854.1"
                     /translation="MTRAWLPPALLAAYAVAFGVRALSGGLLVFDDHPGQLYRLAHAI
                     TLGLAPWRFNPGWWAGYAELQYYPPGFAWLGALIHLVAGGALEVASVYQILLWLAWLL
                     PGVAVYALLRRALGDPWLALPGALVALTLSAGSRSGVEEGLRWGLIAARLGWGVLPLL
                     ALCLLRWAEGAARPPLASAALIAAVILLHPAHAPAAVALVALAALHSAPRRRRLGEAA
                     LILAGALGLAAVWLLPLLAHLRMALPLAWADATLFALGRRLGSQPVLLALALLSLVGW
                     RWRARLPIVPDGGARWLLGLAPAMLVVIALDALVVTPLGVAWLPADRLMDGLLLGLVL
                     GGAAGFAALAAGRARSAVAAVVALAVCVPLAWGPDEPGLTLWPRAREWPREQEVTRGL
                     RMDALWEAIRQAPPGRVLFLRAALPLDWRPEWWRPHTHVTALTPLRTGRAIVGGTFTH
                     PSPVAGLVYTGSAAPRPLAMLAEQRDGQSLFGRALEGLPPEAFNDLARSLGIGLVVAL
                     EEDVGKSLFLTDNQSLKSLSRIGSFRLYALAGSAAGPVPIGTQRWRISLPASGPGWVS
                     LPIAYSPLWMARAAGAPLAVRR"
     gene            1916..3172
                     /gene="thrC"
                     /locus_tag="VFX87_07800"
     CDS             1916..3172
                     /gene="thrC"
                     /locus_tag="VFX87_07800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX4994855.1"
                     /translation="MDRLKGLKCRECGRGYPSSPSHVCEFCFGPLEVDYDYGLIRGRI
                     SRARIEAGPKSIWRYADLLPLDLVDGLPPVGAAVGFTPLVRARNLADEWGVRELYVKN
                     DSVSHPTCSFKDRVVAMAIGKAREFGFDTVACASTGNLANSVAAHAAEARLACYVFIP
                     ADLEPGKVIATLIYAPTVIEVQGTYDEVNRLCAEVGDRYHWAFVNINLRPYYAEGSKT
                     YGYEIAEQLGWRAPAHVVAPCAGGSLLTKIAKAIRELQMLGLIPESRTRMYAAQAAGC
                     GPIVTMIKKDTDRLEPVRPQTICKSLAIGNPADGYYAYRAVKDSGGYGEHATDEEIIE
                     GMRLLARTEGIFAETAGGVTVAATRKLIEAGRIPRDEPIVMCVTGNGLKTPDALQDRL
                     HSNVTIQPSLTAFDQALADLKSKTGT"
     gene            3195..3473
                     /locus_tag="VFX87_07805"
     CDS             3195..3473
                     /locus_tag="VFX87_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX4994856.1"
                     /translation="MAVLVRIPTPLRALTKGAAEVQADAGTVTDLIEALERQFPGLKE
                     RLVEDGGELRRFINIYINQEDIRFLQGATTTLKQGDEVSIVPAIAGGR"
     gene            3499..4662
                     /locus_tag="VFX87_07810"
     CDS             3499..4662
                     /locus_tag="VFX87_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4994857.1"
                     /translation="MISKRVQGFTESVIREMTRVNNQYDGINLAQGMPNFPPPRELIE
                     AAHRAIDGDFHQYAITWGTPSLRHAIAEKYRKFYGMDVEADRNVTVCCGSTETMLATL
                     LAVLNPGDEVIIFEPFYENYGPGCIIAGAEPVWVPLEAPDFSFDPDRLSRAVSARTRA
                     IVLNSPNNPSGKVFSRAELQVIADVCIKHDLLAITDEIYEHIVYDGLGHTPIATLPGM
                     ADRTITISGISKSYSVTGWRVGYAVASPELSLGIRRAHDFITVGAPHPLQEAAVTALG
                     LPDSYYVRLRESYQARRDLLLGKVEEAGFVAYKPQGAYYILTEAEHFLKRFDCRDDHE
                     FALYLIREIGVATVPGSSFYAHGELGRTKIRFCFPKTDDILIEAGRRLQKLAR"
     gene            4659..5951
                     /locus_tag="VFX87_07815"
     CDS             4659..5951
                     /locus_tag="VFX87_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX4994858.1"
                     /translation="MSLREAARSVFDAALRAADVHPLVRRALADVRPAARGRVLVVGA
                     GKASGAMAAAAERVLGDRIADGLVVVKDRYLAETTRVRLVEAGHPVPDARGEAAAHRI
                     RALAESAGGDDLLLVLVSGGGSALTPAPVPPITLADKQAMTRLMLAAGANINQLNAVR
                     KHCSLLKGGQLARAAAPARVHALLLSDVIGDPLDVIASGPTAPDASTFGEALAILDRF
                     ELRSRAPVAIVDRLERGARGEVPETPKPEDPVFRGVTNTVIGNNLLVVEAAAAQARAL
                     GYAPHVLTRALEGEARDVARTLVGLAREIRAGRGPVAPPACLIAGGETTVTVRGTGSG
                     GRCQEWTLAAALELDGVDGIVALAAGTDGTDGPTDAAGAVADGATAKRARERGDDARA
                     RLSRNDSNPVFAALGDLVVTGPTNTNLLDLYLLLVEAR"
     gene            complement(6037..>6873)
                     /locus_tag="VFX87_07820"
     CDS             complement(6037..>6873)
                     /locus_tag="VFX87_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX4994859.1"
                     /translation="DYVMLINAADSRADLTGWVTINNQSGATYRDAALKLVAGDINRA
                     ASRDDSRRAMEMAAKAASPAVADREFKSEGFFEYHLYTLDGRTTIKESQTKQLTLLSA
                     SDFPVDKRFIYFGAASYHRTQYGVPISNQKVGVYLDIRNTKENRLGAPLPKGKVRVYK
                     ADASGSQQLIGEDWIDHTPKDERVRIKMGEAFDVVGERVQKDWKRIGGNLYEVEWEIT
                     LRNHKKEAVTVEVIEPMPGDWEVLRATHPHEKLEAFTARWRIPVPKEGAATLGYRTRV
                     RF"
BASE COUNT         1051 a         2512 c         2409 g          901 t
ORIGIN      
        1 cgacgcaccg ccagcggtgc gcccgcggcg cgcgccatcc agagcggcga gtaggcgatc
       61 ggcagcgaga cccagccggg cccggacgcg ggcagggaga tccgccagcg ctgggtcccg
      121 atcggcaccg gcccggccgc agagcccgcc agcgcgtaga gcctgaacga gcctatgcgg
      181 gagaggctct taagcgattg attgtcagtc agaaataggg atttccccac gtcctcctcg
      241 agcgcgacga ccaggccgat cccgaggctg cgtgcgaggt cgttgaacgc ctccggcggg
      301 agcccctcga gcgcgcggcc gaacagactc tggccgtctc gctgctcggc cagcatcgcg
      361 aggggacgcg gagccgccga gcccgtgtag accagcccgg ccaccggcga cggatgcgtg
      421 aaggtcccgc cgacgatcgc gcgcccggtg cggagggggg tgagggcggt gacgtgcgtg
      481 tggggccgcc accattcggg acgccagtcg agcggcagcg cggcgcgaag gaagagcacg
      541 cggccgggcg gcgcctgacg gatcgcctcc cagagcgcat ccatccggag gccgcgggtc
      601 acctcctgct cccttggcca ctcgcgcgcc cgcggccaga gcgtgagccc gggctcatcc
      661 gggccccagg ccagcggtac gcacaccgcc agggcgacca cggcggcgac ggcggagcgc
      721 gcgcgccccg cggcaagagc cgcgaatccc gcggcgccac ccagcacgag cccgagcagg
      781 agcccgtcca tcagccgatc cgcggggagc cacgccacgc cgagcggcgt cacgacgagc
      841 gcatcgagcg cgatcaccac gagcatggcc ggcgccaggc ccagcagcca gcgcgcgccc
      901 ccatcgggca cgatggggag ccgcgcgcgc cagcgccacc cgacgaggct cagcagtgcc
      961 agcgcgagca gcaccggctg cgacccgagc cgccggccga gggcaaagag cgtcgcgtcg
     1021 gcccaggcca ggggcaaggc catgcgcagg tgcgcgagca gcggcagcag ccagaccgcg
     1081 gccagcccga gcgcgcccgc gaggatgagc gcggcctcgc cgaggcggcg acggcgcggg
     1141 gccgagtgaa gggccgccag cgccaccagc gccacggcgg ccggcgcgtg ggccggatgc
     1201 agcaggatca ccgccgcgat cagcgcggcg ctcgcgagcg gcgggcgggc ggcgccctcc
     1261 gcccagcgca gcaggcataa cgcgagcagc ggcaggacgc cccagcccag gcgggccgcg
     1321 atcagccccc agcgcaggcc ctcctccacg ccgctccggg agcccgccga cagcgtgagc
     1381 gcgacgaggg cgccggggag cgcgagccac ggatcgccga gcgcgcggcg cagcagcgcg
     1441 tacaccgcaa cgcccggcag cagccacgcg agccagagga ggatctgata gacgctggcc
     1501 acctcgagcg cgccgcccgc caccagatgg atcagcgcgc cgagccaggc gaagccgggc
     1561 gggtagtact gcagctccgc gtagccggcc caccagcccg gattgaagcg ccacggggcc
     1621 aatcccagcg tgatcgcgtg cgcgaggcgg tagagctgcc ccggatgatc gtcgaagacg
     1681 agcaggccgc cggaaagcgc gcgcaccccg aaggccacgg cgtaggcggc gagcagcgcg
     1741 ggaggcagcc aggcgcgggt cacgggtgct gatgatacgg catcgcgttg acaacccgcg
     1801 gcgcggagtg ttaagtaaac gcattcgttt tccgctgggt cggtgatccg ggacggccaa
     1861 cccggatcgt gcagctcatt ccggggctca aatcccctac cgattggaga ggccattgga
     1921 cagattgaag ggcttgaagt gccgggagtg tggccgcggc tacccttcct ccccgtctca
     1981 cgtctgcgag ttctgcttcg gtcccctcga ggtcgactac gactacgggc tgatccgcgg
     2041 gcggatcagc cgcgcgcgca tcgaggccgg ccccaaatcg atctggcgct acgccgatct
     2101 cctgccgctc gatctggtgg acggcctacc gccggtcggc gccgcggtgg ggttcacccc
     2161 gctggtgcgc gcgcgcaacc tggcggacga gtggggcgtc cgggagctct acgtcaagaa
     2221 cgactcggtc tcgcacccga cgtgctcgtt caaggatcgc gtggtcgcga tggcgatcgg
     2281 caaggcccgg gagttcggct tcgacacggt ggcgtgcgcc tccacgggca acctcgccaa
     2341 ctcggtcgcg gcccacgccg cggaggcgcg cctggcgtgc tacgtcttca tcccggccga
     2401 tctcgagccg ggcaaggtca tcgcgacgct gatctacgcg cccaccgtga tcgaggtgca
     2461 ggggacctac gacgaggtca accgcctctg cgccgaggtc ggggaccggt atcactgggc
     2521 cttcgtgaac atcaacctcc ggccctacta cgcggagggc tcgaagacct acgggtacga
     2581 gatcgccgag cagctcggct ggcgcgcgcc cgcccacgtg gtggcgccct gcgcgggcgg
     2641 ctcgctcctc accaagatcg ccaaggcgat ccgggagctc cagatgctcg ggctgatccc
     2701 cgagagccgc acccgcatgt acgcggcgca ggccgccggc tgcgggccca tcgtcaccat
     2761 gatcaagaag gacaccgacc ggctcgagcc ggtgcggccc cagaccatct gcaagtcgct
     2821 cgcgatcggc aacccggccg acgggtacta cgcctaccgg gccgtcaagg attcgggcgg
     2881 ctacggcgag cacgccaccg acgaggagat catcgagggc atgcggctgc tcgcgcgcac
     2941 cgagggcatc ttcgccgaga cggcgggggg cgtgacggtg gcggcaaccc ggaagctcat
     3001 cgaggccggg cgcatccccc gcgacgagcc catcgtgatg tgcgtcaccg gcaacggctt
     3061 gaagacaccg gacgccctgc aagaccggct gcatagtaac gtcaccatcc agccctcgct
     3121 caccgcgttc gatcaagcgc tggccgacct caagtccaag accgggacgt agcccggacg
     3181 aaggagacca tcacatggcc gttctggtcc gcattcccac gccgctccgg gccctcacca
     3241 agggtgcggc cgaggtacag gccgacgcgg gcaccgtcac cgacctgatc gaggctctcg
     3301 agcgccagtt ccccgggctc aaggagcgcc tcgtcgagga cggcggcgag ctccgccggt
     3361 tcatcaacat ctacatcaac caggaagaca tccggttcct gcagggcgcg accaccacgc
     3421 tcaagcaggg cgacgaggtc tccatcgtcc cggccatcgc gggcggccgg tagcgcgcca
     3481 cgcccgctcc cgcgccgcat gatctccaag cgcgtccagg gcttcaccga gtcggtgatc
     3541 cgggagatga cccgggtcaa caaccagtac gacggcatca acctcgcgca ggggatgccg
     3601 aacttcccgc cgccccggga gctgatcgag gcggctcacc gcgccatcga cggcgacttc
     3661 caccagtacg cgatcacctg gggcacgccc tcgctccggc acgccatcgc ggagaagtat
     3721 cggaagttct acggcatgga cgtcgaggcc gaccgaaacg tgacggtctg ctgcggctcc
     3781 accgagacca tgctcgccac cctgctggcg gtgctgaacc cgggcgacga ggtcatcatc
     3841 ttcgagccct tctacgagaa ctacgggccg gggtgcatca tcgcgggggc cgagccggtg
     3901 tgggtcccgc tggaggcgcc ggacttctcg ttcgatcccg accggctgtc ccgggcggtc
     3961 agcgcgcgca cccgcgccat cgtcttgaac agcccgaaca atccctccgg caaggtgttc
     4021 tcccgcgcgg agctgcaggt catcgccgat gtctgcatca agcacgacct gctcgcgatc
     4081 accgacgaga tctacgagca catcgtctac gacggcctcg gccacacccc gatcgcgacg
     4141 ctgcccggca tggccgaccg caccatcacg atctcgggca tctcgaagtc ctactcggtg
     4201 accggctggc gggtgggcta cgcggtcgcc agccccgagc tgtcgctcgg catccggcgg
     4261 gcccacgact tcatcacggt gggcgcgccg catccgctcc aggaagcggc ggtgaccgcc
     4321 ctcggcctgc ccgactcgta ctacgtccgc ctgcgcgaga gctaccaggc ccggcgcgat
     4381 ctcctgctgg gcaaggtgga ggaggcgggc ttcgtggcct acaagcccca gggcgcctac
     4441 tacatcctca ccgaggccga gcacttcctg aagcggttcg actgccgcga cgaccacgag
     4501 tttgccctct acctgatccg cgagatcggc gtggcgacgg tgcccggctc gtccttctac
     4561 gcgcacggcg agctgggccg gacgaagatc aggttctgct tccccaagac ggacgacatc
     4621 ctgatcgagg cgggccgccg gctgcagaag ctggcgcgtt gagcctccgc gaggcggcgc
     4681 gctcggtctt cgacgccgcg ctgcgcgccg ccgacgtcca tcctctggtg cgccgcgccc
     4741 tcgccgacgt gcggccggcc gctcgcggcc gcgtgctcgt ggtgggggcg ggaaaggcct
     4801 cgggtgccat ggccgccgcc gcagagaggg tgctcggcga ccgcatcgcg gacgggctcg
     4861 tcgtggtcaa ggacaggtat ctcgccgaga cgacccgcgt tcgcctggtc gaggccggcc
     4921 acccggtccc cgacgcccgc ggcgaggccg ccgcccaccg gatccgggcg ctcgccgagt
     4981 ccgccggagg cgacgacctg ctgctggtcc tggtctcggg cggaggctcg gcgctcacgc
     5041 ccgcgccggt gccccccatc accctggccg acaagcaggc catgacgcgc ctgatgctcg
     5101 ccgccggcgc caacatcaac cagctcaacg cggtccggaa gcactgctcg ctgctgaagg
     5161 gcggccagct cgcccgggcc gcggcgcccg ctcgcgtgca cgcgctgctg ctctccgacg
     5221 tcatcggcga ccccctcgac gtcatcgcct ccggccccac cgcgcccgac gcgtccacgt
     5281 tcggggaggc gctcgcgatc ctcgaccgct tcgagctccg ctcgcgggcg cccgtggcca
     5341 tcgtggatcg gctcgagcgc ggagcccgcg gcgaggtccc ggagaccccg aagcccgagg
     5401 atccggtgtt ccgcggtgtg accaacacgg tgatcggcaa caacctcctg gtcgtcgagg
     5461 cggcggccgc gcaggcgcgc gcgctcggct acgcgccgca cgtcctgacc cgcgcgctcg
     5521 aaggcgaggc gcgggacgtg gcgcgcacgc tcgtcggcct ggctcgcgag atccgggccg
     5581 ggcgagggcc ggtggcgccg cccgcatgct tgatcgcggg cggggagacc acggtgaccg
     5641 tgcgcgggac gggcagtggc ggccgctgtc aggagtggac cctggcggcg gcgctcgagc
     5701 tcgacggcgt ggacggcatc gtcgcgttgg cggcgggaac cgacggcacc gacgggccca
     5761 ccgacgccgc gggggcggtg gcggacggcg ccaccgcgaa gcgtgcgcgg gagcgcggcg
     5821 acgatgcgcg ggcccggctg tcgcggaacg actcgaatcc ggtgttcgcg gcgctgggcg
     5881 acctggtggt cacgggcccg accaacacca acctgctcga cctctacctg ctcctcgtcg
     5941 aagcgcgctg aatcccgcgc gtctcggcat cgggcaccgg ccgctgcggc tcagggatgc
     6001 ctgagccgca gcgcctgtca cccggaaccc gtccgcctag aagcgcacgc gggtgcggta
     6061 gccgagcgtg gcggctccct ccttgggcac cgggatacgc caccgggcgg tgaaggcctc
     6121 gagcttctcg tgcggatggg tggcgcgcag gacctcccag tcgccgggca tgggctcgat
     6181 cacctcgacg gtgaccgcct ccttcttgtg gttgcggagg gtgatctccc actcgacctc
     6241 gtagagattg ccgccgatcc gcttccagtc cttctgcacg cgctcgccca ccacatcgaa
     6301 ggcctcgccc atcttgatcc gcacccgctc gtccttcggg gtgtggtcga tccagtcctc
     6361 gccgatgagc tgctggctcc cggaggcgtc cgccttgtac acgcgcacct tgcccttggg
     6421 caggggcgcg cccaggcggt tctccttcgt attgcggatg tccaggtaga cacccacctt
     6481 ctggttcgag atcggcaccc cgtactgcgt gcggtggtag ctcgcggcgc cgaagtagat
     6541 gaagcgcttg tccaccggga agtccgacgc cgacaggagc gtgagctgct tggtctggct
     6601 ctccttgatg gtggtacgcc cgtcgagggt gtagaggtgg tactcgaaaa agccctccga
     6661 cttgaactcg cggtcggcca ccgcggggga cgccgccttc gcggccatct ccatcgcgcg
     6721 ccgcgagtcg tcccggctcg cggcccggtt gatgtccccc gccaccagct tcaacgccgc
     6781 gtctcggtag gtcgccccgc tctggttgtt gatggtgacc cagccggtga gatccgcgcg
     6841 ggagtcggcc gcgttgatga gcatcacgta gtc
//
LOCUS       DASUFA010000213         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1421976, whole genome shotgun sequence.
ACCESSION   DASUFA010000213 DASUFA010000000
VERSION     DASUFA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421976"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="VFX87_07825"
     CDS             <1..1836
                     /locus_tag="VFX87_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4994860.1"
                     /translation="VLERRGARVALLTTAGFRDLIEIGRTKRNIPALFIPTFVRPKPV
                     VERKHRFEVTERLGPDGAVLVPLDPASVERALDGALAADAEAVAVCLLHAYLNSAHER
                     LVADAVKGRAPGLPVSCSADVVAEYREFERFSTTVLNAYLQPLMEGYLTSLEERLLAT
                     GYTHGVLTVASSGGMMTTDTARRLPIKTIFSGPAGGVSQACFVGAAAGIRDFITYDMG
                     GTSTDVCLVRDLQPLMTADAMVGAFPVKVSQIDMHTVGAGGGSIAWLDVDGSLQVGPR
                     SAGARPGPAAYGLGGTEPAVTDANVVLGRIGTRRRLGGSIGIDPALARRAVADLAARM
                     EPPLGVEALAEGIVTIAVARMTSAIREISIQRGHDPRDFTLIAFGGAGPMHALAMADE
                     IGIPRVLVPRHPGNFSALGLLAADIKHDDVRTRVGPLRERLPALRLAFAEMETAARQQ
                     LEREGFAPEQQKLLRTLDLRYRGQAFELNVPAPDTELQIDRIEADFHRQHRDTYGHAN
                     PQAAIELVNARLTAYGLVPKPAAERHAAPGAALDRALVERRPVWFEGRAHDCPVWDRE
                     RLPERAELRGPGIVEEFGATTVVPPGWRGALDPHGNLRFEREALA"
     gene            1833..>3081
                     /locus_tag="VFX87_07830"
     CDS             1833..>3081
                     /locus_tag="VFX87_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4994861.1"
                     /translation="MSASTVDPITLEVLRETFSSIVREMRVTLVRTAYSSILYEGEDF
                     SCVLMDGDAQLVAMSKGQDHPLHIVPISWSMKSVKEKFGDDIHPGDIFLHNDPYTGGT
                     HLNDVAMIYPLFADGRLFVFPVVRAHWGDVGGMSPGSLSGDATEIFQEGVRIPPIRIA
                     DRGTPNQAALDLIFHNMRGPRERKGDFDAMIGTCRKAAERVEALAARYGAPVVRAAVV
                     ELMDRAEQRMRRAIGALPDGEYSYEAHLESGRVRLEPLSIRARIIVAGETITVDLTGT
                     SPQTAAPTNVGPAMAPTGAFTIIKSFLDPGTDVNSGAFRPLTVITPRGTVVNADPPAP
                     CGGMVEVKYCVETAVMGALAQALDGRVAGDLKGGGNHCYVGGPDPRTGETFIFYEYPA
                     GGTGGFHGGDGSNTVRTWTESDMT"
BASE COUNT          435 a         1172 c         1052 g          422 t
ORIGIN      
        1 gtgctcgagc gtcgcggggc ccgcgtcgcg ctgctgacca ccgccggctt ccgcgatctc
       61 atcgagatcg ggcggaccaa gcggaatatc ccggcgctgt tcatcccgac cttcgtgcgg
      121 ccgaagccgg tggtggagcg caagcaccgc ttcgaggtga ccgagcggct cggcccggac
      181 ggtgcggtgc tggtccccct cgaccccgcc tccgtcgagc gcgcgctcga cggcgcgctc
      241 gccgccgacg cggaggcggt ggcggtgtgc ctcctccacg cctatctcaa ctccgcgcac
      301 gagcgcctcg tggccgacgc ggtgaagggc cgcgcccccg gcctgcccgt ctcctgctcg
      361 gccgacgtgg tggccgagta ccgcgagttc gagcgcttct cgaccaccgt gctgaacgcc
      421 tatctccagc cgctgatgga aggctacctg acctcgctcg aggagcgcct gctcgccacc
      481 ggctacacgc acggcgtgct cacggtcgcc tcgagcggcg gcatgatgac caccgacacc
      541 gcgcgccgcc tgccgatcaa gaccatcttc tcggggccgg cgggcggggt gagccaggcc
      601 tgcttcgtgg gcgcggccgc gggcatccgc gacttcatca cctacgacat gggcggcacc
      661 tccaccgacg tctgcctcgt gcgcgatctt cagccgctga tgaccgccga cgccatggtg
      721 ggcgccttcc cggtcaaggt ctcgcagatc gacatgcaca cggtgggcgc gggcggcggc
      781 tccatcgcct ggctcgacgt ggacggcagc ctccaggtcg gcccgcgcag cgcgggcgcc
      841 aggccgggcc cggcggccta cggcctgggc ggcaccgagc cggcggtcac cgacgccaac
      901 gtggtgctcg ggcgcatcgg cacgcgccgg cgcctgggcg gcagcatcgg catcgatccc
      961 gcgctggcgc gccgggccgt cgcggatctg gccgcgcgca tggagccgcc cctcggcgtc
     1021 gaggccctcg ccgagggcat cgtcaccatc gcggtcgccc gcatgacctc cgcgatccgc
     1081 gagatctcca tccagcgtgg ccacgatccg cgcgacttca ccctgatcgc gttcggaggc
     1141 gcggggccga tgcacgccct cgccatggcc gacgagatcg gcatcccgcg ggtcctcgtc
     1201 ccgcggcacc ccggcaactt ctcggctctg gggctgctgg ccgccgacat caagcacgac
     1261 gacgtacgga cgcgcgtggg cccgctccgc gagcgcctcc ccgccctgcg cctggccttc
     1321 gcggagatgg agaccgccgc ccgccagcag ctcgagcggg aagggttcgc gcccgagcag
     1381 cagaagctcc tgcgcacgct ggatctccgc taccgcggcc aggccttcga gctcaacgtc
     1441 ccggcccccg acaccgagct gcagatcgac cgcatcgaag ccgacttcca tcgccagcat
     1501 cgcgacacct acggccatgc gaatccgcag gccgccatcg agctggtgaa cgcgcgcctc
     1561 accgcctacg ggctcgtgcc caagccggcc gcagagcggc acgcggcacc gggcgcggcc
     1621 ctcgaccgcg cgctggtcga gcgccggccg gtctggttcg agggccgcgc gcacgactgc
     1681 ccggtgtggg accgggagcg cctgcccgag cgcgccgagc tgcgcgggcc cggcatcgtg
     1741 gaggagttcg gggccaccac cgtggtgccg ccgggctggc gcggcgcatt ggacccccac
     1801 ggcaacctcc gcttcgagcg ggaggccctc gcgtgagcgc ctccaccgtg gacccaatca
     1861 ctctggaagt cctgcgcgag accttctcct ccatcgtgcg cgagatgcgc gtcaccctgg
     1921 tgcgcaccgc ctactcctcg atcctctacg agggcgagga cttctcctgc gtgctcatgg
     1981 acggcgacgc gcagctcgtc gcgatgtcca aggggcagga ccatccgctt cacatcgtcc
     2041 cgatcagctg gtcgatgaag tcggtgaagg agaagttcgg cgacgacata cacccgggcg
     2101 acattttcct gcacaacgac ccctacaccg gcggcaccca cctgaacgac gtggccatga
     2161 tctatccgct cttcgcggat gggcggctct tcgtattccc ggtggtgcgc gcgcactggg
     2221 gcgatgtggg cggaatgagc ccgggcagcc tctccggcga cgccaccgag atcttccagg
     2281 aaggcgtccg cattccgccc atccggatcg cggaccgcgg gacgcccaac caggccgcgc
     2341 tcgacctcat cttccacaac atgcgcgggc cccgcgagcg gaagggcgac ttcgacgcca
     2401 tgatcggcac gtgccgcaag gcggccgagc gggtggaggc cctggccgcg cgctacggcg
     2461 ccccggtcgt ccgcgccgcg gtggtggagc tgatggaccg cgccgagcag cgcatgcggc
     2521 gcgcgatcgg cgccctgccc gacggcgagt actcctacga ggcgcacctc gagagcgggc
     2581 gcgtgcggct cgagcccctg agcatccggg cgcgcatcat cgtcgcgggc gagacgatca
     2641 cggtggatct caccgggacc tcgccccaga ccgcggcacc caccaacgtg ggtcccgcga
     2701 tggccccgac cggcgccttc accatcatca agtcgttcct ggatcccggc accgacgtga
     2761 actcgggcgc cttccgcccg ctgaccgtga tcaccccccg cggaaccgtg gtcaacgccg
     2821 atccgcccgc gccgtgcggc ggcatggtgg aagtgaagta ctgcgtcgag accgcggtga
     2881 tgggcgcgct ggcccaggcc ctcgacggcc gggtggccgg cgatctcaag ggcggcggca
     2941 atcactgcta cgtgggtggg cccgacccgc ggaccggcga gaccttcatc ttctacgagt
     3001 acccggcggg cggcaccggc ggcttccacg gcggcgacgg cagcaacacc gttcgcacgt
     3061 ggaccgagag cgacatgacc a
//
LOCUS       DASUFA010000214        12329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1637574, whole genome shotgun sequence.
ACCESSION   DASUFA010000214 DASUFA010000000
VERSION     DASUFA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637574"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFX87_07835"
     CDS             complement(<1..216)
                     /locus_tag="VFX87_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994862.1"
                     /translation="MPLTSLTGRTAVVTGGASGIGRALSLLFAREGADVVVADLDEAG
                     MAETVAGVGQAGRRGLAVKTDVSRLADV"
     gene            526..3087
                     /locus_tag="VFX87_07840"
     CDS             526..3087
                     /locus_tag="VFX87_07840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX4994863.1"
                     /translation="MDYRLPATVVPSRYDIRLEPDLEAATFAGEETVAITVTAPVTEI
                     VLNAAELSIQSVTVITADGVTVEGSAALDEADERARLLFPSPIPAGQHRLTLRFTGIL
                     NDRLHGFYRSTYKDAAGASHVIAATQFEATDARRGFPCWDEPALKAVFGVTLVVPERL
                     AAISNTRVVSETPTGAGKKTVVFADSIRMSTYLVAFVVGELEATPPVMVGPTPLRVWC
                     VPGKLHLTRFALEIGAFSLDYFERYYGMPYPGDKLDMLAIPDFAAGAMENLGAITYRE
                     TALLVDETAASHTERERVADVVAHEIAHMWFGDLVTMLWWNGIWLNEAFATFMELLAV
                     DAWKPEWKRWTTFGVSRAAAMGVDGLHNTRPIEFEVRAPRDCEAMFDLLTYEKGGSVL
                     RMLEQYLSGEVFRDGVRLYLERHRFGNAETTDLWKALGDASRLPIPEVMNGWIFRPGY
                     PLVIVEPEGVGVRLSQRRFTYLPSEGADAERWQIPLRLRASVKRGFVDKPILLSNEDL
                     SVPLPASPDWVVANAGGHGFYRVQYAPPLLRKLTGALKKLAPIERFNLVSDAFALAQA
                     AMMPAADYLDLTARFVEETDRNVWTALIGSLGYVNRLIADDSRAHLEALARVRVEGAV
                     ERLGWEAQPDEDELERQLRGDLLRALGTLGNDPAAQERARSLYARYREDESVVDANVL
                     PALIAIVAAAGGETEYEEFLQRFKAARTPQEEQRYLYALAGFRPPELVRRTLEKTTNG
                     EVRSQDAPFLMRSLLVGVHSRGLAWDYLKANWQTMDRQYPGSAYRRMYEGVTALVSPE
                     WEQDVKAFFETNRIVLGGKTLEQYLEQLRIAVRLQEREAAALAAYLAKPPRPPRR"
     gene            complement(3084..3515)
                     /locus_tag="VFX87_07845"
     CDS             complement(3084..3515)
                     /locus_tag="VFX87_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994864.1"
                     /translation="MAAKMPDPARHGVTTNRELSQPNEVERALRHRSLIVTLTFSALV
                     GIGHGPARAADSTLMDAATQQVEQGAQQIGRGQVLVGTGELAKGIGKTVVEGAKFTGQ
                     KLTEAGRAAGPDATTAWRRTKEGAVAFGSSVRDFFVDLFGG"
     gene            3545..4396
                     /locus_tag="VFX87_07850"
     CDS             3545..4396
                     /locus_tag="VFX87_07850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4994865.1"
                     /translation="MKYGVFQFSTDYAIRIDDLAREAEARGFESLFVPEHTHIPVSRK
                     SPWPGGADLPREYWHTVDPFVGLAAAAAVTTRLKLGTGICLVIERDPIVLAKEVASLD
                     YLSRGRVLFGIGGGWNAEEMAHHGTDFDTRWKVLRERIAAMQEIWTKDEAEYHGKFVN
                     FDKLWSYPKPVQKPHPPILMGGAGPHARQRAADFGGHWLPIGGRSYSEPLAETIADLR
                     ARAEKAGQDPGAVTVSIFGAPTDEARLAGLRDAGVLRAIFGLPPAGRDQVLPLLDKYA
                     GLMSRVG"
     gene            complement(4411..5661)
                     /locus_tag="VFX87_07855"
     CDS             complement(4411..5661)
                     /locus_tag="VFX87_07855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994866.1"
                     /translation="MSLAPGPPGPAPSPQSESLASQRLWLAFATMLLVAGITNTFPVF
                     LPALLAEFGGSRAATASTASLLWVGGALLSPLAGYLVARWNPRKLVSLGLGLVALGML
                     LGSLAPTLPLFLVAMGLGGGIGLGLTGMTTHAALIADAYVRRRGLATGIAFGGSMAAY
                     ALAAPAQWIITRWGWRPAFWCYAAAIVALIPWVWRTHPTRLASVADRGAVRLGATTVT
                     ASSIVRSAGFWSLLVMFTTPPLFGYLATTQHTLYFTARGFTAGEASLLLAIGGVLAGC
                     GRALAGLVADRFGGPTAGFLSFSCSLLGMLCLIAMEVRPLWVLAAGYVLFLFLPLGSR
                     ASIVTVLLSRIAPPAHYGFIFGLLGIGNNLGAALGPWLSGVIFDHTGGYLAIYLWALG
                     IALIGLAALTVFTLTTRAGAAIRA"
     gene            complement(5658..5855)
                     /gene="rpsU"
                     /locus_tag="VFX87_07860"
     CDS             complement(5658..5855)
                     /gene="rpsU"
                     /locus_tag="VFX87_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX4994867.1"
                     /translation="MRIEVHDNQVESALKTMKKLMLKDGLFKEMKKRAYYEKPSVKRK
                     RKQAEARKKRRKAMKRAETDD"
     gene            5956..6996
                     /locus_tag="VFX87_07865"
     CDS             5956..6996
                     /locus_tag="VFX87_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX4994868.1"
                     /translation="MMVPPARVELALVPTPLLKLDRLSHRLGVELYVKRDDLTGLLES
                     GNKVRKLEFLVGEALQQGADTLVTRGTPDSNGCRAVAAVAARLGLRAVLAIQGERPAA
                     DDGNLLLARLLGAETRYYPDLQPDLGPALADAATEALAAEVRARGGRPYVIPESGAGE
                     LGALGYIECAVELSEQIHHGAPRFDTIVITAGSGGSHAGLLMGKQLAGLPGEIVGVPV
                     TAPAERVREAIVRTVGTAIQRFGFAIEVPKAVHLLDGYQGAGPADATDEELGVIVRLA
                     REEGLLLDPVYTAKGFRGLVETLARDPKALGQRVCFIHTGGLFGLFPFRDRLSRLVWP
                     GEAQPKASRADP"
     gene            complement(6998..7762)
                     /locus_tag="VFX87_07870"
     CDS             complement(6998..7762)
                     /locus_tag="VFX87_07870"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX4994869.1"
                     /translation="MKIKVLGTYGSEGPGQRPTAFLVDDRVLVDAGTVGGALSVPEQI
                     QIQHALITHAHLDHTAGLAFLTDTLAMVAPDRRVTACSIAPVVESLRTHAFNDTLWPN
                     FTNIPSAAQSVLGFRELPEEGESRVGELWVTPVLVEHTVPTTGFVIHDGETGFVFSGD
                     TGPTKRIWQVAREMRGLKALIVETAFPNRLAMLAKASGHLTPEMLRQEIDKMPPDLPI
                     WIYHIKPQLYQETAEELAKIDPSRIHILDQGKTYTI"
     gene            complement(7912..8511)
                     /locus_tag="VFX87_07875"
     CDS             complement(7912..8511)
                     /locus_tag="VFX87_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX4994870.1"
                     /translation="MALRHLVPAIALALTACAAPLQSFTDLERARIDEYERAARQVLE
                     SRGIKGPPPAVRIGEDPALAAMARPAAYFTPRTGFGDLGRPGRIMINRAVVADDLIAQ
                     AVLSHELAHFVLGHGDGRCQSHRHECEVEANVASVELLMTGWDLDYSDAVRLQYAYLK
                     SVVLAVRREETIPSGAAGDPCRELEDFAARFKTSSAACD"
     gene            8602..9975
                     /locus_tag="VFX87_07880"
     CDS             8602..9975
                     /locus_tag="VFX87_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4994871.1"
                     /translation="MIFQQILNEESGCLSYLIGCPQAGQAVIVDPARDRVDDYVAMAR
                     RKGLTVSDILETHVHADHVSGNQALAAKTGARIHIHAAANAAFPHDPVEHGREIRIGS
                     VALQVMHVPGHTPDSIAVLVTDLSRGTEPWFVLTGDTLFVGDVGRPDFGGEQAAANLY
                     ASLTERLLRLPDSVEVYPAHGAGSSCGRAMSSKTATTIGFERRFNAALQVPNAEEFVR
                     RLMMGLPPKPPNFDRIIARNRAQALPQNVEPRPLSATQVRELLDKGACVLDVRSPEEY
                     GEAHVPGALNVWIESPQFANRAGLFAPADAPVVLVVASPTDLTRAMDGLGRIGLDAIA
                     GHLQWGMTEWKSQGLPVGRVPQISVHDLATMKDERPDLVVVDVRELVEWDEGHIDGAL
                     HLPMGEAMARKDLVPAGRPKAVVCAGGLRSSLVISALSRAGLTDFYNVIGGMTAWQKG
                     GYGTVKR"
     gene            9972..10637
                     /locus_tag="VFX87_07885"
     CDS             9972..10637
                     /locus_tag="VFX87_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX4994872.1"
                     /translation="MTDESFDYPVMARALILGDKDTVARKTHEGLALSMDPKDLIFRG
                     LIPGMDVVGEKFRRNEYYVPQVLLSARAMYAGLDLLKPLITNAGGAGQNLGVIVIGTA
                     QGDLHDIGKNLVAMMLEGAGFKVINLGRDVAPEKFVAAVQEHSAHIVGISALMTTTMP
                     AMKRTIDALEKAGLRDRVKVMIGGAPVNQAYADEIGADGYAKDSTIAVVRAKELLGVP
                     VNA"
     gene            10637..11581
                     /locus_tag="VFX87_07890"
     CDS             10637..11581
                     /locus_tag="VFX87_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX4994873.1"
                     /translation="MAVRARGWEIVERLRAGERLVFDGGYGTALFDAGLENGDCPELW
                     NDTHPDVVRSIHQGYFDAGSHLVETNTFGGTALKLHEYDERLGHDMGARTRELNEKGV
                     RLARAACPPGGFVAGSIGPTSYLPLDYGVGKNVATDEEYFATFREQAQALAAAGVDCF
                     AVETMMFPQEAAAAIRACKAVADLPVMATMFFQYEELHDRDRTMWGESPADVARQLLA
                     AGADIVGMNCGRGPDRAIVIIREMRQVTDAPLIAYPNAGLPITTGGRTTYELGPEEMV
                     KDYPALLDAGCNIVGACCGSNPEHIRLIADVVRSRRAR"
     gene            11578..>12329
                     /locus_tag="VFX87_07895"
     CDS             11578..>12329
                     /locus_tag="VFX87_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994874.1"
                     /translation="MTAALSVLSPTPAVLEGLPLAIDRDEVLRFQGYKKDVDVPGPQV
                     LAIFEEALAEGLRLLTPRVVYRAVAVTGGAAGRLDAGGERLHIPQIARLWGAVEAVGA
                     GICTVGAGIEARVRELFDAREFPLAVMLDSVGSAAVEGLAEHANDLLCQAGLRAGTKV
                     TNRISPGYAGWDTAEQAALFRLCPGDPIGVCLNEACFMTPGKSISWLVGIGPGARVDH
                     YFTQCRRCWMRDCAYRRAPADTAHRNQLDSSA"
BASE COUNT         1837 a         4266 c         4456 g         1770 t
ORIGIN      
        1 cacgtcggcg agccggctca cgtcggtctt cacggccaga ccgcggcggc ccgcctggcc
       61 cacgccggcc accgtctcgg ccatgcccgc ctcgtcgagg tcggcgacca cgacatcggc
      121 gccctcgcgc gcgaagagca gagagagggc gcgtccgatt ccgctggcac cgccggtcac
      181 caccgcggtg cggcccgtga ggctggtgag aggcacggcg cagagtgtcg cgctcccctc
      241 cgcgtgctgt caaccctcat ccgcacgtgc agatcgggcg cggatcgggg tcacatagca
      301 tctgcggctc tttgcggccc tctggggctg ggcgatcctg gggccggtgg atgggcggca
      361 ggagctgatc gtgatgggct ggcccatggc gggagcaacc gctcggcccg ggggctgccg
      421 gcggtacgcg gggacggcat gtctggtgcg cgcggacgcc tgctggcgcg gggtgtggcc
      481 atctgggtgg ccccgcgcgc cgcctagcgg tggtattctt cgcgaatgga ttatcgtctc
      541 ccggccaccg tggtgccctc gcggtacgat atccggctcg agcccgatct cgaggcggcg
      601 accttcgcgg gcgaggagac ggtcgcgatc accgtgacgg cgccggtgac cgagatcgtc
      661 ctgaacgccg ccgagctctc catccagtcg gtgaccgtga tcaccgccga cggggtgacg
      721 gtggagggga gcgcggcgct cgacgaggcg gacgagcggg cgcgcctgct ctttccgtca
      781 ccgatcccgg ccggtcaaca ccggctgacg ctgcgcttca ccggcatcct gaacgaccgc
      841 ctgcacggct tctaccgcag cacgtacaag gacgcggcgg gtgcctccca cgtcatcgcg
      901 gccacccagt tcgaggccac cgacgcgcgg cgcggcttcc cgtgctggga cgagcccgcg
      961 ctcaaggcgg tgttcggcgt caccctcgtg gtcccggagc ggctggccgc gatctcgaac
     1021 acgcgggtgg tcagcgagac ccccaccggc gcggggaaga agacggtggt cttcgccgac
     1081 agcatccgca tgtccaccta cctggtggcc ttcgtggtgg gcgagctcga ggcgacgccc
     1141 ccggtgatgg tgggcccgac cccgctgcgc gtgtggtgcg tgccgggcaa gctccacctg
     1201 acccgcttcg cgctcgagat cggcgccttc tcgctcgact acttcgagcg ctactacggg
     1261 atgccctatc cgggcgacaa gctcgacatg ctggcgatcc ccgatttcgc ggcgggcgcc
     1321 atggagaacc tgggcgcgat cacgtaccgg gagaccgcac tcctggtgga cgagaccgcg
     1381 gcgtcccaca ccgagcgcga gcgcgtggcg gacgtggtcg cccacgagat cgcccacatg
     1441 tggttcggcg atctcgtcac catgctctgg tggaacggga tctggctgaa cgaggccttc
     1501 gcgaccttca tggagcttct ggcggtggac gcctggaagc ccgagtggaa gcgctggacg
     1561 accttcggcg tgtcccgggc cgccgcgatg ggagtggacg ggctgcacaa cacccggccg
     1621 atcgagttcg aggtgcgggc gccgcgcgac tgcgaggcga tgttcgatct gctcacctac
     1681 gagaagggcg gctcggtcct gcgcatgctc gagcagtacc tgagcggcga ggtcttccgg
     1741 gacggagtgc gcctctacct cgagcggcat cgcttcggca acgccgagac caccgatctc
     1801 tggaaggccc tcggcgacgc ctcgcgcctg ccgatccccg aggtgatgaa cgggtggatc
     1861 ttccggccgg gctacccgct cgtgatcgtc gagcccgagg gcgtgggcgt caggctgagc
     1921 cagcgccgct tcacctacct gcccagcgag ggcgccgacg ccgagcgctg gcagatcccg
     1981 ctgcgcctgc gggcctcggt gaagcgcggc ttcgtggaca agccgatcct gctcagcaac
     2041 gaggatctgt cggtgccgct acccgcgtcg ccggactggg tggtggccaa tgcgggtggg
     2101 cacggcttct accgcgtgca gtacgctccg ccgctgctca ggaagctcac cggggcgctc
     2161 aagaagctcg cgcccatcga gcgcttcaac ctggtcagcg acgccttcgc gctggcccag
     2221 gccgcgatga tgccggcggc cgactacctc gacctcaccg cgcgcttcgt cgaggagacc
     2281 gaccgcaacg tgtggaccgc gctgatcggc tccctcggct acgtgaaccg cctcatcgcg
     2341 gacgactcgc gcgcgcatct cgaggcactg gcacgcgtcc gggtggaggg agcggtggag
     2401 cggctcggct gggaggccca gcccgacgag gacgagctgg agcggcagct acgcggcgac
     2461 ctgctccgcg ctctcgggac cctcggcaac gacccggccg cgcaggagcg cgcccggtcg
     2521 ctctacgcgc gctatcgcga ggacgagtcg gtggtggatg ccaacgtgct gcccgccctg
     2581 atcgcgatcg tggccgccgc gggcggggag accgagtacg aggagttcct ccagcgcttc
     2641 aaggccgcgc gcacgccgca ggaggagcag cgctatctgt acgccctcgc cgggttccgg
     2701 ccgcccgagc tggtgcggcg cacgctcgag aagaccacca acggggaggt gcgcagccag
     2761 gacgcgccct tcctgatgcg gtcgctgctg gtgggcgtgc acagccgagg gctcgcctgg
     2821 gactacctca aggcgaactg gcagacgatg gaccggcagt atccgggcag cgcctatcgc
     2881 cggatgtacg agggggtgac cgcgctcgtc agcccggagt gggagcagga cgtgaaggcc
     2941 ttcttcgaga cgaaccggat cgtgctcggc ggcaagacgc tcgagcagta cctcgagcag
     3001 ctccggatcg cggtgcggct gcaggagcgc gaggcggccg cgctggccgc ctacctcgcc
     3061 aagccgccgc ggcccccgcg ccgttagccg ccgaacaagt cgacgaagaa gtcgcgcacg
     3121 ctcgagccga aggcgacggc gccttccttc gtgcgccgcc acgcggttgt ggcgtccggc
     3181 ccggcggccc ggccggcctc cgtgagcttc tggccggtga acttggcgcc ttccacgacg
     3241 gtcttgccga tgcccttggc gagctctccc gtgcccacga gcacctgacc ccgcccgatc
     3301 tgctgcgcgc cctgctccac ctgctgggtg gccgcatcca tgagcgtgct gtcggcggcc
     3361 cgcgccggcc cgtggccgat cccgacgagc gccgagaacg tgagggtgac gatcagcgaa
     3421 cgatgcctca gagccctctc cacctcgttc ggttggctta attcccgatt cgtggtaaca
     3481 ccgtgccggg ccgggtccgg catcttcgcg gccactccac caactcttcg agcgaggtag
     3541 ggacatgaaa tacggcgtgt tccagttctc caccgactac gcgatccgca tcgacgacct
     3601 cgcgcgcgag gccgaggcgc gcggcttcga gtcgctgttc gtccccgagc acacccacat
     3661 cccggtgagc cgcaagagcc cctggccggg cggggccgac ctgccgcgcg agtactggca
     3721 caccgtggat ccgttcgtgg ggctggccgc cgcggcggcg gtgaccacca ggctcaagct
     3781 cgggaccggg atctgcctgg tgatcgagcg cgaccccatc gtgctggcca aggaggtcgc
     3841 gagcctcgac tatctctcgc ggggccgggt gctcttcggg atcggcggcg ggtggaacgc
     3901 cgaggagatg gcgcatcacg ggaccgactt cgacacccgc tggaaggtgc tgcgcgagcg
     3961 gatcgccgcg atgcaggaga tctggaccaa ggacgaggcg gaataccacg gcaagttcgt
     4021 caacttcgac aagctctggt cgtacccgaa gccggtgcag aagccgcacc cgccgatcct
     4081 gatgggcggc gccggccccc acgcgcggca gcgggcggcg gacttcgggg gccactggct
     4141 gccgatcggc gggcgctcct acagcgagcc cctggcggag acgatcgcgg acctgcgggc
     4201 gcgggccgag aaggcgggcc aggatccagg cgcggtgacc gtctccatct tcggcgctcc
     4261 caccgacgag gcgcgcctgg ccggcctgcg tgacgcgggc gtgctgcgcg cgatcttcgg
     4321 cctgcccccc gcgggccgcg atcaggtcct gccgctcctc gacaagtacg ccgggttgat
     4381 gtcccgtgtc gggtagtccg gtgaaacgag ctacgcgcgg atagccgcgc ccgcccgggt
     4441 cgtaagcgtg aagacggtca gcgccgcgag cccgatgagc gcgatcccta gcgcccagag
     4501 gtagatcgcc aggtaccccc cggtgtgatc gaagatcacg ccggacagcc acggcccgag
     4561 cgccgcgccc agattgttgc cgataccaag caatccgaag atgaagccat agtgcgccgg
     4621 cggcgcgatg cggctcagca ggacggtgac gatgctcgcc cgcgagccga gcggcaggaa
     4681 gagaaagagc acgtagccgg cggcgaggac ccagaggggc cgcacttcca tcgcgatcag
     4741 gcagagcatg cccaggagcg agcaggagaa cgagaggaag cccgcggtcg gcccgccgaa
     4801 gcgatcggcc accaggcccg ccagcgcgcg gccgcagccc gccaggacgc cgccgatggc
     4861 gaggagcagg gatgcctcgc ccgccgtgaa gccgcgcgcg gtgaagtaga gcgtgtgctg
     4921 ggtggtggcg agatacccga agagcggtgg cgtggtgaac atcaccagga ggctccagaa
     4981 cccggccgag cggacgatgc tcgaggcggt caccgtggtc gccccgagcc gcacggcccc
     5041 gcgatccgcg acggacgcca accgcgtcgg gtgcgtacgc cagacccagg ggatcagcgc
     5101 cacgatggcc gccgcgtagc accagaaggc gggccgccag ccccagcgcg tgatgatcca
     5161 ctgcgccggg gccgccaggg cgtaggccgc catggacccg ccgaacgcga tgccggtggc
     5221 gaggccgcgg cgccgcacgt aggcgtcggc gatgagcgcg gcatgagtcg tcatcccggt
     5281 caggccgagg ccgatgccgc cgcccagccc catggccacc aggaagaggg gcagcgtcgg
     5341 cgccaggctt cccagcagca tcccgagcgc caccagcccg aggcccaggc tgaccagctt
     5401 ccgcgggttc cagcgggcga ccaggtagcc ggcgaggggg ctgagcagcg cgccgcccac
     5461 ccagagcagg gaggcggtgg acgcggtggc cgcccgggag cctccgaact cggcaagcag
     5521 ggcgggcagg aacaccggga aggtattggt gattccggcc acgagcagca tcgtggcgaa
     5581 ggcaagccag agacgctggc tggcaaggga ctcggactgc ggggaggggg ccgggcccgg
     5641 ggggcccgga gcgaggctca gtcgtccgtc tcggcgcgct tcatggcctt ccggcgcttc
     5701 ttccgtgcct cggcctgctt gcgcttccgc ttgaccgacg gcttctcgta gtacgcgcgc
     5761 ttcttcatct ccttgaagag cccatccttc agcatgagct tcttcatggt cttgagcgcg
     5821 gactcgacct gattgtcgtg tacctcgata cgcatgacag gcaaagtacc cggagggcgg
     5881 gccgctgtca atgcccacgt gggtgcccac gcttgccacg tgggtgccga cgcttgagtg
     5941 atacgattgc cccccatgat ggtgcccccg gcccgcgtgg agcttgccct ggtgcccacg
     6001 ccgctcctca agctcgatcg cctctcgcac cggctgggcg tcgagctcta cgtcaagcgc
     6061 gacgacctga ccggcctgct ggagtccggc aacaaggtcc gcaagctgga gttcctggtg
     6121 ggcgaggcgc tccagcaggg cgccgatacc ctggtcacgc gcgggacgcc ggactccaac
     6181 ggctgccgcg cggtggccgc ggtggcggcg cggctcgggc tgcgcgcggt gctcgcgatc
     6241 cagggcgagc gcccggctgc ggacgacggc aacctgctgc tcgcccggct gctcggcgcg
     6301 gagacgcgct actatcccga tctacagccc gatctcgggc ccgcgctcgc ggacgcagcc
     6361 acggaggcgc tcgccgccga ggttcgcgcg cgcggcggac ggccctacgt catcccggag
     6421 agcggcgcgg gcgagctggg cgccctcggc tacatcgagt gcgcggtcga gttgtccgag
     6481 cagatccacc acggggctcc ccgcttcgac acgatcgtca tcaccgccgg gagcggcggc
     6541 agccacgcgg gcctgctcat gggcaagcag ctcgccgggc ttccggggga gatcgtgggc
     6601 gtgccggtca ccgcgccggc ggagcgggtg cgcgaggcca tcgtccggac cgtcggcacc
     6661 gcgatccagc gcttcgggtt cgccatcgag gtgcccaagg ccgtccacct gctggacggg
     6721 taccagggcg ccgggccggc cgacgcgacc gacgaggagc tcggcgtgat cgtgcggctc
     6781 gcgcgggagg aggggctgct gctcgatccg gtctacaccg cgaagggctt tcgcgggctg
     6841 gtggagacgc tcgcgcgcga tccgaaggcg ctcgggcagc gcgtctgctt catccacacg
     6901 gggggattgt tcggcctgtt tccgttccgc gaccgcttga gccggctagt gtggccgggc
     6961 gaggcgcagc cgaaggcgag ccgagcggat ccgtagtcta gatggtgtag gtcttgccct
     7021 ggtccaggat gtggatgcgg ctcgggtcga tcttggccag ctcttccgcc gtctcctgat
     7081 agagctgcgg cttgatgtga tagatccaga tcgggagatc gggcggcatc ttgtcgatct
     7141 cctgtcgcag catttccggg gtgagatgcc cggaggcctt cgcgagcatc gccagccggt
     7201 tggggaaggc ggtctccacg atcagcgcct tcagcccgcg catctcgcgc gcgacctgcc
     7261 agatccgctt cgtcgggccg gtgtcgccgc tgaacacgaa gccggtctct ccgtcgtgaa
     7321 tgacgaagcc ggtagtgggg acggtgtgct cgacgagcac cggcgtcacc cacagctcac
     7381 cgacccgcga ttccccctcc tcgggcagct cgcggaagcc cagcacggac tgcgcggccg
     7441 aggggatgtt cgtgaagttc ggccagagcg tgtcgttgaa ggcgtgggtg cggagcgatt
     7501 ccacgacggg cgcgatgctg caggcggtga cccggcggtc gggcgcgacc atcgcgagcg
     7561 tgtccgtgag gaacgcgagc ccggcggtgt ggtccaggtg agcgtgggtg atcagcgcgt
     7621 gctggatctg gatctgctcg ggcaccgaga gggcgccccc gacggtgccc gcgtccacca
     7681 gcacgcggtc gtcgacgagg aaggccgtcg gcctctgccc cggtccttcg ctgccgtagg
     7741 tcccgaggac cttaatcttc aatgaagccc ttcatgaggc cgtgagtccc cccgtttcgg
     7801 gcggttagca cttctgcgga gtctagtcta gcagcgagag ggggtcaatc cgagggaatt
     7861 gggcgggctt cgacgcccgc aaccgagcgt acccaaaggc catcagcgcc gtcagtcgca
     7921 ggcggcggag gaggtcttga agcgggccgc gaagtcctcg agctcccggc aggggtcccc
     7981 ggccgctccg gacgggatcg tctcctcccg ccgaaccgcc aggacgacgc tcttgaggta
     8041 ggcgtattgg aggcggacgg cgtccgagta gtcgaggtcc cagccggtca tcaggagctc
     8101 cacgctcgcg acgttggcct cgacctcgca ctcgtgccgg tggctctggc accggccgtc
     8161 gccgtggccc aggacgaagt gggcgagctc gtggctgagg acggcctggg cgatcaggtc
     8221 atcggcgacc accgcccggt tgatcatgat ccggcccgga cggccgaggt ctccgaagcc
     8281 ggtgcgcggc gtgaagtagg cggccgggcg ggccatcgcc gcgagcgccg gatcttcccc
     8341 gatccgcacc gccggcggtg ggcccttgat cccgcgggat tccaggacct gccgggcggc
     8401 ccgctcgtac tcgtcgatcc gggcccgctc gagatcggtg aaggactgaa gcggcgccgc
     8461 gcaggccgtc agggccaggg cgatcgccgg caccaggtgt cggagagcca tcacctatcg
     8521 agcatgccac gctcggcaat ttctccgctc cgggctctgg cggcgtggcg ctccctccgc
     8581 cccgtgtggc atactttgcc cgtgatcttt caacagatcc tgaacgagga gtccggttgc
     8641 ctgtcctacc tgatcggctg cccgcaggcc gggcaggcgg tcatcgtgga cccggcccgc
     8701 gatcgcgtgg acgactacgt ggcgatggcc cgtcgcaagg gtctgacggt gagcgacatc
     8761 ctcgagaccc acgtgcacgc cgaccacgtg tcgggcaacc aggcgctggc cgccaagacg
     8821 ggagcccgga tccatatcca cgccgcggcc aacgccgcct tcccccacga cccggtggag
     8881 cacggccggg agatccgcat cggcagcgtg gcgctccagg tgatgcacgt gcccggtcat
     8941 accccggaca gcatcgcggt gctggtgacc gatctgagcc gcggcaccga gccctggttc
     9001 gtgctgaccg gtgacacgct cttcgtcggt gacgtgggcc ggcccgactt cggcggcgag
     9061 caggccgcgg ccaacctcta cgcgagcctc accgagcgcc tcctgcggct gcccgacagc
     9121 gtggaggtgt acccggccca cggcgccgga tcgagctgcg ggcgggccat gtcgagcaag
     9181 accgcgacca ccatcggatt cgagcggcgc ttcaacgccg cgctgcaggt gccaaacgcc
     9241 gaggagttcg tccggcgcct catgatgggc ctgccgccca aaccgcccaa cttcgaccgc
     9301 atcatcgcgc gcaatcgcgc ccaggcgctg ccccagaacg tcgagccgcg cccgctctcg
     9361 gcaacccagg tccgggagct gctcgacaag ggcgcctgcg tgctcgacgt gcggagcccc
     9421 gaggagtacg gcgaggccca cgttcccggg gcactgaacg tgtggatcga gagcccgcag
     9481 ttcgccaacc gggccgggct cttcgcgccc gccgacgcgc cggtggtgct ggtggtggcg
     9541 agccccaccg acctcacccg cgcgatggac ggactcggcc gcatcgggct cgacgcgatc
     9601 gccggccatc tgcagtgggg catgaccgag tggaagagcc aggggctgcc ggtcgggcgg
     9661 gtgccgcaga tcagcgtgca cgatctggcg accatgaagg atgagcggcc cgacctcgtg
     9721 gtcgtggacg tgcgcgagct ggtcgagtgg gacgaggggc acatcgacgg cgccctgcac
     9781 ctgcccatgg gcgaggcgat ggcccgcaag gatctggtgc cggcggggcg gccgaaggcg
     9841 gtcgtctgcg cgggcgggct gcgctccagc ctcgtgatca gcgcgctgag ccgcgccggc
     9901 ctcaccgact tctacaacgt catcggcggc atgaccgcgt ggcagaaggg cggctacggc
     9961 acggtgaagc ggtgaccgac gagagcttcg actatccggt gatggcgcgc gccctcatcc
    10021 tgggcgacaa ggacacggtc gccaggaaga cgcatgaagg gctggccctc tcgatggacc
    10081 cgaaggacct gatcttccgc gggctgattc ccggcatgga cgtggtcggc gagaagttcc
    10141 ggcgcaacga gtactacgtc ccccaggtgc tgctctcggc gcgggcgatg tacgcggggc
    10201 tcgacctgct caagcccttg atcaccaacg cgggcggcgc cggccagaac ctgggcgtga
    10261 tcgtgatcgg caccgcgcag ggcgatctgc acgacatcgg caagaacctg gtggcgatga
    10321 tgctcgaggg cgccggcttc aaggtcatca atctgggccg ggacgtggcc cccgagaagt
    10381 tcgtggccgc agtgcaggag cactcggccc acatcgtcgg catctccgcg ctgatgacca
    10441 cgacgatgcc cgcgatgaag cgcaccatcg acgcgctcga gaaggcgggg ctgcgcgatc
    10501 gcgtgaaggt catgatcgga ggggcccccg tgaaccaggc gtacgccgac gagatcggcg
    10561 ccgacggcta cgccaaggat tcgaccatcg cggtggtgcg cgccaaggag ctgctcggcg
    10621 tgccggtgaa cgcgtgatgg cggtccgcgc gcgcggctgg gagatcgtgg agcggctccg
    10681 tgccggcgag cgcctggtct tcgacggcgg ctacggcacc gcgctcttcg acgcgggcct
    10741 cgagaacggc gactgccccg agctctggaa cgacacgcat cccgacgtgg tccggtctat
    10801 ccaccagggc tacttcgacg cgggctcgca tctcgtcgag accaatacct tcgggggcac
    10861 cgcgctcaag ctgcacgagt acgacgagcg cctcgggcac gacatgggcg cgcgcacccg
    10921 cgagctgaac gagaagggcg tgcggctggc ccgggcggcc tgcccgcccg gcggcttcgt
    10981 ggccggctcc atcgggccca ccagctacct gccgctcgac tacggcgtcg gcaagaacgt
    11041 ggccaccgac gaggagtact tcgcgacgtt ccgggaacaa gcccaggccc ttgccgcggc
    11101 gggggtggac tgcttcgcgg tggagaccat gatgttcccg caggaagcgg ccgccgcgat
    11161 ccgggcctgc aaggcggtgg cggacctgcc ggtcatggcc accatgttct tccagtacga
    11221 ggagctgcac gaccgcgacc ggaccatgtg gggggagagc ccggccgacg tggcccggca
    11281 gctcctggcc gcgggcgcgg acatcgtcgg gatgaactgc gggcgcgggc cggatcgggc
    11341 catcgtgatc atccgcgaga tgcgccaggt cacggacgcg ccgctcatcg cgtacccgaa
    11401 tgcggggctg ccgatcacca ccggcgggcg gaccacctac gagctcggcc ccgaggagat
    11461 ggtcaaggac tacccggcac tcctcgatgc cggctgcaat atcgtcgggg cctgctgcgg
    11521 gtcgaacccc gagcacatcc gcctcatcgc cgacgtggtg cgctcgcgtc gcgcgcggtg
    11581 accgcggcgc tctccgtcct ctctcccacc cccgccgtcc tggaaggcct gccgctcgcg
    11641 atcgaccgcg acgaggtcct gcgcttccag ggctacaaga aggacgtgga cgtccccggc
    11701 ccccaggtgc tggcgatctt cgaggaagcc ctggccgagg gcctccgcct cctgacgccg
    11761 cgggtggtct accgcgcggt cgccgtgacc ggcggcgcgg cgggtcgtct cgacgcgggc
    11821 ggcgagcggc ttcacatccc gcagatcgcg cggctctggg gcgccgtcga ggcggtgggt
    11881 gcgggcattt gcacggtggg cgcgggcatc gaggcgcgcg tgcgcgagct cttcgacgcg
    11941 cgcgagttcc cgctggccgt gatgctcgac agcgtcgggt ccgccgcggt ggagggcctc
    12001 gccgagcacg ccaacgacct gctctgccag gccgggctcc gggccgggac gaaggtcacc
    12061 aaccggatca gcccgggcta cgcggggtgg gacaccgccg agcaggcggc gctcttccgc
    12121 ctgtgcccgg gcgaccccat cggggtttgc ctgaacgagg cgtgcttcat gacgccgggc
    12181 aagagcatca gctggctggt ggggatcggc cccggggcgc gggtggatca ctacttcacc
    12241 cagtgccggc gctgctggat gcgcgactgc gcctaccgcc gcgcccccgc cgacaccgcc
    12301 caccgaaatc agttagattc aagcgcgat
//
LOCUS       DASUFA010000215        13495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1292388, whole genome shotgun sequence.
ACCESSION   DASUFA010000215 DASUFA010000000
VERSION     DASUFA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13495
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292388"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFX87_07900"
     CDS             complement(<1..707)
                     /locus_tag="VFX87_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4994875.1"
                     /translation="MATARVLGASIKRREDPRFITGKGNYTDDLKLAGMTHAVFVRSP
                     HANAKIRKIDTAKASKAPGVVAIFTGKDMTGVNSLPCGWLLPELKIPPHMPLASDVAH
                     YVGDPVAIVIAESQSAASDAAEMVVVDWEVLPSVTSTEKAAAKGSPQIHEVAPNNEAF
                     KWQIGDAAATEAAFKAAPVVVKKRIINQRLVANAMEPRACVARYDDSTGDWTLWVTSQ
                     NPHVHRLLMTAFVLGIP"
     gene            complement(717..1172)
                     /locus_tag="VFX87_07905"
     CDS             complement(717..1172)
                     /locus_tag="VFX87_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4994876.1"
                     /translation="MTINGAPQSHEVEPRLLLVHYIREQAGLTGTHVGCDTSQCGACT
                     VHVNGVAVKSCTLLAVQCDGAQVLTIEGLAKDGKLHPIQDAFWEKHGLQCGFCTPGMI
                     MSAHQMLQRNPRPTEAQIRHDLEGNLCRCTGYHNIVKAIQYAAEKMAGK"
     gene            complement(1263..2450)
                     /gene="larC"
                     /locus_tag="VFX87_07910"
     CDS             complement(1263..2450)
                     /gene="larC"
                     /locus_tag="VFX87_07910"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX4994877.1"
                     /translation="MRLAYFDCPSGASGDMILGALVDAGAPFEALARELDGLGVHGYR
                     LERREVMKAGFRATQVDVALGHHEAGHRGLREILDILEGSRLAPAVRDMASRIFRRLA
                     EAEARVHGTTPERVHFHDVGAVDAIVDVTGACIGLHLLGVDAVHVGALPVGGGFVEGP
                     HGRMPVPGPATAELLKGFPTLDTGIRRELVTPTGAAILTTLAAGAGAMPAMRVTAVGY
                     GAGTMDLETPNVLRLFVGEAPTASVAAPAETIVQVETTVDDMSPQLYEPLLERLLEAG
                     ALDVWLTPVIMKRSRPGVVLTALCEPGRVADLSRLLFEESSTIGVRWTAYQRSRLERE
                     MVRLDTAYGPVTFKVSRLEGRVVTVTPEFEEVRRIARDRGLPVREVLEQARVEGRRLL
                     TTT"
     gene            complement(2514..3272)
                     /gene="larB"
                     /locus_tag="VFX87_07915"
     CDS             complement(2514..3272)
                     /gene="larB"
                     /locus_tag="VFX87_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX4994878.1"
                     /translation="MDREKVRALLEAVAAGAVAPEEALARLRALPVEDLRFARVDLHR
                     SLRHGVPEAVFCQGKTPEQVVAIVRRLAEAHDNVLATRAEPAVVAALLASGLPCRVHE
                     AARLVVANPVASEGVGLIVVVSAGTSDLPVAEEAAVTAEAMGNHVERVFDCGVAGLHR
                     LVPHLDRLNEANAIIAVAGMEGALPSVLGGLVDRPVIAVPTSVGYGASFGGVAALLAM
                     LNSCAPGVSVVNIDNGYGAAAQANQINKLASKLR"
     gene            complement(3254..4087)
                     /gene="larE"
                     /locus_tag="VFX87_07920"
     CDS             complement(3254..4087)
                     /gene="larE"
                     /locus_tag="VFX87_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX4994879.1"
                     /translation="MTDRDALDTKYDRLVDLLRGMTRVIVAFSGGVDSTFLARAAKDA
                     LGDGATLVTADSETYPASELEETRRLAGLLGMRQEIIRTRELDRPEYARNAPDRCFHC
                     KEELFARLEPIAQAAGTAQVIYGANMDDLGDHRPGMKAAEARGVRAPLIEAELWKEEI
                     RALSRELGLPTWDKPSFACLSSRFQYGDPITAEKLRRIDDAEAFVRSLGFRQFRVRHH
                     DRLARLELVPQEMARLWEEDRHGAIVRRFRELGYLYVTLDLQGFRSGSANEVLKWIGK
                     K"
     gene            complement(4084..5091)
                     /locus_tag="VFX87_07925"
     CDS             complement(4084..5091)
                     /locus_tag="VFX87_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX4994880.1"
                     /translation="MADRGELDVVGIGNALVDVLAHAEDGFHARQSLVKGTMHLVDEP
                     RAREIYDAMGPGVEISGGSAANTVVGVASFGGRAHYVGKVRDDQLGEVFGHDLRATGV
                     GYDTPRATEGPPTGRCLIVVTPDAQRTMSTFLGASVRLGPRDIDRGLITRGRILYLEG
                     YLFDPPEAQESFRTAAAIAHAAGRKVSLTLSDPFCVGRHRKAFLDLVEHHVDILFANE
                     AEICALYEVGDFDSAAARVRGHCEIAALTRSEKGSVILGDGRMHTVAPHPVAEVVDTT
                     GAGDLYAAGVLYGLSQGLDLPTCGRLGSLAAAEVIAHVGARPVVPLAQLAGPILGRGT
                     R"
     gene            complement(5084..5995)
                     /gene="mtnP"
                     /locus_tag="VFX87_07930"
     CDS             complement(5084..5995)
                     /gene="mtnP"
                     /locus_tag="VFX87_07930"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX4994881.1"
                     /translation="MSAARAKAGGEPALGIIGGSGLYEMEGIEDLRWVRVRTPFGEPS
                     DAYGTGRFGDRRVIFLPRHGRGHRFTPTEINYRANIYGLKALGVRWVISVSAVGSMKE
                     EIHPLDLVIPDQFYDHTSRRVSSFFGDGIVGHVGLADPVCPALGDLLENGSREAGARV
                     HRGGTYICIEGPQFSTKGESHIYRRWGVSVIGMTNMPEVKLAREAELCYATLALATDY
                     DVWHESHAAVTVEAVVANLLKNVATAKVVLKRVIPRVGKACGAGCGDALASAVITSPK
                     AFPPRTRKRLGLLLDRYFPPEGKARRG"
     gene            complement(5992..7317)
                     /gene="mtaB"
                     /locus_tag="VFX87_07935"
     CDS             complement(5992..7317)
                     /gene="mtaB"
                     /locus_tag="VFX87_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX4994882.1"
                     /translation="MSAPVTVALATLGCRLNQVESQEIGALLEGRGFRVVEPGAAAQV
                     YVINTCTVTGRADFSDRQLVRRIARENPEAYLVVTGCYAQTNPAAAAAIAGVDLVVGN
                     QEKYRLPELLGSLTKRPRPEVAVADIGTAGEIPIAPFKRVAGRSRAFVKIQDGCQHRC
                     AFCIVPAARGRSRSQEPKVVLDQVRALATAGYLDVTLTGVDIGHYGWDLHPRTTLAAL
                     LRELAEVDDLRWLRLSSVLPSYFTPELVDAVTTLPVMAPHLHLPLQSGSDRVLRLMRR
                     PYHTGMYRALVERLAAAIPGLGLGADVIVGHPGETEEDFEATMRFVEALPFSSLHVFA
                     YSDRKGTEAARRPDHVPAGVIREWSGRLRRLGALKSLAFRRGLVGRRREAVVLDARDR
                     RTGLLAGLTDNYVEVLFEGPDTLGRRVVPLTITEARADRTHGQLEEIGA"
     gene            complement(7314..8351)
                     /gene="rlmN"
                     /locus_tag="VFX87_07940"
     CDS             complement(7314..8351)
                     /gene="rlmN"
                     /locus_tag="VFX87_07940"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX4994883.1"
                     /translation="MSSTNLLDLSPAEMEELAQTLGAPRYRGRQLAQWIFVKGVTDLD
                     AMTDLPRDFRAALAAQTSVDLPEVERRTPSQDGSQKLVLRYGDGARVQAVLMPDADRL
                     TLCVSTQVGCGFGCAFCYTGTMGLERNLSAGEIVGQLMVAREGLAPGLRITHIVYMGM
                     GEPLANYTATVKSLRLLTDPQAFAFSPRRITVSTVGLVTGIERLAKENLRVNLAVSLH
                     ATSNEIRDRIMPVNRGFAIEELLAACRRFPLPFRQRMTFEYVLLEDVNDSVEDARRLV
                     KLLKGIRGKINLIPFNDWEGSSFARPPLARILDFQTVLLEHGIRATVRWSKGEDIGAA
                     CGQLREAAVPA"
     gene            complement(8607..10106)
                     /locus_tag="VFX87_07945"
     CDS             complement(8607..10106)
                     /locus_tag="VFX87_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994884.1"
                     /translation="MRLRGSLRLAAVSLWLLVGGPAGAQSAGELRVALPWTPENLDPT
                     MNLSSIRAKVGVGIFDSLVGRDAENRIAPQLAESWKALDDLTLQLKLRRGVVFHNGEP
                     FNAEAVRFTFERVLDPNQKSPNRANVGEVARVEVVDDYTVNLVLRQPYAPLLNRLIDF
                     PIVPPKYTVEKGNQGLALRPVGTGPFRFVELVKDDHLIVEAFDRHWRGAPRIRRIVYK
                     PIPEPFTRAAALRNNEVDVIDTVPPNLAGELERVGGIRVQRVPSTWIIYLGLNAFNKP
                     LSDVRVRQALNHATDVDALVKSVLDGNGRRMAGPFTPHMFGFNASVKGYGPDPARARR
                     LLAEAGYPDGVEITLEAPAGRYQGDKEIAEALGGQWQKAGFRPRVQVAEWGAYFKRYL
                     GKQFQDAYLLGLGGPMQDGDELYNLVSSKGRGLYYKNEKVDALFDAGRSTMDPAKRRK
                     IYADLARAMVADATWVFLMQQVDIYATRDRVTWTPRGDQWMLFHEAMLK"
     gene            complement(10118..11101)
                     /locus_tag="VFX87_07950"
     CDS             complement(10118..11101)
                     /locus_tag="VFX87_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX4994885.1"
                     /translation="MDKRERVLAALDRRPVDRPPVSFWRHVPDVDHTAPGLAEAMLAF
                     QRRWDLDFVKVMSSGVYCVEDWGCAVAYQGSPNGAKQCTTHAVQGRADWARIRALDPG
                     TGALGREIEAVRLIARGRADDAPILHTLFSPLTVARKLAGDRLVEDLRQAPEAVEPAL
                     DAITDTMARHARAALEAGADGLFLATQTAAPDVVTPEENARFDLPRMRRILAAVAGRS
                     AFTLLHVHGRDIFFDQAAALPVHGVNWHDRLTSPRLGDAHRRFKGALVGGLGESRTLL
                     TGPPDAVAAEVRDALAQTGGLGVIIGPGCVLPLATPDAHLAAVVAAVKAAA"
     gene            11205..11765
                     /locus_tag="VFX87_07955"
     CDS             11205..11765
                     /locus_tag="VFX87_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4994886.1"
                     /translation="MREAEAGDAPRFLDRLRAGDAPAFEELVMTYQHRVFGVALRMLG
                     NRAEAEDVAQEAFVRAHRALGAFRGDAKLSTWLYAITSRLCLNRLASGERRLTRQGED
                     AMLRLSDAGPRPDAALERRELETALGRAIAELPEARRIVVVLRDLEGLSYEEIAEVLE
                     LELGTVRSRLHRARADLKEKLERFLP"
     gene            11762..12844
                     /locus_tag="VFX87_07960"
     CDS             11762..12844
                     /locus_tag="VFX87_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX4994887.1"
                     /translation="MTCDETRDTLSAYLDGALAPDERSLVAAHLEGCAECRRELEALR
                     GTVALLKRVEAARAPAGFVDRVVAAARPRPWYRRAVDAVLLPLSVKLPLEATAVVMVG
                     LLAIYLFERSPELQRAAREVAPSQEAAGPAKEKAAEQLADRISRAPAPSSTPAPAEAP
                     ARAVGERDRADAPRSQEVNPQIPAAPPAGGAPTPAPVAPPAVSAPVAAPPPLPAAAPP
                     PEAKPQAKTEAAPSANVAGAARSEAESRERVLQPRAGGDSTRAAPSSARPAAKRVLPP
                     ADVVVRVAVKDRDAAERELAALIARLGGSVTQRRREDEATVVEAVVPQPRYAEFSDGV
                     ARIGSWQVEAECPDLPAQIHVILRLQ"
     gene            12914..>13495
                     /locus_tag="VFX87_07965"
     CDS             12914..>13495
                     /locus_tag="VFX87_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX4994888.1"
                     /translation="MRMLPKHLIAFTLAALLGAAPAAGAAPVKITAAEQRDVMVTVYN
                     GNLGLVKDVREVRLDAGLIETHFMDVAAQIDPTSVLLRSLSDPTGLRILEQNYEYDLL
                     SSEKLMEKYVGRKVRLYQGNGSFQEATLLSTRGPVYDINGQIHMGHFGNVVLPALPEN
                     LVSKPTLAWLLRNTRPAPQRVEASYLTAGISWKS"
BASE COUNT         1883 a         4879 c         4788 g         1945 t
ORIGIN      
        1 tcggggatgc ccagcacgaa cgcggtcatg agcagccggt gcacgtgggg gttctgggac
       61 gtgacccaga gcgtccagtc gccggtggag tcgtcgtagc gcgccacgca ggcccgcggc
      121 tccatggcat tggcgaccag gcgctggttg atgatccgct tcttcacgac gaccggcgcc
      181 gccttgaagg ccgcctcggt ggcggccgcg tcgccgatct gccacttgaa ggcctcgttg
      241 ttgggcgcca cctcgtggat ctggggcgag cccttggccg ccgccttctc ggtgctggtc
      301 accgagggca ggacctccca gtccaccacc accatctcgg ccgcgtcgct ggccgcggac
      361 tggctctccg cgatcacgat ggcgaccggg tcgcccacgt agtgcgccac gtcgctggcc
      421 agcggcatgt gcggcgggat cttcagctcc ggcaggagcc acccgcaggg cagcgagttg
      481 accccggtca tgtccttgcc ggtgaagatc gccacgactc ccggagcctt cgacgccttg
      541 gcggtgtcga tcttgcggat cttcgcgttg gcatgcgggc tgcgcacgaa caccgcgtgg
      601 gtcatcccgg ccagcttcag gtcgtcggtg tagttgccct tgccggtgat gaaccgggga
      661 tcctcacgcc gcttgatact cgcgccgagc acacgggcgg tggccatggt cgcgcctcac
      721 ttccccgcca tcttctcggc ggcgtactgg atggccttga cgatgttgtg atagccggtg
      781 cagcggcaca ggttgccttc gagatcgtgg cggatctgcg cctcggtcgg ccgggggttg
      841 cgctgcagca tctggtgggc ggacatgatc atgccgggcg tgcagaagcc gcattgcagg
      901 ccgtgcttct cccagaaggc gtcctggatc gggtggagct tgccgtcctt ggcgagcccc
      961 tcgatcgtga ggacctgggc gccgtcgcac tgcaccgcga gcagcgtgca cgacttcacg
     1021 gccacgccgt tgacgtgcac cgtacaggcg ccgcactggc tcgtgtcgca cccgacgtgc
     1081 gtgccggtca ggcccgcctg ttcccggatg tagtgcacga ggagcagccg cggctccact
     1141 tcgtgggact ggggggcacc gttgatcgtc atcgacacgg tctttttcac cgcgcaacct
     1201 cctgagtgaa gggtccgagg ccgggaaagc gcccccaacc gtatgccggg ccggggacgg
     1261 tgtcaagtgg tggtgaggag tcggcgaccc tcgacgcgtg cctgctccag aacttcccgg
     1321 accgggaggc ctcggtctcg cgcgatgcgg cgcacttcct cgaattccgg cgtgaccgtg
     1381 accacccggc cctcgagccg ggagaccttg aacgtcaccg gcccgtaggc ggtgtccagc
     1441 cgcaccatct cgcgctcgag gcgactgcgc tggtaggcgg tccagcgcac cccgatggtg
     1501 gaggattcct cgaagaggag ccgcgagaga tcggcgaccc gccccggctc gcagagggcg
     1561 gtcagcacga cccccggccg gctacgcttc atgatgacgg gcgtgagcca gacgtccagc
     1621 gcgccggcct cgaggagccg ctccagcagg ggctcgtaga gctggggcga catgtcgtcc
     1681 acggtggtct cgacctggac gatggtctcg gcgggcgcgg ccaccgaggc cgttggggcc
     1741 tcgcccacga agagccgcag cacgttgggc gtctcgagat ccatggtgcc ggccccgtac
     1801 ccgaccgcgg tgacccgcat tgccggcatc gcgcctgccc cggccgccag cgtcgtgagg
     1861 atggcggcgc cggtcggggt gaccagctcc cggcggatgc cggtgtcgag ggtcgggaag
     1921 cccttcagca gctcggcggt ggccgggccg ggcaccggca tgcggccgtg cgggccctcc
     1981 acgaagcccc cgcccaccgg cagcgcgccc acgtgcaccg cgtccacgcc caggaggtgg
     2041 aggccgatgc acgcgccggt gacgtccacg atggcgtcca ccgcgcccac gtcgtggaag
     2101 tgcacgcgct ccggcgtggt gccgtgcacc cgcgcctccg cctcggccag gcgccggaag
     2161 atgcgggaag ccatgtcccg caccgccggc gccagccggc ttccctccag aatgtcgagg
     2221 atctcccgga gcccgcggtg ccccgcctcg tgatggccga gcgccacgtc cacctgggtg
     2281 gcccggaagc ccgccttcat gacctcgcga cgctcgaggc ggtagccgtg cacgccgagc
     2341 ccgtccagct cgcgcgccag cgcctcgaac ggcgcgcccg cgtccaccag tgcgcccagg
     2401 atcatgtccc ccgaggcgcc gctcgggcaa tcgaagtagg cgaggcgcat cacgctacca
     2461 ccgtgacttc tctactgtat ccgctcggct cgccttcggc tgcgcctcgc ccatcaccgg
     2521 agcttggagg cgagcttgtt gatctggttg gcctgggccg cggcgccgta gccgttgtcg
     2581 atgttgacca ccgagacccc gggggcgcac gagttcagca tggccagcag cgcggccacg
     2641 cccccgaacg aggcgccgta gcccacgctg gtcggtaccg cgatcaccgg ccggtccacc
     2701 aggccgccga gcacgctggg gagggcgccc tccataccgg ccaccgcgat gatcgcgttc
     2761 gcctcgttga ggcggtccag atgcggcacc agccggtgga gccccgccac gccgcagtcg
     2821 aatacgcgct cgacatggtt gcccatcgcc tccgcggtga ccgcggcctc ctcggccacc
     2881 ggcagatcgg aggtgcccgc gctgacgacg acgatcagtc ccacgccctc gctcgccacc
     2941 ggattggcca cgaccagccg cgcggcctcg tgcacccggc acggcagccc gctcgcgagc
     3001 agcgcggcca ccaccgccgg ctcggcgcgc gtggcgagca cgttgtcgtg cgcctcggcg
     3061 agccgccgca cgatcgccac cacctgctcc ggggtcttgc cctggcagaa cacggcctcg
     3121 ggcacgccgt ggcgcagcga gcggtgcaga tccacgcggg cgaagcgcag atcctcgacg
     3181 ggcagcgcgc ggagccgggc cagcgcctcc tcgggcgcca ccgcgccggc cgcgaccgcc
     3241 tcgagcagcg cccttacttt ttcccgatcc acttcagcac ctcgttggcg ctgccactgc
     3301 ggaagccctg caggtcgagc gtcacgtaga gatagcccag ctcgcggaag cgccggacaa
     3361 tcgccccgtg ccggtcctcc tcccagaggc gcgccatctc ctgcgggacc agctccaggc
     3421 gggccaggcg gtcgtgatgg cgcacccgga actgccggaa gcccaggctg cgcacgaagg
     3481 cctcggcgtc gtcgatgcgc cggagcttct cggccgtgat ggggtcgccg tactggaagc
     3541 gggacgagag gcacgcgaag gacggcttgt cccaggtggg cagcccgagc tccctcgaga
     3601 gcgcccggat ctcctccttc cacagctcgg cctcgatcag cggggcgcgc acgccgcgcg
     3661 cttccgcggc cttcatgccg ggccggtgat cgcccaggtc gtccatgttg gcgccgtaga
     3721 tgacctgcgc ggttcccgcg gcctgcgcga tcggctcgag ccgcgcgaac agctcttcct
     3781 tgcagtggaa gcaccggtcg ggcgcgttgc gcgcgtactc cggccggtcg agctcgcggg
     3841 tgcggatgat ctcctggcgc atgccgagga ggccggccag ccgccgcgtc tcctcgagct
     3901 ccgaggccgg ataggtctcc gagtcggcgg tgaccagcgt cgcgccgtcg ccgagggcgt
     3961 ccttcgcggc ccgggcgaga aaggtcgagt cgacgccgcc ggagaaggcc acgatcacgc
     4021 gggtcatccc gcgcaggaga tccacgagcc gatcgtactt ggtgtcgagc gcgtcccgat
     4081 ccgtcatcgc gtgcccctcc ccaggatcgg tcccgcgagc tgcgccagcg gtaccaccgg
     4141 ccgggcgccc acgtgggcga tgacctcggc ggcggccagc gagccgaggc gcccgcaggt
     4201 cggcaggtcg aggccctggc tgagcccgta gagcaccccg gccgcgtaga gatcgcccgc
     4261 gcccgtcgtg tctaccacct cggccactgg atgcggggcg accgtgtgca tccgcccatc
     4321 ccccaggatc accgagccct tctcgctccg ggtgagcgcg gcgatctcgc agtgcccgcg
     4381 cacgcgggct gctgcgctgt cgaagtcgcc gacctcgtac agcgcgcaga tctccgcctc
     4441 gttggcgaag aggatgtcca cgtgatgctc gaccaggtcg aggaaggcct tgcgatggcg
     4501 ccccacgcag aaagggtccg acaaggtcag cgagaccttg cggccggcgg cgtgggcgat
     4561 ggccgcggcg gttcggaagg actcctgggc ctcgggcgga tcgaacagat agccctcgag
     4621 gtagaggatc ctgccgcggg tgatgagtcc ccggtcgatg tcgcgggggc cgagtcgcac
     4681 cgaggcgccc aggaacgtgc tcatggtgcg ctgggcgtcg ggcgtgacca cgatgaggca
     4741 gcgcccggtg ggcgggccct cggtggcgcg gggcgtgtcg tagcccaccc cggtggcgcg
     4801 cagatcgtgg ccgaacacct cgccgagctg gtcgtcgcgg accttgccga cgtagtgcgc
     4861 gcgcccgccg aaggaggcca cgccgaccac cgtgttggcg gccgagcccc ccgatatctc
     4921 cacgccgggc cccatcgcgt cgtagatctc gcgggcccgc ggctcgtcca ccaggtgcat
     4981 ggtccccttc accaggctct ggcgggcgtg gaacccgtcc tccgcgtggg ccagcacgtc
     5041 caccagcgcg ttgccgatgc cgacgacgtc gagctcgccc cgatcagcca cggcgcgcct
     5101 tgccctccgg cgggaagtag cgatcgagca gcaggcccaa gcgcttgcgg gtgcgcggcg
     5161 ggaacgcctt gggagaagtg atcaccgcgc tggcgagcgc gtcaccgcag ccggcgccgc
     5221 aggccttgcc cacccgcggg atgacgcgct tgagcacgac cttcgcggtg gccacgttct
     5281 tgagcaggtt ggccacgacc gcctccaccg tcaccgccgc gtggctctcg tgccacacgt
     5341 cgtagtcggt ggcgagcgcc agggtggcgt agcagagctc ggcctcgcgc gccagcttga
     5401 cctcgggcat gttcgtcatc ccgatgaccg atacgcccca gcgccggtag atgtgcgact
     5461 cgcccttggt ggagaactgc ggcccctcga tgcagatgta ggtgccgccg cggtgcaccc
     5521 gcgcgcccgc ttcccggctc ccgttctcga gcaggtcgcc cagcgccggg cagaccggat
     5581 ccgcgagccc cacgtggccg acgatgccgt cgccgaagaa gctcgagacg cggcggctgg
     5641 tgtgatcgta gaactggtcg gggatcacga ggtccagcgg gtggatctcc tccttcatgc
     5701 tgccgaccgc gctcaccgag atgacccagc ggacgcccag ggctttgagc ccatagatgt
     5761 tggcgcggta gttgatctcg gtgggcgtga accggtggcc gcggccgtgg cgcggcagga
     5821 agatcacgcg acgatcgccg aaccgtccgg tgccgtaggc atccgacggc tcgccgaacg
     5881 gcgtgcgcac gcgcacccag cgcagatcct cgatgccctc catctcgtag agcccgctgc
     5941 ctccgatgat gcccagcgcg ggctccccgc ccgccttggc cctggccgcg ctcatgcgcc
     6001 gatctcctcg agctgcccgt gggtgcggtc cgcgcgggcc tccgtgatcg tgagcggcac
     6061 gacccggcgg ccgagcgtgt cggggccctc gaagagcacc tcgacgtagt tgtcggtgag
     6121 gcccgcgagc agtccggtgc gccgatcgcg cgcgtcgagc accaccgcct cgcgccggcg
     6181 gccgaccagc ccgcggcgaa aggccaggct cttcagggcg cccagccggc gcagccgccc
     6241 gctccattcg cggatcaccc ccgccggcac gtggtcgggc cgccgcgccg cctcggtccc
     6301 cttgcggtcc gagtaggcga agacgtggag cgacgagaac ggcaacgcct cgacgaagcg
     6361 catcgtcgcc tcgaagtcct cctcggtctc gcccgggtgt cccacgatga cgtcggcgcc
     6421 caggcccagc ccgggaatcg ccgcggccag ccgctccacg agggcccggt acatcccggt
     6481 gtggtagggc cgccgcatca ggcgcagcac gcgatcgctg ccgctctgca gcggtaggtg
     6541 cagatgcggc gccatcaccg gcagcgtcgt caccgcgtcg acgagctccg gcgtgaagta
     6601 ggacggcagc accgaggaga ggcgcagcca ccggagatcg tccacctccg cgagctcccg
     6661 gagcagggcg gccagcgtcg tgcgcgggtg gagatcccag ccgtagtggc cgatgtccac
     6721 cccggtgagc gtgacgtcga gatagccggc ggtcgcgagg gcgcgcacct ggtcgagcac
     6781 gaccttgggc tcctggctcc ggctccgccc gcgcgcggcc ggcacgatgc agaaggcgca
     6841 gcggtgctgg cagccatcct ggatcttcac gaaggctcgt gagcggcccg ccacccgctt
     6901 gaagggcgcg atcgggatct cacccgcggt gccgatgtcc gccaccgcta cctcgggccg
     6961 cggccgcttc gtgagggagc cgagcagctc gggcaggcgg tacttctcct ggttgcccac
     7021 gaccaggtcc accccggcga tcgcggcggc cgccgccgga ttggtctggg cgtagcatcc
     7081 ggtcacgacc aggtaggcct cggggttctc gcgcgcgatg cggcggacga gctggcgatc
     7141 ggagaagtcc gcgcggcccg tcacggtgca ggtgttgatc acgtacacct gcgcggcggc
     7201 tccgggctcc accacccgga agccgcggcc ttccagcagc gcgccgatct cctgggactc
     7261 gacctgattg agccggcagc cgagggtggc cagcgccacc gtcaccggcg cgctcatgcc
     7321 gggaccgccg cctcgcggag ctggccgcag gcggcgccga tgtcctcgcc cttgctccac
     7381 cggacggtcg cccggatgcc gtgctcgagc agcacggtct ggaagtcgag gatccgcgcc
     7441 agcggcggcc gcgcgaagct cgagccctcc cagtcgttga acgggatcag gttgatcttg
     7501 ccgcggatgc ccttgagcag cttgaccagg cgccgcgcat cctcgaccga gtcgttgacg
     7561 tcctcgagca gcacgtactc gaacgtcatg cgctggcgga acggcagcgg gaagcgtcgg
     7621 caggccgcga gcagctcctc gatcgcgaag ccgcgattga ccggcatgat ccggtcgcgg
     7681 atctcgttgc tcgtggcgtg gagcgagacg gccaggttga cccggaggtt ctccttggcc
     7741 agccgctcga tgccggtcac cagccccacc gtggagaccg tgatgcgacg gggtgagaac
     7801 gcgaaggcct gcggatcggt caggaggcgg agcgacttca cggtggccgt gtagttggcg
     7861 agcggctcgc ccatgcccat gtagacgatg tgggtgatcc ggaggcccgg ggccagcccc
     7921 tcgcgggcca ccatcagctg gcccacgatc tctccggcgc tcaggttgcg ctcgagcccc
     7981 atcgtgccgg tgtagcagaa agcgcagccg aagccgcagc cgacctgggt ggacacgcag
     8041 agcgtgagcc gatcggcgtc cggcatcagc accgcctgga cacgtgcgcc gtctccgtag
     8101 cggagcacga gcttttggct cccgtcctgc gacggggtcc gccgctccac ctcgggcagg
     8161 tccacgctcg tctgcgcggc cagcgcggcg cggaagtccc ggggcaggtc ggtcatcgcg
     8221 tccagatcgg tcacgccctt gacgaagatc cactgggcaa gctggcggcc ccgatagcgc
     8281 ggggcgccca gggtctgggc cagctcctcc atctccgcgg gtgacagatc gagtagattc
     8341 gttgaggaca taagcaacgc tggatgataa cacatgggtg cgcgccctac ccgcggtcgc
     8401 ggccccagcg atgcccgcgc ctaatcctct ggagaacggc cggtggcctg cttcttgctc
     8461 ccgggggctc ttgcggaaat ccaccgaaac ggtgaatgcc tcgtggataa cccggtgggc
     8521 ttgtccgact ctcgcacgaa attccgcgca ctaggagcga gtcgccttct ttcgaggcac
     8581 ccggtcctga acggtcattc gcgtgcctac ttcagcatcg cctcgtggaa gagcatccac
     8641 tggtcgccgc gcggcgtcca cgtcacgcgg tcgcgcgtcg cgtagatgtc cacctgctgc
     8701 atgagaaaga cccacgtggc gtccgcgacc atcgcgcgcg ccagatcggc gtagatcttc
     8761 ctgcgcttcg cggggtccat ggtggagcgg cccgcgtcga agagcgcgtc caccttctcg
     8821 ttcttgtagt agaggccacg ccccttcgag gagaccaggt tgtagagctc gtcgccgtcc
     8881 tgcatcggcc cgccgagccc gagcaggtag gcgtcctgga actgcttgcc gagatagcgc
     8941 ttgaagtagg cgccccactc cgccacctgc acgcgcggcc tgaagcccgc cttctgccac
     9001 tggccgccca gcgcttccgc gatctccttg tcgccctggt agcggcccgc gggcgcctcc
     9061 agcgtgatct cgaccccgtc cgggtagccg gcttccgcga gcagacggcg ggcccgcgcg
     9121 ggatccgggc cgtagccctt gacggaggcg ttgaagccga acatgtgcgg cgtgaacggc
     9181 cccgccatgc ggcgcccgtt gccgtcgagc acgctcttca ccagcgcgtc cacgtcggtc
     9241 gcgtggttga gcgcctggcg cacgcgcacg tccgagaggg gcttgttgaa cgcgttcagc
     9301 ccgagataga tgatccacgt gctgggcacg cgctgcacgc ggatgccgcc gacccgctcg
     9361 agctcgccgg ccaggttggg cggcaccgtg tcgatcacgt ccacctcgtt gttgcggagg
     9421 gcggcggcgc gggtgaaggg ctcggggatg ggcttgtaga cgatgcgacg gatcctcgga
     9481 gcgccgcgcc agtgccggtc gaaggcctcg acgatcaggt gatcgtcctt gaccagctcc
     9541 acgaaccgga aggggccggt gcccaccggg cgaagcgcca gcccctggtt gcccttctcc
     9601 acggtgtact tcggcggcac gatcgggaag tcgatgagcc ggttcagcag cggcgcgtac
     9661 ggctggcgaa ggacgaggtt gaccgtgtag tcgtccacca cctccacccg cgcgacctcg
     9721 cccacgttcg cgcggttggg cgatttctgg ttgggatcga ggacgcgctc gaacgtgaaa
     9781 cgcactgcct ccgcgttgaa cggctcgccg ttgtggaaga cgaccccgcg gcgcagcttg
     9841 agctgcaggg tcaggtcgtc gagcgccttc cacgactcgg cgagctgcgg ggcgatgcga
     9901 ttctccgcgt cgcggcccac cagggagtcg aagatgccca cgcccacctt ggcccggatg
     9961 gacgagagat tcatcgtggg gtcgagattc tcgggcgtcc acggcagcgc gacccggagc
    10021 tcgccggccg actgggcacc cgcgggaccg cccacgagca gccacaggga caccgcggcc
    10081 agccggagcg acccgcgcag cctcatcggg ggcttcttca cgccgcggcc ttgaccgccg
    10141 ccaccaccgc ggccaggtgg gcgtccggcg tggccagcgg cagcacgcag cccggcccga
    10201 tgatgacccc gaggccgccg gtctgggcga gcgcatcgcg gacctccgcg gccaccgcgt
    10261 cgggcggccc ggtcaggagc gtccggctct cgccgagccc gcccacgagc gcgcccttga
    10321 agcggcggtg agcatcgccg agccggggcg aggtcaggcg atcgtgccag ttcaccccgt
    10381 gtaccggcag cgcggccgcc tggtcgaaga agatgtcgcg gccgtgcacg tgaagcagcg
    10441 tgaacgcgga gcgcccggcc accgcggcca ggatgcggcg catgcgcggc agatcgaagc
    10501 gcgcgttctc ctccggcgtc accacgtccg gcgcggcggt ctgggtggcg aggaagagcc
    10561 cgtccgctcc ggcctcgagc gcggcgcgcg cgtggcgcgc catggtgtcg gtgatggcgt
    10621 cgagcgcggg ctcgaccgcc tcgggcgcct gacggaggtc ctcgacgagc cgatcgcccg
    10681 ccagcttgcg ggccaccgtg agcggcgaga agagcgtgtg caggatcggg gcgtcgtcgg
    10741 cgcggccgcg cgcgatcagg cgcaccgcct cgatctcccg gccgagggcc ccggtccccg
    10801 ggtcgagcgc gcggatccgc gcccagtcgg cccggccctg caccgcgtgc gtcgtgcact
    10861 gcttggcccc gttgggtgag ccctggtagg cgaccgcgca cccccagtcc tccacgcagt
    10921 agacgccgct cgacatcacc ttgacgaagt cgaggtccca acgccgctgg aaggccagca
    10981 tggcctcggc gaggccgggg gccgtgtggt ccacgtcggg cacgtggcgc cagaaggaca
    11041 ccggcgggcg atccaccggc ctgcggtcga gggcggccag cacgcgctcc cgcttgtcca
    11101 tgatgccgcg gagtctacca cgcgggtcga gagccggtcc cccgccctgc cccgcccgtg
    11161 ggcttttgtg cgcgggaact tccgcgccgc ggtatggtct taccgtgcga gaggcggagg
    11221 caggcgacgc gccgcgcttc ctggaccggc ttcgcgccgg cgatgccccc gccttcgagg
    11281 agctggtgat gacgtaccag catcgcgtgt tcggggtggc ccttcgcatg ctgggcaacc
    11341 gggccgaggc ggaggacgtg gcgcaggagg ccttcgtccg cgcccatcgc gcgctcggcg
    11401 cgtttcgcgg cgacgccaag ctctcgacct ggctctacgc catcacctcg cgcctgtgcc
    11461 tgaaccggct cgcctccggg gagcggcgtc tgacgcggca gggggaggac gcgatgctgc
    11521 ggctgtccga cgcgggcccg cgcccggatg cggcgctgga gcgtcgcgag ctcgagaccg
    11581 cgctcgggcg ggccatcgcc gagctgccgg aggcccggcg catcgtggtg gtgctccgcg
    11641 acctcgaagg cctctcctac gaggagatcg cggaggtgct ggagctggag ctcgggacgg
    11701 ttcgatcccg tctgcaccgg gcccgggcgg atctgaagga gaagctggag cgattcctgc
    11761 catgacctgc gacgagacgc gcgacacgct gtcggcctat ctggacgggg ccctcgctcc
    11821 ggacgagcgg agcctggtcg ctgcgcatct ggagggctgt gccgagtgcc ggcgcgagct
    11881 cgaggccctg cgtggaacgg tcgcgctgct caagcgggtg gaggccgccc gggcgcccgc
    11941 gggcttcgtg gaccgggtgg tcgccgcggc gcggccgcgg ccctggtacc gtcgcgcggt
    12001 ggacgcggtg ctcctgccgc tctcggtcaa gctcccgctc gaggccaccg cggtggtgat
    12061 ggtgggactg ctcgcgatct acctcttcga gcgctcgccg gagctgcagc gggccgcgcg
    12121 cgaggtagcc ccgagccagg aggccgcggg gcccgcgaag gagaaggcgg cggagcagct
    12181 cgcggaccgg atctcgcggg cgccggctcc atcatccacg ccggcgcccg ccgaggcgcc
    12241 ggcgcgagcg gtcggcgagc gtgaccgcgc tgatgcgccc cgatcgcagg aggtcaaccc
    12301 gcagatcccg gccgccccgc ccgccggggg cgcccccacg cccgcccccg tggcgccccc
    12361 cgccgtctcc gcgccggtgg cggcgccccc gcctctgccg gcggccgcgc cgccgcccga
    12421 ggccaagccg caggcgaaga cggaggccgc gccgagcgcg aacgtggccg gcgccgcccg
    12481 ctccgaggcc gagagccgcg agcgggtgct ccagcccagg gccggcggag actcgacgcg
    12541 tgccgctccg tcatcggcgc ggccggccgc caagcgggtg ttgcccccgg cggacgtggt
    12601 ggtccgggtg gccgtcaagg atcgcgacgc ggcggagcgc gagctcgccg cgctcatcgc
    12661 gcgcctgggc ggcagcgtca cgcagcgccg gcgcgaggac gaggccaccg tggtggaggc
    12721 cgtggttccg cagccgcgct acgcggagtt ctccgacggc gtggcacgca tcggctcctg
    12781 gcaggtcgag gccgagtgcc cggatctccc cgcccagatt cacgtcatcc tccgcctgca
    12841 gtagcccgga acttctccgg gatccccggg tctaaccgga cagcaacagt catccggttc
    12901 ctggaggacc acgatgagaa tgcttccgaa acacctgatc gcattcaccc tggccgcctt
    12961 gctcggcgcg gcccccgccg ccggcgcggc cccggtcaag atcaccgccg ccgagcagcg
    13021 cgacgtcatg gtcacggtct acaacggcaa tctcggcctc gtgaaggatg tgcgcgaggt
    13081 ccggctcgac gccggcctga tcgagaccca cttcatggac gtggccgccc agatcgatcc
    13141 cacctccgtg ctgctgcgct cactcagtga cccgaccgga ctcaggatcc tcgagcaaaa
    13201 ctacgagtac gacctgctct cgagcgagaa gctcatggag aagtacgtgg gccggaaggt
    13261 gcggctctac cagggcaacg gcagcttcca ggaggccacg cttctctcca cgcggggccc
    13321 cgtctacgac atcaacggcc agatccacat ggggcacttc ggcaacgtgg tgctgcccgc
    13381 cctgcccgag aacctggtgt cgaagccgac gctggcctgg ctgctgcgga acacgcgccc
    13441 ggcgccacag cgcgtggagg cctcctatct caccgccggc atcagctgga agtcc
//
LOCUS       DASUFA010000216         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1423140, whole genome shotgun sequence.
ACCESSION   DASUFA010000216 DASUFA010000000
VERSION     DASUFA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423140"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="hpnH"
                     /locus_tag="VFX87_07970"
     CDS             <1..712
                     /gene="hpnH"
                     /locus_tag="VFX87_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX4994889.1"
                     /translation="VYLCTNAILLKEKLDLFTPSKYLSFSVHMDGLREEHDEAVCRDG
                     VYDQAVPAIREAVARGFRVTTNSTLFDGASPLRMREFFDEMMNLGVEGMMISPGYSYS
                     KAPDQEHFLLQSRTHELFTRMLSKPKKRWRFNQSPLFLRFLMGKHDFECTPWGNPTYN
                     MFGWQRPCYLLQEGYTKTFRELMDTTPWENYGKKSGNEKCRDCMVHCGYEATAVDYTF
                     SSMKGFVETVGVTLTGKL"
     gene            723..1082
                     /locus_tag="VFX87_07975"
     CDS             723..1082
                     /locus_tag="VFX87_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994890.1"
                     /translation="MTLEGWVPEVGQYLTLAEVVEFAFDYRGNTTLVKTDGAEVVGYI
                     FNRDTYGPEPFLEYFDESGEGPFTLRYSEVANIKFSGKDTAAGNSWRAWLERKEREKA
                     EKATAAGSVRDPDPHRG"
     gene            1051..1767
                     /locus_tag="VFX87_07980"
     CDS             1051..1767
                     /locus_tag="VFX87_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994891.1"
                     /translation="MSGILILTAVELEARVLARRLELPPITVLPFPAFGRGLIRVAPV
                     GLRAGLLASRWPRLLAGFNNPLVVSGGVCGGLAPDLSAGDLVLPESVLGPSGERLNVT
                     PTPHRQATELAGSARGGTLVTSREVVATAEAKAALFASSGAVAVDMESAVILAHAAAA
                     GCPTLVVRGVSDAASESVPFELIDLMTPAGKLRAVRALALTVTRPRLLPRALALRQAT
                     RRALAAVARVLAALPASSRP"
     gene            1764..2774
                     /gene="hpnA"
                     /locus_tag="VFX87_07985"
     CDS             1764..2774
                     /gene="hpnA"
                     /locus_tag="VFX87_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEX4994892.1"
                     /translation="MNVLVTGGTGFVGGNLVRELLADGHTVRVLARPGAGRLALEGCA
                     VDIVEGDLLDARSLRVAVAGARHVYHTAADYRLWAPDLQVLYRINVDGTRHLLAAAAE
                     AGAERIIYTSTVGAVGIPKNGIPGDETTPVSLADMVGAYKASKFLAERVADEWVARGA
                     PVVIVNPSAPIGPWDVRPTPTGQMIVDFLNGKMVGSVDTGLNVVHVRDVARGHILAAE
                     KGRVGERYILGHRNLSLIEIFEMLSTLTGVPAPRFRVPYAVAWMAAAAMEGVSRITRR
                     PPAVSLTAVRMSRKRMYFSAEKAIRELGLPQTPAETALKDAVAWFTAHGYANAKRNGR
                     SA"
     gene            2787..3635
                     /gene="hpnD"
                     /locus_tag="VFX87_07990"
     CDS             2787..3635
                     /gene="hpnD"
                     /locus_tag="VFX87_07990"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX4994893.1"
                     /translation="MNAGQFVSRLTRKSRSNFFYAFLCLPRAQREAIYACYAFCRIVD
                     DAVDLVSDRAQQRAELGRWREEIARVYEGRPEHPAGQRLQVAVRRFPIPRAALEEIIA
                     GVEMDMDHPTYETFEALYPYCYRVASAVGLCCIEIFGYSDPRAREYAVNLGVALQITN
                     ILRDVQVDARIGRVYLPQADLRRFGVTAEDLAQGRYTPGFVQLMTFEAARAHEYYRRA
                     WAALPAADARRLFAAEIMGRTYFALLRAIEARRFDVFAGRVTLSTPRRLAIAFRCWLG
                     ARLATS"
     gene            3632..>4030
                     /locus_tag="VFX87_07995"
     CDS             3632..>4030
                     /locus_tag="VFX87_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4994894.1"
                     /translation="MKAAVCIAGAIELREWPAPAPGPGELLLRVSGCGLCGSDILKIS
                     VAAPEPAVLGHEVVGRVVEVGPGVRRFAPGDRLVVAHHVPCFRCHYCRRGSPSMCRHF
                     KRVNLDPGGFAELVRVPAPNVEHAAFRLPAE"
BASE COUNT          552 a         1450 c         1407 g          621 t
ORIGIN      
        1 cgtgtatctg tgcacgaacg cgatcctgct gaaggagaag ctcgacctgt tcaccccgtc
       61 gaagtacctc tcgttcagcg tccacatgga cggcctccgc gaggagcacg acgaggcggt
      121 atgccgcgac ggcgtctacg atcaggcggt gcccgcgatc cgagaggcgg tggcgcgcgg
      181 cttccgggtg accaccaaca gcacgctctt cgacggggcc tcgccgctcc ggatgcgcga
      241 gttcttcgac gagatgatga acctgggcgt cgagggcatg atgatctccc cgggctacag
      301 ttactccaag gcgcccgacc aggagcactt cctgctgcag tcgcgcaccc acgagctgtt
      361 caccaggatg ctctcgaagc ccaagaagcg ctggcggttc aaccagtcgc cgctcttcct
      421 ccgcttcctg atgggcaagc acgacttcga gtgcaccccc tggggcaacc cgacctacaa
      481 catgttcggg tggcagcgcc cgtgctacct gctgcaagag gggtacacga agaccttccg
      541 ggagctgatg gacaccaccc cctgggagaa ctacggcaag aagagcggca acgagaagtg
      601 tcgcgactgc atggtgcact gcgggtacga ggccactgcc gtggactaca cgttcagctc
      661 gatgaagggc ttcgtggaga cggtcggggt caccctgacc ggcaagctgt aggagcagcc
      721 gcatgaccct cgaaggctgg gtgcccgagg tcggtcagta cctcacgctg gcggaagtgg
      781 tggagttcgc gttcgactac cggggcaaca ccaccctcgt caagaccgac ggcgccgagg
      841 tggtcggcta tatcttcaac cgtgatacgt atgggcccga gcccttcctc gagtatttcg
      901 acgagagcgg cgaggggccc ttcacccttc gctactccga ggtcgccaac atcaagttct
      961 ccggcaagga caccgcggcc gggaatagct ggcgggcgtg gctcgagcgc aaggagcggg
     1021 agaaggccga gaaggccacg gccgccggga gtgtccggga tcctgatcct caccgcggtt
     1081 gagctcgagg cgcgggtcct ggcccgccgg ctcgagcttc cgcccatcac cgttctgccc
     1141 tttcccgcat tcggtcgcgg cctgatcagg gtggccccgg ttggcctgcg ggccggtctc
     1201 ctcgcctcgc gctggccacg gcttctcgcc ggcttcaaca atccgctcgt ggtctcgggt
     1261 ggggtctgcg ggggcctcgc gcccgatctg agcgcgggcg acctcgtcct gcccgagagc
     1321 gtgctcgggc ccagcgggga gcggctcaac gtgacgccca cgccgcaccg gcaggccacc
     1381 gagctggccg gctccgcgcg cggcggcacg ctcgtgacct cccgcgaggt ggtggcgacg
     1441 gccgaggcca aggcggcgct cttcgcgagc agcggcgcgg tggcggtgga catggagtcc
     1501 gcggtcatcc tggcgcacgc ggcggccgcg ggctgcccca cgctggtcgt gcgcggcgtg
     1561 tcggacgcgg cgagcgagag cgtgccgttc gagctcatcg acctgatgac gccggcgggg
     1621 aagctgcgtg ccgtgcgcgc gctcgcgctg acggtcacgc ggccgcggct cctgccgcgc
     1681 gccctggccc tgcgccaggc cacccggcgg gccctcgccg cggtcgcccg ggtgctcgcg
     1741 gcgctccccg cctcctcccg gccgtgaacg tcctcgtcac cggcggcacc ggcttcgtgg
     1801 ggggcaacct cgtgcgcgag ctcctcgcgg acggccacac ggtgcgggtg ctggcgcggc
     1861 ccggggccgg ccgcctcgct ctcgagggct gcgcggtgga catcgtcgag ggcgatctgc
     1921 tggacgcccg ctcgctccgc gtcgcggtgg ccggggcccg tcacgtctac cacacggccg
     1981 ccgactaccg gctctgggcg ccggacctcc aggtcctcta ccgtatcaac gtggacggca
     2041 cgcggcacct gctggccgcg gccgccgagg cgggcgccga gcgcatcatc tatacgtcca
     2101 cggtgggcgc ggtcggcatc cccaagaacg gcatcccggg cgatgagacg acgccggtgt
     2161 cgctggccga catggtcggc gcctacaagg cctccaagtt cctggccgag cgcgtggccg
     2221 acgaatgggt ggcgcgcgga gcgccggtcg tcatcgtcaa cccgtcggcg cccatcggcc
     2281 cgtgggacgt caggcccacc ccgaccgggc agatgatcgt ggacttcctg aacggcaaga
     2341 tggtcggctc ggtggacacc ggcctcaacg tggtccacgt gcgtgacgtg gcgcgcggcc
     2401 acatcctggc ggccgagaag ggccgggtgg gggagcgcta catcctgggc caccgcaacc
     2461 tctccctgat cgagatcttc gagatgctct cgacgctgac cggcgtgccc gcgccgcgct
     2521 tccgcgtgcc ctacgcggtg gcgtggatgg cggcggcggc gatggaaggc gtctcccgca
     2581 tcacccgccg cccccccgcg gtctccctca ccgcggtccg catgtcccgc aagcgcatgt
     2641 acttcagcgc cgagaaggca attcgagaac tggggctgcc ccagactccg gccgagaccg
     2701 ccctcaagga cgcggtcgct tggtttaccg cccacggtta cgcgaacgcc aaacggaatg
     2761 gccggagcgc gtgacgtggg gacggcatga acgcaggcca gttcgtctcg cgcctcaccc
     2821 ggaagagccg ctcgaacttc ttctacgcgt tcctctgcct gccgcgggcc cagcgcgagg
     2881 ccatctacgc gtgctacgcc ttctgccgca tcgtggacga cgcggtggac ctggtgtcgg
     2941 atcgcgcgca gcagcgcgcc gagctcgggc gctggcgcga ggagatcgcg cgcgtctacg
     3001 aggggcggcc tgagcatccg gccggccagc ggctgcaggt ggcggtccgc cgcttcccga
     3061 tcccgcgcgc cgcgctcgag gagatcatcg cgggagtgga gatggacatg gaccatccca
     3121 cctacgagac cttcgaggcg ctctatccct actgctatcg cgtggcctcc gcggtggggc
     3181 tctgctgcat cgagatcttc ggctactcgg acccgcgcgc gcgcgagtac gcggtgaacc
     3241 tgggcgtcgc gctccagatc accaatatcc tccgggacgt ccaggtggac gcgcgcatcg
     3301 ggcgcgtcta cctcccgcag gcggacctgc ggcgcttcgg cgtgaccgcg gaggatttgg
     3361 cgcagggccg ctatactccc gggttcgtcc agctcatgac cttcgaggcg gcccgcgccc
     3421 acgagtacta ccggcgggcg tgggccgcgc tgcccgcggc ggatgcgcgc cggctcttcg
     3481 ccgccgagat catgggccgc acctacttcg cgctgctgcg ggccatcgag gcgcgccgct
     3541 tcgacgtctt cgccggtcgc gtgaccctct cgacgccccg ccgcctcgcc atcgccttcc
     3601 gctgctggct cggcgcccgg ctggccacct cgtgaaagcc gccgtctgca tcgcgggtgc
     3661 catcgagctg cgcgagtggc ccgcgccggc cccgggcccc ggcgagctcc tgctgcgcgt
     3721 gagcggctgc gggctctgcg gctccgacat cctcaagatc agtgtggccg cgcccgagcc
     3781 cgccgtcctc ggccacgagg tggtggggcg ggtcgtggaa gtcgggcccg gcgtgcgacg
     3841 cttcgcgccc ggcgaccggc tcgtggtcgc ccatcacgtg ccgtgcttcc gctgccacta
     3901 ctgccggcgc ggcagcccgt ccatgtgccg ccacttcaag cgggtcaatc tcgatccggg
     3961 cggattcgcg gagctggtgc gcgtgcccgc gcccaacgtg gagcacgccg ccttccggct
     4021 tcccgccgag
//
LOCUS       DASUFA010000217        11042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1177737, whole genome shotgun sequence.
ACCESSION   DASUFA010000217 DASUFA010000000
VERSION     DASUFA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11042
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177737"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFX87_08000"
     CDS             complement(<1..340)
                     /locus_tag="VFX87_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX4994895.1"
                     /translation="MESAFELNAGRLCLDFTNTVRARPLSEKVDLIRSYEDLLSWARQ
                     ATILTPGETAALGETARQQTRAATDALAQALALREALYGIFSARAAGLPAPASDLHTV
                     NKAIGKAMTRA"
     gene            complement(393..2645)
                     /locus_tag="VFX87_08005"
     CDS             complement(393..2645)
                     /locus_tag="VFX87_08005"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX4994896.1"
                     /translation="MFAAGIAVGLILSVVAIVMALVALSAARARRPAGTDERVAALEE
                     RVRGLVYRVWRLEGGPAAGTPLESPAAPDVAPVVETAPAPPPPGPPVEGAPRLEAPSA
                     APPMPPHRGLDLEQRIGARWATWVGIVAILVGVALFLKWAFDNAFLGPAARVGLGVVA
                     GLVMLGGGLLLHRRRDVPYLSEGLAGGGLGVLYLSLFAAHALYGLVGPTPAFVAMFAV
                     TLLGTAVAVASSRLTTAVLAVLGGLLTPVLLQVERPDERNLLAYLLVLDALVLLVARF
                     RTWPSLNRLAWLGSTLLLLPTLLHEPEAPRPLTRLLLLSALFLIFLAVPLFRERVEGR
                     RADRIDLALVVANAAAYFWAVYVTLEAWRPSWEGAYALGLAVLYRLVAADYASRVPDD
                     RVTVDLHEGVSWTFLTIAIPVALDGQWVTLAWAVQGVVLLWLASRSPAPVAAWGGLAV
                     LLLAAVRAVALDAYWSADTTPVWNLAFLVHLLVVVALAAGGGLGGRALGLVGAGWTPA
                     NLRATLWVAAVGTLAVQLWREPSGHWPAVLLMAELVVVGLLARASSSLAFVVATPLLA
                     GVVLVRVLGVDDDLARRAAGSLVSLAFLIRVAACVAISLAGGALARSTATPRAAPLGQ
                     VLSGVAGLALLFVLSVNWTRYQESRVSAARAAGRNQLLGELRWRTQVGLSLLWTLYAA
                     AALAWGFLRSNPAVRYAALGLFGLTVFKVFAVDLGAVKTAYRILSFLVLGVVLLLVSL
                     AYQKRRRQSP"
     gene            2734..3309
                     /locus_tag="VFX87_08010"
     CDS             2734..3309
                     /locus_tag="VFX87_08010"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX4994897.1"
                     /translation="MLRPSRAGRWAVAALVLAALVLPHAVEAEPLRFRIQPEASEISF
                     RATSRLMNADGRFSRFSGDVVVDPAALTTARITLTIDAASLDTGIEMRDNHLRSSDFF
                     DVARFPTVSFQSVRIEAAGRRAIVVGRLTLHGVTREIAVPIDVQITNIALVASGEFIV
                     NRGEYAMNYNSFVNPIGNEVRVAFTFRARIP"
     gene            3348..4022
                     /locus_tag="VFX87_08015"
     CDS             3348..4022
                     /locus_tag="VFX87_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEX4994898.1"
                     /translation="MTELLDLTSRPVAVTFTATAPAGVPRVAKAGPAGCAYWKQAAEG
                     AVFYTEAADHYNCPVGSFTHGIALPAERARELEDVVGTMVGLQYIRMEEVGALPRRPA
                     PFGLAVYAPLAETPAPPDVVLVRGRAREIMLVAEAARAAGIAGDGAAMGRPACAMIPA
                     VLGGPAGVTSLGCIGNRVYTGLGDDELYFTIPGSKIGEVVERLETVVDANRALQTYHE
                     GRRATV"
     gene            4090..5283
                     /locus_tag="VFX87_08020"
     CDS             4090..5283
                     /locus_tag="VFX87_08020"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994899.1"
                     /translation="MNAPLAGLLVADLTQNVAGPYCTQILGDLGAEIVKVERLGRGDD
                     ARAWGPPFWGQESATFMSVNRNKRSLAVDLKADEGRAVVERLLGRADVFVQSLRAGAA
                     EELGLGYARVAALNPRLVYCSVTAFGTEGPLKDRPGYDPLMQAYGGIMSINGHPNQPP
                     ARVPVSVVDMGTGMWAVMGILAALRERDRTGHGAEVTTALFDTALAWSVFQMSHYFAT
                     GEVPRPQGSGTAMIVPYEAFPVVDGWVMIAAGSDALFVKATTALEVPELARDPRFVDN
                     PRRVEHRGALQEALARVTRVMTAEQLLGRLQRAGVPSARVSTMDQVAAEPQTEASGML
                     PAVKHPRLPDYRAVALPIKWDGERPAASRVPPLLGEHSAEVLAELGYDDAAIRGLVER
                     HVIQL"
     gene            5267..6061
                     /locus_tag="VFX87_08025"
     CDS             5267..6061
                     /locus_tag="VFX87_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4994900.1"
                     /translation="MSSSYRTLNVATSADGHVVTVELNRPEALNAMNTAMGEDLLRCF
                     EDLHRDRTVRAVVFTGAGTKAFCVGGDLKEREGMTDETWRAQHVIFEAAAARVLHCPV
                     PVIAAVEGFAMGGGCELAVLSDFVVCGETAVFAVPEVTRGIFPGIGGTQLLPRILGAP
                     MAKEMIFTGRRVDAREAKALGLVNHVVPAGQARAKALEIAATIADNGPIAVRQAKKAV
                     NRGGETDLETGMILAIEAYNNTVTTDDRLEGVRAFNEKRKPRFTGK"
     gene            6095..7258
                     /locus_tag="VFX87_08030"
     CDS             6095..7258
                     /locus_tag="VFX87_08030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4994901.1"
                     /translation="MTFVLSDEQKALKAAVYDLCKQYPAEYWRDLDSRREYPEAFVSE
                     LTKAGYLAVLIPEEYGGGGLGIMEAALILEEIGRAGGNPAACHAQMYIMGTVLRHGTD
                     AQKREYLPKIAAGELRLQAFGVTEPNAGSDTTKLQTTAVRKGDRYVVNGQKMFISRVL
                     QSDLMLLLARTTPVDQVKKKTDGLSVFLIDIRELKGKGLDVRPLRMMMNHSTNALFFD
                     NMEIPATSLIGQEGRGFSYILDGMNAERILVASDSLGDARWFTEKAVAYAGQRVIFGK
                     PIGANQGVQFPIAKAHVSIEAADLMRTKAARMFDAGIPCGPEANMAKYLASEAAIEAG
                     NACIDCHGGYGFAEEYDIERKFRESRLYRAAPINNNLVIAYVGEHVLGMPRSY"
     gene            7333..7887
                     /locus_tag="VFX87_08035"
     CDS             7333..7887
                     /locus_tag="VFX87_08035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX4994902.1"
                     /translation="MLGPILEGERVRLEPPRPEYFPAYQRWFADMEVTRYLLYRFPFT
                     TRWEEEWLEQAGRDPAQVIWAIVLKHNGRLIGNAALEHIDWRDRRAESGIVIGEKDEW
                     GKGYAGEAMRLRTRYAFRELGLETVTTRVVLTNAASRRGLERAGYRQAGVLRHHYFVD
                     GRWHDAWVGEILKDEWEATQETTQ"
     gene            7890..8834
                     /locus_tag="VFX87_08040"
     CDS             7890..8834
                     /locus_tag="VFX87_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX4994903.1"
                     /translation="MPRHVTICEVGTRDGFQIEPDFIPTDQKIDTVNRLSGAGIPRIE
                     VTSFVSPKAIPQLRDAAEVMAAITRRPGTTYSALVPNDKGAVRAVDARVDEIHTVVSA
                     SESHNLANVKMTIAESLTKLGAVSEVARRASVPVVSGISCSFGCPFEGDVPVAHLESV
                     VARLVDMGARGIGLADTTGMANPVQVGRVLERLVPRFPGVEWTLHTHDTRAMAIPNIL
                     TAMEYGVTHFDASIGGLGGCPFAPGASGNVCTEDLIHCLHAMGVETGVDLDALIETSK
                     RVQAIVGRELPGQLVKAGKWDRRYPLPDGVRERIPARS"
     gene            8831..9388
                     /locus_tag="VFX87_08045"
     CDS             8831..9388
                     /locus_tag="VFX87_08045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4994904.1"
                     /translation="MTKTATRMLRLMDRAVTHWRIPVIGREKGRVLRRLLLKHQPRRG
                     IEVGSLFGYSAILVAGHLPRGGRLTCVEQNPYLAKFVASNVEEAGLAKRVKVVVGDAL
                     RVLPLLRGPVDFLLLDAKKEDYLDYLRAVEPRLRKGALVVADNTGIYRRDVKPYLDHV
                     RASGRYESREHDFGSDCMEVSVFRG"
     gene            9395..9898
                     /locus_tag="VFX87_08050"
     CDS             9395..9898
                     /locus_tag="VFX87_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994905.1"
                     /translation="MLGEQHDPEFLKHLFETTEILRKPVAGIIAGYHVLPYILVGPQE
                     TKPASSVEIRGKIKVSPRLVLAPGRAGPTYGDLFKENELMDEALIARVFSFAYSNRHQ
                     VTLESEDLKIQRWDRNPQAQVERALDELSQREVINTGVILAPNVRFYPVSLDRFITEI
                     LDQEFKS"
     gene            9895..10689
                     /locus_tag="VFX87_08055"
     CDS             9895..10689
                     /locus_tag="VFX87_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEX4994906.1"
                     /translation="MTTETYDWDAIVNGLNQYLRLRTIPIGMKLFETVEEMEAIPKIR
                     RPKSKHTTDQIVAQARQLGWTVGITMADLIGAQCGAVIGLHPQDAEWLSGKRMAGVWF
                     KTQEDASLHQHAMDCVPHGRYRAMAVAPLTSGRLDPPDICLIYGTPGQMIFVINGLQW
                     IGYKKMSFTSVGESACADSWGKALKTGEPALTIPCYAERRYGGVADDELLMAIPPRFL
                     PKVVEGLAALSKNGLRYPIPPYGIQSDAGAGLAVSYPGAPAGSAGR"
     gene            complement(10694..>11042)
                     /locus_tag="VFX87_08060"
     CDS             complement(10694..>11042)
                     /locus_tag="VFX87_08060"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX4994907.1"
                     /translation="TMLALAGGTPGPQVQGRSLVPLLGGRRPPDWRTSFLVEYWSENA
                     MPWLVGMTYKAVRTERAKLIHWVNRDGVDELYDLAADPYELRNLHDEPARASERDALR
                     RELARLVTEAVGL"
BASE COUNT         1759 a         3927 c         3917 g         1439 t
ORIGIN      
        1 ccgcccgggt catcgccttg ccaattgcct tgttgacggt gtgaagatcg gaggccgggg
       61 cgggcaggcc ggcggctcgc gccgagaaga tcccgtagag cgcttcccgg agcgcgaggg
      121 cctgggccag cgcatccgtg gccgcgcggg tctgctggcg ggccgtctcg cccagcgcag
      181 ccgtctcccc gggggtcagg atggtggcct ggcgggccca ggagagcaag tcttcgtagc
      241 tgcggatcag atcgaccttc tcgctgagcg ggcgggcccg gaccgtgttg gtgaaatcga
      301 ggcacagacg gcccgcgttg agctcgaacg cgctttccat cgagccgact atataacctt
      361 caaactgaat tttgaaagtt ttgtcatctt gtctaggggc tttgcctgcg tcgtttctgg
      421 tacgccaggc tgaccaggag caggaccacg cccaggacga ggaacgacag gattcggtag
      481 gcggtcttga ccgcaccgag atcgacggca aacaccttga agacggttag accgaagagc
      541 cccaacgcgg cgtaccgcac cgccgggttc gagcggagga agccccaggc cagggcggcg
      601 gccgcgtaga gcgtccagag cagcgagagc ccgacctggg tccgccaccg cagctccccg
      661 aggagctggt tccgcccggc ggcgcgggcc gccgacacgc ggctctcctg gtatcgcgtc
      721 cagttcacgc tcaggacgaa gaggagggcc aggccggcca cccccgagag gacctggccc
      781 agcggggcgg cccggggtgt ggccgtggag cgggccaggg ccccaccggc caggctgatg
      841 gccacacagg ccgccacacg gatcaggaac gcgaggctca ccagggagcc ggcggcacgc
      901 cgggccaggt cgtcgtcgac accgagcacc cggacgagga ccacgccggc cagcagcggg
      961 gtggcgacga cgaaggcgag ggacgaggac gcgcgcgcca ggaggcccac gacgacgagc
     1021 tcggccatca gcaggacggc tggccagtgc ccggacggct cccgccagag ttggacggcc
     1081 agcgtaccga ccgccgctac ccagagcgtg gcccggaggt tcgcgggcgt ccacccggcc
     1141 cccacgaggc ccagagcccg gccgcccagc ccgcccccgg cggccagggc gacgacgacc
     1201 aggaggtgga ccaggaaggc caggttccag accggcgtgg tgtccgcgga ccagtacgcg
     1261 tcgagcgcga ccgcgcggac tgccgccagc aggagcaccg ccagcccacc ccaggccgcc
     1321 accggcgccg gggaccgcga ggcgagccag agcaacacca cgccctggac cgcccacgcg
     1381 agggtgaccc actgcccgtc cagcgccacc gggatggcga tggtcaggaa cgtccacgac
     1441 acgccctcgt gcaggtccac ggtgacacgg tcgtcgggaa cccgggaggc gtagtccgcg
     1501 gccacgaggc ggtagagcac ggccaagccg agcgcgtagg ccccctccca cgaggggcgc
     1561 caggcctcga gcgtgacgta gaccgcccag aagtaggccg ccgcgttggc gaccaccagc
     1621 gcgaggtcga tccggtcggc ccgccgcccc tccacccgct cgcggaagag gggtacggcc
     1681 aggaagatca ggaagagggc cgagagcagc agcagccgcg tgagggggcg cggggcctcc
     1741 ggctcgtgga gcagcgtcgg caggaggagc agcgtgctgc cgagccatgc gaggcggttg
     1801 agggatggcc aggtgcggaa gcgcgccacg agcagcacga gcgcgtcgag cacgagcagg
     1861 tacgcgagca ggttgcgctc gtcgggccgc tccacctgca gcagcacggg cgtgaggagc
     1921 ccgcccagca ccgcgagcac cgccgtggtc agccggctgc tcgcgaccgc gacggcggtg
     1981 cccaggagcg tgaccgcgaa catcgcgacg aacgcgggcg tcggcccgac gaggccgtag
     2041 agcgcgtggg cggcgaagag cgagaggtac agcacgccga ggccgccacc ggccagaccc
     2101 tcgctcaggt acgggacatc gcgccggcga tgcaggagca gcccgccgcc cagcatcacg
     2161 aggcccgcga ccactccgag ccccacgcgt gcggcgggcc cgaggaaggc attgtcgaaa
     2221 gcccacttca ggaagagcgc gaccccgacc aggatggcga cgatcccgac ccaggtggcc
     2281 cagcgcgcgc cgatgcgctg ctcgaggtcg agcccacggt gcggcggcat gggcggggcc
     2341 gcgctcggcg cctcgaggcg cggcgcgccc tccaccggcg gcccgggtgg aggcggcgcg
     2401 ggcgcggtct cgacgaccgg tgcgacgtcc ggcgcggccg ggctctcgag cggcgtgccg
     2461 gcggccggcc cgccctcgag tctccacacg cgatagacga gcccgcgcac gcgctcctcg
     2521 agcgccgcca cgcgctcgtc cgtgccggcg ggccgccgcg cgcgcgccgc gctcagggcg
     2581 acgagggcca tgacgatcgc gaccacggac aggatcagac cgacggcgat tcccgcggca
     2641 aacatgatct ggcgcgagcc tacaccaccg cgttcccggc ccggtcggat ttgtgccgtc
     2701 ccgcgcctcg acggataggc gatactaggg cccgtgctgc gaccatctcg ggcggggcgc
     2761 tgggcggtgg ccgcgctcgt cctggccgcc ctcgtcctgc ctcacgccgt cgaggcggag
     2821 ccgctccgct tccgcatcca gcccgaggcc agcgagatca gcttccgcgc aacctcgcgg
     2881 ctcatgaatg ccgacggtcg cttctcccgc ttcagcggcg acgtggtggt ggatcccgcc
     2941 gccctcacca ccgcccgcat caccctcacc atcgacgcgg cctcgctcga caccggcatc
     3001 gagatgcgcg acaaccacct gcgcagctcc gacttcttcg acgtggcgcg cttcccgacg
     3061 gtctctttcc agagcgtgcg gatcgaggcg gcgggccgac gggcgatcgt ggtgggccgc
     3121 ctgaccctgc acggcgtgac ccgggagatc gcggtgccga tcgacgtgca gatcaccaat
     3181 atcgcgctcg tggcgagcgg agagttcatc gtcaaccgcg gcgagtacgc gatgaactac
     3241 aactcgttcg tgaacccgat cggcaacgag gtccgcgtgg cgttcacctt ccgcgcccgc
     3301 attccctgag gagagacgcc atggacgcac gaaccgcggg acgtcgcatg accgagctct
     3361 tggacctgac cagccggccg gtggcggtga cattcaccgc caccgcgccc gccggcgtgc
     3421 cgcgggtcgc caaggccggg cccgccggct gcgcctactg gaaacaggcc gcggagggcg
     3481 cggtgttcta caccgaggcc gccgaccact acaactgccc ggtcggctcg ttcacccacg
     3541 gcatcgcgct gcccgccgag cgggcccggg agctcgagga cgtggtcggt acgatggtgg
     3601 gtctccagta catccggatg gaggaggtgg gggcgctacc gcgccgcccc gcgcccttcg
     3661 ggctggcggt ctacgctccg ctggccgaga cccccgcgcc gcccgacgtg gtgctggtgc
     3721 gcggccgcgc gcgcgagatc atgctggtgg ccgaggccgc gcgcgccgcc gggatcgcgg
     3781 gcgacggggc cgcgatgggc cggcccgcct gcgcgatgat ccccgcggtc ctgggcgggc
     3841 ctgccggcgt caccagcctc ggctgcatcg gcaaccgcgt ctacaccggt ctgggcgacg
     3901 acgagctcta cttcacgatc ccgggctcga agatcggcga ggtggtcgag cggctcgaga
     3961 cggtggtgga cgccaaccgg gcgctccaga cgtaccacga ggggcgccga gcgaccgtct
     4021 agccccctca ccctgccctc tccccacagg ggagagggag atcaccgatc gggctataat
     4081 cagtccccca tgaacgcgcc cctcgccggc ctgctcgtcg ccgatctcac ccagaacgtg
     4141 gcgggcccgt actgcaccca gatcctgggc gacctcggcg ccgagatcgt gaaggtagag
     4201 cgcctcggcc gcggggacga cgcgcgcgcg tgggggccgc ccttctgggg ccaggagagc
     4261 gccaccttca tgagcgtcaa ccgcaacaag cggagcctcg cggtggatct caaggccgac
     4321 gaggggcgcg cggtggtcga gcggctcctc ggccgcgccg acgtcttcgt gcagtcgctg
     4381 cgggccggcg ccgcggagga gctggggctg ggctacgcac gggtcgccgc gctcaacccg
     4441 cgcctggtct actgctcggt caccgccttc ggcaccgagg gcccgctgaa ggaccggccc
     4501 ggctacgacc cgctgatgca ggcctacggc ggcatcatgt ccatcaacgg ccatccgaac
     4561 cagccgccgg cgcgcgtgcc ggtctcggtg gtggacatgg gcaccggcat gtgggcggtg
     4621 atgggcatcc tggccgcgct gcgcgagcgc gaccgcaccg gccacggggc tgaggtcacc
     4681 accgcgctct tcgacaccgc gctcgcgtgg tcggtcttcc agatgagcca ctacttcgcc
     4741 accggcgagg tcccgcggcc gcagggctcg ggcaccgcca tgatcgtgcc ctacgaggcc
     4801 ttcccggtcg tggacggctg ggtcatgatc gcggccggct cggacgccct cttcgtgaag
     4861 gcgaccaccg cgctcgaggt gccggagctg gcccgcgatc cgcgcttcgt ggacaacccg
     4921 cggcgcgtcg agcaccgggg agcgctgcag gaggccctgg cccgggtcac gcgggtgatg
     4981 accgcggagc agctgctggg gcgcctccag cgggcgggcg tgccgtcggc gcgcgtgtcc
     5041 acgatggacc aggtcgcggc cgagccgcag acggaggcga gcggcatgct cccggcggtg
     5101 aagcacccgc gcctgcccga ctaccgcgcg gtggccctgc cgatcaagtg ggacggcgag
     5161 cgcccggccg cctcgcgcgt gccaccgctg ctcggggagc actcggccga ggtgctggcc
     5221 gagctgggct acgacgacgc ggccatccgg gggctcgtgg agcgccatgt catccagcta
     5281 taggacgctg aacgtcgcca cctccgccga cggccacgtg gtcacggtgg agctgaaccg
     5341 gcccgaggcg ctcaacgcga tgaacaccgc gatgggcgag gacctgctcc gctgcttcga
     5401 ggatctccat cgcgaccgca cggtgcgcgc ggtggtcttc accggcgcgg gcaccaaggc
     5461 cttctgcgtg gggggcgatc tgaaggagcg ggagggcatg accgacgaga cctggcgcgc
     5521 ccagcacgtg atcttcgagg ccgcggccgc gcgcgtgctc cactgcccgg tgccggtgat
     5581 cgcggcggtc gagggcttcg ccatgggtgg cggctgcgag ctggccgtgc tctccgattt
     5641 cgtggtctgc ggcgagaccg cggtgttcgc ggtccccgag gtcacgcgcg gcatcttccc
     5701 ggggatcggc gggacccagc tcctgccgcg gatcctgggc gcgccgatgg ccaaggagat
     5761 gatcttcacc ggccggcgcg tggacgcgcg cgaggccaaa gccctcgggc tcgtcaatca
     5821 cgtggtgccg gccggccagg cgcgggccaa ggcgctggag atcgccgcga ccatcgcgga
     5881 caatgggccc atcgcggtcc gccaggccaa gaaggcggtc aaccggggcg gcgagaccga
     5941 cctggagacg ggcatgatcc tggccatcga ggcctacaac aacacggtca ccaccgacga
     6001 ccgcctcgaa ggcgtccgcg cgttcaacga gaagcgtaag ccccggttca cggggaaata
     6061 gggcaagaac gaagagggga taagcatggc agacatgacg ttcgtgctca gcgacgagca
     6121 gaaggccctc aaggccgcgg tctacgacct ctgcaagcag tacccggccg agtactggcg
     6181 cgacctcgac agccggcgcg agtacccgga ggccttcgtc agcgagctga ccaaggccgg
     6241 ctacctcgcg gtgctcatcc ccgaggagta cggcggcggc gggctcggca tcatggaggc
     6301 cgccctgatc ctcgaggaga tcgggcgggc gggcggcaac ccggcggcct gccacgccca
     6361 gatgtacatc atgggcaccg tgctgcggca cggcaccgac gcccagaagc gcgagtacct
     6421 gccgaagatc gccgcgggcg agctgcggct gcaggccttc ggcgtgaccg agcccaacgc
     6481 gggctccgac accaccaagc tccagaccac cgcggtccgg aagggcgacc gctacgtggt
     6541 gaacggccag aagatgttca tctcgcgcgt gctgcagtcc gacctcatgc tgctgctcgc
     6601 ccgcaccacg ccggtggacc aggtcaagaa gaagacggat ggcctcagcg tcttcctcat
     6661 cgacatccgg gagctgaagg gcaaggggct cgacgtgcgc ccgctccgga tgatgatgaa
     6721 ccactcgacc aacgcgctct tcttcgacaa catggagatc cccgccacga gcctcatcgg
     6781 ccaggagggc cgaggcttct cctacatcct cgacggcatg aacgccgagc gcatcctggt
     6841 cgcttccgac tccctcggcg acgcgcgctg gttcaccgag aaagcggtgg cctacgcggg
     6901 ccagcgcgtg atcttcggca agcccatcgg ggccaaccag ggcgtgcagt tcccgatcgc
     6961 caaggcgcac gtgagcatcg aggccgccga tctcatgcgc accaaggcgg cgcggatgtt
     7021 cgacgcgggc atcccgtgcg gccccgaggc caacatggcc aagtacctgg cctcggaggc
     7081 ggccatcgag gcgggcaacg cctgcatcga ctgccacggc ggctacggct tcgcggagga
     7141 gtacgacatc gagcgtaagt tccgcgagtc gcgcctctac cgggccgcgc cgatcaacaa
     7201 caacctggtc atcgcctacg tcggggagca cgtcctcggc atgccccgct cttattgagg
     7261 gtagtcccca cctcattccc ctctccgctc aggggagagg gtagggtgag gggtggtctg
     7321 acgagaggag ctatgctagg tcccatcctc gaaggcgagc gcgtccggct cgagccgccc
     7381 cggccggagt acttcccggc ctaccagcgg tggttcgccg acatggaggt gacgcgttac
     7441 ctgctctacc gcttcccctt caccacgagg tgggaggagg agtggctgga gcaggcgggc
     7501 cgggacccgg cccaggtgat ctgggcgatc gtgctcaagc acaacggccg cttgatcggc
     7561 aacgccgcgc tcgagcatat cgattggcgc gaccgccgcg ccgagagcgg catcgtgatc
     7621 ggcgagaagg acgagtgggg caagggctac gcgggcgagg ccatgcgcct gcgcacccgc
     7681 tacgccttcc gcgagctggg gctcgagacc gtcaccacgc gcgtggtcct caccaacgcg
     7741 gcgagccggc gggggctcga gcgggccggc tatcgccagg ccggcgtgct gcggcaccac
     7801 tacttcgtgg acggccgctg gcacgacgcc tgggtgggtg agatcctcaa ggacgagtgg
     7861 gaagcgacac aggagacgac acaatgaagc tgcccagaca cgtgacgatc tgcgaggtcg
     7921 ggacgcggga tggattccag atcgagcccg acttcatccc gaccgaccag aagatcgaca
     7981 ccgtgaaccg cctgtccggg gccggcatac cccgcatcga ggtgacctcg ttcgtgtcgc
     8041 cgaaggcgat cccccagctc cgggacgccg cggaggtcat ggccgccatc acccggcggc
     8101 ccggcaccac ctactcggcg ctggtgccca acgacaaggg ggcggtgcgg gcggtggacg
     8161 cgcgggtgga tgagatccac accgtggtct cggcgagcga gagccacaac ctcgccaacg
     8221 tgaagatgac gatcgcggag tcgctcacca agctgggcgc ggtgtcggag gtggcccggc
     8281 gcgcgagcgt gccggtcgtc tccggcatct cctgctcctt cggctgcccg ttcgagggcg
     8341 acgtgccggt ggcccatctc gagtcggtgg tcgcgcgcct agtggacatg ggcgcccgcg
     8401 gcatcggcct ggccgacacc accggcatgg ccaaccccgt gcaggtcggc cgcgtgctcg
     8461 agcggctggt gccgcgcttc cccggcgtcg agtggacact gcatacccac gacacgcggg
     8521 cgatggcgat cccgaacatc ctcaccgcga tggaatacgg cgtgacccac ttcgacgcct
     8581 ccatcggcgg gctcggcggc tgccccttcg ccccgggcgc gtccggcaac gtgtgcaccg
     8641 aggacctgat ccactgcctg cacgcgatgg gcgtggagac cggggtggac ctcgacgcgc
     8701 tgatcgagac ctcgaagcgc gtgcaggcga tcgtcgggcg cgagctcccc ggccagctcg
     8761 tgaaggcggg caagtgggac cgccgctacc cgctccccga cggggtgcgc gagcgcatcc
     8821 ccgcgcgctc atgaccaaga ccgcgacgcg aatgctccga ttgatggacc gggcagtcac
     8881 ccactggcgc atcccggtca tcggccgcga gaaggggcgc gtgctgcggc gcctgctgct
     8941 gaagcaccag ccccgacggg ggatcgaggt cggctcgctc ttcggttact ccgccatcct
     9001 ggtcgcgggc cacctgcccc gcggcggccg cctgacctgc gtggagcaga atccctacct
     9061 cgccaagttc gtggccagca acgtggagga ggcggggctc gccaagcgcg tgaaggtggt
     9121 ggtgggcgac gcgctccggg tgctgccgct gctgcgcggt ccggtggact tcctcctgct
     9181 cgacgccaag aaggaggact acctcgacta cctgcgcgcg gtggagccgc gcctgcgcaa
     9241 gggcgccctc gtcgtcgccg acaacaccgg gatctaccgg cgcgacgtga agccctatct
     9301 cgaccacgtg cgcgcgagcg gacggtacga gagccgcgag cacgacttcg gctccgactg
     9361 catggaagtc tccgtgttcc gcgggtagcg ccccatgctc ggcgagcagc acgacccgga
     9421 attcctcaag cacctcttcg agaccaccga gatcctgcgc aagccggtcg ccggcatcat
     9481 cgcgggctac cacgtgctgc cctacatcct ggtgggcccg caggagacga agccggcctc
     9541 gtcggtggag atccgcggca agatcaaggt ctcgccgcgc ctggtgctgg cccccggacg
     9601 cgccggcccg acctacggcg atctcttcaa ggagaacgag ctgatggacg aggccctcat
     9661 cgcgcgggtc ttctccttcg cctactcgaa tcgccaccag gtgaccctcg agagcgagga
     9721 cctcaagatc cagcgctggg accgcaaccc gcaggcccag gtcgagcggg ccctcgacga
     9781 gctcagccag cgagaagtga tcaataccgg cgtgatcctc gcccccaacg tgcggttcta
     9841 cccggtctcg ctcgaccgct tcatcaccga gatcctcgac caggagttca agtcatgacc
     9901 accgagacct acgactggga cgccatcgtc aacggcctca accagtacct gcgcctccgc
     9961 acgatcccca tcggcatgaa gctcttcgag accgtcgagg agatggaggc catccccaag
    10021 atccgccggc cgaagagcaa gcacaccacc gaccagatcg tggcccaggc gcgccagctc
    10081 ggctggacgg tcggcatcac gatggcggac ctgatcggcg cccagtgcgg cgcggtgatc
    10141 gggctccatc cccaggacgc ggagtggctc tccggcaagc ggatggccgg cgtgtggttc
    10201 aagacccagg aggacgcgag cctccatcag cacgcgatgg actgcgtgcc ccacgggcgc
    10261 taccgcgcga tggcggtggc cccgctcacc tccgggcgcc tcgatccgcc cgacatctgc
    10321 ctcatctacg gcacgcccgg ccagatgatc ttcgtcatca atgggctgca gtggatcggc
    10381 tacaagaaga tgtcgttcac gtcggtgggc gagtcggcct gcgcggattc ctggggcaag
    10441 gcgctcaaga ccggcgagcc cgccctgacc attccctgct acgcggagcg ccgctacggc
    10501 ggggtggcgg acgatgagct cctgatggcg atccccccgc gcttcctccc caaggtcgtg
    10561 gagggcctgg ccgcgctcag caagaacggt ctccgttacc cgatcccgcc ctacggcatc
    10621 cagagcgacg cgggcgcggg cctggcggtc agctacccgg gcgccccggc ggggtcggcc
    10681 gggcgctgac gggtcagagc ccgaccgcct cggtcaccag ccgggccagc tcgcgccgca
    10741 gggcgtcccg ctccgacgcg cgcgccggct cgtcgtggag gttgcgcagc tcgtaggggt
    10801 cggcggcgag gtcgtagagc tcgtccaccc cgtcgcggtt gacccagtgg atcagcttgg
    10861 cccgctcggt gcgcaccgcc ttgtaggtca tcccgaccag ccagggcatc gcgttctcgc
    10921 tccagtactc gacgaggaac gaggtgcgcc agtcgggtgg gcgccggcca cccaggagcg
    10981 ggaccagcga gcgtccctgc acctgcgggc ccggcgtgcc gcccgcgagc gcgagcatgg
    11041 tg
//
LOCUS       DASUFA010000218         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1178035, whole genome shotgun sequence.
ACCESSION   DASUFA010000218 DASUFA010000000
VERSION     DASUFA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178035"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFX87_08065"
     CDS             complement(<1..408)
                     /locus_tag="VFX87_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4994908.1"
                     /translation="MAARRLGRVVTLAILAGWAGGAFAQVPPSAEDRAGYTGLFAAAA
                     RGDAAQTKGLLAGGQKPDGRDAHGRTPLHVAAFGGHHEAMQVLAAAGADPNALERDRY
                     DIVTIAAVANDVPTLRVALALGASARNVTSRYDG"
     gene            complement(436..708)
                     /locus_tag="VFX87_08070"
     CDS             complement(436..708)
                     /locus_tag="VFX87_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009631806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX4994909.1"
                     /translation="MLYMVIEYFNAGAAAEIYRRARERGRQLPPGLEYVDSWVDLDYF
                     RCFQLMRTEDRALFDVWIQAWSDLGHFEVIPVRTSAEAAQHIAGQR"
     gene            complement(713..1675)
                     /locus_tag="VFX87_08075"
     CDS             complement(713..1675)
                     /locus_tag="VFX87_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994910.1"
                     /translation="MARTALFLNPGADLAPALALARRADALGYESLWVTHGIGRDALL
                     TLSAFAHVAPHAGLGSGVIPIYPRHPVLLAQEALTLSDTSGGRLRLGIGVSHRPVMEG
                     AMGLDMGRPLDVIREYVTVLRGALTGKVRHEGPRYRVNWQSGLPRIPAAPPILLAGLA
                     PRMLELAGEIADGVVLWLAAPAYVRDHALPAIRRGRARAGKPLDGFEIVAAAPAALTA
                     DRTAAITLFRTELQRYLVLPFYRAMLEQSGFTADVQAFDRAPGPDAVSDRLVAALGGI
                     GDFRSVSAFVSAYRDAGVTLPAVRPIGFPDAPHYLPTVEAAVAA"
     gene            complement(1681..2313)
                     /locus_tag="VFX87_08080"
     CDS             complement(1681..2313)
                     /locus_tag="VFX87_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX4994911.1"
                     /translation="MAPATSLRAVIFDFGGVLWDMRWDVARDLDRTHGLPKSSLFETL
                     YRSPAWSGIECGVGDPAEWAEGAHRELERRAGRALPRLHEEWRRAQVAIGVNIALVRA
                     LRPPYRCSVLSNADLSLRGRLKEELALDHLFDDIVVSAEVGMAKPHPEIFRLAADRLG
                     LSPEACVFVDDWDKNVDAARAVGMQAVLHRVDQGHDLRAQLAVLGVAPGA"
     gene            complement(2297..2560)
                     /locus_tag="VFX87_08085"
     CDS             complement(2297..2560)
                     /locus_tag="VFX87_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994912.1"
                     /translation="MTGRYCLLLMVAASLLPVGCSSERDRQWYKPGANYTVSEFTRDR
                     DECTKGRELDEQCMKQRGWVPLTSDREPSTSVKPAQNPRGPRY"
     gene            2589..>3133
                     /locus_tag="VFX87_08090"
     CDS             2589..>3133
                     /locus_tag="VFX87_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX4994913.1"
                     /translation="MLDTTIAGSLPKPEWLAPPNTLWAPWRLEGRALEEGKRDAVLLA
                     LREQEQAGIDIVTDGEQTRRHFVWGFVECLDGIDFSKMVTIGIRADRYKANVPTVTGP
                     VRRRGSIHGPIHGEDVRWARANTGRRLKLTIPGPMTIVDTIHDAHYGSRERLAMDLAR
                     LINEEARELEALGLDMLQLDEP"
BASE COUNT          455 a         1140 c         1146 g          392 t
ORIGIN      
        1 gccgtcgtag cggctggtca cgttccgggc gctcgcgccc agggcgagcg ccacccgcag
       61 ggttggcacg tcgttggcga ccgccgcgat cgtgacgatg tcgtaccggt cgcgctcgag
      121 cgcgttcggg tctgcgcccg cggcggccag cacctgcatc gcctcgtggt gcccaccgaa
      181 cgcggccacg tgcagcggcg tgcggccgtg cgcgtcccgc ccgtcgggct tctggccgcc
      241 cgcgagcagc cccttggtct gggctgcgtc gccccgggcc gcggcggcga agagccccgt
      301 gtagccggcc cggtcctctg ccgacggcgg cacctgcgcg aaggctccgc cggcccagcc
      361 ggccaggatc gcgagcgtca ccacgcggcc gagcctgcgc gccgccatca cgacgcctcg
      421 acgtcggcca cgcccttagc gctggccggc gatgtgctgg gccgcttcgg cggaggtccg
      481 caccgggatg acctcgaagt gccccaggtc gctccacgcc tggatccaga catcgaacag
      541 cgcccggtcc tcagtgcgca tgagctggaa gcagcggaag tagtccaggt ccacccagct
      601 atccacgtac tcgagcccgg gtggaagctg gcggccgcgc tcgcgggccc ggcgatagat
      661 ctcggccgcc gcgcccgcgt tgaagtactc gatgaccatg tagagcatcc gatcaggccg
      721 ccaccgccgc ctccaccgtc ggcaggtagt gcggggcgtc ggggaagccg atcgggcgca
      781 ccgcgggcag cgtgacgccg gcgtcgcgat aggcgctgac gaaggcggac accgaccgga
      841 agtcgccgat gccgcccagc gcggccacga ggcggtccga gaccgcgtcc gggcccggag
      901 cccggtcgaa ggcctgcacg tcggcggtga acccgctctg ctccagcatc gcgcggtaga
      961 acggcagcac caggtagcgc tgcaactcgg tgcggaagag cgtgatggcc gccgtccggt
     1021 ccgcggtgag cgccgcgggc gccgccgcca cgatctcgaa gccgtcgagg ggcttgcccg
     1081 cgcgggcgcg cccccggcgg atggcgggca gcgcgtggtc gcgcacgtag gccggcgcgg
     1141 ccagccagag cacgacgccg tcggcgatct cgccggccag ctccagcatg cgcggggcca
     1201 gcccggccag caggatcggc ggagcggccg ggatcctcgg gagcccgctc tgccagttga
     1261 cgcgatagcg cggaccctcg tggcgcacct tgccggtcag cgcgccacgc agcaccgtca
     1321 cgtactcgcg gatgacgtcg agcggccggc ccatgtcgag gcccatcgcg ccctccatca
     1381 ccgggcgatg gctcaccccg atcccgagac gcagacggcc cccgctcgtg tcggacagcg
     1441 tgagcgcctc ctgggccagc agcaccggat gacgggggta gatcgggatg acgccggagc
     1501 cgagacccgc gtgcggggcc acgtgcgcga aggccgagag ggtcagcagc gcgtcgcgcc
     1561 cgatgccgtg agtgacccag aggctctcgt agcccagggc gtcggcgcgc cgcgccagcg
     1621 ccagcgcggg cgccaggtcg gcgccggggt tcaggaagag cgcggtgcgc gccatcgcgc
     1681 tcaggcgccc ggggccacgc ccagcaccgc gagctgggcg cgcaggtcgt ggccctggtc
     1741 cacgcggtgg agcaccgcct gcatgccgac ggcgcgcgcc gcgtccacgt tcttgtccca
     1801 gtcgtccacg aacacgcacg cctcgggcga caggcccagg cgatcagcgg cgaggcggaa
     1861 gatctccggg tggggcttcg ccatgccgac ctcggcggag accacgatgt cgtcgaagag
     1921 gtgatccagc gccagctctt ccttgagccg gccgcgcagc gagagatccg cgttgctcag
     1981 gaccgagcag cggtatgggg gccgcagcgc ccgcaccagg gcgatattga cgccgatggc
     2041 cacctgcgcc cgccgccact cctcgtgcag gcgcggcagc gcgcggccgg cgcggcgctc
     2101 gagctcccgg tgggcaccct ccgcccactc ggcggggtcg cccacgccgc actcgatgcc
     2161 gctccaggcc gggctgcggt agagcgtctc gaacagggag gacttcggca gaccgtgggt
     2221 gcggtccaga tcgcgcgcca cgtcccagcg catgtcccac aggacaccgc cgaagtcgaa
     2281 gatgacggcg cggaggctag tagcgggggc cacgcggatt ctgggcgggc ttgaccgagg
     2341 tggacggctc gcgatcgctg gtgaggggca cccagccgcg ctgcttcatg cactgctcgt
     2401 ccagctcgcg gcccttggtg cactcgtcgc gatcgcgcgt gaactcgctc accgtgtagt
     2461 tggcgccggg cttgtaccac tggcggtccc gctcggagct gcagccgacc ggcaggagcg
     2521 acgcggcgac catgagcagg aggcaatacc gtccggtcat gctcggcatg gtaccatgcc
     2581 gcccaccgat gctggacacc accatcgccg gcagtcttcc gaaacccgag tggctggccc
     2641 cgcccaacac gctgtgggcg ccttggcggc tcgagggccg cgcgctcgag gagggcaagc
     2701 gcgacgcggt gctgctggcc ctgcgcgagc aggagcaggc gggcatcgac atcgtcaccg
     2761 acggcgagca gacccgtcgc cacttcgtgt ggggcttcgt ggagtgcctc gacggcatcg
     2821 acttctcgaa gatggtcacg atcggcatcc gggccgatcg ctacaaggcc aacgtgccga
     2881 cggtcaccgg gccggtgcgg cgccgcggct cgatccacgg cccaatccat ggtgaagacg
     2941 tgcggtgggc ccgggccaat accgggcgcc ggctcaagct gacgatcccg ggaccgatga
     3001 cgatcgtgga caccatccac gacgcgcact acggcagccg cgagcggctc gcgatggatc
     3061 tggcccggct catcaacgag gaagcgcgcg agctggaggc gctcgggctc gacatgctgc
     3121 agctcgacga gcc
//
LOCUS       DASUFA010000219        11428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_55522, whole genome shotgun sequence.
ACCESSION   DASUFA010000219 DASUFA010000000
VERSION     DASUFA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55522"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFX87_08095"
     CDS             <1..664
                     /locus_tag="VFX87_08095"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994914.1"
                     /translation="TEELGVRAYLALWTTNLGEGLAAAGQLDRARTVAQRALDLALRH
                     KERGHQAGALRLMGELAALSEPPAVGEAEGYYAQALSLAEELEMRPLQARTHLGLGRV
                     LRLAGDRERAEDHLATALGLLREMDMRFWLSRAAEELLHLGHLFVVARHNIQLYDYLK
                     REYAGEPVTVILDRRHGERRERAEATASERRAADRRRQVRVDESIRTRGFAVVPEIIG
                     GA"
     gene            complement(648..902)
                     /locus_tag="VFX87_08100"
     CDS             complement(648..902)
                     /locus_tag="VFX87_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994915.1"
                     /translation="MIKTGRIEFYDRPTAWGLIIGDDQDLYVIRGRDGRGTAPRVGER
                     VSFEPQSGTDGKRATEVRLVSRGLTSIGREAPPVGVRPLR"
     gene            996..1820
                     /locus_tag="VFX87_08105"
     CDS             996..1820
                     /locus_tag="VFX87_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX4994916.1"
                     /translation="MTVSQSDKAVRFRALHEAPGAFVIPNPWDAGSARILAALGFQAL
                     ATSSGASAGILGRRDGKVTREEALAQARAIVEATDLPVSADLEKCFADAPAAAAETIR
                     LAASVGLVGGSIEDATGNKDKPLFDIGAATERVVAAVAVARALPFPFTLTARTESFLR
                     GNPNLDDVIRRLQAFEKAGADVLMAPGLPDLAAVRAVCAAVKKPVNFMVGIKGKSFTV
                     AELTAAGVKRISLATSLYRTAMSGLIDAAREVKAAGTFGYVDRGVSSADLAGFMEG"
     gene            complement(1842..2882)
                     /locus_tag="VFX87_08110"
     CDS             complement(1842..2882)
                     /locus_tag="VFX87_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX4994917.1"
                     /translation="MATDSPTVRSLKIRLLLPLTLVLAVVVIGTLGYRWLWRDVDGTW
                     MDALFMTVTTITTIGYGEIRPLTTAGRLFTIFLAFTGIGSLFYTLGVVMEYLVGVRLA
                     DPMGRRRMERRIGELTGHVIVAGMGRVGRQAALELHEAETRFVVVDPSEQAMAHATER
                     GYLALQGDATEDAVLKRAGIARARGLIVTTGNDATNVYVVLSARVLAPELYIVSRAAD
                     EAGVTKLGRAGANRAVSPYAIGGHRLAHLMLSPAVVDFFETALRRGDDALNIEDLAMA
                     ADSPSVGKTLDGLDIRRATGATVLAVLRDGNPLASPPGDLTLAAGDHLLALGTGDQLR
                     RLEKLITAGAGS"
     gene            2984..4456
                     /locus_tag="VFX87_08115"
     CDS             2984..4456
                     /locus_tag="VFX87_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994918.1"
                     /translation="MKILLTGATGYVGGRLLKALEARGHELRCLARNPANLASRVGPG
                     TRVVPGDVLDAASLAAALAGIETAYYLVHSLGDASGFEEREKAGARNFATAARAAGIA
                     RIVYLGGLGDARADLSPHLRSRHEVGRILRESGVRTIELRASIVIGSGSLSFEMIRAL
                     SEHLPVMIMPRWVSVLAQPIAIQDLIEYLVQALDVPIEDSTIVEIGGADRLSYADLMR
                     EYMRQRHLRRLMIPVPVLTPRLSSLWLGLVTPLYARVGRKLLESMKNPTVVQSDAAKR
                     LFRVEARGAAAAIRAALENEDREFAETHWFDSLSASGTPNGSGGSHPGPRLIDRRERQ
                     ADASPEACFAVVARLGGRGGWLAFDWLWRLRGLLDLLVGGVGMRRGRPEHRALVVGDT
                     LDFWRVEAYEPPRRLRLFAEMRLPGRAWLEFVVEPAGAGSRITQTALFDPVGVLGRVY
                     WYGVFPLHGLVFGAMIEAIAKRAAVVEGRPESRTGPRQSP"
     gene            complement(4472..4547)
                     /locus_tag="VFX87_08120"
     tRNA            complement(4472..4547)
                     /locus_tag="VFX87_08120"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4512..4514),aa:Ala,seq:ggc)
     gene            complement(4612..5142)
                     /gene="tpx"
                     /locus_tag="VFX87_08125"
     CDS             complement(4612..5142)
                     /gene="tpx"
                     /locus_tag="VFX87_08125"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX4994919.1"
                     /translation="MADRAGAVMFKGNPITLGGPEIKAGSDAPDFTAVDNGLQPVKLS
                     QAKGKVIVLSAVPSLDTPVCDTETRRFNQEAGKIGAGVEVWTVSMDLPFAQKRWCGAA
                     GVTAVKTLSDFRERSFAQNYGVLVKEGPLAGLTARAVFVVGKDGKVKHAEYVKEITTE
                     PNYDAALNAAKQAAGA"
     gene            5235..5321
                     /locus_tag="VFX87_08130"
     tRNA            5235..5321
                     /locus_tag="VFX87_08130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5269..5271,aa:Leu,seq:caa)
     gene            5346..6350
                     /locus_tag="VFX87_08135"
     CDS             5346..6350
                     /locus_tag="VFX87_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX4994920.1"
                     /translation="MTAATRSALHTPLCGRLGIRYPICQAGMAFVARAQLAAAVSAAG
                     GLGVIAAAHGTAADLREEIHRVRELTDRPFGVDILFASVRTSGQETERFTDAVKGWID
                     VTLEERVPVLIAGLGNPGPVTAEAHRLGITVMALCGNVKQARDHAANDVDVIIAQGHE
                     GGGHTGRVGGVVLTPAVVDAVAPRLVVAAGGLADGRGLVAALALGAAGVWMGTRFIAT
                     REAYGHDNYKQKIATIDEEGTVVTRAVSGKPNRQIRNAFTREWESRQAEIQPFPVQYE
                     RVGKPASIRAREEGDVEHGSAAAGQSAGLIYDVRPVADVMASIVNEAEAILHRLGARA
                     "
     gene            6347..7150
                     /locus_tag="VFX87_08140"
     CDS             6347..7150
                     /locus_tag="VFX87_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994921.1"
                     /translation="MTRPLDGRVAVVTGGSRGIGRSIALRLARDGAHCAITYRKDASA
                     AARTVGELEALGVRAVAERLELAEPEQVALVFERIGAVLGRVDVLVANAAATAFRPVL
                     EQKPHNVRRTFAISVDAFVAMAQAAVPLMKGRPGRIVLISGIDSFQAMGGHSVLGAAK
                     AAAEGLVRGLALELGPLGVTVNAVTPGFVKTDSSTYYVTQGLGLDYERATARLLAATP
                     VRRLGTPEDVAALVAYLVSDAASFLTGQCVVIDGGLTITSPLSRMEDGP"
     gene            7169..9181
                     /gene="shc"
                     /locus_tag="VFX87_08145"
     CDS             7169..9181
                     /gene="shc"
                     /locus_tag="VFX87_08145"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX4994922.1"
                     /translation="MPSSWFEPLPARATYQAEAHGEHRAALAEAAAAACARTAPTLLA
                     MQKPEGYWVGDLLADSTLESDYVLLQLWLHPPQDGEWKPPSWDRIQRARKAILAQQRS
                     DGGFNIYPDGPADVNATIKAYIALKLAGLGAHSEPLQRARAAILHLGGIQEANSYVRI
                     NLSLFGLYPKRHVPTIPVELALLPGGLIYEMSSWTRAIVMPLTIIQAKTDTRPVPAGF
                     TLDELVVPGKSFKLPKRDRLSALFRQLDIALKMWATRGPEMIRRPAIREAEKWVLDHT
                     RNSDGLGAIYPSMMYLIMALDALGYPRDHPDLIEAIRQYEGLLTETPEAFYFQPCVSP
                     VWDTAIAMFALGEMGAVAPAAMRRGADWFIAKEIRRRGDWSVKRPDLEPSGWAFEFAN
                     EHYPDIDDTAMVLLAMQHAQASDAAAQERCERRAVAWLVGMQSGDGGWAAFDADNNWA
                     FLNRVPFADHNAMLDPSCPDITGRVLEALCRRGFKRSDPSIEKGVGFLLETQERDGSW
                     YGRWGVNYLYGTFLALRGLRAAQDPGASKAVWKAGKWIASVQNEDGGWGESCASYNEG
                     RFVPAPSTPSQTAWALLGLIAAGQGDTEVLVRGVRHLVDTQKPDGTWDEHLATGTGFP
                     NVFYLRYTLYRTYFPLLALTQARRAIDEAERTLRLVQDAAPLHPLR"
     gene            complement(9208..10575)
                     /locus_tag="VFX87_08150"
     CDS             complement(9208..10575)
                     /locus_tag="VFX87_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX4994923.1"
                     /translation="MRYHFSGIAGAGMSPLARLMRARGHAVQGSDRSFDQGKNAEVAA
                     GLSALGIVVKPQDGSAVTADLDRFIYSTAVEAETPEVRAARALGLERVPRPALLAEVV
                     DAGRPGVAIAGTSGKSTITGMVGWLTREAGVAATVLGGAALAGDRTSGCFLPGPVDGP
                     VAAEACESDGTLTGYHAALGLIHNISRDHGEVDALRPQFEAFAKRSGRLLVNSASPEA
                     ALLGRAVGAHGYGLGPDADTPLEVTSTGPHRARGTLHLPTGPLVLDIPQPGLHNLENA
                     AAAALIALDLGIAPATVAALLARFPGVARRFEVIGVTAAGIRVVDDYAHNGEKLRAAI
                     TAAQAGASRVVAVFQPHGFGPARFLRPELRELMPRLLRPADRLCYAEIFYAGGTVARD
                     ISSRMLADDLPAALRCGYASSHRAVIDWLRAESRPGDTVLIMGARDPELPGLARAAFS
                     ALQTA"
     gene            10734..>11428
                     /locus_tag="VFX87_08155"
     CDS             10734..>11428
                     /locus_tag="VFX87_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994924.1"
                     /translation="MSIAEAEAVVVKGRFCGSPRTASGGYIAGLLGRHVDGAARVSLD
                     SPIPVDHAFGAERLADGRVVLLDGAAVLAHAEPATLQIEPPTPPSFAEAQWASERYLG
                     FTEHASPECFVCGPAREWGDGLAIFPGPLPGRHLVAAPWAPDPTLFDKHGTVRPEFVW
                     AALDCPSGFALLEAFGRRKAVLSQLTVKLIRPLEVGARWIVMGWPFAADGRNLLGASA
                     IVSESGELHALASA"
BASE COUNT         1566 a         4050 c         4143 g         1669 t
ORIGIN      
        1 gaccgaggaa ctgggggtgc gcgcgtacct cgcgctctgg accacgaacc tgggcgaggg
       61 cctcgccgcg gccgggcagc tcgaccgcgc ccgcaccgtg gcgcagcgcg cgctcgacct
      121 ggccctgcgg cacaaggagc gcggccacca ggccggcgcc ctgcgcctga tgggcgagct
      181 cgccgcgctg agcgagccgc ccgcggtggg ggaggcggag ggctattacg cacaggcgct
      241 gagcctcgcc gaggagctcg agatgcgtcc gctccaggcg cgcacccact tggggctggg
      301 gcgggtcctg cgcctcgcgg gcgaccgcga gcgcgcggag gatcacctgg ccaccgccct
      361 gggcctcctg cgcgagatgg acatgcgctt ctggctgtcg cgggccgccg aggagctcct
      421 gcatctcggg cacctgttcg tggtggcgcg ccacaatatc cagctctacg actacctcaa
      481 acgcgagtac gcgggcgagc cggtcaccgt gatcctcgac cggcgtcatg gcgagcgccg
      541 ggagcgcgcg gaggccaccg ccagtgagcg gcgggccgcc gaccggcggc gccaggtccg
      601 ggtggacgag tccatccgca cgcgcgggtt cgcggtcgtc cccgagatca tcggaggggc
      661 ctgaccccga ctggcggcgc ctcgcgcccg atcgacgtga gaccccggct caccagtcgc
      721 acttccgtcg cgcgcttgcc gtcggtgccg gactgcggct cgaacgagac gcgctcgccg
      781 acccggggcg ccgtcccgcg cccgtcccgt ccccggatca cgtagagatc ctgatcgtcg
      841 ccgatgatca ggccccacgc ggtgggccgg tcgtagaact cgatgcgccc cgtcttgatc
      901 atggcgggat cctagggcgc cgcgcgcggc cggcacaatg tccagaatct cggacacgag
      961 tgcactagaa taccggcggt ccaaggaggc ccatcatgac cgtcagccag agcgacaagg
     1021 ccgttcgctt ccgcgccctc cacgaggcgc cgggcgcatt cgtgattccg aacccgtggg
     1081 acgccggctc ggcgcgcatc ctcgccgcgc tcggcttcca ggcgctcgcc acctcgagcg
     1141 gcgcctctgc cggcatcctc ggccgccgcg acggcaaggt gacgcgtgag gaggcgctcg
     1201 cgcaggcgcg cgccatcgtg gaggcgaccg acctgccggt gagcgcggat ctcgagaagt
     1261 gcttcgcgga tgcgccggcg gccgcggccg agaccatccg gctcgccgcg tcggtggggc
     1321 tggtgggcgg ctcgatcgag gacgcgaccg gtaacaagga caagccgctc ttcgacatcg
     1381 gagccgctac cgaacgggtg gtcgccgcgg tggcagtggc gcgcgcgttg ccctttccgt
     1441 tcacgctgac cgcgcgcacc gagagcttcc tgcggggcaa ccctaacctg gacgacgtga
     1501 tccgacgcct gcaggccttc gagaaggcgg gggccgacgt gctcatggcg cccggcctgc
     1561 ccgacctggc cgcggtgcgc gcggtatgcg ccgcggtgaa gaagccggtc aacttcatgg
     1621 tgggcatcaa ggggaagtcg ttcacggtgg ccgagctgac cgcggcgggc gtgaagcgga
     1681 tcagcctcgc gacgtcgctc taccgcaccg ccatgagcgg cctgatcgac gcggcccgcg
     1741 aggtgaaggc ggccggcacc ttcggctacg tggaccgcgg ggtgtcctcc gcggacctcg
     1801 ccgggttcat ggagggttga ccgaggcggc cgcctccgcg gtcacgaccc ggcgccggcg
     1861 gtgatcagct tttccagccg ccggagctga tctcccgtgc ccagcgcgag caggtggtcg
     1921 ccggcggcca gggtcaggtc gccgggcggg ctcgccaggg gattgccgtc acgcaggacg
     1981 gccagcacgg tggcgccggt ggcgcggcgg atatcgaggc catccagcgt cttgcccacg
     2041 ctcgggctgt cggccgccat ggccaggtcc tcgatgttga gcgcgtcgtc gccgcgcctg
     2101 agcgcggtct cgaagaagtc caccaccgcg gggctcagca tcaggtgggc gagccggtgc
     2161 cctccaatcg cgtacgggct caccgcccgg ttggcgccgg cgcggccgag cttggtgacg
     2221 ccggcctcgt cggcggcacg cgagacgatg tagagctcgg gggcaaggac ccgggcggac
     2281 agcaccacgt acacgttggt ggcgtcgttg cccgtggtga cgatcaggcc gcgcgcccgg
     2341 gcgatgcccg cccgcttcag caccgcgtcc tcggtggcgt ctccctggag cgccaggtat
     2401 ccccgctcgg tcgcgtgggc catcgcctgc tcgctggggt ccacgaccac gaagcgggtc
     2461 tcggcctcgt gaagctcgag ggccgcctgt cggcccacgc ggcccattcc cgccacgatc
     2521 acatgcccgg tgagctcccc gatgcgccgc tccattctgc gcctccccat gggatcggcc
     2581 aggcggacgc cgaccagata ctccatgacc acgcccagcg tgtagaacag gctgccgatg
     2641 ccggtgaacg ccagaaagat cgtgaacagg cggccggccg tggtgagggg ccggatctcg
     2701 ccatagccga tcgtggtgat cgtggtcacg gtcatgaaca gggcgtccat ccaggtcccg
     2761 tccacgtccc gccacagcca gcgatagccc agcgtgccga tgaccacgac cgcgagcacg
     2821 agcgtcagag gcagaaggag ccgtatcttc aaggaccgga cggtggggct gtcggtcgcc
     2881 atcgctgcga cgttagcaca cccatgagcg cccaccggat gccctcgccg gtttgcgccc
     2941 ccgtcttggg gtagagttct cgcagcatgg ggttcgtcgg tccgtgaaga tcctgctgac
     3001 cggcgccacc ggctatgtcg gtgggcgctt gttgaaggcg ctcgaggcgc gcgggcacga
     3061 gctccgctgc ctagcgcgca atccggcgaa cctcgcctca cgcgtggggc cgggcacgcg
     3121 cgtcgtcccg ggggacgtgc tggacgcggc gtcgctcgcg gcggccctcg ccggcatcga
     3181 gacggcctac tacctcgtcc actcgctcgg tgacgcgagc ggtttcgagg agcgcgagaa
     3241 ggcgggggcg cggaatttcg ccaccgccgc ccgcgccgcc ggcatcgccc gcatcgtcta
     3301 tctgggcggc ctcggggacg cgagggccga cctgtcgccc cacctccgca gccggcacga
     3361 ggtgggccgg atcctccgcg agtcgggtgt ccggacgatc gagctgcgcg cttcgatcgt
     3421 gatcggctcg ggcagcctct cgttcgagat gatccgcgcc ctcagcgagc acctgcccgt
     3481 gatgatcatg ccgcggtggg tctcggtgct cgcccagccg atcgccatcc aggatctcat
     3541 cgagtacctc gtccaggcgc tggacgttcc gatcgaggac agcacgatcg tcgagatcgg
     3601 cggggcggac cgactgtcct acgccgatct gatgcgcgag tacatgcggc agcgccacct
     3661 gagacgcctg atgattccgg tgccggtcct gacgccacgg ctgagcagtc tgtggcttgg
     3721 cctggtcacg ccgctgtatg cgcgggtggg ccgcaagctg ctcgagagca tgaagaaccc
     3781 cacggtcgtg cagagcgatg cggcgaaacg gctgttccgc gtggaggcgc ggggcgccgc
     3841 cgccgcgatc cgggccgcgc tcgagaacga ggatcgcgag ttcgcggaga cgcactggtt
     3901 cgactcgttg tccgcgtccg gcaccccgaa cgggagcggc gggtcccacc cggggccgcg
     3961 gttgatcgat cggcgcgagc ggcaagccga tgcgtcaccc gaagcgtgct ttgccgtcgt
     4021 cgcgcgcctc ggagggaggg gcgggtggct cgcgttcgac tggctatggc ggcttcgggg
     4081 cctcctcgat ttgctggtgg gcggcgtcgg catgcggcgc ggccgccccg agcaccgggc
     4141 gctcgtggtc ggggacacgc tcgacttctg gcgcgtcgag gcgtacgagc cgccgcggcg
     4201 gctgcgcctg ttcgcggaga tgaggctgcc ggggcgcgcg tggctcgagt tcgtcgtcga
     4261 gcccgccggc gccggctcgc gtatcacgca aaccgcgctg ttcgatccgg tcggggtcct
     4321 gggtcgcgtc tactggtatg gcgtcttccc gctccatggg ctcgtcttcg gcgcgatgat
     4381 cgaggcgatc gccaagcggg ccgcggtcgt ggagggccgg ccggaaagtc gaacggggcc
     4441 acggcagtcg ccgtgacccc gcgtcaagtt ttggtggagc tgagggggct cgaacccctg
     4501 accccaagac tgccagcctt gtgctctccc aactgagcta cagccccacc gcgtcgccca
     4561 gcttactgag cgcgcgttct tttgtcaaga ttggcgaata cttgcgacgt gctacgcgcc
     4621 ggccgcctgc ttggcggcgt tcagggccgc gtcgtagttg ggctccgtgg tgatctcctt
     4681 cacgtactca gcgtgcttga ccttgccgtc cttgcccacc acgaagaccg cccgcgcggt
     4741 gagacccgcc agcgggcctt ccttcacgag cacgccgtag ttctgggcga acgaccgctc
     4801 gcggaagtcg gacagggtct tcaccgcggt gacgccggcc gccccgcacc agcgcttctg
     4861 ggcgaagggc aggtccatgc tcaccgtcca cacttccacg ccggcgccga tcttgcccgc
     4921 ttcctggttg aaccggcgcg tctcggtgtc gcagaccggt gtatcgagcg agggcaccgc
     4981 gctcagcacg atgaccttgc ccttggcctg gctcagtttc accggctgta gcccgttgtc
     5041 caccgcggtg aaatcgggcg cgtcgctgcc cgccttgatc tcgggaccgc ccagcgtgat
     5101 cgggttgccc ttgaacatca cggcacctgc tctatcggcc atggggtgcc tccttgaatg
     5161 gaatgcggtt gtggctacgg gttccgggag cgctccacag tatacaagcg ggggttgtcg
     5221 tatcataggc acgcgccgaa gtggcggaac tggcagacgc gcacgactca aaatcgtgta
     5281 cccgtaaggg tgtgtgggtt caactcccac cttcggcacc aaccctttcc gcgaggtgag
     5341 cgagcatgac cgccgcgacg cgtagcgccc tgcacacgcc gctctgcggg cgcctcggca
     5401 tccgctatcc catctgtcag gccggcatgg ccttcgtggc gcgcgcacag ctcgcggccg
     5461 cggtctccgc ggcgggcggg ctcggcgtca tcgcggccgc gcacggaacc gccgccgatc
     5521 tgcgggaaga gatccatcgc gtgcgtgagc tcaccgacag gcccttcggc gtggatatcc
     5581 tcttcgcgag cgtgcgcacc agcgggcagg agacggagcg cttcaccgac gcggtgaagg
     5641 gctggatcga cgtcacgctc gaggagcgcg tgccggtgct gatcgcgggc ctcggcaacc
     5701 cggggccggt gaccgcggag gcgcaccgcc tcgggatcac cgtgatggcc ctctgcggca
     5761 acgtgaagca ggcgcgcgat cacgccgcca acgacgtgga cgtgatcatc gcgcaggggc
     5821 acgagggcgg cggccacacc ggccgggtgg gcggggtggt gctcacgccc gcggtggtgg
     5881 acgcggtggc gccgcgcctg gtggtggcgg cgggcggtct ggccgacggg cgcggcctgg
     5941 tcgccgcgct ggcgctgggc gcggcggggg tctggatggg cacacgcttc atcgccacgc
     6001 gagaggcgta cggccacgac aattacaagc agaagatcgc tacgatcgac gaagaaggca
     6061 cggtggtcac tcgcgcggtg tccggcaagc ccaaccgcca gatccggaac gccttcaccc
     6121 gcgagtggga gagccgccag gccgagatcc agccctttcc cgtgcagtac gagcgcgtcg
     6181 gcaagcccgc gtcgatccgc gcgcgcgagg agggcgacgt cgagcacggc agcgcggcgg
     6241 ccggccagtc ggccgggctc atctacgacg tgcgcccggt ggccgacgtg atggcgtcga
     6301 tcgtgaacga ggccgaggcg atcctgcatc ggctcggcgc ccgggcgtga cccggccgct
     6361 cgacgggcgg gtggccgtcg tcaccggcgg ctcacgcggc atcggccgca gcatcgcgct
     6421 ccggctcgcg cgcgacggcg cgcactgcgc gataacgtat cgcaaggacg cctccgcggc
     6481 ggcccgcacg gtgggtgagc tcgaggcgct gggcgtccgc gcggtcgcgg agcggctcga
     6541 gctcgccgag ccggagcagg tcgcgctcgt cttcgagcgg atcggcgcgg tgctcggccg
     6601 cgtggacgtg ctggtggcga acgcggcggc caccgcgttc cgtccggtgc tggagcagaa
     6661 gccgcacaat gtccgccgca cgttcgcgat ctcggtggac gccttcgtgg cgatggcgca
     6721 ggccgcggtc ccgctcatga agggccgacc cgggcgcatc gtgctgatct ccgggatcga
     6781 cagcttccag gccatgggcg gtcacagcgt gctgggtgcc gccaaggccg ccgccgaggg
     6841 cctggtgcgc ggcctcgcgc tggagctggg ccccctgggc gtcacggtga acgcggtgac
     6901 accgggcttc gtcaagaccg actcgtccac ctactacgtc acccaggggc tgggcctcga
     6961 ctacgagcgg gccaccgcgc gcctcctcgc ggccacgccg gtgcgccggc tcgggacgcc
     7021 cgaggacgtg gcggccctgg tggcctacct ggtctcggac gccgcgtcgt tcctgaccgg
     7081 ccagtgcgtc gtcatcgacg gtgggctcac catcacctcg cccctcagtc gcatggagga
     7141 tgggccctga tcgtgtagac tgactgccat gccctcgtcg tggttcgaac cactcccggc
     7201 ccgggcgacc taccaggcag aggcgcacgg ggagcatcgc gccgccctcg cggaggccgc
     7261 cgcggccgcc tgcgcgcgca ccgcccccac gctcctcgcg atgcagaagc ccgagggcta
     7321 ctgggtcggc gatctgctcg ccgactcgac cctcgagtcc gactacgtgc tgctgcagct
     7381 ctggctgcac ccgccgcagg acggcgagtg gaagccgcct tcctgggatc gcatccagcg
     7441 ggcgcgcaag gcgatcctgg cccagcagag gtcggacggc gggttcaaca tctatccgga
     7501 cggccccgcc gacgtcaacg ccaccatcaa ggcctacatc gcgctcaagc tcgcggggct
     7561 cggcgcccac agcgagccgc tgcagcgggc gcgcgccgcg atcctccacc tgggcggcat
     7621 ccaggaagcc aacagctacg ttcggatcaa cctgagcctc ttcggcctct acccgaagcg
     7681 ccacgtgccc acgatcccgg tggagctggc cctgcttccg ggcgggctca tctacgagat
     7741 gtcgtcctgg acgcgcgcga tcgtgatgcc gctgaccatc atccaggcga agacggacac
     7801 gcgcccggtg cccgcgggct tcacgctcga cgagctggtg gtgccgggca agagcttcaa
     7861 actgccgaag cgcgaccggc tctccgcgct gttccgccag ctcgacatcg cgctcaagat
     7921 gtgggcgacg cgcgggccgg agatgatccg gaggcccgca atccgcgagg cggagaagtg
     7981 ggtgctggac cacacgcgga acagcgacgg gctcggcgcc atctatccgt cgatgatgta
     8041 cctgatcatg gcgctggacg ccctcggcta tccacgcgac catcccgacc tcatcgaggc
     8101 catccggcag tacgaggggc tcctcaccga gacgccggag gcattctatt tccagccctg
     8161 cgtctcgccg gtgtgggaca ccgccatcgc gatgttcgcg ctcggggaga tgggcgcggt
     8221 ggcgcccgcc gcgatgcgcc ggggtgccga ctggttcatc gccaaggaga tccggcggcg
     8281 cggcgactgg tcggtgaagc ggcccgatct cgagccgtcc ggctgggcgt tcgagttcgc
     8341 caacgagcac tatcccgaca tcgacgacac cgcgatggtg ctgctggcca tgcagcacgc
     8401 gcaggcctcc gacgcggccg cccaggagcg ctgcgagcgg cgggcggtgg cgtggctcgt
     8461 gggcatgcag tcgggcgacg ggggctgggc cgccttcgac gccgacaaca actgggcctt
     8521 cctcaaccgc gtgcccttcg cggaccacaa cgcgatgctc gacccgtcgt gcccggacat
     8581 caccggccgc gtgctcgagg cgctgtgccg ccgagggttc aagcggtcgg atccttcgat
     8641 cgagaagggc gtgggcttct tgctcgagac gcaggagcgc gacgggagct ggtacgggcg
     8701 ctggggcgtg aactacctct acggcacctt cctcgccctg cgcggcctcc gggccgccca
     8761 ggatcccggc gcctcgaagg cggtgtggaa ggccgggaag tggattgcct cggtgcaaaa
     8821 cgaggacggg ggctggggcg agagctgcgc gagctacaac gagggtcgat tcgtgcccgc
     8881 gccgagcacc ccgtcccaga ccgcgtgggc gctgctcggc ctgatcgcgg ccggccaggg
     8941 cgacaccgag gtgctcgtcc gcggcgtccg gcatctcgtg gacacccaga agcccgacgg
     9001 gacctgggac gagcatctgg ccaccggcac cgggttcccc aacgtgttct acctgcgcta
     9061 cacgctctac cggacctatt tcccgctgct cgcgctcacc caggcccgcc gcgccatcga
     9121 cgaggcggag cgcaccctgc gcctcgtgca ggacgcggcc ccgttgcacc ccctgcgctg
     9181 agcggcgggc tgccgaagga ggaggggtca ggcggtctga agggcgctga atgcggcgcg
     9241 ggccaggccc ggcagctccg ggtcccgggc acccatgatc agcaccgtgt cgcccggccg
     9301 agattccgcc cggagccagt cgatcaccgc ccggtggctc gacgcgtacc cgcagcgcag
     9361 ggcggccggc aggtcgtcgg cgagcatgcg gctcgagatg tccctggcca cggtgccacc
     9421 cgcgtagaag atctccgcgt agcagaggcg gtccgcgggc cgcagcagcc gcggcatcag
     9481 ctcgcgcagc tcgggccgca ggaagcgggc ggggccgaag ccgtgcggct ggaagaccgc
     9541 gaccacgcgc gacgcgccgg cctgggccgc ggtgatcgcg gcgcgaagct tttccccgtt
     9601 gtgggcgtag tcgtccacca cgcggatgcc cgcggcggtc acgccgatca cctcgaagcg
     9661 gcgcgccacg ccggggaagc gggcgagcag cgccgcaacc gttgccgggg cgatgccgag
     9721 gtcgagcgcg atgagggccg ccgccgccgc gttctccagg ttgtggagcc cgggctgggg
     9781 tatgtcgagg acgagcggcc ccgtcggcag gtgcagggtc ccgcgcgcgc gatggggtcc
     9841 ggtgctcgtg acctcgaggg gcgtgtccgc gtctggtccc aggccgtagc cgtgcgcgcc
     9901 caccgcccgg ccgagtaggg cggcctcggg cgaggcgcta ttgaccagga gccggcccga
     9961 gcgcttggcg aaggcctcga actggggccg cagcgcgtcc acctcgccgt gatcgcggct
    10021 gatgttgtgg atgagcccga gcgcggcgtg gtagccggtc agcgtgccgt cggactcgca
    10081 ggcttccgcg gccaccggcc cgtccaccgg ccccggcagg aagcagccgc tcgtccggtc
    10141 gcccgcgagc gcggcgccgc ccagcacggt ggcggccacg cccgcctcgc gggtgagcca
    10201 gcccaccatg ccggtgatcg tgctcttgcc gctggtgcct gcgatggcga cgccgggccg
    10261 gccggcgtcc acgacctcgg ccagcagcgc ggggcgcggg acgcgctcga gtccgagcgc
    10321 gcgtgcggcg cgcacctcgg gggtctccgc ctccaccgcg gtggagtaga tgaagcggtc
    10381 caggtccgcc gtgaccgcgg agccgtcctg gggcttgacc acgatgccga gcgcggacag
    10441 gccggcggcc acctccgcgt tcttgccctg gtcgaaggag cggtcggagc cctgcacggc
    10501 gtggccgcgg gcccgcatga gccgggccag cgggctcatc ccggcgcccg cgatgccgga
    10561 gaagtggtag cgcacacttc atcctgccac gaatcgccga tcgaataatc cggtggattt
    10621 tcgcccaccc cgccagggcg aagcgcccga tggcgccgtg caagctattg atatggtgtt
    10681 ccgttgtccc tggcaccgcg cttgccacca cagggcgccc acgggaggat gccatgagca
    10741 tcgccgaagc cgaggccgtc gtcgtcaagg gtcgtttctg cggctccccc aggaccgcca
    10801 gcggcggcta catcgcgggc ctgctcgggc ggcacgtgga cggcgcggcc cgcgtgagtc
    10861 tcgattcgcc gatcccggtg gatcacgcct tcggcgccga gcggctcgcg gacgggcgcg
    10921 tggtcctctt ggatggcgcc gccgttctcg cccacgcgga gccggccacg ctgcagatcg
    10981 agccgcccac gccgccgtcg ttcgccgagg cgcagtgggc ctccgagcgc tacctgggat
    11041 tcaccgagca cgcgtccccc gagtgctttg tctgcggacc ggcgcgcgag tggggcgacg
    11101 ggctcgcgat cttccccggc ccgctgcccg gccggcacct ggtggccgcc ccctgggctc
    11161 cggatccgac gctcttcgac aagcacggca cggtgcgtcc cgagttcgtc tgggccgcgc
    11221 tcgactgtcc gagcggcttc gcgctgctcg aggctttcgg ccgccgcaag gcggtgctga
    11281 gccagctcac cgtcaagctg atccgtcccc tcgaggtcgg cgcgcggtgg attgtgatgg
    11341 gctggccctt tgcggcggat ggccgcaacc tcctcggcgc gtccgcgatc gtttcggaga
    11401 gcggcgagct gcacgcgctc gcctcggc
//
LOCUS       DASUFA010000220         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1086838, whole genome shotgun sequence.
ACCESSION   DASUFA010000220 DASUFA010000000
VERSION     DASUFA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086838"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFX87_08160"
     CDS             <1..683
                     /locus_tag="VFX87_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4994925.1"
                     /translation="TVVGQLDRRTREQNAESSKRSAALNARLDALSAEVARVSARVDE
                     LSQRVEALGRPAPPSGGAAPIPPPTRSGPAPAPSVAAVPPPARGGGSAEESYQAAYLD
                     FSKGLYPLAVSGFRDFLRRFPDSPLADSSQYWIGEAYFSMARATASQPEKARENLEQA
                     VQEFRKVVVAYPRGSKVPTALYKEALALVELKQTALAQARLQYLVEHFPQSEEAPLAK
                     ERLASLKE"
     gene            complement(716..1048)
                     /locus_tag="VFX87_08165"
     CDS             complement(716..1048)
                     /locus_tag="VFX87_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994926.1"
                     /translation="MADPEWAGFWPAVRVRIARGAPEPVREPWWLPFWKPVWGHPRVA
                     LGSLMVSSLALGLVLWPSPPVTLSAPVVVQDVSSADPNRSVMVYSSPDDDVTVIWVFN
                     PGEQDEQS"
     gene            1306..1935
                     /locus_tag="VFX87_08170"
     CDS             1306..1935
                     /locus_tag="VFX87_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX4994927.1"
                     /translation="MEEALTVHVPDGPVLEARLGPAASPRGGFVICHPHPLYGGDMDN
                     PVVIRAAEVAQAAGYATLRFNFRGAGGSKGVHDKGRDEQEDVKAAMAMLAIHLPPGSR
                     VGVMGYSFGAAMAARATRPSTPEVPLGLIAPPLGMYDLDFLQTSPGRLLLVAGTADSY
                     CPVEALHRLAALTSAEERIIEGADHFFFGKLYPLGEAIGAWLAGLPPAP"
     gene            complement(1936..2547)
                     /locus_tag="VFX87_08175"
     CDS             complement(1936..2547)
                     /locus_tag="VFX87_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX4994928.1"
                     /translation="MSGSFRNWMKVRFITGLFSTVPAIATAWLLWVFWNAIDDFFSPV
                     YTQILGRRIPGMGFLTAVIIIFIMGTIARNVVGRRVLARIERLFEHVPIFRSIYPSVK
                     QLLESFSPQQRSSFKAVVLAEHPRKGEYVFGFVTSELLLDGVEGKRAMVTVFVPTNNL
                     YLGDVIVVAKEETLPTGLTVEEGIRIILSAGTATPPRLPRERL"
     gene            2557..>3095
                     /locus_tag="VFX87_08180"
     CDS             2557..>3095
                     /locus_tag="VFX87_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994929.1"
                     /translation="MRPHGPGQVKELAGKVALVTGGAVRVGLEIARALADAGADVAVG
                     YRRSAAEARGAVRELEARGVRAAALRADVARPREARALVAGAVRRLGRLDILVNNAAL
                     FVRTPVLTTTPAQFDRLLAVNLRAPFFVSQAAARAMGRRGGRIINIADVGAVRAWAGY
                     VPYGISKAGVVMLTRGFAVA"
BASE COUNT          468 a         1079 c         1138 g          410 t
ORIGIN      
        1 agaccgtggt ggggcagctc gatcgccgca cgcgcgagca gaacgcggag agcagcaagc
       61 ggagcgcggc gctcaacgcg cggctcgacg ccctgtccgc cgaggtcgcg cgggtctccg
      121 cccgcgtcga cgagctcagc cagcgggtcg aggccctcgg ccgcccggcc ccgccgtcgg
      181 gcggcgcggc gccgatccct ccgccgacgc ggtccgggcc ggccccggcg ccgtcggtcg
      241 cggcggtacc cccgccggcc cgcgggggcg gcagcgccga ggagagctac caggccgcct
      301 atctcgattt cagcaagggg ctctatccgc tcgcggtctc gggcttccgg gatttcctgc
      361 ggcgcttccc ggactctccc ctggccgaca gctcgcagta ctggatcggg gaagcctact
      421 tcagcatggc gcgcgccacc gcgagccagc ccgagaaggc acgggagaac ctcgagcagg
      481 cggtgcagga gttccggaag gtggtcgtcg cctatccgcg cggcagcaag gtcccgaccg
      541 cgctctacaa ggaagccctc gccctcgtgg agctcaagca gaccgcgctc gcccaggcac
      601 ggctgcagta tctggtcgag cacttcccgc agtcggaaga ggcccctctc gcgaaggagc
      661 ggctggccag cctgaaggag taaagggggc gtcgcgcggg cggcgcgacc aagcgctacg
      721 actgctcgtc ctgctccccg gggttgaaga cccagatcac ggtcacgtcg tcatccgggc
      781 tcgagtacac catcacgctg cggttcggat cggcggagga gacgtcctgc accaccaccg
      841 gcgccgacag cgtcaccggc gggctgggcc agagcaccag gccgagcgcc aggctcgaga
      901 ccatgaggct gcccagggcg acccgcggat gaccccagac gggcttccag aagggcagcc
      961 accagggctc gcggaccggc tcgggggccc cgcgggcgat acgcacccgg accgcgggcc
     1021 agaagccggc ccactcggga tcggccacgt ccggtgccgt gctccggacc agcaggcgga
     1081 gccgggtaag ttgcgcgacc tcaccgccac agcgtgggca gcgggccgcg tgagacgtgg
     1141 ccgctcgctc ggcgcggggc ccgagcgctc catcgaccaa gcgctcgagg cggggccggt
     1201 tccagagaca tccgaggctc ggcatcatgg caagtatacc tcgtaggctg agacgcgggc
     1261 tggcccttga atattcccgc cggaccccct aaggtcggaa ttcctatgga agaagccctc
     1321 acggtccacg tcccggacgg ccccgtcctg gaggcccggc tcggacccgc cgcgagcccc
     1381 agggggggat tcgtcatctg ccatccccat ccgctctacg ggggcgacat ggacaatccc
     1441 gtggtcatcc gggccgccga ggtcgcgcag gcagccggct acgccacgct ccgcttcaac
     1501 tttcgcgggg cgggcggctc caagggggtc cacgacaagg ggcgggacga gcaggaggac
     1561 gtgaaggcgg cgatggcgat gctcgcgatc cacctgcccc cggggagccg ggtcggggtc
     1621 atgggctact cgttcggggc ggcgatggcg gcgcgggcca cgcggccgag cactccagag
     1681 gtgcccctcg ggctgatcgc cccgccgctc gggatgtacg atctcgactt cctccagacg
     1741 agccccggac gcctgctgct ggtggccggt accgcggatt cctactgtcc ggtcgaagcg
     1801 ctccaccggc tggccgcgct cacctcggcc gaggagcgga tcatcgaggg ggcggaccac
     1861 ttcttcttcg gcaagctcta tccgctgggt gaggcgatcg gggcctggct ggcggggttg
     1921 ccgccggccc cgtgatcaga gccgctcgcg aggcagccgg ggcggcgtag cagtgcccgc
     1981 ggagaggatg atgcgaatgc cctcctccac ggtgaggccg gtgggcagcg tctcctcctt
     2041 cgccaccacg atcacgtcgc ccagatagag gttgttggtg ggcacgaaca cggtcaccat
     2101 ggcgcgcttg ccctcgacgc cgtcgagcag cagctcggag gtgacgaagc cgaagacgta
     2161 ctcgcctttg cgagggtgct cggccaggac caccgccttg aaggagctgc gctgctgggg
     2221 ggagaacgac tccaggagct gcttcacgga ggggtagatg ctgcggaaga tcggcacgtg
     2281 ctcgaagagg cgctcgatgc gggcgaggac gcgtcggccg accacgttgc gggcgatcgt
     2341 gcccatgatg aagatgatga tgaccgcggt gaggaagccc atgccgggaa tgcgccggcc
     2401 gaggatctgg gtgtacaccg gggagaagaa gtcgtcgatc gcgttccaga acacccacag
     2461 cagccaggcc gtggcgatcg cggggacggt ggagaacagt ccggtgatga accgaacctt
     2521 catccagttg cggaaactgc ccgacatgtc agatcgatga gaccacacgg cccagggcaa
     2581 gtcaaggaac tggccggcaa ggtggcgctc gtcaccggcg gcgcggtgcg cgtcggcctc
     2641 gagatcgcgc gcgcgctcgc ggacgcgggc gccgacgtgg cggtgggcta ccgtcgctcg
     2701 gccgcggagg cgcgcggcgc ggtgcgcgag ctcgaggcgc gcggcgtgcg cgcggccgcg
     2761 ctgcgcgccg acgtggcgcg gccgcgcgag gcgcgcgcgc tcgtggcggg tgcggtgagg
     2821 cggctcggcc gtctcgacat cctggtcaac aacgcggcgc tcttcgtccg cacgccggtg
     2881 ctcaccacga cccccgccca gttcgaccgg ctcctcgccg tcaacctgcg cgcgccgttc
     2941 ttcgtgagcc aggcggccgc ccgggccatg ggccggcgcg gcgggcgcat catcaacatc
     3001 gcggacgtcg gcgcggtccg cgcctgggcc ggctacgtgc cctacggcat ctccaaggcg
     3061 ggcgtggtca tgctcacccg cggcttcgcg gtggc
//
LOCUS       DASUFA010000221         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_972804, whole genome shotgun sequence.
ACCESSION   DASUFA010000221 DASUFA010000000
VERSION     DASUFA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972804"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..603
                     /locus_tag="VFX87_08185"
     CDS             112..603
                     /locus_tag="VFX87_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX4994930.1"
                     /translation="MSLFTFAVLALLAIGSAAGMLLRKNPIHGALFLAINLSSVAALF
                     LTMHAEFLAAVQIIIYAGAIAVLFVFAIMVLIPGKEETGPDPLRRQRWLAVPVAAVFL
                     ILVALVLRSAVFAGPPKPHLPGGTDAVGRVLFTDYLFPFEVTSVLLLVAIVGVMALTK
                     RRA"
     gene            605..907
                     /gene="nuoK"
                     /locus_tag="VFX87_08190"
     CDS             605..907
                     /gene="nuoK"
                     /locus_tag="VFX87_08190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX4994931.1"
                     /translation="MVPESYYVALSAVLFTMGVLGVLIRRNPLVIFMSIELMLNAANV
                     ALVAFGQRHGTTEGQALVFFVLCVAAAEVAVGLAIIVAIFRLRRRLSVDELSTLRG"
     gene            924..1922
                     /gene="pdhA"
                     /locus_tag="VFX87_08195"
     CDS             924..1922
                     /gene="pdhA"
                     /locus_tag="VFX87_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX4994932.1"
                     /translation="MAQTKTAVVQTRLDAELARRLLTQMALIRRFEEKAAEMYALGKI
                     GGFLHLYIGQEAVAVGATSTVRVDDYAVSSYREHGHCLAKGSDPRRIMAELFGRMGGL
                     SKGKGGSMHLFDRSVNFLGGHGIVGAHLPLAAGAGFAIKYQGGDQVVLCYFGDGAVPE
                     GEFHESMNLAALWKLPVIFICENNRYAMGTAIHRALAQTDIWRFAETYGIPGEAVDGM
                     DVLAVRDVVARAVERARRDKTPALIEARTYRFRGHSMRDPAGSVYRTKEEVEREKLRD
                     PIALFSDRCLKDGILTEADIRNIEKAVNDLADEAVAFAEASPEPPPSELFTDVYKD"
     gene            1927..2907
                     /locus_tag="VFX87_08200"
     CDS             1927..2907
                     /locus_tag="VFX87_08200"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX4994933.1"
                     /translation="MAVMTYREALNLALREEMRRDPRVFVMGEEVGLYDGAYKVTQGL
                     LKEFGDKRIIDTPICESGFTGVGVGSAMLGLRPVVEMMTFNFSILALDQIVNSAAKMC
                     YMSGGQYNIPLVIRGPGGPAAQLAAQHSQSMETYFYHVPGLKVVRPSTPADAKGLLKS
                     AIRDDNPVIFIEAETLYAVKGEVPEDPDFLIPLGKAVIRREGTDVTVVAYMGMMYRAL
                     EVAEELARNGISVEIVDPRTLRPMDTETIIGSVRKTHRAVVLEAGAGFAGMGSEIAAQ
                     ITETAFDDLDAPVLRVTGENAPMPYAKNLELLKTPSKAKIAAAIRQVCNA"
     gene            2904..>4183
                     /locus_tag="VFX87_08205"
     CDS             2904..>4183
                     /locus_tag="VFX87_08205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX4994934.1"
                     /translation="MITKVFMPKLSDAMETGKVIKWLKREGDAVKGGDVIAEIETDKA
                     NVEIEAFGAGILRKIVVEEGGTVPVGDLIGVIADPTEDISSVAAAPAKPAAPAPAVAA
                     PAPAAKSAPAPAAAPAAASGPLPAMESYQSVPATTAVVPMAPAAAAPVPAGGRVKASP
                     LARKIAGQVGVDLRLIEGTGPGGRIIRRDVEAARATPRPAAAPAAAPQPAGAPRPTAV
                     PARPQFVIPPRPEAEFEDVAMSPMRAVIAKRMPMSKAPVPHFYVTSEVAMDEVWALRE
                     QLNALDGQPKISVTDMVIKAVALALLKNPGVNAQLNGQVIRVHHRAHIGIAVALDQGL
                     ITPVLRDCDVKPLARIAVESRDLADRARGGKLRAPEISGATFSISNLGMYDVEEFSAI
                     INPPEGAILAVGSVLQKPVVEDGELRVGRRMKMT"
BASE COUNT          632 a         1420 c         1497 g          634 t
ORIGIN      
        1 cgaccgccag agcatgatct acactaagga cacactcctc gcgctcgagc cggcgggccc
       61 caacggggtc ccgacgtccc cggtgccgat cgcgcctgcc cggaggccgc ggtgagcctc
      121 ttcacgttcg cggtgctggc cctgctcgcg atcggctccg cggccggcat gctgctgcgg
      181 aagaacccga tccacggcgc gctcttcctc gcgatcaacc tgagctcggt ggccgcgctg
      241 ttcctgacca tgcacgcgga gttcctggcc gcggtgcaga tcatcatcta cgcgggggcc
      301 atcgcggtcc tgttcgtctt cgcgatcatg gtgctgatcc cgggcaagga ggagacgggg
      361 cccgaccccc tgcgccgtca gcgctggctg gcggtgccgg tggccgcggt gttcctgatc
      421 ctggtcgcgc tggtgctgcg ctccgcggtg ttcgcgggcc cgcccaagcc gcacctgccg
      481 ggcgggacgg acgcggtggg gcgcgtgctg ttcacggact acctgttccc gttcgaggtc
      541 acctcggtgc tgctgctcgt cgccatcgtg ggagtcatgg cgctgaccaa gcgcagggcg
      601 taggatggtc cccgagtcct actacgtcgc gctgtccgcg gtcctgttca ccatgggcgt
      661 gctcggggtc ctgatccggc gcaacccgct cgtgatcttc atgtctatcg agctcatgct
      721 gaacgcggcg aatgtggcgc tggtggcctt cggccagcgg cacggcacga ccgaggggca
      781 ggccctggtg ttcttcgtgc tgtgcgtggc cgccgccgag gtcgcggtgg gcctcgcgat
      841 catcgtggcg atcttccggc tgcgccgccg cctctcggtg gacgagctga gcacgcttcg
      901 cggctagtca ggaggcagac cgaatggcgc agaccaagac cgcagtggtc cagacccggc
      961 tcgacgcgga gctggcgcgg cggcttttga cgcagatggc gctcatccgg cgcttcgagg
     1021 agaaggcggc cgagatgtac gccctgggca agatcggggg cttcctccac ctctatatcg
     1081 ggcaggaagc ggtcgcggtc ggcgccacct cgacggtgcg cgtcgacgac tacgcggtgt
     1141 cgtcgtaccg tgagcacggt cactgcctgg ccaaggggtc cgacccgcgc cggatcatgg
     1201 ccgagctctt cggccggatg ggcgggctga gcaagggtaa gggcggctcg atgcacctct
     1261 tcgacaggag cgtcaacttc ctgggcggcc acggcatcgt gggcgcgcac ctgccgctgg
     1321 ccgcgggggc gggcttcgcc atcaagtacc agggcggcga ccaggtcgtg ctgtgttact
     1381 tcggcgacgg ggcggtgccg gagggcgagt tccacgaatc catgaacctg gccgcgctct
     1441 ggaagctccc ggtgatcttc atctgcgaga acaaccgcta cgcgatgggc accgcgatcc
     1501 atcgcgcgct ggcccagacc gacatctggc gcttcgccga gacctacggc atccccggcg
     1561 aggcggtgga cggcatggac gtgctcgcgg tacgcgacgt ggtggcgcgc gcggtcgagc
     1621 gggcgcggcg ggacaagacg ccggccctca tcgaggcccg cacctaccgc ttccgcggcc
     1681 actccatgcg cgatccggcc ggctcggtct accggaccaa ggaagaggtc gagcgcgaga
     1741 agctgcggga ccccatcgcg ctgttcagcg accgctgcct gaaggacggc atcctcaccg
     1801 aggccgacat ccggaacatc gagaaggcgg tgaacgacct cgcggacgag gcggtggcct
     1861 tcgccgaggc ctcgcccgag ccgccgccgt cggagctgtt caccgacgtg tacaaggact
     1921 gacgccatgg cggtcatgac ctaccgggag gcgctcaatc tggccctccg cgaggagatg
     1981 cgccgggacc cgcgggtgtt cgtgatgggc gaggaggtcg gcctctacga cggcgcctac
     2041 aaggtgacgc aggggctgct caaggagttc ggcgacaagc gcatcatcga cacccccatc
     2101 tgcgaatcgg gcttcaccgg agtgggggtc ggctccgcga tgctggggct ccggccggta
     2161 gtcgagatga tgacgttcaa cttctcgatc ctcgcgctcg atcagatcgt caactcggct
     2221 gccaagatgt gctacatgtc gggcggacag tacaatatcc cgctcgtgat ccggggtccc
     2281 ggcggtccgg cggcccagct cgccgcgcag cactcgcagt ccatggagac gtacttctac
     2341 cacgtgcccg ggctcaaggt ggtgcgtccc tcgacgccgg ccgacgccaa ggggctgctg
     2401 aagtcggcca tccgggacga caatcccgtg atcttcatcg aggccgagac gctgtacgcg
     2461 gtcaagggcg aggtgcccga ggatccggat ttcctgatcc ccctcggcaa ggcggtgatc
     2521 cgccgcgagg gcaccgacgt cacggtggtg gcctacatgg gcatgatgta ccgcgccctc
     2581 gaggttgcgg aggagctggc ccgaaacggg atctcggtgg agatcgtgga cccgcggacg
     2641 ctccggccga tggacaccga gaccatcatc gggtccgtta gaaagactca tcgagcggtc
     2701 gtgctggagg cgggcgcggg cttcgcgggc atgggctccg agatcgcggc ccagatcacc
     2761 gagacggcct tcgacgatct cgacgcgccg gttctccggg tgaccggcga gaacgcgccg
     2821 atgccctacg cgaagaacct ggagctgctc aagacgccgt cgaaggccaa gatcgcggcg
     2881 gcgatccggc aggtgtgcaa cgcatgatca ccaaggtgtt catgcccaag ctgtccgacg
     2941 ccatggagac cggcaaggtc atcaagtggc tcaagaggga aggcgacgcg gtcaagggcg
     3001 gtgacgtgat cgcggagatc gagaccgaca aggccaacgt cgagatcgag gcgttcggtg
     3061 ccggcatcct ccgcaagatc gtggtggagg agggcgggac ggtgccggtg ggcgatctga
     3121 tcggcgtgat cgcggacccg acggaggaca tctcgagcgt ggcggcggct cccgcgaagc
     3181 ccgcggctcc cgcgcccgcg gtcgccgccc cggcacccgc ggccaagtcc gcgccggccc
     3241 ccgccgcggc gccggccgcc gcgagcggtc cgcttcccgc gatggagagc taccagtccg
     3301 tgcccgctac caccgcggtg gtgccgatgg cgcccgccgc cgcggcgccg gtccccgcgg
     3361 gcggccgggt gaaggcctcg ccgctcgcca ggaagatcgc gggccaggtc ggcgtggacc
     3421 tgcggctgat cgagggcacg ggccccggcg ggcggatcat ccgccgcgac gtggaggccg
     3481 cccgggcgac gccgcggccc gccgcggcgc ccgcggccgc cccccagccg gcgggcgctc
     3541 cgcgcccgac cgccgtcccc gcgcggccgc agttcgtgat cccgcctcgg cccgaggccg
     3601 agttcgagga cgtggcgatg tcgccgatgc gcgcggtcat cgccaagcgc atgccgatgt
     3661 cgaaggcacc ggtcccgcac ttctacgtga cctcggaagt cgcgatggac gaggtgtggg
     3721 cgctgcgcga gcagctcaac gccctcgacg gacagcccaa gatctcggtg accgacatgg
     3781 tcatcaaggc ggtggcgctg gccctgctca agaacccggg cgtcaatgcc cagctcaacg
     3841 gccaggtcat ccgggtgcac caccgggccc acatcggcat cgcggtggcg ctcgaccagg
     3901 gcctcatcac tccggtcctg cgtgactgcg acgtgaagcc gctggcccgc atcgcggtcg
     3961 agtcgcggga cctggccgac cgagcccgtg ggggtaaact tcgcgcgccg gaaatatccg
     4021 gggccacctt ttccatctcg aacctgggca tgtacgacgt cgaggagttc tcggccatca
     4081 tcaacccgcc cgagggcgcg atcctggcgg tgggctcggt gctgcagaag ccggtggtgg
     4141 aggacggcga gctccgcgtg ggccgccgca tgaagatgac gat
//
LOCUS       DASUFA010000222         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_56038, whole genome shotgun sequence.
ACCESSION   DASUFA010000222 DASUFA010000000
VERSION     DASUFA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56038"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFX87_08210"
     CDS             complement(<1..1175)
                     /locus_tag="VFX87_08210"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4994935.1"
                     /translation="MADPLRPPLHGVRVLELAHLIAGPICGMYLADMGADVVKVESRE
                     APDAGRSVYPRAMRNGEGVLHLTVNRNKRGICLDVKQPEGREAFRRLAAWADVIIEGF
                     RGGVAERLGIDYDSVKGANPRLIYCSISAFGPDGPWREKPGLDSLAQALGGLMAITGE
                     PDGGPVLCGAPVADTLGGMLAIQGILTALIARAASGQGQRIDASLLNGMLFAHTARLS
                     VFHETGEALPRYGSGHPEIVPYQAFHAADGWIFVAAWVDRLWLPFCKAVGMDALGSDS
                     RFATRKDRLAHRGALGAILAPVFRARPVAHWMDVLEQADVLCAPVNDYPMLVRHPQVV
                     ATGFITEQQHPRAGTFKTVATPVKLEKTPGTIRTPAPRLGEHSREVLAEAGFTTAEI"
     gene            complement(1198..2001)
                     /locus_tag="VFX87_08215"
     CDS             complement(1198..2001)
                     /locus_tag="VFX87_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX4994936.1"
                     /translation="MIGALRTAAANFRARRGMFLASGLAFNFMVGLIPLLFFFVSVAG
                     FVLSRRTVMDAVLNQLSAIVPVYKNELHEALAAIIRRRRLSGLLGTAVLLLFASQLFN
                     SIRVVLSDIFGFRGGPGFLRGMLRDMVMVVAMGLIFLGSLVISDVFGWLKILLFQTMG
                     IPSQWVRSWSLALALTLSTAFFFVPDRYFPHRRVPVGAALAGALLAAVLWEAAKQLFR
                     WYILSFGVYDQVYGPLGVLVALAMFAYYSGVVFILGAEFASALMRTSRG"
     gene            complement(2033..2824)
                     /locus_tag="VFX87_08220"
     CDS             complement(2033..2824)
                     /locus_tag="VFX87_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4994937.1"
                     /translation="MDLGIKGKTALVVAASKGMGKASALGLAAEGARVVMCARGEAAL
                     KDAASEVKRQTGAEVLALTADASRAADISKVVAEANRAFGGVDILVANVGGPPPGPFE
                     SMTDEHWTKAFEQVHLSTVRFIREVLPHMKKSRWGRILAIQSSSVKQPVDGLVLSNGI
                     RPGIAGLFKTLAGDLAKDNITVNLVLPGRIMTDRFIEHQTDRAKRGGITMEQQMALSS
                     ADIPMGRIGTPEEFAAMVVFLASARASYVTGTAVQVDGGLIRSIV"
     gene            complement(2852..3760)
                     /locus_tag="VFX87_08225"
     CDS             complement(2852..3760)
                     /locus_tag="VFX87_08225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX4994938.1"
                     /translation="MDASVELVKHLLGSVVHIHAEVPASHPSTRIMGDERMGTGTVVD
                     PAGLILTVNYVVMGAETIQVTLGKGRALRAEIVAQDFEIGLALLRVKRQGLPAVPIAD
                     SETLTRGEPVFAIGSTGPRERRVAGGLVTHLGEFEAYWEYLLERGIVSSASNPGFGGG
                     PLFTLTGQMVGVVSLNLNEIARCSLAIPGEYYRRNQQEFMRFGRVVSRPQRAWLGVFA
                     HVLEEGVVVAGLVPNGPGARSGIQEGDVIVSLDAQDVPTRKEFYLSLWKHAPGEKITL
                     DVMRDNEVRRLSVTGGDRADFYKQTS"
     gene            complement(3833..>4234)
                     /locus_tag="VFX87_08230"
     CDS             complement(3833..>4234)
                     /locus_tag="VFX87_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4994939.1"
                     /translation="GPSLERARRRGVELPAPGFAAGVGVYPGGCFSLVKEQVVRPLLV
                     LIGEADDWTPAAKCREMVEAMRGRGADATIVVYPGAYHYFDVEGQRLEVLAQVENDNR
                     PGGFGATVSYQATAAADARRRIEDFLARHLK"
BASE COUNT          672 a         1489 c         1494 g          579 t
ORIGIN      
        1 tcgatctccg cggtcgtgaa gccggcctcg gcgagcacct cccggctgtg ctcgcccagc
       61 cgcggggccg gggtccggat ggtgcccggc gtcttctcga gcttcaccgg ggtggccacc
      121 gtcttgaacg tgccggcgcg cggatgctgc tgctcggtga tgaagccggt ggccaccacc
      181 tgcgggtggc ggaccagcat cgggtagtcg ttgaccggcg cgcagagcac gtcggcctgc
      241 tcgagcacgt ccatccagtg ggcgaccggg cgcgcgcgga agaccggggc gagaatcgcc
      301 cccagcgccc cgcggtgggc gagacgatcc ttgcgcgtgg cgaagcggga atcggatccg
      361 agcgcgtcca tgccgaccgc cttgcagaag ggcagccaca ggcgatccac ccacgcggcc
      421 acgaagatcc agccgtcggc ggcgtggaag gcctggtagg gcacgatctc gggatggccg
      481 ctgccgtagc gcggcagcgc ctcgcccgtc tcgtggaaga cggagagacg cgcggtgtgg
      541 gcgaagagca tgccgttgag cagcgaggcg tctattcgtt ggccttgccc gctggcggcg
      601 cgcgcgatca gggcggtgag gatgccctgg atcgcgagca tgccgcccag ggtgtcggcc
      661 accggagccc cgcacaggac cgggccgccg tcgggctcgc cggtgatggc catgaggccc
      721 cccagggcct gggccagcga gtcgaggccg ggcttctcgc gccagggacc gtcgggcccg
      781 aaggcggaga tcgagcagta gatgaggcgc gggttggcgc ccttgaccga gtcgtagtcg
      841 atgccgagcc gctcggccac gccgccgcgg aagccctcga tgatcacgtc ggcccacgcg
      901 gcgagccggc gaaaggcctc ccggccctcc ggctgcttga cgtcgagaca gatcccgcgc
      961 ttgttgcggt tgaccgtgag gtgcagcacg ccctcgccgt tgcgcatcgc gcgaggatag
     1021 accgaccgtc ctgcgtcggg cgcctcccgc gactccacct tcaccacgtc ggcgcccatg
     1081 tcggcgaggt acatgccgca gatgggcccc gcgatcaggt gggccagctc gaggacccgc
     1141 acgccgtgaa ggggcgggcg cagagggtcg gccatgaccg catcacccta gcacagatca
     1201 gccgcggctg gtccgcatca gggcggacgc gaactccgcg cccaggatga agacgacgcc
     1261 cgagtagtag gcgaacatcg ccagcgccac caggaccccg agggggccgt agacctgatc
     1321 gtagaccccg aaggagagga tgtaccagcg gaagagctgc ttggccgcct cccatagcac
     1381 cgcggcgagg agcgcgcccg cgagcgccgc gcccaccggg acccggcgat gcgggaagta
     1441 gcggtccggc acgaagaaga aggcggtgct cagcgtcagg gccagcgcga gcgaccagga
     1501 gcgcacccac tgggacggga tccccatggt ctggaagagc aggatcttga gccacccgaa
     1561 gacgtcgctg atgacgaggc tgccgaggaa gatcaagccc atcgccacca ccatcaccat
     1621 gtcccgcagc atgccgcgga ggaagccggg cccgccccgg aagccgaaga tgtcgctcag
     1681 gaccacccgg atcgagttga agagctggct ggcgaagagg agcagcaccg cggtaccgag
     1741 gagcccggag aggcgccgcc gccggatgat ggcggccagg gcttcgtgga gctcgttctt
     1801 gtacaccggg acgatcgcgg agagctggtt gaggacggcg tccatcacgg tgcgccggct
     1861 cagcacgaag cccgccaccg acacgaagaa gaagagcagc gggatgagcc ccaccatgaa
     1921 gttgaacgcg agccccgagg ccaggaacat cccgcggcgg gcgcggaagt tcgcggccgc
     1981 cgttctgagc gcgccgatca cgagcccgcg ctccccaccc gtctcgcccg agctacacga
     2041 tgctccggat caggccgccg tccacctgca ccgcggtgcc cgtcacgtag gacgcgcgcg
     2101 cggaggccag gaagaccacc atcgcggcga actcctccgg cgtgccgatc cgtcccatcg
     2161 ggatgtcggc ggaggacagc gccatctgct gctccatcgt gatgccgccg cgcttggccc
     2221 gatcggtctg gtgctcgatg aagcggtcgg tcatgatgcg gccgggcagc accaggttga
     2281 ccgtgatgtt gtccttggcg agatcgccgg ccagggtctt gaaaaggccc gcgatgccgg
     2341 gccggatgcc gttggagagg accagcccgt ccaccggctg cttcaccgag ctggactgga
     2401 tggccaggat gcggccccag cgcgacttct tcatgtgggg taggacttct cggatgaacc
     2461 gcacggtcga gaggtggacc tgctcgaagg ccttcgtcca gtgctcgtcc gtcatgctct
     2521 cgaagggccc ggggggcggg ccgcccacgt tggccaccag gatgtccacg ccgccgaaag
     2581 cccggttggc ctccgccacg accttcgaga tgtcggcggc gcggctcgcg tcggcggtca
     2641 gcgccagcac ctccgcgccg gtctgccgct tcacctcgga cgccgcgtcc ttcaacgccg
     2701 cctcgccgcg cgcgcacatc acgacccgcg cgccttccgc ggccaggccc agcgcgctcg
     2761 ccttgcccat gcccttgctc gccgccacca cgagggcggt cttgcccttg atgcccagat
     2821 ccataggggc ccctttgtcg gcctgcgtga atcaggatgt ctgcttgtag aagtcggccc
     2881 ggtcgccgcc ggtcacggac agccggcgca cctcgttgtc tcgcatgacg tcgagggtga
     2941 tcttctcgcc cggcgcgtgc ttccagagcg agaggtagaa ctccttgcgc gtcggcacgt
     3001 cctgggcgtc cagggatacg atgacgtcgc cctcctggat cccggagcgc gcgccgggcc
     3061 cgttcggcac caggcccgcc acgaccactc cctcttccag cacgtgggcg aagacgccca
     3121 gccaggcccg ctgcgggcgg ctgaccacgc ggccgaagcg catgaactcc tgctggttgc
     3181 ggcggtagta ctcgcccggg atggcgagcg agcagcgcgc gatctcgttc aggttcagcg
     3241 agactacgcc caccatctgg ccggtcagcg tgaagagcgg gccgcccccg aagcccggat
     3301 tggaagcgct cgagacgatc ccgcgttcga gcaggtactc ccagtaggcc tcgaactccc
     3361 cgaggtgggt cacgagcccg ccggccacgc gccgctcgcg gggcccggtg gagccgatcg
     3421 cgaagacggg ctccccgcgc gtgagcgtct cggaatccgc gatggggacg gccggcagcc
     3481 cctgccgctt cacgcggagc agggccagcc cgatctcgaa atcctgggcg acgatctcgg
     3541 cccgcagcgc ccgtcccttc cccagggtga cctggatggt ctcggcgccc atgaccacgt
     3601 aattcacggt gaggatgagg ccggcgggat ccaccaccgt cccggtgccc atccgctcgt
     3661 cacccatgat gcgtgtggag gggtggctcg cggggacctc ggcgtggatg tgcaccaccg
     3721 agccgagcag atgcttgacg agctcgaccg acgcgtccat gaattgagtg cgggctcacg
     3781 ctagcacgcg cggcccggcg cttgcaaacg cgagagcgta cgaaccgacc ggctatttca
     3841 ggtgccgcgc caggaagtcc tcgatccgtc ggcgggcgtc cgccgccgcc gtcgcctggt
     3901 aagagaccgt ggcgccgaag ccgcccgggc ggttgtcgtt ctccacctgg gccagcacct
     3961 cgaggcgctg tccctcgacg tcgaagtagt ggtaggcgcc cggatagacg acgatggtgg
     4021 cgtcggcgcc gcgcccgcgc atggcctcga ccatctcacg gcacttggcc gccggcgtcc
     4081 agtcgtcggc ctccccgatg agcaccagca gcggacggac cacctgttcc ttgaccagag
     4141 agaagcagcc gcccgggtag acgccgacgc ccgccgcgaa accgggggcg ggcagctcga
     4201 cgccgcgccg gcgggcgcgc tcgaggctcg ggcc
//
LOCUS       DASUFA010000223         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1293853, whole genome shotgun sequence.
ACCESSION   DASUFA010000223 DASUFA010000000
VERSION     DASUFA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293853"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1191)
                     /locus_tag="VFX87_08235"
     CDS             complement(106..1191)
                     /locus_tag="VFX87_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX4994940.1"
                     /translation="MRITHVTTRVLSTPADNPLVVGLPAPTDTREFVLLELGTDQGLV
                     GIGLTFFGGALTPALRAAVDSLGRLAIGEDPRQTEAIVAKCRRAAGGSGPGGIFSLAL
                     SAVDMACWDLAGKAAGRSVCALLGGLRDRAPTYASGALMRPHPVDYLAKAGPRLRDMG
                     FRQMKMQCGSEPTIAASIERVRVMREAIGPDVDLMCDINQLWSVHHAIEVGRRIEPYQ
                     LYWLEDPTAHDDYPGLARIADALATPIAAGEYHYGIVPFRHLLEARSIDIVMIDLLRV
                     GGITQWMKVAGMAEAFNLPVVSHLIPEIHVHLIAAIPNGLTVEYMPWTLRLYEETPKI
                     EAGQLVVPDKPGLGLAFDQAAIKRYQI"
     gene            complement(1188..2114)
                     /locus_tag="VFX87_08240"
     CDS             complement(1188..2114)
                     /locus_tag="VFX87_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4994941.1"
                     /translation="MKLLLFDDFKLGVLRGDGVVDVSDTVKDIPHTGPGDLMSGLIAR
                     FADYRKKIEDAAGRGKPQPVAKVRIRPPLPKPTNIVCMAVNYMEDGTRTEPAPINAFM
                     KSPSAVIGPNETMVLPDVPATIFEGEAEMALVIGKRATAVPAARAMEYVFAYCNFIDG
                     SARGLLPPGNTFYQMKSRDTFAPMGPYLVTADEIADPHKLAIRLSVNGTVKQAFNTSD
                     MAHKIPRCIEWVTSIHTLEPGDVLATGTNHRGLSGFQDGDRIELETEGLGTLHFNVRD
                     DLKRTWSRETRLDRQEKGQEGTTPQLTGKHKK"
     gene            complement(2156..3115)
                     /locus_tag="VFX87_08245"
     CDS             complement(2156..3115)
                     /locus_tag="VFX87_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX4994942.1"
                     /translation="MRRSLLALLAALLLVPPAASAQVAVQAYALPPGGGPHDVAVGAD
                     GIVWYTAQRSGHLGRLDPAGGKVDLISLGPAAAPHGVIVGPDGAPWVTDSGTNSIVRV
                     DPRTREVKRWSLPEARGYVNLNTATFDRRGRIWFTGQNGVYGRLDPATGAMQVWDAPR
                     GRGAYGIATTPDGDVYYASLAGSHIARVDVDTGTATVIEPPTKDQGARRVWSDSRSRI
                     WVSEWNSGNVSRYDPKAKEWKTWKLPGASPRAYAVYVDDKDKVWLSEWSANAMVRFDP
                     ETEKFEAFPSDRRSANVRQILGRPGEVWAPESGADRLVVYRAK"
     gene            3273..4394
                     /locus_tag="VFX87_08250"
     CDS             3273..4394
                     /locus_tag="VFX87_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4994943.1"
                     /translation="MKGRVAVLKAYGGEFELREYPVPDPEPGAVLIKLTRAGVCGSDL
                     HIWRGEMKETYGALPKDLTFGHEMCGKVERLGAGVTTDSMGRPLREGDRVTYCYFFPC
                     GRCPVCLHDEMGSCPRKARANRVAGTAPYFNNAYGDYYYLRPGHFIFKVPDEIPEDVA
                     TPINCALSQVLYGLRRAGFRAGHSLVVQGAGGLGINAVAVARDMGADTIIVIDRLAER
                     LALAKSFGADHTVNATEIASADERVDAVKALTDGFGAHVVADFVGYADVIPEGLRMLR
                     GGGCYLEVGSISPGNVFSYDATALVRANARLVATSNYSPWALEESLSFMKRNLKRFPF
                     DKVISHVFPLERISEAFAQSDWAQRGGDPAGVSRSAISM"
     gene            complement(4387..>5116)
                     /locus_tag="VFX87_08255"
     CDS             complement(4387..>5116)
                     /locus_tag="VFX87_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX4994944.1"
                     /translation="SSRRLPIISIGGYYREGKTLADIGREMESYRQAGMAGCKFKVGG
                     LAPEADAERVAAARRAAGPDFVLAVDANRGWSVEDAVRFARLVEPLDIRWFEEPCHWH
                     DDARMMAEVRRATRIPVTAGQSEITSHAVRRLLDAGAVDLVNFDASEGGGVTEWRRAA
                     ALCEAAGVKMAHHEEPQIAGHLLAAVPHGTYVECFADPERDPAWQTLWANRPAVKDGT
                     FEVSDAPGFGLVLDEALIRRYRST"
BASE COUNT          716 a         1763 c         1832 g          805 t
ORIGIN      
        1 gatgcccgag cctatcaggg cagccgctgt tcgagcgccg gctccgccgg cgcaatcttg
       61 ctgttggggg aggtctcgga gggggccacc tgggccccct ccgatctaga tctggtagcg
      121 cttgatcgcc gcctgatcga aggcgagccc gagcccgggc ttgtccggca ccacgagctg
      181 gccggcctcg atcttcgggg tctcctcgta gagccggagc gtccacggca tgtactcgac
      241 ggtgagcccg ttggggatcg ccgcgatcag gtgcacgtgg atctccggga tcaggtggct
      301 caccaccggc aggttgaagg cctcggccat gccggccacc ttcatccact gggtgatgcc
      361 gcccacacgc aggagatcga tcatcacgat gtcgatcgag cgcgcctcca gcaggtggcg
      421 gaacggcacg atgccgtagt ggtattcgcc cgccgcgatc ggggtggcga gcgcgtccgc
      481 gatgcgcgcc aggcccgggt agtcgtcgtg ggcggtcggg tcctccagcc agtagagctg
      541 atacggctcg atgcgccggc cgacctcgat cgcgtgatgg acgctccaga gctggttgat
      601 gtcgcacatc aggtccacgt cggggccgat ggcctcgcgc atgacccgca cccgctcgat
      661 cgaggccgcg atggtcggct cgctcccgca ctgcatcttc atctggcgga agcccatgtc
      721 gcgcaggcgt ggccccgcct tggccaggta gtccaccgga tgcgggcgca tcagcgcgcc
      781 gctggcgtac gtcggcgcgc ggtcgcgcag gcccccgagc agcgcgcaca ccgagcggcc
      841 ggccgccttg ccggccagat cccagcaggc catgtccacc gccgagagcg cgagcgagaa
      901 gatgccgccc ggccccgagc cgcccgcggc gcgccggcac ttggcgacga tggcctcggt
      961 ctggcgcgga tcctccccga ttgccaagcg gcccaggcta tcgacggcgg cgcggagggc
     1021 cggcgtgagc gcgccgccga agaaggtgag gccgatgccg accagccctt ggtcggtgcc
     1081 gagctcgagg agcacgaact cgcgcgtgtc ggtgggcgcg ggcaggccca ctaccagcgg
     1141 attgtcggcc ggggtggaga ggacccgcgt ggtgacgtgg gtgatcctca tttcttgtgc
     1201 ttgccggtga gctggggcgt ggtgccctct tgccctttct cctgccggtc gagccgcgtc
     1261 tcgcgcgacc acgtgcgctt gaggtcgtca cgtacgttga agtggagcgt ccccaggcct
     1321 tcggtctcga gctcgatgcg gtccccgtcc tggaagccgg agaggccgcg gtggttggtg
     1381 ccggtggcca gcacgtcgcc cggctccagc gtgtggatgc tcgtgaccca ctcgatgcag
     1441 cgcgggatct tgtgggccat gtcgctggtg ttgaacgcct gcttgacggt gccgttcacc
     1501 gagaggcgga tcgcgagctt gtgcgggtcc gcgatctcgt ccgcggtcac caggtagggc
     1561 cccatgggcg cgaaggtgtc gcgcgacttc atctggtaga aggtgttgcc gggaggcagg
     1621 aggccgcggg ccgagccgtc gatgaagttg cagtaggcga agacgtattc catcgcccga
     1681 gcggccggca ctgcggtggc gcgcttgccg atcaccagcg ccatctcggc ctcgccctcg
     1741 aagatggtgg cgggcacgtc gggcagcacc atggtctcgt tggggccgat caccgcgctg
     1801 ggcgacttca tgaaggcgtt gatcggcgcc ggctcggtgc gggtgccgtc ctccatgtag
     1861 ttcacggcca tgcagacgat gttggtcggc ttgggcaggg gcggccggat ccggaccttg
     1921 gccaccggct gaggcttgcc gcggccggcc gcgtcctcga tcttcttccg gtagtccgcg
     1981 aagcgggcga tcaacccgct catcaggtcg cccggcccgg tatgcgggat gtccttcacg
     2041 gtgtccgaga cgtccaccac cccatcgcca cggagcacgc caagcttgaa atcgtcgaag
     2101 agtaggagct tcacggtgtc ctcctgaggg ttgagccggg tatcgaacca gtccttcact
     2161 tcgcccggta cacgacgagc cgatccgctc cggactcggg cgcccacacc tctcccggcc
     2221 ggccgagaat ctggcgcacg tttgcgctcc ggcgatcgct ggggaacgct tcgaacttct
     2281 cggtctccgg atcgaagcgg accatcgcgt tggcgctcca ctcgctcagc cacaccttgt
     2341 ccttgtcgtc gacgtagacg gcgtaggcgc gcgggctggc cccgggcagc ttccaggtct
     2401 tccactcctt tgccttcggg tcgtaacggc tcacgttgcc ggagttccac tcgctgaccc
     2461 agatccggct ccgggagtcg gaccagacgc gccgcgcgcc ctggtccttg gtgggcggct
     2521 cgatcacggt ggcggtcccg gtgtccacat ccacccgtgc gatgtggctg cccgcgagcg
     2581 aagcgtagta gacgtcgccg tccggggtgg tcgcgatgcc ataggccccg cgtcctctcg
     2641 gagcgtccca gacctgcatg gcgcccgtgg cgggatcgag acgcccgtac accccgttct
     2701 gcccggtgaa ccagatgcgg ccccgcctgt cgaaggtcgc ggtgttgagg ttcacgtagc
     2761 cgcgggcctc gggcagtgac caccgcttca cctcccgcgt ccgaggatcc actctgacga
     2821 tggagttggt accgctgtcc gtgacccacg gcgcgccgtc gggtccgacg atcacgccgt
     2881 gcggcgccgc cgcgggcccg agggagatga gatccacctt gccgcccgcg gggtcgaggc
     2941 gtccgagatg gccgctgcgc tgagcggtgt accagacgat gccgtcggcg ccaaccgcca
     3001 cgtcgtgagg cccgcccccg ggcgggagag cataggcctg cacggcgacc tgggccgagg
     3061 cggcgggcgg aaccaggagc agggcggcga ggagcgcgag cagcgagcga cgcatggcca
     3121 cctccatcgg aagcggggca gtgcgaaagg cccgcccatg gtaccccagc ttcggccgct
     3181 ccggccaccg gcgatgcgcg ggtccacgag gttgacaacg gggcccgcga gcggcgaccc
     3241 tctcgctgaa tccattccag ggaggagccc caatgaaggg acgggtggcg gttctcaagg
     3301 cctacggcgg cgagttcgag ctgcgcgagt acccggtgcc ggacccggag cccggcgcgg
     3361 tcctgatcaa gctcacccgc gcgggggtgt gcggctccga tctgcacatc tggcggggcg
     3421 agatgaagga gacgtacggg gcgctaccca aggacctcac cttcggccac gagatgtgcg
     3481 gcaaggtgga gcgcctcggc gccggcgtca ccaccgactc gatgggccga ccgctgcgcg
     3541 agggcgaccg ggtgacgtac tgctacttct tcccgtgtgg ccgatgcccg gtgtgcctcc
     3601 acgacgagat ggggagctgc ccgcgcaagg cccgcgcgaa ccgggtggcc ggcaccgccc
     3661 cctacttcaa caacgcctac ggtgactact actacctccg gccgggtcac ttcatcttca
     3721 aggtgcccga cgagatcccg gaggacgtgg ccaccccgat caactgcgcg ctctcccagg
     3781 tcctctacgg gctgcgcagg gccgggttcc gcgcgggcca ttcgctggtg gtgcaggggg
     3841 cgggcgggct cggcatcaac gcggtggcgg tggcgcggga catgggcgcc gacaccatca
     3901 tcgtgatcga ccggctggcc gagcggctgg cgctcgcgaa gtcgttcggc gccgaccata
     3961 ccgtgaatgc gacggagatc gcctccgccg acgagcgagt ggacgcggtg aaggcgctga
     4021 ccgacggctt cggggcccac gtggtggccg acttcgtcgg ttacgccgac gtgatcccgg
     4081 agggcctgcg catgctgcgg ggcggcggct gctatctcga ggtgggcagc atctcgcccg
     4141 gcaacgtctt ctcctacgac gccaccgcgc tggtgcgcgc caacgcgcgg ctggtggcga
     4201 cgtcgaacta ctccccgtgg gccctcgagg agtcgctctc gttcatgaag cgcaacctca
     4261 agcgcttccc gttcgacaag gtgatctccc acgtcttccc gctcgagcgc atctcggagg
     4321 ctttcgccca gagcgactgg gcccagcgcg gcggcgaccc cgcaggggtc tcccgctccg
     4381 ccatctctat gtagagcggt agcggcggat cagggcctcg tcgaggacca ggccgaagcc
     4441 gggggcgtcg gagacctcga acgtgccatc cttgacggcg gggcggttgg cccagagggt
     4501 ttgccaggcg gggtcgcgct ccgggtcggc gaagcattcc acgtaggtgc cgtggggcac
     4561 cgcggccagc aggtggccgg cgatctgggg ctcctcgtgg tgggccatct tcacgccggc
     4621 ggcctcgcag agcgcggcgg cgcggcgcca ctcggtcacg ccgccgccct cggaggcgtc
     4681 gaagttcacc aggtcgaccg cacccgcgtc cagcagccgt cgcaccgcgt ggctcgtgat
     4741 ctcggactgg ccggcggtga ccgggatgcg cgtcgcgcgc cgcacctccg ccatcatgcg
     4801 ggcgtcgtcg tgccagtggc agggctcctc gaaccagcgg atgtcgagcg gctcgacgag
     4861 gcgcgcgaac cgcaccgcgt cctccaccga ccagccgcgg ttggcgtcca ccgccaggac
     4921 gaagtcggga cccgcggcgc ggcgggccgc cgcgacccgc tcggcgtcgg cctccggggc
     4981 gaggccgccc accttgaact tgcagcccgc catgccggcc tggcggtagc tctccatctc
     5041 gcggccgatg tcggcgaggg tcttgccctc gcgatagtag ccgccgatcg agatgatggg
     5101 cagccgccgg gacgac
//
LOCUS       DASUFA010000224         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1293954, whole genome shotgun sequence.
ACCESSION   DASUFA010000224 DASUFA010000000
VERSION     DASUFA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293954"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFX87_08260"
     CDS             complement(<1..219)
                     /locus_tag="VFX87_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4994945.1"
                     /translation="MRHTRLGRTGLSVSRLCLGTMTFGLQCDETASRAILDRAAAGGV
                     TFLDTADVYPLGGGLETVGRTEEILGGWL"
     gene            294..1283
                     /locus_tag="VFX87_08265"
     CDS             294..1283
                     /locus_tag="VFX87_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4994946.1"
                     /translation="MEYRTLGRTGLKVSALAFGCGDVGGLMVRGTPAERERGVARALE
                     HGINYLDTAPAYGSGESEKNLGQVLRVLKPAAIVGTKWRLVAADLADVPAAVARSVET
                     SLGRLGLERVDLLHLHNLIGRVGEERPLGVARVLEAVVPAVRRLQEQGKVRFFGVTAS
                     GETGALHRALSSGAIDTAQVVFNLLNPTGAYEVPAGFPAQDYDRLLLLAREHGVGTIG
                     IRAVAGGALSGQVARHPTAIPSVSPIASGPDYATDVARARALEPLVAQGHAGSLIEAA
                     LRFTITGDAMSTVQIGCSDLAQLDFAAAAVNKGPLSPAALSTLTSIWSQLATR"
     gene            complement(1343..2413)
                     /locus_tag="VFX87_08270"
     CDS             complement(1343..2413)
                     /locus_tag="VFX87_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX4994947.1"
                     /translation="MAAGARKFKIVVQKPSGGITYDLADSAYSIEREALDPIGAEIVE
                     VPAKTEEEFIAAAQDADAVIARNRRITAAIIKGLKNCKVIGLGSVGADTVDVDAATEA
                     GIVVTNVPDVFIDEVADHTMAMFLAAHRRLRLMHQLTVDNKWTEGRPYFTNIPRLYGQ
                     TIGLISFGNVAKAVARRCHAFGLRVIAYDPFLAELEMTAVGVEPVTSLVELCQRSDFL
                     SMHAPLNSETRHMMGEREFRAMKKSAIFINNGRGPTVNEAALAEALRQGWIAGAALDV
                     FEVEPVDPNNALLHMDNVLVSPHIASATARMAPETRRRLGRELATVLQGKWPRSAVNP
                     GVLPKTSLIRWQPYPMGRGPNR"
     gene            2524..3318
                     /locus_tag="VFX87_08275"
     CDS             2524..3318
                     /locus_tag="VFX87_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994948.1"
                     /translation="MLVGAFAVAHTPQLLIRPETEDRDLVLRVHAAMGRVKRRLQELR
                     PDAIAIVAGDHVEAFFLDAVPALAVYVGRECAGTFGPYRYRLDIHEPLARAVVEQGIE
                     RGFDLLYTQDAKLDYAFYVPLHFVMPEPARPIVPLHVNVYLPPQPTPARCHAWGAALG
                     EILRARPERVALIASGGMSHFPGTDRYASPEFDYDRALLDLLRDGRGRDLARVTGEEL
                     DKRGNIELRTWITLLGAVGDVRAEVYCYEPSWHHGNAVVEWPLETA"
     gene            3315..3593
                     /locus_tag="VFX87_08280"
     CDS             3315..3593
                     /locus_tag="VFX87_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994949.1"
                     /translation="MSEGAHYRFPPSEAYPLNRCLYALKSDPAFIERFLADPVAATLE
                     MGLTEGERADLRRMDRDALVARGAHPYLVFMAGLRLKIAAEPGALEYF"
     gene            complement(3590..3979)
                     /locus_tag="VFX87_08285"
     CDS             complement(3590..3979)
                     /locus_tag="VFX87_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994950.1"
                     /translation="MTRFTTMLGAATLMISTAMVAESALAPRGFAQGRPHPLQPSGPR
                     LDRLVRAPGMIEGTLTRVDGRSESVDVSIFLGLLGKTLEVVPDTLIQVDGRDARFADL
                     QEGAKVKAFYEERGAKLVATRLEVSTS"
BASE COUNT          573 a         1521 c         1463 g          618 t
ORIGIN      
        1 gagccagccg ccgaggatct cctcggtgcg gcccaccgtc tcgaggccgc cgcccagcgg
       61 gtagacgtcg gcggtatcga ggaaggtgac gccccccgcg gccgcgcggt cgaggatggc
      121 gcgggacgcc gtctcgtcgc actgcaggcc gaaggtcatg gtgcccaggc agagacgcga
      181 gacggacagg ccggtgcggc cgaggcgcgt gtggcgcatg cagatcagag agtaaacgac
      241 cctcccgcgg cgggcaagcg ggccgcccgc ggaccgtggt ataaccagcg gccatggaat
      301 accgaacgct gggacgaacg ggattgaagg tctccgcgct ggccttcggc tgcggcgacg
      361 tgggcgggct catggtgcgc ggcacgccgg ccgagcggga gcgcggggtc gcgcgcgcgc
      421 tcgagcacgg aatcaactat ctcgacaccg ccccggccta cgggtcgggc gagtcggaga
      481 agaacctcgg ccaggtgctg cgcgtgctca agcccgccgc gatcgtgggc accaagtggc
      541 gactcgtcgc cgccgacctc gccgacgtgc ccgcggcggt cgcgcgatcg gtggagacga
      601 gcctcggccg gctggggctc gagcgggtgg acctgctgca cctgcacaac ctgattgggc
      661 gcgtgggcga ggagcggccg ctcggagtgg cgcgcgtgct ggaggcggtg gtgccggcgg
      721 tgcggcggct gcaggagcag ggcaaggtgc gcttcttcgg cgtcaccgcc tccggagaga
      781 ccggcgcgct ccaccgcgcg ctcagcagcg gggcgatcga caccgcgcag gtggtcttca
      841 acctgctcaa cccgacgggg gcgtacgagg tgcccgcggg cttccccgcg caggactacg
      901 accggctgct gctcctggct cgggagcacg gcgtgggcac catcggcatt cgggcggtgg
      961 cgggcggggc gctgagcggc caggtggcgc gccacccgac cgcgatccca tcggtgagcc
     1021 cgatcgcctc gggccccgac tacgccaccg acgtggctcg cgcccgcgcg ctcgagcccc
     1081 tcgtggccca gggccacgcg ggcagcctca tcgaggccgc gctgcgcttc acgatcaccg
     1141 gcgacgccat gtcgaccgtg cagatcggct gctccgacct cgcccagctc gacttcgccg
     1201 ccgccgcggt caacaagggc cccctctccc ccgccgccct ctccaccctg accagcatct
     1261 ggagccagct cgcgacgcgc tgatcggtaa ggggtcaggt aaggggtcag gtcttacatt
     1321 ccgacatttt cctgacccct ggctagcggt tgggtccgcg gcccatcggg tagggctgcc
     1381 agcgaatgag ggaggtcttg ggaaggacgc ccgggttgac cgcggagcgc ggccacttgc
     1441 cctgcagcac ggtggcgagc tcgcgaccca ggcgccgacg agtctcgggc gccatccgcg
     1501 cggtcgcgga ggcgatgtgc gggctcacca gcacgttgtc catgtgcagc agagcgttgt
     1561 tcgggtccac cggctccacc tcgaacacgt cgagtgcggc gcccgcgatc cagccctgac
     1621 gcagcgcctc ggccagggcg gcctcgttca cggtcggccc ccgcccgttg ttgatgaaga
     1681 tcgcgctctt cttcatggcg cggaactcgc gctcgcccat catgtggcgc gtctccgagt
     1741 tgagcggcgc gtgcatggac aggaagtcgg agcgctggca cagctccacc aggctcgtca
     1801 cgggctccac ccccaccgcg gtcatctcga gctcggcgag gaacggatcg taggcgatca
     1861 cccgcagacc gaaggcgtgg cagcggcgcg ccaccgcctt ggccacgttg ccgaacgaga
     1921 tcaggccgat cgtctggcca tagagccgcg ggatgttcgt gaagtacggc cgtccctcgg
     1981 tccacttgtt gtcgaccgtc aactggtgca tcaagcggag ccggcggtgc gcggcgagga
     2041 acatggccat ggtgtgatcg gccacctcgt cgatgaagac gtcgggcacg ttggtgacca
     2101 cgatgcccgc ctcggtggcc gcgtccacgt ccaccgtgtc cgcccccacg ctgccgagcc
     2161 cgatgacttt gcagttcttg agccccttga tgatggccgc ggtgatgcgc cggttgcgcg
     2221 cgatgaccgc gtcggcatcc tgggccgccg cgatgaactc ttcctcggtc ttggccggca
     2281 cctcgacgat ctcggcgccg atgggatcga gggcctcgcg ctcgatcgag taggccgaat
     2341 cggccaggtc ataggtgatg ccgcccgacg gcttctgcac gacgatcttg aacttcctgg
     2401 ctccggctgc catgccgacg acctcctggg tggtctgagg gtggtgggta tcgctgcggc
     2461 ccccaagcat acgccgcccc cgggcctgcc gtcgaggccc tcttgtgcta gggtggcgct
     2521 gccatgctcg tcggcgcctt cgcggtcgcg cataccccgc agctcctgat ccgcccggag
     2581 accgaggacc gcgatctggt gctgcgcgtg cacgcggcga tggggcgggt caagcggcgg
     2641 ctccaggagc tccggccgga cgcgatcgcg atcgtcgcgg gcgaccacgt ggaggcgttc
     2701 ttcctggacg cggtgcccgc gctcgcggtc tacgtggggc gcgagtgcgc gggcaccttc
     2761 ggtccctacc gctaccgcct cgacatccac gagccgctcg cccgcgcggt ggtcgagcag
     2821 ggcatcgagc gcggcttcga tctgctctac acccaggacg ccaagctcga ctacgccttc
     2881 tacgtgccgc tgcacttcgt gatgccggag cccgcgcggc ccatcgtgcc gctgcacgtc
     2941 aacgtgtacc tgccgcccca gcccacgccg gcgcgctgcc acgcctgggg cgccgcgctc
     3001 ggcgagatcc tccgcgcgcg cccggagcgc gtggccctca tcgcctcggg cggcatgtcg
     3061 cacttcccgg gcaccgaccg ctacgcgtcg cccgagttcg actacgaccg cgcgctgctc
     3121 gacctgctcc gcgacgggcg cggccgcgac ctcgcgcgcg tcaccgggga ggagctcgac
     3181 aagcggggca acatcgagct gcgcacgtgg atcaccctgc tcggcgcggt gggcgacgtg
     3241 cgcgcggagg tgtactgcta cgagccgtcc tggcaccacg gcaacgcggt cgtggagtgg
     3301 ccattggaga ccgcgtgagc gagggcgccc actaccgctt cccgccgtcc gaggcctacc
     3361 cgctcaaccg ctgcctctac gcgctcaaat ccgacccggc cttcatcgag cgcttcctcg
     3421 ccgatccggt cgccgcgacc ctcgagatgg gcctgaccga gggcgagcgg gccgacctgc
     3481 gccgcatgga tcgcgacgcg ctggtggccc gcggcgccca tccctacctg gtcttcatgg
     3541 ccggcctgcg gctcaagatc gccgccgagc ccggcgcgct cgagtacttc tagctcgtcg
     3601 acacctcgag ccgggtggcg accagcttcg cgccccgctc ctcgtagaac gccttcacct
     3661 tcgcgccctc ctggagatcg gcgaacctcg cgtcgcggcc gtcgacctgg atgagggtat
     3721 cgggaacgac ctcgagcgtc ttgccgagca ggccgagaaa gatcgagacg tccacgctct
     3781 cgctccgccc gtccacccgt gtgagcgtgc cctcgatcat gccgggcgcc cgcacgagtc
     3841 ggtccaggcg gggccccgac ggctgcagcg ggtggggccg gccctgggcg aagcctcgcg
     3901 gggcgagggc ggactccgcc accatcgcgg tcgagatcat cagcgtcgcc gccccgagca
     3961 tcgtcgtgaa acgtgtcatc gcatgtcctc ctctcgtgag cgagcccggc ggcagcacgg
     4021 gagccgccgg gctcccccga tacgcaaacc cacgcgggcg tcagggcgcc tgcgtctcga
     4081 tcacccggac ctgcgtcacg atcttgtcgc gcccgccgcc gtcggcgtag cgcgccatga
     4141 tcgagtctcc cacctgggcc ggctgcccag gcccc
//
LOCUS       DASUFA010000225         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1546084, whole genome shotgun sequence.
ACCESSION   DASUFA010000225 DASUFA010000000
VERSION     DASUFA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546084"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFX87_08290"
     CDS             <1..481
                     /locus_tag="VFX87_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX4994951.1"
                     /translation="GTLRGLLAALPFASLRHRPELTMLGRTYASGFEPDLEDWLLARP
                     RRTVLNPAWPWGIWYPLRRGGAFARLTPQEQGAILREHGTMGRAYGDADLAHDVRLAC
                     HGLDTHDNDFVIGLVGRELHPLSHLVQAMRRTAQTSQYLQSLGPFFVGHALWQSPLR"
     gene            478..873
                     /locus_tag="VFX87_08295"
     CDS             478..873
                     /locus_tag="VFX87_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994952.1"
                     /translation="MSRRAVAGRAGQALCAAWLMVAALAPAGCAPLPPARQVTDINLI
                     AGRWQGQIAFSRGSYQLFYLTINPDRTLIASWDGVTRWGKVTLDGGRARFSFYIWSGN
                     LDYLEGSGDRVILLKEDFSHWDAIVRPLT"
     gene            complement(866..1363)
                     /locus_tag="VFX87_08300"
     CDS             complement(866..1363)
                     /locus_tag="VFX87_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4994953.1"
                     /translation="MSLTRSEREHLIQQYADGPARLEGALARVPPEARTWRPAPGEWS
                     AHEVACHCADSETNAAARIRYVVCEAEPVVLGYDENVWAVALDYHGHPVDLALATVRA
                     VRANTAAVLRRLPEEAWAREGRHTASGRYTALDWLAIYAEHLEIHARQIEANLAAWEA
                     AGRPR"
     gene            1366..1893
                     /locus_tag="VFX87_08305"
     CDS             1366..1893
                     /locus_tag="VFX87_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994954.1"
                     /translation="MIVSLRMRVMGLTLAAAAVAWVLLMAAAHAQPERGTARLDHAGV
                     QALLVTLGYQPREVRNESGAEYEIVLRPPGGLAVTTRVTLSKDGTLVWLVAWLKKVPP
                     GRTISGNAILDMLTENDAIGPTHFSYNESRRWFFLNKPVPNQGLTAERLQAELQQLGL
                     TIARTEALWDFDRWK"
     gene            complement(1898..>2992)
                     /locus_tag="VFX87_08310"
     CDS             complement(1898..>2992)
                     /locus_tag="VFX87_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX4994955.1"
                     /translation="VELAIIDRAFAEGWIAPEPPAGRTGKTVAVIGSGPAGLAAAQQL
                     ARAGHQVTVFERADRIGGLLRYGIPEFKMEKRVLDRRLAQMEAEGVRFVVGAHVGVDR
                     PVEALRREFDALVLCGGACAPRDLPIPGRELGGVHFAMDYLTPQNRRCEGDVVPDEAF
                     ISAQGRRVVIIGGGDTGADCLGTAHRQGALEVHQLEILPRPPDTRAADNPWPQWPNIY
                     RVSSAHAEGGERVYSVSTTRFIGDERGRVKALEVARVEPVREGGRLVFREVPDSTFVL
                     PCELVLLAMGFVGPERPGLLEQLGVALTDRGNVKRDENWMTTVPGVFTAGDMQRGQSL
                     IVWAIAEGRSAARGVDRYLMGRSDLPAPLP"
BASE COUNT          394 a         1138 c         1051 g          409 t
ORIGIN      
        1 gggcacgctc cgcgggctcc tcgccgccct ccccttcgcc tcgctccggc accgccccga
       61 gctgaccatg ctcggtcgca cctacgcttc gggcttcgag ccggacctcg aggactggct
      121 gctcgcgcgc ccgcggcgga ccgtgctgaa tccggcctgg ccctggggca tctggtaccc
      181 gctccgccgg ggcggtgcct tcgcgcggct gactccgcag gagcaggggg cgatcctgcg
      241 cgagcacggc accatgggcc gggcctatgg cgacgcggac ctggcccatg acgtgcggct
      301 tgcctgccac ggtctcgaca cgcacgacaa cgacttcgtg atcgggctcg tcgggcgcga
      361 gctccacccg ctgtcccacc tggtgcaggc catgcgccgg accgcgcaga cctcgcagta
      421 cctccagagc ctcgggccgt tcttcgtggg ccacgcgctc tggcagagcc cgctgcggtg
      481 agtcggcgag ccgtggccgg gcgcgcggga caggccctgt gcgcggcctg gctcatggtg
      541 gccgcgctcg cccccgcggg ctgcgcgccg ctgccgcccg cgcggcaggt caccgacatc
      601 aacctcatcg cgggccgctg gcaagggcag atcgcgttca gccgcgggtc ctaccagctc
      661 ttctacctga cgatcaaccc cgaccgcacc ctgatcgcct cctgggacgg cgtgacccgc
      721 tggggcaagg tcacgctcga cggtgggcgc gcgcgcttca gcttctacat ctggagcggc
      781 aacctcgatt acctcgaggg cagcggcgac cgcgtcatcc tcctcaagga ggacttctcc
      841 cactgggacg cgatcgtccg gcccctcacc tagggcggcc ggcggcctcc cacgcggcca
      901 ggttggcctc gatctgcctg gcgtggatct ccagatgctc ggcatagatc gccagccagt
      961 ccagcgcggt gtagcgcccc gacgcggtgt ggcgcccttc gcgcgcccag gcctcctcgg
     1021 gcaggcgccg gagcaccgcc gcggtattgg cgcgtacggc ccgcacggtg gcgagcgcga
     1081 ggtccaccgg atgtccgtgg tagtcgagcg cgaccgccca cacgttctcg tcgtagccca
     1141 ggaccaccgg ctcggcctcg caaaccacgt agcggatgcg ggcggccgcg ttggtctcag
     1201 agtccgcgca gtggcaggcc acctcgtgcg cggaccactc cccgggcgcc ggccgccacg
     1261 tccgcgcctc gggcgggacg cgggccaggg cgccctcgag gcgcgcgggg ccgtcggcgt
     1321 actgctggat caggtgctct cgctcggatc gcgtgaggct catcgatgat cgtctccctc
     1381 cggatgcggg tgatgggtct gacgcttgcc gcggcggcgg tggcctgggt cctcctcatg
     1441 gccgccgccc acgcccagcc cgagcgcggc accgcgcgcc tcgaccacgc gggcgtgcag
     1501 gccctgcttg tcacgctcgg ctatcagccg cgcgaggtgc gcaacgagtc gggggcggag
     1561 tacgagatcg tcctgcgccc gccgggcggc ctcgcggtca ccacccgcgt gaccctctcc
     1621 aaggacggca cgctggtctg gctcgtggcc tggctcaaga aggtgcctcc cggccggacc
     1681 atcagcggca acgcgatcct cgacatgctg accgagaacg acgccatcgg ccccacgcac
     1741 ttctcctaca acgagagccg ccgctggttc ttcctcaaca agcccgtgcc gaatcagggg
     1801 ctgaccgcgg agcgcctgca ggccgagctg cagcaactcg gcctgacgat cgcgcggacc
     1861 gaagcgctct gggacttcga ccgctggaag tgacgcctca gggcaggggc gcgggcaggt
     1921 ccgagcggcc catcagatag cggtccacgc cgcgcgcggc gctgcggccc tccgcgatgg
     1981 cccacacgat cagcgactgg ccccgctgca tgtcgcccgc ggtgaacacc ccgggcacgg
     2041 tcgtcatcca gttctcgtcg cgcttgacgt tcccgcggtc ggtgagggcg accccgagct
     2101 gctcgagcag ccccggccgc tcgggcccga cgaagcccat cgccagcagc accagctcgc
     2161 agggcaggac gaacgtcgag tccgggacct cgcggaacac gaggcggccg ccctcgcgca
     2221 ccggctccac gcgcgccacc tcgagggcct tcacacggcc gcgctcgtcg ccgatgaagc
     2281 gcgtggtgga caccgagtag acgcgctcgc cgccctccgc gtgggcggag gagacgcggt
     2341 agatgttcgg ccactgcggc caggggttgt cggcggcgcg cgtgtccgga gggcgcggca
     2401 ggatctcgag ctggtggacc tcgagggcgc cttgacgatg tgcggtgccc aggcagtcgg
     2461 ccccggtgtc gccgccgccg atgatcacca cgcgcctgcc ctgggcgctg atgaaggcct
     2521 cgtcgggcac cacgtcgccc tcgcagcgcc ggttctgcgg cgtgagatag tccatggcga
     2581 agtgcacgcc cccgagctcc cgcccgggaa tcgggaggtc ccggggggcg caggcgccgc
     2641 cgcagagcac gagggcgtcg aactcgcgcc gcagcgcctc caccggccga tccacgccga
     2701 cgtgcgcccc caccacgaag cgcacgccct ccgcctccat ctgggcgagc cgacggtcca
     2761 gcacgcgctt ctccatcttg aactcgggga tgccgtagcg aagcagcccg ccgatccggt
     2821 ccgcgcgctc gaagacggtg acctggtggc cggcgcgcgc gagctgctgg gcggcggcga
     2881 ggccggcggg accggagccg atcaccgcga cggtcttgcc ggtccgcccg gcgggcggct
     2941 cgggcgcgat ccagccctcc gcgaacgcgc ggtcgatgat cgcgagctcg ac
//
LOCUS       DASUFA010000226        20520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_858749, whole genome shotgun sequence.
ACCESSION   DASUFA010000226 DASUFA010000000
VERSION     DASUFA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 20520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20520
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858749"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..604
                     /gene="rnhA"
                     /locus_tag="VFX87_08315"
     CDS             158..604
                     /gene="rnhA"
                     /locus_tag="VFX87_08315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX4994956.1"
                     /translation="MPQSSIVMYTDGACSGNPGPGGWGAILIEDGRELELSGGESPTT
                     NQRMELTAPLEGLRSLAGRRRVAIYSDSAYVINCFRDRWYERWRKNGWINAQKKPVDN
                     RDLWEPLIALAEQHDVTWHKVAGHSGDPLNERADALARSAIARLAR"
     gene            complement(694..1530)
                     /locus_tag="VFX87_08320"
     CDS             complement(694..1530)
                     /locus_tag="VFX87_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4994957.1"
                     /translation="MRLKDRVALVTGAGSGIGRAIALRFAQEGARVIVNDVKQETAQE
                     TVDAIASGSEAARGRALAADVADSGQVKRMFAEVDREFGALDILVNNAGIAEGAPGDR
                     EKIRARSDARIAELMGGGPVSTHWDLTQDMSDEAWHRMIGVHLNGTFFCTREALRLMA
                     RRNRGAIINMSSVAALMGLEASPHYSAAKGGILAFTRAVSREVASRGIRVNAICPGYI
                     DTPMTKPISPILQRLIIARTPLGRWGEPEEIAATAAFLASDDASYFTGQWLSPNGGLF
                     IG"
     gene            1735..2523
                     /locus_tag="VFX87_08325"
     CDS             1735..2523
                     /locus_tag="VFX87_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4994958.1"
                     /translation="MLTDHETRREHDDHEERELPREDMSLRERDAQMKFPALEEPAWL
                     DDEADDEMEEPSDSPFRGMWIGIAAAAVTFVLVFAIPQWLGWYDIGPPPQAKREATPE
                     AVISTVTAKPSGAAVVETGPPAVPAVAAPSATAPPVSLPKVVATKETKPTPPHAAPAV
                     AKETRPASSAVARETKPAPPAVHAPASPSRRTFTVQIAAFKNARQANQLAERVKRDGY
                     PADVRRIESSAVPWVVRVGGYTSREQAEAAREALARKGLRGFIL"
     gene            complement(2513..3871)
                     /locus_tag="VFX87_08330"
     CDS             complement(2513..3871)
                     /locus_tag="VFX87_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994959.1"
                     /translation="MILVEHGTIITVDAGRRILTDGSVLIDGAEIVQVGPARDVRPPR
                     VPDRVIDARRMVVAPGFVDTHVHLSEHLNRGLLLDDIPVDRYLPDWLIPLYSVMTAED
                     EEHAALLAGIEMIRTGTTTFCEAGTLFEPAAVAGAIERLGMRAILGRWTWDLESGPDR
                     MRQSTREALRANEAMLEQVNGRGSLVSAWPLLLGFGTCSEELIRGAKALADRHGVGWG
                     MMHLASHPSRKTRDRLPLATLDAWGVLAPNAKLSHMVYLDEADIALLARRGVKISHCP
                     TAGLKHTKGLAAHARVPEMLAGGVCVSLGGDSGNGSNHFDMLRMMYLVANIYKDARLD
                     VTVMPPETVLEMATIRGAEALLMERRIGAIEAGKRADLVFYDRDQPEWRPLLNPLNNL
                     VYAATGSSVRSVMIDGRLVLDDGRITTVDERAIYERVEVLARDQVRRAGLTIESKWPT
                     TG"
     gene            complement(3868..4716)
                     /locus_tag="VFX87_08335"
     CDS             complement(3868..4716)
                     /locus_tag="VFX87_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4994960.1"
                     /translation="MKLVRYGRAGAEKPGLIDGEGRLRDLSRIVRDITPETLTPAGLK
                     RLRAAKPERLPPVKGKPRLGCPLAGIGKIVCIGLNYTDHAEEVKMALPKEPTIFIKAN
                     SAISGPGDPIARPRGAVKLDYEVELVAVIGRAARGVDEGRALRYVAAYCLMNDVSERE
                     FQMEHGGGTTKGKSCDTFAPIGPWLVTADEIVDPQTLGLWTTVNGERRQQGSTRDMVF
                     PVRQLVAYVSRFMSLQPGDLLSTGTPAGVGHGARPPRYLDDGDVVAMGIDGLGTQQHR
                     VIARRS"
     gene            complement(4725..4940)
                     /locus_tag="VFX87_08340"
     CDS             complement(4725..4940)
                     /locus_tag="VFX87_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HEX4994961.1"
                     /translation="MSRYAVNSLLYRLKKDPDFRARFQADARAALAGLDLTDAERAAF
                     VARDMRKINELGGYLHLVMSVPGLAAH"
     gene            complement(4937..5782)
                     /locus_tag="VFX87_08345"
     CDS             complement(4937..5782)
                     /locus_tag="VFX87_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994962.1"
                     /translation="MGKIVFAGAMSHVLDPEYYQRACGDEGRRKTVECMAEIARMGER
                     FTATRPDALIVVADDHLNAFSFNAVPALCVRIGRSVQRMKQDHAEAFDAVLDHMPEEY
                     PLHEDLANQILEKGLDAGFDLAMSWEAPVDHAFLSPVNTMHGTRPIPPLVPIWVNSFV
                     APQPTPKRCFDFGRHIARVVAAGPWKVGIIATGGLSHFPELSLPRVGETDTVFDRKLI
                     HWMEAGEHEPLRNLGVDELHKTGEHEFLNWMVLLGAVTPARAQVRYFGELARINLAAV
                     EWSLP"
     gene            5935..6438
                     /locus_tag="VFX87_08350"
     CDS             5935..6438
                     /locus_tag="VFX87_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4994963.1"
                     /translation="MAQALDELLRTHVARFTDRRADWDAFADARVEGFKRAQHRFIGS
                     GASGKADASVIPAQHFTLSVMFIPPGQGNAPHTHEVEEVFFILEGKVLVFIEDGAGNR
                     AETVLGPWDCVSCPANVIHGFTNVGLEPAYLQVMLGRAKPELMTYTDSDLQARRDEHL
                     RAAGSRG"
     gene            6460..8784
                     /locus_tag="VFX87_08355"
     CDS             6460..8784
                     /locus_tag="VFX87_08355"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4994964.1"
                     /translation="MLVGASPKRKEDGRFVAGHGRYLDDVRVPGLLHVALVRSPHAHA
                     RVMSVDARAARARAGVVAVWTLDDLPELASAHVPPLVPEPKSRAYVHPIMAGRRARHV
                     GEIVAVVVADDPYVAADGAERVAVAYEPLPAALSPEAAAAPGAPRVNETWPDNLVSVT
                     TSAKGSPESAMAGAEVVVSARLAYPRVAGMPLETRGVLAAPDPIGGGLTVWTSTQVPF
                     AVRSGIAPVVGLQEERIRVIVPDVGGGFGVKGHVYPEEILVPVVARRLGRPVKWAETR
                     SEHCLTAAGDRDQVHEARIGLARDGRIVAIDTAFTRDHGASPMLGEAITLNTINHLPG
                     PYRVPSYRGVGRNILTHKTFAAAYRGAGRPEAAFVLDRLLDRAARRIGMDPAELRRRN
                     LIRADEMPSPTGLTYRDGAPITYDPADYPAAFDGLLAALDYKGWRAEQAKRRGSDRPI
                     GLGLCAYVEGTGIGPFEGADIRVDPDGTVFVHLGVCAQGQGHETTLAQIAADELAVPL
                     ESVVVVGGDTNLAGYGMGTIASRVAAVSGPAVQRTAAQVAHKARLVGAELFECAPEDV
                     VLAEGRVHVKGVPDKSVTLGQVARAAVRSRAVAGAGGPGLSACAFFYPDTVTWAFGVQ
                     GVVAEVDLDTCGIRLLKLAAMHDCGRPINPVIVEGQLHGGIAQGLGSALGESLIYDDA
                     GQLLTGTLMDYPLPRADEMPPLEVIHMDFPSAINALGIKGVGESGVISPAAAVANAVE
                     DALADYGIEIDRPPVTAARVFELLRATGRWPARR"
     gene            8839..9705
                     /locus_tag="VFX87_08360"
     CDS             8839..9705
                     /locus_tag="VFX87_08360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4994965.1"
                     /translation="MIERRPFGRTGHQSTATIFGAAALARVTQDEADRALEVLLTHGV
                     NHIDTAARYGDSELRIGPWMARHRKNFFLATKTGSRGARAAREDLHRSLERLRVDSVD
                     LIQIHSLGQPDDWDQVMGPDGALEALVEARAQGLARHIGVTGHGWSIAAMHRRSLARF
                     DFDAILLPYNFFMAQNDRYREAFGEVTAICRERNVAVQTIKAIARGPWAATDRSHATW
                     YQPLERQGDIDRAVHWVLGRPDVFLNTAGDLTLLPRVLDAAARFTRRPPDEEMAAMHD
                     ATRMSSLFGLPT"
     gene            9702..10331
                     /locus_tag="VFX87_08365"
     CDS             9702..10331
                     /locus_tag="VFX87_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994966.1"
                     /translation="MSVVRAGLFAALALAILGAALPAPAQPARDLSRIGVLASRSPEA
                     PGPSEGLRELGYVDGQNVTIVWQWARSDEKKFPALAADLVRLNVDVIVAANNAAVEAA
                     HRATRTIPIVMVLAEDPVASGFVASLARPGGNVTGFSSQWAELFGKRLQLLKEVAPHI
                     SRVAVLWDPTFGTGDWLIRETKGGAPEALGVRLQLVEVQRPASSTTASR"
     gene            10455..10679
                     /locus_tag="VFX87_08370"
     CDS             10455..10679
                     /locus_tag="VFX87_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX4994967.1"
                     /translation="MRKPGVSYPNGVDFVDLYRRAAGFVDKILKGAKPADLPVEQPTK
                     FELVINLKTAKALGLTIPPSVLLRANQVIE"
     gene            10716..11918
                     /locus_tag="VFX87_08375"
     CDS             10716..11918
                     /locus_tag="VFX87_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4994968.1"
                     /translation="MESWERNVWALALVVFIAFVGFQFFSPFLPLYVMELGVTEPTRV
                     ALWSGVLTAVTPAVSGLLGPLWGRFADRVGRKMMMIRSLAGFVIIVAAMGLVTSVTQL
                     LILRVLQGVIAGFSVFAMALASVSCPKDKVPVAIGRVQGAQLLSVAIGPAAGGYVASH
                     FGLRYAFFVTAGMCAISLVGLIFLFDEGRPQAAGGDTAGTRESSFTLRDVLGLRGFPL
                     VLGLLFISQFIDRGLSLLVPLKVAALPDVTRIAATSGEIISLAAICATGSALAAGRLA
                     QRWPPERLLLIGLVLGGLPCALMALAPGWQSLMLLRCLTGFCLGGALTLAYSLGGTLV
                     PGERRGAAFGWLALSVQVGTAASPLVSGGLAALSLSGAFVLDGTLAWLGAAVLLIGTR
                     RLRRGAAS"
     gene            complement(11922..12767)
                     /locus_tag="VFX87_08380"
     CDS             complement(11922..12767)
                     /locus_tag="VFX87_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994969.1"
                     /translation="MGETRLALRRFVRRRAALVGAVYLVLLIAGALLAPVVAPYDPIE
                     QDLGRMLTAPSSAHLLGTDDLGRDVLTRLLYGARLSLGAACLAVAIATLAGVPIGLVA
                     GYLGGALDDALMRVIDALQAFPALILAMAIAAALGPGLFNVMVAVGIVYTPRFARLVR
                     GQVLSLREEPFVESARAAGASHARVLARHVLPNVVAPVVVQVSIGVAFALLAETALSF
                     LGVGIKPPEPSWGTDVGRGYRFMRLAPWLVFMPGTTILLTALAFNLVGDGIRDAFDPR
                     QSREA"
     gene            complement(12760..13707)
                     /locus_tag="VFX87_08385"
     CDS             complement(12760..13707)
                     /locus_tag="VFX87_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994970.1"
                     /translation="MGRFLRRRLLAVVPLLLLVTSMVFSLVLLLPGDPAVALVGMEDA
                     SEEKLAAIRERLGLNRPLIVQYGSWLGRMVRGDLGVSLRTGQPVTEAIWDRAPVTIGL
                     ALAATAFGLLVGVPLAVVAAHRRGSLVDAAASGLAAFGVAVPNFWLGSMFVLVLALHL
                     RWLPATGYVSPFESGSDAFRHLLLPTLTLGASAVAEVTRQLRSSLGEVLAADYVKTAR
                     AKGLAEARVVAKHAMRPALIPVVTVAGLTVSRLVSNTVVVESIFALPGLGRLNLEAVL
                     TRDFPMLQGAVLVTVLAVIAVNVLADLLYGFIDPRIRYG"
     gene            complement(13720..15249)
                     /locus_tag="VFX87_08390"
     CDS             complement(13720..15249)
                     /locus_tag="VFX87_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4994971.1"
                     /translation="MARSLEATAAAWLAALLLLVAGSSAEAQAPRSGGTLRAALRAEV
                     STFDVHKGASGSDHMYLYPVFDTLVRFDDKMSPRPGLAELWETPDPKTLVLKLRKGVK
                     FHDGTPFNAEAVRFNLARAQDRQTSSMASELTSIEQVEVVDPYTVRLKLKAPDASLIL
                     AFADRAGMMISPAAVQKLGDQFGRQPVGAGEFRMVRWQPGSEVRMERFADYYEPGRPY
                     LDAIVFRIIPDADTRVSALRSGQVDFIMEVPAQDFASLKGERNLRAIEGPTLAYWRVF
                     LNMAKAPLEKKAVREAMNLALDRPTLLRTIIFGLGEVAASPFPAGYWAHNPSLKPWPH
                     DPARAKAKLAEAGLPNGFTMDMVLEPTPEHVRRAEAIQAQWGAVGIKVDLKPMDLAKG
                     VQSFFRGKELMAANYRWTGRPDPDQSVRGMFHSTGFYNPGGHKVPRIEELMDQAKATY
                     RQEDRRRIYQQLSEVIQQEAMDTGIYVAYSLEAMNTMLQGYQPNFLGKPMFRGVWLQG
                     K"
     gene            15429..16358
                     /locus_tag="VFX87_08395"
     CDS             15429..16358
                     /locus_tag="VFX87_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994972.1"
                     /translation="MPLDPQAQTVVDTIAALNLKPISQSTPDEAREAMRTRTAGLGPV
                     EDVPAVADHRVPVTGGEITVRVYSPAGHGPHPVLVFYHGGGWVIGDLYTHDGICRSLV
                     NAAGCAVASVDYRLAPEFKYPTPVDDSWAGLQWVAANGARLGLDPARMAVGGDSAGGN
                     LAAVMALLARDRRGPRLALQLLIYPVTNYDFGTPSYRENATGYLLTTEDMRWFWRHYL
                     AREEQGREPNASPMLAKSLADLPPALVITAGCDPLRDEGEAYAKRLREAGVSVTTTQY
                     PGMFHGFVRMTRILDQSRALLDEMAGALRKALG"
     gene            complement(16472..17554)
                     /locus_tag="VFX87_08400"
     CDS             complement(16472..17554)
                     /locus_tag="VFX87_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4994973.1"
                     /translation="MRVPARQIQEQLESVFTAWGMSAAHAATTAQMMVETDLRGVDSH
                     GISMLPTYDKEFRAGRLNMRPVWKTVRDMPSMARIDADRSLGHPVSAHAMNMAVDKCL
                     GTGVAVVAVFNSHHFGAAGCYAKIAADRGVIGMVTSATRGVTLVPTFAAEPIMGTNPL
                     AFAAPAKKNASFELDMATTTVAAGKVKVYKLNHKPVPSGWVVDGDGRPVTDEATAFQH
                     VFERPDGGITPLGGSRELGGHKGYGLAVLVHILGGTLAGASFSPLRNKTQRDSDPHNI
                     GHFFMAIDPGAFRDAGDFERDLDDVIDVLHGARRVDPKQPVMVAGDPERFTRAERLEQ
                     GVPVPDDLMTQLRAVVAAANVPFLLA"
     gene            complement(17557..18576)
                     /locus_tag="VFX87_08405"
     CDS             complement(17557..18576)
                     /locus_tag="VFX87_08405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4994974.1"
                     /translation="MKLGIYLNAQHPAADDPARRFAETLEQARLIHDLGFDSIWGGEH
                     HATPGFHYFPLLPMLQRLAAEVPGLSIGTNLVLLPLHNPVEIAEIGAFLDVITGGRFM
                     LGVGLGYRPEEFAIFGVPMAERVSRLTEGVEIIRRLWSEDKVTHRGRHWQLENVTIRP
                     RPVQRPRPPIFVGSQVPAGIARAARIADGWLVVPVPRVDEFAAQVKAFSAARAKEGLP
                     ADAQICRLFEVVCASDEDTAIRRAAPFLLEKYAAYLSWGLEGVSLDPAARPEEQLRRL
                     ATNRFAIGSPAQVIEMLLAQHAAGATHLTMRVSWPGMPQADVLAGIELLGREVLPEVR
                     RRTRS"
     gene            complement(18683..19918)
                     /locus_tag="VFX87_08410"
     CDS             complement(18683..19918)
                     /locus_tag="VFX87_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4994975.1"
                     /translation="MAQEAHDPEAGTSISRRVMLRSVGAAVGAAVLAPRLAGAQTPPS
                     GPVAPPSTITSPPRDFSPRGAPTTYFTDPDVLTVEPMFNGLRQPNAPIQRLWTGALWS
                     EGPAWSSQGRYLVWSDIPNNRQLRWIEDNGHVSVFRTPSNNSNGNTFDFQGRQLSCEH
                     VTRRVVRYELDGSTSIIASMFNGKRLNSPNDVVPHPDGSYWFTDPPYGAQLYEGTVDA
                     PGGPANMAGRLNPKLGQPPELGSYKRELPTAVYRVDKSGALTQVAGEDLVPDPNGLCF
                     SPDFKKLYVVSTGRGPGDTHAGGKGEMYVFDVGADNKLSNGKLFSNFMVDGVKCGPDG
                     VRSDVDGNLWVSSNAGRNVGYSGVTVWTPQGQLIGRIRLPEICGNVCFGGPKRNRLFM
                     AASQSLYAVYTATQGAAPG"
     gene            20060..>20520
                     /locus_tag="VFX87_08415"
     CDS             20060..>20520
                     /locus_tag="VFX87_08415"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEX4994976.1"
                     /translation="MSKRKPKPKRSRTKAMKPKATKKVVKKVAKKKGGAAPARRARRG
                     GRKRTTATRRGREGAEEQGRTVGLLREVLEHHETGPAASAGDLDADWQRAQSSGEEAA
                     GGSVSTPDQDRVDDIGHALGVEQPSTAPLRSSEEMLEERDRRYWELERRAQ"
BASE COUNT         2959 a         7388 c         7256 g         2917 t
ORIGIN      
        1 catgacgatc acaccccagc gtctgcagga gctccgcgcg caggcctcgc tgatcccctg
       61 cgagtcctgc ggccgctacc tctactggcc ttcctgagat tcctcccttg atcccctctc
      121 cccgaagggg agagggccac ggcgaggggc gggtctgatg ccgcaatcct cgatcgtcat
      181 gtacacggac ggtgcctgct ccggcaatcc cgggcccggc ggctggggcg ccatcctcat
      241 cgaggacggc cgggagctcg agctctcggg cggcgagagc cccaccacca accagcgcat
      301 ggagctgacc gccccgctcg aaggcctccg ctcgctcgcg ggccgccggc gcgtcgccat
      361 ctacagcgac agcgcgtacg tgatcaactg cttccgcgac aggtggtacg agcgctggcg
      421 caagaacggc tggatcaacg cccagaagaa gccggtggac aatcgcgacc tctgggagcc
      481 gctgatcgcc ctggccgagc agcacgacgt cacctggcac aaggtcgccg gccacagcgg
      541 cgaccccctc aacgagcgcg ccgacgccct cgcccggagc gccatcgccc gcctcgcgcg
      601 ctagcccggg gtcaggtctt acattccgac atttccgcgc gcggcgtgtc tccgaccgcg
      661 atgaaaatgt cggaatgtaa gacctgaccc cattcagccg atgaagaggc cgccgttggg
      721 ggagagccac tgccccgtga agtaggaggc gtcgtcggag gccaggaagg cggcggtggc
      781 cgcgatctcc tcgggctcgc cccagcggcc gagcggcgtg cgggcgatga tcagccgctg
      841 caagatcggc gatatgggct tggtcatcgg cgtgtcgatg tagcccgggc agatcgcatt
      901 cacccggatc ccgcgtgagg ccacctcgcg cgagaccgcg cgggtgaagg ccaggatgcc
      961 gcccttggcc gcgctgtagt gcggggaggc ctcgaggccc atgagcgcgg ccacgctcga
     1021 catgttgatg atcgcgccgc gattgcggcg ggccatcagg cgcagggcct cgcgcgtgca
     1081 gaagaacgtg ccgttgagat gcaccccgat catccggtgc caggcctcgt cggacatgtc
     1141 ctgggtgagg tcccagtgag tgctcaccgg cccgcccccc atgagctcgg cgatgcgcgc
     1201 gtccgaccgc gcgcggatct tctcgcgatc gccgggcgcc ccttccgcga tccccgcgtt
     1261 gttgaccagg atgtcgagcg cgccgaactc gcggtccacc tccgcaaaca tccgcttcac
     1321 ctgccccgag tcggccacgt cggcggcgag cgcgcgccca cgcgccgcct cactccccga
     1381 cgcgatcgcg tccaccgtct cctgggccgt ctcctgcttg acgtcgttga cgatcacccg
     1441 cgccccttcc tgggcgaagc gcagcgcgat ggcccggccg atgccggagc cggcgcccgt
     1501 caccagggcc acccgatcct tgaggcgcac gttgtctcct ccttcgtggg ttcaggtcgt
     1561 cgggaacggc tgaacgctac gtcgcccgat gcgccgagtc aaggcgcttg ccacccctca
     1621 ccctgccctc tccccggacg ggagagggag acattttttc actgccgcgc agcgtcacca
     1681 caggcagctc ttattcttgc cggaaggcgg cgggggccat gctaaaccga gactatgctg
     1741 accgatcacg agacgcgacg ggagcacgac gatcacgagg agcgcgagct gccgcgcgag
     1801 gacatgagct tgcgcgagcg cgacgcgcag atgaaattcc ccgccctcga ggagcccgcg
     1861 tggctcgacg acgaagccga cgacgagatg gaagaaccgt cggactcgcc gttccggggc
     1921 atgtggatcg ggatcgcagc cgccgcggtc accttcgtgc tggtcttcgc gatcccgcag
     1981 tggctcggct ggtacgacat cggcccgccg ccccaggcca agcgggaggc gacgccggaa
     2041 gcggtcatct ccaccgtgac cgcgaagcct tcgggcgccg ccgtcgtcga gacggggccg
     2101 ccggcggtac cggcggtcgc ggcgccgtcc gccacggcgc cgccggtcag cctcccgaag
     2161 gtcgtggcca cgaaggagac gaagcccacg ccgccccacg ccgcccccgc cgtggcgaag
     2221 gagacgaggc cggcctcgtc cgccgtcgcg agggagacga agccggcgcc gcccgccgtc
     2281 cacgcgcccg cgagcccttc ccgacggacg ttcacggtcc agatcgcggc cttcaagaac
     2341 gccaggcagg ccaaccagct cgccgagcgc gtgaagcgcg acgggtatcc cgccgacgtg
     2401 cggcggatcg agtcgtccgc ggtgccgtgg gtcgtgcggg tcggcggcta cacctcgcgc
     2461 gagcaggccg aggcggcccg cgaggcgctg gcccgcaagg gcctccgcgg cttcatcctg
     2521 tagtcggcca cttgctctcg atggtgagcc ccgcgcgccg gacctggtcg cgcgccagca
     2581 cctccacccg ctcgtagatc gcccgctcgt ccaccgtggt gatgcggccg tcgtcgagca
     2641 cgagccggcc gtcgatcatc acgctccgca cgctcgatcc ggtcgccgcg tagaccaggt
     2701 tgttgagcgg attgagcagc ggccgccact ccggctgatc gcgatcgtag aagaccaggt
     2761 cggcccgctt gccggcctcg atcgcgccga tccggcgctc catcagcagc gcctccgccc
     2821 cgcggatcgt cgccatctcc agcaccgtct cgggcggcat cacggtcacg tcgaggcgcg
     2881 cgtccttgta gatgttggcg acgaggtaca tcatgcgcag catgtcgaag tgattggagc
     2941 cgttgccgct gtcgccgccg agcgagacgc acacgccccc cgccagcatc tcgggcacgc
     3001 gcgcgtgggc cgccagcccc ttcgtgtgct tgagcccggc ggtgggacag tggctgatct
     3061 tcaccccccg ccgcgcgagc aacgcgatgt cggcctcgtc gagatacacc atgtgcgaga
     3121 gcttcgcatt cggcgcgagc acgccccagg cgtccagcgt ggccagcggc aggcggtccc
     3181 gcgtcttccg ggagggatgc gaggcgaggt gcatcatccc ccatccgacc ccgtgccggt
     3241 cggccagcgc cttggccccg cggatcagct cctcggagca ggtgccgaac cccagcagca
     3301 gcggccacgc ggacacgagc gacccacgac cattcacctg ctcgagcatc gcctcgttcg
     3361 cgcgcagggc ttcccgcgtg ctctggcgca tgcggtccgg ccccgactcg aggtcccacg
     3421 tccagcgccc gaggatcgcc cgcatgccga ggcgctcgat cgcccccgcc accgcggccg
     3481 gctcgaagag cgtgcccgcc tcgcagaacg tggtggtacc ggtgcggatc atctcgatgc
     3541 cggccaggag cgccgcgtgc tcctcgtcct ccgcggtcat gaccgagtag agcgggatca
     3601 gccagtcggg caggtagcgg tccaccggga tgtcgtcgag gagcaggccg cggttgagat
     3661 gctcggagag gtgcacgtgg gtgtccacga agccgggggc caccaccatc cggcgcgcgt
     3721 cgatcacccg gtccggcacc cggggcgggc gcacgtcgcg cgcgggcccg acctggacga
     3781 tctcggcccc gtcgatcagg acggatccgt cggtgaggat ccgccgcccg gcatccaccg
     3841 tgatgatggt gccgtgctcg acgaggatca cgagcggcgg gcgatcaccc ggtgctgctg
     3901 ggtcccgaga ccgtcgatcc ccatcgccac cacgtcgccg tcgtcgaggt agcgcggcgg
     3961 ccgggccccg tggcccactc cggcaggcgt gccggtggag agcagatcgc ccggctggag
     4021 cgacatgaac cggctcacgt aggcgacgag ctggcggacc gggaagacca tgtcccgggt
     4081 gctgccctgc tggcgtcgct cgccgttgac ggtggtccag agcccgagcg tctgcggatc
     4141 gacgatctcg tccgcggtga ccagccaggg cccgatgggc gcgaaggtgt cgcagctctt
     4201 gcccttggtg gtcccgcccc cgtgctccat ctggaactcg cgctcggaga cgtcgttcat
     4261 caggcagtag gcggccacgt accggagcgc gcggccctcg tccacgccgc gcgcggcgcg
     4321 gccgatcacc gccaccagct ccacctcgta atcgagcttc accgcgcccc gcggccgcgc
     4381 gatcggatcg cccggcccgc tgatcgcgct gttggccttg atgaagatcg tgggctcctt
     4441 cggcagcgcc atcttcactt cttccgcgtg atcggtgtag ttgaggccga tgcagacgat
     4501 cttgccgatg ccggccaacg ggcagccgag ccggggcttg cccttcaccg ggggtaggcg
     4561 ctcgggcttc gccgcgcgca ggcgcttgag cccggcgggc gtgagcgtct ccggggtgat
     4621 gtcccggacg atccgggaga gatctcgcag tcttccttct ccgtcgatca acccgggctt
     4681 ctcggcgccg gcccgtccgt agcgtacgag tttcatgggg ctcctcagtg ggccgcgagg
     4741 ccgggaaccg acatcacgag gtgcagatag ccgccgagct cgttgatctt ccgcatgtcg
     4801 cgggccacga aggcggcgcg ctcggcgtcc gtgagatcga gcccggccag cgctgcccgc
     4861 gcgtcggcct ggaagcgcgc ccggaagtcg gggtctttct tgagccggta gagcaggctg
     4921 ttgaccgcgt accggctcac ggcaggctcc actcgacggc ggccaggttg atccgcgcca
     4981 gctcgccaaa gtagcgcacc tgggcgcgcg ccggtgtcac cgcccccagc agcaccatcc
     5041 agttcaggaa ctcgtgctcg cccgtcttgt gcagctcgtc cactccgagg ttgcgcaggg
     5101 gctcgtgctc gccggcctcc atccagtgga tcagcttgcg gtcgaagacg gtatcggtct
     5161 cgccgacccg cggcagcgac agctcgggaa agtgcgagag ccccccggtg gcgatgatgc
     5221 ccaccttcca cggccccgcc gccaccaccc gcgcgatatg ccggccgaag tcgaagcagc
     5281 gcttcggcgt gggctggggt gccacgaagg agttcaccca gatgggcacc agcggcggga
     5341 tgggccgcgt tccgtgcatg gtgttcaccg ggctcaggaa ggcgtggtcc accggcgcct
     5401 cccaggacat ggccaggtcg aatccggcgt ccagtccctt ctcaagaatc tggttggcca
     5461 ggtcctcgtg cagcgggtac tcctccggca tgtggtcgag caccgcgtcg aacgcctcgg
     5521 cgtggtcctg cttcatccgc tgcacgctgc ggccgatgcg cacgcagagc gcgggcaccg
     5581 cgttgaacga gaacgcgttc aggtggtcgt cggccaccac gatgagcgcg tcgggccggg
     5641 tcgcggtgaa ccgctcgccc atgcgggcga tctcggccat gcactcgacg gtcttgcgtc
     5701 gaccctcgtc gccgcacgca cgctggtagt attcggggtc gaggacgtga gacatggcgc
     5761 cggcgaagac gatcttgccc atgcgggcct ccttggagag cggtccgccg cggatcgtag
     5821 cggatggcct ccgcggacgc aaggcgcggc gcggagtctg cgatcggccg gcgcgcgcgc
     5881 cttgcggccc cgatgctctg cgactagact ggggcccccg ggagggacac cgacatggcc
     5941 caggcactcg acgagctgct ccgcacccac gtggcccgct tcaccgaccg ccgcgccgac
     6001 tgggacgcct tcgccgacgc gcgcgtggag ggcttcaagc gcgcgcagca tcgcttcatc
     6061 ggctccggcg cctccggcaa ggccgacgcc tcggtcatcc cggcccagca cttcacgctc
     6121 tccgtgatgt tcatcccccc cggccagggc aatgcgccgc acacccacga ggtcgaggag
     6181 gtcttcttca tcctcgaggg caaggtgctg gtcttcatcg aggacggcgc cggcaaccgc
     6241 gccgagaccg tgctgggccc gtgggactgc gtctcctgcc cggccaacgt catccacggc
     6301 ttcaccaacg tggggctcga gcccgcgtat ctccaggtga tgctcggccg agccaagccc
     6361 gagctgatga cctataccga cagcgatctg caggcccgcc gcgacgagca cctccgcgcc
     6421 gcgggctccc gcggctagcg tcccggtccc cgctcccacg tgcttgtcgg cgcctcaccg
     6481 aagcggaagg aagacggccg cttcgtcgcg ggccacggcc gctacctcga cgacgttcgc
     6541 gtgccggggc tgctccacgt ggccctggtg cggagcccgc acgcccacgc gcgcgtgatg
     6601 tcggtggacg cgcgggccgc ccgcgcgcgg gccggcgtgg tcgcggtgtg gacgctcgac
     6661 gatctacccg agctggcctc ggcacacgtg cccccgctcg tgccggagcc gaagagccgg
     6721 gcgtacgtgc acccgatcat ggcgggcagg cgggcgcgcc acgtgggcga gatcgtcgcg
     6781 gtcgtggttg cggacgatcc ctacgtggcg gccgacggcg ccgagcgcgt cgcggtggcc
     6841 tacgagccgc tgcccgccgc gctgtcgccg gaggcggccg ccgcgcccgg cgccccgcgc
     6901 gtcaacgaaa cgtggccgga caacctggtc tcggtgacca ccagcgccaa gggcagtccc
     6961 gagagcgcga tggccggcgc ggaggtggtc gtctccgcgc gcctggccta tccgcgcgtg
     7021 gccgggatgc cgctcgagac gcgcggcgtg ctggccgcgc ccgatccgat cggcggcggc
     7081 ctcacggtgt ggacctccac ccaggtgccc ttcgcggtgc gcagcggcat cgccccggtg
     7141 gtgggcctgc aggaggagcg tatccgcgtg atcgtgcccg acgtgggcgg cggcttcggg
     7201 gtgaaggggc acgtctatcc cgaggagatc ctggtgcccg tggtggcccg gcgcctcggg
     7261 cggccggtga agtgggccga gacgcgcagc gagcactgcc tcaccgccgc cggggaccgc
     7321 gatcaggtcc acgaggcgcg gatcgggctc gcgcgcgacg gccgtatcgt ggccatcgac
     7381 accgcgttca cccgcgacca cggcgcctcg cccatgctcg gcgaggcgat cacgctgaac
     7441 accatcaacc acctgcctgg cccctaccgc gtgccgagct accgcggggt cggccggaac
     7501 atcctcaccc acaagacctt cgccgcggcc taccgcggcg cgggccggcc cgaggccgcc
     7561 ttcgtgctgg accggctgct ggatcgtgcg gcccggcgta tcggcatgga ccccgccgag
     7621 ctgcgccggc gcaacctgat ccgcgccgac gagatgccga gccccaccgg gctcacctat
     7681 cgcgacggcg cgccgatcac ctacgacccg gccgactacc cggcggcctt cgacgggctc
     7741 ctcgccgccc tcgactacaa gggctggcgg gccgagcagg cgaagcggcg cgggagcgac
     7801 cggcccatcg ggctcgggct ctgcgcctac gtcgagggca ccggcatcgg gcccttcgag
     7861 ggcgcggaca tccgcgtgga tccggacggc accgtgttcg tgcacctggg cgtctgcgcc
     7921 cagggccagg ggcacgagac cacgctggcc cagatcgcgg ccgacgagct ggcggtgccg
     7981 ctcgagtcgg tggtggtggt gggcggggac accaacctgg ccggctacgg catgggcacg
     8041 atcgcaagcc gcgtggccgc ggtgagcggc cccgcggtgc agcgcaccgc cgcgcaggtc
     8101 gcccacaagg cgcgcctggt cggcgccgag ctcttcgagt gtgcgcccga ggacgtcgtg
     8161 ctggccgagg gccgcgtgca cgtgaagggc gtccccgaca agagcgtgac gctcggccag
     8221 gtggcccgcg ccgcggtgcg gagccgcgcg gtggccgggg cgggcgggcc ggggctcagt
     8281 gcctgcgcct tcttctatcc cgacacggtc acgtgggcct tcggcgtgca gggcgtcgtc
     8341 gccgaggtgg atctggacac gtgcggcatc cggctgctca agctggcggc catgcacgac
     8401 tgcgggcggc cgatcaaccc ggtgatcgtg gaaggccagc tgcacggcgg catcgcccag
     8461 ggcctcggca gcgcgctcgg cgagtcgctg atctatgacg acgcgggcca gctcctcacc
     8521 ggcacgctca tggactaccc gttgccgcgc gccgacgaga tgccgccgct cgaggtcatc
     8581 cacatggact tcccgtccgc gatcaacgcc ctcggcatca agggcgtggg cgagagcggg
     8641 gtcatctcgc ccgcggccgc ggtggccaac gcggtggagg acgccctcgc cgactacggc
     8701 atcgagatcg accgtccccc ggtcaccgcc gcgcgcgtgt tcgagctgct gcgcgccacc
     8761 gggcgctggc cggctcggcg atgaatcccc tcaccccggc cctctcccct ctggggagag
     8821 ggtgatcgga ggcaaaccat gatcgagcgc cggccgttcg gacgcaccgg gcaccagagc
     8881 actgccacga tcttcggcgc cgccgcgctg gcgcgcgtca cccaggacga ggccgatcga
     8941 gcgctggagg tgctgctcac ccacggggtg aaccacatcg acaccgcggc gcgctacggc
     9001 gactccgagc tgcgcatcgg gccgtggatg gcgcggcacc ggaagaactt cttcctggcc
     9061 accaagaccg gctcgcgcgg ggcgcgggcc gcgcgcgagg atctccaccg ctcgctcgag
     9121 cgcctccgcg tggactccgt ggatctcatc cagatccatt cgctcggcca gcccgacgac
     9181 tgggatcagg tgatgggccc ggacggcgcg ctcgaggcgc tggtggaggc acgggcgcag
     9241 gggctcgcgc gccacatcgg cgtcaccggc cacggctgga gcatcgcggc catgcaccgg
     9301 cggagcctcg cccgcttcga cttcgacgcg atcctgctgc cctacaactt cttcatggcc
     9361 cagaacgacc gctaccgcga ggccttcggc gaggtcaccg ccatctgccg ggagcgcaac
     9421 gtggcggtgc agaccatcaa ggccatcgcg cgggggccgt gggccgcgac cgaccggagc
     9481 cacgccacgt ggtaccagcc gctggagcgg cagggcgaca tcgatcgggc ggtccactgg
     9541 gtgctgggcc gccccgacgt cttcctcaac accgcgggcg acctcaccct gctgccgcgc
     9601 gtgctcgacg cggcggcccg cttcacgcgc cgccccccgg acgaggagat ggcggccatg
     9661 cacgacgcga cgcggatgtc ctcgctcttc ggcctgccca cgtgagcgtg gtgcgcgccg
     9721 ggctcttcgc cgctctcgcg ctcgcaatcc tcggcgccgc gctcccggcc ccggcccagc
     9781 ccgcgcggga cctgtcgcgc atcggcgtgc tcgcgagccg ctcgcccgag gcgccaggtc
     9841 cctccgaagg cctccgcgaa ctgggctacg tggatgggca gaacgtgacg atcgtctggc
     9901 agtgggcgcg gtcggacgag aagaagttcc ccgcgctcgc cgccgacctg gtgcggctca
     9961 acgttgacgt gatcgtggcc gccaacaatg cggcggtcga agccgcgcac cgggcgaccc
    10021 ggacgatccc gatcgtcatg gtgctcgcgg aggatcccgt cgcctccggg ttcgtcgcga
    10081 gcctcgctcg tccgggtgga aacgtcacgg gattctccag tcagtgggcc gagctgttcg
    10141 gcaagcgcct gcagcttctc aaggaggtcg cccctcacat ctcgcgggtg gccgtgctct
    10201 gggatcccac cttcgggacc ggcgactggc tgatccgaga gacgaagggc ggcgctcccg
    10261 aggcgctcgg ggtgcgcctc cagctcgtcg aggtgcaaag gccggcgagc tcgacgacag
    10321 cttcgcggtg ataacgaggg aacgatcggg cgcgctcctg atccagggga gctcgatgct
    10381 catgggtcag cggacgcggt tgagagacct cacggtcaag agccggctgc cctcgatgtg
    10441 cacgctacgg gagtatgcgg aagccgggtg tctcgtatcc taatggtgtg gatttcgtcg
    10501 acctgtaccg gcgagcggcg gggttcgtcg acaagatcct caagggcgcg aagcccgcgg
    10561 atctcccggt ggagcagccc accaagttcg agctcgtgat caatctgaag accgccaagg
    10621 cgctcggcct gacgatcccc ccgtcggtgc tgctgagggc gaaccaggtc atcgagtaat
    10681 ccggtccgtt catgaatagt ccgggctagc ggccggtgga gagctgggag cgcaacgtct
    10741 gggccctagc cctcgtcgtc ttcatcgcgt tcgtcggctt ccagttcttc tcgcccttcc
    10801 tgccgctcta cgtgatggag ctgggcgtca ccgagcccac gcgcgtggcc ctctggtccg
    10861 gcgtgctgac cgcggtgacc ccggcggtct cgggcctgct gggcccgctc tgggggcgct
    10921 tcgccgaccg cgtgggtcgc aagatgatga tgatccgctc gctcgccggc ttcgtgatca
    10981 tcgtggccgc gatgggcctc gtgacctcgg tcacccagct cctcatcctg cgagtgctcc
    11041 agggcgtcat cgcgggcttc tccgtcttcg ccatggccct ggcgagcgtg tcgtgcccga
    11101 aggacaaggt gccggtggcc atcgggcgcg tgcagggcgc gcagctcctg agcgtggcga
    11161 tcgggccggc ggcgggcggg tacgtggcct cccatttcgg cctgcgctac gccttcttcg
    11221 tcaccgcggg gatgtgcgcg atctccctcg tcgggctgat cttcctcttc gacgagggcc
    11281 ggccgcaggc cgccggcggc gacaccgcgg gcacgcgcga gagcagcttc acgctgcgcg
    11341 acgtgctggg cctgcgcggg ttcccgctcg tgctgggcct gctcttcatc agccagttca
    11401 tcgaccgcgg gctgtcgctg ctggtgcccc tgaaggtcgc ggccctgccc gacgtgaccc
    11461 gcatcgcggc gacctcgggc gagatcatct cgctcgccgc catctgcgcc accggctcgg
    11521 ccctcgccgc cggccggctc gctcagcgct ggccgcccga gcggctgctg ctgatcggcc
    11581 tggtgctggg cggcctgccc tgcgcgctca tggccctggc gcccggctgg cagagcctca
    11641 tgctgctgcg ctgcctcacc ggcttctgcc tgggtggcgc gctgaccctg gcctactcgc
    11701 tcggcggcac gctggtgccg ggcgagcgcc gcggcgccgc cttcggctgg ctcgcgctga
    11761 gcgtgcaggt gggcaccgcg gcgagcccgc tcgtcagcgg cggactggcc gcgctgagtc
    11821 tctccggcgc cttcgtgctg gacggtacgc tcgcctggct gggtgccgcg gtgctcctca
    11881 tcggcacgcg ccgcctgcgc cgcggcgccg cctcctgaac gctacgcttc gcggctctgg
    11941 cgcgggtcga acgcgtcgcg gatgccgtcg ccgaccagat tgaacgccag cgcggtcagg
    12001 agaatcgtgg tccccggcat gaagacgagc cacggggcca ggcgcatgaa gcggtagccg
    12061 cggcccacgt cggtgcccca cgagggctcg ggcggcttga tgcccacgcc caggaaggac
    12121 agcgcggtct cggccagcag cgcgaaggcg acgccgatgg acacctgcac caccaccggg
    12181 gccaccacgt tcggcagcac gtggcgcgcc agcacgcgcg cgtggctggc gccggcggcg
    12241 cgcgcgctct ccacgaacgg ctcctcgcgc agcgagagca cctgcccgcg cacgaggcgg
    12301 gcgaagcgcg gcgtgtagac gatgcccacc gccaccatca cgttgaagag cccgggaccg
    12361 agggccgccg cgatggccat ggccaggatc agcgccggga aggcctggag cgcgtcgatc
    12421 acccgcatca gcgcgtcgtc gagggccccg cccaggtagc ccgcgaccag gccgatgggc
    12481 acgccggcca gcgtggcgat ggccaccgcc aggcacgcgg cgcccagcga gaggcgcgcg
    12541 ccgtagagca ggcgcgtgag cacatcgcgg cccaggtcgt cggtgccgag gagatgcgcg
    12601 gacgagggcg cggtcagcat gcggccgaga tcctgctcga tcggatcgta cggggccacc
    12661 accggcgcga gcagcgcgcc cgcgatcagc agcacgaggt agaccgcgcc caccagcgcg
    12721 gcacggcgcc gcacgaagcg ccggagcgcg aggcgggtct cacccatagc ggatgcgcgg
    12781 gtcgatgaag ccgtagagca ggtccgcgag cacgttgacc gcgatcaccg cgagcaccgt
    12841 gaccagcacc gcgccctgga gcatcgggaa gtccctggtg agcaccgcct ccaggttgag
    12901 ccggccgagg ccgggcagcg cgaagatgga ctccaccacg acggtattgc tgaccagccg
    12961 cgagaccgtg agcccggcca ccgtcaccac cgggatgagg gccggccgca tcgcgtgctt
    13021 ggccaccacg cgggcctcgg ccaggccctt ggcgcgcgcg gtcttcacgt agtcggcggc
    13081 cagcacctcg cccagcgagg agcggagctg gcgcgtgacc tccgccaccg cggaggcgcc
    13141 cagggtgagg gtgggcagca gcaggtggcg gaacgcgtcg ctccccgact cgaacgggct
    13201 cacgtagccg gtggcgggca gccagcgcag gtggagagcc aggaccagca cgaacatgga
    13261 gccgagccag aagttcggca ccgcgacccc gaacgcggcg agcccgctcg ccgccgcgtc
    13321 caccaggctg ccgcggcggt gggccgccac caccgcgagc ggcacgccca ccagcaggcc
    13381 gaaggcggtc gcggccaatg ccagcccgat cgtcaccggc gcgcggtccc agatcgcctc
    13441 ggtcaccggc tggccggtgc ggagcgacac gccgagatcc ccgcgcacca tccgcccgag
    13501 ccagctgccg tactgcacga tgagcggccg gttgagtccg aggcgctcgc ggatcgccgc
    13561 cagcttctcc tcgctcgcgt cctccatccc gaccagcgcc accgccggat cgcccggcag
    13621 cagcaggacg agcgagaaca ccatcgaggt gacgagcagc agcagcggca ccaccgcgag
    13681 cagccggcgc cggaggaagc gccccacgtc ggccccgttc tacttgcctt gcagccagac
    13741 cccgcggaac atcggcttgc cgaggaagtt gggctggtag ccctgcagca tcgtgttcat
    13801 ggcctcgagc gagtacgcca cgtagatgcc ggtatccatc gcctcctgct ggatcacctc
    13861 gctgagctgc tggtagatcc gccgccggtc ctcctggcga taggtcgcct tggcctggtc
    13921 catcagctcc tcgatgcgcg ggaccttgtg gccgcccgga ttgtagaacc cggtggagtg
    13981 gaacatgccg cgcacgctct ggtcggggtc gggccgcccg gtccagcggt agttggccgc
    14041 catcagctcc ttgccgcgga agaaggactg cacgcccttg gccaggtcca tcggcttgag
    14101 gtccaccttg atgccgaccg cgccccactg cgcctggatg gcctccgcgc ggcgtacgtg
    14161 ctccggggtg ggctccagca ccatgtccat cgtgaagccg ttgggcaggc ccgcctcggc
    14221 cagcttggcc ttggcgcgcg ccggatcgtg cggccacggc ttgagcgacg ggttgtgcgc
    14281 ccagtagccg gccgggaacg gcgaggcagc cacctcgccc aggccgaaga tgatcgtgcg
    14341 cagcagcgtc gggcgatcga gcgccaggtt catcgcctcg cgcaccgcct tcttctcgag
    14401 cggcgccttg gccatgttga ggaagacgcg ccagtaggcg agcgtcgggc cctcgatggc
    14461 gcgcaggttg cgctcgccct tcagcgacgc gaagtcctgg gcgggcacct ccatgatgaa
    14521 gtccacctgg ccgctgcgga gcgcggagac gcgggtgtcg gcgtccggga tgatccggaa
    14581 cacgatggcg tcgaggtacg ggcgccccgg ctcgtagtag tcggcgaagc gctccatccg
    14641 cacctcgctg ccgggctgcc agcgcaccat ccggaactcg cccgccccca ccggctgacg
    14701 gccgaactgg tcgcccagct tctgcaccgc ggcgggcgag atcatcatgc ccgcgcgatc
    14761 cgcgaaggcc aggatcagcg aggcgtccgg cgccttgagc ttgaggcgca ccgtgtacgg
    14821 gtccaccacc tccacctgct cgatgctggt cagctcggag gccatggacg aggtctgccg
    14881 atcctgggcg cgggccaggt tgaagcgcac cgcctccgcg ttgaacggcg tgccgtcgtg
    14941 gaacttgacg cccttccgca gcttgagcac gagcgtcttc ggatccggcg tctcccacaa
    15001 ctcggccagc cccggccggg ggctcatctt gtcgtcgaag cggaccaggg tgtcgaacac
    15061 cgggtagagg tacatgtggt cggagcccga cgcgcccttg tgcacgtcga aggtcgacac
    15121 ctcggcacgc agggccgcgc gcagggtgcc gccggaccgc ggggcctgcg cctccgcgga
    15181 agagccggcg acgagcagca ggagcgcggc gagccaggcg gccgccgtcg cctcgagaga
    15241 acgggccatg agagctttcc tccttgaacg gtgagcggaa cgcaggagcg ttggagacgc
    15301 ggcgagtgta cgtgccggcg ccgcgggctg tcaatgcgcg ccgccgctcg acgaagcatc
    15361 ccttgacgac gcgggcggga ttctctactg tcgccgcatc acctgaatcg cccccgggag
    15421 gacacgccat gccgctcgat ccccaggccc agacggtcgt ggacaccatc gccgcgctga
    15481 acctcaagcc gatctcgcag tccacccccg acgaggcgcg cgaggccatg cgcacgcgca
    15541 ccgccgggct cggcccggtc gaggacgtgc ccgccgtcgc ggaccaccgc gtgccggtga
    15601 cgggcggcga gatcacggtg cgggtctaca gccccgccgg ccacggaccg catccggtcc
    15661 tggtcttcta ccacggcggc ggctgggtga tcggcgacct ctacacccac gacggcatct
    15721 gccggtccct cgtcaacgcg gcgggctgcg cggtggcctc ggtggactac cggctcgcgc
    15781 cggagttcaa gtacccgacg ccggtggacg actcctgggc ggggctccag tgggtggccg
    15841 ccaacggcgc gcggctcggg ctcgatcccg cccggatggc ggtgggcggc gacagcgcgg
    15901 gcggcaacct cgccgcggtc atggcgctcc tggcgcgcga tcggcgcggg ccgcggctcg
    15961 ccctgcagct cctgatctac ccggtgacga actacgactt cggcaccccg tcctaccgcg
    16021 agaacgccac cggttacctg ctgaccaccg aggacatgcg gtggttctgg cgccactacc
    16081 tggcccgcga ggagcagggg cgcgagccga acgcctcgcc gatgctggcc aagagcctgg
    16141 ccgacctgcc gcctgcgctc gtgatcaccg cgggctgcga tcccctgcgc gacgaggggg
    16201 aagcctacgc caagcggctg cgagaggcgg gcgtctcggt gaccacgaca cagtatcccg
    16261 gcatgttcca cggcttcgtg cggatgacgc gcatcctcga ccagtcgcgc gcgctcctcg
    16321 acgagatggc gggggcgctg cggaaggcgc tgggctaggc gaccgcgcgc gtagaccacc
    16381 cctcaccctg ccctctcccc tctgggagcg gcgggagcga gcagtaccac cctctcccct
    16441 ctggggagag ggcagggtga ggggcctgtg ttcaggcgag caggaacggc acgttcgcgg
    16501 ccgcgaccac ggcgcggagc tgcgtcatca gatcgtcggg gaccggcacg ccctgctcga
    16561 gccgctccgc gcgcgtgaac cgctccgggt cacccgccac catcaccggc tgcttcgggt
    16621 ccacgcgcct ggccccgtgc agcacgtcga tcacgtcgtc caggtcgcgc tcgaagtcgc
    16681 ccgcgtcgcg gaaggcgccc ggatcgatgg ccatgaagaa gtggccgata ttgtgcggat
    16741 ccgagtcgcg ctgcgtcttg ttgcgcagcg gcgagaagga cgcgcccgcg agcgtgccgc
    16801 ccaggatgtg caccagcacc gcgaggccgt agcccttgtg gccgcccagc tcgcggctgc
    16861 cgccgagcgg cgtgatgccg ccgtccgggc gctcgaagac gtgctggaag gccgtcgcct
    16921 cgtcggtgac cggacggccg tcgccgtcca ccacccaccc cgagggcacc ggcttgtggt
    16981 tgagcttgta aaccttcacc ttgcccgccg cgacggtggt cgtggccata tccagctcga
    17041 aggacgcgtt cttcttcgcg ggtgccgcaa aggcgagcgg gttcgtcccc atgatgggct
    17101 ccgccgcgaa ggtgggcacc agcgtgacgc cgcgggtggc ggaggtgacc atgccgatca
    17161 cgccgcggtc ggccgcgatc ttcgcgtagc acccggccgc gccgaagtgg tgcgagttga
    17221 acaccgcgac caccgccacc ccggtgccga ggcacttgtc gaccgccatg ttcatggcgt
    17281 gcgccgacac cggatggccg agcgagcggt ccgcgtcgat ccgggccatc gacggcatgt
    17341 cgcgcaccgt cttccagacc gggcgcatgt tgagccgccc cgcgcggaac tccttgtcgt
    17401 aggtcggcag catcgagatc ccgtgcgagt ccaccccgcg cagatcggtc tccaccatca
    17461 tctgcgcggt cgtggccgcg tgtgcggccg acatgcccca cgcggtgaac acggactcga
    17521 gctgctcctg gatctggcgg gcggggactc gcatggctag ctcctggtgc ggcggcgaac
    17581 ttcggggaga acctcgcggc cgagcagctc gatgccggcc aggacatccg cctgcggcat
    17641 cccgggccag ctcacgcgca tggtgaggtg cgtggcgccg gccgcgtgct gggcgagcag
    17701 catctcgatc acctgggcgg gggagccaat ggcaaagcgg ttggtagcca ggcggcggag
    17761 ctgctcctcc ggcctggcgg cggggtcgag cgagacaccc tcgagtcccc acgagagata
    17821 ggccgcgtac ttctccagca ggaagggcgc ggcgcggcgg atcgcggtgt cctcgtcgga
    17881 ggcgcagacc acctcgaaca gccggcagat ctgcgcatcc gcggggagac cttccttggc
    17941 gcgggccgcg ctgaaagcct tcacctgcgc cgcgaactcg tccacgcgcg gcaccggaac
    18001 caccagccag ccgtccgcga tgcgggcggc ccgcgcgatg cccgcgggga cctgcgagcc
    18061 gacaaagatg ggcgggcgcg ggcgctggac cggccgcggg cggatcgtga cgttctcgag
    18121 ctgccagtgc cgcccgcgat gcgtgacctt gtcctcgctc cagagccgcc ggatgatctc
    18181 cacgccctcg gtgaggcggc tcacccgctc cgccatcggc accccgaaga tcgcgaactc
    18241 ctccgggcgg tagccgagcc caacgcccag catgaagcgc ccgccggtga tcacgtcgag
    18301 aaacgcgccg atctccgcga tctccaccgg attgtgcagc ggcagcagca ccaggttggt
    18361 cccgatcgac agcccgggga cctccgcggc cagccgttgc agcatcggca gcaacggaaa
    18421 atagtgaaac cccggcgtgg catggtgctc cccgccccag atcgagtcga acccgaggtc
    18481 gtggatcagc cgcgcctgct ccagcgtctc ggcaaaccgc cgcgccggat catcagcagc
    18541 cggatgctgc gcgttgaggt agatccccag cttcatggcg aagatagatg gggtcaggtc
    18601 ttacattcca acattttccg cgcgctccgt gttcgacgaa cgcgcggaaa atgttggaat
    18661 gtaagacctg accccggccg ggttaaccgg gcgcggcgcc ctgcgtggcg gtgtagaccg
    18721 cgtagaggga ctggctggcc gccatgaaga ggcggttgcg cttggggccg ccgaagcaga
    18781 cgttgccgca gatctcgggc agccggatgc gcccgatgag ctggccctgc ggcgtccaca
    18841 ctgtcacccc gctgtagccc acgttgcggc ccgcgttgct cgagacccag agatttccgt
    18901 ccacgtcgga gcgcacgccg tccggcccgc acttgacgcc gtcgaccatg aagttgctga
    18961 agagcttgcc gttgctgagc ttgttgtccg cgcccacgtc gaacacgtac atctcgccct
    19021 tgccgcccgc gtgcgtgtcg cccggccccc tgccggtgct gaccacgtag agcttcttga
    19081 agtcgggcga gaagcagagc ccgttgggat ccggcaccag atcctcgccc gcgacctggg
    19141 tgagggcgcc gctcttgtcc acgcggtaca ccgcggtggg caactcgcgc ttgtagctgc
    19201 cgagctcggg cggctggccg agcttcggat tgagccggcc ggccatgttg gctggcccgc
    19261 ccggcgcgtc caccgtgccc tcgtagagct gggcgccgta gggcgggtcg gtgaaccagt
    19321 agctgccgtc cggatgcggc accacgtcgt tgggcgagtt gagccgcttg ccgttgaaca
    19381 tggacgcgat gatgctcgtc gacccgtcga gctcgtaacg caccacgcgc cgggtgacgt
    19441 gctcgcagga gagctggcgg ccctggaagt cgaacgtgtt gccgttgctg ttgttcgacg
    19501 gcgtgcggaa cacgctgacg tggccgttgt cctcgatcca ccggagctgc cggttgttcg
    19561 ggatatcgct ccacaccaga taccggccct gcgagctcca cgccggccct tccgaccaga
    19621 gcgcgccggt ccacagccgc tggatcgggg cattcggctg ccggaggccg ttgaacatgg
    19681 gctcgacggt gagcacgtcg ggatcggtga agtaggtggt gggcgccccg cgcgggctga
    19741 agtcacgcgg ggggctggtg atcgtgctgg gcggggccac cggcccgctg ggcggcgtct
    19801 gcgcgcccgc gagacgcggc gcgagcaccg cggcgcccac cgccgcgccc acgctgcgga
    19861 gcatcacgcg gcgcgatatc gaggtgccgg cttccggatc gtgcgcttct tgcgccattg
    19921 cgacctccct gagacgtcgt cgttcgagag ggaaacgtgg agaagcgatt cgcggggcaa
    19981 ccctagcaga cggccggagg agcgtcaaat cgcagcgtat gataggcgca gccgtgggga
    20041 ccccgggggg aggtggccaa tgagcaagcg aaagccgaag ccgaagcgga gccggacgaa
    20101 ggcgatgaaa ccgaaggcga cgaagaaggt cgtgaagaag gtcgcgaaga agaagggcgg
    20161 cgcggccccg gcccggcggg ccaggcgcgg cgggcgcaag cgcacgacgg ccacccgacg
    20221 cggccgggag ggcgcggagg agcagggccg aacggtcggc ctgctgcgcg aggtgctcga
    20281 gcaccacgag accgggcccg cggcgagtgc cggcgacctg gacgcggact ggcagcgcgc
    20341 gcagagctcg ggcgaggaag cggcgggcgg cagcgtgtcc acccccgacc aggaccgggt
    20401 cgatgacatc ggccacgcgc tgggtgtgga gcagccatcc acggcgccgc tccgctcctc
    20461 ggaggagatg ctggaagagc gcgaccggcg gtactgggag ctcgagcgcc gggcgcagga
//
LOCUS       DASUFA010000227         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1295752, whole genome shotgun sequence.
ACCESSION   DASUFA010000227 DASUFA010000000
VERSION     DASUFA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295752"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFX87_08420"
     CDS             complement(<1..1227)
                     /locus_tag="VFX87_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX4994977.1"
                     /translation="MDLSDSPRSLSWTSLVALTLLAAFAEAMILFPSTLFTPTRLAAA
                     LSWDASQHDYPSPETLAGETPATPEATTPVAPVTPASQMPAPASPAARRGDARAIAGR
                     VLDAATGSPLRGVSIWSGPTEIVTDAEGRFATGPVEAGATVWVKAPGYERKKLTGGEA
                     AEVTVKLAPHPIRAAYLTYYGVADRGIRQRVLDLVERTELNAVVIDIKGDRGLIPYRT
                     SVQAALDAGAQGPVIIKDFDEQLAGWKAQGIYTIARIVAFKDNVRANARPDLAVIDTR
                     TGKPWIDRENLAWVDPFREENWDYIIGVAKEAARKGFDEIQFDYVRFPTDGKLAAARY
                     SQPNSAATRLPAIAAFLAKARRELGPTGAFLAADIFGYTAFNANDTDIGQRIEEISVS
                     LDYICPMVYPSGYHLGI"
     gene            1456..2313
                     /locus_tag="VFX87_08425"
     CDS             1456..2313
                     /locus_tag="VFX87_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4994978.1"
                     /translation="MTDPIHFACETARPRALKVGGLTLNALEWGEPGRPALCFLHGGS
                     AHAHWFDGVVATFAGRFHVLSLDQRGHGASEWAPEPAYATEDFASDLVGVADAMGWPR
                     MTVIGHSMGGHNAIGFAAWYPDRTERLVVVDSRPSIPAERLQNMHRRGDRGPMRHETL
                     ESALKSFRLLPRETVADPRLLEHLARQGITERGGRFLYRFDPACNGRRRPTDGWSLLE
                     RITAPTLLVRGEHSPILPREMAAEMLTRLPLARLVEIAGTYHHLVLDAPLPFATVLDA
                     FLSEATAPA"
     gene            complement(2335..>3917)
                     /locus_tag="VFX87_08430"
     CDS             complement(2335..>3917)
                     /locus_tag="VFX87_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4994979.1"
                     /translation="EAGCLMVVGADASANHPVIAIRFRRAVAGGARLIVVNPKRVDLC
                     DQADLWIRQQPGTDVALFNAMARVILDEGLADLDFVRSRTEGFDAWVRSLEPYTLEHA
                     EAVTGVAREDIAQAARWYARPPFAGSCLIWGMGITQHINGTANAHAVLNLSLAAGQMG
                     FPGSGISPLRGQNNVQGAGDAGCIPTNLPGYAGYGAGDLDRFERAWGVRPPGEQGLVV
                     TEMVEGCLDGRVRAMYVVGENPLLSEPDLHHAEKAIQQLDFLVVQDLFMHETAEHAHV
                     FLPAAAFAEKDGTFTNSERRVQRIRQAIEPPGQARPDWWIACELAKRTARRLGTTVTG
                     FDYRHPSEIFDEMAGLWPSVRGMSYARLEAGGLQWPCPSPEHPGTRYLYGDSFPRGKG
                     RFVPVTQTATAAELPDPDYPFLLNTGRLLYHWHGGTMTRRVQGLLELAPRLEVAVHPS
                     DARRLGVGDGAVLRVVSRRGELTAYAHLTEAVRPGAVFVPFVKLADSAANFLTNAAFD
                     PSAKIPEYKVCAVRLERI"
BASE COUNT          561 a         1358 c         1430 g          568 t
ORIGIN      
        1 gatgcccagg tggtagcccg atgggtacac catggggcag atgtagtcga ggctcaccga
       61 gatctcctcg atacgctgcc cgatgtcggt gtcgttcgcg ttgaaggcgg tgtagccgaa
      121 gatgtcggcg gccaggaacg cgccggtcgg acccagctcg cgccgggcct tggccaggaa
      181 ggcggcgatg gcgggcaggc gcgtggccgc cgagttgggc tgggaatagc gcgcggcggc
      241 cagcttgccg tcggtgggga agcgcacgta gtcgaactgg atctcgtcga agcccttgcg
      301 cgccgcctcc ttcgccaccc cgatgatgta gtcccagttc tcctcgcgga agggatccac
      361 ccacgccagg ttctcgcggt cgatccacgg cttgccggta cgggtgtcga tgacggccag
      421 gtcgggccgg gcattggccc gcacgttgtc cttgaaggcc acgatccggg cgatggtgta
      481 gatgccctgc gctttccacc cggcgagctg ctcgtcgaag tccttgatga tgaccggccc
      541 ctgcgcgccc gcgtcgagtg cggcctggac cgacgtgcgg taggggatca ggccgcggtc
      601 gcccttgatg tcgatgacca ccgcgttgag ctcggtgcgc tccacgaggt cgagcacgcg
      661 ctgccggatg ccgcggtcgg ccacgccgta gtaggtgagg taggcggcgc ggatcggatg
      721 cggcgcgagc ttgacggtga cctccgcggc ctcgccgccg gtgagcttct tacgctcgta
      781 acccggcgcc ttcacccaga cggtggcgcc cgcctcgacc ggcccggtgg cgaagcggcc
      841 ctcggcgtcc gtcacgatct cggtggggcc cgaccagatg gagacccccc gcagcggaga
      901 gcccgtggcg gcgtccagca cgcggccggc gatcgcgcgc gcgtcgcccc ggcgcgcggc
      961 cggtgaggcg ggagcgggca tctgcgatgc cggggtcacc ggcgccaccg gcgtcgtggc
     1021 ctcgggcgtc gcgggcgtct cgccggccag cgtctccggc gacggatagt cgtgctggct
     1081 cgcgtcccac gagagcgcgg cggcgaggcg cgtcggcgtg aagagtgtcg aggggaacag
     1141 gatcatggcc tcggcgaacg cggccagcag cgtcagggcg accagcgagg tccaggagag
     1201 cgagcgggga gagtcgctaa ggtccattgg gcggtctcag atcattccgg taaggggttc
     1261 gtgcagccaa gcgaattgtg gatgtgcacg cgagttatga gcaacccgct tgccatttcc
     1321 ccgtcccggc aagtaactga aaacgtggcc cccctcgacc cggccccggc cggccggcgt
     1381 ccacctgctg acaaagtccg ccaggtgaca acgcggatgg tcacaaccgc gagggccctc
     1441 ggtacaatgc gacgcatgac cgacccgatc cactttgcct gtgaaaccgc ccggccccga
     1501 gccctcaagg tgggtgggct gaccctcaac gccctggaat ggggcgagcc cggccgcccg
     1561 gccctctgct tcctgcacgg gggctcggct cacgcccact ggttcgacgg ggtcgtggcc
     1621 accttcgccg gccgcttcca cgtgctctcg ctcgaccagc ggggccacgg agccagcgag
     1681 tgggcgccgg agccggccta cgccaccgag gacttcgcga gcgatctggt cggcgtggcc
     1741 gacgccatgg gctggccgcg gatgaccgtg atcggccact cgatgggtgg ccacaacgcc
     1801 atcggcttcg ccgcctggta tccggatcgg accgagcggc tggtcgtggt ggacagccgg
     1861 ccctcgatcc cggcggagcg actccagaac atgcaccgcc ggggcgatcg cgggcccatg
     1921 cgccacgaga cgctcgagtc cgcgctcaag agcttccgcc tgctgccgcg cgagacggtg
     1981 gccgacccgc ggctgctgga gcacctggcg cgccagggca tcaccgagcg cggcggccgc
     2041 ttcctctacc gcttcgaccc ggcgtgcaat ggccggcgcc gtcccacgga tggctggagc
     2101 ctgctcgagc gcatcaccgc gccgaccctg ctcgtgcgcg gcgagcactc gccgatcctg
     2161 cctcgcgaga tggcggcgga gatgctcaca cgcctgccgc tggcccgact ggtggagatt
     2221 gccggcacgt atcatcatct ggtgctcgat gcaccactgc ctttcgcgac agtgctggat
     2281 gcgttcctca gcgaagccac cgcgcccgcc tgagcgcccg ggaccgggag tctatcagat
     2341 ccgctccagg cggaccgcgc agaccttgta ttccgggatc ttggcggaag ggtcgaaggc
     2401 ggcgttggtc aggaagttgg cggccgagtc ggccagcttc acgaagggga cgaacaccgc
     2461 gcccggtcgc accgcctccg tcaggtgggc gtaggcggtc agctcccccc gccgggaaac
     2521 cacgcgcagg accgccccgt cgccgacacc caggcggcgc gcgtcgctcg ggtgcaccgc
     2581 gacctcgagc cgaggggcca gctcgagcag gccctggacc cggcgggtca tcgtgccacc
     2641 gtgccagtgg tagaggagcc ggccggtatt gagcaggaag gggtagtcgg gatcgggcag
     2701 ctccgccgcg gtggcggtct gggtcaccgg cacgaagcgg cctttcccgc gcggaaagga
     2761 gtcgccgtag aggtaccggg tccccggatg ctcgggactc gggcagggcc actgcagccc
     2821 gcccgcctcg agccgtgcgt aggacatgcc ccgcacgctc ggccagaggc ccgccatctc
     2881 gtcgaagatc tcggacgggt gccgatagtc gaagccggtc acggtggtcc cgaggcggcg
     2941 cgcggtgcgc ttggccagct cgcaggcgat ccaccagtcg gggcgcgcct ggcccggcgg
     3001 ctcgatggcc tggcggatcc gctgtacacg ccgctccgag ttggtgaagg tcccgtcctt
     3061 ctcggcgaac gcggcggcgg gcaggaacac gtgggcgtgc tcggcggtct cgtgcatgaa
     3121 gaggtcctgt accacgagga agtcgagctg ctggatggcc ttctcggcgt ggtggaggtc
     3181 gggctcggag agcagcgggt tctcgcccac tacatacatc gcccgcacgc gcccgtcgag
     3241 gcagccctcc accatctcgg tcacgacgag gccctgctcg cccggcggcc ggacgcccca
     3301 ggcccgctcg aagcggtcga ggtcgcccgc gccgtagccc gcgtagccgg gcaggttggt
     3361 cgggatgcag cccgcgtcac ctgcgccctg cacgttgttc tggccgcgga gcggggagat
     3421 cccggagccc gggaagccca tctggccggc ggccagcgag aggttgagca ccgcgtgggc
     3481 gttggcggtg ccgttgatgt gctgggtgat ccccatgccc cagatcaggc acgagcccgc
     3541 gaacggcggc cgcgcgtacc agcgggcggc ctgcgcgatg tcctcgcgcg ccacgccggt
     3601 gaccgcctcg gcgtgctcga gcgtgtaggg ctcgagcgag cggacccacg cgtcgaagcc
     3661 ctcggtgcgc gagcggacga agtcgagatc ggcgagcccc tcgtccagga tcacgcgcgc
     3721 catcgcgttg aagagggcca cgtcggtgcc cggctgctgg cgaatccaga ggtcggcctg
     3781 gtcgcagagg tccacgcgct tgggattcac cacgatgagg cgcgccccac ccgccaccgc
     3841 gcgacggaag cggatcgcga tgaccgggtg gttggcggag gcgtccgcgc ccaccaccat
     3901 gaggcacccc gcctcct
//
LOCUS       DASUFA010000228         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_734109, whole genome shotgun sequence.
ACCESSION   DASUFA010000228 DASUFA010000000
VERSION     DASUFA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734109"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1390)
                     /locus_tag="VFX87_08435"
     CDS             complement(83..1390)
                     /locus_tag="VFX87_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4994980.1"
                     /translation="MSKTDAIVPGGRGGARIPAEMTGHLITNVRILDGSGREPFAGAV
                     RVDENRIADVATGPVAARADERVIDGRGATLMPGLIEPHAHLSFADITSYEMTRLPLE
                     EHLLATLRNARTMLDAGYTSALSAAAAKPRLDVVVKREIEAGRFPGPRLLANGPEITV
                     TGGLGDSNLLHLPHHETPTFCWVADGPDAIRRVCRLLAREGVDLLKLNLSGDLGTSAW
                     SSELTPMTEAEVAAAMEVARARGLRVCAHCRSGDSVRMAVKHGIEVIYHANYCDEAAL
                     DALEAARDRVFVAPAIGITYNLTQANGRWGVTPERAQGFERELAATVETVARMRTRGI
                     RVLPGGDYGFVHTPHGTYARDLWLFVKVLGYSPMETLVAATRLGGEIMGHPGELGVVK
                     PGALADLLLVDGDPLADVAILQDPGAIRMIMKDGALHKAPPEA"
     gene            complement(1600..2115)
                     /locus_tag="VFX87_08440"
     CDS             complement(1600..2115)
                     /locus_tag="VFX87_08440"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEX4994981.1"
                     /translation="MPTNPGAEPPVDDAHPDHQPPVHSPGPSEMLPAPVSQGPAIEQP
                     AKLIRIAAMIREMLEDVRQSKPDEPGRRRLREVYERALAGLKEGVSKELQDELDTLTI
                     PLNTSSSESEIRLAQAQLVGWLEGLFQGIQAALWSQHMQARAEIEEMRRRRGLPPGPG
                     DGPPREPGQYL"
     gene            complement(2118..2525)
                     /locus_tag="VFX87_08445"
     CDS             complement(2118..2525)
                     /locus_tag="VFX87_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4994982.1"
                     /translation="MKTERKGFKQMVDEAKSRIKTISLEEAKSRLGSDRVVFVDLRDI
                     RELEREGMIPGAFHCPRGMLEFWVDPDSPYHKDVFASDKEFVFYCNGAWRSALATDVA
                     QQMGLETVVEMAGGFAEWKKKGYPVAEKPSKKG"
     gene            2564..3565
                     /locus_tag="VFX87_08450"
     CDS             2564..3565
                     /locus_tag="VFX87_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4994983.1"
                     /translation="MLNVLVTMRFDQSQLDRLRAVSPEILVSQADPETADYAKVDVLY
                     AGSPPRDLGRAPALRWVQLHMAGVNALADHPLYTKTAIPLTTTSGVHAATIAEYAVTM
                     LLALAHRVPRMVEWQGRGGWPPDEQRWPLFVPAEVRGATLGIVGYGSIGRELARIAKS
                     AFAMTVLACKRDPSRRADDGYALPGTGDPDGVLPDEWLGPAQLDSLLARSDVVVLCAP
                     LTPETRGMIGAKALARMKPSAFFINVGRGASVDEAALARALRERQIAGAAVDVFDREP
                     PPAGHPLYAVDNAIVSPHVSGFLPSYDEKCSVLFAENLRRFLAGAPLLNLVDRARGY"
     gene            complement(3562..>3909)
                     /locus_tag="VFX87_08455"
     CDS             complement(3562..>3909)
                     /locus_tag="VFX87_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994984.1"
                     /translation="VIWRAGAVDAAATAATRAALLAGRLRVRFTPVADLDADRGRLVR
                     LDARTAARLGIGPGAIVELVNPRGAPLRAWVDALMPGDGPRGEIAPSALAMLGLAEGG
                     LVELRPVHSGTLG"
BASE COUNT          532 a         1390 c         1400 g          587 t
ORIGIN      
        1 gtctttcttc ctcgagtaca gacgataccg ccccgacgga agcttgcgga tcatcggccg
       61 atcaggcgag cccgagcgcg gctcatgcct cggggggcgc cttgtggagg gcgccgtcct
      121 tcatgatcat gcggatcgcc ccggggtcct gcaggatcgc gacgtcggcg agcggatcgc
      181 cgtccaccag caggagatcg gccagcgcgc cgggcttcac cacgcccagc tcgccgggat
      241 ggcccatgat ctcgccaccc agcctggtgg cggccacgag cgtctccatc ggcgagtagc
      301 cgaggacctt cacgaagagc cagagatcgc gcgcgtaggt gccgtggggc gtgtggacga
      361 agccgtagtc gccgcccggc agcacccgga tgccgcgcgt gcgcatgcgc gcgacggtct
      421 ccacggtcgc ggccagctcc cgctcgaagc cctgcgcgcg ctccggggtc acgccccagc
      481 ggccgttggc ctgggtcagg ttgtaggtga tgccgatcgc gggcgccacg aagacgcgat
      541 cgcgcgcggc ctccagggcg tcgagcgcgg cctcgtcgca atagttggcg tggtagatga
      601 cctcgatgcc gtgcttgacg gccatgcgca ccgagtcgcc gctccggcag tgggcgcaca
      661 cgcggagccc gcgcgcccgc gcgacctcca tggccgcggc cacctcggcc tcggtcatgg
      721 gcgtcagctc cgacgaccag gccgaggtgc cgaggtcgcc ggagaggttg agcttgagga
      781 ggtccacgcc ttcgcgggcc aggagccggc agacgcggcg aatcgcgtcg gggccgtcgg
      841 cgacccagca gaaggtcggt gtctcgtggt gcggcaggtg gaggaggttg ctgtcgccga
      901 ggccgccggt caccgtgatc tcgggcccgt tggcgagcag gcgcggtccg gggaagcggc
      961 ccgcctcgat ctcccgcttg accaccacgt cgagccgcgg cttggccgcc gccgcggaga
     1021 gcgcgctcgt gtagcccgcg tcgagcatgg tgcgcgcgtt gcggagggtg gcgagcaggt
     1081 gctcttccag cggcaggcgc gtcatctcgt acgaggtgat gtcggcgaag ctcaggtggg
     1141 catgcggctc gatcaggccg ggcatcagcg tggcgccccg accatcgatg acgcgctcgt
     1201 cggcgcgcgc ggccaccggg cccgtcgcga catccgcgat ccggttctcg tccacgcgca
     1261 ccgcgcccgc gaacggctcg cggccgctgc cgtccaggat gcgcacgttc gtgatcaggt
     1321 ggccggtcat ctcggccggg attctagcac cgccgcggcc cccggggacg atggcatccg
     1381 tcttgctcat tcatgcggat ccatgaggag ggaccgatgc cgagctatct gatgagctgc
     1441 acccattgcg ggtacgaagt cgcgttttgg acggagccgg atgcgaaaac tgaaggagtt
     1501 cggcaccttc tgcagttccc ggctcacggc gtcaaggtga cgcccagtga ggacgccctg
     1561 atccgggatg aggaaagagt taccgcgtaa tcccgaaaac tagaggtact ggccgggctc
     1621 ccgagggggc ccgtcgccag gacccggggg cagcccgcgg cgccgccgca tctcctcgat
     1681 ctcggcccgc gcctgcatgt gctggctcca cagggcggcc tggatgccct ggaacaggcc
     1741 ctccagccag ccgacgagtt gggcctgggc cagccggatc tccgactcgc tcgagctcgt
     1801 gttcagcggg atcgtgagcg tatcgagctc gtcctgcagc tccttcgaca cgccctcctt
     1861 gaggcccgcg agggcccgct cgtagacctc gcgcagccgc cggcggccgg gctcgtccgg
     1921 cttggactgg cgcacgtcct ccagcatctc gcggatcatc gcggcgatgc ggatcagctt
     1981 ggcgggctgc tcgatcgccg gcccctgcga tacgggcgcg ggcagcatct ccgaaggccc
     2041 gggcgaatgc acgggcggct ggtgatccgg atgcgcgtcg tcgacgggcg gctcggctcc
     2101 cgggttggtc ggcatggcta gcccttcttg gagggcttct cggcgaccgg gtagcccttc
     2161 ttcttccact ccgcgaagcc gccggccatc tcgaccacgg tctcgaggcc catctgctgg
     2221 gccacgtcgg tggccagggc ggagcgccac gcgccgttgc agtagaagac gaactccttg
     2281 tcggacgcga agacgtcctt gtgatacggg ctgtccggat ccacccagaa ctcgagcatg
     2341 ccccgcgggc agtggaaggc gccggggatc atgccctcgc gctccagctc gcggatgtcc
     2401 cgaaggtcga caaacacgac gcggtcgctc ccgaggcggc tcttggcctc ctccaggctg
     2461 atcgtcttga tgcgggactt ggcctcgtcc accatctgct tgaatccctt gcgctctgtc
     2521 ttcatggcgc ctcctcgcga gcaggaagga tatcatagcg cccatgctca acgtcctcgt
     2581 gaccatgcgc ttcgatcaga gccagctcga ccgtctacgg gcggtctccc ccgagatcct
     2641 ggtgtcgcag gccgatccgg agaccgcgga ctatgccaag gtcgacgtgc tctacgcggg
     2701 ctccccgccg cgcgacctcg gccgggcgcc cgcgctgcgc tgggtgcagc tccatatggc
     2761 gggcgtgaat gccctcgcgg accatccgct ctacacgaag accgccatcc cgctcaccac
     2821 cacgagcggc gtgcatgcgg cgacgatcgc ggagtacgcg gtgaccatgc tgctggccct
     2881 ggcccaccgg gtgccgcgca tggtggagtg gcaggggcgc ggcggctggc cgccggacga
     2941 gcagcgctgg ccgctcttcg ttcccgccga ggtgcgcggc gcgaccctcg gcatcgtcgg
     3001 ctacggcagc atcggccgcg agctcgcgcg catcgcgaag tccgccttcg cgatgacggt
     3061 gctggcgtgc aagcgcgatc cctcgcgccg ggccgacgac ggttatgcac tgcccggcac
     3121 cggcgatccg gacggcgtcc tgcccgacga gtggctcggg cccgcgcagc tcgactccct
     3181 tctggcccgg tcggacgtgg tggtgctctg cgcgccgctc acgcccgaga cgcgcggcat
     3241 gatcggcgcg aaggcgctcg cccgcatgaa gccctccgcg ttcttcatca acgtggggcg
     3301 gggggcgagc gtggacgagg cggcgctagc ccgcgcgctc cgcgagcggc agatcgcggg
     3361 cgcggcggtc gacgtcttcg atcgggagcc gccgccggcc ggccatccgc tctacgcggt
     3421 ggacaacgcc atcgtgtcgc cgcacgtctc cggcttcctg ccgagctacg acgagaagtg
     3481 ctcggtgctg ttcgcggaga acctgcggcg cttcctggcc ggcgcgccgc tgctgaacct
     3541 ggtggaccgc gctcgcggct actagccgag cgtgcccgag tggacggggc ggagctccac
     3601 gagaccgcct tcggccagcc cgagcatggc gagcgcgctc ggtgcgatct cgccgcgcgg
     3661 cccgtcgccg ggcatgagcg cgtccaccca cgcgcgcagc ggggcgccgc gcgggttcac
     3721 cagctccacg atggcacccg gtccgatgcc caggcgcgcg gcggtccggg cgtccaggcg
     3781 cacgaggcgg ccccggtcgg cgtcgagatc cgccaccgga gtgaagcgca cgcggagccg
     3841 gccggcgagc agggccgcac gcgtggccgc ggtcgcggcg gcgtccaccg caccggcgcg
     3901 ccagatcac
//
LOCUS       DASUFA010000229        44471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_976295, whole genome shotgun sequence.
ACCESSION   DASUFA010000229 DASUFA010000000
VERSION     DASUFA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 44471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44471
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976295"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFX87_08460"
     CDS             complement(<1..567)
                     /locus_tag="VFX87_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4994985.1"
                     /translation="MKVSATVQMAAAALRTNRLRTALTALGVIIGVGAVVTMMAIGAG
                     AQARVAEQIRSLGSNLIIVLSGSITSGGVRLGSGSLLTITEDDARAIQRDVSSVEVAA
                     PTMRGGVQVVFGNSNWSTALLGVTPEYFQARDWGAVSGRLLTPEDVDGASKVVVLGQS
                     VAENLFGGTDPIDQTVRIKAVPFTVVGVL"
     gene            complement(564..1277)
                     /locus_tag="VFX87_08465"
     CDS             complement(564..1277)
                     /locus_tag="VFX87_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4994986.1"
                     /translation="MDAPLIETQGLHKEYPLGDHVVHALRGVSVTIAAGEMVAVMGPS
                     GSGKSTFMTILGCLDTPTAGRYLLEGRDVSSLGDDDLSEIRNHRIGFVFQQFNLLPRI
                     TAQANVEMPLLYGDIASRERRERAQRKLEVVGLQGRAAHRPAQLSGGEQQRVAIARAL
                     VNDPILLLADEPTGNLDTRTSEEIMAVVQALHRTGLTIVLVTHEPDIAQYAQRTLHFR
                     DGMLVGDEAVQDRRQARAA"
     gene            complement(1295..1498)
                     /locus_tag="VFX87_08470"
     CDS             complement(1295..1498)
                     /locus_tag="VFX87_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994987.1"
                     /translation="MTKLFTIGDETTHVIGDAQCPECLEEYPEPCPCGGLIHAASGEE
                     DKAGTEWPTTRCDQCGRSEEELD"
     gene            complement(1518..1955)
                     /locus_tag="VFX87_08475"
     CDS             complement(1518..1955)
                     /locus_tag="VFX87_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4994988.1"
                     /translation="MYKHMLIPTDGSALSEMAVRHGMALAKAAGARVTVLTVSLPFRT
                     FALDPVMVSDSPVQYEQECEALAEKALGTARTEAGMAGVACETLHVAHAQPYQAIVET
                     AQTKGCDLICMASHGRKGISAFVLGSETYKVLTHSKIPVLVCR"
     gene            complement(1962..2612)
                     /locus_tag="VFX87_08480"
     CDS             complement(1962..2612)
                     /locus_tag="VFX87_08480"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX4994989.1"
                     /translation="MLNEDRERMVATQIEARGITDPLVLVAMRAVPREQFVPAALARF
                     AYEDRPLPIGEGQTISQPYIVAVMTQAAGLKPGARALEIGTGSGYGAAVLSRIAAEVY
                     TVERVGLLAEEARDRLARLGYRTVHVLEGDGTLGWAEHAPYDAIVVTAAGPRVPGALL
                     SQLAAGGRLIMPVGSGSHHQRLVRVTRTETQEYRYEDLEEVAFVPLIGAEGWTEGG"
     gene            complement(2646..3086)
                     /locus_tag="VFX87_08485"
     CDS             complement(2646..3086)
                     /locus_tag="VFX87_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4994990.1"
                     /translation="MTRGLMPWTGFGNLKQDMDRLFDRLATAKWDDFPTLGDWSPSMD
                     ISETKDSLVVKVEVPGMEQKDIRVSLQENLLTITGEKKQEKEEKDERYHRVERSYGAF
                     TRGVRLPVGVDGSKVSATFKNGLLTVTLPKTPAAKGATIPIKSE"
     gene            3339..3761
                     /locus_tag="VFX87_08490"
     CDS             3339..3761
                     /locus_tag="VFX87_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4994991.1"
                     /translation="MLGKICTKPAVSASAQMTVDQAARAMRTKNVGALVVVNAGRPVG
                     MLTDRDVAIEVVAKGMDPETARVGDVMNKKPITIREDLGILDAVKVFAKTGVRRLPVV
                     TKSGVLVGVITVDDLIMLLGTELGHVAGALSAGLRRAS"
     gene            3794..6613
                     /locus_tag="VFX87_08495"
     CDS             3794..6613
                     /locus_tag="VFX87_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4994992.1"
                     /translation="MAISDAIGQDPGAIGPDVLTIVSTLVDELGGRPGDRPVGLDDRL
                     DRDLGIGSLERVELLVRLEQRFRVHLPDAVMAEAASPRDLATAIRGAEPAAREVLPRT
                     RPPLGEAAPPPVEARSLVEVLTWHAEATPDRVHVFLRDEDGRERPITYGALWRRARAI
                     AAGLRDRRVGRGDNVAIMLRTEEAFFATFFGVLLAGGVPVPVYPPFRRDLIEDYAQRQ
                     VGILENAGARAIITFPEAMRVATLLRSRVRSVRHIVTANDLVRAALASPSPAGPDEPA
                     LIQYTSGSTGAPKGVLLSHANLLANIRGIGDAIQIRPDDVAVSWLPLYHDMGLIGSWL
                     SALYFGIPIAILSPLAFLARPARWLQAIHAHRATVSPAPNFGFDLCAQRITDEEIQGL
                     DLSSWRLALNGSEPVSPETIERFTRRFSAWGFQSSAMCPVYGLAEASVALTIPEPGAG
                     PRIDMVAREPFQHAGRALAAPAGEPATLQFVSCGRPLPRHEVRVVDRKGRPLGEREEG
                     RIEFCGPSVTAGYWRNPTATRAARRDGWMDSGDLGYWADGELFITGRVKDLIKKTGRN
                     LYPQEVEDVVGAIPGIRKGCVAAFGVHDPSVGTERLVVVAETRRSGALEREGLRGAVM
                     EGVVAALGVPADVVVITGPHAVLKTSSGKVRRGATRAAYLQGVMGRPGRPIAVQWARL
                     LLAAVMARVREAASRVPALVGGAYLASLLLLTIPPLWLLVRLAPTGPAADRAVRLWCR
                     LILALSGCSLRLEGGDHLTDVAGPVILAANHASYLDVVVLLAALPLPFRFVAKHELVR
                     APLVGRVIRKVGHLTVQRGQATRSVADAGRITWALQAGFSVLVFAEGTFVRAPGILPF
                     RLGGFKAAVDTGRPVIPVTIRGAREILPAYAWLPEPGPITVTVSAPITAEEDGWRAMV
                     RLRDRTRAEIGRRSGEPAVDRSYTP"
     gene            6632..7201
                     /locus_tag="VFX87_08500"
     CDS             6632..7201
                     /locus_tag="VFX87_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX4994993.1"
                     /translation="MTNGRGTDHILLTTLGQNWWMVGARGLLACLFGLAVLLRPGLSL
                     NLLVATFGVYAVLDGAWAVASALWVSRISFAMWPVLFEGAVSLGVGWLALGWPLVPRD
                     AVRAIAAWGLLTGVLELVAAARLPRRSAGSWLLLTGGVFSLFLAVLILMLPHAVEPSV
                     ARAVGVYALLFGVLLVAAAVSLRSVFRAR"
     gene            complement(7209..9275)
                     /locus_tag="VFX87_08505"
     CDS             complement(7209..9275)
                     /locus_tag="VFX87_08505"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX4994994.1"
                     /translation="MRPGEDFFNLYNHQLIRAGVAIPTVRVADPAFNARQVSGLIGEA
                     AERRAVAVLFPELCLSAYSCEDLFHQEALLDECRSALQHVVDASRAWPLLAVVGVPLV
                     VDQLLYNCAVVLCRGQVVGIVPKTYLPNYREFYERRQFAPGDAAVRDVVDCCGQRGVP
                     FGSDLLFQLEEQPLATFHVEICEDLWVPVPPSSDAALAGATVLLNLSASNVTVAKADY
                     RHLLVGSQSGRCLAAYLYSGAGYGESTTDLAWDGHAVIYENGDLLAESRRFGYAPQLV
                     CAEVDLERLAQERMRQTSFAQAAERNRERLRRFRRVPVRLPLPRRGRLLPERGYERFP
                     YVPSSVERRDARCAEIYEIQAQGLVKRLQFADLQRVVIGVSGGLDSTQALLVAVRAMD
                     LLRHPRSHILAYTMPGFATSRRTLDQAHRLMSAIGVTGHEIDIRPSARQMLGDIGHPF
                     SRGEEVYDVTFENVQAGERTSHLFRLANLHQALVVGTGDLSELALGWSTYGVGDHMSH
                     YAVNASVPKTLIQHLIRWVAQNGRLGAQASDILFEILGTRISPELIPGEPGGAQPAQE
                     TETVVGPFELQDFNLYYTLRFGYPPSKVAFLAYSAWHDRSRGTWPDIDPAERHQYGIE
                     QIKTYLRIFLDRFFRLSQFKRSAIPNAPKVGSGGSLSPRGDYRAPSDGEASAWLRELE
                     RIPEQE"
     gene            complement(9304..11292)
                     /gene="ligA"
                     /locus_tag="VFX87_08510"
     CDS             complement(9304..11292)
                     /gene="ligA"
                     /locus_tag="VFX87_08510"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX4994995.1"
                     /translation="MARLRADIGRHDRLYYERARPEISDAEYDALARELRRLEAKFPD
                     LVRPGSPTRRVAGAPAGPFERVEHRVAMLSLDSVTSVAEVRAFERNVRRGLPGVSPGW
                     VVEPKVDGLGIALLYRRGRLVRGATRGDGRDGEDVTRNLRTIPSIPTRLRGPLGNLDE
                     LEVRGEVFMPRAAFARLNRELAERGEPPFANPRNAAAGSVRQKDPTVTARRPLAMFAY
                     QVSYAPDLGAASHWRVLAALAAAGFATNPRNRRTRRLGDALTYAEKLETRRDRLEYDA
                     DGIVIKVDDLDQQGRLGATGHHPRWAIALKFPARQATTVIRAIDVQVGKSGVLTPVAR
                     LEPVEVGGVVIRNVSLHNEDEIRRKDVRAGDTVLIERAGDVIPYVVQVITSRRPRGRR
                     PFRFPGRCPACGALTLRPLGEAYWRCVGSACPAQLKERLRHFASRHAMNIEHLGEATI
                     AQLVERGLVRDVADLYRLTDAQTGRLQGFGRKSAAKLVAAVAASRGRGLARVLTGLGI
                     RLVGVHVARLLAARYGRLDRLAAASVAELAALQGVGPAIAGSVARFLADGGNRRVLHR
                     LAAAGVRMAEPRTALAARPLVGRSFVLTGALTGLTRDEAAELLERLGARVTGSVSRTT
                     DYVVVGDRPGEKLGAARRLGVPTLTERAFLAMVRRRAA"
     gene            complement(11337..13088)
                     /gene="polX"
                     /locus_tag="VFX87_08515"
     CDS             complement(11337..13088)
                     /gene="polX"
                     /locus_tag="VFX87_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX4994996.1"
                     /translation="MKNFEVARQFELMADILELKGENAFRIRAYRRAAQNLESLSEDI
                     DTVARENRLEDVPGIGADLAGKIQEYLRTGRIKEIAAASKGIPRGVVALLNIPGVGPR
                     TARVLYEREHITGIDQLKKRARMGRLRGLPGIQAKTEQNILKGIRLVRGGQERMPLGR
                     ALPLGRELVHALEGLAAVERISLAGSIRRMKETVGDIDILVTSSKPAAVMRAFVTLPQ
                     AAEVLERGTTKGSIRHRDGIQVDLRVVDPACFGAALVYFTGSKQHNIHIRDIGVKKGL
                     KISEYGVFRASTGRRLVSATEAEVYAAVGLPCIPPELREDAGEIEAALEHDLPDLVEL
                     GEIRGDLHCHTNATDGHHTIDALVTAAERRGYRYVAVTDHSPSTRVAGGLTAAELAAH
                     VRKIRAVQARHPRITVLAGSECDIRPDGSLDYPDQVLAGLDLVIASVHTAFKQPRAEM
                     TRRICRALAHPRVHILGHPTGRLIGERDPYAVDIEEVLRTARRHGKAVEINAYPDRLD
                     LSDVHARRAHELGVLVAVSTDAHMLDHLGWMELGVATARRGWTETRQVVNTWPPQKLL
                     AWLRAERAAPVRRAHGR"
     gene            complement(13111..13557)
                     /locus_tag="VFX87_08520"
     CDS             complement(13111..13557)
                     /locus_tag="VFX87_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4994997.1"
                     /translation="MTKRILLALDRSPRADAVASIAAEPARGAHATVRLLHVAPEPSA
                     VHDGQGRVVVYADQESESLSSQGLDYLRELEAHFAGVPVESVVRFGDPVGLILDEALA
                     FGADLIVVGTAGRSGIGRVLLGSVAERVFARAPVPVMLVRPTTAAI"
     gene            complement(13570..13788)
                     /locus_tag="VFX87_08525"
     CDS             complement(13570..13788)
                     /locus_tag="VFX87_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4994998.1"
                     /translation="MARAKKARVRRAKESPRSPKAGRPPNPQAIPARVRRRQKSARAA
                     FRKDTTIHQAAQGPRAIWAATKPKIKKG"
     gene            complement(13845..14309)
                     /locus_tag="VFX87_08530"
     CDS             complement(13845..14309)
                     /locus_tag="VFX87_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4994999.1"
                     /translation="MKVKDVMTKHPITIDAEAPVGTALDLMRTKHIRHLPVLDDAETL
                     VGIITDRDLRHAALAPALDEYLSVRAHRRVRQLSETLENLRVKDVMTWAVLTTGPEAP
                     LTYAALIMFESRVGSLPVLEQGRLVGMLTEQDVLKGLLLGGKIAEFSEGYMW"
     gene            complement(14490..15848)
                     /locus_tag="VFX87_08535"
     CDS             complement(14490..15848)
                     /locus_tag="VFX87_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX4995000.1"
                     /translation="MKPTTPGPEQRLAQTSKPAEDALRLHPFYRGKMQVMPKCAIRGL
                     EDFAIWYSPGVAAPCRAIQADPELVYAHTNKGNSIAIVTDGTRVLGLGDIGPEAGLPV
                     MEGKAMLFKYLGGVDAVPICLATKDPDELVRTVKLLEPTFGGINLEDIAQPKCFRVLD
                     SLRRQMSIPVWHDDQQGTATALLAGLLNALKVVGKDLGRIRIVMVGIGAANVAGYRLL
                     TASGVEPGAIVACDRQGILHRGRHDIEADQERYPDKWRVCRDSNADGLAGGIAEALRG
                     ADVCAAFSASGPGIIKPEWVRGMARDAIVFACANPVPEIWPWEAKAAGARVVATGRGD
                     FPNQLNNSLVFPGLFRGVLDVRARTITDAMARAVARELAAFAEARTIGEEDILPRMDE
                     WEVFPRVAAAAGLAAQEEGVARLTKGREALHADAARIMRDAREAVDLLMREGLIAPPP
                     GP"
     gene            complement(15852..16586)
                     /locus_tag="VFX87_08540"
     CDS             complement(15852..16586)
                     /locus_tag="VFX87_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995001.1"
                     /translation="MSEQASCLAAESRQDPVAPSTAPRDVRIAGDAVPQMGPSWDINA
                     SASRRQMAGKADAIAWQPSCTSSGDLSRGRAHTLANARRGSRAARGSPRPSRGPAGSR
                     DKRLFRHVMVAVDLSQRNERPLETALALATEWGSRVTLCHVIQRVAGLAPGELAAFYR
                     TLVARSERQLRRAARLFTDRAIEARTEVRLGEPATEIVRAALRGAVDLVVMGSHKVTP
                     GKRARGWGTTSYKVGIFCQCSILLVK"
     gene            16594..16872
                     /locus_tag="VFX87_08545"
     CDS             16594..16872
                     /locus_tag="VFX87_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995002.1"
                     /translation="MARRREAAVEVTDMRVTIVLLGAAILLAGCATGRIAWDKPGITQ
                     AEREREENACLRAAIGTDGEGGLLLPYCIDREVYTQCMQARGYTVRPK"
     gene            16869..17474
                     /locus_tag="VFX87_08550"
     CDS             16869..17474
                     /locus_tag="VFX87_08550"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995003.1"
                     /translation="MRAGLALLCLLGVLAAIPAADGAGPGPTEQLRGAIDQILQVLED
                     PALKGSVRAADRHRAVRQITDEIFDFEETARRAMAQHWRSLTPAQRSEFVETFSDLIE
                     RAYMSKIELYSGEKIQYPGERVEGDVATVATRILTKKGAEVPIDYRMLKRGERWRVYD
                     VSIEGVSLVASYRTQFNNVIRTSSYDELLRKMRSRVEEMRQ"
     gene            17471..17908
                     /locus_tag="VFX87_08555"
     CDS             17471..17908
                     /locus_tag="VFX87_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995004.1"
                     /translation="MSASAAFHRILVPTDFSVPAERAWALAQRLARAADAELELVHVV
                     SGRASPLGRAGPASEAVRKWAIEELEDCAGKARAQGLRVRVTLRTGAAYREIVALARE
                     GEADLIVLATLGRGGIGRALLGSVADRVVRLAPCPVLTVPRTG"
     gene            complement(17913..18452)
                     /locus_tag="VFX87_08560"
     CDS             complement(17913..18452)
                     /locus_tag="VFX87_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX4995005.1"
                     /translation="MDHHGASRRRALRLGLVGLAGLLAPARRAAAADARSLALRSLHT
                     GEFVRATYWAAGRYVRDGLIEIDRLLRDHRSNTVHPIDRRLLDVLGALRDRLETREAF
                     EVISGYRSPATNALLVASTRGVAPTSLHVAGMAVDIRVPGRPLAAVRDAAKALGAGGV
                     GFYPDSNFVHVDVGRIRYW"
     gene            complement(18458..20170)
                     /locus_tag="VFX87_08565"
     CDS             complement(18458..20170)
                     /locus_tag="VFX87_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4995006.1"
                     /translation="MRRPTEWMLGRPLRRRGATVAAAVLVAVSIVGPVVEAAPASTSQ
                     RIRELLDRPDGPRIDGQSLDPRALARFYRPRDFAPAWGAGDGGSDRAALLIRALVTAE
                     THGLDPARYHLDAIRGRQASSNGGHTGELDLLLTAAFLDYARDVRAGRLPPGRRDQDW
                     GISAAPFDAVAALAQGVREPATFRARLASLPPPAKDYGRLVEALRRYREVAARSDWPL
                     VPPGAPLGPGDDDARLVAVRARLAAEDEIVSRSVTRFDERLEKAVRRFQARHGLAVDG
                     IVGEATVRALNVPASDRIRQITLNLERWRWLPRDLGARYVVVNAADATLRVVEDDRTV
                     LTSRVVVGDLQHPTPVVQARLDAVVLNPKWNVPSSIAAQEILPRLRENRRYLAENDIV
                     IMERQESDPFGLAVDWSVISADAFPFRLQQQPGPDNPLGRIKFDIPNRFDVYLHDTPT
                     RALFARPRRAASHGCIRVERADELAAHVLADGTGRWTPERLEEAIGGGASPRIPVVPP
                     LPVYILYWTAFVGPDGLVHFRDDVYGRDRRLGAALAAERSAPGPDLARTIGGCASSRE
                     EASG"
     gene            complement(20167..22590)
                     /gene="lon"
                     /locus_tag="VFX87_08570"
     CDS             complement(20167..22590)
                     /gene="lon"
                     /locus_tag="VFX87_08570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX4995007.1"
                     /translation="MAIQAPGPAGFVVPDVLPVLPLRDAVVFPLTAAPLMVGQPRSVR
                     LVDDVMRGNRLLALVAQRDPTPEPATPEALYRLGTVGMIHQLARGPDGSVRLMVQGIE
                     RIKILDWAGTEPYIVARVEAAPDQMVEGTEVDALRRAAADIFRRLVEASPELPDELGV
                     AAESFTDPRHLLYFVASLVPLDLPVRQELLEMDPVPAKLRRLVDLLQRELAVRELGRK
                     ITSETEERLSKKQREYYLREQLRSIQRELGEDREADGQVPELRRRIEEAGLPDEARRE
                     AERELGRLADLSPSSPEHGMIATYLEWMASLPWSKLDGGTIDIGRAREVLDQDHFDLE
                     KVKDRLLEYLAVKKLRQERGGPANRPAGAAGPGEPPREPILCFVGPPGVGKTSLGQSI
                     ARALGRKFVRMSLGGVRDEAEIRGHRRTYIGALPGRIIQGIRRVETRDPVFMLDEIDK
                     IGADWRGDPSSALLEVLDPAQNNTFVDNYLGVPFDLSQVLFIATANSLETIPGPLRDR
                     MEILTLSGYTDEEKIGIARQYLIPKQLAAHGLTPAELAFEPEAIRQIVRGHTREAGVR
                     SLERQIAAAARKVARRLAEGQSQAVRITADQVAEYLGRPRFFDEVAERTGRPGVATGL
                     AWTPTGGDVLFVEATMMPSSEERLVLTGMLGDVMRESAQAAVSYVRSNAEALDVDPKL
                     FEGRTIHVHVPAGAIPKDGPSAGVTIMTALASLATRRPVRGDLAMTGEITLRGKVLPV
                     GGIKEKVLAAHRAGIRAVILPARNERDVEDVPEELRRQLTFIFVDEADEVLRHALAAA
                     GAAVRSGAR"
     gene            complement(22590..23003)
                     /locus_tag="VFX87_08575"
     CDS             complement(22590..23003)
                     /locus_tag="VFX87_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4995008.1"
                     /translation="MRYRRLSYRYTMLVRSAPVWPLGDVWPSDRPRPLPRVGWWPDAD
                     TYETADAIEVLVELAGLDEDDFEVQLFEDVLVVEGHRRLPSCAEGAVYHAAGIRQGPF
                     HVEVVLPSAVDADRVEARYERGLLRITLPKRTGRG"
     gene            complement(23000..24361)
                     /locus_tag="VFX87_08580"
     CDS             complement(23000..24361)
                     /locus_tag="VFX87_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX4995009.1"
                     /translation="MPTRIVILGAAGRDFHNFNVAYRDDPDVHVVAFTQAQIPRLSDR
                     RYPASLAGARYPAGIPIEDEADLEAICRRETVDRVVFAYSDVPHAGVMHLASRALAAG
                     ADFALLGPRSTMLRARVPVIAISAIRTGCGKSQTARWLGRRLRERGRRVAVLRHPMPY
                     GDLERQRVQRFARREDLDAAGCSAEEREEYEPHLAAGHVVFAGVDYAAIVEQAEREAD
                     LIVWDGGNNDFPLLRPDLHIVMADALRPGQADGYHPGEAVLRMADVVVINKVDAAPAG
                     NVRAVIDEVRAINPRATIVRAASPVRLDDPAAVRGRRVLVVEDGPTLTHGGMAYGAGY
                     VAATAGGAAAIVDPRASAAPAVREVFARYPHIGPVLPAVGYDAGQLQALRETINRAVA
                     DVVVAATPLDLAALIALEKPVVRARYEFEDAGEPTLGSLVEAFLARGAEGWHNEPGVR
                     ETR"
     gene            complement(24366..24806)
                     /locus_tag="VFX87_08585"
     CDS             complement(24366..24806)
                     /locus_tag="VFX87_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4995010.1"
                     /translation="MNVGDVMTKHPLVVDLDTPVRVAADLMRSKGIRHLPVVDAGAHV
                     LGIVTDRDLRHAAFLPALAEQTGLAPHRVKAPRVRDVMTWSVVTTQADTALVRAALTM
                     FQRRIGSLPVVADGRLVGMLTEHDVFTGLKKASGIEDEAEFFLW"
     gene            complement(24803..25105)
                     /locus_tag="VFX87_08590"
     CDS             complement(24803..25105)
                     /locus_tag="VFX87_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX4995011.1"
                     /translation="MNKSDLVLEVARVTGLTGEVSEAVVSAVFEQITEALVRGERVEL
                     RGLGTFEVRQRAGHAGRNPKTGAPVEVPPRKVPFFRMGKELRAILNPQAAPEKVHA"
     gene            complement(25261..26256)
                     /locus_tag="VFX87_08595"
     CDS             complement(25261..26256)
                     /locus_tag="VFX87_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HEX4995012.1"
                     /translation="MTLLPGKLPPALLRRLLRFRGARDRRVVVGPAFGLDAAVVDLGP
                     QYLVLKSDPVTFTTEEVGWYAVHVNANDVAVMGARPAWFQSTIIVPPRTAAAVVTWIF
                     RDIDRAARGLGIAVTGGHTEISAAVKQPIVAGDMQGLVHRAGLVTAAGARAGDLVIMT
                     KAAGIEGTSILARRFAGAARKVLGTRARREAARFNHRPGISVVGAALLAARGGASAMH
                     DPTEGGVAAALFELATASGRRLVVDLDRIAVHPHTTRLCAHFGLRPLGLIGSGALLLA
                     IAARRAPALLGAFRRRQIPAAVIGAVTSGRGVDAYRGGRRVAFQWSPRDELTRLR"
     gene            complement(26253..26795)
                     /locus_tag="VFX87_08600"
     CDS             complement(26253..26795)
                     /locus_tag="VFX87_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEX4995013.1"
                     /translation="MPLAAPLVIGLLLTGCATIPGGPSVMVLPGSGKSFEQFQTDDFV
                     CRQWAGQEIGITPGQASAGGTAAGAAIGTVVGAAAGAAIGAAAGSPAAGAAVGAGAGL
                     LGGTAAGASRAEGSSEAVQYRYDMTYMQCMYAKGHQIPVARGSQPAYTPPPPPPPAAP
                     LPPGIPPPPAGAPPPPPPGS"
     gene            complement(26840..27265)
                     /locus_tag="VFX87_08605"
     CDS             complement(26840..27265)
                     /locus_tag="VFX87_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995014.1"
                     /translation="MIRPEGAGVNITRRVAILVVVAVGAWAIPTWSEGQERKYSGTVL
                     SVDRSVSVIVVGDMGPWRIKDGVTQVDRRTIRVTPSTEFVSVSRAIGPAPSGWVGDFV
                     ESVLPVWQVKPGDWVTVIVQTADKRPTAVRVSVWKPTEG"
     gene            27434..28528
                     /locus_tag="VFX87_08610"
     CDS             27434..28528
                     /locus_tag="VFX87_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX4995015.1"
                     /translation="MTHALTRRDLLQGAFALVGTGAAGAREADSSPRHAPSKGVYLSY
                     YGVGDRTIQGRVFGLLDRTELNTIVIDVKGDRGFIPYESRAPLARSAGAMGPVRVPDF
                     DALLAGLKAKGIHTVARVVVFKDDVLARYRPEWAVADMTTGSPWLDNERLAWIDPFHE
                     EAWAYPIAVAQEAALKGFDEIQFDYLRFPSEGRLGAARYARASTQQSRVQAIGRFLRQ
                     ARLALAPVGAALAIDVFGYTAFNPDDTGIGQRIEELAPLVDVLCPMAYPSAYHVGIPG
                     FRNPVAHPYEVVFETVQRIRGRSAQAPVQVRPWIQDFGDYAFDRRPFGVPEIRAQMRG
                     ALDGGASGWMLWNPGNHYTAGALDSRNGRD"
     gene            complement(28544..29659)
                     /locus_tag="VFX87_08615"
     CDS             complement(28544..29659)
                     /locus_tag="VFX87_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX4995016.1"
                     /translation="MKSRIEIVEVWGIPIRIHLSWLLVFGLVSWSLAAGYFPAEYPGW
                     TAGSYWAIGAVTALAFFASILVHELGHSWVARRNGIPIESITLFVFGGVARISREPET
                     PGVEFRMAIAGPVTSVVLAGLFAGAWQLAREVPLLAAPAIWLARINFLVAVFNLVPGF
                     PLDGGRLFRAAVWHVTGSLRRASRVASFTGQLVAFGLTGWGILTVMGGNVIGGMWLAF
                     IGWFLQNAAADSHAEANLREVLRDVTVAQAMTQDCRRIEPGASLERIVREEVLGAGRR
                     RFTVTEGGRLTGLLTLHEIKAVPQERWAAVTAGDVMTPAEKVTAVGPTVDLLTALKKM
                     DDANVAQMPVVTGSELVGMIGREQILHYVRVRGELGV"
     gene            complement(29680..30099)
                     /locus_tag="VFX87_08620"
     CDS             complement(29680..30099)
                     /locus_tag="VFX87_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4995017.1"
                     /translation="MVARELMTPDPVTVTPQASIAEVWDLMREADVRHVPVVQADALV
                     GMVSDRDLGRVDIARLLNVEGAGALRDELATPIVRLMSSAVIAVGPEADIGEVIELLL
                     EHRIGAVPVVQEETREVLGIISYVDVLRALSGVLNED"
     gene            30311..31333
                     /locus_tag="VFX87_08625"
     CDS             30311..31333
                     /locus_tag="VFX87_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4995018.1"
                     /translation="MTRRPVVLVTGAGGEVGHGLIHQLADLAVYDVLALDVQAVDDEV
                     ARRCAATRTGDILDRHLLDRLRSEFEISVVFHLAALLSTRAEFVPETAHEVNVQGTLN
                     LLRLAVDEARSLGHPVRFLFPSSIAVYGLPDLDTKRAAGKVVEPAWLSPNTMYGCNKL
                     YCEHLGRYFARHYRQLAAGEPSGVDFRAIRLPGLISAFTEPSGGTSDYAPEMIHAAAQ
                     HRPYACFVREDTRIPLMAMPDAVTALLALLEAPAASLTSPVYNVAAFSPSAGELADLV
                     RHAFPDARISFAPDGRRQAIVDSWPEDVDDTLARRDWGFRPAYDLRRTFEEYLVPNIT
                     RRYAPR"
     gene            31348..32553
                     /gene="kbl"
                     /locus_tag="VFX87_08630"
     CDS             31348..32553
                     /gene="kbl"
                     /locus_tag="VFX87_08630"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX4995019.1"
                     /translation="MYGDTRRRLQGELDDIRAAGLWKDERLVQSPQGPRVAVGGREVL
                     NFCANNYLGLASEPALIRAAQDALERWGFGLASVRFICGTQTIHKELEAAIARFVGME
                     DAVLYTSCFDANGGLFETLLDEPDVVISDALNHASIIDGIRLCRARRERYAHADMADL
                     ERILKASASARTRLVVTDGVFSMDGDVAPLAGICDLADRYGALVVVDDSHATGFFGPT
                     GRGTPEHCGVAGRVDIITSTLGKALGGASGGFTAGPAEIVALLRQRSRPYLFSNTLPP
                     ALVAAGLAGLRLLSESTERRDRLAANTRWFRRAMTEAGFGLRSGEHPIVPIMLGDARL
                     AHRMAADLFDEGLYVVGFSHPVVPRDQARIRVQISAAHEPAHLERAVAAFAKVGRAHG
                     VIRAIAPGP"
     gene            complement(32534..32980)
                     /locus_tag="VFX87_08635"
     CDS             complement(32534..32980)
                     /locus_tag="VFX87_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995020.1"
                     /translation="MAKIRTVMLASDFSAVSDAAFARAVELAKANRATLLVMHAVMLS
                     TASLGSAYVPPPTWDRIEAAMHAAAQKQIGALVAKARRAGVRAAGLVVAGNPYEAIVR
                     AARSKRADMLVLGTHGRTGLPRFFLGSVAARVLATAPCPVLTVRGR"
     gene            32864..33520
                     /locus_tag="VFX87_08640"
     CDS             32864..33520
                     /locus_tag="VFX87_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HEX4995021.1"
                     /translation="MHDQERRPIGLGQLHRARERGIRDRGEIGRQHHGSDLGHVRASS
                     ASLITLAPTLRRALQLLYFMAPAYAANMAPPLVRYWSGWNAPISRRWLGPHKTVVGFG
                     MGVLAAVLATFVQSRVAWDGALAAPESWLTLGLRFGLGAMAGDTAKSFFKRRAGIAAG
                     RPWIPWDQIDFVLGALALVRGAAALSWVDLATILVLSVIGHVLVNHLAYWLGIRDVRW
                     "
     gene            complement(33542..33985)
                     /locus_tag="VFX87_08645"
     CDS             complement(33542..33985)
                     /locus_tag="VFX87_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995022.1"
                     /translation="MYKHILIPTDGSALSEVAIRQGIAFARAIRAKVTALAVSPSFHA
                     FDVDPLMVGYTLSPEQYAASSAARAEEYLAVARVQAGIAGVPCETLHVTRDHVYDAII
                     DTATKKGCDLIFMASHGRRGISAFVLGSETYKVLSHSKIPVLVCR"
     gene            complement(34033..34434)
                     /locus_tag="VFX87_08650"
     CDS             complement(34033..34434)
                     /locus_tag="VFX87_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4995023.1"
                     /translation="MKSELDRWFDRLAELKWDDFPTLGDWAPSMDISETKDSVVVKIE
                     VPGMDQKDIEVSLQENLLTVKGEKKQEKEEKDERYHRVERSYGAFTRSARLPVGVDAS
                     KVSAAFKNGLLTVTLPKTPAAKGTMIPIKAE"
     gene            complement(34753..34929)
                     /locus_tag="VFX87_08655"
     CDS             complement(34753..34929)
                     /locus_tag="VFX87_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4995024.1"
                     /translation="MTWAVITVGPDADVRDAARIMHDRKLGALPVADEGRVVGVITAT
                     DVIRAVVGRGGERS"
     gene            complement(35214..35699)
                     /locus_tag="VFX87_08660"
     CDS             complement(35214..35699)
                     /locus_tag="VFX87_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995025.1"
                     /translation="MYKRALIPLDGSSVAETIIPFIVDIAGPLDMEVVLLRVVEPIAP
                     VVIEGARHVEIEDVEARRTDAEEYLAPIAVELRNKAVRVETRVRRGNPAEQIVAVARE
                     TAADLIAMSTHGRGGLGRLVFGSVAQAVLRHVEIPVFLMRATEAQVAKRVARDPVEKH
                     A"
     gene            complement(35711..36187)
                     /locus_tag="VFX87_08665"
     CDS             complement(35711..36187)
                     /locus_tag="VFX87_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995026.1"
                     /translation="MKTERILVPLDGSTLAESAIATAAEMVRPGAAATLVLVRAAEAH
                     TFPGADATEAQVKVIQEAETYLAGVTERLKKQGLANVETSVWYGPPAAAIVDAARARK
                     ADLIVMTTHGRGGLGRVILGSVAESVLRGTTTPILLLRASEAPIETPTGTGQARPA"
     gene            complement(36201..36653)
                     /locus_tag="VFX87_08670"
     CDS             complement(36201..36653)
                     /locus_tag="VFX87_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4995027.1"
                     /translation="MRVRDVMARPVMTFRQAMTVGTAVNAMRARNIRHAPVVDDKGRL
                     VGMVSDRDLRTVILEPALRDAFADLDRVLRSQTVKDVMTWGMVSVKPDTLLREAAGVI
                     HANKIGAVPVVEHGRVVGMLAVGDVLKAVIQMLDEGVISGPGRWGAEA"
     gene            complement(36979..37545)
                     /locus_tag="VFX87_08675"
     CDS             complement(36979..37545)
                     /locus_tag="VFX87_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX4995028.1"
                     /translation="MASTSTLPELGALAVHRGETELNCWSIGCAAGEEPYTLAVVWCL
                     GPGLRLPGLHLRILATDIEPENLARARRGCYARSSLKDLPAAYFELAFVPTAQEWCVG
                     EVYRDGVTFVEQDVRQAAPAGPFGLILCRNVAFTYFDEARQRNALRTIVERLAPGGAL
                     VVGRGETLPDLGPGLEIWSKRSGVYRRR"
     gene            37762..38898
                     /locus_tag="VFX87_08680"
     CDS             37762..38898
                     /locus_tag="VFX87_08680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX4995029.1"
                     /translation="MEGSAPTDRQAERLTVFLCGDVMTGRGIDQALPHPGHPLIHEPS
                     VRDAGQYVRLAEAANGPVPRPVGFSYIWGAALDELARVAPDARVINLETSITRSDEYW
                     PEKEIHYRMHPDNVQCLTAAQIDVCVLANNHVLDFGHLGLRETLDTLSQARLGTAGAG
                     DTLAQARAPAVVEISGKGRLVVFGFGDESSGIPSSWAAADDRPGIDVLPDLSEATATG
                     IVERIQAVKRARDVVVASIHWGSNWGYEVSRGQQRFARRLLDGGVDIVHGHSSHHVRP
                     IELYRGKLILYGCGDFLNDYEGIAGYEEFRDDLTLMYFATLQPETGRLVSLRMTPMHI
                     RNLRANRATPEDAEWLGHTLARVSRAFGARVELTADGTLALLSG"
     gene            complement(38920..39213)
                     /locus_tag="VFX87_08685"
     CDS             complement(38920..39213)
                     /locus_tag="VFX87_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX4995030.1"
                     /translation="MPTYVVLTRLTPEAVKTPGELKRLEKVVADHVRRDCPQAKWVAN
                     YAILGPYDYMDIFEAPDETIAAKVVMIIRSYGHAQTETWTAMPWERFEIVLPS"
     gene            40246..40680
                     /locus_tag="VFX87_08690"
     CDS             40246..40680
                     /locus_tag="VFX87_08690"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX4995031.1"
                     /translation="MLANRAWKELEMNKRDFLSRVQQAAGLQSPKEAERRSVAVLTAL
                     SHVLPDSEARRHFASQLPGFLKSRLAEEHTRGLLLTGDAFLQHLGHALGVHAPEAERT
                     LTSVFGVLRDAVSRGQVGELETHLPREIASLLEKKTPPSPPS"
     gene            40693..40866
                     /locus_tag="VFX87_08695"
     CDS             40693..40866
                     /locus_tag="VFX87_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995032.1"
                     /translation="MIKKINGKYVVLSETTGRRFGTYDTRAEAERRLRQIEFFKHLRS
                     RSTRPRAVSPGGR"
     gene            40868..41188
                     /locus_tag="VFX87_08700"
     CDS             40868..41188
                     /locus_tag="VFX87_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995033.1"
                     /translation="MNVRVRVQIAKGKLREYEGRLKQLEGRLTALRGRAEQTTGRARL
                     QLVRAERRVRTAMDASVERVEIAIKALQARLETTLRETRALSRGVQTGIRTGAKTYRR
                     LGRR"
     gene            complement(41204..42646)
                     /locus_tag="VFX87_08705"
     CDS             complement(41204..42646)
                     /locus_tag="VFX87_08705"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX4995034.1"
                     /translation="MTRERGVRGNRRPGTARGAFASGPLLTRDAALFADLYELTMAAS
                     YLREGMRGPATFSLFARNLPRGRSFLVAAGLEDVLNFLRDFRFSDDARSYLGSLDLFE
                     ASFLEFLRGLRFTGLVRAVPEGTVVFQDEPLLEITAPIIEAQLVETAVLNLVHSQTLL
                     ASKAARCVLAARGRPVSEFGLRRTHGIDAGMKAARCAFIAGVSTTSNVLAGLHYGIPL
                     TGTMAHSYVSAFPREIDAFRAFARAFPAQTILLLDTYDTVAAARKAVVVAKEMETRGQ
                     RLAGVRLDSGDLVGLSRQVRRLLDEAGLAYVKIVVSGGLDETSIGECLARGAPIDAFG
                     VGTRMDVSADQPYLDMAYKLVEYDGRPVLKSSAGKETWPAPKQVYRRLRSGEGFAGDV
                     VALHEEPAPPGTVEILLRSVMERGRLLAPHPALTAIRDYGAAQIASLPEDLRELRPGG
                     TYPVSFSERLVSLQRSLEAQVRAVTAEKAP"
     gene            complement(42643..>44471)
                     /locus_tag="VFX87_08710"
     CDS             complement(42643..>44471)
                     /locus_tag="VFX87_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX4995035.1"
                     /translation="VDTLARMFWRRVERSGDRPAQMVKRSGGWETLDWSLVGTQVREV
                     ALGLLALGLRAGDPVAILSRSRAEWVQADFAIFSAGGVTVPIYPSYTAEQVAYIVNDA
                     EARVLIVEDDAQLAKVLEVRGRLAGLGRIVVIEGHEGPDPTFLGWDALRRLGRDHAAT
                     FGAELAARVTGVGPKQVATIVYTSGTTGPPKGVVQTHDNHVAMLASVTQVAPARPGDV
                     HVLFLPLAHSFARLEAFMGVYLGLTTAFAESLDKLGDDIREVRPHFLVAVPRVFEKVH
                     ARIVADVERGSPLRRGLFAWAMRVGHEVAGRRSAGRPVPALLALRHRLAHRLVFARLQ
                     AALGGRLRFAVSGGAALPSEIAAFFHAAGILILEGYGLTESCPVLTFNRPDRFRFGSV
                     GQPVPGVEIRLAPDAEILARGPNIALCGYWKRPEESAETFGADGWLHTGDIGRVDEDG
                     FVFITDRKKDLIVTSGGINIPPQAIESLLRSDPFVSQALVYGDGRPYPVALVTVNAEA
                     AAALARSRGILWTDHARLSQHPEVVGRVERIIAEKNTHLQSYARIKRFAVLPVELTEE
                     AGEVTPTQKLRRKQIAEKYADVLASLYDPRAARGQSAPVESR"
BASE COUNT         6388 a        16213 c        15275 g         6595 t
ORIGIN      
        1 cagcactccg acgaccgtga agggcaccgc cttgatccgc acggtctggt cgatcggatc
       61 ggtgccgccg aacaggttct cggcgacgct ctgccccagc acgaccacct tgctcgcccc
      121 gtccacgtcc tccggcgtga ggagccggcc cgacacggcg ccccagtcgc gggcctgaaa
      181 gtactccgga gtcacgccca gcagcgcggt cgaccagttg ctgttcccga acacgacctg
      241 cacgcccccg cgcatcgtgg gcgccgcgac ctccacgctc gacacgtcgc gctggatcgc
      301 ccgggcgtcg tcctccgtga tcgtgagcag gctgcccgag ccgaggcgga ctcccccgga
      361 ggtgatcgag ccggagagca cgatgatcag gttcgacccg aggctccgga tctgctcggc
      421 cacgcgggcc tgcgcgccgg ccccgatggc catcatcgtc accaccgccc ccactccgat
      481 gatcaccccg agggcggtga gcgcggtgcg gagccggttg gtccggagcg cggccgccgc
      541 catctggacc gtggccgaga ccttcatgcc gcccgggcct gccgccggtc ttggaccgcc
      601 tcgtcgccca ccagcatccc gtcacggaag tgcagggtgc gctgcgcgta ctgcgcgatg
      661 tccggctcgt gcgtgaccag cacgatggtc agcccggtcc ggtgaagcgc ctggaccacc
      721 gccatgatct cctcgctggt ccgggtgtcg aggttgcccg tcggctcatc ggccaggagc
      781 aggatcggat cgttgacgag cgcgcgcgcg atggccaccc gctgctgctc gccccccgag
      841 agctgggcgg ggcggtgcgc ggcccggccc tgcagaccca ccacctcgag cttgcgctgc
      901 gcccgttccc ggcgctcgcg actcgcgatg tccccgtaga gcagcggcat ctcgacgttc
      961 gcctgggcgg tgatccgcgg cagcagattg aactgctgga acacgaagcc gatgcggtgg
     1021 ttgcggatct ccgacaggtc gtcgtcgccc aggctcgaca cgtcgcggcc ttccagcaga
     1081 tagcggcccg ccgtcggcgt atcgagacag ccgaggatcg tcatgaacgt ggacttcccg
     1141 gaaccggacg gacccatgac cgccaccatc tcaccggccg cgatcgtgac cgagaccccg
     1201 cggagcgcat ggacgacgtg gtcgccgagc gggtactcct tatggagtcc ctgcgtctcg
     1261 atcagcggag cgtccatcgg gtttcgccga cacgtcagtc gagctcttcc tcggagcggc
     1321 cgcactgatc gcatcgcgtg gtcggccatt ccgtcccggc cttgtcctct tcaccggacg
     1381 cggcatggat caggccgccg cacgggcacg gctccggata ctcctccagg cactcggggc
     1441 actgcgcgtc gccgatgacg tgcgtcgtct cgtcgccgat cgtgaatagc ttggtcatga
     1501 ctcctccggc tcgtgtctca ccggcacacc agcaccggga tcttgctgtg ggtgaggacc
     1561 ttgtaggtct cgctgccgag cacgaacgcg gatatcccct tgcgcccgtg cgaggccatg
     1621 cagatcagat cgcagccctt cgtctgcgcg gtctcgacga tcgcctggta tggctgggcg
     1681 tgggccacgt gcagcgtctc gcaggcgacg ccggccatgc cggcctccgt ccgggccgtc
     1741 ccgagggcct tctcggccag cgcctcgcac tcctgctcgt attgcaccgg actgtcgctc
     1801 accatcaccg gatcgagcgc gaaggtgcgg aaaggcagcg agacggtgag gacggtcacc
     1861 cgggcccccg ccgccttggc gagcgccatg ccgtggcgga ccgccatctc cgacagggcg
     1921 gatccgtccg tggggatcaa catgtgcttg tacatgggcg cctagcctcc ctcggtccag
     1981 ccctcggccc cgatcagggg gacgaacgcg acctcctcga gatcctcgta ccggtactct
     2041 tgcgtttcgg tacgggtcac acgcaccagc cgctggtgat gggagccgga cccgaccggc
     2101 atgatcagcc gtccgcctgc ggcgagctgg ctcaacagcg ccccgggcac ccgcgggccc
     2161 gccgccgtca ccacgatcgc gtcgtacggg gcgtgctcgg cccagcccag ggtgccgtcg
     2221 ccttcgagga cgtggacggt gcggtagccg agccgggcca gacggtcgcg cgcctcctcc
     2281 gccagcagcc ccacccgctc gaccgtgtac acttccgcgg cgatccggga caggaccgcg
     2341 gcgccatatc ccgacccggt tccgatctcg agcgcccgag cgcccggctt gagcccggcg
     2401 gcctgggtca tcaccgcgac gatgtacggc tgcgagatcg tctgcccctc gccgatcggc
     2461 agcggccggt cctcgtacgc gaaccgggcc agcgccgcgg gaacgaactg ctcccgcggg
     2521 acggcgcgca tggccaccag cacgagcggg tcggtgatgc cccgcgcctc gatctgggtc
     2581 gccaccatgc gctctcggtc ctcgttcagc atcggagcat cacctcccct tccgccggat
     2641 gcccgctact cgctcttgat cgggatcgtc gcgcccttcg ccgccggcgt cttgggcagc
     2701 gtcaccgtca gcagcccgtt cttgaacgtc gcggagacct tgctgccatc caccccgacc
     2761 ggcagccgca cgccgcgggt gaacgccccg taggaccgct ccacccggtg ataccgctcg
     2821 tccttctctt ccttctcctg tttcttctcc ccggtgatcg tcagcaggtt ctcctgcagg
     2881 gagacgcgga tgtccttctg ctccatgccc ggcacttcga ccttcaccac caggctgtcc
     2941 ttcgtctccg agatgtccat gctcggagac cagtcgccca gcgtcggaaa atcatcccac
     3001 ttggcggtgg cgagtcggtc gaacagccga tccatgtcct gcttcaggtt cccaaacccc
     3061 gtccacggca tgagtccacg cgtcatggtg tctcctcctg agagcgcttg gttcgacatc
     3121 ctgacggcac gaggtgcaac gcgagtgcca gccgggaacg cgcgccgatt cagcgggttg
     3181 ccgtcgtcgc agatcggggc cgccgaggcg cgaccgcctc ggtgtggccg gcccgcccca
     3241 accctgcggc gccggaacat cgattccgcg cggctttgga cgcggcactc gccttgcagt
     3301 ccacacgatc gcccctatct cactcaagga ggctcatcat gctcgggaag atctgcacca
     3361 agccggcggt tagcgcatcg gctcagatga ccgtggacca ggcggcgcgg gcgatgcgca
     3421 cgaagaacgt gggcgccctc gtggtcgtca acgcgggccg tccggtcgga atgctgaccg
     3481 accgggacgt ggccatcgag gtcgtcgcga aggggatgga tcccgagacg gcgcgggtcg
     3541 gcgacgtgat gaacaagaag cccatcacga tccgggagga cctgggcatc ctggatgccg
     3601 tcaaggtctt cgccaagacc ggcgtccgcc ggctcccggt ggtcaccaag agcggcgtcc
     3661 tggtgggggt gatcacggtg gacgacctga tcatgctcct cggcaccgag ctgggccacg
     3721 tggccggcgc cctctcggcc gggctccgac gggcctcgta ggactcgccg ctccacggcc
     3781 tggcccggac agcgtggcga tctcggacgc gatcgggcag gatccgggag cgatcgggcc
     3841 cgacgtcctg accatcgtct cgaccctggt cgatgagctc ggcggccgac ccggcgaccg
     3901 gccggtcggg ctcgacgatc gcctcgatcg cgacctgggc atcggcagtc tcgagcgtgt
     3961 cgagctgctg gttcgcctcg agcagcggtt ccgggtgcac ctgcccgacg cggtgatggc
     4021 ggaggccgcc agtccgcgcg atctcgccac ggccatccgg ggggcggagc cggccgcccg
     4081 ggaggtgttg cctcggaccc ggccgccgct cggcgaggcg gcgcccccgc cggtcgaggc
     4141 gcggtcgctg gtcgaggtgc tgacgtggca cgcggaggcc acgccggacc gtgtccacgt
     4201 cttcctgcgt gacgaggacg gccgggagcg acccatcacc tacggcgcgc tctggcgtcg
     4261 agcgcgcgcc atcgccgccg ggctccgcga tcgccgcgtc ggccgcggcg acaacgtcgc
     4321 gatcatgctc cggaccgagg aggcgttctt cgccacgttc ttcggcgtcc tcctcgccgg
     4381 aggcgtgccg gtgcccgtct atccgccgtt cagacgggac ctgatcgaag actacgcgca
     4441 gcggcaggtg ggcattctcg agaacgcggg cgcgcgggcc atcatcacgt ttccggaggc
     4501 catgcgggtg gcgacgctgc tgcgctcgcg ggtccgatcg gtccgtcaca tcgtgaccgc
     4561 gaacgacctg gttcgggcgg cgctcgcgtc cccctcaccg gcgggacccg acgagccggc
     4621 gctgatccag tacacctccg gcagcaccgg cgcgcccaag ggcgtcctgc tctcccacgc
     4681 caatctgctg gccaacatcc gcggcatcgg cgacgcgatc cagatacgcc ccgatgacgt
     4741 cgcggtgagc tggctgcccc tctaccacga catgggcctc atcgggtcct ggctcagcgc
     4801 cctctacttc ggcatcccga tcgccatcct gtccccgctc gccttcctcg cccggccggc
     4861 gcgctggctc caggccatcc acgcgcaccg ggcgacggtc tcccccgctc cgaacttcgg
     4921 cttcgacctc tgcgcgcagc ggatcaccga cgaggagata cagggactgg acctgtcctc
     4981 ctggcggctc gcgctcaacg gctcggagcc cgtgagcccg gagaccatcg agcggttcac
     5041 ccgaaggttc agcgcgtggg gattccagtc gagcgcgatg tgcccggtgt acggcctcgc
     5101 cgaggcttcg gtggcgctca ccatccctga gcccggagcg ggcccgcgga tcgacatggt
     5161 cgcgcgggag cccttccagc acgccggccg cgcgctggcc gcccccgccg gggagcctgc
     5221 aaccctgcaa ttcgtctcgt gcggccggcc cctgccgcgg cacgaggtgc gggtggtcga
     5281 tcggaaaggc cggccgctgg gcgagcgcga ggaagggcgg atcgagtttt gcggcccctc
     5341 ggtcaccgcg ggctactgga ggaatccgac cgcgacgcgc gcggcgcggc gagacggatg
     5401 gatggactcg ggtgacctgg gctactgggc cgacggcgag ctcttcatca ccgggcgggt
     5461 gaaggatctc atcaagaaga ccggccgcaa cctctatccg caggaggtcg aggacgtcgt
     5521 gggagcgatc ccgggcatcc ggaagggctg cgtggccgcc ttcggcgtgc acgacccgag
     5581 cgtgggcacc gagcgcctgg tggtcgtcgc cgagaccagg aggtccgggg ccctcgagcg
     5641 ggagggtctg cgcggggcgg tgatggaggg tgtggtggcc gcgctgggcg tgccggcgga
     5701 tgtcgtggtc atcaccggcc cgcatgccgt cctcaagacg tcgagcggca aggtgcggcg
     5761 cggcgccacg cgcgccgcct acctgcaggg cgtcatgggt cgcccgggcc gcccgatcgc
     5821 cgtgcaatgg gcgcggctgc tgctggccgc cgtgatggcg cgggtccggg aggcggccag
     5881 ccgggtgccc gcgctcgtgg gcggcgccta tctcgcctcg ctgctgctgc tcaccatccc
     5941 gccgctctgg ctgctcgtgc gcctggctcc gaccggtccg gccgccgacc gggcggtccg
     6001 gctctggtgt cggctgatcc tggcgctctc gggctgctcc ctccggctcg agggtggaga
     6061 tcacctcacc gacgtcgcgg gcccggtcat cctggccgcg aaccacgcca gctatctcga
     6121 cgtcgtcgtc ctcctcgccg cgctgccgct tcccttccgg ttcgtcgcca agcacgagct
     6181 ggtccgggct cccctggtgg gccgggtgat ccgcaaggtt ggacacttga cggtgcagcg
     6241 gggccaggcc acgcgcagcg tcgccgatgc cgggcgcatc acctgggcgc tccaggccgg
     6301 cttctcggtg ctggtcttcg ccgagggcac gttcgtgcgg gcgccgggga tcctcccgtt
     6361 ccgcctgggc ggcttcaagg cggcggtgga caccggccgc ccggtgatcc cggtgacgat
     6421 ccgcggagct cgtgagatcc tgcccgcgta cgcctggctg ccggagccgg gtcctatcac
     6481 ggtcacggtg agcgctccca tcacggccga agaagacgga tggcgcgcca tggtgcgcct
     6541 gcgcgaccgc acgcgtgcgg agatcggccg ccggtcgggc gagccggcgg tggaccgatc
     6601 ctacacaccc tgaccgaaac gggagaccat catgaccaac ggcagaggca cggaccatat
     6661 cctgctcacg acgctgggcc agaactggtg gatggtcggc gcgcgcggcc tgctcgcctg
     6721 cctgttcggg ctcgcggttc tgctccgacc gggcctgtcc ctcaacctgc tggtcgccac
     6781 cttcggcgtc tatgcggtgc tggacggcgc ctgggcggtc gcctccgcgc tctgggtctc
     6841 gcggatctcg ttcgcgatgt ggcccgtgct cttcgagggg gcggtcagcc tcggcgtggg
     6901 gtggctggcg ctcgggtggc ccctcgtgcc ccgggacgcg gtacgcgcca tcgcggcgtg
     6961 ggggctcctc accggggtcc tggagctcgt cgcggcggcc cggctgccgc gccggagtgc
     7021 cggatcctgg ctgctgctga ccggcggggt gttctccctc ttcctggccg tgctcatcct
     7081 catgctgccc cacgccgtgg agccgtccgt cgcccgggcg gtgggtgtct atgccctcct
     7141 cttcggcgtg ctgctcgtgg ccgccgccgt ctcgctccgg agcgtgttcc gggcccggtg
     7201 agccccgctc attcctgctc gggaatgcgc tcgagctcgc ggagccaggc cgacgcttcg
     7261 ccatcgctcg gggcgcgata gtcgccgcgg ggcgagagtg acccgccgga tccgaccttg
     7321 ggcgcgttcg ggatcgcgct ccgcttgaac tggctgaggc ggaagaaccg gtcgaggaag
     7381 atgcgcaggt aggtcttgat ctgctcgatc ccgtactgat ggcgctcggc cgggtcgatg
     7441 tcgggccagg tgccccggga ccggtcgtgc caggcagagt aggcgagaaa ggcgaccttc
     7501 gatggcggat agccgaagcg tagcgtgtag tagaggttga agtcctggag ctcgaatggc
     7561 ccgaccacgg tctccgtctc ctgcgcgggc tgcgccccgc cgggctcgcc ggggatcagc
     7621 tccgggctga tccgggtgcc gaggatctcg aacaggatgt cgctcgcctg ggcccccagg
     7681 cgcccgttct gggcgaccca gcgaatcagg tgctggatca gggtcttcgg aacgctggcg
     7741 ttgaccgcgt agtgtgacat gtggtcgccg acaccgtacg tgctccagcc gagcgcgagc
     7801 tcgctcagat cgccggtacc gacgaccagg gcctggtgga gattggccag gcggaagagg
     7861 tggctcgtcc gctcgcccgc ctgcacgttc tcgaaggtca cgtcgtaaac ctcctcgccc
     7921 cggctgaacg gatgcccgat gtctccgagc atctggcgcg cgctcggcct gatatcgatc
     7981 tcgtgccccg tgaccccgat cgcggacatc agccggtgcg cctggtcgag cgtgcgtcgg
     8041 ctggtggcga agcccggcat ggtgtaggcg aggatgtgac tccggggatg gcgcaggagg
     8101 tccatcgccc gcacggcgac gagaagggcc tgggtcgagt cgagcccccc cgagaccccg
     8161 atgacgacac gctggagatc ggcaaactgc agccgcttga ccagcccctg cgcctggatc
     8221 tcgtagatct cggcgcagcg ggcgtcacgt cgctcgacgc tcgagggcac gtacgggaag
     8281 cgttcgtacc cgcgctcggg cagcagccgc ccgcggcgcg gaagcggcag gcgtacggga
     8341 acccgtcgga agcgccggag ccgctcccgg ttccgttccg cggcctgggc gaagctcgtc
     8401 tggcgcatcc gctcctgagc cagccgctcg aggtcgacct ccgcgcagac gagctgcgga
     8461 gcgtagccga atcggcggga ctcggccaag agatctccgt tctcgtagat gaccgcatgg
     8521 ccatcccatg ccaggtcggt cgtggattcc ccgtagcccg cgcccgagta gaggtacgcg
     8581 gccagacacc ggccggactg gctccccacg agcaggtgcc ggtagtcggc cttggccacc
     8641 gtgacgttgg aggccgagag attgagcaga accgtggcgc ccgccagcgc ggcgtcggac
     8701 gacggcggca ccggcaccca gagatcttcg cagatctcca cgtggaaggt tgccagcggc
     8761 tgctcctcga gctggaacag cagatcggag ccgaacggca cgccccgctg accgcagcaa
     8821 tcgaccacat cgcgcaccgc ggcgtcaccc ggcgcgaact gtcgccgttc gtagaattcc
     8881 cggtagttgg gcaggtacgt cttggggacg atcccgacca cctgcccccg gcacagcacc
     8941 actgcgcagt tgtagagcag ctggtcgacg accaggggca cgccgaccac cgccaacagg
     9001 ggccaggcgc gcgaggcatc gacgacgtgc tgcagggcgc tccggcactc gtcgaggagc
     9061 gcctcctggt gaaagagatc ctcgcacgag tacgcggaga ggcagagctc ggggaacagc
     9121 accgcgacgg cccgccgctc cgccgcctcc ccgatcagac ccgacacctg ccgcgcgttg
     9181 aaagccgggt cggccacgcg cacggtgggg atggccacgc cggcccggat gagctggtgg
     9241 ttgtaaaggt tgaagaagtc ctcccctgga cgcacccggt ttctccccgc cctcgggctc
     9301 ggctcacgcc gcgcggcgtc tcaccatcgc gagaaacgcc cgctcggtca gcgtcggcac
     9361 gccgagccgc cgagccgccc cgagcttctc tcccggccga tcccccacca cgacgtagtc
     9421 cgtcgtccgt gacaccgacc cggtgacccg ggccccgagc cgctccagga gctcggccgc
     9481 ctcgtcgcgg gtgaggccgg tcagcgcacc cgtgagcacg aacgacctgc ccacgagcgg
     9541 gcgagccgcc agggccgtcc tcggctcggc catgcgcaca ccggccgcgg cgagccggtg
     9601 aagcacccgg cggttgcccc catcggcgag gaacctcgcg acggacccgg cgatcgcggg
     9661 gcccacgccc tggagcgccg cgagctcggc gacggacgcc gcggcgagac ggtcgaggcg
     9721 gccgtagcgg gccgccagca ggcgcgccac gtgaacgccc acgagtcgaa tccccagccc
     9781 ggtgagcacg cgtgcgaggc cgcggccgcg agacgcggcg accgcggcga ccagcttcgc
     9841 cgccgacttg cgcccgaacc cctggagtcg ccctgtctgt gcatccgtca gtcgatagag
     9901 gtccgcgacg tcgcgcacga ggccgcgctc cacgagctgg gcaatcgtcg cctccccgag
     9961 gtgctcgatg ttcatggcgt ggcgtgaggc aaagtggcgg agccgctcct tgagctgagc
    10021 cggacacgcg ctgccgacgc accgccagta cgcctcgccc aagggccgga gggtcagggc
    10081 gccacatgcc ggacagcggc ccggaaaccg gaagggccgt cggcctcgcg ggcggcggga
    10141 ggtgatgacc tgtaccacgt aggggatcac gtccccggcc cgctcgatca gcacggtgtc
    10201 accggcgcgc acgtccttcc ggcggatctc gtcctcgttg tggaggctca cgttccggat
    10261 cacgaccccg cccacctcga ccggttcgag ccgggccacc ggggtcagga ccccggactt
    10321 gccgacctgc acgtcgatgg cgcggatcac ggtggtcgcc tgcctggccg ggaacttgag
    10381 cgcgatggcc cagcggggat ggtggccggt cgccccgaga cgcccctgct ggtcgaggtc
    10441 gtcgaccttg atcacgatcc cgtcggcgtc gtactcgagg cgatcgcgcc gtgtctcgag
    10501 cttctcggcg tatgtgaggg cgtcgccgag ccggcgagtc cggcgattgc ggggattggt
    10561 ggcgaagccc gccgcggcga gcgccgcgag gacccgccag tggctcgccg cgccgaggtc
    10621 cggcgcatag ctgacctggt acgcgaacat cgcgagcggc cgccgtgcgg tgaccgtcgg
    10681 atccttctgg cggacggacc ccgccgcggc gttgcgcggg ttcgcgaacg gcggctcgcc
    10741 gcgctcggcc agctcgcggt tgagcctggc gaaggcggcg cggggcatga agacctcacc
    10801 ccgcacctcg agctcatcca gattgcccag cgggccgcgg agcctcgtcg ggatgctcgg
    10861 gatcgtccgg agattccgcg tcacgtcctc gccgtcccgt ccgtcgcccc gcgtcgcgcc
    10921 ccggaccagt cggcctcggc ggtagaggag cgcgatcccg aggccgtcca ctttcggctc
    10981 caccacccag ccgggtgaga caccgggcag gccccgccgc acgtttcgct cgaaggcccg
    11041 cacctcggct acggaggtga cgctgtcgag cgagagcatc gcgacgcgat gctcgacgcg
    11101 ctcgaagggc cccgcggggg ctcccgccac gcgccgggtg gggctgccgg gcctgacgag
    11161 atcgggaaac ttcgcctcca ggcggcggag ctctcgcgcg agcgcatcgt attcggcgtc
    11221 cgagatctcc ggccgagccc gctcgtagta gaggcggtcg tgccgcccga tgtccgcccg
    11281 caggcgggcc atccggcggg cggcggcggc acgatcagct ccacgagccc gagtcgtcat
    11341 cgaccgtgcg cgcgtcggac cggcgcggcg cgctcggcgc gcagccacgc caggagcttc
    11401 tgtggcggcc acgtgttcac gacctggcgc gtctcggtcc agccccgccg ggcggtggcc
    11461 acgcccagct ccatccagcc gaggtgatcg agcatgtgcg catcggtcga gaccgccacc
    11521 agcaccccca gctcgtgggc ccgccgggcg tggacgtcgc tgaggtccag ccggtcggga
    11581 taggcgttga tctccaccgc cttgccgtgt cgtcgtgcgg tgcggagcac ctcctcgatg
    11641 tccaccgcgt agggatcgcg ctcgccgatc agccggccgg tgggatgccc gagaatgtgc
    11701 acccgcgggt gagcaagggc gcggcagatc cggcgcgtca tctcggcccg cggctgcttg
    11761 aaggcggtat gcacggatgc gatgacgagg tcgagcccgg cgagcacctg gtcgggatag
    11821 tcgagggagc cgtcgggccg gatgtcgcac tcgctccccg ccagcaccgt gatccgcggg
    11881 tgccgggcct ggaccgcccg gatcttcctg acgtgcgcgg ccagctcggc ggcggtgagc
    11941 ccgcccgcga cgcgcgtgga cgggctgtga tcggtgaccg ccacgtaccg gtacccccgc
    12001 cgctcggccg ccgtcaccag cgcgtcgatc gtatggtgcc cgtccgtcgc gttggtgtgg
    12061 cagtgcagat cccctcggat ctcacccagc tcgacgaggt cggggaggtc gtgctcgagc
    12121 gcggcttcga tctccccggc atcctctctg agctcgggcg ggatgcacgg cagcccgacc
    12181 gccgcgtaga cttccgcctc ggtcgcgctc acgaggcgcc gaccggtcga ggcccggaac
    12241 accccgtatt cggagatctt gagccccttc ttcaccccga tgtcccggat gtggatgttg
    12301 tgctgcttcg acccggtgaa gtacacgagg gccgccccga agcacgcggg gtcgacgacc
    12361 cggaggtcga cctggatgcc gtcccggtgg cggatcgagc ctttcgtggt gccgcgctcg
    12421 agcacctccg cggcctgggg cagggtcacg aaggcccgca tgaccgcggc cggcttcgag
    12481 gacgtcacga ggatgtcgat gtcgccgacg gtctccttca tgcgccggat ggagcccgcg
    12541 aggctgatcc gctcgaccgc ggccagccct tcgagggcgt gcacgagctc acggccgagt
    12601 ggaagcgcgc ggccgagcgg catccgctcc tggcctccgc ggacgagccg gatgcccttg
    12661 aggatgttct gctcggtctt cgcctggatg ccgggcaggc ctcgaagccg gcccatgcgg
    12721 gcccgcttct tgagctgatc gatccccgtg atgtgctccc gctcgtagag caccctggcc
    12781 gtccggggcc cgaccccggg aatgttcagc agcgccacca ccccgcgggg aatccccttg
    12841 ctcgccgccg cgatctcctt gattctcccg gtccgcaggt actcctggat cttgcccgcg
    12901 aggtcggccc cgatcccggg aacgtcctcc aggcggttct cgcgggcgac cgtgtcgatg
    12961 tcctcgctga gcgactcgag gttctgcgcg gcgcggcggt aggcccggat gcggaacgcg
    13021 ttctcgccct tgagctccag gatgtccgcc atgagctcga actgccgcgc cacctcgaag
    13081 ttcttcattc gaggcccacc ggtctcgccg ctagatcgcc gcggtcgtcg gccgcacgag
    13141 catcacgggc acgggcgctc tggcgaagac ccgctcggcc acgctgccca gcagaacgcg
    13201 cccgattccg ctccgccccg cggtacccac cacgatcagg tcggccccga aggctagggc
    13261 ctcgtcgagg atcagcccga ccggatcgcc gaagcggacg acgctctcca ccggcacccc
    13321 ggcgaagtga gcctcgagct cgcggaggta gtcgaggccc tggctgctca ggctctccga
    13381 ctcctgatcc gcgtacacca cgacgcgacc ctgtccgtcg tgcaccgccg atggctcggg
    13441 cgccacgtgc agcaagcgca ccgtcgcgtg ggcgccgcgc gcgggctcgg ccgcgatgct
    13501 cgcgaccgcg tccgcgcgtg gcgaccggtc gagcgccagc aggattcgct tggtcatccc
    13561 cggcctcccc taccccttct tgatcttggg cttggtcgcg gcccagatcg cgcgcggccc
    13621 ttgcgccgcc tggtggatcg tcgtgtcctt gcggaacgcc gcccgggcac tcttctgccg
    13681 acgacggact cgggcgggga tcgcctgcgg gttgggcggc cggcccgcct tgggggagcg
    13741 cggactctcc ttcgcgcgcc tgacgcgcgc cttcttcgct ctggccatca tgtcctcctc
    13801 tttcgcgagc ctcgacgtgg tcagcgatcc gcgggccgct cgtcctacca catgtagcct
    13861 tcgctgaact cggcgatctt gcccccgagc aggaggccct tcagcacgtc ctgctcggtg
    13921 agcatcccga cgaggcgccc ctgctcgaga accggcaggc tccccacgcg gctctcgaac
    13981 atgatgagcg cggcgtacgt cagcggggcc tccggaccgg tcgtgagcac ggcccaggtc
    14041 atcacgtcct tcacccggag gttctccagc gtctcgctca gctgccggac tcgccggtga
    14101 gcccggaccg acaggtactc gtccagcgcc ggggccaggg ccgcgtggcg gaggtcgcga
    14161 tcggtgatga tgccgacgag cgtctcggca tcatccagga ccgggaggtg gcggatatgc
    14221 ttggtgcgca tgagatccag cgccgtgccc accggggcct ccgcgtcgat cgtgatcgga
    14281 tgcttggtca tgacgtcctt caccttcatc gcgtctcctt cggtccgatc gcccgcttcg
    14341 ggtggctcat gggaatggag ttgcaacggg cttgccagcc gccgggggcc gcgacacctt
    14401 gcgaaccggg acgttagcgc ccaaccgaaa gtctcgtctg ggtctcaccc tcctcagatg
    14461 aggcgctcgg gccccatgcc ccgccccggt cacgggccgg gcggtggagc gatcaggccc
    14521 tcccgcatga gcaggtcgac ggcttcgcgg gcatcacgca tgatccgggc cgcatcggcg
    14581 tggagcgcct ctcggccctt cgtcaggcgc gcgacgcctt cctcttgtgc ggcaaggccc
    14641 gccgcggccg ccacgcgcgg aaacacctcc cactcgtcca tgcgcggcag gatgtcctcc
    14701 tcaccgatcg ttcgcgcctc ggcgaaggcc gccagctccc gcgcgaccgc cctcgccatc
    14761 gcgtcggtga tggtccgggc ccgcacgtcg agcacccccc ggaagagccc gggaaagacc
    14821 agcgagttgt tgagctgatt cgggaaatcg ccccggccgg tcgcgaccac gcgtgcgccg
    14881 gcggccttgg cctcccacgg ccagatctcg gggacggggt tggcgcaggc gaagacgatg
    14941 gcatcccggg ccatgccgcg tacccactcg ggcttgatga tccccggccc cgacgccgag
    15001 aaggccgcgc agacgtcggc gccccgcaag gcctcggcga taccgcccgc cagcccgtcg
    15061 gcgttgctgt cccggcacac gcgccacttg tccgggtatc gctcctgatc cgcctcgatg
    15121 tcgtgccggc cccggtgcag gatgccctgg cggtcgcagg ccacgatggc gcccggctcg
    15181 acgccgctgg cggtgagcag acgatagccg gcgacgttgg ccgcgcctat cccgaccatc
    15241 acgatgcgga tccggccgag gtccttgccc acgaccttga gggcgttgag cagacccgcc
    15301 agcagcgcgg tggccgtgcc ctgctggtcg tcgtgccaga cgggaatgct catctgccgc
    15361 cggagcgagt cgagcacgcg gaagcacttg ggctgcgcga tgtcctccag gttgatgccc
    15421 ccgaaggtgg gctcgaggag cttgacggtt cgcaccagct cgtcgggatc cttcgtcgcc
    15481 aggcagatcg ggacggcatc gaccccgccg agatacttga agagcatcgc cttgccttcc
    15541 atcaccggca gccccgcctc gggtccgatg tcgcccagac ccagcacccg cgtgccgtcg
    15601 gtgacgatgg cgatggagtt gcccttgttg gtgtgcgcgt acaccagctc gggatcggcc
    15661 tggatcgccc ggcacggggc ggccaccccc ggcgagtacc agatcgcgaa gtcctcgagg
    15721 ccgcggatcg cgcacttcgg catgacctgc atcttgcccc ggtagaaggg atgcaggcgc
    15781 agcgcgtcct cggccggctt gctcgtctgc gccagccgct gctccgggcc gggcgtcgtc
    15841 ggtttcatcg gtcacttcac cagcaggatc gagcactggc agaagatgcc gaccttgtag
    15901 ctggtggtgc cccagccccg cgcccgcttg cccggcgtga ccttgtgcga gcccatgacc
    15961 accaggtcga cggccccgcg cagcgccgcc cggacgatct cggtcgcggg ctcgccgagg
    16021 cgcacttcgg tacgcgcctc gatcgcgcgg tcggtgaaca gccgggccgc ccgccgtagc
    16081 tggcgttccg agcgcgcgac gagcgtacgg tagaacgccg cgagctcgcc cggcgccagg
    16141 cctgccacgc gctggatcac gtggcacagc gtcacacggc tcccccattc cgtggccagc
    16201 gccagcgccg tctcgagcgg ccgctcgttg cgctggctca gatccacggc caccatgaca
    16261 tgccggaaca gccgcttgtc ccgcgagccg gccgggccgc ggctcggccg cggcgacccg
    16321 cgcgcggcgc ggctgcctct cctcgcgttg gccaacgtat gggccctccc tcggctgagg
    16381 tctccgctcg atgtgcaact cggctgccag gcgatcgcgt cagctttccc agccatttgc
    16441 cgcctggaag ccgaggcatt gatgtcccag ctggggccca tctggggcac ggcgtccccc
    16501 gcgatgcgga cgtcgcgcgg cgcggtgctc ggcgccacgg ggtcttgccg agactcggcc
    16561 gcgaggcacg aagcttgctc gctcatgcac cgaatggcgc gccgccgcga agcggcagtg
    16621 gaggtgacag atatgcgggt gacgatcgtg ctgctcggag cggcgatact gctggccggc
    16681 tgcgccacgg ggcgaatcgc ctgggacaag cccggcatca cgcaggcgga gagagagcgg
    16741 gaagagaacg cgtgcttgcg tgccgccata ggcaccgacg gggagggcgg gctgctgctt
    16801 ccctactgca tcgaccgcga ggtctacacc cagtgcatgc aggcgcgggg ctacaccgtt
    16861 cgacccaagt gagggccggt ctcgctctgc tctgcctgct cggggtgctg gccgcgatac
    16921 ccgcggccga tggcgccggc cccggcccga ccgagcagct caggggagcc atcgaccaga
    16981 tcctccaggt cctcgaggat cccgccctca agggtagcgt cagggcggcg gaccgccatc
    17041 gggccgtcag acagatcacg gacgagatct tcgacttcga ggagacggcg cgccgggcga
    17101 tggcccagca ctggcggtcg ctcacccccg cccagcgaag cgagttcgtc gagaccttca
    17161 gcgatctcat cgagcgcgcc tacatgtcga agatcgagct ctacagcggc gagaagatcc
    17221 agtaccccgg cgagcgggtg gagggcgacg tcgccaccgt ggcgaccagg atcctcacga
    17281 agaagggcgc ggaggtgccg atcgactacc gcatgctgaa gcgaggcgag cggtggcggg
    17341 tctacgacgt cagcatcgag ggggtgagcc tcgtcgccag ctaccgcacc cagttcaaca
    17401 atgtcatccg cacgtcctcg tacgacgagc tcctgaggaa gatgcggagc cgcgtcgagg
    17461 agatgcggca gtgagcgcct ccgccgcctt tcaccggatc ctggtcccga ccgatttctc
    17521 cgtgccggcc gagcgggcct gggccctggc ccagcggctg gcgcgggccg ccgacgccga
    17581 gctcgagctc gtccacgtgg tctccggacg cgcgtcgccg ctcggccgcg cgggccctgc
    17641 ctccgaagcc gtccggaagt gggccatcga ggagctcgag gactgcgccg gcaaggcgcg
    17701 cgcgcagggc ctccgggtcc gggtcacgct gcgcacgggg gcggcgtacc gggagatcgt
    17761 ggcgctggcc cgcgagggcg aggccgacct gatcgtcctg gccacgctcg gccgcggcgg
    17821 catcgggcgc gcgctcctgg ggagcgtcgc ggatcgggtc gtgcgcctgg cgccctgccc
    17881 ggtgctgacg gtcccccgga ccgggtgacg cgtcaccagt agcggatgcg cccgacgtcg
    17941 acgtggacga agttcgagtc cgggtagaag ccgactccgc cggcgccgag cgccttcgcg
    18001 gcgtcacgta ccgccgcgag cggtcggccc ggcacccgga tgtcgacggc catgcccgcg
    18061 acgtggaggc tcgtcggggc gaccccgcgg gtggacgcga cgagcagcgc attcgtcgcc
    18121 ggcgagcggt agccggagat gacctcgaag gcctcgcggg tctccaggcg gtcgcgaagc
    18181 gcgcccagca cgtcgaggag acggcggtcg atcgggtgta ccgtattgct ccggtggtcg
    18241 cggagcagcc ggtcgatttc gatcagcccg tcgcgcacgt accggccggc cgcccagtac
    18301 gtcgcgcgca cgaactcgcc cgtgtgcagg ctccgcaggg caaggctgcg cgcgtcggcc
    18361 gcggccgcgc gacgggccgg cgccagcagg ccggcgaggc ccaccaggcc cagccgcagg
    18421 gcgcgccgtc gggacgcgcc gtgatgatcc atgccgatca gcctgatgcc tcctctcggg
    18481 acgacgcaca gccaccgatt gtccgagcca gatccggccc gggcgcgctc cgctccgcgg
    18541 ccagcgcggc gccgaggcgg cggtcgcgcc cgtagacatc gtcccggaag tgcacgaggc
    18601 catccgggcc gacgaacgcc gtccagtaca gtatgtacac gggcagcggc ggcacaaccg
    18661 gaattcgcgg cgaggcgccg cccccgatgg cctcctcgag ccgctccggg gtccaccggc
    18721 cggtgccgtc cgccagcacg tgggccgcga gctcgtcggc ccgctccacc cggatgcacc
    18781 cgtggctggc cgcccgccgg ggccgcgcga acagcgcccg ggtcggggtg tcgtgcaggt
    18841 agacgtcgaa gcgattcggg atgtcgaact tgatccgccc cagcggattg tccggtccgg
    18901 gctgctgctg cagccggaac gggaaggcat ccgcggagat cacggaccag tcgaccgcga
    18961 ggccgaacgg gtcgctctcc tgccgctcca tgatgacgat gtcgttctcg gccaggtagc
    19021 gacgattctc gcgaagccgg ggcaggatct cctgggccgc gatcgagctc ggcacgttcc
    19081 acttcggatt cagcaccacc gcgtcgagac gggcctgcac caccggcgtc gggtgctgga
    19141 gatcgccgac gacgacgcgg gaggtcagca cggtgcggtc gtcctcgacc acccgcagcg
    19201 tggcatcggc cgcgttcacc acgacgtagc gcgccccgag atcgcgcggg agccagcgcc
    19261 agcgctccag gttcagggtg atctgccgga tccggtcgga ggccgggacg ttcagggcgc
    19321 ggacggtcgc ctcgcccacg attccgtcga cggcgagccc gtggcgcgct tgaaagcgcc
    19381 ggaccgcctt ctcgagccgc tcatcgaagc gcgtgacgga tcgcgacacg atctcatcct
    19441 cggccgcgag ccgagcccgg acggccacga gccgggcgtc gtcgtctccc ggcccgagcg
    19501 gtgcccctgg agggacgagc ggccagtcgc tccgggccgc cacctctcga taacgccgca
    19561 gcgcctcgac gaggcgcccg tagtccttcg ccggcggcgg cagcgacgcc agccgcgcac
    19621 gaaacgtcgc cggctcgcgg acgccctgtg ccagagccgc caccgcgtcg aacggggccg
    19681 cggagatccc ccagtcctgg tcccggcgcc cgggcggcag ccggccggcc cggacatcgc
    19741 gcgcgtagtc gagaaacgcg gcggtcagca gcaggtcgag ctcaccggta tgcccaccat
    19801 tcgagctcgc ctggcggcct cggatcgcgt cgagatggta gcgcgcgggg tcgagtccgt
    19861 gcgtctcggc ggtgaccagc gcccggatga gcagcgccgc gcgatcggac ccgccgtcgc
    19921 cagcgcccca cgccggggcg aaatcccgtg gccggtagaa tcgtgcgagc gcccgcggat
    19981 cgagcgactg cccgtcgatg cgcggcccgt ccggccgatc caggagctcc cggatgcgct
    20041 ggctcgtgga cgcgggggcc gcctccacca ccgggcccac gatcgagacg gcaacgagca
    20101 cggcggcggc gaccgtcgcc ccgcggcggc gaagcggccg cccgagcatc cactccgtcg
    20161 ggcgtctcac cgcgcgccgg agcgaaccgc ggcgccggcg gcggcgaggg cgtggcgcag
    20221 gacctcgtcc gcctcgtcga cgaagatgaa ggtgagctgg cgccgcagct cctccggcac
    20281 gtcctcgacg tcgcgctcgt tccgcgccgg caggatgacg gctcggatcc ccgcccggtg
    20341 cgcggccagg accttctcct tgatcccccc gaccgggagc accttgccgc gcagcgtgat
    20401 ctcgccggtc atcgcgaggt ctccgcgcac gggtcgacgg gtggcgagcg aggccagggc
    20461 cgtcatgatg gtgacgccgg ccgacggccc gtccttcggg atggccccgg ccggaacgtg
    20521 cacgtgaatc gttcgtccct cgaagagctt gggatcgacg tcgagcgcct cggcgttgga
    20581 ccgcacatag gacaccgcgg cctgcgcgct ttcgcgcatc acgtcaccca gcatgccggt
    20641 gagcaccagc cgctcttcgc tgctcggcat catcgtggcc tcgacgaaca gcacgtcacc
    20701 accggtcggc gtccaggcca ggcccgtggc gacgcccgga cggccggtgc gctcggccac
    20761 ctcgtcgaag aagcgcggcc ggccgaggta ctccgccacc tgatcggcgg tgatccggac
    20821 cgcctggctc tggccttccg ccagtcgccg ggccaccttc cgggccgcgg cggcgatctg
    20881 gcgctcgagg ctgcgcacgc cggcctcgcg ggtatggccc cgcacgatct gacggatggc
    20941 ctccggctcg aacgcgagct cggccggcgt gaggccgtgg gcggcgagct gcttcgggat
    21001 gagatactgc cgcgcgatcc cgatcttctc ctcgtccgtg taaccggaca gggtcaggat
    21061 ctccatgcgg tcgcgcagcg gaccgggaat ggtctccagc gagttcgcgg tggcgatgaa
    21121 caggacctgc gacaggtcga acggcacgcc gaggtagttg tcgacgaacg tgttgttctg
    21181 cgccggatcc agcacctcca gcagggcgga cgacgggtcg ccgcgccagt cggccccgat
    21241 cttgtcgatc tcgtcgagca tgaacaccgg atcgcgggtc tcgacccgac ggatcccctg
    21301 gatgatgcgg ccgggaagcg cgccgatgta cgtccgccgg tggccgcgga tctccgcctc
    21361 gtcccgcacc ccgccgaggg acatccgcac gaacttccgg ccgagcgcac gcgcgatgct
    21421 ctggccgagg ctggtcttgc ccaccccggg aggccccacg aagcagagga tgggctcgcg
    21481 gggcggctcc cccggcccgg cggcgcccgc ggggcggttc gcgggaccgc cgcgctcctg
    21541 acgaagcttc ttgaccgcca ggtactcgag gagacggtcc ttgaccttct ccaggtcgaa
    21601 gtgatcctgg tccaggacct cgcgggcccg gccgatgtcg atcgtcccgc cgtccagctt
    21661 gctccagggc aggctcgcca tccattcgag gtaggtggcg atcatgccgt gctcgggcga
    21721 ggatggcgag aggtccgcca gccggcccag ctctcgttcc gcctcgcgcc gcgcctcgtc
    21781 gggcagcccg gcctcctcga tgcgccgacg cagctcgggc acctggccgt ctgcctcccg
    21841 atcctcgccg agctcccgct ggatggagcg gagctgctcg cgcaggtagt attcgcgctg
    21901 cttcttggac agccgctcct cggtctcgct ggtgatcttc cgccccagct cccgcaccgc
    21961 gagctcgcgc tgcagcaggt ccacgagccg ccggagcttg gcgggtaccg gatccatctc
    22021 gagcagctcc tggcgcaccg gcagatcgag cggcaccagc gacgccacga agtacaggag
    22081 gtggcgggga tcggtgaagc tctccgccgc caccccgagc tcgtcgggca gctccgggga
    22141 ggcctccacc agccgacgga agatatcggc ggccgcccgg cgcagcgcat ccacctcggt
    22201 cccctcgacc atctgatccg gggccgcctc gacgcgcgcg acgatgtacg gctcggtacc
    22261 ggcccagtcg aggatcttga ttcgctcgat cccctgcacc atcaggcgga cgctgccgtc
    22321 aggcccccgc gcgagctggt gaatcattcc cacggtgccg agtcggtaga gcgcctcggg
    22381 cgtcgccggc tccggcgtgg gatcgcgctg cgccaccagc gccagcagcc ggttgccgcg
    22441 catcacgtcg tccacgagcc ggacggaccg cggctggccc accatcagcg gcgccgcggt
    22501 cagcgggaag accaccgcgt cccggagcgg caggaccggg agcacatccg gaacgacgaa
    22561 gccggcgggg ccgggcgcct gaatcgccat cagccgcgtc cggtgcgctt cggcagcgtg
    22621 atgcggagca ggccacgctc gtagcgcgcc tcgactcggt ccgcgtcgac cgcggacggc
    22681 agcaccacct ccacgtggaa cgggccctgc cggatgccgg ccgcgtggta gacggcgcct
    22741 tcggcgcacg aggggagccg tcggtgccct tcgacgacca gcacgtcctc gaagagctgg
    22801 acctcgaaat cgtcttcatc gagcccggca agctccacca ggacctcgat cgcatccgcc
    22861 gtctcgtacg tatccgcgtc gggccaccag ccgacccgcg gaagcgggcg cgggcgatcg
    22921 ctcggccaga cgtctccgag tggccacacc ggtgcggagc gcaccagcat cgtgtagcgg
    22981 tagctcaacc gccggtatct catcgcgtct cgcggactcc cggctcattg tgccagccct
    23041 cggcccctcg cgccaggaac gcctccacga gcgaccccag cgtgggctcc ccggcgtcct
    23101 cgaactcgta gcgcgcgcgc accaccggct tctcgagcgc gatcagcgcg gccagatcga
    23161 gcggggtcgc cgccaccacc acgtccgcga cggcccgatt gatcgtctcg cggagcgcct
    23221 ggagctggcc cgcgtcgtag ccgaccgccg ggagcacggg accgatgtgc gggtagcggg
    23281 caaagacttc ccgcacggcc ggcgcggcgg atgcccgcgg gtccacgatg gccgccgcgc
    23341 cgcccgcggt ggccgcgacg tagcccgccc cgtacgccat gccgccgtgc gtgagggtcg
    23401 ggccgtcctc caccaccagg acgcgccgcc cccgcaccgc ggcggggtcg tccagccgga
    23461 ccggagaggc cgcgcgcacg atcgtggccc gcggattgat cgcgcgcacc tcgtcgatga
    23521 ccgcccgcac gttaccggcg ggagccgcgt ccaccttgtt gatcacgacg acgtccgcca
    23581 tgcgcagcac cgcttctccc gggtggtagc cgtcggcctg cccgggccgg agggcgtcgg
    23641 ccatgacgat gtgcaggtcc ggccgcagca gcgggaaatc gttgttgccc ccgtcccaga
    23701 cgatgaggtc cgcctcgcgt tcggcctgct ccacgatcgc cgcgtagtcc actcccgcga
    23761 agacgacgtg tccggcggcc aggtgcggct cgtactcttc acgctcctcg gcggagcatc
    23821 ccgcggcgtc cagatcctcg cgccgcgcga accgctgcac gcgctgccgc tcgaggtcgc
    23881 cgtacggcat cgggtgccgc agcaccgcga ccctccgccc gcgctcgcgg agccgccggc
    23941 cgagccagcg cgcggtctga gacttgccgc acccggtccg gatggccgag atggcgatca
    24001 ccggcacgcg cgcccggagc atcgtgcttc gtgggccgag cagggcgaag tcggcgcccg
    24061 ccgcgagggc ccgggaggcc aggtgcatca cgcccgcgtg cgggacgtcg ctgtacgcga
    24121 acacgacgcg gtccaccgtc tcgcgccggc agatcgcttc caggtcggcc tcgtcctcga
    24181 tcggaatgcc cgccggatag cgcgccccgg cgagcgaggc gggatagcgg cggtcgctga
    24241 ggcgcgggat ctgggcctgg gtgaacgcca ccacgtgaac gtccgggtcg tcgcggtagg
    24301 ccacgttgaa gttatggaag tcgcgaccgg ccgcgcccag gatcacgatg cgggtgggca
    24361 tgccctcacc agaggaagaa ctcggcctcg tcctcgatgc ccgatgcctt cttgagcccg
    24421 gtgaacacgt cgtgctcggt cagcatgccc acgagccggc cgtccgcgac caccggcagg
    24481 ctgccgatcc gccgctggaa catcgtcagg gccgcccgga ccagcgcggt gtcggcctgc
    24541 gtcgtcacca ccgaccacgt catcacgtcg cggactctcg gcgccttgac ccggtgcggc
    24601 gccaggcccg tctgctccgc cagggcgggc aggaacgccg cgtgccgcag gtctcgatcc
    24661 gtgacgatcc cgagcacgtg ggcgcccgcg tcgacgaccg ggaggtggcg aattcccttg
    24721 ctccgcatca ggtccgccgc cacccgcacc ggagtgtcga gatcgaccac cagcgggtgc
    24781 ttcgtcatca cgtcgccgac gttcatgcgt ggaccttctc gggcgccgcc tgcggattca
    24841 ggatcgcccg cagctccttc cccatccgga agaacgggac cttccggggc gggacctcca
    24901 ccggcgcgcc ggtcttcggg ttccggccgg cgtgcccggc ccgctgcctc acctcgaacg
    24961 tgccgagacc gcgcagctcc acgcgctcgc ccctcaccag cgcctccgtg atctgctcga
    25021 acaccgcgct cacgaccgct tcgctcacct cgccggtgag accggtgacg cgggccactt
    25081 cgagaaccag gtcgctcttg ttcatggccg tcgatctcct gtgcgagaac tttgcaactc
    25141 tgctgccacg agccgcgccc ccggaacacc acgatcccga caggttgcgc cgcaggggcc
    25201 cgggttcgct ggggcgacgt caccccagcg gggcgcggcg tccccgcggc cggtgccgcg
    25261 ctaccggaga cgggtcagct cgtcccgggg cgaccattgg aaggccaccc gccgccctcc
    25321 tcgatacgca tcgacacccc ggccggacgt caccgcgccg atgaccgccg cggggatctg
    25381 ccggcgcctg aaggcgccga gcagcgccgg ggcgcgacgc gcggcaatcg ccaggagcaa
    25441 cgcgccggat ccgatcagtc ccagcggccg aaggccgaag tgggcgcaca gacgcgtggt
    25501 atgggggtgc accgcgattc ggtcgagatc cacgaccaac cggcggcccg acgcggtggc
    25561 gagctcgaag agggccgcgg ccacgccgcc ctcggtgggg tcgtgcatcg cgctcgcgcc
    25621 gcctcgcgcc gccagcagcg cggcgccgac cacggagatt ccggggcggt ggttgaagcg
    25681 cgcggcctcg cggcgggcgc gagtacccag gaccttgcga gccgcgccgg cgaatcgccg
    25741 cgcgagaatc gaggtgcctt cgatgccggc ggccttggtc atgatgacca gatctccggc
    25801 ccgggcgccg gccgcggtca cgagaccggc tcggtggacg agcccctgca tgtcgccggc
    25861 cacgatcggc tgcttgaccg ccgcggagat ctcggtgtgg ccgccggtga cggcgatacc
    25921 gagcccgcga gcggcgcggt cgatatcccg gaagatccat gtcaccaccg cggcggcggt
    25981 gcggggcggc acgatgatcg tggactggaa ccacgcgggc cgagctccca tgaccgcgac
    26041 gtcgttggca ttgacgtgta ccgcgtacca gccgacctcc tcggtggtga aggtgaccgg
    26101 atcgctcttc aggaccaggt actgcggccc gaggtcgacg accgcggcat ccagtccaaa
    26161 ggccggaccc acgaccaccc ggcgatcgcg agcgccccgg aaccggagca gccgccgcag
    26221 gagagcgggc ggcagcttgc cgggaagcag agtcatgagc caggcggcgg cggtggcggc
    26281 gctcccgctg gtggcggcgg aatgcccggc gggagcggag cggccggtgg tggcggcggt
    26341 ggcggcgtgt acgccggctg cgacccgcgc gcgaccggaa tctggtgacc cttcgcgtac
    26401 atgcattgca tgtaggtcat gtcgtagcgg tactgtaccg cctcgctgct gccctccgcc
    26461 cggctcgcgc cggccgccgt gccgccgagc aggccggcgc cagctcccac agccgccccg
    26521 gccgcaggac tacccgcggc cgccccgatc gccgcgcccg ccgctgcccc gaccaccgtc
    26581 ccgatggccg cgcccgccgc ggtgcctccc gccgaggcct ggcccggcgt gatcccgatc
    26641 tcctgcccgg cccactgacg gcagacgaaa tcgtccgtct ggaactgctc gaagctcttc
    26701 ccggagccgg gcagcaccat gacgctgggc ccgccgggta tcgtggcgca tcccgtcagc
    26761 agcaggccga tcacgagcgg agcagcgaga ggcatgtcgc gcacggctat ggcctcgggg
    26821 caggtcggta ccttcacgct catccctcgg tgggtttcca gacggagacc cggaccgccg
    26881 tcggccgctt gtcagcggtc tgcacgatca ccgtgaccca gtctcccggc ttcacttgcc
    26941 acacggggag cacgctctcg acgaaatccc cgacccagcc gctcggcgcc ggcccgatcg
    27001 cccgactgac gctcacgaac tcggtcgagg gcgtgacacg gatggtgcgg cggtcgacct
    27061 gggtcacacc gtccttgatc cgccacgggc ccatgtctcc gaccacgatc acgctcacgg
    27121 agcgatccac cgagaggacc gtgcccgagt acttcctctc ctggccctcg ctccaggtcg
    27181 gaattgccca cgcgccgacc gccacgacga caagaatcgc gacccgtctc gtgatgttca
    27241 cgcctgctcc ctccggccgg atcatggagc aagccgtgtg ccactggttg gatcctggaa
    27301 ccgcttgatt ccgtgcacgg cccgcttcgt ccgatgaacg tgatccagcc gcgtggggcg
    27361 cccaggtccc accggctctc ccacggtcgc cccccccgag gtcggcattg gccttgcatg
    27421 tgacgcgaca tcgatgaccc acgcgctgac ccgccgggat ctgcttcagg gagcgttcgc
    27481 gctcgtcggc acaggagcgg ccggcgcgcg tgaggcggat tccagcccgc gtcatgcacc
    27541 gtcgaagggc gtctacctct cctactatgg agtgggggac cgcaccatcc agggtcgagt
    27601 cttcgggttg ctcgaccgta cggagctcaa caccatcgtg atcgacgtga agggagaccg
    27661 aggcttcatc ccgtacgaga gccgggctcc cctggcccgg agtgccggcg cgatggggcc
    27721 ggtccgtgtg cccgacttcg atgcgctgct cgccgggctc aaagcgaagg gaatccatac
    27781 cgtcgcgcgc gtcgtggtgt tcaaggacga cgtgctggcc cgctaccgcc cggaatgggc
    27841 cgtcgccgac atgacgacgg gctcgccctg gctcgacaac gagcgcctgg cctggatcga
    27901 tcccttccac gaggaggcgt gggcgtatcc gatcgcggtg gcccaggagg ccgcactcaa
    27961 gggtttcgat gagatccagt tcgactactt gcggttcccg agtgagggca ggctcggggc
    28021 agcgcgctac gcgcgggcca gtacgcagca gagccgtgtg caggccatcg ggcggttcct
    28081 ccggcaggcc cggctggccc tggccccggt tggcgcggcg ctggccatcg atgtcttcgg
    28141 ctacacggcc ttcaacccgg acgacaccgg aatcgggcag cgcatcgagg agctggcgcc
    28201 gctcgtggac gtcctctgcc cgatggccta tccatccgcg taccacgtcg gcatcccggg
    28261 cttccgcaac cccgtcgcgc atccctacga ggtcgtcttc gagacggtgc agcgcattcg
    28321 cgggcggtcg gcgcaggcgc cggtccaggt ccggccctgg atccaggact tcggcgacta
    28381 cgccttcgac cgccgcccct tcggagtgcc ggaaattcgt gctcagatgc ggggcgcgct
    28441 cgacggggga gcgagcggct ggatgctctg gaatcccggc aaccactaca cggcgggcgc
    28501 gctcgactcc cgcaacgggc gggactagcc gggcggccgc gccctacacc ccgagctcgc
    28561 cgcgtacccg gacgtagtgc aggatctgct cccggccgat catcccgacg agctcgctgc
    28621 ccgtcaccac cggcatctgc gccacgttgg cgtcgtccat cttcttgagc gcggtgagca
    28681 gatccaccgt gggccccacc gcggtcacct tctccgccgg cgtcatgacg tcccccgcgg
    28741 tgaccgcggc ccagcgctcc tgcgggacgg ccttgatctc gtgcagcgtg agcagaccgg
    28801 tcagccgtcc gccttccgtc accgtgaagc ggcgccgtcc cgccccgagc acctcctcgc
    28861 ggacgatgcg ctcgaggctc gcgccaggct cgatgcgccg gcagtcctgc gtcatcgcct
    28921 gggccaccgt cacgtcgcgc agcacctccc gcagattcgc ctcggcgtgg ctgtcggccg
    28981 ccgcgttctg gaggaaccag ccgatgaagg cgagccacat gcccccgatc acgttgcccc
    29041 ccatcacggt caggatgccc cagccggtga ggccgaacgc cacgagctgg ccggtgaagg
    29101 aggcgacccg gctcgcccga cggaggcttc cggtaacgtg ccacaccgcc gcgcggaaga
    29161 ggcggccgcc gtccagcggg aagccgggga cgaggttgaa gaccgccacg agaaagttga
    29221 tgcgggccag ccagatcgcg ggcgccgcga gcagcggcac ctcccgggcc agctgccacg
    29281 ctcccgcgaa caggccggcc agcacgacgc tcgtcacggg tccggcgatc gccatccgga
    29341 actccacgcc cggcgtctcc ggctcgcggg agatccgggc cacgcccccg aacacgaaca
    29401 gggtgatgct ctcgatcggg atcccgtttc ggcgggccac ccacgagtgc ccgagctcgt
    29461 ggaccaggat ggacgcgaag aaggccagcg cggtgacggc tccgatcgcc cagtacgacc
    29521 ccgcggtcca gcccggatac tcggccggga agtagccggc ggccagggac cagctcacga
    29581 ggccgaagac gagcagccag ctcaagtgga tccggatcgg gattccccag acctcgacga
    29641 tctcgatccg gctcttcaag gcggcgccgc ctgttccgtt cagtcctcgt tcagcacgcc
    29701 cgacagcgct cttagcacgt ccacgtagct gatgatcccg agcacctcgc gggtctcctc
    29761 ctgtaccacc gggacggcgc cgatcctgtg ctcgagcagg agctcgatca cctcgccgat
    29821 atccgcctcc gggccgacgg cgatcaccgc cgagctcatg agccggacga tcggcgtggc
    29881 cagctcgtcc cggagcgcgc ccgctccctc gacattcagc agccgggcga tgtccacgcg
    29941 cccgaggtcg cgatcgctca ccatgcccac cagggcgtcg gcctggacga cggggacgtg
    30001 ccggacatcc gcctcgcgca tgaggtccca cacctcggca atgctcgcct gcggcgtcac
    30061 ggtcaccggg tccggggtca tcagctcgcg tgcaaccatg gtggcctcct gtgctccacg
    30121 tgacgcatgc agcaaggacg gtgccatacg ggtggccgag gattcccgct ctcgtcctcc
    30181 ggactgagcc ccgccgggcg ctcgcgagtg aggcgacggc tccccggatg aggcggcgga
    30241 gtcccgccat ggcccgtgcc cttgcgctgg ggcgccggcc ctgctatccg tcggggccag
    30301 gagagtcctg atgacacgca ggccggtggt cctggtgacc ggcgcggggg gcgaggtcgg
    30361 gcacggcctc atccatcagc tcgcggatct cgcggtctac gacgtgctgg ctctcgacgt
    30421 ccaggcggtg gacgacgagg tcgcgcggcg ctgcgcggcg acccgcaccg gggacatcct
    30481 cgaccggcac ctgctggacc ggctgcggag cgaattcgag atctcggtcg tcttccatct
    30541 ggccgcgctc ctctccacac gggcggaatt cgtgccggaa acggcccacg aggtcaacgt
    30601 gcagggcacg ctgaacctct tgcggctggc cgtcgacgag gcccgctcgc tcggccatcc
    30661 ggtccggttc ctcttcccga gctcgatcgc ggtctacggc ctgccggacc tcgacaccaa
    30721 gcgcgccgcc ggcaaggtgg tcgaacccgc ctggctgtcg ccgaacacga tgtacggctg
    30781 caacaagctc tactgcgagc acctcgggcg ctacttcgcg cgtcactacc ggcagctggc
    30841 ggcgggcgag ccgagcgggg tcgacttccg ggcgatccgt ctccccgggc tgatctccgc
    30901 cttcacggag ccgagcggcg gcacgagcga ctacgcgccg gagatgatcc acgcggccgc
    30961 tcagcatcgc ccgtacgcct gcttcgtccg ggaggacacc cgcatcccgc tcatggccat
    31021 gcccgacgcg gtcaccgccc tgctggcgct gctcgaggcg ccggccgcat cgctgaccag
    31081 cccggtctac aacgtcgccg ccttcagccc cagcgcgggc gagctggccg atctcgtgcg
    31141 acacgcgttt ccggatgccc ggatctcctt cgcgccggac gggcgccggc aggccatcgt
    31201 cgactcgtgg cccgaggacg tcgacgatac gctggctcgg cgcgactggg gcttccggcc
    31261 ggcctacgat ctcaggcgca cgttcgagga atacctcgtg ccgaacatca cgcggcggta
    31321 cgccccgcgc tgatcgggag atggctcatg tacggtgaca cccgacgacg acttcagggc
    31381 gagctggacg acatccgcgc ggcggggctc tggaaggacg agcggctcgt ccagagcccg
    31441 cagggccccc gcgtggccgt gggcggtcgg gaggtgctca acttctgcgc caacaactac
    31501 ctcggcctgg ccagcgagcc cgcgctgatc cgagccgccc aggacgccct ggaacgctgg
    31561 gggttcgggc tcgcgagcgt gcgcttcatc tgcgggacgc agacgatcca caaggagctc
    31621 gaggcggcca tcgcccgctt tgtgggcatg gaggacgcag tgctctacac ctcgtgcttc
    31681 gacgccaacg gcgggctctt cgagacgctg ctcgacgagc ccgacgtcgt catctcggac
    31741 gcgctgaacc acgcctcgat catcgacggc atccgcctct gccgggctcg gcgcgagcgt
    31801 tacgctcacg ccgacatggc ggatctcgag cggatcctga aggccagcgc atcggcgagg
    31861 acgcggctgg tcgtgaccga tggcgtgttc tccatggacg gcgacgtcgc gcccctggcg
    31921 ggcatctgcg atctggccga ccggtacggc gcgctcgtgg tcgtggacga cagccacgcg
    31981 accgggttct tcggtccgac cggccggggc acgcccgagc actgcggcgt ggccggtcgc
    32041 gtggacatca tcacctcgac gctcggcaag gcgctcggcg gcgcgagcgg cggcttcacc
    32101 gcgggcccgg ccgagatcgt ggcgcttctg cgccagcgct cccgcccgta cctcttctcg
    32161 aacaccctgc cgcccgcgct cgtcgccgcc gggctcgccg gtctccggct cctgtcggag
    32221 tccaccgagc ggcgcgaccg gctcgcggcc aacacccgct ggttccgccg cgccatgacc
    32281 gaggcgggct tcggcctccg ctccggcgag catccgatcg tgccgatcat gctcggcgat
    32341 gcgcggctcg cgcaccggat ggcggcggac ctcttcgacg agggcctcta cgtcgtgggc
    32401 ttctcccacc cggtggtgcc gcgggaccag gcgcgcatcc gcgtgcagat cagcgcggcc
    32461 cacgagccgg cgcacctcga gcgcgccgtg gccgcgttcg ccaaggtcgg ccgcgcacac
    32521 ggcgtcatcc gagctatcgc ccccggaccg tgagcaccgg acagggcgcg gtcgcgagga
    32581 cgcgcgccgc cacgctgccc aggaagaagc gcggcaaccc ggtgcggccg tgcgttccga
    32641 gcacgagcat gtccgcgcgc ttcgacctgg ccgcccggac gatggcctcg tacgggtttc
    32701 cggccacgac caggcccgcg gcgcggacgc ccgcgcgcct ggccttcgcc accagcgccc
    32761 cgatctgctt ctgcgccgcc gcgtgcatgg ccgcctcgat ccgatcccag gtcggcggcg
    32821 ggacgtacgc gctacccagg ctcgcagtgg agagcatcac cgcgtgcatg accaggagcg
    32881 tcgcccgatt ggccttggcc agctccaccg cgcgcgcgaa cgcggcatcc gagaccgcgg
    32941 agaaatcgga cgccagcatc acggttcgga tcttggccat gttcgggcct cctcggcctc
    33001 attgatcacc ctcgcgccca ctctccgccg cgccctccag ctcctctact tcatggcgcc
    33061 ggcgtacgcc gccaacatgg ccccaccgtt ggtccggtac tggagcgggt ggaacgcgcc
    33121 gatcagtcgc cggtggctcg gcccgcacaa gaccgtcgtg ggcttcggga tgggcgtcct
    33181 cgcggcggtc ctggcgacgt tcgtccagtc ccgcgtcgcc tgggacggcg cgctcgccgc
    33241 tcccgagtcc tggctgacgc tcgggctccg ctttggcctc ggcgccatgg ccggcgacac
    33301 cgcgaagagc ttcttcaagc gccgcgccgg aatcgccgcg ggccggccat ggattccgtg
    33361 ggaccagatc gacttcgtgc tgggcgcgct cgcgctggtg cggggtgcgg cggcgctgtc
    33421 atgggtcgat ctggcgacca tcctcgtgct cagcgtgatc ggacacgtcc tcgtcaacca
    33481 tctcgcctac tggctcggga tccgggatgt gaggtggtag ccgccgcccc cgacggggcg
    33541 ctcagcggca gaccagcacc gggatcttgc tgtggctgag caccttgtag gtctcgctgc
    33601 cgagcacgaa cgccgagatc cccctgcgac catgcgacgc catgaagatc agatcgcagc
    33661 ccttcttggt cgcggtgtcg atgatcgcgt cgtacacatg atctcgcgtc acgtgcaggg
    33721 tctcgcaggg gacaccggcg atccccgcct gtaccctcgc caccgcgagg tactcctcgg
    33781 cccgtgccgc cgagctcgcc gcgtattgct ccggcgacag cgtgtagccc accatgagcg
    33841 gatcgacatc gaaggcgtgg aacgacggcg agaccgcgag cgcggtcacc ttggcgcgga
    33901 tcgcccgggc gaacgcgatt ccctggcgga tcgccacctc ggagagggcg gacccgtccg
    33961 tgggaatcag aatgtgcttg tacatgagac ctgcccttcc tcggtcccgc cgtcgctccc
    34021 gccggcttcc acctactcgg ccttgatcgg gatcatcgtg cccttcgcgg ccggcgtctt
    34081 cggcagcgtc accgtcagca gcccgttctt gaacgcggcc gagaccttgc tcgcgtccac
    34141 cccgaccggc agccgtgcgc tccgggtgaa ggccccgtag gaccgctcga cccgatgata
    34201 ccgctcatcc ttctcttcct tctcctgctt cttctcgcct ttgaccgtca gcaggttctc
    34261 ctgcagcgag acctcgatgt ccttctggtc catcccgggc acctcgatct tcacgaccac
    34321 gctgtccttc gtctccgaga tgtccatgct cggagcccag tcgcccagcg tcggaaaatc
    34381 atcccacttg agctccgcga ggcgatcgaa ccagcggtcc agctcgctct tcatacctga
    34441 catccccgtc cacggcatca gtccacgcgt catgaccgtt tcctccttct ggtgagagtg
    34501 tttcgatgag caccgtcatc gccaagatgt gcatcggcaa tgccagtcag aacatccagt
    34561 gattcaagac cttgcgatcg gcgggtggac gacggccggg gcgcccgcgc ccccgtgtgg
    34621 ccgcggcgcc ccaagggcga tctccggctt gaagtgcttg cagcggcacc cggccgcccg
    34681 gcattcgccc gtctggtcgt gttcccggtc ggagtgtcca cagatgcaca tgtccatgct
    34741 cgtcccctcg tgctatgacc gctccccgcc cctgcccacg accgcgcgga tgacgtccgt
    34801 ggcggtgatc accccgacca cccggccctc gtcggccacc ggcagggcgc caagcttgcg
    34861 atcgtgcatg atccgggcgg cgtcgcggac gtcggcgtcg gggcccacgg tgatgacggc
    34921 ccaggtcatg atctcgttcc accctcaggc cggcaagcgt gcgcgacccc tggctcggct
    34981 cctcccgaag ccaccgctcc aggatcacca gccggagatc atgatcggtg accatcccga
    35041 tgagccgccg gtccgggtcg agcaccggca gatggcggac ctgtcgcttc tgcatgatgc
    35101 tccaggcatc cgcaaccgtc gcgtcgtgtc cgaccgtcac cagggaatcg gccatcaagt
    35161 cggcgacccg catcgcggcc tccggatccg caggtgcagc agagatgccc ccctcatgca
    35221 tgcttctcca ccggatcccg ggcgacgcgc ttggccacct gggcttccgt ggcccgcatc
    35281 aggaacaccg gtatctcgac atggcgaagc acggcctggg cgaccgagcc gaacacgagc
    35341 cggcccagac cgccgcggcc gtgggtggac atcgcgatga ggtcggcggc ggtttcacgg
    35401 gcgaccgcca cgatctgctc ggccggattg cctcggcgta cccgggtctc gaccctcacg
    35461 gccttgttcc gcagctccac ggcgatcggc gccaggtact cctcggcgtc cgtgcgccgc
    35521 gcctcgacgt cctcgatctc gacgtgccga gccccctcga tcaccacggg cgcgatcggc
    35581 tcgacaaccc ggagcaggac cacctccatg tcgagtgggc cggcgatgtc cacgatgaac
    35641 ggaatgatgg tctcggccac cgacgagccg tcgagcggga tcagggcgcg cttgtacatg
    35701 gagcttctcc ttaggcgggt cgggcctggc ccgtcccggt gggggtctcg atcggtgcct
    35761 cggaggcgcg cagcagcagg atcggcgtgg tcgtcccgcg cagcaccgac tcggccacgc
    35821 tgcccagaat cacccggccc aggccgccgc gcccatgtgt ggtcatcacg atcaggtctg
    35881 ccttgcgcgc tcgcgccgcg tccacgatgg cggcggccgg gggcccgtac cacacgctgg
    35941 tctcaacgtt ggcgaggccc tgtttcttga gacgctcggt cacgccggcc agataggtct
    36001 cggcttcctg gatcaccttc acctgggcct cggtggcatc cgccccgggg aaggtgtgcg
    36061 cttccgcggc gcgcaccagt accagggtgg cggcggcccc gggacggacc atctccgccg
    36121 cggtggcgat cgcggattcg gccagcgtgg acccgtcgag cgggaccagg attctctccg
    36181 tcttcatgga ttccctttct tcaggcctcg gcgccccagc ggccgggccc ggaaatcacg
    36241 ccttcgtcga gcatctggat caccgccttc agcacatcgc cgaccgcgag cataccgacc
    36301 acgcggccgt gctccacgac cggcacggcc ccgatcttgt tggcatggat cacgccggcc
    36361 gcctcgcgga gcagggtatc cggcttcacg ctgaccatcc cccaggtcat cacgtccttg
    36421 acggtctggc tcctgagcac gcggtccaga tccgcgaacg cgtcgcggag ggcgggctcg
    36481 agaatgaccg tgcggaggtc gcggtcgctc accatgccga cgagacgccc cttgtcgtcg
    36541 accaccggcg cgtgccggat gttccgggcc cgcatggcgt tcacggcggt gccgaccgtc
    36601 atcgcctggc ggaacgtcat caccgggcgc gccatgacgt cgcggactcg caccacacct
    36661 tccggccagg aagactcctc gagctcgcgg cgcgctcggg ccgggcgctt cggattccgg
    36721 gtcctgatat ctcgggtgat ggctcgcttc gggtgtttca tgaggtcccg cctccgtttg
    36781 tcggctcgtt ccgacgtaac ctggagtgca acccccctgc caactgttgg cggccgtgac
    36841 cacttctgaa acacgacatt gccgccctgc ggccgggcgc cgctgaggct cctcctcccc
    36901 agatgaggcg gacgctcccc ataccccgtg gcggtcaggc gcgcggcggg cggtgtcgcg
    36961 gaagggaccg ggtcgacgtc acctccggcg atagacgccg gacctcttcg accagatctc
    37021 gagcccgggg ccgaggtcgg gcagcgtttc gcccctgccg accaccagcg cgcccccggg
    37081 agccagacgc tccacgatcg tccgcagggc gttgcgctgg cgagcctcgt cgaagtacgt
    37141 gaatgccacg ttccggcaga ggatcaggcc aaacggtccc gccggggccg cctggcggac
    37201 gtcctgctcc acgaatgtca cgccatcccg gtatacctcg cctacgcacc actcttgagc
    37261 cgtcgggacg aaggccagct cgaagtaggc cgccggcagg tccttgaggc tgctccgcgc
    37321 gtagcagccc cggcgagcgc gcgcgagatt ctcgggctcg atgtcggtgg ccagaatgcg
    37381 cagatggaga ccgggcaggc ggaggccagg gcccagacac cagacgacgg cgagcgtata
    37441 gggctcctct ccggccgcgc aaccgatgct ccagcagttg agctcggtct cgcctcggtg
    37501 tacggccagt gctcccagct ccggcagtgt cgaggtagac gcgatatgcg gaaaggcccg
    37561 acaagcccag ctcccggatc cgtcggacca tccgcttgta cacgcgtttc cgcaccttcc
    37621 ggaagcccgg ccagcgcagc cccagccggg gcaggcacca ctggaggaac tggacgccct
    37681 cggagtcctg gatcactggg acggcgctca tgcggtgtcc acgcggacgc ccggtacgcc
    37741 gcttgcaccg ccgtgtcgat catggagggt tccgcgccca ccgaccggca ggctgagcgg
    37801 ttgaccgtct tcctgtgcgg ggacgtcatg acgggccggg gcatcgacca ggcccttccg
    37861 caccctggcc acccgctcat ccacgagccg tccgtcaggg acgccgggca gtatgtgcgg
    37921 ctggccgagg cggcgaacgg cccggtccca cgcccggtcg gcttctcgta catctggggc
    37981 gccgcgctcg acgagctcgc ccgtgtcgcc ccggacgcgc gtgtcatcaa cctggaaacc
    38041 agcatcaccc gcagtgacga atactggccg gagaaggaga tccactaccg gatgcacccg
    38101 gacaacgtcc aatgcctcac cgccgcccag atcgacgtgt gcgtcctggc gaacaaccac
    38161 gtgctggatt tcggccacct cgggctccgg gagacgctgg acacactctc gcaggcccgg
    38221 ttggggaccg cgggagccgg cgacaccttg gcccaagccc gcgcgccggc ggtggtggag
    38281 atctccggaa agggccggct ggtggtcttc ggattcggag acgagtcgag tggcattccc
    38341 tcgagctggg ccgcggcgga cgaccggccg gggatcgacg tcttgccaga tctctcggag
    38401 gccacggcga cggggatcgt ggagcggatc caggcggtga agcgagcgcg ggacgtcgtc
    38461 gtcgcctcca tccactgggg aagcaactgg ggctatgagg tctcccgggg gcagcagcgg
    38521 ttcgcacgcc gtctgctcga tggaggcgtc gacatcgtcc acggtcattc ctcccaccat
    38581 gtccgcccga tcgagctcta ccgggggaag ctcatcctct acggctgcgg cgatttcctc
    38641 aacgactacg aaggcatcgc cggctacgag gagttccggg acgacctgac gctgatgtac
    38701 ttcgcgacac tgcagccgga gaccggccgc ctcgtgagcc tccggatgac gccgatgcac
    38761 atccggaatc tcagagccaa ccgcgccacc ccggaggatg ccgagtggct cgggcacacg
    38821 ctggcccggg tgagccgggc attcggcgcg cgcgtcgagc tcacggccga cggcactctg
    38881 gcgctcctct ccgggtgagg gcgtccgccg ccctcacact cagctcggga gcacgatctc
    38941 gaaccgctcc cacggcatgg ccgtccaggt ctcggtctgc gcgtggccgt acgagcggat
    39001 gatcatcacc accttcgcgg cgatggtctc gtcaggagcc tcgaagatgt ccatgtagtc
    39061 gtacggcccc aggatcgcgt agttggccac ccatttcgcc tgggggcagt cccgtcgaac
    39121 gtgatcggcc accaccttct ccaggcgctt cagctcgccg ggagtcttca cggcctcggg
    39181 ggtaaggcga gtcaacacca cgtaagtcgg catgggattc ctcctttgct ttgggcatca
    39241 aggcataggc ctggtgaagg gcccagggtt cctcctcggt tccgaaagag ggctcggcgc
    39301 tggtcagccg ctcatccact tccggaacgg cctcccggcc ggcgcggagg aaggtgacca
    39361 ccttgcccac gaggacgaag gtattccaga gatacccggc atccagacag acacgggcca
    39421 tcttctcgga tggcttctcc cagaagcggc ggacctccca gacccgccga tcatccgtcg
    39481 ggtccagcgg tctcccgagc tcaatccacc cgtactccac ctcggggctg gtcgcatgag
    39541 ctccgagcag gacgagtcgg tcggggtgct cgtccaccca ggctgcgatc cgcgcgatgt
    39601 acgccatgaa cgtggccggc tccgacacga aatgatcgga gggaaagacc gcgacggtcg
    39661 catcgggatc ccgccgcgcg acccagtgga gggggaagag gatcccggcc gccgtgcccc
    39721 ggtcggccgg ctggaccagc acggacgggg gtgccggccc cgcccagttc ctggcgaaat
    39781 acggggcatg actgcgcagg gtgaccacgg cggcccggac cgaggggatc ccgagcgcca
    39841 cgcggtcgag ggtttgccga agcagagtgc gatctccgag gaccgggacg tactgcttcg
    39901 ggcggtcgtc gccacagatt cgctgggcca gagactgtaa cctcaccccc ccgcccccgg
    39961 cgagcacgac gccccacacg tgattgctca tgtcgggtcc ccctgccgcc gtcgggcacc
    40021 ccgtgattgc tggtgaggaa gctgcgtatc ccggcaagag cgcacgcatc acccgtgcca
    40081 gctcgacttc tccccgcgat ggacgtgata acgctgcggc gagcggcagt cgctctcgtg
    40141 gagatgaggc attggcgcca cacgtgggac acacgtgccg ggccttgccc gtccgtccgc
    40201 acccggctcg ttcgcttggc aacaacttgg cggagggcac ggagcttgct cgcgaaccgg
    40261 gcgtggaagg agcttgagat gaacaaacga gacttcctct cgagagtaca gcaggcagcc
    40321 ggcctccaaa gccccaagga ggccgagcgg cgaagcgtgg cggtcctgac cgccctctcg
    40381 cacgtgctgc cggactcgga ggcgcggcgg cacttcgcct ctcaactgcc cggcttcctc
    40441 aaatcgcggc tcgccgagga gcacacccga ggccttctgc tcacgggcga tgcctttctc
    40501 cagcatctag gccacgcgtt gggcgtccac gcacccgagg cggagcggac gctcacctcc
    40561 gtcttcggcg tcttgaggga cgcggtgtcg cgcggtcagg tcggtgagct cgagacccat
    40621 ctcccgagag agatcgccag tctcctcgag aagaaaacgc ccccgtcccc accttcgtga
    40681 aaggcctcag cgatgatcaa gaagatcaac ggcaagtacg tggtgctttc ggagactact
    40741 ggacggcggt tcggcaccta cgacacgaga gccgaggcgg agcgacgact gcgccagatc
    40801 gagttcttca agcatctgcg aagccgatcc acgcgccctc gcgccgtctc gccgggaggt
    40861 cggtgagatg aacgttcgtg tgcgagtaca gatcgccaag gggaagcttc gcgagtacga
    40921 ggggcggctg aagcagctcg agggccgtct caccgcgcta cgaggtcgag ccgagcagac
    40981 caccggccgg gcccggctcc aactagtccg cgcggagcgg agagtgcgaa ccgcgatgga
    41041 tgccagtgtc gagcgcgtgg agatcgcgat caaggcgctg caggcgcggc tcgagacgac
    41101 gttgagggag acccgggcgt tgtcgcgcgg cgttcagacg ggtatccgca ccggcgcgaa
    41161 gacgtaccgc cggctcggcc gccggtaggc tacccgccca cggctacgga gccttctcgg
    41221 cggtgaccgc gcgcacctgc gcctccagtg agcgctgaag cgagaccagg cgttcgctga
    41281 agctcacggg ataggtccca ccggggcgca gctcgcggag atcctccggc aacgaggcga
    41341 tctgcgcggc cccgtagtca cggatggccg tgagcgccgg gtggggagcg aggaggcggc
    41401 cgcgctccat cacggagcgc agcaggatct cgaccgtgcc cggaggcgcc ggctcctcgt
    41461 gcagagcgac cacatccccc gcgaacccct cgccgcttcg cagccgccga tacacctgct
    41521 tcggggccgg ccacgtttcc ttgccggcgc tgctcttcag taccgggcgg ccgtcgtatt
    41581 cgacgagctt gtacgccatg tcgaggtacg gctggtccgc cgagacgtcc atgcgcgtgc
    41641 cgacaccgaa ggcgtcgatc ggcgcgcccc tagccaggca ctccccgatg ctcgtctcgt
    41701 ccagaccgcc gctcaccacg atcttcacat acgcgagacc ggcctcgtcg aggagtcggc
    41761 ggacctgccg gctcaggccg acgaggtccc cgctgtccag gcgcacgccg gcgagacgct
    41821 ggcccctggt ctccatctcc ttcgccacca ccaccgcctt ccgtgcggcg gcgaccgtgt
    41881 cgtaggtgtc gagcagcagg atggtctgcg cggggaacgc gcgcgcgaag gcgcggaagg
    41941 cgtcgatctc gcggggaaat gccgacacat aggagtgcgc catggtcccg gtcagcggga
    42001 tgccgtagtg gagacccgcg agcacgttgc tggtggtcga gacccccgcg atgaacgcgc
    42061 accgcgccgc cttcatgccc gcatcgatgc cgtgggtgcg ccgcaggccg aactcgctga
    42121 ccggacggcc gcgggccgcg agcacgcacc gcgccgcctt gctcgcgagg agggtctggg
    42181 aatggaccag gttgaggacc gcggtctcca cgagctgcgc ctcgatgatc ggcgcggtga
    42241 tctcgagtag cggctcgtcc tggaagacca cggtgccttc cggcaccgcc cggaccaggc
    42301 cggtgaagcg tagcccgcga aggaactcca gaaaggacgc ctcgaagaga tcgagcgagc
    42361 cgaggtatga ccgggcgtcg tccgagaagc ggaagtcccg caggaagttc aggacgtcct
    42421 cgagtccggc cgcgacgagg aaggagcgcc cgcgggggag atttcgggcg aagagactga
    42481 aggtcgccgg gccgcgcatg ccctcgcgga gatacgaggc ggccatggtg agctcgtaca
    42541 gatcggcgaa gagcgcggcg tcgcgggtca gcagggggcc cgacgcgaac gctccccgcg
    42601 ccgtccctgg acgacgattc ccacgcacgc cgcgctccct cgtcatcgcg actccaccgg
    42661 ggcggactga ccccgggcgg cccgtggatc gtagagcgat gcgagcacgt ccgcatactt
    42721 ctcggcgatc tgcttcctcc ggagcttctg agtcggggtg acctcccccg cctcctcggt
    42781 gagctcgacc ggcagcaccg cgaatcgctt gatccgagcg tacgactgca gatgggtgtt
    42841 cttctcggcg atgatccgct ccacgcggcc tacgacctcg ggatgctgcg acagacgagc
    42901 atggtcggtc cacaggatcc cgcgactccg ggcgagcgcg gccgccgcct cggcgttcac
    42961 cgtcacgaga gcgaccggat agggccggcc atcgccatac accagggcct ggctgacgaa
    43021 cgggtcgctg cgcaggaggc tctcgatggc ctgggggggg atgttgatcc cgcccgacgt
    43081 gacgatgagg tccttcttcc gatcggtgat gaagacaaag ccgtcctcat ccacgcggcc
    43141 gatgtcgccg gtatggagcc agccgtcggc gccgaaggtc tcggcggact cctcgggccg
    43201 cttccagtag ccgcagaggg cgatgttggg cccgcgggcg aggatctccg cgtcgggagc
    43261 cagccggatc tccaccccgg gcaccggctg gccgaccgag ccgaacctga agcgatccgg
    43321 ccgattgaac gtgagcaccg ggcagctctc ggtcaacccg tatccctcga ggatcaggat
    43381 gccggcggcg tgaaagaacg ccgcgatctc ggatggcagc gccgcgccac ccgacaccgc
    43441 gaagcgcagc cggcccccga gggccgcctg caaccgggcg aagaccagcc ggtgggccag
    43501 ccggtggcgg agcgcgagga gggccggcac cggccggccc gcgctccgcc gcccggcgac
    43561 ttcgtggccg acccgcatgg cccaggcgaa aagaccgcgt ctgagcggtg agccccgctc
    43621 gacgtccgcg acgatccggg cgtggacctt ctcgaagacg cggggcaccg ccacgaggaa
    43681 atgggggcgc acctcccgga tatcgtcgcc gagcttgtcg agtgattcgg cgaacgcggt
    43741 ggtgagaccc aggtagaccc ccatgaaggc ctcgaggcgc gcgaacgaat gggcgagcgg
    43801 caggaagagc acgtggacgt cgccggggcg ggcgggcgcc acctgcgtca ccgacgccag
    43861 catggcgacg tggttgtcgt gggtctgcac gactcccttg ggcggccccg tcgtccccga
    43921 ggtgtacacg atggtcgcaa cctgcttcgg gccgacgccc gtgacccgtg cggccagctc
    43981 cgcgccgaac gtggcggcat ggtcgcggcc gagccgccgc agggcgtccc aaccgaggaa
    44041 ggtcgggtcc ggaccctcgt ggccctcgat cacgacgatg cggccaagcc cggccagccg
    44101 cccgcgcacc tccagcacct tggcgagctg agcgtcgtcc tcgacgatca gcacgcgtgc
    44161 ctccgcgtcg ttgacgatgt aggcgacctg ctcggccgtg tagctgggat agatggggac
    44221 ggtgacgccc cccgccgaga agatcgcgaa atcggcctgc acccactcgg cgcgactccg
    44281 cgacaggatg gccaccgggt cgcccgcccg caggcccagc gccaggagcc cgagggccac
    44341 ctcccgcacc tgcgtgccga ccaggctcca gtcgagcgtc tcccagccgc cgctccgctt
    44401 caccatctgg gccggacggt cgccgctccg ctcgacccgg cgccagaaca tccgcgcgag
    44461 cgtatcgacg g
//
LOCUS       DASUFA010000230         3889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_734527, whole genome shotgun sequence.
ACCESSION   DASUFA010000230 DASUFA010000000
VERSION     DASUFA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734527"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /gene="atpD"
                     /locus_tag="VFX87_08715"
     CDS             complement(<1..1123)
                     /gene="atpD"
                     /locus_tag="VFX87_08715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX4995036.1"
                     /translation="MTATATQNIGTILQCIGPVLDVEFEPGHLPEIYNALRVEDATGP
                     TPIRLVAEVQQHIGQNVVRAVAMSSTDGVTRGMQVVDTGGPITVPVGDAPLGRILNVL
                     GEPVDGGAAIPASVERWPIHRETPKFTDLEPKTQIFETGIKVIDLIAPFVKGGKIGLF
                     GGAGVGKTVVIQELINNVQKGHGGRSVFCGVGERTREGNDLYLEFKEAGILDSVALIY
                     GQMNEPPGARLRVGLSGLTVAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPS
                     AVGYQPTLATEMGDLQERITSTNKGSITSVQAIYVPADDLTDPAPATAFAHLDATVVL
                     SRSIAELGIYPAVDPLDSSSRILAPQFVGERHYNVAIGVQ"
     gene            complement(1126..2007)
                     /gene="atpG"
                     /locus_tag="VFX87_08720"
     CDS             complement(1126..2007)
                     /gene="atpG"
                     /locus_tag="VFX87_08720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX4995037.1"
                     /translation="MAKSKALKGRIRSVQNTRKITRTMELVATSKLKRAQDRVVAARP
                     YAAALAEVIADLVTPDLAERFPLLRRPAPPAHGGPVRAAVLLVTSNRGLAGGFNANLI
                     KEARRRIETLEAEGYQVELHSIGRKGAGFFKYLGRPLASQAQDPIEHPSVEAAAALVE
                     GLLADYAAGTLAGVDLVSARFLSPISTPPTTLRVLPVEAPARKAGGAASYILRPGADE
                     IVGQLLPLYVRNQVYRGLVETSAAEHGARRTAMKNATDNAGEILDVLRRTYNRQRQAQ
                     ITQEIAEIVGGAAALQG"
     gene            complement(2008..3585)
                     /gene="atpA"
                     /locus_tag="VFX87_08725"
     CDS             complement(2008..3585)
                     /gene="atpA"
                     /locus_tag="VFX87_08725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX4995038.1"
                     /translation="MATDTILRAGELKDVLLKEIEAADLSAAEAGDVGTVLEVKDGVA
                     RIYGLPKALAGEMLEFTVSETGEVVTGLVLNLEQDSVGAAIMGDYLKLKEGDEVRCTG
                     KVLEVPAGPEMLGRVVDALGRPVDGLGPIQAKGSRLVEMVAPGIIVRQPVKEPLQTGI
                     KAIDAMIPIGRGQRELIIGDRGTGKTAIAIDTIINQKGAGVVCVYVAIGQKRSTVASV
                     VEKLRESGALEYTIVVTASASEPAPLQYIAPYTGCAMAEYFMYEEGRATLCVYDDLSK
                     QAVAYRQMSLVLRRPPGREAYPGDVFYLHSRLLERAAKISEDPVAVKQDARIKKPGGS
                     LTALPIIETQAGDVSAYIPTNVISITDGQIFLTTDLFYANVRPAVDAGISVSRVGGNA
                     QIKAMKQVAGPLRLSLAQFRELEAFAQFGSDLDAATQRQLARGARLVEILKQPQYAPV
                     PVEQQVAIIYVVTNGHLDDVPVEHVRRWEQQFLAYLGASHAPVLEGIRTKKALTDEIT
                     AGLKAAIEGFKPLFRAE"
     gene            3703..>3889
                     /locus_tag="VFX87_08730"
     CDS             3703..>3889
                     /locus_tag="VFX87_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995039.1"
                     /translation="MARLDGITRLAAARASALGDRLLAVLLRLGIGEHALLIGFALLV
                     GTVVGFGTLGFYRAIDVA"
BASE COUNT          511 a         1372 c         1384 g          622 t
ORIGIN      
        1 gctgcacgcc gatcgcgacg ttgtagtgcc gctcgccgac gaactgcggg gcgaggatgc
       61 ggctcgagct gtcgagcggg tccaccgcgg gatagatgcc cagctcggcg atggaccggg
      121 agagcacgac ggtggcatcg aggtgcgcga acgcggtggc cggtgccggg tcggtcaggt
      181 cgtcggcggg cacgtagatg gcctgcaccg acgtgatcga gcccttgttg gtggaggtga
      241 tccgctcctg gaggtcgccc atctcggtgg ccagggtcgg ctggtagccc accgcgctcg
      301 gcatccggcc gagcagcgcc gacacctccg agcccgcctg ggtgaagcgg aagatgttgt
      361 cgatgaagag gagcacgtcc tggccctcga cgtcgcggaa gtactccgcc acggtcaggc
      421 cggagaggcc cacgcggagg cgcgcgccgg gcggctcgtt catctggccg tagatgagcg
      481 ccaccgagtc gaggattccc gcctccttga actcgaggta gaggtcgttg ccctcgcggg
      541 tccgctcgcc cacgccgcag aagaccgacc ggccgccgtg gcccttctgc acgttgttga
      601 tgagctcctg gatgaccacc gtcttgccca cgccggcgcc accgaagagg ccgatcttgc
      661 cgcccttcac gaagggggcg atcaggtcga tgaccttgat gccggtctcg aagatctggg
      721 tcttgggctc gaggtcggtg aacttgggcg tctcccggtg gatcggccac cgctccacgc
      781 tcgccgggat ggcggcaccg ccgtccaccg gttcgccgag gacgttgagg atccgcccga
      841 gcggggcgtc gcctaccggc accgtgatcg ggccgccggt gtcgaccacc tgcatcccgc
      901 gggtgacgcc gtcggtcgag ctcatcgcga cggcgcggac cacgttctgg ccgatgtgct
      961 gctgcacctc ggccacgagg cggatcggcg tcgggccggt cgcgtcctcg acccggaggg
     1021 cgttgtagat ctccggcagg tgcccgggct cgaactcgac gtcgagcacc gggccgatgc
     1081 actggaggat ggttccgatg ttctgcgtgg cggtggcggt catcttcatc cctggagcgc
     1141 agcggcgccg cccacgatct ccgcgatctc ctgcgtgatc tgggcctgcc gctgccggtt
     1201 gtacgtccgg cggagaacgt cgaggatctc cccggcgttg tcggtcgcgt tcttcatggc
     1261 ggtgcgccgg gcgccgtgct cggccgcgct ggtctccacc agcccgcggt acacctggtt
     1321 ccgcacgtag agcggcagca gctgccccac gatctcgtcg gcacccgggc gcaggatgta
     1381 gctggcggcc ccgccggcct tccgggccgg cgcctccacc gggaggaccc ggagggtggt
     1441 cggcggggtg ctgatcgggc tgaggaaccg ggccgagacc aggtcgaccc cggcgagcgt
     1501 gcccgcggcg tagtcggcga ggagcccctc caccagcgcg gcggcggcct ccaccgaggg
     1561 atgctcgatg gggtcctggg cctggctggc gagcgggcgg ccgaggtact tgaagaaccc
     1621 ggcccccttc cggccgatcg agtgcagctc gacctggtag ccctcggcct cgagggtctc
     1681 gatccggcgc cgggcctcct tgatcaggtt cgcgttgaac ccgcccgcga gcccgcggtt
     1741 ggacgtcacc agcagcacgg ccgcgcgcac cggcccgccg tgcgccggcg gcgccgggcg
     1801 gcgcagcagc gggaaccgct ccgccaggtc cggggtcacc aggtcggcga tcacctccgc
     1861 cagcgcggcg gcatagggcc gggcggccac cacccggtcc tgggcccgct tcagcttgga
     1921 cgtggccacc agctccatcg tccgcgtgat cttgcgggtg ttctggaccg agcggatccg
     1981 gcccttcagc gccttcgact tcgccatcta ctcggcccgg aacaggggct tgaagccctc
     2041 gatggcggcc ttgagcccgg cggtgatctc gtcggtcagc gccttcttcg tgcggatccc
     2101 ctcgagcacc ggcgcgtgcg acgcgccgag gtacgcgagg aactgctgct cccagcgccg
     2161 cacgtgctcc accggcacgt cgtcgaggtg tccgttggtg acgacgtaga tgatggccac
     2221 ctgctgctcc accggcaccg gcgcgtactg cggctgcttg aggatctcca ccagccgcgc
     2281 cccgcgggcc agctggcgct gggtggcggc gtcgaggtcg gagccgaact gggcgaacgc
     2341 ctcgagctcg cggaactggg ccagggagag ccggagcggg cccgccacct gcttcatcgc
     2401 cttgatctgc gcgttgccgc ccacccggct caccgagatg ccggcgtcca ccgcggggcg
     2461 cacgttggcg tagaacaggt cggtcgtcag gaagatctgg ccgtcggtga tcgagatgac
     2521 gttcgtcggg atgtaggccg agacgtcgcc cgcctgggtc tcgatgatcg gcagcgccgt
     2581 gagcgagccg cccggcttct tgatgcgggc gtcctgcttc accgccaccg ggtcctcgga
     2641 gatcttcgcc gcgcgctcga gcagccggct gtggaggtag aacacgtcgc cggggtacgc
     2701 ctcgcggccc ggggggcgcc gcagcaccag cgacatctgc cggtacgcca ccgcctgctt
     2761 cgacaggtcg tcgtagacgc agagcgtggc ccggccctcc tcgtacatga agtactccgc
     2821 catcgcgcag ccggtgtagg gcgcgatgta ctggagcggc gccggctccg acgccgaggc
     2881 ggtcacgacg atcgtgtact ccagcgcccc ggactcgcgg agcttctcca ccaccgaggc
     2941 caccgtcgag cgcttctggc cgatggccac gtacacgcag acgacgccgg cgcccttctg
     3001 gttgatgatc gtgtcgatcg cgatggcggt cttgccggtg ccgcggtcgc cgatgatcag
     3061 ctcgcgctgg ccgcggccga tcgggatcat cgcgtcgatc gccttgatgc cggtctggag
     3121 cggctccttc accggctgcc ggacgatgat gccgggggcc accatctcga ccagccgcga
     3181 gcccttggcc tggatcgggc ccagcccgtc caccggccgg ccgagcgcgt ccaccacccg
     3241 cccgagcatc tcggggcccg ccgggacctc gagcaccttg ccggtgcagc ggacctcgtc
     3301 gccctccttc agcttgaggt agtcgcccat gatcgcggcg ccgacgctgt cctgctccag
     3361 gttgagcacg aggccggtca ccacttcgcc ggtctcgctc acggtgaact cgagcatctc
     3421 gccggccagc gccttgggca ggccgtagat ccgggcgaca ccgtccttca cctccaggac
     3481 ggtgccgacg tcccccgcct cggcggcgga gaggtcggcg gcctcgatct ccttgaggag
     3541 tacgtctttg agttcgcccg cgcggaggat agtatcggtg gccattgatc tcggctgttc
     3601 actgaggaaa gtcatggtcc ggctacagct tgtgaaaatt atcacaatac cggggcgcgg
     3661 gcaagcgacg gggcatgaag cccgccgggg gccgcccgcc tagtggcgcg cctcgacggg
     3721 atcacccgcc tggcggcggc gcgcgccagc gccctcggcg accggctgct ggccgtcctc
     3781 ctccgcctgg ggatcgggga gcacgccctc ctcatcgggt tcgcgctcct ggtcgggacg
     3841 gtggtgggct tcgggaccct cggcttctac cgcgccatcg acgtcgccg
//
LOCUS       DASUFA010000231         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1428239, whole genome shotgun sequence.
ACCESSION   DASUFA010000231 DASUFA010000000
VERSION     DASUFA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428239"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFX87_08735"
     CDS             <1..672
                     /locus_tag="VFX87_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX4995040.1"
                     /translation="DPLAAIRDGDARGIPLLISTNLDEARLFAPLDPEATALDEAELL
                     ARCQEAIPGGADAARRAIAVYRDARAARGERVEPGDLWFAIESDRTMRYPAMRLAALQ
                     AAHQPETYAYLFAWPSPAMGGLFGACHALELPFLFGTLEHPLLRPFTGKGPAAHALAE
                     QIQDAWIAFARAGRPGHAGIGDWPAYDATHRRTMILDRSCRVEAAPREPERRFWESLP
                     PGAGA"
     gene            complement(669..1028)
                     /locus_tag="VFX87_08740"
     CDS             complement(669..1028)
                     /locus_tag="VFX87_08740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4995041.1"
                     /translation="MRFRRRGDAIVGSFTPGRAHQGYRGLMSGGLLAAIFDCLHYRLA
                     LVRGVALAVTARIEVDYRAPVPLGRRVRFEARLVARRGRVFDTRAVARLAGGAVAAES
                     RAIYVEIPPSRLPGGGR"
     gene            complement(1135..1917)
                     /locus_tag="VFX87_08745"
     CDS             complement(1135..1917)
                     /locus_tag="VFX87_08745"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4995042.1"
                     /translation="MPSTDNPAAAAQRLDGRVAIVTGASRGLGRAMAVALAQAGADVA
                     VAARARPDLEETARLVGETGRRAIVVETDVTAYSQVEALVRRTIAELGGLHVVVNNSG
                     IARVAPVAQMSPEDFRATLEVNLVGVFNGCRAAAAHLLAQKSGKIINISSVLGHVGLP
                     GYSAYAASKSGVMGFTRALAAEWARHGIQVNAVAPGWFVTEMNADAFADERTRDRLLR
                     DVPLRRTGRLDEIGPLVVYLASAASDFMTGQTLFLDGGHSAV"
     gene            complement(1901..>3433)
                     /locus_tag="VFX87_08750"
     CDS             complement(1901..>3433)
                     /locus_tag="VFX87_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX4995043.1"
                     /translation="PIYPTYPPDLIQYIVNDAGVKTLFVEDPGQLAKVLEVQGKMDGL
                     EQIVVMQGYEGEASPRIMTWDELRRLGRDNVERLKSDLAGRVSEVRPEDIATIVYTSG
                     TTGPPKGVVQTHGNHMGTLESAAKMKTITEGDTHLLFLPLAHSFARLESFLGVHRGLI
                     TAFAENIDKLRDNLPEVKPNFICSVPRVFEKVYAGVIAKAESGSPVKKKIFYWAVGVG
                     REVSRLKQAKKPVPAGLAFKYRLAHKLVFSKLHEALGGRLRFAVSGGAPLSKEIAEFF
                     HAAGILILEGYGLTETCPSLTFNRLDHFKFGSVGQAQPGIEIKIAPDGEILGRGPNIA
                     KGYFKKPEATAEVFLADGWFATGDIGRIDEEGFLFITDRKKDLIVTAGGMNIAPQNIE
                     NLLKGDPFISQVMVHGDKRPYPVAIITLNPEELAKFAKEQGILNTEPAALAKHPKVVE
                     RVARIVEARNAELQSYAKVKKFAILPADFTVENGLLTPTLKVKRRIITDKHRELLDSL
                     YR"
BASE COUNT          479 a         1245 c         1205 g          504 t
ORIGIN      
        1 gatcccctcg ccgccatccg cgacggcgac gcccgcggca tcccgctgct gatcagcacc
       61 aatctggacg aggcccggct cttcgccccg ctggatccgg aggccacggc gctcgacgag
      121 gccgagctgc tggcccggtg ccaggaggcg attcccggcg gggccgacgc ggcacgacgg
      181 gccatcgcgg tctaccgcga cgcgcgggcc gcccgcggcg agcgcgtcga gccgggcgac
      241 ctgtggttcg ccatcgagtc cgaccgcacc atgcggtatc ccgcgatgcg gctcgccgcg
      301 ctgcaggccg ctcatcagcc ggagacctac gcctatctgt tcgcgtggcc ctcccccgcg
      361 atgggcgggc tcttcggcgc ctgtcacgcg ctcgagctgc ccttcctgtt cggcacgctc
      421 gagcacccgc tcctccgccc gttcaccggc aaggggccgg cggcgcatgc cctcgccgag
      481 cagatccagg acgcgtggat cgccttcgcg cgcgccgggc ggccgggcca cgcgggcatc
      541 ggcgactggc ccgcctatga cgcgacccat cgccgcacga tgatcctcga ccgctcgtgc
      601 cgcgtggagg ccgcgccgcg ggaacccgag cgacgattct gggagtcgct cccgcctggg
      661 gccggcgcct agcgcccgcc gccgggcaac cggctcggcg ggatctccac gtagatcgcg
      721 cgagactcgg cggcgaccgc gccgcccgcc aggcgcgcca ccgcgcgcgt atcgaagacg
      781 cggccgcgcc gggcgacgag gcgggcctcg aagcgcacgc ggcgtccgag cggaaccggc
      841 gcgcgatagt ccacctcgat ccgcgcggtc accgccaggg ccacgccccg cacgagcgcc
      901 aggcgatagt gcaggcagtc gaagatcgcg gcgagcaggc cccccgacat gaggccgcgg
      961 tagccctgat gggcgcggcc cggcgtgaac gagccgacga tcgcatcgcc ccgtcgccgg
     1021 aaccgcacgc gcaggccggt gcggctcgca ggcccgcaaa tgacgcactc cgcgagcggc
     1081 ggcgggtgga gcgcgagccg cgcgggcgat cgggtcgcgc ggcggcgccc ggcctcagac
     1141 cgccgagtgg ccgccatcga ggaagagggt ctggccggtc atgaagtcgg aggcggcgga
     1201 ggccaggtag acgacgagcg ggccgatctc gtcgagccgg ccggtgcgcc ggagcggcac
     1261 gtcgcggagc agccggtcgc gcgtgcgctc gtccgcgaac gcgtccgcgt tcatctcggt
     1321 gacgaaccag cccggcgcga ccgcattgac ctggatgccg tggcgcgccc actccgcggc
     1381 cagcgcgcgg gtgaacccca tgaccccgct cttggaggct gcgtacgcgc tgtagccggg
     1441 gaggcccacg tgccccagca cggaagagat gttgatgatc ttccccgact tctgggccag
     1501 gagatgcgcg gcggcggccc ggcagccgtt gaagacgcca accagattga cctccagcgt
     1561 ggcgcgaaag tcctcggggg acatctgggc gaccggggcc actcgcgcga tgccggagtt
     1621 gttcaccacc acgtgcaggc cgcccagctc cgcgatggtg cggcgcacga gcgcctccac
     1681 ctgcgagtag gcggtgacgt ccgtctccac cacgatggcc cgccggccgg tctcgccgac
     1741 cagccgcgcc gtctcctcca gatcgggccg ggcccgcgcg gccaccgcga cgtccgcgcc
     1801 ggcctgcgcc agcgccaccg ccatcgcgcg cccgaggccg cgactggccc cggtgacgat
     1861 ggccacgcgg ccatcgaggc gctgggcggc cgcggccggg ttatcggtag agggaatcga
     1921 ggagctcccg gtgcttgtcg gtgatgatcc gtcgcttgac cttcagcgtg ggcgtcagca
     1981 ggccgttctc caccgtgaag tcggcgggca ggatcgcgaa cttcttgacc ttggcatagg
     2041 actgcagctc cgcgttgcgc gcctccacga tccgggccac gcgctcgacc accttcgggt
     2101 gcttggcgag ggccgcgggc tccgtgttga ggatcccctg ctccttggcg aacttcgcca
     2161 gctcctccgg gttcagcgtg atgatggcca ccgggtatgg gcgcttgtcg ccgtgcacca
     2221 tcacctggct gatgaacgga tcgcccttga gcaggttctc gatgttctgc ggcgcgatgt
     2281 tcatcccgcc cgcggtcacg atgaggtcct tcttccggtc ggtgatgaag aggaagccct
     2341 cctcgtcgat ccgcccgatg tcgccggtgg cgaaccagcc gtcggccagg aacacctcgg
     2401 cggtggcctc cggcttcttg aagtagccct tggcgatgtt gggtccgcgg cccaggatct
     2461 cgccgtcggg cgcgatcttg atctcgatgc cgggctgcgc ctgccccacc gacccgaact
     2521 tgaagtggtc gagccggttg aaggtgagcg aggggcaggt ctcggtcaga ccgtagccct
     2581 cgaggatcag gatgcccgcc gcgtggaaga actccgcgat ctccttggag agcggcgcgc
     2641 cccccgagac cgcgaagcgc aggcgcccgc cgagggcctc gtggagcttc gagaacacga
     2701 gcttgtgggc caggcggtac ttgaaggcga gcccggccgg caccggcttc ttggcctgct
     2761 tcaagcgcga gacctcccgg cccaccccga ccgcccagta aaagatcttt ttcttgaccg
     2821 gcgagccgga ctccgccttg gcgatcacgc ccgcgtagac cttctcgaac acgcgcggca
     2881 cgctgcagat gaaattgggc ttcacctcgg gcaggttgtc ccgcagcttg tcgatgttct
     2941 cggcgaaggc ggtgatgagc ccccggtgca cgcccaggaa cgactcgagc cgcgcgaacg
     3001 agtgggccag cggcaggaac agcaggtggg tgtccccctc ggtgatggtc ttcatcttgg
     3061 ccgccgactc cagggtgccc atgtggttgc cgtgcgtctg caccacgccc ttgggcggcc
     3121 cggtggtccc ggaggtgtag acgatggtcg cgatgtcctc ggggcggacc tcgctcacgc
     3181 gcccggcgag gtcgctcttg agacgctcca cgttgtcgcg ccccagccgg cgcagctcgt
     3241 cccacgtcat gatccgcggc gaggcctcgc cctcgtaacc ctgcatcacc acgatctgct
     3301 cgagcccgtc catcttcccc tgcacctcga gcaccttggc gagctggccg gggtcctcga
     3361 cgaagagggt cttcacgccc gcgtcgttga cgatgtactg gatcaggtcg ggcggatagg
     3421 tcgggtagat cgg
//
LOCUS       DASUFA010000232         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1428292, whole genome shotgun sequence.
ACCESSION   DASUFA010000232 DASUFA010000000
VERSION     DASUFA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428292"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VFX87_08755"
     CDS             complement(<1..1201)
                     /locus_tag="VFX87_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995044.1"
                     /translation="MALGGVVLALLVLQGGSDTLSYANPATRALVERAAERHRVQESE
                     VSDYRARLRFRTSFSLGRRAWARLPVASAEELDATVHWSAPNDLRVDMHGRRGKSRLE
                     DADIRTVFSRPWFFPRGVGDSVRLFGEDFPEQAALHPLAKDGPEWYRYAIVDSQRVVR
                     PDGEPIRLYTVEVAPRRTGPALVAGFLLLDAGRAEVVRFSFRFVGTALWDVPTEGTPS
                     DSAESRRANRLVNRILQLDADLEYALQEGRFWMPYRQVISGRVQVPFIGDIVIPFEMR
                     TLFDDYEVNTGQAVAFSVPIPPEGMSKEERRAWRDSVREARGEERADSTRSWDAAGRW
                     QGGSYQVHHAPEDSMRAWGGWTDSLVLDPGGAEEAELRDVFGDLARLSEDLPGDMVGR
                     PTWAYGYE"
     gene            complement(1183..1641)
                     /locus_tag="VFX87_08760"
     CDS             complement(1183..1641)
                     /locus_tag="VFX87_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4995045.1"
                     /translation="MTETQPAPSLPSVFSPLARALLDGFAEGVVVFDPQGKVLYANQP
                     AREALTGQVDLSQPAGILGPQLAEIGGRVRPLRIGSLDLGEAMFIPGPASATTLAARE
                     KEAIVASLEAHNWRLAETAKHLGISRTTLWRRLKAYGLHRDGRSRWPSEE"
     gene            complement(1705..2622)
                     /locus_tag="VFX87_08765"
     CDS             complement(1705..2622)
                     /locus_tag="VFX87_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4995046.1"
                     /translation="MNALVHLALILVQALFASLAITGKVVLREFPPMGLVLVRVGGAA
                     LLLGLGNLLWRGPWIRDRADLGRLAFLALLGVVANQGLFLIGLRHTTATNATIIVATI
                     PVFTVLYSVLGRREPPSFLKLAGIALAAAGSVWLIGPDRIALEREKALGNAIILAGMA
                     CYGWYLVQSKELLRRYEPITVSVYVMAFAALFTLPFGAWAIARADLAAVRPVTWAWIG
                     YIVLFPTIVTYFLNIWALSRVSSNLVAAYIYLQPVFTAAVAPMVLEGEALTGRAIGAG
                     LVIFAGLACVILGERRQKGQVPVGAVVGE"
     gene            complement(2647..>3149)
                     /locus_tag="VFX87_08770"
     CDS             complement(2647..>3149)
                     /locus_tag="VFX87_08770"
                     /inference="COORDINATES: protein motif:HMM:NF039197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HEX4995047.1"
                     /translation="RSEAYQVGFRLAKRLGHARVYAIDYKQDMRVGEVLAWGPEHGQG
                     ATVGQIQGFMGKAMPLMQRMLGEMTMGQIYARASRPDFLSLTHEPYLLMARIGDGTEE
                     PGWQDVSGWYARNMRIFVNLTRIVSAPDDRVLVIFGQGHAPLLRHYVGASGDFTLAEP
                     ADYIRE"
BASE COUNT          480 a         1133 c         1128 g          408 t
ORIGIN      
        1 gctcgtagcc gtaggcccac gtgggccggc ccaccatgtc cccggggagg tcctccgaga
       61 gccgggcgag gtcgccgaag acgtcccgca gctcggcctc ctcggccccg ccgggatcga
      121 gcaccagcga gtcggtccag ccgccccacg cgcgcatcga gtcctccggc gcgtggtgca
      181 cctggtagct gccgccctgc caacgcccgg cggcgtccca ggagcgggtc gagtccgccc
      241 gctcctcccc ccgggcctcg cgcaccgagt cgcgccaggc gcgccgctcc tccttcgaca
      301 tcccttccgg cgggatcggc accgagaagg ccaccgcctg cccggtgttc acctcgtagt
      361 cgtcgaacag ggtgcgcatc tcgaacggga tgacgatgtc cccgatgaag gggacctgca
      421 cccgccccga gatcacctgg cggtacggca tccagaagcg gccctcctgc agcgcgtatt
      481 cgaggtcggc gtcgagctgg aggatccggt tcaccagccg gttggcccgg cggctctcgg
      541 cggagtcgga gggcgtccct tcggtcggga cgtcccacag cgcggtgccg acgaagcgga
      601 aggagaagcg caccacctcg gcgcgcccgg cgtcgagcag gaggaagccc gccaccagcg
      661 ccgggccggt ccgccgcggc gccacctcga ccgtgtacag ccggatcggc tcgccgtcgg
      721 gccgcaccac gcgctggctg tccacgatcg cgtagcggta ccactcgggg ccgtccttcg
      781 cgagcggatg gagggcggcc tgttccggga agtcctcgcc gaagagccgc accgagtcgc
      841 ccaccccgcg ggggaagaac cagggccgcg agaacacggt gcggatgtcg gcgtcctcga
      901 gccgcgactt gccgcgccgg ccgtgcatgt ccacccggag gtcgttcggc gccgaccagt
      961 gcaccgtcgc gtcgagctcc tcggccgacg ccaccggcag gcgggcccac gcccgacgcc
     1021 cgagggagaa ggaggtccgg aaacgaagcc gggcccggta gtcggaaact tccgactcct
     1081 ggacccggtg ccgttccgcg gcgcgctcga cgagggcgcg cgtggcggga ttcgcgtagc
     1141 tcagcgtgtc gctgccgccc tggaggacga gcagcgcgag gactactcct ccgagggcca
     1201 ccggctccgc ccatcccggt ggagcccgta tgccttgagc cgacgccaga gggtggtgcg
     1261 gctgatcccg aggtgcttgg cggtctccgc caggcgccag ttgtgcgcct cgagcgaggc
     1321 gacgatcgcc tccttctcgc gggcggccag cgtggtggca ctggccggtc cggggatgaa
     1381 catcgcctcc ccgaggtcga gcgagccgat ccggagcggg cgcacccggc cgccgatctc
     1441 ggcgagctgg gggccgagga tgccggccgg ctgggacagg tccacctggc cggtcagcgc
     1501 ctcgcgcgcg ggctggttgg cgtagagcac cttcccctgg gggtcgaaca ccaccacccc
     1561 ctccgcgaac ccgtccagca gggcccgggc gaggggcgag aagaccgagg gaagtgaggg
     1621 agcgggctgg gtttccgtca tcggcatatg ggccttacgc tggggcagtc accgaggttt
     1681 caaggaggga cgttaacctg gtggctactc tcccaccacc gcccccaccg gcacctgccc
     1741 cttttgacgc cgctccccga ggatcacgca cgccagcccg gcgaagatga ccagccccgc
     1801 cccgatggcc cggccggtga gggcctcccc ctccagcacc atcggcgcca ctgccgcggt
     1861 gaacaccggc tggaggtaga tgtacgccgc cacgaggttg gacgagaccc gggacagcgc
     1921 ccagatgttc aggaagtagg tgacgatcgt cgggaagagg acgatgtagc cgatccaggc
     1981 ccaggtgacc ggccggaccg ccgcgaggtc ggcccgggcg atcgcccagg cgccgaaggg
     2041 cagggtgaag agtgcggcga acgccatcac gtacacgctc acggtgatcg gctcgtaccg
     2101 ccgcagcagt tccttcgact ggaccaggta ccagccgtaa caggccatcc ccgccaggat
     2161 gatggcgttg cccagcgcct tctcgcgctc gagcgcgatc cggtcggggc cgatgagcca
     2221 gaccgacccg gcggcggcga gtgcgatgcc cgcgagcttc aggaaggacg gcggctcccg
     2281 gcggccgagc accgagtaga gcaccgtgaa gacggggatg gtcgccacga tgatcgtggc
     2341 gttcgtggcc gtggtgtgcc ggagcccgat gaggaagagc ccctggttcg ccaccacgcc
     2401 gaggagcgcc aggaaggcga gccgcccgag gtcggcgcgg tcccggatcc agggcccgcg
     2461 ccagagcagg ttgccgaggc cgagcagcag ggccgccccg cccacccgca cgagcacgag
     2521 ccccatcggc gggaactccc gcagcaccac cttccccgtg atggccaggc tggcgaagag
     2581 cgcctggacg aggatcagcg cgaggtggac gagggcgttc atcggcgcgg caatctagcc
     2641 gcgcggtcac tcccggatgt agtcggccgg ctcggcgagg gtgaagtcac cggaagcccc
     2701 cacgtagtgc cggaggagcg gggcgtggcc ctggccgaag atcacgagca cccggtcgtc
     2761 cggcgcggac acgatgcggg tcaggttgac gaagatccgc atgttccggg cgtaccaccc
     2821 gctcacgtcc tgccagccgg gctcctcggt gccgtcgccg atgcgcgcca tcaggaggta
     2881 gggctcgtgg gtgagcgaga ggaagtcggg ccggctcgcg cgggcataga tctgtcccat
     2941 ggtcatctcg cccagcatcc gctgcatcag cggcatcgcc ttccccatga agccctggat
     3001 ctggccgacg gtggcgccct gcccgtgctc cgggccccac gcgagcactt caccgacgcg
     3061 catgtcctgc ttgtagtcga tcgcgtagac gcgcgcgtgc ccgagccgct tcgccaggcg
     3121 gaagccgacc tggtaggcct cgctccggc
//
LOCUS       DASUFA010000233         6374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_630004, whole genome shotgun sequence.
ACCESSION   DASUFA010000233 DASUFA010000000
VERSION     DASUFA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630004"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFX87_08775"
     CDS             <1..747
                     /locus_tag="VFX87_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4995048.1"
                     /translation="RCHYCRRGSPSMCRHFKRVNLDPGGFAELVRVPAPNVEHAAFRL
                     PAEMAEETASFTEPLACCLRAVKRSPVAVGDTALVVGLGSIGCLLAQLLALEGARVLA
                     SDLLEDRRALGRRLGAQVVDTDAALDAALREATEGRGADLVIVTAGGPAVLPAAAARV
                     RDGGTLHYFAGGAGDSLPLSLGALYHRELTLTATYSSSPTELAEAFALLAGDRIRVDG
                     LISHRLPLGRLGEGVELMRRHEAVKVYVIP"
     gene            750..1781
                     /locus_tag="VFX87_08780"
     CDS             750..1781
                     /locus_tag="VFX87_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4995049.1"
                     /translation="MRAQVFHGPGDLRFEDLPVPEPEPGGLVVRVEAALTCGTDVKTL
                     RRGHPVMIPHVPTVFGHELAGVVTAVGRGAPGVKEGDRVVAANSAPCGRCRLCAAGRP
                     NLCEDLLFVNGAYGQYIALPPRLAARNVVPIGPGLAAPIAAFAEPLACALLGVERGRV
                     EAGMSVVVWGHGPLGCLLALAARQRGAQVVIVGKAGWRLDRVRELGLGTCVDALSVPD
                     MGAHLRELTGGRGPDVTVDATGRPEVWEQAIDCVGRGGTVILFGGCAPGTSVRVDTRR
                     AHYEELTLVGAFHHTPAMIRRAVELLESKAIEPAGLLTHRMGLGQVKEALALMGRGET
                     MKVLVDPWQ"
     gene            1778..2395
                     /locus_tag="VFX87_08785"
     CDS             1778..2395
                     /locus_tag="VFX87_08785"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX4995050.1"
                     /translation="MSTSKNRSRWAMAATVTLAVLALVRPAPAYDAEATFARGTTIFG
                     LQVGGGAANNVEGHRTVSDISFLTATPRVSHLFFSPFGSGLLRSAVEPGIEGWFQYYL
                     SPNEATAQGIKLGMRYHLIGLGRLVPYVEAITGVGGTSLRVMEINSTFTFVLEAGAGL
                     SYFIMDTVALNAGYRFQHISNGHTSNPNRGFNSDTGIGGVSFYFK"
     gene            2403..3407
                     /gene="ispH"
                     /locus_tag="VFX87_08790"
                     /gene_synonym="lytB"
     CDS             2403..3407
                     /gene="ispH"
                     /locus_tag="VFX87_08790"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX4995051.1"
                     /translation="MTTPLPLKQIVLAGPRGFCAGVDRAIDIVELALQVCPPPVYVRK
                     EIVHNRHVVEALRAKGAHFVDELDEVPDDATVIFSAHGISPAVRAEAHRRGLRVIDAT
                     CPLVTKVHLEAVRYAREGYSIILVGHADHDEVVGTMGEAPDRMFLIAHPEDVDKLDVP
                     DPDKVAYLTQTTLSVDDTRDCIEALRRRFPTIVGPAKDDICYATQNRQAAVKMVAGAV
                     DVLLVIGAANSSNANRLVEVSTVMGTPSYLINDKNDIRPEWLEGMGRVGVTAGASTPE
                     HLVTEVVDELRRVHRSEVREVHVVDEDVRFGLPRELEDIAKAAGKPLPPRSGAVAVSG
                     "
     gene            3411..4352
                     /locus_tag="VFX87_08795"
     CDS             3411..4352
                     /locus_tag="VFX87_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4995052.1"
                     /translation="MRLVTFRRGRGEPKPGALWQEHVLDLTGLAKELATQRGTARRGR
                     GGFPRTLLEIVQGGEAAMALTREAWEYGQRLVGQTAAEELAQRRLAYPAKRVRLLAPI
                     PSPARNVFCLGRNYAEHAAERGAAAPEHPVYFTKPATAVVGPGDDVVHHAITKELDYE
                     VELTAVIGTGGRDIPRADALKHVFGYTIINDVTARDLQKRHQQWFKGKSLDTFCPMGP
                     VLVTVDEVPDPQALAISLKVNGQLRQSSHTSKMIFPVAQCIEVLSQGMTLLPGDIIAT
                     GTPEGVGAASGNFLKAGDRIEAEVERIGILHSKIVRP"
     gene            4581..6038
                     /locus_tag="VFX87_08800"
     CDS             4581..6038
                     /locus_tag="VFX87_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995053.1"
                     /translation="MRTVIEGGWVVAWNGKSHEVHEQGSVVFEGDRVVHAGPPWTGAA
                     DVRIAARGKLVSPGFINTHVHTAGGGGDYLLLDMAKNDYRTANYMAFAAPLKGKMTPP
                     PAEPTAAIRAFTFLHALKNGTTTVIDVGGLRGDWEGYARLVDELGVRVYGGPPFRDRD
                     TYTDAQGRLYYDEDAAVGRSRLQEAVDFGRKFDGAAGGRLRVLFNGAQVETCSEPLLR
                     AGKDAARAQNAPIHTHAGGNLVEFQRIMDEYRKTPIRFLADIGFLDDRTLIGHAVFTT
                     AHDWTRYPFGDDLRVLAERGSTVGHCPYKYAKMAMTLRSFQRYLDAGVTLALGTDTYP
                     LDMVSELRWASILAKVTDANYQAGLARDVFNAATVGGCRFLGREDLGRLAPGAKADIL
                     LINLDHVGGSLYADPIKALVDSASGRDVDTAIVDGQVLVQGGRATRVDEDAVYAKARE
                     ATQHYWSRVPGWRWDSATADQIVPPAFPIHRAARA"
     gene            6127..>6374
                     /locus_tag="VFX87_08805"
     CDS             6127..>6374
                     /locus_tag="VFX87_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995054.1"
                     /translation="MSPTGTMTKRQRVTAALAGQAVDRVPLAFWLHNFATENSAKGLA
                     DETLRLAHTFDWDFLKPQSRAQCFAEAWGLEYRASSARA"
BASE COUNT          894 a         2236 c         2336 g          908 t
ORIGIN      
        1 cgctgccact actgccggcg cggcagcccg tccatgtgcc gccacttcaa gcgggtcaat
       61 ctcgatccgg gcggattcgc ggagctggtg cgcgtgcccg cgcccaacgt ggagcacgcc
      121 gccttccggc ttcccgccga gatggccgag gagaccgcgt ccttcaccga gccgctggcc
      181 tgctgcctgc gcgcggtgaa gcggagcccg gtggccgtgg gtgacaccgc gctcgtggtg
      241 ggcctgggct cgatcggctg ccttctggcg cagctcctgg ccctcgaggg cgcgcgcgtc
      301 ctggcctccg atctgctgga ggaccggcgc gcgctggggc gccgcctggg tgcacaggtg
      361 gtcgataccg acgccgcgct ggacgcggcg ctgcgcgagg ccaccgaggg gcgcggggcc
      421 gacctcgtga tcgtcaccgc cggcggcccc gccgtgctgc ccgccgcggc cgcgcgcgtg
      481 cgggacgggg ggacgctgca ctacttcgcc ggcggcgcgg gcgacagcct gcccctgtcg
      541 ctcggggcgc tctaccatcg cgagctgacc ctcaccgcga cctattcgtc ctcgcccacc
      601 gagctcgcgg aggccttcgc gctgctggcc ggggaccgga tccgggtgga cggcctcatc
      661 agccaccggc tcccgctcgg gcgcctgggc gagggggtgg agctgatgcg ccgccacgag
      721 gcggtgaagg tctacgtgat cccgtagcca tgcgggccca ggtgttccac ggtccggggg
      781 atctgcgctt cgaggacctc ccggtgcccg agccggagcc gggcggcctc gtggtgcggg
      841 tggaggcggc gctcacctgc ggcaccgacg tcaagaccct gcgccgtggt catcccgtga
      901 tgatcccgca cgtgcccacc gtgttcggcc acgagctggc cggcgtggtc accgcggtgg
      961 ggcggggcgc tcccggggtg aaggagggcg accgggtcgt cgccgccaac tcggcgccgt
     1021 gcgggcggtg ccgactctgc gcggccggcc ggccgaacct ctgcgaggac ctgctcttcg
     1081 tcaacggggc ctacggtcag tacatcgcgc tgcccccgcg tctggcggcc cggaacgtgg
     1141 tgccgatcgg gcccgggctg gccgccccga tcgccgcctt cgccgagccg cttgcctgtg
     1201 ccctgctggg ggtcgagcgg ggccgcgtgg aggccggcat gtccgtggtg gtctggggcc
     1261 acggcccgct gggctgcctg ctggccctgg ccgcccggca gcgcggggcc caggtggtca
     1321 tcgtgggcaa ggcgggctgg cgcctggacc gagtccgcga gctggggctg gggacctgcg
     1381 tggatgccct ctccgtcccg gacatggggg ctcacctgcg ggagctgacc ggggggcggg
     1441 ggcccgacgt caccgtggac gcgaccggcc ggcccgaggt ctgggagcag gccatagatt
     1501 gcgtgggccg gggaggcaca gtgatattgt ttggtggctg cgcgccgggc acctcggttc
     1561 gggtggacac gcgtcgggcg cattacgaag agctcacgct ggtgggggcc ttccaccaca
     1621 ccccggccat gatccgacgc gcggtggaac tccttgaatc gaaggcgatc gagcctgcgg
     1681 ggcttctgac tcatcgcatg gggctggggc aggtaaaaga agcgctggcc ctgatggggc
     1741 gtggtgagac catgaaggtc ctggtggatc cctggcaatg agcacgagca agaaccggag
     1801 tcgatgggcg atggcggcca cggtcacgct ggccgtgctg gccttggtgc gcccggcgcc
     1861 cgcctatgac gcggaagcca cgttcgcccg ggggaccacg atcttcggcc tgcaggtcgg
     1921 gggcggcgcg gcgaacaacg tagagggcca ccggacggtc agcgacatct cgttcctgac
     1981 cgcgaccccg cgggtgtccc acctgttctt ttcccccttc gggagcgggc tcctccggag
     2041 cgcggtcgag ccgggcatcg aaggctggtt ccagtactac ctctcgccga acgaggcaac
     2101 cgcccagggc atcaagctcg ggatgcggta tcatctgatc ggcctgggcc ggctggttcc
     2161 gtatgtcgag gccatcacgg gggtgggggg tacgagtctc cgggtgatgg agattaactc
     2221 gaccttcacc ttcgtgctcg aggccggggc ggggttgtcg tacttcatca tggacacggt
     2281 cgcgctgaat gcgggctatc gcttccagca catctcgaac gggcacacct cgaacccgaa
     2341 ccggggtttc aactcggaca cggggatagg cggcgtttcg ttctatttca agtgagggca
     2401 agatgaccac gccgctgccg ctcaaacaga tcgtgctggc cggaccccgg ggcttctgcg
     2461 ccggggtgga ccgcgccatc gacatcgtcg agctcgcgct ccaggtgtgc ccgcctcccg
     2521 tgtacgtgcg caaggagatc gtgcacaacc gtcacgtggt cgaggcgctc cgggccaagg
     2581 gtgcccactt cgtggacgag ctggacgagg tgccggacga cgccaccgtg atcttcagcg
     2641 cccacggcat ctcgccggcg gtgcgcgcgg aggcccaccg gcgcgggctg cgggtgatcg
     2701 acgccacgtg cccactcgtc accaaggtgc atctggaagc cgtccgctac gcgcgcgagg
     2761 ggtactccat catcctggtc ggccacgccg atcacgacga ggtggtgggc accatgggcg
     2821 aggcgccgga ccgcatgttc ctgatcgccc acccggagga cgtggacaag ctcgacgtcc
     2881 cggaccccga caaggtggcg tatctcaccc agaccacgct gtcggtggac gacacccgag
     2941 actgcatcga ggcgctccgg cgccgcttcc ccacgatcgt cgggccggcc aaggacgaca
     3001 tctgctacgc gacccagaac cgccaggcgg cggtgaagat ggtggcgggc gcggtggacg
     3061 tgctgctggt catcggcgcg gccaacagct cgaacgccaa ccgcctcgtc gaggtctcca
     3121 ccgtcatggg cacgccgtcc tacctcatca acgacaagaa cgacatccgc ccggagtggc
     3181 tcgagggcat ggggcgcgtc ggggtcaccg cgggcgcctc cacgcccgag cacctcgtga
     3241 cggaagtggt ggatgagctg cggcgcgttc accgctcgga ggtcagagag gtccacgtgg
     3301 tggacgagga cgtgcgcttc ggcctgccgc gggagctgga ggacatcgcc aaggccgcgg
     3361 gcaagccgct gccgccgcgc agcggcgcgg tggcggtgag cggctaggcc atgcggctcg
     3421 tcaccttccg gcgcgggcgc ggcgagccca agcccggcgc gctctggcag gagcacgtgc
     3481 tcgacctgac cggtctcgcc aaggagctcg cgacgcagcg gggcaccgcg cgccgggggc
     3541 gcggcggctt cccgcgcacg ctgctcgaga tcgtgcaggg cggcgaggcg gcgatggcgc
     3601 tcacccgcga ggcgtgggag tacggccagc ggctcgtggg gcaaaccgcc gcggaggagc
     3661 tggcccagcg gcggctggcc tacccggcca agcgggtgcg gctgctcgcc ccgatcccgt
     3721 cgccggcccg taacgtcttc tgcctcggcc gcaactacgc cgagcacgcc gccgagcggg
     3781 gcgccgccgc tcccgagcat ccggtgtact tcaccaagcc cgcgaccgcg gtggtggggc
     3841 cgggcgacga cgtggtgcac cacgcgatca ccaaggagct cgactacgag gtcgagctga
     3901 ccgcggtgat cggcaccggc gggcgcgaca tcccgcgcgc cgacgcgctc aagcacgtct
     3961 tcggctacac catcatcaac gacgtgaccg cgcgggatct gcagaagcgc caccagcagt
     4021 ggttcaaggg caagtccctc gacaccttct gcccgatggg cccggtgctg gtcaccgtcg
     4081 acgaggtgcc ggacccgcag gccctcgcga tctccttgaa ggtgaatggt cagctgcggc
     4141 agtcgagcca cacctccaag atgatcttcc cggtggccca gtgcatcgag gtgctctcgc
     4201 agggcatgac gctcctgccc ggggacatca tcgccaccgg gacccccgag ggcgtcggcg
     4261 cggccagcgg caacttcctc aaggcgggcg accgcatcga ggccgaggtc gagcgcatcg
     4321 gaatactcca cagtaagatc gttcgccctt aggcgggccg cctgggcgga gcgccgataa
     4381 gggcccatct gcttcgttgg cgccctcgtc cgggcgctca acgtacaggg agtacgcctc
     4441 gcgcccggcc tccgggcgcc gcctcgcagc tggacccttc tcgacgcccc ggtaacgctc
     4501 tcgaagcact accttccctg ggcctgattg cgggtttgcc cttggcgggc tagcatgctg
     4561 cccctggagg gggtgcggcc atgcgaacgg tgatcgaggg cgggtgggtc gtggcgtgga
     4621 atggcaagag ccacgaggta cacgagcagg gcagcgtggt gttcgagggc gaccgggtcg
     4681 tgcacgcggg gcccccgtgg acgggtgcgg cggacgtgcg catcgcggcg cgcggcaagc
     4741 tggtctcgcc cggcttcatc aacacccacg tgcacaccgc gggcggcggc ggcgactacc
     4801 tgctgctcga catggccaag aacgactacc gcaccgcgaa ctacatggcc ttcgcggcgc
     4861 cgctcaaggg caagatgacg ccgccccccg ccgagcccac cgcggcgatc cgcgccttca
     4921 cgttcctgca cgcgctgaag aacgggacga ccaccgtcat cgacgtgggc ggcctgcgcg
     4981 gcgactggga gggctacgcg cgcctggtgg acgagctcgg ggtgcgcgtc tacgggggcc
     5041 cgccgttccg cgatcgcgac acctacacgg acgcgcaggg ccgcctctac tacgacgagg
     5101 acgcggcggt cggccggtcg cgcctgcagg aggcggtgga cttcgggcgc aagttcgacg
     5161 gcgcggccgg gggccgcctg cgcgtcctct tcaacggggc ccaggtcgag acgtgcagcg
     5221 agccgctgct gcgggccggc aaggacgcgg cgcgcgcgca gaacgcgccg atccacaccc
     5281 acgcgggcgg caacctcgtc gagttccagc gcatcatgga cgagtaccgc aagacgccga
     5341 tccgcttcct ggccgacatc ggcttcctcg acgaccgcac gctgatcggg cacgcggtct
     5401 tcaccaccgc gcacgactgg acgcgctacc cgttcggcga cgacctgcgc gtgctggccg
     5461 agcgcggctc gaccgtcggc cactgcccgt acaagtacgc gaagatggcg atgacgctcc
     5521 gctccttcca gcgctacctg gacgcgggcg tgaccctggc cctcggcacc gacacctatc
     5581 cgctcgacat ggtgtcggag ctccggtggg cgtccatcct ggccaaggtc accgacgcga
     5641 actatcaggc cggcctcgcc cgcgacgtgt tcaacgcggc caccgtcggc ggctgccgct
     5701 tcctgggacg cgaggatctg gggcgtctgg ccccgggcgc caaggccgac atcctgctga
     5761 tcaacctgga ccacgtcggc gggtcgctct acgcggatcc gatcaaggcg ctggtggact
     5821 cggcctcggg ccgcgacgtg gataccgcca tcgtggacgg ccaggtgctg gtgcagggcg
     5881 gccgggccac gcgcgtggac gaggacgcgg tgtacgcgaa ggcgcgcgag gccacccagc
     5941 actactggag ccgcgtgccg ggctggcggt gggacagcgc caccgcggat cagatcgtcc
     6001 cgcccgcgtt cccgatccac cgggcggcgc gcgcgtgagg cgatcggccc ggctcgcgct
     6061 gcggctccgg ctcggggtct gcgcccacgc ggcgcccgcc gcggccgcgc tgaggatccg
     6121 cgcgcgatga gccccaccgg cacgatgacc aagcggcagc gggtgacggc ggcgctcgcc
     6181 ggccaggcgg tggaccgggt gccgctcgcg ttctggctcc acaacttcgc gaccgagaac
     6241 tcggcgaagg ggctggccga cgagacgctg cgtctcgcgc acaccttcga ctgggacttc
     6301 ctcaagccgc agtcgcgggc ccagtgcttt gccgaggcgt ggggactcga gtaccgcgcc
     6361 tcgagcgccc gcgc
//
LOCUS       DASUFA010000234         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1298732, whole genome shotgun sequence.
ACCESSION   DASUFA010000234 DASUFA010000000
VERSION     DASUFA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298732"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFX87_08810"
     CDS             complement(<1..183)
                     /locus_tag="VFX87_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995055.1"
                     /translation="MGYVERHLLPGERVLYKTRLHWVLFLKAWLLLLAGVALIVLLRL
                     VQDPPWIWMIGAAVALI"
     gene            complement(227..760)
                     /locus_tag="VFX87_08815"
     CDS             complement(227..760)
                     /locus_tag="VFX87_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4995056.1"
                     /translation="MERYVIREVPGRKPRVHPEAYVDPAAQVIGDVTIEAGASVWPGT
                     VLRGDQDNYVKLGRNSNVQDNSVLHVTPQFPCIVGDNVTIGHRAVVHACTVMNNSRIG
                     IGAVVLDGAVVEEGAQVGAGALVPPGKVVPAGWLVMGVPAKPVRRMSPEELDDILRNA
                     TDYVELWHRDYRSGVGS"
     gene            complement(745..1527)
                     /locus_tag="VFX87_08820"
     CDS             complement(745..1527)
                     /locus_tag="VFX87_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4995057.1"
                     /translation="MPTPDETLVTREGPVVTLTFNRPEARNAMTWGMYDRLYEVCEEV
                     DADDSVRVLVLRGAGGKAFVAGTDISQFKVFDRAEDGIAYERDGDRRMARLESVRKPV
                     IAQIEGFAVGGGFRIATACDLRIATPDARFGAPIARTLGNCLSMAAYASFVDVFGPSR
                     LKELIFTARLLTAEEANRAGYVHEIVPAAQIEARVTALAQQIAGHAPITLWVTKESIR
                     RLGASRTLPDDEDLIATTYMSADFHEGVRAFLDKRKPRWSGT"
     gene            1623..2237
                     /locus_tag="VFX87_08825"
     CDS             1623..2237
                     /locus_tag="VFX87_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX4995058.1"
                     /translation="MTRPAGLILDYGNVLTHAQDQTWMDGAALRLGADRAAFRAAYWQ
                     HRHAYDADLSAVEYWGRVLNTSRPDAGALDAVDLAWLIEADIASWSVYHDEVWALAAA
                     FRRAGGRTAFLSNSGPEVMARVRARWPLEARCDAVLISCEVGLSKPDPRIYRLCLDRL
                     GLPAPETLFVDDRADNIEGAARVGLRTFRFEGPDALERLRALVG"
BASE COUNT          323 a          906 c          818 g          354 t
ORIGIN      
        1 gatcagggcc accgcggcgc cgatcatcca gatccacggc gggtcctgca ccaaccggag
       61 tagcacgatc agggcgacgc ccgccagcag caggagccac gccttcagga agagcaccca
      121 gtgcagccgc gtcttgtaca gcacgcgctc cccgggcagg agatgccgct ccacgtagcc
      181 caccggcgtc agcccctctc ggccccggcg ggggaactat cgtatttcaa gacccgaccc
      241 cggagcggta gtcgcggtgc cagagctcga cgtagtccgt cgcgttgcgc aggatgtcgt
      301 ccagctcctc ggggctcatc ctgcgcaccg gcttggccgg cacccccatg acgagccagc
      361 ccgccggcac caccttgccc ggcggcacca gcgcgcccgc ccccacctgc gcgccctcct
      421 ccaccaccgc cccgtccagc accaccgcgc cgatgccgat gcgcgagttg ttcatcacgg
      481 tgcaggcgtg gaccaccgcg cggtggccga tcgtgacgtt gtcgcccacg atgcacggga
      541 actgcggtgt tacgtggagc accgagttgt cctgcacgtt ggagttgcgg ccgagcttga
      601 cgtagttgtc ctgatcgcca cgcagcaccg tgccgggcca cacgctggcc cccgcctcga
      661 tggtcacgtc gccgatgacc tgggccgccg ggtccacgta ggcctccggg tgcacccgcg
      721 gcttgcggcc gggcacctcg cggatcacgt accgctccac cggggcttgc gcttgtcgag
      781 gaaggcgcgc actccctcgt ggaagtcggc gctcatgtag gtggtcgcga tcaggtcctc
      841 gtcgtccggc agggtccggc tcgcccccag gcggcggatg gactccttgg tgacccacag
      901 cgtgatcggc gcgtgcccgg cgatctgttg ggccagcgcg gtcacccgcg cctcgatctg
      961 cgccgccggc acgatctcgt gcacgtagcc cgcccggttt gcctcctcgg cggtgagcag
     1021 ccgcgcggtg aagatcagct ccttgagccg cgagggcccg aacacgtcca cgaagctcgc
     1081 gtaggcggcc atcgagaggc agttgccgag ggtgcgcgcg atcggcgcgc cgaagcgcgc
     1141 gtccggcgtg gcgatgcgca ggtcgcaggc ggtggcgatg cggaagccgc cgcccaccgc
     1201 gaagccctcg atctgggcga tgaccggctt gcgtacggac tcgagccgcg ccatgcgccg
     1261 gtcgccgtcg cgctcgtagg cgatgccgtc ctcggcccga tcgaagacct tgaactggct
     1321 gatgtccgtc cccgccacga aggccttgcc gccggcgccg cggaggacca gcacccgcac
     1381 cgagtcgtcg gcgtccacct cctcgcagac ctcgtagagg cgatcgtaca tcccccacgt
     1441 catggcgttg cgcgcttcgg ggcggttgaa cgtcagggtg accaccggcc cctcgcgagt
     1501 caccagcgtt tcgtccggcg tcggcacggc gcgcgcctcc ctctcccggc gtgatgagtc
     1561 gccgcatcgt agcacggccg ctccgctttg tcggagcgcc cggctcgcat agcatcgagc
     1621 gcatgacccg ccccgccggc ctcatcctcg actacggcaa cgtgctcacc cacgcgcagg
     1681 accagacgtg gatggacggg gccgcgctcc ggctcggcgc cgaccgcgcc gccttccggg
     1741 ccgcctactg gcagcatcgg cacgcctacg atgccgatct ctccgccgtg gagtactggg
     1801 gccgcgtgct gaacacgtcc cgccccgacg ccggcgcgct ggacgcggtg gatctcgcgt
     1861 ggctgatcga ggccgacatc gcctcgtgga gcgtctacca cgatgaggtc tgggcgctcg
     1921 ccgccgcgtt tcgccgggcc ggcggccgca ccgccttcct gtcgaacagt ggccccgagg
     1981 tgatggcgcg ggtgcgcgcg cgctggcccc tcgaggcgcg ctgcgacgcg gtgctcatct
     2041 cgtgcgaggt cgggctctcc aagccggatc cgcgcatcta tcggctctgc ctggaccggc
     2101 tcgggctgcc cgcgccggag accctcttcg tggacgatcg cgccgacaac atcgagggcg
     2161 ccgcgcgcgt cggcctgcgg accttccgat tcgagggccc cgacgcgctc gagcggctgc
     2221 gagccctggt cggctgagcg gtcccgtccg cgcggatggc caacctgggc ggtgtaccgc
     2281 gcgggccttc tgctcgtcgc cgggcaggct gacgcgcagt ggcggtacac ggacgacaag
     2341 ggcacgggcc gagtcacgca atacgagctc gacgtcccca cgccgtaccg cgacggggcc
     2401 c
//
LOCUS       DASUFA010000235         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_630109, whole genome shotgun sequence.
ACCESSION   DASUFA010000235 DASUFA010000000
VERSION     DASUFA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630109"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFX87_08830"
     CDS             complement(<1..625)
                     /locus_tag="VFX87_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX4995059.1"
                     /translation="MPGVDAHPLVIGHRGASADAPENTIAAFELALEQGADGIELDVH
                     LSADEQPVVIHDFTLERTTDGTGPVSGHRVRELKRLDAGGWRARRFRGQRVQTLQEVL
                     ERFRDRARFWIELKGGSALYPGIEERVVSMVEIYDVVDRVLVQSFDHATIARVRVLNR
                     EIPVGALVAQAPFDPGLLRPEAVSAICPDAHVLTEALLGEIWRAGLGC"
     gene            661..1305
                     /locus_tag="VFX87_08835"
     CDS             661..1305
                     /locus_tag="VFX87_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4995060.1"
                     /translation="MRTPVIDVARPVIRLGARIYFRIRFEGVHHIPRSGPLLIVPNHV
                     TFADPVLATIPVRRPVHYMAWNALFRVRGLAWLIRRLRAFPVDIDGADAGATRAAVRL
                     LRAGEAVMIFPEAGRSLDGRVQRFRLGAFRLACSLGVPIMPVTIIGAHECWPPGRRLP
                     RPGRLSIVYHPVIAPPEAADVKGAARDLAHRVHGVVASALPAHQRPDAARDAPA"
     gene            1349..1840
                     /gene="aroQ"
                     /locus_tag="VFX87_08840"
     CDS             1349..1840
                     /gene="aroQ"
                     /locus_tag="VFX87_08840"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX4995061.1"
                     /translation="MARRVASAILVLHGPNLNLLGTREPAVYGRVTLAEVNRAVQRHA
                     ERRGARAVCRQSNHEGELVDWIQAAARDGFRGIVFNPGALSHYSIALRDAVASVRVPV
                     VEVHLSNIHAREEFRHHSVVAAAAVGQISGFGPGSYLLGLDALLGASSALGGRRTAAR
                     RRP"
     gene            1912..3006
                     /locus_tag="VFX87_08845"
     CDS             1912..3006
                     /locus_tag="VFX87_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4995062.1"
                     /translation="MTSPDAPSSEAGLPESPGIDERALIQRCIAGDAAAFEPLVEKYR
                     QRVWRLAYQVLHDREEAWDVAQEAFVRAFHSLSSFRGQSAFYTWLFRITVNVATDRHR
                     QRGAQARAFGPERVTEEEWARSMPDPGRGPEQEATRAEQRERIRRGLDALPPKARTII
                     MLSDVEGLSYREIAEVLNCPIGTVMSRLHNARKQLKGLLGPMLALILWLGLTLGLANP
                     AQAQQIIRFGVRIMEASSPSRAAESEGALRNPAPEAETDERMKRILPRLRTLFRYTDY
                     ITLDRQRVQGPLGTRQRFAVPGERWLEVTPDQLQGDHVRMQVRLLRGVHPEVNAAIQA
                     APGAPAVLGGPPYGNGVLIIILWANPNPAP"
     gene            3013..>4409
                     /locus_tag="VFX87_08850"
     CDS             3013..>4409
                     /locus_tag="VFX87_08850"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019422140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX4995063.1"
                     /translation="MSEQRYRQERARIEQGHVKYREKLRDEGKLFVRDRLKLLLDPGT
                     EFQEDFLFARNQEPDTPADGVVTGVGTVGGRAVCVMANDYTVKAGSWGEKTIQKIVRI
                     QERAQRLQVPMIYLVDAAGGRISEQIKIFPGRFHAGRIFYNEVQLSGVVPQVCVLFGP
                     SPAGSAYLPALTDCVIMVDGKASLYVGSPRMVEMAIGEKTTLEELGGARMHCTVSGCG
                     DVLAASDEEAIELCRRYLSYLPGSFRERPAPVEQVGPKPGRSIEEIVPYDQRKWFDMY
                     EVIDRVIDAGSFFEIKRLFASEIIVGFARLGGRAVGVVANQPKVKGGVLMVDSADKAA
                     RFISLCNAFNVPLVYLADVSGFMVGTKVERAGIIRHGAKMVFATSQATVPKICVVVRK
                     CYGAGLYAMCGPAFEPDAALALPQGQVAIMGPEPAVNAVYYNKIMELPESERAAYVRE
                     KREEFVQDVDIYKLAS"
BASE COUNT          619 a         1576 c         1555 g          659 t
ORIGIN      
        1 agcagcccag ccccgcccgc cagatctccc cgagcagcgc ctcggtgagg acgtgcgcgt
       61 ccgggcagat cgcgctgacc gcctccggcc gcagcaggcc cgggtcgaac ggggcctgcg
      121 ccaccagggc ccccaccggg atctcacggt tgagcacccg cacccgggcg atggtcgcgt
      181 gatcgaagga ctgcacgagg acccggtcca ccacgtcgta gatctcgacc atcgagacca
      241 cgcgctcctc gatgccggga tagagcgccg agccgccttt cagctcgatc cagaagcggg
      301 cgcggtcccg gaagcgctcc aggacttcct ggagcgtctg cacgcgctgg ccgcggaacc
      361 gccgggcccg ccagccgccc gcgtccaggc gcttgagctc gcggacgcgg tggccgctca
      421 ccggccccgt gccgtcggtg gtccgctcca gcgtgaagtc gtggatgacc accggctgct
      481 cgtcggccga gaggtgcacg tcgagctcga tgccgtcggc gccctgctcg agcgccagct
      541 cgaaggcggc gatggtgttc tccggcgcgt cggccgaggc gccgcggtga ccgatcacga
      601 ggggatgcgc gtccactccg ggcactgtcg gtctcaggca gagcgtggca ctatcgggcc
      661 gtgcgcacgc ccgtgatcga cgtcgcccgg ccggtcatcc ggctcggagc gcgcatctac
      721 ttccgcatcc ggttcgaagg cgtccaccac attccccgct cgggccctct cctcatcgtg
      781 ccgaaccacg tcacgtttgc cgacccggtc ctcgcgacca ttccggtgcg ccgacccgtc
      841 cactacatgg cctggaacgc cttgttccgc gtgcgcggcc tcgcgtggct gatccgccgc
      901 ctgcgcgcct tcccggttga catcgacggc gccgacgcgg gcgccacccg ggccgcggtc
      961 cggcttctcc gcgcgggcga ggcggtcatg atcttccccg aggccggccg gagcctcgac
     1021 gggcgggtcc agcgcttccg cctcggcgcg ttccgcctgg cctgctcgct cggcgtgccc
     1081 atcatgcccg tgaccatcat cggcgcgcac gagtgctggc cgcccggtcg ccgcctgccg
     1141 cggcccggcc gcctctccat cgtctatcac ccggtgatcg cgccgccgga ggcggccgac
     1201 gtgaaggggg ccgcgcggga cctggcccat cgcgtgcatg gggtcgtcgc gtccgccctg
     1261 cccgcgcacc agcgcccgga cgccgcgcgg gacgcgcccg cctgaggcgc gagcattata
     1321 cgggcgtccg gtgtattctc tcccttcaat ggcacgtcgc gtcgcgtccg ccatcctcgt
     1381 cctccacggc ccgaatctca acctcctcgg cacccgcgag ccggcggtgt acggccgggt
     1441 cacgctcgcg gaggtgaacc gcgccgtgca gcggcacgcg gagcgccgcg gagcacgggc
     1501 agtctgccgg cagtccaacc acgagggcga gctggtggac tggatccagg cggcggcgcg
     1561 cgacggcttc cgcggcatcg tcttcaaccc gggcgcgctg tcgcactact cgatcgcgct
     1621 acgcgacgcg gtggcctcgg tgcgcgtgcc ggtggtggag gtgcacctct cgaatatcca
     1681 tgcccgcgag gagttccggc accattcggt ggtcgcggcg gccgcggtcg ggcagatctc
     1741 cggcttcggg cccggcagct acctgctcgg cctggatgcc ctcctgggcg cctcgtccgc
     1801 gctcggcggc cggcggacag ccgcgcggcg ccggccgtga atgcgggcgc ccttcctgcc
     1861 gtcctacccc cgatcgtata aggggcgcga gggccacggc cgggtccggc cgtgacgtcg
     1921 cccgacgctc catccagcga agccgggctc ccggagtccc ccgggatcga cgagcgcgcg
     1981 ctcatccagc ggtgcatcgc cggagacgcc gcggcgttcg agccgctggt cgagaagtac
     2041 cgtcagcggg tgtggcgtct ggcctaccag gtgttgcacg accgcgagga ggcgtgggac
     2101 gtggctcagg aggcgttcgt gcgggcgttt cattcgctgt catcgttccg cggtcagtcg
     2161 gcgttctaca cctggctctt ccgcatcacg gtcaacgtgg ccaccgatcg gcaccggcag
     2221 cgcggcgctc aggcgcgcgc cttcggcccg gagcgggtga ccgaggagga atgggcccgc
     2281 agcatgcccg atccgggacg cgggccggag caggaagcga cgcgggcgga gcagcgcgag
     2341 cgcatccggc gggggctcga tgccttgccg cccaaggccc gaacgattat catgttgagt
     2401 gacgtggaag gcctctcgta tcgcgagatc gccgaggtgc tcaactgccc gatcgggacc
     2461 gtgatgtcca ggctgcataa cgcgcgaaag cagctcaagg ggctcctggg accgatgctc
     2521 gccctgatcc tctggctcgg actgaccctg ggcttggcca atccggccca ggctcagcag
     2581 atcatccgct tcggggtccg catcatggag gcctccagcc cctcccgcgc cgccgagagc
     2641 gagggcgcgc tcaggaatcc cgcgcccgag gcggagaccg acgagcgcat gaagcgcatt
     2701 ctcccgcggc tgcggacgct cttccgctac accgactaca tcacgctcga ccgccagcgc
     2761 gtccaggggc cgctcggcac ccggcagcgg ttcgcggtgc cgggagagcg gtggctcgag
     2821 gtcacgcccg atcagctcca gggcgaccac gtgcgcatgc aggtacggct cctgcggggc
     2881 gtgcatcccg aggtgaacgc ggcgatccag gccgcgccgg gcgcccccgc ggtgctcggg
     2941 ggcccgccgt acggcaacgg cgtgctgatc atcatcctct gggccaatcc caaccccgct
     3001 ccctagacca gcgtgagcga gcagcgctac cggcaggaac gcgcccgcat cgagcagggc
     3061 cacgtcaagt accgggagaa gctgagggac gaaggcaagc tcttcgtccg cgatcgcctg
     3121 aagctgctcc tggatcccgg caccgagttc caggaggatt tcctcttcgc gcgcaaccag
     3181 gagccggata cccccgccga cggcgtggtc accggggtgg ggacggtcgg cggccgcgcc
     3241 gtgtgcgtca tggccaacga ctacacggtg aaggcgggct cgtggggcga gaagacgatc
     3301 cagaagatcg tgcgcatcca ggagcgcgcg cagcggctgc aggtgccgat gatctacctc
     3361 gtggatgcgg cgggcgggcg catctcggag cagatcaaga tcttcccggg ccgattccac
     3421 gccggccgca tcttctacaa cgaggtccag ctctccgggg tggtgccgca ggtctgcgtg
     3481 ctcttcggcc cgtcgccggc cggctccgcc tatctgcccg cgctgaccga ctgcgtgatc
     3541 atggtggacg gcaaggcgag cctgtacgtg gggtctccgc gaatggtcga gatggccatc
     3601 ggcgagaaga cgacgctcga ggagctgggt ggagcccgga tgcactgcac ggtgtcggga
     3661 tgcggcgacg tgctcgccgc ctccgacgag gaggcgatcg agctctgccg gcgatacctc
     3721 agctacctgc ccggctcgtt ccgcgagcgg cccgcgccgg tggagcaggt cggcccgaag
     3781 ccgggccggt cgatcgagga gatcgtcccg tacgaccagc gcaagtggtt cgacatgtac
     3841 gaggtgatcg accgcgtcat cgacgccggc tccttcttcg agatcaagcg gctcttcgcg
     3901 tcggagatca tcgtgggctt cgcccgcctc ggcgggcgcg cggtgggtgt ggtggccaac
     3961 cagcccaagg tcaagggcgg cgtgctgatg gtggactcgg ccgacaaggc ggcgcggttc
     4021 atctcgctct gcaacgcctt caacgtcccg ctcgtctacc tggccgacgt gtcgggcttc
     4081 atggtgggca ccaaggtgga gcgcgccggg atcattcgcc acggggccaa gatggtcttc
     4141 gccacctcgc aggccaccgt gcccaagatc tgcgtggtcg tgcgcaagtg ctacggcgcg
     4201 gggctctacg cgatgtgcgg gcccgccttc gagccggacg ccgcgctggc gctgccgcag
     4261 gggcaggtgg ccatcatggg gccggagccg gccgtcaatg cggtctacta caacaagatc
     4321 atggagctgc cggagagcga gcgtgcggcc tacgtccggg agaagcgcga ggagttcgtc
     4381 caggacgtgg acatctacaa gctcgcctc
//
LOCUS       DASUFA010000236         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1185054, whole genome shotgun sequence.
ACCESSION   DASUFA010000236 DASUFA010000000
VERSION     DASUFA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185054"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VFX87_08855"
     CDS             <1..1558
                     /locus_tag="VFX87_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4995064.1"
                     /translation="ERAAFFEGSYGTGDTGHADLLLTEALGPFRLSLEGTYFTTDGYK
                     VVNASRRGAIDVDAGSEHVTFNGRAELVLSPDLSVFVRGTYYEESRENGTPLQLNNTD
                     SGSFATGMRARTGDGSEWTGTVFADFQAFRSAFSSQAADRNSETLALDQRVPSTSVGA
                     AASWSRTFGAHRLLAGADFRWIDGETDERVYVAGAFARTRVAGGEQQIYGLFVQDVYR
                     PIPMVELVAGIRGDYWRAYNGFRRDTPPPAGIPARQTYSDIDRIGVSPRVAALVHATP
                     TTDVRASAYQGFRVPTINELYRVFRVRNDVTAANEHLKPERLTGGEVGVEQRWGPFTG
                     RATVFWNEVENLIANVTLTNRLPDCPVGTTCRQRQNLDRARMRGVETELEVRPARDWQ
                     IIAGYVFTDTTVLDAPQQVALEGKRLAQVPRHAASVTVRYDNRALFTAAATVRFVGRQ
                     FEDDLNTLPLGSYALVDLFLSRRLTRWAEVFVGIENLLDTTYSTGRTSEGVVSIGAPF
                     QVRGGARLSF"
     gene            1558..1968
                     /locus_tag="VFX87_08860"
     CDS             1558..1968
                     /locus_tag="VFX87_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEX4995065.1"
                     /translation="MGPLRIRLGPLTFTARWEEAAAPRTCAAFRRLLPYRQRIIQARW
                     SGEAGWIPLGEFDLGVSAENATSEPAPGALLFYPAGVSETEILFPYGPTRFASVHGPL
                     AGNHFLTIEEGRARLPEVGRLLCWDGAQDITFEA"
     gene            complement(1952..2731)
                     /locus_tag="VFX87_08865"
     CDS             complement(1952..2731)
                     /locus_tag="VFX87_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4995066.1"
                     /translation="MGFMHERGLGMPPDPAEAVAWYRRAALQGDVHAQFRLGFLHAYG
                     RGVDRDDLQAADWYGRAAAQGNRAAQAALDDLRAEGRLPTPGGSRDALLLRSAADRGG
                     ATAQYQLGVRYATGEGVAQDAQQAAYWYRLAAEQGVAPAQYQLGLRYANGDGVTRDYA
                     EAAKWYRRAAEQGVAFAQFNLGVRYINGQGVARDPVQAYQWFSVAAQGLLGKEADTAR
                     QARDSVKAQLTPEQLARAEEMVRSWRAKPEAPGAPVATPRR"
     gene            complement(2905..3186)
                     /locus_tag="VFX87_08870"
     CDS             complement(2905..3186)
                     /locus_tag="VFX87_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995067.1"
                     /translation="MLADRYVKSVLTVIALALVAIAAHLWQGQLRPAAVAAQTAAKYE
                     IGIPKAWGKLVGFSNNNLLLESSDGLLRIVDVEGREPEYPKIKVQVRWK"
     gene            complement(3217..>3572)
                     /locus_tag="VFX87_08875"
     CDS             complement(3217..>3572)
                     /locus_tag="VFX87_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995068.1"
                     /translation="GLAARISVGNRQEMVFRETAATIAPYDGHHVAVYISNFSGPHAW
                     LVEHGLLTEESNPYQYRFQVIADPESGRRLSEIEHEVRCATHPMYMRPLINRNAAQRQ
                     ATYVRGRDQFVPGLL"
BASE COUNT          493 a         1324 c         1247 g          508 t
ORIGIN      
        1 cgagcgcgcg gccttcttcg agggcagcta cggcaccggc gacaccgggc acgccgacct
       61 gctgctgacc gaggccctcg ggcccttccg cctgagcctc gagggcacct acttcacgac
      121 cgacggctac aaggtcgtga acgcctcgcg ccggggcgcc atcgacgtgg acgcgggctc
      181 cgagcacgtc acgttcaatg ggcgcgcgga gctcgtcctc tcgccggatc tctcggtgtt
      241 cgtgcgcggc acctactacg aggagagccg ggagaacggc accccgctgc agctcaacaa
      301 taccgattcc ggctcgttcg cgaccgggat gcgcgcgcgc accggggacg gcagcgagtg
      361 gaccggcacc gtcttcgccg acttccaggc tttccgcagc gccttcagct cccaggccgc
      421 cgatcggaac tcggagacgc tcgccctcga ccagcgcgtg ccgagcacgt cggtgggtgc
      481 cgcggcgtcg tggagccgga ccttcggggc gcaccggctg cttgcgggcg ccgacttccg
      541 gtggatcgac ggcgaaaccg acgagcgcgt ctacgtcgcg ggcgccttcg cccgcacgcg
      601 agtggcgggc ggcgagcagc agatctacgg gctcttcgtg caggacgtct accgaccgat
      661 cccgatggtc gagctggtcg cgggcatccg cggcgactac tggcgcgcct acaacggctt
      721 ccggcgtgat accccgccgc ccgccggcat ccccgcccgc cagacctact ccgacatcga
      781 ccggatcggc gtgagcccgc gcgtggccgc gctcgtgcac gccacgccca ccaccgacgt
      841 gcgcgcctcc gcctaccagg gcttccgggt gccgaccatc aacgagctgt accgtgtgtt
      901 ccgcgtgcgc aacgacgtga cggcggccaa cgagcacctg aaacccgagc ggctcaccgg
      961 cggcgaggtg ggggtggagc agcgctgggg accgttcacc ggccgcgcca ccgtcttctg
     1021 gaacgaggtg gagaacctga tcgccaacgt gaccctcacc aaccgcctgc ccgattgccc
     1081 ggtggggacc acgtgccggc agcggcagaa cctcgaccgc gcgcgcatgc gcggggtcga
     1141 gaccgagctc gaggtgcgcc cggcccgcga ctggcaaatc atcgccggct acgtcttcac
     1201 cgacaccacg gtgctggacg ccccgcagca ggtggccctc gagggcaagc gcctggccca
     1261 ggtgccacgg cacgccgcct ccgtcacggt gcgctacgac aatcgggcgc tcttcaccgc
     1321 ggcggccacc gtgcgcttcg tgggccgcca gttcgaggac gacctcaaca cgctgccgct
     1381 cggcagctac gcgctcgtgg atctcttcct ctcgcgccgc ctcacccgct gggccgaggt
     1441 cttcgtcggc atcgagaacc tgctcgacac cacctacagc accgggcgca ccagcgaggg
     1501 ggtcgtctcc atcggggcgc cgttccaggt gcgcggcggg gcgcgtcttt cgttctgatg
     1561 ggtcccctcc ggatccggct ggggccgctg acgttcaccg cgcgctggga ggaggccgcc
     1621 gcgccgcgca cctgcgccgc gttccgccgc ctcctgccct accggcagcg aatcatccag
     1681 gcgcgctgga gcggcgaggc gggctggatc ccgctcggcg agttcgacct gggcgtgagc
     1741 gcggagaacg cgacgagcga gcccgcgccc ggcgcgctgc tcttctaccc cgccggggtc
     1801 agcgagaccg agatcctgtt cccctacggt cccacgcgct tcgcgagcgt gcacggcccg
     1861 ctggccggca atcacttcct cacgatcgag gagggccgcg cgcggcttcc cgaggtgggc
     1921 cggctcctct gctgggacgg cgcccaggac atcaccttcg aggcgtagcg accggcgcgc
     1981 ccggagcctc cggcttcgcc cgccagctcc gcaccatctc ctcggcgcgc gcgagctgct
     2041 cgggggtgag ctgagccttg accgagtccc gcgcctggcg cgcggtgtcc gcctccttgc
     2101 cgaggaggcc ctgggccgcg acgctgaacc actggtaggc ctgcaccggg tcgcgcgcca
     2161 cgccctggcc attgatgtag cgcacaccca ggttgaactg cgcgaaggcc acgccctgct
     2221 cggcggcccg ccggtaccac ttcgccgcct ccgcatagtc acgggtgacg ccgtcgccgt
     2281 tggcgtagcg cagcccgagc tggtactgcg cgggcgcgac cccctgctcg gcggccagtc
     2341 ggtaccagta cgcggcctgc tgggcgtcct gggccacccc ctcaccggtc gcgtagcgca
     2401 cgccgagctg gtactgagcg gtggcgccac cgcgatcggc cgcgctccgc agcagcagcg
     2461 catcacgcga gcctcccggg gtggggagcc ggccttccgc gcgcaggtcg tcgagcgcgg
     2521 cctgggccgc ccgattgccc tgcgcggcgg cgcggccgta ccagtcggcg gcctgcaggt
     2581 cgtcacggtc caccccgcgg ccgtaggcgt ggaggaagcc caggcggaac tgagcgtgca
     2641 cgtcgccctg gagcgcggcg cggcgatacc aggcgacggc ttcggcggga tccggcggca
     2701 tgcccagccc acgctcgtgc atgaagccca cgaggaactg ggtggcggcg ttcccgccct
     2761 cggcgagcgg acgccactcg cggtacgcgg tcgcgtaatc gccgcggtcg taagcgcgac
     2821 gaccgtcctc gaacgccgcg tcgacgggga ccgccccgag caggagcgtg agaacggcca
     2881 gcgcgagggc gccgcgccag cggcttactt ccagcgcacc tgcaccttga tcttggggta
     2941 ctccggctcc cggccttcca cgtccacgat gcggagcagg ccgtcgctgc tctcgagcag
     3001 gaggttgttg ttggagaagc ccacgagctt cccccacgcc ttcgggatgc cgatctcgta
     3061 cttggcggca gtctgggcgg ccaccgcggc cgggcggagc tggccctgcc agaggtgggc
     3121 ggcgatggcc acgagggcga gggcgatcac cgtgagcaca ctcttgacgt atcggtcggc
     3181 gagcatgcga atcctcctcg gcgccgtaga ccggcgctag agcagcccgg gcacgaactg
     3241 gtcccgtccc cgcacgtagg tggcctggcg ctgggccgca ttgcggttga tcagcgggcg
     3301 catgtacatg gggtgagtcg cgcagcggac ctcgtgctcg atctccgaga gacgccgtcc
     3361 gctctccggg tcggcgatga cctggaagcg gtactgatac ggattgctct cctcggtcaa
     3421 gagcccgtgc tccaccagcc acgcgtgcgg gcccgagaag ttggagatgt acaccgcgac
     3481 gtgatggccg tcgtacggag cgatcgtggc ggcggtctcg cggaacacca tctcctgccg
     3541 gttcccgacg ctgatgcgcg cggccagacc gg
//
LOCUS       DASUFA010000237         5052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_863979, whole genome shotgun sequence.
ACCESSION   DASUFA010000237 DASUFA010000000
VERSION     DASUFA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863979"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VFX87_08880"
     CDS             <1..1447
                     /locus_tag="VFX87_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX4995069.1"
                     /translation="RDVTYGEVALRANRVGNALVARDVQIEQRVLLALPDRPEFAEAF
                     WGAMKIGAVPVPVSDGLPAEELAFLVQDSRARAVIASEAAATEILKIRAQCPALESVI
                     VVGGRRRGALEYDRLLEKASPELAAADTSRDDVALWLYTSGSTGRPKAAVHLHHDLVY
                     AAERVGRGTFGIAADDVVFSASKLHFAFGLGNSLYFPALVGAASVLVPERPEAERIFE
                     LIQAERPTMLFAVATIYRRLLQVEGAERRFDLSSLRICVSSGEALPAGIFHAWKSRFG
                     HELLDVLGSTEALHDYIANRPGEARAGSSGRVIPGYEARLVDGQDRPVAPGFVGQLLI
                     KGDSTAAYYWNRHERTRATMQGEWLRTGDMFYQDADGYFYFCGRQDDMLKVGGMWVSP
                     VEIEACLGEHPAVLEAAVIGRGDADGLTKAHAFCVLREGERGTEALAAALQALVRQRL
                     AGYKSPRWVDFVAELPKTATGKVQRFALRRR"
     gene            1449..2246
                     /locus_tag="VFX87_08885"
     CDS             1449..2246
                     /locus_tag="VFX87_08885"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995070.1"
                     /translation="MRVVVEGVSKTFADRRGRDVVALSQVDFAVEPEEFVALLGPSGC
                     GKSTLLNIIGGLLPASSGRVYFEGEPREGRPMAATVFQEFALFPWRTVRANVEFGLEE
                     LGVSGFERAERTRHYLDMTGLAGFETKYPHQLSGGMRQRVGIARALAVEPAVLLLDEP
                     FSALDAQTRTLMMEELLGIWTRTRQSILYVTHNIQEAVFMADRVIVLSRRPGEVLDTV
                     PIRLSRPRGEHQQGDPAFIQASDRIWGLIKSQARAALLEGDALTERG"
     gene            2246..3043
                     /locus_tag="VFX87_08890"
     CDS             2246..3043
                     /locus_tag="VFX87_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995071.1"
                     /translation="MAMERFIVDDRTAFLRRPDYRTARVLALAGLVILWELITRTGFV
                     SSLFLPSPLGVLAEGWDMARSGQLLVHLATSLERLAWGFAIGALAGISVGVAVGFFSL
                     ADAVVQPLIAATFPIPKIALLPLLILWLGIGEASKVAVVALGVFFPMAINTYSGVRQA
                     DPLLIRAAVSFGAGRWSVIRKVILPSALPMVFAGLRLGAGIALLLLVAAEQIAADAGI
                     GFLILQSGNLMETTKLMVGIVVLSLLGVLSHWGLSGLERAVIRWRNT"
     gene            3059..4090
                     /locus_tag="VFX87_08895"
     CDS             3059..4090
                     /locus_tag="VFX87_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995072.1"
                     /translation="MRRLSRLVLALAAIAGGICLSPGAEAQSPTPLKIGVLKLTSSAP
                     VFVGVEKGYFKEFGVEPELVYFQAAAPIAAALAAGQLDVGATGLTAALYNIVLGGEQL
                     WIVADKGREWPGYPLTGIVVQKDLWDGGVRTIKDLKGRRIGVTQVGSTFHYTLGRVLE
                     KDGLSLADVKIAPLQAMPAMMEALKGKQVDAILVPQPFPGTAEAQGFGKILAWAGDLY
                     PWQNVAVFYSQKLAANRPRAVAFMKGYVKASRYYFDAALAQKDGRLVPGANYEDVVQI
                     TAKYTGARPEVIRLGFPFQDRNGRLLVTDIERQMAWWTANGFMKKTLPLRSIVDTSFV
                     EEAVKALDR"
     gene            4170..>5052
                     /locus_tag="VFX87_08900"
     CDS             4170..>5052
                     /locus_tag="VFX87_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX4995073.1"
                     /translation="MSAPFGKVLIANRGEIAVRVIRACRELGIRTVAVFSEADRESLH
                     VLLADEAVPIGPPPATESYLVIDKLIAAARATGAEAVHPGYGFLAENAKFAQACLDAK
                     LTFIGPPPAAIRAMGDKMAARRVAIKMGVPVVPGTEQPVSDDAEAVRVAERVGYPVML
                     KAAMGGGGKGMRLVRTAAELTGALRAARSEAGAAFGDAAVYIERYVEEPRHIEIQVLA
                     DAHGGVVYLGERECSIQRRHQKLVEESPSPFVTPEMRRRMGEAACRVAAAVGYVNAGT
                     VEFLVDRDRNFYFLEMNT"
BASE COUNT          671 a         1743 c         1851 g          787 t
ORIGIN      
        1 ccgcgacgtc acctacggcg aggtcgccct ccgcgccaac cgcgtcggca acgcactcgt
       61 ggcccgcgac gtgcagatcg agcagcgcgt gctgctggcc ctgccggacc gccccgagtt
      121 cgccgaggcg ttctggggcg cgatgaagat cggcgcggtg ccggtgccgg tgagcgacgg
      181 gctgcctgcc gaggagctgg cgtttctcgt ccaggacagc cgcgcccgcg cggtcatcgc
      241 ctccgaggcc gccgccaccg agatcctcaa gatccgcgcg cagtgccccg cgctggagag
      301 cgtcatcgtg gtgggcggcc ggcggcgggg cgcgctcgag tacgaccggc tgctggagaa
      361 ggcctcgccc gagctggccg cggcggacac gagccgcgac gacgtggcgc tctggctcta
      421 cacctcgggc tccaccgggc ggcccaaggc ggccgtgcac ctgcatcacg acctggtcta
      481 cgcggcggag cgggtggggc gcgggacctt cggcatcgcg gcggacgacg tggtcttctc
      541 ggcgtcgaag ctccacttcg cgttcgggct cggcaactcg ctctacttcc cggcgctggt
      601 gggcgcggcg tcggtgctgg tgccggagcg gccggaggcc gagcggatct tcgagctgat
      661 ccaggccgag cgtcccacca tgctgttcgc ggtggccacg atctatcgcc ggctcctgca
      721 ggtggaaggg gcggagcggc ggttcgatct ctcgtcgctg cggatctgcg tctcctccgg
      781 cgaggcgctg cccgccggga tcttccacgc ctggaagtcg cgcttcggcc acgagctgct
      841 ggacgtgttg ggctccaccg aggcgctgca cgactacatc gccaaccggc cgggggaggc
      901 gcgggccggg tcgagcgggc gggtgatccc gggctacgag gcgcggctgg tggacggcca
      961 ggaccggccg gtggcgcccg ggttcgtggg gcagctcctg atcaagggcg actccaccgc
     1021 ggcgtactac tggaaccgcc acgagcgcac ccgggccacc atgcagggcg agtggctgcg
     1081 caccggcgac atgttctacc aggatgccga cggctacttc tacttctgcg gccgtcagga
     1141 cgacatgttg aaagtcggcg gcatgtgggt gtcgccggtc gagatcgagg cgtgcctggg
     1201 cgagcacccg gcggtgctgg aggccgcggt gatcggacgc ggggacgccg acgggctcac
     1261 caaggcgcac gccttctgcg tgctgcgcga gggcgagcgc ggcaccgagg cgctcgccgc
     1321 cgcgctgcag gcgctggtgc gccagcgact ggccggctac aagtcgccgc ggtgggtgga
     1381 cttcgtggcc gagctgccca agaccgccac cggcaaggtc cagcggttcg ccttgcggcg
     1441 gcgctaggat gcgcgtcgtg gtcgaagggg tgtccaagac gtttgccgac cggcggggtc
     1501 gcgacgtcgt cgcgctgagc caggtggact tcgcggtgga gcccgaggag ttcgtggctc
     1561 tgctgggccc ctcgggctgc ggcaagtcca cgctgctcaa cattatcggg gggctcctgc
     1621 ccgccagctc ggggcgcgtc tacttcgagg gtgagccccg cgagggccgg ccgatggcgg
     1681 ccaccgtgtt ccaggagttc gcgctctttc cctggcgcac ggtgcgggcc aacgtggagt
     1741 tcggcctgga agagctgggc gtctcgggtt tcgagcgggc cgagcgcacg cggcattacc
     1801 tcgacatgac cggcctcgcc ggcttcgaga ccaagtaccc gcaccagctc tcgggcggca
     1861 tgcgccagcg cgtggggatc gcgcgggccc tcgcggtgga gccggcggtg ctgctgctgg
     1921 acgagccgtt ctcggcgctg gacgcgcaga cccgcaccct gatgatggag gagctcctcg
     1981 gcatctggac ccgcacgcgc cagtccatcc tctacgtcac gcacaatatc caagaggcgg
     2041 tgttcatggc cgaccgggtg atcgtgctct cgcggcggcc gggcgaggtg ctcgacacgg
     2101 tgccgatccg gctctcccgc ccgcgcggcg agcaccagca gggcgatccc gccttcatcc
     2161 aggccagcga tcgcatctgg gggctgatca agtcgcaggc gcgggccgcg ctgctcgagg
     2221 gcgacgcgct gacggagcgc ggctgatggc gatggagcgc ttcatcgtgg acgaccgcac
     2281 cgccttcctg cggcggcccg actaccgcac ggcgcgcgtc cttgcgctgg ccggcctggt
     2341 catcctctgg gagctcatca cgcgcaccgg cttcgtgtcg tcgctcttcc tgccgtcgcc
     2401 gctcggcgtg ctcgcggaag gctgggacat ggcgcgctcc ggccagctcc tcgtgcacct
     2461 ggcgaccagc ctcgagcgcc tggcctgggg cttcgcgatc ggggcgctgg ccggcatcag
     2521 cgtgggcgtg gcggtgggct tcttctccct ggccgacgcg gtggtgcagc cgctcatcgc
     2581 ggccacgttc ccgatcccca agatcgcgct gctgccgctg ctgatcctct ggctcggcat
     2641 cggcgaggcc tccaaggtcg cggtggtcgc gctcggcgtc ttcttcccga tggccatcaa
     2701 cacctactcg ggtgttcgcc aggccgatcc gctgctgatc cgggccgccg tgtccttcgg
     2761 ggcggggcgg tggagcgtga tccgcaaggt catcctgccg tcggccctcc ccatggtctt
     2821 cgccgggctc cgactcggcg ccggcatcgc gctgctcctg ctggtggccg ccgagcagat
     2881 cgcggccgac gcgggcatcg gcttcctgat tctccagtcc ggcaacctga tggaaaccac
     2941 caagctcatg gtcggcatcg tcgtgctctc gctgctcggc gtcctctcgc actggggcct
     3001 ctccgggctc gagcgcgcgg tcatccgctg gcgtaacacc tgatcggagg ctctcatcat
     3061 gcgacgactc tcccggctgg ttctggccct cgccgcgatc gcgggcggca tctgcctctc
     3121 gcctggcgcg gaggcgcaat cgccgacgcc gctcaagatc ggcgtgctca agctcacgtc
     3181 ctcggcgccc gtcttcgtgg gcgtcgagaa aggctacttc aaggagttcg gcgtcgagcc
     3241 cgagctggtg tacttccagg cggccgcgcc catcgccgcc gcgctggccg cgggccagct
     3301 cgatgtcggg gccaccggcc tcaccgccgc gctctacaac atcgtgctgg ggggcgagca
     3361 gctgtggatc gtggccgaca agggccgcga gtggccgggg tacccgctga ccggcatcgt
     3421 ggtgcagaaa gatctgtggg acggcggcgt gcggaccatc aaggacctca agggtcggcg
     3481 gatcggggtg acccaggtcg gctccacgtt tcactacacc ctcgggaggg tgctggagaa
     3541 ggacggcttg agcctcgccg acgtgaagat cgcgccgctc caggccatgc cggcgatgat
     3601 ggaggcgctc aagggcaagc aggtggacgc catcctggta ccgcagccct ttcccggcac
     3661 cgccgaggcc cagggcttcg ggaagatcct ggcgtgggcg ggggatctct acccctggca
     3721 gaacgtggcg gtgttctact cgcagaagct ggccgcgaac cggccacggg ccgtcgcttt
     3781 catgaagggc tacgtcaagg cctcccgcta ctacttcgac gcggccctgg cgcagaagga
     3841 cggccgcctc gtgcccgggg ccaactacga ggacgtcgtg cagatcacgg ccaagtacac
     3901 gggggcccgg cccgaggtca tcaggctcgg cttccccttc caggatcgca acggtcgcct
     3961 gctcgtgacc gacatcgagc ggcagatggc ctggtggacg gccaacgggt tcatgaagaa
     4021 gacgctgccg ctccggtcca tcgtggacac ctccttcgtg gaggaggccg tgaaggcgct
     4081 cgacaggtag aaagcccctc gccctggccc tctcctccca gggggagagg gtggggtgag
     4141 ggggtagttc ctgctatcat gctcgcaacg tgagcgcccc gttcgggaag gtcctcatcg
     4201 cgaatcgcgg cgagatcgcc gtccgggtca tccgggcgtg ccgcgagctg ggcatcagga
     4261 ccgtcgccgt cttctccgag gccgatcgcg agtccctcca cgtgctgctc gcggacgagg
     4321 cggtgccgat cggcccgccg ccggccaccg agagctacct cgtgatcgac aagctgatcg
     4381 ccgcggcccg cgccaccgga gcggaggcgg tgcatccggg ctacggcttc ctggccgaga
     4441 acgcgaagtt cgcccaggcc tgcctggacg cgaagctgac cttcatcggg ccgccgcccg
     4501 ccgcgatccg ggcgatgggc gacaagatgg ccgcccgtcg cgtcgccatc aagatgggcg
     4561 tgccggtggt gccgggcacc gagcagccgg tctccgacga cgcggaggcc gtgcgggtgg
     4621 ccgagcgcgt gggctatccg gtcatgctga aggccgcgat gggcggcggc ggcaagggca
     4681 tgcgcctggt gcgcaccgcc gccgagctga cgggcgccct gcgggccgcg cgctcggagg
     4741 cgggcgccgc gttcggcgac gccgcggtct acatcgagcg ctacgtggag gagccgcgcc
     4801 acatcgagat ccaggtgctg gccgacgcgc acggcggggt cgtgtacctg ggagagcgcg
     4861 agtgctcgat ccagcgccgg caccagaagc tggtggagga gagcccgtcg cccttcgtga
     4921 cgcccgagat gcggcgtcgg atgggcgagg ccgcctgccg ggtggccgcc gcggtcggct
     4981 acgtcaacgc gggcaccgtc gagttcctgg tggaccgcga ccgcaacttc tacttcctcg
     5041 agatgaacac cc
//
LOCUS       DASUFA010000238         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1185312, whole genome shotgun sequence.
ACCESSION   DASUFA010000238 DASUFA010000000
VERSION     DASUFA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185312"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFX87_08905"
     CDS             <1..282
                     /locus_tag="VFX87_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX4995074.1"
                     /translation="DSVVKRLKETGVVGGLENSNVRIVEEATVPGAPIRPRKAWNLGV
                     SLVAGLVLAFGVAFAIEYLDTTVKTPDDVERYLGLSVIGVVPSFHARRR"
     gene            282..1028
                     /locus_tag="VFX87_08910"
     CDS             282..1028
                     /locus_tag="VFX87_08910"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX4995075.1"
                     /translation="MATRESRSHSHPHHPDPVLITLSEPKSAASEAFRTLRTNIQFAG
                     LDQPCRSIVVTSAGPEEGKTTSAANFGVVSAQAGSRVCVVDSDLRRPTLHRLFKLSNA
                     RGLTTALLEGLSFAEVAQPTAVPNLSILTSGPLPPNPAELVGSHRMRECLRAAAEAFD
                     LVLCDSPPLMAVGDASALAAQCDGVIFVIRVGKTPNDVLRRVVDQIEAVKGRILGVVL
                     NRVDPRRDGYYYYYQAYQRYYGADEGGAKR"
     gene            1072..1797
                     /locus_tag="VFX87_08915"
     CDS             1072..1797
                     /locus_tag="VFX87_08915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4995076.1"
                     /translation="MTLDLWGTLFLDGPGSDERYRRQRLAGMQAVLAGAGIAVSPAQL
                     DRGHAAASRWLGELWQMNRDVSVREYVVALLEAVDAALPGRLSAETMAALISAYASPA
                     LMAPPAVDPGARVALETLAAEGARLAVISNTMRTPGEVVRQVLDKAGLLPLFRVLTFS
                     DECGIRKPASGIFLRTLKDLDVPPEAAVHVGDDAILDVEGARDAGMAAIQVTPDGRAT
                     APVKPDAVIRHLGELPAALRRLR"
     gene            1797..>3294
                     /locus_tag="VFX87_08920"
     CDS             1797..>3294
                     /locus_tag="VFX87_08920"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX4995077.1"
                     /translation="MAIRLTGPRARVYLIAASVIVMVLALFLWAPDFLQAVEVRLYDL
                     HFTLRGAQPQTAERIVIAAIDEKSLAALGRWPWPRSLMADLVRKLSADGARVIAVDIL
                     LSEPELSGELRAATQLSERLRTLGLAGSSAGSAVQRELDAFAREADRDRQLAEAVGAS
                     GRVILPIVFEVGPDRSGPPPEPSGAAFKSALVSFRHRDERALYPPPAARSATPPIPVL
                     AAVAQTLGHVTMLADPDGTTRWEAVAFEHEGHYYPSLAVQAVSLATGVEPTRLTLDFG
                     RALAVGSVSVPLDPRDRMLINYAGPGGTFRHLSAVDLLTGKVPPDAVRDRIVFVGATA
                     AGTYDLRVTPTSPIMPGVEKHANVAGNILSGRFLQRPDWVELVELAGILILPLVLAWL
                     LPRLRPAVSVGAVALVWAMLFGSVHLTFRGGLWLPVVYPTLALGLTFVGITVYRLLTE
                     ERQRLWTKRAFQQFVSPEVVERLMDNPAALQFGGEMRNLTVLFSDIRDF"
BASE COUNT          429 a         1169 c         1175 g          521 t
ORIGIN      
        1 gactcggtcg tcaagcgcct caaggagacc ggagtggtcg gagggctcga gaacagcaac
       61 gtccggatcg tcgaggaggc aaccgtgccc ggtgcgccga tccgaccgcg gaaggcatgg
      121 aacctgggcg tgagcctcgt ggccgggctg gtcctcgcct tcggcgtggc cttcgccatc
      181 gagtacctcg acaccacggt gaagacgccc gacgatgtgg agcgctacct cgggctgtcg
      241 gtgatcgggg tcgttccctc gttccacgcc aggcggcgct gatggccacg cgcgagagta
      301 gatcccattc tcatccgcac catcccgatc cggtcctcat cacgctgagc gagcccaagt
      361 ccgccgcgtc cgaggccttc cggacgctcc gcaccaatat ccagttcgcc gggctggatc
      421 agccctgccg gagcatcgtg gttacctcgg ccggccccga ggagggcaag accacctcgg
      481 cggccaactt cggggtcgta tccgcgcagg ccgggtcccg cgtgtgcgtg gtcgattccg
      541 acctccggcg cccgacgctg caccgcctct tcaagctgag caacgcgcgc gggctgacca
      601 cggccctgct ggagggcctg tcgttcgccg aggttgccca gcccacggcg gtgcccaacc
      661 tctcgatcct gaccagcggg ccgctgccgc ccaacccggc ggagctggtg ggctcccacc
      721 ggatgcggga gtgcctgcgg gccgccgccg aagcgttcga tctcgtcctg tgtgacagcc
      781 cgcccctgat ggccgtcggc gacgcgtcgg cgctcgccgc gcagtgcgac ggggtgatct
      841 tcgtgatccg ggtgggcaag acgcccaacg atgtcctgcg ccgcgtggtc gatcagatcg
      901 aagcggtcaa gggcagaatc ctgggtgttg tcctgaaccg ggtggacccg cggcgcgacg
      961 gctactacta ttactaccag gcctaccagc gctactacgg agccgatgag ggcggcgcga
     1021 agcgctgaga cgcgccggcc gggacggcca acgggccgcg tgatccgcgc ggtgacgctc
     1081 gatctgtggg gcacgctgtt tctcgacggg cccgggagcg atgagcgcta caggcgccag
     1141 cggctggccg ggatgcaggc cgtgctggcg ggtgcgggca tcgcggtatc tccggcccag
     1201 ctcgatcgag gccacgccgc cgcctcccgc tggctcggcg agctctggca aatgaaccgg
     1261 gacgtctcgg tccgggaata cgtcgtggcg ctcctcgagg ccgtggatgc cgcgctgccg
     1321 ggccggctga gcgccgagac gatggcggcc ctgatcagcg cctacgcgag cccggccctg
     1381 atggccccac cggccgtcga tccgggcgcg cgcgtcgcgc tcgagacgct cgccgccgag
     1441 ggcgcgcgcc tcgcggtcat ctcgaacacc atgcggacgc cgggtgaggt cgtgcgacag
     1501 gtgctcgaca aggcggggct cctcccgctg ttccgcgtgc tgaccttctc cgacgaatgc
     1561 gggatcagga agcccgcctc ggggatcttc cttcgcacgc tgaaggatct ggatgtgccg
     1621 cccgaggctg cggtgcacgt gggcgacgac gcgatcctcg atgtcgaggg cgctcgcgac
     1681 gccgggatgg cggccatcca ggtgactccc gacgggcgcg ccacggcccc cgtgaagccg
     1741 gatgccgtca tccggcacct cggcgagctg ccggctgcgc tgcggcggct ccgctgatgg
     1801 caatccgtct gaccggcccg cgcgcgcggg tctatctgat cgccgcctcg gtcatcgtga
     1861 tggtgctcgc cctgttcctc tgggcgcccg acttcctgca ggcggtggag gtccggctct
     1921 acgatctcca cttcaccctg cggggcgctc agccgcagac ggcggaacgg atcgtcatcg
     1981 ccgccatcga cgagaagagc ctggccgcgc tgggtcgctg gccctggccg cgctcgctca
     2041 tggccgacct cgtccggaag ctctcggcgg acggcgcccg cgtcatcgcg gtcgatattc
     2101 tgctgagcga gccggagctg agcggcgagc tccgggcggc cacgcagcta tcggaacggc
     2161 tccggacgct ggggctggcc ggcagctccg cggggtcggc ggtccagcgc gagctcgatg
     2221 cgttcgcccg cgaggccgat cgcgatcgtc agttggccga ggccgtcggg gccagtggcc
     2281 gcgtgatcct gcccatcgtg ttcgaggtcg gtcccgaccg gtccgggccg cctcccgagc
     2341 cgtcgggcgc ggccttcaag tcggctctgg tgagcttccg tcaccgcgac gagcgggcgc
     2401 tgtaccctcc gccggcagcc cggagcgcga ccccgcccat ccccgtgctg gcggccgtgg
     2461 cccagacgct cgggcacgtg acgatgctgg ccgaccccga tggcacgacg cgctgggagg
     2521 ccgtggcgtt cgagcacgag ggccactact acccgtcgct ggcggtccag gcggtgagcc
     2581 tggcgacggg ggtggagccg acacggctca cgctcgactt cggccgggcc ctggcggtcg
     2641 gatcggtctc ggtgcccctc gatccccgcg accggatgct gatcaactac gcgggccccg
     2701 gcgggacctt ccggcacctg tccgcggtgg atctgctgac cgggaaggtc ccgccggatg
     2761 ccgtgcggga ccgcatcgtc ttcgtgggcg ccaccgcggc cgggacgtac gatctgcgcg
     2821 tcacgccgac cagcccgatc atgcccgggg tggagaagca cgccaacgtg gcgggcaata
     2881 tcctgagcgg ccgattcctc cagcgcccgg actgggtgga gctggtcgag ctggccggca
     2941 tcctgatcct cccgctggtg ctggcgtggc tgctgccccg gctccggcca gcggtgagcg
     3001 tgggcgccgt ggcgctcgtc tgggcgatgc tcttcggctc cgtccatctc acgttccgcg
     3061 gcgggctctg gctccccgtg gtctatccga cgcttgccct ggggctgacc ttcgtcggga
     3121 tcacggtgta ccgtctgctc accgaggagc gacagcggct gtggacgaag cgtgcgttcc
     3181 agcagttcgt cagtcccgag gtcgtggagc ggctcatgga caatcccgcc gcgctccagt
     3241 tcggcggcga gatgcggaac ctcaccgtgc tcttctcgga catccgcgac ttca
//
LOCUS       DASUFA010000239        10389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1430825, whole genome shotgun sequence.
ACCESSION   DASUFA010000239 DASUFA010000000
VERSION     DASUFA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430825"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFX87_08925"
     CDS             <1..191
                     /locus_tag="VFX87_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995078.1"
                     /translation="IVDAPIGSQGRTALEALRVGDLFGVSMILIDGQIKIGRSRNTPP
                     ATRAAEVVKGQGPATGGH"
     gene            191..760
                     /locus_tag="VFX87_08930"
     CDS             191..760
                     /locus_tag="VFX87_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEX4995079.1"
                     /translation="MKVRKLLTVVEEIQEDGGRRVEKPLRKVAAIAVIANPFAGRFVE
                     DLQELVKTGEELGDLLGRRAVTALGGPVHSYGKAAIVGTDGEYEHAAAILHPTLGTPF
                     RALVHGGKAIIPSAKKLGAPGTSIDVPLHYKDAAFVRTHFDAMEVRVHDAPRANEILV
                     ALVVTDGGRPHPRVGGLTVAEAKKEDGLR"
     gene            787..1773
                     /locus_tag="VFX87_08935"
     CDS             787..1773
                     /locus_tag="VFX87_08935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4995080.1"
                     /translation="MKFGLRLPSFALGPKTASLAEMGAYLRRAEDLGFDCAVSIDHLL
                     LTPPAYACTWLEPVALLSALAGVTRTIKLGTMVLVLPLRNPAYFAKEWATLDLLSGGR
                     SILGVGVGWHEEEFALMGVPHKERGRRMDEMLEAVTALWVGDNVTYEGKYYRFRNLTI
                     DPKPAQTPHPPIWIGGGSQPFEKVYGQTVTNIDPVLRRIAKYAKTWVPHSSATADMVK
                     GDWEKIQRFMVEFGRKPGDMSKVYSNFVWVLKKGEKPETAVPHFKTYSGMDLPYWREF
                     YLLGEAEELAEKIRAKVANLGGCEYIVLNPLTWGIDQLELLANEVLPRVARP"
     gene            1821..4121
                     /locus_tag="VFX87_08940"
     CDS             1821..4121
                     /locus_tag="VFX87_08940"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4995081.1"
                     /translation="MEDPRLLAGQGRFLDDISLPGLLHAAFVRSPHAHAALRSVDTTA
                     ARAMAGVELVLTGRGLDGVVAPLEPRLEASGFASTAWPVLPTARVRFVGEAVAAVAAT
                     SPYAAVDGCALVQVEYDPLPAVPDVDTARDPRAPRLHPEHGSNVLFERRGGQGDVDGA
                     FAAAAVVVRETFTHARCSAAPLEGRGVIARWEGDVLTLWTGSQVPHVFRTGVARAFGV
                     PESRVRVIVPDTGGGFGQKMHVMPEDLAVAALARRIRRPVKWIETRRENLAAAAHARE
                     ARVEIEAAADRAGVLLALRARLWSDAGAYHIYPLTAALEPLGTASILPGPYRTPAYAF
                     HLTALATTKPPLGAYRGVGMTMGAFVMERTLDLLADRLGLDPAEIRRRNLIPRDAYPF
                     TSATGFVYDSGDYPKALEQALTLAGYDRLTRERENGRARGRLLGVGLACYTEYTGIGS
                     ETYRRRGMADVPGHEAARVEMVADGSVTCYVSFPSQGQGHATTTAQLVADQLGVALEA
                     VTVRQPDTDTAPGGTGTFASRGAIAQSGAADAAAGTVRRKLLAIAGRLLEASPADLLL
                     RDGRVSVRGMPDRGVSVAEVARLACAPPPGGLPPGMEPGLEATQSFDPPGPTFSGAVH
                     VAGVEVDRDTGHVTVREYTVVEDCGPVINPMIVEGQIHGAVAQGIGEALGERLVYDES
                     GQLATGTLMDYALPVAGALPFFAIGHVETPSPLTPGGYKGMGEGGTIGAPAAIANAVA
                     DAVKPLGVAITALPILPAALRRDGGR"
     gene            4348..5127
                     /locus_tag="VFX87_08945"
     CDS             4348..5127
                     /locus_tag="VFX87_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX4995082.1"
                     /translation="MTTLGRVAIAAALWAGITGAGVGAAGAAELRVFSAGAVRAIVTE
                     LAQAFQQDTGHTVTLSFGPVGVTRAKLASAEPVDVVIMTDVAIDDVAKQGAVVPGSRA
                     DLARTGMGLGVREGAPKPDIATSEAFKRTLLSAKSIVYVDPKQGATSGIHFASVLDRL
                     GIADAVKGKTQLVPGGYPAERVASGEAEVVVHQISEIVPVKGVVLVGPLPPDLQKVTV
                     YSAGLAARSAAPETARAFIAFLTRPAFKAKFAAAGLDYRQP"
     gene            5165..6022
                     /gene="lgt"
                     /locus_tag="VFX87_08950"
     CDS             5165..6022
                     /gene="lgt"
                     /locus_tag="VFX87_08950"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX4995083.1"
                     /translation="MFASPGAIAFQLGPLQIRWYGILMATAILVGFWLAHRRAVREGL
                     PADQLVRAAQWAVVAGLVGARLYEVVFNWDYYGRFPEKIIAVWEGGLAIHGGLIVGPL
                     VGALLAWRWGVPVLRALDVIAPSMILGQAIGRWGNFFNEEAFGAPTDLPWKLYISPPH
                     RPLEFRQLDFFHPTFLYESLWNLLVFVLLVRWIRPRVGRYPGAVFFAYIGLYSVGRFS
                     IEAVRLDSFWIGSFRVGQLASVAGVLVAAAGIRWAMQRAPIDGTTAAPAPDPATGRGP
                     GKRGRRPPG"
     gene            complement(5976..7238)
                     /locus_tag="VFX87_08955"
     CDS             complement(5976..7238)
                     /locus_tag="VFX87_08955"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX4995084.1"
                     /translation="MTDTSEFDSELHRTALGQLDRVASQLALDSDIHERLRFPRRALV
                     VSVPIRMDRGDTEVFIGYRVHHNTALGPTKGGLRYDLGVNLGEVTALAMLMSWKCALM
                     GLPYGGAKGGVRCNPRELSPRELEHLTRRYTAEIIHMIGPDLDIPAPDLGTDEQTMAW
                     MMDTYSMTQGKSVPGVVTGKPIIVGGSAGRREATGRGIVFVLYQAVKGLGQELRGKKI
                     IVQGFGNVGSVAARLLWNDGCTIAGVSDVKGGVWNPGGLDIRQLQDHVAQTGSVVGFP
                     GGEPVSNEAILEQPCDVLIPAAVDSVIHARNADRIKAHIVAEAANGPTTPEADVILRD
                     RGVTVLPDILCNAGGVVVSYFEWVQGLQYYFWKESEIISRLQEIMARSFTSVWRTAQK
                     NGVDLRTGALMEGISRVADAHVSRGLYP"
     gene            complement(7242..9404)
                     /gene="glgX"
                     /locus_tag="VFX87_08960"
     CDS             complement(7242..9404)
                     /gene="glgX"
                     /locus_tag="VFX87_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX4995085.1"
                     /translation="MADPPRTRPGRPYPLGATWDGAGVNFALFSEHATAVALCFFDER
                     DPGKEVRQIRIEQRTDQVWHAYVPGVRPGTLYAYRVNGPYEPALGHRFNPAKALLDPY
                     AKAIAGPLEWQDALFGYRIGDPAGDIRPHDRDSGPYLPKSVVVDGTFDWEGDRPPLTR
                     WSRTVIYEVHVKGFTKLHPEVPPELRGTYAGLASDAAVRHLTELGVTAVELLPIHHSV
                     TEKYQADRGFTNYWGYNSIGFFCPDSRFASSGQRGQQVDEFKRMVKRLHRAGIEVILD
                     VVYNHTAEGNHLGPTLCFRGIDNAAYYRLSPEDPRFYVDYTGCGNTLNMRHPRTIQLL
                     MDSLRYWVLEMHVDGFRFDLAAALARELHDVDRLSAFFDVIHQDPVISQVKLIAEPWD
                     LGQGGYQVGNFPVGWTEWNGKYRDTIRRYWKGDEGQVAELAYRLTGSSDLYEMGGRRP
                     YASVNFVTAHDGFTLADLVSYNDKHNLANGENNRDGTDDNNSWNCGVEGPTGDPRIVA
                     IRERQLRNFLATLFLSQGIPMLLGGDEMGRTQRGNNNSYCQDSDLSWFHWPPTETGQR
                     LIEFTRRLIRLKHANPVFHRRMFFQGRRIHGSALKDLAWFRPDGHEMTDEEWQNSFGR
                     CLGLRLAGDAIEEVDDLGEPIVGDTFLILLNAHHEPVPFVLPAHESRVSWDPVLDTRD
                     WDGDVARPSLRTGEQYVLEGRSLAVLRLGRTAHPAQGA"
     gene            complement(9418..>10389)
                     /gene="glgB"
                     /locus_tag="VFX87_08965"
     CDS             complement(9418..>10389)
                     /gene="glgB"
                     /locus_tag="VFX87_08965"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX4995086.1"
                     /translation="VDAVASMIYRDYSRKAGEWIPNEYGGRENLEALAFLRKLNEVVY
                     GGQPGASTIAEESTAWPQVSRPVHLGGLGFGFKWNMGWMHDILEFMQHEPVHRKYHHN
                     QLTFGLLYAWTENFVLPLSHDEVVYGKRSLARKMPGDDWQRFANLRLLYAFMWAYPGK
                     KLLFMGGEFGQSDEWSHDRALDWSLLDMGPYHRGVQRLVKDLNHVYRNEPSLHEVDFE
                     PAGFQWMDCSDWEQSIVTFCRFSRNQERLLLCACNFTPVARRGYRVGVPRPGFYRETV
                     NSDASVYGGGDVGNAGGVASEPVPWHGQPHSVVLTLPPLGALWLTPA"
BASE COUNT         1429 a         3577 c         3811 g         1572 t
ORIGIN      
        1 gcatcgtgga cgcgccgatc ggctcccagg gccggaccgc gctcgaggcg ctccgggtcg
       61 gcgatctctt cggcgtctcc atgattctca tcgacgggca gatcaagatc gggcggagcc
      121 gcaacacccc gcccgccacg cgcgcggcgg aggtcgtgaa gggccagggc cccgccacgg
      181 gaggtcactg atgaaggtcc gcaagctgct cacggtggtg gaggagatcc aggaggacgg
      241 cggccgccgc gtggagaagc ctctgcggaa ggtggcggcg atcgcggtga tcgcgaatcc
      301 gttcgcgggc cgcttcgtcg aggacctgca ggagctggtc aagaccggcg aggagctggg
      361 cgacctgctc ggccggcgcg cggtgacggc gctggggggc ccggtgcact cctacggcaa
      421 ggcggcgatc gtggggacgg atggtgagta cgagcacgcc gcggccatcc tgcatcccac
      481 gctcggcacc ccgttccgcg cgctcgtgca cggcggcaag gcgatcatcc cgtccgccaa
      541 gaagctgggc gcgcccggca cctccatcga cgtcccgctc cactacaagg acgcggcctt
      601 cgtgcgcacc cacttcgacg cgatggaggt gcgggtccac gacgcgcccc gcgccaacga
      661 gatcctggtg gccctcgtcg tcaccgacgg cgggcgcccg cacccccgcg tcggcggtct
      721 caccgtcgcc gaggcgaaga aagaggacgg tctccgatga gggccgaagg cccgaagagg
      781 cccggcatga aattcggcct gcgcctcccg agcttcgcgc tcggccccaa gaccgcctcg
      841 ctcgccgaga tgggcgccta cctgcggcgg gcggaagacc tgggcttcga ctgcgcggtg
      901 tccatcgatc atctcctgct gaccccaccc gcctacgcct gcacctggct cgagccggtg
      961 gcgctgctct cggccctggc cggcgtcacc cgcacgatca agctgggcac catggtgctg
     1021 gtcctcccgc tccgcaaccc ggcctacttc gccaaggagt gggccacgct cgacctgctc
     1081 tcgggcgggc gcagcatcct gggcgtaggg gtcggctggc acgaggagga gttcgcgctc
     1141 atgggcgtgc cgcacaagga gcgcgggcgg cggatggacg agatgctgga ggcggtgacc
     1201 gcgctctggg tgggcgacaa cgtcacctac gagggcaagt actaccggtt ccgcaacctc
     1261 acgatcgatc ccaagcccgc gcagaccccg cacccgccca tctggatcgg cggtggcagc
     1321 cagcccttcg agaaggtcta cggccagacc gtcaccaaca tcgacccggt gctgcggcgc
     1381 atcgccaagt acgcgaagac ctgggtcccg cactcctccg ccaccgccga catggtcaag
     1441 ggcgactggg agaagatcca gcgcttcatg gtcgagttcg gccgcaagcc cggcgacatg
     1501 agcaaggtct actcgaactt cgtgtgggtg ctgaagaaag gcgagaagcc ggagaccgcg
     1561 gtcccgcact tcaagaccta ctccggcatg gatctgccgt attggcggga gttttatctc
     1621 ctgggagagg cggaggagct ggccgagaag atccgggcca aggtcgcgaa cctcggcggc
     1681 tgtgagtaca tcgtgctgaa cccgctcacc tgggggatcg accagctcga gctgctggcc
     1741 aacgaggtgt tgccccgcgt ggcgaggccc tgacgctgag cgccccgccg ccgcgctatc
     1801 tcggcgcgca cgtcaagcgg gtggaggatc cccgcctgct ggcggggcag ggccggttcc
     1861 tcgacgacat ttcgctgccc ggcctgctcc acgccgcctt cgtgcggagc ccacacgccc
     1921 acgccgcgct gcgctccgtg gacacgacgg ctgcccgcgc catggccggg gtggagctgg
     1981 tgctgaccgg acgcggtctc gatggcgtgg tcgcccccct cgagccgcgc ctcgaggcct
     2041 ccggcttcgc gtcgaccgcc tggcccgtgc tgcccaccgc ccgcgtccgc ttcgtcggag
     2101 aggcggtggc ggcggtggcc gccacctcgc cctacgccgc cgtggacggt tgcgcgctcg
     2161 tccaggtcga gtacgatccg ctgcccgcgg tacccgacgt ggacaccgcg cgcgatcccc
     2221 gcgccccgcg gctgcacccc gagcacggct ccaacgtgct cttcgagcgc cggggcggcc
     2281 agggcgacgt ggacggggcc ttcgcggccg cggccgtcgt ggtccgcgaa acgttcaccc
     2341 acgcgcgctg ctcggccgcg cccctcgagg gccgcggggt gatcgcgcgc tgggagggcg
     2401 acgtgctcac gctctggacg ggcagccagg tgccgcacgt cttccggacc ggcgtcgccc
     2461 gcgcgttcgg cgttcccgag tcgcgggtgc gcgtgatcgt gcccgatacc ggcggcggct
     2521 tcgggcagaa gatgcacgtg atgcccgagg acctggcggt ggccgcgctg gcccgccgca
     2581 tccggcgtcc ggtgaagtgg atcgagaccc gacgcgagaa cctggccgcg gcggcccacg
     2641 cccgcgaggc gcgtgtggag atcgaggcgg ccgccgatag ggccggcgtg ctgctggcgc
     2701 tgcgggcgcg cctctggtcg gacgcggggg cgtatcatat ctacccgctc accgccgcgc
     2761 tcgagccgct gggcacggcc tccatcctgc ccgggcccta ccgcacgcct gcctacgcct
     2821 tccatctcac ggcgctcgcc accaccaagc cgccgctcgg cgcctaccgc ggggtgggga
     2881 tgaccatggg cgccttcgtg atggagcgca cgctggacct gctcgccgat cggctgggcc
     2941 tcgacccggc cgagatccgc cgccgcaacc tgatcccccg cgacgcctac cccttcacct
     3001 cggccaccgg cttcgtctac gatagcggcg actatccgaa ggcactcgag caggcgctca
     3061 cactggccgg ctacgaccgg ctcacgcgcg agcgcgagaa cgggcgggcg cggggccggc
     3121 tgctcggcgt cggcctggcc tgctacacgg agtacacggg catcggctcc gagacgtatc
     3181 gccgccgcgg catggcggac gtgccggggc acgaggccgc gagggtcgag atggtggcgg
     3241 acggcagcgt cacctgctac gtgtcgtttc cgtcccaggg ccaggggcat gccacgacca
     3301 ccgcgcagct cgtcgccgat cagctcggcg tggcgctcga ggcggtgacg gtgcggcagc
     3361 ccgatacgga taccgccccg ggcggcaccg gcaccttcgc gagccgcggc gcgatcgccc
     3421 agagcggcgc ggccgatgcg gcggcgggca cggtacggcg caagctgctg gcgatcgcag
     3481 gcaggctgct cgaggcgagt cccgcggacc tgctgctgcg ggatgggcgc gtgagcgtgc
     3541 ggggcatgcc cgatcgtggt gtgtcggtgg ccgaggttgc gcgcctggcc tgcgcacccc
     3601 cgcccggcgg cctgccgccg ggtatggagc cggggctcga ggcgacccag tccttcgacc
     3661 ccccggggcc caccttctcg ggcgcggtgc acgtggcggg tgtcgaggtg gatcgcgaca
     3721 ccgggcacgt cacggtgcgc gagtacacgg tcgtcgagga ctgcgggccc gtgatcaatc
     3781 cgatgatcgt cgagggtcag attcacggcg cggtggccca gggcatcggc gaggcgctcg
     3841 gcgagcgtct cgtctacgac gagagcggac agctcgccac cggcacgctg atggactacg
     3901 cgctgccggt ggccggcgcg ctgccgttct tcgccatcgg ccacgtggag acgccgtcgc
     3961 cgctgacgcc cggcggctac aagggcatgg gtgagggcgg cacgatcggc gcgcccgccg
     4021 ccatcgcgaa cgcggtggcc gacgccgtga agccgctcgg cgtggcgatc accgcgctgc
     4081 cgatcctgcc cgccgccctc cgacgcgatg gtgggcgcta gcgccgccgc gcccgatccg
     4141 gaatcgcccc cgcccgcgcg tcgggccgtc tctggtcgca cggggggctg gccctcgtgc
     4201 tcgcggtggc ggccgcgctg cgcctctggc gactcgatca gaacgggttc gggaggggac
     4261 agcgggtgcc gggcgggccg cgcggcggca gctctacgat ctgaatccgg aggcgggtct
     4321 ggtcccggcc tccgaaaggg agaagacatg acgactctgg ggcgggtggc gatcgcggcg
     4381 gcattgtggg cgggcatcac gggcgcgggg gtcggcgcgg ctggcgccgc cgagctccgg
     4441 gttttcagcg cgggggcggt gcgggccatc gtcaccgagc tggcgcaggc cttccagcag
     4501 gacacgggtc acaccgtgac gctctcgttc ggccccgtcg gcgtcacgcg cgccaagctg
     4561 gcctctgccg agccggtgga cgtggtcatc atgaccgacg tggcgatcga cgacgtggcc
     4621 aagcagggcg cggtggttcc gggcagccgc gccgatctcg cgcgcacggg catggggctc
     4681 ggcgtgcgcg agggcgcgcc gaaacccgac atcgccacgt cggaggcgtt caagcgaacg
     4741 ctcctgtcgg cgaagtcgat cgtctacgtg gatcccaagc agggcgccac cagcggcatc
     4801 cacttcgcga gcgtgctgga ccggctcggc atcgcggacg cggtgaaggg caagacccag
     4861 ctcgtgccgg gtggttatcc cgccgagagg gtcgcgtccg gcgaggccga ggtggtggtg
     4921 caccagatca gcgagatcgt gccggtgaag ggcgtggtgc tggtgggccc gctgccgccc
     4981 gatctccaga aggtgaccgt ctactcggcg ggtctggccg cgcgcagcgc cgcgccggag
     5041 accgcgcggg ccttcatcgc gttcctgacc cggcccgcct tcaaggccaa gttcgccgcc
     5101 gcggggctcg actaccgcca gccctgacga cccggccggg cggggccgtg ctatcgtgca
     5161 gagcgtgttc gcctcgcccg gcgccatcgc gttccagctc gggccgctcc agatccggtg
     5221 gtacggcatc ctcatggcca ccgcgatcct ggtgggcttc tggctcgccc accggcgtgc
     5281 cgtgcgggag gggctgcccg ccgaccagct cgtgcgcgcc gcccagtggg ccgtggtggc
     5341 gggcctggtc ggcgcgcgcc tgtacgaggt ggtcttcaac tgggactact acggccggtt
     5401 cccggagaag atcatcgcgg tctgggaagg cgggctcgcg attcacggcg ggctcatcgt
     5461 cggcccgctc gtcggcgcgc tgctcgcgtg gcggtggggc gtgccggtgc tccgcgccct
     5521 cgacgtcatc gcgcccagca tgatcctggg ccaggccatc ggccgctggg ggaacttctt
     5581 caacgaggaa gccttcgggg cgcccaccga cctgccgtgg aagctctaca tctcgccgcc
     5641 gcaccgaccg ctcgagttcc ggcagctcga cttcttccac cccacgttcc tctacgagtc
     5701 gctgtggaat ctgctcgtgt tcgtgctgct cgtacggtgg atccgcccgc gggtcggtcg
     5761 gtatcccggg gcggtcttct tcgcctacat cgggctctac tcggtgggcc ggttctccat
     5821 cgaggcggtg cgtctcgaca gcttctggat cgggtcgttc cgggtgggac agctcgcgag
     5881 cgtggcgggc gtgctggtgg cggcggcggg gatccggtgg gccatgcagc gggccccgat
     5941 cgacgggacc acggccgcgc cggctcccga tccggctacg ggtagaggcc ccgggaaacg
     6001 tgggcgtcgg ccacccggct gatgccttcc atcagcgcgc cggtccggag gtccacccca
     6061 ttcttctggg cggtgcgcca cacgctcgtg aacgaccgcg ccatgatctc ctgcagccgc
     6121 gagatgatct cgctctcctt ccagaagtag tactgcaggc cctgcaccca ttcgaagtac
     6181 gacacgacca cgccgcccgc gttgcacagg atgtccggga gcacggtcac cccgcggtcg
     6241 cgcaggatca cgtcggcctc gggcgtggtg ggcccgttgg cggcctcggc cacgatgtgg
     6301 gccttgatgc ggtcggcgtt gcgcgcgtgg atgaccgagt cgaccgcggc cgggatcagc
     6361 acgtcgcagg gctgctccag gatggcctcg ttgctgaccg gctcgccgcc cgggaagccc
     6421 accacgctgc cggtctgggc cacgtggtcc tggagctggc ggatgtcgag gccgccggga
     6481 ttccacaccc cgcccttgac gtcgctgacg cccgcgatcg tgcacccgtc gttccacagg
     6541 aggcgcgcgg ccacgctgcc cacgttgccg aagccctgca cgatgatctt cttgccccgc
     6601 agctcctggc ccaggccctt gaccgcctgg tagagcacga agacgatgcc gcgcccggtg
     6661 gcctcgcgcc ggcccgcgga gccgcccacg atgatcggct tgccggtgac gaccccgggc
     6721 acgctcttgc cctgggtcat cgagtaggtg tccatcatcc acgccatcgt ctgctcgtcg
     6781 gtgccgaggt cgggggcggg gatgtcgaga tcggggccga tcatgtggat gatctcggcg
     6841 gtgtagcgcc gggtgaggtg ctccagctcg cgcggggaca gctcgcgcgg gttgcagcgc
     6901 acgccgccct tggccccgcc gtagggcagg cccatcaggg cgcacttcca gctcatcagc
     6961 atcgcgagcg cggtgacctc ccccaggttg acgcccagat cgtagcgcag gccgcccttg
     7021 gtagggccga gcgcggtgtt gtggtgcacc cggtagccga tgaacacctc ggtgtcgccg
     7081 cggtccatcc ggatcggcac cgagaccacg agggcccggc gcgggaagcg tagccgctcg
     7141 tggatgtcgg agtcgagcgc gagctggctc gcgacgcgat cgagctggcc cagcgcggtg
     7201 cggtgcagct cggaatcgaa ttcggacgtg tcggtcatcg gtcaggctcc ttgcgcggga
     7261 tgggcggtcc ggccgagccg caggacggcg agggagcggc cctcgagtac gtactgctcg
     7321 ccggtgcgca gggacgggcg cgcgacgtcg ccgtcccagt cgcgcgtgtc gagcaccgga
     7381 tcccagctca cgcggctctc gtgggcgggg agcacgaacg gcaccggctc gtggtgggcg
     7441 ttgagcagga tcaggaaggt gtcgccgacg atgggctcgc ccaggtcgtc cacttcctcg
     7501 atggcgtcgc cggccagccg gaggccgagg cagcggccga agctgttctg ccactcctcg
     7561 tcggtcatct catggccgtc cgggcggaac caggcgaggt ctttcagcgc cgagccgtgg
     7621 atccggcgcc cctggaagaa catgcgccgg tggaagaccg ggttggcgtg cttgagccgg
     7681 atgaggcgcc gcgtgaactc gatcaggcgc tgcccggtct cggtgggcgg ccagtggaac
     7741 cacgacaggt cgctgtcctg gcaataggag ttgttgttgc cccgctgggt gcggcccatc
     7801 tcgtcgccgc ccaggagcat ggggatgccc tgggagagaa agagggtggc caggaagttc
     7861 cggagctgcc gctcgcgaat cgcgacgatc ctgggatcgc cggtgggccc ctcgacgccg
     7921 cagttccagg agttgttgtc gtcggtgccg tctcggttgt tctcgccgtt ggccaggttg
     7981 tgcttgtcgt tgtaggacac gaggtcggcg agggtgaagc cgtcgtgggc ggtgacgaag
     8041 ttgacgctcg cgtaggggcg tcgcccgccc atctcgtaga ggtcgctgga accggtgagg
     8101 cggtaggcca gctccgccac ctggccctcg tcacccttcc agtagcggcg gatggtgtcg
     8161 cggtacttcc cgttccactc cgtccacccc accgggaagt tgccgacctg gtagccgccc
     8221 tggcccagat cccagggctc ggcgatgagc ttgacctgcg agatcaccgg atcctggtgg
     8281 atgacgtcga agaaggcgga gagccggtcc acgtcgtgca gctcgcgcgc cagcgcggcc
     8341 gcgaggtcga agcggaagcc gtccacgtgc atctcgagca cccagtagcg caggctgtcc
     8401 atcaagagct ggatcgtgcg cgggtgtcgc atgttgagcg tgttgccgca cccggtgtag
     8461 tccacgtaga agcgcggatc ctcgggggac aggcggtagt aggccgcatt gtcgatgccg
     8521 cggaagcaga gcgtcggccc caggtggttg ccctccgcgg tgtggttgta caccacgtcg
     8581 aggatcacct cgatgcccgc ccggtgcagc cgtttcacca tgcgcttgaa ctcgtccacc
     8641 tgctggcccc gctgtcccga ggaggcgaaa cgcgaatcgg ggcagaagaa gccgatcgag
     8701 ttgtagcccc agtagttggt gaacccgcgg tcggcctggt acttctcggt gaccgagtgg
     8761 tggatgggca gcagctcgac cgcggtgacg ccgagctcgg tgaggtggcg gaccgccgcg
     8821 tcggaggcga ggcccgcgta ggtgccgcgc agctcgggcg gcacctcggg atggagcttg
     8881 gtgaagccct tgacgtggac ctcgtagatg acggtgcgcg accagcgggt gagcggcggc
     8941 cggtcgcctt cccagtcgaa cgtcccgtcc accaccacgc tcttgggcag gtacggtccg
     9001 ctgtcacgat cgtggggccg gatgtcgccc gcggggtcgc cgatgcggta gccgaacaag
     9061 gcgtcctgcc actcgagcgg gcccgcgatc gccttcgcgt aggggtcgag cagcgccttg
     9121 gccgggttga agcggtggcc cagggcgggc tcgtaggggc cgttcacccg gtacgcgtag
     9181 agcgtgccgg gacggactcc cggcacgtag gcgtgccaga cctgatcggt gcgctgctcg
     9241 atccggatct ggcggacctc cttgcccgga tcgcgctcgt cgaagaagca gagcgccacc
     9301 gcggtggcgt gctcgctgaa gagggcgaag ttgacgccgg cgccgtccca ggtggcgccg
     9361 agcggatagg gacggcccgg gcgggtgcgg ggagggtcgg ccatgggtgg ggccgcctca
     9421 ggccggggtc agccagaggg cgccgagcgg cggcagggtg agcaccaccg agtgcggctg
     9481 cccgtgccag gggacgggct cgctcgccac gccgcccgcg ttgcccacgt cgccgccgcc
     9541 gtagacggag gcgtccgagt tgacggtctc gcgatagaac ccggggcggg ggacgccgac
     9601 ccggtagccc cgccgcgcca ccggggtgaa gttgcacgcg cagaggagca gccgctcctg
     9661 gttccgggag aagcggcaga atgtcacgat gctttgctcc cagtcgctgc agtccatcca
     9721 ctggaagccc gccggctcga agtcgacttc gtggagcgaa ggctcgttcc ggtagacgtg
     9781 gttcaggtcc ttcaccagcc gctgcacccc gcggtggtag gggcccatgt cgagcagcga
     9841 ccagtccagg gcccggtcgt ggctccactc gtcggactgg ccgaactcgc cgcccatgaa
     9901 gaggagcttc ttgcccgggt aggcccacat gaacgcgtag agcaggcgga ggttcgcgaa
     9961 gcgctgccag tcgtcgccgg gcatcttgcg ggccagcgac cgcttgccgt agaccacctc
    10021 gtcgtgggag agcggcagca cgaagttctc cgtccacgcg tacaggaggc cgaaggtgag
    10081 ctggttgtgg tggtatttcc ggtgcaccgg ctcgtgctgc atgaactcga ggatgtcgtg
    10141 catccagccc atgttccact tgaagccgaa gccgagcccg cccaggtgga ccgggcgcga
    10201 gacctgaggc cacgcggtcg attcttcggc aatggtggag gcgcccggct ggccgccgta
    10261 gaccacctcg ttcagcttcc ggaggaaggc gagcgcctcc aggttctcgc ggccgccgta
    10321 ctcgttgggg atccactcgc ccgccttccg cgagtagtcc cggtagatca tcgaggcgac
    10381 cgcgtccac
//
LOCUS       DASUFA010000240        12461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_979935, whole genome shotgun sequence.
ACCESSION   DASUFA010000240 DASUFA010000000
VERSION     DASUFA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979935"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFX87_08970"
     CDS             <1..635
                     /locus_tag="VFX87_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995087.1"
                     /translation="AVKTDVSRLADVQALADRAFREFGAVHVVCNNAGVALWGGLEAV
                     THRDWEWAIGVNLWGVIHGVEAFVPRMVAQKQPGHVVNTASMAGLIASQGLGIYNTTK
                     YAVVGLSETLQKDLRGYDIGVSVLCPMGVHTQIRQSDRNRPAALRNAMTEREGGAVEL
                     IGRYLSPEHVAARVLRAVYANRLYVITHEEGLTPLKRRFERMAQAIEESK"
     gene            632..1558
                     /locus_tag="VFX87_08975"
     CDS             632..1558
                     /locus_tag="VFX87_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX4995088.1"
                     /translation="MKFSVIEGWGKLPDGWRYQEVAGAAVDKQDRVFCFTRSEHPVIV
                     FDRDGGFLRSWGEGVVRRAHGITIDADDMVWLTDDLHHTVRKFTPEGKLLLTIGDPDK
                     AAELYGGKPFNRPTHVAICPKSGYLFVSDGYGNSRVHKYAPDGKHVMSWGEPGTDPGQ
                     FNLPHNLVTDRDGLVYVADRENHRVQIFNGKGQYQGQWNNLHRPCGLFADRAGSGFFF
                     VGELGSGMPVTEKTPNLGPRVSVLDAKGQRVARFGGQFASEKPGEFIAPHGLVVDSHG
                     DVYVGEVSYTGYGQFLKPPREMRSFQKFERVN"
     gene            complement(1569..2081)
                     /locus_tag="VFX87_08980"
     CDS             complement(1569..2081)
                     /locus_tag="VFX87_08980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4995089.1"
                     /translation="MIHDPPLTPPFAARFASLDEVKLSGPRSIFHNCFGCGPRHDIGL
                     RVRTFSGDGEVLAPILIPRRFEGPPGSAHGGIVAAYLDEVLAGAAVNHTGRLYVTGEL
                     TVRYVKGTPIERPLLGRGRAVLDSGKYLDLEGTIEDWETGDVVARASGRFFPAPAAGS
                     VQRPRPGSVR"
     gene            2180..2368
                     /locus_tag="VFX87_08985"
     CDS             2180..2368
                     /locus_tag="VFX87_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX4995090.1"
                     /translation="MPVTVKVLTDGPLMVKGECEMQDGQGNAIAGKGGDAIFLCRCGS
                     SANKPFCDGAHKKIAFKG"
     gene            complement(2438..3598)
                     /locus_tag="VFX87_08990"
     CDS             complement(2438..3598)
                     /locus_tag="VFX87_08990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995091.1"
                     /translation="MSASSLSGTVLLCLVVFASTVCVGAFGPLLPEIARAQGLPDWQL
                     GILAGSFGFARMAADLPAGALATRHLATSLTLAPITLLAGMLLLSTAGPFPVLVLGRL
                     LTGLAHTLVMVGSLTAVLQDREGPRASMRLNTLEFAGMLGVLGGLALVGLLPERWPWN
                     LTLLTAAGPVVVSLAGTLVLTRTFRRHLPSAPPAATRAGAPGERRARAAALPPIVRLM
                     FALGVVMALAWSAVSQFVIPLRGTREFGLDRAGISGLLSLAQLVDLIVLLPVGWLADR
                     IGRLPVLTAVAGTLGFGLLAVGLGSLPLLVVGCALFGLGMAGWMLPLGVIREHTDVAR
                     LGWRTGVYRVGVDGAIFLGPLICGVLGEAHTPIFIALVGVAAFAIAVRLGRG"
     gene            complement(3612..4484)
                     /locus_tag="VFX87_08995"
     CDS             complement(3612..4484)
                     /locus_tag="VFX87_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX4995092.1"
                     /translation="MSAPTFEALRYEVADGVATVTLNRPEKLNALNTVMLEELLAVLE
                     LADADDGVRALIVTGAGRAFCAGADLSGGGKTFDRVARGRGEAPGAHRDGGGRVTLRI
                     FDMKKPLIAAINGPAVGFGVTMTLPMDIRIASTAARIGFVFARRGVVPEAASTWFLPR
                     LVGPSQAAEWVYTGRVFPAEEALAGRLVSRVVPPPLLLPVARELALEIAQNTSAISVA
                     LARQMMWRLLGADHPMEAHRLDSRCMDWTGRSADAYEGVTSFLEKRPPRFTQRPSTDM
                     PPFYPWWPERRFEP"
     gene            complement(4481..5917)
                     /locus_tag="VFX87_09000"
     CDS             complement(4481..5917)
                     /locus_tag="VFX87_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4995093.1"
                     /translation="MAEPGPPLATLLVADDDPAVRQSLERTLTREGYAVLLAPDGQAA
                     LERLREGGVDLVLSDLKMPGLTGLELLREAKAVAPDVDVILLTAFGTVEEAVRAMKDG
                     ATDFLTKPFQRAQLTKVVRQALERRDLIAQNRALQKRLDDLLRQGNMIGTSPAFRRLM
                     TLVQQVADSSATVLVQGESGTGKELVARAIHDRSSRRNGPFIAVNCAALPETLLESEL
                     FGYEKGAFTGAAGRKEGRFELADGGTLFLDEIADLSAVTQPKILRVLQEGEFERVGGT
                     KTIRVDVRIVTATNQDLATLVRDKRFREDLFYRLNVITIQVPPLREKHEDVPVLAQHF
                     LRVYAAKNNRQLDGFSDEALGCLEAYSWPGNVRELENVVERAVVLARGARVEAADLPD
                     AVRERPVMLMRGGPQAPGDGPGGAVVPEGVFQIRVGTPLAEVEARLLEETLRLNHGNK
                     TLTAKMLGIDPKTVFRKLKQGEAEDGAP"
     gene            complement(5910..7214)
                     /locus_tag="VFX87_09005"
     CDS             complement(5910..7214)
                     /locus_tag="VFX87_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4995094.1"
                     /translation="MAVTAPTRAPWAGLFQPLERDERLFAVLLAFVMVGGLATLGLVP
                     LRPRYRVDVYSLVLWFGLYKAGIFALVSANPRGTRRIFIAALCIDLVLVFLLLFLTGG
                     GDSPFVNLFFPLVAVNAYYFGRWAGLFLTLVAGLLYWAAASLALPAATWTAVLILMAL
                     VGLPAFALGHVADRERRARSEVERLNAELTGTLTRLRAAQENLVEAERMATVGRLSLK
                     VAHEVRNPIAAIGLNAELLGDMVQGRVNEDGTEATALVTAIRDQVGALDALTEEYLAF
                     ARFPRPQFEEDSVNDMVSAVAEFVRPLAARQGIAVKVVTDPTVPPMEIDRTLLRQAVL
                     NLVKNGMEAVPHGGTVTLTTACMGDTVEIAVSDSGAGISPEVGRRLFEQFFTTKPQGT
                     GLGLSISRQIVEEHGGKIRWHSAPGAGATFTMTVPVQKAAHG"
     gene            complement(7231..8691)
                     /locus_tag="VFX87_09010"
     CDS             complement(7231..8691)
                     /locus_tag="VFX87_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995095.1"
                     /translation="MPESIMPEPVETATTTAASRRGALLRRLFGSVRLAYLPVLVTYF
                     CYGASGITSIALLYFQKDALGITPAEAAAIAFWVALPWSTKMVAGVASDVHPIFGRRR
                     VSYLLLGALLSFAGYGWLATTASTKATYLAAMVIIAVGFMVQDVVADALSVELARNEE
                     EVKQIQTLGRMGLLAGTISVGYLSGWLAGALGPRPVFAIALLLPALVMAAAWLIPRET
                     ARAAAPGEEANPLGAGKSRLVVLVGLGYAALGVALEALSVPFGQEIILVVSGGLIVML
                     LRRVGISRAVAIAAFAIFCFRAVPSAGQGFNYWAIDRLGFDQEFLGILAQVGAVLSLV
                     GLIVFRKPITHRPVSYTLFWVTVAGAVLYLPTIGLFYGVNEWFGVSPRTFAFIDTTIS
                     APLAQLSMVPMLALIARTAPRGAEATMFAIMASLMNLALSASELGTRYLNTFFGVTQQ
                     DYSNLGRLLICVGVIGLFPLLALPLLRRVEAESGTP"
     gene            8766..9446
                     /locus_tag="VFX87_09015"
     CDS             8766..9446
                     /locus_tag="VFX87_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995096.1"
                     /translation="MTIAGKGVLAFWHDVAAGGDAEFDHWHLREHIPERVAVPGFLRC
                     RRYVTLGAPPKYFYFYETESLETLQSPAYLARLGDPTPWTKKTLPLVRNNKRTACRLV
                     ASAGAGLGGVIATLELGPRAGHEDELRSWLTATALPAATQHPGVVAGHLLEGDAGVST
                     AAKSDEKKLLATPDALVRWVVLVEGIDLSPIEAACRDLLGAEALARRGATDDVTLGFY
                     RLQLVFGK"
     gene            9468..10187
                     /locus_tag="VFX87_09020"
     CDS             9468..10187
                     /locus_tag="VFX87_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995097.1"
                     /translation="MIYAKAFFDLQVQFAERVTILSGLSMARVLLEYTNLFIRFGLGH
                     DFDPAHPAWQAYLAGLRDASDPRGWTYAFYLRRPDAMAAPSVVATFGCFAYARPTRER
                     IRLHFQNAETDGGSPLALDRVGARRAELAALFAHVKQTQDPPLQVRGVSWLYNLDAYR
                     RLFPTSYLATAPAAARRFQHMPLWGQFVDRDGAIKEKPTRQFLERLERQSSLDALEQC
                     FPFQPLAVDAPVQTFYEFYGL"
     gene            10426..11370
                     /locus_tag="VFX87_09025"
     CDS             10426..11370
                     /locus_tag="VFX87_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995098.1"
                     /translation="MVRFVLSRLGQTLAVLFTVSVIIFALMRMIPGDPVLMRLGDDFS
                     QEAYENLRRDLGFDRSVVVQYLLWIRNVLAGDLGDSFINHERVSTLVLDAFVPTLTLV
                     TASLVVGILIAVPSGLIAAMWKDSAWDYGSMGFAIFVYSMPAFWKGILLIWFFSVYLG
                     WFPAMGYVHPWTDLSQGLWRLVLPAVTLGTFFSGLVARIIRSSLLEVLDQDYVKAARA
                     RGVRQAALVYKHALANALIPVVTVIGLQFGTLLGGAVLTETVFAIPGMGRLSVSAILN
                     RDYLVVQGAILVGVFAVVVVNLLVDLLYAFLNPRIRVA"
     gene            11370..12308
                     /locus_tag="VFX87_09030"
     CDS             11370..12308
                     /locus_tag="VFX87_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995099.1"
                     /translation="MAQPLGAGDRPFDAEGLGVPPAPVVASPVTPGARAGIRRWWRRF
                     VRNPGAVFGLAVFLAIVAMAILAPVLAPYDPIAQGVGTPLERPSAAHWAGTDSFGRDI
                     MSRIIYGARVALVVGLVAVLLAMVIGVTLGLISGYYGGIVDTVIMRIMDGLFAFPIII
                     LAIAMMAVMGFGVRNVIIAVAVGFIAPFARVTRGDVLAVKEEPYIEAARLAGVGSLAI
                     IFQHVLPNVLAPIIVQGALRVSGAIITESGLSFLGLGPPPPTPAWGRMIAEGQTFIVM
                     APHVSTIPGIALMLAVVALNLLGDGLRDTLDPRMRR"
     gene            12392..>12461
                     /locus_tag="VFX87_09035"
     CDS             12392..>12461
                     /locus_tag="VFX87_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4995100.1"
                     /translation="MSFQIRRGEIVGLVGESGAGKSL"
BASE COUNT         1870 a         4386 c         4435 g         1770 t
ORIGIN      
        1 tggccgtgaa gaccgacgtg agccggctcg ccgacgtgca ggcgctggcc gatcgggcct
       61 tccgagagtt cggcgcggtg cacgtggttt gcaacaacgc gggcgtcgcc ctctggggcg
      121 ggctcgaggc cgtcacccac agggactggg agtgggcgat tggcgtcaac ctctggggcg
      181 tcatccacgg agtggaggcc ttcgtgccgc gcatggtggc ccagaaacaa ccagggcacg
      241 tggtgaacac cgcgtccatg gccgggctca tcgcctcgca ggggctcggc atctacaaca
      301 cgaccaagta cgcggtggtc ggcctctccg agacgctcca gaaggacctg cgcggctacg
      361 acatcggcgt ctcggtgctc tgtccgatgg gcgtgcacac ccagatccgg cagagcgacc
      421 ggaaccggcc cgccgcgctc cgcaacgcga tgacggagcg cgagggcggg gcggtggagc
      481 tgatcggccg ctatctctca ccggagcacg tggcggctcg cgtgctgcgc gccgtctacg
      541 ccaaccgcct ctacgtgatc acgcacgagg aagggctgac tccgctcaag cggcgcttcg
      601 agcgcatggc ccaggccatc gaggagtcga aatgaaattc tcggtcatcg agggctgggg
      661 caagctgccc gacggctggc ggtaccagga agtggcgggc gcggcggtgg acaagcagga
      721 ccgggtcttc tgcttcacgc ggagcgagca cccggtcatc gtgttcgacc gcgacggcgg
      781 cttcctccgc tcgtggggcg agggcgtggt ccgccgcgcc cacggcatca ccatcgacgc
      841 cgacgacatg gtgtggctca ccgacgatct ccaccatacc gtccgcaagt tcacgccgga
      901 gggcaagctg ttgctgacca tcggcgaccc ggacaaggcc gcggagctct acggcggcaa
      961 gccgttcaac cgacccaccc acgtggcgat ctgcccgaag agcggctacc tcttcgtctc
     1021 cgacggctac ggcaactcgc gggtgcacaa gtacgcgccg gacggcaagc acgtcatgtc
     1081 gtggggcgag ccgggcaccg atcccgggca gttcaacctg ccccacaatc tcgtcaccga
     1141 ccgcgacggc ctcgtctacg tggccgaccg cgagaaccac cgcgtgcaga tcttcaacgg
     1201 caagggccag taccagggcc agtggaacaa tctccaccgc ccctgcgggc tcttcgcgga
     1261 ccgcgccggg agcgggttct tcttcgtggg cgagctcggc tccggcatgc cggtcaccga
     1321 gaagacgccg aacctgggcc cgcgggtcag cgtgctcgac gccaagggcc agcgggtggc
     1381 gcgcttcggc ggccagttcg cgagcgagaa gccgggagag ttcatcgcac cccacgggct
     1441 cgtggtggac tcgcacgggg acgtctacgt gggcgaggtc tcctacaccg gctacgggca
     1501 attcctcaag ccgccgcggg agatgcggtc cttccagaag ttcgagcgcg tcaactaggc
     1561 gccgctgttc atcgaacaga ccccggccgc gggcgctgaa cagaaccggc ggcgggcgcc
     1621 ggaaagaacc ggccgctggc cctcgccacg acgtcgccgg tctcccagtc ctcgatggtg
     1681 ccttccagat cgaggtactt gccggagtcg aggaccgcgc gaccccgccc gagcagcgga
     1741 cgctcgatgg gcgtgccctt cacgtagcgc accgtcagct ctccggtcac gtagaggcgc
     1801 ccggtgtggt tcaccgcggc gcccgccagc acctcgtcga gatacgccgc cacgatgccg
     1861 ccgtgggccg agccgggcgg gccctcgaag cggcgcggaa tcaggatggg cgcgagcact
     1921 tccccgtccc cgctgaacgt ccggacgcgc aggccgatgt cgtgccgggg cccgcagccg
     1981 aagcagttgt gaaagatcga gcgcggcccg ctgagcttca cctcgtcgag ggaggcgaac
     2041 cgcgcggcga agggcggcgt caggggcggg tcgtggatca ccggaagtag gaccggagca
     2101 gcgccggagg ctcggccgcc atgtcgacct ggagtatact cggcccgcgg tagctcgatc
     2161 atgacgatga ggtgaaacga tgcccgtgac cgtgaaggtt ctgaccgacg gccccctgat
     2221 ggtgaagggc gagtgtgaga tgcaggacgg ccaaggcaac gccatcgccg gcaagggcgg
     2281 tgacgccatc ttcctctgcc gctgcggcag ctcggccaac aagccgttct gcgacggcgc
     2341 ccacaagaag atcgcgttca aaggctgacg cagtcacacc ctctccccct ctccccgctg
     2401 gggagagggt agggaggggt ggtcgtgagg ggccagctta cccccgcccc agcctcaccg
     2461 cgatcgcgaa ggcggccact cccaccagcg cgatgaagat cggggtgtgg gcttcgccga
     2521 gcacgccgca gatcagcggg ccgaggaaga tcgccccgtc cacgccgacg cggtagacac
     2581 cggtccgcca gccgagccgg gcgacgtccg tgtgctcacg gatgaccccg agcggcagca
     2641 tccagcccgc catcccgagg ccgaacagcg cgcagccgac gacgagcagc ggcagcgagc
     2701 cgagtcccac cgcgaggagc ccgaagccga gggtgcccgc gaccgcggtc agcacgggca
     2761 gccggccgat gcggtcggcc agccagccca ccggcaggag cacgatcagg tccacgagct
     2821 gggccagcga gagcaggccg gagatgccgg cgcggtcgag cccgaactcc cgcgtcccgc
     2881 gcagcgggat cacgaactgg ctcaccgcgg accaggcgag cgccatcacc acgcccagcg
     2941 cgaacatgag ccgcacgatc ggcggcagcg ccgccgcccg cgcccgcctc tcgcccgggg
     3001 cccccgcccg ggtggcggcg ggcggcgcgg acggaaggtg gcgccggaat gtgcgggtca
     3061 gcacgagcgt gccggccagc gacaccacca ccggtccggc ggcggtcagc agggtcaggt
     3121 tccacggcca gcgctccggg aggagtccga ccagggcgag cccgccgagg accccgagca
     3181 tgcccgcgaa ctcgagcgtg ttcagccgca tggaggcgcg cgggccctct cgatcctgca
     3241 gcacggcggt caagctgccg accatcacga gcgtgtgcgc gaggccggtc aggaggcggc
     3301 cgagcacgag caccgggaac ggccccgcgg tggacagcag cagcatcccg gcgagcagcg
     3361 tgatgggagc cagcgtgagc gaggtggcga ggtgccgggt ggcgagcgcg cccgccggca
     3421 gatccgccgc catccgcgcg aaaccgaagg agccggcgag gatgccgagc tgccagtccg
     3481 gcagaccctg cgcccgcgcg atctcgggca gcagcgggcc gaacgcgccg acgcacaccg
     3541 tgctggcgaa gacgaccagg cacagcagca ccgtgccgga cagcgaggac gcgctcaccg
     3601 ccgagggcgg ctcagggctc gaagcggcgc tcgggccacc agggatagaa gggcggcatg
     3661 tcggtgctgg ggcgctgcgt gaagcgcggc gggcgcttct ccaggaacga cgtcacgccc
     3721 tcgtaggcgt cggccgagcg gcccgtccag tccatgcagc gcgagtcgag gcggtgcgcc
     3781 tccatcgggt ggtcggcgcc cagcagccgc cacatcatct gccgcgcgag cgccaccgag
     3841 atcgccgatg tattctgcgc gatctcgagt gcgagctcgc gggccaccgg caggaggagc
     3901 ggaggcggga ccacccggct cacgagccgg cccgcgagcg cttcctccgc cgggaagacc
     3961 cgcccggtgt agacccactc cgcggcctgg ctcgggccca ccaggcgcgg cagaaaccag
     4021 gtgctggccg cctccggcac gacgccgcgg cgggcgaaca cgaagccgat gcgcgccgcg
     4081 gtcgaggcga tgcgaatgtc catcggcagc gtcatggtga cgccgaatcc caccgcgggg
     4141 ccgttgatgg cggcgatcag cggcttcttc atgtcgaaga tgcggagggt cacgcgcccg
     4201 cccccgtccc ggtgcgcccc gggcgcctcg ccccggcccc gggcgacgcg gtcgaaggtc
     4261 ttgccgcccc cggacagatc cgctcccgcg cagaacgcgc ggcccgcccc ggtgacgatc
     4321 agcgcgcgca ccccgtcgtc ggcatcggcc agctcgagga ccgcgagcag ctcttccagc
     4381 atgacggtgt tgagcgcgtt gagcttctcc ggccggttca gggtgacggt ggcgacaccg
     4441 tccgccacct cgtagcggag cgcctcgaag gtcggggcgc tcacggcgcc ccgtcctcgg
     4501 cctcaccctg cttgagcttg cggaagacgg tcttgggatc gatgcccagc atcttggcgg
     4561 tgagcgtctt gttgccgtgg ttgagccgga gcgtctcctc gaggagccgc gcctcgacct
     4621 cggccagcgg cgtgcccacg cggatctgga agacgccctc gggcaccacg gccccgccgg
     4681 gcccgtcacc gggcgcttgc ggcccgccgc gcatcagcat gaccgggcgc tcccgtaccg
     4741 cgtcgggcag gtcggccgcc tccacgcggg cgccgcgggc cagcaccacc gcgcgctcga
     4801 ccacgttctc gagctcgcgc acgttgcccg gccacgagta ggcctcgagg cagccgagcg
     4861 cctcgtcgct gaagccgtcg agctgccggt tgttcttggc cgcgtacacg cgcaggaagt
     4921 gctgggcgag caccggcacg tcctcgtgct tctcccggag cggcgggacc tggatcgtga
     4981 tgacgttgag gcggtagaag aggtcctccc ggaaccgctt gtcgcgcacg agggtggcca
     5041 ggtcctggtt ggtagcggtc acgatgcgca cgtccacccg gatcgtcttg gtgccgccca
     5101 cccgctcgaa ctcgccctcc tgcagcacgc gcaggatctt gggctgggtc accgccgaga
     5161 gatcggcgat ctcgtcgagg aacagggtcc cgccgtcggc cagctcgaag cggccctcct
     5221 tgcgcccggc cgccccggtg aacgccccct tctcgtagcc gaacagctcg gattcgagca
     5281 gcgtctcggg cagggccgcg cagttgaccg cgatgaacgg cccgttgcgg cggctcgaac
     5341 gatcgtggat ggcgcgcgcc accagctcct tgccggtgcc gctctcgccc tgcacgagca
     5401 cggtggccga gctgtccgcg acctgctgca cgagcgtcat cagccggcgg aacgccgggc
     5461 tggtcccgat catgttgccc tggcgcagca gatcgtcgag gcgcttctgg agcgcccggt
     5521 tctgggcgat gaggtcgcgg cgctcgaggg cctgccgcac caccttggtg agctgggcgc
     5581 gctggaacgg cttggtgagg aagtcggtgg ccccgtcctt catggcccgg accgcctcct
     5641 ccacggtgcc gaaggcggtg agcaggatca cgtccacgtc gggcgcgacc gccttggcct
     5701 cccgcagcag ctcgaggccg gtgaggcccg gcatcttgag gtcggagagc acgagatcca
     5761 ccccgccctc ccgcaaccgc tccagcgcgg cctgaccgtc gggggcgagc aggaccgcgt
     5821 agccttcacg cgtcagcgtg cgctcgagac tctggcggac ggcgggatcg tcgtcggcca
     5881 ccaggagcgt cgcgagcggc gggccgggct cagccatggg ccgctttctg gacgggcacc
     5941 gtcatggtga aggtcgcgcc cgcgccgggc gcgctgtgcc agcggatctt gccgccgtgc
     6001 tcctcgacga tctggcgcga gatggagagg ccgaggcccg tgccctgcgg cttggtggtg
     6061 aagaactgct cgaacagccg ccggccgacc tcgggcgaga tgccggcgcc gctgtccgag
     6121 acggcgatct ccaccgtgtc acccatgcag gcggtggtga gggtcacggt gccgccgtgg
     6181 gggaccgcct ccatcccgtt cttcaccagg ttcagcaccg cctggcggag cagggtgcgg
     6241 tcgatctcca tcggaggcac ggtggggtcg gtcaccacct tcaccgcgat gccctggcgc
     6301 gcagcgagcg ggcggacgaa ctccgcgacc gcgctgacca tgtcgttgac ggagtcctcc
     6361 tcgaactggg ggcgcgggaa ccgcgcgaag gccaggtact cctcggtgag ggcgtcgagg
     6421 gcccccacct ggtcgcggat cgcggtgacg agcgcggtcg cttcggtgcc gtcctcgttc
     6481 acccgcccct gcaccatgtc gcccagcagc tccgcgttca gcccgatggc cgcgatgggg
     6541 ttgcgcacct cgtgcgcgac cttgagcgac agccgcccca ccgtggccat ccgctcggcc
     6601 tcgaccaggt tttcctgggc ggcccggagg cgggtgagcg tcccggtcag ctcggcgttg
     6661 agccgctcca cttccgagcg cgcgcggcgc tcgcgatcgg ccacgtgacc gagcgcgaaa
     6721 gccgggagcc cgaccagggc catcaggatc agcaccgcgg tccacgtcgc ggcgggaagc
     6781 gccaggctgg cggcggccca gtagagcagc ccggccacga gcgtgaggaa cagaccggcc
     6841 cagcggccga agtagtaggc attgaccgcg accagcggga agaacaggtt gacgaacggg
     6901 ctgtcgccgc ccccggtgag gaagagcagg aggaagacga gcacgagatc gatgcagagg
     6961 gcggcgatga agatccgccg ggtcccgcgc gggttggcgc tgaccagcgc gaagatgccc
     7021 gccttgtaga ggccgaacca gaggacgagg gagtagacgt ccacccggta ccgcgggcgc
     7081 agcgggacga ggccgagggt ggcgaggccg cccaccatca cgaaggcgag gagcaccgcg
     7141 aacagccgct cgtcccgctc gagcggctgg aacagcccgg cccagggcgc gcgagtgggg
     7201 gcggtcaccg ccatgacggg ggacacacta tcacggcgtc ccggactccg cctccacgcg
     7261 ccgcaggagc ggcagggcga gcagcgggaa caggccgatc acccccacgc agatgagcag
     7321 gcggcccagg ttggagtagt cctgctgggt gacgccgaag aaggtgttga ggtagcgcgt
     7381 gccgagctcg ctggccgaca gcgcgaggtt catgagggag gccatgatcg cgaacatggt
     7441 ggcctccgcc ccgcgcggcg cggtgcgcgc gatgagcgcg agcatcggca ccatgctgag
     7501 ctgggccagc ggcgccgaga tcgtggtgtc gatgaacgcg aacgtgcgcg ggctcacccc
     7561 gaaccactcg ttgacgccgt agaacagccc gatcgtcggc aggtagagca ccgcgcccgc
     7621 gaccgtcacc cagaagaggg tgtagctcac ggggcgatgc gtgatcggct tgcggaagac
     7681 gatgagaccg accaggctca gcaccgcgcc cacctgcgcg agaatgccca ggaactcctg
     7741 atcgaagccg agccgatcga tcgcccagta gttgaacccc tgccccgccg acgggacggc
     7801 ccggaagcag aagatggcga aggcggcgat cgccaccgcg cgggagatcc cgacccgccg
     7861 caggagcatc acgatgaggc cgcccgagac gaccaggatg atctcctggc cgaacggcac
     7921 cgacagcgcc tccagcgcga cgccgagcgc cgcgtagccg aggcccacga gcaccacgag
     7981 gcgcgacttg cccgcgccca ggggattcgc ctcctccccg ggcgccgccg cgcgggcggt
     8041 ctcgcgcggg atcagccagg ccgccgccat caccagcgcc gggagcagga gggcgatcgc
     8101 gaagacgggg cgcgggccga gcgcgcccgc gagccacccg ctcaagtagc ccacgctgat
     8161 cgtgcccgca agcaggccca tccggccgag cgtctggatc tgcttcacct cctcctcgtt
     8221 cctggcgagc tccacgctca gcgcgtcggc caccacgtcc tggaccatga agcccaccgc
     8281 gatgatcacc atcgcggcca ggtaggtggc cttggtcgag gcggtggtgg ccagccaccc
     8341 gtagcccgcg aaggacagca gggcgcccag cagcaggtag gagacccggc ggcggccgaa
     8401 gatcgggtgg acgtcggagg ccacgccggc caccatcttg gtgctccacg gcagggcaac
     8461 ccagaaggcg atcgccgcgg cctcggcggg cgtgatgccg agcgcgtcct tctggaaata
     8521 gagcagcgcg atgctcgtga tcccgctcgc cccatagcag aagtaggtca cgagcaccgg
     8581 cagataggcc aggcgcacgc tcccgaagag gcggcgcagg agggcgccgc ggcgggacgc
     8641 ggcggtggtg gtcgcggtct cgacaggttc cggcatgatg gattcgggca tggcgtccag
     8701 tttagcgcac tgcaaccgtg cgggactctt gacgcgaagc ccccggcccc gttaccgtcg
     8761 gcgccatgac catcgcgggc aagggagtcc tggcgttctg gcacgacgtg gcggcgggcg
     8821 gtgacgcgga attcgatcat tggcatctgc gcgagcacat tccggagcgc gtggccgtgc
     8881 cgggcttcct ccgctgccgg cgctacgtca ccctgggtgc gccgccgaag tacttctatt
     8941 tctacgagac cgagtcgctc gagacgctgc agagcccggc ctacctggcc cgcctgggcg
     9001 accccacgcc gtggacgaag aagacgctgc cgctggtgcg caacaacaag cgcaccgcct
     9061 gccgcctcgt ggcgagcgcg ggcgcgggcc tgggcggcgt gatcgcgacc ctcgagctcg
     9121 ggccgcgcgc cgggcacgag gacgagctcc ggtcgtggct caccgccacc gcgctccccg
     9181 ccgccaccca gcacccgggc gtcgtggccg gccatctcct ggaaggcgac gccggcgtga
     9241 gcaccgccgc caagagcgac gagaagaagc tgctcgcgac ccccgacgcg ctggtgcgct
     9301 gggtcgtgct cgtcgagggc atcgacctct ccccgatcga ggccgcctgc cgcgacctgc
     9361 tcggcgccga ggccctggcc cgccgcggcg ccaccgacga cgtcacgctc gggttctacc
     9421 gcctgcagct cgtcttcggg aagtaggcgc gcgggcaagt ccgtcgtgtg atctacgcga
     9481 aggcgttctt cgatcttcag gttcagtttg ccgagcgggt gacgatactc tcgggactct
     9541 ccatggcccg cgtgctgctg gagtacacca atctctttat ccggttcggc ctgggccatg
     9601 atttcgatcc cgcgcatccg gcctggcagg cgtacctggc cggcctccgg gacgcgagtg
     9661 atccgcgcgg gtggacgtac gccttctacc tgaggcgacc cgatgcgatg gccgcgccgt
     9721 cggtcgtggc gaccttcggc tgcttcgcct acgcgcggcc gacccgcgag cggatccgtc
     9781 tccacttcca gaatgccgag acggatgggg gctccccgct ggccctcgac cgcgtcgggg
     9841 cgcgacgggc cgagctggcc gccctcttcg cgcatgtgaa gcagacccag gacccgccgc
     9901 tccaggtgcg cggggtgtcg tggctctaca acctcgacgc gtatcgtcgg ctgttcccga
     9961 cctcgtacct ggccaccgcg cccgcggccg cccggcgatt tcagcacatg cccctctggg
    10021 ggcaattcgt ggaccgagat ggggcgatca aggagaagcc gacgcgtcag ttcctggagc
    10081 ggctcgagcg ccagtcgagt ctggacgccc tggagcagtg ctttccgttc cagccgctcg
    10141 cggtggacgc cccggtgcag accttctacg agttctacgg gctctagccc gcgggcctcc
    10201 gtcagggtag cgccggccgg cgcttgccac tcggtggcgt gctggtacgc gtggccgaga
    10261 cgcagcacgg tggccgcgtg aaggcggtca gctcctgcgg acccgccttg cgggggtggg
    10321 gggcgcgcag tatactcgct tcccaattgc gggccgatgt cgtcccgagg gacgtgtgtc
    10381 ggcacgcaca tccgctcggg ttcggcagga tcggccggag agaccatggt ccgattcgtc
    10441 ctctctcgtc tcgggcagac cctggccgtg ctcttcacgg tctcggtgat catctttgcc
    10501 ctgatgcgga tgatcccggg cgaccccgtc ctgatgcgcc tcggcgacga tttcagccag
    10561 gaggcctacg agaacctccg ccgagacctc ggcttcgacc gctccgtcgt cgtgcagtat
    10621 ctcctctgga tccgcaacgt cctcgcgggc gatctcggcg actccttcat caatcacgag
    10681 cgggtctcca cgctggtgct cgacgcgttc gtccccaccc tgaccctggt gacggccagc
    10741 ctggtcgtgg gcatcctgat cgcggtgccg tcggggctca tcgcggcgat gtggaaggac
    10801 tcggcgtggg actacgggtc gatgggcttc gcgatcttcg tctactcgat gcccgcattc
    10861 tggaagggca ttctcctgat ctggttcttc agcgtgtacc tcggctggtt tcccgcgatg
    10921 ggctacgttc acccgtggac ggacctctcc caggggctct ggcggctcgt gctgcccgcg
    10981 gtgacgctcg gcaccttctt ctccgggctg gtggcccgga tcatccgttc gagcctgctc
    11041 gaggtcctcg accaggacta cgtcaaggcc gcgcgggccc gcggtgtccg ccaggccgcc
    11101 ctggtctaca agcacgcgct ggccaacgcc ctcatcccgg tggtgacggt gatcgggcta
    11161 cagttcggca ccctgctggg cggcgcggtc ctgaccgaga ccgtgttcgc gatcccgggc
    11221 atgggccggc tctccgtgag cgcgatcttg aaccgcgact acctggtcgt gcagggcgcg
    11281 atcctggtcg gggtcttcgc ggtggtggtg gtcaatcttc ttgtggatct cctgtacgcg
    11341 ttcctcaacc cgcggatccg ggtggcctag tggcgcagcc cctcggcgcc ggcgatcgcc
    11401 cgttcgacgc cgagggcctg ggggttcccc ccgcccccgt ggtcgcgtca cccgtgacgc
    11461 cgggagcgcg ggccggcatc cgccgctggt ggcggcggtt cgtgcggaac cccggcgcgg
    11521 tgttcgggct cgcggtgttc ctggcgatcg tcgcgatggc gatcctggcg cccgtcctcg
    11581 cgccctacga tccgatcgcc cagggcgtgg gcacgccgct cgagcggccg tccgcggccc
    11641 actgggcggg caccgacagc ttcggccgcg acatcatgag ccggatcatc tacggggcgc
    11701 gggtggccct ggtggtcggc ctggtcgcgg tgctgctagc catggtcatc ggggtgacgc
    11761 tcggcctgat ctcggggtac tacggcggga tcgtggacac cgtgatcatg cgcatcatgg
    11821 acgggctctt cgcgttcccg atcatcatcc tcgccatcgc gatgatggcg gtgatggggt
    11881 tcggggtgcg caacgtgatc atcgcggtcg cggtcggctt catcgccccg ttcgcgcgtg
    11941 tgacccgtgg cgacgtgctc gcggtaaagg aggagcccta catcgaggcg gcgcgcctgg
    12001 ccggagtagg cagcctcgcg atcatctttc agcatgtgct gcccaacgtg ctggccccga
    12061 tcatcgtcca gggcgcgctc cgcgtctcgg gcgcgatcat caccgagtcc gggctgtcgt
    12121 tcctgggcct cggcccgccg ccgcccacgc cggcctgggg tcgcatgatc gccgagggcc
    12181 agaccttcat cgtgatggcg ccccacgtct cgaccattcc cggcatcgcg ctgatgctgg
    12241 ccgtcgtcgc gctcaacctc ctgggcgacg ggctgcgcga caccctcgac ccacggatgc
    12301 gccggtgacc gattccgggc ccgttctcga cgtccgaggc ctcaccgtcg agttcagctc
    12361 ggaggacggc ccgctgcgcg cggtggacga ggtgagcttc cagatccggc gtggagagat
    12421 cgtcgggctg gtgggcgagt ccggcgcggg caagtcgctg a
//
LOCUS       DASUFA010000241         6850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_383640, whole genome shotgun sequence.
ACCESSION   DASUFA010000241 DASUFA010000000
VERSION     DASUFA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383640"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="VFX87_09040"
     CDS             complement(<1..1599)
                     /locus_tag="VFX87_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995101.1"
                     /translation="MAYDLLIKNGWVVDGSGLPRYRGDVGVKAGKIVAIGRLRESAKE
                     VIDAEGRVVAPGFVDGHTHMDAQIFWDPLGTSSCWHGITSVVMGNCGFTLAPCAKEDR
                     HLVIRNLERAEDIAGEAMEAGIDWRWTTFPEFLDTLESLPKGINYSGYIGHSALRTYV
                     MGERAFEQAASEADLAAMERELRNALMAGAIGFTTSRSPSHETPDAKPVASRMATWGE
                     VQRLVGAMGELNAGIFELAGEGVDRKVDHPDLPDYHRRLKALAVETGRPITFGLFTRK
                     DAPDMWRHYVKLIEETNAAGGRMFAQVHSRALSVVLSFKTQLPFDRLPVWKELRALPL
                     GEQQTRLRDPELRKKLVAAARERDNRKAIGTEARQAVYDWLFVMDSVDPPHRSVAEIA
                     RERGVDPVEAMIDLALEKDMDRFFFQPIANENQELAIELIKHPQAVVTFSDSGAHVSQ
                     LMDSSLQTYLLSHWVRTKQALTLEQAVRMLSFVPASYWGFTDRGLIREGMAADFVVFD
                     PDTIAAELPEVVRDLPAGAKRLIQR"
     gene            1698..2714
                     /locus_tag="VFX87_09045"
     CDS             1698..2714
                     /locus_tag="VFX87_09045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995102.1"
                     /translation="MDLGIFDHLDRRDIPVADFYEHRLRLLEKYDAAGFYSYHLAEHH
                     ATPLGLAPMPGIFLAAATQRTRRIRLGPCVYCLPLYNPLRLIEEVCMLDHLSRGRFDF
                     GVGRGIVPYEMAFFDLHHLETEDIYRETLEIVLQGLTSDVLDHRGSKYIYRKVPMILK
                     PLQKPHPPLWYGLGHTAGAEWAAANRVNVITNLGAEMSAPLFARYREVWQRKHGDAAL
                     PRLGMGRHVVVAPTEDAAMALGRASYAVWYANLTKLWRDFGAIPFRFAPDFDEARRRG
                     VAIAGTPAQVRAEFERQVAASACTYMTCRLMFGEMTEAEAAANVDLFVAEVMPHVTAL
                     AVPV"
     gene            2719..3486
                     /locus_tag="VFX87_09050"
     CDS             2719..3486
                     /locus_tag="VFX87_09050"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4995103.1"
                     /translation="MRLKGRTAFVTGASRNIGRAIALAFAAEGADLVLNTRSNRDELE
                     AVAEECRKRGVRAAAVLGDIGDPDAVEAMVRNGLAVFGAIDVLVCNAAIRPHKSVTET
                     SLEDWHRVFAVNLHSAFYLARAVVPGMKERGRGSIIALGGQSSLTGRPGTAAVTAAKT
                     GLLGLVRALAAELGPSGIRVNMVVPGTIDTERRYAEWYPEFRQAPPGGAEQLKRIPLG
                     RLGRPEEIAEACLFLASDASAYVTGDTIRVMGGSFIG"
     gene            3503..3838
                     /locus_tag="VFX87_09055"
     CDS             3503..3838
                     /locus_tag="VFX87_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HEX4995104.1"
                     /translation="MAAQAMVLEFGMGVDVHGDEGTKAACRAVSDAIRHSSLPLFTEV
                     RAKGGRMLVDVTVGVPDPGKVDVERVKKELPHGEVTVRPVQGGLRVPNADTIIACAAI
                     TVSVEYPGR"
     gene            3879..4364
                     /locus_tag="VFX87_09060"
     CDS             3879..4364
                     /locus_tag="VFX87_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4995105.1"
                     /translation="MSGIGLKRLQHLVLWVSDVDRSVRFYCDVLGFEVKTRYPNAAFL
                     RIAGSSEDHHLGLFEQPGVTGPDEGAVRMYHSAWEVGELTDLVRARRRLIEAHSLVGS
                     SDHGVSLSLYAKDPDGLEFEVFWTVPGGRPVGTRALDLEGELARRGIPMPQEIDVAPS
                     T"
     gene            4361..>6850
                     /locus_tag="VFX87_09065"
     CDS             4361..>6850
                     /locus_tag="VFX87_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4995106.1"
                     /translation="MTCSGCGFEAPADFAFCPKCGGRLAATPAAPPPPSPPAAPDSDR
                     RPVTVLFADLAGFTALSEGLDPEDVRAIQSDLFREMSESIERYEGFVEKFVGDAVMAV
                     FGAPRAHEDDPERGLRAALLMRDRMGTLNRRWERRVGRPLALHIGINTGPVVAGRIGT
                     SADAAYAVTGDTVNTASRLQSAAPPGEILISDATYQLTHHAFAFAPREAIALKGKSEP
                     AGIHRLLGPLAAPRSARGLEGLGLAAPLVGRERELRRMDAAFDDMRAGRAQVLSLIGE
                     PGAGKSRLQREFFARLEAAGRLEGVTIRRATCSALGEQTYGAAAALLRDAYGVEHGDS
                     VEVARGKLAAGLETLGVDAEDRAAMVAALARVFGLEQDDARIRHLEPEQLKRQIFMAT
                     YALVERRLAAGPLVLVVEDLHWADAASIELLGVVADRLADRPLLVLLMYRPTLEPDAL
                     GTSRTPHTAIEVMPLSRSDSEDLLAAWFGDSTHLFPEHLRVLILERAGGNPLYLEEVV
                     RALLAAGVLVRDGQVWRCTAEAATAQVPATLHGLLLARLDRLDAVERRVIQEAAVIGP
                     HFPVSLLKAASAEPAAVDAALDALAGADLVTPGPDHQFLHGLLQEIVYQNILVARRTE
                     LHTRVATALEAQGVGGSESLERLVALGHHWALGADKGRGARYLVSAGDWARGMYANAD
                     AILHYQRALDALAQCDAPAAATQIETERLVARERMADLLGPTGRRAAALGEYEIVEAG
                     YRAAGDAAAQARILRKMGGLHWDAGARARALQCFEAGLALLGDHREHIEQAHLYQEMG
                     RLAFRSGESQRAVEWAEQARLHAERLAADPAL"
BASE COUNT          943 a         2395 c         2511 g         1001 t
ORIGIN      
        1 gcgctggatc agccgcttgg cccccgcggg caggtcccgc accacctcgg gcagctcggc
       61 cgcgatggtg tcgggatcga agaccacgaa gtcggcggcc atgccctcgc ggatcaggcc
      121 gcgatccgtg aagccccagt agctggcggg tacgaacgag agcatgcgca ccgcctgctc
      181 gagcgtgagc gcctgcttgg tgcgcaccca gtggctcagc agataggtct ggagcgagga
      241 gtccatgagc tgggagacgt gggcgcccga gtcggagaag gtgaccaccg cctgcggatg
      301 cttgatcagc tcgatcgcca gctcctggtt ctcgttggcg atcggctgga agaagaaacg
      361 gtccatgtcc ttctcgagcg ccaggtcgat catcgcctcg accgggtcca ccccgcgctc
      421 gcgcgcgatc tcggccaccg accggtgcgg cgggtccacc gagtccatca cgaagagcca
      481 atcgtagacg gcctgccggg cctcggtgcc gatggccttc cggttgtcgc gctcgcgcgc
      541 ggccgcgacc agcttcttcc gcagctcggg gtcgcgcagc cgcgtctgtt gctcgccgag
      601 cggcagcgcg cggagctcct tccacaccgg cagccggtcg aacgggagct gagtcttgaa
      661 cgagagcacc acgctgagcg cccggctgtg cacctgggcg aacatgcgcc cgcccgcggc
      721 gttggtctcc tcgatcagct tcacgtagtg gcgccacatg tcgggcgcgt ccttgcgggt
      781 gaagaggccg aaggtgatcg ggcggccggt ctccacggcc agggccttga gccggcggtg
      841 gtagtcgggc agatccgggt ggtcgacctt gcggtccacg ccctccccgg ccagctcgaa
      901 gatgcccgcg ttcagctcgc ccatcgcgcc gacgagccgc tgcacctcac cccaggtggc
      961 catgcggctc gcgaccggct tggcgtcggg cgtctcgtgg ctgggcgacc gtgaggtcgt
     1021 gaagccgatg gcgccggcca tgagcgcgtt gcgcagctcg cgctccatcg cggccaggtc
     1081 ggcctcgctc gcggcctgct cgaaggcgcg ctcgcccatc acgtaggtgc ggagtgcgga
     1141 gtggccgatg tagccggagt agttgatgcc cttgggcagg gactccagcg tgtcgaggaa
     1201 ctcggggaag gtggtccagc gccagtcgat gcccgcctcc atggcctcgc ccgcgatgtc
     1261 ctcggcgcgc tccaggttgc ggatgaccag gtgccggtct tccttggcgc agggggcgag
     1321 ggtgaagccg cagttgccca tgaccacgct ggtgatgccg tgccagcagg acgaggtgcc
     1381 tagcgggtcc cagaagatct gggcgtccat gtgggtgtgc ccgtccacga accccggcgc
     1441 gacgaccctc ccctccgcgt cgatgacttc cttggcgctc tcgcggagcc gaccgatggc
     1501 gacgatcttg ccggccttca cgcccacgtc tccgcggtac cgcggcaggc cggatccgtc
     1561 caccacccat ccgttcttga tcagcaggtc ataggccatc gcgctggctc ctctccgccc
     1621 cgccccgggg gtccttctga gaaacggccc catggtaccc tacctgccgg tcccgccacc
     1681 agacgggagg agggcacatg gacctgggca ttttcgacca cctcgaccgg cgggacatcc
     1741 cggtggccga cttctacgag caccggctgc ggctgctcga gaagtacgac gcggcggggt
     1801 tctactcgta ccatctggcc gagcatcacg cgaccccgct cgggctggcg cccatgccgg
     1861 gcatcttcct ggccgcggcc acccagcgca cccgtcgcat ccgcctcggg ccctgcgtgt
     1921 actgtttgcc gctgtacaac ccgcttcgat tgatcgagga agtctgcatg ctcgatcatc
     1981 tctcgcgggg ccggttcgac ttcggggtgg gccgcggcat cgtcccgtac gagatggcct
     2041 tcttcgatct gcaccatctc gagaccgagg acatctaccg ggagaccctc gagatcgtcc
     2101 tgcaaggctt gacgagcgac gtgctcgatc atcgcgggtc gaagtacatc tacagaaagg
     2161 tgccgatgat cctgaagccg ctccagaagc cgcacccgcc gctctggtac gggctgggcc
     2221 acacggccgg cgccgagtgg gcggccgcca accgggtcaa cgtgatcacc aacctggggg
     2281 ccgagatgtc ggcgccgctc ttcgcccgct accgcgaggt gtggcagcgc aagcacggag
     2341 acgccgcgct accgcggctc ggcatgggcc gccacgtggt ggtggccccc accgaggacg
     2401 cggccatggc gctcggccgc gccagctacg cggtctggta cgccaacctc accaagctct
     2461 ggcgcgactt cggcgcgatc ccgttccgct tcgcgcccga cttcgacgag gcgcggcgcc
     2521 gcggggtagc catcgcgggc accccggccc aggtacgcgc ggagttcgag cggcaggtgg
     2581 cggcgagcgc ctgcacctac atgacctgcc ggctgatgtt cggcgagatg accgaggcgg
     2641 aggcggcggc caacgtcgac ctgttcgtcg ccgaggtcat gccgcacgtc accgcgctgg
     2701 ccgtgccggt gtgacgccat gaggctcaag gggcgcaccg cgttcgtcac cggggccagc
     2761 cgcaacatcg gccgcgccat cgcgctggcc ttcgcggccg agggcgcgga cctcgtgctc
     2821 aacacccgct ccaaccgcga cgagctggag gcggtggcgg aggagtgccg caagcgcggg
     2881 gtgcgcgccg cggccgtgct cggcgacatc ggcgatcccg acgcggtgga ggccatggtc
     2941 aggaacggtc tcgcggtgtt cggggccatc gacgtgctcg tgtgcaacgc ggccatccgg
     3001 ccccacaagt cggtcacgga gacctcgctg gaggactggc atcgcgtgtt cgcggtgaat
     3061 ctccactccg ccttctacct ggcgcgcgcg gtggtgcccg gcatgaagga gcgcgggcgg
     3121 gggagcatca tcgcgctcgg cgggcagtcg agcctcaccg ggcggccggg caccgccgcg
     3181 gtcaccgcgg ccaagaccgg gctgctgggc ctcgtgcggg cgctggccgc ggagctgggc
     3241 ccctcgggca tccgggtcaa catggtggtg ccgggcacca tcgacaccga gcgccgctac
     3301 gccgagtggt accctgagtt ccgtcaggcg ccgccgggcg gggcggagca attgaagcgc
     3361 atcccgctcg gccgcctggg ccgcccggag gagatcgcgg aggcgtgcct gttcctggcg
     3421 tccgacgcct ccgcgtacgt cacgggcgac acgatccggg tcatgggcgg cagttttatt
     3481 ggatagacga gaggagaaca acgtggccgc acaggcgatg gtgctcgagt tcggcatggg
     3541 cgtggacgtg cacggagacg aagggaccaa ggcggcgtgc cgtgcggtgt cggacgcgat
     3601 ccgacactcg agcctccccc tgttcaccga ggtgcgcgcc aagggcgggc gcatgctggt
     3661 ggacgtcacg gtgggtgtgc ccgatcccgg caaggtggac gtggagcgtg tgaagaagga
     3721 gctcccccac ggcgaggtga cggtgcggcc ggtgcagggc ggcctgcgcg tgccgaacgc
     3781 cgacaccatc atcgcgtgcg ccgcgatcac ggtgagcgtg gagtaccccg ggcgatgagc
     3841 gcccgggaac cgggcgcatc cccgttggag gtcgcgccat gagcggcatc gggctcaagc
     3901 ggctgcagca tctggtcctc tgggtgtccg acgtcgatcg cagcgtgcgc ttctactgcg
     3961 acgtgctggg cttcgaggtg aagacgcgat acccgaacgc cgccttcctg cgcatcgcgg
     4021 gcagcagcga ggatcaccac ctgggtctct tcgagcagcc cggtgtcacc gggcccgacg
     4081 agggcgcggt gcgcatgtac cactcggcgt gggaagtcgg cgagctcacc gacctggtac
     4141 gcgcccgtcg gcgcctgatc gaggcgcact cgctggtggg ctcctcggat cacggcgtga
     4201 gcctctcgct ctacgccaag gatcccgacg ggctcgagtt cgaggtgttc tggacggtgc
     4261 ccggcggccg tccggtgggc acgcgcgcgc tcgatctgga aggggagctg gcccgccgcg
     4321 gcatcccgat gccccaggag atcgacgtcg ccccgtcgac atgacgtgta gcggctgcgg
     4381 cttcgaagcg cccgccgact tcgcgttctg tccaaagtgc ggaggcaggc tggccgccac
     4441 tcccgcggcg ccaccgccgc cctcgccgcc cgccgcgccg gacagcgacc gccgcccggt
     4501 caccgtgctc ttcgcggacc tggccgggtt caccgcgctc agcgaggggc tcgatcccga
     4561 ggacgtgcgc gcgatccaga gcgatctttt ccgcgagatg tccgagtcga tcgagcgcta
     4621 cgaggggttc gtcgagaagt tcgtgggcga cgcggtcatg gcggtcttcg gcgccccgcg
     4681 cgcgcacgag gacgatcccg agcgcggcct gcgcgcggcc ctgctcatgc gcgaccggat
     4741 gggcacgctc aatcgccgct gggagcggcg ggtggggcgg ccgctcgcgc tgcacatcgg
     4801 gatcaacacc ggcccggtgg tggcgggccg gatcggcacg agcgccgacg ccgcctacgc
     4861 ggtgaccggt gacaccgtca acaccgcctc tcgcctgcag agcgcggcgc cgccgggcga
     4921 gatcctgatc agcgacgcga cctatcagct cacccatcac gcgttcgcgt tcgccccgcg
     4981 cgaggcgatc gcgctcaagg gcaagagcga gccggcgggg atccatcgcc tgctgggccc
     5041 gctcgcggcc ccgcgctcgg cccgcgggct cgagggcctc gggttggccg cgccgctcgt
     5101 gggccgcgag cgcgagctgc gccggatgga cgcggccttc gacgacatgc gggcggggcg
     5161 cgcccaggtg ctgagtctga tcggcgaacc cggggcgggg aaatcgcggc tgcagcggga
     5221 gttcttcgcg cgcctcgagg cggcgggacg cctcgagggc gtcacgatcc ggcgcgcgac
     5281 gtgctcggcg ctgggcgagc agacctacgg ggcggcggcc gcgctcctgc gcgacgccta
     5341 cggggtagag cacggcgatt ccgtcgaggt cgcgcggggt aagctcgcgg cgggcctgga
     5401 gacgctcggg gtcgacgcgg aggatcgggc ggcgatggta gccgcgctcg cccgggtgtt
     5461 cgggctcgag caggacgacg cgcgcatacg ccatctggag ccggaacagc tcaagcgcca
     5521 gatcttcatg gccacctacg ccctggtgga gcggcggctg gccgccggcc cgctggtgct
     5581 ggtcgtcgag gatctgcact gggccgacgc cgcctccatc gagctgctcg gtgtcgtcgc
     5641 ggaccgcctc gcggatcgcc cgctcctggt tctcctgatg tatcgaccga cgctcgagcc
     5701 ggatgcgctg ggcacgagcc ggaccccgca caccgcgatc gaggtcatgc ccctgtcgcg
     5761 gtccgatagc gaagacctgc tggccgcgtg gttcggggac tcgacgcatc tctttccgga
     5821 gcacctgcgg gtcctgatcc tggagcgagc gggcggcaac ccgctgtacc tcgaggaagt
     5881 ggtccgtgcc ctgctcgcgg ccggggtgct ggtccgcgac ggccaggtgt ggcgctgcac
     5941 cgcggaggcc gcgaccgcgc aggtgcccgc caccctgcac gggctgctcc tggcgcgcct
     6001 ggaccggctc gacgccgtgg agcggcgggt catccaggaa gcggcggtga tcggtcccca
     6061 cttcccggtg tcgctgctga aggcggcctc ggccgagccc gccgcggtgg acgcggcgct
     6121 cgacgcgctc gccggcgccg atctggtgac gccggggccg gatcaccagt tcctccacgg
     6181 ccttctccag gagatcgtct accagaacat cctcgtggcg cggcgcaccg agctgcacac
     6241 gcgcgtggcc accgccctcg aggcgcaggg agtgggcggc tccgagagtc tcgagcgact
     6301 ggtggccctc ggccaccact gggccctcgg ggccgacaag ggtcggggcg cgcggtacct
     6361 ggtgtcggcg ggtgactggg cgcgcggcat gtatgcgaac gcggacgcga tcctgcacta
     6421 tcagcgggcc ctcgacgcgc tcgcgcagtg cgacgcgccg gccgcggcca cgcagatcga
     6481 gaccgagcgg ctcgtggccc gggagcggat ggccgacctg ctcggcccga ccggacggcg
     6541 ggcggccgcg ctcggcgaat acgagatcgt cgaggcggga taccgcgcgg cgggggacgc
     6601 ggcggcccag gcgcggatcc ttcgcaagat ggggggcctc cactgggacg ccggcgcgcg
     6661 ggcccgggcg ctccagtgct tcgaggccgg gctcgccctg ctcggcgatc atcgagagca
     6721 catcgagcag gcgcacctct accaggagat gggccggctg gccttccgca gtggcgagag
     6781 ccagcgggcc gtcgagtggg cggagcaggc gcggctccac gccgagcggc tcgccgccga
     6841 ccccgcgctc
//
LOCUS       DASUFA010000242         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_503984, whole genome shotgun sequence.
ACCESSION   DASUFA010000242 DASUFA010000000
VERSION     DASUFA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503984"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VFX87_09070"
     CDS             complement(<1..1433)
                     /locus_tag="VFX87_09070"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX4995107.1"
                     /translation="MGQSLTRKLLDAHLVSGKPVAGEEIGLRVDQALLTDTNGTMAFL
                     QFEAMGFPRVRPSRVVAYIDHNVYQVDSRNSDDHRYLQTASSRYGAWFSKPGNGICHQ
                     VHFESFSVPGQFVLGTDSHTPLCGSTGMLAIGAGGLDVAVAMGGGPYHVVMPRVTSVW
                     LTGRLRPWVSAKDVIMELLRRYTVRGGSGRIFEYGGPGAATLSLPQRATICNMGAELT
                     LTTSVFPSDEATREYYRLLGREADWRPLAPDADAEYDDRIELDLSSLEPLVAMPGSPD
                     RVVPVAEVEGLPIEQVMVGSCTNSSWEDMQAVTRVLEGRKVAPSVSFVVFPGSHRILE
                     VMAREGLLATLLAAGATISEPTCGACAGIGHVPASGGRSLRAFNRNFPGRSGTKDDAV
                     YLCSPLTAAVSALAGRISDPRKAGTPPERHWPASLLASVAGLVPPLSASEAAGVEVVK
                     GPNIKPVPRGVPTEERLSAPVLIKLGD"
     gene            complement(1476..2606)
                     /locus_tag="VFX87_09075"
     CDS             complement(1476..2606)
                     /locus_tag="VFX87_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX4995108.1"
                     /translation="MTEGSQLIRGLEGVVAAETRLCDLDGRNGRLAYGGYDIDELARQ
                     ATFEEVAYLLWHDELPARALREKFQAELAAARPIPDALIRAFALMPRDTDPMRVLQAA
                     VAILGMHDPDATDNSRAANLRKAVRLTSQFTTAICAHHRVRSGLAPVPPSNELSHAAN
                     FLYMLTGEAPAAVTTRAVDASLTLYAEHELNASTFTTRVIAATLSDMHSAVAGGVGAL
                     KGTLHGGAGEAVMRTLLEVGTLDNVDRFVDRSMGEKRRLMGFGHRVYTAGDPRATILK
                     GLAEAACRQSGQALWYDLAVALHAKVQATKRLIPNVDFYSAPLFYSIGIPVDLFTPVI
                     AAARIAGWTANLLEQYDDNRLIRPRADYKGHPRRPFVPLEKR"
     gene            2760..>2979
                     /locus_tag="VFX87_09080"
     CDS             2760..>2979
                     /locus_tag="VFX87_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995109.1"
                     /translation="MVDDEPEVLELASEILKRVGYSVLEAADGAAALEVARLHEGEIH
                     VLVTDMVMPGMSGRDLAERLRALRTALPV"
BASE COUNT          419 a          998 c         1126 g          436 t
ORIGIN      
        1 ttgtcgccga gcttgatgag caccggcgcg ctcaggcgct cctcggtggg aactccgcgc
       61 ggcaccggct tgatgttcgg gcccttgaca acttcgaccc cggccgcctc ggacgccgac
      121 agcggcggga ccagtccggc caccgaggcc agcagggagg ccggccagtg ccgctcgggc
      181 ggcgtgccgg cttttcgagg gtcgcttatc cttcccgcca gcgcggagac ggcagcggtc
      241 agcggcgagc acagatagac cgcgtcgtcc ttcgtgccgc tccggcccgg gaagttgcgg
      301 ttgaaggcgc gcaggctccg cccgcccgag gcgggcacgt ggccgatgcc cgcgcaggcg
      361 ccgcaggtgg gctcggagat cgtggcgccc gccgcgagca gggtcgcgag caggccttcg
      421 cgcgccatca cctcgaggat gcgatggctg cccgggaaga ccacgaagga gaccgacggc
      481 gcgaccttgc ggccctcgag cacgcgggtc accgcctgca tgtcctccca cgaggagttg
      541 gtgcaggagc cgaccatcac ctgctcgatc gggagcccct cgacctcggc caccgggacc
      601 acgcgatccg gtgacccggg catggcgacc agcggctcga ggctcgagag atcgagctcg
      661 atccggtcgt cgtactccgc gtcggcatcc ggggccagcg gccgccagtc cgcctcgcgg
      721 ccgaggagcc ggtagtactc gcgcgtcgcc tcgtccgacg ggaacacgct ggtggtgagc
      781 gtcagctcgg cgcccatgtt gcagatggtc gcccgctggg gtagggaaag cgtcgccgcg
      841 ccggggccgc cgtattcgaa gatgcggccg ctgcccccgc gcaccgtgta gcggcgcagc
      901 agctccatga tcacgtcctt ggccgagacc caggggcgga gccgtccggt gagccagacc
      961 gaggtcacgc gcggcatgac gacgtgatac ggcccgccac ccatggccac cgccacgtcg
     1021 agaccacccg cgccgatggc cagcatgccg gtcgagccgc agagcggggt gtggctgtcg
     1081 gtgcccagca cgaactggcc ggggacgctg aaggactcga agtgcacctg gtggcagatg
     1141 ccgttgcccg gcttggagaa ccaggcgccg taccggctcg aggcggtctg gagatagcgg
     1201 tggtcgtccg agttgcgcga gtccacctgg tagacgttgt ggtcgatgta ggcgaccacg
     1261 cgggacgggc gcacccgcgg aaagcccatg gcctcgaact gcaggaacgc catggtcccg
     1321 ttggtgtcgg tgagcagggc ctgatccacg cggagcccga tctcttcgcc ggccaccggc
     1381 ttgccggaga ccaggtgcgc gtcgaggagc ttgcgcgtca ggctctggcc catgggggtt
     1441 attgcggact accgtttcga tctgcgctca tcttcttacc gcttctccag ggggacgaag
     1501 gggcggcggg ggtggccctt gtagtcggcg cgggggcgga tcaggcggtt gtcgtcgtac
     1561 tgctcgagca ggttggcggt ccaccccgcg atccgggccg ccgcgatcac cggggtgaag
     1621 agatccaccg ggatgccgat cgagtagaag agcggcgccg agtagaagtc cacgttgggg
     1681 atgaggcgct tggtcgcctg caccttggcg tggagcgcga ccgccaggtc gtaccagagg
     1741 gcctggccgg actggcggca ggccgcctcg gcgaggccct tcaggatggt cgcgcgcggg
     1801 tcgcccgcgg tgtagacacg gtggccgaag cccatgaggc gccgcttctc gcccatcgag
     1861 cggtccacga agcggtccac gttgtcgagc gtgccgactt ccagcagcgt gcgcatcacc
     1921 gcttcgccgg cgccgccgtg gagcgtgccc ttcagcgcgc ccaccccgcc cgccaccgcc
     1981 gagtgcatgt ccgagagcgt ggccgcgatg acgcgggtcg tgaaggtgga cgcgttcagc
     2041 tcgtgctcgg cgtagagcgt caggctcgcg tccaccgcgc gggtggtgac cgcggccggc
     2101 gcctccccgg tgagcatgta caggaagttg gccgcgtggg agagctcgtt cgagggcggc
     2161 acgggggcga gcccgcttcg cacccggtgg tgcgcgcaga tggccgtggt gaactggctg
     2221 gtcaggcgca ctgccttgcg caggttggcc gcgcgcgagt tgtcggtggc gtccggatcg
     2281 tgcatgccga ggatcgccac ggcggcctgg agtacccgca tgggatcggt gtcgcgcggc
     2341 atgagggcga aggcgcggat cagcgcgtcg gggatgggcc gcgccgccgc cagctcggcc
     2401 tggaacttct cgcgcagggc ccgcgcgggc agctcgtcgt gccagagcag gtaggccact
     2461 tcctcgaagg tggcctgtcg cgcgagctcg tcgatatcgt agccgccgta ggccaggcgg
     2521 ccgttccgcc cgtccaggtc gcagagacgc gtctcggcgg ccaccacgcc ctccaggccc
     2581 cggatgagct ggctgccctc ggtcatcgtt gactcctctc gcagcggcga gcattccgcg
     2641 gacggcgtac gcgggcccgc gtgcgcagtt gacggtatac ccgcggcccg gcacagcgca
     2701 agagcgttgg tggcgcgcgc gccgctgagt aagatcgaac cacgtcacga acagtcctgg
     2761 tggtggacga cgagccggag gtgctggagc tggccagcga gatcctgaag cgcgtcggct
     2821 actcggtgct cgaggccgcg gacggcgcgg ccgcgctcga ggtggcgcgg ctccacgagg
     2881 gggagatcca cgtgctcgtg accgacatgg tgatgccggg catgtcgggc cgcgacctgg
     2941 ccgagcggct gcgggcgctg cggacggcgt tgccggtgc
//
LOCUS       DASUFA010000243         4703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1300964, whole genome shotgun sequence.
ACCESSION   DASUFA010000243 DASUFA010000000
VERSION     DASUFA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300964"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFX87_09085"
     CDS             complement(<1..951)
                     /locus_tag="VFX87_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995110.1"
                     /translation="MSTTLAVASGAHLVGSVPLASAEAVFRAVADEIGDRLRRIPDGE
                     TGPRSDWIVWQLPVFTTQRQLEVVPPGPDSWRPLPRVRLAEGARAESVAFGALGYADA
                     ALSSYRVFARLKRDGVVPVACRFQVCLPTPLAPISAFVVPEHQELLEPVYEAQVLGEL
                     RIILQEVPHDQLAIQWDTNFEFGMLEGVFPVWFGDVKGGILERLLRISRHVPPDVPIG
                     YHLCYGDVQHRHFKEPADAGKLVEVANALAASLGRPLNWIHLPVPRERLDEAYYAPLR
                     DLRLRAESELYLGLLHHTDGVEGTRRRIEVAHRFVPDFGVA"
     gene            complement(961..2313)
                     /locus_tag="VFX87_09090"
     CDS             complement(961..2313)
                     /locus_tag="VFX87_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4995111.1"
                     /translation="MPTTEATIEAESTELLRTLIRNACVNTGEESSGGEARSCDALEA
                     FFAGSGLACERYTSSPGRMSLITRIQGSDPKAPALLLMGHTDVVPVSPSGWKRDPFAA
                     DLVDGVVWGRGAIDMLNLTATMAVATRRLRTTDWKPRGTLIYLAVADEEAGGVYGAEH
                     LVEHEFDAVKCDYVVTESGGVPIPTPSGPKLRVSVGEKGGNWRRLVVHGTPGHGSRPF
                     RTDNALVTAAVVIQRIADYRPTARILDAWKNYITALELDPDLTAALVDPDRVWKTARD
                     MDNATLAREAHACTHTTFSPNVVRGGSKVNVIPDRVEIQVDIRALPGITSEEVEAMLK
                     EAMGDLADRVTIDTPPNRRRGGSLSTIDTPLMRALSRASGKLMPGSTVVPAMTTGGTD
                     ARFFRWKGIPAYGFGLHSTRIPYTEYPVMFHGHNERVDTESLRLSAMMWEELCRDFLG
                     "
     gene            complement(2316..3269)
                     /locus_tag="VFX87_09095"
     CDS             complement(2316..3269)
                     /locus_tag="VFX87_09095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4995112.1"
                     /translation="MPPAPLTFGCHLPVFGPIATRGNVIAFARKMEALGYESLWASDH
                     VVLPYTITSKYPYNPSGQFPLPPTAAFLEPLTTLALVAGVTERAQLGTTILVLPHRHP
                     VLAAKMLATLDHLAEGRVILGVGVGWMREEIEILGGDYDRRGAWSDEAIRIMRACWSE
                     EKPHYKGEFFSFDEIGVVPQPPRKAIPIWVGGHTPRALRRVAELGDGWHAAFTPPDAF
                     ERDIARLRAECAKVNRRLDDLAITLRVGLSFRDTPGGADRKPLQGTPDQIVADIRRYR
                     DLGVRSFLLEARYRDLDDMVAIFERFAGEIRPAVDAGPRRT"
     gene            complement(3273..>4703)
                     /locus_tag="VFX87_09100"
     CDS             complement(3273..>4703)
                     /locus_tag="VFX87_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4995113.1"
                     /translation="GDKVCLIYPTCAEFFYTFFGALRLGAVPVPLYPTLGVEATANVF
                     RDSEAKAVATIGWFRPGVDESAAGAANIRHILEPSDLEADAPVPPFPAIREDDVAFIQ
                     YTSGSTGSPRGVVLSHANVCRTVEFMGEAAQLGREDTVVSWLPLYHDMGLIGCAFTPP
                     TTGAPLYLLPPDLKNPRVWLELVTRVRATFTVSPDFGYRNCVRNIGDTSGIELSSLKQ
                     ALSGAEPVRLSTIEAFERKFDIDNVITPCYGLAEATLAVAIWPRFTPLRLDTSGKFLS
                     VGHACRGVSVRIMQEDRVVGPGVEGEIVVRSPGVMQGYHNNPEATRRVLSADGWLRTG
                     DLGFLDAEEYLYVTGRVKDLIILGGENVIPADVEEVVDRVGGVRYSAAVGIDSERTGS
                     QRLHVVAEVRGDGGTRDEQSAIVREIVQRVHHASGHRPAKVLLVRPSTIPKTSSGKIQ
                     RARLGQMIAGGEITEQIVYASGGHAR"
BASE COUNT          693 a         1587 c         1750 g          673 t
ORIGIN      
        1 ggcgaccccg aagtccggca cgaagcggtg ggccacctcg atgcggcggc gggtgccctc
       61 gaccccgtcc gtgtggtgca gcaggcccag gtagagctcg gactccgcgc gcaggcggag
      121 atcccgcagg ggcgcatagt acgcctcgtc gaggcgctca cgcgggacgg gcaggtggat
      181 ccagttcagc gggcgcccca ggctcgccgc cagcgcattg gcgacctcca ccagcttgcc
      241 ggcgtcggcc ggctccttga agtggcggtg ctgaacgtcg ccgtagcaga ggtgatagcc
      301 gatcgggacg tccgggggca cgtggcggga gatccggagc agccgctcca ggatcccgcc
      361 cttgacgtcg ccgaaccaga ccgggaatac gccctcgagc atcccgaact cgaaattggt
      421 gtcccactgg atggcgagct gatcgtgggg cacttcctgg aggatgatcc gcagctcgcc
      481 gagcacctgc gcctcgtaga cgggctcgag cagctcctgg tgctcgggca ccacgaaggc
      541 gctgatgggg gccagcgggg tggggaggca gacctggaag cgacaggcca ccggcaccac
      601 gccgtcgcgc ttgaggcgcg cgaagacccg gtaggaggac agcgccgcgt ccgcatagcc
      661 gagcgccccg aaggctacgc tctccgcgcg tgccccctcg gccaggcgga cgcggggcag
      721 cggccgccag ctgtccgggc cgggcggcac cacctcgagc tggcgctggg tggtgaagac
      781 ggggagctgc cacacgatcc agtcggaccg cgggccggtc tcgccgtcgg ggatgcgccg
      841 aaggcgatcc ccgatctcat cggcgacggc gcggaacacg gcctccgcgc tggccagcgg
      901 cacgctgccg accagatggg ctccagatgc cacggcaaga gtggtgctca tggcggcggc
      961 ctaccccagg aagtcgcggc agagctcttc ccacatcatg gccgagaggc gcaggctctc
     1021 ggtatccacg cgctcgttgt ggccgtggaa catgaccggg tactcggtgt aggggatgcg
     1081 ggtggagtgc aggccgaagc cgtaggcggg gatgcccttc caccggaaga agcgcgcgtc
     1141 ggtgccgccg gtggtcatgg ccggcaccac ggtgctgccg ggcatgagct tgccggacgc
     1201 gcgcgacagc gcgcgcatga gcggcgtgtc gatggtggac agcgagccgc cgcgccgccg
     1261 gttgggcggc gtgtcgatgg tcacgcggtc ggccaggtcg cccatggcct ccttcagcat
     1321 ggcctccact tcctccgagg tgatgccggg cagcgcgcgg atgtccacct ggatctcgac
     1381 gcggtcgggg atcacgttca ccttgctacc cccgcggacc acgttgggcg agaacgtggt
     1441 gtgggtgcag gcatgggcct cgcgggccag cgtggcattg tccatgtcgc gcgcggtctt
     1501 ccagacccga tcggggtcca cgagggcggc ggtgaggtcg gggtccagct ccagcgccgt
     1561 gatgtagttc ttccacgcgt ccaggatgcg cgcggtgggc cggtagtcgg cgatgcgctg
     1621 gatgacgacg gccgcggtga ccagggcatt gtcggtccgg aacgggcgcg agccatggcc
     1681 cggcgtgccg tggacgacca ggcgccgcca gttgccgccc ttctcgccga cggatacgcg
     1741 gagcttgggg ccggagggcg tcgggatggg caccccgccg ctctcggtca ccacgtagtc
     1801 gcacttgacc gcgtcgaact cgtgctccac gagatgctcg gccccgtaga cgccgcccgc
     1861 ctcctcgtcc gccaccgcga ggtagatgag ggtcccgcgg ggcttccagt cggtggtgcg
     1921 gagccggcgc gtggccaccg ccatggtcgc ggtgaggttg agcatgtcga tggccccgcg
     1981 gccccagacc acgccgtcca ccaggtcggc ggcgaaggga tcgcgcttcc agcccgacgg
     2041 gctcaccggg accacgtcgg tatggcccat gaggaggagg gccggcgcct tgggatcgct
     2101 gccctggatg cgggtgatga ggctcatgcg ccccggggac gacgtatagc gctcgcaggc
     2161 gagccccgac cccgcgaaga aggcctccag cgcgtcgcag ctccgcgcct cgccccccga
     2221 ggactcctcg cccgtattca cgcaggcatt gcggatgagg gtgcggagca gctcggtgga
     2281 ctcggcctcg atggtggctt cggtggtcgg catggtcagg tcctccgagg gccggcatcg
     2341 acggcggggc ggatctcgcc ggcgaagcgc tcgaatatcg ccaccatgtc gtcgaggtcg
     2401 cgatagcggg cttcgagcag gaaggagcgg acgccgaggt ctcggtagcg ccggatgtcg
     2461 gcgacgatct ggtcgggcgt gccctgcagc ggcttgcggt ccgcgccgcc gggcgtgtcg
     2521 cggaaggaca gccccacgcg gagcgtgatg gccaggtcgt ctaaccgacg gttgaccttc
     2581 gcgcattcgg cgcggagccg cgcgatgtcg cgctcgaagg cgtccggggg cgtgaaggcg
     2641 gcatgccagc cgtcgcccag ctcggccacg cggcgcaggg cccgcggggt atggccgccc
     2701 acccagatgg ggatggcctt gcgcgggggc tggggcacca cgccgatctc gtcgaaggag
     2761 aagaactcgc ccttgtaatg cggcttctcc tccgaccagc aggcgcgcat gatgcggatg
     2821 gcctcgtcgg accaggcgcc ccgccggtcg tagtcgccgc ccaggatctc gatctcctcg
     2881 cgcatccacc ccacgcccac gccgaggatg acgcggccct cggccaggtg gtcgagcgtg
     2941 gccagcatct tcgccgcgag caccgggtgg cggtgcggca ggaccaggat ggtggtgccg
     3001 agctgcgcgc gctcggtcac gccggccacc agggccaggg tggtcagcgg ctcgaggaag
     3061 gcggcggtcg gcggcagggg gaactggccg ctgggattgt agggatactt cgacgtgatc
     3121 gtgtagggga gcaccacgtg gtcgctggcc cacagcgact cgtagcccag cgcttccatc
     3181 ttgcgcgcga aggcgatcac gttcccgcgc gtggcgatgg gaccgaatac cggcaggtga
     3241 cagccgaagg tcagcggagc gggcggcatc ggctagcggg cgtgcccgcc cgacgcgtag
     3301 acgatctgct cggtgatctc gccgccggca atcatctgac ccagccgggc gcgctggatc
     3361 ttgccgctgg acgtcttcgg gatggtgctg ggccgcacca gcagcacctt ggcgggacgg
     3421 tgcccactgg cgtggtgcac gcgctggacg atctcgcgca cgatcgccga ctgctcgtcg
     3481 cgggtgccgc cgtcgccgcg cacctccgcc acgacgtgca ggcgctggct gccggtgcgc
     3541 tcgctgtcga tgcccaccgc ggccgagtag cggacgcccc ccacgcgatc caccacctct
     3601 tccacgtcgg cggggatcac gttctcgccg cccaggatga tgaggtcctt gacccggccc
     3661 gtgacgtaga gatactcctc ggcatcgaga aagcccaggt cgcccgtccg gagccagccg
     3721 tcggccgaga gcacgcggcg ggtggcctcg gggttgttgt ggtagccctg catgacgccc
     3781 gggctccgga ccacgatctc gccttccacg cccggcccca ccacccggtc ctcctgcatg
     3841 atgcgcaccg acacgccgcg gcaggcatgc cccaccgaca ggaacttgcc ggaggtgtcc
     3901 agacgcaggg gcgtgaagcg gggccagatc gccaccgcca gggtggcctc ggccaggccg
     3961 tagcagggcg tgatgacatt gtcgatgtcg aatttccgct cgaaggcctc gatggtgctg
     4021 agccgcaccg gctcggcgcc ggacagcgcc tgcttgagcg aggagagctc gatgccgctg
     4081 gtatcgccga tgttccgcac gcagttccgg tagccgaagt ccggcgatac cgtgaaggtg
     4141 gcccgcacgc gggtgacgag ctccagccag acccgcgggt tcttgagatc cggcggcagg
     4201 aggtagagcg gcgcgcccgt ggtgggaggg gtgaaggcgc agccgatcag ccccatgtcg
     4261 tggtagagcg gcagccacga caccacggtg tcctcgcggc cgagctgggc cgcctccccc
     4321 atgaactcca ccgtgcggca tacgttggcg tggctcagca ccacgccgcg cggggacccc
     4381 gtgctgccgg acgtgtactg gatgaaggcg acgtcgtcct ctctgatggc ggggaatggg
     4441 ggaacggggg cgtcggcctc gaggtccgac ggctccagga tgtgccggat attcgccgcc
     4501 cccgccgcgg actcgtccac gcccggccgg aaccagccga tggtggccac ggccttggcc
     4561 tcggagtcgc ggaagacatt ggcggtggcc tccacgccga gcgtgggata gagcggcacc
     4621 ggcacggcgc cgagccggag cgcgccgaag aacgtgtaga agaactccgc gcaggtgggg
     4681 tagatcaggc agaccttgtc ccc
//
LOCUS       DASUFA010000244         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1301282, whole genome shotgun sequence.
ACCESSION   DASUFA010000244 DASUFA010000000
VERSION     DASUFA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301282"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFX87_09105"
     CDS             complement(<1..300)
                     /locus_tag="VFX87_09105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4995114.1"
                     /translation="MKPLPHRYQVRLDGGTTGYAALSAEGLPNLASAPPIEFGGPGDA
                     WSPEHLLLAAVTTCFMFTLRAVAQASRVDFISLDLAVEGVVERRESQLRFTEIVLR"
     gene            complement(514..1611)
                     /locus_tag="VFX87_09110"
     CDS             complement(514..1611)
                     /locus_tag="VFX87_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX4995115.1"
                     /translation="MRITAAVLHDVNKPYSIETVELDAPRRGEVLVKVAAAGVCRSDL
                     HFQKGEATIALPAVLGHEGSGTVEAVGEGVTLVKSGDRVILSFVPNCGHCPSCETGRP
                     HLCDEHARTTGKLFDNTSRLHTLAGQDLAHMGKVACFADHAVVPEAGCIPAPAGLDFP
                     QMALIGCSVTTGVGAALFNAGVQPGDSVAVIGCGGVGLNVLQGARLAGATTIVAVDVK
                     AAALDFARKFGATHTVNARTEDAVERVKAITGGGAHWAFEAYGGGATTRMAVDMVRKR
                     GTAVIVGIAPIGDHAAIEPVVVTRMEKTIRGCYYGTARPRQDMPRIADWYGRGLLDID
                     GLVTRRYPLADINRAYEDLDTDAVGRGVIVF"
     gene            complement(1621..2964)
                     /locus_tag="VFX87_09115"
     CDS             complement(1621..2964)
                     /locus_tag="VFX87_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995116.1"
                     /translation="MRAHFLTRRLFDGTALHTGRALRLTVDGDRIAYVGDHASGPAVA
                     TGEEVVDLTGCSLLPGLIDIHVHLSYGNAEANEDIDIYAPFEYRALRAMVYAQQVLMA
                     GYTSIADPASTGRVSVAVRDAINAGWYVGPRITAAGRQLTSRQGLGDWYPPWIGVPES
                     SIGALVRSPEEAKEEIRQQVKDGVDLIKIDFDGTQTNPRTGHLVACFDQAEASAMVAE
                     AQRLGRRVVVHAVDREAVLYAARAGADVIFHAFDMDDECLEAIVANGCMLSPALTFLY
                     NAIEFTQPSDPSFAWRPARSRKVVERAVEVLGAARKAGVPFLVGTDSGFAVTPYGEWH
                     ARELQIMVDDLGFTPEEALTATTVNNARILREHGEVGGLAPGKLADFVVVKGDPVADV
                     GVLMKPGAIEAVYLGGTRTALAPPAAARRYSWESSHRQWGEVYNRVRVAQLRAAR"
     gene            complement(3001..4404)
                     /locus_tag="VFX87_09120"
     CDS             complement(3001..4404)
                     /locus_tag="VFX87_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995117.1"
                     /translation="MTHTDIKGLAVSTSSSEALAAYERGLDLFLRWRGGPMEALGAAV
                     KADPGFVLAHCARALIAWRMGQVAAAAAAHQEVMALADRVHDERERAHVAVVDALQLG
                     ERAKAQDLLERTCERYPLDRLIVRIVGLNCIAQGDYEGGMTIARRSLDVDPGEPQFMT
                     MLGFFLEQSGYNEEGLEMSSRSLALDPTNLYTYHAVGHAYQARGDYPRALETFECAAS
                     LERHTHVLWHLADARAILGHERFTRDYWSSTAPVLPLYERVELLWRLEMLHHVATPDA
                     VWKDLAAEGERLLEHADALTLWMHHWIGLAFARAGETRKAERQVTYLRSLPEGKPSGY
                     WSTVGADILEGELAILRGDEASAVGLMATAIGRIHAMGGGSREQKDIFRDVFLELHRR
                     LGRTGDVTALAQERLLQNPNHLQSLAALAWAYGRTGHVAEQRQAWSQFVRKAEAVGLA
                     PDAPALAEARRVLHAVA"
     gene            4564..4980
                     /locus_tag="VFX87_09125"
     CDS             4564..4980
                     /locus_tag="VFX87_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4995118.1"
                     /translation="MSASGRAPGSLVKALAALVLLAVGAGGCASASTPDRVTPEALLA
                     SIGAGTAPVIVDVRTRREYEAGHVPGALHVPFYTLLVRQDDIPGPRERVVVVYCEHGP
                     RAGVARFALRLSGFTDVRYLDGHMSGWKERGLLLSR"
     gene            complement(4970..>5212)
                     /locus_tag="VFX87_09130"
     CDS             complement(4970..>5212)
                     /locus_tag="VFX87_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995119.1"
                     /translation="RPRPPGSQELADATRPYYELTIEKFGVDRCMFESNFPVDAVSCS
                     YAVLWNSFKRLASKYSAVEKAKLFHDTAARVYRLTG"
BASE COUNT          703 a         1833 c         1917 g          759 t
ORIGIN      
        1 ccgcagcacg atctcggtga agcgaagctg gctctcgcgc cgctcgacca ccccttccac
       61 cgcgaggtcc agagaaatga agtcgacccg ggaggcctgc gccaccgcgc gcagcgtgaa
      121 catgaagcag gtcgtcaccg cggcaagcag gagatgctcg gggctccagg cgtcgcccgg
      181 gccgccgaac tcgatcggcg gcgccgacgc caggttgggc aggccctccg cggacagcgc
      241 cgcgtagccg gtggtgccgc cgtcgaggcg aacctggtag cgatggggca gtggcttcat
      301 gggatgcgat ctctccccac ggcaggagaa cagcaaagtt gctgccagcc gtgagccgcc
      361 gcccttcgcg gccgggcgcc ttgccgcccc ggtacttccg gcgcaggtgg tcgctcggcc
      421 gcgggcggcc gccgcgctgg ctgtggaggc tctgccccgg gtggggcacg ggcgggacaa
      481 gaccggggtg cccgtgggcc gccccgcacc ctctcagaag acgatgacgc cgcggccgac
      541 cgcgtcggtg tcgaggtcct cgtaggcccg gttgatgtcg gcgagcgggt agcgccgggt
      601 gaccaggccg tcgatgtcga gcaggccacg gccgtaccag tcggcgatcc tcggcatgtc
      661 ctggcggggc cgggcggtgc cgtagtagca gccgcggatg gtcttctcca tccgagtgac
      721 gacgaccggc tcgatcgcgg cgtggtcgcc gatcggggcg atgcccacga tgaccgcggt
      781 gccccgcttt cgcaccatgt cgacggccat gcgggtggtg gcgccgccgc cataggcctc
      841 gaaggcccag tgcgcgcccc cgccggtgat ggccttcacg cgctcgaccg cgtcctcggt
      901 gcgcgcgttc acggtgtggg tggcgccgaa cttccgcgcg aagtcgagcg ccgcggcctt
      961 gacgtcgacg gccacgatcg tcgtggcccc ggcgagccgc gcgccctgca ggacattgag
     1021 gcccacgcca ccgcacccga tgaccgcgac gctgtcgccc ggctgcacgc cggcgttgaa
     1081 cagcgcggcg ccgacgccgg tggtgacgct gcagccgatg agcgccatct gggggaagtc
     1141 gaggccggcg ggcgccggga tgcagccggc ctcgggcacc accgcgtggt cggcgaagca
     1201 cgccaccttg cccatgtggg ccaggtcctg cccggcgagg gtgtgcaggc gcgaggtgtt
     1261 gtcgaagagc ttgccggtgg tgcgggcatg ttcgtcgcaa aggtgcggcc gcccggtctc
     1321 gcacgagggg cagtggccgc agttgggcac gaagctcagg atgacgcggt cgccggactt
     1381 gaccagcgtg acgccctcgc ccaccgcctc gaccgtgccg gagccctcgt ggcccagcac
     1441 ggcgggcagg gcgatggtcg cctcgccctt ctggaagtgc aggtcactgc ggcagacgcc
     1501 ggcagcggcc accttgacga ggacctcgcc gcgccggggc gcgtcgagct ccacggtctc
     1561 gatcgagtag ggcttgttga cgtcgtggag cacggcggcc gtgatgcgca tgatgtcctc
     1621 ctagcgcgcc gcccgcagct gggcgacgcg gacgcggttg tagacctcgc cccactgccg
     1681 gtggctgctc tcccacgaat agcgacgtgc cgcggcgggc ggggccagcg cggtccgcgt
     1741 gccgccgaga tacactgcct cgatggcgcc cggcttcatg agcacgccca cgtcggccac
     1801 cgggtcgccc ttgaccacga cgaagtcggc gagcttgccg ggggccagcc cgccgacctc
     1861 gccgtgctca cgcaggatcc gggcattgtt cacggtggtg gcggtgagcg cctcttccgg
     1921 cgtgaagccc aggtcgtcga ccatgatctg cagctcgcgc gcgtgccact cgccgtaggg
     1981 cgtgaccgcg aagccgctgt cggtgccgac caggaagggg acgcccgcct tgcgcgcggc
     2041 gcccaggacc tcgacggcgc gctccaccac cttccggctg cgcgcgggcc gccaggcgaa
     2101 ggagggatcc gagggctggg tgaactcgat ggcgttgtag aggaaggtga gggccggcga
     2161 cagcatgcag ccgttggcca cgatggcctc gaggcactcg tcgtccatgt cgaaggcgtg
     2221 gaagatcacg tcggcgcccg cccgggccgc gtagaggacc gcctcgcggt cgaccgcgtg
     2281 cacgaccacg cggcggccga gccgctgagc ctcggcgacc atcgcgctgg cctcggcctg
     2341 gtcgaagcag gccaccaggt ggccggtgcg cggattggtc tgggtgccgt cgaagtcgat
     2401 cttgatcagg tcgacgccgt ccttcacctg ctggcggatc tcctccttcg cctcctccgg
     2461 gctgcgcacc agcgcgccga tcgacgactc gggcacgccg atccacgggg gataccagtc
     2521 gccgaggccc tggcgcgagg tgagctggcg gccggccgcg gtgatgcgcg ggcccacgta
     2581 ccagcccgcg ttgatggcgt cgcgcaccgc cacgctgacg cgccccgtgc tcgccgggtc
     2641 ggcgatcgag gtgtagccgg ccatgagcac ctgctgggca tagaccatgg cgcgcagcgc
     2701 ccggtactcg aacggcgcgt agatgtcgat gtcctcgttg gcctcggcgt tgccgtagct
     2761 caggtggacg tggatgtcga tgaggccggg caggagggaa cagcccgtga gatccacgac
     2821 ctcctctccg gtcgcgacgg cgggcccgga ggcgtggtcg ccgacgtagg caatccggtc
     2881 gccgtcgacg gtgagccgaa gggcccggcc ggtgtgcaag gcggtcccgt cgaagaggcg
     2941 acgggtcagg aagtgggcgc gcatggccgg gtcctctcgt gtcgtgccgg agccggtcgc
     3001 ctaggcgacg gcgtgaagga cgcgccgggc ctcggcgagc gccggcgcgt cgggggcgag
     3061 accgacggcc tcggccttgc ggacgaactg gctccaggcc tgccgctgct cggcgacgtg
     3121 gccggtgcgc ccgtaggccc acgccagcgc ggccagcgac tgcaggtgat tgggattctg
     3181 cagcaggcgc tcctgggcca gcgccgtcac gtctcccgtg cggccgagac gccggtgcag
     3241 ctcgaggaac acgtccctga agatatcctt ctgctcccgg ctgccgccgc ccatggcgtg
     3301 gatgcggccg atcgccgtcg ccatcagccc taccgcggag gcctcgtcgc cgcgcaggat
     3361 ggcgagctcg ccttcgagga tgtcggcgcc gaccgtggac cagtacccgc tcggctttcc
     3421 ctcgggcagg ctccgcagat acgtgacctg ccgctcggcc ttccgcgtct ccccggcgcg
     3481 ggcgaaggcg agtccgatcc agtgatgcat ccagagggtc agggcgtccg cgtgctcgag
     3541 cagccgctcc ccctcggcgg ccaggtcctt ccagaccgcg tccggcgtcg ccacgtggtg
     3601 cagcatctcg agccgccaga ggagctccac gcgctcgtag agcggcagga cgggggcggt
     3661 ggagctccag tagtcgcgcg tgaaccgctc gtgccccagg atggcccggg catcggcgag
     3721 atgccacagg acgtgggtgt ggcgctcgag cgaggcggcg cactcgaacg tctcgagcgc
     3781 cctcggatag tccccccggg cctgataggc gtgccccacc gcgtggtacg tgtagagatt
     3841 ggtggggtcg agggcgagcg agcgggagct catctcgaga ccttcctcgt tgtagcccga
     3901 ctgctcgagg aagaagccca gcatggtcat gaactggggc tcacccgggt ccacgtcgag
     3961 gctccggcgg gcgatggtca tgcccccctc gtagtcgccc tgggcgatgc agttgagacc
     4021 gacgatacgc acgatcagcc ggtcgagcgg gtaccgctcg cacgtgcgct ccagcaggtc
     4081 ctgggccttc gcgcgctccc ccagctggag cgcgtcgacc accgccacgt gggcccgctc
     4141 gcgctcgtcg tggacccggt cggccagggc catgacttcc tggtgggccg ccgccgccgc
     4201 cgcaacctgg cccatgcgcc aggcgatgag tgcccgcgcg cagtgggcga ggacgaaccc
     4261 gggatccgcc ttcaccgccg cgcccagtgc ttccatcggg ccgccgcgcc agcgcaggaa
     4321 gagatccagc ccgcgctcgt atgccgcgag cgcttccgag gacgacgtgg agaccgccag
     4381 ccccttgata tcggtgtgag tcatgagccg tcccgtcgtg gacgtcgtgg cgatcgagca
     4441 gcgctcgttg ccgtacgcgg ggatcctaca ccacggcggc cggcccctca actccacggt
     4501 ctgccctgcc ggtcgcgggc aaatgtcgtg gcgacggggg ccggtggctt accgtatgga
     4561 gcgatgagtg cttccgggcg agcgccgggt tcgctggtca aggctctcgc ggcgctcgtg
     4621 ctcctggcgg tcggggcggg cggctgtgcg agcgcctcca cccccgaccg ggtgacgccg
     4681 gaggcgttgc tcgcctcgat cggcgccggc accgccccgg tcatcgtgga cgtccggacc
     4741 cgccgcgagt acgaggccgg tcacgtcccg ggcgcgctcc acgtgccctt ctacacgctg
     4801 ctcgtccgcc aggacgacat cccgggcccg cgggagcgcg tggtggtcgt gtactgcgag
     4861 cacggcccgc gcgccggtgt cgcccggttc gccctgcgcc tgtccggctt cacggacgtg
     4921 cgctacctcg acgggcacat gtcgggctgg aaggagcggg gcctgctcct cagccggtga
     4981 ggcgatagac ccgggcggcg gtgtcgtgga agagcttggc cttctccacc gccgagtact
     5041 tcgacgccag acgcttgaac gagttccaca gcaccgcgta gctgcagctc accgcgtcca
     5101 cggggaagtt cgactcgaac atgcagcgat cgacgccgaa cttctcgatc gtgagctcgt
     5161 agtagggtcg ggtcgcatcc gccagctcct ggctcccggg cggccggggc cg
//
LOCUS       DASUFA010000245         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_631360, whole genome shotgun sequence.
ACCESSION   DASUFA010000245 DASUFA010000000
VERSION     DASUFA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631360"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFX87_09135"
     CDS             complement(<1..938)
                     /locus_tag="VFX87_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4995120.1"
                     /translation="MATITGSEVLAQALRSQGVDTMFYLMGGPMLETEAACIKLGIRA
                     IDTRHEQAAALAAHAWTRVTRRPGVAMGCSGPGAINLATGVANAFTDCAPLIAIGGSS
                     PRVYLGMEAFQEIDQVALMKPITKWTERIYDARRIPDVVDTAFRQATSGRPGPVYIDM
                     PGDILGEKIEEEQLHYPGPWRPAPRSLGDPSAVREAINVLSRAERPVIIAGSGVWWSD
                     GAAALQAFVEATGIPFYTTPISRGLIAEDHELAFLNARSKAFTEADVVLAVGTRFNWV
                     IQFGRPPRFAADLKVIHVDINPTQLGHNRSVDVPIAG"
     gene            1019..2215
                     /locus_tag="VFX87_09140"
     CDS             1019..2215
                     /locus_tag="VFX87_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4995121.1"
                     /translation="MVHVQFNPMDPAFVADPYPTYHRLRAEDPVHHSPLGFWVLTRYP
                     DVMAMLRDPRLIKEPIAAFVAARFGMAAPPPGLGLSMLDRDPPDHTRLRGLVSKAFTP
                     KALESLRPHIQQIVDDLLADAAGKGQMDLIEEFAYPLPVRVICEMLGVPVKDHERFKA
                     WGLDIARGLDAIMLPPDSEVGQRSVSGRRALAEYFRALIAERRAAPRDDMLSALIAAE
                     EAGDKLNEEELLATCILLLVAGHETTVNLIGNGTLALLRHPDQLQKLRASPGLIATAV
                     EELLRFDGPVQRTARIPSEDITIGGRTIPKGDMVMPFLGAADRDPAQFPDPDRLDITR
                     ADNRHIAFGMGIHFCLGAPLARMEGQIAINALLARLPKLALATDRPQFRQSLTLRGLQ
                     ALPVSF"
     gene            2212..3354
                     /locus_tag="VFX87_09145"
     CDS             2212..3354
                     /locus_tag="VFX87_09145"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX4995122.1"
                     /translation="MRTARVAAVLPALVLLVLPALAQEAPRSPTPAAVKLAATVTDVA
                     RGLEHPWGVELLPDGRFLVTERPGRLRIVDRDGRLSVPLIGVPEVYARGQGGLLDVAL
                     GPGFAQDRLVYFSFSEPGPGGAGTAVARGRLSERGLEGTQVIWRQQPKVEGANHWGSR
                     LVFRADGTLFVTVGDRYAYRDRAQDLSTTLGKVVRINPDGSVPRDNPFVGRAGALPEI
                     WSLGHRNVQAAVLDARGELWTVEHGARGGDELNNPQPGKNYGWPVITYGVDYSGARIG
                     IGTSKPGMEQPVYYWDPVIAPSGATFYSGTAFADWKGDLLVGSLTPGLLVRLRITNGR
                     VVLEERYLAELRERIRDVREGPDGAIYLLTDGSSNGRLLRVTPAGR"
     gene            complement(3362..4411)
                     /locus_tag="VFX87_09150"
     CDS             complement(3362..4411)
                     /locus_tag="VFX87_09150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995123.1"
                     /translation="MRFGLFFQAPERAGETHAQRFSEMLELATLADSLGFDVAWLAEI
                     HFGAAFSLLSTPLMAVPAIAQRTKRIRVGTAVTLLPLHHPLACAEQAATADILSGGRL
                     EFGVGRGSIPTQFHGFRVPVSENRARFAECLDIIRLAWTKDRFSYDGTFYQIENLSVV
                     PKPLQRPHPPIRVAVHTPESFAHIAELGVPIYSGTTTTPLPLLRECMASYRQKLAAAG
                     HAWGAEQMALMQPVHIGATPATAREAMRPGVMQYYKNLETIFSSLPDSYADHLPRLKH
                     IRETIADLPYDRFCRDQAVFGDAAEVVDRLQRAKEEFSLSQIICWFDQGAMLPRTEVE
                     RTMRTFAEQVMPKLD"
     gene            complement(4425..5267)
                     /locus_tag="VFX87_09155"
     CDS             complement(4425..5267)
                     /locus_tag="VFX87_09155"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995124.1"
                     /translation="MDIVDSQVHLWAAESPARPWPAGRAAEAQKPYQILKETLLFQMD
                     LAGVRRMVLVPPSWEGDRNDMALEAARSYPDRFAVMGRLALQDPTSRALVADWRKQPG
                     MLGMRFTFHNEHYRHLLTDGSADWLWAAAEKAGIPLMVLVPGALDHLDRIATRHPGLK
                     LVIDHVGISVRGKAPKVFEELPAVCALAKHPNVAVKASGMVSLSAQPYPFPDLHDGIR
                     RLVDAFGPKRTFWGTDLTRMPCTYYECITLFTQHLPWLKGEDLDHVMGRGVCEWLGWP
                     LTRV"
     gene            complement(5291..>5562)
                     /locus_tag="VFX87_09160"
     CDS             complement(5291..>5562)
                     /locus_tag="VFX87_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995125.1"
                     /translation="TKNGIYSSGVVPEIETLFERQARELDRKKREGMLHQIQQIMYDR
                     AMHVPVYELAFLWGIGPRVDEACVDHIKGFSYSAPYEDLRLKAPR"
BASE COUNT          783 a         1936 c         2003 g          840 t
ORIGIN      
        1 cccgcgatcg gcacgtccac ggagcggttg tggccgagct gggtcgggtt gatgtccacg
       61 tggatcacct tcaggtcggc cgcgaagcgc gggggccggc cgaactggat cacccagttg
      121 aaccgcgtgc ccaccgccag caccacgtcg gcctcggtga acgccttgct gcgcgcgttc
      181 aggaatgcca gctcgtgatc ctccgcgatg aggccgcggg agatcggcgt ggtgtagaag
      241 gggatgccgg tggcctccac gaaggcctgg agcgcggccg cgccgtcgga ccaccacacg
      301 ccgctgcccg cgatgattac cggtcgctca gctcgcgaca gcacgttgat cgcctcccgg
      361 acggctgacg gatcgcccag gctgcgcggc gccgggcgcc acgggccggg gtagtggagc
      421 tgctcttcct cgatcttctc gcccaggatg tcgcccggca tgtcgatgta gacggggccg
      481 ggccggccgg aggtcgcctg gcggaacgcg gtgtccacca cgtcggggat gcgcctggcg
      541 tcgtagatgc gctcggtcca cttggtgatc ggcttcatca gggcgacctg gtcgatctcc
      601 tggaaggcct ccatgccgag gtagacccgc gggctcgagc cgccgatcgc gatgagcggg
      661 gcgcagtcgg tgaaggcgtt cgccacgccg gtggccaggt tgatggcgcc ggggcccgag
      721 caacccatcg ccacgccggg gcgccgcgtc acccgcgtcc aggcgtgcgc ggccagggcg
      781 gccgcctgct cgtggcgcgt gtcgatggcg cggatgccga gcttgatgca ggccgcctcg
      841 gtctcgagca tgggaccgcc catgagatag aacatcgtgt ccacgccctg cgacctgagc
      901 gcctgcgcca gtacttccga tcccgtgatc gtggccatgg cctgtccttt ccttgctgcc
      961 ggtgagaggg tgtcgagaag atagcagatg tatgatgggc gcgggaggga agcgctcaat
     1021 ggtccacgtc cagttcaacc cgatggatcc cgcgttcgtg gcggacccgt atccgaccta
     1081 ccaccgcctg cgggccgagg atcccgtcca ccacagcccg ctcgggttct gggtgctgac
     1141 gcgctacccg gacgtgatgg ccatgctgcg cgacccgcgg ctcatcaagg agcccatcgc
     1201 ggccttcgtg gccgcgcgct tcggcatggc ggcgccgccg ccgggcctgg gtctctccat
     1261 gctggaccgc gacccgccgg accacacgcg cctgcgcggg ctggtcagca aggcgttcac
     1321 gccgaaggcg ctggagagcc tgcgccccca catccagcag atcgtggacg atctgctggc
     1381 cgacgccgcg gggaagggcc agatggacct gatcgaggag ttcgcgtatc cgctcccggt
     1441 ccgggtgatc tgcgagatgc tgggcgtccc ggtgaaggac cacgagcgct tcaaggcctg
     1501 gggcctcgac atcgcgcgcg gcctcgacgc gatcatgctg ccgccggact ccgaggtggg
     1561 ccagcgcagc gtctccggcc ggcgggccct ggccgagtac ttccgcgcgc tgatcgcgga
     1621 gcggcgcgcc gccccgcgcg acgacatgct ctccgccctg atcgcggccg aggaggccgg
     1681 cgacaagctg aacgaggaag agctgctcgc cacctgcatc ctcctgctgg tcgcgggcca
     1741 cgagaccacc gtgaacctga tcggcaacgg caccctggcg ctactccgcc atcccgatca
     1801 gctccagaag ctgcgcgcga gccccggcct catcgccacc gcggtggagg agttgctgcg
     1861 cttcgacggg cccgtgcagc gcaccgcgcg catcccgagc gaggacatca cgatcggcgg
     1921 ccggacgatc cccaagggcg acatggtgat gccgttcctg ggcgcggccg atcgcgaccc
     1981 cgcccagttc ccggacccgg accgcctcga catcacgcgc gccgacaacc gccacatcgc
     2041 cttcggcatg ggtatccact tctgcctggg cgctccgctc gcgcgcatgg aggggcagat
     2101 cgccatcaat gcgctgctcg cgcgcctgcc gaagctggcc ctggccaccg accggcccca
     2161 gtttcgccag agcctgaccc tgcgcgggct gcaggcgctg ccggtcagtt tttgaggacg
     2221 gcgcgggtcg cggccgtcct gccggcgctc gtcctcctcg tcctgcccgc gctcgcgcag
     2281 gaggcgccgc gctcgccgac gcctgccgcg gtcaagctgg ccgcgacggt caccgacgtg
     2341 gcgcgcgggc tcgagcatcc ctggggcgtc gagctcctgc ccgacgggcg cttcctcgtg
     2401 accgagcgcc cggggcgcct gcgtattgtc gaccgtgatg ggcgcctctc ggtgcccctg
     2461 attggcgtgc ccgaggtcta cgcgcgcggg cagggcggcc tgctcgacgt ggcgctcggc
     2521 cccggcttcg cccaggaccg gctcgtctac ttctcgttct cggagccggg cccgggcggc
     2581 gcgggcaccg cggtggcacg cgggcggctc agcgaacgcg ggctcgaggg cacccaggtg
     2641 atctggcgcc agcagccgaa ggtggagggg gcgaatcact ggggctcgcg cctggtcttc
     2701 cgggccgacg gcacgctctt cgtcacggtc ggcgaccgct atgcctatcg cgaccgcgcg
     2761 caggatctct cgacgacgct cggcaaggtc gtgcgcatca accccgacgg ctcggtgccc
     2821 cgcgacaatc cgttcgtcgg ccgcgccggc gcgctcccgg agatctggtc gctcggccat
     2881 cgcaatgtcc aggcggcggt gctcgacgcc cgtggcgagc tgtggacggt ggagcacggc
     2941 gcgcgcggtg gcgacgagct caacaacccg cagccgggaa agaactacgg ctggccggtc
     3001 atcacctacg gcgtcgacta ctcgggcgcg cgcatcggga tcggcacgtc gaagccgggc
     3061 atggagcagc ccgtctatta ctgggatccg gtgatcgccc cgtccggcgc gaccttctac
     3121 tcgggcaccg cgttcgccga ctggaagggc gacctcctgg tcggctcgct gacgccgggc
     3181 ctgctcgtgc ggctgcggat cacgaacggc cgcgtggtgc tggaggagcg ctacctggcg
     3241 gagctccgcg agcggatccg cgacgtgcgc gagggcccgg acggcgccat ctacctcctg
     3301 accgacggca gctccaacgg ccgcctcctc cgggttaccc cggccggcag gtaggccctg
     3361 gtcagtcgag cttcggcatg acctgctcgg cgaaggtgcg catcgtgcgt tcgacctccg
     3421 tgcgcggcaa catggccccc tggtcgaacc agcagatgat ctgggacagg ctgaactctt
     3481 ccttcgcgcg ctggaggcgg tccaccacct ctgcggcgtc gccgaacacg gcctggtccc
     3541 ggcagaacct gtcgtagggc agatcggcga tcgtctcccg gatgtgcttc agccggggca
     3601 ggtgatcggc gtaggagtcg ggcaggctcg agaagatcgt ctccaggttc ttgtagtact
     3661 gcatcacgcc cggccgcatg gcctcgcgcg ccgtcgccgg cgtggcgccg atgtgcaccg
     3721 gctgcatcag cgccatctgc tcggcgcccc aggcgtgccc ggccgcggcg agcttctgcc
     3781 ggtacgacgc catgcattcg cgcagcagcg gcagcggggt ggtggtcgtg cccgagtaga
     3841 tgggcacgcc cagctccgcg atgtgggcga agctctccgg ggtgtgcacg gccacccgga
     3901 tcggcgggtg cgggcgctgg agcggcttcg gcaccaccga gaggttctcg atctggtaga
     3961 acgtgccgtc gtagctgaag cggtccttcg tccaggccag gcggatgatg tcgaggcact
     4021 cggcgaagcg cgcgcggttc tccgagaccg gcacccggaa gccgtggaac tgggtgggaa
     4081 tggagccgcg gcccaccccg aactcgaggc gcccgcccga gaggatgtcc gcggtggccg
     4141 cctgctccgc gcaggcgagc gggtggtgca gcggcagcag cgtcaccgcg gtgccgaccc
     4201 ggatgcgctt cgtgcgctgg gcgatcgcgg gcaccgccat gagcggggtg gagagcagcg
     4261 agaaggcggc gccgaagtgg atctcggcga gccaggccac gtcgaagccg agcgagtcgg
     4321 cgagcgtggc cagctcgagc atctcgctga agcgctgggc gtgcgtctcg ccggcccgct
     4381 ccggcgcctg gaagaagagg ccgaatcgca tcggaggcgc gcgctcagac gcgcgtgagc
     4441 ggccagccga gccactcgca gacgccgcgc cccatcacgt ggtccagatc ctcgcccttg
     4501 agccacggca ggtgctgggt gaagagcgtg atgcactcgt agtaagtgca cggcatgcgg
     4561 gtgaggtcgg tgccccagaa cgtccgcttc gggccgaagg cgtccaccag ccgccggatg
     4621 ccgtcgtgca gatccgggaa cgggtagggc tgggccgaga gcgacaccat gcccgaggcc
     4681 ttcaccgcca cgttcgggtg cttcgccagc gcgcagaccg cggggagctc ctcgaagacc
     4741 ttgggcgcct tgccgcgcac gctgatgccc acgtgatcga tcacgagctt gaggccgggg
     4801 tgccgggtcg cgatgcgatc gaggtggtcg agcgcgcccg gcaccaggac catcagcggg
     4861 atgcccgcct tctcggcggc ggcccagagc cagtcggcgg agccgtcggt gagcaggtgg
     4921 cggtaatgct cgttgtggaa ggtgaagcgc ataccgagca tgccgggctg cttcctccag
     4981 tcggccacca gcgcgcggct cgtcgggtct tgcagcgcga gccggcccat gaccgcgaac
     5041 cggtcggggt agctgcgcgc cgcctccagc gccatgtcgt tgcgatcgcc ttcccaggag
     5101 ggcggcacca ggaccatgcg gcgcaccccg gcgagatcca tctggaagag cagcgtctcc
     5161 ttcaggatct ggtagggctt ctgcgcttcc gccgcgcggc cggccggcca cggccgggcg
     5221 gggctctccg cggcccagag atgcacctgc gagtcgacga tgtccatgag gatctcctgt
     5281 gggagcggag ttaccgcggg gccttgagcc tcagatcctc gtatggcgcg gagtacgaga
     5341 agcccttgat gtggtccacg cacgcctcgt ccacccgggg accgatgccc cagaggaagg
     5401 cgagctcgta gacgggcacg tgcatcgcgc ggtcgtacat gatctgctgg atctggtgca
     5461 gcatgccctc gcgcttcttg cggtcgagct cgcgggcctg ccgctcgaag agcgtctcga
     5521 tctccgggac caccccggac gagtagatgc cgttcttggt ga
//
LOCUS       DASUFA010000246         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1642665, whole genome shotgun sequence.
ACCESSION   DASUFA010000246 DASUFA010000000
VERSION     DASUFA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642665"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VFX87_09165"
     CDS             <1..1189
                     /locus_tag="VFX87_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX4995126.1"
                     /translation="ADNVERLGGHVHWATTAEEAREMVLRLCRDRGVRMAVKSKSMAT
                     EEIDLNEALEHAGVTPVETDLGEYIIQLAHEKPSHIIAPAIHKTKGQVAELFSRELRG
                     EFPADPEVLTAVARKALREKFLQADMGITGANFAVADTGTVVLVTNEGNGRMVTSLPR
                     IHVALMGVEKVIPSMTDLVVFLAILARSATGQKLSSYTTLVRGPRRAGELEGPEEFHL
                     ILLDNGRSRQIASTLREALYCLRCGACLNVCPVYRQIGGHAYGYTYPGPIGILLTAML
                     KGNESVRDLAHASSLCGACKDVCPVRIDIPRMLVELREHLDREKIAPWTERVVFKALR
                     RVLLSPTAYRLSATVGRWVQRPFIRHGRLRGLPLVFGKWTKTRDLPPLAARSFRERWK
                     ELR"
     gene            1186..1929
                     /locus_tag="VFX87_09170"
     CDS             1186..1929
                     /locus_tag="VFX87_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEX4995127.1"
                     /translation="MTGRAEFIERIRREVSKTPGLFGASTAPRPADPVMAAETVRREL
                     TERWPEALERFRLEFERVAGVFHRVPDLAAVPAAIGAIARAYGAKSLVTWDPAALGFD
                     AAPALTREGLEVVAARTGEEDEAARLRHREQAARAQVGVTGADFVLGETGTLILLSGS
                     GRPRSTSLLPDVHVAVFGRDRLLETLEQMGIMLEALHVDPDHAMSGAMSGAMINFITG
                     PSRTADIELTLTRGVHGPKEVHAIFVEAP"
     gene            1926..2345
                     /locus_tag="VFX87_09175"
     CDS             1926..2345
                     /locus_tag="VFX87_09175"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX4995128.1"
                     /translation="MSEQRLFTPDEVNRLIPRLTELMETAIERHGQALALQERLRDDR
                     ARVSASGGERRDAMDWKARAERLDGLGIEVRQILQDVQAMGGVVKDLETGLVDFPGRG
                     PAGPGGPVVNLCWKLGEDAVRFWHGMDEGFANRKPLP"
BASE COUNT          359 a          840 c          917 g          350 t
ORIGIN      
        1 cgccgacaac gtcgagcggc tgggcgggca cgtgcactgg gccaccaccg ccgaggaggc
       61 gcgcgagatg gtcctgcgcc tctgccggga ccggggcgtg cggatggcgg tgaagtcgaa
      121 gtccatggcc accgaggaga ttgacctcaa cgaggcgctc gagcacgccg gggtcacgcc
      181 ggtcgagacc gatctgggcg agtacatcat ccagctcgcc cacgagaagc cgtcgcatat
      241 catcgccccc gccatccaca agaccaaggg gcaggtggcc gagctcttct cgcgggagct
      301 gcgcggggag tttcccgccg acccagaggt cctgaccgcg gtggcgcgaa aggccttgcg
      361 cgagaagttt ctccaggccg atatggggat caccggcgcc aacttcgcgg tggccgatac
      421 cggcaccgtg gtcctcgtca ccaacgaggg caatgggcgg atggtgacgt cgctgccgcg
      481 gattcacgtg gccctcatgg gcgtggagaa ggtgatcccg tccatgaccg acctcgtggt
      541 gttcctggcc atcctggccc ggagcgcgac gggccagaag ctgtcctcct acacgacgct
      601 ggtgcgcggg ccgcggcggg cgggggagct cgaggggccg gaggagttcc acctgatcct
      661 cctcgacaac ggccgctcgc gccagatcgc ctcgactctc cgggaagccc tctattgcct
      721 tcggtgcggc gcctgcctca acgtctgccc ggtgtatcgc cagatcggag gccacgccta
      781 cggctacacg tacccggggc ccatcggcat cctcctcacc gcgatgctca agggcaacga
      841 gtcggtgcgc gatctggctc acgcctcgtc gctctgcggg gcgtgcaagg acgtgtgccc
      901 ggtgcggatc gacatcccgc gcatgctggt cgagctgcgc gagcatctcg atcgggagaa
      961 gatcgcgccg tggacggagc gggtggtgtt caaggccctc cggcgtgtgc tcctgtcgcc
     1021 gaccgcctac cggctctccg ccacggtcgg acggtgggtc caacgaccat tcatccgcca
     1081 cggccggctg cgcggcctgc cgctggtctt cggcaagtgg acgaagaccc gcgatctccc
     1141 gccgctggcg gcccggagct tccgggagcg gtggaaggag ctgcggtgac gggccgcgcc
     1201 gagttcatcg agcggatccg ccgcgaggtc tcgaagaccc cgggcctctt cggcgcctcg
     1261 accgcgccgc gccccgccga tccggtgatg gcggcggaga cggtccggcg cgagctgacc
     1321 gagcggtggc cggaggcgct cgagcggttc cgcctggagt tcgagcgcgt cgcgggcgtg
     1381 ttccatcgcg tcccggacct ggcggcggtg cccgcggcaa tcggcgccat cgcccgcgcc
     1441 tacggcgcga agagcctggt cacctgggac cccgccgccc tcggcttcga cgcggcgccc
     1501 gcgctcacgc gcgagggcct ggaggtcgtc gcggcgcgca cgggggagga ggacgaggcg
     1561 gcccgtctcc gccaccgcga gcaggcggcc cgggcccagg tcggcgtcac cggggccgac
     1621 ttcgtgctcg gggagaccgg gacgctgatc ctgctctccg gatcggggcg gccgcgctcg
     1681 acctcgctgc tgcccgacgt gcacgtggcg gtcttcggcc gcgaccggct gctcgagacg
     1741 ctggagcaga tgggcatcat gctcgaggcg ctccacgtgg atcccgacca tgcgatgtcg
     1801 ggggccatgt cgggcgcgat gatcaacttc atcaccgggc cctcgcggac cgccgacatc
     1861 gagctcaccc tgacccgggg cgtgcacggg cccaaggaag tgcacgcgat cttcgtggag
     1921 gcgccgtgag cgagcagcgg ctcttcaccc cggacgaggt caatcgcctg atcccgcggc
     1981 tcaccgagct gatggagacc gccatcgagc gccacggcca ggccctggcg ctgcaggagc
     2041 gcctgcgaga cgatcgcgcg cgggtatcgg cctccggcgg cgagcggcgc gacgcgatgg
     2101 actggaaggc ccgcgcggag cgtctcgacg ggctcggaat cgaggtgcgc cagatcctgc
     2161 aggacgtcca ggccatgggc ggcgtcgtga aggatctcga gacgggcctg gtggactttc
     2221 ccggccgcgg gcccgccggg ccgggcgggc cggtcgtgaa tctctgctgg aagctcgggg
     2281 aggacgcggt gcggttctgg cacgggatgg acgagggctt cgccaatcgg aagccgctcc
     2341 catgattggc gaggtgcagc cgacggcgag ccgagcggat acatcgattg gcgaggtgca
     2401 gccgagggcg agccgagcgg atacatcgat tggcgaggtg cagccgaagg cgagccgagc
     2461 ggatgc
//
LOCUS       DASUFA010000247        11721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1095425, whole genome shotgun sequence.
ACCESSION   DASUFA010000247 DASUFA010000000
VERSION     DASUFA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095425"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFX87_09180"
     CDS             <1..230
                     /locus_tag="VFX87_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995129.1"
                     /translation="QLVEELGGQVIGIGALWRRTKKSVIDGKEIFSLVSRDFPTYTTE
                     TCPLCKKGVPLNQEYVKRREHRKPSSAHEKT"
     gene            477..1352
                     /locus_tag="VFX87_09185"
     CDS             477..1352
                     /locus_tag="VFX87_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4995130.1"
                     /translation="MTHRDAYLLLFVVALVWAGNFPLAKLGLSELGPLTLAALRALIA
                     APLLVLASRVLEGPLPPLARRDLTAVIVLGVSGFVGNSTLWYTGMMFTTPANAGILGA
                     AAPVVVALAASAMLRERLSVLNLAGIALTMGAVVLTIARGSFEVLLTLSANRGDVIIL
                     ASQMLWVTYTLYSRANRSTFSPLQMLAGAHVVAAGLLLPLALLERPWQSFAGASWVAV
                     VVVLYSSLLGTPAHLAFYQAVRTVGPGRAAVFTNLIPFLVLGLSWLMVGEPIRWYHWI
                     GACGVIAGVALTTRR"
     gene            complement(1357..1863)
                     /gene="nrdR"
                     /locus_tag="VFX87_09190"
     CDS             complement(1357..1863)
                     /gene="nrdR"
                     /locus_tag="VFX87_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX4995131.1"
                     /translation="MKCPFCGHTEDRVVDSRVGRDGEFIRRRRACLKCNRRYTTYEYI
                     EDVLPHVVKRDGRREPFDRQKLRGSILKSCEKRSVGVQAVDDVVADIEAKLHDRAEKE
                     ITSLELGEIVMEHLQKLDQVAYVRFASVYRQFKDISQFMDEVKHLIKDDKPAKPVAAK
                     APKATAKR"
     gene            complement(1860..3107)
                     /gene="glyA"
                     /locus_tag="VFX87_09195"
     CDS             complement(1860..3107)
                     /gene="glyA"
                     /locus_tag="VFX87_09195"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX4995132.1"
                     /translation="MSHLEQVDPEIAKAIRAETERQSRNLELIASENFVSEAVLEAVG
                     SVMMNKYAEGYPGKRYYGGCEIVDIAENLAISRAKELFGAEHVNVQPHSGSQANMAVY
                     FTLLKPGDTVLGPNLAHGGHLTAGSPMNFSGRFYNIVPYGVTKGTERIDMDQVRDLAR
                     AHRPKLIIAGGSAFPRAIEFKPFREIADEVEAKLMADIAHPAGLVAAGLHPSPVPFAD
                     FVTTTTHKTLRGPRGGMVMCRGQYAKDVDRTVLPGIQGGPLMHVIAAKAVAFREAQTQ
                     EWRAYQAQIVKNAKAFAEAMLARDFRLVSGGTDTHLLLVDLTSRNVTGKDAQEMLDRA
                     WITVNKNGIPFDTKGPMITSGIRIGTPAVTTRGMKEPEMARIAELIDRVLSRPGDGGV
                     EATVRSQVQELTSQFPLYPERLK"
     gene            complement(3104..3568)
                     /gene="rpiB"
                     /locus_tag="VFX87_09200"
     CDS             complement(3104..3568)
                     /gene="rpiB"
                     /locus_tag="VFX87_09200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX4995133.1"
                     /translation="MVSTIALGADHAGYELKEALKGWLINHGFHVLDLGTHSTESVDY
                     PDYAALVGESVADHKVERGLLVCGTGIGMAMAANKVPGVRAALCGDLYTARMSREHND
                     ANVLVLGGRLMGADMAADILRAWLETDFAGGRHARRVAKIADIQRRHPEGRP"
     gene            complement(3673..4890)
                     /locus_tag="VFX87_09205"
     CDS             complement(3673..4890)
                     /locus_tag="VFX87_09205"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995134.1"
                     /translation="MNVRAIVAWTLYDFANSAFAAVIFATIYAAYYALAVVGNDGGAG
                     DLWWGRVVSVSMGLVAVTSPFLGGVADRAGIRRPLFIGFTAVAVAATALMATVEPGMV
                     VWGFVLGVIGNFAYESALVYYNAYLQDLAPASHQGRVSGWGFAVGYAGSIAALLAALP
                     FVRSRNYEGAFFATAALYAVFSLPAFFMLPALPSGRVSVLRAAREGASQVAATARRIL
                     ALPDLRRFLGAYFIYEDGVNTVIAFSGIFAAQTLGFPMERLIALYIVVQISALVGALA
                     WARPTDVLGPRRVVMITLCQWSAVVVAAYFVETQGQFWALAVVAGTGLGAVQAASRAF
                     LARLAPPGMEAELFGFYSLCGKSAAVMGPLVFGGISHTAGGNQRAGILAIGSFFVIGL
                     ALLSRVKSGGRAR"
     gene            complement(4887..6188)
                     /locus_tag="VFX87_09210"
     CDS             complement(4887..6188)
                     /locus_tag="VFX87_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4995135.1"
                     /translation="MDVIGAERIPRAGPLIVAANHHNALVDGVLLLATLPRRLVSVAK
                     APLFSNPLIGPFLRLIGALPVHRRQEGGTDPARNDRLFAASTKALRDGDAILIFPEGV
                     SQPEPTLMPLRTGAARMLLAAEADSVGPVTLLPVGLMFHEPGTFRTGWALIAMGEPVE
                     TADHVALARSQPEEAARRLTARLEEALRGLIAEVDDRETLRLVEHAERLWREERGGGA
                     PRDAAASADWRRRATRAYRYLLHHDPAGIEILRGLVDRYVKDVEAASLGTAVGLTPPG
                     ARVVLRYAVRQLAILVGGLPLALWGLASHAVPYWLTWIAAKLLRPEPDVEATYKLVAG
                     VVMYPLAWIAEGWLLWRWGGGWPLALFVVLLIPGAFFALAWSERLRRLYREGRALIRL
                     LVDRDLGRHLGGRRRAILDEMERAVARVPEPVLAGREESRG"
     gene            complement(6266..7648)
                     /locus_tag="VFX87_09215"
     CDS             complement(6266..7648)
                     /locus_tag="VFX87_09215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4995136.1"
                     /translation="MTTTLTFLGAAGGTVTGSKHLLENGDRRVLLDCGLFQGVKELRE
                     RNWAAPPVDPRTLGAVILSHAHIDHSGYLPRLCRDGFAGPIYCTPGTSDLLQVMLPDA
                     AHLQEEEADFANRHRTSRHDPALPLFTTADAERALAQVTRVDFGRRFTATRGIEARFT
                     RSGHILGAGLVTCEIGGRRLVFTGDLGRYDVPIMVDPEPVAEADVLLVESTYGDRVHP
                     PDDPTERLIAAVRRAIDQKGWLLIPAFAVGRSQEILYDLRRLEAEGRIPGLPVYLDSP
                     MAIQATVVYAKHPEEHDTELRRASTGGARPFAPRRFHLSQTAEESKRLNDTAGPGIIV
                     AGSGMATGGRILHHLKRLLPDPRTTVLFVGYQAAGTRGRLLRDGAREIRMLGMMVPVH
                     ATIMVSDAYSAHADREEILRWLGGFTRPPGMTYVVHGEAGAAEALRDAIVSRLGWNAA
                     VAQDGQRVTL"
     gene            complement(7645..8886)
                     /gene="thiI"
                     /locus_tag="VFX87_09220"
     CDS             complement(7645..8886)
                     /gene="thiI"
                     /locus_tag="VFX87_09220"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEX4995137.1"
                     /translation="MTGAAPAAIVHYHEISLKRGNRPLFLRQLQQNLLRAIEDLGPVE
                     LVQLPGRIMLDLSRHPAPRLLPERLARVAGVANFALAIRTGVSREALRAAVEEAIAGR
                     VFGSFRITARRAFKTFPMTSVELNRDLGGHVLALRPDVRVSLEHPELNVRVEVLPDEA
                     FVYCDPQPGAGGLPVGTGGTVAALLSGGIDSPVAAWRLMRRGCRVVFVHFHSAPYLPD
                     LSRGKARELVARLTQWQYHSRLFIVPFGEIQREVVLSVPPPFRVVVYRRLMVRIAERL
                     ARHAGALALVTGESLGQVASQTLHNLTRIDEVAGMPILRPLIGMDKLEITEQARALGT
                     YEISIEPDADCCTLFTPRHPATRIEAEAIAALEARLDLDRLVTQGVEAAVQEQFEFPA
                     GAGPYPPRGAARRPLTEPVSE"
     gene            complement(8886..9014)
                     /locus_tag="VFX87_09225"
     CDS             complement(8886..9014)
                     /locus_tag="VFX87_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995138.1"
                     /translation="MKRPKPIHAKVRKPMPAKPPKVEVPKTVYRRRPKHRRPAGSE"
     gene            complement(9042..9866)
                     /locus_tag="VFX87_09230"
     CDS             complement(9042..9866)
                     /locus_tag="VFX87_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995139.1"
                     /translation="MDARVEGLGLGSIQDINALPREVAEALYARLVPEELLDRFAIDP
                     LTLCDKAGARLVRVTAPADQPWARVEVRSSPEDRDPALLVDVEMSPLSVPELAFVQIT
                     DPAGARYAIDRDPDGRDTLFGTTCRNIEEERRAMRGGLAPGQVRRGLRLLSRVLDAME
                     GFCALIGKEIYLIEPLFYHSAILYERRGCGYLMGRDVMESIHAGFSDGGLLVRALDAS
                     SDFRAPEAARSVRGRSWAIHDGVGDGLWAGGAVGGVKMYKVAGRQAGVNTFPGAIY"
     gene            complement(9878..10501)
                     /locus_tag="VFX87_09235"
     CDS             complement(9878..10501)
                     /locus_tag="VFX87_09235"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX4995140.1"
                     /translation="MRITGVIQAGGRSTRMGGQPKALLELGGRRIIDRVAHVVRQVAD
                     DLLIVTNTPELYASLGVPMVPDAFPDHGSLGGIYSGLQAASGDAAFTVACDMPFLMPE
                     VARLVTERAGEADVVVPRVGEQWETLHACYAKACLAPIETRLRAGRLKIIGFYDDVRV
                     LEIPAREIARYRAPEVVFMNVNTPEELEAARVLLPALEPGAAAAGPC"
     gene            complement(10492..11133)
                     /locus_tag="VFX87_09240"
     CDS             complement(10492..11133)
                     /locus_tag="VFX87_09240"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX4995141.1"
                     /translation="MSAPTISTRVVSVDPVQPDRAVMRAAGALVEAGGLVAFPTESFY
                     GLGADALDPAAIARVFEVKGRPDDKPLLVLVDSIDMVTELAAAIPDGARALMARHWPG
                     ALTLVLRAAARVPAGLTGGTGTVGVRLPGHAVARALVAAAARPVTAPSANPSAAPPPR
                     TAEDVRGYFDGRVELILDGGTTLGGAGSTVADCTVWPPRILRQGPVIVEAPCG"
     gene            complement(11130..>11721)
                     /locus_tag="VFX87_09245"
     CDS             complement(11130..>11721)
                     /locus_tag="VFX87_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX4995142.1"
                     /translation="AYSPAPVMSDAMQDRVMAEIVRPVIAAMGKEGAPYTGVLFVGLM
                     ITREGPKVIEFNCRFGDPECQAIMPRLDGDLLALLQAAATGQGLPATLSWSARSSVCV
                     VMTSAGYPGRYETGRPITGVAAAAALPGINVFHAGTAMAGDALVTAGGRVLGVQALGV
                     DVASAIRAAYAGVERIRFDGAHYRRDIGHHALRRIG"
BASE COUNT         1695 a         4228 c         4210 g         1588 t
ORIGIN      
        1 cccagctcgt ggaggagctg ggcggccagg tgatcggcat cggcgccctg tggcgccgca
       61 ccaagaagtc ggtgatcgac ggcaaggaga tcttcagcct ggtgagccgc gacttcccca
      121 catacaccac cgagacctgc ccgctctgca agaagggcgt gccgctcaac caggagtacg
      181 tgaagcggcg cgagcatcgc aagccgtctt ccgcgcacga gaagacctag ccggctcgcc
      241 cccaagatgt gggggctgca gcgcgttacg acccctccat ccggtatttt gtccttgaca
      301 ccctctggac cctcccctta gagtccggac agatcgaccg ccggacgcgc ggggatcggg
      361 aggggggtga cacgatggcg aagaagaagg ccgcgaagaa gaaaaagaag aagtaaggcg
      421 ccattcgtgg cgctcggccc tgtcggcccc atcccggcag ggccgttcca tctcacgtga
      481 cccatcgcga cgcctacctc ctcctgttcg tcgtggccct cgtctgggcc gggaacttcc
      541 ccctcgccaa gctcggcctc tccgagctcg gcccgctcac gctcgcggcc ctgcgcgcgc
      601 tgatcgccgc gcccctgctc gtgctcgcct cccgggtgct cgagggcccg ctgccgcccc
      661 tcgcccgccg cgacctgacc gcggtgatcg tgctcggcgt ctccgggttc gtgggcaact
      721 ccacgctctg gtacaccggc atgatgttca cgacccccgc caacgcgggg atcctcgggg
      781 cggcggcgcc ggtggtggtg gcgctcgcgg cctccgcgat gctgcgcgag cgcctctcgg
      841 tcctcaacct ggccgggatc gcgctcacga tgggcgcggt ggtgctcacc atcgcgcggg
      901 ggtcgttcga ggtgctgctg acgctgtcgg ccaaccgggg cgacgtgatc atcctggcct
      961 cccagatgct ctgggtgacc tacacgctct acagccgcgc caaccgctcc accttctcgc
     1021 cgctccagat gctggcgggc gcccacgtgg tggcggcagg cctgctgctc ccgctggccc
     1081 tcctggagcg gccctggcag agcttcgcgg gggcctcgtg ggtggcggtg gtggtcgtgc
     1141 tctacagctc cttgctgggc acaccggcgc acctcgcctt ctaccaggcg gtgcggaccg
     1201 tggggccggg acgggccgcc gtgttcacga atctcatccc gttcctggtg ctgggactct
     1261 cctggctgat ggtgggcgag ccgatccgct ggtaccactg gatcggcgcc tgcggcgtga
     1321 tcgccggcgt ggcgctgacg accagacgct gaggggttac cgcttggccg tggccttcgg
     1381 cgccttggcg gccaccggct tggccggctt gtcgtccttg atgaggtgct tcacctcgtc
     1441 catgaactgg gagatgtcct tgaactggcg gtacaccgag gcgaaccgca cgtaggccac
     1501 ctggtccagc ttctggagat gctccatcac gatctcgccc agctcgaggc tcgtgatctc
     1561 cttctcggcg cggtcgtgca gcttggcctc gatgtcggcg accacgtcgt ccaccgcctg
     1621 cacgcccacc gagcgcttct cgcaggactt gaggatggag ccgcgcagct tctggcggtc
     1681 gaagggctcg cggcggccgt cgcgcttgac cacgtggggc agcacgtcct cgatgtactc
     1741 gtaggtcgtg tagcggcgat tgcacttgag gcacgcgcgg cgccggcgga tgaactcccc
     1801 gtcccggccc acccgcgagt ccaccacgcg gtcctcggtg tggccgcaga acgggcactt
     1861 cacttgagac gctccggata gagcgggaac tgcgaggtca gctcctggac ctggctgcgc
     1921 accgtcgctt ccaccccgcc gtctcccggg cgggagagca cacggtcgat cagctccgcg
     1981 atccgggcca tctcgggctc cttcatgccg cgggtggtca ccgcgggcgt gccgatgcga
     2041 atgccgctcg tgatcatcgg ccccttggtg tcgaagggaa tgccgttctt gttgaccgtg
     2101 atccaggcgc ggtcgagcat ctcctgcgcg tccttgccgg tgacgttgcg cgaggtgaga
     2161 tccaccagga gcaggtgcgt gtcggtgccg cccgagacca gccggaagtc gcgggcgagc
     2221 atcgcctccg cgaacgcctt ggcgttcttc acgatctgcg cctggtacgc gcgccactcc
     2281 tgggtctggg cctcgcggaa ggccaccgcc ttggccgcga tcacgtgcat caggggcccg
     2341 ccctggatgc caggcagcac ggtgcggtcc acatccttcg cgtactggcc gcggcacatc
     2401 accatgccgc cccggggccc gcgcagggtc ttgtgggtgg tggtcgtgac gaagtcggcg
     2461 aagggcaccg gggagggatg caggcccgcc gcgaccaggc ccgccgggtg ggcgatgtcg
     2521 gccatcagct tggcctcgac ctcgtcggcg atctcgcgga acggcttgaa ctcgatggcg
     2581 cgcgggaacg cggagccgcc cgcgatgatg agcttcggcc ggtgcgcgcg agccaggtcg
     2641 cgcacctgat ccatgtcaat gcgctcggtg cccttggtca caccgtaggg gacgatgttg
     2701 tagaagcggc ccgagaagtt catcgggctc cccgcggtga gatgcccccc gtgcgccagg
     2761 ttcgggccca gcacggtgtc ccccggcttg agcagggtga agtagaccgc catgttggcc
     2821 tgggatcccg agtgcggctg cacgttcacg tgctccgccc cgaagagctc cttcgcgcgg
     2881 gagatcgcca ggttctcggc gatgtccacg atctcgcagc cgccgtagta gcgcttgccc
     2941 gggtagccct cggcgtactt gttcatcatc accgagccca ccgcctccag caccgcctcg
     3001 gagacgaagt tctcggacgc gattagctcg aggttgcggg actggcgctc ggtctcggcc
     3061 cggatggcct tggcgatctc ggggtccacc tgctcgaggt ggctcacggc cgcccctccg
     3121 gatgccgccg ctggatgtcg gcgatcttcg ccacccggcg cgcgtggcgg ccacccgcga
     3181 agtccgtctc gagccacgcg cggaggatgt ccgcggccat gtcggcgccc attagccggc
     3241 cgcccagcac gagcacgttg gcgtcgttgt gctcgcggct catccgcgcg gtgtagaggt
     3301 ctccgcagag ggccgcgcgg acgcccggga ccttgttggc ggccatggcc atgccgatgc
     3361 cggtgccgca gaccaggagg ccacgctcga ccttgtggtc ggccacggat tcgcccacga
     3421 gcgccgcgta gtcgggatag tccaccgact ccgtggagtg ggtgccgagg tcgaggacgt
     3481 ggaagccgtg gtttatgagc cagcccttga gggcttcctt tagctcatac ccggcgtgat
     3541 cggctccgag ggcaatcgtg gaaaccacgc gccaacctta cgctccgtcg gggttgagtg
     3601 tcaagacgac gctaccggtg gtatccgccc ggaacgcatc tcttgtccgc tcggctcagc
     3661 cccgggaggg cctcacctcg cacggccacc gctcttcacc cgcgagagca gcgccagccc
     3721 gatcacgaag aaggagccga tggccaggat gccggcccgc tggttgcccc ccgccgtgtg
     3781 ggaaatgccc ccaaagacga gcgggcccat gaccgccgcg ctcttgccgc agagcgagta
     3841 gaagccgaac agctcggcct ccatacccgg gggcgccagg cgcgccagga acgcgcggct
     3901 cgccgcctgg accgccccga gcccggtgcc cgccaccacc gcgagcgccc agaactggcc
     3961 ctgggtctcg acgaagtagg cggcgaccac gacggccgac cactggcaga gggtgatcat
     4021 caccacccgg cgcgggccca ggacgtcggt cggccgtgcc caggccagcg cgccgacgag
     4081 ggccgagatc tgtacgacga tgtagagcgc gatgagccgc tccatcggga acccgagggt
     4141 ctgggcggcg aagatgcccg agaaggcgat gaccgtgttc accccgtcct cgtagatgaa
     4201 gtaggcgccc aggaagcgac gcaggtccgg cagggccagg atccggcgcg cggtggccgc
     4261 cacctgcgag gcgccttcgc gcgcggcccg cagcaccgac acccggccgc tcggaagtgc
     4321 cgggagcatg aagaaggcgg ggagcgagaa gaccgcgtag agcgcggccg tcgcgaagaa
     4381 ggccccctcg tagttccgcg agcgcacgaa gggcagcgcg gccagcagcg cagcgatcga
     4441 gcccgcgtag ccgaccgcga agccccagcc cgagacgcgg ccctgatggc tcgccggagc
     4501 caggtcctgc agataggcgt tgtagtagac gagcgcgctc tcgtaggcga agttgccgat
     4561 cacgcccagc acgaagcccc acaccaccat cccgggctcc accgtggcca tcaaggcagt
     4621 ggcggccacc gccaccgccg tgaagccgat gaagagcggc cggcgaatgc ccgcgcgatc
     4681 ggccacgccg cccaggaagg gcgaggtcac cgccaccagc cccatggaga cggagaccac
     4741 gcgtccccac cagaggtccc ccgccccgcc gtcgttgccg acgaccgcga gggcgtagta
     4801 cgcggcatag atggtggcga agatcaccgc cgcgaacgcg gagttcgcga agtcgtagag
     4861 cgtccaggcc acgatcgcgc gcacgttcat ccgcgggact cctcgcggcc cgccagcacc
     4921 ggctcgggga cccgcgccac cgcccgctcc atctcatcca ggatggcccg ccggcgcccg
     4981 cccaggtgcc gcccgaggtc gcggtcgacc aggaggcgga tcagcgcgcg gccctcgcga
     5041 tagaggcggc gcagccgctc gctccacgcg agcgcaaaga aggcgccggg gatcagcagg
     5101 acgacgaaga gcgcgagcgg ccacccgccg ccccagcgcc agagcagcca gccttcggcg
     5161 atccacgcga gcggatacat gacgacgccc gccacgagct tgtaggtggc ctccacgtcc
     5221 ggctcgggcc gcaggagctt cgccgcgatc caggtgagcc agtacggcac cgcgtgactg
     5281 gccaatcccc agagcgcgag cggcaggcca cccaccagga tcgcgagctg gcgcaccgca
     5341 taacgcagca ccacacgcgc gcccggcggc gtcaacccga ccgcggtgcc gagcgaggcg
     5401 gcctccacgt ccttgacgta gcggtccacc agcccccgga ggatctcgat cccggccggg
     5461 tcgtgatgga ggaggtagcg ataggcccgg gtggcccggc ggcgccagtc ggcgctcgcc
     5521 gccgcgtccc gcggcgcgcc cccaccccgc tcctcgcgcc agagccgctc cgcgtgctcg
     5581 accagccgga gcgtctcgcg atcgtccacc tccgcgatga ggccgcgcag cgcctcctcg
     5641 agccgggcgg tgagccggcg cgccgcctcc tcgggctggc tccgcgcgag cgccacgtga
     5701 tccgcagtct ccaccggctc gcccatcgcg atcagggccc agccggtgcg gaaggtgccc
     5761 ggctcgtgga acatgaggcc gaccggcagg agcgtcacgg gcccgaccga gtcggcctcg
     5821 gcggccagca gcatgcgcgc ggcgccggtg cggagcggca tcagcgtcgg ctcgggctgg
     5881 ctcacgccct cggggaagat caggatggca tccccgtcgc ggagggcctt cgtagaggcg
     5941 gcgaagagcc gatcgttccg cgccggatcg gtgccgcctt cctgccggcg gtgaaccggc
     6001 agggcgccga tcagacgcag gaacggtccg atcagcggat tggagaagag cggcgccttg
     6061 gccaccgaga cgaggcggcg cggcagcgtg gccagcagca gcacgccgtc aacgagcgcg
     6121 ttgtggtggt tggcggcgac gatgagcggg cccgcgcgcg ggatgcgctc ggcgccgatc
     6181 acgtccaccc ggcgataaaa gacggcggcc aggagtcggg ccagggcccg gaccgcgcga
     6241 tacgcccagg acggagggcg cccgctcaga gcgtcacgcg ctggccgtcc tgggcgacgg
     6301 ccgcgttcca gccgaggcgg ctcacgatcg cgtcccgcag cgcttccgcc gcccccgcct
     6361 cgccgtggac cacataggtc atgccgggcg gccgcgtgaa cccgccgagc cagcgcagga
     6421 tctcctcgcg gtcggcgtgg gcggagtagg cgtcgctcac catgatggtg gcgtgcaccg
     6481 ggaccatcat gccgagcatc cggatctcgc gggcgccgtc cctcagcagg cggccccgcg
     6541 tgcccgccgc ctggtagccg acgaagagca cggtggtcct cggatcgggc agcaggcgct
     6601 tgaggtgatg gaggatgcgg ccgccggtgg ccatcccgct gcccgccacg atgatgccgg
     6661 ggcccgcggt gtcgttcagg cgcttggact cctccgccgt ttgcgagagg tggaagcgcc
     6721 gcggagcgaa ggggcgcgcg ccgccggtcg aggcccgccg cagctccgta tcgtgctcct
     6781 cggggtgctt ggcgtagacc acggtcgcct ggatcgccat cgggctatcc aggtagacgg
     6841 gcaggcccgg gatccgcccc tccgcctcca gccggcgcag gtcgtagagg atctcctggg
     6901 accgtccgac cgcgaaggcc gggatcagca gccagccctt ctggtcgatc gcccgtcgga
     6961 ccgccgcgat caaccgctcg gtcggatcgt cggggggatg cacccggtcg ccgtaggtgg
     7021 actccaccag cagcacgtcg gcctccgcca ccggctcggg atccaccatg atcggcacgt
     7081 cgtagcggcc gagatcgccg gtgaagacga ggcggcggcc gccgatctcg caggtgacca
     7141 gccccgcacc caggatgtgc cccgaccggg tgaagcgcgc ctcgatcccg cgcgtggcgg
     7201 tgaaccggcg gccgaagtcc acgcgcgtca cctgggcgag cgcgcgctcg gcatccgcgg
     7261 tcgtgaacag cggcagcgcc gggtcgtgcc ggctggtgcg gtgccggttg gcgaagtcgg
     7321 cctcctcttc ctggagatgg gccgcgtccg gcagcatgac ctggagcaga tcggacgtgc
     7381 ccggcgtgca gtagatcggc ccggcgaagc cgtcgcggca gagccggggc aggtagcccg
     7441 agtgatcgat gtgggcatgg ctcaggatca ccgcgccgag cgtgcgcggg tccacgggcg
     7501 gcgccgccca gttgcgctcg cgcagctcct tcacgccctg gaagaggccg cagtcgagga
     7561 ggacgcggcg gtctccgttc tcgagcaggt gcttggagcc ggtgaccgtg ccgccggcgg
     7621 cgccgaggaa ggtcagggtc gtggtcactc ggagacgggc tccgtcaggg gccggcgcgc
     7681 cgcgccgcgc ggcgggtacg ggcccgcccc ggcggggaac tcgaactgct cctgtacggc
     7741 ggcctccacg ccctgggtca cgaggcggtc cagatccagg cgcgcctcca gcgccgcgat
     7801 cgcttccgcc tcgatccggg tggcggggtg ccgcggcgtg aagagcgtgc agcagtccgc
     7861 gtccggctcg atggagatct cgtaggtccc gagcgcgcgg gcctgctcgg tgatctcgag
     7921 cttgtccatg ccgatgaggg gccggaggat cggcatcccg gccacctcgt cgatgcgcgt
     7981 gaggttgtgg agcgtctgcg aggccacctg ccccaagctc tccccggtca cgagggcgag
     8041 ggcgcccgcg tgacgggcca ggcgctccgc gatgcgcacc atcaggcggc ggtagaccac
     8101 gacgcggaag ggcggcggca ccgacagcac gacttcccgc tggatctcgc cgaagggcac
     8161 gatgaagaga cgcgagtggt actgccactg ggtcagccgc gccaccagct cgcgggcctt
     8221 gccgcgggag agatccggca ggtacggcgc gctgtggaag tgcacgaaga ccacgcggca
     8281 gccgcgccgc atgagccgcc acgcggccac cggcgagtcg atgccgcccg agagcagggc
     8341 ggccaccgtc ccgcccgtgc ccaccggcag cccgcccgcg ccgggctgcg ggtcgcagta
     8401 gacgaaggcc tcgtcgggca gcacctccac ccgcacgttc agctccggat gctcgaggct
     8461 cacccggaca tcgggccgca gcgcgagcac gtggccgccc aggtcgcggt tgagctccac
     8521 cgacgtcatc ggaaaggtct tgaaggcgcg tcgggcggtg atccggaagg acccgaagac
     8581 gcggcccgcg atcgcctcct ccaccgcggc ccgcagcgcc tcgcgcgaca cgccggtccg
     8641 gatcgcgagg gcgaagttcg ccacgcccgc gacacgcgcc agccgctcgg gcaggagccg
     8701 cggcgcggga tggcgcgaga gatcgagcat gatacgcccg gggagctgga cgagctccac
     8761 cggcccgaga tcctcgatcg cgcgcagcag attctgttgg agctggcgga ggaagagcgg
     8821 ccggttgccc cgcttgaggc tgatctcgtg gtagtggacg atggcggccg gcgcggcgcc
     8881 cgtcatcact cgctccccgc ggggcgccgg tgcttgggcc ggcgccggta caccgtcttc
     8941 gggacctcca ccttcggggg cttggcgggc atcggcttgc ggaccttcgc gtggatcggc
     9001 ttcgggcgct tcaccgggac agtatatccc ggcggtcggg ctcagtagat cgcgcccggg
     9061 aaggtgttga cgcccgcctg ccggccggcc accttgtaca tcttcacccc gcccaccgcg
     9121 ccgccggccc agagcccgtc gccgacgccg tcgtggatgg cccagctccg gccccgcacg
     9181 ctccgggccg cctccggagc gcggaagtcg gaggaggcgt cgagcgccct cacgagcagg
     9241 ccgccgtcgg agaagccggc gtgaatggac tccatcacgt cgcgacccat caggtagccg
     9301 cagccccggc gctcgtagag gatcgcggaa tggtagaaga ggggctcgat gaggtagatc
     9361 tccttgccga tgagggcgca gaagccctcc atcgcgtcga gcacgcggga gagcaggcgc
     9421 aggccgcgcc ggacctggcc gggcgcgagc ccgccccgca tcgcgcggcg ctcctcctcg
     9481 atgttgcggc aggtggtgcc gaagagcgtg tcgcgcccgt ccgggtcccg gtcgatcgcg
     9541 tagcgggcgc cggcggggtc ggtgatctgc acgaaggcca gctccggcac cgagagcggc
     9601 gacatctcca cgtccacgag cagcgcgggg tcgcggtcct cggggctcga gcgcacctcc
     9661 acgcgcgccc acggctggtc cgcgggggcg gtcacgcgca ccagacgcgc gcccgccttg
     9721 tcgcagagcg tgagcggatc gatcgcgaac cgatcgagca gctcctccgg caccagccgc
     9781 gcgtagagcg cctccgcgac ctcgcgcggc agcgcgttga tgtcctggat cgagccgagt
     9841 ccgagcccct cgacgcgcgc gtccatgagg gcggcgccta gcagggcccc gccgccgcgg
     9901 cgccgggctc gagcgcgggg agcagcacgc gggcggcctc gagctcctcc ggggtgttga
     9961 cgttcatgaa gacgacctcg ggggcccggt agcgggcgat ctcgcgcgcc gggatctcga
    10021 gcacgcgcac gtcgtcgtag aagccgatga tcttgaggcg ccccgcgcgc agccgcgtct
    10081 cgatcggcgc caggcaggcc tttgcgtaac aggcgtggag cgtctcccac tgctcgccca
    10141 cgcggggcac gaccacgtcc gcctcccccg cccgctccgt gacgaggcgc gccacctcgg
    10201 gcatcaggaa gggcatgtcg cacgccaccg tgaaggcggc gtcgccggag gccgcctgca
    10261 ggcccgaata gatgccgccc agggagccgt gatcggggaa ggcgtcgggc accatcggca
    10321 cgccgagcga ggcgtagagc tccggcgtgt tggtcacgat cagcaggtcg tcggcgacct
    10381 ggcgcacgac gtgcgcgacc cggtcgatga tgcgccgtcc gccgagctcc agcagggcct
    10441 tcggctggcc gcccatgcgg gtgctccggc cgcccgcctg gatcaccccg gttatccgca
    10501 tggggcctcg acgatcaccg ggccctggcg caggatgcgc ggaggccaga ccgtgcagtc
    10561 cgcgacggtg gagcccgcgc cgccgagtgt cgtcccgccg tccaggatga gctccacgcg
    10621 cccgtcgaag tagcctcgga cgtcctccgc ggtcctgggc ggcggcgccg cgctcggatt
    10681 ggcgctcggc gcggtgaccg gccgcgcggc cgcagcgacg agcgcccgcg ccaccgcgtg
    10741 cccgggcagg cggacgccga cggtgccggt gccgccggtg agcccggcgg gcacgcgcgc
    10801 ggcggcgcgg agcaccagcg tgagcgcgcc cggccagtgc cgcgccatca gggcgcgcgc
    10861 gccgtcgggg atcgcggcgg ccagctccgt caccatgtcg atcgagtcca ccagcacgag
    10921 cagcggcttg tcgtccggac gacccttgac ctcgaagacg cgcgcgatcg cggcgggatc
    10981 cagagcgtcg gcgcccaggc cgtagaagct ctcggtgggg aaggccacga gcccgcccgc
    11041 ctcgaccagc gcgccggcgg cgcgcatcac cgcccgatcg ggctggaccg gatccacgct
    11101 gacgacgcgg gtcgagatcg tcggcgcgct cacccgatcc gtctcagggc gtgatggccg
    11161 atgtcccggc ggtagtgggc gccgtcgaag cgaatgcgct cgacgccagc gtaggccgcg
    11221 cggatcgcgg aggccacgtc gacgcccagg gcctgcacgc ccagcacgcg gccccccgcg
    11281 gtgacgaggg cgtctcccgc catcgcggtg cccgcatgga agacattgat gccgggaagc
    11341 gcggccgccg cggcgacgcc cgtgatgggc cggcccgtct cgtaacggcc cggatagccc
    11401 gcggaggtca tcaccacgca gacggagctc cgcgcgctcc acgagagcgt ggccggcagc
    11461 ccctgcccgg tggcggcggc ctggagcagg gcgagcagat cgccgtcgag gcgcggcatg
    11521 atcgcctggc attccgggtc gccgaagcgg cagttgaact cgatgacctt cggcccctca
    11581 cgcgtgatca tcaggccgac gaagagcacg ccggtgtacg gcgcgccctc cttgcccatc
    11641 gccgcgatca ccggccgcac gatctcggcc atgacgcgat cctgcatggc gtcgctcatg
    11701 accggcgccg gcgagtaggc g
//
LOCUS       DASUFA010000248         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_737444, whole genome shotgun sequence.
ACCESSION   DASUFA010000248 DASUFA010000000
VERSION     DASUFA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737444"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFX87_09250"
     CDS             <1..348
                     /locus_tag="VFX87_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX4995143.1"
                     /translation="DEPSVEKAGRAVLERLDARLLLVTRGSRGMALFEREGPVTFMPI
                     HGSDEIADVTGAGDTVISTFTVGLAARATPGEAAWLANVAGGVVVMKRGTATVTVAEL
                     LAALASNGGRPQR"
     gene            326..814
                     /locus_tag="VFX87_09255"
     CDS             326..814
                     /locus_tag="VFX87_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX4995144.1"
                     /translation="MAAVLSVDEAGRLASGWRAAGKRLVLANGCFDLLHVGHVRYLQA
                     ARALGDALVVGLNSDASVRRLKGPGRPVMPAAERAELIAAVSGVDAVVVFEEDSADAL
                     IARLRPDVHAKGTDYTEQSVPERGTVEAVGAKVAIAGDPKTHSTKDLITTILTRFGQT
                     GR"
     gene            912..1955
                     /gene="waaC"
                     /locus_tag="VFX87_09260"
     CDS             912..1955
                     /gene="waaC"
                     /locus_tag="VFX87_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX4995145.1"
                     /translation="MRPEQLTTKVAIVKLSSLGDVIHAIPVARALRRARPGIHLAWIV
                     EAREYALLKNHPDLDTVVPVDTRLWRRLIRRPSGVREVVGKIGRLRGRIRAARFDVAI
                     DLQGLIKSGLLTAYTGAPLRIGFTADHCREGLNCLFTNRRVRPPVEAVHVVEQYLALL
                     GPLGIPPGPVEFLVPIPARAQLRMDDFLGEQGVKSRDLLVAVNPGAGRANKQWPVAHF
                     RRLVDRLATEPNVRVLVLWGPDEVHMARQIRDGSTARALLAPPTDLHELAALLRRSAL
                     MVANDTGPLHLAAALGTPSLGLFGPTRAERNGPYGPHGRGLQSPDGTMAGLAPEVAVD
                     AALAMLALREAAP"
     gene            1952..2728
                     /locus_tag="VFX87_09265"
     CDS             1952..2728
                     /locus_tag="VFX87_09265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4995146.1"
                     /translation="MSRLSVTIIAWNEEERLRACLESVAWADEIVVVDAESGDKTVPI
                     AHEFTDKVSVRPWPGFAAQKNFAIDQATGDWILSLDADERVTPELRERIRVILKDDGP
                     VDGYAIPRRNIFWGAWVRHGGLYPDYQLRLFRRRAGRFVENAVHESVTVSGRVETLAE
                     PMLHHSYRDLEDFVRRSNRYSTLSAQDWIRRGRRAGLLGLVTRPLGRFFSMYIVHRGF
                     LDGWRGFVLAVLYAEYVFLRMAKVWETRRGRKPGEGDETS"
     gene            2725..>2829
                     /locus_tag="VFX87_09270"
     CDS             2725..>2829
                     /locus_tag="VFX87_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995147.1"
                     /translation="MTGSRERVLSGMRPTGQLHLGNYLGALENWVKLQD"
BASE COUNT          395 a         1013 c         1027 g          394 t
ORIGIN      
        1 gacgagccgt ccgtcgagaa ggcggggcgc gcggtgctcg agcggctcga cgcgcgcctg
       61 ctcctcgtca cccggggcag ccgcggcatg gccctcttcg agcgcgaagg gccggtcacg
      121 ttcatgccga tccacggcag cgacgagatc gcggacgtga caggagcggg cgacacggtg
      181 atcagcacgt tcacggtggg actggccgcg cgcgcgaccc ccggcgaggc cgcatggctc
      241 gccaacgtgg cgggtggcgt cgtggtcatg aagcggggca ccgcgacggt gacggtggcc
      301 gagctgctgg ccgcgctggc gagcaatggc ggccgtcctc agcgttgacg aggccggccg
      361 gctcgcgtcc ggctggcgcg cggcgggcaa gcggctggta ctcgccaacg gctgcttcga
      421 tctcctccac gtggggcacg tccgctacct ccaggccgcc cgcgcgctcg gcgacgcgct
      481 cgtggtcggc ctcaactccg acgcctcggt gcgacggctc aagggcccgg gccgcccggt
      541 gatgcccgcc gcggagcgcg cggagctgat cgccgcggtg agcggggtgg acgcggtggt
      601 cgtcttcgag gaagacagcg ccgacgcgct gatcgcgcgc ctccgtcccg acgtgcacgc
      661 caagggcacg gactacacgg agcagagcgt gcccgagcgc ggcacggtgg aggcggtggg
      721 cgcgaaggtc gccatcgcgg gcgatcccaa gacccactcg accaaggacc tgatcaccac
      781 catcctcacc cgcttcggcc agaccggccg atgaccggaa ctgcgcggag cgtccgcgac
      841 tgggggagtg cgggggccat ttctgggccc ccgcattcaa tagagcctga agacttagac
      901 gagtgtccgc ggtgagaccc gagcaactga ctacgaaagt agccatagta aaactgtcct
      961 ccctcggcga cgtcatccac gcgatcccgg tggcgcgcgc gctccgccgc gcgcggcccg
     1021 gcatccatct cgcatggatc gtggaggcgc gcgagtacgc cctgctgaag aaccatccgg
     1081 acctcgacac ggtggtgccg gtggacacgc ggctctggcg gcggctcatc cggcgcccgt
     1141 cgggagtgcg cgaggtggtg ggcaagatcg gccgcctgcg cgggcgcatc cgcgccgccc
     1201 gcttcgacgt cgccatcgat ctccagggcc tcatcaagag cgggctgctc accgcctaca
     1261 ccggcgcgcc gctgcgcatc ggcttcaccg ccgatcactg ccgcgagggc ctgaactgtc
     1321 tcttcacgaa ccggcgcgtg cgcccgcccg tcgaggcggt gcacgtggtg gagcagtacc
     1381 tggccctgct cggcccgctc ggcatcccgc cggggccggt cgagttcctc gtgccgatcc
     1441 ccgcgcgggc ccagctccgc atggacgact tcctgggcga gcagggcgtg aagagccgag
     1501 acctgctggt ggcggtgaac cccggcgcgg gccgcgcgaa caagcagtgg ccggtcgcgc
     1561 acttccggcg tctcgtggac cggctcgcca ccgagcccaa cgtgagggtc ctggtcctct
     1621 ggggcccgga cgaggtccac atggcgcgcc agatccgcga cggatcgact gcgcgcgccc
     1681 tcctcgctcc gcccacggac ctgcacgagc tggcggcctt gctgcgccgc tccgcgctga
     1741 tggtcgccaa cgacacgggc ccgctgcacc tggccgcagc cctcggcacg ccgagcctcg
     1801 gcctcttcgg gccgacgcgc gcggagcgca acggtcccta cgggccgcac ggccgcgggc
     1861 tccagagccc ggacggcacg atggcgggtc tcgcgccgga ggtggcggtg gacgcggcgc
     1921 tggccatgct cgccctgcgg gaggccgcgc cgtgagccgg ctctcggtga ccatcatcgc
     1981 gtggaacgag gaggagcgcc tgcgcgcctg cctcgagagc gtggcctggg cggacgagat
     2041 cgtggtggtg gacgccgagt cgggcgacaa gacggtaccg atcgcgcacg agttcaccga
     2101 caaggtctcg gtgcgcccgt ggcccggctt cgcggcccag aagaacttcg ccatcgacca
     2161 ggcgaccggc gactggattc tctcgctcga cgccgacgag cgggtgaccc ccgagctgcg
     2221 cgagcgcatc cgcgtgatcc tcaaggacga cggccccgtg gacggctacg cgatcccgcg
     2281 gcggaacatc ttctgggggg cgtgggtgcg ccacggcggc ctctatccgg actaccagct
     2341 ccgcctcttc cggcggcgcg ccgggcgctt cgtggagaac gcggtccacg agtcggtcac
     2401 cgtgtcgggc agggtggaga cgctcgccga gccgatgctg caccactcct accgcgacct
     2461 cgaggacttc gtccgccgct ccaatcgcta ctcgacgctg tccgcgcagg actggatccg
     2521 gcggggccgc cgggccgggc tcctgggcct cgtgactcgg cccctcggcc gcttcttttc
     2581 catgtacatt gttcaccgag gcttcctgga cggctggcgg ggcttcgtgc tcgccgtcct
     2641 ctacgccgag tacgtcttcc tgcgcatggc caaggtgtgg gagacccggc ggggccggaa
     2701 gcccggagag ggtgacgaga cgtcatgacc ggatcgcggg aacgcgtgct gtcggggatg
     2761 cggccgaccg gccagctcca cctcggcaac tacctggggg cgctcgagaa ctgggtcaag
     2821 ctccaggac
//
LOCUS       DASUFA010000249         4185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1096653, whole genome shotgun sequence.
ACCESSION   DASUFA010000249 DASUFA010000000
VERSION     DASUFA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096653"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFX87_09275"
     CDS             complement(<1..843)
                     /locus_tag="VFX87_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4995148.1"
                     /translation="MPPLLRPREPATITLAPRAATVAAECDVLVVGGGPAGLGAAIGA
                     ADAGADAILVERHAFLGGNATAALVMPLMSFHTQRGPAAPGAASELMPHDHGPGDPVV
                     EGVLKRLLDRLEAAGGSLRPSPQTGYVVPFDPEIFKLVALDLLDEAGVRVLLYAFASG
                     VVGAPRPDGVVFETKSGPIVVMARAIVDATGDGDVAALAGAPFEVGRAGDALVQPMTL
                     MFRMVEFERTAFAAYRQAHPDQWRGVHGLWELVREASAAGELALAREDILFFGTPHER
                     ELSVN"
     gene            complement(847..2010)
                     /locus_tag="VFX87_09280"
     CDS             complement(847..2010)
                     /locus_tag="VFX87_09280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX4995149.1"
                     /translation="MTNQAADRVYRIGISGSYGGLNLGDEAILQSIVAQLRAAVPCEI
                     TVFSRDAKDTAARHKVEKSIPVRDLSRDEVRPEVAGLDLLILGGGGILYDDAAAVYLR
                     EVELAQEHSVPVLVYAVSAGPLHDAAARTLVRDTLSRVDAVTVRERRARQLLEEVGVR
                     DIEVTADPAFLLEPEPLPAEALQREGLNQNRRLIGLSVREPGSAAPDIDETHYHALLA
                     NAADYMVDRLDADLVFVPMEPRQKDTQHCHAVVSKMAHAHQATVLKGEYTSGQLLSLM
                     SHFDFAVGMRLHFLIFAALNRVPFVALPYSSKVAGFLEDLEIAMPPLKQVNAGQLIAY
                     IDRWWDLRRNLQARLEKTVPEIQIRARRTTEIALRLLTERRDLRAAEAGRGSA"
     gene            2219..3784
                     /locus_tag="VFX87_09285"
     CDS             2219..3784
                     /locus_tag="VFX87_09285"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX4995150.1"
                     /translation="MTRRRWIVAAVALIALAGLASAIALFFIDEPLRRYTEAKMNARL
                     QGYTATIGALHFSPINFSLELRDTVIVQHEHPDPPVANIGRLYASVHWRALLAGRVVG
                     DILIDRPMVVLDLPQAKEEIDDATPVKDKGWQDALQAIYPLKINELRISRGDVTYKDN
                     GSFKPLRVQDLELEASNIRNMHSEVGVYASPMRLTARVSEGGSVSATGWADFLAEPHA
                     AFMTDIKLDGIELDYFKPITNRYNVVMDKGTLSAYGEVEIAPQYKSVKLWSATVDGVH
                     VDYVHTPTMAGVGKEVAREAARAADKSQDPGLMFRVDRLDVVKSDFGFVNKTVSPSYR
                     VFVKDTTMTLTNLSSLPSGEVSQAKLTGKFMGSGPAGADFKLRPARPGPDFDLAVEIE
                     ETDLKTMNDLLRAHGNFDVTAGRFSMYTEISVRDRRMSGYVKPLFQDMTVYDARQDRE
                     KNVLRKVYEGVVGGISRVLQNRPRDEVATRIEISGPLDRPNAGVIETIAGLVQNAFFK
                     AILPGFEARLHRR"
     gene            3807..4097
                     /locus_tag="VFX87_09290"
     CDS             3807..4097
                     /locus_tag="VFX87_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995151.1"
                     /translation="MAEFKVVCPCCDGRLTIDPALEAVIAHEPAPRAKSGLGLDDALA
                     SLKGAEARRAERFKEELKAQEKKPQVLDKMFKEGIKKAKDMPGRPLNPLDLD"
BASE COUNT          656 a         1506 c         1432 g          591 t
ORIGIN      
        1 gttgaccgac agctcccgct cgtgcggcgt gccgaagaag aggatgtcct cgcgcgcgag
       61 cgccagctcg cccgccgcgc tcgcctcccg caccagctcc cagagcccgt gcacgccgcg
      121 ccactggtcg gggtgcgcct gccggtaggc cgcgaaggcg gtccgctcga actccaccat
      181 gcggaacatc agggtcatgg gctgcacgag ggcgtcgccc gcgcggccga cctcgaaggg
      241 cgcccccgcc agggcggcca cgtccccgtc gccggtggcg tccacgatgg cccgggccat
      301 gacgacgatc ggccccgatt tggtctcgaa caccacgccg tcgggccgcg gcgcgcccac
      361 gacgccgctg gcgaaggcgt agagcaggac gcggacgccc gcctcgtcga ggagatcgag
      421 ggcgacgagc ttgaagatct ccgggtcgaa cggcaccaca tagccggtct ggggcgacgg
      481 ccggagcgag ccgcccgcgg cctccagccg gtccagcagg cgcttcagca ccccctcgac
      541 caccggatcg cccggtccgt gatcgtgggg catcagctcc gaggcggccc cgggcgccgc
      601 gggaccacgc tgggtgtgga aggacatgag cggcatcacg agcgcggcgg tcgcgttgcc
      661 gcccaggaag gcgtggcgct ccaccaggat cgcgtccgcc ccggcgtcgg ccgcgccgat
      721 ggcggccccg aggccggcgg gaccgccgcc caccaccagg acgtcgcact ccgcggccac
      781 ggtggccgcg cgcggcgcca gggtaatggt cgccggctcg cgcggccgga gcagcggggg
      841 catggctcag gccgatcccc ggcccgcctc ggcggcccgg agatcccgcc gctcggtcag
      901 gagccgcagc gcgatctcgg tggtgcgccg cgcccgtatc tggatctccg gcaccgtctt
      961 ctcgagccgc gcctggaggt tgcgccggag gtcccaccac cggtcgatgt aggcgatgag
     1021 ctggcccgcg ttgacctgct tgagcggcgg catcgcgatc tcgagatcct cgaggaagcc
     1081 cgcgaccttg ctcgagtagg gcagcgccac gaacggcacc cggttgagcg ccgcgaagat
     1141 caggaagtgc aggcgcatgc ccaccgcgaa gtcgaaatgg ctcatgagcg acaggagctg
     1201 gcccgacgtg tactcgccct tgagcacggt ggcctgatgc gcgtgggcca tcttggacac
     1261 caccgcgtgg cagtgctggg tgtccttctg acgcggctcc atgggcacga acaccagatc
     1321 ggcgtcgagc cggtccacca tgtagtcggc cgcgttggcg agcagcgcgt ggtagtgcgt
     1381 ctcgtcgatg tcgggcgcgg ccgagcccgg ctcccggacc gacaggccga tcaagcgccg
     1441 gttctggttc aggccctcgc gttgcagcgc ctccgcgggc agcggctcgg gctcgagcag
     1501 gaacgcggga tcggcggtca cctcgatgtc gcgcacgccc acttcctcga ggagctgccg
     1561 ggcgcgccgc tcccggaccg tcaccgcgtc cacccggctc agggtatcgc gcaccagggt
     1621 ccgcgccgcc gcgtcgtgga gcgggcccgc gctcaccgcg tagaccagga ccggcacgct
     1681 gtgctcctgc gcgagctcga cttctctcag atagacggcg gccgcgtcgt cgtagaggat
     1741 gccgccgccg cccaggatca acagatcgag cccggcgacc tccggccgca cctcgtcgcg
     1801 cgagagatcg cgtaccggga tgctcttctc caccttgtgg cgcgccgcgg tgtccttggc
     1861 gtcgcgcgag aacaccgtga tctcgcaggg cacggcggcg cggagctgcg ccacgatgga
     1921 ctggaggatg gcctcgtcgc ccaggttgag cccgccgtac gagcccgaga tgccgatccg
     1981 gtacacgcgg tccgcggcct gattggtcat ggacccgatc gcttcaactc ctgtgccacg
     2041 gagaaccggc gcgatccacc gcgttttgcc gcggaaggaa ccgccggtcg gtcggaactt
     2101 cccggtcgcc cccggtagga acttcccggg ccggttccgg cgccgccgcc cgcgccccgc
     2161 gcggccgccg gggatgtcgt gggcatgcgc cttgctaggt cggagtcgca tgatcaccat
     2221 gacccgccgc cggtggatcg tcgcggccgt cgccctgatc gccctggccg gcctcgcctc
     2281 cgccatcgcg ctcttcttca tcgacgagcc gctgcgccgg tacaccgagg cgaagatgaa
     2341 cgcgcgcctg cagggctata ccgccacgat cggcgcgctg catttcagcc ccatcaactt
     2401 ctcgctcgag ctgcgggaca ccgtgatcgt ccagcacgag catcctgatc cgccggtggc
     2461 gaacatcggg cggctctacg cgagcgtgca ttggcgggcg ctcctggccg gccgcgtggt
     2521 cggcgacatc ctcatcgacc gcccgatggt ggtcctcgat ctgccccagg cgaaggaaga
     2581 gatcgacgac gcgacccccg tgaaggacaa ggggtggcag gacgcgctgc aggccatcta
     2641 tcccctcaag atcaacgagc tgcggatctc gcggggcgac gtcacctaca aggacaacgg
     2701 gtccttcaag cccctgcgag tccaggatct cgagctcgag gcctccaaca tccgcaacat
     2761 gcactccgag gtcggggtgt acgcctcgcc gatgcgcctc accgcgcgcg tgtccgaggg
     2821 cggctcggtc agcgccaccg gctgggccga tttcctcgcc gagccccacg ccgcgttcat
     2881 gaccgacatc aagctcgacg gcatcgagct cgactacttc aagcccatca ccaaccgcta
     2941 caacgtggtg atggacaagg gaaccctgtc cgcgtacggg gaggtcgaga tcgcgcccca
     3001 gtacaagtcg gtgaagctgt ggagcgccac cgtggacggc gtgcacgtgg actacgtgca
     3061 caccccgacg atggccgggg tgggcaagga agtggcgcgc gaggcggcga gggccgccga
     3121 caagtcccag gacccgggcc tgatgttccg ggtggaccgg ctcgacgtgg tcaagagcga
     3181 tttcggcttc gtgaacaaga cggtgagccc gagctatcgg gtcttcgtca aggacaccac
     3241 gatgaccctc accaacctga gcagcctgcc cagcggagaa gtctcgcagg ccaagctgac
     3301 cggcaagttc atgggcagcg gaccggccgg cgccgacttc aagctgcgcc cggcccggcc
     3361 gggccccgac ttcgacctgg ccgtcgagat cgaggagacc gacctcaaga cgatgaacga
     3421 tctgctgcgg gcccacggca acttcgacgt gacggccggt cgcttctcga tgtacaccga
     3481 gatctcggtc agggaccggc ggatgtccgg ctacgtcaag ccgctcttcc aggacatgac
     3541 cgtctacgac gcgcgccagg atcgagagaa gaacgtgctg cggaaggtct acgagggcgt
     3601 ggtgggcggc atctcgcgcg tgctgcagaa ccggccgcgg gacgaggtcg cgacccgcat
     3661 cgagatctcc ggcccgctcg acaggcccaa cgccggcgtc atcgagacca tcgccggcct
     3721 ggtccagaac gcgttcttca aggcgatcct gcccggcttc gaggcccgcc tgcaccgccg
     3781 ctgagcctct gtggtagtct cgggccatgg cggagttcaa ggtcgtctgc ccctgctgcg
     3841 acgggcgcct caccatcgat ccggcgctgg aggcggtcat cgcgcacgag ccggcgcccc
     3901 gggcgaagtc ggggctcggc ctcgacgacg cgctcgcctc cctcaagggc gccgaggcgc
     3961 gccgggccga gcgcttcaag gaagagctca aggcgcagga aaagaagccg caggtcctcg
     4021 acaagatgtt caaggagggg atcaagaagg ccaaggacat gcccggccgc cccctcaatc
     4081 cgctcgacct cgactagccg ccgtgcccgg aagcgatctc accgccgctc tcctccggcg
     4141 ggtcagtcga tccttctatc tctcgctcac cgtcctgccc gggtc
//
LOCUS       DASUFA010000250         7992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_632567, whole genome shotgun sequence.
ACCESSION   DASUFA010000250 DASUFA010000000
VERSION     DASUFA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632567"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFX87_09295"
     CDS             complement(<1..261)
                     /locus_tag="VFX87_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX4995152.1"
                     /translation="MQSLTVMAPHSLGSGTISFGLVSIPVKLYAAASSGGVSFNLLHA
                     KCGSRLKQQYICPKDNEIVDRDSMIKGYEFAKGQYVTFTSEEL"
     gene            296..3064
                     /gene="ligD"
                     /locus_tag="VFX87_09300"
     CDS             296..3064
                     /gene="ligD"
                     /locus_tag="VFX87_09300"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX4995153.1"
                     /translation="MATARDGGARREKGRRIGPELTTYRKKRDPERTPEPFGGRPPTG
                     GRLFVVQKHAARRLHYDLRLEMDGVLKSWAVPKGPSVHAEEKRLAVHVEDHPIEYGDF
                     EGVIPPGNYGAGAVIVWDRGWYRSAKPEDPLTQLARGKLEVEIFGHKMRGRWTLARMS
                     GKDKDWLLLKKADGAAVPPDAAELTERYPQSVLSGLTVEEMANASGRLATIRARLDAL
                     GAPRGEVVARRQPFTLATLVEDPFSDPAWLFEIKYDGVRVLASRRGDRVELYGRSGQD
                     TTSRYPEVVRALRALPVESFVIDGEIVALDDAGRPSFQRLQPRMALTDPREIERMAAQ
                     RPAIGVFFDCLMLDDRDLRKLPLVDRKECLRLLVPSLGAVRYGDHVMEEGKAFFELAS
                     EQRLEGMVAKRARSVYSGARTRDWIKIKCQRRQEFVIGGYTEPQGSRGHFGALHLGVY
                     DGPAGAPRLVYVSKVGTGFDQAGLKAIWEKLRPIARATPPFEAGAIPTGRAHHWVEPR
                     LVAEVRFTDWTEDGGLRHPTFIGLRDDKKPLECRREEVLEEPPLTLPSPQRGEGGEES
                     ASPSPPGGGEGRVRGREEAGPRLKPTNVKKVFWPAEGYTKGDLIAYYERVAPLMLPYL
                     RDRPLVLTRFPDGITGKSFYQKDAPDFAPAWVRTERIYSRDTERDIAYLVVDDVEMLR
                     YVANSGAIPIHLWASRVPSLERPDWLVLDLDPKGAPFTDVVRVALALRRILERLELPS
                     YVKTSGATGLHILLPLGARYTYEQCRTFARLLAVMGVEAEPEISTVDRPLRARGGKVY
                     IDFGQNGHGQTIVAPYSLRPLPGAPASCPLEWREVTPRLDPARFTLRTLPVRFDKMAD
                     PLTPVLGDGIDMAAALERIEALHTNKPSPLREEGRVRGGGAKGSLSGRAAPGAGGRTR
                     GRSRPPRA"
     gene            complement(2997..3698)
                     /locus_tag="VFX87_09305"
     CDS             complement(2997..3698)
                     /locus_tag="VFX87_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX4995154.1"
                     /translation="MIAIGTSGYNYPEWRGTFYPEKFPAAKMLPYYAERFSTVEINYT
                     FYRMPNAKTIAGWVAETPAGFSFTLKAPQRITHFARLREIGDPLRYFLDTAAGLGPKL
                     GPVLFQLPPNFKKDLVRLGGLLDQLPAGLRCAMEFRHETWFSDDVYERLRAVNAALCV
                     ADTEEATTPVEVTADWGYLRLRDQGYTSADLEGWGRRIRELGARWRDTFVYFKHEEAG
                     TGPAFARQLRELLDR"
     gene            complement(3695..4498)
                     /locus_tag="VFX87_09310"
     CDS             complement(3695..4498)
                     /locus_tag="VFX87_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX4995155.1"
                     /translation="MKIEMVEDPTDPRIAGFRDARDGELRRREGLFLAEGRLLVRRLL
                     ETSRFPVQSLLLTPPALDDLRDLLADDDSMPVHVASAETIRAIVGFKFHRGCLALGVR
                     SEPVAPETLMGPAGPRTLLALEDVVDPDNVGAIFRNAAAFGAAGVLLSSHCADPLYRK
                     AIRVSMGATLSLPFAQVPDWGVALATLRHAGYRLVALCPGAEAEDIVEVAGRAPRRPR
                     LALLLGAEGGGLSAATRAAADVQVRIPMAAGVDSLNVAAACGIALHRFA"
     gene            complement(4589..4990)
                     /locus_tag="VFX87_09315"
     CDS             complement(4589..4990)
                     /locus_tag="VFX87_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995156.1"
                     /translation="MRNRDRVHGPRESAARQHVTDPHPPEVRRKRGRPKGSTGISRRH
                     PDSVLAAEIAKTPLATVYASHDGELYHAICKQPLEFQGRRAQLELDFYCLRCMEHVAL
                     PQFALSRIRFDLPRPADVDLPEWATSIVAYQ"
     gene            5181..5468
                     /locus_tag="VFX87_09320"
     CDS             5181..5468
                     /locus_tag="VFX87_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018147903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX4995157.1"
                     /translation="MLGVVATIKVKSGMESQFEAVAKELVAKVTANEPGCRLYVLHRG
                     EAPQTYVFMERYADQAAVEAHRATDYFKALGRKMGEFMEGRPEVLRLKEVE"
     gene            complement(5473..5736)
                     /locus_tag="VFX87_09325"
     CDS             complement(5473..5736)
                     /locus_tag="VFX87_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="involved in start site selection during the
                     initiation of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEX4995158.1"
                     /translation="MAAPPVPRDGVVRLFRDRGGRNGKTVTVIHGLPERGPALEARLS
                     EMKRLCGAGGTLKDGVLEIQGDHRERLAARLRALGYTVKLAGG"
     gene            5768..6877
                     /locus_tag="VFX87_09330"
     CDS             5768..6877
                     /locus_tag="VFX87_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX4995159.1"
                     /translation="MKITDVTLTLFAWENIPATIYGRHTGKFGGQSQLGLLTLRTDEG
                     AEGHAFLGSASRSAQYDAESLISALKPLVMDQDPLDRERLYQALLSRSRQTTWRAIGA
                     VDVALWDLAGKVTGLPIHRLLGSYRERLPAYASSAVLPSKQAYAEEAARFKGSGWTAY
                     KIHPPTDPRLDIEICEAVRGMVGDAFTLMLDSTWAYDYPAAIRVGRAVERLGYYWYED
                     PLADDDLYNYVKLKQHLSIPILATEYAPGGFTAYAPWLVARATDYLRGDVAVKGGISA
                     LVKTAHLAEGFHMNFEIHHGGNSLNNVANLHVAMAIRNCEFFEVLLPAEAQKYGLVAD
                     IEVDSHGMVRAPTGPGLGAQIDFALIERKKVATLG"
     gene            complement(6890..7780)
                     /locus_tag="VFX87_09335"
     CDS             complement(6890..7780)
                     /locus_tag="VFX87_09335"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX4995160.1"
                     /translation="MLLRSLGRLLNRRPSGPIAPPAPLETVLVGDDSPAALTRLLCGR
                     DNPAREAALPRYLQYRGIAFSRHRFTRPEGRGDTFAVELGEGPRVLVLCAHHDAVPGS
                     PGANDNAAAVGILLHLIPRLPARIPRGWRVRLLFTAAEELDYLGARAYVEDTPLSDVA
                     GVLSLELCGIGDTVALWDAGEPTPFLGGVTAALESIGLAREAGYHVIGRIPVFGSDHR
                     AFAAAGIPAYGFTMVPAAHADALRRFVLSPVRSSVRSLIRRPPPFDTYHTSDDALETL
                     EPAALHRAAAALTAVVAELD"
     gene            complement(7807..>7992)
                     /locus_tag="VFX87_09340"
     CDS             complement(7807..>7992)
                     /locus_tag="VFX87_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX4995161.1"
                     /translation="KGTMYGSVRPNIDFPKLVDLYLDGRLKIDELVSRTYKLDEINEG
                     FTALRTGQVARGVVVFN"
BASE COUNT         1240 a         2734 c         2897 g         1121 t
ORIGIN      
        1 gagctcctcg ctggtgaagg tcacgtactg gcctttggcg aactcgtagc ccttgatcat
       61 cgaatcgcgg tcgacgatct cgttgtcctt gggacagatg tattgctgct tcaggcgaga
      121 gccgcatttg gcgtgcagca ggttgaagct caccccgccc gacgacgcgg cggcgtacag
      181 cttcaccggg atcgagacga gcccgaagga aatggtgccg gatccgagag aatgaggcgc
      241 catgaccgtg aggctttgca cactaccacg caggcagcta tgctccaaga ctccgatggc
      301 aaccgcacgg gatggtggtg cccgccgcga aaaggggcga cgtattgggc ccgagctcac
      361 cacgtaccgg aagaagcgcg acccggagcg cacgccggag ccgttcgggg ggcggccgcc
      421 cacggggggc cggctcttcg tggtgcagaa gcacgcggcg cggcggctgc actacgacct
      481 gcgcctcgag atggacgggg tgctgaagag ctgggcggtg ccgaaggggc cctcggtgca
      541 cgcggaggag aagcggctcg cggtccacgt cgaggatcac cctatcgagt acggcgactt
      601 cgagggcgtg atcccgcccg gcaactacgg cgccggcgcg gtcatcgtgt gggaccgcgg
      661 ctggtaccgg tccgccaagc ccgaggatcc gctcacgcag ctcgcgcgcg gcaagctcga
      721 ggtggagatc ttcggccaca agatgcgggg gcgctggacg ctggcccgca tgtccggcaa
      781 ggacaaggac tggctgctgc tcaagaaggc cgacggcgcc gcggttccgc cggacgcggc
      841 cgagctgacc gagcgctatc cgcagtcggt gctgtccggg ctgaccgtcg aggagatggc
      901 gaacgcctcc gggcggctcg ccacgatccg cgcgcgcctc gacgcgctcg gggcgccccg
      961 cggcgaggtg gtcgcgcggc ggcagccctt cacgctggcc acgctcgtcg aggacccgtt
     1021 ctcggatccg gcctggctct tcgagatcaa gtacgacggc gtgcgcgtgc tcgcctcccg
     1081 tcgcggcgac cgcgtcgagc tgtacggccg gagcggccag gacaccacct cgcgctatcc
     1141 ggaggtggtg cgcgccctgc gcgcgctgcc ggtggagtcc ttcgtcatcg acggggagat
     1201 cgtggccctc gacgacgcgg ggcggcccag cttccagcgg ctgcagccgc gcatggcgct
     1261 caccgatccg cgcgagatcg agcggatggc cgcgcagcgt cccgcgatcg gcgtgttctt
     1321 cgactgcctg atgctcgacg accgcgatct gcggaagctg cccctcgtgg accgcaagga
     1381 gtgcctgcgg ctcctggtgc cgtcgctcgg cgccgtccgc tacggcgatc acgtcatgga
     1441 ggaggggaag gccttcttcg agctggcctc cgagcagcgc ctcgagggca tggtggccaa
     1501 gcgggcgcgg agcgtctatt cgggcgcgcg cacgcgcgac tggatcaaga tcaagtgcca
     1561 gcgccgccag gagttcgtga tcggcggcta caccgagcct caaggctcgc gcgggcactt
     1621 cggcgcgctg catctgggcg tctacgacgg ccccgcggga gcgccccgcc tcgtctacgt
     1681 ctcgaaggta ggcaccggct tcgatcaagc cggactcaag gcgatctggg agaagctccg
     1741 cccgatcgcc cgcgcgacgc cgccgttcga ggccggcgcg atcccgacgg gccgcgccca
     1801 ccactgggtg gagccgcgtc tcgtcgccga ggtgcgcttc accgactgga ccgaggacgg
     1861 cggcctccgc cacccgacgt tcatcggcct gcgcgacgac aagaagccgc tcgagtgccg
     1921 ccgcgaagaa gtcctggagg agccgcccct caccctgccc tctccccaga ggggagaggg
     1981 gggagaagag agcgcgtctc cctctccccc gggaggggga gagggcaggg tgagggggcg
     2041 cgaggaagca gggccccggt tgaagcccac caacgtcaag aaagtcttct ggccggccga
     2101 aggctacacc aagggcgacc tgatcgcgta ctacgagcgg gtggcgccgc tgatgctgcc
     2161 gtatctccgg gatcggccgc tcgtgctcac gcggtttccg gacggcatca ccggcaagtc
     2221 cttctaccag aaggacgcgc cggacttcgc gccggcctgg gtgcgcaccg agcgcatcta
     2281 ctcgcgggat accgagcgcg acatcgcgta cctggtcgtg gacgacgtcg agatgctgcg
     2341 ctatgtggcc aatagcggcg cgatcccgat tcatctctgg gcctcgcggg tcccgtcgct
     2401 cgagcgcccg gactggctgg tgctcgacct cgaccccaag ggcgcgccgt tcaccgacgt
     2461 ggtgcgggtc gcgctcgccc tgcgccgcat cctggagcgg ctcgagctgc cgagctacgt
     2521 gaagacctcg ggcgccaccg ggctgcacat cctgctgccg ctgggcgcgc gctacaccta
     2581 cgagcagtgc cggaccttcg cgcggctgct cgccgtgatg ggtgtggagg ccgagcccga
     2641 gatctcgacg gtggaccgtc cgctccgtgc ccggggaggc aaggtctaca tcgacttcgg
     2701 ccagaacggc cacggccaga ccatcgtcgc cccctactcg ctgcgcccgc tgcccggcgc
     2761 ccccgcctcc tgcccgctcg agtggcgcga ggtgacgccg cgcctcgatc cggcgcgctt
     2821 cacgctcagg actctgcccg tgcgcttcga caagatggcg gatccgctga cgccggtgct
     2881 cggcgacggc atcgacatgg ccgccgccct cgagcgcatc gaagccctcc acaccaacaa
     2941 accttctccc ctcagggaag agggcagggt gaggggtggt ggcgcaaagg gcagcctcag
     3001 cggtcgagca gctcccggag ctggcgggcg aacgcggggc cggtcccggc ctcctcgtgc
     3061 ttgaagtaga cgaaggtatc gcgccaccgc gcgccgagct cccggatccg ccggccccac
     3121 ccctcgagat cggcggaggt gtagccctgg tcgcgcaggc gcaggtaacc ccagtccgcg
     3181 gtcacctcga cgggtgtggt ggcctcctcg gtatcggcca cgcagagcgc ggcgttgacc
     3241 gcgcgcaggc gctcgtacac gtcgtccgag aaccatgtct cgtggcggaa ctccatcgcg
     3301 cagcgcaggc ccgcggggag ctggtcgagc aggccgccca gccggaccag gtccttcttg
     3361 aagttgggcg ggagctggaa cagcaccggg ccgagcttcg gcccgaggcc ggccgcggta
     3421 tcgaggaaat agcgcagcgg atcgccgatc tcgcgcagcc gcgcgaagtg ggtgatgcgc
     3481 tgcggcgcct tcagcgtgaa cgagaagccc gcgggcgtct ccgcgaccca gccggcgatg
     3541 gtcttggcgt tgggcatccg gtagaaggtg tagttgatct ccaccgtcga gaagcgctcg
     3601 gcatagtagg gcagcatctt ggcggcgggg aatttctccg ggtagaaggt gccccgccac
     3661 tccggatagt tgtagccgga cgtgccgatg gcgatcatgc gaagcgatgg agcgcgatcc
     3721 cgcaggccgc cgcgacgttg agcgaatcca ccccggcggc catcgggatg cgtacctgga
     3781 cgtccgcggc ggcccgcgtc gccgcgctga gccccccgcc ctcggcgccc agcaggagcg
     3841 cgagcctcgg ccggcgcggc gcccggcccg ccacctcgac gatgtcctcc gcttccgccc
     3901 cgggacagag cgccaccagc cggtacccgg cgtggcgaag ggtcgcgaga gctacccccc
     3961 agtccggcac ctgcgcgaac ggcagcgaga gggtggcgcc catggagacg cggatcgcct
     4021 tgcggtagag cggatcggcg cagtggctcg agaggagcac gccggccgcc ccgaaggccg
     4081 ccgcgttccg gaagatcgcg ccgacattgt cgggatccac cacgtcctcg agcgccagca
     4141 gcgtgcgcgg gccggccggg cccatgagcg tctcgggcgc gacgggctcg ctgcgcacgc
     4201 ccaaggccag gcagccgcgg tggaacttga acccgacgat ggcgcggatc gtctcggcgg
     4261 acgcgacatg aacgggcatc gagtcgtcgt cggcgagcag gtcgcggaga tcgtcgaggg
     4321 ccggtggtgt cagcagaagc gactggacgg gaaagcgaga ggtctcgagc aggcggcgga
     4381 ccaggagacg gccctcggcc aggaacaggc cttcgcggcg ccgcaattca ccgtcacgcg
     4441 cgtcgcgaaa tccggcgatg cgcgggtcgg tgggatcctc gaccatttcg atcttcaaag
     4501 ccgcctccgt ggcggctatg atcccatggc gaggaaggga cgggaaagag cccggctgga
     4561 ccgggccctt cgagcgaggc gagcgagctt actggtaggc gacgatgctc gtagcccact
     4621 ccggaaggtc gacgtcggcc ggccggggga gatcgaagcg aatgcggctc agcgcgaact
     4681 gagggagcgc gacgtgctcc atgcagcgca ggcagtagaa atcgagctcg agctgggcgc
     4741 ggcggccctg gaactcgagc ggctgcttgc agatcgcgtg gtagagctcg ccgtcatggc
     4801 tcgcgtagac ggtcgcgagc ggcgtcttgg cgatctcggc ggccaggacc gagtccggat
     4861 ggcgccggct gatgccggtg ctacccttcg gacggccgcg ctttcggcgg acctccgggg
     4921 ggtggggatc ggtgacgtgc tgcctggctg cgctctcccg ggggccgtgt acgcgatctc
     4981 gatttctcat gcaaggcttc ggagcaagac gcgttccaca ggtgacactg cttctaacct
     5041 actggaattc ctgacatcgc ccccgggtaa cgggggccat gggggcccgg gaaccaacaa
     5101 aattcggcgc accatggtcg gaggttgtca tctcatcaac agcgggtgtc cgatccgggc
     5161 acgccgcctg gaggccagcg atgctcggtg tcgtcgcgac gatcaaggtg aagtcgggga
     5221 tggagtcgca gttcgaggcc gtggccaagg agctggtggc caaggtcacc gccaacgagc
     5281 cgggctgccg gctctacgtg ctgcatcgcg gcgaggcgcc ccagacctac gtgttcatgg
     5341 agcgctacgc ggaccaggcc gcggtggagg cccaccgcgc gaccgactac ttcaaagcgc
     5401 tgggccggaa gatgggcgag ttcatggagg gccgtccgga agtcctccgc ctgaaggaag
     5461 tcgagtagtg gctcagccgc cggccagctt cacggtatag ccgagcgcgc gcagccgtgc
     5521 ggcgaggcgc tcccggtggt cgccctggat ctcgagcacg ccgtccttca gggtgccgcc
     5581 ggcgccgcag agccgcttca tctcggagag gcgggcctcc agcgcggggc cgcgctcggg
     5641 cagcccgtga atcacggtca cggtcttgcc gttgcgtccg ccgcgatcgc ggaagagccg
     5701 gacgacgccg tcgcgcggaa cgggtggagc agccaccgcc agaatctagc agaaggggag
     5761 gagcctcgtg aagatcaccg acgtcacgct cacgctgttc gcctgggaga acattcccgc
     5821 caccatctat ggccggcaca ccggcaagtt cggtggccag agccagcttg ggctgctcac
     5881 cctccgcacc gacgagggcg cggaaggcca cgccttcctg ggctcggcga gccgcagcgc
     5941 ccagtacgac gccgagtcgc tgatcagcgc gctcaagccc ctcgtgatgg accaggatcc
     6001 cctggaccgg gagcggctct accaggccct cctctcgcgc agccgccaga ccacgtggcg
     6061 cgcgatcggg gcggtggacg tggcgctgtg ggacctcgcg ggcaaggtga ccggtctccc
     6121 gatccaccgg ctgctcggct cctaccggga gcgcctgccc gcctacgcga gctccgcggt
     6181 gctgccgtcc aagcaggcct acgcggagga ggcggcgcgc ttcaagggct ccggctggac
     6241 cgcctacaag atccacccgc cgaccgaccc ccggctcgac atcgagatct gcgaggcggt
     6301 gcgcgggatg gtgggcgacg ccttcaccct catgctcgac tccacctggg cctacgacta
     6361 cccggccgcg atccgggtgg gacgggccgt cgagcggctc ggctactact ggtacgagga
     6421 cccgctggcc gacgacgacc tctacaacta cgtcaagctc aagcagcacc tctcgatccc
     6481 catcctcgcc accgagtacg cgccgggcgg cttcaccgcc tacgcgccct ggctcgtcgc
     6541 ccgcgcgacc gactatcttc gcggagacgt ggcggtaaag gggggcatct cggcgctcgt
     6601 gaagaccgcg cacctcgccg agggcttcca catgaacttc gagatccacc acgggggaaa
     6661 ctcgctcaac aacgtggcca acctccacgt ggcgatggcg atccgcaact gcgagttctt
     6721 cgaggtgctg ctgcccgcgg aggcccagaa gtacgggctc gtcgccgaca tcgaggtgga
     6781 ctcgcacggc atggtgcgcg cgcccaccgg ccccgggctg ggggcccaga tcgacttcgc
     6841 gctgatcgag cgaaagaagg tcgcgacgct cggctgaccg ccgggtgccc tagtcgagct
     6901 ccgcgaccac cgcggtgagc gcggccgccg cgcggtggag cgccgcgggc tcgagcgtct
     6961 cgagcgcgtc gtcgctcgtg tggtaggtgt cgaacggggg cgggcgacgg atcaggctcc
     7021 gcacgctcga gcgcaccggg ctcagcacga agcgacgcag cgcgtccgcg tgggccgccg
     7081 gcaccatggt gaagccgtag gcggggatgc ccgcggccgc gaaggcccgg tggtcgctac
     7141 cgaagaccgg gatgcgcccg atcacgtgat agcccgcttc gcgggccagc ccgatggact
     7201 cgagcgccgc cgtcaccccg cccaggaacg gggtgggctc gcccgcgtcc cagagggcca
     7261 ccgtgtcgcc gatcccgcac agctcgaggc tcagcacgcc ggccacgtcg ctgagcggcg
     7321 tgtcttccac gtaggcgcgc gcgcccaggt agtcgagctc ctcggcggcg gtgaagagca
     7381 ggcggacccg ccagccgcgc gggatccggg cgggcaggcg tgggatcagg tgcagcagga
     7441 tcccgacggc ggccgcgttg tcgttggcgc cggggctgcc ggggaccgcg tcgtgatgcg
     7501 cgcacagcac gaggacgcgc gggccctcgc cgagctcgac cgcgaacgtg tccccccggc
     7561 cctccgggcg ggtgaagcga tggcgcgaga acgcgatgcc gcggtactgg agatagcggg
     7621 gcagggccgc ctcccgcgcc gggttgtcgc ggccgcagag gaggcgggtg agcgcggcgg
     7681 gcgagtcgtc gcccaccagc acggtctcga gcggcgcggg cggcgcgatg gggccgctgg
     7741 ggcggcggtt gagcagccgt cccagcgacc gcagcagcaa tcgggccgcc gcgtgggcga
     7801 ccgttctcag ttgaagacga ccacgccgcg ggcgacctgg ccggtccgca gcgcggtgaa
     7861 gccctcgttg atctcgtcaa gcttgtaggt gcggctgacg agctcgtcga tcttgaggcg
     7921 tccgtcgagg tagaggtcca ccagcttcgg gaagtcgatg ttcgggcgta ccgagccgta
     7981 catcgtgccc tt
//
LOCUS       DASUFA010000251         5655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1434336, whole genome shotgun sequence.
ACCESSION   DASUFA010000251 DASUFA010000000
VERSION     DASUFA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434336"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFX87_09345"
     CDS             complement(<1..395)
                     /locus_tag="VFX87_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX4995162.1"
                     /translation="MSESEKDLPRDWRGVVEAAEAGARLDAWLAGRLEGLSRMRVKGL
                     IEAGRVVVGGRVVKAAYRLQPGERVEVSIPPAPRDGLAAEAVPLSVVFEDEHVLVVDK
                     PVGMVTHPGAGRPTGTLAAAALAHAPGMAG"
     gene            complement(442..918)
                     /gene="lspA"
                     /locus_tag="VFX87_09350"
     CDS             complement(442..918)
                     /gene="lspA"
                     /locus_tag="VFX87_09350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX4995163.1"
                     /translation="MKSAGLTALVVLVLDLLTKYLALERLPPGRPVHVIDGLFSLTLV
                     MNPGLAFGMLAGTPAGWRWLVALLSIGALAVLAVVGLRMLPTGGRLTPLALGLIFGGA
                     VGNLIDRARFGAVVDFLDFYWRGYHWPAFNVADASISVGVVLLALRMLTAPPSPAR"
     gene            complement(915..3761)
                     /gene="ileS"
                     /locus_tag="VFX87_09355"
     CDS             complement(915..3761)
                     /gene="ileS"
                     /locus_tag="VFX87_09355"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX4995164.1"
                     /translation="MDYKDTLNLPKTAFPMKANLPKMEPEWLERWAAMDIYGRIRQAS
                     ASRPLWILHDGPPYANGHIHMGTALNKILKDLVVKSRSMLGNNAVYVPGWDCHGLPIE
                     HQVDKELGLDEASVDVRQAMDPMEKRRRCREYASKFIDIQREEFRRLGVFGDWNDPYR
                     TMAPAYQAVIAREFGRFVGRDLVHKGLKPVHWCMHCKTALAQAEVEYEDQTTPSVYVK
                     FPVQTVPAEIARAVGGRPVSLVIWTTTPWTLPANLAIAVHPGETYTAVELDGQALIVA
                     QPLLEAFLRLPGVKGRGAALPLAVPGRTLEGMVARHPWIDRDVPVLTADFVAMDAGTG
                     LVHIAPGHGEEDYELGRRAGLKIYNPVDDDGRFIAEVASFAGQTVWEANPRIIEHMRA
                     AGALVAQVPLQHTYPHCWRCKNPTLFRATEQWFIALDRHGLRQKALDAIRNEVTWIPG
                     WGEERIYNMVAHRPEWVISRQRVWGVPIIAFYCASCDGLLLDEALVAHVAGIMRDGAG
                     ADEWYTREAAALLPAGTRCPRCGGGEFRKETDILDVWFDSGCSHAAVLETRPELRWPA
                     EMYLEGSDQHRGWFHSSLLEAVGTRDKPPYRSVLTHGFVVDGEGRKMSKSGGNNISPD
                     ELIPKYGAEVLRLWVAAEDSSEDIRLSHEILNRLADAYRRIRNTSRFLLGTLADFDPE
                     RDRRPYGDMDELDHWALLRLGGLIARVRRAYEDYQFHVAFHAIHNFCAVDLSSLYLDI
                     IKDRLYVSAPDDSRRRASQTVCFEVLTALTRLMAPILTFTSDEVWSHIPGRGKPESVH
                     LVLFPDERGEWLNERLSADWERLLEVRGEVSRALEAARQQGRIGKGIDAVLYIPSAPE
                     EQWLPLLEAKGQALLTTLFNVSGTRLKQRAPGEAVRYESQDIPGLVLEIVPAQTLGWK
                     KCERCWTWSPRVGEDPAHPTLCERCAPVVTALQR"
     gene            complement(3832..4455)
                     /locus_tag="VFX87_09360"
     CDS             complement(3832..4455)
                     /locus_tag="VFX87_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX4995165.1"
                     /translation="MKGLHPVLAMGLALGSLLSGAITPASAQIYRWTDERGEVRFSQG
                     IHSVPPQFRGAAVMVAAPSQPAPTTPEPSAPSAQPAGVARIPFTPGQPIVVSAKINGG
                     GTTQLILDTGAQGTLISPTALAALGISYRNAVRGSIKGVTGDANVLAVRIESIEVEGA
                     RFGPLMVVSHDSGLGSGRDGLLGRDFLDNFIVTIDSVARVVTLTPKK"
     gene            complement(4452..>5655)
                     /locus_tag="VFX87_09365"
     CDS             complement(4452..>5655)
                     /locus_tag="VFX87_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995166.1"
                     /translation="EVALAAMLVTAALLLHARGRTWAAAAVLGLAALARPEAALLLPL
                     FWLAGPLTGRRALAWGLPVAGFLAPWVAFNLATTGTPLPATAAAKIEGGLVGYLAGVR
                     EPLRMTLLQRPWQFEAEWARWLWRVDTLLPLLLVPGLWWLGRHVGRAALAPASVLVLH
                     PLAMAMLAPYRAPSFQEGRYCIHLLPLALVVAVVPLTPLFSSALSPPGWPRWLRGRLG
                     WLASAALLVGAVVALPSAASRYGWAVQNIETMQVHLGHWVARHTPPTARIGLNDVGAI
                     AYISRREVVDVMGLVTPAIIPFRRDGEMGVLRYLEQACPDYLIVFPAWFPMISEMADR
                     FHPVYRVRLDHNEVAGADELVAYETAWSRWRPDRRPCPGALARGLGTPRETPAEIPGN
                     RIIATRYP"
BASE COUNT          869 a         2006 c         2035 g          745 t
ORIGIN      
        1 ccggccatcc cgggtgcgtg cgcgagggcg gccgcggcca gggtgccggt cggccgcccg
       61 gctcctggat gggtcaccat ccccaccggc ttgtccacga cgagcacatg ctcatcctcg
      121 aacacgacgc tcagcgggac cgcctccgcg gccaggccgt cccggggagc cggtgggatg
      181 gacacctcca cccgttcccc cggctgcagc cggtaggcgg ccttcacgac ccgtccgccc
      241 accacgaccc gccccgcctc gatcagcccc ttcacccgca tccgcgacag gccttccagc
      301 cggcccgcca gccacgcgtc cagccgcgcg cctgcctccg ccgcttccac gaccccgcgc
      361 cagtcccggg ggagatcctt ctccgattcg ctcatcagga cccggccgct gcccgggcaa
      421 ccgcgccgtg cacggagcgg ctcagcgagc gggggagggc ggcgccgtga gcatgcgcag
      481 cgcgagcagc acgaccccga cggagatcga cgcgtccgcc acgttgaagg ccggccagtg
      541 gtatccgcgc cagtagaagt cgaggaagtc caccaccgcg ccgaaccgcg cgcggtcgat
      601 caggttgccg accgcgccgc cgaagatgag ccccagggcc agcggggtga gccgcccgcc
      661 cgtcggcagc atgcgcaggc ccacgacggc cagcaccgcg agcgcgccga tcgacagcag
      721 ggcgaccagc cagcgccagc ccgcgggcgt gcccgcgagc atcccgaaag cgaggccggg
      781 attcatcacg agggtgaggg agaagagccc gtcgatcacg tggaccgggc ggccgggggg
      841 caggcgctcg agcgccaggt acttggtcag caggtcgagg acgaggacga cgagcgcggt
      901 gaggccggcc gacttcaccg ctggagcgcc gtcacgaccg gggcgcagcg ctcgcagagc
      961 gtcgggtgcg cggggtcctc gccgacacgc gggctccagg tccagcagcg ctcgcacttc
     1021 ttccagccga gggtctgcgc gggcacgatc tcgaggacga ggccggggat gtcctggctc
     1081 tcgtaccgca ccgcctcccc cggggcccgc tgcttgagcc gcgtccccga aacgttgaag
     1141 agggtggtga gcagcgcttg gcccttggcc tcgagcagcg ggagccactg ctcctcgggg
     1201 gcgctcggga tgtagaggac cgcgtcgatg cccttgccaa tcctcccctg ctgccgcgcg
     1261 gcctcgagcg cgcgcgagac ctcgccgcgc acctcgagca gccgctccca gtccgcgctg
     1321 aggcgctcgt tcagccactc cccgcgctcg tcggggaaga gcaccaggtg cacgctctcc
     1381 ggcttgccgc gccccgggat gtgggaccag acctcgtcgg atgtgaaggt caggatcggc
     1441 gccatgagcc gcgtcaacgc ggtcagcacc tcgaagcaga cggtctggga ggcgcgccgg
     1501 cgcgagtcgt ccggggccga gacgtagagc cggtccttga tgatgtcgag gtaaagcgac
     1561 gagagatcca ccgcgcaaaa gttgtggatg gcgtggaagg cgacgtggaa ctggtagtcc
     1621 tcgtacgccc ggcgcacccg cgcgatcagc ccgcccagcc gcagcagggc ccagtggtcg
     1681 agctcgtcca tgtcgccgta cggccgccgg tcgcgctccg ggtcgaagtc ggccagagtg
     1741 ccgaggagga agcgggaggt gttccggatg cgccggtagg cgtcggcgag ccggttcagg
     1801 atctcgtgcg agagccggat gtcctcggac gaatcctcgg cggccaccca caggcgcagg
     1861 acctccgcgc cgtacttcgg gatgagctca tcgggggaga tgttgttgcc ccccgacttg
     1921 gacatcttcc gtccctcgcc gtccaccacg aagccgtggg tgaggacgga gcgatagggc
     1981 ggcttgtcgc gcgtccccac cgcttccagc agcgaggagt gaaaccagcc gcgatgctgg
     2041 tccgagccct cgagatacat ctcggcgggc cagcgcagct ccggccgggt ctccagcacc
     2101 gcagcgtggc tgcagccgga gtcgaaccac acgtcgagga tgtcggtctc cttgcggaac
     2161 tccccgcccc cgcacctggg gcagcgcgtg cccgcgggca ggagcgccgc cgcctcccgc
     2221 gtgtaccatt catccgcgcc cgcgccgtcg cgcatgatcc cggccacgtg ggcgaccagc
     2281 gcctcgtcga ggagcagccc gtcgcagctc gcgcagtaga aggcgatgat gggcacgccc
     2341 cacacgcgct ggcgggagat gacccactcg ggccggtgcg ccaccatgtt gtagatgcgc
     2401 tcctcgcccc acccggggat ccacgtcacc tcgttccgga tcgcgtcgag cgccttctgg
     2461 cggagcccgt ggcggtccag cgcgatgaac cactgctcgg tggcgcggaa cagcgtcggg
     2521 ttcttgcagc gccagcagtg cgggtaggtg tgctggagcg ggacctgggc cacgagggcg
     2581 ccggccgcgc gcatgtgctc gatgatgcgc gggttcgcct cccagacggt ctggcccgcg
     2641 aaggaggcca cctcggcgat gaagcgcccg tcgtcgtcca ccgggttgta gatcttgagc
     2701 ccggcccggc ggccgagctc gtagtcttcc tcaccgtggc cgggcgcgat gtgcaccagg
     2761 ccggtgcccg cgtccatcgc cacgaagtcc gccgtgagca ccggcacgtc gcgatcgatc
     2821 cacgggtgcc gggcgaccat cccctcgagc gtccgccccg gcaccgcgag cgggagcgcg
     2881 gcgccccgcc ccttcacacc gggcaggcgc aggaacgcct ccagcaaggg ctgggcgacg
     2941 atgagggcct ggccgtcgag ctccaccgcg gtataggtct cgccggggtg gacggcgatc
     3001 gcgaggttcg cgggcagggt ccacggcgtg gtcgtccaga tcacgagcga caccggccgc
     3061 ccaccgaccg cgcgggcgat ctccgcgggg accgtctgga ccgggaattt cacgtagacc
     3121 gagggcgtgg tctggtcctc gtactccacc tcggcctggg ccagcgcggt cttgcagtgc
     3181 atgcaccagt gcacgggctt gagccccttg tgcactaggt cgcggcccac gaaccggccg
     3241 aactcgcgcg cgatcaccgc ctggtacgcg ggcgccatcg tccggtacgg atcgttccag
     3301 tcgccgaaca cgccgagccg ccggaactcc tcgcgctgga tgtcgatgaa cttggacgcg
     3361 tactcgcgac agcggcggcg cttctccatc gggtccatgg cctggcgcac gtccacgctc
     3421 gcctcgtcga gacccagctc cttgtccacc tggtgctcga tcggcagccc gtggcagtcc
     3481 cacccgggca catagaccgc gttgttgccc agcatggagc gcgatttcac gacgagatcc
     3541 ttgaggatct tgttcagcgc cgtgcccatg tggatgtggc cgttggcgta gggcggccca
     3601 tcgtggagga tccacagcgg acggctcgcc gaggcctgcc ggatccgccc atagatgtcc
     3661 atcgccgccc agcgctcgag ccattccggc tccatcttgg gcaggttggc cttcatggga
     3721 aacgcggtct tgggcaggtt tagcgtgtcc ttgtaatcca tagagggcca aagctaacac
     3781 tcatcagggg tcccggcaag aacggggccc cgggagcggg ccgacggcga gttacttctt
     3841 gggggtgagc gtgacgacgc gggcgacgct gtcgatggtg acgatgaagt tgtcgagaaa
     3901 gtcgcggccg agcaggccgt cgcggcccga gccgaggccg gaatcgtgcg agacgaccat
     3961 caacgggccg aaccgggcgc cttccacctc gatgctttcg atcctcaccg cgaggacgtt
     4021 ggcgtcgccg gtcacgccct tgatggagcc gcgcacggca ttgcggtagc tgatacccag
     4081 cgcggccagg gcggtcgggc tgatcagcgt cccctgggcc cccgtatcga ggatgagctg
     4141 agtggtgccg ccgccgttga tcttggcgga caccacgatc ggctggccgg gcgtgaacgg
     4201 gatgcgggcc acccccgcgg gctgagcgct cggcgcggac ggctcgggcg tggtaggcgc
     4261 gggctggctc ggcgcggcca ccatcaccgc cgccccgcgg aactgcggcg gcacgctgtg
     4321 gatgccctgg gagaagcgca cctccccccg ctcatccgtc cagcggtaga tctgagcgga
     4381 cgcgggcgtg atcgcacccg acagcaagct gccgagcgcc aggcccatcg cgaggacggg
     4441 gtggagaccc ttcatggata cctcgtggca attatacggt tcccgggaat ttccgcgggc
     4501 gtctcccgtg gagtccccag gccccgggcc aaggctcccg gacacggccg gcggtccggg
     4561 cgccaccggc tccaggcggt ctcgtaggcc accagctcat cggcccccgc cacctcgttg
     4621 tgatcgagcc ggacgcggta cacgggatgg aagcggtcgg ccatctccga gatcatcggg
     4681 aaccaggccg ggaagacgat caggtagtcg gggcaggcct gctcgagata gcgcagcacg
     4741 cccatctcgc cgtcccggcg gaacgggatg atggcgggcg tgaccaggcc catcacgtcc
     4801 acgacctcgc ggcgcgagat gtaggcgatc gcaccgacgt cgttcagtcc gatccgcgcc
     4861 gtgggtggcg tgtggcgcgc gacccagtga ccgagatgca cctgcatcgt ctcgatgttc
     4921 tggaccgccc agccgtagcg cgacgccgcg gacggcaggg ccacgaccgc gcccacgagg
     4981 agcgcggcgg atgcgagcca tccgaggcgc cccctcagcc atcgcggcca tcccgggggg
     5041 gagagggcgg aggagaagag cggggtgagg gggacgacgg cgacgacgag cgcgagcgga
     5101 aggaggtgga tgcaatagcg tccctcctga aaggatgggg cgcggtacgg cgcgagcatg
     5161 gccatcgcga gcgggtggag cacgagcacg gaggcgggcg ccagcgcggc gcggccgacg
     5221 tggcgaccga gccaccagag cccgggcacg agcaggagcg gcaggagcgt gtccacccgc
     5281 cagagccagc gcgcccattc cgcctcgaac tgccacggcc gctgcaggag cgtcatgcgg
     5341 agcggctcgc gcacgcccgc gagatagccg acgaggccgc cctcgatctt cgccgccgcg
     5401 gtggcgggga gcggggtccc ggtcgtcgcc aggttgaagg cgacccaggg cgccaggaat
     5461 cccgcgaccg gaagacccca cgccagggcg cgccggcccg tgagtgggcc ggccagccag
     5521 aagagcggca gcagcagggc ggcctcgggg cgcgcgagcg ccgcaagccc gagcacggcg
     5581 gcggccgccc aggtgcgacc gcgcgcgtgc agcaggagcg cggcggtcac cagcatggcg
     5641 gccagcgcaa cctcc
//
LOCUS       DASUFA010000252         8737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_737940, whole genome shotgun sequence.
ACCESSION   DASUFA010000252 DASUFA010000000
VERSION     DASUFA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737940"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2672)
                     /locus_tag="VFX87_09370"
     CDS             complement(<1..2672)
                     /locus_tag="VFX87_09370"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX4995167.1"
                     /translation="MTRIRRSKGIVAGAIAGLLVLCGGLAAWAQAPKADRPTYAVGDK
                     WIRSDGAYDLIRIENARYIFAADGGREVHLTRDLGVAKIVRGGQVLLELEPPPGLTWP
                     LEVGQSGVSWLTLKSLDPQFGQSLVRLTWKVDAYQDVRVPAGVFKAFRIVQVLEPRFL
                     AVSPQSRRLELAFWYAPAVQQLVRADGNDLSGLAFQAVALDRPAAAALAVALADPKDQ
                     GRVATERITLTGKVTGGTGPLRIAVTLNGTEIARQQEPGTPGKDVALSVPVKLAPGRN
                     MLIVTATDGAGVSRQQGRVLFYDKPAPAVAAAPTAPPAPARPTPPASAIPPPVIAPAA
                     PSAATAPPVTAPPPLAVTPMPTPVPPPAAAPPPTSVPAPARPPVVAAPAPAPAPREPS
                     RPTAEPPRAPTPAPPVASPVPAAPAPPPVVAAVPAEASAKPSAVFRITISTPANQARV
                     EQETVTFAAVVYGGTGVRQVLVTANGVELWRQDNRTQQPSMAVNLPVKLAEGQNTLVV
                     TAAETDGTMHQEMRTIHHEKLMPLAVDVRYPEDRSRVTDEASVVAAVAQSSKGISRIT
                     VTLNGTEVHQQEERSPQKTMAVSAPLTLREGANAIVITAREPDGAARQEVRTVILERA
                     RPAAPVAPPAPPAAPPSTQWAVIIGVGGYESSAVPRLRYSVADADAVYQTLISAGFKK
                     ENILLLTDKTERKPTLRNIKWALGTFLARSAHKDDLVMIYFAGHGASEVDQRGIERDG
                     LSKYLVPVDADPDDLYSTALPMDEMQNVLARIEAERITVFLDACYSGAAGGRTFASTK
                     TRTVNVDDIFLDRLTRSKGRAIVTASRPSELSIELAELGHGIFTYYLVRGLQGYADNN
                     RDGIVSLQELYEYLAQEVSRKSRQVGGNQHPMLKG"
     gene            2812..3819
                     /locus_tag="VFX87_09375"
     CDS             2812..3819
                     /locus_tag="VFX87_09375"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX4995168.1"
                     /translation="MRAINSLAFRWPMVALSLGLVAGCADLQVKEAEEASRSRVFVAN
                     ESSNSVTVVDADSFQVVATVDARNHATHDLAVSRDGRWLFATNLASGRLSAISTRTLE
                     TVASIATGDRAHVVTLTNDNRQAWVANIGENTISIVDTSTYRILGTIAVGKGPTGLTF
                     SRDGRFAYVSNQGDKTVEVIDTGSHRVLKAIPVGTNPHFLVLGPDGRIWGTNTGGTDI
                     YVIDPATQDKVGTINVGPAPQQIAFGFKGLQGPNAYVTVAGLNRVVVVSADPSNLRIL
                     EQIDVGQRPNGISSNREGTRVFVVHENSNDLKVIDTGSSSVLATVPVGRKPIRVVYHG
                     N"
     gene            3860..4465
                     /locus_tag="VFX87_09380"
     CDS             3860..4465
                     /locus_tag="VFX87_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX4995169.1"
                     /translation="MIALALWSLLPVAGVRPAAAQGVPVGTAQNAVGTLVVVRTDGIE
                     HRLQGRGSLPLFEGDVLRTEAASQALIQLRPGIPVALNERTAVKILSRWDKTGGTTRI
                     VRVSRGGIWAKAGSGASQLEIETPAAVAAARDAEVDLKVAEDGQSSLTVVQGTAELAT
                     PFGVCAVRGGTASVGARGAACTAPQPANVAGAVSWKQAVTQ"
     gene            complement(4491..4682)
                     /locus_tag="VFX87_09385"
     CDS             complement(4491..4682)
                     /locus_tag="VFX87_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995170.1"
                     /translation="MEDSVALFDTIILVAGIALILLTSLRVSRQRAGRKPVKGQTGAL
                     VGIVVWLGCLGVALYLIFG"
     gene            complement(4729..4920)
                     /locus_tag="VFX87_09390"
     CDS             complement(4729..4920)
                     /locus_tag="VFX87_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995171.1"
                     /translation="MADGSPLGVVARLERRYTFPDGSTGPAYVIRSTDQQESAFDADE
                     LERGARIEAIRDVRIPPTR"
     gene            5526..5762
                     /locus_tag="VFX87_09395"
     CDS             5526..5762
                     /locus_tag="VFX87_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995172.1"
                     /translation="MDTPASEQGSSLETVMKRGAVVCVVALGGVAVGLAVWAIRRWQD
                     EREYQAWRESVTADPYRRDRNGVPVGAQLGYSRR"
     gene            5858..6688
                     /locus_tag="VFX87_09400"
     CDS             5858..6688
                     /locus_tag="VFX87_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEX4995173.1"
                     /translation="MTRPERDPAAPGRFTMSSEASHLARLREWLREELAGLGVERETR
                     AALVLAVGELCANSIEHAYEGRGGEPIHVSIRGFEDRLVLEVEDFGRAFDAARYVEPD
                     LDALPDHGLGIHLVHRIADSVSVDIRRQRGTRWTLVKYRPGHGPAARATAAGGGAPPA
                     RSGEVMDIEVTRSAAITVVVPKGDLDMAAADQMKRVLAGLVSEGSVKLLVDLGNVGYI
                     DSSGMGALVATLKVARAAGGDLHLCALQDDVRAIFEMTRLIKAITIHATRGEALAAWV
                     "
     gene            6679..7548
                     /locus_tag="VFX87_09405"
     CDS             6679..7548
                     /locus_tag="VFX87_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HEX4995174.1"
                     /translation="MGLTRRLLAAALLLGLGGASAPAASPSRPLAGVRTWVVYYAATP
                     EAAADLARFDVVVLDPHAHPPLPLVKRHGSLLLSYVSLGEVNTSHPEYTAIASEPWVL
                     SPNPSWPDARRLDVRAPAYERWLLDRVVPPALAGPVNGLFLDTADTVLDLERTDPRRF
                     KGAAQALERVLVEMKRRNPRALLMINGGLPIAERLPTVLDGVALESIWTDYDFKAKAY
                     RVRPADEAESRALLLARVVALGLPGFTLEYTAAAHGTAWPAELIRRSRARGFIPYVAT
                     IGLDQVSTHTLAP"
     gene            7556..>8737
                     /locus_tag="VFX87_09410"
     CDS             7556..>8737
                     /locus_tag="VFX87_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995175.1"
                     /translation="MTRKLSRRALLGRSAAAAGVLALRAGGARAPAAQAAPGPAEPGE
                     MPRYVLALYKSSELSNDVDGHRPKTATLNEVHSWAQMPLNWLGLMVEYHDIDRGLPDE
                     RTMSRYRGVITWFQSEEMAAPLEYLRWLGAQIRAGRRVVILEYLGALRDRRTLRTVDL
                     TAVNEALAPSGLEFLGNWTGNQKVIELRRKDPKMMEFERPLPPGLPYYQQVRARRPDS
                     HAHLVLARRDLPDSESHVVVTGPWGGFAAAEYVRFQLPGESGTQWWLDPFAFFREALG
                     VGDWPRPDTTTQCGRRICYSHIDGDGLRNRSEVRPGVSSGQILLDEILSRYGLPTSVS
                     VVVAEVEPTLLGSPEFHELARAMFRLPNVEAGSHSYTHPLDWEKRTRSFTPAGIPYSV
                     ET"
BASE COUNT         1216 a         2996 c         3246 g         1279 t
ORIGIN      
        1 cccttcagca tgggatgctg gttgccgccc acctggcgcg acttgcggct gacttcctgg
       61 gccaggtact cgtagagctc ctgcagcgag acgatcccgt cgcggttgtt gtccgcgtag
      121 ccctgcagac cgcgcaccag gtagtaggtg aagatgccgt ggcccagctc ggccagctcg
      181 atggacagct cggacggccg tgaggcggtc acgatcgcgc ggcccttgga gcgcgtgagc
      241 cggtcgagga agatgtcgtc cacgttgacg gtgcgcgtct tggtggaggc gaaggtgcgc
      301 ccgcccgccg cgccgctgta gcaggcgtcg aggaacacgg tgatccgctc cgcctcgatc
      361 cgggccagca cgttctgcat ctcgtccatc ggcagcgcgg tcgagtagag atcgtcgggg
      421 tcggcgtcca ccggcaccag gtacttggag agcccgtcgc gctcgatgcc gcgctggtcc
      481 acctcggagg cgccgtggcc cgcgaaatag atcatcacca ggtcgtcctt gtgggccgac
      541 cgggcgagga aggtgccgag cgcccactta atgttgcgca gggtgggctt gcgctcggtc
      601 ttgtcggtga gcaggaggat attctccttc ttgaagccgg cgctgatcag cgtctggtag
      661 acggcgtccg cgtcggccac cgagtagcgc aggcgcggca ccgccgagct ctcgtagccg
      721 cccaccccga tgatgaccgc ccactgcgtg gagggcggcg cggcgggcgg ggccgggggc
      781 gccacggggg cggcgggcct ggcgcgctcc aggatcaccg tgcgcacctc ctgccgggcc
      841 gcaccgtcgg gctcgcgcgc ggtgatcacg atggcgttcg cgccctcgcg gagcgtgagg
      901 ggcgcgctca ccgccatcgt cttctgcggg ctccgctcct cctgctgatg cacctcggtc
      961 ccgttcagcg tgaccgtgat ccggctgatg cccttgctcg attgcgcgac cgcggccacc
     1021 acgctcgcct cgtcggtgac gcgcgagcga tcctccgggt agcgcacgtc caccgcgagc
     1081 ggcatcagct tctcgtggtg gatcgtgcgc atctcctggt gcatcgtgcc gtccgtctcg
     1141 gccgcggtga ccaccagggt gttttggccc tccgccagct tgaccgggag gttcaccgcc
     1201 atggacggct gctgcgtgcg attgtcctgg cgccagagct ccacgccgtt ggcggtgacg
     1261 aggacctggc ggacgcccgt gccgccgtac accacggccg cgaacgtgac cgtctcctgc
     1321 tccacgcgcg cctggttcgc gggtgtggag atcgtgatcc ggaacacggc cgagggtttc
     1381 gccgatgcct cggccggcac ggccgcgacg accggcggtg gcgcgggcgc ggccggcacg
     1441 ggcgatgcca ccgggggggc cggagtcggg gcgcgcggcg gctcggcggt cgggcggctc
     1501 ggctcacgcg gcgcgggtgc cggcgcgggc gcggccacga cgggtggcct ggccggagcc
     1561 ggcacgctgg tcggcggcgg tgccgcggct ggcggcggca cgggcgtcgg catcggcgtg
     1621 accgccagag gcggcggggc ggtcaccggc ggcgcggtcg ccgcggatgg cgcggccggc
     1681 gcgatgacgg gcggcgggat cgcgctcgcc ggcggcgtgg gccgcgccgg ggctggcggc
     1741 gcggtcgggg ccgcggccac cgcgggcgcg ggcttgtcgt agaagagcac gcgcccttgc
     1801 tggcggctga cgccggcgcc gtccgtcgcg gtgacgatca gcatgttccg gcccggcgcg
     1861 agcttcacgg gcacgctcag cgcgacgtcc ttgccgggcg tgccgggctc ctgctgccgc
     1921 gcgatctcgg tgccgttgag cgtgaccgcg atccgcagcg ggccggtgcc gcccgtcacc
     1981 ttgccggtca gcgtgatccg ctccgtggcc acgcggccct ggtccttcgg gtcggcgagc
     2041 gccaccgcca gcgccgccgc cgcgggccgg tcgagggcga cggcctggaa ggcgagcccg
     2101 ctcagatcgt tgccgtcggc ccgcacgagc tgctggacgg ccggcgcgta ccagaacgcg
     2161 agctcgagcc gccgggactg gggtgagacg gcgaggaagc gcggctcgag cacctgcacg
     2221 atgcggaagg ccttgaacac cccggcgggc acgcgcacgt cctggtaggc gtccaccttc
     2281 caggtgagtc ggacgaggct ctggccgaac tgcgggtcga ggctcttgag cgtgagccac
     2341 gacaccccgg actggccgac ctcgagcggc caggtcaggc cgggcggcgg ctcgagctcg
     2401 agcagcacct ggccgccgcg gacgatcttg gccacgccca ggtcgcgggt gaggtggacc
     2461 tcgcggcccc cgtcggccgc gaagatgtag cgggcgttct cgatccggat gaggtcgtag
     2521 gcgccgtcgc tgcggatcca cttgtcgccg accgcgtagg tgggccggtc ggctttcggg
     2581 gcctgggccc acgcggcgag gccgccgcac agcacgagga ggccggcgat cgcgcccgcg
     2641 acgatgccct tactgcgtcg tatccgcgtc atgggaatag cgttttctcc gctcggcctg
     2701 ggttagagac taagcccttc tccggagggc tgtcaacgaa cgggtcttga ctcgccgggg
     2761 ttcctggacc aaggtagggg gcgctttccc agccgaaatc aggaggagtc catgcgcgcg
     2821 atcaactcgc tagcgttcag gtggccgatg gtcgctctct cgctcgggct cgtcgccggc
     2881 tgtgccgatc tgcaggtgaa ggaggccgag gaggcgtccc gctcgcgggt cttcgtcgcc
     2941 aacgagagct cgaactcggt gacggtggtg gacgccgaca gcttccaggt cgttgccacc
     3001 gtggacgcgc gcaaccacgc cacccacgac ctggcggtct cgcgcgacgg tcgctggctc
     3061 ttcgccacca atctggcatc ggggcggctg tcggccatca gcacccgcac gctcgagacg
     3121 gtggcgtcga tcgccaccgg tgaccgggcc cacgtcgtca cgctcaccaa cgacaaccgg
     3181 caggcgtggg tggccaatat cggcgagaac acgatctcga tcgtggacac cagcacgtac
     3241 cggatcctcg gcaccatcgc ggtgggcaag gggccgaccg ggctcacctt ctcgcgcgac
     3301 ggccgcttcg cctacgtgag caaccagggc gacaagacgg tggaggtgat cgacaccggc
     3361 tcgcaccgcg tgctgaaggc gatcccggtg ggcaccaacc cgcacttcct ggtgctgggc
     3421 cccgacgggc gcatctgggg cacgaacacc gggggcaccg acatctatgt catcgatccg
     3481 gccacgcagg acaaggtcgg caccatcaac gtggggccgg cgccgcaaca gatcgcgttc
     3541 gggttcaagg gcctgcaggg cccgaacgcc tacgtcaccg tcgcggggct caatcgcgtg
     3601 gtggtggtga gcgcggatcc gagcaatctc cgtatcctgg agcagatcga cgtggggcag
     3661 cggccgaacg ggatctccag caatcgcgag ggaacacgag tcttcgtggt gcacgagaac
     3721 tcgaatgacc tgaaggtcat cgacaccggc agcagctcgg tcctcgccac ggtgccggtg
     3781 gggcgcaagc cgatccgcgt cgtctatcac gggaactgag gcggccatgc gtgcgcgggc
     3841 cggatggagt ggaggggtgg tgatcgcgct ggcgctctgg tcgctgctgc cggttgccgg
     3901 cgtgaggccg gcggccgctc agggcgtgcc ggtcggcacg gcccagaacg cggtggggac
     3961 gctggtggtg gtgcggaccg acggcatcga gcatcggctg caggggcgcg gatcgctgcc
     4021 cctcttcgag ggcgacgtgc tccgcaccga agcggccagc caggcgctga tccagctccg
     4081 gcccggcata cccgtcgccc tgaacgagcg cacggcggtc aagatcctct cgcgctggga
     4141 caagacgggc gggacgacgc gcatcgtcag ggtgagccgg ggcgggatct gggccaaggc
     4201 ggggtccggc gcgagccagc tcgagatcga gactcccgcc gccgtcgccg cggcgcgcga
     4261 cgccgaggtc gatctgaagg tggccgagga cggccagagc tcgctcaccg tggtgcaggg
     4321 caccgcggag ctggccacgc ccttcggcgt ctgcgcggta cgagggggta ccgccagcgt
     4381 gggagcgcgc ggcgcggcgt gcacggcccc gcagccggcg aacgtggcgg gcgcggtgtc
     4441 ctggaagcag gcggtgacgc agtaggcctc agccgaaggt cagccgaaga tcagccgaag
     4501 atcagataga gcgccacccc gaggcacccg agccagacca cgatgccgac gagggcgccg
     4561 gtctgcccct tgacgggctt tctgccggcc cgctggcggg agacgcggag gctggtgagc
     4621 aggatcaggg cgattcccgc taccaggatg atcgtgtcga acagggctac ggagtcttcc
     4681 acccggactc tacccttgtg gtggcctcgc ggtggggcgg gctctcggct agcgtgtcgg
     4741 gggaatacga acgtcccgga tcgcctcgat gcgcgcgcct cgctccagct cgtcggcgtc
     4801 gaaggcgctc tcctgctgat cggtcgaccg gatcacgtag gcggggccgg tggagccgtc
     4861 gggaaacgtg tatcgccgct cgagtcgcgc gacgacgccc agcgggcttc catccgccat
     4921 gcggctcaag gtatcaccgg gcctgaagaa gaacgcccat tcggcctgcc cccgcgagcg
     4981 ggagacgacc ggtgcgggcg attccgttgc cggcggggcg gcgggcgccg agacgacctc
     5041 cgcaggttgt gactcggcta cgggccgtgg aggttcgcgc cgcccggata tgagggtgac
     5101 gacggtgatc gccaccagca gtccgagaag cgcgcccccg agcatcttga ggcgggatcc
     5161 ggccacgaca gtcatgacgg aaagggagag gcaagaacag ggccagccgt ccgcacaata
     5221 attgcagaca cttagcgcgc atgggctgcg gcgtgtggcc aagtctgggc acccgacctg
     5281 acaacctccg gataccgagg ggcccgcggg accacaactg gctgatttgg cgcggcggcc
     5341 ggcatggctt gggtcttgct accttacccg ggcggacgaa agacgcgccc tatgaacgag
     5401 atccgggagg cccgagcgcg atgctgctga cgacgctgat cacggtggcg gtcatcggag
     5461 cgagctcgct catactctgg ctctcgatag gcgcggacta gaggcgaccg gaggggatgg
     5521 cggccatgga tacgcccgca tcggagcagg gtagctcgct ggagacggtg atgaagcgcg
     5581 gagcggtggt ttgcgtcgtg gcactgggtg gggtcgcggt gggcctcgcg gtgtgggcga
     5641 tccggcgctg gcaggacgag cgcgagtacc aggcctggcg cgaatcggtg accgcggatc
     5701 cctaccggcg cgaccgcaac ggcgttccgg tcggcgccca gctgggctac tcgcggcggt
     5761 agcacggccc cagcttcctc ggcgttccct ccggcgctca cgccagatga gcgccctccc
     5821 gctgctatac tgaatcgcgc gatgtgggcc ccagagcgtg acccggccag aacgtgatcc
     5881 ggcggcaccc ggccgcttca cgatgtcgag cgaggcctcg cacctggcgc ggctacgcga
     5941 gtggctgcgg gaggagctgg ccgggctggg cgtggagcgg gagacccggg cggctctcgt
     6001 gctggcggtg ggcgagttgt gcgccaactc catcgagcac gcctacgagg gccggggtgg
     6061 cgagcccatt cacgtctcga tccggggctt cgaggaccgg ctggtcctcg aggtggagga
     6121 cttcggacgg gccttcgacg ccgcgcggta tgtcgagccc gatctggacg ccctgccgga
     6181 ccacggcctg ggcatccatc tggtgcatcg catcgccgat tcggtttccg tcgacatacg
     6241 ccggcagcga gggacccgct ggaccctcgt caagtaccgg ccgggtcatg gcccggccgc
     6301 gcgcgcgacc gcggcgggcg gcggcgcgcc gccggctcga tcaggagagg tcatggacat
     6361 cgaggtgact cgttcggccg cgatcacggt ggtcgtcccc aagggcgatc tcgacatggc
     6421 ggctgcggat cagatgaagc gagtcctggc cggactcgtg agcgaggggt ccgtcaagct
     6481 gctggtggac ctcgggaacg tgggctacat cgacagctcg ggtatgggcg ccctcgtcgc
     6541 cacactcaag gtcgcccgcg ccgcgggcgg cgatctccac ctgtgcgcac tgcaggacga
     6601 cgtgcgggcg atcttcgaga tgacccgcct gatcaaggcc atcacgattc acgcaacccg
     6661 gggggaggcg ctggccgcgt gggtctgacg cggcgcctgc tcgccgccgc cctgctcctg
     6721 gggctcggcg gggcgtcggc gccggccgcc tcgccgagcc gtcccctggc gggcgtgcgc
     6781 acctgggtcg tctactacgc ggcgaccccc gaggcggccg cggacctggc gcgcttcgac
     6841 gtggtcgtgc tcgatccaca cgcgcacccg ccgctgcccc tcgtcaagcg gcacggctcg
     6901 ctcctgctga gctacgtgag cctgggcgag gtgaacacga gccatcccga gtacacggcc
     6961 atcgcgagtg agccgtgggt cctctctccc aacccgagct ggcccgacgc gcgccggctc
     7021 gacgtgcgcg cccccgcgta cgagcgctgg ttgctcgacc gggtggtgcc gcccgcgctc
     7081 gccggtccgg tcaacggcct cttcctcgat accgccgaca ccgtgctcga tctcgagcgg
     7141 acggatccgc ggcgcttcaa gggcgcggcc caggcgctcg agcgcgtgct cgtggagatg
     7201 aagcgccgca acccgcgggc gctgctcatg atcaacggcg gcctcccgat cgcggagcgg
     7261 ctccccacgg tgctggacgg ggtcgcgctc gagtccatct ggaccgacta cgacttcaag
     7321 gccaaggcct accgggtacg gccggccgac gaggcggagt cccgggcgct cctcctggcc
     7381 cgcgtggtcg cgctgggcct gccggggttc acgctcgagt acaccgcggc cgcccacggc
     7441 acggcctggc cggccgagct gatccggcgc tcgcgcgccc gggggttcat cccgtatgtc
     7501 gcgacgatcg ggctcgacca ggtctccacc cacaccttgg ctccttgacc cgcgcatgac
     7561 cagaaagctc tcgcgccgcg ccctgctcgg gcgctccgcc gccgcggcgg gtgtgctcgc
     7621 gctgcgggcg ggcggcgcgc gcgctcccgc ggcgcaggcc gccccgggcc cggccgagcc
     7681 tggagagatg ccgcgctacg tgctggccct ctacaagtcc agcgagctct ccaacgacgt
     7741 ggacggccac cggcccaaga ccgcgacgct caacgaggtg catagctggg cccagatgcc
     7801 gctcaactgg ctggggctca tggtcgagta ccacgacatc gaccgcgggc tgccggacga
     7861 gcggacgatg tcccgctatc gcggcgtcat cacctggttc cagagcgagg agatggccgc
     7921 gccgctcgag tacctccgct ggctgggcgc gcagatccgc gccgggcgcc gggtggtgat
     7981 cctcgagtat ctgggcgcgc tccgcgaccg ccggactctc cgaacggttg atctgacggc
     8041 ggtcaacgag gccctcgcgc cgagcgggct cgagttcctg gggaactgga cgggcaacca
     8101 gaaggtcatc gagctccgcc gcaaggatcc gaagatgatg gagttcgagc ggccgctccc
     8161 gcccggcctg ccctactacc agcaggtgcg cgcacgccgg cccgactccc acgcgcacct
     8221 cgtgctggcc cggcgcgacc tgccggactc cgagagccac gtggtggtga ccggcccgtg
     8281 gggcggcttc gcggcggccg agtacgttcg cttccagctc ccgggcgagt cgggcacgca
     8341 gtggtggctc gatccgttcg cgttcttccg cgaagccctg ggggtggggg actggccgcg
     8401 gccggacacc acgacccagt gcgggcggcg catctgctat tcgcacatcg acggcgacgg
     8461 cctgcgtaat cgctcggagg tgcgcccggg cgtctcgagc ggccagatcc tgctcgacga
     8521 gatcctttcg cgctacgggc tgcccaccag cgtgtcggtg gtggtggcgg aggtggagcc
     8581 gaccctcctg ggctcgcccg agttccacga gctggcccgc gccatgttcc ggctgccgaa
     8641 cgtggaggcg ggcagtcact cctatacgca tccgctcgac tgggagaagc ggacgcggag
     8701 cttcacgccg gccgggatcc cgtactcggt cgagacc
//
LOCUS       DASUFA010000253        10905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1556230, whole genome shotgun sequence.
ACCESSION   DASUFA010000253 DASUFA010000000
VERSION     DASUFA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556230"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFX87_09415"
     CDS             <1..243
                     /locus_tag="VFX87_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HEX4995176.1"
                     /translation="GRNMGTRHRAGLGLAEETDAVVLIVSEETGRISLAVDGQMESPL
                     DLDVLRRRLAELFSLEEAPLPRRSAWWEPAREWLRK"
     gene            240..617
                     /locus_tag="VFX87_09420"
     CDS             240..617
                     /locus_tag="VFX87_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX4995177.1"
                     /translation="MSVLTRHWELKLFALGVSMVLWAFVMTTEKSDLIMAAPLELDNI
                     PAGLEVKGDRPDSVDVQLHGLRGTLSRLGTDRVKARLNLAGSKAGEVTMRVLPEQITV
                     PPGITVVRVNPSRVRLVLGSPRS"
     gene            614..1963
                     /gene="glmM"
                     /locus_tag="VFX87_09425"
     CDS             614..1963
                     /gene="glmM"
                     /locus_tag="VFX87_09425"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX4995178.1"
                     /translation="MSRLFGTDGIRGLANTPPLTTELAFRLGRQLVATLLQHDGTKKT
                     RLVVGRDTRLSGPMLEGALVSGALSAGGDVYAVGVLPTPAIAFLTRRLEAHGGVVLSA
                     SHNSFEDNGIKIFSSEGSKFPDAWEEEIEGRLDGPDTAPWPTGVDIGRLVVHEAAEAD
                     YIAHARASCPHDLHDVRIVLDCAHGATYRVAPEIFRRLGADVRVLNASPDGQNINRGV
                     GAMHPEGLQKEVLRSRAQLGLAFDGDGDRLISVDETGQIRDGDYALAICGRHLAARGL
                     LKGGTVVTTVMANLALDRTLTKAGIGLAKVAVGDRYVLEEMLRIGANLGGEQSGHLLF
                     LDHATTGDGIVSALQLIGVMRESGASLGDLAQCLVKFPQVLVNVRVKAKPPVNTIPDL
                     AERARVLEAEMNGAGRILLRYSGTESLLRVMIEGEEQARIERMAEELAGIVRRAIGA"
     gene            1960..2373
                     /gene="acpS"
                     /locus_tag="VFX87_09430"
     CDS             1960..2373
                     /gene="acpS"
                     /locus_tag="VFX87_09430"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX4995179.1"
                     /translation="MSRVLGIGVDLTQMPRMRRVVARWDERFLQRVFTEQEIAYCRRR
                     RDPIPHFAARFAAKEATLKALGTGLRMGVNWRELEVRRERGQAPTMTLHGKSLAIALA
                     KGGRRVLLSLSHDGEYAMAQAMLIDDGADDEARPR"
     gene            2351..3145
                     /locus_tag="VFX87_09435"
     CDS             2351..3145
                     /locus_tag="VFX87_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995180.1"
                     /translation="MTRRGPGSLVVGLALLVQGCAGLIAGKDSAEAPAAPAISAPRTP
                     LPGGSVQVEKGRPGFVIAAPHGTSDIATDLMGRAVAKLTGFGLVVATGYANLDGEGRR
                     FNVNRPTESPPGAAARLEVETVAARQVYQDYRRHVTEAAQGAVRLYVEVHGNIRPESA
                     GRVEIATVGVSPNDAWRLKTLLELIRDSRLPPEGDVPRLDVWVESQDPVVYTASAAKQ
                     SGILALTPLALHIELPRMARTVHREAYTTVLADFLIQSAALLLPPR"
     gene            3150..4700
                     /locus_tag="VFX87_09440"
     CDS             3150..4700
                     /locus_tag="VFX87_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX4995181.1"
                     /translation="MLPVFTAAEMRALDARAIRELGIPGPLLMDHAGTGAAALIARWV
                     APIRGKAVAVVCGKGNNGGDGFVVARRLKARGAAVRVLLMGRRDEVRGDAADALGRWR
                     GRVEEIADPSGLARALGGADLIVDALLGTGVSGAARGPVAAAIEAINRAGADGVPVVA
                     LDLPSGLDSDRGALLGPTVKAWRTVTFAGLKRSLLLAPAAAQAGAVEIGDIGVPAEEV
                     RRDIGTWRLEAADVRPFFPPREPDAHKGRFGHLLVVAGSLGKTGAAVLAGRAALRSGV
                     GLCTIAAPASQQPIVAAQAPEYMTEALPETAAQSVSLEARDRILELARRMDAVGLGPG
                     LSLDRESQELARVLIRELDRPMVVDADALSALAGHLDLLRHAAGPRALTPHPGEMARM
                     LGTTIEIVQTDRIEVVRAFAREHGVAVALKGAYTVIAGPDGQVAINPTGNPGMAKGGS
                     GDVLTGIVGALLAREIEPAAALRAGCYAHGLAADVAVRERGEYGMLASDIIESLPAAL
                     RALAEGAA"
     gene            4697..5140
                     /gene="tsaE"
                     /locus_tag="VFX87_09445"
     CDS             4697..5140
                     /gene="tsaE"
                     /locus_tag="VFX87_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX4995182.1"
                     /translation="MSAPRTLISRSPEETRAIGEELGARLTAGAVVACTGELGAGKTC
                     FLQGLARGLGVTTDVTSPTFVLINQYRGRLPVYHLDAYRTGSLTELVDLGLEELLHGE
                     GVTVVEWADKLLPLLPARTIHVHLSGLGDEPREIRIEEPASDPGH"
     gene            complement(5552..5851)
                     /locus_tag="VFX87_09450"
     CDS             complement(5552..5851)
                     /locus_tag="VFX87_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995183.1"
                     /translation="MAVVALLGMACSTMTTEQRLRVDLFWEAARECDHFGTLSVTGID
                     VEGTVSLRANADSRAEFRPFMECFRQAVGARIERRRQAGEPIPPELLKEPTAEID"
     gene            5945..6643
                     /gene="radC"
                     /locus_tag="VFX87_09455"
     CDS             5945..6643
                     /gene="radC"
                     /locus_tag="VFX87_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX4995184.1"
                     /translation="MGAAGKGIGQWPAEDRPRERLYHKGPDSLADAELLAIQLGSGLA
                     GCSAMDMARDLLSRYGSLSGLAGCGVAELGAIRGVGPVKAVRLAATFEITRRLRSRNG
                     HGRVVLGSPEQVFARYGPLMEDLKKEVFRVALLDAQNGLLRDVVVSEGTLSASLVHPR
                     EVFKPAILESAASLILLHNHPSGDPTPSREDLRLTRQLAECAELLDMRIHDHVIIGRE
                     RFISLAQRGALTLR"
     gene            complement(6629..6871)
                     /locus_tag="VFX87_09460"
     CDS             complement(6629..6871)
                     /locus_tag="VFX87_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995185.1"
                     /translation="MEASRKPLAKVEGRKRMRLSGLTVAWRGTPSLDDWVAYIVTGTK
                     SKKLILADHASERKVKGLLTRIQGLSKKEVEKLAKG"
     gene            complement(7149..8156)
                     /locus_tag="VFX87_09465"
     CDS             complement(7149..8156)
                     /locus_tag="VFX87_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995186.1"
                     /translation="MRAEHDMVGAVDLLYRALYRGVLARMPERAAIAIGQWGLRHVPL
                     DRLGVFRNEDPRLAITLGGVRLPNPLILSSMYYDTAILRRAMGLGWGAVTAKSITPGP
                     RPGHPEPNLVRIETAEGPGLVNCNGFKNPGLEAYRQALVTLPHRVPLIVAAAGESADD
                     YVRVVAGLQEFGDLVEINISSPNTKLVYGWSTRPHELKDVFRAVRGATRKPVIVKISP
                     DFRETNEEITIPAALDAGITIVNYGNTRRVEEPRLSQRSGGLSGPALFATTLENVRRV
                     RERFGERLELIATGGIDSPESARQVLAAGATACGIFTGFITRGPLLARRILDALQPAE
                     K"
     gene            8171..8353
                     /locus_tag="VFX87_09470"
     CDS             8171..8353
                     /locus_tag="VFX87_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995187.1"
                     /translation="MPQDSTRRLLREFGIAVTAFEDAVEAGHGDDARKAEAALREHLK
                     GLIELVERLSEQAAKL"
     gene            8363..9124
                     /locus_tag="VFX87_09475"
     CDS             8363..9124
                     /locus_tag="VFX87_09475"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX4995188.1"
                     /translation="MPARRIVSLIPSVTEILFAIGAGDRVVGCTIYCTQPPEGVATKT
                     RIGGEKNPKLELIRELGADLVIANVEENVREHVETLRAWGIPVHVTYPRSVADGIRLV
                     GELGELLEAGSPARELEASLRARYDEVRAAAAGRRPRRVFCPIWRKPYMTVNADTYIH
                     DMLAVSGGSNVFGGAAKRYPEVTLDEVAATGAEVILLPDEPYRFRHVHQADFAPYPDL
                     PAVRDGRVHLVDGKLLSWYGPRIAEALRVLPPLLA"
     gene            complement(9121..9657)
                     /locus_tag="VFX87_09480"
     CDS             complement(9121..9657)
                     /locus_tag="VFX87_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4995189.1"
                     /translation="MGAEARIKELGITLPNPPKPMGNYIPAVRVGNLLFLSGHGPIRV
                     GDQPLTRGKVGRDMSTEDAYKVAREVGINLLGSARATLGSLDKVKRVVKVLGMVNAVE
                     SFGEQPKVINGFSDLMVEVFGENGRHARSAVGMGSLPAGIPVEIEMILELNASSGSAP
                     KKAARSARPAKKPAKRRR"
     gene            complement(9668..9928)
                     /locus_tag="VFX87_09485"
     CDS             complement(9668..9928)
                     /locus_tag="VFX87_09485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4995190.1"
                     /translation="MQEHRRYTRVAATWPVTVRSGDQILRLQTVNLSAHGVKVGLDAA
                     LPPVGTATHLRLEPPDAPPVDVQAIVWRADNDGSAFFFVGTS"
     gene            10004..10801
                     /locus_tag="VFX87_09490"
     CDS             10004..10801
                     /locus_tag="VFX87_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4995191.1"
                     /translation="MSPPAIPSPAATLVLLRDRPAGPAEILMIQRHSKSRFAAGDYVF
                     AGGKVEADDIPDDVERFIRGLSPEEASARIGGDLAPRKALGYWVGAIREAFEEVGMLL
                     AYDRQGELVRFTPENRERFEAYRAACQSANPAFFTMLRAEDLTLATDRLTYFAHWITP
                     EEQPLRFDTRFFGAVAPPQDPVVDGHEIVALRWLTPAEAMDAARRKEITLRLPTIKNL
                     ELVQGGGMRADEILGALRGRAVPTIRPRVLQVDGKPVPVLPGDPRWY"
BASE COUNT         1498 a         3816 c         3943 g         1648 t
ORIGIN      
        1 ggccggaaca tgggcacgcg gcaccgggcc ggcctgggcc tggccgagga gaccgacgcg
       61 gtggtgctga tcgtctccga ggagaccggg cgcatctcgc tcgcggtgga cggacagatg
      121 gaatcgccgc tcgacctcga cgtgctccgc cgccgtctgg ccgagctctt ttctctggaa
      181 gaagcgccgc tgccccggcg aagcgcctgg tgggagccgg cccgggagtg gcttcgcaaa
      241 tgagcgtcct cacgcgacac tgggagctca agctgttcgc tctgggcgtc tccatggtgc
      301 tctgggcctt cgtcatgacc accgagaagt cggatctcat catggcggcg ccgctcgagc
      361 tcgacaatat tccggccggg ctcgaggtga agggcgaccg gccggacagc gtggacgtgc
      421 agctgcacgg cctgcgcgga acgctgtcgc gcctcgggac cgatcgggtg aaggcgcggc
      481 tcaacctggc aggatcgaag gccggtgagg tgacgatgcg tgtgcttccc gagcagatca
      541 cggtgccgcc cggcatcacg gtggtccggg tgaatccctc ccgggtccgc ctcgtcctcg
      601 ggtccccccg ctcgtgagcc gcctcttcgg caccgacggt atccggggcc tcgccaacac
      661 gcccccgctg accaccgagc tggcgttccg cctcggacgg cagctggtgg ccaccctgct
      721 ccagcacgac ggcaccaaga agacgcgcct ggtcgtcggg cgggacacgc ggctctccgg
      781 ccccatgctc gagggcgcgc tggtctcggg cgcgctctcc gcgggcggcg acgtctacgc
      841 ggtcggcgtg ctgccgaccc cggcgatcgc gtttctcacc cgccgcctcg aggcccacgg
      901 gggcgtggtg ctgtcggcct cgcacaactc cttcgaggac aacggcatca agatcttctc
      961 ctcggagggc tccaagttcc cggacgcctg ggaagaggag atcgaggggc gcctggacgg
     1021 ccccgacacc gcgccgtggc ccaccggggt ggacatcggc cggctcgtcg tccacgaggc
     1081 ggccgaggcc gactacatcg cgcacgcgcg cgcgtcctgc ccgcatgacc tccatgacgt
     1141 gcggatcgtc ctcgactgcg cgcacggcgc gacctaccgg gtggcccccg agatcttccg
     1201 tcgtctgggc gccgacgtgc gggtgctgaa cgcgagcccc gacgggcaga acatcaaccg
     1261 cggcgtggga gccatgcatc cggaggggct gcagaaggag gtcctgcgct cgcgggccca
     1321 gctcgggctc gccttcgacg gcgacggcga ccggctcatc tcggtggacg agacgggcca
     1381 gatccgggac ggcgactacg cgctggccat ctgcgggcgg catctcgccg cgcgcgggct
     1441 gctcaagggc ggcaccgtgg tcaccaccgt catggccaat ctcgcgctcg accggacgct
     1501 gaccaaggcc ggcatcgggc tcgccaaggt cgcggtcggg gaccggtacg tgctggagga
     1561 gatgctgcgg atcggcgcca acctgggcgg cgagcagtcc ggccatctgc tcttcctcga
     1621 tcacgccacc accggggacg gtatcgtctc cgcgctgcag ctcatcgggg tcatgcgcga
     1681 gagcggcgcc tcgctgggcg acctggccca gtgcctggtg aagttccccc aggtgctcgt
     1741 caacgtgcgg gtgaaggcca agccgccggt caataccatt cccgatctcg cggagcgggc
     1801 gcgggtcctc gaggcggaga tgaacggggc ggggcggatc ctgctgcgct actccggcac
     1861 cgagtcgctc ctgcgcgtga tgatcgaggg cgaggagcag gcgcgcatcg agcgcatggc
     1921 cgaggagctg gcgggcatcg tgcgccgggc catcggggca tgagccgcgt gctgggcatc
     1981 ggggtggatc tcacccagat gccccggatg cggcgcgtgg tggcccgctg ggacgagcgc
     2041 ttccttcaac gggttttcac cgagcaagag atcgcgtatt gccggaggcg ccgcgatccc
     2101 atcccgcatt tcgcggcccg cttcgcggcc aaggaggcca cgctgaaggc gctcggcacc
     2161 gggcttcgca tgggcgtgaa ctggcgcgag ctcgaggtgc ggcgcgagcg ggggcaggcc
     2221 cccaccatga cgctccacgg caagagcctc gccatcgccc tcgccaaggg cggccgtcgg
     2281 gtgttgctct cgctcagcca cgacggcgag tacgcgatgg cgcaggcgat gctgatcgac
     2341 gatggtgccg atgacgaggc gaggccccgg tagtctcgtg gtcggcctcg ccctcctcgt
     2401 ccagggttgc gcggggctga tcgcggggaa ggattcggcc gaggcgccgg ccgcgcccgc
     2461 catctcggcg ccgcggactc cgctcccggg agggagcgtg caggtcgaga agggccggcc
     2521 ggggttcgtc atcgcggccc cgcacggcac ctccgacatc gccaccgatc tgatggggcg
     2581 tgcggtcgcg aagctcaccg gcttcggcct cgtcgtcgcc accggctacg ccaacctcga
     2641 tggcgagggc cgccgcttca acgtcaatcg cccgaccgag agcccgcccg gcgccgccgc
     2701 gcgcctcgag gtggagaccg tggccgcgcg ccaggtctac caggactatc gccgtcacgt
     2761 gaccgaggcg gcgcagggcg cggtccggct ctacgtggag gtgcacggca acatccgacc
     2821 cgagagcgcg ggccgcgtgg agatcgccac ggtgggcgtg agccccaacg atgcctggcg
     2881 gctcaagacc ctgctggagc tgatccgcga cagccgcctg ccgcccgagg gcgacgtgcc
     2941 gcgtctggac gtgtgggtgg aatcacagga cccggtggtc tacaccgcct ccgcggccaa
     3001 gcaatcgggc atcctggccc tgacgccgct cgcgctgcac atcgagctgc cgcgcatggc
     3061 ccggaccgtc caccgcgagg cctacacgac ggtgctcgcc gacttcctca tccagtcggc
     3121 ggctctgctg ctgccgcccc gctgacgccg tgctgcccgt cttcaccgcc gcggagatgc
     3181 gggcgctcga cgcgcgcgcc atccgcgagc tcggcattcc cgggccgctc ctcatggacc
     3241 atgccggcac cggcgcggcc gccctcatcg cccggtgggt cgcgccgatc cggggcaagg
     3301 cggtggccgt cgtgtgcggc aaaggcaaca atggtggcga cggcttcgtg gtcgcgcgcc
     3361 ggctcaaggc ccgcggggcg gcggtgcgcg tgctcctgat gggccggcgc gatgaggtgc
     3421 ggggcgacgc ggcggacgcg ctcggccgct ggcgcgggcg cgtcgaggag atcgcggacc
     3481 cgagcgggct cgcgcgcgcg ctcggcggcg ccgacctcat cgtggacgcg ctgctcggca
     3541 ccggcgtgag cggcgccgcg cgcgggccgg tggccgcggc catcgaggcc atcaaccggg
     3601 ccggcgcgga cggcgtgccg gtcgtcgcgc tcgacctgcc ctccggcctc gactcggatc
     3661 gcggcgcgct gttggggccc acggtgaagg cctggcggac cgtcaccttc gccggcctca
     3721 agcgaagcct gctgctggcg ccggcggccg cgcaggccgg cgccgtcgag atcggcgaca
     3781 tcggcgtgcc cgccgaggag gtgcgccggg acatcggcac ctggcgcctc gaggccgccg
     3841 acgtgcgccc gttctttccg ccgcgcgagc cggacgccca caagggccgc ttcggccacc
     3901 tgctggtggt cgcgggctcg ctcggcaaga ccggcgccgc cgtgctcgcg gggcgcgcgg
     3961 cgctgcggag cggcgttggc ctctgcacga tcgccgcgcc ggcctctcag cagccgatcg
     4021 tcgccgcgca ggcgccggag tacatgaccg aggccctgcc cgagacggcc gcgcagagcg
     4081 tgtcgctcga ggccagggac cgcatcctcg agctggctcg gcgcatggac gcggtcgggc
     4141 tcggccccgg gctctcgctc gatcgcgagt cgcaggagct ggcgcgggtg ctgatccgtg
     4201 agctcgaccg gccgatggtg gtggacgccg acgcgctgag cgccctggcc ggccacctcg
     4261 atctgctgcg ccacgccgcg ggcccgcgcg cgctcacccc gcatcccggt gagatggcgc
     4321 gcatgctggg caccacgatc gagatcgtgc agaccgaccg catcgaggtg gtgcgcgcgt
     4381 tcgcgcgcga gcacggcgtg gcggtcgcgc tgaagggcgc gtacaccgtg atcgccggac
     4441 cggacggcca ggtggcgatc aatcccaccg gcaatcccgg catggccaag ggcggctcgg
     4501 gcgacgtgct caccggcatc gtcggcgcgc tgctcgcccg cgagatcgag ccggcggccg
     4561 cgctgcgggc gggctgctat gcgcacggcc tggcggcgga cgtggcggtg cgcgagcggg
     4621 gcgagtacgg gatgctggcg agcgacatca tcgagagcct gcccgcggcg ctccgcgcgc
     4681 ttgcggaggg cgcggcgtga gcgcgccgcg gacgctcatc tcgcggagtc ccgaggagac
     4741 gcgggccatc ggcgaagagc tgggcgcgcg gctcaccgcg ggcgcggtgg tggcgtgcac
     4801 cggggagctg ggggcgggga agacctgctt cctccagggg ctcgcgcgcg ggctcggcgt
     4861 caccaccgac gtgacgagcc cgaccttcgt gctgatcaac cagtaccgcg gccggctgcc
     4921 ggtctaccac ctcgacgcct accgcaccgg cagcctcacc gagctggtgg acctggggct
     4981 cgaggagctg ttgcacggtg agggggtgac cgtggtggag tgggccgaca agctgctgcc
     5041 gctgctgccc gcgcgcacga tccacgtcca cctgagcggc ctcggcgacg agccgcgcga
     5101 gatccggatc gaggagcccg cttcagatcc gggacattga ccacttcgtc gccgcaggcg
     5161 gtacgcaact cttgcattat cttctattgg gagtagcctt gacgttgccg ctgtcgggag
     5221 tctctgaggg ctggtgtcga acggacatcg agacctcgac ggcgcggcgc ggagtggcct
     5281 gccgaaagtc ggggaggttc ccgatgatgg actcgcctac ctgtcccacc tgtgaccggc
     5341 ctatccgcct ggaggagtcc gtgtacttct ggcctgaccg tcggacgatg gagcatctct
     5401 ggtgtcacgt ccggtggttg aacatcgtcg ccgaggtcga agcctcggcg aatccggcct
     5461 atgggccaat gagctaaggc gtattcgcgt atacgcatct cgcgacctcc gcctgccatc
     5521 ctggaggcgc cctggtcgcg cgcggcgcgc gtcagtcgat ctcggcggtc ggctccttca
     5581 gcagctccgg cggtatcggc tcgcccgcct ggcgacgccg ctcgatgcgt gccccgacgg
     5641 cctggcggaa gcactccatg aacggccgga actcggcccg cgagtccgcg ttcgcgcgca
     5701 ggctgacggt tccttccacg tcgatcccgg tcacgctgag cgtcccgaag tggtcgcact
     5761 cgcgcgccgc ctcccagaag agatcgactc tcaggcgctg ctcggttgtc atcgtcgagc
     5821 aggccatccc gagcagcgcg acgacggcca cgacgatcgc gccggcccgt atggatcttc
     5881 gtccctgccc tgtacccgat ccggcgcatt gatccctcgc gcgccctcgt gctacgcgag
     5941 gactatgggc gccgcgggca agggaatcgg gcagtggccg gccgaggacc ggccccgcga
     6001 gcggctctat cacaaggggc ccgactcgct ggccgacgcg gagctcctgg ccattcagct
     6061 cggcagcggg ctcgccgggt gcagcgcgat ggacatggcg cgcgacctct tgagccgcta
     6121 cggatcgctc tcggggctgg ccgggtgcgg ggtggccgag ctcggcgcca tccgcggggt
     6181 ggggccggtg aaggcggtcc ggctggccgc gaccttcgag atcacccggc ggcttcggtc
     6241 gcggaacggc catggccggg tggtgctggg cagtccggag caggtcttcg cccgctacgg
     6301 tccactgatg gaggatctca agaaggaggt gtttcgggtg gcgctgctcg atgcccagaa
     6361 tggcctgctc cgggacgtgg tggtctcgga gggcacgctc tccgccagcc tggtgcatcc
     6421 gcgcgaggtc ttcaagccgg ccatcctgga gtcggcagcg tcgctgattc tcctgcacaa
     6481 ccacccgagc ggcgatccga cgcccagccg ggaggacctg cggctcacgc ggcagctcgc
     6541 ggagtgtgcc gagctgctcg acatgcgcat ccacgaccat gtcatcattg gccgcgagcg
     6601 gttcatcagc ctggcccagc gtggggccct aacccttcgc tagcttctcg acctccttct
     6661 tcgagaggcc ctggatgcgg gtgaggagcc ccttcacctt ccgctcggag gcgtggtccg
     6721 ccaggatcag cttcttcgac ttcgtacccg tcacgatgta ggccacccag tcgtcgagac
     6781 tcggcgtgcc gcgccacgcc actgtgagcc cgctgagccg catccgcttc ctgccctcga
     6841 ccttcgccag tggcttccgg ctggcctcca tacgtgatct tctccttctg aaaatgtgca
     6901 ctgaggcgat gagcgcccga tcaccttatg aaccggtccc agtcctgtca aggcttcgtt
     6961 tcgccgcgac tcatgcgtga ttcggcgccc gtggagccgc ccgactgcgt gaatgctgcg
     7021 tgtggtgatg ggtctcgtgg cggtggacga tcagagcgtc actttttcgt gccccgcctc
     7081 cggaaccgcg tcagagcgtg ccttttcctc gattgtggat cgctgtggat aagtggataa
     7141 cctgtgggtt atttttcggc gggttgcagc gcatcaagaa tacggcgcgc cagcagcggg
     7201 cctcgcgtga tgaatccggt gaagatcccg cacgcggtcg cgcccgccgc gaggacctgg
     7261 cgcgcgctct cggggctgtc gatgcccccg gtggcgatga gctcgaggcg ttcgccgaag
     7321 cgctcgcgca cccggcgcac gttctcgagc gtggtggcga acagggcggg gccggagagg
     7381 ccgcccgagc gctgtgacag gcggggctcc tcgacacggc gcgtattgcc gtagttgacg
     7441 atggtgatgc cggcgtcgag cgcggccggg atcgtgatct cctcgttggt ctcgcggaaa
     7501 tccggcgaga tcttgacgat caccggcttg cgggtggcgc cccgcacggc ccggaagacg
     7561 tccttcagct cgtgcggccg cgtggaccag ccgtagacca gcttggtgtt gggcgaggag
     7621 atgttgatct cgacgaggtc gccgaactcc tgcaggcccg ccaccacgcg cacgtagtcg
     7681 tcggccgatt cgcccgccgc cgccacgatg agcgggacgc ggtgcggcag ggtgaccagt
     7741 gcctgccggt aggcctcgag gcccgggttc ttgaagccgt tgcagttgac gagcccgggg
     7801 ccctccgcgg tctcgatgcg caccagattc ggctcggggt gcccgggacg cggccccggc
     7861 gtgatgctct tcgcggtcac cgcgccccag cccaggccca tggcgcggcg caggatcgcg
     7921 gtgtcgtagt acatggacga gaggatcagc ggattcggca gcctcacgcc gcccagcgtg
     7981 atggccaggc gggggtcctc gttgcggaag acgccgaggc gatcgagcgg aacgtgccgg
     8041 aggccccact ggccgatcgc gatcgcggcg cgctccggca tgcgggccag cacgccgcga
     8101 tacagcgcgc ggtagagaag atcgacggca ccgaccatgt cgtgctcagc gcgcatgata
     8161 tcctacaagc atgccgcaag acagcacgcg tcgactgctg agagagttcg ggatcgcggt
     8221 caccgccttc gaggacgccg tcgaggccgg gcacggtgac gatgcgcgga aggccgaggc
     8281 cgcgctgcgc gagcacctca aggggctgat cgagctcgtc gagcgcctgt ccgagcaggc
     8341 cgccaagctg taagccgcac cggtgccggc ccggcgcatc gtctctctca tccccagcgt
     8401 caccgagatc ctcttcgcca tcggcgccgg cgatcgcgtg gtgggctgca cgatctactg
     8461 cacgcagccg cccgagggcg tcgcgaccaa gacccggatc ggcggcgaga agaaccccaa
     8521 gctcgagctg atccgtgagc tgggcgcgga tctggtcatc gccaacgtcg aggagaacgt
     8581 gcgcgagcac gtggagacgc tgcgggcctg gggcatcccc gtccacgtca cctatccccg
     8641 gtcggtcgcc gacggcatcc ggctggtggg cgagctcggc gaattgctcg aggcgggatc
     8701 gccggcgcgc gagctggagg catccctgcg ggcgcgctac gacgaggtgc gggccgccgc
     8761 ggcggggcgg aggccgcggc gcgtgttctg cccgatctgg cggaagccct acatgaccgt
     8821 caacgccgat acctacatcc acgacatgct ggccgtgtcg ggcgggagca acgtcttcgg
     8881 tggcgccgcg aagcggtatc cggaggtcac gctggacgag gtcgcggcca cgggcgccga
     8941 ggtgatcctg ctcccggacg agccctaccg cttccgccac gtccaccagg ccgacttcgc
     9001 cccgtacccc gacctgcccg cggtgcgcga cggacgcgtg cacctcgtgg acggcaagct
     9061 tctgtcctgg tacggcccgc ggatcgcgga ggcgctgcgg gtgctgccgc cgctgctcgc
     9121 ctagcggcgg cgctttgccg gcttcttggc cggccgcgcc gagcgggccg ccttcttcgg
     9181 ggccgacccg gaggaggcgt tgagctcgag gatcatctcg atctcgaccg ggatgcccgc
     9241 ggggagggag cccatcccga cggcggagcg cgcgtgccgg ccattctcgc cgaacacctc
     9301 caccatcagg tcggagaagc cgttgatgac cttgggctgc tcgccgaagc tctcgaccgc
     9361 gttcaccatc cccaggacct tgacgacccg cttcaccttg tcgaggctgc ccagcgtcgc
     9421 ccgggccgag cccagcaggt tgatgccgac ttcgcgggcc accttgtagg cgtcctcggt
     9481 ggacatgtcg cggccgacct tgccgcgggt gagcggctgg tctccgacgc gaatcggtcc
     9541 gtggcccgac aggaagagca ggttgcccac gcgcacggcg gggatgtagt tgcccatcgg
     9601 cttcgggggg ttcgggaggg tgatgccgag ctccttgatc ctggcttctg cacccatgcg
     9661 ggtctcctca gctggtcccg acgaagaaga acgcggagcc gtcgttgtcg gcgcgccaga
     9721 caatggcctg gacgtcaacc ggcggcgcgt cgggcggctc gaggcggagg tgcgtggcgg
     9781 tgccgaccgg gggaagcgcg gcgtccagcc cgaccttgac gccgtgggcg ctcaggttca
     9841 cggtctggag acgcaggatc tggtctccgc tccggacggt gaccggccac gttgccgcca
     9901 cccgcgtgta ccgccgatgc tcctgcacga ccgggagttt cgcaccgcgg ttggggctcc
     9961 acaagggctt tttccgacgc ccccgcccgt gtcagaatgc gccatgagcc cgcccgcgat
    10021 tccctcgccc gccgcaaccc tggtcctgct gcgtgaccgc cccgccggcc cggccgagat
    10081 cctgatgatc cagcggcaca gcaagagcag gttcgcggcg ggggactacg tcttcgcggg
    10141 cggcaaggtc gaggccgacg acatccccga cgacgtcgag cgcttcatcc gcgggctcag
    10201 cccggaggag gcgagcgccc ggatcggggg cgatctcgcg ccccggaagg cgctcggcta
    10261 ctgggtgggc gccatccgcg aggccttcga ggaagtgggc atgctcctgg cctatgaccg
    10321 gcagggcgag ctggtgcgct tcaccccgga gaatcgcgag cgcttcgagg cgtaccgcgc
    10381 ggcctgccag tccgcgaacc cggccttctt caccatgctg cgcgcggagg atctcacgct
    10441 ggccaccgac cggctgacct acttcgcgca ctggatcacc cccgaggagc agccgctgcg
    10501 cttcgacacg cggttcttcg gcgcggtggc cccgccgcag gatccggtgg tggacgggca
    10561 cgagatcgtg gccctgcgct ggctgacgcc ggccgaagcc atggacgccg ccaggcgtaa
    10621 agagatcacg ctgcgcctgc ccaccatcaa gaatcttgaa ctcgttcagg gcggcggcat
    10681 gcgcgcggac gagatcctgg gcgcgctgcg cggccgcgcg gtgcccacca tccgcccgcg
    10741 tgtgctgcag gtggacggca agcccgtgcc ggtcctcccc ggcgatcccc gctggtacta
    10801 ggccgctggt actggcccgg ggggccctgc tatactcgcg gtccatcacc gctcgaggag
    10861 gccgttccga tgcgagccgg tcaggtatcc gtccagatcg cccct
//
LOCUS       DASUFA010000254         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1189662, whole genome shotgun sequence.
ACCESSION   DASUFA010000254 DASUFA010000000
VERSION     DASUFA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189662"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..979)
                     /locus_tag="VFX87_09495"
     CDS             complement(161..979)
                     /locus_tag="VFX87_09495"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX4995192.1"
                     /translation="MTPATYDLLVCDLDGTLLDDSLALDPALVEAFRGASERGLTISL
                     ATGRMPAAADSYRDQLGVTAPVIYYNGVVVRGGDGGPDLVSLTLPRGALRRAWAVFSQ
                     APVHPVFYRDDRLFCIAHTLAVRRYCEEEGLRVDVVDDAGEFLALGGFIKSLLIGHPK
                     DLDIVRGELTPIVAEHGRLVRTRGDYLEIIPVGASKGAALARLAAHLGVPLDRVVAVG
                     DQENDVEMLREAGLGVAMPHAPELVRRAADRIAPTAPEGGLLRLFRELFPGRFD"
     gene            complement(976..1224)
                     /locus_tag="VFX87_09500"
     CDS             complement(976..1224)
                     /locus_tag="VFX87_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995193.1"
                     /translation="MRALMLLVLIAVVALVVTRLPAAWRPRLPSPGERTRDELVKDPV
                     CQTYVLMSRAIRSEAGGAPLFFCSRECAARFQRGERRA"
     gene            complement(1233..1469)
                     /locus_tag="VFX87_09505"
     CDS             complement(1233..1469)
                     /locus_tag="VFX87_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995194.1"
                     /translation="MTQERPADRLMEKLLRAIEVSLKATEAAREAVQEILRRGAETGI
                     FFSGARAAESAEGFDLTEQDRDFLRSLSIRPDPR"
     gene            1508..2065
                     /locus_tag="VFX87_09510"
     CDS             1508..2065
                     /locus_tag="VFX87_09510"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4995195.1"
                     /translation="MAPDDTTRGWRFLRSTTRERLGKLSLREDVWRLPTGVEKTYPIL
                     HVGPTVGVVPFVDAHHVVLIRQFRHLLRGDSWELPGGGGQPGETPEAAAQRELQEEGG
                     YRAGRLTFLTRFVPSAAYLDETAYCFIGEELVADPLASDDDEFFERRVVPFRDAVAMA
                     LDDRITESVSKVALLAAALRRGLRP"
BASE COUNT          314 a          826 c          792 g          265 t
ORIGIN      
        1 accacgtgcc agccgggggc gatgcggcag aagcgcagga agcgccggcg cagccgcgcc
       61 tgggacggac tgcgcccgcg ggtctccatc atccgcgcga agcgctcggg ccggggctgg
      121 cggtcgacgg cccgccaggg atcgggatgc gggggcacgg tcagtcgaag cgaccgggaa
      181 acagctcgcg gaacagccgc aggagcccgc cctccggagc cgtcggcgcg atgcgatcgg
      241 cggcgcggcg cacgagctcg ggggcgtgcg gcatggccac gccgaggccc gcctcgcgca
      301 gcatctccac gtcgttctcc tgatcaccca ccgcgaccac ccgatcgagc ggcacgccca
      361 ggtgcgcggc gagccgcgcc agggccgcgc ccttcgaggc ccccaccggg atgatctcga
      421 ggtagtcgcc gcgggtgcgc acgaggcggc cgtgctcggc cacgatcggc gtcagctcgc
      481 cgcgcacgat gtcgagatcc ttcgggtggc cgatcaggag gctcttgatg aagccgccca
      541 gggccaggaa ctcgccggca tcgtccacca cgtccacgcg cagcccttcc tcttcgcagt
      601 accggcgcac cgccagggtg tgcgcgatgc agaagagccg gtcgtcgcgg tagaacaccg
      661 ggtgcaccgg ggcctgcgag aagaccgccc aggcccggcg gagcgcgccg cggggcagcg
      721 tgagcgagac gagatccgga ccgccgtcgc cgccccgcac caccacgccg ttgtagtaga
      781 tgaccggggc ggtgacgccg agctggtccc ggtacgaatc ggccgccgcg ggcatccgcc
      841 cggtggccag cgagatggtg agcccgcgct cggacgcccc gcggaaggcc tccaccagcg
      901 cgggatcgag cgccagggag tcgtcgagca gggtcccgtc gagatcacag acgaggagat
      961 cgtacgtcgc gggcgtcacg cccgccgctc tccccgctgg aaccgcgccg cgcactcgcg
     1021 gctgcagaag aagagcggcg ccccgcccgc ctcggaccgg atcgcccgcg acatcaggac
     1081 gtaggtctgg cacaccggat ccttcaccag ctcgtcgcgc gtccgctcgc cgggcgaggg
     1141 cagccggggc cgccacgcgg caggcagccg ggtgaccacg agggcgacca ccgcgatcag
     1201 gaccaggagc atcagcgcgc gcatcgcgcc tctcaacgag gatcgggtcg gatggagagc
     1261 gagcggagga agtcgcggtc ctgctcggtc agatcgaagc cctcggcgga ctcggcggcg
     1321 cgcgcgcccg agaagaagat gccggtctcc gccccgcgcc ggaggatctc ctgcaccgcc
     1381 tcgcgcgcgg cctcggtggc cttgagcgag acctcgatgg cgcggagcag cttctccatg
     1441 agccggtccg cggggcgctc ttgcgtcatg ggacttacga tagcacactg gcgcgacgga
     1501 gctttccgtg gcccccgacg acaccacccg cggctggcgc ttcctccgct cgaccacgcg
     1561 cgagcgcctc ggcaagctca gcctgcgcga ggacgtctgg cgcctgccca ccggcgtgga
     1621 gaagacctac cccatcctgc acgtcgggcc gacggtgggc gtggtgccgt tcgtggacgc
     1681 ccaccacgtg gtgctcatcc ggcagttccg gcatctcctc cgcggcgatt cgtgggagct
     1741 gcccgggggc gggggccagc ccggggagac cccggaggcg gccgcgcagc gcgaactgca
     1801 ggaggagggc ggctaccgcg cggggcgcct gaccttcctc acccgcttcg tcccctccgc
     1861 ggcctatctg gacgagaccg cctactgctt catcggcgag gagctggtgg ccgatcccct
     1921 cgcctccgac gacgacgagt tcttcgagcg gcgcgtggtc ccctttcgcg acgcggtggc
     1981 catggccctc gacgaccgca tcaccgagtc cgtctccaag gtcgccctcc tcgccgccgc
     2041 cctccgccgc ggcctccgcc cctagcgtcg ctccgcggtc acccgctgcg tcgaccgcgc
     2101 gcgcggttca tcgacgggcg gggccggggg cgggggcgcc tcccgggagc acgctgactc
     2161 gcgcggccgc tccggcactc aatcgagcca cgagaca
//
LOCUS       DASUFA010000255         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_161508, whole genome shotgun sequence.
ACCESSION   DASUFA010000255 DASUFA010000000
VERSION     DASUFA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161508"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..402)
                     /locus_tag="VFX87_09515"
     CDS             complement(43..402)
                     /locus_tag="VFX87_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4995196.1"
                     /translation="MADASCFHNLKEGGIPRQLAEGLSARVFPGEHLMLSVVEIQPGS
                     VSPVHSHANEQWGVCLEGEWIRIQDGVEHPVKAGDFWQTPPHVPHGGRATDKRAVVLD
                     IFSPPREEYKKAGSGYK"
     gene            447..1931
                     /locus_tag="VFX87_09520"
     CDS             447..1931
                     /locus_tag="VFX87_09520"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX4995197.1"
                     /translation="MRVFLAMLSHETNTFSNVPTDRVQFEARDLRYGGEIVETYRGTG
                     TCLGGMIAAAERLGITLLPSVAASASPAGLVTRDIYEHVKQRMLADLKAAGRLDGVLL
                     DLHGAMVPEGVDDGEGDIIAAVRRAVGPDVPIAVTLDFHGNLGKDMIAGADLLHGYKT
                     YPHVDMAERGVEAAERLAQVIGGRIKPTAAWRKPPLMPPLGRQGTARGPMRRLYDLAG
                     EMERDPKVISISLFAGFPYADIPDAGLGIYVVTDDDKALAERLADQLARVAWEHRHEF
                     IHTALPVKEAVAKALAAEGRPIVLADMADNTGGGAAGDGTEILRELLRVGARSATVAC
                     IWDAAAVQACVKAGVGAHVTLDVGGKVDDRHGAPMRVTGTVRTLSDGRFIHRGPMMKG
                     LPGRLGATAVLDVNDVKVILISYRWQTLDPEMIRFVGIDPLREKVLVVKSTIHYRAAF
                     EPIAKEIIEVDAPGLSSSNLARFEYRRVRRPIFPLDPDTVYEPE"
     gene            1946..2575
                     /locus_tag="VFX87_09525"
     CDS             1946..2575
                     /locus_tag="VFX87_09525"
                     /inference="COORDINATES: protein motif:HMM:NF018084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1054 family protein"
                     /protein_id="HEX4995198.1"
                     /translation="MSFTGFTPSDFRVFDIPGFKPRMEAIKARVRPKLEAVGRELLPD
                     VARIGGAPAFAHVAKHARRIVNPPEDTWVAFALDKRGYKKHCHFKVAVSRHGVRFLFE
                     AGPEHAQKKRWLAAWKRHTRQLVPVLRRAKGLGWFKDEHDEAPSAILTDLPADAVARL
                     GDELLRTRDGQFVLGRCVPAEEAARWKPREYARAARETFHLLAPLYRLK"
     gene            2734..>3053
                     /locus_tag="VFX87_09530"
     CDS             2734..>3053
                     /locus_tag="VFX87_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX4995199.1"
                     /translation="MIDSGAHHRHAWRALLAELGTEPAHPEHWRLTIGRPSEEAIPLL
                     LGRRMSGAEARRLARRKRDLYQERAQAGLEPVPGVPQFLEALARLGVPRAVGTSASRW
                     DAERL"
BASE COUNT          435 a         1086 c         1094 g          438 t
ORIGIN      
        1 gcgggggtgg ccggcccggg gtggcggggg cgcccgtccc gactacttgt agccggatcc
       61 ggccttcttg tactcctcgc gcggcggcga aaagatgtcg aggaccaccg cccgcttgtc
      121 ggtggcgcgg ccgccgtgcg gcacgtgggg cggcgtctgc cagaagtcgc ccgccttgac
      181 gggatgctcg acgccgtcct ggatgcggat ccactcgccc tcgaggcaca cgccccactg
      241 ctcgttggcg tgcgagtgca ccggggacac cgagccgggc tggatctcca ccaccgagag
      301 catgaggtgc tcgccgggaa acacgcgggc gctcaatccc tccgcgagct gccgcgggat
      361 gccgccctcc ttcaggttgt gaaagcacga cgcgtcggcc atgatttcct cctttcctct
      421 ggagcgccat tctacaggag acccgcgtga gagtgttcct ggccatgctg tcgcacgaga
      481 ccaatacctt cagcaatgtc cccaccgatc gcgtccagtt cgaggcgcgc gatctgcgct
      541 acggcggcga gatcgtcgag acctatcgcg gcaccggcac ctgcctgggc ggcatgatcg
      601 cggccgccga gcgcctgggg atcacgctgc tgccgtcggt tgcggcctcc gcctcgcccg
      661 ccggtctcgt cacccgcgac atctacgagc acgtcaagca gcgcatgctg gccgatctca
      721 aggcggcggg ccgtctcgac ggggtgctcc tcgacctgca cggagcgatg gtcccggagg
      781 gcgtggacga cggcgagggc gacatcatcg cggcggtgcg ccgggcggtg ggccccgacg
      841 tgccgatcgc ggtgacgctc gactttcacg gcaacctggg caaggacatg atcgcggggg
      901 ccgatctgct gcacggctac aagacctacc cgcacgtgga catggccgag cgcggcgtgg
      961 aggcggccga gcggctcgcc caggtgatcg gcgggcgcat caagccgacc gcggcctggc
     1021 gcaagccgcc gctgatgccg cccctcggcc gccagggcac cgcgcgcgga ccgatgcggc
     1081 gcctctacga tctggccggc gagatggagc gagacccgaa ggtgatctcg atctcgctct
     1141 tcgcgggctt cccctacgcg gacatccccg acgcgggcct cggcatctac gtggtgacgg
     1201 acgacgacaa ggctctcgcg gagcggctcg ccgaccagct cgcgcgcgtg gcgtgggagc
     1261 accggcacga gttcatccac accgcgctgc cggtgaagga ggcggtggcc aaggcgctcg
     1321 ccgccgaggg ccggccgatc gtgctcgccg atatggccga caacacgggc ggcggcgcgg
     1381 ccggcgacgg caccgagatc ctgcgcgagc tgctgcgggt gggcgcgcgg agcgcgacgg
     1441 tggcgtgcat ctgggacgcg gcggcggtgc aggcgtgcgt gaaggcgggg gtcggcgccc
     1501 acgtgaccct cgacgtgggc ggcaaggtgg acgaccgcca cggcgcgccc atgcgcgtga
     1561 ccggcacggt gcgcacgctg tccgacgggc gattcatcca tcgcgggccc atgatgaagg
     1621 gcctgccggg ccggctcggg gccaccgcgg tactagacgt gaacgacgta aaggtgatac
     1681 tcatctccta ccgctggcag accctcgacc ccgagatgat ccggttcgtc gggatcgacc
     1741 ccctcaggga gaaagtcctg gtcgtgaagt ccaccattca ctaccgcgcg gccttcgagc
     1801 cgatcgccaa ggagatcatc gaggtcgacg cgccggggct gtcctcgtcc aacctcgcgc
     1861 gcttcgagta tcggcgcgtc cggcgcccca tcttcccgct cgatcccgac accgtgtacg
     1921 agccggagta gaaggaggcc cccgcatgtc gttcaccggc ttcacgccgt ccgacttcag
     1981 ggtgttcgat atccccggct tcaagccccg catggaggcg atcaaggctc gcgtccgccc
     2041 caagctcgag gcggtgggcc gggagctgct gcccgacgtg gcccgcatcg gcggcgcccc
     2101 cgccttcgcg cacgtggcca agcacgcgcg gcgaatcgtg aacccgcccg aggacacctg
     2161 ggtggccttc gccctggaca agcgcggcta caagaagcac tgccacttca aggtcgcggt
     2221 ctcgcgccat ggggtgcgct tcctcttcga ggccggcccg gagcacgcgc agaagaagcg
     2281 ctggctcgcc gcctggaagc ggcacacgcg ccagctcgtc cccgtgctcc ggcgggccaa
     2341 gggtctgggc tggttcaagg acgagcacga cgaggccccg tccgccatcc tcaccgacct
     2401 gcccgccgac gcggtggcgc ggctcggcga cgagctcctg cgcacgcgcg acggccagtt
     2461 cgtcctcggc cgctgcgtgc ccgcggagga ggccgccagg tggaagccgc gcgagtacgc
     2521 gcgggcggcg cgggagacgt ttcacctgct ggcgccgctg taccgcctga agtgatcgcg
     2581 gtctctcgaa cgattgtccg ccgactgggg gagtgcgggg gccatttctg ggcccccgca
     2641 tttgatagag gggaggatcc gatcgtccgc ccccgcgggt cgtctcggga ctgatcggtg
     2701 accgctagcg cgatcttcga tatggatggg gtgttgatcg atagtggggc gcaccatcgg
     2761 cacgcgtggc gcgcgctgct cgccgagctg ggcaccgagc ccgcccatcc cgagcactgg
     2821 cgactgacca tcggccggcc cagcgaggag gccatcccgc tcctgctggg ccgccgcatg
     2881 agcggcgcgg aggcacgccg cctcgcccgg cgcaagcgcg acctctacca ggagcgcgcg
     2941 caggccgggc tcgagccggt gcccggggtg ccccagttcc tcgaggcgct ggcccggctc
     3001 ggcgtgccgc gggccgtcgg cacctccgcg tcgcgctggg acgccgagcg cct
//
LOCUS       DASUFA010000256         7100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1098333, whole genome shotgun sequence.
ACCESSION   DASUFA010000256 DASUFA010000000
VERSION     DASUFA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098333"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFX87_09535"
     CDS             complement(<1..938)
                     /locus_tag="VFX87_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995200.1"
                     /translation="MIRFSRSLMILGGLTLALSLAPVPAVAQEQPRSGGELVFVVAAE
                     PPGFDGHREETFAMLHPTGPHYNTLMRVDPTDRTGTKFIGDLAESWTVSPDKRVYTFK
                     IRRGVKFHDGSLMTSADVKASYDKIIFPGPGLVSSRKAAYRGVESVEAPNADTVVFRL
                     KAPEASFIANVSSPWNYIYKAEILAKDPHWYEKNIMGTGPFKFVEYVRGSHWVGKKNP
                     DYWDKGKPYLDGYRAIFIQDAGAQVAAIRGERAMIQFRGFTPAQRDGLVQALGNKITV
                     QESPWNCSIQVAMNQEKKPFQDKRVRRALSLALDRYA"
     gene            complement(1002..1949)
                     /locus_tag="VFX87_09540"
     CDS             complement(1002..1949)
                     /locus_tag="VFX87_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995201.1"
                     /translation="MATELGVLIPTREVIMSGRPETAPLLAMAERAEAAGFDSVWIGD
                     SITARPRHEPLTLMAAIAARTRRVRIGTGVLLPALRNPVVLAHLVGTVDRLAEGRVIL
                     GVGIAADSPAIRKEFAACGVPWDRRVGRFLETLEICRALWQRDGVTFTGKHFTLEGST
                     VEPKPHRPGGPPIWIGGGGPTAFREAARFDAWFPTGPSPEFFAEHFPRIRAAARAAGR
                     PADAVAGAAYVTLALDPDKGKAERRLHSFLETYYAAPAAAMMARQATYAGPIEGCVEW
                     LQRWIDAGARHLALRFAGGDQLAQVDEAATKLLPRLKTG"
     gene            complement(1952..5473)
                     /locus_tag="VFX87_09545"
     CDS             complement(1952..5473)
                     /locus_tag="VFX87_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4995202.1"
                     /translation="MICSGCGQANPAAAQFCGGCGARLAAVCPACRTENPPGNRFCHQ
                     CGAALAPKPEPAAPPDQFASPTAYTPKHLADKILTTAGALKGERKQVTVLFVDVSGFT
                     ALSAGLDPEEVHRLMSRAFDLMLTEVHRFEGTVNQFLGDGIMALFGAPIAHEDHARRA
                     VHAALGIARALDAYQTELRSRGITFRVRQGLNTGLVVVGSIGSDLRMDYTAVGDTTNV
                     AARMQQVGEPGRVTISEATHRLVHGYFETRALGEKQLKGKAEPMPAWEVVAARETRTR
                     LDVEAEHGLTPLVGRERELGQLLDAFARARSGEGQVVFVVGEPGIGKSRLLLELRRRV
                     GASGDWHEGHCLSFGKAMAYHPLVDLIRRWFGVEESDGETAIAAKLERGVAEIDADLG
                     PVTPYLRALLSIDPGDDGVRDMSPEERRGGILEALRRLLDRAAERQAQILVIEDLHWI
                     DSASEQFLAMLVDSVPAARAVLVFTYRPGYPNPFGDRSYFTRIVPAPLSADDSVRMAA
                     AVLATDTLPAELRALVATKAEGNPFYVEELVKSLEEGGVLRRANGRLDLARPVTASDI
                     PSTIQDVIAARIDRLAEAPKQTLQLASVIGREFTRRLVDRLAEIRERTEDYLRELTAL
                     ELIHERRRFPELAYMFKHALTQDVTYASLLVRRRKELHGLTGRAIEDLYADRLPEHFE
                     MLAHHFSRAEDWDRALDYLLKAARKATQAFGLRQALDLYAEALAVAARRVEPVPPATI
                     MEIQRARADLFFGVGEFVRSREAAEALVDLARRVGDRAAEAGALVQIALALQWAEDFP
                     PAFERVREAIEIAENVGAQAPLSFGLYTQGYLHALNGRLDESEADVGRALTIGRAVGD
                     PNRQALALHFLSLCRSWQGDYRPSLDLSGEGIRIAREEHLVVPLLRCLWNEGLAASDL
                     GEYDRALAALEEGLTLAEKIGDDAYISRCLNTIGWLRIECGDSARGVELSEQSYEVTG
                     RSSRAGHGTGAERRAFIRNNQAEAWMARGDLAAAAEALDEAHHIVRHPPASRWMTWRY
                     STQCYAGLGQLALLQGDPERARRLADQSLEICVPTRSRKFESWAWRIKGESATARHAW
                     DEADEALRRALTIAEAIGQPRQTWVSQVALGRLDAARGRREEALGRYRTAWAIITRLR
                     AATRDPGLRAGLESSPLIHEVESLIGR"
     gene            complement(5490..6458)
                     /locus_tag="VFX87_09550"
     CDS             complement(5490..6458)
                     /locus_tag="VFX87_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4995203.1"
                     /translation="MLMRKLGRTGLKVSALCLGGNTFGWTTDQKASEAVLDAYVEAGG
                     NFIDTADVYSRWAPGNKGGESETALGTWLTDRKNRASVLIATKVCGPMGPGPNDAGLS
                     RAHIMEGVEASLRRLQTDYIDLYQAHWDDKETPLEETLRAFDDLVRQGKARYIGASNY
                     PAWRLTRALWESERHGYVRYECLQPKYNLVTRDEYERELEPLCLEQTIGVIPYFSLAS
                     GFLSGKYRSGEPMPKTARAAGVEKTYMNERGFRVLAAVEKVAAAANATPAQVSLSWLA
                     HRPGITAPIASATSAAQVKELAGGIELKLDAGATAALDQAGAWRES"
     gene            complement(6469..>7100)
                     /locus_tag="VFX87_09555"
     CDS             complement(6469..>7100)
                     /locus_tag="VFX87_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HEX4995204.1"
                     /translation="EAYLARLVDVMKEEYDAIHRAGFVLQVDCPDLAMSRHLAFPDLS
                     NAAFVKLAEGNVEALNHALRDIPPDRLRLHLCWGNYEGPHHRDIPLREILAVVLKARP
                     QALSFEGANPRHAHEWVVFRETKLPDDKVIIPGVLDTTTNFIEHPELVAQRLVRYADV
                     VGRERVIAGSDCGFATFARSQPLVEPEIVWAKLKSMAEGARLASGQLWK"
BASE COUNT          993 a         2515 c         2526 g         1066 t
ORIGIN      
        1 gcgtaccggt ccagcgccag cgagagcgcc cggcgcacgc gcttgtcctg gaacggcttc
       61 ttctcctggt tcatcgccac ctggatcgag cagttccagg ggctctcctg caccgtgatc
      121 ttgttgccga gcgcctggac cagaccatcc cgctgcgccg gggtgaagcc gcggaactgg
      181 atcatcgccc gctcgccgcg aatggccgcc acctgggcgc ccgcgtcctg gatgaagatg
      241 gcgcggtagc cgtcgaggta gggcttgccc ttgtcccagt agtcggggtt cttcttgccc
      301 acccagtgcg agccccgcac gtactcgacg aacttgaagg gcccggtgcc catgatgttc
      361 ttctcgtacc agtgcgggtc cttggccagg atctcggcct tgtagatgta gttccacggc
      421 gaggacacgt tggcgatgaa cgaggcctcc ggggccttca gccggaacac caccgtgtcc
      481 gcgttcgggg cctccaccga ttccacgccc cggtaggccg ccttccggct cgacacgagc
      541 cccggccccg ggaagatgat cttgtcgtag gaggccttca cgtcggcgga ggtcatcagg
      601 ctgccgtcgt ggaacttcac cccgcggcgg atcttgaagg tatagacgcg cttgtccggc
      661 gagaccgtcc aggactcggc gaggtcgccg atgaacttgg tgccggtccg gtcggtggga
      721 tccacccgca tcagggtgtt gtagtggggc ccggtcgggt ggagcatggc gaacgtttct
      781 tcgcgatgcc catcgaagcc cggcggctcg gccgcgacca cgaagaccag ctcaccgccg
      841 gagcggggct gctcctgggc gacggccggc acgggggcga gcgagagagc aagggtgaga
      901 ccgccgagga tcatcagtga tcgcgagaac cggatcatgt ccacctccga tggggtatgg
      961 gtcgagcgcg agtctaccag aagcggcccg tgatctgagg tctagcccgt cttgagccgc
     1021 ggcaacagct tcgtcgccgc ttcgtccacc tgcgcgagct ggtcaccgcc cgcgaagcgc
     1081 agggccaggt ggcgggcgcc cgcgtcgatc cagcgctgca gccattccac gcagccctcg
     1141 atgggcccgg cgtaggtggc ctggcgcgcc atcatcgcgg cggccggcgc ggcgtagtac
     1201 gtctcgagga acgagtgcag ccgccgctcg gccttgccct tgtcgggatc cagcgcgagc
     1261 gtcacgtagg ccgcgccggc caccgcgtcg gccggacggc ccgcggcgcg ggcggccgcc
     1321 cggatgcgcg ggaagtgctc ggcgaagaac tccgggctcg ggccggtcgg gaaccacgcg
     1381 tcgaagcgcg cggcctcgcg gaaggcagtc gggccaccgc cgccgatcca gatgggcggc
     1441 ccgccgggcc ggtgcggctt gggctcgacg gtggagccct ccagcgtgaa gtgcttgccg
     1501 gtgaacgtga cgccgtcgcg ctgccagagc gcgcggcaga tctcgagcgt ctcgaggaag
     1561 cggcccaccc gccggtccca cgggacgccg caggccgcaa actctttccg gatggccggg
     1621 ctgtccgcgg cgatgcccac gcccaggatc acccgcccct cggccagtcg gtccaccgtg
     1681 ccgaccaggt gcgcgagcac caccgggttg cgcagggcgg gtagcagcac cccggtgccg
     1741 atgcgtacgc gccgggtgcg cgccgcgatc gccgccatca gggtcagcgg ctcgtgacga
     1801 ggccgcgcgg tgatcgagtc cccgatccac accgaatcga agcccgcggc ctcggcccgc
     1861 tcggccatcg cgagcagcgg cgccgtctcg ggccggcccg acatgatgac ctcgcgggtg
     1921 ggaatgagca caccgagctc ggttgccatc gctacctccc gatcagggat tcgacttcat
     1981 ggatgagcgg cgacgactcg agcccggccc gcaggccggg gtcccgggtt gccgcccgta
     2041 gccgggtgat gatagcccag gccgtgcggt agcgcccgag cgcctcctcc cgtcgccccc
     2101 gcgccgcatc gagccggccg agtgccacct ggctcaccca ggtctggcgc ggctggccga
     2161 tcgcctccgc gatggtcagt gcgcgccgca gcgcctcgtc cgcctcgtcc caggcatggc
     2221 gcgcggtcgc gctctctccc ttgatacgcc acgcccagct ctcgaacttc cgcgaccggg
     2281 tcggcacgca gatctcgagg ctctgatcgg cgagtcgccg ggcccgctcg ggatcgccct
     2341 ggaggagggc gagctgaccc aggcccgcgt agcactgcgt cgagtagcgc caggtcatcc
     2401 agcgcgaggc cggcggatga cggacgatgt gatgcgcctc gtccagcgcc tcggcggccg
     2461 cggcgagatc cccgcgggcc atccaggcct cggcctggtt gttgcggatg aacgcgcggc
     2521 gctcggcccc ggtgccgtgg ccggcccgag aggagcgccc ggtgacctcg tacgattgct
     2581 ccgatagctc gacgccccgg gcggagtcgc cgcactcgat gcgcagccag ccgatcgtgt
     2641 tgaggcatcg cgagatgtag gcgtcgtcgc cgatcttctc cgcgagagtg aggccctcct
     2701 cgagcgcggc gagggcccgg tcgtactctc ccaggtcgct ggcggccagg ccctcgttcc
     2761 acaggcagcg gagcagcggg acgacgagat gctcctcgcg cgcgatccgg attccctccc
     2821 cgctgagatc gaggctcggc cggtagtccc cctgccagct ccggcacagc gagaggaaat
     2881 gcagcgccag cgcctggcga ttcggatcac ccaccgcccg gccgatggtc agggcccgtc
     2941 cgacgtcggc ctccgactca tcgagacgcc cgttgagcgc gtgcagatag ccctgggtgt
     3001 acagcccgaa gctcagcggg gcctgggccc ccacgttctc ggcgatctcg atcgcctctc
     3061 gcacccgctc gaacgcgggc gggaagtcct cggcccactg caaggcgagg gcgatctgca
     3121 ccagagctcc cgcctccgcc gcgcgatctc cgacccgacg ggcgaggtcc acgagggcct
     3181 cggccgcctc ccgggagcgg acgaactcgc cgacaccgaa gaacaggtcg gcgcgggcgc
     3241 gctggatctc catgatcgtc gccgggggta ccggctcgac ccgccgggcc gccaccgcga
     3301 gcgcctcggc atacagatcc agggcctgcc gcaggccgaa cgcctgggtc gccttccggg
     3361 cggccttgag caggtagtcg agagcgcggt cccagtcctc ggcgcgcgag aagtgatgcg
     3421 cgagcatctc gaagtgctcg gggagccgat ccgcgtacag atcctcgatg gcccgcccgg
     3481 tgagtccgtg cagctccttg cgccgccgca cgaggaggga ggcgtaggtc acgtcctggg
     3541 tcaaggcgtg cttgaacatg tacgccagct cggggaagcg gcggcgctcg tggatcagct
     3601 cgagcgcggt cagctcacgg aggtagtcct cggtcctctc ccggatctcg gcgagacgat
     3661 ccacgagccg ccgggtgaac tcgcgcccga tcaccgacgc gagctgcagc gtctgcttgg
     3721 gcgcttcggc cagacggtcg atccgggccg cgatcacgtc ctggatggtg ctgggaatgt
     3781 cggacgcggt caccggccgg gccaggtcga gccggccgtt cgcccgccgc aggactcctc
     3841 cttcctccag tgacttgacc agctcctcga cgtagaacgg gttgccctcg gccttggtgg
     3901 cgacgagcgc gcgcagctcg gcgggcaggg tgtcggtggc gaggaccgcc gccgccatgc
     3961 ggacgctgtc gtcggccgac aacggcgccg ggacgatccg ggtgaagtag ctgcgatcgc
     4021 cgaagggatt cgggtatccc ggccgatacg tgaagacgag caccgcccgg gccgccggca
     4081 cgctgtccac cagcatcgcg aggaactgct cgctcgcgct gtcgatccag tgcaggtcct
     4141 cgatgaccag gatctgcgct tgccgctcgg ccgcgcggtc gaggaggcgg cggagcgcct
     4201 cgagtattcc gccgcggcgc tcctcggggc tcatgtcccg cacgccgtcg tcgcccgggt
     4261 cgatggagag caaggcccgc aggtacggcg tgacgggccc gagatcggcg tcgatctccg
     4321 cgactccccg ctcgagcttc gccgcgatgg cggtctcgcc atcgctctcc tcgacgccga
     4381 accagcgccg gatcaggtcg accagcgggt gatacgccat ggccttgccg aacgagaggc
     4441 agtggccctc gtgccagtcg ccgctggcgc cgacccggcg gcgcagctcg aggagcaggc
     4501 gcgacttccc gatgcccggc tcgccgacga cgaataccac ctgcccctcg ccggaccggg
     4561 cacgggcgaa ggcatcgagc agctggccca gctcgcgctc gcgcccgacc agcggggtga
     4621 gcccgtgctc cgcctcgacg tcgaggcgcg tgcgcgtctc gcgcgcggcc acgacttccc
     4681 aggcgggcat cggctccgcc ttgcccttca gctgcttctc gcccagcgcg cgggtctcga
     4741 agtagccgtg cacgagccga tgggttgcct ccgagatcgt cacccgcccg ggctcgccca
     4801 cctgctgcat gcgcgccgcc acgttggtgg tatcgccgac cgcggtgtag tccatgcgca
     4861 ggtcgctgcc gatgctgccc accaccacga ggccggtatt gaggccctgg cggacgcgaa
     4921 aggtgatgcc gcgtgaccgc agctcggtct ggtaggcgtc gagcgcgcgc gcgatgccga
     4981 gggcggcatg gacggcgcgg cgggcgtggt cctcgtgcgc gatcggcgcc ccgaagagcg
     5041 ccatgatgcc gtcgccgagg aactggttga ccgtgccctc gaagcggtgc acctcggtca
     5101 gcatcagatc gaacgcgcgg ctcatgagcc ggtggacttc ctcgggatcg aggccggcgg
     5161 agagggccgt gaagcccgag acgtccacga agaggaccgt cacctgcttg cgctcgccct
     5221 tgagcgcgcc cgcggtggtg aggatcttgt cggccaggtg cttcggggtg taggcggtcg
     5281 gcgaggcgaa ctgatcggga ggagcggccg gctcgggctt cggcgcgagg gcggcgccac
     5341 actggtggca gaagcggttg cccggcgggt tctccgtccg gcacgcggga cagaccgcgg
     5401 ccaggcgcgc gccgcatccg ccgcagaact gggcggcggc cggattcgcc tggccgcagc
     5461 cagagcagat catagacccg gcgttgggcc tagctctcgc gccacgcgcc ggcctggtcg
     5521 agcgcggccg tggcccccgc gtccagcttg agctcgatcc cgcccgccag ctccttgacc
     5581 tgcgccgccg acgtggcgct cgcgatcggc gcggtgatcc ccggccggtg cgccagccac
     5641 gagagcgaca cctgcgcggg cgtggcgttg gcggccgccg ccaccttctc gaccgcggcc
     5701 agcacgcgga agccgcgctc gttcatgtag gtcttctcca cgccggcggc ccgggcggtc
     5761 ttgggcatcg gctcgcccga acggtacttg cccgacagga acccgctggc cagcgagaag
     5821 tacgggatca cgccgatcgt ctgctccagg cagagaggct cgagctcgcg ctcgtactcg
     5881 tcgcgggtca ccaggttgta cttgggctgc aggcactcgt agcgcacgta gccgtgccgc
     5941 tcgctctccc agagcgcacg ggtgagccgc caggcgggat agttcgaggc cccgatgtag
     6001 cgcgccttgc cctggcgcac caggtcgtcg aaggcgcgca gcgtctcctc gagaggggtc
     6061 tccttgtcgt cccagtgcgc ctggtagagg tcgatgtagt cagtctgcag gcgccggagc
     6121 gaggcctcca ctccctccat gatgtgcgcg cgcgagaggc cggcgtcgtt tggacccggc
     6181 cccatcggcc cgcacacttt ggtggcgatc agcacgctcg cgcggttctt gcggtcggtc
     6241 agccaggtgc cgagagcggt ctcggactct ccgcccttgt tgccgggcgc ccagcgggag
     6301 tagacgtcgg cggtgtcgat gaagttgccg cccgcctcga cgtaggcgtc cagcaccgcc
     6361 tcggaggcct tctggtcggt ggtccagccg aacgtgttgc cgcccaggca cagcgcggac
     6421 accttgagcc cggttcgccc cagcttgcgc atcagcatga tgagtctcct atttccagag
     6481 ttggccggac gccagccgcg cgccctcggc catggatttg agcttggccc agacgatctc
     6541 cggctcgacc agtggctgcg agcgcgcgaa ggtggcgaag ccgcagtcgc tgcccgcgat
     6601 cacccgctcg cgccccacca cgtccgcgta gcggacgagg cgctgcgcga ccagctcggg
     6661 gtgctcgatg aagttggtcg tggtgtcgag gacgcccggg atgatcacct tgtcgtcggg
     6721 cagcttcgtc tcccggaaca cgacccattc gtgggcgtgg cgcgggttgg cgccctcgaa
     6781 ggagagcgcc tgcgggcgcg ccttcaggac caccgccagg atctcgcgca acgggatgtc
     6841 gcggtggtgc ggcccctcgt agttgcccca gcagaggtgc aggcgcaggc gatcgggcgg
     6901 gatgtcgcgg agcgcgtggt tgagcgcctc gacgttgccc tcggcgagct tcacgaacgc
     6961 ggcgttgctc aggtcgggaa acgccaggtg ccggctcatg gccaggtccg ggcaatccac
     7021 ctggagcacg aagccggcgc ggtggatggc gtcgtactcc tccttcatca cgtccaccag
     7081 ccgggccagg taggcctcgc
//
LOCUS       DASUFA010000257         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_66874, whole genome shotgun sequence.
ACCESSION   DASUFA010000257 DASUFA010000000
VERSION     DASUFA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66874"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VFX87_09560"
     CDS             <1..1235
                     /locus_tag="VFX87_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX4995205.1"
                     /translation="VVVVEHDRSFIQAADYVVEMGPGSGDRGGTVIFAGNQAEFARDP
                     HSLTARYLTGRDGIPLPITRREGRRALVVRGARAHNLKDVTLRIPLHTLTCVSGVSGS
                     GKSTLVHDTLYRAVARHFKVDFKTPGEHDEVRGLEYLKGVRLIDQEPIGRTPRSNPVT
                     YVKVFDEIRKLFAALPRARTLGLNSGAFSFNVPGGRCEACEGGGFQKLEMYFFEDVYV
                     SCPECEGRRYRSEVLKVTYKGKDISQVLQLTVDEAVDFFGTHTVLARRLKLLQDVGLG
                     YLRLGQPATTLSGGEAQRLKIAAELGTRQTTDHLYILDEPTTGLHLDDVKKLLVVLSR
                     LVDTGNTVLVVEHHLDVIKTADWVVDLGPEGGGAGGAIIAEGTPEQLAQVDGSYTGKF
                     LRELLPRPNGRGAGGRVE"
     gene            1232..2518
                     /locus_tag="VFX87_09565"
     CDS             1232..2518
                     /locus_tag="VFX87_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HEX4995206.1"
                     /translation="MTAHHSLEAVGILVLGLLFYSYTIGWVPGPRPADRLRRTLLHGL
                     GFGALTVATMIARIEIAPGIYIDPRAVPVALIGLFEGWPPALLAAAMGCAYRLLIGGD
                     GMWAGVFSLLGTAAVAGLVHVWVRHDGGLRPRHVIGLTAASYVVTFLGFSMLGAWGLA
                     LFSRVWTGYAITFAIGIGLVARLFYDVAEQHRLTGEQGRFRAVLDEAADAIRIIDCDS
                     QRIVDVNRADCSLSGYTREELVGRLRRDFWPEGPEFRLAQEASLAETYAAGFLETRGS
                     PFRTRGGAVVPVDTTQRIVEFEGRRYEIVMWHEAGPRIAAETADRETAELRAATLLAR
                     AAAHEINNPLAVVLGYLQLMGSRMAPETKEAGWIRQMTDACGRIRDAVGRFNSITKIE
                     ATTPIGGAPPMLDTARSSVPAAAVPEPPTPSRPPPP"
     gene            2515..>2981
                     /locus_tag="VFX87_09570"
     CDS             2515..>2981
                     /locus_tag="VFX87_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995207.1"
                     /translation="MRGPLYYGWVVAAGAFLVLFLAYGTQYAFGVFFAALLAEFHWSR
                     ASLSGAFSLYAFVYSAFGLVAGRLTDRWGPRAVIGGGGVLLGAGLAGMSRVDALWQPY
                     VLYGGVAALGMSTAFVPCNATVVRWFVRRRGLAVGLASAGGSLGTFVLPPVAH"
BASE COUNT          389 a         1108 c         1001 g          483 t
ORIGIN      
        1 ccgtggtggt ggtcgagcac gaccgctcgt tcatccaggc ggcggactac gtggtggaga
       61 tgggccccgg ctcgggcgat cgcggcggca ccgtcatctt tgcggggaac caggcggagt
      121 tcgcgcgcga cccgcactcc ctgaccgcgc gctatctcac cggccgcgac ggcatcccgc
      181 tgcccatcac ccggcgcgag ggccggcgcg ccctcgtggt gcggggcgcc cgggcccaca
      241 acctcaagga cgtcaccctc cgcatcccgc tccacaccct cacctgcgtg tccggggtct
      301 ccggatccgg caagtccacc ctggtccatg acacgctcta tcgtgccgtc gcccgccact
      361 tcaaggtgga cttcaagacg cccggcgagc acgacgaggt gcggggtctc gaatacctca
      421 agggcgtacg cctcatcgac caggagccca tcggccgcac gccgcgctcc aacccggtca
      481 cctacgtgaa ggtcttcgac gagatccgca agctcttcgc ggcgctgccg cgggcgcgca
      541 ccctgggtct caactccggc gctttttcct tcaacgtgcc gggcggccgc tgcgaggcct
      601 gcgagggcgg cggcttccag aagctcgaga tgtacttctt cgaggacgtc tacgtgagct
      661 gcccggagtg cgaaggccgc cgctaccggt ccgaggtgct caaggtcacc tacaagggga
      721 aggacatcag ccaggtcctg cagctcaccg tggacgaggc ggtggacttc ttcgggaccc
      781 ataccgtcct ggcgcggcgc ctcaagctgc tgcaggacgt gggcctgggc tatctccggc
      841 tgggccagcc cgccaccacg ctgtccggcg gcgaggcgca gcggctgaag atcgcggccg
      901 agctggggac gcgccagacc acggaccacc tgtacatcct cgacgagccg accaccgggc
      961 tgcatctcga cgacgtcaag aagctgctcg tcgtgctcag ccgcctggtc gacaccggca
     1021 ataccgtcct cgtggtcgag catcacctcg acgtcatcaa gaccgcggac tgggtggtgg
     1081 acctcgggcc cgagggcggc ggggccggcg gcgccatcat cgccgagggc acgccggagc
     1141 agctggccca ggtggacggc tcttataccg gcaagttcct tcgcgagctg ctgcctcgac
     1201 ccaatggcag gggcgccggc ggtcgagtgg aatgaccgcc caccacagcc tcgaagcggt
     1261 cgggatcctc gtcctgggcc tgctcttcta ctcgtacacg atcggctggg tccccggccc
     1321 gcgcccggcc gaccgactcc gtcgcaccct gctccacggc ctgggcttcg gcgcgctcac
     1381 ggtggccacg atgatcgccc gcatcgagat cgctcccggc atctacatcg atccccgcgc
     1441 ggtgccggtg gccctcatcg gcctcttcga aggctggccg cccgccctgc tggccgccgc
     1501 gatgggttgc gcctaccgac tcctgatcgg cggcgacggc atgtgggccg gagtgttcag
     1561 cctcctcggc accgccgcgg tcgcgggact cgtgcacgtt tgggtacggc acgacggcgg
     1621 gttgcggccc cgacacgtca tcggcctcac cgccgcctcc tatgtggtca ccttcctcgg
     1681 attctcgatg ctcggcgcgt ggggcctggc tctcttctca cgggtgtgga cgggctacgc
     1741 catcaccttc gccatcggca tcgggctggt ggcgcggctc ttctacgacg tggccgagca
     1801 gcatcggctg accggcgagc agggccgctt ccgcgccgtg ctcgacgagg ccgccgacgc
     1861 catccgcatc atcgactgcg acagccagcg catcgtggat gtcaaccggg ccgactgctc
     1921 cctgtcgggc tatacccgtg aggagctggt gggacgcctg cggcgcgact tctggcccga
     1981 gggtcccgag ttccgcctgg cccaggaggc gtccctcgcg gagacgtacg ccgccgggtt
     2041 cctggagacg aggggctccc cctttcgcac gcgcggcgga gccgtcgtcc cggtggacac
     2101 cacgcagcgc atcgtcgagt tcgagggccg gcgctacgag atcgtcatgt ggcacgaggc
     2161 aggtccgcgc atcgcggcgg aaaccgcgga ccgggagacc gccgagctcc gcgccgccac
     2221 cctcctcgcg cgggccgccg ctcacgagat caacaatccg ctcgccgtcg tcctgggata
     2281 tctgcagctc atgggctccc gcatggcccc cgaaaccaag gaggcggggt ggatacgcca
     2341 gatgacggac gcctgcggcc gcatccgcga cgccgttggc cggttcaaca gcatcaccaa
     2401 gatcgaggcg accacgccca tcggcggcgc cccgcccatg ctcgacaccg cccgctcttc
     2461 cgtcccggcg gcggccgtcc ctgagccgcc cacgccgtct cgtccgcccc ctccgtgagg
     2521 ggcccgctct actacggctg ggtcgtcgcc gccggcgcct tcctggtcct cttcctcgcc
     2581 tatggaaccc agtacgcgtt cggcgtcttc ttcgccgcgc tcctggccga gttccactgg
     2641 tcgcgcgcca gcctgtccgg cgccttctcg ctctatgcgt tcgtgtacag cgcgttcggg
     2701 ctggtggcgg ggcgcctgac ggaccgctgg gggcctcgcg cggtgatcgg cgggggcggt
     2761 gtgctcctcg gagccgggct cgcgggcatg agccgcgtgg atgccttgtg gcagccctat
     2821 gtcctctacg gcggggtggc cgcgctcggc atgtccaccg ccttcgtccc ctgcaatgcc
     2881 accgtggtgc ggtggttcgt gcgccggcgc ggcctcgcgg tggggctggc cagcgcgggc
     2941 ggcagtctcg gcaccttcgt cctgcctccg gtcgcccact g
//
LOCUS       DASUFA010000258         9459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_984475, whole genome shotgun sequence.
ACCESSION   DASUFA010000258 DASUFA010000000
VERSION     DASUFA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984475"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..1335
                     /locus_tag="VFX87_09575"
     CDS             556..1335
                     /locus_tag="VFX87_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX4995208.1"
                     /translation="MAREVAGIVRVMQILESVAISPDGISVRDLSRAIGCGKGTISKM
                     LGTLERRHYVRRDAASGHFFLTWRLLALAFSHSDQAGMPRVFLPVLQELADETDELVQ
                     LAVVDGDQVLFVAKAEGRGQTIRLLPLVGLWAPLHATAAGKLWLSTLPASEVSRVLGR
                     RLPAVAPRTITSKGALRKELERVQAAGYALADEELAEGGRAIAAPIKREGAMVGAVAL
                     SGPAYRLSLERLHTLAPRVIEAAARLASIWPAHVNAKDFTS"
     gene            1332..2216
                     /locus_tag="VFX87_09580"
     CDS             1332..2216
                     /locus_tag="VFX87_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX4995209.1"
                     /translation="MSRFEYHQPESLHDAVALAARFGAEASFLAGGTDLIVQIGRGRI
                     APRHVVGLHRVPGLVGIETNGRISLGACVTHRSVERAPALAGALRCLIEGAEVIGGHQ
                     VRNVATVGGNLVNASPAADLVPCLLALDGAVTLLGPGGERDLPIERFLLGPNRTARQP
                     DELLTRVSLPAPPARAGTAFLKAGRRRAMEISVVCVAARLTLDASLERCLEARVALGA
                     VAPTAVRAHEAERALEGQPVGGEVFRRAAEAAQEACRPIDDVRASAAFRRHLVGVLVR
                     RALDRCVERARSHLDQTA"
     gene            2233..2745
                     /locus_tag="VFX87_09585"
     CDS             2233..2745
                     /locus_tag="VFX87_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4995210.1"
                     /translation="MARIPITLEINGERHEGLVEPDRTLLDVLRSDLGLTGTKTNCLD
                     AECGVCTVLVDGLAVNSCLYLAVVAQGRAITTIEGLAPAREGPTPAGTLHPLQQAFVE
                     HGAVQCGYCIPGMILAAKAYLDDHLQPSETQVREALAGNLCRCTGYQKIVDAVLAASS
                     RTAAAGTVVS"
     gene            2742..5015
                     /locus_tag="VFX87_09590"
     CDS             2742..5015
                     /locus_tag="VFX87_09590"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4995211.1"
                     /translation="MSGATRVVGHSLPRIDAPGKVTGSAIYAADFALPGMLYGKVFRS
                     AEPHARLVRLDTSRAADIAGVRAVISAADVPDVRYGAAVKDEPVFASDIVRYVGQPVA
                     AVAAVTLEAADAALATIEAVYEPLPRVLDLAAALAPGAPLVHEGWQAYTAIPILQRDG
                     NVCNRARIVVGDVERGWAEADRVFEHRFTTAMVHQGYTEPRAAVAAWDSSGQVTVWSN
                     TQLPFDVQATLADVLAIAPSKIRVIVPGVGGGFGGKLRVGVEHFAALLARKAGRPVKV
                     MTTSEEELTAAYPRQGTIIELKTGVTRDGRLTARQGRIYFDTGAFAGSGPGVASVATL
                     VLAGPYRTPNLHLEGYAVYTNKTNCGSYRAPSGPQANFAVESQMDIIADALGIDPLEL
                     RLRNIVPEGDEGPTGQVLSAVGLEECLRRAADAIGWRDRRPGPGRGKGLAIGWWTTTG
                     GSSGVYVKINPDGTVALNTGAVEIGTAALTGAAQVLAEVLGVDLADITVVSGDTFSTP
                     FDFGAQGSRTAFAVGNACRAAAAELQRKLFALAAAQLGAPEEALVLRDKHVVGPGGRM
                     SLAELARQSQVAGGGMIAHGTFIAPATAYDTKRVEGHVYPAFHSPSFHAHAVDLSVDA
                     ETGEVTIHKYVVAQDVGFALNPTYIEGQIEGGVAQGLGQTLSEEIVYRDGRVQNANLT
                     DYKIPTTLDVPRVESILVEHPGRVGPFGAKGVGEPPNVEPPAAVANAIAAAVGARITS
                     LPITAEKIVLGSHGGAR"
     gene            5015..6049
                     /locus_tag="VFX87_09595"
     CDS             5015..6049
                     /locus_tag="VFX87_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995212.1"
                     /translation="MPLTTGLLMLPSHPAGRLVEIAQLAERTGYDYLWLADERFFREV
                     YASLTLCALRTQRIKLGPCVTDPYSRHAALTAMAIATLDEISGQRAVLGIGAGVSGFR
                     ELGIARDKPGSGLREAVEVIRKLLAGETVTYKGAVVQIEGGRLDFKPMRADVPMYIAS
                     QRPVGVRAAGRVADGAIMQGCVAEPLLAFFRDTVAAGAREAGRDPARIDLVARINVCV
                     HDDRQVARDLMKPTIVRSLAAQRPDFFTFKTAGVTVPPALARHLEGLSYAYDPRPFQA
                     AAAEVPESFVDAVTLAGPPDEVAAGVARMARQGVTQFMLYPLSPDGRVELVIERFQSE
                     VMPRVRAAVR"
     gene            6046..6327
                     /locus_tag="VFX87_09600"
     CDS             6046..6327
                     /locus_tag="VFX87_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX4995213.1"
                     /translation="MTVTVVMHGTLRRFLPEGAARATLDLRAGATIGELLVELGADAD
                     TWLVARNQAVAERDAVLAPGDVLDCFEPLAGGAGAGDGGREASPCRYGT"
     gene            6309..6632
                     /locus_tag="VFX87_09605"
     CDS             6309..6632
                     /locus_tag="VFX87_09605"
                     /inference="COORDINATES: protein motif:HMM:NF023070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995214.1"
                     /translation="MPVRYVITTLKPGVKPEDYERWLVEYDYKVAKSLPSIVSYRTHR
                     IEGPINGAETAGWSYIERIEIRDVDQYQRDIASPAGQELMRQLYGNYLERSKNIFFWS
                     EEITD"
     gene            6645..8270
                     /locus_tag="VFX87_09610"
     CDS             6645..8270
                     /locus_tag="VFX87_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995215.1"
                     /translation="MGARGAGVSRRDFLVTAGVGAAGLAVGRRAAAQDKKTLVVAWDS
                     DIDSLDPAVFKSQGAYVTVANTTDAPIMWKVRPLDGKPGLFRSMPAEWDGHLAESWAM
                     EDNGATIVLKVRRGMKFPSGRPITAHAFKYSFDRGLLSPGYMKLIFPTLIQVSAPEQF
                     VVRDDYTFVINMKAPSPMGLDTVALSNNAILDPDVVKANGTKDDPWATEWLKRNGASV
                     GPYRLVKNEPGVEIVMEATPGYWRPKPYFDRVILKLVPNEADRVLLLKRKAVDLVAGR
                     PGLSPKNVKALEGEAGLKVFSVPDTTCHYLCMNQKKPPFDNKLVRQAVNYAIPIQAIL
                     PNVLFGYGTQMKSPVPNLTPSHLGDYSPYKHDIAKAKALMKEAGMDKAPIPVDLAIRV
                     GWPTHEQASVWLQRELEQIGFKVNIVKETDATFRQLATKGDHHLSIEAWQSWINDPFY
                     HLTFLFNSKSKFTNLSFYANPAVDKLIDDNMHETNRDKRAAASRDIQKLVLDDAVWGL
                     LWYENWTRVGTSDLVGLEKRWDTFERYYNLKRA"
     gene            8347..9204
                     /locus_tag="VFX87_09615"
     CDS             8347..9204
                     /locus_tag="VFX87_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995216.1"
                     /translation="MRIRVNGVELFVDVHGPALAWRDGATVEKPVLLALHGGPGLDHA
                     NLVPWLQPLAERAQILYVDHRGNGRSGRPPLEECTLEAMADDLEALRRLLGLGPLIVL
                     GVSFGGMVALTYALRHPAGLARLIACVTAASGDFVADARRLARARATPRQMEVVEHML
                     DGTFRDEAHFREGFAAVASLYEHRSDPARDAAALPSVLNVAMLNWFFATEVKRYDLRP
                     RLGEVRAPTLVLTGRHDWICAPAHSDTLVRGIPGARQVMFEESGHRVIKEENARFVRV
                     VTEFIHETT"
     gene            9218..>9459
                     /locus_tag="VFX87_09620"
     CDS             9218..>9459
                     /locus_tag="VFX87_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995217.1"
                     /translation="MIEAPKGYEPDFPPVFQGGSPEDHAALLRLYFDLRLANDGLDND
                     ALRKIWEPDAGHIYFNTNGHAYHGLEDWLHIWDHYRT"
BASE COUNT         1404 a         3282 c         3403 g         1370 t
ORIGIN      
        1 acctcgcgga gcagctcatc gcctcgcggg gtcgggccac cggcgcatga gttcggccat
       61 actgcaccgc gcgccctggt aggacctcag tcgtgtggcg tgcggcctga cgcctctcga
      121 ggcgacacct gaccggcgga cagttgctct ccaccccccg tagaccagcg tcggcgctgg
      181 cagtcggaac ggcgcggacg gcccctcgtg ctggcccgtg tgctgatgag cgcctcccgt
      241 ctcgtagaca gtcgtcgtct ggatgtccgc gtgccggagg tgttcctgcc ccgcacaagc
      301 cgcgcacttc tatatcccct tcgactggtc tagaccggct ttaccgcggt atcgcgctca
      361 taacccaagg ttctccttcg attggggcat gtaagtcaga ctagctgacc cgtgaagcag
      421 tgacccaagg gtctgctcga gatggcaggg gtctcgtacg cggccgacgc tgcgcttaca
      481 gcttgacatc cggtcgcggc gcccttataa attcgaaacg ctgtttctaa gtttctcatc
      541 aaggattgct gctcaatggc gcgtgaggtc gcggggattg ttcgcgtgat gcagatcctg
      601 gagtcagtgg ccatcagtcc cgacggcatc agcgtcaggg acctgagccg cgccatcggc
      661 tgtgggaagg ggaccatctc gaagatgctg ggcacgctgg agcgccgcca ctacgtccgg
      721 cgggacgccg cgagcggcca tttcttcctg acgtggcgac tgctcgcgct cgccttcagc
      781 cattccgacc aggcggggat gccgcgggtc ttcctgcccg tgctgcagga gctggccgac
      841 gagaccgacg agctcgtcca gctcgccgtg gtcgatggcg accaggtgct cttcgtggcg
      901 aaggccgagg gccgcggaca gaccatccgt ctcctgcccc tggtcggcct ctgggctccc
      961 ctgcacgcga cggcggcggg gaagctatgg ctctcgacgt tgcccgccag cgaggtatct
     1021 cgcgtgctcg gacgccggct gccggcagtc gcccctagga ccatcacctc gaagggcgct
     1081 ctccggaagg agctcgagcg cgtccaggcc gccggatacg ccctggccga tgaagagctg
     1141 gccgagggcg ggcgggccat cgccgcgccg atcaagcggg aaggcgcgat ggtgggtgcg
     1201 gtcgcgctct ccggtcccgc ctaccggctc tcgctcgagc gactgcacac gctcgctcct
     1261 cgggtgatcg aggcggcggc gcggctcgcc tcgatctggc cggcccacgt aaacgcgaag
     1321 gacttcacct cgtgagccgg ttcgagtacc accagcccga gagcctccac gacgcggtcg
     1381 ccctcgccgc gcgtttcggc gcggaggcga gcttcctcgc cgggggcacc gacctgatcg
     1441 tgcagatcgg acgcgggcgg atcgcaccac gccacgtcgt cggtctccat cgcgtccccg
     1501 ggctcgtcgg catcgagacc aatggccgta tcagccttgg ggcctgcgtc acccatcgct
     1561 ccgtcgagcg ggcgccggcc ctggccggtg cactccgttg tctcatcgag ggggccgagg
     1621 tgatcggggg gcatcaggtg cgcaacgtcg ccaccgtggg tggcaatctc gtgaatgcct
     1681 cgcctgcggc ggacctcgtg ccgtgcctgc tggccctcga cggtgcggtg acgctgctcg
     1741 ggccgggcgg cgagcgggac ctgcccatcg agcgttttct cctcggcccc aaccggacgg
     1801 cccgccagcc cgatgagctg ctgacccgtg tgagcctgcc cgcgccaccc gcgcgcgcgg
     1861 gcaccgcgtt cctgaaggcg ggccggcggc gcgcgatgga gatctcggtg gtgtgcgtcg
     1921 ccgcccgcct taccctcgac gcgagcctcg agcgctgcct ggaggcgcgc gtcgccctcg
     1981 gtgccgtcgc gccgacggcg gtgcgggcgc acgaggcgga gcgcgccctc gagggccagc
     2041 ccgtcggcgg cgaggtgttc cggcgcgcgg cggaggcggc ccaggaggcg tgccgcccca
     2101 tcgacgacgt gcgggcatcg gcggcgttcc ggcggcatct ggtcggcgtc ctggtccgtc
     2161 gcgccctcga ccgctgtgtc gagcgcgcgc gaagccacct agaccaaacc gcctagacgc
     2221 tgaggagagc ccatggcgag gattccaatc acgctggaga tcaacgggga gcgccacgag
     2281 gggcttgtcg agcccgaccg cacgctgctc gacgtgctac gctcggacct cggcctcacc
     2341 gggacgaaga ccaactgcct cgacgcggag tgcggggtct gcacggtact cgtcgacggg
     2401 ctggccgtga actcatgcct gtacctggcc gtggtggccc agggcagggc gatcacgacc
     2461 attgaggggc tcgcgccggc tcgcgagggc ccgacgcccg ccggcacgct ccatccgttg
     2521 cagcaggcgt tcgtggagca cggcgccgtg cagtgtgggt attgcatccc gggtatgatc
     2581 ctcgccgcca aggcctacct cgacgaccac ctgcagccga gcgagaccca ggtccgggag
     2641 gcgctggccg gcaacctgtg ccggtgcacc gggtaccaga agattgtcga cgcagtgttg
     2701 gccgcctcga gccgcacggc ggccgccggg acggtggtgt cgtgagcggg gccacgcggg
     2761 tcgtcggcca ctccctgccg cgcatcgatg cgccgggtaa ggtgactggc agtgcgatct
     2821 acgctgcgga cttcgccctg cccgggatgc tctacggcaa ggtcttccgg agcgccgagc
     2881 cgcacgcacg cctcgtccgc ctcgacacca gccgcgcggc ggacatcgcc ggcgtgcgcg
     2941 cggtcatatc ggcagccgac gtgcccgacg tccgctacgg ggccgccgtg aaggacgagc
     3001 cggtgttcgc ctcggacatc gtgcggtacg tcggtcagcc ggtggccgcg gtggccgcgg
     3061 tcacgctgga ggcggcggac gcggcgctgg ccacgatcga ggcggtctac gagccgctcc
     3121 cgcgggtgct cgacctggcc gccgcgctcg cccccggcgc gccgctggtg cacgagggct
     3181 ggcaggccta caccgcgatc ccgatcctcc agcgcgacgg caacgtgtgc aatcgcgcgc
     3241 ggatcgtcgt cggcgacgtg gagcgcggct gggccgaggc cgaccgggtg ttcgagcacc
     3301 ggttcaccac cgcgatggtc caccagggct acaccgagcc gcgcgcggcg gtggccgcgt
     3361 gggacagctc gggccaggtg acggtctggt cgaacaccca gctcccgttc gacgtgcagg
     3421 ccaccctggc cgacgtcctc gcgatcgcgc cgtccaagat ccgcgtgatc gtgcccgggg
     3481 tcggcggcgg gttcggcggc aagctgcggg tcggggtcga gcacttcgcg gcgctgctcg
     3541 cccggaaggc gggccggccg gtgaaggtga tgacgaccag cgaggaagag ctcaccgcgg
     3601 cctacccgcg gcagggcacg atcatcgagc tcaagaccgg ggtgacgcgc gacggccgcc
     3661 tcaccgcgcg ccaggggcgc atctacttcg acaccggggc cttcgcgggg tcggggccgg
     3721 gcgtggcgtc ggtggcgacg ctggtcctgg ccgggccgta tcgcacgccg aacctgcatc
     3781 tcgagggcta cgcggtctac accaacaaga ccaactgcgg ctcctaccgg gcgccctcgg
     3841 ggccccaggc caacttcgcg gtcgagtcgc agatggacat catcgcggac gcgctcggga
     3901 tcgacccgct cgagctgagg ctgcgcaaca tcgtccccga gggcgacgag gggccgaccg
     3961 gccaggtgct gagcgcggtc gggctcgagg agtgcctgcg gcgcgccgcc gacgcgatcg
     4021 gctggcggga ccgccggccg ggacccggcc ggggcaaggg cctggcgatc ggctggtgga
     4081 cgaccaccgg cggttcgtcc ggggtgtacg tgaagatcaa ccccgacggc accgtggcgc
     4141 tgaacaccgg cgcggtggag atcggcaccg cggccctcac cggcgcggcg caggtcctgg
     4201 ccgaggtcct cggcgtggac ctggccgaca tcacggtggt gtccggtgac accttctcga
     4261 ccccgttcga cttcggcgcg caggggagcc ggaccgcctt cgcggtgggc aacgcctgcc
     4321 gcgctgccgc ggcggagctg cagcgcaagc tgttcgcgct ggccgcggcc cagctcggcg
     4381 ccccggagga ggcgctcgtt ctgcgcgaca agcacgtggt tggcccgggg ggccggatgt
     4441 cgctcgccga gctcgcgcgg cagtcccagg tcgcgggcgg cgggatgatc gcgcacggca
     4501 cattcatcgc gccggccacc gcctacgaca ccaagcgcgt cgagggccac gtctacccgg
     4561 cgttccactc gccgagcttt cacgcccacg cggtggatct ctcggtggac gcggagaccg
     4621 gcgaggtcac catccacaag tacgtggtgg ctcaggacgt gggcttcgcc ctgaacccca
     4681 cctacatcga ggggcagatt gagggcgggg tcgcgcaggg gctcggccag accctctcgg
     4741 aggagatcgt gtaccgcgac gggcgcgtgc agaacgccaa cctcaccgac tacaagatcc
     4801 cgaccacgct cgacgtgccc cgcgtcgagt ccatcctcgt ggagcacccg ggccgggtcg
     4861 ggcccttcgg ggccaagggc gtgggcgagc cgccgaacgt cgagcccccg gccgcggtgg
     4921 ccaacgcgat cgcggcggcg gtcggggccc ggatcaccag cctgcccatt accgcggaga
     4981 agatcgtcct cggctcccac gggggcgccc gctgatgccg ctcacgaccg ggctgctcat
     5041 gctgccctcg cacccggcgg gccggctggt cgagatcgcc cagctcgcgg agcgcaccgg
     5101 ctacgactac ctctggctgg ccgacgaacg attcttccgc gaggtgtacg cctcgctgac
     5161 cctgtgcgcg ctgcgcaccc agcggatcaa gctcgggccg tgcgtgaccg atccctactc
     5221 gcgccacgcc gcgctgaccg cgatggccat cgcgacgctc gacgagatct ccgggcagcg
     5281 cgcggtgctc ggcatcgggg ccggggtgag cggcttccgc gagctgggga tcgcccgcga
     5341 caagcccggc tccggcctgc gcgaggcggt ggaggtcatc cgcaagctcc tggccgggga
     5401 gaccgtgacc tacaaggggg cggtcgtgca gatcgagggc ggccgtctcg atttcaagcc
     5461 gatgcgggcg gacgtgccga tgtacatcgc ctcccagcgg ccggtggggg tccgcgcggc
     5521 gggccgggtg gcggacggag cgatcatgca gggctgcgtc gccgagccgc tgctcgcctt
     5581 cttccgggac accgtggccg cgggggcgcg cgaggccggc cgcgatccgg cgcggatcga
     5641 cctggtggcg cggatcaacg tctgcgtgca cgacgaccgg caggtggcgc gcgacctcat
     5701 gaagcccacc atcgtgcgga gcctggccgc gcagcgcccg gacttcttca cgttcaagac
     5761 cgcgggggtg acggtgccgc ccgcgctcgc ccggcatctc gaggggctca gctacgccta
     5821 cgatccgcgg cccttccagg cggcggcggc cgaggtgccg gagagtttcg tggacgcggt
     5881 caccctggcc gggcctcccg acgaggtggc cgcgggcgtc gcgcgcatgg cgcggcaggg
     5941 ggtcacccag ttcatgctct acccgctgtc gcccgacggc cgcgtcgagc tggtaatcga
     6001 gcgcttccag agcgaggtca tgccccgggt gagggcggcg gtccggtgac agtcacggtg
     6061 gtgatgcacg gcacgctgcg gcgcttcctg ccggaggggg cggcccgcgc caccctcgac
     6121 ctgcgcgcgg gggccacgat cggggagctc ctcgtcgagc ttggggccga cgccgacacc
     6181 tggctcgtcg cgcgcaacca ggcggtcgcc gagcgggacg cggtgctggc gcccggcgac
     6241 gtgctcgact gcttcgaacc cctggccggc ggcgcgggtg ccggagacgg cggacgggag
     6301 gcgagcccat gccggtacgg tacgtgatca cgacgctcaa accgggcgtc aagcccgagg
     6361 actacgagcg ctggctggtg gagtacgact acaaggtagc gaagtcgcta ccgagcatcg
     6421 tgagctatcg cacgcatcgc atcgaagggc ccatcaacgg cgcggagacg gcgggctgga
     6481 gctacatcga gcgaatcgag attcgcgacg tggaccagta ccagcgggac atcgcgtccc
     6541 ccgcgggtca ggagctgatg cgtcagctct acggcaacta cctggagcgg tcgaagaaca
     6601 tctttttctg gtccgaagag atcacggact gaggggaggc gcacatgggc gcacggggag
     6661 caggggtcag ccgacgcgac tttctggtga ccgcgggggt cggggccgcc gggctcgcgg
     6721 tgggacggcg ggcggcggcg caggacaaga agacgctggt ggtggcgtgg gactcggaca
     6781 tcgattccct cgaccccgcg gtgttcaagt cgcagggcgc ctacgtcacg gtggccaaca
     6841 ccaccgacgc gccaatcatg tggaaggtgc ggccgctgga cggcaagccc gggcttttcc
     6901 gctcgatgcc ggcggagtgg gacgggcacc tggccgagtc gtgggcgatg gaggacaacg
     6961 gggccacgat cgtgctcaag gtccgccgcg gcatgaagtt cccgagcggc cggcccatca
     7021 ccgcgcacgc cttcaagtac tcgttcgacc gcggcctgct ttcgccgggg tacatgaagc
     7081 tcatcttccc gaccctgatc caggtgagcg cgccggagca gttcgtggtg cgcgacgact
     7141 acacgttcgt catcaacatg aaggcgccga gcccgatggg cctcgacacg gtggcgctgt
     7201 ccaacaatgc catcctcgat ccggacgtgg tcaaggccaa cgggaccaag gacgatccct
     7261 gggctaccga gtggctcaag cgtaacgggg cgtcggtcgg gccctaccgg ctcgtcaaga
     7321 acgagccggg cgtcgagatc gtgatggagg ccaccccggg ctactggcgg cccaagccct
     7381 acttcgaccg ggtgatcctc aagctggtgc ccaacgaggc cgatcgcgtt cttctcctca
     7441 agcgcaaggc ggtggacctg gtggcgggtc ggccgggcct gagcccgaag aacgtgaagg
     7501 cgctcgaggg cgaggccggg cttaaggtgt tcagcgtgcc cgacaccacc tgccactacc
     7561 tctgcatgaa ccagaagaag cccccgttcg acaacaagct ggtccggcag gcggtcaact
     7621 acgcgatccc gatccaggcc atcctgccca acgtgctgtt cgggtacggg acccagatga
     7681 agagcccggt gcccaacctc accccgagcc acctcggcga ctactcgccc tacaagcacg
     7741 acatcgcgaa ggcgaaggcg ctgatgaagg aagccggcat ggacaaggcg ccgatcccgg
     7801 tggacctggc catccgggtg ggctggccca cgcacgagca ggcgtcggtg tggctgcagc
     7861 gggagctgga gcagatcggg ttcaaggtga acatcgtcaa ggagaccgac gcgacattcc
     7921 ggcagctcgc caccaagggc gatcaccatc tctcgatcga ggcgtggcag tcgtggatca
     7981 acgatccctt ctatcacctc acgttcctgt tcaactcgaa gtccaagttc accaacctga
     8041 gcttctacgc gaaccccgcg gtggacaagc tcatcgacga caacatgcac gagaccaacc
     8101 gcgacaagcg cgcggccgcc tcgcgcgaca tccagaagct cgtgctggac gacgcggtgt
     8161 ggggcctgct ctggtacgag aactggaccc gggtgggcac ctcggacctg gtcggcctcg
     8221 agaagcgctg ggacaccttt gagcggtact acaacctcaa gcgcgcgtag acgggcctcc
     8281 gccggccgcg cgccccgccc gatcgggcac gggcgctgcg gtcggcaggg gtgaggcccc
     8341 aacggcatgc gcatccgcgt caacggggtc gagctcttcg tggacgtgca cggccccgcg
     8401 ctcgcctggc gggacggggc caccgtcgag aagccggtcc tgctcgccct gcacggcggc
     8461 cccgggctcg atcacgcgaa cttggtgccg tggcttcagc cgctggccga gcgggcgcag
     8521 atcctgtacg tggatcaccg gggcaacggc cgctccggcc ggccacccct cgaggagtgc
     8581 acgctcgagg cgatggccga cgacctcgag gccctgcggc ggctgctcgg gctgggcccg
     8641 ctgatcgtgc tgggcgtgtc cttcgggggc atggtggccc tcacgtatgc cctgcggcat
     8701 ccggccgggc tggcccggct gatcgcgtgt gtgaccgcgg cgagcggcga cttcgtggcc
     8761 gacgcccggc ggcttgcccg ggcgcgggcg acgccgcggc agatggaagt ggtggagcac
     8821 atgctggacg gcacctttcg cgacgaggcg cacttccgcg aggggttcgc ggcagtcgcc
     8881 tcgctgtacg agcaccgctc ggatccggcg cgcgacgcgg ccgccctgcc gagcgtgctc
     8941 aacgtggcga tgctcaactg gttcttcgcg acggaggtga agcgctacga tctgcggccg
     9001 cgcctcgggg aggtgcgggc gcccaccctg gtcctgaccg ggcggcacga ctggatctgc
     9061 gcccccgcgc actccgacac cctggtccgg gggatcccgg gcgcgcgcca ggtgatgttc
     9121 gaggagagcg ggcaccgcgt gatcaaggaa gagaacgcgc ggttcgtccg ggtggtcacc
     9181 gagttcatcc acgagacgac gtgaggagac cgagtccatg atcgaagcgc ccaaggggta
     9241 cgagcccgat ttcccaccgg ttttccaggg cggcagcccc gaggatcacg ccgccctgct
     9301 gcgcctctac ttcgacctgc ggctcgccaa cgacgggctc gacaacgacg cgctccgcaa
     9361 gatctgggag ccggacgccg ggcacatcta cttcaacacc aacggccacg cctaccacgg
     9421 cctcgaggac tggctgcaca tctgggacca ctaccggac
//
LOCUS       DASUFA010000259         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_67362, whole genome shotgun sequence.
ACCESSION   DASUFA010000259 DASUFA010000000
VERSION     DASUFA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67362"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1151)
                     /locus_tag="VFX87_09625"
     CDS             complement(168..1151)
                     /locus_tag="VFX87_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX4995218.1"
                     /translation="MSDMPSHVTIKEVGPREGFQFEKGDISLAAKISLVDALSDTGVS
                     TIEFTSFVSPKWVPQMADAEQVVAGIKRVPGVAYEAIWLNEAGLERAARLRESLALRP
                     MFGVAATDTFTRKNTNRSVDEKIAELPGWARRYQALGMDWIDVGVMAAFGCNYEGDVP
                     PAQVITVLEKAEEQAVGCGSRLGNISLADTMGWANPLQIKRLVGAVRARWPAIPVKLH
                     LHDTRALAIANVVAALEIGVDRFDSAVAGLGGCPFAAHKGAAGNVTTEDLVFLCQEMG
                     VHTGIDLDSMIEAGQLAERVVGHPLPGKLKSGGNLDKYRARARAAAPAASA"
     gene            complement(1148..2344)
                     /locus_tag="VFX87_09630"
     CDS             complement(1148..2344)
                     /locus_tag="VFX87_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4995219.1"
                     /translation="MTTPLPLEGVRVLELGHIIAGPSAGLLLADLGADVIKVERPGDG
                     DQSRNMPAGTSANFHFLNRNKRSIAIDLKGSAEGRALFLRLVAGADVVIDNFAYGAVE
                     SLGLGYDVLARVHPGIIYLALKGFLPGPSEARPFLDELAQMSAGLAFMTGPRGQPLRA
                     GASIVDVGAAAYGVIAVLAALHQRAQTGRGQKITSGLFETVVFWVGQWLAQHAATGQP
                     SVPMPEIRQGTRMGWGVYQLFTAADGEQIFIGITSNGHWERFCDEFGLKDLLADERLD
                     DNAKRVAARAWLPARIAEEMLRYSSAELSDRLERCRVPFAPLRRPDQLLDDAHLLATG
                     QLLDTPLPGGGVAKLPKLPVHSTAFDMGLQRPAPTLGEHTREVLRELGLETDEIDALA
                     SRRVIG"
     gene            2451..3002
                     /locus_tag="VFX87_09635"
     CDS             2451..3002
                     /locus_tag="VFX87_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995220.1"
                     /translation="MPDEARRWLWILLLTAASVFVTVGMACATPFAALATLAALHMSR
                     RDGLALIGIAWLADQTVGYGLLGYPRTANSLAWGVILGVGAVVALLAARALADRFRGR
                     GGVLAGGLAFAGAFVAHQAVLFAATAALASGAEAFSLPIVGWVLRVNLLSIAGLLILS
                     RLAVWIGLVTRSPLGARATVTAG"
     gene            3007..>3571
                     /locus_tag="VFX87_09640"
     CDS             3007..>3571
                     /locus_tag="VFX87_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995221.1"
                     /translation="MSVTGKLDRPGGSLHFEVIDATAPWAGEAPLIVFHHGIGATAEV
                     WAEWLPALLDRYRVARFDMLGFGRSGVPGPGFGWSLEGLMADALEVARAAGAERFHFV
                     GESVGGTVGLLLGSRRPDVLLSLTVSNASHRGGSIQRAREWREFIRVNGMVRWGEMMA
                     PLRLDRDRVSEARYRWFEQAQARCSADS"
BASE COUNT          453 a         1234 c         1341 g          543 t
ORIGIN      
        1 gagtgggcca cgtggtgctg gggaagaccg tctccaggcc cgaggcgagg gcaccgcgct
       61 cggcgagacg ccggaggcgc ggcaggcggg cctggggatc cgcccagtag aacgccgcga
      121 agccgtcgat gctgatcacg acgacgcggc gcttcacggg gggcgcgtca ggccgacgcc
      181 gcgggggcgg ccgcgcgggc gcgcgcgcga tacttgtcga ggttgccgcc cgacttgagc
      241 ttgccgggca gcgggtggcc gaccacgcgc tcggcgagct gtcccgcctc gatcatcgag
      301 tcgaggtcga tgccggtgtg gacgcccatc tcctggcaga ggaagaccag gtcctcggtg
      361 gtgacgttgc cggccgcgcc cttgtgggcc gcgaacgggc agccgcccag gcccgccacc
      421 gcgctgtcga agcggtccac gccgatctcg agggcggcga ccacgttggc gatggcgagc
      481 gcgcgcgtgt cgtggaggtg gagcttcacc gggatcgcgg gccagcgcgc gcgcaccgcg
      541 cccaccaggc gcttgatctg caagggattc gcccagccca tggtatcggc gaggctgata
      601 ttgccgagcc gcgagccaca gcccaccgcc tgctcctccg ctttctcgag gacggtgatc
      661 acctgcgcgg ggggcacatc gccttcgtag ttgcagccga acgcggccat cacgcccacg
      721 tcgatccagt ccatgccgag ggcctggtag cgccgggccc aacccggcag ctcggcgatc
      781 ttctcgtcca cgctgcggtt cgtgttcttt cgcgtgaagg tgtcggtggc cgccactccg
      841 aacatcgggc gcaacgcgag cgactcccgc aggcgcgccg cgcgctcgag ccccgcctcg
      901 ttgagccaga tcgcctcgta ggcgacgccg ggcacgcgct tgatgccggc caccacctgc
      961 tcggcgtcgg ccatctgagg gacccacttc gggctgacga aggacgtgaa ctcgatggtg
     1021 gagacgccgg tgtcggagag cgcgtccacc agggagatct tggcggcgag gctgatgtca
     1081 cccttctcga actggaagcc ctcgcgcggg cccacttcct tgatcgtgac gtggctcggc
     1141 atgtcgctca tccgatgacc ctccgtgacg cgagtgcgtc gatctcgtcg gtctcgaggc
     1201 ccagctcccg cagcacctcg cgggtgtgct cgcccagcgt gggcgccggg cgctggagcc
     1261 ccatgtcgaa ggcggtgctg tggaccggca gcttgggcag cttcgccact ccgccgccgg
     1321 ggagcggggt atccaggagc tggccggtcg cgagcaggtg cgcgtcgtcc agcagctgat
     1381 cggggcgcct cagcggtgcg aacggcaccc ggcagcgctc gaggcggtcg gagagctccg
     1441 cgctcgagta gcggagcatc tcttccgcga tgcgggcggg tagccaggcg cgtgccgcga
     1501 cgcgcttggc gttgtcgtcg aggcgctcgt ccgcgagaag gtccttgagc ccgaactcgt
     1561 cgcagaagcg ctcccagtgg ccgttggacg tgatgccgat gaagatctgc tcgccgtcgg
     1621 ccgcggtgaa gagctgatac acgccccagc ccatgcgcgt gccctggcgg atctcgggca
     1681 tcggcaccga cggctggccg gtggccgcgt gctgggcgag ccactggccc acccagaaga
     1741 cgacggtctc gaagaggccc gaggtgatct tctgcccccg gcccgtctgc gcccgttgat
     1801 ggagcgcggc gaggactgcg atgaccccgt aggccgcggc gcccacgtcc acgatcgagg
     1861 cgccggcgcg gagcggctgg ccgcgcggcc cggtcatgaa ggccagcccg gccgacatct
     1921 gcgcgagctc gtccaggaag gggcgcgcct ccgacggacc ggggaggaag cccttgagcg
     1981 ccaggtagat gatgcccgga tgcacccgcg ccaggacgtc gtagccgagg cccaggctct
     2041 cgacggcgcc gtaggcgaag ttgtcgatca ccacgtccgc gcccgcgacc aggcgcagga
     2101 agagcgcccg gccttctgcg gagcccttga gatcgatcgc gatcgagcgc ttgttgcggt
     2161 tcaggaagtg gaaattggcc gaggtgcccg ccggcatgtt ccgcgactgg tcgccgtcgc
     2221 cgggccgctc caccttgatc acgtcggcgc ccaggtcggc caggagcagc cccgccgagg
     2281 gccccgcgat gatgtggccg agctcgagga cgcgcacgcc ctcgagcgga agcggggtgg
     2341 tcatgacgcg tcattgtatc gtaaacgcag gtgccctggt tgcgcggcca ggaccgctct
     2401 ggtaccgtcg gtcgcgctgg cagcctcgac gacacgagag gagtaacgtg atgcccgacg
     2461 aagcccggcg ctggctctgg atcctcctgc tgaccgcggc gagcgttttc gtcacggtcg
     2521 gcatggcgtg cgccacgccc ttcgcggcgc tcgcgacgct ggccgcgctc cacatgagcc
     2581 ggcgcgatgg gctcgcgctg atcgggatcg cctggctcgc cgaccagacg gtgggctacg
     2641 gcctgctcgg gtacccgcgc accgcgaaca gcctcgcctg gggcgtgatc ctgggcgtgg
     2701 gcgccgtcgt ggcgctgctc gcggcccggg cgctggccga tcgcttccgc ggccgcggcg
     2761 gggtgctggc gggggggctg gcgttcgcgg gggcgttcgt ggcccaccag gcggtgctct
     2821 tcgccgcgac cgcggcgctg gcgagcgggg cggaggcgtt ctcgctgccg atcgttggct
     2881 gggtgctccg tgtgaacctc ctctcgatcg cgggcctgct gatcctctcc cggctcgcgg
     2941 tctggatcgg gctcgtgacg cggtcgcctc tcggggcgcg ggccaccgtc acggccggct
     3001 gatcccttga gcgtcaccgg caagctggac cggccgggcg ggtcgcttca cttcgaggtc
     3061 atcgacgcga ccgcgccgtg ggcgggggag gcgccgctca tcgtcttcca tcacgggatc
     3121 ggggccaccg ccgaggtgtg ggcggagtgg ctccccgcgc tgctggaccg ctaccgggtc
     3181 gcccgcttcg acatgctggg cttcggccgc tccggcgtgc ccgggccggg cttcggctgg
     3241 tcgctcgagg ggctgatggc cgacgcgctc gaggtcgcgc gggccgcggg cgccgagcgc
     3301 ttccacttcg tgggcgagtc ggtggggggc accgtcgggc tgctgctcgg gagccggcgt
     3361 cccgacgtac tgctgagcct gaccgtgtcg aacgcctcgc accgcggtgg ctcgatccag
     3421 cgggcgcgcg agtggcgtga gttcatccgt gtcaacggca tggtcaggtg gggcgagatg
     3481 atggcgccgc tccgcctcga ccgcgaccgc gtctccgagg cccggtaccg ctggttcgag
     3541 caggctcagg ctcggtgctc cgcggactcc g
//
LOCUS       DASUFA010000260         8377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1557405, whole genome shotgun sequence.
ACCESSION   DASUFA010000260 DASUFA010000000
VERSION     DASUFA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557405"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..821)
                     /locus_tag="VFX87_09645"
     CDS             complement(99..821)
                     /locus_tag="VFX87_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4995222.1"
                     /translation="MLSVLRPAAPAASRGVLVETFPPVYRVFRGVAGLVLRELFDLRV
                     EGAERLPVSGPFILASNHHNYLDGVVLGVAVPRPIAFLVMPRVFRATPLHPPFHRRIG
                     SIPVALERPDPGAIKRVLQVLDAGRVVGIFPEGPFSQEGRLVRGRPGTAMVALRAGVP
                     VVPAAIEGTYEALRGRRFYLPRRRPLTVRFGEPVHFGRARTGPIPRTEREEITRRIMS
                     EIAALLRVPPAPAAAAGRAGAS"
     gene            complement(1020..2000)
                     /gene="mltG"
                     /locus_tag="VFX87_09650"
     CDS             complement(1020..2000)
                     /gene="mltG"
                     /locus_tag="VFX87_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX4995223.1"
                     /translation="MSFVVVVALLSLLLEGWWILSVPAPLRASARVVELPAHKSVIEV
                     AEILDEAGVIRSRVGFVLLALARGSFRSLKAGEYQIPAGSNTVRVLEQIAGGQVLQHM
                     VVFPEGSTLAELARLLQTERLVSGDDILRVGKDPVFLKNLDVQADSVEGYLFPDTYQF
                     VKGMTPEEILARMVARMREKISPELLAEARARDVSLHQMLTLASIIEKEAVESSEMPL
                     ISAVFWNRIRRDMPLQADPTVQYAVGKDRKRLTREDLQVDSPYNTYRRQGLPPGPIAS
                     PGRAAIAAAVRPARVNYLYFVSLDERFHTFSTNLADHNAAVARYRLARSR"
     gene            2211..3209
                     /locus_tag="VFX87_09655"
     CDS             2211..3209
                     /locus_tag="VFX87_09655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX4995224.1"
                     /translation="MQELVLPVRHEWLARERAYGKIAIEPFEPGFALTTGNAYRRVLL
                     SSITGAAPTWVKIEGVLHEFSHLVGMREDTLDIILNLRKLVFTLHVNRPKLLRLKAQG
                     VRTVTARDFEPDPDVEILTPDVPLATLDKDGSLEMEVCVERGRGYQPAEKREPEALPI
                     NAMMMDADFSPVKRVNFHVEPKGAQERLIMEVWTNGTVTPEVAVGDASRILDDQFALL
                     IDFPSTPPAQPEGTEAVAGEPAPRSEVNEHLFRNVDELELSVRASNCLKTANIRTIAD
                     LVQKTESELLKTKNFGKKSLNEIKTILGEMGLSLGMRLDPEELERLRAHYERAFET"
     gene            complement(3251..5647)
                     /locus_tag="VFX87_09660"
     CDS             complement(3251..5647)
                     /locus_tag="VFX87_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX4995225.1"
                     /translation="MIERRLASAPLLPPTVAFTAGIAAAAWAPLPAPRLLAAATAILL
                     VAALASRRHPRATVLLVLAGLALLGALRASGSPLPADHLARRALVGTPTLEGRLAEEP
                     VRWTSDRTRLLLDVDALHAGPERLPAVGRVQLTVYGELALPLGETQRVLVDARLHRPI
                     GFRNPGGFDYPAHLRREGILLVGHARADRIIALTADAPPWPVAVKRWAIGVIAARLPE
                     TSAALLAGLLLGERSTLPREIDESFRRAGVYHILAVSGFNVALLAGAVFGGLAMCGVP
                     RRGAAAAAALVLVGFALVVGGQPSVLRATVMGLLLLAAMLLDRESQLPNALALAVLAL
                     LLWRPGDLWEPGFQLSFAATAGIVYLGPAIAARLVARGAPAWLAGAVAVSLGAQAAVT
                     PLMLAHFNQLSLIGVVANLLVVPLAAVATTLGMLALLVELASGALAALLFHTLWVILI
                     ALRAGVSLAAAFPAAMIHLPAPTAATAAAWYGALLLAPGMVASRRIRVVVGVLALLVA
                     TLSIWPWLRPTETMLRVTFLDVGQGDAALIELPEGPRLLVDGGPGGGRRFDVGERVLA
                     PFLWNRPLARLDAVALTHWDTDHSGGLAAVLAHFYVGEFWESGRPPGGAPETVAALLR
                     SRTPRRVLAAGQRLWLGRALITVLGPGPDSTQTPNDQSLVLRLDWRGVSLLLAGDLGP
                     RGEALLLERGGPVSALALKVAHHGSRFSSTAPFLERARPRLAVISVGTRNPFRHPSAE
                     ALARLEAAGARVYRTDRDGAIILETDGRVLRVTAWARGATETWELDPDESSENRTAPG
                     "
     gene            complement(5640..6080)
                     /locus_tag="VFX87_09665"
     CDS             complement(5640..6080)
                     /locus_tag="VFX87_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX4995226.1"
                     /translation="MLYSVPQLRLLILLALTLLVGLGVREWRAGFPDAALRLERFDRD
                     EPAVPLVGETPSSAPSPPRAPARVTPPPPAAPDPPPPKPLDVNRAAAAELAALPGVGP
                     GLAQRIVEERERRGRFDSPEALRYVLGMGPKKLAAIQRFITVSD"
     gene            complement(6491..7327)
                     /locus_tag="VFX87_09670"
     CDS             complement(6491..7327)
                     /locus_tag="VFX87_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4995227.1"
                     /translation="MSKTIADLIQERFGLPTEAGRARPAEGALALLLSHRTQRRYKPD
                     PIPDDVLEMALAAALSAPSKSDLQQVGIVLVRDRAKQTTIGGWIPAMPWIAQAPLFMV
                     FCGDHRRIRRVSELRARPFPNDTLDMFMNAAVDAGLVLQAFITAAEALGLGCCPISVV
                     RNHVERLATLLELPPGVFPVAGLCVGYPREPGATSMRLPPAVTVHTDRYDDADLPAYL
                     AAYDARREARQATPKENQRFVERFGYAEPYSWSEDKARQYSVPERHNFGPFIRRHGFG
                     LA"
     gene            complement(7324..>8377)
                     /locus_tag="VFX87_09675"
     CDS             complement(7324..>8377)
                     /locus_tag="VFX87_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4995228.1"
                     /translation="VPIPEVEPEGVLIRVSHANICGSDLHFWRGDAPLRLPDDGWIFG
                     HEMTGRVARLGAKIKTDSLGRPLKEGDRVAYTYFYPCGRCYACLHQEPAACPNKLERP
                     LGPSAFPHLHGAFADHYYLRPRGEIFLVPDSLSDEAVAGVNCALSQVFYGLHVAGLRQ
                     GDSVVIQGAGGLGIQAAAVAKDMGAQTVIVVDQIPGRLELARAFGADHTINVKEVTDR
                     RERVKLVRQWTGGVGADVACDLVGFPAVIPEGIEMLRSGGTYLEIGTISRGAKVELEP
                     SLLVWGSKKIVGVIQYDPWVIPRALDFLTRTRESRPWHRILSHTYPLEAINEAFAASE
                     WHNRETTTITRAALTP"
BASE COUNT         1288 a         2929 c         3123 g         1037 t
ORIGIN      
        1 gtcgaaggac agggacgcgg tgaaggcttc ggcggtcgga tcggcccctt cggcgaagcg
       61 cccggaccag gccttgcccg ggggcgtacc ggcggccgtc acgacgcgcc cgcgcggccg
      121 gcggccgcgg cgggggcagg gggcacgcgc aggagcgcgg cgatctcgga catgatccgg
      181 cgagtgatct cctcccgctc cgtccgcggg atgggcccgg tgcgcgcccg gccgaagtgg
      241 accggctccc cgaaccggac cgtgagcggc cggcgacgcg gcaggtagaa gcgccgcccg
      301 cggagcgcct cgtaggtgcc ctcgatagcg gcgggcacca ccggcacacc tgcgcgaagc
      361 gcgaccatcg cagtcccggg ccgtccgcgc acgagacggc cttcctggct gaacggaccc
      421 tccgggaaga tacccaccac gcggccggca tcgaggacct ggagcacgcg cttgatggcg
      481 ccgggatccg gccgctcgag cgccaccggg atcgagccga tccgccggtg gaagggcgga
      541 tgcagtggcg tggcccggaa cacgcgcggc atcacgagga acgcgatggg acgcggcacc
      601 gcgacgccta gcacgacccc gtccagatag ttgtgatggt tcgaggccag aatgaaaggc
      661 ccggaaacgg gcagccgctc cgctccctcg acccgcagat cgaacaactc ccgcaggacc
      721 aaccccgcca cgccccggaa cacccggtag accggcggaa acgtctccac gagcacaccg
      781 cgcgacgccg cgggcgcggc cggccgcaac acagacagca tggaggagtc ttcgcccgag
      841 cccataactc gctccttata gcatccaccc cccacgctga caaccgcacc ccggagcctg
      901 ccggccaagc tcgggacgct cacaaccgca tgatgatcgg ggacgacctg agggaaacga
      961 gagggcgggc gggggcggcg gcactccggc ccgcccggtg gatgccgacg cggggagggc
     1021 tagcgagagc gcgcgaggcg gtaccgcgcc accgccgcgt tgtgatccgc caggttggtc
     1081 gagaaggtgt ggaaccgctc gtccagcgag acgaagtaga ggtagttcac gcgtgccggc
     1141 cgcaccgccg ccgcgatggc cgcgcggccc gggctcgcga tggggccggg cgggaggccc
     1201 tgacggcgat aggtgttgta aggcgagtcc acctggagat cctcacgggt gagccgcttg
     1261 cggtccttgc ccaccgcgta ctgtaccgtc ggatccgcct gcagcggcat gtcgcgccgg
     1321 atccggttcc agaacaccgc ggagatgagg ggcatctcgc tggattccac cgcctccttt
     1381 tcgatgatgg aggcgagcgt gagcatctgg tgcaggctca cgtcgcgggc gcgcgcctcc
     1441 gcgagcagct cgggcgagat cttctcgcgc atccgcgcca ccatgcgggc caggatctcc
     1501 tcgggggtca tgcccttcac gaactggtag gtgtccggga agaggtagcc ttcgaccgaa
     1561 tcggcctgga cgtcgaggtt tttcaggaag acggggtcct tgccgacccg gaggatgtcg
     1621 tcgcctgaga ccaagcgctc ggtctggagc agccgcgcca gttcggccag cgtgctgccc
     1681 tccgggaaga ccaccatgtg ctggaggacc tggccgcccg cgatctgctc gagcacccgc
     1741 accgtgttcg agcccgcggg gatctggtac tcaccggcct tgaggctgcg gaagctgccc
     1801 cgggccaggg ccaggaggac gaagccgacc cggctccgga tcactcccgc ttcgtccaga
     1861 atctcggcca cttcgatgac gctcttgtgg gcaggaagct ccacgaccct ggccgaggcc
     1921 cggagcggag ccgggacgct caggatccac cagccttcca ggagcaggga gaggagggct
     1981 acgaccacca cgaagctcac ggcggccctc caggtcgggc cgggacgtcc cggcccaacc
     2041 ccccatgtcg gaagcgatcg ggggcgttcc atcatggtca accttcattc tggccggact
     2101 gggattctca gtcaaaccgg gggggatggg gaaggctggc ggcctgggag cttcctggta
     2161 taatgaccgg cctggggcga cccgccccag aactctgagg agggaatccc atgcaggagt
     2221 tggtgcttcc agtccgtcac gaatggctcg cccgggagcg cgcctacggg aagatcgcga
     2281 tcgagccctt cgagccgggc ttcgccctga cgacgggcaa tgcctaccgc cgggtgctgc
     2341 tgtcgtccat cacgggggcc gcgccgacct gggtcaagat cgagggggtg ctgcacgagt
     2401 tctcgcacct ggtcgggatg cgcgaggaca ccctcgacat catcctgaac ctgcgcaagc
     2461 tcgtgttcac gctgcacgtc aaccgcccca agctgctccg cctgaaggcc cagggggtgc
     2521 ggacggtgac cgcgcgggac ttcgagcccg atcccgacgt ggagatcctg acgccggacg
     2581 tgccgctggc cacgctcgac aaggacggca gcctcgagat ggaggtgtgc gtggagcgcg
     2641 ggcggggtta tcagccggcg gagaagcgcg agccggaggc gctgcccatc aacgcgatga
     2701 tgatggacgc ggatttctcg ccggtgaagc gcgtgaactt ccacgtggag ccgaagggcg
     2761 cgcaggagcg gctcatcatg gaagtgtgga ccaacgggac ggtgacgccg gaggtcgcgg
     2821 tgggcgacgc ctcgcgcatc ctcgatgacc agttcgcgct gctgatcgac ttcccgtcca
     2881 cgccgccggc gcagccggag gggaccgagg ccgtcgcggg cgagccggcc ccgcggagcg
     2941 aggtgaacga gcacctcttc cgcaacgtgg acgagctcga gctgtcggtg cgcgcgtcca
     3001 actgcctcaa gaccgccaat atccgcacca tcgcggatct cgtccagaag accgagtcgg
     3061 agctgctgaa gaccaagaat ttcggcaaga agtcgttgaa cgagatcaag accatcctgg
     3121 gggagatggg gctctccctg ggcatgcgtc tcgatcccga ggagctcgag cggctgcgcg
     3181 ctcactacga gcgcgccttc gagacgtaga tcgcctcccg cttccctgcc gcgtccacca
     3241 cggcctccgg tcacccgggg gccgtgcggt tttcagatga ctcgtcgggg tcgagctccc
     3301 aggtttcggt ggcgccgcgg gcccacgcgg tcacgcggag gacacgccca tcggtctcca
     3361 ggatgatcgc gccgtcgcgg tcggtgcggt agacgcgcgc gcccgcggcc tccaaccggg
     3421 cgagcgcctc cgcgctggga tggcggaagg gattccgcgt gcccaccgag atcaccgcca
     3481 accgcggccg agcccgctcg aggaacggcg cggtggacga gaaccggctg ccgtgatgcg
     3541 cgaccttgag cgcgagcgcg ctcacggggc cgccgcgctc cagcaggagc gcttcgccgc
     3601 gcgggccgag atcgcccgcg agcagcagcg acacgcctcg ccagtcgagc cgcagcacca
     3661 gggactggtc gttgggcgtc tgcgtcgaat ccggaccggg gccgagcacg gtgatgagcg
     3721 cgcggccgag ccacaggcgc tgaccggccg ccagcacgcg ccggggcgtg cgcgaccgca
     3781 gcagcgcggc caccgtctcc ggcgcgcctc ccggcgggcg gccgctttcc cagaactcgc
     3841 ccacgtaaaa gtgggcgagg acggcggcca gcccgccgga gtgatcggtg tcccagtgag
     3901 tgagggcgac cgcgtcgagt cgggcgagtg gccggttcca gagaaatggc gccagcacgc
     3961 gctcgcccac gtcgaagcgc cggccgccgc cggggccgcc gtccacgagc aatcgcggcc
     4021 cctcgggcag ctcgatgagc gccgcgtcac cctggccgac gtcgaggaac gtcacgcgga
     4081 gcatcgtctc ggtgggccgg agccagggcc agatcgagag ggtcgccacc aggagcgcca
     4141 gaacgcccac cacgacgcgg atgcggcggg acgcgaccat cccgggggcg aggaggagtg
     4201 cgccgtacca ggcggccgcg gtcgctgcag tcggggcggg caggtggatc atggcggcgg
     4261 gaaaggcggc cgccagagag acgcctgcac gtagcgcgat caggatcacc cagagcgtgt
     4321 ggaagagcag cgcggcgagg gcgcccgagg ccagttccac cagcagcgcg agcatgccga
     4381 gggtggtggc caccgcggcg agcggcacca cgaggaggtt ggcgaccaca ccgatcagcg
     4441 agagctgatt gaagtgggcc agcatgagcg gcgtgaccgc ggcctgggcg cccaggctca
     4501 ccgccaccgc ccccgcgagc caggcggggg cgccgcgggc gacgagccgc gccgcgatcg
     4561 ccggcccgag gtagacgatg ccggcggtgg ccgcgaagga gagctggaag ccgggttccc
     4621 agagatcgcc cggccgccag agcagcaggg cgagcaccgc gagcgccagc gcgttcggga
     4681 gctgcgactc gcggtcgagc agcatggcgg cgagcaggag cagccccatc acggtggccc
     4741 gcagcaccga gggctggccg ccgacgacca gcgcgaagcc gacgagcacg agggcggccg
     4801 cggccgccgc gccgcggcgc gggacgccgc acatggccag ccccccgaac accgcgcccg
     4861 cgagcagcgc cacgttgaag cccgacaccg cgaggatgtg gtagacgccg gcccgccgga
     4921 agctctcgtc gatctcgcgc gggagcgtgg agcgctcccc gagcagcagg cccgcgagca
     4981 gcgcggccga ggtctccgga agacgcgcgg cgatgacgcc gatcgcccat cgcttcaccg
     5041 ccaccggcca gggcggagcg tccgccgtca gcgcgatgat ccggtccgcc cgcgcgtggc
     5101 cgaccaggag gatgccctcg cgccgcagat gcgccggata gtcgaagccg ccaggattcc
     5161 ggaagccgat gggccgatgg agccgggcgt ccacgagcac gcgctgggtc tcgccgagcg
     5221 ggagcgcgag ctcgccgtag acggtgagct ggacgcggcc cacggcgggc agccgctcgg
     5281 gacccgcgtg cagggcgtcc acgtcgagca ggagccgcgt gcggtcggac gtccagcgga
     5341 ccggctcctc cgcgagccgc ccctcgaggg tcggcgttcc gacgagcgcg cgtcgggcca
     5401 ggtgatcggc ggggagcggc gaccccgagg cccgcagcgc gcccagcagg gccaggccgg
     5461 ccaggaccag gagcaccgtc gcccgcggat gccgacggct cgcgagcgcg gcgaccagaa
     5521 ggatggcggt cgcggcggcg agcagtcgcg gcgcggggag aggagcccac gcggccgcgg
     5581 cgatgcccgc ggtgaaggcc accgtgggcg gcaggagcgg cgcgctcgcg agccgccgct
     5641 caatcactca cggtgatgaa gcgctggatg gccgccagct tcttcggccc catgcccagg
     5701 acgtaacgga gagcctcggg cgagtcgaag cggcctcgcc gctcgcgctc ctcgacgatg
     5761 cgctgggcga gccccggccc cacgccgggc aacgcggcca gctcggcggc ggcggcccgg
     5821 ttgacgtcga gcggcttggg cggcggcgga tcgggcgcgg ccggcggggg cggggtcacg
     5881 cgcgcgggcg ctcgtggtgg cgacggcgcc gaggacggcg tctcgcccac gagcggcacc
     5941 gccggctcgt cgcgatcgaa tcgctccagc cgcagcgccg cgtcggggaa gccggcgcgc
     6001 cattcgcgga cgcccaggcc cacgagaagg gtgagcgcca gcaggatcag gagccggagc
     6061 tgcgggaccg agtagagcac gccttcaccg tcccacgacg atccggcctc ggccagagtg
     6121 ccaatcggga tacgcccccg gcggcccgac ccatcgccgg gcttctcggc ggattctcgc
     6181 ttttcctgtt cttccgcctg cagcggcagc agcggagggc gccttggcgg cgggaagcgg
     6241 gcatccggag caaccggcag ctggagcggc ggacgttcgt gatcggggtc gccgcggtgc
     6301 tcgtcctggt ggccgctctg gtcgcgattc agctcgcccg gcgccccgcc cggcctgcgt
     6361 cgggcgggtc gtcatcgtcg cggggccggg tgggaccaag ctccagtgcg tctgcgagcg
     6421 tgggcgcatc ggcgcctgct tcgatcccgg gccctagtcg agcggtccgg tcggccggcg
     6481 caggccggcg tcaggcgagg ccgaagccgt ggcgccggat gaacggcccg aagttgtggc
     6541 gctccggcac cgagtactgc cgcgccttgt cctccgacca gctgtagggc tccgcatacc
     6601 cgaagcgttc cacgaagcgc tggttctcct tcggggtggc ctgccgcgcc tcgcgccggg
     6661 cgtcgtaggc cgcgaggtac gcgggcaggt cggcatcgtc gtagcggtcg gtgtggacgg
     6721 tcaccgcggg gggcagccgc atgctcgtcg ccccgggctc ccgcggatag cccacgcaga
     6781 gccccgccac cgggaagacg ccgggcggca gctcgagcag cgtggcgagc ctctcgacgt
     6841 ggttgcgcac cacgctgatc gggcagcagc cgaggccgag cgcctccgcc gcggtgatga
     6901 aggcctgcag caccagcccc gcgtccaccg ccgcgttcat gaacatgtcg agcgtgtcgt
     6961 tggggaaggg gcgcgcgcgc agctcgctca cccggcggat gcggcggtgg tcgccgcaga
     7021 acaccatgaa gagcggggcc tgcgcgatcc acggcatggc ggggatccac ccgccgatcg
     7081 tcgtctgctt cgcccggtct cgcaccagga cgatgccgac ctgctgcagg tccgacttcg
     7141 acggcgcgga gagcgcggcc gccagggcca tctcgagcac gtcgtccggg atcgggtcgg
     7201 gcttgtagcg gcgctgcgtc cggtgcgaga gcagcagggc gagcgcgcct tcggcgggac
     7261 gcgcccggcc ggcctcggtg gggaggccga agcgctcctg gatcaggtcc gcgatggtct
     7321 tgctcacggc gtgagcgccg cgcgggtgat cgtcgtggtc tcccggttgt gccactccga
     7381 ggccgcgaag gcctcgttga tggcctcgag cgggtacgtg tgcgagagga tgcggtgcca
     7441 cggccggctc tcgcgcgtgc gggtcaggaa gtcgagcgcg cgcgggatca cccacgggtc
     7501 gtactggatc acgccgacga tcttcttgga gccccacacg agcagcgagg gctcgagctc
     7561 gaccttggcg ccgcggctga tcgtgccgat ctcgagatag gtcccgcccg agcggagcat
     7621 ctcgatgccc tcggggatca ccgccgggaa gcccaccagg tcgcacgcca cgtccgcgcc
     7681 gaccccgccc gtccactggc gcaccagctt cacgcgctcg cgccggtcgg tcacttcctt
     7741 cacgttgatc gtgtggtccg ccccgaaggc ccgcgccagc tcgaggcgcc ccgggatctg
     7801 atccaccacg atcacggtct gcgcgcccat gtccttggcc accgcggccg cctggatgcc
     7861 gaggccgccc gccccctgga tcaccaccga gtcgccctgg cggagcccgg ccacgtgcag
     7921 gccgtagaag acctgggaga gcgcgcagtt cacgccggcc accgcctcgt cggacaatga
     7981 atccggcacc aggaagatct cgccgcgcgg gcgcaggtag tagtgatcgg cgaaggcgcc
     8041 gtgcaggtgc gggaacgcgc tcgggccgag cggccgctcg agcttgttgg ggcaggccgc
     8101 gggctcctgg tgcagacacg cgtagcagcg cccgcacggg tagaagtagg tgtacgcgac
     8161 acggtcgcct tctttgagcg ggcgcccgag cgagtcggtc ttgatcttcg cgccgaggcg
     8221 ggccacgcgc ccggtcatct cgtggccgaa gatccagccg tcgtcgggca ggcgcagcgg
     8281 ggcgtcgccg cgccagaagt ggagatcgga gccgcagatg ttggcgtggg atacccggat
     8341 cagcacgccc tccggctcga cctcggggat cggcacc
//
LOCUS       DASUFA010000261         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1557590, whole genome shotgun sequence.
ACCESSION   DASUFA010000261 DASUFA010000000
VERSION     DASUFA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557590"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFX87_09680"
     CDS             <1..516
                     /locus_tag="VFX87_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4995229.1"
                     /translation="PAGLMATIVELGWVPPEKAVRFGKGGKVQPAGPQITITQTHAEH
                     SSEVTVTDPATKKTTTYPGGEPAGFIVEMENGFKLYHMGDTGLFGDMRLIAEYYKPDL
                     IMVPIGGHFVMDPADAAYATKEMLKPKFAIPFHYGTFPVLKGTPQEYQAALGAATTQV
                     FPISPGDKLTF"
     gene            complement(530..1723)
                     /locus_tag="VFX87_09685"
     CDS             complement(530..1723)
                     /locus_tag="VFX87_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995230.1"
                     /translation="MRALRQIAQITLVNLRNLPQRLGTSLVVVIGIAGVAGVLVSVLS
                     MAEGFRHTLASTGREDRVIMLRAGSDAEMSSGVARDQTVLLASLPAIARDGEGRPLAS
                     AELVVMVDLPRRGQTDPNNVPFRGVQPAAFAIRDELALVEGRRFARGVREVIVGRRAA
                     QQFEGLGVGSRIAFRDSDWTVVGIFSSGGDVHESEIWADAEVAMSAFRRQGFQSLTAR
                     LVDGSDAGLAGLTTAVKRDPRFSVRVWREPEYYAKQSKVLATLINVLGYTVAAFMAVG
                     ATFGALNCMYSAIASRQVEIATLRAIGFGSAPVVVSVLIEALVLSAVGGALGGGLAYL
                     YCDGASLSTLNFDTFSQVAFDFRVTWALLGKGLLWALAIGAVGGVPPAIRAARLPVTL
                     ALRAS"
     gene            complement(1723..2889)
                     /locus_tag="VFX87_09690"
     CDS             complement(1723..2889)
                     /locus_tag="VFX87_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995231.1"
                     /translation="MRFVPLLLAGLFRKKTRTVLTLLSVTVAFMLFGLLQAVTTAFES
                     GADSADAKRLLTTARYSIIELLPISYLRRIEGVPGVVGAAHAEWFGAKYQNESNAFPV
                     FAVDPSRYLDMYPEFTVAAAEREAFAKTRTGAIAGRRLVERFGWKVGQKLPIASEIHP
                     RAGGDMNWEFDLVGVIDAEDPAVQGNTDMVLINVAHFDEARQNARGRTGWYIIRIAES
                     GQAKEIAAGIDRLFANSPDETKTQPEKEFAIGFAKQIGDIGALISRILAAVFFTILIL
                     TGNTMAQSLRERIPELAILKTLGFSDARVTVLVLAESALLLLIGGAAGMAAAVGMLPF
                     LNDSTGGRFPPLYVETGTWIGAVGVAAALALAVGLPPAWRARRLRIVDALAGHR"
     gene            complement(2896..>3492)
                     /locus_tag="VFX87_09695"
     CDS             complement(2896..>3492)
                     /locus_tag="VFX87_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995232.1"
                     /translation="IDLDIPRGDFVALMGPSGSGKTTLLNLIGGLDSPTGGRITVDGA
                     RIDQLSSGELSRWRAANVGFVFQFYNLLPVLSAQKNVELPLLLTDLSAGERRRRAGIA
                     LALMGLEDRARHKPRELSGGQEQRVAIARAIVTDPVLLVCDEPTGDLDRGTADEVLGL
                     LQMLNREHGTTVVMVTHDPKAAGHARRQLHLDKGVLAD"
BASE COUNT          581 a         1225 c         1235 g          451 t
ORIGIN      
        1 cccgccgggc tcatggcgac catcgtggaa ttgggctggg tgccgcccga gaaagccgtc
       61 cgcttcggca agggcggcaa ggtgcagccg gccggaccgc agatcaccat cacccagacc
      121 cacgccgagc attcctccga agtgaccgtg accgacccgg cgacgaagaa gaccacgacc
      181 taccccggcg gcgagcccgc gggcttcatc gtggagatgg agaacggctt caagctctac
      241 cacatgggtg acacggggct cttcggggac atgcgcctca tcgccgagta ctacaagccc
      301 gacctcatca tggtgcccat cggcgggcac ttcgtcatgg acccggcgga cgcggcgtac
      361 gcgacgaagg agatgctcaa gcccaagttc gccatcccgt tccactacgg caccttcccg
      421 gtgctcaagg gcacgccgca ggagtatcag gccgcgctcg gcgccgccac cacccaggtg
      481 ttccccatca gcccgggcga caagctgacg ttctagcgcc gagtcgacgc taggacgcgc
      541 gcaaggcgag ggtgacgggg aggcgcgccg cccggatcgc gggcggcacg ccacccaccg
      601 cgccgatggc cagcgcccag agcagcccct tccccaggag cgcccaggtc acgcggaaat
      661 cgaaggccac ctgggagaag gtgtcgaagt tgagcgtgga gagcgaggcg ccgtcgcagt
      721 acagataggc gagcccgccc ccgagcgcgc ccccgacggc ggacaggacc agcgcctcga
      781 tcagcaccga gacaaccacg ggggcgcttc cgaagccgat cgcgcggagg gtggcgattt
      841 ccacctggcg gctggcgatg gccgagtaca tgcagttgag cgccccgaag gtggcgccca
      901 cggccatgaa cgcggccacc gtatagccca gcacgttgat cagggtggcg agcaccttgg
      961 actgcttggc gtagtactcc ggctcccgcc acacgcggac ggagaagcgg ggatcgcgct
     1021 tgaccgcggt ggtgagtccg gccaggccgg cgtccgaacc atcgacgagc cgcgcggtga
     1081 gggactggaa cccctgccgg cggaacgccg acatggccac ctcggcatcc gcccagatct
     1141 cggactcgtg cacgtcgccc ccgctcgaga agatgccgac cacggtccag tcgctgtcgc
     1201 ggaaggcgat gcgcgagccg acgcccagcc cctcgaactg ctgcgccgcc cgccggccca
     1261 cgatgacttc gcgaaccccc cgcgcgaagc ggcggccctc caccagagcc agctcgtccc
     1321 gaatggcgaa ggcggccggc tgcacgccgc ggaacggcac attgttgggg tcggtctggc
     1381 cccggcgcgg caagtccacc atcaccacca gctcggcgga ggccagcggc cgcccctccc
     1441 cgtcgcgcgc gatggcgggc aggctcgcca gcagcacggt ctggtcgcgg gccacgccgc
     1501 tggacatctc ggcgtcggac ccggcacgca gcatgatcac gcgatcctcg cgccccgtgc
     1561 tcgccagcgt gtggcggaac ccctcggcca tggagagcac ggacacgagg acgcccgcca
     1621 cccccgcgat accgatgacg acgaccaggg aggtgccgag gcgctggggg agattgcgca
     1681 ggttgaccag cgtgatctgc gcgatctgtc ggagcgcgcg catcagcgat gccccgcgag
     1741 ggcgtccacg atgcgcaggc gccgagcgcg ccaggccggc ggcaggccca ccgccagcgc
     1801 cagggccgcg gccaccccca ccgccccgat ccaggtgccg gtctccacgt agagcggcgg
     1861 aaagcggccg ccggtggagt cgttcaggaa cgggagcatg ccgacggcgg cggccatgcc
     1921 cgccgcgccc ccgatgagca gcagcagcgc ggattccgcc agcaccagca cggtgacgcg
     1981 cgcgtccgag aagcccaggg tcttgaggat ggccagctcg gggatgcgct cccggaggga
     2041 ctgcgccatg gtattgccgg tgaggatgag gatggtgaag aacaccgcgg cgaggatgcg
     2101 cgagatcagc gcgccgatat cgccgatctg cttggcgaag ccgatggcga actccttctc
     2161 gggttgggtc ttggtctcgt ccggcgagtt cgcgaagagc cggtcgatgc ccgccgcgat
     2221 ctccttcgcc tggcccgact cggcgatgcg aatgatgtac cagccggtgc gcccccgcgc
     2281 gttctggcgg gcctcgtcga aatgcgcgac gttgatgagg accatgtcgg tattgccctg
     2341 gacggcggga tcctcggcgt cgatcacgcc gaccaggtcg aattcccagt tcatgtcgcc
     2401 cccggcgcgg ggatggatct ccgaggcgat gggaagcttc tggccgacct tccagccgaa
     2461 gcgctcgacg aggcggcggc ccgcgatggc gccggtgcgg gtcttggcga aggcctcgcg
     2521 ctccgcggcg gcgacggtga actccgggta catgtccagg taccgggacg gatccaccgc
     2581 gaacaccggg aaggcattgc tctcgttctg gtacttggcg ccgaaccact ccgcgtgcgc
     2641 cgcccccacc acgcccggca cgccttcgat acggcgcagg taggagatgg gcaggagctc
     2701 gatgatggag tagcgcgcgg tggtgagcag gcgcttggcg tcggcggagt cggccccgga
     2761 ctcgaaggcc gtcgtcaccg cctggagcag cccgaagagc atgaaggcca ccgtcaccga
     2821 gaggagcgtc agcaccgtgc gcgtcttctt gcggaagagc ccggcgagga gcagcggcac
     2881 gaagcgcatg gccgatcagt ccgccaggac gcccttgtcc aggtggagct ggcgccgcgc
     2941 gtggccggcg gccttgggat cgtgggtgac catcaccacc gtggtgccgt gctcgcgatt
     3001 gagcatctgg agcaggccca gcacctcgtc cgcggtcccc cggtcgaggt cgccggtggg
     3061 ctcgtcgcag accaggagca ccgggtcggt gacgatggcg cgggcgatgg ccacccgctg
     3121 ctcctgcccg ccggacagct cccggggctt gtggcgcgcc cggtcctcga gccccatcag
     3181 cgccagcgcg atcccggccc gccgccgccg ctcccccgcg gagagatcgg tgagcaggag
     3241 cggcagctcc acgttcttct gcgcggagag caccgggagg agattgtaga actggaacac
     3301 gaagccgaca ttggcggccc gccagcgcga gagctcaccg ctcgagagct gatcgatgcg
     3361 ggcgccgtcc accgtgatcc ggccgccggt gggcgagtcc agcccgccga tgaggttgag
     3421 cagggtcgtc ttgccggagc cggacggccc catgagggcc acgaaatcgc cgcgcgggat
     3481 gtcgagatcg at
//
LOCUS       DASUFA010000262         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1306032, whole genome shotgun sequence.
ACCESSION   DASUFA010000262 DASUFA010000000
VERSION     DASUFA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306032"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VFX87_09700"
     CDS             <1..1389
                     /locus_tag="VFX87_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4995233.1"
                     /translation="TKILVVDDEAEIRSLLDAVLQSKGFEVVTAADGAAALEQVQREH
                     PSVILMDLSMPRMNGMDALPEIKRLDPDVPVIICTAHADLATAVRAMKLGAYDYLTKP
                     FDVERLTLTLERAVERNQLRSRIDELKRQGQGSSLSERMGGSPAIAQVIQQVGQVAES
                     NFTVLVQGETGTGKELVARAIHQQSPRRQAPFVAVDCGAIPETLVESELFGHERGAFT
                     GAQTKREGHFQLAKGGTLFLDEIGNIPVATQAKLLRALEQREVQPLGSARAVAVDVRI
                     IAATNSELEEGVKVGRFRADLFYRLGEFTITLPPLRSRREDIAHLAQRFLDEVSMELR
                     RPVRRIADEAMQALLRHDWPGNVRELKNVIRKAALVATDEVTVEQLPALAVGAPAGPP
                     PVSAHPVGDLSLREVADLAAVQAERELIRHALRITKGNKSQAARLLRTDYTTLHAKMK
                     RYSISAREFMPT"
     gene            1729..1938
                     /locus_tag="VFX87_09705"
     CDS             1729..1938
                     /locus_tag="VFX87_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4995234.1"
                     /translation="MEARSRVGTCSLRQIVGGAVADAEGEAIRRALTATVGNKSRAAR
                     LLQTNYTTLHAKMKRYGISATEFQG"
     gene            complement(1952..>2360)
                     /locus_tag="VFX87_09710"
     CDS             complement(1952..>2360)
                     /locus_tag="VFX87_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX4995235.1"
                     /translation="KDAGSPFRSLTEPERAVFQGIIDEMYGRFVGLIAQARRMPEDRV
                     RGFADGRVYTADQALKLGLVDRVGYLEDVVALAKERAGLEEAKVIMYHRPKEYRTNIY
                     SLTPPPSTAESTLAQFAATLGGGGPRFLYLWWP"
BASE COUNT          376 a          833 c          822 g          329 t
ORIGIN      
        1 accaagattc tggtggtgga cgacgaggcg gaaattcggt cgctcctcga cgcagttctg
       61 cagtccaagg gattcgaggt cgtcaccgcg gcggacggcg ccgccgcgct cgagcaggtc
      121 cagcgcgagc accccagcgt gatcctgatg gatctcagca tgccgcgcat gaacggcatg
      181 gacgcgctgc cggagatcaa acgcctggac ccggacgtgc cggtcatcat ctgcaccgcc
      241 cacgccgacc tggccaccgc ggtgcgggcc atgaagctcg gggcctacga ctacctcacc
      301 aagccgttcg acgtggagcg gctgaccctg acgctcgagc gcgcggtgga gcgcaaccag
      361 ctccgctcgc gcatcgatga gctgaagcgc cagggacagg ggagctcgct gagcgagcgc
      421 atgggcggca gccccgcgat cgcccaggtg atccagcagg tgggccaggt cgccgaatcc
      481 aacttcacgg tgctggtgca gggcgagacc gggaccggca aggagctggt ggcgcgcgcc
      541 attcaccagc agagcccgcg ccgacaggct ccgttcgtgg cggtggactg cggcgcgatc
      601 ccggagacgc tggtggagtc ggagctcttc ggccacgagc gtggggcgtt caccggcgcc
      661 cagaccaagc gggagggcca ctttcagctc gcgaagggcg gcaccctgtt cctcgacgag
      721 atcggcaaca tcccggtggc gacccaggcg aagctgctcc gggcgctgga gcagcgggaa
      781 gtccagccgc tcggcagcgc ccgcgcggtg gcggtcgacg tgcgcatcat cgcggccacc
      841 aactcggagc tcgaggaagg cgtgaaggtc ggacggttcc gggccgatct cttctaccgg
      901 ctcggcgagt tcacgatcac gctgccgccg ctgcgcagcc gacgcgagga catcgcccat
      961 ctcgcccagc gctttctcga cgaggtgagc atggagctgc ggcggccggt gcgccggatc
     1021 gcggacgagg ccatgcaggc gctgctccgg cacgactggc ccggcaacgt gcgcgagctg
     1081 aagaacgtga tccgcaaggc cgcgctcgtg gcgaccgacg aggtcacggt ggagcagctc
     1141 cccgcgctcg ccgtgggggc ccccgccggg ccgccgcccg tctccgcgca ccccgtgggc
     1201 gatctctcgc tgcgcgaggt cgcggacctg gccgcggtgc aggccgagcg cgagctgatc
     1261 cggcacgcgc tccggatcac gaagggcaac aagagccagg cggcccgtct cctacgcacc
     1321 gactacacga cgcttcacgc caagatgaag cgctactcga tctccgctcg cgagttcatg
     1381 cccacttgat gcggctctgg cggcaccgct atggtctgcg cgccatggtt ctcgatcctc
     1441 cgcgataccc cgggctcggg gggggccgcg ccggtctgcg tgcgcactcc tccaagtagc
     1501 tggaaaggct gatcggactc cccgccgcac ccgtcccctc cgcgtggcac cgcccttgcc
     1561 gtgagacgag acaggtagtc gcatcagcga attccgcagg aggcacacat gagtgccgtc
     1621 gaagacgtat tgccctggat ctcgagcctg gacatggggc cgctctcagg ggtttcgatg
     1681 accggctccc tggccggccg agcggtccag cgcccgacca ctcggacggt ggaagcccgt
     1741 tcgcgagtcg gcacctgctc gctccgccag atcgtgggcg gcgcggtggc cgacgcggag
     1801 ggcgaggcga tccgccgcgc gctcaccgcc acggtcggca acaagagccg ggccgcgcgc
     1861 ctgctccaga ccaactacac gacgctgcac gcgaagatga agcgctacgg catctccgcc
     1921 acggagtttc agggctaggg ccgtcagatc ctcacggcca ccagagatag aggaagcggg
     1981 gcccgccgcc gccgagggtg gccgcgaact gggccagggt gctctccgcg gtggacgggg
     2041 gcggcgtcag cgagtagatg ttcgtgcggt actccttggg gcggtggtac atgatgacct
     2101 tcgcctcctc gagcccggcc cgctccttcg ccagcgccac cacgtcctcg agatagccca
     2161 cgcgatccac cagccccagc ttgagcgcct ggtcggccgt gtagacgcgg ccgtcggcga
     2221 acccgcgcac ccggtcctcc ggcatccggc gcgcctgggc gatcagcccc acgaagcgcc
     2281 cgtacatctc gtcgatgatg ccctggaaca ccgcgcgctc cggctcggtg agggagcgga
     2341 atggcgagcc ggcgtccttc
//
LOCUS       DASUFA010000263         5522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1558139, whole genome shotgun sequence.
ACCESSION   DASUFA010000263 DASUFA010000000
VERSION     DASUFA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558139"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2064
                     /gene="fusA"
                     /locus_tag="VFX87_09715"
     CDS             1..2064
                     /gene="fusA"
                     /locus_tag="VFX87_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX4995236.1"
                     /translation="MAKIRNVAFVGHGGVGKTSLVEAILFACGASTRLGRVDDGTTTT
                     DFDPDEIKRKISLNTAVGFADHQGHRLNLIDTPGYGDFVADARAGLRVAEAAVVVVDA
                     VAGVQVQTEKVWKFANDFALPRAIVVNRLDRERADFFRTLESLQKRLKGRLVPMQLPI
                     GAENSFTGVVNLIAMRALVLADGKIKEADIPADLVETARSYREKLTEAAAETDDDLLA
                     KYLEEGAIGEAEMLAALRKAIMSGDLVPILASSATRGIGVGPLIDLIVKEFPSPADAA
                     AVEGTDPRTKAAARRAADPKAPLSAVVFKTISDPHVGKLSVFRVYSGTLRADSQVFNA
                     SRDSRERIGHLGWLSGKTQKPVEALGPGEIGVVAKLKDTLTGDTLSDEAHPVVLPGIS
                     FPEPAISFAIQPKTRGDEDKISTALHRMAEEDPTLHHHYDPETKQLLVSGMGQLHVEI
                     VVERMKRKYNVDVSLLPPRIPYRETVKGRAEVQGKYKKQTGGRGQYGDTWLRVEPLAR
                     GGGFEFVDDIFGGAIPRNFIPSVEKGVRDCMKRGILAGYPVVDLKVTLYDGSYHDVDS
                     SDMAFQIAASMGLQKGFMEAKPCLLEPVMNVEVTAPSEGAGDVIGDLNGRRGRIVGME
                     PDGDVVAVRAQVPMSEMLTYESSLRSMTGGRGGYSMEFSHYEEVPSQLAEKVVAAHRA
                     EKEKH"
     gene            2077..3309
                     /locus_tag="VFX87_09720"
     CDS             2077..3309
                     /locus_tag="VFX87_09720"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX4995237.1"
                     /translation="MFVTHIECTVCGHHHPAGGVLTVCARCGQMLAVRYDLPRVAAAV
                     TKEALRPRPPGMYRFRELLPLAADEEPVTLGEGGTPLLALPRLAAHLGLRHLLAKDEG
                     QNPTGTFKARGLGMAISKARTLGVRGLMIPSAGNAGGAAAVYGARAGIPVAVVVPRDT
                     PEAAIAEAALAGAHVFTVDGTIATAGRAIAAVAPEIGWFDLATLKEPYRLEGKKTMGL
                     ELAEQLGWDAPDVLVYPTGGGTGLVGIWKAYEELAGMGWVKAGQPRFVAVQAEGCAPL
                     ARAWDEKADTTSTVEHPFTNAPGLRVPGPFAGRQMLRLLRETGGFPRTVGELAIVEAQ
                     RLLARVEGIWTAPEAAATLAALLQMKDEGLVTSTERIVLILTGAGIKNPPPPLAAPVH
                     LEGTPAQMLARVKEAIRP"
     gene            3497..4675
                     /locus_tag="VFX87_09725"
     CDS             3497..4675
                     /locus_tag="VFX87_09725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX4995238.1"
                     /translation="MTGKGDFAPKKGPRVVVIGGGWGGATAAKYLRLLDPNIEVVLIE
                     PNREFVSCPFSNLVLAGLRSIDSLTMGYDGLRKHGVRIIHETATAIEPDQKRVRLGEG
                     YLQYDRLVVSPGVDFQWEQVEGLAQNQDKVLHAWKAGPQTVQLARQLAAMPDGGVFVL
                     SIPLAAYRCPPGPYERTSMVAWYLKTNKPRSKLIVLDANQNIISKTALFRAAWQAYPN
                     IDYRPASRVIAVDPGSREVRTEFDKIRYDVVNVIPPQRAGAIAVQADLVGGDKRWCEV
                     NHVTYESVKQPGIHVIGDATIGLPVPKSGNVANAMGKITAASVVSLINGKQPPALAPG
                     NTCYSWVSDREAIAVVNAYRIENGKVVQIEQKLTPAQSPLVAQRAIGWTQSIWADILA
                     "
     gene            4705..5310
                     /locus_tag="VFX87_09730"
     CDS             4705..5310
                     /locus_tag="VFX87_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4995239.1"
                     /translation="MRLTPRWIALVVALALVALAGAARAAGPLDERGALKSVTCAACH
                     GQAGNSRSSAMPIIAGQDAAYLKKQIEDYAAGKRLSAEMEPYAKQALNLGVDDVAAYF
                     ASRRREPTPVQSPTDAVARGRSAAAQCVICHGPQGQGDAAKLIPSLAGQAPGYLREQM
                     LLFKQDRRSPGDPALTAVKALLKTIPDETLGDLAAYYSSLR"
BASE COUNT          863 a         1930 c         1952 g          777 t
ORIGIN      
        1 atcgccaaga tccgcaatgt cgcattcgtc ggccacggcg gcgtgggcaa gacctccctg
       61 gtcgaggcga tcctgtttgc atgcggagcc agcacccggc tcgggcgtgt ggacgacgga
      121 accacgacca ccgacttcga ccccgacgag atcaagcgca agatctcgct gaataccgcg
      181 gtcggcttcg ccgatcacca gggccaccgg ctcaacctca tcgacacccc cgggtacggc
      241 gacttcgtgg ccgacgcccg cgcgggcctg cgcgtggcgg aggccgcggt ggtggtggtg
      301 gacgcggtgg cgggcgtgca ggtgcagacc gagaaggtct ggaagttcgc caacgacttc
      361 gcgctgccgc gcgcgatcgt ggtgaaccgg ctcgaccgcg agcgggccga cttcttccgc
      421 acgctcgagt cgctccagaa gcggctgaag ggccggctcg tcccaatgca gctcccgatc
      481 ggcgccgaga acagcttcac tggggtggtg aacctgatcg cgatgcgggc gctcgtgctg
      541 gccgacggca agatcaagga ggccgacatc ccggccgatc tcgtcgagac cgccagatcc
      601 taccgcgaga agctgaccga ggccgccgcc gaaaccgacg acgatctcct cgcgaagtac
      661 ctcgaggagg gtgcgatcgg cgaggccgag atgctggccg cgctgcgcaa ggcgatcatg
      721 tccggcgatc tcgtgccgat cctggccagc tcggcgacgc gcggcatcgg ggtggggccg
      781 ctgatcgacc tcatcgtgaa ggaattcccg tcgcccgccg acgccgcggc ggtggagggc
      841 acggacccgc gcaccaaggc cgcggcccgc cgcgccgccg atcccaaggc gccgctcagc
      901 gcggtggtgt tcaagacgat ctcggacccg cacgtgggca agctctccgt cttccgggtc
      961 tactcgggga cgctgcgggc cgacagccag gtcttcaacg cgagccgcga cagccgcgag
     1021 cggatcgggc acctcggctg gctctccggc aagacgcaga agccggtcga ggcgctcggc
     1081 ccgggcgaga tcggggtggt cgccaagctc aaggacacgc tgaccggcga cacgctctcc
     1141 gacgaggcgc atccggtcgt gctgcccggc atctcctttc cggagcccgc gatctccttc
     1201 gcgatccagc ccaagacccg cggcgacgag gacaagatct cgaccgcgct gcaccgcatg
     1261 gccgaggaag accccacgct gcaccatcac tacgacccgg agaccaagca gctgctggtg
     1321 tcgggcatgg gacagctcca cgtcgagatc gtggtcgagc ggatgaagcg gaaatacaac
     1381 gtcgacgtct cgttgctgcc gccgcgcatt ccctaccggg agacggtcaa gggccgggcc
     1441 gaggtgcagg gcaagtacaa gaagcagacg ggtgggcgcg gccagtacgg cgacacgtgg
     1501 ctgcgcgtcg agccgctcgc ccgcggcggc ggcttcgagt tcgtggacga catcttcggc
     1561 ggcgccatcc cgcgcaactt catcccctcg gtcgagaagg gcgtgcgtga ctgcatgaag
     1621 cgcggcatcc tcgcgggcta cccggtggtg gatctgaaag tcacgctcta cgacggctcc
     1681 tatcacgacg tggactcctc ggacatggcg ttccagatcg ccgcgtcgat gggcctgcag
     1741 aagggcttca tggaggccaa gccctgcctg ctcgagccgg tcatgaacgt cgaggtcacc
     1801 gcgccgtcgg agggcgcggg cgacgtcatc ggcgacctca acggccggcg gggccgcatc
     1861 gtgggcatgg agccggacgg cgacgtggtg gcggtgcgcg cgcaggtgcc gatgtccgag
     1921 atgctcacgt acgagtcgag cctccgctcg atgaccggcg ggcgcggcgg ctactcgatg
     1981 gagttctccc actacgagga agtgccgagc cagctcgccg agaaggtcgt cgccgcccac
     2041 cgcgccgaga aggagaagca ctagattcgt ccgcacatgt tcgtcaccca catcgaatgc
     2101 acggtctgcg gtcatcatca tcccgccggc ggggtcctca cggtgtgcgc tcggtgcggg
     2161 cagatgctcg cggtgcgcta cgatctgccc cgcgtcgcgg ccgcggtcac caaggaggcc
     2221 ctccggcccc gtccgcccgg gatgtaccga ttccgcgagc tgctcccgct cgccgccgac
     2281 gaggagccgg tgaccctggg cgaggggggc acgccgctgc tcgcgctgcc gcggctcgcc
     2341 gcgcacctgg gcctgcgtca cctcctggcc aaggacgagg gccagaaccc gaccggcacc
     2401 ttcaaggcgc gcggcctcgg catggcgatc agcaaggccc gcacgctcgg cgtgcgcggg
     2461 ctcatgatcc cgtcggcggg gaacgcgggc ggcgcggccg cggtctacgg ggcccgcgcg
     2521 ggcatcccgg tggcggtggt ggtgccacgc gacacgcccg aggccgccat cgccgaggcc
     2581 gccctggccg gcgcccacgt gttcacggtg gacggcacca tcgccaccgc ggggcgcgcc
     2641 atcgcggcgg tggcgcccga gatcggctgg ttcgacctgg ccacgctcaa ggagccctac
     2701 cggctcgagg gcaagaagac catggggctc gagctggccg agcagctcgg ctgggacgcg
     2761 ccggacgtgc tcgtgtatcc caccggcggc ggcaccggcc tggtgggcat ctggaaggcg
     2821 tacgaggagc tggccggcat gggctgggtg aaggccggcc agccgcgctt cgtcgcggtc
     2881 caggccgagg gctgcgcccc gctcgcgcgc gcctgggacg agaaggccga caccacgtcc
     2941 acggtggagc acccattcac caacgccccg ggcctgcgcg tcccggggcc cttcgcgggc
     3001 cggcagatgc tccggctcct tcgcgagacc ggcggcttcc cgcggacagt cggcgagctc
     3061 gccatcgtgg aggcccagcg actcctggcc cgggtggagg ggatctggac cgcgcccgag
     3121 gcggcggcca cgttggccgc actccttcag atgaaggatg aaggcctggt gacgtcgacc
     3181 gagcgcatcg tcctgatcct cacgggggca gggatcaaga acccgccgcc gccgctcgcg
     3241 gcgccggtgc acctcgaggg cacgccggcc cagatgctcg cccgggtgaa agaagcgatc
     3301 cgcccgtaat tctccttgac acggcgtcgg gccgatttgt agattctcga cactcccggt
     3361 cgagaatcgg gagaattgcc ggctcgctgc ggaggggcat actttgagca cggtcagtcg
     3421 cagagggttc ctacgggcgt cgggactcgg cctgggtgtg ctggccggcg gcggcctcgc
     3481 cgggtgcgcc gcgcccatga cgggtaaggg ggacttcgcg cccaagaagg ggccgcgggt
     3541 cgtcgtcatc ggcggcggct ggggcggagc gacggcggcc aagtacctgc gcctgctcga
     3601 cccgaacatc gaggtggttc tgatcgagcc caaccgcgag ttcgtctcct gcccgttcag
     3661 caacctggtc ctggcgggcc tgcgctcgat cgattcactc accatgggct acgacggcct
     3721 gcgcaagcat ggggtgcgga tcatccacga gaccgccacc gccatcgagc ccgatcagaa
     3781 gcgcgtgcgg ctcggcgagg gatatctgca gtacgaccgc ctcgtggtct ctcccggcgt
     3841 ggacttccag tgggagcagg tggaagggct cgcccagaac caggacaagg tcctgcacgc
     3901 gtggaaggcg gggccgcaga ccgtgcagct cgcccggcag ctcgcggcga tgcccgacgg
     3961 cggtgtcttc gtgctgagca tcccgctcgc ggcctaccgg tgcccgcccg gcccctacga
     4021 gcgcacctcg atggtggctt ggtacctcaa gaccaacaag ccccggtcca agctgatcgt
     4081 gctggacgcc aaccagaaca tcatctcgaa gaccgcgctc ttccgcgcgg cctggcaggc
     4141 ctatccgaac atcgactatc ggcccgcgag ccgcgtcatc gcggtcgatc cgggcagccg
     4201 ggaagtccgc accgagttcg acaagatccg ctacgacgtg gtcaacgtga tcccgcccca
     4261 gcgcgcgggc gccatcgcgg tgcaggcgga cctggtcggc ggcgacaagc gctggtgcga
     4321 ggtcaaccac gtcacctacg agtcggtgaa gcagccgggc atccacgtga tcggcgacgc
     4381 caccatcggc ctgcccgtgc ccaagtcggg caacgtggcc aatgcgatgg gcaagatcac
     4441 cgccgcctcg gtggtcagcc tgatcaacgg caagcagccg cccgcgctcg cgcccggcaa
     4501 tacctgctac agctgggtga gcgaccgaga ggccatcgcg gtggtgaacg cctaccggat
     4561 cgagaacggc aaggtcgtgc agatcgagca gaagctcacg ccggcgcaga gcccgctggt
     4621 ggcccagcgg gccatcggct ggacccagtc catctgggcg gacatcctgg catgagcgcc
     4681 acgtgtccgg ccatgcgtct gatcatgcgt ctgacgccac gctggatcgc cctggtcgtc
     4741 gcgctcgcgc tggtcgcgct ggccggtgcc gctcgcgcgg cgggcccgct cgacgagcgg
     4801 ggcgcgctca agtccgtcac ctgcgcggcc tgccacgggc aggcgggcaa tagccgctcg
     4861 agcgcgatgc cgatcatcgc gggccaggac gcggcctacc tgaagaagca gatcgaggac
     4921 tacgcggccg ggaagcggct ctcggccgag atggagccct acgccaagca ggcgctgaat
     4981 ctgggcgtgg acgacgtcgc ggcgtacttc gcctcccgcc ggcgcgagcc gacgcccgtc
     5041 cagtcgccga ccgacgcggt ggcccgcggg cggagcgcgg cggcccagtg cgtgatctgt
     5101 cacggcccgc agggccaggg cgacgcggcc aagctgattc cgagcctggc cggccaggcc
     5161 cccggctatc tgcgcgagca gatgctgctg ttcaagcagg atcggcgcag cccgggcgat
     5221 ccggcgctga ccgcggtcaa ggcgctgctg aagaccatcc ccgacgagac cctgggcgac
     5281 ctggccgcgt actactcgag cctccgctaa gccggcgctc gaacgacggc agtcgcgcta
     5341 tcatgggggc cttcatgagc caccatgccc tgcgccaggc gcgcgcgtga cgccgacgtt
     5401 cgagatcgcg ctcttcgtcc tcaccttcgt cggagccttc gtctccggtc tcgtcggcgt
     5461 gggcggtgcc atcgtgatga tcccgctgct ctactacgtc ccgccgctgc tgggcgcggg
     5521 tg
//
LOCUS       DASUFA010000264         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1437166, whole genome shotgun sequence.
ACCESSION   DASUFA010000264 DASUFA010000000
VERSION     DASUFA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437166"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFX87_09735"
     CDS             <1..208
                     /locus_tag="VFX87_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995240.1"
                     /translation="EVVPPAHGRVLFDRAHDPKRLHVIADADHRLSEPAHRREAVEES
                     RRWLLAHLPHAAPAGRPAPTGRIR"
     gene            205..1350
                     /locus_tag="VFX87_09740"
     CDS             205..1350
                     /locus_tag="VFX87_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4995241.1"
                     /translation="MINAARLKDEFLELVSISSPSKREGTIARRLEAILKGMGASVEV
                     DGAGEKIGGNTGNLLARFPGNTPGATPFLLSGHMDTVGPAEAIHPVVEGDVIRTDGTS
                     VLGGDDKAGVVAILEAVRVLRERRIPHGDLEVVLTICEEYGLLGAKNFDTGRLRAKRG
                     LVLDVDGVCELITRAPAANRMTFTVHGLAAHAGICPEQGMSAIRIASEAIAAMKLGRL
                     DAETTANLGIIQGGLAGNIVPASVTVRGETRSLSLERLAAQTEHMRRCFDEAAARHHV
                     QVGDRQYQARVETQIDHQYGRLDIRDEAAIVRLVQSAAGAMGRACATRGTGGGSDANV
                     FVERGLEIANLACGMREIHTVNEWVDVRDLVLTAGLVVETVRLNAAA"
     gene            1394..2182
                     /locus_tag="VFX87_09745"
     CDS             1394..2182
                     /locus_tag="VFX87_09745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995242.1"
                     /translation="MTEHIDDLSLEHAAPAGRPRSRSVLFVHGMWGGSWYFRNYLYAA
                     AQAGWDAWALNLRGHGSSPAPGGLGRVSLADYVADVRRCLDHLGEAVLVGHSMGGLIA
                     QKAAEGGGVAAAAFLTSAPPRGINAIAWPVLSRMARYVPAMLGGRAFTASRAHADFLF
                     LNGLTPEQRDWAFPRFGAESGRAARELALGGLAVDARAIRCPTLVVGADQDRITPAAL
                     QRRIAARYRAEYQEAAGHAHMLMLEEGWERPFKDMLAWMERGTR"
     gene            2179..3171
                     /locus_tag="VFX87_09750"
     CDS             2179..3171
                     /locus_tag="VFX87_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4995243.1"
                     /translation="MNASLDLESSPPDRPTILIVEDDQHIRDALSGLLGTLGYRLLMA
                     ASAEQALDALNVVSPDLVLTDVHLGAMSGIELCARLKADPRYELMPVVILTAVGDLEG
                     RVAGLAAGADDFFTKPVAFVELRTRLAALLRVRLLLSQLERAEAVITTLALTIEARDP
                     YTLGHCDRLSRYAVAVGEALGLDPEMVRSLRLGGYLHDLGKISVPDGILLKPGPLDPI
                     EQERIRAHPGAGSDLVLGLRSMELVRPIMRHHHERWDGSGYPDGLKGEAIPLGARIIS
                     VVDVFDALHTDRPYKAALPRSEAVSTLIRETDAGYWDPRVVEAFLEILRHFKHD"
     gene            complement(3309..3455)
                     /locus_tag="VFX87_09755"
     CDS             complement(3309..3455)
                     /locus_tag="VFX87_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX4995244.1"
                     /translation="MLALGTLVSAVTAFVAVRWLLRWIQTHSFVSFGWYRVALGLVLL
                     TRLG"
BASE COUNT          510 a         1247 c         1232 g          479 t
ORIGIN      
        1 cgaggtggtg ccgcccgcgc atgggcgcgt cctcttcgat cgcgcccacg acccgaagcg
       61 cctgcacgtg atcgcggacg ccgatcaccg gctctccgag cccgcgcacc gccgcgaagc
      121 ggtcgaggag agccggcgct ggctgctcgc ccatctgccc cacgcggccc ccgcgggccg
      181 acccgcaccg acaggaagga tccgatgatc aacgcggcga gactcaagga cgagtttctg
      241 gagctggtgt ccatctcgag cccctccaag cgcgagggca cgatcgcgcg gcggctcgag
      301 gccattctga agggcatggg cgcgagcgtc gaggtggacg gggccggcga gaagatcggc
      361 ggcaatacgg gcaacctgct cgcgcgcttc cccggcaaca cgccgggcgc cacgcccttc
      421 ctgctctcgg gccacatgga cacggtggga cccgccgagg ccatccaccc ggtggtcgag
      481 ggcgacgtga tccgcaccga cggcaccagc gtgctgggcg gtgacgacaa ggcgggcgtg
      541 gtcgcgatcc tggaggcggt ccgcgtcctc cgcgagcgcc gcatcccgca cggcgatctc
      601 gaggtggtgc tcacgatctg cgaggaatac gggctgctgg gcgccaagaa cttcgacacc
      661 ggccgcctcc gcgcgaagcg cggactggtg ctggacgtgg acggcgtctg cgagctgatc
      721 acgcgcgcgc ccgcggccaa ccgcatgacg ttcaccgtcc acggcctggc cgcccacgcg
      781 ggcatctgcc cggagcaggg gatgagcgcg atccgcatcg cctcggaggc gatcgcggcg
      841 atgaagctcg gccgcctgga cgcggagacc accgcgaacc tcggcatcat ccagggcggg
      901 ctcgccggca atatcgtgcc cgcctccgtg acggtgcgag gcgagacgcg cagcctgagc
      961 ctggagaggc tcgcggccca gaccgagcac atgcgacgct gcttcgacga ggcggcggcc
     1021 cggcatcacg tgcaggtggg cgaccggcag tatcaggcgc gcgtggagac ccagatcgac
     1081 catcagtacg gccggctcga catccgcgac gaggccgcga tcgtccgcct ggtgcagtcc
     1141 gcggccggcg cgatggggcg ggcctgcgcc acgcgcggca ccgggggcgg ctccgacgcc
     1201 aatgtcttcg tggagcgcgg cctcgagatc gccaacctgg cctgcggcat gcgggagatc
     1261 cacaccgtga acgagtgggt ggacgtccgc gacctcgtgc tcaccgccgg gctcgtcgtg
     1321 gagacggttc gcctcaacgc cgcggcctga ccgggccgcc cggtcgcctt ccgcgtggcg
     1381 taagataacc accttgaccg agcacatcga cgacctgagc ctcgagcacg ccgcccccgc
     1441 cggacggccc cgctcccgct ccgtgctctt cgtccacggc atgtggggcg ggagctggta
     1501 cttccggaac tacctctacg cggccgcgca ggccggctgg gacgcgtggg cgctcaacct
     1561 ccgcgggcac ggctcgagcc cggccccggg cgggctcggg cgcgtgagcc tcgccgacta
     1621 cgtcgccgac gtgcggcgct gcctcgatca tctcggcgag gcggtgctcg tcggacactc
     1681 gatgggtggc ctgatcgcgc agaaggccgc cgagggcggc ggggtggccg cggcggcctt
     1741 tctgaccagc gcgccgccgc gcgggatcaa cgcgatcgcg tggccggtgc tctcgcgcat
     1801 ggcccgctac gtgccggcca tgctcggcgg ccgcgccttc acggcgagcc gtgcccacgc
     1861 cgacttcctg ttcctgaatg ggctcacgcc cgagcagcgg gactgggcct tcccacgctt
     1921 cggggcggag tcggggcggg ccgcgcgcga gctggccctg ggcggcctcg cggtggacgc
     1981 ccgcgcgatc cggtgtccca cgctggtagt aggtgccgat caggaccgca tcacgcccgc
     2041 cgcgctccag cgccggatcg ccgcgcgcta ccgtgcggaa taccaggaag cggcgggcca
     2101 cgcccacatg ctgatgctcg aggagggctg ggagcggccg ttcaaggaca tgctggcgtg
     2161 gatggagcgg ggcactagat gaacgctagc ctcgatctcg aatcctcgcc acccgaccgg
     2221 ccgacgatcc tgatcgtcga ggacgaccag cacatccgcg acgccctctc gggattgctc
     2281 gggacgctcg gctaccggct gctcatggcc gcctcggccg aacaggctct cgacgcgctg
     2341 aacgtggtga gcccggacct ggtgctcacc gacgtgcatc tgggcgccat gagcggcatc
     2401 gagctctgcg ccaggctcaa ggccgatccg cgctacgagc tgatgccggt ggtcatcctc
     2461 accgcggtgg gcgatctgga ggggcgggtg gccgggctcg ccgcgggggc cgacgacttc
     2521 ttcacgaagc cggtggcgtt cgtcgagctg cgcacccgcc tggccgcgct gctgcgggtg
     2581 cgcctgctgc tgagccagct cgagcgggcc gaggcggtca tcaccaccct ggcgctcacc
     2641 atcgaggcgc gcgatccgta caccctgggc cactgcgacc ggctgtcgcg ctacgcggtg
     2701 gcggtgggcg aggcgctcgg gctcgatccg gagatggtgc gctcgctacg cctgggcggc
     2761 tacctccacg acctcggcaa gatctcggta ccggacggca ttctgctgaa gcccggccct
     2821 ctcgatccca tcgagcagga gcggatccgc gcgcatccgg gtgccggctc cgacctggtc
     2881 ctgggcctgc gcagtatgga gctggtgcgc cccatcatgc gtcaccatca cgagaggtgg
     2941 gacggctcgg gctaccccga cgggctgaag ggcgaggcga tcccgctggg tgcccgcatc
     3001 atctcggtgg tggacgtctt cgacgcgcta cacacggacc gcccatacaa ggcggcgctc
     3061 ccgcgctcgg aagcggtctc cacgctgatc cgcgagaccg acgccggcta ctgggacccc
     3121 cgcgtcgtcg aggccttcct cgagatcctc cgccacttca agcacgactg acggggtcag
     3181 gtcttacatt ccgacatttg ctcgcgggag gtgtgccgtc tcgcggggga aatgtcggaa
     3241 tgtaagacct gacccctacc tgagtcacgt cgtgcgccgt cgcgtcgact agctccagtc
     3301 ggaccggctc agccgagccg ggtcagcagg acgagaccga gagccacccg gtaccaccca
     3361 aacgagacga agctgtgcgt ctggatccag cggagcagcc accgcaccgc cacgaaggcg
     3421 gtcaccgcgg agaccagggt gcccagcgcc agcagccccc agtcggtc
//
LOCUS       DASUFA010000265        11121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1306604, whole genome shotgun sequence.
ACCESSION   DASUFA010000265 DASUFA010000000
VERSION     DASUFA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306604"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFX87_09760"
     CDS             complement(<1..318)
                     /locus_tag="VFX87_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995245.1"
                     /translation="MSRRLKRWLIVLAALLVVGIAFIWTLPEIVKWQALKQIPAMTGR
                     AVAIEDIDINLFTGRVAIKKFRLAEADPGQTFVGLERLDVRFLWRSLFGNVRLAELRL
                     TEPI"
     gene            complement(358..1263)
                     /gene="mmsB"
                     /locus_tag="VFX87_09765"
     CDS             complement(358..1263)
                     /gene="mmsB"
                     /locus_tag="VFX87_09765"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX4995246.1"
                     /translation="MTRIGFIGVGTMGLPMAKNLVKKGFTVTAFDSNPEAVKAAAAAG
                     MTAAASAAEAVATADIVVTMLPSSPHVESVYTGDGGVLAAARKGTLCVDMSTIDPAVS
                     QRVAKAAQERGVRFMDAPVSGGTPRATDGTLAIMVGGAAADFEEAKPALAAMGTNVIH
                     VGAVGSGEVVKLCNNLIAGVCGVAVSEAFRIAEGFGMDPKVVTDVISKSSGNTFLMGF
                     MHPVPGITPTAASSNEYQPGFMTDLMCKDLGLAVEAARNLRVPLFVTPAAQQVYRLAS
                     SHGLGRRDFTSVYSFLKPSSGQAPV"
     gene            complement(1260..2168)
                     /locus_tag="VFX87_09770"
     CDS             complement(1260..2168)
                     /locus_tag="VFX87_09770"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995247.1"
                     /translation="MSEQIGFIGLGNMGVPMAGRLLDGGYTLVVHDIRQDAARPLLAR
                     GATWAASPAEVAAAAQTVITILPTSREVRAVLLGSKGLLDALRPGSLVLEMTSADPSV
                     TRELEREVAARGSALIDAPVSGGVRGAVEGTLAIMVGGDPALLERARPVLARMGKNIF
                     HAGPVGAGHAIKLVNNMCSGGILALTIEAVAVAARAGVDPTRAVEILQASSGRSNASD
                     NKFPRFILNGAFDAGFAIRLMMKDLDGYGRLAQEAGVPSPVARAAAEVYRVAMARGMA
                     EQDHTAIARIIEEWAGVSLRSQGGKA"
     gene            2202..3098
                     /locus_tag="VFX87_09775"
     CDS             2202..3098
                     /locus_tag="VFX87_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4995248.1"
                     /translation="MLLGLVLIAIAAASWGTTGATMTLLSRGAAASPLLVGWSRLAIA
                     APVLLLAAAGVGRFGARHPTLRPWPSLADLPLCAALGLAMAAYQVCYFRAVTLVGVAP
                     AALLAICSAPLLIAVLAALFLGERLTPIVRLSLGMAVVGTALLVVGPRGFGEIAGHFG
                     LGAVLALGAGVSYAVYAVAAKGLLARVTPLAVSAITFALAALFLAPALLGETAPARAL
                     AAGWPLLLYLGIGPTAVAYVMFTAGLGRVPATAAGIVSLLEPLTATTLGLLAFGERLG
                     PIGVAGAVLLLAALALLTTARR"
     gene            complement(3203..4252)
                     /locus_tag="VFX87_09780"
     CDS             complement(3203..4252)
                     /locus_tag="VFX87_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995249.1"
                     /translation="MSAAVEATLTLPEEDVYAEFCKREWCDGLPFVPPTAERVRAMLA
                     GAKTKAAESLGVMPPLWRECAVEKLAVNAVMAGCEPAYFPVIVAAAQALLDPAFNLYG
                     VQATTHPVAPLVVVTGPYARTIGLHSGSGLFGPGFRANATIGRAIRLILMNVGGGWPG
                     RGDMATQGSPAKFSYAITEREDSPWEPLHVALGFKADQSVVTVFGGEGPHNVNDHVST
                     TAPGILNNVADVATTLGSNVGWYLSQSQILVVLGPEHAATIAADGFTRADVQRFVFEN
                     ARMPLGRMKLGGMWGMHDWPTWMAKETDESVRLPRVPAAEDVYVMVGGGSGKHSCVVP
                     NCTFSRAVSRVLDRI"
     gene            complement(4260..4436)
                     /locus_tag="VFX87_09785"
     CDS             complement(4260..4436)
                     /locus_tag="VFX87_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995250.1"
                     /translation="MITTTVFQRLALGEMVALGAAGLPLLIVQHPLGGEASEGVSRRI
                     AQAVEQLGAMLGAR"
     gene            complement(4503..4790)
                     /locus_tag="VFX87_09790"
     CDS             complement(4503..4790)
                     /locus_tag="VFX87_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995251.1"
                     /translation="MPLITVLSPVGELRTATTAIPPLPADLRGLTVGFLDNTKHNFDR
                     LVTGIGEVLKERHGVKRVVILRKANAATGAAHSIIEELSKECDLVFAGSAD"
     gene            4867..6336
                     /locus_tag="VFX87_09795"
     CDS             4867..6336
                     /locus_tag="VFX87_09795"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX4995252.1"
                     /translation="MLVLALDVGTSSARARVFDEAGRAQPGAEGQVTYEPRTTPDGGV
                     ELDADALLAAVTSALDTCLAGCGARAAEIAAVGASVFWHSLLALDGAGRPLTPVITWA
                     DTRSAPAAAALRRAHDERRIHARTGAPVHPAFFPAKLRWLHETRPDVFARAAIWCGFG
                     EYLHARLTGRLATSLSMASGTGLLDQIGGGWDGDLLAASGIAAAHLPPIDDTAAPGLI
                     APHAARWPALARVAWHPARGDGACSNVGSDCWGPGRVALNVGTSAALRVVLPEPLGPH
                     GTTPWGLWRYRVDARRALVGGATSEGGNVLAWCRRHLALPDDPAALEAALGAAEPDGH
                     GLTALPFLAGERSPGWRPEARAAVTGLSLDTGALDITRALLESVAFRLAEVYDRLRPL
                     AAADHVVVGSGGALAHSPAWAQIVTDALGVPVALGGDAEASARGAALLALEALGVAVP
                     GAPPPLRVLQPAPERHARYRAARERQRLAYDNIVGFNHS"
     gene            6358..8673
                     /locus_tag="VFX87_09800"
     CDS             6358..8673
                     /locus_tag="VFX87_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX4995253.1"
                     /translation="MRIAIGCDNTGLALKEPLVAALEADGHDVLDLGTFSTDPVDYPD
                     YARAVGQAVLRGFVDASLLLCASGTGGVIAANKMRGIRAAMCPDVDSARRSREEVDAN
                     VLCLAATGLEARTAIEIAQAFIGTQFQAGDPQSRQVAKIGQLEAGLLGADKAPLQPVE
                     EKGSPQATPPRPAAAKPVTPMREASPPPAAAPSPPPAPPRPMAPVVTAPALVLPDPLK
                     LPAVEETLRFLEQHEFLDRLWVKDATLWKGDAATVRSRLGWLTSPTIMRSHAEDIRTF
                     ADEIRRLQYSQVVLLGMGGSSLTGEVLNETFGSKMGFPDMLVLDSTDPGAVKHILETC
                     NLGRALFLVSSKSGTTAETLAAYSFFRGQVEAAASPRPGMQFVAITDPGRPLDKLATE
                     TGFRRTFLNPASIGGRFSALSFFGLVPAAMIGVDIKILLERAHGMVETCGNEGGVRGN
                     PAVQLGAVLAGLARAGRDKVTLVLSEKIRALGSWIEQLLAESLGKDGTGLVPVADEPL
                     GAPSVYGDDRIFVSIVLEGDTVHDSALAKLSDAGHPVLRLALKDPMDLGAEFFRWELA
                     TATAGAVLGVNPFDEPDVARGKEQTSTLLTEWRRSRRLPEWPSDVEEDGLVLMTKSNK
                     KPTSVSKGLAAHLAQAVPGDYLAIQAYLTPTADAWRILQEIRVALRDRLRIATTVGWG
                     PRYLHSTGQLHKGGPTSGLFIQITGDDREDLAIPGAGYGFSTLKAAQALGDLQSLRDG
                     ARRVIRLHLTGKQSQALPQLLQMVQGLTKRL"
     gene            8679..10187
                     /gene="zwf"
                     /locus_tag="VFX87_09805"
     CDS             8679..10187
                     /gene="zwf"
                     /locus_tag="VFX87_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX4995254.1"
                     /translation="MTTLPHDESCALVIFGASGDLTRRKLIPALWSMFQSRVLPEPFA
                     VVGVARSEMTNEQFRARMREAISDFARVQPPSTRVWDRFAQALFYYSGDPANAGTYPG
                     LAGYLKQVEQERGTSGNRLFYVSTPPSVYPHLVTRLGEAGLNRPTDHTGGWVRIIIEK
                     PFGRDLASAGALNQVVASAFTEDQVYRIDHYLGKETVQNILVFRWANGIFEPLWNRNH
                     VDHVQITVGEAIGVEGRGAYYEESGALRDMIQNHILQLLCLVAMEPPVTFDSDPVRDE
                     KTKVMRAIRPIPADEVDRVAVRGQYGPGFVGGQRVVGYREEKGVSAESLAETFAALRL
                     EIENWRWAGVPFYLRTGKRLPKRASEIAVQFKQTPHLVFRRNPEILAEPNRLVLRIQP
                     DEGMSLSFGAKLPGSDLRIKPVEMEFDYGKAFGGEPPEAYERLLLDAMKGDATLYARG
                     DWVTMAWELLQPVLDTWAGGDPRKFPNYEAGTWGPAEADTLIERSGRRWRRP"
     gene            complement(10215..10625)
                     /locus_tag="VFX87_09810"
     CDS             complement(10215..10625)
                     /locus_tag="VFX87_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995255.1"
                     /translation="MRRFLVLSVTVLALTACASTPPKVQRSEFEDIPVPKGLAVDLNR
                     STIIESPSVKAARIFYKGRIEPDSLSVAFRTTLEANGWKHLSSTTASDKGTTQVYEKS
                     GSSLQVLIYESWYYTWVEMSATRMVGQASAQLKP"
     gene            10710..>11121
                     /locus_tag="VFX87_09815"
     CDS             10710..>11121
                     /locus_tag="VFX87_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995256.1"
                     /translation="MTRRAVRVTLIALLALAGLAVLTHRPVLRATGQALVVDDPRERA
                     DAIIVVAGSTPSREEAAAGLYRAGWAPQVLISRQFVPGRVQRLIDMGVRRLDFQGESL
                     AALERYGVPRDAIVTLDPPAEITETELRAVAPAAR"
BASE COUNT         1510 a         4134 c         3870 g         1607 t
ORIGIN      
        1 gatcggctcg gtgaggcgga gctcggccag ccgcacgttg ccgaacaggc tcctccagag
       61 gaagcgcacg tccagtcgct cgagccccac gaaggtctgc cccggatccg cctccgcgag
      121 acggaacttc ttgatcgcca cccggccggt gaacaggttg atgtcgatgt cctcgatcgc
      181 gacggcccgc ccggtcatcg cgggaatctg cttgagcgcc tgccacttca cgatctcggg
      241 aagcgtccag atgaaggcga tgcccaccac cagcagggcg gccagcacga tcagccagcg
      301 cttcagccgg cggctcatcg cgcgcatcga tgcctagacc ggggcctaga ccggggccta
      361 gaccggggcc tggcccgagg acggcttgag gaacgagtag acgctcgtga agtcgcgccg
      421 tccgagcccg tgggaggagg cgaggcggta gacctgctgg gccgcgggcg tcacgaagag
      481 gggcacgcgg agattccgcg ccgcctccac cgcgaggccc aggtccttgc acatgagatc
      541 ggtcatgaac ccgggctggt actcgttgct ggaggctgcg gtcggcgtga tccccggcac
      601 cggatgcatg aagcccatca ggaaggtgtt cccggaggac ttggagatga cgtcggtgac
      661 caccttggga tccatgccga acccctccgc gatgcggaac gcttcgctca ccgcgacccc
      721 gcagaccccc gcgatcaggt tgttgcagag cttgacgacc tctccgctgc cgaccgcgcc
      781 cacgtggatg acgttggtgc ccatcgcggc cagcgcgggc ttggcctcct cgaaatcggc
      841 ggcggccccg cccaccatga tggcgagcgt gccgtcggtg gcccgcggcg tcccgcccga
      901 gaccggcgcg tccatgaagc gcacgccccg ctcctgcgcg gccttcgcca ctcgctgcga
      961 caccgccggg tcgatggtcg acatgtccac gcacagcgtg cccttgcgcg cggccgccag
     1021 cacgccccca tcgccggtgt agaccgattc gacatgcggc gacgacggca gcatcgtcac
     1081 cacgatgtcc gcggtggcca ccgcctccgc cgcgctggcc gccgcggtca tgcccgccgc
     1141 ggcggcggcc ttcaccgcct ccgggttgct gtcgaacgcg gtgacggtga agcccttctt
     1201 cacgaggttc ttcgccatgg gcagccccat ggtgcccacg ccgatgaagc cgatgcgcgt
     1261 catgccttgc ctccctggga tcggagcgag acgcccgccc actcctcgat gatccgggcg
     1321 atcgcggtgt ggtcctgctc ggccatgccg cgggccatgg cgacgcggta gacctcggcg
     1381 gcggcgcggg cgaccggcga gggcaccccc gcctcctggg cgagacggcc gtagccgtcc
     1441 aggtccttca tcatgaggcg gatcgcgaag cccgcgtcga aggcgccgtt caggatgaag
     1501 cgcgggaact tgttgtcgct cgcgttggag cgcccgctgg aggcctgcag gatctccacc
     1561 gcgcgcgtgg gatccacccc ggcccgggcc gccaccgcga ccgcctcgat ggtcagcgcg
     1621 aggatgccgc ccgagcacat gttgttcacc agcttgatcg cgtggcccgc gcccacggga
     1681 cccgcgtgga agatgttctt gcccatgcgg gccagcaccg gacgcgcgcg ctcgaggagc
     1741 gcgggatcgc cgccgaccat gatggcgagc gtaccctcga cggcgccgcg caccccgccc
     1801 gacaccggcg cgtcgatgag ggcggagccg cgcgcggcca cctcccgctc gagctcgcgc
     1861 gtgaccgagg gatcggcgga ggtcatctcg agcacgaggc tccccggccg cagggcgtcg
     1921 agcagcccct tgctgcccag cagcaccgcc cgcacctcgc gcgaggtcgg caggatcgtg
     1981 atgacggtct gcgcggcggc cgcgacctcg gcgggcgacg cggcccacgt ggcgccgcgg
     2041 gccagcagcg ggcgcgccgc gtcctgccgg atgtcgtgga cgaccagcgt gtagcccccg
     2101 tccagcaggc ggcccgccat cggcacgccc atgttcccga gcccgatgaa cccgatctgc
     2161 tcactcatgc cggctgtcgt gcctcctgta tgctcgtgcc catgctgctc gggttggtgc
     2221 tcatcgcgat cgccgccgcg tcgtggggca cgaccggcgc caccatgact ctgctctccc
     2281 gcggggccgc cgcgtcgccg cttctcgtcg gctggagccg cctcgccatc gccgcgcccg
     2341 tcctcctact ggccgccgcc ggtgtcggcc gcttcggcgc gcgccatccc acactccgcc
     2401 cctggccgtc gctcgccgat ctgccgctct gcgccgcgct cggcctggcc atggccgcct
     2461 accaggtctg ctacttccgc gcggtcaccc tcgtcggcgt ggcgcccgcc gcgctcctcg
     2521 ccatctgctc cgccccgctc ctcatcgcag tgctggccgc cctcttcctc ggagagcgat
     2581 tgaccccgat cgtgcggcta tcgctcggga tggcggtcgt cgggaccgcg ctgctcgtgg
     2641 tcggcccgcg tggattcggc gagatcgcgg gccacttcgg gctgggagcg gttctcgcgc
     2701 tcggcgccgg ggtatcttac gccgtgtacg cggtggccgc aaaggggctt ctggcgcgcg
     2761 tcacaccgct cgcggtgtcc gcgatcacct tcgcgctcgc cgcgctgttc ctcgcgcccg
     2821 cgctgctcgg cgagacggcg cctgcgcgcg ccctggcggc gggctggccg ctgctgctct
     2881 acctggggat cgggcccacc gcggtggcct acgtgatgtt caccgcgggc ctgggtcgcg
     2941 tgcccgcgac cgcggccggg atcgtctcgc tgctcgagcc gctcaccgcc accacgctcg
     3001 gcctgctcgc cttcggcgag cgcctggggc cgatcggcgt cgccggcgct gttcttctcc
     3061 tcgccgccct cgcgctcctg accaccgcgc gccgctaggg cggggtcagg tcttacatta
     3121 cgacattctt atggcacccg gcgaaacgcc cggcgcggaa atgtcgtaat gtaagacctg
     3181 accccactag atgctgaccc cactagatgc ggtcgaggac gcggctgacc gcgcggctga
     3241 acgtgcagtt gggcaccacg caggagtgct tgcccgagcc gccgcccacc atcacgtaga
     3301 cgtcctccgc cgcgggcacg cgcggcagcc gcaccgactc gtcggtctcc ttggccatcc
     3361 acgtgggcca gtcgtgcatg ccccacatgc caccgagctt catgcgcccg agcggcatgc
     3421 gcgcgttctc gaagacgaag cgctgcacgt cggcgcgcgt gaacccgtcg gccgcgatgg
     3481 tggcggcgtg ctcgggcccc agcacgacca ggatctggct ctgcgaaagg taccagccca
     3541 cgttcgagcc cagcgtggtg gccacgtcgg ccacgttgtt gaggatgccg ggcgcggtgg
     3601 tggacacgtg gtcgttgacg ttgtgcgggc cctcgccgcc gaacacggtg accacgctct
     3661 ggtccgcctt gaacccgagc gccacgtgca gcggctccca cggcgagtcc tcgcgctcgg
     3721 tgatcgcgta ggagaacttg gccgggctgc cctgggtggc catgtcgccg cggccgggcc
     3781 agccgccgcc cacgttcatc aggatcagcc ggatcgcacg cccgatggtg gcattggcac
     3841 gaaagccggg cccgaagagg ccggagcccg agtgcagccc gatcgtgcgc gcgtagggtc
     3901 cggtcacgac gaccagcggc gccaccggat gggtggtggc ctgcacgccg tagaggttga
     3961 aggcgggatc gagcagggcc tgggcggcgg ccacgatcac cgggaagtac gcgggctcgc
     4021 agcccgccat gaccgcgttc accgcgagct tctcgaccgc gcactcgcgc cagagcggcg
     4081 gcattacccc gagcgattcg gcggccttgg tcttggctcc cgccagcatc gcgcgcaccc
     4141 gctccgcggt gggcgggacg aacgggagcc cgtcgcacca ctcccgcttg cagaactccg
     4201 cgtagacgtc ttcttcgggc agggtcagcg tcgcctcgac cgccgcgctc atgaggggct
     4261 cagcgggcgc ccagcatggc gccgagctgc tccaccgcct gtgcgatgcg gcggctcacg
     4321 ccctccgacg cttcaccgcc gagcggatgc tggacgatga gcagcggcag cccggcggcg
     4381 cccagcgcca ccatctcgcc gagggccagg cgctggaaca cggtggtggt gatcaatccc
     4441 gcgggcgtgc ccgccttcgc cagctccagc gagtcgtgga gactccacga cgtgcaggag
     4501 cctcagtccg cggatccggc gaagacgaga tcgcactcct tcgacagctc ctcgatgatc
     4561 gagtgagcgg cgccggtggc cgcgttggcc ttccggagga tcaccacgcg cttgacgccg
     4621 tgacgctcct tgaggacctc gccgatgccg gtgaccaggc ggtcgaagtt gtgcttggta
     4681 ttgtcgagga agcccaccgt gaggccgcgc agatcggcgg gtagcggcgg aatcgccgtg
     4741 gtcgccgtgc gaagctcgcc caccgggctc agcaccgtga tgaggggcat gtcggtctcc
     4801 tgcctgtcgg gtgaacggcg tggaccgggc ccgagcccgg atccattgcg tcgtatagta
     4861 ccgcacatgc tcgtcctggc gctggacgtc ggcaccagct ccgcccgcgc ccgcgtcttc
     4921 gacgaggccg gccgcgcgca gccgggcgcg gaggggcagg tcacctacga gccgcgcacc
     4981 accccggacg gcggcgtcga gctggacgcc gatgccctgc tggccgcagt gacgagcgcg
     5041 ctcgacacct gcctcgccgg gtgcggcgcg cgcgcagccg agatcgcggc ggtgggcgcg
     5101 tcggtattct ggcacagcct gctcgcgctc gacggcgcgg gccgcccgct caccccggtc
     5161 atcacctggg ccgacacccg cagcgcaccc gcggccgccg cgctgcggcg cgcccacgac
     5221 gagcgccgca ttcacgcgcg caccggcgcg ccagtgcatc ccgcgttctt tccggccaag
     5281 ctccgctggc tgcacgagac gcgccccgac gtcttcgcgc gcgccgccat ctggtgcggc
     5341 ttcggcgagt acctgcacgc gcggctgacc ggccgcctcg ccacgagcct ctcgatggcc
     5401 tccggcaccg gcctgctcga tcagatcgga ggcggctggg atggcgatct gctggccgcg
     5461 tccgggatcg cggccgcgca cctcccgccc atcgacgaca ccgccgcgcc cggcctgatc
     5521 gcgccgcacg ccgcccgctg gccggcgctc gcgcgcgttg cctggcatcc ggcgcgcggc
     5581 gacggcgcgt gctcgaacgt gggctccgac tgctggggcc cggggcgggt cgcgctcaat
     5641 gtagggacgt ccgccgccct gcgcgtcgtc ctgcccgagc cgctgggccc gcacgggacc
     5701 acgccgtggg ggctctggcg ctaccgggtg gacgcccggc gcgcgctggt cggtggcgcg
     5761 acgtcggagg gcggcaacgt gctggcgtgg tgccgccggc atctggccct gcccgacgat
     5821 cccgcggccc tcgaggcggc gctcggcgcg gccgagccgg acgggcacgg gctcaccgcg
     5881 ctcccattcc tggcgggcga gcggagcccg ggctggcggc ccgaggcgcg ggccgccgtc
     5941 acgggcctct ccctggatac gggggccctc gacatcaccc gggcgctcct cgagagcgtg
     6001 gcgttccgcc tggccgaggt ctacgaccgg ctgcgcccgc tggcagccgc cgatcacgtc
     6061 gtggtgggct cgggcggggc cctggcccat tccccggcct gggcccagat cgtgaccgat
     6121 gccctcggcg tcccggtggc cctcggaggc gacgcggagg cctcggcccg gggcgcggcc
     6181 ctgctggccc tcgaggcgct gggtgtggcc gtcccggggg ctccgccgcc gctccgggtc
     6241 ctccagccgg cccccgagcg ccatgcccgg taccgggcgg cgcgggagcg gcagcgcctg
     6301 gcgtacgaca acattgtcgg ctttaaccac tcctgagaga atagccgctg cgggaccatg
     6361 cgaatcgcaa tcggctgcga caacaccggc ttggccctta aagagcccct cgtggcggcc
     6421 ctcgaggccg acgggcacga cgtcctcgac ctcggcacct tctcgacgga cccggtggac
     6481 tatccggact acgcccgggc ggtcggccag gcggtgctcc ggggcttcgt ggacgcgagc
     6541 ctgctcctct gcgccagcgg gaccggcggg gtgatcgcgg ccaacaagat gcggggcatc
     6601 cgcgcggcga tgtgtcccga cgtggactcc gcacgccgca gccgagagga agtggacgcc
     6661 aacgtgctct gcctcgccgc gaccgggctc gaggccagga ccgccatcga gattgcccag
     6721 gccttcatcg ggacccaatt ccaggcgggt gacccgcagt cgcggcaggt cgccaagatc
     6781 ggccagctcg aggccgggct gctgggagcc gacaaggccc cgctgcagcc cgtggaagaa
     6841 aagggcagcc cgcaggccac cccaccccgg ccggcggccg cgaagccggt cacgcccatg
     6901 cgcgaggcgt cgcccccccc ggcggcggca ccgtccccgc cccccgcgcc gccccgcccc
     6961 atggccccag tggtcacggc gcccgcgctc gtgctgccgg atccgctcaa gctgcccgcg
     7021 gtggaggaga cgctccgctt cctcgagcag cacgagttcc tggatcgcct gtgggtgaag
     7081 gacgccacgc tgtggaaggg cgacgccgcg accgtgcgca gccgcctcgg ctggctcacc
     7141 tcgcccacca tcatgcggag ccacgccgaa gacatccgca cgttcgccga cgagatccgc
     7201 cggctgcagt actcccaggt ggtgctcctc ggcatgggcg gctcctccct gaccggagag
     7261 gtcctgaacg agaccttcgg ctccaagatg ggcttcccgg acatgctcgt gctcgactcc
     7321 acggatccgg gcgcggtcaa gcacatcctc gagacctgca acctggggcg cgcgctcttc
     7381 ctggtctcga gcaagtccgg caccaccgcc gagaccctgg ccgcgtactc gttcttccgc
     7441 ggccaggtcg aggccgcggc gtcgccgcga cccggcatgc agttcgtggc catcaccgac
     7501 cccgggcggc cgctggacaa gctggccacc gagaccggct ttcgccgcac cttcctcaat
     7561 cccgcgtcca tcggcgggcg cttctcggcg ctctcgttct tcgggctggt gcccgcggcg
     7621 atgatcggcg tggacatcaa gatcctgctc gagcgcgcgc acggcatggt ggagacgtgc
     7681 ggcaacgagg ggggcgtgcg cggcaatccc gccgtgcagc tcggggcggt gctggccggg
     7741 ctggcccggg ccggccgcga caaggtcacc ctggtgctct ccgagaagat ccgcgcgctc
     7801 gggagctgga tcgagcagct cctcgccgag tcgctcggca aggacggcac cggcctcgtg
     7861 ccggtggcgg acgagcccct cggggcgccg agcgtatacg gcgacgaccg gatcttcgtg
     7921 tccatcgtgc tcgagggcga caccgtgcac gactcggccc tcgcgaagct ttcggacgcc
     7981 gggcaccccg tgctgcggct ggccctgaag gaccccatgg atctcggcgc cgagttcttc
     8041 cggtgggagc tggccaccgc caccgcgggc gccgtgctcg gcgtgaatcc cttcgacgag
     8101 cccgacgtgg cgcgcggcaa ggagcagacg agcacgctgc tcaccgagtg gcggcgctcg
     8161 cggcgcctgc ccgagtggcc atcggacgtc gaggaggacg gcctggtgct catgaccaag
     8221 tcgaacaaga agccgacgtc ggtcagcaag ggcctggccg cgcacctcgc gcaggccgtc
     8281 cccggcgact acctggccat ccaggcctat ctcacgccga ccgcggacgc ttggcggatc
     8341 ctccaggaga tccgggtggc cctgcgcgac cggctgcgca tcgcgaccac ggtcggctgg
     8401 ggcccgcgct acctccactc cacgggccag ctccacaagg gcgggccgac gagcggcctc
     8461 ttcatccaga tcaccgggga cgaccgtgaa gatctggcca tccccggcgc gggatacgga
     8521 ttctcgaccc tgaaggccgc ccaggcgctg ggcgatctcc agagtctgcg cgacggagcg
     8581 cgccgggtga tccggcttca tctcaccggc aagcagtccc aggccctgcc gcagctactc
     8641 cagatggtcc agggactgac caagaggctc tgacgcgcgt gacgactttg ccccacgacg
     8701 aatcctgcgc cctggtcatc ttcggcgcct cgggcgatct cacccgacgc aagctcatcc
     8761 ccgcgctctg gtccatgttt cagagtcgcg tgctcccgga gccgttcgcg gtggtcggcg
     8821 tcgcgcgctc cgagatgacc aacgagcagt tccgcgcccg tatgcgcgag gccatctccg
     8881 acttcgcgcg cgtgcaaccg ccctcgaccc gcgtgtggga ccgcttcgcg caggccctct
     8941 tctactactc cggcgatccc gccaacgccg gcacgtaccc cgggctcgcg ggctacctga
     9001 agcaggtcga gcaggagcgc ggcaccagcg gcaaccggct cttctacgtc tccaccccgc
     9061 cctcggtgta cccgcacctc gtcacgcgcc tgggcgaggc gggcctcaac cgcccgaccg
     9121 accacaccgg cgggtgggtg cgcatcatca tcgagaagcc gttcggccgc gacctcgcct
     9181 ctgcgggcgc gctcaaccag gtggtcgcct ccgcgttcac cgaggaccag gtctaccgga
     9241 tcgatcacta cctgggcaag gagacggtcc agaacatcct cgtgttccgg tgggccaacg
     9301 gcatcttcga gccgctgtgg aaccgcaatc atgtggatca cgtgcagatc acggtgggcg
     9361 aggccatcgg ggtcgagggc cgcggcgcct actacgagga atcgggtgcg ctgcgcgaca
     9421 tgatccagaa ccacatcctg cagctcctct gcctggtcgc gatggagccg ccggtcacct
     9481 tcgactccga tccggtccgc gacgaaaaga ccaaggtgat gcgcgcgatc cgccccatcc
     9541 cggccgacga ggtggaccgc gtcgcggtgc gcggccagta cggacccggc ttcgtgggcg
     9601 ggcagcgggt ggtgggctac cgcgaggaga agggcgtctc cgccgaatcc ctcgccgaga
     9661 ccttcgcggc cctccggctc gagatcgaga actggcggtg ggcgggcgtg cccttctatc
     9721 tgcgcaccgg caagcggctg cccaagcgcg cgagcgagat cgcggtgcag ttcaagcaga
     9781 caccccacct cgtgttccgc cgcaatcccg agatcctcgc cgagcccaat cgcctcgtgc
     9841 tccgcatcca gcccgacgaa ggcatgtcgc tctccttcgg ggccaagctg ccgggctccg
     9901 acctgcggat caagccggtg gagatggagt tcgactacgg caaggccttc ggcggcgagc
     9961 cgcccgaggc gtacgagcgg ctgctgctcg acgcgatgaa gggagacgcc accctctacg
    10021 cgcggggcga ctgggtcacg atggcgtggg agctgctgca gcccgtcctc gacacctggg
    10081 ccggcggaga tccccgcaag tttcccaact acgaggccgg cacctggggc ccggccgagg
    10141 ccgacacgct gatcgagcgc agcggccggc gctggcggcg cccctgagcg ggcgccgccg
    10201 ccgtggcgcc ggaactacgg cttgagctgc gccgacgctt gaccgaccat ccgcgtcgcc
    10261 gacatctcga cccaggtgta gtaccagctc tcgtagatca ggacctggag cgagctgccg
    10321 gacttctcgt acacctgcgt ggtgcccttg tcggacgcgg tcgtcgacga caggtgcttc
    10381 cagccgttgg cctcgagcgt cgtgcggaac gcgacggaga ggctatccgg ctcgatgcgg
    10441 cccttgtaga agatgcgggc cgccttcacc gacggcgact cgatgatcgt ggagcggttc
    10501 aggtccaccg ccaggccctt gggcaccggg atgtcctcga actcgctgcg ctgcaccttg
    10561 ggcggcgtcg acgcgcacgc ggtcagcgcg agaacggtca cggagaggac gagaaaacgt
    10621 cgcatgggta gtacctcctg ccggtcatcg tacagtccgt ggggggtccg ccccaagctt
    10681 ttggaagcgc ggacgccgcg cgagttccgg tgacccggcg cgcggtccgg gtcacgctga
    10741 tcgccctgct cgcgctcgcg ggcctggccg tcctcaccca ccgtcccgtg ctgcgcgcga
    10801 ccggacaggc tctcgtggtg gacgatccgc gcgagcgcgc cgacgcgatc atcgtggtgg
    10861 cgggcagcac gcccagccgg gaggaggcgg cggcagggct ataccgcgcg ggctgggccc
    10921 cgcaggtgct gatctcgcgc cagttcgtgc ccgggcgcgt ccagcggctc atcgacatgg
    10981 gagtccgccg gctcgacttc cagggggaat cgctggcggc gctcgagagg tacggcgtgc
    11041 cccgggacgc catcgtcacc ctcgacccgc cggcggagat cacggagacg gagctgcgcg
    11101 cggtggcccc ggcggcccgg g
//
LOCUS       DASUFA010000266         9547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1307778, whole genome shotgun sequence.
ACCESSION   DASUFA010000266 DASUFA010000000
VERSION     DASUFA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307778"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFX87_09820"
     CDS             <1..657
                     /locus_tag="VFX87_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4995257.1"
                     /translation="NYVFSTRTLLRELYADAAREGSTHDFGRDVLPSLVNRLDMFAYD
                     FQSNQMPGEPSGAAAYWRDVGTLEAYYEAHMDLRAVSPALNLYNRQWPLRTASYADPP
                     AKFTFDDDGRRGQAIDSTVSGGCILSGGMVRNSVLGRNVRVHTGAVVEDSVILDNCDI
                     GRRAKVRRAILDKNVRIPEGATVGYDLELDKRRYHVTESGIVVVEGLRSSMDISSIVI
                     "
     gene            complement(1002..1964)
                     /locus_tag="VFX87_09825"
     CDS             complement(1002..1964)
                     /locus_tag="VFX87_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4995258.1"
                     /translation="MKILFVPNLIVSGLTDADRANILEAAGPGARIVEVKDKTAQRRE
                     LPDTDVIFGRVNPDLFSLATRLVYYHSIGAGVDSILTPELINHDVPLASEKGEVGIHL
                     AEHAFALLLALTRGLHTAVRSPDYELREPIRVHQRELYEQTMGIVGFGGTGRAVARRA
                     VGFGMRVLGVDIERVDPEPGVEAIWPADRLADLLGASDVVVIGLPLTKATHHLFTRER
                     FLQMKRTAILINVTRGEIVRGEDVLAALEEGLIWGVGLDVTDPEPLPKNHPLWTHPRA
                     IITPHTAGGSPRRAGRVIATFCENLRRMRNGRPLLALIDKQKGY"
     gene            complement(1961..2935)
                     /locus_tag="VFX87_09830"
     CDS             complement(1961..2935)
                     /locus_tag="VFX87_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4995259.1"
                     /translation="MATKILFAPKQSDAVIEIARSLTPAGFELLIAEFGTPEFYAAAG
                     QAEYYLGLARQMGGEFFRSAPNLKLVQLLSAGYDRVDIEAARKAGVPVSNNGGANAIA
                     VAEHTLMLILAVLKRVVRFHNDVVAGKWRVGGFDDQRVYELSRRTLGIVGLGNIGKKV
                     ARRAAAFDMTVQYYDIARLTEAEEDALGVRFVLLPELLRTSDVVSLHVPLDDSTRHML
                     STAQFAQMKPGAILINTCRGPVVDEAALYQALKSGQVAAAGLDVLVDEPPAKDHPLFT
                     LPNVTLTPHSAGPTWENWTARFRNGFDNIQRVAAGRAPKWVIPELAQP"
     gene            complement(2937..3278)
                     /locus_tag="VFX87_09835"
     CDS             complement(2937..3278)
                     /locus_tag="VFX87_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4995260.1"
                     /translation="MKTFRLYTGKDGQSHVEPIDLRRTPSWTSGLATTQISFRESPVG
                     NFIDWHPAPRRQFVIILSGQLEIGLGDGSKHVFGPGDARLVEDTTGRGHTTRVHGSEP
                     CLTATIPLEEQ"
     gene            3461..5929
                     /locus_tag="VFX87_09840"
     CDS             3461..5929
                     /locus_tag="VFX87_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995261.1"
                     /translation="MTWAVHFTLAPRWLDPAESEGSITPFLTLYAVHDAMLKPMPSGP
                     SAGSLAESWTVSPNGLVYDFTLRANARFHNGERVTAEDVKFSFERYRGANAGAFKERV
                     QEVRVLDARRVQFHLKEPWSDFITFYGRTASTAGWIVPKKYVEQVGDEGFKKAPVGAG
                     PYKVVSFTPGVELQLEAFDGYWRKAPAVKKLIFRSLPDETTRAAALKRGDVDVAYFLN
                     GPIAEEVRRTPGLRLMAVRSNTVFFLDFRGQWEAGSPWQDPRVRAAASLAIDRRAMND
                     AEQLGFGGITGNVVPRTLEFALPIEADPYDPARARRLLAEAGFPRGLDAGDFTIAPPY
                     EGTGEAIASYLAAVGIRVKIRTMERAAFFSAWPQGKLIQRILHEKKIFAPIWENGFIR
                     GVGPRVEEPALSFPRSRTRRPTKTSGSSPEEAPMRYEMISADCHLDLCWLPPDLFTTN
                     ATSALRDRMPHVIDQPKGPTWVTKKGSNLGLACGMGSAGREYVPGRIHRADRMAETGL
                     YADGKRGIRRLTVPELRLKDQDLDGVQAEVLYGILGATGRMNDPEAVVEVMRVYNEWL
                     ADFCSTHPERFAGLASIPNTPIDAAIAEVERVAKRGAVRGLDIANSADLTPLWDPYWN
                     PLWEVISASGLPLHFHTIGSRLPEDMQKLVFLGSDLTRAGADVTDAQKRAARIAFATH
                     ITGFQINMANILMAMIYGGVLERFPRLRVVLGEAGIGWIPYILWRMDGEWEDQFKDLS
                     LTMPPSEYWKRQVWATYQTDPVGVKLVEELGVDRIMWGSDFPHPDGVWPDSREYVARE
                     LGHLPAATRRAIVCDNAARLYNFT"
     gene            complement(5983..6504)
                     /locus_tag="VFX87_09845"
     CDS             complement(5983..6504)
                     /locus_tag="VFX87_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4995262.1"
                     /translation="MPTPHRHLALLGVLLLTLLAIALPDPAGAAGGGGGEGATPAKPE
                     DPQYTAAVKAIKANEYAKAIPLLQGVVSRDDKNADAYNWLAYATRKTGDPAASLPLYQ
                     KALAIDPKHKGAHEYIGEAYLMLDNLPKAKEHLSTLNSLCFITCGEYRDLKRAVEVYE
                     KSGGKSKPTAASK"
     gene            complement(6569..7351)
                     /locus_tag="VFX87_09850"
     CDS             complement(6569..7351)
                     /locus_tag="VFX87_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995263.1"
                     /translation="MKLADRIAIVTGAAKGMGRDICLALAGEGAHVVLAAREAAPLET
                     VAREVEATGRRALAVSCDVVDEAAVERLVARARATFGGRVDILVNAAGVTGPVETPVW
                     EIRAEDWDQVLGVNVRGTFLPTKHVLPVMIAQRYGKIVNISGTSGLRGYKLRAAYSAS
                     KWALRGFTRTVALEVGPYNINVNALHPGIVTGERMARLCRDKAKKRGTTPEAIHAEYV
                     AEMALRRVTTQEDIARAVLFLVSDDSKNMTGQSVTVDGGWDV"
     gene            complement(7348..8991)
                     /locus_tag="VFX87_09855"
     CDS             complement(7348..8991)
                     /locus_tag="VFX87_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4995264.1"
                     /translation="MTQPSTTRLSPITLEVVRHALYAIAEEMSVIIMRSARSPLLKEA
                     GDLSSALTDASGRLIAQGRDIPIHMGVMGFTVKELLKRVPLEELHEGDVWFLNLPEVG
                     GNHLPDVKAVRPVFHQGRLIAFAINLAHWADIGGAVPGSYVPSATECYLEGLRIAPIR
                     LFTAAGPERHAIDLVLSNVRGREEREGDMLAQFAANDVAARRLVELAERYGADTLAAC
                     FERLHAESEQQMRAAIAALPDGVWEGLDHLDDDGVDDRPIRVAVRVEIHGDQATFDFT
                     GTAPQARGPVNTTYFIACSAVYYAMKALVAPDVPPNDGCYRPLTVVVPPGTVLSADPD
                     RPVVGGNHETSQRVVDAIFRALAPALADRISAGGPTTAGLMIFGSRRPDGGWGIFYEV
                     HGGGAGATRGADGSSATRVHMSNVMNTPVEVIEHEYPIVVEEQALRDGSGGDGRQRGG
                     LGLRRAYRVTADEVSLTTMLERRVIPPWGIAGGADGLPFRVTLNPGPRARDLRGKETV
                     RLSAGDLVVIETSGGGGFGPPAERATAAREADRREGYTR"
     gene            complement(8988..>9547)
                     /locus_tag="VFX87_09860"
     CDS             complement(8988..>9547)
                     /locus_tag="VFX87_09860"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995265.1"
                     /translation="RALEEQCLRPILEEGHAVSAVAVQRSLDVRYVGQNYEIELPWER
                     DPAGLRPAFEARHRRLYGYATGEGVECVNLRVVARLGAAPGTPHPGPLPGGGRESPLN
                     RLPLGGRGQGEGAVSLVGSQRAYFPETGEVTMPRYDRSALAPGAVVTGPAVIEDEWST
                     IIVFPGQRAAADAQGHLILDLEPAT"
BASE COUNT         1392 a         3338 c         3364 g         1453 t
ORIGIN      
        1 aactacgtct tctcgacccg gacgctgctg cgcgagctct acgcggacgc ggcccgcgag
       61 ggcagcaccc acgacttcgg gcgggacgtg ctgccctcgc tggtgaaccg cctcgacatg
      121 ttcgcctacg acttccagtc caaccagatg cccggggagc cctcgggtgc ggcggcctac
      181 tggcgcgacg tgggcacgct ggaggcctac tacgaggccc acatggacct gcgcgcggtg
      241 agcccggcgc tgaacctcta caaccgccag tggccgctgc ggacggcgag ctatgccgac
      301 ccgcccgcca agttcacctt cgacgacgac ggccgccgtg gccaggccat cgactcgacg
      361 gtctcgggcg gctgcatcct ctccgggggc atggtgcgca actcggtgct ggggcggaac
      421 gtgcgggtgc acaccggcgc ggtggtggag gactcggtga tcctcgacaa ctgcgacatc
      481 ggccggcgcg cgaaggtccg tcgagccatc ctcgacaaga acgtgcgcat ccccgagggg
      541 gcgacggtcg gctacgacct cgagctggac aagcggcggt accacgtgac cgagtcgggc
      601 atcgtcgtcg tcgagggcct ccgctcctcc atggacatct cctcgatcgt catctagccg
      661 aggtctccaa tccccagcgc tattcggagc gcatgctgtg gcggaagatc gtcgaccaca
      721 atccggagtt cgtggtgttc tcggacaagt tggccaccaa gggatactgc tcgggccgta
      781 ctcaaagagc agaccgatcg atggctcggc tccgttcacg gggtgagcac cggtgagtgg
      841 tcctatccca aggtcgagcc gaaactgttc gtctaggaag cgatcggcga taccgagggc
      901 gacctgatcg acctcaacgt gcgcgccggc aacggacgac cgatcttcgg ctccgtcatc
      961 ggccacaaca agcgccaacc cgtcctgttc cttgagctct cctagtagcc cttctgcttg
     1021 tcgatcaggg cgaggagggg gcggccgttg cgcatccggc ggaggttctc gcagaacgtg
     1081 gcgatcacgc ggccggcgcg gcggggggag ccgcccgcgg tgtgcggggt gatgatggcg
     1141 cgggggtggg tccacagcgg gtgattcttc ggcagcggct cgggatcggt cacgtcgagg
     1201 ccgacgcccc agatgagacc ttcctcgagc gcggcgagga cgtcctcgcc gcggacgatc
     1261 tcgccgcggg tcacgttgat caggatcgcg gtgcgcttca tctggaggaa gcgctcgcgg
     1321 gtgaagaggt ggtgggtagc cttggtgagc ggcagcccga tcaccaccac gtccgaggcg
     1381 ccgaggagat cggcgaggcg gtcggccggc cagatggcct ccacgcccgg ctccggatcg
     1441 acgcgctcga tatctacccc gagcacccgc atgccgaagc ccaccgcgcg ccgagccacc
     1501 gcgcggccgg tgccgccaaa tcccacgatg cccatggtct gctcgtagag ctcgcgctga
     1561 tggacgcgga tgggctcgcg cagctcgtag tcggggctgc gtaccgcggt gtggaggccg
     1621 cgggtcagcg ccagcagcag cgcgaaagcg tgctcggcca ggtggatgcc gacctcgccc
     1681 ttctcgctgg cgagcggcac gtcgtggttg atcagttcgg gggtgaggat gctatccacg
     1741 cccgctccga tcgagtggta gtagacgagg cgggtggcga gggagaacag atcgggattc
     1801 acgcgcccga agatgacatc ggtgtcgggc agctcgcggc gctgggccgt cttgtccttc
     1861 acctccacga tgcgcgcgcc ggggcccgcc gcctccagga tgttggcccg gtccgcgtcg
     1921 gtgagtccgg agacgatcag gttcgggacg aagaggatct tcacggctga gcgagctccg
     1981 ggatcaccca cttgggcgcg cggcccgcgg ccactcgctg gatgttgtcg aagccgttcc
     2041 ggaagcgcgc ggtccagttc tcccaggtgg gcccggccga gtgcggggtc agggtcacgt
     2101 tcggaagcgt gaagagcgga tggtccttgg caggcggctc gtccaccagc acgtcgagcc
     2161 cggccgccgc gacctgcccg ctcttgagcg cctggtagag cgcggcctcg tccaccaccg
     2221 ggccccggca ggtattgatc aggatcgcgc ccggcttcat ctgggcgaac tgggcggtgc
     2281 tcagcatgtg ccgggtggag tcgtcgagcg gcacgtggag gctcaccacg tcggaggtgc
     2341 gcagcagctc ggggagcagc acgaagcgga ccccgagggc gtcctcctcg gcctcggtga
     2401 ggcgcgcgat gtcgtagtac tgcacggtca tgtcgaaggc ggcggcccgc cgtgcgacct
     2461 tcttgccgat attgcccagg cccacgatgc cgagcgtccg gcgcgacagc tcgtagaccc
     2521 gctgatcgtc gaagcccccg acccgccact tccccgccac cacgtcgttg tggaagcgca
     2581 ccacccgctt cagcaccgcg aggatcagca tcagggtgtg ctcggccacc gcgatggcgt
     2641 tggcgccgcc gttgttggag accggcaccc ccgccttgcg cgcggcctcg atgtccaccc
     2701 ggtcgtagcc cgcggacagg agctgcacca gcttgaggtt gggcgcggag cggaagaact
     2761 ccccgcccat ctggcgggcc aggcccaggt agtactcggc ctgcccggcc gccgcgtaga
     2821 actcgggcgt gccgaattcg gcgatcagca gctcgaagcc ggcgggcgtg agggagcggg
     2881 cgatctcgat caccgcgtcg gactgcttgg gggcgaagag gatcttggtg gccatctcat
     2941 tgctcctcga ggggaatggt ggcggtgagg cagggctcgc tgccgtgcac ccgcgtggtg
     3001 tgcccccgcc cggtggtgtc ctccaccagc cgcgcgtcgc cgggcccgaa gacgtgcttg
     3061 gagccgtcgc ccaggccgat ctcgagctgg cccgagagga tgatgacgaa ctggcggcgc
     3121 ggagcgggat gccagtcgat gaaattgccg accggactct cccggaagga gatctgggtg
     3181 gtggcgaggc cgctcgtcca cgacggcgtc cgccggagat cgatgggctc gacgtgggac
     3241 tgcccgtcct tgccggtgta gagccggaat gtcttcatgg cgtcctcgct cctgaccgtc
     3301 ttgtggggtg agcgcgggga ttatatcatc gggccctcgg ctcgcccggg agggcccgcc
     3361 ccatgcgacg actcatcggc gtcctcgtcg tctttgcgct gcagacgtgc ctggccgcgg
     3421 ccgggtcggc gcagccgccg gacccgcgcc ggccggccag atgacctggg cggtgcactt
     3481 caccctcgcc ccccgctggc tcgatccggc cgagagcgag ggctcgatca cgccgttcct
     3541 gactctctac gcggtccacg acgcgatgct caagccgatg ccgtcggggc ccagcgcggg
     3601 cagcctggcc gaatcgtgga ccgtctcgcc caacggcctc gtctacgact tcacgttgcg
     3661 ggccaacgcg cgcttccaca acggcgagcg ggtgaccgcg gaggacgtga agttctcctt
     3721 cgagcgctac cggggggcca acgcgggcgc gttcaaggag cgcgtccagg aagtccgcgt
     3781 gctcgacgcc agacgcgtgc agtttcacct gaaggagccg tggtcggact tcatcacctt
     3841 ctacggcagg acggcgagca ccgccgggtg gatcgtgccg aagaagtacg tcgagcaggt
     3901 gggcgacgag ggcttcaaga aggccccggt cggcgcgggg ccctacaagg tggtgagctt
     3961 cacgccgggc gtggagctcc agctcgaggc cttcgacggc tactggcgga aggcccccgc
     4021 ggtgaagaag ctgatcttcc ggagcctgcc cgacgagacg acccgcgcgg ccgcgctgaa
     4081 gcgcggcgac gtggacgtcg cctacttcct caacggcccc atcgcggagg aggtacggcg
     4141 cacgccgggc ctgcgcctca tggcggtgcg tagcaacacc gtcttcttcc tcgacttccg
     4201 cggccagtgg gaggccggct cgccctggca ggatccgcgc gtacgcgcgg cggccagtct
     4261 cgccatcgat cggcgcgcca tgaacgacgc cgagcagctc ggcttcggtg gcatcaccgg
     4321 caacgtggtg ccgcggacgc tcgagttcgc gctgcccatc gaggcggacc cgtacgaccc
     4381 cgcgcgggcc cggcgcctgc tcgcagaggc cggcttcccg cgggggctcg acgcgggcga
     4441 cttcaccatc gcgccaccct acgagggcac cggcgaggcc atcgccagct acctggccgc
     4501 ggtggggatc cgggtcaaga tccgcacgat ggagcgcgcc gccttcttct cggcctggcc
     4561 ccagggtaag ctcatccagc gcatcctgca cgagaagaag atcttcgcgc ccatctggga
     4621 gaacggcttc atccgcggcg tgggccctcg cgtggaggag cccgcgctct cattcccgcg
     4681 ttcccgtact cggcgcccta cgaagacgtc cggctcaagc cctgaggagg cccccatgcg
     4741 ctacgagatg atctccgccg actgccacct cgacctctgc tggctgccgc cggatctctt
     4801 cacgaccaac gccacctccg cgctgcgcga ccgcatgccc cacgtgatcg atcagcccaa
     4861 ggggcccacg tgggtcacga agaaggggtc caatctcggg ctcgcctgcg gcatgggctc
     4921 ggccgggcgc gagtacgtgc ccggccgcat ccatcgcgcc gaccgtatgg ccgagaccgg
     4981 cctctacgcc gacggcaagc gcggcatccg gcggctgacc gtgccggagc tgcggctcaa
     5041 ggatcaagac ctcgacgggg tgcaggcgga ggtgctctac gggatcctgg gcgcgaccgg
     5101 gcgcatgaac gatccggagg cggtcgtcga ggtcatgcgg gtctacaacg agtggcttgc
     5161 cgacttctgc tccacgcacc ccgagcgctt cgccgggctc gcgtcgatcc cgaacacgcc
     5221 gatcgacgcg gcgatcgcgg aggtggagcg ggtggccaag cgcggggcgg tgcgcgggct
     5281 cgacatcgcc aactccgcgg acctgacccc gctgtgggat ccctactgga atccgctctg
     5341 ggaggtgatc agcgcgagcg gcctgccgct gcacttccac accatcggta gccggctgcc
     5401 cgaggacatg cagaaactcg tcttcctcgg ctccgatctg acccgagcgg gcgcggacgt
     5461 gaccgacgcg cagaagcgtg cggcccgtat cgccttcgcc acccacatca ccggcttcca
     5521 gatcaacatg gccaatatcc tgatggccat gatctacggc ggcgtgctgg agcgtttccc
     5581 gcgcctgcgc gtcgtcctgg gcgaggcggg gatcggctgg atcccctaca tcctctggcg
     5641 catggacggc gagtgggagg accagttcaa ggatctctcg ctcaccatgc cgcccagcga
     5701 gtactggaag cgccaggtct gggcgaccta ccagaccgat ccggtggggg taaagctggt
     5761 cgaggagctg ggcgtggacc ggatcatgtg gggctccgac ttcccgcacc ccgacggggt
     5821 gtggcccgac tcccgtgagt acgtcgcccg cgagctcggc catcttccgg ccgcgacccg
     5881 ccgcgcgatc gtctgcgaca acgccgcgcg cctctacaac ttcacctagc aggcggggtc
     5941 aggtcttaca ttccgacaat gtgtgaatgt aagacctgac ccctacttgg acgcggcggt
     6001 cggcttcgac ttgccgccgc tcttctcgta gacctcgacc gcgcgcttga ggtcgcgata
     6061 ttcgccgcac gtgatgaagc agagcgagtt gagcgtgctg aggtgctcct tggccttcgg
     6121 caggttgtcg agcatcaggt aggcctcgcc gatgtactcg tgcgcgccct tgtgcttggg
     6181 atcgatggcg agcgccttct ggtacagcgg aagcgaggcc gccgggtcgc cggtcttgcg
     6241 ggtggcgtag gccagccagt tgtaggcgtc ggcattcttg tcgtcacggg agaccacgcc
     6301 ctggagcagc gggatggcct tcgcgtactc gttggccttg atggccttca ccgccgccgt
     6361 gtactgcgga tcctccggct tggcgggggt ggctccctcg ccgcctccgc ccccggccgc
     6421 cccggccgga tccggcagcg cgatggcgag cagcgtgagc agcaggacgc cgagaagcgc
     6481 gaggtggcga tgcggtgtcg gcatggcaag ccctcccggg ccttcaacgc cggacacggt
     6541 ccggcggttc ccggcccagt gcgacgcgtc agacgtccca gccgccgtcc accgtgacgg
     6601 actggccggt catgttcttg gagtcgtcgg agaccaggaa gagcaccgca cgggcgatgt
     6661 cctcctgcgt ggtcacgcgc cggagggcca tctcggcgac gtattcggcg tggatggcct
     6721 cgggggtggt accgcgcttc ttggccttgt cgcggcagag gcgggccatg cgctccccgg
     6781 tgacgatccc cggatgcagc gcgttgacgt tgatattgta gggccccacc tcgagcgcga
     6841 cggtgcgggt gaacccgcgc agcgcccact tggaggcgga gtaggccgcg cgcagcttgt
     6901 acccgcgcag ccccgaggtg ccgctgatgt tgacgatctt gccgtagcgc tgcgcgatca
     6961 tcaccggcag cacgtgcttg gtcggcagga acgtgccgcg tacgttgacg cccagcacct
     7021 ggtcccagtc ctccgcccgg atctcccaga ccggagtctc gaccgggccg gtcacgcccg
     7081 ccgcgttcac caggatgtcc acccgcccgc cgaaggtcgc gcgggcgcgg gcgacgaggc
     7141 gctccaccgc ggcctcgtcc accacgtcgc aggaaaccgc cagggcgcgc cggccggtcg
     7201 cctcgacctc gcgcgccacc gtctcgagcg gcgcggcctc ccgcgcggcc agcaccacgt
     7261 gcgcgccctc gccggcgagc gcgaggcaga tgtcgcggcc catgcccttg gccgcgccgg
     7321 tgacgatggc gatgcggtcg gccagcttca tcgcgtgtag ccctcccggc gatcggcctc
     7381 gcgggccgcg gtcgcccgct cggcgggcgg gccgaagcca ccgccccccg aggtctcgat
     7441 gacgacgaga tccccggcgg agaggcgcac cgtctccttg ccgcgcaggt cgcgcgcgcg
     7501 cggtcccgga ttgagcgtca cgcggaacgg cagtccgtcg gcgccgcccg cgatgcccca
     7561 gggcgggatc acccgccgct cgagcatggt ggtgagcgag acctcgtcgg cggtcacccg
     7621 gtacgcgcga cgcaggccga gcccgccgcg ctggcggccg tcgcctcccg agccgtcgcg
     7681 gagggcctgc tcctcgacca cgatcgggta ctcgtgctcg atcacctcga ccggcgtgtt
     7741 catcacgttc gacatgtgca cgcgcgtggc ggaggagccg tccgcgccgc gtgtcgcccc
     7801 cgcgcccccc ccgtgcacct cgtaaaagat gccccagccg ccgtccggcc ggcgcgaccc
     7861 gaagatcatg agcccggccg tggtggggcc ccccgccgag atgcggtcgg cgagcgcggg
     7921 ggcgagcgcg cggaagatgg cgtccaccac gcgctgcgag gtctcgtggt tgccgcccac
     7981 cacgggccgg tccggatctg cgctcagcac ggtccccggc ggcaccacga ccgtcaaggg
     8041 ccggtagcag ccgtcgttgg gcggcacgtc gggcgcgacc agcgccttca tcgcgtagta
     8101 caccgccgag caggcgatga agtaggtcgt gttcacgggc ccgcgcgcct gcggggcggt
     8161 gccggtgaag tcgaacgtgg cctgatcccc atggatctcg acgcgcaccg ccacccggat
     8221 cggccggtcg tccaccccgt cgtcgtcgag gtggtctagg ccctcccaga ccccgtcggg
     8281 cagcgccgcg atcgccgcgc gcatctgctg ctcggattcg gcgtgcaggc gctcgaagca
     8341 ggcggcgagc gtgtcggccc cgtagcgctc ggccagctcg acgaggcgcc tcgccgccac
     8401 gtcgttggcc gcgaactggg cgagcatgtc cccttcccgc tcctcgcggc cgcgcacgtt
     8461 ggagagcacg aggtcgatgg cgtggcgctc cggccccgcc gcggtgaaca gacggatcgg
     8521 cgcgatccgg agcccttcca ggtagcactc ggtggccgac ggcacgtagc tacccggcac
     8581 cgccccaccg atgtccgccc agtgcgccag gttgatcgcg aacgcgatga gccgaccctg
     8641 gtggaagacc gggcgcaccg ccttcacgtc cggcaggtga ttgccgccca cctcgggcag
     8701 gttgaggaac cagacgtcgc cttcgtgtag ctcttccagt ggaacccgct tgaggagctc
     8761 cttcaccgtg aagcccatca cgcccatgtg gatcgggatg tcgcggccct gcgcgatcag
     8821 ccggccactg gcgtccgtca gcgcggagga gaggtcgccc gcctccttga ggagcgggga
     8881 gcgcgccgag cgcatgatga tcacgctcat ctcctcggcg atcgcgtaga gcgcgtggcg
     8941 gacgacttcc agggtgatcg ggctgaggcg cgtggtggac ggctgggtca cgtggcgggc
     9001 tccaggtcca ggatgagatg gccctgcgcg tcggcggcgg cgcgctggcc cgggaacacg
     9061 atgatcgtcg accactcgtc ctcgatcacc gcggggccgg tgacgacggc gccgggggcg
     9121 agcgcgctgc gatcgtaacg cggcatcgtg acctcgcccg tctccgggaa gtaggcgcgc
     9181 tgcgagccga cgagtgaaac cgccccctca ccctgccctc tccccccgag ggggagacgg
     9241 ttgagaggag actccctccc cccgccgggg agagggccgg ggtgaggggt ccccggcgcg
     9301 gcgccgagcc gcgccaccac gcgcaggttc acgcactcga cgccctcacc ggtggcgtaa
     9361 ccgtagaggc ggcgatgccg cgcctcgaag gcgggtcgca gtccggccgg atcccgctcc
     9421 cacggcagct cgatctcgta gttctgcccg acgtatcgaa catccaggct ccgctgcacg
     9481 gccaccgcgg agacggcgtg gccctcctcc aggatcggcc gcaggcactg ctcctcgagg
     9541 gcccgga
//
LOCUS       DASUFA010000267        17800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_987389, whole genome shotgun sequence.
ACCESSION   DASUFA010000267 DASUFA010000000
VERSION     DASUFA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 17800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17800
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987389"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFX87_09865"
     CDS             complement(<1..738)
                     /locus_tag="VFX87_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995266.1"
                     /translation="MSGSSPALAVERLKKSFGGLLVTASVDLIVEAGERRLIIGPNGA
                     GKTTLFNLVTGELRPDGGTVTLFGRDITGIPGWHRAHLGISRTYQIITLFPRETLLRN
                     VTLALLGRSPLRWNPVARLERQAALIGRAREALARVGLEHLAERPLSQTSYGERRRVE
                     IAMALAQDPRVLLLDEPFAGLSIEERRDVLRLVAGIPRDVTIVMIEHDMDVALDFAER
                     ITVLHFGQVVVEGTRSEVVAHPRTREIY"
     gene            complement(735..1724)
                     /locus_tag="VFX87_09870"
     CDS             complement(735..1724)
                     /locus_tag="VFX87_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4995267.1"
                     /translation="MKRLALAAAILVLAALPLWAGGTFYINIGSQILLYAVFALGINV
                     LVGYAGLVTLGHAGLLGIAAYTAARLLNGGHGHTAAVTVALALTLAAAALFAALALRG
                     SGSGIGFVMITLAIGQIVWGIAYRWISITNGDNGISVSARPSPLGLSLASPAHFYWAT
                     LVVFLAALGSIAMLVASPFGASLRGTRDQPRRMAALGYHVWMIRFLAFLLSGFWSGVA
                     GLLFLYYNQFVSPQAVALTASAEALLMVISGGSGTLLGPVVGAVLVVVMKNVASAYIE
                     RWNFVLGAIFVAIVVFMPEGLVPGAARLWRRATARRAAPAVAAMAAPGEGPIR"
     gene            complement(1721..2581)
                     /locus_tag="VFX87_09875"
     CDS             complement(1721..2581)
                     /locus_tag="VFX87_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4995268.1"
                     /translation="MSLWLVLAVNSVTFGGLLFLLSAGFSLIFGLMRIPNLTHGSFFM
                     LGGYLAASLIEWGLGFWPAALVSGLLVAAFGGVVERLILRRLAGAELAQVLVTLGLSF
                     MVADVCLKVWTGDPVRIDTPGALQGVTSVAGFAFPTYRLAISLVAVAFAVGLWLLMDR
                     TRLGAMIRAGVDDPEMARVAGIRVSRLFTIVFCLGAWLAGFAGVIGGPILSVYPGLDQ
                     EMLPLALVVVILGGSGSLLGSLVGSFVVGFLYNFGQAMFPELAYVVLFLPMLLVLVVR
                     PQGLFGRPAV"
     gene            complement(2599..3828)
                     /locus_tag="VFX87_09880"
     CDS             complement(2599..3828)
                     /locus_tag="VFX87_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995269.1"
                     /translation="MSLTLSRRSFLAGSTVLAAGLPTRARAQGKPIRIGLLTVKTGPL
                     AAGGIQMEQGTVRFFKDRNYTLAGRKVELVVADTGGNPAGTKTKTQELVERENVDMIF
                     GPLAAFELLAISDYTAQAKVPVLSLAAAEDMTQRKPNPYFIRASATSSQNMHPLADYA
                     AKELKYKTVITLADDFAFGHEQMAGFQRVFEDAGGRVVKKLWPPLTTPDYTPYLAQIS
                     GVDAVVQGFAGSNPLKFMKQYRDQGLKLPVLGGGPAGDDALLKSYGDEAVGMISCSAY
                     TGDLDTPSNKRLIDGMVRDYGNIPGLYAAGLYINGMVAEAALEKTGGKTDDREAVNRA
                     LRAVSLTDTPRGPFSFDHFGNVVGSFYIRRCERKGGKLVNTTIKTYPKVSQFWTYDEK
                     WFLAQPVYSRDYPPLKG"
     gene            complement(3853..4392)
                     /locus_tag="VFX87_09885"
     CDS             complement(3853..4392)
                     /locus_tag="VFX87_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4995270.1"
                     /translation="MARVLGLGHVGIYVRDLDRMVAFYRDVMGMRVTKQNRRAGVVFL
                     SADPESVDHEIALMGGRPDPADPHLINQISMRVGSLDDLRAMRKRLVAEGYRIEGVVN
                     HASAIGCYFFDPEGNRTEVFWVTGRPCWVPTATPIDIEQPDETVLAAVDRVWNQLRHV
                     PVGGRMAEESATVEVTRQP"
     gene            complement(4448..5548)
                     /locus_tag="VFX87_09890"
     CDS             complement(4448..5548)
                     /locus_tag="VFX87_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995271.1"
                     /translation="MTLFTCPSKAARGGGSRSRAGGRRPFCVDIHCHVHHPAADEMVK
                     HLLSPDREPSSRFSNELSRATNRKQMENVWTCLTSVEQRLRDMDKMGVDVQAISPSPF
                     HFLYWLEPELARKVSRSVNEHLAAIVKAHPDRFVALGHVPLQAPDLAAEELAYCVTQL
                     GFRGAEIGTNVVGAEVSRGRDRFWKKAQELDVVLFMHPNGFTQGDRLADHYFTNVIGN
                     PLDTTVALGHLVFDGVLERFPKLKLVAAHGGGYIAHYPARMDHVWGARADARTVLKNI
                     PRRSLARVYFDTIVFDRIQLEHLVKLWGADHVLAGTDYPYDMGMYDPRGFVGGAGLTP
                     ADKAKILGLNAARLLKIKAPAAPKAGRARGRR"
     gene            complement(5545..5673)
                     /locus_tag="VFX87_09895"
     CDS             complement(5545..5673)
                     /locus_tag="VFX87_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995272.1"
                     /translation="MGYEHLVNTLKAIAITAPAARAFEPIRHGQNSWYEVLAGLEP"
     gene            5672..9055
                     /locus_tag="VFX87_09900"
     CDS             5672..9055
                     /locus_tag="VFX87_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEX4995273.1"
                     /translation="MGLASTLGWQCWTAFYYGSHTMQHLNDRFLFSPSDLGSFLECEH
                     LTQLELAVALREGRRPSYENSYAELLRRKGQEHEEAFLATLRAAGRGVVEVRLAANRD
                     FETGTKRTAEAMRAGADYVYQAVFFSQGWRGIADFLERVERPSALGAWSYQVLDTKLA
                     RHPRPEHALQLSFYSQALEETQQLAPDLAYVVLGTRERVPIRLADVTAYFRRVRARFG
                     SAVATRPATGPYPCHHCSFCDFRGLCEDRLEREDHLVRVAGIHRGQVKRLFGGGINTL
                     TALAEAPPGTAVSRIAPSTFEGLREQAGLQLIRQRAGTLEWRALDVEAGRGFAALPPR
                     APGDVIFDLEGHPFFEPARGLEYLFGVLLQDGAEPRYQAFWAHDRDGERRAFEALVDL
                     IHGRLEQDPGLHVYHFSGSEPSTLKRLMAEYSTREAQVDDLLRRKVFVDLHTILRHAL
                     RAGVPSYSLKDVEALFGFTRGGAVQSGTQAILHYERWLQQRSPALLDEIAAYNLEDCR
                     ATRGLLEWLHQVRPADLPWPEAPEPHAPSPEATRALDERQRLRQELIDGAEPGTARWL
                     GGELLDYHRREARPAWWAYFDRLGKSPEELLEDTEAIAYLEVDRGTPPERRKKSLVHT
                     LIFPIQDHKLHPGAMVHDPATGRSAGEIVEIDDTTGRLRLQRGPSFASRPLPEAVVAG
                     GPIEDRTQRAALLRLAESIRAGDGRYPALRAILARERPSIRGVAPGAAVQTTDLEEMK
                     ALALGLDASYLFLQGPPGTGKTWTGARIVVHLLAGKQRVGIAAQSHKAIHNLLAEVEK
                     VARAAGVHFKGLKKSTAGNPESEYDGEFITSDSDNATFANAGPDVQLLAGTAWLFSRP
                     DLDGKLDHLVIDEAGQVSLADALAMGTAARNVILLGDPLQLAQVSQGVHPAGSGASVL
                     EHLLGDAATIPVDRGVFLERSFRMHPGVSAFISEIVYAGRLHSDESAGRRTTSFGTGI
                     RFAPVDHDGNRSSSDEEVARVAALIAGMRGGTFTDADGTTRPLRDDDFMVVAPYNAQV
                     LRLRTGLPGGVRVGTVDKFQGQEAPIVFFSMATSSGEDVPRSLSFLFSRNRLNVAISR
                     AQCLAVLVCSPRLLEARCQSIEEMQLVNALCRLVEYAGT"
     gene            complement(9267..10061)
                     /locus_tag="VFX87_09905"
     CDS             complement(9267..10061)
                     /locus_tag="VFX87_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995274.1"
                     /translation="MEIKLDERRAERRLSRDARDPERRRGERRQQPSLEGDLRARGYA
                     TVVQSETGSSRMGETTPAPAMVWRPRSSWRQRVARAARRSRVWWGLTLGLLAGVGVVI
                     VVARSIHWTPAPPPPAPQIDAQIAPPMTPPIEKASPPPLSSKAQPVVPAVPPLPASRP
                     APGRIVSMRFSGVVLSVDPRAGMLVVEDTGAAGEAGRLRVELAPDARVVLSERNDQAE
                     DLSRPFKDTVISLSDVRRGDYVVVERRGPEGKELAHSVVVTFRPMK"
     gene            complement(10175..10705)
                     /locus_tag="VFX87_09910"
     CDS             complement(10175..10705)
                     /locus_tag="VFX87_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4995275.1"
                     /translation="MAPRGFSHIGLSTLDLDRTRDFYERVLGFKAVRCDILKVKEGGQ
                     IRHIFFDTGHDQLIAFMEASGVPGIPAEYDAGINRGLGVPGVFYHFAFEAGSEAALDE
                     KRHELLAKGVEVTEVVDHDWAKSIYFKDPNGIQLEFCCYTRNLNADDARMQPRAEISV
                     RRLGLEDPVALRSARR"
     gene            complement(10739..11080)
                     /locus_tag="VFX87_09915"
     CDS             complement(10739..11080)
                     /locus_tag="VFX87_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995276.1"
                     /translation="MATGRWGPALLIGTIAVVAVDGAAADQGYVIWRSTTLDRLDWVP
                     GSRTYASREACDQAVAARQGRVARAVAFLRRIGADDTLLQMVGDRVYECRPSLTRPPA
                     RPSGSEPAQSP"
     gene            11147..11377
                     /locus_tag="VFX87_09920"
     CDS             11147..11377
                     /locus_tag="VFX87_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995277.1"
                     /translation="MITRRELERWLLREGAVRVKRADGHKHFTLRGHHVVVLGHGPQT
                     LSATSVSLVMKQLEQAGYNREQLRREWGSERS"
     gene            complement(11397..11654)
                     /locus_tag="VFX87_09925"
     CDS             complement(11397..11654)
                     /locus_tag="VFX87_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995278.1"
                     /translation="MRQSVRWLFVTSGLIAVATLGFGTAGAADKTKVDQATQQVQQGA
                     RQIGQGNVGTGVKETAKGIGNTVVEGAKFSGRTIKEFFTGK"
     gene            complement(11698..11907)
                     /locus_tag="VFX87_09930"
     CDS             complement(11698..11907)
                     /locus_tag="VFX87_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995279.1"
                     /translation="MSFACRSERRCDHELKDCATVRGEYCLVCHHNSSLATPARRSTT
                     LGPRKDRAGLSGVLREIRERRKRKA"
     gene            complement(11925..12080)
                     /locus_tag="VFX87_09935"
     CDS             complement(11925..12080)
                     /locus_tag="VFX87_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX4995280.1"
                     /translation="MFKSNFLIAFVVLVVLWALDLFVLHLTGGLIHVLLVAGVVMLIV
                     HFLRGRP"
     gene            complement(12100..12294)
                     /locus_tag="VFX87_09940"
     CDS             complement(12100..12294)
                     /locus_tag="VFX87_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995281.1"
                     /translation="MAGGVIVGVLRECHEDHIVLENGTKIFLTSKQTAGGLSPGTSLT
                     VAYTAKRDGRKVADNIWSGS"
     gene            12470..13549
                     /locus_tag="VFX87_09945"
     CDS             12470..13549
                     /locus_tag="VFX87_09945"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEX4995282.1"
                     /translation="MRIPSDRLRRLTATILARGGSEPAEADLVAAHLVDANLAGHDSH
                     GVGLIPHYVRHLKEGLVVPNTSVKAVKDDGAFLMFDGQRGFGRRVAGEAMAAAMARAR
                     DAGVVVVTLANSHHVGRVGAYGELASAAGLVSIHFVNVVDHTGLVAPFRGSDSRFSTN
                     PVCIGMPGTATQPPLLLDMATSGVAMGKVAVAKRAGKKLPDGNLIDALGAPTNDPEVM
                     YQEPRGSLLPFGAHKGYALAVLTELLAGAMSGGGTIQPENPRRGGVVNNMFSVLIDPA
                     RLAGVDWMRREIDGFVGYVKGSPPADPASPVLVPGDPERLSREARQRAGIPLDATTWE
                     EMVTAGAVMGVTRAQAEAIAAPTAD"
     gene            13566..14804
                     /locus_tag="VFX87_09950"
     CDS             13566..14804
                     /locus_tag="VFX87_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995283.1"
                     /translation="MIRILTGATLIDGTGATPVPDAAVIMNGDRITAAGPRAALSWPA
                     GAEIVDVRGRTVIPGLIDAHDHLASHGYGLATRWGLDEPASTAHLRTARVLAETLAMG
                     YTTVRDGGGLDAGFKLAIEQGLIPGPRLVLAVQIISPTGGIGDRVSPSGHDCCAAYDP
                     LLPASVANGPDAVRDVVRTMVRAGAGVIKTATTGGASSRAGHGPLDPAFTLGEMDALV
                     AESHALGRRVMCHALGGPGLRTALEAGVDTIEHGCYLDEDPTLLGQMAVQGTFFVPTL
                     TVYVYHRESLSPHVRERALALYPHHVASVRRALELGVPIAAGTDAGGHGHPRNALELK
                     YLVEAGLSPMQALRAATQWAAACLGLEREVGTIEKGRLADLVVVNGNPLDDITVLLDP
                     ARVELVWKGGVICADRRSPR"
     gene            14816..15112
                     /locus_tag="VFX87_09955"
     CDS             14816..15112
                     /locus_tag="VFX87_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995284.1"
                     /translation="MDPQQLLDELAQAANRLGVEVRTEPFETPATMGGGLCIVRGESL
                     VLIDQRAPLPDRLRALARALVELRSETVYMAPEARELIEALRAPAVPAAPRQTS"
     gene            complement(15367..15630)
                     /locus_tag="VFX87_09960"
     CDS             complement(15367..15630)
                     /locus_tag="VFX87_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995285.1"
                     /translation="MMRRLRGLWIIPACLMVVALSFGVSGAADRSKVDQATSRVERGA
                     KQIGQGELGPGFKEFFIGIGHTIVEGAKFSGETIGEFFKKASG"
     gene            15748..17175
                     /locus_tag="VFX87_09965"
     CDS             15748..17175
                     /locus_tag="VFX87_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995286.1"
                     /translation="MPDTDDSPSPSIKRRLAAILAADIAGYSRLMGEDEAATVRDLKE
                     HQAVILPLVVRYGGRIIDTAGDGILAEFPSVINATECAVEIQTVMATRNEAVPEARRM
                     RFRIGINLGDVIHDDTRIYGDGINVAARLEGIAEPGGICISRQVFDQVSRALKADFQA
                     LGPRTFKNIARPVDVFAIAPAGGDGGPGVTGANAVDLRQEVRFCTAPDGVQLAYSMIG
                     QGPPLMKTGNWMTHLEFDLESPIWRPLYRELATDHTLIRYDARGNGLSDRAVDEISFD
                     AFVRDLEAVVDAAGITRFALLGISQGCAVSIAYAVRHPERVSRLILYGGYALGWSKRA
                     RSAAQKEEDEAVLTLMRVGWGKENPAFRQVFTSQFVPGATKEQSDWFNELQRITVSGD
                     TAARIFTATGETDVTALLPRVSVPTLVLHARDDARVPFEAGRRMAAGIPGARFVALTG
                     RNHVFLESEPAFAQFLEHARAFLAG"
     gene            complement(17196..>17800)
                     /locus_tag="VFX87_09970"
     CDS             complement(17196..>17800)
                     /locus_tag="VFX87_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995287.1"
                     /translation="STLIKCIMGMVRPEAGEIFLETDGTRVDLTRKSTEEIVGMGIAL
                     VPEGRRLFPKLSVEENLLLGAYRAGARADIATNLAFAFEAFPVLAGRRRQLAGSMSGG
                     EQQMLAVARALMSSPRILLVDEPSVGLAPILVSRVIGKIREMKEQRQLTVLMAEQNFN
                     QATKIADRGYIIVHGKIEFEGRSTRELRENELVKQYYLGV"
BASE COUNT         2702 a         6435 c         6115 g         2548 t
ORIGIN      
        1 gtagatctcc cgggtccggg ggtgcgccac cacctcgctg cgggtgccct cgaccaccac
       61 ctggccgaag tgcagcacgg tgatccgctc ggcgaagtcc agcgccacgt ccatgtcgtg
      121 ctcgatcatc acgatcgtga catcgcgggg tatgccggcg accaggcgca gcacgtcacg
      181 ccgctcctcg atggagagcc cggcgaaggg ctcgtccagc agcaggaccc gggggtcctg
      241 ggccagggcc atggcgatct ccacccgccg gcgctccccg taggacgtct gggagagggg
      301 gcgctccgcg agatgctcga gcccgacgcg ggccagcgcc tcgcgcgccc ggccgatcag
      361 cgccgcctgc cgctccagcc gggcgaccgg gttccagcgc agcggcgagc gcccgaggag
      421 cgcgagcgtg acgttccgca gcagcgtctc gcgcgggaac agcgtgatga tctggtaggt
      481 gcgggagatc ccgagatgcg cgcggtgcca gcccgggatc ccggtgatgt cccggccgaa
      541 gagcgtgacc gtcccgccgt cgggccggag ctcgccggtc accaggttga agagggtcgt
      601 cttgcccgcg ccgttcgggc cgatgatcag gcgccgctcc cccgcctcca cgatgagatc
      661 caccgacgcg gtgacgagca ggccgccgaa ggacttcttc aggcgctcca ccgccagcgc
      721 gggcgacgac ccgctcaccg gatcggcccc tcgcccggcg cggccatcgc cgccaccgcc
      781 ggggccgccc gccgcgccgt cgcccgccgc cacagtcggg ccgcgccggg caccagccct
      841 tccggcatga agaccacgat cgccacgaag atggcgccga ggacgaagtt ccagcgctcg
      901 atgtacgcgc tcgcgacgtt cttcatgacg acgaccagca cggccccgac caccgggccc
      961 agcagcgtcc cgctcccgcc cgagatgacc atcaggagcg cctcggccga ggcggtgagc
     1021 gccaccgcct gcgggctcac gaactggttg tagtagagga agagcagccc ggccacgccg
     1081 ctccagaatc ccgagagcag gaacgcgagg aaccggatca tccacacgtg gtagccgagc
     1141 gcggccatgc gccgcggctg gtcgcgggtg ccgcggaggc tcgccccgaa cggcgaggcc
     1201 accagcatcg cgatggatcc cagggcggcg aggaacacca cgagggtcgc ccagtagaag
     1261 tgggccggcg aggccagcga caggccgagc gggctcggcc gcgcgctcac cgagataccg
     1321 ttgtccccgt tcgtgatgct gatccagcga taggcgatgc cccagacgat ctggccgatc
     1381 gcgagggtga tcatcacgaa gccgatcccg ctgccgctgc cgcgcagcgc gagggccgcg
     1441 aagagcgcgg ctgcggcgag cgtcagggcg agcgccaccg tgaccgccgc ggtgtggccg
     1501 tgcccgccgt tgaggaggcg ggccgcggtg taggccgcga tgccgaggag cccggcgtgg
     1561 cccagcgtga cgaggcccgc gtagcccacc aggacgttga tgccgagcgc gaagaccgcg
     1621 tagaggagga tctggctccc gatgttgatg tagaaggtgc cgccggccca gagcggcagc
     1681 gccgccagga ccaggatcgc ggcggccagc gcgaggcgct tcacaccgcc ggccgcccga
     1741 agaggccctg gggccgcacc accaggacga ggagcatggg gaggaacagc acgacgtagg
     1801 ccagctcggg gaacatcgcc tgaccgaagt tgtagaggaa gccgacgacg aagctgccga
     1861 ccaacgagcc gaggaggctg ccgctgcctc cgaggatgac cacgacgagg gccagcggga
     1921 gcatctcctg gtcgaggccg ggatagacgg agaggatcgg cccgccgatg acgccggcga
     1981 acccggccag ccaggcgccc aggcagaaga cgatggtgaa gaggcgcgag acgcggatgc
     2041 ccgcgacccg ggccatctcc ggatcgtcga cgccggcccg gatcatcgcg ccgagccgtg
     2101 tccgatccat cagcagccac agccccaccg cgaaggccac cgcgaccagg ctgatcgcga
     2161 gccggtaggt cggaaacgcg aagcccgcga ccgaggtcac gccctgcagc gcgcccggcg
     2221 tgtcgatgcg gacggggtcg ccagtccaga ccttcaggca cacgtccgcg accatgaagg
     2281 acagaccgag cgtcaccagg acctgcgcca gctcggcccc ggccagccgc cgcaggatga
     2341 gccgctcgac cacgcccccg aaggccgcga ccagcagccc gctgaccagg gccgccggcc
     2401 agaaaccgag gccccactcg atcaggctcg ccgcgagata gccgccgagc atgaagaacg
     2461 agccgtgcgt caggttcggg atccgcatca gcccgaagat cagcgagaaa ccggcggaca
     2521 gcaggaacag caggccgccg aacgtgacgc tgttgacggc cagcacgagc cagaggctca
     2581 tgacggagcg ggcgcggctc agcctttcag cggcggatag tcacgcgagt agaccggctg
     2641 cgccaggaac cacttctcgt cgtaggtcca gaactggctc accttcgggt acgtcttgat
     2701 cgtggtgttg acgagcttgc cgcccttgcg ctcgcagcgc cggatgtaga agctgccgac
     2761 gacgttgccg aagtggtcga aggagaacgg cccgcgcggc gtgtcggtga gcgacaccgc
     2821 gcgcagcgcc cggttcaccg cctcgcggtc gtcggtcttg ccgcccgtct tctcgagcgc
     2881 ggcctccgcg accatgccgt tgatgtagag gccggccgcg tagaggccgg ggatgttgcc
     2941 gtagtcgcgc accatgccgt cgatgagccg cttgttgctc ggggtgtcga ggtcgccggt
     3001 gtaggccgag caggagatca tgccgactgc ctcgtcgccg tagctcttga gcagcgcgtc
     3061 gtcgccggcc ggcccgccgc ccaggaccgg cagcttgagg ccctgatccc ggtactgctt
     3121 catgaacttg agcgggttcg agccggcgaa gccctgcacc accgcgtcca ccccgctgat
     3181 ctgggcgagg tacggcgtgt agtcgggggt ggtcagcggc ggccacagct tcttcaccac
     3241 ccggccgccc gcgtcctcga agacgcgctg gaacccggcc atctgctcgt ggccgaacgc
     3301 aaagtcgtcg gcgagggtga tgacggtctt gtatttcagc tccttcgccg cgtagtcggc
     3361 gagcgggtgc atgttctggg aagaggtggc ggaggcgcgg atgaagtacg ggttgggctt
     3421 ccgctgggtc atgtcctcgg cggcggccag gctcagcacc ggaaccttcg cctgcgcggt
     3481 gtagtcgctg atcgcgagca gctcgaaggc ggccaggggc ccgaagatca tgtccacgtt
     3541 ttcgcgctcg acgagctcct gcgtcttggt cttggtgccg gccggattgc cgccggtgtc
     3601 ggccaccacg agctcgacct tgcggccggc gagcgtgtag ttgcggtcct tgaagaagcg
     3661 caccgtgccc tgctccatct ggatgccgcc cgcggcgagc gggccggtct tcacggtgag
     3721 gaggccgatg cgaatcggct tgccctgcgc ccgggcccgg gtgggcagtc ctgcggcgag
     3781 aaccgtgcta ccggccagga acgaacggcg actgagtgtc agcgacattg ctgcctcctg
     3841 tggagaaacg ggctagggct ggcgggtcac ctcgacggtc gcgctctctt cggccatccg
     3901 cccgcccacc gggacgtggc ggagctggtt ccagacgcgg tcgaccgcgg cgagcacggt
     3961 ctcgtccggc tgctcgatat cgatgggggt ggcggtcggc acccagcagg gccggcccgt
     4021 cacccagaac acctcggtgc gattgccctc ggggtcgaag aagtagcagc cgatcgcgct
     4081 ggcgtggttg acgacgccct cgatgcggta gccctccgcg accaggcgct tgcgcatcgc
     4141 gcgaaggtcg tcgaggctgc cgacgcgcat cgagatctgg ttgatcaggt ggggatccgc
     4201 cgggtcgggc cggccgccca tgagcgcgat ctcgtggtcc accgactcgg gatcggcgct
     4261 caggaagacc acgcccgcgc gccggttctg cttggtcacg cgcatgccca tcacatcccg
     4321 gtagaacgcg accatccggt cgaggtcgcg cacgtagatc ccgacgtggc ccagccccag
     4381 cactctcgcc atcggccccc tcccccgctg ccccgcagac ctcagctcgg acgcccggag
     4441 aatactccta tcggcggccg cgcgcccgcc ccgctttcgg ggcggccggg gccttgatct
     4501 tgagcagccg cgctgcgttg agacccagga tcttcgcctt gtccgccggc gtgaggccgg
     4561 cgccccccac gaagccccgc gggtcgtaca tccccatgtc gtagggatag tcggtgccgg
     4621 cgagcacgtg gtcggcgccc cacagcttga ccagatgctc gagctggatc cggtcgaaca
     4681 cgatcgtatc gaagtacacc cgggccagcg accggcgcgg gatgttcttg aggacggtcc
     4741 gcgcgtcggc ccgcgcgccc cagacgtgat ccatccgcgc cgggtagtgg gcgatatagc
     4801 cgccgccgtg cgcggcgacc agcttcagct tcgggaaccg ctcgaggacc ccgtcgaaga
     4861 cgagatggcc cagggcgacc gtggtgtcga gcgggttgcc gatgacgttg gtgaagtagt
     4921 gatccgccag gcggtccccc tgggtgaagc cgttcggatg catgaagagg acgacgtcga
     4981 gctcctgggc cttcttccag aaccggtccc ggccccgcga gacttccgcg ccgaccacgt
     5041 tggtgccgat ctcggcgccc cggaacccga gctgcgtgac gcagtacgcg agctcctcgg
     5101 ccgccaggtc cggcgcctgc agcggcacgt gcccgagcgc gacgaaccga tcggggtgcg
     5161 ccttgacgat ggcggccagg tgctcgttca ccgagcggct caccttgcgc gccagctcgg
     5221 gctcgagcca gtacaggaaa tggaagggcg acggcgagat ggcctggacg tccacgccca
     5281 tcttgtccat gtcgcgcagc cgctgctcca cggaggtgag gcaggtccag acgttctcca
     5341 tctgcttgcg attggtggcc cgcgagagct cgttcgagaa tcgggacgag ggctcccggt
     5401 ccggcgagag caggtgcttg accatctcgt ccgcggcggg atggtgcacg tggcagtgga
     5461 tgtcgacgca gaaggggcgg cggccgcccg cgcgagagcg cgagccgccg ccgcgggcgg
     5521 ccttggatgg gcacgtgaag agcgtcatgg ctcgagcccg gcgagcactt cgtaccagct
     5581 attctgcccg tggcggatcg gctcgaaggc gcgcgccgcg ggcgcggtga tcgcgatcgc
     5641 cttcaacgtg ttcacgagat gctcgtagcc catgggcctc gcctccacgc tcggctggca
     5701 atgctggacg gcattctact atggtagcca tacgatgcag cacctgaacg accgcttcct
     5761 cttctccccc tcggacctcg gcagctttct ggaatgcgag cacctgaccc agctcgagct
     5821 ggcggtggcg ctccgggaag gccggcggcc gagctacgag aactcctatg ccgagctgct
     5881 ccggcgcaag ggccaggagc acgaggaggc cttcctcgcg acgctccgcg ccgccggccg
     5941 cggcgtggtc gaggtgcgcc tggccgccaa ccgcgacttc gagaccggga ccaagcgcac
     6001 cgcagaggcg atgcgcgcgg gcgccgacta cgtctaccag gccgtcttct tctcgcaggg
     6061 ctggcgcggg atcgcggact tcctggagcg ggtggagcgg ccctccgcgc taggcgcctg
     6121 gagctaccag gtgctggaca ccaagctcgc gcggcatccc cgcccggagc acgcgctcca
     6181 gctctccttc tatagtcagg cgctcgagga gacccagcag ctcgcccccg atctggccta
     6241 cgtcgtcctc ggcacccgcg agcgggtgcc gatccggctg gccgacgtga cagcctactt
     6301 ccggcgcgtg cgcgcgcgct tcgggagcgc ggtggcgacc cggcccgcga ccggtcccta
     6361 cccgtgccat cactgctctt tctgcgattt ccgggggctc tgcgaggacc ggctggagcg
     6421 tgaggatcac ctcgttcggg tggcgggtat ccatcggggc caggtgaagc ggctgttcgg
     6481 cggcgggatc aacaccctca ccgcgctcgc cgaggcgccc ccaggcaccg cggtgtcgag
     6541 aatcgcccct tcgacgttcg aggggctgcg cgagcaagcg ggcctgcagc tcatccggca
     6601 gcgcgcgggc acgctcgaat ggcgggcgct cgacgtcgag gccggccgtg gcttcgcggc
     6661 actgcccccg cgggcgcccg gcgacgtcat cttcgatctc gaggggcatc cgttcttcga
     6721 gcccgcgcgg ggcctcgagt acctcttcgg cgtcctcctg caggacggcg ccgagccacg
     6781 gtaccaggcc ttctgggcgc acgaccgcga cggcgagcgc cgggcgttcg aggccctggt
     6841 cgatctcatc catggccgcc tcgagcagga tcccggcctg cacgtctacc atttcagcgg
     6901 ctcggagccg agcacgctca agcggctcat ggccgagtac tccacgcgcg aggcgcaggt
     6961 ggacgacctg ctccgtcgca aggtcttcgt ggatctccac acgatccttc gccacgccct
     7021 gcgcgcgggc gtgcccagct actcgctcaa ggacgtcgag gcgctcttcg gcttcacccg
     7081 gggcggcgcc gtgcagtcgg gcacccaggc catcctccac tacgagcgct ggctgcaaca
     7141 gagaagcccg gcgctgctcg acgagatcgc ggcctacaac ctggaggact gtcgcgccac
     7201 gcggggcctg ctcgagtggc tgcatcaggt ccgccccgcc gacctgccct ggccggaagc
     7261 gccggagccg cacgcgccat cgcccgaggc cacccgagcc ctggatgagc ggcagcgtct
     7321 gcgccaggag ctgatcgacg gggccgagcc cgggactgcg cgctggctcg ggggcgagct
     7381 gctcgactac caccggcgcg aggcccgccc cgcctggtgg gcctacttcg atcggctcgg
     7441 caagtcgccg gaagagctgc tggaggacac ggaagcgatc gcgtatctcg aggtcgatcg
     7501 cggcacgccg cccgagcggc ggaagaagtc gctggtccac accctcatat ttccgatcca
     7561 ggaccacaag ctccatcctg gagcgatggt ccacgatccg gccaccggga ggagcgcggg
     7621 cgagatcgtc gagatcgatg atacgaccgg gaggctgcgc ctgcagcggg ggccgagctt
     7681 cgcctcacgg ccgctcccgg aagccgtcgt cgccggcggg ccgatcgagg accggaccca
     7741 gcgcgcggcc ctgctgcgcc tcgccgaatc gatccgggcg ggcgatgggc gctatcccgc
     7801 gctgcgggcg atcctcgccc gggagcggcc gagcatccgc ggcgttgccc caggcgcggc
     7861 ggttcagacg accgacctgg aagagatgaa ggcgctcgcc ctcgggctcg acgcgagcta
     7921 cctcttcctc cagggcccgc ccggcaccgg gaagacgtgg accggcgcac ggatcgtcgt
     7981 ccacctgctc gccggcaagc agcgggtcgg catcgcggcc cagagccaca aggccatcca
     8041 caacctgctc gccgaggtcg aaaaggtcgc ccgggccgcc ggtgtgcact tcaaggggct
     8101 caagaagtcg accgccggga acccggagtc cgagtacgac ggcgagttca tcacgagcga
     8161 ttccgacaat gccacgttcg cgaacgccgg ccccgacgtc cagctcctcg ccgggaccgc
     8221 ctggctcttc tcgcgcccgg acctggatgg caagctggac cacctggtga tcgacgaggc
     8281 cggccaggtc tcgctcgcag acgcgctggc gatgggtacc gccgcgcgca acgtgatcct
     8341 cctcggggat ccgctccagc tcgctcaggt ctctcagggt gtgcatccgg ccgggagcgg
     8401 cgcctccgtc ctcgagcacc tgctcggaga tgccgcgacg atcccggtgg accgcggcgt
     8461 cttcctcgag cgcagcttcc gcatgcaccc cggcgtctcc gcgttcatct ccgagatcgt
     8521 ctacgcgggg aggctgcact ccgacgagtc ggccgggcgc cggaccacgt ccttcggcac
     8581 cggcatccgg ttcgccccgg tcgaccacga cggcaaccgg tcctcctcgg acgaggaggt
     8641 cgcccgggtc gcggccctga tcgccggtat gcgcggcggg accttcaccg atgccgacgg
     8701 cacgacccgc ccgctccgcg acgacgactt catggtcgtg gcgccctaca acgcccaggt
     8761 gctccggctg cgcacgggcc tgcccggcgg cgtccgcgtc ggcaccgtcg acaagttcca
     8821 gggccaggaa gcgcccatcg tcttcttctc gatggccacc tccagcggcg aggacgtccc
     8881 gcgcagcctc tccttcctct tctcccgcaa ccgcctcaac gttgccatct ctcgcgccca
     8941 gtgcctcgcc gtcctggtct gctccccacg cttgctggag gcccgctgcc agtccatcga
     9001 ggagatgcag ctggtcaacg ccctctgccg cctggtcgag tacgcgggca cctaggccgg
     9061 gttcaggtct tgcgtcgctg cctagtcctc cccggccgag aaggcagacg cacagaggaa
     9121 tccgaaggca aagacgatca ccaccagcag gtacgcccag aggaagcgca tagcttgcgg
     9181 aacatcttca gggcacgacc gcgcagaagc ggcagaaaaa accgctcgcc gccgactacc
     9241 atccctgggg gtccgaccac acgtccctac ttcatcggcc ggaaggtcac cacgaccgaa
     9301 tgcgccagct ccttcccctc cggcccccgc ctctccacga cgacgtagtc gccacgcctg
     9361 acatcggaga ggctgatcac ggtgtccttg aacgggcggc tgagatcctc ggcttggtcg
     9421 ttccgctcgg acagcaccac gcgggcgtcg ggtgccagct ctacgcgaag acgtccggct
     9481 tcgccggcgg ctcccgtgtc ctcgacgacg agcatgccgg ctctcgggtc gacggacagc
     9541 acgacaccgg agaaccgcat gctgacaatc cgtcccggag ctggcctcga tgccggaagc
     9601 ggcggcacgg cgggcacgac cggctgggcc ttcgaggaga gaggaggcgg cgacgccttt
     9661 tcgattggcg gagtcatcgg cggagcgatc tgcgcatcga tctgcggagc gggcggtggc
     9721 ggagcgggcg tccagtgaat ggaccgggcc acaacgatca ccaccccgac ccccgcgagc
     9781 agcccgaggg tcagtcccca ccagacgcgg cttcgccgcg ccgctcgggc cacacgctgc
     9841 cgccagctcg accggggccg ccagaccatg gccggcgccg gcgtggtctc gcccatccgc
     9901 gatgagccgg tctcggactg gaccacggtg gcgtaccctc gggcgcggag gtctccttcc
     9961 agactgggtt gctgcctccg ctcgccccgg cgtcgttccg ggtctcgcgc gtcacgcgac
    10021 agccgacgct cggcccgccg ctcgtccagc ttgatctcca cgaggtgcgc ctcggaggat
    10081 cgggcgccca ccgtggtcat cagatacccg accagcaagg gctgctcccg cgccaccacg
    10141 taaagaactc gctccatcga ggtggccggt ctgcttaccg tctcgccgag cgcagcgcga
    10201 ccggatcctc cagccccagg cgacgcaccg agatctcggc gcggggctgc atgcgggcat
    10261 cgtccgcgtt caagttccgg gtgtagcagc agaactcgag ctggatcccg ttcggatcct
    10321 tgaagtagat cgacttggcc cagtcgtggt ccaccacctc cgtcacctcg acgcccttgg
    10381 cgagcagctc gtggcgcttc tcgtccagcg ccgcctcgga gccggcctcg aacgcgaaat
    10441 ggtagaacac acccggcacc ccgagccccc ggttgatccc cgcgtcgtac tccgccggga
    10501 tgcccggcac gccgctcgcc tccatgaagg cgatgagctg atcgtgcccg gtatcgaaga
    10561 agatgtggcg gatctgtccg ccttccttga ccttgaggat gtcgcagcgc accgccttga
    10621 acccgagcac ccgctcgtag aagtcgcgcg tccggtccag atccagcgtc gagaggccga
    10681 tgtgggagaa ccctctgggg gccatgttgt gtcctccttg gcggcagtat acccgccgtc
    10741 acggcgactg agccggctcg cttcccgacg ggcgcgccgg cgggcgggtc aggctcggcc
    10801 ggcactcgta cacccggtcg ccgaccatct gtagcagcgt gtcgtcggcc ccgatccggc
    10861 gcaggaacgc gacggccctg gcgacccggc cctgccgcgc cgcgacggcc tgatcgcagg
    10921 cctccctcga ggcatacgtg cggctcccgg gcacccagtc gagccggtcg agcgtcgtgc
    10981 tccgccagat gacgtacccc tggtccgccg ccgccccgtc caccgccacc accgcgatgg
    11041 tccctatcaa gagcgccggg ccccacctgc cggtcgccat ggccgtgccc tccgtgaggc
    11101 ggtggtagca cactcccctg tatccttgga aggagggaag cgagacgtga tcacacgccg
    11161 ggagctcgag cgttggctac tgcgcgaggg ggccgtcagg gtgaagcgcg ccgacgggca
    11221 caagcacttc accctgcgcg gccatcacgt cgtggtcctg ggacacggtc cccagacgct
    11281 cagtgccacg agcgtgtcgc tcgtgatgaa gcagctcgag caggccggct acaaccggga
    11341 gcagctcaga cgggagtggg ggagcgagcg aagctgacgc ggccccgacc cgcggcctac
    11401 ttcccggtga agaactcctt gatcgtccgg ccgctgaact tggcgccctc gaccaccgtg
    11461 ttgccgatgc ccttggcggt ttccttcacg cccgtgccga cattgccctg accgatctgc
    11521 cgggcaccct gctggacctg ctgggtcgcc tggtccacct tggtcttgtc ggcggcgccg
    11581 gcggtgccga acccgagagt cgcgaccgcg atcaatccgg aagtgacgaa caaccaacga
    11641 acgctctgtc tcatcacgca cctcctgtac tcttcatcct acaccgggcc gcccgcctca
    11701 cgccttgcgc ttgcgtcgct ccctgatctc ccgcaggacc cccgacaggc cggcgcggtc
    11761 cttccgcggc cccagcgtcg tcgaccgccg ggcgggcgtc gcgagcgagg agttgtggtg
    11821 gcacacgagg cagtactcgc cccgtaccgt ggcgcagtcc ttcagctcgt ggtcgcaccg
    11881 tcgctcgctc ctgcaggcga aggacatgga gaaggagtcg cgccctacgg ccggccgcgc
    11941 aggaagtgga cgatcagcat gacgacgccc gcgaccagga gcacgtggat caggcccccg
    12001 gtcaggtgga gcacgaacag gtcgagggcc cacagcacga ccagcacgac gaaggcgatc
    12061 aggaaattcg atttgaacac ggcgatctcc cctttcgagt cagctcccgc tccagatatt
    12121 gtccgcgact ttccggccgt cccgcttcgc ggtgtacgcg accgtcaagc tggtcccggg
    12181 gctgagcccg cccgcagtct gcttgctcgt gagaaagatc ttcgtgccgt tctcgagcac
    12241 gatgtggtcc tcgtggcatt cccgcaggac gccgacgatg accccacccg ccatgcgaat
    12301 cccctgtccg gaccgtgaga gagagcgcaa gaccagattc cacgggatcg caacgccggg
    12361 cgcgtcgaac caggagagcc cgcaggaggt agggaaactg tagcacggaa cgagggcccg
    12421 cggtcgcgcc gtcttgattc gcgcgccgcg cgcaagtagt ctcggcgtca tgcgcatacc
    12481 ctccgaccgt cttcgccggc tcaccgctac catcctcgcc cgcggcggca gcgagcccgc
    12541 cgaggccgac ctggtcgccg cccacctcgt cgacgccaat ctcgcgggcc acgacagcca
    12601 cggcgtcggc ctcatcccgc actacgtgcg ccacctgaag gagggcctcg tcgtccccaa
    12661 cacctccgtg aaggcggtga aggacgacgg ggccttcctc atgttcgacg gccagcgcgg
    12721 gttcgggcgc cgcgtggccg gcgaggcgat ggccgccgcg atggcgcgcg cccgggacgc
    12781 gggcgtggtc gtcgtgacgc tcgcgaactc gcaccacgtc gggcgggtcg gcgcctacgg
    12841 cgagctggcc agcgcggccg ggctcgtctc gatccacttc gtgaacgtgg tggatcatac
    12901 cgggctggtg gcgcccttcc gcgggagcga ctcgcgcttc tccaccaacc cggtctgcat
    12961 cggcatgccc ggcaccgcca cccagccgcc gcttctgctc gacatggcca ccagcggcgt
    13021 ggcgatgggg aaggtggccg tcgcgaagcg agcgggcaag aagctgcccg acggcaacct
    13081 gatcgacgcg ctgggcgcgc ccaccaacga cccggaggtg atgtaccagg agccgcgcgg
    13141 ctccctgctg cccttcggcg cgcacaaggg gtacgcgctg gccgtcctca ccgagctgct
    13201 cgccggcgcc atgtcgggcg gcgggacgat ccagccggag aacccgcgcc gcgggggcgt
    13261 cgtcaacaac atgttcagcg tcctcatcga tccggcgcgg ctggccggcg tggactggat
    13321 gcggcgcgag atcgacggct tcgtcggcta cgtgaagggc tcgccgccag ccgatccggc
    13381 cagcccggtg ctggtgccgg gtgaccccga gcggctctcg cgcgaggcgc ggcagcgcgc
    13441 gggcatcccg ctggacgcga ccacgtggga ggagatggtg accgcgggcg cggtgatggg
    13501 cgtgacgcgc gcgcaggccg aggcgatcgc cgcgcccacc gcggactgag ccgggcccgc
    13561 cctcgatgat ccgcatcctc accggcgcca ccctgatcga cggcaccggc gccaccccgg
    13621 tgccggacgc cgcggtgatc atgaacggcg accgcatcac cgccgccggg cctcgcgcgg
    13681 cgctgtcctg gcccgcgggc gccgagatcg tcgacgtgcg cggccgcacg gtcatccccg
    13741 gcctcatcga cgcgcacgat catctcgcct cgcacggcta cggcctggcc acccgctggg
    13801 gtctcgacga gcccgcgagc accgcgcacc tccgcaccgc gcgcgtgctc gcggagaccc
    13861 tcgccatggg ctacaccacc gtgcgcgacg gcggcgggct cgacgcgggc ttcaagctcg
    13921 ccatcgagca ggggctgatc ccggggccgc gcctggtgct cgccgtgcag atcatctcgc
    13981 cgaccggcgg catcggcgat cgcgtgagcc cctcgggcca cgactgctgc gccgcgtacg
    14041 atcccctgct gccggccagc gtggccaacg ggcccgatgc ggtgcgcgac gtggttcgca
    14101 ccatggtccg cgcgggcgcc ggcgtgatca agaccgcgac caccggcggc gccagctcgc
    14161 gcgccgggca cggcccgctc gacccggcct tcacgctcgg cgagatggac gcgctggtgg
    14221 cggagtcgca cgcgctcggg cggcgcgtga tgtgccacgc cctcggcggc cccgggctgc
    14281 gcaccgcgct cgaggccggc gtcgatacga tcgagcacgg gtgctatctg gacgaggatc
    14341 ccaccctgct cggccagatg gccgtacagg gcaccttctt cgtgcccacg ctcaccgtct
    14401 acgtgtacca ccgcgagagc ctgtccccgc acgtccggga gcgcgccctc gcgctctacc
    14461 cgcaccacgt ggccagcgtg cgccgggcgc tcgagctggg cgtgccgatc gcggcgggca
    14521 ccgacgcggg cggccacggc catccgagga acgcgctcga gctcaagtac ctggtcgagg
    14581 cgggcctgtc gccgatgcag gcgctgcgcg cggccacgca gtgggccgcg gcgtgcctgg
    14641 ggctcgagcg cgaggtcggc acgatcgaga aggggcgcct ggccgacctg gtggtggtca
    14701 acggcaaccc gctcgacgac atcacggtgc tgctggatcc cgcgcgcgtc gagctggtgt
    14761 ggaagggcgg ggtgatctgc gcggaccggc ggagcccgcg ctagcgccgc tcccgatgga
    14821 cccccagcag ctcctcgacg agctggccca ggccgcgaac cggctcggcg tcgaggtccg
    14881 gacggagccc ttcgagacgc ccgcgacgat gggcggcggc ctctgcatcg tgcggggcga
    14941 gagcctcgtg ctgatcgacc agcgcgcgcc gctgccggat cgcctccgcg cgctcgcccg
    15001 ggccctggtc gagctccgga gcgagaccgt ctacatggcg ccggaggccc gggagctgat
    15061 cgaagccttg cgagctccgg ccgtaccggc cgccccccgg cagacctcgt gagcggtcag
    15121 cgcccccgcc ggcgacgcct ccgcgcgacg gttcgcccgg tatgacccgg agtgcctttt
    15181 ctcaggctct ccacgaatcg taggcggcag tcctcgctgc agaaccggct cactccctcg
    15241 gccaggtcgg cctgacacgt ctcgcacttg agtgtgacca tgctcacact ctagagcgcg
    15301 cgcgcccgcc cgggtattgg accttggtcc agcgcgggga ccgcgccgga ccacgccccc
    15361 gaccgcctag ccggacgcct tcttgaagaa ctccccgatc gtctcgccgc tgaacttggc
    15421 gccctcgacg atcgtgtggc cgatgccgat gaagaactcc ttgaagcccg gaccgagctc
    15481 cccctggccg atctgcttgg cgccccgctc gacccggctc gtcgcctggt ccaccttgga
    15541 ccggtcggcg gcgccggaca cgccgaagga cagggccacg accatgaggc atgccgggat
    15601 gatccacagt ccgcgaagtc tccgcatcat gacgcacctc ctgtagtggg gttagcccgc
    15661 agtctcaatg ctagcaccag ccgcgggcgg gcgctcgcga tcgcaggcgc gatgtgccac
    15721 aatgcaagtc gcgcgcggga gaaccaggtg ccagacaccg acgacagccc ctcaccctcg
    15781 atcaagcgcc ggctcgccgc catcctggcc gccgacatcg ccggctacag ccggctcatg
    15841 ggagaggacg aggccgcgac ggtccgcgac ctcaaggagc atcaggcagt gatcctgccg
    15901 ctggtcgtcc gttacggcgg ccgcatcatc gacaccgcgg gcgacggcat cctggccgag
    15961 ttccccagcg tgatcaacgc gaccgagtgc gcggtcgaga tccagaccgt catggcgacg
    16021 cgcaacgagg cggtgccgga ggcgcgccgc atgcggttcc gtatcggcat caacctgggg
    16081 gacgtcatcc acgacgacac ccggatctac ggcgacggca tcaacgtcgc ggcgaggctc
    16141 gagggcatcg cggagcccgg cggtatctgc atctcccgcc aggtgttcga tcaggtgagc
    16201 cgcgcgctca aggcggattt ccaggcgctc ggcccgcgca ccttcaagaa catcgcgcgg
    16261 ccggtggacg tcttcgccat cgcgcccgcg ggcggcgacg gcgggcccgg cgtgaccggc
    16321 gccaatgccg tcgacctccg gcaggaggtt cgcttctgca ccgcgcccga cggagttcag
    16381 ctcgcctact cgatgatcgg ccagggcccc ccgctgatga agaccggcaa ctggatgact
    16441 catctggagt tcgatctcga gagcccgatc tggcggccgc tctatcgaga gctggcgacg
    16501 gaccacacgc tcatccgcta cgacgcgcgc ggcaatgggc tgtcggaccg ggccgtcgac
    16561 gagatctcgt tcgacgcctt cgtgcgcgat ctggaggcgg tggtggacgc cgcgggaatc
    16621 acgcggttcg cgctgctcgg gatctcccag ggctgcgcgg tctcgatcgc atacgcggtg
    16681 cgacaccccg agcgggtctc gcgtctgatt ctctacggcg gctacgcgct gggctggagc
    16741 aagcgcgccc ggagcgcggc ccagaaggaa gaggacgaag ccgtgctcac gctgatgcgc
    16801 gtgggctggg gcaaggagaa tccggccttc cgccaggtgt tcacctcgca gttcgtgccc
    16861 ggcgcgacca aggagcagtc cgactggttc aacgagctgc agcggatcac ggtctcgggg
    16921 gacaccgcgg cccggatctt cacggcaacc ggcgagaccg acgtgaccgc gctgctcccg
    16981 cgggtgagcg tgccgacgct ggtcctgcac gcgcgcgacg acgcccgcgt ccccttcgaa
    17041 gccgggcgcc gcatggcggc cggcattccg ggcgcccggt tcgtggcgtt gacggggcgc
    17101 aaccacgtgt tcctcgagtc cgagccggcc ttcgcccagt tcctcgagca cgcccgggcg
    17161 ttcctggccg gctaggccct gtcctcccgg gacgctcaga cgcccaggta gtactgcttc
    17221 accagctcgt tctcccgcag ctcccgcgtg ctgcggccct cgaactcgat cttgccgtgc
    17281 acgatgatgt agccgcggtc ggcgatcttg gtggcctggt tgaagttctg ctcggccatg
    17341 agcaccgtga gctggcgttg ctccttcatc tcgcggatct tgccgatcac ccggctgacc
    17401 agaatagggg ccagtcccac cgagggctcg tcgaccagga gaatccgcgg ggaagacatg
    17461 agcgcgcggg ccaccgccag catctgctgc tcgccgcccg acatgctgcc cgcgagctgg
    17521 cgccggcggc cggccaggac cgggaacgcc tcgaacgcga acgcgaggtt ggtcgcgatg
    17581 tcggcgcggg cgcccgcgcg gtaggccccc agcaggaggt tctcctcgac ggacagcttc
    17641 gggaagagcc ggcggccctc gggcaccagc gcgatcccca tgcccacgat ctcctcggtg
    17701 gacttgcggg tgagatcgac gcgggtgccg tcggtctcca gaaagatctc gccggcctcg
    17761 ggcctcacca tgcccatgat gcacttgatg agcgtgctct
//
LOCUS       DASUFA010000268         4584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1102138, whole genome shotgun sequence.
ACCESSION   DASUFA010000268 DASUFA010000000
VERSION     DASUFA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102138"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..693
                     /locus_tag="VFX87_09975"
     CDS             82..693
                     /locus_tag="VFX87_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX4995288.1"
                     /translation="MLPPFAELEATLAAEPSARNRAIIAMALYGGLRAEEIQKIRRGS
                     FVADQGLIGFVGKGRKQRSVALPPQALRLITAYLAGNGKLATPSDPLIRKEDGSGDGL
                     SYHVINRVVTRWTKRHLGVRLTPHKLRHAYGRQCVDLGVDIRVIAEALGHESLESTKI
                     YTQVSFQRTRKIAELFCSPGESTTPGQNGQQTQSVPAGLNFQK"
     gene            complement(657..2063)
                     /locus_tag="VFX87_09980"
     CDS             complement(657..2063)
                     /locus_tag="VFX87_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX4995289.1"
                     /translation="MIGIVSAGGYVPRYRLSAKTLASVWGSGGSGERAVANYDEDSLT
                     MAAEAALNALHGRGVSRIGACFFASTTPPYLEKSSATLLAAVADLGPSVLTADFGGSL
                     RCGTTALRLALDAVKAGTATEALVAAADIRPVAPGTPEEIQIGDGAGALLVGSADVIA
                     SFEGGASVSREFTDVWRGPGDRYLTVLPDMTFVKAHGLDKHIAEAVEGALHATGRKRE
                     DIARIVLYGPDARTHAALVKQLRLPERAMPKESVIGRTGNTGSASCLLGLAAALEECR
                     PGDQVLVVSYGSGAEALLFQCTDRLAQAAMHRPVSAQLAHGKPLTQYGRFLSFRRHVE
                     TEVMRAFSSVPTMVREERQDLRLYGQKCADCGAVSYPRRHLCWQCSSKSLAEYRISRR
                     GKIFTFTRDHLVPSPDPPTVMVSADLEGGGRFYGQLTDCDPAAVTFEQPVELCFRRIH
                     EGEGIINYFWKFRPAGTD"
     gene            complement(2060..3838)
                     /locus_tag="VFX87_09985"
     CDS             complement(2060..3838)
                     /locus_tag="VFX87_09985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX4995290.1"
                     /translation="MNPLYHDAIERRLAVPLHEGPDKTTTLAEAIRRHVHAGDTVYLG
                     SAHGRPNPLVRELVRQWWGQRPAFTLAVVGLGSPWTALIHGGLVRRVITTFMGEGYPF
                     PTPQALISRAVLDGRLEVSNWSMLTFPLRLLAGAMGVPFLPTRSLLGSSMEEDNTRSG
                     EFMTADDPFGAEGRVGYARALVPDVALLHAWAADRAGNVIIAPPMNENMYGAMAAKNG
                     AVVTVEKIVSTDFVRRHANLVRLPGQYVRAIVETPHGNHPGGMYGMNVPELEGYAEDI
                     EWILECRRAFRKPETADAWIKEWMLDLPDHAAYLAKLGYARIMEIKGRAHTDAWVSEL
                     ETLSPKLPLSATASPSEWMVVSAARMLAEKVQARGYKTFLAGVGNSNLAAWLAAYQLK
                     QAGADVELMAETGMVGYLPRPAEPFVFSFRNFPSCKMLTDILHVMGIFMGGRHNQCLG
                     SLAAGQIDKHGNINSTIMPGVTYITGSGGANDITSSAREVVVCLEQSPRRFVDKVPYI
                     TAPGRTVRTVVSDRGVYEKAGEHGELRLTGLFGDAPEADLVRGARGACGWDLQVAPAL
                     RRFEAPSADELALVRLFDPRRYFLGA"
     gene            complement(4042..4143)
                     /locus_tag="VFX87_09990"
                     /pseudo
     CDS             complement(4042..4143)
                     /locus_tag="VFX87_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
     gene            complement(4240..>4584)
                     /locus_tag="VFX87_09995"
     CDS             complement(4240..>4584)
                     /locus_tag="VFX87_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX4995291.1"
                     /translation="TYHDACNLGRYNDVYDEPRRVLASLTQGPIVEMELSRSRGFCCG
                     GGGGRAWVEENEGRRVNQLRVEQAMEVKPDILASACPFCLTMFEDGVKAKEVGDKIKT
                     RDIAELLAERLE"
BASE COUNT          723 a         1576 c         1641 g          644 t
ORIGIN      
        1 ttcggtccga gtagagttcg agtcaactcg agctgaagct gggcccgaaa tggaccaggc
       61 cggctcaagc gcgtcgttcc ggtgttgcca ccgttcgcgg agctcgaggc cacactggcg
      121 gccgagccga gcgcaaggaa ccgcgccatc atcgccatgg ccctctacgg gggccttcga
      181 gcggaggaga tccagaagat caggcgaggc agcttcgtgg ctgaccaggg cctgataggc
      241 ttcgtaggca agggccgcaa gcagcgttcg gtcgcgctgc ccccgcaggc gctcaggctc
      301 atcacggcct acctggccgg caacggcaag ctcgccacgc ccagcgaccc gctgatccgc
      361 aaggaggacg gcagcggcga cggtctcagc taccacgtga tcaatcgcgt ggtcacccga
      421 tggaccaagc gccacctcgg tgtgcgcctc acgcctcaca agctccgcca cgcctacggc
      481 cggcagtgcg tcgacctcgg cgtggacatc cgcgtcatcg ccgaagcgct cggccacgag
      541 tcgctggagt cgaccaagat ctacacgcag gtgtccttcc agcggacccg aaagatcgcc
      601 gagttatttt gctcccccgg ggagtcaact actccggggc aaaacggcca gcaaactcaa
      661 tcagttccag cgggtctgaa cttccagaag tagttgatga tcccctcgcc ctcgtggatg
      721 cggcggaagc agagctcgac gggctgctcg aaggtgaccg cggcggggtc gcagtcggtg
      781 agctggccgt agaagcggcc gcccccctcc aagtcggccg acaccatcac ggtgggcggg
      841 tccgggctcg ggaccagatg atcgcgggtg aaggtgaaga tcttgccgcg gcgggagatg
      901 cggtactcgg ccaggctctt cgacgagcac tgccagcaga ggtgccggcg cgggtacgag
      961 acggcgccgc agtcggcgca cttctggccg tagagacgca ggtcctggcg ctcctcgcgc
     1021 accatggtgg gcacgctcga gaaggcgcgc atgacctcgg tctccacgtg gcggcggaag
     1081 gagaggaacc ggccgtactg cgtgagcggc ttgccgtggg cgagctgggc cgagaccggg
     1141 cggtgcatgg ccgcctgcgc cagccggtcc gtgcactgga acagcagggc ctcggcgccg
     1201 ctaccatagg agacgacgag cacctggtcg ccgggccggc actcctccag tgcggcggcc
     1261 agtcccagca ggcacgaggc cgagccggta ttgccggtgc gaccaatcac cgactccttc
     1321 ggcatcgcgc gctcgggcag gcggagctgc ttgaccagag ccgcgtgggt gcgggcgtcc
     1381 ggaccataga ggacgatcct ggcgatgtcc tcgcgcttgc gcccggtggc gtgcagggcc
     1441 ccctcgaccg cctccgcgat gtgcttgtcg aggccgtgcg ccttcacgaa ggtcatgtcg
     1501 ggcagcacgg tgaggtagcg gtcgcccggg ccgcgccaga catcggtgaa ctcgcggctc
     1561 acgctggcgc cgccctcgaa ggaggcgatg acgtcggcgc tgccgaccag gagcgcgccc
     1621 gcgccatcgc cgatctggat ctcctccggc gtgcccgggg ccacgggtcg gatatcggcc
     1681 gccgccacca gcgcctcggt cgcggtgccg gccttgactg cgtccagcgc cagccggagc
     1741 gcggtcgtgc cgcagcgaag cgacccgccg aagtcggcgg tgaggaccga cgggccgagg
     1801 tccgcgaccg ccgccagcag ggtggcgctc gacttctcga gatacggcgg cgtggtggag
     1861 gcgaagaagc aggcgccgat gcgggagacg ccgcggccgt ggagcgcgtt gagcgcggcc
     1921 tcggccgcca tggtgaggct gtcctcgtcg tagttggcga cggcgcgctc tcccgagcct
     1981 ccgctacccc acaccgacgc gagggtcttg gcggagagtc ggtagcgcgg gacatagccg
     2041 cctgcggaga cgatcccgat catgcgccca ggaaataccg gcgcgggtcg aagaggcgga
     2101 ccagggccag ctcgtccgcc gacggggcct cgaagcggcg cagcgccggc gccacctgga
     2161 ggtcccagcc gcaggcgccg cgggccccgc gaaccaggtc ggcttccggc gcgtcgccga
     2221 agaggccggt caaccgaagc tcgccgtgct cgcccgcctt ctcgtacacg cctcggtccg
     2281 acaccacggt gcgcaccgtg cgtcccggcg cggtgatgta cggcaccttg tccacgaagc
     2341 gccggggtga ctgctcgagg cagaccacca cctcgcgggc ggaggaggtg atgtcgttgg
     2401 cgccgcccga gccggtgatg taggtgacgc cgggcatgat cgtcgagttg atgttgccgt
     2461 gcttgtcgat ctggccggcc gcgagcgagc ccaggcactg gttgtggcgg ccgcccatga
     2521 agatgcccat gacgtgcagg atgtcggtga gcatcttgca ggacgggaaa ttgcggaacg
     2581 agaacacgaa gggctcggcg ggccgcggca gataacccac catgccggtc tcggccatca
     2641 gctccacgtc ggcgcccgcc tgcttgagct ggtacgcggc cagccaggcg gccaggttgg
     2701 agttgcccac gccggccagg aaggtcttgt acccgcgcgc ctgcaccttc tcggcgagca
     2761 tccgcgcggc cgagaccacc atccactccg acggcgacgc ggtggcgctc aggggcagct
     2821 tgggcgagag cgtctcgagc tccgagaccc aggcgtcggt gtgggcgcgc cccttgatct
     2881 ccatgatgcg ggcatagccg agcttggcca ggtaggccgc gtgatcgggg aggtcgagca
     2941 tccactcctt gatccacgcg tccgcggtct ccggcttgcg gaaggcccga cggcactcga
     3001 ggatccactc gatgtcctcc gcgtagccct cgagctccgg cacgttcatg ccgtacatgc
     3061 cgcccggatg gttgccgtgc ggcgtctcca cgatcgcgcg cacgtactgc ccgggcaggc
     3121 gcaccaggtt ggcgtggcga cggacgaagt cggtggagac gatcttctcc accgtgacga
     3181 ccgcgccgtt cttcgcggcc atcgccccgt acatgttctc gttcatcggc ggcgcgatga
     3241 tcacgttgcc ggcccggtcg gcggcccagg cgtgaagcag cgccacgtcg gggaccagcg
     3301 cccgcgcgta gcccacgcgg ccctcggccc cgaagggatc gtcggcggtc atgaactcac
     3361 cactccgcgt gttgtcctcc tccatgctcg agcccagcag cgagcgcgtg gggaggaacg
     3421 gcacgcccat ggcccccgcc agcaggcgca gcgggaaggt cagcatggac cagttcgaga
     3481 cctcgagccg gccgtcgagg acggctctcg agatgagggc ctgcggggtc gggaacggat
     3541 agccttcgcc catgaacgtg gtgatgaccc ggcgcacgag gcccccgtgg atcagggcgg
     3601 tccagggcga gcccaggccg accaccgcga gcgtgaaggc ggggcgctgg ccccaccact
     3661 ggcgcaccag ctcgcggacg agcgggttgg gccggccgtg ggccgagccg agatagaccg
     3721 tgtcgcccgc gtggacgtgg cggcgaatcg cctcggcgag cgtggtggtc ttgtcggggc
     3781 cttcgtgcag cggcaccgcg aggcggcgct cgatcgcgtc gtgatagagc gggttcatcg
     3841 agacccctcc ccctgcccgc tcccccgagg ggagagggcg gaagaaccga atagacgcgc
     3901 cttgaggcgg ttggggaccc agcgggccag gcggtgggcg accgcggcgg tacgctccag
     3961 ggagaccgcc gagcccggcc agtagcgaag cagcaggggg cgatccacga aggtcagcac
     4021 accgtccacc accaccgcgg ccaggatcac gtcgtcgagg tagccgagcc acgggatgaa
     4081 gtcgggcacc aggtcgatcg ggctcaccag gtagacggcc agcgcggcga gcacgacctt
     4141 ggcggcggtc gggacctggc ggtccccggc cagcgagatc agcatgcggc cgagccgcgg
     4201 cagggcgagg agcaagtcct tcacggtccc ggccccgggc tactccaggc gttcggcgag
     4261 gagctcggcg atgtcgcggg tcttgatctt gtcgccgacc tccttcgcct tcaccccgtc
     4321 ctcgaacatg gtcaggcaga acgggcaggc ggaagccagg atgtcgggct tcacctccat
     4381 cgcctgctct acccggagct ggttcacccg ccgcccctcg ttctcctcca cccacgcacg
     4441 cccgccgccg ccgccgcagc agaacccgcg ggaccgcgac agctccatct cgacgatcgg
     4501 cccctgcgtc aacgaggcca ggacgcgccg cggctcgtcg tacacgtcgt tgtagcggcc
     4561 gaggttgcag gcgtcgtggt aggt
//
LOCUS       DASUFA010000269         8554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_740406, whole genome shotgun sequence.
ACCESSION   DASUFA010000269 DASUFA010000000
VERSION     DASUFA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740406"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFX87_10000"
     CDS             complement(<1..671)
                     /locus_tag="VFX87_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX4995292.1"
                     /translation="MSETTPAIQDYLGAIYDLAGTDKPVIGARLARHMHVSAPSITEA
                     LRRMQKDGYIRVGGRKEIRLTTKGLGIAETMARRHRLIERWLTDVLGLDWSRAHDEAH
                     RLEHALSPVVEERLAETLGMPSTCPHGNPIPGMPAPEAHSPIPLSQAPAGHPFVVERI
                     TEEAEADRQLLRFLWESGIRPGSRIVVSEIAPYAGTVSIVLEGRTVTMGISAANKIWV
                     YDPLLP"
     gene            756..1919
                     /locus_tag="VFX87_10005"
     CDS             756..1919
                     /locus_tag="VFX87_10005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4995293.1"
                     /translation="MDFALTESQELIKKEVGALARTFSLDYWLDKDDRAEYPSDFVKA
                     FADNGWLGITIPEAYGGAGLGVTEAALMLHEICASGAGTSGCSPIHFYVFPPTPLVKY
                     GTEAMKRKYLPRIVSGDIVMAFGVTEPNAGTDTSRIQTRAVKQGDRWIVNGRKVWTTN
                     AQHANKILLLARTADRDPARPLKGMTLFFADFDRTAITVRLIHKLGRAAVDSNEIFID
                     NLEIPDADVVGTPGEGFYHLLDSLNPERIVIGIEAVGIGRAALDRAVQYAKERVVFDR
                     PIGKNQAIAHPLAHAMAQLDAAEMMCLKAAWLFDHGKPCGREANAAKMLAAEAGFNAC
                     DAALQTFGGFGYAKEFHVERLWREIRLYKIAPVSQQMALNYLAEHVLGLPRSY"
     gene            1924..2346
                     /locus_tag="VFX87_10010"
     CDS             1924..2346
                     /locus_tag="VFX87_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995294.1"
                     /translation="MELLLTTHDTGYERPDGPTIAKVLASLDGGRNVLATLGTTDATY
                     LQASGSVQTGFGLDLQEGSLKRRFRTRDRALPLPWVTEAFQRYAAGDLSWRGAVEWEQ
                     EHVPVVRESWMNSWAAYIALLVAVAAAMWFFHAWRTSP"
     gene            complement(2355..3059)
                     /locus_tag="VFX87_10015"
     CDS             complement(2355..3059)
                     /locus_tag="VFX87_10015"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4995295.1"
                     /translation="MTPERFAQGMTFDEYVKFTGSPRNLAREGFDIRRFSVVRPRLDW
                     SAFLHKRHAGARLTPEQIAAVQWLAAQPGGPAKVAVISEDWSSDCRRDVPYLARLAKA
                     GGLELRVFTRDGDTMLLKGLPDPASGGNADLVQAYAHEKNGERWASVPVAVFFTEDFV
                     ELYRYVEYPAIYRKERVFGHLRAARPGETPEAAKARAGAEIGALLESPFFDVWAQAAI
                     AEILSALHERLLIPPA"
     gene            complement(3088..4248)
                     /locus_tag="VFX87_10020"
     CDS             complement(3088..4248)
                     /locus_tag="VFX87_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX4995296.1"
                     /translation="MKRSDRRILTTHVGSLPRPPALRDLLVRRDRGEPVDAAALARET
                     DAAVLDVVMRQLSVGIDVGNNGEQPRAGFSTYVAGRMRGFGGTSRRLPARDLIDFPDY
                     AEMLARRRRDAARIADAPQAIAEVEYADLGPAARECDDFLRHTDGRAPGFAERFMTAA
                     SPGVIATILLSAHYRSHEQYVMALAREMRKEYELIHARGLLLQVDCPDLAMERVRFFQ
                     NDSLEAFQRSVELHVEAINQATAGIPPDRIRLHVCWGNYDGPHTHDVALEAVLPLVYR
                     ARVGALSLPLANPRHQHEYRVFKRYPLPDSMLLLPGVIDTTTNYVEHPEVVADRIAAA
                     AEAVGDPTRVIASTDCGFGTFAGSEMVAHSVVWAKLGALTEGAAIASRRLFT"
     gene            complement(4250..5143)
                     /locus_tag="VFX87_10025"
     CDS             complement(4250..5143)
                     /locus_tag="VFX87_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX4995297.1"
                     /translation="MHGRHTHGSRHKRHLLAVFGLTSAFLVVEAAVGLWTGSLALLAD
                     AVHMLVDAGGVLLSLLAVWFAERPATPAKTYGYYRVEILAALVNGVVLCVMAVAILVT
                     TYERLWQPPHVPGGPILVVATLGLLVNLAGMWLLHGGAAESLNVRSAYLEVLGDAVSS
                     ALVIVAGLVIILTGWTLADVIAGALIALFILPRTWALLRQAVNVLLEGAPPHLDVTEI
                     EEALRSAPAVRRVHDLHVWTLTSGREAMSAHVVVEAGAPADRILEALHLVLHSRFGID
                     HTTIQIETEPAPLIQITPRAG"
     gene            complement(5152..5667)
                     /locus_tag="VFX87_10030"
     CDS             complement(5152..5667)
                     /locus_tag="VFX87_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX4995298.1"
                     /translation="MIPSRVAILAALLLVLTVPAAMAQGPAKGPAPNPECPRWREAFA
                     GMPIRMVGVQAGARSIALRVKAADTGERQAGGFQCATPEEIKRHLILFDFGREIVTQF
                     HMQNVPAALDIAFVKEDGRIFAILKMDPSPTELYGPLGSFRYAIEAHAGFFASQGIRQ
                     GEARLVLPPAR"
     gene            complement(5664..6491)
                     /locus_tag="VFX87_10035"
     CDS             complement(5664..6491)
                     /locus_tag="VFX87_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX4995299.1"
                     /translation="MPEFLQYGFMHRAFAAGVVTALICPAIGVFLIPRRLSLIADTLA
                     HVALAGVALGLVLGISPVLGALVVALAGAVGIERLRARGALQGDAALAVFLSGGFALA
                     VVLISLARGFNADLFAILFGSILTVTSGDVWLIVALGAVVTVAVGGFYKQLLAITLHE
                     DLARTSGVPVGALNLLLTVLTALTTVVAMRMVGVLLVSAMIVIPALTGFALGRSFHTA
                     TAIAIGIALLSMTSGLIAAYYLRLAAGGAVVLAALLIFAAASLARRRWPATAREAHP"
     gene            complement(6496..7239)
                     /locus_tag="VFX87_10040"
     CDS             complement(6496..7239)
                     /locus_tag="VFX87_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX4995300.1"
                     /translation="MRSFVELEDVDFAYGAVPVLERINLTVEPGDFLGIIGPNGSGKT
                     TLLRITLGLLPPQHGTVRLFGHPPASFRQWGRLGYVPQKATLDPALPVTVHEVVATGL
                     LPSLGLFGRIGGTERKRIAEVLGQVGMDAHAGARIGALSPGQQQRVLIARALVSKPEL
                     LILDEPTGGVDPEAQTSFYALLHHLNRERDVTLILVSHDIGVVAKEVTKLACLNRRLV
                     FHGRPGDFLSDAALTALYGPAVRVVSHDH"
     gene            complement(7239..7892)
                     /locus_tag="VFX87_10045"
     CDS             complement(7239..7892)
                     /locus_tag="VFX87_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX4995301.1"
                     /translation="MDAATVARWRALCLVLLGAFLGMSLVVAAVGLLPGDVPLHDEIL
                     KGRGTLLHAVARWVDYGGKWIVLGPAIALLFVWSRAARRHWWLWCALMPIGGAVEQAF
                     KFLVGRPRPRGVNWGFPSGHVTAAATFAVILLYIMTRERVSPAARGILTALAFALVIA
                     VGFARVILNAHWPTDVLGGVLLGAGCAAAGAWWDARHPETTQPARPRAVNDALEIGG"
     gene            complement(8343..>8554)
                     /locus_tag="VFX87_10050"
     CDS             complement(8343..>8554)
                     /locus_tag="VFX87_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995302.1"
                     /translation="RRGPPAAGVTMTEPPPLPTRWLNTYTGTLGLVGVLFVISPLLLR
                     PGGSAANLVMVGLGKLGQWPGARGS"
BASE COUNT         1317 a         2925 c         3160 g         1152 t
ORIGIN      
        1 ggcagcagcg ggtcgtagac ccagatcttg ttggccgcgc tgatccccat ggtgacggtg
       61 cggccctcga gcacgatcga gacggtgccc gcgtagggcg cgatctcgct caccacgatg
      121 cggctgcccg gccggatccc gctctcccac aggaagcgca ggagctggcg gtcggcctcg
      181 gcctcctcgg tgatgcgctc gaccacgaac gggtggcccg cgggggcctg gctgagcggg
      241 atcgggctgt gagcctcggg ggcgggcatg cccgggatcg ggttgccgtg cgggcaggtg
      301 ctcggcatgc cgagggtctc ggcgagccgc tcctccacca ccggagagag ggcgtgctcg
      361 aggcggtgcg cctcgtcgtg cgcgcgcgac cagtcgaggc cgagcacgtc ggtcagccag
      421 cgctcgatca ggcgatgccg gcgcgccatg gtctcggcga tgccgagccc cttggtggtg
      481 agccggatct ccttgcggcc gccgacgcgg atgtagccgt ccttctgcat gcggcgcagc
      541 gcctcggtga tggagggcgc ggagacgtgc atgtgccgcg cgagccgcgc gccgatcacc
      601 ggcttgtcgg tgccggccag atcgtagatc gcgcccaggt agtcctggat cgccggagtg
      661 gtctcgctca tggccgccag cataccgacc ggcgcggcgg cgggcaagta cccgggccgc
      721 cgtgatactc ttgcccgacc aggaggcccg gctcaatgga cttcgcgctc acggaatcgc
      781 aggagctgat caagaaggag gtgggggcgc tggcccgcac gttctccctc gactactggc
      841 tcgacaagga cgatcgcgcc gagtacccga gcgacttcgt gaaggccttc gccgacaacg
      901 gctggctcgg catcacgatc ccggaagcgt acggcggggc cgggctgggc gtcaccgagg
      961 ccgcgctgat gctccacgag atctgcgcct cgggcgcggg cacctccggc tgctctccga
     1021 tccatttcta cgtcttcccg cccaccccgc tcgtcaagta cggtaccgag gcgatgaagc
     1081 ggaagtacct gccgcggatc gtgagcggcg acatcgtgat ggccttcggg gtgaccgagc
     1141 ccaacgcggg caccgatacg tcgcgcatcc agacgcgcgc cgtgaagcag ggcgatcgct
     1201 ggatcgtcaa cggccggaaa gtctggacca ccaatgccca gcacgccaac aagatcctcc
     1261 tgctcgcgcg caccgccgac cgcgatccgg cgcgtccgct caaaggaatg acgctcttct
     1321 tcgccgactt cgatcgcacc gcgatcacgg tgcgcctgat ccacaagctg ggccgggccg
     1381 ccgtcgactc caacgagatc ttcatcgaca acctggagat ccccgacgcg gacgtggtgg
     1441 gcacgccggg cgagggcttc taccacctgc tcgattcgct caaccccgag cgcatcgtga
     1501 tcggcatcga ggcggtcggc atcgggcgcg ccgccctcga ccgcgcggtg cagtacgcca
     1561 aggagcgggt cgtcttcgac cggccgatcg ggaagaacca ggccatcgcg cacccgctcg
     1621 cccacgcgat ggcccagctc gacgcggccg agatgatgtg cctcaaggcc gcgtggctct
     1681 tcgaccacgg caagccctgc gggcgcgagg ccaacgcggc caagatgctc gcggcggagg
     1741 ccgggttcaa tgcctgcgac gcggcgctcc agaccttcgg cggcttcggc tacgccaagg
     1801 agttccacgt ggagcggctc tggcgggaga tccggctcta caagatcgcg ccggtctctc
     1861 agcagatggc gctgaactac ctggccgagc acgtgctcgg gctgccgcgc tcctactagg
     1921 gcgatggaac tgctcctcac gacccacgac accggatacg agcggcccga cgggccgacc
     1981 atcgccaagg tgctggcgtc gctggacggg ggccgcaacg tgctggcgac cctcggcacc
     2041 accgacgcga cgtatctgca ggcgagcggg agcgtgcaga ccggcttcgg cctcgacctg
     2101 caggagggct ccctgaagcg gcgcttccgc acccgggatc gcgcattgcc gctgccgtgg
     2161 gtcaccgagg cgtttcagcg ctacgcggcc ggcgatctct cctggcgcgg cgcggtggag
     2221 tgggagcagg agcacgtccc ggtcgtgcgc gagtcgtgga tgaacagctg ggccgcctac
     2281 atcgcgctcc tcgtggcggt cgccgccgcg atgtggttct tccacgcctg gcgcacctcg
     2341 ccgtaggcgc ccgtctaggc gggtgggatc aggaggcgct cgtgcagcgc gctgaggatc
     2401 tcggcgatcg cggcctgcgc ccatacgtcg aagaagggcg actcgagcag ggcgccgatc
     2461 tcggcgccgg cgcgcgcctt cgccgcctcc ggggtctcgc cgggacgcgc cgcccgcagg
     2521 tggccgaaca cgcgctcctt gcggtagatc gccggatact cgacgtatcg gtatagctcg
     2581 acgaagtcct cggtgaagaa gaccgccacc ggcaccgacg cccaccgctc cccgttcttc
     2641 tcgtgggcgt aggcctgcac cagatccgcg ttgccgcccg aagccgggtc gggcaggccc
     2701 ttgagcagca tcgtgtcgcc gtcgcgggtg aagacgcgca gctcgaggcc gcccgccttg
     2761 gccagccgcg cgaggtaggg gacgtcccgc cggcagtcgg aggaccagtc ctcggagatc
     2821 accgcgacct tggcgggccc gcccggctgg gcggccagcc actggacggc cgcgatctgc
     2881 tcgggggtga gccgcgcccc ggcgtggcgc ttgtggagga aggcgctcca gtccagtcgc
     2941 ggacgcacca cgctgaaccg gcggatgtcg aagccttcgc gagcgaggtt tcggggggag
     3001 ccggtgaact tcacgtactc gtcgaacgtc atgccctggg cgaagcgctc cggtgtcatg
     3061 cgcaattcat cctctcgtgg gccaatctca ggtgaagagg cgacggctcg cgatggccgc
     3121 gccctcggtg agggcgccga gcttggccca caccacgctg tgggccacca tctcggagcc
     3181 cgcgaacgtg ccgaagccgc aatcggtgga ggcgatcacg cgcgtagggt cgcccaccgc
     3241 ctccgccgcc gcggcgatcc ggtcggccac cacctcggga tgctcgacat agttcgtggt
     3301 ggtgtcgatc accccgggca gcaggagcat ggagtcgggg agcgggtacc gcttgaacac
     3361 gcggtactcg tgctggtggc ggggattggc cagcggcagc gagagggcgc ccacccgggc
     3421 gcggtagacg agcggcagca ccgcctcgag cgccacgtcg tgggtgtggg gtccgtcgta
     3481 gttgccccag cagacgtgca ggcggatgcg gtcgggcggg atccccgcgg tggcctggtt
     3541 gatggcctcc acgtgcagct ccacgctccg ctggaaagcc tcgagggaat cgttctggaa
     3601 gaagcgcacc cgctccatgg ccaggtcggg gcagtccacc tggagcagca gcccgcgcgc
     3661 gtggatgagc tcgtactcct tgcgcatctc ccgggccagc gccatgacgt actgttcatg
     3721 cgagcggtag tgggcggaga gcaggatggt ggcgatcacc ccgggcgagg ccgcggtcat
     3781 gaagcgctcg gcgaagcccg gcgcccggcc gtcggtgtgc cggaggaagt cgtcgcactc
     3841 ccgggccgcc ggccccaggt ccgcgtattc cacctccgcg atggcctgcg gcgcgtccgc
     3901 gatgcgggcc gcgtcacgcc ggcggcgggc cagcatctcc gcgtagtcgg ggaagtcgat
     3961 caggtcgcgg gcgggcagcc ggcggctggt gcctccgaag ccgcgcatac gcccggccac
     4021 gtaggtcgag aagccggcgc ggggctgctc gccgttgttg ccgacgtcga tgccgacgct
     4081 caactgtctc atcaccacgt ccaggaccgc ggcatcggtt tcccgcgcga gggcggccgc
     4141 gtccaccggc tcgccccggt cgcggcggac gagcaggtcg cggagcgcgg gcggtcgcgg
     4201 caggctgccc acgtgcgtgg tgaggatgcg ccgatcgctg cgcttcaagc tagccggccc
     4261 tgggagtgat ctggatcaga ggagccggct cggtctcgat ctggatcgtg gtgtgatcga
     4321 tcccgaagcg ggagtggagg acgaggtgca gcgcttcgag gatccggtcc gcgggcgcgc
     4381 ccgcctcgac cacgacgtgg gcgctcatcg cttcgcggcc ggaggtgagc gtccagacgt
     4441 gcaggtcgtg gacccgccgc accgccgggg ccgagcgcag cgcttcctcg atctcggtca
     4501 cgtccagatg gggcggcgcg ccctcgagca ggacgttcac ggcctggcgc agcagggccc
     4561 acgtgcgcgg caggatgaag agcgcgatca gcgcgcccgc gatgacgtcc gcgagcgtcc
     4621 atccggtcaa gatgatgacc aggcccgcca cgatgacgag ggcggagctg accgcgtcgc
     4681 ccaggacctc gaggtacgcg ctccgcacgt tgaggctctc ggccgcgccg ccgtgcagca
     4741 gccacatgcc ggccaggttc acgaggaggc ccagcgtcgc caccaccagg atcgggccgc
     4801 ccggcacgtg cggcggctgc cagaggcgct cgtaggtggt gaccaggatg gcgacggcca
     4861 tcacgcagag gacgacgccg ttgacgaggg cggccaggat ctccacgcgg tagtagccgt
     4921 aggtcttggc cggcgtggcc gggcgctcgg cgaaccagac cgcgagcagg ctcaggagca
     4981 cgccgcccgc gtccaccagc atgtggacgg cgtcggccag gagcgcgagg ctgcccgtcc
     5041 agagcccgac cgccgcctcc accaccagga acgcggaggt cagcccgaag acggccagca
     5101 ggtggcgctt gtggcgggag ccgtgagtgt gccggccgtg cacgggcgtg atcagcgggc
     5161 cgggggcagc accagccgcg cttctccctg acggatgccc tggctcgcga agaacccggc
     5221 gtgggcttcg atcgcgtagc ggaaggaccc gagcgggccg tacagctcgg tggggctcgg
     5281 gtccatcttg aggatggcga agatgcggcc gtcctccttg acgaaggcga tgtccagcgc
     5341 cgcgggcacg ttctgcatgt ggaactgggt gacgatctct cgcccgaagt cgaagaggat
     5401 caggtgccgc ttgatctcct cgggggtcgc gcactggaag ccgccggcct ggcgctcgcc
     5461 ggtgtcggcg gccttcacgc ggagcgcgat gctgcgggcg ccggcctgca cgcccaccat
     5521 ccggatcggc atgcccgcga aggcttcgcg ccagcgcggg cactcgggat tcggggcggg
     5581 gcccttggcc gggccctggg ccatggccgc cgggaccgtg aggacgagga gcagcgcggc
     5641 caggatggcg acccgcgagg ggatcatgga tgggcctccc gtgcggtcgc gggccaccgg
     5701 cggcgcgcca gcgacgcggc ggcgaagatg agcagcgcgg ccaggaccac cgcgcccccc
     5761 gcggccaggc gcaggtagta ggccgcgatg agaccggagg tcatggagag cagcgcgatg
     5821 ccgatggcga tcgcggtggc ggtgtgaaag ctccggccca gcgcgaagcc ggtgagcgcg
     5881 gggatcacga tcatcgcgga gacgagcagc acccccacca tgcgcatcgc caccacggtg
     5941 gtcagcgcgg tcagcacggt cagcagcagg ttgagcgcgc cgaccggcac gccgctggtc
     6001 cgggccaggt cctcgtggag cgtgatggcg aggagctgct tgtagaagcc gccgactgcg
     6061 acggtgacga cggcgcccag ggccacgatg agccacacat caccgctggt gacggtgagg
     6121 atgctgccga agaggatcgc gaagagatcg gcgttgaagc cgcgggccag gctgatgagc
     6181 accaccgcca gcgcgaagcc gccggagagg aataccgcga gggccgcgtc gccctggagc
     6241 gcgccgcgcg cgcgcagccg ctcgatgccg acggcgccgg cgagggccac caccagcgcg
     6301 cccagcaccg gcgagatccc gagcacgagg cccagggcta ccccggccag cgccacgtgg
     6361 gccagcgtgt cggcgatcag cgagagccgg cgcgggatca ggaagacgcc gatggccggg
     6421 cagatgagcg cggtgaccac acccgccgcg aacgcccgat gcatgaagcc gtactggagg
     6481 aactcgggca tgggctcagt ggtcgtggct gaccacgcgc accgcggggc cgtagagcgc
     6541 ggtcagcgcc gcgtcgctca ggaaatcgcc cgggcggccg tggaagacga gccggcggtt
     6601 gagacaggcg agcttggtga cctccttggc caccaccccg atgtcgtggc ttaccaggat
     6661 cagggtcacg tcgcgctcgc gattgagatg gtggagcagg gcgtagaagc tcgtctgggc
     6721 ctcggggtcc acgccgccgg tgggctcgtc caggatcagg agctcgggct tggagaccag
     6781 cgcccgcgcg atcagcacgc gctgctgctg gcccggcgac agcgcgccga tccgcgcgcc
     6841 cgcgtgcgca tccatgccca cctggccgag cacctccgcg atgcgcttgc gctccgttcc
     6901 gccgatgcgt ccgaagaggc cgagcgaggg caggagcccg gtggccacca cctcgtggac
     6961 ggtcaccggc agcgcgggat cgagcgtggc cttctgcggc acgtagccga ggcgccccca
     7021 ctgccggaac gatgcgggcg ggtggccgaa gaggcggacc gtgccgtgct ggggcggcag
     7081 gaggcccaac gtgatgcgga gcagggtggt cttgccggag ccgttcgggc cgatgatgcc
     7141 gaggaagtcg ccgggctcga cggtcaggtt gatgcgctcg agcacgggga cggccccgta
     7201 ggcgaagtcc acgtcctcca gctcgacgaa ggagcgcatc agccgccgat ctcgagcgcg
     7261 tcgttcaccg cgcgcggccg cgccggctgc gtggtctcgg gatgccgcgc gtcccaccag
     7321 gcccccgcgg cggcgcagcc ggcgccgagc aggacgccgc ccagcacgtc ggtgggccag
     7381 tgggcgttca ggatgacgcg cgcgaagccg accgcgatga ccagcgcgaa cgccagcgcg
     7441 gtgaggatcc cgcgcgccgc gggggagacc cgctcccgcg tcatgatgta gagcaggatc
     7501 accgcgaacg tcgccgcggc ggtcacgtgc ccgctcggga agccccagtt gaccccgcgg
     7561 ggccgcggcc ggccgaccag gaacttgaag gcctgctcga cggcgccccc gatcggcatc
     7621 agggcgcacc agagccacca gtgccggcgg gccgcgcgcg accagacgaa gagcagcgcg
     7681 atggccggcc ctagcacgat ccacttgccg ccgtagtcca cccagcgggc gacggcgtgc
     7741 agcagggtgc cgcggccctt caggatctcg tcgtgcagcg gcacgtcgcc cggcagcagc
     7801 ccgaccgcgg cgaccacgag gctcatcccg aggaaggcgc ccagcaggac caggcagagc
     7861 gcccgccagc gcgcgacggt ggctgcgtcc atggcgatga ttgtactcga agctgggctc
     7921 gccggagcag ggtcggcctg gtaactcatg gaggttcgcg gtgggagtgg agggtgtggt
     7981 tgctgtgcag tcatactgga gcccaagggg gcggcctcta gagggcggcc cctccccctc
     8041 gggcgaaggc cgggcgcgtc ggctgacgcc cggctattcc aacttacatc gtccgcctaa
     8101 cgcctgccgt ggtgatgatg cccaccccag ccgggtccga ccggtatcgg cacgatgcag
     8161 gcttgagttc caacggtaac agccaccagc agcaacacga tcctcagata gcgcatagtg
     8221 cacctccttg gatcatcgga cgaactgtgg ggggcaggca ttcgtgaccc gacgcagtgg
     8281 acggcactgg gtcaggctga gttgctgact gccctgcgcg gccttggcgt cgaggagtcg
     8341 ggtcaggaac cacgagcccc tggccattga ccgagcttgc cgagtccgac catcaccaag
     8401 ttggcagcgc tcccacctgg acgcaggagc agcggcgaga tgacgaagag gacgccgacg
     8461 aggccgagcg tgccggtgta ggtgttcagc catcgcgtcg gcagaggcgg cggctcggtc
     8521 atcgtcacac ccgccgcagg agggcctcgg cgaa
//
LOCUS       DASUFA010000270         6377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1309160, whole genome shotgun sequence.
ACCESSION   DASUFA010000270 DASUFA010000000
VERSION     DASUFA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309160"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFX87_10055"
     CDS             <1..690
                     /locus_tag="VFX87_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX4995303.1"
                     /translation="AGYKTGEGMDPVLYAQFQPLEEALSALGVVVWPMVEFEADDALA
                     AAAAMAAADTRVEQVVLCTPDKDLAQCVRGDRVVQLDRRTRELRNEAAVQQKFGVLPA
                     SIPDWLALVGDSADGFPGLPGWGARSAATVLARYQHLERIPKLAMEWDVSVRGALRLA
                     TTLTEQREHALLFRVLATLRADAPIGTDVDALRWVGPHPGFAAWAERLGTPALHERAA
                     SLAATRFATTR"
     gene            687..1922
                     /locus_tag="VFX87_10060"
     CDS             687..1922
                     /locus_tag="VFX87_10060"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995304.1"
                     /translation="MSGRPARLAALRHRDFRLFWSGQLVSLIGTWMQSVGQAWLVLEL
                     TGSPFHLGLVTALQFTPILLLSPVGGALSDRFPKRRIILLTQATMMAQAFVLAGLVWS
                     GQVRYWHVAVLAATYGLGRAVDIPARQSYITDLVGRPDLPNAVALNSLVFNGARIVGP
                     AAAGLLIARFGVALAFFLNGLSFVAVLAALLATRTPGDPEPAGRLGIREGVWGALAYA
                     ARTPSTAFTLSLVVVVSVLVLNFNVVVPLVAKDVLNEGAHGFGLLMSSLGAGAVMAGV
                     GLTLLRRGRPSLWFLAASAAVLSAGTASLALVGHFGAAAVLLVALGCCQILFSTGCNT
                     TLQLDTPDALRGRVMGLYALANAGMTPFGSLLVGIIAERLGVRAACALGGGSGLVAVI
                     VLVLIARRTDLDWTHRRER"
     gene            complement(1927..2310)
                     /locus_tag="VFX87_10065"
     CDS             complement(1927..2310)
                     /locus_tag="VFX87_10065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011849779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4995305.1"
                     /translation="MKKEIVRVTLAHANPNLSPATRFGNLVFVAGQTGRHPVTGELGR
                     DIREQTRNTLERIKIVLAAAGTSLDNVLSAMTHLTRREDLAAYNEEYARYFPADKPAR
                     TTVTVASLNAPELLVEITVTACVPD"
     gene            complement(2319..3521)
                     /locus_tag="VFX87_10070"
     CDS             complement(2319..3521)
                     /locus_tag="VFX87_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4995306.1"
                     /translation="MRMPLEDVKVLDLSHALAGPFCSTMLADFGAEVIKLEPRGAGDI
                     ARAWGPPLPGGETSYFVSLHRNKKGIEVDLKHPEGKGLFFQLVDKCDVVLENYRVGAL
                     ARLGLGYEAARERNPGIIYCSVSGFGQDGPYRDRAALDLILQAESGMISTTGEPGGHG
                     VRCGVSIADMTAGMYAAFGIMLALRVKERTGRGQAIDVSMLEGQLSLLGSMIGAYFAD
                     GELPLPMGTAYKALLPYQTFRTKTRDLALAVGSEKLWKVFCPAIGRPELTDDPRYRTN
                     ADRARNRDTLIPKLQEAFLTRTYEEWEPLLLAQGIPMGAINTIPQVVEHPQVIARQAL
                     VEMAHPRAGKVRMVGAPVRLSETPGSVRTPAPLLGEHTDAVLRELLGLEAAEIDTLRA
                     GGVIGPKR"
     gene            complement(3580..4857)
                     /locus_tag="VFX87_10075"
     CDS             complement(3580..4857)
                     /locus_tag="VFX87_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4995307.1"
                     /translation="MSEPTGDDRAREAVEAVYRSDSRRVLATLIRLLGDFDLAEEALH
                     EAFAAAVEQWPREGTPANPRAWLVSTGRFKAIDAMRRRARFDASQAELAERLEAETAG
                     PRPGEAEDIVDDRLRLIFTCCHPALPPDAQVALTLREVCGLTTEEIARAFLTGPPTLA
                     QRIVRAKAKIRDARIPYEVPALAELPDRLDTVLHVVYLVFNEGYSASSGESLTRADLS
                     SEAIRLGRLLVELLPEPEAMGLLALMLLHESRRPARTSPTGELILLDDQDRSLWSRAQ
                     IAEGTALVERALASRRIGPYTLQAAIAAVHAQAPAAAATDWAQIVGLYDVLARAEPSP
                     VVELNRAAAVAMRDGPAAGLALIDAILARGDLADYPLAHSARAELCRRLGKTGDARAS
                     YERALGLTRQEPQRRFLERRLAELRDRGAAPAV"
     gene            complement(4876..5232)
                     /locus_tag="VFX87_10080"
     CDS             complement(4876..5232)
                     /locus_tag="VFX87_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4995308.1"
                     /translation="MKFMFLIYHDENTLDALPDGEMQGLVDAALGYDEELRQSGHYIV
                     SNALQRGRTARTIRVRGGGVSITDGPFAETKEQLGGFFLIEAKDIDEACAVASRFPPA
                     RIGTIEVRPVQELRHS"
     gene            complement(5229..5582)
                     /locus_tag="VFX87_10085"
     CDS             complement(5229..5582)
                     /locus_tag="VFX87_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4995309.1"
                     /translation="MKYVCLVYLVEKEMNALSKREADACTDESLSYDEALRRSGHLIA
                     AHALQPVETATTIRVRSGKLSTTDGPFAETKEQLGGFILIEARDLNDAIQVAAKIPMG
                     RRGSIEVRPVKELKP"
     gene            5847..>6377
                     /locus_tag="VFX87_10090"
     CDS             5847..>6377
                     /locus_tag="VFX87_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995310.1"
                     /translation="MNIFYGWVIVAAGIVMTCVGIGSMLSLGVFLQPMSEAMGWSRTG
                     ISLAALLNFLCMGVGSFVWGALSDRFGTRVVVLSGGILLGAGLVAASQAATLGQFQLL
                     FGVIVGVAAGSFYAPMTATTTRWFTEHRSLAVALVSAGLGLGSMTVAPLAAWIITTYD
                     WRVAMLAIGDLAWLLLI"
BASE COUNT          803 a         2222 c         2346 g         1006 t
ORIGIN      
        1 gcgggctaca agaccggcga gggcatggac ccggtgctct acgcccagtt ccagccgctg
       61 gaggaggcgc tgagcgcgct cggcgtggtc gtctggccca tggtcgagtt cgaggccgat
      121 gatgcgctgg ccgcggcggc agccatggcc gccgccgata cgcgcgtcga acaggtcgtc
      181 ctgtgcacgc cggacaagga tctggcgcag tgcgtccggg gcgaccgcgt cgttcagctc
      241 gatcggcgca cacgcgagct gcgcaacgag gcggcggtgc agcagaagtt cggcgtcctg
      301 cccgcgtcga ttcccgattg gctggcgctg gtgggggaca gcgcggacgg cttccccggc
      361 ctgcccggct ggggggccag gtccgcggcc acggtgctgg cccgctatca gcacctcgag
      421 cgcattccga agctggccat ggagtgggac gtgtccgtgc gcggcgcgct gcgcctggcc
      481 acgacgctga cggagcagcg ggagcacgcg ctgctgttcc gggtgctcgc cacgctgcgc
      541 gcggacgcgc cgatcggcac cgacgtggac gcgctgcgat gggtcgggcc gcacccgggc
      601 ttcgcggcct gggccgagcg gctcggcacg ccggcgctcc acgagcgcgc cgcgagcctc
      661 gcggccaccc gcttcgccac cacgcgttga gcggccgacc ggcgcgcctg gccgccctcc
      721 gccaccgcga cttccgtctc ttctggagcg ggcagctcgt ctcgctgatc ggcacctgga
      781 tgcagagcgt cggccaggcg tggctcgtgc tggagcttac cggctcgccc ttccatctcg
      841 gattggtgac cgcgctgcag ttcacgccga tcctgctgct ctcgccggtg ggcggcgcgc
      901 tgagcgaccg ctttcccaag cggcgcatca tcctgctgac ccaggccacg atgatggcgc
      961 aggcgttcgt gctggccggg ctcgtctgga gcggccaggt gcgttactgg cacgtggcgg
     1021 tgctggccgc gacctacggc ctggggcggg ctgtcgacat cccggcgcgc cagtcgtaca
     1081 tcacggatct cgtggggcgg cccgacctgc cgaatgcggt cgcgctcaac tcgctggtct
     1141 tcaacggggc gcgcatcgtc gggcccgccg ccgccggcct gctcatcgcc cgcttcggcg
     1201 tggccctggc cttcttcctc aacggcctga gcttcgtcgc ggtgctggcc gcgctgctcg
     1261 ccacccgcac gcccggagac ccggaaccgg ccgggcgcct cggcatccgc gaaggggtct
     1321 ggggcgcgct cgcctacgcg gcgcgcacgc cctccaccgc gttcacgctc tccctggtcg
     1381 tcgtggtcag cgtgctcgtg ctcaacttca acgtggtggt gccgctggtc gcgaaggacg
     1441 tgctgaacga gggcgcacac gggttcggcc tgctcatgtc gtcgctcggg gcgggggcgg
     1501 tgatggcggg agtcgggttg acgctgctgc gacgaggccg accatccctg tggttcctgg
     1561 ccgcctcggc ggcggtcctc agcgcgggca ccgcctcgct cgccctggtc gggcatttcg
     1621 gagccgcggc ggtcttgctc gtggcgctcg ggtgctgtca gatcctgttc tcgacgggct
     1681 gcaacaccac gctccagctc gacacaccgg acgcgctgcg cgggcgcgtg atgggcctct
     1741 acgcgctcgc caatgccggc atgaccccct tcgggtcgct cctggtgggc atcatcgccg
     1801 agcgcctggg ggtgcgcgcg gcctgcgcgc tgggcggcgg gagcgggctg gtggcggtga
     1861 tcgtcctcgt gctcatcgcg cgccgcacgg atctcgactg gacccaccgc cgcgagcggt
     1921 aggcggtcag tcgggaacgc acgcggtgac ggtgatctcc acgagcagct cgggcgcgtt
     1981 cagcgaggcg acggtgacgg tcgtccgcgc cggcttgtcg gcggggaagt acctcgcgta
     2041 ttcctcgttg taggccgcca ggtcctcccg ccgggtgaga tgggtcatcg cgctgagcac
     2101 gttgtcgagc gaggtgcccg ccgcggcgag gacgatcttg atgcgctcga gcgtgttgcg
     2161 ggtctgctcg cggatgtccc ggccgagctc gccggtgacc ggatgccgcc cggtctggcc
     2221 ggccacgaag acgaggttgc cgaaccgggt cgccggagag agattcggat tcgcgtgcgc
     2281 cagcgtgacc cggacgatct ctttcttcat ggcgcggctc agcgcttcgg gccgatgacg
     2341 ccgccggccc ggagggtgtc gatctcggcg gcctcgagtc ccaggagctc gcgcagcacc
     2401 gcgtcggtgt gctcgccgag cagcggggcc ggcgtgcgca ccgagcccgg cgtctccgag
     2461 agccgcaccg gcgcgccgac catccgcacc ttgccggcgc ggggatgggc catctccacc
     2521 agggcctggc gggcgatcac ctgggggtgc tccaccacct gggggatcgt gttgatggcg
     2581 cccatgggga tgccctgcgc gagcaggagc ggctcccatt cctcgtaggt ccgcgtgagg
     2641 aacgcctcct ggagcttcgg gatgagggtg tcgcggttcc gggcccggtc ggcgttggtg
     2701 cggtagcgcg gatcgtcggt cagctcggga cggccgatcg cggggcagaa caccttccag
     2761 agcttctcgc tgccgacggc cagggccagg tcccgcgtct tggtgcggaa ggtctggtag
     2821 ggcaagagcg ccttgtacgc ggtccccatc ggcagcggga gctcgccgtc ggcgaagtag
     2881 gcgccgatca tgctgccgag gagcgagagc tggccctcca gcatggagac gtcgatcgcc
     2941 tggccgcggc cggtccgctc cttcacgcgc agggcgagca tgatgccgaa ggccgcgtac
     3001 atccccgcgg tcatgtcggc gatggagacg ccgcagcgca ctccgtgccc gcccggctcc
     3061 ccggtggtgc tgatcatccc gctctcggcc tgcaggatca gatcgagcgc ggcgcggtcg
     3121 cggtacgggc cgtcctggcc gaagccggag accgagcagt agatgatgcc ggggttccgc
     3181 tcgcgcgcgg cctcgtagcc caggccgagc cgggcgaggg cgcccacccg gtagttctcg
     3241 agcacgacgt cgcacttgtc aaccagctga aagaagagac cctttccttc agggtgcttc
     3301 agatcgacct cgatgccttt cttgttgcga tgcaggctca cgaagtagga ggtctcgccg
     3361 ccgggcaggg gcggccccca ggcgcgcgcg atgtcgccgg cgccgcgcgg ctcgagcttg
     3421 atgacctcgg cgccgaagtc ggccagcatc gtggagcaga agggccccgc cagggcgtgg
     3481 ctcaggtcga ggaccttcac gtcttcgagc ggcatgcgca tcgagcggcc tccggaacgg
     3541 cgagagtgag gcgatcctgc cagatcatcc agggccaggt caaaccgcgg gggccgcgcc
     3601 tcggtcccgc agctcggcca ggcggcgctc gagaaagcgc cgctgcggct cctgccgggt
     3661 caggccgagc gcgcgctcgt aggaggcgcg ggcgtcaccc gtctttccga gccgccggca
     3721 cagctccgcg cgcgccgagt gcgccagggg gtaatcggcc aggtcgccgc gcgccaggat
     3781 cgcgtcgatg agggccaggc cggccgcggg cccgtcgcgc atcgccaccg cggcggcgcg
     3841 gttcagctcg accaccggcg acggctcggc ccgcgccagc acgtcgtaga gcccgacgat
     3901 ctgggcccag tcggtcgcgg cggcggcggg ggcctgcgcg tgcaccgccg cgatcgcggc
     3961 ctggagggtg tacgggccga tccggcgtga cgcgagcgcc cgctccacca gcgccgtgcc
     4021 ctccgcgatc tgcgcgcggc tccagaggga gcggtcctgg tcgtcgagca ggatcagctc
     4081 gccggtgggc gaggtgcgcg cggggcgccg cgattcgtgc agcagcatga gcgccagcag
     4141 gcccatggcc tcgggctcgg gcagcagctc gacgagcagc cgccccaggc ggatggcctc
     4201 gctcgagaga tcggcccgcg tcagcgattc gcccgaggag gccgagtacc cttcgttgaa
     4261 caccaggtag actacgtgga gaacggtgtc gaggcgatcc ggtagctcgg ccagggccgg
     4321 gacctcgtac gggatgcggg cgtcgcggat cttcgccttg gcgcgaacga tgcgctgggc
     4381 gagcgtcggg gggccggtga ggaaggcgcg cgcgatctcc tcggtcgtga ggccacacac
     4441 ctcgcggaga gtcagcgcga cctgggcgtc cggcggcagg gccgggtggc agcacgtgaa
     4501 gatcagccgg agccggtcgt ccacgatgtc ctcggcttcc cccggcctgg ggccggcggt
     4561 ctctgcctcg agtcgctcgg ccagctccgc ctgggaggcg tcgaaccggg cgcggcggcg
     4621 catggcgtcg atggccttga agcggccggt ggagacgagc caggcccgcg gattggccgg
     4681 cgtgccttcc cgaggccact gctcgaccgc ggccgcgaag gcctcgtgca gcgcctcctc
     4741 ggcgagatcg aagtccccga gcaggcggat gagggtggcg aggacgcggc gcgagtccga
     4801 acggtagacc gcctccaccg cctcgcgcgc gcggtcgtcg cccgtcggct cgctcatcgc
     4861 gcgtcgggcc cgcgactagg agtgccgcag ctcctgcacc ggccgcacct cgatggtccc
     4921 gatccgcgcc ggcgggaatc gcgaggccac cgcgcacgcc tcgtcgatgt ccttggcctc
     4981 gatcaggaag aagccgccga gctgctcctt cgtctcggcg aagggcccgt cggtgatgga
     5041 cacgccgccg ccgcgcaccc ggatcgtccg ggcggtccgc ccgcgctgca gcgcattcga
     5101 gacgatatag tggccgctct gccgcagctc ctcgtcgtag cccagggccg cgtccacgag
     5161 gccctgcatc tccccgtcgg gcaaggcgtc cagcgtgttc tcgtcgtgat agatcaggaa
     5221 catgaacttc atggcttgag ctccttgact ggtcgcacct cgatgctgcc ccggcgaccc
     5281 atggggatct tcgcggccac ctggatggcg tcgttcaggt cgcgcgcctc gatcaggatg
     5341 aacccgccga gctgctcctt cgtctcggcg aaggggccgt cggtcgtgga cagcttgccg
     5401 ctccggaccc ggatcgtggt ggccgtctcg accggctgga gggcatgggc ggcgatgagg
     5461 tgcccgctcc gccggagggc ctcgtcgtag gacaggctct cgtcggtgca ggcgtcggcc
     5521 tcccgcttgg acagggcgtt catctccttt tccacaagat agaccaggca gacgtacttc
     5581 atagcccctc ctcgagattg gatggctgcg gacgccagat ggtcgctcgg gacactcgga
     5641 aatcgacagc gatcgtccga tcttgcccgg catggcgccg ctgtcgagcc ctgtcgatcc
     5701 ccggccgacc cgttcgacca tggcgtgagg ggatgcagac caccgccgac gggccacgcc
     5761 cactggagag ccatgatgct gaaggacccc gaaggcaacc tgatccagct cctgcagccg
     5821 gctcgtggta ggctcctcgg tgcgcgatga acatcttcta cggctgggtc attgtggcgg
     5881 ccggcatcgt gatgacgtgc gtcgggatcg gctcgatgct ctcgctgggc gtgttcctgc
     5941 agcccatgtc cgaggcgatg ggatggtccc gcacgggcat ctcgctggcc gcgctgctga
     6001 acttcctctg catgggcgtc gggtcgttcg tctggggcgc gctctccgac cggttcggca
     6061 cccgcgtggt ggtgctctcc ggcggcatcc tgctgggcgc aggcctggtg gccgcgagcc
     6121 aggcggccac cctcggccag ttccagctcc tgttcggggt gatcgtgggc gtggcggccg
     6181 ggagcttcta cgcccccatg accgcgacga ccacccgctg gttcaccgag catcgcagcc
     6241 tggcggtcgc gctcgtctcg gcggggctcg gcctcggctc gatgacggtg gcgcccctcg
     6301 cggcctggat catcacgacc tacgattggc gcgtcgcgat gctggcgatc ggcgacctcg
     6361 cgtggctcct gctcatt
//
LOCUS       DASUFA010000271         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1309212, whole genome shotgun sequence.
ACCESSION   DASUFA010000271 DASUFA010000000
VERSION     DASUFA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309212"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFX87_10095"
     CDS             <1..321
                     /locus_tag="VFX87_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4995311.1"
                     /translation="LRAALQARTALEWEAHFGERVPCAAARPGEDMFDHPQVAAEGIL
                     ASFEHPGVGHYRGMAHPVRFGDGPPPAPFAAPQLGQHSREILAGFGYPAEEIERLCGA
                     GVVI"
     gene            324..1220
                     /locus_tag="VFX87_10100"
     CDS             324..1220
                     /locus_tag="VFX87_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX4995312.1"
                     /translation="MADLLMRSKLFVPASRPELFAKALAGDADALSFDLEDAVQESRK
                     GEARRTLEVFLRQASERPPGKILIVRVNGLTTPHFEADLRAVAWAGVDMINLPKPESA
                     EDVRAAAAALARVEAERRIGRPVGILANIESPRGLRLALQIATAHPRVVGLQLGLGDL
                     FEPFGIDRADARAVHATQLAVRLAAAEAGIWACDTVYGTVADPEGFTREAETARRLGF
                     IGKSCIHPTQVPLANAVFRPTDSEITAAIRIVEAARGAERNGVGAFLVDGRMIDIPFV
                     KRAESVLGAARRLGLISPPAGG"
     gene            1404..2672
                     /locus_tag="VFX87_10105"
     CDS             1404..2672
                     /locus_tag="VFX87_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995313.1"
                     /translation="MRFGIVVMALTLGVAHAGPALAQTPSLSDAECQSLRQRLGEHAR
                     LSEGVRKAVATQAAAAPAVATPAPSATPAPTGRAEVIRARLEQIPKDRQTLEDQRLAA
                     MVKFELARAGQIQGQIQALDAEKATLEREQAALPAGAPASTVTAPQALVSDVARIRCQ
                     DMPAAVDNAVKIRRRELGAREDQAGAIPLLTLKGQTADQLGQELAGQFSPGAAAGTQV
                     GLLDSNGDGKLDGFVDVPAPGVFRLFRQRADGTVGVEVFPASASGAAPAYGEMTRRLD
                     ETLLRQASQGLPELLATRAAGPLRGVTQTTEFGPAHAQYQAGNFADAARLGTAAARSM
                     EFQNVRGQSVRMLEIISPVAGGVSVRRAVVLGQPNDQELWEETTTIVRPTSYWRTDVE
                     VARSRETRTTAGALVGTPSVSAPSRFTLER"
     gene            2692..2901
                     /locus_tag="VFX87_10110"
     CDS             2692..2901
                     /locus_tag="VFX87_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX4995314.1"
                     /translation="MLKRTITTFIGLAMLSAAVTACGTLTGAAVGAAGGAAVGAGTGY
                     GAGKGALIGTGVGAAAGAIYDFTKK"
     gene            3017..3196
                     /locus_tag="VFX87_10115"
     CDS             3017..3196
                     /locus_tag="VFX87_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995315.1"
                     /translation="MTPQKVEGQVIKVDAAQNKLTVRQTDGTVHEFQASAEAIQGFKV
                     GDRIEANLREAPKCR"
     gene            complement(3209..3544)
                     /locus_tag="VFX87_10120"
     CDS             complement(3209..3544)
                     /locus_tag="VFX87_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4995316.1"
                     /translation="MKRAKSPLSREALELIAQRFRALADPTRLALLQAMFEGEQTVQD
                     LCAITGASQANTSKHLALLLEQGLVARRRDGLFTRYRIADPTLERLCRLVCGSLAERS
                     ETVRNHLAS"
     gene            complement(3552..>4494)
                     /locus_tag="VFX87_10125"
     CDS             complement(3552..>4494)
                     /locus_tag="VFX87_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4995317.1"
                     /translation="PGHTPDSIAVLVEGHLFTGDALFAGGAGRTDFMGGSSSELFDTL
                     RGFASLPDATVVHPGHDYVGRAVTSIGEEKAGNPLLREPDRAAFVARLSAKSAPPANM
                     AAILRHNLGEADAATITAEELQALRQQGSGPLLLDVRSPIEFESERIDGALNVPLDAL
                     DARRDEIPDGADLVVVCRTGVRATIAAESLARTGCGARVLDGGMQAWRRARLPMREGR
                     KRLPVDRQVQMIAGVMVLTGVGLGLAVSPWFLALAAFFGAGLTFAGATGTCGLALLLM
                     KLPWNAPRLLPGDTGGAVCAAGGGAAPGCAAPTPPRV"
BASE COUNT          667 a         1568 c         1678 g          581 t
ORIGIN      
        1 ctgcgcgccg cgctgcaggc gcgcaccgcg ctggagtggg aggcgcactt cggcgagcgc
       61 gtgccgtgcg cggcggcgcg cccgggcgag gacatgttcg accatccgca ggtggccgcc
      121 gagggcatcc tggcgagctt cgagcatccg ggcgtgggcc actaccgcgg catggcccat
      181 ccggtgcgct tcggcgacgg cccgccgccc gcccccttcg ccgcgccgca gctcgggcag
      241 cactcgcggg agatcctcgc gggcttcggc tacccggccg aggagatcga gcggctctgc
      301 ggcgcgggcg tggtgatctg atcgtggccg atctcctcat gcgcagcaag ctcttcgtgc
      361 ccgcgtcgcg gcccgagctc ttcgcgaagg cgctggccgg cgacgcggat gcgctctcgt
      421 tcgacctcga ggacgcggtg caggagagcc gcaagggcga ggcgcgccgg acgctcgagg
      481 tgtttctccg gcaggcctcc gagcggccgc ccggcaagat cctcatcgtg agagtgaacg
      541 ggctgacgac gccccacttc gaggccgatc tacgggcggt ggcgtgggcg ggagtggaca
      601 tgatcaacct gcccaagccc gagtccgcgg aggacgtccg ggcggcggcg gccgcgctcg
      661 cccgcgtgga ggcggagcgc cgcatcgggc gccccgtcgg catcctcgcg aacatcgaat
      721 cgccgcgagg gctgcggctg gccctgcaga tcgcgacggc acacccgcgt gtcgtcgggc
      781 tccagctcgg cctgggcgat ctcttcgagc ccttcggcat cgatcgggcg gacgcccgtg
      841 cggtccacgc cacgcagctc gccgtgcgcc tggccgcggc ggaggcgggc atctgggcgt
      901 gcgacacggt ctacgggacg gtggcggatc ccgagggatt cacgcgcgag gcggagaccg
      961 cgcggcgcct cggcttcatc ggcaagtcct gcatccatcc cacccaggtc ccgctcgcga
     1021 acgcggtgtt ccgaccgacg gacagcgaga tcacggccgc cattcggatc gtcgaggcgg
     1081 cacgcggcgc cgagcgcaac ggggtcggag ccttcctggt ggacggccgg atgatcgaca
     1141 tcccgttcgt caagcgcgcc gagtcggttc tcggcgccgc ccgccggctc gggctgatct
     1201 cccccccggc ggggggatga gcggcagagg tccagagatt cgcatcgggg cgtgaagacc
     1261 ccacccgggg gaggtctgcc tcaccgggct cgagtcacat tcgcaggaac ctcgcgtttc
     1321 ggctaagttc tcgacctggc accacggatg cactctgccg ggtagtcggt gtgccctggt
     1381 acgccgcgag aggaggacgg tctatgcgat tcggcatcgt ggtcatggct ctcacgctcg
     1441 gggtggccca cgccggtccg gccctggctc aaacgccgag cctctcggat gcggaatgcc
     1501 agagcctgcg ccagcggctc ggcgagcatg cgcggctgtc cgagggtgta cggaaggcag
     1561 tggcgaccca ggcggcggcc gcgccggccg tcgccacacc ggccccgtcc gccacgcccg
     1621 caccgaccgg ccgggctgag gtgattcggg cccgcctcga gcagatcccc aaggatcggc
     1681 agacgctcga ggaccagcgg ctggccgcga tggtgaagtt cgagctggcg cgcgccggtc
     1741 agatccaggg acagatccag gccctcgacg cggaaaaggc cacgctggag cgcgagcagg
     1801 cggcgctccc ggcaggcgcc ccggcgtcca cggtgaccgc accacaggcg ctggtctccg
     1861 acgtggcccg catccgctgt caggacatgc cggcggcggt cgacaacgcc gtgaagattc
     1921 gacgccgtga gctcggcgcc cgggaggacc aggcgggcgc gatcccgctc ctcacgctga
     1981 aaggccagac cgcggatcag ctcggccagg agctggccgg gcagttctcg cccggcgccg
     2041 ccgccgggac gcaggtcggg ttgctcgact ccaacggcga cggcaagctc gacggcttcg
     2101 tcgacgtgcc cgcgccgggc gtcttcagac tcttccgcca gcgggccgac ggcacggtcg
     2161 gggtcgaggt gttcccggcc tccgcgagcg gggccgcccc ggcctatggc gagatgaccc
     2221 gtcggctgga cgagacgttg ctgcggcagg ccagtcaggg cctcccggag ctgctggcga
     2281 cccgggcggc cggcccgctc cgcggggtga cgcagaccac ggagttcggc ccggcgcacg
     2341 cgcagtacca ggccggcaac ttcgccgacg cggcacgtct cggcacggcc gcggcccgca
     2401 gcatggagtt ccagaacgtg cgcggccaga gtgtccggat gctcgagatc atcagcccgg
     2461 tcgccggggg agtgagcgtg cgccgggcgg tggtgctcgg acagccaaat gaccaggagc
     2521 tgtgggagga gacgacgacg atcgtccggc cgacttcgta ttggcggacg gacgtcgagg
     2581 tggcccggag ccgggagacg cgcacgacgg ccggcgcgct ggtgggaaca ccgtccgtct
     2641 cggcacccag caggttcacc ctagagcgct agacggagaa ggagagacat catgctcaag
     2701 cggacgatca cgacattcat cggcctggcc atgctctcgg cggcggtgac ggcctgcggg
     2761 accctgaccg gcgccgcggt gggcgcggcg gggggcgcgg cggtcggggc cggtaccggg
     2821 tacggcgccg gcaagggcgc cctcatcggc accggggtgg gcgccgcggc cggggcgatc
     2881 tacgacttca cgaagaaata aggagacgac catgctcaga tgggcggcag tcgcggcggg
     2941 acttgcaatg accctcacgc tctggtcggg gtttgcattc ggacaggcca aggcatgtga
     3001 ccagcaggga cgggtgatga ccccgcagaa ggtcgagggg caggtcatca aggtggacgc
     3061 ggcgcagaac aagctgactg tccggcagac cgacggcacc gtgcacgagt tccaggcctc
     3121 ggcggaagcg atccagggct tcaaggtggg cgaccggatc gaggccaacc tccgggaagc
     3181 gccgaagtgc cgctaggcgc tataccgttc aggacgcgag gtggttccgg acggtctcgc
     3241 tccgctcggc aagtgagccg cagaccagcc gacacagccg ctcgagagtg gggtccgcga
     3301 tccggtagcg ggtaaagagg ccgtcacgcc gccgggcgac gaggccctgc tcgaggagca
     3361 gcgccaggtg cttgctcgtg ttggcctgcg atgccccggt gatcgcgcag agatcctgca
     3421 cggtctgctc cccttcgaac atcgcctgca gcagggcgag ccgggtcgga tcggcgagcg
     3481 cccggaaccg ctgcgcgatc agctcgagcg cctcccgtga gagcggggac ttggcccgct
     3541 tcatggccac gtcagacccg gggcggcgtg ggcgcagcac agccgggcgc cgctccgccc
     3601 ccggccgcgc acacggcccc gccggtatcg ccgggcagga gacgcggcgc gttccagggg
     3661 agcttcatga gcaggagcgc aagcccgcac gtaccggtcg cgccggcgaa ggtcagcccc
     3721 gcgccgaaga aggcggcgag tgccaggaac cacgggctca ccgcgagccc caggccgacg
     3781 ccggtgagca ccatgacgcc ggcgatcatc tggacctgcc ggtcgacggg caggcgcttg
     3841 cgcccctcgc gcatgggcag acgagcacgg cgccatgcct gcattccgcc atcgagaacc
     3901 cgtgccccgc acccggtccg cgcgagcgac tctgccgcga tggtggcccg gactcccgtg
     3961 cggcacacga cgacgagatc cgctccatcg gggatctcgt cccggcgggc atcgagcgcg
     4021 tccaggggca cgttgagcgc gccatcgatg cgctcgctct cgaactcgat cgggcttctc
     4081 acgtcgagga gcagcggccc ggagccctgc tggcgaagcg cctggagctc ctcggccgtg
     4141 atcgtcgccg cgtcggcctc cccgagattg tgccgcagga tggccgccat gttggcggga
     4201 ggcgcggact tcgccgagag ccgggcgacg aaggcggccc ggtccggctc gcggagcagc
     4261 ggattgcccg ccttctcctc gccgatcgag gtcacggccc ggccgacata gtcgtgaccg
     4321 ggatgcacca ccgtggcgtc gggcaggctc gcgaagcccc ggagcgtgtc gaagagctcg
     4381 gaggagctgc cccccatgaa gtcggtccgg cccgcgccac cggcgaacag cgcgtccccc
     4441 gtgaagagat ggccctccac gagcaccgcg atggagtccg gcgtgtggcc cggg
//
LOCUS       DASUFA010000272         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_392424, whole genome shotgun sequence.
ACCESSION   DASUFA010000272 DASUFA010000000
VERSION     DASUFA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392424"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VFX87_10130"
     CDS             complement(<1..1125)
                     /locus_tag="VFX87_10130"
                     /inference="COORDINATES: protein motif:HMM:NF005687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4995318.1"
                     /translation="MPGGRAIATPLWRLDAVELAEMIRRRAVSCHEVVTAALARLDAV
                     NPRINAVVRPLHETALAEAAAADQALARGGAVGPLHGVPITTKANVDQAGCPTDNGVV
                     AFKDLVATRDNPVVANLRRAGAIVIGRTNTPAFSMRWFTDNALHGPTRNPWDPARTPG
                     GSSGGAAAATATGIGAIGHGNDIAGSVRYPAYCCGLVGLRVSYGRIPSWNFTATTLRP
                     ISSQLMAVQGPLTRRVRDARAAFAAMAVPDLNDPRCADVPLEGPPVKRPMRVALVADP
                     AGRGGVAPAVASAVRQAGRWLEDAGCVVEEVEPPELGAVADLWAAIAMEDVIAALEPA
                     AAHYGDAGIKQALGLWRAVHPPRDVRHVLDGLAQRDRLLRL"
     gene            complement(1104..>2088)
                     /locus_tag="VFX87_10135"
     CDS             complement(1104..>2088)
                     /locus_tag="VFX87_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4995319.1"
                     /translation="HEYPQQNRYKTISRYYGYHGTTLATLAAGGMGDRKMKFEPLSGN
                     EFVHVAPPYCYRCPFGLEPTSCDLACVKNMETTILGEGPQTVAEVIIEPVMSAVGVAV
                     PPDGYLPAVADLCKQHGILLHVDEVINGFGRTGRLFAHEHAGVRPDIVTVAKGISSAY
                     LPMAATVVRNSVFKSFIGEASENRQVNQVNTYGGHPVAAAVALRNIEIMLEEKLAQRS
                     AENGAYLLDGLKTLMRHPWVGDVRGKGLYAGVELVIDRKSKAIMPAPQIKKLVDRCQE
                     RGVIVGRSGGGAHLGNTLVLAPPLVLTRPEIDRIVSVLDQTIPEVCPEAGR"
BASE COUNT          271 a          730 c          789 g          298 t
ORIGIN      
        1 gaggcgcagc agccggtcgc gctgggccag gccgtcgagc acgtggcgga cgtcgcgggg
       61 cgggtgcacc gcccgccaga ggccgagcgc ctgcttgatg ccggcgtcgc cgtagtgcgc
      121 cgcggcgggc tccagcgccg cgatcacgtc ctccatcgcg atggcggccc agaggtcggc
      181 caccgcgccg agctcgggcg gctccacctc ctcgaccacg cagcccgcgt cctcgagcca
      241 gcgcccggcc tggcgcacgg cggacgccac cgcgggggcc acgccgccgc ggccggccgg
      301 atccgccacc agcgccacgc gcatgggccg cttcacgggc gggccttcga gcggcacgtc
      361 ggcgcagcgc ggatcgttga gatcgggtac cgccatcgcg gcgaaggcgg cgcgcgcgtc
      421 gcgcacccgc cgggtcagcg ggccctgcac ggccatgagc tgcgacgaga tcggccgcag
      481 cgtggtggcg gtgaagttcc acgaggggat gcggccgtag ctgacccgga gcccgaccag
      541 cccgcagcag taggccgggt agcgcacgga gccggcgatg tcgttgccgt ggccgatcgc
      601 gccgatgccg gttgcggtgg ccgcggcggc gcccccgctc gagccgcccg gcgtgcgcgc
      661 gggatcccac gggttgcggg tcgggccgtg gagcgcgttg tccgtgaacc agcgcatcga
      721 gaaggcgggc gtgttggtgc ggccgatcac gatggcgccc gcccggcgca ggttggccac
      781 caccgggttg tcgcgggtgg cgacgagatc cttgaacgcg accacgccgt tgtcggtggg
      841 gcaaccggcc tggtccacgt tggccttggt ggtgatgggt acgccgtgga gcgggccgac
      901 cgccccgccc cgggccaggg cctggtcggc ggctgccgcc tcggcaagcg cggtctcatg
      961 gagcgggcgc accaccgcat tgatccgcgg gttgaccgcg tcgagccgcg cgagcgccgc
     1021 ggtcacgact tcatggcagg agacggcccg ccggcggatc atctccgcca gctccaccgc
     1081 atcgagccgc cagagtgggg tggctatcgc ccggcctccg ggcagacctc gggaatggtc
     1141 tggtccagca ccgagacgat gcggtcgatc tcggggcggg tgaggacgag cggcggggcc
     1201 agcacgaggg tgttgccgag gtgcgcgccg ccgcccgagc ggcccacgat caccccgcgc
     1261 tcctggcagc ggtccaccag cttcttgatc tggggcgcgg gcatgatcgc cttggacttg
     1321 cggtcgatga ccagctcgac gcccgcgtag aggcccttgc cccgcacgtc gccgacccac
     1381 gggtggcgca tcagggtctt gagcccgtcg aggagatagg ccccgttctc ggccgaccgc
     1441 tgggccagct tctcctccag catgatctcg atgttgcgca gggccaccgc cgcggccacc
     1501 ggatgaccgc cgtaggtgtt gacctggttg acctggcggt tctccgacgc ctcgccgatg
     1561 aacgacttga agacggagtt gcgcaccacg gtggcggcca tcgggaggta ggcgctcgag
     1621 atgcccttgg ccaccgtgac gatgtcgggt ctgacgcccg cgtgctcgtg ggcgaagagc
     1681 cgcccggtgc ggccgaagcc gttgatgacc tcgtccacgt gcaggaggat gccgtgctgc
     1741 ttgcagaggt cggccaccgc gggcaggtag ccgtcggggg ggaccgccac cccgaccgcg
     1801 ctcatgaccg gttcgatgat cacctccgcc accgtctggg gcccctcacc caggatcgtc
     1861 gtctccatgt tcttcacgca ggccaggtcg cagctggtgg gctcgagccc gaacgggcag
     1921 cggtagcagt agggcggggc cacgtgcacg aactcgttgc cggagagcgg ctcgaacttc
     1981 atcttgcgat cgcccatgcc gcccgcggcg agggtggcga gcgtggtgcc gtggtagccg
     2041 tagtaccggc tgatcgtctt gtagcggttc tgctgcgggt actcgtgg
//
LOCUS       DASUFA010000273        20864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1310939, whole genome shotgun sequence.
ACCESSION   DASUFA010000273 DASUFA010000000
VERSION     DASUFA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 20864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20864
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310939"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VFX87_10140"
     CDS             complement(<1..1433)
                     /locus_tag="VFX87_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX4995320.1"
                     /translation="MSDGWGGRSRGLRRWTLLAGALLAIAVLVGGGLAIYAARALSRF
                     ERVEARRSTVLYAAPPVLRSGVNVGALDLAGILGRQGYRETRNAGAPGQFSRSESAWE
                     INVAGAARVTLTVSGGRITQLRQGGTEVQSVALPPELLASAGADMGENIRPVRLPDVP
                     RILRDAVLVTEDVRFYEHGGVDPRGIVRALWTNVRKGRVAEGGSTITQQLVKSRLLNP
                     ERTLARKANEALLSTALEWRYGKDQILEAYLNEIYLGQSAGSAVRGVGAASRAYFGKE
                     VHQLTLPEAALLAGMIRGPNNYSPVGNPERARERRDVVLTRMRDLGKISEADYRRARK
                     EPVRARTIPASGLMAPYFVDYVRAELERSTDIDLADLHGVRVYTTLDPVLQRLAEAAV
                     VKGMDRLETLRPRLRRKKPEERLQVAMIVLDPATGQVRALVGGRDYRASQFNRAVLAR
                     RQPGSAFKPFVFLAALTPRKDASLFTPA"
     gene            complement(1453..1848)
                     /locus_tag="VFX87_10145"
     CDS             complement(1453..1848)
                     /locus_tag="VFX87_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995321.1"
                     /translation="MSARVSSSGLARLLAGAALVAGLAAMGAGVAPAGERRAGSVLAV
                     DAEARTLTLDEFGANADRRALRVQVPREAVVLLSQRNQAGRDAKDSFRDSTIALADVR
                     VGDFVVIEMSGDPEVARLVMITLRRGAGS"
     gene            complement(1845..2471)
                     /locus_tag="VFX87_10150"
     CDS             complement(1845..2471)
                     /locus_tag="VFX87_10150"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995322.1"
                     /translation="MRLRLVPYLIPVVLVGLTVGAPASARAGAPTDQLKASVDQIVKT
                     LEDPALKPEARARERRAAIRKEAAGVFDFSETAKRALGRHWQGLAEKDREEFTALFTD
                     LIERAYISKIERYSGERIAYAGEAVDGGLATVRTRFVTKQGTEVPVDYRMQQRGDRWL
                     VYDVSVEGVSLINNYRTQFDKIIQTSSYAELVRKMKAAEFAAPASPRS"
     gene            complement(2475..2831)
                     /locus_tag="VFX87_10155"
     CDS             complement(2475..2831)
                     /locus_tag="VFX87_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995323.1"
                     /translation="MEMIVVGTMLLVVVLLGVAVKLYDLKRRREDEAIAAQARISDAL
                     LMDATLAGLPLTPTAHLPLWKGSPLVVEITGAVPRPELREAAVDVARREIGRNRPDFR
                     IEDRIAVDPAALGRAA"
     gene            complement(3018..3629)
                     /locus_tag="VFX87_10160"
     CDS             complement(3018..3629)
                     /locus_tag="VFX87_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX4995324.1"
                     /translation="MRSIGRMFNGPPRWRVAILESDSASRAGLRSAVEDAGGVVALEA
                     PLRLDAVAVMRETRPDVVIMAPSLSEAREPALMPRITAEVHCPAVLFTRDTDQSVVKH
                     ANRAGVMGFMLKPLRAAEVGPTLDLALARFREIRRLQQARAERPLVDQAKGKLMSDRQ
                     LSEAEAFRWLRRHAMDSRQRLGDVAREVLAGEAALQALPEASR"
     gene            3834..4637
                     /locus_tag="VFX87_10165"
     CDS             3834..4637
                     /locus_tag="VFX87_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995325.1"
                     /translation="MSLRLKREPEDEIWDERLPEPSDLLSSEEISPEDGVEVGEEYDR
                     DDENRGLRLLSAVPGPLIAAVTFVPTFLAIFFGLGYLLGGAAPTGPTASDPVPSATAL
                     ASDPGTQGTPPLPEALRDPLTGPRMVDSLSPRPSDPREPLPGSSAADTPAPAIESTGP
                     TLLPPTQPPAAVSSAPEPRVGRPAEPPRPAQAAVLNAPKEPTPAVEARKSGREWTPAA
                     AFADREAARRLAGSIQQQGYPVEIRQEQSSSRPWVVWIGAQPRGGERRR"
     gene            complement(4648..6018)
                     /locus_tag="VFX87_10170"
     CDS             complement(4648..6018)
                     /locus_tag="VFX87_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4995326.1"
                     /translation="MKHRILIADDDESGRGGLAALLAMWGYEVQEAVDGKDALERAPS
                     FRPHVVVADMVMPVIDGIGLLKPLAATVPEAAVILLTAHGSIETAVSAMREGAYDYMT
                     KPVDPRRLRVLVEKAVEKVQVNREVTVLRRQLKETRGLGPLLGVSPAMQEVYRLIEMA
                     APSPAPVLILGETGTGKELVARAIHELSQRAKGPFVAVNCSAIPETLLESELFGHEKG
                     AFTGAIARRPGYFELADGGTIFLDEIAEMSSSLQAKYLRVLQDSQVRRIGGHDEVAVD
                     VRVVCATNRDVVKAVQDGTFREDLYYRVNVLTVPLPPLRRRPEDIPLLAEAFMAEFND
                     KYERQVRGIDEAALGLLREHAWPGNVRELRNAIERAVVSCEDGMITANCLPIAPLSGQ
                     ATERANVVVLPVGTTLDDGERQLILRTLQSVNNNKTRAAEILGTTPKTLHNKARRWRE
                     LQAEQR"
     gene            complement(6240..6689)
                     /locus_tag="VFX87_10175"
     CDS             complement(6240..6689)
                     /locus_tag="VFX87_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEX4995327.1"
                     /translation="MKIQQLDEVEGRRVFVMVCDRGDEAVEALTVAAKRFDLTGAALT
                     GIGAFSGVTLGFLDRARPDYARRVIRERVEVVSLVGNVALDAGEPRVHVHVVVARYDG
                     SALGGHLLGGTVDATLEVTIVESPKTLRRKTDPATGLALVDVDWSRS"
     gene            7012..7716
                     /locus_tag="VFX87_10180"
     CDS             7012..7716
                     /locus_tag="VFX87_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995328.1"
                     /translation="MTDMWGDLVWAALADLKTRLVLVMPGILAMLTLAVVGLAGAWIA
                     ARVTRRVAQAVAFDRRAENWGIVAQLSRAGVRRPPSQVLGLVVFWSILVLFLSMAVDA
                     LAIPGTGRLTEFMFSWVPRVMGAALILLVGWLVANFLGEGALIAAVNARVPEARLLAR
                     AIRWGVLLFASATAITHLGIGKEMVLVAFGITFGGLIFALALAFGLGGRALARQILER
                     HLRNREPHPRETLTHL"
     gene            7720..10746
                     /gene="treY"
                     /locus_tag="VFX87_10185"
     CDS             7720..10746
                     /gene="treY"
                     /locus_tag="VFX87_10185"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX4995329.1"
                     /translation="MIDVDVLFQQAAAALRRERHATYRLQLGSTLGFDAVAALGPYLE
                     ALGVSDAYLSPCFRCGPGSSHGYDVTDHNSFNPEIGGAAAFDRLVAALTARGLGLILD
                     VVPNHMGIAGDANPWWLDVLENGQASPRAGFFDIDWTPIKPELRDRVLLPVLPEQYGR
                     VLESQQLQLEFADGAFHLRYAGARLPIAPDTYAQILTDRLDVLAGRLGPEDAGLQELR
                     SILTSLEHLPGRTETDPARLEERLREKEIVKRRLAVLTKESADIREHVEETVRAVNGA
                     PGDPASFDALDRLLSAQTYRLADWRVAGDEVNYRRFFDVSHLAAIRMEERFVFDAAHE
                     LVFRLIGEGKVTALRVDHPDGLYAPGEYFRRLQEGALVATARRLLPELAPAEAEALGA
                     LYRARAAERPAAPDARPLWIAAEKILSPGEPLPDWWAVAGTTGYDFLASVNGLFVDRG
                     TSRQMTALYTRLAGAGGLLADHMYTAKRLIMQVSMASEINQLGHHLDSMSERNRLSRD
                     FTLYSLVRVIRELVAAFSVYRTYVGDDGRDPSVRDRAYIEEAVAEAKRRNPTVNVSIF
                     DFVRDVLLVRSPSRPDPDEQAARRHFAMRFQQTTGPVTAKGVEDTAFYVYNRLVSLNE
                     VGGDPARYGESPTVFHDKNAARLARWPESLVATATHDTKRGEDVRARINVLSEVPAAW
                     ATEVRRWRALARRFKVDVDGRAAPDANDEYLFYQTLVGAWPARDEDEPLETLTERLGA
                     YMEKATKEAKRHTSWVNPSAGYDAATRGFVAGLLAPGGVFLSAFRPFQRLVAAHGAVN
                     SLAQTLLKIVAPGIPDLYQGCELWDLSLVDPDNRRPVDFAHRRALLDTLRARIPADDA
                     GAPDLSALCAELLDHWQDGRVKLFLTHRALTLRRQRARLFGAGEYRGLAGGGRHADHV
                     VALARLDGADAVIAAVPRLTARLAGLTGRVALGEAAWEQTWVALGDERLAGVYRDRFT
                     GLTVASERRDGIAALSAPALFRHFPVALLEREGRKP"
     gene            10743..12587
                     /gene="treZ"
                     /locus_tag="VFX87_10190"
     CDS             10743..12587
                     /gene="treZ"
                     /locus_tag="VFX87_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX4995330.1"
                     /translation="MSPGPAARRAHRMPFGAEVREDGTTRFRLWAPGARRVDLWLEEP
                     GRPVAMPRDAGGWAEYVTREAPPGTRYRYRIDGEFLVPDPAARFQPGDVHGASEVVDP
                     LAHAWTDTAWNGLPRERLVFYELHVGAYTPGGTYAGVAERLDHLVSLGVTAVELMPLA
                     DFPGRWGWGYDGVLLYAPEATYGPPEDLKALVDAAHARGLAVFLDVVYNHFGPEGNYL
                     HRYAPGFFNPRHQTPWGDGVNYDGPGSEVVRAFMIHNALYWIEEYHLDGLRLDAVNAI
                     VDDSPRHLLVELARAVADGPGRERRVHLVLENGANEARYLRREDGGRPLYQAQWNDDV
                     HHALHVLLTGESGGYYGDYQPPLPHLGRCLTEGFAFQGERSVYRDGARGEPCADLPPT
                     AFVGFLQNHDQIGNRALGERLAALAPVDAVRAATALLLLSPGLPLLFMGEEWAAPEPF
                     LFFSDLGPDLGPLVSEGRRRELARFPEFADPAMRERIPDPQAESTFRRSVLDWTHASA
                     AVHKDSLAFHRTLLALRARAVAPLLADEAVPVGWWKALGDTALEVGWTFPSGTLRLVA
                     NLGAAPVPHEGPGADWGRRLHALALPSPAWSTLPPWSVAFYLAGARPR"
     gene            complement(12608..13783)
                     /gene="glgA"
                     /locus_tag="VFX87_10195"
     CDS             complement(12608..13783)
                     /gene="glgA"
                     /locus_tag="VFX87_10195"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX4995331.1"
                     /translation="MRILLLSREYPPHVYGGAGVVVDHLSRALARCASVEVRCFGEQE
                     QARPPLTVRGYAPWPRLAGGPEAPYAPALEALSVGLAMARDPVAADVVHTHTWYVGLG
                     GMLVQSIHDVPLVVTLHSMEPLRPWKADQLGTGYAVSSWAERLAVERADRVIAVSAQM
                     RADIMTHFQVDPARVVVIHNGVDAEAFARTERRDALARHGVRAPYVLFVGRISEQKGI
                     FQLLEAARALPAGVQLVLCASSPDTPELLARLEAAVAGRPEIRWINAMLPVPEVVQLY
                     SHAAVFVCPSIYEPFGLINLEAMACGTPVVASRVGGIPEVVVDGETGRLLEPGDAAAL
                     GQALRETLADPERARRMGEAGRRRVEAHFSWDRIADRTMTVYREAIDSKSARGGGPH"
     gene            13813..14475
                     /locus_tag="VFX87_10200"
     CDS             13813..14475
                     /locus_tag="VFX87_10200"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HEX4995332.1"
                     /translation="MSPTEPFVFLGCLELNELLPHEAHDARELLEQLERVPVESVFCH
                     TSASLLHRPVLAEAYPNDFALWVGTEVRDVRLAERLAGVDAFHAGSMERVREELVATI
                     SDHLQHLGGAPARTQGKRFRFFQMHLVPVPTGHQARTLAELRDALVEVDVSALFYHTI
                     EARYRAGRGRGDFAEWVAGALGLPEVAERLAHIDPYAGSLERIRDRHLNILSDAIAEG
                     GA"
     gene            14475..15677
                     /locus_tag="VFX87_10205"
     CDS             14475..15677
                     /locus_tag="VFX87_10205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4995333.1"
                     /translation="MSAGSLLKLDDYRQVAPRGAVDILMRIGERLRGKRFVHVSASRY
                     GGAGVEVLNRLVPIMNDLGVDTTWEITIGTADFDQVGRAVSRALAGTEQVITEPMLAR
                     LRETAAENARRLHLDADLVMVHDATPLGLVEQRSGAGRWVWRCHQDLSSAQPQIWSAL
                     RPIAQRYDAAVFSLARFATPLSIPRFIVYPSIDPLSERNREMTRAEQATQMDRLQVPR
                     DKPILLQVGPFERQHDPLGVINAFRLVKKHHDVRLVLAGPPPTPGGDVLADVQEAAGQ
                     DPDIAVIVLPPDPQTELNALERVATIAIQKPLKADFTIEVATAMWKGKPVIGTIAGGI
                     PFQMVVNVTGYTVETVEGAAFRIRQLLSNPELIGRMGAAGREHVRRNFLVTRQLSDYL
                     ALLAHLTR"
     gene            15677..16912
                     /locus_tag="VFX87_10210"
     CDS             15677..16912
                     /locus_tag="VFX87_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995334.1"
                     /translation="METLELADDVRTTVERIGTADLVVGLATAGPSATVAAVAGAVRA
                     GLDAHFAGQAAVVVHVDQAPSEETPAALASALGPVRMIRAAGTGHAADGALDWAQAVR
                     TMLTVGRRLEAGALVMLNADLGSTTADWVHALATPVLKDEVGLVLPIYQRYRYDGTLT
                     QALVVPLMRSLFGRQLTHPLAEEFACSGAAADAFLAQPIWETDLGRHGLEFWLPAAAI
                     ERDFTVGQAVLGPRTVAVTQPPAPLGRTVGRVAGALFALAEASEHYWLDIRGSEPVVT
                     FGVPPEPLPDGPTVDPSLMQVGFVQGVRDLLPVWERILAPENLGEVLELSDRPLERFR
                     FSDRLWTRVVYDFLLAYRARVVYRSHAAQSLAPLYLGRAASVILETSGRPPAAMLLSA
                     TRLGGMFEEDKPYLVDRWR"
     gene            16909..18891
                     /locus_tag="VFX87_10215"
     CDS             16909..18891
                     /locus_tag="VFX87_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX4995335.1"
                     /translation="MTRIRRPIVIERVRPALDDGRYPVKRVTGDLLNVTADIFKEGHD
                     LLAASIRFRAAGEADWREAPLAPVDNDGWAGSFRLEANSRYRYTVEAWTDAFGSWVEE
                     MRRRLAGGQTDLTSELLEGGAMLRRAREGARDGDAAALARALERLEAASSRDRRLDIL
                     LAPELRQVMARVQPRPDLTRHDRELEVVVDRPAAAFAAWYELFPRSQGRVPGRHGTFH
                     DCADRLPEIQRMGFDVVYLPPIHPIGRTARKGPDNALVAGPGDPGSPWAIGGPEGGHT
                     AVHPELGTLEDFRRLVKTAQGLGMEIALDFAIQCSPDHPWVREHPEWFFPRPDGTIKY
                     AENPPKKYQDIYPINFATPAWESLWDALLGVVRFWIDQGVRAFRVDNPHTKPLDFWTW
                     LIREVQDRDPDVVFLSEAFTRPKVMRALGKAGFTQSYTYFTWRNFKEELTEYLEELTH
                     SEMAEYFRGNFFVNTPDILPEVLQRGGPPAFRMRAALAATLSSVWGIYSGYELCEATP
                     LPGSEEYQASEKYEIRVRDWDAPGSISGYIARLNAIRRENPALHTMTGLRFYPATSPH
                     VLFYGKLAPARDNVILVAINLDPFATHEATLQLPLAEIGIAPDETYELHELMAGDRRL
                     MRGATHTVTLDPRTAPAHVYRVGRWRRRERDFDYYA"
     gene            18899..20578
                     /gene="treS"
                     /locus_tag="VFX87_10220"
     CDS             18899..20578
                     /gene="treS"
                     /locus_tag="VFX87_10220"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX4995336.1"
                     /translation="MSDRDSLWYKDAVFYELHVRAFKDGNDDGIGDFIGLTSQLGHLK
                     ELGVDCLWLLPFFRSPLRDDGYDVADYRAIHPDYGMMADFDRFLDAAHGHGMRVISDL
                     VVNHTSDQHPWFQAARRAPDSPYRDYYVWSDTDERYRDARIIFLDTEHSNWTWDPVAK
                     AYYWHRFFHHQPDLNFDNPQVRRELLDVMRFWLDKGLDGFRCDAVPYLIEREGTSCEN
                     LPETHDFLREIRATIDREYSGRILLAEANQWPADVRPYFGDGDEFHMAFHFPLMPRMF
                     LAVRHGDRRPITDIFVHTPEIPATAQWCLFLRNHDELTLEMVSDEERRHLYYAYGSDP
                     RMRLNLGIRRRLAPLLENDRRKIELMTSLLFTLPGSPILYYGDELGMGDNIYLGDRNG
                     VRTPMQWTGDRNAGFSRADTARLYLPLIVDTVYGYQAINVEAQQRTPSSLLNWMKRLI
                     AVRKKTRVFGRGTLTFLRPANESVLAHAREHEGETVIAVHNLAGSAQPVELDLRAWRG
                     HTPVEMLGDTRFPTIGAQPYFISLAPYGYYWFRLQPPLPDQPLYGIEAAPL"
     gene            20553..>20864
                     /locus_tag="VFX87_10225"
     CDS             20553..>20864
                     /locus_tag="VFX87_10225"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995337.1"
                     /translation="MASRPLHSDPAALAALLSGPGRSDLTRFLIRQRWFAAKTRGIAA
                     VDVLDWAVLDPDGPLVLLLVAVDGDRYYVPATVSAEAAPEATLARAGAEAVVDAHHDP
                     RF"
BASE COUNT         2941 a         7928 c         6964 g         3031 t
ORIGIN      
        1 gcgggcgtga agaggctcgc gtccttccgc ggagtgagcg cggccaggaa cacgaacggc
       61 ttgaacgcgc tgcccggctg gcggcgggcg aggacggccc ggttgaactg gctcgcccgg
      121 tagtcgcggc cgccgacgag cgcgcgaacc tgtccggtgg cggggtcgag cacgatcatc
      181 gccacctgca ggcgctcctc cggcttcttg cggcgcagcc gcggccgcag ggtctcgaga
      241 cggtccatac ccttcaccac tgcggcctcg gccaggcgct gcagcaccgg atcgagcgtg
      301 gtgtagacgc gcacgccgtg cagatcggcc aggtcgatgt cggtgctccg ctcgagctcg
      361 gcgcgcacgt agtccacgaa gtacggcgcc atcagcccgc tcgccggaat ggtccgcgcg
      421 cgcaccggct ccttgcgcgc ccggcggtaa tcggcctccg agatcttgcc gaggtcgcgc
      481 atgcgggtca gcaccacgtc gcgccgctcg cgcgcgcgct cgggattgcc gaccggggaa
      541 tagttgttcg ggccgcggat catccccgcg agcagggcgg cttccggcag cgtgagctgg
      601 tggacctcct tgccgaagta agcccgcgag gcggcgccca ccccgcggac cgccgagccg
      661 gccgattgtc cgagatagat ctcgttcagg taggcctcga gaatctggtc cttgccgtag
      721 cgccactcga gcgcggtcga gaggagcgcc tcgttggcct tgcgggcgag cgtgcgctcc
      781 gggttgagca gccggctctt gacgagttgc tgggtgatgg tgctcccgcc ctcggcgacc
      841 cggcccttgc ggacattggt ccacagcgcg cgcacgatac cgcgggggtc cacgccgccg
      901 tgctcgtaga agcgcacgtc ctcggtgacc aggaccgcgt cgcgcaggat ccgggggacg
      961 tcgggcaggc gcaccgggcg gatgttctcg cccatgtccg cgcccgcgct ggccagcagc
     1021 tcggggggca gggccacgct ctggacctcg gtgccgccct ggcggagctg ggtgatgcgc
     1081 ccgcccgaca cggtcagcgt gacgcgggcc gcgcccgcca cgttgatctc ccacgcgctc
     1141 tcggaccgcg agaactggcc gggcgcaccg gcattgcggg tctcgcgata gccctggcgg
     1201 cccaggatcc ccgccagatc gagcgcgccc acgttgacgc cggatcggag cacgggggga
     1261 gccgcgtaga gcacggtgga gcggcgcgcc tccacccgct cgaaccgcga gagggcgcgc
     1321 gctgcgtaga tggcaagccc accgccgacg agcaccgcga tggccagcag cgcacccgcg
     1381 agaagagtcc accgacgaag gcctcgagac cggccccccc acccgtcgga catcaggcgg
     1441 cctctcctcg cactaggatc cggcacccct ccgcagggtg atcatcacca gccgcgccac
     1501 ctcgggatcg cccgacatct cgatcacgac gaagtcgccc acgcgaacgt cggcgagggc
     1561 gatcgtgctg tcccggaagc tgtccttcgc gtcgcggcca gcctgattgc gctgcgacag
     1621 cagtaccacc gcctcgcgcg gtacctgtac ccggagggcg cgccggtcgg cattggcgcc
     1681 gaactcgtcg agggtcaagg tacgcgcctc ggcatccacc gcgagcaccg agcctgcgcg
     1741 acgctccccc gccggggcca cgcccgcccc catcgccgcg aggcccgcca cgagggcggc
     1801 ccccgccagg agccgggcga gccccgagct cgacacgcgt gcgctcacga ccgcggggag
     1861 gcgggcgccg cgaactcggc cgccttcatc ttccggacca gctcggcgta cgaggaggtc
     1921 tggatgatct tgtcgaactg ggtccggtag ttgttgatca agctcacgcc ctcgacggag
     1981 acatcgtaca cgagccaccg gtctccccgc tgctgcatcc ggtagtccac cgggacctcg
     2041 gtgccctgct tggtcacgaa gcgcgttcgc acggtggcga gcccgccgtc caccgcctca
     2101 cccgcgtacg cgatccgctc gccgctgtac cgctcgatct tcgagatgta ggcccgctcg
     2161 atcagatcgg tgaacagcgc cgtgaactcc tcccggtcct tctcggcgag tccctgccag
     2221 tgccgtccga gggcgcgctt ggccgtctcg ctgaagtcga acacccccgc ggcctccttg
     2281 cggatggcgg cccggcgctc ccgtgcccgg gcctcgggtt tgagcgccgg atcctcgagg
     2341 gtcttgacga tctggtcgac cgaggccttg agctggtcgg tcggggcccc ggcgcgcgcg
     2401 gacgccgggg ccccgaccgt cagccccacc aggacaaccg gaatcagata cgggacgaga
     2461 cgaaggcgca tgcgctaggc cgctcggccg agagcggccg ggtccaccgc gatccggtcc
     2521 tcgatgcgga agtcgggccg gttgcgcccg atctcccgcc tcgccacgtc caccgccgcc
     2581 tctcgcagct ccggccgggg caccgcgccc gtgatctcca cgacgagcgg tgagcctttc
     2641 cagagcggca gatgcgcggt cggcgtcagc ggcaacccgg ccagggtcgc gtccatgagg
     2701 agggcatccg agatcctggc ctgggccgcg atggcctcgt cctcgcgcct gcgcttgagg
     2761 tcatagagct tgaccgcaac tccgaggagc acgaccacga gcagcatcgt tcccaccacg
     2821 atcatttcca tgggcagtca cctcccgggg gcacgaaact gcatcggcca tgccagcggg
     2881 cgcgtccagc cgtcgcttct caagttgctc tcccgataag cccgaagcaa cgcccgcgcg
     2941 acaaagcatc gcgaccgagt aggaatttcc acgtcaatcg tgtaaaggct accagcctcg
     3001 gtgacacgcg cggcgcgtca tcggctcgct tccggcagcg cctggagcgc tgcttcaccg
     3061 gcgagcacct cccgggcgac gtcacccagg cgctggcggg aatccatcgc atgacgccgg
     3121 agccaccgga acgcctcagc ctcgctgagc tggcgatcgc tcatcagctt gcccttggcc
     3181 tggtccacga gcggccgctc ggcgcgcgcc tgctgcagcc ggcgaatctc gcggaaccgg
     3241 gccagcgcca ggtcgagagt gggcccgacc tccgccgcac gcagcggctt gagcatgaag
     3301 cccatcacgc cggcccgatt ggcgtgcttg accacgctct gatcggtgtc ccgcgtgaag
     3361 aggaccgcgg gacagtgcac ctcggcggtg atgcgcggca tcagcgccgg ctcgcgcgcc
     3421 tcggagaggc tcggcgccat gatcacgacg tcgggccggg tttccctcat caccgcgacg
     3481 gcgtcgagcc ggagcggcgc ctcgagcgcc accacgccgc ccgcgtcctc gaccgccgag
     3541 cggagccccg cgcgcgacgc ggaatccgat tccagaatcg ccacccgcca gcgcggcgga
     3601 ccgttgaaca tcctgccgat cgatcgcatg agcgcctccc ttagcagagc gctcgtcgca
     3661 acccgcgtgc cagcgggata tctgatcgcc cggcgatcgg cccggaaaaa tctccccgac
     3721 cggtgccgga aattccttcc tcgctcccgc tgtatccgcg cccgattcgg cgccccgaag
     3781 cacgcaccgg cgccgcgacc gcggtggcac gtcacttgcg tatccattgg agcatgtcac
     3841 tcagattgaa gcgtgaaccc gaggacgaga tctgggacga gcggctgccc gagccgtccg
     3901 acctcctctc ctccgaggag atctcccccg aggacggagt cgaggtcggc gaggagtacg
     3961 accgcgacga cgagaaccgt ggcctcaggc tcctgtctgc tgtgcccggg cccctgatcg
     4021 ccgccgtcac gttcgtccct acgttcctgg ccatcttctt cggactgggc tacctgctcg
     4081 ggggtgccgc tccgaccgga cccacggcga gcgaccccgt cccatcggcg accgcgctcg
     4141 cgtcagaccc cggaacccag ggcacgccgc cgctccccga agcgcttcgc gatccgctca
     4201 ccggcccgcg tatggtcgac tcgctctcgc cgaggccgag cgatccgcgc gagccgctcc
     4261 cggggtcgag cgcggcggac acgccggcgc cggccatcga gtcgaccgga ccgacgctgc
     4321 tgccgccgac gcagccgccc gcggccgtct cctcggcgcc cgagccccgt gtgggtcgac
     4381 cggccgagcc gccccgaccg gcgcaggccg ctgtgctgaa tgccccgaag gagccgacgc
     4441 ctgcggtcga ggctcgcaag agtgggcgcg aatggacgcc cgcagccgca ttcgcggacc
     4501 gggaagccgc ccggcgtctg gccggcagca tccagcagca gggctatcct gtcgagatcc
     4561 ggcaggagca gtcgtcctcc cgcccctggg tggtgtggat cggcgcccag ccccgcggcg
     4621 gcgagcgccg ccgctagccc tccagcccta ccgctgctcc gcctgaagct cccgccaccg
     4681 gcgcgccttg ttgtggagcg tcttcggcgt cgtcccgagg atctcggcgg cgcgggtctt
     4741 gttgttgttc accgactgaa gcgtccgcag aattagctgc ctctcgccgt cgtcgagcgt
     4801 ggtgcccacc ggcagcacca ccacgttggc gcgctcggtg gcctggccgc tcagcggcgc
     4861 gatcggcagg cagttggccg tgatcatccc gtcctcgcac gagaccaccg cacgctcgat
     4921 cgcgttgcgc agctcgcgca cattgcccgg ccaggcgtgt tcccggagca gcccgagcgc
     4981 cgcctcgtcg atcccacgca cctgccgctc gtacttgtcg ttgaactcgg ccatgaacgc
     5041 ttccgcgagg agcgggatgt cctcggggcg gcggcgcagg ggcgggagcg ggaccgtcag
     5101 cacgtttacg cggtagtaga gatcctcccg gaacgtgccg tcctgcaccg ccttcaccac
     5161 gtcccggttg gtcgcgcaga ccacccgcac gtccaccgcg acctcgtcgt ggccgccgat
     5221 ccgccgcacc tggctgtcct gcagcacgcg gaggtacttg gcctgcaggc tcgagctcat
     5281 ctcggcgatc tcgtcgagga agatggtgcc gccgtcggcc agctcgaagt acccgggtcg
     5341 ccgcgcgatc gcgccggtga aggccccctt ctcgtggccg aacagctcgc tctcgagcag
     5401 cgtctccggg atcgccgagc agttcaccgc cacgaacggc cccttggccc gctgcgagag
     5461 ctcgtggatg gcgcgcgcca ccagctcctt gcccgtcccg gtctcgccca ggatcagcac
     5521 gggcgccgga ctcggcgccg ccatctcgat cagccggtag acctcctgca tcgcggggct
     5581 cacgcccagc aggggcccga gcccccgcgt ctccttgagc tggcgccgca gcaccgtgac
     5641 ctcgcggttg acctgaacct tctcgaccgc cttctccacc aggacccgga gccggcgagg
     5701 gtccaccggt ttggtcatgt agtcgtaggc gccttcccgc atcgcggaca ccgcggtctc
     5761 gatggacccg tgggccgtca gcaggatgac ggccgcctcc gggaccgtcg cggccagggg
     5821 cttgagcagc ccgatcccgt cgatcacggg catgaccatg tccgcgacga ccacgtgggg
     5881 gcggaagctt ggcgcccgct ccagcgcgtc cttgccgtcc accgcttcct ggacctcata
     5941 gccccacatg gccagcaggg ccgccagacc gccgcggccg gactcgtcgt cgtctgcgat
     6001 caaaatccgg tgcttcatgg tcgtcctctt ttctggaagc gcacgagggc tccacgatgg
     6061 cttgaaccac cgcggccgtg ggtgcaagca atatgccctc gaaaccggcg gtaatgagct
     6121 gaatcgatgg aaatggaaat tatataacta ggacggtatg ttgtgcagga tatatcaact
     6181 ttcacttgaa agtaatccgc aaaacgagaa ccttgaatca ggaagatatt accgtgtaac
     6241 tatgaccggc tccaatcgac gtcgaccagc gccaacccgg tcgccgggtc ggtcttgcgc
     6301 cggagggtct tgggcgactc gacgatcgtg acctccaggg tcgcgtccac ggttccgccc
     6361 aggaggtgac ccccgagggc gctcccgtcg taccgcgcga ccacgacgtg gacgtgcacc
     6421 cgaggctcgc ccgcgtcgag cgcgacgtta ccgaccagcg agaccacctc gacccgctcc
     6481 cggatgaccc ggcgggcata gtcgggccgg gcccggtcga ggaagccgag agttaccccc
     6541 gagaaggccc cgatcccggt caacgccgcg ccggttagat cgaagcgctt ggcggccacg
     6601 gtgagagcct ccacggcctc gtcgccccgg tcgcagacca tcacgaacac acgccgcccc
     6661 tcgacctcgt cgagctgctg gatcttcatg tcccgctcct ctcgtgagtc atggccgctc
     6721 gatgatccag atgcggcgcg cgcacggcgg ccctgcgccg agcacacatc gcggtccggg
     6781 acgaggcaag ccttgtgcca tgcgcaagaa cggccggccg cgcgctaagc actcgaaccc
     6841 tgcgggcacc ccccgctgcg gattccgggg gacatgcaag agaccggtaa acgttaccga
     6901 tgccgccgac gcgagccgcg cgccctcagg cccggcagcc tttctgcggc cttccacgcg
     6961 tgataccgtg aggcattccc gcaccgcaat agatggaagg aggcgcggat catgacggac
     7021 atgtggggtg atctcgtgtg ggccgcactc gcggatctga agacgaggct ggtcctcgtg
     7081 atgccgggca tcctcgcgat gctgaccctg gccgtggtcg gcctcgccgg cgcgtggatc
     7141 gccgcgcgcg tgacccggcg ggtggcgcag gcggtggcct tcgaccggcg cgccgagaac
     7201 tggggcatcg tcgcgcagtt gagccgtgcg ggcgtgcgcc ggccgccctc gcaggtgctg
     7261 gggctggtcg tcttctggag cattctcgtc ctcttcctgt ccatggccgt cgacgcgctc
     7321 gcgattcccg gcaccgggcg gctcaccgag ttcatgttct cgtgggtccc gcgggtcatg
     7381 ggcgcggccc tgatccttct cgtcggctgg ctcgtggcca acttcctggg cgaaggagct
     7441 ctgattgccg cggtgaacgc gcgcgtcccg gaggcgcgcc tgctcgcccg cgcgattcgc
     7501 tggggcgtgc tgctcttcgc gagcgccacc gcgatcaccc acctgggcat cggcaaggag
     7561 atggtgctcg tggccttcgg gatcaccttc ggcggcctga tcttcgccct ggcgctggcc
     7621 ttcggcctgg ggggccgcgc gctggcgcgc cagatcctcg agcggcacct gcgcaaccgc
     7681 gagccccatc cgcgggagac cctgacccac ctctagcgca tgatcgatgt cgacgtcctc
     7741 ttccagcagg cggcggcggc gctgcggcga gagcgccacg ccacctatcg cctgcagctg
     7801 gggagcacgc tcggcttcga cgcggtggcc gcgctcgggc cgtacctcga ggcgctcgga
     7861 gtgagcgacg cctacctctc cccctgcttc cgctgcggcc ccggcagctc gcacggctac
     7921 gacgtcaccg atcacaattc gttcaatccc gagatcggcg gcgcggccgc cttcgatcgc
     7981 ctggtcgccg cgctcactgc gcgcggcctc ggcctcatcc tcgacgtggt gcccaatcac
     8041 atgggcatcg cgggcgacgc caatccctgg tggctggacg tgctggagaa cggccaggcc
     8101 tccccgcgcg ccggcttctt cgatatcgac tggacgccga tcaagccaga gctgcgcgat
     8161 cgcgtgctcc tgcccgtcct gccggaacag tacggccgcg tgctcgagtc gcagcagctg
     8221 cagctcgagt tcgcggacgg cgcctttcac ctgcgctacg cgggcgcgcg cctgcccatc
     8281 gcccccgaca cctacgcgca gatcctgacc gaccggctcg acgtgctcgc cggccgcctc
     8341 gggcccgagg acgcggggct ccaggagctg cgcagcatcc tgacctcgct cgagcacctg
     8401 cccgggcgga ccgagacgga tcccgcccgg ctcgaggagc ggctgcgcga gaaggagatc
     8461 gtcaagcgcc gcctggccgt tctcaccaag gagtcggcgg acattcgcga gcacgtcgag
     8521 gagacggtgc gcgccgtgaa cggcgccccg ggggatccgg cgagcttcga cgcgctcgat
     8581 cgcctgctct cggcccagac ctaccgcctg gccgactggc gcgtggcggg cgacgaggtg
     8641 aactaccgcc gcttcttcga tgtgagccac ctggcggcca tccgcatgga ggagcgcttc
     8701 gtgttcgacg ccgcccacga gctcgtcttc cggctcatcg gcgagggtaa ggtcaccgcg
     8761 ttgcgggtgg accatccgga cgggctctac gcgcccggcg agtacttccg ccggctgcag
     8821 gagggcgcgc tggtggccac cgcccggcgg ctcctgcccg agctggcccc cgcagaggcc
     8881 gaggcgctcg gcgccctcta ccgcgcccgc gccgccgagc ggccggccgc gccggacgcg
     8941 cggccgctgt ggatcgcggc cgagaagatc ctgagcccgg gcgagcctct gcccgactgg
     9001 tgggcggtgg cgggcaccac cgggtacgac ttcctggcct cggtcaacgg gctcttcgtg
     9061 gatcgaggga cttccaggca gatgaccgcg ctgtacacgc ggctcgccgg ggcgggcggg
     9121 ctgttggccg atcacatgta caccgccaag cggctgatca tgcaggtctc gatggcgagc
     9181 gagatcaacc agctcggcca tcacctcgat tccatgtcgg agcgcaatcg cctctcgcgc
     9241 gacttcaccc tttacagcct ggtgcgcgtg atccgggagc tggtggccgc cttctccgtc
     9301 taccgcacct acgtgggcga cgacgggcgc gatccgagcg tccgcgaccg cgcctatatc
     9361 gaagaggcgg tggcggaggc caagcgccgc aaccccaccg tgaacgtgtc catcttcgac
     9421 ttcgtccgcg acgtgctcct ggtgcggagc ccgagccgtc ccgatcccga cgagcaggcc
     9481 gcccgccggc acttcgcgat gcgcttccag cagacgaccg gaccggtcac cgccaagggc
     9541 gtcgaggaca ccgcgttcta cgtctacaac cgcctcgtct cgctgaacga ggtgggtggc
     9601 gatcccgcgc gctatgggga gtcgccgacc gtcttccacg acaagaacgc ggcgcgcctc
     9661 gcccgctggc cggagtccct cgtcgccacc gccacccacg acaccaagcg cggcgaggac
     9721 gtccgcgcgc gcatcaacgt gctctccgag gtcccggccg cgtgggccac cgaggtccgg
     9781 cgctggcggg ccctcgcccg gcgcttcaag gtggacgtgg acggccgcgc ggcgcccgac
     9841 gccaacgacg agtacctctt ctaccagact ctcgtgggcg cgtggcccgc ccgggacgaa
     9901 gacgagccgc tcgagacgct gaccgagcgc ctcggcgcct acatggagaa ggccaccaag
     9961 gaagccaagc gccacacgag ctgggtgaac ccgagcgccg gctacgacgc ggcgacgcgc
    10021 gggttcgtgg ccgggctgct cgcgcccggc ggggtctttc tctccgcctt ccggcccttt
    10081 cagcgcctgg tcgcggccca cggggcggtc aactcgctcg cccagaccct gctcaagatc
    10141 gtggcccccg ggatccccga cctctaccag ggctgcgagc tctgggatct ctcgctggtg
    10201 gatcccgaca accgccggcc ggtggacttc gcccaccggc gagccctgct cgacaccttg
    10261 cgcgcccgca tccccgccga cgacgcgggc gcgcccgacc tgagcgccct ctgcgcggag
    10321 ctcctcgacc actggcagga cggccgcgtg aagctcttcc tcacccaccg ggcgctcacc
    10381 ctgcggcggc agcgcgcccg gctcttcggc gccggggagt atcgcgggct cgccgggggc
    10441 ggccgccacg ccgaccacgt ggtggcgctg gcgcgcctcg acggggccga cgcggtgatc
    10501 gcggcggtgc cgcgcctgac cgcgcgcctc gccggcctca ccggccgcgt cgcgctgggc
    10561 gaggccgcgt gggagcagac gtgggtcgcg ctcggcgacg agcggctcgc cggcgtctac
    10621 cgcgaccggt tcaccgggct caccgtggcc agcgagcgcc gcgacggcat cgcggccctt
    10681 tccgcgcccg ccctgttccg ccacttcccg gtggcactcc tcgagcgcga aggccggaag
    10741 ccgtgagccc aggcccggcg gcacgccgcg cccatcggat gccgttcggc gcggaggtcc
    10801 gcgaggacgg caccacgcgc ttccgcctct gggcccccgg cgcgcgccgg gtcgacctgt
    10861 ggctcgagga gccggggcgc ccggtggcca tgccgcgcga cgcgggcggg tgggcggagt
    10921 acgtgacgcg ggaagcgccc ccgggcacgc gctaccgcta ccgtatcgac ggggagttcc
    10981 tcgtgcccga tccggccgca cgcttccagc ccggcgacgt gcacggggcg agcgaggtcg
    11041 tggatcccct cgcccatgcc tggacggaca ccgcgtggaa cggcctgccg cgggagcgcc
    11101 tcgtgttcta cgagctgcac gtaggcgcct acacgccggg cggcacgtac gcgggcgtgg
    11161 ccgagcggct cgatcacctc gtctcactcg gcgtcaccgc ggtggagctg atgccgctgg
    11221 ccgacttccc cggccgctgg ggctggggct acgacggcgt gctcctctac gcgcccgagg
    11281 cgacctacgg cccgcccgag gacctcaagg ccctggtgga cgccgcgcac gcgcgcggcc
    11341 tcgcggtctt cctcgacgtg gtctacaacc acttcggccc cgagggcaac tacctgcacc
    11401 gctacgcgcc cggcttcttc aacccgcgcc accagactcc gtggggcgac ggggtcaact
    11461 acgacgggcc cggctcggag gtggtgcgcg cgttcatgat ccacaacgcg ctctactgga
    11521 tcgaggagta tcacctcgac ggcctgcgcc tcgacgcggt caacgccatc gtggacgact
    11581 cgccccgcca cctgctcgtc gagctggccc gcgcggtggc cgacgggccc ggccgcgagc
    11641 gccgcgtgca cctggtgctg gagaacggcg ccaacgaggc gcgctacctg cggcgggaag
    11701 acggtgggcg tccgctctac caggcccagt ggaatgacga cgtccaccac gcgctgcacg
    11761 tgctcctgac cggcgagagc ggcggctact acggcgacta ccagccgccg ttgccgcacc
    11821 tcggtcgctg cctcaccgag ggcttcgcgt tccagggcga gcggtccgtg taccgtgacg
    11881 gcgcgcgcgg cgagccgtgc gcggacctgc cgcccaccgc cttcgtgggt ttcctccaga
    11941 atcacgatca gatcggcaat cgcgccctcg gcgagcggct cgccgcgctc gcccccgtcg
    12001 acgcggtgcg cgcggccacc gcgctgctcc tgctctcgcc cggcctgccc ctgctcttca
    12061 tgggggaaga gtgggccgcg cccgagccgt tcctgttctt cagcgatctc ggccccgatc
    12121 tcggcccgct cgtctccgag ggccggcggc gcgagctcgc ccgcttcccc gagtttgccg
    12181 atcccgcgat gcgcgagcgc atccccgatc cccaggccga gtccaccttc aggcgctcgg
    12241 tcctcgactg gactcacgcg agcgccgccg tccacaagga ctcgctcgcc ttccaccgga
    12301 ccctgctcgc gctgcgggcc cgggccgtcg cgcccctgct cgcggacgag gcggtgccgg
    12361 tcggctggtg gaaagcgctc ggcgacaccg cgctcgaggt cgggtggacg ttcccgtcgg
    12421 gcacgctgcg actcgtcgcc aacctgggcg cggcgccggt cccgcacgag gggccgggcg
    12481 cggactgggg ccgccggctc cacgcgctcg ccctgccgtc gcccgcgtgg tcgacgctac
    12541 cgccgtggag cgtggcgttc tatctggccg gagcgcgccc ccgatgagcg ccaacaggcc
    12601 cggccgccta gtgaggccct ccaccgcgcg cggacttgga atcaatcgct tcacggtaga
    12661 ccgtcatggt ccgatccgcg atgcgatccc acgagaagtg ggcctccacc cgccggcgcc
    12721 cggcctcgcc catgcgccgg gcgcgctcgg gatcggccag cgtctcccgg agggcctggc
    12781 cgagcgcggc cgcgtcgccg ggctccagca gccggccggt ctcgccgtcc accaccacct
    12841 cggggatacc cccgacccgg ctcgccacca ccggcgtgcc gcaggccatg gcctcgaggt
    12901 tgatcaggcc gaacggctcg tagatggagg ggcagacgaa gaccgcggcg tggctgtaga
    12961 gctgcacgac ctcgggcacc ggcagcatcg cgttgatcca gcggatctcc gggcggcccg
    13021 ccaccgcggc ctcgagccgg gcgagcagct ccggcgtgtc cgggctgctc gcgcagagca
    13081 cgagctggac gcccgcgggc agggcgcgcg cggcctccag gagctggaag atccccttct
    13141 gctcgctgat ccggccgacg aagagcacgt agggcgctcg caccccgtgg cgcgcgaggg
    13201 cgtcgcggcg ctcggtgcgc gcgaacgcct ccgcgtccac cccgttgtgg atcaccacca
    13261 cccgcgcggg atcgacctgg aagtgcgtca tgatgtcggc gcgcatctgc gccgacaccg
    13321 cgatgacgcg gtcggcacgc tcgacggcca gccgctcggc ccagctcgac accgcgtacc
    13381 cggtgccgag ctgatcggcc ttccacggcc ggagcggctc catgctgtgg agcgtgacga
    13441 cgagcggcac gtcgtggatg gactgcacga gcatcccgcc cagccccaca taccacgtat
    13501 gcgtgtggac cacgtcggcc gccaccggat cgcgcgccat ggcgaggccc accgagagcg
    13561 cctcgagcgc gggcgcgtag ggcgcctccg gcccacccgc cagccgcggc cacggcgcgt
    13621 agccccggac ggtcagaggc ggccgcgcct gctcctgctc gccgaagcag cgcacctcga
    13681 ccgacgcgca ccgggccagc gcgcggctca ggtgatccac caccacgccg gcgccgccgt
    13741 agacgtgcgg cgggtattct cggctgagca gcaggattct cacaggcgtc gtcccccggg
    13801 agagcttacc gcatgagccc gaccgagccc ttcgtcttcc tcggctgcct cgagctgaac
    13861 gagctgctgc ctcacgaggc ccacgacgcc cgcgagctgc tcgagcagct cgagcgcgtg
    13921 ccggtggagt ccgtcttctg ccacacctcg gcctccctgc tgcaccggcc ggtgctggcc
    13981 gaggcctatc ccaacgactt cgcgctctgg gtcggcaccg aggtgcgcga cgtgcggctc
    14041 gcggagcgcc tggccggcgt ggacgccttc cacgcgggct ccatggagcg cgtgcgtgag
    14101 gagctggtcg ccaccatctc cgatcacctc cagcatctcg gcggagcgcc cgcccgcacc
    14161 cagggcaagc gcttccgctt cttccagatg cacctcgtcc cggtgcccac cggccaccag
    14221 gcccgcacgc tggccgagct gcgcgacgcc ctggtcgagg tggacgtgag cgcgctcttc
    14281 taccacacca tcgaggcgcg ctatcgcgcc ggccgtgggc gcggcgactt cgcggaatgg
    14341 gtcgccggcg cgctcggcct ccccgaggtg gccgagcgcc tcgcccacat cgacccgtac
    14401 gcgggcagcc tcgagcggat ccgggaccgc catctcaaca tcctgtccga cgccatcgcg
    14461 gaaggaggcg cttgatgagc gccggatcgc tgctcaagct cgacgactac cgccaggtcg
    14521 ccccgcgcgg cgcggtggac atcctcatgc gcatcggcga gcgcctgcgc gggaagcgct
    14581 tcgtgcacgt gagcgcctct cgctacggag gcgcgggggt cgaggtgctg aaccggctcg
    14641 tgccgatcat gaacgacctg ggcgtggaca ccacctggga gatcacgatc ggcaccgccg
    14701 acttcgacca ggtcggccgc gcggtgagcc gggccctcgc gggcaccgag caggtcatca
    14761 ccgaaccgat gctggcccgc ctccgcgaga ccgcggccga gaacgcgcgc cggcttcacc
    14821 tcgacgccga tctcgtgatg gtgcacgacg ccaccccgct gggcctcgtc gagcagcgca
    14881 gcggcgccgg gcgctgggtg tggcgctgcc accaggatct ctcctccgcc cagccgcaga
    14941 tctggagcgc gctccgcccg atcgcccagc gctacgacgc ggcggtcttc tctctcgccc
    15001 gcttcgcgac gccgctctcc atcccgcgat tcatcgtgta tccctccatc gatccgctct
    15061 ccgagcgcaa ccgcgagatg acccgcgccg agcaggccac ccagatggac cgcctccagg
    15121 tgccgcggga caagcccatc ctgctccagg tggggccgtt cgagcgccag cacgacccac
    15181 tcggtgtgat caacgccttc cgtctcgtga agaagcacca cgacgtccgc ctcgtgctcg
    15241 ccggcccgcc ccccaccccc ggcggggacg tcctcgccga cgtacaggag gcggccggcc
    15301 aggatccgga catcgcggtg atcgtgctgc ctccggatcc ccagaccgag ctgaacgcgc
    15361 tggagcgcgt ggcgaccatc gcgatccaga agccgctcaa ggccgatttc accatcgagg
    15421 tggcgaccgc gatgtggaag ggcaagccgg tgatcggcac catcgcgggc ggcatcccat
    15481 ttcagatggt cgtgaacgtc accgggtata ccgtggaaac cgtggagggc gccgcgttcc
    15541 gcatccggca gcttctgagc aaccccgagc tcatcggccg tatgggcgcg gccgggcgcg
    15601 agcacgtccg ccgcaatttc ctcgtcaccc gccagctgag cgactatctc gcgctgctcg
    15661 cccatctcac ccggtgatgg aaacgctcga gctcgccgac gacgtccgga ccaccgtcga
    15721 gcggatcggc accgccgacc tcgtggtggg cctggccacc gccgggccga gcgcgaccgt
    15781 cgcggcggtg gccggcgcgg tacgggcggg cctcgacgcc cacttcgcgg gccaggccgc
    15841 ggtggtcgtc cacgtggacc aggccccctc cgaggagacg ccggccgccc tggcctctgc
    15901 cctgggcccc gtgcgcatga tccgggccgc cgggaccggc cacgccgcgg acggcgcgct
    15961 cgactgggcc caggcggtgc gcacgatgct gaccgtcggc cggaggctcg aggcgggtgc
    16021 cctcgtgatg ctcaacgccg atctcggcag caccaccgcg gactgggtcc acgccctggc
    16081 gacgcccgtg ctcaaggacg aggtcgggct ggtgctgccg atctaccagc gctaccgcta
    16141 cgacggcacc ctcacccagg ccctcgtcgt gcccctcatg cgcagcctct tcggccgaca
    16201 gcttacgcat ccgctggccg aggagttcgc ctgctcgggg gcggccgccg acgccttcct
    16261 cgcgcagccg atctgggaga cggacctcgg ccgtcacggc ctcgagttct ggctgcccgc
    16321 agccgcgatc gagcgggact tcacggtggg gcaggccgtg ctgggcccgc gaacggtggc
    16381 ggtcacccag ccacccgccc cgctcggccg cacggtgggc cgggtggccg gcgccctctt
    16441 cgccctggcc gaggcgtcgg aacactactg gctggacatc cggggatcgg agccggtcgt
    16501 gaccttcggc gtgccccccg agccgctgcc ggacggtccc acggtggatc cgtcgctgat
    16561 gcaggtcggc ttcgtccagg gtgtgcgcga cctcctgccg gtgtgggagc gcatcctggc
    16621 ccctgagaat ctgggcgagg tgctggagct cagtgatcgg ccgctcgagc gattccgctt
    16681 ctccgaccgc ctctggacgc gcgtggtcta cgacttcctg ctcgcctatc gcgcccgcgt
    16741 cgtctaccgg agccacgccg cccagtcgct ggccccgctc tacctgggcc gcgccgcctc
    16801 cgtgatcctc gagaccagcg ggcggccccc cgccgccatg ctcctgagcg cgacgcgcct
    16861 cggcggcatg ttcgaggagg acaagcccta tctggtggac cggtggcgat gacccgtatc
    16921 cgtcgcccca tcgtgatcga gcgcgtgcgc cccgcgctgg acgacggtcg gtatcccgtc
    16981 aagcgcgtca ccggggatct cttgaacgtc accgccgaca tcttcaagga gggccacgac
    17041 ctcctcgccg cgtccatccg cttccgggcc gccggtgagg ccgactggcg cgaggcgccg
    17101 ctcgcgccgg tggacaatga cggctgggcc gggtcgttcc ggctcgaggc caactcgcgc
    17161 tatcgttaca cggtggaggc gtggaccgac gccttcggct cctgggtcga ggagatgcgg
    17221 cgacgcctgg ccggcggcca gaccgatctc acgagcgagc tgctggaagg cggcgcgatg
    17281 ctccgccgcg cccgcgaggg cgcgcgggac ggggacgcag ccgcgctggc ccgcgcgctc
    17341 gagcggctcg aggccgcgtc ctcgcgcgac cggcgtctcg acatcctcct tgcccccgag
    17401 ctgcgccagg tgatggcgcg cgtccagccg cgtcccgacc tcacccgcca cgaccgggag
    17461 ctcgaggtgg tggtggaccg gccggccgcc gccttcgcgg cgtggtacga gctcttccca
    17521 cgctcgcagg gccgcgtccc cggccgccac ggcaccttcc acgactgcgc cgaccggctc
    17581 cccgagatcc agcgcatggg cttcgacgtc gtctacctgc cgcccatcca tccgatcggg
    17641 cgcaccgccc gcaagggccc cgacaacgcg ctcgtcgccg gccccggcga tccgggtagc
    17701 ccctgggcca tcggcggccc cgagggcggg cacaccgcgg tgcatccgga gctcggcacg
    17761 ctcgaggact tccgccgcct cgtgaagacc gcgcagggcc tggggatgga gatcgcgctg
    17821 gacttcgcga tccagtgctc gccggaccac ccgtgggtgc gcgagcatcc ggagtggttc
    17881 ttcccgcggc ccgacgggac catcaagtac gcggagaacc cgcccaagaa gtatcaggac
    17941 atctacccga tcaacttcgc gacgccagcc tgggaatcgc tctgggacgc gttgctcggc
    18001 gtcgtccggt tctggatcga ccagggcgtg cgggcgttcc gcgtggacaa cccccatacc
    18061 aagccgctcg acttctggac ctggctgatc cgggaggtcc aggatcgcga tcccgacgtc
    18121 gtgttcctct cggaggcctt cacccgcccg aaggtgatgc gggcgctcgg caaggccggc
    18181 ttcacccagt cctacacgta cttcacctgg cgcaacttca aggaagagct gaccgagtat
    18241 ctcgaggagt tgacgcactc cgagatggcc gagtacttcc gtggcaactt cttcgtcaac
    18301 acgcccgaca tccttcccga ggtcctgcag cgcggggggc cgcccgcttt ccgaatgcgg
    18361 gccgccctgg ccgccacgct atcctcggtc tggggcatct acagcggcta cgagctctgc
    18421 gaggcgacgc ccctgcccgg cagcgaggag taccaggcct cggagaagta cgagatccgg
    18481 gtgcgcgact gggacgctcc cggcagtatc tcgggctaca tcgcgcggct caacgcgatc
    18541 cggcgcgaga accccgcgct ccacaccatg accggcctgc gcttctaccc ggcgaccagc
    18601 ccgcacgtcc tcttctacgg caagctcgcc cccgcccgcg acaacgtgat cctcgtcgcg
    18661 atcaacctgg atcccttcgc cacccacgag gccacgctcc agctcccgct cgccgagatc
    18721 ggcatcgccc ccgacgagac ctacgagctc cacgagctga tggccggcga ccgccgcctg
    18781 atgcgcggcg ccacccacac cgtcaccctc gacccgcgca ccgcccccgc ccacgtctac
    18841 cgcgtgggcc gctggcgccg ccgcgagcgc gatttcgact attacgcgtg aggcgcgagt
    18901 gagcgaccga gacagcctct ggtacaagga cgccgtcttc tacgagctgc acgtccgcgc
    18961 cttcaaggac ggcaacgacg acggcatcgg cgacttcatc gggctcacca gccagctcgg
    19021 gcatctcaag gagctcggcg tggactgcct gtggctcctg cccttcttcc gctcgcccct
    19081 gcgcgacgac ggctacgacg tggccgacta ccgcgcgatc cacccggact acgggatgat
    19141 ggccgacttc gatcgcttcc tcgatgcggc ccacggccac ggcatgcggg tcatctcgga
    19201 tctggtggtc aaccacacct cggaccagca cccgtggttc caggccgccc ggcgcgcgcc
    19261 cgactctccg taccgcgact actacgtctg gagcgacacc gacgagcgat accgggacgc
    19321 ccgcatcatc ttcctcgaca ccgagcactc caactggacc tgggatccgg tggccaaggc
    19381 ctactactgg caccgcttct tccaccacca gccggatctg aacttcgaca acccgcaggt
    19441 ccgccgcgag ctgctcgacg tcatgcgctt ctggctggac aagggcctcg acggcttccg
    19501 ctgcgacgcg gtcccctatc tcatcgagcg tgaaggcacc agctgcgaga acctgcccga
    19561 gacccacgac ttcctgcgcg agatccgcgc aaccatcgac cgcgagtact ctgggcgtat
    19621 tctgttggca gaagccaacc agtggcccgc cgacgtccgc ccctacttcg gcgacggcga
    19681 cgagttccac atggccttcc acttccccct gatgccccgc atgttcctgg ccgtccgcca
    19741 cggggaccgg cgcccgatca ccgacatctt cgtgcacact ccggagatcc ccgccaccgc
    19801 gcagtggtgc ctcttcctgc gcaatcacga cgagctcacc ctcgagatgg tgagcgacga
    19861 ggagcgacgg cacctctact acgcctacgg ctccgacccg cgcatgcggc tgaacctcgg
    19921 catccggcgc cggctggccc cgctgctcga gaacgaccgg cgcaagatcg agctgatgac
    19981 gagcctgctc ttcacgctgc ccggcagccc gatcctctac tacggcgacg agctcggcat
    20041 gggcgacaac atctacctgg gcgaccgcaa cggggtccgc accccgatgc agtggacggg
    20101 cgaccgcaac gcgggcttct cccgcgcgga caccgcgcgc ctctacctgc ccctcatcgt
    20161 ggacacggtg tacggctacc aggccatcaa cgtggaggcc cagcagcgca ccccgtcctc
    20221 gctgctcaac tggatgaagc ggctcatcgc ggtgcgcaag aagacgcggg tcttcggccg
    20281 cggcaccctc accttcctcc gcccggccaa cgagagcgtg ctcgcccacg cccgcgagca
    20341 cgagggcgag acggtgatcg cggtgcacaa cctcgccggc tcggcccagc cggtggagct
    20401 cgacctgcgc gcctggcggg gccacacccc ggtggagatg ctgggcgaca cgcgcttccc
    20461 caccatcgga gctcagccct acttcatcag cctggcgccc tacggctact actggttccg
    20521 gctgcagcca cccctgcccg accagccact ctatggcatc gaggccgctc cactctgatc
    20581 ccgccgcgct cgccgcgctg ctctcgggcc ccggccgctc cgatctcacg cgcttcctga
    20641 tccgccagcg ctggttcgcg gccaagactc gcggcatcgc ggcggtggac gtgctcgact
    20701 gggccgtcct cgacccggac gggccgctcg tgctcctgct cgtcgcggtg gacggtgacc
    20761 ggtactacgt cccggctacc gtgtcggccg aggccgcgcc ggaggccacg ctcgcccgcg
    20821 ccggcgcgga ggcggtggtg gatgcccacc acgacccgcg attc
//
LOCUS       DASUFA010000274        12342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_509485, whole genome shotgun sequence.
ACCESSION   DASUFA010000274 DASUFA010000000
VERSION     DASUFA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12342
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509485"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VFX87_10230"
     CDS             complement(<1..1039)
                     /locus_tag="VFX87_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4995338.1"
                     /translation="MTRRGLLALVLLGAAALLPGGCGGDKPTSIKVVIAEYSRDHTRP
                     FWQGLAEQYTKQTGVKVDLQVIDWNSIDQQVSTMIQNNQPPDVLNLNSFSSYAKDGLL
                     YSGDEALSPTTRDDFLEAFARGGTYQGKLYGFPILASARAFFYNRDLLAKAGVAAPPR
                     TWDELVQAARRVQALGGGAIGYALPLGPEEAQAEWSIWMWNNGGDWKAGEAWAINSEK
                     NVQTLQFLAALANTHKVTQINPGKTNRTDGAFQLFKDGKVGMVIGFSPLAAQLDAEGK
                     VRYGVTQMPTNVGTPATLGVEDYLMAFKKKGNQEAVRRFLDLYYQPEQITRWITAEGF
                     LPVTKSGRERMS"
     gene            1155..1571
                     /locus_tag="VFX87_10235"
     CDS             1155..1571
                     /locus_tag="VFX87_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995339.1"
                     /translation="MSMSRVLIVCTLVLALTSCASSSGPARQRSEFEDVPVPAGLTLD
                     QNRTTIIESPTVKAARLIYKSRIKPESLALAYRASLEANGWRHVSSTTSSSKGTTQVY
                     EKQDSSLQIMIYEGWYYTWTEVSTTRIVGRPAAPSR"
     gene            1714..1896
                     /locus_tag="VFX87_10240"
     CDS             1714..1896
                     /locus_tag="VFX87_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995340.1"
                     /translation="MAPTRTHVWVPGQWSWHRERYVWVPGHWEVPPAPGHIWVPGGWM
                     PREGTYVWVEGHWRAR"
     gene            complement(1970..3334)
                     /locus_tag="VFX87_10245"
     CDS             complement(1970..3334)
                     /locus_tag="VFX87_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4995341.1"
                     /translation="MIPDRLRQVRLVLRVLLIAVVLALPGAATAQQPVQTPLLLMISI
                     DGLRPDYVIAADTHGAKVPNLRRFLKEGAYAQGVVGVIPTVTYPSHTTLITGVSPATH
                     GIFANITFDPLRENQQGWYWYTEDIRVATLWDAASRAGLSTASVQWPVSVGARVTWNI
                     PEFWRSGGPDDAKLLRAVSTPGLLAELEPELGPYPRALTVESDEQRARYAARILEKKR
                     PNLMTLHLIALDHVQHVTGPFSAETMAVLERLDTLVGTLRTTAETVAPEGAHVAIVSD
                     HGFGKTAMELNLYAELRRAGLVTVSGGKVADWKAMPWTAGGAIAVVLKDPGDAATLAT
                     VRALLDRLAADPAHGIDRVLDAAALRIRGGFPTAAFVVGLKPDWHAGSSLNGPLVGKA
                     TMAGMHGHLPDLPDQHAAFFVVGPGVPAGRTLGLIDMRDIATTLARRLNLALPAAEGK
                     ELWP"
     gene            3393..3758
                     /locus_tag="VFX87_10250"
     CDS             3393..3758
                     /locus_tag="VFX87_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995342.1"
                     /translation="MAGALLMMTATLAGPAAAQDAEKIAMARVRLTTEPGLTQGCTRI
                     GPARDDSIKDLRRKIVKMGGNTALMAFGGTEDLSMVYAEVFRCGDPPAAAPGTAPARV
                     PLPPAGPPPPPPPPPAPTR"
     gene            3967..4953
                     /locus_tag="VFX87_10255"
     CDS             3967..4953
                     /locus_tag="VFX87_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995343.1"
                     /translation="MGARGRPLTRWCLVLALPAIAWFPSIADSAEPEVGGMITEIRLG
                     QGRVEVGSAGGERHRPATPLLTLRDGDVVATTGDAWVIVVLSGGRGAVRVDEATSPFV
                     VTAPPPARGQLQKGLMMLEASFDFLSTTSKDAPFGNLGSRAGVRPPVILTPRNGPVLP
                     DSLVFEWRGSQSSRYVVRIVTADDVVIERADLAGTRFEYPKDAPRLVAGVRYRFQLLP
                     AGQLPQEVWFEVVDSARARTIQHDLREMDAALPGGPSDTTAAILRAGFLAQHGLLHDA
                     RLRVADALARRPDEPTLYFLLGELSARQGLPNEAMEAFAEARFLLSAPATPR"
     gene            4997..5764
                     /locus_tag="VFX87_10260"
     CDS             4997..5764
                     /locus_tag="VFX87_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4995344.1"
                     /translation="MAWDPAQYLKFADHRLRPAIDLLNRIALEHPGDVYDLGAGAGNV
                     TRLIKERWPEARVTGVDDSPAMLAKAAATAPAITWQQADLATWSPPRPADLIYSNAAL
                     HWLTGHERLFPALLAALAPAGVLAVQMPRNFGAPSHTLISEAARGGPWRAKLELLLRP
                     APVADPDFYYDALAPRAAALDIWETEYLQILEGADPVKEWTKGTWLKPLLDALEEPER
                     SRFETRYAELVARAYPPRADGRTLFPFRRLFIVARAR"
     gene            5773..6900
                     /gene="menC"
                     /locus_tag="VFX87_10265"
     CDS             5773..6900
                     /gene="menC"
                     /locus_tag="VFX87_10265"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX4995345.1"
                     /translation="MKIDAIDVYWMKLPLAFVWKTSYADQYFTDTILVRMEGGGHEAW
                     GESCPVFIPAYSAEHTLATFHTLREHMVPRIIGQDIETAQDLLDRLAFIKGNQFARAA
                     LDIAWWVLDAKRRGLPLHRALGGTADRVTVGADFGIQDSLDILMAKIQGAIDAGFPRV
                     KLKFRPGWDLFMVEAVRSTFPDFTFHVDCNAAYTPADTELFRKLDRFGLAMIEQPLAD
                     DGMSLVNHADLQQRIGTPVCLDESAQSLAHVQAAIRLKSCRVVNIKMARVGGLTESRE
                     IQALCAQHDIPCWVGGMLESAIGGAICAELATLPNFTYAGDIFPSSYFYANDLGKPEI
                     VLSGPGEVATSRVPGIAQAPDPELLERWTAEHATFRPASTR"
     gene            7072..7266
                     /locus_tag="VFX87_10270"
     CDS             7072..7266
                     /locus_tag="VFX87_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995346.1"
                     /translation="MNVRENAAKIATQFEGRCTCAAMDGGVACPWCQVFHDVLQGHPI
                     TPPPARRGDTATPRLAPTAA"
     gene            complement(7272..8042)
                     /locus_tag="VFX87_10275"
     CDS             complement(7272..8042)
                     /locus_tag="VFX87_10275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4995347.1"
                     /translation="MAQWDRLDVDGSPMRLYVGMPSLGRAFPAVIVIHHGPGVDKFIE
                     DRVENLAQQGYLAIAPDLYHRQTSAAADASSAAAGVSADADMMTRIGRLRDPEVIADV
                     NAAVNYARRLKDTQLGDVGIIGFCMGGRVAYLMAASKPVFKAAGVFYGGNIMKAWGNG
                     PTPFDLTPYIHCPIAGFFGADDANPSPADVDRISAQLDKYRKAHEFHRYPDAGHAFLN
                     FTNPATYRDGPARDAWDKLIPFLQRNLQTSRQPRQRLT"
     gene            8378..8806
                     /locus_tag="VFX87_10280"
     CDS             8378..8806
                     /locus_tag="VFX87_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995348.1"
                     /translation="MRRWLAALAPIALLVLGPAGAWAADRTMGSQGAGQGGGQGSGHG
                     SGMTRPHSRPPRPIVRPHSVIGCCLGGVSFPPAAPPVVVSLPPPVYVIVPSPPSLVFV
                     PTPRVEPAPEVTLPTGRWARHGNGVDYPYVWVWEGPARGR"
     gene            complement(8831..8983)
                     /locus_tag="VFX87_10285"
     CDS             complement(8831..8983)
                     /locus_tag="VFX87_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995349.1"
                     /translation="MQKKYGFARRYRYNRHAERAGPSRLGQLKLESERQGIGRSNRRN
                     GYVVRA"
     gene            9146..9916
                     /locus_tag="VFX87_10290"
     CDS             9146..9916
                     /locus_tag="VFX87_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995350.1"
                     /translation="MARANLNGIEIDYEATGAGPAVFLSHGYSATRRMWDDQHRALGD
                     RYRVISWSMRGHGATESPADPARYSAPLTIADMKALLGHLGVERAVIGGLSLGGYVSL
                     GFALEYPQMTRALVICDSGPGYRSADAREAWNARARERAVSLETRGLDALGQSREVQE
                     SASLHRSAQGLAHAARGMLAQEGSRVIDGLAGIRVPTLIIVGDQDQPFLAPCEYMAKK
                     IPGARLEVIRGAGHSSNLDQPEAFNRVLLHFLDGLPPA"
     gene            complement(9924..10541)
                     /locus_tag="VFX87_10295"
     CDS             complement(9924..10541)
                     /locus_tag="VFX87_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX4995351.1"
                     /translation="MIYEIRTYRVKVGSLPEVEKRFGEAYEHRKKISPLAAFWHTEIG
                     PLNEIIHVWPYADLGERNRLRAEAVKAGNWPPKIAEFIETMQSEIVVPFAFLPDLKPG
                     KMGPFFEMRYYAMKPGTLPDLIKRWESKIEERVKMSPVALAGHVEHGEANRFIHIWAY
                     KSLDERTAVRNKAREMGVWPPPGGGDTLLTMANKIMLPSAFSPVQ"
     gene            complement(10595..11347)
                     /locus_tag="VFX87_10300"
     CDS             complement(10595..11347)
                     /locus_tag="VFX87_10300"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4995352.1"
                     /translation="MRLAGKVALISGGARGMGASEAILFAREGARVVLGDILDAEGKR
                     VEAEIAAKGGEAVYVRLDVTSEPDWERAVATAVGRFGRLDVLVNNAGVGGGSLLEHTT
                     AEAWDHGMAVNAKGVFLGTKAAIPAMRRSGGGSIVNISSQLGIVGLERSSPQYQASKG
                     AVRTLTKLTAVQYARERIRANSVHPGPIVTPMTEARRGDPGHYEYMVSRIPLGRYGEP
                     DEVAYGVLYLASDESSFVTGSELVIDGGWTAQ"
     gene            complement(11344..11973)
                     /gene="upp"
                     /locus_tag="VFX87_10305"
     CDS             complement(11344..11973)
                     /gene="upp"
                     /locus_tag="VFX87_10305"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX4995353.1"
                     /translation="MDDVVVIGHPLVQHKLGLMRKRETSTSEFRSLLAEISMLLAYEV
                     TRELPTHEVAAETPVGPTTIRLLDGKKIVLISILRAGNGLLDGMLRILPSARVGHVGL
                     YRDPKTLEAVEYYFKVPHEMAERDVVVLDPMLATGNSAVAAVNRVKATAPRSIRFVCL
                     LTCPEGLEHFHQAHPDVPVYTAAIDSHLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(12106..>12342)
                     /locus_tag="VFX87_10310"
     CDS             complement(12106..>12342)
                     /locus_tag="VFX87_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4995354.1"
                     /translation="TRPELVYTHEWRVGDLVMWDNRCMLHRGRPWDESKYRRVMHRTT
                     VAGEGPTAPDEGPAEVSPAREVDMTWGRSQLELV"
BASE COUNT         1742 a         4424 c         4309 g         1867 t
ORIGIN      
        1 cgctcatgcg ctcgcggccc gacttggtca cgggcaggaa gccctcggcg gtgatccagc
       61 gggtgatctg ctcgggctgg tagtacagat cgaggaaccg gcgcaccgcc tcctggttgc
      121 ccttcttctt gaaggccatc aggtagtcct cgaccccgag cgtcgccggc gtccccacgt
      181 tggtcggcat ctgggtcacg ccgtagcgga ccttcccctc ggcgtcgagc tgggcggcga
      241 gggggctgaa gccgatgacc atgccgacct tgccgtcctt gaagagctgg aaggcgccgt
      301 cggtgcggtt ggtcttgccc ggattgatct gggtcacctt gtgggtattg gcgagcgcgg
      361 ccaggaactg gagggtctgg acgttcttct cgctgttgat ggcccacgcc tcgccggcct
      421 tccagtcgcc cccgttgttc cacatccaga tggaccactc ggcctgcgcc tcctccggtc
      481 cgagcggcag cgcgtagccg atcgccccgc cccccagcgc ctgcacccgc cgggccgcct
      541 ggacgagctc gtcccaggtc cgcggcggag cggccacgcc ggccttggcg agcaggtcgc
      601 ggttgtagaa gaaggcgcgc gcgctggcca ggatcgggaa tccgtagagc ttgccctggt
      661 aggtgccgcc ccgcgcgaag gcttcgagga aatcgtcccg tgtcgtgggc gacagcgcct
      721 cgtcgcccga gtagaggagg ccgtccttgg catagctcga gaacgagttc aggttgagca
      781 cgtcgggcgg ctggttgttc tggatcatgg tgctgacctg ctgatcgatg gagttccagt
      841 cgatcacctg caggtccacc ttcaccccgg tctgcttggt gtactgctcg gccaggccct
      901 gccagaacgg gcgcgtgtga tcgcggctgt actccgcgat caccaccttg atggacgtgg
      961 gcttgtcgcc gccgcagccg cccgggagga gcgcggcggc gccgaggagg acgagggcca
     1021 ggagaccgcg gcgcgtcatc gagcccatac tacaaaagtc cggccgaggg cgtaagtctt
     1081 ccgcacgacc cggccgcgca tgcttagaat aggacgtcag actactcgcg gccccaaaat
     1141 gggaggtcac ctccatgtca atgtcccgtg tcctcatcgt ctgcacgctc gtgctcgcgc
     1201 tgacgtcgtg cgcctcctcg tccggaccgg cgaggcagcg cagcgagttc gaggacgttc
     1261 cggttccggc gggcctcacc ctggaccaga accggaccac catcatcgag tcgccgaccg
     1321 tgaaggcggc ccgcctcatc tacaagagcc ggatcaagcc cgagagcctc gcgctcgcct
     1381 accgcgcctc gctcgaggcc aacggctggc gccacgtgtc ctccaccacg tcgtcctcca
     1441 agggcacgac ccaggtctac gagaagcagg acagctcgct gcagatcatg atctacgagg
     1501 gctggtacta cacgtggacg gaggtctcca ccacgcggat cgtcgggcgc ccggccgccc
     1561 cgagccgcta ggcacgcggg aggtcgcgcc gtcttcacgc gggcgctagc ggccacggtc
     1621 gtcgcgctgc tcctggccgg ctgcgcggcc acccagcccg gcgtggtcaa gcgcccgccg
     1681 ccgcagccgc cgcgccgcgc cgagatcgcg ccggtggctc ccacgcgcac gcacgtctgg
     1741 gtgcccggcc agtggtcgtg gcatcgcgag cgctacgtgt gggtgccggg gcactgggaa
     1801 gtgccgcccg caccggggca catctgggtg ccgggcggct ggatgccgcg cgagggcacc
     1861 tacgtctggg tggagggcca ctggcgcgcc cggtgagggg gcgcaccctc tgctcccatc
     1921 ggggggacgc ggatcggcac ctgcgcgagc gagaccgact tcatgggctt catggccaga
     1981 gctccttgcc ctccgcggcg ggcagcgcaa gattcagacg gcgcgcgagc gtcgtcgcga
     2041 tgtcacgcat gtcgatgagc ccgagcgtgc ggccggccgg cacgcccggc ccgaccacga
     2101 agaaggcagc atgctggtcg ggcaggtccg gcagatggcc atgcatgccc gccatggtcg
     2161 ccttgccgac cagcgggccg ttcaggctcg agcccgcgtg ccagtccggc ttgagcccga
     2221 cgacgaacgc ggcggtggga aatcctccgc ggatacggag cgcggccgcg tccagcacgc
     2281 ggtcgatgcc gtgcgcgggg tcggcggcca ggcggtcgag cagggcgcgc accgtcgcga
     2341 gggtggcggc gtcgcccgga tccttcagca ccaccgcgat cgcgccgcca gccgtccacg
     2401 gcatcgcctt ccagtcggcg accttgccgc cgctcaccgt gaccagcccc gcccggcgga
     2461 gctcggcgta caggttcagc tccatcgcgg tcttgccgaa gccgtggtcc gaaacgatgg
     2521 cgacgtgggc accctccggc gccaccgtct ccgcggtggt cctgagcgtg cccaccaggg
     2581 tgtcgaggcg ctcgagcacc gccatggtct cggcgctgaa cggccccgtg acgtgctgca
     2641 cgtggtcgag cgcgatgaga tggagcgtca tgagattcgg ccgcttcttc tcgaggatgc
     2701 gcgcggcgta ccgcgcgcgc tgctcgtccg actccacggt gagcgcgcgc ggataggggc
     2761 ccagctccgg ctcgagctcg gcgagcagcc ccggagtgga caccgcgcgc agcagcttgg
     2821 cgtcgtcggg cccgcccgac cgccagaact ccgggatgtt ccaggtcacg cgcgcgccca
     2881 cgctgaccgg ccactgcacg ctggcggtgc tgagcccggc gcgcgacgcc gcgtcccaga
     2941 gggtggccac ccggatgtcc tcggtgtacc agtaccagcc ctgctggttc tcgcgcagcg
     3001 ggtcgaacgt gatgttggcg aagatcccgt gggtggccgg cgacacgccg gtgatgagcg
     3061 tggtgtggct cgggtacgtg acggtcggga tcacgcccac cacgccctgg gcgtaggcac
     3121 cctctttcag gaagcggcgc aggttcggga ccttcgcgcc atgcgtgtca gcggcgatca
     3181 cgtagtcggg ccggaggccg tcgatcgaga tcatgaggag cagcggggtc tgcaccggct
     3241 gctgagcggt cgcagcaccg gggagcgcga gtaccaccgc gatcaggagc acccgcagca
     3301 cgagcctgac ctggcggagc cggtcgggta tcatggcgaa cacaggatat agcaggaacc
     3361 gggagacggc catgaacagg caggcgacag gtgtggcggg agccttgctg atgatgaccg
     3421 cgacgctggc gggaccggcg gccgcccagg atgcggagaa gatcgcgatg gcgcgggtcc
     3481 ggctcaccac cgagccgggc ctcacccagg gctgcacgcg catcggcccc gcgcgcgacg
     3541 attccatcaa ggacctgcgg cgcaagatcg tgaagatggg cgggaacacc gcactcatgg
     3601 ccttcggcgg caccgaggac ctctcgatgg tctacgccga ggtgttccgc tgcggcgatc
     3661 cgcccgccgc cgcgccgggc accgcgcccg cgcgcgtccc gctgcccccg gccgggccgc
     3721 cgccgcctcc accgccgccc cccgcaccca cgcgctgatc ggccggaccg acgcgcctgc
     3781 gccggccgtt ggggcgcttg ttcgggcacg ccacaagccc cgaagcgggc agaacgccag
     3841 atcggcggat gggcgccggc gcggaacctc ctgccgtctc gatgatccgg ccctggcaca
     3901 cgccttgcga tccgcaaacg gtcaggagct cgtccaagaa tacgcgacgc ctgggaggta
     3961 cccaccatgg gcgcgagagg acgaccgctg acccgctggt gtctggtcct ggccttgccg
     4021 gccatcgcct ggtttccgag catcgccgac agcgcggagc cggaggtcgg cgggatgatc
     4081 accgagatcc gcctgggcca gggacgggtc gaggtcggtt cggccggagg cgagcgccac
     4141 cgccccgcga ctcccctgct gacgctcagg gacggcgacg tcgtggccac cacgggtgac
     4201 gcctgggtca tcgtcgtcct gagcggcggg cgcggggcgg tcagggtgga cgaggccacc
     4261 tcgccgttcg tggtcacggc gccgccgccc gcgcggggcc agctccagaa ggggctgatg
     4321 atgctcgagg cgagcttcga cttcctctcg acgacatcga aggacgcgcc gttcggaaac
     4381 ctcggcagcc gggccggcgt gaggcctccg gtgatcctca cgccccgtaa cggccccgtc
     4441 cttcccgatt ccctcgtctt cgagtggcgg ggcagccaat cgtcgcgcta cgtggttcgc
     4501 atcgtcacgg ccgacgacgt ggtcatcgag cgcgccgatc tcgcggggac ccggttcgag
     4561 taccccaagg acgcgccccg gctggtggcc ggcgtccggt accggttcca gctcctcccg
     4621 gccgggcagc tgccccagga agtctggttc gaggtggtcg actcggcccg cgcccggacg
     4681 atccagcacg acctgcggga gatggacgcg gcgctccccg gcgggccgtc cgacaccacc
     4741 gcggccatcc tgcgggccgg gtttctggcc cagcatggtc tccttcacga tgcgcggctc
     4801 cgcgtggccg acgcgctcgc gcggcgaccc gacgagccca cgctttactt cctgctcggc
     4861 gagctctcgg cgcgtcaggg tctcccgaac gaagcgatgg aagccttcgc ggaggcgaga
     4921 ttcctcctga gcgctcccgc cacgccccgc taggcgccgg gcggagggcg ggacgggccc
     4981 ggtgctatca tgtcgcgtgg cctgggatcc cgcgcagtac ctgaagttcg ccgaccaccg
     5041 gctgcggccg gcgatcgatc tgctgaatcg catcgctctc gagcacccgg gcgacgtcta
     5101 cgacctgggc gccggcgcgg gcaacgtcac gcgcctcatc aaggagcgct ggcccgaggc
     5161 gcgcgtcacc ggcgtggacg actcgccggc catgctggcc aaggccgcgg ccaccgcccc
     5221 cgcgatcacc tggcagcagg cggacctggc cacctggagc ccgccgcggc cggccgatct
     5281 catctactcg aatgccgcgc tccactggct caccggccac gagcggctgt tcccggcgct
     5341 gctggccgcg ctcgcgcccg cgggcgtgct ggccgtccag atgccgcgca acttcggggc
     5401 gccgtcgcac acgctgatct cggaggccgc gcgcggcggg ccgtggcgcg cgaagctcga
     5461 gctcctgctc cggccggcgc cggtggccga tcccgatttc tactacgacg ccctcgcgcc
     5521 ccgcgcggcc gccctcgaca tctgggagac ggagtacctc cagatcctcg agggcgccga
     5581 tccggtcaag gagtggacca agggcacgtg gctcaagccg ctgctcgacg ccctcgagga
     5641 gccggagcgg agccgcttcg agacgcgcta cgccgagctg gtggcccgcg cctatccccc
     5701 gcgcgcggac ggccgcaccc tgttcccgtt ccggcggctc ttcatcgtcg cccgggcgcg
     5761 ctgaggacag gcatgaagat cgacgcgatc gacgtctact ggatgaagct cccgctcgcc
     5821 ttcgtgtgga agacgtccta cgccgaccag tacttcaccg acacgatcct ggtgcgcatg
     5881 gagggcggtg gccacgaggc ctggggcgag agctgcccgg tgttcatccc cgcctactcc
     5941 gccgagcaca ctctcgccac cttccacacc ctgcgcgagc acatggtgcc gcgcatcatc
     6001 gggcaggaca tcgagaccgc ccaggacctg ctcgaccggc tggccttcat caagggcaac
     6061 cagttcgcgc gggccgcgct cgacatcgcc tggtgggtgc tggacgccaa gcgacgcggc
     6121 ctgccgctgc accgcgccct cggcggcacc gccgaccggg tgacggtggg cgccgacttc
     6181 ggcatccagg actcgctcga catcctgatg gcaaagatcc agggggcgat cgacgcgggc
     6241 ttcccgcggg tcaagctcaa gttccggccc ggctgggatc tgttcatggt ggaggcggtg
     6301 cggtccacct tccccgactt caccttccac gtggactgca acgcggccta cacgcccgcc
     6361 gacaccgagc tcttccggaa gctcgaccga ttcgggctcg ccatgatcga gcagccgctg
     6421 gccgacgacg gcatgagcct ggtcaatcac gcggatctgc agcagcggat cgggacgccg
     6481 gtgtgcctcg acgagagcgc ccagagcctg gcgcacgtgc aggcggccat tcggctcaag
     6541 agctgccgcg tcgtgaacat caagatggcg cgggtgggcg ggctcaccga gtcgcgcgag
     6601 atccaggccc tgtgcgccca gcacgacatc ccctgctggg tgggcggcat gctggagagc
     6661 gcgatcggcg gggccatctg cgccgagctg gccaccctgc ccaacttcac ctatgcgggc
     6721 gatatcttcc cgtcctcgta cttctacgcg aacgatctcg gcaagcccga gatcgtgctg
     6781 agcgggcccg gggaggtcgc cacctcacgg gtgccgggca tcgcgcaggc gcccgacccc
     6841 gagctgctgg agcgctggac tgccgagcac gcgacgtttc gccccgcttc gacccggtaa
     6901 gaacgcccgg atttccccgc tcgcgtgccc gcgccgattg gtttgtcgcg atcttcgcgg
     6961 aaccaccctg gtggcatctc ggttgcttgc ggtagagagc atggtttctt catgcggctg
     7021 ccatcaagcc gtcgcaggtc ggggctactg tccagatcac ggagaccgat catgaacgtt
     7081 cgcgagaacg cggcgaagat cgcgacccag ttcgaaggcc gctgcacctg cgcggccatg
     7141 gacggcgggg tagcttgccc ctggtgccag gtcttccacg acgtgctcca gggccacccg
     7201 atcacgcctc cccccgcgcg tcggggcgac accgcgacgc cgaggctggc gcccacggcg
     7261 gcctgacagg ctcaggtcag gcgctggcgc ggctggcggc tggtctgcag gttgcgctgc
     7321 aggaacggga tgagcttgtc ccacgcgtcg cgcgcgggac cgtctcggta ggtggcgggg
     7381 ttggtgaagt tgaggaaggc gtgccccgcg tccgggtagc ggtggaactc gtgggccttg
     7441 cggtacttgt cgagctgggc ggagatccgg tccacgtccg ccggcgaggg attggcatcg
     7501 tccgcgccga agaagcccgc gatcggacag tgaatgtagg gcgtcagatc gaacggggtc
     7561 gggccgttcc cccaggcctt catgatgttg ccgccgtaga agacgcccgc cgccttgaac
     7621 accggcttgg aggccgccat caggtaggcg acgcgtcccc ccatgcagaa gccgatgatc
     7681 ccgacgtcgc cgagctgcgt gtccttcagc cgtctcgcgt agttgaccgc ggcattgacg
     7741 tccgcgatca cctccgggtc gcggagacgg ccgatgcggg tcatcatgtc ggcgtctgcc
     7801 gacacaccgg ccgcggcgct cgacgcatcg gccgcggcgc tggtctggcg gtggtagagg
     7861 tcgggggcga tggccaggta cccctgctgg gccaggttct ccacccggtc ctcgatgaac
     7921 ttgtccacgc cggggccgtg gtggatgacg atcaccgcgg gaaaggcccg gccgaggctc
     7981 ggcatgccga cgtagaggcg catcggggac ccatcgacgt ccaggcgatc ccactgcgcc
     8041 atgccgcgtc ttcctccgac tacgctcgtg agagagacgg cccgacggcg gaaccggtcg
     8101 tccgccgccg ggcctcagtt tctcgcgcgc aggctcatac ctacggcatg tcccgaccat
     8161 gggccgggat ctgggcgttg ctctagtcga gctcgcgcgt catcaggcag gactcacgcc
     8221 cggtgacgcg ccgtgcgagc cgggtggcgt gttctccata tcctgatctc cctgtgggtt
     8281 gaccgcacga cggtcggcgg cgcctcaccg tccgtcggca tcaagcaaac cgccgcgcgc
     8341 gctcgttgtc aagcggtcgg cggagtacac tgcggccatg cgccgttggc tcgccgcgct
     8401 cgctccgatc gccctgctcg tgctgggccc cgccggcgca tgggccgccg accggacaat
     8461 gggcagccag ggagctggcc agggaggcgg ccaaggaagc ggccatggaa gcggaatgac
     8521 tcggccgcac agccggccgc ccaggccaat tgtccggccg cactcagtca tcggctgctg
     8581 cctgggcgga gtgtcgttcc cgcccgccgc gccgccggtg gtcgtgagcc tgccaccccc
     8641 ggtctacgtc atcgtcccgt cgccgccgag cctggtcttc gtgccgacgc cgcgggtcga
     8701 gcccgcgccc gaggtcacgc tgccgaccgg ccgctgggca cgacacggca acggcgtgga
     8761 ctacccctac gtctgggtgt gggagggccc cgcgcggggg cgctgagccc cccgggccgg
     8821 ccgcgccgac tcaggcgcgg acgacgtagc cgttccggcg gttgctgcgg ccgatccctt
     8881 ggcgctcgga ctcgagcttg agctggccga gccggctcgg ccccgcccgc tccgcgtgcc
     8941 ggttgtagcg gtagcgccgc gcgaagccgt acttcttctg catgtcctca ggatttcgag
     9001 ccggcgcagc ccttcgatct gccattgcgg cgcgccggtc cacaccgcgt cggtccccca
     9061 gacggcgcgc ccgggcgccg gatggtttgc acgcggagac tctggcatac tcgagcgcga
     9121 tccggcgcga aaggagcggc tcccgatggc gcgagcgaat ctgaacggca tcgagatcga
     9181 ctacgaggcg accggggccg gccccgcggt gttcctgagc cacggctact ccgccacccg
     9241 ccgcatgtgg gacgatcagc accgcgcgct cggcgaccgc tatcgcgtca tcagctggag
     9301 catgcgcggg cacggcgcga ccgagagccc ggcggacccg gcgcggtact cggccccgct
     9361 caccatcgcg gacatgaagg ccctgctcgg ccacctcggg gtggagcggg cggtcatcgg
     9421 cgggctctcc ctcggcgggt acgtctcgct cggcttcgcg ctcgagtatc cgcagatgac
     9481 gcgcgcgctc gtgatctgcg actccggccc cggctaccgc agcgccgacg cgcgcgaggc
     9541 gtggaacgcg cgcgcccgcg agcgcgcggt gtcgctcgag acgcgcggcc tggacgcgct
     9601 cggccagagc cgcgaggtcc aggagtcggc gagcctccac cgctcggccc agggcctggc
     9661 ccacgccgca cggggcatgc tcgcgcagga gggctcgcgg gtgatcgatg ggctcgcggg
     9721 catccgggtg cccaccctca tcatcgtggg cgatcaggac cagccgtttc tcgccccctg
     9781 cgagtacatg gcgaagaaga tccccggggc gaggctcgag gtcatccggg gcgccggaca
     9841 ctcctcgaac ctcgaccagc cggaggcctt caaccgcgtg ctactccact tcctcgacgg
     9901 gctaccgccc gcctagacgc ccgttactgc acgggcgaga aggccgacgg gagcatgatc
     9961 ttgttggcca tcgtgagcag cgtgtcgcca ccgcccgggg gcggccacac ccccatctcc
    10021 cgcgccttgt tgcgcaccgc ggtccgctcg tcgaggctct tgtaggccca gatgtggatg
    10081 aagcggttgg cctcgccgtg ctcgacgtgc ccggccaggg ccaccggcga catcttgacg
    10141 cgctcctcga tcttcgactc ccagcgcttg atcaggtccg ggagcgtgcc gggcttcatc
    10201 gcgtagtagc gcatctcgaa gaacgggccc atcttgcccg gcttgaggtc gggcaggaac
    10261 gcgaacggca ccacgatctc cgactgcatg gtctcgatga actcggcgat cttgggcggc
    10321 cagttgcccg ccttcaccgc ctccgcccgg aggcggttgc gctcgcccag gtccgcgtac
    10381 ggccacacgt ggatgatctc gttcagcggg ccgatctcgg tgtgccagaa cgcggccagc
    10441 ggcgagatct tcttgcggtg ctcgtaggct tcgccgaagc gcttctccac ctcgggcagg
    10501 ctgcccacct tcacgcggta cgtccgaatc tcgtagatca cggctccact ccttttgtca
    10561 ctgcgtggga actgcaggcg ctgcggtcga gacgctactg ggccgtccat cctccgtcga
    10621 tcaccagctc gctcccggtc acgaacgacg actcgtccga ggcgaggtac agcacaccgt
    10681 aggccacctc gtccggctcg ccgtagcggc cgagcgggat gcgcgagacc atgtactcgt
    10741 agtgcccggg gtcgccgcgg cgcgcctccg tcatcggggt cacgatcgga ccggggtgca
    10801 ccgagttggc gcggatgcgc tcgcgcgcgt actgcaccgc ggtcagcttg gtcagcgtgc
    10861 gcaccgcgcc cttggatgcc tggtactgcg ggctgctgcg ctccaggccg acgatcccga
    10921 gctgcgagga gatgttgacg atcgagccgc cgccgctccg gcgcatcgcg gggatcgcag
    10981 ccttggtgcc gaggaagacg cccttggcgt tgacggccat gccgtgatcc cacgcctccg
    11041 cggtggtgtg ctcgaggagg ctgccgccgc ctacccctgc gttgttcacg agcacgtcga
    11101 gccgcccgaa gcgcccaacc gcggtggcca ccgcgcgctc ccagtccggc tcgctcgtca
    11161 cgtcgaggcg cacgtagact gcctccccgc ccttggccgc gatctccgcc tcgacccgct
    11221 tgccctccgc gtcgaggatg tcgcccagga ccacgcgggc gccttcccgc gcgaacagga
    11281 tcgcctccga cgcgcccatg ccgcgcgcgc ccccgctgat cagggccacc ttgccggcga
    11341 gtctcacttg gtgccgaaga gccggtcgcc cgcgtcgccc aggccgggga cgatgtagcc
    11401 gtgctcgttc aggtggctgt cgatggccgc ggtgtagacc ggcacgtcgg gatgcgcctg
    11461 gtggaagtgc tcgagccctt ccgggcaggt cagcaggcag acgaagcgga tcgagcgcgg
    11521 cgcggtggcc ttcacccggt tgaccgcggc gaccgccgag ttgccggtgg cgagcatggg
    11581 gtcgagcacg accacgtccc gctccgccat ctcgtgcggc accttgaagt agtactcgac
    11641 cgcctccagc gtcttggggt cgcggtagag gcccacgtgg cccacgcgcg cgctcggcag
    11701 gatgcggagc atgccgtcga ggaggccgtt gcccgcgcgc aggatcgaga tgagcacgat
    11761 cttcttgccg tcgagcagcc ggatggtggt ggggcccacc ggagtctccg ccgcgacctc
    11821 gtgggtgggc agctcgcggg tcacctcgta ggccagcagc atgctgatct cggcgagcag
    11881 gctgcggaac tcggacgtgc tcgtctcacg cttccgcatg aggccgagct tgtgctgcac
    11941 gagcggatgg ccgatgacga cgacgtcgtc cagggctcag agggctcgga gggggctgcg
    12001 ccccctgccg aacctccccc caagggcaga tcgcgcgggc caagcccgcg ctcggagcgg
    12061 accctccaac cggctacgcg tcatgatggg gtaccaggac agacgctaga ccagttcgag
    12121 ctgcgagcgg ccccaggtca tgtccacctc gcgggccggg ctgacctcgg ccggcccctc
    12181 gtcgggcgcg gtcggcccct cgccggccac ggtggtgcgg tgcatgaccc ggcggtactt
    12241 cgactcgtcc cacggccgcc cgcggtgaag catgcaccgg ttgtcccaca tgaccagatc
    12301 gccgacccgc cactcgtgcg tatagaccag ctcgggccgg gt
//
LOCUS       DASUFA010000275         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_871220, whole genome shotgun sequence.
ACCESSION   DASUFA010000275 DASUFA010000000
VERSION     DASUFA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871220"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFX87_10315"
     CDS             <1..424
                     /locus_tag="VFX87_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX4995355.1"
                     /translation="FFPIPGGRLKLRRGGDGAGELILYQRPDRPGPKESAYFRMDCPD
                     PGDLGAILGRRLGVRGVVEKRREVFLVGRARIHLDEVRDLGAYLEIEVVLADGEPAAQ
                     GEREARALLAALEMPDSALEARAYIDLLEARGLLRGLA"
     gene            436..966
                     /locus_tag="VFX87_10320"
     CDS             436..966
                     /locus_tag="VFX87_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4995356.1"
                     /translation="MTLETERLVLRMFGAADFEAYAAMCGDAEVMRFIGDGQPLARPM
                     AWRSLATVIGHWSLRGYGLWAATERASGALVGRVGFWNPDGWPGFELGWLLGRSYWGR
                     GYATEAARAALGWAFTQLGQPHVISLIYPDNAASIKVATRLGERLADRIELMGKPVLL
                     YRITRDEWQKGAAACR"
     gene            957..1697
                     /locus_tag="VFX87_10325"
     CDS             957..1697
                     /locus_tag="VFX87_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009999534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4995357.1"
                     /translation="MPVIFVHGVPDTDRVWRKVIARVARRDVVTVRLPGFGCPVPPGF
                     EATKDAYAAWLAEQIASQGEPVDLVGHDWGAILAVRAASLRPDCVRSWAAGGAPLDPD
                     YVWHPAAQAWQTPEVGEAVMQRLTPAALAKALAAAGVPPEDAGEAAGHLDETMKQCIL
                     RLYRSAVHVGREWLPELARIPRRGLVLWGEGDPYAGVEFGRRLAERTGARFAPLANCS
                     HWWQLERPDAVAAELAVLWGLAEHPGVR"
     gene            1710..2024
                     /locus_tag="VFX87_10330"
     CDS             1710..2024
                     /locus_tag="VFX87_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995358.1"
                     /translation="MKASRLLLGILLVYVFLDFANPFIDGAFCFDASDSLEALSQARA
                     RLAAPAAERSSGLPVPVLSLLAAPRGSTVVVRAASPRISRSPLIPVARAAIERGDGAD
                     PH"
     gene            2243..2755
                     /locus_tag="VFX87_10335"
     CDS             2243..2755
                     /locus_tag="VFX87_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4995359.1"
                     /translation="MRRSATTDRERRGASPRGRQRGLTLVELVIVVLIVGILSAVSIP
                     IYLGYTKEAKLAEGKAMAGSVLTALQGCTQAKGAGRSCTVTEITRRIGVSSTGRTADG
                     RWTVVRTGRLTVSSTTPPRFTGTIQIFGRTPNNVAVALSMFPTTTGVVLRCTTSSSTP
                     PASLTAGQPC"
BASE COUNT          354 a         1051 c         1052 g          444 t
ORIGIN      
        1 gttcttcccc atccccggcg gccgcctcaa gctccgccgt gggggcgacg gggccggcga
       61 gctcatcctc tatcagcgcc ccgatcggcc cggtccgaag gaatcggcgt acttccggat
      121 ggactgcccc gatcccggcg acctcggcgc catcctgggc cggcgtctcg gcgtgcgcgg
      181 cgtggtggag aagcggcgcg aggtgttcct ggtgggccgc gcgcgaattc atctcgacga
      241 ggtccgcgat ctgggcgctt atctcgagat cgaggtcgtg ctcgccgacg gcgagcccgc
      301 cgcgcagggg gagcgggagg cccgcgcgct cctcgccgcg ctggagatgc ccgactcggc
      361 gctggaggcg cgcgcctaca tcgatttgct cgaggcgcgg ggcctcttgc ggggcctcgc
      421 ttgacggcgg aggcggtgac gctcgagacg gagcgcctgg tgctccggat gttcggcgcg
      481 gccgacttcg aggcctatgc cgcgatgtgc ggggatgccg aggtgatgcg gttcatcggc
      541 gatggccagc cgctggcacg gcccatggcc tggcgcagtc tggccacggt catcggccac
      601 tggtccctgc gcggctacgg cctctgggcc gccaccgagc gcgcgtcggg ggccctggtg
      661 gggcgcgtcg ggttctggaa tcccgatggc tggcccggct tcgagctggg ctggctgctg
      721 ggccggtcct actgggggcg gggttacgcc accgaggcgg cccgcgccgc gctgggctgg
      781 gcgttcacgc agctcggcca gccgcacgtg atcagcctca tctacccgga caatgccgcg
      841 tcgatcaagg tggcgacgcg actgggggag cggctggccg atcgcatcga gctcatggga
      901 aagccggtgc tgctctaccg catcacgcgg gatgaatggc agaagggcgc ggccgcatgc
      961 cggtgatctt cgtgcacggg gtgccggaca ccgaccgggt gtggaggaag gtgatcgccc
     1021 gcgtcgcgcg ccgcgacgtc gtgacggtgc ggctgccggg cttcggctgt ccggtgccgc
     1081 ccggcttcga ggccaccaag gacgcctacg cggcctggct cgccgagcag atcgcgtccc
     1141 agggggagcc ggtggacctg gtggggcacg actggggcgc catcctcgcc gtccgcgccg
     1201 cgtcgctgcg gcccgactgc gtccggtcct gggcggccgg cggcgcgccc ctcgatcccg
     1261 actacgtgtg gcatccggcg gcgcaagcct ggcagacgcc cgaggtcggc gaggccgtca
     1321 tgcagcggct cacgcccgcg gcgctggcca aggcgctggc cgcggcaggc gtgccgcccg
     1381 aggacgcggg cgaggccgcc gggcacctcg acgagaccat gaagcagtgc attctccgcc
     1441 tctaccgctc ggcggtgcac gtcgggcgcg agtggctgcc cgagctggcg cgaatccccc
     1501 gccggggcct ggtgctgtgg ggcgagggag atccctacgc cggcgtggag ttcggccggc
     1561 ggctcgccga gcgcaccggc gcgcgcttcg cccccctcgc gaattgctcc cactggtggc
     1621 agctcgagcg ccccgatgcc gtggcggcgg agctcgccgt gctgtggggg ttggcggagc
     1681 acccgggcgt acggtagact cgcggcggga tgaaagcgtc ccgtctcctc ctcggcatcc
     1741 tgctcgtcta cgtcttcctc gatttcgcca atccgttcat cgacggggcc ttttgcttcg
     1801 acgcgagtga ttcgctcgag gcgctgagcc aggcccgcgc ccggctcgcg gcgccggccg
     1861 ccgagcgctc gagcggactg cccgtcccgg tgctgtccct tctcgccgct ccgcgcgggt
     1921 ccacggtggt ggtccgcgcg gcgtccccgc gaatctcgcg gagcccgttg atccccgtcg
     1981 cgcgcgcggc catcgagcgc ggcgacggcg ccgatccaca ctagccctcc tcctcccctc
     2041 gcatcgcacc gcggctcgct cccctccggg agccgtccat ctctcaacgg gaggaatttc
     2101 atgcgtcagg gctatgaagg aatcgtcgtc gcggggacca tgctgctcat cacgctcgtg
     2161 ctcgtgctcg ggatcgggta cctcttcgga tagccgtccc agaactttgt gaggccgggc
     2221 ccgtcgcata gactcgggag caatgcgacg ctcggcgacc accgatcgcg agcgacgggg
     2281 ggcatcgccc cgggggcgcc agcgcgggct caccctcgtg gagctggtga tcgtcgtgct
     2341 gatcgtcggc atcctctccg ccgtgtccat tcccatctat ctcggctata cgaaggaggc
     2401 caagctcgcg gagggcaagg ccatggcggg gtcggtgctg acggcgctgc agggctgcac
     2461 ccaggccaag ggcgccggcc ggagctgcac cgtgaccgag atcacccgcc gcatcggcgt
     2521 cagctccacc ggccgcaccg cggacggacg gtggacggtg gtgaggacgg ggcggctcac
     2581 cgtctcttcc accaccccgc cccgcttcac cggcaccatc cagatcttcg gacgcacgcc
     2641 gaacaatgtc gcggtcgcgc tcagcatgtt cccgaccacc accggcgtcg tcctccgctg
     2701 cacgacgtcc agcagcacgc cgcccgcctc gctgaccgcc ggccagccct gctagtcggc
     2761 cgcgcgccct gcgcccgggc cgagggggcg cttccggcgc tgtgatatcg taagttccca
     2821 tgccggcgcc cgccgcaatc cgcgacacca tactcgggcc gctcaatgac gctcagcgcg
     2881 aggcggtcac cgccatcgcg g
//
LOCUS       DASUFA010000276         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_991568, whole genome shotgun sequence.
ACCESSION   DASUFA010000276 DASUFA010000000
VERSION     DASUFA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991568"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..715
                     /gene="lexA"
                     /locus_tag="VFX87_10340"
     CDS             83..715
                     /gene="lexA"
                     /locus_tag="VFX87_10340"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX4995360.1"
                     /translation="MTARQREVLEFIRIFGERHGVPPTVREIGERFHFTARAAFDHLR
                     ALERKGMLERRVTGKRASRTLVLPAHKGARRSDPAAIPPGIPLLGRIAAGAPITAVEN
                     HEDTIPLRPDWLGMGGQDVFALRVRGDSMIEAHIMDGDLVLVRKQETAGSGDIVAAML
                     DGEATVKRFTRESGAVVLRPEHPTMKPIVVEAGRGDFRILGKVVGIMRQL"
     gene            730..990
                     /locus_tag="VFX87_10345"
     CDS             730..990
                     /locus_tag="VFX87_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995361.1"
                     /translation="MRRAGVAIRSRKRRPITRRDWKCDWRRDVRRRVWIGSRARVAAA
                     RPLFFTLNAQQVARVRAADLLGAAAMLLGAASWGMLAALLGS"
     gene            complement(1005..2903)
                     /locus_tag="VFX87_10350"
     CDS             complement(1005..2903)
                     /locus_tag="VFX87_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4995362.1"
                     /translation="MPRRVVWYPAGSPAATQLSALPGDLEALPLPAAGAPPAGPDESA
                     VVLVDLTAGDAAVARALAAAAPDLPVVALVAAGATPAVPCYAYVGKPAAPAVLAATLR
                     NACDHAHARREAAASRRELQELNQIGVGLSAERDTDALLDLILTKAREITHSDAGSLY
                     VVEDAADSTARLRFKLAQNDSVRVPFTEFTLPIDDASVAGHVALTGAVLQLDDAYLPP
                     PGSRFRVNRDFDERVGYRTKSMLVVPMTTPQGKVIGALQLINCKRDPAAVFPSPADVL
                     AHAIPYPPRFRDLAASLASQAAVALENSRLYQSIQTLFEGFVQASVTAIESRDPTTSG
                     HSFRVADFTVALATAVDRADHGAFREVRFSPDEMKEIRYASLLHDFGKVGVREEVLVK
                     AKKLPVGQLEIIRQRGEIIRRGLELRHARRQTDWLLQHGREGYGDRLAQWDAELAAVL
                     ADLDQHLKVVAAANEPTVMPDDVSASIRDLAGHAYADHLGDSLALLTPDEARTLALPR
                     GSLTPEEYEQIQSHVVHTYQFLKHIPWTKELRRVPEIARSHHEKLNGGGYPDGWRAPD
                     IPVQTRMMTISDIFDALTSRDRPYKPAVPVERALDILGHERTSGALDGDLLQIFIAVR
                     PWERAARV"
     gene            3015..3899
                     /locus_tag="VFX87_10355"
     CDS             3015..3899
                     /locus_tag="VFX87_10355"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX4995363.1"
                     /translation="MTTRVSTGSSRLDEMLGGGLLPGTLAVVYGATGIGKTHLGLGFC
                     DHGARADGARGIVFDMNARGDSQQHHAYAARLHGWDLKRWTHSVLPMAEPYPPEEQMQ
                     AFYCDALPWVGKLRDYQVASPDGVEFDWNWKAQYNQALYTVRPFVYFHLAAGSRRIVV
                     DGIEPMDVPGDYIQPYIFDDLYRKTIHRDSETLGMEICMPVWKHRAFIDAHRYDHAAV
                     TTLLLVTTEETQLEHLIARKVAAGDVGAVANTIVVMGSERVGNRLARMLCVVKHRGSA
                     KSEEIAEYRVTERGFELG"
BASE COUNT          592 a         1343 c         1532 g          577 t
ORIGIN      
        1 tagctcaacg ggtgttgact atcatcctgt tgaggcttgg ccagccaggg ccagacagga
       61 gaggggcgac caggtgaaag agatgacggc tcggcagcgc gaggtgctgg agttcatccg
      121 gatcttcggg gagcggcacg gagtgccgcc caccgtcagg gagatcgggg agcgcttcca
      181 tttcacggcg cgcgcggcgt tcgatcacct gcgggcgctg gagcgcaagg gcatgctgga
      241 gcgccgggtg accggcaagc gtgcctcccg gaccctggtg ctccccgcgc acaagggcgc
      301 gcggcggtcg gatccggccg ccattccccc cgggatccca ttgctcggcc gcatcgcggc
      361 gggcgcgccg atcaccgcgg tggagaacca cgaggacacc atcccgctcc ggccggactg
      421 gctcggcatg ggcggccagg acgtcttcgc gctgcgcgtg cggggggaca gcatgatcga
      481 ggcgcacatc atggacggtg acctcgtcct cgtgcgcaag caggagacgg cgggcagcgg
      541 ggacatcgtc gcggccatgc tggacggcga ggcgaccgtc aagcgtttca cccgggagag
      601 cggcgcggtg gtgctgcgcc cggagcatcc gaccatgaag ccgatcgtgg tggaggcagg
      661 acgcggcgac ttccgcatcc tgggcaaggt cgtcggcatc atgcggcaac tgtgaaagga
      721 gtggggacca tgcggcgggc aggagtagcg atacgcagca ggaagcggcg gccgatcacg
      781 cgccgcgact ggaagtgcga ctggcgtcgc gacgtgcggc gccgcgtgtg gatcggctcg
      841 cgcgcccggg tggccgcggc gcgccccctt tttttcacgc tgaatgctca acaggtggcg
      901 agagttcggg cggccgatct gctcggcgcg gccgcgatgc tactgggcgc ggcgtcctgg
      961 ggcatgctgg ccgcgctgct ggggagctga gcgccgggca ggattcagac gcgggccgcc
     1021 cgctcccagg gtcggaccgc gatgaagatc tggagcagat cgccgtccag cgcgccggac
     1081 gtgcgctcgt ggccgaggat gtcgagggcg cgctccaccg gcaccgccgg cttgtacggg
     1141 cggtcgcgcg aggtcagcgc gtcgaagatg tcggagatgg tcatcatgcg cgtctgcacc
     1201 gggatgtccg gggcgcgcca gccgtccgga tagccgccgc cgttcagctt ctcgtggtgc
     1261 gagcgtgcga tctcggggac gcggcggagc tccttcgtcc acgggatgtg cttgaggaac
     1321 tggtaggtgt gcacgacgtg ggactggatc tgctcgtact cctcgggtgt caggctcccg
     1381 cgcgggaggg ccagggtccg ggcctcgtcc ggcgtgagca gggccagcga gtcgccgagg
     1441 tgatccgcgt aggcgtgccc ggccaggtcg cggatcgagg cggagacgtc gtcgggcatc
     1501 acggtgggct cgttggcggc cgcgacgacc ttgagatgct ggtcgaggtc ggcgagcacc
     1561 gcggccagct cggcgtccca ctgagctaag cgatcgccgt agccctcgcg gccgtgctgg
     1621 agcagccagt ccgtctggcg gcgcgcgtgg cgcagctcga ggccccgccg gatgatctcg
     1681 ccgcgctgcc ggatgatctc gagctggccc accgggagct tcttggcctt gaccagcact
     1741 tcctcgcgca cgccgacctt gccgaagtcg tggagcagcg aggcgtagcg gatctccttc
     1801 atctcgtcgg gcgagaagcg gacctcgcgg aacgccccgt ggtccgcgcg gtccaccgcg
     1861 gtggccaggg ccaccgtgaa gtccgccacc cggaaggagt ggccggaggt ggtcgggtcc
     1921 cgcgactcga tcgcggtgac cgatgcctgc acgaagccct cgaacagcgt ctggatggac
     1981 tggtagaggc ggctgttctc gagcgcgacc gcggcctgcg aggcgagcga cgcagccaga
     2041 tcgcggaagc gcggcgggta ggggatcgcg tgcgccagca cgtcggcggg gctcgggaag
     2101 acggcggccg gatcccgctt gcagttgatg agctggagcg cgccgatcac cttgccctgc
     2161 ggcgtggtca tcgggacgac cagcatggac ttggtgcggt agccgacccg ctcgtcgaag
     2221 tcgcggttga cgcggaagcg cgagcccggc ggcggcagat acgcgtcgtc gagctgcagc
     2281 accgctccgg tgagcgccac gtggcccgcc acgctggcat cgtcgatcgg cagggtgaac
     2341 tcggtgaacg gcacccggac cgagtcgttc tgggccagct tgaagcgcag ccgcgcggtg
     2401 ctgtcggccg cgtcctcgac cacgtagagc gaccccgcgt cgctgtgggt gatctcgcgc
     2461 gccttggtca ggatcaggtc gaggagcgcg tcggtgtcgc gctccgcgga gaggccgacg
     2521 ccgatctgat ttagctcctg cagctcgcgg cggctcgcgg cggcctcgcg gcgggcgtgg
     2581 gcgtggtcgc acgcattgcg gagggtggcg gccagcactg cgggcgcggc cggcttgccg
     2641 acgtaggcat agcacggcac cgccggggtg gcccccgcgg caaccagggc gactaccggg
     2701 aggtcgggcg cggcggcggc cagggccctc gcgaccgccg cgtcgcccgc ggtcaggtcc
     2761 accagaacga ccgcggactc gtccggcccg gccggcggag cacccgccgc gggcagcggg
     2821 agcgcctcga ggtcaccggg gagcgcggag agctgggtag ctgccgggga acccgccggg
     2881 taccagacca ctcggcgtgg catgtatcga gggtaggacc cccctcggcg gcctgtcaag
     2941 cgagggcgac ctgccgggtc agacgccggc acggcttgac cgtctcccgc ccgggtcggt
     3001 aagatcaccc gcctatgacc acgcgcgtat ccacgggcag ttccaggctg gacgagatgc
     3061 tgggcggcgg cctgctgccg ggcaccctgg cggtcgtgta cggggccacc ggcatcggca
     3121 agacgcacct gggcctgggg ttctgcgacc acggcgcccg ggccgacggg gcccgcggca
     3181 tcgtcttcga catgaacgcc cggggcgact cccagcagca ccacgcctac gccgcgcgcc
     3241 tgcacggctg ggatctcaag cgctggacgc actcggtgct gcccatggcc gagccctacc
     3301 cgcccgagga gcagatgcag gccttctact gcgacgcgct gccgtgggtc ggcaagctgc
     3361 gcgactacca ggtcgcgagc ccggatgggg tcgagttcga ctggaactgg aaggcccagt
     3421 acaaccaggc cctctacacg gtacggccct tcgtgtactt ccacctcgcg gcgggcagcc
     3481 ggcgcatcgt ggtggacggc atcgagccga tggacgtgcc gggcgactac atccaaccct
     3541 acatcttcga cgacctctac cggaagacca tccatcgcga cagcgagacg ctcgggatgg
     3601 agatctgcat gccggtgtgg aagcaccggg cgttcatcga cgcgcatcgg tacgatcacg
     3661 ccgcggtgac cacgctgctc ctggtgacta ccgaggagac ccagctcgag cacctcatcg
     3721 cccgcaaggt cgcggcgggc gacgtgggcg cggtagccaa cacgatcgtc gtgatgggca
     3781 gcgagcgcgt cggcaaccgg ctggcccgca tgctctgcgt ggtcaagcac cggggcagcg
     3841 ccaagagcga ggagatcgcg gagtaccgcg tcaccgagcg gggcttcgag ctgggctgag
     3901 aggcatggac caggccgcgc tgctccggcg ccggcgtcgc aacttccgtc agcttccctc
     3961 gcaccgggtg cgctccgccc ggagcgcgct ggccttcgtg gaggcggtcg gcctctgctc
     4021 gaccttccat cgcttcccgg aagg
//
LOCUS       DASUFA010000277        23034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1443104, whole genome shotgun sequence.
ACCESSION   DASUFA010000277 DASUFA010000000
VERSION     DASUFA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 23034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23034
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443104"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFX87_10360"
     CDS             complement(<1..376)
                     /locus_tag="VFX87_10360"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995364.1"
                     /translation="MIAWLVLLQASLTIAVAGPPTSPEYLPLHVAQAEGLFATRQISV
                     TLRSMRSDATAAEMLATGQAQFAATSLDTALRLGGTEGVPPRLVWALTAAPPVALLVA
                     PGRAETIRSVADLVGQTVAIPAP"
     gene            complement(373..1491)
                     /gene="murG"
                     /locus_tag="VFX87_10365"
     CDS             complement(373..1491)
                     /gene="murG"
                     /locus_tag="VFX87_10365"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX4995365.1"
                     /translation="MRIVIAGGGTGGHTSPGLAVAARLRESGVDVHWIGSRAGIEAKR
                     VPEAGITFHTIPVGKLRRYWDWQNVPDLAVRAPAGLAQSWRLLRRLRPALLLATGGFV
                     ALPPALAARTLGIPLVVHEQTSVPGLANRVAGRFARRIALTFPLTGTEFPKERTVLTG
                     NPLRQELRGGTPAETHRRFNLDDSLPIVYVTGGALGAHRINRDVGEALPRLLEVCQII
                     HQCGDNAKTGDRAWLAERAQALPSLLRRRYALTAYVGAELRDVYAAAALVVGRSGAGT
                     VNECCQLGLAALYVPLPGTSGDEQTANARLVEAAGGAVVFPQVSLTPDGLVEAVTRLL
                     ADRTVLAAMGERARSLAVPDAADRIARLLFEVAAPTAP"
     gene            complement(1655..2773)
                     /locus_tag="VFX87_10370"
     CDS             complement(1655..2773)
                     /locus_tag="VFX87_10370"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX4995366.1"
                     /translation="MADKPPRLRVGVVFGGRSGEHEVSLAGAASVMAAMDKNRFELVP
                     IGIAKDGRWLMGGDPLRALAREAAQLALASGDPEAHTKHELLERASPQDTGTSLMRME
                     TSEGLPPGLRDRLDVVFVLLHGPQGEDGTVQGLLQLAGLPYTGAGVLASAVGMDKVAM
                     KDLFRAHGLPIVEYAVVRRHDWEREPAVIARGIAAEIGFPCFVKPANLGSSVGISKVK
                     AAEDLPAAIDLAASHDRRIIVERAIQAREVEVAVLGNDEPRVSVPGEVCFPGEWYDYD
                     TKYGAGQTTFKVPAPLSPEVTEEIRSLALRAFRATDCAGMARVDFFVEGDRRVLVNEI
                     NTIPGFTATSAYPRLWEASGIPYPELIARLIDLALERR"
     gene            complement(2787..4016)
                     /locus_tag="VFX87_10375"
     CDS             complement(2787..4016)
                     /locus_tag="VFX87_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995367.1"
                     /translation="MSDRAWLLAICLSELGTMLVFSSFSALLPLLKVEWGLSNTEAGL
                     ILAFYQVGYIGVVAVLATLTDYMPPRRIYLASAAWTALTSAAFAWWAGGFVSALVLRT
                     LVGLGLAGTYMPGMRMVAERFAHGRRGFAMGCYIGTFTLGTSISLLITGLANALWGWR
                     AAFAVTALGPAAAVLVGYGALSTETPRPAATASPARGGGFGPVLRNPSALRLITAYVA
                     HTFELMGMRLWIAPFFTASLMAAGAGLAEATQEGAYAASAVLAIGALPHPFSGLIVDR
                     FGRARPISTVMLLSAVCSLTIGWGLGWPFPALVALGLLYGILVTAESAMVSTGIADVA
                     EPAYLGRTMALQSSAGFAAGALGPAVFGAMLDWAPALGASPDSRWLWAFGVLGVVALA
                     GPLAVGFKEFSPRKTVV"
     gene            complement(4013..5272)
                     /locus_tag="VFX87_10380"
     CDS             complement(4013..5272)
                     /locus_tag="VFX87_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX4995368.1"
                     /translation="MRPRSLDEVVGQPQLTAPGKPLRRAVEEGQLHSMILWGPPGTGK
                     TTLARLLADVAGARFVPFSAVVSGVKEIRQVMTEAEAEGGRRGQRTILFVDEIHRFNR
                     AQQDAFLPYVEKGTIVLIGATTENPSFEVNGALLSRCRVYVLRPLGEADLVAILERAL
                     GDPERGLGGARATAEPEALALIARLSDGDARTALNVLEIAASDPGARVTEAAVRDAVQ
                     KKTLLYDKSGEQHYNLISALHKSLRDSDPDGALYWLGRMLEAGEDPLYVARRLVRFAS
                     EDVGNADPAALRLTLDAKEAYHFLGTPEGELALAQATCYLALAPKSNAVYAAWNAVRH
                     DIQEKPAEPVPLHLRNAPTGLMSNLGYGRGYQYAHDAPDAQVSQEHLPDALRGRVYYR
                     PTERGAEKEIGERLARWRAWREKQKRA"
     gene            complement(5409..6044)
                     /locus_tag="VFX87_10385"
     CDS             complement(5409..6044)
                     /locus_tag="VFX87_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4995369.1"
                     /translation="MGTGPAVCVFDLDHTLVNSPLDLRAVGRDMETFMRARGIRVPER
                     ALRWSGAELFDLVRREAAHLEAELLAIPVAHERRAMAAATLVPFAREAVAAMKDLGFA
                     TAIWTNNDRVVADFVLARFDLLPHLDLIVTRDEMHRLKPDPDGLRVVRARWPDAEQIV
                     VVGDSWVDGAAALAGGVPFIAYRPDRAEMEQRGVVIAAEIHSLLDLPAALA"
     gene            complement(6151..7152)
                     /locus_tag="VFX87_10390"
     CDS             complement(6151..7152)
                     /locus_tag="VFX87_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4995370.1"
                     /translation="MAREASQIPPPSGLRVNRKSAVPVHVQLQTQIRHLINIGSLKPG
                     MQLPTVRQLAGFLRINRNTVARALADLDQDGYLESHQGRGTFVADRPPAHEGRAARSL
                     ERLVQETLERTRRLGFTHEELTATIAGQAPASRSAPRAKVRTLLVECNHPELTRYREQ
                     LEEELPIVVDRLLVEELDARLREPGFLAGYRVVITTFFHIHEVKQALPLDGPPAVALL
                     SESNIASLLRLTELAEGTAVGLICNTPRGSQNLLSSVQSAGLSHLVPILAAADDPWSI
                     DRLLEKTRIVVCSEQAAGRIRERVPHDVEVILADRRLDTGGVDLLRDVLAQLDGERA"
     gene            complement(7167..7373)
                     /locus_tag="VFX87_10395"
     CDS             complement(7167..7373)
                     /locus_tag="VFX87_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995371.1"
                     /translation="MNTHLYEQLVRERLDEARATAAQLALLRKLGPARRPVRVAIGFA
                     LIKVGHWVAGRAPKLAAGPRRAAA"
     gene            complement(7509..8228)
                     /locus_tag="VFX87_10400"
     CDS             complement(7509..8228)
                     /locus_tag="VFX87_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995372.1"
                     /translation="MAEPVASAPAPVFPPVIVLIEDEPQIRRFLRATLGTQGYRLYEA
                     TSGEDGLIEAAARQPDVVILDLGLPDLDGLAVIRRLREWSRVPIIVLSARGQERDKVA
                     ALDAGADDYVSKPFAVGELLARLRVALRHVAGGESGEPTFTVGDLRVDQVKRQVQLDG
                     KDVHLTPIEYRLLTTLVKHAGRVLSQRHLLKEVWGPAHTDQAHYLRVYMGALRRKLER
                     DPARPRYLLTEPGVGYRLAAE"
     gene            complement(8221..10914)
                     /locus_tag="VFX87_10405"
     CDS             complement(8221..10914)
                     /locus_tag="VFX87_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEX4995373.1"
                     /translation="MTTERRPDPDELLARVKAAETRQARGRLKIFFGAAAGVGKTYAM
                     LEAAREQRAQGMDVVVGWVETHGRAETEALLGNLEILPRRLVEYRGTTLREFDLDAAL
                     ARRPALMLVDELAHTNAPGSRHAKRWQDVVELLDAGINVYTAMNVQHVESLNDVVAKI
                     TGVVVRETVPDSIFEHTDEVELIDLPPDDLLHRLRDGKIYIPDQAREAVENFFRKGNL
                     IALRELALRVTAQRVDAEMRDYMRDHAIHQTWPVRERLLVCIGPSPSSIRLVRAAKRM
                     AEGLGAEWIVAYVETAAQTRLTQDARDRVAQTMRLAEQLGAETHTLTGARMSDEILAF
                     ARARNVSKIVVGKPERSLWKRIAMGSIVDTLVQGSGEIDVYVISGDRDDSRPMPPRTW
                     QRQTDWAPYVAAVVSVAGSTAIAWAMFPYFAVSNLIMVYLLSVIMVATRYGRGGSLLA
                     TLLSVAAFDFFFVPPYFTFAVSDTQYLVTFAVMLVVAGVISSLAVRIRAQAESARERE
                     RRMAALYAMSRELAGTRGVHQLREVAARHIAEVFRTRVAVLLPQPDGRLAPDEGDAAQ
                     FPMDANELAVGQWVHEHGQVAGQGTDTLPGASALYVPLTGSRGTVGVLGLRPQDPGPL
                     QAPEQLHQLETFASQTALAIERARLAEEAERAQVRAETERLRNSLLSSVSHDLRTPLA
                     SITGAASTLLENEARLDASTRRDLLESLHEEADRLNRLVQNLLEMTRLESGALQLHTE
                     WHPVEEVVGAALGRFGKALARRAVTTRVPPELPLVPMDDVLIEQVLINLLDNVLKYTP
                     AGSPIEVSAEDGGGAVLIEVADRGPGLRPGEERRIFEKFHRAEAAPSQRGAGLGLAIC
                     QGIVNAHGGHIWAENRPGGGVRVRFTLPVKEAPPILSEHDG"
     gene            11171..11260
                     /gene="kdpF"
                     /locus_tag="VFX87_10410"
     CDS             11171..11260
                     /gene="kdpF"
                     /locus_tag="VFX87_10410"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX4995374.1"
                     /translation="MVLYVAGALVSAGLLVYLVVALLKPELFQ"
     gene            11257..13062
                     /gene="kdpA"
                     /locus_tag="VFX87_10415"
     CDS             11257..13062
                     /gene="kdpA"
                     /locus_tag="VFX87_10415"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX4995375.1"
                     /translation="MTVNALFQLVLYIVVLLALARPLGAYMARVYEGRRLALDRVLGG
                     LERFVYWAAGVWPNAEMGWKSYALAMLWFNLLGVLAVYLLQRLQGVLPLNPQGLAAVS
                     PDSAFNTAVSFASNTNWQGYGGETTMSYLTQMLALGVQNFVSAASGMATLVALIRGFA
                     RRSADTIGNFWVDLTRGTLYILLPLSFVLALVLVSQGVVQTLNPSVTATVVQPSQYEV
                     PETGADGKPALDATGRPRMKTSTLTEQVIAVGPAASQIAIKQLGTNGGGFFNVNSSHP
                     FENPTALANFLEVLAILVISAALCYTFGVMVKDTRQGRTVLAAMSVIFVVLLAVCYVA
                     EQNGAVFVKQGVDHTAGALQSGGNMEGKEVRFGIANSALWATATTAASNGSVNSMHDS
                     YTPVGGLVPMWLIQLGEVVYGGVGSGLYGMLVFAIIAVFVAGLMVGRTPEYLGKKIEA
                     YEMKMAALVILIPPAMVLIGTAVAMVTPAGKDGILNPGAHGLSEVLYAFSSAGNNNGS
                     AFAGLSANTPFYNTALGFAMLFGRYWLAIPTLAIAGSLARKKTVPVGPGTLPTDTPLF
                     AGLLIGVVILVGALTFLPALALGPIVEHLIVIVAK"
     gene            13083..15146
                     /gene="kdpB"
                     /locus_tag="VFX87_10420"
     CDS             13083..15146
                     /gene="kdpB"
                     /locus_tag="VFX87_10420"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX4995376.1"
                     /translation="MVRARSLFDAAIVKQAIVDSVRKLTVVRQVRNPVMFVVYVGSIL
                     TTLLWVQALVGKGEAPSWFIFLVSVWLWFTVLFANFAEAMAEGRGKAQAASLRNARRD
                     LQAKRLPRPERTTRYEAVSASALRRNDLVLVEAGDFIPADGTVVVGIASVNEAAITGE
                     SAPVIREAGGDRSSVTGGTQVLSDWIIVEVTANPGEAFLDRMIALVEGAKRQKTPNEI
                     ALDILLAALTIVFLLATATLLPFSLYSVAAAGQGAPVTVTVLVALLVCLIPTTIGALL
                     SAIGIAGMDRMIQKNVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQATAFVPAAGVT
                     ETALADAAQLASLADETPEGRSIVVLAKEKYGIREREIAKLGATFVAFTAQTRMSGVN
                     LNSRQVRKGASDAIEAHVKHLGGTFPPEVRQSVDTIAKAGATPLVVCDGARVLGAIHL
                     KDIVKGGIRERFAQLRRMGIKTIMITGDNPLTAAAISAEAGVDDFLAQATPEAKLKLI
                     RDVQAQGRLVAMTGDGTNDAPALAQADVALTMNTGTQAAKEAGNMIDLDSNPTKLLEV
                     VETGKQMLMTRGALTTFSIANDVAKYFAIIPAAFATTYPALGALNIMGLATPESAILS
                     AVIFNALIIIALIPLALKGVRYRALGAAAVLRRNLWMYGVGGLVIPFPGIKIIDMILV
                     ALKLV"
     gene            15161..15727
                     /gene="kdpC"
                     /locus_tag="VFX87_10425"
     CDS             15161..15727
                     /gene="kdpC"
                     /locus_tag="VFX87_10425"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX4995377.1"
                     /translation="MAHLRPALMSLLLLTVITGVAYPLLVTGVAQAVFPSQAGGSLIV
                     RDGKVVGSALIGQPFDDPKYFWGRPSATSPFAYNAGASSGSNLSPTNPALIAAVQERV
                     GALRAADPGNTAPVPVDLVTASGSGLDPDISPAAALYQVPRVARVRGLAPDAVRALVE
                     RHTERRFLGVLGEPRVNVLALNLAVDGR"
     gene            15744..16496
                     /locus_tag="VFX87_10430"
     CDS             15744..16496
                     /locus_tag="VFX87_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX4995378.1"
                     /translation="MNRADDARVWMVTGVLGSMALAVALIPLRTQVAASNLAFVFIAF
                     TIIVAELGGRGAALATALIAAMSLNFFLTEPYLTLAISKTDDLVAFVALAACGLIAAA
                     FGRRRERLSDVAGRADQELSTLARFAERSRSGRPPDALLQDLRTEFDLGGLVLRDGSG
                     RVLAAVPADAAARPAPRMALDGTTLFAAAEETPRIGANGLRLPEGGGRLTLQTARGPV
                     SLDLWEGNDQGFGLDESRTLAIAVSILGLGIR"
     gene            16532..17959
                     /locus_tag="VFX87_10435"
     CDS             16532..17959
                     /locus_tag="VFX87_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4995379.1"
                     /translation="MKPTSLRVFVTTLGVLVAWPSLALAVGEPERLWTVGDRAFQDGL
                     YGVSRRMLERLVERYPTDGRIPDATLLLGKVRLSQGQHDAALQAFRQAQTFSPPPGRP
                     EEARFWEGETLFRMKRFPEARAIYDRILAESPSSPSAPDAVYGLAWVNLEMKRRDQAV
                     TEFRRILSSYPEHANVSSATFYLARTLLELKRADEAVGLLRGFVARYTDHRLLPDARY
                     TLGQALIAGGQSDEGVAELRAFAKAYPNHELAGAARRNVVDTLVRQGKKNELAEEYKV
                     LASQPATAESLYDAGLVASKIGRPRDADTAWARLRQDFPDPPLSGRVSLEMAQSAFAK
                     NSFKDASTLARAASRSPEDAVRGEAFVLLGESELKQRRPAAAITAFKSAAEAPALDPA
                     MRFRALAGTGLAHEEQRQYGPAAKLYEEVAAKSPDKTLAAWAKERLAAVNAAARSEAP
                     PAEKATPKKAAPQKTAPKQAPGGTK"
     gene            17956..20946
                     /locus_tag="VFX87_10440"
     CDS             17956..20946
                     /locus_tag="VFX87_10440"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4995380.1"
                     /translation="MIRPRALLVAALLVLLGGATARAEIALMLPAPDVGGLVPLAAPA
                     LDKPPVPLPVVPLPPAPQGMPELPPAPMVGHLGERPVAPLPSPRFLACNPVGNLLGVA
                     SELIECGRARYQRNELEEGRAVLEKAVQFATERTQIREARYWLGETLLRLNKPAEVER
                     LMLQVQQDDPRGELGLYAAHQLGWVSLELNRPDRALTYFDTLLKVGAPPAIISYARHG
                     RGLALYGLKRYPEARDTWAGLLNQSAPRAVAWEGTFWLGDTLGRLGDHAGAVQRLQTF
                     VSGGPQVLIDSGLLRLGWWNRAAGQPLEAAKAYRGLLSAYPQSREAAWARAGLVQALL
                     DLDDYPAAREEAKKLEAMDRSGALAVPVRLLISRWATEKGRFDEAREINDELLARTLD
                     PATRSYVLALSAEASRASGQVGEARDRFELVRGTPGSPALAAYAALRLAQIDFDTREF
                     AKARDGARKLVADTAAPAEMRAAALVLGGEAAYWARDYTQAAAAYSGFLSAYPGYPQA
                     PMAGLALGWAELQGGKPEAARQRWTRYAQQAQSDPRAPEALVLAAELAGRAGDFAGAQ
                     TLLDQVVDRYPNSPYAEVAMLNRAILAVRGGRAADALPELGRLVQRGAVSPYVARARV
                     TRGAALLATGKPADAERDFQAAQSLGETGVAYLGMGTADFARQQWDAAVRDFVQARDA
                     GAGPVSAAASYGLAAVLFNQRRLDEFKQAATPIVAGAVDPIITPRLLHGLAAVATEEK
                     RWPEAKTLTLRLADQFPRYEGTQAALTTLATAAGQGEQWMLSREMYAKLGERFPGSAT
                     AGANRLDYAEALLRTGAAAEARRALEPAAGAATAPAPTPRATMLMAEAQEATGDRVAA
                     LDTYVRAAAAAGSPKEKALGLLGQGRLLVADGKWDEARGRLEQALDAGDGPVAAEAAY
                     RLGEGYRATGKAQDAADMYMTAAYLANSPWARRALVGAGQAFAALKQNDAAVIVYRKL
                     LAMSGVEPDLAEAARRGLRALGAS"
     gene            complement(20954..21373)
                     /locus_tag="VFX87_10445"
     CDS             complement(20954..21373)
                     /locus_tag="VFX87_10445"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX4995381.1"
                     /translation="MFCGQQVAGLSKRAAEIERRGGMLVVVGPAKPEHIAGFRERTGY
                     KGALFVDPALRAFKTAGLVRGRASTYHPLAVLKGVRAMAQGFRQVGRQGDVIQQGGTF
                     VLGPGDRVRYEWRDRYAGDHPHLDEVVEALPSGEGTG"
     gene            complement(21374..21502)
                     /locus_tag="VFX87_10450"
     CDS             complement(21374..21502)
                     /locus_tag="VFX87_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995382.1"
                     /translation="MDDLLAAALGQMSVLDETGAEVRLGSFWIKRPVVLAFVRHFG"
     gene            21547..>23034
                     /locus_tag="VFX87_10455"
     CDS             21547..>23034
                     /locus_tag="VFX87_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX4995383.1"
                     /translation="MRGYGGRILFVDVTDGRSRVESVSPELARSLLGGNGFAARLLLD
                     HVPAGVDAYDPANAVIFAVGPITDTTVPGNSRACVAGKSPLTGLFFDSTFGGRWPATM
                     KRTGFDAIVITGRAAEPVYVKVTEAGAELKPARGLWGKTTRDTVHAIQAVEGGDVDAM
                     AIGPGGENLVRFAAMGTYWKNREAFAGRGGLAAVLGSKRVKAVVVAGSRKTEVADAAA
                     LKSLLEQQREPLKTGTKALSTYGTPFLVKPINTLGALGSYNLRQEVFADAMAISGEEM
                     HAHFHDKDTTCLKCPVACGKQYEIREGELAGLKAKMPEYETIFAFGSMLGNGHAASLA
                     RANDLCDLLGLDTISMGVTLAFVCEALERGWLTESEIGLAFGWGDWRGMLKLVEMTAA
                     REGFGDRLAEGGWRLAESVHAEGTRLVYAVKRLELPAHSARALKGMSIGYATATRGGS
                     HHDTRPTPQYAQNYDRRSPEGKPEFAVRSQHFTAVDDSLVLCRFTS"
BASE COUNT         3253 a         8205 c         8362 g         3214 t
ORIGIN      
        1 cgggcgccgg aatggcgacg gtctgcccga cgagatcggc cacggagcgg atggtctcgg
       61 cgcggccggg cgcgacgagc agcgcgacgg gcggcgcggc ggtgagggcc cagacgagtc
      121 gcggtggcac gccctcggtc ccgccgagcc gcagggccgt gtcgagcgag gtcgcggcga
      181 actgggcttg cccggtcgcc agcatctcgg ccgcggtggc gtccgagcgc atggaccgga
      241 gcgtcacgga gatttgccgg gtggcgaaga ggccctcggc ctgcgccacg tgcaggggca
      301 ggtactcggg cgaggtgggc ggccccgcca ccgcgatcgt gagcgaggcc tgcagcagca
      361 ccagccaggc gatcatggcg cggtcggagc cgccacttcg aaaagcagcc gcgcgatgcg
      421 atcggccgcg tcgggcacgg cgaggctccg agcccgttca cccatcgccg cgaggaccgt
      481 ccggtccgcc aggaggcgcg tcacggcctc cacgaggccg tcaggcgtga gcgagacctg
      541 cgggaatacc acggcccctc ctgcggcctc cacgaggcgt gcgttggccg tctgctcgtc
      601 tccgctggtg cctggcagcg ggacgtagag ggcggccagg ccgagctggc agcactcgtt
      661 gaccgttccc gcgccgctgc gcccgacgac gagcgcggcc gccgcgtaga cgtcgcgcag
      721 ctcggccccg acgtaggcgg tcaaggcgta gcggcggcgg agcagcgaag gcagtgcctg
      781 ggcgcgctcg gcgagccagg cccgatcgcc cgtcttcgcg ttgtcgccgc actggtggat
      841 gatctggcag acctcgagca ggcgaggcag cgcttcgccg acgtcccggt tgatccggtg
      901 cgcgcccagc gcgccgcccg tgacgtacac gatcggcagc gagtcgtcca gattgaagcg
      961 gcggtgagtc tccgccggtg tccccccacg cagctcctgg cgcagcgggt tgccggtgag
     1021 cacggtgcgc tccttcggga actccgtgcc ggtgagcggg aacgtgagcg cgatgcggcg
     1081 cgcgaagcgg ccggccaccc ggttggcgag cccgggcacg ctggtctgct cgtggacaac
     1141 cagagggatg ccgagcgtgc gcgccgcgag ggccggcggc agcgcgacga agccgcccgt
     1201 ggcgaggagc agcgccggcc gcaggcgacg gagcagccgc caggactgcg ccaggccggc
     1261 gggcgcccgc accgccaggt cgggcacgtt ctgccagtcc cagtagcggc ggagcttgcc
     1321 caccgggatc gtgtggaagg tgatcccggc ctcgggcacg cgcttggcct cgatgccggc
     1381 ccggctgccg atccagtgca cgtccacgcc cgactcgcgg agccgagcgg ccaccgcaag
     1441 cccggggctc gtgtggccac ccgtgccgcc gcccgcaatg actatgcgca tgcggggact
     1501 tgccgcgggc aatcgccgcg ggcaatccgc tgggagcgga caatcagatc cgcccctctt
     1561 tctaatgcgg gggcccagaa atggcccccg cactccccca atccgcggac aaccgttccg
     1621 gccaggcccg gtcgcccgcc cacccggccg cccctcaccg gcgttcgagc gccaggtcga
     1681 tgaggcgggc gatcagctcc gggtagggga tgcccgaggc ttcccagagg cgcgggtagg
     1741 cgctggtcgc ggtgaagccc gggatcgtgt tgatctcgtt cacgagcacg cgccggtcgc
     1801 cctcgacgaa gaagtccacg cgcgccatgc ccgcgcagtc ggtggcgcgg aaggcgcgca
     1861 gggccagcga gcggatctcc tccgtgacct cgggcgacag cggcgccggc accttgaacg
     1921 tggtctgccc cgcgccgtac ttggtgtcgt agtcgtacca ctcgccgggg aagcagacct
     1981 cgccgggcac ggagacgcgc ggctcgtcgt tgccgagcac cgccacctcc acctcgcgcg
     2041 cctggatcgc gcgctcgacg atgatgcggc ggtcgtggct cgcggccaga tcgatggcgg
     2101 cgggcaggtc ctcggccgcc ttcaccttgc tgatcccgac gctcgagccc aggttcgccg
     2161 gcttgacgaa gcatgggaag ccgatctcgg ccgcgatgcc gcgcgcgatc accgcgggct
     2221 cgcgctccca gtcgtgacgg cgcaccaccg cgtactccac gatgggtagc ccgtgggcgc
     2281 ggaagagatc cttcatggcg accttgtcca tgccgactgc ggaggcgagt acgcccgcgc
     2341 cggtgtacgg caggccggcg agctgcagca ggccctgcac ggtgccgtcc tcgccctgcg
     2401 ggccgtggag cagcacgaag accacgtcga gccggtcgcg gaggccgggc ggcaaccctt
     2461 cggacgtctc catgcgcatg agcgacgtgc cggtgtcctg gggcgaggcc cgctcgagca
     2521 gttcgtgctt ggtgtgcgcc tcgggatcgc ccgaggccag ggcgagctgg gcggcctccc
     2581 gggccagggc gcgcagcggg tcgccgccca tgagccagcg gccgtccttg gcgatgccga
     2641 tgggcaccag ctcgaagcgg ttcttgtcca tcgccgccat cacggaggcc gcgccggcca
     2701 gggagacctc gtgctcgccc gagcggccgc cgaagaccac tcctacccgg agcctgggcg
     2761 gcttgtcagc catgatttca gttgtatcag accacggttt tccgggggga gaactccttg
     2821 aaccccaccg cgagcgggcc ggccagggcc accacgccga gcaccccgaa ggcccagagc
     2881 cagcgagaat cgggggaggc gccgagtgcg ggcgcccagt cgagcatggc gccgaagacg
     2941 gccgggccga gcgcgccggc cgcgaagccc gccgaagact gcagcgccat ggtgcggccg
     3001 agataggcgg gctcggcgac gtccgcgatg ccggtggaga ccatggccga ctccgcggtc
     3061 accaggatcc cgtagagcag cccgagcgcc acgagggcgg ggaacggcca gccgagtccc
     3121 cagccgatcg tgagcgagca gacggcggag agcagcatca cggtgctgat cgggcgagcc
     3181 cggccgaacc ggtccacgat gagcccggag aagggatgcg gcagggcgcc gatggccagc
     3241 accgccgagg ccgcgtacgc gccctcctgc gtggcctccg cgaggccggc gcccgccgcc
     3301 atcaggctcg cggtgaagaa cggcgcgatc cagaggcgca tgcccatcag ctcgaaggtg
     3361 tgggccacgt aggcggtgat gagccggagc gcgctcgggt tgcgaagcac cggcccgaag
     3421 ccgccgccgc gcgccgggct cgcggtggcg gccggccgcg gcgtctccgt ggacagcgcc
     3481 ccgtagccga cgagcacggc ggcggcgggt ccgagcgcgg tcaccgcgaa ggccgcgcgc
     3541 cagccccaga gcgcattggc gaggccggtg atcagcagcg agatcgaggt gccgagcgtg
     3601 aacgtgccga tgtagcagcc catcgcgaag ccgcgccggc cgtgcgcgaa ccgctcggcc
     3661 accatgcgca tgccgggcat gtaggtgccg gccaggccca ggccgaccag cgtgcgcagc
     3721 accagcgccg agacgaagcc cccggcccac caggcgaagg cggcgctggt gagcgcggtc
     3781 caagcggcgc tcgccaggta gatccgccgc ggcggcatgt agtcggtgag cgtggccagg
     3841 accgccacca cgccgatata ccccacctgg tagaaggcga ggatgaggcc ggcctcggtg
     3901 ttactgagcc cccactcgac cttgaggagc ggcaggagcg ccgagaagct cgagaagacg
     3961 agcatggtgc ccagctcgga gaggcagatg gccaggagcc aggcgcgatc gctcacgccc
     4021 gcttctgctt ctcccgccat gcccgccacc gggcgagccg ctcgccgatc tccttctcgg
     4081 ccccgcgctc ggtgggccga tagtagacgc ggccgcgcag ggcgtcgggc aggtgctcct
     4141 ggctcacctg cgcgtcgggc gcgtcgtgcg cgtactggta gccgcggccg tagcccaggt
     4201 tgctcatgag gccggtcggc gcgttccgca ggtgcagcgg caccggctcg gccggcttct
     4261 cctggatgtc gtggcggacc gcgttccagg ccgcgtagac cgcgttcgac ttcggcgcca
     4321 gcgccagata gcaggtggcc tgggcgaggg ccagctcgcc ttccggggtg ccgaggaagt
     4381 ggtaggcctc cttggcgtcg agggtcaggc gcagcgcggc cggatccgcg ttgcccacgt
     4441 cctccgaggc gaagcgcacg agccggcggg ccacgtagag cgggtcctcg cccgcctcca
     4501 gcatgcggcc gagccagtag agcgcgccgt ccggatcgga gtcgcgcagc gacttgtgga
     4561 gcgcggagat caggttgtag tgctgctcgc ccgacttgtc gtagagcagg gtcttcttct
     4621 gcaccgcgtc gcgcaccgcc gcctcggtca cgcgggcgcc cggatcgctc gccgcgatct
     4681 cgagcacgtt gagcgcggtg cgcgcgtcgc cgtcggagag ccgcgcgatg agggccagcg
     4741 cctcgggctc ggcggtggcg cgggcgccgc ccaggccgcg ctcggggtcg ccgagcgcgc
     4801 gctccaggat ggccacgagg tcggcctcgc cgagcgggcg gagcacgtag acccggcagc
     4861 gcgagaggag cgcgccgttg acctcgaacg acgggttctc ggtcgtcgcg ccgatcagca
     4921 cgatggtgcc cttctcgacg tagggcagga aggcgtcctg ctgcgctcgg ttgaagcggt
     4981 ggatctcgtc cacgaagagg atcgtgcgct gcccgcgccg gccgccctcg gcttccgcct
     5041 cggtcatcac ctggcggatc tccttcacgc ccgagaccac cgccgagaac ggcacgaagc
     5101 gggcgcccgc cacgtcggcc aggaggcgcg ccagcgtggt tttgccggtc ccgggcgggc
     5161 cccagaggat catcgagtgg agctggccct cctcgacggc ccggcgcagc ggcttgcccg
     5221 gcgcggtgag ctgcggctgg ccgacgacct cgtcgaggct cctcgggcgc atgcggtcgg
     5281 ccagcggcgc gggcgccttc ggggcggagg cgggcggcgt atcgaacagg tccacgcggc
     5341 gaattatacg cgcctcactc tgccacgctc tccccttgag gggagagggc agggtgaggg
     5401 ggtggtgctc aagcgagcgc ggcgggcaga tcgagcagcg agtgaatctc ggctgcgatc
     5461 acgaccccac gctgctccat ctcggcgcgg tccgggcggt aggcgatgaa gggcacgccg
     5521 ccggccaggg ccgccgcgcc gtccacccac gagtcgccca cgaccacgat ctgctccgcg
     5581 tcgggccagc gcgcccgcac cacgcgcaac ccgtcggggt cgggcttgag ccggtgcatc
     5641 tcgtcgcggg tgacgatcag atcgaggtgc ggaagcaggt cgaagcgggc gaggacgaag
     5701 tcggcgacca cccggtcgtt gttggtccag atagcggtgg cgaagccgag gtccttcatc
     5761 gcggccaccg cctcgcgcgc gaacggcacg agggtcgccg cggccatggc ccgccgctcg
     5821 tgtgccaccg ggatcgcgag cagctcggcc tcgaggtggg cagcctcgcg tcgcaccagg
     5881 tcgaacagct cggcgcccga ccagcggagc gcccgctcgg gcacgcggat gccgcgggcg
     5941 cgcatgaagg tttccatgtc gcggcccacg gcccgcagat cgagcggaga gttgaccagc
     6001 gtgtggtcga gatcgaagac gcagaccgcc ggcccggtac ctatgccacg gcctccggct
     6061 cgtcgagcac cagcgtgcgc aggacgagcc gctcgtcctc ggtcacgagg gcctcgtggg
     6121 cgccgcgggc cagcaggagg cgggccgtgt tcatgcccgc tcgccgtcga gctgggccag
     6181 cacatcccgc agcaggtcca cgccgcccgt gtcgagccgc cggtcggcca ggatcacctc
     6241 gacgtcgtga gggacccgct cgcggatgcg cccggccgcc tgctcggagc agaccacgat
     6301 gcgcgtcttc tcgagcaggc ggtcgatgga ccagggatcg tcggccgcgg ccaggatcgg
     6361 caccaggtgg gagagcccgg cggactgcac ggagctcagc aggttctggc tgccgcgcgg
     6421 ggtattgcag atcaggccga ccgcggtgcc ttccgcgagc tcggtcaggc gcagcaggga
     6481 ggcgatgttc gactcggaca gcagcgccac cgcgggcggc ccatcgagcg gcagggcctg
     6541 cttcacctcg tggatgtgga agaacgtggt gatcaccacg cggtagcccg cgaggaagcc
     6601 cggctcccgc aggcgcgcgt ccagctcctc cacgagcagc cggtccacga cgatcggcag
     6661 ctcctcctcg agctgctcgc gatagcgcgt cagctcgggg tgattgcact cgaccagcag
     6721 cgtacgcacc ttggcgcgcg gggcagaacg cgaagccggc gcctgcccgg cgatggtcgc
     6781 ggtcagctcc tcgtgggtga agcccaggcg ccgcgtccgc tcgagcgtct cctgcacgag
     6841 ccgctcgagg ctgcgcgcgg cccggccttc gtgagcgggc ggccggtcgg ccacgaacgt
     6901 gccgcggccc tggtggctct ccaggtaccc gtcctggtcg aggtcggcca gtgcgcgcgc
     6961 caccgtgttg cggttgatcc ggagaaaccc cgcgagctgg cgcacggtgg ggagctgcat
     7021 ccccggcttc agcgacccga tgttgatcag gtgccggatc tgggtctgga gctggacgtg
     7081 aacgggcacc gcgctcttcc ggttgacccg gagccccgag ggcgggggga tctgcgaggc
     7141 ttcgcgggcc acgtccgtgg ctcccgtcag gcggcagctc gtcgcggtcc ggcggccagc
     7201 ttgggcgccc gcccggccac ccagtgcccg accttgatga gcgcgaagcc gatggcgacc
     7261 cgcacgggcc ggcgcgccgg cccgagcttt cggaggaggg ccagctgggc cgccgtcgcc
     7321 cgggcctcgt ccaaccgctc tctgaccagc tgctcgtaca ggtgcgtatt catcgccatc
     7381 ctcccttgat ggttgggacc ggctagtgtc ctaggacaat tcgatgccgg acccgaatat
     7441 gaaagattta gtgtcctagt aggctaagac actatatccg gcctgtcaag cttttcaagg
     7501 gtccggagct actcggcggc caggcggtag ccgacgcccg gctcggtcag cagataccga
     7561 ggtcgggcgg gatcacgctc gagcttgcgg cgcagggccc ccatgtagac ccggaggtag
     7621 tgggcctgat cggtatgggc gggcccccac acctccttta ataggtgccg ctggctcagg
     7681 acccggccgg cgtgcttgac cagggtggtc agcagccggt actcgatcgg ggtcaggtga
     7741 acgtccttgc cgtcgagctg gacctggcgc ttgacctggt ccacccggag gtctcccacc
     7801 gtgaaggtgg gctcgcccga ctcgcccccc gccacgtggc ggagcgcgac ccgcaggcgc
     7861 gcgagcagct cgcctaccgc gaacggcttg ctgacgtagt cgtcggcgcc ggcatcgagc
     7921 gccgcgacct tgtcgcgctc ctggccgcgg gcggagagca cgatgatcgg cacccggctc
     7981 cattcccgca ggcgccggat caccgccagg ccgtccaggt cggggaggcc caggtccagg
     8041 atgacgacgt cgggctggcg cgcggccgcc tcgatcagcc cgtcctcgcc ggacgttgcc
     8101 tcgtacaggc gatagccctg ggtcccgagg gtggcgcgca ggaaccggcg gatctggggc
     8161 tcgtcctcga tgaggacgat taccggcgga aacaccggcg cgggtgcgct cgcgaccggc
     8221 tcagccatcg tgctcgctga ggatcggcgg ggcttccttg accgggagcg tgaagcgcac
     8281 gcggacgccg ccgccggggc ggttctcggc ccagatatgc ccgccgtgcg cgttgacgat
     8341 gccctggcag atcgcgaggc cgagcccggc gccccgctgg ctgggagcgg cctcggcgcg
     8401 gtggaatttc tcgaagatgc gccgctcctc gcccggccgc agccccgggc ctcgatcggc
     8461 gacctcgatc agcacggcgc cgcctccgtc ctccgcgctc acctcgatcg ggctcccggc
     8521 cggcgtgtac ttgagcacat tgtcgaggag gttgatcagc acctgctcga tcagcacgtc
     8581 gtccatcggg acgagcggca gctcgggggg cacgcgggtg gtgaccgcgc gccgcgcgag
     8641 cgccttgccg aagcggccga gcgcggcccc gacgacctcc tcgacgggat gccactcggt
     8701 gtgcagctgc agcgcgcccg actcgaggcg cgtcatctcg aggaggttct gcactaggcg
     8761 gttcagccgg tcggcctcct cgtgcaggga ctccagcaga tcacggcggg tgctcgcgtc
     8821 gagccgcgcc tcgttctcga gcagggtgct ggcggcgccc gtgatggagg cgagcggggt
     8881 gcggagatcg tgcgagaccg agctcagcag cgagttacgc agccgctcgg tctcggcgcg
     8941 cacctgcgcc cgctccgctt cctcggcgag gcgggcccgc tcgatggcca gcgcggtctg
     9001 gctggcgaag gtctcgagct ggtggagctg ctccggcgcc tgcagcgggc ccggatcctg
     9061 cggccggagc ccgagcacgc ccacggtgcc gcgcgagccg gtcagcggca cgtagagcgc
     9121 ggaggcgccg gggagcgtat cggtgccctg cccggcgacc tgtccatgct cgtggaccca
     9181 ctgacccacc gccagctcgt tcgcgtccat cgggaactgc gcggcgtccc cctcgtccgg
     9241 ggccagccgg ccgtccggct gcgggagcaa caccgcgacc cgggtccgga acacctccgc
     9301 gatgtgccgg gccgccacct cccgcagctg gtgcacgccg cgcgtccccg ccagctcgcg
     9361 gctcatcgcg tagagcgcgg ccatgcgccg ctcccgctcc cgggccgact ccgcctgggc
     9421 gcggatgcgc accgcgaggc tgctgatcac cccggctacc accagcatga ccgcgaaggt
     9481 gacgagatac tgggtgtccg agaccgcgaa ggtgaagtag ggcggcacga agaagaagtc
     9541 gaaggcggcc acgctcagga gcgtcgcgag cagggagccc ccccggccgt agcgggtggc
     9601 caccatgatc acgctgagca ggtagaccat gatcaggttg gagaccgcga agtacgggaa
     9661 catggcccag gcgatcgcgg tcgaacccgc gaccgagacg acggcggcga catagggcgc
     9721 ccagtccgtc tgccgctgcc aggtacgggg gggcatgggg cgcgagtcgt cgcgatcgcc
     9781 actgatcacg tagacgtcga tctcgccgct gccctggacg agcgtgtcca cgatcgagcc
     9841 catggcgatg cgcttccaca gcgaccgctc cggcttgccc accacgatct tgctcacgtt
     9901 gcgggcccgc gcgaacgcga ggatctcgtc gctcatccgc gccccggtca aggtgtgcgt
     9961 ctcggccccg agctgctcgg ccagccgcat ggtctgggcg acccgatcgc gggcgtcctg
    10021 ggtcagacgg gtctgggcgg ctgtctccac gtaggccacg atccactccg ccccgagccc
    10081 ttccgccatg cgcttggccg cgcgcaccag gcggatcgag gacgggctcg gcccgatgca
    10141 gaccagcagc cgctcgcgca ccggccacgt ctggtggatc gcgtggtcgc gcatgtagtc
    10201 gcgcatctcc gcgtccacgc gctgcgcggt gacccgcagg gccagctcgc gcagcgcgat
    10261 caggttgccc ttgcggaaga agttctcgac ggcctcgcgg gcctggtcgg ggatgtagat
    10321 cttgccgtcc cggaggcggt ggagcaggtc gtccgggggc aggtcgatca gctccacctc
    10381 gtcggtgtgc tcgaagatgg agtcgggcac cgtctcccgc acgacgacac cggtgatctt
    10441 ggccaccacg tcgttcaggc tctcgacgtg ctgaacattc atcgcggtgt agacgttgat
    10501 gcccgcgtcg agcagctcga ccacgtcctg ccagcgcttg gcgtgacgcg agccgggagc
    10561 gttcgtgtgg gccagctcgt ccaccagcat cagggcgggc cggcgcgcca gcgccgcgtc
    10621 cagatcgaat tcccgcagcg tggtgcctcg gtactccacc aggcggcggg gcaggatctc
    10681 gaggttgccc agaagggcct cggtctcggc gcggccgtga gtctcgaccc agcccaccac
    10741 cacgtccatg ccctgggcac gctgctcgcg agccgcctcg agcatggcgt acgtcttgcc
    10801 gacccccgcg gcggccccga agaagatctt gagccggccg cgtgcctggc gggtttccgc
    10861 cgccttcacg cgggccagca gctcgtcggg atccgggcga cgctcggtgg tcatgaggca
    10921 gcctggcgtc agcctacgcc ggaagaagcg ggggatacaa ccgagggcgc gtcaagaagc
    10981 cgtcaagatt tgaggcgttc ttgacgcgga cgcgggcagt cttgagccct ccttgaccgg
    11041 tgcccctcta gcatcgaggg gggaaggaga cctggccatg gctgacatcg cgtacctcgg
    11101 aatggcgctg gcgttcttcg ggctctcgtg gctcctcgtg cggctctgtg agcggctgta
    11161 ggaggcggcc atggttctct acgtcgccgg cgccctcgtg tccgcgggcc tgctcgtcta
    11221 cctggtggtg gccctgctga agccggagct ctttcaatga ccgtgaacgc gctcttccag
    11281 ctcgttctct acatagtggt gctgcttgcc ctggctcgac cgctcggcgc ctacatggcg
    11341 cgcgtgtacg agggacgccg gctcgcgctc gaccgcgtcc tcggcgggct cgagcggttc
    11401 gtctattggg ccgccggggt atggccgaac gcggagatgg gctggaagtc gtacgcgctc
    11461 gccatgctgt ggttcaacct gctgggcgtg ctcgcggtgt acctgctcca gcggctccag
    11521 ggcgtgctgc cgctgaaccc gcagggcctg gccgcggtgt cgccggactc ggcgttcaac
    11581 accgcggtga gcttcgccag caacaccaac tggcagggct acggcggcga gaccaccatg
    11641 agctatctca cccagatgct cgccctgggc gtgcagaact tcgtgtcggc ggcttccggg
    11701 atggcgacgc tggtggcgct catccggggc ttcgcgcgcc gctccgccga caccatcggc
    11761 aacttctggg tcgacctgac ccggggcacg ctctacatcc tgctgccgct gtccttcgtg
    11821 ctcgccctgg tgctggtgtc ccagggagtc gtccagaccc tcaatccctc ggtcaccgcc
    11881 accgtggtcc agccgagcca gtacgaggtg cccgagaccg gcgcggacgg caagcccgcg
    11941 ctcgacgcga cgggtcggcc gaggatgaag acgtccacgc tgaccgagca ggtgatcgcg
    12001 gtgggcccgg ccgcttccca gatcgccatc aagcagctcg gcaccaacgg gggcggcttc
    12061 ttcaacgtca actcgtcgca tcccttcgag aacccgaccg cgctggcgaa ctttctcgaa
    12121 gtcctggcca tcctcgtcat ctcggccgcg ctctgctaca cgttcggggt catggtcaag
    12181 gacacccggc agggccggac ggtgctcgcc gccatgagcg tcatcttcgt ggtgctcctg
    12241 gcggtctgct acgtggccga gcagaacggc gccgtcttcg tgaagcaggg cgtggaccat
    12301 acggccgggg cgctccagtc ggggggcaac atggagggca aggaggttcg cttcgggatc
    12361 gccaactcgg ctctctgggc caccgccacc accgcggcgt ccaacggctc ggtcaactcc
    12421 atgcatgact cctacacccc ggtcgggggc ctcgtgccca tgtggctgat ccagctcggt
    12481 gaggtggtct acggcggcgt gggctccggg ctctacggca tgctggtctt cgccatcatc
    12541 gcggtcttcg tggccggcct catggtgggc cgcacgccgg agtacctcgg caagaagatc
    12601 gaggcctacg agatgaagat ggccgcactg gtcatcctca ttccgcccgc gatggtgctc
    12661 atcggcacgg cggtggcgat ggtcacgccc gcgggcaagg atgggatcct caatcccggc
    12721 gcccacggac tcagcgaggt cctgtacgca ttctcctcgg cggggaacaa caacggcagc
    12781 gccttcgccg gtctgagcgc caacacgccc ttctacaaca ccgccctcgg gttcgccatg
    12841 ctcttcgggc ggtactggct cgccatcccg acgctcgcca tcgcgggctc gctggcccgg
    12901 aagaagaccg tgccggtggg gccgggcacg ctgcccaccg acacgccgct gttcgccggc
    12961 ttgctcatcg gggtggtgat cctggtgggc gcgctgacct tcctcccggc gctggccctg
    13021 ggaccgatcg tcgaacatct gatcgtgatc gtcgcgaagt gaggaactga gcaatgcccg
    13081 acatggtacg agcgcgctca ctgttcgacg ccgcgatcgt caagcaagcg atcgtcgatt
    13141 cggtcaggaa gctgacggtc gtgcggcagg tccggaaccc cgtcatgttc gtggtgtacg
    13201 tcgggtccat cctcacgacc ctgctctggg tccaggccct ggtcggcaag ggcgaggcgc
    13261 cgagctggtt catcttcctg gtttcggtgt ggctgtggtt caccgtgctc ttcgccaact
    13321 tcgcggaggc catggcggaa ggccgaggta aggcccaggc cgcgtcgctt cgcaacgcgc
    13381 gtcgtgatct gcaggccaag cggctgccgc gtccggagcg gacgacccgc tacgaggccg
    13441 tctccgcctc ggccctgcgg aggaatgacc tcgtgctggt ggaggcgggc gacttcatcc
    13501 cggccgacgg gacggtcgtg gtgggcatcg cctcggtcaa cgaggcggcg atcaccggag
    13561 agagcgcgcc cgtgatccgc gaagccggcg gagaccgcag ctcggtcacc ggcggcacgc
    13621 aggtgctgtc ggactggatc atcgtggagg tcaccgccaa tccgggcgag gccttcctgg
    13681 accggatgat cgcgctggtc gagggcgcga agcggcagaa gacccccaac gagatcgcgc
    13741 tggacatcct gctcgcggcg ctgacgatcg tcttcctgct ggccaccgcc accctgctgc
    13801 cgttctccct gtacagtgtg gccgcggccg ggcagggggc gcccgtcacg gtgacggtgc
    13861 tggtcgcgct gctcgtctgc ttgatcccca ccacgatcgg cgcgctcctc tccgccatcg
    13921 gcatcgcggg gatggaccgg atgatccaga agaacgtcat cgccatgtcc gggcgggcgg
    13981 tcgaggccgc gggcgacgtg gacgtcctcc tcctggacaa gaccggcacg atcaccctgg
    14041 gcaaccgcca ggccaccgct ttcgtgcccg ccgcgggcgt caccgagacc gcgctggccg
    14101 acgcggcgca gctcgcgtcg ctggccgacg agaccccgga ggggcgcagc atcgtcgtcc
    14161 tcgccaagga gaagtacggg atccgggagc gcgagatcgc gaagctcggt gcgaccttcg
    14221 tggcgttcac cgcccagaca cgcatgagcg gcgtcaacct gaacagccgg caggtccgca
    14281 agggcgcgtc cgacgccatc gaggcccacg tcaagcatct gggcgggacc tttccgcccg
    14341 aggtccgcca gagcgtggac acgatcgcga aggcgggcgc gacgcctctc gtggtctgcg
    14401 acggagcccg ggtgctggga gccatccacc tcaaggacat cgtcaagggc ggcatccgcg
    14461 agcgcttcgc ccagctccgc cgcatgggca tcaagacgat catgatcacc ggcgacaacc
    14521 cgctcaccgc ggccgccatc agtgcggagg cgggagtgga tgacttcctg gcccaggcca
    14581 ccccggaggc caagctcaag ctcatccggg acgtgcaggc gcagggacgc ctcgtggcca
    14641 tgaccggcga cggcaccaac gacgcgcccg ccctcgccca ggccgacgtg gcgctgacca
    14701 tgaacaccgg cacccaggcc gccaaggaag ccgggaacat gatcgacctc gactccaacc
    14761 cgaccaagct gctcgaggtg gtcgagaccg gtaagcagat gctgatgacc cggggcgcgc
    14821 tcaccacgtt cagcatcgcc aacgacgtgg ccaagtactt cgcgatcatt ccggccgcgt
    14881 tcgccacgac ctacccggcg ctcggagcgc tgaacatcat gggactggcc accccggaga
    14941 gcgcgattct ctcggcggtc atcttcaacg cgctcatcat catcgccctg atcccgctcg
    15001 cgctgaaggg cgtgcggtat cgcgcgctgg gggcggccgc cgtgctaaga cgtaacctgt
    15061 ggatgtatgg cgtggggggt ctcgtcatcc ccttcccggg catcaagatc atcgacatga
    15121 tcctggtcgc cctgaagctc gtgtgagtgg aggaaccgcg atggcgcacc tgagaccggc
    15181 tctgatgtcc ctgttgctcc tcaccgtcat caccggcgtc gcctacccgc tgctggtgac
    15241 cggcgttgcc caggcggtgt ttccctccca ggccggcgga agcctgatcg tcagggacgg
    15301 gaaggtggtg ggctcggcgc tcatcggaca gcccttcgac gatccgaagt atttctgggg
    15361 ccgcccctcg gccacgagcc cgttcgccta caatgccggc gcctcctccg gctccaacct
    15421 gagcccgacg aacccggcgc tcatcgccgc ggtccaggag cgggtgggcg cgttgcgggc
    15481 ggccgatccg ggcaacacgg cccccgtgcc ggtggatctg gtcaccgcgt ccggcagcgg
    15541 gctcgacccc gacatcagcc cggcggcggc gctctatcag gtgccccgcg tggcccgggt
    15601 gcgcgggctc gcgccggacg cggtgcgcgc gctcgtcgag cggcataccg agcggcgctt
    15661 cctcggcgtg ctcggggagc cccgcgtgaa cgtgctcgcc ctgaacctgg ccgtggatgg
    15721 ccgatgagga ggcgcggacg gtcatgaacc gagccgacga cgccagggtc tggatggtga
    15781 cgggggtgct gggctccatg gccctggccg tcgcgctcat cccgctgcgt acgcaggtag
    15841 ccgcgtccaa cctcgccttc gtgttcatcg ccttcacgat catcgtggcc gagctcgggg
    15901 gtcgcggagc cgccctcgcc accgcgctga tcgccgcgat gagcttgaac ttcttcctga
    15961 ccgagcctta cctgacgctc gcgatcagca agaccgacga cctggtcgcg ttcgtcgccc
    16021 tggccgcgtg cgggctcatc gcggcggcct tcggccggcg gcgggagcgc ctctccgacg
    16081 tcgccggccg ggccgatcag gagctttcca cgctcgcccg gttcgcggaa cggtcccgga
    16141 gtgggcgtcc gccggacgcc ctcctccagg atctgcggac cgagttcgac ctgggcggtc
    16201 tcgtgctccg tgacgggtcc gggcgcgtgc tggcggcggt gcccgcggac gcggcggcgc
    16261 gcccggcgcc gcggatggcg ctcgacggca ccacgctgtt cgcggcggcc gaggagaccc
    16321 cgcgcatcgg ggccaacggc ctgcggctac ccgagggcgg gggccggttg acgctccaga
    16381 ccgcgcgcgg ccccgtgtcg ctcgacctgt gggagggcaa tgaccagggc ttcggcctcg
    16441 acgagagccg gacgctggcg atcgccgtct cgatcctcgg tctcgggatt cggtaggggc
    16501 tcgggtttcg tggtagcgtg ggttcattgc catgaagcct acgtcgctac gcgtctttgt
    16561 aacgacgctc ggcgtgctgg tggcctggcc gtcgctcgcg ctcgcggtcg gagagcccga
    16621 gcggctgtgg accgtcggcg accgggcctt ccaggacggc ctctacgggg tgtcgcgccg
    16681 catgctcgag cgcctggtcg agcgctaccc gactgacggg cgcatccccg acgccaccct
    16741 cctgctcggc aaggtgcgcc tctcccaggg gcagcacgac gcggccctgc aggcattccg
    16801 gcaggcccag accttctcgc cgccgcccgg ccggcccgaa gaggcccgct tctgggaggg
    16861 cgagacgctc ttccgcatga agcgcttccc ggaggcgcgg gccatctacg accggatcct
    16921 cgccgagagc ccgagctcgc ccagcgcccc cgacgcggtg tacgggctgg cctgggtaaa
    16981 cctcgagatg aagcggcgcg atcaggcggt caccgagttc cggcgcatcc tctcgagcta
    17041 tccggagcac gccaacgtct cgtccgcgac cttctacctg gcccgcaccc tgctcgagct
    17101 caagcgggcg gatgaggcgg tggggctcct gcgcgggttc gtggcccggt acaccgatca
    17161 ccggctgctg cccgacgcgc gctacacgct gggccaggcc ttgatcgcgg gcgggcagag
    17221 cgacgagggc gtggccgagc tgcgcgcgtt cgcgaaggcc tatcccaatc acgagctcgc
    17281 gggggcggcc cggcgtaacg tggtggacac gctcgtgcgc cagggcaaga agaacgagct
    17341 ggccgaggag tacaaggtcc tggcgtcgca gcccgccacc gccgagagcc tctacgacgc
    17401 gggtctcgtg gcgagcaaga tcgggcggcc gcgcgacgcc gacaccgcgt gggcgcggct
    17461 gcgccaggac ttccccgatc cccccctgag cggccgcgtc tcgctcgaga tggcccagtc
    17521 cgccttcgcc aagaacagct tcaaagacgc ctcgacgctg gcgcgcgcgg cgtctcgcag
    17581 cccggaggac gcggtgcgcg gcgaggcctt cgtgctgctc ggcgagagtg agctcaagca
    17641 gcgccggccc gccgctgcca tcaccgcctt caagtccgcg gcggaggcgc cggccctcga
    17701 ccccgcgatg cgcttccgcg cgctggccgg gaccggcctg gcgcacgagg agcagcggca
    17761 gtacgggccg gccgcgaagc tctacgagga ggtcgcggcc aagagcccgg acaagacgct
    17821 cgcggcctgg gcgaaggagc ggctcgccgc cgtcaacgcg gcggcccggt ccgaggctcc
    17881 gccggcggag aaggccacgc ccaagaaggc ggcgccacag aagaccgcgc ccaagcaggc
    17941 gccgggaggc acgaagtgat caggccgcgc gcgctcctgg tggccgcgct gctcgtcctg
    18001 ctgggcggcg ccaccgcgcg ggccgagatc gccctcatgc tgcccgcgcc cgacgtgggt
    18061 gggctggtgc cgctggccgc ccccgcgctc gacaagccgc ccgtgccgct gcccgtggtg
    18121 ccgctgccgc ccgcgcccca gggcatgccc gagctgccgc cggccccgat ggtggggcac
    18181 ctgggcgagc gcccggtggc cccgctgccc tcgccgcgct tcctggcctg caacccggtg
    18241 ggcaacctgc tcggcgtggc ctccgagctg atcgagtgcg ggcgggcgcg gtatcagcgc
    18301 aacgagctcg aggaaggccg cgcggtgctc gagaaggccg tgcagttcgc caccgagcgc
    18361 acgcagatcc gcgaggcgcg ctactggctc ggcgagacgc tgctgcgcct caacaagccg
    18421 gccgaggtgg agcgcctgat gctgcaggtg cagcaggacg atccgcgcgg cgagctcggt
    18481 ctctacgccg cgcaccagct cggctgggtt tccctcgagc tcaaccgacc ggaccgcgcg
    18541 ctcacctact tcgacacgct gctgaaggtc ggcgcgccgc cggccatcat ctcctacgcg
    18601 cgccacggcc gcggcctcgc gctctacggg ctcaagcgct atccggaggc gcgcgacacg
    18661 tgggccgggc tgctcaacca gagcgcgccg cgggcggtcg cgtgggaagg caccttctgg
    18721 ctcggcgaca cgctcggccg gctcggcgat cacgcggggg cggtccagcg cctccagacc
    18781 ttcgtctccg gcgggccgca ggtactgatc gacagcgggc tcctgcggct gggatggtgg
    18841 aaccgggccg ccggccagcc gctcgaggcg gcgaaggcct atcgcggcct gctctcggcc
    18901 tatccgcaat cgcgtgaggc ggcgtgggcc cgggccgggc tcgtgcaggc gctgctggac
    18961 ctcgacgact atccggccgc gcgcgaggag gccaagaagc tcgaggcgat ggatcggtcc
    19021 ggcgccctgg cggtgccggt acgcctgctg atcagccgct gggccaccga gaaggggcgc
    19081 ttcgacgagg cccgcgagat caacgacgag ctgctcgcgc gcacgctcga tcccgccacg
    19141 cgctcctacg tgctggcgct gagcgcggag gccagccggg cgagcggcca ggtcggcgag
    19201 gcccgggatc gcttcgagct cgtccggggg acgccaggct cgcccgcgct ggcggcctac
    19261 gcggcgctgc ggctcgcgca gatcgacttc gacacgcgcg agttcgccaa ggcgcgcgac
    19321 ggcgcccgga agctcgtggc cgacacggcg gcgcccgccg agatgcgcgc ggccgcgctc
    19381 gtgctgggcg gcgaggcggc ctactgggcg cgggactaca cgcaggcggc cgcggcgtac
    19441 agcggcttcc tgtcggcgta tccgggctat ccgcaggccc cgatggcggg cctcgcgctg
    19501 ggctgggccg agctacaggg cggcaagccc gaggcggcgc ggcagcgctg gacgcgctac
    19561 gcccagcagg cgcagagcga tccgcgcgcg cccgaggcgc tggtgctcgc ggcggagctg
    19621 gcgggccggg ccggagactt cgcgggcgcc cagaccctgc tcgaccaggt ggtggaccgg
    19681 tacccgaaca gcccgtacgc ggaggtggcg atgttgaacc gcgccatcct cgcggtgcgc
    19741 ggcggccgag ccgccgacgc gctgcccgag ctgggccgcc tcgtgcagcg cggggcggtc
    19801 tcgccctacg tcgcgcgcgc gcgggtcacc cgcggcgcgg cgctcctggc caccggcaag
    19861 cccgccgacg cggagcgcga cttccaggcc gcgcagagcc tgggcgagac cggggtggcc
    19921 tacctcggca tgggcaccgc ggacttcgcg cgccagcagt gggacgcggc ggtgcgcgac
    19981 ttcgtgcagg cgcgcgacgc gggcgccggg ccggtgtcgg ccgccgcgag ctacggtctc
    20041 gccgcggtgc tgttcaacca gcgccggctc gacgagttca agcaggccgc gaccccgatc
    20101 gtcgcgggtg cggtggaccc gatcatcacg ccgcgcctgc tgcacgggct ggccgcggtg
    20161 gcgacggagg agaagcgctg gcccgaggcg aagacgctca cgctgcggct ggccgaccag
    20221 ttcccgcgct acgagggcac ccaggccgcg ctgacgacgc tcgcgacggc ggcgggccag
    20281 ggtgagcagt ggatgctctc gcgcgagatg tacgccaagc tcggcgagcg cttcccgggc
    20341 agcgccaccg ccggcgccaa ccggctcgac tacgccgagg ccctgctgcg caccggcgcc
    20401 gccgcggagg cgcggcgcgc gctggagccc gccgccggcg cggcgaccgc gccggccccg
    20461 acgccgcgcg ccaccatgct gatggcggag gcgcaggaag cgacgggtga tcgcgtcgcc
    20521 gcgctggaca cctacgtgcg cgcggccgcc gcggcgggca gcccgaagga gaaggccctc
    20581 ggcctgctgg ggcaggggcg actgctggtg gccgacggca agtgggacga ggcgcgaggc
    20641 cggctcgagc aggccctcga cgcgggcgac ggcccggtgg ccgcggaggc cgcctaccgg
    20701 ctcggcgagg gctaccgcgc aacgggcaag gcgcaggatg ctgcggacat gtacatgacc
    20761 gcggcgtacc tcgccaattc gccgtgggcg cgccgcgcgc tggtcggcgc cgggcaggcc
    20821 ttcgccgcgc tcaagcagaa cgacgcggcg gtgatcgtgt atcgcaagct gctcgcgatg
    20881 agcggcgtgg agcccgatct ggccgaggcg gcgcgccgcg ggctgcgcgc gctgggcgcc
    20941 agctaggcca ccctcaccct gtcccctctc cagaggggag cgcttcgacc acctcgtcga
    21001 gatgcgggtg gtcgcccgcg tagcggtcgc gccactcgta gcgcacgcgg tctcccggcc
    21061 ccagcacgaa ggtgccgccc tgctggatca cgtcgccctg ccggcccacc tggcggaagc
    21121 cctgcgccat cgcccgcacg cccttcagca ccgcgagcgg atgataggtg ctggcccgcc
    21181 cgcgcacgag gcccgcggtc ttgaacgccc gcagcgcggg atccacgaac agcgcgccct
    21241 tgtagccggt cctctcgcgg aagcccgcga tatgctcggg cttggcgggc cccaccacca
    21301 cgagcatgcc gccgcgtcgc tcgatctccg ccgctcgttt cgacaggcca gcgacctgct
    21361 ggccgcagaa cactcagccg aagtgacgga cgaaggcgag cacgaccgga cgcttgatcc
    21421 agaagcttcc cagccgcacc tcggctcctg tctcgtccag gacgctcatc tggccgaggg
    21481 cggcggcgag cagatcgtcc atcccgtcat tgtaaccggc cctcaatctc tctaaaatgg
    21541 gcgcccatgc gaggctacgg cggccggatc ctgttcgtgg acgtgaccga cggccgcagc
    21601 cgcgtcgagt ccgtctcccc ggagcttgcg cggagcctgc tgggcggcaa cggcttcgcg
    21661 gcccggctcc tgctcgatca cgtgccggcg ggcgtggacg cctatgatcc cgccaacgcg
    21721 gtgatcttcg cggtgggccc gatcaccgac accaccgtgc ccggcaacag ccgcgcctgt
    21781 gtggcgggca agtcaccgct caccgggctc ttcttcgact cgaccttcgg cggccgctgg
    21841 cccgccacca tgaagcgcac cggcttcgac gccatcgtga tcaccggccg cgccgcggag
    21901 ccggtgtacg tcaaggttac cgaggcgggc gccgagctca agccggcgcg cgggctctgg
    21961 gggaagacca cgcgcgacac ggtgcacgcc atccaggcgg tcgagggcgg cgacgtggac
    22021 gcgatggcca tcgggcccgg cggcgagaac ctcgtgcgct tcgcggcgat gggcacctac
    22081 tggaagaatc gcgaggcctt cgcgggccgg ggcgggctcg cggcggtgct cggcagcaag
    22141 cgggtcaagg cggtggtcgt ggcggggtcg cgcaagacgg aggtggccga cgcggccgcg
    22201 ctcaagagcc tgctggagca gcagcgcgag ccgctcaaga ccgggaccaa ggcgctctcc
    22261 acctacggga cgccgttcct ggtcaagccg atcaacaccc tgggcgcgct cggctcctac
    22321 aacctgcggc aggaagtctt cgcggacgcg atggcgatct cgggcgagga gatgcacgcg
    22381 cacttccacg acaaggacac cacgtgtctc aagtgcccgg tggcctgcgg caagcaatat
    22441 gagatccgcg agggcgagct ggccggtctc aaggcgaaga tgccggagta cgagacgatc
    22501 ttcgcgttcg gctccatgct cggcaatggg cacgcggcct cgctcgcgcg ggccaacgat
    22561 ctctgcgatc tgctcgggct cgacaccatc agcatgggcg tcacgctggc cttcgtgtgc
    22621 gaggcgctcg agcgcgggtg gctcaccgag tcggagatcg gcctcgcctt cggctggggc
    22681 gactggcgcg gcatgctcaa gctcgtcgag atgacggcgg cgcgcgaggg cttcggcgac
    22741 cggctcgccg agggcggctg gcgcctcgcc gagtcggtgc acgccgaggg tacccgcctg
    22801 gtctacgcgg tcaaaaggct cgagctgccc gcccactcgg cgcgcgcgct caagggcatg
    22861 tccatcggct acgccaccgc cacgcgcggc gggagccatc acgacacgcg gcccacgccg
    22921 cagtacgcgc agaactacga ccgccgcagc cccgagggca agcccgagtt cgcggtgcgc
    22981 agccagcact tcaccgcggt ggacgattcc ctcgtgctct gccgcttcac ctcc
//
LOCUS       DASUFA010000278         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1312682, whole genome shotgun sequence.
ACCESSION   DASUFA010000278 DASUFA010000000
VERSION     DASUFA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312682"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /gene="dnaG"
                     /locus_tag="VFX87_10460"
     CDS             complement(<1..1895)
                     /gene="dnaG"
                     /locus_tag="VFX87_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX4995384.1"
                     /translation="MPGGYSQQLLDEIRSRADILEIVGQVVKLKRTGENWKGLCPFHS
                     EKTPSFTVNPKRNIYHCFGCGAGGDAFSFLMRQERVAFPEAVRTLADRAGVTLPDAGQ
                     RAPEVDGKLEALRRAMALAAEFYTRSLWEPGGEKPRAYLEQRGVDPEVAKRFGLGYAP
                     ESWNALLGVMARQGIGEEVLVQAGLILSRQNGPGFYDRFRGRLLFPIRDVQGRVVAFG
                     GRALSSEEPKYLNSPETPLYVKGQMLYALDVARIAMRERSRAIIVEGYLDCLMAHQHG
                     FGETVAALGTAFTPAQLGLLRRYADEVLALFDADAAGQKASTRLEEMMNDVMDLQNLG
                     WSVSRTGGFEKAGHLPIRVALLPPGHDPDSLLRAEGAAALTVRLDAARPLLSFVLEKA
                     FADEDLTTARGRANAHARVALILSKVANAEEATELAREAARRLGVDSTQLWIEAQQLQ
                     GARARGRRPEQLAQSGHGTAESDSWPPPVPFERDLVALLLQVDEARAELLPVLTDEDV
                     VHQGLRLVLTALRRTPGALPEALMAELPGDRERGLLAALLVEDRKWGDTHSHVAELKR
                     RYHIRHRKHRVRQVERAITDAQATGDPALPALEDELRALLRDLQREAEAVRELALARP
                     ALPSEWPV"
     gene            complement(1907..>2189)
                     /locus_tag="VFX87_10465"
     CDS             complement(1907..>2189)
                     /locus_tag="VFX87_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX4995385.1"
                     /translation="RSRITTTYKSAVGGELNIIGRTTDEARDLLEKYLDDAFLAGLAS
                     VRIIHGKGTGALRRAVEELLSAHPLVAEHRPGVSSEGGAGATVATLTQG"
BASE COUNT          305 a          778 c          784 g          322 t
ORIGIN      
        1 accggccact cgctcggcag cgccgggcgc gccagggcca gctccctgac cgcctccgcc
       61 tcccgttgca ggtcacgcag cagcgctcga agctcgtcct cgagcgcggg cagcgccgga
      121 tctccggtgg cctgggcgtc ggtgatcgcc cgctccacct ggcggacccg gtgtttacga
      181 tgccggatat ggtaccgcct cttcagctcc gcgacgtgac tatgtgtatc gccccacttg
      241 cggtcctcga ccagcagggc cgccaggagc ccccgctcgc gatctcccgg gagctcggcc
      301 atcagcgcct cgggcagcgc accgggtgtc cgccgcaggg cggtcagcac gaggcgcagc
      361 ccctgatgga ccacgtcctc atccgtgagc acggggagaa gctcggccct ggcctcatcg
      421 acctgcaaga ggagggccac gaggtcccgc tcgaagggga cgggaggcgg ccaggaatcc
      481 gattccgccg ttccgtgccc ggattgagcg agctgctcgg gccgccgacc ccgggcgcgg
      541 gcgccctgca gttgctgggc ctcgatccag agctgggtgg agtccacgcc gagtcgccga
      601 gcggcctccc gcgccagctc ggtggcctcc tcggcattgg ccaccttgga gaggatcagg
      661 gccacgcggg catgggcatt ggcccggccc cgcgccgtgg tcaggtcctc gtcggcgaag
      721 gccttctcga gcacgaagga cagcagggga cgcgccgcgt cgagccgcac ggtgagcgcg
      781 gcggcgccct cggcgcgcag caggctgtcg gggtcgtggc ccggcggcag cagcgcgacc
      841 cggatcggca gatggcccgc tttctcgaag ccgccggtgc gcgagacgga ccagcccagg
      901 ttctgcagat ccatcacgtc gttcatcatc tcctcgaggc gcgtcgaggc cttctgcccg
      961 gccgcgtcgg cgtcgaagag cgccagcacc tcgtcggcgt agcggcgcag caagccgagc
     1021 tgggccggcg tgaacgcggt gccgagcgcg gccaccgtct cgccgaagcc gtgctgatgc
     1081 gccatgaggc agtcgagata gccctcgacg atgatcgcgc ggctccgctc ccgcatcgcg
     1141 atcctggcca cgtcgagggc gtagagcatc tggcccttca cgtagagcgg ggtctccggc
     1201 gagttgaggt acttgggctc ctcgctcgag agcgcgcggc cgccgaaggc caccacgcgg
     1261 ccctggacgt ctcggatggg gaacaggagc cggccgcgga agcggtcgta gaagccgggc
     1321 ccgttctggc gggacaggat gagcccggcc tgcaccagga cctcttcccc gatcccctgc
     1381 cgggccatga cgcccagcag cgcgttccag ctctcgggcg catacccgag cccgaagcgc
     1441 ttcgccacct ccgggtccac gccgcgctgc tcgaggtagg cgcgaggctt ctcgccgccg
     1501 ggttcccaga gcgagcgggt gtagaactcg gcggcgagcg ccatcgcgcg ccgcagcgcc
     1561 tccagcttgc cgtccacctc gggcgcccgc tggcccgcgt cgggcagggt gacgcccgcg
     1621 cggtccgcca gggtgcgcac ggcctccggg aaggccacgc gctcctggcg catgaggaag
     1681 ctgaaggcgt caccgcccgc gccgcagccg aagcagtggt agatgttccg cttcgggttc
     1741 acggtgaagg agggcgtctt ctcggagtgg aacgggcaga ggcccttcca gttctccccg
     1801 gtccgcttga gcttcacgac ctggccgacg atctcgagga tgtcggcgcg ggagcggatc
     1861 tcgtcgagga gctgctggga gtatccgccc ggcattggaa gtggtctcag ccctgggtga
     1921 gggtcgcgac cgtggcgccg gcgccgccct cggaggacac ccccgggcga tgctcggcca
     1981 cgagggggtg ggcgctcagc aattcctcga ccgctcgacg gagggcaccc gtgcccttgc
     2041 cgtggatgat acggaccgac gcgagcccgg ccaggaaggc atcgtcgagg tacttttcta
     2101 gcagatcgcg ggcctcatcg gtagtccgtc ctatgatgtt cagctcccca cccaccgcgc
     2161 tcttgtaggt ggtggtgatg cggctgcgg
//
LOCUS       DASUFA010000279         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1443329, whole genome shotgun sequence.
ACCESSION   DASUFA010000279 DASUFA010000000
VERSION     DASUFA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443329"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..586)
                     /locus_tag="VFX87_10470"
     CDS             complement(77..586)
                     /locus_tag="VFX87_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4995386.1"
                     /translation="MRLEVIRRELAQGAETIQGLVVGVSAAEARVRPSPEAWSILEVV
                     AHLLDEEREDFRPRLDLVLHRPHESWAPIDPPAWVTARGYNDRELGATLSAFLTERER
                     SLAWLSSLTAPDWSREYRASFGPITAGDLAASWAAHDLLHTRQLLELRRARLLALAAP
                     HRTGYAGDW"
     gene            complement(601..1779)
                     /locus_tag="VFX87_10475"
     CDS             complement(601..1779)
                     /locus_tag="VFX87_10475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4995387.1"
                     /translation="MFPLPPLTDEQRSVVDRVATLSRERFAARAGRYDADSTFPYENY
                     ADLHAAGLFALTVPREYGGVGADPVTYVHALREIAKGCSATALTFNMHSTVTTFVAAL
                     GTEEQKRRYFGEVVGRGARIASITSEPEQSFRDKFVLNTVFHPTDGGGYRVAGVKQFC
                     SLGDAADYFFITGIQAGKTSARDGVISAMIPGRDAGVKIEGTWNATGMRGTISHTIRY
                     DCAVDRANVIGAPGQYLTIDLQGFALGYAAVYLGIGEAAFEYMTEYARTKIQRPSTEP
                     MSHHPLVQRTVAEVGTQIRAARLLLHEAACVKMAGDREATMLAVNQAKTFAADVGLMA
                     TERAMRLAGGRGILKELPLERWHRDSLAGPVMPPSNDRCLETAGKLLCGLRAATLEFQ
                     "
     gene            1930..2745
                     /locus_tag="VFX87_10480"
     CDS             1930..2745
                     /locus_tag="VFX87_10480"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX4995388.1"
                     /translation="MAPARPDFLILGLAAAGFAVSAYLTWLKWMGGNAAFCLSGSGCD
                     IVQGSRYGSLLGVPTALWGVLLYTALGALAALGLTARRWTWAFYLAAAGVGLSLYLTA
                     VSVFVIQATCAYCLASSAITVAVLVVLLSRRAVLPGRRKLSALVPGAVAAAILAPLAA
                     AFIYAMPAGGGTGFEGALARHLRETGAVMYGAHWCPHCADQKALFGDAAKDIPYVECA
                     ADAVNGRPDLCEKAGVKVFPTWVIGAERREGVQSLSALATLSKFESGTAKPTQ"
BASE COUNT          367 a          955 c         1036 g          417 t
ORIGIN      
        1 cgtggtccga tcgtgcggac cgatgcccga gagaacgagc agaacggcgc cgacgatcag
       61 gaggaggagc ggctccctac cagtcacccg cgtacccggt gcggtgcggt gcggcgaggg
      121 cgagcagccg ggcgcggcgc agctcgagga gctggcgcgt gtggagcagg tcgtgcgcgg
      181 cccaggaggc ggccaggtcg cccgcggtga tcggcccgaa ggaggcccgg tactcgcggc
      241 tccagtcggg ggcggtgagg ctcgagagcc aggccagcga gcgctcccgc tcggtgagga
      301 aggcgctcag ggtcgccccg agctcgcggt cgttgtagcc gcgcgcggtg acccaggccg
      361 gcgggtcgat gggcgcccag ctctcgtgcg gccggtgcag gaccaggtcg aggcgcggac
      421 ggaaatcctc gcgctcctcg tcgaggagat gcgcgacgac ttccaggatg gaccaggctt
      481 cgggcgacgg ccgcacacgc gcctcggccg ccgagacccc caccacgagg ccctggatgg
      541 tctccgcgcc ctgggccagc tcgcgccgga tcacctcgag tcgcatgtcc cgggtggccg
      601 ctactggaac tcgagggtgg ccgcgcgcag cccgcagagg agcttgcccg cggtctcgag
      661 gcaccgatcg ttggaggggg gcatcaccgg gccggccagc gagtcgcggt gccagcgctc
      721 gagcgggagc tccttgagga tcccgcgccc gcccgccagc cgcatggccc gctcggtggc
      781 catcagcccc acgtcggccg cgaacgtctt ggcctggttc accgccagca tggtggcctc
      841 gcggtcgcct gccatcttca cgcaggcggc ttcgtgcagg aggagccggg ccgcgcggat
      901 ctgggtgccc acctcggcca ccgtgcgctg cacgagcggg tgatggctca tgggctcggt
      961 ggagggccgc tggatcttgg tgcgcgcgta ctcggtcatg tactcgaagg cggcctcgcc
     1021 gatgcccagg tagacggcgg cgtagccgag cgcgaagccc tggagatcga tggtcaggta
     1081 ctggccgggt gcgccaatca cgttggccct gtcgaccgcg cagtcataac ggatggtatg
     1141 gctgatggtg ccgcgcatgc cggtggcgtt ccaggtgccc tcgatcttca cgccggcgtc
     1201 gcggcccggg atcatcgcgg agatcacgcc gtcgcgcgca ctggtcttgc ccgcctggat
     1261 gccggtgatg aagaagtagt cggccgcgtc gccgagcgag cagaattgct tcacgccggc
     1321 cacccgatac ccgccgccat cggtcgggtg gaacacggtg ttgagcacga acttgtcgcg
     1381 aaagctctgc tcgggctcgc tggtgatcga ggcgatacgg gccccgcggc ccacgacttc
     1441 gccgaagtag cgtcgcttct gctcctcggt gccgagcgcg gccacgaagg tggtcacggt
     1501 cgagtgcatg ttgaaggtga gggcggtggc cgagcagccc ttggcgatct cgcgcagggc
     1561 gtgcacgtag gtgaccggat cggcgccgac ccctccgtac tcgcggggga cggtgagcgc
     1621 aaacaggccg gccgcgtgca ggtccgcgta gttctcgtac gggaacgtgg aatcggcgtc
     1681 gtaccggccc gcgcgcgccg cgaagcgctc gcgcgagagg gtcgccacgc ggtcgaccac
     1741 cgagcgctgc tcatccgtca ggggcggaag cgggaacact gggctgggtc ctcctggacg
     1801 cctagggtcc cgctagcgta gcgcccctac ccgcgccggc gcaatctggg tgtccggccg
     1861 gggccggccg gcgggacctt gtggtactgt aacgtcggta tggccgcacg aaaacgcgag
     1921 gttgcccccg tggcccccgc ccggcccgac ttcctgatcc tgggcctggc cgccgccggc
     1981 ttcgccgtct ccgcctacct cacctggctc aagtggatgg ggggcaacgc cgccttctgc
     2041 ctgtcgggca gcgggtgcga catcgtgcag ggcagccgtt acggcagcct cctgggcgtc
     2101 ccgaccgcgc tctggggcgt gctgctgtac acggccctcg gcgcgctggc cgcgctcggg
     2161 ctgaccgcgc ggcgctggac ctgggccttc tatctggccg ccgcgggcgt gggtctctcg
     2221 ctctacctca ccgcggtctc ggtgttcgtc atccaggcca cctgcgccta ttgtctggcg
     2281 tcgagcgcga tcacggtggc cgtcctggtc gtgctgctca gccggcgcgc cgtcctgccg
     2341 ggccgccgga agctctcggc gctcgtgccc ggggccgtgg ccgcggcgat cctggcgccg
     2401 ctcgccgccg ccttcatcta cgcgatgccc gcgggcggcg ggaccggctt cgagggtgcg
     2461 ctggcgcggc acctgcgcga gacgggggcg gtgatgtacg gggcacactg gtgcccgcac
     2521 tgcgccgatc agaaggctct cttcggcgac gcggccaagg acattcccta cgtggagtgc
     2581 gctgcggacg cggtcaacgg ccgccccgac ctgtgcgaga aggccggcgt gaaggtcttc
     2641 ccgacctggg tgataggcgc cgagcgccgc gagggcgtgc aatcgctcag cgcgctggcc
     2701 accttgtcca agttcgaatc aggaacggcg aagcccaccc aataaccctc tcccctcggg
     2761 ggagagggca gggtg
//
LOCUS       DASUFA010000280        10279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_289604, whole genome shotgun sequence.
ACCESSION   DASUFA010000280 DASUFA010000000
VERSION     DASUFA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289604"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VFX87_10485"
     CDS             <1..1476
                     /locus_tag="VFX87_10485"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX4995389.1"
                     /translation="SEAPADVLVLGQYGEGARPAPEVAALDRKLGGLLSRVLKGEKFE
                     GKPGQISYFHTAGAVPAERVLVVGLGPLKRGARARDAEPVRRATAAAVRRARDLGAAA
                     VAVFMPAAALSARERAQAVVEGALLGTYRFDKYQKEKNGKAVRALTVLEPDRRGAAAA
                     RDGVRLGRIAAEATCLARDLVNEPANVVTPTFLARRAAEIAEESGLTLSVLDRAECAK
                     MGMGAYVGVAQGSEEPPKFIHLTYTPKGRARRRVVVIGKGITFDSGGLDLKTADGMLR
                     MKDDMSGAAAVLGIFHALARLKPRVEVHGLIAATENMPSGTAQRPGDIVRAMNGLTIE
                     IGNTDAEGRLTLADALAYAVEHIKPDEMIDMATLTGAVVIALGQGVSGMMASHDGLAS
                     RVLAAADGAGERMWRLPLHDEYKDGLKSDVADLNNISSQRGAGAIVAGLFMREFTAGV
                     PWAHLDIAGTAFAERELPLGPKGATGVGVRTLLAYLADGQR"
     gene            1492..2337
                     /locus_tag="VFX87_10490"
     CDS             1492..2337
                     /locus_tag="VFX87_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX4995390.1"
                     /translation="MAGGVDLHTHTIASDGTLRPRELVAEAARRGVRVLAITDHDSTE
                     GLAEAIEAARSHPPLVIVPGIEINCDVEGAEIHILGYYMDYEAPWFQDFCRAQREERR
                     ARVYRMAERLAELGMPIDVERVFALVQEGSAGRPHVARVMVERGYVKTVREAFDRYLA
                     AGKPAHVPRKKLTPEDAVRLLRRAGGVPVFAHPGLSDRDAMIPSLVAAGLMGIECFYT
                     EHSPAQRGSYVELCRQHDLLATGGSDFHGPPVRAAVLGNPPVPLAVWEALRVKAAEAR
                     ASRPA"
     gene            2427..2771
                     /locus_tag="VFX87_10495"
     CDS             2427..2771
                     /locus_tag="VFX87_10495"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4995391.1"
                     /translation="MRYLLLICSDKNQAPPSAAEMEAMVQGHGRFAQELRASGKMVHG
                     ERLRPDADGSRVRFKAGQRQVMDGPFAETKEALGGFYLIECDTKQEAMEWAKKVPLGE
                     GGFIDVRPIWAM"
     gene            2881..4137
                     /locus_tag="VFX87_10500"
     CDS             2881..4137
                     /locus_tag="VFX87_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4995392.1"
                     /translation="MRARLAEVFRLEHARVVASVLRIVRDIDAAQEVVQEAFEQALDH
                     WPADGIPERPGAWLLTTARRRALDHLRRARRAGARVDALAHAALGAAHPAPDVADPDE
                     IPDDRLRLIFTCCHPALPADSRVALTLRLVGGLSTTEIARAFLVPEATIAQRLVRAKR
                     TIRDGGFPYEVPDGVELASRLSAVLAVLYLIFNEGYAAHSGGDLVRHDLCEEAIRLGH
                     VLAELMPREAEILGLLALMELQASRAAARTDADGNLVLLENQDRLRWDRARIARGLAC
                     LERAGPLARAGAYQLQAAIAARHAQAGSWAATDWPRIVAHYRALAELAPSPVIDLNLA
                     VAIGLADGPAAGLAALDAIADPALRAYHLLPAARAEFLRRLGRAREAAVEYRRALQLA
                     DNPREQAFLAARLAACEAGGGSSPDG"
     gene            complement(4197..5048)
                     /locus_tag="VFX87_10505"
     CDS             complement(4197..5048)
                     /locus_tag="VFX87_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995393.1"
                     /translation="MAAAATLEADGRGRFLAPPLLQSWPGIVHGGGLVALLDLAAAAL
                     GPDGRPRLIEGRLTSSVPVDTALALEGHTDAGAATVTILDGTQPLTSATIGHGDIDDP
                     APDPGWRATGREGWPMPLSDQCLACGAHNPLGLQLGLRFDGDGVWARFLPREPWRRSG
                     DRLAPALPAVVLDEIAWWLGALVTREGGLTNRIALTLVRPEAIAPGPVTAVGRFADVT
                     PVDRRRTFWRTRSALLAADGTLLATAAIVFRSGADYSERQMAFFRSRTDPGIFERMFP
                     NHLEGDR"
     gene            5160..6377
                     /locus_tag="VFX87_10510"
     CDS             5160..6377
                     /locus_tag="VFX87_10510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995394.1"
                     /translation="MTSSTLELLSHGPFARIMLTNFFFFGALNTFLLLPLYIQRLGGD
                     AADIGLVQGLYSAAGIVCQPVVGALIDYLGRRFFMRLGTLLLVASCALFVVSSPLPLL
                     GVLRILQGVAFSAFFVANYIHVVEMVPAERRGWALGIFGLSGLVSTSLAPLFGEFVIR
                     RFGFPTFFMTSTVIAAVATAACWRVRDVRPAALGARPGLHVIREGLLELRHMHMLIGL
                     FFGLGTGTIFTFLPTFGEDLGIASVGLFYTAYAGAAMLVRVVGGNLIDVRGRRAVIIP
                     CMFVQAAATCIIALVAVLFAPSLRLPVLPFIFLAGFLAGAAHGFLYPAMSALLMDVTP
                     ERRRGSAVGIFSSVILVGNSFGAIAFGYVAHGLGYAVMWSGLTVLLIVGSFLSVRLRV
                     GYARPVAAPAPAS"
     gene            6467..6997
                     /locus_tag="VFX87_10515"
     CDS             6467..6997
                     /locus_tag="VFX87_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX4995395.1"
                     /translation="MVRRSRYRRLVPVLALGVLLAGAGNPGETPAPPATSPQEVTVIG
                     VFSDQRGQHSVMLEGKRDKRRFLMGIGAAEATAIAVPLENITPPRPLTHDLFLTLFGR
                     LKVQVTKVVITDLRSNTYYATVYLASGETAMELDSRPSDAIALAIRAKVPVFAEDRVF
                     EKSDRPGSPGGPGERI"
     gene            7002..7988
                     /locus_tag="VFX87_10520"
     CDS             7002..7988
                     /locus_tag="VFX87_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4995396.1"
                     /translation="MANETPRAGTPALPPPGAQNVEGQQWAIERRLGEIFGKLGEKLA
                     DLTGRVEVRVKPETSFTDIGGLAEAKGIVRSFVTALTDPELYRKWGISPPKGLLIYGP
                     PGTGKTLLARALATEAGAVFYHLKLGTLTSKFGPNTGELLQEILGLAREQGRAVVFLD
                     HADALALEHLLPPAQAREAGARIVAALCEKLDSLEDFSRIVVVAATSRTDAVDPSLVA
                     PGRLDRLVEAPMPDAAAQQQILELTRARAEHAADRRLVGDLDYRSLLPPMGGMSGGEL
                     RDLLRRALEEKVHAAGQGQDAGLVSTQDLLRQVDVYRRIRAVVEKIRYGQYL"
     gene            8007..8753
                     /locus_tag="VFX87_10525"
     CDS             8007..8753
                     /locus_tag="VFX87_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX4995397.1"
                     /translation="MRTRRGLRHWSGWLAVAAVLFVLTGPASAQWDKLLKGLGGGEGS
                     AGAGLSDAKIGAGLKEALQVATEKTVSLTGKTDGYFANQAIKILMPEKLRNFESGLRA
                     VGFGSQVDELVLGMNRAAERAAPKAKQILFDAIGEMSFDDARKVLNGGDTAATEYFRG
                     KTTPRLTTAFRPVVEQSMNQVGVSRQYKDLVGRFESIPFAKSQAFDLDGYVVDKGLGG
                     LFTVLGEQEKQIRVNPTARATDLLKEVFKR"
     gene            8868..9290
                     /locus_tag="VFX87_10530"
     CDS             8868..9290
                     /locus_tag="VFX87_10530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4995398.1"
                     /translation="MPSVTRVRVRYKDTDTMSVVYYGNYLTYFEVGRVEYLREYGWPM
                     SAVNERVHLPVVEAFVKYVKPARLDDLLEVTSRVSERKRASFRFSYEIRNEAKDLLAT
                     GHTVHACWNPATAKMIPIPDWLKEIMPDADLDPVTPRR"
     gene            9256..>10279
                     /locus_tag="VFX87_10535"
     CDS             9256..>10279
                     /locus_tag="VFX87_10535"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX4995399.1"
                     /translation="MPTSTPSRPDADRQPRPIAQIARELGLGVEEWIPYGHDKAKVRV
                     GALAARRDRPDGKLVVVSSITPTPAGDGKTTMTIGVGQALWRVGARPVIALREPSIGP
                     TLGMKGGGTGGGKAQVVPMEEINLHFTGDFHAITSAHNLLAAAIDNHLHHGNPLGIDV
                     RQVAWKRVLDVNDRALRSVVVGLGGRLDGVPREAGFLITSASEIMAALCLAEDLMDLK
                     QRLGRMLVALTADGKPVTADELGVTGAMAALLRDAIHPNLVQTMEGTPALIHGGPFAN
                     IAHGCNSVVATRLALKLGEICVTEAGFAFDLGAEKFLDIKCGYAGLRPDAVVLVATAR
                     ALKYHGG"
BASE COUNT         1422 a         3662 c         3749 g         1446 t
ORIGIN      
        1 tcggaagcgc ccgcggacgt gctggtgctg gggcagtacg gcgagggcgc gcggcccgcg
       61 ccggaggtcg ccgcgctgga ccggaagctc ggcggcctgc tctcgcgcgt gctgaagggc
      121 gagaagttcg agggcaagcc gggccagatc tcgtacttcc acaccgcggg cgcggtcccc
      181 gccgagcgcg tcctggtggt ggggctcggc ccgctcaagc ggggggcgcg ggcccgcgac
      241 gccgagcccg tgcggcgcgc caccgccgcc gcggtgcggc gcgcgcgcga tctgggcgcc
      301 gccgccgtcg cggtgttcat gcccgccgcc gcgctgtccg cgcgcgagcg ggcgcaggcg
      361 gtcgtggaag gcgcgctgct cggcacctat cgcttcgaca agtaccagaa ggagaagaac
      421 ggcaaggcgg tgcgggccct gaccgtgctc gagcccgacc gccgcggcgc ggccgccgcg
      481 cgcgacggcg tgcgcctcgg ccggatcgcg gcggaggcca cctgcctcgc gcgcgacctg
      541 gtcaacgagc cggccaacgt ggtgacgccc accttcctgg cgcggcgcgc ggccgagatc
      601 gccgaggaga gcgggctcac cctcagcgtc ctggaccggg ccgagtgcgc caagatgggc
      661 atgggcgctt acgtgggcgt ggcgcagggc agcgaggagc cgcccaagtt cattcacctc
      721 acgtacaccc ccaagggccg cgcgcgccgc cgcgtggtgg tgatcggcaa gggcatcacc
      781 ttcgactcgg gcgggctcga tctgaagacg gccgacggca tgctgcggat gaaggacgac
      841 atgtcggggg ccgccgcggt gctcggcatc ttccacgctc tggcgcggct caagccccgg
      901 gtcgaggtgc acgggctgat cgcggccacc gagaacatgc cgtccggcac cgcccagcgc
      961 ccgggtgaca tcgtgcgggc gatgaacggg ctgaccatcg agatcggcaa taccgacgcg
     1021 gagggccggc tcacgctggc cgatgccctc gcctacgcgg tcgagcacat caagccggac
     1081 gagatgatcg acatggcgac gctgaccggc gcggtggtca tcgcgctggg ccagggcgtg
     1141 tccgggatga tggcgagcca cgacggcctc gcgtcgcggg tcctggcggc cgccgacggg
     1201 gcgggggagc ggatgtggcg cctgccgctt cacgacgagt acaaggacgg gctcaagagc
     1261 gacgttgccg atctcaacaa catctcgagc cagcgcgggg ccggcgccat cgtggccggc
     1321 ctcttcatgc gcgagttcac cgcgggcgtg ccgtgggcgc atctcgacat cgcgggcacc
     1381 gcgttcgccg agcgggagct gccgctcggg cccaagggcg ccaccggtgt gggcgtgcgg
     1441 accctgctcg cctacctcgc ggacgggcag cgctaggcgc cccggggccc gatggcgggc
     1501 ggcgtcgacc tccacacgca cacgatcgcc tccgacggca cgctccgacc gcgcgagctg
     1561 gtggcggagg ccgcccggcg cggggtgcgc gtgctcgcga tcaccgatca cgactccacc
     1621 gaaggcctcg ccgaggcgat cgaggcggcg cggtctcacc cgccgctcgt catcgtgccc
     1681 ggcatcgaga tcaactgcga cgtggagggc gccgagatcc acatcctcgg ctactacatg
     1741 gactacgagg ccccctggtt ccaggacttc tgccgcgccc agcgggagga gcgccgcgcc
     1801 cgcgtgtacc ggatggccga gcggctcgcg gagctgggca tgccgatcga cgtcgagcgg
     1861 gtgttcgcgc tggtgcagga gggctcggcg gggcgcccgc acgtggcccg cgtgatggtc
     1921 gagcgcggct atgtcaagac cgtgcgcgag gccttcgacc ggtacctggc cgcgggcaag
     1981 cccgcgcacg tgccacgcaa gaagctcacg cccgaggacg cggtgcgcct gctgcgccgg
     2041 gccggcggcg tgcccgtgtt cgcccacccg gggctgtcgg atcgggacgc gatgatcccg
     2101 tccctagtcg cggcggggct catgggcatc gagtgcttct acaccgagca ctcgcccgcc
     2161 cagcgcggca gctacgtcga gctgtgccgg cagcatgacc tgctcgcgac gggcggctcg
     2221 gatttccacg gcccgccggt gcgtgccgcg gtgctgggca acccgcccgt gcccctggcg
     2281 gtctgggagg ccctgcgggt gaaggcggcg gaagcgcggg cgagccgccc agcctgacct
     2341 ccgctgtcga ttccgggggg tcatcggcgt cgtatagatg agggccgctt cgacggcccg
     2401 accatccacg atagggaggc ataccaatgc gttacctgct gctcatctgc tccgacaaga
     2461 accaggcccc tccgtccgcc gccgagatgg aggccatggt gcagggacac ggccgcttcg
     2521 cccaggagct gcgcgcgtcc ggcaagatgg tccacgggga gcggctccgc cccgacgccg
     2581 acggcagccg cgtccgcttc aaggccgggc agcgccaggt catggacggg cccttcgccg
     2641 agacgaagga ggccctgggc ggcttctacc tgatcgagtg cgacaccaag caggaagcca
     2701 tggagtgggc caagaaggtc ccgctcggcg aggggggctt catcgacgtg cgtccgatct
     2761 gggcgatgtg agggcgggag accccgccgc cgcgccgccg acccgccggg cgcgccacca
     2821 gcatgatcgc cggatggcgt accatgtcga cttgtgggac cgcagactcc ggacgagcgc
     2881 gtgcgcgcgc gcctggccga ggtgttccgg ctcgagcacg cgcgggtggt cgcctcggtg
     2941 ctccggatcg tgcgcgacat cgacgcggcg caggaggtcg tccaggaagc cttcgagcag
     3001 gcgctcgatc actggcccgc ggacggaatt ccggagcgcc cgggggcctg gctcctcacc
     3061 accgcccgac gccgggcgct ggatcacctg cgccgtgccc gccgcgccgg ggcgcgcgtc
     3121 gacgccctcg cccacgcggc gctcggcgcg gcgcatccgg cgcctgacgt ggccgacccc
     3181 gacgagatcc ccgacgatcg gctccggctg atcttcacgt gctgccatcc cgcgctgccc
     3241 gcggacagcc gcgtcgcgct cacgctgcgg ctggtgggag gcctctcgac cacggagatc
     3301 gcccgggcct tcctggtgcc cgaggcgacg atcgcccagc gcctcgtgcg cgccaagcgg
     3361 acgatccgcg acggcggctt cccgtacgaa gtgcccgacg gggtcgagct ggcgtcccgg
     3421 ctgtcggccg tgctcgccgt gctgtacctg atcttcaacg agggctacgc ggcgcactcg
     3481 ggcggcgacc ttgtgcgaca cgacctctgc gaggaggcga tccggctcgg ccacgtgctc
     3541 gcggagctga tgccgcgcga ggccgagatc ctgggcctgc tggccctgat ggagctccag
     3601 gcctcgcgcg cggcggcccg caccgacgcc gacggcaatc tcgtgctgct cgagaatcag
     3661 gaccggctcc gctgggaccg ggcgcggatc gcccggggcc tcgcctgcct cgagcgcgcc
     3721 gggccgctgg cccgggcggg agcctaccag ctccaggccg ccatcgccgc ccgtcacgca
     3781 caggccggat cctgggccgc gacggactgg ccgcgcatcg tggcccacta ccgagcgctc
     3841 gccgagctgg ccccatcccc ggtcatcgat ctgaacctgg cggtggcgat cggcctggcc
     3901 gacgggcccg cggccgggct cgccgcgctc gatgcgatcg ccgatccggc gctgcgtgcc
     3961 tatcatctgc tccccgccgc ccgcgccgag ttcctccgcc gcctcggccg ggcgcgggaa
     4021 gccgccgtcg agtaccggcg cgcgctccag cttgcggaca acccgcgcga gcaggcgttc
     4081 ctcgcggccc gcctggccgc gtgcgaggcc ggcggaggat cctctcccga cggctagccg
     4141 gacacgcccc cgatcgtggc gacgacgtac gagccgtcgc gatcaccagg caggaattac
     4201 cgatctccct ccaggtgatt ggggaacatc cgctcgaaga tccccggatc ggtgcgcgag
     4261 cggaagaagg ccatctggcg ctcggagtaa tcggccccgg accggaagac gatggcggcg
     4321 gtggccagca gggtgccgtc ggcggcgagc agggccgagc gggtccgcca gaacgtgcgc
     4381 cgccggtcca ccggcgtcac gtcggcgaag cggcccaccg cggtgaccgg gccgggcgcg
     4441 atcgcctccg ggcgcaccag cgtcagggcg atgcggttgg tgaggccgcc ctcgcgcgtg
     4501 acgagtgcgc cgagccacca cgcgatctcg tcgagcacca ccgcgggcag ggcgggagcg
     4561 aggcgatcgc cggagcgacg ccagggctcg cggggcagga agcgggccca gaccccgtcg
     4621 ccgtcgaagc gcaggccgag ctgcagcccg agcggattgt gcgcgccaca ggccaggcac
     4681 tgatcggaca ggggcatcgg ccagccctcg cgcccggtcg cccgccagcc ggggtcgggc
     4741 gcggggtcgt cgatgtcgcc atgtccgatc gtggccgacg tgagcggctg cgtcccgtcg
     4801 agaatggtga cggtggcggc gccggcgtct gtgtggcctt cgagcgcgag cgccgtgtcg
     4861 accggcaccg acgaggtgag gcggccttcg atgagacgcg gccggccgtc cgggcccagc
     4921 gccgcggccg ccaggtccag cagcgcgacc aggccgccac cgtgcacgat gccgggccag
     4981 ctctgcagga gcggcggcgc gaggaagcgc ccgcggccgt cggcctcgag cgtcgcggcg
     5041 gcggccatga cgtcaccgat ggccgggccc gccgggcgtg cgcgggggcg cgtgctatac
     5101 tcgcgaggat tcatgccgct ggccattcta ctgtcgccgt cgctcccgcc gcaacgcgtg
     5161 tgaccagctc gaccctcgag ctcctgagcc atggtccgtt cgcgcggatc atgctcacga
     5221 acttcttctt cttcggcgcg ctgaacacct tcctgctcct gccgctgtat atccagcggc
     5281 tcgggggcga cgcggcggac atcgggctcg tccagggcct ctacagcgcg gccggcatcg
     5341 tctgccagcc ggtggtgggc gccctcatcg actacctggg ccgccggttc ttcatgcggc
     5401 tcggcaccct gctgctcgtc gcgtcctgcg ccctcttcgt ggtgtcgagt ccactgcccc
     5461 tgctgggcgt gctgcgcatc ctgcagggcg tggccttctc cgcgttcttc gtcgccaact
     5521 acatccacgt ggtcgagatg gtcccggcgg agcggcgtgg ctgggcgctc ggcatcttcg
     5581 gcctctcggg cctcgtctcc acgtcgctcg ctccgctctt cggcgagttc gtgatccgcc
     5641 gcttcggctt tcccaccttc ttcatgacct ccaccgtgat cgctgcggtg gccaccgcgg
     5701 cgtgctggcg cgtgcgggac gtgcgcccgg ccgcgctggg cgcccggccg ggcctgcatg
     5761 tcatccggga agggctgctc gagctgcggc acatgcacat gctgatcggg ttgttcttcg
     5821 ggctgggcac cgggacgatc ttcacgttcc tgccgacctt cggcgaggac ctgggcatcg
     5881 cgagcgtcgg cctcttctac accgcgtacg cgggggcggc catgctggtg cgggtggtgg
     5941 gcggcaacct gatcgacgtc cgcgggcggc gggcggtgat cattccctgc atgttcgtgc
     6001 aggccgccgc gacctgcatc atcgcgctgg tcgccgtcct gttcgcgccg tcgctccggc
     6061 tgccggtgct gccgttcatc ttcctggccg ggttcctggc cggcgcggcc cacggcttcc
     6121 tctacccggc catgtcggcc ctgctgatgg acgtgacgcc cgagcggcgc cgcggcagcg
     6181 cagtgggcat cttcagctcg gtgatcctcg tcgggaactc gttcggggcg atcgccttcg
     6241 gctacgtggc gcacgggctc gggtacgcgg tgatgtggag cgggctcacc gtgctgctga
     6301 tcgtcggttc cttcctgagc gtccggctgc gcgtgggcta cgcgaggccg gtggcggcgc
     6361 ccgcgcccgc gtcctgaccg gcgctccgag tgggcggcgc ggttgacttg ccccggacgc
     6421 ggaccgatac tgacacgcac gacgcagcgt gggaggcctc tcgatcatgg tgagacgcag
     6481 ccgatatcgc cgcctggtgc cggtgctcgc gctgggcgtt ctcctcgcgg gcgccggcaa
     6541 tccgggggag acgcccgcgc cgcccgccac ctcgccgcag gaagtcacgg tcatcggcgt
     6601 gttcagcgat cagcgcggcc agcacagcgt gatgctcgag ggcaagcgcg acaagcggcg
     6661 cttcctgatg gggatcggcg ccgcggaggc caccgccatc gcggtcccgc tcgagaacat
     6721 cacgccgccg cgccccctca ctcacgatct cttcctcacc ctgttcgggc ggctcaaggt
     6781 gcaggtcacg aaggtggtga tcacggatct gcgcagcaac acctactacg cgacggtgta
     6841 cctggcctcg ggcgagaccg cgatggagct cgactcgcgt ccctctgacg ccatcgcgct
     6901 ggccatccgc gcgaaggtgc ccgtcttcgc ggaggaccgc gtgttcgaga agtccgatcg
     6961 gcccgggagc cccggcgggc cgggggagcg gatctagccc catggcgaac gagactccgc
     7021 gcgcgggtac gccagccctt ccgccgcccg gtgcgcagaa cgtcgagggg cagcagtggg
     7081 cgatcgagcg gcgcctgggc gagatcttcg gcaagctcgg ggagaagctc gcggatctca
     7141 ccgggcgggt cgaggtgcgg gtcaagccgg agacatcgtt cacggacatc ggcggcctcg
     7201 ccgaggccaa gggcatcgtg cggagcttcg tgaccgcgct gaccgacccc gagctctatc
     7261 ggaagtgggg catcagcccg cccaagggcc tgctgatcta cgggccgccg ggcaccggca
     7321 agacgctgct ggcgcgcgcg ctcgccaccg aggcgggcgc cgtcttctac cacctgaagc
     7381 tcggcacgct cacctccaag ttcgggccca ataccggcga gctgctgcag gagatcctgg
     7441 gcctggcgcg cgagcagggg cgggccgtgg tgttcctcga tcacgccgat gcgctcgccc
     7501 tcgagcatct cctgccgccg gcccaggccc gcgaggcggg tgcgcgcatc gtggccgcgc
     7561 tgtgcgagaa gctcgacagc ttggaggact tttcgcgaat cgtggtggtg gcggccacca
     7621 gccgcaccga cgcggtggat ccctccctgg tcgccccggg ccggctcgat cgactggtcg
     7681 aggcgccgat gccggacgcg gccgcccagc agcagatcct cgagctgacg cgcgcgcgcg
     7741 ccgagcacgc agccgatcgg cgcctggtgg gtgatctcga ctaccgctcc ctcctgccgc
     7801 cgatgggcgg catgagcggg ggcgagctcc gcgacctgct gcgccgcgcg ctcgaggaga
     7861 aagtccacgc ggccgggcag ggccaggacg cgggactcgt cagcacccag gacctgctgc
     7921 gccaggtgga cgtctaccgg cgcatccggg ccgtcgtcga gaagatccgc tacgggcagt
     7981 acctctagcc cgggcgagga ggcatcatgc gcacacgtcg aggattgagg cactggtccg
     8041 gctggctggc cgtcgcggcg gtcctgttcg tgttgaccgg cccggccagc gctcagtggg
     8101 acaagctgct gaaggggctg ggcggcgggg aggggagcgc gggcgccggg ctctcggacg
     8161 ccaagatcgg cgcggggctc aaggaagcgc tccaggtggc caccgagaag acggtgagcc
     8221 tcaccgggaa gaccgacggc tacttcgcca accaggccat caagatcctg atgccggaga
     8281 agctgcggaa cttcgagagc gggctgcgcg cggtgggctt cggcagccag gtcgacgagc
     8341 tggtgctcgg catgaaccgg gctgcggagc gggcggcgcc caaggccaag cagatcctct
     8401 tcgacgccat cggcgagatg agcttcgacg acgcccgcaa ggtcctgaac ggtggggaca
     8461 ccgcggccac cgagtacttc cgcgggaaga ccacgccgcg gctgaccact gcgttccggc
     8521 cggtggtcga gcagtccatg aaccaggtcg gcgtgagccg gcagtacaag gatctcgtcg
     8581 ggcgcttcga gtccatcccg ttcgccaagt cccaggcctt cgacctggac ggctacgtcg
     8641 tggacaaggg gctgggtggg ctcttcacgg tgctggggga gcaggagaag cagatccgcg
     8701 tcaacccgac cgcccgcgcc accgatctcc tgaaagaagt cttcaagcgc tagcggattg
     8761 gggtcaggtc ttacattctg acatttcccg gcgtggaggt ggagcgaacc ctcaggaaat
     8821 gtcggaatgt aagacctgac cccaggtggt accatggcgc cgcgtcgatg ccatcggtta
     8881 cccgcgtgcg cgtgcgatac aaggacaccg acaccatgtc ggtcgtctac tacggcaact
     8941 acctcaccta cttcgaggtg gggcgcgtgg agtaccttcg cgagtacggc tggcccatgt
     9001 cggcggtgaa cgagcgcgtg cacctgccgg tggtggaagc cttcgtgaag tacgtcaagc
     9061 ccgcccggct cgacgacctc ctggaagtca cctcgcgggt gagcgagcgc aagcgggcca
     9121 gcttccggtt cagctacgag atccgtaacg aggccaagga cctgctggcc accgggcaca
     9181 ccgtgcacgc ctgctggaac ccggccaccg ccaagatgat acccatcccg gactggctga
     9241 aggagatcat gcccgatgcc gacctcgacc ccgtcacgcc ccgacgctga ccgacagccc
     9301 cggccgatcg cccagatcgc gcgcgagctc ggcctcggcg tggaggagtg gatcccctac
     9361 ggccacgaca aggccaaggt acgcgtcggc gcgctggccg cccgccgcga ccggcccgac
     9421 ggcaagctgg tggtggtgtc gtccatcacg ccgaccccgg ccggcgacgg caagaccacc
     9481 atgaccatcg gggtggggca ggcgctctgg cgggtcggcg cccgtccggt gatcgcgctg
     9541 cgcgagccgt ccatcgggcc gacgctgggc atgaagggcg gcggcaccgg gggcggcaag
     9601 gcgcaggtgg tgccgatgga ggagatcaac cttcacttca ccggcgactt ccacgcgatc
     9661 acctcggcgc acaacctgct cgcggccgcc atcgacaacc atctccacca cggcaacccg
     9721 ctcggcatcg acgtccgcca ggtggcgtgg aagcgcgtcc tcgacgtcaa cgaccgcgcc
     9781 ctgcggagcg tggtggtcgg gctcggcggg cgcctcgacg gcgtgccgcg cgaggcgggc
     9841 ttcctcatca cctcggcctc cgagatcatg gccgccctct gcctggccga ggacctgatg
     9901 gatctcaagc agcggctcgg ccggatgctg gtcgcgctga ccgccgacgg caagccggtg
     9961 accgcggacg agctcggcgt gaccggcgcg atggccgcgc tgctgcgcga cgccatccac
    10021 ccgaacctgg tgcagaccat ggaggggacg cccgcgctga tccacggggg ccccttcgcc
    10081 aacatcgccc acggctgcaa ctcggtggtg gccacccggc tggccctcaa gctgggggag
    10141 atctgcgtca ccgaggccgg gttcgccttc gacctggggg ccgagaagtt cctggacatc
    10201 aagtgcggct acgccgggct ccggcccgac gccgtggtcc tggtggccac ggcccgggcc
    10261 ctcaagtacc acgggggcg
//
LOCUS       DASUFA010000281         8890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1198770, whole genome shotgun sequence.
ACCESSION   DASUFA010000281 DASUFA010000000
VERSION     DASUFA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198770"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFX87_10540"
     CDS             <1..583
                     /locus_tag="VFX87_10540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995400.1"
                     /translation="QESVSFKGAHYRVEGRLEPKSVSRPHPPVWIGGWGDLTLRRAAT
                     LADNWIPGPTADLPRLLAGKQQFLTNRRAAGRPEAVTEWPLTRDVIIADTDREARELA
                     ERHIMISYRKEYAGGWRHPFIDASIATDLDGLMKNRFLIGGPEQVVRALRPFVDEYGM
                     THLICRVFFPGMPHRHIMRELELLSREVLLAFA"
     gene            614..1591
                     /locus_tag="VFX87_10545"
     CDS             614..1591
                     /locus_tag="VFX87_10545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4995401.1"
                     /translation="MRWLIVLPFARPGLMGMDFRDELVAMGHEVRTFAYRRDNPLYKN
                     RSTKATYQIFILRRLERACLAWRPDLVLVIKGGPVTPGLIRRVKARIDTRFLNFFPDN
                     PLWMIPFDCVEAYDAFFTKERYALGALEQAGLRNLHYLPMYCVPEQHHPIELTAEETR
                     RYAAPISFVGNAYPYRARFVRELAGYPVRLWGGGWDRVEAPEVRAMVAGGPVWGRAKL
                     AVYSGSTLSLNHHHPMNDIVGVNTRAFELAASAACQVVDAKGELAHLFTAGEEVVVYH
                     DLAELRHQLDHYLAHPDEARDIGERARKRALAEHTLRHRIEAMIAMLGR"
     gene            1603..2829
                     /locus_tag="VFX87_10550"
     CDS             1603..2829
                     /locus_tag="VFX87_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX4995402.1"
                     /translation="MRRESFATELRCIACGRAHPLDYVLECTACRGLLELAYDWEALR
                     RAGPGVFTGGGLWRYAPVLPIADPAHRVSLGEGQSPLLDCPALGRRLGVRRLQVKFEG
                     SNPTGTVKDRSSPTAVAAALQFGFRATSVVSSGNAGSSIAAYSARAGLRSLIFAYERT
                     SAPKLLHMTATATDLVLYQGVYDDLITLWDRLVEERLFFDCGASRNAYKHEGKKTLAY
                     EIAEQSGWRAPDVVVAPAAVGETFIATHRGFVELERLGWIARRPLMVAAQAARANAIT
                     RAWRGKRPLEPVKIGYTVAEGLAAGNPGRKGEWVLRILNETGGVAGEAEDEEILETQA
                     LLARTEGIWAGPTGVAALAVLRRLLAEKAIDPDATICVILSETGLKTEASAPSRRATA
                     FDEASLRRLVHSTLTP"
     gene            complement(2819..3493)
                     /locus_tag="VFX87_10555"
     CDS             complement(2819..3493)
                     /locus_tag="VFX87_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX4995403.1"
                     /translation="MKRYASRDGRLRLFVGDSRRLTGIATGSVGVILTSPPYWVRGRG
                     RARAERYARELASGFAREWRRVLADHGDLWLVIGDRHDGTEWVGMDALVTSHLRRAGW
                     RLQSKGLWAQVRSRERWDNRVNHLLRFRKAEHPVRMDGTTLCWMLPLPRSHPESLWDA
                     TPDPVLRGLLALSARRGAVLDPFAGAGTVGRIAAALGREWIGVERDPHMAALTARRLR
                     LRPVRG"
     gene            complement(3516..4295)
                     /gene="lgt"
                     /locus_tag="VFX87_10560"
     CDS             complement(3516..4295)
                     /gene="lgt"
                     /locus_tag="VFX87_10560"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX4995404.1"
                     /translation="MFQSPGAIAVHVGPIAIRWYGVLMATAMALGLWLAHRDARRRGL
                     DSERLVKASELALLGGLIGARLYYVAFNLDYYGQFPGKIFALWEGGLAIHGGVLGGIL
                     VGGVYAWRRRLPLRQYLDVAAPSLALGQAIGRWGNFFNEEAFGRPTELPWKLYISPPH
                     RPPPFAQAEFFHPTFLYESVWDLVVFGLLVFVLRDRLARAPGALFLAYVGLYSLGRLF
                     TEGLRTDPLMLGSLRVAQLVSLLCMTLALVGVPLLLRRRPI"
     gene            4458..5198
                     /locus_tag="VFX87_10565"
     CDS             4458..5198
                     /locus_tag="VFX87_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4995405.1"
                     /translation="MPGSGSANGGVAELVLSAGPVAKFVLLLLATFSVICWALIVEKW
                     WEFRKIRRESLRFRQIFREARRFSVVYGAAKKHRDSPLAQLYVAAGQELVTAYGSAEM
                     VDHVLDDADEGLAVERLDATSRALRRAGSAEVARMERYLPFLATVASSAPFIGLFGTV
                     WGIMTAFQNIGQQGSANLAVVAPGISEALVATAAGLGAAIPAVMGYNFFVNRTKHWAM
                     EMEGFTLDLLNAFARPAQKMARVPKDGH"
     gene            5188..5628
                     /locus_tag="VFX87_10570"
     CDS             5188..5628
                     /locus_tag="VFX87_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4995406.1"
                     /translation="MAIKVDTPDYGSGGSYRIGGTMSEINIIPLVDVILVLLLIFMLT
                     APLMYRGIDVNLPKSSGKPTAVEERVVLTVTKEQTIYINDKPVALGAVEQALRDLFKN
                     RQDKTLYLRADQALQYGLVVETMDRVRRAGIEKLGMVTEPTRAR"
     gene            5637..6491
                     /locus_tag="VFX87_10575"
     CDS             5637..6491
                     /locus_tag="VFX87_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4995407.1"
                     /translation="MAARRYPIGPLPDPGLRRPGPALRRRRLPLAAIGFSTVGHVVVF
                     GGMAAFVMWGGWRPEKIQVVNLVPMVAAVGNPNAPAAPTLPARPQAPVAPRAPEPEPK
                     ATTAPREAPKREAPSLPAPSLPAARPLPPRATLPRPGEKELPSLATPPAGTPRGEKTA
                     ESARPPETASLGAPTGSVTGAGALTLDVGDFPHAWYLRQVLQKVEQEWQRQNQPSDPQ
                     QKPQIYVEILRDGSIKPPAIHKSSGNAFYDNAALRAVMAAGPFPKLPDDWAKPSLRIL
                     FTFDIRRG"
     gene            6510..7859
                     /gene="tolB"
                     /locus_tag="VFX87_10580"
     CDS             6510..7859
                     /gene="tolB"
                     /locus_tag="VFX87_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX4995408.1"
                     /translation="MNKMMRPGRPLGFLVGLVLLAALGGALASPPAARPQAADVLLNV
                     LATGAKKLNIVIPHFTVVGGPDPQGLGRSLSQVTARDLTFSSLFSVVSDVPAIPVGDP
                     AALRAAFANFAAAGAHAGLQGVLTLRGDRAEIEMRLHDLTSPEFRLIGAKKIELPTAQ
                     ARRLAHKVADEVVLQFTGEAGVADTKVAYVASGSGSKELSLMDYDGASAARVTSNKSI
                     NISPVWSPDTRSLAFTSYMRGYPYLYRMFPFENRPVQVLAGFLGINTSPAFSPDGRSV
                     ALTLSRDGNPEVYVLNLASGAFRRLTTYSGIDTEPSWSPTGREIAFVSERAGGAHIFV
                     MDAEGANVRRLTQAGFNTQPRWSPKGDTIVFTSRQGNFDLWGIGPDGSNLRRLTAGPG
                     NNEGASWAPNGRHLVFQSNRLAGGYQLFTMLADGTEQQPLTRAPGESTSASWSPRLP"
     gene            7921..8499
                     /gene="pal"
                     /locus_tag="VFX87_10585"
     CDS             7921..8499
                     /gene="pal"
                     /locus_tag="VFX87_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX4995409.1"
                     /translation="MAHRRGSALLVAPVLVLSLFLAGCPKRPATTAASAPPPTGAPAP
                     SAAAPSTSPGAAMTPSPVAPATAAPSTTPPAPSEFRETENLKDVFFDFDKYDIRAGDA
                     KVLDTNAAWLKTNNSLVLIEGHCDERGTNEYNLALGERRAKATMNYLVGQGVQANRIT
                     IISYGKERPTCTEHSEACWAKNRRSHFLVKAR"
     gene            8571..>8890
                     /locus_tag="VFX87_10590"
     CDS             8571..>8890
                     /locus_tag="VFX87_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995410.1"
                     /translation="MVLAVTLIAAAGCADVSGDALQQDVAQLRQDLNSLTLAVHRGRG
                     DTETVMGQLDRRTREQAAESGKQSAALNARLDALSAEVARVSARVDELSQRVETLGRA
                     APSGG"
BASE COUNT         1356 a         3255 c         3036 g         1243 t
ORIGIN      
        1 gcaggagtcg gtgtccttca agggcgccca ctaccgggtc gagggccgtc tcgagcccaa
       61 gtccgtctcg cggccgcacc cgccggtctg gatcggcggc tggggcgacc tcaccctgcg
      121 gcgcgccgcc accctcgcgg acaactggat cccggggccg accgcggatc tgccgcgcct
      181 gctcgccggc aagcagcagt tcctgaccaa ccgccgggcc gcgggccgcc ccgaggcggt
      241 gaccgaatgg ccgctcaccc gcgacgtgat catcgcggac accgaccggg aggcgcgcga
      301 gctggccgag aggcacatca tgatctcgta ccggaaggag tacgcgggcg gttggcggca
      361 tcccttcatc gacgcctcca tcgccaccga tctcgacggg ctcatgaaga accgcttcct
      421 catcggcggt cccgagcagg tcgtccgcgc gctccggccc ttcgtcgacg agtacggcat
      481 gacccatctc atctgccggg tcttcttccc cggcatgccg caccggcaca tcatgcgcga
      541 gctggagctg ctgtcccggg aagtgctgct cgccttcgcc tgacgcttcg tgccggtgtg
      601 atacgcttcg cccgtgcgct ggctgatcgt cctgccgttc gcccgtcccg ggttgatggg
      661 gatggacttc cgcgacgagc tcgtggccat gggccacgag gtgcggacct tcgcctaccg
      721 gcgcgataac ccgctctaca agaaccgcag caccaaggcc acctaccaga tcttcatcct
      781 ccgccgcctc gagcgcgcct gcctcgcgtg gcggccggac ctcgtgctcg tgatcaaggg
      841 cgggccggtc actcccggcc tcatccggcg cgtcaaggcc cggatcgaca cgcgcttcct
      901 gaacttcttc cccgacaacc cgctgtggat gatcccgttc gactgcgtcg aggcctacga
      961 cgccttcttc accaaggagc gctacgcgct aggggccctc gagcaggcgg gcctgcgcaa
     1021 cctccactac ctgccgatgt actgcgtgcc cgagcagcac cacccgatcg agctgaccgc
     1081 ggaggagacg cggcgctacg cggcgccgat cagcttcgtc ggcaacgcct atccctaccg
     1141 ggcgcgcttc gtgcgcgagc tggcgggcta cccggtgcgc ctctggggcg gaggctggga
     1201 ccgcgtcgag gcgcccgagg tgcgcgcgat ggtggcgggc gggccggtgt gggggcgggc
     1261 caagctcgcg gtgtactcgg gctcgacgct gtcgctgaac caccatcacc cgatgaacga
     1321 catcgtgggc gtgaacacgc gggccttcga gctcgcggcg agcgcggcgt gccaggtggt
     1381 ggacgccaag ggcgagctcg cccacctctt caccgcgggg gaagaggtgg tggtgtacca
     1441 cgacctggcc gagctgcggc accagctcga ccactacctc gcccaccccg acgaggcgcg
     1501 cgacatcggt gagcgggcgc gcaagcgggc gctcgccgag cacaccctgc gccatcgcat
     1561 cgaggcgatg atcgccatgc tcgggcgatg acgcggggag cgatgagacg ggagtcattc
     1621 gccaccgagc tccgctgcat cgcgtgcggg cgcgcgcacc cgctcgacta tgtgctggag
     1681 tgcaccgcct gccgcggtct cctcgagctc gcctacgact gggaggcgct gcgccgcgcg
     1741 gggccgggcg tcttcacggg cggcggcctc tggcgctacg cgccggtgct gcccatcgcc
     1801 gaccccgccc atcgcgtgag cctcggcgag ggccagagcc cgctgctcga ctgcccggcg
     1861 ctcggccgcc ggctcggggt gcgccggctc caggtcaagt tcgagggctc gaaccccacc
     1921 gggaccgtga aggaccgctc ctcccccacc gcggtggcgg ccgcgctcca gttcggcttc
     1981 cgcgccacct cggtggtgag ctccgggaac gcgggctcct ccatcgccgc ttactcggca
     2041 cgcgcgggcc tgcgctcgct gatcttcgcc tacgagcgga cctcggcgcc gaagctcctg
     2101 cacatgaccg ccaccgccac cgatctcgtc ctctaccagg gggtctacga cgacctgatc
     2161 accctgtggg accggctcgt cgaggagcgg ctcttcttcg actgcggcgc ctcgcgcaac
     2221 gcctacaagc acgagggcaa gaagacgctc gcctacgaga tcgcggagca gtcgggctgg
     2281 cgggccccgg acgtggtggt ggcgcccgcg gcggtgggcg agaccttcat cgcgacccac
     2341 cggggcttcg tcgagctcga gcggctcggc tggatcgcgc ggcggcccct gatggtggcc
     2401 gcgcaggcgg cgcgggccaa cgcgatcacg cgcgcgtggc gcggcaagcg acccctcgag
     2461 ccggtgaaga tcgggtacac ggtggccgag ggcctcgccg ccggcaaccc gggccggaag
     2521 ggcgaatggg tcctgcgcat cctgaacgag accggcggcg tggcgggcga ggcggaggac
     2581 gaggagatcc tcgagacgca ggccctgctc gcccgcaccg agggcatctg ggccgggccg
     2641 acgggcgtgg ccgcgctcgc ggtgctgcgg cggctcctcg ccgagaaggc gatcgatccc
     2701 gacgccacca tctgcgtgat cttgagcgag accgggctca agaccgaggc cagcgcgccg
     2761 agccgacggg ccaccgcctt cgacgaggcc tcgctacgcc ggctggtcca ctcgacgctc
     2821 accccctgac cgggcgcagg cgcagtcgtc gcgcggtcag cgccgccatg tgcggatcgc
     2881 gctccacccc gatccactcg cgcccgagcg cggcggcgat gcgccccacc gtgcccgcgc
     2941 ccgcgaacgg atcgagcacg gcgccccggc gggcggagag cgcgagcagc ccgcggagca
     3001 ccggatccgg tgtcgcgtcc cacaggctct ccgggtgcga gcgcggcagc ggcagcatcc
     3061 agcagagcgt ggttccatcc atccgcaccg gatgctccgc cttccggaag cgcagcaggt
     3121 gattcacccg gttgtcccag cgctcgcgcg atcgcacctg cgcccagagc cccttggact
     3181 gcagccgcca gccggcgcgg cgcagatggc tcgtgacgag cgcatccatc cccacccatt
     3241 cggtcccgtc gtggcggtcg ccgatcacga gccagaggtc gccgtggtcg gccagcaccc
     3301 ggcgccactc gcgcgcgaag ccggacgcga gctcgcgcgc gtagcgctcg gcccgcgcgc
     3361 gcccgcgccc gcgcacccag tagggcggcg aggtcaggat caccccgacg ctgccggtgg
     3421 cgatgccggt caggcgccgg ctgtcaccca cgaagagacg gagccgtccg tcgcggctgg
     3481 cgtagcgctt catgccgcgg gggaggatga gccgatcaga tgggacggcg gcgcagcagc
     3541 agcggcacgc ccaccagggc cagggtcatg cagagcaggc tcacgagctg cgcgacgcgg
     3601 agggagccca gcatcagcgg gtcggttcgt aggccttcgg tgaagagccg gccgagcgag
     3661 tagaggccga cgtaggcgag gaacagcgcg cccggcgccc gcgcgagacg atcgcggagc
     3721 acgaacacga ggaggccgaa caccacgaga tcccacacgg actcgtagag gaacgtgggg
     3781 tggaagaact cggcctgggc gaacggcggc ggccggtgcg gcggcgagat gtacagcttc
     3841 cacggcagct cggtgggccg gccgaacgct tcctcgttga agaagttgcc ccatcggccg
     3901 atcgcctgcc cgagcgccag gctgggcgca gccacgtcga gatactgcct gagcggcagg
     3961 cgccgccgcc acgcgtagac cccgcccacc aggatgccgc ccagcacgcc cccgtggata
     4021 gcgagcccgc cttcccagag cgcgaagatt ttaccgggaa actggccgta gtagtcgagg
     4081 ttgaacgcca cgtagtagag ccgcgcgccg atcagaccgc ccagcagggc cagctcggag
     4141 gccttcacca gacgctcgga atcgaggccc cgccggcgcg cgtcccgatg ggcgagccag
     4201 agaccgaggg ccatggccgt cgccatgagg acgccgtacc agcggatcgc gatggggccc
     4261 acgtgcaccg cgatggcgcc cggcgactgg aacatagaag gcttatacgg ccggaccccg
     4321 agactgtcaa taattcagcg acttttcagg cattttccgg gcgaatttgt tgacaccctg
     4381 aaaacggctc cggtatcatc aaatcaaaca attcgtcacc atcgttcacc attcacccgt
     4441 actaaggaga ccgccgggtg ccagggtcag gaagtgccaa cggcggcgtt gcggagctcg
     4501 tcctcagcgc cggtcccgtc gccaagttcg tcctgctcct cctcgcgacc ttttccgtca
     4561 tctgctgggc gctgatcgtc gagaagtggt gggagttccg gaagatccgg cgcgagtcgc
     4621 tccggttccg gcagatcttc cgggaagcgc gccgcttctc ggtggtctac ggggcggcca
     4681 agaagcaccg cgacagcccg ctggcccagc tctacgtggc ggcgggccag gagctggtca
     4741 ccgcctacgg cagcgcggag atggtggacc acgtgctgga cgacgcggac gaagggctgg
     4801 ccgtcgagcg cctggacgcg accagccgcg cgctccggcg cgccggctcc gccgaggtcg
     4861 cgcgcatgga gcgctacctg cccttcctcg ccaccgtggc cagctcggcg ccgttcatcg
     4921 ggctgttcgg cacggtgtgg ggcatcatga ccgccttcca gaacatcggc cagcagggct
     4981 cggccaacct cgcggtggtg gcccccggca tctcggaagc gctcgtggcc accgcggccg
     5041 gcctgggcgc ggccattccc gcggtcatgg gctacaactt cttcgtgaac cgcaccaagc
     5101 actgggcgat ggagatggaa ggcttcacgc tggacctgct gaacgccttc gctcgtccgg
     5161 cgcagaagat ggcacgggtg cccaaagatg gccattaagg tcgacacgcc cgactacggc
     5221 agcggcggca gctatcgcat cggcgggacc atgtccgaga tcaacatcat cccgctggtc
     5281 gacgtcatcc tcgtgctcct cctcatcttc atgctcaccg cgccgctcat gtaccgcggc
     5341 atcgacgtca acctgccgaa gagctcgggc aagcccaccg cggtcgagga gcgggtggtg
     5401 ttgacggtga cgaaggagca gaccatctac atcaacgaca agccggtggc cctgggagcg
     5461 gtcgagcagg cgctgcgcga cctgttcaag aaccggcagg acaagacact ctacctgcgg
     5521 gcggaccagg ccctgcagta cggcctcgtg gtcgagacca tggatcgcgt gcggcgcgcc
     5581 gggatcgaga agctgggcat ggtgacggag ccgacccgcg ctcgatgagc tccacgatgg
     5641 ccgcccgccg ctaccccatc ggcccgcttc ccgatcccgg tctgagacga ccgggtccgg
     5701 cgctgcggcg gcggcgcctg cccctggcgg ccatcggctt ctccaccgtg gggcacgtcg
     5761 tggtgttcgg cgggatggct gcgttcgtga tgtggggcgg ctggcgtccc gagaagatcc
     5821 aggtcgtcaa cctggtgccg atggtggccg cggtcggcaa cccgaacgcc ccggctgccc
     5881 cgaccctgcc cgcgcgcccg caggcgccgg tggcgccgcg cgcgccggag cccgagccca
     5941 aggccaccac cgccccgcgt gaagcgccga agcgcgaggc gccgagcctt cccgcgccgt
     6001 ccttgcccgc ggcccggccg ctgccgccgc gggccaccct gccgcgccca ggggagaagg
     6061 agctgccgtc gctggcgacg ccgccggccg gcaccccgcg gggcgagaag accgcggagt
     6121 ccgcgcggcc gcccgagacc gcgtcgctcg gcgcgccgac cggctccgtg accggcgcgg
     6181 gcgccctgac cctcgatgtg ggcgacttcc cgcacgcgtg gtacctccgg caggtgctcc
     6241 agaaggtcga gcaggagtgg cagcggcaga accagcccag cgacccgcag cagaagccgc
     6301 agatctacgt cgagatcctt cgggacggct cgatcaagcc gcccgccatc cacaaaagct
     6361 cgggcaacgc cttctacgac aacgcggccc tccgcgcggt catggcggcg ggcccttttc
     6421 cgaaactgcc cgacgactgg gcgaagccgt cgcttcgcat cctcttcacc ttcgacattc
     6481 gtcgcggctg acacatgaat gggtgaatca tgaacaagat gatgcgtccc ggacgtccgc
     6541 tcggcttcct cgtcgggctg gtgctgctgg ccgcgctggg cggcgcgctg gcctcgccgc
     6601 ccgccgcccg cccgcaggcc gccgacgtgc tgctcaacgt gctggccacc ggagccaaga
     6661 agctcaacat cgtgatcccg cacttcacgg tcgtgggcgg ccccgatccc cagggcctcg
     6721 ggcgtagcct gtcccaggtg accgcgcgtg atctgacatt ctccagcctc ttcagcgtcg
     6781 tctcggacgt gcccgcgatc cccgtcgggg atcccgccgc actccgggcc gccttcgcca
     6841 acttcgcggc ggcgggcgcc cacgcgggcc tgcagggcgt gctcaccctg cgcggcgacc
     6901 gcgccgagat cgagatgcgg ctgcacgacc tgacctcgcc cgagttccgg ctgatcggcg
     6961 ccaagaagat cgagctgccc accgcgcagg cgcgccgact ggcccacaag gtggcggacg
     7021 aggtggttct gcagttcacc ggcgaggcgg gcgtcgcgga caccaaggtc gcctacgtgg
     7081 cctcgggctc cggcagcaag gagctcagcc tgatggacta cgacggcgcg agcgcggcgc
     7141 gcgtgacgtc gaacaagtcc atcaatatct ccccggtgtg gagcccggac acccgctcgc
     7201 tggccttcac atcctacatg cggggctacc cgtacctcta ccggatgttc ccgttcgaga
     7261 accggccggt gcaggtgctg gccggcttcc tcggcatcaa tacctcgccc gccttcagcc
     7321 cggacgggcg ctcggtcgct ttgaccctct cccgtgacgg caatcccgag gtgtacgtgc
     7381 tgaacctcgc ctccggggcc tttcgccggc tgaccaccta cagcggcatc gacaccgagc
     7441 ccagctggtc tccgaccggg cgggagatcg ccttcgtgtc cgagcgggcc ggaggggctc
     7501 acatcttcgt gatggacgcg gagggtgcga acgtgcggcg cctcacccag gccgggttca
     7561 acacgcaacc ccgctggtcc cccaagggcg acaccatcgt cttcacgtcg cgccagggca
     7621 acttcgatct ctggggcatc ggccccgacg ggtcgaacct gcgccggctc accgccggcc
     7681 ccggcaacaa cgagggcgcc tcctgggccc cgaacggccg ccatctcgtc ttccagtcca
     7741 accggctggc gggcgggtat cagctcttca ccatgctcgc cgacggcacg gagcagcagc
     7801 ccctcacgcg ggcccccggg gagtccacaa gtgcttcttg gtcaccgcgc cttccgtgat
     7861 acgatatttc ggtggtgaag aatgatcatc aagcgcatga aacctaggag gatttgactg
     7921 atggcgcatc gacggggaag cgcgctgctg gttgctccag tgctcgttct ttctctcttc
     7981 ctcgcggggt gcccgaagcg gcccgccacc acggccgcct ccgcgcctcc gccgaccggc
     8041 gcgcccgcgc cctcggccgc ggcgccctcc acgtcgcccg gcgccgcgat gacgccgtcg
     8101 cccgtcgcgc ccgcgaccgc tgcgcccagc acgacgccgc ccgcgccgag cgagttccgg
     8161 gagacggaaa acctgaagga cgtcttcttc gacttcgaca agtacgacat ccgggccggc
     8221 gacgccaagg tcctcgatac gaacgcggcg tggctgaaga ccaacaacag tctcgtgctg
     8281 atcgaaggcc actgcgacga gcgcggcacc aacgagtaca acctggcgct cggcgagcgg
     8341 cgcgcgaagg cgacgatgaa ctacttggtc ggccagggcg tacaggccaa ccgcatcacg
     8401 atcatctcct acggcaagga gcggcccacc tgcaccgagc acagcgaagc gtgctgggcc
     8461 aagaaccggc ggtcgcactt cctcgtgaag gcccgctgat cgacccggag ttcgccgtgt
     8521 cgcgccgaac gaccgcgaag gggagcctcc ggctcccctt cgcggcttgc atggtcctcg
     8581 ccgtcacgct catcgccgcc gcgggctgcg ccgacgtctc cggcgatgcg ctgcagcagg
     8641 acgtggccca gctccgccag gatctcaact cgctgacgct ggcggtgcat cgcggccgcg
     8701 gggacaccga gaccgtgatg ggccagctcg atcgccgcac ccgcgagcag gccgcggaga
     8761 gcgggaagca gagcgcggcg ctcaacgcgc ggctcgacgc gctctccgcg gaggtcgccc
     8821 gcgtctccgc ccgcgtggac gagctgtccc agcgagtgga gaccctcggg cgcgcggcgc
     8881 cttcgggcgg
//
LOCUS       DASUFA010000282         5815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1107689, whole genome shotgun sequence.
ACCESSION   DASUFA010000282 DASUFA010000000
VERSION     DASUFA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107689"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VFX87_10595"
     CDS             <1..1267
                     /locus_tag="VFX87_10595"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX4995411.1"
                     /translation="REHTIAREMTHLYFTGFDCLGCRAAYAPDQDLLLCPRCHQLLEA
                     RYDLARFGREMDRDALAGRPAGVWRWRELLPVLDPSRIVTLGEGGTPFLRSDRIAQAC
                     GVRELWLKSDAANPTGSLKDRSITVSATKAMEFGYDVLACDSTGNKASSTAAYAARAG
                     LRSVVFCPKDTPVPKMAQAVFFGARLIRVDGHYSQINAMYRKLIGSGRVKWYDCGTDN
                     PFRYEGKKTYAYEIAQDLGWQVPDRVLHPCAGGMSLVKTWKGFSELRTLGWTDRLPKM
                     TAVQAARCAPIVESWKKGLPRVEPVDKQPTIASAVAAADPGLLGDQTLLAVRASDGDA
                     LAVEEAEIVEAVRLLAQEGLMIEPSGAVTVAAVRRGVRDGLIDRGESVVCVLTGSGFK
                     DFDRILDLVDIPRNVVSGYEAMLAEAERI"
     gene            1272..1742
                     /locus_tag="VFX87_10600"
     CDS             1272..1742
                     /locus_tag="VFX87_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4995412.1"
                     /translation="MPDARRIEKKLAALGLELPSPPVPVGNYLPAARVGNLVFTSGQT
                     ARINGVRRYVGVVGQEVSQADAYLSARDATLNCLACVKQTVGSLDRVKRVVKVMGFVN
                     AGREFDHQPAAINGASDLLEQLFGEAGRHARSAIGLSSLPSNVSVEIELIVEVR"
     gene            1743..2828
                     /locus_tag="VFX87_10605"
     CDS             1743..2828
                     /locus_tag="VFX87_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4995413.1"
                     /translation="MRAAVMRNKRLVVADLPQPDPGPGEVLVKTLACGICGSDLHALR
                     HAEQFVAASRRAGSVFTMDLTRDVVMGHEFCAEIVDYGPATTRALRVGARVCSRPSLV
                     RADGPRTVGYSNDTPGGYAEYMRLTESLLLPVPDGLVTEHATLTEPMAVGLHAVARAR
                     LEPDDAPLVIGCGPVGLAVIAALRLHGARPIVAADFSARRRELATALGADLVVDPATT
                     TPWQSWREAAVWPDASRAPLLPPWIKGPAVRPAVVFECVGVPGVLDQIMAASPRGTRI
                     VVVGVCMEADTIYPMLGISKELNLQFVLGYTPDEFAATLNHIAEGRIPVAPLITGKVG
                     VEGVAGAFEALASPERHAKILVEPWRA"
     gene            2825..4024
                     /locus_tag="VFX87_10610"
     CDS             2825..4024
                     /locus_tag="VFX87_10610"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995414.1"
                     /translation="MTTFAALRERNYRFYWVGLVFYVLGHRAEYVTFAWLVWEATHDA
                     LHLGYLGLAQGVPLVVFQLWGGVLADRVNRLRLLLATQVLTTVTLLAAFALSALGAIR
                     VEHLLALAALSNTFRAFDEPTRMSMVPQMVGRERLANAIALGSIPWQAGRMIGPSITG
                     ILIAAFGGTVGLAIAAVSSGMALTMYARLRVDARSAAAPAGHMGTQLVEGLSFVRHNF
                     VFAALISLALFNAVFGLSYVTLLPIYADVYFQAGSTGFGLLNAAHGTGALVGTLIVAY
                     LAARFRRRGLIILVGAAGMGLGLAIFSQSPGLGLALPILALVGFSNTFYLTQVSTHLQ
                     QAVPDHLRGRVMSLYALCWNLLPLGGLLGGVLASAVNARFAVLVGGAMVAANALVLLA
                     SRRLRLL"
     gene            4039..5286
                     /locus_tag="VFX87_10615"
     CDS             4039..5286
                     /locus_tag="VFX87_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995415.1"
                     /translation="MSSSRVFYGWWVTVAFSVMVFVSAGVRHAVGPFLKPMAADLGVD
                     RGSFSLVIALGLFLYGAFMPWIGGLTDRFGARRITAAGSVLLAVSLVLTAYCHNLWQL
                     ALVYGVLAALGLACTGPVVANAVVTRWFVRRRGTAVSLLGSAAMTGMSLLVPVVAWLI
                     VVFDWRTAYMVLGAAVLVGMLPLCLFVVRDSPEEMGLRPDGDTAVAGAPAPVITRTPA
                     GTALRTAAFWQLGGSFFTCGFSMALVAAHGVPMLTDHGYTPMFASWVIGVLGASSIVF
                     TLTLGRLSDQFGARPVLAGIYAGRALIFAGLFLIRDNPAAMIGVAIVGGITLAGSMSM
                     TSVLTADIYGRYSVGSILGVIFLVHQTGAALGSWLAGALFEATGGYGAMFAIAAILLA
                     VAGLVSLHVDREPRMLRWATLTR"
     gene            5304..5612
                     /locus_tag="VFX87_10620"
     CDS             5304..5612
                     /locus_tag="VFX87_10620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX4995416.1"
                     /translation="MADVLTVIAKIRAVKGKGDALAALLKEQVAVVTKAEPGLLIYRP
                     HRSSKDPDLFVFYEQYKDDAAFELHRKAPYLAAYRERREKEGLTEGPAEVEIYRSLTD
                     "
     gene            complement(5609..>5815)
                     /locus_tag="VFX87_10625"
     CDS             complement(5609..>5815)
                     /locus_tag="VFX87_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995417.1"
                     /translation="VTTETGWRVPEPLQARLFDGHAIEVPIFRWPAPPRRHVRISAQL
                     YNSAAQFVRLADALRKELAAERTR"
BASE COUNT          714 a         2146 c         2026 g          929 t
ORIGIN      
        1 ccgggagcat actatcgccc gggagatgac gcacctgtac ttcaccggct tcgactgcct
       61 cgggtgccgc gccgcgtacg cgccggacca ggatctcctg ctctgcccgc gctgccacca
      121 gctcctcgag gcccgctacg atctggcccg cttcggccgc gagatggacc gcgacgcact
      181 ggccgggcgc cccgccggcg tctggcgctg gcgggagctg ctgcccgtgc tggacccgtc
      241 gcgcatcgtg accctcggcg agggcggcac cccgttcctg cgctcggacc ggatcgccca
      301 ggcctgtggc gtccgcgagc tgtggctgaa gtccgacgcg gccaatccca ccggctcgct
      361 caaggaccgc tcgatcacgg tgtcggcgac gaaggcgatg gagttcggct acgacgtgct
      421 ggcctgcgac tccaccggca acaaggcgtc gtccaccgcg gcctatgccg cgcgcgccgg
      481 cctccgcagc gtggtgttct gcccgaagga cacgccggta ccgaagatgg cgcaggcggt
      541 cttcttcggc gcccggctca tccgcgtgga cggccactac tcgcagatca acgccatgta
      601 ccggaagctg atcggcagcg ggcgcgtcaa gtggtacgac tgcggcaccg acaacccgtt
      661 ccgctacgag ggcaagaaga cctacgcgta cgagatcgcc caggacctcg gctggcaggt
      721 cccggaccgg gtgctgcatc cgtgtgccgg gggcatgtcg ctcgtgaaga cgtggaaggg
      781 cttcagcgag ctccgcacgc tcggctggac cgaccggctg cccaagatga ccgcggtgca
      841 ggccgcccgc tgcgcgccca tcgtggagtc gtggaagaag ggcctcccgc gcgtggagcc
      901 ggtggacaag cagcccacga tcgccagcgc ggtggccgcg gccgacccgg ggctcctggg
      961 cgaccagacg ctcctggccg tccgcgcctc cgacggcgac gcgctcgcgg tggaagaggc
     1021 cgagatcgtc gaggcggtgc ggctgctcgc ccaggagggg ctcatgatcg agcccagcgg
     1081 cgcggtcacc gtcgcggcgg tgcgccgggg cgtgcgggac ggcctcatcg accgcggcga
     1141 atcggtcgtc tgcgtgctca ccggctccgg cttcaaggac ttcgaccgca tcctcgactt
     1201 ggtcgacatc cctcgcaatg tcgtgtcggg ctacgaggcc atgctggccg aagccgagcg
     1261 gatctgacac ggtgccggac gcgaggcgga tcgagaagaa gctcgccgcg ctggggctcg
     1321 agctgccttc gccaccggta ccggtgggca actatctccc cgccgcgcgc gtggggaatc
     1381 tcgtcttcac ctccgggcag accgcccgga tcaacggggt gcgccgctac gtgggcgtgg
     1441 tcggccagga ggtgagccag gcggacgcct acctctcggc gcgcgacgcg acgctcaact
     1501 gcctggcgtg cgtgaagcag acggtcggct cgctcgaccg ggtgaagcgc gtggtcaagg
     1561 tcatgggctt cgtgaacgcg gggcgcgagt tcgaccacca gcccgcggcc atcaacggcg
     1621 cgtcggacct gctcgagcag ctcttcggcg aggccgggcg ccacgcccgc agcgcgatcg
     1681 ggctgtcctc cctgccgtcc aacgtctcgg tcgagatcga gctgatcgtg gaggtgcgat
     1741 agatgcgcgc agccgtgatg cggaacaagc gactcgtggt cgccgacctg ccccagcccg
     1801 atccggggcc gggcgaggtg ctggtcaaga ccctggcctg cgggatctgc ggctccgatc
     1861 tccacgcgct ccgtcacgcc gagcagttcg tggcggcctc gcgccgcgcg ggcagcgtct
     1921 tcaccatgga cctcacgcgc gacgtggtga tgggccacga gttctgcgcc gagatcgtgg
     1981 actacgggcc cgcgacgacg cgggcgctgc gggtcggcgc gcgcgtctgc tcccggccga
     2041 gcctggtccg cgccgacggc ccgcgcaccg tgggctactc caacgacacg cccggcggct
     2101 acgccgagta catgcggctg accgagtcgc tgctgctccc ggtgccggac ggactcgtca
     2161 ccgagcacgc cacgctcacc gagccgatgg cggtaggcct ccacgcggtg gccagggcgc
     2221 gcctcgagcc cgacgacgcc ccgctcgtga tcggctgcgg cccggtgggc ctggccgtga
     2281 tcgcggcgct gcgcctgcac ggcgcgcggc ccatcgtggc cgcggacttc tcggcccgtc
     2341 gccgcgagct ggccaccgcg ctgggcgccg acctggtggt ggatcctgcc acgaccacgc
     2401 cgtggcagag ctggcgcgag gccgcggtct ggcccgacgc gagccgcgcc ccgctcctgc
     2461 cgccctggat caagggcccc gcggtgcgcc cggcggtggt cttcgagtgc gtgggcgtgc
     2521 ccggcgtgct cgaccagatc atggccgcct ccccgcgggg cacgcgcatc gtcgtggtcg
     2581 gcgtgtgcat ggaagccgac acgatctacc cgatgctcgg catcagcaag gagctgaacc
     2641 tgcagttcgt gctgggctac acgccggacg agttcgccgc cacgctcaac cacatcgccg
     2701 agggccgcat cccggtcgcg ccgctgatca ccggcaaggt gggtgtcgag ggcgtggccg
     2761 gcgcgttcga ggccctcgcc tcgcccgaac ggcacgccaa gatcctcgtc gagccctggc
     2821 gggcttgacg accttcgcgg cgctccggga gcgcaactac cgcttctact gggtcgggct
     2881 cgtgttctac gtgctcggac accgcgccga gtacgtgacc ttcgcctggc tggtctggga
     2941 agcgacccac gacgcgctcc acctgggcta tctcgggctc gcccagggcg tcccgctcgt
     3001 cgtcttccag ctctggggcg gcgtgctcgc cgatcgggtg aaccggctgc gcctgctgct
     3061 cgccacgcag gtgctcacga ccgtgaccct gctggccgcc ttcgcgctct ccgccctggg
     3121 agcgatccgc gtcgagcacc tcctggccct ggccgcgctc agcaatacct ttcgcgcgtt
     3181 cgacgagccc acccgcatgt ccatggtgcc gcagatggtc gggcgcgagc ggctcgccaa
     3241 cgcgatcgcg ctgggctcga tcccgtggca ggccggccgc atgatcggcc cctccatcac
     3301 cggcatcctg atcgcggcct tcggcggcac ggtcggcctc gccatcgccg cggtctcctc
     3361 cgggatggcg ctgacgatgt acgcgcgcct gcgcgtggac gcacggtccg ccgcggcgcc
     3421 cgccgggcac atgggcaccc agctcgtcga ggggctgagc ttcgttcgcc acaacttcgt
     3481 gttcgcggcg ctgatcagcc tggccctctt caacgcggtc ttcggcctct cctatgtgac
     3541 gctgctgccg atctacgccg acgtgtactt ccaggcgggc tccaccggct tcggcctgct
     3601 caacgccgcg cacggcaccg gcgcgctcgt gggcaccctg atcgtggcct acctggccgc
     3661 gcgctttcgc cggcgcggcc tcatcatcct ggtcggcgcg gcgggcatgg gcctggggct
     3721 cgcgatcttc tcgcagtctc ccggcctcgg cctggccctg ccgatcctcg ccctggtcgg
     3781 cttcagcaac acgttctatc tcacgcaggt gagcacgcat ctccagcagg cggtgcccga
     3841 tcacctgcgc ggccgcgtga tgagcctcta cgcgctctgc tggaacctgc tgccgctcgg
     3901 cggcctgctg ggcggcgtgc tcgcgtccgc ggtgaacgcc cgcttcgcgg tgctggtggg
     3961 cggcgcgatg gtcgcggcca acgcgctcgt cctcctcgcg tcccgccggc tccggctgct
     4021 ataatcgcgg gcaccttcat gtcctcgtcc cgggtgttct acggctggtg ggtcaccgtc
     4081 gccttctccg tcatggtctt cgtctcggcc ggcgtgcgtc acgcggtcgg ccccttcctc
     4141 aagccgatgg ccgccgacct gggcgtggac cgcggctcct tctcgctggt gatcgcgctc
     4201 ggcctcttcc tctacggcgc cttcatgccg tggatcggcg gcctcaccga ccgcttcggc
     4261 gcgcggcgga tcaccgcggc gggctcggtg ctcctggccg tctccctcgt gctgaccgcc
     4321 tactgccaca acctctggca gctcgccctg gtctacggcg tgctcgccgc gctcggcctc
     4381 gcctgcaccg gcccggtggt ggccaacgcg gtggtcacgc gctggttcgt gcgccgacgc
     4441 ggcaccgcgg tgtccctgct cggcagcgcg gcgatgaccg gcatgagcct gctggtgccg
     4501 gtggtggcct ggctcatcgt ggtgttcgac tggcggaccg cctacatggt cctgggcgcc
     4561 gcggtgctgg tgggcatgct gccgctctgc ctcttcgtgg tgcgcgactc gcccgaagag
     4621 atggggctgc ggcccgacgg cgacacggcg gtggcgggcg cccccgcccc ggtcatcacc
     4681 cgcacccctg ccgggaccgc gctgcgcacg gcggccttct ggcagctcgg cggctcgttc
     4741 ttcacgtgcg gattctcgat ggccctggtg gccgcgcacg gcgtgccgat gctcaccgac
     4801 cacggctaca cgccgatgtt cgcctcgtgg gtgatcggcg tgctcggcgc ctcgagcatc
     4861 gtcttcacgc tgacgctcgg ccggctgtcg gatcagttcg gcgcgcggcc ggtgctggcc
     4921 ggcatctacg cggggcgcgc gctcatcttc gccgggctct tcctcatccg ggacaacccg
     4981 gccgcgatga tcggggtggc catcgtgggc ggcatcaccc tggccggctc catgtccatg
     5041 acctcggtgc tcaccgccga catctacggc cgctactcgg tcggctccat cctgggcgtg
     5101 atcttcctcg tgcaccagac cggcgccgcc ctcggctcct ggctcgcggg cgccctcttc
     5161 gaggcgaccg gtggctacgg tgccatgttc gccattgccg ccatcctgct ggccgtcgcc
     5221 ggcctcgtca gcctgcacgt ggaccgggag ccgcgcatgc tccgctgggc caccctcacc
     5281 cgctagaacc aaggagatca gtcatggccg acgtgctcac ggtgatcgcg aagatccggg
     5341 cggtgaaggg caagggcgac gcgctggccg cgctgctcaa ggaacaggtg gccgtcgtga
     5401 cgaaggcgga gccgggcctc ctgatctacc gcccgcatcg ctccagcaag gaccccgacc
     5461 tcttcgtctt ctacgagcag tacaaggacg acgccgcgtt cgagctgcat cgcaaggcgc
     5521 cgtacctggc cgcctaccgg gagcgccgcg aaaaggaagg cctgaccgag ggccccgccg
     5581 aggtcgagat ctaccgctcg ctgacggact agcgggtccg ctcggccgcg agctccttgc
     5641 ggagcgcgtc ggccagccgg acgaactgcg cggcgctgtt gtagagctgg gcagagatgc
     5701 ggacgtggcg ccgcggcggc gcgggccagc ggaagatcgg cacctcgatg gcgtggccgt
     5761 cgaagagccg cgcctggagc ggctcgggca cccgccaccc ggtctcggtc gtcac
//
LOCUS       DASUFA010000283         7035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1199175, whole genome shotgun sequence.
ACCESSION   DASUFA010000283 DASUFA010000000
VERSION     DASUFA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199175"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="rpsB"
                     /locus_tag="VFX87_10630"
     CDS             complement(<1..722)
                     /gene="rpsB"
                     /locus_tag="VFX87_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX4995418.1"
                     /translation="MAALTMKELLEAGVHFGHQTKRWNPKMQKYIFGERNGIYIIDLQ
                     KTLKKFREAYAFVRDLAADGGSVLFVGTKKQAQETVLEETTRCGQFYVNHRWLGGTLT
                     NFTTIRKSIARLKKLDEMSESGEYERLPKKEVIGLERERAKLQSTLVGIKNMDRLPSA
                     VFIIDPKKETIAVEEARRLSIPIVAIVDTNCDPSGIDYPVPGNDDAIRSVRLITSRIA
                     DAIIEGSGSLAKQEAEEEIPASP"
     gene            complement(852..2066)
                     /gene="argJ"
                     /locus_tag="VFX87_10635"
     CDS             complement(852..2066)
                     /gene="argJ"
                     /locus_tag="VFX87_10635"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX4995419.1"
                     /translation="MPDFQWLEGGITAVPGILASGVAAGIKPSGKKDLALVYSSSPAR
                     AAAVFTTNQIKGAPVLLSQEHVRDGRAQAILASAGCSNVCTGEQGMKDAREMTRITAD
                     LLRIPPRQVLIASTGVIGQPLPMDKIRAGLPKLVKGLSPQGGRLAAEAIMTTDTRPKE
                     AALRFDLAGRPVTIGGIAKGVGMLEPHMATMFCFLATDAMVARDSLPRVLRRATDRSF
                     NRITVDSDQSTSDTVAILANGLAENAPLERGGKGLRQFARALEAITERLARMLVEDGE
                     GASKLVEVAVRGARTRREAVIAARSVANSPLVKTAVNGADPNWGRILMALGKSAARVA
                     ADRVALTIGGEPLVEKGMLRPGARIDRIREVMGGPSYTIAIDLGLGRGEDRVWTSDLS
                     EEYVRINAKYTT"
     gene            complement(2072..3121)
                     /gene="argC"
                     /locus_tag="VFX87_10640"
     CDS             complement(2072..3121)
                     /gene="argC"
                     /locus_tag="VFX87_10640"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX4995420.1"
                     /translation="MLKVAVTGASGYMGAELLRLLSVHPKVRISAVTSERLAGERLDR
                     LYPHLRGLSDLVFQEIDGERLSALADVVFLALPHMESQRLMPTLRRHGKKAVDLSADY
                     RLKDASLYTTWYKAPHADASGLTEAVYGLPELHRKQVAGASLVASPGCYPMGAILATA
                     PLLKSGRGSVDGIVIDGKSGVTGAGAQGRKADAMYLFTEANENVQAYGIATHRHTPEI
                     EQELSLLAGRPVVVAFTPHLVPLNRGLFTTASVPLTKPATTAELVALYQEFYAGEPFV
                     RVLDEGQRPTTRSVVGSNFCDVTVVADPRTKRAVCVSALDNLGKGGSANGIQNLNIMM
                     GWDERTGLDAPPVYP"
     gene            complement(3139..3534)
                     /gene="rpsI"
                     /locus_tag="VFX87_10645"
     CDS             complement(3139..3534)
                     /gene="rpsI"
                     /locus_tag="VFX87_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX4995421.1"
                     /translation="MAVVSRFYGTGRRKTSVARVWLRAGSGRILVNRRPFEDYFPRET
                     LRMVISQPLQLTNTFGQFDAIVNVGGGGPTGQAGAVRHGIARALLQFDDKLRQTLKRA
                     GLLTRDPRMKERKKYGQPGARSKFQYSKR"
     gene            complement(3543..3986)
                     /gene="rplM"
                     /locus_tag="VFX87_10650"
     CDS             complement(3543..3986)
                     /gene="rplM"
                     /locus_tag="VFX87_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX4995422.1"
                     /translation="MTTLMPKEEDVERKWFVVDAQDRVLGRLATQVATVLRGKHKPQF
                     SPHLDVGDHVVVINAEKVHLTGRKLTDKIYRWHSGYIGGLREVSAERMLKTHPERVIE
                     WAVQGMLPKGRLGRAMAKKLKVYKGADHPHAAQKPEPLAMRERAR"
     gene            complement(4078..4767)
                     /gene="nth"
                     /locus_tag="VFX87_10655"
     CDS             complement(4078..4767)
                     /gene="nth"
                     /locus_tag="VFX87_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX4995423.1"
                     /translation="MAESLADKKARAGKILTRLAKAYPDARTALDFTTPLELLVATIL
                     SAQCTDERVNAVTAALFPRYRRAADWAGVPLPVLEREIHSTGFFRAKAKSLNGMARAL
                     VERHGGLVPRTLEELVELPGVGRKTANVVLGNAFGIPALAVDTHVFRVSQRLGLARGD
                     DAEKVHDQLCQVLPRPRWTQASHLLILHGRRVCTARKPACPTCPILALCPWPGKTKAA
                     PAGPGARRRAG"
     gene            complement(4767..6965)
                     /locus_tag="VFX87_10660"
     CDS             complement(4767..6965)
                     /locus_tag="VFX87_10660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4995424.1"
                     /translation="MTGRHRLALAAVLIGLAAIMVVWLGIDRRPPEWDHANHLERAVA
                     CHRSLGESGGDWLQEIIRESSFYPPIVTCAAGLLYFALPIVPLTAQAVIWAFLVVGTL
                     AVFGLGRRLLDADAGLLAAFLFATAPFVVFMLLNFQLDLPLAAVVALALYVLVRTEDL
                     AVRPWVLALGLVLGLGMLTKPPFAAYLSGPLLWGAWRGLRAPDRRARLLHLLAAVGTA
                     AAVALPWYGPRLVGLPAQFINRSFKQAAEAGQAPALSGASLLFYPEWIIPQWGLLATA
                     LLLWGIVALARRPQARGLLWAALAPFLIFVLIQNKNLRYTLPLLPAAALVAAAGVSVL
                     PPRVRRGLSWACAIMGVAQVGSAAFALPPPTLLAPVVFSMAFSSPPDGRDWHHREILE
                     AVLRESGPREARVAVVPNDNFFSVSNFRYEVTRDRLPLRLSRAWEGAPLGVDFAIVKT
                     GDQGPDATSAKPDRIMAALGGGDPWLAAAYPVIARVPLPDGSEGMVRMRRPEPVRGVA
                     PAELAERLRRGVAGLLADVVRDAKGLRVTLDYRDEALAAGSVDGVTVEAQSALVGEFA
                     RRKPPLRIGPVRLRVRGLVVNPGRLAATGAVELLDIGSLHVERLGVAERDLAEFIAQQ
                     RGLAGLRATMDEGWIQVRWALPGPGLSARLALEAGTPPDPFTLRAADVRYGGVRLPRL
                     LVDWIVRNFDPSPRLRRLPIPVSMGPIHIRRERLEVGEDGAETAATKESR"
BASE COUNT         1031 a         2518 c         2461 g         1025 t
ORIGIN      
        1 ggcgaggcgg ggatctcctc ctcggcctcc tgcttggcga ggctgccgct gccctcgatg
       61 atcgcgtccg cgatccggga ggtgatgaga cgcacggagc ggatcgcgtc gtcgttaccg
      121 ggcaccgggt agtcgatgcc ggagggatcg cagttggtgt ccacgatcgc cacgatgggg
      181 atggagagcc ggcgggcctc ctccactgcg atcgtctcct tcttgggatc gatgatgaag
      241 accgcggagg gcagccggtc catgttcttg atgccgacca gcgtgctctg cagcttcgcc
      301 cgctcgcgct cgagcccgat cacttccttc ttgggcagcc gctcgtactc cccgctctcg
      361 ctcatctcgt cgagcttctt gaggcgggcg atggacttcc ggatcgtggt gaagttggtg
      421 agggtgccgc ccagccagcg gtggttgacg tagaactgcc cgcagcgggt cgtctcctcc
      481 agcaccgtct cctgggcctg cttcttggtc cccacgaaga gcacgctgcc cccgtcggcg
      541 gcgagatcgc gcacgaacgc gtaggcctcg cggaacttct tgagagtttt ctgcaggtcg
      601 atgatgtaga tgccgttgcg ctcgccgaag atgtacttct gcatcttcgg gttccagcgc
      661 ttggtctggt ggccgaagtg caccccggcc tccagcaact ccttcatggt caatgctgcc
      721 atggtcctgc cgtcctttcc gtcgtcgttc ggttggcctc cggtcgcgag cggccggccc
      781 ccggagaggg gcgcgacact tccccgctag ccgacgaggg cggcggagac cgaagctcgg
      841 aaccgtgcgt gttaggtcgt gtacttcgcg ttgattcgaa cgtactcctc actcaggtcc
      901 gatgtccaca cccggtcctc gccgcgcccc agccccaggt cgatggcgat ggtgtagctc
      961 ggtccaccca tcacctcacg gatccggtcg atccgggcgc ccggccgcag catccccttc
     1021 tccaccaggg gctccccgcc gatcgtgagg gccacgcgat cggcggccac ccgcgccgcg
     1081 ctcttgccca gcgccatcag gatacgcccc cagttggggt ccgccccgtt gacggcggtc
     1141 ttcaccagcg gggagttggc cacggaccgg gccgcgatca ccgcctcgcg gcgggtgcgg
     1201 gctccccgca ccgccacctc gaccagcttg ctcgcgccct cgccgtcctc gaccagcatg
     1261 cgggcgagcc gctcggtgat cgcctccagc gcccgcgcga actgccggag ccccttgcct
     1321 ccgcgctcga gcggcgcgtt ctcggccagc ccgttggcca ggatcgccac cgtgtcgctg
     1381 gtggactgat cgctgtccac ggtgatccgg ttgaaggagc ggtcggtggc ccggcgcagc
     1441 acgcgcggta gcgagtcacg cgccaccatc gcgtcggtgg cgaggaagca gaacatggtc
     1501 gccatgtggg gctcgagcat gcccaccccc ttggcgatgc cgccgatcgt gaccggtcgc
     1561 ccggccaggt cgaagcggag ggccgcttcc ttcgggcggg tgtcggtggt catgatggcc
     1621 tcggccgcca gccggccgcc ctgcggcgag agccccttga ccagcttggg cagccccgcc
     1681 cgaatcttgt ccatcgggag cggctggccg atcacgccgg tcgaggcgat cagcacctgc
     1741 cgcggcggga tccgcagcag gtcggcggtg atgcgcgtca tctcgcgcgc gtccttcatc
     1801 ccctgctcgc cggtgcacac gttggagcag cccgccgagg ccaggatggc ctgggcgcgg
     1861 ccgtcacgca cgtgctcctg tgagaggagt accggcgcgc ccttgatctg gttcgtggtg
     1921 aacaccgcgg ccgcgcgggc gggcgacgag gaatagacga gcgccaggtc cttcttgccg
     1981 ctgggtttga tgcccgcggc aacgcccgag gcgaggatgc cgggcacggc ggtgatgcct
     2041 ccctccagcc actggaagtc cggcatgccg gctacggata caccggaggc gcgtcgagac
     2101 cggtccgctc gtcccacccc atcatgatgt tgaggttctg gatcccgttg gcggagccgc
     2161 ccttgcccag gttgtcgagc gcggacacgc agaccgcgcg cttggtgcgc gggtcggcga
     2221 ccaccgtgac gtcgcagaag ttcgagccca cgactgatcg ggtagtaggg cgctgacctt
     2281 cgtcgagcac gcgcacgaac ggctcgcccg cgtagaactc ctggtagagc gccaccagct
     2341 ccgcagtcgt ggccggcttc gtgagcggca ccgacgcggt ggtgaagaga ccgcggttga
     2401 gcgggaccag gtgcggcgtg aacgcgacca ccaccggccg gcccgccagc agggacagct
     2461 cctgctcgat ctccggagtg tgccggtggg tggctatgcc gtaggcctgc acgttctcgt
     2521 tcgcctcggt gaagaggtac atcgcgtcgg ccttgcggcc ctgggcgccc gcgccggtca
     2581 cgccggattt gccgtcgatc acgatgccgt ccacgctccc gcgcccgctc ttgagcagcg
     2641 gcgcggtggc gaggatcgcg cccatcgggt agcagccggg ggaggccacc agcgacgcgc
     2701 ccgcgacctg cttgcggtgc agctcgggca ggccgtagac cgcctcggtc agcccgctcg
     2761 catctgcgtg gggcgccttg taccacgtcg tgtacagcga cgcgtccttc aggcggtagt
     2821 cggcggagag atcgaccgcc ttcttgccgt ggcgcctcag cgtgggcatc agccgctggg
     2881 attccatgtg cggcagcgcg aggaagacga cgtcggctag ggcggaaagg cgctccccgt
     2941 cgatctcctg gaacacgaga tcgctgagcc cgcgcaggtg cgggtagagg cgatcgaggc
     3001 gctcccccgc cagccgctcg gaggtgacgg cgctgatccg caccttcgga tggacggaca
     3061 ggaggcggag cagctccgcc cccatgtacc cgctggcccc ggtgaccgcg actttcaaca
     3121 tggcggcctc gcgggcgcct accgcttcga gtactggaac ttggagcgcg cgcccggctg
     3181 accgtacttc ttccgctcct tcatccgcgg atcgcgggtg aggaggccgg cccgcttgag
     3241 ggtctggcgc agcttgtcgt cgaactgcag gagggcccgt gcgatcccgt ggcgcaccgc
     3301 ccccgcctgg ccggtggggc cgccgccgcc cacgttcacg atggcgtcga actgcccaaa
     3361 cgtgttcgtg agctgaagcg gctgcgagat gaccatgcgc agcgtctcgc gcgggaaata
     3421 gtcctcgaag gggcggcgat tgaccagaat gcggccgctg cccgcccgca gccagacgcg
     3481 ggcgacggag gtcttgcggc gaccggttcc atagaaacga ctgacgacgg ccatggggct
     3541 cctcagcggg cgcgctcgcg catggcgagc ggctcgggct tctgggcggc gtgcgggtgg
     3601 tccgccccct tgtagacctt cagcttcttg gccatcgcgc ggcccaggcg gcccttcggg
     3661 agcatgccct gcaccgccca ctcgatcacg cgctcgggat gcgtcttgag catccgctcc
     3721 gcgctgacct cacgcagtcc cccgatgtat ccgctgtgcc agcggtagat cttgtcggtc
     3781 agcttgcgcc cggtcaggtg caccttctcg gcattgatga ccaccacgtg atcgcccacg
     3841 tcgaggtgcg gcgagaactg gggcttgtgc ttgccccgca gcaccgtcgc cacctgggtg
     3901 gcgagacggc cgagcacccg gtcctgggca tcgaccacga accacttgcg ctccacatcc
     3961 tcttccttcg gcatcagcgt cgtcatgatc tccgtccgga tagggcctct accgccacgt
     4021 cgaacacccc ggcaagcggc aacgggtaat cataaaagaa agactccggc cggtcaacta
     4081 ccctgcccgg cggcgggctc caggcccggc gggggccgcc ttcgtcttgc ccggccacgg
     4141 gcagagggcc aggatcgggc acgtcgggca ggccggcttg cgggccgtgc agacgcgccg
     4201 cccgtggagg atgagcaggt ggctggcctg cgtccagcgc ggacgcggga ggacctggca
     4261 gagctggtcg tggaccttct cggcatcgtc gccccgggcc agccccaggc gctgcgacac
     4321 ccggaacacg tgggtgtcca ccgccagcgc cgggatgccg aaggcgttgc ccagcaccac
     4381 gttggcggtc ttccggccga ccccgggcag ctccaccagc tcctcgagcg tccggggcac
     4441 taggcctccg tggcgctcca ccagcgcgcg ggccatcccg ttgagcgact tggccttggc
     4501 gcggaagaac ccggtggaat ggatctcgcg ctcgaggacc ggcagcggca cccccgccca
     4561 gtccgcggcc cggcgatagc gcgggaagag cgcggcggtg accgcgttga cgcgctcgtc
     4621 ggtgcactgc gcggacagga tcgtcgcgac cagcagctcg agcggcgtgg tgaaatcgag
     4681 cgcggtgcgc gcgtccgggt aggccttcgc cagccgggtc aggatcttgc ccgcccgcgc
     4741 cttcttgtcc gcgagactct cggccatcag cgtgactcct ttgtcgcggc ggtctcggct
     4801 ccatcctcgc cgacctcgag ccgctcccgc cggatgtgga tcggccccat cgacaccggt
     4861 atgggcagcc ggcgcaggcg cgggctcggg tcgaagtttc tcacgatcca gtccaccagg
     4921 agccgcggca gacgcacccc gccgtagcgg acgtcggcgg ctcgcagagt gaacggatcg
     4981 ggcggcgtgc cggcttccag ggccagccgc gcggatagcc caggtcccgg cagcgcccag
     5041 cgcacctgga tccatccctc gtccatggtg gcgcgcagcc cggccaggcc gcgctgctgg
     5101 gcgatgaact cggccagatc ccgctcggcc accccaagcc gctccacgtg gagggatccg
     5161 atgtcgagga gctcgacggc gccggtggcc gcgagccggc ccggattcac gaccaggccg
     5221 cggacgcgca ggcgcaccgg gccgatgcgc agcggcggct tccgccgagc gaactcgccg
     5281 accagcgccg actgcgcctc caccgtcacc ccgtccaccg atcccgccgc cagcgcctca
     5341 tctcgatagt cgagcgtcac gcggaggccc ttggcatcgc ggacgacgtc ggccaggagc
     5401 ccggccaccc cgcgccgcag ccgctccgcg agctccgccg gcgccactcc gcggaccggc
     5461 tccgggcggc gcatccgcac catgccctcg ctgccatccg gcagcggcac ccgcgcgatc
     5521 acggggtacg ccgcagccag ccatggatcc ccgcctccga gcgcggccat gatccggtcg
     5581 ggcttggcgg aggtcgcgtc ggggccctgg tcacccgtct tcacgatggc gaagtccaca
     5641 ccgagcgggg ccccctccca cgcgcggctc aggcggagcg gcaaccgatc gcgcgtcacc
     5701 tcgtagcgaa agttggagac ggagaagaaa ttgtcgttgg gcaccaccgc cacccgcgct
     5761 tcccgggggc ccgattcgcg caggacggcc tcgagaatct cccggtgatg ccagtcccgc
     5821 ccatcgggcg gcgaggagaa tgccatcgag aagacgaccg gcgccagcag cgtagggggc
     5881 ggcagcgcga aggccgcgga gcccacctgc gcgaccccca tgatcgcgca cgcccagctc
     5941 aggccccgcc ggacacgcgg tgggagcacg ctcacgccgg ccgccgccac cagcgcggcc
     6001 gcgggcagga gcggcagggt gtagcgcagg ttcttgttct ggatgaggac gaagatcagg
     6061 aatggcgcca gcgcggccca caggagcccc cgggcctgcg gccggcgcgc gagcgccacg
     6121 atgccccata gcaggagcgc cgtcgccagg agaccccact gggggatgat ccactcggga
     6181 tagaacagca gcgatgcgcc actgagcgcc ggcgcttgcc ccgcttcggc cgcctgcttg
     6241 aacgagcggt tgatgaactg ggccggcaac ccgaccaggc gcggcccgta ccatggcagc
     6301 gcgaccgccg ccgccgtccc caccgcggcg agcagatgga gcaggcgggc ccggcggtcc
     6361 ggcgcccgga gcccccgcca tgcgccccag aggagcgggc ccgagagata ggcggcgaag
     6421 ggcggcttgg tgagcatgcc caggccgagc accaagccca gcgcgagaac ccacgggcgg
     6481 accgccaggt cctcggtcct caccagcacg tacagggcca gcgccaccac ggcggccagc
     6541 ggcaggtcga gctggaagtt cagcagcatg aacacgacga agggcgcggt ggcgaagagg
     6601 aaggcggcca gcaggcccgc gtcggcgtcg aggagccgcc ggccgaggcc gaagacggcg
     6661 agcgtgccga cgacgaggaa ggcccagatc acggcctggg cggtcagggg aacgatgggc
     6721 aaggcgaagt agaggaggcc ggccgcgcac gtcacgatcg gcgggtagaa gctcgactcg
     6781 cggatgatct cctgcagcca gtcgcccccg gactccccga ggctccggtg acaggcgacg
     6841 gcgcgctcga gatggttggc atgatcccat tcgggcgggc ggcgatcgat cccgagccag
     6901 acgaccatga tcgcggccag cccgatcagc acagcagcca gcgcgagccg gtgccggccg
     6961 gtcatgaatc cgggcccatg acggtcaggc gagggcccgg cgtatcatct cgatccgctc
     7021 gcgcgccgcg gcggg
//
LOCUS       DASUFA010000284         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_168830, whole genome shotgun sequence.
ACCESSION   DASUFA010000284 DASUFA010000000
VERSION     DASUFA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168830"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2196
                     /locus_tag="VFX87_10665"
     CDS             <1..>2196
                     /locus_tag="VFX87_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX4995425.1"
                     /translation="NRLLRGAADTTFLPSPSPPPEGVRYVITLDADTHLPRGAAARLV
                     GTLAHPLNRARLDPYEHRVVEGYGILQPRIAPTLPTDREGSIYQRISSGPAGIDPYAS
                     AVSDVYQDLFGEGSYTGKGIYDIDAFERALAGRVPEGALLSHDLFEGLFARAGLVSDI
                     DLFEEFPARYEVAAARQHRWARGDWQLLPWILRGSAGEGAARGPIDTIGQWKMFDNLR
                     RTLSAPATYLTLLLGWTLSDGVALVWTKFVLLAIAIPAFIPVSTEILPQRPGISKRAY
                     LRSLAGSLMLAVAQVTLTFVLLAHQAWLMADAVGRTLTRLVFTRRRLLEWVSAAQVKS
                     SSTLDLGSVYARMSGALVLALVAGVVAMLAPGAHWILAVPLVTFWFLSPAVARWISLP
                     PRARPAEQLAPDERETLRTTARGSWRFFETFVTAADNALPPDNFQEEPRPVVARRSSP
                     TNIGLYLLSTASARDFGWIGTLDMADRLEATLATLDRLERFRGHFYNWYDTGDLRPLE
                     PRYVSTVDSGNFAGHLCVLGVAAREWTARPFVDAEAARGVVDALRLVRDAASLTPHAA
                     SDRLRAASERLIDGLAGPPGTAGAWSARLRELTADAEALTMLAQSLGGDSREAALAWV
                     KAAQTTIGGHRRDLEALAPWAEPLDALAADLPEEMLVGREAVGRLRSETPALGDLAAC
                     TEAAARELTALVAEGVPPGVDAVLDAGRVGKVIEALERSGLAAAALAHR"
BASE COUNT          263 a          835 c          770 g          328 t
ORIGIN      
        1 tcaaccggct cctgcggggc gccgccgaca ccaccttcct cccgtcgcct tcccctcccc
       61 ccgagggcgt ccgctacgtg atcacgctcg atgccgacac ccatctgcct cggggcgcgg
      121 cggcgcgcct ggtgggaacg ctcgcccacc cgctcaaccg ggcgcggctc gatccgtacg
      181 agcatcgggt ggtcgaaggc tacggaatcc ttcagccccg catcgcgccc actctgccca
      241 ccgatcgcga gggctccatc taccagcgga tctcgtcggg tccggcgggg atcgacccgt
      301 acgcctcggc ggtgtcggac gtctaccagg atctcttcgg ggagggctct tacaccggca
      361 agggcatcta cgacatcgac gccttcgagc gcgcgctcgc cggccgggtg cccgaaggcg
      421 cgctcctgag ccacgacctg ttcgaaggac tgttcgcgcg ggccgggctc gtgagcgaca
      481 tcgacctctt cgaggagttc cccgcgcggt acgaggtcgc ggcggcgcgg cagcaccgct
      541 gggctcgggg cgactggcag ctcctgccgt ggatcctgcg gggatcggcg ggcgagggag
      601 ccgcgcgcgg gcccatcgac accatcgggc agtggaagat gttcgacaac ctgcgccgga
      661 cgctctccgc gcccgccacc tacctcacct tgctcctggg ctggaccttg tccgacggcg
      721 tcgcgctggt ctggaccaag ttcgtcctgc ttgccatcgc cattccggcg ttcatcccgg
      781 tgtcgacgga gatcctgccg cagcggcccg gcatctcgaa acgcgcctac ctgcgaagcc
      841 tggccgggag tctcatgctg gcggtggccc aggtcaccct caccttcgtg ctcctcgccc
      901 accaggcctg gctgatggcc gacgcggtgg gccgcacgct gacccgcctc gtcttcacgc
      961 ggcgccggct tctcgagtgg gtctccgcgg ctcaggtcaa gtcgagctcg accctcgatc
     1021 tgggcagcgt gtacgcgcgc atgtcgggcg cgctcgtgct ggcgctcgtc gcgggggtgg
     1081 tcgcgatgct ggccccgggc gcccactgga tcctcgccgt cccgctcgtc accttctggt
     1141 tcctctcgcc cgcggtggcc cggtggatca gcctgcctcc ccgcgcgcga ccggcggagc
     1201 agctcgcccc ggacgagcgc gaaaccctgc gcaccaccgc gcggggaagc tggcgcttct
     1261 tcgagacgtt cgtgaccgcc gcggacaatg cgctgccgcc ggacaatttc caggaggagc
     1321 cccgaccggt ggtggcccgg cggagctcgc ccaccaatat cggtctctac ctgctctcca
     1381 ccgcgtccgc tcgggacttc ggctggatcg gcaccctcga catggcggac cggctcgagg
     1441 ccacgctcgc gacgctggac cggctcgagc gcttccgcgg gcacttctac aactggtacg
     1501 acaccggcga cctccggccc ctcgagcccc gctacgtctc caccgtcgac agcggcaact
     1561 tcgccggaca cctttgcgtg ctcggcgtgg cggcgcgcga gtggaccgcg cggcccttcg
     1621 tggacgccga ggcggcccga ggcgtggtgg acgcgctgcg gctcgtccgc gatgccgcgt
     1681 ccttgacgcc ccacgccgcg tccgatcgtc tgcgcgcggc gtcggagcgg ctgatcgacg
     1741 gcctcgcggg gccgcccggc accgcgggcg cctggtcggc ccgactgcgc gagctgaccg
     1801 cggatgccga ggcgctcacg atgctggcgc aatcgctcgg cggggactcc cgcgaagccg
     1861 cgctcgcgtg ggtgaaggcc gcgcaaacga cgatcggggg ccaccgtcgc gacctcgagg
     1921 cgctggcgcc ctgggccgag ccgctcgacg cgctcgccgc cgacctgccg gaagagatgc
     1981 tggtcggccg ggaggcggtg gggcgcttgc ggagcgagac ccccgcgctc ggcgacctgg
     2041 cggcctgcac cgaagcggcc gcccgcgagc tgaccgcgct cgtcgccgag ggcgtgccgc
     2101 ccggcgtcga tgcggtgctg gacgccggcc gcgtgggcaa ggtgatcgag gccctcgagc
     2161 ggtcggggct ggccgcggct gcgctggcgc accgcc
//
LOCUS       DASUFA010000285         9015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_873524, whole genome shotgun sequence.
ACCESSION   DASUFA010000285 DASUFA010000000
VERSION     DASUFA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873524"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..515
                     /locus_tag="VFX87_10670"
     CDS             93..515
                     /locus_tag="VFX87_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995426.1"
                     /translation="MKTIKALVLATATFGALGQALGLALGPIALPTSAAAVSAPSGID
                     PRIRLDWEAGTRRGGKPVIQGYVYNDYGRPATDVHLLVETLDASGAVIAKTIGFVQGV
                     VQFNDRVYFEVPIRVTGASYRVTVTSFDWKGGGGGGGM"
     gene            complement(527..2119)
                     /locus_tag="VFX87_10675"
     CDS             complement(527..2119)
                     /locus_tag="VFX87_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4995427.1"
                     /translation="MRFRLTAKITVLIILVLIIGFGVATVLTIRRESELLVEQNKMAA
                     RQLIGTLVSSIETAMLSERPDITRGLIDDLRSSKLVQGLVVYRRNGVEAFTDLATLRE
                     VSKEAELSKDVMASIEKMRREPGRTMAGPLFTRALETLQTQEALESREGASVFTVYQP
                     IPNQERCQGCHGSDHKVRAVVQAATSMEPVMAEVRKQRNRQIMIGLMTIVAAAAVLAV
                     AMRYVVVRPIHDLAAVARRIGEGDFAVRARAGSGDEVAELGGALNEMTGHLELAHQNL
                     AARNTELATALENLQASRQRLEVLEQLKGELSKFVPDAVKELLERDPSATSLEKRNEE
                     VSVLFLDIAGYTRLSEQLEAKRLNQLVQTYFSSFLEIIRRHHGDVNETAGDGLMVIFQ
                     KQPRGPGGGDEDHALNATRAAFAIRQKTTALNEEYGGVFPAIALHMGINTGQALVGAT
                     KLGGAASQRWTFTATGPTTNIAARFAGAAEGGDIVVGQVTADRIAGLFVLESLGERSF
                     KNVSQPLPIYRVIPPGVYSKVT"
     gene            complement(2130..3659)
                     /locus_tag="VFX87_10680"
     CDS             complement(2130..3659)
                     /locus_tag="VFX87_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4995428.1"
                     /translation="MDVLAAHAARHPGKAALLEGARAWTWAEFVARRNRLAHGLVGLD
                     LPPGGHVIVYAENSLEHYLAGAAARVAGLIPAPMNHRLVAEEVLYILDHSDAVAVLVS
                     DRFLPLVETIRVPAGKVRRWILLGAERRPWAVHMDDLLAVGRPEPVEPRAGEGFGASI
                     IYTGGTTGKPKGALRRGINPQDLMDTLRAMDLLDPAHVHLVAGPMYHSAPGGLALYTH
                     LVGGTVVIMPKFDPEQALAEIERHRCTSTFMAPTLLKRIIDLPAAVRARYDVSSMRAI
                     IMAAAPCPMSVKEGVMAHFGPALYEFYGSSELGVNTVLRPEDVLRKPGSCGRAAPGKE
                     IALLDDDGRPVPTGEPGELYVRQFPGILDEYYRDPEATLKMRRGDWYTVGDVAYVDAD
                     GFYYICDRKRDMIISAGVNIYPAEIEDVLHRHPRVLDAAVFGVPDEEWGERVHAAVHV
                     RPGESLTAEEITAFCREHMAGYKVPREVSFHDVFPRDAAGKLLKRQLREPYWAGRASR
                     V"
     gene            3792..4247
                     /locus_tag="VFX87_10685"
     CDS             3792..4247
                     /locus_tag="VFX87_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4995429.1"
                     /translation="MAADRSYVAENRAQLERLRALTDRLSDQQLAAPMEAGWTVAGVL
                     AHLAFWDQRIVTLLDRWAGGRGVPPPAYDEAAVDWINDAGKPLCLALPPRVAARMAVE
                     AAIAADERVAGLSEAQLAENAAAGSPISVLRAIHRREHLNEIEQILARR"
     gene            complement(4254..5156)
                     /locus_tag="VFX87_10690"
     CDS             complement(4254..5156)
                     /locus_tag="VFX87_10690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX4995430.1"
                     /translation="MTQLRSKEHDARAIAQHYDVSNDFYALFLDPLMVYTCAYYRDAD
                     GKLEQAQEDKLDLVCRKLRLQPGERVLDIGCGWGGFSIWAAQHYGVRAHGVTLSKAQA
                     EWAAARIKREGLEDRCLVEYRDVRDLPADARYDKIAAIGVIEHVGIPNYPSFFGRVKE
                     MLVDGGLYLNHGIHHEFHWKRTSQTDFLYRYVFPNGDLSGVTETLTEMERAGWELLDL
                     ENLRPHYARTCRQWAERLKARADEARAIVGEQVYRTWLLYLTCSSVAFATGSIGLYQV
                     LMQKQWDRTRGTAPTTREAIYTGW"
     gene            complement(5153..6277)
                     /locus_tag="VFX87_10695"
     CDS             complement(5153..6277)
                     /locus_tag="VFX87_10695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4995431.1"
                     /translation="MDHDAIVVGGGPGGSTAAWRLALAGLRPLVLDAAVFPRVKICAG
                     WVTPEALADLEVDPDKYPLTIQPFDQCVLEFAGARHETGWRRPTSYGIIRREFDHYLL
                     ERAAAAGADVRWGIRVTGVAEQADRVRVETDRGIFEAPVVIGAGGHRCPVARVLGHVS
                     GREEVVVAQESETRLPPEWVARLEPFLHAPELYVEPDLRGYGWYFPKRDFINIGVGCT
                     QGGDGSLPRRRDALVAALRASGRLPDGLPIEPFKGHAYVVRRQAPRRLSGARFCLIGD
                     AAGLARDVSGEGIGPAIRSGRLAAEAVTALIRHGTPLDAYARQIVARYGPGERGWIRQ
                     KLSNLPDALARLGVRVVLGSAAARRRIVFDSIFGMREVAP"
     gene            complement(6277..6624)
                     /locus_tag="VFX87_10700"
     CDS             complement(6277..6624)
                     /locus_tag="VFX87_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995432.1"
                     /translation="MLAFLTPPPTPARAAAILRKVFGSLPASFAFRLWDGQEVRFGSD
                     RPECTAVIKSPETFLSLIRDPSPYNFAEAYVQSRIDLEGDLFGAMDVANVVENLKVTP
                     LQKLSILLSLWRR"
     gene            complement(6624..7343)
                     /locus_tag="VFX87_10705"
     CDS             complement(6624..7343)
                     /locus_tag="VFX87_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995433.1"
                     /translation="MLELEGLNVFRGPAQVLRNLSLAVADRESVCLVGRNGAGKTTTI
                     DGIMGLLPVRSGRVALGGRDITKLPTHQRALAGIGYSPEDAGIFPDLTVAENFRISQS
                     LARAAGKGGAVLAGNGIDERVLRLFPEIGDFTGRRGLFLSGGQKKMVAIGRAMTLSPS
                     ILLLDEPFEGLAPVVVSRFIDAVKQIKAMGISLLIAESNLMTASRVADRLYAIDRGEI
                     IFEGEPRRAFENPDVMKTIRG"
     gene            complement(7347..8099)
                     /locus_tag="VFX87_10710"
     CDS             complement(7347..8099)
                     /locus_tag="VFX87_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995434.1"
                     /translation="MDILRTTQLCKSFGETHAVDHVDFTVTAGEVLALIGSNGAGKTT
                     LINLISGLIPPDSGRIEFQGADITRASVHHKIELGIARSFQLVNLFDQLSVHDNVTLA
                     IFSREGRTRRLFSLAAADTAVRDEAVTVLKQFGLDARTAAPAGGLSQGERKLLDVAVA
                     YALRPKLLFLDEPTSGVSTREKAPIMDIITAVVRSGGITAVIVEHDMDVVFTYCPRIV
                     AMHQGAILADGTPDQIRNHPQVTANLLGAQTA"
     gene            complement(8102..>9015)
                     /locus_tag="VFX87_10715"
     CDS             complement(8102..>9015)
                     /locus_tag="VFX87_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4995435.1"
                     /translation="LPYVVPPYQTVLLTYGLVFAIAALGFNLLLGYTGLLSFGHSAYF
                     GVGAYAVAFIVKYLKLESMEAFLLGGILASGAVAALFGLLCVRYTKIFFGILTMALSQ
                     VLWSLAFKFFWVTGGSDGLRVPTPTLLGGYVRAGGDKFEFLSHRYYFYVLAVFAVCVA
                     VMWVIVHSPFGKALQAIRDNETRAEFVGVQVWHYRWVAFLISGMFTGLAGALWVPLNG
                     LTTPDILHWTFSGKMVFFTVLGGFQTFFGPIIGAVIFNYLETYAVGHTVYWQMVLGIV
                     LVVLVLALPAGIVGTLRHLGLRPVKMS"
BASE COUNT         1377 a         3129 c         3212 g         1297 t
ORIGIN      
        1 ccgaggccgg gctcatggcg ggcggccggg gctgaatcgt ccgcatcccg gggtcacccc
       61 cgcgcatcat agggggcagg aggcggtcga ccatgaagac gatcaaggcg ctggtgctcg
      121 cgacggcgac attcggggcc ctgggccaag ccctgggcct ggccctcggc ccgatcgcgc
      181 tgccgacgtc cgcggcggcg gtgagcgcgc cgtcgggcat cgatccgcgg atccggctgg
      241 actgggaggc cggaacgagg cgcgggggca agccggtgat ccagggctat gtctacaacg
      301 actacggccg gccggccacc gacgtccacc tgctggtcga gacgctcgac gcctccggcg
      361 cggtgatcgc gaagacgatc ggcttcgtgc agggggtcgt ccagttcaac gatcgcgtct
      421 acttcgaggt gccgatcagg gtgaccggcg cctcctaccg cgtcaccgtg acctcgttcg
      481 actggaaagg cggagggggc ggcggcggga tgtagccgcg accacgctac gtcactttgc
      541 tgtagacccc gggtgggatc acccggtaga tcgggagcgg ctgcgagacg ttcttgaagc
      601 tgcgctcgcc gagcgattcc agcacgaaga gcccggcgat acggtcggcg gtcacctggc
      661 ccaccacgat gtccccgcct tccgcggcgc ccgcgaaccg cgccgcgatg ttggtggtgg
      721 gcccggtggc ggtgaaggtc cagcgctggg aggcggcccc gcccagcttg gtggcgccca
      781 ccagcgcctg gcccgtattg atccccatgt gcagcgcgat ggccgggaac acgcccccgt
      841 actcctcgtt cagcgcggtc gtcttctggc ggatcgcgaa ggcggcgcgg gtcgcgttca
      901 gggcgtgatc ctcgtcgccc ccgccgggcc cgcgcggctg cttctggaag atcaccatga
      961 gcccgtcgcc cgccgtctcg ttcacgtccc cgtggtgccg gcggatgatc tcgagaaagc
     1021 tcgagaagta ggtctggacg agctggttga gccgcttggc ctcgagctgc tcggagagcc
     1081 gcgtgtagcc cgcgatgtcg aggaagagca ccgagacctc ctcgttgcgc ttctccaggc
     1141 tggtcgcgct gggatcccgc tcgagcagct ccttgaccgc gtcgggcacg aacttggaga
     1201 gctcgccctt gagctgctcg agcacctcga ggcgctgccg cgaggcctgc aggttctcga
     1261 gcgcggtggc cagctcggta ttgcgggccg ccaagttctg atgggctagt tcaagatggc
     1321 cggtcatctc gttgagcgcg ccgcccagct cggccacctc gtcgccggag ccggcgcgcg
     1381 cccggaccgc gaagtcgccc tcgccgatgc gacgggccac cgcggccaga tcgtggatcg
     1441 gccgcaccac cacgtagcgc atggccaccg ccagcaccgc ggccgcggcc acgatggtca
     1501 tgaggccgat catgatctgg cggttccgct gcttgcgcac ctccgccatc accggctcca
     1561 tggaggtggc cgcctggacg accgcgcgca ccttgtggtc cgagccgtgg cagccctggc
     1621 agcgctcctg gttggggatc ggctggtaga ccgtgaacac ggaagcgccc tcgcgcgact
     1681 cgagcgcctc ctgggtctgg agcgtctcga gcgcccgcgt gaagagcggc cccgccatcg
     1741 tgcgccccgg ctcgcggcgc atcttctcga tcgaggccat cacgtccttg gagagctcgg
     1801 cttccttcga cacctcccgg agcgtggcca ggtccgtgaa ggcctccacc ccattcctcc
     1861 ggtagacgac gaggccctgg acgagctttg agctgcgtaa atcgtcgatg aggccgcggg
     1921 tgatgtcggg ccgctcggag agcatggcgg tctcgatgct cgagaccagg gtgccgatga
     1981 gctggcgcgc cgccatcttg ttctgctcga cgagcagctc gctctcgcgc ctgatggtga
     2041 ggacggtcgc gactccgaac ccgatgatca gcaccaggat gatcaggacg gtgatcttgg
     2101 cggtgaggcg gaagcgcatg cgcggagact cagacgcgcg aggcgcgccc ggcccagtag
     2161 ggctcgcgga gctggcgctt gagcagcttg cccgccgcgt cgcgcgggaa gacgtcgtgg
     2221 aaggagacct cgcggggcac cttgtagccg gccatgtgct cgcggcagaa cgcggtgatc
     2281 tcctccgcgg tgagactctc cccggggcgc acgtggaccg cggcgtgcac gcgctcgccc
     2341 cactcctcgt ccggtacccc gaagacggcc gcgtcgagca ccctggggtg ccggtgcagc
     2401 acgtcctcga tctcggcggg gtagatgttg acgcccgccg agatgatcat gtcgcgcttg
     2461 cggtcgcaga tgtagtagaa gccgtccgcg tccacgtagg ccacgtcgcc caccgtgtac
     2521 cagtcgccgc ggcgcatctt gagcgtcgcc tcgggatccc ggtagtactc gtcgaggatg
     2581 ccggggaact ggcgcacgta gagctcgccc ggctccccgg tcggcaccgg ccggccgtcg
     2641 tcgtcgagca gcgcgatctc cttgcccggc gccgcgcgcc cgcaggagcc cggcttgcgc
     2701 agcacgtcct ccggccgcag caccgtgttc accccgagct cgctcgagcc gtagaactcg
     2761 tagagcgcgg ggccgaagtg ggccatcacg ccctccttca cgctcatcgg gcacggggcc
     2821 gccgccatga tgatggcgcg catcgaggac acgtcgtagc gcgcccggac cgccgcgggc
     2881 aggtcgatga ttcgcttgag gagtgttggg gccatgaagg tgctggtgca gcgatgccgc
     2941 tcgatctcgg ccagcgcctg ctccgggtcg aacttcggca tgatgaccac ggtgccgccg
     3001 accaggtggg tgtagagcgc gagcccgccc ggcgccgagt ggtacatggg cccggccacc
     3061 aggtgcacat gggccggatc gagcaggtcc atggcccgca gcgtgtccat caggtcctgc
     3121 ggattgattc cccgacgcag ggcccccttg ggtttcccgg tggtcccacc ggtgtagatg
     3181 atggacgcgc cgaagccctc gccggcccgc ggctccaccg gctcgggccg gcccacggca
     3241 aggaggtcgt ccatgtgcac ggcccagggg cgccgctcgg cgccgagcag gatccaccgc
     3301 cgcaccttcc cggccgggac ccggatcgtc tcgaccagcg gcaggaagcg gtcgctcacc
     3361 agcaccgcga ccgcgtcgga gtggtcgagg atgtagagaa cctcctccgc gacgagccgg
     3421 tggttcatcg gcgcggggat gagtccggcc acgcgcgcgg ccgcgccggc caggtagtgc
     3481 tccagggagt tctccgcgta gacgatgacg tggccgccgg gcggcaggtc caggccgacg
     3541 aggccgtggg ccagccggtt gcgccgggcc acgaactcgg cccaggtcca ggcgcgcgcg
     3601 ccttcgagga gagcggcctt gccgggatgc cgggcggcgt gcgcagcgag aacgtccatc
     3661 gctcccgcgt gtgtagcacg aatcgggcgg aggcggcaac cgatggccac ttcacacgcg
     3721 ccgtcgatcg gtgtcagaat gatgcgggct ccatcacggc gggcccaggc ccatcacgag
     3781 ggaggttgtc catggcggcg gaccgatcat acgtggccga gaaccgggcg cagctcgagc
     3841 gactccgcgc cctcaccgac cggctcagcg atcagcagct ggccgcaccg atggaggcag
     3901 gctggacggt ggccggggtg ctggcgcacc tggccttctg ggaccagcgg atcgtgacgc
     3961 tgctcgaccg ctgggccggg ggacgcggcg ttccgccgcc ggcctacgac gaggcggcgg
     4021 tggactggat caacgatgcg ggcaagcccc tctgcctggc cctgcccccg cgggtcgccg
     4081 cgcggatggc ggtggaggcg gcgatcgccg cggatgagcg cgtggcgggc ctgtcggagg
     4141 cgcagctcgc ggagaacgcg gccgcgggca gcccgatcag cgtgctgcgc gccatccacc
     4201 ggcgcgagca cctgaacgag atcgagcaga tcctggcgcg ccgctagcgg aacctaccag
     4261 ccggtgtaga tcgcctcgcg ggtggtcggc gcggtcccgc gcgtccggtc ccactgcttc
     4321 tgcatcagga cctggtagag gccgatcgaa ccggtcgcga aggccaccga cgagcacgtg
     4381 aggtagagga gccaggtccg atagacctgc tcgcccacga tcgcccgtgc ctcgtccgca
     4441 cgggccttga gacgctccgc ccactgccgg cacgtgcgcg cgtagtgcgg ccgcaggttt
     4501 tccaggtcca gcagctccca gcccgcccgc tccatctcgg tgagggtctc ggtgacgccg
     4561 gagagatcgc cgttggggaa cacgtagcgg tagaggaagt cggtctggct ggtgcgcttc
     4621 cagtggaact cgtggtggat gccgtggttg aggtagaggc ccccgtccac cagcatctcc
     4681 ttcacccggc cgaagaagga cggatagttc gggatgccca cgtgctcgat gaccccgatc
     4741 gccgcgatct tgtcgtagcg cgcgtccgcg ggcaggtccc ggacgtcgcg gtactcgacc
     4801 aggcaccggt cctcgagccc ctcccgcttg atccgggcgg ccgcccactc ggcctgcgcc
     4861 ttcgagagcg tgaccccgtg ggcgcggacc ccgtagtgct gggcggccca gatgctgaag
     4921 cccccccagc cgcagccgat gtcgagcacc cgctcgcccg gctgcagccg gagcttgcgg
     4981 cagacgagat cgagcttgtc ctcctgcgcc tgctcgagct tgccgtccgc atcgcggtag
     5041 tacgcgcagg tgtagaccat cagcgggtcc aggaacagcg cgtagaagtc gttcgagacg
     5101 tcgtagtgct gcgcgatcgc tcgggcgtcg tgctccttgg accgaagctg tgtcatggcg
     5161 ccacctctcg catgccgaag atggaatcga acacgatccg tcgccgggcc gccgccgagc
     5221 ccaggaccac ccgcaccccg agccgggcca gcgcgtctgg aagattcgag agcttctgcc
     5281 ggatccagcc gcgctcgccg gggccgtagc gcgccacgat ctgccgcgcg taggcgtcga
     5341 gcggtgtgcc atggcggatc agggccgtca ccgcctcggc ggccagccgg ccgctccgga
     5401 tggcggggcc gatcccctcc cccgacacgt cgcgggcgag cccggccgca tcgccgatga
     5461 ggcagaagcg cgcgccggaa agccgccggg gggcctgccg ccgcacgacg tacgcgtgcc
     5521 ccttgaacgg ctcgatcggc agcccgtccg gcagccggcc cgaggcgcgc agggcggcca
     5581 ccagcgcatc gcggcgacgg ggcaggctcc cgtcgccgcc ctgggtgcac cccacgccga
     5641 tattgatgaa gtcgcgcttg gggaagtacc agccgtagcc gcgcaggtcg ggctcgacgt
     5701 agagctcggg cgcgtgcagg aacggctcga ggcgcgcgac ccactcgggc ggcagccgcg
     5761 tctcgctctc ctgcgccacc acgacctcct cgcgcccgga gacgtgcccg agcacccgcg
     5821 ccaccgggca gcgatggccg cccgcgccga tcacgacggg cgcctcgaag atcccgcggt
     5881 cggtctcgac gcgcacccgg tcggcctgct cggccacgcc ggtgacccgg atgccccagc
     5941 gcacatccgc gcccgcggca gccgcgcgct cgagcaggta gtgatcgaac tcgcggcgga
     6001 tgatgccgta gctggtgggc cgccgccagc ccgtctcgtg gcgggcgccc gcgaactcca
     6061 gcacgcactg gtcgaacggc tggatggtca gggggtactt gtcgggatcc acctcgaggt
     6121 cggcgagcgc ttccggggtc acccagcccg cgcagatctt cacccgcggg aagaccgcgg
     6181 cgtcgagcac gagggggcgc aggcccgcca gggccagccg ccaggccgcg gtgctcccgc
     6241 ccgggccgcc tccgaccacg atcgcatcgt gatccatcac cgcctccaca gcgacaggag
     6301 gatggacagc ttctggagcg gcgtcacctt caggttctcg accacgttgg ccacgtccat
     6361 cgccccgaac agatcgcctt ccaggtcgat ccggctctgc acgtaggcct cggcgaaatt
     6421 gtacggtgaa gggtcgcgga tcagcgacag gaacgtctcc gggctcttga tgaccgcggt
     6481 gcactcgggc cggtccgagc cgaagcgcac ctcctggccg tcccagagcc ggaaggcgaa
     6541 cgacgcgggc aggctgccga agaccttccg aagaatggcg gcggcccgcg cgggggtcgg
     6601 cgggggggtc aggaaggcca gcatcacccg cggatcgtct tcatgacgtc ggggttctcg
     6661 aatgcacgcc tgggctctcc ttcgaagatg atctccccgc ggtcgatagc atacaaccga
     6721 tcggccaccc gggaggcggt catcaggttg gactcggcga tgaggagcga gatccccatg
     6781 gccttgatct gcttcaccgc gtcgatgaac cggctcacca cgacgggggc cagcccctcg
     6841 aagggctcgt ccagcagcag gatggagggc gacagcgtca tggcccggcc gatcgccacc
     6901 atcttcttct ggccgccgga gaggaagagc ccgcgccggc cggtgaagtc gccgatctcg
     6961 gggaagaggc gcagcacgcg ctcgtcgatg ccgttgcccg cgagcacggc gccccccttc
     7021 ccggccgccc gcgcgagcga ctggctgatg cggaagttct cggccaccgt gaggtcgggg
     7081 aagatcccgg cgtcctccgg cgagtagccg atccccgcga gcgcgcgctg atgcgtgggc
     7141 agcttcgtga tgtcgcgccc tccgagggcc acccggccgc tgcgcacggg cagcaggccc
     7201 atgatgccgt cgatggtggt cgtcttgccc gcgccgttgc gcccgaccag gcacaccgac
     7261 tcgcggtccg cgacggccag cgagaggttg cgcagcacct gcgcgggacc gcggaagacg
     7321 ttcagcccct cgagctcgag catgggtcag gcggtctggg cgccgagcag gttcgcggtg
     7381 acctgcgggt ggttccggat ctggtccggg gtgccgtcgg ccaggatcgc gccctggtgc
     7441 atggcgacga tccggggaca gtacgtgaag accacgtcca tgtcgtgctc gacgatgacc
     7501 gcggtgatgc cgcccgagcg gaccaccgcg gtgatgatgt ccatgatcgg cgccttctcg
     7561 cgcgtgctga ccccgctggt cggctcgtcc aggaagagca gcttgggccg gagcgcgtag
     7621 gccaccgcca cgtcgagcag cttccgctcc ccctgggaca ggccgcccgc gggggccgcg
     7681 gtgcgggcgt cgagcccgaa ctgcttgagc accgtcaccg cctcgtcccg caccgcggtg
     7741 tcggcggcgg ccagcgagaa cagccgccgc gtgcggcctt cacgggagaa gatggcgagg
     7801 gtcacgttgt cgtgcacgct gagctggtcg aacagattca cgagctggaa gctgcgcgcg
     7861 atcccgagct cgatcttgtg gtggacggag gcccgcgtga tgtcggcccc ctggaactcg
     7921 atgcggccgg agtcgggcgg gatgaggccg ctgatcaggt tgatgagggt cgtcttgccc
     7981 gcgccgttgg agccgatgag ggccagcacc tcgcccgcgg tcaccgtgaa gtccacgtgg
     8041 tccaccgcgt gggtctcccc gaaggacttg cagagctggg tggtcctgag gatgtccacg
     8101 ctcagctcat cttcaccggg cgcagaccca gatgcctcag cgtccccacg atgcccgcgg
     8161 gcagggccag caccagcacc accagcacga tgcccaggac catctgccag tacacggtgt
     8221 gcccgaccgc gtaggtctcg aggtagttga agatgacggc gccgatgatc ggaccgaaga
     8281 aggtctggaa tccccccagc accgtgaaga agaccatctt gcccgagaag gtccagtgca
     8341 ggatgtcggg ggtggtgagc ccgttgagcg gcacccacag cgcgccggcc agcccggtga
     8401 acatcccgga gatcaggaag gccacccagc ggtagtgcca gacctgcacg cccacgaact
     8461 cggcgcgcgt ctcgttgtcc cggatcgcct ggagcgcctt gccgaagggc gagtgcacga
     8521 tgacccacat caccgccacg cagaccgcga agaccgcgag gacgtagaag tagtaccggt
     8581 gcgacaggaa ctcgaacttg tcgccgcccg cccggacata cccgccgagc agcgtgggcg
     8641 tgggcacgcg gaggccgtcg ctgccgccgg tcacccagaa gaacttgaac gcgaggctcc
     8701 acagcacctg cgacagcgcc atcgtgagga tgccgaagaa gatcttggtg tagcgcacgc
     8761 agagcagccc gaagagcgcg gccaccgccc ccgaggccag gatgccgccg aggaggaagg
     8821 cctccatgga ctcgagcttg aggtacttga cgatgaaggc caccgcgtag gcgcccacgc
     8881 cgaagtaggc ggagtggccg aaggacagca ggccggtgta gcccagcagc aggttgaagc
     8941 cgagcgcggc gatcgcgaag accaggccgt aggtgagcag cacggtctga tagggcggca
     9001 ccacgtaggg cagga
//
LOCUS       DASUFA010000286        11412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_638126, whole genome shotgun sequence.
ACCESSION   DASUFA010000286 DASUFA010000000
VERSION     DASUFA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638126"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFX87_10720"
     CDS             complement(<1..812)
                     /locus_tag="VFX87_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4995436.1"
                     /translation="MARGVRPVDRSSVALTVALLALAVLWLSPIAWVVVTSLKPAPDI
                     IRLPPEWIPWPPTGAHYHEVLLGSSRTARIGRAFVNSLVVATGSVVLVLATSALAAYP
                     LARMRFPGRDLVFVVLVGSLMIPNAVLLVPQYVLTQRLGWLSTYQGLIVPEAAMLFAF
                     GVFLLRQFFLTMPRELEDAALIDGAGPWQVFSRIILPLSQPVLAALAIFAFRSAWNDF
                     LWPLIAVNKPEMFPLPVALALLRSAYSSESYGPIMAGAALSALPLLVVFIVA"
     gene            complement(816..1169)
                     /locus_tag="VFX87_10725"
     CDS             complement(816..1169)
                     /locus_tag="VFX87_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX4995437.1"
                     /translation="MAGLQDIPRDLYEAAALDGASGWRTFWAVTLPLLRPVLLFVVVT
                     HVIGSFQLFGQVFVLTGGGPGDATRTVVQHLYEAAFQNFFQFGAASAMAWVLFAVILA
                     FSVLQFRLLRGHTEY"
     gene            complement(1176..2402)
                     /locus_tag="VFX87_10730"
     CDS             complement(1176..2402)
                     /locus_tag="VFX87_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4995438.1"
                     /translation="METPPPRLAASHALWERARRVMPGGVYGHQNGAALGEGHPRFLA
                     RAERSRVWDVDGNEYVDWMCAYGPMLLGYGHPAVEAAVAEQLAGGDCLPLPGPRMVEL
                     AERLVALTPRADFAVFGKNGADATSWAVDVARAHTGRPRIARVRGAYHAARPWALPHL
                     PGVPAHYRDDLLEFAWNDLDELGALFAAHPGEIAVVMTAPFDHLTGTAPAPGFFAGLR
                     ALCDAHGALFAMDDVRAGFRFNLGGSCEHFGAAPDLICYSKAIANGHALSAGLAREGL
                     RGAAERIYFTGSFFFASGAFAAALATLDVLARTDAIARIQRIGTLLCDGLTEQARRHR
                     IPLMPVGQPAMPVIRLADDPERRRTRRWCSLVTEGGAYAHPTHNWFVSAAHTTADVTR
                     TLEATERAFRTLAAEG"
     gene            complement(2474..3361)
                     /locus_tag="VFX87_10735"
     CDS             complement(2474..3361)
                     /locus_tag="VFX87_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995439.1"
                     /translation="MAVPVGLNIWSRLVEDTFPYFDRTVAPFDILWMPDHVQYGANKV
                     AEGWTLLTWALARYPDKRCGHEVLCNSFRNPAHLAKMAATAQALSGGRVILGIGAGWN
                     EEEYRTYGWPYPPARVRIAQLAEAIELIRRMWAGAPAEYHGEHYQISGAYCEPRPNPV
                     PPVMVGGSGEKYLLRVVARHADWWNYVWKGPEAYAHKQEVLKAHCRDVGRDYDAIEQV
                     VRVGILIAESEREVERLKQAPWIRSLNDIQIVGTPAQVTETVLGIVEQGARRLTVNFA
                     DVPRPEGTWLFASTVLPHL"
     gene            complement(3375..4355)
                     /locus_tag="VFX87_10740"
     CDS             complement(3375..4355)
                     /locus_tag="VFX87_10740"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4995440.1"
                     /translation="MTTTEAPAMLSAIIPEPAAWHGPTLPADRYLIPIPAAVLSELEG
                     ALAELRRAPVPTLLLLPEHFPLAATARWMREVRRQLDEGPAFVILDRLPVDAMSRDEA
                     VALYWLLMSFLEPPVAQEWKGTVIYDVRHDGQEYTQDTRAALTPEGLEMHTDSSMGEA
                     PPNYVSLFCLRPARAGGMSIVSSAAAAHNYFLAEHPALLRRLYEVFYRDHQEYQAADA
                     AATNFRPVFAWDGRLRTRFNARHIVRGYEKTGRALDADGAEAVRLMDAFLSDPGHRLD
                     LWVERGQIQVLNNCVIVHGRTPYQDAERPEERRHLVRLWLRAGDRRHFRG"
     gene            4521..5765
                     /locus_tag="VFX87_10745"
     CDS             4521..5765
                     /locus_tag="VFX87_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX4995441.1"
                     /translation="MTRRFVWLIVAVTGLALVAWPAIAQQSATPPAEPPKQEEVPFWA
                     IGRPKAGPGAQMAPVPAFPIPTPADKLPIGKMKVPPGFKVELFAADVFDARGLRQGDK
                     GTVFVSSLFGAGKIYALVDKGGRREVKVIAEKLMLPNGIEFHKGSLYVATPKDITRYD
                     DIEAKLDNPPAPVKVYDQLPGDVPHGWKFIKIGPDDKLYVPVGANCNICEPDPEKYAQ
                     IRRINLDGSGMEIVARGVRNTVGFDFHPKTGELWFTDNQRDWLSEDLPNDELNRVTKP
                     GQQHFGFPYCHQGDFADPQLGWGKACDDFVKPVLLLGPHAAPLGMRFYTGRMFPPKYH
                     NAIFIARHGPWNRTKKYAADVVAVFLNPNGTVKSMEPFLTGLVENNAYLGRPVDVLVL
                     KDGSLLVSDDHAGAVYRISYKK"
     gene            5762..6394
                     /locus_tag="VFX87_10750"
     CDS             5762..6394
                     /locus_tag="VFX87_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4995442.1"
                     /translation="MTASGGPGAVIPPPPARVVFVAALVLMAPLLPAARATAQSVDER
                     FTVCLACHGADGRSRIPETPSLGGQPAFFVIAQLFLFREGRRDNAPMVAAAKGLTNSD
                     LTAFAERVTRLPPPPPPEDAPDPARFARGRSLTLRHPCGVCHNPDFSGREQMPRLANQ
                     REDYLLKSMREYKSGARLGYGGAMAQELAGLTDRDLIDLAHFLSHQSVTR"
     gene            complement(6450..8804)
                     /locus_tag="VFX87_10755"
     CDS             complement(6450..8804)
                     /locus_tag="VFX87_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4995443.1"
                     /translation="MTAPESSLRFGSAAGGRRSEDGPLLTGRGRFTDDVDLPGQVHAA
                     FARSTAAHGIIRAVDTRRALALPGVLAVLTGRDAAAAGLGAIPPAAAFPGRGGKPLAV
                     APIPPLAVDRVRYVGEAIAIVVAETPAQAADAAALVGLDLDVLPAAADVDRALAASAH
                     PIWPGAARNVAFDWTDGDAAAVDAAIREAAHVARVRLHDTRLAPSALEPRAAIGQWDE
                     ASQRYTLTASTQGVAVVRRLLAEGVFKIPPQQLRVVTPDVGGGFGMKVQAYPEYAALL
                     FAARRVGRPVKWRATRVESFLADTHGRDGMLEGELALDATGRFLALRVRTRVGIGAYV
                     TAFAAVFGTNNTKNCLSSVYRIPAIAIDVTLGFTNAAPLGPYRGAGRPEALYLIERLI
                     DQAAADTGMDRVALRRRNLIPAAAMPYRTPNGPIYDSGEFETIMDRALALADWQGFPQ
                     RRATSERAGRLRGIGLACFLEVAGGILDEVVELRFADDGTVALHTGAQPMGQGHLSTF
                     PFLIAARLGIDPGAVRLVQGDSDLVPAGTPSVASRSMMMAGSAAWMACDTSIEKGRRL
                     AAHVFEAAVPDVEFANGRFRVKGTDRAIGLLDLAVRARALPDLAPDLQGGLDSTAKFV
                     SPQMSFPNGCHVTEVEIDPETGAVAVVGYAAVDDVGRIVHETIVEGQIHGGIAQGLGQ
                     VLGEHVIYDEHGQLLTASFMDYMLPRADDLPALRVGHHGVPCATNPLGVKGAGESGVA
                     GALPSAVNAVLDALAGRGVRELDMPMSPARVWAALRAAVSRPGH"
     gene            complement(8815..9594)
                     /locus_tag="VFX87_10760"
     CDS             complement(8815..9594)
                     /locus_tag="VFX87_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX4995444.1"
                     /translation="MTATALEIPSLKGKVSDEEWAVRVDLAAAYRLVSRYGWEDLVFT
                     HISARVPGTEDHFLINPYGLFFDEITASSLVKIDQEGRKLENSPFNVNAAGFIIHSAI
                     HGARHDAKCVLHTHTANGVAVSTQRAGLLPISQHSLFVLNSVGYHDFEGPALDADEKP
                     RLVADLGDNRCLILRNHGLLTVGETVADAFVNMYYLEASCQIQVRAQSGGGELISVPK
                     EVMDRGYANAVANQRRNGGQGRLVWPGLLRRLDRTDSSFRD"
     gene            complement(9608..10756)
                     /locus_tag="VFX87_10765"
     CDS             complement(9608..10756)
                     /locus_tag="VFX87_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995445.1"
                     /translation="MLERLISGDNHIDLTYCPPDLWSSQAPSKWKLLVPRVEELEDGC
                     HWFVEAQDKGMWNGVGPGFLKRTPGSFARIDEMASCGFEWDHRAGAKPRPTTPELRLA
                     DLDRDGLDAEVVYGCLMINDMIASGDRRAWANAIYNDWAADFAKKSDPNRIYPLAIVP
                     NNDPQVAAAEVRRCAKMGLKGGDLGFKSMSIPLYQHEWYPLWEAAAECHFPIAFHSTG
                     FKAVRAPDSPAMEQQYMLQWRLVRSSLFQLDTMEVLVSLITSGACEKYPDFKFVLGES
                     GVTWLPYVFDRFDTEYEDRARGLGFSMKPSDYFKRQGYVTYQQDMFLEPIIPLVGEDN
                     IIWGADYPHPDCVWPDSRRHLEKNLGNLPERVQRKITYDNVVKLYNLR"
     gene            complement(10912..11064)
                     /locus_tag="VFX87_10770"
     CDS             complement(10912..11064)
                     /locus_tag="VFX87_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995446.1"
                     /translation="MIRKLKSGQYRLYSRKKDPRTGRRRNLGTFRSRAAAERHERAVQ
                     FFKRRG"
     gene            11187..>11412
                     /locus_tag="VFX87_10775"
     CDS             11187..>11412
                     /locus_tag="VFX87_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX4995447.1"
                     /translation="MIAAIEAVTIPRESGRTVPGLWRLPAGARSVLVLAHGAGAGMTH
                     KAMAAIADGLAARGIATLRYQFPYMERGSKR"
BASE COUNT         1719 a         3884 c         4237 g         1572 t
ORIGIN      
        1 ttggcgacga tgaagaccac cagcagcggc agcgcgctga gcgccgcccc cgccatgatg
       61 ggcccgtagg actccgagct gtaggcgcta cggagcaggg cgagcgcgac cggcagcggg
      121 aacatctccg gcttgttgac cgcgatgagc ggccacagga agtcgttcca cgccgagcgg
      181 aacgcgaaga tcgcgagcgc ggccagcacc ggctgcgaga gcggcaggat gatgcgggag
      241 aagacctgcc agggccccgc gccgtcgatg agggcggcgt cctccagctc gcgcggcatg
      301 gtgaggaaga actggcgcag cagaaagacc ccgaacgcga agagcatcgc cgcctccggc
      361 acgatcaggc cctggtaggt gctgagccag ccgagccgct gggtcagcac gtactggggc
      421 accagcagca ccgcgttcgg gatcatcagg ctgccgacca gcaccacgaa gacgaggtcg
      481 cggccgggga agcgcatgcg ggccagcggg taggcggcca gcgcgctggt cgcgaggacc
      541 agaaccaccg agccggttgc gacgaccagg ctgttgacga aggcgcggcc gatacgcgcg
      601 gtgcgcgagc tgcccagcag cacctcgtgg tagtgggcgc ccgtcggcgg ccacgggatc
      661 cactcgggag gcagccggat gatgtcgggc gcgggcttga gcgaggtcac caccacccag
      721 gcgatcggcg agagccagag cacggccagg gcgagcaggg ccaccgtgag ggccacgctc
      781 gagcggtcga cggggcggac gccgcgggcc accgctcagt actccgtgtg gccgcgcagg
      841 agacggaact gcagcaccga gaaggcgagg atgaccgcga agagcaccca ggccatcgcg
      901 gaggcggcgc cgaactggaa gaagttctgg aaggcggcct cgtagaggtg ctggaccacg
      961 gtgcgggtgg cgtcacccgg gccgccgccg gtgagcacga agacctggcc gaagagctgg
     1021 aacgagccga tcacgtgagt gaccaccacg aagagcagga ccgggcggag cagggggagg
     1081 gtgacggccc agaaggtgcg ccagccggag gcgccgtcga gcgcggccgc ctcgtagagg
     1141 tcgcggggga tgtcctgcag cccggccagg aggggtcagc cctccgcggc gagcgtgcgg
     1201 aaggctcgct cggtggcctc gagagtccgc gtgacgtctg cggtggtgtg ggcggccgag
     1261 acgaaccagt tgtgggtggg gtgcgcgtag gccccgccct cggtcacgag cgagcaccac
     1321 cggcgggtgc gccggcgctc gggatcgtcg gccaggcgga tcaccggcat ggcgggctgg
     1381 ccgaccggca tcagcgggat gcggtggcgc cgcgcctgct cggtgaggcc gtcgcacagg
     1441 agcgtgccga tgcgctggat gcgcgcgatc gcgtcggtgc gcgcgaggac gtcgagggtg
     1501 gccagggcgg cggcgaaggc gccgctcgcg aagaagaacg atccggtgaa gtagatgcgt
     1561 tcggccgctc cgcggaggcc ctcgcgcgcc agcccggccg agagggcgtg gccgttggcg
     1621 atcgccttgg agtagcagat gaggtcgggg gccgccccga agtgctcgca ggagccgccg
     1681 aggttgaacc ggaagcccgc gcgcacgtcg tccatcgcga acagcgcgcc gtgggcgtcg
     1741 cagagcgcgc ggagccccgc gaagaagccg ggcgcgggag cggtgccggt caggtggtcg
     1801 aacggggccg tcatgaccac cgcgatctcg ccggggtgcg cggcgaagag ggcgcccagc
     1861 tcgtcgaggt cgttccatgc gaactcgagc agatcgtcgc ggtagtgcgc gggcacgccg
     1921 gggaggtggg ggagggccca ggggcgcgcc gcgtggtagg cgccccgcac gcgcgcgatg
     1981 cggggccggc cggtatgcgc gcgcgccacg tccaccgccc agcttgtcgc gtccgcgccg
     2041 ttcttgccga agaccgcgaa gtccgcgcgc ggggtgaggg ccaccagccg ctccgccagc
     2101 tccaccatgc gggggcccgg cagggggagg cagtcgccgc ccgcgagctg ctcggccacc
     2161 gcggcctcca ccgcggggtg gccgtagccg aggagcatcg gtccgtaggc gcacatccag
     2221 tccacgtact cgttgccgtc cacgtcccag acgcgggagc gctcggcgcg ggcgagaaag
     2281 cgaggatggc cctcgcccag ggcggccccg ttctggtggc cgtagacgcc gcccggcatg
     2341 acgcgccggg cgcgctccca gagcgcgtgg ctcgcggcga ggcgcggggg cggggtctcc
     2401 atggccgggc atgatagcac ccgggcccgg gcctcagcgt ccgaggccca gcgccgtcac
     2461 ggttgcggga gttctacagg tgcgggagaa cggtggaggc gaagagccag gtgccttcgg
     2521 gccggggcac gtccgcgaag tttacggtga ggcggcgggc gccctgctcg acgatgccca
     2581 gcacggtctc ggtcacctgg gcgggggtgc cgacgatctg gatgtcgttg agcgagcgga
     2641 tccagggcgc ctgcttcagg cgctcgacct cgcgctcgct ctccgcgatc aggatgccga
     2701 cgcggaccac ctgctcgatc gcgtcgtagt cgcggcccac gtcgcggcag tgggccttga
     2761 gcacctcctg cttgtgcgcg taggcctccg gccctttcca cacgtagttc caccagtcgg
     2821 cgtgccgggc gaccacgcgc agcaggtact tctcgccgga gccgccgacc atcaccgggg
     2881 gcaccgggtt cggacgcggc tcgcagtagg cgcccgagat ctgatagtgc tcgccgtggt
     2941 actcggccgg cgcgcccgcc cacatgcgcc ggatcagctc gatggcctcc gcgagctggg
     3001 cgatgcgcac ccgggcgggc gggtagggcc acccgtaggt gcggtactct tcctcgttcc
     3061 agcccgcccc gatgcccagg atgacccgac cgcccgagag cgcctgcgcg gtcgccgcca
     3121 tcttggccag gtgggccggg ttgcggaagc tgttgcacag cacctcgtgg ccgcagcgct
     3181 tgtcgggata gcgggcgagc gcccaggtga gcagcgtcca gccctccgcg accttgttgg
     3241 cgccgtactg cacgtggtcc ggcatccaga ggatatcgaa gggtgccacg gtccggtcga
     3301 agtaggggaa ggtgtcctcg accagccgcg accagatgtt gagcccgacg ggaaccgcca
     3361 tggcgcggcg gccgctagcc gcggaagtgg cggcggtcgc ccgcgcggag ccagaggcgc
     3421 acgagatgtc gccgctcctc cggacgctcg gcgtcctggt acggcgtgcg gccgtgcacg
     3481 atcacgcagt tgttcagcac ctggatctgg ccgcgctcga cccagagatc gaggcgatgg
     3541 cccgggtcgc tgaggaacgc gtccatcagg cgcaccgcct ccgccccgtc cgcgtcgagc
     3601 gcgcggccgg tcttctcgta gccgcgcacg atgtgccggg cgttgaagcg cgtgcgcagg
     3661 cggccgtccc aggcgaacac gggccggaag ttggtggcgg ccgcgtcggc cgcctggtac
     3721 tcctgatgat cgcggtagaa gacctcgtac aggcggcgca gcagcgcggg gtgctcggcc
     3781 aggaaataat tgtgggcggc ggccgcgctc gacacgatgc tcatgccgcc ggcccgggcg
     3841 ggccgcaggc agaagaggct cacgtagttc ggcggcgcct cgcccatgct gctgtcggtg
     3901 tgcatctcga gcccttccgg agtcagggcg gcccgcgtgt cctgcgtgta ctcctgcccg
     3961 tcgtggcgca cgtcgtagat gacggtgccc ttccactcct gcgcgaccgg aggctcgagg
     4021 aagctcatca ggagccagta gagcgcgacc gcctcgtcgc ggctcatggc gtccaccggg
     4081 agccggtcga ggatcacgaa ggccgggccc tcgtcgagct ggcgccgcac ctcgcgcatc
     4141 caccgcgcgg tcgccgcgag cggaaagtgc tcgggcagca gcaggagcgt cggcaccggc
     4201 gcccggcgca gctcggcgag ggcgccctca agttcgctca gcaccgcggc gggaatcggg
     4261 atcaggtagc ggtcggcggg cagcgtgggg ccgtgccagg ccgcgggctc cgggatgatg
     4321 gcggagagca tggcgggcgc ctcggtggtc gtcacgggtc cgtattctag cagcccgggg
     4381 tggcgcgccg ggtctttacg tggcccgggc ccggcgttag catggcgccc gcgaaggggc
     4441 ggaccgtggg agtccgcgtg gagaagaacc ccgcagcacc cagagggccc aaggggagga
     4501 tcgccatgat aggagcctcg atgacgcggc ggttcgtctg gctgatcgtc gccgtcaccg
     4561 gtctcgcgct cgtcgcgtgg ccggccattg cccagcagtc cgccacgccg ccggccgagc
     4621 ctccgaagca ggaggaggtg cccttctggg cgatcggccg acccaaggcc gggccgggcg
     4681 cgcagatggc gccggtgccc gcgttcccga ttccgacgcc cgccgacaag ctgccgatcg
     4741 gcaagatgaa ggtcccgcct ggattcaagg tcgagctgtt cgcggccgac gtcttcgacg
     4801 cgcgcgggct gcggcaaggg gacaagggca cggtcttcgt cagctcgctg ttcggagccg
     4861 gcaagatcta cgccctcgtc gacaagggtg gtaggcgtga agtcaaggtc atcgccgaga
     4921 agctgatgct cccgaacggg atcgagttcc acaagggctc gctctacgtg gccacgccga
     4981 aggacatcac ccgctacgac gacatcgagg ccaagctcga caatccgccg gcgccggtga
     5041 aggtctacga ccagctcccg ggcgacgtgc cgcacggctg gaagttcatc aagatcgggc
     5101 ccgacgacaa gctctacgtg ccggtgggcg cgaactgcaa catctgcgag cccgaccccg
     5161 agaagtatgc gcagatccgc cgcatcaacc tcgacggcag cggcatggag atcgtggccc
     5221 gcggcgtgcg caacacggtg ggcttcgact tccacccgaa gaccggcgag ctctggttca
     5281 ccgacaacca gcgcgactgg ctctcggagg acctgcccaa cgacgagctg aaccgggtca
     5341 ccaagccggg ccagcaacac ttcggctttc cttactgcca ccagggcgac ttcgccgacc
     5401 cgcagctcgg ctggggcaag gcctgcgacg acttcgtgaa gccggtcctg ctgctggggc
     5461 cgcacgcggc cccgctcggc atgcggttct acaccggcag gatgttcccg ccgaagtacc
     5521 acaacgccat cttcatcgcg cgccacgggc cgtggaaccg caccaagaag tacgccgccg
     5581 acgtggtggc ggtgttcctc aatcccaacg gcaccgtgaa gtccatggag cccttcctca
     5641 ccggtctcgt cgagaacaac gcctatctcg gccgtccggt ggacgtgctc gtgctgaagg
     5701 acggctcgtt gctcgtctcg gacgaccacg cgggcgcggt ctaccggatc agctacaaga
     5761 agtgacggcc tccggcgggc cgggggcggt gatcccaccg cccccggccc gcgtcgtctt
     5821 cgtggccgcg ctcgtcctga tggccccgct gctgccggct gcacgggcca cggctcagtc
     5881 ggtggacgag cgcttcacgg tgtgcctcgc ctgccacggc gcggatgggc ggtcccgcat
     5941 cccggagact ccgtcgctcg gcgggcagcc tgcgttcttc gtgatcgcgc agctcttcct
     6001 gttccgcgag ggccgccggg acaacgcgcc catggtcgcg gcggccaagg gactcacgaa
     6061 cagcgacctg accgcctttg ccgagcgcgt gaccaggctg ccgccgcctc cgccacccga
     6121 ggacgcgccc gatcccgccc gctttgcgcg gggccggtcg ctgaccctgc gccacccctg
     6181 cggggtctgc cacaacccgg acttctcggg ccgcgagcag atgccgcgcc tggccaacca
     6241 gcgcgaggac tacctgctga agtccatgcg cgagtacaag agcggggcgc ggctgggcta
     6301 tggcggggcg atggcgcagg agctggccgg gctcaccgat cgggacctga tcgacctcgc
     6361 tcactttctc tcgcaccaga gcgtaacgcg ctgaaatggt gcgcggaacc tgggtcgaga
     6421 gtccacgtct tcgacgcgag tgaggccggt caatgtcccg gtctggagac agccgcgcgg
     6481 agcgcggccc agacgcgcgc cggcgacatg ggcatgtcga gctcgcgcac gccgcggccg
     6541 gccagcgcgt cgagcaccgc gttgaccgcg gacggcaggg cgcccgcgac gcccgattcg
     6601 cccgcgccct tcacgcccag gggattggtg gcgcagggga caccgtggtg gccgacgcgc
     6661 agcgcgggga ggtcgtcggc gcgcggcagc atgtagtcca tgaacgaggc ggtgaggagc
     6721 tggccgtgct cgtcgtagat cacgtgctcg cccaggacct ggcccaggcc ctgggcgatg
     6781 ccgccgtgga tctggccctc gacgatggtc tcgtgcacga tgcgccccac gtcatccacc
     6841 gccgcgtagc ccaccaccgc caccgcgccg gtctcgggat cgatctcgac ctcggtgacg
     6901 tggcagccgt tggggaagct catctggggc gagacgaact tggcggtgct gtcgaggcca
     6961 ccctgcagat cgggcgccag atccgggagg gcgcgggcgc gcacggccag gtcgagcagc
     7021 ccgatggcgc gatcggtgcc cttcacgcgg aagcggccat tggcgaactc cacgtcgggg
     7081 acggcggcct cgaagacgtg cgccgccagg cggcggcctt tctcgatgga cgtgtcgcag
     7141 gccatccagg ccgcactgcc cgccatcatc atggagcgcg aggcgacgct cggcgtgccg
     7201 gccggcacca ggtcgctgtc gccctgcacg agccgcaccg cgcccgggtc gatgccgagg
     7261 cgcgccgcga tcaggaaggg aaaggtggaa aggtggccct ggcccatggg ctgggccccg
     7321 gtgtgcagcg ccacggtgcc gtcgtcggcg aagcgcagct ccaccacctc gtcgaggatg
     7381 ccgccggcaa cctccaggaa gcaggcaaga ccgatgccgc gcagccgccc ggcgcgctcc
     7441 gaggtcgcgc ggcgctgcgg gaacccctgc cagtcggcta gggccagcgc ccggtccatg
     7501 atcgtctcga actcgccgct gtcgtagatc gggccgttgg gcgtgcgata gggcatggcg
     7561 gcggccggga tcaggttgcg ccgccgcagc gccacgcggt ccatcccggt gtcggcggcc
     7621 gcctgatcga tcaggcgctc gatgaggtag agcgcctccg gccgtccggc cccgcgatac
     7681 ggtccgagcg gcgccgcgtt ggtgaacccg agcgtgacgt cgatggcgat ggccgggatg
     7741 cggtacacgc tcgacaggca gttcttggtg ttgttggtcc cgaataccgc cgcgaacgca
     7801 gtgacgtacg cgccgatgcc cacccgggta cgcacccgca gggccaggaa gcgaccggtc
     7861 gcgtcgaggg cgagctcccc ctcgagcatg ccgtcgcggc cgtgggtgtc ggccaggaag
     7921 ctctcgacgc gggtcgcgcg ccacttgacg ggccggccca cccggcgcgc cgcgaagagc
     7981 agcgccgcgt actccgggta ggcctgcacc ttcatgccga agccgccgcc cacgtcgggc
     8041 gtgaccacgc gaagctgctg cggcgggatc ttgaacacgc cttccgcgag gagccggcgc
     8101 accaccgcga cgccctgggt ggaggcggtg agcgtgtagc gctgactggc ctcgtcccac
     8161 tggccgatgg cggcgcgcgg ctcgagcgcg gagggagcga gccgcgtgtc gtgcaggcgc
     8221 acccgtgcaa cgtgcgcggc ttcgcggatc gccgcgtcca ctgcggcggc gtccccgtcc
     8281 gtccagtcga aggcgacgtt ccgcgccgcg ccgggccaga tcggatgcgc ggaggcggcc
     8341 agcgcgcggt ccacgtcggc ggccgcgggc agcacgtcga gatcgaggcc cacgagagcg
     8401 gcggcgtcgg ccgcctgggc gggagtctcg gcgaccacga tggcgatcgc ctcgcccacg
     8461 tagcgcacgc ggtccaccgc gagcggagga atgggagcca ccgccagggg cttgccgccg
     8521 cggccgggga acgcggcggc gggcgggatc gcgcccaggc ccgccgcggc cgcatcgcgc
     8581 ccggtgagga ccgcgagcac gccgggcaag gcgagcgccc ggcgggtgtc gacggcgcgg
     8641 atgatcccgt gggccgcggt ggagcgggcg aacgcggcgt ggacctggcc gggcaggtcc
     8701 acgtcgtcgg tgaagcggcc gcggccggtg aggagcggac cgtcctcgct acgccgcccg
     8761 ccggccgcgg agccgaagcg cagcgaggac tcgggagcgg tcaattccgg ccagtcaatc
     8821 ccggaaagac gagtccgtac gatccagccg gcgcaggagg cccggccaga cgagccgacc
     8881 ctggccaccg ttgcggcgct ggttcgcgac cgcgttggcg tagcccctgt ccatgacttc
     8941 cttgggcacc gagatcagct cgccgccccc cgactgggcg cgcacctgga tctggcagga
     9001 ggcctcgagg tagtacatgt tcacgaaggc atcggccacc gtctcgccca ccgtgagcag
     9061 cccgtggttg cgcaggatca ggcagcgatt gtcgccgaga tccgccacca ggcgcggctt
     9121 ctcgtccgcg tcgagcgcgg ggccctcgaa gtcgtgatag cccacgctgt tgagcacgaa
     9181 cagcgagtgc tgcgagatcg gcagcagccc ggcccgctgg gtggagaccg cgacgccgtt
     9241 cgcggtgtgg gtatgcagca cgcacttggc gtcgtggcgg gcgccgtgga tggcgctgtg
     9301 gatgatgaac ccggccgcgt tcacgttgaa cggcgagttc tcgagcttcc tgccctcctg
     9361 gtcgatcttg accaggctag acgcggtgat ctcgtcgaag aagaggccgt agggattgat
     9421 caggaagtgg tcctcggtgc ccgggacgcg ggccgagatg tgggtgaaga ccaggtcctc
     9481 ccagccgtag cgcgacacca ggcggtaggc ggcggccagg tccacccgga cggcccactc
     9541 ctcgtccgat acctttccct tgagcgacgg aatctcgagc gcggtggcgg tcatggggtc
     9601 ctttcgctca gcggaggttg tagagcttga ccacgttgtc gtaggtgatc ttgcgctgca
     9661 cgcgctcggg caggttcccg aggttcttct cgagatgccg gcgcgagtcg ggccacacgc
     9721 agtccgggtg cgggtagtcc gcgccccaga tgatgttgtc ctcgcccacc agcgggatga
     9781 tcggctcgag gaacatatct tgttgatagg tcacgtagcc ctgccgcttg aagtagtcgc
     9841 tcggcttcat ggagaagccc aggccccggg cccggtcctc gtattcggtg tcgaagcggt
     9901 cgaacacgta gggcagccac gtcaccccgg actcgcccag cacgaacttg aagtccgggt
     9961 acttttcgca ggcgccggag gtgatgaggg agaccagcac ctccatggtg tcgagctgga
    10021 agagcgagga gcgcacgagc cgccactgga gcatgtactg ctgttccatc gcgggcgagt
    10081 cgggcgcgcg cacggccttg aagccggtcg aatggaaggc gatcgggaag tggcactcgg
    10141 ccgccgcctc ccagagcggg taccactcgt gctggtagag cgggatgctc atgctcttga
    10201 agccgagatc gccgcccttg aggcccatct tggcgcagcg gcgcacctcg gccgcggcca
    10261 cctgcgggtc gttgttgggc acgatcgcca gcgggtagat gcgattgggg tccgacttct
    10321 tcgcgaagtc ggcggcccag tcgttgtaga tcgcgttggc ccaggcccgg cgatcgccgc
    10381 tcgcgatcat gtcgttgatc atcaggcagc cgtagaccac ctcggcgtcg agcccgtcgc
    10441 ggtccaggtc ggccagccgc agctcgggcg tggtgggccg gggcttggcg cccgcgcggt
    10501 gatcccactc gaagccgcac gaggccatct cgtcgatccg ggcgaaggag ccgggcgtgc
    10561 gcttgaggaa gccgggtccc acgccgttcc acatcccctt gtcctgcgct tccacgaacc
    10621 agtggcagcc gtcctccagc tcctccacgc gcggcacgag cagcttccac ttcgacggcg
    10681 cctggctgga ccagaggtcg ggcgggcaat aggtgaggtc gatgtggttg tcgccggaga
    10741 tgagccgctc tagcatgacg ccctccttgg ccgggtgggg agctgtctcg tcgcggtgat
    10801 cctactccgg aagcccgcgc caggacaaga gactgccccg ggccagggcg ggacgcggcg
    10861 acccgggcac ctccggggtg gcgggaacct gcctagcgag gcgggtcttg ctcaccctcg
    10921 gcgcttgaag aactgcaccg cgcgctcgtg gcgctcggcc gccgcgcgag agcgaaacgt
    10981 ccccaggttg cgccgccgtc cggtgcgcgg atccttcttg cgcgaataca gccggtactg
    11041 gcctgacttg agcttgcgaa tcatgcggat ctctccaggc gggctcgcgg tcgccgcgaa
    11101 acccgagccg ccggattcaa gacgacgtgc gcggcagggt caagcgaacc ggcgccccga
    11161 gcccatgtgc taccctccgt gctccgatga tcgcagccat cgaagcggtg acgatcccgc
    11221 gcgagtcggg acgaaccgtg cccggactgt ggcgccttcc cgccggcgcg cgctcggtgc
    11281 tcgtgctcgc gcacggcgcg ggcgccggga tgacgcacaa ggcaatggcc gcgattgcgg
    11341 atgggctggc ggcgcgcgga atcgccacgc tgcgttatca gtttccctat atggaacgcg
    11401 gctcgaagcg cg
//
LOCUS       DASUFA010000287        12059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1315094, whole genome shotgun sequence.
ACCESSION   DASUFA010000287 DASUFA010000000
VERSION     DASUFA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12059
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315094"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..867
                     /locus_tag="VFX87_10780"
     CDS             187..867
                     /locus_tag="VFX87_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4995448.1"
                     /translation="MRILEVVGLTCRVGGVAVLDALSFTVEEGEIFALCGPAGAGKTV
                     GLRCLVGSLSFESGRLIYDGREITGTDPRERARQGLVRVGEISMPPWSLAVTEAITLA
                     RRWPRWGPFSAVVARWPGAEGRACDAALLERVGLATDADRRRDTLSLVEHARLEVAQA
                     LALAPRLLLLDDPLGRLPVEDRAAIAALITELRAAGVTIVLTEREPAVAEAVADRVAT
                     LDRGALVR"
     gene            864..1928
                     /locus_tag="VFX87_10785"
     CDS             864..1928
                     /locus_tag="VFX87_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995449.1"
                     /translation="MRAPGLAHLIGSVPLATSENVFRRLSAALGPVLSRMPDGETGER
                     RRWIYWQRTMLERHPAMEVDADAGLLELRQWDGSLLRRTDLLRFRPGVDPDSVLFPTG
                     YAEAARESWAVFTRLRREGVVPPGVRFQVCLPTAMSSACMYVSPRAHDDYLRVYERSL
                     LRALDDITAAIPHAELSIQWDICQEVLVFENYFPSRPADYKTRIFALLERLGAAVPAD
                     VELGYHLCYGSPADQHLVMPADAGVLAEITRAIVAAGRRPLDFIHLPVPRERDDAAYF
                     APLRGLRLPERTRLYLGLLHHDDAEGDRRRMDTARAAVGEGFGVATECGWGRGEPARL
                     DGLLESHRRAVDYLLAGSAR"
     gene            1925..2815
                     /locus_tag="VFX87_10790"
     CDS             1925..2815
                     /locus_tag="VFX87_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4995450.1"
                     /translation="MSAAMTDRMPGPASHTFFSQRLRLHYVDWGNPDKPPLLLLHGGR
                     DHCRNWDWTAAALRNDWHIIAPDLRGHGDSQWSTDGSYTMAGYIYDLAQLIHGQRLAP
                     VTIVAHSLGGNIALRYAGIYPETVARLVSIEGVGPSPRKLAERGAKTIAQRMDEWIRE
                     QRNLAGRLPRRYPSIEDAFRRMQEENPHLSAEQARHLTVHGVNQNEDGTYSWKFDNYV
                     RVFPPYDMRLRDIALLYSRITCPTLLLYGKESWSGNPADDGRLEPFRHASVIGFDRAG
                     HWLHHDRLHEFLKVLREFLR"
     gene            complement(2883..3770)
                     /locus_tag="VFX87_10795"
     CDS             complement(2883..3770)
                     /locus_tag="VFX87_10795"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX4995451.1"
                     /translation="MTQPISAADPYPRRRVAALDTEMAYVDVGAGDPVVFLHGNPTSS
                     YLWRNVIPHVAALGRCLAPDLVGMGDSGRAPAGSYRFVDHARYLDAWFDAIVPARPVT
                     LVVHDWGSALGFDWARRHPERLRGVAYMEALVRPVTWDEWPEPARKIFQAMRSAGGEE
                     MVLQKNVFVDRILPASVLRGLTEAEMAVYRRPYLEPGESRRPTLTWPRQIPIDGEPAD
                     VVAIVDAYAKWLAAAADLPKLFINADPGVILTGPQREFCRRWPNQQEITVRGSHFLQE
                     DSPDEIGEAVAAFVGRLPR"
     gene            3835..4695
                     /locus_tag="VFX87_10800"
     CDS             3835..4695
                     /locus_tag="VFX87_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4995452.1"
                     /translation="MIHALDHVVIAVRDLDAGIAAYQRLLGRPPCWRADAAGGGAETA
                     VFQLANVAAELIAPAGDGPDAARLRAVLDAQGEGLASLAFAVDDVERAHRRLSRLGLD
                     PEPAAAAESVDRATGARRRWRRTRAATAATHGVRVFLIEREAPPPAPPPAAPGGVTGL
                     DHVVIRTPDPERAAALYGARLGLDMRLDRSNPAWGARLMFFRCGDLIVELAHDLRAGV
                     SAGPDRLWGLSWRTDDSSAAIARLGGAGLDVSEARPGRKPGTHVFTVRNGTCGVPTLM
                     LQPSGPPAQP"
     gene            4727..6262
                     /locus_tag="VFX87_10805"
     CDS             4727..6262
                     /locus_tag="VFX87_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995453.1"
                     /translation="MTRSRLALALLVLAVSIWPAAAAPAAAQTRPEGEMRFAFYVTIS
                     PTWFDPGDVTGFITPFWTLWALHDALVKTMPGKPMAPSLAESWTVSADGKTYDFKLRP
                     NLKFHNGDPFTAEDVKFSFQRAKGYKILREKVRDIEVAGPLRVRIHLHEPWPDFMTFY
                     GTMVAGQGWIVPKKYVEQVGDDGFKKHPIGLGPYKFVSHTPGVELVLEAFEGYWRKAP
                     SVKRLVFKSVPEANTRYAMLKRGEVDLAYLLDVPQAQELKRDSNFKVAFSGGIATFYL
                     DYFDMWDPKSPWADRRVRLAASHALDRKALNEAETMGQSRLTGGFVPRNLEFALALDA
                     HAYDPAKAKQLLAEAGYPNGFDAGELHPFPPYTSMGEAAAGYLQAVGIRTRIRTMERA
                     AFISEWGAKKLRGVCLCITGVYGNAATRMSEYVPSTGAYAYGGWPDVDALYQQQARET
                     DRKKREALLHQIQRTLAERVRFGMIYDYVWPSGVGPRVAEPALLLIDPYPWAAPYEEL
                     RLR"
     gene            complement(6331..8655)
                     /locus_tag="VFX87_10810"
     CDS             complement(6331..8655)
                     /locus_tag="VFX87_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4995454.1"
                     /translation="MASKLLGAHVKRKEDPRLITGVSQYVADIAMPGMQHVAFVRSPH
                     AHARVRGIKSAAAAKLPGVVAVVTGQDLKAHFAPIPVATASAEGGAAGEIKLGRQHYP
                     LSLDRVRYVGEPVAAVIGLSPEAAMDAAALVEVDWEPLPAVADAFAAMADGAPQLFDD
                     APKNIEHTNTIKAGDPDAAFAKAHRVVKQRMNSQRLSGIPMETRAVLAAPDFASGGLT
                     VWATHQAPHVLRGGLADALRMPQNQIRVICPEVGGGFGVKFGTYPEDVTVAALARLRK
                     IPLRWIESRVEHMTGTTHGRAQMTDLEAAVDAEGRINGLRMHVVADIGAYPIFTFIPD
                     LTLMMGVGVYQVKNVELKSTCVFTNTTSVAAYRGAGRPEAAYYLERLVDCIAMDLGKP
                     PEDVRRVNFIPPSAFPYAAPTGQNYDSGEYARALAKSLEVSKYTALRAEQRKRIEAND
                     RKLLGIGMACYVEMCGFGPYESAMIRVEPSGTVTAYTGASAHGQGHETTFAQLIADYL
                     GVDFDQVVVRHGDTTGAPMGFGTGGSRSLVVGGSAMVKAAEKVQEHARRIAASDLEAA
                     FEDVVVTDGRYHVKGSPAKSLTLAKIAEKAYGEGLPAGMEPGLEATEYFRPPQLVYPF
                     GAHVAVVEVDRDTGRVTLRDFTSVDDCGVRVSPLLVDGQVHGGIAQGVAQSLLEEVVY
                     SADGQLVTGTLMDYAVPRAEDVPFFVTDQTVTPSPFNPLGAKGIGEAATIGSTPAVVN
                     AVVDALKPFGVRHLDMPLRPERVWRAMQGGSARA"
     gene            complement(8670..9251)
                     /locus_tag="VFX87_10815"
     CDS             complement(8670..9251)
                     /locus_tag="VFX87_10815"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX4995455.1"
                     /translation="MLSDDGLDLLFRNARTHKVWLDRPVPDDLLRRVYDLARLGPTSA
                     NCSPMRVLFLTSREAKERLRPALSPANVDKTMQAPVTAVVGHAVDFYDELPRLFPTAD
                     MRVHFAGKPEFAATTAFRNGSLQGAYLMLAARALGLDCGAMSGFDNAKVDAEFFPDGR
                     VKSNFLCNLGHGDASKLTPRGPRLSFDEACRIL"
     gene            9400..10779
                     /locus_tag="VFX87_10820"
     CDS             9400..10779
                     /locus_tag="VFX87_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX4995456.1"
                     /translation="MARKPADMTTKYLLSEKELPTRWYNIQADFKTPLPPPLHPGTGQ
                     PIGPQDLAPLFPMELIKQEVSQERWIEIPDEVRDILRLWRPTPLYRARRLEKALDTPA
                     RIYYKYEGVSPAGSHKPNTAIAQAYYNKQEKVARLATETGAGQWGSALSQACSFFGLQ
                     CKVYMVKVSYQQKPYRRILMETFGAQVVASPSPDTQAGKKVLEQDPSSPGSLGIAISE
                     AVEDAATRDDTKYSLGSVLNHVLLHQTVIGLETRKQLEKADDEADVIVGCIGGGSNFA
                     GFSFPFVADRLTGKNKKRRIVAIEPSACPSLTKGTYAYDFGDTVGLTPLVKMHTLGHT
                     FVPAPMHAGGLRYHGMAPLICKLYDEGLIEARAVPQLATFEAAVLFARAEGILPAPEA
                     AHAVRGAIDEALTAKKEGKRKTIVFSLSGHGFLDLSAYEAYLAGKLQDYEYPAEQVQE
                     ALRHLPQLK"
     gene            10859..12052
                     /locus_tag="VFX87_10825"
     CDS             10859..12052
                     /locus_tag="VFX87_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995457.1"
                     /translation="MSNTSPTALLLNVGHAMDHLFLLIFATAVSGIATQWGMVWQDLM
                     PYTVGAFVMFGLGSLPAGRLGDLWGRRVMMIIFFLGLGAAGLVVATAQRPWQLAVALT
                     LMGAFASIYHPVGIPMIVQHSTRPGFTIGLNGLAGNLGIAFAAVLTGFLVQQAGWRMA
                     FAVPSLLSIACGITFALVVPREDMAPAKKPKRQVDLPPGLMARIFFVMSLAAVSSSMI
                     FNFTTNGNNQLLAERLRGLVDNPATLGVLLAVVYAIASLAQIVVGKLIDRYPLKYVYL
                     PIVAAQVPLFALAAHSEGWALYALMLLFMVVVFGAIPFVDAMIVQYVDDRMRSRVAGM
                     RLAVSFGVSALAVYLLGPTVKAAGFTTLLLVMAGISSLTTLFVSLLPGRVPSSAPAAA
                     PAPAR"
BASE COUNT         1680 a         4464 c         4166 g         1749 t
ORIGIN      
        1 agaacgtggt ctacgcgatg tcccaccagg cggtgaccga cgtgtgggtc cacggccacc
       61 cggtggtgcg cggccagcgc ctggccacga tggaccaggc cggcctcatg gagcgcgtcc
      121 gcgcggtgac gttcgactgg aggctcgcgt gacggttcga ctggcggcgg cccgcgtgag
      181 gcccgcatga gaatactcga ggtcgtcggg ctcacctgcc gcgtgggcgg cgtcgcggtg
      241 ctggatgcgc tctcgttcac cgtggaggag ggcgagatct tcgcgctctg cggtcccgcc
      301 ggggcgggca agaccgtcgg cctgcgctgt ctggtcggct cgctctcctt cgagtcgggg
      361 cgcctcatct acgacggccg cgagatcacc gggacggatc cgcgcgagcg tgcccggcag
      421 gggctggtcc gggtcggcga gatctccatg cccccctggt ccctggccgt cacggaggcg
      481 atcaccctgg cccgccgctg gccgcgctgg ggcccgttca gcgcggtcgt ggcccgctgg
      541 cccggcgcgg aaggccgcgc gtgcgacgcc gcgctgctcg agcgcgtcgg gctcgccacc
      601 gacgcggacc ggcggcgcga caccctgtcg ctggtggagc acgcgcggct cgaggtggcc
      661 caggccctgg ccctcgcgcc gcgcctgctc ctcctcgacg atccgctcgg ccggctgccc
      721 gtggaggacc gcgcggccat cgccgcgttg atcaccgagc tgcgcgccgc cggcgtcacg
      781 atcgtgctga ccgagcggga gccggccgtc gcggaggcgg tggcggatcg cgtggccacc
      841 ctcgaccgcg gagccctggt ccgatgaggg cgccggggct cgcgcacctg atcggcagcg
      901 tcccgcttgc caccagcgag aacgtcttcc gccgtctctc cgccgcgctc ggccccgtgc
      961 tctcccgcat gcccgacggc gagaccgggg agcgccggcg ctggatctac tggcagcgga
     1021 ccatgctcga gcgccatccc gccatggagg tggacgcgga cgcgggactg ctcgagctgc
     1081 gccagtggga cggctccctg ctgcgccgca ccgatctcct gcgcttccgg ccgggcgtgg
     1141 atccggactc cgtcctgttc ccgaccggct acgcggaagc cgcgcgcgag tcgtgggccg
     1201 tcttcacgcg gctccgccgc gagggcgtgg tgccgccggg ggtgcggttc caggtctgcc
     1261 tgcccaccgc catgtcgagc gcctgcatgt acgtgagccc gcgcgcgcac gacgactacc
     1321 tgcgcgtcta cgagcgctcg ctgctccggg ccctcgacga catcacggcc gccatccccc
     1381 acgccgagct cagcatccag tgggacatct gccaggaggt gctggtgttc gagaactact
     1441 tcccgtctcg tcccgccgac tacaagactc ggatcttcgc gctgctcgag cgcctggggg
     1501 ccgcggtgcc cgccgacgtc gagctcggct atcacctctg ctacggcagc ccggccgacc
     1561 agcacctggt gatgccggcc gacgcgggcg tgctcgccga gatcacccgc gccatcgtgg
     1621 ccgcggggag gcggccgctc gacttcatcc acctgccggt accgcgcgag cgcgacgacg
     1681 ccgcgtactt cgcgcccctg cgcgggctgc gcctgcccga gcgcacgcgc ctctacctcg
     1741 ggctcctcca ccacgacgat gccgagggcg accggcggcg catggacacc gcccgcgccg
     1801 cggtgggcga gggcttcggc gtggccaccg agtgcggctg ggggcgcggc gagcccgcgc
     1861 ggctcgacgg cctgctcgag agccaccgcc gcgcggtgga ctacctgctc gcgggatccg
     1921 cgcgatgagc gccgccatga ccgaccgcat gccgggcccc gcgtcgcaca cgttcttctc
     1981 ccagcgcctc cgcctgcact atgtcgactg gggcaatccc gacaagccgc cgctcctcct
     2041 gctccacggc gggcgcgatc actgccgcaa ctgggactgg accgcggcgg cgctccgcaa
     2101 cgactggcac atcatcgcgc cggacctgcg cggccacggc gacagccagt ggtccaccga
     2161 cggcagctac acgatggccg gctacatcta cgacctcgcc cagctcatcc acggtcagcg
     2221 gctggccccg gtcacgatcg tggcccactc gctgggcggc aatatcgctc tgcgctacgc
     2281 cgggatctac ccggagacgg tggccaggct ggtgtccatc gagggcgtgg ggccgtcccc
     2341 ccgcaagctc gccgagcgcg gggccaagac gatcgcgcag cggatggacg agtggatccg
     2401 cgagcagcgc aacctggccg gccggctccc gcggcgctac ccctcgatcg aggacgcctt
     2461 ccggcgcatg caggaggaga acccgcatct ctccgccgag caggcgcggc acctcaccgt
     2521 ccacggggtg aaccagaacg aggacgggac gtacagctgg aagttcgaca actacgtgcg
     2581 cgtgttcccg ccctacgaca tgcgcctgcg ggacatcgcg ttgctctact cacgcatcac
     2641 ctgcccgacg ctgctgctct acggcaagga gagctggtcc ggcaatcccg ccgacgacgg
     2701 ccgcctcgag ccgttccgcc acgcgagcgt catcggcttc gaccgcgccg ggcactggct
     2761 gcaccacgac cgcctccacg agttcctgaa ggtcctccgc gagttccttc gatagaccac
     2821 ccctcacccc gaccccctcc cccgagggga gagggagagg gcagggtgag ggggcgcgag
     2881 gctcagcgcg gcagccgccc cacgaacgcg gccaccgcct cgccgatctc gtccggcgaa
     2941 tcctcctgga ggaagtgact cccccgcacc gtgatctcct gctggttcgg ccagcgccgg
     3001 cagaactcgc gctgcggtcc ggtgaggatg acgcccggat ccgcgttgat gaagagcttc
     3061 ggcaggtcgg cggccgccgc cagccatttc gcgtacgcgt ccacgatggc caccacgtcg
     3121 gcgggctcgc cgtcgatcgg gatctgccgg ggccaggtga gcgtcgggcg ccgcgactcg
     3181 ccgggctcca ggtacggccg gcggtagacc gccatctcgg cctcggtcag gccgcgcagc
     3241 acgctcgcgg gcaggatccg atccacgaac acgttcttct ggagcaccat ctcctcgccg
     3301 cccgctgacc gcatggcctg gaagatcttc cgcgccggct ccggccattc atcccacgtc
     3361 accggccgga cgagcgcctc catgtaggcc acgccgcgca gccgctcggg atggcgccgc
     3421 gcccagtcga agccgagcgc cgagccccag tcgtgcacga ccagcgtcac cgggcgcgcc
     3481 gggacgatcg cgtcgaacca cgcgtcgaga tagcgcgcgt ggtccacgaa gcgataggag
     3541 cccgcgggcg cgcggcccga gtcgcccatg cccaccagat ctggggccag acagcggccg
     3601 agcgccgcca cgtgcgggat cacgttgcgc cagaggtagg acgaggtcgg gttgccgtgc
     3661 aggaacacca ccggatcgcc cgcgcccacg tccacgtagg ccatctcggt gtcgagcgcc
     3721 gccacgcggc ggcgcggata cggatcggcg gcagaaatcg gctgtgtcat cgcctggatg
     3781 ctacacttcc ggcaccgtgc ccgccacctc ggctctgcgc gacatggcca tcgcatgatt
     3841 cacgcgctcg atcacgtggt catcgcggtg cgcgatctcg acgccgggat cgctgcctac
     3901 cagcgactgc tcggccgccc cccgtgctgg cgggcggacg cggccggcgg gggcgccgag
     3961 accgcggtct tccagctcgc caacgtcgcc gcggagctga tcgcgcccgc gggcgacggc
     4021 cccgacgcgg cgcgcctgcg cgccgtcctc gacgcccagg gcgaagggct cgcgagcctg
     4081 gccttcgcgg tggacgacgt cgagcgcgcc caccgtcggc tctcccggct cggcctcgat
     4141 cccgagccgg ccgccgccgc ggagagcgtg gaccgcgcga ccggcgcgcg ccgccgctgg
     4201 cgccgcaccc gcgccgcgac ggcggcgacc cacggcgtgc gcgtgttcct gatcgagcgc
     4261 gaggccccgc cgcccgcccc gccgcccgcc gcgccgggcg gcgtcaccgg cctcgaccac
     4321 gtggtcatcc gcacgcccga tcccgagcgg gccgccgcgc tctacggcgc gcgcctgggg
     4381 ctcgacatgc ggctcgaccg gtcgaatccc gcgtggggcg cgcgcctcat gttcttccgc
     4441 tgcggggatc tgatcgtgga gctggcccac gatctccgcg ccggggtctc cgccggaccg
     4501 gatcgcctct ggggtctctc ctggcggacc gacgactcgt cagcggcgat cgcccgcctc
     4561 ggcggcgccg gcctcgacgt ctccgaagcc cggcccggcc gcaagccggg cacgcacgtc
     4621 ttcacggtgc gcaacgggac ctgcggcgtc ccgacgctca tgctgcagcc gtccggaccg
     4681 cccgctcagc cctgaaccgc catcgtctgg agcacctgga ggcctcatga cccgctcccg
     4741 cctcgcgctc gcgctgctcg tcctcgccgt gtccatctgg cccgccgccg ccgcccccgc
     4801 ggccgcccag accaggccgg agggcgagat gcgcttcgcc ttctacgtca cgatctcccc
     4861 cacctggttc gacccgggcg acgtgaccgg cttcatcacc ccgttctgga cgctgtgggc
     4921 gctgcacgac gcgctggtca agaccatgcc cggcaagccg atggccccga gcctcgccga
     4981 gtcgtggacg gtgagcgcgg acggcaagac ctacgacttc aagctgcgcc ccaatctcaa
     5041 gttccacaac ggcgacccct tcaccgcgga ggacgtgaag ttcagcttcc agcgcgcgaa
     5101 gggctacaag atcctgaggg agaaggtccg cgacatcgag gtggccggcc ccctgcgcgt
     5161 gcgcatccac ctgcatgagc cgtggccgga cttcatgacc ttctacggca cgatggtcgc
     5221 gggccagggc tggatcgtgc cgaagaagta cgtcgagcag gtcggcgacg acggcttcaa
     5281 gaagcatccg atcggcctcg gcccctacaa gttcgtgagc cacacgccgg gcgtggagct
     5341 ggtcctcgag gccttcgagg gctactggcg aaaagccccc tccgtgaagc ggctcgtgtt
     5401 caagagcgtg ccggaagcca acacgcgcta cgccatgctc aagcgcggcg aagtggacct
     5461 cgcctatctt ctggatgtcc cgcaggctca ggagctcaag cgcgactcga acttcaaggt
     5521 cgccttctcg ggcggcatcg cgaccttcta cctggactac ttcgacatgt gggacccgaa
     5581 gtcgccttgg gccgatcggc gcgtgcgcct cgcggcgagc cacgcgctgg accggaaggc
     5641 gctgaacgag gccgagacca tgggccagtc ccgcctgacc ggcggcttcg tgccgcggaa
     5701 cctcgagttc gcgctggccc tcgacgccca cgcctacgac cctgccaaag ccaagcagct
     5761 cctcgccgag gcgggctacc cgaacggctt cgacgcgggc gagctgcacc cgttcccgcc
     5821 ctacaccagc atgggcgagg cggccgccgg gtacctgcag gcggtgggca tccggaccag
     5881 gatccgcacc atggagcggg ccgccttcat ctcggagtgg ggcgcgaaga agctccgcgg
     5941 ggtctgcctc tgcatcaccg gggtctacgg caacgcggcg acgcggatgt cggagtacgt
     6001 gccgagcacc ggggcctacg cctacggcgg gtggccggac gtggacgcgc tgtaccagca
     6061 gcaggcgcgc gagaccgatc ggaagaagcg cgaggcgctg ctccaccaga tacagcggac
     6121 cctggcggag cgggtgcgct tcgggatgat ctacgactac gtgtggccca gcggcgtcgg
     6181 cccccgggtc gccgagcctg cgctcctgct catcgacccg tacccgtggg ccgcgcccta
     6241 cgaggagcta cgccttcgct agaagagccg tgcctcccca tccccctctc ccctcagggg
     6301 agagggcagg gtgaggggcg agttgcgggg ctacgccctc gccgacccgc cctgcatcgc
     6361 ccgccacacc cgctccggcc ggagcggcat atccaggtgg cgcacgccga acggcttcaa
     6421 cgcgtccacc accgcgttga ccacggcggg cgtcgagccg atcgtcgcgg cctcgccgat
     6481 gcccttcgcc ccgagcgggt tgaacggcga gggcgtgacg gtctggtccg tcacgaagaa
     6541 cggtacgtcc tccgcgcgcg ggaccgcgta gtccatcagc gtgccggtga cgagctgacc
     6601 gtccgcgctg tagaccactt cctccaggag tgactgggcc acgccctggg cgatgccgcc
     6661 gtgcacctgg ccgtccacca ggagcgggct gacgcggacg ccgcagtcgt ccaccgaggt
     6721 gaagtcgcgc agcgtgaccc ggccggtgtc ccggtccacc tccaccaccg ccacgtgcgc
     6781 gccgaacgga tagacgagct gcggcgggcg gaagtactcg gtggcctcga gccccggctc
     6841 catgccggcg ggcaggccct cgccgtaggc cttctcggcg atcttggcca gggtcaggct
     6901 cttggccggc gagcccttga cgtgatagcg cccgtcggtg accacgacgt cctcgaacgc
     6961 ggcctcgaga tccgaggccg cgatgcggcg ggcgtgctcc tgcaccttct cggccgcctt
     7021 caccatggcc gagccgccga cgaccaggct gcggctgccg ccggtgccga aacccatcgg
     7081 ggcgccggtg gtgtcgccgt ggcgcaccac cacctggtcg aagtccacgc ccaggtaatc
     7141 ggcgatgagc tgggcgaacg tggtctcgtg accctgcccg tgggcggagg ccccggtgta
     7201 ggcggtcacc gtcccgctcg gctccacccg gatcatcgcg ctctcgtagg ggccgaagcc
     7261 gcacatctcg acgtagcagg ccatgccgat gccgagcagc ttccgatcgt tggcctcgat
     7321 ccgcttgcgc tgctcggcgc ggagcgcggt gtacttcgac acctcgaggc tcttggcgag
     7381 cgcgcgcgcg tactcgccgc tgtcgtagtt ctgcccggtg ggcgcggcat aggggaacgc
     7441 gctcggcggg atgaagttga cccggcggac gtcctcgggc ggcttgccca ggtccatcgc
     7501 gatgcagtcc accagccgct cgagatagta ggcggcctcg ggccggcccg cgccgcggta
     7561 ggcggccacc gaggtggtgt tggtgaacac gcacgtgctc ttgagctcca cgttcttcac
     7621 ctggtacacg cccaccccca tcatcagggt gaggtcgggg atgaacgtga agatcgggta
     7681 ggcgccgatg tcggccacca cgtgcatgcg gaggccgttg atgcggccct cggcgtccac
     7741 cgccgcctcc agatcggtca tctgggcgcg cccgtgcgtg gtgccggtca tgtgctcgac
     7801 ccggctctcg atccaccgca gcgggatctt ccgcagccgc gcgagtgcgg cgaccgtcac
     7861 gtcttccggg tacgtgccga acttcacgcc gaagccgccg ccgacctccg ggcagatcac
     7921 ccggatctgg ttctggggca tgcggagcgc gtcggccagc ccgccgcgga gcacgtgggg
     7981 cgcctggtgg gtggcccaca ccgtgaggcc gccgctcgcg aagtcgggcg cggccagcac
     8041 cgcgcgggtc tccatcggga tgccggagag ccgctggctg ttcatgcgct gcttgaccac
     8101 ccggtgcgcc ttcgcgaagg cggcgtcggg atccccggcc ttgatggtgt tggtgtgctc
     8161 gatgttcttg ggcgcgtcgt cgaagagctg gggcgccccg tccgccatcg cggcgaaggc
     8221 gtcggcgacc gcgggcaacg gctcccagtc cacctcgacg agggcggccg cgtccatcgc
     8281 ggcctcgggc gagaggccga tcaccgcggc caccggctcg cccacgtagc gcacgcggtc
     8341 cagcgagagc gggtagtgct ggcgcccgag cttgatctcg cccgcggcgc cgccctcggc
     8401 ggacgcggtc gcgaccggga tcggcgcgaa gtgcgccttg agatcctggc cggtcaccac
     8461 cgcgaccacg ccggggagct tcgcggcggc ggcgctcttg atcccgcgga cccgggcgtg
     8521 cgcgtgcggg ctgcggacga aggcgacgtg ctgcatgccc ggcatcgcga tgtcggccac
     8581 gtactgcgac acgccggtga tcaggcgcgg gtcctccttg cgcttgacgt gggctcccag
     8641 gagcttggat gccatgatcg gatcgcctcc tacaggatgc ggcaggcctc gtcgaacgac
     8701 aggcgcggcc cgcgcggagt gagcttgctc gcgtcaccgt gacccagatt gcacaggaag
     8761 ttcgacttca cgcgcccgtc cggaaagaac tcggcatcca ccttggcgtt gtcgaagccg
     8821 gacatcgcgc cgcagtcgag gccgagcgcg cgcgcggcca gcatgagata cgcgccctgg
     8881 aggctgccgt tgcggaacgc ggtcgtggcg gcgaactcgg gcttccccgc gaagtgcacg
     8941 cgcatgtcgg cggtcgggaa caggcgcggc agctcgtcgt agaagtccac cgcgtggccg
     9001 acgacggcgg tgaccggcgc ctgcatcgtc ttgtccacgt tggccgggct cagcgcgggg
     9061 cgcagccgct ccttggcctc gcgcgaggtc aggaacagca cgcgcatcgg gctgcagttg
     9121 gcgctggtgg ggccgagccg cgccaggtcg tagacccggc gcaggagatc gtccggcacc
     9181 ggccggtcga gccagacctt gtgggtgcgc gcgttgcgga agagcaggtc gaggccgtcg
     9241 tcgctgagca tggagggagt atcacggcac cgccgcgcgg cgtcaagccg ctgcgacgcg
     9301 ctcgagaggc cgcccgcttg acggccttcc cgggcaccct gtactgtggg gcccgattcg
     9361 cgatgcgccg accgcgcggc gcaccaaagg aggtcgccca tggctcgcaa gccggccgac
     9421 atgaccacca agtacctgct ctccgagaag gagctgccca cccgctggta caacatccag
     9481 gccgacttca agacgccgct gccgccgccg ctgcatccgg gcaccggcca gccgatcggg
     9541 ccgcaggatc tcgcgccgct cttcccgatg gagctgatca agcaggaggt cagccaggag
     9601 cggtggatcg agatccccga cgaggtgcgc gacatcctgc gcctgtggcg cccgaccccg
     9661 ctctaccgcg cgcggcggct cgagaaggcg ctcgatacgc cggcccgcat ctactacaag
     9721 tacgagggcg tgagcccggc cggcagccac aagccgaaca ccgcgatcgc gcaggcctac
     9781 tacaacaagc aggagaaggt cgcgcgcctg gccaccgaga ccggcgcggg ccagtggggc
     9841 tccgcgctct cccaggcctg ctcgttcttc ggcctgcagt gcaaggtgta catggtgaag
     9901 gtgtcgtacc agcagaagcc ctaccgccgc atcctcatgg agacgttcgg cgcgcaggtc
     9961 gtggcgagcc cgagccccga cacccaggcg ggcaagaagg tgctcgagca ggatccgagc
    10021 tcgccgggca gcctcggcat cgcgatcagc gaggcggtgg aggacgcggc gacccgcgac
    10081 gacacgaagt actcgctcgg cagcgtgctc aaccacgtgc tcctgcacca gaccgtgatc
    10141 gggctcgaga cgcgcaagca gctcgagaag gcggacgacg aggccgacgt catcgtgggc
    10201 tgcatcggcg ggggctccaa tttcgcgggc ttctccttcc cgttcgtggc cgaccggctc
    10261 accggcaaga acaagaagcg gcggatcgtg gccatcgagc ccagcgcctg cccgagcctc
    10321 accaagggca cgtacgccta cgacttcggc gacacggtgg gcctgacgcc gctcgtgaag
    10381 atgcacacgc tcggccacac cttcgtcccc gcgccgatgc acgcgggggg cctgcgctac
    10441 cacggcatgg ccccgctcat ctgcaagctc tacgacgagg gcctgatcga ggcgcgcgcg
    10501 gtgccccagc tcgccacctt cgaggccgcg gtcctgttcg cgcgggccga agggatcctg
    10561 cccgcccccg aagcggcgca cgcggtgcgc ggcgccatcg acgaggcgct caccgcgaag
    10621 aaggaaggca agcgcaagac catcgtgttc agtctctcgg gccacgggtt cctcgacctc
    10681 tccgcctacg aggcgtatct ggccgggaag ctccaggact acgagtaccc cgcggagcag
    10741 gtgcaggagg cgctgcgcca cctgccccag ctcaaatgag ggcccgtggg gccgcgcggt
    10801 acggtatgct ctacgccaat cgcttctatc ccgcagcgta gcggagacca acggccccat
    10861 gtcgaacacg agccccaccg cgctcctcct gaacgtcggc cacgcgatgg accacctctt
    10921 cctgctgatc tttgccaccg cggtgtcggg gatcgcgacg cagtggggca tggtctggca
    10981 ggacctgatg ccctacacgg tgggcgcgtt cgtgatgttc gggctgggct cgctccccgc
    11041 gggccgcctg ggcgacctct ggggccggcg cgtcatgatg atcatcttct tcctggggct
    11101 cggcgcggcg gggctggtgg tggcgacggc ccagcgtccc tggcagctcg cggtcgcgct
    11161 caccctcatg ggcgcgttcg cctccatcta ccacccggtg ggcatcccga tgatcgtgca
    11221 gcactcgacg cgcccgggct tcaccatcgg gctcaacggg ctcgcgggca acctggggat
    11281 cgcgttcgcg gcggtcctga ccggcttcct ggtccagcag gcgggctggc gcatggcctt
    11341 cgcggtgccg tccctgctgt ccatcgcgtg cggcattacc ttcgcgctcg tggtcccgcg
    11401 ggaggacatg gcgcccgcca agaagcccaa gcgccaggtg gacctgccgc ccggactgat
    11461 ggcgcggatc ttcttcgtga tgtcgctcgc cgcggtctcg agcagcatga tcttcaactt
    11521 caccaccaac gggaacaacc agctcctggc cgagcggctc cgcggcctgg tggacaatcc
    11581 cgccaccctc ggcgtcctcc tcgcggtggt ctacgcgatc gcctcgctcg cccagatcgt
    11641 ggtggggaag ctgatcgatc gttacccgct caagtacgtc tacctgccca tcgtggccgc
    11701 gcaggtcccg ctcttcgccc tggcggccca ctcggagggc tgggcgctgt acgccctcat
    11761 gctcctcttc atggtggtcg tgttcggcgc catcccgttc gtcgacgcca tgatcgtgca
    11821 gtacgtggac gatcggatgc gctcccgggt ggccggcatg cgcctcgcgg tctccttcgg
    11881 ggtcagcgcg ctggcggtct acctgctggg gccgacggtc aaggcggcgg gcttcaccac
    11941 gttgctgctg gtgatggcgg gcatctcgtc cctcaccacg ctcttcgtct ccctgctgcc
    12001 cggccgcgtc ccgtcctccg cgcccgccgc cgcgcccgcg ccggcgcgct agcgcggcc
//
LOCUS       DASUFA010000288         4963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_638334, whole genome shotgun sequence.
ACCESSION   DASUFA010000288 DASUFA010000000
VERSION     DASUFA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638334"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFX87_10830"
     CDS             complement(<1..183)
                     /locus_tag="VFX87_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEX4995458.1"
                     /translation="MADVKSDRRQALELAITAIDRQFGKGAIMRLGQNGAFDEIAVIP
                     TGAMQLDVALGIGGIPR"
     gene            complement(358..936)
                     /gene="thpR"
                     /locus_tag="VFX87_10835"
     CDS             complement(358..936)
                     /gene="thpR"
                     /locus_tag="VFX87_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX4995459.1"
                     /translation="MIRAFVAVVLDDGVRAAIAETIGRLRPLGSAVAWVPPRNLHITL
                     HFLGDQSEERLEEAEAALADAAAASAPLDVTFHGIGAFPGLERPRILWVGLAQGALEA
                     RRLQARVTDALAARGFGKEERAWHPHLTIGRVHDERRWRREAGPPLRSALAQAATTTF
                     GTQHVTEVALMRSDLSPSGARYTVRRALSLGA"
     gene            complement(974..2224)
                     /locus_tag="VFX87_10840"
     CDS             complement(974..2224)
                     /locus_tag="VFX87_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX4995460.1"
                     /translation="MSGAWIVTVGAAQLGAADDPAGMMVARALLAEGVPVASRQVVDE
                     DETAVEEALRGAVEAAALVVVLAQPGGSSGEVVRRALSRLGGARLVLNDKLLASMEED
                     FARRGRAMPRRLDRLALLPQGALLWPAGGEPAWVLEVRRAAVAVLPTGSAMLASLVES
                     HLRPLARQRLGAGESTVLRTLHTTGLSPADAEERLGRWLGVEGAVTVSCVPVEGDVWV
                     QLRSRASSRSAAEAALVPLEREVREALGPDCWGKDEDTLEVVVGRLLLERRLTVSMAE
                     SCTGGLVGHRLTNIAGSSRYVERGVVVYSNQAKEQLLGVPEEMLRAHGAVSAPVAEAM
                     AAGICRISGSACGIAVTGIAGPDGGTPTKPVGTVFIAAAAPGADGPRIDVRRFRFAGG
                     REAVKWQSSQAALDMLRRALLARA"
     gene            complement(2232..2669)
                     /locus_tag="VFX87_10845"
     CDS             complement(2232..2669)
                     /locus_tag="VFX87_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX4995461.1"
                     /translation="MLASVFGAGYAPVASGTVGSFVTVVAIWLLPLTPLHIGVALVVV
                     TLIGIWAGSRVERVLGRKDPGVIVIDEVAGMLLSVILLPRTIPVLTTAFLLFRLFDIW
                     KPFPARESQVLTGGVGVMMDDLIAGVYALVLVLGARALFGVPA"
     gene            complement(2701..4026)
                     /gene="rimO"
                     /locus_tag="VFX87_10850"
     CDS             complement(2701..4026)
                     /gene="rimO"
                     /locus_tag="VFX87_10850"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX4995462.1"
                     /translation="MKVHFTTLGCPKNQVDSELMLGMLTEAGHQIVEAAEAADCLVVN
                     TCAFIDRAREESVNTILELAQIKTRRRARALIVTGCLTQRYGGEIRTEIPEINAILGT
                     SELHRIVDLVNQADGRQAWVSEAPPGYLYDAQTPRLLTGRVPYAYVKIAEGCDMGCTF
                     CAIPQFRGRHRSRPLADVVAEVNGLARRGIQEAILVSQDTLAYGRDLPGNGDIGDLLL
                     ALSETPMPWIRPMYLHPAHVTERLIAKWQRARVVPYLDMPVQHGDDGILRAMRRGVTA
                     QRMKDIVARLRDAIPGVTLRTTVLVGFPGETEAAFQNLLGFVEDAGFDRLGVFTYSVE
                     EGTPAADMADQVPAEVMAERAQQVQDLQDRLAWPRQKALFGTRQTVLVDGPSADSAFP
                     FEGRTAGQAPEIDGVVLLRDRTLTPGRFAPGRFAEVAIVEVEGYELVGE"
     gene            4135..4647
                     /locus_tag="VFX87_10855"
     CDS             4135..4647
                     /locus_tag="VFX87_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995463.1"
                     /translation="MPPLQRMSLSLLLALTVLVLAGPAAAHVATPAVPAVVLPATEVP
                     ATMPALTTVATPPAPPWYLPAALALGALAAWHRPRRVVVVMLALLLCVFAFENALHSV
                     HHGFDPQQQEECTLAVASAHLTAVQADDPGPSSVVLPVVGRVEAAPPKPTSTRFLSPD
                     QGRAPPSVIL"
BASE COUNT          698 a         1866 c         1726 g          673 t
ORIGIN      
        1 gcgcgggatg ccgccgatcc ccagcgccac gtcgagctgc atggcgccgg tgggaatgac
       61 ggcgatctcg tcgaacgccc cgttctggcc aaggcgcatg atggcgccct tgccgaactg
      121 gcggtcgatc gcggtaatgg ccagttccag ggcctggcgg cggtcgctct tgacgtcagc
      181 catgctgatt ctcctcgact acgcgtggaa ggggcccgtg accggacccg agaggtagac
      241 gaaaaaggtt cgatagttca acaagttttc ccatgatacg ggcgcgttgc gaccaaagtc
      301 aacgaagcga gtgtgagaga gcgcggaatc cgtgtcaatg tccggcgtcg gatacggcta
      361 cgcgccgagg ctcagcgcgc gccgcaccgt gtaccgagcg cccgacggtg agagatcgct
      421 gcgcatcagc gcgacctcgg tcacatgctg cgtgccgaag gtcgtggtgg cggcctgcgc
      481 gagcgcgctg cgcagtggcg ggcccgcctc gcgccgccag cggcgttcgt cgtggacgcg
      541 cccgatcgtg agatgcggat gccacgcccg ctcctccttg ccgaacccgc gcgcggcgag
      601 cgcgtccgtc acgcgcgcct gcagccggcg cgcctcgagc gcgccctgcg ccaggcccac
      661 ccagaggatg cgcggccgct ccaggccggg gaacgcgccg atgccgtgga aggtgacgtc
      721 gagcggcgcg ctcgcggccg cggcgtcggc cagcgcggcc tcggcttcct ccagacgctc
      781 ctcgctctga tcgcccagga agtgcagcgt gatgtggagg ttacgcggcg gcacccacgc
      841 gaccgccgag cccagcggcc ggaggcgccc gatggtctcc gcgatcgccg cgcgcacgcc
      901 atcgtcgaga acaaccgcga cgaaggcgcg gatcacggcg gtgacagggg cgggtccgcg
      961 acgaaggcgc ggatcacgcc cgcgcgagga gggcgcggcg gagcatatcg agcgcggcct
     1021 gcgaggattg ccacttgacg gcttcccgcc cgcccgcgaa gcggaagcgc cgcacgtcga
     1081 tgcgcgggcc gtcggcgccc ggcgcggccg ccgcgatgaa cacggtgccg accggcttgg
     1141 tgggcgtgcc gccgtcgggc ccggcgatgc cggtcaccgc gatcccgcac gccgagccgg
     1201 agatgcggca gatgcccgcc gccatcgcct ccgccaccgg cgcgctcacg gcgccgtgcg
     1261 cgcgcagcat ctcctccggc accccgagga gctgctcctt ggcctggttg gagtacacga
     1321 ccacgccgcg ctcgacgtag cgggacgagc ccgcgatgtt ggtcagccgg tggccgacga
     1381 gcccgccggt gcaggactcc gccatggaca ccgtgagccg ccgctccagc aggagccggc
     1441 ccaccacgac ttcgagcgtg tcctcgtcct tgccccagca gtcgggcccg agcgcttcgc
     1501 gcacttcacg ctccagcggg acgagcgccg cctccgccgc gctccgggag gacgcgcgcg
     1561 agcggagctg cacccacacg tcgccctcca ccggcacgca cgagacggtg accgcgccct
     1621 cgacgcccag ccagcgcccg agacgctcct cggcgtccgc gggcgagagg ccggtggtgt
     1681 gcagcgtgcg gagcacggtg ctctcgcccg cgccgagccg ctgccgcgcg agcggccgca
     1741 ggtgcgactc cacgagcgag gccagcatcg cggagccggt gggcagcacc gccaccgcgg
     1801 cgcgccggac ctcgagcacc cacgcgggct ctccccccgc gggccagagc agcgcgccct
     1861 gcggcaagag cgccaggcga tcgagccgcc ggggcatggc ccggccgcgc cgggcgaaat
     1921 cctcttccat cgacgccagc agcttgtcgt tcagcaccag ccgcgcgccg ccgagccgcg
     1981 agagcgcgcg ccggaccacc tcgccgctcg agccacccgg ctgcgccagc accaccacca
     2041 gcgcagcggc ctcgacggcg ccgcgcaggg cttcctccac cgccgtctcg tcctcgtcga
     2101 cgacctggcg cgaggccacc ggcacgcctt cggccaggag cgcgcgggcc accatcatcc
     2161 cggccgggtc gtcggccgcg ccgagctgcg ccgcgcccac cgtgacgatc caggccccgc
     2221 tcacggccgg ctcacgcggg gacgccgaag agggcacgcg cgcccagcac gaggaccagc
     2281 gcgtacacgc ccgcgatgag atcgtccatc atcacgccca cgccgccggt gagaacctgg
     2341 ctctcgcggg caggaaacgg cttccagatg tcgaagagcc ggaagagcag gaacgcggtg
     2401 gtgagcacgg ggatggtccg cggcagcaag atcaccgaga gcagcatgcc cgccacctcg
     2461 tcgatcacga tgacgccggg gtccttccgg cccagcacac gctccacgcg cgagccggcc
     2521 cagatgccga tgagggtgac cacgaccagc gcgaccccga tgtgcaacgg ggtgaggggc
     2581 aggagccaga tcgccaccac ggtgacgaag ctgccgaccg tgcccgacgc caccggggca
     2641 tagcccgcgc cgaagacgct ggccagcacg aaggccaggc ggtcgaggag cccgagcggc
     2701 ttattccccg acgagctcgt agccctcgac ctcgacgatg gcgacctccg caaaacggcc
     2761 gggcgcgaag cgccccgggg tgagcgtgcg gtcgcgcagc agcaccacgc cgtcgatctc
     2821 gggcgcctgg ccggcggtcc ggccctcgaa ggggaacgcg gagtccgcgc tcggcccgtc
     2881 caccagcacg gtctggcggg tgccgaagag cgccttctgg cgcggccatg ccagccggtc
     2941 ctggaggtcc tggacctgct gggcgcgctc ggccatcacc tcggccggca cctgatcggc
     3001 catgtcggcg gccggcgtgc cctcttccac cgagtaggtg aagaccccga gccggtcgaa
     3061 gcccgcgtcc tccacgaagc cgagcaggtt ctggaacgcg gcctccgtct cgcccggaaa
     3121 gcccaccagc acggtggtgc ggagggtgac gccgggaatg gcgtcgcgca ggcgggcgac
     3181 gatgtccttc atgcgctggg cggtgacgcc gcggcgcatg gcccgcagga tcccgtcgtc
     3241 gccgtgctgg accggcatgt cgaggtacgg caccacgcgc gcgcgctgcc acttggcgat
     3301 gagccgctcg gtgacgtggg cggggtgcag atacatcggc cggatccatg gcatcggtgt
     3361 ctcggacagg gccagcagca ggtcgccgat gtcgccgttg cccgggagat cgcgcccgta
     3421 cgcgagcgtg tcctgcgaga ccaggatggc ttcctggatc ccgcgccgcg cgagcccgtt
     3481 cacctccgcc acgacgtcgg cgagcgggcg gctccggtgg cgcccgcgga actgcgggat
     3541 cgcgcagaag gtgcacccca tgtcgcaccc ctcggcgatc ttcacgtagg cgtacggcac
     3601 ccggcccgtg agcagccgcg gagtctgcgc gtcgtagagg tagccgggcg gcgcctcgct
     3661 cacccacgcc tgacgcccgt cggcctggtt cacgagatcc acgatgcggt gcagctccga
     3721 ggtcccgagg atcgcgttga tctccggaat ctccgtgcgg atctcgccgc cgtaccgctg
     3781 ggtcaggcag cccgtcacga tgagggcccg cgcccgccgc cgcgtcttta tctgagccag
     3841 ttcaaggatc gtgttgaccg attcctcgcg cgcccggtcg atgaaggcgc aggtattgac
     3901 caccaggcag tcggcggcct ccgcggcctc cacgatctgg tgcccggcct cggtcagcat
     3961 gcccagcatc agctcggagt ccacctgatt cttggggcag ccgagggtcg tgaaatggac
     4021 tttcatgacg ggagcttatg atcccacacc ccctccggtg gcgtcaacgc ggggccatcc
     4081 gtcgatccgg aaccttcctt gacgtggccc ggttcagtgg ttagtttgct tcgtatgccg
     4141 ccgctccaac gcatgtccct gtcgctgctg ctggcgctga ccgtcctggt cctcgccggc
     4201 ccggcggcgg ctcacgtggc gacccccgcg gtccccgccg tggtcctccc ggcaacggaa
     4261 gtccccgcga ccatgccggc cctgaccacg gtcgccacac cgcccgcgcc gccctggtac
     4321 ctgcctgccg cgctggcgct cggggccctc gccgcctggc atcgcccgcg gcgagtcgtg
     4381 gtcgtgatgc tggccctgct cctctgcgtc ttcgcgttcg agaacgcgct gcactcggtg
     4441 caccacggct tcgacccgca gcagcaggaa gagtgcacgc tggccgtggc ctccgcccat
     4501 ctgaccgccg tgcaggcgga cgatcccggg ccgtcttccg tcgtgctccc cgtcgtcggt
     4561 cgggtggagg ccgctccccc gaagccgacc tcgacgcgct tcctcagccc cgaccagggg
     4621 cgcgcccctc cctccgtgat cctgtagctc accgcggtct cccgcccgtg cgctccgtgc
     4681 gccgtggcca cgcgcgggtg ttccggccgt cgcggttcca atcctggccc tttcggaggg
     4741 aagcattcgt gctcaacgag ctgtcgtggt ggcgtgcgat gccgatcggc ctgtctgcct
     4801 gctccgtgtg gcgctctacc cccgatatcg ccttcgcgcc cggcgtggcc cgatgaggct
     4861 cgcggtcctg gtggtcctgg gtatgatcgg gacatgcctg tgggggctcc ccgacgcgca
     4921 cgcgcacgcc atcatcctcg agtccgcccc gcgccacgag gaa
//
LOCUS       DASUFA010000289        25266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_874219, whole genome shotgun sequence.
ACCESSION   DASUFA010000289 DASUFA010000000
VERSION     DASUFA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 25266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25266
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874219"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3294
                     /locus_tag="VFX87_10860"
     CDS             <1..3294
                     /locus_tag="VFX87_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4995464.1"
                     /translation="HDRTFTFLCAPNDTHNCLLRAFVKNNVLVRIEPTYGYGRATDLS
                     GNRASSRWDPRCCQKGLVLGRRLYGDRRIKGAFVRKGFKEWAEKGFPRDPATGRAPAD
                     LMKRGWDGFVRVPYAEAYALHAKALENITRTYSGEPGKQYLLAQGYDPAMVDAMGGAG
                     VRTNKVRGGMAKLGATRIMGTYRFGNMLALLDAAVRGVGADRALGGTAWDSYSWHTDL
                     PPGHPMVHGDQTSDFDLFDVENANLVVVWGMNWIATKMPDSHWLTEARLKGTKVVVLT
                     VEYSATANKGDEVIVIRPGTDPAFALGLAQVIMAEKRYDAAFVKRYTDLPRLIRMDTL
                     GPLLARDVFAGHQESRLANWTHMVKPGEALAPTPQQNAAQMPEALRGEFADFVMWDAA
                     TGKPVAVTRDQVGDHFTARNIDPALEGSFDVTLADGTKIQARPVFDLTRQYLAETLPP
                     ETASKITWAPAPAIVSLAREIAANPGKTLFPVGMGPNQFFNNDLKDRAIFLVAALTGN
                     IGRQGGNVGSYAGNYRTSLFSGVPQYLYEDPFGPQLAADGPVKTHGYLRGESLHYYAA
                     GEVLHREGNKLFTTGPHVPTPTKSIWLSNSNSQIANTKYHYNVVFNTLPRQECVVVNE
                     WWWSGSCEYADIVYGVDSWMELKYPDMTASNTNPFLQVFPRTPLRRNFGTVSDLETYA
                     GVAGALATVTKDQRFADYWKFVTDNRVEVYLQRILDGSAPCKGYNIRDLEALAAQGTP
                     ALMNVRTYPRTFSWEQVHESKPWYTKTGRLEFYRPEPEFVEAGENLIVYREPVDSTFY
                     DPNCIVARPHPALRPKSPEDYGFDRGKRDWSARQGRNVVLTPEAMLATQHPNLAAGYR
                     SVFHTPKYRHGSHTTPIDTDIMSNFFGPFGDMYRRDKRAPHVGEAYVDINPEDARAMG
                     VNDGDYVWVDGDPQDNPFKGWQDKGREAEYHVARLLCRARYYPGTPKGIMRMWHNGYM
                     ATPGSVKGHETRPDGLAKNPETNYQAFFRYGSHQSLTRSWLKPTHQTNSLITRRSYGH
                     ELMRGFQPDVHCVTGAPREAMCRVLKAEDGGIGGKGLWRPVTLGLRPTNESAALKQYL
                     SGGFVIKKA"
     gene            3310..4437
                     /locus_tag="VFX87_10865"
     CDS             3310..4437
                     /locus_tag="VFX87_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX4995465.1"
                     /translation="MAKVYNWQIGREMDYPYEGKRPEKQFAMIFDTNKCIACQTCTVA
                     CKTTWTPGRGQEYMYWNNVETKPYGYYPLGWDVNILDKLGVQEMTGPVYDGKTLFDAA
                     PQGEILLGYLPEDLDYAHPNIGEDDSTGLMPQGVHLTLPHMQWMFYLPRICNHCTYPA
                     CLSACPRQSIYKRPEDGIVLLDQSRCRGYRECVKGCPYKKLYFNAQTRVSEKCIGCYP
                     AVEGGRQTQCTISCIGKIRIQGFMSAPDRVNEDNPLDYLVFVKKIALPLYPQFGLEPN
                     TYYIPPVHVPPVFLRQMFGFGAEQAIATYRTAAKDRKLLGALTLFGATPEISHYFRVD
                     GDTVIGYDAKQAEIVRVPLREPVVVRELYDIKHKAFRTNIT"
     gene            4452..5291
                     /locus_tag="VFX87_10870"
     CDS             4452..5291
                     /locus_tag="VFX87_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX4995466.1"
                     /translation="MRARVAKLALAAVLAWAGAAHAQAPLTVARVSGAGPIADPAAPA
                     WKLVRPVKVAMLPQTVTLPNHPEPAIKELVVRAVHNGGWIAFLIEWKDSSLSDRVVLD
                     NFGDQVAVELPIDIKADPPSPMMGNPNGRVNIMQWRAAFQHDLDQGPPTVRSLYPNAW
                     ADVYPDEVLGAADARPYSGALGIENPISRGRVTPVLDQMAEGWGTMTVKPDQHALGKG
                     AWKNGVWRVVITRPMVSDDLNAPRLLPGNRTVAAFAVWDGGNREVGARKAWASWVPLV
                     IQR"
     gene            5288..6034
                     /locus_tag="VFX87_10875"
     CDS             5288..6034
                     /locus_tag="VFX87_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX4995467.1"
                     /translation="MSADERPIQERLERASLYRLLGGALARPQAARLAELAAAAEALA
                     PAVEPAVAEALARFAALARETDAAVVGDEYVFLFDRGAKVPPYEGAWEDAPQLAGKAA
                     LLADVAGFYAAFGLVPGEAQPDVEDHIAAECEFMSALALKEAYALAESDDEGVAITRA
                     AQSRFVGDHLGRWSGTFAEALRDASPLPYYGALADLLGAWVRAEIERLGAAPSVVLGR
                     SGYDTIQEADTFSCPMGEPEASPEDQDEEP"
     gene            6021..6662
                     /locus_tag="VFX87_10880"
     CDS             6021..6662
                     /locus_tag="VFX87_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4995468.1"
                     /translation="MKSPETNGNGLDEALSHTADGAFAIGADGKVLLWNRAAEKMLGY
                     TAREALGRACCDLFVGYDDSGNRLCYQGCHVMSLVRIGETVQSFDMRTRTKAGRPIWI
                     NLSTLTVPAGHVGGALTVHLFRDVTATKELLGLVRERLTAPAAAEANGDTLTRREQEI
                     LRTIASGMSTKAAADKLHVSPATVRNHVQNILGKLGAHSRLEAVAMANRQRLL"
     gene            6708..7160
                     /locus_tag="VFX87_10885"
     CDS             6708..7160
                     /locus_tag="VFX87_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX4995469.1"
                     /translation="MRILRWLGIALIVALVVVQLVPYGRRHTNPPARVEPRWDVPATR
                     TLAVRACYDCHSNETVWPWYSHVAPVSWLAQRDVDEGRRKLNFSEWDRPQKEARESAK
                     TVRKGEMPPWFYVLINPDARVTAAETQALIAGLGTTLGTKGGKGRDDD"
     gene            7312..7656
                     /locus_tag="VFX87_10890"
     CDS             7312..7656
                     /locus_tag="VFX87_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995470.1"
                     /translation="MGRRGFVIGLAAAAVSLLAGKGIAQGPPAGKGRGPRWDQEAEQS
                     FRMGRGTGRKLMTEDEWKEHRAKMRGMTPEERSRYREETHKQMMERAKEKGITVPETP
                     GPRGPKGPGPRP"
     gene            7667..8920
                     /locus_tag="VFX87_10895"
     CDS             7667..8920
                     /locus_tag="VFX87_10895"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX4995471.1"
                     /translation="MAPEPPVYRPFALLGLAVTLAGGIPLGVRLLAWLYLGATAVRAE
                     WILLHAHVQIFGFFGTLILGVAQHLLPRFTGRPVSPSPFMLWLLGLQAAAIVLRIAGT
                     AGPWPVPVLVASLLQAGVFLLFAIWVWRMLDPPPLGFLRWHLTASTLWLAGGCALEAW
                     LRVDALASGLALPAGAGLRVVHLMGLFGGVLGWVLGVLLRAGPMFLARWRAPARAARV
                     LPWILALGVGVAAWGEAGDWSAAVGTTLARLGEVIVLAGAAAVMVLGGALTRVRDTLP
                     LVARSREESRIFRVAMLSGAAAAVGSAAAVPAAWAGLDVRLLADAVRHLITVGVLTSV
                     VVAMVFRLIPVLEGRALPWPRLRHVALWCLAAGVVLRSAQVLVGAGWAAPAPWVALSG
                     VLVWIAVACVGANLARAIGMSAGGA"
     gene            8917..9081
                     /locus_tag="VFX87_10900"
     CDS             8917..9081
                     /locus_tag="VFX87_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995472.1"
                     /translation="MTNGQAGSGRCLTAEAWVVVARTTRVAVLGARVQAEGDRLVALA
                     TGSFYIRPVR"
     gene            9179..9580
                     /locus_tag="VFX87_10905"
     CDS             9179..9580
                     /locus_tag="VFX87_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4995473.1"
                     /translation="MRSVVCRAVVVVAVGVGLGLAGAAWAQTPWTAPESEKGKKSPVA
                     ASHKVAEQGKKVAEVNCVSCHGKSGKGDGAAAIALNPKPADWTSKKIQEESDGEVFWK
                     ITTGRGAMPAWRHLPESDRWALVQYIRTLKK"
     gene            9687..10307
                     /locus_tag="VFX87_10910"
     CDS             9687..10307
                     /locus_tag="VFX87_10910"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4995474.1"
                     /translation="MAIGERYEGILNAACSVIARRGFHQASIREIARAAGLSLAGLYH
                     YVGEKDELLFLVLGRSLDRLLESLGRNLAEAPTPEGRLLALIQTHLDFGFHHAQALRI
                     INRDHELLAEPRRAEIAAKRQAYLQCGLGVLRELDPHGRSDAELFSATNLLLGMLNGI
                     ATRPFLRSADDARALAAEVGALFLHGFLERAGRVEEPSSFALRRPA"
     gene            10304..11458
                     /locus_tag="VFX87_10915"
     CDS             10304..11458
                     /locus_tag="VFX87_10915"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX4995475.1"
                     /translation="MTPSLILERCRDLIAQPLGAVAERWKAEHPGGRAVAAYPVWAPA
                     ELVHAAGMLPLSLLGGGTSVELTHADARFQSFVCSIAKSSLELGFQGLVKGVDGFVFS
                     NICDVARNLSSIYKRNFPDTFVEYLHLPQNSTSPAVIGYAAAELRRLGACFDSAFGLA
                     ATPAALAKSIEIYNALRARLRALYGLRIAEPQKLSTAELYVVLRATTLAPPEEAIAWL
                     DVLLDALPRRESRPRDRLRVVVEGAFCEQPPLGLLEVLEEAGCYVVEDDLLLGWRWFT
                     SDVASDGDPFERLGAAYVNQAVPSSTRHEGRQHRSEGLIEKVRRARADAVVFMPAKFC
                     EPALFDYVLMKQGLERAGIPHLLVEFEEKMWTFERTRNEIETFVESMLFE"
     gene            11625..12935
                     /locus_tag="VFX87_10920"
     CDS             11625..12935
                     /locus_tag="VFX87_10920"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX4995476.1"
                     /translation="MTGVPVTERQYQGQAHEHSRRLMTAWMEELARAEAEGQPTGALM
                     ISGNCVELLRACHILPMFPEVTALQNAIRKKSLPLILKAEQAGYSSDNCAYVKADIGL
                     FLDGGMGPGKPIPFPTITVCNYVGCNVYVKWFEHLADISGSKLFMLDVPFARTAEPSP
                     ADVQYVVAQLRELIALCESVSGKKFDIDELREILAHAARAEGGYARAKELCKRHPAPF
                     DAYFDAINMMGPINVLRGTRDAAEFFDTAVAEFEGLVAQGLGPLGEERFRTVVEGPPP
                     YPFYKNFRNLFAKWGAVAVASTYSTVGGIWEFGFRHDPRRPLESIAEQMLRENLTNRS
                     IVARYGQIKRYVEEWEADALVIHSIKSCRLFSAGQGDMRDYFTQTLGVPTLMVESDLE
                     DPRYYAEAQLKNRIDAFFESLEHKKLTAGRAAAAGEASEYEKRA"
     gene            12919..15771
                     /locus_tag="VFX87_10925"
     CDS             12919..15771
                     /locus_tag="VFX87_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4995477.1"
                     /translation="MKSARSATGEARLQKIPVYCYQCVAGPDLLRVVVKDGVAVGVEP
                     NPEMADVHPACGTVCVRAYALIQKLYNPARIQTPMRRTNPRKGRDQDPGWEPITWEEA
                     LGTLAARLNRLRASGLVDGAGYPRLAVTMGSGGIAPAYLGTLAAFLAAWGPVDQGIGS
                     GQGVKCYHSEHLYGEFWHRAFTVAADVPRCDFVLSFGYNGDASGGVTGVYRHAEARAR
                     GLTWVQLEPHLSVTGAGATEWVPIRPKTDAAVLFALLHAILLEHDWRAVCDVPFLERM
                     TSSPYLVGPGGYYLRDPKTLKPLIWDQGKDRAVPFDDPDCVRPAMEGEYIAAGVEIGA
                     DGARRSVSERVRPAFARLIEHVKPYTPEWAAVIADVPAETIRRLAHEYVGHARVGATI
                     AIEGVKYPHRPVAVLLGKTVTNGWGGYECCWARTVLAALVGALEVPGGILGTTVRLNR
                     PAQNRQDSVRPGPDGFMEQPLNPTERGKWQGAPHIRNAYRTLVPLADNSPWSAALGPA
                     HLPWLFMENPPEAWPAPTVPDVWIIYRTNPAISNWDTGRIERQLERFPFVAAFAYTQD
                     ETNWYADLLLPEATDLESLQLYRIGGTKYIEQYWQHTGWALRQPVVEPPVATRDLTDI
                     ATELAARTGLLEEYLAALNRGSGTTVPLKGDDYDYSFREPVRPMADEIWDRVCRAATV
                     SLSRGAVERDLEWFKRHGAFFVPFSSKQYFLHGTMVAQGLRYELPYQERIKRVGEELG
                     ARLHERGIKWWDVQLDEYQPLPGWKDFPGIWQEPARLKGRAPGEFPFWLLTSRSMQYS
                     WGSNVSLPVLADVARHVKGHFGVMLNRGAAQRLGIADGDLVEIESPTGRTQGRAVLRE
                     GVRPDVAVVLQQFGHWATPFARDLGMPNLNQVASMDLALTDATGSGADLVPVAIRPAP
                     GLRETAAPTEAGFRMPVPLRHPRMDLEICEMYMRGL"
     gene            15771..16409
                     /locus_tag="VFX87_10930"
     CDS             15771..16409
                     /locus_tag="VFX87_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX4995478.1"
                     /translation="MTRWGMAIDVKRCVGCQTCTIACKQEHGLPADTAWRFVADCEVG
                     EYPDVRRLFLPMQCMHCAEPPCVPVCPTGASRQRDDGIVWVHYNACVGCGYCAMACPY
                     QARHLIHSADGYFESPTPSEAAQTRPERHGVMTKCTFCKDRVDDGLAHGLRPGVDPDA
                     TPMCALACIAGAIVFGDLDDATSRVCHLAADPRAQVLMPECGTEPRVYYLAD"
     gene            16413..17291
                     /locus_tag="VFX87_10935"
     CDS             16413..17291
                     /locus_tag="VFX87_10935"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX4995479.1"
                     /translation="MATAMTAQRMEMMPARRQELWGWPAVVNFALGGLGAGWYVVAVL
                     EAGFERSPGVTAASWAASALVLAGFAAVAAEAGRPLRGPRVLTRLRTSWMSRELLAGI
                     AFVLLVAADLAFPLRLYRAPAVVAAVLLALAQGFIVRRARGVTAWDVPLMPLLFLLSA
                     LVSGAGAYLLAEVAAGRPPLPALVGAVLALVVVAFVAWARYLAWTSEDTYRRAVAPLR
                     EGRAALLIDGAGYGLPLLLGLLALAAPAVAGPLTGLAGTLLIAGQAYAKARLIRAAGR
                     LRPITLGITLSRRRSA"
     gene            17288..18112
                     /locus_tag="VFX87_10940"
     CDS             17288..18112
                     /locus_tag="VFX87_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX4995480.1"
                     /translation="MIYGTGVDVGSTQTKAVIVDESRRIVARALIATGANVSRAGENA
                     FVRACESAGLPREAVGYVVGTGYGRYKVTFGDAQITEITCHARGAQSLFPATRTVIDM
                     GGQDTKAIKVGPDGSVVDFSMNDKCAAGTGRFLSAAADVTGVPLDEIGPLSLQAKTPV
                     RLTSVCTVFVESDIMSYLAQRKTVEDILGGVHKAIATRTMSLVRRVGIEDEITFTGGV
                     SRNTGMVRALEEVLGKPINVSGEGHYMGALGAALFALERAQAGARGEAVATGAGRS"
     gene            18115..18915
                     /locus_tag="VFX87_10945"
     CDS             18115..18915
                     /locus_tag="VFX87_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX4995481.1"
                     /translation="MLVAGIDIGSGTTKCVLVDETGTVRGRGQTKTRADFEKVAREAV
                     ATALDAAGFHGDAIGYTATTGLGRYAVPFRDIQITDLTCGARGAATACPEARFVLDIG
                     AQCSRAIRLRDGGKVKEFHMNEKCAAGSGGFLERAAKYLEVTLADIGPLSLHAARPQA
                     ISSVCAVLAETEIINHVSEGVAVENILRGIHNSLADRALSLLKRVGLDGPVAFIGGVA
                     RQEGMLVALRDKLGVPVHVPDGPQFAAALGAALLGRQRFLKTGRPAAA"
     gene            18927..19379
                     /locus_tag="VFX87_10950"
     CDS             18927..19379
                     /locus_tag="VFX87_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4995482.1"
                     /translation="MSRSAINVRPAELSDQAAIVQIDEKLSGLTRKDYWQQRLEIAAL
                     RPPWMSLIAETDGRPVGFLFGWVGESEFGIARRTGWVDLIGVAPPYRGRGVAQALLRR
                     FVDSGRELRAIERVATLIDLGQEDVREFFTREGFHHGPMIHMEREVLP"
     gene            19376..19918
                     /locus_tag="VFX87_10955"
     CDS             19376..19918
                     /locus_tag="VFX87_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX4995483.1"
                     /translation="MRTVASPTQTLREEHRVILRALGLVEVAAGRLDAGRALPAGWWE
                     ELLEWLRAFADRNHHAKEEAYLFPALAEAGVPAAGGPVGVMLDEHTEGRGLIRLMAES
                     PVERRAAAARRYVHLLRDHIDKENGVLLPLADAVLEPGAQLGVARAFEQIEAEQGPAA
                     SLDHAETLVDRLAAALGDRA"
     gene            complement(19956..20387)
                     /locus_tag="VFX87_10960"
     CDS             complement(19956..20387)
                     /locus_tag="VFX87_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX4995484.1"
                     /translation="MSLLVRWLHVLSAITWIGGMLFIALVMVPIARRLEDPILRTRLI
                     RDTGRRFRAVGWAAIGVLIVTGLLNLWMHPVLLSSTRFHWKLGLVVLALILSAFHDFV
                     LGPRAGAPGADPSARVRASWVARINVLVALAIVAFGLSLFG"
     gene            20361..21170
                     /locus_tag="VFX87_10965"
     CDS             20361..21170
                     /locus_tag="VFX87_10965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4995485.1"
                     /translation="MKPADEERHVGQDSTYSRSAIWTEQTAAWYRRAAERGDYAARVL
                     EAIGSRLDLCGTALDVGAGCGALAIPLARRLRSVTALEPSPAMARELRRWAVEAGLDN
                     IAVVETAWGEGRVGAHDLVLCAHVGNLFRAESAFLREVGRVARRLVVLVRDTTSASGG
                     DKFFYSELYPILLGRPYATRGDADDTVAALRSLGIRPAVTEIEYRSDQPFADLAEACE
                     FWMAYLGLSDAGSRTYLAGFLGERLVRCDGGWIAPFRKSAAVITWGGSSWL"
     gene            21161..21760
                     /locus_tag="VFX87_10970"
     CDS             21161..21760
                     /locus_tag="VFX87_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX4995486.1"
                     /translation="MALAPSIIREEHRSLFAVIHSFRTLVRDARDRGHAPDYELFTLI
                     LDYIEGFSDRFHHPKEDEHLFAALRRRCAEANEALDVLERDHARGDELVRDLRYLLSR
                     CRVGGAAAVEAFAAAVEEYVEFYWRHMRLEEDAILPLAERTLTDVDWQPIDAAFRANE
                     DPLFGARPREEFRKLFQLIVNMAPAPVGVGPSPGTRPAP"
     gene            22011..22688
                     /locus_tag="VFX87_10975"
     CDS             22011..22688
                     /locus_tag="VFX87_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4995487.1"
                     /translation="MKAQDSMTHDVITVTPTTPIHEVAALMANHGVSGLPVLDEKGSV
                     VGIVTEADLLVRQKPRARMSWWSLFLDGERMAREFQRAVGTTAGEVMTREVISIGPDL
                     PIEAAAAILHGHGIRRVPVVADGVLVGILSRGDLVKALAGAPPRTAAPVSDARLVETM
                     KAGMAAEVWAPRGIVVEAVAGVLTLRGLVGTQAECAALETMARSIPGVAGVDNQLVVN
                     PVLPSGV"
     gene            22702..24489
                     /locus_tag="VFX87_10980"
     CDS             22702..24489
                     /locus_tag="VFX87_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4995488.1"
                     /translation="MLNGGEIIVEYLAREKVPYVFGLCGHGNVGLLDALYDRTDAIRT
                     ISTRHEQTAGHMADAYFRVAHRPVATLTSCGPGSANLPIALACAFMDSSALLAITGNI
                     PTSQFNRAAFQETYRHFQADFPAVIRPYVKRSWQPTRTDMLPTALRQAFATMLGGRPG
                     PVNLDVPFNVFKEEAEVDVPEPGAWRTGISSRSGGEPDAIQRVVELLRQARRPLIYVG
                     HGVTLSEAGDELTAFARRLRIPVIFSPNGFGALDMADPLAVGFVGRNGVYQANEAART
                     CDVLLALGVRFDDRSSSSWIPGYSFNIPPTRLIHVDIDGEEIGRNYPVHLGIVGDART
                     VLRQLLAATEGSGAEAPAHDAWRADIATWRAQWEEFVRPHETSEAEPIHPQRVVQALR
                     AVMPDDGIVLPDVGVHHNWMVQFWRARRPQTLLNTWGFGAMGFGVCGVLGAKLAAPDR
                     PCVTVCGDGGFMMRPDVLCTAVEYDIPAVWVVWNNFAYGGIRDLQLGMFAKREIATSF
                     VREKDGRLVNPDFVALARACGADGIRVERPADLAGALEHAIKADAPFVLDVHVDRDIR
                     PPGVGTWELPPLPYGEPAFGKRRLPREGE"
     gene            24502..24915
                     /locus_tag="VFX87_10985"
     CDS             24502..24915
                     /locus_tag="VFX87_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4995489.1"
                     /translation="MAGRAACKAKIMRMEEAFWELPPGHFQAFSKLLVHPTNAETRFF
                     DFRISSYQPRGYAEVHVHEVAENLYYILQGKGIVELDGVRHVVEPHMVVHIPPGVRHG
                     IFNTGLEDLVFLVVASPPADMPTVSQTGGRDVEAR"
     gene            24919..>25266
                     /locus_tag="VFX87_10990"
     CDS             24919..>25266
                     /locus_tag="VFX87_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995490.1"
                     /translation="MAGYPRLFSAGRIGSLALRNRILMAPMEKNLALPTGAVSGRYID
                     YCEARAAGGAALILLESMYVHPAGKNHRYQLGIHDDDLVPGYRRLIEACHRHGALVGA
                     QLQFAGRETSSAVT"
BASE COUNT         3760 a         8839 c         9011 g         3656 t
ORIGIN      
        1 cacgaccgca cgttcacgtt cctgtgcgcg cccaacgaca cgcacaactg cctgctgcgg
       61 gccttcgtca agaacaacgt cctggtgcgg atcgagccca cctacggcta tggccgagcc
      121 acggatctct ccggcaaccg ggcctcgtcg cgctgggatc cgcgctgctg ccagaagggc
      181 ctggtgctgg gccggcggct ctacggggac cgacgcatca agggtgcctt cgtgcggaag
      241 ggcttcaagg agtgggccga gaagggcttc ccgcgcgacc ccgccaccgg gcgggcgccg
      301 gcggacctga tgaagcgcgg ctgggacggc ttcgtccgcg tgccgtacgc ggaggcttac
      361 gcgctgcacg cgaaggcgct ggagaacatc acccggacct acagcggcga gccgggcaag
      421 cagtacctgc tggcgcaggg ctacgacccg gcgatggtgg atgcgatggg cggcgccggg
      481 gtccgcacga acaaggtgcg gggcggcatg gccaagctgg gcgccacccg catcatgggc
      541 acctaccgct tcggcaacat gctcgcgctg ctcgatgcgg ccgtgcgcgg cgtcggggcc
      601 gaccgggcgc tgggcggcac cgcgtgggac tcctactcct ggcacacgga cctgccgccg
      661 ggccacccga tggtgcacgg cgaccagacc tcggacttcg acctcttcga cgtcgagaac
      721 gccaacctgg tggtggtctg ggggatgaac tggatcgcca ccaagatgcc cgacagccac
      781 tggctcaccg aggccagact gaaggggacc aaggtcgtcg tgctcacggt ggagtacagc
      841 gccaccgcca acaagggcga cgaggtcatc gtgatccggc ccgggaccga ccccgcgttc
      901 gccctggggc tcgcccaggt catcatggcc gagaagcgct acgacgccgc cttcgtcaag
      961 cggtacacgg acctcccgcg cttgatccgg atggatacgc tcggaccgct cctggcccgg
     1021 gacgtcttcg ccggccacca ggaatcgcgc ctcgccaact ggacgcacat ggtgaagccg
     1081 ggcgaggcgc tggccccgac gccccagcag aacgcggcgc agatgcccga ggcgctccgc
     1141 ggcgagttcg ccgacttcgt gatgtgggac gccgccaccg gcaagccggt ggcggtcacg
     1201 cgcgaccagg tgggcgatca cttcaccgcg cggaacatcg atccggcgct ggaaggctcc
     1261 ttcgacgtga cgctggcgga cggcacgaag atccaggccc ggccggtgtt cgatctcacc
     1321 cgccagtatc ttgccgagac tctgcccccc gagaccgcgt cgaagatcac ctgggcgccg
     1381 gctccggcca tcgtctcgct cgcgcgcgag atcgcggcca atcccggcaa gacgctcttt
     1441 ccggtgggca tgggccccaa ccagttcttc aacaacgatc tcaaggatcg cgccatcttc
     1501 ctggtcgcgg cgctcaccgg caacatcggc cggcagggcg gcaacgtggg ctcctacgcc
     1561 ggcaactacc gcacgtcgct cttctccggc gtgccccagt acctttacga ggacccgttc
     1621 ggcccgcagc tggccgccga cggcccggtg aagacccacg gctatctccg gggcgagtcg
     1681 ctccattact acgcggcggg cgaagtcctg caccgggaag ggaacaagct gttcaccacc
     1741 ggtccgcacg tgcccacgcc gaccaagagc atctggctca gcaactccaa ctcccagatc
     1801 gccaacacca agtaccacta caacgtggtg ttcaacacgc tgccgcggca ggagtgcgtc
     1861 gtggtcaacg agtggtggtg gtcgggctcg tgcgagtacg ccgacatcgt ctacggggtg
     1921 gactcgtgga tggagctcaa gtatccggac atgaccgctt ccaacaccaa cccgttcctg
     1981 caggtcttcc cgcggacgcc gctgcgccgc aacttcggca cggtcagcga tctcgagacc
     2041 tacgcggggg tcgcgggagc cctggccacc gtcacgaagg accagcggtt cgccgactac
     2101 tggaagttcg tcaccgacaa ccgggtggag gtctacctcc agcgcatcct cgacggcagc
     2161 gcgccctgca agggctacaa catccgcgac ctcgaggccc tcgccgcgca ggggaccccg
     2221 gccctcatga acgtgcggac ctatccgcgc acgttctcct gggagcaggt ccacgagtcc
     2281 aagccctggt acaccaagac cgggcggctc gagttctacc ggcccgagcc tgagttcgtc
     2341 gaggccggcg agaacctcat cgtctaccgg gagccggtgg actcgacctt ctacgatccc
     2401 aactgcatcg tggcccggcc gcatcccgcg ctccgcccaa agagccccga ggactacggc
     2461 ttcgatcgcg gcaagcggga ctggtcggcc cgccaggggc gcaacgtggt gctgacgcct
     2521 gaagcgatgc tcgccacgca gcacccgaac ctcgcggcgg gctaccggag cgtgttccac
     2581 acacccaagt accgtcacgg ctcgcacacg acgcccatcg acaccgacat catgtcgaac
     2641 ttcttcgggc cgttcgggga catgtatcgc cgcgacaagc gcgcgccgca cgtgggcgag
     2701 gcctacgtgg acatcaaccc cgaggacgcc cgggccatgg gcgtcaacga cggcgactac
     2761 gtctgggtgg acggggatcc ccaggacaac cccttcaagg gctggcagga caagggacgc
     2821 gaagccgagt accacgtggc gcgcctgctc tgccgcgcgc gctactaccc cggcacgccc
     2881 aaggggatca tgcgcatgtg gcacaacggc tacatggcga cgccgggctc ggtgaagggg
     2941 cacgagacgc gccccgacgg gctcgccaag aatccggaga ccaactacca ggccttcttc
     3001 cggtacggct cccatcagag cctgacccgc tcgtggctca agcccacgca ccagaccaac
     3061 tcgctgatca cgcggcggag ctacggccac gagctgatgc ggggcttcca gcccgacgtg
     3121 cactgcgtga cgggggcccc gcgcgaggcc atgtgccgcg tgctcaaggc cgaggacggc
     3181 ggcatcggcg gcaagggtct gtggcgcccg gtgaccctcg ggctccggcc caccaacgaa
     3241 agcgccgcgc tgaagcagta cctgtccggc gggttcgtga tcaagaaggc gtgaggggga
     3301 ggctccatca tggccaaggt ctacaactgg cagatcggtc gcgagatgga ctacccctac
     3361 gaggggaagc ggcccgagaa gcagttcgcg atgatcttcg acaccaacaa gtgcatcgcc
     3421 tgccagacgt gcaccgtcgc gtgcaagacc acgtggacgc cgggccgcgg gcaggaatac
     3481 atgtactgga acaacgtcga gaccaagccc tacggatact acccgctcgg ctgggacgtc
     3541 aacatcctcg acaagctggg cgtccaggag atgaccggtc cggtctacga cggcaagacc
     3601 ctcttcgacg ccgcccccca gggcgagatc ctcctgggct acctgccgga ggatctcgac
     3661 tacgctcatc ccaacatcgg cgaggacgac tcgacgggcc tgatgcccca gggagtgcac
     3721 ctcacgctcc ctcacatgca gtggatgttt tacctccccc gtatctgcaa ccactgcacc
     3781 tatcccgcgt gcctgtcggc ctgcccgcgg cagtccatct acaagcggcc cgaggacggc
     3841 atcgtgctgc tcgaccagag ccgctgccgc ggctaccggg agtgcgtcaa gggctgcccc
     3901 tacaagaagc tctacttcaa cgcgcagacg cgggtctcgg agaagtgcat cggctgctac
     3961 cccgcggtcg agggcgggcg gcagacccag tgcacgatct cctgcatcgg caagatccgc
     4021 atccagggct tcatgagcgc gcccgaccgt gtcaacgagg acaatccgct cgactatctc
     4081 gtcttcgtga agaagatcgc gctgccgctc tatccccagt tcggtctcga gccgaacacc
     4141 tactacatcc cgccggtgca cgtcccgccc gtcttcctcc gccagatgtt cggcttcggg
     4201 gccgagcagg cgatcgcgac gtaccggacg gcggccaagg accgcaagct cctcggcgcg
     4261 ctcaccctgt tcggggccac gcccgagatc tcgcactact tccgggtgga cggcgacacg
     4321 gtcatcgggt acgacgccaa gcaggccgag atcgtccggg tgccgctccg ggagccggtg
     4381 gtggtccgcg agctctacga catcaagcac aaggccttcc ggaccaacat cacctgagag
     4441 gagatctggc catgcgtgct cgagtagcga agctggctct ggccgcggtt ctggcctggg
     4501 cgggcgccgc ccacgcccag gcgccgctca ccgtcgcgcg tgtgagcggg gccgggccca
     4561 tcgccgaccc ggcggcgccc gcgtggaagc tggtgcgacc ggtgaaggtg gccatgctgc
     4621 cccagaccgt cacgctgccc aatcatccgg agcccgccat caaggagctg gtcgtccgcg
     4681 cggtgcacaa cgggggctgg atcgcgttcc tgatcgagtg gaaggattcg agcctctccg
     4741 accgggtcgt cctcgacaac ttcggggacc aggtggccgt cgagctgccc atcgacatca
     4801 aggcggatcc gccgtcgccc atgatgggca atccgaacgg ccgcgtgaac atcatgcagt
     4861 ggcgggccgc cttccagcac gacctggacc agggcccgcc gaccgtgcgc tccctgtacc
     4921 cgaacgcgtg ggcggacgtc tatcccgacg aggtgctggg tgccgccgac gcgcgaccct
     4981 acagcggcgc gctggggatc gagaacccga tctcgcgcgg gcgggtcacg ccggtcctgg
     5041 accagatggc ggaggggtgg ggcaccatga cggtcaagcc ggaccagcat gccctcggca
     5101 agggcgcctg gaagaacggg gtgtggcggg tggtgatcac gcgacccatg gtctccgacg
     5161 acctcaacgc gccgcgcctc ctgcccggga accggaccgt ggccgcattc gcggtgtggg
     5221 acggcggcaa tcgggaggtc ggcgcgcgca aggcgtgggc ctcctgggtg ccgctcgtga
     5281 tccagcgatg agcgccgacg agcgcccgat ccaggagcgg ctcgagcggg cgagcctgta
     5341 ccgcctgctg ggcggcgccc tggcccggcc ccaggccgcg cgcctcgcgg agctggcggc
     5401 ggcggccgaa gccctcgcgc ccgcggtgga gccagcggtg gcggaggcgc tcgcgcgctt
     5461 cgccgcgctc gcccgcgaga cggacgccgc ggtcgtcggt gacgagtacg tgttcctgtt
     5521 cgatcgcggc gccaaggtcc ctccctatga aggcgcctgg gaagatgcgc cgcaactcgc
     5581 gggcaaggcg gccctgctgg ccgacgtcgc cggcttctac gcggcgttcg ggctcgtgcc
     5641 cggggaggcc cagcccgacg tggaggacca catcgcggcc gagtgcgagt tcatgagcgc
     5701 gctcgcgctg aaggaagcct acgccctcgc ggagagcgac gacgaaggcg ttgcgatcac
     5761 ccgcgcggct cagtcgcgct tcgtcggcga tcacctcggc cgatggtcgg gaacgttcgc
     5821 ggaggcgctc agggacgcgt cacccctgcc gtactacggg gccctcgccg acctcctggg
     5881 ggcctgggta agagccgaga tcgagcgcct cggcgccgcc ccgagcgtcg tcctcggccg
     5941 ctcggggtac gatacgatcc aggaggccga caccttctcc tgcccgatgg gcgagccgga
     6001 ggcatcaccg gaggaccaag atgaagagcc ctgaaacgaa cggtaatggt ctcgacgagg
     6061 cgctgtccca caccgcggac ggggcgttcg ccatcggcgc ggacggcaag gtcctcctgt
     6121 ggaaccgcgc cgccgaaaag atgctgggct acacggcccg cgaggcgctc ggccgggcgt
     6181 gctgcgacct cttcgtcggc tacgacgaca gcggcaaccg cctctgctac cagggctgcc
     6241 acgtcatgag cctcgtgagg ataggcgaga cggtccagag cttcgacatg cgaacccgga
     6301 cgaaggcggg ccgtccgatc tggatcaacc tcagcacgct gacggtgccg gccgggcacg
     6361 tcggcggcgc gctcaccgtc cacctcttcc gtgatgtcac cgcgaccaag gagctgctgg
     6421 ggctggtgcg ggagcggctg accgcgccgg ccgccgccga ggccaacggc gacacgctca
     6481 cgcgacggga gcaggagatc ctgcggacga tcgcgagcgg gatgagcacc aaggccgcgg
     6541 ccgacaagct ccacgtcagc cccgccaccg tgcgcaacca cgtccagaac atcctcggca
     6601 agctcggcgc ccacagtcgc ctcgaggccg tcgcgatggc gaaccgccag cggctgctgt
     6661 gaggccccgc cggctgctat gaggccccgg cggctgctat gaggccaatg aggattctgc
     6721 gctggctcgg gattgccctg atcgtggcgc tcgtcgtcgt gcagctggtg ccctacggcc
     6781 gccgtcacac caatccgccc gctcgcgtcg agccgcgctg ggatgtgccg gccacgcgga
     6841 ccctcgcggt gcgcgcctgc tacgactgcc acagcaacga gacggtgtgg ccgtggtact
     6901 cccacgtggc cccggtgtcg tggctcgccc agcgcgacgt ggacgagggg cgtcgaaagc
     6961 tcaacttctc ggagtgggac cggccccaga aggaggcgcg cgagtcggcc aagaccgtgc
     7021 gcaagggcga gatgccgccc tggttctacg tcctgatcaa ccccgacgct cgggtcaccg
     7081 cggcagagac ccaggcgctg atcgcggggc tcgggaccac gctcgggacg aagggtggga
     7141 aggggcgtga cgatgactga gcaggcccca ccgcggtccg atcggccggc cgccggctcc
     7201 gtctttcacg cccgggcccg agcacgcgcc ggaagccggg ccggagagtg ccgtcctgcg
     7261 gacgctcccc gcaggaacct agcaggtcga gagaggacca cgacaatgtt catgggacgc
     7321 agagggttcg tgatcggtct ggccgccgcg gcggtgtcgc tgctggcggg gaagggcatc
     7381 gcgcaggggc cgccggcggg gaagggccgg gggccacgat gggaccagga agcggagcag
     7441 agcttccgca tggggcgcgg cacgggccgc aagctcatga cggaggacga gtggaaggag
     7501 caccgggcca agatgcgcgg catgacgccc gaggagcggt cgcgctaccg ggaggagacg
     7561 cacaagcaga tgatggagcg cgccaaggag aagggcatca ccgttcccga gacgccgggc
     7621 ccgcgcgggc ccaaggggcc cgggccgcgc ccgtaacccg ggggcgatgg ctccggaacc
     7681 gccggtctac cgaccgttcg ccctgctcgg cctggccgtg acgctggcgg ggggcatccc
     7741 gctcggcgtc cggctcctgg cctggctcta cctgggcgcc accgcggtcc gggcggagtg
     7801 gatcctgctc cacgcccacg tgcagatctt cggcttcttc ggcaccctga tcctgggcgt
     7861 ggcccagcac ctcctgcctc gtttcaccgg ccgtccggtg tcgccgagcc cgttcatgct
     7921 ctggctcctc ggcctgcagg cggccgccat cgtgctgcgg atcgccggca ccgccggccc
     7981 ctggcccgtc ccggtcctgg tggcgtccct gctccaggcg ggggtctttc tgctcttcgc
     8041 catctgggtg tggcgcatgc tcgacccgcc cccgctcggc ttcctgagat ggcacctcac
     8101 cgcgagcacc ctctggctgg cgggcggctg cgcgctcgag gcctggctgc gcgtggacgc
     8161 gctcgcctcg ggactggccc tgcccgccgg ggcggggttg cgcgtcgtcc atctgatggg
     8221 gctcttcggt ggcgtcctcg gctgggtcct gggtgtgctc ctgcgggccg ggccgatgtt
     8281 cctcgcccgc tggcgggcgc ccgcgcgggc ggcacgcgtc ctgccgtgga tcctggcgct
     8341 cggcgtcggc gtcgccgcct ggggcgaggc cggagactgg agcgccgcgg ttgggaccac
     8401 gctggcccgc ctcggcgagg tcatcgtgct ggcgggcgcc gcggcggtca tggtgctggg
     8461 cggtgccctc acacgggtcc gtgacacgct gccgctcgtg gcgcggagcc gggaggagtc
     8521 gcgaatcttc cgcgtggcga tgctctccgg tgccgcggcc gcggtcggct cggccgcggc
     8581 ggtgccggcc gcgtgggcgg ggctcgacgt gcgcctgctc gccgacgcgg tccgccacct
     8641 gatcacggtg ggtgtcctga cctccgtggt ggtcgcgatg gtgttccgcc tcatcccggt
     8701 gctcgagggc cgcgcgctgc cgtggccacg cctgcgacac gtggcgctct ggtgcctggc
     8761 cgccggcgtc gtcctgcgga gcgcgcaggt gctggtcggc gccggatggg cggcccccgc
     8821 cccctgggtc gcgctgtccg gcgtcctggt ctggatcgcg gtcgcgtgcg tcggcgccaa
     8881 cctggcccgt gccatcggca tgagcgcggg gggcgcgtga cgaacggcca ggcgggctcc
     8941 ggacggtgcc tcaccgccga ggcgtgggtg gtggtggccc ggaccacccg ggtcgcggtg
     9001 ctgggagccc gcgtgcaggc cgagggcgac cgcctggtcg cgctggccac cggatcgttc
     9061 tacatccgcc ccgtccgttg accggccgtc ccatgaccgg ggtcataccc gtccggcccg
     9121 cgatggccga tcctgcgtgt atgacctacc ggaacccgag ctgagaggag gagcgacgat
     9181 gcgatcagtt gtctgccgtg cggtcgtggt ggttgcggtt ggggtgggcc tcggcctcgc
     9241 cggcgcggcc tgggctcaga cgccctggac cgcgcccgaa tccgagaagg gcaagaagag
     9301 cccggttgcc gccagccaca aggtggccga gcagggcaag aaggtggccg aggtcaactg
     9361 cgtctcctgc cacgggaaga gtggcaaggg tgatggcgcc gcggcgatcg cgctgaaccc
     9421 caagcccgcc gattggacct cgaagaagat ccaggaggag agcgacggcg aggtcttctg
     9481 gaagatcacg accggtcggg gcgccatgcc ggcctggcgg catctcccgg agagcgaccg
     9541 ctgggcactc gtccagtaca tccggacgct caagaagtag aaccgggtcg tctgcaccag
     9601 aaaaatgagg catccgccgg atgccctgag ggcgatgggc gtctagggta ggggcagccg
     9661 aacagctgat tggcgggagg ttcggagtgg cgatcggcga gcggtacgag ggcatcctga
     9721 acgcggcgtg cagcgtgatc gcgcgccgcg gcttccatca ggcgtcgatc cgggagatcg
     9781 cccgcgcggc cgggctctcg ctggccggcc tctatcacta cgtcggcgag aaggacgagc
     9841 tgctgttcct ggtgctcggc cggtcgctgg accggctcct cgagagcctg ggccggaatc
     9901 tcgcggaggc gcccacgccc gagggccgcc tgctggccct catccagacc cacctcgact
     9961 tcgggtttca ccatgcccag gcgctccgga tcatcaaccg cgatcacgag ctgctcgcgg
    10021 agccgcggcg cgccgagatc gcggccaagc ggcaggccta cctgcaatgt gggctgggcg
    10081 tgctgcgcga gctcgacccg catgggcggt cggacgccga gctcttctcg gccacgaacc
    10141 tgctgctggg catgctcaac ggcatcgcca cgcgcccctt cctccggtct gccgatgacg
    10201 cacgcgccct cgcggccgag gtcggtgcgc tcttcctcca cgggttcctg gagcgggcgg
    10261 gccgcgtcga agagccgtcc tccttcgccc tgaggaggcc cgcatgacgc cttcgctgat
    10321 cctggagcgc tgtcgagacc tgatcgccca gccgctcgga gccgtggcgg agcgctggaa
    10381 ggccgagcat ccgggcggcc gggcggtggc ggcctacccg gtgtgggcgc ccgccgagct
    10441 ggtccacgcg gcgggcatgc tgccgctgtc gctcctgggc ggaggcacgt cggtggagct
    10501 gacccacgcg gacgcgcgct ttcagtcctt cgtgtgctcg attgccaagt cgagcctcga
    10561 gctcggcttc cagggcctgg tcaagggcgt cgacggcttc gtgttctcca acatctgcga
    10621 cgtggcccgc aacctgagca gcatctacaa gcggaacttc cccgacacct tcgtcgagta
    10681 cctgcacctg ccccagaact ccacctcgcc cgcggtgata ggatacgcgg cggccgagct
    10741 ccgccgcctc ggggcgtgct tcgatagcgc gttcggcctc gcggcgaccc cggccgcgct
    10801 cgccaagagc atcgagatct acaacgccct gcgcgcgcgg ctccgggccc tctacgggct
    10861 gaggatcgcc gagccccaga aactctccac ggccgagctc tatgtcgtgc tccgggccac
    10921 gaccctggcg cccccggaag aggccatcgc ctggctcgac gtcttgctgg acgccctccc
    10981 gcgccgcgag agccgcccgc gcgaccggct gcgcgtggtc gtggaaggcg ccttctgcga
    11041 gcagccaccc ctcgggctcc tcgaggtcct ggaggaggcg ggctgctacg tggtcgagga
    11101 cgacctgctg ctcggctggc ggtggttcac gtcggacgtg gcgagcgacg gcgatccgtt
    11161 cgagcgactg ggcgcggcct acgtgaacca ggcggtcccg tcgtcgaccc ggcacgaggg
    11221 tcgccagcac cggtccgagg gcctgatcga gaaggtgcgg cgggcgcgcg ccgacgcggt
    11281 cgtcttcatg cccgccaagt tctgcgagcc ggcgctgttc gactacgtgc tgatgaagca
    11341 ggggctggag cgggcgggga tcccgcatct cctcgtcgag ttcgaggaga agatgtggac
    11401 cttcgagcgc acgcgcaacg agatcgagac gttcgtcgag tccatgctgt tcgagtaatg
    11461 gcagggcgaa gtgagccgaa ggcgagccga gcggatacag gtaatggcag ggcgaagtga
    11521 gccgaaggcg agctgagcgg atacaggtaa tggcagggcg aagtgagccg aaggcgagct
    11581 gagcggatac cggaataaag gccgacagca ctacggaggc ggcaatgaca ggcgtgccgg
    11641 tcaccgaacg ccagtaccag ggacaggccc acgagcactc gcgccgcctc atgaccgcct
    11701 ggatggagga gctggcccgg gccgaggccg aggggcagcc caccggcgcc ctgatgatct
    11761 ccgggaactg cgtggagctc ctgcgcgcct gtcatatcct gcccatgttc ccggaggtga
    11821 ccgcgctcca gaacgcgatc cgcaagaagt cgctcccgct catcctcaag gccgagcagg
    11881 ccgggtactc cagcgacaac tgcgcctacg tcaaggccga catcggcctc ttcctcgacg
    11941 gcggcatggg gcccggcaag cccatcccgt tccccaccat cacggtctgc aactacgtgg
    12001 gctgcaacgt ctacgtgaag tggttcgagc acctggccga catcagcggg agcaagctct
    12061 tcatgttgga tgtcccgttc gcccggacgg ccgagccgag tccggccgac gtgcagtacg
    12121 tggtcgccca gctccgcgag ctcatcgcgc tctgcgagag tgtgtcggga aagaagttcg
    12181 acatcgacga gctgcgcgag atcctggccc acgccgctcg ggccgagggc ggctacgcgc
    12241 gcgccaagga gctctgcaag cgccacccgg cccccttcga cgcctacttc gacgccatca
    12301 acatgatggg cccgatcaac gtgcttcgcg gcacgcggga cgcggcggag ttcttcgaca
    12361 ccgcggtggc cgagttcgaa gggctcgtcg cccagggtct cgggccgctc ggcgaggagc
    12421 gcttccgcac cgtggtggaa ggccccccgc cctatccgtt ctacaagaac ttccggaatc
    12481 tcttcgccaa gtggggcgcg gtggcggtgg cctccacgta ctcgaccgtc ggcggcatct
    12541 gggagttcgg gttccggcac gacccgcggc ggccgctcga gtccatcgcc gagcagatgc
    12601 tgcgcgagaa cctcacgaac cgctccatcg tggcgcgcta tggccagatc aagcgctacg
    12661 tggaagagtg ggaggccgac gcgctggtca tccactccat caagtcctgc cggctgttct
    12721 cggccgggca gggggacatg cgcgactact tcacccagac gctcggcgtt ccgaccctga
    12781 tggtggagtc ggatctggag gatccccgct actacgccga ggcccagctc aagaaccgca
    12841 tcgacgcctt cttcgaatcg ctcgagcaca agaagctcac tgccgggcga gccgcagccg
    12901 caggcgaggc gagcgaatat gaaaagcgcg cgtagcgcaa ccggcgaggc ccgcctgcag
    12961 aagatcccgg tgtactgcta ccagtgcgtg gcgggacccg acctgctccg ggtcgtggtc
    13021 aaggacgggg tggccgtcgg cgtcgagccc aaccccgaga tggccgacgt ccacccggcg
    13081 tgcgggacgg tgtgcgtgcg cgcctacgcc ttgatccaga agctctacaa tccggcgcgc
    13141 atccagaccc cgatgcggcg caccaacccg cgcaaggggc gcgatcagga tcccggctgg
    13201 gagcccatca cctgggaaga agcgctcggc accctggcgg cccggctcaa tcgtctccgc
    13261 gcctcgggac tcgtggacgg ggccggctat ccgcggctgg cggtgaccat gggctcgggc
    13321 ggcatcgccc cggcctacct cggcaccctc gccgcgttcc tggcggcctg gggcccggtg
    13381 gaccagggca tcggcagcgg ccagggtgtc aagtgctacc actccgagca cctgtacggc
    13441 gagttctggc accgggcctt cacggtggcg gcggacgtgc cacgctgtga tttcgtcctc
    13501 tcgttcggct acaacggcga cgcgtccggc ggagtcaccg gcgtgtaccg gcacgccgag
    13561 gcgcgggcgc gcggcctcac ctgggtgcag ctcgagccgc acctgtccgt gacgggcgcg
    13621 ggagcgaccg agtgggtgcc gatccggccg aagacggacg cggcggtgct cttcgcgctg
    13681 ctccacgcga tcctgctcga gcacgactgg cgcgccgttt gcgacgtgcc gttcctcgag
    13741 cgcatgacga gctcgccata cctcgtcggt cccggcgggt actacctgcg cgacccgaag
    13801 acgctcaagc cgctgatctg ggaccagggc aaggaccgcg cggtcccctt cgacgatccc
    13861 gactgcgtga ggccggcgat ggagggcgag tacatcgcgg cgggcgtcga gatcggcgcc
    13921 gacggcgcgc ggcggagcgt gagcgagcgg gtgcggcccg ccttcgcccg cctcatcgag
    13981 cacgtgaagc cctacacgcc ggagtgggcg gccgtcatcg cggacgttcc cgccgagacc
    14041 atccggcggc tggcgcacga gtacgtgggg catgcgcggg tcggcgccac catcgccatc
    14101 gagggcgtca agtacccgca ccggccggtg gccgtactgc tcggcaagac cgtgaccaac
    14161 ggctggggcg gctacgagtg ctgctgggcg cggacggtgc tggccgcgct ggtgggggcc
    14221 ctcgaggtcc ccggcggcat cctcgggacc acggtgcggc tcaaccggcc cgcccagaat
    14281 cgccaggact ccgtgaggcc gggccccgac ggcttcatgg agcagccgct caacccgacc
    14341 gagcgaggca agtggcaggg cgcgccccac atccgcaacg cctatcgcac gctggtcccg
    14401 ctggccgaca actcgccgtg gtccgccgcg ctcggccccg cgcatctgcc gtggctgttc
    14461 atggagaacc cgcccgaggc ctggccggcc cctaccgtgc ccgacgtctg gatcatctat
    14521 cggacgaacc ccgcgatctc caactgggac accgggcgca tcgagcgcca gctcgagcgg
    14581 ttcccgttcg tcgccgcgtt cgcctacacc caggacgaga cgaactggta cgccgacctg
    14641 ctcctgccgg aagccacgga tctcgagtcg ctgcagctct accggatcgg cggcacgaag
    14701 tacatcgagc agtactggca gcacacgggc tgggccctcc ggcagcccgt cgtcgagccg
    14761 cccgtcgcca cccgcgacct caccgacatc gcgaccgagc tggctgcgcg cacgggcctc
    14821 ctggaggaat acctggccgc gctgaaccgg gggagcggaa ccacggtgcc gctcaagggc
    14881 gacgactacg actactcctt ccgcgagccg gtccgaccga tggccgacga gatctgggac
    14941 cgggtgtgcc gcgccgccac cgtgtcgctg agccgcggcg cggtggagcg cgatctggag
    15001 tggttcaagc gccacggcgc tttcttcgtg ccgttctcga gcaagcaata cttcctgcac
    15061 gggaccatgg tggcgcaggg gctgcgctac gagctgccct accaggagcg gatcaagcgg
    15121 gtgggcgagg agctgggagc gaggctgcac gagcgcggca tcaagtggtg ggacgtgcag
    15181 ctcgacgagt accagccgct gccgggctgg aaggactttc cgggcatctg gcaggagccg
    15241 gctcgcctca agggacgggc gcccggggag tttcccttct ggctcctgac cagccgcagc
    15301 atgcagtatt cctggggctc caacgtctcg ctgccggtcc tcgccgacgt cgcccgtcac
    15361 gtcaaggggc acttcggcgt gatgctcaat cgcggcgccg cccagcggct cggcatcgcc
    15421 gacggcgacc tggtcgagat cgagtcgccc accgggcgca cccagggacg cgccgtcctc
    15481 cgcgagggcg tgcgccccga cgtggcggtg gtcctccagc agttcgggca ctgggccacg
    15541 cccttcgcgc gcgatctcgg gatgccaaac ctcaaccagg tggcgagcat ggatctcgcg
    15601 ctcaccgacg ccaccggcag cggcgccgat ctggtcccgg tggccatccg gccggcaccg
    15661 ggactgcgcg agaccgccgc gcccaccgag gccgggttcc ggatgcccgt gccgctccga
    15721 cacccccgga tggatctcga gatctgcgag atgtacatga gggggctctg atgaccaggt
    15781 ggggaatggc catcgatgtg aaacgctgcg tgggctgcca gacctgcacg atcgcctgca
    15841 agcaggagca cggcctgccc gccgacaccg cgtggcgctt cgtcgccgat tgcgaggtgg
    15901 gggagtaccc cgacgtgcgg cgcctcttcc tgccgatgca gtgcatgcac tgcgccgagc
    15961 cgccctgcgt gccggtctgt ccgaccgggg cctcccgcca gcgggacgac ggcatcgtgt
    16021 gggtgcacta caacgcctgc gtgggctgcg gctactgcgc gatggcgtgc ccgtaccagg
    16081 cgcgccacct gatccactcc gcggacggct acttcgagag cccgacgccg tcggaggcgg
    16141 cccagacccg gccggagcgg cacggcgtga tgaccaagtg caccttctgc aaggaccggg
    16201 tggatgacgg gctcgcccac gggctgcggc ccggtgtcga tcccgacgcc acgccgatgt
    16261 gcgcgctggc ctgcatcgcc ggcgccatcg tcttcggcga tctggacgat gccacgagcc
    16321 gcgtctgcca cctcgccgcg gatccgcgcg cccaggtcct catgcccgag tgcggcaccg
    16381 agccgcgcgt gtactacctg gcggactgag cgatggccac cgcgatgacg gcgcagcgca
    16441 tggagatgat gccggcccgg cggcaggagc tctggggatg gccggcggtc gtcaacttcg
    16501 ccttgggcgg gctcggggcc ggctggtacg tcgttgcggt gctcgaggcc gggttcgagc
    16561 gatcgccggg cgtgaccgcg gcctcgtggg cggcgtccgc gctcgtgctg gccggcttcg
    16621 ccgcggtggc cgccgaggcg ggccgtccgc tgcgcgggcc gcgtgtgctg acgcggctcc
    16681 gcacgtcgtg gatgtcccgc gagctgctgg ccggcatcgc cttcgtcctg ctggtcgccg
    16741 ccgatctggc ctttccactt cgcctgtacc gggccccggc ggtggtcgcg gcggtcctgc
    16801 tggcgctcgc ccagggcttc atcgtgaggc gggcgcgcgg ggtcacggcc tgggacgtgc
    16861 cgctcatgcc gctgctgttc ctcctctccg cgctcgtctc gggggccggc gcgtatctgc
    16921 tggcagaggt cgccgcgggg cgcccgccgc tccccgcgct ggtgggagcc gtgctggcgc
    16981 tcgtggtcgt cgcgttcgtg gcctgggccc gctatctcgc ctggacgtcg gaggacacgt
    17041 accgacgggc cgttgccccg ctccgagagg gccgcgcggc cctgctcatc gacggcgccg
    17101 gctacggtct gccgctcctg ctcgggctgc tggcgctcgc cgcgccggcg gtggccgggc
    17161 ccctgacggg gctcgccggc acgctgctca tcgcgggcca ggcctacgcc aaggcgcggc
    17221 tcatccgggc ggcaggacgc ctccgcccca tcacgctcgg catcacgctc tcgcggagga
    17281 ggtcagcatg atctacggta ccggcgtcga cgtgggctcc acccagacca aggcggtgat
    17341 cgtggatgag agccgccgca tcgtggcgcg cgcgctgatc gccaccggcg ccaatgtctc
    17401 gcgcgcgggg gagaacgcgt tcgtccgcgc ctgcgagagc gccggcctgc cgcgggaggc
    17461 ggtgggctac gtggtgggca ccggctacgg ccgctacaag gtgacgttcg gcgacgccca
    17521 gatcacggag atcacctgtc acgcgcgcgg ggcccagtcg ttgttcccgg ccacgcggac
    17581 ggtgatcgac atggggggcc aggacaccaa ggcgatcaag gtggggccgg acggctcggt
    17641 ggtcgatttt tcgatgaacg acaaatgcgc ggccgggacg gggcgctttc tgtccgcggc
    17701 ggcggacgtc accggcgtgc cgctcgacga gatcggcccc ctgtcgctcc aggcgaagac
    17761 gccggtgcgg ctcacctcgg tctgcacggt cttcgtcgag tccgacatca tgtcgtatct
    17821 ggcccagcgc aagacggtcg aggacatcct cggcggcgtc cacaaggcca tcgcgacccg
    17881 gaccatgtcg ctggttcgcc gcgtggggat cgaggacgag atcacgttca ccggcggtgt
    17941 gtcgcgcaac accggcatgg tccgggcgct ggaggaggtg ctgggcaagc ccatcaacgt
    18001 gagcggcgag ggccactaca tgggcgccct cggcgccgcg ctgttcgcgc tggagcgcgc
    18061 gcaggcgggg gcccgcggcg aggcggtcgc gacgggcgcc gggaggagct agccatgctg
    18121 gtggccggga tcgacatcgg atcgggcacg accaagtgcg tgctggtgga cgagacgggg
    18181 acggtgcgcg ggcgcggcca gacgaagacc agggccgact tcgagaaggt ggcgcgggag
    18241 gcggttgcga cggccctcga cgccgcgggc ttccacggcg acgcgatcgg ctataccgcg
    18301 acgaccggcc tcggccgcta cgcggtgccc ttccgcgaca tccagatcac cgacctcacc
    18361 tgcggcgcgc gcggggcggc caccgcctgc ccggaggccc gcttcgtgct ggacatcggc
    18421 gcgcaatgct cgcgcgcgat ccgcctgcgc gacggcggca aggtcaagga gttccacatg
    18481 aacgagaaat gcgccgccgg gtccggcggc ttcctcgagc gggccgccaa gtacctcgag
    18541 gtcacgctcg ccgacatcgg cccgctgtcg cttcacgcgg cgcgccccca ggcgatctcg
    18601 agcgtgtgcg cggtgctggc cgagaccgag atcatcaacc acgtctccga gggcgtcgcg
    18661 gtcgagaaca tcctgcgggg catccacaac tccctggccg atcgcgcgtt gtcgctgctc
    18721 aagcgggtcg gtctcgacgg gccggtcgcc ttcatcggcg gcgtggcccg gcaggagggc
    18781 atgctggtgg ccctccggga caagctcggc gtgccggtgc acgtgccgga cgggccccag
    18841 ttcgccgccg ccctgggggc cgccctgctc ggccggcagc ggttcctcaa gacgggccga
    18901 cccgcggcgg cctgaggagc ggcgccatga gccggagcgc catcaacgtc cgccccgccg
    18961 agctgtcgga tcaggccgcc atcgtgcaga tcgacgagaa gctgtcgggc ctgacgcgga
    19021 aagactactg gcagcagcgc ctcgagatcg cggccctgcg gccgccctgg atgtcgctca
    19081 tcgcggagac cgacggccgg ccggtgggct ttctcttcgg ctgggtgggc gagtccgagt
    19141 tcggcatcgc ccggcgtacc gggtgggtgg acctcattgg cgtggccccg ccctatcgcg
    19201 gccgcggcgt cgcccaggct ctgctccgcc gcttcgtgga cagcggccgc gagctgcgcg
    19261 cgatcgagcg ggtggccacc ctcatcgacc tcggccagga agacgtgcgg gagttcttca
    19321 cccgggaggg atttcaccac ggccccatga tccacatgga gcgcgaggtg ctgccgtgag
    19381 gaccgtggcg agcccgacgc agaccctgcg cgaagagcac cgggtgatcc tgcgcgcgct
    19441 cgggctggtc gaggtggcgg ccgggcggct cgacgccggg cgagcgctgc cggccggctg
    19501 gtgggaggag ctgctcgagt ggctccgggc cttcgcggac cggaatcacc acgccaagga
    19561 agaggcgtat ctcttcccgg cgctggccga ggccggtgtt ccggccgccg gcgggcccgt
    19621 gggcgtgatg ctggacgagc acaccgaagg ccgcgggttg atccggctca tggccgagag
    19681 cccggtggag cggcgcgcgg cggccgcccg tcgctacgta cacctgctgc gcgaccatat
    19741 cgacaaggag aacggcgtgc tcctgccgct ggccgatgcc gtcctcgagc cgggggccca
    19801 gctaggcgtc gcccgcgcct tcgagcagat cgaggccgag caagggcccg cggcctccct
    19861 cgatcacgcg gagaccctgg tggaccggct cgccgcggcg ctgggagacc gagcttgaag
    19921 agctccctga tcgcgcccgg gaacccgggg tccggctacc cgaagagcga gaggccgaac
    19981 gcgacgatgg ccagcgccac caggacgttg atccgggcga cccaggacgc ccgcacccgc
    20041 gcggaagggt cggcgccggg cgcaccggcc cggggaccca ggacgaaatc gtggaacgcg
    20101 ctcaggatca gcgccagcac caccagcccg agcttccagt gaaagcgcgt ggaggacagc
    20161 aggacgggat gcatccacag gttcagcagg ccggtcacga tgagcacgcc gatcgcggcc
    20221 cagccgaccg cccggaagcg ccttcccgtg tcgcggatga gccgcgtacg gaggatcggg
    20281 tcctccagcc ggcgcgctat cggcaccatg acgagcgcga tgaagagcat cccgccgatc
    20341 caggtgatgg ccgagagcac gtgaagccag cggacgagga gcgacacgtc gggcaggata
    20401 gcacgtactc gcggtcggcc atctggacgg aacagaccgc ggcctggtac cgacgggccg
    20461 ccgaacgcgg agactacgcg gcgcgggtcc tcgaggccat cggctcccgg ctcgacctct
    20521 gcgggaccgc gctcgatgtg ggcgccgggt gcggagccct ggcgatcccg ctggcccgcc
    20581 ggctgcgctc cgtgaccgcg ctggagccgt cgcccgccat ggcccgcgag ctcaggcgat
    20641 gggcggtcga ggccgggctg gacaacatcg cggtggtgga gacggcgtgg ggcgagggcc
    20701 gggtgggtgc ccacgatctc gtgctctgcg cccatgtggg gaacctgttc cgcgcggagt
    20761 cggccttcct ccgcgaggtc gggcgggtgg cgcggcgcct cgtcgtcctg gtgcgggaca
    20821 ccacctccgc gagcggcggc gacaagttct tctacagcga gctgtacccg atcctgctcg
    20881 gccgtccgta cgcgacccgc ggtgacgccg acgacaccgt ggccgcgctc cggtctcttg
    20941 gtatccggcc cgcggtgacg gagatcgagt accgctccga ccagcccttc gccgacctgg
    21001 cggaggcgtg cgagttctgg atggcctatc tcgggctctc ggacgcggga tcccgcacct
    21061 acctcgccgg cttcctcggc gagcggctcg tccgttgcga cggagggtgg atcgccccgt
    21121 tccggaaatc tgctgcggtg atcacgtggg gaggctcatc atggctctag cgccgtccat
    21181 cataagagaa gagcaccgat cgctgtttgc cgtgatccac agcttccgga ccctggtccg
    21241 ggacgcacgc gatcgcgggc acgcgccgga ctacgagctg ttcacgctga tcctcgacta
    21301 catcgagggc ttctcggacc gctttcacca cccgaaggag gacgagcacc tgttcgcggc
    21361 gctccgtcgc cgctgcgccg aggcgaacga agcgctcgac gtcctggagc gcgaccacgc
    21421 gcgaggcgac gagctggtcc gggacctgcg gtatctcctg tcgcgctgcc gggtgggcgg
    21481 ggcggcggcg gtcgaggcct tcgcggcggc cgtcgaggag tacgtcgagt tctactggcg
    21541 ccacatgcgt ctggaggagg acgcgatcct gccgctggcc gagcggacgc tgaccgacgt
    21601 ggactggcag ccgatcgacg cggcgtttcg agcgaacgag gacccgctct tcggcgcccg
    21661 gccgagggag gagttccgga agctgttcca gctcatcgtg aacatggctc cggcaccggt
    21721 tggtgtaggt ccgagccccg ggacccgccc ggctccgtag gggcccgtat ccggcccgtt
    21781 gcgctcgagc ccgccggctg gggcgccggt gtccggggtg gggagccggc gccccggccc
    21841 tggggccgcc gggagcctcc accagccggg atttcggcag ttgagggcac ttggcggcta
    21901 ttcgtgctcg gggtcatcgc gccggcgcgg gcacccggtt tgcaacgacc cagcgacgag
    21961 ggccggtgga atggcccggt ctacccgacc cgatgacgga ggaggacgcg atgaaagctc
    22021 aggacagcat gacccacgac gtgatcacgg tcaccccgac gacgcccatc cacgaggtcg
    22081 cggccctcat ggccaatcac ggcgtgagtg gattgccggt cctcgacgag aaggggtccg
    22141 tcgtggggat cgtgaccgag gccgacctcc tcgtccggca aaagccccgc gcccgcatgt
    22201 cgtggtggag cctcttcctc gatggggagc ggatggcgcg cgaattccag cgcgcggtgg
    22261 ggaccaccgc cggcgaggtc atgacgcggg aggtgatcag catcggcccc gacctcccca
    22321 tcgaggccgc cgccgccatc ctccacgggc acgggattcg tcgcgtcccg gtggtggccg
    22381 acggcgtgct ggtcggcatc ctgagccgcg gggacctggt gaaggccctc gccggggcgc
    22441 ccccgcgcac cgccgcgccc gtctccgacg ctcggctcgt cgagacgatg aaggccggca
    22501 tggccgccga ggtgtgggcg ccccgcggca tcgtggtgga ggcggttgcc ggcgtcctca
    22561 cgctccgggg gctcgtgggc acccaggcag agtgcgcggc cctcgagacc atggcgcgct
    22621 cgattccggg ggttgcgggg gtggacaacc agctggtcgt caacccggta ctcccgtccg
    22681 gcgtgtgacg cggagaacgt gatgctgaac ggtggcgaga tcatcgtcga gtatctggcc
    22741 cgggagaagg ttccgtacgt cttcggcctc tgtggccacg gcaacgtggg gctgctcgac
    22801 gccctctacg accgcacgga cgccatccgg accatctcga cgcgtcacga gcagacggct
    22861 ggccacatgg ccgacgcgta tttccgcgtg gcgcaccgac cggtcgcgac cctgacctcg
    22921 tgcgggccgg ggtcggcgaa cctgcccatc gcgctggcct gcgccttcat ggactcgtcc
    22981 gcgctcctcg ccatcaccgg caatatcccg acctcccagt tcaaccgggc cgcgttccag
    23041 gagacgtacc ggcatttcca ggcggacttc ccggccgtca tccgcccgta cgtgaagcgg
    23101 agctggcagc ccacgcggac cgacatgctg cccaccgcgc tccggcaggc gttcgccacc
    23161 atgctgggcg ggcggcccgg ccccgtgaac ctcgacgtgc cgttcaacgt gttcaaggag
    23221 gaggcggagg tcgacgtccc ggagcccgga gcctggcgga ccgggatcag ctcccggagc
    23281 ggcggagagc cggacgcgat ccagcgggtg gtcgagctcc tgcgccaggc gcggcgccct
    23341 ctcatctacg tcggccacgg cgtgacgctc tcggaggcgg gggacgagct gaccgccttc
    23401 gcccgccgcc tgcggattcc ggtgatcttc tcccccaacg gctttggcgc gctggacatg
    23461 gcggatcctc tggcggtggg cttcgtcggg cgcaacggcg tctatcaggc caacgaggcc
    23521 gcgcggacgt gtgacgtgct gctggcgctc ggcgtccggt tcgacgatcg ctccagcagc
    23581 tcctggatcc cgggctactc gttcaatatt ccgcccaccc gcctcatcca cgtggacatc
    23641 gacggcgagg agatcggccg caactacccg gtgcatctcg gcatcgtcgg ggacgcgcgg
    23701 acggtgctgc gccagctcct ggccgccacc gagggctccg gagcggaggc gcccgcgcac
    23761 gacgcctggc gggcggacat cgccacgtgg cgcgctcagt gggaggagtt cgtgcgcccc
    23821 cacgagacga gcgaggccga gccgatccat ccgcaacgcg tggtccaggc actgcgcgcc
    23881 gtgatgccgg acgacgggat cgtcctcccg gacgtcggtg tgcaccacaa ctggatggtc
    23941 cagttctggc gggcgcggcg gccgcagacg ctgctcaaca cctggggctt cggggccatg
    24001 gggttcggcg tgtgcggcgt gctcggcgcc aagctggccg cgccggaccg gccctgcgtg
    24061 acggtgtgcg gcgacggcgg cttcatgatg cggccggacg tgctctgcac cgcggtggag
    24121 tacgacatcc ccgcggtctg ggtcgtctgg aacaacttcg cgtacggcgg catccgcgac
    24181 ctccagctcg gaatgttcgc gaagcgggag atcgcgacca gcttcgtccg ggagaaggac
    24241 ggccggctgg tgaacccgga cttcgtggcc ctggcgcgcg cgtgcggggc ggacggcatc
    24301 cgcgtcgagc gcccggccga cctggccgga gcgctcgagc acgccatcaa ggccgacgcg
    24361 ccgttcgtcc tggacgtgca cgtggatcgc gacatccggc cgcccggcgt ggggacgtgg
    24421 gagctgccgc cgttgcccta cggcgagccc gcgttcggca agcggcggct gccgcgcgag
    24481 ggcgagtgag ggagccgagc catggcgggc agagccgcgt gcaaggcaaa gatcatgcgc
    24541 atggaagagg ccttctggga gctgccgcct gggcacttcc aggccttctc gaagctcctg
    24601 gtgcatccga ccaacgccga gacgcgattc ttcgacttcc ggatttccag ctatcagccc
    24661 aggggatacg ccgaggtgca cgtccacgag gtggcggaga acctctatta catcctgcag
    24721 ggcaagggga tcgtggagct cgacggcgtg cgtcacgtgg tcgagcccca catggtcgtg
    24781 cacatcccgc ccggggtgcg gcacgggatc ttcaacacgg gtctggagga tctcgtcttt
    24841 ctggtggtgg cgtcaccgcc ggccgacatg ccgacggtct cgcagaccgg tggccgcgac
    24901 gtggaggccc ggtaacgcat ggcggggtac cctcgcctct tctcggccgg ccgcatcggc
    24961 tccctggcgc tcagaaaccg catcctcatg gcgcccatgg agaagaacct cgccctgccg
    25021 acgggcgcgg tgagcggacg gtacatcgac tactgcgagg cgcgggccgc cggcggggcc
    25081 gccctcatcc tgctcgaatc gatgtacgtg cacccggccg gcaagaacca tcgctaccag
    25141 ctgggcatcc acgatgacga cctggtgccg ggctaccggc ggctcatcga ggcgtgtcac
    25201 cgccatggag cgctcgtcgg ggcgcagctc cagttcgcgg ggcgtgagac ctcctcggcg
    25261 gtcacc
//
LOCUS       DASUFA010000290         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_398003, whole genome shotgun sequence.
ACCESSION   DASUFA010000290 DASUFA010000000
VERSION     DASUFA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398003"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFX87_10995"
     CDS             <1..689
                     /locus_tag="VFX87_10995"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4995491.1"
                     /translation="VLVVDRDEGLAKRTVEMITGEGGKASALAVDVTDEAHCAAMARE
                     AVSRYGRLDFLINNVGIGSKGSVVEEKPETWRRVMQVNVETMFLASKAAIPEMIRTAK
                     GGAIVNISSISALRPRGLTTYSASKGAVIALSKAMAVDHGPDGIRVNCIAPGPVYTPM
                     VYARGMTPAARDQRRRASVLAREGTGWDIGQAARFLLSGHARYITGQVLVVDGGATLV
                     GPARESLTEH"
     gene            728..1663
                     /locus_tag="VFX87_11000"
     CDS             728..1663
                     /locus_tag="VFX87_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4995492.1"
                     /translation="MRFGMFTSMGNQTWPAVLELWKHAEATRWDVACVTDHFMPNTKE
                     REGAMLEGWSTLSALSALVPRIQVGTIVLGNTYRHPAVVAKMAAQVDIISGGRLLLGL
                     GAGWQENEHEAYGIPFYTMRERLERLDEACQVMKSLWTENRSNFKGRYYTLSDAPLQP
                     KAVQKPHPELMIGGGGEKVTLLTVARYADHWNVWGGPKVLAHKGRILDEHCQRVGRDP
                     RAIRRSANMALLITDRKAEVDALAETIGSRMGRHAADARDTCLAGTPEQIRTTLAELR
                     AAKVDTLFIPTLFRPLPELRRDLDRFIAEIAPAFR"
     gene            1708..>3801
                     /locus_tag="VFX87_11005"
     CDS             1708..>3801
                     /locus_tag="VFX87_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX4995493.1"
                     /translation="MTRRRLLVTVAVALLATFVVSATAVWLALPSLARWAVVWQVEAQ
                     TGRKLTMRDFALDLRGGHLRITGLRLDDREPGPPLAELDRLEVRFRPGTLLRGHLWIE
                     DLALDNPRVRIVRTGRGVLNISDLLRPTKPRERAAAFTLDRFTLTGGAILFEDRTLTP
                     PRAWRADALTIEAALLSTVNPEPRGRGRLATTVAGARLSVDVSEVRLAPLHGQVHVNL
                     SNMDATLANLYLPPDTAVVLERALVSAGLTATVDAQDGLRLDGPARLDDLVVRRRGVD
                     ASLVTVPSLVFALTTVNGADGRPRGRVEVTGRATVHDPRPGQSHRFELERLKLVVDGL
                     DASGRAPAQVALTAALPGGGALDVQGTARPTPLGTELRARVSRVDLAFWLPYLELPLQ
                     FTGMAETDLTVDVAAAAGATSSTAGGGGVTTRVRGRATLNGATIYGPRAADGAGPGPG
                     AAPTTGSGSEGQTRGDRRRLLAADRMELTGLDAQWPKVRVERVRLARPRVTIERDREG
                     RLPILDILDSLKPAPAPTPTARPAAAPPASTFALEIGEVAVEEGRVLLDDSSVSPRAR
                     LRVTPIRLTATNVTWPSRGQARATLTATMPEAGTVQAEGSVALDPVRFDVRARMAGVA
                     LAPYRAYVRLPARLQGRLEADLTLKGQIGPQPMQLSARGTAALADLAFSDGDRPVLTV
                     GRIETVGLDYTWPAAA"
BASE COUNT          507 a         1483 c         1336 g          475 t
ORIGIN      
        1 cggtcctggt cgtggaccgc gacgagggcc tcgccaagcg caccgtcgag atgatcaccg
       61 gcgagggcgg gaaggcctcg gccctcgcgg tggacgtcac cgacgaggcg cactgcgcgg
      121 ccatggcgcg cgaggcggta agtcgctacg gccggctcga cttcctgatc aacaacgtgg
      181 gcatcggcag caagggcagc gtggtggagg agaagcccga gacctggcgg cgggtcatgc
      241 aggtcaacgt cgagaccatg ttcctcgcct ccaaggccgc gatccccgag atgatccgca
      301 ccgccaaggg cggcgccatc gtgaacatct cctcgatctc ggccctgcgc ccgcgcggcc
      361 tcacgacgta ctccgcctcc aagggcgcgg tgatcgcgtt gagcaaagcc atggcggtgg
      421 atcacgggcc cgacggcatc cgcgtcaact gcatcgcccc cggcccggtg tacaccccga
      481 tggtctacgc gcgcggcatg acccccgccg cgcgcgacca gcggcggcgc gcctccgtgc
      541 tcgcgcgcga gggcaccggc tgggacatcg gccaggccgc gcggttcctg ctctcgggac
      601 acgcccgcta catcaccggc caggtcctgg tggtggacgg cggcgccacc ctggtcgggc
      661 ccgcccgcga gtccctgacc gagcactgag cccggatcac gcccccggat cacccaagga
      721 gaccccgatg cgcttcggca tgttcacgag catgggcaac cagacgtggc cggccgtcct
      781 cgagctgtgg aagcacgcgg aggccacccg gtgggacgtg gcctgcgtga ccgaccactt
      841 catgcccaac acgaaggagc gcgagggcgc gatgctcgag ggctggagca cgctctccgc
      901 actctccgcg ctggtgccgc gcatccaggt cggcaccatc gtgctcggca acacctatcg
      961 ccacccggcg gtggtggcca agatggccgc gcaggtggac atcatctcgg gcgggcggct
     1021 cctgctcggg ctcggcgcgg gctggcagga gaacgagcac gaggcgtacg ggatcccctt
     1081 ctacacgatg cgcgagcgcc tggagcgtct cgacgaggcc tgccaggtga tgaagtcgct
     1141 gtggaccgag aaccgcagca acttcaaggg ccgctactac acgctctccg acgcgccgct
     1201 gcagcccaag gccgtccaga agccccaccc cgagctgatg atcggcggcg gcggcgagaa
     1261 ggtgacgctc ctcacggtgg cccgctacgc cgatcactgg aacgtgtggg gcgggcccaa
     1321 ggtgctggcc cacaagggcc ggatcctcga cgagcactgc cagcgcgtgg ggcgcgatcc
     1381 cagggccatc cgccgctcgg ccaacatggc gctgctgatc accgacagga aggccgaggt
     1441 ggacgcgctg gccgagacga tcggctcccg catggggcgg cacgccgcgg acgcgcgcga
     1501 cacgtgcctg gccggcaccc ccgagcagat ccggaccacg ctggccgagc tgcgggccgc
     1561 gaaggtggac accctgttca tcccgaccct cttccggccc ctgcccgagc tgcgtcggga
     1621 cctcgaccgc ttcatcgcgg agatcgcccc cgcgttccgc tagcctcccg ggtaggctca
     1681 gggccggcga caaggcatac tgagcccgtg acccgacgcc gccttctcgt caccgtcgcg
     1741 gtcgccctgc tcgccacgtt cgtggtgtcg gcgaccgcgg tctggctcgc gctgccgagc
     1801 ctggcccgct gggccgtggt ctggcaggtc gaggcccaga ccggccgcaa gctgacgatg
     1861 cgcgacttcg ccctcgacct gcgcggcggc cacctgcgca tcaccggcct ccggctcgat
     1921 gaccgcgagc ccggcccacc gctcgcagag ctggaccgtc tggaagtccg cttccgcccc
     1981 ggcacgctgc tgcgcggcca cctctggatc gaggacctgg ccctcgacaa tccccgcgtg
     2041 cgtatcgtgc ggaccgggcg cggcgtgctg aacatctccg acctgctgcg cccgaccaag
     2101 ccgcgcgagc gggcggccgc cttcacgctc gatcgcttca cgctgaccgg cggcgcgatc
     2161 ctgttcgagg accgcacgct gaccccgccc cgcgcctggc gggccgacgc gctcaccatc
     2221 gaggccgccc tgctctccac cgtgaacccg gagccgcgcg gccgtggccg cctggccacc
     2281 accgtggccg gcgcgcggct gtcggtcgat gtctccgagg ttcggctggc cccgctgcac
     2341 gggcaggtgc acgtcaatct gagcaacatg gacgcgaccc tcgccaacct ctacctgccg
     2401 ccggacaccg cggtggtact ggagcgcgcg ctcgtctcgg ccgggctgac cgccaccgtg
     2461 gacgcgcagg acggcctccg cctggacggc ccggcccgcc tcgacgatct ggtggtgcgc
     2521 cggcgcgggg tggacgcctc gctggtgacc gtcccctcgc tcgtcttcgc cctcaccacc
     2581 gtgaacggcg ccgacggccg accgcggggc cgcgtcgagg tcaccgggcg cgccaccgtg
     2641 cacgacccgc ggcccggcca gagccatcgc ttcgagctgg agcggctcaa gctggtcgtc
     2701 gacggcctcg acgcctccgg ccgcgcgccc gcccaggtcg ccctcaccgc cgcgctgccg
     2761 ggaggcggcg ccctcgacgt gcagggcacc gcgcgcccca cgccgctcgg cacggagctg
     2821 cgcgcgcgcg tgagccgggt ggacctggcc ttctggctcc cgtatctcga gctgcccttg
     2881 cagttcaccg gtatggccga gacggacctg acggtggacg tggccgccgc ggccggcgcg
     2941 accagctcga cggcgggggg cggcggcgtc acgacgcgcg tgcgcgggcg cgccaccctg
     3001 aacggcgcca ccatctacgg cccgcgggcg gccgacggcg cggggccggg tcccggcgcc
     3061 gcgcccacca ccggtagcgg gagcgagggg cagacgcgcg gggaccggcg acggctcctc
     3121 gccgccgacc ggatggagct gaccgggctc gacgcgcagt ggccgaaggt gcgcgtggag
     3181 cgcgtgcgcc tggcccggcc gcgcgtcacc atcgagcggg accgcgaggg ccggctgccc
     3241 atcctcgaca ttctcgactc gctgaagccc gcgcccgccc ccacgccgac ggcccgcccg
     3301 gccgccgcgc cgcccgccag caccttcgcg ctcgagatcg gcgaggtcgc ggtcgaggag
     3361 gggcgcgtgc tcctcgacga ttcctcggtg tcgccgcgcg cgcgcctgcg cgtcaccccg
     3421 atccggctca ccgcgaccaa cgtgacctgg ccgagccgcg gccaggcccg ggccaccttg
     3481 accgccacga tgcccgaggc cggcaccgtg caggcggagg gcagcgtcgc gctcgatccg
     3541 gtccgcttcg acgtgcgcgc gcggatggcg ggcgtcgccc tggccccgta ccgcgcctac
     3601 gtgcggctgc ccgcgcgcct gcaggggcgc ctcgaggcgg acctcaccct caagggccag
     3661 atcggcccgc agccgatgca gctctccgcg cgcggcaccg ccgcgctcgc ggacctcgcg
     3721 ttctccgacg gcgaccggcc cgtgctcacc gtcggccgca tcgagacggt cggcctcgac
     3781 tacacctggc cggccgccgc c
//
LOCUS       DASUFA010000291         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_511545, whole genome shotgun sequence.
ACCESSION   DASUFA010000291 DASUFA010000000
VERSION     DASUFA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511545"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFX87_11010"
     CDS             <1..192
                     /locus_tag="VFX87_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995494.1"
                     /translation="EDAALLARFVAAGRHRTPREVTAAAESEPAFSGARLTWLPFRRE
                     SRSLLDPSTGLALQEELLG"
     gene            200..682
                     /locus_tag="VFX87_11015"
     CDS             200..682
                     /locus_tag="VFX87_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995495.1"
                     /translation="MQSRRSWSAAGGAAPVARVVRVMLIVVLAAVVSVVGYHALRFVR
                     SCSTLDGAREAVEGHVRGKQVRRMARVLKTADREIVAARTAVRVTALTCGPSLLGGMT
                     CRTRYVVNGQSVGMEATDHYFRVTYSLLAGWQASSVTETSGLRYSLTPCRCSWAADGR
                     "
     gene            complement(634..1317)
                     /locus_tag="VFX87_11020"
     CDS             complement(634..1317)
                     /locus_tag="VFX87_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995496.1"
                     /translation="MDDINLLKGRMSEALVEGIFKRAQYMVARVARETQVSALMRTGQ
                     TVPDFVVWKALDEPSLGHRSYLLLAIEVKYCANLDDYLRRETPARSPEVALQWPELYE
                     ILVTERPELGRSCFQAVHVSILPPAAPPSTLDLHLVPGLDIYRRTVEEYEGLVREVFP
                     LIGSRNGGSQNGDWARKLGAWAPARHESRSDARIHQRSRPVGDHGSRPGDRGYRPSAA
                     HEQRHGVSE"
     gene            complement(1321..1740)
                     /locus_tag="VFX87_11025"
     CDS             complement(1321..1740)
                     /locus_tag="VFX87_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995497.1"
                     /translation="MIKRLATALLTALSLLVLPAGSLPAQTLDPAAAEALAATLRMLA
                     DPAARGQILATDPNAAAIDRQVQGMAGSSQLAQEVYGLAAEVFADLARSTGGDPRQLS
                     AALDRAKSDPAGFAAMLRPETLDRLRALATRISDAQR"
     gene            2010..>2174
                     /locus_tag="VFX87_11030"
     CDS             2010..>2174
                     /locus_tag="VFX87_11030"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4995498.1"
                     /translation="MPEPISHFVHVVRAGRLVFVSGCVASDDQGRTVGGSDIVAQTRQ
                     VHENIKRCLAA"
BASE COUNT          300 a          768 c          790 g          316 t
ORIGIN      
        1 gaggacgccg cgctgctcgc gcgcttcgtg gcggcgggcc ggcaccggac gccccgcgag
       61 gtgacggcgg ccgcggagtc cgagcccgcg ttcagcggcg cgcgcctcac gtggcttcca
      121 ttccggcgcg agtcccggtc actcctcgat ccgtccaccg ggctcgcgct ccaggaggag
      181 cttcttggct agacaacccg tgcaatcccg tcggtcctgg agcgccgccg ggggcgccgc
      241 tcccgtcgcg cgcgtcgtgc gcgtcatgct aattgttgtg ctcgccgcgg tggtctccgt
      301 ggtgggctat cacgcgctcc gcttcgtccg ctcctgctcc acgctcgacg gcgcgcgcga
      361 ggcggtcgag ggtcacgtcc gtggcaagca ggtgcggcgg atggcgcggg tgttgaagac
      421 cgccgatcgc gagatcgtgg ccgcgcggac cgcggtgcgg gtaaccgcgc tgacctgcgg
      481 gccgagcctg ctgggcggca tgacctgccg cacacgctac gtggtcaacg gccagagcgt
      541 ggggatggag gccaccgacc actacttccg cgtgacctac tcgctgctgg ccggctggca
      601 ggcgtcgagc gtgaccgaga cgtccgggct acgctactcg ctcacgccgt gccgctgctc
      661 gtgggccgcc gacggccgat agccgcgatc gccgggccgg gaaccgtgat cgccgaccgg
      721 ccgggaccgc tggtggattc gcgcgtcgct gcgcgactcg tgccgagcgg gcgcccatgc
      781 cccgagcttg cgtgcccagt ccccgttctg tgatccccca ttcctcgatc cgatgagcgg
      841 gaacacttcc cgcacgagcc cctcgtactc ctcgacggtg cgccggtaga tgtcgaggcc
      901 cggcaccaga tggagatcca gcgtgctcgg cggcgcggcc ggcggcagga tcgagacgtg
      961 gacggcctgg aagcacgagc ggccgagctc gggccgctcc gtcaccagga tctcgtagag
     1021 ctccggccac tgcagggcta cctcgggcga gcgggcgggc gtctcgcggc gaaggtagtc
     1081 atcgaggttg gcgcagtact tcacctcgat ggcgagcagc agatacgacc ggtggcccag
     1141 gctcggctcg tccagcgcct tccagaccac gaagtcgggc acggtctggc ccgtgcgcat
     1201 gagcgcggag acctgcgtct cccgggccac ccgtgccacc atgtactgcg cgcgcttgaa
     1261 gatgccctcg accagcgcct ccgacatccg gcccttcagc aggttgatgt cgtccatggt
     1321 tcagcgctgc gcgtccgaga tccgggtggc cagggctcgc agccggtcga gtgtctccgg
     1381 ccgaagcatt gccgcgaagc cggccggatc ggacttggcg cggtcgagcg ccgcgctcag
     1441 ctgccgcgga tcgccgcccg tgctgcgcgc gaggtccgcg aagacctcgg ccgcgaggcc
     1501 gtagacctcc tgggcgagct gcgacgagcc cgccatgccc tggacctggc ggtcgatcgc
     1561 agcggcgttc gggtcggtgg caaggatctg gcctcgcgcc gccgggtccg cgagcatccg
     1621 gagggtcgcg gcgagggcct ccgcggcggc cgggtcgagc gtctgcgcgg ggagggaccc
     1681 tgccgggagc acgagcaggg agagcgcggt gagcaaggcg gtggcaagtc gtttgatcat
     1741 gccgtccagg cggagcaaga gtcggaccac gcgcggaagt cggcaatcat cgagaaattc
     1801 cggagttggg ggcggaccgc ggcgctgcgc tgacggcccc tgtcacgcaa cggcgctttc
     1861 gtggacacgg cttgaccatc cggccgggcc ggagaataat cgccgcgcac ctccaccccc
     1921 agcgcgagga gagcacgatg cccacagccg ctcatcggcc cgcagctcgt aggcccgcag
     1981 ctcatcggga agagatctgg gtcccggaca tgccggagcc catcagccac ttcgtccacg
     2041 tggtccgcgc cggccggctc gtgttcgtct ccggctgcgt ggccagcgac gaccaggggc
     2101 gcacggtggg cggcagcgac atcgtggccc agacccgcca ggtccacgag aacatcaagc
     2161 gctgcctggc cgcc
//
LOCUS       DASUFA010000292         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_874436, whole genome shotgun sequence.
ACCESSION   DASUFA010000292 DASUFA010000000
VERSION     DASUFA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874436"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFX87_11035"
     CDS             <1..1017
                     /locus_tag="VFX87_11035"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX4995499.1"
                     /translation="AQRLAHFGRFAVDRMVAQQRRVLADYGVDFDVWTSERRDVRDAG
                     LADRAIEELAKRQLTYEDSEALWFRAAQYGDEKDRVVRKSDGELTYFAVDIGFHHFEK
                     FRQADHVIDFLGPDHHGHIPRMRAAMQALGNAPERFDVLIVQLVTLLRDGQPVRMSKR
                     RGEFVLMEELLEEVGRDAARFTFLTRRHDSPLEFDLAVATRQSSDNPVFYVQYAHARV
                     ASLFRNAAEQGATVPPWAEVDFSPLALDEEQALIKRLLQYPELVAGAARAREPHRVAY
                     YLSELAGLFHPYYKAHRVITADRAPTLARLGLCAAVGQVLRNGLDLLGVSAPDRMSRG
                     DERD"
     gene            1019..1747
                     /locus_tag="VFX87_11040"
     CDS             1019..1747
                     /locus_tag="VFX87_11040"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4995500.1"
                     /translation="MASSARRRVGGGPGGAQWMALGGAALVILGLTFALGLLVGRQWA
                     RQAPTLVVAGVTEAAKKPAAPPRRSGIAAETMADRAPESTEKLTFYKTLTEPLDGPAA
                     APKSEARPVAIKIPPATPSAQPPPAHGPAVTAKPAPAPPAAPPPSLPRAPAKPDSSAS
                     APVSAVTYTIQVGAYKNRRQADDSRQQLASAGLDAYVVTLAAQEGVARYRVRVGTYRS
                     REEAASAAERLRTQRSLTTFVTPK"
     gene            1759..2295
                     /gene="hpt"
                     /locus_tag="VFX87_11045"
     CDS             1759..2295
                     /gene="hpt"
                     /locus_tag="VFX87_11045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX4995501.1"
                     /translation="MDRRIGRILISRDEIAARVGELGEAIGRDYAGRAPVLVGVLKGA
                     VVFTADLLRAIPVPAAMDFMAVSSYGSGTRSSGVVRLTADLSLSIEGRDVIIVEDVID
                     SGRTISYLRRNLATRHPRSLALCGLLDKVSRREVDVDVDYVGFVIPDEFVVGYGLDYD
                     GRYRELPDLAVLEPVTPS"
     gene            2350..>3029
                     /locus_tag="VFX87_11050"
     CDS             2350..>3029
                     /locus_tag="VFX87_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX4995502.1"
                     /translation="MNPFYKNLALWMVIGLIVILLFQLFQQNQSPRGEIVFSEFLKKV
                     ETGEVREVTLKGNNVSGRLSDGSSFRTFTADYPDLVKSLKDRGVKIDVKPPETNSWLA
                     IVLQWVPMLLFIGVWIFFMRQMQGGGAKALSFGKARARLISEKQNKVTFQDVAGVDEA
                     KEELREIIEFLKDPPKFQKLGGKIPKGVLLVGPPGTGKTLLAKAIAGEANVPFFSISG
                     SDFVEMFVG"
BASE COUNT          431 a         1074 c         1069 g          455 t
ORIGIN      
        1 gcccagcgac tcgcgcactt cgggcgcttt gccgtggaca ggatggtcgc gcagcagcgg
       61 cgcgtgctcg cggactacgg cgtcgacttc gacgtgtgga cgtcggagcg acgggacgtg
      121 cgcgacgcgg ggctggccga ccgcgccatc gaggagctcg cgaagcgcca gctcacctac
      181 gaggacagcg aggccctgtg gttccgggcg gcgcagtacg gcgacgagaa ggaccgcgtg
      241 gtgcgcaagt cggacggcga gctgacctac ttcgcggtgg acatcgggtt tcaccacttc
      301 gagaagtttc gccaggccga ccacgtcatc gacttcctcg gacccgacca ccacggccac
      361 atcccgcgca tgcgcgcggc catgcaggcg ctcggcaatg cgcccgagcg cttcgacgtg
      421 ctcatcgtcc agctcgtgac cctgctccgc gacggccagc cggtgcggat gtccaagcgc
      481 cgcggcgagt tcgtgctgat ggaggagctg ctggaagagg tcggccgcga cgccgcgcgc
      541 ttcaccttcc tgacgcggcg gcacgacagc ccgctcgagt tcgacctcgc ggtggccacg
      601 cggcagtcct ccgacaaccc ggtgttctac gtgcagtacg cccacgcgcg cgtcgccagc
      661 ctcttcagga acgcggcgga gcagggggcg acggtgccgc cctgggcgga ggtggacttc
      721 tcgccgctcg cgctcgacga ggagcaggcc ctcatcaagc ggctcctgca gtacccggag
      781 ctggtggcgg gcgcggcgcg ggcccgcgag ccgcaccgcg tggcctacta cctgagcgag
      841 ctggccgggc tctttcatcc ctactacaag gcgcaccgtg tgatcacggc ggaccgcgcg
      901 ccgacgctcg cccgtctggg cctctgcgcc gcggtgggcc aggtgttgcg gaacggcctc
      961 gacctgctcg gcgtctcggc gcccgaccgc atgagccggg gcgacgagcg cgactgacat
     1021 ggcctcctcg gcgcggcggc gggtgggggg cggcccaggc ggcgcccagt ggatggcgct
     1081 cggcggcgcc gcgctggtga tcctgggcct caccttcgcg ctcgggttgc tggtcggccg
     1141 gcagtgggcg cgccaggcgc ccaccctggt ggttgcgggc gtgacggagg ccgccaagaa
     1201 gccggccgcg cccccgcgcc gcagcggcat cgcggccgag accatggccg atcgcgcgcc
     1261 ggaatccacc gagaagctca cgttctacaa gacgctgacc gagccgctcg acgggcccgc
     1321 ggccgcgccc aagtccgagg cacggccggt ggcgatcaag atcccgcccg ccaccccgtc
     1381 cgcgcagccg ccgcccgcgc acggaccggc ggtgacggcg aagccggcgc ccgcgcctcc
     1441 cgccgccccg cctccctcgc ttccgcgggc cccggcgaag cccgattcct cggcgagcgc
     1501 accggtttcg gctgtcacgt acaccatcca ggttggcgcc tacaagaacc gccgccaggc
     1561 cgacgactcg cggcagcagc tcgccagcgc cgggctcgac gcctacgtgg tcaccctggc
     1621 cgcgcaggaa ggggtggctc gctaccgggt gcgggtcggc acgtaccggt cccgcgagga
     1681 ggcggcgtcg gcggccgagc ggctccggac gcagcgctcg ctgaccacgt tcgtcacgcc
     1741 caagtaaggc cttcggcggt ggaccggcgc atcgggcgca tcctgatctc ccgggacgag
     1801 atcgcggccc gcgtcggcga gctgggcgag gccatcggcc gcgactatgc gggccgggcg
     1861 ccggtcctgg tcggtgtgct caagggagcg gtggtcttca ccgccgatct gctgcgggcc
     1921 atcccggtgc cggccgcgat ggacttcatg gcggtgtcct cctacggctc gggcacccgg
     1981 tcctccggcg tggtgcgtct caccgccgac ctgtcgctgt ccatcgaggg ccgggatgtc
     2041 atcatcgtgg aggacgtcat cgactcgggg cggaccatca gctacctgcg ccgcaacctg
     2101 gcaacccgtc atccccggag cctggccctc tgcgggctgc tcgacaaggt ctcccgccgg
     2161 gaggtggacg tggacgtgga ctacgtgggg ttcgtgatcc cggatgagtt cgtggtgggc
     2221 tacggcctcg actatgacgg ccggtaccgg gagctgcccg atctggccgt cctggagccc
     2281 gtcacgccct cgtgatcccg ctcggggcgc cccccgcgcg gcctcgcgcc ctatggtatg
     2341 attccctgca tgaatccgtt ctacaaaaac ctcgccctct ggatggtgat cggactcatc
     2401 gtcatcctcc tgttccagct cttccagcag aaccagtcac cccgggggga gatcgtcttc
     2461 tcggagttcc tgaagaaggt ggagaccggg gaggtccgtg aggtcacgct caagggcaac
     2521 aacgtctccg gacggctgag cgacggctcg tccttccgca ccttcaccgc cgactacccg
     2581 gacctggtca agtcgctgaa ggaccggggc gtgaagatcg acgtcaagcc gcccgagacc
     2641 aactcctggc tggccatcgt gctgcagtgg gtcccgatgc tgctcttcat cggcgtctgg
     2701 atcttcttca tgcgccagat gcagggcggg ggcgccaagg ccctgtcgtt cggcaaggcg
     2761 cgggcccggc tgatctcgga gaagcagaac aaggtcacgt tccaggacgt ggcgggcgtg
     2821 gacgaggcca aggaagagct gcgcgagatc atcgagttcc tgaaggaccc gcccaagttc
     2881 cagaagctcg ggggcaagat ccccaagggg gtgctcctgg tgggccctcc cgggaccggc
     2941 aagacgctcc tggccaaggc catcgcgggc gaggccaacg tgccgttctt ctcgatctcc
     3001 ggctcggact tcgtcgagat gttcgtggg
//
LOCUS       DASUFA010000293         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1651681, whole genome shotgun sequence.
ACCESSION   DASUFA010000293 DASUFA010000000
VERSION     DASUFA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651681"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFX87_11055"
     CDS             complement(<1..290)
                     /locus_tag="VFX87_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX4995503.1"
                     /translation="MAAQNLKFVNDNAFQLELRRRVDEFFLATGRRQRDCWQMYVKTA
                     IFLSGFAVSYWLLVFVAQAWWQGLLLAVLLGLSAAGIGFNIQHDGGHQAYSN"
     gene            complement(300..449)
                     /locus_tag="VFX87_11060"
     CDS             complement(300..449)
                     /locus_tag="VFX87_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX4995504.1"
                     /translation="MLWTIVVILLVLWALGMLSAYTAGGLVHLLLVVAIIVVVVQFVS
                     GRRSI"
     gene            complement(662..1783)
                     /locus_tag="VFX87_11065"
     CDS             complement(662..1783)
                     /locus_tag="VFX87_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995505.1"
                     /translation="MGSRLAAMIRKECSQLFRNVPILLFVIWAFTGAVYMGGHGMGAD
                     LRDYPVLVLDLSRSAESRELISRFRAPYFKIVGYAESDAEVVRGLDAGRASLAIVIPP
                     DFERRAHREGARFQVISDGSQSMSATIAGAHIASIAGRYSVELLEARLGGAAQARLPQ
                     VETRVRVEYNPNLVEAWFSGLLEVLNQVTMIAMLLAAAAMVREREHGTLEHLLVSPLR
                     PWELFAAKVVPVVALVPLAALGSVFGIVHGVFGTPIRGSVALFYVVSVIYVFALASLG
                     LAVAMLARNLGQAMLLLLLILYPMMLLSGAFTPPESMGPFMRYASLASPVRHYVDLGY
                     QVLFKGNGLAYVWPDVVGILALGVLLFAVSIRRFARLAR"
     gene            complement(1783..2928)
                     /locus_tag="VFX87_11070"
     CDS             complement(1783..2928)
                     /locus_tag="VFX87_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995506.1"
                     /translation="MRRLGGDRALAIARKETRELLRDPAYLGLAVVVPMLLILLLGHQ
                     LSLDVKGLLVAVVDRDRSRWSREYIDGMVNSEYFRLVGALDSAEEAREWIRSGRARVI
                     IDIPPDFGRRLSAGESATLGVTVDGSFPTRGQIGAGYVEAISALYNQRLLREYLARRG
                     AGDTTPWPVQMELAVWYNPTLESKNFIVPGMLVVILMIFPPLLSALLIVRERESGTIL
                     NLYCSPASRWDILAGKALPYVGVSFLDFLLIFAASIWLFRVRFVGAVWLLAVGALLYS
                     VCTVGLGLLISTLTRSQLAAMLATFLGAVAPAFTFSGIFTPAASLDAVGRFVSRLIPA
                     TYFMDVVRGSYLKGGGVVPYLPSLAMLMVYAVVIYAAAWLALRKRLG"
     gene            complement(2921..>3428)
                     /locus_tag="VFX87_11075"
     CDS             complement(2921..>3428)
                     /locus_tag="VFX87_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995507.1"
                     /translation="ALASAILHEPRVLFLDEPTAGVDPLARRQFWDLVHLLAHQHGVA
                     VLVSTHYMDEAAHCDRLGFMHEGRLVGLGRPSELTGRARAEGGPMLSVDASEFARAFT
                     LLRPRFPDAMLHGRHIRWQTRQPEADAQAARDALAAAGLAATVETRPLSMEDTFVSVL
                     RGAGLGRA"
BASE COUNT          610 a         1196 c         1202 g          420 t
ORIGIN      
        1 aaattcgaat aggcctgatg cccgccgtcg tgctggatat tgaacccgat ccccgcggcg
       61 gatagcccga ggagaacggc caggagcagg ccctgccacc aggcctgggc cacgaagacc
      121 aggagccagt acgacaccgc gaacccggac aggaagatgg ccgtcttcac gtacatctgc
      181 cagcagtccc gttgccgacg gcccgtggcg agaaagaatt cgtccacacg ccgccgcagc
      241 tcgagctgga aggcgttgtc attgacgaac ttcaggttct gggcggccac gaggtgtggt
      301 cagatggacc gacgaccgct cacgaactgg accacgacga cgatgatcgc gaccaccagc
      361 aggagatgga cgagcccgcc ggccgtgtac gcactcaaca tgcccagcgc ccacagcacc
      421 agaagaatca ccacgatcgt ccacagcatg gcagcctcct tcgagtaccg gccactgcat
      481 ccgtgccctg cccctccgca ttacaagctc gatgccaggc cggacgcaac gtcatttcag
      541 gaacttgcgt cgccggtccc cgtggcgcct ggggtgggcg cgccgcaaat ggggcggacc
      601 agccccagtc agccggcgcg gcgtgccggc ctaccgtcgg ccccctcgcc caccggatgg
      661 gtcagcgcgc gagccgcgcg aagcggcgga tggaaaccgc gaacagcagc acccccagcg
      721 ccaggatgcc caccacgtcc ggccagacgt atgccaggcc gttgcccttg aagaggacct
      781 ggtagcccaa gtccacgtag tggcgcaccg gggacgcgag gctcgcgtac cgcatgaacg
      841 gccccatgct ctcgggcggg gtgaaggcgc ccgagagcag catcatcggg tacaggatca
      901 ggagcaggag cagcatcgcc tggccgagat ttcgggcgag catcgcgacg gccaggccca
      961 ggctcgccag cgcgaagacg tagatcacgg agacgacgta gaagagcgcg acgctcccgc
     1021 ggatgggggt gccgaacacg ccgtggacga tgccgaacac cgagccgagg gcggccagcg
     1081 ggaccagggc caccaccggg acgaccttgg ccgcgaacag ctcccagggc cggagcgggc
     1141 tcaccagcag atgctcgagg gtgccgtgct cgcgctctcg caccatggcg gcggcggcca
     1201 gcagcatggc gatcatggtg acctggttca ggacctccag caggcccgag aaccaggcct
     1261 cgacgaggtt cggattgtat tcgacgcgga cgcgggtctc cacctgcggc agccgcgcct
     1321 gcgcggcgcc gcccaggcgc gcctccagca actccacgct gtagcggccc gcgatgctcg
     1381 cgatgtgagc cccggcgatc gtcgccgaca tggactggct gccgtcggag atcacctgga
     1441 accgcgcgcc ctcccggtgg gcgcggcgct cgaagtccgg cgggatgacg atggcgaggc
     1501 tggcgcggcc ggcgtcgagc ccgcgcacca cctccgcgtc gctttccgcg tagccgacga
     1561 tcttgaagta cggcgcgcgg aagcgcgaga tcagctcgcg gctctccgcg ctccgcgaga
     1621 gatcgagcac gagcaccggg tagtcccgca gatcggcccc catgccgtgc cctcccatgt
     1681 agaccgcgcc ggtgaaggcc cagatcacga acagcaagat cggcacgttc cggaagagct
     1741 ggctgcactc cttccggatc atggcggcga ggcggctgcc catcagccga gccgcttgcg
     1801 gagggcgagc caggccgccg cgtagatgac caccgcgtag accatcagca tcgcgaggct
     1861 cggcaggtac gggacgacgc cgccgccctt gaggtaactg ccgcggacca cgtccatgaa
     1921 gtaggtggcg gggatcaggc ggctcacgaa ccggcccacc gcgtccaggc tcgcggccgg
     1981 cgtgaagatg ccggagaacg tgaacgccgg agccaccgcg ccgagaaacg tggcgagcat
     2041 cgcggcgagc tggcttcgcg tcagcgtgga gatcagcagg ccgaggccca ccgtgcagac
     2101 cgagtagagc agcgcaccca ccgccaggag ccacaccgcg cccacgaacc ggacgcggaa
     2161 gagccagatg ctggccgcga agatcagcag gaagtcgagg aacgagaccc ccacgtacgg
     2221 caaggccttg ccggccagga tgtcccaccg gctggccggc gagcagtaga gattgagaat
     2281 ggtcccgctc tcgcgctcgc gcacgatcag cagggcgctc agcagcggcg ggaagatcat
     2341 gaggatcacc acgagcatgc cggggacgat gaagttcttg ctctccagcg tggggttgta
     2401 ccagaccgcc agctccatct ggaccggcca gggcgtggtg tccccggcgc cccgccgggc
     2461 caggtactcg cgcagcagtc gctgattgta cagcgcgctg atcgcctcca cgtagcccgc
     2521 cccgatctgg ccgcgggtcg ggaacgagcc gtccacggtc acgcccagag tggcggactc
     2581 gccggcgctg agtcgccgtc cgaagtcggg cgggatgtcg atgattacgc gggcccggcc
     2641 cgaccggatc cactcccgcg cctcctcggc gctgtcgagc gcgccgacga gccggaagta
     2701 ctcggagttg accatgccgt cgatgtactc ccggctccac cgggagcggt cgcggtccac
     2761 caccgccacc aggagcccct tgacgtccag ggacagctgg tggccgagga ggaggatcag
     2821 cagcatcggc accacgaccg cgagcccgag gtacgcgggg tcgcgcagga gctcgcgcgt
     2881 ctcctttcgc gcgatggcga gcgcgcggtc gccgccgagc ctacgcacgg ccgagcccgg
     2941 ctccccgcag cacgctgacg aaggtgtcct ccatcgacag cggccgggtc tcgacggtgg
     3001 cggcgaggcc cgcggcggcc agcgcgtccc gcgcggcttg ggcgtccgcc tcgggctgcc
     3061 gggtctgcca gcggatgtgg cggccgtgga gcatcgcgtc cgggaagcga ggacgcagga
     3121 gcgtgaaggc gcgggcgaac tccgacgcgt ccaccgagag catcgggccg ccctcggccc
     3181 gggcccgccc ggtcagctcg gacgggcggc cgagcccgac gaggcgccct tcgtgcatga
     3241 agcccagacg atcgcagtgc gcggcctcgt ccatgtagtg ggtggagacc agtaccgcga
     3301 ccccgtgctg gtgggccagc aggtgcacga gatcccagaa ctggcggcgg gcgagtgggt
     3361 ccacccccgc ggtcggctcg tcgaggaaca gcacgcgggg ctcgtgcagg atcgcgctgg
     3421 cgagggcg
//
LOCUS       DASUFA010000294         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_169867, whole genome shotgun sequence.
ACCESSION   DASUFA010000294 DASUFA010000000
VERSION     DASUFA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169867"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFX87_11080"
     CDS             <1..495
                     /locus_tag="VFX87_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX4995508.1"
                     /translation="ESGVADTADPLGGSYAVEAMTRRIEEEAETYIAKIDGLGGSVHA
                     IPFMQREIQEAAYRYQQDVEAKQRVVVGVNEFVMDEPPPANLFQVDATQSQAVAERLA
                     VLRRTRDAAAAARALEAVDRAARGRDNLLPPILTAVRAQVTLGEICDTLRQVFGVHQP
                     SVVF"
     gene            498..1307
                     /locus_tag="VFX87_11085"
     CDS             498..1307
                     /locus_tag="VFX87_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995509.1"
                     /translation="MRSIRWRQVIALSLFGVLLAGPAGAGTADQVGATFGLLLQDVVS
                     AFPAAEGLVVAAEGDRLFIDLTEKSGVQLGQEYSVFRKGEAFRHPINHRPLGRYEDIL
                     GYAQIVRLHPQYSEAVFVPAEGKATPEPEDGVRITKGRIRVAVAPATDLTKANADLRR
                     VPFMLAHALEQTKRFQVADPTLVQEHLLSQKTRSEELLVSPDKAKASGKTLEVSGWLI
                     PMLIERRGVTYLDITWISAVTGTALFSRRAALTRSENAAEQRFPWEPIPTD"
     gene            1365..>2011
                     /locus_tag="VFX87_11090"
     CDS             1365..>2011
                     /locus_tag="VFX87_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995510.1"
                     /translation="MTTYRRGRWLVLAGFIVIAVSGCGGRGWREYAGLAAVNDPSVNI
                     RPAEPKWLLIKNPRCCDIASEPEYIWVEEDKVPTTVKSFIFGKSTLIAPPEVVAKYGA
                     PPGGGRISARQGGAYKVENVVPTVPTGRSAAAAVVSNTTRTDAPGRVATQSATRGYVV
                     FVDTSRVVIDLAASDGVKLGTGVSVRRDKVAIVHPITGELLGELDEEVASGRVVEV"
BASE COUNT          299 a          704 c          719 g          289 t
ORIGIN      
        1 gagtccggcg tcgccgacac cgcggacccg ctcggaggct cctacgcggt ggaagccatg
       61 acgcgcagga tcgaggagga ggccgagacg tacatcgcca agatcgacgg cctcggcggc
      121 tcggtccacg ccatcccgtt catgcagcgc gagatccagg aggcggccta ccgctatcag
      181 caggacgtgg aggccaagca gcgcgtggtg gtgggcgtga acgaattcgt gatggacgag
      241 cccccgcccg cgaacctctt ccaggtggac gccacccaga gccaggccgt ggccgagcgc
      301 ctcgcggtcc tgcgccgcac ccgtgacgcc gccgccgccg cccgcgcgct cgaggccgtg
      361 gatcgggccg cgcggggccg cgacaacctg ttgcccccca tcctgaccgc ggtgcgcgcg
      421 caggtcacgc tcggcgagat ctgcgacacg ctgaggcagg tgttcggcgt gcaccagccc
      481 tcggtggtct tctagcgatg cggtcgatcc gctggaggca ggtgatcgcg ctgtccttat
      541 tcggtgtcct gctggcgggg ccggcggggg cgggcaccgc ggatcaggtc ggcgcgacct
      601 tcggcctctt gctccaggac gtggtcagcg cctttccggc cgcggagggc ctcgtggtcg
      661 cggcggaggg cgaccggctc ttcatcgatc ttaccgagaa gagcggggtg cagctggggc
      721 aggagtactc ggtgttccgc aagggcgaag ccttccggca tcccatcaac caccggcccc
      781 tgggccgcta cgaggacatc ctcggctacg cccagatcgt ccgcctgcat ccgcagtact
      841 cggaggcggt cttcgtgccg gccgagggca aggccacgcc cgagcccgag gacggggtcc
      901 gcatcaccaa gggccggatc cgcgtcgcgg tggctcccgc caccgacctc accaaggcca
      961 acgcggacct tcggcgggtg ccgttcatgc tcgcccacgc gctcgagcag acgaagcggt
     1021 tccaggtcgc cgaccctacc ctggtgcagg agcacctcct cagccagaag acgcgcagcg
     1081 aggagctgct ggtgagtccc gacaaggcca aggcctccgg caagaccctg gaagtctcgg
     1141 gctggctgat cccgatgctg atcgagcgcc ggggggtcac ctacctggac atcacctgga
     1201 tctccgcggt gaccggcacc gccctgttct cccgccgggc ggccctgacc cggtccgaga
     1261 acgccgccga gcagcggttc ccctgggagc cgatccccac cgactgagcc tccgcccgag
     1321 gcgcgggcgg atatgaggga gcttctggta tgctgttggg cgtcatgacg acctatcgac
     1381 gcgggcggtg gcttgttctg gccggcttca tcgtgatcgc ggtctcgggc tgcggcggcc
     1441 ggggctggcg cgagtacgcc gggctcgcgg cggtgaacga cccctcggtc aacatccgtc
     1501 cggccgagcc caagtggctc ctgatcaaga acccccgctg ttgcgacatc gccagcgagc
     1561 cggagtacat ctgggtcgag gaggacaagg tccccacgac cgtgaagtcc ttcatcttcg
     1621 gcaagtccac cctcatcgcg ccgcccgagg tcgtggccaa gtacggggca ccgcccgggg
     1681 gcgggcgcat cagcgcgcgg cagggtgggg cctacaaggt ggagaacgtg gtgccgacgg
     1741 tccccaccgg gcgcagcgcg gcggcggccg tggtgtcgaa caccacgcgc accgacgcgc
     1801 ccggccgcgt cgcgacccag tcggcgaccc gcggttacgt cgtcttcgtg gacacttccc
     1861 gcgtcgtcat cgacctggcg gcgagcgacg gggtgaagct cggcaccggc gtgagcgtcc
     1921 gccgcgacaa ggtcgccatc gtgcacccca tcaccggcga gctgctgggc gagctcgacg
     1981 aggaggtggc ctcggggcgg gtggtggaag t
//
LOCUS       DASUFA010000295         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_639107, whole genome shotgun sequence.
ACCESSION   DASUFA010000295 DASUFA010000000
VERSION     DASUFA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639107"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFX87_11095"
     CDS             <1..573
                     /locus_tag="VFX87_11095"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX4995511.1"
                     /translation="QLGQWVCQHYQYVSQFSRWCALVYGGCPDPDACDFLLENIIEEE
                     SGTKHVDLLIRFGEACGVSRTEVQSARQLPSTRGLTAWCYETARKPFHVAAAGLLVGL
                     ESQVPGIYRRNLPPLKTHYGFTDHEVEFFTIHIEADEIHGERGYQIVERHSTTPEKQR
                     EAAEAVREATEMRWQYMTGLHRAFVLKEDA"
     gene            570..881
                     /locus_tag="VFX87_11100"
     CDS             570..881
                     /locus_tag="VFX87_11100"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX4995512.1"
                     /translation="MSEPRQVASLDEVPAGRLKLVELDGTRIVLARIGDAVYACADVC
                     THRGGPLSEGKLNGSRLACPWHGWMYDVRTGQCLFPGRGASVPVYPVRVDEGAIFVEL
                     P"
     gene            878..1318
                     /locus_tag="VFX87_11105"
     CDS             878..1318
                     /locus_tag="VFX87_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX4995513.1"
                     /translation="MITVKRLTLEDALVLLAAAEAKAREIGVTETICVCDDGGHPIAL
                     HRMTGARITGVEIAIAKAFTAAGHRRATHKFNQPPAGPALPGNEAFGIHAMHPGRFAV
                     FVGGFPIEVDGDVVGGIGISGGNGEQDTAVGTAALEAFQRHVAR"
     gene            complement(1348..3210)
                     /locus_tag="VFX87_11110"
     CDS             complement(1348..3210)
                     /locus_tag="VFX87_11110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4995514.1"
                     /translation="MRKLPAAILPAGIFLVTCAAFWPALQGDFNWDDDINLVANLRFR
                     GFDASQIGWMFTNTLMGHYMPFTWLTFALDHALGGMAPWGYHLASLLLHAANAVLVYL
                     VARRLLTVALETTADIRVGAALAALLFALHPQRVESVAWISDRATLLCGTFYLLAVLA
                     YLRAVGLPDGRRRMWWGAASLAAFTAALLSKGMAMSLPITLLILDVYPLRRWRAGWPR
                     VIVEKLPYCALALVGAVVAAWARSQGAEFSDYGGYGITARIGLIAYSVCFYPLKLVWP
                     ADLSPLYEVPSQVRLLDPRFLIPLAALLAVTGALLALRRRAPGALAAWAHAAAFVAPV
                     SGVVHSGVQMVADRYSYLAQVGFVFLAGYGLVRLLELRRTGQVRRGAMAVAGSGIVLM
                     LGALAVLTWSQSYVWRDPETLWRWAVDLDPLCSRCHNNLGVALMHHRRDPQGLSESEE
                     HLREAVALRPTYALSHLNLGTVALLRGRYVEAEAALRSYVARQPDAPDGAERLAVLYL
                     AQGRSDEAIPLLRRARGITQSDGARADLPTAVELIRDGETLRFLGQALLERGRADDAI
                     VPLARAVRLYPSASSFRVWLAHAYRGAGQVALADGELAALRRLDPKAATPTTVR"
BASE COUNT          525 a         1145 c         1181 g          418 t
ORIGIN      
        1 cagctcggtc agtgggtgtg ccagcactac cagtacgtct cgcagttctc gcgctggtgc
       61 gcgctggtgt acgggggctg cccggacccc gacgcctgtg acttcctgct cgagaacatc
      121 atcgaggagg agtccggcac caagcacgtg gatcttctca tccgcttcgg cgaggcgtgc
      181 ggggtgagcc ggaccgaggt gcagagcgcg cggcagctgc cgagcacccg gggcctgacc
      241 gcctggtgct acgagacggc ccgcaagccc ttccacgtgg cggcggccgg gctgctggtg
      301 gggctcgagt cgcaggtgcc gggcatctac cggcgcaatc tcccgccgct caagacccac
      361 tacgggttca ccgaccacga ggtcgagttc ttcacgatcc acatcgaggc cgacgagatc
      421 cacggcgagc gcggctacca gatcgtggag cgccacagca ccacccccga gaagcagcgc
      481 gaggccgcgg aggccgtgcg cgaggcgacc gagatgcgct ggcagtacat gaccggcctc
      541 caccgcgcct tcgtcctcaa ggaagacgcg tgagcgagcc ccggcaggtc gcctcgctgg
      601 acgaggtgcc ggccgggcgg ctgaagctcg tggagctcga cggcacccgg atcgtgctcg
      661 cgcggatcgg cgacgcggtc tacgcgtgcg ccgacgtctg cacgcatcgc ggcggcccac
      721 tgagcgaggg caagctcaac gggagccgac tcgcctgccc gtggcacggt tggatgtacg
      781 acgtgcgtac cggccagtgc ctctttccgg gccggggcgc gagcgtgccg gtctatccag
      841 ttcgcgtgga cgagggcgcg atcttcgtgg agctgccgtg atcacggtca agcggctcac
      901 cctggaggac gcgctcgtgc tgctggccgc cgccgaggcc aaggcgcgcg agatcggggt
      961 caccgagacg atctgcgtgt gcgacgacgg cggccacccg atcgcgctcc accgcatgac
     1021 cggcgcgcgc atcaccggcg tggagatcgc gatcgccaag gccttcaccg ccgccgggca
     1081 ccgacgggcc acccacaagt tcaaccagcc gcccgccggg ccggccctgc ccggcaacga
     1141 ggccttcggc atccacgcca tgcatcccgg ccgcttcgcg gtcttcgtgg gcgggttccc
     1201 catcgaggtg gacggcgacg tggtcggcgg catcggcatc agcgggggca acggggagca
     1261 ggacaccgcg gtcggcaccg ccgcgctgga ggcgttccag cgccacgtgg cgcgctgaag
     1321 cccccgcgcg atcggcgggg gagcggacta gcggaccgtg gtcggcgtgg ccgccttcgg
     1381 atccagtcgc cgcagagcgg ccagctcccc atccgccagc gccacctgcc ccgccccacg
     1441 gtacgcgtgc gcgagccaga cgcgaaagct ggacgccgag ggatagagtc gcaccgcgcg
     1501 tgcgagcggg acgatcgcgt cgtcggcccg gccccgttcg agcagagcct gcccgagaaa
     1561 gcggagggtc tccccatcgc ggatcagctc caccgcggtc gggaggtcgg cccgagcgcc
     1621 gtcggactgg gtgatgccgc gggcccggcg caggagcggg atcgcctcat cggatcgtcc
     1681 ctgggcgaga tagagcacgg cgagccgctc ggctccgtcg ggagcatccg gctgcctcgc
     1741 cacgtagctc cgcaaggcgg cctcggcctc gacgtagcgt ccgcggagca gcgcgacggt
     1801 gccgaggttg aggtggctca gcgcatacgt gggccggagc gccaccgcct cgcgcagatg
     1861 ctcttccgac tcgctcaggc cctgcgggtc tcgacggtgg tgcatgagcg ccacccccag
     1921 attgttgtgg cagcgggagc agagcggatc cagatccacc gcccacctcc agagcgtctc
     1981 ggggtctcgc cacacgtagc tctggctcca cgtcaacacc gcgagggcgc cgagcatcag
     2041 cacgatcccg ctgcccgcca ccgccatcgc gccgcgccgc acctgacccg tccgccggag
     2101 ctcgaggagg cgcacgagcc catagcccgc caggaagacg aatccgacct gggcgaggta
     2161 gctgtagcgg tccgccacca tctggacgcc ggagtgcacg accccgctga ccggcgccac
     2221 gaaggcggcg gcgtgcgccc aggccgccag cgcccccggg gcacgacggc ggagcgcgag
     2281 cagggcgccc gtcaccgcga gcagggcggc gagcggtatc aggaagcgcg gatccaggag
     2341 gcgtacctgg ctgggcactt cgtagagcgg ggataggtcg gcgggccaca cgagcttcag
     2401 cgggtagaag cagacgctgt aggcgatcag cccgatgcgg gccgtgatcc cgtagccgcc
     2461 atagtcgctg aactcggctc cctggctccg agcccacgcg gccaccacgg cgcccacgag
     2521 cgcgagcgcg cagtagggca gcttctccac gatcacgcga ggccaacccg cccgccacct
     2581 ccggagcggg tacacgtcga ggatgagcag cgtgatgggg aggctcatgg ccatgccctt
     2641 ggacaggagc gccgccgtga aggccgcgag ggacgcggcg ccccaccaca tgcggcggcg
     2701 gccgtcgggc aggccgacgg cgcgcaggta cgccaagacc gcgaggagat agaaggtccc
     2761 gcacagcagg gtggcgcggt cggagatcca cgcgacggac tcgacccgct ggggatggag
     2821 cgcgaagagc agcgccgcca gcgccgcgcc caccctgatg tccgcggtcg tctccagcgc
     2881 gacggtcagc agccggcggg cgaccaggta gacgaggacc gcgttggcgg cgtgcagcag
     2941 caggctcgcg agatggtacc cccacggggc catcccgccg agggcgtggt cgagggcgaa
     3001 ggtcagccac gtgaacggca tgtagtggcc catcaaggtg ttcgtgaaca tccacccgat
     3061 ctggctcgcg tcgaaccccc ggaatctgag gttcgccacc aggttgatgt cgtcgtccca
     3121 gttgaagtcg ccctggagcg cgggccagaa ggccgcacag gtcaccagga agatgccggc
     3181 cgggagaatg gcggcgggga gcttccgcat cgcaaaccca ttgtcgcccg gtcgattttt
     3241 ggggtcgatg ctttttcgac tcggttaga
//
LOCUS       DASUFA010000296         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1566337, whole genome shotgun sequence.
ACCESSION   DASUFA010000296 DASUFA010000000
VERSION     DASUFA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566337"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFX87_11115"
     CDS             <1..399
                     /locus_tag="VFX87_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4995515.1"
                     /translation="GVFNVTRAVVTHMIARRAGKIINISSAAGEKGGRGQTNYAASKG
                     AINAFTRALAVELAPRRITVNAVAPGVIDTDMSQAIRDGAGEEVKARILLRRFGTAAE
                     VAYAVWFLASRFADYITGEILHVDGGFKME"
     gene            415..834
                     /locus_tag="VFX87_11120"
     CDS             415..834
                     /locus_tag="VFX87_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX4995516.1"
                     /translation="MEITREEILAVYPQVAKIMADALGCDVEQVRLDASLIEDLNAES
                     IDFLDIVFNLERTFKVKIPRGKIIEDARGDLPEAAFEDKGHLTELGRKRLREYLSEVP
                     ESRFRSPMRVADIPKLFTPETFCKLVARGLRAQKPDA"
     gene            827..1663
                     /locus_tag="VFX87_11125"
     CDS             827..1663
                     /locus_tag="VFX87_11125"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995517.1"
                     /translation="MPDRFCAFSFVDRITALDAGRRARGRFAVPAGLPGFSPCLVAEA
                     VGQLASWVAMAQADFRRRPVAALSGETRILGEAAPGEILDLGVEVESCDGDAVVFGGW
                     ARVAARPLLELGRCVGPMLDLEDFDAPEAVREDFQTLRGPGAPPRRFPDIAAPDLALI
                     DRVAGQRLRATMRVPVSAPFFGDHFPRRPVFPGSLLLDAQIRLGIRLATETLSPGQRL
                     RPARVTDVKMRSFILPGQAVEIETGMHAATSAAAELTLSAMVDGKRVATGRMEVVSSG
                     PA"
     gene            1660..2958
                     /locus_tag="VFX87_11130"
     CDS             1660..2958
                     /locus_tag="VFX87_11130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX4995518.1"
                     /translation="MTGPPRRVAITGLGLVTPLGNDVAATWQALLAGRSGVAPITHFD
                     AGGFPVRIAAEVKQFDPRAAIDDRKLLKLANRSHALALTAAEEAFRDAGIRPKGGTAT
                     RWACVVGSGKTGLGFGELGEVHRHCAPDGHFHPDRLLTGGFAMDPLAFCRSQTGAGLA
                     LLAQRFGIRGYATSVHTACASGSQAVGTAMKLIRRGTADYVLAGGFDSMINPIGVASF
                     CLLGALSTDNETPERASRPFDATRNGFVLGEGAGLLVLEEWDAARRRGARIYAELAGD
                     GNSLSSYRITDSHPSGDGPIQAMRQALAMGGVKPDDVDYLNAHGTSTLMNDRSECAAV
                     RAVFGSSAGKIGVSSTKSLMGHLIAGAGAVEAAVCALAIHHGAMPVNANLTTPDPECD
                     LDFIRTRPREGRIRVALSNSFGFGGSNSCLALRNPDEVEG"
     gene            2955..>4060
                     /locus_tag="VFX87_11135"
     CDS             2955..>4060
                     /locus_tag="VFX87_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX4995519.1"
                     /translation="MSARVLVTGSGAICAAGTSPDEIWGAVREGRSAIRPIQQWDISG
                     WPATLAGEIRGMDPRALVEDRKVHKLLQRCDLLGLYAAGRALDQSGFIAHRDALEPPA
                     AATVNDRTGVYVGVGVGAYQNEYEFFPLLTAAKGDLGTFGRELAATVHPMWLLRSMPN
                     NVVCHVGIHYGLKGPNACVTNHSVSGSLAVAEAAAGLRAGEADRALAVGHDAPIEPQM
                     VLYYHQLGLLGADGLRPFDARRGGSVLGEGAAALLLETELASASRRAGVIGEFLGSGC
                     TTEAEGLLPIRADGDGLAGAIAMALDDAELRPAEIGMIVAHGSATRMSDASEALAIRR
                     VFGAAAPPVTAFKWAFGHPLAASGILDSVLALVAL"
BASE COUNT          564 a         1458 c         1444 g          594 t
ORIGIN      
        1 ggggtcttca acgtgacccg cgcggtggtc acacacatga tcgcgcgccg cgcgggtaag
       61 atcatcaaca tcagctcggc agccggcgaa aagggggggc gcggtcaaac caactatgct
      121 gccagcaagg gggcgatcaa cgccttcacc cgcgccctcg cggtggagct ggcgccgcgc
      181 cggatcacgg tgaacgccgt ggccccgggc gtcatcgaca ccgacatgtc gcaggccatc
      241 cgcgacggtg ccggcgagga ggtcaaagcg cgcatcctgc tacggcggtt cggcaccgcg
      301 gccgaggtcg cctacgcggt ctggtttctg gcctcccggt ttgccgacta catcaccggg
      361 gagattctcc acgtcgatgg cggcttcaag atggagtgag cggaaaggac aaccatggaa
      421 atcacccgcg aggaaatcct ggccgtctat cctcaggtcg ccaagatcat ggcggacgcg
      481 ctgggctgcg acgtcgagca ggtccgcctc gacgcgtccc tcatcgaaga cctgaatgcg
      541 gaatcgatcg atttcctgga catcgtcttc aacctcgagc ggaccttcaa ggtgaagatc
      601 cctcgcggca agatcatcga ggatgcgcgg ggggatcttc cggaggcggc gttcgaggac
      661 aagggccacc tgaccgagct cggccgcaag cgcctgcggg aatatctcag cgaggtcccc
      721 gagagccgct tccgctcgcc gatgcgggtg gcggatatcc cgaagctctt caccccggag
      781 acgttctgca agctcgtcgc gcgcgggctg cgcgcgcaga agcccgatgc ctgaccgatt
      841 ctgcgccttc tccttcgtgg atcggatcac ggcgctggac gcgggacggc gggcccgggg
      901 gcgcttcgcc gtccccgccg ggcttcccgg gttctcgccc tgcctggtgg cggaagccgt
      961 cggacagctc gcgtcctggg tcgccatggc ccaggctgat ttccgccggc gacccgtggc
     1021 ggccctgtcc ggagagacgc gcatcctcgg ggaggcggcg ccgggcgaga tcctcgacct
     1081 cggcgtggag gtcgagagct gcgacgggga cgccgtcgtg ttcggcggat gggcgcgcgt
     1141 cgccgcccgc cccctcctgg agctgggccg ctgcgtgggg ccgatgctgg acctggagga
     1201 cttcgacgcc cccgaggcgg tgcgggaaga tttccagacc ctccgcggcc cgggcgcgcc
     1261 gccccgccgc ttcccggata tcgccgcgcc ggatctcgcg ctgatcgacc gcgtcgccgg
     1321 ccagcggctg cgcgcgacca tgcgcgtccc cgtctccgcg cccttcttcg gcgaccactt
     1381 cccccggcgc cccgtcttcc cgggctcgct cctcctcgac gcccagatcc ggctggggat
     1441 acggctcgcg acggagaccc tgtctcccgg ccagcggctc cggccggccc gcgtgaccga
     1501 cgtgaagatg cgctccttca ttctccccgg tcaggcggtg gagatcgaga ccggcatgca
     1561 tgcggcaacc agcgcggcgg ccgagctgac cctctccgcg atggtggacg ggaaacgggt
     1621 cgccaccggg cggatggaag tcgtctcgtc ggggccggca tgacgggacc tccgcgccgc
     1681 gtcgccatca ccgggctggg tctcgtgacc ccgctcggga acgacgtcgc cgccacctgg
     1741 caggccctcc tggccggccg cagcggcgtg gcgccgatca ctcacttcga cgccggcggc
     1801 ttccccgtgc gcatcgccgc cgaggtgaag cagttcgacc cgcgcgccgc gatcgacgac
     1861 cgcaagctcc tcaagctcgc caaccgctcc cacgcgctcg cgctcaccgc ggccgaggag
     1921 gccttccgcg acgccgggat ccggccgaag ggcggcacgg cgacgcgctg ggcttgcgtg
     1981 gtgggaagcg gcaagacggg cctggggttc ggcgagctcg gggaggtgca ccgccactgc
     2041 gctcccgacg gccacttcca tccggatcga ctgctgaccg gcggcttcgc gatggatcct
     2101 ctggccttct gccgcagcca gaccggcgcc gggctggccc tcctggccca gcggttcggg
     2161 atccgcggct acgccacctc ggtccacacc gcctgcgcgt cggggagtca ggccgtgggg
     2221 accgccatga agctgatccg gcgcggcacc gccgactacg tcctcgccgg cggcttcgac
     2281 tccatgatca accccatcgg cgtggcgagc ttctgtctgc tcggtgccct ctcgacggac
     2341 aacgagacgc ccgagcgcgc cagccgcccc ttcgatgcca cgcgcaacgg cttcgtcctc
     2401 ggcgagggcg cgggcctgct cgtcctcgag gagtgggacg cggcccgccg gcgcggggcg
     2461 cggatctacg ccgagctggc cggcgacgga aactcgctca gcagctaccg catcaccgat
     2521 tcgcatccgt ccggtgatgg gcccatccag gccatgcgcc aggccctcgc gatggggggc
     2581 gtcaagcccg acgacgtgga ctatctcaac gcccacggca cgtccacgct catgaacgac
     2641 cggagcgagt gcgcggcggt ccgggcggtc ttcggctcga gcgccgggaa gatcggcgtg
     2701 agctcgacca agagcctcat ggggcacctg atcgccggcg cgggcgcggt cgaggcggcg
     2761 gtctgcgccc tggccatcca tcacggggcc atgccggtga acgcgaacct gacgacgccg
     2821 gacccggagt gcgatctcga cttcatacgc acccggccgc gggaagggcg gatccgggtc
     2881 gcgctgtcca attccttcgg cttcggcggc tccaatagct gcctcgcgct gagaaatccc
     2941 gacgaggtcg aggggtgagc gcccgcgtgc tcgtcaccgg gagcggcgcc atctgcgccg
     3001 ccggcacctc gccggatgag atctggggcg cggtgcgcga gggccggagc gccatccgcc
     3061 ccatccagca gtgggacatc tcgggctggc cggcgaccct ggccggtgag atcaggggaa
     3121 tggatccgcg cgccctggtg gaggaccgca aggtccacaa gctgctgcag cgctgcgatc
     3181 tcctcgggct ctacgcggcg ggccgggccc tcgaccagtc gggattcatc gcgcaccgtg
     3241 acgcgctgga gccccccgcc gcggcaacgg tcaatgaccg caccggtgtc tatgtcgggg
     3301 tgggagtcgg tgcctaccag aacgagtacg agttcttccc gctcctcacc gccgcgaagg
     3361 gcgacctcgg caccttcggc cgcgagctcg ccgccaccgt gcacccgatg tggctgctgc
     3421 ggtccatgcc caacaacgtc gtgtgccacg tgggcattca ctacggcctg aaggggccga
     3481 acgcctgcgt gacgaaccac agcgtgagcg ggtccctggc ggtggccgag gcggccgcgg
     3541 ggctgcgcgc cggagaggcc gaccgcgcgc tggccgtcgg ccacgacgcg cccatcgagc
     3601 cgcagatggt cctctactac caccagctcg gcctcctggg cgcggacggg ttgcggccct
     3661 tcgacgcgcg gcgcggcggc agcgtgctgg gcgaaggggc ggcggcgctg cttctcgaga
     3721 cggagctcgc gtcggcgtca cgccgtgccg gggtcatcgg cgaatttctg ggcagcggct
     3781 gcaccaccga ggcggagggg ctgctgccca tccgcgccga tggcgacggg ctcgcgggcg
     3841 cgatcgcgat ggccctcgac gacgccgagc tgcgcccggc cgagatcggg atgatcgtgg
     3901 cgcatgggag cgccacgcgg atgtcggatg cgtcggaggc gctggccatc cgccgggtgt
     3961 tcggggcggc ggctcccccc gtcaccgcgt tcaagtgggc cttcgggcat cccctggccg
     4021 cctcggggat cctggatagc gtgctggccc tggttgctct
//
LOCUS       DASUFA010000297         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_875972, whole genome shotgun sequence.
ACCESSION   DASUFA010000297 DASUFA010000000
VERSION     DASUFA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875972"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFX87_11140"
     CDS             <1..950
                     /locus_tag="VFX87_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4995520.1"
                     /translation="VSWAGPVMGLTAADCVLATLPLSHSFGINGALLAPILAGARVAI
                     VEPFTPPGALEGIARHGVTVFPGVATMFRRLLDTPALDPSGLKTLRIAVSGAAPCPWD
                     LAREWRERTGVRILRGYGMTELFRPISFRADDARDVAESIGRPMPGVELRIVDDAGRP
                     CPAGETGELWIRSPAVMEGYLEAPEETRAVLADGWFRTGDLATISSDGFVAIAGRKKE
                     LILRGGYSVVPGEVEAALLTHPAVAEAAVVGVPHPDLGEDVAAFVTLRAGASADPTAL
                     IAHCRERLAGYKYPRRVTVLDALPKSATGKVLKARLTIG"
     gene            complement(937..1302)
                     /locus_tag="VFX87_11145"
     CDS             complement(937..1302)
                     /locus_tag="VFX87_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995521.1"
                     /translation="MHSTARGILDGLIVAVAGATAGYGVTFVIKHHAPAFFLLWIPSG
                     AIIAFLLARWKPRQWLIAGLATPALVLAETVARSPKAGPTAAALSLAFGLGWAGLTFV
                     GALAVRLGRTGYSRALNRS"
     gene            complement(1315..2070)
                     /locus_tag="VFX87_11150"
     CDS             complement(1315..2070)
                     /locus_tag="VFX87_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4995522.1"
                     /translation="MAGPTSSASSPPVPPAAKTELTPTGALRVGINYGNVLLATKDPA
                     TGEFRGVAPDLARELARRLGTPIGWVGYDSAGKMAEAVKVGGWDIAFLGAEPARAGEI
                     AFSPAYLEIEATYLVPAGSPIRSVAEVDREGIRIAVADKAAYELYLTRTLQRAKLVRA
                     PSLAASYELFVNEKLDALAGLKTFLLAEAPKIPGSRVLEGRFTGVQQAIATPRPREAA
                     AQYLREYVASVKASGLVAQAIERHRVRGVSVAP"
     gene            2153..>2409
                     /locus_tag="VFX87_11155"
     CDS             2153..>2409
                     /locus_tag="VFX87_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX4995523.1"
                     /translation="MKQQPLTAWHWKRSEYERLVDLGLFHGKPVELIGGQLLIAEPQG
                     SYHVTGISVAGDALRAVLPPGWYVRIQAPVALDEESEPELDL"
BASE COUNT          341 a          865 c          871 g          332 t
ORIGIN      
        1 tggtgtcctg ggcgggcccg gtgatggggc tcaccgccgc ggactgcgtg ctggccacgc
       61 tcccgctctc ccattccttc gggatcaacg gcgcgctgct ggctcccatc ctggccggcg
      121 cccgcgtggc catcgtggag cccttcacgc cgcccggcgc gctcgagggt atcgcgcggc
      181 acggcgtcac cgtgtttccc ggcgtggcca ccatgttccg gcgcctgctc gatacgccgg
      241 ccctggaccc gtccgggctc aagaccctcc gcatcgccgt ctcgggagcc gccccctgcc
      301 cctgggatct cgcccgggag tggcgcgagc gcaccggcgt gcgcatcctg cgcggctacg
      361 ggatgaccga gctcttccgc cccatctcct tccgcgccga cgacgcgcgc gacgtggccg
      421 agtccatcgg ccggcccatg cccggggtgg agctgcgcat cgtggacgac gcgggccgcc
      481 cgtgtcccgc cggcgagacg ggcgagctct ggatccggag ccccgccgtc atggagggct
      541 atctcgaggc gccggaggag acccgcgccg tcctggccga cggctggttc cgcaccggcg
      601 atctcgcgac catctccagc gacggcttcg tcgccatcgc ggggcggaag aaggagctca
      661 tcctgcgcgg aggctattcc gtggtgccgg gcgaggtgga ggccgcgctg ctcacccacc
      721 ccgccgtggc cgaggccgcc gtggtcggcg tgccgcatcc cgatctcggc gaggacgtgg
      781 cggcgttcgt gacgctgcgg gcgggggcgt ccgcggatcc cacggcgctg atcgcccact
      841 gccgcgagcg cctggccggc tacaagtacc cgcggcgcgt gaccgtgctg gacgcgctcc
      901 ccaagagcgc gacgggcaag gtgctcaagg cccggctcac gatcggttga gggcccgcga
      961 gtagcccgtt cggccaagac gaacggccag tgcccccacg aaggtcagcc ccgcccaccc
     1021 cagcccgaac gcaaggctca gagcggccgc ggtgggaccc gctttcgggg agcgggcgac
     1081 ggtctcggcc agcaccaggg cgggcgtggc gagcccagcg atcagccact gacggggctt
     1141 ccagcgcgcg agcaggaacg caatgatcgc gcccgagggt atccagagaa ggaagaacgc
     1201 cggggcgtgg tgcttgatca cgaaggtgac gccatagccg gcggtggcac cggcgacggc
     1261 cacgatcaat ccgtcgagga tgccgcgcgc cgtcgaatgc atgatcgcgc ccgatcaggg
     1321 cgccaccgac accccgcgca cgcggtgccg ctcgatggcc tgggcgacca gccccgacgc
     1381 cttcacgctc gcgacgtact cgcgcaggta ctgggcggcg gcctcgcggg gcctgggcgt
     1441 ggcaatggcc tgctgcaccc ccgtgaaccg gccctcgagc acgcgggagc cgggaatctt
     1501 cggcgcctcc gccaggagaa aggtcttgag gcccgcgagg gcgtccagct tctcgttgac
     1561 gaacagctcg tacgaggcgg cgaggctcgg cgcgcgcacc agcttggccc gctggagcgt
     1621 acgcgtcagg tagagctcgt aggccgcctt gtcggccacc gcgatgcgaa tgccctcgcg
     1681 gtccacctcc gcgacgctcc ggatcggcga cccggccggc accagatacg tggcctcgat
     1741 ttccagatag gcgggggaga acgcgatctc gcccgcgcgg gcgggctcgg ccccgaggaa
     1801 ggcgatgtcc cagccgccga ccttcacggc ctcggccatt ttcccggcgg agtcatagcc
     1861 cacccacccg atgggcgtcc ccaggcggcg cgccagctcg cgcgccagat ccggcgccac
     1921 gccgcggaac tccccggtgg ccgggtcctt ggtggcgagg agcacattgc cgtagttgat
     1981 gccgacccgc agcgcaccgg tgggcgtcag ctcggttttg gccgcgggcg gcaccggcgg
     2041 cgaggaggcg gaggaagtgg ggccagccat gggcgggact gtagcacgag attgggggtg
     2101 tctcgtcccg gcgacattgc gtgccggatg gggtcgagat agcctgacaa cgatgaagca
     2161 gcagcccctg accgcgtggc actggaagcg gagcgagtac gagcggctcg tggatctcgg
     2221 cctcttccac ggtaagcccg tcgagctgat cggcggtcag ctcctgatcg ccgaaccaca
     2281 gggcagctac catgtgacgg gaatcagcgt ggccggcgat gcgctacgcg ccgttctccc
     2341 gccgggctgg tatgtccgga ttcaggcgcc cgtggcgctg gacgaagagt ccgagcccga
     2401 actcgacct
//
LOCUS       DASUFA010000298         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1203010, whole genome shotgun sequence.
ACCESSION   DASUFA010000298 DASUFA010000000
VERSION     DASUFA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203010"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4765)
                     /locus_tag="VFX87_11160"
     CDS             complement(<1..4765)
                     /locus_tag="VFX87_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX4995524.1"
                     /translation="MPVGVRYVITLDADTRLPRGAVNRLVGTMAHPLNRPVFDTRAGR
                     VVDGYAILQPRITPTIPPDRDRSLFPRIFAGPAGIDPYATATSDVYQDLFGEGSYTGK
                     GIYDVAAFTAALKDRVPENSLLSHDLFEGIFARAGLVTDIELFEDFPTDYEVAAGRQY
                     RWARGDWQLLPWIVRGDTPAGARPTWIPLVGLWKITDNLRRTLSAPAAGLTLIVGWLL
                     PHGLPLVWTSFVLATIALPSLLPGFDEVIPKRSGISKRTHFRSVGRSFTIAAFQIALA
                     VTFLAHQAWLMADAIGRTLVRLYVTRRRLLEWTTAAQAKSDLSRDIGGAYRRMGGALL
                     LAAAAGTLIVLLRPSSLAVAAPFLLLWSLSPAVARWVSRPPAPSPQQRLSPADSAILR
                     ATARRTWRFFESFVGPADLHLPPDNFQEDPRPVLAHRTSPTNLGLYLLSVVSARDFGW
                     LGLLDTVDRLEATLATMRRLERFHGHFYNWYDTTGLRPLEPRYVSTVDSGNLAAHLLA
                     LGNACRAALHDPLPHRDLVAGIDDALQLVGEAATTLADGHRSQTVTVRQLAEARDTLA
                     SALREAPRGPAESVARVGRLAGHADTLVDVSRVLTTSGDGAEGGGVLVWAQAVRATIA
                     SHERDVETLMPWARHLEGAPSEVASTLTRLFSSPPSTADLPERCRVAIEEIAAARDGA
                     LRAGTVPPAALAPIDDLLESLARSAAAARALSGRLLAVARTTADLFQAMQFGFLFDPT
                     RKIFSIGYRVSDGSLDDSAYDMLASEARLASFIAIAKGDVPVSHWFHLGRQMTPVGLG
                     SVLVSWSGSMFEYLMPALVMGSPSGSLLEDTCRLVVQRQVSYGAANGVPWGVSESAYN
                     VRDLDLTFQYSNFGVPGLGLERGLSEDLVIAPYATALAAMIDPVAAARNFSALVAAGA
                     RGSYGFYEALDYTPSRVPDGATVAIVRAYMAHHQGMTLSALANVLYDGIMRARFHAEP
                     IVQATDLLLQERAPRDVAVARPRLEEVDVPAHVREFVEPVFRRFTSPHGPVPRTHLLS
                     NGRYAVMLTTAGSGYSRCGGLGVTRWREDVTRDPWGTYVFLRDVESGSVWSAAYQPTG
                     VEPAAYRATFSEDRAEFYRLDGTISTALEVIVSPEDDAEIRRVSVTNLGNGVRELELT
                     SYAELQLAPPSADSAHPAFSNLFVQTEFLADLGALLATRRVRSPAEPAVWAAHTVVVE
                     GQPAGGGQYETDRARFLGRGRTIRTPMSIIDGRPLSNTAGSVLDPIFSLRRRIRLAPG
                     ESARLIFSTLVAPSRESAVALADKYRDPATFERTVTLAWTQAQVQLHHLAIDPDEAHL
                     FQDLASRILYSDPAMRSAPDVLRRNTAGPSALWAHQISGDLPIILVRIDEPEDRGIVR
                     QLLRAHEYWRMKGLAVDLVILNEKAHSYVQEFQTSLETLVRASQSAPRPARSETEGRV
                     FILRKELLSPTDHAALQAAARAVLLSRHGTLAEQLARAEPPPAAAALPARRPVRAEPA
                     APASPPRLQSEFFNGIGGFLDGGREYVTVLGEGQWAPAPWVNVISNPGFGFQVSESGA
                     GYTWALNSRENQLTPWSNDVVGDPPGETIYVRDEET"
BASE COUNT          733 a         1524 c         1887 g          629 t
ORIGIN      
        1 cggtctcctc atcgcggacg tagatcgtct cgcccggcgg gtcgccgacc acgtcgttgg
       61 accagggggt gagctggttc tctcgactgt tgagggccca cgtgtacccg gccccggact
      121 ccgagacctg gaagccgaag ccggggttgg agatcacgtt gacccaggga gcaggcgccc
      181 actgcccctc gcccagcacg gtgacgtact cgcggcctcc gtcgaggaag cccccgatgc
      241 cgttgaagaa ctcggattgg agtcgtggag gcgacgcggg cgcggcgggc tccgcccgga
      301 ccggccgccg tgcgggaagg gccgcggcgg ccggaggcgg ctcggcgcgc gcgagctgtt
      361 ccgccagcgt gccgtggcgg ctcagcagca cggctcgggc ggccgcctgc agagccgcat
      421 ggtccgtggg cgagagcagc tccttgcgca ggatgaacac ccgaccctcg gtctcgctcc
      481 gcgcgggccg gggagccgac tggctcgccc ggaccagggt ctcgagcgag gtctggaact
      541 cctggacgta ggagtgcgcc ttctcgttca ggatgacgag atcgacggcc agccccttca
      601 tgcgccagta ctcgtgtgcg cgaaggagct ggcgcacgat gccgcgatcc tcgggctcgt
      661 cgatgcgcac gaggatgatc gggaggtcgc cggagatctg gtgggcccag agggccgacg
      721 ggcccgctgt gttccgccgc aggacgtccg gggcggagcg catcgccggg tcggagtaga
      781 ggatccggct ggcgagatcc tggaagaggt gggcctcgtc cgggtcgatg gccaggtggt
      841 ggagctgcac ctgcgcctgc gtccacgcca gggtgacggt gcgctcgaag gtcgcggggt
      901 cgcgatactt gtcggccagg gccaccgcgg actcgcggga cggagcgacc agcgtcgaga
      961 agatgaggcg ggcgctctcg cccggggcga gtcggatgcg acgtcgcagg ctgaagatcg
     1021 gatcgagcac cgagcccgcg gtgttcgaca gaggccggcc gtcgatgatg gacatcggag
     1081 tgcggatcgt gcggccccgg cccaggaagc gggcccgatc cgtctcgtac tggcccccgc
     1141 ccgcgggctg gccttccacc acgacggtgt gggccgccca gaccgccggc tccgccggtg
     1201 agcggacgcg gcgcgtggcc agcagcgcgc cgagatcggc gagaaactcg gtctggacga
     1261 agaggttgga gaaggccggg tgggcggaat cggccgaggg cggggcgagc tggagctcgg
     1321 cgtaggaggt gagctccagc tccctgaccc cgttgcccag gttcgtcacc gagacgcggc
     1381 ggatctcggc gtcgtcctcg ggcgagacga tcacctcgag ggcggttgag atcgtcccgt
     1441 ccagccgata aaattccgct cggtcttccg agaacgtcgc acggtaggcg gccggctcga
     1501 cgccggtggg ctggtacgcg gccgaccaga ccgagccgct ctcgacgtct cgcaggaaga
     1561 catacgtccc ccacggatct cgcgttacgt cctcgcgcca gcgggtgacg ccaaggccgc
     1621 cgcaccggct gtagcccgac ccggcggtgg tcagcatcac cgcgtagcgc ccgttggaga
     1681 ggaggtgcgt ccgggggacc ggcccgtgag ggctggtgaa gcggcggaag acaggctcca
     1741 cgaactcgcg gacgtgagcc ggcacgtcca cttcctcgag ccgggggcgg gcgaccgcga
     1801 cgtctcgggg agcgcgctcc tgcaggagca ggtcggtggc ctggacgatg ggctcggcgt
     1861 ggaacctcgc gcgcatgatg ccgtcgtaga ggacgttcgc cagcgcgctc agcgtcatgc
     1921 cctggtgatg cgccatgtag gcgcggacga tcgccaccgt cgcgccatcg gggacccgcg
     1981 acggcgtgta gtcgagggcc tcgtagaagc cgtaggatcc ccgagcgccc gcggcgacga
     2041 gcgccgagaa gttccgggcc gcggcgaccg ggtcgatcat ggccgcgagt gcggtcgcgt
     2101 agggggcgat gaccaggtct tcgctcaggc ctcgctcgag acccagcccc ggcacgccga
     2161 agttcgagta ctggaaggtg agatccagat cgcggacgtt gtatgccgac tcggagacgc
     2221 cccaggggac gccgttggcg gcgccgtagc tcacctgacg ctggaccacg agccggcacg
     2281 tgtcttcgag caggctcccc gacggggagc ccatcacgag cgcgggcatc aggtactcga
     2341 acatcgagcc ggaccaggac acgagcaccg agccgagccc gaccggggtc atctggcggc
     2401 cgaggtggaa ccagtgcgag acggggacgt cacccttggc gatggcgatg aagctcgcga
     2461 gccgcgcttc cgaggcgagc atgtcgtagg cgctgtcgtc caggctgccg tcggagacgc
     2521 ggtagccgat cgagaagatc ttgcgggtcg ggtcgaagag gaagccgaac tgcatggcct
     2581 ggaagaggtc ggcggtggtc cgggcgacgg ccaggaggcg cccgctgagc gctcgggcgg
     2641 ccgcggcgga gcgcgccagg ctctcgagga ggtcgtcgat gggcgccagc gcggccggcg
     2701 gcaccgtacc cgcgcggagg gcgccgtcgc gagcggcggc gatttcctcg atcgcgacgc
     2761 ggcagcgctc gggcaggtcg gcggtgctgg gcggcgagga gaagagccgg gtgagtgtgc
     2821 tggcgacctc cgaaggagca ccctcgaggt gccgcgccca tggcatcagg gtctccacgt
     2881 cgcgctcatg gctggcgatg gtggcgcgca ccgcctgggc ccagaccagg acgccgccgc
     2941 cctcggctcc atcgccgctg gtcgtcaaca cacgcgagac gtccaccagg gtgtcggcgt
     3001 gcccagcgag gcgcccgacg cgggcgaccg attccgccgg gccccggggg gcctcgcgga
     3061 gcgccgaggc cagggtgtcg cgggcctcgg cgagctggcg aacggtgacc gtctggctgc
     3121 ggtggccgtc ggcgagcgtg gtggcggcct ccccgacgag ctggagcgcg tcgtcgattc
     3181 cggcgaccag gtcgcggtgc ggaagcggat cgtggagcgc ggcccgacac gcgttgccga
     3241 gggcgaggag atgcgcggcg aggttgccgc tgtccacggt ggacacgtag cggggctcca
     3301 gcgggcgcag cccggtggtg tcgtaccagt tgtagaagtg gccgtggaag cgctcgagac
     3361 ggcgcatcgt cgccagggtc gcctcgaggc ggtccaccgt gtccaggagg cccagccagc
     3421 cgaagtcccg cgcggagacc accgacagca gatagaggcc gaggttggtc ggagacgtgc
     3481 ggtgcgccag aacgggccgg ggatcttcct ggaagttgtc gggcgggaga tggaggtcgg
     3541 cgggcccgac gaaggattcg aagaagcgcc aggtgcggcg cgcggtggcg cgcaggatag
     3601 cggagtccgc gggagagagc cgctgctggg gcgacggcgc gggcggccgg ctgacccatc
     3661 gggccaccgc cggggagagg ctccagagca gcaagaaggg cgcggcgacc gcgagactgc
     3721 tcggccgcag caggacgatc agggtgcccg cggccgcggc gaggaggagc gccccgccca
     3781 tgcgccggta cgcgccgccg atgtcccggg agagatccga cttggcctgc gcggccgtcg
     3841 tccattccag cagtcggcga cgagtcacgt ataggcgcac gagtgtgcgt cctatcgcgt
     3901 cggccatgag ccacgcctgg tgggcgagga acgtgacggc gagcgcgatc tggaacgccg
     3961 cgatcgtgaa gctccgcccg acgctccgga agtgggtccg cttggagatg cccgaccgct
     4021 tcgggatgac ctcgtcgaac ccggggagga gggacgggag cgcgatggtg gcaaggacga
     4081 acgaggtcca cacgagcggg aggccgtgag ggagaagcca gccgacgatg agggtgagcc
     4141 cggcggccgg cgccgacaag gtccggcgga ggttgtcggt gatcttccac agtccgacga
     4201 gggggatcca ggtcggccgc gcccccgcgg gggtgtctcc gcggacgatc caggggagga
     4261 gctgccagtc gccgcgtgcc cagcggtact ggcgtccggc cgcgacctcg tagtcggtgg
     4321 ggaagtcctc gaagagctcg atgtcggtga cgagtcccgc tcgggcgaag atcccctcga
     4381 agagatcgtg gctcaggagc gagttctcgg ggacgcgatc cttgagggcc gcggtgaacg
     4441 cggccacgtc gtagatgccc ttcccggtat acgagccctc gccgaagaga tcctggtaga
     4501 cgtcggaggt cgcggtggcg tagggatcga ttcccgccgg cccggcgaag atcctcggga
     4561 agagcgagcg gtcgcgatcg ggaggtatcg tgggcgtgat ccggggctga aggatcgcgt
     4621 agccatccac cacacggccc gcgcgggtgt cgaagaccgg ccggttcagc ggatgggcca
     4681 tcgtgccgac gaggcgattg accgcgccgc ggggcaggcg ggtgtcggcg tccagggtga
     4741 tgacgtagcg gactccgacc ggcacgcccg ccg
//
LOCUS       DASUFA010000299         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1111942, whole genome shotgun sequence.
ACCESSION   DASUFA010000299 DASUFA010000000
VERSION     DASUFA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111942"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFX87_11165"
     CDS             <1..1157
                     /locus_tag="VFX87_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995525.1"
                     /translation="MGGPAADVLARDGMIARIAPGIEPAAAATVVDGDNGILLPGLVD
                     AHMHLDKTFWGLPWRAHEAGPTTLERIENERRLRRELRLPADVQAERLVRQAVSRGTI
                     HIRTHVDVDTEIGLRNFEGVMATRSRLRDAVSIQVVAFPQSGVVTRPGTAELLERAVK
                     DGAELIGGVDPTGIDQDAKGQLDVIFGIAGRYGAGVDIHLHDPGEQGAAQIRMIAART
                     RALGLGGKVAVSHAFCLGMVDEPEVARLVDLLREERIAIMTTAPGDRPTPPVRRLREA
                     GVAVGAGSDGVRDAWTPFGNADMLERAMLIAYRNGFRTDALVHDALDVVTRGNAALLG
                     LARYGLAVGDRADLVVLPGETLGELVAMRPPRAWVISGGRVIARNGQCVL"
     gene            1189..1929
                     /locus_tag="VFX87_11170"
     CDS             1189..1929
                     /locus_tag="VFX87_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4995526.1"
                     /translation="MASISPAVRSDFAPTGKMRVGINHGNLVLAQKNATTGEVKGVAV
                     DLARELGRRLDVPVELVQYGSAGQMTDALATGAWDVAFLAREAGRAGEIGFTAPHLLI
                     EGTYLIPAGAPYRRVPDVDRAGVRIAVSEKSAYDHFLTRNIKHATLVRAPGNSAGVGL
                     VQGGKADVIAGVRQLLLMDQAKLPGSKVLDDRFMVIEQAMGLPKGRDAGLAYAKAFIE
                     EMKASGFVAQALEKSGVRGVPVAPPEKP"
     gene            complement(1943..2308)
                     /locus_tag="VFX87_11175"
     CDS             complement(1943..2308)
                     /locus_tag="VFX87_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995527.1"
                     /translation="MPKIWRVYSGADGQSHVEEMPLAMKPFTDTEGAHGEGTPMQSVA
                     GLAFRVAPPGYVLDWHCAPRRQYSITLSGQAEIEVGDGTVVRVGPGDVLLAEDLTGQG
                     HVTRAVGTTPRFYAIVPLA"
     gene            complement(2347..3084)
                     /locus_tag="VFX87_11180"
     CDS             complement(2347..3084)
                     /locus_tag="VFX87_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995528.1"
                     /translation="MKVAFVGATRGMGRALARALAERGDALFLLGRDAAEMALSARDL
                     EARGTRAPVGHAPLDLGRPAGFADALDAADRALGGLDALVVTGGAFGRQEELAADPAR
                     LATLLEVNFTGTALLCQQAATRLAAKGGGLICAFSSVAGDRARRSNYLYGASKAGLSV
                     FLDGLDLAYRERGVRVLCVKPGFVRTAMTAGLPVPPFAGEPEAVAAAVVRAMDRGRHQ
                     VYAPGIWWGIMLIIRALPRAVLRRVQF"
     gene            complement(3081..4421)
                     /locus_tag="VFX87_11185"
     CDS             complement(3081..4421)
                     /locus_tag="VFX87_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4995529.1"
                     /translation="MSELPLEELAGWGRHPVGRARVDRSGEPELPAGGEPVIARGLGR
                     AYGDAAMPATPGGLVVECTRADRILGFDGGTGRLTCEAGLSLAEILRVFVPRGWFPPV
                     TPGTKFVTVGACVACDVHGKNHHRDGSFGSFVERLVLVTADGRRVECGPALERELFLA
                     TVGGMGLTGLIVEVTLRLRRVESPWIVLETVGVEGLEAMLAGLKRSADDWPYTVGWID
                     CLAQGRAVGRGILMRGRHATPMEAAGRKLPRGLPLTVPVDAPEWLLNPLFMRWFNYLY
                     YRLHGGTLKRRVVSYDRFFYPLDAVGRWNRLYGRRGFLQYQCVLPRAAGAAPLVAVLA
                     RLGTAGAASFLAVIKDCGPASDAYLSFPMEGTTLALDLAYRGTASEALIHELNAVVIA
                     HGGRVYLAKDAVTRAEDFARMMPRLAEWRAVRDRWDPGHRFRSAQSVRLLGDRA"
     gene            complement(4418..>4714)
                     /locus_tag="VFX87_11190"
     CDS             complement(4418..>4714)
                     /locus_tag="VFX87_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995530.1"
                     /translation="MVAVVTASTVTAYALYTMSPETVAKFNTRLLPVTLPFVLYGVFR
                     YLYLLYRRDMGGNPSDLLLSDRALLVNTGLWMLALLVIIYGPVWGPTWLVRWAP"
BASE COUNT          697 a         1707 c         1735 g          575 t
ORIGIN      
        1 cgatgggcgg ccccgccgcc gacgtgctgg cccgcgacgg gatgatcgcg cggatcgccc
       61 ccggcatcga gccggcggcg gccgccaccg tggtcgacgg ggacaacggc atcctgttgc
      121 ccggcctcgt ggatgcccac atgcacctcg acaagacctt ctggggcttg ccgtggcggg
      181 cacacgaggc cgggcccacc accctcgagc gcatcgagaa cgagcggcgc ctgcgtcggg
      241 agctgcggct gccggccgac gtgcaggcgg agcggctggt gcgccaggcg gtgagccgcg
      301 gcacgatcca catccggacc cacgtggacg tggacaccga gatcggcctg cgcaacttcg
      361 agggcgtcat ggccacgcgg agccgcctgc gcgatgcggt ctcgatccag gtggtggcct
      421 tcccgcagag cggcgtggtc acgcgcccgg gcaccgcgga gctgctcgag cgcgcggtga
      481 aggacggagc cgagctgatc ggcggcgtgg atcccaccgg gatcgatcag gacgcgaagg
      541 gccagctcga cgtgatcttc gggatcgcgg ggcgctacgg ggcgggcgtg gacatccacc
      601 tgcacgatcc cggcgagcag ggcgcggcgc agatccgcat gatcgccgcg cggacccggg
      661 cgctcgggct cggcgggaag gtcgcggtga gccacgcctt ctgcctcggg atggtggacg
      721 agcccgaggt cgcacgcctc gtcgacctgc tccgcgagga gcggatcgcg atcatgacca
      781 ccgcgcccgg cgatcggccg acaccgccgg tgcggcggct ccgcgaggcc ggggtcgcgg
      841 tgggcgcggg ctcggacggc gtgagggatg cgtggacgcc cttcggcaac gcggacatgc
      901 tcgagcgcgc gatgctgatc gcctaccgga acggcttccg caccgacgcg ctggtccacg
      961 acgcgctcga cgtcgtcacg cggggcaacg cggcgctgct cggcctcgcg cgctacggcc
     1021 tcgcggtcgg ggaccgcgcc gacctcgtcg tgctcccggg cgagacgctc ggcgagctgg
     1081 tcgcgatgcg cccgccccgc gcctgggtga tcagcggcgg ccgggtcatc gcccggaatg
     1141 gacaatgcgt tctctgaacc cctcaacccg ctaggagcct gcgccaccat ggcatcgatc
     1201 tctccggctg tccgctccga cttcgccccg accggcaaga tgcgtgtcgg catcaaccac
     1261 ggcaatctgg tcctcgccca gaagaacgcc acgaccggcg aggtgaaggg cgtcgcggtg
     1321 gacctggcgc gtgagctggg ccgccggctc gacgtgccgg tcgagctggt ccagtacggc
     1381 tcagccggcc agatgaccga cgcgctggcg accggcgctt gggacgtcgc gtttctcgcg
     1441 cgcgaggcgg gacgggcggg cgagatcggc ttcaccgccc cgcacctgct gatcgagggc
     1501 acctatctga tcccggccgg ggcaccctat cgccgcgtcc ccgatgtcga tcgcgccggg
     1561 gtgcggatcg cggtctccga gaagagcgcc tacgaccact tcctcacccg caacatcaag
     1621 cacgccacgc tggtgcgcgc accgggcaac agcgccggag tcggcctcgt gcagggcggc
     1681 aaggccgacg tgatcgccgg cgtgcgccag ctgctgctga tggatcaagc caagctgccg
     1741 ggctcgaagg tgctcgacga ccgcttcatg gtgatcgagc aggcgatggg tctgcccaag
     1801 ggccgcgacg cgggcctcgc ctacgcgaag gccttcatcg aggagatgaa ggcctccggc
     1861 ttcgtggcgc aggcgctgga gaagtcgggc gtgcgcggcg tcccggtcgc gccccccgag
     1921 aagccctgac gcggactccg actcaggcga gcgggacgat cgcgtagaag cggggcgtcg
     1981 ttccgaccgc ccgggtgacg tgaccctggc cggtgagatc ctcggccagc agcacgtcgc
     2041 ccggccccac gcggaccacg gtgccgtcgc cgacctcgat ctcggcctgc ccggagagcg
     2101 tgatcgagta ctgccgccgc ggcgcgcagt gccagtcgag cacgtagccg ggcggggcga
     2161 cgcggaacgc gaggcccgcg acggattgca tcggcgtgcc ttccccgtgc gccccctcgg
     2221 tgtcggtgaa gggcttcatc gcgagtggca tctcctcgac atgcgactgc ccgtccgcgc
     2281 cgctgtagac ccgccagatc ttcggcatgc gatccctttc cgtgaggagc tccgtgagga
     2341 gtggcgctag aactgcacgc ggcgcaggac ggcgcgcggc agcgcgcgga tgatcagcat
     2401 gatcccccac cagattcccg gggcgtagac ctggtggcgg ccccggtcca tggcccgcac
     2461 gaccgcggcg gccaccgcct ccggctcgcc cgcgaagggc ggaacgggca gccccgcggt
     2521 catcgcggtc cggacgaagc cgggcttgac gcacagcacg cgcacgccgc gctcgcgata
     2581 cgccaggtcg aggccgtcga ggaagaccga gaggccggcc ttggacgcgc cgtagaggta
     2641 gttgctccgc cgggcgcggt cgcccgccac gctgctgaag gcgcagatca gtcccccacc
     2701 cttcgcggcc agccgcgtgg cggcctgctg gcagagcagc gcggtcccgg tgaagttgac
     2761 ctcgagcagc gtggccaggc gcgcggggtc ggcggccagc tcctcctgac ggccgaaggc
     2821 cccgccggtc accacgaggg cgtcgagacc gcccagggcg cggtcggccg cgtcgagcgc
     2881 atcggcgaac ccggccggcc ggcccaggtc gagcggagcg tggccgaccg gcgcgcgcgt
     2941 gccccgcgcc tccagatcgc gcgcggacag cgccatctcg gccgcgtcgc ggcccagcag
     3001 gaagagggcg tcgccgcgct cggcgagcgc ccgcgccagc gcgcggccca tgccgcgggt
     3061 cgcgccgacg aaggccacct tcacgcgcgg tctccgagga gccggacgga ctgggcggag
     3121 cggaaacgat ggcccggatc ccagcggtcg cgcaccgcgc gccactcggc caggcgcggc
     3181 atcatgcgcg cgaagtcctc ggcgcgcgtc accgcgtcct tggcgaggta gacgcgcccg
     3241 ccgtgggcga tcaccaccgc gttcagctcg tggatcaggg cttcgctggc cgtgccgcgg
     3301 tacgccaggt cgagcgccag cgtggtacct tccatcggga aggagagata ggcgtcggag
     3361 gcggggccac agtccttgat cacggccagg aacgaggccg cgccggccgt gccgagccgg
     3421 gcgaggaccg cgacgagcgg cgcggcaccg gccgcgcgcg gcaacacgca ctggtactgg
     3481 aggaagccgc gccggccgta gagccggttc caccgcccca ccgcgtcgag cgggtagaag
     3541 aaccggtcgt aggagaccac gcgccgcttg agcgtgccgc cgtgcagccg gtagtagagg
     3601 tagttgaacc agcgcatgaa gagcgggttc agcagccact ccggagcgtc caccggcacg
     3661 gtgagcggca acccgcgcgg cagcttgcgg ccggccgcct ccatcggggt ggcgtgccgc
     3721 ccgcgcatca gaatgccgcg gccgaccgcg cgcccctggg ccaggcaatc gatccagcca
     3781 acggtgtagg gccagtcgtc cgcgctccgc ttgagcccgg ccagcatggc ctcgagtccc
     3841 tccacgccca ccgtctccag cacgatccac ggcgactcca cccggcggag ccgcagcgtg
     3901 acctccacga tgaggccggt gagccccatg ccgcccaccg tggcgaggaa gagctcgcgc
     3961 tcgagcgcgg gcccgcattc cacgcgcctg ccgtcggcgg tgacgaggac caggcgttcg
     4021 acgaagctgc cgaaggaacc gtcgcggtgg tgattcttgc cgtggacgtc gcaggccacg
     4081 caggccccga ccgtgacgaa cttggtgccg ggcgtgaccg gcgggaacca gccgcggggc
     4141 acgaacacgc gcaggatctc ggcgaggctc agccccgcct cgcaggtgag ccgcccggtg
     4201 ccgccgtcga atcccaggat gcgatcggcc cgtgtgcact ccaccacgag cccgcccggc
     4261 gtcgccggca tcgcggcgtc gccataggcg cggcccaggc cgcgcgcgat caccggctcc
     4321 ccgcccgccg gcagctccgg ctcgcccgag cggtccaccc gagcgcgtcc caccgggtgg
     4381 cggccccacc ccgccagctc ctccaggggc agctcgctca cggcgcccat cgcacgagcc
     4441 acgtgggtcc ccagaccgga ccgtagatga tgacgaggag cgccagcatc cagaggccgg
     4501 tattgacgag gagcgcgcgg tcgctgagca ggaggtcgga ggggttgccg cccatgtccc
     4561 gacggtagag caggtagaga tatcggaaga cgccgtagag cacgaagggc agcgtcaccg
     4621 gcaggaggcg cgtgttgaac ttggcgaccg tctccggaga catcgtgtag agcgcgtagg
     4681 cggtcacggt ggatgcggtg accaccgcca ccat
//
LOCUS       DASUFA010000300        11786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1654347, whole genome shotgun sequence.
ACCESSION   DASUFA010000300 DASUFA010000000
VERSION     DASUFA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654347"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1193
                     /locus_tag="VFX87_11195"
     CDS             66..1193
                     /locus_tag="VFX87_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995531.1"
                     /translation="MGSRLLGIIVKEFIHLKRDMLALVLALAVPVAMLFIFGWAINTD
                     VKHIPTAVFEQSGSLEARLFLEAMENSQYFDVRRRVSSHKELTRLIDSGEAKVGVVIP
                     PDYARRLTRQDAAIQVIVDASDPQVATSALNAATSLGAQRSLQVLTQTLEGTRLARQP
                     EPPVDVRVRAWYNPDLVSAIFIVPGLIGALLMQTTITAMAVSVVREREKGTLEALIVS
                     PIRRWELLLGKIIPNLLVAFGQMTMALLVAHFVFDVPIRGSLPLLYVLSLVFMMGTLG
                     VGILLSTASRTVPQAMQLAFLTFLPSIYLSGLLFPIEGMPTGAQYLAAIIPLTYFLKI
                     VRGIILKGVGFEYLWPSLVPLMIFGTVVFSLAVLKFRKQLD"
     gene            1208..2503
                     /locus_tag="VFX87_11200"
     CDS             1208..2503
                     /locus_tag="VFX87_11200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4995532.1"
                     /translation="MRVILAVLLLLLLLAGLAQAAGPLAHREVLTNGAVLLVAERPAI
                     PIVVVRVGLPAGAVHDPADALGVANLTAALLTRGTARRTGPELDRAIEFVGGSLEAGA
                     GSDGATVTLSVLKKDLALGLDLLAEVLLQPAFAPEELTRKVSDIQGALRRSEQEPETV
                     AGRELSRMMFPGHPYGRPASGTIESVGKITREQVTAFYQRHYRPDGANIVAVGDVTVA
                     EIRRELLARLGGWSAPSVPVGAVAQASSITAVEERKIVRDLTQTTVLLGRPAIRQDDP
                     DYFPLAVATYVLGGGSASRLYTRVREERGLAYSVYSSLMPARYGASYLVSLQTRTDGV
                     VEAVRLTREEMTRLAREPVAPRELSLAKSYLIGSFPLRLDTSGKLARFLGAVEEIGLG
                     LDYPERYKDRIGRVTAADVQRVAVKYLDPATFSEVQIGR"
     gene            complement(2613..3158)
                     /locus_tag="VFX87_11205"
     CDS             complement(2613..3158)
                     /locus_tag="VFX87_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEX4995533.1"
                     /translation="MRWLWPVLLVILVGGCATAFPESVMSTVDTRITADELLSQPAAV
                     KGARVILGGDILGVQPSPGLTEIELLTRRLRGDDSPERSDRSPGRALLRTPEFLDPAV
                     YAPGRRITVVGEVTGVEERKIGEVPYRYPVITVERIRLWPKDVVLSPAYYPYPWGYSP
                     YGPYYDPFYIGPRRYYPGWWW"
     gene            3253..3807
                     /locus_tag="VFX87_11210"
     CDS             3253..3807
                     /locus_tag="VFX87_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995534.1"
                     /translation="MIAAGLSLICSAPIDAASFTLTLQAREEAVRVGRRSVATENWSG
                     EWRVGGGEGQVVTVMTPFHRLALAARNSAFQDKELKPRDVDSVLKDQQGKVVLWATLR
                     GAKADFARFYTPALVARQQEIKASFTQNERTALRGEDGQYTARCLYVFPAAGLDPRAT
                     VTLLVRDADEKLVAKFTVDLSTMR"
     gene            3831..4241
                     /locus_tag="VFX87_11215"
     CDS             3831..4241
                     /locus_tag="VFX87_11215"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4995535.1"
                     /translation="MTPERFAQGMTFAAYLAQMRTNKERFEKRMADSEITPADREAIR
                     GRKLKILVITEDWCGDALVGFPALARLVAGAPDVEMRVFLRDENPDVMDQYLKRGLYR
                     TIPVFVFFDEQMSELARFMERQDVVTELRTLLKV"
     gene            4255..5223
                     /locus_tag="VFX87_11220"
     CDS             4255..5223
                     /locus_tag="VFX87_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX4995536.1"
                     /translation="MKAIRVHAPGGPESMKLEEVAAPAPAAGQAQVKLEACGVNYIDV
                     YFRTGAYKAPLPLTPGMEGAGTVTAVGPGVSGVKVGDRVAWTGVIGSYAQMLAVPADR
                     LVALPDKLTFKDGAAAMLQGLTAHYLVTSTWPLKKGETALVQAAAGGMGLLLCQMAKM
                     RGATVIGTVSTEEKAALAKAAGADHVILYTKQDFEPEVKRITGGRGVDVVYDGVGAST
                     FDKGLNCLRPRGLMALFGAASGPVPPLDLQRLNAGGSLFITRPSLNHHIASREELMQR
                     STELLGWIRDGKVKLRVEHQFPLEQAAEAHRQLEGRKTTGKILLLP"
     gene            5231..6844
                     /locus_tag="VFX87_11225"
     CDS             5231..6844
                     /locus_tag="VFX87_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX4995537.1"
                     /translation="MLKWSMLKWTLVGLAALVVLVLGALLALPLLLDTPAIQTYASQA
                     ASHALGRPVKFNALSISALPLPTVKLRGLQVAEDPSFGPGPFITVTEGRMRIRIRPLL
                     QGRVELADLTLEEPRIHVVEDAAGRLNVATLGGGALPAPTGSPRVGTSRPGVAAASAV
                     LLSRVRIVKGAIDYQQAGAKSAQFTLDAIDVTVTQATAGEALRVSGSAQAQPGNVKLT
                     IAEATVGVAPGRAFGDAQLKATVDIESRDVAPAAALFVPKPTVSGPLKGRIQVSGTAA
                     RLTASGAVGFDRLTLSAQQPHCLEPKRRSLTLDDVRVPLLYAPPQLESQSVQGKVAKG
                     TVTFRLTVALGPPQVATLRDITVTGVQLEPVLVDYLCQRNAVTGPLVLTGEARLRPQE
                     ALPTLNGAGRLHIGAGRVIGPDLLNALGQALALTDLVSRPLERGRGRPVAGSPLNFDS
                     ITASYTITNGVVRTEDLVYQARDLNLTGVGTYGLVDGRTAMDVIVTQGGNRVKARVAG
                     GPGALTIVPTDVRIKEMKDLKKTLDQLLR"
     gene            complement(6813..7664)
                     /locus_tag="VFX87_11230"
     CDS             complement(6813..7664)
                     /locus_tag="VFX87_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4995538.1"
                     /translation="MRPSRPSVVDFYDRHPINEAQVLESARALRLHAGGPLQPEDLWA
                     WDQDHYGGLGAVETLARRAAIVPGMSVLDVCAGLGGPARFLAHRFCVRVTGVDLTQSR
                     CAAGARLTALVRLSPLVRLLRADAQVLPFRARAFDAAVSQEGLLHVPDKAAVLRECAR
                     VLKPGARLAFSDWVARPRLEENERRRLDEWMAAVTLQSIEGYRELLARAGFGLVEAED
                     LSAEWIGILRERLRMYRGMREQTVARFGQAGYDEYNQLYAFFVGLVEAGKLGGARFSA
                     TADRAFS"
     gene            7762..8904
                     /locus_tag="VFX87_11235"
     CDS             7762..8904
                     /locus_tag="VFX87_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX4995539.1"
                     /translation="MTDAAVNPLKAGLEDVVVSPSEICFIDGHKGRLIYRGYDVDDLV
                     AHSTFEEVTYLLWQGRLPSRKELDTHLKALASTSNRKLPPKLLTMLKLLPKKTTPMEV
                     LRTGVSALSSFDPDAADNSREATLRKSLRLTAQLPTLVAAWERIRRGKAPIAPSPKLN
                     LAGNFLYMMSGKKPTPLAAKTFDTALILHADHEFNASTFAARVTAATLSDLHSASVSG
                     IGALKGPLHGGANEQVMLMVEKIKDPAKADTWIRKALTDKARIMGFGHRVYRVEDPRA
                     KHLKRLALELGQQVGDTSKVQILDTVVRVVGGEKHIFPNVDLYSGAAYAAMGIPTDQF
                     TPIFAMSRIAGWAAHVLEQHGNNRLIRPRSDYSGALDLKYVPLSER"
     gene            8944..9735
                     /locus_tag="VFX87_11240"
     CDS             8944..9735
                     /locus_tag="VFX87_11240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4995540.1"
                     /translation="MTTSVVLVVHNHPALTRACVESLQATRGPFELCVVDNASDEETV
                     EYLDRLARSVPLRYLRNDQNLGLIRALNQGAELATGDLLCFLHNDTELRDPRWLERLQ
                     GAVASTARIGLAGLYGVQRIRRDGRYVGRTILHCLEEAPTLRTPLAEVAVVDGVCLFL
                     ARDVLHAVGGFDEGYGFFHGYDRDLSFAVRELGLRCAVVNAPFVHRGGGTRTGERAPV
                     APPEDLAQRQAALARFTQKWGHRLPCDVRRWPERLRDWLRGLRKV"
     gene            9744..10610
                     /gene="dapF"
                     /locus_tag="VFX87_11245"
     CDS             9744..10610
                     /gene="dapF"
                     /locus_tag="VFX87_11245"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX4995541.1"
                     /translation="MIPFVKGHGLGNDYIVINGADLPTPLTPGQVTRICDRNWGVGSD
                     GILLLVPAWAGGDFGLRILNPDGSEAEKSGNGLRIFAKYLHDHGHAKRDTFTVDTKGG
                     RVECRCHVKDGRMNLVTVEMGRCTFVAPEIPMNGPEREVVKVPLQVGGETLLVTAVSV
                     GNPHCVIFTDRLDEALVRRLGPQVEHHPAFPNRINVQWARVASRSMVDILIWERGAGW
                     TLASGSSSSAVACAAVKNGLCDHGLVTVRMPGGELSVDVRPDWSIRLQGPVEEVYTGT
                     FSRDLMAVLEMS"
     gene            complement(10559..>11786)
                     /locus_tag="VFX87_11250"
     CDS             complement(10559..>11786)
                     /locus_tag="VFX87_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995542.1"
                     /translation="SPPSSALVAIGAERLPIATAPVATATRSIRRAALWTLLLSKLIA
                     GWGVQWDIQWHVLIGRDSFWIPPHVMTYAGVAVAVIVSFGVLAWETLAARPEHVSGAP
                     VLWLLGLTGTRGFHLAAWGIGLTVLAAPIDDLWHRLFGLDVTLWSPPHLLGILGAVIN
                     SLACLVIAREVYPEGSWARLTALVLTGAVLYGGLHLTVDPSTRVAYLHGGVLFYTLAI
                     LSALVLPLAHVPTARLSNSRWAPLTLLAVLIGLGLIGQQIARKGFEVMQPVSVIEQEI
                     AKDPTSPIAVATLMARKEGVPAGQTGGRRHLFALWPALAMALVDARRRPIAATLAYAA
                     ALFALLAWRLPSRPSLAAMAPTLPETLVALLLTLAAGLVAAYAARWLSNALAGGQVLN
                     YDISSTAMRSREKVPV"
BASE COUNT         1771 a         4117 c         4248 g         1650 t
ORIGIN      
        1 cggaggggag agggggcacg tcggataccc tctcccctct ggggagaggg cagggtgagg
       61 ggtagatggg ctcgcggctc ctcggcatca tcgtgaagga gttcatccac ctcaagcgcg
      121 acatgctcgc gctggtgctc gcgctggcgg tgccggtggc catgctgttc atcttcggct
      181 gggccatcaa caccgacgtc aagcacatcc ccaccgcggt cttcgagcag tcggggagcc
      241 tcgaggcccg gctcttcctg gaggcgatgg agaacagcca gtacttcgac gtccgccggc
      301 gggtcagcag ccacaaggag ctgacgcggc tcatcgactc gggcgaggcc aaggtcggcg
      361 tggtgatccc gcccgactac gcccggcggc tcacccggca ggacgccgcg atccaggtga
      421 tcgtggacgc ctcggatccg caggtggcca cctccgcgct caacgcggcc acctcgctcg
      481 gggcccagcg ctcgctccag gtgctgaccc agacgctcga gggcacccgc ctcgcgcggc
      541 agccggagcc gccggtggac gtgcgggtcc gtgcctggta caacccggac ctcgtctccg
      601 ccatcttcat cgtgccgggc ctgatcggcg cgctcctcat gcagaccacc atcaccgcga
      661 tggcggtctc ggtggtccgc gaacgcgaga agggcacgct tgaggcgctg atcgtgagcc
      721 ccatccggcg ctgggagctc ctcctcggca agatcattcc caacctgctg gtggccttcg
      781 gccagatgac gatggccctg ctggtggccc acttcgtctt cgacgtgccg atccggggca
      841 gcctgccgct gctctacgtc ctgtcgctcg tcttcatgat gggcacgctc ggcgtgggca
      901 tcctgctctc gacggcctct cgcacggtgc cgcaggccat gcagctcgcg ttcctcacgt
      961 tcctgccgtc catctatctc tccgggctgc tcttcccgat cgagggcatg cccaccggcg
     1021 cgcagtacct ggccgcgatc atcccgctga cctacttcct gaagatcgtg cgcgggatca
     1081 tcctcaaggg cgtgggcttc gagtacctgt ggccctcgct cgtgccgctg atgatcttcg
     1141 ggacggtcgt gttctccctg gcggtgctca agttccgcaa acagctcgat tgacgagaga
     1201 ggcgctcatg cgagtgatcc tggccgtgct gctgctcctg ctcctgctcg ccggcctcgc
     1261 gcaggcggcg ggtccgctgg cgcaccgcga ggtcctgacg aacggcgcgg tgctgctcgt
     1321 ggccgagcgg cccgcgatcc ccatcgtggt ggtgcgcgtg ggcctgcccg ccggggccgt
     1381 gcacgacccc gccgacgccc tgggggtcgc caacctcacc gccgcgctgc tcacgcgcgg
     1441 caccgcccgg cgcaccgggc cggagctgga tcgcgcgatc gagttcgtgg ggggcagcct
     1501 cgaggcgggc gcggggagcg atggggccac cgtcacgctg tccgtcctca agaaggacct
     1561 cgcgctcggg ctggacctgc tcgccgaggt gctgctgcag cccgcgtttg ctcccgagga
     1621 gctgacgcgc aaggtgtcgg acatccaggg ggcgctgcgg cgatcggagc aggagccgga
     1681 aacggtggcg ggccgcgagc tgagccggat gatgtttccc gggcatccgt acgggcgtcc
     1741 cgcatccggc accatcgaat ccgtcgggaa gatcacgcgc gagcaggtga ccgcgttcta
     1801 tcagcgccac taccggcccg acggggcgaa catcgtcgcg gtgggcgacg tgacggtggc
     1861 ggagatccgc cgcgagctgc tggcgcggct cggcggctgg agcgcgccgt cggtcccggt
     1921 gggcgcggtg gcgcaggcgt cgtccatcac cgccgtcgag gagcggaaga tcgtccggga
     1981 cctgacccag accaccgtgc tcctggggcg cccggctatc aggcaagatg atcctgacta
     2041 ctttccgctg gcggtggcga cgtatgtcct gggcggggga tccgcctcgc gtctgtacac
     2101 gcgcgtgcgg gaggagcggg ggctcgccta ctccgtctac agctcgctca tgcccgcgcg
     2161 ctacggggcc tcgtacctcg tgtcgctgca gacgcggacc gacggggtag tggaggcggt
     2221 gcgcctcacc cgggaagaga tgacgcggct cgcgcgggag ccggtggcgc cgcgggagct
     2281 gtccctcgcc aagtcctacc tgatcggcag cttcccgctg cggctcgaca cctcgggcaa
     2341 gctcgcgcgc ttcctgggag cggtggagga gattgggctc gggctcgact atccggagcg
     2401 ctacaaggac cggatcggcc gggtcactgc ggcggatgtc cagcgcgtcg ccgtgaagta
     2461 cctcgacccc gcgaccttct ccgaggtgca gatcggacgc taagtcatcg gagggagcct
     2521 cgacggcccc ctccgagcct cccccaagaa ccggttgcgc cggcgaagcc ggcgctcgaa
     2581 cagaggttat tcggacgcac tcccggcgcg gctcaccacc accagccggg atagtagcgg
     2641 cggggcccga tgtagaaggg gtcgtagtag ggaccgtacg ggctgtagcc ccaggggtac
     2701 gggtagtagg cggggctcag caccacgtcc ttcggccaga ggcggatgcg ctccaccgtg
     2761 atcaccgggt accgatacgg cacttccccg atcttgcgct cctccacgcc ggtcacctcg
     2821 ccaaccaccg tgatgcgccg gcccggagcg tagacggcgg gatcgagaaa ctcgggcgta
     2881 cgcagcagcg cccggcccgg cgagcggtcg ctgcgctcgg gggagtcgtc gccgcgcagc
     2941 cgccgcgtca ggagctcgat ctcggtgagg cccgggctgg gctggacgcc caggatgtcg
     3001 ccccccagga tcacgcgcgc gcccttgacg gcggccggct ggctcaggag ctcgtcggcg
     3061 gtgatccggg tgtccacggt gctcatcacc gactccggga aagcggtggc gcacccaccg
     3121 accagaatca ccagcaggac cggccagagc cagcgcatga atcgacgata cgccccccgc
     3181 gggggggccg caatcgctgt gctacaatcc gcgcacatga ggcgggctcg ctcatgccgg
     3241 tggtggtgcc tggtgatcgc ggccggcctg tcgctgatct gcagcgctcc catcgacgcc
     3301 gcgtcgttca ccctcacgct gcaggcgcgc gaagaggccg tgcgggtcgg ccggcgcagc
     3361 gtggccacgg agaactggag cggcgagtgg cgcgtgggcg gcggcgaggg ccaggtcgtc
     3421 accgtcatga ccccgtttca ccggctggcc ctggccgcgc gcaactccgc cttccaggac
     3481 aaggagctca agccgcgcga cgtggactcg gtgctgaagg accagcaggg caaggtcgtg
     3541 ctgtgggcga ccctgcgcgg ggccaaggcg gatttcgcgc gcttctacac ccccgcgctg
     3601 gtggcgcggc agcaggagat caaggcctcc ttcacccaga acgagcggac cgcgctccgg
     3661 ggcgaggatg ggcagtacac cgcgcgctgt ctctacgtct ttcccgcggc gggcctggat
     3721 ccgcgggcca ccgtgacgct cctggtccgg gacgccgacg agaagctggt ggcgaagttc
     3781 acggtcgatc tctcgaccat gcgctagagc cggagaggac ctctcccacg gtgaccccgg
     3841 aacggttcgc gcaggggatg accttcgcgg cctacctggc gcagatgcgc accaacaagg
     3901 agcgattcga gaagcgcatg gccgacagcg agatcacccc cgccgatcgg gaggcgatcc
     3961 gcgggcgcaa gctcaagatc ctggtgatca ccgaggactg gtgcggcgac gccctcgtgg
     4021 gctttcccgc cctcgcccgg ctggtggccg gcgcgcccga cgtggagatg cgggtgttcc
     4081 tccgggacga gaacccggac gtgatggacc agtacctcaa gcggggactc taccgcacga
     4141 tccccgtgtt cgtgttcttc gacgagcaga tgagcgagct ggcgcgattc atggagcgcc
     4201 aggacgtggt gaccgagctg cgcaccttgc tgaaggtctg aggaggaccg agccatgaag
     4261 gcgattcgcg tccacgctcc gggtggtccg gagagcatga agctggagga agtggcggcc
     4321 cccgcgccgg cggccggcca ggcccaggtg aagctcgagg cctgcggcgt gaactacatc
     4381 gacgtgtact tccgcaccgg cgcctacaag gccccgctgc cgctcacgcc cggcatggag
     4441 ggcgcgggca ccgtgaccgc ggtcggcccg ggtgtctccg gcgtcaaggt gggcgaccgc
     4501 gtggcgtgga ccggcgtgat cggctcctac gcccagatgc tcgcggtgcc ggcggaccgc
     4561 ctggtggccc tgccggacaa gctcacgttc aaggacggcg ccgccgcgat gctccagggg
     4621 ctcaccgccc actacctcgt cacctcgacg tggccgctca agaagggcga gaccgcgctg
     4681 gtgcaggccg ccgccggcgg catggggctg ctgctctgcc agatggccaa gatgcgcggg
     4741 gcgaccgtga tcgggaccgt gtccacggag gagaaggcgg cgctggcgaa ggcagcgggc
     4801 gcggaccacg tgatcctcta caccaagcag gacttcgagc cggaggtgaa gcgcatcacg
     4861 ggcggccgcg gcgtggacgt ggtctacgac ggcgtcgggg cgagcacctt cgacaagggg
     4921 ctcaactgcc tgcgcccgcg cgggctcatg gccctcttcg gcgcagccag cggcccggtg
     4981 ccgccgctcg acctccagcg cctgaatgcg ggcggctcgc tcttcatcac gcgcccgagc
     5041 ctcaaccatc acatcgcgtc gcgcgaggag ctgatgcagc gctccaccga gctgctcggc
     5101 tggatccgcg acgggaaggt caagctgcgg gtggagcacc agttcccgct ggagcaggcc
     5161 gccgaggccc atcgccagct cgagggccgc aagacgacgg ggaagatcct gctgctgcca
     5221 tgaccgcccg atgctgaagt ggtcgatgct gaagtggacg ctggtcgggc tggcggcgct
     5281 ggtggtgctc gtgctgggcg ccctgctcgc gctgccgctg ctgctcgaca cgcccgcgat
     5341 ccagacctac gcttcacagg ccgcctcgca tgccctgggg cggcctgtga agttcaacgc
     5401 gctctccatc tccgcgctgc cgctgccgac cgtgaagctg cgcgggctgc aggtcgccga
     5461 ggacccgagc ttcgggcccg ggcccttcat cacggtgacc gaaggccgca tgcgcatccg
     5521 catccgcccg ttgctccagg ggcgggtgga gctggccgat ctcacgctcg aagagccccg
     5581 catccacgtg gtggaggacg ccgcgggccg gctcaacgtg gcgacgctgg gcggcggcgc
     5641 gctgcccgcg ccgaccggct cgccgcgcgt gggcacgagc cgtcccgggg tcgccgcggc
     5701 gagcgcggtg ctgctctcgc gcgtgcgcat cgtcaagggc gccatcgact accagcaggc
     5761 gggcgcgaag agcgcccagt tcaccctcga cgccatcgac gtcacggtga cccaggccac
     5821 cgccggcgag gccctgcgcg tcagcgggag cgcccaagct cagcccggca acgtgaagct
     5881 gacgatcgcg gaggccaccg tcggcgtcgc gccggggcgc gcgttcgggg acgcgcagct
     5941 caaggcgacg gtggacatcg agagccgtga cgtcgcgccg gccgccgccc tgttcgtgcc
     6001 caagcccacg gtatccggcc cgctcaaggg ccggatccag gtgtcgggca ccgcggcgcg
     6061 cctgaccgcc agcggcgccg tcggcttcga ccgactcacg ctgtccgccc agcagccgca
     6121 ctgcctggag ccgaagcggc ggtcgctgac gctcgacgac gtccgggtgc cgctgctcta
     6181 cgcgccgccg cagctcgaga gccagtcggt gcaggggaag gtggcaaagg ggacggtgac
     6241 cttccggctg accgtcgcgc tgggcccgcc gcaagtggcc accctgcgcg acatcacggt
     6301 caccggcgtg cagctcgagc cggtgctggt ggactacctc tgccagcgca acgcggtgac
     6361 cgggccgctg gtcctgaccg gcgaggcccg cctgcgcccg caggaggcgc tgcctaccct
     6421 caacggggcc ggccggctcc acatcggggc ggggcgcgtc atcggtcccg atctgctgaa
     6481 tgcgctcggc caggcgctcg ccctgaccga tctggtctcg cgcccgctcg aaagagggcg
     6541 gggccggccg gtcgcgggct cgccgcttaa cttcgactcg atcaccgcga gctacacgat
     6601 caccaacggc gtggtcagga ccgaggacct ggtctaccag gcccgcgacc tcaacctgac
     6661 cggggtgggc acctacgggc tcgtggacgg acggaccgcg atggacgtga tcgtgaccca
     6721 gggcggcaac cgggtgaaag cccgggtggc gggcgggccc ggggcactga cgatcgtgcc
     6781 caccgacgtc cggatcaagg agatgaagga cctcaagaaa acgctcgatc agctgttgcg
     6841 ctgaaccgcg cgccgcccag cttgcccgcc tccacgagcc cgacgaagaa cgcgtagagc
     6901 tggttgtact cgtcataacc cgcctgaccg aagcgggcga cggtctgctc gcgcatcccc
     6961 cggtacatgc gtagccgctc gcgcaggatg ccgatccatt ccgcggagag gtcctccgct
     7021 tcgaccaggc cgaagccggc ccgggccagc agctcgcggt agccctcgat gctctgcagg
     7081 gtgaccgcgg ccatccattc gtcgaggcgg cggcgctcgt tttcctcgag ccggggccgc
     7141 gcgacccagt cgctgaaggc caggcgcgcc ccgggcttga gcacgcgcgc acactcccgg
     7201 aggaccgccg ccttgtccgg cacgtgcagg agcccctcct ggctcaccgc ggcatcgaag
     7261 gcgcgggcgc ggaaggggag gacctgcgcg tcggcccgca ggaggcgcac caggggactc
     7321 aggcggacga gggcggtgag ccgggcgccc gccgcgcatc gcgactgggt caggtccacg
     7381 ccggtgacgc ggacgcagaa gcggtgggcc aggaagcgcg ccgggccgcc caggcccgcg
     7441 cagacgtcga gcacgctcat gcccggcacg atggccgccc gccgggccag cgtctcgacg
     7501 gccccgaggc ccccgtagtg atcctgatcc cacgcccaga ggtcctccgg ctggagcggg
     7561 cccccggcgt gcagccgaag cgcgcgcgcg gactccagca cctgcgcctc gttgatggga
     7621 tgccggtcgt agaagtcgac caccgacgga cgggatgggc gcacgacgcc gagtgtacgg
     7681 ggaggccgcc ttgaagtcac cgggggcgtg tggtagaagg agtcgtttcc attagccccc
     7741 caaggagaac cggagagagc catgacggac gccgcggtca atcccctgaa ggctggtctc
     7801 gaggacgtcg tcgtctctcc gtcggagatc tgtttcatcg acggccacaa gggccggctc
     7861 atctaccggg gctacgacgt ggacgacctg gtggcccact cgaccttcga ggaggtcacc
     7921 tacctcctgt ggcaggggcg gctgccgtcg cgcaaggagc tcgacacgca tctgaaggcg
     7981 ctggcctcga catcgaaccg caagctcccg ccgaagctcc tcacgatgct caagctgctg
     8041 cccaagaaga cgaccccgat ggaggtgctg cggaccgggg tgtcggcgct ctcctcgttc
     8101 gatcccgacg cggccgacaa tagccgggaa gcgacccttc gaaagtcgtt gcgcctcacc
     8161 gcgcagctcc cgaccctggt cgcggcctgg gagcgcatcc gccgcggcaa ggcgccgatc
     8221 gccccgagcc cgaagctgaa cctggcgggc aacttcctgt acatgatgag cggcaagaag
     8281 cccacgccgc tcgccgccaa gaccttcgac accgccctga tcctccacgc cgaccacgag
     8341 ttcaacgcct cgaccttcgc ggcccgcgtg accgccgcca ccctgtccga cctgcattcg
     8401 gcctcggtgt ccggcatcgg cgcgctcaag ggcccgctgc acggcggggc caacgagcag
     8461 gtcatgctga tggtcgagaa gatcaaggac ccggccaagg cggacacgtg gatccggaag
     8521 gcgctcaccg acaaggcgcg catcatgggc ttcggccacc gcgtgtaccg ggtggaggac
     8581 ccgcgggcga agcacctcaa gcggctcgcg ctcgagctcg ggcagcaggt gggcgacacg
     8641 agcaaggtgc agatcctcga cacggtggtg cgcgtggtcg gcggcgagaa gcacatcttc
     8701 ccgaacgtgg atctctactc gggcgcggcc tacgcggcca tggggatccc caccgaccag
     8761 ttcacgccca tcttcgccat gagccgcatc gcgggctggg ccgcgcacgt gctcgagcag
     8821 cacggcaaca accgcctcat ccggccccgc tccgactact cgggcgcgct cgacctgaag
     8881 tacgttccgc tctccgagcg gtaggcggcc gccgggccgg cccccggcgc tgccgtggcg
     8941 gccgtgacga cgagcgtggt gctggtggtg cacaaccacc ccgcgctcac ccgcgcgtgc
     9001 gtggaaagcc tccaggcgac ccgcggccct ttcgagctct gcgtcgtgga caacgcctcg
     9061 gacgaggaga ccgtggagta cctcgaccgg ctcgcgcgct cggtgccgct gcgctacctg
     9121 cggaacgacc agaacctggg gctcatccgg gcgctcaacc agggggcgga gctcgccacc
     9181 ggggacctgc tctgcttcct gcacaacgat acggagctgc gggacccgcg gtggctcgag
     9241 cgcctgcagg gcgcggtggc gtccaccgcg cgcatcggcc tggccggcct ctacggcgtg
     9301 cagcgcatcc gccgtgacgg ccgctacgtg ggccggacca tcctgcactg cctcgaggag
     9361 gcgccgaccc tccgcacccc cctcgcggag gtggccgtgg tggacggcgt ttgtctcttc
     9421 ctcgcgcgcg acgtcctgca cgcggtgggg ggcttcgacg agggctacgg cttcttccac
     9481 ggctacgacc gcgatctctc cttcgcggtg cgggagctgg gcctgcgctg cgcggtggtg
     9541 aacgcgccct tcgtgcaccg gggcgggggc acgcggacgg gcgagcgcgc gccggtggcg
     9601 ccgccggagg acctcgccca gcgccaggcg gcgctggcgc gcttcacgca gaaatggggg
     9661 caccggctgc cctgcgacgt gcggcggtgg cccgagcgcc tgcgcgattg gctccgcggg
     9721 ctccggaagg tatgatgccg cgcatgatcc ccttcgtgaa gggccacggc ctcggcaacg
     9781 actacatcgt gatcaacggc gcggatctgc cgacgcccct caccccaggc caggtgacgc
     9841 gcatctgcga tcgcaactgg ggggtgggct cggacggcat cctgctgctc gtgccggcct
     9901 gggccggtgg ggacttcggc ctccgcatcc tcaacccgga cggcagcgag gccgagaagt
     9961 cgggcaacgg cctgcgcatc ttcgccaagt acctccacga ccacggccac gccaagcgcg
    10021 acaccttcac ggtggacacc aagggcgggc gggtcgagtg ccgctgccac gtgaaggacg
    10081 ggcggatgaa cctggtgacc gtcgagatgg gccgctgcac cttcgtggcg cccgagatcc
    10141 ccatgaacgg ccctgagcgc gaggtggtga aggtcccgct ccaggtgggc ggcgagacct
    10201 tgctcgtcac cgcggtctcg gtgggcaacc cgcactgcgt gatcttcacg gaccgtctcg
    10261 acgaggcgct ggtgcggcgg ctcgggcctc aggtcgagca tcaccccgcg tttccgaacc
    10321 ggatcaacgt gcagtgggcg cgggtggcct cgcgctcgat ggtggacatc ctgatctggg
    10381 agcgcggggc gggctggacg ctggcctcgg ggtcgtcctc gagcgcggtg gcctgcgccg
    10441 cggtcaagaa cgggctctgc gatcacgggc tggtgacggt gcggatgccg ggcggcgagc
    10501 tctccgtgga cgtgcggccc gactggtcca tccggctgca ggggccggtg gaggaggtct
    10561 acaccgggac cttctcgcgg gacctcatgg ccgtgctcga aatgtcgtaa ttcaagacct
    10621 gacccccggc gagcgcgttg gagagccagc gggcggcgta ggcggcgacc aggccggcgg
    10681 ccagggtcag gagtagcgcg acgagcgttt caggcagcgt gggggccatg gcggcgagcg
    10741 aggggcgcga gggcaggcgc caggcgagca gggcgaagag cgcggccgcg taggccagcg
    10801 tggcggcgat ggggcggcgg cgcgcgtcca ccagcgccat cgccagggcg ggccagagcg
    10861 cgaagaggtg gcggcgcccg ccggtctggc cggccgggac gccttccttc cgcgccatga
    10921 gcgtggccac ggcgatcggc gacgtcggat ccttcgcgat ctcctgctcg atcaccgaga
    10981 cgggctgcat gacctcgaag cccttccgcg cgatctgctg gccgatgagg ccgagcccga
    11041 tgagcacggc gagcagggtg aggggagccc agcggctgtt cgagagccgg gcggtcggca
    11101 cgtgggccag cggcagaacc agcgccgaga ggatcgccaa cgtgtagaag agcacgccgc
    11161 catggaggta ggccacgcgc gtggaggggt ccaccgtcag gtgcagcccg ccatagagca
    11221 ccgcgccggt gagcacgagc gcggtaagcc gggcccatga cccctccgga tagacctcgc
    11281 gggcgatcac caggcaggcg agcgagttga tcacggcgcc gaggatgccc agcaggtgcg
    11341 ggggactcca gagggtgacg tcgaggccga agagccgatg ccagaggtcg tcgatcggcg
    11401 cggccaggac ggtcagaccg atgccccacg ccgcgaggtg gaagccccgc gtcccggtga
    11461 ggcccaggag ccacagcacc ggcgcgcccg acacgtgctc agggcgggca gccagcgtct
    11521 cccacgccag cacgccgaag ctcacgatca ccgccacggc cacgcccgca taggtcatca
    11581 cgtggggagg aatccagaag gagtcgcggc cgatcaggac atgccactgg atgtcccatt
    11641 gcacgcccca gcccgcgatc agcttcgaga ggaggagcgt ccagagcgcg gcgcggcgga
    11701 tcgatcgcgt tgcggtggcc actggcgccg ttgcaatggg cagtcgttcg gcgccgatgg
    11761 ccacgagggc gctactggga gggctt
//
LOCUS       DASUFA010000301         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1446414, whole genome shotgun sequence.
ACCESSION   DASUFA010000301 DASUFA010000000
VERSION     DASUFA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446414"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFX87_11255"
     CDS             complement(<1..708)
                     /locus_tag="VFX87_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4995543.1"
                     /translation="MISALIVGGGSIGKRHLRNLLATGRSAAIVDPREDRRVEIANKH
                     PEAKVYATLDDALAQGPYLSGFICLPTAYHLEPAMRLAQRDMHIFMEKPVSHSLDGIP
                     ALLDVIERRKLVGMTGFCLRFFKPLQKAKELIEAGRVGRIVTARSFTGAYLPDWHPWE
                     DYRSFYMAKKAQGGGVLLDECHAFDWMQWLCGPITGVCSVVGTFSDLEVETDDVCEVI
                     ARFGPRAVGSIHLDMVDR"
     gene            complement(705..1718)
                     /gene="neuB"
                     /locus_tag="VFX87_11260"
     CDS             complement(705..1718)
                     /gene="neuB"
                     /locus_tag="VFX87_11260"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEX4995544.1"
                     /translation="MNKTFLIAEAGVNHNGDMALARALVDAAAAARADAVKFQTFVVD
                     RLVTREAATAEYQRRALGSEQSQYEMLARLELSAADHEMLAAHCAARGIEFMSTPFDP
                     ESARFLKRLGVRRIKISSGDVTNLPMLTVVGALGLPVILSTGMADLDEVAAAVATLRS
                     AGVPDLALLQCVSNYPADPALTNLRVMDTLARAFGTPVGLSDHSVGIAVAIAAAARGA
                     AYIEKHFTLDRSLPGPDHQASLLPEELRALVSAVRQVESALGDGVKRPAPSELAVRAV
                     ARKSLVTARDLPAGATLAAEDVVILRPGTGLSPAALPRVLGRRTARAISHHTPITEDM
                     LLP"
     gene            1763..2383
                     /locus_tag="VFX87_11265"
     CDS             1763..2383
                     /locus_tag="VFX87_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX4995545.1"
                     /translation="MQRQRERNLEILTAIGEGAPVTQRALAEQLGVALGLANLCLKRL
                     AGKGFIKIVEFPKKPAARKRLRYVLTPKGMAEKSRLSYEYMAYSLQLFRRTRGHLRET
                     MARFHQGGMKRFALCGVGEAAELAYLTLREFGLEPVGVFDGAAGGLFLGFPVRALAEL
                     SADEVDGVIVATFDRPEQRVAQLTRLGVPADRCLTLRRLTTPGTRR"
     gene            2380..3054
                     /locus_tag="VFX87_11270"
     CDS             2380..3054
                     /locus_tag="VFX87_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEX4995546.1"
                     /translation="MSAPTARSRGSARRLLILGAGGHGRAVADLARACGFTVAGFTDR
                     SLSAGSPRTPVLGTDAELAALIRPQQIDGGVVGVGNTALARRGQLYGVLVAAGVPQPA
                     LVHPRAVASPSSEVGAGSVVFAGSVLGSEVRIGVNAVLYSGVVVEHECVIGDHAYLSP
                     GVVLSGQVTVEAGAFLGAGAVVLPGLRIGAGAVVAAGAVVTADVPDGATVMGVPARAA
                     RSGESG"
     gene            complement(3008..>3691)
                     /locus_tag="VFX87_11275"
     CDS             complement(3008..>3691)
                     /locus_tag="VFX87_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995547.1"
                     /translation="DFFFGGFDKLLFITVPLAFGFWAFADLGIALLFGEAFRPAAVPL
                     RILILAALVSNIVNPYTFILYALDEAGRFVPVNVLRVIVYVAALGALVLPGDVTLGLG
                     GLWPGAPGAAAARLVLVLFPCWIYFQWSRNLAAIPFYPATWAYLGGFGLMLATFHALL
                     AGAAASLGAGVWWVAIPAAMVSVGLYAVYLFVVHPGTRENLRYTLALFSPGDFIRFLR
                     SGLRGPARP"
BASE COUNT          555 a         1268 c         1363 g          505 t
ORIGIN      
        1 gcgatcgacc atgtcgagat ggatggagcc gaccgcgcgg gggccgaacc gcgcgatcac
       61 ctcgcacacg tcgtcggtct cgacctcgag gtcgctgaag gtgccgacca ccgagcagac
      121 gcccgtgatc ggcccgcaga gccactgcat ccagtcgaag gcgtggcact cgtcgagcag
      181 cacgccgccg ccctgggcct tcttggccat gtagaacgag cggtagtcct cccaggggtg
      241 ccagtccggc aggtaggcgc cggtgaagct gcgcgcggtc acgatgcggc cgacgcggcc
      301 cgcctcgatc agctccttgg ccttctgcag cggcttgaag aagcgcaggc agaagccggt
      361 catgcccacg agcttccgcc gctcgatcac gtcgagcagg gcaggaatgc cgtcgagcga
      421 atgcgagacc ggcttctcca tgaagatgtg catgtcccgc tgcgccagcc tcatcgccgg
      481 ctccaggtgg taggcggtgg gcaggcagat gaagccgctc aggtagggac cctgcgcgag
      541 cgcgtcgtcg agggtcgcgt agaccttggc ctccgggtgc ttgttcgcga tctcgacccg
      601 ccggtcctcg cgggggtcga cgatcgccgc ggaccgcccg gtcgcgagga ggttgcgcaa
      661 gtggcgcttg ccgatggagc cgccgccgac gatgagcgca gagatcatgg taggagcatg
      721 tcctcggtga tgggagtgtg gtgagagatg gcgcgcgcgg tccgccggcc cagcacccgc
      781 gggagcgcag cgggcgagag cccggtgccc ggacgcagga tgaccacgtc ttcggcggcg
      841 agcgttgcgc cggccggcag gtcgcgcgcg gtcaccaggc tcttgcgcgc caccgcccgg
      901 accgccagct cgctcggcgc cggccgcttg acgccgtcgc ccagcgcgga ctccacctgg
      961 cgcaccgccg acaccagcgc gcgcagctcc tcggggagga gcgaggcctg gtggtccggg
     1021 ccgggtagcg agcgatccag cgtgaagtgc ttctcgatgt aggccgcgcc gcgtgcagcc
     1081 gccgcgatgg ccaccgcgat cccgacggaa tggtcggaca ggccgaccgg agtgccgaag
     1141 gcccgcgcga gcgtgtccat gactcgcagg ttggtgaggg cggggtccgc cgggtagttg
     1201 ctgacgcact ggagcagcgc caggtcgggg actcccgccg agcgcagcgt ggcgaccgcg
     1261 gccgccacct cgtcgagatc cgccatgccg gtggacagga tcaccgggag gccgagcgca
     1321 cccaccacgg tgagcatcgg caggttggtc acgtcgcccg aggagatctt gatccgccgc
     1381 acgcccaggc gcttcaagaa gcgggcgctc tccgggtcga acggggtgga catgaactcg
     1441 atcccgcggg ccgcgcagtg ggcggccagc atctcgtggt cggccgccga cagctcgagc
     1501 cgggcgagca tctcgtactg gctctgctcc gagccgagcg cgcgccgctg gtattcggcg
     1561 gtggccgcct cgcgcgtgac gagccgatcc accacgaagg tctggaactt gaccgcgtcg
     1621 gcgcgcgccg cggccgccgc atcgacgagg gcgcgggcga gcgccatgtc accgttgtgg
     1681 ttcacgcccg cttccgcgat gaggaaggtc ttattcactt gacgcatctt acaccagtat
     1741 gttcaacttg tgaacgtcgt atatgcagcg ccagcgcgag cgaaatctgg agatcctgac
     1801 cgccatcggc gagggcgcgc cggtcaccca gcgcgccctc gccgagcagc ttggggtcgc
     1861 cctggggctg gccaatctct gcctcaagcg cctcgccggc aagggcttca tcaagatcgt
     1921 ggagttcccc aagaagccgg cggcccgcaa gcgcctgcgc tacgtcctga ccccgaaggg
     1981 catggccgag aagagccggc tctcctacga gtacatggcc tactcgctgc agctcttccg
     2041 ccgcacgcgc ggccatctgc gggagaccat ggcccgcttc caccagggcg gcatgaagcg
     2101 cttcgcgctc tgcggggtgg gggaggcggc cgagctcgcg tacctcacgc tgcgcgagtt
     2161 cgggttggag cccgtcggcg tcttcgatgg cgcggcgggc ggcctgttcc tcggcttccc
     2221 ggtgagggcc ctcgcggagc tcagcgccga cgaggtggac ggcgtgatcg tggccacctt
     2281 cgaccggccg gagcagcggg tggcccagct gacccgtctc ggtgtgccgg cggatcgctg
     2341 cctgaccctg cggcgcctca ccacgcccgg gacccgccga tgagcgcgcc caccgcccgc
     2401 tcacgcggct cggcgcgccg cctgctgatc ctgggagcgg ggggccacgg ccgcgcggtg
     2461 gccgatctcg cccgggcctg cggtttcacg gtcgccggct tcaccgaccg ctccttgtcc
     2521 gcgggtagcc cccgcacccc ggtgctcggc accgacgcgg agctcgccgc tctcatccgc
     2581 ccacagcaga ttgacggcgg cgtggtcggc gtcggcaata cggccctggc gcgccgcggc
     2641 cagctctacg gcgtgctggt ggccgccggg gtcccccagc cggccctggt acatccgcgc
     2701 gcggtcgcgt cgccctcgtc cgaggtcggc gcgggatcgg tggtgttcgc gggcagcgtc
     2761 ctcggtagcg aggtgcggat cggggtgaac gcggtcctct acagcggggt cgtggtggag
     2821 cacgaatgcg tgatcggtga tcacgcctat ctctcgcccg gcgtggtgct ctcgggccag
     2881 gtgacggtgg aggccggcgc gttcctcggc gcgggcgcgg tggtgctgcc gggcctgcgc
     2941 atcggcgccg gtgcggtggt ggccgccggc gcggtggtga ccgcggacgt gccggatggc
     3001 gcgaccgtca tgggcgtgcc ggcccgcgca gcccgctccg gagaaagcgg atgaagtctc
     3061 ccggcgagaa gagcgcaagc gtatagcgaa ggttctcccg cgtccccggg tgcaccacga
     3121 acaggtagac cgcgtagagc cccaccgaca ccatggcggc cgggatcgcg acccaccaca
     3181 cgcccgctcc gagtgacgcg gcggcgcccg ccaggagggc gtggaaggtc gcgagcatca
     3241 gcccgaagcc gccgaggtag gcccaggtag ccgggtagaa cgggatcgcg gcgagattcc
     3301 ggctccactg gaaatagatc cagcagggga agagcacgag gacgagccgg gcggccgcgg
     3361 cgccgggcgc tcccggccag agcccgccga ggcccagggt cacgtcaccg ggaagaacca
     3421 gcgcgccgag cgccgcgacg tagacgatca cgcgcagcac gttgaccggc acgaagcgcc
     3481 cggcctcgtc cagggcgtag agaatgaacg tgtacgggtt gacgatgttc gagacgagcg
     3541 cggccaggat caggatgcgc aggggcaccg cggccggacg gaacgcctcg ccgaacagca
     3601 gtgcgatgcc caggtccgcg aaggcccaga agccgaaggc gagagggacc gtgatgaaga
     3661 gcagcttgtc gaacccgccg aagaagaagt c
//
LOCUS       DASUFA010000302         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_401545, whole genome shotgun sequence.
ACCESSION   DASUFA010000302 DASUFA010000000
VERSION     DASUFA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401545"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2575)
                     /locus_tag="VFX87_11280"
     CDS             complement(<1..>2575)
                     /locus_tag="VFX87_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX4995548.1"
                     /translation="VRITADHKVLTVGRGDVAVADLTAGNRLMLRGPGFGPRSLATDL
                     ALGVGVAAAEGYLTQSVVHGRAQESLVLTTDAGESPVLAAVADAVHAGRAALQAVGGL
                     GRQAGSGVITRSAATARLMLSDAAAVAVFREFAILDEGAAAIRFAPAIFELDRPALAA
                     VLRGLFTADATVANYGEQSRYISLDSTSLELLRQTQLLLLAFGIKARLYEDTRLAEAT
                     AARPHPTHSLRISRASCPLFEQAIGFHAESPKAQALARLNGDEPGTGEELTDEVLAIE
                     PAGEADVFDLTEAVTHHFVAGGLVVHNCSEYLFLDDTACNLASINLVKFLREDGSFDV
                     DGFRHACRLWTSVLEISVLMAAYPSPAIAQRSWDFRTLGLGYANMGTVLMRRGIPYDS
                     PEAIAICGAMTAILCGEAYATSAEMARDLGPFPGYHKNQAHMLRVMRNHRRAAYGVAP
                     VEYEGLSITPVGIDPAHCPVPLLQAARETWDRAVGLGEQFGYRNAQVTVLAPTGTIGL
                     VMDCDTTGIEPDFSLVKFKKLAGGGYFKIINQSLPLALETLGYTPGEIDDIVAYCVGR
                     KTLRGAPFINHDTLRAKGFDDAGLQRLEASIETAFDITFAFNAWTLGEGYVASRLGIN
                     EAQLAEWNGNLLRALGFSAAEIEAANDYCCGTMTVEGAPLLKPAHLPVFDCANRCGKK
                     GTRFIATEAHIRMMAAAQPFISGAISKTINMPQDATIEDVKWAYDLSWRGMLKAVALY
                     RDGSKLSQPLNSTTDDEEEQAAAADVEAVAEKMTERVVTQYLRERHRLPDRRSGYTQK
                     AVVGGHKVYLRTGEYQDGTVGEIFLDMHKEGAAFRSLMNCFAIAISLGLQHGVPLEEF
                     VD"
BASE COUNT          357 a          856 c          970 g          392 t
ORIGIN      
        1 gtccacgaac tcctcgagcg gcacgccgtg ctgcaggccg agcgagatgg cgatggcgaa
       61 gcagttcatc agggagcgga aggccgcccc ttccttgtgc atgtcgagga agatctcgcc
      121 gaccgtcccg tcctggtact cgccggtgcg caggtagacc ttgtggccgc cgaccaccgc
      181 cttctgcgtg tagcccgagc ggcggtcggg cagccgatgc cgctcgcgca ggtactgggt
      241 gaccacccgc tcggtcatct tctcggccac cgcctccacg tccgcggccg ccgcctgctc
      301 ctcctcgtcg tcggtcgtgg agttgagcgg ctgtgagagc ttggagccgt cgcggtagag
      361 ggccaccgcc ttgagcatgc cccgccacga gaggtcgtag gcccacttga cgtcctcgat
      421 ggtggcgtcc tgcggcatgt tgatggtctt gctgatggcg cccgagatga acggctgggc
      481 cgccgccatc atccggatgt gcgcctcggt ggcgatgaag cgcgtgccct tcttgccgca
      541 gcggttggcg cagtcgaaga ccggcaggtg ggcgggcttc agcagcgggg cgccctcgac
      601 tgtcatggtg ccgcagcagt agtcgttggc cgcctcgatc tcggcggcgc tgaagcccag
      661 cgcgcggagc aggttgccgt tccactcggc gagctgcgcc tcgttgatcc ccagccgcga
      721 ggcgacgtag ccctcgccga gcgtccaggc gttgaaggcg aaggtgatgt cgaacgcggt
      781 ctcgatcgag gcctccagcc gctgcaggcc cgcgtcgtcg aagcccttgg cccggagcgt
      841 gtcgtggttg atgaacgggg cgccccgcag cgtcttgcgc ccgacgcagt aggccacgat
      901 gtcgtcgatc tcgcccggag tgtagccgag cgtctcgagc gccagcggca ggctctggtt
      961 gatgatcttg aagtagccgc cgcccgccag cttcttgaac ttcaccagcg agaagtcggg
     1021 ctcgatgccg gtggtgtcgc aatccatgac gaggccgatg gtgccggtcg gcgccaggac
     1081 cgtgacctgg gcgttgcggt agccgaactg ctcgccgagg ccgaccgccc ggtcccacgt
     1141 ctcgcgcgcg gcctggagca gcgggaccgg gcagtgggcg ggatcgatac cgaccggcgt
     1201 gatgctgagg ccctcgtact caaccggggc gacgccgtag gcggcccgcc ggtggttgcg
     1261 catcacgcgc agcatgtggg cctggttctt gtggtagccg gggaaggggc cgaggtcgcg
     1321 cgccatctcc gccgaggtcg cgtaggcttc cccgcacagg atcgcggtca tcgcgccgca
     1381 gatggcgatg gcctccgggg aatcgtaggg gatgccgcga cgcatgagca cggtgcccat
     1441 gttggcgtag cccaggccca gggtgcggaa gtcccagctc cgctgggcga tcgccgggct
     1501 cgggtaggcg gccatcagga cgctgatctc gaggaccgag gtccacaggc ggcaggcgtg
     1561 gcggaagccg tccacgtcga agctgccgtc ctcgcggagg aacttcacca ggttgatgga
     1621 cgccaggttg cacgcggtgt cgtcgaggaa gaggtactcc gagcagttgt gcacgacgag
     1681 cccgcccgcg acgaagtggt gcgtgaccgc ctcggtcagg tcgaagacgt ccgcctcccc
     1741 cgccggctcg atggcgagca cctcgtcggt cagctcctcg ccggtccccg gctcgtctcc
     1801 gttgagccgg gcgagggcct gcgccttcgg gctctccgcg tggaagccga tcgcctgctc
     1861 gaagagcggg caggaggcgc ggctgattcg cagcgagtgg gtcgggtggg gccgcgccgc
     1921 cgtcgcctcg gccagccgcg tgtcctcgta gagccgcgcc ttgatgccga aggcgagcag
     1981 gaggagctgg gtctggcgca gcagttcgag ggacgtcgag tcgagggaaa tgtagcgcga
     2041 ctgctcgccg tagttggcca cggtggcatc cgcggtgaag aggccgcgga gcaccgcggc
     2101 cagggccgga cggtccagct cgaagatggc gggggcgaag cggatggcgg cggcgccctc
     2161 gtcgaggatc gcgaactcgc ggaagaccgc caccgcggcg gcgtcgctca gcatgaggcg
     2221 ggcggtggcg gcgctccggg tgatgacgcc gctgccggcc tgccggccga gcccgcccac
     2281 cgcctggagc gcggcgcggc cggcgtgaac cgcgtcggcg accgcggcca gcaccggaga
     2341 ttcgccggca tcggtggtga ggacgaggga ttcctgcgcg cggccgtgga cgaccgactg
     2401 cgtcaggtag ccttccgccg ccgccacgcc gacgcccagc gcgaggtcgg tggcgaggct
     2461 gcgcgggccg aagccggggc cccggagcat caggcggttg cccgcggtga ggtcggccac
     2521 cgccacgtcg ccgcggccca ccgtgaggac cttgtgatcg gcggtgatgc gcacc
//
LOCUS       DASUFA010000303        10234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1112624, whole genome shotgun sequence.
ACCESSION   DASUFA010000303 DASUFA010000000
VERSION     DASUFA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112624"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..366)
                     /locus_tag="VFX87_11285"
     CDS             complement(34..366)
                     /locus_tag="VFX87_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX4995549.1"
                     /translation="MSLSWDEFARVDMRVGVVLAAHEFPEARRPALKLEIDFGPLGVM
                     RSSAQITDHYTPAALVGRRVVAVVNFPPKRIGPFTSEVLVLGAYNEAGEVILLAPDLD
                     VEPGARIG"
     gene            complement(381..1529)
                     /locus_tag="VFX87_11290"
     CDS             complement(381..1529)
                     /locus_tag="VFX87_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX4995550.1"
                     /translation="MRPALALRACALVALALLVCAGPAAASGDIRVAIAEGLRVVEVG
                     GGPMMVSDLAGRAVVEDSPTWLRATWKVGALEVSGGGRRVDGLRLAPGDTGGLRFNGR
                     EYPGALEILKTGDTLTVINELPLEEYLAGAVKAEAGDKMPVEMLKAQAVVARTYAAYH
                     RRLNAEKAFHLVASTLNQQYIGRVPTDSPVWTAVKETAGQVLLWNGELFPAFYHTDSG
                     GYTEDPRAVFATGNMPALKPVRVEFPSASPHHRWRLDLPLADLSAALLRGGVSVGRVV
                     ALDVLERSTSLRVARIAVRGTAGTVTLRGNDLRRLVGYDTLKSTLFAVAVAGPVARFV
                     GRGYGHGVGLDQWSAKTMADQGYKAPQIIAYYYPGAALATLPAASAAR"
     gene            complement(1529..1738)
                     /locus_tag="VFX87_11295"
     CDS             complement(1529..1738)
                     /locus_tag="VFX87_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX4995551.1"
                     /translation="MLLLFGLLMVLLGLVLLVAGHFSGKVPWLGRLPGDIHIQRGSWT
                     FYFPLATCLIVSAVLTLLFSLFSRR"
     gene            complement(1753..2787)
                     /gene="ruvB"
                     /locus_tag="VFX87_11300"
     CDS             complement(1753..2787)
                     /gene="ruvB"
                     /locus_tag="VFX87_11300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX4995552.1"
                     /translation="MTDEARVMSRVALPEETQVERQLRPQRLAEYVGQRAAVESLRVS
                     VEAARQRGEPLDHVLLSGPPGLGKTTLATIMANEMQANIVTTAGPSLERGADLMGILT
                     NLAEKDVLFIDEIHRLPRVVEELLYPAMEDFSVNFVMDKGLNARTIRIPLRPFTMVGA
                     TTRPGMLSSPLRERFGIFHHLDFYSGEDLTAIINRSAAILGAPIAPGGAEAIARRARG
                     TPRIANRLLRRVRDYAQVKADGTVTAEVAGRALDQEGVDEAGLDRLDRRFLTAIIEHY
                     KGGPVGLEAIAATINDEAETLAEVVEPFLLKIGYVVRSPTGRRATPAAYAHLGYALPA
                     SPGGQGQLPL"
     gene            complement(2801..3454)
                     /gene="ruvA"
                     /locus_tag="VFX87_11305"
     CDS             complement(2801..3454)
                     /gene="ruvA"
                     /locus_tag="VFX87_11305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX4995553.1"
                     /translation="MIASLRGKLRRKLEDRVVVEAAGVGYEVFVPPVTQQELLHAKAA
                     DGDAADEVSLEIHYHATQNQPRPVLIGFTSELDREFFEKLITVKDVGPMVAARSLAAP
                     VGEIAAAIARQDEGYLRRLPGIGPQKAKNIVAQLQAKVAKFALAKGEAASPEPVAAAA
                     APDEEALRGMVFDILVKQLGYRPSEAAPIIGAALARRPGVTTPEELLDEIYRGGRKG"
     gene            complement(3451..4017)
                     /locus_tag="VFX87_11310"
     CDS             complement(3451..4017)
                     /locus_tag="VFX87_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX4995554.1"
                     /translation="MGVDPGLVATGFGVLEVGPGGVSVCDAGVISTTTGQPLEARLNA
                     LHRAVHRIIELQSPGLLVVEDLYTEYKFPRTAILMGHARGVIYLAARQLGVGVLALAP
                     SEVKRAMTGNGAAGKGQMQRAVQTVLGLKELPRPSHVADALGLAATGMARITGKVAAK
                     WPPHSPNGGQPTSEAVRRFISSRVTGRP"
     gene            complement(4031..4783)
                     /locus_tag="VFX87_11315"
     CDS             complement(4031..4783)
                     /locus_tag="VFX87_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4995555.1"
                     /translation="MSGHSRWSQIKRKKGKTDVQRGKLFSKILREITVAAKNGGGDPK
                     GNLRLKAATESARAANMPADNIKRAIQKGTGELPGESYEEISYEGYGAGGVAVMVRTL
                     TDNKNRTGPEIRHIFEKNEGRMGTSGCVAWMFDRRGMIEVDAQKVKEDDLLEKALDSG
                     ATDVKQVEKVFEVTASPDEMEAVRQALERQGMPVLEARVDMVPQSTVKVEGKEAAAVL
                     RLIEALEEQDDVQSVYSNYDIADEVIDAITAA"
     gene            complement(4786..5868)
                     /locus_tag="VFX87_11320"
     CDS             complement(4786..5868)
                     /locus_tag="VFX87_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4995556.1"
                     /translation="MLSISDTATEMVNWVFPEHAGAPGQIHGGRMMQWIATCGTIAAS
                     RVARGNVVLGAMDDIDFLHPVKVGEVAVLRAQVEYVGRCSLEVGVRVYAEKPATGERA
                     LTLSSHLVFVKVDEHVKPVPVPQAVVPRGSDEEAMHAAAGERRARRLARFARRAERMR
                     EVEDDAPEQLRWRFESCRSVLPEDLLFGNTMFAGKLLLDIDEAGGILSMRYCRGLVMT
                     ACLDALDFYAPVQSNEVVTFQAGLNHVGRSSLEIGVKVLSEVPWTGEIKHACTAYLTF
                     VHLGSDPSAPLRPQPCPPFTPETPAEQRRWVGALERRARRLERVQVVRGAAAGEPSEY
                     KKAVIEVRGAAAGEPSEYKKAVTEGR"
     gene            6044..7252
                     /locus_tag="VFX87_11325"
     CDS             6044..7252
                     /locus_tag="VFX87_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4995557.1"
                     /translation="MEFEYSKKTKMYMEQLTDFMNKHVVPNEQVFADQLNSGPTRWQI
                     PPIMEELKAKARERGLWNLFLPESERGAGLTNLEYAPLCEVMGRSPIAPEVFNCSAPD
                     TGNMEVLERYGTPEDKKQWLQPLLEGKIRSAFAMTEPKVASSDATNIESRIERQGDHY
                     VINGHKWWTSGIGDPRCKVLIFMGKTDPKNPDKYKQQSMILVPRDTPGIKVVRMLTVF
                     GYDDAPHGHGEVLFENVRVPAANILLGEGRGFEIAQGRLGPGRIHHCMRQIGVAERAI
                     ELMCKRATRRIAFGKPLADNDITLERIAESRIEIDQARLLVLHAAYMMDTVGNKAARQ
                     AIAEIKVAVPSITGRVVERAMQLHGGGGVSQEFPLASMWAHSRTLRFADGPDEVHRRQ
                     IGRLELRKHS"
     gene            complement(7386..8945)
                     /locus_tag="VFX87_11330"
     CDS             complement(7386..8945)
                     /locus_tag="VFX87_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995558.1"
                     /translation="MWSACRRIAITLAFVLTLAGEAWAQGGVLRVGLPSVPAPLDPAT
                     ALEGPVSVIARQIFDTLVQYRDVTSDIEPGLATQWSVSRDGLVWTFRLRDGVRFHDGT
                     VLTAQHVVESLDRVIQPTAPHAPSPNLAAPRLIRGTPGVVKEVKAADARTVQISLLLP
                     YAPLPAVLAHPAFSIVLASSAGQRWVGTGPFALTEASLGRVVLDANPGYWGGPPRSTR
                     LVFLDAGEDGRGEAMLEAQAVDVLLTSVAPSRPAGTQSIPSWRIGYLALDAEREPFSR
                     LKARRAVAAALDPGVLAPAVAPTGVPLLSFLPLGVWGRRDGSPVLEANPVNAKRLLTE
                     AGFPQGTSAGLLISDGDRRPDQARVAEAIRASLATAGISVPIQVEAPEAALQLARTGQ
                     HQMALLEARVEAGDPHFLLYPLTSSEGATKGHAALNMSFYRNRTLDDLLIRASQVSFR
                     PERQRLYVRAQAMLAEEMPWVPLYVRLHWAATRTEVRNLRLHPSGNPRFDRVWIDAPP
                     AAAAPVPSAQR"
     gene            9377..>10234
                     /locus_tag="VFX87_11335"
     CDS             9377..>10234
                     /locus_tag="VFX87_11335"
                     /inference="COORDINATES: protein motif:HMM:NF036798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX4995559.1"
                     /translation="MKEFDKKGNPSREPASEGMKGLSKFLPMTDSNYRRRLTEYEVQV
                     QDLQAYVRSLETETGRLRKKLEDAPKEFMILENKLREANRQLVQAFNQNEKLVNALYE
                     AREQITSLKEEVDKLCAPPSTYGVYLSVNEDGTVNILSQGRKVKVNLHPSIKAEDIKP
                     GQELILNEGLNVVETAGYEIQGEVVILKELLDAERAIVTQRADEDRVGIIAEPLRSAK
                     LKIGDHLLIDAKSGYLLEKLPKSEVEDLTLEEVPDIDYEDIGGLTSQIETIKDAVELP
                     YLYADYYREH"
BASE COUNT         1602 a         3640 c         3531 g         1461 t
ORIGIN      
        1 ccgccgcccc gcctcacgcg acgacgcgcg gagctacccg atccgcgccc caggctccac
       61 gtcgagatcg ggcgcaagga ggatgacctc gccggcctcg ttgtacgcgc ccagcaccag
      121 cacctcggag gtgaacgggc caatccgctt cggcgggaag ttgacgaccg ccaccacgcg
      181 ccggcccacg agcgcggccg gcgtgtagtg atcggtgatc tgcgcgctcg accgcatgac
      241 gccgagcggg ccgaagtcga tctcgagctt gagcgcgggc cgccgcgcct ccgggaactc
      301 gtgagccgcc agcaccacgc ccacgcgcat gtccacgcgc gcgaactcgt cccaggacag
      361 gctcacgccg gtcggagcgc tcagcgggcc gcggacgcgg ccggcagcgt ggcgagcgcg
      421 gcccccgggt agtagtaggc gatgatctgc ggcgccttgt agccctggtc ggccatggtc
      481 ttggcgctcc actggtcgag cccgaccccg tggccgtagc cgcggccgac gaagcgggcc
      541 accggacccg ccaccgccac cgcgaagagc gtgctcttga gcgtgtcgta gccgaccagg
      601 cggcgcaggt cgttgccccg gagcgtcacg gtgcccgcgg tgccgcggac cgcgatccgc
      661 gccacccgca gggaggtgct gcgctcgagg acgtcgagcg ccaccacgcg ccccaccgag
      721 acccctcccc gcaggagtgc ggccgagaga tcggcgagag gcagatcgag gcgccagcgg
      781 tgatgcggcg aggcggaggg aaactcgacg cgcaccggtt tcagcgcggg catgttcccc
      841 gtcgcgaaga ccgcgcgcgg atcctcggtg tacccgccgc tatcggtgtg gtagaaggcg
      901 gggaacagct cgccgttcca cagcagcacc tggccggccg tctccttcac cgccgtccag
      961 accggcgaat ccgtcggcac ccggccgatg tactgctgat tcagcgtcga ggcgacgaga
     1021 tggaacgcct tctcggcgtt gagccgccgg tgataggccg cgtaggtacg cgccacgacg
     1081 gcctgcgcct tcagcatctc gaccggcatc ttgtcgcccg cctcggcctt caccgccccc
     1141 gccaggtatt cctcgagcgg cagctcgttg atcacggtga gcgtgtcgcc ggtcttcagg
     1201 atctcgagcg cgccggggta ctcgcggccg ttgaagcgga gacccccggt gtcgccgggg
     1261 gccagccgca ggccgtccac gcggcgcccg ccgccgctga cctcgagcgc gccgaccttc
     1321 cacgtggcgc ggagccaggt cggcgaatcc tccaccaccg cccggccggc gagatcgctc
     1381 accatcatcg gcccaccgcc cacctcgacg acacgaagcc cctcggcgat ggccacgcgg
     1441 atgtccccgg aggccgccgc cgggcccgcg cagacgagca gggccagcgc gacgagcgcg
     1501 cacgcccgga gagcgagcgc cgggcgcatc agcgccggct gaaaagcgag aagagcagcg
     1561 tcaggaccgc ggagacgatg agacaggtgg cgagcgggaa gtagaacgtc cagctcccgc
     1621 gctggatgtg aatgtctccc ggcaagcgtc cgagccatgg caccttcccc gagaagtgcc
     1681 ccgcgacgag caggaccagt cccaggagta ccatcaggag cccgaatagc agaagcattt
     1741 tgcccagcgg aatcacagcg gtagctgccc ctgccctccc ggagacgccg gcagcgcgta
     1801 gcccaggtgc gcgtacgccg cgggcgtcgc gcgccggccg gtcggactgc gaaccacgta
     1861 gccgatcttc agcaggaagg gctccaccac ttccgcgagg gtctcggcct cgtcgttgat
     1921 ggtggcggcg atcgcttcca gacccacggg cccgcctttg tagtgctcga tgattgcggt
     1981 gagaaagcgc cggtcgaggc gatcgagccc ggcctcgtcc acgccctcct ggtcgagcgc
     2041 gcggccggcc acctccgcgg tgacggtgcc gtcggccttc acctgcgcgt agtcgcgcac
     2101 ccgccgcagc aggcggttgg cgatgcgcgg cgtcccccgc gcgcgccggg cgatcgcctc
     2161 cgcgccgccg ggtgcgatgg gcgctccgag gatggccgcg gagcggttga tgatcgcggt
     2221 caggtcctcg cccgagtaga aatccaggtg gtggaagatc ccgaagcgct cgcgcagcgg
     2281 cgaggagagc atgcccggtc gcgtggtcgc gcccaccatg gtgaagggcc ggagcggaat
     2341 ccggatggtg cgggcgttca agcccttgtc catcacgaag ttgaccgaga agtcttccat
     2401 ggccgggtag agcagctcct cgaccacgcg cggcagccgg tggatctcgt cgatgaagag
     2461 cacgtccttc tcggcgaggt tggtcaggat gcccatcagg tccgcgccgc gctcgaggga
     2521 cgggccggcg gtggtcacga tattggcctg catctcgttg gccatgatgg tggccagcgt
     2581 ggtcttgccg agtcccggag ggcccgagag cagcacgtga tcgagcggct ccccgcgctg
     2641 ccgcgccgcc tcgaccgaca cgcgcaggct ctcgaccgcc gcgcgctgcc ccacgtactc
     2701 ggcgaggcgc tgcggccgga gctgccgctc gacctgggtc tcctccggca gggcgacgcg
     2761 gctcatcacg cgggcctcgt cggtcatccc gccgtccagg tcagcccttt cggccgccac
     2821 ggtagatctc gtcgagcagc tcctcggggg tcgtcacgcc gggccggcgg gcgagcgccg
     2881 cgccgatgat cggggccgcc tcgctcggcc ggtagccgag ctgcttcacc aggatgtcga
     2941 agaccatacc gcggagcgct tcctcgtcgg gcgcggcggc ggcggcgacc ggctccgggc
     3001 tcgccgcctc tcccttcgcc agcgcgaact tggccacctt ggcctggagc tgcgccacga
     3061 tgttcttggc cttctgcggg ccgatgccgg gcagccggcg caggtagccc tcgtcctgcc
     3121 gcgcgatggc cgccgcgatc tccccgaccg gcgcggccag ggagcgggcc gctaccatcg
     3181 ggcccacgtc cttcacggtg atcagcttct cgaagaactc gcggtccagc tccgaggtga
     3241 agccgatcag cacgggcctc ggctggttct gggtggcatg gtagtggatc tcgagcgaca
     3301 cctcgtcggc cgcgtccccg tcggcagcct tggcgtgcag cagctcctgc tgagtgaccg
     3361 gcgggacgaa gacctcgtag ccgacccccg ccgcctcgac gaccacgcga tcctcgagct
     3421 tgcgcctgag cttgccacgc aaggacgcga tcacggtcgt ccggtaaccc tcgacgagat
     3481 gaaccggcgc acggcctcgc tcgtcggttg tccgccattg ggggagtgcg ggggccattt
     3541 cgccgcgacc ttccccgtga tccgcgccat cccggttgcc gccagcccca gcgcatcggc
     3601 cacgtgggac gggcgcggca gctccttgag cccgagcacg gtctggaccg cgcgctgcat
     3661 ctggcccttg cccgccgcgc cgttgccggt catggcgcgc ttgacctcgg acggcgccag
     3721 cgcgagcacg cccaccccga gctggcgggc ggccaggtag atcacccccc gggcgtggcc
     3781 catgaggatg gcggtgcgcg ggaacttgta ctcggtgtag aggtcctcga ccacgagcag
     3841 tccggggctc tgcagctcga tgatccggtg cacggcccgg tgcagggcgt tcagccgggc
     3901 ctccagcggc tgtccggtgg tcgtgctgat gacgccggcg tcgcaaaccg acacgccgcc
     3961 ggggccgacc tccagcacgc cgaacccggt ggcgacgagc cccgggtcca cccccatgac
     4021 gcgaagggcg ctaggccgcg gtgatggcgt cgatgacctc gtcggcgatg tcgtagttcg
     4081 aatacacgga ctggacgtcg tcctgctcct cgagggcctc gatcagccgc agcaccgccg
     4141 cggcctcctt accctctacc ttgaccgtgg attgcggcac catgtccacc cgcgcctcga
     4201 gcaccggcat cccctgccgc tcgagtgcct ggcggaccgc ctccatttca tcaggactcg
     4261 cggtgacctc gaacactttt tcgacctgct tgacgtcggt cgcccccgag tcgagggcct
     4321 tctcgagcag atcgtcctcc ttcaccttct gggcgtccac ctcgatcatg ccgcggcggt
     4381 cgaacatcca ggccacgcag cccgaggtgc ccatccgccc ctcgttcttc tcgaagatgt
     4441 gtcggatctc gggtccggtg cggttcttgt tgtcggtcag cgtccgcacc atgaccgcca
     4501 ccccgcccgc cccgtagccc tcgtacgaga tctcctcgta cgactcgccg gggagctcgc
     4561 cggtgccctt ctggatggcc cgcttgatgt tgtcggcggg catgttggcc gcccgcgccg
     4621 actccgtggc cgccttcagg cgcaggttgc ccttgggatc gcccccgccg ttcttcgccg
     4681 cgaccgtgat ctcgcggagg atcttcgaga acagcttccc gcgctggacg tccgtcttgc
     4741 ccttcttccg cttgatctgc gaccaccgtg agtgtccaga catggctatc ttccttccgt
     4801 gactgccttc ttgtattcgc tcggctcgcc agcggctgcg cctcgcactt ctatgactgc
     4861 cttcttgtat tcgctcggct cgccggcggc tgcgcctcgc acgacttgga cccgctccag
     4921 gcgccgcgcc cgccgttcca gggcgccgac ccagcggcgc tgctcggcgg gggtttcggg
     4981 cgtgaagggc gggcacggct gggggcgcaa cggagcgctc ggatcggagc ccaggtgcac
     5041 gaaggtgaga taggcggtgc aggcatgctt gatctcaccg gtccacggca cctcgctcag
     5101 caccttcacc ccgatctcca gcgacgatct ccccacgtgg ttgaggcccg cctggaacgt
     5161 gacgacctcg ttcgactgga ccggcgcata gaagtcgagc gcgtccaggc acgcggtcat
     5221 caccaggccc cggcagtacc gcatggagag aatcccgccc gcctcgtcga tgtcgagcag
     5281 gagcttgccc gcgaacatgg tgttgccgaa cagcaggtcc tccggcagca ccgaccggca
     5341 ggactcgaac cgccagcgga gctgctcggg cgcgtcgtcc tccacctcgc gcatccgctc
     5401 ggcgcgccgg gcgaagcgcg ccagccgccg ggcgcgccgc tcgcccgccg ccgcgtgcat
     5461 ggcctcctcg tcggatccgc gcggcaccac ggcctgcggc accggcaccg gcttgacgtg
     5521 ctcgtccacc ttgacgaaca cgaggtggga cgatagcgtg agcgcccgct cgccggtggc
     5581 gggcttctcg gcgtagacgc gcaccccgac ctcgagcgag caccggccca cgtactccac
     5641 ctgggcccgc agcaccgcca cctcccccac cttgaccggg tgcaggaagt cgatgtcgtc
     5701 catcgctccc agcaccacgt tgccccgcgc gacccgcgag gccgcgatgg tgccgcaggt
     5761 ggcgatccac tgcatcatgc ggcccccgtg gatctgcccg ggcgcccccg cgtgctcggg
     5821 gaacacccag ttcaccatct cggtagccgt gtcggagatc gagagcatgg tcagcgccgg
     5881 atgtcgcgta agtctcgcat ttttctacca cggagggccc tccaagtcca gccgcggcgc
     5941 ccccggaggc gttgacaatc gcgcggctcg gccctagggt tggggccacg tcagggggcg
     6001 agaagggaca ccgtaccaga ggcaccggcg ccgggaggcc cacatggaat tcgagtactc
     6061 gaagaagacc aagatgtaca tggagcagct caccgacttc atgaacaagc acgtcgtgcc
     6121 gaacgagcag gtcttcgccg accagctcaa ctcgggcccg acgcggtggc agatccctcc
     6181 gatcatggag gagctcaagg ccaaggcgcg cgagcgaggc ctctggaacc tgttcctgcc
     6241 cgagagcgag cgcggcgccg ggctcaccaa cctcgagtac gcgccgctct gcgaggtcat
     6301 ggggcgctct cccatcgcgc ccgaggtgtt caactgctcg gcgcccgaca ccggcaacat
     6361 ggaagtgctc gagcgctacg gcacgccgga ggacaagaag cagtggctgc agcccctgct
     6421 cgagggcaag atccgctccg ccttcgcgat gaccgagccc aaggtggcct cctcggacgc
     6481 caccaacatc gaatcgcgca tcgagcgaca gggcgaccac tacgtgatca acgggcacaa
     6541 gtggtggacg tcgggcatcg gcgacccgcg ctgcaaggtc ctcatcttca tgggcaagac
     6601 ggatccgaag aaccccgaca agtacaagca gcagtccatg atcctggtgc cccgcgacac
     6661 gcccggcatc aaggtggtgc ggatgctgac cgtgttcggc tacgacgacg ccccccacgg
     6721 tcacggcgag gtgctcttcg agaacgtgcg cgtgcccgcc gccaacatcc tgctgggcga
     6781 gggccgcggc ttcgagatcg cccagggccg cctgggcccc ggccgcatcc atcactgcat
     6841 gcgccagatc ggcgtggccg agcgcgcgat cgagctgatg tgcaagcggg ccacccggcg
     6901 gatcgccttc ggcaagccgc tggccgacaa cgacatcacg ctcgagcgga tcgcggagtc
     6961 gcggatcgag atcgaccagg cgcggctgct cgtgcttcac gccgcgtaca tgatggacac
     7021 cgtcggcaac aaggcggccc ggcaggccat cgcggagatc aaggtggccg tgccgagcat
     7081 cacggggcgc gtggtggagc gcgcgatgca gctccacggc ggcggcggcg tgagccagga
     7141 gttcccgctc gcgtccatgt gggcgcattc gcggacgctg cggttcgccg acgggccgga
     7201 cgaggtgcat cggcggcaga tcgggcggct cgagcttcgt aaacacagct agcgttaagc
     7261 ggaggagagc gggcgggggc gcggggcggc ggggagccgc ctctgacccc gacccgctcc
     7321 ctcttcacct cgcggtcagg agttcccagg gagcaagcac agcagcggca ggaggcggag
     7381 gcccttcacc gctgggcgct cgggacgggc gccgccgccg cgggcggcgc gtcgatccag
     7441 acgcggtcga agcggggatt gccgctcggg tgcaggcgca ggttccgcac ctccgtgcgc
     7501 gtcgccgccc agtgcagccg cacgtagagt ggcacccacg gcatctcctc ggccagcatg
     7561 gcctgggcgc gcacgtagag gcgctgccgc tcgggccgga aggacacctg gctggcccgg
     7621 atgaggaggt cgtcgagggt gcggttgcgg tagaaggaca tgttcagcgc cgcgtgcccc
     7681 ttggtcgcgc cctcgctgct ggtcagcggg tagagcagga aatgcggatc gcccgcctcc
     7741 acgcgggcct cgagcagggc catctgatgc tggccggtgc gagccagctg gagcgccgcc
     7801 tcgggcgcct cgacctggat gggcaccgag atgccggcgg tggccaggga ggcgcggatc
     7861 gcctcggcca cccgcgcctg gtccgggcgc ctgtcgccgt cgctgatcag cagaccggcc
     7921 gacgtcccct gcgggaagcc tgcctccgtc agcaggcgct tggcgttcac cgggttcgcc
     7981 tcgagcacgg gcgagccgtc gcgacggccc cagaccccca gcggcaggaa cgagagcagc
     8041 gggaccccgg tcggcgcgac cgcgggggcg agcacgcccg gatcgagcgc ggccgccacc
     8101 gcccggcgcg ccttgagccg gctgaagggc tcgcgctccg cgtcgagcgc gaggtagccg
     8161 atgcgccagc tcgggatcga ctgcgtgccg gcgggccggg acggggcgac cgaggtgagg
     8221 agcacgtcca ccgcctgcgc ttccagcatg gcctcaccgc gcccatcctc gccggcgtcc
     8281 aggaagacca gccgcgtcga ccggggcggg ccgccccaat agcccggatt cgcgtcgagc
     8341 accacccgcc cgaggctcgc ctcggtgagc gcgaacggtc cggtgccgac ccaccgctgg
     8401 cccgcgctgc tcgccagcac gatcgagaag gcgggatggg ccagcaccgc gggcagcggc
     8461 gcgtagggca gcagcaggct gatctggacg gtgcgcgcgt ccgccgcctt gacttccttg
     8521 acgacccccg gcgtaccccg gatcagccgc ggggcggcca ggttggggct cggggcgtgg
     8581 ggcgcggtgg gctggatgac ccggtcgagg ctctccacca cgtgctgggc ggtcaggacg
     8641 gtgccgtcgt gaaagcgcac gccgtcccgc agccggaagg tccacacgag accgtcgcgc
     8701 gacaccgacc attgggtggc cagccccggc tcgatatcgc tcgtgacgtc gcggtactgc
     8761 accagggtgt cgaagatttg tcgcgcgatg acggaaacgg gcccttcgag cgcggtggcg
     8821 gggtcgagcg gggccggcac cgacggcagg cccacgcgga ggacgccacc ctgagcccag
     8881 gcctcgccgg ccagcgtgag gacgaaggcc agcgtgatcg cgatgcgccg gcacgcgctc
     8941 cacatgatcg tgttatacga cggaatcccg ccgctgcgcg atgcgtagcc actttccccg
     9001 ccgctgatta ttcgatgggc cctcgtgctc ccacgcgcgg ccgcggctcc gctcgaacgg
     9061 cgcgggaatc ggcgtggtcg gcgggccgtg cgaatggcac cgaacctgct tcatcccgtt
     9121 gcgggcccgt cgggggcact ccggaaccgg gtcgggtgag ccacggttta cacggtgaga
     9181 gcgatagggg atcgccgcac cgcgaatcgg agaaagtgaa atgaaatcaa tggctcccga
     9241 gggcaagatg aggccaattc gcttgactta gcgcgaagcg gactcctata ctgaggtcac
     9301 ctttcggagg cttgggcggc gcgcgatgga gcgctggaag tgcccgcggg ttcacccgga
     9361 aaggaaatgg gcgaacatga aggagttcga caagaagggc aatcctagcc gagagcccgc
     9421 gtccgaaggg atgaagggcc tctcgaaatt tctccccatg accgactcca actaccggcg
     9481 gcgcctcacg gagtacgagg tccaggtcca ggacctccag gcctacgtcc gctcgctcga
     9541 gacggagacc gggcggctgc gcaagaagct ggaggacgcg cccaaggagt tcatgatcct
     9601 ggagaacaag ctgcgcgagg ccaatcgcca gctggtccag gcgttcaacc agaacgagaa
     9661 gctcgtcaac gcgctctacg aagcgcggga gcagatcacc tcgctcaagg aagaggtgga
     9721 caagctctgc gcgcccccct cgacctacgg ggtctacctc tcggtcaacg aggacggcac
     9781 cgtcaacatc ctgtcgcagg ggcgcaaggt gaaggtcaac ctccacccct cgatcaaggc
     9841 cgaggacatc aagcccggtc aggagctgat cctgaacgag ggcctcaacg tggtggagac
     9901 cgcgggctac gagatccagg gcgaggtggt catcctcaag gagctcctcg acgcggagcg
     9961 ggccatcgtg acccagcggg cggacgagga ccgggtaggc atcatcgcgg agccgctgcg
    10021 ctcggcgaag ctcaagatcg gcgaccacct gctcatcgac gccaagagcg gctacctcct
    10081 cgagaagctg ccgaagagcg aggtcgagga cctcacgctg gaagaggtgc ccgacatcga
    10141 ctacgaggac atcggcgggc tcaccagcca gatcgagacc atcaaggacg cggtcgagct
    10201 gccgtatctc tacgccgact actaccggga gcat
//
LOCUS       DASUFA010000304         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_401825, whole genome shotgun sequence.
ACCESSION   DASUFA010000304 DASUFA010000000
VERSION     DASUFA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401825"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFX87_11340"
     CDS             <1..597
                     /locus_tag="VFX87_11340"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEX4995560.1"
                     /translation="RTASGFTLLELIIALGIVAVLLVITFGGLRVGLAAWQRGEERAA
                     TLDHARSLVLLLEGTLAGTFPYRATLAEGEQTRIIFDGQPDRLTFVTATALFPSEMPI
                     AFTVVSLSAEAGALALRQQPLPNQGALDRLPPVLVDPETTAVRFRYLGPEPEAWQEQW
                     NTSEARPLPRAVEITLLTGAGGPRVAQAFTVPIRASVQ"
     gene            594..1532
                     /locus_tag="VFX87_11345"
     CDS             594..1532
                     /locus_tag="VFX87_11345"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HEX4995561.1"
                     /translation="MKTQAGFALLAVMLVLTLLAVVVTEFTFSARLEASMVRAYRDGV
                     LASHLAESGVEQAIREILSQAQIAAPDRDGQLIFYRALPGQTTAARLPALPRTRVGLG
                     SGEFSYRITDEAGRLSVNGPAVRIDRLLAALGLDQQQRDIIRDSLEDWRDANEISRLN
                     GAESDFYLRLAVPYRARNANVQDTAELLQIRGVTRELYFGTAERPGLHDLVSAVGLST
                     VNVNTASAPVLKALGLSDAEVANVIETRARVPYTAVPGPLAGRGLTVASATFRIEAVG
                     VAGGIPRSRVVAVVQRGGPQGPLGVRILSWRPGSAE"
     gene            1529..2905
                     /locus_tag="VFX87_11350"
     CDS             1529..2905
                     /locus_tag="VFX87_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX4995562.1"
                     /translation="MIAPRVGLFLGDGRLTVVAVTGRDRVERFVIADSEDPAGALAAE
                     LGARRLATRRIRVGLDRRLAVVKIIQLPRAAGGNLAQMVGFDLERHVPFPPEDARFDW
                     TELPTKAEKPRRVLVAACERRVVERPLGLLAAAKRRPAALTVACHELTALLRRALPSE
                     RVVWAHRHGSSTDLLFLRGRRLLMSRTVAVEDPEGLAREIDRSLPLVQWTRYHAVWIS
                     GDEADAWRAAPGVAAELDAPVAAPPYAPSRAALIARLPEKDPGAALLALAVACGPRRP
                     LLDLLPAEARPWTLSREQMISAGLAIITVLLGLTLAYTHALKTEHYLARLTQEIRRLE
                     PEAKAAEGLSAELARKKRILASLRAAEGGRVQALPVLRELTETLPASAWVQSLTLSRE
                     GVELTGQADAASALIPLLEASSWLERVEFTAPVTKAQSKEQFRLRAGWETPSPLLPSA
                     GRRPGEGG"
     gene            2909..>3371
                     /gene="gspM"
                     /locus_tag="VFX87_11355"
     CDS             2909..>3371
                     /gene="gspM"
                     /locus_tag="VFX87_11355"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX4995563.1"
                     /translation="MPAFTRRERTLVGLGAVAAVVIGGCLFLVEPLLTRGRDAEATVP
                     AREASLERRRLLVGQHPRLVQELETVTQQLEAESGRLLRGPTAPLAASELQKIVKDLM
                     IGSNVEVRSERVLPVSDRDGLQEVPIEMTIAGSIRDLVAALARLERTGCLLV"
BASE COUNT          440 a         1250 c         1189 g          492 t
ORIGIN      
        1 cgcacggcga gcgggttcac gctcctcgag ctgatcatcg ccctcggcat cgtggcggtg
       61 ctgctggtga tcacgttcgg agggctgcgc gtcgggctgg cggcctggca gcggggcgag
      121 gagcgggcgg cgaccctgga ccacgcgcgc agcctggtgc tcctgctcga ggggacgctg
      181 gcgggcacct tcccctaccg cgcaacgctc gccgaaggcg agcagactcg catcatcttc
      241 gacggccagc cggatcggct gaccttcgtc accgccaccg cgctcttccc gtcggagatg
      301 cccatcgcgt tcacggtcgt gagcctctcg gccgaggcgg gggcgctcgc gctgcgccag
      361 cagcctctgc ccaaccaggg cgctctcgat cgcctgcccc ccgtcctggt ggacccggag
      421 acgaccgcgg tgcggttccg ttatctgggc ccggagcccg aggcgtggca ggagcagtgg
      481 aacacgagcg aggcgcgacc gcttccacgc gccgtggaga tcaccctgct caccggcgcg
      541 ggcgggcccc gcgtggcgca ggccttcacg gtgcccatcc gggccagtgt gcaatgaaga
      601 cccaggccgg cttcgccttg ctcgcggtca tgctcgtgct gacgctgctg gcggtagtgg
      661 tcaccgagtt caccttctcg gcgcgcctcg aggcctccat ggttcgcgcg taccgcgacg
      721 gggtgctggc ctcgcatctg gccgagtcgg gcgtcgagca ggcgatccgc gagatcctga
      781 gccaggcgca gatcgccgcg ccggaccggg acgggcagct gatcttctat cgcgcgctgc
      841 cggggcagac cacggccgcc cggctgcccg ccctgccccg tacccgcgtc ggcctggggt
      901 ccggagagtt ctcctaccgg atcaccgacg aggccggccg gctcagcgtc aacggtccgg
      961 cagtccgcat cgaccgtctc ctcgccgccc tcgggctcga tcagcagcag cgcgacatca
     1021 tccgcgattc gctagaggac tggcgggacg ccaacgagat cagccggctc aacggagccg
     1081 agagtgactt ctacctccgg ctcgcggtgc cctatcgcgc ccgcaacgcc aacgtccagg
     1141 acacggcgga actgctccag atccgcgggg tcacgcgcga gctctacttc ggcacggccg
     1201 agcgtccggg tctgcatgac ctcgtctccg ccgtcggcct cagcaccgtc aacgtgaaca
     1261 ccgcctcagc gcccgttctc aaggcgctcg gactctcgga cgccgaggtc gccaacgtga
     1321 tcgagacccg cgcgcgcgtt ccgtacaccg ccgtgcccgg gcccctggcc ggccggggcc
     1381 tgaccgtcgc cagcgccacc ttccgcatcg aagcggtggg tgtcgcgggc gggattccgc
     1441 gcagccgcgt ggtcgccgtg gtgcagcgtg gcgggccgca gggccctctc ggcgtcagga
     1501 tcctttcctg gcggcccggg agcgcggaat gatcgccccc cgcgtcggac tcttcctcgg
     1561 tgatggtcgg ctcaccgtcg tggcggtcac cggccgcgac cgggtggagc gattcgtcat
     1621 cgcggactcg gaggatccgg cgggggcgct cgcggccgag ctcggagcgc gcaggcttgc
     1681 cacgcgacgg atccgcgtcg ggctcgaccg caggctggct gtggtcaaga tcatccagct
     1741 gccgcgcgcg gccggcggga atctcgccca gatggtgggc ttcgatctcg aacgtcacgt
     1801 gcccttccca ccggaggacg cccggttcga ctggacggag ctgccgacca aagccgagaa
     1861 gccgcgccgc gtgctggtgg cggcctgcga gcgccgcgtc gtcgagcgcc cgctcggtct
     1921 gctggccgcg gcgaagcgcc ggcccgctgc cctgaccgtg gcctgtcacg agctgacggc
     1981 cctcctgcgc cgcgcgctcc cgtcagaacg cgtggtctgg gcccaccgcc atggcagcag
     2041 caccgatctg ctcttcctgc gtggtcgccg cctcctgatg agccggactg tggccgtcga
     2101 ggatccggaa gggctcgcgc gagagatcga ccgcagcttg ccgctggtgc agtggacccg
     2161 ttaccacgcc gtctggatct cgggagacga ggccgacgcc tggcgcgcgg cccccggcgt
     2221 ggccgccgag ctcgacgcac ccgtggccgc gccgccctat gcgccctccc gcgctgcgct
     2281 catcgccagg ctcccggaga aggatcccgg cgcagccctg ctggcgctgg cggtggcatg
     2341 tggacctcgg cgacccctcc tcgatctcct gccggcggag gctcggccgt ggacgctctc
     2401 ccgcgagcag atgatcagcg ccggcctggc aatcatcacc gtgctgctag gcctgaccct
     2461 cgcctacacc cacgccctca agaccgagca ctatctcgcg cgcctcacgc aagagatccg
     2521 ccggctcgag cccgaggcga aagcggccga gggcctatcg gccgagctgg cgcggaagaa
     2581 gcgcatcctt gcgtcgctgc gggccgccga gggcggccgc gtccaggcgc tcccggtcct
     2641 gcgagagctg acggagacgc tgcccgcgag cgcgtgggtg cagtccctca ccttgagccg
     2701 cgagggcgtc gagctcacgg gccaggccga cgcggcgagc gccctcatcc cgctgctgga
     2761 agcctcgagc tggctcgagc gcgtggagtt caccgcgccg gtgaccaagg cgcagagcaa
     2821 ggagcagttc cgcctccgcg ccggctggga aacccccagt ccccttctcc cctcggcggg
     2881 gagacggccg ggtgagggag gctaagcgat gcccgccttc acccgtcgcg agcggacgct
     2941 ggtcggcctg ggcgccgtgg ccgcggtcgt catcggtggc tgcctcttcc tggtcgagcc
     3001 gctcttgacc cgcggccgcg acgcggaggc caccgtgccg gcgcgtgaag cctcgctcga
     3061 gcgccgccgc ctgctcgtcg gccagcaccc tcgcctggtc caggagctgg aaacggtcac
     3121 gcagcaactg gaggctgagt cgggtcgcct gctgcgcggc ccgacagcgc ccctggcggc
     3181 ctccgagctc cagaagatcg tcaaggacct gatgatcggg agcaacgtcg aggtgcggag
     3241 cgagcgcgtg cttccggtct cggatcggga cgggctccag gaggtgccga tcgagatgac
     3301 catcgcgggg agcatccgcg acctggtcgc cgcgctcgcg cgtctcgagc gcaccggctg
     3361 cctgctcgtg c
//
LOCUS       DASUFA010000305        18196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_295232, whole genome shotgun sequence.
ACCESSION   DASUFA010000305 DASUFA010000000
VERSION     DASUFA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 18196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18196
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295232"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..760
                     /locus_tag="VFX87_11360"
     CDS             56..760
                     /locus_tag="VFX87_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995564.1"
                     /translation="MLELSQVTAGYGAFTALWDVSLRVNQGEAVAVVGPNGAGKTTLL
                     RAISGLVAPRAGRVTFEGAELAGHPAYDIVAHGIAHVPEGRRLFPQLTVAENLKMGAF
                     LSAARAHFHESLERVYTLFPVLAERSTQRAGSLSGGEQQMLAVGRALMSRPKLILFDE
                     PSMGLAPVMVLRLFDLIRRVRADGYTILVVEQNVRQVLKLVDRAYLLEVGRIKMEGRA
                     ADLAEQDFVRQAYVGL"
     gene            763..1629
                     /locus_tag="VFX87_11365"
     CDS             763..1629
                     /locus_tag="VFX87_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4995565.1"
                     /translation="MIEPIFILEAVVNGILLGGVLALLALGLNLIFGVIDIVWIAYVD
                     LVALCMYAVYFLVTLYGWPLWLAGLGAIAVGAGLGVLVHLLIITPILSSPPINQLLAT
                     GGLLFFIQSVATLLWSTDHRSVRVSLPTIEVGGIFISFSRLLAFGLSILAMIALYLFL
                     KRTYTGTAIRAVSQDREAIALMGARPQRLYIVTSAVGGAMAGVAGALLIIQYAVHPYF
                     GGAFGPLTFMICVLGGLGNMVGAFFAAFIMAQVISVGGVMVSTEMGYVIAFVIFIVLM
                     FVRPGGIFAKRG"
     gene            1626..2639
                     /locus_tag="VFX87_11370"
     CDS             1626..2639
                     /locus_tag="VFX87_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4995566.1"
                     /translation="MTIAFRVLGLVALVAMALIPFLPLGAAQEYIWHVIIQIFLWSFV
                     GNAWSLMGRFGLVSLGHGAFLGVGAYATVLLWNFYGMTPWAGVVLAVLLTVALALVIA
                     YPCSRFQVVGHYFALVTLAVGEVVRLWIVAERDWTGGSLGVSLKTGGTASGLWTLQFA
                     DKRVFYYVGMAAWLFGLWVWWRVDRSMARSAMEAIGEDETAAASVGIRVTRFKMGITA
                     LSAALTALGGALLALYLAYTNPDTLSGIGVSLRIVFAVVLGGMYSLLGPFVGTALTIA
                     LSEYLRIYFGIKLIGIAETIYGLLLIIFIIFLPAGIYGSIRDALFRRRPAGSAGAAVR
                     APS"
     gene            2636..3157
                     /locus_tag="VFX87_11375"
     CDS             2636..3157
                     /locus_tag="VFX87_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995567.1"
                     /translation="MTRLSLGAGPAALTLATIIAAGCGGNPALVVRGGQSEAQIAQDR
                     AQCLSFVQAHTETTPELAEAACLVNRGYRAPLPLAQGPAGIGSLYATANRDANVIVAD
                     FQGCRVEAFNTPMPENKEKKAPSGIFSSFFGQLFPRGFITKARTPDEWALQTFAACLN
                     RRGYTVSDVTPAR"
     gene            complement(3205..3960)
                     /locus_tag="VFX87_11380"
     CDS             complement(3205..3960)
                     /locus_tag="VFX87_11380"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEX4995568.1"
                     /translation="MNDGEGEPSEPIPGAVGSRDAMLLVLTCAAGLVDAISYLGLGHV
                     FTAMMTGNTVLLALAIGQGEVMAALRSTLALAAFSAGAAGGAMVLLRGERRGEWPPIV
                     TATLALEGVVLGVFGVIWYVEGPAPRSDGVVLVLIALSGLAMGVQAAAVRHLGVPGVA
                     STYITGTLTSLMAELVSWRRPRGAPSSGETSGAALRHLRLLAAVFLVYGAGALVGTVL
                     HAGSSMLLAWLPFVCVAAVVVTGFVRHREPPPG"
     gene            4065..4973
                     /locus_tag="VFX87_11385"
     CDS             4065..4973
                     /locus_tag="VFX87_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEX4995569.1"
                     /translation="MARWRVVPDEEPARAVAALADQVERDGGSVLAIYREPVGDHWQI
                     FCLLPLEKVEPTPYQRDLSPTHVKRLQEVVKKIDRFVDPVVVMSPRPGVYWTPNGNHR
                     RTVLIKLKAKVIPAILVPEPEVAFQILALNTEKAHNLKEKSLEVIRMYRGLVDEEPQS
                     GEEDYTFQFESAHFVTLGLLYEQNKRFAGGAFAPILRRVDKFLKGHFPKTLKEREERA
                     ALVRDADEALVAVVARIKKRGINHPYVKNFVLARTTPLTRQRKTLPGFEQTFKKLVEN
                     IEAFDVAKVRYEDIQRSAIMGAPAAG"
     gene            4983..5294
                     /locus_tag="VFX87_11390"
     CDS             4983..5294
                     /locus_tag="VFX87_11390"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX4995570.1"
                     /translation="MARPTKLADAEVTQRLAALPGWRLEGGKLHRELTFGDFTQAFAF
                     MTDVAREADALDHHPEWFNVYNRVVIDLATHDASGITTLDFELARRAEDLAGRRARPA
                     P"
     gene            5291..5986
                     /locus_tag="VFX87_11395"
     CDS             5291..5986
                     /locus_tag="VFX87_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995571.1"
                     /translation="MTGRVAVVTGGTGALGQAITPHLLEAGGIVAIPYAVPEELTGLE
                     ARLAPELRARLLALPADVTREDAMGKFVQAVRDRHGRVDGLVNAVGGFAGGDLLATSL
                     AEWDRMMALNLTSTVIACRAVLPVMIAGGGGRVVNIASRAVLSPQGGFIAYTVSKAGV
                     ITLTQALSQEVKKHGVTVNAVLPSTMDTPGNRRAMPDADRSEWVSTRDVAHVVAFLLS
                     ERAAAVTGASLTV"
     gene            6000..7400
                     /locus_tag="VFX87_11400"
     CDS             6000..7400
                     /locus_tag="VFX87_11400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4995572.1"
                     /translation="METFDLVVIGAGAAGLTAARTAAGLGARVALADRGPLGGLCVNR
                     GCIPKTALVAAGRVHRQVREAGGFGTFSGPVRLDWDAVQQRQDEIVNAHHPSAADLEK
                     RGIRVAIGEVRFTDPHMVEVNGRPLGAERFVIAAGSQPVIPEVPGRELLITSDQLLFL
                     TEFPASLTFIGAGPVALELAGAFNDFGSRVTVLARDAEILPGLDADVARNLRKHMEER
                     GVTFRLQTRVTGLARATEGVRIAFLSAGMANEMTSRQVCAAVGRRFHPRTIGAARIGL
                     EMGRLGLRTNAHLATSVPHIYAAGDAAGNRQLSTVAAYEGRIAALNALRGDTERADEA
                     VIPQVLSTTPTVGLVGVAYGEAPAHGIHAAVARHDALGSSYSVVTGEDAGYFKLVFDE
                     DSQRLVGAQMVSPAAEELIQLCALAIRARVPASLVETQLSVHPSRTDHLMRAFAPEPG
                     AVPRPVEGADVVESPP"
     gene            7397..8770
                     /locus_tag="VFX87_11405"
     CDS             7397..8770
                     /locus_tag="VFX87_11405"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4995573.1"
                     /translation="MIRPASRRAVPWLAVALLVSACAGSGARSAPPAPGAAEAELPPP
                     VRQVLRNGMRLIVQDHRAADIVAVYLFVGVGVRYEAPDQLGYAHFQEHMLFKGTDRFG
                     PGYVDRTVEGVGGRSNAVTSFDYTEFHLVVPSEATATGIELLADMAFRSSFDPDEVGR
                     EREVIFEEARIEQDNPRSAVVRQLYGLVFAGNPYGRPVLGTRTTMQAATRERLRAFNR
                     RYYVPENMTLVVAGPVDPVAVRGIVDRTFGRMPATSYKPTPAPAPAPLTGGVRRTVER
                     DEQQAHLALGWQAPRSDDANGDAVDLLTTILAGTESSRLAQRLRDRERLVSTVTMSYA
                     ALMGGGIVSLRAELEAKDLERVERIILEEIARMQESGPTEEERRLAVTKFEAQHAFDT
                     ETSEGLAYAYGLAETTWTLDAELRYVDRLRRITREQIRDAARRYLSRTDYARLAFVPK
                     PGTPSTR"
     gene            8770..10068
                     /locus_tag="VFX87_11410"
     CDS             8770..10068
                     /locus_tag="VFX87_11410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4995574.1"
                     /translation="MRGRALLVAGLLALLAAPAWAQSPVVSRARLPNGLTVLVRENPT
                     APVVGISLMVRMGTRWETADNAGISNLVQLMIVRGTTTRDGGQIVEAADAMGGSIEAT
                     GDVDYSEVAATALSRHWVEMLELVADVALRPTMPDGVVVAVRDFLLRQVRNRGDKPYD
                     VALDALTARVFGRHPYAWDPLGLKESLERLDRGALLAHYKRHYVPGGLVLAVSGRVKT
                     AEVMAQATRLFGPMPAGAAPAAAPPPPPAPAPWREARVVPGAQAQILMGALAPALTDP
                     DYPAVKVLATVLGGGMAGRFFSELRDKQGLAYTTAAQYPARVDTSFFLVQLGTAPENV
                     ARAEAALRAQLERVQRERVSNEELRVAKAYVLGNLAMDRRTNARQAWYLAAYEESGVG
                     YEFLDRYMTAVRAVTAADVQRVARRYLAVLRTVIVQPTAP"
     gene            complement(10079..10669)
                     /locus_tag="VFX87_11415"
     CDS             complement(10079..10669)
                     /locus_tag="VFX87_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995575.1"
                     /translation="MRHRIFFPLLLGSVILSTGCAATHREWEVWRAHPTHYATTNHLA
                     FSMKNTVGAAPQVTPELMDQAKGEGWWGRNTPFAMPRADVAGRWEGTWSGYGMSRTLR
                     GGVAQAVLTVDGATGQGRLVLVDARMAEGVPLSLRENSSFGSPIELSVSETEMWVNGT
                     EPGRPFAATFTLQGDKLVGQFLYTSSPVRIEMTRMR"
     gene            complement(10737..11501)
                     /locus_tag="VFX87_11420"
     CDS             complement(10737..11501)
                     /locus_tag="VFX87_11420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4995576.1"
                     /translation="MQFPGGLPPRARALHRSPGGHVLRALLIDLDDTLLDYSGGMDEC
                     WLHACEALAGPAGIDPPGLVAAIAETRRWFWSDPSRHRTERADMLRAWTRITAHALGR
                     LGRADEALAACIAEDFAERRWERMTLFPGVTPALEALRARGVPLAMVTNGDASHQRRK
                     IERHDLERFFDVIVVEGEMGVGKPEEVVYRYALSKLGVKAEEAWMVGDNLEWDVLAPQ
                     RLGLRGVWVDAPGQGLPKSCSVAPHRIIRAFPELLE"
     gene            complement(11432..12373)
                     /locus_tag="VFX87_11425"
     CDS             complement(11432..12373)
                     /locus_tag="VFX87_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX4995577.1"
                     /translation="MADLVNEFSWSRTRDNVFQDCRRRYYYQYYGAWGGWDADSDPLV
                     RRLYVLKQLATRQMWAGRLVHEAIERSLLALRDGHGLSEASLIEDTVRQMREEWKGSR
                     AGVYRQAPKRPSLFEHEYGVAIRDSEWQALRDHVVRCLRNFHRLPVLTDIKRTPTERW
                     VFIEDIGSFPFEGTRVFSAPDFGYWSHADRLQLLDWKTGGGGEGAGLQLGGYALYALE
                     VLGVDLGRVDLLEVNLREGKVTTHPWDEESLDRVREHIRLSVRSMKAYLKDPERNLAE
                     ESGFEKTEELRICRWCNFRAVCRPELAPFTAPQEVTS"
     gene            12543..14174
                     /locus_tag="VFX87_11430"
     CDS             12543..14174
                     /locus_tag="VFX87_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995578.1"
                     /translation="MRIIAGALIIVGLATLTAGPIRAQGKATERLGTVRFVTSCAPAV
                     QPRFDRAVALLHSFHLDAAVGAFTEVATADPACGMAHWGVAMAWLGNPLAGPPSARGL
                     KEGSAAVDRAKAAGAKTDREREYIAAIETFYKDADRVDHRTRAVAYERAMEQIAARHP
                     ADREAAVFYALALNITLDPNDKTYANQLKAAGILEKVFAEQPDHPGVAHYLIHSYDFP
                     PIAAKGLPAAKRYAGIAPAAPHALHMPSHIFTRVGNWQESIETNRASAKAAADTLAAG
                     GSQTGLRSYEGLHAYDYMMYGYLQLARDREARALVEEVTATRKLNVDHFAGAFALAAI
                     PARYALERGQWADAATLTPTPSDLAWNRFPQAESIVWFARGLGAARSGNPVRAREDLA
                     RIEALRDSMTAAKSLYWAHQSEVQRLAVAAWIARADGRNEEALSLMRASADAEDATEK
                     HPVTPGSIKPARELLGEMLIDLGQPAEALKQFEASHRVEPNRFQGLYGAARAAELAGD
                     RAKARTYYGRLVALGQGADGDRPELKQAKIFIATK"
     gene            14340..15179
                     /locus_tag="VFX87_11435"
     CDS             14340..15179
                     /locus_tag="VFX87_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4995579.1"
                     /translation="MPLAPRYLAALVPVVLLALVLATFGSLDAQAGKIEVLWLGQSAT
                     RITTVTGKVIVIDPFLTQNPKTPAQYKNLDALGKVDLILVTHAHGDHLGDGPELAKKH
                     KAPLWGPAGLDQSLLTLGVLPAELAPRMNKGGTITPLGPDIKITMTRAEHSSELVWTN
                     PATGKPETHVGGEPAGFLIEFENGFKLYHMGDTGVFADMKWIGEYYKPDVIMIPIGGH
                     FVMSPKDAAVATEMIKPKFAIPIHYGTFPPLKGTPQEYMQALGATAVKVFPINPGDKL
                     MFP"
     gene            15389..15583
                     /locus_tag="VFX87_11440"
     CDS             15389..15583
                     /locus_tag="VFX87_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX4995580.1"
                     /translation="MPFVSIRIYEGWGKERLNEIAKRVTAAITDVTQLPKEAVWVVFE
                     EVDPPDWYVGGKAGERMAKK"
     gene            15648..16541
                     /locus_tag="VFX87_11445"
     CDS             15648..16541
                     /locus_tag="VFX87_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4995581.1"
                     /translation="MTSTVTLRDSRFAQVVGSAVEFERIATGCLFTEGPLWHPGGQYL
                     LWSDMPGDHLRRWSAQDGVTTFRKPCNKSNGLAWDRQGRLLACEHASSQVSRTEPDGR
                     ITPLATHYQGKQLNSPNDIVCAADGGIYFSDPPYGRAEFYGVPRPQELAFQGLFRVGP
                     DRKSPVLLADDFDRPNGLCFSLDGRRLFVNDTARQHIRVFDVKGDGALSGGKVWAETT
                     GEGKGAPDGMKIDSRGHVYCCGPGGIHVFSSEAVCLGVIHVPEYVANFAWGDADYRSL
                     YITASTSLYRIRVQTPGLPAL"
     gene            16634..17881
                     /locus_tag="VFX87_11450"
     CDS             16634..17881
                     /locus_tag="VFX87_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995582.1"
                     /translation="MRTWIKNALLIDGTGAPARDGDVLIEGETIVHAGPLSTADAPRG
                     ADTQVVDVGGRTVIPGLIEAHIHLSYNNVKVIADLDLNCPPEYSTLVSAKNAELALSH
                     GYTAARSAGSVHGVDVALKRAINEGLYPGPRLLAASRDIVATGGMSDWNPSYLKLGMD
                     GLALIADGADQIRIAVRRSIKDGADVIKCYVGGDALLPHTPIADCTYTLDEVRVLVEE
                     THMRGRIAAAHVRGARSSRVAAQAGVDSIEHATYADDETLRLIADQGLVLVPGLRYLY
                     SIVENGPRFGITEEIVGPSGLRDEIKQAGDTYRRARDLGIRMCPGGDFGFAWNPHGEY
                     AKDIEVFVDVIGFTPLEAITCATRYGAELMRMQDRIGTLETGKLADLVVVDGDPLKDI
                     AVLQNRSRLSVMKGGRFVTRNLS"
     gene            17898..>18196
                     /locus_tag="VFX87_11455"
     CDS             17898..>18196
                     /locus_tag="VFX87_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995583.1"
                     /translation="MMLRVLTMLLALVLAMPLAASAQKPIKVGVPLPLSGPPALFGEP
                     ASKGALMFVEELNAKGGVLGRKIELVIRDSKADANEAVRVAREMILKDNVDFLMGT"
BASE COUNT         2662 a         6615 c         6292 g         2627 t
ORIGIN      
        1 cggagaatcc ccaggtgatc gaggcgtacc tgggcgagaa ggtggtgctg agctgatgct
       61 cgagctctcc caggtcaccg cgggctacgg cgccttcacc gcgctctggg acgtgagcct
      121 gcgcgtgaac cagggcgagg cggtggcggt ggtgggcccg aacggcgcgg gcaagaccac
      181 cctgctgcgc gccatctccg gtctcgtcgc gccgcgcgcg ggccgcgtca ccttcgaggg
      241 cgccgagctg gccgggcacc ccgcctacga catcgtggcg cacggcatcg cgcacgtgcc
      301 ggaagggcgc cggctcttcc cccagctcac cgtggccgag aacctcaaga tgggcgcctt
      361 cctctccgcc gcgcgcgccc acttccacga gagcctcgag cgcgtctaca cgctcttccc
      421 ggtgctcgcc gagcggtcca cccagcgggc gggcagcctc tcgggcggcg agcagcagat
      481 gctcgcggtg ggccgggccc tcatgtcgcg gcccaagctg atcctgttcg acgagccgtc
      541 catgggcctg gccccggtca tggtgctgcg cctgttcgat ctgatcagac gcgtgcgggc
      601 agatggatac accatcttgg tagtcgagca gaacgtgcgg caggtgctca agctcgtaga
      661 ccgcgcctat ctgctggagg tcgggcgcat caagatggag ggccgcgccg ccgacctggc
      721 cgagcaggac ttcgtgcgcc aggcctacgt gggcctctga gcatgatcga gccgatcttc
      781 atcctcgagg cggtggtgaa cggcatcctg ctcggcgggg tgctggccct cctggccctc
      841 gggctcaacc tgatcttcgg cgtcatcgac atcgtgtgga tcgcgtacgt ggacctcgtc
      901 gcgctctgca tgtacgcggt ctacttcctg gtcacgctct acggctggcc gctgtggctg
      961 gccgggctcg gggcgatcgc ggtcggcgcg gggctgggcg tcctcgtgca cctgctcatc
     1021 atcacgccca tcctgtcgag cccgcccatc aaccagcttc tcgccaccgg cggcctcctg
     1081 ttcttcatcc agtcggttgc cacgctcctc tggtccaccg atcaccgctc ggtgcgggtg
     1141 agcctgccca ccatcgaggt cggcggcatc ttcatctcct tctcgcggct gctcgcgttt
     1201 ggcctgtcca tcctggccat gatcgcgctc tacctcttcc tcaagcgcac ctacaccggc
     1261 accgcgatcc gcgcggtgag ccaggaccgc gaggccatcg cgctcatggg cgcgcggccg
     1321 cagcggctct acatcgtcac ctccgcggtg ggcggcgcga tggcgggggt ggcgggggcg
     1381 ctgctcatca tccagtacgc ggtgcatccc tacttcggcg gggccttcgg gccgctgacg
     1441 ttcatgatct gcgtgctggg cggcctcggc aacatggtgg gcgccttctt cgccgcgttc
     1501 atcatggccc aggtcatctc ggtgggcggg gtgatggtct cgaccgagat gggctacgtg
     1561 atcgccttcg tgatcttcat cgtgctgatg ttcgtccgac cgggcggcat cttcgccaag
     1621 cgcggatgac gatcgccttc cgcgtcctgg gcctggtcgc cctggtggca atggcgctga
     1681 tcccgttcct gcccctggga gccgcccagg agtacatctg gcacgtcatc atccagatct
     1741 tcctgtggtc gttcgtgggc aacgcgtggt ccctgatggg ccgcttcggc ctggtctcgc
     1801 tcggccacgg cgcgttcctc ggcgtggggg cctacgcgac cgtgctgctc tggaacttct
     1861 acgggatgac gccctgggcc ggcgtcgtcc tcgccgttct gctcaccgtc gccctggccc
     1921 tcgtgatcgc ctatccctgc tcgcgcttcc aggtggtcgg ccactacttc gccctcgtca
     1981 cgctcgcggt gggcgaggtg gtgcgcctgt ggatcgtggc cgagcgcgac tggacgggcg
     2041 gctccctcgg cgtgagcctc aagacgggcg gcaccgcctc gggcctgtgg acgctccagt
     2101 tcgcggacaa gcgcgtcttc tactatgtcg gcatggcggc ctggctcttc ggcctctggg
     2161 tgtggtggcg ggtggaccgc agcatggccc gctcggccat ggaggcgatc ggggaggacg
     2221 agaccgcggc ggcctcggtg ggcatccgcg tcacccggtt caagatgggc atcaccgcgc
     2281 tctcggccgc gctcaccgcg ctgggcggcg ccctgctcgc gctctacctc gcctacacga
     2341 acccggacac gctttcgggc atcggggtgt cgctgcgcat cgtcttcgcg gtggtgctgg
     2401 gcgggatgta ctcgctgctc gggcccttcg tgggcaccgc gctcaccatc gcgctcagcg
     2461 agtacctgcg catctacttc ggcatcaagc tcatcggcat cgcggagacg atctacggcc
     2521 tcttgctgat catcttcatc atcttcctgc ccgccggcat ctacggctcc atccgcgacg
     2581 cgctgttccg ccgtcgcccc gccggatcgg ccggcgccgc agtccgggcg ccctcgtgac
     2641 ccggctctcg ctcggcgccg gcccggcggc tctcaccctg gccacgatca tcgcggccgg
     2701 ctgtggcggc aaccccgccc tcgtcgtgcg cggcgggcag tcggaggctc agatcgcaca
     2761 ggaccgcgcc cagtgcctgt ccttcgtgca ggcgcacacc gagacgaccc ccgagctcgc
     2821 cgaggccgcg tgcctcgtca accgcggcta ccgcgcgccc ctcccgctcg cccaggggcc
     2881 ggcgggcatc ggctcgctct acgccaccgc caaccgggac gccaacgtga tcgtggcgga
     2941 cttccagggc tgccgcgtcg aggccttcaa caccccgatg cccgagaaca aggagaagaa
     3001 agccccctcg ggcatcttct cgagtttttt cggccagctc ttcccgcgcg gattcatcac
     3061 gaaggcccgc acgccggacg agtgggcgct ccagacgttc gcggcgtgcc tgaaccgccg
     3121 cggctacacc gtctcggacg tcacgcccgc ccgctagcat ccccgagcgg ccgcctggcc
     3181 gctgacgtag gcggcgcccc gacctcagcc gggcggaggc tctcggtgac gcacgaatcc
     3241 ggtcactacg accgcggcca cgcacacaaa gggcagccag gcgagcagca tcgacgatcc
     3301 cgcgtggagc acggtaccga ccagggcgcc cgccccgtag acgaggaaga ccgcggccag
     3361 gagtcgcagg tgccgcaagg cggcgccgga ggtctcgccg gatgacggcg cgcctcgtgg
     3421 ccgccgccag ctcacgagct ccgccatcag gctggtcagc gtgccggtga tgtacgtgga
     3481 cgccaccccg ggcacgccga gatggcgtac cgcggcggcc tggaccccca tggcgagccc
     3541 cgacagcgcg atgaggacca ggaccacgcc gtcggagcgc ggagccggcc cctccacgta
     3601 ccagatcacc ccaaagacgc ccagcaccac gccctcgagc gccagcgtgg cggtgacgat
     3661 gggaggccac tcgcctcgcc tttccccgcg cagcaggacc atcgcaccgc ccgccgcgcc
     3721 ggcgctgaag gcggccagcg ccagggtcga cctcaacgcg gccatgacct caccctgacc
     3781 gatggcgagc gccagcagca ccgtgttccc ggtcatcatc gcggtgaaga cgtgccccag
     3841 ccccagatag ctgatcgcgt cgacgagccc ggcggcgcag gtgaggacca ggagcatggc
     3901 gtcgcggctt ccgaccgcgc cggggatcgg ctcgctcggc tccccttcgc cgtcattcac
     3961 cggcatacgc ggatgggcca gtcattgatg ccactgtacc ctctcgggcg cgacgtcgcc
     4021 tcgtcgcctt ccggagcctt cgcggttcag gcataatccc cggcatggcg aggtggcggg
     4081 tggtgccgga cgaggagccg gcccgggcgg tggcggcgct ggccgaccag gtcgagcgcg
     4141 acggcggctc ggtgctcgcc atctatcgcg agccggtggg cgatcactgg cagatcttct
     4201 gcctcctgcc gctggagaag gtggagccca caccctatca gcgcgatctg tcgcccaccc
     4261 acgtcaagcg cctccaggag gtggtcaaga agatcgaccg cttcgtggac ccggtggtgg
     4321 tcatgtcgcc gcggcccggg gtctactgga cgcccaacgg caaccaccgg cgcacggtgc
     4381 tcatcaagct caaggccaag gtcatcccgg ccatcctggt ccccgagccc gaggtcgcct
     4441 tccagatcct ggcgctcaac accgagaagg cccacaacct caaggagaag tccctcgagg
     4501 tgattcgcat gtaccgcggc ctcgtggacg aggagccgca gtcgggcgag gaggactaca
     4561 ccttccagtt cgagtcggcc cacttcgtca cgctcgggct gctctacgag cagaacaagc
     4621 gcttcgcggg cggcgccttc gccccgatcc tgcggcgggt ggacaagttc ctgaaggggc
     4681 acttcccgaa gacgctcaag gagcgcgagg agcgggccgc gctcgtgcgc gacgccgacg
     4741 aggcgctcgt cgcggtggtc gcgcggatca agaagcgcgg catcaaccac ccctacgtga
     4801 agaacttcgt cctcgcgcgc accaccccgc tgacgcgcca gcggaagacg ctgcccggct
     4861 tcgagcagac cttcaagaag ctcgtcgaga acatcgaggc cttcgacgtc gccaaggtgc
     4921 gctacgagga catccagcgc tccgccatca tgggcgcgcc tgcagcaggc taggcgctcg
     4981 acgtggcccg gcccaccaag ctcgccgacg cggaggtgac gcagcgcctg gccgccctgc
     5041 ccgggtggcg gctcgagggc ggcaagctcc accgcgagct caccttcggg gacttcaccc
     5101 aggccttcgc cttcatgacc gacgtggccc gcgaggcgga cgcgctcgac caccatccgg
     5161 agtggttcaa cgtgtacaac cgcgtggtca tcgatctcgc cacccacgat gcctccggca
     5221 tcaccacgct cgacttcgag ctggcgcggc gggccgagga cctggccggc cgccgcgccc
     5281 gccccgcgcc atgaccggcc gcgtggcggt cgtcaccgga ggcaccggcg cgctcggcca
     5341 ggcgatcacc ccccacctgc tcgaggccgg cgggatcgtg gccattccct acgcggtgcc
     5401 ggaggagctc accgggctcg aggcgcggct ggcccccgag ctgcgcgcgc gtctcctcgc
     5461 cctgcccgcc gacgtgaccc gcgaggacgc gatgggcaag ttcgtccagg cggtgcgtga
     5521 ccggcacggc cgagtggacg gcctcgtcaa cgcggtgggc ggcttcgcgg gcggcgatct
     5581 gctcgcgacg tcgctggcgg agtgggaccg catgatggcg ctcaacctca cgtccaccgt
     5641 catcgcctgc cgcgcggtgc tgccggtcat gatcgcgggc ggcggcggtc gcgtcgtcaa
     5701 catcgcctcg cgcgcggtgc tctcaccgca gggcggcttc atcgcctaca ccgtctccaa
     5761 ggcgggcgtg atcacgctca cgcaggcgct ttcccaggag gtgaagaagc acggggtcac
     5821 cgtcaacgcg gtgctgccga gcacgatgga cacgccgggc aatcgccgcg ccatgcccga
     5881 cgcggatcgc tcggagtggg tcagcaccag ggacgtggcc cacgtggtgg ccttcctgct
     5941 ctccgagcgg gccgccgcgg tcaccggcgc atccctcacg gtctgaccgg ggccgcgcga
     6001 tggagacctt cgatctcgtc gtgatcggcg ccggcgccgc cggcctcacc gcggcccgga
     6061 ccgcggccgg cctgggcgcg cgggtggcgc tcgccgaccg cggcccgctc ggcgggctct
     6121 gcgtcaaccg cgggtgcatc ccgaagacgg cgctggtggc cgccgggcgc gtgcatcggc
     6181 aggtgcgcga ggcgggcggg ttcgggacct tctcgggccc ggtccggctc gactgggacg
     6241 ccgtgcagca acgccaggac gagatcgtga acgcgcatca cccctccgcc gccgatctcg
     6301 agaagcgcgg catccgggtg gcgatcggcg aggtccgctt caccgacccg cacatggtcg
     6361 aggtgaacgg ccggccgctc ggcgcggagc gcttcgttat cgcggcgggc tcccagccgg
     6421 tcatccccga ggtgcccggc cgcgagctgc tcatcacctc cgaccagctc ctcttcctga
     6481 ccgaattccc ggcctcgctc accttcatcg gcgcggggcc ggtggcgctg gagctggcgg
     6541 gggccttcaa cgatttcggc tcccgcgtca ccgtgctggc gcgcgacgcg gagattctcc
     6601 ccgggctcga cgcggacgtg gcccggaacc tgcgcaagca catggaggag cgcggggtga
     6661 cgttccggct gcagaccagg gtgaccggcc tcgcccgggc gacggagggc gtgcgcatcg
     6721 ccttcctgag cgccggcatg gcgaacgaga tgacgagccg gcaggtatgc gccgcggtcg
     6781 ggcgccgctt ccacccgcgg accatcgggg cggcccgcat cggcctcgag atgggacggc
     6841 tcggcctgcg cacgaatgcg cacctcgcga cctcggtccc gcacatctac gcggcgggcg
     6901 acgcggcggg caaccggcag ctcagcacgg tggccgccta cgaggggcgc atcgccgcgc
     6961 tcaacgccct gcggggcgat accgagcggg ccgacgaggc ggtgatcccc caggtgctct
     7021 ccacgacccc cacggtcggc ctcgtgggcg tggcctacgg cgaagcaccc gcgcacggca
     7081 tccatgccgc ggtggcccgc cacgacgcgc tgggcagcag ctacagcgtc gtcaccggcg
     7141 aggacgcggg ctacttcaag ctcgtcttcg acgaggactc ccagcggctc gtcggggccc
     7201 agatggtctc gccggccgcg gaggagctga tccagctctg cgcgctcgcc atccgcgcgc
     7261 gggtgcccgc gtccctggtg gagacgcagc tctcggtgca ccccagtcgg accgaccacc
     7321 tgatgcgggc cttcgcgccc gagccgggcg cagtcccccg ccccgtcgag ggcgccgacg
     7381 tagtagaatc gcctccgtga tccgacccgc ttcgcggcgc gcggtgccgt ggctggccgt
     7441 cgccctgctc gtctctgcct gcgccgggtc cggtgcgcgc tccgcgccgc ccgccccggg
     7501 cgccgccgag gccgagctcc ccccgccggt gcgccaggtg ctgcgcaacg gcatgcgcct
     7561 catcgtgcag gaccaccggg ccgccgacat cgtggcggtc tacctcttcg tcggggtcgg
     7621 ggtgcgctac gaggcgcccg accagctcgg ctacgcccac ttccaggagc acatgctctt
     7681 caagggcacc gaccgcttcg gccccggcta cgtggaccgg accgtggaag gtgtgggcgg
     7741 ccgctcgaac gcggtgacct cgttcgacta cacggagttc catctggtgg tgccctccga
     7801 ggcgaccgcg accggcatcg agctgcttgc cgacatggcc ttccgctcca gcttcgatcc
     7861 cgacgaggtc gggcgggagc gcgaggtcat cttcgaggag gcgcgcatcg agcaggacaa
     7921 cccgcgctcg gcggtggtgc gccagctcta cggcctggtc ttcgcgggca atccctacgg
     7981 gcgtcccgtg ctgggcaccc gtaccacgat gcaggcggcc acccgggagc ggctgcgcgc
     8041 cttcaaccgc cgctactacg tgccggagaa catgacgctg gtggtggcgg gaccggtgga
     8101 tccggtcgcg gtgcgcggca tcgtggaccg cacgttcggg cgcatgccgg ccacctccta
     8161 caagcccacc ccggcgcccg ctccggcgcc gctcaccggc ggggtgcgcc gcaccgtgga
     8221 gcgcgacgag cagcaggctc acctggccct cggctggcag gccccgcgct cggacgacgc
     8281 caacggcgac gcggtggacc tcctcaccac gatcctcgcg ggcaccgaga gctcgcgcct
     8341 ggcccagcgg ttgcgcgacc gggagcggct ggtctccacc gtgaccatga gctacgcggc
     8401 gctcatgggc ggcggcatcg tgagcctgcg ggcggagctc gaggccaagg atctggagcg
     8461 ggtggagcgc atcatcctgg aggagatcgc gcggatgcag gagagcgggc cgaccgagga
     8521 ggagcgccgc ctcgcggtca ccaagttcga ggcgcagcac gcgttcgaca ccgagacctc
     8581 cgagggcctg gcctacgcct acggcctcgc cgagaccacc tggacgctcg acgccgagct
     8641 gcgctacgtg gaccggctcc gccgcatcac ccgcgagcag atccgggacg cggcgcgccg
     8701 gtacctctcg cgcaccgact acgcacggct ggccttcgtg ccgaagcccg ggactcccag
     8761 caccagatga tgcgcggacg cgcgctcctg gtggccggcc tcctcgcgct gctcgcggcg
     8821 cccgcgtggg cccagagccc cgtggtcagc cgggcgcggc tccccaacgg gctcaccgtg
     8881 ctggtgcgcg agaatccgac cgccccggtc gtgggcatct ccctgatggt ccgcatggga
     8941 acacgctggg agacggccga caacgcgggc atctccaacc tggtccagct catgatcgtc
     9001 cgcggcacca ccacccgcga cggcggccag atcgtcgagg cggccgacgc gatgggcggc
     9061 agcatcgagg ccaccggcga cgtggactac tcggaggtcg cggccaccgc gctctcgcgc
     9121 cactgggtgg agatgctcga gctggtcgcc gacgtggccc tccggcccac catgccggac
     9181 ggggtggtgg tggcggtgcg cgatttcctc ctccgccagg tgcgcaatcg tggcgacaag
     9241 ccctacgacg tggcgctcga tgcgctcacc gcgcgcgtct tcggccgcca cccctatgcc
     9301 tgggatccgc tcgggctcaa ggagagcctc gagcggctcg atcgcggcgc gctccttgcc
     9361 cactacaagc ggcactacgt gccgggcggc ctcgtcctgg cggtgagcgg ccgcgtcaag
     9421 acggccgagg tgatggcgca ggccacgcgg ctcttcgggc cgatgcccgc gggggccgcg
     9481 ccggccgcgg ccccgccacc gccccccgca ccggccccgt ggcgcgaggc gcgggtggtg
     9541 cccggcgcgc aggcgcagat cctgatgggc gcgctcgcgc ccgcgctcac cgacccggat
     9601 tatccggcgg tcaaggtgct ggccacggtg ctgggcggcg gaatggcggg acggttcttc
     9661 tcggagctac gggacaagca gggcctcgcc tacacgacgg cggcgcagta cccggcgcgc
     9721 gtggacacca gcttcttcct ggtccagctc ggcaccgcgc cggagaacgt ggcgcgggcg
     9781 gaggcggcgc tgcgggcgca gctcgagcgg gtgcagcgcg agcgcgtgtc gaacgaggag
     9841 ctgcgggtgg ccaaggcgta cgtgctgggg aacctcgcga tggaccggcg caccaacgcg
     9901 cgccaggcgt ggtacctggc ggcctacgag gaatccgggg tgggctacga gttcctggac
     9961 cgctacatga cggcggtgcg cgcggtgacc gccgccgacg tgcagcgcgt ggcccggcgc
    10021 tacctggccg tcctgcgcac cgtcatcgtc cagcccaccg cgccctgagg cggtcgcctc
    10081 agcgcatgcg ggtcatctcg atccgcaccg gcgagctggt gtacaggaac tggccgacga
    10141 gcttgtcccc ctgcagcgtg aacgtggccg cgaacggccg gcccggctcg gtgccgttca
    10201 cccacatctc ggtctccgag acggacagct cgatgggcga gccgaacgac gagttctcgc
    10261 ggagcgatag cgggacgccc tcggccatgc gcgcgtcaac gagcacgagc cggccctggc
    10321 cggtggcgcc gtcgaccgtc aggaccgcct gcgccacccc gccccgcagc gtgcgggaca
    10381 tgccgtaacc gctccaggtc ccctcccagc gcccggcaac atcggcccgc ggcatggcga
    10441 atggggtgtt ccgtccccac cacccctcgc ccttggcctg atccatcagc tccggcgtga
    10501 cctggggcgc ggcgcccacc gtgttcttca tcgagaaggc cagatgattg gtggtggcgt
    10561 agtgcgtcgg gtgcgcccgc cagacctccc actcgcggtg agtggcagcg cagccggtgg
    10621 acaggattac cgagcccagg aggagcggga aaaagatccg gtggcgcatt ggtgagtccc
    10681 tccaatcaaa atccatgtgg aatcacagtg ttgcacaccg gaattttcca cccaagctat
    10741 tcgagcagct ccggaaacgc gcggatgatg cggtgcggcg ccaccgagca ggacttcggc
    10801 agcccctggc ccggggcgtc cacccagaca ccccggagcc ctaggcgctg gggcgccagc
    10861 acgtcccatt cgaggttgtc ccccaccatc caggcctcct ccgccttcac cccgagcttg
    10921 gagagggcat agcggtagac gacctcctcg ggcttgccga cccccatctc gccctccacg
    10981 acgatcacgt cgaagaaccg ctcgagatcg tgccgctcga tcttgcgccg ctgatggctg
    11041 gcgtccccgt tggtgaccat ggccagcggg acgccccggg cgcgcagggc ctcgagcgcg
    11101 ggggtgacgc ccgggaacag cgtcatgcgc tcccagcggc gctcggcgaa gtcctccgcg
    11161 atgcaggccg ccagtgcctc gtccgcgcgc cccaggcggc cgagcgcgtg ggcggtgatc
    11221 cgggtccagg cgcggagcat gtcggcgcgc tccgtgcggt ggcggctcgg gtcgctccag
    11281 aaccagcgcc tggtctcggc gatcgcggcc acgaggccgg gcgggtcgat gccggcgggg
    11341 ccggcgagcg cctcgcaggc gtgcagccag cactcgtcca tgccgcccga gtaatcgagc
    11401 agcgtgtcgt cgagatcgat cagcagggct ctcaggacgt gacctcctgg ggagcggtga
    11461 agggcgcgag ctcggggcgg cagaccgccc ggaaattgca ccagcggcag atgcgcagct
    11521 cctcggtctt ctcgaagccc gactcctcgg cgagattgcg ctcgggatcc ttgaggtagg
    11581 ccttcatgga gcggaccgag agccggatgt gctcgcggac ccggtcgagg ctctcctcgt
    11641 cccacgggtg cgtcgtcacc ttgccctcgc ggaggttgac ctccagcagg tccacgcgac
    11701 cgagatccac gcccagcacc tcgagggcgt agagggcgta gccgccgagc tgcagcccgg
    11761 cgccctcgcc gccgccgccg gtcttccagt ccaggagctg caaccggtcc gcgtggctcc
    11821 agtagccgaa gtcgggcgca ctgaacacgc gcgtgccctc gaaggggaag gagccgatgt
    11881 cctcgatgaa gacccagcgc tccgtcggcg tgcgcttgat gtcggtcagc acgggcagcc
    11941 ggtggaagtt ccgcaggcag cggaccacgt gatcgcgcag ggcctgccac tcgctatcgc
    12001 ggatggccac gccgtactcg tgctcgaaca gggagggccg cttcggggcc tgccggtaga
    12061 cccccgcacg cgagcccttc cactcctcgc gcatctggcg caccgtatcc tcgatgaggg
    12121 aggcctcgct caggccgtgg ccgtcgcgca gggccaggag ggagcgctcg atcgcctcgt
    12181 ggacgagccg gcccgcccac atctggcggg tcgcgagctg cttgaggacg tagaggcggc
    12241 ggacgagcgg gtcggagtcg gcgtcccagc cgccccaggc gccgtagtac tgatagtagt
    12301 agcgccggcg gcagtcctgg aagacattgt cgcgggtgcg cgaccaggag aattcgttca
    12361 cgaggtcggc cacgtgtaga gcatagcatt ccgggcgcga cacccgagcc cgggcgcttt
    12421 gcccgctccc ccggccggcg ccctgccgcc ccctcattcg gaatcgagaa cttgcagagc
    12481 cacccttcgt ggcacacttg gtgcgttcgc gtccgcgaga gcttcccaca ggagggatcg
    12541 ccatgagaat catcgccgga gcactgatca tcgtcgggct cgccaccctc accgccggcc
    12601 ccatccgggc ccagggcaag gccaccgagc gcctcgggac ggtccggttc gtgacgtcct
    12661 gcgcgccggc cgtccagccc cggttcgatc gcgcggtggc gctgctccac tcatttcacc
    12721 tggacgccgc ggtgggggct ttcacggagg tggcgaccgc ggaccccgcc tgtgggatgg
    12781 cgcactgggg cgtggccatg gcgtggctgg gcaacccgct ggcgggcccc ccgagcgccc
    12841 gcggcctgaa agagggctcg gccgcggtcg accgggcgaa ggcggcggga gccaagaccg
    12901 accgcgagcg tgagtacatc gccgcgatcg agacgttcta caaggacgcg gaccgggtgg
    12961 accaccgcac ccgggcggtg gcctacgagc gcgccatgga gcagatcgcc gcgcgccatc
    13021 ccgccgaccg cgaggccgcc gtcttctacg cgctcgcgct caacatcacg ctggatccca
    13081 acgacaagac ctacgccaac cagctcaagg ccgccgggat cctcgaaaag gtcttcgccg
    13141 agcagcccga ccatcccggc gtggcccact acctgatcca cagctacgac ttcccgccga
    13201 tcgcggccaa gggcctgccc gccgcgaagc gctacgccgg catcgccccg gcggcgccgc
    13261 acgcgctgca catgccgtcg cacatcttca cccgcgtggg caactggcag gagtccatcg
    13321 agaccaatcg cgcgtccgcc aaggccgccg cggacacgct ggccgccggc gggtcgcaga
    13381 ccggcctgcg ctcgtacgag ggcctgcacg cctacgacta catgatgtac gggtacctgc
    13441 agctcgccag ggaccgcgag gcgcgggccc tggtggaaga ggtcaccgcg acccggaagc
    13501 tgaacgtgga ccacttcgcg ggcgcgttcg ccctcgccgc catcccggcg cgctacgcgc
    13561 tcgagcgtgg ccagtgggcg gacgcggcca cgctgacccc gaccccatcg gacctcgcct
    13621 ggaatcgctt cccgcaggcc gagtccatcg tctggttcgc gcgcggtctc ggcgccgcgc
    13681 gcagcggcaa cccggtgcgc gcccgggagg acctcgcccg gatcgaggct ctccgggact
    13741 cgatgaccgc ggccaagagt ctctactggg ctcatcagag cgaggtgcag cggctggcgg
    13801 tcgcggcctg gatcgcccgc gccgacgggc ggaacgagga ggccctgtcg ctcatgcggg
    13861 cctcggcgga cgccgaggac gccaccgaga agcacccggt gacgccgggt tcgatcaagc
    13921 ccgcccgcga gctgctcggg gagatgctga tcgacctcgg gcagccggcg gaggcgctca
    13981 agcagttcga ggcctcgcat cgggtggagc cgaatcgctt ccaggggctg tacggcgccg
    14041 cccgcgcggc cgagctggcc ggcgaccgcg ccaaggcccg cacctactac gggcggctcg
    14101 tggccctcgg ccagggggcc gatggcgacc gaccggagct caagcaggcg aagatcttca
    14161 tcgctacgaa atagcctcga cgcgatcggc ccggggaacc cggacgaccc ccgcgcagtt
    14221 tccgtgcgcg ggggtctttc tttcgacatc gcccgctcac ccagtgtcca cattcgagcg
    14281 cttctgctag agtgtggcaa ccctgacgca gctcacgctg gtgaagagga ggctctgaca
    14341 tgccactcgc tccgcgatac ctggcggccc tggtccccgt ggtgctcctc gcgctcgtgc
    14401 tggcgacatt cggctcgctc gacgcccagg cgggcaagat cgaggtgctg tggctgggcc
    14461 agtcggccac ccgcatcacc accgtgaccg gcaaggtgat cgtgatcgat ccgttcctca
    14521 cccagaaccc caagacgccg gcgcagtaca agaacctgga cgccctgggc aaggtcgatc
    14581 tcatcctggt gacgcacgcc cacggtgatc acctgggcga cggccccgag ctggccaaga
    14641 agcacaaggc gccactgtgg gggccggcgg ggctggacca gtcgctgctc acgctcggcg
    14701 tgctgccggc cgagctggcg ccgcggatga acaagggcgg cacgatcacg ccgctcggac
    14761 ccgacatcaa gatcacgatg acgcgggccg agcacagctc ggagctcgtg tggaccaacc
    14821 cggccaccgg caagccggag acgcacgtgg gcggcgagcc ggccgggttc ctgatcgagt
    14881 tcgagaacgg gttcaagctc taccacatgg gtgacaccgg ggtcttcgcg gacatgaagt
    14941 ggatcggcga gtactacaag ccggacgtca tcatgatccc gatcggcggc cacttcgtga
    15001 tgagcccgaa ggacgcggcg gtcgccaccg aaatgatcaa gccaaagttc gcgatcccca
    15061 tccactacgg caccttccca ccgctgaagg gcacgccgca ggagtacatg caggcgctcg
    15121 gcgccaccgc ggtcaaggtg ttcccgatca accccggcga caagctgatg ttcccgtgac
    15181 gcgggcgggc ggctgcgcga gaacggcttc agccccggcg acacgctgac cttctagcgg
    15241 cggaccgggc cccgccgggc ggtccggcgg agcacggtga ctgacgcgcc gcgctcttgc
    15301 tccctcggcg tccggcgtgc taggctcccg gcattttccg gggggcaacg tagcgctccc
    15361 catcacgctg gagccaaggg agactccgat gccattcgtg agcattcgca tctacgaggg
    15421 ctggggcaag gagcgcctga acgagatcgc caagcgggtc accgccgcga tcaccgacgt
    15481 cacccagcta cccaaggaag ccgtctgggt ggtcttcgag gaagtcgacc cgcccgactg
    15541 gtacgtcggc ggcaaggccg gcgagcgcat ggccaagaag tgaccacggt cgagccgcag
    15601 gtgggggggc ttgggggagg agcggcgccg cccctctcaa ccacaagatg acctccaccg
    15661 tgacgctccg ggactcccgc ttcgcccagg tcgtgggctc cgcggtcgag ttcgagcgca
    15721 tcgccaccgg gtgcctgttc accgaggggc cgctctggca tcccggcggc cagtacctgc
    15781 tctggagcga catgccgggc gatcacctcc gccggtggtc cgctcaggac ggcgtcacga
    15841 cgttccgcaa gccctgcaac aagtccaacg ggctcgcctg ggatcggcag gggcgcctgc
    15901 tggcctgcga gcacgcgagc agccaggtct cccggaccga gccggatggc cggatcacgc
    15961 cgctggccac ccactaccag ggcaagcagc tcaacagccc caacgacatc gtctgcgcgg
    16021 cggacggggg catctacttc agcgacccgc catacgggcg cgccgagttc tacggcgtgc
    16081 cccggccgca ggagctcgcg ttccagggcc tcttccgggt gggcccggac cggaagagcc
    16141 cggtgctgct cgccgacgac ttcgaccgac ccaacgggct ctgcttctcg ctcgacggcc
    16201 gccggctctt cgtcaacgac acggcccgcc agcacatccg cgtgttcgac gtgaagggcg
    16261 acggcgcgct ctccggcggc aaggtgtggg cggagaccac cggggagggc aagggcgcgc
    16321 ccgacgggat gaagatcgac tcccggggcc acgtgtactg ctgcggcccg gggggcattc
    16381 acgtgttcag ctccgaggcg gtctgcctgg gcgtcatcca cgtgccggag tacgtcgcca
    16441 acttcgcgtg gggggacgcg gactaccgga gcctctacat caccgcgtcc acctccctct
    16501 accgcatccg ggtccagacc ccaggcctgc ccgcgctatg agaaagacgc gaagtcacct
    16561 cgcagaggtc gtcgcacgcg acgaccgtag aatcaaatga ggcgtgttgc cccaccgcag
    16621 cggacagccc accatgagaa cctggattaa aaacgccctc ctgatcgacg gcaccggcgc
    16681 gcccgcgcgc gatggcgacg tgctcatcga gggcgagacc atcgtgcacg cggggccgct
    16741 gtcgacggcc gacgcgccgc gcggggccga tacgcaggtc gtcgacgtcg gcgggcgcac
    16801 cgtgatcccc ggcctgatcg aggcccacat ccacctctcg tacaacaacg tgaaggtgat
    16861 cgcggatctc gacctgaact gcccgcccga gtactccacc ctggtgtcgg ccaagaacgc
    16921 ggagctggcg ctctcgcacg gctacaccgc ggcgcgctcc gccggctcgg tgcacggggt
    16981 ggacgtggcg ctcaagcgcg cgatcaacga gggcctctac ccgggccccc ggctgctcgc
    17041 ggcgagccgc gacatcgtgg ccaccggcgg catgtcggac tggaacccgt cctacctcaa
    17101 gctcggcatg gacgggctcg ccctcatcgc ggacggggcg gaccagatcc ggatcgcggt
    17161 acggcgctcg atcaaggacg gcgcggacgt gatcaagtgc tacgtgggcg gcgacgcgct
    17221 gctgccccac accccgatcg cggactgcac ctataccctg gacgaggtcc gcgtgctggt
    17281 ggaggagacc cacatgcgcg gccgcatcgc ggcggcccac gtgcgcggcg cccgctcgag
    17341 ccgcgtggcc gcccaggccg gggtggactc catcgagcac gccacctacg ccgacgacga
    17401 gacgctgcgc ctgatcgccg accagggcct ggtcctggtg ccggggctgc gctacctcta
    17461 ctcgatcgtc gagaacgggc cgcgcttcgg catcaccgag gagatcgtgg ggccgtcggg
    17521 gctgcgggac gagatcaagc aggcggggga cacctaccgc cgcgcccgcg acctgggcat
    17581 ccgcatgtgc ccgggcggcg acttcggctt cgcctggaat ccgcacggcg agtacgcgaa
    17641 ggacatcgag gtcttcgtgg acgtcatcgg gttcaccccg ctcgaggcca tcacgtgcgc
    17701 cacgcggtac ggcgccgagc tgatgcggat gcaggaccgg atcggcacgc tcgagaccgg
    17761 caagctggcc gacctcgtgg tggtggacgg cgatccgctg aaggacatcg cggtgttgca
    17821 gaaccggagc cgcctgtcgg tgatgaaggg cggccggttc gtgacgcgca atctctcgta
    17881 gccaaaggag gcttcccatg atgcttcgcg tactcacgat gctgctggcg ctggtgctgg
    17941 cgatgccgct ggccgccagc gcccagaagc cgatcaaggt cggcgtgccc ctgcccctct
    18001 cgggcccgcc cgccctgttc ggcgagcccg cctccaaggg cgccctgatg ttcgtcgagg
    18061 agctgaacgc caagggcggc gtgctcggcc gcaagatcga gctggtcatc cgcgattcca
    18121 aggcggacgc caacgaagcg gtgcgggtcg cccgcgagat gatcctgaag gacaacgtgg
    18181 acttcctgat gggcac
//
LOCUS       DASUFA010000306         5251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1319242, whole genome shotgun sequence.
ACCESSION   DASUFA010000306 DASUFA010000000
VERSION     DASUFA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319242"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFX87_11460"
     CDS             complement(<1..188)
                     /locus_tag="VFX87_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX4995584.1"
                     /translation="MLKRFGLAVMTLALVAALAGGAAAQQSLTWTAGAVGGGWYAISG
                     GMAELLREKAGLNIKVVPG"
     gene            complement(214..1371)
                     /locus_tag="VFX87_11465"
     CDS             complement(214..1371)
                     /locus_tag="VFX87_11465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4995585.1"
                     /translation="MTQTADVVIIGAGIIGGSIAFHLLERDPRLRVVILEKEAEPGTG
                     ATSKATGGVRFQFSTEANVRLTQLSYRYFTHDREILGCSVDFLRHGYLFVTTDAATFE
                     QCRQNVALQRGLGVASELLTPAQIQTHLAPLVTGDLVGGTFCAEDGSADPYSLLAGFL
                     AVARSRGLRLLTGQPVTAIVKSGTRGTGVKTPSEEWSAPVVIDCAGPHADEIGAMAGV
                     DIPSKPYRRQVMVTEPLPVLPDVFPLIVDLDTGFYVHRQGLSAVLMGGTDKDIKPGHD
                     TTVDWDAFDAVFRAAALRVPPLALAQVMRAYAGVRDLTPDYHGILGPAPQAPGFHVAC
                     GFSGHGFMHSPAIGILMTEMLLDGHARSMDIAALSLDRFAAGRLRAEANMF"
     gene            complement(1385..2113)
                     /locus_tag="VFX87_11470"
     CDS             complement(1385..2113)
                     /locus_tag="VFX87_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX4995586.1"
                     /translation="MRVRGGQNLYGFSVGILMLDTQFPRIPGDMGNASTFDFPVRYHR
                     VAGASPDRVVRQGQRELLPSFIEGARFLEREGVRAVTTNCGFLAKFQPELAAAVSIPV
                     FTSSLMLVPLVHRMLPARRPVGILTVNASALHAEHMAGAGIAPDVPVVVAGLETEKEF
                     TRVMLDDQLTLDVETARQEHLTVVRRMLDAHPEIGAIVLECTNMPPYRADIEALTGLP
                     VFDITTLVRMVHDAVRHGLPPRPA"
     gene            complement(2124..2957)
                     /locus_tag="VFX87_11475"
     CDS             complement(2124..2957)
                     /locus_tag="VFX87_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX4995587.1"
                     /translation="MDADPSLAAFAVAVDRPDDQIHLARAALLIAAGEQPDLDMDDYL
                     GRLATLAEAAEPARRADDALGRLHRLREYLFEELGFSGDRDDYFDPRNSHLNEVLNRR
                     LGIPITLSLVLIEVGRRLDLEMEGIGLPGHFITGARIAGEQVLLDPFNRGALLTTESC
                     RELVSRALGRPVRLAPEHFVPVGNRQFLARMLANLKGVYWRREAWDKVVRVIDRLLVL
                     NPASAGERRDRGAAWSNMGRLERGVADWEQYLTDFPNAADHEQVRNLLRRVRQKLAQL
                     N"
     gene            complement(2957..3616)
                     /locus_tag="VFX87_11480"
     CDS             complement(2957..3616)
                     /locus_tag="VFX87_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX4995588.1"
                     /translation="MTAAEFVAQLDEANQAVLRRLEPEATLKPEVSGNLNVMNLLKVA
                     LKNEIEATEIAARWLVATTDVEVKMAFARQAGDEAKHYRMIAERLGEMGFDARAFDPL
                     AQGFGPLFKYLDSLETTVERVAAGQFTREAIAVVKNRQFIEFCDRAGDRLTATLYREI
                     IEPDERFHHQLGRSLLLKLATTPETQEAARRASARTLALAEELQGAALRTAGIHHAPG
                     C"
     gene            complement(3613..3816)
                     /locus_tag="VFX87_11485"
     CDS             complement(3613..3816)
                     /locus_tag="VFX87_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995589.1"
                     /translation="MDLTTSGWDYEAMFTALERVPAAPVLGYTTHALARTTQPLHARC
                     RRVVTKETLTQELPELLQRGLAA"
     gene            complement(3977..4525)
                     /gene="def"
                     /locus_tag="VFX87_11490"
     CDS             complement(3977..4525)
                     /gene="def"
                     /locus_tag="VFX87_11490"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX4995590.1"
                     /translation="MAILKVARLGHPVLRKVAAPVPVSEILSAETQRLIDDMVETMRE
                     YNGAGLAAPQVHALKQICVIEVHGNPRYPDAPAIPLTVLINPVVTPLTDETEDGWEGC
                     LSVPDMRGIVPRFGSARLEARDREGNPIDVVAKEFFARVIQHETDHLNGIVYVDRMRD
                     LSTLTHLAEWNRYWLGVQEQDE"
     gene            complement(4651..4875)
                     /locus_tag="VFX87_11495"
     CDS             complement(4651..4875)
                     /locus_tag="VFX87_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995591.1"
                     /translation="MKKQIVFYASTRTYQAVLAIHGREDLVPRLHAKSLAGDWEGMAD
                     LISDETLDHFAVSGSFATIGERIRERYRGL"
     gene            complement(5012..>5251)
                     /locus_tag="VFX87_11500"
     CDS             complement(5012..>5251)
                     /locus_tag="VFX87_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995592.1"
                     /translation="RMREVVQALRAIWDCWQRGTPLDFRRRSYHFDLMTPFFNPGPIE
                     HPLIPIYLAGVNPQMCRIAGEVADGLHIHSFHSAR"
BASE COUNT          781 a         1777 c         1911 g          782 t
ORIGIN      
        1 cccgggacga ccttgatatt gagccccgcc ttctcccgga gcagctcggc catgccgccc
       61 gagatggcgt accagccgcc gcccaccgcg ccggccgtcc acgtcagcga ctgttgcgcg
      121 gccgcgccgc cggcgagcgc cgcgaccagg gccagagtca tcaccgcgag cccgaaacgt
      181 ttcagcatcg gtcctgcctc ctgtctccgc tactcagaac atgttggctt cggcccgcag
      241 ccgtcccgcg gcgaagcggt cgagggataa tgcagcaata tccatgctgc gggcgtgccc
      301 gtcaaggagc atctcggtca tcaggatgcc gatggcgggc gagtgcatga agccgtgccc
      361 ggaaaaaccg caggccacgt ggaagccggg cgcctgcggg gcgggtccga ggatcccgtg
      421 gtagtcgggt gtgaggtcgc gcacgcccgc gtaggcgcgc atcacctggg ccagggccag
      481 gggcggcacc cgcagggccg ccgcgcggaa gaccgcgtcg aatgcgtccc agtccaccgt
      541 ggtgtcgtgc cccggcttga tgtccttgtc ggtgccgccc atgagcaccg cgctcaggcc
      601 ctggcggtgg acgtagaagc cggtgtcgag atcgacgatc aggggaaaga cgtccggcag
      661 caccggcagc ggctcggtga ccatcacctg gcgccggtag ggcttcgacg ggatgtccac
      721 gccggccatg gcgccgatct cgtccgcgtg gggcccggcg cagtcgatca cgaccggcgc
      781 ggaccattcc tcgctcggcg tcttcacacc ggtcccgcgc gtgccgctct tcacgatcgc
      841 ggtgacgggc tgtccggtca gcaggcgaag gccccggctc cgcgccaccg ccaggaagcc
      901 cgcgagcagc gagtacggat cggcggagcc gtcctccgcg cagaaggtgc ccccgaccag
      961 gtcgccggtg acgagcggcg ccaggtgcgt ctggatctgg gccggcgtga ggagctcgct
     1021 cgccacgccg agcccgcgct ggagcgccac gttctggcgg cactgctcga acgtggccgc
     1081 gtcggtggtc acgaagaggt agccgtggcg gaggaagtcc accgagcagc cgaggatctc
     1141 gcggtcgtgg gtgaagtagc ggtacgagag ctgcgtgagg cgcacgttcg cctcggtgga
     1201 gaactggaac cgcacgccgc cggtggcctt cgaggtggcc ccggtcccgg gctccgcctc
     1261 cttctccagg atgacgacgc gcaggcgcgg gtcgcgctcg aggagatgga aggcgataga
     1321 gccgccgatg atgccggcgc cgatgatgac gacgtcggcg gtctgggtca cgagcgcgac
     1381 gagatcaggc ggggcgcggc ggcagcccgt ggcgcaccgc gtcgtgcacc atgcgaacca
     1441 gcgtcgtgat gtcgaagacg ggcagaccgg tcagcgcctc gatgtcggcc cggtacggcg
     1501 gcatgttcgt gcactcgagc acgatcgcgc cgatctcggg atgggcatcg agcatgcgcc
     1561 gcaccaccgt gaggtgctcc tggcgcgccg tctccacgtc gagcgtgagc tgatcgtcga
     1621 gcatcacgcg ggtgaactcc ttttcggtct cgagtccggc caccaccacc gggacgtccg
     1681 gggcgatccc cgcccccgcc atgtgctcgg cgtgcagcgc ggaggcgttc acggtgagga
     1741 tgccgaccgg gcgccgcgcc ggcagcatgc ggtgcaccag cggcaccagc atgaggctcg
     1801 aggtgaacac cggaatggag accgcggcgg ccagctcggg ctggaacttg gcgaggaacc
     1861 cgcagttcgt ggtgaccgcg cgcaccccct cgcgctcgag gaagcgcgcg ccctcgatga
     1921 aggagggcag cagctcccgc tggccctggc gcaccacccg atccggggag gcgccggcca
     1981 cgcggtggta gcgcaccggg aagtcgaagg tggaggcatt gcccatgtcg cccgggatgc
     2041 gcgggaactg ggtgtcgagc atcaggatgc ccaccgagaa gccgtagagg ttctgcccgc
     2101 cccggacacg catgcgagga tgactagttg agctgcgcca gcttctggcg aacgcggcgg
     2161 agcaggttgc gcacctgctc gtgatccgcc gcgttgggga agtcggtcag gtactgctcc
     2221 cagtcggcca ccccacgctc gagccgtccc atgttgctcc acgccgcgcc gcggtcgcgc
     2281 cgctcccccg ccgaggcggg attgagcacg agcagccggt cgatcacccg caccaccttg
     2341 tcccaggctt cgcggcgcca gtagacgccc ttgaggttgg cgagcatccg ggccaggaac
     2401 tgccggttgc ccaccggcac gaagtgctcc ggcgcgagcc gcaccggtcg gccgagggcc
     2461 cggctgacca gctcgcggca gctctcggtc gtcaggaggg cgccgcggtt gaagggatcg
     2521 agcagcacct gctctcccgc gatgcgggcg ccggtgatga agtggccggg cagcccgatg
     2581 ccctccatct cgagatcgag acggcggccc acctcgatga gcacgaggga cagcgtgatc
     2641 gggatgccga ggcggcgatt caggacctcg ttcaggtggc tgttgcgcgg gtcgaaatag
     2701 tcgtcgcggt cccccgagaa gcccagctct tcgaagaggt actcccggag ccgatgcagg
     2761 cggccgaggg cgtcgtcggc ccgccgggcc ggctcggcgg cctccgccag cgtggcgagc
     2821 cgcccgaggt agtcgtccat gtcgagatcg ggctgctcgc cggcggcgat caggagcgcg
     2881 gcgcgcgcca ggtggatctg gtcgtccggg cgatccaccg ccacggcgaa cgcggcgagc
     2941 gacgggtcgg cgtccatcaa cagccggggg cgtggtggat gccggcggtc cggagcgccg
     3001 cgccctgcag ctcttccgcg agcgccagcg tgcgcgcgga ggcccgccgg gccgcctcct
     3061 gcgtctccgg cgtggtggcc agcttgagca ggagcgagcg gccgagctgg tgatggaagc
     3121 gctcgtccgg ctcgatgatc tcgcgataga gcgtggcggt gagtcggtcg cccgcgcggt
     3181 cgcagaactc gatgaactga cggttcttca ccaccgcgat cgcctcgcgc gtgaactggc
     3241 cggccgcgac ccgctcgacg gtggtctcga gggaatcgag gtacttgaag agcggtccga
     3301 acccctgggc gagcggatcg aaggcgcgcg cgtcgaagcc catctccccg agccgctcgg
     3361 cgatcatccg gtagtgcttg gcctcgtcgc ccgcctggcg cgcgaaggcc atcttcacct
     3421 cgacgtcggt cgtggccacc agccagcgcg ccgcgatctc ggtggcctcg atctcgttct
     3481 tgagcgccac tttcaagaga ttcatgacgt tcagattgcc cgagacctcg ggcttgagcg
     3541 tggcctccgg ctcgaggcgg cgcaggacgg cctggttggc ctcgtcgagc tgtgcgacga
     3601 actcggcggc ggtcacgcgg cgaggccccg ctgcaggagc tcgggcagct cctgggtcag
     3661 cgtctccttg gtcaccaccc gccggcagcg ggcgtgcagc ggctgggtcg tccgcgcgag
     3721 ggcgtgggtg gtgtagccga gcaccggtgc ggcgggtacg cgctccaggg ccgtgaacat
     3781 cgcttcgtag tcccaaccgc tggtggtgag atccacgatg accaggacgg gcggctcgcc
     3841 ttccagggcc gcggccagct cctcgggggt gcgggcgaag accagcggag tgccgaccag
     3901 acgggcggtt tcccggatgc gggcgacgaa gaagagatcc cggacgacgg cgacggcctt
     3961 gatggggcgg ctccgcctac tcgtcctgct cctggacgcc gagccagtac ctgttccact
     4021 cggccaggtg cgtgagcgtc gacaggtcgc gcatgcggtc gacgtagacg atgccgttca
     4081 ggtgatcggt ctcgtgctgg atcacccgcg cgaagaactc cttggccacc acgtcgatcg
     4141 ggttgccctc gcggtcgcgc gcctcgagcc gcgccgaccc gaagcgcggg acgatgcccc
     4201 gcatgtcggg gacggagagg cagccctccc agccgtcttc cgtctcgtcg gtgagcgggg
     4261 tcacgaccgg gttgatcagg acggtcagcg ggatggcggg cgcgtcggga tagcgcgggt
     4321 tgccgtgcac ctcgatcacg cagatctgct tgagcgcgtg gacctgcggc gcggcgagcc
     4381 cggccccgtt gtattccctc atcgtctcga ccatgtcgtc gatcagccgc tgggtttccg
     4441 cggagaggat ctcgctcacc gggacggggg cggcgacctt gcgaagcacc gggtgtccga
     4501 gacgcgcgac tttcaggatg gccatgccga tctcctctcg cgttcggatt gtagcataga
     4561 ccctcacgcg ccctggaacc cgtcgaggac ggcgggccag cgtggatcgt cgagcgaggg
     4621 ctggaaggac gggtagagct gcgtgcggtc ttagaggccg cgatagcgct cgcggatgcg
     4681 ctccccgatg gtggcgaagg acccgctcac cgcgaagtga tccagggtct cgtcgctgat
     4741 gagatcggcc atgccctccc agtcgcccgc gagcgacttc gcgtgcaggc gcgggacgag
     4801 atcctcgcgc ccgtgaatcg cgagcaccgc ctggtaggtt cgcgtggatg cgtagaaaac
     4861 gatctgcttc ttcaccgccc gcgctcttcc ggatttcctc ctcggtgtcg cccagcacca
     4921 ccatggtcga ggcgcggaag gtgaagtcgg cccgcgagcg accccctcgg ttcagtccct
     4981 cctgcaccgc ggggtgcacg cactcgcgca gctagcgcgc gctgtggaag gagtggatgt
     5041 gcagcccgtc cgcgacctcg cccgcgatcc ggcacatctg cgggttcacc ccggccaggt
     5101 agatcgggat gagcggatgc tcgatgggcc ccgggttgaa gaagggcgtc atgagatcga
     5161 agtggtagga gcgcctgcgg aaatcgagcg gggtgccccg ctgccagcag tcccagatcg
     5221 cccgcagcgc ctggacgacc tcgcgcatcc g
//
LOCUS       DASUFA010000307         6773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1655061, whole genome shotgun sequence.
ACCESSION   DASUFA010000307 DASUFA010000000
VERSION     DASUFA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655061"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /gene="mutS"
                     /locus_tag="VFX87_11505"
     CDS             <1..1929
                     /gene="mutS"
                     /locus_tag="VFX87_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX4995593.1"
                     /translation="LGVGTLTAGLQAAGAALAYLRETQGESLAHLTRIQRLVTGEAML
                     LDPTAVATLELFETAQERTARGSLFAALDATSTPMGARLLRQWMLRPLLDREAIGRRQ
                     EAVAALVDAPAARDALQRRLEGVGDLERLTSRAALGVAHARDLIALRAFLGRLPGLAE
                     ALGALDGALLGTLGEEITPLPDLQKLLEEALEDEPPLALKEGGLIREGWNAELRELKQ
                     AARQGKEWIASLEQRERARTGIASLKVRFNRVFGYAIEVSNAHGAKVPSDYLRRQTLV
                     GAERYVTAELKEYESRVLGAEERIGRLEFELFGQVRAEVAAAAGPLLRTARAVGSLDA
                     LASLAGVAHQRGYVRPQVDDGFALEIVDGRHPVLEAAGTPFTPNDVTLDPAGCQIMIL
                     TGPNMSGKSVFMRQVALLALMAQMGSFVPARSARIGLVDRILTRVGAQDNMARGQSTF
                     LVEMVETASILHHATERSLILLDEVGRGTSTFDGLAIAWAVTEELHERGRGAKVLFAT
                     HYHELTALAERLPRVHNFHVAVREWNDEIIFLHKVRPGGTDRSYGIQVARLAGLPPLV
                     IARAKAILGELERERGELQSAVTPEAPATGPAQLGLFPPTHDPLLRELAALDLGAMTP
                     LQALNLLADWQQRLKARP"
     gene            1926..3686
                     /gene="mutL"
                     /locus_tag="VFX87_11510"
     CDS             1926..3686
                     /gene="mutL"
                     /locus_tag="VFX87_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX4995594.1"
                     /translation="MSGPRIRRLPDHLVNKIAAGEVVERPGSVVKELVENALDALCTQ
                     VTVDLRDAGRALIRVTDDGIGMSSEEVDLALLRHATSKLATDADLDAITTLGFRGEAL
                     PAICAVTRFSVLSCPRGAETGTLVRGEGGTVNEKLLVAAPAGTTVEAQDLFFNTPARL
                     KFMKSAQSEVAAALRLLEAIALAHQDVHFRISHNGRPALAAPRARSLRDRIGALWGFE
                     RAGKLLEVERKDGALAVSGLIAPPQLARGNRDEIVLIVNGRPVRDTQLSQTLIEAYRP
                     LLARDQFPVAVLHLELPPQEVDVNVHPTKAWVRFRSPRQIQEILYRAVQDALRQAQVV
                     QSQRGLGPVGSGKAAGDAAGSFSIESGGPGMAPALPRSADAPTSAGQALLFHEAPAHF
                     PAVGFGAVIGQLQDTFIVSASDDEIFFLDQHVAHERVLFERLLGDLDAGPLASQELLF
                     PQPVELGAGQAALVQEWAETLEGLGFRLEGWSGATVVLRAVPALLRGQEPRRLIEALV
                     EDVNRPRKGEAVPLLHRALSFVACHAAIKAHAPLQREEMARLIADLAITKTPYFCPHG
                     RPIVSRLSLRDIKRELGRTW"
     gene            3683..4630
                     /gene="miaA"
                     /locus_tag="VFX87_11515"
     CDS             3683..4630
                     /gene="miaA"
                     /locus_tag="VFX87_11515"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX4995595.1"
                     /translation="MSRPPLVVLVGPTGIGKTAVAVRLARQIAMEVVGADSRQVYRGM
                     DIGTGKPTVEECAAVPHHLIDVVDPDERYHAARFRSDALAAIAAVQGRGRLPVVVGGT
                     GLYVRALLRGLAPAPPADPTLRAELEGFAAEHGAPALHARLAAVAPDAARRLHPNDRV
                     RVVRALEVHAAAGAATRDTGAGDWAGTDGAWRLVMIGLGRARPVLHAALAERARGMLA
                     RGMMDEVRRLLEAGHDETRPPMDGIGYRQLALALRGQMTVADALRLMIRDTTRYAKRQ
                     MTWFQREPEIRWLDVDEAGGVEGAAEAVHKHITREGLIE"
     gene            4627..5877
                     /gene="hflX"
                     /locus_tag="VFX87_11520"
     CDS             4627..5877
                     /gene="hflX"
                     /locus_tag="VFX87_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX4995596.1"
                     /translation="MRSREKAVLAALRLPSQPRVVVEESLDELGRLAESAGALVVGRA
                     TQDRRAPTPGLYFGKGKVEEIKTLSKREGADLMISDDSLSPIQERNLGGSLGVRVIDR
                     TALILDIFAQRARTMEGKLQVELAQLSYLLPRLVGQWKHLERTGGGIGTRGPGEMQIE
                     SDRRMIRHRIQKIRDELQRVRTHRRLLRDRRKAAGVPVVALVGYTNAGKTTLLNRLTG
                     ADRVAADQLFVTLDPTARLVSSPAHASFILTDTVGFIRKLPTQLVAAFKATLEELTEA
                     DVLVHVVDASHPGLDDQVQAVEALLGELELMGRPMVVALNKIDRLEPGAARALLARFD
                     GVAISARTGEGVDALLTRLDEALRPRVARLILRIPYADGPALAACYERGRVLSRRDEP
                     EGIRLEVELPRRVLPSLEGYRVTG"
     gene            5961..6515
                     /gene="pgsA"
                     /locus_tag="VFX87_11525"
     CDS             5961..6515
                     /gene="pgsA"
                     /locus_tag="VFX87_11525"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX4995597.1"
                     /translation="MGLPNWLTILRILLIPVFVTLLVYRRAGLALLVFCLASLTDMLD
                     GYIARSQGRQTRLGAFLDPMADKLLLTSGFVTLTWLKVIPFWITAVVVSRDVVLSIGV
                     LVIHVAGGTVHPAPTWIGKMSTLFQMLTVLVAMAAVYFQLLPAFVTTLLAWVTAAFTI
                     TSGLQYIIRGLKQLETHNSVSGRV"
     gene            6517..>6773
                     /locus_tag="VFX87_11530"
     CDS             6517..>6773
                     /locus_tag="VFX87_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4995598.1"
                     /translation="MARRPDPAAEGVVVAAVTPRTPAARAGLRRGDRILAINGAALRD
                     VIDFHVHAGDEHLRLSIAREGQARTARLRRGAAGLGLELEAP"
BASE COUNT          908 a         2461 c         2478 g          926 t
ORIGIN      
        1 ctgggcgtgg gcaccctcac cgcgggcctg caggccgccg gggccgcgct cgcctacctg
       61 cgcgagaccc agggcgagtc gctggcgcac ctcacgcgga tccagcgcct ggtgaccggc
      121 gaggcgatgc tgctcgatcc gaccgcggtc gccacgctcg agctctttga aaccgcccag
      181 gagcgcacgg cccggggctc gctcttcgcc gcgctcgacg ccaccagcac gcccatgggc
      241 gcccggctgc tccgccagtg gatgctccgc ccgctgctcg accgcgaggc catcggccgc
      301 cgccaggagg cggtggccgc cctggtggac gccccggctg cccgggatgc cctgcaaagg
      361 cgcctggagg gcgtggggga cctggaacgg ctcacgagcc gcgcggccct gggcgtggcc
      421 catgcccgcg atctcatcgc cctgcgggcc ttcctgggcc gcctgccggg gctcgcggag
      481 gccctgggcg cgctggacgg cgcgctcctg ggaaccctgg gcgaggagat cactccgctg
      541 cctgatcttc agaaactact tgaagaggcg ctggaggacg agccgccgct cgccctgaag
      601 gaaggcggcc tgatccggga gggctggaac gcggagctgc gggagctgaa gcaggccgcc
      661 cggcagggca aggagtggat cgcctcgctc gagcagcggg agcgcgcgag aaccggcatc
      721 gcctccctga aagtccgctt caatcgagtc ttcggctacg cgatcgaggt gagcaacgcg
      781 cacggcgcca aggtgccctc cgactacctg cgccggcaga ctctggtcgg ggcggagcgc
      841 tacgtcaccg ccgagctgaa ggagtacgag agccgcgtgc tcggcgccga ggaacgcatc
      901 gggcgcctcg agttcgagct gttcggccag gtgcgcgcgg aggtcgcggc ggccgccggc
      961 ccgctgctcc gtacggcccg tgcggtcggc agcctcgacg ccctcgcctc gctcgccgga
     1021 gtggcccatc agcgcggcta cgtgcggccc caggtggacg acggcttcgc gctcgagatc
     1081 gtggacggcc gccatccggt cctggaagcg gccggcacgc cgttcacccc caacgacgtg
     1141 accctcgatc cggcgggctg ccagatcatg atcctgaccg gcccgaacat gagcggcaag
     1201 agcgtgttca tgcggcaggt cgccctgctc gccctcatgg cccagatggg aagcttcgtc
     1261 ccggcccgct cggcccggat cggcctggtg gaccgcatcc tcacccgcgt gggcgcgcag
     1321 gacaacatgg cacgcgggca gagcacgttc ctggtggaga tggtggagac ggccagcatc
     1381 ctgcaccacg ccaccgagcg gagcctgatt ctcctggacg aggtcgggcg cggcacgtcc
     1441 accttcgacg gcctcgcgat cgcgtgggcg gtgaccgagg agctgcacga gcgcggccgc
     1501 ggcgccaagg tgctcttcgc cacgcactac cacgagctga ccgcgctggc cgagcggctg
     1561 ccgcgggtgc acaatttcca cgtggcggtc cgcgagtgga acgacgagat catcttcctg
     1621 cacaaggtgc ggccgggcgg caccgaccgt tcctacggca tccaggtggc gcggctggcc
     1681 gggctgccgc cgctggtgat cgcgcgggcc aaggccatcc tgggcgagct cgagcgcgag
     1741 cgcggggagt tgcagtcggc ggtaacgccc gaggcgcccg cgaccggccc cgcccagctc
     1801 gggctcttcc cgcccaccca cgatccgctc ctgcgggagc tggccgcgct ggacctgggc
     1861 gcgatgaccc cgctgcaggc gctgaacctg ctcgcggact ggcagcagcg cctcaaggcg
     1921 cggccgtgag cggcccccgc atccggcggc tgcccgacca cctcgtcaac aagatcgcgg
     1981 ccggggaggt ggtggagcgg cccggctcgg tggtgaagga gctggtggag aacgcgctcg
     2041 acgcgctgtg cacccaggtg acggtggacc tgcgcgacgc gggccgcgcc ctgatccgcg
     2101 tcaccgacga cggcatcggg atgtcgagcg aggaggtgga cctggccctg ctgcgccacg
     2161 ccacgtccaa gctcgccacc gacgccgacc tcgacgccat caccacgctg ggcttccggg
     2221 gcgaggcgct gcccgcgatc tgcgcggtca cccgcttctc ggtgctctcg tgcccgcgcg
     2281 gcgcggagac cggcaccctc gtgcgcggcg agggcggcac cgtcaacgag aagctgctgg
     2341 tggcggcgcc cgcgggcacc acggtggagg cgcaggatct cttcttcaac acgcccgcgc
     2401 ggctcaagtt catgaagagc gcgcagagcg aggtggcggc ggcgctgcgg ctgctcgagg
     2461 ccatcgcgct ggcgcaccag gacgtgcact tccgcatctc gcacaacggc cggcccgccc
     2521 tcgcggcgcc gcgggcccga agcctgcgcg accgcatcgg ggcgctgtgg ggcttcgagc
     2581 gcgcgggcaa gctgctggag gtggagcgca aggacggcgc cctcgcggtc tcgggcctga
     2641 tcgcgccgcc ccagctcgcc cgcggcaacc gcgacgagat cgtgctcatc gtcaacggcc
     2701 gccccgtgcg cgacacccag ctcagccaga ccctgatcga ggcctaccgg ccgctcctgg
     2761 cgcgcgatca gttcccggtc gccgtgctcc acctcgagct gccgccccag gaggtcgacg
     2821 tcaacgtcca cccgaccaag gcctgggtcc gcttccgttc cccgcgccag atccaggaga
     2881 tcctctaccg cgcggtgcag gacgcgctgc gccaggcgca ggtcgtccag tcccagcggg
     2941 ggttggggcc ggtgggctca ggcaaggcgg caggcgatgc cgccggaagc ttctctattg
     3001 aaagcggggg cccaggaatg gcccccgcac tcccccggtc cgcggacgct ccgacttctg
     3061 ccgggcaggc gttgctcttt cacgaggccc cggcacactt tcccgcggtc ggcttcggcg
     3121 ccgtgatcgg gcagctacag gacaccttca tcgtctccgc gagcgacgac gagatcttct
     3181 tcctggacca gcacgtggcc cacgagcgcg tgctgttcga gcggctgcta ggcgatctgg
     3241 acgcgggccc cctggcttcg caggaattgc tcttcccgca gcccgtggag ctgggcgcgg
     3301 gccaggcggc gctggttcag gagtgggccg agaccctcga gggcctgggc ttccggctcg
     3361 agggctggag cggcgccacg gtcgtgctcc gcgcggtacc cgcgctgctc cgagggcagg
     3421 agccgcggcg gctgatcgaa gcgctggtgg aggacgtcaa ccgcccgcgc aaaggcgagg
     3481 cggtgccgct gctccaccgc gcgctgtcct ttgtggcctg ccatgcggcg atcaaggccc
     3541 acgccccgct gcagcgcgag gagatggcgc ggctcatcgc ggatctggcc atcacgaaaa
     3601 cgccgtactt ctgccctcac ggccggccga tcgtcagtcg cctgtcttta cgcgacatca
     3661 agcgggagct ggggcgcacg tggtgagccg gccgccgctg gtcgtcctgg tggggccgac
     3721 gggcatcggc aagaccgcgg tcgcggtgcg gctcgcccgg cagatcgcca tggaggtcgt
     3781 cggggcggac tcgcgccagg tctaccgcgg catggatatc ggcaccggca agcccaccgt
     3841 ggaggagtgc gcggcggtgc cgcaccacct gatcgacgtc gtggatcccg acgagcggta
     3901 ccacgccgcc cgcttccgct ccgacgcgct cgccgcgatc gcggcggtgc agggccgcgg
     3961 gcgcctgccc gtcgtggtcg ggggcaccgg cctctacgtg cgcgcgctgc tccgcggcct
     4021 ggcgcccgcg ccgcccgccg atcccacgct gcgggccgag ctcgagggct tcgcggccga
     4081 gcacggggcg ccggcgctgc acgcgcggct cgccgcggtg gcgcccgacg ccgcgcgccg
     4141 cctgcatccc aacgatcggg tccgcgtggt ccgggcgctg gaggtccacg ccgcggccgg
     4201 cgcggccacg cgagacaccg gagccggcga ctgggccggc accgacgggg cctggcgcct
     4261 cgtcatgatc ggactcggcc gcgcgcgccc ggtgctccac gcggccctcg cggagcgcgc
     4321 gcgcgggatg ctcgcccgtg gcatgatgga cgaggtgcgg cgcctcctcg aggccggcca
     4381 tgacgagacg cggccgccga tggacggcat cggctaccgg cagctcgcgc tggcgctgcg
     4441 agggcagatg acggtggcgg acgcgctgcg gctcatgatc cgcgacacca cccgctacgc
     4501 gaagcggcag atgacgtggt tccagcgcga gccggagatc cgctggctcg acgtggacga
     4561 ggcgggcggc gtggagggcg cggccgaggc ggtgcacaag cacatcacac gggaggggct
     4621 gatcgagtga ggtcacgcga gaaggcggtg ctggcggccc tgcggctgcc gagccagccg
     4681 cgggtggtgg tggaggagag tctcgacgag ctcgggcgcc tggcggagtc ggccggcgct
     4741 ctcgtggtgg gccgggcaac ccaggaccgg cgcgcgccga ccccgggcct ctacttcggc
     4801 aaggggaagg ttgaggagat caagaccctc tcgaagcgcg agggcgccga cctgatgatc
     4861 tccgacgatt cgctctcgcc catccaggag cgcaatctcg gcggctcgct cggcgtgcgg
     4921 gtgatcgacc gcaccgcgct catcctcgac atcttcgccc agcgcgcccg caccatggag
     4981 ggcaagctcc aggtggagct ggcccagctc tcctacctac tgccgcggct ggtggggcag
     5041 tggaagcatc tggagcgcac cggcggcggt atcggcacgc ggggaccggg cgagatgcag
     5101 atcgagtccg accggcgcat gatccggcac cgcatccaga agatccgcga cgagctgcag
     5161 cgggtgcgca cgcaccggcg gctgctgcgc gaccggcgga aggcggcggg cgtgccggtg
     5221 gtcgccctgg tgggctacac caacgcgggc aagaccactc tgctcaatcg cctgaccggc
     5281 gccgaccgcg tcgcggccga ccagctcttc gtgacgctgg atccgacggc ccgcctggtg
     5341 tcgagcccgg cccacgcctc gttcatcctg accgacacgg tcggcttcat ccgcaagctg
     5401 cccacccagc tcgtggccgc gttcaaggcc acgctggagg agctgaccga ggccgacgtg
     5461 ctggtgcacg tggtggacgc gagccatccc gggctcgacg accaggtcca ggcggtggag
     5521 gcgctgctgg gcgagctcga gctcatgggg cgccccatgg tcgtcgccct caacaagatc
     5581 gaccggctcg agcccggcgc ggcccgcgcg ctgctcgccc gcttcgacgg cgtcgcgatc
     5641 tcggcccgca cgggcgaggg cgtcgacgcg ctgctgacgc ggctcgacga ggcgctccgg
     5701 ccgcgagtgg cgcggctcat cctccgcatc ccgtacgcgg atgggcccgc gctggccgcc
     5761 tgctacgagc gcggccgcgt gctctcgcgt cgcgacgagc cggaaggaat tcgtctggaa
     5821 gtcgagctgc cgcggcgggt gctgccctcg ctcgaggggt accgggttac cgggtaaccg
     5881 ggacccgccc tctcccctga ggggagaggg ggagcctccg gcggtgctac aatcggacct
     5941 cggccctcgc ggaccgttcc atgggacttc ccaactggct cacgatactg cgcatcctgc
     6001 tgatcccggt cttcgtgacc ctgctcgtct atcggcgggc ggggttggcg ctgctcgtgt
     6061 tctgcctggc cagcctcacc gacatgctgg acggctacat cgcccgcagc cagggtcgcc
     6121 agacgcggct cggagccttc ctcgatccga tggccgacaa gctgctgctc acctccggct
     6181 tcgtgacgct gacctggctc aaggtgatcc cgttctggat caccgcggtc gtcgtgagcc
     6241 gtgacgtggt gctgagcatc ggcgtgctgg tcatccacgt ggcgggcggc acggtgcatc
     6301 cggcgccgac ctggatcggc aagatgtcca ccctgttcca gatgctcacc gtgctcgtgg
     6361 ccatggccgc cgtctacttc cagctcctgc ccgcgttcgt caccacgctc ctggcctggg
     6421 tgacggccgc gttcacgatc acctcggggc tgcagtacat catccgcggg ctcaagcagc
     6481 tcgagacgca caactccgtg agcgggcgcg tctgacatgg cccggcgacc cgacccggcg
     6541 gcggagggcg tggtggtggc cgcggtgacg ccgcggacgc cggcggcccg ggccggcctg
     6601 cgtcgcggcg accgcatcct cgccatcaac ggcgccgcgc tgcgcgacgt catcgacttc
     6661 cacgtccacg cgggcgacga gcacctgcgc ctctcgatcg cgcgggaggg gcaggcccgg
     6721 accgcgcggc tgcgccgggg cgccgcgggg ctcgggctcg agctcgaggc ccc
//
LOCUS       DASUFA010000308         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1319928, whole genome shotgun sequence.
ACCESSION   DASUFA010000308 DASUFA010000000
VERSION     DASUFA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319928"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFX87_11535"
     CDS             complement(<1..179)
                     /locus_tag="VFX87_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX4995599.1"
                     /translation="MKPAPFEYHRPVSLAETFDLLDRYGDDGRLLAGGQSLVPALNLR
                     LAAPRAVIDINRIPDL"
     gene            complement(176..1165)
                     /locus_tag="VFX87_11540"
     CDS             complement(176..1165)
                     /locus_tag="VFX87_11540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4995600.1"
                     /translation="MPTDIALTSLAMPLSEFPAIAREAEQRGYRTAWVGEASGAEAIV
                     LSTLIATHTQTLKIANGVIPVQTRTPIVYGQAAATLGHLAPGRFALGLGLSSEIIVGQ
                     WHGLPFTPSIQQMREAVQIIRMAAAGERVSFEGKFYRLKNFRLAIPAPSPAPRIYLAA
                     LGPRMCELAGEVADGVLLNWIPPSAVPASVRHVETGARRAGRSLSDVDVAVYVRTCVT
                     DEPEPARETLARDITGYAIVSVYARFFEECGFAEEVLAVNRAWKAGDRAGAVKEISER
                     VLQGLGAVGSAEACRAQIAAFARTGATPVVLPFAPPGPAARASLLRTFRSFPA"
     gene            complement(1165..1788)
                     /locus_tag="VFX87_11545"
     CDS             complement(1165..1788)
                     /locus_tag="VFX87_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995601.1"
                     /translation="MSPHALYLVRMDVAHDLEPVFNDVYDKEHLPLLQAVPGVVRGSR
                     YRQPSPTEPRYLAAYELESAGVLQSPQWKTAGEAGRWPTEIRPNTMNRHLATYEWVGS
                     SPALTYRTPYIFWVMMDIEPHREALFNELYDVEHVPLLLKLSGCVNVVRYRTETPGEP
                     RYLAAYEVDRPDLPMSKLWNDTSDIGRWKPEVRPYTYNKRYIVSERI"
     gene            complement(1822..2973)
                     /locus_tag="VFX87_11550"
     CDS             complement(1822..2973)
                     /locus_tag="VFX87_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX4995602.1"
                     /translation="MNLTPFVVDVAQASLDDLRERLARTRWPDEVDGAGWDYGASLAY
                     MRELVEYWRTGFDWRGVERAMNTVPHFRAQVDGIGIHVIRQRGRGPAPMPLLITHGWP
                     SSFVEMLALIPILADPGAHGGDPADAFDVVVPSVPGFGFSDRPGRGMTRSRVAGLWAG
                     LMEGLGYSRYAAHANDIGAVISAWLAADHPDRLIALHTLMPTFPPAVIGADARPLTPA
                     EQDFARLQERWDREEGGYNRIQETRPQTLAYGLHDSPAGLAAWIVEKWLAWTGEAGAV
                     ARRFDRDLLLANVTLYWVTGTANAANRSYYERARETRRITSRITVPTGVALTSEAIQR
                     PPRELAERSFADIRRWVDLPRGGHFVAGEAPEVLARELREFFRPFRRAG"
     gene            3182..>3644
                     /locus_tag="VFX87_11555"
     CDS             3182..>3644
                     /locus_tag="VFX87_11555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4995603.1"
                     /translation="MDGQTQIFHEEAFLHLLNREAVRATRYQDFFSVCLLRPDGPGDR
                     WRRSEEVERALSQKIPEFVRATDMVGQLSSAIAVILLHTAGEDALRVAERIRTSIEQV
                     AFRESSDGPPQRLTVSVGKVSFPQDGHDDAALLSQALAFLDQATHRGGNRVT"
BASE COUNT          526 a         1295 c         1309 g          514 t
ORIGIN      
        1 agatccggga tgcggttgat gtctatgacc gcgcgcggcg cggccaggcg caggttgagc
       61 gcgggcacca ggctctggcc gccggccagg aggcgcccgt cgtcgccgta gcgatcgagg
      121 agatcgaagg tctcggcgag agacaccggc cggtgatact cgaacggggc gggcttcatg
      181 ccgggaacga gcggaaggtc cgcaggagcg acgcgcgcgc ggcggggccg ggcggcgcga
      241 acggcagcac gaccggggtc gccccggtgc gcgcgaacgc cgcgatctgg gcgcggcagg
      301 cctcggccga gcccaccgcg cccaaaccct ggagcacgcg ctccgagatc tccttcaccg
      361 cgcccgcgcg atcgcccgcc ttccaggcgc ggttgacggc taggacctcc tcggcgaagc
      421 cgcactcctc gaagaagcgc gcgtagacgc tcacgatggc gtagccggtg atgtcgcggg
      481 ccagcgtctc gcgcgccggc tcgggctcgt ccgtcacgca ggtgcgcacg tacaccgcga
      541 cgtccacgtc cgacaggctc cggccggcgc gccgggcccc ggtctccacg tggcgaaccg
      601 aggcgggcac cgcggacggc gggatccagt tcagcagcac cccgtccgcc acctcaccgg
      661 ccagctcgca catccggggg ccgagcgcgg cgaggtagat ccgcggcgcg ggcgacgggg
      721 cgggaatcgc gagccggaaa ttcttgagcc ggtagaactt gccctcgaag ctcacccgct
      781 cgcccgccgc ggccatccgg atgatctgca ccgcctcccg catctgctgg atggacggcg
      841 tgaacggcag cccgtgccac tgccccacga tgatctcgct ggagagcccg aggccgaggg
      901 cgaaccggcc cggggccagg tgcccgagcg tcgcggcagc ctggccgtac acgatcggcg
      961 tgcgcgtctg caccgggatc accccgttgg cgatcttgag cgtctgcgtg tgcgtcgcga
     1021 tcagggtgga cagcacgatg gcttccgcgc ccgaggcctc acccacccag gcggtgcggt
     1081 acccccgctg ctccgcctca cgggcaatcg cagggaactc gctgagcggc atggcgaggg
     1141 aggtcagggc gatgtcggtg ggcatcagat gcgctcgctc acaatgtacc gcttgttgta
     1201 ggtgtatggt ctgacctcgg gcttccagcg gccgatgtcg gacgtgtcgt tccacagttt
     1261 cgacatcggc agatcgggcc ggtccacctc gtaggcggcc aggtagcgcg gctcgcccgg
     1321 cgtctcggtc cggtagcgca ccacgttcac gcagccggac agcttgagga gcagcggcac
     1381 gtgctcgacg tcgtagagct cgttgaacag cgcctcgcgg tggggctcga tgtccatcat
     1441 gacccagaag atatagggcg tccggtaggt gagcgccggg ctcgacccca cccattcata
     1501 ggtcgcgaga tggcggttca tggtgttggg ccggatctcc gtgggccagc ggcccgcctc
     1561 ccccgccgtc ttccactgcg ggctctggag cacccccgcg ctctcgagct cgtaggccgc
     1621 gaggtagcgc ggctcggtcg gcgagggctg acgatagcgc gagccccgga cgaccccggg
     1681 caccgcctgg agcagcggca ggtgctcctt gtcgtacacg tcgttgaaga cgggctcgag
     1741 gtcgtgtgcc acgtccatcc ggaccaggta gagggcatgt gggctcatcg tggtgcctcc
     1801 cggcggagca ctatctcaca gtcaaccggc cctgcggaac ggccgaaaga actcgcggag
     1861 ctctcgggcg agcacctccg gcgcttcgcc cgccacgaag tggccgccgc ggggcagatc
     1921 cacccagcgg cggatgtcgg caaagctgcg ctcggcgagc tcccgcggcg ggcgctggat
     1981 ggcctcgctc gtgagggcga ccccggtggg caccgtgatg cgcgaggtga tgcgccgggt
     2041 ctcccgggcc cgctcgtagt acgagcgatt cgccgcgttg gcggtcccgg tgacccagta
     2101 gagcgtgacg ttggccagga gcagatcgcg gtcgaagcgg cgcgcgaccg ctccggcctc
     2161 gcccgtccac gcgagccact tctccacgat ccaggccgcg agccccgccg gcgagtcgtg
     2221 gagcccgtag gccagcgtct ggggccgggt ctcctggatc cggttgtagc cgccctcctc
     2281 ccggtcccag cgctcctgca ggcgcgcaaa gtcctgctcg gccggggtga gcggccgcgc
     2341 gtcggcgccg atcacggcgg gcgggaaggt cggcatcagc gtgtgcagcg cgatcaggcg
     2401 gtcgggatga tcggccgcga gccaggcgct gatcaccgcg ccgatatcgt tggcgtgcgc
     2461 ggcgtagcgc gaatagccga gcccctccat gagacccgcc cagagcccgg ccacgcggct
     2521 ccgggtcatc ccgcggccgg gccggtcgga gaagccgaag ccgggcaccg acggcacgac
     2581 cacgtcgaag gcatcggcgg ggtcgccgcc gtgcgccccc gggtcggcca ggatcgggat
     2641 cagcgcgagc atctcgacga aggagctggg ccagccatgg gtgatgagca gcggcatcgg
     2701 cgccggcccc cgcccgcgct ggcggatcac gtggatgccg atgccgtcca cctgggcgcg
     2761 gaagtgcggc accgtgttca tcgcccgctc cacgccgcgc cagtcgaacc cggtgcgcca
     2821 gtactcaacc agctcgcgca tgtaggcgag gctcgcgccg tagtcccagc ccgcgccgtc
     2881 cacctcgtcc ggccagcgcg ttctcgcgag ccgctctcgc agatcgtcga ggctcgcctg
     2941 cgccacgtcc accacgaacg gtgtgagatt cattcgccgc ctccgggccc agcctgccac
     3001 aggtcacccg ggatatgacg gccatcaggc ccccgttccg gggcgagccc cgtgcggggt
     3061 ccacgcgatc ggcgccggct ccgcccggcg ccgggattgc ccccggcccc gttccgtagt
     3121 tcgaccggat ctcggaccgg gaggagtaac atggccccgg gggcgactcg tgggcgaact
     3181 catggatggc cagactcaga ttttccatga agaagcgttc ctccatctcc tgaaccggga
     3241 ggccgtgcgc gccacccggt atcaggactt cttctcggtc tgcctgctgc ggccggacgg
     3301 ccccggcgac aggtggcgcc gctcggagga agtcgagcgg gccctgtccc agaagatccc
     3361 ggagttcgtc cgggccaccg acatggtggg gcagctctcc tcggccatcg cggtcatcct
     3421 gctgcatacc gccggggagg atgccctgcg ggtcgccgag cggatccgca ccagcatcga
     3481 gcaggtggcg ttccgggaga gctcggacgg gccgcctcag cgcctcaccg tgagcgtggg
     3541 gaaggtctcg ttcccgcagg acggtcacga cgatgccgcg ctcctgtccc aggcgctggc
     3601 cttcctggac caggccacgc atcggggcgg caatcgggtg accg
//
LOCUS       DASUFA010000309         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_402628, whole genome shotgun sequence.
ACCESSION   DASUFA010000309 DASUFA010000000
VERSION     DASUFA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402628"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFX87_11560"
     CDS             complement(<1..223)
                     /locus_tag="VFX87_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4995604.1"
                     /translation="MIDAALLPALLSLLTVARSGSVAAAARLHHRTSSAISQQIRKLE
                     RHLGVTLLERAGRGVRLTAAGEAALPGILK"
     gene            323..1726
                     /gene="leuC"
                     /locus_tag="VFX87_11565"
     CDS             323..1726
                     /gene="leuC"
                     /locus_tag="VFX87_11565"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX4995605.1"
                     /translation="MASTLLDKVWDAHTVRTLPSGQTQLLIGLHLIHEVTSPQAFQAL
                     QELKLPVRMPGRTFATLDHIIPTTAQQRPFADEVAEEMAVHLTRNSEKSGVSFFGLDS
                     GRQGIVHVIGPELGLTQPGMTIACGDSHTATHGAVGAVAFGIGTSQVRDVLATQCLAM
                     AKPKLRQVRVDGALSKGVYAKDVILAIIRTLGVNGGVGYAYEYAGAVVERMTMEERLT
                     LCNMSIEGGARSGYVNPDQITFDYLRGRQYAPQGAAFDRAVTWWKGMASDANAAYDDV
                     RRLDGNAIPPMVTWGINPGQAIGVGERIPSPESAPEPERATYREGLAHMGFEAGQPIA
                     GAKIDVVFIGSCTNSRISDLRVAAAVAKGNRVAKGVRAIVVPGSQMVAKVAEAEGLHE
                     VFREAGFEWRKAGCSMCLGMNDDKLRGREMSASTSNRNFIGRQGSPTGRTLLMSPAMA
                     AAAALNGAVTDVREMMR"
     gene            1723..2316
                     /gene="leuD"
                     /locus_tag="VFX87_11570"
     CDS             1723..2316
                     /gene="leuD"
                     /locus_tag="VFX87_11570"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX4995606.1"
                     /translation="MSDSARRQIAGRGIPIPGNDIDTDRIIPARFLKTVTNEGLGLHA
                     FEDARKHDPEHPFNQKAYEGASVLVAGMNFGCGSSREHAPESLKQWGIKAIVGGSFGE
                     IFFGNCTMLGIPCLSISQDDVLWLQRAVGRDPKQAVTVDVERQEVRVGDRVIPARIPD
                     GARNQLVSGAWNATGVLLDAGEAIEATAGRLPYVKGF"
     gene            2334..3227
                     /locus_tag="VFX87_11575"
     CDS             2334..3227
                     /locus_tag="VFX87_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010211540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4995607.1"
                     /translation="MRTVRTPTLEIGYEEWGEAGGAPVVLLHGFPDDAHAWDGVAPAL
                     AARGCRVLAPYLRGYGPTRFLDAGAPRLAQQAAIGQDLLDFLDALGIERAGLAGYDWG
                     GRAACITAILAPARVRALVTIGGYNVQNTIAPPVPASALQERANWYQWYFNTERGRLG
                     LEQNRREICRLLWRDWSPGWRFDDATFELAAAAFDNPDFVPVVIHSYRHRHRNAPGEP
                     RFDAIERRLAGRPRIEVPSVILHGRDDGVDPPARSEKHPALFPAGTERYVIPDAGHFL
                     PREQPGAVVDALLPLLARTAR"
     gene            3240..4097
                     /locus_tag="VFX87_11580"
     CDS             3240..4097
                     /locus_tag="VFX87_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4995608.1"
                     /translation="MLAVLAALCQVLRNTVMKRLGHSLDEYINVWGRFTFLLPFAGAF
                     VLWHGVPELKPGFYLACASFAVCQTISTMALSKALKLAEISMVTALWKVSLLILVVLG
                     YFTLREQPTLLGIAGILISMSGVYLLNVNRAHIAWWAPLAVLFTDRGMRYTLLAALFF
                     APSVVTIKWAMQCSDQYMGTLGGYVAASLLVTPIVLGTSRRRFVEVPRYWKAFVSFGL
                     FASLTTISQGTAYLMTLSSYVEAVKQVEILFAMAIGTVMFHEKQKVRDIAPGALVMLA
                     GVVLLSLAG"
     gene            complement(4094..4459)
                     /gene="queF"
                     /locus_tag="VFX87_11585"
     CDS             complement(4094..4459)
                     /gene="queF"
                     /locus_tag="VFX87_11585"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX4995609.1"
                     /translation="MPTQPSKALEVVPNPHPDRDYEISLEIPEFTCVCPRTGQPDFAT
                     IRVRYVPDRHLLELKAIKLYIWSYRNEGAFHEDVTNRILNDFVAAAAPRWIEVIGDFT
                     VRGGIKTVVRATHGKRPEI"
BASE COUNT          656 a         1571 c         1629 g          677 t
ORIGIN      
        1 gcttgaggat gccgggcagg gcggcctcgc ccgctgcggt gagccggacg ccgcggcccg
       61 cgcgctcgag gagcgtcacg ccgagatggc gctcgagctt gcggatctgc tggctgatcg
      121 cggaggacgt gcgatggtgc aaccgcgccg ccgccgccac cgagccgctg cgcgcgaccg
      181 tcagcaggct cagcagggcg gggaggagcg ccgcgtcgat cattagcgga tcttacggga
      241 ccagcaagaa tatttcacta tacttaacgg tctcgcggcg gcacactcac ccggccggcg
      301 tggtccggcc acggaggaga caatggcgtc aacgctactc gacaaggtgt gggacgctca
      361 cacggtgcgc acgctgccct cggggcagac gcagctcctg atcgggctcc atctcatcca
      421 cgaggtcacc tcgccccagg cgttccaggc tctgcaggag ctcaagctgc cggtgcggat
      481 gccggggcgg accttcgcga cgctcgatca catcatcccc accaccgcgc agcagcggcc
      541 gttcgcggac gaggtggcgg aggagatggc ggtacacctc acccggaact cggagaagag
      601 cggggtgtcg ttcttcggtc tcgacagcgg ccggcagggc atcgtgcacg tgatcgggcc
      661 cgagctcggg ctgacccagc cgggcatgac catcgcctgc ggcgacagcc acaccgcgac
      721 ccacggggcg gtgggagccg tcgcgttcgg catcgggacc agccaggtgc gcgacgtcct
      781 ggccacccag tgcctggcca tggccaagcc gaagctccgc caggtccggg tggacggcgc
      841 gctgtccaag ggcgtctacg ccaaggatgt catcctcgcg atcatccgca ccctgggcgt
      901 caacgggggc gtgggctacg cctacgagta cgcgggggcg gtggtggagc ggatgacgat
      961 ggaggagcgg ctcacgctct gcaacatgtc catcgagggc ggcgcgcgct ccggctacgt
     1021 caacccggac cagatcacct tcgactacct gcgcggccgc cagtacgccc cgcagggcgc
     1081 ggcgttcgat cgcgcggtga cgtggtggaa gggcatggcg tccgacgcga atgccgccta
     1141 cgacgacgtc aggcggctcg acggcaacgc catcccgccg atggtgacct ggggcatcaa
     1201 cccgggccag gcgatcggcg tgggcgagcg gatcccgtcc cccgagtcgg cgccggagcc
     1261 ggagcgcgcg acctaccggg agggcctggc ccacatgggc ttcgaggccg gccagcccat
     1321 cgcgggcgcc aagatcgacg tggtgttcat cggctcctgc accaactccc ggatctcgga
     1381 cctgcgcgtc gccgccgcgg tggccaaggg caaccgcgtg gccaagggcg tgcgggcgat
     1441 cgtggtgccg ggctcgcaga tggtggccaa ggtcgcggag gccgaggggc tccacgaggt
     1501 tttccgcgag gcgggcttcg agtggcggaa ggcgggctgc tcgatgtgcc tcggcatgaa
     1561 cgacgacaag ctccggggcc gcgagatgag tgcgtccacc agcaaccgca acttcatcgg
     1621 gcgccagggc agccccaccg gccgcaccct gctgatgagc ccggccatgg ccgcggcggc
     1681 cgcgctcaac ggcgcggtca ccgacgtgcg ggagatgatg cgatgagcga cagcgcgcgg
     1741 cggcagatcg cgggacgcgg catcccgatc cccggcaacg acatcgacac cgatcggatc
     1801 atcccggcgc ggttcctcaa gacggtcacc aacgagggcc tggggctgca cgccttcgag
     1861 gacgcgcgca agcacgaccc ggagcacccg ttcaaccaga aggcctacga aggcgcctcg
     1921 gtgctggtgg cgggcatgaa cttcggctgc gggtcttcgc gtgagcacgc tccggaatcg
     1981 ctcaagcagt ggggcatcaa ggccatcgtg ggcggctcct tcggcgagat cttcttcggc
     2041 aactgcacca tgctcggcat tccgtgcctg tcgatctccc aggacgacgt gctgtggctg
     2101 cagcgcgcgg tcggccgcga tccgaagcag gcggtcacgg tggacgtgga gcggcaggag
     2161 gtgcgcgtgg gcgatcgggt catcccggcc cggattcccg acggcgcgcg caaccagctc
     2221 gtctccggcg cgtggaacgc caccggcgtg ctgctggacg cgggcgaggc gatcgaggcc
     2281 accgcgggtc gcctgccgta cgtcaagggc ttctgatccg ggccggcgcg aaggtgcgga
     2341 ccgtccgcac gccgaccctc gagatcggct acgaggagtg gggggaggcg ggaggcgcgc
     2401 cggtcgtcct cctccacggc ttccccgacg acgcgcacgc ctgggacggg gtggcgccgg
     2461 cgctggccgc ccgcggctgc cgggtgctcg cgccctacct gcgcggctat gggcccacgc
     2521 gcttcctcga cgcgggcgcg ccgcgcctgg cgcagcaggc cgcgatcggc caggacctgc
     2581 tggacttcct ggacgcgctc gggatcgagc gggccgggct ggccggctac gactggggtg
     2641 ggcgcgcggc ctgcatcacg gccatcctgg cgcccgcacg ggtgcgcgcc ctggtcacca
     2701 tcggcggcta caacgtccag aacaccatcg cgccaccggt gcccgcgtcc gcgctgcagg
     2761 agcgggccaa ctggtaccag tggtacttca acaccgagcg gggacggctc gggctcgagc
     2821 agaaccgccg cgagatctgc cggctgctct ggcgggactg gtcgccgggc tggcgcttcg
     2881 acgacgcgac gttcgagctg gcggcggccg ccttcgacaa tcccgacttc gttccggtgg
     2941 tgatccactc ctaccggcac cgccaccgca acgcgccggg cgagccgcgc ttcgacgcga
     3001 tcgagcgccg cctcgccggg cggccgcgca tcgaggtgcc gagcgtgatc ctgcacggcc
     3061 gcgacgacgg cgtggacccc ccggcgcgca gcgagaagca ccccgccctg ttcccggccg
     3121 ggaccgaacg gtacgtgatc ccggacgccg gccacttcct cccgcgcgag cagcccggcg
     3181 cggtggtgga cgcgctgctc cccctgctgg cccggacggc gcggtaggcg atgtggttcg
     3241 tgctcgcggt gctcgcggcc ctgtgccagg tgctgcgcaa cacggtgatg aagcgcctcg
     3301 gccactcgct cgacgagtac atcaacgtgt ggggacgatt cacgttcctg ctgcccttcg
     3361 ccggcgcctt cgtgctgtgg cacggggtgc ccgagctcaa gcccgggttc tatctcgcct
     3421 gcgcctcgtt cgcggtctgc cagaccatct cgaccatggc gctctcgaag gccctcaagc
     3481 tcgccgagat ctccatggtc accgccctgt ggaaggtgag cctgctgatc ctggtcgtgc
     3541 tgggctactt cacgctgcgg gagcagccga ccctcctcgg catcgcgggc atcctgatca
     3601 gcatgtccgg cgtctacctg ctgaacgtca accgggccca catcgcctgg tgggcgccgc
     3661 tggccgtgct cttcacggac cgggggatgc gctacacgct gctggccgcg ctcttcttcg
     3721 cgccgtcggt ggtcaccatc aagtgggcca tgcagtgctc cgaccagtac atgggcaccc
     3781 tgggcggcta cgtggccgcg agcctgctcg tgaccccgat cgtgctcggg acctcccgcc
     3841 gccgcttcgt cgaggtgccg cgctactgga aggccttcgt gagcttcggg ctcttcgcct
     3901 cgctcacgac catcagccag ggcaccgcct atctgatgac gctgtcctcc tacgtggagg
     3961 ccgtgaagca ggtggagatc ctcttcgcga tggcgatcgg caccgtgatg ttccacgaga
     4021 agcagaaggt gcgggacatc gcgccggggg cgctggtgat gctggcgggg gtggtgctgc
     4081 tgagcctggc cggctagatc tccggccgct tgccgtgggt ggcgcggacc accgtcttga
     4141 tgccgccgcg caccgtgaag tctccgatca cctcgatcca gcgcggcgcc gccgcggcca
     4201 cgaaatcgtt caggatccgg ttggtcacgt cctcgtggaa ggccccttcg ttccggtagg
     4261 accagatgta gagcttgatc gccttcagct cgaggaggtg ccggtccggc acgtatcgca
     4321 cccgaatggt cgcgaagtcg ggctggccgg tgcgggggca gacgcaggtg aactcgggga
     4381 tctcgaggct gatctcgtag tcgcggtccg gatgtggatt gggcacgacc tcgagcgcct
     4441 tcgacggctg ggtgggcatg gcccgacgct accgcagggc gctcggggcg gtcaaggcgt
     4501 gcccctcacc ctgccctctc cccggagggg aga
//
LOCUS       DASUFA010000310         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_81756, whole genome shotgun sequence.
ACCESSION   DASUFA010000310 DASUFA010000000
VERSION     DASUFA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81756"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VFX87_11590"
     CDS             <1..1239
                     /locus_tag="VFX87_11590"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX4995610.1"
                     /translation="TWGEVTVRYEPIGRAPSAGGGSPVGARGEIRVEPSRHLTGKLLL
                     QPLEPGTRYRYTAVQNEEEATGEFVTAPAPADARPVRFSWSGDLGSRGYCRKPGDGYA
                     IFRALAQVPSDFFLFVGDTIYADHACGEAAHAPGYDFVARRLADFWAKHRYNREDPAV
                     QAYFRRTSVYAIWDDHEVRDNFSGPTEPLMEPGRRAFIDYFPIRPPREEPGRLYRQFR
                     WGALLEVFILDTRQYRSPNTAVDGPGKTMLGATQRRWLLDAVTGSTAVWKVVVSSVPL
                     SVPTGGRAHDSWSNATARGVPEEHGTGFAVERDGILRTLRQRGVKNLVVLAADVHHAE
                     LIRHHPTPEWSFHEFIAGPLSAGPGKPHPLDAGLTPRSLWSLGGTANFGDIAIDATGL
                     TVRIVDALGQTRFSHTVGAE"
     gene            complement(1241..>2173)
                     /locus_tag="VFX87_11595"
     CDS             complement(1241..>2173)
                     /locus_tag="VFX87_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4995611.1"
                     /translation="ARGLVAAGSARVSGSGRLGALPVALLLLCCLVWGVNQVAIKVSV
                     AGVSPLLGAGLRSVVAGALVWLWCWARGEPLFRRDGTGVYGLLIGLMFAGEFALIYWG
                     LAYTTASRSVIFLYSAPFFVALGAHRYIPGERLTRRRTAGLVLAFAGLCLACADALYL
                     PSRRELFGDMLELAAGLVWGATTVLLKKGVMLEQGGRVPLTPPRMLFYQLAVSSVALV
                     GASLALGEPGVVAPTAPVLAALAYQAVIVAFASYLAWFWLLRRYPASDLAPYLFWTPL
                     FGVLAGWVLLGDPLSGNLGGAALLIALGIYLVNR"
BASE COUNT          346 a          822 c          763 g          242 t
ORIGIN      
        1 acctggggcg aggtcaccgt gcgctacgag cccatcggcc gcgcgccgag cgccgggggt
       61 ggctcgcccg tgggtgcccg cggcgagatc cgggtggagc cgagccgcca tctgaccggc
      121 aagctcctgc tccagccgct cgagccgggc acgcgctacc gctacaccgc ggtccagaac
      181 gaggaggagg ccaccgggga gttcgtgacc gcccccgcgc cggccgacgc gcggccggtg
      241 cgcttctcct ggagcggcga tctgggcagc cgcgggtact gccgcaagcc gggcgacggc
      301 tacgcgatct tccgcgccct cgcgcaagta ccctccgatt tctttctctt cgtcggcgac
      361 accatctacg ccgaccacgc gtgcggcgag gccgcgcacg ccccgggcta cgacttcgtg
      421 gcgcggcggc tggccgactt ctgggccaag catcgctaca atcgcgagga cccggcggtg
      481 caggcctact tccggcgcac gtcggtgtac gcgatctggg acgatcacga ggtccgtgac
      541 aacttctccg ggcccaccga gcccctgatg gagccggggc ggcgcgcctt catcgactac
      601 ttcccgatcc ggccgccgcg cgaggagccc ggccgcctct accgccagtt ccgctggggc
      661 gcgctgctcg aggtgttcat cctcgacacg cggcagtacc gcagccccaa taccgcggtg
      721 gacgggcccg gcaagaccat gctcggggcc acgcagcgcc gctggctgct ggacgcggtg
      781 accggctcca ccgcggtgtg gaaggtcgtg gtatcgagcg tacccctctc ggtgcccacc
      841 ggcggtcgcg cccacgactc ctggagcaat gccaccgccc ggggcgtgcc ggaggagcac
      901 ggcaccggct tcgcggtcga gcgcgacggc atcctgcgca ccctgcgcca gcggggcgtg
      961 aagaacctgg tggtgctcgc cgccgacgtg caccacgccg agctgatccg ccaccacccg
     1021 acgcccgagt ggtcctttca cgagttcatc gcaggcccgc tctcggccgg ccccggcaag
     1081 ccgcacccgc tggacgccgg actcaccccg cgctcgctgt ggagcctggg cggcaccgcc
     1141 aacttcggcg acatcgcgat cgacgccacc ggcctcacgg tgcgcatcgt ggacgcgctc
     1201 ggccagaccc gcttctccca caccgtcggc gccgagtagc tcagcggttc accaggtaga
     1261 ttcccagcgc gatgagcagc gcggcgccgc ccagattgcc gctcagcgga tcgcccagca
     1321 gcacccagcc cgcgagcacg ccgaagagcg gcgtccagaa gagatacgga gcgaggtcgg
     1381 aggcggggta gcgcctgagc agccagaacc acgccagata ggacgcgaac gcgacgatca
     1441 ccgcctggta ggcgagcgcg gccagcaccg gcgcggtcgg cgcgacgacg cccggctcgc
     1501 ccagcgccag ggacgcgccg acgagcgcca cgctcgagac cgcgagctgg tagaagagca
     1561 tccgcggcgg ggtgagcggc acgcggccgc cctgctcgag catcacaccc ttcttgagca
     1621 ggaccgtggt ggccccccac acgaggccgg cggccagctc gagcatgtcg ccgaacagct
     1681 cgcgccggga aggcaggtac agcgcatccg cgcaggccag gcagaggccg gcgaaggcca
     1741 gcaccaggcc cgcggtgcgc cggcgggtca ggcgttcgcc cgggatgtag cggtgcgcgc
     1801 ccagggccac gaagaacggg gccgagtaga ggaagatcac gctgcgcgac gccgtggtgt
     1861 aggccaggcc ccagtagatg agcgcgaact cgcccgcgaa catgagcccg atgaggaggc
     1921 cgtagacccc ggtgccgtcg cggcggaaga gcggctcgcc gcgggcccag caccagagcc
     1981 agacgagcgc gccggcgacc actgagcgca gcccggcgcc caggagcggc gagacgccgg
     2041 ccacgctcac cttgatcgcc acctggttca ccccccagac caggcagcag agcagcagga
     2101 gcgcgaccgg cagtgcgccc agacggccgg acccgctcac gcgcgccgag cccgccgcca
     2161 ccaagccgcg agc
//
LOCUS       DASUFA010000311         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1205524, whole genome shotgun sequence.
ACCESSION   DASUFA010000311 DASUFA010000000
VERSION     DASUFA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205524"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..539)
                     /locus_tag="VFX87_11600"
     CDS             complement(21..539)
                     /locus_tag="VFX87_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4995612.1"
                     /translation="MLLPSRDGAPAAVEVTSGSARTRIDQPYDTARVRGEGPLEKGRS
                     SEAAVRETFGAALDAQPMRPVSFIVYFLEGKDELTPESLPVLGQIFAELARRPAPETV
                     VIGHTDALGSHASNDALSLQRAERMRDELVKRGVPRERIHVEGRGKRELLVPTADGIA
                     EPRNRRVEISVR"
     gene            complement(670..1131)
                     /locus_tag="VFX87_11605"
     CDS             complement(670..1131)
                     /locus_tag="VFX87_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX4995613.1"
                     /translation="MARWLLSSACLFFLVAGSPAASPALAADVGQIKVSKGTVSVQRE
                     GSTLAAPVGMGIRPSDVVVTGPDGSVGIVFADASLLSAGPGSVLAIERFVFDSTTHEG
                     AFETRLQKGTLSAVSGKIAKRTPEAMKIRTPSTVLAVRGTELAVRVGGPAE"
     gene            1283..2206
                     /locus_tag="VFX87_11610"
     CDS             1283..2206
                     /locus_tag="VFX87_11610"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX4995614.1"
                     /translation="MNQADRISGVLSPVVTPFKADLSPDPERFVRQCRWLLSQNVGLA
                     VFGTNSEANSLSVDEKIELLDRLVAAGLDPARMMPGTGACALTDTVRLTAHAVKAGCG
                     GVLMLPPFYYKGVSDDGLFRSFAEVIERVADLRLRVYVYHIPPVSQVPITLPLIERLL
                     KAYPGTVAGVKDSSGDWSNTKAMLDTFAPSGFDVFAGSETFLLANMRGGGKGCISATA
                     NVHPGPIARLFASWTGGDADAQQARLDEIRGVFQKFPMIPALKAAIAHHGGDPGWATV
                     RPPLVELAREPAAALVAELDRRGFTMPGLKA"
     gene            2229..2690
                     /locus_tag="VFX87_11615"
     CDS             2229..2690
                     /locus_tag="VFX87_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4995615.1"
                     /translation="MPVTGIYHTGLSVSDLDRSVSFYRDLLGLELIAQWDSSQPYLRT
                     VVGFPDGELRIALLRLPGEAGAAGHHLELLEYRVPRGARGDPRTCNPGNVHVAFRVED
                     LDATYRELRAKGVRFKSAPVEVTHGRNRGAKAVYLLDPDDITLEMMQPPRE"
     gene            2687..>3427
                     /locus_tag="VFX87_11620"
     CDS             2687..>3427
                     /locus_tag="VFX87_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4995616.1"
                     /translation="MTSLDLFRLEGQVAVVVGGAGGLGAAIARGLAEAGAAVAVADAN
                     AGQAKAIADELTRGGCRALAVAVDVSDGESVERMAGEVESRLGPIEVLVNSAGITHRS
                     PATDFPEAQWHRVIAVNLTGVFLASQAVGRRMVGRRRGRIINIASIAGEIGLPGTIAY
                     SASKGGVVQLTRALAVEWAPYDVRVNAIAPSWFSTNIGDLIHREPDYAERAMRRVPVG
                     RMGQPEELVGAALYLASEASRMVTGHILA"
BASE COUNT          478 a         1235 c         1233 g          481 t
ORIGIN      
        1 acgggcgtcg gaggctcgcg tcagcggacg ctgatctcca cgcgccggtt gcgcggctcg
       61 gcgatcccgt ccgcggtggg caccagcagc tcgcgcttgc cgcggccctc gacgtggatg
      121 cgctcgcggg gcacgccccg cttgaccagc tcgtcgcgca tccgctcggc gcgctgaagc
      181 gagagcgcgt cgttggaggc atggctcccc agcgcgtcgg tgtgaccgat caccacggtc
      241 tccggggcgg gacgccgggc aagctcggcg aagatctggc cgagcaccgg gagcgactcc
      301 ggcgtgagct cgtccttgcc ctcgaggaaa tacacgatga aggacaccgg acgcatcggc
      361 tgggcgtcga gcgcggcgcc gaacgtctcg cgcaccgcgg cctcgctcga tcggcccttc
      421 tcgagcggcc cttcgccccg gacgcgggcg gtgtcatacg gttggtcgat cctggtccgc
      481 gcggagccgg aggtcacctc caccgcggcg ggagcgccgt ctcgtgaggg gaggagcacg
      541 acaagctcct cgcgaacggg ccgggcgggg ctgacggggc gggcgcaggc gaccgccagc
      601 ggcacgccga gcgcgagcag accgagagcg agcgccccgg accgcttcct caaccggatc
      661 gcgccgcgct cactcggcgg gcccgccgac ccgcaccgcg agctcggtgc cgcgcacggc
      721 cagcacggtg gagggcgtcc ggatcttcat ggcctcggga gtgcgcttgg cgatcttgcc
      781 cgagaccgcg gagagcgtcc ccttctggag ccgcgtctcg aaggcgccct cgtgcgtggt
      841 ggagtcgaag acgaagcgct cgatcgcgag cacgctgccg gggcccgccg agagcaggct
      901 ggcgtcggcg aaaacgatgc cgacggaccc gtcgggcccg gtcaccacca cgtcggacgg
      961 ccggatgccc atccccaccg gggcggccag ggtgctcccc tcacgctgga cgctgacggt
     1021 ccccttcgag accttgatct ggccgacatc ggcggcgagc gccgggctcg cggccggaga
     1081 tccagcgacc aggaaaaaca gacaagcgct cgagagcagc caacgcgcca tgggcgttcc
     1141 tttgttcggt ccggtgtcgc ccggctcggc cgggcgcgga ccctatcata gcccggcggg
     1201 gccggcggcg ccccttgacc gtgggggcac gggaagacta ggctcatcgg gtcaccatca
     1261 tcgacggcac aggagcccca gcgtgaatca agcggaccgc atttccggcg tgctctcccc
     1321 cgtggtcacc ccgttcaagg ccgacttgag ccccgatccc gagcgcttcg tgcgccagtg
     1381 ccgctggctc ctgtcgcaga acgtgggcct ggccgtgttc gggaccaact ccgaggcgaa
     1441 ctcgctgtcg gtggacgaga agatcgagct gctcgaccgc ctcgtcgccg ccgggctcga
     1501 tcccgcgcgc atgatgccgg gcacgggtgc ctgcgctctc accgacacgg tgcggctcac
     1561 cgcgcacgcg gtgaaggcgg gctgcggcgg cgtgctcatg ctgccgccct tctactacaa
     1621 gggcgtgagc gacgacgggc tcttccggag cttcgcggag gtgatcgagc gggtggccga
     1681 cctgcggctg cgcgtgtacg tctatcacat cccgccggtc tcgcaggtgc cgatcacgct
     1741 gcccctcatc gagcggctgc tcaaggccta tccggggacg gtggcgggcg tgaaggactc
     1801 ctccggcgac tggagcaata ccaaggccat gctggacacc ttcgccccct ccgggttcga
     1861 cgtcttcgcg ggcagcgaga ccttcctcct cgcgaatatg cggggcggcg gcaagggctg
     1921 catcagcgcc accgccaacg tgcatccggg gcccatcgcg cggctcttcg cctcctggac
     1981 gggcggggac gccgacgcgc agcaggcgcg gctggacgag atccgcggcg tcttccagaa
     2041 gttccccatg atccccgcgc tgaaggcggc catcgcgcac catggcggcg accccggttg
     2101 ggccaccgtg cgcccgcccc tggtggagct cgcccgcgag cccgccgccg cgctggtcgc
     2161 ggagctcgat cgtcgcggct tcaccatgcc cggcctcaag gcctgaagcg cgggcccccg
     2221 gtcgtcacat gcccgtcacg gggatctacc acaccggtct cagcgtcagc gatctcgacc
     2281 gctccgtctc gttctaccgc gatctgctcg gcctcgagct gatcgcccag tgggacagca
     2341 gccagcccta tctccgcacc gtggtcggct tccccgacgg cgagctgcgc atcgccctcc
     2401 tccgcctgcc cggcgaggcg ggcgccgccg gccatcatct cgaattgctc gagtaccgcg
     2461 ttccgcgcgg ggcacggggc gacccgcgca cctgcaatcc cggaaacgtg cacgtggcct
     2521 tccgcgtgga ggatctggac gcgacctacc gtgagctccg ggccaagggc gtgcgcttca
     2581 aatcggcgcc ggtggaagtc acccatggac ggaaccgcgg cgccaaggcc gtgtacctcc
     2641 tggaccccga cgacatcacg ctcgagatga tgcagccgcc ccgcgaatga cgagcctcga
     2701 tctcttccgc ctggagggcc aggtggcggt ggtggtgggc ggcgcgggtg ggctcggcgc
     2761 cgccatcgcg cgcgggctgg ccgaggcggg cgcggcggtg gccgtcgcgg acgcgaacgc
     2821 cgggcaggcg aaggcgatcg ccgacgagct gacccgtggg ggctgccggg cgctggcggt
     2881 ggcggtggac gtgagcgacg gggagagcgt ggagcgcatg gccggcgagg tcgagtcgcg
     2941 cctcggcccc atcgaggtcc tcgtgaacag cgccggcatc acccaccgga gcccggcgac
     3001 ggatttcccc gaagcgcagt ggcatcgcgt gatcgcggtg aacctcaccg gcgtcttcct
     3061 ggccagccag gccgtcgggc gccggatggt gggccgccgc cggggccgca tcatcaatat
     3121 cgcctcgatc gcgggagaga tcgggctgcc gggcaccatc gcctactcgg cgagcaaggg
     3181 cggtgtcgtc cagctcacgc gcgcgctggc ggtggagtgg gctccgtacg atgtccgggt
     3241 caacgccatc gcgccgtcct ggttcagcac gaatatcggc gacctcatcc accgcgagcc
     3301 cgactacgcc gagcgcgcca tgcgccgggt gccggtgggt cggatgggcc agccggagga
     3361 gctggtgggg gcggcgctct atctcgcctc ggaggcctcc cgcatggtca ccggccacat
     3421 cctcgcc
//
LOCUS       DASUFA010000312         6297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_641565, whole genome shotgun sequence.
ACCESSION   DASUFA010000312 DASUFA010000000
VERSION     DASUFA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641565"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..528
                     /locus_tag="VFX87_11625"
     CDS             181..528
                     /locus_tag="VFX87_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4995617.1"
                     /translation="MTALMPAPETAPRPSPPRMPKGLSESSASPTAPARPLPGFHGAV
                     SDFKRRLIEATLTQAGGNRTRAARALGLQRTYLLRLIREFEVRVPPAPLPARRAELTA
                     PSREARAAPAQTR"
     gene            561..1688
                     /locus_tag="VFX87_11630"
     CDS             561..1688
                     /locus_tag="VFX87_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995618.1"
                     /translation="MSDVWIAGAGMTRFGRRDENLQDLMAEAGLAAMHDAGIERPDAL
                     VVAAMNPEEFTGEGNYGSLIATRLGLSYVPALRVETATSSGVAAVYAAYAAIAAGFHR
                     SVLVVGGEKMTHLPTPRVSEIIGRSIDPHERSYGTTMPALAGLITRAVMHRRGLSLRE
                     ISQVAVKNHAHGARNPLAHFQEAVTLEAVMESRLVADPLRLFHCCPISDGGAAVLLTA
                     DRRAVRVAGIGQGADTIAVRYRTDLTAFRATRAAAEAAYRMAGFGAARVEVAEIHDAF
                     APFELISLEDTGLVPVGKAGRATLDGETAIGGRLPVNTSGGLKARGHPLAATGISQIV
                     ELFWQLTGRADGRQVEARVALAQSIGGLATNNWVTLLEARR"
     gene            1688..2116
                     /locus_tag="VFX87_11635"
     CDS             1688..2116
                     /locus_tag="VFX87_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX4995619.1"
                     /translation="MAERLRASRCPRCRRLVVPPASICPDHPVRMEGASLPPLGEVVS
                     FTTLHSPPEGFRAPLHLALVELEGGARLFCHGTATEGVKIGSHVSVEAVDSVFYFSHM
                     GFTDRARLFWRRAGASGERVAAIGKSVVKGMWNRRPRGED"
     gene            2103..3365
                     /locus_tag="VFX87_11640"
     CDS             2103..3365
                     /locus_tag="VFX87_11640"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4995620.1"
                     /translation="MAKTKPRSRKTAGAARPKPAARRTSARPPLAGVRVIDLTRVLAG
                     PFCAMSLGDMGAEVIKIEEPGKGDDTRGWPPFVSGEATYFLSVNRNKKSLTLNMKAPE
                     GQEILRKLLASADVVLENFRPGTMERLGFGYEALRKVNPRLVYCSISGFGESGPEAHR
                     PGYDLIVQGESGVMDITGFPDGPPVKVGNSIGDLVAGMAAAQGITLALLSREKTGRGQ
                     KVEIGMLDVMASLLTYPAGLYWNAGGRPARRGNEHPSIVPYEVFKARDVYITLGVANN
                     SLWDRTCRALEREDLIKDPRFDTEANRVTNRKVLVPLLNEILGARPADEWLARLDKAG
                     VPAGRIKSVAEVCESAHLKARGMMVSLSHPKAGPITVMGVPVRLHDTPGAASLAPPLL
                     GQHTEAILTGLLRMPKARVEKLRAAGVV"
     gene            3370..3795
                     /locus_tag="VFX87_11645"
     CDS             3370..3795
                     /locus_tag="VFX87_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4995621.1"
                     /translation="MTAKIGPLGLEVGQRARRTQTVTSQDVELYARLTGDRNPLHFDA
                     DFAARTRFGRLVAQGGIAAGMLNALVAMDLPGPGTVFMSQSLKYLAPTYLGDTVTAEI
                     EVVSLKPDKPVCQLHAVITNQDGVTLLEGECWTYTMRPA"
     gene            complement(3806..4549)
                     /locus_tag="VFX87_11650"
     CDS             complement(3806..4549)
                     /locus_tag="VFX87_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4995622.1"
                     /translation="MLGAVIAALMAAVVLGAALVKGAIGFGFPALGTPLLSLVVDVKT
                     AVVVLILPNIVMDGIQFARRGTPLATVRRFAVLLAAGALGVMLGTRTLVVLSPRAATL
                     ILGVFVLCYVALNATGLAPRVPAHWERWLSAPAGFVAGVIGGITNAPATALVLYFQAI
                     GLSKHDFVSSVAFTFFFYKLAQLGAVSWYGLLPWSLLWPSLALTAVGLVGFAVGLRVQ
                     DRLDQRAFNRAVLGFLALLGAWLLIRSLW"
     gene            4592..6181
                     /gene="cimA"
                     /locus_tag="VFX87_11655"
     CDS             4592..6181
                     /gene="cimA"
                     /locus_tag="VFX87_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX4995623.1"
                     /translation="MSRQIKIYDTTLRDGTQGEGVAFSMEDKVRIAHRLDQLGVHYIE
                     GGWPGSNPKDLRFFKRVQDAVFKTAKISAFGATRRPGVRPQEDGNLQALVEAGPPVVT
                     IFGKAWDFHVTSALSTTLEENVAMISDSVAYLLKHFEEVIYDAEHFFDGYKRNPDYAM
                     RTLLAAEAAGAHCLVLCDTNGGCLPHEVAAVIKEIKGAVKAETPLGIHVHNDTECAVA
                     TSVAAVGEGIGHVQGTINGYGERCGNANLVSIIPNLMLKMGLECIPRQNLRELRDVSR
                     LVSELANRKPWSAQPYVGDSAFAHKGGIHVSAVLKHPETYEHVDPDAVGNQRRVLVSE
                     LAGQSNVLWKAKEYGIDLDKNTPEARRILEMLKRLEDEGFLFEGAEASFELLMERALG
                     NHRPYFELGGYRVIVEEDAGDGEPVAEATVRLRVKGIEEHTAAAGNGPVHALDHALRK
                     ALEDFYPNLREMALLDYKVRILDESKATAAKTRVLITSGDGNETWGTVGVADNIIEAS
                     WQALVDSVEYKLRRDEKQGKR"
BASE COUNT          969 a         2207 c         2276 g          845 t
ORIGIN      
        1 gccggccgtt cgcccgacga gggacccgtt ctcgggtccc tcttttcttt gccctgacgc
       61 gatggcaggg ccgcgcgcgg gctgcacacg gcgcggtgca cgggcgtgcg cggagcgggc
      121 ggattcgggg gttccgcgcg gcggaagcgg gtggcacagg ccttgcgata gggctcgcgc
      181 atgaccgcct tgatgcccgc tcccgagacc gctccccggc cgtcgccgcc ccgcatgccc
      241 aagggcctct ccgaatcgag cgcgtcgccg accgcgcccg cgcggccgct gcccggcttc
      301 cacggcgcgg tctccgactt caagcgccgg ctgatcgagg ccacgctcac gcaggcgggc
      361 ggcaaccgca cgcgcgcggc gcgggcgctc gggttgcagc gcacgtacct gctgcggctc
      421 atccgcgagt tcgaggtccg cgtgccgccg gcgccgctgc ccgcgcgccg cgctgagctg
      481 accgcgccct cgcgcgaggc gcgggctgcg ccggcgcaaa cacgttgaca cccggtcatg
      541 ccccggtttg aaatggatgg gtgagcgacg tgtggatcgc cggagcgggg atgacccgct
      601 tcggccggcg ggacgagaat ctccaggacc tcatggcgga ggcgggcctg gccgccatgc
      661 acgacgcggg catcgagcga cccgacgccc tcgtggttgc cgcgatgaac ccggaggagt
      721 tcaccggcga gggcaactac ggctcgctga tcgcgacacg gctcggcctt tcgtacgtgc
      781 ccgcgctgcg cgtggagact gcgacgtcct ccggggtggc cgcggtctac gcggcctacg
      841 cggcgatcgc ggccgggttc catcgctccg tcctcgtggt gggcggcgag aagatgaccc
      901 acctgccgac gccgcgcgtg tccgagatca tcggccgctc catcgacccg cacgagagga
      961 gctacggcac caccatgccc gccctcgccg gcctcatcac gcgcgcggtc atgcaccggc
     1021 gcgggctgtc gctgcgggag atctcgcagg tggcggtgaa gaaccacgcc cacggtgccc
     1081 gcaaccccct ggcccacttc caggaggcgg tcacgctcga ggcggtgatg gagagccggc
     1141 tcgtggccga tccgctgcgc ctcttccact gctgccccat ctcggacggc ggcgccgcgg
     1201 tcctgctgac cgcggaccgg cgggccgtgc gagtggcggg gatcgggcag ggggcggaca
     1261 ccatcgcggt gcgctaccgg acggacctga ccgcgttccg cgcgacccgg gccgcggcgg
     1321 aggcggccta tcgcatggcc ggcttcgggg cggcgcgggt ggaggtggcg gagatccacg
     1381 acgccttcgc cccgttcgag ctgatctcgc tcgaggacac cgggctggtg ccggtgggca
     1441 aggcgggacg ggccacgctc gacggagaaa ccgccatcgg ggggcggttg cccgtgaaca
     1501 cctccggcgg gctcaaggcg cgcggccacc cgctcgccgc caccggcatc tcgcagatcg
     1561 tggagctctt ctggcagctc accgggcggg ccgacggccg gcaggtggag gcgcgcgtgg
     1621 cgctggccca gtccatcggc gggctcgcca ccaacaactg ggtgaccctg ctggaggcgc
     1681 gccgctgatg gccgagcggc tccgcgcctc ccgctgcccg cgctgccggc gcctcgtggt
     1741 gccgccggcc agcatctgtc ccgatcatcc ggtgcggatg gagggcgcct cgctgccgcc
     1801 gctcggggag gtggtctcgt tcaccacgct ccattcgccg cccgagggct tccgggcgcc
     1861 gctccacctg gcgctggtcg agctcgaggg cggggcccgg ctcttctgcc acggtaccgc
     1921 caccgagggc gtgaagatcg gctcccacgt gtcggtggaa gcggtagact ccgtgttcta
     1981 cttctctcac atgggattca cggaccgggc gcggctcttc tggcgccgcg ccggcgccag
     2041 tggcgagcgc gtcgccgcga tcggcaagag cgtcgtcaaa ggcatgtgga acaggaggcc
     2101 ccgtggcgaa gactaagccc cgctcccgca agaccgccgg cgccgcgcgt ccgaagcccg
     2161 cggcccgccg gacgtccgcc cggccgccgc tcgccggcgt gcgcgtcatc gacctcaccc
     2221 gcgtgctcgc cggcccgttc tgcgccatgt cgctgggcga catgggtgcg gaggtgatca
     2281 agatcgagga gcccggcaag ggcgacgaca cccgcggctg gccgcccttc gtgagcggcg
     2341 aggccaccta cttcctctcg gtcaaccgca acaagaagag cctcacgctg aacatgaagg
     2401 cgcccgaggg acaggagatc ctccgcaagc tgctggcctc ggccgacgtg gtgctggaga
     2461 acttccggcc cggcaccatg gagcgtctcg gcttcggcta cgaggcgctc agaaaggtca
     2521 atcccaggct cgtctactgc tcgatctcgg gcttcggcga gagcggcccc gaggcgcacc
     2581 ggcccggtta cgacctgatc gtgcagggcg agtccggcgt catggacatc acgggattcc
     2641 ccgacggccc gccggtcaag gtgggcaact cgatcggcga cctggtggcg gggatggccg
     2701 cggcgcaggg catcacgctg gcgctgctga gccgcgagaa gaccggccgc gggcagaagg
     2761 tcgagatcgg catgctcgac gtgatggcct cgctgctcac ctacccggcc gggctctact
     2821 ggaacgcggg cggccggccc gcgcgacggg gcaacgagca cccgtccatc gtgccgtacg
     2881 aggtcttcaa ggcgcgggac gtgtacatca cgctgggggt ggcgaacaac tccctgtggg
     2941 atcgcacctg ccgggcgctg gagcgcgagg atctcatcaa ggatccgcgc ttcgacaccg
     3001 aggccaatcg cgtgaccaac cggaaggtgc tggtgccgct cctcaacgag atcctgggcg
     3061 cacgccccgc ggacgagtgg ctcgcgcggc tggacaaggc gggcgtgccc gccgggcgca
     3121 tcaagtcggt ggccgaggtg tgcgagagcg cgcatctcaa ggcgcggggc atgatggtct
     3181 cgctgtcgca tccgaaggcg gggccgatca ccgtgatggg cgtgccggtg cgcctgcacg
     3241 acacgccggg cgcggcttca ctggccccgc cgctgctcgg ccagcacacc gaggcgatcc
     3301 tgaccgggct cctccgcatg ccgaaggcgc gggtggagaa gctgcgcgcc gccggcgtcg
     3361 tgtagcatct tgacggcaaa gatcggcccg ctcggcctcg aggtcggcca gcgcgcgcgc
     3421 cgcacccaga ccgtgacttc tcaggacgtt gagctctacg cgcggctcac cggcgatcgc
     3481 aacccgctcc acttcgacgc cgacttcgcc gcgcggaccc gcttcggtcg cctggtcgcg
     3541 cagggcggca tcgcggcggg gatgctcaac gcgctggtcg ccatggacct gccgggcccg
     3601 ggcaccgtgt tcatgagcca gtcgctcaag tacctggcgc cgacctatct cggcgacacg
     3661 gtgaccgcgg agatcgaggt ggtgtcgctc aagcccgaca agcccgtctg ccagctgcac
     3721 gcggtgatca ccaaccagga cggcgtgacg ctgctggaag gcgaatgctg gacctacacc
     3781 atgcgcccgg cctgatccgg gtgtttcacc agaggctgcg gatcagcagc caggcgccca
     3841 gcagcgcgag gaatcccagc accgcgcggt tgaacgcgcg ctggtccagc cggtcctgca
     3901 cccgaaggcc gaccgcgaag ccgacgagcc ccaccgcggt cagggcgagc gagggccaga
     3961 gcagcgacca gggcaggagg ccgtaccagc tcaccgcgcc gagctgggcg agcttgtaga
     4021 agaagaacgt gaaggccacc gaggacacga agtcgtgctt cgagagcccg atggcctgga
     4081 agtagaggac gagcgcggtg gcgggcgcat tggtgatccc gccgatgacg ccggcgacga
     4141 agccggcggg cgccgagagc cagcgctccc agtgcgccgg cacccgcggg gccagccccg
     4201 tcgcgttgag cgcgacgtag cagagcacga agacgcccag gatcagcgtc gcggcgcggg
     4261 gtgagaggac gaccagcgtg cgcgtgccga gcatcacgcc gagggcgccc gccgcgagca
     4321 ggacggcgaa gcggcgcacg gtggcgagcg gggtgccgcg gcgggcgaac tggatgccgt
     4381 ccatcacgat gttgggcagg atcaggacga cgaccgcggt cttgacgtcc accaccaggc
     4441 tcagcagggg cgtgccgagg gcggggaagc cgaagccgat ggcgcccttg accaaggctg
     4501 ctcccagcac caccgccgcc atcagggccg cgatcacggc cccgagcata acttgacacc
     4561 ccccggccgg cggcgggatg atgtgccccc tatgtcgcgc cagatcaaga tttacgacac
     4621 cacgctccgc gacggcacgc agggagaggg cgtggccttc tccatggagg acaaggtgcg
     4681 catcgcgcac cgcctcgatc agctcggcgt ccactacatc gagggcggct ggcccgggtc
     4741 caatcccaag gacctgcgct tcttcaagcg cgtgcaggac gcggtgttca agaccgcgaa
     4801 gatcagcgct ttcggcgcca cccggcggcc cggcgtccgg ccgcaggagg acggcaacct
     4861 ccaggcgctc gtcgaggcgg gcccgcccgt cgtcaccatc ttcggcaagg cgtgggactt
     4921 ccacgtgacc tcggcgctct ccaccacgct cgaggagaac gtggcgatga tctcggattc
     4981 ggtggcctac ctcctcaagc acttcgagga ggtcatctac gacgccgagc acttcttcga
     5041 cggctacaag cgcaacccgg actacgcgat gcgcacgctg ctcgccgcgg aggccgcggg
     5101 cgcccactgc ctcgtgctct gcgacaccaa cggcgggtgc ctgccgcacg aagtggccgc
     5161 cgtgatcaag gagatcaagg gcgcggtgaa ggcggagact ccgctcggca tccacgtgca
     5221 caacgacacc gagtgcgcgg tggcgacgtc ggtggccgcg gtgggtgagg gcatcggcca
     5281 cgtgcagggg acgatcaacg gctacggcga gcgctgcggc aacgccaacc tcgtctcgat
     5341 catcccgaac ctcatgctga agatggggct cgagtgcatc ccgcggcaga atctgcgcga
     5401 gctccgcgac gtctcccggc tcgtctccga gctggccaac cgcaagccgt ggtcggccca
     5461 gccctacgtg ggcgactccg cgttcgccca caagggcggc atccacgtct ccgcggtgct
     5521 caagcacccc gagacctacg agcacgtgga cccggacgcg gtgggcaacc agcggcgcgt
     5581 gctcgtctcc gagctggccg ggcagtccaa cgtgctctgg aaggccaagg agtacggaat
     5641 cgacctggac aagaacaccc cggaggcccg ccgcatcctc gagatgctca agcggctcga
     5701 ggacgagggc ttcctcttcg agggcgcgga ggcctccttc gagctcctca tggagcgcgc
     5761 tctcggcaac caccggccgt acttcgagct cgggggctat cgcgtgatcg tggaggagga
     5821 cgcgggggac ggggagccgg tggcggaagc gaccgtgcgc ctgcgcgtga agggcatcga
     5881 ggagcacacc gcggcggcgg gcaacgggcc ggtgcacgcg ctcgaccacg ccctccgcaa
     5941 ggccctcgag gacttctacc cgaacctgcg cgagatggcg ctgctcgact acaaggtgcg
     6001 gatcctcgac gagtcgaagg ccaccgcggc caagacccgc gtgctcatca cgtccggcga
     6061 cgggaacgag acgtggggga cggtcggggt cgcggacaat atcatcgagg cgtcctggca
     6121 ggcgctcgtc gactccgtgg agtacaagct gcggcgagac gagaaacagg gtaagcggta
     6181 gggccgggag cgggcggccg gcgggggcgg gacggggtct ctgaccacga cgcgcttcct
     6241 cgtctcgggc tggttgcagt tcccaatcgg tgacatgagg ctcctggctt cccgcgg
//
LOCUS       DASUFA010000313        12209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1115406, whole genome shotgun sequence.
ACCESSION   DASUFA010000313 DASUFA010000000
VERSION     DASUFA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115406"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFX87_11660"
     CDS             <1..961
                     /locus_tag="VFX87_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX4995624.1"
                     /translation="RRQPGSAFKPFVFLAALTPRKDASLFTPASLVEDAPITVMVDGK
                     PWTPKNYDDRYEGPVTVRRALEGSLNTATVRVAQAVGLPVVIETARTLGMEVDLKPVP
                     ALTLGVFEITPLALARAYLPLANGGLVPAGGGVVETVADEGGRALWSASREARPVVGA
                     PEAYLVTSLLQGVINVGTAASARAAGVPGAVAGKTGTTNEGRDAWFVGYSPNLLALVW
                     VGFDDGTPAGLSGSEGALPIWSEFMRQALDIYPGGVFPEPAGITHAKVDVTTGRLATA
                     FCPLVATEVFLAGSEPPPCEEHGGSVTEHIERWWDKVRGWFGR"
     gene            complement(978..1292)
                     /locus_tag="VFX87_11665"
     CDS             complement(978..1292)
                     /locus_tag="VFX87_11665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4995625.1"
                     /translation="MITTKHAADRRRHPRTDVSWLVVVEAGKRRFLLQTVDISARGAK
                     VRPKERIDIGTPAQLRFRPPDGSPFNVPALVWRQDSDGLAFLFMGDIQDRLRRFGRVL
                     AL"
     gene            1441..2817
                     /locus_tag="VFX87_11670"
     CDS             1441..2817
                     /locus_tag="VFX87_11670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4995626.1"
                     /translation="MSAATAEVVICGAGMAGVAAAYHLAVRRGLGGIALVDERPALSL
                     TSDKSAESYRNWWPGPGDAMVALMNRSIDLMEELARESGNVFLMNRRGYLFATAQPEQ
                     IDRYVRAAEEAAGFGAGPVRMHRSSADTYQPAPAVGYEKQPVGTDVITDPSLIRRHFP
                     YLAPDTVALLHARRCGWFSGQQLGMYMLERAREKGVRLIEGRVERVETAGGRVNGVAV
                     SGRDGTRTIGTPRFVNAAGPFVKDVGRLLGVELPVFCERHAKVAFHDTLGVVPRHAPM
                     MIWTDPVRLPWSDDERAELGEADSLRHLLGEMPAGVHGRPEGGGDSPVMLGVWTYHAE
                     PMEARFPVEFDPAYAEIVLRGLTRPLPGLAAYLSRFPKCFVDGGYYTKTQENRFLSCP
                     LPIQGAYVLGGLSGYGMMSSNGAADLLADYIAERPLPHYAPAFHLDRYQDPAYRALLE
                     NWGDSGQL"
     gene            complement(2825..4114)
                     /locus_tag="VFX87_11675"
     CDS             complement(2825..4114)
                     /locus_tag="VFX87_11675"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEX4995627.1"
                     /translation="MKGVTLPTETVFVMENSPIKFGLGAMDEIGFDASRLGIRKALIF
                     TDRHISELGLSERVRALLEEQGVKADVWDGVEVEPTDRSMEEAGEYARSKDFDGLIAV
                     GGGSTIDTCKAANLLTCYPAPLLDYINKPVGRGVPVPGPVRPLIAVPTTAGTGSETTA
                     VAVTHIVDQNVKAGVSHRLLRPALGVVDPLNTLTAPPEVTAAAGADILTHAIESYTTR
                     PYNARAKHHPPDRPAYIGANPASDIWCEKAMEYAARYLRRAVLNGLDVEARLHLALAA
                     NYAGIGFGNAGVHIPHAVAYPIAGLVRDYIPSGYRTRHPLVPHGMSVILTAPAAFRFT
                     YTTAPERHLRAAELLGWPVADLNEAERREALPQALLSLMRDIGIPNGLTAIGYTERDL
                     PALIEGTLRQPRLLSGAPRPVGAAELDLILRDAMCYW"
     gene            4244..5770
                     /locus_tag="VFX87_11680"
     CDS             4244..5770
                     /locus_tag="VFX87_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995628.1"
                     /translation="MPLFAWIAALLLTIGASPAHAQPGQITIVQPAEATTMDPGRSTQ
                     VLTVNYFYNLYDSLLRWDTALQLRPGLATAWRAVNDTTWEFTLRSGVKFHDGSPLTAD
                     DVRATLERNLIPGKTVVTAGFTTIESVQAVTPTLVRVITKKADPLLPVRMAQMGSQIL
                     PARLTTDDGVKELARRPVGSGAYRFVEWVKDERLVMEANRDWWGWEGRAPAIDRVVWK
                     AIPDDFPRLVALEKGDVDIVTSLPPDRIKSIAEGRNTRVVSVPSSRTVEFNINTTQPP
                     LSDKRVRQAMHYALDVPAVIRSLFAGLGKPMAGALADTDFGYNAAIKPYPYDPGRARA
                     LLGEAGFAGGIDVTLHAGMGTMVNDKQLLEVVADMWSKVGIRAKVAMMEMGARQRMVN
                     ERALPPGGLLLINVQSTLLDADGSLWRIMHPTGLGGKYWAGSQPGHRFHEVMEQARYA
                     LDPKTRKALYVEATQIIDDEKPWLELFQEVIVYGTSKRVAFRPRPDFRLIVSEMSLSR
                     "
     gene            complement(5844..6869)
                     /locus_tag="VFX87_11685"
     CDS             complement(5844..6869)
                     /locus_tag="VFX87_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX4995629.1"
                     /translation="MRLRSLMSFLADDLAVDLGTANTLVYVRGQGIVLNEPSIVAMKD
                     GVVLAAGHEAKAMLGRAPGSISVIRPLRHGVIADFDSTEKMLHYFIRKTHQRRSIVHP
                     RVVVTVPSGITQVEKRAVRQSAKQAGAREAYLIEEPIAAALGAGLPVAEPGANMIVDI
                     GGGTTEVAVMSLSGIVYCKSARIAGDEMNNAVIQHIRKNYSLLVGERHAEDIKLCLGS
                     AYPVAGEHLTMEVKGRDLIVGIPKTILVTGEEIREALREPIMAIVSAVHTCLEQIPPE
                     LAADIVDKGIVVSGGGALLRGLDLLLHEQTGLPIRVSDDPLTCVVRGAGALLDDVKLL
                     ARVALPS"
     gene            7112..7885
                     /locus_tag="VFX87_11690"
     CDS             7112..7885
                     /locus_tag="VFX87_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995630.1"
                     /translation="MKAPRKPPAKIEGRKRMQQSGLTVAWHGTRSRKDWAAYVENGSE
                     QGRLVLAEQVSERAVKSLLARIQSLSRDALEAMGRPAEAVAAQGGGPPVRRPRGGGEA
                     SSSSEIIVQPLPGRQLALSGDAEAPATTSPRRRRLPAKVERNAPAERRPARLSARDLA
                     GTVRQLRSRLESLEEEQDHLRAELALLRGDAEAYEGTPSIFVTGWFRATLVLIVLAIL
                     VVITVPWLMDIFEGGARESRPPIRNEAPAASPNPTPSGR"
     gene            complement(7888..8214)
                     /locus_tag="VFX87_11695"
     CDS             complement(7888..8214)
                     /locus_tag="VFX87_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995631.1"
                     /translation="MRKWIGALSLVWLLWSDLSVIVKDNPLDTIVLRWLARVPRLQTD
                     RIIAGRYASGDECKAALERHLVTVKAINDSWSGHTFEREGNMGTIHYHPAGIEYAHVA
                     LRCAEQ"
     gene            8359..8748
                     /locus_tag="VFX87_11700"
     CDS             8359..8748
                     /locus_tag="VFX87_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995632.1"
                     /translation="METILVVDDEAQVRTLARDILLGAGYRVLEAEDGEQALRVAEDH
                     PGAIHVLLTDIMMPGINGKELADRFLAARPDAKAIFMSGRAAEVISDAGVLIPVDAFL
                     AKPFTVERLLNKVRERLEYRSPFSRPR"
     gene            8798..9796
                     /locus_tag="VFX87_11705"
     CDS             8798..9796
                     /locus_tag="VFX87_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995633.1"
                     /translation="MTDGLPAVPPTAARVAAAVAASGRSRDELVAEMPPNFGRATVEK
                     IAVNAVMTGCRPEYFPVVLAAVEAMCDDRFCLLGVSGITDAVAPLVIVNGPIRQSLGI
                     NSGAGVFGPGWRANATIGRAVRLCWVNIGGAQPGVISMSTFGGPGRYTYCIGENEEAS
                     PWEPLHVEYGFAREESTVAVLAAEAPQIVADTRSRNAPDLLATIARSVEVVAHHKATG
                     LGDTALVFSPEHVTTLAADGWSKARARQFLWERLRKPVRELLPGRDGGEGLSDEALAA
                     FPAEGRSDALVPKFQAPESLKLLVAGGPAGRFTAIVPGWPFRNAPSRLVFRKIAPS"
     gene            complement(9793..10317)
                     /locus_tag="VFX87_11710"
     CDS             complement(9793..10317)
                     /locus_tag="VFX87_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4995634.1"
                     /translation="MSEFHDEYSESLDAEFRKLEDALLSDEVRLLAPSEPIRLPPDAS
                     VAEAVTRMVDNHRAGVVIVDPAGRLVGIFTERDLLTRVVRLGRELDRTRLAEVMTADP
                     EALTPDDRICFAINRMHSAGYRTVPLVDGDHRPIGVVTVNDVVGWLARVFPEAILNLR
                     PGDRANRPAQMDSG"
     gene            10658..10828
                     /locus_tag="VFX87_11715"
     CDS             10658..10828
                     /locus_tag="VFX87_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995635.1"
                     /translation="MKVICPRCQQEGKPSLLVTIDIPGDDREIHGLCWTHRLRLLAKS
                     TDVPPSREPGKR"
     gene            10858..11541
                     /locus_tag="VFX87_11720"
     CDS             10858..11541
                     /locus_tag="VFX87_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995636.1"
                     /translation="MSIWLWRTTPARRLLVIVGPGHPTLVPRIERLFEGEPGAVDVVL
                     DRRQRERRRVVMTRRPERRRRDRRRQHGAARRTGDVSVVIRRESAPGLDYTPPIQTKG
                     EGRMMSVESKQTRDGDDVRRWLEAGQTQLASLLELLHEHDRLRERVDSSERENERLRG
                     VTYENEQLRNRLETSERQAEHLRQSMGELRAENERHQKEREEAAERLNHLVNEIAQRL
                     RPGAGVSRT"
     gene            11692..12090
                     /locus_tag="VFX87_11725"
     CDS             11692..12090
                     /locus_tag="VFX87_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995637.1"
                     /translation="MTDTENARPRVLLVDDEEGVRMSLRALLEREYQIASVPSGEAAL
                     EELPRFRPDLVLLDVRLLPSMSGLDALERIKAFDERIEVVMITAFASLATLNEALRRG
                     ACGYLIKPFPRRDLELMVRDALARRGLRLN"
BASE COUNT         1831 a         4087 c         4462 g         1829 t
ORIGIN      
        1 ccgccgccag ccgggcagcg cgttcaagcc gttcgtgttc ctggccgcgc tcactccgcg
       61 gaaggacgcg agcctcttca cgcccgcctc gctggtcgag gacgcgccca tcacggtgat
      121 ggtggacggc aagccgtgga cgcccaagaa ctacgacgac cgctacgaag ggccggtcac
      181 cgtgcgccgg gcgctggagg gctcgctgaa caccgcaacg gtgcgggtcg cgcaggcggt
      241 ggggctgccg gtggtcatcg agacggcgcg cacgctgggc atggaggtgg atctcaaacc
      301 ggtgcccgcg ctcaccctcg gtgtcttcga gatcacgccg ctggcgcttg cgcgcgcgta
      361 cctgccgctg gccaacggcg gcctggtgcc ggccggcggc ggggtggtgg agacggttgc
      421 cgacgaaggc gggcgcgccc tctggagcgc gagccgcgag gcgcgcccgg tcgtcggcgc
      481 gccggaggcc tacctggtca cctcgctgct gcagggtgtg atcaatgtgg ggaccgcggc
      541 ctccgcgcgc gccgcgggcg tgcccggcgc ggtggccggc aagacgggga cgaccaacga
      601 ggggcgggac gcctggttcg tcggctactc gcccaacctg ctcgcgctgg tctgggtggg
      661 cttcgacgac ggcacgccgg ccgggctatc cgggtcggag ggcgccctgc cgatctggtc
      721 cgagttcatg cggcaagcgc tcgacatcta cccggggggc gtgttcccgg agccggcggg
      781 catcacacac gcgaaggtgg acgtgaccac cggccggctg gccaccgcgt tctgcccgct
      841 cgtggccacc gaggtgttcc tggccggcag cgagccccca ccttgcgagg agcacggcgg
      901 tagcgtcacg gagcacatcg agcgctggtg ggacaaggtc cggggctggt tcggaaggta
      961 gcgagtcgcg gccgcggcta gagcgcgagc acgcgaccga agcggcggag gcggtcctgg
     1021 atgtccccca tgaagaggaa ggcgaggccg tcggagtcct gtcgccacac gagtgccggc
     1081 acgttgaacg gcgacccgtc gggcggccgg aaccggagct gagccggcgt cccgatatcg
     1141 atccgctcct tgggccgcac cttggccccg cgggccgaga tgtccaccgt ctggagcagg
     1201 aagcgacgct tgccggcctc cacgacgacg agccaggaga cgtcggtgcg cgggtggcgg
     1261 cggcggtcgg cggcgtgttt tgtggtaatc atgccgcggg tacgagcaac ctgcgtgcct
     1321 cgtgcggacg cccgccatat cagggacttg gacccatgtc accaccgtgg cggaccgtca
     1381 agcccgtgtc gtgacgctga cagtgttcgc cggctgccaa agcgtgtaga gtcgggggcc
     1441 atgagcgcgg cgacggccga ggtggtgatc tgcggggcgg gcatggccgg tgtcgccgcc
     1501 gcgtatcacc tggcggtccg ccgcggtctc gggggaatcg ccctggtgga cgagcgcccc
     1561 gcgctctcgc tgaccagcga caagtccgcc gagtcttacc gcaactggtg gccggggccc
     1621 ggcgacgcga tggtggccct gatgaaccgc agcatcgacc tgatggaaga gctcgcgcgc
     1681 gagagcggca acgtcttcct gatgaaccgg cgcggctatc tcttcgccac cgcgcagccc
     1741 gagcagatcg accgctacgt gcgggccgcc gaggaagcgg ccggcttcgg ggccgggccg
     1801 gtccgcatgc atcgttcatc cgcggacacg taccagcccg cgcccgcggt cggctacgag
     1861 aagcagccgg tcgggaccga cgtcatcacc gatccgagtc tgatccgtcg ccacttcccg
     1921 tatctggcgc cggatacggt ggcgctgctg cacgcgcgac gctgcggctg gttcagcggc
     1981 cagcagctcg gcatgtacat gctcgagcgc gcgcgggaga agggcgttcg cctgatcgaa
     2041 gggcgcgtgg agcgcgtgga gaccgcgggc gggcgggtga acggagtggc ggtgagcggg
     2101 cgcgacggca cgcgcacgat cgggacgccg cgtttcgtga acgcggcggg acccttcgtg
     2161 aaggacgtgg ggcggctgct cggtgtcgag ctgcccgtct tctgcgagcg gcacgccaag
     2221 gtcgccttcc acgacacgct gggcgtggtg ccccggcacg cgccgatgat gatctggaca
     2281 gatccggtgc ggctgccgtg gtccgacgac gagcgcgcgg aactcggaga ggccgactcg
     2341 ctccggcacc tgctcgggga gatgcccgcc ggcgtgcacg ggcggcccga gggcggtggg
     2401 gacagcccgg tgatgcttgg cgtctggacc taccacgcgg agccgatgga ggcgcgcttc
     2461 ccggtcgagt tcgacccggc ctacgcggag atcgtgctgc gtggcctgac gcggccgctg
     2521 cccgggctcg ccgcttacct gtcacgcttc ccgaagtgct tcgtggacgg ggggtactac
     2581 accaagacgc aggagaaccg cttcctgtcc tgcccgctgc cgatccaggg cgcctacgtc
     2641 ctgggcggcc tctccggtta cggaatgatg tcgtccaacg gggccgcgga tctgctcgcc
     2701 gactacattg cggagcggcc gctaccccac tacgcgcccg cgttccacct ggaccgctat
     2761 caggaccccg cctaccgcgc cttgctcgag aactggggcg attccgggca gctctaggcc
     2821 ccgcctacca gtagcacatc gcgtcgcgga gaatcaggtc gagctcggcc gcgcccacgg
     2881 ggcggggagc gcccgagagg aggcgcggct ggcggagcgt gccctcgatc agcgccggga
     2941 gatcgcgctc ggtgtagccg atcgcggtga ggccgttggg gatgccgatg tcgcgcatga
     3001 gcgagagcag tgcctgaggt agcgcttcgc gccgctcggc ctcgttgaga tcggccaccg
     3061 gccatcccag cagctcggcc gcgcgcaggt ggcgctcggg ggcggtggtg taggtgaagc
     3121 ggaaggcggc gggcgcagtg aggatgaccg acatgccgtg cgggacgagc gggtgccggg
     3181 tgcggtagcc gctcgggatg tagtcgcgca ccaggcccgc gatcgggtac gccaccgcgt
     3241 gggggatgtg cacccccgcg ttgccgaagc cgatgcccgc gtagttggcg gcgagggcca
     3301 ggtgcaggcg cgcctcgacg tccaggccgt tgagcacggc gcgccgcagg tagcgggccg
     3361 cgtactccat cgctttctcg caccagatgt cgctggccgg gttcgcgccg atataggccg
     3421 gccggtcggg cgggtgatgc ttggcgcgcg cgttgtacgg gcgcgtggtg tacgactcga
     3481 tggcgtgggt gaggatgtcg gcccccgccg ccgcggtcac ctcgggcggc gcggtgagcg
     3541 tgttgagcgg atccaccacc cccagcgcgg gccgcagcag gcggtgcgag acgcccgcct
     3601 tcacgttctg atccacgatg tgcgtgaccg ccaccgcggt ggtttcgctg ccggtgccgg
     3661 cggtggtggg caccgcgatc agtgggcgga ccgggccggg taccggcacg cccctcccga
     3721 ccggcttgtt gatgtagtcg agcagcggcg ccgggtagca ggtgaggagg ttggcggcct
     3781 tgcacgtatc gatggtgctg ccgccgccca ccgcgatcag cccgtcgaag tccttgctgc
     3841 gcgcgtactc gcccgcctcc tccatcgacc gatcggtggg ctcgacctcc acgccgtccc
     3901 agacgtcggc cttcacaccc tgctcctcga ggagtgcccg cacgcgctcg gagaggccca
     3961 gctccgagat gtggcggtcg gtgaagatca gcgccttgcg gatgcccagg cggctggcgt
     4021 cgaagccgat ctcgtccatg gcgcccagcc cgaacttgat cggcgagttc tccatcacga
     4081 acacggtttc ggtcggcagg gtcacgccct tcatgagagc ctccagtggg tggcggtgcg
     4141 tggaatgtcc ggctcgtccg agattataga atcccgcggc cgactgcgat accctcgggc
     4201 attccggttc ctgggttgcc cccttgatag aggaggaaga ccgatgccgc tcttcgcctg
     4261 gatcgcggcc ctgttgctga cgattggcgc ctcgccggcg cacgcccagc cggggcagat
     4321 cacgatcgtt cagcccgcgg aggccaccac catggatccg gggcggagca cccaggtcct
     4381 gaccgtcaat tacttctaca atctttacga ctcgctcctc cggtgggaca ccgcgctgca
     4441 gctccggccc ggcctggcca ccgcgtggcg ggcggtgaac gacaccacct gggagttcac
     4501 gctgcggtcg ggcgtcaaat tccacgacgg gtccccgctg accgcggacg acgtgcgggc
     4561 cacgctcgag cggaacctga tcccgggcaa gacggtggtg accgccggtt tcaccaccat
     4621 cgagtcggtg caggcggtca cgccgaccct cgtgcgcgtg atcaccaaga aggcggatcc
     4681 gctgctgccg gtgcgcatgg cccagatggg ctcgcaaatc ctgcccgccc ggctcaccac
     4741 tgacgacggg gtgaaggagc tggcccgccg gccggtcggc agcggcgcct accggttcgt
     4801 ggagtgggta aaggacgagc ggctcgtgat ggaagccaat cgcgactggt gggggtggga
     4861 gggccgcgct cccgcgatcg accgcgtggt ctggaaggcg atcccggacg acttcccccg
     4921 gctggtggcc ctcgagaagg gcgacgtcga catcgtcacc agcctgcctc ccgaccggat
     4981 caagtccatt gcggagggcc ggaacacgcg ggtggtgagc gtgccgtcct cacgtaccgt
     5041 ggagttcaac atcaacacca ctcagccgcc gctgtccgac aagcgggtgc gccaggccat
     5101 gcactacgcg ctcgatgtgc ccgcggtgat ccgcagcctc ttcgcggggc tcggcaagcc
     5161 catggccggc gcgctcgccg acaccgactt cggctacaac gcggccatca agccctatcc
     5221 ctatgacccc gggcgggcgc gggccctgct cggagaggcg gggttcgcgg gcgggatcga
     5281 cgtgacgctg cacgcgggca tgggcacgat ggtcaatgac aagcagctcc tcgaggtggt
     5341 ggccgacatg tggtcgaagg tcggcatccg cgccaaggtg gccatgatgg agatgggcgc
     5401 gcgccaacgc atggtcaacg agcgggccct gcctccgggc ggcctgcttc tgatcaacgt
     5461 gcagtccacg ctgctggatg ccgacggcag cctgtggcgc atcatgcatc cgaccgggct
     5521 gggcgggaag tactgggccg gcagccagcc cggccaccgc ttccacgagg tgatggagca
     5581 ggcccgctac gcgctcgatc cgaagacacg caaggctctc tacgtcgagg ctacccagat
     5641 catcgacgac gagaagccct ggctcgagct cttccaggag gtcatcgtgt acgggacgtc
     5701 caagcgcgtc gcgttccgcc cgcgcccgga cttccgcctc atcgtgtcgg agatgtcgct
     5761 cagccgctga acgcggccgc gccgagcctt caccgacacg gggtgcccac gacggtgggc
     5821 aagggccttc ggaggagatc gagctaggaa ggaagcgcga cgcgagcgag aagcttgacg
     5881 tcgtccagca gcgctccggc gccgcggacg acgcaggtca gcgggtcgtc gctcacgcgg
     5941 atgggcaggc cggtctgctc gtgcaggagc agatcgagtc cgcgcagcag cgcgccaccg
     6001 cccgacacca cgatgccctt gtccacgatg tccgcggcca gctcgggcgg aatctgctcg
     6061 aggcacgtgt gcaccgcgct cacgatggcc atgatcggct cgcgcagcgc ctcgcggatc
     6121 tcctcgccgg tcaccaggat ggtcttgggg atgccgacga tcaggtcgcg gcccttgacc
     6181 tccatggtga ggtgctcgcc cgcgaccggg taggcggagc ccaggcacag cttgatgtcc
     6241 tcggcgtggc gctcgcccac gagcaggctg tagttcttgc ggatgtgctg gatcaccgcg
     6301 ttgttcatct cgtcccccgc gatgcgggcg gacttgcagt agacgatgcc ggacagggac
     6361 atcaccgcga cctcggtggt gccgccgccg atgtccacga tcatgttcgc gcccggctcg
     6421 gccaccggga gacccgcgcc gagggctgcc gcgatgggct cctcgatcag ataggcctca
     6481 cgggcgcccg cctgcttggc cgactgacgc accgcccgct tctccacctg ggtgatcccc
     6541 gagggcaccg tgaccacgac ccgcgggtgc acgatgctgc gacgctggtg ggtcttgcgg
     6601 atgaagtagt ggagcatctt ctcggtggag tcgaagtcgg cgatgacacc gtggcggagg
     6661 ggccggatga ccgagatgct cccgggggcg cggccgagca tggccttggc ttcgtggccg
     6721 gcggccagca cgacgccgtc cttcatggcg acgatggacg gctcgttgag gacgatgccc
     6781 tgtcctctca cgtacacgag agtgttggcg gtgcccagat ccaccgccag gtcatcggcc
     6841 aggaagctca tgagggatct cagacgcatg tacggtcctt tcgggacggg gaaggaacgc
     6901 aacgggtatg ccaggggcat gggcaggagc ggggacgcga agtggttgcc gaaggcccgt
     6961 ctcctaccat gggcgggggt gcacaggcgc cggtcttcgg cgaaagtgtc agcataggta
     7021 tgttttacat tacaccaatg taagatttac cccggtttct gcccggaaag ggccttgtca
     7081 gcccgggggc cccgtcgtaa tcttgggcgc catgaaggcc ccccggaagc ccccggccaa
     7141 gatcgaaggc cgcaagcgca tgcagcagag cggtctcacg gtcgcctggc acggcacgcg
     7201 gagccgcaag gattgggcgg cgtatgtcga gaacgggagc gagcaggggc gcctcgtgct
     7261 cgccgagcag gtgtccgagc gggccgtgaa gagcctgctc gcgcggatcc agagcctctc
     7321 gcgggatgca ctcgaggcca tgggtcgccc ggccgaggcg gtcgccgcgc agggcggagg
     7381 gccgccggtc cggcggccgc gcgggggcgg ggaggccagc tcgagctccg agatcatcgt
     7441 gcagccgctc cccggccgcc agctcgccct gagcggcgac gcggaggcgc ccgccaccac
     7501 gagcccgcgt cgccgccggc ttcccgccaa ggtggagcgc aatgcccctg ccgagcgccg
     7561 ccccgcgcgg ctctccgccc gagatctggc ggggacggtg cggcaactgc gctcccggct
     7621 ggagagcctg gaagaggagc aggaccacct acgggccgag ctcgccctgc tgcgcggcga
     7681 cgccgaggcc tacgagggga cgccgagcat cttcgtcacg gggtggtttc gcgcgaccct
     7741 ggtcctgatc gtgctggcga ttctggtggt gatcacggtg ccgtggctga tggacatctt
     7801 cgaagggggg gcgcgggagt cgcgcccccc gatccgcaac gaagcgccgg cggcctcgcc
     7861 gaacccgacc ccgagcggtc gctgagccta ctgttccgcg cagcgcaacg cgacgtgggc
     7921 gtactcgatg ccggcggggt ggtagtggat cgtccccatg ttgccctcgc gctcgaaggt
     7981 gtgccccgac cagctatcgt tgatcgcctt gaccgtgacc agatgtcgct ccagcgcggc
     8041 cttgcactcg tcgccgctgg cgtagcggcc ggcgatgatg cggtcggtct gcagccgcgg
     8101 gacgcgggcc agccagcgca ggacgatggt gtcgagcggg ttgtccttga cgatcacgga
     8161 caagtcggac cagagaagcc agaccagact cagggccccg atccactttc tcatgggcag
     8221 actctaggtc cgggcccgcc gactgtcaag gttggggccc acgggaaccg ccgggtcgcc
     8281 tagtgcgttt gtgcacaagc gtgattcgcc gacgcgagcg cgaaaaaccc gggcgaggga
     8341 ggtagaatag ggccgcagat ggagaccatc ctggtggtgg acgacgaagc gcaggtgcgg
     8401 accctggcgc gtgacatcct gttgggagcc ggctatcggg tgctcgaggc cgaggacggc
     8461 gagcaggcgc tgcgggtcgc ggaagatcac ccgggtgcca tccacgtgct cctcaccgac
     8521 atcatgatgc cgggtatcaa cggcaaggag ctggcggatc ggttcctcgc cgcgcgcccg
     8581 gacgccaagg cgatcttcat gtcgggccgg gccgccgagg tgatcagcga cgccggcgtg
     8641 ctcattccgg tggacgcctt cctcgccaag ccattcacgg tggagcggct gctgaacaag
     8701 gtccgcgagc ggctcgagta tcgctcgccc ttctcccggc cgcgctagtc gtctgaccag
     8761 cgccgacgac ccgatcgagc tcctctttgc ccggggggtg accgacggcc tcccggcggt
     8821 gcctcccacc gcggcgcgcg tcgcggcggc ggtggccgcc tcgggtcgat cccgcgacga
     8881 gctggtggcg gagatgccgc cgaacttcgg gcgcgccacc gtcgagaaga tcgcggtcaa
     8941 cgcggtgatg acgggctgcc ggcccgagta cttcccggtg gtgctggccg cggtcgaggc
     9001 gatgtgcgac gaccggttct gcctgctcgg cgtctccggg atcaccgacg cggtggcccc
     9061 gctcgtgatc gtgaacggcc cgatccggca gagcctcggc atcaactcgg gcgcgggcgt
     9121 cttcgggccg ggctggcgcg ccaacgcgac gatcggccgc gcggtgcggc tctgctgggt
     9181 caacatcggc ggtgcgcaac ccggcgtcat cagcatgtcc acgttcgggg gccccgggcg
     9241 ctacacgtac tgcatcggcg agaacgagga ggcgagcccg tgggagccgc tgcacgtgga
     9301 gtacggcttc gctcgcgagg agagcacggt ggcggtgctg gcggcggagg cgccccagat
     9361 cgtggccgac acacgaagcc ggaacgcccc ggatctgctc gccacgattg cccggagcgt
     9421 cgaggtggtc gcgcatcaca aggccacggg gctcggcgac accgcgcttg tcttctcgcc
     9481 cgagcacgtg accacgctgg ctgcggacgg ctggtccaag gcccgcgccc ggcagttcct
     9541 ctgggagcgc ctccgcaagc cggtgcgcga gctactgccc ggccgggacg gcggagaagg
     9601 gctctccgac gaggcgctgg ccgcgttccc cgcggagggg cgcagcgacg cgctcgtgcc
     9661 gaagtttcag gcgccggaga gcctcaagct cctggtggcg ggcgggcccg cgggccgttt
     9721 cactgcgatc gtacccggct ggccgttccg caacgcaccc tcgcggctgg tgttccggaa
     9781 gatcgcgccg agctaaccgc tgtccatctg ggcggggcgg ttcgcccggt cgccgggtcg
     9841 gagattcaaa atggcctccg ggaacacgcg cgcgagccat cccaccacgt cgttcacggt
     9901 cacgacaccg atcgggcggt gatcgccgtc caccaggggc acggtgcggt agcccgcact
     9961 gtgcatgcgg ttgatggcga agcagatgcg gtcgtcgggg gtgagcgcct cgggatcggc
    10021 ggtcatcacc tccgccaggc gcgtccggtc gagctcgcgc cccaggcgga ccacccgcgt
    10081 gagcaggtcg cgctcggtga agatcccgac gagccgtccg gccgggtcca cgatcaccac
    10141 gccggcccgg tggttgtcga ccatccgcgt gaccgcctcc gcaacggagg catccggggg
    10201 caggcggatc ggctcgctgg gggcgagcag ccgtacctcg tcgctgagca gggcgtcttc
    10261 gagcttgcgg aactccgcgt cgagggactc ggagtactcg tcgtggaact cgctcatggc
    10321 cgccacctca aggggtgcag catgctaggc gcgcggcgaa ggccgtgtca agagtgggaa
    10381 ggcgcatgcc tagtcaggga gcgggggcgc ccaagcttgg cgggaggctt cccatcaccc
    10441 tgcgcgaggc gctgcgtgcc tggaaggcgg catcgagcgg tcgccgatgc cgcttgaagc
    10501 cccgtgtcgg gcgaaggggg ttaagtgctt gatgacgctg ctttcactgt atgcaagtga
    10561 tgcagggtct gcctgcgggt gcggagacga cgctcccgtt caggaagctg cggccgccgc
    10621 agcgggatgg caccgagttt gccgagtaag gaaagccttg aaagtcattt gcccgcggtg
    10681 tcagcaggaa ggcaagccca gcctcctggt cacgatcgac atcccgggcg acgaccgtga
    10741 gatccacggg ttgtgctgga cgcaccggct tcgcctgctg gccaagtcca ccgacgtacc
    10801 gccctctcgc gagcccggga agcgttgatg atgctgctgg gcggacgact ccgccggatg
    10861 tcgatctggc tctggcgtac cactcccgcc cgccggctgc tcgtgatcgt cggccccggc
    10921 caccccacgc tcgtgccccg gatcgagcga ctcttcgagg gcgaacccgg cgccgtggac
    10981 gtggtgctcg accggaggca gcgggagcgc cgccgcgtgg tcatgacgcg ccgacccgag
    11041 cggcggcgcc gggaccgacg ccggcagcac ggcgcggccc ggcggaccgg cgacgtctcg
    11101 gtggtgattc ggcgggaatc ggcgcccgga ctggattaca ctccccccat tcagacgaaa
    11161 ggagaaggtc gaatgatgag tgtcgagtcc aagcaaacgc gtgacggaga cgacgtgcgg
    11221 cgctggctcg aggccgggca gacccagctc gcctcgctcc tggagctgct gcacgagcac
    11281 gaccgcctcc gcgagcgcgt ggattcttcc gagcgggaga acgagcggct ccgcggcgtg
    11341 acgtacgaga acgagcagct ccgcaaccgg ctcgagacct ccgagcgtca ggcggagcac
    11401 ctccgccaga gcatgggcga gctgcgcgcc gagaacgagc ggcaccagaa ggagcgcgag
    11461 gaggcggccg agcggctcaa ccatctcgtc aacgagatcg cgcaacgcct gcgccccggc
    11521 gcgggagtct cgcggacctg agaaccgcgt cgccaatttt tgtccgcttc ccggtggccg
    11581 cgagtcgccg gacggcggag cgcgcggttt tcagggggtt tgcgggccgc ccccgttggc
    11641 actcccgatg cagccagatg agtgtcgatc gcgacgtgca gaaacttccc tatgaccgat
    11701 acggaaaacg cgaggccgcg ggtcctgctg gtggacgacg aggaaggcgt gcgcatgagc
    11761 ctgcgcgccc tgctcgagcg cgagtaccag atcgcgagcg tgccgagcgg cgaggcggcg
    11821 ctggaggagc tgccgcgctt ccgcccagac ctggtactgc tcgacgtacg cctgctgcct
    11881 agcatgagcg ggctcgacgc actcgagcgc atcaaggcgt tcgacgagcg catcgaggtc
    11941 gtgatgatca ccgccttcgc ttcgctcgcg accctcaacg aggcgctgcg ccgcggtgcc
    12001 tgcggctatc tcatcaagcc gttcccccgc cgggatctgg agctgatggt tcgcgacgcg
    12061 ctggcgcgcc gcggccttcg gctcaactga ttggctcgaa cgcgccgttg acaggattgc
    12121 ctcccgcggt cagaatttgt ccagacgctc catcccaagc gacgggaggg tatcgcccat
    12181 gagcctcacg agtcaggtcg agaagtggg
//
LOCUS       DASUFA010000314         5884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1206973, whole genome shotgun sequence.
ACCESSION   DASUFA010000314 DASUFA010000000
VERSION     DASUFA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206973"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VFX87_11730"
     CDS             complement(<1..1305)
                     /locus_tag="VFX87_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4995638.1"
                     /translation="MPRVRLLLVLALCLMPASGASAQATAPAVSPLAVTEATLDNGLR
                     VLVQDDPRNPIVAVQIFYRVGSRNERPGATGLAHFLEHMMFKGTPTYGRGQIARLIEE
                     NGGRDNAYTSKDLTSYYTNIAADKLDLVLRIEADRMRHLLLDAAEIDSERKVVTEERR
                     MRSEDDPDGLVYEAMSSLAFKAHPYRWPIIGWMSDIARINQAELRAFYNTYYLPNNAI
                     LVIAGDVRAPAALAMVRRHFAGLRRGPAPPPVTAVEPPAIDERRLVVRREQAQLPVVN
                     IAWHVPNHTSPDAPALEMLSTLLSSGRASRLYQKLVYEKRMVLGAGGDYSYFSLDPSL
                     FWFYAAPLPGQAPEAVEQALLAEIERLKQEVVPEEELARARNQIEASFVWQQDSVFSR
                     ASVLGRFEMLGSWRLLDAYLPKLRAVTTADLQRVARTYFALDR"
     gene            complement(1322..2044)
                     /locus_tag="VFX87_11735"
     CDS             complement(1322..2044)
                     /locus_tag="VFX87_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX4995639.1"
                     /translation="MVGQSGAQDKSRRDGLAALARRIVRCRLCPRLVRHREAVAAAPP
                     RRYRGQRYWARPLPGFGDPDARLLLVGLAPAAHGGNRTGRMFTGDRSGDWLFRALHEA
                     GFANQAASTDPGDGLRLQGAYITATVRCAPPANKPTPPEMDRCRPFLLEELRLLSRVR
                     VVVALGKIGWDAYLRSRRAMGLAVPRPLPRFGHGAVVAMPDGGPRLIGCFHPSQQNTF
                     TGKLTREMLRAVFGRARELAGF"
     gene            2077..2940
                     /locus_tag="VFX87_11740"
     CDS             2077..2940
                     /locus_tag="VFX87_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX4995640.1"
                     /translation="MTSRVPALIVHGGAGADPGGREELRGGMRDAVAAGWRALGAGGT
                     ALDAVEAAVRSLEDHPRFNAGRGSVLTAGGTVETDASIMEGDQLRNGAVAAVSGVRNP
                     ITLARRILEAGRHSLFAGPGALARAEALGVPLCDPAELVTEPQRRRLAALQAGTVGAV
                     ALDRFGTIAAATSTGGTSGKLPGRVGDSALIGCGTYAESTLGGVSCTGDGEAIIRVTL
                     ARRALDILKTVGEPGHACEVALSVLVEEGRGQGGLIVVDWRGRVGWARSTALMPVGFM
                     SPAHAEPELPF"
     gene            2951..4165
                     /locus_tag="VFX87_11745"
     CDS             2951..4165
                     /locus_tag="VFX87_11745"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX4995641.1"
                     /translation="MIAAADIRAARARLAGIVAATPCPHSETLSAMTGARVFVKLENL
                     QMTGSFKERGAANLLLQLSPDERRRGVVAASAGNHGLGVAFHAAKLDVSAVITMPEWA
                     PLAKVMAARRYGAEVILHGENFEEAHARAREIEAERGLVFVHPFDDPRVIAGQGTIGL
                     ELLAQVPDLDAVLVPVGGGGLIGGVATAIKAERPAVKVIGVQAREIAAMQAALAGDAR
                     DLLPAATTIADGIAVRRVGEQTLALARRHVDAMVTVSEEEIANAILLLLEIEKTVVEG
                     AGAVPLAALANRRVALEGRTVALVVSGGNIDVNLIARVIERGLVKDGRLVRLRVRLRD
                     RPGALAALTAVVAHTRANVLGIEHDRAFSRAPLGDSEVELTLETSGPEQIEAIKRRLT
                     EEGYVAEEVSPA"
     gene            complement(4175..5254)
                     /locus_tag="VFX87_11750"
     CDS             complement(4175..5254)
                     /locus_tag="VFX87_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4995642.1"
                     /translation="MSPPDGLRGWLQRLGWSRPPERRALVLAGGGVIGGMYEVGALAA
                     LDEALPGFRPNDFDLYVGSSAGSVVAALMANGVRPRELYAILDEERDDPLNFNRGAVY
                     HKGSFVGATRNLAQFMWAVGKRAVVDFRLEWPDLLARSGGDMPAGFFSLAPLEAYLRE
                     AFQAKGLSNSFSGTPRPLLVAAMALDRAERVVFGQGDLMDVPISQAIAASSAIPGFFE
                     PYRIRDRDYVDGDVGYTGHTDLAVDAGATVLVAINPAVPQRLDEMAGPEIRRGGLYAI
                     MEQAGHISSLNLFNLGLREVKLLHPEMEILVIQPEPKPSPLVGPSMGFEASRAALRYG
                     YSTVKDWLAGPGGAAAARFAEHPTR"
     gene            complement(5251..>5884)
                     /locus_tag="VFX87_11755"
     CDS             complement(5251..>5884)
                     /locus_tag="VFX87_11755"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX4995643.1"
                     /translation="QLIRGGMYRRVLVVGAELLSSAFDYTDEGRDMAVLFADGAGAAV
                     LEAVETDAAHPRGLISHHLHSDGTALDALSGELWGSSTFPPVSKKKIDDGRTRPRMNG
                     RAVFVNAVRRIREVVQECLDANGLRAADVSCYLFHQANLRIIEAAAEHFQIPAERYFI
                     NLDRYGNTAAASVPICLHEALAEGRIKNGDLVMLVAFGTGFSWGATLLRW"
BASE COUNT          827 a         2071 c         2197 g          789 t
ORIGIN      
        1 gcggtcgagg gcgaagtagg tccgggccac ccgctgcagg tcggcggtgg tgacggcgcg
       61 gagcttcggc agataggcgt cgagcaggcg ccaggagccg agcatctcga agcgccccag
      121 caccgaggcg cgggagaaga ccgaatcctg ctgccagacg aaggaggcct cgatctggtt
      181 gcgggcgcgg gccagctcct cctcgggcac cacttcctgc ttgagccgct cgatctcggc
      241 cagcagggcc tgctcgaccg cctcgggcgc ctggccgggc agcggcgccg cgtagaacca
      301 gaagagggac gggtccagcg agaagtacga gtagtcgccg cccgcgccca ggaccatgcg
      361 cttctcgtag accagcttct ggtagagccg cgaggcgcgg ccggatgaca gcagggtgga
      421 cagcatctcg agcgcggggg catcgggcga ggtgtggttc ggcacgtgcc acgcgatgtt
      481 gacgaccggg agctgcgcct gctcgcggcg cacgaccagc cggcgctcgt cgatcgcggg
      541 gggttcgacc gcggtcaccg gcggcggcgc gggcccccgg cgcagccccg cgaagtggcg
      601 ccgcaccatc gcgagcgcgg cgggcgcgcg cacgtccccc gcgatgacca ggatcgcatt
      661 gttgggcagg tagtacgtgt tgtagaaggc gcgcagctcg gcctggttga tgcgggcgat
      721 gtccgacatc cacccgatga tgggccagcg atacgggtgc gccttgaacg cgagcgagct
      781 catcgcctcg tagacgagcc cgtccggatc gtcctccgag cgcatccgcc gctcttcggt
      841 cacgaccttc cgctccgagt cgatctcggc ggcgtccagc agcaggtgcc gcatccggtc
      901 cgcctcgatc cggagcacca ggtcgagctt gtccgccgcg atgttcgtgt agtagctggt
      961 caggtccttg ctggtgtagg cgttgtcgcg gccgccgttc tcctcgatga ggcgcgcgat
     1021 ctgcccgcgg ccgtaggtgg gcgtgccctt gaacatcatg tgctcgagga agtgggcgag
     1081 cccggtcgcc ccgggccgct cgttgcgcga gcccacgcgg tagaagatct gcaccgcgac
     1141 gatggggttg cgcgggtcgt cctgcacgag cacccgcagc ccattgtcga gcgtggcctc
     1201 ggtgaccgcg agcggcgaga cggccggtgc ggtggcctgc gccgacgccc ccgacgcggg
     1261 catcaggcac agggcaagaa ccaggagcag acgcacgcga ggcatgcgtc catggtaaca
     1321 cctagaaacc cgcaagctcg cgcgcgcgac cgaacaccgc ccgcagcatc tcccgcgtca
     1381 gctttccggt gaaggtgttc tgctggctcg ggtgaaagca gccgatgagc cggggcccgc
     1441 cgtccggcat tgccacgacc gcgccgtgac cgaagcgcgg cagcggccgc ggtaccgcga
     1501 gccccatcgc ccggcgcgag cggaggtagg cgtcccagcc gatcttgccg agggcgacga
     1561 cgacccggac gcgggacagc agccgcagct cctccaagag gaatgggcgg cagcggtcca
     1621 tctccggcgg ggtcggcttg ttggcgggcg gcgcgcagcg cacggtggcc gtgatgtacg
     1681 cgccctggag ccggaggccg tcgccgggat cggtcgaggc ggcctggttg gcgaagccgg
     1741 cctcgtggag cgcgcggaag agccagtcgc cgctccggtc gccggtgaac atgcgcccgg
     1801 tgcgattgcc tccgtgcgcc gccggcgcca gtcctaccag cagtagccgg gcgtccggat
     1861 ccccgaagcc cggcagcggg cgcgcccagt agcgctggcc gcggtaccgg cgcgggggcg
     1921 ccgccgcgac tgcctcgcga tggcgcacca ggcgcgggca gaggcggcag cggacgatgc
     1981 gtcgcgcgag ggcggcgagc ccatcgcgtc gcgacttgtc ctgtgctccg gattggccga
     2041 caatcaccgg ggcatgatag cgatcggcgc cgagccatga caagccgcgt ccccgcgctg
     2101 atcgtgcacg gcggcgccgg cgccgatccg ggcggacgcg aggagctgcg cggcggcatg
     2161 cgcgacgcgg tcgccgcggg ctggcgcgcg ctcggcgctg gcggcaccgc gctcgacgcg
     2221 gtggaggcgg cggtgcggag cctcgaggac catccacggt tcaatgccgg gcggggctcc
     2281 gtcctcacgg cgggcggtac cgtggagacc gacgcctcca tcatggaggg cgaccagctc
     2341 cggaacggag cggtggccgc cgtctcgggt gtgcggaatc cgatcaccct ggctcggcgc
     2401 atcctcgagg ccggccggca ctcgctcttc gcagggcccg gcgctctcgc ccgcgccgag
     2461 gcgctcggcg ttccactctg cgaccccgcc gagctcgtga ccgagccgca gcggcgccgg
     2521 ctggcggcgc tccaggcagg cacggtcggc gcggtggcgc tcgatcgctt cggcaccatc
     2581 gcggcggcca cctccaccgg cggcacgtcc gggaagctgc ccggccgggt gggcgacagc
     2641 gcgctcatcg gctgcggcac ctatgccgag agcacgctcg gcggtgtgtc gtgcaccggc
     2701 gacggggagg ccatcatccg cgtcaccctg gcccgccgcg cgctcgacat cctgaagacc
     2761 gtgggcgagc ccggccacgc ctgcgaggtc gcgctgagcg tgctggtgga ggagggccgc
     2821 ggtcagggcg gcctcatcgt cgtggactgg cgcggtcggg tgggctgggc ccggtccacc
     2881 gcactgatgc cggtcgggtt catgtctccg gcgcacgccg agcccgagct gccgttctga
     2941 tcgcgcgcgc atgatcgccg ccgccgatat ccgagcggcc cgggcgcggc tggccggcat
     3001 cgtcgccgcg acgccctgcc cccactcgga gaccctctcc gcgatgaccg gcgcgcgcgt
     3061 cttcgtcaag ctggagaacc tccagatgac gggctccttc aaggagcgcg gcgcggccaa
     3121 cctgctcctg cagctctccc ctgacgagcg ccgtcgcggg gtcgtcgcgg ccagcgcggg
     3181 caaccacggc ctgggcgtcg ccttccacgc ggccaagctc gatgtctccg ccgtcatcac
     3241 catgcccgag tgggcgccgc tggccaaggt gatggccgcc cgccgctacg gggccgaggt
     3301 catcctgcac ggggagaact tcgaggaggc ccatgcccgc gcccgcgaga tcgaggccga
     3361 gcgcgggctc gtcttcgtcc accccttcga cgacccgcgc gtcatcgccg gccagggcac
     3421 catcggcctc gagctgctcg cgcaggtgcc ggacctggac gcggtgctgg tgcccgtggg
     3481 tggcggcggc ctcatcggcg gcgtcgccac ggccatcaag gcggagcgcc cggccgtgaa
     3541 ggtgatcggg gtgcaggcgc gcgagatcgc ggccatgcag gcggccctgg ccggcgacgc
     3601 gcgtgatctc ctgcccgccg ccaccaccat cgccgacggg atcgcggtcc gccgggtcgg
     3661 cgaacagacg ctggcgctgg cccggcgcca cgtggacgcc atggtgaccg tcagcgagga
     3721 ggagatcgcc aacgccatcc ttctgttgct ggagatcgag aagacggtcg tggagggcgc
     3781 cggggcggtc ccactggccg ctctggccaa ccgccgggtg gcgctcgagg gccggacggt
     3841 ggccctggtg gtctcgggcg gcaacatcga cgtgaacctg atcgcccgcg tgatcgagcg
     3901 cggcctggtc aaggacgggc ggctggtgcg cctgcgcgtg cggctccgcg accggccggg
     3961 agccctggcc gcgctgaccg ccgtcgtggc ccacacgcgg gccaacgtgc tcggcatcga
     4021 gcacgaccgg gccttctcgc gcgcgccgct cggcgacagc gaagtggagc tgacgctcga
     4081 gacctccggc cccgagcaga tcgaggccat caagcgccgg ctgaccgagg agggttacgt
     4141 ggccgaggag gtcagcccgg cctgacggcg gttttcagcg ggtcggatgc tcggcgaacc
     4201 gggcggcggc ggcgcccccg gggcccgcga gccagtcctt gaccgtgctg tacccgtagc
     4261 gaagcgccgc gcggctggct tcgaaaccca tggagggccc caccagcggg gagggtttgg
     4321 gctcgggctg gatcaccagg atctccatct cgggatgcag aagcttcacc tcgcggagcc
     4381 cgagattgaa caggttgagg ctcgagatgt ggccggcctg ctccatgatc gcgtagaggc
     4441 cgcctcgccg gatctcgggt ccggccatct cgtccaggcg ctgcggcacc gccgggttga
     4501 tcgcgaccag cacggtcgcc cccgcgtcca ccgccaggtc ggtgtggccc gtgtagccca
     4561 cgtccccgtc cacgtagtcg cggtcccgga tccgataggg ctcgaagaac cccgggatcg
     4621 ccgaggaggc ggcgatggcc tgcgagatcg ggacgtccat gagatccccc tggccgaaca
     4681 cgacgcgctc ggcgcggtcg agggccatcg cggcgacgag tagcggccgg ggcgtgccgc
     4741 tgaagctgtt cgagagtccc ttggcctgaa aggcttcccg gagataggcc tcgagcggcg
     4801 ccagcgagaa gaagccggcc ggcatgtcgc cgccgctgcg cgcgagcaga tccggccact
     4861 cgagccggaa gtccaccacg gcgcgcttcc cgacggccca catgaactgc gccaggttgc
     4921 gcgtcgctcc cacgaacgag cccttgtgat agaccgcgcc tcggttgaag ttgagcgggt
     4981 cgtcgcgctc ctcgtccagg atcgcgtaca gctcgcgcgg gcgcaccccg ttggccatga
     5041 gcgcggcgac caccgagcca gcgctcgaac ccacgtagag gtcgaagtcg ttggggcgga
     5101 agccgggtag cgcctcgtcc agcgcggcga gggcgccgac ctcgtacatg ccgccgatga
     5161 ccccgccgcc ggccaggacc agcgcccggc gctcgggtgg ccggctccag cccagacgct
     5221 ggagccagcc gcggagaccg tcgggagggc tcaccagcgc aggagcgtgg ccccccacga
     5281 gaagccggtg ccgaaggcca ccagcatgac gaggtcgccg ttcttgatgc ggccctcggc
     5341 gagtgcctcg tggaggcaga tgggaacgga cgcggccgcc gtgttgccgt agcggtccag
     5401 gttgatgaag taccgctcgg ccgggatctg gaagtgctcg gcggccgcct cgatgatgcg
     5461 caggttggcc tggtggaaga ggtagcagct cacgtcggcc gcccgcaggc cgttggcgtc
     5521 gagacactcc tgcacgactt cccgtatccg ccggaccgcg ttcacgaaga cggcgcgccc
     5581 gttcatccgc gggcgcgtac ggccgtcgtc gatcttcttc ttggacaccg gcgggaacgt
     5641 ggacgagccc cacagctctc cggagagggc atcgagcgcg gtgccgtcgg aatggaggtg
     5701 atgcgagatc agcccgcgcg ggtgggccgc gtcggtctcc acggcctcga ggaccgcggc
     5761 ccccgccccg tcggcgaaga gcaccgccat gtcgcggccc tcgtcggtgt agtcgaaggc
     5821 ggacgagagc agctcggcgc ctaccacgag gacgcgccgg tacatcccgc cgcggatgag
     5881 ctgg
//
LOCUS       DASUFA010000315         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_752030, whole genome shotgun sequence.
ACCESSION   DASUFA010000315 DASUFA010000000
VERSION     DASUFA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752030"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1181
                     /locus_tag="VFX87_11760"
     CDS             255..1181
                     /locus_tag="VFX87_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX4995644.1"
                     /translation="MAQPPRTRTAYFNGRLVPETQVVIPYRDRSFNRGDGCFDMTRTF
                     DGRIFRLEEHVERLYRSLSYLGIDVGLEPKEMIAITEEVVERNEPLRAAAGDWWVAQR
                     ISRGVDAIGDEGWAHTGPQVIVDCTPLPLRKRAAHFRDGIDVVVPSIRRTPPSILSPR
                     AKTHNYLNLILADREVKRASPDAWSVLLDEHGNLCEGVGSNIFVVRGGRVYTPREQFV
                     LPGVSRRTAMEMAAQLGIPCEERDLDVYDAVTADEVFLTSTSLCLCGVRSFNGKTIGD
                     GRVPGPITKRLTDAYVALVGTDFVAQYLSRLG"
     gene            1187..2092
                     /locus_tag="VFX87_11765"
     CDS             1187..2092
                     /locus_tag="VFX87_11765"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4995645.1"
                     /translation="MTVRFDPTGPYEVDETDTPFARPAGAELLARIYRPRVAADRPLG
                     AVAYVHGGAWARLDRTADAIMCGALAASGLVVIALDFRQAPDHRFPVASADIAAGVRY
                     ARAHAGRLGVDAGRIGLLGSSSGAHLALLHAVQPGAPERAGTPIVRPDGSLDPTAGDA
                     SVAFAVALYPVADPLARYHYALSREHEPPPPSGFDAKRLVASHRAFFADEAAMAAASV
                     TRIVSGGRATALPPIWVAQPDQDDNVPAAITEAFVKAYQGAGGAVEHAHFPGARHGFV
                     QQASPDTDRCVALVRDFIGRQLGCP"
     gene            2092..2946
                     /locus_tag="VFX87_11770"
     CDS             2092..2946
                     /locus_tag="VFX87_11770"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX4995646.1"
                     /translation="MEHLFPIVRHPADHRRTPILVSVPHYGTQPLPDITRDDYSEPWF
                     ETFAYGFADTFVGDVYSDLHEHGATVLATPFSRMFVDVNRRRDDFEHHEGLVRSRRGV
                     VRTHTMRDVAIFARPLGLPALEARLCALYDPYYATLQDLLTDLRRAHGYALLLDGHTG
                     SPRRMKDHQVIIGTRHDATCAPQIVATVAEIFTRHGFEVHENVTGYTGGNIVATFGQP
                     WTRRIHALQLEINASLLMATSREEFIALISRGGIPEKAEANIARVRRCLEEVLAALPA
                     VLATVHEP"
     gene            2965..3708
                     /locus_tag="VFX87_11775"
     CDS             2965..3708
                     /locus_tag="VFX87_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX4995647.1"
                     /translation="MPNSDRKLEIVTEVPESAMVVFAHPDDAEIGSGGVIATWVAAGC
                     EVSYVLCTNGDAGTADRDLSPSELAHKRAHEQRAAADFMGVRHVEMLGYPDGRLEDTR
                     EFLGDVVRAIRRHRPHTVFVHDPYRMKGFQHRDHRKAGIATTDAVYPYARDHLHFAEH
                     ITKEGLEPHKVRELWYWGADEPDVIVDVTVGIERQIAALVRHQSQMPGFNVPEGVTIG
                     ERVKKGAAELGAGYGFEYGAAFRRLVARR"
     gene            complement(3657..4229)
                     /locus_tag="VFX87_11780"
     CDS             complement(3657..4229)
                     /locus_tag="VFX87_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX4995648.1"
                     /translation="MLGVLAGLALSAAVGFRVFVPLLLTGSAARLGYLDLTADMAWLA
                     SDPALVALATATVLEVAAYYVPWLDNLLDTVATPTAILAGVITWAAVAPELSPLLRWT
                     LAVVAGGGAAGLVQSGTTFLRLHSSAFTAGLGNPVVASGELVGSVGLSVLAVLAPVLA
                     GVLVVLLFAGLVGLYRRATSRRNAAPYSKP"
     gene            complement(4245..>4433)
                     /locus_tag="VFX87_11785"
     CDS             complement(4245..>4433)
                     /locus_tag="VFX87_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995649.1"
                     /translation="VIVGSRDEYLDRRAAELVAAFERNATGARAFTGIIVPRAPHGFR
                     GHEPVLAREVVRWARTVR"
BASE COUNT          668 a         1704 c         1486 g          575 t
ORIGIN      
        1 ctggcactgg ttcctgccga tgctggacga ggcgcagagc gccgtcgacg cgatcggccg
       61 gttcgtgagg agccgcgcgg cctgacggcc gccccgagcc gggcgccccc gccgcggcga
      121 cccgaccgcg gcgagagggc ccctgtccag gtcgcgacgc gaccagcacc ggccacgcgg
      181 agcatccagc ccctggccgc gagcgctgcg gacggtcgcc gatctggagt agtatcccgg
      241 tgggaggaag atcgatggcc cagccaccgc gcacccgcac cgcgtacttc aacggccggc
      301 tcgtccccga gacacaggtc gtgatcccct accgcgaccg cagcttcaac cgcggcgacg
      361 gctgcttcga catgacgcgc actttcgacg gccgcatctt ccgcctcgag gagcacgtcg
      421 agcgcctcta tcggtcgctc tcgtacctgg ggatcgacgt cggcctcgag cccaaggaga
      481 tgatcgccat caccgaggaa gtcgtcgagc ggaacgagcc cctgcgtgcc gccgccggcg
      541 actggtgggt ggcccagcgg atcagccggg gcgtggacgc catcggtgac gagggctggg
      601 cgcacaccgg gccccaggtg atcgtggact gtacgcccct gccgctccga aagcgagcgg
      661 cgcacttccg cgacggcatc gacgtggtgg taccctccat ccgccgcacg cccccgtcga
      721 tcctcagccc ccgggccaag acccacaact atctgaacct catcctggcc gaccgcgagg
      781 tcaagcgcgc cagccccgac gcctggtcgg tcctcctgga cgagcacggc aatctctgtg
      841 agggcgtcgg cagcaatatc ttcgtggtgc gcggcgggcg cgtttacacg ccacgggagc
      901 agttcgtgct ccccggggtg agtcgccgga ccgcgatgga gatggccgcg cagctcggca
      961 ttccctgcga ggagcgcgac ctcgacgtct acgacgcggt gaccgccgac gaggtcttcc
     1021 tcacctcgac cagcctgtgc ctctgcggcg tgcgctcatt caacggcaag acgatcggag
     1081 acggccgggt gcccgggccc atcacgaagc ggctcaccga cgcctacgtc gcgctcgtcg
     1141 gcaccgactt cgtggcccag tacctgtccc gcctcggctg atcgacgtga ccgttcgctt
     1201 cgatcccacc ggcccgtacg aggtggacga gaccgacacg cccttcgccc ggccggcggg
     1261 cgcggagctc ctggcccgga tctaccgccc ccgggtggcg gccgaccggc cgctcggcgc
     1321 ggtggcctac gtccacggcg gcgcgtgggc ccgcctcgac cgcacggccg acgccatcat
     1381 gtgcggggcg ctcgccgcgt ccgggctggt cgtgatcgcg ctcgatttcc gccaggcgcc
     1441 ggaccaccgc tttccggtcg cgagcgccga tatcgccgcc ggggtccgct acgcccgggc
     1501 ccacgcgggc cggctgggcg tcgatgcagg gcgcatcggg ctcctcggca gctcgagtgg
     1561 cgctcatctg gccctcctcc acgcggtgca gcccggcgcg cccgagcgcg cgggcacgcc
     1621 gatcgtgcgg cccgacggct cgctcgatcc caccgcgggc gatgcctccg tggccttcgc
     1681 ggttgcgctc tatcccgtcg cggatccact ggcccgctac cactacgccc tgtcccgcga
     1741 gcacgagccg ccgcccccct cgggcttcga cgccaagcgt ctggtcgcct cgcatcgcgc
     1801 gttcttcgcg gacgaggccg cgatggcggc cgcgagcgtc acgcggatcg tctccggcgg
     1861 ccgggccacc gcgctgccgc caatctgggt cgcccagccg gatcaggacg acaacgtgcc
     1921 cgccgccatc accgaggcct tcgtgaaggc gtaccagggc gcgggcggcg ccgtcgagca
     1981 cgcgcacttc ccgggcgcgc gccacggctt cgtccagcag gcgagcccgg acaccgaccg
     2041 gtgcgtcgcc ctcgtccgcg acttcatcgg ccggcagctc ggctgcccct gatggagcac
     2101 ctcttcccga tcgtcaggca cccggcggac caccggcgca cgccgatcct ggtgagcgtg
     2161 ccccactacg gcacgcagcc gctccccgac atcacccgcg acgactactc cgagccctgg
     2221 ttcgagacct tcgcctacgg cttcgccgac accttcgtgg gcgacgtcta cagcgacctg
     2281 cacgagcacg gggctaccgt gctggccacc ccgttctcgc gcatgttcgt ggacgtcaat
     2341 cgccgtcgcg acgacttcga gcaccacgag ggcctggtgc gctcgcgccg cggagtggtg
     2401 cgcacccaca ccatgcgcga cgtggccatc ttcgcgcgac cgctcgggct ccccgctctc
     2461 gaagcgcgcc tgtgcgcgct gtacgatccc tactacgcga cgctccagga cctcctgacc
     2521 gacctccggc gcgcgcacgg ctacgccctg ctcctcgacg gccacaccgg cagcccgcgg
     2581 cgcatgaagg accaccaggt gatcatcggc acgcggcacg acgccacctg cgccccgcag
     2641 atcgtggcca cggtggcgga gatcttcacg cgccacggct tcgaggtcca cgagaacgtc
     2701 accggctata ccggggggaa catcgtcgcc accttcggcc agccctggac ccggcgcatc
     2761 cacgcgctcc agctcgaaat caacgcgtcc ctgctcatgg cgaccagccg cgaggagttc
     2821 atcgccctga tctcgcgcgg gggcatcccg gagaaggcgg aggccaacat cgcgcgggtg
     2881 cgccgctgtc tcgaggaagt tctggccgcc ctccccgccg tcctcgcgac ggtccacgag
     2941 ccataacagg ataagatcac cttcatgccc aactccgacc gcaagctcga gatcgtcacc
     3001 gaggtccccg agtccgccat ggtggtcttc gcccatcccg acgacgccga gatcgggtcg
     3061 ggcggggtca tcgccacctg ggtggccgcc ggctgcgagg tcagctacgt gctgtgcacc
     3121 aacggggatg ccgggaccgc cgaccgcgac ctcagcccct ccgagctggc ccacaagcgc
     3181 gcccacgagc agcgggccgc cgccgacttc atgggcgtgc gccacgtgga gatgctcggc
     3241 taccccgacg gccggctgga ggacacgcgc gagttcctcg gcgacgtggt ccgcgccatc
     3301 cgccgtcacc ggccgcacac cgtcttcgtc cacgatccct accggatgaa gggcttccag
     3361 caccgcgacc accgcaaggc cggcatcgcc accaccgacg cggtctaccc ctacgcccgc
     3421 gaccacctgc acttcgccga gcacatcacg aaggaagggc tcgagccgca caaggtgcgc
     3481 gagctctggt actggggtgc cgacgagccc gacgtcatcg tggacgtcac cgtcgggatc
     3541 gagcggcaga tcgccgcgct ggtgcgccac cagagccaga tgccgggctt caacgtgccg
     3601 gagggcgtga ccatcggcga gcgcgtgaag aagggcgcgg ccgagctggg ggccggctac
     3661 ggcttcgagt acggcgccgc gttccgccgg ctggtcgcgc ggcggtagag accgaccagg
     3721 ccggcgaaca ggagcacgac gagcaccccg gccaggaccg gggcgagcac cgcgagcacg
     3781 ctgagcccaa ccgagcccac cagctcgccc gatgccacca ccgggttgcc gagtccggcc
     3841 gtgaaggccg aggagtgtag ccgcagaaac gtcgtgcccg actggaccag cccggcggct
     3901 cccccgcccg cgaccaccgc cagcgtccac cgcaggagcg gagagagctc cggagcgacc
     3961 gcggcccacg tgatcacgcc ggccaggatc gcggtcggcg tggcgaccgt atccagcaga
     4021 ttatcgagcc agggcacgta gtaggcggcg acctcgagga cggtggcggt ggccagggcc
     4081 acgagggccg ggtcggacgc gagccaggcc atgtccgccg tcagatcgag atagccgagt
     4141 cgcgcggccg agccggtgag gaggagcggg acgaagaccc ggaagcccac ggccgcgctc
     4201 agggccagac cggccaagac tcccagcacg gcatccaccc gacgttacct caccgtccgc
     4261 gcccagcgga cgacctcgcg cgcgaggacg ggctcgtggc cgcggaaccc gtgcggcgcg
     4321 cgggggacga tgataccggt gaacgcccgc gcacctgtcg cgttccgctc gaaggcggcg
     4381 accagctccg cggcccgccg gtcgagatac tcgtcccggc tgccgacgat cac
//
LOCUS       DASUFA010000316         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1207648, whole genome shotgun sequence.
ACCESSION   DASUFA010000316 DASUFA010000000
VERSION     DASUFA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207648"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..986)
                     /locus_tag="VFX87_11790"
     CDS             complement(264..986)
                     /locus_tag="VFX87_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4995650.1"
                     /translation="MSLAWLVGAVVIAAACFVQGLAGFGIGLVSLAFLPFLMSPQHAI
                     VLITLYAAVFIVVIFIPLRRDFTLHGMTELVAGTILATPVGVWLLAGLPSDLLKRLIG
                     LVLLLIVALEWFGLYPERLHGRGWGFGAGLAAGLLGGAIGTPGPPVILYAAAQDWSPR
                     TVKANIQAFLIVNQAVILLGYWWAGLLDREVWRLTWLFAAPAVVGLAAGMLLFNRLDR
                     ARFRRVVFAVLFVSGLVLLIRG"
     gene            complement(998..2542)
                     /locus_tag="VFX87_11795"
     CDS             complement(998..2542)
                     /locus_tag="VFX87_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4995651.1"
                     /translation="MAWDERMKDFHARRAKARAMGGPERLARRRDTGTLNARERVERL
                     LDAGSFLEVGTFNTSDVPGMEAETPADSKVGGLGRIDGRPVVVCSNDFTVLAATSSRV
                     ASHKEAELKRLAARRGMPIVYLAEAGGARMPDIMGSAGIASFGNSTYYGTRRRRVPMV
                     TAVLGQAYGLPTWNACLSDFVVMLEGASMAVSGPRVLELATGEKISAEELGGAKAHAE
                     ETGFADVVGKTEDECFAFVKRFLSYLPSHNRQAPPQAEVPAGSGAEMSTIADLVPEAR
                     NRAYDMTRVLRRLADGGEIFQVKERFGRSLITALARLDGHVVGFVANQPIQKGGACDA
                     DGCDKAISFLALCDSFNIPLVFLHDVPGFFIGRDAERKRVAGKIITWMEALGMVTVPR
                     VSIIVRKTYGQAYFNMGGGGYADLLVAWPTAEMSFMDPATGVNVVHGAELAKLPAEER
                     VARRQELLAQWELDTLPYGAAARHLIDEVIDPADTRDVVIRFLAQARTRSAIGRHRLG
                     GWPTKF"
     gene            2637..4217
                     /locus_tag="VFX87_11800"
     CDS             2637..4217
                     /locus_tag="VFX87_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HEX4995652.1"
                     /translation="MKHHKVYRTELTPVSFLVRSAYVFPDKTAVVHGERRYTYRQLGE
                     RVNRLASALRGAGMQKHDRVAFLCPNIPAMLEAHYGVPAAGGVLVAINTRLSSDEIGY
                     ILRHSGSTFLFVDAELEHLIKPLDLTGTRVVRIDDTGAAGDPYEGFLAGGSPDPVESW
                     LEDEEETISINYTSGTTGRPKGVMYSHRGVWVNAVGEVVETGMTFDTKYLWTLPMFHC
                     NGWCFTWAVTSVAGTHVCLRRVEPARIWELIDAEGITHYNGAPTVQIGVVNDPKAHKL
                     ARPVTVTVAGAPPSPTLLGKLKELGFKPVHVYGLTETYGPHTVCGWNADWDALPAEEQ
                     ARLAARQGQGYALFDLVRVVDGEMNDVPRDGDTLGEVIMVGNNVAKGYYEQPDATSEA
                     FRGGWFHSGDIGVWHPDGYVELRDRKKDIIISGGENISTIEVEQCVARHPAVMECAVV
                     AIPDEKWGERPKAFVTLKPGQRATEPEIIEFCKQHIAGFKAPAAVEFGELPKTSTGKI
                     QKFVLRDREWKGRAKRIN"
     gene            4220..>5073
                     /locus_tag="VFX87_11805"
     CDS             4220..>5073
                     /locus_tag="VFX87_11805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995653.1"
                     /translation="MRVGILDQSPVRSGGTPADAVHETIELARAADRWGFHRYWLAEH
                     HSTPGLAGASPEVLIAQVAAVTSHIRVGSGGVMLPHYSALKVAEQFRMLETLHPGRID
                     LGIGRAPGSDQRTARILRHGPGTLGIEHYPRQIQDLVAFLHDDLPAEHPWRGVHAMPA
                     GPSVPEVWLLGSSDESALLAAQLGQSFCFAHFINEQGGAEVTRAYRERFCASPDLAAP
                     RSSVAVFAVCAETEAQARRLARSRDLFIVRLYTGRPAPYPSVEEAESYPYNAQEVAIA
                     QHAARRSVV"
BASE COUNT          796 a         1780 c         1819 g          678 t
ORIGIN      
        1 tcatcttcta ttcggtcgtg gtcctgctgt tcggatacgg gtatgtgttg ctgacgcatg
       61 cggggcgtcg ggtcctggcc tggcgtggca gggcccttgt tgcttgagcg gaccgtctcc
      121 gccggcggcc gaggcggatg aacaggcccg cggtgaggtg ctcgcccttc ggtgcagcgc
      181 ggacgggccg gatacctggg cggcgcagga ctaggccgcg gcgcggcgga gtgtcgaccg
      241 aggaacgccg ctcgacctac acctcacccg cggatcagta gcaccaggcc cgagacgaag
      301 agcaccgcga acaccacgcg acggaagcgc gcgcggtcga gccggttgaa cagcagcatg
      361 cccgcggcca ggccgaccac tgcgggcgcc gcgaagagcc aggtgaggcg ccagacctca
      421 cggtccagca ggccggccca ccagtagccg agcaagatga cggcctggtt gacgatcagg
      481 aaggcctgga tgttggcctt caccgtccga ggactccagt cctgggccgc cgcgtagagg
      541 atcacgggtg ggccgggcgt gccgatcgcc ccgccgagca ggcccgccgc gaggccggcg
      601 ccgaatcccc agccgcggcc gtggagccgc tccggataga gcccgaacca ctcgagcgcc
      661 acgatcagca ggagcaccag gccgatgagg cgcttcagca ggtcggacgg cagcccggcc
      721 aggagccaga ccccgacggg cgtggccagg atggtgccgg ccaccagctc ggtcatgccg
      781 tgcagggtga agtcgcggcg caacgggatg aagatgacca cgatgaagac ggccgcgtag
      841 agcgtgatca ggacgatggc gtgctgcggc gacatcagga acggcaggaa ggcgagtgag
      901 acgaggccga tgccgaagcc ggccaggccc tgcacgaagc aggcggccgc gatgacgacc
      961 gcgccgacca gccaggcgag cgacaacgag ggcgcgccta gaacttggtc ggccagccgc
     1021 ccaggcgatg ccggccgatg gcggagcgcg tgcgcgcctg cgcgaggaag cggatcacga
     1081 cgtcgcgcgt gtcggccggg tcgatcacct cgtcgatgag gtgccgcgcg gccgcgccgt
     1141 aggggagcgt gtcgagctcc cactgcgcga gcagctcctg ccgccgcgcc acgcgctcct
     1201 ccgccggcag cttcgccagc tccgcgccgt gcaccacgtt gacgccggtg gccgggtcca
     1261 tgaaggacat ctccgcggtc ggccaggcca ccagcaggtc cgcgtagcca ccgccgccca
     1321 tgttgaagta ggcctggccg taggtcttgc gcacgatgat ggacacgcgc ggcaccgtga
     1381 ccatgccgag cgcctccatc caggtgatga tcttgccggc cacgcgcttg cgctcggcgt
     1441 cgcggccgat gaagaagccg gggacgtcgt gcaggaacac gagcgggatg ttgaacgagt
     1501 cgcagagcgc gaggaacgag atggccttgt cgcagccgtc ggcgtcgcag gcgccgccct
     1561 tctggatggg ctggttggcc acgaagccca ccacgtggcc gtccaggcgg gcgagcgcgg
     1621 tgatcaggct ccggccgaag cgctccttga cctggaagat ctcgccgccg tcggccagcc
     1681 gccgcagcac ccgcgtcatg tcgtaggcgc ggttgcgcgc ctccggcacc aggtccgcga
     1741 tcgtgctcat ctcggcgccc gagcccgccg gcacctccgc ctgcggcggc gcctgccggt
     1801 tgtgcgaggg cagataggag aggaagcgct tcacgaacgc gaagcactcg tcctccgtct
     1861 tgccgacgac gtcggcgaag ccggtctcct cggcgtgcgc cttcgcgccg cccagctcct
     1921 cggccgagat cttctccccg gtggccagct cgagcacgcg cggacccgag accgccatcg
     1981 aggcgccttc gagcatcacc acgaagtcgg agaggcaggc gttccacgtg ggcagcccgt
     2041 aggcctgccc gagcaccgcg gtgaccatgg gcacacggcg ccggcgcgta ccgtagtagg
     2101 tggagttgcc gaaggaggcg atgcccgcgg agcccatgat gtccggcatg cgcgcgccgc
     2161 cggcctcggc caggtagacg atcggcatgc cccggcgcgc ggcgaggcgc ttgagctccg
     2221 cctccttgtg cgaggccacg cggctcgacg tggcggccag caccgtgaag tcgttggagc
     2281 agaccaccac cgggcggccg tcgatgcggc cgagaccgcc caccttggag tcggccggcg
     2341 tctcggcctc catgcccggg acgtcggagg tgttgaacgt gccgacttcc aggaaggagc
     2401 cggcatcgag caatcgctcc acccgctcgc gcgcgttcag cgtgcccgtg tcccggcggc
     2461 gcgccagccg ctcgggcccg cccatcgcgc gcgccttcgc gcgccgggcg tggaaatcct
     2521 tcatccgctc gtcccaggcc atggcggggc attgtagcag cccctcgcca ttccggaagc
     2581 gcttcggtac actgcggggg cacgatcacc gccacgccat cggaggccgc aacgcgatga
     2641 agcaccacaa ggtctaccgc accgagctca cgcccgtgag cttcttggtc cgcagcgcct
     2701 acgtcttccc cgacaagacg gcggtggtgc acggggaacg gcgctacacc taccgtcagc
     2761 tcggcgagcg ggtgaaccgg ctggcgtccg cgctgcgggg cgcgggcatg cagaagcacg
     2821 atcgcgtggc cttcctctgc ccgaacatcc cggccatgct cgaggcgcac tacggcgtgc
     2881 ccgcggcggg cggcgtgctg gtggcgatca acacgcggct ctcctccgac gagatcggct
     2941 acatcctccg gcactcgggc tcgacgttcc tgttcgtgga cgcggagctc gagcacctga
     3001 tcaagccgct cgacctcacg ggcacgcggg tcgttcgcat agacgacacc ggcgcggccg
     3061 gcgatcccta cgagggcttc ctggccggcg gctcgcccga tcccgtcgag agctggctcg
     3121 aggacgagga ggagacgatc tcgatcaact acacctcggg caccacggga cggcccaagg
     3181 gcgtgatgta ctcacaccgc ggcgtctggg tgaacgcggt cggcgaggtc gtcgagaccg
     3241 gcatgacctt cgacacgaag tacctctgga cgctgcccat gtttcactgc aatggctggt
     3301 gcttcacctg ggcggtcacc tcggtcgcgg gcacccacgt gtgtctccgg agagtcgagc
     3361 ccgcgcgcat ctgggagctg atcgacgccg agggcatcac gcactacaat ggcgcgccca
     3421 ccgtgcagat cggggtggtc aacgacccga aggcgcacaa gctggcgcgg ccggtgacgg
     3481 tgacggtggc cggcgcgccg ccctcgccga cgctgctcgg caagctcaag gagctcggct
     3541 tcaagccggt gcacgtctac ggcctcaccg agacctacgg gccgcacacc gtgtgcggct
     3601 ggaacgcgga ctgggacgcg ctgccggccg aggagcaggc gcggctggcc gcccgccagg
     3661 gccagggcta cgcgctcttc gacctcgtgc gcgtggtgga cggcgagatg aacgacgtgc
     3721 cgcgcgacgg cgacaccctg ggcgaggtga tcatggtcgg caacaacgtg gccaagggct
     3781 actacgagca gcccgacgcc accagcgagg ccttccgcgg cggctggttc cactcgggcg
     3841 acatcggggt ctggcatccg gacggctacg tagagctgcg cgaccgcaag aaggacatca
     3901 tcatctcggg cggcgagaac atctcgacca tcgaggtcga gcagtgcgtg gcccggcacc
     3961 cggcggtgat ggagtgcgcc gtcgtcgcca tccccgacga gaagtggggc gagcggccca
     4021 aggccttcgt cacgctcaag ccggggcagc gcgcgaccga gcccgagatc atcgagttct
     4081 gcaagcagca catcgcgggc ttcaaggcgc ccgccgccgt cgagttcggg gagctgccca
     4141 agacctcgac cgggaagatc cagaagttcg tgctgcgcga ccgcgagtgg aaggggcggg
     4201 ccaaacggat caactaggca tgcgcgtcgg catcctcgac cagtcgccgg tgcggagcgg
     4261 gggcacgccc gccgacgcgg tccacgagac gatcgagctc gcgcgcgccg ccgaccgctg
     4321 ggggtttcac cgctactggc tggccgagca ccactccacg cccggcctgg ccggcgcgag
     4381 tcccgaggtg ctgatcgccc aggtggccgc ggtcaccagc cacatccggg tgggttcggg
     4441 cggcgtcatg ctgccgcact acagcgccct caaggtggcc gagcagttcc ggatgctgga
     4501 gacgctccat cccggccgca tcgatctcgg gatcgggcgc gcgccgggca gcgatcagcg
     4561 gacggcccgg atcctgcggc acgggcccgg caccctcggg atcgagcact acccgcggca
     4621 gatccaggat ctggtcgcgt tcctccatga cgacctgccg gccgagcacc cctggcgggg
     4681 ggtgcacgcc atgcccgccg ggccgagcgt gccggaggtg tggttgctgg gctcgagcga
     4741 cgagagcgcg ctgctcgccg cccagctcgg ccagtccttc tgcttcgccc acttcatcaa
     4801 cgagcagggc ggggcggagg tcacgcgcgc ctaccgcgag cggttctgcg cctcccccga
     4861 cctggccgcg ccgcgcagca gcgtggccgt cttcgcggtc tgcgcggaga cggaggccca
     4921 ggcccggcgc ctggcgcgga gccgcgatct cttcatcgtg cggctctaca ccgggcggcc
     4981 cgcgccgtac ccgtcggtgg aggaggcgga gagctatccc tacaatgcgc aggaggtggc
     5041 catcgcccag cacgcggccc ggcgcagcgt ggt
//
LOCUS       DASUFA010000317         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_997163, whole genome shotgun sequence.
ACCESSION   DASUFA010000317 DASUFA010000000
VERSION     DASUFA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997163"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFX87_11810"
     CDS             <1..715
                     /locus_tag="VFX87_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4995654.1"
                     /translation="EDDATRVIIVAGAGAHFSAGHDLGSPDELEDQKAHPVGPGLPEN
                     LKRLSELYLETCLRWREVPKPTIAQVQGYCIMGGLMLASCCDLIIAAEDALFADRAVR
                     WGGSHVQYFSMPWDLGPRKAKEYLFTGDYIDASTALELGLVNRVVPRAELADATLELA
                     GRIALQDPFALKLAKASVNETMDIQGQRRALEAAFKNYMLTIPHRQALGTYGTAARAQ
                     GVKDRIKTRDVRFGDAAGG"
     gene            complement(755..2518)
                     /locus_tag="VFX87_11815"
     CDS             complement(755..2518)
                     /locus_tag="VFX87_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4995655.1"
                     /translation="MRAAALVVAGDAAYGMGAYRLAAERYSEALLSDELPPNAAHATL
                     ALGWAELRLGRREDARLTWMRVARQFPSDPAAPIALILAAELSAQTGEMVVARKLLDR
                     ALEGHPVTPEAQIARLSRSILAMRDGRTQEAARDLRMLARSGGPSVPVERRKLLDGLM
                     ATGTQAGPEHQMLLTNQYVAGQPGLPSTDGRPQRDESATAPDTAGMLERFAAPFLDGA
                     GDPETTPRVLHGLLLVAVEDKAWPEVQTLAGRLVDRFPGYTPAPELLVQVAGQAAAEQ
                     QWPVVRTSYEYATARSRPTVLAPQAQVDFAEALFRTGNAAQARVELTRFVDTAPRAQE
                     APRALHLLAEVSETLDEPREALAAYERLRRDFPRAERTTQSLLSHARLLQYAVGQEKQ
                     AQALLEDIVQRTEGEERAEASFRLAQVLAGEGKHPSAVDWYMAAAYGTAEHSRWYRSA
                     LLGAGHSLVALKRSRAALAVYRNVLPSTPIGPLPRDGRPAPEPIAQVEEPELVAEAAY
                     QIAQISRAAGRNEEAVDMYLTAAYLAPGSQGRALMGAVRSLVAVGDRASAEAVFRRLV
                     ESSGEEPEILTQASKVFRRGR"
     gene            complement(2626..>2861)
                     /locus_tag="VFX87_11820"
     CDS             complement(2626..>2861)
                     /locus_tag="VFX87_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995656.1"
                     /translation="VRPVVVARPVVVARPVVVARPVVVARPVVVARPVVVARPVVMAR
                     ALVRVRAIAGAAVVLVAGIPAQVPELLVRAPGG"
BASE COUNT          401 a         1073 c         1011 g          376 t
ORIGIN      
        1 ggaggacgac gcgacgcgcg tcatcatcgt ggcgggcgcc ggggcgcact tctcggcggg
       61 ccacgacctc ggcagcccgg acgagctcga ggaccagaag gcccacccgg tcggcccggg
      121 cctgcccgag aacctcaagc ggctgtccga gctgtacctc gagacctgtc tgcgctggcg
      181 cgaggtgccc aagccaacca tcgcccaggt gcaggggtac tgcatcatgg gcgggctcat
      241 gctggcctcg tgctgcgacc tcatcatcgc cgccgaggac gcgctgttcg ccgaccgggc
      301 ggtgcgctgg ggcggctcgc atgtgcagta cttcagcatg ccgtgggatc tcgggccgcg
      361 gaaggccaag gagtacctct tcaccggcga ctacatcgac gcgtccacgg cgctcgagct
      421 cggcctcgtc aaccgggtgg tgccgcgcgc cgagctggcc gacgccacgc tcgagctggc
      481 ggggcgcatc gcgctgcagg atcccttcgc cctcaagctc gccaaggcgt cggtgaacga
      541 gacgatggac atccagggcc agcgccgtgc cctcgaggcg gcgttcaaga actacatgct
      601 gaccatcccc caccgccagg cgctcggcac ctacggcacg gcggcgcggg cccagggcgt
      661 gaaggaccgg atcaagacgc gggacgtgag gttcggggac gccgcgggcg ggtgaccgcg
      721 aaccctggtt cgcttcgggc agctcggtcg cagcctaccg cccgcgacgg aacaccttgc
      781 tcgcctgggt gaggatctcg ggctcctcgc cgctcgattc cacgagccgg cggaagaccg
      841 cctccgccga cgcccggtcg ccgaccgcga cgagcgatcg taccgccccc atgagcgcgc
      901 gcccttgtga tccgggcgcc aggtaggcgg cggtcaggta catgtcgacg gcttcctcgt
      961 tgcgaccggc cgcccggctg atctgcgcga tctgataggc ggcctcggcc accagctcgg
     1021 gctcctcgac ctgcgcgatc ggctccgggg cagggcggcc gtctctcggc aagggcccga
     1081 tcggggtcga cggcaggacg ttgcgataca ccgcgagcgc ggcccgcgag cgcttcagcg
     1141 cgaccagcga gtggccggcg cccagcaagg cggaccgata ccatcgcgag tgctcggcgg
     1201 tgccgtacgc ggcggccatg taccagtcca ccgcgctcgg atgcttgccc tcgccggcca
     1261 ggacctgggc gagtctgaac gacgcctcgg cgcgctcctc gccctcggtg cgctgcacga
     1321 tgtcctccag caaggcctgg gcctgtttct cctgtccgac ggcgtactgg agcagacggg
     1381 catgggagag gaggctctga gtggtccgct cggcccgcgg gaagtcgcga cgcaggcgct
     1441 cgtacgcggc cagggcctca cgtggctcgt ccagcgtctc gctaacctcg gccagcagat
     1501 gcagcgcacg gggcgcttcc tgcgcgcgag gcgcggtgtc cacgaagcgg gtgagctcga
     1561 cgcgggcctg ggccgcgttg cccgtccgga acagcgcctc ggcgaagtcg acctgggcct
     1621 gcggggccaa gacggtgggc cgggagcggg ccgtcgcgta ctcgtagctc gtccggacga
     1681 cgggccactg ctgctcggcg gcggcctggc cagccacctg gaccagcagc tcgggcgccg
     1741 gggtgtagcc ggggaatcgg tcgacgagac ggcctgccag cgtctggacc tccggccacg
     1801 ccttgtcttc caccgcgacg aggagcagtc cgtggagcac ccgcggcgtg gtctccggat
     1861 cgccggcgcc gtcgaggaag ggggcggcga accgctcgag catcccggcc gtgtccggcg
     1921 ccgtcgcgct ctcgtcgcgc tgcgggcgtc catcggtcga gggcagaccg ggctgccctg
     1981 ccacatattg attcgtcagg agcatctggt gctccgggcc ggcctgtgtc cccgtggcca
     2041 tgagcccgtc gaggagcttg cggcgctcca ctggtaccga ggggccaccc gatcgagcga
     2101 gcattcgcag gtcccgggct gcttcttgtg tgcgcccgtc ccgcatggcc agaatcgacc
     2161 ggctcagccg cgcgatctgc gcctccgggg taaccgggtg gccctccagc gcccgatcga
     2221 gcagcttgcg agcgaccacc atctcgcccg tctgggcgga gagctccgcg gccaggatga
     2281 gcgcgatggg cgcggccgga tccgacggga actgccgggc gacgcgcatc cacgtcaggc
     2341 gggcatcctc gcgacgcccc aggcgcagct cggcccagcc cagagcaagg gtcgcgtgcg
     2401 ccgcgttggg cggcagctcg tcactcagca gggcctcgct gtagcgctcg gctgccaggc
     2461 gataggcgcc catgccgtac gcggcgtcgc cggcgacgac gagggcggcc gcccgcatgc
     2521 ttggagagac gcgcccaccc agcagaacag gggcgagctg atctgggcgc tccgatggct
     2581 tgggcgcgga cggaggaggc gtccgctttg ccacgccgct gccgtctacc cccccggcgc
     2641 ccggaccagc agctccggca cttgcgccgg aatccccgcc accaggacta ctgccgctcc
     2701 cgctatcgcc cgcacccgca ccagcgcccg cgccatcacc accggccgcg ccaccaccac
     2761 cggccgcgcc accaccaccg gccgcgccac caccaccggc cgcgccacca ccaccggccg
     2821 cgccaccacc accggccgcg ccaccaccac cggccgcacc a
//
LOCUS       DASUFA010000318         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1117082, whole genome shotgun sequence.
ACCESSION   DASUFA010000318 DASUFA010000000
VERSION     DASUFA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117082"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2312)
                     /locus_tag="VFX87_11825"
     CDS             complement(105..2312)
                     /locus_tag="VFX87_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995657.1"
                     /translation="MFARVLALGVALVAGASPARAGHELPFYPGYYPQEIRIEALAPA
                     AAVPLLRKGALHAYVGGDPFAGGRLPAHITSVDSLGGYLVGRFNPAPPEALARCAAAA
                     RAGKSLGGPRAGQVAHPYAVTPYHADYLQHWDLAQAARKAVEAAPAGPPSRPVAVEEV
                     DLQSLLATYAIGAGGSFAPPWLKEGWFHAYLLQAPAITDPAARQAVEGLYRRLVTFAY
                     DGAAERADLERRLVQRLAAGCERPVLGYTVRREPFNSEFSQGIENIAWDSQRGFNSDI
                     FVRTVKLKDFPWNGWLKVGVAGRATAAWNPVGGFTDAPGRLLWAAVGDPGLIPAPYAA
                     GVVPHRAVPAQVAVAGPSGFEIPEDALLPESGTGALLPVGKGKTAKARVTYRLWGSAA
                     HDNTRVSAADALYSYVFAAHWSVRRAPGGVEYDPAVEAATETARQALAGVRVVKVDSE
                     VRRYSDITFTYVVPVIEVYLNAGSADTAELAALAPPWSATPWHVMVLMEEAVKRGVGA
                     FSAAEARRRGVRWLDLARDPKTREELGAILDGLARANHVPEALKRLVAADEAQARWAA
                     LRQFAQRRGHYLVTNGPYQLEKWTDGAVVLQVFRDMNNPLGVGSYDRFAIPRRAYVAR
                     ITARGDRLEIAPEIERLEKFLREYRLVREPLGSRGEEERPDLPACRYVILGANGTVAA
                     AGVSREVQAHRLIVDLKGRLKPGAYRALVALSLGDNLVDPEVATAQFRVDAGP"
     gene            complement(2420..3805)
                     /locus_tag="VFX87_11830"
     CDS             complement(2420..3805)
                     /locus_tag="VFX87_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995658.1"
                     /translation="MTQEVSRRDFLARLGVTVGTAAAGAATAAGVPLAAVPLISQAHA
                     QPKGSIPDTPYKIAHMTFFTGAAAVLGEPSFKGHTLAAEEINAQGGLLGKRKIESFKA
                     DENAGTDANVKEMRRLKLSEKVDLFTGVISSGNTPALGPVAEELKLLTIFVDGCTDFL
                     FDKAVPNPHYIFRITNIQSADGVTCAVAAAMTWGKQIKRIAHIHPDYSYGRNAFDHFN
                     IVMKKMVPGAQVVSEGWPKLGTTDFTSHITKAISSKPDLLVSSVWGGDYVAMYKQALR
                     YDMFKKMKFASTIAFGVAPHAIGKDHPEGAIAGVHSNYYFNYPPNNRWPLNTEFVKKY
                     FDRWKEYPNFQSEGAYSTLYMLKTAVEKANKLMGGWPDDDAIISQLEGMMHAGPAGYV
                     YIRPDTHQGYKDAMTGFSINSPDYPFQILDPTRIITIPIRNITAPPGWPKGEPTSTYT
                     WIDKTWPQMKA"
     gene            3947..4672
                     /locus_tag="VFX87_11835"
     CDS             3947..4672
                     /locus_tag="VFX87_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995659.1"
                     /translation="MGGARRILTGIGRALRARPRLFVGVAAAVLALDILVPVAVLSIA
                     RVPVDYFTFNPWLGNLPGYLASGRGSLAERLGRAWDLALFWFSANGVFGIDWGFAVTT
                     ADLARFILMAALVGVYFALWVHRRAGGGAAGWPMRAGGQGGVLGAAGSVCGLATGGCT
                     VMGCGAPVIPVVGLAFVGLSSTTLTLFAQVSTYGTLAVISGLTAGVLYLAWRVGNAPP
                     HPALAPSGGVGQDSRSPLGRGPG"
BASE COUNT          653 a         1620 c         1717 g          725 t
ORIGIN      
        1 caagctcagc ccgcccgcga tcaggaacat caccgacgcg tagaggagcg cgttgaggac
       61 gtggacgacc agggtgtcca tgggccccga taagatgccc tagttcatgg gccggcatca
      121 acccggaact gcgcggtggc cacctccggg tcgacgagat tgtcgcccag ggacagcgcc
      181 accagggccc tataggcgcc cggcttgagc cggcccttga gatccacgat cagccgatgg
      241 gcctgcacct cgcgggacac cccggcggcg gccaccgtgc cattggcgcc gaggatcacg
      301 tagcgacagg cgggcagatc gggccgctcc tcctcgcctc gggagccgag cggctcgcgg
      361 acgaggcggt attcccggag aaacttctcc agccgctcga tctccggcgc gatctcgagc
      421 cgatcgccgc gcgcggtgat gcgtgcaacg taggcacgcc gcgggatggc gaagcgatcg
      481 taggacccga cgccgagcgg gttgttcatg tcccggaata cctgcagcac gaccgccccg
      541 tcggtccact tctcgagctg gtagggccca ttcgtgacca ggtagtgacc ccggcgctgg
      601 gcgaactggc gcagcgcggc ccagcgggcc tgcgcctcgt ccgcggccac cagccgtttg
      661 agcgcctccg gcacgtggtt ggcgcgcgcg agcccgtcca ggatcgcgcc gagctcctcg
      721 cgggtcttcg ggtcgcgcgc gaggtcgagc cagcgcaccc cgcgtcggcg ggcctccgca
      781 gccgagaagg cgccgacgcc ccgcttgacc gcttcctcca tcagcaccat cacgtgccag
      841 ggcgtcgcgc tccagggcgg cgccagcgcg gccagctccg cggtgtccgc gctgcccgcg
      901 ttgaggtaga cctcgatcac cggcaccacg taggtgaagg tgatgtcgct gtagcgccgc
      961 acctcgctgt ccaccttcac cacccgcaca ccggcgaggg cctggcgcgc ggtctcggtg
     1021 gcggcctcga ccgccgggtc gtactcgacg ccgccgggcg cgcgccggac gctccagtgc
     1081 gcggcgaaga cgtaggaata gagcgcgtcg gccgccgaga cgcgcgtgtt gtcgtgggcc
     1141 gccgatcccc agaggcgata ggtcacgcgg gccttggcgg tcttcccctt ccccaccggc
     1201 agcagcgcgc cggtgcccga ctccggcagc agcgcgtcct cggggatctc gaagcccgac
     1261 gggcccgcga ccgcgacctg ggcgggcacc gcgcgatgcg ggacgacgcc ggcggcgtag
     1321 ggcgccggga tcagaccggg gtcgccgacc gcggcccaga gcagccgccc gggcgcgtcg
     1381 gtgaacccgc cgaccggatt ccaggcagcg gtggcgcggc ccgccacgcc gaccttcagc
     1441 cagccgttcc atgggaaatc cttgagcttg accgtgcgca cgaagatgtc ggagttgaag
     1501 ccccgctggg aatcccaggc gatgttctcg atgccctgcg agaactcgga gttgaacggc
     1561 tcacgccgca cggtatagcc gagcacaggg cgctcgcacc cggccgcgag ccgctggacg
     1621 aggcggcgct cgagatccgc gcgctccgcc gccccgtcgt aggcgaacgt gacgaggcgc
     1681 cggtagagcc cctcgacggc ctggcgggcg gccgggtccg tgatggcggg cgcctggagc
     1741 aggtaggcgt ggaaccagcc ctccttgagc caggggggcg cgaagctgcc gcccgcgccg
     1801 atcgcgtacg tggcgagcag gctctggaga tccacctcct cgacggccac cggtcgggac
     1861 ggcgggcccg cgggcgcggc ctccacggcc ttgcgggcgg cctgggcgag gtcccagtgc
     1921 tggaggtagt cggcgtgata gggcgtgacc gcgtagggat gtgcgacctg gccggcgcgc
     1981 ggcccgccca aactcttgcc ggcgcgcgcg gcggcggcgc agcgggccag ggcctcgggc
     2041 ggagccgggt tgaagcgccc gaccaggtag cccccgagcg agtccacgga ggtgatgtgg
     2101 gcgggaagcc ggccgcccgc gaagggatcg ccaccgacat aggcgtggag cgcgcccttc
     2161 cggaggagcg gcaccgcggc ggccggggcc agcgcctcga tgcggatctc ctgcgggtag
     2221 tagccgggat agaagggcag ctcgtgcccc gcgcgcgcgg gagacgcgcc ggcgacgagc
     2281 gccacgccga gggcgaggac gcgagcgaac acacgaaccc cctcaccccg gcccgctccc
     2341 ggcggggggg aggaggcagg aaaactccct ctcccccggg agggggagag ggccgggtga
     2401 gggggagtgt ccgtgcgccc taggccttca tctgaggcca ggtcttgtcg atccaggtgt
     2461 aggtagaggt cggctccccc ttcggccagc cgggaggcgc cgtgatgttg cggatcggga
     2521 tcgtgatgat gcgggtcgga tccaggatct ggaacggata gtcgggcgag ttgatgctga
     2581 agcccgtcat cgcgtccttg tagccctggt gcgtgtcggg ccggatgtac acgtagccgg
     2641 cgggtccggc gtgcatcatg ccctcgagct ggctgatgat cgcgtcgtcg tccggccagc
     2701 cgcccatcag cttgttcgcc ttctcgaccg cggtcttcag catgtagagg gtggagtacg
     2761 ctccctcgga ctggaagttc ggatactcct tccaccggtc gaagtacttc ttcacgaact
     2821 cggtgttgag cggccagcga ttgttcgggg ggtagttgaa gtagtagttg gagtgtacgc
     2881 cggcgatcgc gccttccgga tgatccttgc cgatcgcgtg cggggctacc ccgaaggcga
     2941 tcgtggaggc gaacttcatc ttcttgaaca tatcgtagcg cagcgcctgc ttgtacatgg
     3001 ccacgtagtc gccgccccac accgaggaca ccagcaggtc cggcttcgac gagatggcct
     3061 tggtgatgtg cgacgtgaag tcggtcgtgc cgagcttggg ccagccctcc gacaccacct
     3121 gggcccccgg caccatcttc ttcatcacga tgttgaagtg gtcgaaggca ttgcgcccgt
     3181 aggagtagtc ggggtggatg tgggcgatcc gcttgatctg cttgccccac gtcatggccg
     3241 cggccaccgc gcaggtgacg ccgtcggccg actggatatt cgtgatgcgg aagatgtagt
     3301 gcgggttcgg caccgccttg tcgaacagga agtcggtgca cccgtccaca aagatcgtga
     3361 gcagcttcag ctcctcggcc accgggccga gcgcgggggt gttgccgctc gagatcaccc
     3421 cggtgaagag gtcgaccttc tcggacagct tgagccgccg catctccttc acgttggcgt
     3481 cggtgcccgc gttctcgtcg gccttgaacg actcgatctt ccgcttcccg agcaggccgc
     3541 cctgggcgtt gatctcctcg gcggcgagcg tgtgcccctt gaaggagggc tcccccagca
     3601 ccgcggcggc gccggtgaag aaggtcatgt gagcgatctt gtacggcgtg tccgggatac
     3661 tgcccttggg ctgcgcgtgc gcttgcgaga tcagcggcac ggcggccaag ggcacgccgg
     3721 cggccgtggc ggcgccggcg gcggcggtgc cgaccgtcac gcccagacgg gccaggaaat
     3781 ctcgacgact cacctcttgc gtcatgggtg ctccctcctc atggagaggc cggttaagga
     3841 cagtcgggca gggctcggac ggatcatccg cagccatact gccccagcag ccgatgcaac
     3901 cgcaaggatc tcgcgcaccg gctataatcg ggccggggtc cgggccatgg gcggggcgag
     3961 acgaatcctg acgggaatcg gccgggcgct gcgcgcgcgg ccccggctct tcgtcggcgt
     4021 ggccgccgcg gtcctggccc tggacatcct ggtcccggtc gcggtcctct cgatcgcccg
     4081 cgtccccgtg gactacttca ccttcaaccc ctggctcggg aacctgccgg gctacctggc
     4141 ctccggcagg ggctcgctcg ccgagcgcct gggccgggcc tgggacctgg cgctcttctg
     4201 gttctcggcc aacggcgtct tcggcatcga ctggggcttc gcggtgacca ccgccgacct
     4261 ggcccgcttc atcctgatgg ccgcgctggt cggcgtctac ttcgcgctct gggtgcaccg
     4321 gcgcgccggc ggcggcgcgg cgggctggcc gatgcgcgcg ggtgggcagg gcggggtgct
     4381 gggcgcggcg ggtagcgtgt gcggcctggc caccggcggc tgcaccgtca tgggctgcgg
     4441 cgcgccggtg atcccggtgg tgggcctggc cttcgtcggg ctctcgagca ccaccctcac
     4501 cctgttcgcg caggtctcga cctacgggac gctcgcggtg atttcggggc tgaccgcggg
     4561 cgtgttgtac ctcgcgtggc gcgtcgggaa cgcgccccct caccccgccc tcgccccctc
     4621 cgggggcgtg ggtcaagact cccgctcccc tctggggaga gggccggggt gaggggtccc
     4681 gagttctagc gccccttgaa ctggggctcg cgctt
//
LOCUS       DASUFA010000319         5721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_753811, whole genome shotgun sequence.
ACCESSION   DASUFA010000319 DASUFA010000000
VERSION     DASUFA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753811"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1270
                     /locus_tag="VFX87_11840"
     CDS             107..1270
                     /locus_tag="VFX87_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX4995660.1"
                     /translation="MAVDPILMEPVGLIPVAWPVSQDAPADTFGQPIQVQETEVAPPV
                     QAVVSLPLVTEPTPAIARPAPAPAYPVQFNEHVRFFLDRFQTGYRRAITEGRLARSGR
                     YLPMIMDIFKEKGLPEELVFTAMIESGFNPVAVSRAGAKGLWQFMAPTARLYGLRVDR
                     WLDERLDPEKSTVAAANYLRDLYTVFGSWDLAQAAYNAGEMRVQQAIQGSGTRDFWVL
                     HRRSPYLLPETKNFVPAIHAATLIGRQPEQYGFTVVPEEPERYDVVTVPKGSRLMRLA
                     GLSGVALADLEQLNPELWQKQTPPDAPYELKIPLGTAAAMQAAVELDTASRRVASRVP
                     STPRAGVYVVQAGDTVWRIARQHGVSAVQLARWNGLEHPDRIFPGERLRVTAS"
     gene            1353..2204
                     /locus_tag="VFX87_11845"
     CDS             1353..2204
                     /locus_tag="VFX87_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995661.1"
                     /translation="MGRLDGKVVVVTGASRGIGADIARLFASEGGRVICAARTLREGQ
                     HPLAGSLETTVAAIREAGGEAHAVAANIAEPAECERLIEEAHRRCGPVDVLVNNAALT
                     YYIPVKDYPVGKWLRSWAVNFQAPFVLSQLVLGDMIPRRRGAIVNISSGSAIGPGRGP
                     YPDPTAGARGGTCYGAEKAALERFTQGLASEVFQYGISVTSVAPSQVVPTPGTVYHRL
                     VTGLDDPRGEPPLLMARAALLLATEPVERVSGRVTYSQQILREFGWIETGRGRGVDTR
                     GSGYSEI"
     gene            2208..3185
                     /locus_tag="VFX87_11850"
     CDS             2208..3185
                     /locus_tag="VFX87_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995662.1"
                     /translation="MRRRAFVTMLGGAAASWATVTSAQPRVRLGYLSGGSKGDNEENS
                     VAILKESLRRLGWRLGETLEIEERWASGDFARLPRLAQELVAGRPDILVSTGQTETRT
                     LQAVAGAIPIIFMQVSVDPVIAGFVKQIARPGGNITGFMQSPHFLWGKRIELLTELLG
                     RPPRRLAWIGNPGNTGSEANWADARDAAAKVGSEVIRVNVSTVGDLDRAFDALKSRDA
                     LLVQFDFLLAVQKQRVAAHVARVRLPAIYENRSQATAGGLMSYGGDLRENYRQGAQYV
                     HRVLNGTPPGDLPVVQASRFELVLNVKTARALGLTVPDALLARADEVIE"
     gene            3304..4350
                     /locus_tag="VFX87_11855"
     CDS             3304..4350
                     /locus_tag="VFX87_11855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995663.1"
                     /translation="MHFGIFLEERRRGVSEGATLRETIELADAAEAGGLDGVWLGEIH
                     FNGNRSIQSAPLALASFIAARTRRLRVGIAVQVLPLGNPLRIAEEAATVDQLSEGRLD
                     FGIGRSGSARTYDLLGVPYGESQARFLESLEVIRLAWKGKPFSYDGKFYRVRDITVSP
                     TPYQVPHPPLRMAANSPETFPQVARLGLPLFIGLRDLDIPLLRGHLKTYREAWRESGQ
                     AGEADVYLRIPVYAAPSEAAAREEPREAMTYFFQRHIELIRAGLGRTDTGPGDRRQVM
                     ADRIATMPYDELLKTRVAFGTAPSLRERLGEIAEDLGLSGIVAELNPSGLLSLDQMRR
                     TLDILTREVIPTFR"
     gene            complement(4337..5101)
                     /locus_tag="VFX87_11860"
     CDS             complement(4337..5101)
                     /locus_tag="VFX87_11860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4995664.1"
                     /translation="MCVDDDSFPPIPPISGGAAGSRDFHLTASDGTRVMAHAARAAKP
                     TGAGMVVIPDVRGLHPYYKELADRFAEIGVDAVAIDFFARTAPSEDRSEAFDFMSQVP
                     LTKPETLQADIAAAAAYLRTADGGQVKSLYSVGFCFGGALSYLQAASGLGYAGVIGFY
                     GWPSGLKRWPERPKPVDAVPRYKCPVQSIFGGADEGIPPADVEAFDAACRKAGIKHDM
                     KVYPGAPHSFFDRKYTEFAEASADAWKRVQAFVSGK"
     gene            complement(5130..>5721)
                     /locus_tag="VFX87_11865"
     CDS             complement(5130..>5721)
                     /locus_tag="VFX87_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4995665.1"
                     /translation="GDANDCRRTIDEVIAAHGRLDILVNNAGITIDKTVLKLSDEDWF
                     KVLNVNLSGAFFLSQAALRHMAERGSGRIVNVSSVIGEMGNIGQANYAASKSGLFGLT
                     KTLAREAAFLLAKAGRLADPDGVGLTINTVTPGFIGTEMLEHIPPKVLERIVGQIPLG
                     RLGKPEEVARVVHFLAADASSYITGQVWGVNGGLDM"
BASE COUNT          852 a         2039 c         1988 g          842 t
ORIGIN      
        1 catggctaga acaacgcgga ccttgctaac cggcagtgcg cttctcctgg ccggctgtct
       61 ggccctcgcc ggaccggctc gagccgagac tgactctccc attgcgatgg ccgtggaccc
      121 gatcctgatg gagccggtcg ggctgatccc ggtcgcctgg ccggtatctc aggatgcccc
      181 ggccgacacc ttcggccagc caatccaggt ccaagaaacc gaggtggctc cgccggttca
      241 ggccgtcgtc agcttgccgc tggtgaccga gccgaccccg gcgatcgccc gcccggctcc
      301 ggcaccggcg tatccagtcc agttcaatga gcacgtcagg ttcttcctcg atcgcttcca
      361 gaccggctac cggcgcgcga tcaccgaggg ccgcctcgcg cgatccggac gctatctacc
      421 tatgatcatg gacatcttca aggagaaggg tctcccggag gagctggtct tcaccgccat
      481 gatcgagagc ggcttcaacc ccgtggccgt ctcccgggcc ggcgccaagg ggctgtggca
      541 gttcatggcc ccaaccgcgc gcctgtacgg cctccgggtg gaccgctggc tggacgagcg
      601 gctcgacccc gagaagtcca cggtggccgc ggccaactac ctgcgcgatc tctacaccgt
      661 cttcggctcg tgggacctgg ctcaggccgc ctacaacgcg ggcgagatgc gggttcagca
      721 ggcgatccag ggctcgggca cgcgggactt ctgggtgctc caccgccgca gcccgtacct
      781 gttgcccgag acgaagaatt tcgttcccgc catccacgcc gcgaccctga tcggccgcca
      841 gcccgagcag tacggcttca cggtggtgcc ggaggagccg gagcgctacg acgtcgtgac
      901 ggtccccaag ggcagccggc tcatgcggct cgcggggctc tcgggcgtcg ctctcgcaga
      961 cctcgagcag ctgaatccgg agctctggca gaagcagacg ccgcccgatg ccccctacga
     1021 gctcaagatc ccgctcggca ccgccgccgc gatgcaggcc gcggtcgagc tcgacacggc
     1081 ctcgcgccgg gtcgcgtccc gggtcccatc caccccgcgg gccggcgtct atgtcgtcca
     1141 ggcgggcgac acggtctggc gcatcgcccg gcagcacgga gtgtccgccg tgcagctcgc
     1201 gcgctggaac ggcctcgagc accccgatcg catcttcccg ggcgagcgcc tgcgcgtcac
     1261 cgcgtcctag tcgggcctgc ggccggtcgg ccggctcccg agccggttga ccgcgcgcgg
     1321 ccgccgtgga gaataacggc ccggaggatc tcatgggtcg actcgacggc aaggtcgtgg
     1381 tggtcaccgg ggcgagccgg ggcatcggcg ccgacatcgc ccggctcttc gcatccgaag
     1441 gcggccgcgt gatctgcgcg gcccgcaccc tgcgggaagg ccagcatccc ctcgccggct
     1501 cgctcgagac cacggtggcc gccatccgcg aggcgggcgg cgaggcccac gcggtggcgg
     1561 cgaacatcgc ggagcccgcg gagtgcgagc ggctgatcga ggaggcgcac cggcgctgcg
     1621 gcccggtgga cgtgctggtc aacaacgcgg cgctgaccta ctacatcccg gtgaaggact
     1681 acccggtcgg caagtggctg cgctcgtggg cggtgaactt ccaggccccg ttcgtgctga
     1741 gccagctcgt gctcggtgac atgatcccgc gccggcgcgg cgccatcgtc aatatctcct
     1801 ccggctccgc gatcggcccc gggcgcgggc cctaccccga tcccacggcg ggtgcgcgcg
     1861 gcggcacctg ctacggcgcc gagaaggccg cgctggagcg gttcacccag gggctcgcct
     1921 cggaggtctt ccagtacggg atctcggtga cgtcggtcgc gccgtcgcag gtcgtgccca
     1981 cccccggcac cgtctaccac cggctcgtga ccggcctcga cgacccgcgc ggcgagccgc
     2041 cgctcctgat ggcgcgcgcc gcactgctcc tggccaccga gccggtcgag cgagtgagcg
     2101 gccgcgtaac ctacagccag caaatcctcc gcgagttcgg ctggatcgaa accggccgcg
     2161 gccggggcgt ggacacgcgc ggcagcggct actcggagat ctaggggatg aggcggcgcg
     2221 cgttcgtcac gatgctcggc ggagccgcgg catcgtgggc gacggtgacg tcggcacagc
     2281 cgcgcgtccg gctgggctac ctatccggag gctcgaaggg cgacaacgaa gagaactcgg
     2341 ttgcgatcct gaaggagagc ctgcgccggc tcggctggcg cctcggggaa acgctggaga
     2401 tcgaggagcg ctgggcgagc ggcgacttcg ccaggctgcc acgcctcgcg caggagctcg
     2461 tggcggggcg gcccgacatc ctcgtctcga ccggccagac cgagaccaga accctgcagg
     2521 ccgtcgccgg agcgatcccg atcatcttca tgcaggtctc ggtcgatccg gtgatcgcgg
     2581 gcttcgtcaa gcagatcgcc cgaccgggcg gtaacatcac cgggttcatg cagagcccgc
     2641 actttctctg gggcaagcgc atcgagctgc tgaccgagct cctgggacgc ccgccccggc
     2701 gcctggcctg gatcggcaac ccgggaaaca cgggcagcga ggccaactgg gccgacgcga
     2761 gagacgcggc cgccaaggtc gggagcgaag tgatccgcgt caacgtcagc acggtgggtg
     2821 atctcgaccg cgcgttcgat gcgctgaaga gtcgggacgc tctgctcgtg cagttcgatt
     2881 tcttgctcgc cgtccagaaa caacgggtcg ccgcgcacgt ggcccgggtc cgtctgcccg
     2941 ccatctacga gaaccgatcc caggcgacgg ccgggggatt gatgtcgtac ggaggagacc
     3001 tgagagagaa ctatcgccag ggggcccagt acgtgcatcg agtcctgaac gggacgccgc
     3061 ccggcgatct cccggtggtc caggcgagcc ggttcgagct cgtgctcaac gtcaagacgg
     3121 ccagggcgct cggcctcacc gtcccggatg cgctcctcgc gcgcgccgac gaggtgatcg
     3181 agtgatccac cgcacgcggc agtggcgcga cggcggcggg acctcggggg cctcgcgcgt
     3241 gggctagtga cttgacgacg gaagccctcc ggcgcaagat gcgcgggcgg agggaccgga
     3301 cccatgcatt tcgggatctt cctggaagag cggcgccgcg gcgtcagcga aggcgccacg
     3361 ctgcgcgaga ccatcgagct ggcggacgca gcggaggcgg gtggcctcga cggcgtctgg
     3421 ctcggcgaga tccacttcaa cggcaatcgc tcgatccagt ccgccccgct ggcgctcgcg
     3481 agcttcatcg ccgcccgcac ccggcgcctc cgggtcggca tcgcggtgca ggtgctgccg
     3541 ctcggcaacc cgctgcgcat cgccgaggaa gccgccaccg tggatcagct cagcgagggg
     3601 cgcctcgact tcgggatcgg ccgcagcggc tccgcgcgca cctacgatct cctcggcgtg
     3661 ccctacgggg agagccaggc gcgcttcctc gaatccctcg aggtcatccg cctggcctgg
     3721 aagggcaagc cgttcagcta cgacggcaag ttctaccggg tgcgggacat cacggtgtct
     3781 ccgacaccgt accaggtgcc gcacccgccg ctccgcatgg ccgccaactc tccggagacc
     3841 ttcccgcagg tcgcgcggct gggcctgccg ctgttcatcg ggctgcgcga tctggacatc
     3901 ccgctgctgc gcgggcacct caagacgtac cgtgaggcgt ggcgcgagtc gggccaggcc
     3961 ggggaggcgg acgtctatct gcgcatcccg gtctacgcgg ccccgagcga ggcggccgcg
     4021 cgggaggagc cgcgcgaggc catgacctac ttcttccagc ggcacatcga gctgatccgg
     4081 gccggtctcg gccgcaccga taccggcccg ggggaccgcc ggcaggtcat ggcggaccgc
     4141 atcgccacga tgccgtacga cgagctcctg aagacgcgcg tcgccttcgg caccgcgccg
     4201 tcgttgcggg agcgcctcgg cgagatcgcg gaggacctgg ggctcagcgg catcgtcgcc
     4261 gagttgaacc ccagcggcct cctctccctc gatcagatgc ggcggacgct cgacatcctc
     4321 acccgcgagg tgatccctac tttccgctga cgaaggcctg cacgcgcttc cacgcgtcgg
     4381 ccgaggcctc cgcgaactcg gtgtacttcc ggtcgaagaa gctgtgcggc gcccccgggt
     4441 acaccttcat gtcgtgcttg atccccgcct tccggcacgc cgcgtcgaag gcctccacgt
     4501 ccgcgggcgg gatgccctcg tcggcgcccc cgaagatcga ctgcaccggg cacttgtagc
     4561 gcggcaccgc gtcgaccggc ttggggcgct ccggccagcg tttgagcccc gacggccagc
     4621 cgtagaagcc gatcaccccc gcatagccga gcccgctcgc ggcctggagg taggacagcg
     4681 cgccgccgaa gcagaagccc accgagtaga gcgacttcac ctgcccgccg tcggcggtgc
     4741 ggagatacgc cgcggcggcg gcgatgtcgg cctgcagcgt ctccggcttg gtcagcggca
     4801 cctgcgacat gaagtcgaag gcctcgctgc gatcctccga cggcgcggtg cgcgcgaaga
     4861 aatcgatcgc caccgcgtcc accccgatct ccgcgaagcg atcggccagc tccttgtagt
     4921 aggggtgcag gccccgcacg tccgggatca cgaccatgcc cgcgcccgtc ggcttggccg
     4981 cgcgggccgc gtgcgccatg acgcgggtgc cgtcgctcgc ggtgagatga aagtcgcgag
     5041 agccggcggc gccgcccgag atcggaggaa tcggaggaaa ggaatcgtcg tccacgcaca
     5101 tgataggggt ctcctgtcgg tgagtttcgc tacatgtcca ggccgccgtt gacgccccag
     5161 acctggccgg tgatgtaact cgaggcgtcg gcggccagga agtgcacgac ccgcgcgacc
     5221 tcttcgggct tgccgaggcg gccgagcggg atctggccga cgatccgctc cagcaccttc
     5281 ggcgggatgt gctcgagcat ctcggtgccg atgaagccgg gggtcaccgt gttgatggtg
     5341 agcccgaccc cgtccggatc cgcgagccgg cccgccttgg cgagcaggaa ggcggcctcg
     5401 cgcgccagcg tcttggtgag gccgaacagg cccgacttcg atgccgcgta gttggcctgg
     5461 ccgatattgc ccatctcgcc gatgacgctc gagacgttca cgatgcgccc gcttccccgc
     5521 tccgccatgt gccgcagcgc ggcctgggac aggaagaacg cgccggagag attgacgttc
     5581 agcaccttga accagtcctc gtcggagagc ttgagcaccg tcttgtcgat ggtgatcccc
     5641 gcgttgttga cgaggatgtc gaggcgcccg tgggccgcga tcacctcgtc gatggtgcgc
     5701 cggcagtcgt tggcgtcgcc c
//
LOCUS       DASUFA010000320         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_407157, whole genome shotgun sequence.
ACCESSION   DASUFA010000320 DASUFA010000000
VERSION     DASUFA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407157"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFX87_11870"
     CDS             complement(<1..633)
                     /locus_tag="VFX87_11870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995666.1"
                     /translation="MTVVDSHAHIFPHWSGSCGLPSREVHLKYLQKTLTRPSAKVYRL
                     SDGAAASGQLLFRPGDNTWAGLRDDIEFRPGRHGRLEFRVDGEDFVVQYMPAGMVELE
                     SRPELLLAQMVVAGVDHCVLQAGFTYGYMNDYNALAQRQYPAKFTGLFHVDEPMADTA
                     HWMAEARRAVEQLGLRGLHYQTDTFARYDFRWWFDDRRFDAFWSLIESFGV"
     gene            complement(630..2165)
                     /locus_tag="VFX87_11875"
     CDS             complement(630..2165)
                     /locus_tag="VFX87_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX4995667.1"
                     /translation="MTSILDIRRYPEARDAFRWDALWELVDGNRERLNLAHECVDRHV
                     GKGTALRIQFADGRREEHDFGALAEWSSRFANLLEAEGVARGDRVAIMLEPSLAFYGA
                     VFGTMKRGAVAVPLFTLFGPEGLALRVGDCRPRLLLVERDAARWRAQFPEVTVVAVDD
                     LADRLRAAPARYVPATAPDDLALFQYTSGTTRALPEAVRHTHRSVVTLMVAALYGVGL
                     RAGDRYFCPSSPAWGHGLWHGTVAPLALGIAVGSYSGKFSPARIVEALDAFRVTNLAA
                     APTVYRMIRESGAAAGRRFAPEKLSFTGEPMDGKTWDYVERTFGRKPCSMYGSTEVGV
                     IIVNYPGFEGFEVRRGALGKAAPGWEVAVVGADGPLPAGELGEIAVRRRGEWFRVKDR
                     GYVDADGYFFHAGRSDDVIISGGWTMSALEIEQALLAHPDVLEAAVIGVPDELRGQIP
                     LACIVAPGKGPGFASELQEFVKDRLSRHEYPRAIEIVAELPKTPAGKIDRKTLRDRER
                     DRA"
     gene            complement(2162..>2621)
                     /locus_tag="VFX87_11880"
     CDS             complement(2162..>2621)
                     /locus_tag="VFX87_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEX4995668.1"
                     /translation="VVKGPLTVTSVVAFVQGWGSLYVRAHGLAFDLFDRHPALGIPND
                     FGVPEPPERVHWDESLARAVGVPGPYDYGPERVAWLGHLVTNWMGNGGFLRRLTVKVL
                     RHNLIGDTTWCRGRVTGKAPGGVVTLEMDGTNQRGEVIASGTAEVVLPTR"
BASE COUNT          403 a          967 c          903 g          348 t
ORIGIN      
        1 cacgccgaag ctttcgatca gggaccagaa ggcgtcgaag cgccggtcgt caaaccacca
       61 ccggaaatcg tagcgggcga aggtgtcggt ctggtagtgc aggccccgca acccgagctg
      121 ctccaccgcc cgccgcgcct ccgccatcca gtgcgcggtg tccgccatcg gctcgtccac
      181 gtgaaagagc ccggtgaact tcgcgggata ctgtcgctgc gcgagcgcgt tgtagtcgtt
      241 catgtacccg taagtgaagc cggcctggag cacgcagtgg tccacgcccg ccaccaccat
      301 ctgcgccagc aggagctcgg gcctggactc cagctcgacc atgcccgccg gcatgtactg
      361 gacgacaaag tcctcgccgt ccacgcggaa ctcgagccgc ccgtggcgcc ccggccggaa
      421 ctcgatgtcg tcgcggagcc cggcccaggt attgtcgccg ggccggaaga ggagctggcc
      481 cgaggcggcg gcgccgtcag agaggcgata gaccttggcg gagggacgcg tgagcgtctt
      541 ctgcaggtat ttcaggtgca cctcgcgcga gggcaagccg caggagccgg accagtgcgg
      601 gaagatgtgg gcgtgggagt cgaccacggt catgcccggt cgcgctcccg gtcgcggagc
      661 gtcttccggt cgatcttgcc ggccggcgtc ttgggcagct ccgccacgat ctcgatggcg
      721 cgcgggtact cgtgccggct caggcggtcc ttcacgaatt cctgcagctc gctcgcgaag
      781 ccggggccct tgcccggtgc cacgatgcac gccaagggga tctgccctcg aagctcgtcg
      841 ggcacgccga tcaccgccgc ctcgagcacg tcggggtggg cgagcagcgc ctgctcgatc
      901 tcgagcgcgc tcatggtcca gccgccggag atgatgacgt cgtcggaccg cccggcgtgg
      961 aagaagtagc cgtccgcgtc cacgtagccg cgatccttca cgcggaacca ttccccgcgg
     1021 cgccgaacgg cgatctcgcc gagctcaccg gcgggcagcg ggccgtcggc gccgaccacc
     1081 gcgacctccc agcccggcgc ggccttgccc agcgcgccgc ggcggacctc gaagccctcg
     1141 aagcccgggt agttcacgat gatcaccccg acctcggtac tgccgtacat gctgcagggc
     1201 tttcgcccga aggtccgctc gacgtagtcc caggtcttgc cgtccatggg ctcgcccgtg
     1261 aaggagagct tctcgggggc gaatcgccgc cccgccgccg cccccgactc ccggatcatc
     1321 cggtacaccg tgggcgcggc ggcgagattc gtcacgcgga aggcgtcgag cgcctccacg
     1381 atccgcgccg ggctgaactt gcccgagtag ctgcccaccg cgatgcccag cgccagcggg
     1441 gccacggtgc cgtgccagag cccatgcccc caggcggggg acgacgggca gaagtagcga
     1501 tcgccggccc gcagccccac cccgtacagc gccgcgacca tcagcgtcac caccgagcgg
     1561 tgcgtgtgcc gcaccgcctc cggcaaggcg cgcgtggtgc ccgacgtgta ctgaaagagc
     1621 gcgagatcgt cgggcgcggt ggccggcacg taccgcgcgg gcgccgcgcg gaggcggtcg
     1681 gccaggtcgt ccaccgcgac cacggtcacc tcggggaact gggcgcgcca gcgcgcggcg
     1741 tcccgctcca ccagcagcag gcgcggccgg caatcgccga cgcgcagcgc caatccctcg
     1801 ggaccgaaga gcgtgaagag cggcaccgcg acggcgccgc gcttcatcgt cccgaacacc
     1861 gccccgtaga aggccagcga cggctccagc atgatggcca cccgatcgcc gcgggccacg
     1921 ccctccgcct cgaggagatt ggcgaagcgg gacgaccact cggcgagcgc cccgaagtca
     1981 tgctcctccc ggcgcccgtc ggcgaactgg atgcgcagcg ccgtgccctt gcccacgtgc
     2041 cggtccacgc actcgtgggc gaggttgagg cgctcgcggt tcccgtccac gagctcccac
     2101 agcgcgtccc agcggaacgc gtcccgcgcc tcgggatagc ggcggatgtc gaggatcgac
     2161 gtcatcgggt ggggagcacc acctcggcgg tgccggaggc gatcacctcg ccccgctggt
     2221 tggtcccgtc catctccagc gtcaccacgc ccccgggcgc cttgccggtc acgcggccgc
     2281 ggcaccaggt ggtgtcgccg atcaggttgt ggcgcagcac cttcacggtc aggcgccgga
     2341 ggaagccgcc attgcccatc cagttcgtca ccaggtggcc gagccaggcc acgcgctccg
     2401 gtccgtagtc gtagggaccg ggcacgccga cggcgcgcgc cagcgactcg tcccagtgca
     2461 cgcgctccgg cggctccggc acgccgaagt cgttcgggat gcccagagcc gggtgtcgat
     2521 cgaagagatc gaaggcgagg ccgtgggcgc ggacatacaa gctgccccag ccttgcacga
     2581 aggcgaccac cgaggtaacg gtgaggggtc ccttcaccac c
//
LOCUS       DASUFA010000321         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_174382, whole genome shotgun sequence.
ACCESSION   DASUFA010000321 DASUFA010000000
VERSION     DASUFA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174382"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFX87_11885"
     CDS             complement(<1..312)
                     /locus_tag="VFX87_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX4995669.1"
                     /translation="MLDPSLLPLLHLTDTAFPAGGYAHSFGLETYCQDGRVTDADSLT
                     DFLVMQLEGAAGPCDATAAAAARRRFVAGDIGGCAALDGTLEAMKPVRELREASRQMG
                     RQ"
     gene            complement(321..764)
                     /locus_tag="VFX87_11890"
     CDS             complement(321..764)
                     /locus_tag="VFX87_11890"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX4995670.1"
                     /translation="MRVITTLPPRVEPAALAGLERDTLSLTWEERRWTRKRVRSTAGR
                     EVALALPTGSLLRPGDVLAREASWYLVVEGRPEPVLAILPRDRDEAIRIAFEVGNRHF
                     SLALDGECILVPDDTAMEQLLIRMGVAWKREETVYDPLGDTAHRH"
     gene            complement(772..2487)
                     /gene="ureC"
                     /locus_tag="VFX87_11895"
     CDS             complement(772..2487)
                     /gene="ureC"
                     /locus_tag="VFX87_11895"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX4995671.1"
                     /translation="MSLRLPRRQYADLYGPTVGDRVRLADTDLLIEVERDFTTYGDEV
                     KFGGGKVIRDGMGQSARATAGEDVLDLVITNALILDHWGIVKADLGIRAGRIVAIGKA
                     GNRDIMAGVTAGMVIGASTEVIAGEGRIVTAGGIDSHIHFICPQLVEEALSAGLTTLI
                     GGGTGPATGTAATTCAPGAWNIHRMLEAAEAFPINLGFLGKGNASRPAPLREQVEAGA
                     IGLKLHEDWGTTPAAIDAALSVAEEMDIQVAIHTDTLNEAGFVEDSIRAFKGRTIHTY
                     HTEGAGGGHAPDIIRVVGEANCLPSSTNPTMPFTVNTMDEHLDMLMVCHHLNAKIPED
                     LAFAESRIRAETIAAEDVLHDLGAISMLSSDSQAMGRIAEVISRTWQTAHKMKTQRGA
                     LPGDDRHDNTRAKRYVAKYTINPAIAHGIAHEVGSIEVGKLADLVIWKPAFFGVKPEM
                     VLKGGLIAVAAMGDPNASIPTPQPVRYRPMFAAYGAAVSSTSLTFLSRAATEDGVAAR
                     LGLRKAIATVRGCRGLTKAAMVHNNYVPRMEVDAETYEVRADGQLLTCEPASVLPLAQ
                     RYFLF"
     gene            complement(2484..2855)
                     /gene="ureB"
                     /locus_tag="VFX87_11900"
     CDS             complement(2484..2855)
                     /gene="ureB"
                     /locus_tag="VFX87_11900"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX4995672.1"
                     /translation="MIPGEYLLGDRDIEANAGRRTLQLTVANTADRPIQVGSHFHFFE
                     ANRGLRFDREKAFGMRLNVPAGTAVRFEPGDEKVVTLVELGGAREVYGLNALTDGSAA
                     APGRAASVKRAKAGGFIEAGS"
BASE COUNT          423 a         1072 c         1002 g          469 t
ORIGIN      
        1 ctggcggccc atctgacggc tcgcctcccg cagctcgcgc acgggcttca tcgcctccag
       61 cgtgccgtca agcgcggcgc agccgccgat gtcccccgcg acaaagcgcc gccgcgcggc
      121 cgccgccgcg gtggcgtcgc agggcccggc cgcgccctcg agctgcatga cgagaaaatc
      181 ggtgagcgag tcggcatccg tcacgcgtcc gtcctgacag tacgtctcca ggccgaagga
      241 atgcgcgtag ccgccggcgg ggaacgccgt gtccgtcagg tgcagcaatg gaaggagaga
      301 agggtcgagc atcacgggcg tcagtgccga tgcgccgtat cgcccagagg gtcgtagacc
      361 gtctcctcgc gcttccacgc cacacccatt cggatgagga gctgctccat ggcagtgtcg
      421 tccggcacca ggatgcactc cccatcgagg gcgagggaaa agtggcggtt ccccacctcg
      481 aaggcgatgc ggatggcctc gtcacggtcc cgcggaagta tcgcgagcac cggctccgga
      541 cgcccctcca ccaccagata ccagctcgct tcccgcgcga gcacgtcgcc cggccgcagc
      601 aggctccccg tcggtagcgc cagcgccacc tcacgtccgg cggtgctccg cacccgcttg
      661 cgggtccagc gccgctcctc ccaggtgagt gacagcgtgt cgcgctcgag tccggccagc
      721 gccgccggct ccacgcgcgg gggcaatgtg gtgatcaccc gcatgcgggg cctagaagag
      781 gaagtatcgc tgcgccagcg gcagcaccga ggcgggctcg caggtgagga gctggccgtc
      841 ggcccgcacc tcgtaagtct ccgcgtccac ctccatgcgg ggcacatagt tgttgtggac
      901 catggccgcc ttggtgagcc cgcggcagcc ccgcacggtg gcgatagcct tccgcaggcc
      961 gaggcgcgcg gccacgccgt cctcggtagc ggcgcgcgag aggaaggtca gcgaggtgga
     1021 cgacaccgcc gcgccgtagg cggcgaacat gggccgatag cgcaccggct ggggcgtggg
     1081 gatggacgcg ttcggatcgc ccatggcggc caccgcgatc agcccgccct tgagcaccat
     1141 ctcgggcttg accccgaaga aggcgggctt ccagatcacg aggtcggcca gcttgcccac
     1201 ctcgatcgag cccacctcgt gcgcgatgcc gtgggcgatc gccgggttga tcgtgtactt
     1261 ggccacgtag cgcttggccc gcgtgttgtc gtggcggtcg tcgccgggga gcgctccgcg
     1321 ctgcgtcttc atcttgtggg cggtctgcca cgtgcgcgag atgacctccg cgatgcgccc
     1381 catcgcctgc gagtcggacg acagcatgct gatcgcgccg aggtcgtgca gcacgtcttc
     1441 cgccgcgatc gtctctgcgc gaatgcgcga ctccgcgaag gccaggtcct cggggatctt
     1501 cgcgttcagg tgatggcaca ccatcagcat gtcgagatgc tcgtccatgg tgttcaccgt
     1561 gaacggcatc gtggggttcg tcgaggacgg caggcagttg gcctcgccga ccacgcggat
     1621 gatgtcgggg gcgtgtcccc cgcccgcgcc ttccgtgtgg tacgtgtgga tcgtccgccc
     1681 cttgaacgcg cggatcgagt cctccacgaa gccggcctcg ttgagcgtgt cggtgtggat
     1741 cgccacctgg atgtccatct cctcggccac cgacagcgcg gcgtcgatcg cggcgggcgt
     1801 cgtcccccag tcctcgtgga gcttgagccc gatcgctccc gcctcgacct gctcccgcag
     1861 cggcgcgggc cgcgaggcat tgcccttgcc gaggaatccc aggttgatcg ggaaggcctc
     1921 cgcggcctcc agcatgcggt ggatgttcca ggctcccggc gcgcaggtgg tggccgcggt
     1981 gccggtcgcg gggccggtgc ccccgccgat cagggtcgtg agcccggccg agagcgcttc
     2041 ctccacgagc tgcgggcaga tgaagtggat gtggctgtcg atgccgccgg cagtcacgat
     2101 acgcccctcg ccggcgatca cctcggtgga ggcgccgatc accatgcccg cggtcacccc
     2161 cgccatgatg tcccggttcc cggccttacc gatggcgacg atgcgcccgg cccggatgcc
     2221 gagatccgcc ttcacgatgc cccagtgatc gaggatcagg gcgttggtga tcaccaggtc
     2281 cagcacgtcc tcgcccgcgg tggcgcgcgc ggactggccc atgccgtcgc ggatcacctt
     2341 gccgcccccg aacttcacct cgtcgccgta ggtggtgaag tcgcgctcga cctcgatcag
     2401 caggtccgta tccgcgagcc gcacccggtc cccgacggtc ggaccgtaga gatcggcgta
     2461 ctgccgccgc ggcagtcgca gactcacgag cccgcctcga tgaagccgcc cgccttcgcg
     2521 cgcttcacgc tcgcggcccg gcccggcgcc gcggcggacc cgtcggtaag cgcgttgagg
     2581 ccgtagacct cgcgcgcccc gcccagctcc accagggtca ccaccttctc gtccccgggc
     2641 tcgaagcgca ccgcggtccc cgccggcacg ttgagccgca tcccgaaggc cttctcgcga
     2701 tcgaaccgca gcccgcggtt cgcctcgaag aaatgaaagt gcgagcccac ctggatcggc
     2761 cgatccgcgg tattcgccac cgtcagctgc agcgtcctcc gccccgcgtt cgcttcgatg
     2821 tcgcggtcgc cgaggaggta ttcgcccggg atcatggagt ctctctcgtt tgggtggaag
     2881 gcgcagcccg ctcttctttc atatgcgggg gcccagaaat ggcccccgca ttggatagag
     2941 gtgcgggtac gtcagccatc accgca
//
LOCUS       DASUFA010000322         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1452111, whole genome shotgun sequence.
ACCESSION   DASUFA010000322 DASUFA010000000
VERSION     DASUFA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452111"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFX87_11905"
     CDS             complement(<1..416)
                     /locus_tag="VFX87_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003593448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX4995673.1"
                     /translation="MNRFVTRTRATWMGAVAAAVVGLVALAPHLGHTNTPAPLWSERA
                     VPATTAAVAPQPNWVELTRALKPAVVNISTKRVEERIQLQTPFGDNDPFQRFFKQFEQ
                     EQPPRRSVRSMGSGFIINPAGYIVTNNHVVEGASDIR"
     gene            complement(526..1926)
                     /locus_tag="VFX87_11910"
     CDS             complement(526..1926)
                     /locus_tag="VFX87_11910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEX4995674.1"
                     /translation="MSALSIRTRLTLWYSGLLLVILIAIGALSYSWLRRTVHQDLDAS
                     LLAVARAIRETGVPDRPDGEVEALLRDMLGPELYDKFFRMLDPEGRPHAFGSPRRGDN
                     LPLSAAARAAAARGESTLETVAGPGAEPLRVFTMPIVRNGRVVDIVQVGTSVRRSEAL
                     LDRYLHTLIVLIPLGVGLAALGGAVFARAALRPVDEMTRTARRITAEDLSRRVERPGT
                     GDEMERLALTLNGMLSRLEEAFAQTRRFAADAAHELRTPLAALRGGIEVALRAERSPE
                     EYRRVLASSLEEVERLIRLAEDLLLLSRSTAGPEGPRAPVDLEPLLLDVFDVGARLGQ
                     AAGVSVRIDSMVPSTVCGDAAALRRALLNLVENAIKYTPPGGKVELSLTTAGGMAGLT
                     VSDAGIGIDSADAERIFEPFVRLDAARARDTGGAGLGLAIARSIAVAHGGTLSVESRP
                     GAGSRFLLRLPLAPAG"
     gene            complement(1923..>2470)
                     /locus_tag="VFX87_11915"
     CDS             complement(1923..>2470)
                     /locus_tag="VFX87_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4995675.1"
                     /translation="PFDLVVLDVMLPKRDGLDVLRTLRARRIQAPVLLLTARDEVADK
                     VAGLDAGADDYLAKPFAFEELLARVRALLRRGRGGAEPVLRVADLALDPATRAVTRGR
                     RRISLTAREYALLEYFLRNTDRVLTRPMITQHVWGMDFDPESNIVDVYVGYLRRKIDA
                     AGEPRLLQTVRGAGYTLSAEP"
BASE COUNT          351 a          881 c          925 g          313 t
ORIGIN      
        1 cggatgtcgg aggcgccctc caccacgtgg ttgttggtca cgatgtagcc ggccgggttg
       61 atgatgaagc ccgagcccat gctgcgcacg gaccggcgcg gcggctgctc ctgctcgaac
      121 tgcttgaaga atcgctggaa cggatcgttg tcgccgaacg gggtctggag ctggatgcgc
      181 tcttccacgc gcttggtgct gatgttgacg accgccggct tgagcgcccg ggtcagctcg
      241 acccagttcg gctgcggcgc caccgccgcg gtcgtggccg gcaccgcccg ctcgctccat
      301 agcggcgcgg gggtattcgt gtgaccgaga tgcggcgcga gcgcgacgag gcccacgacg
      361 gcggccgcca cggctcccat ccaggtggcg cgagttctgg tgacgaatcg gttcatggac
      421 atctccccct cctcgaggac tgacagtttg gcggactgac gaacggcctg ctcgaaccgt
      481 agcccgcccg gatgagagcc gggtgaggcg gagatgagaa ttcgcttatc cggccggggc
      541 caacgggagg cggaggagga aacggctgcc cgcgccgggc cggctctcca ccgagagggt
      601 gccgccgtgg gcaaccgcga tggagcgggc gatggccagg ccgagcccgg cgccgcccgt
      661 gtcacgggcg cgggccgcgt cgagccgcac gaagggctcg aagatccgct ccgcgtcggc
      721 cgagtcgatc ccgatgccgg cgtcggacac cgtgagcccc gccatgccgc ccgcggtggt
      781 caggctgagc tcgaccttgc cgcccggcgg cgtgtacttg atcgcattct ccaccaggtt
      841 gagcagggcc cggcgcagcg cggccgcgtc accgcacacg gtggacggca ccatcgagtc
      901 gatgcgcacg ctcaccccgg cggcctggcc gagccgagcg cccacgtcga agacgtcgag
      961 gagcaggggc tcgagatcga caggcgcgcg gggcccctcc ggccccgcgg tcgagcggga
     1021 gagcagcaac aggtcctcgg ccaggcggat caggcgctcc acctcctcga ggctcgaagc
     1081 gagcacccgc cggtactcct ccggcgagcg ctcggcccgc agggccacct cgatcccgcc
     1141 gcggagcgcg gccagcgggg tccgcagctc gtgggctgcg tcggccgcga accggcgcgt
     1201 ctgggcgaag gcctcctcga ggcgcgagag catgccgttc agggtcaggg ccagccgctc
     1261 catctcgtcc ccggtgccgg gccgctccac ccggcggctc aggtcctcgg ccgtgatacg
     1321 ccgggcggtg cgcgtcatct cgtccaccgg gcggagcgcg gcgcgcgcga agacggcccc
     1381 gccgagcgcg gcgaggccga cccccagcgg gatcagcacg atcagggtgt gcaggtagcg
     1441 gtcgagcagc gcctcggagc ggcgcaccga cgtccccacc tgcacgatgt ccaccacgcg
     1501 gccgttcctg acgatgggca tcgtgaacac ccggagcggc tccgcgccgg ggccggcgac
     1561 ggtctcgagc gtgctctccc cgcgcgcggc ggcggcccgg gccgccgcgg agaggggcaa
     1621 gttgtcgccg cgccgcgggc tcccgaacgc gtggggccgg ccctcggggt ccagcatgcg
     1681 gaagaacttg tcgtagagct cgggcccgag catgtcgcgg agcagcgctt cgacctcgcc
     1741 gtccggccgg tcgggcacgc cggtctcccg gatggcccgc gcgaccgcga gcagggaggc
     1801 gtcgaggtcc tggtgcacgg tgcggcgcag ccacgagtag gacagggcgc cgatcgcgat
     1861 caggatcacg agcaggagcc cgctgtacca gagggtgagc cgggtgcgga tggacagcgc
     1921 gctcacggct cggcgctcag cgtgtagccc gcgccgcgca ccgtctggag gagccggggc
     1981 tcgccggccg catcgatctt gcggcgcagg taccccacgt acacgtccac gatattgctc
     2041 tcggggtcga aatccatgcc ccagacgtgc tgggtgatca tcggccgggt gagcacccgg
     2101 tcggtgttgc gcaggaagta ctcgagcagc gcgtactccc gcgcggtgag cgagatgcgg
     2161 cgccggcccc gcgtgaccgc ccgcgtggcc ggatccaggg cgagatccgc cacccggagc
     2221 accggctcgg cgcccccgcg gccgcgccgc agcagcgccc gcacccgcgc gagcagctcc
     2281 tcgaaggcga agggcttggc caggtagtcg tcggcgcctg catcgagccc ggcgaccttg
     2341 tcggccacct cgtcccgagc ggtcaggagg agaaccgggg cctggatccg gcgcgcccgg
     2401 agggtccgga gcacgtcgag cccatcccgc ttgggcagca tcacgtcgag gacgacgagg
     2461 tcgaacggcg
//
LOCUS       DASUFA010000323        15263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1326143, whole genome shotgun sequence.
ACCESSION   DASUFA010000323 DASUFA010000000
VERSION     DASUFA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326143"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VFX87_11920"
     CDS             <1..129
                     /locus_tag="VFX87_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995676.1"
                     /translation="SGDLLAPILLSIPLQLLAYHIAVRLGRDVDQPRNLAKSVTVE"
     gene            259..2439
                     /locus_tag="VFX87_11925"
     CDS             259..2439
                     /locus_tag="VFX87_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX4995677.1"
                     /translation="MSYPAVTADSLLAPLNPPQREAVTTTKGPVLVLAGAGSGKTRVI
                     AHRIAYLLAVEGVDPRHVLAVTFTNKAAAEMRHRVEDLLLPAGIRPPLIATFHSACVR
                     ILRQRAAQAGLPPSFVIYDEDDRQSLVKEALRELDFDERTLTPSTVVHRISHCKNQML
                     TPADVEQAARTPREERLASLYRLYEEKLAAAGALDFDDLLLRVVRLLETVPEALRWYR
                     TVWSHVLVDEYQDTNRAQYRIVQLLTEEHRNLCVVGDPDQSVYRWRGADLRNILDFEK
                     DYPDCRVVALEQNYRSTKRILDIASAVIVNNTARKDKRLWTENGEGERAEVYRAWDEN
                     EESAFVAQKIRAAHREGTAFREIAVFYRTNAQSRVLEDALRRAGIPYLIVGGVRFYER
                     REIRDMVAYLRLISNPLDDVAFRRAVAAPARGIGKATLDRLADAARAAGVPLLMACAA
                     LPGDITGKQRRALEEFARLIARLGERRAGFTVPALIDEVCAASGYRDALKAERTAEAE
                     ARLENLEELVAASEELIASQLTAGVETVTLEAFLDSIALVADTDELDAEAGGVTLMTL
                     HSAKGLEFPVVFLTGLEEGVFPHSRSMQEPEELEEERRLCYVGITRAQERLMLSYALH
                     RRIQGYGVAEPSRFLLEIPEDLLHLINARRPEPVQVPVSGVSEPAPDEDFPFRLGAKL
                     RHARWGEGLLVGIQREGADVIATVHFASVGRKRLSLQYAHLEEI"
     gene            2442..2747
                     /gene="gatC"
                     /locus_tag="VFX87_11930"
     CDS             2442..2747
                     /gene="gatC"
                     /locus_tag="VFX87_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX4995678.1"
                     /translation="MAPSGPKISAKDVEHVARLARLDLSAAEKERMGRELDGILTYID
                     KLRALDTTGVEPTSHAVPVTNVMRDDVPSPSLPQADALGNAPDRQGDLFRVPKIIEE"
     gene            2755..4209
                     /gene="gatA"
                     /locus_tag="VFX87_11935"
     CDS             2755..4209
                     /gene="gatA"
                     /locus_tag="VFX87_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX4995679.1"
                     /translation="MLASLTAHELAARYRSGDATPTQATTEYLARIERIDPQLRAFLT
                     VTREDALRRAAESDARFKAGAPRGPLDGIPVALKDLFCTRGVRTTSGSKILDRFVPPY
                     DATVVARLDAAGAILLGKLNMDEFAMGSSTEHSAYGATRNPWDLARVPGGSSGGASAV
                     VAADLAALSLGTDTGGSIRQPAAFCGVLGMKPTYGRVSRYGVIAFASSLDQVGPFAKD
                     VEDAAVLLGTIAGADPMDSTAVDVPVPDYRAALGRGIEGMKIGVPAEYFIDGMDPEVE
                     RAVREAIAKLEKLGARAEPVSLPHSEYGLAAYYVIAPAEASSNLARYDGVKYGLRAPG
                     ARDLIDMYSKTRAAGFGNEVKRRLMLGTYVLSAGYYDAYYGQAQKVRTLVRRDFDQAF
                     ARVDLIVAPTTPGVAFKMGEKEDPLQMYLNDIFTIPVNLAGLPGISIPGGFTRTGLPI
                     GLQLIGRAFDEATLLRAAHAYQEETSWHKRKPSL"
     gene            4206..5666
                     /gene="gatB"
                     /locus_tag="VFX87_11940"
     CDS             4206..5666
                     /gene="gatB"
                     /locus_tag="VFX87_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX4995680.1"
                     /translation="MSETPRYETVIGLEVHAQLSTTTKMFCGCRATFGAAPNTQTCPV
                     CLGLPGSLPVINRRAIEFGAMTALALGCRVNGLNRFARKHYYYPDMPKNYQISQYEEP
                     LAEHGRLAVDAGGGPREIGIQRVHLEEDVGKLVHEGALETAPSSSVDFNRAGVPLMEI
                     VSKPDLQSPEEAAAYLKTLRAILIYLRVCDGNMEEGSLRCDANISLRPRGGAEYGTKV
                     EIKNMNSFRNVRDALEYEIRRQARALETGERIVQETRLWDPDRAITVSMRSKEFAHDY
                     RYFPEPDLPPLDLRAGWVDEVRAQLPELPAARRVRFQSAYVLSPYEADLLTQGRGLAD
                     YFEEAARGAGKPKAVANWVLNELLRELPADDDRAVAACPIPPANLVGLLALMDDGTIS
                     GKIAKDVFEKMYRSGDTAQAIVSREGLTQVADEGALGAAVDRVLGEHAKVVDDFRAGK
                     KAALGFLVGQVMKSTQGKANPAVVNRLLEEKIRKVQ"
     gene            5690..6361
                     /locus_tag="VFX87_11945"
     CDS             5690..6361
                     /locus_tag="VFX87_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4995681.1"
                     /translation="MIRLQHVSMVFGAGPSRVAAINDVSFHVAKGEFVVLSGASGAGK
                     TTLLRLLYAAETPSEGDIDVAGFDVAALRRAEIPRLRRSIGIVFQDSKLLAGRTVFEN
                     VAFVLRVLGTARRDITPKAFAALKAVGLSARAQAFPHQLSQGEAQRASLARAIVKSPG
                     LLLADEPTGNLDDEMANEILALVKDIWSRGTTVLFATHQARLVPQLKRRTLKLEGGRL
                     VKDEG"
     gene            6447..7331
                     /locus_tag="VFX87_11950"
     CDS             6447..7331
                     /locus_tag="VFX87_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4995682.1"
                     /translation="MFSFVMNEAMRDLRRAGRIGLAAILLIALSLTALGGFWLLSLNL
                     GRAIDQWGDRVRVIVYLKDEPVAAKLDDLLQRIEAVGGIQRLRYVSKAEALRALKRSL
                     GSQADVAEQLPRNPLPASVEVTPDAAIATPEGTRALVGRLVTLPDVEEVQGGSQWVDG
                     LAQFRRLFQGVGLVIGAVLAVAAILTVTTATTLVLHLRRDEMEIMRLVGATENVIRAP
                     RLLQGMAQGLVAAIMALAALEIAYALAVPRLEPLLPMTLGLERVTFLSPLQALALIAA
                     GTMLGALGGLLARGRAQV"
     gene            7328..8560
                     /locus_tag="VFX87_11955"
     CDS             7328..8560
                     /locus_tag="VFX87_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX4995683.1"
                     /translation="MRRAWRLGLIPALVSALLAPVALAQAPKRDTAEIGDKQQDLQQA
                     QKRLLEERQKAAAARKREAGVLSELEGIDRRLSDKRQQVAALDGRLKRAQADIGELQG
                     EIGRLLSRRSGQEEVLGRRLRALYKLQAQGGVLPIVLSGADPVEQAVQLRHLTTLASV
                     DARLIREYRVTSEVLADRKSRVEARGREMASLRSEADQERAEFDQEAAKRRALLAKVQ
                     SERAYHDRMVGELSEATRRLEAFIRDLQEKQRRAVAKVPPPSRPARPAPGDASPAPGF
                     AALRGRLSWPADGRVVAEYGPQVNPRFGTKTFRNGIDIEAHEGSNIATVFPGQVVYTG
                     WFRGYGNLIIVDHGGEYYTVYAHAADIRVTEGDEVKQGQIIGTVGDTGSLQGPRLYFE
                     VRHEGKPQDPTQWLRPRG"
     gene            8627..9910
                     /locus_tag="VFX87_11960"
     CDS             8627..9910
                     /locus_tag="VFX87_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4995684.1"
                     /translation="MRPIRHMKKVVMISALLLILTLSLGGGVASKGNDTAATYENLRL
                     FTEVLSIVQSQYVDEVAPKDIIYNAIKGTLRGLDPHSSFLDPEMYREMQVETSGSFGG
                     LGIEITLRDDVLTVVAPIEGTPAYRAGIQPGDRILKIEGLTTKDMQLSDAVKRMRGKP
                     GSKITISVVREGWTEPKDFLITREQIRVHSVRANQLEPGIEYIKLRQFQEQTANDLET
                     SLEKYVKDGKIQGLVLDLRNNPGGLLTSAVEVTEKFLESGKLVVYTEGRVRNQNMRFS
                     SNAKRVFTDFPIVVLVNQGSASASEIVAGALQDWGRAVVIGTQSFGKGSVQTIIPLSD
                     GSGLRLTTAKYFTPKGRSIHGKGITPDIIVEAPKVVASAPGAEVPVPTPPVSETSQEQ
                     LKRDPQLQRALDLLKAMKILDKGRPGPAGPQAQTR"
     gene            9928..10800
                     /locus_tag="VFX87_11965"
     CDS             9928..10800
                     /locus_tag="VFX87_11965"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX4995685.1"
                     /translation="MPARVWQALAGIIILLLIAVVGLDQWQARSGEASLFGSDLFGRA
                     ARRPAAPAAPATLSRALPEAAPLPPGAPRVAVIVDDLGSRRDVFDVLREIRRPLTVAV
                     LPALPLSAAIARDASRAGMEVLLGLPMEPYRFPEVDPGPGALLMSMAPGEIQHLVGSH
                     LEAVAPAVGVVNRMGSRLTEDRARMRAVLEVLAARRLFLVDAYTSSQSVAFDEARDAG
                     VRAARRQILVDHARGEAGDRARWDEVAGWAERRGEVIVVAHGHPLTVRLLKEYVPRWE
                     ARGLRLVPVSQLAR"
     gene            10811..11812
                     /gene="tsaD"
                     /locus_tag="VFX87_11970"
     CDS             10811..11812
                     /gene="tsaD"
                     /locus_tag="VFX87_11970"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX4995686.1"
                     /translation="MIVLAIETSCDETAAAVVDGGRKILSSVVASQDDVHAPYGGVVP
                     ELASRRHLEVLMPVVRRALETAAIRLDDVDGLAVTYGPGLVGSLLIGCSAAKAIAYAR
                     RRPLVGVNHLEGHIYAAFLEGDPPAFPFLALVVSGGHTALYLVREARRYERIGQTRDD
                     AAGEAFDKVAKLLGLGFPGGPAIERVARDGDPRAIVFPTANMSDGASDFSFSGIKTAV
                     SLHVRRAGALGPAQVADIAASFQATAVKMLVRKTVKAALRAGVRRLVLTGGVAANTAL
                     REALEGECRERGWALHVPSRRLCTDNAAMIAAAGHDRLEVGERAPLSLNAVPDLVLA"
     gene            11809..12894
                     /locus_tag="VFX87_11975"
     CDS             11809..12894
                     /locus_tag="VFX87_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4995687.1"
                     /translation="MSPRLDYEALIAGLPDAVVGVDEARRVMLWNPAAEALLGRSARR
                     TIGRALKEVFPPDTSLVRHLTDTLSTGESRSESEAVIEGGDGRPVHVSVVTAPLAVRS
                     GGVEAAVAVIRDMTRLQQLESEVRRGETLAAAGQIAIGLAHEIRNPLGAIRGAVQLMA
                     REMGDDARLGEYTSVLLKEVDRVNRILEMLLDISRPVTLRPVPLNVHQLLERVALLSE
                     ELAARRGVQIVRRYDPSLPPILADEDRILQVFHNLVRNAVEAMTAGGRLTLVTRLSMN
                     PLFAKVDLGHGQRSMAEIQVLDEGQGIPAATLSRLFTPFFTTKDKGLGLGLALCHRII
                     DEHRGAIQIGSEPGRGTAVSCFLPIAR"
     gene            12907..14334
                     /locus_tag="VFX87_11980"
     CDS             12907..14334
                     /locus_tag="VFX87_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4995688.1"
                     /translation="MPDGRILIADDEDSLRWVLEKGFRGAGYQVTAVKDGTAALREVE
                     GGPFDLILLDIRMPGIDGLSLLKQVRANRPDAQVVIMTAHGTMETAVAAMQDGAYDYL
                     AKPFDLDEALLLAERALTARRLTQEVTALRSGLKEVWEFGALVGRHPTMQEVYKVIGR
                     VAASDVSVLLRGESGTGKEVVARALHHYSRRAGRPFMGISAAAIPINLLESELFGHEK
                     GSFTDAKERRLGKLELAHGGSVFFDEIGDMPAELQVKLLRALQERSFERVGGHELIRM
                     DVRVLAATHRDLEAMMKSGQFREDLFYRLNVVTLSLPALRDRRGDIPLLAEHFLAKYA
                     ESLGERVVASDAMDRLVGHDWPGNVRELENVIQRAMVMASSGVILPEHLPIGPVSAAA
                     AVGTDASLEDVIERKMHECVRGLRGHASANLHGLMVGLVEKPLLRAVMRETKGNQVRA
                     AQLLGINRNTLRKKLKEHGIDPDAV"
     gene            14341..14916
                     /locus_tag="VFX87_11985"
     CDS             14341..14916
                     /locus_tag="VFX87_11985"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX4995689.1"
                     /translation="MPTRCGWANGGPLEIAYHDAEWGVPSHDDRHLFEMLVLEGAQAG
                     LSWSTILRKRENYRKAFAGFDPVKVARFDARRRAALMRDAGIVRNRQKIEATVGNARQ
                     VLAVQEEFGSLAEYLWQFVGGKPIQNAWAAGARVPAETAESRALSRALVKRGFRFIGP
                     TICYAFMQATGMVNDHITTCFRYPRRRSQRS"
     gene            14913..>15263
                     /locus_tag="VFX87_11990"
     CDS             14913..>15263
                     /locus_tag="VFX87_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4995690.1"
                     /translation="MRIGIIGAGAIGCVVGGLLTKAGHDVTLVDQWPEHIETMKQRGL
                     RLSGTCGEHTIPVKALHIHELQGVATPFEAVFVSVKSYDTEWAAQMALAYLKQPDGVV
                     VDFQNGVNDERVASV"
BASE COUNT         2293 a         5355 c         5464 g         2151 t
ORIGIN      
        1 tcgggcgatc tgctcgcgcc catcctgctg tcgatcccgc tgcagctcct ggcctaccac
       61 atcgcggtcc gcctcggccg cgacgtcgat cagccccgga acctcgccaa gtccgtcacc
      121 gtcgagtaga gcgaggccgc agccgaaggc gcgccgagcg gatacaagag ggaaggtccc
      181 gccgtacggg cgctccccac cggggagagg gcagggtgag gggcacgacc cctcgcggtt
      241 gtgttatcgt ttgttcccat gtcgtaccct gcggtgaccg cggacagtct cctcgcgccc
      301 ctgaacccgc cgcagcgtga ggcggtcacg accacgaagg gccccgtgct cgtgctggcc
      361 ggggcgggga gcggcaagac ccgggtcatc gcccaccgca tcgcctatct cctggcggtg
      421 gagggcgtcg acccgcggca cgtgctcgcg gtcaccttca ccaacaaggc cgccgccgag
      481 atgcggcatc gcgtcgagga cctgctgctg cccgcgggga tccgcccgcc gctcatcgcc
      541 accttccact cggcgtgcgt gcgcatcctg cggcagcgcg cggcccaggc cgggctcccg
      601 ccctccttcg tgatctacga cgaggacgac cggcagagcc tggtgaagga ggccctgcgc
      661 gagctcgact tcgacgagcg cacgctcacg cccagcacgg tggtgcaccg catcagccac
      721 tgcaagaacc agatgctcac gcccgccgac gtggagcagg ccgcccgcac cccgcgcgag
      781 gagcgcctcg cctccctcta ccggctctac gaggagaagc tcgccgccgc gggcgcgctc
      841 gacttcgacg acctgctgct tcgggtggtg cggctcctcg agacggtccc ggaggcgctg
      901 cgctggtacc gcaccgtatg gagccacgtc ctggtggacg agtaccagga caccaaccgc
      961 gcgcagtacc gcatcgtgca gctcctcacc gaggagcacc ggaacctctg cgtggtgggc
     1021 gaccccgacc agtcggtcta tcgctggcgc ggcgcggacc tgcgcaacat cctcgacttc
     1081 gagaaggact acccggactg ccgggtggtg gcgctggagc agaactaccg ctccaccaag
     1141 cgcatcctcg acatcgcctc cgcggtgatc gtcaacaaca ccgcccgcaa ggacaagcgg
     1201 ctctggaccg agaacgggga aggcgagcgc gcggaggtct accgggcctg ggacgagaac
     1261 gaggagtcgg ccttcgtggc ccagaagatc cgggccgccc accgtgaggg gaccgcattt
     1321 cgagagatcg cggtgttcta ccgcaccaac gcccagtccc gcgtcctgga ggacgcgctg
     1381 cgccgcgcgg gcatcccgta cctgatcgtg ggcggcgtgc gcttctacga gcggcgcgag
     1441 atccgcgaca tggtggccta cctgcgcctg atctcgaacc cgctcgacga cgtggccttc
     1501 cgccgggcgg tcgccgcccc cgcccgcggc atcggcaagg ccacgctcga tcgcctggcc
     1561 gacgcggcgc gcgcggcggg cgtgccgctc ctgatggcct gcgcggccct gccgggcgac
     1621 atcaccggca agcagcggcg cgccctcgag gagttcgccc gcctcatcgc ccgcctcggg
     1681 gagcggcgtg ccggcttcac ggtgcccgcg ctgatcgacg aggtgtgcgc ggcctccggc
     1741 taccgcgacg cgctcaaggc ggagcgcacc gccgaggcgg aggcgcgcct cgagaacctg
     1801 gaggagctgg tggccgcctc cgaggagctc atcgcgagcc agctcacggc gggcgtcgag
     1861 acggtgaccc tcgaagcctt cctcgactcg atcgccctgg tggccgacac cgacgagctc
     1921 gacgcggagg cgggcggggt gaccctcatg accctgcact cggccaaggg gctcgagttc
     1981 ccggtggtct tcctcaccgg cctcgaggaa ggggtcttcc cgcactcacg gtccatgcag
     2041 gagccggagg agctggagga ggagcggcgc ctctgctacg tcggcatcac gcgcgcccag
     2101 gagcggctga tgctgtcgta cgccctgcac cgccgcatcc agggctacgg ggtcgcggag
     2161 ccgtcgcgct tcctgctcga gatcccggaa gacctgctcc acctgatcaa tgcgcggcgg
     2221 cccgagccgg tccaggtgcc cgtctccggc gtctcggagc ccgcgcccga cgaggacttc
     2281 ccgtttcgcc tgggggccaa gctgcgccac gcgcggtggg gcgaagggct gctggtgggc
     2341 atccagcgcg agggcgccga cgtgatcgcc accgtgcact tcgcgagcgt gggccgcaag
     2401 cgcctgtcgc tgcagtacgc gcatctcgag gagatctagc gatggccccc tccggcccga
     2461 agatctcggc gaaggacgtc gagcacgtgg cccggctggc ccgtctcgac ctgtcggcgg
     2521 ccgagaagga gcggatgggc cgcgagctcg acggcatcct gacctacatc gacaagctcc
     2581 gcgcgctcga caccaccggc gtcgagccca cctcgcacgc ggtgcccgtc accaacgtga
     2641 tgcgggacga cgtgccgtcc ccgtcgctgc cccaggccga cgcgctcggc aatgcgccgg
     2701 accggcaggg cgatctcttc cgcgtcccca agatcatcga ggagtagcgg ccgcatgctg
     2761 gcctcgctca ccgcgcacga gctcgcggcc cggtaccgga gcggtgacgc cacgcccacg
     2821 caggccacga ccgagtacct ggcccggatc gagcgcatcg atccgcagct ccgggccttt
     2881 ctcaccgtca cccgggagga cgcgctccgc cgcgcggccg aatccgacgc ccgcttcaag
     2941 gcgggcgccc cgcggggacc gctcgacggc atcccggtcg ccctcaagga cctcttctgc
     3001 acgcgcggcg tccgcaccac ctcggggtcg aagatcctcg accgcttcgt gccgccgtac
     3061 gacgcgacgg tggtcgcgcg gctcgacgcg gcgggggcga tcctgctcgg caagctcaac
     3121 atggacgagt tcgcgatggg ctcgtccacc gagcactccg cctacggcgc cacccgcaac
     3181 ccgtgggacc tggcgcgggt gcccggcggc tcctcgggcg gcgcctccgc ggtggtggcc
     3241 gcggatctcg ccgcgctcag cctcggcacg gataccggcg gctcgatccg ccagcccgcc
     3301 gccttctgcg gcgtgctcgg catgaagccc acctacgggc gcgtgtcgcg ctacggggtc
     3361 atcgcgttcg cgtcctcgct ggaccaggtc ggccccttcg ccaaggacgt ggaggacgcc
     3421 gcggtgctgc tcgggaccat cgcgggcgcc gatccgatgg actcgaccgc ggtggacgtg
     3481 ccggtccccg actaccgggc cgcgctcggg cgggggatcg aggggatgaa gatcggggtg
     3541 cccgcggagt acttcatcga cgggatggat cccgaggtgg agcgggcggt gcgcgaggcc
     3601 atcgccaagc tcgagaagct gggcgcccgc gcggagccgg tctcgctccc gcactcagag
     3661 tacgggctcg ccgcctacta tgtcatcgcg cccgcggagg cgtcgtcgaa cctcgcgcgc
     3721 tacgacgggg tgaagtacgg cctgcgggcg cccggcgcgc gcgacctgat cgacatgtac
     3781 agcaagaccc gggccgccgg attcggcaac gaggtcaagc gccggctgat gctggggacc
     3841 tacgtgctct ccgccggcta ctacgacgcc tattacggcc aggcccagaa ggtccggacg
     3901 ctggtgcggc gcgacttcga ccaggccttc gcccgcgtgg acctgatcgt ggcccccacc
     3961 acgcccggcg tggcgttcaa gatgggcgag aaggaggatc cgctgcagat gtacctgaac
     4021 gacatcttca cgattccggt gaatctcgcg gggctccccg gcatctcgat cccgggcggc
     4081 ttcacccgga ccggccttcc gatcggcctg cagctcatcg gccgggcgtt cgacgaggcg
     4141 acgctgctgc gcgcggcgca cgcctatcaa gaagaaacat cgtggcacaa gcgaaagccc
     4201 tcgctatgag cgagaccccg cgctacgaaa cagtcatcgg tctcgaggtt cacgcccagc
     4261 tctccaccac gaccaagatg ttctgcggtt gccgggccac cttcggggcg gcaccgaaca
     4321 cccagacctg tccggtctgc ctgggattgc ccggctcact cccggtgatc aaccggcgcg
     4381 ccatcgagtt cggcgcgatg accgccctgg cgctcggctg ccgggtgaac ggcctgaacc
     4441 gcttcgcgcg caagcactac tactaccccg acatgccgaa gaactaccag atcagccagt
     4501 acgaggagcc gctcgccgag cacgggcgcc tcgcggtgga cgcgggcggc gggccgcgcg
     4561 agatcgggat ccagcgcgtg cacctcgagg aggacgtggg caagctcgtc cacgagggcg
     4621 cgctcgagac cgcgccgtcg agcagcgtgg acttcaaccg ggccggggtg cccttgatgg
     4681 agatcgtctc caagcccgac ctccagagcc cggaggaggc cgcggcctac ctgaagacgc
     4741 tccgggcgat cctgatctac ctgcgcgtgt gcgacggcaa catggaggag ggctcgctcc
     4801 gctgcgacgc caacatctcg ctccggcccc gcggcggggc cgagtacggc accaaggtcg
     4861 agatcaagaa catgaactcg ttccggaacg tgcgcgacgc gctcgagtac gagatccggc
     4921 ggcaggcgcg cgcgctcgag accggggagc gcatcgtcca ggagacgcgg ctctgggatc
     4981 ccgatcgggc catcaccgtc tcgatgcgct cgaaggagtt cgcgcacgac taccgctatt
     5041 tccccgagcc cgacctgccg ccgctcgatc tgcgcgcggg ctgggtggac gaggtgcgcg
     5101 cgcagctccc cgagctgccg gccgcccgcc gggtccgttt ccagagcgcg tacgtgctgt
     5161 ccccctacga ggccgatctg ctgacccagg gccgcggact cgcggactac ttcgaggagg
     5221 ccgcccgcgg cgccggcaag cccaaggcgg tggccaactg ggtgctcaac gagctcctgc
     5281 gcgagctgcc cgcggacgac gaccgcgcgg tggccgcctg cccgatcccg cccgccaacc
     5341 tggtcgggct gctggccctg atggacgacg gcaccatcag cggcaagatc gccaaggacg
     5401 tgttcgagaa gatgtaccgc tcgggcgaca cggcgcaggc gatcgtgagt cgcgaggggc
     5461 tcacccaggt ggccgacgag ggcgcgctgg gcgccgcggt ggaccgggtc ctgggcgagc
     5521 acgccaaggt ggtggacgat tttcgggccg gaaagaaggc cgcgctgggc tttctggtcg
     5581 gccaggtgat gaaatccacc cagggcaagg ccaatccggc ggtggtgaac cgattgttgg
     5641 aggaaaaaat acgaaaggtc caatagttag atagaatagt ttgcacctca tgattcggct
     5701 gcagcacgtg tcgatggtct tcggtgccgg cccctcccgt gtggccgcca tcaacgacgt
     5761 gtccttccat gtcgccaagg gggagttcgt cgtcctgagc ggggccagcg gcgcgggcaa
     5821 gactaccctg ctccggctcc tctacgcggc cgaaacgccc tccgagggcg acattgacgt
     5881 ggccggcttc gacgtcgcgg ctctccgccg cgccgagatc ccgcgcctgc ggcggtccat
     5941 cggtatcgtc ttccaggact ccaagctgct ggccggccgc accgtattcg agaacgtggc
     6001 cttcgtgctc cgcgtgctcg ggacggcccg ccgtgacatc acgccgaagg ccttcgccgc
     6061 gctcaaggcg gtcgggctct ccgcgcgggc ccaggccttc ccgcaccagc tctcccaggg
     6121 ggaagcgcag cgcgcctcgc tggcgcgggc catcgtcaag tcgccggggc tgctcctggc
     6181 cgacgagccc accggcaacc tggacgacga gatggccaac gagatactgg cgctggtcaa
     6241 ggacatctgg agccgcggga ccaccgtgct cttcgcgacg caccaggccc gcctcgtccc
     6301 ccagctcaaa cgccgcacct tgaagctgga aggcgggcgc ctcgtgaagg acgaaggatg
     6361 agccgggcgc tccgggtcga cccgaccgag atggcccacg gtcaacatgg cttagccatg
     6421 ttgaggatga agaggggtta gggttcttgt tcagcttcgt catgaacgag gcgatgcgcg
     6481 acctgcggcg cgccggccgc atcggcctgg cggccatcct gctcatcgcg ctctcgctca
     6541 ccgccctcgg cggcttctgg ctgctctcgc tcaacctggg ccgggccatc gatcagtggg
     6601 gcgaccgggt gcgcgtcatc gtgtacctga aggacgagcc ggtggccgcc aagctcgacg
     6661 atctcctgca gcgcatcgag gcggtggggg gcatccagcg gctgcgctac gtctccaagg
     6721 ccgaggccct gcgcgcgctg aaacgctcgc tgggcagcca ggccgacgtg gccgagcagc
     6781 tgccgcgcaa tccgctcccc gcctcggtcg aggtgacgcc cgacgcggcc atcgcgacgc
     6841 cggagggcac ccgggccctg gtgggccgtc tggtgacgct gccggacgtg gaggaagtgc
     6901 agggcggcag ccagtgggtg gacgggctcg cccagttccg ccgcctgttc cagggggtcg
     6961 gtctcgtgat cggcgcggtc ctggcggtgg ccgcaatcct caccgtgacc accgcgacga
     7021 ccctggtcct gcacctccgc agagacgaga tggagatcat gcggctggtg ggcgccaccg
     7081 agaacgtgat ccgcgcgccg cggctgctcc agggtatggc ccagggcctg gtcgccgcca
     7141 tcatggcgct cgccgcgctc gagatcgcct acgcgctcgc ggtgccccgc ctcgagcccc
     7201 tgctgccgat gacgctcggc ctcgagcggg tgaccttcct ctctccgctg caggcgctcg
     7261 ccctcatcgc cgccgggacc atgctgggag cgctgggcgg gctgctggcc cgcgggcggg
     7321 cccaggtatg aggcgcgcct ggcggctcgg cctgatcccg gcgctcgtgt cggccctcct
     7381 cgcgccggtg gccctggccc aggccccgaa gcgcgacacg gccgagatcg gcgacaagca
     7441 gcaggacctc cagcaggcgc agaagcgcct cttggaggag cgtcagaagg cggccgccgc
     7501 gcgcaagcgc gaggcgggcg tgctgagcga gctcgagggc atcgatcggc ggctcagcga
     7561 caagcgccag caggtcgcgg ccctggacgg gcgcctcaag cgggcgcagg ccgacatcgg
     7621 cgagctgcag ggcgagatcg ggcggctgct gtcgaggcgc tccgggcagg aggaggtgct
     7681 gggccggcgc ctgcgcgcgc tctacaagct gcaggcccag ggcggcgtgc tgccgatcgt
     7741 gctctcgggc gcggacccgg tggagcaggc ggtccagctc cggcacctga cgaccctggc
     7801 ctcggtggac gcccggctga ttcgagagta tcgtgtgacg tccgaagtgc tggcggaccg
     7861 gaagagccgg gtcgaggcgc gcgggcggga gatggcgtcg ctccgatccg aggcggatca
     7921 ggagcgggcc gagttcgatc aggaggcggc gaagcggcgc gcgctcctcg cgaaggtgca
     7981 gagcgagcgc gcctaccacg accggatggt cggcgagctg tccgaggcca ctcgccggct
     8041 cgaggccttc atccgggatc tgcaggagaa gcagcggcgg gcggtggcca aggtgccgcc
     8101 gccgagccgg cccgcgcggc cggcgccggg cgacgcgagc ccggctccgg gcttcgcggc
     8161 cctgcgcggg cggctgtcct ggccggcgga cggccgggtc gtcgccgagt acggcccgca
     8221 ggtcaatccg cgcttcggaa cgaagacgtt ccgcaacggg atcgacatcg aggcccatga
     8281 gggctcgaac atcgcgacgg tgttccccgg ccaggtcgtc tacaccggct ggttccgggg
     8341 ctacggcaac ctgatcatcg tggatcacgg cggcgagtac tacacggtgt acgcgcacgc
     8401 cgccgacatc cgggtcaccg agggcgacga ggtgaagcag gggcaaatca tcggcacggt
     8461 cggcgatacc ggctcgctgc aggggccgcg cctgtatttt gaagtgcggc acgaagggaa
     8521 gccgcaggat cccacgcagt ggctcaggcc acgcggctag gtggcttggc gaggcgcagc
     8581 cgaaggcgag ccgagcgaaa atgaaagtac ccaggaggag tacacgatgc ggcccatccg
     8641 gcacatgaag aaggtggtca tgatctccgc gctgctgctc atcctcaccc tgtccctcgg
     8701 tggcggggtg gcgagcaagg gcaacgacac cgcggccacc tacgagaacc tccgcctctt
     8761 caccgaggtg ctctcgatcg tgcagagcca gtacgtggac gaggtcgcgc ccaaggacat
     8821 catctacaac gcgatcaagg ggactcttcg agggctggac ccccactcat cgttcctcga
     8881 ccccgagatg taccgggaga tgcaggtcga gacgagcggc agcttcgggg ggctcggcat
     8941 cgagatcacg ctgcgcgacg acgtgctcac cgtggtggcc cccatcgagg gcaccccggc
     9001 ctatcgcgcg gggatccagc ccggcgaccg catcctgaag atcgaggggc tcaccaccaa
     9061 ggacatgcag ctctccgacg cggtcaagcg catgcggggc aagccgggca gcaagatcac
     9121 gatctcggtc gtgcgcgagg gctggacgga gccgaaggac ttcctcatca cccgcgagca
     9181 gatccgcgtg cattcggtcc gggccaacca gctcgagccg ggcatcgagt acatcaagct
     9241 gcgccagttc caggagcaga ccgccaacga cctggagacc tccctcgaga agtacgtgaa
     9301 ggacggaaag atccaggggc tggtgctgga cctgcgcaac aatccgggtg gcctcctcac
     9361 ctccgcggtg gaggtgaccg agaaattcct ggagtccggc aagctggtgg tctacaccga
     9421 ggggcgcgtg cgcaaccaga acatgcgctt ctcgtcgaac gccaagcgcg tgttcaccga
     9481 cttcccgatc gttgtgctgg tcaaccaggg cagcgcctcc gcctccgaga tcgtggcggg
     9541 cgcgctccag gactggggcc gcgcggtggt gatcggcacc cagagcttcg gcaagggctc
     9601 ggtgcagacc atcatcccgc tctcggatgg ctcgggcctg cggctgacca ccgcgaagta
     9661 cttcacgccg aagggccgct cgatccacgg caagggcatc acccccgaca tcatcgtgga
     9721 ggcgccgaag gtggtggcgt ccgcgcccgg tgccgaggtg cccgtgccga ccccgccggt
     9781 gtcggagacg tcgcaggagc agctcaagcg ggatccgcag ctgcagcgcg cgctcgacct
     9841 gctgaaggcc atgaagatcc tggacaaggg ccggccggga ccggccgggc cccaggccca
     9901 gacccgctag gcggccggag gggacctatg ccggcgagag tgtggcaggc tctcgccggc
     9961 atcatcattt tgctgctgat cgcggtggtc gggctggatc agtggcaggc ccggagcggc
    10021 gaggcgagcc tgttcggctc cgatctcttc gggcgcgcag cccggaggcc cgcggcgccg
    10081 gccgctcccg cgacgctctc ccgagcgctt cccgaggcgg cgccgctgcc cccgggcgct
    10141 ccccgggtgg ccgtcatcgt ggacgacctc ggcagccggc gcgacgtgtt cgacgtcctg
    10201 cgcgagatcc gccgcccgct cacggtggcg gtgctgcccg cgctgccgct gtcggccgcc
    10261 atcgcgcggg acgcctcccg cgcgggtatg gaggtgctgc tggggctgcc gatggagccg
    10321 taccggttcc cggaggtgga tccggggccg ggcgcgctgc tgatgtcgat ggccccgggc
    10381 gagatccagc acctcgtggg cagtcatctg gaggcggtgg cgcccgcggt cggcgtggtg
    10441 aaccgcatgg gctcgcgact caccgaggat cgcgcgcgca tgcgggcggt gctggaagtg
    10501 ctcgccgcgc ggcgcctctt cctggtagac gcctacacgt cgagccagtc ggtggccttc
    10561 gacgaggcgc gggacgcagg cgtgcgcgcg gcccgccggc agatcctggt ggaccacgcg
    10621 cgcggggagg cgggagatcg ggcccgctgg gacgaggtgg cgggttgggc agagcggcgg
    10681 ggcgaggtga tcgtggtggc ccacggccat ccgctgacgg tgaggctctt gaaggaatac
    10741 gtgccccggt gggaagcgcg ggggctccgg ctggtaccgg tctcccaact cgcgcgataa
    10801 tcgagccgac atgatcgtcc tcgccatcga gacctcctgc gacgagacgg ccgccgccgt
    10861 cgtggacggg ggccgcaaga tcctctcgag cgtcgtggcc tcccaggacg acgtgcatgc
    10921 cccctacggc ggcgtggtgc cggagctggc gtcgcgccgg cacctcgagg tgctgatgcc
    10981 ggtggtgcgc cgggcgctcg agacggccgc gatccggctc gacgatgtgg acggcctcgc
    11041 ggtcacctac gggcccggac tcgtgggctc gctgctcatc ggctgctcgg cggccaaggc
    11101 gatcgcctac gcgcgtcgcc gacccctggt cggggtcaac cacctcgagg gccacatcta
    11161 cgcggcgttc ctcgagggcg acccgcccgc cttcccgttc ctggccctgg tcgtctcggg
    11221 cgggcacacc gcgctctacc tggtgcggga ggcgcggcgc tacgagcgca tcggccagac
    11281 gcgcgacgac gcggcggggg aagccttcga caaggtggcg aagctgctcg gcctggggtt
    11341 cccgggcggg cccgccatcg agcgggtggc gcgggacggg gacccgcgcg cgatcgtgtt
    11401 ccccaccgcc aacatgagcg acggggcgtc cgacttctcg ttcagcggca tcaagaccgc
    11461 ggtctcgctc cacgtgcgcc gcgcgggcgc gctcggtccc gcgcaggtcg ccgacatcgc
    11521 cgcgtccttc caggccaccg cggtcaagat gctggtgcgc aagaccgtca aggccgcgct
    11581 gcgtgccgga gtgcggcggc tggtgctcac cggcggagtg gccgccaaca ccgcgctgcg
    11641 ggaggccctc gagggcgagt gccgcgagcg cgggtgggcg cttcacgtgc cctcgcgccg
    11701 gctctgcacc gacaacgcgg ccatgatcgc ggccgccggc cacgaccggc tggaggtggg
    11761 cgagcgcgcc ccgctgtcgc tcaatgccgt ccccgatctg gtgctggcat gagcccgcgt
    11821 ctcgactacg aagcgctcat cgccgggctg cccgacgcgg tggtgggcgt ggacgaagcc
    11881 cggcgcgtga tgctctggaa tccggccgcg gaggcgctcc tcggccggtc ggcccggcgg
    11941 accatcgggc gcgcgctcaa ggaagtcttc ccgccggata cctcgctggt gcggcatctg
    12001 accgacacgc tgtccaccgg ggagagccgg tcggagtcgg aggcggtgat cgagggtggg
    12061 gacggccgcc ccgtgcacgt gagcgtggtc accgcgccgc tggcggtgcg gagcgggggc
    12121 gtggaggccg cggtggcggt gatccgcgac atgacccggc tccagcagct cgagtcggag
    12181 gtgcggcgcg gcgagacgct ggcggcggcc gggcagatcg cgatcgggct ggcccacgag
    12241 atccgcaatc cgctcggggc catccggggg gccgtgcagc tcatggcccg cgagatgggt
    12301 gacgacgccc gcctcggcga gtacaccagc gtgctgctca aggaagtgga ccgcgtcaac
    12361 cggatcctcg agatgctgct cgacatcagc cgcccggtca ccctgcgccc ggttcccctc
    12421 aacgtgcacc agctcctcga gcgcgtggcg ctcctttccg aggagctggc ggcccggcgc
    12481 ggcgtgcaga tcgtccggcg ctacgacccg agcctgccgc cgatcctcgc cgacgaggac
    12541 cgcatcctgc aggtcttcca caacctcgtc cgcaacgcgg tggaggcgat gacggcgggc
    12601 ggccgcctga ccctggtcac gcggctcagc atgaacccgc tcttcgccaa ggtggacctg
    12661 gggcacggcc agcgcagcat ggccgagatc caggtgctgg acgagggtca gggcatcccg
    12721 gcggctaccc tctcccggct cttcaccccg ttcttcacca ccaaggacaa gggcctcggc
    12781 ctgggcctgg ccctctgcca ccggatcatc gacgagcatc gcggcgcgat ccagatcggc
    12841 agcgagcccg gccgcggtac cgcggtctcc tgctttctcc cgatcgcccg gtagaggatc
    12901 cgcgccatgc ccgacggacg catcctgatc gccgacgacg aggacagcct gcgctgggtc
    12961 ctcgagaagg gcttccgcgg cgcgggctat caggtcaccg cggtcaagga cggcaccgcg
    13021 gcgctccgcg aggtcgaggg cgggccgttc gacctgatcc tcctcgacat ccggatgccc
    13081 ggcatcgacg gcctctcgct gctcaagcag gtgcgcgcca accggccgga cgcgcaggtc
    13141 gtgatcatga ccgcgcacgg cacgatggag accgcggtgg ccgccatgca ggacggcgcc
    13201 tacgactacc tggccaagcc cttcgatctc gacgaggcgc tccttctggc ggagcgggcc
    13261 ctcaccgcgc ggcgcctcac ccaggaggtg accgcgctcc gctccggtct caaggaggtc
    13321 tgggagttcg gcgcgctggt gggccggcac ccgaccatgc aggaggtcta caaggtcatc
    13381 ggccgcgtgg cggccagcga cgtcagcgtg ctgctgcgcg gggagtccgg caccggcaag
    13441 gaggtggtgg cgcgcgcgct gcaccactac agccgccgcg cgggacggcc gttcatgggc
    13501 atctcggccg cggccattcc catcaacctg ctcgagtccg agctgttcgg ccacgagaag
    13561 ggctcgttca ccgacgccaa ggagcgccgc ctcggcaagc tcgagctggc ccacggtggc
    13621 tcggtgttct tcgacgagat cggcgacatg ccggccgagc tgcaggtgaa gctgctgcgc
    13681 gccctgcagg agcgcagctt cgagcgcgtc ggagggcacg agctgatccg catggacgtg
    13741 cgcgtgctcg cggccaccca ccgggacctc gaggccatga tgaagtcggg gcagttccgg
    13801 gaagacctgt tctaccgctt gaacgtcgtc acgctctcgc tgcccgccct ccgcgaccgc
    13861 cgcggcgaca tcccgctgct cgccgagcac ttcctggcca agtacgccga gtcgctgggc
    13921 gagcgggtgg tggcctccga cgccatggat cggctcgtcg gccacgattg gccgggcaac
    13981 gtgcgcgagc tggagaacgt catccagcgg gccatggtca tggcctcctc gggcgtgatc
    14041 ctgcccgagc acctgcccat cgggccggtg tcggcggcgg ccgcggtggg caccgacgcc
    14101 tcgctcgagg acgtcatcga gcggaagatg cacgagtgcg tgcgcggcct gcgcggccac
    14161 gcctccgcga acctccacgg cctcatggtc gggctggtgg agaagcccct gctgcgggcg
    14221 gtgatgcggg agaccaaggg taaccaggta cgcgcggctc agctcctcgg catcaaccgg
    14281 aataccctgc gcaagaagct caaggaacac ggcatcgacc ccgacgccgt ctaggagacg
    14341 atgccgaccc gttgcggctg ggccaatggc ggtccgctgg agatcgcgta ccacgacgcc
    14401 gagtggggcg tgccgtccca cgacgatcgg cacctcttcg agatgctcgt gctggagggc
    14461 gcccaggccg gtctctcctg gtccacgatt ctgcgcaagc gggagaacta ccggaaggcc
    14521 tttgcgggat tcgatcccgt caaggtcgcc cggttcgacg ctcgccggcg ggccgccctc
    14581 atgcgggacg cgggcatcgt gcgcaaccgg cagaagatcg aggcgacggt gggcaatgcc
    14641 cggcaggtgc tggcggtgca ggaggagttc ggcagcctcg cggagtacct gtggcagttc
    14701 gtcggcggca agccgatcca gaacgcgtgg gcggccggcg cgcgggtccc ggcggagacc
    14761 gcggagtcgc gtgcgttgag ccgcgcgctc gtcaagcgcg ggttccgctt catcgggccc
    14821 acgatctgct acgcgttcat gcaggccacc ggcatggtga acgaccacat cacgacgtgt
    14881 ttccgctatc ccaggcgaag gagccaacgg tcatgaggat cgggatcatc ggggcaggcg
    14941 ctatcggttg cgtggtcggg gggctcctca ccaaggcggg gcacgacgtc acgctcgtgg
    15001 accagtggcc cgagcacatc gagaccatga agcagcgggg gctccggctc agcgggacct
    15061 gcggggagca cacgatcccg gtgaaggcac tccacatcca cgaattgcag ggggtggcga
    15121 cgccgttcga ggcggtcttc gtgtcggtga agtcctacga caccgagtgg gcggcccaga
    15181 tggcgctggc ctatctcaag cagcccgacg gagtggtggt ggacttccag aacggggtca
    15241 acgacgagcg ggtggcctcg gtg
//
LOCUS       DASUFA010000324         6409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1211596, whole genome shotgun sequence.
ACCESSION   DASUFA010000324 DASUFA010000000
VERSION     DASUFA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211596"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFX87_11995"
     CDS             complement(<1..427)
                     /locus_tag="VFX87_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995691.1"
                     /translation="MRLNEILFPTDFSPASEAAGRIAREMALQAGARLHVLHVVPPVT
                     DPSLPAEHLARAGQSLADGLRVETVLLSGWAGRNIVDYAREKRVDLIVVGTHGRTGIT
                     HAILGSVAETVVRLAPCLVLTVPAALLAERSAVPLAPPGA"
     gene            complement(453..908)
                     /locus_tag="VFX87_12000"
     CDS             complement(453..908)
                     /locus_tag="VFX87_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995692.1"
                     /translation="MGFRRVVLGMDGSAHSRRAVAFLTRFQPPPGGRVTVVRIVEPVR
                     VPSMPLVPGAMRAQIVGQADAANRHLVAVARRQVEAAAAALAQAGWRARAVVRSGVPL
                     AGLLATVRAERADVLVLGARGAGAVTHFLLGSVAEAALKHAPVEVLIVK"
     gene            complement(928..1062)
                     /locus_tag="VFX87_12005"
     CDS             complement(928..1062)
                     /locus_tag="VFX87_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995693.1"
                     /translation="MGIRKSMGKNKKSKKRTEAVAVKRDEVRRKAATDRGKSRPQGMR
                     "
     gene            complement(1073..3166)
                     /locus_tag="VFX87_12010"
     CDS             complement(1073..3166)
                     /locus_tag="VFX87_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX4995694.1"
                     /translation="MSGRSLAPFLDPASVAVIGASRDPFKVGGSVVANLRSAGFRGRI
                     IPVNSRTDSVQGLSAVRALLDVEGAVDLAVIAVPAADVLPALKQCAAKKVGGAVVISA
                     GFRESGGEGVRREAELREWLRDQPIRVLGPNCLGWIRPARRLNVTFAPGMPEPGGIAF
                     VTHSGALAVAILDWARERRVGFSLFASLGNQADVNESDLLDAVAADPETRVVACYLEG
                     VADGLRFLGALRRAAAVKPVVLLKAGRSAEGARAVSSHTGALAGSDRAFDAAVRQAGA
                     LRVDTVEELFDVARGLASQPLPRGRRLVVVTNGGGLGIVATDAAEAAGLTVAPLDEGV
                     RRRLAEGLPAAAGLGNPVDLVGDADARRYSHALHTLGSDSADAMLVLLTPQAATDAAS
                     IARTIAGATRGWPTPVAAAFVGGARVAPGVRVLEEAGIPCYAFPESAVKMLAGMARLA
                     SRRAGLAVPPARTVDPARSRVWLAGLGPVAGRQLGMPELAPLLDAYGIPRLDARVAVS
                     AADAAAVAEEIGFPVALKIASPDITHKTEVGGVALGLRDARTAREAAEAILRRVAIER
                     PEATIRGLLVEAMAPAGKELLLGGVRDDQFGPLVMVGLGGIYVEVFADTAARLAPVSS
                     AEALVMLDELKMAPLLRGTRGEPPVNRDTLAAIVGRFSQLLVDLPELAEIEINPLTVG
                     PSGAVAVDARARLGR"
     gene            complement(3163..3585)
                     /locus_tag="VFX87_12015"
     CDS             complement(3163..3585)
                     /locus_tag="VFX87_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4995695.1"
                     /translation="MRPEVKGAGGSVGDWMSKAPMAVPEDTPIRDAVSLMQGAGVRHL
                     LVLDGPVLIGILSNRDLRRLVTRDPESPLLAGPVRSIMSEGPVSVAPDTPLTTAARLL
                     LEQRIGALPVRQGEQIVGIFTVPDALEALLAIAEASGA"
     gene            complement(3597..4037)
                     /locus_tag="VFX87_12020"
     CDS             complement(3597..4037)
                     /locus_tag="VFX87_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4995696.1"
                     /translation="MFHRIVVATDFSECAQGAWELARRIAAAPGSELVVTHVLTEVPL
                     YGEGVFNIETARKVREGARKWAEGALEAWVGKARAEGLSARAVVRTGVPHQEIVAVAQ
                     DERADLVVIGTTGRGGLNRALLGSVADRVVRLAPCPVLTVRELA"
     gene            4211..>6409
                     /locus_tag="VFX87_12025"
     CDS             4211..>6409
                     /locus_tag="VFX87_12025"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4995697.1"
                     /translation="MSRRPGDVVTWFLRNRTSLAFSLAAGLVLAIVIIALGARSLAEQ
                     YQTVRQTDQVLGELDGLLSAVIDAEAGQRGFLMTGDERYLTPSLAAFDTAAGHLEALR
                     NLTAGHTSQRRHVDALEPLVRDKLGELRDTVQLGRERRADVAVQRILTGRGLRLMEQI
                     RSTVAAMQAEERQIQAGQSTRYTRQARHVFLGLAALAAVTLGLLVVVLVEYRRRAGEI
                     RATRSALRSQVAEYQQTSALLASIVQSSDDAIIGLGLDGRVLTWNAGAEQLYAYREEE
                     VTGRPIDILSPDRHKDEVSASLSRVLRERAVERIETEQITGHGRRLDVFMTISPIRDA
                     ANEVVGASIVARDITARKVAEEVLRRTEATSRALLESVTESIVVTDAAGRIVLVNART
                     EAMFGYARAELIGQPVEALLPASLRDRHVTHRATYMAAPRIRSMGRGLDLAALKKDGT
                     EFPVEVSLSYVETDEGVRAIAFVTDISERVAFQKAARQADKLAALGTLSAGIAHEINN
                     PIGIITSRVEVMMLEADEEGFPDELRKDLEVILRHARRVATITQGLLSFARQSSGTRG
                     PVNLNQVTEEIVQLARKDMSRAQVQVTTKLDEPMPTIVADANAIGQVLLNLLTNAAGA
                     IVRVNDKAEKMFGYGRAELIGQPVEVLIPRRAREAHVAHRAGYMAAPRVRSMGRGLDL
                     AGVKKDGTEFPVEVSLSFVETDDGIRAIAFVTDISERLAFQRAARQADKLA"
BASE COUNT          956 a         2431 c         2224 g          798 t
ORIGIN      
        1 cggccccggg cggcgcgagg gggacggcgc tccgctccgc gaggagcgcg gccggcaccg
       61 tcagcacgag gcagggcgcc aggcggacca ccgtctcggc cacgctgccg aggatcgcgt
      121 gggtgatccc ggtgcgcccg tgggtgccca ccacgatgag atcgacgcgc ttctcgcgcg
      181 cgtagtccac gatgttccgg ccggcccacc cgctcagcag cacggtctcc acgcggaggc
      241 cgtcggcaag gctctgcccc gcccgcgcaa ggtgctcggc gggcagcgac ggatcggtga
      301 ccgggggcac cacgtggagc acgtggagcc gcgcgccggc ctgcaacgcc atctcccgcg
      361 cgatgcgtcc cgccgcctcc gatgccggcg agaagtcggt cgggaacagg atctcgttca
      421 gtctcatgct cgatctcctc gtggattgcc cctcatttga cgatcaggac ctcgaccggc
      481 gcgtgcttca gcgcggcctc cgccacgctc ccgagcagga aatgggtcac cgccccggcg
      541 ccccgcgcgc ccagcacgag cacgtcggcc cgctccgcgc gaaccgtcgc cagcagcccg
      601 gcaagcggca cgccgctgcg cacgaccgcg cgcgcccgcc agcccgcctg ggcgagcgcg
      661 gccgccgcgg cctcgacctg gcggcgggcc accgccacca gatgccggtt cgccgcgtcc
      721 gcctgcccca cgatctgggc ccgcatggcg cccgggacga gcggcatcga gggcacccgc
      781 accggctcga cgatgcgcac gaccgtcacg cggcctccgg ggggcggctg gaaccgggtc
      841 aggaaggcca ccgcgcgacg agaatgcgcg gacccgtcca tccccagcac gacacgacga
      901 aatcccattc gagcggccac ggcccgacta cctcatgccc tgcgggcgac tcttgccacg
      961 gtccgtcgcc gccttgcgcc ggacctcgtc tcgcttgacg gccaccgcct ccgtccgctt
     1021 cttcgacttc ttgttcttgc ccatgctctt ccggatcccc atccgtgcct ccctatcggc
     1081 cgagccgggc ccgcgcgtcc accgccaccg cgccggaggg tccgaccgtc aaggggttga
     1141 tctcgatctc cgccagctcg gggagatcca ccaggagctg cgagaaacgg ccgacgatcg
     1201 cggccagcgt gtcacggttc accggcggct cgccgcgcgt gccgcgaagc agcggtgcca
     1261 tcttgagctc gtcgagcatc accagggcct cggccgagga taccggagcc aggcgcgccg
     1321 cggtatccgc gaaaacctcc acgtagatcc cgccgagccc gaccatcacg aggggcccga
     1381 actggtcgtc gcgcaccccg cccagcagga gctccttccc ggcgggcgcc atcgcctcga
     1441 cgaggagacc ccggatcgtc gcctccggcc gctcgatcgc gacccgtcgg aggatggcct
     1501 cggcggcttc gcgcgcggtg cgagcgtcgc ggagccccaa agccaccccg cccacctcgg
     1561 tcttgtgggt gatgtcgggt gacgcgatct tgagcgcgac cgggaacccg atctcctccg
     1621 cgacggcggc agcgtcggcg gcgctgaccg ccacgcgcgc gtcgaggcgc gggatgccgt
     1681 aggcgtcgag cagcggagcc agctccggca tgccgagctg ccgccccgcc acgggcccga
     1741 gccccgccag ccagacgcgc gagcgagccg gatcgaccgt gcgcgcggga ggaacggcca
     1801 gcccggctcg gcgcgacgcg agccgcgcca ttccggccag catcttgacc gccgactcgg
     1861 ggaaggcgta acagggaatg ccggcttcct cgagaacccg tacgcccggc gccacgcgcg
     1921 cgccgcccac gaaggcggcc gcgaccgggg tgggccagcc acgcgtcgcg cccgcgatgg
     1981 tgcgggcgat gctcgcagcg tcggtcgcgg cctggggagt gaggagcacc agcatcgcgt
     2041 cggcgctgtc gctccccagg gtgtgcagcg cgtggctgta ccggcgcgcg tcggcgtctc
     2101 ccaccaggtc caccgggttg ccgagcccgg cggcggcggg caagccctcg gccagccgcc
     2161 gccggacgcc ttcgtcgagc ggcgcgacgg tgagcccggc cgcctccgcc gcgtccgtgg
     2221 ccacgatgcc gagcccgccg ccattggtca ccacgaccag gcgacggccg cgcggcagcg
     2281 gctggctggc cagcccgcgc gcgacgtcga acagctcctc caccgtgtcc acccggaggg
     2341 cccccgcctg ccggacggcc gcgtcgaacg cccgatccga gcccgcgagc gcgccggtat
     2401 gggaagacac ggcccgggcc ccctccgcag agcggcccgc cttcagcagg acgaccggtt
     2461 tcacggcggc ggcgcggcgg agggcaccga ggaagcggag tccgtcggcg acaccctcga
     2521 ggtagcaggc gaccacccgg gtctccggat cggcggccac cgcatcgagg agatcgctct
     2581 cgttcacatc ggcctggttg ccgagggagg cgaagagcga gaagccgacg cggcgttcgc
     2641 gcgcccagtc caggatcgcc accgccagcg cccccgagtg ggtgacgaag gcgataccgc
     2701 ccggctcggg catcccgggc gcgaacgtga cgttgagccg gcgggcgggc cggatccagc
     2761 cgaggcagtt ggggccgagc acccggatcg gctgatcacg cagccactcg cgcaactccg
     2821 cctcgcgccg cacgccttcg ccgccgctct cccggaagcc cgccgagatc accaccgcgc
     2881 ccccgacctt cttggccgcg cactgcttga gcgcgggcag gacgtccgcg gcgggaaccg
     2941 cgatcacggc caggtccacc gcaccctcga cgtcgagcag cgcgcgcacc gcggacagtc
     3001 cctgcaccga gtccgtccgg gagttgaccg ggatgattcg cccgcggaag cccgccgagc
     3061 gcagattggc cacgacgcta ccgccgacct tgaacggatc gcgggacgcg ccgatcaccg
     3121 ccacgctggc gggatcgagg aacggggcga gcgagcggcc gctcacgcgc ccgaggcctc
     3181 ggcgatcgcg aggagtgcct cgagcgcgtc gggcaccgtg aagatcccga cgatctgctc
     3241 gccctgccgg accgggaggg cgccgatccg ctgctcgagc agcagccgcg ccgccgtggt
     3301 cagcggcgtg tcgggcgcca cgctcaccgg gccctcgctc atgatcgagc ggacgggccc
     3361 ggcgagaagc ggcgactccg gatcgcgggt gacgagacgc cggaggtccc ggttggagag
     3421 gatgccgatc agcaccgggc cgtcgaggac gaggaggtgc cgcaccccgg cgccctgcat
     3481 gagcgacacc gcgtcccgga tcggcgtgtc ttcgggaacc gccatcggcg ccttgctcat
     3541 ccagtcgccg accgatcccc ccgccccctt cacctcgggt cgcatcgcgg gtcggctcag
     3601 gccagctcgc gaaccgtgag caccgggcac ggcgccagcc gcaccacgcg atcggccacg
     3661 ctgccgagaa gcgcgcgatt gagcccgccg cggccggtgg tgccgatcac gacgaggtcg
     3721 gcccgctcgt cctgggccac cgccacgatc tcctggtgcg gaacgccggt gcgcacgacg
     3781 gcccgcgcgc tgagcccctc ggcacgcgcc ttgcccaccc aggcttccag ggcgccctcg
     3841 gcccacttgc gggccccttc ccgaaccttc cgggcggtct cgatgttgaa gacgccctcg
     3901 ccgtacaggg gaacctccgt gagcacgtga gtgacgacca gctcgctgcc cggcgccgcc
     3961 gcgatccgcc tggccagctc ccatgctccc tgcgcgcact cggagaagtc cgtcgccacc
     4021 acgatgcgat ggaacatggt cacctcctgt cggggcagaa ctgcaacgca ggtgccagcc
     4081 gaaactccca tacattcaac aggttgcagg actggatccc acggcctcgc gcggaagccg
     4141 tgccccgctg aggcgccgcc gccccaacat cttgaagggg cgacgctcct ggcccacaat
     4201 gcaaatcggc atgtctcggc ggcccggcga cgtggtcacc tggttcctcc gcaaccgcac
     4261 gagcctcgcg ttctcgctgg ccgccggcct cgtgctggcc atcgtgatca tcgcgctcgg
     4321 cgcgcgcagt ctcgccgaac agtaccagac cgtgcgtcag accgaccagg ttctcggtga
     4381 gctggatgga ctcctctcgg cggtgatcga tgccgaggcc ggccagcgcg gctttctgat
     4441 gaccggcgac gagcggtatc tcaccccgtc tctcgccgca ttcgacaccg cggcgggcca
     4501 tctcgaggcc ctccgcaact tgaccgcggg ccatacgagc cagcggcggc acgtcgacgc
     4561 cctggagcca ctcgtcaggg acaagctcgg cgagctgcgc gacacggtcc agctcggccg
     4621 cgagcggcgg gccgacgtcg cggtacagcg aatcctgacc ggtcgcggcc ttcggctcat
     4681 ggagcaaatt cgatccaccg tcgccgccat gcaggcggag gagcgccaga tccaggccgg
     4741 gcaatcgacc cggtacacgc ggcaggcccg ccatgtgttc ctcgggctgg ccgcgctggc
     4801 cgcggtgacc ctcggccttc tcgtggtcgt cctcgtcgag tatcgccggc gcgcgggaga
     4861 gatccgggcc acccggagcg cactccggag ccaggtggcc gagtaccagc agacgagcgc
     4921 cctgctcgcg tcgatcgtgc agtcgtccga cgacgcgatc atcgggctgg ggctcgacgg
     4981 acgcgtgctc acctggaatg cgggcgccga gcagctgtac gcgtatcggg aggaggaggt
     5041 cacgggtcgg ccgatcgaca tcctgagccc ggaccgccac aaggacgagg tctcggccag
     5101 cctgagccgc gtcctgcgcg agcgggcggt cgagcgaatc gaaaccgagc agatcacggg
     5161 acacgggcgg cggcttgacg tcttcatgac gatctcgccg atccgcgacg ccgccaacga
     5221 ggtggtggga gcatccatcg tggcgcgcga catcaccgcg cgcaaggtgg ccgaggaggt
     5281 gctgcgccgc accgaggcca cgtcccgggc gctcctcgaa tccgtgaccg agagcatcgt
     5341 ggtcacggat gcggcgggcc gcatcgtgct ggtgaacgcc cggaccgagg cgatgttcgg
     5401 ctacgcgcgg gccgagctga tcgggcagcc ggtcgaggcg ctcctcccgg cctcgctccg
     5461 cgatcggcac gtcacccacc gcgccacgta catggcggcg ccgcggatcc gctcgatggg
     5521 ccggggtctc gacctggccg cgctcaagaa ggacggcacc gagttcccgg tggaggtgag
     5581 cctgagctac gtcgagaccg acgaaggcgt gcgcgccatc gccttcgtca ccgacatcag
     5641 cgagcgggtg gcattccaga aagccgcccg ccaggccgac aagctggccg cgctgggcac
     5701 cctgtcggcc ggcatcgccc acgagatcaa caaccccatc ggcatcatca cctcgcgcgt
     5761 cgaggtcatg atgctcgagg ccgacgaaga ggggttcccc gacgagctcc ggaaggacct
     5821 cgaggtcatc ctccggcacg cccggcgggt ggccacgatc acgcaggggc tcctgtcctt
     5881 cgcccgccag tcgtccggca cccgcgggcc cgtgaacctg aaccaggtca cggaggagat
     5941 cgtccagctc gcccgcaagg acatgagccg ggcgcaggtc caggtcacca cgaagctgga
     6001 cgagcccatg cccaccatcg tggccgacgc gaacgcgatc gggcaggtgc tccttaacct
     6061 gctcaccaac gccgcgggcg ccatcgtgcg ggtcaacgac aaggcggaga agatgttcgg
     6121 gtacgggcgc gccgagctga tcggccagcc ggtcgaggtc ctgatcccgc gccgcgcgcg
     6181 ggaggcgcac gttgcccacc gcgccggcta catggcggcg ccccgcgtcc ggtccatggg
     6241 ccgggggctc gatctggccg gggtcaagaa ggacggcacc gagttcccgg tggaggtgag
     6301 cctgagcttc gtggagaccg acgacggcat ccgcgccatc gctttcgtca ccgacatcag
     6361 cgagcgcctg gcgttccagc gcgcggcgcg ccaggccgac aagctcgcc
//
LOCUS       DASUFA010000325         8827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1120021, whole genome shotgun sequence.
ACCESSION   DASUFA010000325 DASUFA010000000
VERSION     DASUFA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120021"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..730)
                     /locus_tag="VFX87_12030"
     CDS             complement(170..730)
                     /locus_tag="VFX87_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995698.1"
                     /translation="MSPGGHLVTSAIACAAVYAGTESLALTAGLAAGGFLIDVDHMVD
                     YVLFERQRDLRPGSFLRYYLNGKAQRVVLVLHSYELLVLLAAVAWYTNVDWLWGWVFG
                     MLLHLPLDIVFNGKFASGGLVHFYSFIVRARAGFRADLFTDRPRRPVSAEGFWAAFFK
                     AGQVEDGASSDPAPRPEPRGAEPVRG"
     gene            complement(741..866)
                     /locus_tag="VFX87_12035"
     CDS             complement(741..866)
                     /locus_tag="VFX87_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995699.1"
                     /translation="MLKRLGRRLLELIDSVPLGWLAPARQPVPVRVRRYRNPYLR"
     gene            1000..2994
                     /locus_tag="VFX87_12040"
     CDS             1000..2994
                     /locus_tag="VFX87_12040"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HEX4995700.1"
                     /translation="MAGKTAVTGFDEILRSDPSVWEIRTAGAGPGGRLPLTAEMLLES
                     PSGDIFGLTQNAGMGWDPRELGRPQFLMLSTQGGIRASDGKPIALGYHTGHWEIGLLM
                     QAAAEELAALGGLPFAGFCSDPCDGRTQGTPGMMDSLAYRNDAAVVLRRLARSLPTRR
                     GVLGVATCDKGLPAMMMALAALRDLPCVLVPGGVTLPPTEGEDAGTVQSIGARFAHGE
                     LTLDQAADLGCRACGSPGGGCQFLGTAATSQVVGEALGLSLPHTALAPSGQPIWLDMA
                     RRSARALTSLAARGLTTRDIVTDAAVRNAMMVHAAVGGSTNLLLHLTAVAHAAGLRRP
                     VVQDWIDVNRQVPRLVDVLPNGPRNHPTVRVFLAGGVPEVMLHLRRRGLLDETCLTVT
                     GESLGRALDWWEGSERRTALRQLLMRQDGVDPDDVIMDPERARERGLTSTVTFPRGNL
                     APEGAVIKSTAIDPSVVDADGVYRKIGPARVHTTERAAIAAIKSTGPERIKPGDVLVL
                     ICRGPLGAGMEEIYQITSALKHLSWGKQVAVLTDARFSGVSTGACIGHVGPEALAGGP
                     IGRLREGDLVQIVVDRLRLEGSVDLIGAEGRTFGAAEGARVLAARGPRPDLAPDPGLP
                     DDTRLWAALQHAGGGAWGGCVYDVDAIVARLNHPSPRPLP"
     gene            complement(3007..3300)
                     /locus_tag="VFX87_12045"
     CDS             complement(3007..3300)
                     /locus_tag="VFX87_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX4995701.1"
                     /translation="MQAETTETPQVDELDATDVQVAPPWMTVLHNCDCHTFEEVVKQL
                     MKAIACSEERGWELAWQVHNTGRAVVKVGAETECVRVGNVLAAIGLVVTVTQS"
     gene            complement(3320..4516)
                     /locus_tag="VFX87_12050"
     CDS             complement(3320..4516)
                     /locus_tag="VFX87_12050"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4995702.1"
                     /translation="MTRPPLDGVRILAVSQFGAGPFGTQMLADLGADIIKIEDPGVGG
                     DSARYVPPFQGEADSPYFQSFNRGKRSVSLNLRHPDGQAVFHDLVRVSDAVFNNARGD
                     LPDKLGLTYDQLKEINPRVVCCSLTGYGRTGPRAAEPAFDYLVQGYAGYMAVTGEPDG
                     PPGKCGVSVIDFAGGYAAMVGLMVGLFDAQRTGVGRDVDVALLDTAVSMLSYFAVWTL
                     NRDWIAERTRSSAHQTLVPAQNFATRDGWIVIFCNKDKFWRDLVETLGAPELADDARF
                     RTFGDRFAHKDALLPLLQARFATRSTEEWLDRLRGRVPCAPVNDVRQALADPQVLARD
                     MIVEVEHPDFGALKEVRSPVRTAGEIRDPARAPRLGEHTAQILREILSYSDGTIARLR
                     ETGVIG"
     gene            complement(4518..5000)
                     /locus_tag="VFX87_12055"
     CDS             complement(4518..5000)
                     /locus_tag="VFX87_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4995703.1"
                     /translation="MSYGRYYEEFTVGQEFTHWPGRTITEADCTWFALLTMNQHPLHS
                     DAHYAQTHTQHKQRVVLGPLVYSVVIGMTVADISGRAIANLEVNELKHEKPTFIGDTL
                     YAKSRVLALRESRRGDRGIVTVATQGVNQRGETVCSYTRAVLVPRKNHATLGEGRLPY
                     "
     gene            complement(4997..7231)
                     /locus_tag="VFX87_12060"
     CDS             complement(4997..7231)
                     /locus_tag="VFX87_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4995704.1"
                     /translation="MSPRTASRAPAIVASVREFVERDVMAAAPALEHADRYPHELVNR
                     MKRLGLFGALVPTDYGGLGLDVTTYAQIIEELCRGFMSLAGVINSHTMAALIVLEHGT
                     EEQRLRLLPRFASGQARGGLCLTEPHAGSDVQAIRTVARREGDRYLLSGSKMFVTNGR
                     EGNTFALLALTDPAESPRHRGMSCFIVEKGAPGLEVVKSIAKLGYKGVDTAELVFDGF
                     PCPAANLVGGVEGRGFKHVMSGLEAGRINIAARAVGVAQAGLDEAMRAARALPEPPAA
                     LTDIATRVESARLITYWAAAMKDRGDRCDLEAGMAKLHASEAAQAVAVEAMKIEGVRA
                     QLASGLVERLYRDAPLMIIGEGTNEIQRLIICRNLLERYGERPGALTSRDELPEEQKQ
                     LVLAVRQAVEKDVVPVAAEHDQGRRDPEPLVAQLAELGIFGALADTEHGGLGLPLTTA
                     AMIVEEMARGSAALAIWLSAQLAATLAVTRSVPRDARERLLPPMTRGERWVAPVAGAS
                     IGARRAGADWVLTGSAPVVPNAGRAKLFLVNAALEDGGARCFMIESGRSGLTVGLASD
                     TIGLRGLGAADVVLVGVRLGATAALREGGETLLEAATNVGAAATAVGLAQAAFEAALR
                     YSQQRSTFGKPICQHQAVQLKLADMATAITAARLLTGRAAARLDADARDDTGAAMACL
                     AAGETAYMVTLEAMRIHGGYGYTSEFPVERYYRDAAALLVGPRDQAGERRRIAARRLQ
                     APAP"
     gene            7349..8041
                     /locus_tag="VFX87_12065"
     CDS             7349..8041
                     /locus_tag="VFX87_12065"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4995705.1"
                     /translation="MTLTVASYNIHKCAGLDRRVDLDRIAQVLEEIDADLVGVQEVFR
                     PQALGLAERLGMEVAMGPTRERDGLPYGNAVLTRLAIRGSRTFDLSRPDREPRGGIRL
                     DLEMPMSEGLLHVFNVHFGLKIRERAEQVRMLVREHILHDELTGPRIVVGDLNEWFPG
                     AVGRTLRRELSGPRIRRTHPAPLPIFPLDRIYWDRGLGADGFHVHRSRLARVASDHLP
                     VVARLRLSSATA"
     gene            8193..8657
                     /locus_tag="VFX87_12070"
     CDS             8193..8657
                     /locus_tag="VFX87_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995706.1"
                     /translation="MEEHGTKREDVVALIGASPAAWQAHATEPAPTRLPSRALVVDDS
                     LVVAEVVTTLLRRTGWTVDVATSVDAALEHVRGVPYDLVVCDVCMPDGGGPAVYYAAT
                     TRRPDLTNRFLFITGNVDDPEPWRFLAKIRAHVLEKPFTGRALRHALIKVIA"
BASE COUNT         1235 a         3107 c         3229 g         1256 t
ORIGIN      
        1 cgagtgggcc cgagatcgcg gaccaggccg aggtgggggc cgtcgagatc gtcgagttcg
       61 tctggttctc gccgagcaag ggccggacgc tgacgacgcc gaacaaggcg gcgccgatgg
      121 tggacgggat cgacgactgg atgctgctga gcgagccgcc ggccgataac tagccgcgga
      181 ccggttcggc cccgcgcggc tccgggcggg gggccggatc gctcgacgcg ccgtcctcga
      241 cctggcctgc cttgaagaac gcggcccaga agccttcggc ggacaccggt cgccgcgggc
      301 gatcggtgaa gagatcggcg cggaatcccg cgcgggcccg cacgatgaag ctgtagaagt
      361 ggacgaggcc gcccgaggcg aacttgccgt tgaagacgat gtccagcggc aggtgcagca
      421 gcatgccgaa gacccatccc cagagccagt cgacgttggt gtaccacgcg accgcggcca
      481 gcaggaccag cagctcatag gagtgcagca ccaggaccac ccgctgcgcc ttgccgttga
      541 gatagtagcg gaggaagctg ccgggacgga ggtcgcgctg acgctcgaag agcacgtagt
      601 cgaccatgtg gtcgacgtcg atcaggaagc caccggccgc caggcccgcg gtgagtgcga
      661 ggctctcggt gcccgcgtag acggctgcgc aggcgatcgc ggaggtgacg agatggccgc
      721 cggggctcat gatgagccgc ctagcgcagg tacgggttgc ggtaccggcg aacgcgaacc
      781 gggaccggct gccgcgccgg cgcgagccac ccgagcggca cgctatcgat cagctccagc
      841 aggcggcgtc cgagtcgctt cagcatgcct ccgggaggga gcaactacgg tgccaggctc
      901 ggcgtgtcgc aagtcattga attatcgatg gacccccggg gccgggcgtg gaacagtagg
      961 ccaaaacagg gagccatgag gaccaaaatg gggagagtga tggcagggaa gaccgccgtg
     1021 accggcttcg acgagatcct ccgatccgat ccgtcggtct gggagatccg gaccgcgggc
     1081 gcgggccccg ggggccggct tccgctgacc gcggagatgc tgctcgagag cccgagcggg
     1141 gacatcttcg ggctcaccca gaacgcgggc atgggctggg atccgcgcga gctcggccgc
     1201 ccgcaattcc tcatgctctc cacccagggg ggtatccgcg cctccgacgg caagccgatc
     1261 gcgctcggct accacaccgg ccactgggag atcgggctgc tcatgcaggc ggccgccgag
     1321 gagctggccg cgctcggtgg cctgccgttc gccggtttct gctcggaccc ctgcgatggg
     1381 cgcacccagg gcacccccgg catgatggac agcctcgcct accgcaacga cgcggcggtg
     1441 gtgctgcggc ggctcgcccg ctcgctgccc acgcggcggg gcgtgctcgg cgtggcgacc
     1501 tgcgacaagg ggctgcccgc gatgatgatg gccctggccg cgctgcgcga tctgccctgc
     1561 gtgctggtgc cggggggcgt cacgctgccg cccaccgagg gcgaggacgc gggcaccgtg
     1621 cagtccatcg gggcccgctt cgcccacggt gagctcacgc tggatcaggc ggccgacctg
     1681 ggctgccgcg cgtgcggctc gcccggcggc ggctgccagt tcctcggcac cgcggccacg
     1741 tcccaggtcg tcggcgaggc gctcggcctc tcgctcccgc acaccgcgct cgcgccctcc
     1801 gggcagccga tctggctcga catggcccgg cgctcggccc gcgcgctgac gtcgctcgcc
     1861 gcgcgcgggc tcaccacgcg cgacatcgtc accgacgccg cggtgcggaa cgccatgatg
     1921 gtgcacgcgg cggtgggtgg ctcgaccaat ctcctgctgc acctcaccgc cgtcgcgcac
     1981 gccgccgggc tgcgccgccc ggtggtgcag gactggatcg acgtcaaccg gcaggtgccg
     2041 cgcctggtgg acgtgctgcc caacgggccc cgcaatcatc ccaccgtgcg cgtcttcctg
     2101 gccggcggcg tgcccgaggt catgctgcac ctgcgccgcc gcggcctgct ggacgagacc
     2161 tgcctgaccg tgaccggcga gtcgctgggc cgcgcgctcg actggtggga aggctccgag
     2221 cggcgcaccg cgctgcgcca gctgctgatg cggcaggacg gggtggaccc ggacgacgtc
     2281 atcatggatc cggagcgggc ccgcgagcgg gggctcacca gcaccgtgac cttcccccgc
     2341 ggcaacctgg cgccggaggg tgcggtgatc aagagcaccg ccatcgatcc gtcggtcgtg
     2401 gatgcggacg gggtctatcg caagatcggc ccggcccgcg tgcacacgac cgagcgtgcg
     2461 gcgatcgcgg ccatcaagag cacggggccc gagcgcatca agccgggcga cgtgctggtg
     2521 ctcatctgcc gcgggccgct cggcgcgggg atggaggaga tctaccagat cacctccgcg
     2581 ctcaagcacc tgtcatgggg caagcaggtg gcggtgctga ccgacgcgcg cttctccggc
     2641 gtctccaccg gcgcgtgcat cggccacgtc gggccggagg cgctcgcggg cgggcccatc
     2701 gggcgcctgc gcgagggcga cctcgtgcag atcgtggtgg atcggctgcg gctcgagggc
     2761 agcgtggatc tgatcggcgc cgagggccgg acattcgggg cggcggaggg agcgcgggtc
     2821 ctcgcggcgc ggggcccgcg gcccgatctg gcgcccgacc ccggccttcc ggacgatacg
     2881 cggctctggg ccgcgctgca gcacgcgggc ggcggggcct ggggcggctg cgtctacgac
     2941 gtggacgcga tcgtggcccg actgaaccac ccctcacccc gccctctccc ctgaggggag
     3001 agggagtcag gactgggtca cggtgacgac gaggccgatc gccgccagga cattgccgac
     3061 gcgcacgcat tcggtctcgg cgccgacctt caccaccgcc ctgccggtgt tgtgcacctg
     3121 ccacgccagc tcccagcccc gctcctccga gcaggcgatg gccttcatga gctgcttcac
     3181 cacttcctcg aaggtgtgac agtcgcagtt gtggagcacc gtcatccagg ggggcgcgac
     3241 ctggacgtcg gtcgcgtcga gctcgtcgac ctgtggggtt tcggtcgtct cagcctgcaa
     3301 ccggggcatc ctgtcttgtc tagcctatga ctccggtttc tcgaagacga gcgatcgtgc
     3361 cgtcgctgta gctcaggatc tcgcggagga tctgagcggt gtgctcgccg agccgcggcg
     3421 cccgtgcggg gtctcgaatc tcgcccgcgg tgcggaccgg gctccgcacc tccttcaagg
     3481 ccccgaagtc cgggtgctcc acctccacga tcatatcgcg cgccaggacc tgcgggtcgg
     3541 cgagagcctg gcgcacgtcg ttgaccggcg cgcacggcac gcggccccgc aggcggtcga
     3601 gccattcttc cgtactgcgg gtggcgaagc gggcctgcag cagcggcagc agcgcgtcct
     3661 tgtgggcgaa ccgatcgccg aaggtgcgga agcgcgcgtc gtcggccagc tcgggggcgc
     3721 ccagggtctc caccaggtcg cgccagaact tgtccttgtt gcagaagatg acgatccagc
     3781 cgtcgcgggt ggcaaagttc tgggcgggca cgagggtctg gtgcgccgag ctgcgggtgc
     3841 gctccgcgat ccagtcacgg ttgagcgtcc acaccgcgaa gtacgacagc atgctcaccg
     3901 cggtgtcgag cagcgcgacg tccacgtcgc ggcccacccc ggtgcgctgg gcatcgaaga
     3961 gccccaccat caggccgacc atcgcggcgt agccacccgc gaagtcgatc accgagacgc
     4021 cgcacttgcc cggagggccg tcgggctcgc cggtcaccgc catgtagccc gcgtagccct
     4081 ggaccaggta gtcgaaggcg ggctcggccg cgcgggggcc ggtgcgcccg tagccggtga
     4141 gcgagcagca gaccacgcgc gggttgatct ccttcagctg gtcgtaggtc aggccgagct
     4201 tgtcgggcag gtcgccgcgc gcgttgttga agaccgcgtc cgagacgcgc acgaggtcgt
     4261 ggaagaccgc ctgcccgtcg ggatgccgca ggttgagcga caccgagcgc ttgccgcgat
     4321 tgaaggactg gaagtagggc gagtcggcct cgccctggaa cgggggcacg tagcgggcgc
     4381 tgtcgccgcc gacgcccgga tcctcgatct tgatgatgtc ggcgcccaga tccgccagca
     4441 tctgggtgcc gaacgggccg gccccgaact gcgacacggc caggatacgc acgccgtcga
     4501 gcgggggccg ggtcatgtca gtagggaagc cggccctcgc cgagggtcgc gtggttcttc
     4561 cgggggacga gcacggcgcg ggtataggag cacacggttt cgcctcgctg gttgacgccc
     4621 tgggtggcca ccgtgacgat gccgcggtcg ccccggcgcg actcccgcag cgcgagcacg
     4681 cggctcttcg cgtagagggt gtcgccgatg aaggtgggct tctcgtgctt gagctcgttg
     4741 acctcgaggt tcgcgatggc ccgccccgag atgtcggcca ccgtcatgcc gatcaccacg
     4801 ctgtagacca gcgggcccag caccacgcgc tgcttgtgct gggtgtgggt ctgggcgtag
     4861 tgggcgtccg agtgcagcgg atgctggttc atggtgagca gcgcgaacca cgtgcagtcc
     4921 gcctcggtga tggttcgccc cggccagtgg gtgaactcct ggccgaccgt gaattcttca
     4981 tagtagcggc cgtagctcat ggcgcgggcg cctggagccg ccgcgcggcg atccggcgcc
     5041 gctcgcccgc ctggtcgcgc gggccgacca gcagcgcggc cgcgtcccgg tagtagcgct
     5101 ccaccgggaa ctcgctcgtg tagccgtagc cgccgtggat gcgcatggcc tcgagcgtca
     5161 ccatgtaggc ggtctcgccc gcggccaggc acgccatcgc ggcgcccgtg tcgtcgcgcg
     5221 cgtcggcgtc gaggcgcgcg gccgcccgcc ccgtcaggag ccgcgccgcg gtgatcgcgg
     5281 tcgccatgtc ggccagcttg agctgtaccg cctggtgctg gcagatcggc ttcccgaagg
     5341 tggaccgctg ctgggagtag cgcagcgccg cctcgaaggc ggcctgggcc agccccaccg
     5401 cggtggcggc ggcgccgaca ttggtcgcgg cctcgagcag cgtctcgccg ccttcgcgga
     5461 gcgcggccgt ggcgccgagg cgcacgccga ccaggaccac gtccgccgcg ccgaggccgc
     5521 ggaggccgat ggtgtcggag gcgaggccca ccgtgaggcc ggaccgaccc gactcgatca
     5581 tgaagcagcg ggccccaccg tcctccagcg cggcgttgac caggaagagc ttggcccggc
     5641 ccgcgttggg cacgaccggt gcggacccgg tgaggaccca gtccgcgccc gcccgtcgcg
     5701 ccccgatgga cgcgccggcc accggcgcga cccagcgctc gccccgcgtc atcggcggca
     5761 gcagccgctc gcgggcgtcg cgcggcacgc tgcgcgtcac cgcgagcgtc gccgcgagct
     5821 gggcggagag ccagatggcg agcgcggccg agccgcgcgc catctcctcc acgatcatgg
     5881 ccgcggtggt gagcggcagg ccgagcccgc cgtgctcggt gtcggccagg gcgccgaaga
     5941 tgccgagctc ggcgagctgc gccaccaggg gctcgggatc gcgccggccc tggtcgtgct
     6001 cggcggccac cgggacgacg tccttctcga cggcctgccg cactgcgagc acgagctgct
     6061 tctgctcctc cgggagctcg tcacgcgagg tcagcgcgcc cggccgctca ccgtagcgct
     6121 cgagcaggtt ccggcagatg atgagtcgct ggatctcgtt ggtgccctcg ccgatgatca
     6181 tgagcggcgc gtcgcggtag agccgctcca cgaggccgga agcgagctgg gcgcgcacgc
     6241 cctcgatctt catggcctcc accgcgaccg cctgggccgc ctccgaggcg tggagcttgg
     6301 ccatgcccgc ctcgagatcg caccggtcgc cccggtcctt catggccgcg gcccagtagg
     6361 tgatgagccg ggccgactcc acccgcgtgg cgatgtccgt gagggcggca ggcggctccg
     6421 gcagggcgcg cgcggcgcgc atcgcctcgt cgagcccggc ctgggcgacc cccaccgcgc
     6481 gagccgcgat gttgatccgt ccggcctcga ggccggacat cacgtgcttg aagccgcgcc
     6541 cctccacccc gcccacgaga ttcgcggccg ggcaggggaa gccgtcgaac accagctccg
     6601 cggtgtccac gcccttgtag ccgagcttgg caatggactt gaccacctcg aggccggggg
     6661 cgcccttctc gacgatgaag caggacatgc cgcggtggcg gggggactcg gcggggtcgg
     6721 tcagggcgag cagggcgaac gtgttgccct cgcggccgtt ggtcacgaac atcttggagc
     6781 ccgagagcag ataccggtcg ccctcgcgcc gggccaccgt gcggatggcc tgcacgtccg
     6841 agccggcgtg gggctcggtg aggcagaggc cgccgcgggc ctgcccgctc gcgaagcgcg
     6901 ggagcaggcg gagccgctgc tcctcggtgc cgtgctcgag cacgatgagc gcggccatgg
     6961 tgtggctgtt gatgacgccg gccagggaca tgaagccccg acagagctcc tcgatgatct
     7021 gggcgtaggt cgtgacgtcg aggccgaggc cgccgtagtc ggtgggcacc agggcgccga
     7081 agaggcctag gcgcttcatg cggttcacca gctcgtgcgg gtaccggtcg gcgtgctcga
     7141 gggcgggggc cgccgccatc acgtcccgct ccacgaactc gcggacggag gccacgatgg
     7201 cgggcgcgcg gctggcggtg cgtggactca aggaaacctc gcgtcgagac tagcaaagcc
     7261 cgcgcattca ttgcaaggct tgccccccgc caggcccggc ccgcggcttc ttcttggtcg
     7321 cggttgcatg gcccggtatg ctgggggcat gactcttacc gtcgccagct acaatatcca
     7381 caagtgcgcg ggactcgacc gccgcgtgga cctcgaccgg atcgcccagg tactggagga
     7441 gatcgacgcc gatctggtgg gcgttcaaga ggtgttccgg ccccaggccc tgggcctggc
     7501 cgagcgcctc ggcatggagg tcgcgatggg acccacccgg gagcgggacg gcctgcccta
     7561 cggcaacgcg gtcctcaccc gtctggccat ccgcggcagc cgcacgttcg acctctcccg
     7621 gcccgatcgc gagcctcgcg ggggcatccg cctggatctc gagatgccca tgtccgaggg
     7681 gctgctgcac gtcttcaacg tgcatttcgg gctcaagatt cgcgagcggg ccgagcaggt
     7741 ccgcatgctc gtgcgggagc acatcctgca cgacgagctg accggcccac gcatcgtggt
     7801 gggcgatctc aacgagtggt ttccgggcgc ggtcgggcgc acgctgcgcc gagagctcag
     7861 cgggccccgc atccggcgca cgcatcccgc gccgctgccg atcttcccgc tcgaccgcat
     7921 ctactgggac cgcggcctgg gtgccgacgg cttccacgta caccgcagcc gtctggcccg
     7981 cgtcgcctcg gaccacctgc cggtggtggc gcgcctgcgc ctgagctctg cgaccgcatg
     8041 acggcctgac gacggattgt tgccgggtgg tgccgcgctt tgtatgcgga cggatcggtg
     8101 caatcttgca cggttgacga ataggctaag gagatcaatg acttaccggg gaccggtcag
     8161 tggcaagcgc cttgctcacc tacaggcctg tcatggaaga gcacgggacg aagagggagg
     8221 acgtcgtcgc gctgatcgga gcgtcgccag cggcctggca ggctcatgcg acggagccgg
     8281 cgcccacgcg gctgccctcg cgggccctgg tggtggacga ctcgctggtg gtcgccgagg
     8341 tggtgaccac gctgctgcgc cggacgggct ggacggtgga cgtggcgacg agcgtggacg
     8401 cggcgctcga gcacgtgcgc ggcgtgccct acgacctggt ggtctgcgac gtctgcatgc
     8461 cggatggcgg tgggccggcc gtctactacg cggcgaccac gcggcggccg gacctgacca
     8521 accgcttcct cttcatcacc ggcaacgtgg acgaccccga gccctggcgc ttcctggcca
     8581 agatccgcgc ccacgtgctc gagaagccct tcaccggccg ggcactccgg cacgccctca
     8641 tcaaggtcat cgcctaaccc ggccgctctt tgcggccctg ctgctgctgt ccgcactcgc
     8701 cggccccgtc tgggcgggcc cccccaccga gcagctccgc gcccacaccg acaaggtcgt
     8761 ccgtatcctg gaggaccccg cgctcaacca ggcgcagcgc cggaccgaga tccacgcggt
     8821 cgcgctc
//
LOCUS       DASUFA010000326         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1574544, whole genome shotgun sequence.
ACCESSION   DASUFA010000326 DASUFA010000000
VERSION     DASUFA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574544"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFX87_12075"
     CDS             complement(<1..245)
                     /locus_tag="VFX87_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX4995707.1"
                     /translation="MATTYRLTPSRRAANRLVRLLLKLGLMPGPAYLLTVPGRRTGRP
                     LSTPVTLVEEGEARWLVAPYGDVAWVRSARAAGQVTL"
     gene            complement(255..1535)
                     /locus_tag="VFX87_12080"
     CDS             complement(255..1535)
                     /locus_tag="VFX87_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX4995708.1"
                     /translation="MGAIVERAERCWSGGVEPREFWKATGASEEIAPGVFFVHAFANV
                     TVVRAGRGLVLVDTANYVGRDRTFAAVRAIDPGPVHAAIYTHGHADHAFGLPPFLAEA
                     AAKGWPRPQIVGHRSVAARFDRYRATLGYNGLINARQFSIAASWPTEYDYPDTVYDQA
                     LRLETDGVALELQHARGETDDATWVWWPERRILWTGDLFIWVAPNAGNPQKVQRYAAD
                     WAAALREMAARGAELLIPGHGMPIVGADRVRQALTDTAAWLEALVSQTLALMNAGATL
                     EEVLRAVKPPAHLAERRYLQAVYDEPDYVVRNLWRLYGGWYDGVPSHLKPAPEAELGR
                     EILTLAGGMDGLIARARALAVEGRLALAAHLIDWAVAAEPEHREAHAARAQIYERRAE
                     DARALMTRGIFSATARESADKSRDPLSPQGRGPG"
     gene            complement(1535..2806)
                     /locus_tag="VFX87_12085"
     CDS             complement(1535..2806)
                     /locus_tag="VFX87_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995709.1"
                     /translation="MARTRWGVLVVPALLYFLSYFHRVAPVVVAGDLMAAFAVSAATL
                     GALSAIYPYCFAAMGLPGGSLADFLGPRRTLALGGLSMSAGSVLFGLAPGFAAAFAGR
                     LLVGLGASVILIAGLRLAAEWFRTEEFGTVAGAAQSVGSVGAIAGTTPLALLVEQIGW
                     RQGFVTIGAATALLAVACFLTVRDRPQRPAVPGLRPRAAEPGLREIIAGIPSVITNPR
                     SWTVGLISGGIYGGFAAFVGLWGVPYLTQVYELPRVRAANLVGLSALGLLVGAPLIGW
                     LSDRIHRRRLPLRVVSGLNVFTWLALVAPATPIAPAFLPLICFLFGLGSSVVVLVFAA
                     IREVNDPRYVGVALGFHNLPTFLSFGIMQWVTGVVLDRHWDGALVAGARVYGHDAYRA
                     AFALCLLLAAGAFVSACLAPETRGRNIWSAA"
     gene            complement(2806..3261)
                     /locus_tag="VFX87_12090"
     CDS             complement(2806..3261)
                     /locus_tag="VFX87_12090"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX4995710.1"
                     /translation="MSEKTTLLSEAETEFTLFKRAIAGLDDAQMRQVWLGTWSARDIA
                     AHISGWHREMGPALERLARGERPIAEGVSYDDVDAWNGRFADAKKTAATADILKELDA
                     SHAAFMRAAATVPDERYVPGKTAHKIVDLNSRHHYQEHRGQIEAWRKAG"
     gene            complement(3278..3724)
                     /locus_tag="VFX87_12095"
     CDS             complement(3278..3724)
                     /locus_tag="VFX87_12095"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4995711.1"
                     /translation="MIEIRLPRVADEIGCRTLAATVIGEERAGAFIRSHLERHHLIVA
                     EAVGEVVGFLAYRSDWFQCTFVSLVVVRDDFRRKGIAREFFKSVEAVSPTPRLFSSTE
                     ETNAVSIQMHRALGFQPSGHIDNLPQGVRELLFYKRIPPRSLGSTS"
     gene            complement(3747..>4431)
                     /locus_tag="VFX87_12100"
     CDS             complement(3747..>4431)
                     /locus_tag="VFX87_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX4995712.1"
                     /translation="PPQVAALKKITSPSVANAIETFNVRPREQGNLSSDIKALFPAMG
                     PMVGYAVPCVIRAEQGPIEGHRASTYGWWEFVQTVPAPRVIVVHDLDDPRGQGAQWGE
                     VQANIHRALGCVGVVTDGSVRDLDEVRALGFQFCAAHVSVSHANVHMVDFGIPIKVGG
                     VLIKPGDLIHGDQHGVCTIPHEIAAKIPEAIAKVEADEREIISVCQAPGFTADKLKEL
                     FKRIRPGTY"
BASE COUNT          664 a         1557 c         1636 g          574 t
ORIGIN      
        1 cacagcgtga cctgccccgc ggcgcgggcg ctgcggaccc aggcgacgtc gccgtaggga
       61 gcgaccagcc agcgagcttc gccttcctcc accagggtca ccggtgtgga tagcgggcgg
      121 ccggtgcggc ggccggggac ggtcagcaga tacgcggggc cgggcatcag gccgagcttc
      181 agcagaagac ggacgagacg attcgcggcg cggcggctgg gggtgaggcg gtaggtggtg
      241 gccacgagca cccctcaccc tggccctctc ccctgagggg agagaggatc gcgggatttg
      301 tcggcggatt cgcgggcggt ggcggagaag atgccgcggg tcatgagcgc gcgggcgtcc
      361 tcggcgcggc gctcgtagat ctgggcacgg gcggcgtggg cctcgcggtg ctcgggctcg
      421 gccgcgacgg cccagtcgat gaggtgggcg gccagcgcca ggcgcccctc gacggccagc
      481 gcccgcgccc gcgcgatgag gccgtccatg ccgccggcca gcgtcaggat ctcgcggccc
      541 agctcggcct ctggcgccgg cttgagatgc gagggcacgc cgtcgtacca gccgccgtag
      601 agccgccaga gattgcggac gacgtagtcc ggctcgtcgt acacggcctg gaggtagcgg
      661 cgctcggcca ggtgggccgg cggcttcacc gcgcgcagca cttcctcgag cgtggccccc
      721 gcgttcatca gcgcgagcgt ctggctcacc agcgcctcga gccacgccgc ggtgtcggtg
      781 agcgcctgcc gcacgcgatc ggccccgacg atgggcatgc cgtggccggg gatgagcagc
      841 tccgcgccgc gagccgccat ctcgcgcagc gccgccgccc agtccgcagc gtagcgctgg
      901 accttctgcg ggttgcccgc gttgggcgcg acccagatga agaggtcgcc ggtccacagg
      961 atgcgccgct ccggccacca cacccacgtc gcgtcgtcgg tctcgccccg cgcgtgctgc
     1021 agctcgaggg ccacgccgtc ggtctcgagg cggagcgcct ggtcgtagac ggtgtccggg
     1081 tagtcgtact cggtgggcca cgaggcggcg atggagaact ggcgcgcgtt gatgagcccg
     1141 ttgtagccga gggtcgcgcg gtagcggtcg aagcgggcgg cgacgctccg gtgaccgacg
     1201 atctggggcc gcggccagcc cttggcggcc gcctcggcga ggaacggcgg caggccgaag
     1261 gcgtgatcgg cgtggccgtg cgtgtagatg gccgcgtgca cgggcccggg gtcgatcgcc
     1321 cgcaccgcgg cgaaggtgcg gtcgcgcccc acgtagttgg cggtgtccac cagcacgagg
     1381 ccgcggcccg cgcgcaccac cgtcacgttg gcgaacgcgt gcacgaagaa gacgccgggg
     1441 gcgatctcct cgctcgcgcc ggtcgccttc cagaactcgc gcggctcgac gccgcccgac
     1501 cagcaccgct cggcgcgctc gacgatggcg cccatcaggc cgcgctccag atgttgcggc
     1561 cgcgggtctc gggcgccagg caggcggaga cgaacgcgcc cgcggcgagc agcaggcaga
     1621 gcgcgaaggc cgcgcggtag gcgtcgtgcc cgtagacgcg cgcgcctgcc accagcgcgc
     1681 cgtcccagtg ccggtcgagc acgaccccgg tcacccactg catgatgccg aaggacagga
     1741 acgtgggcag gttgtggaag cccagcgcca cgcccacgta gcgcggatcg ttgacctcgc
     1801 ggatggccgc gaagaccagc accaccacgc tcgaccccag gccgaagagg aagcagatca
     1861 gcggcaggaa ggccggcgcg atgggcgtcg ccggggcgac gagggcgagc cacgtgaaga
     1921 cgttcaggcc gctcaccacc cttagaggca gccggcgccg gtgaatccgg tcggagagcc
     1981 acccgatcag cggcgcgccc accagcaggc ccagcgcgga cagcccgacc aggttggcgg
     2041 cccgtacgcg cggcagctcg tagacctggg tcagataggg cacgccccag agccccacga
     2101 aggcggcgaa gcccccgtag atgccgcccg agatgaggcc caccgtccac gaacggggat
     2161 tggtgatgac gctcgggatc cccgcgatga tctcgcgcag acccggttcc gcggcgcgcg
     2221 gccgtaggcc gggcacggcc gggcgctgcg ggcggtcgcg cacggtgagg aagcacgcga
     2281 cggccaggag cgcggtggcg gccccgatgg tgacgaagcc ctggcgccag ccgatctgct
     2341 cgacgagcag ggccagcggc gtcgtgcccg cgatcgcgcc gaccgagccc acgctctggg
     2401 ccgcgcccgc gacggtgccg aactcctcgg tgcggaacca ctccgcggcc aggcgtagtc
     2461 ccgcgatgag gatcaccgac gcgcccaggc cgacgagcag gcgccccgcg aaggccgccg
     2521 cgaagccggg cgccagcccg aagagcaccg agcccgcgct catgctgagg ccgcccagcg
     2581 ccagcgtccg gcgcgggccc aggaagtcgg ccaggctgcc gccgggcaac cccatcgccg
     2641 cgaagcagta cggatagatc gcggacagcg cgcccagggt ggccgcggag accgcgaagg
     2701 ccgccatcag gtcgcccgcc accaccaccg gggccacccg gtggaagtag gagaggaaat
     2761 agagcagggc ggggacgacc agcacccccc agcgcgtccg ggccactaac cggccttgcg
     2821 ccaggcctcg atctgccctc ggtgctcctg atagtgatgc cggctattga ggtccacgat
     2881 cttgtgcgcg gtcttgccgg gcacgtagcg ctcgtcgggc acggtggcgg ccgctcgcat
     2941 gaaagcggcg tgcgaggcgt cgagctcctt caagatgtcg gcggtggccg ccgtcttctt
     3001 cgcgtccgcg aagcggccgt tccaggcgtc cacgtcgtcg taggagacgc cctcggcgat
     3061 cgggcgctcg ccccgggcga gccgctcgag cgccggcccc atctcgcggt gccagcccga
     3121 gatgtgggcc gcgatgtcgc gcgcgctcca cgttccgagc cacacctggc gcatctgtgc
     3181 gtcgtcgagc cccgcgatcg cgcgcttgaa cagggtgaac tcggtctccg cttcgctcag
     3241 cagggtcgtc ttctccgaca tggccgcacg cctccgtcta gctggttgat cccagggatc
     3301 tgggcgggat gcgcttgtag aagagcagct cgcggacgcc ctggggcagg ttgtcgatgt
     3361 gaccactcgg ctgaaagccg agcgcgcggt gcatctggat ggacaccgcg ttggtctcct
     3421 cggtggacga aaagagacgc ggggtgggcg ataccgcctc caccgacttg aagaactcgc
     3481 gcgcgatgcc cttgcggcgg aagtcgtccc gcaccaccac caggctgacg aaggtgcatt
     3541 gaaaccagtc cgaccggtag gcgaggaagc ccacgacctc gcccacggcc tcggcgacga
     3601 tcaggtggtg gcgctcgagg tgggagcgga tgaacgcgcc ggcgcgctcc tccccgatga
     3661 cggtagcggc gagggttcgg cagccgatct catcggccac ccggggcagt cgaatctcga
     3721 tcatgggggc gcccgcgcgg cggcgctcag tacgtgccgg gacggatccg cttgaagagc
     3781 tctttgagct tgtcggcggt gaagcccgga gcctggcaca ccgagatgat ctcgcgctcg
     3841 tcggcctcca ccttggcgat ggcttccggg atcttcgccg cgatctcgtg cggaatcgtg
     3901 cacaccccgt gctgatcgcc gtggatcaga tcgcccggct tgatcaggac gccgcccacc
     3961 ttgatcggga tgccgaagtc caccatgtgc acgttggcgt gcgagacgga gacgtgggcc
     4021 gcgcagaact ggaagccgag cgcgcgcacc tcgtccaggt cccgcaccga gccgtcggtg
     4081 accaccccca cgcagccgag cgcccggtgg atgttggcct ggacctcgcc ccactgggcg
     4141 ccctggccgc gcggatcgtc gaggtcgtgc accacgatca cccggggagc cggaacggtc
     4201 tggacgaact cccaccagcc gtaggtgctg gcccggtggc cctcgatggg cccctgctcg
     4261 gcccggatca cgcaggggac ggcatagccc accatcggcc ccatcgccgg gaacagcgcc
     4321 ttgatgtccg aggacaggtt gccctgctcg cgggggcgca cgttgaaggt ctcgatggca
     4381 ttggccacgc tgggactggt gatcttcttg agggcggcga cttgcggcgg c
//
LOCUS       DASUFA010000327         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_175396, whole genome shotgun sequence.
ACCESSION   DASUFA010000327 DASUFA010000000
VERSION     DASUFA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175396"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VFX87_12105"
     CDS             complement(<1..1016)
                     /locus_tag="VFX87_12105"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX4995713.1"
                     /translation="MVSMETLVSLCKRRGFIFQSSEIYGGTGSCWDYGPLGVELKNKI
                     KQVWWRDFVQQRADMVGLDASILMHPTVWKASGHVDHFTDPMVDCHECRHRFRADQID
                     EIPWIHYCPATKGNKFTIPAGEACSHCQVRRSLCPDCGKGELTPPRQFNLMFKTFMGP
                     VEEDASVTYLRPETAQGIFVNFDNVLQSMRRKLPFGIGQIGKAFRNEITPGNFIFRTR
                     EFEQMEIEYFVNPHETVDGRPADEHWHDRWIADCMAWFQRYGLQADNLRLHEHERDEL
                     AHYSKRTADVQYRFPIGWSELMGIANRTDFDLKQHAKWSGKTLTYFDEERKEHVVPYV
                     IEPSA"
     gene            complement(1092..1880)
                     /gene="recO"
                     /locus_tag="VFX87_12110"
     CDS             complement(1092..1880)
                     /gene="recO"
                     /locus_tag="VFX87_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX4995714.1"
                     /translation="MALHKTRAVVIGRRAFGESDRLVDFYTREYGKVRGIARAARRPR
                     SRFGSALELFTLGEMVFFDGGRSELVQVDHFDIVRPFVRVREHLERLGRGAWAVEMVA
                     RLSADRDPHPALFALLVRALAALELSRRPARVSVCFGLRAVDLLGHRPRLDRCVACGR
                     PAPFPDAALDVTAGGLVCAGCRPGADAMPLSGGLLGTLTRLRALSWEEALRLNLAAEL
                     DAELVAVLEGLVARLMGRYPLSSRFLAQTRRSLSLVSEPTPPRP"
     gene            complement(1882..2790)
                     /gene="era"
                     /locus_tag="VFX87_12115"
     CDS             complement(1882..2790)
                     /gene="era"
                     /locus_tag="VFX87_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX4995715.1"
                     /translation="MMPPPHRAGFVALVGRPNVGKSTLLNRLVGEKMAIVSPRPQTTR
                     TRITGIRHLPGAQVIFVDTPGLHAGAGRLGQLMSKTAERAVEDVDLVCFVAEATEDPA
                     RLDRDALDRLKACRAPVFCCLNKTDLVSPKARLLPLIAAYRVAYPFAEIVPLSAERGE
                     HCDRLLDLIVAALPERPAYFPPDALTDQPETFWVAEAIREKIFRLTHQEVPYACAVRV
                     EELTERHRPECLYIRAIIFVERESQRGIVIGKGATMLKRIGAAARTDLERFFGIKVYL
                     DLTVRVRSDWRKDDKALREFGFLLTS"
     gene            complement(2787..3263)
                     /gene="ybeY"
                     /locus_tag="VFX87_12120"
     CDS             complement(2787..3263)
                     /gene="ybeY"
                     /locus_tag="VFX87_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX4995716.1"
                     /translation="MPVAVSNLQRRTRVSPERLRTVARRALAALGRADREVHVAVVDD
                     RRIRALNTRYLGIRRATDVLAFDLAGPGPGRLWGEVIVSAETAARQARRLGVPLAAEL
                     DLLVVHGLLHLAGYDDHEPRRARRMHERAREILSGPPGSRSPRSIPARLWAGLLEA"
     gene            complement(3278..4264)
                     /locus_tag="VFX87_12125"
     CDS             complement(3278..4264)
                     /locus_tag="VFX87_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX4995717.1"
                     /translation="MTEATGVTRRIVLPPDLTHLALLGRHDEHLRQLEAQYDVRITAR
                     GHDVTVRGEERQVLEAERVVRELVAMLRERPSLGAADVRSALRIAGHEPGADVKSVLA
                     DAIAVPSRRRFITPKTANQKRYLEAVRGHDLVIAIGPAGTGKSYLAVAMAISALLKRE
                     VARIVLTRPAVEAGERLGFLPGDLVEKVHPYLRPLYDALYDMLEPEKVSALTEKGAIE
                     IAPLAYMRGRTLNDAFIILDEAQNTTSEQMKMFLTRLGFNSKMVVTGDITQVDLPASR
                     PSGLIEVQTVLQGIDGIRFIYFDDRDVVRHDLVSSIVRAYDRVQKPSSRGAG"
BASE COUNT          670 a         1525 c         1601 g          617 t
ORIGIN      
        1 gcggagggct cgatgacgta gggcaccacg tgctccttgc gctcctcgtc gaagtaggtc
       61 agcgtcttgc cgctccactt cgcgtgctgc ttcaggtcga aatcggtgcg gttggcgatg
      121 cccatcagct cgctccagcc gatcgggaac cggtactgca cgtccgcggt gcgcttggag
      181 tagtgcgcca gctcgtcccg ctcgtgctcg tgcaggcgga ggttgtccgc ctgcagcccg
      241 tagcgctgga accaggccat gcagtccgcg atccagcgat cgtgccagtg ctcgtcggcg
      301 gggcgcccgt ccaccgtctc gtgggggttg acgaaatact cgatctccat ctgctcgaac
      361 tcgcgggtcc ggaagatgaa gttcccgggc gtgatctcgt tgcggaacgc cttgccgatc
      421 tggccgatgc cgaagggaag cttgcgccgc atggactgga gcacgttgtc gaagttgacg
      481 aagatgccct gggcggtctc cgggcgcagg taggtgaccg acgcgtcctc ctccaccggc
      541 cccatgaacg tcttgaacat gaggttgaac tgccgtgggg gcgtgagctc gcccttgccg
      601 caatcggggc acaagctccg ccgcacctgg caatgcgagc acgcctcccc ggcggggatg
      661 gtgaacttgt tccccttcgt ggccgggcag tagtggatcc acgggatctc gtcgatctga
      721 tcggcgcgga agcgatggcg gcactcgtgg cagtccacca tgggatcggt gaagtgatcg
      781 acgtgcccgc tcgccttcca cacggtgggg tgcatcagga tggaggcgtc gaggcccacc
      841 atgtccgcgc gctgctggac gaagtcgcgc caccacacct gcttgatctt gttcttcagc
      901 tcgaccccca gcgggccgta gtcccagcac gagccggtgc cgccgtagat ctcgctcgac
      961 tggaagatga acccgcggcg cttgcagagc gagacgagcg tctccatcga gaccatggcg
     1021 gtcaggatag cacagcggcg cgagggctcc ccctcacgct gccctctccc ccgaggggaa
     1081 agggagatag atcaggggcg aggcggcgtg ggctcggaca ccagcgacag cgagcggcgc
     1141 gtctgggcga gaaagcgcga ggacagcgga taccggccca tgagccgcgc caccaggccc
     1201 tccaggaccg cgaccagctc ggcgtcgagc tccgcggcca ggttgagccg aagcgcttcc
     1261 tcccaggaaa gcgcgcggag ccgggtcagg gtgcccagga gcccacccga gagcggcatc
     1321 gcgtcggccc ccggccggca gcccgcgcag accaggccac ccgcggtcac gtcgagcgcc
     1381 gcatcgggga acggggcggg ccgcccgcag gcgacgcatc ggtcgagccg cgggcgatgg
     1441 ccgaggagat ccaccgcccg gaggccgaag cacaccgaga cgcgcgcggg gcggcgggac
     1501 agctccagcg cggcgagcgc ccggaccagc agggcgaaga gggccgggtg cgggtcgcgg
     1561 tcggcggaga gacgcgccac catctccacc gcccacgcgc cccggccgag gcgctccagg
     1621 tgctcccgca ctcggacgaa cgggcgcacg atgtcgaagt ggtccacctg gaccagctcg
     1681 ctccgcccgc cgtcgaagaa caccatctcg ccgagcgtga acagctcgag cgcgctcccg
     1741 aagcgcgacc ggggccggcg ggccgcgcgg gcgatgcccc gcaccttgcc gtactcgcgg
     1801 gtgtagaaat ccacgaggcg gtcgctctcc ccgaaggcgc gccgcccgat caccaccgcg
     1861 cgggtcttgt ggagcgccat gtcaggacgt caggaggaag ccgaactccc ggagcgcctt
     1921 gtcgtccttg cgccagtccg agcggacgcg cacggtcaga tccaggtaga ccttgatgcc
     1981 gaagaagcgc tcgagatcgg ttcgcgcggc ggccccgatc cgcttgagca tcgtcgcgcc
     2041 cttgccgatc acgatccccc gctgggactc gcgctccacg aagatgatgg cgcggatgta
     2101 caggcactcg gggcggtggc gctcggtcag ctcctccacc cgcaccgcgc aggcgtaggg
     2161 gacctcctgg tgggtgagcc ggaagatctt ctcccggatg gcctccgcca cccagaacgt
     2221 ctccggctga tccgtgaggg cgtcgggcgg gaagtaggcc gggcgctccg gcagggccgc
     2281 cacgatcagg tcgagcaggc ggtcacagtg ctcgccgcgc tcggcggaga gcgggacgat
     2341 ctccgcgaac ggataggcga cccggtaggc cgcgatcagc ggcaggaggc gcgccttcgg
     2401 gctcaccagg tcggtcttgt tgaggcagca gaagaccggc gcgcggcagg ccttgagccg
     2461 gtcgagcgcg tcgcggtcga ggcgcgccgg gtcctcggtc gcctccgcca cgaagcagac
     2521 gaggtccacg tcctccaccg cgcgctcggc cgtcttcgac atgagctggc cgaggcgtcc
     2581 ggcccccgcg tgcaggcccg gcgtgtccac gaagatgacc tgggcgccgg gcagatggcg
     2641 gatcccggtg atgcgggtgc gggtcgtctg ggggcgcggc gagacgatgg ccatcttctc
     2701 gccgaccagg cggttgagca gcgtggactt gcccacgttg ggccgcccga cgagggccac
     2761 gaagcccgcc cgatgcggag gcggcatcag gcctcgagga gcccggccca gagccgcgcg
     2821 gggatcgagc gcggcgatct ggagccgggt gggccggaca ggatctcgcg cgcccgctcg
     2881 tgcatccggc gggcgcggcg cggctcgtgg tcgtcgtagc cggccaggtg cagaagcccg
     2941 tgcacgacca gcaggtccag ctcggcggcc agcggcaccc cgaggcgccg cgcctggcgc
     3001 gccgccgtct ccgcggacac gatcacctca ccccagagcc ggcccggacc gggaccggcc
     3061 aggtcgaacg cgagcacgtc ggtggcccgg cggatgccga gatagcgcgt gttgagcgcc
     3121 cggatgcgcc ggtcgtccac gaccgcgacg tggacctcac ggtcggcccg gccgagcgcg
     3181 gcgagggccc ggcgggccac cgttcgcagg cgctccggcg agacgcgggt cctccgctgc
     3241 agattggaga ccgcgaccgg cacgggcggg ccggacctca gcccgctccg cgcgacgacg
     3301 gcttctggac ccgatcgtag gcgcgcacga tggaggagac gagatcgtgg cgcacgacgt
     3361 cacggtcgtc gaagtagatg aagcggatgc catcgatccc ctgcagcacc gtctgcacct
     3421 cgatcaggcc ggacggccgg ctggcgggca ggtcgacctg ggtgatgtcc ccggtgacca
     3481 ccatcttgga gttgaagccc agccgggtca ggaacatctt catctgctcg gacgtggtgt
     3541 tctgggcctc gtcgaggatg atgaaggcgt cgttcagcgt gcgcccgcgc atgtaggcga
     3601 gcggcgcgat ctcgatggcg cccttctcgg tgagcgcgct caccttctcg ggctccagca
     3661 tgtcgtagag cgcgtcgtag agcgggcgga gatagggatg caccttctcc acgaggtcgc
     3721 cggggaggaa gcccaggcgc tcccccgcct ccaccgccgg ccgcgtcaac acgatgcgcg
     3781 cgacctcgcg cttcagcagg gcggagatcg ccatcgccac cgccagatat gacttcccgg
     3841 tgcccgcggg gccgatcgcg atgaccagat cgtgcccgcg caccgcctcc aggtagcgct
     3901 tctggttcgc ggtcttcggg gtgatgaagc ggcgtcgcga cggcaccgcg atggcgtcgg
     3961 ccagcaccga cttgacgtcg gcgcccggct cgtgccccgc gatgcgcagg gccgagcgga
     4021 cgtcggcggc cccgagcgag ggccgctccc gcagcatggc caccagctcc cgcaccaccc
     4081 gctccgcctc cagcacctgc cgctcctctc cgcgaaccgt cacgtcgtgg ccgcgcgcgg
     4141 tgatgcggac gtcgtactgg gcttcgagct ggcgcaggtg ctcgtcgtgg cggccgagaa
     4201 gggcgaggtg agtgaggtcc ggaggcagga cgatccgccg ggtgacgccg gtggcttcag
     4261 tcaacgaagg ctcattcctc tgcccgggag catatcacgc gggtccgagc ccttccacga
     4321 gacggatccc gagctcgcgg agctgcttgg ggtccaccgg cgcgggcgcg tcggtgagcg
     4381 ggcaggtgcc cttctgggtc ttggggaagg cga
//
LOCUS       DASUFA010000328         3782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1327421, whole genome shotgun sequence.
ACCESSION   DASUFA010000328 DASUFA010000000
VERSION     DASUFA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327421"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1171)
                     /locus_tag="VFX87_12130"
     CDS             complement(122..1171)
                     /locus_tag="VFX87_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX4995718.1"
                     /translation="MSLVVLDAGPLTTVQDLGRAGQLRVGIPPSGPMDRDAFVLANRL
                     VGNPDGAAGLECTLMGPRLQLADARWVAVTGAEVPVTLDGAEMPRWASFPVPAGGIVK
                     LGAARAGVRAYLAVSGGLATPLVLGSRATYLRGALGGLSGRALRKDDVLPLGVPAGRA
                     AERRVRAERIPTHGGEPAIATILGPQADRFTPAGIAALFEGPYEMLPQSDRMGARFKG
                     PRIEHTRGHDIISDGIALGAIQVIGDGQPIVLLVDRQTAGGYTKIGAVCSYEIGRVGQ
                     MKPGQKLRFKQVEVLEAHAVLAARRAALESAIAEPVTGRLHHDARIRHPRLSGQDAPL
                     PPALRGRPACHARRE"
     gene            complement(1168..1899)
                     /gene="pxpB"
                     /locus_tag="VFX87_12135"
     CDS             complement(1168..1899)
                     /gene="pxpB"
                     /locus_tag="VFX87_12135"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX4995719.1"
                     /translation="MIYPEPRLLPCGDLAVSVELADEINREVNARVLALEYLIQQKAV
                     PGVTETLPTYRSLLVYYDPAVIAVDELHATLRALAAEARPEVLPPARTVELPCAYGGE
                     LGFELETAATKLGLAPDEVVRLHAGAHYHVYFVGFTPGLPYMTGMPERLNIPRLVNPR
                     TKTPPGSVAIGGVQCCIYSVESPGGFWVLGRTPVRLYDPTAADPILLRAGDQVRFRAI
                     ERAEYDAIAAEVAAGTFRPRVTEAR"
     gene            complement(1916..2704)
                     /locus_tag="VFX87_12140"
     CDS             complement(1916..2704)
                     /locus_tag="VFX87_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEX4995720.1"
                     /translation="MPIDARHPKEIRADIRRGALTGVTAGLAPGFVQANLAILPRDAA
                     YDFLLFCQRNPRPCPLIEVTDMGSPEPVGVAPGADLRTGLPRYRIYKDGVMADEVTDI
                     TPFWRDDLVSFLLGCSFTFEWALLDAGIPMRHIEQGKNVAMWKTSVECRPAGRFHGPM
                     VVSMRPIPQSMLSKAVTASARFPNAHGAPVQIGDPAAIGIKDVTRPDWGDFVGIQAGE
                     VPVFWACGVTPQAVALASKPEFMITHSPGHMFVTDLPNHQLAAI"
     gene            complement(2718..3701)
                     /locus_tag="VFX87_12145"
     CDS             complement(2718..3701)
                     /locus_tag="VFX87_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX4995721.1"
                     /translation="MKPHIVVGGGLHADGLKLMEAEARVTLVSDAETEAGMVAAAKDA
                     AGILFRSKPLCSRSLMAALPNLKVIGRHGVGLDIVDLPAATDLGIAVVHAPGSNSNSV
                     AEHAIMLMLACAKQAVVVDQRTRKADWGKGRWETLFEMNGKTLGIIGVGNIGRRVAKT
                     AGALGMKVIGYDKYVAAEELRNRGVEPMPDMASVLRAADVVTCHTPHTKETHHMIDAA
                     AVAQMKPGVIFINTSRGKTQDESALLAGLESGKIRAAGLDVFEEEPVSSDNRLLQLDQ
                     VIVSPHIAGVTRETMRGMAIQVTEEMLRVLRGEKPHVLANPDCWPKLGHLK"
BASE COUNT          567 a         1302 c         1354 g          559 t
ORIGIN      
        1 gatgaggctg gtgtagttgc cgttgaagtc cagccgctcg ttgccatccg cgtcccagat
       61 ccgcgcccct gccccccggg tgatgtagaa gggatacggg tcgaagaagg tggtggtacg
      121 gctattcccg ccgggcatga cacgcagggc gtcctcgaag agctggcggg agcggggcgt
      181 cttggcctga tagtcgcgga tggcggattc gagcgtcatg gtgaagcctc ccggtgacag
      241 gttcagcgat cgcggattcg agcgcggcgc ggcgcgccgc caggacagca tgagcctcca
      301 acacctccac ctgcttgaag cggagcttct gcccgggctt catctgcccg acgcggccga
      361 tctcgtacga gcataccgcg ccgatcttcg tgtagccccc ggcggtttgc cggtccacca
      421 ggagcacgat gggctgtccg tcgccgatca cctggatcgc ccccagcgcg atgccgtccg
      481 agatgatgtc gtggccgcgc gtatgctcga tgcgcggccc cttgaagcgc gcgcccatcc
      541 ggtccgattg cggcagcatc tcatagggcc cctcgaagag ggcggcgatc ccggcggggg
      601 tgaagcgatc ggcctgggga ccgaggatgg tggcgatggc gggctcgccg ccgtgggtgg
      661 gaatgcgctc ggcgcgcacc cgtcgctccg ccgctcggcc cgccggcacg cccagcggca
      721 gcacgtcgtc ttttctgagc gcgcgcccgc tcaggccgcc gagcgcgccc cgcagatagg
      781 tcgcccgcga gccgagcacg agcggcgtgg ccagcccgcc ggagacggcg agataggcgc
      841 gcacgccggc gcgcgccgcg ccgagcttga caatgccgcc cgcgggcacc gggaaggagg
      901 cccagcgcgg catctccgcg ccgtccagcg tcacggggac ctcggcgccg gtcaccgcca
      961 cccagcgcgc gtccgcgagc tggagccgcg ggcccatgag ggtgcactcg agcccggcgg
     1021 cgccgtcggg gttgccgacc aggcgattgg cgagcacgaa ggcgtcccgg tccatcgggc
     1081 ccgacggcgg gatccccacc cggagctggc ccgcgcggcc cagatcctgg accgtggtga
     1141 gcgggcccgc gtcgagcacg acgaggctca tcgcgcctcc gtcacccgcg gccggaacgt
     1201 gcccgccgcc acctcggccg cgatggcgtc gtactcggcg cgctcgatgg cgcgaaagcg
     1261 cacctgatcg ccggcgcgca ggaggatggg atcggcggca gtgggatcgt agagccgcac
     1321 cggggtgcgg ccgagcaccc agaagccgcc cgggctctcc accgagtaga tgcagcactg
     1381 cacgccgccg atggccacgc tgcccggcgg cgtcttggtg cgcgggttga cgagacgggg
     1441 aatattgagc cgttcgggca tccccgtcat gtacgggagg ccgggagtga agccgacgaa
     1501 gtagacgtga tagtgggcgc cggcatgcag ccgaaccacc tcgtccgggg ccaggcccag
     1561 cttcgtcgcg gccgtctcga gctcgaaccc gagctcgccg ccataggcgc agggcagctc
     1621 cacggtccgt gcgggcggca gcacctcggg ccgcgcttcc gcggccaggg cgcggagggt
     1681 ggcgtggagc tcgtcgaccg cgatgacggc gggatcgtag tagacgagca gcgaccggta
     1741 ggtcggcagc gtctcggtga ctccggggac ggctttctgc tggatcagat actccagcgc
     1801 cagcacccgg gcattgacct cgcggttgat ctcgtcggcc agctccaccg acacggcaag
     1861 gtcgccgcag ggcagcaggc gcggctcggg atagatcacc cgggcgggcc tcgccctaga
     1921 tcgccgccag ctggtgattc ggcaggtcgg tgacgaacat gtgcccgggg ctatgcgtga
     1981 tcatgaactc gggcttggac gccagcgcca ccgcctgggg cgtcacgccg caggcccaga
     2041 acaccggcac ctcacccgcc tggatgccca cgaagtcgcc ccagtcgggc cgggtcacgt
     2101 ccttgatgcc gatggcggcg ggatccccga tctggaccgg cgcgccgtgg gcattgggaa
     2161 agcgcgcgga ggcggtcacc gccttggaga gcatgctctg ggggatcggg cgcatcgaga
     2221 ccaccatggg cccgtggaag cgtccggccg gccggcactc caccgacgtc ttccacatcg
     2281 ccacgttctt gccctgctcg atgtggcgca tgggaatgcc ggcgtcgagc agcgcccact
     2341 cgaaggtgaa ggagcagccg aggaggaagg acaccaggtc gtcgcgccag aagggcgtga
     2401 tgtcggtgac ctcgtcggcc atgaccccgt ccttgtagat gcggtagcgc gggagacccg
     2461 tccggagatc cgcgcccggg gccaccccca ccggctcggg cgagcccatg tcggtcacct
     2521 cgatgagcgg gcaggggcgc gggttgcgct ggcagaagag gaggaaatca taggccgcgt
     2581 cccggggaag aatggcgaga ttggcctgga cgaagccggg ggccagcccc gcggtcacgc
     2641 ccgtgagcgc cccgcggcga atgtccgccc ggatctcttt cggatggcgc gcgtcgattg
     2701 gcatggtggt ctccagccta cttgaggtgg ccgagcttcg gccagcagtc ggggttggcg
     2761 agcacgtgcg gcttctcgcc gcgcagcacg cggagcattt cctcggtcac ctggatggcc
     2821 attccccgca tggtctcgcg ggtcaccccg gcgatatgcg gcgacacgat gacctggtcc
     2881 agctggagca ggcggttgtc cgaggacacc ggctcctcct cgaagacgtc gagcccggcg
     2941 gcgcggatct tgccgctctc gagtcccgcc agcagcgccg actcgtcctg ggtcttgccg
     3001 cgcgaggtat tgatgaagat gacgccgggc ttcatctggg cgacggcggc ggcatcgatc
     3061 atgtggtgcg tttccttggt gtgcggcgta tggcaggtca ccacgtccgc cgcccggagc
     3121 accgaggcca tgtcgggcat gggctccacc ccgcggttcc ggagctcctc ggccgccacg
     3181 tacttgtcat agccgatcac cttcatgccg agcgcccccg ccgtcttcgc cacccgccgg
     3241 ccgatattgc ccaccccgat gatcccgagc gtcttgccgt tcatctcgaa gagcgtctcc
     3301 cagcggccct tgccccagtc cgccttgcgc gtgcgctggt ccaccaccac cgcctgcttg
     3361 gcgcaggcga gcatgagcat gatggcgtgc tcggccaccg agttcgagtt ggagccgggc
     3421 gcgtgcacca ccgcgatgcc gagatcggtg gcggcgggca ggtccacgat gtcgagcccc
     3481 accccgtgcc ggccgatcac cttgagattg ggcagggcgg ccatgagcga gcgcgagcag
     3541 agcggcttgg agcggaagag gatgcccgcc gcgtccttcg ccgccgccac catgccggcc
     3601 tcggtctccg catcggacac caaggtgacg cgcgcttccg cctccatcag cttgagccca
     3661 tccgcatgca ggccgccgcc gacgacgata tgtggtttca tgggatctcc tggaagatgg
     3721 gcttgccggg gaacacgagc cgcattatac cggaacggga ggttgcacag gatgacgacg
     3781 ct
//
LOCUS       DASUFA010000329         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_999361, whole genome shotgun sequence.
ACCESSION   DASUFA010000329 DASUFA010000000
VERSION     DASUFA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999361"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFX87_12150"
     CDS             complement(<1..236)
                     /locus_tag="VFX87_12150"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX4995722.1"
                     /translation="MTERDEVDLVPVKRRHGVPRALESCHTALVEGYVIEGHVPADLI
                     DRLLHERPKVVGLAVPGMPVGSPGMESPGRKPER"
     gene            complement(439..1599)
                     /locus_tag="VFX87_12155"
     CDS             complement(439..1599)
                     /locus_tag="VFX87_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX4995723.1"
                     /translation="MLNATRGKLLPTTVTGSYPRPAWFTEELHGRSFKDALGHAPFRE
                     QYLDAVSCLMAEQQRAGLDILTDGDSRFDLTVGGKSWFYYPIERLRGITGHADASSAG
                     DWKVLRPGRILYEVMEAYQPSIVGEKLAAGDLEYAALYKAAQRLTDRPVKFGAISAQT
                     LVKMLVNKHYPSERELIMEVADIFNVQLREVAAAGCQVIQLEEPRHHISGAAGVASDG
                     DLQFFTDAVNREIKGVETEVWLHTCWGNPNQQPLHWERPSYERALPYLLQTNADAIGL
                     ECASTNGRDLPLLGRYKTEKKIVLGVVSHTVTAVEPPEVVAGLIRKALEYVSVDRLIL
                     STDCGFGREGLSRRIAFYKSVAINLGANIVRRELKLPEVPIPAADPRFTFAG"
     gene            complement(1639..2478)
                     /locus_tag="VFX87_12160"
     CDS             complement(1639..2478)
                     /locus_tag="VFX87_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4995724.1"
                     /translation="MATAKLGDVELYYEITGEGTPLVWSHEFGGDHRSWEPQVRYFAR
                     RYQVVTYNHRGYPPSSVPKGAAGYSQDLLVDDLHRLLEHLGLGPVHLGGCSMGANVAR
                     DFALAHPEMLRSLIMVGAGAGAVNREQFLAGQAAIAAGLEREGQAHRLRGFETLATRT
                     SFKAKDPRGFAEFVRQAGEHDAQACAHLAREVMSKRKTVPEVAAGLRGLRVPTLIMVG
                     DLDAPCVEPSLMMRGLIPHAGLVVFPACGHTPNIEEPGLFNLHVTEFLAAVERGRWAG
                     WTA"
     gene            complement(2478..2972)
                     /locus_tag="VFX87_12165"
     CDS             complement(2478..2972)
                     /locus_tag="VFX87_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4995725.1"
                     /translation="MDAYRAVVDKRDQRAFLERPLPEESLRRILQAARMTGSSKNVEP
                     NRLIVVRDRARLQAMAAYGPFARFLGRAAATIVICQTQKHEFDAGRCAQNMMVAAWSE
                     GIGSCPAHLPEPELAKLLGIPSEIFINRVIGFGYVDPARAAAPASVGRPRRPLTELVH
                     WETW"
     gene            complement(2977..3471)
                     /locus_tag="VFX87_12170"
     CDS             complement(2977..3471)
                     /locus_tag="VFX87_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4995726.1"
                     /translation="MAPDAPGFDPLTHRFGEARYFEDMKVGEVFYLPSRTLTDANFAA
                     FQAVSGDNHPIHYDIEYCRRQGHPGLLAHGFQVLAQTAAGAGIFPHVVGESLIGFIEQ
                     SSRFLKPVYSGDTVYPLLTVTALTPGRKTGVITVRSTVHNQRRELCMEGEQKYLVRKR
                     HPGA"
BASE COUNT          541 a         1237 c         1342 g          533 t
ORIGIN      
        1 tagcgttcgg gcttccggcc gggtgactcc atgccgggcg agccgacggg catgccgggc
       61 accgcgagcc ccaccacctt cgggcgctcg tggaggaggc ggtcgatcag gtcggcgggc
      121 acgtggccct cgatcacata gccctcgacc agagcggtat gacaggactc cagcgcgcgg
      181 ggcacgccgt gtcgccgctt gacggggacg aggtcgacct catcgcgctc cgtcaccagg
      241 aagccgccgc tgcgcaggtg cacgacccac gcggtgcagc accctcaggt cgacaatcga
      301 tgaacggtga cgtggggctt ggcggcggcc cgcgcgcgtc cgccgcccag ggcagcgccg
      361 gccagggtga gggcgccggt gagaaatcct ctgcgcgtcg tcatatggtc tgtctcctcg
      421 ctccggatgt ccggaagcct atcctgcgaa ggtgaagcgt gggtcggcgg cggggatcgg
      481 cacctcgggc agcttgagct cgcgccgcac gatgttggcg ccgaggttga tcgccacgct
      541 cttgtagaag gcgatgcgcc gcgagaggcc ctcgcggccg aagccgcagt cggtggacag
      601 gatgagccgg tccacggaca cgtattccag cgccttgcgg atgaggcccg ccaccacctc
      661 gggcggctcc accgcggtga cggtgtggct caccaccccg agcacgatct tcttctcggt
      721 cttgtagcgc cccagcagcg gcaggtcgcg gccgttggtg ctcgcgcact cgagcccgat
      781 ggcgtcggca ttggtctgga gcagataggg cagggcgcgc tcgtaggagg ggcgctccca
      841 gtgcagcggc tgctggttcg ggttgcccca gcaggtgtgc agccacacct cggtctcgac
      901 gcccttgatc tcccggttca ccgcgtcggt gaagaactgg aggtctccgt ccgaggccac
      961 gcccgccgcc cccgagatgt gatgccgagg ctcctccagc tggatgacct ggcagccggc
     1021 ggccgccacc tcgcggagct ggacattgaa gatgtcggcc acttccatga tcagctcgcg
     1081 ctcggagggg tagtgcttgt tcacgagcat cttcaccagg gtctgggcgc tgatggcccc
     1141 gaacttcacc ggccgatcgg tgaggcgctg cgcggccttg tacagcgcgg cgtactcgag
     1201 atcgccggcg gcgagcttct cccccacgat ggacggctga tacgcctcca tcacctcgta
     1261 gaggatgcgg cccgggcgca gcaccttcca gtcgcccgcc gaggaggcgt ccgcgtgacc
     1321 ggtgatgccg cgcagtcgct cgatcggata gtagaaccac gacttgccgc ccacggtgag
     1381 gtcgaagcgc gagtccccgt cggtgaggat gtcgagcccc gcacgctgct gctccgccat
     1441 gagacaggac accgcgtcga gatactgctc gcggaagggg gcatggccca gcgcgtcctt
     1501 gaacgagcgg ccgtgcagct cctcggtgaa ccaggcgggg cggggatagg agccggtgac
     1561 ggtggtgggc aggagcttgc cgcgcgtcgc gttgagcatc gactttaccc tatcacgccc
     1621 gcgcggaacg gcgtcacctc aggccgtcca gcccgcccag cgcccgcgct ccaccgcggc
     1681 gaggaactcg gtgacgtgga ggttgaagag cccgggctcc tcgatgttcg gcgtatggcc
     1741 gcaggcgggg aagaccacca gccccgcgtg cgggatcagc ccccgcatca tgagggacgg
     1801 ctccacgcag ggcgcgtcca ggtcgcccac catgatgagg gtgggaacgc ggagcccgcg
     1861 gagccccgcc gccacctcgg gcacggtctt gcgcttggac atcacctcgc gcgcgagatg
     1921 ggcgcaggcc tgcgcgtcgt gctcgccggc ctggcgcacg aactcggcga agccccgcgg
     1981 gtccttggcc ttgaaggacg tccgggtggc gagcgtctcg aagccccgca gccggtgggc
     2041 ctgcccttcg cgctcgagcc ccgccgcgat ggccgcctgg ccggcgagga actgctcgcg
     2101 gttcaccgcg ccggcgccgg cgcccaccat gatgaggctg cgcagcatct ccgggtgcgc
     2161 cagcgcgaag tcgcgcgcca cattggcgcc catggagcag ccgcccagat gcacgggccc
     2221 gagacccagg tgctcgagca gccggtgcag atcatccacc aggaggtcct gggaatagcc
     2281 ggcggcgccc ttggggacgc tcgagggcgg gtagccgcgg tggttgtagg tcaccacctg
     2341 gtagcgccgc gcgaaatagc gcacctgcgg ctcccacgag cggtggtcgc cgccgaactc
     2401 gtggctccac accagcgggg tgccctcgcc cgtgatctcg tagtagagct cgacgtcgcc
     2461 cagcttcgcg gtcgccatca ccaggtctcc cagtgcacca gctcggtgag cggccgccgg
     2521 ggacgcccca ccgacgcggg agcggccgcc cgcgcgggat ccacatagcc gaagccgatc
     2581 acccggttga tgaagatctc ggagggaatc cccaggagct tggcgagctc gggctccggc
     2641 agatgcgcgg ggcaggagcc gatgccctcg gaccaggccg ccaccatcat gttctgggcg
     2701 cagcggccgg cgtcgaactc gtgcttctgg gtctggcaga tgacgatggt ggcggcggcg
     2761 cgcccgagga accgggcgaa ggggccgtac gcggccatcg cctgcagccg cgcgcggtcg
     2821 cgcacgacga tcaagcggtt gggctcgacg ttcttgctgc tcccggtcat gcgcgcggcc
     2881 tggaggatgc ggcgcagcga ctcctcgggg agggggcgct cgaggaacgc gcgctggtcg
     2941 cgcttgtcca ccaccgcccg gtaggcgtcc atgcgctcag gcgcccgggt ggcgcttccg
     3001 gacgaggtac ttctgctcgc cctccatgca cagctcgcgc cgctgattgt ggacggtgga
     3061 gcgcaccgtg atcacgccgg tcttgcggcc cggggtgagc gcggtgacgg tgagcagggg
     3121 atagacggtg tcgcccgagt acaccggctt gagaaagcgc gaggactgct cgatgaagcc
     3181 gatgagggac tcgcccacca cgtgcgggaa gatgcccgcg cccgccgcgg tctgggccag
     3241 cacctggaag ccgtgggcga ggaggcccgg gtgcccctgg cggcggcagt actcgatgtc
     3301 gtagtggatg gggtgattgt cgcccgacac cgcctggaag gcggcgaagt tggcgtcggt
     3361 gagcgtgcgc gaggggagat agaacacctc gcccaccttc atgtcctcga agtagcgcgc
     3421 ctcgccgaag cggtgcgtga ggggatcgaa gccgggcgcg tccggggcca tgggacgtcg
     3481 aggatagcgc ggcgacgcgg gcgcgacaag gccggcggcg cgtccgagga atctggcgaa
     3541 ggggccgtgc gcggccatcg cctgcagccg cgcgcggtcg cgcacgacga ccaggcgatt
     3601 gggctcgacg ttcttgctgc tcccggtcat ccgcgcggcc tggaggatgc ggc
//
LOCUS       DASUFA010000330         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_517526, whole genome shotgun sequence.
ACCESSION   DASUFA010000330 DASUFA010000000
VERSION     DASUFA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517526"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFX87_12175"
     CDS             complement(<1..409)
                     /locus_tag="VFX87_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4995727.1"
                     /translation="MTEHQAVDTLARMFWRRVERSGDRPAQMVKRGGGWETLDWSRVG
                     TQVREVALGLLALGLRAGDAVAILSRSRAEWVQADFAIFSAGGVTVPIYPSYTAEQVA
                     YIVNDAEARVLIVEDDAQLAKVLEVRGRLAGLGR"
     gene            complement(406..654)
                     /locus_tag="VFX87_12180"
     CDS             complement(406..654)
                     /locus_tag="VFX87_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX4995728.1"
                     /translation="MTRDEIRAVVLRTLGEIAPEADLATLEPDVSFRDQLDVDSMDLL
                     NFVIALHAVLHVDIPEVDYPKLATLDGCVEYLAGAGVR"
     gene            complement(651..1835)
                     /locus_tag="VFX87_12185"
     CDS             complement(651..1835)
                     /locus_tag="VFX87_12185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX4995729.1"
                     /translation="MAEFLMPILTADMSAGTLVEWRKKPGDALERGDIIALVETDKGL
                     IDIEVFTPGVLEKIVVQPGATVPTGAVLAIIREEAPTGMPAPAVAPPPPPPHPGPLPL
                     TGERGAAPVAPAPPGRLRISPLARKLAAELGVDPATVRGTGIGGAIRREDIEQAAAAR
                     AAAPAERPRAALDRHARLRQAIGAAMARSKREIPHYYLATTIDMHRAMTWLAEENLKR
                     PVTDRLLYGALLIKAVALALKEVPELNSVWRGSEPVRSEAVHVGVAISLRGGGLVAPA
                     LHDAAGQSLGDLMRQFRDLVQRARSGGLRSSELSDPTITVTSLGEQGVESVFGVIFPP
                     QVAIVGFGKIVERPWAVGGQLVARPVVTATLSADHRVSDGHRGGLFLAAVDRRLQEPQ
                     RL"
     gene            complement(1828..2811)
                     /locus_tag="VFX87_12190"
     CDS             complement(1828..2811)
                     /locus_tag="VFX87_12190"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX4995730.1"
                     /translation="MSKMSYRDAIRSALREALRDDPRVFLMGEDVGGYGGAFKVSYGL
                     LEEFGPERVRDTPLSENTFLGAGIGAALGGMRPIVEIMTVNFSLLALDQIVNNAATLR
                     HMSGGQLSVPLVVRMTTGGGRQLAAQHSHSLEGWFAHIPGIKIVAPAVAADARGFLLA
                     ALRDPDPVFIFEHATLYPVEGEVDEQAGPAEIGRALVRRAGDAVSVITYGGTLGKALQ
                     AADELAVDGIQAEVIDLRSLRPLDTETILASVARTHRAVIVDEGWRTCSFAAEVSARI
                     MEGGFYSLDAPVARVCSAEVPMPYAKHMEDAALPQVPAIVSAVMAMLGSHG"
     gene            complement(2808..>3308)
                     /locus_tag="VFX87_12195"
     CDS             complement(2808..>3308)
                     /locus_tag="VFX87_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4995731.1"
                     /translation="LNLAALWKLPVLFLCENNLYAMGTAVARHQSQPDIRRKADAYGI
                     ASDAADGMDVLAVEEATRRAADRVRRGEGPFLLELRTYRFRAHSTADPELYRTKEEVA
                     EWKRRDPIGLFTARLQAAGILSAADLASLEASVAADIAEAVGIAEAGPWEPVEDLTRD
                     VYTGTP"
BASE COUNT          454 a         1226 c         1169 g          459 t
ORIGIN      
        1 tgcggccaag cccggccagc cgcccgcgca cctccagcac cttggcgagc tgagcgtcgt
       61 cctcgacgat cagcacgcgt gcctcggcgt cgttgacgat gtaggcgacc tgctcggccg
      121 tgtagctggg atagatgggg acggtgacgc ctcccgccga gaagatcgcg aaatcggcct
      181 gcacccactc ggcgcgactc cgcgacagga tggccaccgc gtcgcccgcc cgcaggccca
      241 acgccaggag cccgagggcc acctcccgca cctgcgtgcc gacccggctc cagtcgagcg
      301 tctcccagcc gccgccccgc ttcaccatct gggccggacg gtcgccgctc cgctcgaccc
      361 ggcgccagaa catccgcgcg agcgtatcga cggcctgatg ctcggtcatc ggacacccgc
      421 tccggccagg tactccacgc agccatccag ggtggccagc ttcgggtagt ccacctccgg
      481 gatgtcgacg tggaggacgg cgtgcagggc gatgacgaag ttcagcaggt ccatcgagtc
      541 cacgtcgagc tggtcccgga aggacacgtc gggctcgagc gtggccagat ccgcctcggg
      601 cgcgatctcg ccgagcgtgc gcaggacgac ggcgcggatc tcgtcgcggg tcatagccgc
      661 tgcggctcct gcagccgccg gtccaccgcg gccaggaaga ggccgccgcg gtggccgtcg
      721 ctcacgcgat gatcggcgga gagcgtggcg gtgacgacgg gccgggccac gagctggccg
      781 cccaccgccc acggccgctc cacgatcttg ccgaagccga cgatggcgac ctgcggcggg
      841 aagatcacgc cgaagacgct ctccaccccc tgctcgccga ggctggtgac cgtgatggtg
      901 gggtcggaga gctccgagct ccgcagcccg ccgctccgcg ctcgctggac caggtcgcgg
      961 aactgccgca tgagatcgcc gagactctgg ccggccgcgt cgtggagcgc cggcgcaacg
     1021 agtcctcctc cgcgcagcga gatggccacc ccgacgtgaa cggcctcgct tcggaccggc
     1081 tcgctccccc gccacaccga gttgagctcc gggacctcct tgagcgcgag cgcgaccgcc
     1141 ttgatgagca gggctccgta gaggagccgg tcggtcaccg gtcgcttgag attctcctcc
     1201 gcgagccagg tcatcgcccg gtgcatgtcg atggtcgtgg cgaggtagta gtgggggatc
     1261 tcgcgcttgg agcgggccat ggcggccccg atcgcctgcc gcagccgcgc gtgccggtcg
     1321 agcgccgcgc gaggcctctc ggcgggagcc gcggcccggg cggcggccgc ctgctcgatg
     1381 tcttcgcgcc tgatggcgcc cccgattccg gtcccccgca ccgtggccgg atccacgccg
     1441 agctcggcgg cgagcttccg cgccagcggc gagatcctga gccggccggg cggggcgggc
     1501 gcgaccggcg cggcccccct ctcccccgtg agggggagag ggccggggtg agggggtggt
     1561 ggcggcggcg cgacggccgg cgcgggcatc ccggtcggcg cctcctcgcg gatgatggcc
     1621 agcaccgcgc cggtgggcac cgtggcgccc ggctgcacga cgatcttctc gagcacgccg
     1681 ggcgtgaaga cctcgatgtc gatcaggccc ttgtcggtct ccacgagggc gatgatgtcg
     1741 ccgcgctcga gcgcgtctcc gggcttcttg cgccactcga cgagcgtgcc ggcgctcatg
     1801 tccgccgtca ggatcggcat caggaactca gccatggctg cccagcatcg ccatgaccgc
     1861 gctcacgatg gccggcacct gcggcagcgc cgcgtcctcc atgtgcttgg cgtacggcat
     1921 cggcacctcg gcactgcaca cgcgcgcgac gggcgcgtcc aggctgtaga agccgccctc
     1981 catgatccgg gcgctcacct cggcggcgaa gctgcacgtg cgccagccct cgtccacgat
     2041 caccgcgcga tgggtcctgg ccaccgaggc gagaatggtc tcggtgtcca gcggccgcag
     2101 cgagcgcagg tcgatgacct cggcctggat gccgtccacc gcgagctcgt cggcggcctg
     2161 cagcgccttg ccgagcgtgc cgccgtacgt gatgacgctg accgcgtcgc ccgcgcgccg
     2221 caccagggcc cggccgatct cggcgggccc ggcctgctcg tcgacctccc cttccaccgg
     2281 atacagcgtc gcgtgctcga agatgaagac cgggtccgga tcgcggagcg cggccaggag
     2341 gaatccgcgg gcgtcggcgg ccaccgcggg cgccacgatc ttgatccccg ggatgtgggc
     2401 gaaccagccc tccaggctgt gcgagtgctg ggccgcgagc tgacggccgc ccccggtggt
     2461 catgcgcacc acgagcggca cgctgagctg gccccccgac atgtgccgga gcgtggccgc
     2521 attgttgacg atctggtcca gcgccagcag gctgaagttg acggtcatga tctccacgat
     2581 cgggcgcatg ccgccgagcg cggcgccgat accggcgccg aggaacgtgt tctccgacag
     2641 cggcgtgtcc cggacccgct ccggtccgaa ctcctcgagc agcccgtagc tgaccttgaa
     2701 ggcgccaccg tagccgccga cgtcttcccc catcaggaac acgcgcgggt cgtcgcggag
     2761 cgcctcgcgc agcgcggagc ggatcgcgtc ccggtagctc atcttgctca tggcgtcccc
     2821 gtgtagacgt cgcgggtcaa gtcctccacc ggctcccagg ggccggcctc ggcgataccc
     2881 acggcctcgg cgatgtccgc ggcgaccgag gcttccagcg aggccagatc ggcggcactc
     2941 aaaatgcccg ccgcctgcag ccgcgcggtg aacagcccga tgggatcgcg ccgcttccac
     3001 tccgcgacct cctccttcgt ccggtacagc tccggatcgg cggtggagtg ggcgcggaag
     3061 cgatacgtgc gcagctccag gaggaacggg ccctcgccgc gccgcacgcg gtcggcggcc
     3121 cgccgcgtgg cctcctccac cgccaggacg tccatgccgt cggccgcgtc cgacgcgatg
     3181 ccgtaggcgt cggccttccg ccggatgtcg ggctgagact ggtggcgggc caccgcggtg
     3241 cccatcgcgt agaggttgtt ctcgcagagg aagaggaccg ggagcttcca cagcgcggcc
     3301 agattgag
//
LOCUS       DASUFA010000331         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1212856, whole genome shotgun sequence.
ACCESSION   DASUFA010000331 DASUFA010000000
VERSION     DASUFA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212856"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFX87_12200"
     CDS             complement(<1..366)
                     /locus_tag="VFX87_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX4995732.1"
                     /translation="MSTLTIRTILAAALLIASTATAALAQSPPPGPGGPLGGMRGPMG
                     QTDGELWLMIRAAALTPDQQAKVRGILSTHRATTRPLIEQLRQAQQELGAKLLAPGPL
                     QSADLQPQLGRIGQLRDQLA"
     gene            complement(366..839)
                     /locus_tag="VFX87_12205"
     CDS             complement(366..839)
                     /locus_tag="VFX87_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995733.1"
                     /translation="MSRCLSDDALERVEAELAAVAERAHLAGCAACAARHRRMRGDLA
                     LIAGVLRDTPEPRTRPAPARRRRWMPATVGLAAVAAALLIWVEVAVWRAVTPVPPTMQ
                     PHEVAAILADVSAALFSVSGDPPAATAPAAATEDPAVNLEIMAQELDQTLTGGEP"
     gene            complement(836..1348)
                     /locus_tag="VFX87_12210"
     CDS             complement(836..1348)
                     /locus_tag="VFX87_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4995734.1"
                     /translation="MEPAPRQDLVVTAHYGRLQRLCRLLLGDPQKADDVVQDVFLKAH
                     EAERLGQVPASWGAWLTRVAVNGCRDRRRAGGWIRFRLRGARLDEVPLVALTPAPSDV
                     VISEDTRRRIWIAFRALPRRQQEVFALRHIEEQPTLTVAAVLGLSPGSVKRHLFRAVR
                     ALRRSLEDAR"
     gene            1651..1833
                     /locus_tag="VFX87_12215"
     CDS             1651..1833
                     /locus_tag="VFX87_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995735.1"
                     /translation="MIDKEFLAELVREPARVLATFDLTPDERAAIMHALGSTTNVSDR
                     DRARALQNVMLKRWAT"
     gene            1861..2361
                     /locus_tag="VFX87_12220"
     CDS             1861..2361
                     /locus_tag="VFX87_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4995736.1"
                     /translation="MASPPEIGFLRRVLYFKDVEEPVLLALWPSFRERRLKKGDVLFR
                     AGDPGEELFLIREGSIVVSKPVTGRVEQVLSRLASGEVFGEMSVFGDERRRSATCQAE
                     IDTMLYSLDRDSLNRFVTGSPLAAAKFFQQMAQVAFKRLRDSSDLVAEVTRWGLEATG
                     LDIEHK"
     gene            2380..>3143
                     /locus_tag="VFX87_12225"
     CDS             2380..>3143
                     /locus_tag="VFX87_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995737.1"
                     /translation="MSEVGEKTAEGRPKRTRIELLVREQDGDGFKTQEISSAVAYLHS
                     RPQRIPDADWEMARLPYAPQGVVTYALKNRRTEQIIQITETEKFLWGQMDGRASLQEI
                     ATSYLLNYGSFDFEIIPTLIVKLLRADLLTMRPVSRLRAVLARNRRNPAARAMEGTLH
                     ALERLTVTSRRSHQFFERMYRYGGFLLFSPWAIAALVLMVGLGIHSGIKIWHNLGEVT
                     HGLASRPIVAILLVKLVFWLTVINHQIIHALACIHY"
BASE COUNT          463 a         1106 c         1114 g          460 t
ORIGIN      
        1 cgcgagctgg tcgcggagct gaccgatgcg cccgagctgc ggctgcaggt cggcggactg
       61 gagcggcccg ggcgccagca gcttggcgcc cagctcctgc tgggcctggc ggagctgctc
      121 gatgaggggt cgcgtggtgg cgcggtgcgt ggacaggatc ccgcgcacct tcgcctgctg
      181 atccggcgtc agggcggccg cgcggatcat cagccacagc tcgccgtccg tttggcccat
      241 gggcccgcgc atgcccccga gcggaccgcc ggggccgggc gggggagact gcgcgagggc
      301 cgccgtcgcg gtcgaggcga tcaggagcgc ggcggcgaga atcgttcgaa tcgtcagcgt
      361 gctcatcatg gttctcctcc ggtgagcgtc tggtcgagct cctgcgccat gatctcgaga
      421 ttcacggccg gatcctccgt cgctgcggcg ggtgccgtcg cggcgggcgg atcgccgctc
      481 accgagaaca gcgcggccga gacgtcggcc aggatcgcgg ccacctcgtg cggctgcatg
      541 gtgggcggca ccggcgtcac cgcgcgccac accgcgacct cgacccagat cagcagggcg
      601 gccgcgacgg ccgcgagccc gacggtggcg ggcatccagc gtcgccgccg ggcgggggcc
      661 gggcgcgtcc ggggctcggg cgtatcgcgc agcacgccgg cgatcagggc caggtcaccg
      721 cgcatccgcc ggtggcgggc cgcgcacgcc gcgcagccgg ccagatgcgc ccgctcggcc
      781 accgcggcga gctccgcctc gacgcgctcc agcgcgtcgt cggagaggca gcggctcatc
      841 gggcgtcctc caggctccgg cgcagcgcgc gcaccgcacg gaagagatgg cgcttgacgc
      901 tgcccgggct gagcccgaga accgctgcga ccgtgagcgt gggctgctcc tcgatgtggc
      961 ggagcgcgaa gacctcctgc tgacgtcgcg gcagcgcgcg aaaggcgatc cagatgcgcc
     1021 gccgcgtgtc ctcgctgatg acgacgtcgc tgggcgcggg ggtcagcgcg accaggggca
     1081 cctcgtcgag gcgggcgccg cgcagacgga agcgtatcca gccgccggcc cggcgccggt
     1141 cgcgacagcc gttgaccgcg acccgggtga gccaggcgcc ccagctggcc gggacctgcc
     1201 cgagtcgctc ggcctcgtgc gccttcagaa agacatcctg caccacgtca tcggccttct
     1261 gcgggtcgcc caggaggagg cgacacaggc gttgcagccg cccgtagtgg gcggtgacca
     1321 ccagatcctg ccggggtgcg ggctccacgt ccctctgacg ggcgttccct cccctctcat
     1381 cagtcctgac gaacgaaatc gccgatccgt tgaccggtcg tggcagccct ggtgttctcg
     1441 gcccccggcg gcgtcttttt cggcctctac ccggtccgga aggccacccg ggtcgacccc
     1501 atcgacccgc tccggtccga gtgacggaac agaggtatta aaaccgcgcc gtgtatgcta
     1561 aattgacctc ctggtcatga tgcgcgtggc cggaaaagtc gaggtcgggg cgtggcgggc
     1621 aagggcattc aagacctagt cgggcaggtg atgatcgata aggagttcct tgccgagctc
     1681 gtgcgcgagc cggcgcgggt gctggccacc ttcgatctga ccccggacga gcgggccgcg
     1741 atcatgcacg ccctgggcag caccaccaac gtctcggacc gggatcgagc gcgcgccctg
     1801 cagaacgtca tgctgaagcg ctgggccacc tgaatgcggg tgcccgccgg acggagctga
     1861 gtggccagcc cccccgagat cggtttcctg cgccgggtcc tgtacttcaa ggacgtcgag
     1921 gagccggttc ttctcgccct ctggcccagc ttccgtgagc gtcgactcaa gaagggtgac
     1981 gtgctgttcc gcgcggggga tccgggcgaa gagctgttcc tgatccggga gggcagcatc
     2041 gtggtctcca agccggtcac cggccgggtg gagcaggtgc tctcgcggct cgcctccggc
     2101 gaggtgttcg gcgagatgag cgtcttcggc gacgagcggc gccgctccgc cacctgccag
     2161 gccgagatcg acacgatgct ctacagcctc gaccgcgaca gcctcaaccg cttcgtgacg
     2221 ggcagcccgc tcgccgcggc caagttcttc cagcagatgg cgcaggtcgc cttcaagcgg
     2281 ctgcgtgact cctcggacct ggtggccgag gtcacccgct gggggctgga ggcgacgggc
     2341 ctcgacatcg agcacaagta ggggatcggg agcccgaggg tgagcgaggt cggcgagaag
     2401 accgcggaag gcaggccgaa gcgcacgcgc atcgagctgc tggtccgcga gcaggacggc
     2461 gacggcttca agacccagga gatctccagc gcggtcgcct acctccacag ccggccccag
     2521 cgcatccccg acgcggactg ggagatggcg cggctgccct acgcgcccca gggcgtcgtc
     2581 acctacgcgc tgaagaaccg ccgcaccgag cagatcatcc agatcaccga gaccgagaag
     2641 ttcctgtggg ggcagatgga cgggcgcgcc tccctccagg agatcgcgac ctcgtacctg
     2701 ctcaactacg gctcctttga tttcgagatc atcccgacgc tgatcgtcaa gctgctgcgg
     2761 gccgacctgc tgacgatgcg gccggtctcg cgcctgcgcg cggtgctggc ccgcaaccgg
     2821 cgcaacccgg cggcccgggc gatggagggc acgctccacg cgctcgagcg cctcaccgtg
     2881 acgagccggc gctcgcacca gttcttcgag cgcatgtatc gctacggcgg cttcctgctc
     2941 ttctctccgt gggccatcgc cgcgctcgtg ctcatggtgg ggctcggcat ccacagcggg
     3001 atcaagatct ggcacaacct gggcgaggtg acgcacgggc tggccagccg tcccatcgtc
     3061 gccatcctgc tcgtgaagct cgtgttctgg ctcaccgtga tcaaccacca gatcatccac
     3121 gcgctcgcct gcatccacta caa
//
LOCUS       DASUFA010000332        14846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1454657, whole genome shotgun sequence.
ACCESSION   DASUFA010000332 DASUFA010000000
VERSION     DASUFA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454657"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFX87_12230"
     CDS             complement(<1..293)
                     /locus_tag="VFX87_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995738.1"
                     /translation="MAGARRALVAATLLLASGAGPVAAAPAPRLLAGAAAVTVELPGD
                     TPLAGYGGFPRRAWIPDLIGRFPDTFWFRPSTGVHDPIKVRALVLEGGGVRLL"
     gene            complement(293..1288)
                     /gene="holA"
                     /locus_tag="VFX87_12235"
     CDS             complement(293..1288)
                     /gene="holA"
                     /locus_tag="VFX87_12235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX4995739.1"
                     /translation="MDYAGFVRAADTGQVGPVTLLHGPEPFLLDDAVARLTRALFPGQ
                     TELSLARETLDARAAGAETIVQAALTLPWLGDRRLVVARGADGLTAKSGEALIAYAKA
                     PNPATVLVLVADSSLDGSHWLLRALPPAAVVPAPALAGRALAAWLRAHAQAVGYELRE
                     DAAGLLVELSGDDLTRLVGEVEKAALAGGPENRRVTVAEIRAVVGEHRLRHVFDLTRA
                     LSARDAGAALALLESLLNAGEEPLAVLGMLTREARATWQAADALRAGRAPEEIARSLR
                     RPPAAATALLERARSVSVAAAARQLARCWDVERRLKLGGAARPELSLLVADLCAV"
     gene            complement(1308..1829)
                     /locus_tag="VFX87_12240"
     CDS             complement(1308..1829)
                     /locus_tag="VFX87_12240"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEX4995740.1"
                     /translation="MADPARLVRRALALALTSVLLAGCGYSTRGSLPDHIKTVAVPIF
                     KNRTLEPGVESAITSGVVNAFSSGGRVRVVPLTEADAILEGEVVGYTLEGLAFDRSAI
                     VHAYRLRLTLNVEFRDVKRGAMLWRQEGLQETSDFQVQGTVSDTVARGQGAVLQAASE
                     IGRKVVNLAVDRF"
     gene            complement(1822..4386)
                     /gene="leuS"
                     /locus_tag="VFX87_12245"
     CDS             complement(1822..4386)
                     /gene="leuS"
                     /locus_tag="VFX87_12245"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX4995741.1"
                     /translation="MALDERQDRYPFPEVEARWQKAWADRKQFKVSEEPGRPKYYCLE
                     MFPYPSGRIHMGHVRVYAIGDLLARYKWMRGFNVLHPMGWDAFGLPAENAAIDNGVHP
                     AVWTYENIDNMRTQLKRMGISYDWDREVATCDPAYYKWEQLVFIRMLERGLAYRRRSR
                     VNWCPSCQTVLANEQVEAGRCWRCDSEVTIRDIDGWFFKITDYAQELLDWCDRLPGWP
                     ERVLTMQRNWIGRSEGAEFALPVAGRPELRLPIYTTRPDTSFGMTYAVLAPEHPLVDA
                     LAADAEERRRIDAFRAEVGRETEIERLATDRPKRGLRLAARAVNPFTRQEIPLFLADY
                     VLMGYGTGAIMAVPGEDQRDWDFAKAHGLPIVATVKRPEGWQEDQAYTGDGVKINSGF
                     LDGLSIEEAKRKAIDWLVAQGLGQAKINFRLRDWGISRQRYWGAPIPVLYCEKDGMVP
                     EREENLPVLLPRDVQISGKGGSPLAEVSSFVNAVCPRCGGKARRDTDTMDTFVESSWY
                     FLRYCSPTYDRGMFEPDAAAYWMPVDQYIGGIEHAVLHLLYARFYTKALRDLGMLKVD
                     EPFRALLSQGMVIKDGAKMSKSKGNVVDPDDLIRRFGADTARLFSLFAAPPEKDLDWN
                     DRGVEGASRFLNRIWRFVHAHLAELNGAVAGRGAAEGEPAVVGRGAAEATGFARPALS
                     DIGRAFRRVIHGTIQKVTHDIEHDFHFNTAISAVMELVNALHDFERASLDGIARQERS
                     ALLREAVEATLLLLGPVSPHITEELWAALGHRESLFKRSWPVADAAALARDEVEIVVQ
                     VDGRVRGRLTAAVGAQEAEIREMALADGKVLPWLGGRQVAKVVVVPGRLVNIVTRG"
     gene            complement(4475..4723)
                     /locus_tag="VFX87_12250"
     CDS             complement(4475..4723)
                     /locus_tag="VFX87_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019296115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995742.1"
                     /translation="MKMLMMAIAIVALAASPVLANQCPLLIKQVQDATAGKTDDASKK
                     ALALAAEAKALHDAGKHAESVAKADEAAKAINLTLKKK"
     gene            complement(4762..6594)
                     /gene="uvrC"
                     /locus_tag="VFX87_12255"
     CDS             complement(4762..6594)
                     /gene="uvrC"
                     /locus_tag="VFX87_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX4995743.1"
                     /translation="MTLEEKIDQVPDRPGVYLYKDTKAQIIYIGKAASLRSRVRSYFQ
                     ESRARDPKTDALVGHIRDLEYIVTANELEAMILESTLVKKHRPRYNIILRDDKHYPFL
                     KLTTNEEFPRLVVARRVQKDGATYFGPFYPATAMRETLRLVRQLFPLRTCRIKIDGTL
                     SRPCLQYYIHRCNAPCTGWETAEGYARTVRDVERFLEGKNDDLAARLTREMETAAEET
                     NFERAAALRDRIQALNTVRERQQMISTEEEDQDVVGVVRQGAEACVQLFFVRKGRLLG
                     RESFFFERVAGSSDGEILSAFIRQFYAKNVVPPPELLLSETPPEIDLTGQWLAQRRGG
                     RVDLLAPQRGRKRDLVAMAEENAALALQTHLLARGSRQQVVLEDLERSLALPGPPHRI
                     ECFDISTHQGKETVASMVVWQDGDMKKDDYKRFKIRTVVGTDDFASMQEVLTRRYGRA
                     LESLGVLPDLILLDGGRGQLGAGMKALEDLGLDYIPLAALAKRAEEVYTPDRLQPLVL
                     DMGSPALQALQRIRDEAHRFAITYHRKLRQRRAIASVLDQIPGVGPTLRTSLLKTLGS
                     AKGVREASVADLASVPKITPKLAQRIYDFFHPSPAGEPSESQGG"
     gene            complement(6758..6967)
                     /locus_tag="VFX87_12260"
     CDS             complement(6758..6967)
                     /locus_tag="VFX87_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995744.1"
                     /translation="MVFKWALIVFAVAAVVLFVLTKVIVFLNPPRPGRPAPLMLGRVQ
                     RALTWTVVVPAALALVLLAVTLLRQ"
     gene            complement(6972..9005)
                     /gene="uvrB"
                     /locus_tag="VFX87_12265"
     CDS             complement(6972..9005)
                     /gene="uvrB"
                     /locus_tag="VFX87_12265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX4995745.1"
                     /translation="MSKPLFTLASEFSPSGDQPQAIERLVSVIGAGSPHTVLLGVTGS
                     GKTFTLANVITRLSRPALVISPNKTLAAQLYSEFKAFFPKNAVEYFVSYYDYYQPEAY
                     IPQSDTYIEKDALVNDEIDRMRHSATTSLFERRDVVVVASVSCIYGIGEPETYHGMHV
                     SLRPGQTMDRDALIRALVAVQYERNDYDFRRGTFRVRGDVVEVFPAAAESEALRVEFW
                     GDSVERLMTIDPLRGVTTGAVGDARIYPASHYVTPAAQLERAIEGIMDELRERLGFLK
                     ARGRLLEAQRLEQRTLFDMEMLRELGYCHGVENYSRHLSGRKPGQNPPTLMDYLPKDA
                     LVILDESHVTAPQLRGMYPGDRSRKETLVEYGFRLPSAFDNRPLTFPEFIRLVPQTLY
                     VSATPGPYELGLAGVERGAPGSPLTGGTVAEQIIRPTGLMDPKITVRPASSQVDDLMA
                     EVRARAERDERVLITTLTKRMAEDLTEYYQQNGLRVRYLHSDIDTLERVAVIRDLRLG
                     KFDGLIGINLLREGLDLPEVSLVAILDADKEGYLRSATSLIQTSGRAARHVNGEVIMY
                     ADTVTGSMKAALAETERRRVVQGEYNRAHGITPESVRRTIRDLLESVPERDYYTVEVE
                     RAPQTRWESPAALAAHVKELEGSMKDAARRLDFERAAELRDRIKALRKVDLGV"
     gene            complement(9028..9558)
                     /locus_tag="VFX87_12270"
     CDS             complement(9028..9558)
                     /locus_tag="VFX87_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX4995746.1"
                     /translation="MDEIAVLVLNYTYEPLHFTNGRRAITLLLAGKAESVEASPRVIR
                     SPSRSFHLPAVIRLGAYIRKPFLERVAFNKKNILRRDGYTCQYCSRRGEKLTVDHVVP
                     RSRGGQTTWTNVVAACLRCNLLKGNRMLEEARLRLIREPVHPQFLFSVHLLRHPHATS
                     FLDSWRKYLVAVPSTP"
     gene            complement(9609..10025)
                     /locus_tag="VFX87_12275"
     CDS             complement(9609..10025)
                     /locus_tag="VFX87_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995747.1"
                     /translation="MIVRRWLEAMLALVSLAILYFTHRPEALPRALDLSNDTGLSLWD
                     WIFRGMAFGLLGVWGFSAIVVGFFLLYSPIYVINKAPHLVGKGGWLDRREVRFYLACF
                     ALVCLLVILLTRSFDAAGVVFVILAGFGPVVWRLLV"
     gene            complement(10105..11115)
                     /locus_tag="VFX87_12280"
     CDS             complement(10105..11115)
                     /locus_tag="VFX87_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX4995748.1"
                     /translation="MTRPSARTLSVIDYHTEGEPMRFIVDGAPRIPGASLMEQSEHLA
                     AHGRDLLGMALYEPRGHAAMCGAILTDPVTPGADVGVLFIEPLGPVHMCGHGAIALGA
                     MLVETGRLPAPGGVATVTLDTPAGPVSCRVVSEPGRPTSVTIRNVPAFSVGLDRHVEV
                     PGLGRVAFDLAYGGHFYALVEAAACGLTLEPKEAARLVDVGERIRLAIEARVPLVHPA
                     MPAARGLLYVQFHEPARRADAQLRNAVVVAPAGLDRSPCGTGTSARLANLHARGRLRV
                     GEAFGHESIIGTLFTGRIAALTHVGGTPAVVPEITGRAWMTARATLSLDPTDPFPGGF
                     LL"
     gene            complement(11112..12062)
                     /locus_tag="VFX87_12285"
     CDS             complement(11112..12062)
                     /locus_tag="VFX87_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX4995749.1"
                     /translation="MTSARERPRRLATVDLGTNTVRLLVVEANDGGWRALHQTQRVTR
                     LGEGQAAAGRLLPEPMRRTAAAVAEFAHAARGLGATRVRIVATSAVREARNRAEFVAR
                     LEAESGESVEVVSGEDEARLTLQGVTSGLPGLGASFVLFDIGGGSTEFVLARAGGAAA
                     AVSLRLGVVPLVEEWGEPGPVRWDRFARLRETIEQRLAAEVPDSIAAAGAGELVGTAG
                     TVTTLAALDLGLPAYDAARVHGHRLGRAAVERELARLGALSVADRGRVPCLEPGRADV
                     IIPGIAICLAAMARFRSEHLVVSDRGLREGILCELLGAAR"
     gene            complement(12059..14326)
                     /locus_tag="VFX87_12290"
     CDS             complement(12059..14326)
                     /locus_tag="VFX87_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4995750.1"
                     /translation="MTGDSVRAALSQLGDVLDNLRERLGDGPSRELHPDRDAHALDIF
                     QEILSVPPLGLPPGELFGLAMDRVSRLVAADRALLFVLDDATGRLVPRSGRGFRRDDL
                     ESVVLDPGEGLVGRVFAEKRVLTYDAAAAPPARDPFVERFPMRQGIAVPVRTEGEVGG
                     VLFAGRQDLGAPFTTTDVLLLLVIADRVGSSLVHQRLLERRGDHIAHLRELRGLVDAN
                     LPAREPREILARASDAACRLAGVRGALALAGAAPGRIGVLAGAGLLASVGPERLRADD
                     DVLVDGFAAEGPVTIRDLQARRPGRPGVFEEEGIRAALLVPMRARGRVVGLLCLADPE
                     PRDFSPEETESALMLAALTAGALETERQIAESRHAAADRDAEHAGLRREERTRVLATL
                     GAGLTRELHPVFATLLGRAQLLLARAPNDPLREGLVALEEAAWRGTDLLQRLLGLAEA
                     DQRGGVADLPAIAQEAVSFARASLRAEAESRGAIEMTADLGATPPVEASAVALREAVV
                     GLVLNAVEAMPAGGTLSVRTRGHEGGAELVLVDTGEGIPPEIRPRIFDPFFTTRPGHL
                     GLGLSVAEAVVLRAGGRLEVDRAGAGGGTRVTVWLPAAGVRPAAPPPEAAEPARPTAT
                     EARPPAPLTGSVLVVEEEEGIRTEVLDTLMAVGHRVEAVPDADAALARLGQGGIDVVL
                     TDLALRDRSGLHLASAVKKRSPGTAVVLLTGWGRRLHEERLRESGVDVMLVKPVQPER
                     VREAVADALTLRRPA"
     gene            complement(14356..14787)
                     /locus_tag="VFX87_12295"
     CDS             complement(14356..14787)
                     /locus_tag="VFX87_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4995751.1"
                     /translation="MTSFSLATRAGGLALAVVRLVLAPGRGPGDVAAQTSQPWAAPAS
                     EKAKKNPMPADKKSVEQGEKVAKVNCVSCHGSHGKGNGPAAVALNPKPADWTSKRVQD
                     EQDGEIFWKITTGRGAMPSWRHLPENDRWALVRYIRTLAGK"
BASE COUNT         2150 a         5378 c         5268 g         2050 t
ORIGIN      
        1 cagaggaggc gcacgccgcc cccctcgagc acgagggcgc ggaccttgat cggatcgtgg
       61 accccggtcg aggggcggaa ccagaaggtg tcggggaagc ggccgatgag atcggggatc
      121 caggcgcggc gcgggaaccc cccgtagccg gcgagcgggg tgtccccggg cagctcgacg
      181 gtcaccgcgg ccgcgccggc gagcaggcgc ggcgcggggg cggcggccac cggccccgcc
      241 cccgatgcca gcagcagcgt ggcggcgacg agcgcgcgcc gcgcgcccgc catcacaccg
      301 cgcacagatc ggcgacgagc agcgacagct cgggacgcgc ggctcccccc agcttcagac
      361 gtcgctccac gtcccagcag cgggcgagct gtcgcgcggc cgccgcgacg gacacgctcc
      421 gcgcccgctc gagcagggcg gtggccgcgg cgggcggccg ccgcaggctc cgcgcgatct
      481 cctcgggcgc gcggccggcc cggagcgcgt ccgcggcctg ccacgtcgcg cgggcctcgc
      541 gggtcagcat gccgagcacc gcgagcggct cttcccccgc gttcagcagc gactcgagga
      601 gcgccagcgc ggctccggcg tcgcgcgccg acagggcgcg ggtgaggtcg aagacgtggc
      661 gcagccggtg ctcgccgacc accgcgcgga tctcggccac cgtcacgcgc cggttctccg
      721 ggccgcccgc gagcgccgcc ttctccacct cgcccaccag gcgggtgaga tcgtcgcccg
      781 agagctccac cagcaggccg gccgcgtcct cgcgcagctc gtagccgacg gcctgggcgt
      841 gggcgcggag ccacgccgcg agcgcgcggc ccgcgagcgc cggggccggc accaccgcgg
      901 cgggcggcag cgcgcgcagc agccagtgcg agccgtccag cgacgagtcg gccacgagca
      961 ccagcacggt ggccgggttg ggggccttcg cgtaggcgat gagcgcctcg ccggacttcg
     1021 cggtcaggcc gtccgcgccg cgcgccacca cgagccggcg gtcgcccagc cacggcaggg
     1081 tgagggccgc ctgcacgatg gtctccgcgc ccgccgcgcg cgcgtcgagg gtctcccgcg
     1141 cgagggagag ctcggtctgg ccggggaaga gcgcgcgggt gaggcgcgcc accgcgtcgt
     1201 cgaggagaaa cggctcgggc ccgtgcagca aggtcaccgg ccccacctgc cccgtgtccg
     1261 cggctcggac gaaacccgcg tagtccaccg ctacgcccgc gcggacacta gaaccggtcc
     1321 accgcgaggt tgacgacctt gcgcccgatc tcgctcgcgg cctggagcac ggccccctgc
     1381 ccgcgggcca ccgtgtccga caccgtgccc tgcacctgga agtccgacgt ctcctgcagc
     1441 ccctcctgcc gccagagcat cgcgccccgc ttcacgtcgc ggaactccac gttcagggtg
     1501 agccgcagcc ggtaggcgtg cacgatggcg ctccggtcga aggcgaggcc ctcgagcgta
     1561 tagcccacca cctcaccctc gaggatggcg tcggcctccg tgagcggcac cacccgcacc
     1621 cggccgccgc tggagaaggc gttgaccacg ccggaggtga tcgcgctctc cacgcccggc
     1681 tcgagcgtgc ggttcttgaa gatgggcacc gccaccgtct tgatgtgatc gggcaggctg
     1741 ccgcgcgtgc tgtagccgca gccggccagc aggacggagg tgagggccag cgccagggcc
     1801 cgtcggacga ggcgcgccgg atcagccacg ggtcacgatg ttgacgagac gcccgggcac
     1861 cacgaccacc ttggcgacct ggcgcccgcc gagccacggg agcaccttcc cgtcggcgag
     1921 ggccatctcg cggatctccg cctcctgggc gcccaccgcc gcggtgaggc ggccccgcac
     1981 gcgcccgtcc acctggacca cgatctccac ctcgtcgcgg gcgagcgcgg ccgcatccgc
     2041 gacgggccac gaccgcttga acaggctctc gcgatgtccg agcgcggccc acagctcctc
     2101 ggtgatgtgc ggcgacaccg gcccgagcag gagcagcgtg gcctccaccg cctcccgcag
     2161 cagcgcgctc cgctcctgcc gcgcgatccc gtccagcgat gcccgctcga aatcgtggag
     2221 cgcgttcacg agctccatca ccgcgctgat cgcggtgttg aagtggaagt cgtgctcgat
     2281 atcgtgcgtc accttctgga tcgtcccgtg gatcacgcgc cggaaggcgc ggccgatgtc
     2341 ggacagtgcg ggtcgcgcga agccggtagc ttcggctgca cctcgcccta ccactgccgg
     2401 ctcgccttcg gctgcgcctc gcccagcaac tgccccattc agttcggcga ggtgagcgtg
     2461 gacgaagcgc cagatgcggt tgaggaagcg cgaggcgccc tcgacaccgc ggtcgttcca
     2521 gtcgaggtcc ttctcgggcg gggccgcgaa gagcgagaag agccgcgcgg tgtcggcccc
     2581 gaagcggcga atgagatcat ccgggtccac cacgtttccc ttggacttcg acatcttggc
     2641 cccgtccttg atgaccatcc cctgtgagag gagggcccgg aagggctcgt ccaccttcag
     2701 catcccgagg tcgcgcagcg ccttggtgta gaagcgcgcg tagaggagat ggagcaccgc
     2761 gtgctcgatg ccgccgatgt actggtccac cggcatccag taggcggcgg cgtccggctc
     2821 gaacatgccg cggtcgtagg tcggcgagca gtagcggagg aagtaccagg acgactccac
     2881 gaaggtgtcc atggtgtcgg tgtcccgccg cgccttgccc ccgcaccggg gacagaccgc
     2941 gttgacgaag ctcgacacct ccgcgagcgg tgacccgccc ttgccgctga tctgcacgtc
     3001 gcgcggcagc agcaccggca ggttctcctc ccgctccggc accatgccgt ccttctcgca
     3061 gtagagcacc gggatcggcg ccccccagta gcgctgccgg ctgatccccc agtcgcggag
     3121 gcggaagttg atcttggcct ggccgagccc ctgcgccacg agccagtcga tcgccttgcg
     3181 cttggcctcc tcgatcgaga gcccgtcgag gaagcccgag ttgatcttca cgccgtcgcc
     3241 ggtataggcc tgatcctcct gccagccctc cgggcgcttc acggtcgcga cgatggggag
     3301 cccgtgtgcc ttggcaaaat cccagtcgcg ctggtcctca cccggcaccg ccatgatcgc
     3361 gccggtgccg tagcccatca gcacgtagtc ggcgaggaag agcgggatct cctggcgggt
     3421 gaacgggttc accgcgcggg ccgcgaggcg caggccgcgc ttcggccggt cggtagcgag
     3481 ccgctcgatc tcggtctcgc gcccgacctc ggcgcggaaa gcgtcgatcc ggcggcgctc
     3541 ctccgcgtcc gccgcgaggg cgtccaccag cgggtgctcg ggggccagca ccgcgtaggt
     3601 catgccgaag gaggtgtcgg ggcgcgtggt gtagatcggg agcctgagct ccggccgtcc
     3661 cgccaccggc agcgcgaact cggcgccctc actgcggccg atccagttgc gctgcatggt
     3721 gagcacccgc tccggccagc cgggcagccg gtcgcaccag tcgagcagct cctgggcgta
     3781 gtcggtgatc ttgaagaacc agccgtcgat gtcgcggatc gtcacctcgg aatcgcagcg
     3841 ccagcagcga cccgcctcga cctgctcgtt ggccaggacg gtctggcagg acgggcacca
     3901 gttcacgcgc gaccggcgcc ggtaggcgag gccgcgctcg agcatccgga tgaagacgag
     3961 ctgctcccac ttgtaatagg cgggatcgca ggtggccacc tcgcgatccc agtcgtagga
     4021 gatgcccatg cgcttgagct gcgtgcgcat gttgtcgatg ttctcgtagg tccacaccgc
     4081 ggggtgcacc ccgttgtcga tggccgcgtt ctcggcgggc aggccgaagg cgtcccagcc
     4141 catcgggtgc agcacgttga agccccgcat ccacttgtag cgggccagca gatcgccgat
     4201 cgcgtagacc cgcacgtggc ccatgtggat gcggccggac ggatacggga acatctcgag
     4261 gcagtagtac ttgggccggc cgggctcttc gctcaccttg aactgcttgc ggtccgccca
     4321 ggccttctgc cacctggcct cgacctccgg gaaggggtag cggtcctgac gctcgtcgag
     4381 ggccatattg gccctgattt ttacacgctt aggcgggggg cgcaagaaac ggctcccggg
     4441 ggcctggagc ggcccccggg agcacggacc gggcttactt cttcttcagg gtgaggttga
     4501 tcgccttcgc ggcctcgtcc gccttcgcta ccgactcggc gtgcttgccg gcgtcgtgga
     4561 gcgccttggc ctcggcggcc agggccaacg ccttcttgga tgcgtcgtcg gtcttgcccg
     4621 ccgtcgcgtc ctggacctgc ttgatcagga gcgggcactg gttggccagc accggcgaag
     4681 cggccagcgc gacaatcgcg atcgccatca tgagcatctt catgcgcgtc accccctttc
     4741 ggtgtagtcc tactaggacg tttaaccccc ctggctctcg gaaggttccc ccgccgggct
     4801 cgggtggaag aaatcataga tccgttgcgc cagcttcggg gtgattttcg gcaccgaggc
     4861 caggtcggcc accgaggcct cccgcactcc cttcgcggag cccagggtct tcagcaggct
     4921 cgtgcggagg gtcggcccca ccccggggat ctggtccagc acggaggcga tggcccggcg
     4981 ctgacgcagc ttccggtggt aggtgatcgc gaagcgatgc gcctcgtcac ggatcctctg
     5041 gagcgcctgc aacgcgggcg agcccatatc cagcacgagg ggctggagac ggtccggggt
     5101 gtagacctcc tcggcgcgct tggcgagcgc ggcgagcggg atgtagtcga ggcccaggtc
     5161 ctcgagcgcc ttcatccccg cgccgagctg gccgcggccg ccgtcgagca ggatgaggtc
     5221 gggcagcacg cctaggctct ccagcgcgcg gccgtagcgg cgggtcaaca cctcctgcat
     5281 cgacgcgaag tcgtcggtgc ccacgacggt ccggatcttg aagcgcttgt agtcgtcctt
     5341 cttcatgtcg ccgtcctgcc acaccaccat ggaggcgacg gtctccttgc cctggtgcgt
     5401 cgagatgtcg aagcactcga tgcgatgcgg cggtccgggc agcgcgagcg agcgctcgag
     5461 atcctccagc accacctgct ggcggctgcc gcgcgcgagc aagtgggtct ggagcgcgag
     5521 cgccgcgttc tcctcggcca tcgccacaag gtcgcgcttg cgtccccgct gcggggccag
     5581 caggtcgacg cgcccgcccc ggcgctgggc cagccattgc ccggtcagat cgatctcggg
     5641 cggcgtctcc gagagcagga gctcgggagg cgggaccacg ttcttcgcgt agaactggcg
     5701 gatgaacgcg gagaggatct cgccgtcgct cgagccggcc acccgctcga agaagaacga
     5761 ctcccgcccg agcagccgcc ccttgcgcac gaagaagagc tgcacgcagg cctcggcgcc
     5821 ctggcggacc acccccacca cgtcctgatc ctcctcctcg gtggagatca tctgctgccg
     5881 ctcccgcacc gtgttgagcg cctggatgcg gtcgcgcagg gcggcggccc gctcgaagtt
     5941 ggtctcctcg gccgcggtct ccatttcccg cgtgagccgg gccgcgaggt cgtcgttctt
     6001 gccctcgaga aagcgctcca cgtcccggac cgtccgcgcg tagccttccg cggtctccca
     6061 gcccgtacag ggggcattgc agcgatggat gtagtactgc aggcacgggc gcgagagcgt
     6121 cccgtcgatc ttgatccggc acgtgcgcag agggaagagc tgccgcacca atcgtaacgt
     6181 ttcccgcatc gcggtggccg ggtagaacgg gccgaagtag gtcgccccgt ccttctgcac
     6241 ccgccgggcc accaccaggc gcgggaactc ctcgttggtg gtgagcttga ggaagggata
     6301 gtgcttgtcg tcccgcagga tgatgttgta gcgggggcgg tgcttcttga cgagcgtgga
     6361 ctcgagaatc atcgcctcga gctcgttcgc ggtgacgatg tactcgagat cgcggatgtg
     6421 gccgaccagc gcgtcggtct tcgggtcgcg cgcccgcgac tcctggaagt aggaccgcac
     6481 gcggctgcgg agcgacgcgg ccttgccgat gtagatgatc tgggccttgg tgtccttgta
     6541 gaggtagacg cccggccggt cgggcacctg gtcgatcttc tcctcgagcg tcattcagtt
     6601 gacatccctg acctcatttt cgaatgggct cggctcgggt tcttggatcc actcgactcg
     6661 ctccggcttc gtctcgccga ccgggctgcg cctcgccacc ccctgacttc attttcgaat
     6721 gcgctcggct cgccttcggc tgcgcctcgc caagcagtca ctgccggagc agggtcaccg
     6781 cgagcaggac cagggccagc gccgccggca ccaccacggt ccacgtgagc gcccgctgga
     6841 cgcggcccag catgagcgga gcgggccggc ccggtcgcgg cgggttcagg aacacgatga
     6901 ccttcgtgag cacgaacagc accaccgcgg ccaccgcgaa gacgatcagg gcccacttga
     6961 ataccattcg ctcagacgcc caggtcgacc ttgcggaggg ccttgatgcg gtcgcgcagc
     7021 tcggcggccc gctcgaagtc gagccggcgc gccgcgtcct tcatggaccc ctccagctcc
     7081 ttcacgtggg ccgcgagggc ggcggggctc tcccaccgcg tctggggggc gcgctccacc
     7141 tccaccgtgt agtagtcccg ctcgggcacc gattcgagca ggtcccggat cgtgcggcgc
     7201 accgactccg gggtgatccc gtgggcgcgg ttgtactcac cctgcaccac gcgccggcgc
     7261 tcggtctcgg cgagggccgc cttcatcgag ccggtcacgg tgtccgcgta catgatcacc
     7321 tcgccgttga cgtggcgcgc cgcgcggccc gaggtctgga tcagcgacgt ggcggagcgc
     7381 aggtagccct ccttgtcggc gtccaggatc gcgaccagcg agacctccgg caggtccagc
     7441 ccctcccgca agaggttgat cccgatgagc ccgtcgaact tgccgagccg gaggtctcgg
     7501 atgaccgcca cccgctcgag cgtgtcgatg tcggaatgca ggtagcggac gcggagaccg
     7561 ttctgctggt agtactcggt gagatcctcg gccatgcgct tggtgagcgt ggtgatgagc
     7621 acccgctcgt cgcgctccgc ccgggctcgc acctccgcca tgagatcgtc cacttgggag
     7681 gacgcgggac gcaccgtgat cttcgggtcc atgaggccgg tgggccggat gatctgctcc
     7741 gccaccgtcc cgcccgtgag tgggctgccc ggcgctcctc gctcgacccc ggccagcccg
     7801 agctcatagg ggcccggggt cgcggagacg tagagggtct gcggcaccag gcggatgaac
     7861 tccgggaagg tgagcggacg attgtcgaag gccgatggta gccggaagcc gtactcgacc
     7921 agcgtctcct tgcgcgagcg gtcgcccggg tacatgccgc ggagctgggg tgcggtcacg
     7981 tgcgactcgt cgaggatgac cagcgcgtcc ttcgggaggt agtccatcag ggtcggcgga
     8041 ttctgccctg gcttgcgccc ggagagatgc cgcgagtagt tctcgacccc gtggcagtag
     8101 ccgagctcgc gcagcatctc catgtcgaag agcgtgcgct gctcgaggcg ctgcgcctcc
     8161 agcagccggc cgcgcgcctt gaggaacccg agccgctcgc gcagctcgtc catgatgccc
     8221 tcgatcgcgc gctcgagctg ggcggccggg gtcacgtagt ggctcgccgg gtagatccgc
     8281 gcgtcaccca ccgcgccggt ggtgacccca cggagggggt cgatggtcat gagccgctcg
     8341 acgctgtcgc cccagaactc cacccgcagc gcctccgatt ccgcggcggc cgggaacacc
     8401 tcgaccacgt cgccccgcac ccggaaggtg ccgcggcgga aatcgtagtc gttgcgctcg
     8461 tactgcaccg ccaccagggc ccggatgagg gcgtcccggt ccatggtctg gcccgggcgc
     8521 agcgacacgt gcatgccgtg gtaggtctcg ggctcgccga tgccgtagat gcaggacacc
     8581 gaggcgacca cgaccacgtc gcggcgctcg aagagcgacg tggtcgccga gtgccgcatg
     8641 cggtcgatct cgtcgttcac cagcgcgtcc ttctcgatgt aggtgtccga ctgcgggatg
     8701 taggcctccg gctggtagta gtcgtagtag gagacgaagt actcgaccgc gttcttcggg
     8761 aagaacgcct tgaactcgct gtacagctga gcggccagcg tcttgttcgg cgagatcacc
     8821 agcgcgggcc ggctgagccg cgtgatcacg ttggcgagcg tgaaggtctt gccgctgccg
     8881 gtgactccca gcagcacggt gtgggggctc ccggccccga tcaccgacac caggcgttcg
     8941 atcgcctgcg gctggtcccc ggacgggctg aactccgagg cgagcgtgaa caacggcttg
     9001 gacaactggt ggctcagggg gagcggctca gggcgtcgag ggcaccgcca ccaggtactt
     9061 tcgccacgag tcgaggaacg aggtcgcgtg cggatgccgg agcaggtgca ccgagaagag
     9121 gaactggggg tgcacgggct cccggatcag ccgcagtcgg gcctcctcca gcatgcggtt
     9181 gcccttgagc aggttgcagc gcaggcaggc ggccaccacg ttggtccagg tggtctggcc
     9241 ccctcgcgag cgcgggacca cgtgatccac cgtgagcttc tcgccgcgtc gcgagcagta
     9301 ctggcaggtg tagccgtccc gccgcaggat gttcttcttg ttgaaggcca cccgctccag
     9361 gaacggcttc cggatatagg ccccgaggcg gatgaccgcg gggaggtgga agctccggga
     9421 gggggaccgg atcacgcggg gggaggcctc gacgctctcg gccttccccg ccaagagcag
     9481 cgtgatggct cgccgaccgt tggtgaaatg cagcggctcg taggtgtaat tcagcaccag
     9541 gaccgcgatt tcatccatat acccggcaag gatagacaag gcgccggggc ctgtcaaccg
     9601 cggcgaggct agaccaggag ccgccagacc accgggccga acccggcgag gatcacgaac
     9661 accacgcccg ccgcgtcgaa cgaccgtgtc agcaggatga cgagcaggca cacgagcgcg
     9721 aagcacgcga ggtagaagcg gacttcacgg cggtcgagcc acccaccctt gccgacgaga
     9781 tgtggcgcct tgttgatgac atagatcggc gagtacagca ggaagaatcc caccacgatc
     9841 gccgagaacc cccagacgcc aagcaggccg aacgccatgc cccggaagat ccagtcccac
     9901 agggacaacc cggtgtcgtt actgaggtcg agcgcgcggg gtagcgcttc cgggcgatgc
     9961 gtgaagtaca ggatggccag ggagacgagc gccagcatcg cctcgagcca ccgccgaacg
    10021 atcacccgag ggatgaaact catccaggct aggacaacac aggagccgcc gacgtgtcaa
    10081 taaatgggcc cgagcggcgc gcggtcagag caggaagccg ccgggaaagg ggtcggtggg
    10141 gtcgagggac agcgtggcac gggcggtcat ccaggcccgt ccggtgatct ccgggaccac
    10201 cgccggggtg ccccccacat gggtgagcgc ggcgatccgc ccggtgaaga gcgtgccgat
    10261 gatggactcg tggccgaagg cctcccccac ccggagccgg ccgcgcgcgt ggaggttggc
    10321 gagtcgcgcg gaggtcccgg tcccgcaggg cgagcggtcg aggcccgcgg gcgccaccac
    10381 caccgcgttg cgcagctgcg cgtcggcccg ccgcgccggc tcgtggaact gcacgtagag
    10441 cagcccgcgc gccgcgggca tcgcggggtg caccagcggc acgcgcgcct cgatggcgag
    10501 gcggatccgc tcgcccacgt ccacgaggcg ggccgcctcc ttcggctcga gcgtgagacc
    10561 gcaggccgcc gcctccacca gcgcgtagaa gtggccgccg taggccaggt cgaacgccac
    10621 acggccgagc ccgggcacct ccacgtggcg gtcgagcccc accgagaacg cggggacgtt
    10681 gcggatcgtg accgaggtcg gccgccccgg ctcgctcacc acgcggcagg agaccggccc
    10741 ggcgggcgtg tcgagcgtca cggtggcgac gccgcccggc gcgggcagcc ggcccgtctc
    10801 caccagcatg gccccgagcg cgatggctcc gtgcccacac atgtgcaccg ggccgagcgg
    10861 ctcgatgaag agcacgccga cgtcggctcc cggcgtcacc ggatcggtca ggatcgcgcc
    10921 acacatcgca gcatggccgc gcggctcgta gagcgccatg cccagcaggt cgcggccgtg
    10981 cgcggccagg tgctcgctct gctccatcag cgaggcgccg gggatccgag gcgcaccgtc
    11041 cacgatgaac cgcatgggct cgccctcggt gtggtagtcg atcaccgaca gcgtgcgcgc
    11101 cgacggtctc gtcaccgcgc cgctccgagc agctcgcaga gaatgccttc gcggagcccg
    11161 cgatcgctca ccaccaggtg ctcgctccgg aagcgcgcca tcgcggccag gcagatcgcg
    11221 atgccgggaa tgatcacgtc cgcgcgcccc ggctcgaggc acggcacccg tccgcggtcg
    11281 gcgacggaca gcgcgccgag ccgggccagc tcccgctcca ccgccgcgcg gccgagccgg
    11341 tggccgtgaa cccgcgccgc gtcgtacgcg ggcaggccga gatcgagcgc ggccagcgtg
    11401 gtgacggtgc ccgcggtgcc gaccagctcg cccgcccccg ccgccgcgat gctatccggc
    11461 acctcggcgg ccaggcgctg ctcgatcgtc tcgcgcaggc gcgcgaagcg atcccaccgc
    11521 accggcccgg gctcgcccca ctcttccacg agcgggacca cgcccagccg caagctcacc
    11581 gcggccgcgg cgccgccggc gcgggcgagc acgaactcgg tgctgcctcc accgatgtcg
    11641 aagagaacga acgaggcgcc gaggccgggc agcccggagg tgacaccctg cagcgtgagg
    11701 cgcgcctcgt cctcgccgga gacgacctcc acggactcgc ccgattccgc ctccaggcgc
    11761 gccacgaact ccgcgcggtt gcgcgcctcg cgcaccgcgc tcgtggccac gatccggacg
    11821 cgggtggcgc cgaggccgcg cgcggcgtgc gcgaactcgg cgaccgcggc ggcggtgcgc
    11881 cgcatcggct ccgggagcag ccgaccggcg gccgcctgcc cttccccgag ccgggtgacc
    11941 cgctgcgtct gatggagcgc gcgccagccg ccgtcgttcg cttcgacgac gaggagacgg
    12001 acggtattgg tgccgagatc gacggtcgcg agacgccgcg gccgctcgcg tgcggaggtc
    12061 acgccgggcg gcggagggtg agcgcgtccg cgaccgcttc gcggacccgc tccggctgca
    12121 cgggcttcac cagcatgacg tccacaccgg attcgcggag ccgctcctcg tggagccggc
    12181 ggccccatcc ggtgagcagc accaccgccg ttccgggact ccgcttcttg accgcggaag
    12241 cgagatgaag tcccgatcgg tcgcgcaggg ccaggtcggt gagcaccacg tcgatgccgc
    12301 cctgcccgag ccgcgccagc gcggcgtccg cgtccggcac cgcctccacg cgatgtccca
    12361 ccgccatgag ggtgtccagc acctcggtgc gaatcccttc ctcctcctcg accacgagga
    12421 cggagccggt gagcggagcc ggcggacggg cctcggtcgc ggtggggcgg gcgggctccg
    12481 ccgcttcggg cgggggcgcg gccggccgca cccccgccgc gggcagccac acggtcaccc
    12541 tcgtgccgcc gccggcgccg gcgcggtcca cctcgaggcg cccgcccgcg cggagcacca
    12601 ccgcctccgc cacgctgagg ccgagcccca ggtggccggg gcgcgtggtg aagaagggat
    12661 cgaagatccg cggacggatc tcgggcggga tgccctcacc ggtgtcgacc aggaccagct
    12721 cggcgccccc ctcgtggccg cgggtgcgca cgctcagggt cccgcccgcg ggcatcgcct
    12781 ccaccgcgtt cagcacgagg ccgaccaccg cctcgcgcag tgcgacggca ctggcctcga
    12841 ccgggggcgt cgcgcccaga tccgcggtca tctcgatcgc gccccgcgac tcggcctcgg
    12901 cgcggaggct cgcgcgcgcg aagctcaccg cttcctgcgc gatggccggc aggtccgcga
    12961 ccccgccgcg ctgatcggcc tccgccagcc cgagcaggcg ctggagcaga tcggtgccgc
    13021 gccacgcggc ttcctcgagc gcgaccaggc cttcgcgcag gggatcgttc ggggcgcggg
    13081 cgagcaggag ctgcgcgcga cccagcaggg tcgcgaagac cgggtggagc tcccgcgtga
    13141 gtccggcccc gagcgtcgcg agcacgcggg tccgctcctc gcgccgcagg ccggcgtgct
    13201 ccgcgtcgcg gtcggcagcc gcgtggcggc tctcggcgat ctgccgctcc gtctcgagcg
    13261 ctcccgcggt cagggccgcg agcatcagcg cggactccgt ctcctccggc gagaagtccc
    13321 gcggctccgg gtcggccagg cagaggagcc ccaccacgcg gccgcgcgcg cgcatcggca
    13381 ccagcagggc cgcgcggatt ccctcctcct cgaacacgcc gggccggccg ggccgccggg
    13441 cctggagatc gcggatcgtc accggaccct cggcggcgaa gccgtccacg agcacgtcgt
    13501 cgtccgcgcg caagcgctcg ggcccgacgc tggcgagcag tcccgcgccc gcgagcacgc
    13561 cgatgcgccc gggagccgcg ccggccagcg cgagggcacc gcgcaccccc gccagccggc
    13621 aggccgcatc ggaggcacgg gccaggatct cgcgcggctc ccgcgcgggg aggttcgcgt
    13681 ccacgagccc gcgcagctcc cgcaggtgcg cgatgtgatc gccccggcgc tcgagcaacc
    13741 gctggtggac gagcgaggag ccgacgcggt cggcgatgac gaggagcagg agcacgtcgg
    13801 tggtggtgaa gggcgcgccc agatcctggc ggcccgcgaa gagcacgccg ccgacctcgc
    13861 cctcggtccg caccggcacc gcgatgccct gtcgcatcgg gaaccgctcg acgaacgggt
    13921 cgcgagcggg cggcgcggcg gccgcgtcgt aggtcagcac gcgcttctcg gcgaagacgc
    13981 gcccgaccag cccctcgccc ggatcgagca ccacgctctc caggtcgtcg cgccggaagc
    14041 cgcggcccga gcggggcacg agccgcccgg tcgcgtcgtc caggacgaac agcagcgccc
    14101 ggtccgcggc gaccagccgg gaaacgcgat ccatcgccag cccgaatagc tcgccggggg
    14161 gcagcccgag cggcgggacc gagaggatct cctggaagat gtcgagggcg tgcgcgtccc
    14221 ggtcgggatg gagctcccgg ctcggcccgt cgcccaggcg ctcccggagg ttgtcgagga
    14281 cgtcgcccag ctgcgacagg gcggcgcgga cggagtcgcc ggtcatctag acgcgcctcg
    14341 agctgggcgg cgcggtcact tcccggcgag cgtgcgaatg tatcggacca gggcccagcg
    14401 gtcgttctcg gggaggtgcc gccacgaggg catggccccc cgtccggtgg tgatcttcca
    14461 gaagatctcc ccgtcctgct cgtcctggac ccgcttcgag gtccagtccg ccggcttcgg
    14521 attcagcgcg accgccgcgg gcccgttgcc cttgccgtgg gaaccgtggc aggacacgca
    14581 gttgaccttg gcgaccttct cgccctgctc gaccgacttc ttgtccgcgg gcatcgggtt
    14641 cttcttggcc ttctcggacg cgggggcggc ccagggttgc gatgtctggg cggcgacatc
    14701 ccccgggccg cgccccgggg cgagaaccag ccggaccacg gccagggcca gtccaccggc
    14761 acgggtagcg agcgagaacg acgtcatcga ggggcctcct gtggcaacgg gaacctgaag
    14821 cgaatcgtcc cgtcatcatt gacgaa
//
LOCUS       DASUFA010000333         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1663939, whole genome shotgun sequence.
ACCESSION   DASUFA010000333 DASUFA010000000
VERSION     DASUFA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663939"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFX87_12300"
     CDS             <1..270
                     /locus_tag="VFX87_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX4995752.1"
                     /translation="YGDVAWVRSARAAGQVTLSRGSGSEILSIRELPSAEAAPVLQRY
                     ITRVPITRPYFDAKPDSPLSDFVSEAPRHPVFQLGPGGARRNVGM"
     gene            complement(283..978)
                     /locus_tag="VFX87_12305"
     CDS             complement(283..978)
                     /locus_tag="VFX87_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4995753.1"
                     /translation="MGAAIARLDDVAMDEVHLATPVSDEAIRSLKLGDVVYLSGALYT
                     AREGVYRQVVDKGVAFPGAVRALTNVNFHCSPAAATRPDGTHVVEAVTATASFRFGKS
                     MGPWFERSGARVIVGKAGLTELAYREWFVPHGAVYLTTVGYGMGAAYGRGITRVREVH
                     WLRELGIAQALWVLEVNRLGPFLVEGDAEGRSLFALANREINLRIAEVYKTLPPPALA
                     RFGEVLSRTDEVV"
     gene            complement(978..1916)
                     /locus_tag="VFX87_12310"
     CDS             complement(978..1916)
                     /locus_tag="VFX87_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX4995754.1"
                     /translation="MPNVIPAKLIEDTARELSARAAIDIPVDYREGVRLARDREQNRL
                     ARFVLDQMLGNWEIASADRRPMCADTGLPRYYVRLGNEAAVEGGLVALERALRKATAE
                     ATQAIPLRPNRVHPLTREDRNDNVGLHAPEVTFAVEPHADWIDVTTVHKGGLFGSDYR
                     MLFPGDGVAGIKRFFVDTLIESGKRGLACQPAIVGVGIGGAKDTCVRLGKEAACLRIV
                     GSRNPDPAAAALEDELTALGNYIGIGAMGFAGTSMVVATHVELAYTHTGGMPVGIHQF
                     CLSSRRATARIHPDGRVEFRSDPGWFTDYYRRAGIE"
BASE COUNT          296 a          728 c          691 g          295 t
ORIGIN      
        1 tacggcgacg tcgcctgggt ccgcagcgcc cgcgccgcgg gtcaggtcac gctgagccgc
       61 ggctcaggct ccgagatact ctcgatccgg gagctgcctt ccgccgaagc cgcccccgtc
      121 ctccagcgct acatcacccg agtccctata acccgccctt acttcgacgc caagccagac
      181 tccccactca gcgatttcgt ctctgaagct cctcgccacc ccgtcttcca gctcggccca
      241 ggtggcgcgc gacgaaatgt cggaatgtaa gacctgaccc cgtcagacga cttcgtcggt
      301 gcgggagagg acttcgccga agcgggcgag ggcgggcggg ggaagggtct tgtagacctc
      361 ggcgatgcgg aggttgatct cgcggttggc gagggcgaag aggctccggc cctccgcatc
      421 gccttccacc aggaaagggc cgaggcggtt cacctcgagg acccagagcg cctgggcaat
      481 cccgagctcg cggagccagt ggacctcgcg cacccgcgtg atcccgcgtc cgtaggccgc
      541 ccccatgccg tagcccaccg tggtgaggta caccgcgccg tggggcacga accactcgcg
      601 ataggccagc tcggtgagcc cggccttgcc cacgatgacg cgcgcgccgg agcgctcgaa
      661 ccacgggccc atcgacttgc cgaagcggaa gctcgcggtc gcggtcaccg cctcgaccac
      721 gtgggtgccg tccgggcgcg tggccgcggc gggcgagcag tggaagttga cgttggtcag
      781 cgcgcgcacc gcacccggga acgccacacc cttgtccacc acctggcgat agaccccctc
      841 gcgcgcggtg tagagcgccc cgctgaggta caccacgtcg cccagcttga gcgagcggat
      901 ggcctcgtcc gacaccggcg tggcgaggtg cacctcgtcc atcgccacgt cgtccaggcg
      961 cgcgatcgcg gcgcccatca ctcgatcccc gcgcggcggt agtagtcggt gaaccagccc
     1021 ggatcgctcc ggaactcgac gcgcccgtcc ggatggatgc gggcggtcgc gcggcgcgac
     1081 gagagacaga actggtggat gcccaccggc atgccgccgg tgtgggtgta ggccagctcc
     1141 acgtgcgtgg ccaccaccat ggaggtgccc gcgaagccca tggccccgat cccgatgtag
     1201 ttgccgagcg cggtcagctc gtcctccagc gccgccgccg cgggatcggg attgcggctg
     1261 ccgacgatgc gcaagcacgc cgcttctttg cccagccgca cgcaggtgtc cttggctccg
     1321 ccgatgccca cgcccacgat ggcgggctgg caggccaggc cgcgcttgcc cgactcgatc
     1381 agcgtgtcca cgaagaagcg cttgatgccg gccacgccgt cgccgggaaa cagcatgcgg
     1441 tagtcgctgc cgaagagccc gcccttgtgc acggtggtca cgtcgatcca gtccgcgtgc
     1501 ggctccaccg cgaaggtgac ctcgggggcg tgcaggccca cgttgtcgtt gcggtcctcc
     1561 cgggtgagcg gatggacgcg attgggccgg agcgggatcg cctgggtggc ctccgcggtc
     1621 gccttccgga gcgcgcgctc gagcgccacc agtccgccct cgaccgcggc ctcgttgccg
     1681 agccgcacgt agtagcgcgg cagcccggtg tccgcgcaca tcggccgccg gtcggcgctc
     1741 gcaatctccc agttgcccag catctggtcg agcacgaacc gcgcgagccg attctgctct
     1801 cggtcccgcg ccagccgcac cccctcgcgg tagtcgacag gaatgtcgat ggcggcacgc
     1861 gcgctcagct cccgcgcggt atcctcgatc agcttggccg ggattacgtt cggcattgga
     1921 atcagaattc tccgaagcgg tagggccggg ggcgggcggc cggcgggggc gggatggagt
     1981 cgggtgaccc gttcctgccg cgggaagcca
//
LOCUS       DASUFA010000334         5131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1214321, whole genome shotgun sequence.
ACCESSION   DASUFA010000334 DASUFA010000000
VERSION     DASUFA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214321"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFX87_12315"
     CDS             <1..900
                     /locus_tag="VFX87_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995755.1"
                     /translation="GAKVRIGNREDEYAEQARAEGFEVVSIAAAAADDVVYVLLPDEV
                     IPTVFDAEIAPTLRAGSAIAFGSGYSLAFGLIHPPETIDVLLVAPRMAGTTARNRYLE
                     GHGFWACVGVEANRSGRAEQRMLGLADGMGVLRKGAVQMTAKVEATLDLFIEQTVGSM
                     LGMAIMMAFEVARDAGMPAEALVLEMYMSGEMEAVFQSFRETGFFRASEDHGPTAVFG
                     GITRSLEMDRDAIAETFRRTLEDIKSGGFAQRFQEEARNGYPMLEFARAMMHGGSPIT
                     DAEDRIRHLTSPDSSGASPAGSA"
     gene            complement(922..1551)
                     /gene="pdxH"
                     /locus_tag="VFX87_12320"
     CDS             complement(922..1551)
                     /gene="pdxH"
                     /locus_tag="VFX87_12320"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX4995756.1"
                     /translation="MSAPLPEILPADPLPLLAQWLEEARPAVKTPTAMALATVDAAGR
                     PTARMVICRGFDVSLGWFVFYSDRESTKGQALAANPRAALVFHWDVFERQIRVEGPVT
                     HAPDADSDRYWSTRPLDARVAAAASEQSRPIASRRAFLEKIEATKQTHGADVPRPKRW
                     GGYRVWAERVELWAGQPGRAHDRAVWTRALQPAGAGFTGGSWRGTRLQP"
     gene            complement(1570..2013)
                     /locus_tag="VFX87_12325"
     CDS             complement(1570..2013)
                     /locus_tag="VFX87_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995757.1"
                     /translation="MTPLIRRYIKTSFAFLLAGLLIGAYIVVGQFVLDAYPPRLLITA
                     HAHLLLVGFMLMMVMGVATWMFPRPARDDTRYRTELAEAVYWVMTISTTLRAAAEISV
                     ALAGALGLRWVIALGGLGQLAGTALFVLNMWWRVRMPPAAAPPVR"
     gene            complement(2015..2716)
                     /locus_tag="VFX87_12330"
     CDS             complement(2015..2716)
                     /locus_tag="VFX87_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4995758.1"
                     /translation="MSVKAIAALLGGNLVFAGIPARELEALARVTLEEAHRARGEIFR
                     EGDMARWFYLVRSGHVKIVRHSKTGKDVVLELLGPGEIFGGVAVIEKRPYPASAQATE
                     PTVVLKIPADPVIALAERHPAFIKEMALMIGRRLRAAHDSVASLAVDPVEARLAAILL
                     RLAKREGTRSDKGVTLPFHLTRQSLADMTGTTVETSIRILSRWLRDGLLEDDDGRLVL
                     ADWKGLSALAEGAAD"
     gene            2706..3347
                     /locus_tag="VFX87_12335"
     CDS             2706..3347
                     /locus_tag="VFX87_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995759.1"
                     /translation="MTDIGGRIARGICREIAAAAAAVPHVARRGAFPSARIRGRLRWH
                     LACKSTGQGERTMRGLVELLLLGLLVGAPDRAGATSIIDGPTVLQQSPAAIQATLGRP
                     IRTKAVPSGDVHLPGGGTSRVYVGHGTRIDIDFEKERSTTVVIAFPDSTMAPRTYEAA
                     LEAVNLPSGPAPDLIRRDRREWRNLEGYFVRVIAAYPTLDHIDAIVLSVHPLP"
     gene            3514..4026
                     /locus_tag="VFX87_12340"
     CDS             3514..4026
                     /locus_tag="VFX87_12340"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX4995760.1"
                     /translation="MMPSDIPVAQVHLDVREDIRRGQEPFARIMAAVKALADDQALVL
                     RAPFEPIPLYDVLGKRGFVHRTERRASDDWSVWFYRDPAARAEPTAAPATPAPRRAVL
                     DVRGLEPPQPMLRVLQEIDRLGPGAELEVHHDRRPMFLYPQLDERGFVHETDEPEPGL
                     VRVLIRRGAG"
     gene            4023..>5131
                     /locus_tag="VFX87_12345"
     CDS             4023..>5131
                     /locus_tag="VFX87_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995761.1"
                     /translation="MSGLSPLVPLAYLVCAAVAFLAAALGVAWLGPELAGHYYHPRLL
                     ALTHTVTLGWVTLAIMGASYQLIPIVLERPIWSERLARWQLAVLAVAVTGMVAHFYLG
                     TWPGLAAAAGLLAVGIALHLGNVGMSLRGFTRWTFTARLVVLAYGGLALTTLLGLALA
                     GNRVWPFLPGELFPTLHAHVQLALLGWVAPMILAVAARVYPMFFLAPAPRRWHSALQF
                     WGLAAGVPAVVLGLLGVPGLLVAGTLAVAAAAGAHGAWMSEMASRRRRPGLDWGLRFV
                     LTAAAFLLPAAVLGVALAANRLSGPRAAIAYAVVTLGGWVSLTIAGMMLKIVPFLVWY
                     RAYSPRAGREPVPTLAQISWPRAEGLAYGLLTGGVV"
BASE COUNT          684 a         1873 c         1819 g          755 t
ORIGIN      
        1 ggggcgaagg tgcgcatcgg caatcgcgag gacgagtacg ccgagcaggc gcgcgccgag
       61 ggcttcgagg tggtgtcgat cgccgccgcg gccgccgacg acgtggtgta cgtgctgctg
      121 cccgacgagg tcatcccgac cgtcttcgac gcggagatcg cgcccacgct gcgagcgggc
      181 agcgccatcg cgttcggctc gggctacagc ctggccttcg gcctcatcca cccgcccgag
      241 acgatcgacg tgctgctggt ggccccgcgc atggccggga ccaccgcgcg caaccgctat
      301 ctcgaaggcc acgggttctg ggcctgcgtg ggcgtggagg ccaaccgcag tggccgggcc
      361 gagcagcgga tgctcgggct cgccgacggc atgggcgtgc ttcgcaaggg cgcggtccag
      421 atgaccgcga aggtcgaggc cacgctcgac ctcttcatcg agcagacggt gggctcgatg
      481 ctgggcatgg cgatcatgat ggcgttcgag gtcgcgcgcg acgccgggat gccggccgag
      541 gcgctggtgc tcgagatgta catgtcgggc gagatggagg cggtcttcca gtccttccgc
      601 gagaccggct tcttccgcgc ctccgaggat cacggaccca ccgcggtgtt cggcggcatc
      661 acccgctcgc tggagatgga ccgcgacgcc atcgcggaga ccttccgcag gaccctcgag
      721 gacatcaaga gtgggggctt cgcgcagcgc ttccaggaag aggcccggaa cggctatccg
      781 atgctggagt tcgcgcgggc catgatgcac ggcggctcgc cgatcaccga cgcggaggac
      841 cgcatccggc acctgacctc ccccgactcg tccggcgcca gcccagccgg gtcagcatga
      901 gcttcgcccg gcgcggccgc gtcacggctg aagccgcgtc ccccgccacg acccgccggt
      961 gaagcccgcc ccggcaggct ggagcgcccg cgtccacacc gcgcgatcgt gggcgcgccc
     1021 cggctgcccc gcccacagct ccacccgctc cgcccagacc cggtagccgc cccagcgctt
     1081 gggccgtggc acgtccgcgc catgcgtctg cttcgtcgcc tcgatcttct cgaggaacgc
     1141 ccggcgcgag gcgatgggcc gactctgctc gctcgccgcc gccgcgactc gcgcgtccag
     1201 cggccgcgtg ctccagtagc ggtccgagtc cgcgtcgggc gcgtgcgtga cgggcccctc
     1261 cacgcggatc tgccgctcga atacatccca gtggaagacg agcgcggcgc gtggattggc
     1321 ggccagggcc tggcccttgg tgctctcgcg atcggagtag aagacgaacc agcccaggct
     1381 cacgtcgaag ccccggcaga tgaccatccg cgccgtcggg cggccggcgg cgtccacggt
     1441 ggccagggcc atcgcggtcg gagtcttcac cgcgggcctg gcctcctcga gccattgcgc
     1501 gagcagcggc agcggatcgg cgggcaggat ctcgggaagc ggcgcgctca tgatcgtgtc
     1561 cgcgggtcgt cagcggacgg gcggcgcggc cgcggggggc atgcgcacgc gccaccacat
     1621 gttcagcacg aacagcgcgg tccccgcgag ctggccgagc ccgcccagcg cgatgaccca
     1681 ccgcaggccg agggctcccg ccagcgccac gctgatctcg gcggccgcgc gcagcgtcgt
     1741 cgagatcgtc atgacccagt acaccgcctc ggccagctcc gtccggtacc gggtgtcgtc
     1801 gcgcgcgggc cgcgggaaca tccaggtggc gacgcccatg accatcatca gcatgaaccc
     1861 gacgaggagc aggtgggcgt gggccgtgat gagcaggcgg ggcggatacg cgtcgagcac
     1921 gaactggccg accacgatat aggccccgat caggagcccg gcgaggagga aggcgaagct
     1981 ggtcttgatg taccggcgga tcagcggggt catgctagtc ggcggcgcct tcggccagcg
     2041 cgctgagtcc cttccagtcg gcgaggacga gccggccgtc gtcgtcctcg agcaggccgt
     2101 cccggagcca gcggctcagg atccggatgg aggtctcgac cgtggtgccg gtcatgtcgg
     2161 cgaggctctg ccgggtgagg tgaaacggca gcgtgacgcc cttgtcactt cgtgtcccct
     2221 cccgctttgc gagccggagc aggatcgcgg ccaggcgcgc ctcgaccgga tccaccgcga
     2281 gcgacgcgac cgagtcgtgg gcggcgcgga gccgacggcc gatcatgagc gccatctcct
     2341 tgatgaacgc ggggtgccgc tcggcgaggg cgatcacggg atcggccgga atcttcagga
     2401 ccaccgtggg ctcggtggcc tgggcgctcg ccgggtaggg gcgcttctcg atcaccgcca
     2461 cgccgccgaa gatctcgccg ggccccagga gctccaggac cacgtccttc cccgtcttgg
     2521 agtgcctcac gatcttcacg tggcccgatc tcaccaggta gaaccagcgt gccatgtcgc
     2581 cttcccggaa gatctcgccg cgggctcgat gagcctcctc cagcgtgact cgcgccagtg
     2641 cctcgagctc gcgcgccggg atgccggcaa acaccaggtt cccacccagc aacgcggcga
     2701 tcgccttgac cgacatcggc ggcaggatag cacgaggcat ctgtcgcgag attgccgcgg
     2761 ctgcggcggc ggtgcctcac gtggccaggc gcggggcatt cccaagtgcc cggattcgcg
     2821 gccgtctgcg ctggcacctc gcgtgcaaaa gcaccggcca gggggaacga acgatgcgcg
     2881 gactcgtgga gttgttgctt ctcgggctgc tggtcggagc gccggaccgg gcgggggcca
     2941 catcgatcat cgacgggcca accgtcctcc agcaatcgcc ggccgcgatc caggccacac
     3001 tcggacgacc catccgcacg aaggcggtcc cctcggggga tgtccatctg cccgggggcg
     3061 gcacctcgcg cgtctacgtc ggccacggga cgcggatcga tatcgatttc gagaaggagc
     3121 gcagcaccac ggtcgtcatc gcctttcccg acagcacgat ggcacccagg acctatgagg
     3181 ccgcgctcga agcggtcaat ctcccgtccg gtccagcccc cgacctgatc cggcgagatc
     3241 gccgcgaatg gcgcaacctc gagggctact tcgtgcgagt gatcgccgcg tacccgacgc
     3301 tcgaccacat cgacgcgatc gtgctcagcg tccaccccct cccctagctc gtcgctccct
     3361 cgactatgac cgccgtcata ggaagtcttg ccgcccggcg gtagcgtcgg aggcagggag
     3421 aaacgacatg gccgacgagt gcagctgccg ccacgattcg ccgatggccc tcggcacggc
     3481 cggtgcgccg gtccgcgccg agcaggcgcc cgcatgatgc cgagcgacat cccggtcgcg
     3541 caggtgcacc tggacgtgcg cgaggacatc cggcgcggtc aggagccgtt cgcgcggatc
     3601 atggccgcgg tcaaggcgct cgccgacgat caggcgctgg tgctacgcgc cccgttcgag
     3661 ccgatccccc tctacgacgt gctcggcaag cgcggcttcg tccaccggac cgagcgccgc
     3721 gcctccgatg actggtcggt ctggttctac cgtgaccccg cggcgcgggc ggagccgacc
     3781 gcggctcccg ccacgcccgc gccccgccgc gcggtgctgg atgtccgggg cctcgagccg
     3841 ccgcagccga tgctgcgggt gctccaggag atcgaccggc tcggccccgg agccgagctg
     3901 gaggtgcacc acgaccgccg ccccatgttc ctctatcccc agctcgacga gcggggcttc
     3961 gtgcacgaga ccgacgagcc ggagccgggg ctcgtgcgcg tcctcatccg caggggagcg
     4021 gggtgagcgg cctctcgccg ctcgtcccgc tcgcctacct ggtctgtgcc gcggtcgcgt
     4081 tcctggcggc cgcgctgggc gtcgcatggc tcgggcccga gctggcaggc cactactacc
     4141 atccgcgcct gctcgccctc acccacaccg tcacgctcgg gtgggtcacg ctcgcgatca
     4201 tgggcgccag ctaccagctc atcccgatcg tgctcgagcg cccgatctgg agcgagcggc
     4261 tcgcccgctg gcagctcgct gtcctcgccg tcgcggtgac gggcatggtc gctcatttct
     4321 acctcggcac ctggcccggg ctggcggcgg cggcgggact gctggccgtc ggcatcgcgc
     4381 tccatctcgg caacgtcggg atgagcctgc ggggcttcac ccgctggacg ttcaccgccc
     4441 gcctggtggt cctggcatac ggcggcctcg cgctgacgac cctgctcggc ctcgcgctgg
     4501 cgggcaaccg cgtgtggccg ttcctccccg gcgagctctt cccgacgctg cacgcccacg
     4561 tgcagctggc cctcctcggc tgggtggcgc cgatgatcct cgccgtggcc gcgcgtgtgt
     4621 acccgatgtt cttcctcgcg cccgcgccgc ggcgctggca ctccgccctg cagttctggg
     4681 ggctggcggc cggtgtgccg gcggtggtgc tggggttgct tggcgtgccc ggcctgctcg
     4741 tcgccgggac cctggccgtg gcggcggcgg ccggagccca cggggcctgg atgtccgaga
     4801 tggcgagcag gcggaggcgg cccggtctcg actgggggct ccgcttcgtg ctcaccgccg
     4861 ccgcgttcct cctgcctgct gccgttctcg gcgtggcgct ggcagcgaac cggctgtcgg
     4921 ggccgcgcgc ggccatcgcc tatgcggtcg tgacgctcgg cggctgggtc tccctcacca
     4981 tcgcgggcat gatgctcaag atcgtcccgt tcctggtctg gtaccgcgcg tacagcccgc
     5041 gggcgggccg ggagccggtg cccacgctgg cgcagatctc ctggccgcgc gccgaggggc
     5101 tggcctacgg gctcctcacc ggaggcgtgg t
//
LOCUS       DASUFA010000335        10841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1214546, whole genome shotgun sequence.
ACCESSION   DASUFA010000335 DASUFA010000000
VERSION     DASUFA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214546"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFX87_12350"
     CDS             <1..561
                     /locus_tag="VFX87_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995762.1"
                     /translation="EPGRGSTFTVYLPRVAAPLATTERPAAEAPAPRPGAAPEATLTP
                     GRGETILLVEDAQRVRAVVREILEMSGYVVLEARQGAEALEISNRHAGPIHLLVTDVV
                     MPQMSGRELAQRLATLRPELKVLYMSGYTDDAIVRHGVLASGIAFLSKPFTPNALALK
                     VREVLDGVADGAAAGPSVTPGGHGRP"
     gene            592..999
                     /locus_tag="VFX87_12355"
     CDS             592..999
                     /locus_tag="VFX87_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX4995763.1"
                     /translation="MERPIRVLVAKVGLDGHDRGAKIVARCLRDAGMEVVYTGLHRTP
                     EEVVAAAVQEDVDVLGISILSGAHLTLFPRVLELLRGARAQDILLVAGGVIPDEDVPV
                     LRDMGVAEVILQDTPPDTIVARIRDLVAARPTR"
     gene            1046..2464
                     /locus_tag="VFX87_12360"
     CDS             1046..2464
                     /locus_tag="VFX87_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX4995764.1"
                     /translation="MDLTVWPPRYDPAYRPAPAAEHWLPEIECAEPAARDALIFAKLT
                     RQVRYAWERSAFYRRRWQTAGVSPETLKSLEDLARFPVVQKAELRAAQAAAPPFGDYL
                     CIEPTEVARIHGTSGTTGRPTVFGIGADDWERIGEAHARILWGAGIRPGDRILICSFF
                     SLYLGSWGALAGGERLGATVFPFGAGAQGQTLQAVQWARDLRPSAFYGTPSYALHFAE
                     TARREGIDPAGLGFRVLFFSGEPGAGIPATRALIEQTFGGICVDMGSMAEMSPWMTNG
                     ECRHRTGMHLWQDLVYTQVCDPETFHPLPYGAEGTPVYTHLERTSQPMIRLVSGDRAR
                     WLDEPCPCGRTYPRLPDGLYGRFDDMIIVRGENIYPSAIEDTLRAIDGFGGEFRVIVS
                     RREAMDELLVRAEYTAGHGARLDALRALMRERLRTRLGVHPVVDLVPEGTLPRTEFKA
                     RRVIDDRDLYRKALQTGDNRHV"
     gene            2457..4139
                     /locus_tag="VFX87_12365"
     CDS             2457..4139
                     /locus_tag="VFX87_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX4995765.1"
                     /translation="MFDPEALKRLAAEKAAWEAGELADFLARQPEARPDYRTGSGLPV
                     QRLYTPEDIADAPADNIGLPGRFPYTRGPYPTMYRGRNWTMRQIAGFGTGEDTNGRFR
                     YLIEQGQTGLSVDFDMPTLMGYDSDDPRSLGEVGREGVAIDTLDDMEALFAGIDLERT
                     SVSMTINPSAWILLAMYVALAESRGFDLNKLSGTVQADILKEYVAQKEWIFPIRPSMR
                     VMRDMIVWSARNLARYNPINISGYHISEAGATSVQELAFTMANAIAYVEEVTRAGVPV
                     DEFAPRLAFYFVAQADFFEEVAKFRAARRIYARLMKERFGATKAESMRLRFHCQTAAA
                     TLTKAQPLNNIIRTALQALSAVLGGAQSLHTNGMDEAYAIPSEEAMKIALRTQQIIAM
                     ETRVPQVVDPLGGSYYVEALTNRIEAEVLRILAKVDAMGGTIKAIEEGFFQHEIADSA
                     YDFARRKAAGEQPVIGVNTLVEPSEPPPLPIHKVDAAVEARQIGRLKETRRRRDQALV
                     DRLMDRLEREAREPATNIMPVTIELVKARASLGEIVARLRHVWGAYIERPVF"
     gene            4139..5284
                     /locus_tag="VFX87_12370"
     CDS             4139..5284
                     /locus_tag="VFX87_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX4995766.1"
                     /translation="MPPRPFRIEVPESTLADLRERLGRARWPDQAPGAEWAYGTSLAY
                     MKELTAYWKDRYDWRAHEARLNGFRQLTVPVAGIELHFIHEEGRGPNPLPLLLSHGWP
                     GSVWEFHKILPLLTDPARFGGDPADAFTVIAPSLPGYGFSFVPGQPRFGVAEIADAFA
                     ALMTDVLGYRRFAAQGGDWGGFITSRLGAAYPERLAAIHVTLLSLRRDIKPPAQPSPE
                     EKQYLDDLARWQREETGYQWIQGTRPQTLAFGLHDSPVGLAAWIVEKFRTWSDCGGDV
                     ERRFTKDELLTNVMIYWVTGSINASFWPYYARFHSPWPIADGQRIEVPTGYAAFPKEI
                     VRPPRAWAERMYNIRRWTVMPAGGHFAAMEEPDALATELRAFVREYR"
     gene            5505..6422
                     /locus_tag="VFX87_12375"
     CDS             5505..6422
                     /locus_tag="VFX87_12375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4995767.1"
                     /translation="MKLGFALPTAGDWATPENQVRVARQAEALGYHSLWVFQRLLYAL
                     EPKNEYPPVPGPVWPKPFERVFDPIVTLAYVAAATQRIRLGTSVLIMPFYQPVVLAKQ
                     LATLDVLSGGRLDVGLGLGWSQDEYEAVGVPFKHRGRRADEFLRCLDAIWTQDVTEFH
                     GEFYNVPRSRVEPKPVQKPRPPITIGGYGSTVVKRAVELGDGFNGGNVAMASVAPLVK
                     EVKDAAAALGKDPARLHIVCRGTYRVHDAPQGQDRRPLWGTLDEIREDIGRYAEAGLT
                     ELFLEGNFTPGGPSLEQALEVMARLAPGR"
     gene            6627..7439
                     /locus_tag="VFX87_12380"
     CDS             6627..7439
                     /locus_tag="VFX87_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995768.1"
                     /translation="MDDSLPSILCVDDDVDVLELLKEYFTEQGFVVLTATNGVEAFLQ
                     VKRWQPKAVILDLFMPRLGGIGALGRIKALNPDVVVILVSGMGNALELVTEAGLSVAG
                     AFTKPLDLMQLSETLARSGVVAPAALVAVATGDTGRAKRPVRARVLVVDDELEFRKVL
                     AEHLEDRGFDMMEAQDGEEALLRVPDFHPHIVLMDVMMTGMGGVEALRRIKTTAPETC
                     VIMVTAVEDIDSARGALALGASDYVTKPFSFQYLDSVLEVHMLMDHIDPRSS"
     gene            complement(7436..8833)
                     /gene="hemG"
                     /locus_tag="VFX87_12385"
     CDS             complement(7436..8833)
                     /gene="hemG"
                     /locus_tag="VFX87_12385"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX4995769.1"
                     /translation="MKLIVVGGGVSGLAAAHRAVELARERGRPLELTLLEGADRLGGT
                     IQTERRDGFLVECGPDSFLSEKPWALALCRRLGVEDRLVGTDDRFRRVFVLFRGRLHP
                     LPDGFQLLAPTRLRPFLASSLFSWPGKLRMACDLLLPRGGSPDESLGAFVRRRLGREA
                     LERVAQPLVAGIYTADPDDLSLGATMPRFLEMERRERSVILALWRAARRTPALHAGTS
                     GARWSLFVTFADGMEEMIRLLATRLPPGAVRLKERVASVARDGDGWRVSTAGGAVFTG
                     DALILAPEAHQVARLLRYVDPGLAHLLEGIPYASAATVTLAYRRADIAHPLDGFGFVV
                     PQVERRPVIAGTFSSVKYPGRAPDGHALLRVFLGGALNESALEADDTALVETARGELG
                     KVLGVTGAPLFTRVARHPHAMPQYHVGHAARAEAIERAVARHAGLRLAGGAYRGVGIA
                     DCVRSGEAAAEGVAG"
     gene            complement(8830..9750)
                     /gene="hemH"
                     /locus_tag="VFX87_12390"
     CDS             complement(8830..9750)
                     /gene="hemH"
                     /locus_tag="VFX87_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX4995770.1"
                     /translation="MIDAVLLIAVGGPERPEAVRPFLQLVAAGRRIPPERLEEVAHHY
                     DLMGGRSPLNELTRRQSEGLRAALAAAGRAVPVWVGMRNWHPFLHETLAEMKDRGCRR
                     ALGIILSSLVTEASWSRYVEDVAAAREKVGPDSPEIVYAPPWSEHPRFIEAMADRARA
                     ALAEIEPARRAAARLVFTAHSVPVSMAAGSPYAAQLETAARAIASALGRPEWSAAYQS
                     RSGSPRDPWLEPDVVDVIRALARDGARDVVVVPVGFVCDHVEVLYDLDVEARAVADSC
                     GIGFHRAGAANDHPAFIAMLADLVRRGPVP"
     gene            complement(9747..10784)
                     /gene="hemE"
                     /locus_tag="VFX87_12395"
     CDS             complement(9747..10784)
                     /gene="hemE"
                     /locus_tag="VFX87_12395"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX4995771.1"
                     /translation="MSPAVSAAFLDACLRRPTPFTPIWLMRQAGRYMPEYRAVRARLG
                     FLELCKSPEAAAEVTLQPVERLGVDAAILFADILLVLEPLGVGLEFTRGDGPRIERPV
                     RSAAAARALPAVDVEDAVGFVFETVRRVRKALAERVPLIGFAGAPFTLASYLIEGGPS
                     REFLLTKTFMRREPEAWAAMMTRLADITAEYLNGQIAAGVQAVQLFDSWVGALSPADY
                     RDFVLPHSRRVIGALRPGVPVIHFGVGTGGLLSLMKAAGGDVIGLDWRVELGPTWARL
                     GHDVAVQGNLDPAVLLTDVPAIRRAAREILDAAAGRPGHVFNLGHGIHKDTPVEHVKA
                     LVDIVHELSAR"
BASE COUNT         1591 a         3966 c         3840 g         1444 t
ORIGIN      
        1 gagccggggc gcggctccac gttcaccgtc tacctcccgc gggtggccgc cccgctcgcg
       61 acgacggagc ggcccgccgc ggaggcgccg gccccgaggc cgggtgccgc ccccgaggcc
      121 accctcaccc cggggcgcgg cgagaccatc ctgctcgtgg aggacgccca gcgcgtgcgc
      181 gcggtggtgc gggagatcct cgagatgagc ggctacgtcg tgctcgaggc gcggcagggg
      241 gccgaggccc tcgagatcag caaccggcat gcgggcccca tccacctgct agtgaccgac
      301 gtggtgatgc cgcagatgag cggccgcgag ctggcgcagc ggctggcgac cttgcgcccg
      361 gagctgaagg tgctgtatat gtccggctac acggatgacg ccatcgtcag gcacggcgtg
      421 ctggcctcgg gtatcgcgtt tctctccaag ccgttcaccc cgaacgcgct cgcgctcaag
      481 gtgcgcgagg tcctcgacgg cgtggccgac ggggcagccg ccggcccctc cgtcacgccc
      541 ggcggccacg gccgccccta gcgcctccgc accccgaccg ctcccgtcat gatcgagcgc
      601 cccatccgcg tcctggtggc caaggtcggc ctcgacgggc acgaccgcgg tgcgaagatc
      661 gtggcccgct gcctgcgcga cgcgggcatg gaggtcgtct acaccgggct gcaccgcacg
      721 cccgaggaag tggtcgcggc ggcggtgcag gaggacgtgg acgtgctcgg catcagcatc
      781 ctctccggcg cccacctcac cctcttcccg cgcgtgctcg agctgctgcg cggggcgcgc
      841 gcgcaggaca tcctgctcgt ggcgggcggg gtgatcccgg acgaggacgt gcccgtgctg
      901 cgcgacatgg gcgtggctga ggtgatcctg caggacacgc cgcccgacac gatcgtcgcc
      961 cggatccgcg acctggtagc ggcccggccg acccggtgag cccaggaccg aggcggacgc
     1021 ggccgcgctc cgccggacgc gcggcgtgga tctgacggtc tggccgccgc gctatgaccc
     1081 cgcctaccgg ccggcccccg ccgccgagca ctggctgccc gagatcgagt gcgcggaacc
     1141 cgccgcgcgc gacgcgctga tcttcgccaa gctcacgcgg caggtgcgct acgcctggga
     1201 gcggagcgcc ttctaccggc gcaggtggca gacggcgggc gtgtcgccgg agacgctcaa
     1261 gagcctcgag gacctcgcgc gcttcccggt ggtgcagaag gcggagctgc gcgccgccca
     1321 ggcggccgcg ccgcccttcg gcgactatct ctgcatcgag cccacggagg tggcgcgcat
     1381 ccacggcacg agcggcacca cgggtcggcc caccgtcttc ggcatcggcg ctgacgactg
     1441 ggagcggatc ggggaggccc acgcccgcat cctctggggc gcgggcatcc ggccgggcga
     1501 ccgcatcctg atctgctcgt tcttcagcct ctacctgggc tcgtggggcg cgctcgcggg
     1561 cggcgagcga ctcggcgcca ccgtgttccc cttcggcgcg ggcgcgcagg gccagacgct
     1621 gcaggccgtg cagtgggcgc gcgatctccg gccgtccgcg ttctacggca cgccgtccta
     1681 cgcgctgcac ttcgcggaga ccgcgcgccg cgagggcatc gacccggcag gcctcggctt
     1741 ccgcgtcctc ttcttctccg gcgagccggg cgcgggtatc ccggcgaccc gcgcgctgat
     1801 cgagcagacc ttcggcggca tctgcgtgga catgggcagc atggccgaga tgtcgccgtg
     1861 gatgaccaac ggcgagtgcc gccatcgcac cggcatgcac ctctggcagg atctggtcta
     1921 cacccaggtc tgcgatccgg agacgttcca ccccctgccc tacggcgccg agggcacgcc
     1981 cgtgtacact cacctcgagc ggacgtcgca gccgatgatc cggctggtct cgggcgatcg
     2041 cgcgcggtgg ctggatgagc cgtgcccgtg cgggcggacc tacccgcgcc tgccggatgg
     2101 gctctacggc cgcttcgacg acatgatcat cgtgcgcggc gagaacatct acccgagcgc
     2161 catcgaggat accctgcgcg ccatcgacgg cttcggcggc gagttccggg tgatcgtctc
     2221 ccggcgcgag gccatggacg agctgctggt gcgcgccgag tacaccgcgg gccacggcgc
     2281 gcgcctcgac gcgttgcgcg cgctgatgcg cgagcgcctg cggacgcgcc tcggcgtgca
     2341 cccggtggtg gatctcgtcc ccgaggggac gctcccgcgc accgagttca aggcccggcg
     2401 cgtgatcgac gatcgagatc tttatcgcaa agcactacag accggagaca accgccatgt
     2461 ttgatcccga agcgctcaag cgcctcgccg ccgagaaggc cgcgtgggag gccggggagc
     2521 tcgccgactt cctcgcccgc cagccggagg cgcgccccga ctaccggacc ggctcgggcc
     2581 tgccggtgca gcgcctctac accccggagg acatcgcgga cgcgcccgcc gacaacatcg
     2641 gcctgcccgg ccgcttcccc tacacgcgcg ggccctatcc caccatgtac cgcggccgca
     2701 actggaccat gcgccagatc gcaggcttcg gcaccgggga ggacaccaac gggcgcttcc
     2761 gctacctgat cgagcagggg cagaccgggc tcagcgtgga cttcgacatg ccgacgctga
     2821 tgggctacga ctccgacgac ccccgctcgc tcggcgaggt cgggcgcgag ggcgtggcca
     2881 tcgacaccct cgacgacatg gaagcgctct tcgcgggcat cgacctcgag cgcacctcgg
     2941 tctccatgac catcaatccg agcgcatgga tcctgctcgc catgtacgtg gcgctcgccg
     3001 agtcgcgcgg cttcgacctg aacaagctgt ccggcaccgt gcaggccgac atcctgaagg
     3061 agtacgtcgc ccagaaggag tggatcttcc cgatccgccc ctcgatgcgc gtgatgcgcg
     3121 acatgatcgt gtggagcgcg cggaacctcg cccgctacaa ccccatcaac atcagcggct
     3181 atcacatctc ggaggcgggg gcgacctcgg tgcaggagct ggccttcacg atggcgaacg
     3241 cgatcgccta cgtggaggag gtgacccgcg cgggggtgcc cgtcgacgag ttcgcgccgc
     3301 gcctggcctt ctacttcgtg gcccaggccg acttcttcga ggaggtggcc aagttccgcg
     3361 cggcccggcg catctacgcc cgcctcatga aggagcgctt cggggccacg aaggccgagt
     3421 ccatgcgcct gcgctttcac tgccagaccg cggcggccac gctcaccaag gcgcagccgc
     3481 tcaacaacat catccgcacc gcgctgcagg cgctctccgc ggtgctgggc ggggcccagt
     3541 cgctccacac caatggcatg gacgaggcct acgcgatccc ctccgaggag gcgatgaaga
     3601 tcgcgctgcg cacccagcag atcatcgcga tggagacgcg cgtgccccag gtggtggatc
     3661 cgctcggcgg gtcctactac gtggaggccc tgaccaaccg gatcgaggcc gaggtcctcc
     3721 gcatcctcgc caaggtggac gcgatgggcg gcaccatcaa ggccatcgag gagggcttct
     3781 tccagcacga gatcgccgac tccgcctacg acttcgcccg gcgcaaggcg gcgggcgagc
     3841 agcccgtgat cggcgtcaat acactggtag aaccgtcgga gccgccgccg ctgcccatcc
     3901 acaaggtgga cgccgccgtc gaggcgcgcc agatcggccg gctgaaggag acgcggcggc
     3961 ggcgcgacca ggcgctcgtg gaccgcctga tggaccggct cgagcgcgag gcgcgcgagc
     4021 ccgcgaccaa catcatgccg gtgacgatcg agctcgtgaa ggcgcgcgcc tcgctgggcg
     4081 agatcgtggc gcgcctccgt cacgtctggg gcgcctacat cgagcggccg gtgttctgat
     4141 gccgccccgg cccttccgca tcgaggtccc ggagagcacg ctggccgacc tgcgggagcg
     4201 cctcggccgc gcgcggtggc ccgaccaggc gcccggcgcc gagtgggcgt acggcacgag
     4261 cctggcgtac atgaaggagc tgaccgccta ctggaaggac cgctacgact ggcgcgcgca
     4321 cgaggcgcgg ctcaacggat tccgccagct caccgttccc gtggccggca tcgagctgca
     4381 cttcatccac gaggaaggcc gcggaccgaa cccgctcccg ctgctgctct cccacggctg
     4441 gcccggctcg gtgtgggagt tccacaagat cctgcccctg ctcaccgacc cggcgcgctt
     4501 cggcggcgat cccgcggacg cgttcacggt gatcgcgccc tcgctgcccg ggtacggctt
     4561 ctcgttcgtg cccggccagc cgcgcttcgg cgtcgcggag atcgccgacg ccttcgcggc
     4621 gctcatgacc gacgtgttgg gctaccggcg cttcgccgcg cagggcggcg actggggcgg
     4681 cttcatcacg tcgcggctcg gcgcggcata tccggagcgg ctcgccgcta tccacgtgac
     4741 cctgctgtcg ctccggcgcg acatcaagcc gccggctcag ccctcgccgg aggagaagca
     4801 gtacctcgac gatctcgccc ggtggcagcg cgaggagacc ggctaccagt ggatccaggg
     4861 cacccggccc cagaccctcg ccttcggcct ccacgactcg ccggtcgggc tcgcggcctg
     4921 gatcgtggag aagttccgga cctggagcga ctgcggcggc gacgtggagc ggcgcttcac
     4981 caaggacgag ctgctcacca acgtgatgat ctactgggtg accggcagca tcaacgcctc
     5041 cttctggccg tactacgcgc gcttccactc gccgtggccc atcgcggacg gccagcgcat
     5101 cgaggtcccg accggctacg ccgcgttccc caaggagatc gtgcggccgc cgcgcgcctg
     5161 ggccgaacgc atgtacaaca tccgccggtg gacggtgatg ccggcgggcg gccacttcgc
     5221 ggcgatggaa gagccggacg cgctcgcgac ggagctccgc gccttcgtcc gggagtaccg
     5281 ctgacgcgcc ccgggcggcg ggccgccggc ttcgtcctgg cggtcctgct gacggcgacc
     5341 ggtggacaag atccgcgaga tcgtcgcgtc ggtgaacgcg gcgcggccgg acctgatcgc
     5401 gctgctcggg gactacgtca tccacggggt ggtgggcggc cggttcgtgg acatcgagcg
     5461 aacggctcgc ggagtcgaag tagcctcgtg tatcatctcg gtccatgaag ctcggattcg
     5521 cgttgcccac cgccggggac tgggccactc ccgagaacca ggtccgcgtc gcgcgccagg
     5581 cggaagcgct cggctaccac tcgctctggg tcttccagcg cctgctctac gcgctcgagc
     5641 ccaagaacga gtacccgccg gttccgggcc cggtgtggcc caagcccttc gagcgcgtct
     5701 tcgatccgat tgtcacgctc gcctacgtgg ccgcggccac ccagcgcatc cgcctcggca
     5761 cgagcgtgct gatcatgccg ttctaccagc cggtcgtcct cgccaagcag ctcgccacgc
     5821 tcgacgtgct ctcgggcggc cgcctcgacg tggggctcgg cctcggctgg tcgcaggacg
     5881 agtacgaggc ggtcggcgtc cccttcaagc accggggtcg gcgcgccgac gagttcctgc
     5941 gctgcctcga cgcgatctgg acccaggacg tcaccgagtt ccacggcgag ttctacaacg
     6001 tgccgcgctc ccgcgtggag ccgaagccgg tccagaagcc gcggccgccg atcaccatcg
     6061 gcgggtacgg ctcgacggtg gtgaagcggg cggtggagct gggcgacggc ttcaacggcg
     6121 gaaacgtggc gatggcgagc gtggcaccgc tggtgaagga agtgaaggac gcagcggccg
     6181 cgctcggcaa ggacccggcc aggctccaca tcgtctgccg cgggacctac cgcgtccacg
     6241 acgccccgca gggccaggac cgccgccccc tctggggcac gctcgacgag atccgcgagg
     6301 acatcggccg ctacgcggag gccggcctca ccgagctgtt cctcgagggc aacttcacgc
     6361 cgggcggtcc ctcgctcgag caggcgctcg aggtgatggc gcgcctggct cccggccgct
     6421 agcgcctcgc ggcatcccgg agaacgcgcg ggcgggcggg gtgtccggcc ggggtggcta
     6481 gccctggtga ccgcgagtgt caggccccgt gccaggatat gtgaaccacg ttgcagtccc
     6541 cgcagcagcc cggaggtaaa gcgccgttat ttcgttgggt tgtcccgggt actcgcgcgg
     6601 cacttttctt gcttgaatag cgtgacatgg acgacagtct cccgagcatc ctgtgcgtcg
     6661 atgacgacgt agacgtcctc gagctgctca aggagtattt caccgagcag gggttcgtcg
     6721 ttctgaccgc cacgaacggg gtcgaagcct tcctccaggt caagcggtgg cagcccaaag
     6781 ccgtcatcct cgacctcttc atgccgcgcc tgggcggcat cggcgcgctg ggccggatca
     6841 aggcgctcaa tcccgacgtg gtcgtgatcc tggtcagcgg catgggcaat gccctcgagc
     6901 tggtcaccga ggccggcctg agcgtggccg gcgccttcac caagccgctc gacctgatgc
     6961 agctctccga gacgctggcc cggtcgggtg tggtggcccc ggcggccctg gtggccgtgg
     7021 cgacgggcga taccgggcgg gccaagcggc cggtgcgcgc gcgcgtgctc gtggtggacg
     7081 acgagctcga gttccgcaag gtgctggccg agcacctcga agaccgcggc ttcgatatga
     7141 tggaggcgca ggacggcgag gaggctctcc tccgggtgcc ggacttccac ccgcacatcg
     7201 tgctgatgga cgtgatgatg acgggcatgg gcggcgtcga ggcgctgcgc cgcatcaaga
     7261 ccaccgcgcc cgagacctgc gtgatcatgg tcaccgcggt ggaagacatc gactccgccc
     7321 gcggggcgct ggcgctcggc gcctccgact acgtgaccaa gccgttcagc ttccagtatc
     7381 tcgactcggt gctcgaggtc cacatgctga tggaccacat cgacccgagg tcgagctagc
     7441 ccgccactcc ctccgccgcg gcctcgcccg atcgcacgca gtccgcgatc ccgacacccc
     7501 ggtaggctcc gcccgccaga cgcaggcccg cgtgccgcgc caccgcgcgc tcgatggcct
     7561 ccgcgcgggc cgcgtgaccc acgtgatact gcggcatcgc gtgcgggtgg cgggcgaccc
     7621 gggtgaacag cggcgcgccc gtcacgccca ggaccttgcc gagctcgccg cgcgcggtct
     7681 ccaccagtgc cgtgtcgtcg gcctcgagcg ccgattcgtt cagcgccccg cccaggaaca
     7741 cccgaaggag cgcgtgccca tcgggggcgc gccccggata cttcacgctc gagaaggtac
     7801 ccgcgatgac gggccgccgc tccacctgcg gcaccacgaa gccgaagccg tcgaggggat
     7861 gggcgatgtc ggcgcgccgg taggcgagcg tcacggtggc ggcggacgcg tagggaatcc
     7921 cctccagcag atgcgcgagc ccgggatcca cgtagcgcag gagccgcgcc acctggtgcg
     7981 cctccggggc caggatcagc gcgtcgccgg tgaagaccgc gccgcccgcg gtggacacgc
     8041 gccagccgtc accgtcccgc gcgaccgagg ccacccgctc cttgagacgc accgctccgg
     8101 gcggcagccg ggtggcgagc aggcggatca tctcctccat cccgtcggcg aacgtcacga
     8161 agagcgacca gcgggcgccg ctcgtccccg cgtgcagggc cggggtgcgg cgggcggccc
     8221 gccagagggc caggatcacg ctgcgctcgc ggcgctccat ctcgaggaag cgcggcatgg
     8281 tggcgccgag cgagagatcg tcgggatcgg cggtgtagat cccggcgacc agcggctggg
     8341 cgacgcgctc gagcgcctcg cggccgagcc gacggcggac gaaggcgccg agactctcgt
     8401 cgggagaccc gccgcggggc agcagcaggt cgcaggccat ccgcagcttg ccgggccagg
     8461 agaagagcga cgaggccagg aacggtcgca atcgggtggg cgccaggagc tggaagccgt
     8521 ccggtagcgg atgaagccgg ccgcggaaca gcacgaagac gcgccggaag cggtcgtcgg
     8581 tcccgaccag ccgatcctcc accccgagcc gccggcagag cgcgagggcc cacggcttct
     8641 cggagaggaa cgaatcgggc ccgcactcga cgaggaagcc gtcgcgacgc tcggtctgga
     8701 tggtgccgcc caggcgatcc gctccttcga gcagggtcag ctcgagcggc cggccgcgct
     8761 cgcgcgccag ctccaccgcc cggtgggcgg ccgcgagccc ggagaccccg ccgccgacga
     8821 cgatcagctt cacggcaccg gcccccgccg gaccaggtcg gcgagcatcg caatgaacgc
     8881 gggatgatcg ttggcggcgc cggcccgatg gaagccgatc ccgcaagagt cggccaccgc
     8941 cctggcctcc acgtccagat cgtagagcac ctcgacgtgg tcgcagacaa agccgaccgg
     9001 gacgaccacc acgtcgcgcg cgccgtcccg cgccagcgcg cggatgacgt cgaccacgtc
     9061 gggctcgagc catggatcgc gcggggagcc gctgcggctc tggtaggccg ccgaccactc
     9121 gggccggccg agcgcggaag cgatggcgcg tgccgccgtc tcgagctgcg cggcgtaggg
     9181 cgagcccgcg gccatggaca ccgggacgct gtgggcggtg aacacgagcc gcgcggccgc
     9241 ccggcgcgcg ggctcgattt ccgcgagcgc ggcccgcgcg cggtccgcca tcgcctcgat
     9301 gaatcgcggg tgctcactcc agggcggggc gtagacgatc tcgggtgagt ccgggccgac
     9361 cttctcgcgc gcggccgcga cgtcctccac gtagcgcgac cacgaagcct cggtcacgag
     9421 cgacgacagg atgatgccga gcgcccggcg gcagccccgg tccttcatct cggccagggt
     9481 ctcgtgcagg aacgggtgcc agttccgcat gccgacccag accggcaccg cgcgcccggc
     9541 cgccgcgagc gcggcgcgca ggccctccga ttggcggcgg gtcaattcat tgagcggcga
     9601 gcgcccgccc atcaggtcgt aatgatgcgc gacctcctcg agacgctcgg gcgggatgcg
     9661 gcggcccgcg gcgacgagct gcagaaacgg gcgcacggcc tccgggcgct ccggtccccc
     9721 gacggcgatg aggaggacgg cgtcgatcat cgcgcggaca gctcgtggac gatgtccacg
     9781 agcgccttca cgtgctcgac cggcgtgtcc ttgtggatgc cgtgccccag gttgaacacg
     9841 tgccccggcc gcccggccgc cgcgtcgagg atctcgcggg cggcgcggcg gatcgcgggc
     9901 acgtcggtca gcagcaccgc cgggtcgagg ttgccctgca ccgccacgtc gtggccgagc
     9961 cgggcccacg tgggcccgag ctccacgcgc cagtcgagcc cgatcacgtc cccgcccgcc
    10021 gccttcatca gcgacagcag gccgccggtg cccacgccaa aatggatgac cggcacgccc
    10081 ggccgcagcg caccgatcac gcgccgcgag tgcgggagca cgaagtcgcg gtagtcggca
    10141 ggcgagagcg cgcccaccca cgagtcgaag agctgcaccg cctggacgcc ggctgcgatc
    10201 tgcccattga ggtactcggc ggtgatatcc gccaaccgcg tcatcatcgc ggcccacgcc
    10261 tcgggctccc gccgcatgaa cgtcttggtg agaaggaatt cgcgcgaggg cccgccctcg
    10321 atcaggtacg aggcgagcgt gaacggcgct cccgcgaagc cgatgagggg cacgcgctcg
    10381 gcgagcgcct tgcgcacccg ccgcaccgtc tcgaacacga agccgaccgc gtcctccacg
    10441 tccaccgcgg ggagggcccg cgcggccgcc gcgctgcgca ccgggcgctc gatgcgcggg
    10501 ccgtcgccgc gggtgaactc gagcccgacc ccgagcggct cgagcacgag caggatgtcc
    10561 gcgaagagga tcgccgcgtc cacgccgagg cgctccaccg gctggagcgt gacctccgcg
    10621 gccgcctccg ggctcttgca cagctcgaga aagccgagcc gcgcgcgcac cgcgcggtac
    10681 tccggcatgt agcggccggc ctgccgcatg agccagatcg gcgtgaacgg cgtgggccga
    10741 cggagacacg catcgaggaa ggcggcggaa acggccggcg acatgctgcc ccgaagccta
    10801 caggcggggt ctggcgcagt caaggaagcg gggctatcat c
//
LOCUS       DASUFA010000336         4931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1329895, whole genome shotgun sequence.
ACCESSION   DASUFA010000336 DASUFA010000000
VERSION     DASUFA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329895"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="VFX87_12400"
     CDS             complement(<1..1722)
                     /locus_tag="VFX87_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4995772.1"
                     /translation="MEVSGAAAVTVGNLCRSYRRGRIRAVSDVSFEVPRGEVFGLIGP
                     DGAGKTSVLQVLAGVLRADSGEVRVAGVDVIRDPEAVKEAIGYMPQGLGLNLYDSLTV
                     QENIEFFRDLRRVPRAQYGDNRERLLRMTRLERFLDRPAGQLSGGMRQKLALICTLIH
                     LPDVLLLDEPTTGVDPISRRDFWSIIHTLVASRGVTVLMTTAYMDEAERCHRVSLMHD
                     GAVIAAGRPDELTATLAGRVWSVRSPHPDDVVAALMKGEGAQSLAIAGGEVRVLARGP
                     GRDPGGELGAAGLTGFTVGAVRPSFEDVFVEYLTRELHAPPAAAPSLLAAPRVTFVGS
                     AVIADRLTCRFGRFTAVDEVSLTLDEGEILGLLGPNGAGKTTLIRMLCGLQAPAGGAA
                     TVAGFDVRRARTVLRGHIGYMSQRFSLYPDQSVLENLRLSAGLYGIPRAERAGRIGAL
                     LSSLGLESLADRQPRALPLGVRQRLALASAMLHEPRVLFLDEPTAGVDPLARRQFWDL
                     VHLLAHQHGVAVLVSTHYMDEATHCDRLGFMHEGRLVGLGRPSELTERARAEGGPMLA
                     VDAAEFAR"
     gene            complement(1713..2882)
                     /locus_tag="VFX87_12405"
     CDS             complement(1713..2882)
                     /locus_tag="VFX87_12405"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4995773.1"
                     /translation="MTPGGQESAPPGPAPARPLAGSLRGRRRWLAGGAIALVLALVAA
                     FAGPRILSWLRPAAPTGLLSASGRIEGRITTLTPKTGAWVVALQVDEGQAVRQGQLLA
                     TLDDLAQRERVRAAEEQVNALARKLVAANSQLGMIERQTTLQTEQAAAALRETEARLR
                     RARAGLRQAERDAQRVAVLVAKELIAPQEGEHARLQVEVEENGVKEAEEALVSSEKQL
                     ALARVGLQQIEIVRAERDALARQRAQAEAQLAEQRSHVADFAVRSPIDGRVLTRTVER
                     GERVEAGTPLFTLIDLDRLYVKIYVPEPSIGKVALGQEARVSVDAYPGRAFPARVSRV
                     SQEAEFTPKNVETKEERVKLVFAVEVALGENPGGVLKPGMPADAVIRWQPDAPWR"
     gene            3146..4504
                     /locus_tag="VFX87_12410"
     CDS             3146..4504
                     /locus_tag="VFX87_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX4995774.1"
                     /translation="MPPPTALAPGAWHYFALFTMVIAALITEPLPGPVVGILGITAAT
                     ALGLVAPAPDDAIRWALSGFSHSTVWLIFVAFMFGLGYEKTGLGRRIALGLVRGLGRR
                     TLGLGYAVALADLALAPFTPSNTARSGGTIYPIIKCIPPLYDSSPGRTAGRIGAYLMW
                     TAFAVTGITSSMFLTANAANLFAVSLMRDRAHISVGWTDWAIGFLPVGLVLFLSLPLV
                     VYLVYPPGVKVSREVPAWAAQELRRMGPVSRREATMAGIAVLALGLWIFAGRWLNPTT
                     AALIAFCLMILTGVLSWRDVLGYTDAWRVLVWFATLVTLADGLHRVGFLTWFAGRMAA
                     DLSSVPPTTRLVLFVAVFFVAHYMFASLTAHATALLPVFLAAGAAMPDLPARPLALLL
                     SYAIGLMGVLTPYATGPAPIYFASGYLSRPAFWALGGIFGAFFLLTLLALGVPWLLSG
                     IP"
     gene            complement(4396..>4931)
                     /locus_tag="VFX87_12415"
     CDS             complement(4396..>4931)
                     /locus_tag="VFX87_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995775.1"
                     /translation="PGIGPRRAAGLRAAIAAMLGRTRLRPRQPAEEPPAAMLLDVDRR
                     YRDAAAAGRLPRIAPRRFNPEGRAWLPVLHTERDRWQFTALFSNTARAHDLGKTADWV
                     VVYFHTDTQSEGQRPIVTEPSGRLAGRRVIRGRESECLAAYGMPESSQGTPSASNVSR
                     KKAPKIPPRAQKAGRLR"
BASE COUNT          623 a         1871 c         1705 g          732 t
ORIGIN      
        1 ccgggcgaac tccgccgcgt ccaccgcgag catcgggccg ccctcggccc gggcccgctc
       61 ggtcagctcg gacgggcggc cgagaccgac cagacgcccc tcgtgcatga agcccagacg
      121 atcgcagtgc gtggcctcgt ccatgtagtg ggtggagacc agcaccgcga ccccgtgctg
      181 gtgggccagc aggtgcacga gatcccagaa ctggcggcgg gcgagcgggt ccactcccgc
      241 ggtcggctcg tcgaggaaca gcaccctcgg ctcgtgcagc atcgcgctgg cgagggccag
      301 ccgctggcgc accccgagcg gcagcgcccg gggctggcgg tcggcgaggc tctcgagccc
      361 caggctcgac agcagcgccc cgatgcggcc cgcccgctcg gcccgcggga tcccgtagag
      421 accggcggac aggcggaggt tctccaggac gctctggtcc gggtagagcg agaagcgctg
      481 ggacatgtag ccgatgtggc cccgcagcac ggtccgggct cgccgcacgt cgaacccggc
      541 gacggtggcc gcgccgcccg ccggcgcctg gagcccgcag agcatccgga tcagggtcgt
      601 cttgccggcg ccgttgggcc cgaggagacc caggatctcg ccctcgtcga gggtcaggct
      661 cacctcgtcg accgcggtga agcgcccgaa ccgacacgtg aggcgatccg cgatcaccgc
      721 gctcccgacg aacgtgacgc gaggcgcggc gagcaggctg ggcgccgcgg cgggcggcgc
      781 gtggagctcc cgggtaagat actccacgaa cacatcctcg aagctcggcc ggacggctcc
      841 caccgtgaat ccggtgagac cggcggcgcc gagctcgccg cccggatcgc gtccggggcc
      901 gcgtgcgagc acgcgcacct cgccccccgc gatcgccagg gactgggcgc cctcgccctt
      961 catcaacgcg gcgacgacgt cgtcgggatg ggggctccgg acgctccaga cgcgacccgc
     1021 gagcgtggcc gtcagctcgt ccgggcgccc ggccgcgatc accgcgccgt cgtgcatgag
     1081 gctcacccgg tgacagcgct cggcctcgtc catgtaggcg gtggtcatca gcacggtcac
     1141 gccccggctc gccaccagcg tgtggatgat cgaccagaag tcgcgccggg agatgggatc
     1201 cacgccggtg gtgggctcgt ccagcaggag cacgtccggc aggtggatca gcgtgcagat
     1261 gagggccagc ttctggcgca tcccccccga gagctggccg gccgggcgat cgaggaatcg
     1321 ctccagccgg gtcatccgca gcagtcgctc gcggttgtcg ccgtactgcg cccgcgggac
     1381 ccggcgcaga tcgcggaaga actcgatgtt ctcctgcacg gtgagcgagt cgtagaggtt
     1441 gagtccgagg ccctggggca tgtacccgat ggcctccttc accgcctccg gatcccggat
     1501 cacgtccacg ccggccacgc ggacctcgcc ggaatccgcc cggagcaccc cggccagcac
     1561 ctgcagcacg ctggtcttgc cggcaccgtc gggcccgatc aggccgaaga cctcgccgcg
     1621 cggcacctcg aaggagacat cggacaccgc ccggatgcga ccccgccggt agctccggca
     1681 caggttcccg accgtgacgg cggccgcgcc gctcacctcc acggcgcgtc gggctgccag
     1741 cggatcaccg cgtcggcggg catcccgggc ttgagcacgc cgccgggatt ctcgccgagc
     1801 gcgacctcga ccgcgaacac cagcttgacg cgctcctcct tcgtctcgac gttcttcggc
     1861 gtgaactccg cctcctgcga cacgcggctc acccgggccg ggaacgcgcg gcccggatag
     1921 gcgtccacag acacgcgcgc ctcctgcccc agggccacct tgccgatgct gggctccggc
     1981 acgtagatct tcacgtagag gcggtcgaga tcgatgaggg tgaagagcgg cgtgccggct
     2041 tccacgcgct ctccgcgctc gacggtgcgg gtgagcaccc ggccgtcgat cgggctccgc
     2101 accgcgaagt ccgcgacgtg gctccgctgc tcggcgagct gggcctccgc ctgcgcgcgc
     2161 tgccgcgcca gcgcgtcgcg ctccgcgcga acgatctcga tctgctggag gcccacgcgc
     2221 gcgagggcga gctgcttctc gctgctcacg agcgcctcct ccgcctcctt gaccccgttc
     2281 tcctcgacct cgacttggag ccgggcgtgc tcgccctcct gcggggcgat cagctccttg
     2341 gcgacgagca cggccacgcg ctgcgcgtcg cgctcggcct gccggaggcc ggcgcgcgcg
     2401 cgccgcagcc gggcctcggt ctcgcgcaga gccgccgcgg cctgctcggt ctggagtgtc
     2461 gtctgccgct cgatcatccc gagctggctg ttggccgcaa ccagcttccg ggccagcgcg
     2521 ttgacctgct cttccgccgc ccgcacccgc tcgcgctggg cgagatcgtc cagtgtcgcg
     2581 aggagctgcc cctggcgcac ggcctggcct tcgtccacct gcagggcgac cacccacgcc
     2641 cccgtcttcg gcgtgagcgt ggtgatgcgc ccttcgatcc ggccactcgc cgacagcagt
     2701 ccggtcggcg ccgcgggacg gagccatgac aggatgcgag gcccggcaaa cgccgcgacc
     2761 agcgcgagca ccagcgcgat ggccccgccc gccagccagc ggcgccgccc gcgcagggag
     2821 cccgccagcg ggcgcgccgg ggccggcccc ggcggcgcgc tctcttgccc tccgggagtc
     2881 atgcatacgg cccgtcgcaa gcgatatgcc accggcaacc cactgataac gcaacatttt
     2941 gtcgagaatc gggctctgga gctggggctc tcgggcccca gtccgcagcc cctggggcac
     3001 ggaggcccca gcgccgccac acccaccagc ctcttgaaga cgggagagtg gtcggcggag
     3061 aatcgggcgc catgccgaca ggggcctagc gattcgcgtc gttcgggcgc tcacccccgt
     3121 tgctctcgga gccgcgctcc tgctcatgcc ccctccgacg gccctcgctc ccggggcgtg
     3181 gcactacttc gcgctgttca cgatggtcat cgccgcgctg atcaccgagc cattgcccgg
     3241 cccggtcgtg gggattctcg ggatcacggc ggccaccgcc ctgggcctcg tggcgccggc
     3301 gccggacgat gccattcgat gggcgctgtc cggcttctcg cacagcacgg tctggctcat
     3361 cttcgtcgcc ttcatgttcg ggctgggcta cgagaagacc ggcctgggcc ggaggatcgc
     3421 gctggggctg gtccgcgggc tcggacgccg gaccctcggc ctgggctatg ccgtcgcgct
     3481 cgccgacctc gcgctggccc ccttcacacc gtccaatacc gcccggagcg gagggaccat
     3541 ctatccgatc atcaagtgca tcccgccgct ctacgattcc tcgccggggc ggaccgcggg
     3601 acgcatcggc gcctatctga tgtggacggc cttcgcggtg accggcatca cgagctccat
     3661 gttcctcacc gcgaatgccg cgaatctctt cgcggtgtcg ctgatgcggg acagggcgca
     3721 catcagcgtc gggtggaccg actgggccat cgggttcctg cccgtcggcc tcgtgctctt
     3781 cctctccctg ccgctggtcg tctacctcgt ctatccgccc ggcgtgaagg tcagccgcga
     3841 ggttccggcc tgggcggccc aggagctgcg gcggatgggc ccggtgagcc gccgggaggc
     3901 gacgatggcg ggcatcgcgg tcctcgcgct cgggctctgg atcttcgccg gccggtggct
     3961 caatccgacc accgcggcgc tcatcgcctt ctgcctgatg atcctcaccg gcgtgctgtc
     4021 gtggcgcgac gtcctcggct ataccgacgc ctggcgggtg ctcgtgtggt tcgccacgct
     4081 cgtgaccctc gccgacgggc tccatcgggt gggattcctg acctggttcg ccggccgcat
     4141 ggccgccgat ctctcatcgg tgccgcccac caccaggctg gtgctgttcg tcgcggtgtt
     4201 cttcgtcgcc cactacatgt tcgcgagctt gaccgcccac gcgaccgcgc tgctgccggt
     4261 cttcctggcc gcgggcgcgg ccatgcccga cctgccggcc cggcccctcg ccctgctgct
     4321 ctcctatgcc atcggcctca tgggggtgct gacgccctat gcgaccggac cggcgccgat
     4381 ctacttcgcg agcggctatc tcagccggcc ggccttctgg gccctcggcg ggatcttcgg
     4441 cgccttcttc ctgctgacgt tgctcgcgct cggggtgccc tggctgctct ccggcatccc
     4501 gtaggcggcc agacactcgc tctcgcgacc gcggatcacc cgccgtccgg ccagccgacc
     4561 cgacggttcc gtcacgatcg ggcgctgtcc ctccgattgg gtgtcggtgt ggaagtagac
     4621 gacgacccag tccgccgtct ttcccaggtc gtgcgcccgg gcggtattgg agaacagcgc
     4681 cgtgaactgc cagcgatcgc gctcggtgtg gaggaccggc agccaggctc gaccctccgg
     4741 gttgaagcgg cgcggcgcga tccggggcag gcggccggcc gcggccgcgt cgcgataccg
     4801 ccggtccacg tccaggagca tcgccgccgg cggctcctcg gccggctggc gcggtcgaag
     4861 gcgggtgcgc cccagcatgg cggcgatcgc ggcacgaagc cccgcggcgc ggcgcgggcc
     4921 gatcccgggt a
//
LOCUS       DASUFA010000337         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1215112, whole genome shotgun sequence.
ACCESSION   DASUFA010000337 DASUFA010000000
VERSION     DASUFA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215112"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..878)
                     /gene="cdaA"
                     /locus_tag="VFX87_12420"
     CDS             complement(54..878)
                     /gene="cdaA"
                     /locus_tag="VFX87_12420"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX4995776.1"
                     /translation="MWPALQTFRLRDAIDIVVVAIVLYRVFVMFKETRAVQMLLGLAG
                     LMVASFVARQFDLFSTSWLLDNFWSFWVLALIVLFQPELRRALTRLGESRLLQGMALG
                     AREERGHLIDEVVKAADALASKRIGALIVLERSTGLRNYAELGVPLDAMVSADLLVSL
                     FLPYSPLHDGAAFIRGDRVAAAGCFLPLSRNTQLGRNMGTRHRAGLGLAEETDAVVLI
                     VSEEAGRISLAVDGQMESPLDLDALRRRLAELFSLEEAPLPRRTAWWEPAREWLRK"
     gene            complement(885..1736)
                     /gene="folP"
                     /locus_tag="VFX87_12425"
     CDS             complement(885..1736)
                     /gene="folP"
                     /locus_tag="VFX87_12425"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX4995777.1"
                     /translation="MNRAIVAGVSVGDGLDVAVVGALNVSPESFYSGSVVVDADRLLQ
                     AGEAMARAGAAWLDVGGMSTAPYLEARIPEALEADRLHWAVGLLTTKLDLPVSADVSR
                     VGPARAALEAGARMINDVTGLADDPALARLIAAAGAALVLMAAPTTVSSADEPVVTVR
                     TALERGLALARAAGIPDERIVVDPGIGFFRGAGVIWPEWDCRVLSGLPALRGLGRPLY
                     VGVSRKSFIGAVAGIADPAERLPGSLAATAAAVLGGAHVIRAHDVAETVQAVRVAQAI
                     RRAGERG"
     gene            complement(1918..>3190)
                     /gene="ftsH"
                     /locus_tag="VFX87_12430"
     CDS             complement(1918..>3190)
                     /gene="ftsH"
                     /locus_tag="VFX87_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX4995778.1"
                     /translation="GGKIPKGVLLVGPPGTGKTLLAKAIAGEANVPFFSISGSDFVEM
                     FVGVGASRVRDLFEQGKKHAPCIIFMDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEM
                     DGFETNEGVILIAATNRPDVLDPALLRPGRFDRQVVVARPDVKGREEILKVHARRIPL
                     APKVDLKVLARGTPGFSGADLANLVNEAALLAARQDKKVVEMIDFENAKDKVLMGVER
                     RSMIISETEKRTIAYHEAGHALVADLLPGADPLHKVTIIPRGRALGLTQQLPADDKYN
                     YSRDYLVNRITILLGGRAAEEIVFQQQTTGAGDDLEKATDMARKMVCEWGMSDRMGPL
                     TFGKGEEHIFLGREVARPKDFSEETAVLIDSEIKRIVVECATRAKHMIETNLEKLHAL
                     ARALLERESLDGEEIARILRVPPLHEAPSPA"
BASE COUNT          484 a         1147 c         1120 g          439 t
ORIGIN      
        1 catggagacg cccagcgcga gcagcttgag ctcccagtgc cgcgtgagca cgctcatttg
       61 cggagccact cgcgggccgg ctcccaccac gcggtgcggc ggggcagcgg cgcctcttcc
      121 agagagaaga gttcagccaa gcggcggcgg agcgcgtcga ggtcgagcgg cgactccatc
      181 tggccgtcca ccgcgagcga gatgcgcccg gcctcctcgg acacgatcag caccaccgcg
      241 tccgtctcct cggccaggcc caggccggcc cggtgccgcg tgcccatgtt ccggccgagc
      301 tgggtattgc gcgacagcgg caggaagcac ccggccgcgg ccacccggtc gcctcggatg
      361 aaggcggcgc cgtcgtgcag cggcgagtac ggcaggaaca ggctcacgag gaggtcggcc
      421 gagaccatcg cgtcgagcgg caccccgagc tccgcgtagt tgcgcagccc ggtgctgcgc
      481 tcgaggacga tgagggcgcc aatgcgcttg ctcgccagcg cgtcggccgc cttgaccacc
      541 tcgtcgatga ggtggccgcg ctcctcgcgc gccccgaggg ccatgccctg gagaagacgg
      601 ctctcgccga gccgggtgag ggcccggcgc agctcgggct gaaagagcac gatcagagcg
      661 agcacccaga aagaccagaa gttgtcgagc agccagctcg tgctgaagag atcgaactgg
      721 cgcgccacga acgacgcgac catgaggccg gccaggccga gcagcatctg gaccgcgcgc
      781 gtctccttga acatcacgaa gacgcggtag agcacgatgg ccaccaccac gatgtcgatc
      841 gcgtcgcgaa gccggaaggt ctggagcgcg ggccacatgg cggcctaccc gcgctcgccg
      901 gcccggcgga tggcttgggc cacgcgcacg gcctggaccg tctcggccac gtcgtgtgcg
      961 cggatgacgt gggcgccccc cagcaccgcc gccgcggtgg ccgccagcga ccccggcagc
     1021 cgctccgcgg gatccgcgat cccggccacg gccccgatga acgacttgcg ggagacgccg
     1081 acgtagagcg gtcggccgag cccgcgcagc gccggcagac cggacagcac gcggcaatcc
     1141 cactcgggcc agatcacgcc ggcgccgcgg aagaagccga tgcccggatc caccacgatg
     1201 cgctcgtcgg ggatcccggc ggcgcgcgcg agggccaggc cacgctcgag cgccgtgcgc
     1261 acggtgacca cgggctcgtc ggcggacgaa accgtggtag gcgcggccat gagcacgagg
     1321 gcggcccccg cggcggcgat caatcgcgcg agggccggat cgtccgccag gccggtcacg
     1381 tcgttgatca tgcgggcccc ggcctcgagc gcggcccggg cgggccccac acggctcacg
     1441 tcggctgaca cggggaggtc gagcttggtg gtgaggagcc ccaccgccca gtgcagtcgg
     1501 tcggcctcca gcgcctccgg gatccgggcc tccaggtagg gcgcggtgga catgcccccc
     1561 acgtccagcc aggccgcccc cgcccgggcc atggcctccc ccgcctggag cagccggtca
     1621 gcatccacta ccacggagcc cgagtagaag gactccgggc tcacgttgag cgcccccacg
     1681 acggccacgt ccaggccatc gccaaccgac actcctgcca cgatcgcgcg gttcataggg
     1741 ggattgctcc acgactaact tatcaccgca ccgggcgcct gcgggagccg gaatttgcgg
     1801 aaaaaggcca agaacgagcg tccgcggcct gggggagtgc gggggccatt tctgggcccc
     1861 cgcattggaa cgaggggcgg gttcgtgtgt cagcccaccc cggactgcat caggccacta
     1921 ggccgggctc ggcgcctcgt gcagcggcgg cacgcgcagg atgcgcgcga tctcttcgcc
     1981 gtcgagggac tcgcgctcga ggagcgcgcg cgcgagggcg tgcagcttct cgaggttcgt
     2041 ctcgatcatg tgcttggcgc gggtggcgca ctccaccacg atccgcttga tctcggagtc
     2101 gatgaggacc gcggtctcct cgctgaagtc cttcgggcgg gccacctcgc ggccgaggaa
     2161 gatgtgctcc tcgcccttgc cgaaggtgag cgggcccatc cggtccgaca tgccccactc
     2221 gcagaccatc ttgcgggcca tgtcggtcgc cttctcgaga tcgtcccccg cgccggtggt
     2281 ctgctgctgg aacacgatct cctcggcggc gcggccgccc agcaggatcg tgatgcggtt
     2341 caccaggtag tcgcgcgagt aattgtactt gtcgtcggcg ggcagctgct gggtgaggcc
     2401 cagcgcgcga ccgcgcggga tgatcgtcac cttgtgcagc ggatcggcgc ccggcagcag
     2461 atctgccacg agcgcgtgcc cggcctcgtg gtacgcgatg gtcctcttct cggtctccga
     2521 gatgatcatc gagcgccgct ccacgcccat caggaccttg tccttggcgt tctcgaagtc
     2581 gatcatctcg acgactttct tgtcctggcg cgcggccagc agggccgcct cgttcaccag
     2641 gttggccagg tcggcgccgg agaagccggg cgtgccgcgg gcgagcacct tgaggtccac
     2701 cttgggcgcg agcgggatcc gccgagcgtg caccttgagg atctcctcgc ggcccttgac
     2761 gtcggggcgc gccaccacca cctggcggtc gaagcggccg ggcctcagca gcgccgggtc
     2821 cagcacgtcg ggccggttgg tggccgcgat gaggatgacg ccctcgttgg tctcgaagcc
     2881 gtccatctcg accaggagct ggttcagggt ctgctcgcgc tcgtcgtgcc cgcccccgag
     2941 gcccgcgccc cggtgccggc cgaccgcgtc gatctcgtcc atgaagatga tacagggcgc
     3001 gtgcttcttg ccctgctcga agagatcgcg cacgcgcgag gcacccaccc ccacgaacat
     3061 ctcgacgaag tccgagccgg agatcgagaa gaacggcacg ttggcctcgc ccgcgatggc
     3121 cttggccagg agcgtcttgc cggtcccggg agggcccacc aggagcaccc ccttggggat
     3181 cttgcccccg
//
LOCUS       DASUFA010000338         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_645963, whole genome shotgun sequence.
ACCESSION   DASUFA010000338 DASUFA010000000
VERSION     DASUFA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645963"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFX87_12435"
     CDS             <1..415
                     /locus_tag="VFX87_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4995779.1"
                     /translation="ALSTQDPEKTAKFYVDVFGMKQIGRVDHPAVSGFFLTDGDLNLA
                     VLKFKTDAVAGVERGRDFSGIHHIGFQVDSLEEIALRLAAAGAARRDDVNDALGVGHQ
                     EKRYVNVEVKYSGPDGVMVDVSETGWVGASGFRPQ"
     gene            427..1230
                     /locus_tag="VFX87_12440"
     CDS             427..1230
                     /locus_tag="VFX87_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995780.1"
                     /translation="MPKRVEGKAAIVVGGGQTPGQTVGNGRATAIVLGREGARVCVVD
                     RALASAQETADLIKGEGGEAWAFAADVTREDDVKRLIAAALERFGRLDILHNNVGASL
                     ALGDAVATDLTEEVFDRSVAVNLKATWLTCKHALPALREAHGSIVNIASMAALNAYPL
                     VGYKTTKAAVVAFTENLAAHNAKYGVRANSILPGLMNTPMAIESRVAAGTPREEVIAA
                     RDRRVPLGRRMGTGWDVAHAALFLHSEEAGFITGVSLIVDGGESVSWGS"
     gene            1230..2030
                     /locus_tag="VFX87_12445"
     CDS             1230..2030
                     /locus_tag="VFX87_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4995781.1"
                     /translation="MAAPALPENAYRARAERGEVQIGTWITMIRTPSILTLLQAAGLD
                     YARLDLEHSAFSIETVADMAALARALNFPLVVRPPEGNREWITRLLDAGVWNLHIPQV
                     ESAEQAAEVVAATRYAPLGSRGMYGSGPHTGYRVRPPADHMAAANARVHVTAMLESKH
                     AFDHLDAIAAVPGIDALTIGPTDLSQDLGVLGTPAQSKVLREHRVRLGEAARKHGKTV
                     AMAVDTIEAVREVIALGATIVNYSSDTNVLRSTYAAAVAEIRTPKERS"
     gene            2027..>3357
                     /locus_tag="VFX87_12450"
     CDS             2027..>3357
                     /locus_tag="VFX87_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4995782.1"
                     /translation="MKIKAVNGFARILKSEGTPWVSCYPTSPVNNALGEEGMPILMMG
                     EERFAVAVADGFSRVTNGKQIGVCTVMAGLNAAGIQMAFGAVAQAWEDSSPLLVIAEG
                     LGVGASRHTHFDMAEAFKPVTKWVGKVDRADLIPEYTRRAYTHLRSGRPGPVLLLIPR
                     DLGEYDADEHPYSPVKGWRTGPDPDDIKSAIRALLSAKDPLLHIGEGVCYADAAAELL
                     QFAEAAQIPVLTTLKAKGVFPENHPLSIGVRGSMADHFLRKCDLLFSIGSSLFPGRFS
                     HTVPDAQKKTIVQCTVDTLDINRSYETRHAVIGDAKLTLQALVQELQSKGARKKPEVL
                     EEIRGARQAFKDKFRPWLESSETPINPYRVFGDLAKVLDPKNSFLTADSGNTRDQTST
                     VYEAQIPRGYLGWGNVSTLGFSLAGAVAAKLAYPQRQCVLVTGDAGVCYMMG"
BASE COUNT          533 a         1189 c         1172 g          463 t
ORIGIN      
        1 cgcgctgtcg acccaggacc ccgagaagac cgccaagttc tacgtggacg tcttcggcat
       61 gaagcagatc ggccgggtgg accatccggc ggtcagcggc ttcttcctca ccgacgggga
      121 cctcaacctg gccgtgctca agttcaagac cgacgcggtg gccggggtcg agcggggccg
      181 agacttctcc gggatccacc acatcggctt ccaggtggac agcctcgagg agatcgcgct
      241 gcggctggcc gcggcgggcg cggcgcggcg cgacgacgtc aacgacgccc tcggggtggg
      301 ccatcaggag aagcggtacg tgaacgtgga ggtcaagtac agcggccccg acggtgtgat
      361 ggtggacgtc tccgagaccg gctgggtcgg cgcctccggc ttccgtcccc agtagcccgc
      421 tccccgatgc ccaagcgtgt cgagggcaaa gcggccatcg tcgtgggagg cggccagacc
      481 ccgggccaga cggtcggcaa tggccgggcc accgccatcg tgctgggtcg ggaaggcgcc
      541 cgggtgtgcg tggtggaccg ggcgctcgcc tcggcccagg agaccgcgga cctgatcaag
      601 ggagaaggcg gcgaggcctg ggcgttcgcg gccgacgtca cccgcgaaga cgacgtcaag
      661 cggctgatcg ccgcggcgct cgagcgcttc gggcgcctcg acatcctgca caacaacgtg
      721 ggcgcgagcc tggccctggg cgacgcggtg gccaccgatc tcaccgagga ggtcttcgac
      781 cggagcgtcg cggtcaatct gaaggccacc tggctcacct gcaagcacgc cctgcccgcc
      841 ctgcgggagg cgcacggctc gatcgtgaac atcgcgtcca tggccgcgct gaacgcctac
      901 ccgctggtgg gctacaagac caccaaggcc gcggtggtcg ccttcaccga gaacctggcc
      961 gcccacaacg cgaagtacgg tgtccgcgcc aactcgatcc tgcccgggct gatgaacacc
     1021 cccatggcga tcgagtcgcg ggtggcggcc ggcacgcctc gagaggaagt catcgccgcg
     1081 cgcgaccgcc gggtcccgct cggccggcgc atggggaccg gctgggacgt ggcccacgcg
     1141 gcgctgttcc tgcactccga ggaggccggc ttcatcaccg gggtctcgct catcgtggac
     1201 ggcggcgaga gcgtgagctg gggctcctga tggcggcgcc cgcgctgccg gagaacgcct
     1261 accgcgcgcg ggccgagcgc ggcgaggtcc agatcgggac gtggatcacg atgatccgca
     1321 ccccgtcgat cctgacgctg ctgcaggcgg ccggcctcga ctacgcccgg ctcgacctcg
     1381 agcactcggc attctccatc gagacggtgg ccgacatggc cgccctcgcg cgggcgctca
     1441 acttccctct ggtggtccgc ccgcccgagg gcaatcgcga gtggatcacg cgcctgctcg
     1501 acgccggggt gtggaatctc catattccac aggtcgagag cgccgagcag gccgcggagg
     1561 tcgtggcggc cacccgctat gcgccgctgg gctcgcgcgg catgtacggc tcgggtcccc
     1621 acaccgggta ccgcgtacgc ccgccggccg atcacatggc ggccgccaac gcccgcgtgc
     1681 acgtcaccgc gatgctcgag agcaagcacg ccttcgacca cctcgacgcg atcgcggcgg
     1741 tgccgggcat cgacgcgctc accatcggcc ccaccgacct gtcgcaggac ctgggcgtcc
     1801 tcggcacccc tgcccagtcc aaggtcctcc gcgagcaccg ggtgcgcctg ggcgaggcgg
     1861 cgcgcaagca cggcaagacg gtggcgatgg ccgtggatac gatcgaggcg gtgcgcgagg
     1921 tcatcgcgct cggcgccacc atcgtgaact actcgtccga cacgaacgtg ctgcgctcca
     1981 cgtacgcggc cgcggtcgcg gagatccgta ccccaaagga gaggtcatga agatcaaggc
     2041 ggtcaacggc ttcgcgcgca tcctcaagtc cgaaggcacc ccgtgggtga gctgctaccc
     2101 gacgagcccg gtcaacaacg cgctcggcga ggagggcatg ccgatcctca tgatgggcga
     2161 ggagcgcttc gcggtcgcgg tggccgacgg cttctcgcgc gtgaccaacg gcaagcagat
     2221 cggcgtgtgc acggtcatgg ccggcctcaa cgcggcgggc atccagatgg cgttcggcgc
     2281 cgtcgcgcag gcgtgggagg actcctcccc gctgctggtg atcgcggagg gcctgggcgt
     2341 cggggcgagc cgccacaccc acttcgacat ggccgaggcc ttcaagccgg tgaccaagtg
     2401 ggtgggcaag gtggaccgcg ccgacctgat ccccgagtac acgcgacggg cctacacgca
     2461 cctgcgctcc ggccggccgg gcccggtgct gctgctgatc ccgcgcgatc tgggcgagta
     2521 cgatgcggac gagcatccct actcgccggt gaagggctgg cgcaccgggc ccgacccgga
     2581 cgacatcaag tcggccatcc gcgcgctgct ctccgccaag gatcccctgc tgcacatcgg
     2641 cgagggcgtc tgctacgcgg acgcggccgc cgagctgctg cagttcgcgg aggcggccca
     2701 gatcccggtc ctgaccacgc tgaaggccaa gggcgtcttc cccgagaacc atccgctgtc
     2761 catcggggtg cgcggctcga tggccgacca cttcctgcgc aagtgcgacc tgctcttctc
     2821 gatcggctcg agcctcttcc ccggccgctt cagccacacg gtgccggacg cgcagaagaa
     2881 gacgatcgtc cagtgcacgg tggacacgct cgacatcaac cgcagctacg agacgcggca
     2941 cgcggtgatc ggcgacgcca agctcacgct gcaggccctg gtgcaggagc tccagagcaa
     3001 gggcgcgcgc aagaagccgg aggtgctgga ggagatccgc ggcgcgcggc aggccttcaa
     3061 ggacaagttc cgcccgtggc tcgagtccag cgagactccg atcaatccct atcgcgtctt
     3121 cggcgacctc gccaaggtgc tcgatcccaa gaactcgttc ctcaccgcgg actccggcaa
     3181 cacgcgcgac cagaccagta ccgtgtacga ggcccagatc ccgcgcggct atctcgggtg
     3241 gggcaacgtc tccacgctcg gcttcagcct ggccggcgcg gtggccgcca agctcgccta
     3301 cccgcagcgg cagtgcgtgc tcgtgacggg tgacgcgggc gtctgctaca tgatggg
//
LOCUS       DASUFA010000339         6190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1215330, whole genome shotgun sequence.
ACCESSION   DASUFA010000339 DASUFA010000000
VERSION     DASUFA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215330"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFX87_12455"
     CDS             <1..235
                     /locus_tag="VFX87_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995783.1"
                     /translation="TRMTVRLKGGRSASISPRPVPGHPANPLTLEALREKFKECAALV
                     LPADRVDTVAQIVESLDTCPDLRSLTAILDPGR"
     gene            complement(251..1030)
                     /locus_tag="VFX87_12460"
     CDS             complement(251..1030)
                     /locus_tag="VFX87_12460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX4995784.1"
                     /translation="MIATTLLVYLAAAATVFGTQFAAVIALMARRAATFDPERDGLAA
                     LLVSVPASSLALVGIAWLAAGRPRRERLRLLPARVCTPAMAAMIVGILALSQALESLA
                     LLVGVGPGPNLDWIARALAGAGPGGLLLAILVIGGLAPVGEELFFRGFMLTRLRQVWS
                     AGPAILVTAIAFGVIHGEWVHGVLATGIGIYLGLVTERSASVIPAVICHVANNTISVL
                     LSAAIGSAQGRGVNAVLLLIGAGLFAGSLRWVPPSQPEDAG"
     gene            complement(1082..2143)
                     /locus_tag="VFX87_12465"
     CDS             complement(1082..2143)
                     /locus_tag="VFX87_12465"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4995785.1"
                     /translation="MRIPRSAVVGSAAIAAVLIGMLIGFPISAAKSATAPGQGSDQKA
                     AAKGHEAGGIDPRIIERGKQTFAMYCANCHGTDGKGDGVAGQNLPIKPQNLTEGRIVN
                     ALPDHFLHSIIAHGGQEVGLSPLMPNFTPFLSDVQIREVIAYVRTLAEPAYDPTKVLP
                     VATKRQGPVQPIFFSHVIHAGSYQIACQYCHAGARRSSAAGLPSVERCMGCHKIVAAQ
                     GNPEVQKLHGYWERKEPIPWLRIFKVPEHAQFTHKPHVQAGVQCQTCHGRIEAMERVH
                     AETGQNIITDLKNLAGIPNQPPKLTMGWCVECHRTANVKGVQAVQTAADAPIPSVFPA
                     PGSESARRNAPLECVSCHH"
     gene            complement(2231..2959)
                     /locus_tag="VFX87_12470"
     CDS             complement(2231..2959)
                     /locus_tag="VFX87_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995786.1"
                     /translation="MTAAVSPILLHAERLTAGYGKMAILHDVTLDVRVGEMVSVIGPN
                     GAGKSTAFKTIVGFLRPTGGRVLFDGQDITGLRPDQVLPRGLAYVPQGRIVFPQMTVL
                     ENLEMGAYIERDGRRIQEALERVYRLFPVLAERRTQKAGTMSGGEQQMVAIGRALMTT
                     PKLILLDEPSLGLAPKFVSLIFDKLTEMKRSGYTMMVVEQNAAKALAVADRAYVLELG
                     RNRFEGSGRTLLEDPEVKRLYLGG"
     gene            complement(2956..3603)
                     /locus_tag="VFX87_12475"
     CDS             complement(2956..3603)
                     /locus_tag="VFX87_12475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4995787.1"
                     /translation="MRSDAIRSVIRAYDDPIVRAYCWGRFWILRQRFLDEIGQYLPAR
                     GRVLDLGCGFGLFSLYFASAHPALTVEGFDLNPRRIEMARAAARRLGLGNVRYEVGDV
                     MDFRGGERFDAAYMLDIVHHIPVDAVRPLLEQVAKTLPEGGRLLVKDVDRQPAYKRWF
                     THALDRVMDPRTPVRYWGAEELQPLLEEVGFEVHRHLMVDVLPYPHVLYIGRRRP"
     gene            complement(3581..5062)
                     /locus_tag="VFX87_12480"
     CDS             complement(3581..5062)
                     /locus_tag="VFX87_12480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4995788.1"
                     /translation="MRLISVASALLLAYLIWASRAWPLIHDAPLMHYIAWLIAQGAVP
                     YRDAFDMNLPGVYLLHLAVLQVGGPGDLAWRLFDLAWLAVTGALLWAYCRPVSDAVAA
                     GAGAALFGLYHLSGGAWRAGQRDFLLCLFLLAGAYGVARGLEQAGGRLALLGGGLALG
                     AGMTVKPHAGLFWLLCAAVAAAGARRAGHAAWRAGALVLGGGLVVPALVFGWLAARGG
                     LVPFVGVLTGYVLPLYSQVGRVSVLGAMRWHTWGRTSFGLFAGLGLLGLRAAARPWPP
                     RLVLAALGALYGAVHFAIQGKGWEYQLYPLALFLCALAPAAVARRGDEPLPRRVGLGA
                     APRAVALVAWAVLALVLAAKGVEAREPAWIAEKARRVDALTRDLAPLVRPGETVQVLD
                     TTAGGVHALLRMGLRQPTRFIYDFHFFHHQDDPRIGALRAELVAGLAAHPPAAVVVLE
                     ESWPDLGYARLAAFPELARALDRSFPLAVTGPGYRIHAKRRDP"
     gene            complement(5040..5744)
                     /locus_tag="VFX87_12485"
     CDS             complement(5040..5744)
                     /locus_tag="VFX87_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4995789.1"
                     /translation="MAPLLEVRSVVKRFGGVAAVNGVSLALEPGRIYGLIGPNGSGKT
                     TLFNCITGVDQADEGEILFKGRRIERMKPHQIFHLGVGRTFQVIRVFPELTALENLLV
                     VTTSGYAEAQRRARELLALVKLERLAGEFAGNLSYGQQKLVEFIRVLMTDPEMILLDE
                     PAAGVNRTLLNELLDAVRRLRDQGKTILLVEHDMKVVMGLCETVCVLDHGEKLAEGAP
                     GVIQADERVIEAYFGR"
     gene            complement(5749..>6190)
                     /locus_tag="VFX87_12490"
     CDS             complement(5749..>6190)
                     /locus_tag="VFX87_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4995790.1"
                     /translation="TVVTYVIVTSRFGLRLMTIREDEVAAEAMGIDTLRYKLYAFLLS
                     AAAPGIVGGLSARDQGYIEPLSVFPLIMTITMIVMALFGGKGTIWGPVLGAALLFAFQ
                     EVVWARFIYLHQLLFGAIIVGVVLLMPRGILGVLQQKYNLPRTI"
BASE COUNT         1009 a         2194 c         2184 g          803 t
ORIGIN      
        1 gacccgcatg accgttcgat tgaagggcgg ccggtcggcc agcatcagcc cgcggccggt
       61 gccgggccat cccgccaatc cgttgacgct cgaggcgctg cgggagaaat tcaaggagtg
      121 cgccgccctg gtgctccccg ctgatcgcgt ggacacggtg gcccagatcg tcgagtcgct
      181 cgacacctgc cccgacctcc ggagcctgac cgcgatcctc gatcccggcc gctgaccgcg
      241 cccacgcgcc tcagcccgca tcttcaggct gcgacggcgg tacccaccgc agcgagccgg
      301 cgaagagccc cgccccgatc aggagcagca ccgcgttcac gccgcgcccc tgcgccgagc
      361 cgatcgcggc cgagagcagc acggagatcg tgttgttggc cacgtggcag atcaccgccg
      421 ggatcacgct ggcggaccgc tccgtgacca gcccgagata gatcccgatg ccggtggcca
      481 gcacgccgtg cacccactca ccgtggatca ccccgaaggc gatcgcggtc accaggatgg
      541 cgggccccgc gctccacacc tggcgcagcc gcgtcagcat gaagccgcgg aagaacagct
      601 cctccccgac cggcgcgagg ccgccgatca cgaggatcgc cagcaggagc ccgccggggc
      661 cggccccggc gagggcccgc gcaatccagt cgaggttggg ccccgggccc acccccacca
      721 ggagcgcgag cgactcgagc gcctggctca gcgcgaggat ccccacgatc atcgcggcca
      781 tggccggggt gcagacccgg gctgggagca ggcgcagccg ctcgcgccgc gggcggcccg
      841 ccgcgagcca cgcgatgccg acgagggcca gggacgaggc gggcacgctg accaggaggg
      901 cggcgagccc gtcgcgctcg gggtcgaacg tggcggcgcg ccgggccatg agcgcgatca
      961 ccgccgcgaa ttgcgtgccg aagacggtcg cggcggcggc gagatagacg agaagggtcg
     1021 tagcgatcac ccacatggcc gcccgccccg actctctccc ctcaggggag agggagacca
     1081 gctagtggtg gcagctcacg cactcgaggg gggcgttgcg gcgggcactc tcgctgcccg
     1141 gggccgggaa caccgacggg atcggcgcgt ccgcagcggt ctgcaccgcc tgcacgccct
     1201 tgacgttggc ggtgcggtgg cattccacgc accagcccat ggtcagcttg ggcggctgat
     1261 tgggaatccc cgcgaggttc ttcagatcgg tgatgatgtt ctgcccggtc tcggcgtgga
     1321 cacgctccat ggcctcgata cgaccgtggc acgtctggca ctgcacgccc gcctgcacgt
     1381 ggggcttgtg ggtgaactgg gcgtgctcgg gcaccttgaa gatgcggagc cacgggatgg
     1441 gctccttgcg ctcccagtag ccgtgcagct tctggacctc gggattgccc tgggcggcca
     1501 cgatcttgtg gcagcccatg cagcgctcta ccgacggcag gcccgccgcg ctgctgcgcc
     1561 gagcgcccgc gtggcagtac tggcaggcga tctggtacga gccggcgtgg atcacgtggc
     1621 tgaagaagat cggctgcacc ggaccctgcc gcttggtggc gaccgggagc accttcgtcg
     1681 gatcgtacgc gggctcggcc agcgtccgca cgtaggcgat gacctccctg atctgcacgt
     1741 ccgacaggaa gggcgtgaag ttcggcatca gcggcgagag cccgacctcc tgccccccgt
     1801 gcgcgatgat gctgtggagg aagtggtcgg gcagcgcgtt gacgatgcgg ccttcggtca
     1861 ggttctgggg cttgatcggc aggttctggc cggcgacccc gtcgcccttg ccgtcggtcc
     1921 cgtggcagtt cgcgcagtac atcgcgaagg tctgcttgcc ccgctcgatg atccggggat
     1981 cgatgccccc ggcctcatga cccttcgccg cggccttctg gtcggagccc tggccgggcg
     2041 ctgtggccga cttggccgcg gagatgggaa acccgatcag catgccgatc agaacggccg
     2101 cgatggccgc actaccgacg acggcggaac gtgggatgcg catgaggttc gggctatctc
     2161 cctggaaaaa ccccaccgaa tatactcaaa agccggccgg aatcctagtc agaaaattca
     2221 caatctccca tcaccctcca aggtacaggc gcttgacctc ggggtcttcc agcaacgtcc
     2281 gccccgaccc ttcgaaccgg ttccggccca gctcgagcac ataggcccgg tcggccaccg
     2341 ccagggcctt ggccgcgttc tgctccacga ccatcatggt gtagcccgac cgcttcatct
     2401 cggtgagctt gtcgaagatc agggagacga acttcggggc cagacccagc gacggctcgt
     2461 ccagcaggat cagcttgggc gtggtcatga gagcccgccc gatggccacc atctgctgct
     2521 cgccgcccga catggtcccc gccttctggg tccgccgctc ggccaggacc gggaacagcc
     2581 ggtagacgcg ctcgagcgct tcctggatcc ggcggccgtc ccgctcgatg taggcgccca
     2641 tctcgagatt ctccagcacc gtcatctggg ggaacacgat gcggccctgg gggacatagg
     2701 ccaggccgcg cggcagcacc tggtcgggcc ggaggcccgt gatgtcctgc ccatcgaaga
     2761 ggacccgccc gccggtcggg cgcagaaagc ccacgatggt cttgaaggcc gtggacttgc
     2821 ccgcaccgtt ggggccgatg acgctcacca tctcgcccac gcgcacgtcg agcgtgacgt
     2881 cgtggaggat ggccatcttg ccatacccgg cggtgagacg ctcggcgtgc agcaggatcg
     2941 ggctgactgc ggcggtcaag gccgacgccg cccgatgtag agcacgtggg gatagggcag
     3001 gacgtccacc atcaggtgcc ggtgcacctc gaagcccacc tcctccagga gcggctgcag
     3061 ctcctccgcg ccccagtagc gaacgggcgt ccggggatcc atgacccggt ccagggcgtg
     3121 ggtgaaccag cgcttgtagg ccggctggcg atccacgtcc ttgacgagca ggcgcccgcc
     3181 ctcgggcagc gtcttggcca cctgctcgag caggggccgc accgcgtcca ccggaatgtg
     3241 gtggacgatg tcgagcatgt aggcggcgtc gaagcgctcg ccgccccgga aatccatgac
     3301 gtcgccgacc tcgtaccgga cgttgccgag accgagccgc cgcgcggccg cgcgggccat
     3361 ctcgatgcgg cgtggattga gatcgaagcc ttcgacggtg agcgccggat gcgcgctcgc
     3421 gaagtagagc gagaagagcc cgaagccgca gcccaggtcg agcacccgac cccgcgcggg
     3481 aaggtactgg ccgatctcgt cgagaaatcg ctggcgcagg atccagaagc gcccccagca
     3541 gtacgcgcgc acgatcggat cgtcgtaggc gcggatgacg ctacggatcg cgtcgcttcg
     3601 catggatccg gtatccgggg ccggtgacgg ccagggggaa ggagcggtcg agcgcccgcg
     3661 ccagctcggg gaacgcggcc agccgcgcgt agccgaggtc gggccagctc tcctcgagga
     3721 ccaccaccgc ggcgggcggg tgggccgcga ggcccgccac cagctccgcg cgcagggcgc
     3781 cgatgcgcgg gtcgtcctgg tggtggaaga agtggaagtc gtagatgaag cgcgtgggct
     3841 gccggagacc catgcgcagg agcgcatgga cgccccccgc ggtggtgtcg agcacctgca
     3901 cggtctcgcc cgggcgcacc agcggggcca ggtcgcgggt cagcgcgtct acccgccgcg
     3961 ccttctccgc gatccaggcg ggctcgcgcg cctcgacgcc cttggcggcc agcacgagcg
     4021 cgagcaccgc ccaggccacg agcgcgaccg cgcggggcgc ggcgcccaga ccgacccgcc
     4081 gcggaagcgg ctcgtccccg cggcgcgcca ccgccgcggg cgccagcgcg cagaggaaga
     4141 gcgcgagcgg gtagagctga tactcccagc ccttgccctg gatcgcgaag tgcacggcgc
     4201 cgtagagcgc gcccagggcc gccagcacga ggcgcggcgg ccagggccgg gcggcggcgc
     4261 gcaggcccag caggccgagc cccgcgaaca ggccaaagct cgtgcgcccc caggtatgcc
     4321 agcgcatggc cccgagcacc gacacgcggc cgacctggct gtagagcggc agcacgtagc
     4381 cggtgagcac gcccacgaac ggtaccaggc cgccgcgtgc ggcgagccag ccgaacacga
     4441 gcgcgggcac caccagcccg ccgcccagca cgagcgcgcc ggcgcgccag gccgcgtgcc
     4501 cggcgcggcg agcgcccgcc gcggccacgg ccgcgcacag cagccagaag agacccgcgt
     4561 gtggcttcac ggtcatgccc gcgccgaggg cgagcccgcc ccccaggagg gccaggcgcc
     4621 cgcccgcctg ctcgaggccc cgcgccacgc cgtaggcgcc cgcgagcagg aagaggcaga
     4681 gcaggaaatc gcgctgccct gcgcgccacg cgccgccgct cagatgatag aggccgaaca
     4741 gggctgcgcc cgcgccggcc gcgaccgcgt cgctcaccgg tcggcagtag gcccagagga
     4801 gcgcaccggt gacggcgagc caggccaggt cgaagagccg ccatgccaga tcgccgggac
     4861 cgcccacctg gagcacggcc aggtgcagga gatagacgcc gggcaggttc atgtcgaagg
     4921 cgtcgcgata cgggaccgcg ccctgcgcga tgagccaggc gatgtagtgc atcaggggcg
     4981 cgtcgtggat caggggccag gcgcgcgagg cccagatcag gtacgcgagc agcagcgcgc
     5041 tagcgaccga aataagcctc aatgacccgc tcgtcggcct ggatgacgcc gggcgccccc
     5101 tcggccagct tttcgccgtg atcgagcaca cacaccgtct cgcagaggcc catgaccacc
     5161 ttcatgtcgt gctcgaccag caggatggtc ttgccctggt cgcgcaggcg cctcaccgcg
     5221 tcgagcagct cgttcagcag ggtgcggttg acgccggcgg cgggctcgtc gagcaggatc
     5281 atctcgggat cggtcatgag cacgcggatg aactccacga gcttctgctg cccgtaggag
     5341 agattgcccg cgaactcacc cgcgagccgc tcgagcttca cgagcgcgag cagctcccgg
     5401 gcccggcgct gcgcctccgc gtacccgctc gtcgtcacca ccagcaggtt ctcgagggcg
     5461 gtcagctctg ggaagacccg gatcacctgg aaggtgcggc ccactccgag atggaagatc
     5521 tggtgcggct tcatgcgctc gatgcggcgg cccttgaaga ggatctcccc ctcgtcggcc
     5581 tggtctaccc cggtgatgca gttgaacagc gtggttttgc ccgagccgtt cggcccgatg
     5641 agcccgtaga tgcgaccggg ctcgagcgcg agcgagacgc cgttgaccgc ggcgacgccg
     5701 ccgaagcgct tcaccaccga acggacctcg aggaggggcg ccacggggct agatggtccg
     5761 cggcaggttg tacttctgct ggagcacgcc caggatcccg cggggcatca ggagcaccac
     5821 ccccacgatg atcgcgccga agaggagctg gtggaggtag atgaagcgcg cccacaccac
     5881 ctcctggaag gcgaagagca gcgcggcccc gagcaccggc ccccagatcg tgcccttgcc
     5941 gccgaacagc gccatcacga tcatcgtgat ggtcatgatg agcggaaaca ccgagagggg
     6001 ctcgatgtag ccctggtcgc gcgcggagag gccgcccacg atcccgggag ccgccgcgga
     6061 gagcaggaag gcgtagagct tgtagcgcag ggtgtcgatc cccatcgcct ccgcggccac
     6121 ctcgtcctcg cggatggtca tgagccgcag cccgaagcgg gaggtgacga tgacgtaggt
     6181 gaccacggtc
//
LOCUS       DASUFA010000340         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1578360, whole genome shotgun sequence.
ACCESSION   DASUFA010000340 DASUFA010000000
VERSION     DASUFA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578360"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFX87_12495"
     CDS             <1..417
                     /locus_tag="VFX87_12495"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX4995791.1"
                     /translation="RTNMVTALQRAGGALNTNVHFHALVLDGVLTEDQEGALTFHPAP
                     EPSNAEVAGALAMIRHRVQRLLVRHGLEPADEATGPADRFADESPVLAEIVGASVQGR
                     VALGQRAGARVRRIVDTRDTAAVSSRGPRQAHLDGF"
     gene            complement(550..1824)
                     /locus_tag="VFX87_12500"
     CDS             complement(550..1824)
                     /locus_tag="VFX87_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4995792.1"
                     /translation="MTTAAPQALEAYDRGVEAALGWKANALDLFREATRLDPSLAVAH
                     AGAAACLFLEERFGETRAAGEAAQAAATSGVSPRERSYVNAVTLWTAGKVPEAEAAMR
                     EHLAQYPRDLAIIQRLYFVFFWRGKFPAMLEATSELVRHHPESSYMLGMHAFALEEAG
                     RCADAIRLAEEAIAKEPEDAWSIHALAHALYESAAFDTGVTRLPAAIEPCRGLNWFQN
                     HLWWHLVLMHLSRGEYARVSDMSRRLFEQMPSSIAGDLHDSISLLWRLELTGNPPGDR
                     WQPFTAIARDRIARTGLLFHVAHVAMALVGGGDWTSAEKQLALLRDRAPKDPTGLMGE
                     VTVPLVEGLHAFAARDWPRVIAKIEPLRPRIVEFGGSRAQRDVFHDTLIEACFRGGDM
                     DRAERLLAERLHRRPDHFWLNRRRAGAVPSPS"
     gene            complement(2025..2651)
                     /locus_tag="VFX87_12505"
     CDS             complement(2025..2651)
                     /locus_tag="VFX87_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995793.1"
                     /translation="MYRAATIALSLGLGALLITPPLLAQSPPAAPAPAHQMGHHMHGQ
                     GHDAAEGAAGAPTQPGQDAFGAIAEVVRLLEADSETDWSRVDLERLRQHLIDMNEVVL
                     RSEVKSSQVPGGLAMDITGPPRTARAIRAMVEPHAVELDGMSEWAARTAPIPGGLRLT
                     VTARTPEDAKTVARIRGLGFVGLLVQGGHHGPHHLAMAKGAALVGHRH"
     gene            complement(2729..>3406)
                     /locus_tag="VFX87_12510"
     CDS             complement(2729..>3406)
                     /locus_tag="VFX87_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4995794.1"
                     /translation="AADIILLEKRLAVLHDGVVEGRRSFGNIMKYVLMGTSSNFGNML
                     SMAAASLFLLFLPMLPLQILLNNFLYDLAQVPIPSDRVDGTYMRKPKRWNIGFIRRYM
                     LLTGPLSSVFDFATFAVMLWVFEAGPALFRTGWFVESLATQTLVIFVIRTDGPPWRSR
                     PSRGLVWGVSTCATAGALLPFTPLAPWLGFTPLPPLFFGFLLVMVTAYLGLMEVVKRW
                     FYRRYPM"
BASE COUNT          516 a         1189 c         1229 g          472 t
ORIGIN      
        1 cggaccaaca tggtcacggc gctgcagcgc gcgggcgggg cgttaaacac gaatgtccac
       61 tttcatgccc tcgtgctgga cggggtgctc accgaagacc aggagggcgc gctgacgttt
      121 cacccggcgc cggagccgag caacgccgag gtcgcggggg cgctggcgat gatccgccac
      181 cgggtgcagc ggctgctggt gcgccacggc ctggagccgg ccgacgaggc gaccgggccg
      241 gcggatcggt tcgcggacga gtctcccgtg ctggcggaga tcgtgggcgc ctcggtccag
      301 gggcgggtgg cactgggcca acgcgcgggg gcacgggtgc ggcggattgt cgacacgcgc
      361 gacacggcgg ccgtgtcatc gcgcgggccg cggcaggcgc acctggacgg cttctgacgc
      421 atcaagctct ccttgtgatc tgcgttgccc cgcgcgaacg tcgtccgccc ccgggagccg
      481 tcgccacagc gtcttcctcc ccgcgaagcg gcggagcggc tggggccggc gtgccccagt
      541 gggtgtggct cagctgggcg agggcaccgc gccggcccga cgccggttga gccagaagtg
      601 gtcggggcgc cggtggaggc gttcggcgag caggcgctcg gcgcgatcca tgtcgccgcc
      661 gcggaagcac gcctcgatca gggtgtcgtg gaagacgtcg cgctgggcgc ggctgccgcc
      721 gaactcgacg atccgcgggc gcagcggctc gatcttcgcg atcacgcgcg gccagtcgcg
      781 ggccgcgaag gcgtggagcc cctccaccag cggcaccgtc acctcgccca tcagccccgt
      841 cggatccttc ggcgcccgat cgcgcaggag ggcgagctgc ttctcggcgc tcgtccagtc
      901 gccgccaccc accagcgcca tcgccacgtg cgcgacgtgg aacagcaggc cggtccgcgc
      961 gatgcggtcg cgcgcaatgg cggtgaacgg ctgccagcgg tcgcccggcg gattcccggt
     1021 cagctcgagt cgccacagga gcgagatcga gtcgtgcagg tcgcccgcga tcgaggaggg
     1081 catctgctcg aacaggcgcc gcgacatgtc cgacacccgg gcgtactcgc cgcgcgagag
     1141 gtgcatgagc accagatgcc accagaggtg gttctggaac cagttgaggc cgcggcacgg
     1201 ctcgatcgcg gccggcagcc gggtcacccc ggtatcgaac gcggccgact cgtagagcgc
     1261 gtgggcgagg gcgtgaatcg accacgcgtc ctcgggctcc ttcgcgatgg cctcctccgc
     1321 gagccggatg gcgtccgcgc accggcccgc ctcctccagc gcgaaggcgt gcatgcccag
     1381 catgtaggag ctctcgggat ggtgacggac cagctcgctg gtcgcctcca gcatggcggg
     1441 gaacttcccg cgccagaaga agacgaagta gagccgctgg atgatcgcga ggtcgcgcgg
     1501 atactgcgcg aggtgctcgc gcatcgccgc ttccgcctcg ggcaccttgc cggccgtcca
     1561 cagggtgacc gcattgacgt agctgcgctc gcgagggctc acgcccgacg tcgcggcggc
     1621 ctgcgccgcc tcgccggccg cgcgggtttc gccgaaccgc tcttccagga acaggcaggc
     1681 cgccgccccc gcgtgggcca ccgccagtga cggatcgagc cgcgtggcct cgcggaacag
     1741 gtcgagagcg ttcgccttcc atccgagcgc ggcctcgacg ccgcggtcgt aggcctcgag
     1801 cgcctgcggg gccgccgtcg tcaccggtag accgtatcga tcgcttggca tgccgggatt
     1861 ctaccgcaac cggagacggg ccagtatcgc gtcatgcgcc agaccgttcg taacgatcag
     1921 cgcgtgccgg ttcgcgagat tgggcccgcg atagtcgatc ggcgccccac acgcgtccgc
     1981 tcgtgaccac tctcgattcc ggccggcgga cggggaaggc cgcgtcagtg ccggtggccc
     2041 accagcgccg ctcccttggc catggccagg tggtgcgggc cgtgatggcc accctgcacc
     2101 aggagcccga cgaagcccag gccgcggatc cgcgcgaccg tcttggcgtc ctcgggcgtg
     2161 cgcgccgtca ccgtgaggcg gaggccgccc ggaatcggcg cggtcctggc ggcccactcg
     2221 gacatgccgt ccagctcgac cgcgtgcggc tcgaccatgg cacggatggc ccgcgcggtc
     2281 ctcggcgggc cggtgatgtc catggccagg ccgcccggaa cctggctcga cttgacctcg
     2341 gagcgcagga cgacctcgtt catgtcgatg aggtgctggc gcagccgctc gagatcgacc
     2401 cggctccagt cggtctcgga atccgcctcc agcaggcgga ccacctcggc gatcgcgccg
     2461 aaggcgtcct ggccgggctg cgtcggcgcc ccggccgccc cctcggccgc atcgtgaccc
     2521 tggccgtgca tgtgatggcc catctggtgc gccggcgcgg gggcggcggg cgggctctga
     2581 gcaaggagcg gcggtgtgat cagcaaggct ccgaggccga ggctcagcgc aatcgtggcg
     2641 gctctgtaca tggtgtcctc ccgggttgga tcgagcgcct ccagcatagg caccgccggg
     2701 cgcttctgtc cgtgacttgg gtcacgcttc acatcggata gcgtcggtag aaccagcgct
     2761 tcaccacctc catcaggccc agatacgcgg tcaccatgac gagcaggaac ccgaagaaga
     2821 gcggcggcag cggcgtgaag ccaagccagg gcgccagggg cgtgaacggc aagagagccc
     2881 ccgcggtcgc gcaggtgctc acgccccata ccagcccgcg gctggggcgg ctgcgccagg
     2941 gcggcccgtc cgtccggatc acgaagatca ccagggtctg cgtggccagc gactccacga
     3001 accatcccgt ccggaagagc gccggccccg cctcgaacac ccagagcatc accgcgaacg
     3061 tggcgaaatc gaacaccgac gacagcggac cggtcagcag catgtaccgc cggatgaagc
     3121 cgatgttcca ccgcttgggc ttgcgcatgt aggtgccgtc cacacgatcg gacgggatcg
     3181 gcacctgggc gagatcgtac aggaagttgt tgagcaggat ctgcagcggc agcatcggga
     3241 ggaagagcag gaagagcgag gccgccgcca tgctcagcat gttgccgaag ttggagctgg
     3301 tgcccatcag aacgtacttc atgatgttgc cgaagctccg ccggccctcg acgacgccat
     3361 cgtggagcac cgcgagtcgc ttctcgagca agatgatgtc ggcggc
//
LOCUS       DASUFA010000341         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_519149, whole genome shotgun sequence.
ACCESSION   DASUFA010000341 DASUFA010000000
VERSION     DASUFA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519149"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VFX87_12515"
     CDS             complement(<1..1139)
                     /locus_tag="VFX87_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4995795.1"
                     /translation="MVGFASEMGEFGVGQSVKRFEDVRLLRGEGRFHADVNLPGQVHA
                     VLVRSPHAHARIRAIDTAPALQAPGVLAVYTGADVARDGLGTMKMTLKRARPDGSPMF
                     APPHRGLALDRARHVGDAVAMVVAETRAQAEDAAELVRVDYEPLPSVTDTAAAAEPGA
                     PAVWDECPDNISNLYPAGDAAATDAAFARAHRVVRRRYVITRVHAQFMEARGALGVYD
                     PGEDRYTLYADVQYPHRVRNALATNIFKIPEHQIRVIAGDVGGAFGTKGWQYVEHRLV
                     LWAARKLGRPVKWLCERREAIPADEHARDNVTDAELALDAQGRFLALRVRTLANVGAY
                     VSSDRNLLATFSNVATLVGVYTFPAALVEVRCMLTNTNPTAPYRGA"
     gene            1222..2733
                     /locus_tag="VFX87_12520"
     CDS             1222..2733
                     /locus_tag="VFX87_12520"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX4995796.1"
                     /translation="MPVWLALALVGCLVGSAQAARPESLEVRDRGASLGLLTITVMDD
                     GSAYIAPDHLAALLKGAWAVKGERGTLTVGNRIAEFTRGQTRAIVHGQPIALDVPPRV
                     SGREWLVSRDFLGKALPRLAPGVSLALPTEPRKPAAKTVQAAVPLEELRYRSYPSFTR
                     IVIETGARLAYAVVTGDEEVRVRLPGLSLAHSRVEEISDGLVQEVRLEPAGDTAILRV
                     VLQGTAGEVKHTVLQDPYRLVLDIHRPRESGGAGEPGRGAMPPLRLIVMDAGHGGHDA
                     GAVGPTGVQEKDVVLDVTRRVARKVEAGLGVKVVLSRDSDVFVPLRDRTNFANKQRAD
                     LFVSIHANAHPRSVSEGVETYFLSSEASDSGARQVAAIENGVVQLENPGSRQRGDVLK
                     SILWDLAQSEFQEESSSMAETVLDSMSKSLNLVNRGVKQAGFYVLGGAAMPAILIEIG
                     FLTNRKEERKLATPEHREAMARAIYAGLAEYKRRYDQRLRTAQSQPAKGGPTR"
     gene            2742..3476
                     /gene="rph"
                     /locus_tag="VFX87_12525"
     CDS             2742..3476
                     /gene="rph"
                     /locus_tag="VFX87_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX4995797.1"
                     /translation="MRHDGRQPDQLRPVTITRDYLRHPEGSVLVEFGDTKVLCTASLE
                     EKVPPFLKGQGKGWVTAEYGMLPRSTNTRMARERSGPSGRSQEIQRLVGRSLRSVVEM
                     AKLGERTVWVDCDVIQADGGTRTAAITGAFVALGDAIGTLVKAGTLAGSPIRDCVAAI
                     SVGMVGNRPVLDLNYVEDSTAEVDMNVVMTGAGAFVEVQGTAEQTPFSKARLDELLTL
                     AGDGIGRLIGLQRRALEARGDKRFTL"
     gene            3523..>3850
                     /locus_tag="VFX87_12530"
     CDS             3523..>3850
                     /locus_tag="VFX87_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX4995798.1"
                     /translation="MAALLADLPYRIQDLAEFPGVVLPPEGDTSYAENALGKARAVTA
                     ATGLLALADDSGIEVDAFDGRPGVLSARYGGEGLTDPERNALMLRELQGVPAARRTAR
                     YRAVIAV"
BASE COUNT          545 a         1356 c         1419 g          530 t
ORIGIN      
        1 gcgccgcggt agggcgcggt ggggttggtg ttggtcagca tgcagcggac ctcgacgagg
       61 gcggccggga acgtgtagac gccgacgagg gtcgccacgt tgctgaacgt ggcgagcagg
      121 ttgcggtcgg aggagacata ggcgcccacg ttggcgaggg tgcgcacgcg cagggcgagg
      181 aagcggccct gggcgtcgag cgccagctcg gcgtcggtca cgttgtcgcg cgcgtgctcg
      241 tcggccggga tcgcctcgcg ccgctcgcac agccacttca ccggccggcc gagcttgcgc
      301 gcggcccaca gcacgagccg gtgctcgacg tactgccagc ccttggtccc gaaggcgccc
      361 cccacgtcgc ccgcgatgac ccggatctgg tgttccggga tcttgaagat gttggtcgcg
      421 agcgcgttgc gcacgcgatg cgggtactgc acgtcggcgt agagcgtgta gcgatcctcg
      481 cccgggtcgt agacgccgag ggcgccgcgc gcttccatga actgcgcgtg cacgcgggtg
      541 atcacgtagc ggcgccggac cacgcggtgg gcgcgcgcga aggccgcgtc ggtggccgcg
      601 gcgtcgcccg ccgggtacag attggagatg ttgtccgggc actcgtccca gaccgcggga
      661 gccccgggct cggcggccgc cgcggtgtcg gtcaccgacg ggagcggctc gtagtcgacg
      721 cgcacgagct cggccgcgtc ctcggcctgc gcgcgggtct ccgccaccac catcgccacc
      781 gcatcaccca cgtgacgcgc acggtcgagc gcgagcccgc ggtgcggcgg cgcgaacatc
      841 ggcgagccgt cgggccgggc gcgcttgagg gtcatcttca tggtaccgag gccgtcgcgc
      901 gccacgtcgg cgccggtgta gaccgcgagc acccccgggg cctggagggc gggggcggtg
      961 tcgatcgcgc ggatgcgcgc gtgggcgtgg ggggagcgca ccagcaccgc gtggacctgg
     1021 ccgggcaggt tcacgtcggc atgaaagcgc ccctcgcccc gcagcagtcg aacgtcctcg
     1081 aatcgcttga cagactgccc gacgccgaac tcgcccatct cggaggcgaa gcctaccaca
     1141 aattgatcct tcgccacctc ctcgctccgt tgctagaata gcttcgctgc gcccccttgg
     1201 atcatgatgc gacgactctc gatgcccgtc tggttggccc tggctctggt gggctgcctc
     1261 gtcggctcgg cgcaggccgc gcgcccggag tccctggagg tgcgcgaccg gggcgccagc
     1321 ctcgggctcc tgaccatcac cgtcatggat gacggcagcg cgtacatcgc ccccgaccac
     1381 ctggctgccc tcctcaaggg cgcatgggcg gtgaaggggg agcgcggcac cctgaccgtg
     1441 ggcaaccgca tagccgagtt cacccgcggt cagacgcgcg ccatcgtgca cggccagccc
     1501 atcgcgctcg acgtgccccc gcgcgtgagc gggcgggaat ggctggtctc acgtgatttc
     1561 ctgggcaagg ccctgccacg gctggcgccg ggggtctcgc tcgccttgcc gaccgagccg
     1621 cggaagccag cggccaagac ggtgcaggcc gcggtgccgc tcgaggagct gcgctaccgc
     1681 tcctatccct cgttcacgcg catcgtcatc gagaccggcg cgcggctggc ctatgcggtg
     1741 gtcaccggcg acgaggaggt gcgggtgcgg ctacccggcc tgtccctcgc ccactcccgt
     1801 gtcgaggaga tcagcgacgg gctcgtgcag gaggtccgcc tcgagcccgc gggcgacacc
     1861 gcgatcctgc gcgtggtgct ccagggcacc gcgggcgagg tgaagcacac cgtgctccag
     1921 gacccgtacc ggctcgtgct cgacatccac cggccccggg agagcggcgg cgcgggcgag
     1981 ccggggcgcg gtgcgatgcc cccgctccgg ctgatcgtga tggacgccgg ccacggcggc
     2041 cacgacgcgg gcgcggtggg cccgaccggg gtgcaggaga aggacgtggt gctggacgtg
     2101 acgcgccggg tggccaggaa ggtcgaggcg gggctgggcg tgaaggtcgt gctgagccgc
     2161 gacagcgacg tgttcgtccc gctgcgtgac cgcaccaact tcgccaacaa gcagcgcgcc
     2221 gacctcttcg tgtccatcca cgccaacgcg catccccggt cggtgtcgga gggcgtcgag
     2281 acgtatttcc tctcctccga ggcctccgac agcggcgcgc gacaggtggc ggccatcgag
     2341 aacggcgtgg tccagctcga gaacccgggc tcgcgccagc gcggggacgt gctcaagagc
     2401 atcctctggg acctggccca gtcggagttc caggaggagt cgagctccat ggccgagacg
     2461 gtgctggact cgatgagcaa gtcgctcaac ctggtcaatc ggggcgtcaa gcaggccggg
     2521 ttctacgtgc tgggcggggc ggccatgccg gccatcctca tcgagattgg attcctcacg
     2581 aaccggaagg aggaacggaa gctggccacg ccggagcacc gcgaggccat ggcgcgcgcg
     2641 atctacgccg ggctcgccga gtacaagcgc cgctacgatc agcgcctgcg caccgcgcag
     2701 agccagccgg ccaagggcgg gccgacccga tgaccaccca tctgcgccac gacggccggc
     2761 agccggacca gctccggccg gtcacgatca cgcgggacta cctgcgtcac cccgagggct
     2821 ccgtcctcgt cgagttcggc gacaccaagg tgctctgcac cgcctcgctc gaggagaagg
     2881 tgcccccctt cctcaagggg caggggaagg gctgggtcac cgcggagtac gggatgctgc
     2941 cgcgctccac caatacccgc atggcgcgcg agcggagcgg tccgagcggg cgctcccagg
     3001 agatccagcg gctggtcggc cggtcgctgc gctcggttgt cgagatggcc aagctcggcg
     3061 agcgtacggt gtgggtggac tgcgacgtca tccaggccga cggcggcacc cgcaccgccg
     3121 cgatcaccgg cgccttcgtg gcgctgggcg atgccatcgg caccctcgtg aaggcgggaa
     3181 cgctggccgg ctcgccgatc cgggactgcg tggccgcgat cagcgtgggc atggtgggca
     3241 accggcccgt gctcgacctc aactacgtcg aggattccac cgccgaggtg gacatgaacg
     3301 tggtgatgac cggcgcgggg gccttcgtgg aggtgcaggg cacggccgag cagacgccgt
     3361 tctcgaaagc gcgcctcgac gagctgctga ccctggccgg cgacggcatc ggccgattga
     3421 tcggcctgca gcgccgcgcc ctcgaggcgc gtggcgacaa gcgcttcacc ctctgaccgg
     3481 cggctggtcc tggccacggc caaccgggcc aagggccggg agatggccgc tctcctggcc
     3541 gacctgccct atcgcatcca ggacctcgcc gagtttcccg gcgtcgtcct gcccccggaa
     3601 ggcgacacct cctatgccga gaacgcgctc ggcaaggcgc gcgccgtcac cgcggcgacc
     3661 gggctgctgg cgctggccga cgactcgggc atcgaggtgg acgccttcga tgggcggccg
     3721 ggggtcctct ccgcccgcta cggcggcgag gggctgaccg atcccgagcg caacgccctc
     3781 atgctgcggg agctccaggg cgtccccgcc gcccggcgca cggcccgcta ccgcgcggtg
     3841 atcgcggtga
//
LOCUS       DASUFA010000342         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1216489, whole genome shotgun sequence.
ACCESSION   DASUFA010000342 DASUFA010000000
VERSION     DASUFA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216489"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFX87_12535"
     CDS             complement(<1..436)
                     /locus_tag="VFX87_12535"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4995799.1"
                     /translation="MADVLDVLIVGGGAAGYTAGIFAARDRCRALLLERFSSGGQVLN
                     CEHITNFPGFPQGVAGYALGPLLQEQATAAGLQVAMHEVTAVRRDGDLLLVETDGGSH
                     ATRTLIVASGSRFTTLGIPGEEEFVGRGISHCASCDGSFFMQK"
     gene            complement(452..955)
                     /locus_tag="VFX87_12540"
     CDS             complement(452..955)
                     /locus_tag="VFX87_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4995800.1"
                     /translation="MAIKVGDRVPDGTLTEFIEQETPGCTVGPNAFQVADLTKGKKIV
                     VFGLPGAFTPTCSAKHVPSYVANIDKLKSKGVDEVWCVSVNDAFVMGAWAKDQKSSGK
                     VRMMADGSAALTKAMGLEMDLTARGMGVRSQRYSMLVDNGVVKSLNVEQPGKFEVSDA
                     DSMLKQL"
     gene            1154..1486
                     /locus_tag="VFX87_12545"
     CDS             1154..1486
                     /locus_tag="VFX87_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4995801.1"
                     /translation="MKPKLRVWVTFGEDLKFGDGRARLLALIDERGSLKKAAQELEMS
                     YRNAWGYLRDLEEAAGFKFVERVPGGGPESGMRLTKAGKRFLERYHKFRSGLDEAARR
                     QFDRAFGA"
     gene            1483..1830
                     /locus_tag="VFX87_12550"
     CDS             1483..1830
                     /locus_tag="VFX87_12550"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX4995802.1"
                     /translation="MSPTAKYLYLTTLGRRSGLARQIEIWFTRHAGRYYLVAEHGLKA
                     HWVRNLLAEPAVRARVGRRSFRGRARVVDARSERDLVAAIRARSEAKYGWGTGLVVEI
                     APAGRRRRGAPAR"
     gene            complement(1827..>2909)
                     /gene="selD"
                     /locus_tag="VFX87_12555"
     CDS             complement(1827..>2909)
                     /gene="selD"
                     /locus_tag="VFX87_12555"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX4995803.1"
                     /translation="AVTPEFAAAPMRGGDMLCGGCAAKVGESSLTRALERLGVPSHPA
                     VVLGLAQPDDAAAVETERGEIVAATVDSFRAFAADPYLVGRVAAVNAVSDLWAKGVVP
                     RFALAQVSVPEAEPARAEETLYQVMAGARAALEADGVTLVGGHTTTGPELVVGFAVWG
                     FAASAGALIRIGGAAAGDQLLLTKPLGTGVILQADMRGLARGEWVEAAAASMLRSNAS
                     AARAMVALRPSAATDVTGFGLAGHLGEMLRASKGSAVLDLAAIPALPGAVALLGRGIR
                     STAHPENARARRAMLVEPHAARQPSLDLLFDPQTSGGLLVTVPRERAPAMLRALCAAG
                     DGAAAIIGEVAPPRPDGALIRVVAAG"
BASE COUNT          393 a         1055 c         1071 g          390 t
ORIGIN      
        1 gcttctgcat gaagaacgat ccgtcgcagg aggcgcagtg ggagatgccg cgccccacga
       61 actcctcctc gccggggatc ccgagcgtgg tgaagcgcga gccgctcgcg acgatcaggg
      121 tgcgcgtcgc gtgggagccg ccgtcggtct ccaccaggag gagatcgccg tcgcggcgga
      181 ccgcggtgac ctcgtgcatc gcgacctgga ggccggccgc ggtcgcctgc tcctggagca
      241 gcgggcccag cgcgtaaccc gcgaccccct ggggaaagcc cgggaagttg gtgatgtgct
      301 cgcagttgag cacctggccc ccggaggaga acctctcgag cagcagggcg cggcagcgat
      361 cgcgggcggc gaagatgccg gcggtgtagc cggcggctcc gccgcccacg atcaggacgt
      421 ccaggacgtc cgccatgcgc tcggtcgtgg cctacagctg cttcaacatc gagtcggcgt
      481 cgctcacctc gaacttgccg ggctgctcga cgttcagcga cttcaccacg ccgttgtcca
      541 ccagcatcga gtagcgctgc gagcgcaccc ccatgccgcg ggcggtgaga tccatctcga
      601 ggcccatcgc cttggtgagg gcggcgctgc cgtcggccat catccggacc ttgccggagc
      661 tcttctgatc cttggcccac gcgcccatga cgaaggcgtc gttgaccgac acgcaccaca
      721 cctcgtcgac gcccttgctc ttgagcttgt cgatgttggc cacgtagctc ggcacgtgct
      781 tggccgaaca ggtgggggtg aaggcgcccg gcaggccgaa gacgacgatc ttcttgccct
      841 tggtcaggtc cgccacctgg aacgcgttgg gacccacggt gcagcccggg gtctcctgct
      901 cgatgaactc cgtgagggtg ccgtccggca cgcggtcacc gactttgatc gccatgagtg
      961 tctcctcctc gagggtggtt tgagcgcggc ggcccgctcg ctcgattcag cgggcggcgg
     1021 ctagggtacc acagccggcc ctggcctgcc cggacgcggg tatccgtcac ccggcgcgcg
     1081 gcggagcctg ggtgcccgga agatcgtcgc ctgcctggtg tgcgggcggg ccttccgagt
     1141 aagatgcgcc ctgatgaagc ccaagctgcg ggtgtgggtc acgttcggcg aggacctcaa
     1201 gttcggggac gggcgcgccc gtctcctcgc gctgatcgac gagcgcggct cgctcaagaa
     1261 ggccgcccag gagctcgaga tgtcctaccg caacgcgtgg ggctacctgc gcgatctcga
     1321 ggaggcggcc ggcttcaagt tcgtcgagcg ggtgccgggg ggcggccccg agagcggcat
     1381 gcggctcacg aaggccggca agcgcttcct cgaacgctac cacaagttcc ggagcggcct
     1441 cgacgaggcg gcccggcgac agttcgaccg cgccttcggc gcgtgagccc cacggcgaag
     1501 tatctctacc tgaccacgct cggtcggcgc agcgggctgg cccgccagat cgagatctgg
     1561 ttcacccgcc acgcgggccg ctactacctg gtcgccgagc acggcctgaa ggcgcactgg
     1621 gtgcgcaacc tcctcgcaga gccggcggtg cgcgcgcggg tcggccgccg gtccttccgt
     1681 ggccgcgccc gcgtggtgga tgcgcggtcg gagcgcgatc tcgtcgccgc catccgggcg
     1741 cggtcggagg ccaagtacgg ctggggcacc ggcctcgtcg tcgagatcgc tccggcgggg
     1801 cggcgccggc gcggcgcgcc cgctcgctag cccgccgcca ccacccggat cagggcgccg
     1861 tcgggtcggg gcggcgcgac ctcgccgatg atcgcggcgg ctccgtcgcc ggcggcgcac
     1921 agcgcacgca gcatcgccgg ggcacgctcc cggggcaccg tgaccaacag cccccccgag
     1981 gtctggggat cgaagagcag gtcgagcgaa ggctgccgcg ccgcgtgcgg ctccacgagc
     2041 atggcgcgcc gcgcgcgggc gttctcggga tgcgcggtgc tccggatgcc gcggcccagc
     2101 agcgccaccg cgccgggcag ggccgggatt gcggcgaggt cgaggacggc cgagcccttg
     2161 ctcgcgcgca gcatctcgcc caggtgcccg gcgaggccga agccggtgac gtcggtggcg
     2221 gccgacggcc gcagcgcgac catcgcgcgg gcggccgagg cgttggagcg gagcatcgag
     2281 gccgcggccg cctccaccca ttcaccccgc gcgaggccgc gcatgtcggc ctgcaggatc
     2341 acgccggtgc cgagcggctt ggtcaggagg agctggtctc cggccgccgc cccgccgatg
     2401 cggatcagcg cgcccgccga ggccgcgaag ccccagaccg cgaagcccac gaccagctcg
     2461 ggccccgtgg tggtgtggcc gcccacgagc gtgacgccgt cggcctcgag cgcggcccgg
     2521 gcgcccgcca tcacctggta cagcgtctct tccgcccgcg ccggctcggc ttccggcacg
     2581 ctcacctggg ccagggcgaa gcgtggcacc acgcccttgg cccagagatc gctgaccgcg
     2641 ttgaccgcgg ccacgcgccc gaccaggtag ggatcggcgg cgaaggcgcg gaaggagtcg
     2701 acggtggcgg cgacgatctc gccgcgctcg gtctccaccg cggccgcgtc atcgggctgg
     2761 gccaggccca ggacgacggc gggatggctc gggaccccga ggcgctcgag cgcgcgggtc
     2821 agcgaggatt cgcccacctt ggccgcgcac ccgccgcaga gcatgtcacc gccgcgcatc
     2881 ggggccgccg cgaactccgg cgtgacggc
//
LOCUS       DASUFA010000343        15327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_761153, whole genome shotgun sequence.
ACCESSION   DASUFA010000343 DASUFA010000000
VERSION     DASUFA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 15327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15327
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761153"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="recA"
                     /locus_tag="VFX87_12560"
     CDS             <1..817
                     /gene="recA"
                     /locus_tag="VFX87_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX4995804.1"
                     /translation="LALHIIAEAQHMGGTAAFIDAEHALDPKYAKNLGVNTDELLISQ
                     PDTGEQALEIAEALVRSNAVDVTVIDSVAALVPRAELDGDMGDSLPGLQARLMSQALR
                     KLTAAISRSGGAVIFINQIREKIGVMFGSPETTTGGRALKFYSSIRLDIRRQDTIKNG
                     TEAIGVRTKVKVVKNKLSPPFREAEFDVIYGEGISKEGSVLDLAAEHNVVEKSGTWYT
                     YKSERIGQGRENAKRYLKDNPKLLADLEVKVRDALGLKPVGGVSQPAPEKVAK"
     gene            831..2033
                     /locus_tag="VFX87_12565"
     CDS             831..2033
                     /locus_tag="VFX87_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX4995805.1"
                     /translation="MELQQLLTETAQRKASDLHLKPGSKPILRIHGHLEVQDDLPQIT
                     REFIDAMAASLLGERRLNMLMEGREMDLGYMLPGVGRFRCNLFLAMGEVRGVFRHVPE
                     RIPAFHELHLPKVLERLSMERRGMILVTGITGSGKSTSLASMIDFMNRSRNDHIVTIE
                     DPIEFVHQDKKCVLSQREIGHDSVNFAQALRAALREDPDIMLVGELRDVETMEVGLHA
                     AETGHLVLSTLHTLNAAETINRMISMFPPHQEDQIRGQLAAVIQGIVSQRLVVRADGR
                     GRVPAVEVMIATGLIRDCIREADKTPQIPSVIAAGTAQYGMQTFDQSLLQLYREELVT
                     YETARDAATNPDDFDLKVKGIFSTGEMTWEHPGGSNTASTAAPTQPGMAAATPATTAR
                     SPFGKKAG"
     gene            2126..2560
                     /locus_tag="VFX87_12570"
     CDS             2126..2560
                     /locus_tag="VFX87_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX4995806.1"
                     /translation="MTRRLRRRGAPAEVAQSVVADLIDRGYVDDQAFARWWAEARARG
                     RRVGSHRLRQELLAKGIPRAMAEAAITAAFEEVGESERALEAGRRRLPALLRGQAARA
                     PARLADYLLRRGYPAAAVRGAVRVLLAERGADLSGIEEDGGA"
     gene            2613..5291
                     /gene="alaS"
                     /locus_tag="VFX87_12575"
     CDS             2613..5291
                     /gene="alaS"
                     /locus_tag="VFX87_12575"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX4995807.1"
                     /translation="MTGSEIRARFLEYFRRRGHTVVRSSPLVPAQDPTLLFTNAGMVQ
                     FKSVFLGEERRDYVRAATAQKCVRAGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFK
                     EDAIRFCWELLTEDFGLPKDRLKATVFTDDDDAFELWKKVAGLGPDRILRLGEKDNFW
                     AMGDTGPCGPCSEVHFHQGDHLLCAEEQAGRTCLGPACDCDRWLEIWNLVFMQFNRDA
                     SGTMTPLPTPSIDTGMGLERLAAVLQGKQSNFETDLLRPLIAHVERLCGKTYGRDEEA
                     DVSMRVIADHARAATFLITDGVTPSNEWRGYVLRRIMRRAMRHGRMLGLEAPFLWDVT
                     GSVVTVMGDAYPEIRDAQARVAETVRQEEERFAETLDLGMAKIREYLEAHKADTNRVV
                     DGRFLFTLYDTHGFPSDLAQEVLQDAGFTLTPETQKTFDAEMEAQRERARAGASFGAG
                     DSGEPSVAIYQRLSTELAKPQFLGYGSLAAPARILALVADGQRRREARQGETVEIILD
                     RTPAYAESGGQQGDAGSVIGREGQGQILDTYYRGSQLIVHRVAVTQGQLREGEEVAVS
                     VESRRRQGLRQHHTGTHLLHAALRRVLGTHVAQAGSLVAPDHLRFDFSHGTAVKDSEV
                     EEVESLVNEQVQANIVVSPAEMDLQEALRSGAMALFGEKYGDRVRVIRIGDFSTELCG
                     GTHLDATGQIGLFKVTDEGAVASGVRRIEAVTGEHALAHVGREEAALRESAGLLKIPP
                     LELPRRLAALLEDQKQLEKQLAQLETKLARSQAQDLAAAAKQVAGVPVLAARLDGLDP
                     DGLRAVVDTLRDRLPSGIILLGSAVDGKVSLVAAVSKDLMKRFPAGRLVQEIAKMVGG
                     GGGGRPDLAQAGGKDASKLDEALGAVAGWVERTAQA"
     gene            5345..6046
                     /locus_tag="VFX87_12580"
     CDS             5345..6046
                     /locus_tag="VFX87_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995808.1"
                     /translation="MSESRGKTLVVDDELDVAQMLTEYLTEQGYQVVTAHGGVEALTK
                     IDLEKPQVILLDIRMPGMDGVEVLRRIRSFDTQVGILMISANDDVDLAKQTIAMGAFD
                     YTLKPVDFAYLSRAVDTMMARSPAQAPPHAEGAGAATPSVQNLLYDLALAIFRTARTF
                     SPMARESLGRELEQAALVALQRGAGGEKQEVVRALNQIRTLLRFAKDLGDITDDAHRG
                     LEASVARARRSIGLS"
     gene            6083..8083
                     /locus_tag="VFX87_12585"
     CDS             6083..8083
                     /locus_tag="VFX87_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4995809.1"
                     /translation="MCPMNCHPTLCGMLVTVEDGRLVEVAGDPDNPDSRGFLCVRGHA
                     SREIIGNPQRLLTPLARDRRDTPFREVTWDAALDRIASRMRAVGREAVGLWSGHGFFA
                     NNYGTRVNSELLRRFANLWGCQWWAPTMICWGLGGFGIGLTGPLETNTKEDMGAHAEL
                     VLLWGANLASQPNTAPHLIAARRRGAHIVTIDVRRTEAAAQSDEVLIIRPGTDAALAL
                     GMMHVLIAENRWDRDFVARHTVGFDALAAHVRRYPPAWAAAVTGLDEAVIVSLARRYA
                     ATRPAMIVLGGSSMHKGANGWQGARAVACLPALTGNLGIAGGGLGPRHGSKGHGFGLT
                     DIARAERRPPGDYVPNQMPRITEALETGRVRALLLFGTDMLSSFADSGRLEAALARQE
                     LIVSHELFWNDTARRFADVVLPGTAWLEELGCKATNTHLYLMEPALPPAGQARTGAWV
                     LRELARRLEVADFYPWTDAAGPIDAILDHPATGHATVAALRVEGGMRALRVSHVGHPD
                     LAFPTPSGKVELYSARAESLGLAPLPGHEDLPVSRFPLVLRHGRTLAHFHGFYDHGRA
                     LPSLAALEPEPVLWLSPADAAARAVADGAAVRVFNERGEMRARARVTDRVPAHTVWMR
                     DGWDGLNRLTSGDACIPDAAVDVFGFSAGQAAFDAAVEVSPV"
     gene            7992..8759
                     /locus_tag="VFX87_12590"
     CDS             7992..8759
                     /locus_tag="VFX87_12590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4995810.1"
                     /translation="MRASRTPRWTSSASRRARPRSTRRWRSRLSERPRALLFDFGGTL
                     DADGVTWKERFFALWRAEGAADRPEAFDPVFYRADDALVDAIPETLSFEETVRRLTAN
                     IASGLAVRDERLIARVADRFLDDAHRHLRRNQALLTRLGARYRLGLVSNFYGNLETVC
                     HNTAILSLFGVIVDSVRAGFSKPDPRIFMRALDGLGVAPLDATFVGDSVARDMAGARA
                     LGMRHIWLIADPPPPGGPCCPGDAMIHSLTDVEALLL"
     gene            8759..9463
                     /locus_tag="VFX87_12595"
     CDS             8759..9463
                     /locus_tag="VFX87_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX4995811.1"
                     /translation="MADSVRGAIIAAGEGSRLRADGYRVPKPMVEVAGLPLIGAVLRN
                     FTTAGITAITIILNEQERECVQWVRTRFPALEIDFIVKTTASSLESFREVTGRGPAGP
                     VLVSTVDAWCAAEDFARFAAAAASRPDDATVLAVTPFVSDEKPLWVTLGAGGRVEGLG
                     TGDSGLVTAGVYRVSDRARRLAPTRELGRLREYLGWLHESGEPLYGEIIETVVDVDRA
                     LDVASAEALARGVRRT"
     gene            9460..10335
                     /locus_tag="VFX87_12600"
     CDS             9460..10335
                     /locus_tag="VFX87_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995812.1"
                     /translation="MSAKQTGGADLTCWGLFREREHSPGRESDDAEILRLTGKHLETY
                     GVQVELRAPEDLVATEVPPDGVFFMCERMEALGTLLAWQERGVRLVNSPLAVLNTYRE
                     RMIAQLREANVAFVPSRLVTTGDADVAARLPVWVKRADVHNTQEGDVVFAETPDAVAA
                     ALAGLAARGIERAVLQPHVAGDLIKFYGVGPSGGGPDGGPPWFRWFYHKDQRLAGHAF
                     DPVRLARLVREAATALGLEIYGGDAIASADGSLQLLDLNAWPSFALYRDEAAPVIASY
                     LALRFGARRASGGGR"
     gene            10332..11660
                     /locus_tag="VFX87_12605"
     CDS             10332..11660
                     /locus_tag="VFX87_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4995813.1"
                     /translation="MSEPREAGPRSREIVAREARHIAPGYQSFALYSGLAMARGSGAT
                     LWDEDGNEYIDFIAGIAVGSVGHCHPHYVEALKRQAERLTFGSFTTETRARFLELMAS
                     VTPEGLTRIQLFSGGAEAVEAALRLAKAATGRQEVVGFWGGFHGKTGGVLGLLGSDFK
                     HHQGPFLPGQYSTPYADCYRCPLKLRYPDCGIACAEYVRDVIRYQTAGEIAAIIVEPI
                     QGTAGNIIPPDGFLRAIQSVARDHGALLLADEMITGFGRTGSMWGTDHEGVVPDIMTV
                     GKGVGGGFPLAGVVSTEKLTSAKPWSNPSFSSSSYGGNPLAAAAGLAALEIILKEDLV
                     EQSNRVGAVMLAELQRLKEKYRTVGEVRGRGLLLGIELVEDRQSRKPLDKSITRDLYL
                     ECLRRGLVAMTYAPTIRLNPPLVITEETARAGLAILDEALGVTLKRHGRD"
     gene            11647..12690
                     /locus_tag="VFX87_12610"
     CDS             11647..12690
                     /locus_tag="VFX87_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4995814.1"
                     /translation="MGETRRLRGAVIGLGNVAVHGHLPGWRQRPDAVIVAATDLHPAR
                     QAECRAALPGARWHDSVDALLAADDLDFVDVCTPPSSHAALIEAALAHGLHVLCEKPL
                     VASLAELERLVGLARTARAVLHTVHNWHQAPLVRATDALIREGRIGAVREVVWRTLRT
                     QPAAAAGGDSWRVDPAIAGGGVLTDHGWHVSYVVPRWVGAPPDAVSARLETRRHHRFA
                     VEDTATVRLQFPDAVADIVLTWAAETRENQAHVIGTAGRIDIEDDTVVLRVDGREERH
                     RRPPALSDGSQHPDWFGPVTDEFIARVLGQDPSSRNLDEAAVCIAVEGGARESSRRGG
                     VMFPLAARVGSAG"
     gene            12687..13856
                     /locus_tag="VFX87_12615"
     CDS             12687..13856
                     /locus_tag="VFX87_12615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4995815.1"
                     /translation="MTTLFSTAVSVRSLLVILPAPPGAGSPPPGTVIAGLPLVRRIVL
                     AAERAGFERILVHPRACPEPRLLAGTAAALLDADTPVARRARLVLVPVNVLPQARWLR
                     ALREMPLDPETLAVDSSLTAVVESAGIDEVLRSAGQAAGAEDLIPALSRRLARATEAS
                     DPAGRLTLGDASGPQAAEDWLLRSLIKDNEGFMSRHVERRISLAITRRLVNTRITPNE
                     MTLVSLTIGLLAAPFFLSAEPLQQIVGSLLFLTHSILDGCDGELARLKFQESPIGARL
                     DFWGDNLVHVAVFGCMAGGWARAEASLWPLLLGALAITGTAASAYLVAHRDGGAAATG
                     PPGRLADAVANRDFIYLVLVLACFGRAHWFLIPVALGTPIFMMLVRWGAGRRRPT"
     gene            13858..>15327
                     /locus_tag="VFX87_12620"
     CDS             13858..>15327
                     /locus_tag="VFX87_12620"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX4995816.1"
                     /translation="MFSVLDVLLRGFILAAQATAVGGVCFLLLVARGPEAPRSLGLIA
                     AAAATLALGQALSLANELFVLLHEAVWPLRAALDTLYVQVSVVRIAAGLALAACAFGL
                     RRAGGPSRLWPAVVALAAVLVVAAPWTSHAAARLDSRILLLLLDGAHQLGAAVWIGGL
                     VHLTAAAFRRGDRPWPVALLRRFSTLALSAVAVLVGAGVGLTWNYAPDQTAIFGTAYG
                     LMVLTKLAILGGLLVLGALNTRAIRRMDGAREADQLTLRRFVEVEVGLGLTVLFAAAS
                     LTSLPPAVDLTHDRATLAEVATRLTPRMPSLRSPSISEMPIDDPLAPRNDADRAWSEF
                     NHHVSGILVLIMGLLALLHATGRARWARHWPLVFLGLAAFMIVRNDPGSWPLGPEGFW
                     EGWLVATVAQHRVFVLLVVVFGIFEWMVRSGRLASRRAALVFPLLCVVGGGLLLSHSH
                     AAQNLKDEFLLEVSHAPLGLLALLIGWGRWLELRLPEGRG"
BASE COUNT         2114 a         5479 c         5486 g         2248 t
ORIGIN      
        1 gctggcgctc cacatcatcg cggaggcgca gcacatgggc ggcaccgccg ccttcatcga
       61 cgccgagcac gcgctcgatc ccaagtacgc caagaacctc ggagtcaaca ccgacgagct
      121 gctcatctcc cagcccgata ccggcgagca ggcgctcgag atcgcggagg ccctcgtgcg
      181 ctccaacgcc gtggacgtca ccgtgatcga ctcggtggcc gcgctcgtgc cgcgcgcgga
      241 gctggatggc gacatgggcg actcgctccc gggtcttcag gcgcgcctca tgtcccaggc
      301 cctgcgcaag ctcaccgctg cgatctcccg atctggtgga gcggtgatct tcatcaacca
      361 aatacgtgaa aagattggcg tcatgttcgg atcgccggag acgaccaccg gcgggcgggc
      421 gctgaagttc tactcctcta tacggctgga catccggcgg caggacacga tcaagaacgg
      481 caccgaggcc atcggggtcc ggaccaaggt caaagtggtg aagaacaagc tttctccgcc
      541 cttccgcgag gccgaattcg acgtcatcta cggcgagggg atctccaagg aggggtcggt
      601 cctggacctg gccgccgagc acaatgttgt cgaaaaatcc ggcacttggt acacctataa
      661 gagtgagcga atcgggcagg gacgggagaa cgccaagcgg tacctgaagg acaatcccaa
      721 gctcttggcc gacctggaag tcaaagtccg cgatgccctt ggcctcaagc cggtaggggg
      781 ggtgtcgcag ccggcgccgg agaaggtggc caagtaggag cgggtacacc atggaactgc
      841 agcagctgct gacggagacc gcgcagcgca aggcttcgga cctgcacctg aagccgggtt
      901 cgaagccgat cctcaggatc catggtcacc tcgaggtgca ggacgacctg ccgcagatca
      961 cccgcgagtt catcgacgcg atggcggcga gcctcctcgg agagcgtcgt ctcaacatgt
     1021 tgatggaagg ccgggagatg gacctgggct acatgctgcc cggggtcggc cgcttccgct
     1081 gcaacctctt ccttgccatg ggcgaggtgc gcggcgtctt ccggcacgtg ccggagcgca
     1141 tccccgcctt ccacgagctg cacctgccca aggtcctcga gcggctctcg atggagcgcc
     1201 gcgggatgat cctggtcacc gggatcaccg gctccggcaa gtccaccagc ctcgcgtcca
     1261 tgatcgactt catgaaccgg agccggaacg accacatcgt cacgatcgag gacccgatcg
     1321 agttcgtgca ccaggacaag aagtgcgtgc tgagccagcg cgagatcggc cacgactcgg
     1381 tgaacttcgc ccaggccctc cgcgccgccc tccgcgaaga ccccgacatc atgctggtcg
     1441 gtgagctgcg ggacgtggag accatggaag tgggcctcca cgccgcggag accggccacc
     1501 tcgtgctctc aacgctccac accctgaacg cggcggagac catcaaccgg atgatctcca
     1561 tgttcccgcc gcaccaggag gaccagatcc ggggccagct cgccgcggtc atccagggca
     1621 tcgtctcgca gcgcctcgtg gtgcgcgccg atggccgcgg ccgcgtgccc gcggtcgagg
     1681 tcatgatcgc gaccggtctc atccgcgact gcatccgcga ggccgacaag accccgcaga
     1741 tcccgagcgt catcgcggcg ggcacggcgc agtacggcat gcagaccttc gaccagtcgc
     1801 tcctgcagct ctatcgcgag gagctggtca cgtacgaaac cgcccgcgac gccgcgacga
     1861 acccggacga cttcgacctc aaggtcaagg gcatcttctc caccggcgag atgacctggg
     1921 agcatccggg cggtagcaac accgcctcga ccgccgcgcc gacgcagccg ggcatggctg
     1981 ccgccacacc ggccacgact gcgcgcagcc ccttcggcaa gaaagcgggg tagcactgcc
     2041 gccgcccgca ccgacgctgg acgcgaaggc cgccagactg gcggccttcg atcttctggc
     2101 ccgcaagtcc tggagcggcc gcgagctgac ccgtcgcctc cggcgccggg gcgcccccgc
     2161 cgaggttgcc cagtccgtgg tggccgatct catcgaccgc ggctacgtgg acgatcaggc
     2221 cttcgcccgc tggtgggccg aggcccgcgc ccgtgggcgc cgggtcggca gccaccggct
     2281 gcggcaggag ctcctcgcca aagggattcc ccgggccatg gcggaagccg cgatcaccgc
     2341 cgcgttcgaa gaggtggggg agtccgagcg cgcgctcgag gccgggcgcc gccgcctgcc
     2401 cgcgctgctg cgcggccagg ccgcccgggc gcccgcccgg ctggccgact acctcctgcg
     2461 ccgcggttat ccggccgcgg cggtgcgtgg cgcggtgcga gtactactgg ccgagcgggg
     2521 ggccgacctc tccggcatcg aggaagacgg cggcgcataa ttccgggcgc ccgattccgg
     2581 gcgtctgaac ccgggcgtat aataccggtc tgatgaccgg cagcgagatc cgcgcgcgat
     2641 tcctcgaata cttccgccgg cgcgggcata cggtggtgcg gtcctccccg ctggtgcccg
     2701 cccaggatcc caccctgctc ttcaccaacg cgggcatggt gcagttcaag tcggtgttcc
     2761 tgggggagga gcgccgggac tacgtccggg cggccaccgc ccagaagtgc gtgcgggccg
     2821 gcggcaagca caacgacctg gagaacgtcg gccgtaccgc ccggcaccac accttcttcg
     2881 agatgctggg caacttctcc ttcggggact acttcaaaga ggacgcgatc cggttctgct
     2941 gggagctcct gaccgaggac ttcggcctcc ccaaggaccg gctcaaggcc accgttttca
     3001 ccgacgacga cgatgccttc gagctgtgga agaaggtggc cgggctcggc ccggatcgca
     3061 tcctccgcct gggcgagaag gacaacttct gggcgatggg ggacaccggc ccgtgcggtc
     3121 cctgctccga ggtgcacttc caccagggtg atcacctgct ctgcgcggag gagcaggcgg
     3181 gacggacctg ccttggcccg gcctgcgact gcgatcgctg gctcgagatc tggaacctgg
     3241 tcttcatgca gttcaaccgg gacgcgagcg gcacgatgac gccgctcccc acgccctcga
     3301 tcgacaccgg catggggctc gagcggctcg ccgcggtgct ccagggcaag cagtccaact
     3361 tcgagaccga cctgctccgg ccgctcatcg cccacgtcga gcgcctctgc ggcaagacct
     3421 acggccgcga cgaggaggcg gacgtgtcga tgcgggtcat cgcggaccac gcgcgcgcgg
     3481 ccaccttcct gatcacggac ggagtgacgc cgtccaacga gtggcgtggc tacgtgctcc
     3541 ggcggatcat gcgccgggcc atgcgtcacg gccgcatgct ggggctcgag gcgcccttcc
     3601 tgtgggacgt caccggcagc gtggtcacgg tgatgggcga cgcttacccg gaaatccgcg
     3661 acgcgcaggc gcgggtggcc gagacggtgc gccaggaaga ggagcgcttc gcggagacgc
     3721 tcgatctcgg catggccaag atccgcgagt acctggaggc ccacaaggcc gatacgaacc
     3781 gggtcgtgga cggccgcttc ctgttcacgc tctacgacac ccacggcttt ccctccgacc
     3841 tggcgcagga ggtgctccag gacgcgggat tcacgctcac gcccgagacc cagaagacct
     3901 tcgacgccga gatggaggcg cagcgcgagc gcgcccgcgc cggggcctcg ttcggggcgg
     3961 gggacagcgg cgagccgagc gtcgccatct accagcggct ctccaccgag ctggccaagc
     4021 cccagttcct gggctatgga tcgctcgccg cgcccgcgcg gatcctcgcc ctggtggcgg
     4081 acggccagcg ccgccgcgag gcgcgccagg gtgagacggt cgagatcatc ctggaccgca
     4141 cgcccgccta cgcggagtcc ggcggccagc agggcgacgc cggctcggtg atcgggcgcg
     4201 agggccaggg gcagatcctg gacacctact accgcggcag ccagctcatc gtgcaccggg
     4261 tggcggtgac ccagggacag cttcgcgagg gtgaggaggt cgcggtcagc gtggagtcgc
     4321 ggcggcggca ggggctcagg cagcaccaca ccggcacgca tctgctccac gccgcgctgc
     4381 ggagggtgct gggcacccac gtggcgcagg cgggctcgct ggtggcgccg gaccacctgc
     4441 gcttcgactt ctcgcacggc accgccgtca aggacagcga ggtggaggag gtcgagagcc
     4501 tggtcaacga gcaggtgcag gccaacatcg tggtgagccc ggccgagatg gacctgcagg
     4561 aggcgctccg cagcggggcc atggcgctgt tcggcgagaa gtacggcgat cgggtgcgcg
     4621 tcatccgcat cggcgacttc tccaccgagc tgtgcggcgg cacccacctc gacgcgaccg
     4681 gccagatcgg cctcttcaag gtgaccgacg agggcgcggt ggcctccggc gtgcgccgca
     4741 tcgaggcggt caccggcgag cacgcgctcg cccacgtggg ccgcgaggag gccgcgctgc
     4801 gggagtccgc gggcctgctc aagatcccgc cgctggagct gccccgccgc ctcgccgcgt
     4861 tgctggagga tcagaagcag ctcgagaagc agctcgccca gctcgagacc aagctcgcgc
     4921 gcagccaggc gcaggacctg gcggccgccg cgaagcaggt ggcgggcgtg ccggtgctcg
     4981 ccgcgcggct cgacgggctc gacccggacg ggctgcgcgc ggtggtggac acgctgcggg
     5041 accggctgcc gtcggggatc atcctgctcg gcagcgcggt ggacggcaag gtgagcctcg
     5101 tcgcggccgt ctccaaggac ctgatgaagc gcttccccgc gggccgtctc gtccaggaga
     5161 ttgccaagat ggtgggcggc ggcggtggcg gccgcccgga cctggcccag gccggtggca
     5221 aggacgcctc gaagctcgac gaggccctcg gcgcggtggc gggctgggtg gagcggacgg
     5281 cgcaggccta gcccggccca ctggcgcgcc ggccggatcc gcgtaccagg aaggagccca
     5341 aggcatgtcc gaatcccgtg gcaagaccct cgtggtcgac gatgagctcg acgtggcgca
     5401 gatgctcacc gagtacctga ccgagcaggg gtaccaggtc gtgacggccc acggcggcgt
     5461 cgaagcgctg accaagatcg atctcgagaa gccgcaggtg atcctgctcg atatccggat
     5521 gcccgggatg gatggggtcg aggtcctgcg ccggatacgg agcttcgaca cgcaggtggg
     5581 tatcctgatg atcagcgcca atgacgacgt cgatctggcc aagcagacca tcgcgatggg
     5641 ggctttcgac tacacgctga aaccggtcga cttcgcctat ctgagccgcg ccgtggacac
     5701 gatgatggcc cgctcaccgg cccaggcgcc gccccatgcg gagggcgcgg gtgccgcgac
     5761 gccctccgtg cagaaccttc tctacgatct cgcgctcgcc atcttccgga ccgcgcggac
     5821 cttctcgccg atggcgcgcg agtcgctggg acgcgagctc gagcaggccg cgctggtcgc
     5881 gctgcagcgg ggcgccggtg gggagaagca ggaggtcgtc cgcgcgctca atcagatccg
     5941 gaccctcctt cgcttcgcga aggacctggg cgacatcacc gacgatgccc accgggggct
     6001 cgaggcaagc gtggcccggg cgcgccggtc catcggcctg tcataggtcg agatgcggtc
     6061 accgcgccgg accatccgca ccatgtgccc gatgaactgc catcccactc tctgcgggat
     6121 gctcgtcacc gtcgaggacg ggcggctcgt cgaggtcgcg ggcgacccgg acaatcccga
     6181 cagccggggc ttcctctgcg tgcgcggcca cgcttcgcgc gagatcatcg gcaacccgca
     6241 gcgcctgctg accccgctcg cgcgcgaccg gcgcgacacg ccgttccggg aagtgacgtg
     6301 ggacgccgcg ctcgatcgca tcgcgtcgcg catgcgggcg gtcgggcgcg aagcggtcgg
     6361 gctctggtcc ggccacggct tcttcgccaa caactacggc acccgcgtca actcggagct
     6421 gctccgccgc ttcgccaatc tctggggttg tcagtggtgg gcgcctacca tgatctgctg
     6481 ggggctcggc ggcttcggga tcgggctcac cggcccgctc gagacgaata ccaaggagga
     6541 catgggggcc cacgccgagc tcgtcctgct gtggggcgcg aacctcgcga gtcagccgaa
     6601 cacggccccg catctgatcg cggcgcgccg tcggggcgcc catatcgtca ccatcgacgt
     6661 gcggcgcacc gaggcggccg cgcagtcgga cgaggtgctg atcatccggc ccggcaccga
     6721 cgcggcgctg gccctcggca tgatgcacgt gctgatcgcg gagaatcgct gggaccgcga
     6781 cttcgtcgcg cggcacaccg tcgggttcga cgccctcgcc gcgcacgtgc ggcggtaccc
     6841 gcccgcgtgg gccgccgcgg tcaccggcct cgacgaggcg gtcatcgtga gcctggcccg
     6901 gcgctacgcg gccacgcggc cggccatgat cgtgctgggc ggcagcagca tgcacaaggg
     6961 cgccaacggc tggcagggcg cgcgcgcggt ggcgtgcctg cccgcgctca ccggcaacct
     7021 gggcatcgcg ggcggcggcc tgggcccgcg ccacggcagc aagggccacg gcttcggcct
     7081 caccgacatc gcgcgggccg agcggcggcc gcccggcgac tacgtgccga accagatgcc
     7141 gcggatcacc gaggcgctcg agaccggccg ggtgcgcgca ctgctcctct tcggcaccga
     7201 catgctatcg tcgttcgcgg acagcggacg cctggaagcc gcgctggcgc gccaggagct
     7261 gatcgtcagc cacgagctct tctggaacga caccgcgcgc cgcttcgcgg acgtcgtgct
     7321 gcccggcacc gcgtggctcg aggagctcgg ctgcaaggcg accaacacgc atctctacct
     7381 gatggagccg gcccttccgc ccgccgggca ggcccgcacc ggcgcgtggg tgctgcgcga
     7441 gctcgcgcgg cgcctcgagg tcgccgactt ctacccgtgg acggacgcgg cggggcccat
     7501 cgacgcgatc ctcgatcacc ccgcgaccgg acacgccacg gtggcggccc tgcgcgtgga
     7561 gggcgggatg cgtgcactcc gcgtctcgca cgtgggccat cccgatctgg catttcctac
     7621 tccttccggg aaggtggagc tctattcggc gcgcgcggag agcctcggcc tggctccgct
     7681 gccggggcac gaggacctgc ccgtctcgcg cttcccgctc gtgctccgcc acgggcggac
     7741 cctcgcgcat ttccacggct tctatgatca cggccgcgcg ctgccgtcgc tggccgcgct
     7801 cgagcccgag ccggtgctgt ggctctcgcc cgccgacgcg gccgcgcgcg cggtggcgga
     7861 cggcgccgcg gtgcgcgtgt tcaacgagcg cggcgagatg cgcgcccgcg cgcgggtgac
     7921 ggaccgcgtg cccgcgcata ccgtctggat gcgcgacggg tgggacggcc tcaaccggct
     7981 cacctccggg gatgcgtgca tcccggacgc cgcggtggac gtcttcggct tctcggcggg
     8041 ccaggccgcg ttcgacgcgg cggtggaggt ctcgcctgtc tgagcgtccc cgcgcactgc
     8101 tcttcgactt cgggggcacg ctcgacgccg acggggtgac gtggaaggag cgattcttcg
     8161 ccctctggcg cgccgagggc gcggcggatc gccccgaggc cttcgatccc gtcttctacc
     8221 gcgccgacga cgcgctcgtg gacgcgatcc cggagaccct ctccttcgag gagacggtga
     8281 ggcggctcac cgccaacatc gcctccggcc tcgcggtgcg cgacgagcgg ctgatcgcgc
     8341 gcgtggccga ccgcttcctc gacgacgccc accgccatct gcgacggaac caggcgctgc
     8401 tcacgcggct cggcgcgcgc taccggctcg gtctcgtgtc caacttctat gggaacctcg
     8461 aaaccgtgtg tcataatacg gcgatcctgt ctctcttcgg cgtcatcgtc gactccgtgc
     8521 gggccgggtt ctccaagccg gatccgcgca tcttcatgcg ggcactggac gggctcgggg
     8581 tggccccgct cgacgccacg ttcgtgggcg attcggtggc ccgggacatg gcgggcgcgc
     8641 gggcgctcgg catgcggcac atctggctga tcgccgaccc gccgccgccg ggcgggccgt
     8701 gctgcccggg cgacgcgatg atccattcac tgacggacgt ggaggctttg ctgctctgat
     8761 ggcggattcg gtgcgaggcg cgatcatcgc ggccggtgag ggcagccgtc tacgcgcgga
     8821 cggctaccgg gtgccgaagc cgatggtgga ggtggccggg ttgccgctga tcggcgcggt
     8881 gttgcgcaat ttcacgaccg ccggcatcac cgcgatcacc atcatactga atgagcagga
     8941 gcgcgagtgc gtccaatggg tccggacgcg gttccccgcc ctcgagatcg acttcatcgt
     9001 gaagaccacc gcgtcctcgc tcgagagctt ccgggaggtg accgggcgcg ggcccgcggg
     9061 gccggtgctg gtgtccacgg tggacgcgtg gtgcgcggcg gaagacttcg cgcgcttcgc
     9121 ggcggccgcg gcttcccggc ccgacgacgc caccgtgctc gcggtcaccc cgttcgtctc
     9181 ggacgagaag ccgctctggg tgacgctggg ggcgggtggg cgcgtggaag gcctcggtac
     9241 gggggatagc ggtctggtca ccgcgggggt gtatcgtgtt tcagatcggg cgcggcggct
     9301 cgcgcccacg cgggagctcg gccggctgcg cgagtacctg gggtggctcc acgagagcgg
     9361 cgagccgctc tacggggaga tcatcgagac cgtggtggac gtggaccggg cactggatgt
     9421 ggccagcgcg gaggcgctgg cgcgaggagt tcgacggaca tgagcgcgaa gcagactggg
     9481 ggggcggatc tgacgtgctg gggactcttt cgcgagcgtg agcactcgcc gggacgggag
     9541 agcgacgacg cggagatcct gcgattgacc ggcaagcatc tggagacgta cggcgtccag
     9601 gtggagctcc gggcccccga ggatctcgtc gccaccgagg tgccgcccga cggcgtgttc
     9661 ttcatgtgcg agcgcatgga agcgctcggc acgctgctgg cttggcagga gcggggcgtg
     9721 cggctcgtca actcgccgct ggccgtgctc aacacctacc gggagcggat gatcgcgcag
     9781 ctccgcgagg ccaacgtggc cttcgtgccg agccggctcg tgaccacggg cgacgcggac
     9841 gtggcggccc gactaccggt ctgggtcaag cgcgccgacg tgcacaacac gcaggaaggc
     9901 gacgtggtct tcgcggagac gccggacgcc gtcgccgccg cgctcgccgg gctcgcggcc
     9961 cgcggcatcg agcgcgcggt gctgcaaccg catgtggccg gcgacctcat caagttctac
    10021 ggagtcggcc ccagcggcgg cggcccggac ggagggccgc cctggttccg ctggttctac
    10081 cacaaggacc agcgccttgc cggccatgcc ttcgacccgg tccggctggc gcgactcgtg
    10141 cgcgaggctg ccaccgcgct cggcctcgag atctacgggg gcgacgccat cgcctccgcg
    10201 gacggctcgc tccagctcct cgatctcaac gcgtggccca gcttcgcgct ctaccgcgac
    10261 gaggccgcgc cggtgatcgc gtcgtacctg gccctgcgct tcggcgcgcg gcgggcctcc
    10321 ggaggcgggc ggtgagcgag ccgcgcgagg ccgggccccg ctcgcgggag atcgtggcgc
    10381 gcgaggcgcg ccacatcgct cccgggtatc agtccttcgc gctctactcg gggctcgcca
    10441 tggcccgcgg cagcggcgcg actctgtggg acgaggacgg caacgagtac atcgacttca
    10501 tcgcggggat cgcggtgggg agcgtgggcc actgccaccc gcactacgtc gaggccctga
    10561 agcgtcaggc cgagcgcctg accttcggca gcttcaccac cgagacgcgc gcgcgcttcc
    10621 tcgagctgat ggcttccgtc actccggaag ggctcacgcg catccagctc ttctcgggcg
    10681 gcgcggaggc ggtggaggcg gccctgcgcc tggccaaggc cgccaccggg cggcaggagg
    10741 tcgtcggctt ctggggtggc ttccacggca agaccggcgg cgtgctgggc ctgctcggca
    10801 gcgatttcaa gcatcatcag ggtccgttcc tgcccggcca gtactcgacg ccgtacgccg
    10861 actgctaccg ctgcccgctc aagctccgct atccggactg cggcatcgcc tgcgcggagt
    10921 acgtccgcga cgtcatccgc taccagaccg cgggcgagat cgccgcgatc atcgtggaac
    10981 cgatccaggg caccgcgggg aacatcatcc cgcccgacgg cttcctgcgg gcgatccagt
    11041 ccgtggcccg cgaccatggg gcgttgctcc tcgccgacga gatgatcacc ggcttcggcc
    11101 gcaccgggtc gatgtggggc accgaccacg agggcgtcgt gcccgacatc atgacggtgg
    11161 gcaagggcgt gggcggcggc ttcccgctgg ccggcgtggt ctccaccgag aagctgacga
    11221 gcgccaagcc ctggtccaat ccgagcttca gctcgtcgag ctacggcggc aacccgctcg
    11281 ccgcggcggc ggggctcgcc gcgctcgaga tcatcctgaa ggaggatctc gtcgagcagt
    11341 ccaatcgcgt cggcgcggtc atgctcgccg agctccagcg tctgaaggag aagtaccgca
    11401 ccgtgggcga ggtgcgcggc cgcgggctgc tgctcggcat cgagctggtc gaggaccggc
    11461 agagccggaa gccgctcgac aagagcatca cccgggacct ctacctggag tgcctgcggc
    11521 gcggcctcgt ggcgatgacc tatgccccga ccatccggct caacccaccc ctcgtcatca
    11581 ccgaggagac ggcacgggcc gggctggcga tcctggacga agcgctcgga gtgacgctga
    11641 agcgccatgg gcgagactag gaggctccgg ggcgccgtca tcggcctcgg caacgtggcg
    11701 gtgcacggcc atctgcccgg ctggcggcaa cggcccgatg cggtcatcgt ggccgccacc
    11761 gatctccacc cggcgcggca ggccgagtgc cgggccgccc tgcccggggc gcgctggcac
    11821 gactcggtcg acgcgctcct ggccgccgac gacctcgact tcgtggacgt gtgcacgccc
    11881 ccgtcgagcc acgcggcgct catcgaggcc gcgctcgccc atgggctgca cgtgctctgc
    11941 gagaagcccc tggtggcctc gctcgccgag ctcgagcgcc tggtcgggct cgcccgtacc
    12001 gcgcgcgccg tcctccacac cgtgcacaac tggcaccagg cgcccctcgt gcgcgcgacc
    12061 gacgcgctca tccgcgaggg gcgcatcggg gccgtgcgcg aggtcgtctg gcggacgctg
    12121 cgcacccagc cggccgccgc ggcgggcggg gatagctggc gcgtggatcc cgcgatcgcg
    12181 ggcggcggtg tgctgaccga tcacggctgg cacgtctcgt acgtcgtccc gcgctgggtg
    12241 ggcgccccgc cggatgcggt gagcgcgcgg ctcgagaccc ggcggcacca tcgcttcgcg
    12301 gtcgaggata ccgcgacggt ccggctgcag ttccccgacg cggtggccga catcgtgctg
    12361 acgtgggccg ccgagacgcg cgagaaccag gctcacgtga tcgggaccgc ggggcgcatc
    12421 gacatcgagg acgatacggt ggttctccgc gtggacggcc gcgaggagcg tcaccggcgc
    12481 ccacccgccc tctctgacgg ctcccagcat ccggactggt tcgggccggt caccgacgag
    12541 ttcatcgccc gtgtgctcgg ccaggacccg agcagccgca atctggacga ggcggcggtg
    12601 tgcatcgcgg tggagggcgg cgcccgcgag tcgagccgcc gcggcggcgt gatgttcccc
    12661 ctggccgcgc gagtcgggtc ggccgggtga ccacgctctt ctccacggcc gtctccgtgc
    12721 ggagcctcct cgtgatcctg ccggccccgc ccggagccgg ctcgccgccg cccgggaccg
    12781 tgatcgcggg cctccccctc gtgcgccgca tcgtcctggc cgcggagcgc gccggcttcg
    12841 agcggatcct ggtccatccg cgggcctgcc cggagccgcg tctgctcgcg ggcaccgcgg
    12901 ccgccctgct cgacgccgac acgccggtgg cgcggcgggc ccggctcgtc ctggtgcccg
    12961 tgaacgtcct gccccaggcg cggtggctgc gcgccctgcg ggagatgccg ctcgatccgg
    13021 agacgctcgc ggtcgactcg tcgttgaccg cggtggtgga gagcgccggg atcgacgagg
    13081 tgctgcgctc ggccggccag gccgccgggg cggaggatct gatccccgcg ctctcgcgac
    13141 gcctcgcgcg cgccaccgag gcgagcgatc cggcgggccg cctcacgctc ggggacgcgt
    13201 cgggacccca ggccgcagag gactggctgc tgcggagcct catcaaggac aacgaaggat
    13261 tcatgtcccg ccatgtcgag cggcgcatct cgctcgcgat cacccggcgg ctggtgaaca
    13321 cgcgcatcac gccgaacgag atgacgctcg tgagcctcac catcggcctg ctcgccgcgc
    13381 cattcttcct gtccgcagag cccctgcagc agatcgtcgg cagcctcctc ttcctgactc
    13441 actccatcct cgacgggtgc gacggtgagc tggctcggct gaagttccag gaatccccca
    13501 tcggcgcgcg gctcgacttc tggggggaca acctggtcca cgtggccgtc ttcggctgca
    13561 tggccggggg ctgggcccgg gccgaggcct ccctctggcc gctgctcctg ggagccctgg
    13621 cgatcaccgg caccgccgcg tccgcctatc tcgtcgccca ccgcgacggg ggggcggccg
    13681 ccacggggcc tcccggccgc ctggccgacg cggtggccaa ccgcgatttc atctatctcg
    13741 tgctcgtgct cgcctgcttc ggccgggccc actggttcct gatcccggtc gcactcggca
    13801 caccaatctt catgatgctg gtccggtggg gcgcgggtcg acgccggccg acctgacgtg
    13861 ttttccgtcc tcgacgtcct gctgcgcggc ttcatcctcg ccgcgcaggc caccgcggtg
    13921 ggcggggtgt gctttctgct gctcgtggcc cgcgggccgg aggcgccgcg ctcgctgggc
    13981 ctgatcgctg ccgccgcggc cacgctcgcc cttggccagg ccctgtcgct cgccaacgag
    14041 ctgttcgtgc ttctccacga ggcggtctgg ccgctgcgcg cggcgctcga cacgctctac
    14101 gtgcaggtga gcgtcgtccg gatcgcggcc gggctcgcgc tggccgcgtg cgccttcggg
    14161 ctgcgccgcg cgggcgggcc gtcgcggctc tggccggcgg tggtggcgct ggccgccgtg
    14221 ctggtcgtgg cggctccgtg gacgagccac gcggccgcgc gactcgactc ccgcatcctg
    14281 ctgctgctgc tcgacggcgc ccatcagctc ggcgccgcgg tgtggatcgg cgggctcgtg
    14341 catctgaccg cggccgcgtt ccggcgcggc gatcggccct ggccggtggc tctgctccgg
    14401 cggttctcga ccctggcgct gagcgcggtc gccgtcctgg tgggcgcggg cgtgggcctc
    14461 acctggaact acgccccgga ccagaccgcg atcttcggca ccgcgtacgg tctcatggtg
    14521 ctgaccaagc tggcgatcct cggcggcctg ctcgtgctgg gcgcgctgaa cacccgggcg
    14581 atccgtcgga tggacggcgc tcgcgaggcc gatcagctca cgctccgccg cttcgtggag
    14641 gtggaggtcg gcctgggcct caccgtcctc ttcgcggccg cctcgctcac ctcgctgccg
    14701 cccgcggtgg acctgaccca cgatcgggcc acgctggcgg aggtcgcgac gcgcctcacc
    14761 ccgcggatgc cctcgctccg gtcgccctcg atctccgaga tgccgatcga cgacccgctg
    14821 gctccccgca acgacgccga tcgcgcctgg tccgagttca atcaccacgt gtccgggatc
    14881 ctggtcctca tcatgggcct gctggccctg ctgcacgcca ccggccgggc ccgctgggcg
    14941 cggcactggc cgctcgtctt tctgggcctg gccgccttca tgatcgtgcg caacgatccg
    15001 ggctcgtggc cgctcggccc cgagggcttc tgggagggct ggctcgtcgc gacggtggcg
    15061 cagcaccggg tcttcgtgct gctcgtggtc gtcttcggca tcttcgagtg gatggtccgc
    15121 agcggccggc tggcgtcgcg gcgggcggcc ctggtcttcc cgctactctg cgtggtcggc
    15181 ggcggcctgc tcctgagtca ctcgcacgcc gcgcagaacc tcaaggacga gttcctgctc
    15241 gaggtgagcc acgcgccgct cggcctgctg gccttgctga tcggctgggg gcgctggctc
    15301 gagcttcggc tccccgaggg gcggggg
//
LOCUS       DASUFA010000344        14485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1217322, whole genome shotgun sequence.
ACCESSION   DASUFA010000344 DASUFA010000000
VERSION     DASUFA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14485
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217322"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFX87_12625"
     CDS             <1..436
                     /locus_tag="VFX87_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995817.1"
                     /translation="MDGFAVCRTLRERDATRLVPVVVMTALQGVENRVKLLQHGADDV
                     LTKPVDERELIARVQAVLARKRAVDQRLGEITRVRDHFAKFVPDAVKRLVAANPDAPS
                     AASSTASPTRAVTSTRRRVTASWPSSKTATPPLTACGRSTRG"
     gene            373..780
                     /locus_tag="VFX87_12630"
     CDS             373..780
                     /locus_tag="VFX87_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4995818.1"
                     /translation="MAIFQDGDPTAHGVRAVNAGLMLLALTEALNQTELDNPLAIHIG
                     INSGLALVGSTRFEGRRGTRWTFTASGMVTTLAARLAGAAGDGEILAGPETVRRLGGR
                     YRLERVGHERLKNITEAIEVHRIISPPSKPVLR"
     gene            complement(794..1438)
                     /locus_tag="VFX87_12635"
     CDS             complement(794..1438)
                     /locus_tag="VFX87_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX4995819.1"
                     /translation="MTGARRHLPLVVASAALALLAGCARAVMTPPSVPAVAGMEEVGM
                     ASWYGAPHHGRRTSSGEVFDMHQLTAAHRTLPFGTRLLVTNRDTSQSAEVRVNDRGPF
                     VAGRILDVSYAAARLLGAVGPGIIPVRVRVLSLPPRSSNGDGPYTIQIGSFTTRARAE
                     ALRDTMPGATIAETTVAGETVYRVRVGAYPDRTRAGAAARDLTTRGYQALVVEQ"
     gene            1583..1780
                     /locus_tag="VFX87_12640"
     CDS             1583..1780
                     /locus_tag="VFX87_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995820.1"
                     /translation="MRLKRQDVERMMDERPAGSSLEEALEVFEVFASGSLADEVYVLD
                     DVGGKRIAIAPAALKAKYRKE"
     gene            1787..2614
                     /locus_tag="VFX87_12645"
     CDS             1787..2614
                     /locus_tag="VFX87_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX4995821.1"
                     /translation="MAIITISHEIGAGGPEIGQQLADRLRYRYVDQDLVSNAAQKYGL
                     LEEKLSHLDESKPSLFERFDAETRRYITVIQTALYDFAEQDNVVLMGRGGQWLLRGIP
                     HVLRVRIMAPFDVRVKRLTKKLAGPMGEQTNPRTVSELVRRDDAEKAGRMRYLYEVGI
                     TDPALYDLVVNTEKLMTAAVVGLLAGVVGQPELAATPASSQLVADRSLASRVQVALAT
                     HPETRKYRISVEARAGVVTLEGTAVMDEAMDVARGVEGVNEVKARQMELPPIPPFVA"
     gene            complement(2619..3815)
                     /locus_tag="VFX87_12650"
     CDS             complement(2619..3815)
                     /locus_tag="VFX87_12650"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX4995822.1"
                     /translation="MSRRPILIAVVALLLVAAGGGGYLLRDRLGRSVAAPAPSSPQAA
                     GSLALAEALQLGFVDVAQQVRPAVVHLGTIQRAKNRRGPNLPQGNDDPFFRDFFNQFF
                     GSEGPDSRSEFRRPGLGSGVIIDKRGLVLTNFHVVKGADEILIRLSDKREYRGQILGT
                     DPKTDLAVVKFQPDHELTVASLGNSDALRVGEWAIAIGNPFGLDQTVTVGVISATGRS
                     DVGIATYENFIQTDASINPGNSGGPLVNLKGQVIGVNTAIVAAGQGIGFAIPINMVKR
                     VVDQLVDKGKVVRGWLGVALQPLSPDLAQSLGLPGTNGAVVGSTIAGSPAAQAGLQQG
                     DVIVAYDSTPVDDYRHVQRLVAETRVGKSVTLQIVRKKQKMDVAITIAEVPDETGRRP
                     GPPPKP"
     gene            3905..4789
                     /locus_tag="VFX87_12655"
     CDS             3905..4789
                     /locus_tag="VFX87_12655"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4995823.1"
                     /translation="MRYRHVWPGFLLLAALALLLVGLLRWGDHFLSPGPTGGINRLLQ
                     LWAASRPAEESIAPPAAAAPSPAMDAPANPPVVAAPPVIVPVTPEASGPAAQPAPAPS
                     GSTPVASTPVPPVPAPVVKAPAPERAEEPREAAAGPTTPPPVRYALALGTFPLSEDAE
                     RVEAKLNQAGFSTVRFRLQTPAKLFAVQVSSLKDPEEGQATVERLQKEGHPPSVLLVG
                     RTGVTVRVGPPTPLRTAVQLAEKLRATGYDARVATEPTRAGQVSLRHGNFASRAEADA
                     ASREVARLGVPNEVIQVR"
     gene            4856..5230
                     /locus_tag="VFX87_12660"
     CDS             4856..5230
                     /locus_tag="VFX87_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4995824.1"
                     /translation="MPAKKILVVDDEPQVRQLMEHFLTERGYEVRIAENGRLALLALD
                     TFMADVVLLDMHMPEMDGLETLKRLAVRSPSLPVIMITVNDDMETTARLLQLGAADYV
                     PKPFNLDYLEQAINIQLSSARE"
     gene            complement(5278..7395)
                     /locus_tag="VFX87_12665"
     CDS             complement(5278..7395)
                     /locus_tag="VFX87_12665"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX4995825.1"
                     /translation="MRSLAASWRVPLAGSLAGLAIVVLAAANPLSFFDIAELKALDAQ
                     FTLRGPREPQSPIVVVTIDEDSFDGLNLAWPWPRAVHAKFLDIVGRGGPVAIGMDILF
                     TEPSSRGPADDVALGAAVDRVRDRIVLAAALTTVGDASFVKQALNPPIPEIRGRAAFG
                     SADYDSDADAFVRRATLTRNFQDRDWPGFDLLLYRLGERAGLARGAPPAGSELLVNYR
                     GGPSTFPMIAYHRVVTGEIPSEVFAGKIVLVGATTPTLHDIFPTPFASQSGMSGVEIH
                     AHTLETLFQGIPLRRAPRFLVAVVAVLAGAAAAWTATALRPLPAFGVVAAGVGGYLAA
                     SHGAFLWGRYWMEVVPVPLALLITYAGTVAKNFTQEQREKRRLSRFFSPDVVAEIVRH
                     KDDAKLAANRRRMTVLFSDIRGFTSMSEKMQPEEVVTFLREYLTVMTEAVFKHGGTVD
                     KYIGDAIMALYNVPFEAPDHALRAVRTALEFQERLQPLAERFAAKHGGTLACGVGIHT
                     GDAVVGTIGSEQRLEYTAIGDTINLGSRLEGLTKEFSVPIIISEATYAEVRGQFGVRD
                     LGEVTVKGKLIPVKIYAVLGPLLRPQASGVDVGTGAGTFNSRSAPRRRMEGHVSISDG
                     ELTVVAEASDISRAGIAVHAVPRRLELGEVVSLQIALRDHPEVLTIERAEVMWARKDT
                     AGLRFVDLSPATRAVLDTLTTQT"
     gene            7457..7867
                     /locus_tag="VFX87_12670"
     CDS             7457..7867
                     /locus_tag="VFX87_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995826.1"
                     /translation="MRVRTILLASFWVAVFLWVGYNGMQAIYSYFQTNDLTEQAFTGA
                     WERQRQRNPSDLYSAEFLTDLRSSVLMGARRAGIQVDPASIKVTADGGLVRVALSWTY
                     RTEPRNTWGFDTGLPVPLWLGRSFDPQLGARRMF"
     gene            7890..9284
                     /locus_tag="VFX87_12675"
     CDS             7890..9284
                     /locus_tag="VFX87_12675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4995827.1"
                     /translation="MSEHIVIAEDDEDLAFVLREALRRQRYEPEVAPTAGALLDKLKA
                     TPYDLILLDVRLPDMDGLDAIPKCRELAPDTPIIVMTAHGTRETAMEAMTRGAYDFFT
                     KPLKMDEFQVVVARALDRRHLQKQIKVLRESQPGGVEGLIGQSEALKRVVEMAHRAAP
                     TDLTVLILGESGTGKEVLARGIHRLSSRKDGPFVPVNCAAIPEGLLESELFGHERGAF
                     TGAVRARPGRFELAKEGTLFLDEIGDMPLPMQAKILRVLQEREFERVGGTRTIAADVR
                     VIAATNRDLEAAVAQGTFRQDLFYRLQGVGMHLPALRERVDDLPLLATHLLERAAKRL
                     NRAPAILSPEALRCLWAYAWPGNVRELQHVLEGAMVMSDGVITPEHLPPAIQRAAQAR
                     PAAAEPGPVLTGSLDEALEEWERRMILDALSQTQGVQARAAKVLGISERSLWYRIKKL
                     GIQVRTPEDGPPAG"
     gene            9323..10186
                     /gene="galU"
                     /locus_tag="VFX87_12680"
     CDS             9323..10186
                     /gene="galU"
                     /locus_tag="VFX87_12680"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX4995828.1"
                     /translation="MKVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPTIQYVVEEA
                     VASGLQEIILVTGRNKRAIEDHFDAAFELEYYLQDRGKMEELAQIKTISELASVSYVR
                     QKEPLGLGHAILCARPLVGDEPFGVFLGDDIIVSRVPCMRQLLDVYERHDGPVLAVMR
                     MPREEIGRYGVIAARGLGANVYEVQDLVEKPDPRDAPSDLAIIGRYVLTPDVFPILQE
                     TAPDSRGEIQLTNGLRALRGRRPIYAVEFEGKRHDTGEKLGFLKATVEFALARPDLAD
                     AFRAYLKSLKL"
     gene            10318..11532
                     /locus_tag="VFX87_12685"
     CDS             10318..11532
                     /locus_tag="VFX87_12685"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX4995829.1"
                     /translation="MKSQPKRAFHMASEPEIKAGEVSDVYFERTIEILRAKGLHKRVK
                     AEIRLKSFPQTDWTFGVLAGIEEAAALLEGLPVDVWAMDEGTLFGPEDTVLLIEGVYP
                     EWAKYETALLGFLCQASGIATKAARCKRAAGERQVISFGARRMHPALAPMIERNAFVG
                     GCDGVAVTKSAELIDADPTGTIPHALVLLFGDTVEALRAFHEVVDPKVRRVALIDTLQ
                     DEKFEAIRVAKALGRDLYAVRLDTPSSRRGDFFRILEEVRWELDLRGFNHVKILASGG
                     IDEYEILKLNSLVDGYGVGTSIANAPVLSFALDIMEIEGLPMAKRGKRSGAKQVWRFP
                     GAVQNVVLPAAAPAPVGPDGQPAEALLKPLIAGGRLVRDLPPPRTLREYVMEQMARID
                     ITGRDHGLRGDY"
     gene            11537..12322
                     /gene="trpC"
                     /locus_tag="VFX87_12690"
     CDS             11537..12322
                     /gene="trpC"
                     /locus_tag="VFX87_12690"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX4995830.1"
                     /translation="MGVLDEIVAHKRSELAELKPRRPLPDLIAACRGLAPARDFEAAL
                     RPAAGERVRLIAEVKRGSPSQGLFRADLDPVAQAGIYAGAGAAAVSVLTDARYFHGSL
                     DDLVRVRGAVAVPVLRKDFIVDEYQLWEARAAGADAVLLIVAALEDVALRDLLHAAKG
                     AGLATLVEVHTAGELDRALRVGAPVIGVNNRDLQTLTTSLEPSLRLLPQIPPGPVTVS
                     ESGLASGADVARVVAAGAHAVLVGETLLRAADVAAKVRELSLR"
     gene            12319..12939
                     /locus_tag="VFX87_12695"
     CDS             12319..12939
                     /locus_tag="VFX87_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX4995831.1"
                     /translation="MRIKICGITNLDDARLCVAAGADALGFIFVEGTPRYVTPETAAR
                     IVAALPPFVTPVGVFWDHAPGHVKAVAEQCGLGALQFHGDETPEDLHEFRLPVIKTLK
                     IAGPADLDRMAGYRVAAFLLDSPARWSEGQARPVIPWDVAAEMARRHPVLLSAGLTPD
                     NVGEAVRCVRPYGVDVNSGVEARPGRKDEARVIRFVTEARAAAAAP"
     gene            12936..14174
                     /gene="trpB"
                     /locus_tag="VFX87_12700"
     CDS             12936..14174
                     /gene="trpB"
                     /locus_tag="VFX87_12700"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX4995832.1"
                     /translation="MSPARLRPASLPDANGHFGRFGGRFVPETLMQPLVDLERAYHAA
                     RADTRFRRRLRELLSTYAGRPTPLYFAERLTRHVGGARIYLKREDLCHTGAHKINNVL
                     GQALLAQRMGKSRIIAETGAGQHGVGSATAAALLGLECEVYMGSVDVERQALNVFRMK
                     LLGARVIPVESGSKTLKDAVNEALRDWVTNVRTTYYLLGSVMGPHPYPMLVRDFQRVI
                     GDEARAQSRRTIGRLPDLIVACVGGGSNAMGLFWPFIEDRRVRIVGVEPGGHGIATGK
                     HGASLGSGAVGVLHGSMSYVLQNDDGQIAEAHSISAGLDYPGVGPEHSYYKDLGRFEY
                     VSVTDQEALEGFKALTKLEGIMPALESSHAVAHAMRAAARMKKTQSVLIGLSGRGDKD
                     VHTVAQALDGAAANGDGGRR"
     gene            14171..>14485
                     /locus_tag="VFX87_12705"
     CDS             14171..>14485
                     /locus_tag="VFX87_12705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX4995833.1"
                     /translation="MKRPAAAPRPASRLAAAFAALRARRERALVVYVTAGDPSLAMTR
                     RLVGEAARRGADVIELGVPFSDPLADGPVIQRASQRALAAGVTLPRVLELVREMRGEV
                     SVP"
BASE COUNT         2099 a         5132 c         5179 g         2075 t
ORIGIN      
        1 catggacggc ttcgcggtct gccggaccct gcgggagcgc gacgcgaccc ggctcgtccc
       61 ggtggtcgtc atgaccgcgc tgcagggtgt ggagaatcgt gtgaagctcc tgcagcacgg
      121 cgccgacgac gtgctcacca agccggtgga cgagcgcgag ctgatcgccc gcgtgcaggc
      181 cgtgctggcc cgcaagcgtg cggtggatca gcgcctgggc gagatcacgc gcgtccgcga
      241 ccacttcgcc aagttcgtgc ccgacgcggt caagcgtctg gtcgcggcca atcccgacgc
      301 gccttcggca gcttcctcga ccgcatcacc gacgcgggcg gtgacatcaa cgagacgtcg
      361 ggtgacggct tcatggccat cttccaagac ggcgacccca ccgctcacgg cgtgcgggcg
      421 gtcaacgcgg ggctgatgct gctcgccctc accgaggcgc tcaaccagac ggagctcgac
      481 aatccgctcg cgatccacat cggcatcaac tcggggctgg ccctggtcgg ctccacgcgc
      541 ttcgagggcc gccgcggcac ccggtggacc ttcaccgcga gcggcatggt caccacgctg
      601 gccgcccggc tcgcgggcgc cgcgggcgac ggcgagatcc tggccggtcc ggaaacggta
      661 cgccgtctcg gcgggcgcta ccgcctcgag cgggtcgggc acgagcggtt gaagaacatc
      721 accgaggcca tcgaggtgca ccgcatcatc tcgccgccct cgaagcccgt gcttcgctga
      781 ccgcggaccg cctctactgc tcgacgacga gggcctggta gccccgggtg gtgaggtcgc
      841 gggcggcggc gccggcccgg gtgcgatccg ggtaggcgcc gacgcgcacg cggtagacgg
      901 tctcgcccgc cacggtggtc tcggcgatcg tggcgccggg catcgtgtcg cggagggcct
      961 cggcccgcgc acgcgtcgtg aaggagccga tctgaatcgt gtaggggccg tccccgttgg
     1021 acgagcgggg gggaagcgag aggacccgca cccggaccgg gatgatgccc ggacctaccg
     1081 cccccaggag gcgggcggcc gcgtaggaga cgtcgaggat gcggcccgcc acgaacgggc
     1141 cgcggtcgtt gacccgcacc tcggcggact ggctcgtgtc gcggttggtc accaggaggc
     1201 gcgtgccgaa gggcagtgtc cggtgcgcgg cggtgagctg gtgcatgtcg aatacctcgc
     1261 cgctggacgt ccggcggccg tgatgcggcg ccccgtacca cgaggccatc cccacctcct
     1321 ccatgcccgc gacggccggg acggacggag gggtcatgac cgcgcgggcg cagccggcca
     1381 gcaaggccag cgccgccgat gccaccacga gcgggagatg cctccgggcg cctgtcatgg
     1441 cagctccatc ctgccatcgg ggccgggggt gggccaaggg ggggcctgtc ccgaccgggc
     1501 gggggccttg tcttcagcga ggtttcctga tactcaagta gcccagcttc caatggtcag
     1561 aatcatccgg caggcctgaa tcgtgcggct caagcgccag gacgtggagc gcatgatgga
     1621 cgagcggccg gccggcagct cgctcgagga agcgctcgag gtgttcgagg tcttcgccag
     1681 cggtagcctc gcggacgagg tctacgtgct cgacgacgtc ggaggcaagc gcatcgccat
     1741 cgcccccgcc gcgttgaagg cgaagtaccg gaaggagtga cacgccatgg ccatcatcac
     1801 catctctcac gagatcggcg cgggcggacc ggagattggc cagcagctgg cggaccggct
     1861 gcgttaccgc tacgtggacc aggacctcgt ttccaacgcg gcccagaagt acgggctgct
     1921 cgaggagaag ctctcgcatc tcgacgagtc gaagccctcg ctcttcgagc gcttcgacgc
     1981 ggagacgcga cgctacatca cggtgatcca gaccgcgctc tacgacttcg ccgagcagga
     2041 caacgtggtg ctgatggggc gcggcgggca gtggctcctg cggggcatcc cccacgtgct
     2101 gcgcgtgcgg atcatggctc cgttcgacgt gcgagtcaag cggctcacca agaagctggc
     2161 cgggccgatg ggcgagcaga ccaacccgcg caccgtctcg gagctcgtgc gtcgcgacga
     2221 cgccgagaag gccgggcgca tgcggtacct ctacgaggtc ggcatcaccg atccggcgct
     2281 ctatgatctc gtcgtcaaca ccgagaagct catgacggcg gcggtggtag gcctgctcgc
     2341 gggcgtcgtg gggcagcccg agctggccgc gacgccggcc agctctcagc tggtggccga
     2401 ccgctcgctc gcctcccggg tgcaggtcgc gctggccacc catccggaga cgcgcaagta
     2461 ccgcatctcg gtcgaggcgc gcgccggcgt cgtgaccctc gagggcaccg cggtcatgga
     2521 cgaggcgatg gacgtggccc gcggggtcga aggcgtcaac gaggtcaagg cgcgacagat
     2581 ggagctgcca ccgatcccgc cgttcgtcgc gtagctcgtc agggcttggg gggagggccg
     2641 ggacgccggc cggtctcgtc cgggacctcg gcgatggtaa tcgcgacgtc catcttctgt
     2701 ttcttccgca cgatctgcag cgtcacgctc ttgcccaccc gcgtctccgc caccagccgc
     2761 tgcacatgcc ggtagtcgtc cacgggcgtg ctgtcgtagg cgacgatgac gtcaccctgc
     2821 tggaggccgg cctgtgccgc cgggctcccg gcgatcgtcg agcccaccac cgcgccgttg
     2881 gtgccgggca gtcccaggct ctgggcgagg tcgggagaga gcggctgcag cgccaccccg
     2941 agccagccgc gtaccacctt gcccttgtcc acgagctggt ccaccacgcg cttcaccatg
     3001 ttgatgggga tggcgaagcc gatgccctgg cccgcggcca cgatggcggt attgaccccg
     3061 atgacctggc ccttgaggtt gacgagggga cccccggagt tgccgggatt gatggaggcg
     3121 tcggtctgga tgaagttctc gtaggtggcg atgcccacgt ccgagcgccc ggtggcgctg
     3181 atgacgccca ccgtcaccgt ctggtccagg ccgaagggat tgccgatggc gatggcccac
     3241 tcgccgaccc ggagcgcgtc cgaattgccc aggctggcca ccgtgagctc gtgatccggc
     3301 tggaacttga ccaccgcgag gtccgttttc gggtcggttc cgaggatctg tccgcggtat
     3361 tctcgtttgt cggagaggcg gatcaggatc tcgtcggcgc ccttgacgac gtggaagttg
     3421 gtgaggacca ggccccgctt gtcgatgatg acgccggatc ccaggccggg ccggcggaat
     3481 tcggagcggg aatcgggccc ttcggagccg aagaactgat tgaagaaatc gcggaagaag
     3541 ggatcgtcgt tgccctgcgg caggttgggg ccccgccggt tcttcgcccg ctggatcgtc
     3601 cccaggtgca cgacggcggg gcgtacctgc tgcgccacgt ccacgaagcc gagctgcaat
     3661 gcctcggcga gcgccaggct gccggcagcc tgcgggctgc tcggcgccgg cgcggccacg
     3721 ctgcggccga ggcggtcgcg cagcaggtac ccgccgcccc ccgcggcgac caggaggagg
     3781 gcgaccacgg cgatcagtat cgggcggcgg ctcatgtatg tactctacca gggggcccct
     3841 gcgaagcgga gcctgggggc gaccggatga tagcgactgc cgtcttggac tgtcaaggaa
     3901 gcccgtgaga taccgtcacg tctggccggg gtttctgctg ctcgccgcgc tggcgctgct
     3961 gctggtgggc ctgctgcggt ggggggatca cttcctgtcg cccgggccca cggggggcat
     4021 caaccggctg ctgcagctct gggcggcctc gcgccccgcc gaggaatcca tcgcaccgcc
     4081 cgcggccgcc gcgccgtcac cggccatgga cgcgccggcc aatccaccgg tggtcgcagc
     4141 gcctcccgtg attgttccgg tgacgccgga ggcttcgggg cctgccgccc agccggcgcc
     4201 cgcgccgtcc gggtcgacgc cggtggcgtc cacgccggtc ccgcccgtcc ccgcgccggt
     4261 cgtcaaggcg ccggcaccgg agcgcgccga ggagccgcgc gaggcggccg ccgggccgac
     4321 cactccgccg ccggttcgct acgcgctggc gctcggcacc ttcccgctgt ccgaagacgc
     4381 cgagcgcgtg gaggccaagc tcaaccaggc cggcttctcc accgtgcgct tccgcctgca
     4441 gacgccggcc aagctcttcg ctgtccaggt gtcctccctg aaggatccgg aggagggcca
     4501 ggccaccgtc gagcggctgc agaaggaggg gcatccgccg agcgtgctcc tggtcgggcg
     4561 caccggagtg acggtgcgcg tgggcccgcc gacgccgctg cgaaccgcag tgcagctggc
     4621 cgagaagctg cgggccacgg gctatgacgc gcgcgtggcc accgagccca cccgcgcggg
     4681 tcaggtgagc ctccggcacg gcaacttcgc ctcgcgtgcc gaggcggacg cggccagccg
     4741 ggaggtcgcc cgcctgggcg tccccaacga ggtcatccag gtccgctgac ccgggcggcc
     4801 ccttggccgc ctacggatct tggagataaa tccgccccga gagtagtatc gggtcatgcc
     4861 cgcgaagaag atcctcgtgg tggacgacga gccgcaagtg cgccagctaa tggagcattt
     4921 tctgaccgag cgcggttacg aggtgcgcat cgccgagaac ggccggctgg ccctgctggc
     4981 gctcgacacg ttcatggccg acgtcgtgct gctggatatg cacatgccgg agatggacgg
     5041 tctggagacc ctcaagcgcc tggccgtccg ctcgccctcg ctgccggtga tcatgatcac
     5101 cgtcaacgac gacatggaga cgacggcgcg cctgctccag ctcggcgccg ccgactacgt
     5161 ccccaagccg ttcaacctcg actacctcga gcaggccatc aacatccagc tctcctccgc
     5221 ccgcgagtag cggtctccgc cgcggtccgg ggtggcgggg ctccccggga ccgccggcta
     5281 ggtctgggtg gtcaacgtgt cgaggacggc gcgggtggcg ggcgagagat cgacgaagcg
     5341 gaggccggcg gtgtccttgc gcgcccacat cacctccgcg cgctcgatcg tcaacacctc
     5401 cggatggtcg cgcagggcga tctgcaggga cacaacctcg ccaagctcga ggcggcgggg
     5461 caccgcatgg accgcgatgc cggcgcggga gatatcgctt gcctcggcca ccacggtcag
     5521 ctctccgtcg gagatggaga cgtgcccctc catcctgcgg cgcggggccg agcgggagtt
     5581 gaacgtgccc gcccccgtcc cgacgtcgac cccgctcgcc tgcggccgca gcagggggcc
     5641 gagcaccgcg tagatcttca ccgggatcag cttgcccttc acggtgacct ctccgagatc
     5701 gcgcaccccg aactgcccgc gcacctccgc gtaggtggcc tcgctgatga tgatgggcac
     5761 gctgaactcc ttggtcagcc cctcgagccg cgagcccagg ttgatggtgt cgccgatcgc
     5821 ggtgtactcg aggcgctgct cggagccgat ggtgcccacc accgcgtcgc cggtgtggat
     5881 gcccaccccg caggccaggg tgccgccgtg cttcgcggcg aaacgctcgg ccagcggctg
     5941 caggcgctcc tggaactcga gcgcggtccg caccgcgcgc agcgcgtggt ccggcgcctc
     6001 gaagggcacg ttgtagagcg ccatgatggc gtcgccgatg tacttgtcca cggtgccccc
     6061 gtgcttgaac accgcctcgg tcatcacggt gaggtactcg cgcaggaacg tgacgacctc
     6121 ttccggctgc atcttctccg acatcgaggt gaacccgcgg atgtcggaga agagcacggt
     6181 catgcggcgc cggttggccg cgagcttggc gtcgtccttg tgccgcacga tctcggccac
     6241 cacgtcgggc gagaagaagc gtgagagccg tcgcttctcg cgctgctcct gcgtgaagtt
     6301 cttggccacc gtgcccgcat aggtgatgag gagggccagc ggtaccggca ccacttccat
     6361 ccagtagcgg ccccacagga aggcgccgtg gctggccgcc aggtagccgc cgacacccgc
     6421 cgcgacgacg ccgaaggcgg gcagcggccg gagcgcggtg gcggtccagg ccgccgccgc
     6481 gcccgcaagc accgccacca ccgccacgag gaagcgtgga gcccggcgca gcgggatgcc
     6541 ctggaacagg gtctccagcg tgtgggcgtg gatctcgacc cccgacatgc ccgactgcga
     6601 ggcgaacggg gtggggaaga tgtcgtgcag ggtcggggtg gtggcgccca ccagcacgat
     6661 cttgcccgcg aagacctccg acgggatctc cccggtcacc acgcggtggt aggcgatcat
     6721 cgggaacgtg ctgggccccc cccggtagtt gacgagcagc tcgctgcccg cgggaggcgc
     6781 gccgcgcgcc agtcccgcgc gctccccgag gcggtagagc agcaggtcga agcccggcca
     6841 atcccggtcc tggaagttac gcgtgagggt cgcgcggcgg acgaaggcgt cggcgtcgga
     6901 gtcgtagtcg gccgagccga aggcggcccg gccccggatc tcggggatgg gcgggttcag
     6961 cgcctgcttg acgaaggagg cgtcgcccac cgtggtcagg gccgccgcga ggacgatccg
     7021 atcgcgcacc cgatccaccg cggcgcccag cgcgacatcg tcggctgggc cgcgggacga
     7081 cggctcggtg aacaggatat ccatgccgat cgccaccggc ccgccccggc ccacgatgtc
     7141 gaggaacttg gcgtgaaccg cccgcggcca cggccaggcc agattgagcc catcgaacga
     7201 gtcctcgtcg atggtgacca ccacgatggg tgattgcggc tcgcggggcc cgcgcagggt
     7261 gaactgcgcg tcgagggcct tgagctcggc gatgtcgaag aagctcagcg ggttggcggc
     7321 ggccagcacc acgatggcca ggccggccag gctccctgcg agggggacac gccaggaggc
     7381 ggcgagggat cgcaccgaac ccagcctagc acaagggaag ttgccgccgc cgtggggaaa
     7441 cgtctacact tgatccatgc gggtccgcac gatcctcctc gcctcgttct gggtggccgt
     7501 gttcctgtgg gtcggttaca acggcatgca ggccatctac tcctacttcc agaccaacga
     7561 cctgaccgag caggcgttca cgggggcctg ggagcggcag cgccagcgca acccctccga
     7621 cctgtactcg gccgagttct tgaccgacct gcgcagcagc gtgctgatgg gggcccgccg
     7681 ggcgggcatc caggtcgatc cggccagcat caaggtgacc gcggacggcg ggctggtgcg
     7741 agtcgcgctg agctggacct accggaccga gccgcggaac acctggggct tcgacaccgg
     7801 cctgccggtg cctctctggc tcgggcgcag cttcgacccg cagctcggcg cccggcggat
     7861 gttctgatcc tccccgggcc cctgcggtca tgagcgagca catcgtcatc gcggaggacg
     7921 acgaggatct cgcattcgtc ctccgcgagg ccctgcgccg ccagcggtac gagcccgagg
     7981 tggcgcccac cgcgggtgcc ctgctcgaca agctcaaggc gaccccctac gacctcattc
     8041 tcctggatgt ccggctgccc gacatggacg gcctcgacgc gattccgaag tgccgcgagc
     8101 tcgcgccgga cacgccgatc atcgtcatga ccgcgcacgg cacgcgcgag accgcgatgg
     8161 aagcgatgac gcgcggggcc tacgacttct tcaccaagcc gctcaagatg gacgagttcc
     8221 aggtcgtcgt cgcgcgcgcg ctcgaccggc ggcacctgca gaagcagatc aaggtcctgc
     8281 gcgagagcca gccgggcggc gtcgaggggc tgatcgggca gagcgaggcg ctcaagcgcg
     8341 tggtggagat ggcccaccgc gccgctccca ccgacctgac cgtcctgatc ctgggcgaga
     8401 gcggcaccgg caaggaagtg ctggcgcggg ggatccaccg cctcagctcc cggaaggacg
     8461 gcccgttcgt gccggtcaac tgcgcggcga tccccgaagg cctgctcgag tccgagctgt
     8521 tcggccacga gcgcggggcc ttcaccggcg cggtgcgggc gcggcccggc cgcttcgagc
     8581 tcgcgaagga gggcacgctg ttcctggacg agatcggcga catgccgctg cccatgcagg
     8641 ccaagatcct gcgcgtgctc caggagcgcg agttcgagcg agtgggcggc acccgaacca
     8701 tcgcggccga cgtgcgggtg atcgcggcca cgaaccggga cctcgaggcg gcggtggcgc
     8761 aggggacgtt ccgccaggac ctgttctacc gtctccaggg cgtgggcatg cacctgcccg
     8821 cgctgcgcga gcgcgtggac gatctgccgc tcctggccac ccacctgctg gagcgcgcgg
     8881 ccaagcggct caaccgcgcg ccggccatcc tgtcgcccga ggccctgcgc tgcctgtggg
     8941 cctacgcgtg gccgggcaac gtgcgcgagc tgcagcacgt gctcgagggc gcgatggtga
     9001 tgagcgacgg cgtgatcacc cccgagcatc tccctcctgc gatccagcgc gcggcccagg
     9061 cccggcccgc cgccgcggag cccgggccgg tactgaccgg ctcgctcgac gaggcgctcg
     9121 aggagtggga gcgccgcatg atcctggacg cgctctcgca gacccagggc gtgcaggcgc
     9181 gcgcggccaa ggtgctcggc atctcggagc gcagcctctg gtaccggatc aagaagctgg
     9241 gcatccaggt gcgcacgccg gaggacggtc cgcccgcggg ctgagttccc tttcccgtgc
     9301 gcctcgtgta tcgtcgaact cgatgaaggt caggaaggcc gtattccccg ccgccggtct
     9361 cggtacccgc ttcctgcccg ccaccaaggc gcagccgaag gagatgctcc cgctcgtgga
     9421 caagcccacc atccagtacg tggtggagga ggcggtggcg tccgggctcc aggagatcat
     9481 cctcgtcacg ggacggaaca agcgcgcgat cgaggatcac ttcgacgcgg ccttcgagct
     9541 cgagtactac ctgcaggacc gcgggaagat ggaggagctc gcccagatca agacgatctc
     9601 cgagctggcg tcggtctcct acgtgcgcca gaaggagccg ctcggcctcg gtcacgccat
     9661 cctctgcgcg cggccgctcg tgggcgacga gcctttcggc gtgttcctgg gcgacgacat
     9721 catcgtgtcg cgcgtaccgt gcatgcgcca gcttctcgac gtgtacgagc gccacgacgg
     9781 cccggtgctg gcggtgatgc ggatgccgcg ggaagagatc ggccgctatg gcgtgatcgc
     9841 ggcgcgcggc ctcggggcga acgtctacga ggtgcaggat ctcgtggaga agccggaccc
     9901 gcgggacgcg ccctcggacc tggccatcat cgggcgctac gtgctcacgc ccgacgtgtt
     9961 cccgatcctg caggagaccg cgcccgactc ccgcggcgag atccagctca cgaacgggct
    10021 ccgcgcgctg cggggccggc ggcccatcta cgccgtcgag ttcgagggca agcgccacga
    10081 taccggcgag aagctcggct tcctcaaggc caccgtggag ttcgccctgg cccggccgga
    10141 cctggccgac gccttccgcg cgtatctcaa gagcctcaag ctgtaacacc tccggcgaaa
    10201 ggcgttgtgg ggcggcactt tgccacgcac gacggcctct cgattgattg acttccccgg
    10261 ggctttctgg gacaatccgt gcactttatt gcctggccgg gaggtgtcgg catctctatg
    10321 aagagccagc ccaagcgcgc cttccacatg gcctccgagc ccgagatcaa ggcgggcgag
    10381 gtctccgacg tctacttcga gcgcaccatc gagatcctgc gcgcgaaggg actccacaag
    10441 cgggtcaagg cggagatccg gctcaagagc ttcccgcaga ccgactggac cttcggcgtc
    10501 ctcgcgggca tcgaggaggc ggcggcgctg ctcgagggcc tgcccgtcga cgtgtgggcg
    10561 atggacgaag gcaccctctt cgggcccgag gacaccgtcc tcctcatcga gggcgtctac
    10621 cccgagtggg ccaagtacga gaccgcgctc ctgggcttcc tctgccaggc ctccggcatc
    10681 gccaccaagg ccgcgcgctg caagcgcgcc gcgggcgagc gccaggtgat ctccttcggg
    10741 gcgcggcgca tgcacccggc gctggccccg atgatcgagc gcaacgcctt cgtgggcggc
    10801 tgtgacgggg tcgcggtgac caagtccgcc gagctgatcg acgccgatcc gaccggcacc
    10861 attccccacg cgctcgtgct cctcttcggg gacaccgtgg aggcgctccg ggccttccac
    10921 gaggtcgtcg accccaaggt gcggcgcgtg gccctcatcg acacgctcca ggacgagaag
    10981 ttcgaggcga tccgggtcgc caaggctctc ggccgcgacc tctacgcggt gcgcctcgac
    11041 acgccgtcct cgcggcgcgg cgacttcttc aggatcctcg aggaggtgcg ctgggagctc
    11101 gacctgcgcg gcttcaacca cgtcaagatc ctcgcctccg gcggcatcga cgagtacgag
    11161 atcctcaagc tgaactcgct ggtggacggc tacggggtcg gcacctcgat cgccaacgct
    11221 ccggtgctct ccttcgcgct cgacatcatg gagatcgagg gcctaccgat ggccaagcgc
    11281 ggcaagcgct ccggggccaa gcaggtgtgg cgcttcccgg gcgcggtgca gaacgtggtg
    11341 ctccccgccg ccgcccccgc gccggtgggc cccgacggcc agcccgccga ggcgctcctc
    11401 aagccgctga tcgcgggcgg ccgcctcgtg cgcgacctgc ccccgccgcg cacgctgcgc
    11461 gaatacgtga tggagcagat ggcgcgcatc gacatcacgg ggcgggatca cgggctgcgc
    11521 ggcgactact agccgaatgg gcgtcctcga tgagatcgtc gcccacaagc gctcggagct
    11581 ggccgagctc aagccgcgcc gtcccctccc ggacctgatc gcggcctgcc gcgggctcgc
    11641 ccccgcgcgc gacttcgaag cggccctccg ccccgcggcg ggcgagcggg tacgcctgat
    11701 cgcggaggtc aagcggggtt cgccctccca ggggctcttc cgcgccgatc tcgatccggt
    11761 cgcccaggcc ggcatctatg cgggggccgg cgcggccgcg gtgtcggtgc tcaccgacgc
    11821 ccgctacttc cacggtagcc tcgacgacct cgtgcgagtg cgcggcgccg tggccgtccc
    11881 cgtgctgcgc aaagacttca tcgtggacga gtaccagctc tgggaggcgc gcgccgccgg
    11941 ggccgacgcg gtgctgctca tcgtggccgc gctcgaggac gtggccctgc gcgacctgct
    12001 ccatgccgcc aagggcgccg ggctcgccac cctcgtggag gtgcacaccg cgggcgagct
    12061 cgaccgcgcc ctgcgggtcg gcgcgccggt gatcggcgtc aacaaccgcg acctgcagac
    12121 gctcaccacg agcctcgagc cctcgctccg cctgctgccc cagatcccgc ccggcccggt
    12181 gacggtgagc gagagcgggc tcgcgagcgg tgccgacgtg gcgcgggtgg tggccgcggg
    12241 ggcccacgcg gtgctggtgg gggagacgct cctgcgggcg gccgacgtgg ccgccaaggt
    12301 ccgcgagctc tcgctgcgat gaggatcaag atctgcggca tcaccaatct cgacgacgcg
    12361 cggctctgcg tggccgcggg cgccgacgcg ctcgggttca ttttcgtgga gggcaccccg
    12421 cgctacgtca cccccgagac ggcggcccgg atcgtggcgg ccctgccgcc cttcgtgacg
    12481 ccggtgggcg tcttctggga tcacgcgccg ggccacgtga aggcggtggc cgagcagtgc
    12541 gggctgggcg cgctccagtt ccacggcgac gagacccccg aggacctgca cgagttccgg
    12601 ctgcccgtga tcaagacgct caagatcgcg ggccccgccg atctcgaccg catggccggt
    12661 taccgcgtcg ccgcgttctt gctcgactcg cccgcgcgat ggagcgaagg ccaggcccgg
    12721 ccggtgatcc cctgggacgt ggccgcggag atggcgcgcc ggcatccggt gctcctctcg
    12781 gccgggctca ccccggacaa cgtgggcgag gcggtgcgct gcgtccgccc ctacggcgtg
    12841 gacgtcaact ccggcgtgga ggcccgcccg gggcgcaagg acgaggcgcg ggtgattcgc
    12901 ttcgtgaccg aggcgcgcgc ggcggcggcc gccccatgag cccggcgcgc ctgcggcccg
    12961 cgtcgctccc ggacgccaac ggccacttcg gccgcttcgg cgggcgcttc gtccccgaga
    13021 cgctgatgca gccgctggtg gatctggagc gggcgtacca cgccgcccgc gcggacacgc
    13081 gcttccgccg acggctccgc gagctgctgt ccacctacgc gggccgcccg acgccgctct
    13141 acttcgcgga gcggctgacc cgtcacgtgg gcggggcgcg catctatctc aagcgcgagg
    13201 acctctgcca cactggcgcc cacaagatca acaacgtgct cggccaggcc ctgctcgcgc
    13261 agcgaatggg caagtcgcgg atcatcgcgg agaccggcgc gggccagcac ggcgtgggct
    13321 ccgccaccgc ggccgcgctg ctgggcctcg agtgcgaggt ctacatgggc agcgtggacg
    13381 tggagcgcca ggccctcaac gtcttccgca tgaagctgct gggggcgcgg gtgatcccgg
    13441 tggagagcgg ctcgaagacg ctcaaggacg cggtcaacga agcgctgcgc gactgggtca
    13501 ccaacgtgcg cacgacctac tacctgctcg gctcggtgat gggccctcat ccgtatccga
    13561 tgctcgtccg cgacttccag cgggtgatcg gcgacgaggc gcgcgcgcag tcccgccgga
    13621 cgatcggccg cctgcccgac ctgatcgtgg cctgcgtggg cggcggctcc aatgcgatgg
    13681 gcctcttctg gcccttcatc gaggaccggc gcgtgcgcat cgtcggggtc gagcccggcg
    13741 gtcacggcat cgcgaccgga aagcacggcg cctcgctcgg ctccggtgcg gtgggcgttc
    13801 tgcacgggag catgagctac gtgctccaga acgacgacgg ccagatcgcg gaggcgcact
    13861 cgatctcggc cggcctcgac taccccggcg tcggccccga gcactcgtac tacaaggacc
    13921 tgggccggtt cgagtacgtg tcggtgaccg accaggaggc gctcgagggc ttcaaggccc
    13981 tcacgaagct cgagggcatc atgcccgcgc tcgagtcctc ccacgcggtg gcccacgcga
    14041 tgcgggcggc cgcgcggatg aagaagaccc agagcgtgct gatcggcctc tccgggcgcg
    14101 gcgacaagga tgtgcacacg gtcgcccagg cgctcgacgg cgcggccgcc aacggcgacg
    14161 gcggccgccg atgaagcgcc cggccgccgc cccgcgtccg gcctcgcggc tcgccgccgc
    14221 cttcgcggca ctccgggcgc gccgcgagcg tgcgctcgtc gtctacgtca cggcgggcga
    14281 tccctcgctc gcgatgaccc ggcggctggt gggggaggcg gcccgtcggg gcgccgacgt
    14341 catcgagctg ggcgtcccat tctcggatcc gctcgcggac gggccggtga tccagcgggc
    14401 cagccagcgc gcgctcgctg cgggggtgac cctgccgcgc gtgctcgagc tggtgcgcga
    14461 gatgcggggc gaggtgtcgg tgccg
//
LOCUS       DASUFA010000345         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_88201, whole genome shotgun sequence.
ACCESSION   DASUFA010000345 DASUFA010000000
VERSION     DASUFA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88201"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VFX87_12710"
     CDS             <1..1468
                     /locus_tag="VFX87_12710"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX4995834.1"
                     /translation="LADRYHIPYPTAALDDAEAELTVREHGGAPRLPLPREAWRFARR
                     GPDGRVSPDASHLHLPGGFEPGRIYELIYRSQDPTLVGLGLLAVRDVGAWLRFGTAAA
                     GNPSAGRLDRAYAFGVSQTGRFLRHLLYLGLNEDESGRQVYDAVFPHVAGGRRGEFNM
                     RFGQPSLNATQSVGSQFPFTDAEQSDPLTGQRDGLLSRLAASGKMPKIFTVNTSAEYW
                     RGDASLVHTDVEGRADVEPPDHVRVYLFAGTQHTPGALPPPAADPNTGDRGRAPFNTV
                     DYAPLLRAALVNLDRWVTDGTAPPPSAVPRLADGTAVAAESTAAAFARIPGVRFPDRV
                     SRPARLDWGSDWVRGIVSNLPPKAGPAYVSFVSAVDADGNEVAGIRPPELLAPLATFT
                     GWNLRHPSQGAPGDLMSMMGSTFPLSPTAAARERAGDPRASMAERYEDRAGYLERVRA
                     AAQAAVARRHLLAEDIPAIVVRAGAQWDLFQSGLGGTE"
     gene            1468..>2227
                     /locus_tag="VFX87_12715"
     CDS             1468..>2227
                     /locus_tag="VFX87_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4995835.1"
                     /translation="MARKPHTVGIVGLGFGRAHIPAFQANGCEVVAVCQRDEATAQKI
                     AARYGVPRVFARWEEMLDKARPEIVVIAAPPALHKAIALAAFERGAHVLCEKPLAMDA
                     AEARAMIETAARAKRVGMTAFNWRFSASMLRFHEMVAAGHVGRPFHLGGRWLGARWAH
                     ESAASTWRMDRAQAGHGAMGDMGVHLIDLVRWSFGEFRRVSALGAVAHPSRTVPGGER
                     AADAEDYCAVLGELASGATVTLQASRAARGANENS"
BASE COUNT          270 a          815 c          825 g          317 t
ORIGIN      
        1 gctcgcggac cggtatcaca ttccgtatcc caccgccgcc ctcgatgatg cggaagccga
       61 gctgacggtg cgggagcacg ggggcgcgcc gcgtctgccc ctgccgcgcg aggcgtggcg
      121 cttcgcccgg cgcgggccgg acgggcgcgt gtcgccggac gcctcgcatc tgcacctccc
      181 cggcggtttc gagccgggcc ggatctacga gctcatctac cgctcccagg atccgacgct
      241 ggtgggcctc ggtctgctcg cggtgcggga cgtgggcgca tggttgcgct tcggcaccgc
      301 cgcagcgggc aatccgtccg ccggccggct cgaccgcgcc tatgccttcg gcgtctccca
      361 gacggggcgc ttcctccgcc atctgctcta tcttgggctc aacgaggacg agagcgggcg
      421 ccaggtctac gacgcggtgt tcccgcacgt ggcgggcggc cggcggggcg agttcaacat
      481 gcgcttcggc cagccttcgc tcaatgccac ccagagcgtg ggcagccagt tcccgttcac
      541 cgatgccgag cagtcggatc ccctgaccgg gcagcgcgac gggctcttgt cgcgcctggc
      601 cgcgagcggc aagatgccga agatcttcac cgtcaacacc tcggccgagt actggcgggg
      661 agacgcgtcc ctcgtgcaca ccgacgtcga gggccgcgcc gacgtggagc cgcccgacca
      721 tgtccgggtc tatctcttcg cgggcaccca gcacacgccg ggcgcgctgc ccccgccggc
      781 ggccgatccc aataccggtg accgtggccg cgcgccgttc aataccgtgg actatgcgcc
      841 gctgctgcgc gccgccctcg tcaacctcga ccgctgggtc accgacggga ccgcgccgcc
      901 gcccagcgcg gtgccgcggc tggccgatgg caccgcggtg gccgcggagt cgacggcggc
      961 cgccttcgcg cgcatcccgg gcgtgcgctt ccccgatcgc gtgagccggc ccgcgcggct
     1021 cgactggggc tcggactggg tgcgcggcat cgtctccaac ctgccgccca aggcgggtcc
     1081 ggcctatgtg agctttgtct cggcggtcga cgcggacggc aacgaggtgg cgggcatccg
     1141 gccgccggag ctgctggcgc cgctggccac cttcacgggc tggaatctcc ggcacccgtc
     1201 ccagggcgcg ccgggcgacc tcatgagcat gatgggctcg acctttccgc tttccccgac
     1261 cgcggccgcg cgggagcggg ccggcgatcc gcgcgcgtcc atggccgagc gctacgagga
     1321 ccgggccggc tatctcgagc gggtgcgcgc ggcggcgcag gcggcggtcg cgcggcgcca
     1381 cctcctcgcg gaggacattc ccgccatcgt ggtgcgcgcg ggagcgcagt gggacctctt
     1441 ccagtccgga ctcggaggaa cggagtgatg gcgcgcaagc cgcatacggt cggcatcgtc
     1501 gggctcggct tcggccgcgc ccatatcccc gcgttccagg ccaatggctg cgaggtggtg
     1561 gcggtgtgcc agcgggacga ggcgaccgcg cagaagatcg cggcccgcta cggggtgccc
     1621 cgggtcttcg cgcgctggga ggagatgctg gacaaggcgc gccccgagat cgtggtcatc
     1681 gcggcgccgc ccgcgctgca caaggccatc gcgctggccg cgttcgagcg gggcgcgcat
     1741 gtgctctgcg agaagccgct ggccatggac gcggcggagg cgcgagccat gatcgagacc
     1801 gccgcccgcg cgaaacgcgt ggggatgacg gcgttcaact ggcgattctc cgcttccatg
     1861 ctgcgctttc acgagatggt cgcggcgggc catgtgggcc ggccctttca cctgggcggc
     1921 cgctggctgg gggcccgctg ggcccacgag tcggcggcct cgacctggcg catggaccgc
     1981 gcccaggccg ggcacggcgc catgggggac atgggcgtgc acctgatcga cctcgtgcgg
     2041 tggagcttcg gcgaattccg gcgggtgtcg gcgctgggcg ccgtggccca tccctcgcgc
     2101 accgtcccgg gcggcgagcg agccgcggac gcggaggact attgcgccgt gctgggcgag
     2161 cttgcctcgg gcgccaccgt caccctccag gccagccgcg ccgcgcgcgg cgccaacgag
     2221 aattcgc
//
LOCUS       DASUFA010000346         6826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1217903, whole genome shotgun sequence.
ACCESSION   DASUFA010000346 DASUFA010000000
VERSION     DASUFA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217903"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1090)
                     /locus_tag="VFX87_12720"
     CDS             complement(83..1090)
                     /locus_tag="VFX87_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4995836.1"
                     /translation="MKFDIGILASQPLPVIVRQVQLAESLGFDTAWITDTHLVCRELW
                     VTLTACVAATSRIRLGPGITVPHTRHVSVTASAIATLHELAAGRLVLGIGTGGSAAET
                     MGLAVAKMARVATLEAMTTALRELLGGRSMRLDTGADARLAWLEHPAVIPLYLAGSGP
                     RMLDAAGRLGDGAIMYSGVAPWLLEAALGCVHAGARAAGRAPGAVDIAIWAPTSIASD
                     RALARDHVRGRVASALRHAFPVRWSPEDQAVIDQVRAAYDSYQHATAASKHRLLVTDR
                     FVDLMALAGTPDDVREQVLRVEAVGGVGRIILLPQVPGQDFVQREQVLRLFADQVMAR
                     L"
     gene            1150..2439
                     /locus_tag="VFX87_12725"
     CDS             1150..2439
                     /locus_tag="VFX87_12725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4995837.1"
                     /translation="MTLESGNGQRFDVAVIGGGPGGSSAATALARRGRRVLLLERDRF
                     PRFHIGESQLPWINGVLEELGVHDTIAKAGFVEKWGASFWEIDGTPASYVDFTAAVET
                     PIPRTFQVPRARWDELLLRHAEQSGVIVREGHRAVDAAFEPDGVTLRYAGPDGVEQTT
                     RVAAVVDASGRAGFLARKLGRYEHDPVLEQIAVHAQYEGVPRSEGRPAGDIRVLTRPD
                     VGWIWLIPISETVMSVGAVVPTELHRREAKATPEESLDHYLRTTPASPTVMRDARRVS
                     PARFDADYSYLATRMTGDRWIAVGDSGAFLDPIFSTGVLLAMQGGLEAAAAIDAGLRA
                     GDLSARRFARYERTVRTRYHYFRRFVVGFYDPYFRRLLFRRSRRLGIYEAVLSALSGN
                     WRPTLGTRLRIQLFFAIIAVKRLFRVAPPEDAGVWRR"
     gene            complement(2436..3041)
                     /locus_tag="VFX87_12730"
     CDS             complement(2436..3041)
                     /locus_tag="VFX87_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4995838.1"
                     /translation="MDARDLLLDEHARMHASSVTGEKGTLAERTFGGLTDEQMRARPR
                     EDLNSLAWLMWHIARAEDIFSNIILSGRDQVVDDGWLARLKISRRDFGIGMTKPEVAE
                     LTAQVDVAALRDYRDAVGRRTQKVIRGFGPADWQGELQASALEKAASLGCFGPKGEMV
                     AKAFTGRPRLGILGGLLVTHAAMHMGEAQTVRTAGGFGSGI"
     gene            complement(3059..3964)
                     /locus_tag="VFX87_12735"
     CDS             complement(3059..3964)
                     /locus_tag="VFX87_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX4995839.1"
                     /translation="MLSPADVKFYQDQGYLVVPDVLDAATLVAIRAELARILDGARAV
                     TTHTDRYDLEPGHRPDDPRVRRIKQPHRFYPVSQDLMRHPRLLAVLQALLGRDLRLHG
                     SKINLKSPRYGSPVEWHQDWAFYPHTNDDLLAVGVMLDDCTSDNGPLLVVPGSHRGPI
                     FNHHTGDHPAGGYFCGAIDPAAIRDEIARAVPLTGRAGSMSFHHVRLVHGSAQNVSSL
                     PRTLLLYEFGAADAWPLMGVADLAEFDGRLITGEPTIEPRMVPAPVRLPLPPARRQGS
                     IYENQSDAARRYFPVTDEPAPTPRT"
     gene            4087..5448
                     /locus_tag="VFX87_12740"
     CDS             4087..5448
                     /locus_tag="VFX87_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4995840.1"
                     /translation="MLDQRISRRSLLAGAAGTSALLTLPKSAYAQGPAVKKGTSLRLW
                     ILKTYVEPTNKAIEASAQRWAQKHGASVTVEYFTFEDMQTKYVAAIENKSTPDVGQLE
                     TGAPARFSGMGQLVDLTSFARQIEGQVGKPPANVAPVTMVGDKIYALPWYVMAGFWYV
                     WRDVFAKAKVKLPNTFEEAKQAAAALNRPKDNFYGMGQSWNRTSDGYGVMQSLMYSYG
                     AGWADKAGKYKSIKTAQMQAAMKWATDIYKDGIQPADTLTWTGSGNNENFIAKNIAQT
                     SNGPSITFALENALAKATDAKERKTREEAIANHVALPHPAGPEGRRMWAIAMSFGIFK
                     NSKDPEAAMSLIAHMLAPEETVSVMKDSYGQFAPVMSKARDASKDYFNKNDNYRTFGR
                     AAEWFVPTGFPGPVTAAAAEVQASNVLTDAPAKVIVDKWSVDQAIDWEDKKIKEIYDT
                     LRG"
     gene            5483..6388
                     /locus_tag="VFX87_12745"
     CDS             5483..6388
                     /locus_tag="VFX87_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX4995841.1"
                     /translation="MRPRASLWKRARIREAGTALLFLLPLLVLVVALIVYPVYRAVWL
                     SLTDKLVGYPERFVGLKNYFYLAEDDTFREVVRNSFVFTVASVGLKVMTGVLMALVLN
                     NVIRGRNFFRGLFLLPWITSTVIIALTWRWMFDAFPGRGFFNSVLLDLGLLSRPIAFM
                     ATPEGAMMAVIVANWWRGFPFFGVSFLAGLQAIPRELYEAASVDGATAWRRFWHITLP
                     GLKHVMIVTTLLSFILTINDFNIIYVMTRGGPGSATQIFATYSYQVAFNQLRWGRGVT
                     ICLFVVPILVLGIILISRYLLRDRR"
     gene            6391..>6826
                     /locus_tag="VFX87_12750"
     CDS             6391..>6826
                     /locus_tag="VFX87_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4995842.1"
                     /translation="MRLPLKALTWFGMGLFLVVILVPFWWIASMSFKTYEQIQFATSI
                     YLPAPFTWENYSGLWTETRFPTWLRNSLITALVVTAITTVVASLGGYAVARLRFPGRE
                     SVASLILILYLIPPALLFIPLYRVLAELGATNHLLSLFLSYPT"
BASE COUNT         1007 a         2344 c         2438 g         1037 t
ORIGIN      
        1 cggtcgtggt cagagccccc ctgccgcccc gcccgccgcc gcctgccgcg ccccgccggg
       61 atcgcgtgcc gcgagggttt cgctagaggc gggccatcac ctggtcggcg aagaggcgga
      121 ggacctgctc gcgttggacg aagtcctggc cggggacttg cgggagaagg atgatgcggc
      181 ccacgccgcc aacggcctcg acacgcagca cctgctcgcg cacgtcgtcg ggcgttccgg
      241 ccagggccat caggtctacg aagcggtcgg ttacgagcag gcggtgcttt gaggcggcgg
      301 tggcgtgctg gtaggaatcg taggcggcgc gaacctggtc gatgacggcc tggtcctcgg
      361 ggctccagcg caccgggaag gcgtggcgca gggcggaggc gacgcggccg cgcacgtggt
      421 cgcgggcgag cgcgcggtcg gatgcgatcg aggtgggggc ccagatggcg atgtccaccg
      481 cgccgggggc gcggccggcc gccctggctc cggcgtggac gcagccgagc gcggcttcca
      541 gcagccacgg ggcgacaccc gagtacatga tcgcgccgtc gcccagccgc ccggcggcgt
      601 cgagcatccg cgggcctgag ccggcgaggt agagcgggat cacggcgggg tgctcgagcc
      661 aggcgagccg cgcgtccgcg ccggtgtcga ggcgcatcga gcggccgccc aggagctccc
      721 ggagcgcggt cgtcatggcc tcgagcgtgg ccacccgcgc catcttcgcc accgccaggc
      781 ccatggtctc ggcggcgctg cccccggtgc cgatgccgag cacgagccgt cccgcggcca
      841 gctcgtggag cgtggcgatg gcgctcgcgg tcaccgagac gtggcgcgtg tgcggcaccg
      901 tgatgccggg cccgaggcgg atgcgcgagg tcgcggctac gcaggcggtg agcgtgaccc
      961 agagctcgcg gcagaccagg tgggtgtcgg tgatccacgc ggtgtcgaag ccgagcgact
     1021 ccgcgagctg gacctggcgg acgatgaccg gtaggggctg gctcgcgagg atgccgatgt
     1081 cgaacttcat gggcgcggcc gagaatcggc cgaaagcgtc ggcgcgtcaa ggggtatgct
     1141 aggggcccta tgaccctcga gagcgggaac ggacagcgat tcgacgtggc ggtgatcggc
     1201 ggtgggccgg gcggctccag cgccgccacc gcgctcgcgc gtcgcgggcg ccgcgtgctc
     1261 ctgctggagc gcgaccggtt ccctcgattc cacatcggcg agtcgcagct cccgtggatc
     1321 aacggggtgc tggaggagct gggcgtccac gacacgatcg ccaaggccgg gttcgtggag
     1381 aagtggggcg cgagcttctg ggagatcgac gggacgccgg cctcctacgt ggacttcacc
     1441 gcggcggtgg agacgccgat cccgcggacc ttccaggttc cgcgggcccg ctgggacgag
     1501 ctgctgctgc gccacgccga gcaatcgggc gtcatcgtgc gggaaggaca ccgggcggtg
     1561 gacgcggcct tcgagcccga cggcgtcacc ctgcgctacg cggggcccga cggcgtcgag
     1621 cagaccacgc gggtcgcggc ggtcgtggac gcctccggcc gcgccggctt cctggcccgc
     1681 aagctcggtc gctacgagca cgaccccgtg ctggagcaga tcgcggtgca cgcccagtac
     1741 gagggtgtcc cgcgctccga gggccgcccg gccggcgaca tccgcgtgct cacgcgaccc
     1801 gacgtcgggt ggatctggct gatcccgatc tcggagacgg tgatgagcgt gggcgcggtg
     1861 gtgccgacgg agctgcaccg gcgggaggcg aaggccacgc cggaggagag cctcgaccac
     1921 tatctgcgca ccacgccggc ctcgccgacc gtgatgcggg acgcccggcg cgtgtcgccg
     1981 gcgcgcttcg acgccgacta ctcgtacctg gccacgagga tgacgggtga tcggtggatc
     2041 gcggtgggcg attcgggcgc cttcctcgat ccgatcttct cgaccggtgt gctcctggcc
     2101 atgcagggcg ggctcgaggc ggcggcggca atcgatgcgg gcctgcgggc gggcgacctc
     2161 tcggctcgcc gcttcgcgcg ctacgagcgg acggtgcgca cgcgctacca ctacttccgc
     2221 cgcttcgtgg tgggcttcta cgacccgtac ttccgccggt tgctgttccg gcgctcgcgg
     2281 cggctcggga tctacgaggc ggtgctctcc gcgctgtccg ggaactggcg cccgaccctc
     2341 ggcacgcgcc tgcgcatcca gctcttcttc gcgatcatcg ccgtcaagcg cctgttccgg
     2401 gtggcgccgc ccgaagacgc cggagtctgg cgccgctaga ttccggagcc gaagccgccc
     2461 gccgtccgca ccgtctgggc ctcgcccatg tgcatggcgg cgtgggtgac gagcaggccg
     2521 cccaggatgc cgaggcgcgg ccggccggtg aaggccttcg cgaccatctc gcccttgggg
     2581 ccgaagcagc ccagcgaggc cgccttctcg agcgcgctgg cctgcagctc gccctgccag
     2641 tcggccggcc cgaacccgcg gatgaccttc tgggtccggc ggccgaccgc gtcccggtag
     2701 tcgcgcaggg cggcgacgtc cacctgggcc gtcagctccg cgacctccgg cttggtcatg
     2761 ccgatgccga agtcgcgccg cgagatcttg aggcgcgcga gccacccgtc gtccacgacc
     2821 tggtcccgcc cgctcaggat gatgttggag aagatgtcct cggcgcgcgc gatgtgccac
     2881 atcagccacg cgagcgagtt gaggtcctcg cgcggccgcg cccgcatctg ctcgtcggtg
     2941 aggccgccga aggtccgctc ggccagggtt cccttctcgc cggtgacgct gctggcgtgc
     3001 atgcgggcgt gttcgtcgag gagaagatcg cgcgcgtcca tggatttctc cgctgggatc
     3061 aggtccgggg agtgggggcc ggctcgtcgg tgaccgggaa gtagcgtcgc gccgcgtccg
     3121 actggttctc gtagatcgag ccctggcgcc gggcgggcgg caggggcagg cgcaccggcg
     3181 ccggcaccat ccggggctcg atggtcggct cgcccgtgat gaggcggccg tcgaactcgg
     3241 cgagatccgc gacgcccatc aggggccagg cgtcggcggc gccgaattcg tagagcagca
     3301 gggtgcgggg caggctcgag acgttctggg ccgagccgtg cacgagccgc acgtggtgaa
     3361 agctcatcga gcccgcgcgt ccggtgagcg gcaccgcccg cgcgatctcg tcgcggatcg
     3421 cggcggggtc gatggcgccg cagaagtagc cgcccgcggg gtggtcgccc gtgtggtggt
     3481 tgaagatcgg gccgcggtgg ctgcccggca ccaccaggag cgggccgttg tcgctggtgc
     3541 agtcgtcgag catcacgccc accgcgagca ggtcgtcgtt ggtgtgcggg tagaaggccc
     3601 agtcctggtg ccactccacc ggcgagccgt agcgcggcga cttgagattg atcttggagc
     3661 cgtgcagccg gaggtcccgg ccgaggaggg cctgcagcac cgccagcagg cgcgggtgcc
     3721 gcatgaggtc ctgggagacc gggtagaagc gatgcggctg cttgatgcgc cgcacgcgcg
     3781 gatcgtccgg gcgatggccc ggctccagat cgtagcgatc ggtgtgcgtg gtcaccgcgc
     3841 gcgcgccgtc gaggatgcgc gcgagctcgg cgcggatcgc gaccagcgtg gcggcgtcga
     3901 gcacgtcggg aaccacgaga tatccctgat cctggtagaa cttcacgtcg gcgggcgaga
     3961 gcatggagca aatctccttg cgcgaaatgt tagcacgaac cggcggcact ccgcccttga
     4021 caggggggcg cgggtgaccg acattcggag cgcgtccgga tcaaagacga tcaaggggga
     4081 cacgccatgc tcgatcagcg catcagccgc cgctcactcc tcgccggggc cgccgggact
     4141 tccgcgctgc tcacgctgcc caagtcggcg tacgcccagg gccccgcggt caagaagggc
     4201 acgagccttc gcctctggat cctcaagacc tacgtggagc ccaccaacaa ggccatcgag
     4261 gcaagcgccc agcgctgggc gcagaagcac ggggccagcg tgacggtgga gtacttcacc
     4321 ttcgaggaca tgcagacgaa gtacgtggcg gccatcgaga acaagagcac ccccgacgtg
     4381 ggccagctcg agacgggcgc gcccgcgcgc ttcagcggga tgggccagct cgtggacctc
     4441 acgtcgttcg cccgccagat cgaggggcag gtcggcaagc cgcccgccaa cgtggcgccg
     4501 gtgacgatgg tgggcgacaa gatctacgcg ctgccgtggt acgtgatggc cgggttctgg
     4561 tacgtgtggc gtgacgtctt cgcgaaggcc aaggtgaagc tgcccaacac cttcgaggag
     4621 gccaagcagg ccgccgcggc gctcaaccgg cccaaggaca acttctacgg catgggccag
     4681 agctggaacc gcacctcgga cggctacggg gtgatgcaga gcctcatgta ttcctacggc
     4741 gcgggctggg cggacaaggc cggcaagtac aagtcgatca agacggcgca gatgcaggcg
     4801 gccatgaagt gggccaccga catctacaag gacggcatcc agcccgccga cacgctgacc
     4861 tggaccggct cgggcaacaa cgagaacttc atcgccaaga acatcgcgca gacctcgaac
     4921 gggcccagca tcaccttcgc gctcgagaac gcgctggcca aggccaccga cgccaaggag
     4981 cgcaagaccc gcgaggaggc gatcgccaac cacgtggcgc tgccgcaccc ggcggggccc
     5041 gagggccggc gcatgtgggc catcgcgatg agcttcggca tcttcaagaa cagcaaggat
     5101 cccgaggccg cgatgagcct gatcgcgcac atgctcgccc ccgaggagac ggtgtcggtc
     5161 atgaaggatt cctacgggca gttcgcgccc gtgatgtcca aggcgcgcga cgcctccaag
     5221 gactacttca acaagaacga caactaccgc acgttcggcc gggccgcgga gtggttcgtg
     5281 cccaccggct tcccgggccc ggtgaccgcg gccgccgcgg aggtgcaggc gagcaacgtg
     5341 ctcaccgacg cccccgccaa ggtcatcgtg gacaagtggt cggtggatca ggccatcgac
     5401 tgggaggaca agaagatcaa ggaaatctac gacacccttc gcgggtgacg tggcccttcc
     5461 tgcagcatcc caccttcccg tgatgcggcc gcgcgcttcc ctctggaagc gcgcgcgcat
     5521 ccgggaggcg gggacggcgc tcctcttcct gctgccgctg ctggtcctgg tggtggccct
     5581 gatcgtctat ccggtctacc gggcggtctg gctctcgctc accgacaagc tggtgggcta
     5641 tcccgagcgg ttcgtcggcc tcaagaacta cttctacctc gccgaggacg acaccttccg
     5701 cgaggtcgtc cggaacagct tcgtgttcac ggtggcctcg gtggggctca aggtcatgac
     5761 cggcgtgctc atggccctgg tgctcaacaa cgtgatccgg ggccgcaact tcttccgcgg
     5821 gctcttcctg ctgccatgga tcacctcgac cgtgatcatc gcgctcacct ggcggtggat
     5881 gttcgacgcc ttcccgggcc ggggcttctt caacagcgtg ctgctcgacc tggggctgct
     5941 gagccgcccg atcgccttca tggccacccc ggagggggcg atgatggcgg tgatcgtggc
     6001 caactggtgg cgcgggttcc ccttcttcgg ggtctccttc ctggccgggc tgcaggcgat
     6061 cccgcgcgag ctctacgagg ccgcgtcggt ggacggggcc accgcctggc ggcgtttctg
     6121 gcacatcacg ctgcccggcc tcaagcacgt gatgatcgtc accacgctgc tgagcttcat
     6181 cctcaccatc aacgacttca acatcatcta cgtgatgacc cggggcggac cgggcagcgc
     6241 gacccagatc ttcgcgacct actcctacca ggtcgcgttc aaccagctcc gctggggtcg
     6301 cggcgtcacc atctgcctct tcgtggtgcc gattctcgtc ctcggcatca tcctgatcag
     6361 ccgctacctg ctccgggaca ggagataatc atgcggctcc ccctgaaagc cctcacctgg
     6421 ttcggcatgg gcctgttcct cgtcgtgatc ctggtcccgt tctggtggat cgccagcatg
     6481 tcgttcaaga cctacgagca gatccagttc gccaccagca tctacctgcc cgccccgttc
     6541 acctgggaga actactccgg gctctggacc gagacgcgct ttcccacctg gctccgcaac
     6601 agcctgatca ccgccctggt ggtgaccgcc atcaccaccg tggtcgcgag cctcggcggc
     6661 tacgcggtgg cgcggctgcg cttccccggc cgcgagtcgg tggcgagcct gatcctgatc
     6721 ctctacctga tcccgcccgc gctgctcttc atcccgctct accgggtgct cgccgagctg
     6781 ggggcgacca accacctcct gtcgctgttc ctgtcctacc cgacct
//
LOCUS       DASUFA010000347         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_520278, whole genome shotgun sequence.
ACCESSION   DASUFA010000347 DASUFA010000000
VERSION     DASUFA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520278"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFX87_12755"
     CDS             complement(<1..201)
                     /locus_tag="VFX87_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995843.1"
                     /translation="MDYHHLLEATGIVVSGVLFYSLAYGWFAPDDPRRRPFWIVTLGL
                     AWGALTVILMISRIETREGVFID"
     gene            complement(201..3008)
                     /gene="uvrA"
                     /locus_tag="VFX87_12760"
     CDS             complement(201..3008)
                     /gene="uvrA"
                     /locus_tag="VFX87_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX4995844.1"
                     /translation="MSQPVWLRIEGARQNNLKNVSVDIPHDRVTVITGVSGSGKSSLA
                     FDTLFAEGQWRYIESLSTYARMFLERLDRPDVDRIEHIRPAIALEQKNPVRTARSTVG
                     TATELHDYLRLLFAKIGRVHCPTCGAEARSDSADQVADALLRDHPGARALICFPLPVE
                     AARDPRALFATLLQRGFARVKAGDAVLDLAEVTGRPDGVPADLDPARLAVVLDRVVIG
                     PDSRRRITDSLETALAEGEGRASVDLLERGIVPVSRDFHCPSCGVALARPRPLLFSFN
                     HPLGACPECKGFGNVLRYDEARVVPDPTQSLAEGAVEPWSHPSGRWYQKQLLKAAKKR
                     GVDVGRPYAELSAEDRQWVYDGGGGLTGIRGFFEEVESYRYKLHVRVFLSRYRSQSPC
                     PRCEGRRLRPEALAVRVGGASIAELGERTVEDLDALFATLTLSPWEAGVARDVLKMLR
                     AKLSFLLRVGLGYLTLGRQTRTLSGGEAQRINLANQLGAQLTGTLYVLDEPSIGLHAR
                     DTARLAELCRELASGGNTVVIVEHDRALIEAADYVIEMGPGSGERGGAMVFAGTQAEF
                     RKDPRSLTARYLSGRDTIPLPLARREGRRSLVLEGARANNLKDVTVRIPLHTLTCVTG
                     VSGSGKSTLVHDTLYRAVARHFKVDFAVAGAHDALRGLQHLKGIRLIDQEPIGRTPRS
                     NPVTYVKAFDEIRRLFAGLPRAQALGLGPGAFSFNVPGGRCDTCEGDGFQKLEMYFVE
                     DVYVTCQECEGRRYRPDVLQVTYKSRDISQVLQQTVDEAVDFFASEPVLQRRLKVLQE
                     VGLGYLRLGQPATKLSGGEAQRLKIAAELSARPTTDHLFILDEPTTGLHLDDVRKLLL
                     VLNRLVDAGNTVLVVEHHLDVIKTADWVVDLGPEAGSAGGELVAEGTPEQVAQVGASY
                     TGKFLRELLPRPTGRAAAGRS"
     gene            3167..3625
                     /locus_tag="VFX87_12765"
     CDS             3167..3625
                     /locus_tag="VFX87_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX4995845.1"
                     /translation="MGEGADTALRARGLRLTGPRRVVLDVVRATESHPTAEWVHQMVR
                     RRLPRVSLGTVYRNLRLLVAEGLVKELPGPHARFDGNLSEHHHFTCLACGRISDVPAP
                     ANDPHSRALCGRVSAQSGGSVTHHRIEFYGRCPECQRRAGGRARSRRPTP"
     gene            3681..4130
                     /locus_tag="VFX87_12770"
     CDS             3681..4130
                     /locus_tag="VFX87_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX4995846.1"
                     /translation="MAGKPLKGSKSVDNLKEAFAGESQANRRYLYFARVADIEGFPDV
                     AGLFKDTADAETGHAFGHLDFLKEVGDPATGEPIGKTEKNLKAAVAGETYEYTQMYPG
                     MAKTARDEGYPELAEWFETLAKAEKSHAGRFTKGLNQVAGKEPADAI"
     gene            4224..>5246
                     /locus_tag="VFX87_12775"
     CDS             4224..>5246
                     /locus_tag="VFX87_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX4995847.1"
                     /translation="MTLDIRVPEFWQLDRVDTELRRVYDVCAGCRRCLPLCPSFKVMF
                     DRLDVDAVDGDVEKLPAADVKEVVDLCYQCKLCYNHCPYTPPHRWAVDFPRLMLRARS
                     AEARAKGVTLQDRFLGNTELVGRIGSLTAPISNWMNAVWVNRKFMEKAVGIHARRNLP
                     PFHRETFSRWFDGRKPRLRSAPARDRVAFFTTCTVEYNDPATGKAAVAVLEKNGVDVT
                     LPAQRCCGMPYLDGGAVTEAKRLIAANVKSLAEQVRLGREIVVPGPTCSYMLKQEYPW
                     LDGSDDANLVAAHTRDLFEYLSALHEKGRLDTSFPNAPGTIAYQLPCHLKAQNMGTRS
                     ADVLRLT"
BASE COUNT          813 a         1821 c         1899 g          713 t
ORIGIN      
        1 gtcgatgaag acgccctccc gggtctcgat gcgggagatc atcaggatca ccgtgagggc
       61 gccccaggcc aggcccagcg tgacgatcca gaagggccgg cggcgcggat cgtccggagc
      121 gaaccagccg taggccaggg agtagaagag gacgcccgag acgacgatgc ccgtcgcctc
      181 gagcaggtgg tgatagtcca tcagctccgg ccggccgccg cgcgcccggt gggccggggc
      241 agcagctcgc gcaggaattt cccggtgtag gacgcgccga cctgcgcgac ctgctcgggc
      301 gtgccctcgg ccaccagctc cccgccggcc gagccagcct cggggccgag gtccaccacc
      361 cagtccgcgg tcttgatcac gtcgaggtgg tgctcgacca cgagcacggt gttgcccgcg
      421 tccaccaggc ggttgaggac gaggaggagc ttgcgcacgt cgtcgagatg gagcccggtg
      481 gtgggctcgt cgaggatgaa gaggtggtcg gtggtggggc gcgcggagag ctccgccgcg
      541 atcttgagcc gctgggcctc gccgcccgag agcttcgtcg cgggctggcc cagccgcagg
      601 tagccgaggc cgacttcctg gagcaccttg agcctccgct gcaggaccgg ctccgaggcg
      661 aagaagtcca ccgcctcgtc caccgtctgc tgcagcacct ggctgatgtc gcggctcttg
      721 tacgtgacct ggagcacgtc ggggcggtag cgccggccct cgcactcctg gcacgtcacg
      781 tacacgtcct cgacgaagta catctcgagc ttctggaagc cgtcgccctc gcaggtgtcg
      841 cagcggccgc cgggcacgtt gaacgagaac gcgccgggcc ccagaccgag ggcctgggcg
      901 cggggcaggc ccgcgaagag gcgccggatc tcatcgaagg ccttcacgta cgtgaccggg
      961 ttggagcggg gcgtgcggcc gattggctcc tggtcgatga gccggatgcc cttcaagtgc
     1021 tgcagccccc gcagcgcgtc gtgggcgccc gccaccgcga agtccacctt gaagtggcgg
     1081 gccaccgcgc ggtagagcgt gtcgtgcacg agcgtggact tcccggagcc ggacaccccg
     1141 gtcacgcagg tgagggtgtg cagcgggatc cgcaccgtga cgtccttgag gttgttggcg
     1201 cgggcgccct cgaggacgag ggagcggcgg ccttcccggc gcgccagcgg cagcgggatc
     1261 gtgtctcggc cgctcaggta gcgcgcggtc agcgagcgcg ggtccttgcg gaactcggcc
     1321 tgggtgcccg cgaagaccat ggcgccgcca cgctcgcccg agccgggccc catctcgatc
     1381 acgtagtcag ccgcctcgat cagcgcgcga tcgtgctcga cgatcaccac cgtgttgccg
     1441 cccgacgcga gctcgcggca gagctcggcc agccgcgcgg tgtcccgcgc gtgcagcccg
     1501 atcgacggct cgtcaagcac gtagagcgtg ccggtgagct gggcgccgag ctgattggcg
     1561 aggttgatcc gctgcgcctc cccgccggag agcgtgcggg tctgccgccc gagggtgagg
     1621 tagccgaggc cgacgcggag caggaaggag agcttggccc gcagcatctt gagcacgtcg
     1681 cgcgccaccc cggcctccca gggggagagc gtcagcgtcg cgaagagcgc gtccaggtcc
     1741 tcgacggtcc tctccccgag ctcggcgatg ctcgcgccgc ccacgcggac cgcgagcgcc
     1801 tccggccgca ggcgccggcc ctcgcagcgc gggcacggcg actggctgcg gtagcgcgag
     1861 aggaagacgc gaacgtgaag cttgtaccgg tacgactcca cttcctcgaa gaacccgcgg
     1921 atgccggtga gcccgccgcc gccgtcgtag acccactgcc ggtcctccgc ggacagctcc
     1981 gcgtagggac ggcccacgtc gacgccgcgc ttcttggccg ccttgagcag ctgcttctgg
     2041 taccagcggc ccgaaggatg cgaccagggc tccaccgcgc cctcggccag gctctgggtg
     2101 ggatccggta ccacgcgggc ttcgtcgtag cgcagcacgt tgccgaagcc cttgcactcg
     2161 ggacacgcgc cgagcggatg gttgaacgag aagagcaggg gccgcgggcg cgccagcgcc
     2221 accccgcagg atgggcagtg gaagtcgcgg ctcaccggga cgatgccgcg ctcgaggaga
     2281 tccacgctgg cccggccctc gccctcggcg agcgcggtct cgagggaatc ggtgatgcgg
     2341 cggcgactgt ccgggccgat cacgacacga tccagcacca ccgccaggcg cgccgggtcg
     2401 agatcggcgg gcacgccgtc cgggcggccc gtcacctcgg ccaggtccag cacggcgtca
     2461 cccgctttga cccgggcgaa gccgcgctgg agcagggtgg cgaacagggc gcgcggatcg
     2521 cgggccgctt ccacgggcag gggaaagcag atgagggcgc gcgccccggg gtgatcgcgg
     2581 agcagggcgt cggccacctg gtcggccgag tcgctccgcg cctccgcccc gcaggtgggg
     2641 cagtgcacgc ggccgatctt ggcgaagagc aggcggaggt agtcgtgcag ctcggtggcg
     2701 gtgcccaccg tggagcgcgc ggtgcgcacc gggttcttct gctcgagcgc gatggcgggc
     2761 cggatgtgct cgatgcggtc cacgtcgggc cggtcgaggc gctccaggaa catgcgggcg
     2821 taggtggaca gcgactcgat gtagcgccac tggccttccg cgaagagggt gtcgaaggcc
     2881 agggaggact tgcccgagcc ggagaccccg gtgatcacgg tgacgcggtc gtgggggatg
     2941 tcgacggaga cgttcttcag gttgttctgc cgggctccct cgatccggag ccagacgggc
     3001 tgggacattc gagcgatcct cctgagatgg gaagtctacg cgacggcccg gaggccttca
     3061 atagcgcggc gcgcgcggag gcgcgcgtga cgcttgacag ccgtcgcgcg agccggtact
     3121 ctcctgatcc gtaatgagaa ccattcctaa aaggcggccg cccgcgatgg gggaaggcgc
     3181 cgacaccgcc ctgcgcgccc gcgggctccg gctcaccggg ccgcgccggg tggtgctcga
     3241 cgtggtgcgg gccaccgagt cgcatcccac cgcggagtgg gtccaccaga tggtccgccg
     3301 ccgcctgccg cgcgtgagcc tgggtacggt gtaccgcaac ctgcgcttgc tggtggccga
     3361 gggactggtc aaggagctgc cgggcccgca cgcgcgcttc gacggcaacc tgagcgagca
     3421 ccaccacttc acctgcctgg cctgcggccg gatcagcgac gtgccggcgc cggccaacga
     3481 cccccactcc cgcgccctct gcggacgggt gtccgcccag agcggcggga gcgtcaccca
     3541 tcatcgaatc gagttctacg gccgctgtcc cgagtgccaa cgccgcgccg gcggccgggc
     3601 gcggagccgg cgcccgacac cgtaactcgg cgggccgcgg cccccgagtc gccaccacca
     3661 accgaacgga ggacgtcacc atggcaggaa agccgctgaa gggcagcaag agcgtggaca
     3721 atctcaagga agcctttgcg ggcgagtcgc aggccaaccg ccggtacctg tacttcgcgc
     3781 gggtcgcgga catcgagggg ttcccggacg tcgcgggact cttcaaggac accgccgacg
     3841 ccgagaccgg ccatgccttc ggtcacctcg acttcctcaa ggaagtgggc gacccggcga
     3901 ccggggagcc catcggcaag accgagaaga acctcaaggc cgccgtcgcg ggcgagacct
     3961 acgagtacac ccagatgtac ccgggcatgg ccaagaccgc gcgggacgag gggtacccgg
     4021 agctggccga gtggttcgag acgctcgcca aggccgagaa gtcgcacgcc ggccgcttca
     4081 cgaagggcct caaccaggtc gcgggcaagg agccggccga cgccatctag tcacgccgcc
     4141 ctcgatggga ctcgggccgg gcgccggcgt gggaggcggc gcccggcctc ttcatcaccg
     4201 caatcatcac tcgtgagagg acgatgacac tcgacattcg agtcccggag ttctggcagc
     4261 tcgaccgcgt ggacaccgag ctgcgccggg tctacgacgt ctgcgcgggc tgccgccgct
     4321 gcctgccgct ctgcccctcg ttcaaggtca tgttcgaccg gctggacgtg gacgcggtgg
     4381 acggcgacgt cgagaagctg cccgcggcgg acgtcaagga agtcgtcgat ctctgctatc
     4441 agtgcaagct ctgctacaac cactgcccct acacgccacc gcatcgctgg gcggtggatt
     4501 tcccgcgtct catgctgcgg gcccgctccg cggaggcgcg cgccaagggc gtgacattgc
     4561 aggaccgctt cctcggcaac accgagctgg tcgggcgcat cggcagcctc accgcgccga
     4621 tctcgaactg gatgaatgcg gtgtgggtga atcgcaagtt catggagaag gcggtcggca
     4681 tccacgcccg acgcaatctg ccgcccttcc accgggagac gttctcgcgc tggttcgacg
     4741 ggcggaagcc ccggttgcgg agcgcgccgg cgcgcgaccg cgtggccttc ttcaccacgt
     4801 gcacggtgga gtacaacgat cccgccaccg ggaaggccgc ggtcgcggtg ctcgagaaga
     4861 acggggtgga cgtgacgctg cccgcccagc gctgctgcgg gatgccctac ctcgacggcg
     4921 gcgcggtcac cgaggccaag cggttgatcg cggccaacgt caagagcctc gccgagcagg
     4981 tccggctggg gcgggagatc gtggtgccgg ggccgacgtg ctcctacatg ctcaagcagg
     5041 agtatccgtg gctcgacggc tcggacgacg ccaacctggt cgcggcgcac acgcgggatc
     5101 tcttcgagta cctttcggcg ctgcacgaga agggccggct cgacacgagc ttcccgaacg
     5161 cgccgggcac catcgcctac cagctaccct gccacctgaa ggcccagaac atgggcaccc
     5221 gctccgccga cgtgctgcgc ctgacc
//
LOCUS       DASUFA010000348         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1668992, whole genome shotgun sequence.
ACCESSION   DASUFA010000348 DASUFA010000000
VERSION     DASUFA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668992"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..679)
                     /locus_tag="VFX87_12780"
     CDS             complement(194..679)
                     /locus_tag="VFX87_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4995848.1"
                     /translation="MARRALVIRRGTERDVPTILKLIRGLAEYERLAHEMEATTARVR
                     AHGFGRRRYFETIICRRGGEPVGFALYFFTYSTFLARPTLYLEDLFVLPEERGTGAGK
                     ALLRALARIAVRRGCGRLEWAVLDWNRPAIGFYKRLGAKLRRQWILTRLTGAPLRRLA
                     R"
     gene            complement(679..2007)
                     /locus_tag="VFX87_12785"
     CDS             complement(679..2007)
                     /locus_tag="VFX87_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4995849.1"
                     /translation="MNWDALGRETVELLRQYLMLDTTNPPGNETVGARFLEDVLGRAG
                     IASETVESAPGRGNLVARLRGDGSEGGIVLHHHIDVVYADRRYWTVEPFGGEIRDGFL
                     YGRGAIDMKSTGILHLAGLLAIKRAKVPLKRDLILLATADEEAGSRFGAQFVADRHRE
                     WLIGAEYALSELGGIYPEAGSTAPFGSIVTSEKTGLPLRLTARSEPGHGSMPWPDTAP
                     NRLIRALGRLLEAERPLRVLPEVQEYFAKLGSVLPGEAGRGYDDLEGSLRDASFRRAF
                     LAKRLRAAMLRTTFAVTMLKGSEKRNVIPPEAVADIDCRMLRGDDPEEIVSWVRGVVA
                     DERVEVSLSNPPAKIPNSSPPDTPLYKALGQALARRAPGVVVAPDILVGFTDNWVFRN
                     CGLHGYGFSPFILGDDEWRRVHGNDERISLTNLSEGVRCHTEMLLDVAAA"
     gene            complement(2132..3118)
                     /locus_tag="VFX87_12790"
     CDS             complement(2132..3118)
                     /locus_tag="VFX87_12790"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995850.1"
                     /translation="MTTRSPDTGYRPPFWYRGRHLQTLWGPLLRNWRRSPLRRERLHT
                     PDGDFLDLDWLAGAPERSPLLLILHGLEGSARSHYARGLLRGAGALGWRAAVMHFRSC
                     SGEVNRLPKLYHSGETSDLEWVIGELAKREPAPRIGLVGVSLGGNVALKWLGEQGATA
                     PAEIRAAAAISTPFDLAACAGVLDRGFNRAIYTESFLRTMKAKIRAKRHLYDGRLDVD
                     AALRARTFTEYDRFFTAPINGFADERDYWTRASSAPYLERIRRPTLLINAVNDPFMPA
                     ACLPTAVVERSRWLEAAFVAEGGHVGFLDGALGRASWAERRALAFLARHLLR"
     gene            complement(3115..3348)
                     /locus_tag="VFX87_12795"
     CDS             complement(3115..3348)
                     /locus_tag="VFX87_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995851.1"
                     /translation="MLDGIVFEKVGIPAASIVTDVFEATGHAMAQSWGVPEYRFLALP
                     HPIANLSDSDLDARARAIAPEVVRLLLKGQEGH"
     gene            complement(3370..3657)
                     /locus_tag="VFX87_12800"
     CDS             complement(3370..3657)
                     /locus_tag="VFX87_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995852.1"
                     /translation="MAGFEILDPTVEPRRQPLTYVARPDSLKGKRIGLVENTKYNSDK
                     LLVRIGDILKQEYGAAETRMWRKHNAGVPAHAEIIEEAKRTVDVVVAGIGD"
     gene            complement(3657..4700)
                     /locus_tag="VFX87_12805"
     CDS             complement(3657..4700)
                     /locus_tag="VFX87_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995853.1"
                     /translation="MTTRISSQRYAVEDFNEALELCYSRGWTDGLPVVPPTEPAVRAM
                     LDAVGLEPDGQVAFITNRQVAISAEKVAINAVMAGCRPEHMPVVLAAVEGIADPRWGY
                     HGPATSTGGAGVLMIVNGPIARRLDFNCGDNLFGPGWRSNATVGRAVRLVMRNVIGTI
                     PGRLDRGTVGHPGKYTYVIAENEAESPWTPLHAERGCRPEDSAVTVMAALGPHQFYNQ
                     LSRTAAGVLGTMCDAMRYPGMVGQPNYCVVLAGEHMRTIAADGWSKSDIRKYVFENTT
                     STIAHLKRTGRLPGAIKPEDEAATRPLVLSPDDILVVAAGGRAGSFSCYIPGWASRTS
                     SEAVTTVIKERRT"
     gene            4852..>5027
                     /locus_tag="VFX87_12810"
     CDS             4852..>5027
                     /locus_tag="VFX87_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4995854.1"
                     /translation="MSTPERRPHVVIVGGGFGGLYAARALTGSWARVTLLDRRNHHLF
                     QPLLYQVATAALNPS"
BASE COUNT          705 a         1833 c         1760 g          729 t
ORIGIN      
        1 ctcgcaccag tggaacttcc cggcctcgct ctcggtgccg ccgcgcgtgc tgccgggcgg
       61 gcacgtgatg ggctgggcgg acaccggaac cgacgccgcg acgatgacgg ccaccagcga
      121 gaccccgagg gcgaagcgga cgatcattcg gcgatccacg tctcggccac ccgcagcacc
      181 gtcgaggttg ggctcagcgc gccaggcggc gcagcggggc cccggtgagc cgcgtgagga
      241 tccactgccg gcggagcttc gcgcccagcc gcttgtaaaa gccgatcgcg ggccggttcc
      301 agtcgagcac cgcccactcg aggcgcccgc acccgcgccg gaccgcgatg cgcgccaggg
      361 cccggagcag cgccttcccg gccccggtgc cccgctcctc gggcagcacg aagagatcct
      421 cgagatagag cgtgggtcgc gcgaggaacg tggagtaggt gaagaagtag agcgcgaagc
      481 ccaccggctc gccgccgcgc cggcagatga tcgtctcgaa gtagcgccgg cgcccgaagc
      541 cgtgggcccg gacccgcgcg gtcgtggctt ccatctcgtg cgcgaggcgc tcgtactcgg
      601 cgaggccgcg gatcagcttg aggatcgtgg gcacgtcccg ctcggtcccc cgccggatca
      661 ccagcgcccg gcgcgccatc acgcggccgc cacgtccagc agcatctcgg tgtggcagcg
      721 cacgccctcg ctcaggttcg tgagcgagat ccgctcgtcg ttgccgtgca cgcgccgcca
      781 ctcgtcgtcg cccaggatga aggggctgaa gccgtacccg tgcagcccgc agttgcggaa
      841 cacccagttg tcggtgaaac ccaccaggat atcgggcgcc accaccacgc ccggggcgcg
      901 ccgcgcgagc gcctggccga gcgccttgta gagcggggtg tcgggcggcg aggagttcgg
      961 gatcttggcg ggcgggttgg agagactcac ctccacccgc tcgtcggcga ccacgccgcg
     1021 tacccagctc acgatctcct ccgggtcgtc accgcgcagc atgcggcagt cgatgtcggc
     1081 gaccgcctcc ggcgggatca cgttgcgctt ctcgcttccc ttgagcatgg tgaccgcgaa
     1141 ggtcgtccgc agcatcgcgg cgcgcaggcg cttggccagg aaggcgcgcc ggaagctcgc
     1201 gtcgcgcagc gagccctcga ggtcgtcgta gccccgcccc gcctcgcccg gcagcaccga
     1261 gccgagcttg gcgaagtatt cctggacctc gggcaggacg cgcagcggac gctcggcctc
     1321 gagcagccgg cccagcgcgc ggatgagccg gttgggcgcg gtgtcgggcc agggcatcga
     1381 gccgtggccg ggctcgctcc gcgcggtgag gcggagcggc agccccgtct tctcggacgt
     1441 cacgatgctc ccgaacggcg ccgtggagcc cgcttccggg tagatgcccc ccagctcgga
     1501 cagcgcgtac tcggccccga tcagccactc gcggtgccgg tcggccacga actgggcgcc
     1561 gaagcgactc cccgcctcct cgtcggcggt ggcgagcagg atgaggtcgc gcttgagcgg
     1621 caccttcgcg cgcttgatcg cgaggagccc ggccaggtgc aggatgccgg tggatttcat
     1681 gtcgatggcg ccgcggccgt agaggaaccc gtcgcggatc tcgccgccga agggctccac
     1741 cgtccagtag cgccggtcgg cgtagaccac gtcgatgtgg tggtgcagca cgatgccgcc
     1801 ctcggagccg tcgccgcgaa ggcgcgccac cagattgccg cggcccggcg ccgactccac
     1861 cgtctcgctc gcgatgcccg cgcgccccag tacgtcctcg agaaagcgcg ctcccaccgt
     1921 ctcgttgccg ggcggattcg tggtgtcgag catgaggtac tgccggagca gctcgaccgt
     1981 ctcgcggccc agggcgtccc agttcatgaa gtgctcgcgg gctcccggac gtggcccggc
     2041 gccgggcggc ccagcacgaa gtcgggaccg gcgtcgaccg cttgggagcc cgtcgagacg
     2101 gtcaggccgt acgcgtcacg aagcacgctc gtcatcgtag caaatggcgc gccaggaagg
     2161 cgagcgcgcg gcgctccgcc cacgaggcgc gcccgagcgc tccgtcgaga aaccccacgt
     2221 gtccgccctc ggcgacgaag gccgcctcga gccaccgcga tcgttccacc accgcggtgg
     2281 gcaggcacgc cgccggcatg aacggatcgt tgaccgcgtt gatcagaagc gtagggcgcc
     2341 ggatgcgctc gagatagggg gcgctgctcg cccgggtcca gtagtcgcgc tcgtccgcaa
     2401 agccgttgat cggcgccgtg aagaaccgat cgtactccgt gaaggtgcgc gcccggagcg
     2461 cggcgtccac gtccagacga ccgtcgtaga ggtgccgctt ggcgcggatc ttggccttca
     2521 tggtgcgcag gaagctctcg gtgtagatcg cgcggttgaa cccgcggtcg agcaccccgg
     2581 cgcaggcggc caggtcgaac ggcgtcgaga tggcggccgc ggccctgatc tcggcggggg
     2641 ccgtggcccc ctgctcgccg agccacttga gcgcgacgtt gccgccgagg gacacgccca
     2701 ccaggccgat gcgcggcgcg ggctcccgct tggccagctc gccgatcacc cactcgagat
     2761 ccgacgtctc gcccgagtga tagagcttcg ggagccggtt cacctcgccg ctgcacgagc
     2821 ggaagtgcat gacggcggcc cgccagccga gggcccccgc gccccggagc aggccgcgcg
     2881 cgtagtggga ccgggccgag ccctcgagcc cgtggaggat gaggagcagc gggctccgct
     2941 cgggcgcgcc ggcgagccag tccaggtcga ggaagtcgcc gtccggggtg tgcaggcgct
     3001 cccggcgcag cggggaccgc cgccagttgc gcagcagcgg cccccagagc gtctggaggt
     3061 gccggccccg ataccagaac ggaggccggt agccggtatc tggagaacga gtggtcagtg
     3121 gccttcttgg cctttcagta gcagtcggac gacttcgggc gcgatggcac gggcgcgggc
     3181 gtcgaggtcg ctgtcgctca ggttcgcgat ggggtgcggg agggccagga agcgatactc
     3241 cgggacgccc caggactgcg ccatggcgtg gccggtggcc tcgaacacgt cggtgacgat
     3301 ggacgcggcc gggatgccga ccttctcgaa cacgattccg tcgagcacac tgcccgacga
     3361 gcaggaccct cagtcgccga tgccggcgac gacgacgtcg acggtgcgct tggcctcctc
     3421 gatgatctcc gcgtgcgcgg gcaccccggc gttgtgcttg cgccacatcc gggtctcggc
     3481 ggccccgtac tcctgcttca ggatgtcgcc gatccgcacc agcagcttgt ccgagttgta
     3541 cttcgtgttc tccaccagcc cgatgcgctt gcccttgagc gagtccggcc gcgccacgta
     3601 cgtcagcggc tggcgccggg gctcgaccgt cggatccagg atctcgaagc ccgccatcat
     3661 gttctcctct ctttgatcac cgtggtcacc gcctccgacg acgtccggct cgcccagccc
     3721 gggatgtagc acgagaacga gccggcgcgg ccccccgccg ccaccaccag gatgtcgtcc
     3781 gggctcagca ccaggggccg ggtcgcggcc tcgtcctccg gcttgatcgc gccgggcagc
     3841 cggccggttc gcttcagatg ggcgatcgtg ctcgtggtgt tctcgaagac gtacttgcgg
     3901 atgtcggact tcgaccatcc gtccgccgcg atcgtccgca tgtgctcgcc cgccagcacc
     3961 acgcagtagt tcggctggcc caccatgccg gggtagcgca tggcgtcgca catggtcccc
     4021 aagacacccg cggcggttct cgagagctga ttgtagaact ggtgcggtcc cagcgcggcc
     4081 atcacggtca ccgcgctgtc ttccggccgg cagccgcgct ccgcgtgaag cggcgtccac
     4141 gggctctccg cctcgttctc cgcgatcacg tacgtgtact tgccggggtg gcccaccgtg
     4201 ccgcgatcga gacgccccgg aatggtgccg atcacgttgc gcatcaccag gcgcaccgcg
     4261 cggccgacgg tggcgttgga gcgccagccc ggcccgaaca ggttgtcgcc gcagttgaag
     4321 tcgagccgcc gcgcgatggg cccgttgacg atcatcagca cgcccgcgcc gccggtggag
     4381 gtggccggcc cgtgatagcc ccagcgcggg tcggcgatgc cctcgaccgc ggccagcacc
     4441 accggcatgt gctcgggccg gcagcccgcc atcaccgcgt tgatcgccac cttctcggcg
     4501 ctgatcgcca cctggcggtt cgtgatgaag gccacctgcc cgtcgggctc aagcccgacg
     4561 gcgtcgagca tcgcgcgcac cgccggctcc gtgggcggga cgaccggcag cccgtcggtc
     4621 cacccccggc tgtagcagag ctcgagcgcc tcgttgaagt cctcgacggc gtaccgctgg
     4681 gagctgatgc gcgtggtcat gccttcatcc tcggtcgttg cggttcacgg cgcgatccct
     4741 gttccgggcc ccactcttcg ggccccactt ttcggtccga ggtcacccta gggccaaccc
     4801 cacgtcctgt caagggttga cacctcccgg acgccctcgg aagatggccc cgtgagcacc
     4861 ccggagcggc ggccgcacgt ggtcatcgtc ggcggcggat tcggcgggct ctacgccgcg
     4921 cgcgccctca cggggagctg ggcgcgggtc accctcctcg atcgacgcaa ccatcacctc
     4981 ttccagcccc tgctctacca ggtcgccacc gccgcgctca atccgtc
//
LOCUS       DASUFA010000349         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_520485, whole genome shotgun sequence.
ACCESSION   DASUFA010000349 DASUFA010000000
VERSION     DASUFA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520485"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFX87_12815"
     CDS             <1..541
                     /locus_tag="VFX87_12815"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4995855.1"
                     /translation="DAHARISADRDAAVAFLRKQGFAEAEIERQPTRTVDKLAREYGQ
                     PQAAERLRYVVTTSVVVKTLNVELVRTAVGATEELLKAGVILDGEREGAANPRYVVSR
                     FNDLRPQLLADATKNARATAQQFAADSGAQVGVIRSANQGSIQIFGMDGNDESGPFSQ
                     TSTPMKRIRVVSTFEFELR"
     gene            558..1754
                     /locus_tag="VFX87_12820"
     CDS             558..1754
                     /locus_tag="VFX87_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4995856.1"
                     /translation="MRARLAASAISLALATVVAGTHGGCAAMHRPPATPASTVAGGEA
                     TGAAHEATTARLIDGLAGRAVARGDRTLDLLLLSGGGQHGAYGIGFLRGWRGRAAAPM
                     PRFDLVTGIGTGALQAPFALLGTEAALDRAATVYRNLATGSAPTGDWLFWLRRTGGVV
                     DTARYRRAIATEILSERTRDELWVELEAGRQLATATTDLDLGIGRVWDLGREIRSGAG
                     GAERVQAVLLATTAIPGTFPSVVIDGHVHADGGLVSNVLPVLDFEGARRLAARLRALG
                     VKEPVTVRVWVVMNLWTHPRPIVTDPASRSQIADRGNLLLFWAQQPQIPERLADLARA
                     VSAGVPGLRMEVRYTAIPSELAAEPGAASVADEAWMRRLEKIGNDRALGATPWDEITS
                     PYRRPR"
     gene            1809..2243
                     /locus_tag="VFX87_12825"
     CDS             1809..2243
                     /locus_tag="VFX87_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4995857.1"
                     /translation="MRVGETADLGSCAITEAEILAFARKYDPQPFHTDLEAARRSIYG
                     GLIASGWHTCALMMRLSVDAARRDEAATTGSPGLDSCRWLKPVRPGDVLSARTEVLET
                     WPSRTKPIGFVRRRSEMVNQQGEVVLRVIGITMYRRRPAAAA"
     gene            2240..2677
                     /locus_tag="VFX87_12830"
     CDS             2240..2677
                     /locus_tag="VFX87_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4995858.1"
                     /translation="MKYFEDVQVGDVMRFGRYEVTREEIVEYARQFDPQPFHLDEETA
                     RASMFGGLIASGWHTGAMFIRMVVEHMTPGHATSGAMGFDDLKWIRPVRPGDVLSVES
                     EIREKIESRSRPDRGTVKIESRISNQRGEVVMSLVSLVIYLRR"
     gene            2683..3240
                     /gene="nbaC"
                     /locus_tag="VFX87_12835"
     CDS             2683..3240
                     /gene="nbaC"
                     /locus_tag="VFX87_12835"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX4995859.1"
                     /translation="MRALVAFNLWQWIDQNRATFEPPVGNKVIWENSQFIAMVVRGPN
                     ARHDFHVDPSDEIFYMLRGAMRLEYMVDGRRHEQVIREGELCLLPALVPHSPHRPADT
                     WGLVIEVKRRPEQSESLLWFCDRCDRLLHEVTMHVADIERELKAAIEHFDASLALRTC
                     RGCGHVHPARAPLVEPVTKTLAGDL"
BASE COUNT          547 a         1283 c         1363 g          507 t
ORIGIN      
        1 ggacgcgcac gcgcgcatca gcgcggaccg cgacgcggcg gtggccttct tgcgcaagca
       61 gggcttcgcg gaggcggaga tcgagcggca gcccacccgc accgtggaca agctcgcccg
      121 ggagtacggc cagccgcagg cggccgagcg gctccgctac gtggtgacga cctcggtggt
      181 cgtgaagacc ctcaacgtgg agctggtgcg gaccgcggtc ggtgccaccg aagagctgct
      241 caaggcgggg gtgatcctgg acggcgagcg cgagggcgcg gccaacccgc gctacgtggt
      301 ctccaggttc aacgatctgc ggccccagct cctggccgac gccaccaaga acgcccgcgc
      361 gaccgcccag cagttcgcgg ccgactcggg cgcgcaggtg ggcgtcatcc gctccgccaa
      421 ccagggctcg atccagatct tcgggatgga cggcaacgac gagtcggggc cgttcagcca
      481 gacgagcacg ccgatgaagc gcatccgggt ggtcagcacc ttcgagttcg agctgcgcta
      541 ggaggatgga gatgagaatg cgcgcacgcc tggcagcctc cgccatctcg ctggccctgg
      601 ccacggtcgt cgcggggacg cacggcggct gcgccgccat gcaccgcccg cccgccacgc
      661 cggcctccac cgtggcgggc ggggaggcga cgggggccgc gcacgaggcg acgacggccc
      721 ggctgatcga cggcctggcc ggtcgcgctg tcgcccgcgg ggaccgcacg ctcgatctgc
      781 tcctgctgtc ggggggcggc cagcacgggg cctacggtat cgggttcctc cgaggctggc
      841 gcggccgggc cgccgcgccg atgcctcgat tcgacctcgt caccggcatc ggcaccggcg
      901 cgctgcaggc gccgttcgcg ctcctcggca ccgaagcggc gctggatcgc gcggcgacgg
      961 tctaccgcaa cctggccacc gggtccgccc cgacggggga ctggctcttc tggcttcggc
     1021 gcaccggcgg cgtcgtggat accgcccggt accggcgggc catcgccacc gagatcctga
     1081 gcgagcgcac gcgcgacgag ctgtgggtcg agctcgaggc gggccggcag ctcgccaccg
     1141 cgaccaccga tctcgacctg ggcatcgggc gcgtctggga cctgggccgg gagatcagat
     1201 ccggcgcggg cggcgcggag cgggtgcagg cagtgctcct ggccaccacc gcgatcccgg
     1261 gcaccttccc atcggtggtg atcgacggcc acgtgcacgc cgacgggggc ctggtctcga
     1321 acgtgctccc ggtgctcgat ttcgaaggcg ctcggcggct ggcggcgcgg ctgcgggcgc
     1381 tcggcgtgaa ggagccggtc acggtacgcg tctgggtggt tatgaacctg tggacgcacc
     1441 cgcggccgat cgtgacggat ccggccagcc gaagccagat cgccgaccgc gggaacctgc
     1501 tgctcttctg ggcgcagcag ccccagatcc cggagcggct ggccgacctg gcgcgtgcgg
     1561 tgagcgcggg cgtcccgggg ctccgcatgg aggtgcgcta caccgcgatc ccatccgagc
     1621 tggccgccga gccgggggcc gcctcggtgg ccgacgaggc gtggatgcgg aggctcgaga
     1681 agatcggcaa cgatcgcgcc ctcggcgcga caccgtggga cgagatcacc tcgccgtatc
     1741 gccggccgcg ctgagaagcg cccgcggccg gattaggaaa ggagatcggg ttgaggtact
     1801 gggaggacat gagggtgggc gagaccgccg atctcggctc gtgcgcgatc acggaggcgg
     1861 agatcctcgc gttcgcccgg aagtacgatc cgcagccgtt tcacaccgac ctggaggcgg
     1921 cgcggcggag catttacggc gggctcatcg cgagcgggtg gcacacctgc gcgctcatga
     1981 tgaggctctc ggtggacgcg gcccggcgcg acgaggcggc gaccaccggc tcgcccggcc
     2041 tcgattcctg ccgctggctc aagccggtcc ggccggggga cgtgctgagc gcgcgcaccg
     2101 aggtcctcga gacctggccc tcgcggacga agccgatcgg cttcgtccgc cggcgcagcg
     2161 agatggtgaa ccagcagggc gaggtcgtgc tgagggtgat cgggatcacg atgtaccggc
     2221 ggcgcccggc ggccgcggcg tgaagtactt cgaggacgtg caggtcggcg acgtcatgcg
     2281 cttcggccgt tacgaggtca cccgggagga gatcgtcgag tacgcccggc agttcgaccc
     2341 gcagcccttc catctggacg aggagacggc cagggccagc atgttcggcg ggctcatcgc
     2401 gagcggctgg cacaccgggg ccatgttcat ccgaatggtg gtggagcaca tgacgcccgg
     2461 gcacgccacc tccggagcca tgggcttcga cgatctcaag tggatccggc cggtgcgccc
     2521 gggcgacgtg ctctccgtgg agtcggagat ccgcgagaag atcgagtccc gaagccggcc
     2581 cgaccggggt accgtgaaga tcgagagccg gatcagcaac cagcgcgggg aggtggtgat
     2641 gagcctcgtc tcgctggtga tctacttgcg gaggtgatct gcgtgcgagc cctcgtcgcc
     2701 ttcaacctgt ggcagtggat cgatcagaac cgggccacgt tcgagccgcc ggtcggcaac
     2761 aaggtgatct gggagaactc gcagttcatc gcgatggtcg ttcgcgggcc gaacgcgcgc
     2821 cacgacttcc acgtggaccc atccgacgag atcttctaca tgctgcgagg cgcgatgcgc
     2881 ctcgagtaca tggtggatgg ccggcggcac gagcaggtca tccgtgaggg cgagctgtgc
     2941 ctgctgccgg cgctcgtccc gcactcgccc caccgcccgg ccgacacctg gggcctggtg
     3001 atcgaggtca agcgccggcc cgagcagtcc gagtccctcc tctggttctg cgaccggtgc
     3061 gatcggttgc tccacgaggt gacgatgcac gtggccgaca tcgagcggga gctcaaggcg
     3121 gccatcgagc acttcgacgc cagcctcgcg ctgcgcacgt gccggggctg cgggcacgtc
     3181 cacccggctc gggcgccgct cgtggagccg gtcacaaaga cgctggcggg agacctctag
     3241 gactcaccga ctctccgccc ccggacagga gcccgcatct tcgtcgccgt ccggctcaca
     3301 ggcggcgctg cggtgaccgc cgcccttccc cgcactcgcc ccaggagctc gacgccatgt
     3361 cggtagaagg cgacgtaggg ctcgacctct tcccaatcca ggcccgcctg gaccagcgcg
     3421 tggcccaccg cctcggcgga gggcggctgg cgcccggtga catagatgat gaagtcgtac
     3481 aggcggggcc actcagccgc ctcgggctgc ggccgcgtcc ggtcgagatg ggtcgcgaac
     3541 tccctgagca gccgctgtgc ctccgcgtcc atgatccccg ctaggacgcg cgcgcccgca
     3601 gccgccagca cgagcacagc tcacccagcg ggcggaaccg cacgggcggc tgcggaagcc
     3661 ggtccaggag ctggcatgcc ttgtcgcagc gcacgtcgtc
//
LOCUS       DASUFA010000350         7268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1669380, whole genome shotgun sequence.
ACCESSION   DASUFA010000350 DASUFA010000000
VERSION     DASUFA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669380"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VFX87_12840"
     CDS             complement(<1..1431)
                     /locus_tag="VFX87_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4995860.1"
                     /translation="MPRPTSRTLPDLLDELAARQPGHELIVGGGERLSYAETRDRARK
                     LAKGLRRLGVGAGDRVALVMTNRPEWLLIDFAVTMLGATLVPISTWSRPRELEYVLAH
                     CGVSTLITIPRLGAQRFLDAVGEMGGPGGARLPDLRRMVVAGDERPAGTIALHDLAAL
                     GRDVPDAEIDAAQRAVTPETVACILYTSGTTSTPKGVQLCHGGLIENMWNIGERQRLT
                     AADRMWMGISLFWSFGCANALVAVMSHGGTLVLQEQLDAGEALALIERERCTVYYGTP
                     NIALALTEHPDRPRRDLSSLRTGAAIGPPAAMQMVMDLGAREICNVYGLTECYGNCTV
                     TDAHDPAEVRRTTVGYPLPGMDIRVVDRESRQPLPAGEIGEILVRGHLTPGYYRDPEK
                     NAASFDGDGFLLTGDLGFIGDDGRLRFRGRIKEMVKTGGINVAPLEVEEVLLGHPAVE
                     QAYVVGLPDPRKEEILVAVVVAKPGRD"
     gene            complement(1441..1962)
                     /locus_tag="VFX87_12845"
     CDS             complement(1441..1962)
                     /locus_tag="VFX87_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4995861.1"
                     /translation="MLVEAKLATLGLSLPDLDQQYRGNASGARFISHLAVGNMLYLSG
                     TTPVKDGRPYLPGIVGADLSLEQGYEAARHAAITSLSAIKYALGDLDRVQQAVQLIGF
                     VNSAPGFSQQPRVINGATDLLVELYGDRGKPTRAAIGCQGLAINHSVEIVLTVLFAGE
                     GTRPPLARDHYAQ"
     gene            complement(1978..2787)
                     /locus_tag="VFX87_12850"
     CDS             complement(1978..2787)
                     /locus_tag="VFX87_12850"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX4995862.1"
                     /translation="MSETVTSQSETLLMEKDGPIGWITFNQPHKRNAVSQEMWQRMPE
                     YVRDLSADSAIRVVILRGAGDAAFVAGADISQFKDRRRNAADEEEYRRISAAGSESLA
                     KLGKPLLAMIHGFCIGGGVSIAITCDLRIAADDARFGIPAARLGLGYHYRGMEKLMGL
                     IGPAYTKELFFTARTDFSAQDALRMGLVNQVVPKSDLERFTRDYALMMSRNAPLTLRS
                     AKASVEQLLRPAADRDLAMLDRLITDCFNSQDYQEGVKAFSEKRRPQFQGR"
     gene            2918..4543
                     /locus_tag="VFX87_12855"
     CDS             2918..4543
                     /locus_tag="VFX87_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4995863.1"
                     /translation="MSRPTHRDACQAFRWEESARAMGFTAGPSVNLGRAIVDRHAASG
                     RAALRWFGKRGEARAYTFDELARLTARFANALRELGVGSGDRVAGFLPRIPEMLIAMI
                     GTWKAGAVYVPIFTGFGPDAIEFRVRHSGARVLVTQWEQRGRLPEPLPDGVAVVTVAG
                     GGAGAPGDVDFWRIMHGQPETTAPADSRRDEPAVLLYTSGSTGPPKGVKIAANFLVAI
                     HPHLVHGADLRADDVFWPTGDPGWGYGLVCYMGALALGVPVLAHEAAPTPEFCLARLR
                     ELGVTNLATTPTLLRGVMALGAASAGGGALRVRSASSCGEPLNAEVVRFFREHWGVTV
                     RDQYGSSEFGLPIGNFATVEMTVKPGSMGLPLPGCEIAVVDDAGREVGPGVAGHVGMK
                     PHPEGYYSLGYWSDEDRDREMYRGEWMTCGDLARRDAEGYFWFEGRADDVIKSAGYRI
                     GPFEVESAILKHPAVAEAAVVGKPDALRGQIVKAYVVLKPGRVAQRGLETEIVDVVKT
                     HVGRHQYPREIEFLDRLPKTETGKIQRFLLRGR"
     gene            4582..5022
                     /locus_tag="VFX87_12860"
     CDS             4582..5022
                     /locus_tag="VFX87_12860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4995864.1"
                     /translation="MPAYEVITGPSPWPARYTFSPAVRAGNLLFISGMTAGDESGTIV
                     GPGDIAAQTRYIFEKFAKLLAAAGGGFEHIVQTTEYVTSTENYGKTAEVRRDFFKGRF
                     PTATGVVVAGLLREGALIEISAIAVLPDLPPLPRGRGQSEGEKP"
     gene            5019..5486
                     /gene="merB"
                     /locus_tag="VFX87_12865"
     CDS             5019..5486
                     /gene="merB"
                     /locus_tag="VFX87_12865"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEX4995865.1"
                     /translation="MTDTRLMDQAFQRIMRSLVDRGRAPHYAELARVLGLPAEEGRGL
                     LRDVMQAYPIGWLHPETDYIASFPPLNNLPTQYRITVRGEQRWFAQCGFEATSATWLF
                     PGETVRVDAPCLDCGDPVTVEMRDGRITWVDPPGVVGHLAFGFGPSRGRSYYL"
     gene            5493..5741
                     /locus_tag="VFX87_12870"
     CDS             5493..5741
                     /locus_tag="VFX87_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995866.1"
                     /translation="MNLFRSEEHARRWSQFEPRSEAGFITLAELAGFFGTESRRHMLD
                     GDYLSSWYPRRAAERRAYLERIGKTSPFWVGTPDPPAS"
     gene            5798..6760
                     /locus_tag="VFX87_12875"
     CDS             5798..6760
                     /locus_tag="VFX87_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX4995867.1"
                     /translation="MKAIRVSEYGGPAVLKLEEIPTPQPGPNQVLVRNHAVGINPVDT
                     YLRSNTDNRGPKLPYTPGADAAGVIEAAGAGVTAVKRGDRVYVGGTVTGAYAEYAVCE
                     QGQVHPLAANATFAQGAAVNIPYATAYHALFNRARGEAGETVLVHGASGGVGIGAVQL
                     ARARGLTVIGTASTEKGRRLVAEQGAHHVLDHTAAGYLDECARLTGGRGPDIIMEMLA
                     NVNLQKDLNAIALRGRIVVIGNRGNTEINARIAMNKDAAILGMALNHATPAQLAGIHA
                     ALVEGLRNGSLRPVIALELPLAQAARSHEAVMEAGHHGKVVLVP"
     gene            complement(6810..>7268)
                     /locus_tag="VFX87_12880"
     CDS             complement(6810..>7268)
                     /locus_tag="VFX87_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4995868.1"
                     /translation="LCLTVGTTLRGPGVTTERAQAALSAMAPAFEIIEKRGDFAADLN
                     VSLADNAQQRAYVTGAAVPVPAGFDLAAVTLELSVNGGAPDRATGAEVLGTPAASIAW
                     LANKLAELGLALEAGMKVMSGSFTRQYPLSRGDRIEATFTPLGPVRAEFV"
BASE COUNT         1023 a         2573 c         2623 g         1049 t
ORIGIN      
        1 gtcgcggccc ggcttcgcca ccaccaccgc gaccaggatc tcctcctttc gtggatcggg
       61 caggcccacc acgtaggcct gctcgaccgc ggggtggccc agcagcacct cttcgacctc
      121 gagcggggcc acgttgatgc cgccggtctt gaccatctct ttgatccgcc cgcggaagcg
      181 cagccgcccg tcgtcgccga tgaagcccag gtccccggtg agcaggaagc cgtcgccatc
      241 gaacgacgcc gcgttcttct cgggatcgcg gtagtagccg ggcgtgaggt gcccgcgcac
      301 caggatctcg ccgatctcgc ccgcgggcag cggctgccgg ctctcgcgat ccaccactcg
      361 gatgtccatg ccgggcaggg gataacccac cgtggtccgc cgcacctccg cgggatcgtg
      421 ggcgtcggtg accgtgcagt tgccgtagca ctcggtgagc ccgtagacgt tgcagatctc
      481 gcgcgcgccc aggtccatca ccatctgcat ggccgcgggc gggccgatgg ccgccccggt
      541 gcgcagcgag gagagatcgc gccgcggccg gtcgggatgc tcggtgagcg ccagcgcgat
      601 gttcggcgtg ccgtagtaca ccgtgcagcg ctcgcgctcg attagggcca gggcctcccc
      661 ggcgtcgagc tgctcctgca acaccagcgt cccgccgtga ctcatcaccg ccaccagcgc
      721 gttggcgcag ccgaagctcc agaagaggga gatgcccatc cacatgcgat ccgccgcggt
      781 caggcgctgc cgctcgccga tgttccacat gttctcgatg agcccgccgt ggcagagctg
      841 cacgcccttg ggcgtcgagg tggtgccgga ggtgtagagg atgcaggcga ccgtctccgg
      901 cgtcaccgcc cgctgcgcgg cgtcgatctc cgcgtccggc acgtcgcgcc ccagcgcggc
      961 caggtcgtgg agcgcgatcg tccccgccgg gcgctcatcc cccgccacca ccatgcggcg
     1021 caggtcgggc aggcgggcgc cccccgggcc gcccatctcg cccaccgcgt cgaggaagcg
     1081 ctgggcgccc aggcggggga tcgtgatcag ggtgctgacg ccgcagtggg cgagcacgta
     1141 ctcgagctcg cgcggccgcg accacgtgct gatcggcacc agggtggcgc ccagcatcgt
     1201 caccgcgaag tcgatcagca gccactccgg ccggttggtc atgaccagcg cgacccgatc
     1261 gcccgcgccg acgccgagcc ggcgcagccc cttggcgagc ttccgcgccc ggtcgcgcgt
     1321 ctccgcgtag ctgagccgct ctccgcctcc cacgatgagc tcgtgacccg gctggcgcgc
     1381 ggccagctcg tcgagtagat cggggagggt ccggctcgtc gggcgcggca cgccgctccc
     1441 ctactgggcg tagtgatcgc gggcgagcgg cggcctggtg ccctcgccgg cgaacagcac
     1501 ggtgagcacg atctcgacgg agtggttgat ggcgaggccc tggcagccga tggcggcgcg
     1561 ggtcggcttg ccccggtcgc cgtagagctc gaccagcaga tcggtggcgc cgttgatcac
     1621 gcgcggctgc tggctgaagc cgggcgcgga gttgacgaag ccgatgagct gcaccgcctg
     1681 ctgcacgcgg tcgagatcgc cgagcgcgta cttgatggcg gagaggctcg tgatcgcggc
     1741 gtggcgcgcg gcctcgtagc cctgctcgag cgagagatcc gcgcccacga tcccgggcag
     1801 gtagggccgg ccgtccttca ccggcgtggt gccggagagg tacagcatgt tgccgaccgc
     1861 caggtgcgag atgaagcgcg cgccggaggc attgccgcgg tactgctgat ccagatcggg
     1921 cagggacagg ccgagcgtgg cgagcttcgc ctcgacgagc atggcggccg atcccggtca
     1981 gcggccctgg aactggggcc ggcgcttctc cgagaaggcc ttcacgccct cctggtagtc
     2041 ctggctgttg aagcagtcgg tgatcagccg atccagcatg gccaggtcgc ggtcggcggc
     2101 cgggcgcagg agctgctcca cgctggcctt ggccgagcgc agggtgagcg gggcgttgcg
     2161 cgacatcatc agcgcgtagt cccgggtgaa gcgctccagg tccgacttcg gcaccacctg
     2221 gttcaccagc cccatgcgca gggcgtcctg ggcgctgaag tcggtgcggg cggtgaagaa
     2281 cagctccttc gtgtacgcgg ggccgatcag gcccatcagc ttctccatgc cgcggtagtg
     2341 gtagcccagg cccaggcggg cggccgggat gccgaagcgg gcatcgtcgg ccgcgatgcg
     2401 caggtcgcag gtgatcgcga tgctcacccc gccgccgatg cagaagccgt ggatcatggc
     2461 gagcagcggc ttgccgagct tggccagcga ctccgatccg gccgccgaga tgcgccggta
     2521 ctcttcttca tcggccgcgt tccgccggcg atccttgaac tgggagatgt cggcgccggc
     2581 cacgaaggcg gcgtcgcccg ccccgcgcag gatgaccacc cggatcgcgc tgtcggcgga
     2641 gaggtcgcgc acgtactcgg gcatgcgctg ccacatctcc tggctcaccg cgttgcgctt
     2701 gtgcggctgg ttgaaggtga tccagccgat cgggccgtcc ttctccatca gcagcgtctc
     2761 ggattggctc gtcacggtct cgctcatggg cggtctctcc tggtctcggg gtgccgttcg
     2821 ccccctggtg tatcccgtcc gcccgtttcg cgcaagtgcg gtatccccaa gtgcggtatc
     2881 cccaagtgcg gtatcctcgg tcgccgcacc ccggaccatg agccgcccga cccatcgcga
     2941 cgcgtgccag gcctttcgct gggaggagag cgcccgggcc atgggcttca ccgcgggccc
     3001 gtcggtcaat ctcgggcgcg cgatcgtgga ccgccacgcc gcctccggcc gcgccgcgct
     3061 gcgatggttc ggcaagcgcg gtgaggcccg ggcctacacc ttcgatgagc tcgcgcggct
     3121 gaccgcccgg ttcgcgaacg cgctgcggga gctgggcgtc ggctccggcg accgggtcgc
     3181 gggcttcctg ccgcgcattc cggagatgct gatcgcgatg atcggaacgt ggaaggcggg
     3241 cgcggtctac gtgccgatct tcaccggctt cgggcccgac gccatcgagt tccgggtgcg
     3301 ccacagcggg gcccgggtgc tcgtcacgca gtgggagcag cgcgggcggc ttcccgagcc
     3361 ccttccggat ggagtcgcgg tcgtcacggt ggccggcggt ggcgcgggcg ctcccggcga
     3421 cgtggacttc tggcggatca tgcacgggca gccggagacg accgcgccgg ccgactcccg
     3481 gcgcgacgag cccgcggtgc tgctctacac ctcgggctcg accggcccgc cgaagggcgt
     3541 gaagatcgcc gcgaacttcc tggtggcgat ccatcctcat ctcgtgcacg gcgccgatct
     3601 gcgggccgac gacgtcttct ggccgaccgg cgatcccggc tggggctacg ggctggtctg
     3661 ctacatgggc gcgctcgccc tgggcgtacc cgtcctcgcg cacgaggccg cgcccacgcc
     3721 ggagttctgc ctggcgcggc tgcgcgagct gggcgtgacc aacctcgcga cgactccgac
     3781 gctgctgcgc ggggtgatgg ccctcggcgc cgcctcggcg ggcggcgggg cgctgcgcgt
     3841 gcgcagcgcg agcagctgcg gcgagccgct gaacgcggag gtggtccgct tcttccgcga
     3901 gcactggggc gtcacggtcc gcgaccagta cggctcgagc gagttcgggc ttccgatcgg
     3961 caacttcgcc accgtcgaga tgacagtcaa gccgggctcg atgggcctgc cgctgcccgg
     4021 ctgcgagatc gcggtggtgg acgacgccgg gcgcgaggtg ggcccgggcg tggccgggca
     4081 cgtggggatg aagccgcacc cggagggcta ctactcgctc ggctactgga gcgacgagga
     4141 ccgggaccgt gagatgtacc gcggagagtg gatgacctgc ggcgacctgg cccggcgcga
     4201 cgcggagggc tacttctggt tcgagggccg cgccgacgac gtgatcaaga gcgcgggcta
     4261 ccgcatcggc cccttcgagg tcgagagcgc gatcctgaag cacccggcgg tggccgaggc
     4321 cgcggtagtg ggcaagcccg acgcgctgcg gggccagatc gtcaaggcct acgtcgtcct
     4381 caagccgggc cgcgtggcgc agcgcgggct cgagaccgag atcgtcgacg tggtgaagac
     4441 ccacgtgggc cggcaccagt acccgcggga gatcgagttt ctcgaccggc tcccgaagac
     4501 cgagaccggc aagatccagc ggttcctgct gcgggggcgg tgactattca tgatggtggg
     4561 cccacgcccg aggagagtcc gatgccagcc tacgaggtga tcacggggcc gagcccctgg
     4621 cccgcccgct acacgttctc gcccgccgtc cgcgcgggca acctgctctt catctcgggc
     4681 atgaccgcgg gggacgagag cggcacgatc gtcgggcccg gcgacatcgc ggcgcagacc
     4741 cgctacatct tcgagaagtt cgcgaagctc ctggccgcgg cgggcggcgg cttcgagcac
     4801 atcgtgcaga ccaccgagta cgtcaccagc accgagaact acggcaagac cgccgaggtg
     4861 cgccgcgact tcttcaaggg ccggttcccg accgcgaccg gcgtcgtcgt cgccggcctc
     4921 ctgcgcgagg gcgcgctcat cgagatctcg gcaatcgccg tgctgcccga tcttccccct
     4981 ctccccaggg ggagagggca gagtgagggg gagaagccat gaccgatacg cgactgatgg
     5041 atcaagcgtt ccaacgcatc atgcgaagcc tggtcgaccg gggtcgcgcc ccgcactacg
     5101 ccgagcttgc gcgggtcctc gggctgcccg ccgaggaagg acgcggcctg ctccgtgacg
     5161 tcatgcaggc gtacccgatc ggttggctcc atcccgagac cgactacatc gcctcgttcc
     5221 cgcctctgaa caacctgccc acccagtacc ggatcacggt gcggggcgag cagcgctggt
     5281 tcgcccagtg cggattcgag gccacgtccg cgacgtggct gttcccgggc gagaccgtcc
     5341 gcgtggacgc gccgtgcctc gactgcggcg atccggtcac ggtggagatg cgcgacgggc
     5401 gcatcacgtg ggtggatccg cccggggtcg tcggccatct cgccttcggg ttcgggccgt
     5461 cgcgcgggcg atcgtactac ctctgaaccg gcatgaatct cttccggtcg gaagagcatg
     5521 cacggcgctg gagccagttc gagccgcggt ccgaggcagg cttcattacc ctggcggagc
     5581 tggccggatt cttcgggacc gagagccgcc ggcacatgct ggacggcgac tatctctcgt
     5641 cgtggtatcc gcgccgcgcc gccgagcgcc gcgcctatct cgagcggatc ggcaagacct
     5701 cgccgttctg ggtgggcacc ccggacccac ccgcttcatg aggcgaggcc tcgcatgcgc
     5761 acctgcggta tgctcggggg ccgaaaggag ccaatccatg aaagcgatcc gcgtctcgga
     5821 gtacggcggt ccggcggtcc tgaagctcga ggagatcccc acgccccagc cggggcccaa
     5881 ccaggtgctg gtgcgcaacc acgccgtcgg catcaatccg gtggacacct acctgcggag
     5941 caatacggac aaccgcgggc ccaagctgcc ctacaccccg ggcgcggatg cggcgggcgt
     6001 catcgaggcg gcgggcgccg gcgtgaccgc ggtgaagcgg ggtgaccgcg tctacgtggg
     6061 cggcaccgtg acgggcgcct acgccgagta cgcggtgtgc gagcaggggc aggtgcaccc
     6121 gctggccgcc aacgccacct tcgcgcaggg cgcggccgtg aacatcccct acgccaccgc
     6181 ctaccacgcg ctcttcaacc gcgcccgcgg cgaggccggc gagaccgtgc tggtgcacgg
     6241 ggccagcggc ggcgtgggca tcggcgcggt ccagctcgcg cgggcgcgcg gcctcaccgt
     6301 catcggcacc gcgagcaccg agaaggggcg gcgcctcgtg gcggagcagg gcgcgcacca
     6361 cgtcctcgac cacaccgcgg cgggctatct cgatgagtgc gcccggctga ccggcgggcg
     6421 cgggcccgac atcatcatgg agatgctcgc gaacgtgaat cttcagaagg atctgaacgc
     6481 catcgcgctg cggggccgca tcgtggtcat cggcaaccgc gggaacaccg agatcaacgc
     6541 gcggatcgcc atgaacaagg acgcggccat cctcggcatg gcgctcaacc acgccactcc
     6601 cgcccagctg gcgggcatcc acgccgcgct cgtcgagggc ctgcgcaacg gctcgctgcg
     6661 cccggtcatc gccctggagc tgccgctggc ccaggccgca cgcagccacg aagccgtgat
     6721 ggaagcgggc caccacggca aggtcgtcct cgtcccgtag gaccccctca ccctgccctc
     6781 tccccccgat gcgggggaga gggtgggggc tagacgaact cggcgcggac ggggccgagg
     6841 ggggtgaagg tggcctcgat ccggtcgccg cggctgagcg ggtactggcg ggtgaacgag
     6901 cccgacatca ccttcatgcc ggcctcgaga gccaggccga gctcggcgag cttgttggcg
     6961 agccacgcga tcgaggccgc gggcgtgccg agcacctccg ccccggtggc ccggtcgggc
     7021 gcgccgccgt tcacggagag ctccagcgtc accgcggcca ggtcgaagcc cgcgggcacc
     7081 ggcaccgccg cgccggtcac gtaggcgcgc tgctgcgcgt tgtcggccag gctcacgttc
     7141 agatcggccg cgaagtcgcc gcgcttctcg atgatctcga aggccggggc catggccgac
     7201 agcgcggcct gcgcccgctc ggtggtgacg ccgggtccgc gcagcgtcgt gccgaccgtg
     7261 agacacag
//
LOCUS       DASUFA010000351         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_417497, whole genome shotgun sequence.
ACCESSION   DASUFA010000351 DASUFA010000000
VERSION     DASUFA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417497"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFX87_12885"
     CDS             complement(<1..436)
                     /locus_tag="VFX87_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995869.1"
                     /translation="MRVGLGQIPEAGRRALAALAELAGPARPAWVVGGTLREILSGGA
                     AADLDLAVAGGALELGRRLAGSLDATFVVLDEARGAGRIVPRSGSAVDLVDLRAATLD
                     EDLRARDFTVNALAAPVDELLRHGGATIVDATGGLDDLHDRVV"
     gene            complement(436..1287)
                     /locus_tag="VFX87_12890"
     CDS             complement(436..1287)
                     /locus_tag="VFX87_12890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995870.1"
                     /translation="MASPPPGFRYIDVHTHLHPEWLSAAIRRWFVGRPGWDFQYGTEP
                     EWVARFLAEQDVERFVFFSYAHKPGLARELNAWLHETARRLPAGLPLGTVHPGDPDVV
                     DVAQEALDRYGFKGFKFHINVQRFHPDDPRILPVYELLLARAAVLLIHVGSAPWPNEY
                     DGFPRFERIMTMFPELKVIVAHMGQFETRRHLELMERCPNMYLDTTAAMAAQSAIYRE
                     GHGASAADVTDADLVRWQDRILFGSDFPNTPHTYDEERRPIWERPVPDAVHRKIFHDN
                     ARRLFGL"
     gene            complement(1287..>2130)
                     /locus_tag="VFX87_12895"
     CDS             complement(1287..>2130)
                     /locus_tag="VFX87_12895"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX4995871.1"
                     /translation="MDQAALLRRRRRNFRQLPSHRVRSARSALAFVEAVGLCSTFHRF
                     PEGLGCLWEAVVGRAAPRWPRRSHHDDGIGLTWELKDVLPARRQVYYGKLVKGRPLLV
                     ALDLFPAFYCLVRGHQPASAYRKEYEAGRLSLTAKRIMDSLVRESPQYTRGIRAECFM
                     LEPGKTREFERAMGELQQGLWIVKTEERYEPTFSYRWDLLERWLPDAVAAGRRLRRPA
                     ALERLLSRYLAGAVYSTPALLARLFGLPRREVEVALSALARAGRVSAPREVAGWPGRW
                     VVSA"
BASE COUNT          329 a          791 c          747 g          263 t
ORIGIN      
        1 gcacgacccg atcgtggaga tcgtcgagcc cgcccgtggc gtccacgatg gtcgcgccgc
       61 cgtggcgcag cagctcgtcc accggcgccg cgagcgcgtt caccgtgaag tcgcgggccc
      121 gcaggtcctc atcgagcgtc gccgcacgca gatccaccag atccaccgcg ctccccgacc
      181 gcggcacgat gcgcccggcc ccccgggcct catccagtac cacgaaggtc gcgtccaggc
      241 tgccggcgag gcggcggccc agctcgagcg ccccgccggc caccgccagg tcgagatcgg
      301 cggcggcgcc gccggacagg atctctctca gtgtgccgcc caccacccag gcgggcctcg
      361 cgggtccggc cagctcggcg agcgcggcga gggcgcggcg ccctgcctcc gggatctgac
      421 ccagcccgac gcgcactaga gaccgaacag gcgccgcgcg ttgtcgtgga agatcttgcg
      481 gtgaaccgcg tcgggcaccg gccgctccca gatcggccgc cgctcctcgt cgtacgtgtg
      541 gggcgtgttg gggaagtcgc tcccgaacag gatgcgatcc tgccagcgca cgagatcggc
      601 gtcggtgaca tcggcggcgc tcgcgccgtg cccttcccgg tagatcgcgc tctgcgcggc
      661 catcgccgcg gtggtgtcga gatacatgtt cgggcaccgc tccatcagct cgagatggcg
      721 gcgggtctcg aactggccca tgtgggccac gatcaccttg agctccggaa acatggtcat
      781 gatccgctcg aagcgcggga agccgtcgta ctcgttgggc cacggcgcgc tccccacgtg
      841 gatcaggagc accgccgcgc gggccagcag gagctcgtag accggcagga tgcgcggatc
      901 gtcgggatgg aaccgctgca cgttgatgtg gaacttgaag cccttgaacc catagcggtc
      961 gagcgcctcc tgggcgacgt ccaccacgtc gggatctccc gggtgcaccg tgcccagcgg
     1021 cagccccgcg ggcagccgcc gggccgtctc gtgaagccac gcgttcagct cgcgcgcgag
     1081 gcccggcttg tgcgcgtacg agaagaagac gaagcgctcc acgtcctgct cggcgaggaa
     1141 acgcgcgacc cactcgggct cggtcccgta ctggaagtcc cagcccgggc ggcccacgaa
     1201 ccagcgccgg atcgccgccg agagccactc gggatgcaga tgggtgtgga catcgatgta
     1261 gcggaagccg ggcgggggcg atgccatcag gcgctcacca cccagcgccc cggccagccc
     1321 gccacctcgc gcggcgcgct cacccgcccc gcccgcgcca gcgcggatag ggccacctcc
     1381 acctcccggc gcgggagccc gaagagccgc gccagcaggg cgggcgtcga gtacaccgcg
     1441 ccggccagat agcgagagag cagccgctcg agcgcggccg gccgccgaag acgccggccc
     1501 gccgcgaccg cgtccggcag ccagcgctcg agcaggtccc aacggtacga gaaggtcggc
     1561 tcgtagcgct cctcggtctt gacgatccag aggccctgct ggagctcccc catggcccgc
     1621 tcgaactcgc gggtcttacc cggctccagc atgaagcact ccgcgcggat cccccgcgtg
     1681 tactgcggcg actcgcgaac caggctatcc atgatgcgct tggcggtgag cgagaggcgg
     1741 ccggcctcgt attccttgcg gtaggcgctc gccggctggt ggccgcgcac gaggcagtag
     1801 aaggccggga agagatcgag cgccaccagg agcggccggc ccttcacgag cttgccgtag
     1861 tacacctggc gccgggcggg cagcacgtcc ttgagctccc acgtgagccc gatcccgtcg
     1921 tcgtgatgcg agcgacgcgg ccaccgcggc gcggcccggc ccaccaccgc ctcccagaga
     1981 cagcccagac cttccgggaa gcgatggaag gtcgagcaga ggccgaccgc ctccacgaag
     2041 gccagcgcgc tccgggcgga gcgcacccgg tgcgagggaa gctgacggaa gttgcgacgc
     2101 cggcgccgga gcagcgcggc ctggtccatg
//
LOCUS       DASUFA010000352         7876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1220736, whole genome shotgun sequence.
ACCESSION   DASUFA010000352 DASUFA010000000
VERSION     DASUFA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220736"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFX87_12900"
     CDS             <1..258
                     /locus_tag="VFX87_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX4995872.1"
                     /translation="LAPEPPAPVGDAQTVMPRLAGLSLRQAMEALAPFDVRLEITGRG
                     VVTGQTPLPGAPLVAGAMCRLQLAPPAGRAGHAALAASLRP"
     gene            263..1798
                     /locus_tag="VFX87_12905"
     CDS             263..1798
                     /locus_tag="VFX87_12905"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX4995873.1"
                     /translation="MSDTTVPTTTLLEALPDKTVLGTLPGRVTGVAYDSRKVAQGELF
                     VAISGFKQDGRRFAADAVGRGAAVIVAEGADPLPGSTTPRIIVPSSREALARLADAFH
                     GHPSRRLIVVAITGTNGKTTTSLLVEALLGAGGRPTGVIGTIQYRVGDQAETASQTTP
                     EALELQSLLARMVRVGVGGAAMEVSSHALALSRVDGIEFDVAVFTNLTQDHLDFHRTL
                     EAYRDAKARLFTLLAGSRKPYRAAVVNTDDPAGAAMLAAAAADPRVRPITFGLHGQPE
                     LRPRRWESGMDGIRLEVVSSKGPIEIVSPLVGEHNVMNLLGAIGVGLALDMEPRRIGE
                     TLSRVATVPGRFERVEAGQPFLVVVDYAHTPDALERVLTTARKLVRPGGRLGVVFGCG
                     GDRDRGKRPIMGGIAARLGDFAWVTSDNPRTERAEAILAEIETGIPAEAAGRHQSMAD
                     RRRAIEAAVAWGREGDVVVIAGKGHETYQIIGTESHDFDDREVARTALRARGGAGGRA
                     WRG"
     gene            1786..3177
                     /gene="murF"
                     /locus_tag="VFX87_12910"
     CDS             1786..3177
                     /gene="murF"
                     /locus_tag="VFX87_12910"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX4995874.1"
                     /translation="MAGLSIEDVVRGTQGALVAGDLGVPVTGVSIDSRHLGIGEAFFA
                     IRGPNRDGHAFLRDAAARGAACLVVHNLPDDLPPSVPTILVDDTTRALGRLAAYHRAR
                     FTVPVAAVTGSNGKTTTKEMMAAVLGALGPVLKPEGSFNNQWGLPLTLLRLGPEHKAL
                     ALELGANTPGEIAALAAICRPTVGAVTVVSSAHMEFFGSLDGVAEEKSALVRAIPPDG
                     AVVLNADDPRVLAMHTQSRARVLYFSTRPGADVFAAGPAEDDGEGLRFTLAAGIARRP
                     VRLHFAGRHNVVNALAAAGIGLALGLTLEQVARGLETARPAKGRCVWRRAGALRILDD
                     TYNANPTSVSAALVTLGAATDARRRVVVLGDMLELGEISESAHRDMGRAVAASGAAEF
                     VGMGRWAQTAVEAARQAGLAESHHVTTFEDTVALLLKRLAPGDAVLVKGSRGMRMERV
                     VDALVARFGGVSG"
     gene            3177..4259
                     /gene="mraY"
                     /locus_tag="VFX87_12915"
     CDS             3177..4259
                     /gene="mraY"
                     /locus_tag="VFX87_12915"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX4995875.1"
                     /translation="MLYELLVPLAKYNTAFNVFRYITFRTAMATVTALVISFLLGPWL
                     INRLRQVQQGATSVREDTPERHQKTKTGTPTMGGIGILVAILVSTLLWANLRNRYMWV
                     LMLATAGLGLIGLKDDWTKLKTRRGISAKEKFGSQIVLVGALMAFLYWWPADGFTTSV
                     VIPFFKGWLLTFGWLWIPFAILVIVGASNAVNLTDGLDGLAIGPIVTAGGAFAVIAYL
                     TGNFRAAEYLRILNVKGTGELTIFCGALVGAALGFLWFNSYPAQVFMGDVGSLALGGA
                     IGTLAVLTKSELLLPLIGGIYVVEAASVIIQVGAFKLTGKRVFKMAPLHHHYEIKGWA
                     EPKIIVRFWILSFMLALLALTTLKLR"
     gene            4271..5722
                     /gene="murD"
                     /locus_tag="VFX87_12920"
     CDS             4271..5722
                     /gene="murD"
                     /locus_tag="VFX87_12920"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX4995876.1"
                     /translation="MAITITLRRDVPYPTGIFVSGNGERGATYRQSLAGKTVSVVGLA
                     KSGVAAARLIRRLGGRVLASDSSPLEALPAEARGLERLGCALWAGGHPDAAFVGANLI
                     VVSPGVPLELPALVAMRARGVPIIGELELAWRVMEADVIAITGTNGKTTTTALTGELL
                     RTQVRPVLVGGNIGTPLAEHALEFPGDGLVVAETSSFQLDTTDRFRPRVAAILNITPD
                     HLDRHGTFERYVDAKARIFANQTPTDCVVLNADDPVTAGLGSRARGRVIWFSRLTTLT
                     HTYGVFIYDGWIVAKLNGSTERICPVTEITLRGQHNVENVLAATACALWTGMSPSSIR
                     RGIAAFRGVAHRIERVHDDRGVVYYNDSKGTNVDSTIKALESFTEPVILIAGGKGKGQ
                     DFGPLAEVARGRVRRAILIGQDRVRIRAALEPAGIPVEDAESMDDAVRRAREAAQVGD
                     VVLLSPACASFDMFRNFEHRGDVFKSVVRALLG"
     gene            5722..6849
                     /gene="ftsW"
                     /locus_tag="VFX87_12925"
     CDS             5722..6849
                     /gene="ftsW"
                     /locus_tag="VFX87_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX4995877.1"
                     /translation="MPRKLTPDLPLFGVVVTLVCVGVVMVYSASAIVAADRFHDPFFF
                     LKKQLFWAVLGLGCLWAGMLLDYRWLERLVMPLLVVSFALLVLVLVPPFGQSINGTRR
                     WFHVGPVSFQPVELAKFSLVLYLASFLTRRQEVIARFVDGLLPILLVAGGMAGLTLLQ
                     PDLGNSLALIILTLALAYMAGARVQHMAAIAGCALPVVIAAIAMKPYRWRRMVAFMNP
                     WDDPQGSGFQIIQSFLALGSGGWLGVGLGDSKQKLFYLPEPYTDFIFAIIGEELGLVG
                     ATLIVALFALLIWRGLRVGLRAPDAFGAYLGLGLTIMLATQTLVNLGVVTGALPTKGL
                     PLPFISFGGSALLMTLFSAGVLLNISQHGTGYRERFMGESA"
     gene            6849..>7876
                     /gene="murC"
                     /locus_tag="VFX87_12930"
     CDS             6849..>7876
                     /gene="murC"
                     /locus_tag="VFX87_12930"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX4995878.1"
                     /translation="MFKKYQHIHFVGIGGIGMSGIAEVLLTLGYRVTGSDARRSDTVE
                     RLERLGAKVYLGHEAAHVEGAHVVVYSSAVARDNVEVQTARQRGVPVIGRAEMLAELM
                     RLKYGIAIAGTHGKTTTTSMVAAVLGAGGFDPTVVVGGRVHGLGANARLGQGEFLVAE
                     ADESDGSFLKLSPTIAVVTTVDAEHLDHYADLDAIVTAFLTFVNKVPFYGSAVVCLDD
                     PNIQRMIPRIEKRVVTYGLEAGADLMARRLSFAEMRSEFEAVHRGKSLGPVALQVPGR
                     HNVLNALAAAAVGLDLEMPFDKIQSALASFAGVQRRFQIRGEAQGVLVVDDYGHHPAE
                     IRATLAAA"
BASE COUNT         1038 a         2786 c         2854 g         1198 t
ORIGIN      
        1 ctggctccgg agccgcccgc gccggtgggc gacgcccaga ccgtgatgcc gcgcctggcc
       61 ggcctctcgc tccgccaggc catggaggcc ctcgcgccct ttgacgtgcg cctcgagatc
      121 acgggccgcg gtgtcgtgac cggccagacg ccgctgcccg gcgccccgct cgtcgcgggc
      181 gcgatgtgcc ggctccagct cgcacccccg gccggccggg ccggccacgc cgcgttggcc
      241 gcgagccttc ggccgtgagg acatgagcga taccaccgtg cccaccacca cgctgctcga
      301 ggcgctgccg gacaagacgg tgctgggtac tctgcccggc cgggtgaccg gcgtcgccta
      361 cgattcgcgg aaggtcgcgc agggcgagct cttcgtggcc atctccggct tcaagcagga
      421 cggtcggcgc ttcgccgccg acgcggtcgg gcggggcgcc gccgtgatcg tcgcggaggg
      481 cgccgacccg ctgccgggca gcacgacgcc ccgcatcatc gtgccgtcct cgcgcgaggc
      541 cctggcgcgg ctggccgatg cctttcacgg ccatccctcc cggcggctca tcgtggtcgc
      601 catcaccggc acgaacggca agaccaccac gtcgctgctc gtggaggccc tgctgggcgc
      661 gggcgggcgg cccaccggcg tgatcggcac gatccagtat cgcgtcggcg accaggccga
      721 gacggccagc cagaccacgc ccgaggccct cgagctgcag tcgctgctgg cgcggatggt
      781 gcgcgtcggc gtcggcggcg ccgcgatgga ggtctcctcg cacgcgctcg cgctctcccg
      841 ggtggacggc atcgagttcg acgtggcggt cttcaccaac ctgacccagg accacctcga
      901 cttccaccgg acgctcgagg cctaccgcga cgccaaggcc cggctcttca ccctgctggc
      961 cgggagccgc aagccctacc gcgccgcggt ggtgaacacg gacgatccgg cgggcgcggc
     1021 catgctggcc gcggcggccg ccgatccgcg ggtgcggccg atcacgttcg gcctgcacgg
     1081 acagcccgag ctgcggcccc gccgctggga atcgggcatg gacggcatcc gtctcgaggt
     1141 cgtgtcgtcg aaggggccca tcgagattgt ctcgcccctg gtgggcgagc acaacgtgat
     1201 gaatctcctg ggggccatcg gcgtcggact cgctctggac atggagccgc gacggatcgg
     1261 cgagacgctc tcccgggtgg cgacggtgcc cggccggttc gagcgcgtgg aggccggcca
     1321 gccgttcctg gtggtggtgg actacgcgca cacgcccgac gcgctggagc gcgtgctcac
     1381 caccgcgcgc aagctggtgc gcccgggcgg gcgcctcggt gtggtgttcg gttgcggcgg
     1441 cgaccgcgac cgcggcaagc gcccgatcat gggtggcatc gcggcgcggc tcggcgactt
     1501 cgcctgggtg acctccgaca acccgcgcac cgagcgggcc gaggcgatcc tggccgagat
     1561 cgagaccggc atcccggccg aggccgcggg ccggcaccag tccatggccg atcgccgtcg
     1621 cgccatcgag gccgcggtcg cctgggggcg cgagggcgac gtggtggtga tcgcggggaa
     1681 ggggcacgag acctaccaga tcatcggcac cgagtcgcac gacttcgacg accgcgaggt
     1741 ggcacggacc gcgctgcggg cgcgcggggg agccggaggg cgggcgtggc ggggctgagc
     1801 atcgaggacg tggtccgcgg cacccagggc gccctcgtgg cgggcgacct cggcgtgccc
     1861 gtcaccggcg tcagcatcga cagccgccac ctgggcatcg gagaggcctt cttcgccatc
     1921 cgcgggccga accgcgacgg ccacgccttc ctgcgcgacg cggcggcgcg cggcgcggca
     1981 tgcttggtgg ttcacaacct gccggacgac ctgccgccct cggtgcccac catcctggtg
     2041 gacgacacca cgcgcgccct cgggcggctg gccgcgtatc accgcgcgcg attcacggtg
     2101 ccggtggcgg cggtgaccgg ctccaacggc aagaccacga ccaaggagat gatggccgcg
     2161 gtcctcggcg cgctcggccc ggtgctcaag ccggagggca gcttcaataa ccagtggggc
     2221 ctgccgctca ccctcctgcg cctcgggccg gagcacaagg cgctcgcgct ggagctgggg
     2281 gcgaacacgc cgggcgagat cgccgcgctc gccgcgatct gccggcccac ggtgggcgcg
     2341 gtgacggtgg tgtcgagcgc ccacatggag ttcttcggct ccctcgacgg ggtggccgag
     2401 gagaagagcg cgctggtgcg cgcgatcccg cccgacggcg cggtcgtgct caacgccgac
     2461 gacccgcgcg tgctcgcgat gcacacgcag agccgagcgc gggtgctcta cttcagcacg
     2521 cgtcccgggg ccgacgtctt cgcggcggga ccggccgagg acgacggcga agggctgcgc
     2581 ttcacgctgg ccgccggcat cgccaggcgg ccggtgcgcc tccacttcgc ggggcgtcac
     2641 aacgtggtca acgcgctggc cgcggccggg atcgggctcg ccctggggct caccctcgag
     2701 caggtcgccc gcgggctcga gacggcgcgc ccggccaagg gccgctgcgt ctggcggcgc
     2761 gcgggggcgc tgcgcatcct cgacgacacc tacaacgcca acccgacctc ggtgagcgcc
     2821 gcgctggtca cactcggcgc cgccaccgac gcgcggcggc gggtggtcgt cctgggcgac
     2881 atgctcgagc tgggcgagat cagcgagtcc gcgcaccgcg acatgggccg cgcggtcgcc
     2941 gcgtcgggtg cggccgagtt cgtcggcatg gggcggtggg cgcagaccgc ggtggaggcg
     3001 gcgcggcagg ccgggctcgc ggagagccat cacgtgacca cgttcgagga tacggtggcg
     3061 ctgctgctca agcggctcgc gcccggcgac gcggtgctcg tcaagggctc ccggggcatg
     3121 cgcatggagc gcgtggtgga cgcgctcgtg gcgcgcttcg gaggagtgag tggctagtgc
     3181 tctacgagct cctcgtgccg ctcgcgaagt acaacaccgc gttcaacgtg ttccggtaca
     3241 tcaccttccg gaccgcgatg gcgacggtca ccgccctggt gatctccttc ctcctggggc
     3301 cgtggctcat caaccggctg cgccaggtgc aacagggggc aacgtcggtc agagaagaca
     3361 ccccggagcg gcaccagaag accaagacgg gcacgcccac catgggcggc atcgggatcc
     3421 tggtggcgat cctggtctcg accctgctgt gggccaacct gcgcaaccgc tacatgtggg
     3481 tgctgatgct ggccaccgcg ggcctgggcc tgatcggcct gaaggacgac tggacgaagc
     3541 tcaagacccg gcggggcatc tcggcgaagg agaagttcgg ctcccagatc gtgctggttg
     3601 gcgccctgat ggcgtttctc tactggtggc cggccgacgg cttcaccacc agcgtcgtga
     3661 tcccgttctt caaggggtgg ctcctcacgt tcggctggct gtggatcccc ttcgcgatcc
     3721 tggtgatcgt gggggcctcc aacgcggtga acctgaccga cgggctcgac ggcctcgcca
     3781 tcggcccgat cgtgaccgcg ggcggggcct tcgcggtgat cgcgtacctc accgggaatt
     3841 tccgggccgc cgagtacctg cggatcctca acgtcaaggg caccggcgag ctgacgatct
     3901 tctgcggcgc gctggtcggc gcggccctcg ggttcctctg gttcaacagc tacccggcgc
     3961 aggtgttcat gggcgacgtg ggctcgctgg cgctgggcgg ggccatcggg acgctcgcgg
     4021 tgctcaccaa gtcggagctc ctgctgccgc tcatcggcgg catctacgtg gtggaggcgg
     4081 cgtcggtcat catccaggtg ggcgcgttca agctgaccgg caagcgggtc ttcaagatgg
     4141 cgccgctgca ccatcactac gagatcaagg gctgggcgga gcccaagatc atcgtccgct
     4201 tctggatcct gtcgttcatg ctcgcgttgc tggcgctcac cactctcaag cttcgctagg
     4261 gtgacatgca atggccatca cgatcacgtt gcgacgggac gttccgtatc ccacggggat
     4321 cttcgtgagc ggtaacggcg agcggggggc gacgtaccgc cagtcgctcg cgggcaagac
     4381 cgtctcggtg gtcgggctgg ccaagagcgg ggtggcggcg gcccgactga tccgccgcct
     4441 cggcggccgc gtgctcgcct ccgactcgag cccgctcgag gcgctgcccg cggaggcccg
     4501 cgggctcgag cgcctgggct gcgcgctctg ggcgggcggc catcccgacg ccgcgttcgt
     4561 gggcgcgaat ttgatagtcg tgagcccggg cgtgccgctc gagctgcccg cgctcgtcgc
     4621 gatgcgcgcc cgcggggtgc cgatcatcgg cgagctcgag ctggcgtggc gggtgatgga
     4681 ggccgacgtc atcgccatca ccggcaccaa cggcaagacc acgaccaccg ccctgaccgg
     4741 cgagctgctg cgcacccagg tgcgcccggt gctggtgggc ggcaatatcg ggacgcccct
     4801 ggccgagcac gcgctcgagt tccccggcga cgggctcgtg gtggcggaga cgtccagctt
     4861 ccagcttgac accaccgacc gcttccgccc gcgggtcgcc gcgatcctga acatcacgcc
     4921 ggaccacctg gaccggcacg ggaccttcga gcgctacgtg gacgccaagg cgcggatctt
     4981 cgccaaccag acgcccaccg actgcgtggt gctgaacgcg gatgacccgg tcaccgccgg
     5041 gctcgggagc cgcgcgcgcg ggcgcgtgat ctggttcagc cggctcacca ccctgaccca
     5101 cacctacggc gtcttcatct acgacggctg gatcgtggcc aagctcaacg gcagcaccga
     5161 gcgcatctgc ccggtcaccg agatcacgct gcgcggccag cacaacgtgg agaacgtgct
     5221 cgcggccacc gcgtgcgcgc tctggaccgg catgtcgccc tcctcgatcc ggcgcggcat
     5281 cgcggccttc cgcggagtgg cccaccgcat cgagcgggtg cacgacgacc ggggggtcgt
     5341 ctactacaac gactccaagg gcaccaacgt ggactcgacg atcaaggctc tcgagagctt
     5401 caccgagccg gtgatcctga tcgcgggcgg caagggcaag ggccaggact tcggcccgct
     5461 cgccgaggtg gcccgtggcc gcgtgcgccg cgcgatcctc atcggccaag accgcgtccg
     5521 gatccgtgcc gcgctcgagc ccgccgggat tcccgtggag gacgcggagt ccatggacga
     5581 cgcggttcgc cgggcgcgcg aggcggccca ggtgggcgac gtggtgctgc tctcgcccgc
     5641 ctgcgcgtcg ttcgacatgt tccgcaactt cgagcaccgc ggcgacgtct tcaagtccgt
     5701 ggtgcgggcg ctgctcggct gatgccccgc aagctcacgc ccgacctgcc gctcttcggg
     5761 gtggtggtga ccctcgtgtg cgtgggggtg gtgatggtct actcggcgag cgcgatcgtg
     5821 gccgcggacc gcttccacga tccgttcttc ttcctgaaga agcagctctt ctgggccgtg
     5881 ctcgggctcg gctgcctctg ggccgggatg ctgctcgact accgctggct cgagcggctg
     5941 gtgatgccgc tgctcgtcgt ctccttcgcc ctgctcgtcc tggtgctggt cccgccgttc
     6001 ggccagtcca tcaacggcac gcggcgctgg ttccatgtgg ggccggtctc cttccagccg
     6061 gtggagctgg ccaagttctc gctcgtgctc tacctggcct cgttcctcac gcggcggcag
     6121 gaggtcatcg cgcgcttcgt ggacgggttg ctcccgatcc tcctggtggc gggcggcatg
     6181 gccgggctca ccctgctgca gccggacctg ggcaatagcc tggccctcat catcctgacg
     6241 ctcgcgctcg cctacatggc gggcgcccgc gtgcagcaca tggcggccat cgcgggctgc
     6301 gccctgccgg tggtgatcgc ggccatcgcc atgaagccct accggtggcg ccgcatggtc
     6361 gccttcatga acccgtggga cgacccgcag ggcagcggct tccagatcat ccagtcgttc
     6421 ctggccctgg gctcgggcgg ctggctcggg gtggggctcg gcgactccaa gcagaagctc
     6481 ttctacctgc cggagcccta caccgacttc atcttcgcga tcatcggcga ggagctgggg
     6541 ctggtgggcg ccaccctgat cgtcgcgctc ttcgcgctcc tgatctggcg cggcctgcgc
     6601 gtcggcctgc gggcccccga cgccttcggc gcctacctgg ggctggggct caccatcatg
     6661 ctcgcgaccc agaccctcgt gaacctgggt gtggtgacgg gcgcgctccc caccaagggg
     6721 ctgccgctgc cgttcatctc cttcgggggc tcggcgctcc tgatgacgct cttttcggcg
     6781 ggcgtactcc tcaacatctc ccagcatgga accggctacc gggagcggtt catgggagag
     6841 tccgcctgat gttcaagaag taccagcaca tccacttcgt gggcatcggc ggcatcggta
     6901 tgagcggcat cgcggaggtg ctgctgaccc tgggctaccg cgtgaccggc tccgacgcgc
     6961 ggcgcagcga cacggtggag cggctcgagc ggctgggggc caaggtgtat ctgggtcacg
     7021 aggccgccca cgtcgagggc gcgcacgtcg tcgtctactc ctcggcggtg gcgcgcgaca
     7081 acgtggaggt tcagacggcg cggcagcgcg gggtgccggt gatcgggcgg gccgagatgc
     7141 tcgccgagct gatgcggctc aagtacggca tcgccatcgc gggcacccac ggcaagacca
     7201 cgaccacctc gatggtcgcg gcggtgctgg gcgcgggcgg cttcgatccc accgtggtgg
     7261 tgggtgggcg cgtgcacggg ctcggggcca acgcgcggct cggccagggc gagttcctgg
     7321 tcgcggaggc cgacgagtcc gacggctcgt tcctgaagct ctcgccgacc atcgcggtgg
     7381 tgaccacggt ggacgcggag cacctggatc actacgccga cctcgacgcg atcgtgaccg
     7441 cgttcctcac cttcgtgaac aaggtgccgt tctacggctc ggcggtggtc tgcctcgacg
     7501 atcccaacat ccagcggatg atcccgagga tcgagaagcg ggtcgtgacc tacggcctcg
     7561 aggcgggcgc cgacctgatg gcgcgccggc tctcgttcgc cgagatgcgc tcggagttcg
     7621 aggcggtgca tcgcggcaag tcgctcgggc cggtcgcgct ccaggtgccc gggcggcaca
     7681 acgtgctgaa cgcgctcgcc gccgccgcgg tggggctcga tctcgagatg ccgttcgaca
     7741 agatccagtc ggcgctcgcc tcctttgcgg gcgtccagcg ccggttccag atccgggggg
     7801 aggcccaggg cgtgctcgtg gtggacgact acggccatca cccggccgag atccgggcga
     7861 ccctcgccgc cgccaa
//
LOCUS       DASUFA010000353         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1671582, whole genome shotgun sequence.
ACCESSION   DASUFA010000353 DASUFA010000000
VERSION     DASUFA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671582"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFX87_12935"
     CDS             <1..832
                     /locus_tag="VFX87_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX4995879.1"
                     /translation="LWNSLGEKDRVHIRRNLQAHSLSQFMHGEQGALIATARIVQTVP
                     TYESKFYAATQVMDEARHVEAYSRLLHEKFKMAYPITVTLAKLLEATITDRRWDMTYL
                     GMQILIEGLALAAFQRIRDNSTNPLCAAVNAYVMQDEARHVAFGRLALRDYYPQLSDA
                     ERDEREEFVVEACYHMRDRFNQKEVWENLGLPVQECIAAVDQSDTMKQFRRRLFTRIV
                     PTVKDIGLWGNRVRRAYEDMGVMEFADTDAAAILENDNRVAEEFDAKQFVGQQLGQGA
                     "
     gene            complement(900..1598)
                     /locus_tag="VFX87_12940"
     CDS             complement(900..1598)
                     /locus_tag="VFX87_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX4995880.1"
                     /translation="MIVTRPAAERGHFDHGWLDTSHTFSFASYHDPAHMGFRTLRVIN
                     EDRVAGGRGFGAHSHNDMEIVTYVLEGALEHADSMGNRSVIVPGDVQRMSAGTGVTHS
                     EANPKPDQPVHFLQIWILPDRRGLAPGYEQKAIAPQEVRGTLRLIASRDGRAGGVTIH
                     QDVDLYVSRLEPGERVKHALAGGRHAWLQMVAGRVEMNGTALGAGDGAAVSREHALEI
                     VALEPAHFLLFDLA"
     gene            1707..2714
                     /locus_tag="VFX87_12945"
     CDS             1707..2714
                     /locus_tag="VFX87_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX4995881.1"
                     /translation="MDGIGADRERRARLRAVVISLAVSVLLLAAKYQAYRLTGSTAIL
                     SDALESIVNVVAAVFALGGIIFAGRPADRNHPYGHGKIEFFSAAFEGGLIAFAAVLIV
                     YEVVLALLRGVEVRALDLGVSIVLGAGLVNLLLGWYLIRAGRTYASLTLVADGRHVLA
                     DFYTSAGIVIGLLLVRFTGIGWLDPLVAGVVALNLLWTGFRLVRHAAGGLLDEEDTDL
                     LGRLLEVLQPHLSQGVIRIHHLRAIRAGRFHHVDAHLVVPEFWTVERSHELAEDLGER
                     VMKELGAEGEMTLHTDPCHRIYCRMCDLDDCPIRREAFSGRPRLTLEEAVQPDMPRGR
                     P"
     gene            2711..3130
                     /locus_tag="VFX87_12950"
     CDS             2711..3130
                     /locus_tag="VFX87_12950"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4995882.1"
                     /translation="MSDDLLERVRARARDNSFWRYLGVEVEEAREGWVRLRVPVRDEL
                     RNAAGAPVHGGVYAALTDMAVGGALATMHEASAGGVGQTTLDMNVSFIGASTGDVFAE
                     GRILRRGRTIAFGEATITDAAGKLVAAGRATYMILAK"
     gene            complement(3133..>4470)
                     /locus_tag="VFX87_12955"
     CDS             complement(3133..>4470)
                     /locus_tag="VFX87_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX4995883.1"
                     /translation="LGRRLLITTDHEGGRIIMLNRGVTIFPDNLTAGTAGDEAMVARQ
                     GLIEGRELRRLGVDVNFGPCLDVLTERYSPNIGIRSYGKDPALVSRLGAARIRGMQAG
                     GVSACAKHFPGKGHAPVDAHLGLPVIDSDWGDMHALHLPPFMAAMEAGVDCIMTSHPL
                     YPNLDPLPAMPATFSKLIVEDYLRGQVGYRGVIVSDDLEMGAIGALCPIGEATVRTAA
                     AGHDLLLVCHTAPAQRAAYEAVLAAYRAKTLPIRALEASMARIDALDARRPERATGGA
                     PRAEADGEPLARAMAARAVTVVAAPDPGWRPALNGRVLAIFPRLSSLADRITIEPVMQ
                     DEPRYLREAMAPHGVQPEPLVVGVEPTAEEIEHARSAAAGADATVLFLYDAHLYPSNR
                     ALLDAVQAGARKLGVVLMRDPWDAEFLAPGVAALTAYGWRRCQLDAVMARLLT"
BASE COUNT          629 a         1561 c         1624 g          656 t
ORIGIN      
        1 tctctggaac agcctcggcg agaaggaccg ggtgcacatc cggcggaacc tgcaggctca
       61 ctcgctctcg cagttcatgc acggcgagca gggcgcgctc atcgccaccg cccggatcgt
      121 ccagaccgtg cccacctacg agtccaagtt ctacgcggcg acccaggtga tggacgaggc
      181 gcgccacgtg gaggcctact cgcggctgct gcacgagaag ttcaagatgg catacccgat
      241 cacggtcacc ctggccaagc tgctcgaggc caccatcacc gaccggcgct gggacatgac
      301 ctacctcggc atgcagatcc tgatcgaggg gctcgccctc gccgccttcc agcgcatccg
      361 cgacaactcg accaacccgc tgtgcgcggc ggtgaacgcc tacgtcatgc aggacgaggc
      421 gcgccacgtg gccttcggcc gtctggccct gcgcgactac tacccgcagc tcagcgacgc
      481 ggagcgcgac gagcgggagg agttcgtcgt cgaggcctgc taccacatgc gcgaccgatt
      541 caaccagaag gaagtctggg agaacttggg cctgccggtc caggagtgca tcgcggcggt
      601 ggatcagtcg gacaccatga agcagttcag acggcgtctt tttactcgga tagtccccac
      661 cgtcaaggac atcgggctct gggggaatcg ggtgcggcgg gcctacgaag acatgggcgt
      721 gatggagttc gcggacacgg atgcggccgc gatcctggag aacgacaacc gcgtggcgga
      781 ggagttcgac gccaagcagt tcgtcgggca gcagctcggc cagggggcct agaccacccc
      841 tcaccctgca gcccctcacc ctaccctctc ccctcagggg agggggcagg gtgagggggt
      901 caggcgagat cgaacaggag gaagtgcgcg ggctcgaggg ccacgatctc gagcgcgtgc
      961 tcccggctca ccgcggcgcc gtcgcccgcg ccgagcgcgg tgccgttcat ctcgacccgg
     1021 ccggccacca tctgcagcca cgcgtgccgc ccgccggcca gcgcgtgctt gacccgctcg
     1081 cccggctcga gccgcgagac gtagaggtcc acgtcctggt ggatcgtcac cccgccggcc
     1141 cggccgtcgc gggaggcgat gaggcgcagc gttccgcgca cctcctgcgg cgcgatcgcc
     1201 ttctgctcgt agccgggggc cagcccgcgg cgatccggca ggatccagat ctggaggaag
     1261 tgcaccggct ggtccggctt cgggttggcc tcgctgtggg tcaccccggt gcccgcgctc
     1321 atgcgctgga cgtcgccggg cacgatgacc gagcggttgc ccatgctgtc ggcgtgctcg
     1381 agcgcgccct ccagcacgta ggtcacgatc tccatgtcgt tgtgcgagtg cgcgccaaat
     1441 ccgcgcccgc ccgccacgcg gtcctcgttg atgacccgta gcgtgcggaa gcccatgtgc
     1501 gcggggtcgt ggtagctcgc gaacgaaaag gtgtgcgagg tgtcgagcca gccgtggtcg
     1561 aagtggccgc gctcggcggc gggacgggtg acgatcatcg cgcggctcct ttgagtgagg
     1621 ggtgccttac acgtccatgg tacctgacac ggcaagcggg ccccgccgcg ggtatcatcg
     1681 aggccatggt ggaaggggag ctcacggtgg atgggatcgg agcggaccgc gagaggcggg
     1741 cgcggctgcg cgcggtcgtc atctcgctgg cggtgtcggt gctgctgctg gcggccaagt
     1801 accaggccta tcgcctcacc ggctccaccg ccatcctgtc cgacgccctc gagtccatcg
     1861 tcaacgtggt ggccgcggtg ttcgcgctgg gcgggatcat cttcgcgggc cgccccgccg
     1921 atcgcaacca cccctacggc cacggcaaga tcgagttctt cagcgccgcc ttcgagggcg
     1981 gcctgatcgc gttcgcagcc gtgctgatcg tctacgaggt ggtgctcgcg ctgctccgcg
     2041 gcgtggaggt gcgcgcgctg gacctcggcg tctccatcgt gctgggcgcc ggcctcgtga
     2101 acctgctgct cggctggtac ctgatccgcg cgggccggac gtacgcctcg ctcaccctcg
     2161 tggccgacgg ccgccacgtg ctcgccgatt tctacaccag cgcgggcatc gtgatcggcc
     2221 tgctcctcgt ccggttcacc gggatcgggt ggctcgaccc gctggtggcc ggggtcgtgg
     2281 ccttgaatct cctgtggacc gggttccggc tcgttcgcca cgccgcgggc ggtctcctgg
     2341 acgaggagga caccgacctg ctgggccgtc tgctcgaggt cctgcagccg cacctgagcc
     2401 agggcgtcat ccgtatccac cacctccgcg cgatccgcgc cggccgcttc caccacgtgg
     2461 acgcgcacct ggtcgttccg gaattctgga cggtggagcg ctcgcacgag ctggccgagg
     2521 atctggggga gcgcgtgatg aaggagctgg gcgcggaggg cgagatgacg ctccacaccg
     2581 atccctgcca tcgcatctac tgccggatgt gcgatctcga cgactgcccg atccgccgcg
     2641 aggcgttcag cggccggccg cggctgaccc tggaggaggc ggttcaaccc gacatgcccc
     2701 gcgggaggcc atgagcgacg atctgctgga gcgggtgcgt gcccgcgcgc gcgacaattc
     2761 cttctggcgc tatctgggtg tggaggtgga agaggcacgc gagggctggg tgcgcctgcg
     2821 cgtgccggtg cgcgatgagc tgcgcaacgc ggcgggcgcg ccggtgcacg gcggggtcta
     2881 cgcggcgctg accgacatgg cggtgggcgg cgcgctcgcg accatgcacg aggcctccgc
     2941 gggcggggtg ggccagacca cgctcgacat gaacgtgagt ttcatcgggg ccagcacggg
     3001 cgacgtcttc gcggagggcc gcatcctgcg ccgcggccgc accatcgcgt tcggcgaggc
     3061 caccatcacc gacgcggcgg gcaagctcgt cgccgccggc cgcgccacct acatgattct
     3121 cgcgaagtaa ccctacgtca gcaggcgcgc catcaccgcg tcgagctggc agcgccgcca
     3181 cccgtaggcg gtgagcgccg cgacgccggg ggccaggaac tcggcgtccc acggatcgcg
     3241 catcagcacc acgcccagct tccgcgcgcc ggcctgcacc gcgtccagca gcgccctgtt
     3301 cgacgggtag aggtgcgcgt cgtagaggaa gagcacggtg gcgtcggcgc cggcggccgc
     3361 gctgcgcgcg tgctcgatct cctcggcggt cggctccacg cccaccacca ggggctcggg
     3421 ctgcacgccg tgcggcgcca tcgcctcgcg caggtagcgc ggctcgtcct gcatcaccgg
     3481 ctcgatcgtg atgcggtcgg ccagggacga cagccgcggg aagatcgcga ggacccggcc
     3541 gttgagcgcg ggccgccacc cggggtcggg cgccgccacc accgtgaccg cgcgcgcggc
     3601 catcgcgcgt gccagcggct cgccgtcggc ctccgcgcgg ggcgcgccgc cggtggcgcg
     3661 ctcgggccgc ctggcgtcga gcgcgtcgat ccgcgccatg ctggcctcga gcgcgcggat
     3721 cggcagcgtc ttggcgcgat aggcggcgag caccgcctcg taggccgcgc gctgggccgg
     3781 cgcggtgtgg cagaccagca ggagatcgtg cccggccgcg gcggtgcgca cggtggcctc
     3841 gccgatcggg cagagcgcgc cgatcgcgcc catctcgaga tcgtccgaca cgatgacgcc
     3901 gcgatagccc acctggccgc gcaggtagtc ctccacgatc agcttggaga acgtggccgg
     3961 catggcgggg aggggatcca ggttcgggta gagcgggtgc gaggtcatga tgcagtcaac
     4021 gcccgcctcc atcgcggcca tgaacggggg caggtggagc gcgtgcatgt cgccccagtc
     4081 ggagtcgatc acgggcaggc ccaggtgcgc gtccaccggc gcgtgcccct tgccggggaa
     4141 gtgcttggcg caggccgaga cgccgcccgc ctgcatgccg cggatgcgcg cggcgcccag
     4201 gcgggagacc agcgccgggt ccttgccgta ggagcggatg ccgatgttcg ggctgtagcg
     4261 ctcggtgagc acgtcgaggc acgggccgaa gttgacgtcc accccgaggc gccgcagctc
     4321 gcgcccctcg atgagcccct ggcgcgccac catcgcctcg tcgcccgcgg tgcccgcggt
     4381 gaggttgtcc gggaagatgg tgacgccccg gttgagcatg atgatgcggc cgccctcgtg
     4441 atcggtggtg atcaggagcc ggcgcccgag
//
LOCUS       DASUFA010000354         6453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_894750, whole genome shotgun sequence.
ACCESSION   DASUFA010000354 DASUFA010000000
VERSION     DASUFA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894750"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..438
                     /gene="hslV"
                     /locus_tag="VFX87_12960"
     CDS             1..438
                     /gene="hslV"
                     /locus_tag="VFX87_12960"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX4995884.1"
                     /translation="MKAGARKVRKMHHDRVLAGFAGAAADAFTLFARFEAKLEEYRGN
                     LPRAAVELAKEWRMDRVLRRLEALLAVADAEASFIVSGTGDVIEPDDGLVGIGSGGPY
                     ALAAARALRAHSALDAPQIAEESLRIAAGICVYTNDQITVLSL"
     gene            465..1814
                     /gene="hslU"
                     /locus_tag="VFX87_12965"
     CDS             465..1814
                     /gene="hslU"
                     /locus_tag="VFX87_12965"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX4995885.1"
                     /translation="MTGLTPAQITAELDRFIVGQGKAKRAVAIALRNRWRRQNLPAEL
                     RDEVAPKNIIMIGPTGVGKTEIARRLAKLAQAPFLKVEASKYTEVGYVGRDVESMIRD
                     LTELGVSMVKAEMAAAVRERAEALAEERLLDLLLPRRPGEAFVSGTLEEVSPDASRDA
                     TRDKLRAQLRSGKLDERPIEMDVQQAGPMLEVLPGQGMEEIGINLKDMLSNLMPGRTR
                     RRRVKVGEARRLLAQEEAQKLVDVDEAVGQAVRRVENSGIIFLDELDKIAGREGGRGP
                     DVSREGVQRDLLPIVEGSTVTTKYGMVRTDHILFIAAGAFHVAKPADLIPELQGRFPI
                     RVELEPLTRPDFIRILSEPQNALIRQYVELLRTEGVALRFSTDAVEAIAEIAFTVNQR
                     AENIGARRLYTVMERLLEEISFDAPNLTGQELVIDAEYVHAQLADVVKDEDLSRYIL"
     gene            1827..2702
                     /gene="argB"
                     /locus_tag="VFX87_12970"
     CDS             1827..2702
                     /gene="argB"
                     /locus_tag="VFX87_12970"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX4995886.1"
                     /translation="MTPIPELVQRAELLIEALPYIRAFQGKTLVIKYGGAAMEQADLK
                     EQFAKDVLLLRLVGIRPVIVHGGGPQIGALMKRLGKEPKFVGGMRVTDEETVEIVEMV
                     LVGKINKEIVGLINLHGGRAVGLSGKDASLLRAHKRLHKMPDGTQVDIGLVGEVEAVN
                     PEPIRLLEENGFIPVIAPVGVGAAGETYNINADLVAGDVAAALFAEKLIHLTDVTGIK
                     GEDGRLVSTLTKRDAERLIKAGVIDGGMLPKVESSLRALTGGAQKAHIIDGRVPHAIL
                     LEVLTKEGIGTEIVL"
     gene            2710..3912
                     /locus_tag="VFX87_12975"
     CDS             2710..3912
                     /locus_tag="VFX87_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4995887.1"
                     /translation="MTDTKTLIEWADKHLMTTAKRNPVVLVRGDGARVWDSDGKEYLD
                     FTGGIAVTALGHSHPKVVGTMREQAATLLHVSNLFYIPQQTQLAKLLCDHSFADRVFF
                     SNSGAEANESAIKLARKWAKEHGASDRGDIITMRGGFHGRTLATVTATAQEKYHHGFE
                     PLPGGFKYVPFNDLRAVERAIDSHTAAVMVEPIQGEGGVNIPDDGYLPGLRKLCDEAG
                     VLLVLDEIQTGMGRTGRLWGYEHSGIEPDIMTLAKALANGVPIGATLATESVASAFTP
                     GTHGSTFGGNPFVTAVGLTVFTTLMEERLPERAAATGRLLRDQLEAVRGRHPKVATAV
                     RGRGLLVGMDLVPPVGEVIGACRERGLLVLSAGDNTLRLAPPLITDEPTVRRAVEIID
                     RVLSGFVK"
     gene            3909..4826
                     /gene="argF"
                     /locus_tag="VFX87_12980"
     CDS             3909..4826
                     /gene="argF"
                     /locus_tag="VFX87_12980"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX4995888.1"
                     /translation="MSHFLSIRDVAREDLPRLFALMAELKARQKARDRRTPLPGRTMA
                     LIFEKPSLRTRVTFEVAMVQLGGAAVYLAAQEIGMGKRESVPDVGRNLSRWVDVVAAR
                     VFSHKTLEQLAANATIPVINGLSDLEHPCQAMADFFTLYERGLDPAKLRLAWVGDGSN
                     VCNSILLLGSMLGTTSVVACPPGYEPDAGVLEACRAMGGRVQITTEARDAVEEADVVY
                     TDTWISMGQEGDREQRLEAFQRYQVNDRLLDFAPGDALVMHCLPAHRGEEITDAVLDG
                     PRSLILDQAENRLHAQKAIIVNLLGGEQP"
     gene            4823..6031
                     /locus_tag="VFX87_12985"
     CDS             4823..6031
                     /locus_tag="VFX87_12985"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX4995889.1"
                     /translation="MTRSVKKVVLAYSGGLDTSVILRWLIETYRCEVVAYCADLGQGE
                     ELIPVKEKALRTGASSVHIVDLREEFVRDFVFPMLRANAVYEGSYLMGTSIARPLIAK
                     AQVEVARKERADAVSHGATGKGNDQVRFELTYAALGPDLTVIAPWREWDLDSRTALMD
                     FARRHDIPVPVTAERPYSTDRNLFHISFEGGILEDPWNEPPPKMFLLTNSPESAPDVP
                     VYVEIEYEAGNPVAVDGRRLSPAALLEAVNRLGGEHGIGRVDLVENRFVGMKSRGVYE
                     TPGGTILHAAHRALESITLDREVLHLRDSLVPRYAEMIYYGFWFSPEREAVQGLVDAV
                     QRDVTGTARLKLYKGTVTVAGRKSPRSLYRTDFVTFEADQVYRQRDAEGFINLNALRL
                     RIRALRDRAR"
     gene            6047..>6453
                     /locus_tag="VFX87_12990"
     CDS             6047..>6453
                     /locus_tag="VFX87_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX4995890.1"
                     /translation="MQVHVALTPAEFPDAPLTGCVALAVDVLRATTASVAACDAGCRR
                     LVPVPDAEAAEAMAARDGTGTILAGERGGEALAGFDLGNSPAEFTADRVGGRTVVLTT
                     TNGTAAMLTAARAAAAGRAALTNVTAAAGWALA"
BASE COUNT         1012 a         2196 c         2343 g          902 t
ORIGIN      
        1 atgaaggcgg gcgcgcgcaa ggtccgcaag atgcaccacg accgcgtgct cgccggcttc
       61 gcgggtgcgg ccgccgacgc cttcacgctc ttcgcgcgct tcgaggcgaa gctcgaggaa
      121 taccggggca acctgccgcg cgccgcggtc gagctggcca aggagtggcg catggaccgc
      181 gtgctccggc gactcgaggc gctgctcgcg gtagccgacg cggaagcgag cttcatcgtg
      241 tcgggcaccg gcgacgtgat cgagcccgac gacggcctgg tcgggatcgg ctcgggcggg
      301 ccctacgccc tggccgcggc gcgggcgctg cgggcgcact ccgcgctcga cgcgccgcag
      361 atcgcggagg agtccctccg catcgcggcc ggcatctgcg tgtacacgaa cgatcagatc
      421 accgtgctga gcctgtgacc gaccggcccg cccaccagat cgggatgacc ggcctcacgc
      481 cggcccagat caccgccgag ctcgaccgct tcatcgtggg tcaggggaag gccaagcgtg
      541 cggtggccat cgccctgcgc aaccggtggc ggcggcagaa cctgcccgcc gagctgcggg
      601 acgaggtggc gcccaagaac atcatcatga tcgggcccac tggagtcggg aagaccgaga
      661 tcgcccggcg cctcgccaag ctcgcccagg ctccgttcct caaggtggag gcctccaagt
      721 acaccgaggt cggctacgtg ggccgggacg tggagtccat gatccgcgac ctgaccgagc
      781 tcggcgtgag catggtcaag gcggagatgg cggcggcggt gcgcgagcgg gccgaggcgc
      841 tggccgagga gcggctcctc gatctcctgc tgccccgccg gcccggcgag gccttcgtga
      901 gcggcacgct cgaggaggtg tcgcccgacg cctcccgcga tgccacccgg gacaagctcc
      961 gggcccagct ccgctccggc aagctcgacg agcggcccat cgagatggac gtgcagcagg
     1021 ccgggcccat gctcgaggtc ctcccgggtc agggcatgga ggagatcggg atcaacctca
     1081 aggatatgct ctcgaacctc atgcccggcc gcacgcgccg acggcgggtc aaggtgggcg
     1141 aggcgcggcg gctgctggcc caggaggagg cccagaagct ggtggacgtg gacgaggcgg
     1201 tgggccaggc ggtccgccgg gtggagaact cggggatcat cttcctggac gagctggaca
     1261 agatcgcggg gcgggaaggg ggccgggggc ccgacgtctc ccgggaaggg gtgcagcgcg
     1321 atctcctgcc gatcgtcgaa ggctcgacgg tgaccacgaa gtacggcatg gtccggaccg
     1381 accacatcct gttcatcgcg gcgggggcct tccacgtggc caagccggcg gacctgatcc
     1441 cggagctgca ggggcgtttc cccatccggg tcgagctgga gcccttgacg cggccggatt
     1501 ttatccgcat tctgagcgaa cctcagaacg ccctgatccg gcagtacgtc gagctgctga
     1561 ggacggaagg agtggcgctg cgcttttcca ccgacgcggt ggaggccatc gccgagatcg
     1621 cgttcacggt gaaccagcgg gccgagaaca tcggggcccg ccgcttgtac acggtgatgg
     1681 agcggctgct ggaggagatc tccttcgacg ccccgaacct gacgggacag gagctcgtga
     1741 tcgacgcgga gtacgtgcac gcgcagctgg ccgacgtggt gaaggacgag gatctctccc
     1801 gctacatcct ataaggtgga cgaggcatga cgccgatccc ggagctggtg cagcgcgccg
     1861 agctgctcat cgaggcgctg ccctacatcc gtgccttcca gggcaagacc ctcgtgatca
     1921 agtacggggg cgccgcgatg gagcaggcgg acctcaagga gcagttcgcc aaagacgtgc
     1981 tcctcctccg gctggtcggc atccgtccgg tcatcgtcca cggcgggggc ccccagatcg
     2041 gcgcgctcat gaagcggctc ggcaaggagc cgaagttcgt gggcggcatg cgcgtcaccg
     2101 acgaggagac ggtggagatc gtcgagatgg tgctggtcgg caagatcaac aaggagatcg
     2161 tggggctgat caatctccac gggggccggg cggtggggct ttccggcaag gacgcgagcc
     2221 tgctccgggc ccacaagcgg ctgcacaaga tgcccgacgg cacccaggtg gacatcgggt
     2281 tggtgggcga ggtggaggcg gtcaatcccg agccgatccg gctgctcgag gagaacgggt
     2341 tcatcccggt gatcgcgccg gtcggagtgg gggcggcggg cgagacctac aacatcaacg
     2401 cggacctggt ggcgggcgac gtggccgccg cgctcttcgc ggagaagctg atccacctca
     2461 ccgacgtgac cggcatcaag ggcgaggacg gccgcctggt ctcgacgctg accaagcgcg
     2521 acgcggagcg gctgatcaag gcgggcgtga tcgacggggg catgctgccc aaggtggagt
     2581 cctctttgcg ggcgctcacc ggaggcgccc agaaggccca catcatcgac ggccgcgtgc
     2641 ctcacgcgat cctgctcgag gtgctgacca aggaaggtat cggcacggaa atcgtcctat
     2701 gagggagcca tgacggatac caaaacgctg atcgagtggg cggacaagca cctgatgacc
     2761 accgccaagc gcaacccggt ggtgctggtg cgcggcgacg gagcccgggt gtgggacagc
     2821 gacggcaagg agtacctcga cttcaccggc ggcatcgcgg tgaccgctct cgggcactcc
     2881 catcccaagg tggtggggac aatgcgagag caggcggcca cgctgctcca cgtgtcgaac
     2941 ctcttttaca tcccgcagca gacccagctc gccaagctgc tctgcgacca ctcgttcgcc
     3001 gaccgcgtct tcttctccaa ctcgggcgcc gaggccaacg agtccgcgat caagctcgcg
     3061 cggaagtggg ctaaggagca cggggccagc gaccgtggcg acatcatcac gatgcggggc
     3121 ggcttccacg gccggacgct ggccaccgtc accgccaccg cccaggagaa gtaccaccac
     3181 ggcttcgagc cgctgcccgg cggattcaag tacgtgccgt tcaacgatct gcgcgcggtc
     3241 gagcgggcca tcgacagcca caccgccgcg gtcatggtcg agccgatcca gggcgagggc
     3301 ggcgtcaaca tcccggacga cggctacctg cccgggctgc gcaagctctg cgacgaggcg
     3361 ggggttctgc tcgtgctgga cgagatccag accggcatgg ggcgcaccgg ccgcctctgg
     3421 ggctacgagc actccggcat cgagcccgac atcatgacgc tggccaaggc gctggccaac
     3481 ggggtgccca tcggggccac cctggccacc gagagcgtgg cgtccgcgtt cacgccgggc
     3541 acccacggct ccaccttcgg cggtaacccc ttcgtgaccg ccgtgggcct gaccgtgttc
     3601 acgacgctga tggaggagcg gctgcccgag cgcgcggccg ccaccggccg cctgctccgg
     3661 gatcagctcg aggcggtgcg gggccggcac ccgaaggtgg ccaccgcggt gcgcgggcgc
     3721 ggcctgctcg tgggcatgga tctggtgccg ccggtgggcg aggtcatcgg ggcctgccgc
     3781 gagcgcggcc tgctcgtgct cagcgcgggc gacaacacgt tgcggctggc gccgccgctg
     3841 attacggacg agcccaccgt gcgccgggcg gtcgagatca tcgaccgggt tttgagcggc
     3901 ttcgtgaagt gagccatttc ctgtcgatcc gcgacgtcgc ccgggaggat ctcccgcgcc
     3961 tcttcgccct catggccgag ctcaaggcgc ggcagaaggc ccgtgatcgc cggaccccgc
     4021 tgcccggacg caccatggcc ctgatcttcg agaagccctc gctgcgcacc cgcgtcacct
     4081 tcgaggtggc gatggtccag ctcggcggcg ccgcggtgta cctggccgcc caggagatcg
     4141 ggatgggcaa gcgcgagtcg gtccccgacg tgggccgcaa cctctcgcgc tgggtggacg
     4201 tggtggcggc gcgcgtgttc tcgcacaaga ccctcgagca gctcgcggcc aacgctacga
     4261 tcccggtgat caatggcctg tccgacctcg agcacccctg ccaggcgatg gcggacttct
     4321 tcaccctcta cgagcgcggg ctggatccgg cgaagctgcg actggcctgg gtgggcgacg
     4381 gcagcaacgt ctgcaactcg atcctgctgc tgggctccat gctggggacc acttcggtgg
     4441 tggcgtgccc cccgggctat gagccggacg cgggtgtgct ggaagcctgt cgcgcgatgg
     4501 gcggccgtgt gcagatcacc accgaggcgc gcgacgcggt ggaggaggcc gacgtggtct
     4561 acaccgacac ctggatcagc atgggtcagg aaggcgaccg cgagcagcgc ctggaggcct
     4621 tccagcgcta ccaggtcaac gaccggctgc tcgacttcgc gcccggcgac gcgctcgtga
     4681 tgcactgcct gcccgcgcac cgcggcgagg agatcaccga cgcggtgctc gacgggccgc
     4741 gcagcctgat cctggaccag gccgagaacc gtctgcacgc gcagaaggcc atcatcgtca
     4801 acctgctggg cggggagcag ccgtgaccag atccgtcaag aaggtcgtgc tcgcgtactc
     4861 cggaggcctc gacacctccg tcatcctccg ctggctcatc gagacgtacc gctgcgaggt
     4921 cgtggcctac tgcgccgacc tggggcaggg cgaggagctg atcccggtca aggagaaggc
     4981 cctgcgcacc ggcgcctcct ccgtccacat cgtggatctg cgggaagagt tcgtccgcga
     5041 cttcgtcttc ccgatgctcc gggccaacgc ggtctacgag ggctcgtacc tcatggggac
     5101 ctcgatcgcg cggcccctca tcgccaaggc ccaggtggag gtcgcgagga aggaaagggc
     5161 ggacgcggtg agccacgggg ccaccggcaa gggcaacgac caggtgcgct tcgagctgac
     5221 ctacgcggcc ctcgggcccg acctgaccgt gatcgcgccg tggcgggagt gggacctcga
     5281 ctcccgcacc gcgctcatgg acttcgcccg gcggcacgac atcccggtgc cggtgaccgc
     5341 ggagcgcccg tactccaccg accgcaacct cttccacatc tccttcgagg gcggcatcct
     5401 cgaggatccg tggaacgagc ctccgcccaa gatgttcctg ctcacgaact cgccggagtc
     5461 ggcgcccgac gtgccggtct acgtggagat cgagtacgag gcgggcaatc cggtggcggt
     5521 ggacggacgg cggctctcgc ccgccgcgct gctcgaggcg gtcaaccggc tgggcggcga
     5581 gcacggcatc gggcgggtgg acctggtcga gaaccgcttc gtgggcatga agtcgcgcgg
     5641 ggtctacgag acgcccggcg gcaccatcct gcacgcggcc caccgcgcgc tcgagtcgat
     5701 cacgctcgac cgcgaggtgc tgcacctgcg cgactcgctg gtgccgcggt acgcggagat
     5761 gatctactac gggttctggt tcagcccgga gcgggaggcg gtgcaggggc tcgtggacgc
     5821 cgtgcagcgc gacgtgaccg gcacggcgcg gctcaagctc tacaagggga ccgtgacggt
     5881 ggcggggcgc aagtcgccgc ggtcgctcta ccggaccgac ttcgtgacct tcgaggccga
     5941 tcaggtgtat cgccagcgcg atgcggaggg cttcatcaat ctcaacgcgc tccggctcag
     6001 gatccgcgct ctccgggacc gggcgcggta ggcggccgcg ctcccgatgc aggtccacgt
     6061 cgcgctgacc ccggcggaat tccccgacgc gcccctcacg gggtgcgtcg cgctcgcggt
     6121 ggacgtgctg cgggccacca ccgcgtcggt ggccgcctgc gacgcgggct gccggcgtct
     6181 ggtgccggtg ccggacgcgg aggctgccga ggccatggcc gcgcgcgacg ggaccggcac
     6241 catcctggcc ggcgagcggg gcggggaggc gctcgcgggc ttcgatctcg gcaactcgcc
     6301 cgccgagttc accgccgacc gggtgggcgg ccgcaccgtc gtgctgacca cgaccaacgg
     6361 caccgccgcg atgctgaccg cggcgcgggc ggcggcggcc gggcgcgccg cgctcaccaa
     6421 cgtgaccgcg gccgcgggct gggcgctcgc gca
//
LOCUS       DASUFA010000355         8607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1337343, whole genome shotgun sequence.
ACCESSION   DASUFA010000355 DASUFA010000000
VERSION     DASUFA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337343"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1745)
                     /locus_tag="VFX87_12995"
     CDS             complement(201..1745)
                     /locus_tag="VFX87_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995891.1"
                     /translation="MELTRRDLLKGAAALGAAATVPLTAGGAALAQTTQKKELVTAQA
                     GDISKFDPHFSTSSNDIRVSFNIFDNLTSRHPDGKLYPGLATEWKLEGQTTWRFKLRQ
                     GVKFHNGDAFTSADAKFSIERTYDPAAKTMVATVFSTIEKIEAPDPLTLVVTTKKPDP
                     LLPARLAFYGGQIVPKKYLESVGNDTFNAKPVGTGPVRFGSWVKDDKAVLEANPDYWG
                     GKIEVDRWTMRPIPETAPRIAALLKGEVDVITQLPPDQGERVTGNASTRVAGALYAGL
                     YVLAVNSKRPPLDNPLVKQALSLAIDRPAIVKELWRGRGIVPSGPIAKGDNHFDASLP
                     GMAYNPAEAKERLKKAGYKNEEIFIETTVAYVAQDKAMSEAIAAMWKDVGVNVKVEVI
                     EYSVRAQKNREKSFKGVFWSDPTSTLGDPDGMMWRLLGPGGPQDYWREAKFDELGNAA
                     RFSVDEKFRAGAYKEMTRIFLENLPWLPVIQPYEDYGLQKYVDFTPNPNQQFEVRRFN
                     LKLRRA"
     gene            1883..3070
                     /locus_tag="VFX87_13000"
     CDS             1883..3070
                     /locus_tag="VFX87_13000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4995892.1"
                     /translation="MSATADIVIIGGGANGTSTAFHLASMGAKNVRLLERRHLAAGAT
                     GKSGALVRMHYTNEHESRLALESLRIFRNFASIVGGDCGFEAPGFLQLVPPGYEAALR
                     RNLARQQRLGVDTREVSRDDVRELFPECRVDDIGAAAWEPGSGYADPSATAFAFAAAA
                     QRLGVTIENGCHVTRILTERGRVTGVETARGRVEAPVVVLVPGAWGQPLLDPLGLDYG
                     LQPFRIQVSLFRWPPTLTRKHPVVIDAGQKAWIRPVDGNLSLIGVELGAAYGGDPDKL
                     HEGVDEGYVALCRERLSHRLPLFADSTMRGGWAGMIMMSPDGRPIIDQIPTVPGLYVM
                     LGDSGTSFKTSPAIGRCLAEWILEGKPRTVDLTPFRAARFAEGRPWVDDDNYGRERLT
                     ISR"
     gene            complement(3156..5492)
                     /locus_tag="VFX87_13005"
     CDS             complement(3156..5492)
                     /locus_tag="VFX87_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4995893.1"
                     /translation="MAWWDLPSKLLVAASLAVLLGLLAGLAMDAGRPYPGFFATPDYH
                     VVPVEPVARAAGLRVGDRLIAVDGGSPLTLAARMRGTAAPIRYDVERAGRRFVVQLAP
                     GPLTWSRLVDHFAVYFLVSALMLVVGAAVYVQNPAAAPNRRFLLYMCLWAVANVAVPE
                     AVLGARPYAAALVGLLAPLLSIHGWVFFLTYPANAERERWLERHRVIPRLYRVAVVLG
                     LASALSFILIRAFAPGLLLDGWVYPASAAVLSVLSTLSFPIKVGALLDTRRRAASPLV
                     NQQTTVLLLGIAMGLGAWLCLMLWPLQQLYESPLDPQVGSALMLLYPAAIGYATVRYR
                     LFDATVVIRRSVIYTGLAGLITGAYALLLAGTNALLAQADLTRSPWFSAGFMFVVVLL
                     FNPLRERVRRAVDRTFFRERHDYALAMRTLARSMSSLLDLDEITRRLTTTIESSMHVR
                     AARLLVDGFPHDVLAALEVAPGALSRYQLAADPRFGSGRGAALAAYQALGAEVVVPLR
                     FQARLRALLVLGGKRSEAPYTGEDLEMLEALADQAAVAVANAEAHRQVLDYAQQLERS
                     LLIRGSLAKFVPRRVRQLIEESPEAPSLEKRETDVSVLFADISGYTRLSSRLAPDDLA
                     GLVERYFGAFLDEIVEHGGDVNETAGDGLMVIFHEGPHARAAVDAARAIHRRAAEIGA
                     QLAARFEPVAMHVGVNTGPALLGATKIEGSAGTRWTYTASGMTTNIAARLAAQAGDGE
                     IVISEATRTRLGDDLPMEDLGVRGLKNVDTPMRLFRLR"
     gene            5517..7616
                     /locus_tag="VFX87_13010"
     CDS             5517..7616
                     /locus_tag="VFX87_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX4995894.1"
                     /translation="MTDLSAVFRPRAIAVVGASDDPVKIGGRPLAFLLRHGYAGRVFP
                     VNPARATVQGLPAFPSIAAIPEEVDLAIVVVPAERVLESLEEAAAKGVRAAIVFSSGF
                     AEVGEAGRVAQARLRELAARTGLRIIGPNCQGFAHLPSRLVATFASPFLEPGLATGPI
                     AMVSQSGAMAGMIYEMARAAGLGLNYWVSTGNEADVQAAEILAEVVEDEETRVACCYL
                     EDIKDAGRFRRALARAHRRGVPVFVLKSGRSAVGRRAASSHTGALAGEDAVYDAVFAD
                     WGAIRCADPAELLALPQAFLRYREAGRRVAILSNSGGLGVLSVDLCVDLGLVPAEFTP
                     ETTAVLRGALPAFAAAANPVDLTAQMLTDPGMLMRVLPALEADPGVDAIVFQIALLGA
                     ATDLGRLVGDVAAVARSSRKVVAVSCPQRPVVEAFRGGGVLAFDDASVALRSLACLAE
                     ATARRPRWLDRATLEAPPPPPAPVVPAGRPGEPFLNEWESQRLLTPFGLPLVDTVFVT
                     AATAAPDAAEQLGYPVVVKACAAALPHKSDVGGVALGLSDRAAVAEACRRIEANVRGA
                     VPGLALDGFLVQRQARGALELALGVKVDPVFGPVVLVGSGGVLIEVLRDFRLLLPPID
                     APAAEEALRNLRIGPLWDGVRGGAALDLPAAVELLQRLGDAARSLGAVISEIDLNPVL
                     VGRRGEGVTVLDALVRR"
     gene            complement(7636..8043)
                     /locus_tag="VFX87_13015"
     CDS             complement(7636..8043)
                     /locus_tag="VFX87_13015"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX4995895.1"
                     /translation="MIELTLEMAERATRAAREKAKALGTPMTITVVDEAGRLVLCSRG
                     DGTGFFTPETSRAKAMAAANFRMSTADLADRVQKGSPFWAQVGSAVPGQLLASHGAVP
                     IVVKGRVIGAIGCGGGTGEQDQECAAAGAAVLA"
     gene            8385..>8607
                     /locus_tag="VFX87_13020"
     CDS             8385..>8607
                     /locus_tag="VFX87_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995896.1"
                     /translation="MRQAALLLAVSALAMGGCGSLSGGLHSESMTAATAGATDPDNPV
                     AAALAPEQQPTGAVVEPRAGEQAMAATVAF"
BASE COUNT         1170 a         3107 c         3101 g         1229 t
ORIGIN      
        1 cgcgtcggga ggcagcagga gcggcaccgg gtcgccggac aggcgcatga ccacgaagac
       61 cacggtggac acgagccaga gcgcgatgag ggcgcggaag aggcggtgac cgagaaacgg
      121 gacaaatccg cgcatggctg aactcccgca cctccaccct ctcccccacg agggggagag
      181 ggcggggcga gggggaacga ctacgcgcga cgaagcttca ggttgaagcg gcgcacctcg
      241 aactgctggt tcgggttcgg cgtgaagtcc acgtacttct ggaggccgta gtcctcgtac
      301 ggctggatga cgggcagcca gggcaggttc tccaggaaga tcctggtcat ctccttgtag
      361 gccccggccc ggaacttctc gtccacgctg aagcgggccg cgttgcccag ctcgtcgaac
      421 ttggcctcgc gccagtagtc ttgcgggccg cccgggccga gcaggcgcca catcatgccg
      481 tccggatccc cgagagtgga cgtcgggtcc gaccagaaca cgcccttgaa gctcttctcg
      541 cggttcttct gggcgcgcac cgagtactcg atgacctcga ccttgacgtt gacgcccacg
      601 tccttccaca tggccgcaat ggcttccgac atcgccttgt cctgcgccac gtaggccacc
      661 gtggtctcga tgaagatctc ctcgttcttg tagcccgcct tcttgagccg ctccttcgcc
      721 tccgccgggt tgtaggccat ccccggcagg ctcgcgtcga agtggttgtc gcccttggcg
      781 atgggcccgc tcggcacgat gccgcggcca cgccacagct ccttcacgat ggccgggcgg
      841 tcgatggcca gcgacagcgc ctgcttcacg aggggattgt cgagcggcgg ccgcttgctg
      901 ttcaccgcga gcacgtagag gcccgcgtag agcgcccccg ccacccgcgt ggaggcgttg
      961 ccggtcaccc gctcgccctg gtcgggcggg agctgggtga tgacgtcgac ctcgcccttg
     1021 agcagcgcgg cgatgcgggg cgccgtctcc gggatcggcc gcatggtcca gcggtccacc
     1081 tcgatcttgc cgccccagta gtcgggattg gcctcgagca ccgccttgtc gtccttcacc
     1141 caggagccga agcgcaccgg gcccgtgccg acgggcttgg cattgaaggt gtcgttgcct
     1201 accgactcga gatacttctt cggcacgatc tggccgccgt agaaggccag ccgcgcgggc
     1261 agcagcgggt ccggcttctt cgtggtgacg acgagcgtca ggggatccgg cgcctcgatc
     1321 ttctcgatcg tggagaacac ggtggccacc atcgtcttcg cggccgggtc gtaggtgcgc
     1381 tcgatgctga acttggcgtc ggcggaggtg aacgcgtcgc cgttgtggaa cttgacgccc
     1441 tggcgcagct tgaagcgcca cgtggtctga ccctcgagct tccactcggt ggcgagcccg
     1501 gggtagagct tgccgtccgg atggcggctc gtcaggttgt cgaagatgtt gaaggacacc
     1561 cggatgtcgt tcgacgacgt gctgaagtgc gggtcgaact tggagatgtc gcccgcctgc
     1621 gcggtcacca gctccttctt ctgggtggtc tgggccagcg cggctccgcc cgcggtcagc
     1681 ggcaccgtgg cggcggcgcc gagggcggcc gctcccttga gcagatcacg tcgggtcaat
     1741 tccatggttg gtgctcctcc tcgattcgaa cggcgtcggt tgaaagctgt ttggggtccg
     1801 tcatttatag gggtttcccc tcaccctgtc aagccgggcc gccggtggcg aacccgtgcc
     1861 aaacatgaca gaatccaacg ccatgagcgc gacggcggac atcgtcatca tcggcggcgg
     1921 agcgaacggg acgagcaccg cgtttcacct ggcgagcatg ggcgcgaaga acgtccggct
     1981 gctggagcgc cgccacctgg cggccggcgc caccggcaag tccggggccc tcgtgcgcat
     2041 gcactacacc aacgagcacg aatcgcgcct cgccctcgag agcctccgga tcttccgcaa
     2101 cttcgcgtcc atcgtcggcg gcgactgcgg cttcgaggcc ccggggttcc tccagctcgt
     2161 gccgcccggc tacgaggcgg cgctgcgccg caacctggct cgtcaacagc gactcggcgt
     2221 agacactcga gaagtctcgc gcgacgacgt ccgcgagctc ttccccgagt gccgcgtgga
     2281 cgacatcggc gccgccgcgt gggagcccgg ctccggctac gccgatccga gcgccaccgc
     2341 gttcgccttc gcggccgccg cgcagcgcct gggcgtcacc atcgagaacg gctgccacgt
     2401 cacccggatc ctgaccgagc gcggccgcgt caccggcgtc gagaccgcgc gcgggcgcgt
     2461 ggaggccccg gtggtcgtgc tggtgcccgg cgcctggggc cagccgctgc tcgatccgct
     2521 cggcctcgac tatgggctgc agcccttccg catccaggtc tcgctcttcc gctggccgcc
     2581 gacgctgacc cgcaagcacc cggtggtgat cgacgcgggc cagaaggcct ggatccgccc
     2641 ggtggacggc aacctgagcc tgatcggcgt cgagctgggc gcggcctatg gcggcgaccc
     2701 ggacaagctg cacgagggcg tggacgaggg ctacgtggcg ctctgccgcg agcggctcag
     2761 ccaccgcctg ccgctcttcg ccgactccac gatgcgcggc ggctgggccg gcatgatcat
     2821 gatgagcccc gacggccgtc cgatcatcga ccagatcccg acggtgcccg gcctctacgt
     2881 gatgctgggc gactccggca cgtccttcaa gacctccccc gccatcgggc gatgcctggc
     2941 cgagtggatc ctcgagggca agccgcgcac cgtggatctg actccgtttc gcgccgcccg
     3001 cttcgccgag ggccgcccct gggtcgacga cgacaactac ggccgcgagc gcctgaccat
     3061 ctcgcgctag ttctccccct ctcccctctg gggagagggc agggtgaggg gctctgcagg
     3121 gtgaggggct ctggcggtga gagacggcga gccccctacc gcagccggaa cagccgcatc
     3181 ggcgtgtcca cgttcttcag cccgcgcacg cccaggtcct ccatcggcag gtcgtccccc
     3241 agccgcgtcc gcgtcgcctc gctgatgacg atctcgccgt cgcccgcctg cgcggccagg
     3301 cgcgccgcga tgttggtggt catgccggag gcggtgtagg tccagcgggt acccgcgctg
     3361 ccctcgatct tggtcgcgcc cagcagcgcc gggccggtgt tgacgcccac gtgcatggcc
     3421 accggctcga agcgcgcggc cagctgggcg ccgatctcgg cggcccggcg gtggatggcc
     3481 cgcgccgcgt ccaccgccgc gcgggcgtgc gggccctcgt ggaagatcac catgagcccg
     3541 tcgcccgcgg tctcgttcac gtcgccgccg tgctcgacga tctcgtccag gaaggcgccg
     3601 aagtagcgct ccaccaggcc tgccaggtcg tcgggcgcaa ggcgcgagga cagacgcgtg
     3661 tagccgctga tatcggcgaa cagcacgctg acgtcggtct cgcgcttctc cagcgacggc
     3721 gcctccggcg attcctcgat gagctggcgg acccggcgcg gcacgaactt ggcgaggctg
     3781 ccgcggatga gcaggctgcg ctcgagctgc tgggcgtagt cgagcacctg gcggtgcgcc
     3841 tccgcgttcg ccaccgccac cgcagcctgg tcggccagcg cctccagcat ctcgaggtcc
     3901 tcgccggtat agggcgcctc cgaccgcttg ccgcccagca ccagcagcgc gcgcagccgc
     3961 gcctggaagc gcaggggcac caccacctcc gcgcccagcg cctgataggc ggccagcgcc
     4021 gccccgcggc cgctgccgaa gcgcggatcg gcggcgagct ggtagcgcga gagcgcgccc
     4081 ggggcgactt cgagcgcggc caggacgtcg tgcgggaagc cgtccacgag caggcgcgcg
     4141 gcgcgcacgt gcatggacga ctcgatcgtg gtggtcagcc gccgcgtgat ctcgtccaga
     4201 tcgagcaggc tcgacatcga gcgggccagc gtgcgcatgg ccagcgcgta gtcgtggcgc
     4261 tcgcggaaga acgtgcggtc caccgcccgc cgcacccgct cgcgcagcgg gttgaagagc
     4321 agcacgacca cgaacatgaa tccggccgag aaccagggcg agcgcgtgag gtcggcctgg
     4381 gccagcagcg cattggtccc ggccagcagg agcgcgtagg cgccggtgat gagcccggcc
     4441 agcccggtgt agatcaccga gcggcggatc accacggtgg cgtcgaagag gcggtagcgc
     4501 acggtggcgt agccgatggc ggccgggtag agcaacatga gggcggagcc cacctgcgga
     4561 tcgagcgggc tctcatagag ctgctggagc ggccagagca tgaggcagag ccaggcgccc
     4621 agccccatcg cgatgcccag cagcagcacg gtcgtctgct ggttgacgag gggcgaggcg
     4681 gcccggcggc gcgtgtcgag cagcgcgccc accttgatcg ggaacgacag cgtgctgagc
     4741 accgagagca ccgccgcgga ggccgggtag acccagccgt ccagcaggag ccccggcgcg
     4801 aaggcccgga tcaggatgaa cgagagcgcg ctcgccagcc cgagcaccac cgccacgcgg
     4861 tagaggcgcg ggatcacgcg gtggcgctcg agccaccgct cgcgctcggc attggccgga
     4921 tacgtgagga agaagaccca gccgtggatg gagagcagcg gggccaggag cccgaccagg
     4981 gccgccgcgt acgggcgcgc gcccagcacc gcctcgggca ccgccacgtt ggcgacggcc
     5041 cagaggcaca tatagaggag aaagcggcgg ttgggtgccg cggcggggtt ctggacgtag
     5101 accgcggcgc ccacgaccag catgagggcc gagaccagga agtagaccgc gaaatggtcc
     5161 accagccggc tccaggtcaa gggccccggc gccagctgca ccacgaaccg gcgcccggcg
     5221 cgctccacgt cgtaccggat gggcgcggcg gtcccccgca tccgggcggc cagggtgagc
     5281 ggcgaaccgc cgtccaccgc gatcagccgg tcgcccacgc gcaggcccgc cgcgcgcgcc
     5341 acgggctcga caggcacgac gtggtagtcg ggcgtggcga agaagcccgg ataggggcgg
     5401 cccgcgtcca tggccaggcc ggccagcaac cccagcagca ccgcgaggct cgccgcaacg
     5461 aggagcttcg atggcaggtc ccaccacgcc atacgggtac tctatactgc ggttccgtga
     5521 ctgacctcag cgcggtcttc cggcctcggg ccatcgcggt cgtgggcgcc tccgacgatc
     5581 ccgtcaagat cgggggccgg ccgctcgcct tcctgctgcg ccacggctac gcggggcgcg
     5641 tgttcccggt caatcccgcg cgcgccaccg tgcagggcct gcccgccttc ccctcgatcg
     5701 ccgcgatccc ggaggaggtg gacctggcca tcgtggtggt gcccgccgag cgcgtgctcg
     5761 agtcgctcga ggaggccgcg gccaagggcg tgcgcgcggc catcgtattc agcagcggct
     5821 tcgcggaggt cggggaggcc gggcgcgtcg cgcaggcgcg cctgcgggag ctggccgcgc
     5881 gcaccgggct gcgcatcatc gggcccaact gccagggctt cgcccacctg ccctcgcggc
     5941 tcgtcgccac cttcgcctcg ccgttcctcg agcccgggct cgccaccggg cccatcgcga
     6001 tggtgagcca gagcggcgcc atggccggga tgatctacga gatggcccgc gcggcggggc
     6061 tcggcctcaa ctactgggtc agcaccggca acgaggccga tgtgcaggcg gccgagatcc
     6121 tggccgaggt ggtggaggac gaggagacgc gcgtggcctg ctgctacctc gaggacatca
     6181 aggacgcggg gcgcttccgc cgggcgctgg cgcgggcgca tcgccggggc gtgccggtct
     6241 tcgtgctgaa gtcgggccgc agcgcggtcg gccgccgggc ggcctcctcg cacaccggcg
     6301 cgctcgcggg cgaggacgcg gtctacgacg cggtcttcgc cgactggggc gcgatccgct
     6361 gcgccgaccc ggccgagctg ctggcgctgc cgcaggcctt cctgcgctac cgggaggccg
     6421 ggcgccgcgt ggccatcctc tccaactcgg gcggcctcgg cgtgctctcc gtggatctct
     6481 gcgtggatct ggggctcgtc cccgcggagt tcacaccgga gacgaccgcg gtgctgcggg
     6541 gggcgctgcc ggccttcgcg gccgccgcca atcccgtgga cctcaccgcg cagatgctca
     6601 ccgaccccgg catgctcatg cgcgtgctgc ccgcgctcga ggccgacccc ggcgtggacg
     6661 ccatcgtgtt ccagatcgcc ctgctcggcg ccgcgaccga tctcggccgg ctggtcggcg
     6721 acgtcgccgc ggtggcccgg tccagccgca aggtcgtcgc agtgagctgc ccgcagcgcc
     6781 ccgtggtcga ggccttccgg gggggcggcg tgctcgcctt cgacgacgcc agcgtggcgc
     6841 tccgctcgct ggcctgcctg gccgaggcga cggcgcgccg cccgcgctgg ctcgatcgcg
     6901 cgacgctcga ggcgcccccg ccgcctccgg cccccgtggt accggccggc cggcccggcg
     6961 agccgttcct caacgagtgg gagagtcagc ggctcctcac gcccttcggg ctgccgctgg
     7021 tcgacaccgt cttcgtcacc gccgccacgg ccgcgcccga cgcggccgag cagctcgggt
     7081 acccggtggt ggtgaaggcc tgcgcggcgg ccctccccca caagtcggac gtgggcggcg
     7141 tggccctcgg gctctcggac cgcgccgcgg tcgcggaggc gtgccggcgc atcgaggcca
     7201 acgtgcgcgg cgcggtgccg ggccttgccc tcgacggctt cctggtgcag cgccaggccc
     7261 gcggcgcgct cgagctggcg ctcggcgtga aggtggatcc cgtcttcggc ccggtcgtgc
     7321 tggtgggctc gggcggggtg ctcatcgagg tcctgcgcga tttccggctg ctgctcccgc
     7381 cgatcgacgc gcccgccgcc gaggaggcgc tccgcaacct gcgcatcggc ccgctgtggg
     7441 acggcgtgcg cggcggcgcg gcgctcgatc tacccgccgc cgtcgagctg ctgcagcgcc
     7501 tgggcgacgc cgcgcgcagt ctcggcgccg tgatcagcga gatcgacctg aacccggtcc
     7561 tggtgggccg gcgcggcgag ggcgtgaccg tcctcgacgc cctcgtgcgc cgctagcggg
     7621 gcaggccgcg cggcgtcagg cgaggaccgc ggcgcccgcg gccgcgcact cctgatcctg
     7681 ctcgccggtg ccgccgccgc agccgatggc gccgatcacg cggcccttca ccacgatcgg
     7741 caccgcgccg tggctggcca gaagctggcc cgggaccgcg ctccccacct gggcccagaa
     7801 cggcgagccc ttctgcacgc ggtcggccag atcggcggtg gacatgcgga agttcgcggc
     7861 ggccatcgcc ttggctcgcg aggtctcggg cgtgaagaag ccggtgccgt cgccgcgcga
     7921 gcacagcacc agccgtcccg cctcgtccac caccgtgatc gtcatcgggg tgccgagggc
     7981 cttggctttc tcgcgcgccg cgcgtgtcgc tcgctcggcc atctccagcg tgagctcgat
     8041 catttgccgg ccacctttct cgaagcacct tccgggatcc tgcgatcgcg cccgcattga
     8101 agcccggcgc ctgcgcgctg tcaacggccc ccggagtgac aaatccgggg agcgcgctac
     8161 ccagtcctgc ccgggatgga ctataggtgg gggccgcgcc gacgcggtgt cgcagggaaa
     8221 gtgcttgaaa gaatgacgct cggtatccta tgctcgcgtg tggcgcgccg tggcactggc
     8281 cttgctccgt tggtgccggg ttcgccggcc tcggccagcg agcccaaatg agcgacgcca
     8341 gccagaactc gagggggaca cggaccgtgc gtttcggatt cgggatgcga caggcagcgc
     8401 tgctgttggc ggtgtcggcc cttgccatgg gcgggtgtgg ctcgctgtcg ggtgggctac
     8461 acagcgagtc catgacggcc gcgaccgcgg gagcgaccga tccggacaat cccgtcgccg
     8521 cggcgctcgc ccccgagcag cagcccaccg gcgcggtggt cgagccccgg gcgggcgagc
     8581 aggccatggc cgccacggtg gcgttcc
//
LOCUS       DASUFA010000356         9044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_90549, whole genome shotgun sequence.
ACCESSION   DASUFA010000356 DASUFA010000000
VERSION     DASUFA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90549"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFX87_13025"
     CDS             complement(<1..703)
                     /locus_tag="VFX87_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX4995897.1"
                     /translation="MARPKRGETLSLTIDDLAFGGEGVGRADGYVVFVPGGLPGDRLR
                     VRLVQVRSRFGRGVIDAIEEPSPQRVEAPCPYFGRCGGCRLQHMAYPAQLAFKAKQVV
                     DALERLGGLKGIEVRPIIGATETFGYRNKMEFTVARGSKDPMVVGLHEADRYDSVLDI
                     DRCLLQSDRMNALLGEARDFFIERGLTVYEQDSGEGLLRFLMLREGTHTGELMANVVT
                     SAPAVSELAPLAQRLQ"
     gene            841..3036
                     /locus_tag="VFX87_13030"
     CDS             841..3036
                     /locus_tag="VFX87_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX4995898.1"
                     /translation="MLTVALPALEERLLAERIRQGWRGAVGAELAHRTRPGELRAGTL
                     TVMVDNSPWLQELSMRSAEVLEAVRGRFGPTVTALRLSLAGPAPAPERPTPRRAAPAA
                     PLRLDADELALVEDAAAPVRDEALASTVRRIMAKGLIARRGGALLGLLLAVAALAGCV
                     STQTSPGEPAGPKTITIVPPGRRAVSAQGEAYDHYAIAQMEARAGRMPQAIAQLREAI
                     KLDPNTAALWIQLSQWLIRSNDTAGAFTAAQKAVALEPNNAGAHMTMADLYKRQRKPA
                     EAEAELERAVALDPQTPDGYLALAQLHFEGKSYDKARAVLRRLVTARPNLSQGYYLLG
                     RIGIENEQWDEALTNLKRAVELDPDHDGAWSALGFVYEAQNKLDDAVAVYKDALKANP
                     DNAAFVERLGDLLVKLGRYGEAQSEIESLVDSLPRDPRVWLKLGAIHYEQKQYDKAVA
                     AFRQAVALEPGNMRTRYYLATALMDSGKDDEALSELEKILKADPRSVDARVQLGFLYG
                     RAKRYDEAVGTLQEAVNLEPKRPELFLYLGTALQRASQYDRSASVLREGLSVDDKNKD
                     LHFQLGVVLEKQHQFDDAIQSFRRVLVLDPKHAESYNYIGYMYAEKGTNLSEAIQLIQ
                     QALALEPQNGYFIDSLGWAYYQQGRYPEALRELKRAVSLAKEDPVLYEHLGDAYLKNN
                     LANEAIEAWEKSLQVDPNAPTAPQVRNKIRDARENQLRVKGAPPKAEQK"
     gene            3008..3802
                     /locus_tag="VFX87_13035"
     CDS             3008..3802
                     /locus_tag="VFX87_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995899.1"
                     /translation="MRRPRPSRSSGLRLAGGLLLAASLSACATAPPAPAPPPIAEEAR
                     AALARIEQHRRSLVDLRSLADITVRRGGRAQRLSGVMLLLRETSSLRFEALSPFGMPV
                     LIVAGDPQSLTLWEVLDNRAYLAPASPEANRRWLGLALGGEDLVALLAARARPLPDPT
                     RVEMVGPDETGPSVRLTGRDVEQRIWFDPASGQARQVEWTGKNPARVTFTPTPPEAPP
                     AGLRLATPDGTLRVSVTYRDPKMNTGLDPELLKLTVPEHVRIQDFR"
     gene            3870..4733
                     /locus_tag="VFX87_13040"
     CDS             3870..4733
                     /locus_tag="VFX87_13040"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX4995900.1"
                     /translation="MLRTSAKVNLALEVLGKRGDGYHEIATVLQAVDLFDRLKLEAAD
                     TLSLHTDDPDLPTDDGNLVMRAARLLQKAAGIEAGARIRLTKRIPVAAGLGGGSSDAA
                     ATLLGLHRLWRLRWPRARLQELAVELGMDVPFFLGSGRAVARGRGEQLTALPGGGGYA
                     LVLVNPRVPLSTREVYGRVPAGWRAEPTGTERVIEALRTRNVSKMAAALTNNLESVVE
                     PVLPVIGRMKAALLAAGALGAIMSGSGPTVFGMARSLDHARQIRTRVSRAEWACWAVR
                     TNSGPAIRVSR"
     gene            4752..4825
                     /locus_tag="VFX87_13045"
     tRNA            4752..4825
                     /locus_tag="VFX87_13045"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4784..4786,aa:Gln,seq:ttg)
     gene            4954..5895
                     /locus_tag="VFX87_13050"
     CDS             4954..5895
                     /locus_tag="VFX87_13050"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX4995901.1"
                     /translation="MAYELKLFSGNANRPLAEEIAKTLQLPLGDADVSRFSDGEVYVQ
                     INENVRGQDVFVIQPTCPPVNDHLMELLVMIDAFKRASARRITAVLPYYGYGRQDRKV
                     QPRVPITAKLVADLLTAAGAQRLLAVDLHAGQIQGFFNIPVDHLFAAPPVIIDHLAKK
                     DLNDPVLVSPDAGGVERARALAKRLKAGLAIIDKRREGNNVAQFMHLIGDVKGRDAVI
                     VDDMIDTAGTMIQAVDALRREGANRVLACAVHGVLSGPAINRIKESILEEVIITNTVP
                     LTPERQLSKIRVLSVAPLLAEAIRRIHDEESVSTLFV"
     gene            5925..6623
                     /locus_tag="VFX87_13055"
     CDS             5925..6623
                     /locus_tag="VFX87_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX4995902.1"
                     /translation="MEMRELTIRKREGIGKGPARRLRRAGEVPATLYGGASPVNVAVM
                     PKDVLRLIHGREGSTQLFRVTFEGTTDGKMAIIRDMQFDPVSENLLHVDLQEVAMDRA
                     IQVTVAVHHVGEAIGVKDTQGILEMVLREVRVSCLPGNIPEHLEADITNLAIHDVLTV
                     KDLKVPEGVRVLNDPNQAVVTVAPPAAEEAVAAAAVPTEAAAAEPEVLTERKPKEGEA
                     AEGDAKDKGGEKKK"
     gene            6624..7166
                     /gene="pth"
                     /locus_tag="VFX87_13060"
     CDS             6624..7166
                     /gene="pth"
                     /locus_tag="VFX87_13060"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX4995903.1"
                     /translation="MAQAIVGLGNPGPEYRNTRHNVGQRVVDALAHRIHARFERDSGH
                     AVAPGRWHGEPLYLIKPGSFMNVTGAPVARLARKLHLGPADLIIVFDDLDLPLGKVRV
                     RLTGSAGGHNGVRSLIAAFGTNDLRRVKIGIGRPGRPGLDRDRVSDHVLSPFFPEEQE
                     AIAAACDEAADEALALVERR"
     gene            7180..>9044
                     /locus_tag="VFX87_13065"
     CDS             7180..>9044
                     /locus_tag="VFX87_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX4995904.1"
                     /translation="MSPAFRLSAGSGGLEWPQVLALLAREARTPMGRELATAAVPFTD
                     PGAIRRALGETRQARAAMGQTGVPPLEGIPDVRPTLETARVAGSVAEAADLAALIPLL
                     EAAGRLLAYGRAIQPVAPALAEALAGFPSQTELADLLRRSLDADGQVRDEAAPALRRV
                     RQRIRDLRRDLVKRLEAYFAAPSADTTFQERYVTVRHGRYVLPIRAEAKGRLRGIVHD
                     RSQSGATLFVEPEALVEANNDLVQATREEETEILRVLAALTDAVRAALPELDDLVAGI
                     GGLDLIFARGALAERMEAVEPTIADERDVLLRGALNPLLLAQSWATEGGRPVIPMDLE
                     IGSERPVLVITGPNAGGKTVALKTLGLLVLMAQAGCHVPARDGARLPVFSQCFAIVGD
                     EQSVAENLSTFSAFVKQLREVLARVDARSLVLLDELGAGTDPDDGAALAQAVLEDLAE
                     RGAVVVASTHLEPLKGFASTHPRARNASVEFDPERLAPTFRLIYDRPGQSYALSIGAR
                     LGLPAALIARAHAHRSTQQRQLQELLARLDDRDRKDAERRAQLERREAESAGLLARAQ
                     AELEAARASARETIARAKAEAQRLVTEVRRHVNEEWDRLKRAEKSRPELERARKRL"
BASE COUNT         1388 a         3263 c         3187 g         1206 t
ORIGIN      
        1 cctgcaggcg ctgggcgagc ggggccagct cggagaccgc cggcgcggac gtgaccacgt
       61 tggccatgag ctcgccggtg tgcgtgccct cgcgcagcat gaggaagcgc aggagcccct
      121 cgccgctgtc ctgctcgtag acggtgagcc cgcgctcgat gaagaagtcg cgcgcctcgc
      181 cgaggagcgc gttcatgcgg tccgactgca gcaggcagcg atcgatgtcg agcaccgagt
      241 cgtagcgatc ggcctcgtgc agcccgacca ccatcggatc cttcgagccc cgcgccaccg
      301 tgaactccat cttgttccgg tacccgaagg tctcggtcgc gccgatgatc ggccggacct
      361 cgataccctt gaggccgccg agccgctcga gcgcatccac cacctgcttg gccttgaagg
      421 cgagctgggc gggataggcc atgtgctgga ggcggcagcc gccgcagcgc ccgaagtagg
      481 ggcagggcgc ctccacgcgc tgcggcgaag gctcctcgat cgcgtcgatg accccgcgcc
      541 cgaagcggga gcgcacttgc acgagccgca cacggagacg atcgccgggc aggccgcccg
      601 ggacgaagac cacgtagcca tcggcccgcc cgacgccctc gccgccgaag gccaggtcgt
      661 cgatcgtcag ggacagagtc tccccgcgtt tgggtcgggc catggtagcg acactataaa
      721 tgtcggtacg atcggagtca atttggcccc gcgttcaagg tggcgagtac atacccggtc
      781 aagtatcatg gatccacgat gcgctcgggg cgcttctcct ttccggtccg cgtaggcacg
      841 atgctgaccg tcgccctgcc cgctctcgaa gagcgcctgc tggcagagcg catccgccag
      901 ggctggcgtg gggccgtcgg cgccgagctg gcccatcgca cgcgtcccgg cgagctgcgg
      961 gccggcacgc tcacggtgat ggtcgacaat tccccctggc tccaggagct ctccatgcga
     1021 tccgcggaag tcctcgaggc ggtccgcggc cggttcggcc cgaccgtcac cgcgctgcgc
     1081 ctcagcctcg ccggccccgc gccggctccg gagcgcccca ccccgcgccg cgcggcgccc
     1141 gcggccccgc tgcggctcga cgccgacgag ctggccctcg tggaagacgc cgccgcgccg
     1201 gtgcgcgacg aggcgctcgc ctccaccgtc cgccgcatca tggccaaggg cctgatcgcg
     1261 cgccggggcg gggccctcct ggggctcctg ctcgcggtgg ctgcgctcgc cggctgcgtc
     1321 tccacccaga cgagcccggg cgagcccgcg gggcccaaga cgatcacgat cgtcccgccc
     1381 ggccgccggg cggtcagcgc gcagggcgag gcctacgacc actacgcgat cgcgcagatg
     1441 gaggcccgcg ccggccggat gccgcaggcc atcgcccagc tccgcgaggc catcaagctc
     1501 gatcccaaca ccgcggccct ctggatccag ctctcccagt ggttgatccg gagcaacgac
     1561 accgcgggcg ccttcaccgc cgcccagaag gcggtcgcgc tcgagcccaa caacgcgggc
     1621 gcccacatga cgatggccga cctctacaag cgccagcgca agccggcgga ggcggaggcc
     1681 gagctggagc gggcggtggc gctcgaccct cagacgccgg acggctacct ggccctggcc
     1741 cagctccact tcgagggcaa gagctacgac aaggcacgcg cggtactgcg gcggctggtg
     1801 accgcgcgcc cgaatctctc gcagggctac tacctgctgg ggcgcatcgg catcgagaac
     1861 gagcagtggg acgaggccct gaccaatctc aagcgcgcgg tggagctcga ccccgatcac
     1921 gacggggcgt ggtccgcgct cggcttcgtc tacgaagccc agaacaagct cgacgacgcg
     1981 gtcgcggtct acaaggacgc gctcaaggcc aacccggaca acgcggcctt cgtggagcgg
     2041 ctgggcgacc tgctcgtgaa gctcgggcgc tacggcgagg cccagagcga gatcgagtcg
     2101 ctcgtcgact ccctgccgcg cgatccacgg gtgtggctga agctgggcgc gatccactac
     2161 gagcagaagc agtacgacaa ggcggtcgcc gccttccgcc aggccgtcgc gctcgagccc
     2221 ggcaacatgc ggacgcgcta ctacctggcc accgcgctga tggactcggg caaggacgac
     2281 gaggccctga gcgagctgga gaagatcctc aaggccgatc cccgctcggt ggacgcgcgc
     2341 gtgcagctcg ggttcctcta cgggcgtgcc aagcgctacg acgaggcggt gggcacgctg
     2401 caggaggcgg tgaatctcga gcccaagcgc cccgagctct tcctctacct gggcaccgcg
     2461 ctccagcgcg ccagccagta cgatcgctcg gcgagcgtcc tgcgcgaggg gctgtcggtg
     2521 gacgacaaga acaaggatct ccacttccag ctcggggtgg tgctggagaa gcagcatcag
     2581 ttcgacgacg ccatccagtc gttccgccgc gtgctcgtgc tggatcccaa gcacgccgag
     2641 tcctacaact acatcggcta catgtacgcg gagaagggca cgaacctctc cgaggccatc
     2701 cagctcatcc agcaggcgct cgcgctcgag ccccagaacg gctacttcat cgacagcctg
     2761 ggctgggcct actaccagca ggggcgctat cccgaggccc tgcgcgagct gaagcgcgcg
     2821 gtgagcctgg ccaaggaaga tcccgtgctc tacgagcacc tgggcgacgc ctacctcaag
     2881 aacaacctcg cgaacgaggc catcgaggcg tgggagaagt cgctccaggt ggatccgaac
     2941 gcccccaccg ccccccaggt gaggaacaag atccgggacg cgcgggagaa ccagctccgg
     3001 gtcaagggtg cgccgcccaa ggccgagcag aagtagcggg ctccgcctgg ccggcgggct
     3061 cctgctggcc gccagcctgt ccgcctgcgc cacagctcca ccggcgcccg cgccaccgcc
     3121 catcgccgag gaagcccgcg cggccctggc ccgcatcgag cagcatcgcc gctcgctggt
     3181 cgatctccgg tccctggccg acatcacggt gcgccgcggc gggcgggcgc aacggctctc
     3241 cggggtcatg ctgctcctgc gtgagacctc ctcgctccgc ttcgaggccc tctcgccctt
     3301 cggcatgccg gtgctgatcg tggccggcga tcctcagagc ctcacgctct gggaggtcct
     3361 ggacaaccgg gcgtatctcg cccccgcctc ccccgaggcc aaccgccgct ggctcggcct
     3421 ggcgctgggc ggggaggatc tcgtcgcgct gctcgcggcc cgcgcccggc ctctgccgga
     3481 cccgacccgg gtggagatgg tgggcccgga cgagaccggc ccctcggtcc ggctcaccgg
     3541 gcgcgacgtc gagcagcgca tctggttcga tcccgccagc gggcaggcgc gacaggtgga
     3601 gtggacgggc aagaacccgg cccgggtcac tttcactcca acgcccccgg aggccccgcc
     3661 ggccgggctc cgcctcgcga cgccggacgg cacgctgcgg gtctcggtca cctaccggga
     3721 ccccaagatg aacacgggac tcgaccccga gctgttgaag ctgactgtcc cggaacacgt
     3781 tagaattcaa gacttccgtt gacactcttt cgcggcacat ggtagcctac gctgccttgt
     3841 cgaagcggtc acgggggagc atggaggtgg tgcttcgcac ctccgccaaa gtgaacctgg
     3901 ccctcgaggt gctggggaag cggggcgacg ggtatcacga gatcgcgacg gtgctgcagg
     3961 cggtggacct gttcgatcgc ctgaagctgg aagcggccga caccctttcg ctgcacacgg
     4021 acgatcccga cttgcccacc gacgatggca atctggtgat gcgcgccgcg cgtctgctcc
     4081 agaaggcggc ggggatcgag gccggggcgc gaatccggct gaccaagcgc atcccggtgg
     4141 cggccgggct gggaggcggg tcgagcgatg cagcggcgac gctcttgggg ctccaccggt
     4201 tgtggcggct ccgctggccc cgagcgcggt tgcaggagct ggcggtggag ctgggcatgg
     4261 acgtcccgtt cttcctgggg tcggggcgcg cggtggccag agggcgcggc gagcagctga
     4321 ccgcgctgcc cggcggtggg ggctacgctc tggtgctggt gaacccccgc gtgccgctgt
     4381 ccacgcggga ggtgtacggt cgggttccgg cgggctggcg agccgagccc accggcacgg
     4441 agcgggtgat cgaggcgctg aggacgcgca acgtgagcaa gatggcggcg gcgctgacga
     4501 acaatctgga gagcgtggtc gagccggtgc tgccggtgat cggccgcatg aaggcggccc
     4561 tcttggcggc gggcgcgctt ggagcgatca tgtcgggcag cggaccgacc gtgttcggga
     4621 tggcgcggtc cctggaccac gcgcggcaga tccggacgcg ggtgagccgg gcggagtggg
     4681 cgtgctgggc ggtgcggacg aactcgggcc cagccatccg ggtgagccga tgaggctgaa
     4741 cgcgatcata ctggggcgtc gttcagtggt aggactcggg gctttggccc ccgcaatgga
     4801 ggttcgattc ctcccgcccc agccacatac gcggctcgag gcgcagcggc ttggcgaggt
     4861 gaagccgaag gcgagccgaa cggatatgaa gagaagcaag gcaggtacgc ccaagcgggc
     4921 ggccccgatg gccccatcgc cacaggagac gcaatggcct acgagctgaa gctcttctcc
     4981 ggcaacgcga accgcccgct ggcggaggag atcgccaaga ctcttcagct cccgctgggc
     5041 gacgcggacg tctcgcgctt ctccgacggc gaggtgtacg tgcagatcaa cgagaacgtc
     5101 cgggggcagg acgtcttcgt gatccagccc acctgcccgc cggtcaacga tcatctgatg
     5161 gaactgctcg tgatgatcga cgcgttcaag cgcgcgtcgg cccggcgcat caccgcggtg
     5221 ctgccctact acggctacgg ccgccaggac cgcaaggtgc agccgcgcgt accgatcacc
     5281 gcgaagctgg tggccgacct cctgaccgcc gcgggggccc aacgcctgct cgcggtggat
     5341 ctccacgccg ggcagatcca gggcttcttc aacatcccgg tggaccacct cttcgcggcg
     5401 cccccggtca tcatcgacca tctggccaag aaggacctga acgacccggt gctggtctcg
     5461 cccgacgcgg gcggcgtgga gcgcgcccgc gccctcgcca agcggctcaa ggcgggtctc
     5521 gccatcatcg acaagcggcg cgagggcaac aacgtcgccc agttcatgca cctcatcggc
     5581 gacgtcaagg gccgcgacgc ggtgatcgtg gacgacatga tcgacaccgc ggggaccatg
     5641 atccaggcgg tggacgcgct ccggcgcgag ggggcgaacc gggtcctcgc ctgcgcggtg
     5701 cacggcgtgc tctccggtcc cgccatcaac cgcatcaagg aatcgatcct ggaggaagtc
     5761 atcatcacga acacggtccc gctcacgccc gagcggcagc tgtccaagat ccgcgttctc
     5821 tcggtcgccc cgctgctcgc ggaggcgatc cgtcgcatcc acgacgagga atccgtctcg
     5881 accctcttcg tgtaatacaa acttcgagct taaggagctg agtcatggaa atgcgtgagt
     5941 tgaccatccg gaagcgcgag ggcatcggca agggccccgc ccggcgtctg cgccgcgcgg
     6001 gcgaggtgcc ggcgaccctc tacggcggcg cgagcccggt gaacgtggcg gtgatgccca
     6061 aggacgtgct ccgtctcatc cacggccgag agggcagcac ccagctgttc cgcgtgacct
     6121 tcgaggggac cacggacggc aagatggcga tcatccggga catgcagttc gatccggttt
     6181 cggagaatct cctccacgtg gacctgcagg aagtggcgat ggatcgcgcc atccaggtca
     6241 cggtggccgt gcaccacgtg ggcgaggcga tcggcgtcaa ggacacccag ggcatcctcg
     6301 agatggtgct ccgcgaggtg cgggtctcct gcttgcccgg gaacatcccg gagcacctcg
     6361 aagcggatat cacgaacctg gccatccacg acgtgctgac cgtgaaggac ctgaaggtcc
     6421 ccgagggcgt gcgcgtcctc aacgacccca accaggcggt cgtcacggtc gccccgccgg
     6481 ctgccgagga agcggtggcg gcggccgcgg tgccgacgga agcggcggcg gccgagcccg
     6541 aggtgctcac cgagcgcaag cccaaggagg gggaagcagc cgagggcgac gcgaaggaca
     6601 agggcggcga gaagaagaag taggtggccc aggccatcgt cggcctgggg aatccgggcc
     6661 cggaatatcg caacacccgg cacaacgtgg gccagcgcgt cgtcgacgcg ctggcccacc
     6721 gcattcatgc gcgcttcgag cgcgacagcg ggcacgcggt cgcgcccggc cgctggcacg
     6781 gcgagcccct ctacctcatc aagccgggca gcttcatgaa cgtcaccggc gccccggtgg
     6841 cgcggctcgc ccgcaagctc cacctgggcc ccgccgacct catcatcgtc ttcgacgatc
     6901 tggatctccc gctgggcaag gtccgcgtgc ggctcaccgg cagcgcgggc ggccacaacg
     6961 gcgtccgctc gctgatcgcg gccttcggca ccaacgacct ccgtcgcgtc aagatcggca
     7021 tcggccggcc cggccggccc ggcctagacc gggaccgcgt ctccgatcac gtgctctcgc
     7081 ccttctttcc cgaggagcag gaggcgatcg ccgcggcctg cgacgaggcc gcggacgaag
     7141 cgcttgccct cgtggagcgc cgctgagggc cccccgcgca tgagccccgc gttccggctc
     7201 tccgcgggca gcggcggcct cgagtggccg caggtgctgg cgctcctcgc ccgcgaggcg
     7261 cggacgccga tgggccggga gctggcgacc gccgcggttc cgttcacgga ccccggcgcg
     7321 atccggcgtg cgctcggcga gacccgccag gcccgggccg cgatgggcca gaccggcgtg
     7381 ccgcccttgg agggcatccc cgacgtgcgg cccacgctcg agaccgcgcg cgtggcgggc
     7441 tcggtggccg aggccgcgga cctggccgcg ctgatcccgc tcctcgaggc ggcggggcgc
     7501 ctgctcgcct acggccgcgc catccagccg gtggcgcccg ctctcgcgga ggcgctcgcg
     7561 ggcttcccga gccagaccga gctggccgac ctgctccggc gctcgctcga cgccgacggc
     7621 caggtgcgcg acgaggcggc gcccgcgctg cgccgcgtcc gccagcgcat ccgcgacctg
     7681 cggcgcgatc tcgtcaagcg gctcgaggcc tacttcgccg cgcccagtgc cgacaccacg
     7741 ttccaggagc gctacgtcac ggtccgccac ggccggtacg tgctgccgat ccgcgcggag
     7801 gccaagggcc gcctgcgcgg catcgtccac gaccgctcgc agagcggagc cacgctcttc
     7861 gtggagccgg aggcgctggt agaggccaac aacgacctcg ttcaggccac gcgcgaggaa
     7921 gagaccgaga tcctccgcgt cctggccgcg ctgaccgacg cggtgcgggc ggcgctgccc
     7981 gagctcgacg acctggtggc gggcatcggc ggcctcgacc tgatcttcgc ccgcggcgcg
     8041 ctcgccgagc gcatggaggc cgtcgagccc acgatcgccg acgagcgcga cgtgctcctt
     8101 cgcggcgccc tcaatcccct ccttcttgcc cagagctggg cgaccgaggg cggccgaccc
     8161 gtcattccca tggacctcga gatcggatcc gagcgtccgg tgctcgtcat caccggcccc
     8221 aacgcgggcg gcaagacggt ggcgctcaag accctgggcc tgctcgtgct catggcgcag
     8281 gcgggctgcc acgtgcccgc gcgcgacggg gcgcggctgc ccgtcttctc gcagtgcttc
     8341 gccatcgtgg gcgacgagca gagcgtggcg gagaacctct cgacgttctc cgccttcgtc
     8401 aagcagctcc gcgaggtcct cgcgcgcgtg gacgcccgct cgctcgtcct gctcgacgag
     8461 ctgggcgcgg gcaccgatcc cgacgacggc gccgcgctgg cccaggcggt gctggaggac
     8521 ctggccgagc gcggcgcggt ggtcgtggcc tccacccacc tcgagccgct caagggcttc
     8581 gcctccacgc acccgagggc ccgcaacgcc tcggtggagt tcgatcccga gcggctcgcc
     8641 ccgaccttcc ggctcatcta cgatcgcccg ggccagagct acgcgctctc catcggcgcc
     8701 cgcctgggcc tgcccgccgc gctgatcgcg cgggcacacg cgcatcgctc cacccagcag
     8761 cggcagctcc aggagctgct ggcccgcctc gacgaccggg accgcaagga cgcggagcgc
     8821 cgcgcgcagc tcgagcggcg cgaggcggag agcgcggggc tgctcgcgcg ggcccaggcc
     8881 gagctcgagg cggcgcgggc gagcgcgcgc gagaccatcg cccgggccaa ggcggaagcg
     8941 cagcgcctgg tcaccgaggt gcgacggcac gtgaacgagg agtgggaccg gctcaagcgc
     9001 gccgagaaga gccggccgga gctggagcgc gcgcgcaagc gact
//
LOCUS       DASUFA010000357         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_420324, whole genome shotgun sequence.
ACCESSION   DASUFA010000357 DASUFA010000000
VERSION     DASUFA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420324"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFX87_13070"
     CDS             <1..952
                     /locus_tag="VFX87_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX4995905.1"
                     /translation="TKRVRIASSAGVLVEDVQPLFSIRVSVIASEGGVRREGSAGGGG
                     RVAFDFFLEQDRWKRFTGEAVRQALMKLDAVDAPAGEMTVVLGPGWPGILLHEAVGHG
                     LEGDFNRKKTSAFSGRLGQSVASELVTVVDDGTIKNRRGSLNVDDEGVPTRRNVLIEK
                     GILRGYLQDRLNSRLMGMEVTGNGRRESYAHPPMPRMTNTFMLAGEDEPADIIRGVAR
                     GLYAVNFGGGQVDITNGRFVFSASEAYLIEDGRVTRPVKGATLIGNGPEALTRVSRVG
                     HDLELDEGVGTCGKDGQSVPVGVGLPTIRIDRMTVGGTQV"
     gene            958..2292
                     /locus_tag="VFX87_13075"
     CDS             958..2292
                     /locus_tag="VFX87_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX4995906.1"
                     /translation="MRELLVDLLQQATRRGATAADAFVVEDQSFSAQVRLGQVDTVKH
                     AREQHMALRVFVGKSVAAASTSDLSPESMARLVDEAVSLARITSPDEMGGLPEAGELA
                     RQVPDLDLRDAHGHDLSPEEKIELARRCEAAALEADPRITNSEGGDFSDRRARYAYAT
                     SHGFAGEYATSSFSLAVSPVATANGEMQRDSWYHVTRKRALLEAPEEIGRIAARRAVR
                     RLGARQVKTAEVPVIFDPEMAASLVRHIAGAAAGPALYRRASFLVGKLDERIAAPSVT
                     IVDDGTIPGALGSRPFDGEGLPVRRTVVVEQGVLRSYLLDTYSGRKLGLPSTHHAARD
                     GSGVSVSTTNLHLKAGNQDPADLIRSVKSGLYVTELIGFGVNGVTGDYSRGAVGLWIE
                     NGELAYPVEEITIAGNLLDMFHAVEGIGNDLVFRDRTAAPTLLIGKMTVAGA"
     gene            2289..3071
                     /locus_tag="VFX87_13080"
     CDS             2289..3071
                     /locus_tag="VFX87_13080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4995907.1"
                     /translation="MIIDSHCHLHDPAFADVRGTVIRATEHNVWGAVAVGCDHATNER
                     TLQAAAENSKAVWPALGLHPEWKLTREDLEGVEQQIHANHARIVAVGEVGLPWYGLGA
                     AADADAVRREARERLERLAALACRYDLPVILHAPHGAAADALAILKRRGVERAVFHWH
                     KASAEVTREIVDAGYLVSVTPDLVYRDRDREMVERVPIESLLVESDAPWQYQGEFENV
                     TSGPWLVARVAEEVAKIKRLPVDEAMYQLTVNTCRVFDIVWA"
     gene            3068..>3638
                     /locus_tag="VFX87_13085"
     CDS             3068..>3638
                     /locus_tag="VFX87_13085"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995908.1"
                     /translation="MSEQRFTVPVGADDVAGVLHLPDDCRPGPDRGPVPSPCVIACHG
                     MGASKDSDKYLLLGRELPAAGLALARFDFRGSGESAGLQRDATIETRIADLEAVLGHL
                     AAQPELDGRFGLLGSSLGGYVALWAASRRAPGTPLPVVTWNAPAALRELAHADPGEAG
                     AALVAEVKGGMRAEAPAGVARVLVIQADRD"
BASE COUNT          522 a         1266 c         1362 g          488 t
ORIGIN      
        1 gacgaagcgg gtccgcatcg cgagctccgc cggcgtcctc gtcgaggacg tgcagcccct
       61 cttctcgatc cgggtgagcg tcatcgcgag cgaggggggc gtgcggcgcg agggctcggc
      121 ggggggaggc gggcgcgtgg ccttcgactt cttcctcgag caggatcgct ggaagcgctt
      181 caccggcgag gcggtgcgcc aggccctcat gaagctcgac gcggtggacg cgcccgcggg
      241 cgagatgacg gtggtgctcg ggcccggctg gcccggcatc ctgcttcacg aggcggtggg
      301 ccacggcctc gagggcgact tcaaccgcaa gaagacctcc gcgttctcgg gacggctcgg
      361 ccagtcggtg gcctcggagc tggtgacggt ggtggacgac ggcacgatca agaaccgcag
      421 aggctcgctc aacgtggacg acgagggcgt gcccacccgg cggaacgtgc tcatcgagaa
      481 ggggatcctg cgcggctacc tgcaggaccg gctcaactcg cggctcatgg gcatggaggt
      541 caccggcaac ggccggcgcg aatcctacgc gcatcctccg atgccgcgca tgaccaacac
      601 cttcatgctg gcgggcgagg acgagcccgc cgacatcatc cgcggcgtcg ccagggggct
      661 gtacgcggtg aacttcggcg gcgggcaggt ggacatcacc aacggtcgct tcgtgttctc
      721 ggcgagcgag gcctacctga tcgaggacgg ccgcgtgacc cgcccggtga agggcgcgac
      781 cctgatcggc aacgggcccg aggcgctcac gcgggtgagc cgggtcggcc acgatctcga
      841 gctcgacgag ggggtcggca cctgcggcaa ggatggccaa tcggtcccgg tgggcgtggg
      901 actgccgacg atccgcatcg atcgcatgac cgtcggcggg acccaggtct gagccgcatg
      961 cgcgagctgc tcgtcgatct cctgcagcag gcgacgcgtc ggggcgcgac ggccgccgac
     1021 gccttcgtgg tggaggacca gtcgttctcg gcccaggtgc gcctgggcca ggtggacacc
     1081 gtcaagcacg cgcgcgagca gcacatggcg cttcgggtct tcgtgggcaa gtcggtggcg
     1141 gccgcgtcca cgtccgatct ctcgcccgag tcgatggccc ggctggtgga cgaggcggtc
     1201 tcgctcgctc gaatcacgag cccggacgag atgggcggcc tgcccgaggc cggggagctc
     1261 gcgcggcagg tgccggacct cgacctgcgc gacgcgcacg gccacgacct cagccccgag
     1321 gagaagatcg agctggcccg ccgctgcgag gccgccgcgc tcgaggccga tccccgcatc
     1381 accaactccg agggcggcga cttcagcgac cggcgcgcgc gctacgccta cgccaccagt
     1441 cacggcttcg cgggcgagta cgcgacgtcc tcgttcagcc tcgcggtctc cccggtggcg
     1501 accgcgaacg gcgagatgca gcgcgactcc tggtaccacg tcacccgaaa gcgggcgctg
     1561 ctcgaggcgc cggaggagat cgggcgcatc gcggcgcggc gcgcggtgcg ccggctcggg
     1621 gcgcggcagg tgaagaccgc ggaggtgccg gtgatcttcg accccgagat ggcggccagc
     1681 ctcgtgcggc acatcgcggg cgcggccgcc ggccccgccc tctaccggcg cgcctccttc
     1741 ctggtgggca agctggatga gcgcatcgcg gctccgtcgg tcaccatcgt ggacgacggg
     1801 accatcccgg gcgccctcgg ctcgcgccca ttcgacggcg aggggctgcc ggttcgccgc
     1861 accgtggtgg tggagcaggg cgtgctccgc tcctacctgc tcgacaccta cagcgggcgg
     1921 aagctcgggc tgccgtccac ccatcacgcg gcccgcgacg gcagcggggt gagcgtgtcc
     1981 accaccaatc tccatctgaa ggcggggaac caggatccgg cggacctgat ccggagcgtg
     2041 aagagcggtc tctacgtcac cgagctgatc ggcttcggag tcaacggggt caccggggac
     2101 tactcgcgcg gggcggtggg cctctggatc gagaacggcg agctggccta tccggtcgag
     2161 gagatcacca tcgcgggcaa cctgctcgac atgtttcacg cggtggaggg catcggcaac
     2221 gacctcgtgt tccgggatcg caccgccgcc ccgaccctgc tcatcgggaa gatgacggtg
     2281 gccggcgcat gatcatcgac agccactgcc acctccacga cccggccttc gcggacgtcc
     2341 gcggcaccgt catccgcgcc accgagcaca acgtgtgggg cgcggtggcg gtcggctgcg
     2401 accacgccac caacgagcgg acgctgcagg ccgccgcgga gaacagcaag gcggtgtggc
     2461 cggcgctggg tctccatccc gagtggaagc tcacccgcga ggacctggaa ggggtggagc
     2521 agcagatcca cgccaaccac gcgcggatcg tcgcggtcgg cgaggtgggg ctgccgtggt
     2581 acggcctcgg ggcggccgcc gacgcggacg cggttcgccg agaggcccgc gagcgcctcg
     2641 agcgcctggc cgcgctggcc tgccgctacg atctgccggt catcctccac gcgccccacg
     2701 gggcggccgc ggacgcgctc gcgatcctca agcggcgggg cgtggagcgc gcggtgttcc
     2761 actggcacaa ggcctcggcg gaggtcaccc gcgagatcgt ggacgccggc tatctcgtgt
     2821 cggtgacgcc ggacctggtc taccgcgacc gcgaccgcga gatggtcgag cgcgtgccga
     2881 tcgagtcgct gctggtcgag agcgacgcgc cctggcagta ccagggcgag ttcgagaacg
     2941 tcacctcggg gccgtggctg gtcgcacgcg tggcggaaga agtggcaaag atcaagcggc
     3001 tgccggtgga cgaggcgatg taccagctca ccgtcaacac ctgccgcgtc ttcgacatcg
     3061 tctgggcgtg agtgagcagc gcttcacggt gcccgtcggc gccgacgacg tggccggcgt
     3121 gctgcacctg cccgatgact gccgcccagg gccagaccgc gggccggttc cgagtccctg
     3181 tgtgatcgcc tgtcacggca tgggcgcctc caaggacagc gacaagtacc tgttgctcgg
     3241 gcgcgagctg cccgcggccg ggctcgcgct cgcgcgcttc gacttccgcg gcagcgggga
     3301 atccgccggg ctccagcgcg atgccaccat cgagacccgg atcgcggacc tggaagcggt
     3361 cctgggccac ctggccgcgc agccggagct cgacggccgt ttcggcctgc tgggctcgag
     3421 tctcggcggc tacgtcgccc tctgggccgc gagccgccgg gcgccgggga cgccgctgcc
     3481 ggtggtgacc tggaacgcgc cggccgcgct gcgcgagctg gcccacgccg atccgggaga
     3541 agcgggcgcg gccctggtcg ccgaggtcaa gggcggcatg cgggccgagg cgcccgcggg
     3601 cgtggcgcgc gtcctcgtca tccaggccga ccgggacg
//
LOCUS       DASUFA010000358         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_420972, whole genome shotgun sequence.
ACCESSION   DASUFA010000358 DASUFA010000000
VERSION     DASUFA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420972"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFX87_13090"
     CDS             complement(<1..542)
                     /locus_tag="VFX87_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995909.1"
                     /translation="MAGITFDRREQDLGNILFLEHVNVRVPDQHAATLFYIAGLGLTR
                     DPYLMVSVENMWVNAGQTQFHLPTGAPNVLQGHVELVVPDLDALMRRLAAVREPLAGT
                     RFAYEMRDKHVAVTCPWGNQFRCVGPAPEYGDMTLGISRVEVPVARGTAAGIASFYKT
                     ALAATASVSQDDAGLAARISV"
     gene            complement(582..797)
                     /locus_tag="VFX87_13095"
     CDS             complement(582..797)
                     /locus_tag="VFX87_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995910.1"
                     /translation="MDLNTYATEKIAAGRLADLRAASARVALIESAGVGPRGVGPVVG
                     SALIRLGHWLAPDEAVAAPNAGVRVAR"
     gene            complement(820..1227)
                     /locus_tag="VFX87_13100"
     CDS             complement(820..1227)
                     /locus_tag="VFX87_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995911.1"
                     /translation="MNLETEITMIMIALLPFLAVWALLDVSRRVQRRREEQVARQVAL
                     TDAIHRELGGAAAPEVTRSLRGTWTVSMRLPLHREDVVGVITRLTQDVFRRLDRQDPP
                     RLRLVLIPQTVRPWRRARVADSTRAAGRLSRAA"
     gene            complement(1330..2295)
                     /locus_tag="VFX87_13105"
     CDS             complement(1330..2295)
                     /locus_tag="VFX87_13105"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4995912.1"
                     /translation="MSPQHPLCRAIEPDLLSVAAGEAGVAAAGRVEDHVATCGLCRGE
                     LARYRALEGMVDSLRRAPLPDDDATLARAQLASRLSDLRSRMVGFGIFASPLGRILIG
                     RSEQGVALVQYLPADGPLSAHVRRLLGEDAVEDRAATEGLRAELVEYLEGRRARLDWP
                     LDLRRMRSAFQRRVLEATSALPYGAVTSYAGIAARIGAPSAVRPVAQALRWNPLPIVI
                     PCHRVIGASGALTGYAGRRVELKQRLLAVEGVKTVAARNDFKVNREAMYTMVTGDREY
                     CVPTCGSLLSQSLSTLTLFGTREGAEAAGFAPCTSCRPDLHPLPA"
     gene            complement(2292..2819)
                     /locus_tag="VFX87_13110"
     CDS             complement(2292..2819)
                     /locus_tag="VFX87_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4995913.1"
                     /translation="MAEDAFEAVVGAHHAEIRRYLLRITARGSDADDLSQETFLRAFR
                     AYRALPPGSNVRAWLFAIATNLGRNHFRAQGRRRRAYAAAALEMRPDAPDRADGQVLA
                     DEARERLERVISGLPGKQRLAFIMRKMHELEYAEIGASLSCSEESARAHVFQALRKVR
                     RSLDGHLPVGMESRS"
     gene            complement(2877..3848)
                     /locus_tag="VFX87_13115"
     CDS             complement(2877..3848)
                     /locus_tag="VFX87_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995914.1"
                     /translation="MTGLRWSHLIFLLSTWAVVSFLALVVVGLAWTIRLKPQALADAR
                     AQRQEIVRVLARLGDAQVRDKLDALGPLAAHFAQSWASHEPRALQREIDNLRAVLQMP
                     TLFVVSPDGRPLAFSPLTGPDGASNLDLRYTDRPLLQEAIRVQGPAVSGIIFGRASRD
                     FIVGAAVPIKLDERIVAYLLGSIRLQASIEAIEQAGPGGWLVMIGADKQAIYLDRQSR
                     QITQEDWTSHPFVEELGRRQSDGLVSLGEDEWLLTQAVMPTLKVNLVFAASVRQILEN
                     ERAVLLTLVATLLVSVAMSLAVSTVTAVRFVREQQAAALKTRMGTGG"
     gene            4001..>4987
                     /locus_tag="VFX87_13120"
     CDS             4001..>4987
                     /locus_tag="VFX87_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX4995915.1"
                     /translation="MKAAVLYKANTPLEVVDLVQEGPKAGEARVKVKAAGICHSDWHI
                     MNGDWTPPLPMVLGHEAAGMVEEVGPGVTNVKPGDHIIFSFRPHCGRCLYCSMGRSIL
                     CDGHASPRWGMLDGTYRLKRNGQDIYQMARIGTFSEQVVCPAEMLVPIRKEMPWPQAA
                     LMGCCVPTGVGAVTRCAEVEAGASVLVIGCGGVGLNVVQGARLAGAGIIAAADLLDSK
                     LAYAKDFGATHTINAKSENVVDRVRELTPGGRGVDYAFDAIGGEATTLQIVEAIRPGG
                     TAVIVGMAALSVRAPITPYMMALQEKAIKGTMYGSVRPNIDFPKFVDLYLEGR"
BASE COUNT          750 a         1799 c         1754 g          684 t
ORIGIN      
        1 acgctgatgc gcgcggccag accggcgtcg tcctggctca cggaggcggt ggccgccagc
       61 gcggtcttgt agaaggacgc aatgcctgcg gcggtgccgc gcgcgaccgg tacttctacc
      121 cgcgagatgc cgagcgtcat gtcgccgtac tccggcgcgg gacccacgca gcggaactgg
      181 ttgccccacg ggcaggtcac cgccacgtgc ttgtcgcgca tctcgtaggc gaaccgggtg
      241 cccgccagcg gctcgcgcac cgcggccagg cgccgcatca gcgcgtcgag atccggcacc
      301 accagctcga cgtgcccctg cagcacgttg ggcgcgccgg tgggcaggtg gaactgggtc
      361 tgcccggcgt tgacccacat gttctccacg ctcaccatca ggtagggatc acgtgtcagc
      421 ccgagccccg cgatgtagaa cagcgtggcc gcgtgctggt cggggacccg cacgttcacg
      481 tgctccagaa acaagatgtt cccgagatcc tgctcccgcc gatcgaacgt gatccccgcc
      541 atggccccgg ccctccccgg cctgggggca agcatactat ctcagcgggc gacccgcact
      601 cctgcattcg gcgccgcgac tgcctcgtcc ggggccagcc agtgcccgag ccggatgagg
      661 gcggacccga cgacgggccc caccccgcgc ggcccgaccc cggccgattc gatcagggcc
      721 acgcgcgcgc tggccgcgcg cagatcggcc agacgccctg cggcgatctt ctcggtggcg
      781 tacgtattga ggtccatgac cgctctcctt tccctggtct caggccgccc ggctgagccg
      841 gccggctgcg cgggtcgagt ccgccacccg ggcccgccgc cacggacgga ccgtctgcgg
      901 gatcaggacc aggcgcagcc gtggcggatc ctgccggtcg agccgccgga agacgtcctg
      961 ggtgagccgc gtgatgacgc cgaccacgtc ctcgcgatgg agcggcagcc gcatcgagac
     1021 cgtccacgtg cccctcaggc tccgcgtgac ctcgggcgcc gcggcgccgc ccagctcgcg
     1081 gtggatggcg tcggtcagcg cgacctgccg cgccacctgc tcctcgcggc gtcgctggac
     1141 gcgacggctc acgtcgagca gggcccacac cgccaggaat ggaagcagcg cgatcatgat
     1201 catcgtgatc tccgtctcca ggttcatcat ttcctcctcc ggtcgttatt ccgtctcttc
     1261 gcccgtttct tcagacactg agacaccaag gacgagacgg tgtgaaaagg cggaggacga
     1321 cgcggccggt caggccggga gcggatgcag atcgggccgg cagctggtgc agggcgcgaa
     1381 gccggcggcc tcggcgccct cgcgcgtgcc gaacagcgtg agcgtggaca gcgactggga
     1441 caggagcgag ccgcaggtcg gcacgcagta ctcgcggtcg ccggtgacca tcgtgtacat
     1501 cgcctcgcgg ttcaccttga agtcgttccg cgccgcaacc gtcttcacac cctcgaccgc
     1561 gagcaggcgc tgcttgagct ccacgcgccg gcccgcgtag ccggtcagcg ccccggaggc
     1621 gccgatgacc cggtggcaag ggatgacgat cgggagcggg ttccagcgca gcgcctgcgc
     1681 gaccgggcgc accgccgagg gcgcgccgat gcgcgcggcg atccccgcgt aggaggtgac
     1741 ggcgccgtag ggcagtgcgg atgtcgcctc gagcacgcgc cgctggaagg cgctccgcat
     1801 ccggcgcagg tcgagcggcc agtcgagtcg ggcgcgccgg ccctcgagat attccaccag
     1861 ctccgcccgc aacccttccg tcgcggcgcg gtcctcgacc gcgtcctcgc ccaggaggcg
     1921 gcggacgtgc gcggacagcg ggccgtccgc gggcaggtac tgcaccaggg ccacgccctg
     1981 ctcggagcgg ccgatgagga tgcgcccgag cggcgacgcg aagatcccga agccgaccat
     2041 gcgcgagcga aggtccgaga ggcgcgaggc gagctgcgcg cgcgccagcg tcgcgtcgtc
     2101 gtcggggagc ggcgcccggc gcagcgagtc caccatgccc tcgagggcgc ggtagcgcgc
     2161 gagctccccg cggcagagcc cgcaggtggc cacatggtct tccacgcgcc cggcggcggc
     2221 gacgcccgcc tcgccggcgg cgacactcag gaggtccggc tcgatggcgc ggcacagggg
     2281 atgctgaggg ctcatgaccg ggactccatt ccgaccggga gatgaccgtc gagacttcgc
     2341 cgcaccttgc gcagggcctg gaagacgtgg gcgcgggcgc tctcctccga gcaggagagg
     2401 ctcgctccga tctccgcata ttcgagctcg tgcatctttc tcatgatgaa ggccagccgc
     2461 tgcttcccgg gcaacccgct gatcacccgc tccaaccgct cgcgcgcctc gtcggccagc
     2521 acctggccgt cggcccgatc cggcgcgtcc ggcctcatct cgagggccgc cgccgcgtag
     2581 gcccggcgcc ggcgcccctg tgcccggaag tgattccggc cgaggttggt ggcgatcgcg
     2641 aagagccacg cgcgcacgtt cgagcccggc ggcagggcgc ggtacgcccg gaacgcgcga
     2701 aggaacgtct cctgcgagag atcgtcggcg tcgctacccc gcgccgtgat ccgcagcagg
     2761 tagcgccgga tctccgcgtg gtgggctccc accaccgcct cgaacgcgtc ctctgccatc
     2821 actctagcca tgagacaccg ccgccggccg ggtgtgaaac ggccccacgg cgcgcgctaa
     2881 ccccctgtcc ccatgcgcgt cttgagcgcg gccgcctgct gctcccggac gaagcggacg
     2941 gcggtcaccg tgctcacggc gaggctcatg gccaccgaga ccaggagggt ggcgaccagg
     3001 gtcaggagca ccgcgcgctc gttctccagg atctggcgca ccgaggcggc gaagaccagg
     3061 ttgaccttga gggtcggcat caccgcctgg gtgaggagcc attcgtcctc gccgagggac
     3121 acgaggccgt cggactggcg gcggcccagc tcctccacga aggggtggct cgtccagtcc
     3181 tcctgggtga tctggcggct ctgccggtcc aggtagatcg cctgcttgtc cgcgccgatc
     3241 atgaccagcc agcccccggg gcccgcctgc tcgatggctt cgatggacgc ctgcagccgg
     3301 atgctcccga gcaggtaggc cacgatccgc tcgtccagct taatcggcac cgcggcgccc
     3361 acgatgaaat cacgcgaggc ccggccgaag atgataccgg agaccgcggg accctggacc
     3421 cggatggcct cctgcagcag cggccggtcc gtgtagcgca ggtcgaggtt ggaggcgccg
     3481 tccggcccgg tgagcgggct gaaggcgagc ggacggccgt cgggggacac cacgaagagg
     3541 gtcggcatct gcagcaccgc ccgcaggttg tcgatctcgc gctggagcgc acgcggctcg
     3601 tgcgaggccc agctctgggc gaagtgggcg gcgagcgggc cgagggcgtc gagcttgtcg
     3661 cgcacctggg cgtcgccgag ccgcgcgagc acgcgcacga tctcctggcg ctgcgcgcgt
     3721 gcgtcggcga gcgcctgcgg cttgagccgg atcgtccacg cgagccctac caccacgagg
     3781 gccaggaacg acaccaccgc ccaggtggag agcaggaaga tcaggtgcga ccagcgcagt
     3841 cccgtcatga gactcccgtc atcgagggct gatgataccg cgcttgacag ggcgcgcgcc
     3901 ccgccgcatc atcggccgcg cagcgccccg tccctcggag ggagcacatg gccgacggtg
     3961 tcagcgcccg ttccctaact tcagccagga ggagatcacc gtgaaagccg ccgtcctcta
     4021 caaggccaat actccgctcg aagtggtcga tctcgtccag gaagggccga aagccggcga
     4081 ggcccgcgtg aaggtcaagg ccgccggcat ctgtcacagc gactggcaca tcatgaacgg
     4141 ggactggacg ccgcccctgc cgatggtcct gggccacgag gcggccggca tggtcgagga
     4201 ggtggggccg ggcgtcacca acgtcaagcc cggcgatcac atcatcttct cgttccgccc
     4261 gcactgcggc cgctgcctct actgctcgat gggacgctcc atcctctgcg acgggcacgc
     4321 ctccccgcgc tggggcatgc tcgacggcac ctatcggctc aagcgcaatg ggcaggacat
     4381 ctatcagatg gcgcgcatcg gcaccttctc cgagcaggtg gtgtgcccgg cggagatgct
     4441 ggtgcccatc cgcaaggaga tgccctggcc gcaggccgcc ctgatgggct gctgcgtgcc
     4501 caccggagtc ggcgcggtga cccggtgcgc ggaggtggag gcgggcgcct cggtgctcgt
     4561 catcggctgc ggcggcgtgg ggctcaacgt ggtgcagggc gccaggctgg ccggggccgg
     4621 catcatcgcg gccgccgacc tgctcgacag caagctcgcc tacgccaagg acttcggcgc
     4681 gacccacacc atcaacgcca agagcgagaa cgtggtggac cgcgtgcgcg agctcacccc
     4741 gggcggccgc ggcgtggact acgccttcga cgccatcggc ggcgaggcca ccactctgca
     4801 gatcgtggag gcgatccggc cgggcggcac cgcggtgatc gtgggcatgg ccgcgctcag
     4861 tgtccgcgcg cccatcacgc cctacatgat ggcgctccag gaaaaggcca tcaagggcac
     4921 catgtacggc tcggtgcgcc cgaacatcga cttccccaag ttcgtggatc tctatctcga
     4981 gggccgg
//
LOCUS       DASUFA010000359         9094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_897345, whole genome shotgun sequence.
ACCESSION   DASUFA010000359 DASUFA010000000
VERSION     DASUFA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897345"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFX87_13125"
     CDS             <1..635
                     /locus_tag="VFX87_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4995916.1"
                     /translation="VGYRRSAAQARAAVRELEAVGVRAAALRADVARPREARALVAGA
                     VRRLGRLDILVNNAALFFRTPVLTTTPAQFDRLLAVNLRAPFFVSQAAALAMGRRGGR
                     IINIADVGAVRAWAGYVPYGISKAGVVMLTRGLAVALAPRIQVNAVAPGVVLLPEGFP
                     RQSGRRLAARIPMGRHGRPADVAEAVRFFATCSDYITGQVLFVDGGMSAL"
     gene            complement(642..1532)
                     /locus_tag="VFX87_13130"
     CDS             complement(642..1532)
                     /locus_tag="VFX87_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX4995917.1"
                     /translation="MAAPALLIVNPAAGGGRARRLWPSLRDSLIRQHFAFEMVETRAR
                     GHAATLAAEAARARVRLVVAVGGDGTLNEVVNGLVPLRTEYPVTVGALMTGRGRDACR
                     NLGLARDPRHAARRLVEGRVVALDVGLARWPGGHRYFLGSAGTGFDAVVAERAGARGG
                     RLSYARAVLTSLRDYRPVEIAVRLDDADACAGPAASVVVCNGAWFGGGMRIAPGARPD
                     DGLLDVVRLGALGRLELARWLPTVYWGGHLANPRVATRRAVRVRVDAGAPLPVQLDGE
                     LGAHTPLDVGICPGALRLLV"
     gene            complement(1529..2215)
                     /locus_tag="VFX87_13135"
     CDS             complement(1529..2215)
                     /locus_tag="VFX87_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4995918.1"
                     /translation="MAESPLYLKQVELGPMQNFVYLIGDQASRECVVVDPAWEIDGIA
                     ELVARDGMRLIGALVTHTHQDHVGGHLFGHDIPGVEELLAKHPMKVYVHQDEREFLKG
                     FGSDVVQVRGGDTLQVGQVPLTFIHTPGHTPGSQCFLVDGCLISGDTLFIRSCGRTDL
                     PGSDPQEMYTSLTQRLGALPEETVVLPGHNYGGSQTTIGAEKRENPMMRFPSMSDFMR
                     AMGGGRVRRT"
     gene            complement(2215..2604)
                     /locus_tag="VFX87_13140"
     CDS             complement(2215..2604)
                     /locus_tag="VFX87_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995919.1"
                     /translation="MRTLTVIASLALLALGGPLGPATAGELRPTVLAPNPFAMTPPPG
                     PIEPRPAPSVPGRPSHGHPGRPPVFSAPSVVCCAGYWAYQWVPTAYTTYVWVPGYVTA
                     EGVMVGGGYQPQIVSGGYYQLVWVNGN"
     gene            complement(2672..4447)
                     /locus_tag="VFX87_13145"
     CDS             complement(2672..4447)
                     /locus_tag="VFX87_13145"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX4995920.1"
                     /translation="MARGKTLVVLLLLAAGLGGFFYYDTYWLNPKREKAESAKGRIWT
                     VEPKDVEGVTIARKGETIRLKRTADGWEMLEPLRARADRSAVDDMITGLATARMDREI
                     DPNPAKPADFGLAPPEAEVRLEVKGAAAPLVLRVGTKNPTGVWVYAREGDKPAVITMS
                     ESVARDTTRPVADFRDRSVMAFDRRNLTGLDLDVAGDQIGLVADEPGKWRIVKPRPLR
                     ADADIVADFLEKLEGAKALEFADDAPKSLAPYGLDKPSRVTVWLGRDKDRSSRTLLVG
                     RPVPEKKGVYVKRDGEPGVLLTAEAVWTTLPKTVAVLRDKTVVTYAYDKLTKVELAHA
                     RDGVTLEKDGSGWKLTAPEALKADSSAVTGLLWKIRDLRALGFLAETPAEAPRFLTRP
                     EVTVRLWEEGAKEPKTLLLQSSAERRGGQPVAVAAVRGEGPVMLVDGKMLADLAPGVA
                     ELRDRALFPAFEIGDIKKARVAAGDKPLVVEKSGETEWKVVEPSRGNTKEGRVAGMLL
                     TLRGLKWKEIAAKGPEDAAKWGLDKPEVELTLFKDGGAELATLLVGRTDGAVTYVKLK
                     ADPAIFAVSSKDLDDLRKARAEIPS"
     gene            complement(4452..6029)
                     /locus_tag="VFX87_13150"
     CDS             complement(4452..6029)
                     /locus_tag="VFX87_13150"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX4995921.1"
                     /translation="MNWLKRHTLTAAVVSLLVAFALWHFYPGVPAQWVFLGLGGALLL
                     LSLAFNAGEARAAMGTRTARYGAGAAVMALLALGIVVAANAISLRHSVRWDFTENKRN
                     SVSPQTIQVLRTLKAPVSAIAFFRSDTPGKKTAEDLLTQYATYSRGKFTWRLEDTDRA
                     PALARQYGVESYGTVVLEGGPAGQTRTEKVQDAEEEKLTNAIVKITRAGKRVVYVLKG
                     HGEREIGSTDRAGFSQAKEQMEKANYEVKDLFLARDPKIPEDAAIVMVPSPKTDLFPQ
                     ELAAIDGYISRAGKVLFMAAPFQAETTAKYLTKYGIVLDEDVVIELNPIGQLFGVGPL
                     VPVVGQYEAHPITKDMGGVMTLFPLTRSVAPAKALPKGVQASPLAQTSRQSWGETDKN
                     VFQTGKATPDPTEKTGPLSVALVATVDAQPDPKAEGDAAKKAPKARIVVVGTADFASN
                     QFLGAQGNRDFFLNIVSWLAEEEDLISIRAKDPKQNPVVLTSGQSRLVLGLPILVLPG
                     AVLICGIAVIMQRRRAR"
     gene            complement(6034..6801)
                     /locus_tag="VFX87_13155"
     CDS             complement(6034..6801)
                     /locus_tag="VFX87_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4995922.1"
                     /translation="MRWLPVFKKEMRLYFGSPVAYVVFTFFLVISGWFFSQIFLFYSD
                     ASMRSFMQPQFGQNLNVVDNVMRPLFTNMSVVLLFFIPMLTMRLFAEEKKSGTMELLL
                     TYPVRDGEVLAGKYLAALALYLILLGLTLLYPGLVAYFTRVEWGPILTGYLGLILTGA
                     VFLAVGVLVSSLTENQIVAGFGTFGMLLAFWVIGWGAEFAGGNLRTVLQYLSIGDHLE
                     GFTRGLIDTKDLVYYLTGVALALFLTLRSLESKRWRG"
     gene            complement(6801..7778)
                     /locus_tag="VFX87_13160"
     CDS             complement(6801..7778)
                     /locus_tag="VFX87_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4995923.1"
                     /translation="MIQVDHLTKRYGPVTAIHDVSFNVDKGRIVGFLGPNGAGKSTTM
                     KILSCFMPASGGTARVAGYDVFSQSLEVRRRIGYLPENAPLYPDLSVASYLDFVAEIK
                     GVGRAARRGRVADVMERCFIGDMQNRLIGKLSKGYRQRVGLAQALLGDPEVLILDEPT
                     IGLDPRQIAEIRALIRSLAGQHTVILSTHILPEVSMVCDGVVIINRGRIVAQGTESEL
                     VQQAFPSARIELRVAGADADVAGAVAAIPGVVGVQPLAARDGGVGVLVEAARDRDVRP
                     DLVRLVTGRGWTLQELHPVGMSLEEVFIRVVAGEQEPQELVPAAVEETR"
     gene            complement(7827..>9094)
                     /locus_tag="VFX87_13165"
     CDS             complement(7827..>9094)
                     /locus_tag="VFX87_13165"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX4995924.1"
                     /translation="ANNLLSALIDNHVYFGNPLGLDPRRVIWRRCMDMNDRSLRRTVT
                     GLGGPKEGVPRETGFDITAASEIMAILCLSADADDLRARIDRILVGFTYDGRPVTAKE
                     LGGTGAMVALLADAMKPNLVQSIEGVPAFIHGGPFANIAHGCNSVLATRLALKLGDIC
                     LTEAGFATDLGAEKFFDIKCRLAGLKPDAAMIVVTARALKYHGGVPVPELDRENVAAL
                     TRGLDNLEAHVEAVRQFKVPVLVGMNRYPSDTEAEYAAVRERCARLGVAVHVADVFGR
                     GGAGGEDLARGLLALLARERSAFAPLYALDDPITVKLDTIARRIYGADGVEYPKRVER
                     QIAQAESLGYGRLPICVAKTQRSLSDDPTLLNRPRGFKITVNEVRISAGAGFLVAITG
                     DITTMPGLPRRPNAERVDVTPDGVITGLF"
BASE COUNT         1335 a         3287 c         3151 g         1321 t
ORIGIN      
        1 cggtgggcta ccgccgctcg gccgcgcagg cgcgcgccgc ggtgcgcgag ctcgaggcag
       61 tcggcgtgcg cgcggccgcg ctgcgcgccg acgtggcccg gccgcgcgag gcacgtgcgc
      121 tcgtggcggg cgcggtgagg cggctcggcc ggctcgacat cctggtcaac aacgcggcac
      181 tgttcttccg cacgccggtg ctcaccacca cccccgccca gttcgaccgg ctcctcgccg
      241 tcaacctgcg cgcgccgttc ttcgtgagcc aggcggccgc cctcgccatg ggccggcgcg
      301 gcgggcgcat catcaacatc gcggacgtcg gtgcggtccg cgcctgggcc ggctacgtgc
      361 cctacggcat ctccaaggcg ggcgtggtca tgctcacccg cggcctcgcc gtggccctgg
      421 ccccgcgcat ccaggtgaac gcggtggcgc ccggcgtggt gctgctgccc gagggcttcc
      481 cccgccaatc ggggcgccgc ctcgccgcac gcatcccgat ggggcggcac gggcggcccg
      541 ccgacgtggc ggaggcggtc cgcttcttcg ccacctgctc cgactacatc accggccagg
      601 tgctgttcgt ggacgggggc atgtccgccc tctagcgcgc gctacacgag caggcggagg
      661 gcgccggggc agatcccgac gtcgagcggg gtgtgcgcgc cgagctcgcc gtcgagctgg
      721 accggaagcg gcgcgcccgc atccacccgc acccggaccg cgcgccgggt cgcgacccgg
      781 ggattggcga gatggccgcc ccagtacacc gtgggcagcc agcgcgccag ctcgagccgt
      841 cccagcgcgc ccaggcgtac cacgtcgagg aggccgtcat cggggcgcgc gcccggcgcg
      901 atgcgcatgc ctccgccgaa ccaggcgccg ttgcatacca ccacgctcgc cgcgggaccg
      961 gcgcaggcgt ctgcatcgtc cagacgcacc gcgatctcga cgggccggta gtcgcgcagg
     1021 ctcgtcagca ccgcgcgcgc atagctcagc cgaccgccgc gcgccccggc ccgctcggcc
     1081 accaccgcgt cgaatccggt gccggccgag cccaggaagt agcggtggcc gcccggccac
     1141 cgggcgagcc cgacatcgag cgcgacgacg cgcccctcga ccaggcggcg cgccgcgtgc
     1201 cggggatccc gcgccagccc caggttgcgg caggcatcgc gcccgcgtcc ggtcatgagc
     1261 gcgccgacgg tcaccggata ctccgtgcgc agcggaacga ggccgttgac cacctcgttc
     1321 agcgtgccgt cgccgcccac cgcgaccacg aggcggacac gcgcccgcgc ggcctcggcg
     1381 gccagcgtcg cggcgtggcc gcgcgcccgc gtctcgacca tctcgaaggc gaagtgctgc
     1441 cggatgagcg agtcgcggag ggagggccag aggcgccgag cgcggccgcc gccggccgcg
     1501 gggttgacga tgaggagggc gggagcggct atgtgcggcg gacgcgcccg ccgcccatgg
     1561 cgcgcatgaa gtcgctcatg gacggaaacc gcatcatcgg gttctcacgc ttctcggcgc
     1621 cgatggtggt ctgcgacccg ccgtagttgt gaccgggcag caccaccgtc tcctcgggca
     1681 gcgcacccaa gcgctgcgtg agcgaggtgt acatctcctg cggatcgcta ccgggcagat
     1741 cggtgcgccc gcacgagcga atgaagagcg tgtcgccgga gatgaggcag ccgtccacca
     1801 ggaagcactg cgagcccggt gtgtgcccgg gcgtatggat gaaggtcagc ggcacctgcc
     1861 ctacctggag cgtgtccccg ccccgcacct gcaccacgtc cgagccgaag cccttgagga
     1921 actcccgctc gtcctggtgc acgtagacct tcatggggtg cttggcgagc agctcctcca
     1981 cgccggggat gtcgtggccg aagagatggc cgcccacgtg gtcctgatgc gtgtgggtga
     2041 ccagcgcgcc gatgagccgc atgccgtccc tggccaccag ctctgcgatg ccgtcgatct
     2101 cccaggccgg atccaccacg acacactccc ggctcgcctg gtcgccgatg aggtagacga
     2161 agttctgcat cggccccagc tcgacctgct tcagatagag cggcgactcc gccatcagtt
     2221 tccgttcacc cacaccagct ggtagtagcc gccgctcacg atctgcggct ggtatccgcc
     2281 cccgaccatg actccctccg ccgtgacgta ccctggcacc cacacgtacg tcgtgtacgc
     2341 ggtgggcacc cactggtagg cccagtagcc cgcgcagcag accaccgacg gggccgagaa
     2401 gaccggcggg cggcccgggt gcccgtggga cggccgcccc ggcacgctgg gcgcggggcg
     2461 gggctcgatc ggcccgggag gcggcgtcat ggcgaaggga ttgggggcca gcacggtcgg
     2521 ccgcagctca ccggcggtcg ccggaccgag cggcccgccc agggcgagga gcgcaagact
     2581 tgcgatgacg gtgagtgtcc tcatgctcga tcggacgcct atctcgacgg ccctattctg
     2641 ccaccgtcgc cccggccgga gaagccgggt ttcaggaagg gatctcggct cttgccttcc
     2701 gcaggtcgtc caggtccttg ctggacaccg cgaagatggc ggggtcggcc ttcaacttca
     2761 cgtaggtgac cgcgccgtcg gtacgcccga ccaggagcgt ggccagctcg gcgccgccgt
     2821 ccttgaacag ggtcagctcc acctcgggct tgtcgaggcc ccacttggcc gcgtcctccg
     2881 ggcccttggc cgcgatctcc ttccacttga ggccccggag cgtcaggagc atgcccgcca
     2941 cgcgaccttc cttggtgttc ccgcgcgacg gctccacgac cttccactcc gtctcgccgc
     3001 tcttctccac gaccaggggc ttgtcgccgg cggccacgcg cgccttcttg atgtcgccga
     3061 tctcgaacgc ggggaacagg gctcggtcgc gcagctcggc gaccccgggc gcgaggtccg
     3121 cgagcatctt cccgtcgacc agcatcaccg gcccctctcc ccgcaccgcg gccaccgcca
     3181 cgggctgccc gccgcggcgc tccgccgagg actgcagcag gagcgtcttc ggctccttgg
     3241 cgccctcctc ccagagccgc accgtgacct cggggcgggt caggaagcgc ggggcctccg
     3301 ccggcgtctc ggcgaggaag ccgagggcgc gcaggtcgcg gatcttccac agaagcccgg
     3361 tcaccgcgga cgaatcggcc ttgagcgcct ccggcgcggt gagcttccag ccggagccgt
     3421 ccttctccag cgtgacgccg tcgcgcgcgt gcgcgagctc gaccttcgtg agcttgtcgt
     3481 aggcgtaggt gaccacggtc ttgtcgcgca gcaccgccac cgtcttcggc agcgtcgtcc
     3541 acactgcctc cgcggtcagg agcacgcccg gctcgccgtc gcgcttgacg tagaccccct
     3601 tcttctccgg caccgggcgc cccacgagca gggtccggga cgagcggtcc ttgtcacggc
     3661 ccaaccacac cgtgacgcgg gacggcttgt cgagcccgta gggcgcgagc gacttcggcg
     3721 cgtcgtccgc gaactcgagc gccttggcgc cctccagctt ctcgaggaaa tcggccacga
     3781 tgtccgcgtc ggcgcggagc gggcgcggct tcacgatgcg ccacttgccc ggctcgtcgg
     3841 cgacgaggcc gatctgatcg ccggccacgt ccaggtcgag gccggtgaga ttgcgccggt
     3901 cgaaggccat cacgctgcga tcgcggaaat ccgccaccgg tcgcgtggtg tcgcgcgcga
     3961 cgctctcgga catcgtgatc accgcgggct tgtcgccctc gcgcgcgtag acccagaccc
     4021 cggtcgggtt cttggtgccc acccggagga cgagcggcgc ggcggcgccc ttgacctcga
     4081 ggcgcacttc cgcctccggg ggcgcgagcc cgaagtcggc cggcttggcg ggattgggat
     4141 cgatctcccg gtccatccgc gcggtggcca ggccggtgat catgtcatcg accgcggatc
     4201 ggtcggcccg cgctcgcagg ggctcgagca tctcccagcc gtccgcggtg cgcttcaggc
     4261 ggatggtctc gcccttgcgg gcgatggtga cgccctcgac gtccttgggc tcgacggtcc
     4321 agatgcgccc cttcgcggac tccgccttct cgcgcttggg gttgagccag taggtgtcgt
     4381 agtagaagaa gccgccgagg ccggcggcga gcagcagcag gacgacgagg gtcttgcctc
     4441 gcgccatggg gctagcgcgc gcggcgccgc tgcatgatca ccgcgatgcc gcagatcaag
     4501 acggcgcccg gcagcacgag gatgggcagg cccagcacga gccgggactg cccggaggtc
     4561 agcaccaccg ggttctgctt cggatccttg gcgcgaatcg agatgagatc ctcctcttcc
     4621 gcgagccagg acacgatgtt gaggaagaag tctcggttgc cctgcgcccc caggaactgg
     4681 ttggaggcga agtcggcggt gcccaccacg acgatgcggg ccttgggcgc cttcttcgcc
     4741 gcgtcgccct ccgctttcgg atcgggctgg gcgtccaccg tggccaccag cgccaccgag
     4801 agcggccccg tcttctcggt cggatccggc gtcgccttgc cggtctggaa cacgttcttg
     4861 tcggtctcgc cccacgactg gcggctggtc tgggccagcg gcgaggcctg cacgcccttc
     4921 ggcagcgcct tggccggcgc caccgagcgc gtgagcggga acagcgtcat gaccccgccc
     4981 atgtccttgg tgatcggatg cgcctcgtac tggcccacca ccgggacgag cggccccacc
     5041 ccgaagagct ggccgatcgg attcagctcg atcaccacgt cctcgtccag cacgatcccg
     5101 tacttggtca ggtacttcgc ggtggtctcg gcctggaagg gcgcggccat gaagaggacc
     5161 ttccccgccc tggagatgta cccgtcgatc gcggccagct cctgcgggaa gagatccgtc
     5221 ttcgggctcg gcaccatcac gatggccgcg tcctcgggga tcttcgggtc ccgggccagg
     5281 aacagatcct tgacctcgta gttggccttc tccatctgct ccttggcctg gctgaagccg
     5341 gcccggtccg tgctcccgat ctcgcgctcg ccgtggccct tgagcacgta gacgacccgc
     5401 ttgcccgcgc gggtgatctt gacgatggcg ttggtgagct tctcctcctc ggcgtcctgc
     5461 accttctcgg tacgcgtctg cccggctggg ccgccctcca gcaccacggt gccgtagctc
     5521 tccaccccgt actgccgggc cagggcgggc gcccggtccg tgtcctccag acgccacgtg
     5581 aacttgccgc gcgagtaggt cgcgtactgg gtgaggagat cctccgccgt cttcttgccc
     5641 ggcgtgtccg agcggaagaa ggcgatcgcg ctgaccgggg ccttcagggt ccggagcacc
     5701 tggatcgtct ggggcgagac gctgttgcgc ttgttctcgg tgaagtccca gcgaacgctg
     5761 tgccgcagcg agatcgcgtt ggccgcgacc acgatgccca gggcgagcag cgccatcacc
     5821 gcggcccccg cgccgtagcg cgcggtgcgg gtgcccatcg cggcgcgcgc ctcgcccgca
     5881 ttgaaggcca gcgagaggag cagcagggcg ccacccaggc cgaggaagac ccactgggcc
     5941 ggcacgcccg gatagaagtg ccagagagcg aacgccacca gcagggagac caccgccgcg
     6001 gtcagcgtgt ggcgcttgag ccagttcatc gggctagccc ctccaccgct tggactcgag
     6061 cgagcgcagg gtgaggaaca gggccagcgc cacgccggtc aggtagtaca cgaggtcctt
     6121 ggtgtcgatg aggccgcggg tgaagccctc gaggtggtcg ccgatcgaga gatactggag
     6181 cacggtgcgc aggttgccgc ccgcgaactc ggcgccccag ccgatgaccc agaacgcgag
     6241 cagcatcccg aaggtgccga agccggcgac gatctggttc tcggtgagag aggagacgag
     6301 cacgcccacc gcgaggaaga ccgcgccggt caggatcagc cccaggtagc cggtcaggat
     6361 cggcccccac tcgacccggg tgaagtacgc gaccaggccc gggtagagca gggtcaggcc
     6421 gagcaggatc aggtagaggg cgagggcggc caggtacttg ccggcgagca cctccccgtc
     6481 gcgcaccggg taggtcagca gcagctccat ggttcccgac ttcttctcct cggcgaagag
     6541 ccgcatggtc agcatcggga tgaagaacag gagcaccacg ctcatgttgg tgaagagcgg
     6601 gcgcatcacg ttgtccacca cgttgagatt ctggccgaac tgcggctgca tgaacgaccg
     6661 catggaggcg tcgctgtaga agaggaagat ctggctgaag aaccagccgg agatcaccag
     6721 gaagaacgtg aagaccacgt aggccaccgg ggagccgaag tagagccgca tctccttctt
     6781 gaacacgggc agccagcgca tcagcgcgtc tcctccaccg ctgccgggac cagctcctgc
     6841 ggctcctgct cccccgcgac cacgcgaatg aagacctcct cgagactcat cccgaccggg
     6901 tgcagctcct ggagcgtcca gccgcggccg gtgacaaggc ggaccaggtc gggccgcacg
     6961 tcgcggtcgc gcgccgcctc caccagcacc ccgaccccgc cgtcgcgggc cgcgaggggc
     7021 tgcaccccca ccacacccgg gatcgcggcc accgcgccgg ccacgtcggc gtcggcgcca
     7081 gccacccgca gctcgatgcg cgccgacggg aaggcctgct ggacgagctc cgactcggtg
     7141 ccctgggcta cgatgcgccc gcggttgatg atgacgacgc cgtcgcacac catcgagacc
     7201 tcgggcagga tgtgggtgga caggatcacg gtgtgctgcc cggccagcga ccggatcagc
     7261 gcgcggatct ccgcgatctg ccgcgggtcg agcccgatgg tgggctcgtc caggatcagt
     7321 acctccggat cgcccaggag ggcctgggcc agccccaccc gctgccggta gcctttcgag
     7381 agcttgccga tcagccgatt ctgcatgtcc ccgatgaagc accgctccat cacgtcggcc
     7441 acccgaccgc gccgcgcggc gcgccccacg cccttgatct cggccacgaa gtcgagatag
     7501 gaggcgaccg agagatccgg gtagagcggc gcgttctccg ggagatagcc gatccgccgg
     7561 cggacctcca gcgactgcga gaacacgtcg tagccggcca cccgcgcggt gccgccgctg
     7621 gcgggcatga agcacgagag gatcttcatc gtggtggact tgcccgcccc gttggggccg
     7681 agaaatccca cgatgcggcc cttgtccacg ttgaacgaca catcgtggat ggccgtgacc
     7741 ggtccgtaac gctttgtcag atggtcgacc tggatcatac gtgcggcccg aattccgcgg
     7801 gagccgccgc aaagtatagc atagggctag aagagccccg tgatgacgcc gtccggcgtg
     7861 acgtccactc gctccgcgtt gggacggcgc ggcagcccgg gcatggtggt gatgtcaccg
     7921 gtgatggcca ccaggaagcc agcgcccgcg gagatgcgca cctcgttgac cgtgatcttg
     7981 aacccgcggg ggcggttcag cagggtcgga tcgtcggaca gcgagcgttg agtcttcgcg
     8041 acgcagatcg ggagcctccc gtagccgagt gattcagcct gcgcgatctg acgctcgacc
     8101 cgcttgggat attcgacgcc gtcagcgccg tagatccgcc gggcgatcgt gtcgagcttc
     8161 accgtgatgg ggtcgtcgag cgcgtagagc ggcgcgaagg ccgagcgctc cctggccagg
     8221 agggcaagca aaccccgggc cagatcctcg ccgcccgcgc ccccgcgccc gaacacgtcg
     8281 gccacgtgca ccgccacgcc cagccgggcg cagcgctccc ggaccgccgc gtactccgcc
     8341 tcggtgtcgg acgggtaccg gttcatcccg acgagcaccg gcaccttgaa ctggcgcacc
     8401 gcctccacgt gcgcctccag gttgtcgagc ccgcgggtga gcgcggccac gttctcccgg
     8461 tcgagctcgg gcaccggcac gccgccgtga tacttcagcg cccgcgcggt caccacgatc
     8521 atggccgcgt cgggcttgag cccggcgagc cggcacttga tgtcgaagaa cttctcggcg
     8581 cccaggtcgg tggcgaagcc cgcctcggtg aggcagatgt cgccgagctt gagcgccagc
     8641 cgggtggcca gcaccgagtt gcagccgtgg gcgatgttgg cgaacggacc gccgtggatg
     8701 aacgccggca cgccctcgat cgactgcacc aggttcggct tcatcgcgtc ggccaggagc
     8761 gccaccatcg cgcccgtgcc gccgagctcc ttggcggtca ccggccggcc gtcgtaggtg
     8821 aagccgacca ggatgcggtc gatgcgcgcg cgcaggtcgt cggcgtcggc cgagaggcag
     8881 agaatcgcca tgatctcgga agcggcggtg atgtcgaagc cggtctcccg cggcacgccc
     8941 tccttcggcc cgccgagacc ggtcacggtc cgccgcagcg agcggtcgtt catgtccatg
     9001 cagcgccgcc agatcacccg gcgcgggtcg aggccgagcg ggttgccgaa gtagacgtga
     9061 ttgtcgatca gcgccgagag gaggttattg gcgg
//
LOCUS       DASUFA010000360         9483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_768704, whole genome shotgun sequence.
ACCESSION   DASUFA010000360 DASUFA010000000
VERSION     DASUFA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768704"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2322
                     /locus_tag="VFX87_13170"
     CDS             190..2322
                     /locus_tag="VFX87_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX4995925.1"
                     /translation="MLDHRIELDPLLKPRSVAVLGASERPSVGRTIVENLDRIGFDGE
                     IHPINPRYETLLGRRCYRSVEELPAGVDVLAFCVNHERVLDGLRAAARKGVRAAVAFD
                     AGFAESDDAGRRRQDELVAICREAGIAFCGPNCMGVISPHERSLVYLNALRDPAPLAG
                     NVGLISQSGSIVIGLLADCRRFGWSRVISTGNEAVIVAADVLEYLVDDPATRVIAMFL
                     ESVRRPERFVAALDRAADQGKPVVVLKVGRSERARRAITSHTGGLAGEARVVEAVLRG
                     HRAIEVHDLDELTETLAACQGARRPAGRRIAVVTNSGGHTELILDVAASVGLELPPLS
                     PATRAEAARVIGPIAGDGNPLDAWGNGDFQTNVPHALRTLGADPGIDAVVWCTDSADA
                     SPMGTPERFMTYAGMLVEGAKGSTKPFYLMGTRPGIFRRDQHELLSAQGIALIGGTRQ
                     GLRAIDRLARQAAPLAPLRLPSGTSMPTLATIPGGTARRTIHEHDAKRLLAEAGLPIV
                     KETLCSSCEEATAAAGVIGYPVALKLVADDVPHRSDLGLVAVGLRDARELGEEWDRMA
                     AVRAGKLGDIAVAGFVVQQMVSGGVEVLAGVSHDRDFGPVLAFGLGGVAAEALARLAL
                     RPLPLRVGDAEAMIAEVPAAATLLGGFRGQPPADVDSLHRCLEALADYAWADRAAVAE
                     IDLNPIKVLPRGRGCVVVDALIVPRAPR"
     gene            2333..3148
                     /locus_tag="VFX87_13175"
     CDS             2333..3148
                     /locus_tag="VFX87_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4995926.1"
                     /translation="MAEEIVTYTVDGRIGIVTINRPDKLNAISHELKHALVERLRAAD
                     ADPGTSVVVLRAEGRSFSAGYDISPNPARAARRGNALAWHESLTDDIRLEMTPWDMSK
                     PVIASVQGHCLGGGCELMMLCDLAIASDDAVFGEPEIRFSNVGPGLVMPFVIGLRRAR
                     ELLYLGDPIDAQTALAYGMVNRVVPRAELQAATLKFARRMALISPESLVATKLATRRG
                     AEAAGLRNAILAGLDVLAPLYAARTEVGMKFDEIREKEGLGAALKWRAAQFAD"
     gene            complement(3172..4746)
                     /locus_tag="VFX87_13180"
     CDS             complement(3172..4746)
                     /locus_tag="VFX87_13180"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4995927.1"
                     /translation="MPARSGAEYIESLKKQPPRVFLGGRRITDVTAEPVFQEPIRAIA
                     EQYDMQLDPAYGDVMTYPSPTTGDPVSTSFLVPYSREELVKKRKHFKLRADHNFGFMG
                     RSPDFMNQFVTGWHLMADRFARAGARFGENATRYYEYVRERDLFLTHMLINPQIDRSK
                     TSAQQEDPYLHLGRVRETPDGIVVRGAKMLGTMAPITEEVAVIPFGGVAPGDDAYALA
                     FGIPSSTPGLTFICRETVSPLPRSRFDHPLSSRFEEMDCIAVFEDVLVPWDRVMVDGG
                     PGSAEVINTMGADYGALLNVQTSARMLSQLEFFCGLGMKVADAIGITGFLHIQEKLGE
                     MLSQMEIARAVFYGSEAMAQKLPSGVWVPGGHGLRAFHLQTGKVYSRFVEIIQTLAAG
                     GFFYAPSEADLQSPEVRPHIDRYVRGRAGVSAEERIALFKLAWDATGEAFAQRMAQYV
                     RFYSGDPIRLTAGFYAQYDKAPLFELVERALGHREGVPLPITPDDPGAPIPYQPGTRG
                     MAGTYAATSLPSQPSS"
     gene            4843..5346
                     /locus_tag="VFX87_13185"
     CDS             4843..5346
                     /locus_tag="VFX87_13185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX4995928.1"
                     /translation="MDANVKKTALRMIPYGLYVLTAKGKDDTVAAATVNWVTQASFTP
                     PLVVVGVKTDSHAHALIKESKAFALNVLGKGQQAMAFTFFKPAAREGQTVSGAPFRWG
                     STGAPILEHAPAFVECRLVDTVERGDHSVFVGEVVEAGLGKPVEGRADDATLWLKELG
                     PNVFYGG"
     gene            complement(5440..6576)
                     /locus_tag="VFX87_13190"
     CDS             complement(5440..6576)
                     /locus_tag="VFX87_13190"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX4995929.1"
                     /translation="MHYVDPSTGQTYPLSEARWRADNGHYLNLAPGPGLRPRDIDTAR
                     RSVWRYARGLLVGAEHAVSLGEGWTPLLPGEWHGAPVHYKLEFMMPTGSFKDRGMTVM
                     VSYLKSRGVTHVLEDSSGNAGASLSAYAAAAGLTCRILVPETASYPKIAQIAACGADV
                     VTIRGSRQDVAEAALRQSADIFYASHNWQPFFVEGTKTLAYELWEQAGFRAPDNVVVP
                     LGYGSNVLGAERGFDELLRAGEIARRPRLFGVQAARCAPYAAAFRAGVEHLVAAEIAP
                     TVAEGIATPRPTRVADVLRAVRDTGGSIVAVEEAEIVHALRDLARRGLYVEPTSAVAA
                     AGLSRLLTAGVIRPQEDTVLVLTGSGLKASATIGDLLKLAPRPA"
     gene            complement(6589..7830)
                     /locus_tag="VFX87_13195"
     CDS             complement(6589..7830)
                     /locus_tag="VFX87_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4995930.1"
                     /translation="MADAVPAELRARVLEQIDDDEVVRLACELIRIPSFTTEETPLAE
                     WLASYLADQGLPVTLQEVEPGRKQTIARLRGTGGGRSIMLNGHIDIDPLASGWTRDPF
                     DPWIADGKLFGHGVYNMKAGVTACIMATLALQRARVPIRGDVVLACVAAELNSGIGTI
                     HALENGQMTDMAVVAEPYGARTILTKHTGTMDVVIHTLGRATHISKKEHGVDAIAMMM
                     RAVEALHAMRFTHTHDPDLPGLPRLNVGSIIGGRGRDHNLRGAYTVSDFCTAYVNVRF
                     NASQTGQTVVADIRQTLDALAAADPKFEFEIEFPPRPERGLGRLAKAAVDVPKTSPIV
                     QMVRRNLHLMTGAEPEHLGVRLPQSYAGNDTTHLWAAGIPCCLYGPGGGYTDYHDVHV
                     DLADLFLCTRVLALTALEATH"
     gene            7929..9173
                     /locus_tag="VFX87_13200"
     CDS             7929..9173
                     /locus_tag="VFX87_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017273458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX4995931.1"
                     /translation="MSASDSITIDAPRFWSTVMRSGEIGPGKSGGLRRLALTDADKEM
                     RDLFITWCTEAGCTVAVDRVGNIFARRPGVDDRLPPVLMGSHLDTQFAGGKYDGIVGV
                     LAGLEVLRTLDDRRVRTKRPLELVCWTNEEGARFTPPMVASGAFAGVFDVDWVLALRD
                     DDGKQFGGELERIGYNGKAAVGGRSIDAYFELHIEQGPILDREAIPVGIVVGGYAAHG
                     MHVDVHGETAHSGPTPMDRRRNALVGAAMLAVAVNEIGWKYHATEGKATVARLVAWPN
                     KAGILAEYAQVTCDVRHADRAVADQMWAEVKAAMAECAGRANVEMAIAGEWHFGSERF
                     DPGCIRLIRDAARERGVAHRDILSQAGHDAYYISRIAPTAMIFTPCRDGISHNEAEHA
                     ELDYTSPGVNVLLDAVLARANR"
     gene            9269..>9483
                     /locus_tag="VFX87_13205"
     CDS             9269..>9483
                     /locus_tag="VFX87_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995932.1"
                     /translation="MLGVAVGWQLYELTGSALDLGLVGLVQFLPMILLTLVVGQVADR
                     CDRRLIVIACEIVKAVAAAALALGALGG"
BASE COUNT         1323 a         3255 c         3508 g         1397 t
ORIGIN      
        1 gaacgatctg tacgccttcg accccaccgt gaaggtggag ctcgaggagt tcacggacgt
       61 ggtggggcgt cacttccgcc agccgaagga agggctcggc ttcgatcagg gcccggcggc
      121 gcacatgtgg cgcaccatca actggccgac gcgctacctg tagtcgcata gcggagcggt
      181 cgagcgatct tgcttgacca tcggatcgaa ctcgacccgc tcctgaaacc ccgctcggtc
      241 gccgtgctcg gagcgtccga gcgcccctcg gtgggccgca cgatcgtgga gaacctcgat
      301 cgtatcggat tcgacgggga gatccatccc atcaaccccc ggtacgagac cctgctcggc
      361 cggcgctgct accgatcggt cgaggagctc cccgccggcg tcgacgtgct cgcgttctgc
      421 gtcaaccacg agcgggtgct cgatggcctc cgggccgccg cccgaaaggg agtccgcgcc
      481 gcggtcgcgt tcgacgcggg gtttgccgag agcgacgacg cgggccgccg ccggcaggac
      541 gagctggtcg ccatctgtcg cgaggcgggg atcgcgttct gcgggccgaa ctgcatgggc
      601 gtcatcagcc cgcacgagcg gagcctcgtc tacctgaacg cgctccgcga cccggccccg
      661 ctcgcgggca acgtcgggct gatctcccag agcgggtcga tcgtcatcgg gctgctcgcc
      721 gactgccggc gattcggctg gagccgcgtg atctcgaccg ggaacgaggc ggtcatcgtg
      781 gccgccgacg tcctcgagta cctggtcgac gacccggcca cgcgcgtgat cgcgatgttc
      841 ctcgagagcg tgcggcggcc ggagcggttc gtcgccgcgc tcgaccgcgc cgccgatcag
      901 ggcaagccgg tggtggtcct caaggtcggc cgcagcgagc gggcgcgccg ggcgatcacg
      961 agccacaccg gcggcctcgc cggcgaggcg cgcgtggtcg aagcggtcct ccgcgggcat
     1021 cgggcgatcg aggttcacga cctggacgag ctgaccgaga cgctcgccgc ctgccagggt
     1081 gcgcgccggc ccgcgggtcg tcggatcgcg gtggtgacga actccggcgg ccacaccgag
     1141 ctgatcctcg acgtcgccgc gagcgtcggc ctcgagctgc cgccgctctc gcccgccacg
     1201 cgcgccgagg ccgcgcgcgt catcggcccc atcgcgggcg acggcaaccc gctcgacgcg
     1261 tggggcaacg gcgactttca gaccaacgtg ccgcacgcgc tccggacgct cggggcggat
     1321 ccggggatcg acgcggtggt ctggtgcacg gacagtgccg acgcctcccc gatgggaacg
     1381 cccgagcgct tcatgacgta cgccgggatg ctggtcgagg gcgccaaggg atcgaccaag
     1441 cccttctatc tgatgggcac gcggccgggc atctttcggc gcgaccagca cgagctcctg
     1501 agcgcccagg ggatcgcgct gatcggcggc acgcgccagg ggctccgcgc gatcgatcgc
     1561 ctggcgcgac aggctgcgcc actcgcgccg ctccgtctcc catcggggac gagcatgccg
     1621 acgctggcga cgattccggg tggcacggcg cgccggacga ttcacgagca cgacgccaag
     1681 cggctgctcg ccgaggccgg gttgcctatc gtgaaggaga cgctctgctc gagctgcgag
     1741 gaggcgaccg cggcggcggg cgtgatcggg tacccggtgg cgctcaagct cgtggccgat
     1801 gacgtgcctc accggtcgga tctcggcctg gtggccgtcg ggctcaggga cgctcgtgag
     1861 ctcggcgagg agtgggaccg catggccgcg gtccgcgcgg ggaagctggg ggacatcgcg
     1921 gtcgccggct tcgtggtcca gcagatggtg agcgggggcg tcgaggtcct cgccggggtc
     1981 agccacgatc gggacttcgg ccccgtgctc gcgttcgggc tcgggggtgt cgccgccgag
     2041 gcgctcgccc gcctcgcgct ccggccgctg ccgctccgtg tcggcgacgc cgaggcgatg
     2101 atcgccgagg ttcccgccgc cgccacgctg ctgggcggct tccgcggcca accccccgcc
     2161 gacgtcgaca gcctccaccg gtgtctcgaa gcgctcgccg actacgcgtg ggccgaccgg
     2221 gccgccgtcg ccgagatcga tctgaaccca atcaaggtgc tgccccgggg ccggggctgc
     2281 gtcgtcgtcg acgcgctcat cgtcccgcgc gcgccgcgct gaggagtcgc caatggccga
     2341 ggagatcgtg acctacaccg tcgacggccg catcggcatc gtgacgatca accggcccga
     2401 caagctcaac gcgatcagcc acgagctcaa gcacgcgctg gtggagcgcc tccgcgcggc
     2461 ggacgccgac ccgggcacga gcgtggtggt cctgcgggcc gagggccgga gcttcagcgc
     2521 cggctacgac atctcgccca atccggcccg cgccgctcgg cggggcaatg cgctggcgtg
     2581 gcatgagtcg ctcaccgacg acatccggct cgagatgacg ccgtgggaca tgagcaagcc
     2641 ggtgatcgcg tcggtccagg gccactgcct gggcggcggc tgcgagctca tgatgctttg
     2701 cgatctcgcc atcgcctccg acgacgcggt cttcggcgag cccgagatcc gcttctcgaa
     2761 cgtcggcccc ggcctcgtga tgcccttcgt gatcggcctg cggcgcgcgc gcgagctcct
     2821 ctacctcggt gaccccatcg atgcgcagac cgccctcgcg tacgggatgg tcaaccgcgt
     2881 cgtcccccgc gccgagctcc aggccgcgac cctgaagttc gcccgacgga tggcgctgat
     2941 ctcaccggag tcgctcgtgg ccaccaagct cgcgacccgg cgcggtgccg aggcggcggg
     3001 ccttcgcaat gcgatcctgg ccgggctcga cgtgctcgct ccgctctacg cagcgcggac
     3061 cgaggtcggg atgaagttcg acgagatccg ggagaaggag ggtctggggg cggcgctgaa
     3121 gtggcgcgcg gcccagttcg ccgactagct gctcggaaga acagcgtcgg actacgacga
     3181 gggctgggac gggagcgacg tcgccgcgta cgtcccggcc atgccgcgcg tcccgggctg
     3241 gtaggggatc ggcgcgccgg ggtcgtcggg cgtgatcggc agcggcacgc cctcgcggtg
     3301 gccgagcgcg cgctcgacca gctcgaagag cggcgccttg tcgtactggg cgtagaagcc
     3361 ggcggtgagc cggatcgggt cgccggagta gaagcgcacg tactgcgcca tgcgctgggc
     3421 gaaggcctcg ccggtcgcgt cccaggcgag cttgaagagc gcgatgcgct cctcggcgga
     3481 gaccccggcg cggccgcgca cgtagcggtc gatgtggggc cgcacctccg ggctctgcag
     3541 atccgcctcg ctcggggcgt agaagaagcc gcccgcggcc agcgtctgga tgatctcgac
     3601 gaagcggctg tagaccttgc cggtctggag atggaaggcg cgaaggccgt gtccgcccgg
     3661 gacccagacc ccgctcggca gcttctgggc catggcctcg gagccgtaga agacggcgcg
     3721 cgcgatctcc atctggctca gcatctcgcc gagcttctcc tggatgtgga ggaagccggt
     3781 gatgccgatg gcgtcggcca ccttcatgcc gagcccgcag aagaactcga gctggctcag
     3841 catgcgtgcg gatgtctgca cgttgagcag agcgccgtag tcggcgccca tggtgttgat
     3901 gacctcggcg ctgcccgggc cgccgtccac catcacccgg tcccacggga ccagcacgtc
     3961 ctcgaacacc gcgatgcagt ccatctcctc gaagcggctc gagagcggat ggtcgaagcg
     4021 cgagcggggc agcggcgaca ccgtctcgcg gcagatgaac gtgaggccgg gggtggagga
     4081 tgggatgccg aaggcgagcg cgtaggcgtc gtccccgggg gccacgccgc cgaacgggat
     4141 caccgcgacc tcctcggtga tcggggccat ggtgcccagc atcttggccc cgcgcaccac
     4201 gatgccgtcg ggcgtctcgc gcacccggcc caggtggagg taggggtcct cctgctgggc
     4261 cgaggtcttg ctacgatcga tctgcgggtt gatcagcatg tgggtcagga agagatcgcg
     4321 ctcccgcacg tactcgtagt agcgcgtggc gttctcgccg aagcgggccc ccgcgcgggc
     4381 gaagcggtcc gccatcaggt gccagccggt gacgaactgg ttcatgaagt cgggcgagcg
     4441 ccccatgaag ccgaagttgt ggtcggcgcg cagcttgaag tgcttgcgtt tcttcaccag
     4501 ctcttcgcgc gagtagggga cgaggaacga cgtggacacg ggatcgcccg tcgtcggcga
     4561 cggataggtc atcacgtccc cgtacgccgg atcgagctgc atgtcgtact gctcggcgat
     4621 ggcgcggatc ggctcctgga agaccggctc ggcggtgacg tcggtgatgc gccggccgcc
     4681 caggaagacg cggggcggct gcttcttgag cgactcgatg tattcggctc cgctccgggc
     4741 tggcatgggg ctcacccttt cacgaaaggc gcggtgaagt caaccgacgg gtcgacagcg
     4801 cccgcggacg atggcagaat cggcggccgg gaggagagag ccatggacgc caacgtgaag
     4861 aagaccgcgc tacgcatgat cccctacgga ctctacgtgc tgaccgcgaa gggcaaggac
     4921 gacacggtcg cggcggccac cgtgaactgg gtgacgcagg cgtcgttcac cccgccgctg
     4981 gtggtggtgg gcgtgaagac cgactcgcac gcccacgcgc tcatcaagga gtcgaaggcc
     5041 ttcgcgctca acgtgctggg caagggccag caggccatgg ccttcacctt cttcaagccc
     5101 gccgcgcgcg aggggcagac cgtgtccggc gcgccgttcc gctggggcag taccggcgcg
     5161 cccatcctcg agcacgcgcc tgcgttcgtc gagtgccggc tcgtggacac ggtcgagcgt
     5221 ggggaccact cggtgttcgt gggcgaggtg gtggaggccg gcctggggaa gccggtcgag
     5281 gggcgggccg atgacgctac gctctggctg aaggagctgg ggccgaacgt cttctacggc
     5341 ggctagcgtc gccccctcac cctgccctct ccccctcggc gggggagagg ggctctccat
     5401 ttcccgctcc cctccgggga gagggcaggg tgaggggccc tacgccggcc gcggcgcgag
     5461 cttcagcagg tctccgatgg tggcggaggc cttgagcccg gagccggtga gcaccagcac
     5521 cgtgtcctcc tgcgggcgga tgacgcccgc agtgagcagg cggctcaggc cggccgcggc
     5581 caccgccgag gtgggctcga cgtagaggcc gcgccgggcg aggtcgcgca gggcgtggac
     5641 gatctcggcc tcctcgaccg cgacgatgct gcccccggtg tcgcgcaccg cgcgcagcac
     5701 gtcggccacg cgggtgggcc tgggcgtggc gatcccctcc gccacggtgg gcgcgatctc
     5761 ggcggccacg aggtgctcga cgcccgcccg gaacgcggcc gcgtagggcg cgcaccgcgc
     5821 ggcctgcacg ccgaagagcc gcggccggcg cgcgatctcg cccgcgcgga gcagctcgtc
     5881 gaagccgcgc tcggccccca gcacgttgct gccgtagccg agcggcacca ccacgttgtc
     5941 gggggcgcgg aagcccgcct gctcccacag ctcgtaggcg agggtcttcg tcccttccac
     6001 gaagaagggc tgccagttgt ggctcgcgta gaagatgtcc gcgctctggc gcagggccgc
     6061 ctccgccacg tcctggcgcg agcctcggat cgtcaccacg tcggcgccac aggccgcgat
     6121 ctgggcgatc ttgggatagg acgcggtctc cggcaccagg atgcggcagg tgaggcccgc
     6181 cgccgccgcg taggccgaca gcgacgcgcc cgcgttgccc gaggagtcct ccagcacgtg
     6241 ggtcacgccg cggctcttga gatagctcac catcacggtc atgccgcggt ccttgaagga
     6301 gccggtcggc atcatgaact cgagcttgta gtggacgggc gcgccgtgcc actccccggg
     6361 gaggagcggc gtccatcctt cgcccaggct caccgcgtgc tcggcgccga ccagcagccc
     6421 gcgcgcgtag cgccataccg agcggcgcgc ggtgtcgatg tcgcgcggcc ggagcccggg
     6481 gccgggcgcg aggttcaggt agtggccgtt gtcggcccgc cagcgcgcct cgctcagcgg
     6541 ataggtctgg ccggtcgagg gatcgacgta gtgcacggcg gcgccccgtc agtgcgttgc
     6601 ttcgagcgcg gtgagggcca gcacgcgcgt gcacaggaag agatccgcca ggtccacgtg
     6661 cacgtcgtga tagtcggtgt agccgccgcc cggtccgtag aggcagcagg ggatgccggc
     6721 ggcccagagg tgcgtggtgt cgttgcccgc gtagctctgg ggtagccgga cgccgaggtg
     6781 ctcgggctcg gctccggtca tcaggtggag gttgcgccgc accatctgta cgatgggcga
     6841 ggtcttcggc acgtccaccg cggccttggc gagccggccg agcccgcgct cgggccgggg
     6901 cgggaactcg atctcgaact cgaactttgg atcggcggcg gcgagggcgt cgagggtttg
     6961 ccggatgtcg gccaccaccg tctgcccggt ctggctcgcg ttgaagcgca cgttcacgta
     7021 ggcggtgcag aagtcgctca cggtgtaggc gccgcgcagg ttgtggtcgc ggccccgccc
     7081 gccgatgatc gagcccacgt tgagccgcgg cagcccgggc aggtccgggt cgtgggtgtg
     7141 ggtgaagcgc atggcgtgaa gcgcttccac cgcccgcatc atcatcgcga tcgcgtccac
     7201 cccgtgctcc ttcttgctga tgtgggtggc gcggcccagg gtgtggatca ccacgtccat
     7261 cgtcccggtg tgcttggtca ggatcgtccg tgccccgtag ggctcggcca ccaccgccat
     7321 gtccgtcatc tgtccattct cgagcgcgtg gatcgtgccg atgccgctgt tcagctcggc
     7381 ggccacgcag gccagcacca cgtcgccgcg gatcggcacc cgcgcgcgct ggagcgccag
     7441 cgtggccatg atgcaggcgg tgaccccggc cttcatgttg tagacgccgt ggccgaagag
     7501 cttgccgtcg gcgatccacg ggtcgaaggg gtcgcgcgtc cagccggagg cgagcggatc
     7561 gatgtcgatg tggccgttga gcatgatgga ccggccgccg ccggttccgc gcagccgcgc
     7621 gatggtctgc ttgcggccgg gctcgacttc ctggagcgtc accgggaggc cctggtcggc
     7681 gaggtagctc gcgagccact ccgcgagcgg ggtctcctcg gtggtgaacg aggggatccg
     7741 gatcagctcg caggcgaggc ggaccacctc gtcgtcgtcg atctgctcga ggaccctggc
     7801 tctgagctcg gcgggcaccg cgtcggccat cgtcgtcaca tattggccga tcaccggcgg
     7861 ctgtcaaggc ggttgacagg ctctcccgcg ccgcggtaag accggagcgc gccgggaggg
     7921 cgcgagccat gtccgcatcc gattcgatca cgatagacgc gccgcgcttc tggtccaccg
     7981 tcatgcgctc cggcgagatc ggccccggca agtcgggcgg cctccgccgg ctggccctca
     8041 ccgacgcgga caaggagatg cgcgatctct tcatcacctg gtgcacggag gccggctgta
     8101 cggtcgcggt ggaccgggtc ggcaacatct tcgcccggcg ccccggagtg gacgaccgcc
     8161 tgccgccggt gctgatgggc agccatctcg acacccagtt cgcgggcggc aagtacgacg
     8221 gcatcgtcgg ggtgctggcc ggcctcgagg tgcttcgcac gctggacgat cgccgcgtgc
     8281 gtaccaagcg cccgctcgag ctggtgtgct ggaccaacga ggaaggcgcg cgcttcacgc
     8341 cgccgatggt ggcctcgggc gccttcgcgg gggtcttcga cgtggactgg gtgctcgccc
     8401 tgcgcgacga cgacggcaag cagttcggcg gcgagctcga gcgcatcggc tacaacggca
     8461 aggccgcggt gggcggccgc tcgatcgacg cctacttcga gctgcacatc gagcaggggc
     8521 cgatcctcga tcgcgaggcg atcccggtgg gcatcgtggt gggcggttac gcggcgcacg
     8581 ggatgcacgt ggacgtgcac ggcgagaccg cgcattcggg gccgaccccg atggaccgcc
     8641 ggcgcaacgc cctggtgggc gcggcgatgc tcgcggtcgc ggtcaacgag atcggctgga
     8701 agtaccacgc caccgaaggc aaggccacgg tggcccgcct ggtggcgtgg cccaacaagg
     8761 cgggcatcct cgccgagtac gcgcaggtca cctgcgacgt gcggcacgcc gatcgcgcgg
     8821 tggccgatca gatgtgggcg gaggtgaagg cggcgatggc ggagtgcgcg gggcgagcca
     8881 acgtggagat ggccatcgcg ggggagtggc acttcggcag cgaacgcttc gatcccggct
     8941 gcatccggct gatccgggac gcggcccggg agcggggcgt cgcccaccgg gacatcctga
     9001 gccaggccgg gcacgacgcg tactacatct cccggattgc accgaccgcg atgatcttca
     9061 ccccgtgccg cgacggcatc tcgcacaatg aggccgagca cgccgagctc gactacacct
     9121 cgccgggcgt gaacgtgctg ctggacgcgg tgctggcccg ggccaaccgg tagaggcgcg
     9181 cgcgattccg gatccgcagc gcctgctgct ccacccgccg ttcgcgctgc tgtcgtcgtc
     9241 ccgggtcctg gccaccgtcg gcttccagat gctaggggtc gcggtgggct ggcagctcta
     9301 cgagctgacc gggagcgcgc tcgacctggg gctggtcggg ctcgtgcagt tcctgccgat
     9361 gatcctgctc accctggtcg tcgggcaggt ggccgaccgc tgcgaccggc gcctcatcgt
     9421 gatcgcgtgc gagatcgtga aggcggtcgc cgccgccgcg ctcgccctgg gggccctcgg
     9481 ggg
//
LOCUS       DASUFA010000361         7025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_769822, whole genome shotgun sequence.
ACCESSION   DASUFA010000361 DASUFA010000000
VERSION     DASUFA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769822"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFX87_13210"
     CDS             <1..756
                     /locus_tag="VFX87_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX4995933.1"
                     /translation="LKEWKWPEHAVPTYARSHVHWREMNRAAGEMALLPTFRLPTLIH
                     SRSGNAKWLYEISHNNPLWLHPEDAARWDVKTGDLLKVTTEIGHFVDRVWVTEAIRPG
                     VVACSHHLGRWRLAEQTGGERWSTALVTLENPRAGEWRLRHVHGVRPFESADPDSQRV
                     WWEDAGVHQNLTFPVHPDPVSGQHCWHQKVTVSRPGPNDRYGDVVVDTTKSFEVYRKW
                     LAMARPAPGPGGLRRPLWLPRAFKPDASAYRIE"
     gene            797..1282
                     /locus_tag="VFX87_13215"
     CDS             797..1282
                     /locus_tag="VFX87_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX4995934.1"
                     /translation="MTATLTRRAWLGLAALGAAVAAWRPWPARAQGHLANLAPEEPIA
                     ETMKRLFGNRTIKDGASLITIDMPLIAEDGASVPIAVEVKSPMTAESHVKAIYILAEK
                     NLRPLNVKISLTPAAGQAYVGTNLRLADSGPVRVVAETGDGALWIATRDVRVAVAGCA
                     A"
     gene            1286..1615
                     /locus_tag="VFX87_13220"
     CDS             1286..1615
                     /locus_tag="VFX87_13220"
                     /inference="COORDINATES: protein motif:HMM:NF020350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX4995935.1"
                     /translation="MADIGTVKLAVPGSVRRGSVARVRTLVIHPQERVERQGGRSVDR
                     NYRFVNRVVVTYLGREIAVIEPSTAVSENPTFTIPFRATEAGQLKVTFFDSHGVKFEG
                     TAEVKVT"
     gene            1612..2160
                     /locus_tag="VFX87_13225"
     CDS             1612..2160
                     /locus_tag="VFX87_13225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4995936.1"
                     /translation="MRLGLLAALLAGSLAWAAPASADEIDRYLRELQLNALPGERAPA
                     FSLPKVDGGGKTSLAEQQGKVVLVYFWATWCGYCRKELPFAIEKIVRERRGQPFTVLA
                     VSIEEPRDLVASYVKGAGVTSSVLLDGDGAVAREYRVRATPTTIVIGRDGRLVARAAG
                     TREWDGKAGRAFLDALVAAPAK"
     gene            2180..3925
                     /locus_tag="VFX87_13230"
     CDS             2180..3925
                     /locus_tag="VFX87_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4995937.1"
                     /translation="MAADLVLDSLDTDILVLGAGGAGLCAALHAADRAPGLTVTVAVK
                     GLLGRAGCTRMVQGGYNAVLEAPDSLEAHLLDTLQGGAWINDQELVWTLVHEAPGRVL
                     EMEARYGCFFDRHPDGRIHQKPFAGQSHDRTIHKGDLTGIEIMNRLTEQVWRRPSIRP
                     LEEHRAVELLRADGGQVGGVLLLDVRRGTFVVVRARAILLAMGGGPTMYKVIACSADK
                     SSDGIALGLHAGVRLRDMEMVQFHPTGLIVPNSLMTGALLEEGLRGAGGRLLNGRGER
                     FMQRYDATRLERSTRDLVARACHTEVLEGRGTANGGVWIDVSHLGAEVVEKSFRGMVR
                     RCRDFGRDLAREPVEVGPTAHFMMGGIVIDRACRTDVEGLFAAGEDTGGVHGANRLGG
                     NGVAESTVFGGIAGDVMADWVVGRPLPQLSRAHVDAAVAATAAPLGRSEGGQSLYALQ
                     ARLRQVMWDHVGLIRAGEGLKLALTEIEDISSRLESVAVRGGGAYNLAWQDWINLQNQ
                     ATTAWLIARSALERTESRGSHYRRDFPTTAGEPVAVFAGFADGTGPKVWTEPVRYTRL
                     KPDAARRPTTVDVGD"
     gene            3925..4254
                     /locus_tag="VFX87_13235"
     CDS             3925..4254
                     /locus_tag="VFX87_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX4995938.1"
                     /translation="MPKRPRRILRRRWATTEGWRGTRAGMWAWLVQRTAAVALLAVIV
                     LHVRTPYWRTVQELLLALVLVHGLLGARAILLDFGLPVRWHRLLLVIAVALAVLIFAT
                     VWGWRWS"
     gene            4245..4940
                     /locus_tag="VFX87_13240"
     CDS             4245..4940
                     /locus_tag="VFX87_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX4995939.1"
                     /translation="MVLKVFRWSPGFRERIQRFWVPAKEGMTVLDALVDAQRRLDPTL
                     AFRYACRVGMCGSCGVVINGRERWACRTLLKSLETRIVTVRPLYHFPLIRDLVVDLAP
                     LAEQTARVGATFVPKEEPTAFARVGAGAPERQRIDPAIECIGCGMCVSACTMVANDER
                     FPGPAALNRLFTVDTDSRDAGHAERAAVLFSEDTLMRCHSQANCTDVCPMEISPTESI
                     LALRRRAVRGLLG"
     gene            4951..5388
                     /locus_tag="VFX87_13245"
     CDS             4951..5388
                     /locus_tag="VFX87_13245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX4995940.1"
                     /translation="MSPDPAAGCLFCRIARSEEPGALVLETRAVAAFLDHRPLFPGHC
                     LVVPRAHVETIGDLPATLTAPLWDAVRVLARAVEGALGADGSFVAVNNRVSQSVPHLH
                     VHVVPRRRGDGLRGFFWPRGRYAGPAEMEDVRRRIAAAVAAAD"
     gene            5533..>7025
                     /locus_tag="VFX87_13250"
     CDS             5533..>7025
                     /locus_tag="VFX87_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX4995941.1"
                     /translation="MSVDQVKRLYESMPGKLDEARRRFGRPLTLAEKILVAQADDFAR
                     QEWSRGRAQLRLRVDRVALQDATGQMALLQFMQAGKKQVAVPSTVHCDHLIRAESGAA
                     ADMERAIAENREVYDFLHSTSKKYGIGFWKPGAGIIHQVVLENYAFPGGLMIGADSHT
                     PNAGGLGMAAIGVGGADCGEAMAGLAWEVLDPRLIGIHLTGKLAGWSSPKDVITYLCG
                     LLTVKGGTNKIVEYFGPGAESISATGKGTICNMGAELGATTSLFHFDDRMAAYLRATD
                     RADIARLAEQHRQHLVSDPEVLRDPRKFYDEVVEINLSELEPHIVGPHSPDRARPISK
                     LAAEVKKEGWPAQIRQTLIGSCTNSSYEDMRRAAHIAMQALRAGVKAKTPFLVTPGSE
                     RIYQTIKRDGIMDTFQKIGGTVLANACGPCIGQWKRSDVGKEETNTIVSSFNRNFPGR
                     NDGSASTLSFLTSPEIVTAMAFAGTLEFDPVNGTIPGPDGKAFRFTAP"
BASE COUNT          985 a         2499 c         2566 g          975 t
ORIGIN      
        1 ctcaaggagt ggaagtggcc ggagcacgcg gtgcccacct acgcgcgcag ccacgtgcac
       61 tggcgggaga tgaaccgggc ggccggcgag atggcgctcc tgcccacgtt ccggctgccg
      121 accctgatcc actcgcgctc cggcaacgcc aagtggctct acgagatctc ccacaacaac
      181 ccgctctggc tgcatcccga ggacgcggcg cgctgggacg tcaagaccgg cgatctcttg
      241 aaggtcacga ccgagatcgg ccacttcgtg gaccgcgtgt gggtgacgga ggcgatccgg
      301 ccgggcgtgg tggcgtgctc gcatcatctg ggccgctggc gcctcgccga gcagaccggc
      361 ggcgagcgct ggtccaccgc gctggtgacg ctcgagaacc cgcgggcggg ggagtggcgc
      421 ctgcgtcacg tgcacggcgt gcggcccttc gagagcgccg atcccgattc gcagcgcgtc
      481 tggtgggagg acgcgggcgt gcaccagaac ctcaccttcc cggtgcatcc ggatccggtg
      541 agcggccagc actgctggca ccagaaggtg acggtgagcc ggcccgggcc gaacgaccgg
      601 tacggcgacg tggtggtgga caccaccaag tccttcgagg tctatcgcaa gtggctcgcc
      661 atggcccggc ccgcccccgg ccccggtggc ctgcgccggc cgctctggct gccgcgcgcg
      721 ttcaagcccg acgcatcggc ctaccgcatc gagtgaagcc gttgacaagg acgcgatcga
      781 aggcatcctg ggagccatga ccgccacgct gacgcgtcgc gcatggctgg gcctggccgc
      841 gctcggcgcc gccgttgcgg cgtggcgtcc gtggcccgcc cgcgcgcagg gccacctcgc
      901 caacctggcg ccggaagagc cgatcgccga gacgatgaag cggctcttcg gcaaccggac
      961 gatcaaggac ggcgcctcgc tcatcacgat cgacatgccg ctcatcgcgg aggacggcgc
     1021 ctcggtgccg atcgcggtgg aggtgaagtc gccgatgacg gcggagagcc acgtcaaggc
     1081 gatctacatc ctcgccgaga agaacctgcg gccgctcaac gtgaagatca gcctgacgcc
     1141 ggccgccggc caggcctacg tggggaccaa cctgcgcctg gccgacagcg ggccggtccg
     1201 cgtggtcgcc gaaaccggcg acggggctct gtggatcgcc acccgtgacg tccgcgtcgc
     1261 ggtcgccggc tgcgcggcct gagccatggc cgatatcggg accgtgaagc tggcggtgcc
     1321 gggcagcgtc cgccggggca gcgtggcgcg ggttcggacc ctcgtgatcc atccccagga
     1381 gcgggtggag cggcagggcg ggcggtcggt ggatcgcaac taccgcttcg tcaaccgcgt
     1441 cgtggtaacg tacctcggcc gcgagatcgc cgtgatcgag ccgtccacgg cggtcagcga
     1501 gaacccgacg ttcacgattc cattccgggc cacggaggcg gggcagctca aggtgacgtt
     1561 cttcgactcg cacggcgtga agttcgaggg caccgcggag gtcaaggtca cgtgaggctc
     1621 gggctcttgg cggcgctgct ggcgggaagc ctggcgtggg ccgcgcccgc gtcggccgac
     1681 gagatcgacc ggtacctgcg cgagctccag ctcaacgccc tgcccggcga gcgggcgccc
     1741 gcgttctcgc tgccgaaggt ggacggcggc gggaagacgt cgctggccga gcagcagggc
     1801 aaggtcgtgc tggtctactt ctgggccacg tggtgcggct actgccggaa ggagctgccc
     1861 ttcgccatcg agaagatcgt ccgcgagcgc aggggacagc cgttcaccgt gctcgcggtg
     1921 agcatcgagg agccccggga cctggtggcc agctacgtga agggcgcggg cgtgacctcg
     1981 tccgtgctcc tcgacggcga cggcgcggtc gcgcgcgagt accgggtgcg ggccaccccg
     2041 accaccatcg tgatcggccg cgacgggcgc ctggtggccc gggccgcggg cacccgcgag
     2101 tgggacggca aggccggccg cgccttcctc gacgccctgg tggccgcgcc ggcgaagtaa
     2161 cctcggggcc acccgaccga tggccgccga tctcgtcctg gacagcctcg ataccgacat
     2221 cctcgtgctc ggcgcgggcg gcgcggggct ctgtgccgcg ctccacgccg cggatcgcgc
     2281 tccgggcctc acggtgaccg tggcggtgaa ggggctgctc ggccgcgccg gctgcacgcg
     2341 catggtgcag ggcggctaca acgcggtcct cgaggctccc gactcgctcg aggcgcatct
     2401 cctcgacacg ctgcagggcg gggcgtggat caacgatcag gagctggtgt ggaccctggt
     2461 gcacgaggcg cccggccgcg tgctcgagat ggaggcccgc tacggctgtt tcttcgaccg
     2521 ccatccggac gggcgcatcc accagaagcc cttcgcgggg cagagccacg accgcaccat
     2581 ccacaagggc gatctcaccg gcatcgagat catgaaccgc ctgaccgagc aggtctggcg
     2641 gcgcccgtcg atccgcccgc tcgaggagca ccgggcggtc gagctgctcc gcgcggacgg
     2701 cggccaggtg ggcggcgtgc tgctgctcga cgtccgccgc ggcaccttcg tggtggtgcg
     2761 cgcccgcgcc atcctgctcg cgatgggcgg cggccccacc atgtacaagg tcatcgcctg
     2821 ctcggccgac aagtcctccg acggcatcgc cctcggcctg cacgcgggcg tgcgcctgcg
     2881 cgacatggag atggtgcagt tccacccgac cgggctcatc gtgcccaact cgctcatgac
     2941 cggcgcgctg ctcgaggagg ggctgcgcgg ggcgggcggc cggctcctca acggccgcgg
     3001 cgagcggttc atgcagcgct acgacgccac gcgcctcgag cggtccaccc gcgacctggt
     3061 ggcgcgcgcc tgccacaccg aggtgctgga aggccgcggc accgccaacg gcggcgtgtg
     3121 gatcgacgtc tcgcacctgg gcgcggaggt ggtggagaag agcttccgcg gcatggtgcg
     3181 gcggtgccgc gacttcgggc gcgatctcgc gcgcgagccg gtcgaggtgg ggccgaccgc
     3241 ccacttcatg atgggcggca tcgtgatcga ccgggcctgc cgcaccgacg tggagggcct
     3301 cttcgcggcc ggcgaggaca ccgggggcgt gcacggcgcg aaccgtctcg gcggcaacgg
     3361 ggtcgcggag tccaccgtgt tcggcggcat cgcgggcgac gtgatggcgg actgggtggt
     3421 ggggcgcccg ctgccccagc tctcgcgcgc gcacgtcgac gcggcggtcg cggccaccgc
     3481 cgcgccgctc gggcgcagcg agggcggcca gagcctctac gccctgcagg cgcggctccg
     3541 gcaggtcatg tgggatcacg tggggctgat ccgcgcgggc gaaggcctca agctggcgct
     3601 gaccgagatc gaggacatct cgagccggct cgagagcgtc gcggtgcggg gcggcggggc
     3661 ctacaacctc gcgtggcagg actggatcaa cctccagaac caggccacca cggcgtggct
     3721 catcgcccgc agcgcgctcg agcgcaccga gagccgcggc tcgcactatc gccgcgactt
     3781 cccgacgacg gcgggcgagc cggtggcggt gttcgccggc ttcgccgacg gcacgggccc
     3841 caaggtctgg accgagccgg tgcgttacac gcgcctcaag ccggacgcgg cgcgccggcc
     3901 cacgacggtg gacgtcggcg actgatgccg aagcggcccc ggcggatcct gcggcgccgg
     3961 tgggccacca ccgagggatg gcgcggcacc cgggccggga tgtgggcctg gctggtgcag
     4021 cgcaccgccg cggtggccct cctcgcggtg atcgtgctcc acgtgcgcac cccgtactgg
     4081 cgcacggtgc aggagctcct gctggctctc gtgctcgtgc acggcctgct cggcgcgcgg
     4141 gccatcctgc tcgacttcgg gctgccggtg cgctggcacc ggctgctgct cgtgatcgcg
     4201 gtggcgctgg ccgtgctcat cttcgccacg gtctggggct ggcgatggtc ctgaaggtct
     4261 tccgctggtc cccgggcttc cgcgagcgga tccagcgctt ctgggtgccc gccaaggagg
     4321 gcatgacggt gctcgacgcg ctggtggacg cccagcgccg tctcgatccg accctggcct
     4381 tccgctacgc ctgccgcgtg ggcatgtgcg gctcgtgcgg cgtggtcatc aacgggcgcg
     4441 agcgctgggc ctgccgcacc ctgctgaaga gtctcgagac ccgcatcgtg accgtgcggc
     4501 cgctctacca ttttccgctg atccgcgacc tggtggtgga cctggccccg ctcgccgagc
     4561 agaccgcgcg ggtgggcgcc accttcgtgc cgaaggagga gccgacggcc ttcgcccgcg
     4621 tcggcgcggg cgccccggag cggcagcgca tcgaccccgc gatcgagtgc atcggctgcg
     4681 gcatgtgcgt gtcggcgtgc acgatggtcg cgaacgacga gcgcttcccg ggcccggccg
     4741 cgctcaaccg cctcttcacg gtggacacgg acagccgcga cgccggccac gccgagcgcg
     4801 cggcggtgct gttctcggaa gacacgctca tgcgctgcca cagccaggcc aactgcaccg
     4861 acgtctgccc catggagatc tcgccgaccg agtccatcct ggcgctgcgg cggcgcgcgg
     4921 tgcgcggcct cctcggctga ccgcgatccc gtgagcccgg acccggccgc gggctgcctc
     4981 ttctgccgca tcgcgcgctc agaggagccg ggcgccctcg tgctcgagac tcgcgcggtg
     5041 gccgcgttcc tcgatcaccg gccgctcttc cccggccact gcctcgtggt gccccgcgcg
     5101 cacgtggaga ccatcggcga tctgcccgcc acgctgaccg cgccgctctg ggacgcggtg
     5161 cgcgtgctcg cgcgggcggt ggagggggcc ctcggcgccg acggctcctt cgtggcggtc
     5221 aacaaccggg tgagccagag cgtgccgcat ctgcacgtgc acgtggtgcc gcggcggcgg
     5281 ggcgacgggc tgcggggctt cttctggccg cgcgggcggt acgcgggccc cgcggagatg
     5341 gaagacgtgc gccggcggat cgcggcggcg gtggccgcgg ccgactgaag cgcgggttac
     5401 cgccgcccga ggctcgttcc cttgacctct ccgggtcggc atgctagcgt aactgcggat
     5461 tttatctggt cgcctcctgc ctgaccgcgg gtgggaggcg atacgtatgt ctgccggtcg
     5521 aggaggccca ccatgtcggt tgaccaggtc aagcggctgt acgaatccat gcccggcaag
     5581 ctcgatgagg cgcgccggcg cttcggccgc cccctcaccc tggccgagaa gatcctggtc
     5641 gcgcaagccg atgacttcgc caggcaggaa tggagccggg gccgagccca gctccggctg
     5701 cgggtggacc gggtggccct ccaggacgcc accggccaga tggccctcct gcagttcatg
     5761 caggcgggca agaagcaggt ggcggtgccc agcacggtgc actgcgacca cctgatccgg
     5821 gcggagagcg gcgcggcggc ggacatggag cgggccatcg cggagaaccg cgaggtctat
     5881 gacttcctgc actcgacctc caagaagtac ggcatcgggt tctggaagcc gggcgccggc
     5941 atcatccacc aggtcgtgct ggagaactac gccttcccgg gcgggctcat gatcggggcc
     6001 gactcccaca cccccaacgc gggcggcctc ggcatggccg ccatcggggt gggcggggcc
     6061 gactgcggcg aggccatggc cgggctggcg tgggaagtgc tcgatcccag gctgatcggg
     6121 atccacctga ccggcaagct cgccggctgg agctcgccca aggacgtgat cacgtatctc
     6181 tgcggcctgc tgaccgtcaa gggcgggacc aacaagatcg tcgagtactt cgggccgggc
     6241 gcggagtcca tcagcgccac cggcaagggc accatctgca acatgggcgc ggagctgggg
     6301 gcgaccacct cgctcttcca cttcgacgac cggatggcgg cctacctccg cgccaccgac
     6361 cgggccgaca tcgcgcggct ggccgagcag caccgccagc acctggtgtc cgatccggag
     6421 gtgctgcgcg acccgcggaa gttctacgac gaggtcgtcg agatcaacct ctccgagctc
     6481 gagccccaca tcgtgggccc ccactcgccg gaccgggcgc ggccgatctc caagctggcc
     6541 gcggaggtga agaaggaagg ctggccggcc cagatccggc agaccctcat cggctcctgc
     6601 accaactcct cctacgagga catgcggcgg gccgcccaca tcgcgatgca ggcgctccga
     6661 gccggggtca aggccaagac cccgttcctg gtgacgccgg gctcggagcg catctaccag
     6721 accatcaagc gcgacggcat catggacacc ttccagaaga tcggggggac cgtgctcgcg
     6781 aacgcgtgcg ggccgtgcat cgggcagtgg aagcgcagcg acgtgggcaa ggaggagacc
     6841 aacacgatcg tctcctcgtt caaccgcaac ttccccggcc gcaacgacgg cagcgcctcc
     6901 acgctctcgt tcctgaccag cccggagatc gtgaccgcga tggccttcgc cggcaccctc
     6961 gagttcgatc cggtgaacgg caccatcccc ggcccggacg gcaaggcatt ccgcttcacc
     7021 gcccc
//
LOCUS       DASUFA010000362        11815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_899087, whole genome shotgun sequence.
ACCESSION   DASUFA010000362 DASUFA010000000
VERSION     DASUFA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899087"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFX87_13255"
     CDS             <1..834
                     /locus_tag="VFX87_13255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX4995942.1"
                     /translation="SYNSTYPYAASGRFTGDWTNGYLEPIAMMGVLVGLTSHVKIGTS
                     VLVVPYRNPVVTAKMLATLDMMSGGRIILGAGVGWMKEEFETLQAPPFTERGKVTDEY
                     LRLMRDMWTKEPVEFMGAHYRLPSVSALPKPRQKGGIPIWIGGHTDVALRRTGELADA
                     WHPIAMRPPAMLLPDEYAEKVKVIHGWARKVGRDPKAITLSVRVPLELAPRRGKAAVS
                     DQPGFRGTAQDIIRHVKSYQALGVSHFVFDLAPQDLRGQLSLMERFAQEVRPKVLRTP
                     R"
     gene            849..1292
                     /locus_tag="VFX87_13260"
     CDS             849..1292
                     /locus_tag="VFX87_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX4995943.1"
                     /translation="MSPTAPIPPPVAGFITSARVGRLATADAGGQPMVVPICYAFDGQ
                     VLYSAVDAKPKQTPPEGLKRIRNLRDNPRVSIVIDQYDEDWTRLRYVIIQGRADLLTD
                     GPQFAHGVDLLVAKYAQYRTLGLPRERGLMIRVTPSRVSSWQYSS"
     gene            1274..2476
                     /gene="cofG"
                     /locus_tag="VFX87_13265"
     CDS             1274..2476
                     /gene="cofG"
                     /locus_tag="VFX87_13265"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX4995944.1"
                     /translation="MAVLELNPVERALDRVAEGRTPDAAEATALLDTPVVRLPALLGA
                     ASALRDRGRGRRITFSAKVFIPLTTLCRDYCGYCTFRKDPGEPGAFTMTPDQVLALAR
                     AGARLGAKEALFSLGDKPEAIFPEHRAFLRSVGHRTTLGYLREISARVLDETGLLPHA
                     NPGLMTERDLASLREVNVSMGIMLESTSERLLAPGAAHDRAPDKVPQRRLRTIARAGA
                     LGIPFTTGILIGIGETHAERVDSLLAIRDLHARHGHIQEVIIQNFRAKATIPMRGWPE
                     PGALDLLRTVAVARLLLGPGMNIQAPPNLSADGYDQLPAAGLNDWGGISPLTPDHINP
                     ERPWPGLAELRRRTEAAGHELRERLAVYPEYTTREAFLDERLRSRVAGLVDAEGFVRP
                     DLEIWRHW"
     gene            2476..3756
                     /gene="cofH"
                     /locus_tag="VFX87_13270"
     CDS             2476..3756
                     /gene="cofH"
                     /locus_tag="VFX87_13270"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX4995945.1"
                     /translation="MPTDKPTTISLDWASPDTRRILGALLDGAELSVADGIRLTETRG
                     RDLQALTLVADEMRRRQAGDVVTYVVNRNINFTNVCIKHCTFCAFSRDHREEEGYFLP
                     MEEVVRRAIEAWEMGATEVCIQAGLPPKLDGRYYVDLCRAIKAALPEMHLHAFSPEEI
                     LYGSTRSGLSIPEYLAELKKVGLGTLPGTSAEVLDQEIRDVIARGRITVKQWIEVITA
                     AHAQGIRTTSTIMYGHVERPEHWVRHMDLLRGIQKETGGFTEFVPLSLIHQEAPMYRR
                     GLVPGVRQGATGLEVIRMHALARLFLGPLFRNIQCSWVKEGPKLAQVLLAAGCNDMGG
                     TLINESISTSAGASYGQLVPPVELRRLIRDAGRVPAQRSTTYELLRVYEVEDPADDSP
                     LDHVADAEARFGSYRALAGSGQFRFLHPTRAPGD"
     gene            3914..4189
                     /locus_tag="VFX87_13275"
     CDS             3914..4189
                     /locus_tag="VFX87_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX4995946.1"
                     /translation="MTKSAIIAHLAQKTSLTKKQIVDVMDQLLSLATKEAKNVFVVPN
                     FGRLVLANRKARMGRNPQTGEPIKIPAKRVVKFRLAKSLKDSVLGKK"
     gene            4329..4682
                     /locus_tag="VFX87_13280"
     CDS             4329..4682
                     /locus_tag="VFX87_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995947.1"
                     /translation="MGAVLMERAGATAMLHGPVVVAPPDTPTEDVLAVATELVNQALA
                     HAGAAGIATVFARPQSLDRVWVRSGFIPIPEAELPRELQGRPGLGLFGWRGGTALWST
                     AGRGGSRSALARGEH"
     gene            4701..5627
                     /gene="cofD"
                     /locus_tag="VFX87_13285"
     CDS             4701..5627
                     /gene="cofD"
                     /locus_tag="VFX87_13285"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX4995948.1"
                     /translation="MKLTALAGGTGAAKRLRGLSRIMDPRDLTVIVNTGDDAEIWGLH
                     ISPDLDTVSYTLGGVIDEQKGWGLTGETFHALDQIARFGEPVWFNLGDRDLATHLHRT
                     RLLREGRTLTDVTRSIAAALGVGATVLPMSDQPVRTRLLGPDGWLTFQEYFVREKAQV
                     EARAVDYVGAAAAAPAPGVVEAIAAADAVLVCPSNPITSVGPILAVPGLVEALGATSA
                     TVVAVSPIVGGAAVSGPAGRLMASAGLPVSAAGVARAYAAWLDRLVYDEQDRALAGEI
                     RALGVAPVPAPTMMSSREAEIALARHVLSAAA"
     gene            5624..6283
                     /gene="cofC"
                     /locus_tag="VFX87_13290"
     CDS             5624..6283
                     /gene="cofC"
                     /locus_tag="VFX87_13290"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX4995949.1"
                     /translation="MSLLLAVPVKDLVNAKQRLIPALSPSERRELARAMLEDVLDAAV
                     AALPGAVLVVTTDPEVQAVARAAGALCLTESVNRGHTAAVAFAQREAVARGAARFLTL
                     PGDVPCVTAQEIATLCGALAEAPGVVFVPSRSGQGTNAVLLAPPDAIPLTFGEPSFEN
                     HLKTARAAGLTPEVLELPGIGLDVDAPDDLPVLLERGPTTRSARLLRGFPAAARAGGA
                     R"
     gene            6286..7038
                     /gene="cofE"
                     /locus_tag="VFX87_13295"
     CDS             6286..7038
                     /gene="cofE"
                     /locus_tag="VFX87_13295"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX4995950.1"
                     /translation="MPPRYEVIGVQGLPEFRPGDDVAALIADAAGRQGTPIVARDLVV
                     VSQKIVSKAEGRLVRLSEVTPSPRARAVAEEIGRDPRLVEVILSESRRVVRQAKGVLI
                     VETHHGFVCANAGVDQSNVDADTACLLPEDSDRSAAALRERLQALTGHELAVIIADTF
                     GRPWREGLVNVAVGLAGFEPIRSYLGELDPAGYVLQATILALADELAGAAEPVMGKLD
                     RIPVAIIRGLDWERGDGSSRALLRDPARDLFR"
     gene            7058..7708
                     /gene="npdG"
                     /locus_tag="VFX87_13300"
     CDS             7058..7708
                     /gene="npdG"
                     /locus_tag="VFX87_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX4995951.1"
                     /translation="MRIGIVGGTGKEGVGLGLRWAQAGHEVIIGSRDPERARAKAAEL
                     AASTPGSRVDGMSNRDAAATADVIVLTVPATGLQTTLPELRDVCRGKIVVSTVVPLTF
                     GGGRLFTPPPQGSAAEEAQEILGPEARVVASFHHIAAHELAAGDHAIECDLLLCGGDA
                     PAKKLVTELGASMGLRAVDVGALTNAGPLEGITAVLATINRRYKLKNSGIKITGLP"
     gene            7723..8802
                     /locus_tag="VFX87_13305"
     CDS             7723..8802
                     /locus_tag="VFX87_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4995952.1"
                     /translation="MADTPITVSVAALTAYIVSVLEALKMSPRNAALSADLMVRTDLR
                     GVDSHGIGMLPKYVEWTRAGFIHPDAEPTVARDDMATALVDGQKGLGHPASVLAMELA
                     ITKAATYGIGIVAVRNSNHFGACANYSMMALERGMIGFAVTNSPFVAMVPTFARKPMM
                     GTNPLSLAAPAARHAPFVLDMATTTVAVGKLTIASRWNKPIPEGWGLDAHGRPTSDAR
                     EVLASRLLSPLGGTRELGGHKGYGLGVMVDVLSGVLSGGVYGNVLDRTNGRERKETNT
                     GHCFAAIDIKRFRPLGDFTAAMDDMLQALHDTPRADGQERIYTAGEPEAETERLRRTQ
                     GIPVAPALVRQCNDIATELGVKPLA"
     gene            complement(8970..9659)
                     /locus_tag="VFX87_13310"
     CDS             complement(8970..9659)
                     /locus_tag="VFX87_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4995953.1"
                     /translation="MKLVLFDIDGTLITSRGAGRRAMRTALERVFGAAGGIDQYDLRG
                     RTDTRIVHDVMGALGWEPARVKERLDDFFEAYVGGLTSEIGDGRDVVTLPGVSAVVAR
                     LAECPDVVLGLVTGNIEEGARIKLLPTGLWPHFRVGAYGSDHMDRRHLPSLATRRAHA
                     LVGYAFVPPEVVVIGDTPYDIDCARAFGAVAIAVTTGQYPRDELLADRPDHLFDDLGD
                     VEKVLAAVLGR"
     gene            complement(9677..10870)
                     /locus_tag="VFX87_13315"
     CDS             complement(9677..10870)
                     /locus_tag="VFX87_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX4995954.1"
                     /translation="MPVEPAGVEPGRVYLDYAGFAPVDPRVLAVTRPFLEAGIGNPSA
                     RHSLGAEARESLDAARVKVARLCGGAPGGIVFTSGATEANNLAIKGVVARARGGGPVR
                     VLLSAIEHISVINAFRDLEKAGVTVGYLPVDGEGRVDPGMLRAALTADTVLVSIAAAN
                     PEIGTVQPLKEIGRITRAAGVALHVDGVGALGRVPLNVEAMSIDLLTVSGNDLYAPPG
                     SGALWVRPGVRIHPQVLGGGQEGGYRSGTENVPGVVGLGVAAELMRVEGAHQEATRLA
                     TLRDRLMHGLLEAIPDCRLTGSATSRLPHHLSLVVRGVKADSVLLDLDLDGVAASSGS
                     ACASLTQTTSHVLRAIGCAPGEAEGSLCFTLGRWSTAADVEAVLDRLPPIVARLRALA
                     AALHT"
     gene            complement(10871..11122)
                     /locus_tag="VFX87_13320"
     CDS             complement(10871..11122)
                     /locus_tag="VFX87_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006209597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX4995955.1"
                     /translation="MALEAKVVNPDRQIDCTGLFCPMPIVKTREAMTQMARGQVLEML
                     SDDPASDPDMRSWAQNTGHELLEVTRNGAVYRFVIRKTR"
     gene            11230..>11815
                     /locus_tag="VFX87_13325"
     CDS             11230..>11815
                     /locus_tag="VFX87_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4995956.1"
                     /translation="MARRARAAQTPAARRIKRKILIAIALTAFLPMLVVAYLVYAYAL
                     PLLDPQAQERNLPWMLVMLGGTGLLMAAGSFMLWDLATSVVRHADSLDAPRRPAPAAG
                     ASRPAASPETTAVQQYVQQSDEVDSLMQSFSRMLVTIEQQASDVNEDARRLDTAYKEL
                     ESTSAQLKEFSFKDEVTGLYNRRFFSIRLEEEVSR"
BASE COUNT         1743 a         4364 c         4043 g         1665 t
ORIGIN      
        1 tcctacaact cgacctatcc ctatgcggcc agcggccgct tcaccggcga ctggacgaac
       61 ggctacctcg agcccatcgc gatgatgggg gtgctggtcg ggctgacctc ccacgtgaag
      121 atcggaacca gcgtgctggt cgtgccgtat cgcaacccgg tagtcaccgc caagatgctg
      181 gccacgctcg acatgatgtc gggcgggcgc attatcctcg gggccggggt gggctggatg
      241 aaggaggagt tcgagacgct ccaggcgccg cccttcacgg agcggggcaa ggtcaccgac
      301 gagtacctgc gcctcatgcg ggatatgtgg acgaaggagc ccgtggagtt catgggcgcg
      361 cactatcgcc tgccctcggt gagcgccctg cccaagccac gccagaaggg tggcatcccg
      421 atctggatcg gcggccatac cgacgtcgcg ctgcggcgca cgggcgagct cgcggacgcc
      481 tggcatccca tcgcgatgcg tccgcccgcc atgctcctcc ccgatgagta cgcggagaag
      541 gtgaaggtca tccacggctg ggcccgaaag gtcgggcgcg acccgaaagc catcaccctc
      601 tcggtacggg tgccgctcga gctggcgccc cggcgcggca aggcggcggt gagtgaccag
      661 cccggcttcc ggggcaccgc ccaggacatc atccggcacg tcaagagcta ccaggcgctc
      721 ggcgtctccc acttcgtctt cgacctggcc ccccaggatc tcagggggca gctctccctg
      781 atggagcgct tcgcccagga ggtccgaccg aaggtcctcc ggacgccccg ctagggcggc
      841 caacgccctt gagcccgacc gcgcccattc ccccgcccgt cgccggcttc atcacgagcg
      901 cgcgcgtggg ccggctcgcc accgccgacg cgggcggcca gccgatggtg gtgccgatct
      961 gctacgcctt cgacggccag gtgctgtact cggcggtgga cgccaagccg aagcagacgc
     1021 caccggaggg gctcaagcga atccgcaacc tcagggacaa tccgcgcgtc tcaatcgtga
     1081 tcgaccagta tgacgaggac tggacgcgtc tgcggtacgt catcatccag gggcgggcgg
     1141 atctgctcac cgacggaccg cagttcgccc acggggtgga cctgctcgtc gccaagtacg
     1201 ctcagtaccg caccctcggg ctgccgcgcg agcgaggcct catgatccgc gtgaccccca
     1261 gccgcgtctc ctcatggcag tactcgagct gaacccggtc gaacgggcgc tggatcgggt
     1321 cgccgagggc cggacgcccg acgcggcgga ggccaccgca ctcctcgaca ccccggtcgt
     1381 ccgcctgccc gcgctcctcg gtgcggcgtc ggccctgcgg gaccgcggcc gcggtcgccg
     1441 catcacgttc tcggccaagg tcttcatccc gctcaccacc ctctgccgcg actattgcgg
     1501 ctactgcacg ttccggaagg atcccggtga gccgggcgcc ttcacgatga cgcccgatca
     1561 ggtgctggcc ctggcccgcg cgggcgcgcg actcggcgcc aaggaggcgc tgttctcgct
     1621 gggcgacaag cccgaggcga tcttcccgga gcaccgtgcc ttcctgcgct cggtgggaca
     1681 ccgcacgacg ctcggctacc tgcgcgagat cagcgcccgc gtcctggatg agacgggcct
     1741 gctcccgcac gccaacccgg ggctgatgac ggagcgggat ctggcctcgt tgcgagaagt
     1801 caacgtgagc atgggcatca tgctcgagag cacgtccgag cggctgcttg ccccgggcgc
     1861 cgcgcacgac cgggcgcccg acaaggtccc gcagcggcgc ctccggacca tcgcgcgcgc
     1921 gggcgccctc ggcatcccct tcaccaccgg catcctcatc gggatcggcg agacccacgc
     1981 cgagcgggtc gactcgctcc tcgccatccg cgacctgcac gcacgccacg gccatatcca
     2041 ggaggtcatc atccagaact tccgcgccaa ggcgacgatt ccgatgcgcg gctggcccga
     2101 gcccggcgcg ctcgatctcc tccggaccgt cgcggtggcg cggctcctgc tggggccggg
     2161 catgaacatc caggccccgc ccaatctgtc ggcggacggc tacgatcagc tacccgccgc
     2221 cggcctcaac gactggggcg ggatctcgcc gctgaccccc gatcacatca atcccgagcg
     2281 cccctggccc ggcctggccg agctgcggcg ccgcaccgag gccgccggcc acgagctgcg
     2341 cgagcgcctc gccgtctatc ccgagtacac tacccgcgag gcgttcctcg acgagcgact
     2401 gcgctcgcgt gtggccgggc tcgtggacgc cgagggcttc gtccgacctg acctcgagat
     2461 ttggagacat tggtgatgcc gaccgacaag ccgacgacga tctcgctgga ctgggcttcg
     2521 cccgacaccc gccgcatcct gggcgcgctg ctcgacggcg ccgagctgtc ggtggccgac
     2581 ggcatccgcc tcaccgagac ccgggggcgc gacctgcagg ccctgacgct ggtagccgac
     2641 gagatgcggc ggcgccaggc gggcgacgta gtcacctacg ttgtcaaccg caacatcaac
     2701 ttcaccaacg tgtgcatcaa gcattgcacc ttctgcgcgt tcagccgcga ccaccgcgag
     2761 gaggagggct acttcctgcc gatggaggag gtggtccgcc gcgccatcga ggcgtgggag
     2821 atgggcgcca ccgaggtctg catccaggcg ggcctgcccc ccaagctcga cggccggtac
     2881 tacgtcgatc tctgtcgcgc gatcaaggcg gccctgcccg agatgcacct gcacgccttc
     2941 tcgcccgagg agatcctcta cggctccacc cgctcgggcc tgtccatccc ggagtacctg
     3001 gccgagctca agaaggtcgg gctcggaacg ctgcccggga cgtcggccga ggtgctggac
     3061 caggagatcc gggacgtcat cgcgcgcggc cgcatcacgg tcaagcagtg gatcgaggtg
     3121 atcaccgcgg ctcacgccca gggcatccgg acgacgtcga ccatcatgta cggacacgtg
     3181 gagcggccgg agcactgggt gcggcacatg gacctgctcc gcggtatcca gaaggagacg
     3241 ggcggcttca ccgagttcgt tcccctctcg ctgatccacc aggaggcgcc gatgtaccgc
     3301 cgcggcctcg tgcccggcgt ccgccagggc gccaccgggc tcgaggtcat ccggatgcac
     3361 gcgctggcgc gcctcttcct gggtccgctc ttccggaaca tccagtgctc gtgggtcaag
     3421 gaagggccca agctcgccca ggtcctgctc gcggcgggct gcaacgacat gggcggtacc
     3481 ctgatcaacg aatccatctc cacctccgcc ggcgcatcgt acggtcagct cgtgccgccg
     3541 gtcgagctgc gccggctcat ccgcgacgcg ggacgcgtcc cggcccagcg ctccaccacc
     3601 tacgagctgc tccgcgtcta cgaggtggaa gacccggcgg acgactcgcc gctcgatcac
     3661 gtggccgacg cggaggcgcg gttcggatcg tatcgggccc tggccggatc cgggcagttc
     3721 cgcttcctgc atcccacgcg cgcgccgggc gactaggaca tcggctccgc gcattcgccc
     3781 gcgcattcgg cagttgagcg cgcgggccgg cttgagtaga attggctctt ggctccacga
     3841 gacgtgtgaa tcctcaacgg cccgaccgtt cactctcaca tccaaacacg gagggaagca
     3901 catggcaaaa tcgatgacga agtccgccat catcgctcac ctggcccaga agacatcgct
     3961 cacgaagaag cagatcgtgg acgtcatgga ccagctcctg tcgctcgcga ccaaggaagc
     4021 gaagaacgtg ttcgtggtgc cgaacttcgg ccggctcgtg ctggccaacc gcaaggcccg
     4081 catggggcgc aacccgcaga ccggcgagcc catcaagatc ccggccaagc gcgtggtgaa
     4141 gttccggctg gccaagtccc tcaaggactc ggtgcttggc aagaagtaac acgcacggcg
     4201 ctctcgcata tcgaccgctc cccgcgtcct gggagcaggc ccgcgagctg ttccggttcg
     4261 cgggagcctg gaccccaccg ccggccgacg ttcacgccgg atggggtgcc tgggacgggg
     4321 agcgactcgt gggagcggtc ctgatggagc gggcgggcgc cacggcgatg ctgcacggac
     4381 ccgtggtcgt ggccccgccc gacacgccga ccgaggatgt gctggccgtg gcgaccgagc
     4441 tcgtgaacca ggccctggcg cacgccggcg ccgccggcat cgccaccgtc tttgcccggc
     4501 cccagagcct cgaccgggtc tgggtgcgca gcggcttcat cccgatcccc gaggcagagc
     4561 tgcctcgcga gctccagggg cgcccggggc tcgggctgtt cggctggcgc ggcggcaccg
     4621 cgctctggag caccgcggga cgcggcggct cccgctccgc cctggcccgc ggcgagcact
     4681 gacccgccct tccccgcgcc atgaagctca ccgcgctcgc gggcggcacc ggtgccgcca
     4741 agcggctccg cgggctctcc cggatcatgg acccgcgcga tctcaccgtc atcgtgaaca
     4801 cgggtgacga cgcggagatc tggggtctgc acatctcgcc ggatctcgac accgtctcct
     4861 acacgctggg cggcgtgatc gacgagcaga agggctgggg cctcaccggc gagacgttcc
     4921 acgccctcga ccagatcgcc cggttcggcg agccggtgtg gttcaacctg ggcgaccgcg
     4981 acctggccac tcacctgcac cgcacacggc tgctccggga aggccgcacg ctgaccgacg
     5041 tcacccgctc gatcgccgcg gctctcggcg tgggggcgac cgtgctcccg atgtccgacc
     5101 agccggtgcg cacgcggctc ctcggcccgg acggctggct gaccttccag gagtacttcg
     5161 tgcgggagaa ggcccaggtc gaggcgcgcg cggtggacta cgtgggcgcc gccgcggcgg
     5221 cgccggcgcc gggcgtggtg gaggcgatcg ccgccgcaga cgccgtcctg gtgtgcccat
     5281 ccaacccgat cacctcggtg ggcccgatcc tggccgtccc gggcctcgtg gaggcgctgg
     5341 gggccacgtc ggcgaccgtg gtggccgtca gccccatcgt gggtggcgcc gccgtctccg
     5401 ggccggccgg gcggctcatg gcctctgccg ggctgccggt ctcggccgcg ggcgtcgccc
     5461 gtgcctacgc cgcatggctc gaccgcctgg tctacgacga gcaggaccgg gccctggcgg
     5521 gcgagatccg agccctgggc gtcgcgcccg tgcccgcgcc caccatgatg tcgagccgcg
     5581 aagccgagat cgcgctggcc cgccacgtct tgagcgctgc cgcatgagcc tgctcctggc
     5641 cgtgcccgtg aaggacctgg tcaatgccaa gcagcgcctc attcccgcgc tgtccccatc
     5701 cgagcgtcgg gagctggcgc gcgccatgct ggaggatgtg ctcgacgcgg cggtggccgc
     5761 gctgccgggc gccgtgctcg tggtcaccac ggatccggag gtgcaggccg ttgcccgcgc
     5821 ggccggggcc ctgtgcctca ccgagagcgt caaccgcggc cacaccgccg cggtggcctt
     5881 cgcccagcga gaggccgtgg cccggggcgc ggcgcgcttc ctcacgctac cgggggacgt
     5941 gccctgcgtc accgcccagg agatcgccac actctgcggc gccctcgcgg aggcccccgg
     6001 cgtcgtcttc gtcccctcgc gctcgggcca gggtaccaac gcggttctcc tggccccgcc
     6061 cgatgcaatc ccgctcacat ttggcgagcc ctcattcgag aatcacttga agaccgcgcg
     6121 cgccgccgga ctaaccccgg aggtactcga gctgcccggc atcggcctcg acgtggacgc
     6181 gccggacgac ctgcccgtcc tcctggagcg cggacccacc acgcgaagcg cgcggctcct
     6241 gcgcggcttc ccggcggccg cgcgagccgg gggagcccgc tgagcatgcc gccgcgctac
     6301 gaggtcatcg gcgtacaggg gctgcccgag ttccgtccgg gggacgacgt cgcggccctg
     6361 atcgcggacg cggccggccg ccagggcacg ccgatcgtgg cgcgagacct cgtggtggtc
     6421 agccagaaga tcgtgtccaa ggccgaaggg cggctcgtgc gcctctcgga ggtgaccccc
     6481 tccccgcggg cccgggcggt cgcggaggag atcgggcgcg atccgcgcct ggtcgaggtc
     6541 atcctgagcg agagccggcg cgtcgtccgc caggccaagg gcgtgctcat cgtggagaca
     6601 catcacggct tcgtctgcgc gaacgcgggc gtggaccagt ccaacgtgga cgcggacacc
     6661 gcctgtctgc tgcccgagga ctcggatcgt tccgcggccg cgctgcgcga gcggctgcag
     6721 gcgctcaccg gccacgagct ggccgtgatc atcgccgaca ccttcggccg gccctggcgg
     6781 gagggcctcg tcaacgtggc ggtcgggctc gccggcttcg agcccatccg cagctatctc
     6841 ggcgagctgg atccggcggg ctacgtcctg caggccacca tcctggcgct ggccgacgag
     6901 ctggccgggg ccgccgagcc ggtgatgggc aagctcgacc gcattccggt ggcgatcatc
     6961 cgcggactgg actgggagcg gggcgacggg tcgagccgcg cgctgctccg cgatccggcc
     7021 cgcgacctgt tccgataacc cagagcgagg agacgtcatg cggatcggca tcgtgggcgg
     7081 caccggcaag gaaggcgtgg ggctcgggct gcgctgggcg caggccgggc acgaggtgat
     7141 catcggctct cgcgatccgg agcgggcgcg cgcgaaggcg gcggagctgg ccgcgtccac
     7201 tcccggcagt cgggtggacg gcatgtcgaa ccgggacgcg gccgccaccg ccgacgtgat
     7261 cgtcctgacc gtcccggcca cgggcctgca gaccacgctt cccgagctgc gcgacgtctg
     7321 tcgcggcaag atcgtggtga gcacggtggt acccctcacc ttcggagggg ggcgcctctt
     7381 cacgccgccg ccgcagggct ccgccgccga ggaagcgcaa gagatcctgg gccccgaggc
     7441 ccgcgtggtg gcctcgttcc atcacatcgc ggcccacgag ctggcggcgg gggaccacgc
     7501 gatcgagtgc gatctgctcc tgtgcggggg cgacgccccc gccaagaagc tggtgaccga
     7561 gctgggcgcg tccatgggcc tgcgcgcggt ggacgtgggc gcgctcacca acgcgggccc
     7621 gctcgagggg atcaccgcgg tcctcgccac gatcaaccgc cgctacaagc tgaagaactc
     7681 cggcatcaag atcaccgggt tgccctgaga gaggacccga ccatggccga cacccccatc
     7741 accgtctccg tcgccgccct gaccgcctac atcgtctcgg tgctggaagc gctcaagatg
     7801 tcgccgagga atgccgccct ctccgccgat ctcatggtcc gcaccgacct gcgcggggtg
     7861 gactcgcacg gcatcggcat gctcccgaag tacgtggagt ggacccgcgc cggcttcatc
     7921 caccccgacg cggagcccac ggtggcgcgc gacgacatgg ccaccgcgct ggtggatgga
     7981 cagaagggcc ttggccatcc cgcctccgtc ctggcgatgg agctggccat caccaaggcg
     8041 gcgacctacg gtatcggcat cgtcgcggtg cgcaactcga accatttcgg tgcgtgcgcg
     8101 aactattcca tgatggccct cgagcgcggg atgatcggct tcgcggtgac caactcgccc
     8161 ttcgtggcca tggtgccgac cttcgccagg aagccgatga tgggcaccaa ccctctcagc
     8221 ctggccgccc ccgccgcgcg gcacgccccg ttcgtgctcg acatggccac caccaccgtg
     8281 gcggtcggca agctcaccat cgcctcgcgc tggaacaagc cgatccccga gggctggggc
     8341 ctcgacgcgc atggccggcc gacctccgac gcgcgcgagg tgctcgcctc gcgcctgctc
     8401 tccccgctcg gcggcacccg cgagctgggc ggccacaagg gctatggcct gggcgtgatg
     8461 gtggacgtcc tctccggcgt gctctcgggc ggcgtctacg ggaacgtgct cgaccgcacc
     8521 aacggccgcg agcgcaagga gacgaacacc ggtcattgct tcgcggccat cgacatcaag
     8581 cgcttccggc ccctcgggga cttcaccgcc gcgatggacg acatgctgca ggccctccac
     8641 gacacgccgc gcgccgacgg ccaggagcgc atctacaccg cgggcgagcc cgaggccgag
     8701 accgagcgcc tgcgccgcac ccaggggatc ccggtggctc ccgccctcgt ccgccagtgc
     8761 aacgacatcg ccactgagct aggcgtcaag cccctcgcct gagcagcgcg gagtaagcgc
     8821 cggcacggtt gaccccggcc cgggtcgcac gaagcgcgac aatgggggag tgcgggggcc
     8881 attgctgggc ctccgcatta gaaagaaaaa cgggtcgccc tacggaagag ctatgcgcgc
     8941 acgtcccacg agaccaggac ccgtcgcctc tacctgccca gcacggccgc gagcaccttc
     9001 tccacatccc ccaggtcgtc gaagaggtgg tcggggcggt cggcgaggag ctcatcgcgt
     9061 gggtactggc cggtggtgac cgcgatcgcc accgcgccga aggcgcgcgc gcaatcgatg
     9121 tcgtacggcg tgtcgccgat cacgaccacc tcggggggca cgaaggcgta gcccacgagc
     9181 gcgtgagccc gccgcgtcgc cagcgacggc aggtgccgcc gatccatgtg gtcggagccg
     9241 tacgccccga cgcggaagtg cggccagaga cccgtgggca ggagcttgat ccgagcgccc
     9301 tcctcgatat tgccggtcac caggcccagc accacgtcgg ggcactctgc caggcgcgcc
     9361 accaccgcgg acacgcccgg cagcgtgacc acgtcgcggc cgtcaccgat ctcgctcgtg
     9421 agcccgccca cgtaggcctc gaagaagtcg tcgaggcgct ccttcacccg tgcaggctcc
     9481 cagcccagcg cacccatcac gtcgtggacg atgcgggtgt cggtccggcc gcgcaggtcg
     9541 tactggtcga tgccccccgc ggcgccgaag acccgctcga gcgcggtccg catcgcgcgc
     9601 cgccccgccc cgcgcgaggt gatcagggta ccgtcgatgt cgaacaggac gagcttcacg
     9661 gtgcgaggcg ggcgggctag gtgtggagcg cggcggcgag cgcgcgcagg cgcgccacga
     9721 tgggcggtag ccggtccagc accgcttcca cgtcggccgc ggtgctccac cggccgagcg
     9781 tgaagcagag cgagccttcc gcctccccgg gtgcacaccc gatggcccgc agcacgtgcg
     9841 acgtcgtctg ggtcagcgac gcacaggccg agcccgacga ggcggccacg ccgtccagat
     9901 cgaggtccag cagcacgctg tcggccttca cgccgcgtac cacgaggctc aggtggtgcg
     9961 gcaaccgcga ggtcgccgac ccggtcagcc ggcagtccgg gatcgcctcg agcagcccgt
    10021 gcatgagccg gtcgcgcagg gtggcgagcc gtgtcgcttc ctggtgggca ccctcgacgc
    10081 gcatcagctc ggcggccacc ccgaggccga ccaccccggg cacgttctcc gtgcccgagc
    10141 ggtacccgcc ctcctggccg ccccccagca cctgtgggtg gatccgcacg ccgggccgga
    10201 cccagagggc gccgctcccc ggtggagcat agaggtcgtt ccccgagacg gtcagcaggt
    10261 cgatgctcat cgcctccacg ttgagcggca cccggccgag cgcgccgacg ccgtccacgt
    10321 gcagcgccac gcccgcggcg cgcgtgatcc gcccgatctc cttgagcggt tgcacggtgc
    10381 cgatctctgg gttggcggcg gcgatcgaga cgagcacggt atccgcggtg agggccgccc
    10441 tgagcatgcc cggatccacg cggccctcgc cgtccaccgg caggtagccc acggtcacgc
    10501 ccgccttctc gaggtcgcgg aaggcgttga tcaccgagat gtgctcgatg gccgagagca
    10561 ggacgcggac gggccccccg ccccgcgcgc gggccaccac gcccttgatc gccaggttgt
    10621 tggcctcggt ggcgccggag gtgaacacga tgccgcccgg cgcgccgccg cagaggcgcg
    10681 cgaccttgac gcgggccgcg tcgagcgact cgcgcgcctc cgcgcccagc gagtgacggg
    10741 ccgagggatt gccgataccc gcttccagga acgggcgcgt gaccgcgagg acgcgcggat
    10801 ccacgggcgc gaagcccgcg tagtcgaggt agacgcgtcc gggctccacc ccggcgggct
    10861 ccaccggcat ctaccgtgtc ttcctgatca cgaagcggta gaccgccccg ttccgggtga
    10921 cctcgagcag ctcgtggccc gtgttctgcg cccagctgcg catgtcgggg tcggaggcgg
    10981 gatcgtcgga cagcatctcg aggacttgac ctctcgccat ctgggtcatc gcctcgcggg
    11041 tcttgacgat gggcatcggg cagaacagcc cagtgcagtc aatctgcctg tcaggattga
    11101 cgacttttgc ttccagggcc atggcaggtt accgtacccc agccttccgc accccgtcaa
    11161 ggaagggcgc ggcgctgaaa ttctcgccgc attgaagggc cgtcgccgct tatccttggg
    11221 gttgacgcca tggccagacg cgccagagct gcccagaccc ccgcggcccg ccgcatcaag
    11281 cgcaagatcc tgatcgcgat cgccctgacc gcgttcctgc ccatgctggt ggtcgcctat
    11341 ctcgtctacg cgtatgcgct cccgcttctg gacccgcagg cccaggagcg caacctgccg
    11401 tggatgctgg tcatgttggg cgggaccggg ctcctcatgg cggcaggcag cttcatgctc
    11461 tgggacctgg ccacctcggt ggtacgccac gcggactcgc tcgatgcgcc gcgccggccc
    11521 gctccggccg cgggcgcgtc ccggcccgcg gcgagccccg agaccaccgc ggtgcagcag
    11581 tacgtgcagc agagcgacga ggtggactcg ctcatgcagt ccttctcgcg gatgctggtc
    11641 accatcgagc agcaggcctc cgacgtcaac gaagacgcgc gccggctcga caccgcctac
    11701 aaggagctcg agtccaccag cgcccagctc aaggagttct cgttcaagga cgaggtcacc
    11761 ggcctctaca accgccgctt cttctcgatc cggctcgagg aagaagtctc gcgct
//
LOCUS       DASUFA010000363         4938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_92062, whole genome shotgun sequence.
ACCESSION   DASUFA010000363 DASUFA010000000
VERSION     DASUFA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92062"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFX87_13330"
     CDS             <1..188
                     /locus_tag="VFX87_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995957.1"
                     /translation="AVVGLGAVAAVMLAAVATPLGVALRRATLPMVVIAAALGAVLTV
                     AGVIALVAREGSRDPES"
     gene            complement(216..1634)
                     /locus_tag="VFX87_13335"
     CDS             complement(216..1634)
                     /locus_tag="VFX87_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4995958.1"
                     /translation="MTSIDPVWAGVAQLSQAFGARTLSPVDAVDALLDRIRRHDGRLH
                     AFVSVYADQARLAAEGADRAIRSGHRIGPLHGVPIALKDLVDLEGRVTTGGSKVWIAR
                     VSPITATLAERAIAAGMIVLGKTHTVEFAMGSWGTNTHMGTPWNPWDAAVHRTPGGSS
                     SGSGVAVSAGLAPVAIGTDTGGSVRLPAAWCGIVGLKVTAGRISTFGVLPLSFTLDTP
                     GPLARSVEDAALIFRLLNGPDPRDPQTLHWAPADPMPTLRRGAAGLRLAVMPDVERAG
                     VDKDMLAAYDASVETLAKLGARIERVKLPHRFSDYAAATGRIIGAEGYRFVGHLVDDE
                     RLPVDPHVRPRIQIGRGISARDYLLALAEREERSREVAAALSHVDALLTPTVLTPPIP
                     IEQADQAGTAAHFTRPVNYLGLCGLAVPNGFTAGGLPTSLQIVAHAGDEAMALRIGQA
                     YEEAAGWKTRRPPEPAATERAR"
     gene            complement(1631..2863)
                     /locus_tag="VFX87_13340"
     CDS             complement(1631..2863)
                     /locus_tag="VFX87_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995959.1"
                     /translation="MRTVLANATVIDCVTPRPVAGASVVIERGRIVELLGAGRSPDTR
                     DARVVDLEGAYLLPGLWDVHIHPDYLASTGASVAEQTAIFGHRLMQALTESGVVGVRC
                     AGAAHFMDVAWKRAFEAGQYTGPRVFAAGYFLTTTGGHFLTSGHAHECDGPYGFVRAI
                     REQIKNGVDHIKLNLTGGIMGPAWDRHSQAFLLNDELEAAFAICRQRGFRVMAHAASP
                     ETVQAAARLGAHSVEHGYIMDGPAVESLRAHRTWYVPTLAITHLTPDQARDPWEKRWV
                     EERNLAPDLCHRADAAAGPHREAFRQALAAGVRMALGSDIRPLRDAALLELGLWVKAG
                     ATPWQALLAATRHAAELCGVGDELGTIEPGKLADLIVVAANPLENITNLRRLLLVLKD
                     GSIVSDKRGPNQHGGLSE"
     gene            2953..3825
                     /locus_tag="VFX87_13345"
     CDS             2953..3825
                     /locus_tag="VFX87_13345"
                     /inference="COORDINATES: protein motif:HMM:NF020127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4995960.1"
                     /translation="MPRPPGVPVSLVSVSTRDGVVLEGVAVHPRGRRRTALVWVHGLG
                     STFSSGQPLIRVLSARLNAAGIGYLKLNTRGHGVVTRAGRRLAGAAFERFAESVHDLR
                     AAVALAIRSGYRRVILAGHSTGANKVLHYMARTRDRRVAGLILLGPVSDVAGERKRIG
                     RRELARRVAAAERLARRDPEALVPRAWGFRGAARFVSLYRPGEAEDVFPYYRPDADWR
                     ALGRIRVPTAVIVGSRDEYLDRRAAELVAAFERHATGARAFTGIIVPRARHGFRGHEP
                     ALARAVVRWARTVR"
     gene            complement(3864..4607)
                     /locus_tag="VFX87_13350"
     CDS             complement(3864..4607)
                     /locus_tag="VFX87_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX4995961.1"
                     /translation="MPNSDRKLEIVTEVPESAMVVFAHPDDAEIGSGGVIATWVAAGC
                     EVSYVLCTNGDAGTADRDLSPAELAHKRAHEQRAAADFMGVRHVEMLGYPDGRLEDTR
                     ELLGDVVRAIRRHRPHTVFVHDPYRMKGFQHRDHRKAGIATTDAVYPYARDHLHFAEH
                     ITKEGLEPHKVRELWYWGADEPDVIVDVTAGIERQIAALVRHQSQMPGFNVPEGVTIG
                     ERVKKGAAQLGADYGFEYGAAFRRLVARR"
     gene            complement(4626..>4938)
                     /locus_tag="VFX87_13355"
     CDS             complement(4626..>4938)
                     /locus_tag="VFX87_13355"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX4995962.1"
                     /translation="APALARTVAAVFARRGFEVQENVSGYTGGNIVATYGQPRAGVHA
                     LQLEINASLLMTTSREEFIAHISRGGIPEKAEANIARVRRCLQEVMAALPAVLATVHE
                     P"
BASE COUNT          613 a         1728 c         1899 g          698 t
ORIGIN      
        1 tggcggtggt gggcctcggc gcggtcgccg cggtcatgct cgccgcggtg gcgacgccgc
       61 tcggcgtcgc gctccggcgg gccacgctgc cgatggtggt gatcgcggcg gcgctcggcg
      121 ccgtcctgac ggtggcgggt gtcatcgcgc tggtggcgag agagggaagc cgcgatccgg
      181 agtcctgacc gacgcaaccg cgcggcgttt ctaggtcagc gcgcccgctc cgtcgctgca
      241 ggctccggag gccgccgggt cttccagccg gcggcctcct cgtaagcctg gccgatccgc
      301 agggccatgg cctcgtcgcc cgcgtgcgcc acgatctgca gtgatgtcgg caggccgccc
      361 gcggtgaagc cgttgggcac cgcgagcccg cagaggccca gatagttgac cggccgggtg
      421 aaatgggccg cggtgccggc ctggtccgcc tgctcgatcg ggatgggcgg cgtcagcacg
      481 gtcggggtga gcagcgcgtc cacgtgagac agcgcggccg ccacctcgcg gctccgctct
      541 tcacgctcgg ccagcgcgag cagatagtcg cgcgcggaga tcccgcggcc gatctggatg
      601 cgcggccgca cgtgcggatc cacgggcagg cgctcgtcgt ccaccaggtg cccgacgaaa
      661 cggtagccct cggcgccgat gatgcggccg gtggccgccg cgtagtcgct gaagcgatgg
      721 gggagcttga cgcgctcgat gcgcgcgccg agcttcgcga gggtctccac cgaggcgtcg
      781 taggcggcga gcatgtcctt gtccacgccc gcccgctcga cgtccggcat caccgcgagg
      841 cggaggcccg cggcgccgcg gcgcagggtc ggcatcggat cggcgggcgc ccagtggagc
      901 gtctgcggat cacgcgggtc cggcccgttg agcagccgga agatcagggc ggcgtcctcg
      961 accgagcgcg cgagggggcc cggggtgtcc agagtgaagg agaggggcag gacgccgaag
     1021 gtgctgatgc gcccggcggt caccttcagg cccacgatcc cgcaccacgc ggcgggcagg
     1081 cgcaccgagc cgccggtgtc ggtgccgatg gcgacgggcg ccagccccgc gctcaccgcg
     1141 acgccggagc cgctggagga gccgcccggc gtgcggtgca ccgccgcgtc ccacgggttc
     1201 cagggcgtgc ccatgtgggt gttggtaccc cagctcccca tcgcgaactc gacggtgtgg
     1261 gtcttgccga gcacgatcat gcccgccgcg atggcccgct cggcgagcgt ggcggtgatc
     1321 ggggagacac gcgcgatcca gaccttcgag ccgccggtgg tgacgcggcc ctcgagatcc
     1381 acgagatcct tcagcgcgat gggcacgccg tggagcggcc cgatacggtg gccggagcgg
     1441 atcgcgcggt cggcaccctc tgcggccagg cgcgcctggt cggcgtagac cgagacgaag
     1501 gcgtgcaggc gcccgtcgtg gcggcggatg cggtcgagca gcgcgtccac cgcgtccacg
     1561 ggagacagcg tgcgcgcgcc gaaggcctgc gagagctggg cgacgcccgc ccacaccgga
     1621 tcgatgctgg tcattccgag agtcctccat gctggttggg cccgcgcttg tccgacacga
     1681 tgctcccgtc cttcaggacg aggagcagcc ggcgcaggtt ggtgatgttc tcgagcgggt
     1741 tggcggccac cacgatgagg tccgcgagct tcccgggctc gatggtgccg agctcgtcgc
     1801 cgaccccgca cagctccgca gcgtgacggg tggcggcgag cagtgcctgc catggcgtgg
     1861 cccccgcctt cacccagagg cccagctcga gcagggcggc gtccctgagg ggccggatat
     1921 ccgacccgag cgccatccgg acaccggcgg cgagcgcctg ccggaaggcc tcgcgatgcg
     1981 gcccggccgc cgcgtccgcg cggtggcaga ggtcgggcgc gagattgcgc tcctcgaccc
     2041 agcgcttctc ccacggatcc cgtgcctgat ccggcgtgag gtgggtgatg gccagggtcg
     2101 gcacgtacca ggtgcggtgg gcgcgcaggc tctcgacggc gggcccgtcc atgatgtagc
     2161 cgtgctccac gctgtgggcg cccagccgtg ccgcggcctg gacggtctcc gggctggccg
     2221 cgtgcgccat cacgcggaac ccacgctggc ggcagatcgc gaaggcggcc tcgagctcgt
     2281 cgttcagcag gaacgcctgg gagtggcgat cccacgccgg gcccatgatg ccgccggtga
     2341 ggttcagctt gatgtggtcc acgccgttct tgatctgctc ccggatggcg cggacgaagc
     2401 cgtacggtcc gtcgcactcg tgcgcgtgcc cggacgtgag gaagtgcccg ccggtcgtgg
     2461 tgaggaagta gccggccgcg aagacccgtg ggcccgtgta ctggcccgcc tcgaaggccc
     2521 gcttccacgc cacgtccatg aagtgcgcgg cgcccgcgca ccgcacgccg accacgccgc
     2581 tctcggtcag ggcctgcatc aggcggtggc cgaagatggc ggtctgctcc gcgacggagg
     2641 cgcccgtgga agcgaggtag tcggggtgga tgtggacgtc ccagaggccg ggcaggaggt
     2701 aggcgccctc gaggtcgacg acccgggcgt cgcgcgtgtc gggggagcgc ccggccccca
     2761 gcagctccac gatgcggccg cgctcgatga cgacgctggc gccggcgacc gggcgcgggg
     2821 tgacgcagtc gatcaccgtc gcgttcgcga gcacggttcg catcgcgccg atcctgccag
     2881 agccccgggg gcaggtcaaa ccggccgcgg cgggtacttg acggccggga ggaaactggc
     2941 catggtgcgg gaatgccgcg gccgcccggc gtgcccgtgt ctctcgtcag cgtcagcacg
     3001 cgcgacggcg tcgtcctgga gggtgtggcc gtccacccgc gcggccgccg gcggaccgcg
     3061 ctcgtctggg tgcacggcct gggctcgacg ttctcctcgg ggcagccgct catccgggtg
     3121 ctgtcggcgc gcctcaatgc cgcgggcatc ggctacctca agctcaacac gcgcggccac
     3181 ggcgtggtga cgcgcgccgg caggcggctc gcgggcgcgg ccttcgagcg cttcgccgag
     3241 agcgtccacg acctccgggc cgcggtcgcg ctggcgatcc ggagcggcta ccgccgggtg
     3301 atcctcgcgg gccactcgac gggcgccaac aaggtgctcc actacatggc gcggacccgc
     3361 gaccgacgcg tggccgggct gatcctgctc gggcccgtct ccgatgtcgc gggtgagcgg
     3421 aagcggatcg gtcgccgcga gctcgcccgt cgtgtggccg cggcggagcg gctcgcccgg
     3481 cgggatccgg aggcgctcgt cccgcgcgcc tggggctttc ggggcgccgc gcgcttcgtc
     3541 agcctctacc ggccgggcga ggcggaggac gtcttcccgt actaccggcc cgacgcggac
     3601 tggcgcgcgc tcggacggat ccgggtgccg accgcggtga tcgtcggcag ccgggacgag
     3661 tatctcgacc ggcgggcggc ggagctggtc gccgccttcg agcggcacgc gacgggcgcg
     3721 cgggcgttca ccggcatcat cgtcccccgc gcgcggcacg ggttccgcgg gcacgagccc
     3781 gcgctcgcgc gcgcggtcgt ccgctgggcg cggacggtgc ggtaacgtcg ggtggatgcg
     3841 cggatcggcg ccctggccgg ctcctaccgc cgcgcgacca ggcggcggaa cgccgcgccg
     3901 tactcgaagc cgtagtcggc ccccagctgg gccgcgccct tcttcacgcg ctcgccgatg
     3961 gtcacaccct ccggcacgtt gaagcccggc atctggctct ggtggcgcac cagcgcggcg
     4021 atctgccgct cgatcccggc ggtgacgtcc acgatgacgt cgggctcgtc ggcgccccag
     4081 taccagagct cgcgcacctt gtgtggctcg agcccttcct tcgtgatgtg ctcggcgaag
     4141 tgcaggtggt cgcgggcgta ggggtagacc gcgtcggtgg tggcgatgcc ggccttgcgg
     4201 tggtcacggt gctggaagcc cttcatccgg taggggtcgt ggacgaagac cgtgtgcggc
     4261 cggtgacggc ggatggcccg gaccacgtcg cccaggagct cgcgcgtgtc ctccagccgg
     4321 ccgtcggggt agcccagcat ttccacgtgg cgcacgccca tgaagtcggc ggcggctcgc
     4381 tgctcgtggg cgcgcttgtg ggccagctcg gcgggggaga gatcgcggtc ggcggtcccg
     4441 gcatccccgt tggtgcacag cacgtagctg acctcgcacc cggcggccac ccaggtggcg
     4501 atgaccccgc ccgagccgat ctcggcgtcg tcgggatggg cgaagaccac catggcggac
     4561 tcgggtacct cggtgacgat ctcgagcttg cggtcggagt tgggcatgga ggtgatctta
     4621 ccctgttatg gctcgtgaac cgtcgccagg acggcgggga gggcggccat gacctcttgg
     4681 agacagcggc gcacgcgcgc gatgttcgcc tccgccttct ccgggatccc cccgcgtgag
     4741 atatgggcga tgaactcctc gcgactggtc gtcatcagca gcgatgcgtt gatctcgagc
     4801 tggagcgcgt ggacgccggc gcgcggctgg ccgtacgtcg ccacgatgtt gccgccggtg
     4861 tagccgctga cgttctcctg cacctcgaag ccgcgacggg cgaagacggc ggccacggtc
     4921 cgggccaggg ccggggcg
//
LOCUS       DASUFA010000364         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1341130, whole genome shotgun sequence.
ACCESSION   DASUFA010000364 DASUFA010000000
VERSION     DASUFA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341130"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFX87_13360"
     CDS             complement(<1..207)
                     /locus_tag="VFX87_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4995963.1"
                     /translation="MPLFDTHAHLHFPELLADLDAVLERARAAGVTGMVTIGTDRETN
                     PAAVALAERLHDVHATVGFHPHDAG"
     gene            complement(230..1249)
                     /locus_tag="VFX87_13365"
     CDS             complement(230..1249)
                     /locus_tag="VFX87_13365"
                     /inference="COORDINATES: protein motif:HMM:NF039296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HEX4995964.1"
                     /translation="MTDLRSAPAPRLPLVGWLGGWPISIASLVLGLAVLFVFRRGLPH
                     VGWIVGYLLLLWLLFVLITELRAPLTERGRGLVLSAADYTIQTLYHNLLLFVLPAYYA
                     AATLDSPNVVFPVLVAAGALVTALDPVYRRVVSPRPWLGHALLGFSVFAALNVALPLV
                     GVQPILALEASAVMAALALAPAFRREGFLGWLRAHALALAAASLALVLVWFARGLVPP
                     APLFLARAVGARGVAELEPVEPVEGGVVAAATVLEWGELAAYTAVYAPAGVRQPIAHV
                     WWRDRQRLARVALPTPVLGGRQQGFRTYSRFTDLKPPVAGRYQVDVVTASGQLIGRLR
                     FTVTP"
     gene            complement(1246..2001)
                     /locus_tag="VFX87_13370"
     CDS             complement(1246..2001)
                     /locus_tag="VFX87_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995965.1"
                     /translation="MRGVLGETFRLLVRHLHLFTLISLTVWLPTHVLLNYLEFFDPGE
                     DSAGRALRIVLTAQVVLDPFVVSAQLSALARIKQGVPVTYWQALAEGFSAWPRLMLVR
                     FVVIWVLLLPSLGWLAIYQAGPALRLVGSLALIGLTMLILALLVRFALVDSAVVLGGA
                     SPFNAWRQAAELTAGQRWSILWTAATLFVLIFGVAVAGGQAFRTVPGANHFVARVLFD
                     CMLAVAQSLFTIALFLFYWRKQGIPAPAEPART"
     gene            complement(2001..3938)
                     /gene="metG"
                     /locus_tag="VFX87_13375"
     CDS             complement(2001..3938)
                     /gene="metG"
                     /locus_tag="VFX87_13375"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX4995966.1"
                     /translation="MSTFYLTTPIYYVNARPHLGHACTTIMADAMCRYRRLAGDRVYF
                     LTGTDEHGERIAQVAAAAGKSPQAYADEIAAVFRETWQRLGITNDDFIRTTEPRHRKV
                     VQEILQRLHDAGEIYFGEYGGWYCYGCERFYTEKEIVDGKCPDHQTALTFVKEKNYFF
                     KMSKYQAWLIKHLEDNPGFIQPERYRNEVLGFLREPLQDLSISRPRSRLEWGIPLPFD
                     DQYVTYVWFDALINYYSAVSDPSNAKTRGLWEHVEHLIAKDILKPHGVYWPTMLKAAG
                     IPLYRRLNVHGYWSLGGSKMSKSVGNVVEALQLTDKYGHDAFRYFLLREMNFGLDASF
                     SEEAFVDRLNADLANDLGNLASRATTLIAAAGPVAQVTGPVEDADREIATAAAEARAV
                     IEQAMQDFAFQKALGGIWTFIGSVNRYVDTSAPWALAKDPAKRPRLERVLCTLADSLG
                     FLGTVLDPFLPDAARKIREALGQSAPPTLAAAVVGRLTAVPRVTKISGLFPRIDTKAP
                     HPGPLPGGERESSTLSPPGRGQGEGPKAEGARIPITDFQKVELRVAEVLAAERVPKSK
                     KLLKLTIRVGEETRTLVAGVAEHYEPGDLVGRKVVIVANLQPATLMGVESNGMVLAAE
                     HGGTLSLLTLDRDLPSGAKVK"
     gene            complement(3958..>4543)
                     /gene="ricT"
                     /locus_tag="VFX87_13380"
     CDS             complement(3958..>4543)
                     /gene="ricT"
                     /locus_tag="VFX87_13380"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX4995967.1"
                     /translation="GVETCQRMARGRALAMKIVDVEIEASRRRVTVFFNSEERVDFRD
                     LVRDLAREFHARIEMRQIGARDTTKMLDGIGPCGRQLCCSSHLRKFDPISVKMAKAQD
                     MPLTDSRLLGNCGRLKCCLLYEFSTYEALRSRLPRVNTACSADCGGGGCMTGKVRSLR
                     VLKQSVVVGFPDGTEAEVPLDHLTWEGRDHIKPA"
BASE COUNT          731 a         1558 c         1650 g          604 t
ORIGIN      
        1 cccggcgtcg tggggatgga agcccacggt ggcgtgcacg tcatgcagtc gctcggccag
       61 cgccaccgcg gcgggattgg tctcgcgatc ggtgccgatg gtcaccatcc cggtcacccc
      121 ggcggcacgc gcgcgctcca gcaccgcgtc gaggtccgcc agcagctcgg ggaagtgcag
      181 atgcgcgtgc gtgtcgaaga gcggcatcag tcggtccggc gcaatgatat cagggggtga
      241 cggtgaagcg caggcggccg atgagctggc cggacgccgt caccacgtcc acctgatagc
      301 gacccgcgac gggtggcttg agatccgtga agcgggaata ggtgcggaag ccctgctgcc
      361 ggccgcccag caccggcgtc ggtagggcca cgcgggcgag ccgctgccga tcccgccacc
      421 agacgtgggc gatcggctgc cgcacgccgg ccggcgcata gaccgcggtg taggcggcca
      481 gctcgcccca ctcgagcacg gtggctgcgg ccaccacgcc gccctccacc ggctccaccg
      541 gctccagctc ggccacgccg cgcgcgccga ccgcgcgggc caggaagagc ggcgcgggcg
      601 gcacgagccc gcgcgcgaac cagaccagga ccagggcgag cgaggccgca gccagcgcca
      661 gggcgtgggc ccgcagccag ccgaggaacc cttcgcgccg gaaggcgggc gcgagcgcga
      721 gcgcggccat caccgcgctg gcctcgagcg ccaggatggg ctggacgccg accagcggca
      781 gcgcgacgtt gagcgcggcg aagacggaga accccagcag cgcgtggccg agccagggcc
      841 gcggggacac gacccggcgg tagacggggt cgagcgcggt gacgagcgcg ccggccgcca
      901 cgagcaccgg gaacaccacg ttcggcgagt cgagcgtggc cgccgcgtag taggcgggga
      961 gcacgaagag cagcaggtta tggtagagcg tctggatcgt gtagtcggcc gcggagagca
     1021 cgaggccccg cccccgctcg gtgagcggcg cgcggagctc ggtgatcagc acgaagagca
     1081 gccagaggag cagcaggtag cccacgatcc agcccacgtg gggcagcccg cggcggaaca
     1141 cgaagagcac cgccaggccc aggaccagcg aggcgatgga gatcggccag cctccgagcc
     1201 agccgaccag cggtaggcgc ggcgcgggcg cagagcgaag gtccgtcacg tccgggcggg
     1261 ctcggccggc gcgggaatcc cctgctttcg ccagtagaag aggaagagcg cgatcgtgaa
     1321 gagcgactgc gccaccgcca gcatgcagtc gaagagcacc cgagccacga agtggttggc
     1381 gccgggtacc gtccggaagg cctggccgcc cgcgaccgcg accccgaaga tcagcacgaa
     1441 gagcgtggcg gcggtccaga gaatgctcca gcgctggccc gcggtgagct cggcggcctg
     1501 ccgccaggca ttgaacggcg acgccccgcc cagcaccacc gcggagtcca cgagcgcgaa
     1561 gcgcacgagg agcgcgagga tcagcatggt cagcccgatg agggccaggc tccccacgag
     1621 ccggagcgcg ggccccgcct ggtagatggc cagccagccg agcgacggga gcagcagcac
     1681 ccagatgacg acgaagcgca ccagcatgag gcggggccag gcgctgaagc cctcggcgag
     1741 agcctgccag taggtgaccg gcacgccctg cttgatgcgc gccagcgcgg acagctgcgc
     1801 gctcaccacg aacggatcga gcaccacctg cgcggtcaga acgatacgga gcgcgcggcc
     1861 ggccgagtcc tcgcccgggt cgaagaactc gagatagttg agcaggacgt gggtgggcag
     1921 ccacaccgtc agcgagatca gcgtgaagag gtgcaggtgg cggacgagga ggcggaacgt
     1981 ctccccgagc acgccgcgca ctacttgacc ttggcgccgg agggcaggtc cctgtcgaga
     2041 gtgagcagcg acagcgtgcc accgtgctcg gcggcgagca ccatcccgtt cgactccacc
     2101 cccatgagcg tggccggctg caggttggcc acgatgacca ccttccggcc gacgagatcg
     2161 ccgggctcgt agtgctccgc cactcccgcc accagcgtgc gggtctcctc gcccacccgg
     2221 atcgtgagct tgagcagctt cttggacttc ggcacccgct cggccgcgag cacctcggcc
     2281 acccgtagct ccaccttctg gaagtcggtg atcgggatcc tggccccctc agccttaggc
     2341 ccctcaccct gccctctccc cggaggggag agggtgctcg actccctctc ccctccgggg
     2401 agagggccgg ggtgaggggc cttggtgtcg atgcgcggga agaggccgga gatcttggtc
     2461 acccgcggca ccgcggtcag gcggcccacc accgcggccg cgagcgtggg cggcgcgctc
     2521 tgcccgagcg cctctcggat cttgcgggcc gcgtcgggca ggaagggatc gagcacggtg
     2581 ccgaggaagc cgagcgagtc ggccagcgtg cacagcacgc gctcgagccg gggccgcttg
     2641 gcgggatcct tggccagcgc ccacggggcg ctcgtgtcca cgtagcggtt cacgctgccg
     2701 atgaaggtcc agatgccgcc gagcgccttc tggaacgcga agtcctgcat cgcctgctcg
     2761 atcacggccc gcgcctccgc ggcggcggtc gcgatctcgc ggtcggcgtc ctccaccggc
     2821 ccggtcacct gcgccacggg gccggcggcg gcgatcaggg tggtcgcgcg gctcgccagg
     2881 ttgcccaggt cgttggccag atccgcgttg aggcgatcca cgaaggcctc ctcgctgaag
     2941 ctggcgtcga ggccgaagtt catctcgcgc aggaggaagt agcggaaggc gtcgtggccg
     3001 tacttgtcgg tgagctggag ggcctccacc acgttgccga ccgacttgga catcttgctc
     3061 ccgccgagcg accaatagcc gtggacgttg agccgccggt agagcgggat gcccgccgcc
     3121 ttcagcatgg tcggccagta gaccccgtgc ggcttcagga tgtccttggc gatcaggtgc
     3181 tcgacgtgct cccagagccc gcgcgtcttc gcattgctcg ggtccgagac cgccgagtag
     3241 tagttgatca gcgcgtcgaa ccagacatag gtcacgtact ggtcgtcgaa gggcagcggg
     3301 atgccccact cgaggcgcga gcggggccgg ctgatcgaca gatcctggag cggctcgcgc
     3361 aggaagccga ggacctcgtt ccggtaccgc tcgggctgga tgaacccggg gttgtcctcc
     3421 aggtgcttga tcagccacgc ctggtacttc gacatcttga agaagtagtt cttctccttg
     3481 acgaaggtga gcgcggtctg gtgatcgggg cacttgccgt ccacgatctc cttctcggtg
     3541 tagaagcgct cgcagccgta gcagtaccag ccgccgtact cgccgaagta gatctcgccc
     3601 gcgtcgtgga ggcgctgcag gatctcctgc accactttcc ggtgccgcgg ctcggtggtg
     3661 cggatgaagt cgtcgttggt gatgcccagg cgctgccacg tctcgcggaa gaccgccgcg
     3721 atctcgtccg cgtaggcctg cggcgacttc ccggccgcgg ccgccacctg ggcgatccgc
     3781 tcgccgtgct cgtcggtgcc ggtgaggaag tacacgcggt cgccggccag ccggcggtac
     3841 cggcacatcg cgtcggccat gatggtggtg cacgcgtgcc cgaggtgcgg gcgcgcgttg
     3901 acgtagtaga tcggggtcgt gaggtagaag gtgctcatgc gccgggtggg ccggccctca
     3961 ggccggcttg atgtggtcgc gcccttccca ggtgagatgg tcgagcggga cttccgcctc
     4021 ggtgccgtcc gggaagccga ccaccaccga ctgcttcagc acccggagag accgcacctt
     4081 gccggtcatg cagccgcccc cgccgcaatc ggcggagcag gcggtgttca cgcgcggcag
     4141 ccgcgaccgc agcgcctcgt aggtcgagaa ctcgtagagc aggcagcact tgagccgccc
     4201 gcaattgccg aggagccggc tatcggtcag cggcatgtcc tgagccttgg ccatcttcac
     4261 cgagatcgga tcgaacttgc gcaggtgcga cgagcagcag agctgccggc cgcaggggcc
     4321 gatgccgtcg agcatcttgg tggtgtcgcg cgccccgatc tgtcgcatct cgatccgcgc
     4381 gtggaactcg cgggccaggt cgcgcacgag gtcgcggaaa tccacccgct cctcggagtt
     4441 gaagaagacg gtgacgcggc ggcgcgaggc ctcgatctcg acgtccacga tcttcatggc
     4501 gagcgcgcgc ccgcgcgcca tccgctggca cgtctccacg ccg
//
LOCUS       DASUFA010000365        10297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_524251, whole genome shotgun sequence.
ACCESSION   DASUFA010000365 DASUFA010000000
VERSION     DASUFA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524251"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFX87_13385"
     CDS             complement(<1..611)
                     /locus_tag="VFX87_13385"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX4995968.1"
                     /translation="MGFDYGKYFGEIKLTHDLLSKKEERTWLLKPPRDGEHHDVVLYL
                     GCNVLRTSHLVETVGAVFDRLGLDYVAAGGPTYCCGIQHHRRGQEASGERYANHTVEM
                     LQSMAPREVVMWCPSCIQFYDEVLQAELPFPKRHTAEFLADCLERGLFTFTQRVEATV
                     ALHYHYATEARAREGRAARRLLEAVPGITVIEFEPDARWGRTCT"
     gene            694..1167
                     /locus_tag="VFX87_13390"
     CDS             694..1167
                     /locus_tag="VFX87_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX4995969.1"
                     /translation="MNTPIRTIAMLGLLVLATACSKAVLAPEPGASGVPAEGVEPSRN
                     ATAAHLATRYLGAPYVWAGSSPSGFDCSGLVMYAFGKVGVAIPHNAAQQFRLGTPVPR
                     NRLMPGDVVFFNQLRHNGIYLGDGRFVHATKPGGVKIARLDDDWFRTRWVGARRL"
     gene            complement(1184..1372)
                     /locus_tag="VFX87_13395"
     CDS             complement(1184..1372)
                     /locus_tag="VFX87_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEX4995970.1"
                     /translation="MAPVTYDCAFCGEENDVFADDSGGRHQTFTEDCGVCCRPNLITL
                     RWDDDGDVQIEAVQEYEA"
     gene            1417..2184
                     /locus_tag="VFX87_13400"
     CDS             1417..2184
                     /locus_tag="VFX87_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4995971.1"
                     /translation="MTRFQGKVALISAAGAGIGRATAMIVAREGGTVVGVDLEPALLD
                     RLTADVVAAGGRALAVRADALDAASVEDAVRQAVDAYGRIDILVNGVGGSTVVGRPAA
                     LLDELTLEEWQKLLHFNLTGTFLFCHAVIPVMKRQQSGKIVNLSSIAGRGLSQSSSSA
                     YATAKGGIIALTRKLAFELGPHGVTVNAIAPSLTLSERIRPHWERRTAEGQAAEIKRT
                     PLGRVATPEDQARVICFLASSDADFVTGVTIDVTGGQ"
     gene            2260..3315
                     /locus_tag="VFX87_13405"
     CDS             2260..3315
                     /locus_tag="VFX87_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX4995972.1"
                     /translation="MGEILGLGMTHYPPLTGLDENMAAILRHVQQDPGLPPRYRDPAS
                     WPAPMRAEYGTDGGTASAAGHREALVRHFRKARRILDEFRPDVVIVWGDDQHENFTED
                     VIPPFCVLAYDQVEARPWQRPGAAPNIWNEPADTTFRIAGHRRAGKYLATGLLEQGVD
                     MAYAYTPLHYPGLAHAFLNTIMFLDYDRVGFPYPVIAFQVNCYGRRVIAQRGGRASLA
                     NPLAEADLDPPSPSPKRCMEVGAATARVMRDSPWRTALIASSSWSHAFLTDKHHQLYP
                     DIESDRRLYEALRLGDYETWRKRPLEAIETSGQQEMLNWYMLVGAMEALGRKPDECDF
                     IETWSFNSNKCFATFMP"
     gene            complement(3433..4281)
                     /locus_tag="VFX87_13410"
     CDS             complement(3433..4281)
                     /locus_tag="VFX87_13410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4995973.1"
                     /translation="MNIGVSVPLPAYLVDVGAMARKAEELGFESFWCAEHPFIPVQTS
                     SRFPGSPDGVIPEEYSHFVDPFVALARASGTTSRIKLATGIVLVPERHPLVLAKEVST
                     LDLFSGGRFLFGIGAGWLREETQIMGGDFDHRWTQTRESVLAMKELWTKTDAEFHGKY
                     YDFPAVRSYPKPAQKPHPPVLLGGGAKNVLQRVASWGDGWLPNRVTPDQLRESRATLD
                     RLAKDAGRDPVTISISVHGQPADRDLVRRLLDAGATRVIIRPATSRTEQEMVRELTRI
                     AETVMR"
     gene            complement(4305..5084)
                     /locus_tag="VFX87_13415"
     CDS             complement(4305..5084)
                     /locus_tag="VFX87_13415"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4995974.1"
                     /translation="MILADRVAIVTGAGNGIGKATALALARAGARVAAVDVDGGAAKV
                     TAEAAAALGPRSLALDTDVGDLVAIDRMVQRVMETFGRIDVLVNNAGVTRRAYIMDLT
                     EQDWDRIMRVNAKGVFFCLQRVAREMIPRRSGTIINIASIAGKGYAGTSNAIYAASKG
                     SVISLTRTAALQLARHDINVNAICPGITVTALSEANVAVRARDEGLSLEDMTRRRNAV
                     IPLGRPNDPEDVAALAVFLASPGARNITGQSLNVDGGIIFD"
     gene            complement(5095..6711)
                     /locus_tag="VFX87_13420"
     CDS             complement(5095..6711)
                     /locus_tag="VFX87_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4995975.1"
                     /translation="MLTRREFLAATTAAAALAATPSGVAAQTPKRGGTLSVRAWDPPH
                     FDHSLVHAYKTHVVVSFTHSRLLKHRAGPGVRPGSLVLEGDLAESWTQPSDTSYVFKL
                     RRGVRFHAKPPVNGRELTAEDVKYTFERMLTETGSNASMYRSIAKIEAVDRYTVRFTL
                     SEPFAWFLDMVASPMAGGIVARECVEKFGDLKKPEAVIGTGPWMLESYKPNVSITLVR
                     NPAYFVAGLPYIDRVELMVDEDNASRMAAFLSGRYDLGWEFPGTINRTDWVQIKDRLS
                     RTRPSLRTAEFPSNVVNDLAMRTDQKPWSDVRVRQAVSMALDRREIIESTLEGVGVVN
                     GPIPAALTDWALPIDQLGEGARNYRHDPAAAKRLLAAAGYPNGFPASVCFNSYGSTVL
                     VDTMQLVLKHLKAVGIDARLDQKEYGAYQATCRLGKFDSLVFGPLTPFLDADNFLFGQ
                     YYTGEPRNRSHVKDPALDDLLVRQRRAMDVKARREIINDIQRHLAKQQYYVHAPAGTY
                     IAVWDGALKNYGPNLGYDYGSRVVAAWLDR"
     gene            complement(6779..8023)
                     /locus_tag="VFX87_13425"
     CDS             complement(6779..8023)
                     /locus_tag="VFX87_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995976.1"
                     /translation="MTPARLVACVCVAQVCAQIGAYTWPALLPGFLAEWQLTNREAGW
                     ITGLFYAAYTVSVPVLVTLTDRVDARTVYLSGVALTVASHLGFAVFVDGPWGAAAARA
                     LAGVGWAGTYMTGLKLLADRVEPVLMSRAVAGHAASIGIAGAISFVFAGTIGRWLGWR
                     GAFAAAGIAAIAGWLIVFVWAPARERRPATAASGALFDFRPVLRNRSAMAYAVAYCAH
                     TWEMNALRGWAVAFLAYVAAAAGDRSPWLGPTTVVMVMGLVGTWASVAGNEAAIRMGR
                     PRLVRLAMTACVVCAAGIGFLGARSYTVAAALVLLYGLLIWLDSSSLTAGAAGSADPE
                     RRGATLALHSMAGYAGGAVGPIVIGWILDLSGGMSPMGWGLAFLHVAVIALLGQVAFV
                     LLGPRDLVGDRRRPSGRSIDRA"
     gene            complement(8020..8436)
                     /locus_tag="VFX87_13430"
     CDS             complement(8020..8436)
                     /locus_tag="VFX87_13430"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX4995977.1"
                     /translation="MGRPHVLFLCTHNSARSQMAEGFLRTMAGDRFEAGSAGTEKTSV
                     NPLAIRVMAELGIDLGGHTSKLYADVAAEGWDFLITVCDDANERCPWVPGSVQRLHWS
                     FLDPSRATGTEEERLAVFRRVRDQIRARLIDWLRDR"
     gene            complement(8438..8932)
                     /locus_tag="VFX87_13435"
     CDS             complement(8438..8932)
                     /locus_tag="VFX87_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEX4995978.1"
                     /translation="MTTIRTRLATPADAAAITAIYNEGIADRVATFETEPRTVAQIAQ
                     QLADRGDRFPTVVAERDGQVIAYAGAGAYRSRPAYAGVAEHSVYVARAARGTGAGRAA
                     LDAMCRAYAERGFWKILSRIFPENTASLRLHERCGFRVVGVYQRHGKLDGQWRDCVIV
                     ERLL"
     gene            complement(8929..10203)
                     /locus_tag="VFX87_13440"
     CDS             complement(8929..10203)
                     /locus_tag="VFX87_13440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4995979.1"
                     /translation="MSLFAPGGPAWLLAICVSRVGAYMVYIAYAATLPVLQHEWQLSG
                     TAAGSIASAFQVAYAVSLMGCSALADRVGARRVFVVGTVVSAIVSIAFAMYARDYWSG
                     LGLYTLLALALGGTYTTGILLVAENVPVERRGRAMGAYLAGHSLGLALALTLAGLAIP
                     RGGYVLAFWLLALGPVVGAAFSWIAARTSPNIVTPRAGSQRFGGEVLRNRPAMLVIAG
                     YTFHSWELLGMWAWTPTFLAACFVAAGSELTRGAGLGAYMTALFHLTGMLAALLAGVF
                     ADRFGRTPVILVMATVSAICSLAFGWMLGASLVLIVAVGLLYGFAALGDSPIYSTAIT
                     EVVAPAYRGAALALRSLLGYGAGAAAPLVFGAILDWYGVRSAGAWGWAFLSLGIAGVG
                     AVVSVVLLHRTPAAAALHGPGMRRAPASAGAR"
BASE COUNT         1565 a         3743 c         3580 g         1409 t
ORIGIN      
        1 gtgcacgtgc gcccccagcg cgcgtcgggc tcgaactcga tgaccgtgat gccgggcacc
       61 gcctcaagca gccgccgtgc ggcccgccct tcgcgcgccc gcgcctcggt cgcgtagtga
      121 tagtgcaggg ccacggtggc ctcgacccgc tgggtgaacg tgaagagccc gcgctcgaga
      181 cagtcggcca ggaactcggc cgtgtggcgc ttcgggaacg gcagctcggc ctgcagcacc
      241 tcgtcgtaga actggatgca cgacgggcac cacatcacga cctcgcgcgg cgccatgctc
      301 tgcagcatct cgacggtgtg gttggcgtag cgctcgcccg aggcctcctg cccgcgccgg
      361 tggtgctgga tgccgcagca gtaggtcggc ccgcccgcgg ccacgtagtc gagcccgagc
      421 cggtcgaaga ccgcgcccac cgtctcgacc aggtgcgagg tccgcagcac gttgcagccg
      481 aggtagagga ccacgtcgtg gtgctcgccg tcccgcggcg gcttgagcag ccacgtgcgc
      541 tcctccttct tcgagaggag gtcgtgcgtc agcttgatct cgccgaagta cttgccgtaa
      601 tcgaagccca tggtgtcagg agtttaccac tacgggtcga cctcccgcag cctgcccctc
      661 tggcgggcca gccatgttac ggtggatggg accgtgaaca cgccgatccg cacgatcgcg
      721 atgctcgggc tgcttgtcct ggccaccgct tgctccaaag cggtcttggc cccggagccc
      781 ggcgcctccg gggtgccggc cgagggagtg gagccctccc gcaatgcgac cgccgcccac
      841 ctggcgacgc gctacctggg cgcgccctac gtgtgggccg gctcgagccc gtccggcttc
      901 gactgctccg gcctcgtcat gtatgccttc gggaaggtcg gcgtcgccat tcctcacaat
      961 gccgcgcagc agttccgtct cggcaccccg gtcccccgca atcgcctgat gccgggcgac
     1021 gttgtcttct tcaaccagct ccgccacaac ggcatctacc tgggagacgg gcgcttcgtc
     1081 cacgcgacga agccgggcgg ggtcaagatc gcacggctgg acgacgactg gttccggacc
     1141 cgctgggtgg gcgcgcgccg gctctagccg gggggcgtcc tctctaggcc tcgtactcct
     1201 ggaccgcttc gatctgcacg tcgccgtcgt cgtcccaccg cagcgtgatg aggttcggcc
     1261 ggcagcacac cccgcagtcc tcggtgaacg tctggtgccg cccgccgctg tcatcggcga
     1321 agacgtcgtt ctcctccccg cagaacgcgc agtcgtaggt cacgggcgcc attctaatcc
     1381 ggcttcgggg tatagtcccg gtcggaggag caagccatga cgcgattcca gggcaaggtg
     1441 gcgctgatct cggcggcggg ggccggcatc ggccgggcaa ccgcgatgat cgtcgcgcgc
     1501 gagggcggca ccgtggtggg cgtggatctg gagccggccc tgctcgatcg gctgaccgcc
     1561 gacgtcgtcg cggcgggggg gcgcgcgctc gccgtgcggg ccgacgcgct cgacgcggcg
     1621 tccgtggaag acgcggtccg gcaggccgtc gacgcctacg ggcgcatcga catcctggtc
     1681 aacggcgtcg gcggcagcac cgtggtcggc cgccccgccg ccctgctgga cgagctcacc
     1741 ctcgaggaat ggcagaagct gctccacttc aacctgaccg ggacgttcct cttctgccac
     1801 gcggtgatcc cggtgatgaa gcggcagcag agcggcaaga tcgtcaacct ctcctcgatc
     1861 gccgggcgcg ggctcagcca gtcgagctcg agcgcctacg ccaccgccaa gggcggcatc
     1921 atcgcgctca cccgcaagct ggccttcgag ctgggccccc acggcgtcac ggtcaacgcg
     1981 atcgcgccca gcctcacgct ctccgagcgc atccggccgc actgggagcg ccgcacggcg
     2041 gagggtcagg cggccgagat caagcgcacc ccgctcgggc gggtggccac gcccgaggac
     2101 caggcgcggg tcatctgctt cctggcctcg tccgacgccg acttcgtcac cggcgtcacc
     2161 atcgacgtga ccggcgggca gtagcgtaga ttgacaagcc ccggccccgg ggactagggt
     2221 tgcgcaagcg ctcgcatccc cgacgcggag gaacctctca tgggtgagat tctcgggttg
     2281 ggcatgacgc actacccgcc gctcaccggc ctggacgaga acatggccgc gatccttcgc
     2341 cacgtccagc aggaccccgg ccttccgccg cgctaccgcg atcccgcgag ctggccggcc
     2401 ccgatgcgcg cggagtacgg caccgacggc ggcaccgcga gcgcggccgg ccaccgcgag
     2461 gcgctggtcc ggcacttccg caaggcccgg cgtatcctcg acgagttccg cccggacgtg
     2521 gtgatcgtct ggggcgacga ccagcacgag aatttcaccg aggacgtgat cccgcccttc
     2581 tgcgtgctgg cctacgacca ggtcgaagcg cgcccgtggc agcgtcccgg cgccgccccg
     2641 aacatttgga acgagccggc cgacaccacc ttccgcatcg ccgggcaccg ccgggccggc
     2701 aagtacctgg cgaccgggct cctcgagcag ggcgtggaca tggcctacgc ctacacgccc
     2761 ctgcactacc cgggcctcgc gcatgcgttc ctgaacacga tcatgttcct cgactacgac
     2821 cgcgtcggct tcccgtaccc ggtcatcgcg ttccaggtca actgctacgg ccgccgggtg
     2881 atcgcccagc gcggcgggcg cgccagcctc gccaacccgc tcgccgaggc ggacctggat
     2941 ccgccgtccc cgtccccgaa gcgctgcatg gaggtgggcg ccgcgaccgc gcgggtcatg
     3001 cgcgacagcc cgtggcgcac cgccctcatc gcttcgtcga gctggtcgca cgccttcctc
     3061 accgacaagc accaccagct ctatcccgac atcgagtcgg accggcggct ctacgaggcg
     3121 ctgcgtctgg gcgactatga aacatggcgc aagaggccgc tcgaggccat cgagacgtcg
     3181 ggccagcagg agatgctcaa ctggtacatg ctggtcggcg ccatggaggc cctggggcgc
     3241 aagccggacg agtgcgactt catcgagacg tggtccttca actcgaacaa gtgcttcgcc
     3301 acgttcatgc cctgaggata tccgccccga acgcctgatc cgcatcagca gccggttgac
     3361 agtttccgac aggtccgacg cgtacggtcc gcgctcaact ggttgattcg attaccttgg
     3421 cctctggcgc acctaccgca tcaccgtctc cgcgatccgg gtcagctcgc gcaccatctc
     3481 ctgctccgtc ctggacgtgg ccggtcggat gatgacgcgg gtggcgcccg cgtcgaggag
     3541 gcggcgcacc agatcccggt cggcgggctg gccgtgcacc gagatggaga tcgtgacggg
     3601 atcgcggccg gcgtccttgg cgagccggtc gagggtggcg cggctctcgc ggagctgatc
     3661 gggcgtcacg cggttgggca gccagccgtc gccccaggac gccacgcgct gcagcacgtt
     3721 cttcgcgccg ccacccagca gcaccggcgg gtgcggcttc tgggcgggct tgggatagga
     3781 acgcaccgcc gggaagtcgt agtacttgcc atggaactcg gcgtccgtct tcgtccacag
     3841 ctccttcatg gccaggaccg actcccgcgt ctgggtccac cggtggtcga agtcgccgcc
     3901 catgatctgg gtttcctcgc ggagccagcc ggccccgatg ccgaagagga agcgcccccc
     3961 gctgaagaga tcgagcgtgg agacctcctt ggccagcacc agcgggtgcc gctccggcac
     4021 cagcacgatc ccggtggcga gcttgatgcg gctggtcgtc cccgaggcgc gggccagggc
     4081 cacgaaggga tccacgaagt gcgaatactc ctccgggatc acgccgtccg gcgagccggg
     4141 gaaccggctg ctggtctgca ccgggatgaa cgggtgctcg gcgcaccaga aagactcgaa
     4201 gcccagctcc tcggccttgc gggccatggc gccgacgtcc acgagatagg caggcagggg
     4261 caccgagacg ccgatgttca tggtgaggtc tccttgatcc tgaatcagtc gaagatgatg
     4321 ccgccgtcca cgttgagcga ttggccggtg atgttccggg cgcccgggga agccaggaac
     4381 accgcgagcg cggccacgtc ctccggatcg ttcggccgac cgagcgggat gaccgcgttg
     4441 cgccggcgcg tcatgtcctc cagcgacagc ccctcgtcgc gggcgcggac tgccacgttg
     4501 gcctcggaca gcgcggtgac cgtgatgccg gggcagatcg cgttcacgtt gatgtcgtgg
     4561 cgggcgagct ggagcgcggc ggtgcgggtc agcgagatca cgctgccctt gctggccgca
     4621 tagatcgcgt tcgaggtgcc cgcgtagccc ttgcccgcga tcgatgcgat gttgatgatc
     4681 gtgccgctcc gccgggggat catctcgcgc gccacccgct gcaggcagaa gaagacgccc
     4741 ttggcgttga cgcgcatgat gcggtcccag tcctgctcgg tgagatccat gatgtaggcg
     4801 cgccgcgtga ccccagcatt gttcaccaag acgtcgatcc gcccgaacgt ctccatcacg
     4861 cgctggacca tccggtcgat ggcaaccaga tcgcccacgt cggtgtccag cgccaggctc
     4921 cgaggcccca gcgccgcggc ggcctccgcg gtcaccttgg cggcgccccc gtccacgtcc
     4981 accgcggcga cccgcgcgcc cgcccgcgcg agggccagcg cggtggcctt cccgatgccg
     5041 ttgccggcgc cggtgacgat cgcaacgcga tcggcgagga tcatgcgcgc gcgatcagcg
     5101 gtcgagccag gcggcgacga cccggctgcc atagtcgtag cccaggttcg gcccgtagtt
     5161 cttgagcgcg ccatcccaca ccgcgatgta ggtcccggcg ggcgcgtgca cgtagtactg
     5221 ctgcttggcg aggtgccgct ggatgtcgtt gatgatctcg cggcgggcct tcacgtccat
     5281 cgcgcgccgc tgccggacga gcaggtcgtc gagggcggga tccttgacgt ggctccggtt
     5341 gcgcggctcg ccggtatagt actgcccgaa caggaagttg tccgcgtcga ggaacggggt
     5401 caggggcccg aacaccagcg agtcgaactt cccgagccgg cacgtcgcct ggtaggcgcc
     5461 gtactccttc tgatccagcc gcgcgtcgat gccgaccgcc ttcagatgct tgagcacgag
     5521 ctgcatggtg tccaccagca cggtggagcc gtacgagttg aagcacacgc tggccgggaa
     5581 cccgttcggg tagccggccg ccgcgagcag ccgcttggcc gccgcgggat cgtgccgata
     5641 gttgcgcgcg ccctcgccga gctggtcgat cggcagcgcc cagtcggtca gcgcggccgg
     5701 gatcgggccg ttgacgacgc ccaccccctc gagcgtggac tcgatgatct cgcgccggtc
     5761 cagcgccatc gagaccgcct ggcgcacccg cacgtcgctc cagggcttct ggtcggtgcg
     5821 catcgcgaga tcgttcacta cgttcgacgg gaactcggcg gtccggagcg agggccgcgt
     5881 ccggctgagg cggtccttga tctgcaccca gtccgtccgg ttgatcgtcc ccggaaactc
     5941 ccagccgagg tcgtagcggc ccgagaggaa cgcggccatg cgcgaggcat tgtcctcgtc
     6001 caccatcagc tcgactcgat cgatgtacgg caggcccgcc acgaagtaag ccgggttccg
     6061 caccagggtg atcgacacgt tgggcttgta gctctcgagc atccaggggc cggtgccgat
     6121 caccgcctcc ggcttcttga ggtcgccgaa cttctccacg cactcccggg ccacgatccc
     6181 gcccgccatc gggctcgcca ccatgtcgag gaaccaggcg aagggctccg agagcgtgaa
     6241 ccggaccgtg tagcgatcca ccgcctcgat cttggcgatc gaccggtaca tcgaggcatt
     6301 gctgcccgtc tcggtgagca tgcgctcgaa cgtgtacttc acgtcctccg cggtgagctc
     6361 ccggccgttc accggcggct tggcgtggaa gcgcacgccg cggcggagct tgaagacgta
     6421 gctggtgtcg ctcggctggg tccaggactc ggccagatcg ccttcgagga ccagcgagcc
     6481 gggccgcacg ccggggcccg cgcgatgctt caggagccgg ctgtgggtga aggagaccac
     6541 cacgtgcgtc ttgtacgcgt gcaccaggct gtggtcgaag tgcggaggat cccaggcccg
     6601 gacggacagg gtgccgccgc gcttgggggt ctgggcggct acgcccgacg gcgtggcggc
     6661 cagagctgca gcggcagtgg tggcggcgag aaactcacgg cgcgtgagca tagggggcgg
     6721 cctcctgggc gcgtcgttgc gcgcggctcc cgatgctcca ccggaccggg cggcttcgtc
     6781 aagcgcgatc gatcgagcgg cccgacgggc ggcgccgatc cccgaccaga tcgcgcggcc
     6841 ccaggagcac gaacgccacc tggcccagca gcgcgatgac cgccacgtgc aggaaggcaa
     6901 ggccccagcc catgggcgac atgccgccgc tcaggtcgag gatccacccg atcacgatcg
     6961 gcccgacggc cccgcccgcg tagcccgcca tcgagtgcag tgccagcgtg gcgccgcggc
     7021 gctcggggtc cgcgctgccg gccgcgcccg cggtgagcga ggaggagtcg agccagatca
     7081 gcagcccgta gaggagcacg agggccgccg ccaccgtgta cgagcgggcg ccgaggaagc
     7141 cgatgccggc cgcgcacacc acgcacgcgg tcatggccag gcgcacgagc cggggccggc
     7201 ccatgcggat ggcggcttcg ttgccggcca cgctcgccca tgtgccgacg agccccatga
     7261 ccatcaccac ggtggtggga ccgagccagg ggctgcggtc gccggcggcg gccgccacgt
     7321 aggccaggaa cgcgaccgcc cacccgcgca gggcgttcat ctcccacgta tgcgcgcagt
     7381 aggcgacggc ataggccatg gccgagcggt tgcggagcac cggccggaaa tcgaagagcg
     7441 ccccggaggc cgcggtggcc ggccgccgct cccgcgccgg cgcccagacg aacacgatca
     7501 gccagccagc gatcgcggcg atcccggcgg ccgcgaaggc gccgcgccag ccgagccatc
     7561 ggccgatcgt gcccgcgaac acgaacgaga tcgcgccagc gatgccgatg ctggcggcgt
     7621 gtccggccac cgcgcgcgac atgagcaccg gctccacccg gtcggccagg agcttgagcc
     7681 cggtcatgta ggtgccggcc cagcccaccc ccgccagcgc gcgtgccgcg gcggcgcccc
     7741 acggcccgtc cacgaagacg gcgaagccca ggtggctcgc caccgtgagg gccacgccgc
     7801 tcaggtagac ggtccgcgcg tccacgcggt cggtgagcgt gacgaggacg ggcaccgaca
     7861 ccgtgtaggc cgcgtagaag agcccggtga tccagcccgc ctcccgattg gtgagctgcc
     7921 actcggcgag gaagcccggc agcagcgccg gccacgtgta ggccccgatc tgggcgcaca
     7981 cctgcgccac gcagacgcac gcgacgagcc gcgcgggcgt catcggtcgc ggagccagtc
     8041 gatcaggcgc gcccggatct ggtcacgaac ccggcggaag acggcgagcc gctcctcctc
     8101 ggtgcccgtc gcgcgcgagg gatcgaggaa tgaccagtgc agccgctgca ccgagccggg
     8161 gacccagggg caccgctcgt tcgcgtcatc gcacaccgtg atgaggaagt cccagccctc
     8221 cgcggccacg tccgcgtaga gcttcgaggt gtgcccgccg aggtcgatgc cgagctcggc
     8281 catgacccgg atcgccagcg gattgaccga ggtcttctcg gtcccggcgc ttccggcctc
     8341 gaagcggtcg ccggccatcg tgcggaggaa gccctcggcc atctggctcc gggccgagtt
     8401 gtgcgtgcag aggaacagga cgtgcggccg ccccacgtca gagcagccgc tcgacgatca
     8461 cgcagtcgcg ccactggccg tcgagcttgc cgtgtcgctg gtagacaccg accacccgga
     8521 agccgcaccg ctcgtgcagc cgcaggctcg cggtgttctc gggaaagatc cgagagagga
     8581 tcttccagaa gccgcgctcg gcgtacgccc ggcacatcgc gtcgagcgcc gcccgtccgg
     8641 cgccggtgcc ccgcgcggcg cgcgccacgt acaccgagtg ctcggcgacg cccgcgtagg
     8701 caggccggct ccggtacgcg cccgcgcccg cgtacgcgat cacctgcccg tcccgctctg
     8761 ccaccaccgt cgggaagcgg tcaccccggt ccgcgagctg ctgcgcgatc tgggccaccg
     8821 tccgcggctc ggtctcgaag gtcgcgaccc ggtcggcgat cccctcgttg tagatggcgg
     8881 tgatggcggc cgcgtcggcc ggcgtcgcca gtcgggtgcg gatcgtggtc atcgggctcc
     8941 cgccgaggcg ggggcccggc gcatgccggg cccgtgaagg gcggccgccg ccggcgtccg
     9001 gtgcagcagc accaccgaca ccacggcgcc cacgccggcg atgccgaggc tcaggaacgc
     9061 ccagccccac gcgccggcgc tgcgcacccc gtaccagtcg aggatcgcgc cgaacacgag
     9121 cggcgcggcg gcgcccgcgc cgtagcccag gagggagcgc agcgccagcg cggcgccgcg
     9181 ataggcggga gccaccacct cggtgatcgc ggtcgagtag atcggcgagt ccccgagcgc
     9241 ggcgaacccg tagaggagcc ccaccgcgac gatcaggacg agcgaggccc cgagcatcca
     9301 gccgaacgcc agcgagcaga tcgcgctgac cgtcgccatc acgaggatca ccggcgtccg
     9361 accgaagcga tccgcgaaca cgccggcgag cagcgcggcc agcatcccgg tcaggtggaa
     9421 gagcgccgtc atgtaggcgc cgaggcccgc gccgcgcgtg agctccgagc cggccgccac
     9481 gaagcaggcg gccaggaacg tgggcgtcca cgcccacatg ccgagcagct cccaggagtg
     9541 gaacgtgtac ccggcaatga ccagcatggc gggccggttc ctgagcacct ccccgccgaa
     9601 gcgctggctc cccgcgcggg gggtcacgat gttggggctc gtccgcgccg cgatccagga
     9661 gaaggcggcc cccaccaccg gcccgagggc caggagccag aaggccagca cgtacccgcc
     9721 gcgcgggatc gccaggccgg ccagcgtgag ggccagcgcg aggccgagcg agtggccggc
     9781 caggtacgcc cccatcgcgc gcccgcgccg ctcgaccggc acgttctcgg ccacgagcag
     9841 gatgccggtc gtgtaggtac cgcccagggc gagcgcgagc agcgtgtaga gacccagccc
     9901 cgaccagtag tcgcgggcgt acatcgcgaa cgcgatcgac acgatggccg acaccacggt
     9961 cccgaccacg aagacacgcc gtgcgccaac tcgatccgcc agcgcggagc accccatcag
    10021 cgacaccgcg tacgcgacct ggaacgcgga cgcgatgctg cctgccgcgg tgccggagag
    10081 ctgccactca tgctgcagga cgggcagcgt cgccgcgtag gcgatgtaca ccatgtaggc
    10141 gcccacccgc gacacgcaga tggcgagcag ccacgcgggg ccgcccggag cgaacaggct
    10201 catctgcggc tgggtgggag gcggtgaaac agcccggacg cggtccctcc cgcctccgcc
    10261 gccacccgcc gaccgaggcg tgcccgatcg acaaccg
//
LOCUS       DASUFA010000366         7649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1006648, whole genome shotgun sequence.
ACCESSION   DASUFA010000366 DASUFA010000000
VERSION     DASUFA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006648"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFX87_13445"
     CDS             <1..819
                     /locus_tag="VFX87_13445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX4995980.1"
                     /translation="WPVVIAPMGGLVLFHPEGDCEMARGATKGDTLQFLSGATGWSVE
                     AVAAAAPGPKMFQLYHMGDRAWVADLLARVEASGYLSVCLTVDVQVYSRRERDIVARF
                     TPREAMLKAPNPRPPDPDYPARLTWTDVDWLRKTTKLPIGLKGIMTARDARRAVEAGV
                     ELIWVSNHGGRQLDATQASIDALPAVAEAVGGAASIIVDGGFARGTDVIKGIAWGADA
                     VAIGRTALWGLAADGAAGVACALDILRRELRTSMALAGQTSVRGLTPDVVFRVD"
     gene            complement(828..1001)
                     /locus_tag="VFX87_13450"
     CDS             complement(828..1001)
                     /locus_tag="VFX87_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995981.1"
                     /translation="MRELACLLLLALAGCGAPIERPYTEEELEVQCVMRGGWWHRDDL
                     MGGFCEFQAPGFL"
     gene            complement(1002..1328)
                     /locus_tag="VFX87_13455"
     CDS             complement(1002..1328)
                     /locus_tag="VFX87_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX4995982.1"
                     /translation="MLYELRTYHAMPGRLPDLNRRFAEITMDFFKKHNIQVVGFWTNE
                     LGGSSDQLIYMLAWDSLADREKKWSGFFADKDRLAKFAETEKNGPLLRRLTAHILRPT
                     AYSPMQ"
     gene            1405..2154
                     /locus_tag="VFX87_13460"
     CDS             1405..2154
                     /locus_tag="VFX87_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX4995983.1"
                     /translation="MRSRLSRAASITARVGLVLTLLGALPLLLAGAGSRWDWWDFRTG
                     FFIFRWTLYGGLGAVVVALAGLVLALLARANNTALLSIGTLVLLAAVAVVPVSHLRGA
                     GRVPPIHDITTDLDDPPRFVAVLPRRQGAPNTAEHGGAALAAQQRAGYPDLGPARFPA
                     PPDRVFARAVDVARSLGWEIVAAAPAEGRLEATDRTRWFGFRDDVVVRVRPDGTGSRV
                     DVRSVSRVGRSDLGTNARRIRAFLESLRAGL"
     gene            complement(2204..3370)
                     /locus_tag="VFX87_13465"
     CDS             complement(2204..3370)
                     /locus_tag="VFX87_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX4995984.1"
                     /translation="MKITTVRATWLRAPIPAEHAHVSDFGRNDTFNMCLVEIETDTGL
                     VGLGEAKAAVGNLGNYAAMVTLIREEMGPAIVGRDPRDITGIWELLYNGSRARYAARE
                     GRTFPTIGRRGLALSAISGIDIACWDLLGRSVGQPVWRLLGGQYRDRIPAYASGGWAP
                     VGAIGKQMTQYVERGHRAVKLRVGLQDKSVDDSAARVREVRETLGPDVGIMVDAHGTW
                     SVREAMRFTRKVEDCDLGWFEEPVSSENLAGQAEVRASTDIPIAAGESEQTRFAFRDM
                     IAARAVDVCQPDVAIAGGITETQRIAALAAAHDLTVAPHLWGSAILFAAGLHLAVSTP
                     CVNLLEFSRGHNPLLNDLVEERVELEDGHVLAPAGPGLGLTLRRDFVARVAVSP"
     gene            complement(3402..4451)
                     /locus_tag="VFX87_13470"
     CDS             complement(3402..4451)
                     /locus_tag="VFX87_13470"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX4995985.1"
                     /translation="MLTPVEHAPLAPHCTLRIGGPARYWVEATDEATVAEAVQWAAAR
                     KLPVRVLGGGSNLVIADEGVDGLVLKIGLRGVSAREIPGAAEVTVAAGEPWDDFVRHT
                     VERGWAGLECLSGIPGLVGATPIQNVGAYGQEVSDTLAAVRALDRESGRVVTLAPAEC
                     GFGYRDSLFKSRAPGRYVVLAVTYRLVPGGAPYLGYADIAREIGTASPSLATVRETVI
                     RVRRSKSMVLDPGDPNARSCGSFFLNPVVDAAALAAIEARAAGLTMPRWPQPDGRVKL
                     SAAWLIERAGFTRGQADGPVGLSTRHTLAIVCHDGARARDVIGFARRVRAGVEARFGV
                     RLHPEPIFWGTLSLD"
     gene            complement(4537..5658)
                     /locus_tag="VFX87_13475"
     CDS             complement(4537..5658)
                     /locus_tag="VFX87_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995986.1"
                     /translation="MLGQRSPISRGVLMAVACLILLAIISAASVTAQRLADGQAPRFT
                     VDPFWPKPLPNRWIFGQVAGVATDNLDHVWIVQRPRSLTDDEKGATLSPPRNECCVPA
                     PAVMEFDSEGNLLQAWGGPGPGYDWPANEHGIFVDQAGNVWLAGNGEKDHQILKFSRD
                     GRFLLQIGKPASGGGDTDTRNLNRPADVRVDASANELYVADGYANHRVIVFDATTGAY
                     KRHWGANGRPPGDASVKQFKNPVHCVRVAADGLVYVCDRMNNRIQVFKKDGAFVGEFT
                     LAPTTGGNGSVWDLDFSRDPAQSYLYVADGENNHVWMLSRSSGKVLSRFGRSGRYAGQ
                     LHWVHNMAVDSRGNIYTAEVDNAKRVQKFVYQGLFPVTD"
     gene            complement(5750..6271)
                     /locus_tag="VFX87_13480"
     CDS             complement(5750..6271)
                     /locus_tag="VFX87_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX4995987.1"
                     /translation="MGDLALLTWTFLWLSLLCVGGGLGVIPEMERQSVTIHGWLTARE
                     FVDGYTLTQLTPGPNMLIAVFVGYRAHGAPGAVLAGLAMFLPTSVLAMVISHHWQQWR
                     TRRWAATAERALIPVGVGLMAGGVYTLARTAIHDTVTLALAVLTALAVATDRVPAVLA
                     VLAAGTLGWLLSR"
     gene            complement(6271..6855)
                     /locus_tag="VFX87_13485"
     CDS             complement(6271..6855)
                     /locus_tag="VFX87_13485"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX4995988.1"
                     /translation="MRRVSLLRLIAVFTWTGLSSLGGGRSAYFHEALVRRRGWIRNDE
                     FVQDFTLSQVLPGPNFSNLAVALGYRLAGMAGGVWGLLAIVLPGAFILVGAAILYLHG
                     AFGGSATPLIRSLSAAVVGLVCVTTLRIVRGSLRGGRGVVVAAVTFLAVGPLRLSTVG
                     VVLVMATVSLALHWAAPARSAPPAETTTSPEEIC"
     gene            complement(6975..7373)
                     /locus_tag="VFX87_13490"
     CDS             complement(6975..7373)
                     /locus_tag="VFX87_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995989.1"
                     /translation="MKKLIALVIAAAVLMPGLAFAGASTDAALGLGAFAVFNQILGGV
                     GIFHRGPVVAAAPPVVYAPPPPVYYAPPAPAYVPAPRVIVKPYPVYVPAPRHVVVYPQ
                     PAFCPPGHARKGWCYGGAGYRPVPQPGWRH"
     gene            7500..>7649
                     /locus_tag="VFX87_13495"
     CDS             7500..>7649
                     /locus_tag="VFX87_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014875141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4995990.1"
                     /translation="MSEHASVCPLDCPDTCSLTVTVEHAKIVKIRGSRANPYTAGVIC
                     TKVAGS"
BASE COUNT         1096 a         2740 c         2786 g         1027 t
ORIGIN      
        1 tggccggtgg tgatcgcgcc catgggcggg ctcgtcctct tccatcccga gggcgattgc
       61 gagatggcgc gcggcgccac caagggcgac acgctccagt tcctgagcgg cgccaccggc
      121 tggtcggtgg aggcggtggc ggcggccgcc cccggcccca agatgttcca gctctaccac
      181 atgggcgacc gggcctgggt ggccgacctg ctggcgcggg tggaagcctc cggctacctc
      241 tcggtgtgtc tcacggtgga tgtgcaggtc tacagccggc gcgagcgcga catcgtcgcg
      301 cgcttcaccc cccgcgaggc catgctcaag gcgccgaacc cgcgcccgcc cgacccggac
      361 tacccggcgc gcctcacctg gaccgacgtg gactggctgc gcaagaccac gaagctcccc
      421 atcgggctca agggcatcat gaccgcgcgc gacgcccggc gcgcggtcga agccggcgtg
      481 gaactgatct gggtgtccaa tcacggcggc cggcagctcg acgccaccca ggccagcatc
      541 gacgcgctgc ccgcggtggc ggaagcggtc ggcggcgcgg ccagcatcat cgtggacggc
      601 ggcttcgccc gcggcaccga cgtgatcaag ggcatcgcgt ggggcgccga cgcggtggcg
      661 atcgggcgca ccgcgctctg gggactcgcc gcggacgggg cggccggggt ggcgtgcgcg
      721 ctcgacatcc tgcggcgcga gctgcgcacc tccatggccc tcgccgggca gaccagcgtg
      781 cgcggactca ccccggacgt ggtcttccgc gtggactgag ccgccgccta gaggaagccc
      841 ggcgcctgga actcgcagaa accgcccatc aggtcatcgc ggtgccacca gcccccgcgc
      901 atcacgcact gcacctccag ctcctcctcg gtgtatggcc gctcgatcgg cgcgccacag
      961 cccgcgagcg cgaggaggag caggcacgcg agttcccgca tctactgcat cggcgagtag
     1021 gcggtcggcc gcaggatgtg ggcggtgagc cgacgcagga gcggcccgtt cttctcggtc
     1081 tcggcgaact tggccagccg gtccttgtcg gcgaagaacc cgctccactt cttctcgcgg
     1141 tcggcgaggc tgtcccaggc gagcatgtag atgagctggt ccgaggagcc gcccagctcg
     1201 ttggtccaga agccgaccac ctggatattg tgcttcttga agaagtccat ggtgatctcg
     1261 gcgaagcggc ggttgagatc ggggagccgg ccgggcatcg catgatacgt ccgaagctcg
     1321 tacagcatga atggggcctc ctgggcaaca cgatgggtgc ggtctgccgc gactctaggc
     1381 acgcctcccg cccgtgtcaa gcgcgtgaga tctagactct cgcgcgcggc ctcgatcacg
     1441 gcacgcgtcg gcctcgtcct cactctgctc ggggcgctcc cgctcctgct cgcgggcgcg
     1501 ggcagccgct gggactggtg ggacttccgc accggcttct tcatcttccg ctggacgctc
     1561 tacggcgggc tcggcgcggt ggtcgtcgcg ctggccggcc tcgtgctggc gctgctcgcg
     1621 cgggcgaaca acaccgcgct cctgtcgatc gggaccctcg tgctgctggc cgcggtcgcg
     1681 gtggtgccgg tctcgcacct gcgcggcgcg ggccgcgtgc ccccgatcca cgacatcacc
     1741 accgatctcg acgacccccc gcggttcgtg gcggtgctcc cgcgccggca gggcgcgccc
     1801 aataccgcgg agcacggcgg cgccgcgctc gcggcccagc agcgcgcggg ctatcccgac
     1861 ctcgggcccg cgcgcttccc cgccccgccg gaccgcgtgt tcgcccgcgc ggtggacgtc
     1921 gcgcgcagtc tcggctggga gatcgtggcc gccgcgcccg cggaggggcg gctcgaggcc
     1981 accgaccgga cccgctggtt cggcttccgc gacgacgtgg tggtccgcgt gagacccgac
     2041 gggacgggca gccgcgtgga cgttcgctcg gtctcccggg tggggcgcag cgacctcggc
     2101 accaacgcgc gccggatccg ggcgttcctg gaatcgctgc gggcgggact ctaggcgcta
     2161 ccgcgcgacc cgcgacacct cgtcgagcgc gacctccggc aggtcacgga ctcaccgcga
     2221 cccgggcgac gaaatcgcgg cgcagcgtga ggccgagccc ggggccggcg ggcgccagca
     2281 cgtggccgtc ctcgagctcc acgcgctcct ccaccaggtc gttcagcagc ggattgtggc
     2341 cgcgggagaa ctcgagcagg ttcacgcacg gggtggacac cgccaggtgc aggcccgccg
     2401 cgaagaggat cgccgagccc cagagatgcg gcgccaccgt gaggtcgtgg gcggccgcga
     2461 gcgccgcgat gcgctgggtc tcggtgatgc cgcccgcgat ggccacgtcg ggctggcaga
     2521 catccaccgc gcgcgccgcg atcatgtcgc ggaacgcgaa gcgcgtctgc tcgctctccc
     2581 ccgccgcgat cgggatgtcg gtggaggcgc gcacctccgc ctggcccgcg aggttctccg
     2641 aggacaccgg ctcctcgaac cagccgagat cgcaatcctc gactttgcgc gtgaagcgca
     2701 tggcttcgcg cacgctccag gtgccgtgcg catccaccat gatgcccacg tcgggcccga
     2761 gcgtctcgcg cacctcgcgc acgcgcgccg ccgaatcgtc cacgctcttg tcctggagcc
     2821 ccacccgcag cttcaccgcg cggtgcccgc gctccacgta ctgggtcatc tgcttgccga
     2881 tggcgcccac cggcgcccag ccgccgctcg cgtaggccgg gatccggtcg cggtactgcc
     2941 cgcccaggag ccgccacacc ggctggccga cggagcgccc gagcagatcc cagcacgcga
     3001 tgtcgatgcc gctgatcgcg ctgagcgcga gaccgcgccg gccgatggtg gggaaggtgc
     3061 gcccttcgcg ggcggcgtag cgggcgcggc tgccgttgta gagcagctcc cagatcccgg
     3121 tgatgtcgcg cgggtcgcgg cccacgatgg cggggcccat ctcctcgcgg atgagcgtga
     3181 ccatcgccgc gtagttcccg agattgccca ccgcggcctt ggcctcgccg agccccacca
     3241 gcccggtgtc ggtctcgatc tcgaccaggc acatgttgaa ggtgtcgttg cggccgaagt
     3301 cgctgacgtg ggcgtgctcg gctggaatgg gggcgcggag ccaggtggcg cggacggtgg
     3361 tgatcttcat ggacgctcct tcggatggag gtgacggtgc ttcagtcgag gctcagggta
     3421 ccccagaaga tcggctccgg gtgcagccgc acgccgaagc gcgcttccac ccccgcccgc
     3481 acgcgacggg cgaagccgat gacgtcgcgg gcgcgcgcgc cgtcgtggca aacgatggcc
     3541 agggtgtgac gcgtggagag gccaacgggc ccgtccgcct gtccgcgcgt gaagccggcg
     3601 cgctcgatga gccacgccgc cgagagcttc acgcggccgt cgggctgggg ccagcgcggc
     3661 atcgtgaggc cggccgcgcg ggcctcgatg gcggcgagcg cggccgcgtc cacgacggga
     3721 ttcaggaaga aggagccgca gctccgcgcg ttcggatccc cggggtcgag caccatggac
     3781 ttggagcggc ggacgcggat cacggtctcg cgcacggtgg ccagcgaggg cgacgcggtc
     3841 ccgatctccc gcgcgatatc ggcatagccg aggtagggtg ccccgccggg gacgagccga
     3901 taggtgacgg ccaggaccac gtagcggccg ggcgctcggc tcttgaagag gctgtcgcgg
     3961 tagccgaagc cgcactcggc gggcgcgagc gtgaccacgc gaccgctctc gcggtcgagc
     4021 gcccgcaccg cggcgagcgt gtcgctcacc tcctggccgt aggcgcccac gttctggatc
     4081 ggggtggcgc ccaccaggcc ggggatgccg ctcaggcact cgagccccgc ccagccgcgc
     4141 tccaccgtgt ggcgcacgaa gtcgtcccac ggctcgcccg cggcgaccgt cacctccgcc
     4201 gcgccgggga tctcgcgcgc gctcacgccg cgcaggccga tcttgaggac gaggccgtcc
     4261 acgccctcgt cggcgatgac caggttggag ccaccgccga ggacgcgcac cggcagcttg
     4321 cgagcggccg cccactggac agcttcggcg acggtcgcct cgtccgtggc ctcgacccaa
     4381 tagcgggcgg ggccgccgat gcggagagtg cagtgggggg cgagcggggc gtgctcgacg
     4441 ggggtgagca tgggcgcgtc catcttaccg cgcccgcggt ttccggcccc cctcaccctg
     4501 ccctctcccc caagggggag agggagacag ggtggatcag tctgtgaccg ggaagaggcc
     4561 ctggtagacg aacttctgga cgcgcttggc gttgtccacc tcggcggtgt agatgttgcc
     4621 gcgcgagtcc accgccatgt tgtggaccca gtggagctgg cccgcgtagc ggccgctgcg
     4681 cccgaagcgc gagaggacct tgcccgagga ccgcgagagc atccacacgt ggttgttctc
     4741 gccgtcggcc acgtagaggt agctctgcgc cggatcgcgg gagaaatcga ggtcccacac
     4801 cgagccgttg ccgccggtgg tcggggccag cgtgaactcc cccacgaagg cgccgtcctt
     4861 cttgaagacc tggatccgat tgttcatccg gtcgcacacg tagacgagcc cgtcggcggc
     4921 gacgcgcacg cagtgcaccg ggttcttgaa ctgcttgacg ctcgcgtcgc ccggcggccg
     4981 cccgttggcg ccccagtggc gcttgtaggc gccggtggtg gcgtcgaaga cgatgacgcg
     5041 gtggttggcg tagccgtcgg ccacgtacag ctcgttcgcg gaggcgtcca cccgcacgtc
     5101 ggcgggccgg ttcaggttcc gcgtgtccgt gtcgccaccg cccgaggcgg gcttgccgat
     5161 ctgcagcagg aagcggccgt cgcgcgagaa cttgaggatc tggtggtcct tctcgccgtt
     5221 gccggccagc cacacgttgc cggcctgatc cacgaagatg ccgtgctcgt tggccggcca
     5281 gtcgtagccg ggccccggcc cgccccaggc ctggagcagg ttgccttcgc tgtcgaactc
     5341 catcaccgcg ggcgcgggga cgcagcactc gttgcgcggc gggctcagcg tggcgccctt
     5401 ctcgtcatcg gtgagcgacc gcgggcgctg gacgatccac acgtgatcga ggttgtcggt
     5461 cgccacgccc gccacctggc cgaagatcca gcgattgggc aggggcttgg gccagaaggg
     5521 atccaccgtg aacctcggcg cctgcccgtc cgcgagccgc tgcgcggtga ccgaggcggc
     5581 cgagatgatc gcgaggagga tgagacaggc gacggccatc agcacaccgc gcgagatcgg
     5641 ggatcgctgg ccaagcatgg cattcctcct cgagggttgc gggtacttca acggtggtag
     5701 gccggggcga cgatatgccg gtcgcgccgt cggtgtcaag cgcggggcgt caccgcgaga
     5761 ggagccagcc gagagttccc gcggccagca ccgcgagcac cgcgggcacg cggtcggtgg
     5821 ccaccgcgag cgcggtgagc accgcgagcg ccagggtcac cgtgtcgtgg atcgcggtgc
     5881 gcgccagcgt gtacacgccg cccgccatga gcccgacccc gacggggatg agcgcccgct
     5941 cggcggtggc ggcccagcgc cgggtgcgcc attgctgcca gtggtgcgag atcaccatgg
     6001 ccagcaccga ggtgggcagg aacatcgcga ggcccgcgag gacggcgccg ggcgcgccgt
     6061 gggcgcggta gcccacgaag accgcgatca gcatgttcgg gcccggggtg agctgggtga
     6121 gcgtgtagcc gtccacgaac tcgcgcgcgg tgagccagcc gtggatggtc accgactgcc
     6181 gctccatctc cgggatcacc ccgagcccgc cgcccacgca gagcaggctc agccagagaa
     6241 acgtccaggt gaggagcgcc aggtccccca tcagcagatc tcctcgggtg aggtcgtcgt
     6301 ctccgccggc ggggccgagc gcgccggcgc ggcccagtgc agcgcgaggc tcaccgtcgc
     6361 catcacgagc acgaccccca cggtggacag gcggagcggg cccaccgcga ggaaggtgac
     6421 cgcggccacc acgaccccgc ggccgccgcg gagcgagccc cgcacgatgc gcagcgtggt
     6481 cacgcacacg aggcccacca ccgccgcgct gaggctgcgg atcagggggg tggcggagcc
     6541 gccgaacgcg ccgtgcaggt agaggatggc cgcgcccacc aggatgaagg cgcccggcaa
     6601 cacgatggcg agcaggcccc acacgccgcc cgccatgccg gcgagccggt agccgagcgc
     6661 caccgcgaga ttgctgaagt tgggtcccgg gagcacctgg ctcagcgtga agtcctggac
     6721 gaactcgtcg ttgcggatcc agccgcgtcg ccgcaccagc gcctcgtgga agtaggcgct
     6781 gcgaccgccg ccgaggctcg agagcccggt ccaggtgaag accgcgatca gccggaggag
     6841 ggagacgcgc ctcacgagcc cagcctcact gagagggctc gagcgagcgc gggtccgaca
     6901 tgtcccctgg agtgtagcac cctctccccc cggggccggg gggagagggc cgagatggga
     6961 agcgagttac gagatcagtg gcgccagccg ggctgaggga ccggccgata ccccgcgccg
     7021 ccgtagcacc agcccttcct cgcgtggccg ggcgggcaga acgcgggctg cgggtacacg
     7081 accacgtggc gcggcgcggg cacgtacacc ggatacggct tcacgatcac ccgcggcgcc
     7141 ggcacgtagg cgggagccgg cggagcgtag tagacgggcg gcggcggcgc gtagaccacc
     7201 ggcggcgcgg cggccaccac cggaccgcga tggaagatgc cgacgccacc caggatctgg
     7261 ttgaacaccg cgaaggcgcc cagtcccagg gccgcgtccg tggacgcacc cgcgaaggcc
     7321 aggccaggca tcaagaccgc ggccgcgatt accagcgcga tcagcttttt catggggata
     7381 tctcctttcg agagccgtga gccaaccctg ccgtacgccc gtatagacgg agggggggcc
     7441 caccccgttt accccgcccg gctggcgccc gggcgccggt tgccttacca tggcgtccca
     7501 tgagcgaaca cgcgagcgtc tgccccctcg attgcccgga cacctgcagc ctcaccgtga
     7561 ccgtggagca cgcgaagatc gtgaagatcc gcggctcgcg ggccaatccc tacaccgccg
     7621 gggtgatctg caccaaggtc gccggctcg
//
LOCUS       DASUFA010000367         7247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1342289, whole genome shotgun sequence.
ACCESSION   DASUFA010000367 DASUFA010000000
VERSION     DASUFA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342289"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFX87_13500"
     CDS             <1..364
                     /locus_tag="VFX87_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4995991.1"
                     /translation="ERVLGLFPILAERRRQAAGTMSGGQQQMLAIGRALMARPRLLML
                     DEPSLGLAPKIVQEIFAIVAEINRAGTTVLLVEQNTRQALALAGRGYVLENGRVVLSG
                     AGRDLLDNEHVQRAYLGM"
     gene            367..1782
                     /locus_tag="VFX87_13505"
     CDS             367..1782
                     /locus_tag="VFX87_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4995992.1"
                     /translation="MDDLLFSPITELASAVRSRRLSPVELTRAYLDRIAKLDATLRAF
                     ITVDGEGALAAARVLEREAMAGAWRGPLHGVPLAHKDLCLIAGLPTSCGTLTADYFVG
                     APACTAVTRLVAAGALTLGKLNMTELALGPFGDNAHHGDVQNPWRLGHVSGGSSSGSG
                     AAVAAALAAGALGTDTGGSIRLPAACCGVVGLKATYGRVSRTGVMPLSWSYDHVGPLA
                     RTTRDAALMLGLIAGHDPLDATSSRRAVPDYLAALDGAVKGLRVGVAGGFYAEDLDAA
                     VSSALADAVAALRELGATVEPVTVPDPGSLVAACSNPMVRAESAAVHSRLLKERPGDL
                     QPAVRDRLAPGLAVSAYEYLQGQRLRARFTREFIEAVFSRIDVLVTPTIPEPAPSLAN
                     AKAGASAAVIARMGRFSRLTRPFNALGVPALSIPCGVAADGRPLAMQLVGRPFEEATL
                     LRLGDAYERATAWHRRRPPLP"
     gene            1831..3261
                     /locus_tag="VFX87_13510"
     CDS             1831..3261
                     /locus_tag="VFX87_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4995993.1"
                     /translation="MLDLVIRNGQVITPQGVGLWDVGVRGERIAVIGAPGTLPTDGAR
                     VIDAAGQIVSPGGVEPHAHLAHGIMSHPEEPAITLGPEDDTKGMACGGTTTHIDFAYV
                     RPGNDIQPVIEQRASRWKGNSYVDYAFHLTLAGALPVRVFDQIGEAVQAGYPSFKVFT
                     TNVLPPHPKRAGNRIDFGRIHFAMEKVAKAGGIMVVHGEDEDLVQFNYERFREEGRME
                     GSNLHLVHTKLSELLAFRRTTALAKATGAGVYFVHVSAREGVESIAEARAQGLPVYGE
                     TLHQYACFSAEYYKTPRGFCSHTYPSLKFPEDQQALWEGLVHDGLSTLATDEYPTSLE
                     LKLKGRTIEDVTGGNVGAEARIGIGYTEGVVKRGMSLTRFADVTATNAARILGLYPKK
                     GVIAPGSDADLVLIDPTIRKTLTRDDFHVTDYSPWEGWAVSGWPIMTLLRGKIIAERG
                     RLLGSPSDGQWVSRKIDPAVLRRPAC"
     gene            3331..4011
                     /locus_tag="VFX87_13515"
     CDS             3331..4011
                     /locus_tag="VFX87_13515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX4995994.1"
                     /translation="MADYAGQFNPGFRLDPAKAALVVVDMQYASGSRHEGLGRLLKDQ
                     GREAQGTERFDRIEGVLVPTIRSLLDAFRSRGLRVVFLTVGSELPDYSDLPPHMRPMA
                     EAVGNTRGRREHEILDALAPQPHEPVLNKTTMSAFHSSGFERLIRAWGVEQLVFTGVS
                     TNSCVEGTARDAADRGFRCLLVEDGCGAASRQLHEAACHNFQRLLGRVDSSARVIAEL
                     DAARAPAR"
     gene            4053..4505
                     /locus_tag="VFX87_13520"
     CDS             4053..4505
                     /locus_tag="VFX87_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018000685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4995995.1"
                     /translation="MPGKLFEELELGQVYRHQPGRTITESDNVFFSCLTMNPQPLHVD
                     FHAAQKAEFGKPLVNSLLTLGIAVGLSVGETTLGTTVGNLGFEKVEFPKPVFHGDTIY
                     SETEVVEKRESKSRPQWGIVIFEHRARNQHGDVVMTCRRAAMMRRRSA"
     gene            4523..5407
                     /locus_tag="VFX87_13525"
     CDS             4523..5407
                     /locus_tag="VFX87_13525"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX4995996.1"
                     /translation="MRRSLHFVPGGNEKMIAKALTLPADGLILDLEDAVTPDRKAATR
                     PIVRRWLETLDFGGRERWVRMNPVFTDHAEADVAETIAGRPDGYVVPKPRRAEDVRRV
                     TTMLDALEQRHGVPFGSTRLVLIATETPEGLLNIKEIAGASPRIVAVSWGIEDLSAAM
                     GLPRTRDEEGRYLDIPRYARVMCAVAASAVGVEALDTVFTDIPDLDGLRRECREGVAM
                     GFSGKISIHPSQIEVINAEFTPSKADAEEAAALIAAFEEQARRGAGAFAWKGQMMDMP
                     HLTRARKIAERARRAGVL"
     gene            5458..6999
                     /locus_tag="VFX87_13530"
     CDS             5458..6999
                     /locus_tag="VFX87_13530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4995997.1"
                     /translation="MVYALPVGRRPLANQDEARFALLAREAVESHHWLLPRVRGAIYL
                     NKPPLYFWTVAALAKPFGVVNETTAPLASVLAALVALLAVIALGGRLWDASIGLGAAL
                     VLATAPFFYFMSHQVFSDMMMTAWLAWALYFLVTARQVPAPLLRLVAFYLCVAGALAT
                     KGPAALMGLLGALAVVLVEDGWRRGLRWLRLPVGLGILALASLPWLLPYLLQTERSYV
                     KGVVVGHYGDWYFREKTGSRLVELLDNLGRFLPWTVFIPAGIWWWWHDRDPRRRPLLV
                     WTLVVAAAVSFSGEQRARYFLPVLPPLSLLVAEMLGRAPAEPSGRGRRVLLASFAAAA
                     LVAVAGAVVLLGMAPGGGTFTPPPGWPRGVVAALMMVGPPAALGLLAVRRSGVAATGV
                     LALALGGILIVEGCTYPVRDAERSNIRGFTAAVAERMPPGAHILTYLDAGLVYDFYLR
                     QPVHELSRLADLEGRLAAPEPGDMVLIREDRWATMRAAHEVRWQVLLADRVGRTRMVL
                     LGPRR"
     gene            7002..>7247
                     /locus_tag="VFX87_13535"
     CDS             7002..>7247
                     /locus_tag="VFX87_13535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4995998.1"
                     /translation="MAALDLSVLLPAFNEAENLPQLWSELGPVLDALGRAAEVIIVDD
                     GSTDATPAIARALHEHDARVRLLRLAGNAGLSAALDAG"
BASE COUNT          950 a         2619 c         2619 g         1059 t
ORIGIN      
        1 ggagcgcgtg ctcggcctct tcccgatcct ggccgagcgc cggcgccagg ccgccggcac
       61 catgtcgggc ggacagcagc agatgctggc gatcggccgc gcgctgatgg cgcggccgcg
      121 gctcctcatg ctcgacgagc cgtcgctcgg gctcgcgccc aagatcgtcc aggagatctt
      181 cgccatcgtg gccgagatca accgcgcggg cacgaccgtg ctgctggtcg agcagaacac
      241 gcgccaggcc ctggctctgg ccggccgggg ctacgtgctc gagaacggcc gggtggtgct
      301 ctcgggcgcc ggccgcgatc tcctcgacaa cgagcacgtg cagcgcgcgt acctggggat
      361 gtgatcgtgg acgacctgct cttctcgccc atcaccgagc tggcatccgc ggtgcggagc
      421 cggcggctct cgccggtcga gctgacccgc gcgtatctcg accgcatcgc gaagctcgac
      481 gccaccctgc gcgcgttcat cacggtggac ggcgagggcg ccctggccgc cgctcgggtg
      541 ctcgagcgcg aggcgatggc gggcgcctgg cgcgggccgc tccacggcgt cccgctcgcc
      601 cacaaggatc tctgcctgat cgcgggcctg cccacctcct gcggcaccct caccgccgac
      661 tacttcgtgg gcgcgccggc ctgcaccgcg gtgacacggc tcgtggcggc gggcgcgctc
      721 acgctcggca agctcaacat gacggagctg gccctggggc ccttcggcga caacgcgcat
      781 cacggcgacg tgcagaaccc gtggcggctg ggccacgtct ccggcggatc gagcagcggc
      841 tcgggggcgg cggtggccgc ggccctggcc gcgggggcgc tcggtaccga caccggcggc
      901 tcgatccggc tgcccgcggc gtgctgcggg gtggtgggac tcaaggccac ctacggccgg
      961 gtgagccgca cgggcgtcat gccgctgtcg tggtcctacg atcacgtggg cccgctcgcc
     1021 cggaccaccc gcgacgcggc gctcatgctg ggcctgatcg cgggccacga tcccctcgac
     1081 gccacgtcga gccgccgcgc ggtgccggac tatctggccg cgctcgacgg cgccgtcaag
     1141 gggctgcgcg tgggcgtggc cggcggcttc tacgccgagg atctcgacgc ggcggtgtcg
     1201 agcgcgctcg cggacgcggt ggccgcgctc cgggagctgg gcgcgacggt cgagccggtg
     1261 acggtgccgg accccgggtc gctggtcgcc gcgtgtagca acccgatggt gcgcgccgag
     1321 agcgcggcgg tccactcgcg cctcctcaag gagcggccgg gcgacctgca gccggccgtg
     1381 cgcgaccgcc tcgcccccgg cctggccgtg agcgcctacg agtacctgca ggggcagcgg
     1441 ctccgcgccc ggttcacgcg cgagttcatc gaggcggtct tctcccggat cgacgtgctg
     1501 gtcacgccca ccatcccgga gcccgccccc tcgctcgcca atgccaaggc gggcgcgtcc
     1561 gccgccgtca tcgcccgcat gggacgcttc tcgcggctca cgcggccgtt caacgcgctc
     1621 ggcgtccccg ccctctccat tccgtgtggg gtggcggccg acgggcgtcc cctcgcgatg
     1681 cagctcgtgg ggcgtccctt cgaggaggcg acgctgctgc ggctgggcga cgcctacgag
     1741 cgcgccaccg cgtggcaccg gcgccggccg ccgctgccgt gagcgcgcga gcgttgacgg
     1801 ccgcgcgtca cctgggctag gctgcctgtc atgctagatc tcgtgatccg caacgggcag
     1861 gtcatcacgc cgcagggcgt cggtctctgg gacgtgggcg tacgggggga gcggatcgcg
     1921 gtgatcggcg cgcccggcac gctcccgacc gacggcgccc gcgtcatcga cgcggcgggt
     1981 cagatcgtct cgcccggcgg cgtcgagccc cacgcgcacc tggcccacgg aatcatgtcg
     2041 catcccgagg agcccgcgat caccctgggc cccgaggacg acacgaaggg catggcgtgc
     2101 ggtggcacca ccactcacat cgacttcgcc tacgtgcggc cgggcaacga tattcagccc
     2161 gtgatcgagc agcgggcgag ccggtggaag ggcaactcgt acgtggacta cgcgttccat
     2221 ctcacgctcg cgggggcgct gccggtccgc gtcttcgacc agatcgggga ggcggtccag
     2281 gcggggtacc cgagcttcaa ggtcttcacc accaacgtgc taccgcccca tcccaagcgc
     2341 gcgggcaacc gcatcgactt cggacgcatc cacttcgcca tggagaaggt cgcgaaggcg
     2401 ggcggcatca tggtcgtgca cggcgaggac gaggatctcg tccagttcaa ctacgagcgc
     2461 ttccgcgagg aagggcgcat ggaggggagc aacctccacc tcgtccacac caagctctcg
     2521 gagctgctgg ccttccgacg gacgaccgcg ctcgccaagg ccaccggggc cggcgtgtac
     2581 ttcgtccacg tctccgcccg cgagggcgtc gagtcgatcg cggaggcccg ggcgcagggg
     2641 ctgcccgtct acggcgagac gctgcaccag tatgcgtgct tcagcgccga gtactacaag
     2701 actccgcgcg ggttctgctc gcacacgtac ccgtcgctca agttcccgga ggatcagcag
     2761 gcgctctggg aagggctcgt gcacgacgga ctctccaccc tcgccaccga cgagtacccg
     2821 acgagcctcg agctcaagct caagggccgc acgatcgagg acgtgaccgg cggcaacgtg
     2881 ggggcggaag cgcgcatcgg catcggctac accgagggcg tggtcaagcg cgggatgtcg
     2941 ctcacgcgct tcgccgatgt caccgccacc aacgccgcgc ggatcctcgg gctctacccg
     3001 aagaagggcg tcatcgcccc cggcagcgac gcggacctgg tgctcatcga cccgacgatc
     3061 cgcaagacgc tgacccggga cgacttccac gtgacggact acagcccctg ggaaggctgg
     3121 gcggtgtcgg ggtggccgat catgacgctg ctccgcggca agatcatcgc ggagcgcggc
     3181 cggctgctcg gcagcccgag cgacggccag tgggtgtcgc ggaagatcga cccggccgtg
     3241 ctccgccgcc cggcctgcta gctgcccgct acgatgtccg atctcggtgc gagcccctca
     3301 ccccgaccct ctccccaggg gggagaggga gtggccgatt acgctggcca gttcaatccg
     3361 ggcttccggc tcgatcccgc caaggcggcc ctggtggtgg tggacatgca gtacgcgagc
     3421 ggctcgcgtc acgagggcct gggccggctc ctgaaggacc aggggcgcga ggcgcagggc
     3481 accgagcgct tcgaccgcat cgagggcgtg ctcgtgccca ccatccggtc gctgctcgac
     3541 gcgtttcgct cccgcggcct ccgggtggtg ttcctcacgg tgggctcgga gctgccggac
     3601 tacagcgacc tgccgcctca catgcggccg atggccgagg cggtggggaa tacgcgcggc
     3661 cggcgtgaac acgagatcct cgacgcgctg gcgccgcagc cgcacgagcc cgtgctcaac
     3721 aagaccacga tgagcgcgtt ccactcctcg ggcttcgagc ggctcatccg cgcctggggc
     3781 gtggaacagc tcgtcttcac cggtgtgtcc accaactcgt gcgtcgaggg taccgcgcgg
     3841 gacgcggccg accgcggctt ccggtgcctg ctcgtggagg acggctgcgg cgcggccagc
     3901 cggcagctcc acgaggccgc gtgccacaac ttccagcggc tgctcgggcg ggtggactcg
     3961 agcgcgcggg tgatcgcgga gctggacgcc gcccgcgcgc ccgcgcgctg agccgtccca
     4021 accacccagc gtcgggaccg gaggagacga gcatgccggg caagctgttc gaggagctcg
     4081 agctgggaca ggtctaccgg catcagcccg ggcgcaccat caccgagtcg gataacgtgt
     4141 tcttctcctg tctcaccatg aacccccagc cgctgcacgt ggactttcac gcggcccaga
     4201 aggcggagtt cggcaagccg ctcgtcaaca gcctgctgac cctgggcatc gcggtgggcc
     4261 tgagcgtcgg cgagaccacg cttggcacca ccgtgggcaa cctgggcttc gagaaggtcg
     4321 agttccccaa gccggtcttc cacggcgaca ccatctactc ggaaaccgag gtcgtggaga
     4381 agcgggagtc gaagtcgcgg ccgcagtggg gcatcgtgat cttcgagcac cgggctcgca
     4441 accagcacgg cgacgtggtg atgacctgcc ggcgcgccgc catgatgcgg cgccggagcg
     4501 cctgacggcg cggatcctcc gcatgcgacg gtcgctccac ttcgtcccgg gcggcaacga
     4561 gaagatgatc gccaaggcgc tcaccttgcc cgccgacggc ctgatcctgg acctcgagga
     4621 cgcggtgaca cccgatcgca aggcggccac ccggcccatc gtgcggcgct ggctcgagac
     4681 gctcgacttc ggcgggcgcg agcgctgggt gcgcatgaac cccgttttca ccgaccacgc
     4741 ggaagcggac gtggccgaaa ccatcgcggg gcgccccgac ggctacgtgg tgccgaagcc
     4801 gcgtcgggcc gaggacgtcc gccgcgtcac caccatgctc gacgcgctcg agcagcgcca
     4861 cggcgtgccg ttcggctcga cgcggctggt cctgatcgcc accgagacgc ccgagggcct
     4921 gctcaacatc aaggagatcg cgggcgcgag cccccgcatc gtcgccgtct cctggggcat
     4981 cgaggacctc tcggcggcca tggggctgcc caggacccgc gacgaggagg gccgctacct
     5041 cgacatcccg cgctacgcgc gcgtgatgtg cgcggtggcg gcctcggccg tgggcgtcga
     5101 ggcgctcgat acggtcttca ccgacatccc ggacctggac gggctccgcc gcgaatgccg
     5161 tgaaggggtc gccatgggct tcagcggcaa gatctccatt cacccgagcc agatcgaggt
     5221 gatcaacgcg gaattcacgc cgtccaaggc ggacgcggaa gaggcggcgg cgctcatcgc
     5281 cgccttcgag gaacaggcgc gtcggggtgc cggggccttc gcgtggaagg ggcagatgat
     5341 ggacatgccg cacctgaccc gggcccggaa gatcgcggag cgggcccggc gcgcgggggt
     5401 gctctgagcc gcggccgcgc ggcggccctc gccgcgctgc tgctcgcctc ggccctcgtg
     5461 gtgtacgcgt tgccggtggg ccgtcgcccg ctcgccaacc aggacgaggc ccgcttcgcc
     5521 ctgctcgccc gggaagcggt cgagtcgcat cactggctgc tgccccgggt gcgcggcgcg
     5581 atctacctga acaagccgcc gctctacttc tggaccgtcg cggcgctcgc caagcccttc
     5641 ggcgtcgtga acgagaccac cgcgccgctc gcctccgtgc tcgcggcgct cgtcgcgctg
     5701 ctcgcggtca tcgcgctcgg cggccggctc tgggacgcga gcatcgggct cggcgccgcg
     5761 ctcgtgctcg ccaccgcgcc cttcttctac ttcatgtcgc accaggtctt ctccgacatg
     5821 atgatgaccg cgtggctcgc gtgggcgctg tacttcctgg tcaccgcgag gcaggtgccc
     5881 gctcctcttc ttcggctggt cgccttctac ctctgcgtgg cgggggcgct cgccaccaag
     5941 gggccggcgg ccctgatggg gttgctgggg gcgctcgcgg tggtgctggt cgaggacggc
     6001 tggcggcgcg gcctccggtg gctgcggctg ccggtgggcc tcggcatcct cgcgctggcg
     6061 agcctgccgt ggctcctgcc ctatctcctg cagaccgagc ggagctacgt gaagggcgtc
     6121 gtggtcggcc actatggcga ctggtacttc cgcgagaaga ccggctcacg gctcgtggag
     6181 ctgctggaca acctggggag gttcctcccc tggacggtct tcattccggc cgggatctgg
     6241 tggtggtggc acgaccggga tccacggcgc cggccgctcc tggtgtggac ccttgtcgtc
     6301 gcggcggcgg tcagcttctc cggcgagcag cgggcccgct acttcctgcc ggtgctgccg
     6361 ccgctgtcgc tgctggtcgc ggagatgctg gggcgcgccc cggcggagcc gtccggacgg
     6421 ggccggcgtg tgctcctggc gtcgttcgcc gcggccgcgc tcgtggcggt ggcgggggcc
     6481 gtggtcctgc tcgggatggc ccccggcggc gggaccttca cgccgccgcc cggctggccg
     6541 cgcggcgtgg tcgcggcgct gatgatggtt ggccccccgg ccgcgctggg gttgctggcc
     6601 gtccgaagat ctggcgtcgc cgccacgggc gtgctggcgc tagccctcgg cggtatcctg
     6661 attgtcgagg gatgcaccta tccagtacgc gacgccgagc gcagcaacat ccgcggcttc
     6721 accgcggcgg tggccgagcg catgccgccc ggcgcccaca tcttgacgta tctcgacgcg
     6781 gggctcgtgt atgacttcta cctccggcaa ccggtccacg agctgtcccg gctggccgat
     6841 ctggaggggc ggctggcggc gccggagccc ggggacatgg tgctgatacg cgaggatcgg
     6901 tgggcgacga tgcgcgcggc ccacgaggtc cgctggcagg tgctcctggc cgaccgcgtg
     6961 ggccgcaccc ggatggtcct cctggggccg cggcggtgag cgtggccgcg ctcgatctgt
     7021 cggtgctgct tcccgccttc aacgaggcgg agaacctgcc ccagctgtgg tccgaactcg
     7081 gtccggtgct cgacgcgctc gggcgggcgg ccgaggtcat catcgtggac gacggcagca
     7141 ccgacgcgac ccccgcgatc gcgcgggccc tccacgagca cgacgcgcgc gtgcgcctgc
     7201 tgcgcctggc cggcaacgcc ggtctctcgg ccgcgctcga cgccggc
//
LOCUS       DASUFA010000368         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1136402, whole genome shotgun sequence.
ACCESSION   DASUFA010000368 DASUFA010000000
VERSION     DASUFA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136402"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFX87_13540"
     CDS             <1..375
                     /locus_tag="VFX87_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX4995999.1"
                     /translation="FVSGCVASDDQGRTVGGSDIVAQTRQVHENIKRCLAAAGATFAD
                     VCKVTVFLKNVAHREAVNTVRKEYFGKSRPASTLIEISQFVRPDLLIEIEATAVLPEK
                     NAGAPPRKARASAPARKPRRRR"
     gene            complement(380..2044)
                     /locus_tag="VFX87_13545"
     CDS             complement(380..2044)
                     /locus_tag="VFX87_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX4996000.1"
                     /translation="MNRIWQRYWPPGLDEARIRLPGDPITTFLRAHAARTPRKAAIVF
                     YGREITFAELEDASDRFAGWLRARGIGPGDRVALYLENCPQFAIAHFGALKAGAITVP
                     LNPMHKAIELQHELEDSGARVLVAAHAGRALVESVRAQTPLEALALVSYRDYLPQQPS
                     LPLPPSLLEEPTGAVGGPAAGVELFSAIAAAPARLQSSEPRAQHDTALIQYTSGTTGA
                     PKGAELTHANITANCELMRAFFAFTSADVVLSVVPWFHITGMEVQLNMMAYTGATLVA
                     LHRFDVETALRAIQQHRCTITTLIATVNVAIVNHPKTPEYDLGSLRLCTSGGAPVPPE
                     IARRWEAITGRPLVEGYGLTETTAPSHSNPPHRPRYGTVGVPLPFTGVRIVSLEDGVT
                     ELPLGESGEITVRGPMVTKGYWRRPDATAEAIQDGWLRTGDIGRVDEEGYFTIEERKK
                     DMIKASGYSVFPAEVEAIMYRHPAVAEVGVVGVPDAYRGEDVLAFVVRRPEAGATERE
                     IVEWCRAQMAVYKAPRAVRFVPDLPKTASGKLLKRALREQARGGAG"
     gene            2109..>3485
                     /locus_tag="VFX87_13550"
     CDS             2109..>3485
                     /locus_tag="VFX87_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4996001.1"
                     /translation="MMRRGLVALVLLVVAAPARAQDAERLDPVVVTATRTEQRLGEAP
                     ASVSVLTREDISRTAAQSLDDLLRQVPGFSLFRRSSSLVAHPTTQGVSLRGIGPSGAS
                     RALVLLDGVPINDPFGGWVYWDRVPLQSVEQVEVVRGGGASVWGNYALSGVINIITRR
                     PTERAAFFEGSYGTGDTGHADLLLTEALGPFRLSLEGTYFTTDGYKVVNASRRGAIDV
                     DAGSEHVTFNGRAELVLSPDLSVFVRGTYYEESRDNGTRLQINNTDSGSFATGVRART
                     GDGSEWTGTVFADFQAFRSTFSTQAADRNSETLALDQRVPSTSVGAALSWSRTFGDHR
                     LLAGTDFRWIDGETDERVYVAGNFARTRVAGGEQQIYGLFVQDVYRPIPILELVAGIR
                     GDYWRAYDGFRRDTPPPAGIPARQTYSDIDRIGVSPRVAALVHATPTTDVRASAYQGF
                     RVPTINELY"
BASE COUNT          489 a         1267 c         1248 g          481 t
ORIGIN      
        1 ttcgtctccg gctgcgtggc cagcgacgac caggggcgca cggtgggcgg cagcgacatc
       61 gtggcccaga cccgccaggt ccacgagaac atcaagcgct gcctggccgc cgcgggcgcc
      121 accttcgccg acgtgtgcaa ggtgaccgtg ttcctcaaga acgtggcgca ccgggaggcg
      181 gtcaatacgg tgcggaagga gtacttcggc aagagccggc ccgcgagcac cctgatcgag
      241 atctcgcagt tcgtgcgccc ggacctgctc atcgagatcg aggcgacggc ggtcctcccc
      301 gagaagaacg cgggcgcgcc gccgcgcaag gcgcgcgcga gcgcgccggc ccggaagccg
      361 cgccgccggc gctgaggcct cagccggcac caccgcgggc ctgctcgcgc agcgcgcgct
      421 tgaggagctt gccggaggcg gtcttgggca ggtcgggcac gaaccgcacc gcgcgcggcg
      481 ccttgtagac cgccatctgg gcgcggcacc actcgacgat ctcgcgctcg gtggcgccgg
      541 cctccgggcg ccgcaccacg aaggcgagca cgtcctcccc gcggtaggcg tcgggcacgc
      601 ccaccacgcc gacctccgcc accgcggggt gccggtacat gatcgcctcc acctcggcgg
      661 ggaacacgct gtagcccgag gccttgatca tgtccttctt gcgctcctcg atcgtgaagt
      721 agccctcctc gtccacgcgg ccgatgtcgc cggtgcggag ccacccgtcc tggatcgcct
      781 ccgcggtcgc gtcgggccgc cgccagtagc ccttggtgac catgggcccg cgaacggtga
      841 tctcgcccga ctcgcccagg ggcagctcgg tcacgccgtc ttccaggctg acgatgcgca
      901 cgccggtgaa cggcagcggc accccgaccg tgccgtagcg cgggcggtgc ggcgggttcg
      961 aatggctggg cgcggtggtc tcggtgagcc cgtaaccctc cacgagcggc cgcccggtga
     1021 tggcctccca tcgccgcgcg atctcgggcg gcaccggcgc gccgcccgac gtgcagagcc
     1081 gcagcgagcc caggtcgtac tcgggcgtct tcgggtggtt cacgatcgcc acgttgacgg
     1141 tggcgatcag ggtggtgatc gtacagcggt gttgctggat cgcccgcagc gcggtctcca
     1201 cgtcgaagcg gtgcagcgcc accagggtcg cgccggtata ggccatcatg ttgagctgca
     1261 cttccatccc ggtgatgtgg aaccacggca ccacggagag caccacgtcg gcggaggtga
     1321 acgcgaagaa cgcgcgcatc agctcgcagt tcgcggtgat gttggcgtgc gtcagctcgg
     1381 cccccttggg cgcgcccgtc gtgcccgacg tgtactggat cagcgcggtg tcgtgctggg
     1441 cgcgcggctc cgacgactgc agccgggccg gcgccgccgc gatcgcggag aacagctcga
     1501 cgcccgccgc gggtcccccc accgcgccgg tcggctcctc gagcagggat gggggcagcg
     1561 gcaggctcgg ctgctggggc aggtagtcgc gatacgagac cagcgccagc gcttcgagcg
     1621 gcgtctgcgc gcggaccgat tcgacgagcg cccggcccgc gtgggcggcc acgagcacgc
     1681 gggcacccga atcctccagc tcgtgctgga gctcgatggc cttgtgcatg ggattgagcg
     1741 ggacggtgat ggcgcccgcc ttgagcgcgc cgaagtgggc gatcgcgaac tgggggcagt
     1801 tctccaggta gagcgcgacc cggtcgcccg gcccgatgcc gcgggcgcgg agccagcccg
     1861 cgaaacgatc gctcgcgtcc tcgagctcgg cgaaggtgat ctcacgtccg tagaagacga
     1921 tggccgcctt gcgcggggtg cgcgccgcgt gggcccggag gaacgtggtg atcgggtcgc
     1981 cgggaagccg gatgcgggcc tcgtcgaggc cgggcggcca gtacctctgc cagatccggt
     2041 tcatggtccc gtgccggata ctacacgcgt ttgacacccg gtgacgcgcc gggtagcatc
     2101 ccccgacgat gatgcgccgg ggcctcgtcg cactggtcct gctggtcgtg gccgcccccg
     2161 cgcgcgcgca ggacgccgag cggctcgacc cggtggtggt caccgcgaca cgcaccgagc
     2221 agcgtctcgg tgaggcgccg gccagcgtca gcgtgctcac ccgcgaggac atcagccgga
     2281 ccgcggccca gagcctcgac gacctcctgc ggcaggtgcc gggcttcagc ctcttccgcc
     2341 gctcctccag tctcgtcgcg caccccacga cccagggcgt ctcgctgcgc ggcattggcc
     2401 cgagcggggc gagccgcgct ctcgtgctgc tcgacggtgt cccgatcaac gatccgttcg
     2461 ggggctgggt gtactgggac cgcgtcccgc tgcagagcgt ggagcaggtg gaggtcgtgc
     2521 gcgggggcgg cgcgagcgtg tggggcaact acgcgctcag cggcgtgatc aacatcatca
     2581 cgcggcggcc caccgagcgc gcggccttct tcgagggcag ctacggcacc ggcgacaccg
     2641 ggcacgccga cctgctgctg accgaggccc tcggcccctt ccgcctgagc ctcgagggca
     2701 cctacttcac gaccgacggc tacaaggtgg tgaacgcctc gcgccggggc gccatcgacg
     2761 tggacgcggg ctccgagcac gtcacgttca acgggcgcgc ggagctcgtc ctctcgccgg
     2821 atctctcggt gttcgtgcgc ggcacctact acgaggagag ccgggacaac ggcacccggc
     2881 tacagatcaa caacaccgac tcgggctcgt tcgcgaccgg ggtgcgcgcg cgcaccggcg
     2941 acggcagcga gtggaccggc accgtcttcg ccgacttcca ggccttccgc agcaccttct
     3001 ccacccaggc cgcggaccgg aactcggaga ccctcgcgct cgaccagcgg gtgccgagca
     3061 cgtcggtggg cgccgcgctg tcgtggagtc gcacgttcgg agaccaccgg ctgctcgcgg
     3121 gcaccgactt ccggtggatc gacggagaga ccgacgagcg ggtgtacgtg gcgggcaact
     3181 tcgcccgcac gcgggtggcg ggcggcgagc agcagatcta cgggctcttc gtgcaggacg
     3241 tctaccggcc catcccgata ctcgagctgg tcgcgggcat ccgcggcgac tactggcgcg
     3301 cctacgacgg cttccggcgc gacaccccgc cgcccgccgg catccccgcc cgccagacct
     3361 actcggacat cgaccggatc ggcgtgagcc cgcgcgtcgc cgcgctcgtg cacgccacgc
     3421 ccaccaccga cgtgcgcgcc tccgcctacc agggcttccg ggtgccgacg atcaacgaac
     3481 tgtac
//
LOCUS       DASUFA010000369         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1007287, whole genome shotgun sequence.
ACCESSION   DASUFA010000369 DASUFA010000000
VERSION     DASUFA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007287"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..867)
                     /locus_tag="VFX87_13555"
     CDS             complement(220..867)
                     /locus_tag="VFX87_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX4996002.1"
                     /translation="MDAEVREKIHLEILKRTGAYHANDHVLLPSGHHTSEYIEKTLVT
                     TEASFTEGLGAVIAKHFAQWPIDVVLSTGPGALILSHCVARAHPSRPTLAYGTKGVSA
                     GRRRVSLPVEFQRFIRAGTKVLIVEDLVSSGTTVRLLSQLVEELGGQVIGIGALWRRT
                     KKSVIDGKEIFSLVSRDFPTYTTETCPLCKKGVPLNQEYVKRREHRKPSSAHEKS"
     gene            970..1653
                     /gene="tsaB"
                     /locus_tag="VFX87_13560"
     CDS             970..1653
                     /gene="tsaB"
                     /locus_tag="VFX87_13560"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX4996003.1"
                     /translation="MRLLALETATLAGGAALLDDGRLVGESRLNIALTHSERLMAVVD
                     RLLQDCGWDVASLDALAVSIGPGSFTGLRVGAATAKGLALALEIPVAPVPTLDALAAT
                     LPFADAPVCPLLAARRDEVYCSLYRWTGSTMERQWDYLALPPEAVAARLDAPVIVLGD
                     GVPACRPFLGRLGPGLREADAVHSLPSPAAVGALGQAIIAAGRGIPAERLAPLYVRPS
                     EAELKARHA"
     gene            1646..2152
                     /gene="rimI"
                     /locus_tag="VFX87_13565"
     CDS             1646..2152
                     /gene="rimI"
                     /locus_tag="VFX87_13565"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX4996004.1"
                     /translation="MRETPPAHVTLEPMTLEDLDDVLVIERASFQTPWSRGAFRYELT
                     QNRVARCSVARWHGRLVGYLCLWEIGHEIHVTNLATHPDVRRHGVARALLGAVLDDAR
                     ERAVTLAFLEVRPTNSEALGLYERFGFRVIGRRKGYYFDTGEDALVMEARLSGEPSEP
                     AANRRPGA"
     gene            2167..3132
                     /gene="meaB"
                     /locus_tag="VFX87_13570"
     CDS             2167..3132
                     /gene="meaB"
                     /locus_tag="VFX87_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009352943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX4996005.1"
                     /translation="MTDLVRRMLEGDRLALARLITRVENRLPDVPDIMRAVHARTGRA
                     YVLGITGPPGAGKSTLVDRVTSLLRAEQVPVGVIAVDPSSPFTGGAVLGDRIRMQTHT
                     LDPDVFIRSMATRGSLGGLARATGDVIKLMDAFGFPWIIIETVGVGQTELDIIRQADT
                     TVVALVPESGDSIQAMKAGLMEVADIFVVNKADRDGAHALMAELRFSVHLHHTSGAGP
                     RDVDWEVPVLAAQAANDVGIGALLEQVRKHRATLEQAGALEKRRQARRRAELEALLVE
                     EFSAQITARVQTDPALARTLEAVTAGALDSYSAVAQILAQALKRP"
BASE COUNT          420 a         1141 c         1164 g          507 t
ORIGIN      
        1 aatggcgcct tacttcttct ttttcttctt cgcggccttc ttcttcgcca tcgtgtcacc
       61 cccctcccga tccccgcgcg tccggcggtc gatctgtccg gactctaggg gagggtcaaa
      121 agggtgtcaa ggacaaaata ccggatggag gggtcataac ccgcagcagc ccccatatct
      181 tgggggcggg cacggctagg tcctctcgtg cgcgaccggc tagctcttct cgtgcgcgga
      241 agacggcttg cgatgctcgc ggcgcttcac gtactcctgg ttgagcggca cgcccttctt
      301 gcagagcggg caggtctcgg tcgtgtatgt ggggaagtcg cggctcacca ggctgaagat
      361 ctccttgccg tcgatcaccg acttcttggt gcggcgccac agggcgccga tgccgatcac
      421 ctggccgccc agctcctcca cgagctggga gagcagccgc accgtggtgc ccgagctcac
      481 caggtcctcc acgatgagca ccttggtccc ggcgcggatg aagcgctgga actcgacggg
      541 caggctcacc cgtcgcctgc ccgcgctgac ccccttggtg ccgtaggcca gagttggccg
      601 ggaggggtgc gcgcgggcca cgcagtgcga caggatcagg gcgccgggcc cggtggagag
      661 cacgacgtcg atgggccact gcgcgaagtg cttggcgatg accgcgccga ggccctcggt
      721 gaaggaggcc tcggtggtga ccagggtctt ctcgatgtac tcgctggtgt gatgccccga
      781 cggcagcagg acgtgatcgt tggcgtggta ggcgccggtg cgcttgagga tctcgaggtg
      841 gatcttctcc cgtacctcgg cgtccatctg gctgtcggat tgtcgcggcg gcggggccat
      901 cagtcgttca aggatgacgg ccggcgcgcg gcgtgtcaac tcgaaagtgg gcgtgctaga
      961 ctgccgcgca tgaggctgct ggcgctcgag accgccacgc tcgcgggcgg ggccgcgctg
     1021 ctcgacgatg ggcgcctcgt cggcgagagc cggctcaaca tcgccctcac gcattccgag
     1081 cggctcatgg ccgtggtgga ccgcctgctc caggactgcg ggtgggacgt ggcgagcctg
     1141 gacgcgctcg cggtgtccat cgggccgggc tcgttcacgg gcctgcgggt cggcgcggcc
     1201 accgccaagg ggctggccct ggcgctggag attccggtgg cgccggtgcc gacgctcgac
     1261 gcgctcgccg ccacgctgcc cttcgcggac gcgccggtct gtccgctgct cgccgcgcgc
     1321 cgcgacgagg tgtactgctc gctctaccgc tggaccggct cgaccatgga gcgacagtgg
     1381 gactacctgg cgctgccgcc cgaggcggtg gcggcgcgcc tcgacgcccc ggtgatcgtg
     1441 ctgggcgacg gggtcccggc ctgccggccc tttctcggcc ggctggggcc cggcctgcgc
     1501 gaggccgacg cggtccattc attgccgtcg ccggcggcgg tgggcgcgct gggccaggcg
     1561 atcatcgcgg cgggccgcgg catcccggcg gagcggctgg ctccgctcta cgtgcggccc
     1621 tccgaggccg aattgaaggc ccggcatgcg tgagacgccg cccgcgcacg tgacgctcga
     1681 gccgatgacg ctcgaggacc ttgacgacgt gctggtgatc gagcgcgcgt cgttccagac
     1741 gccgtggtcc cgcggcgcgt tccgctacga gctgacccag aaccgcgtcg cgcggtgctc
     1801 ggtggcccgc tggcacggcc ggctcgtcgg ctacctctgc ctctgggaga tcggccacga
     1861 gatccacgtg accaacctgg cgacgcatcc cgacgtccgc cgccacggcg tggcgcgcgc
     1921 gctactgggc gcggtgctcg acgacgcccg ggagcgcgcg gtgaccctgg ccttcctcga
     1981 ggtgcggccc accaactcgg aggccctcgg gctctacgag cgcttcggct tccgggtcat
     2041 cggccggcgc aagggctact acttcgacac cggcgaggac gccctggtga tggaggcccg
     2101 cctgagcggg gagccgtccg agcccgccgc caaccggcgg ccgggcgcct gagctcctcg
     2161 cggcccgtga ccgacctcgt ccgccggatg ctcgagggcg accgtctggc gctggcgcgc
     2221 ctgatcacgc gggtcgagaa ccggctcccc gacgtgcccg acatcatgcg cgcggttcac
     2281 gctcgcaccg gccgcgccta cgtgctgggc atcaccggac cgcccggcgc cggcaagtcc
     2341 acgctggtgg accgggtgac gagcctgctg cgcgcggagc aggtgccggt cggcgtgatc
     2401 gcggtcgatc cctcgagccc gttcacgggc ggcgcggtgc tcggcgaccg catccgcatg
     2461 cagacccaca cgctcgatcc cgatgtcttc atccgtagca tggccacgcg cggcagcctg
     2521 ggcggtctgg cccgggcgac cggcgacgtg atcaagctca tggacgcctt cggcttcccg
     2581 tggatcatca tcgagaccgt cggggtgggc cagaccgagc tcgacatcat ccggcaggcg
     2641 gacaccaccg tggtcgcgct ggtgccggag tcgggcgact cgatccaggc catgaaggcg
     2701 gggctgatgg aggtggccga catcttcgtg gtcaacaagg ccgaccgcga cggcgcccac
     2761 gccttgatgg ccgagctgcg cttcagcgtg catctccacc acacgagcgg cgccgggccc
     2821 cgggacgtgg actgggaggt gccggtgctg gccgcccagg ccgccaacga cgtggggatc
     2881 ggcgcgctgc tcgagcaggt gcggaagcac cgcgccacgc tcgagcaggc gggcgcgctc
     2941 gagaagcggc gccaggcgcg ccggcgcgcc gagctggagg cgctgctggt cgaggagttc
     3001 agcgcgcaga tcaccgcgcg cgtgcagacc gaccccgccc tcgcgcggac gctcgaggcg
     3061 gtgaccgcgg gcgcgctcga ttcctactcg gcggtggccc agattctcgc ccaggccctc
     3121 aagcggcctt gacacccccg agaccccact ccactatgct gtccatgagg cgcccctgat
     3181 gctgccttcg cacacctggt acggcgtcgt tcccggttgg ctgatcttct ac
//
LOCUS       DASUFA010000370         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1228401, whole genome shotgun sequence.
ACCESSION   DASUFA010000370 DASUFA010000000
VERSION     DASUFA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228401"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFX87_13575"
     CDS             <1..612
                     /locus_tag="VFX87_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996006.1"
                     /translation="GEPFHRCTHTALSPQGDPYVSDGYGNARVHKFSPKGTLQFSWGA
                     PGTDPGQFNIAHNICCDADGWVYVADRENHRVQVFDGNGKYQTQWNNMGRPSGLHLER
                     GAQGRFYVGEVGGGVAVNLDVPNIGPRVSVYSHRGELLARLGDRPAGLEPGQFVSPHG
                     LAVDSRGDIYVGEVSYTNWGNRYKGQPHPPGLRSLQKLVKEAT"
     gene            609..1238
                     /locus_tag="VFX87_13580"
     CDS             609..1238
                     /locus_tag="VFX87_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:651..653,aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX4996007.1"
                     /translation="MTTPITLQVFSDYVUPWCYLSTVRIEKLQREHDVKIEWVHFPLH
                     PETPAEGQSLEDLFRGRNVDRKAMHAQMKARMDAEGLPYGDRTMTYNSRLAQELGKWA
                     DTQPGGEAFHDAMFRAYFVEARDISRPDVLLEIAERVGLPVAGAREVLEKRTFKEAVD
                     RDWALSRQYGITGVPTFVAGHRGVVGAQPYEVLEQLVKQARAEEAGEER"
     gene            1240..2166
                     /locus_tag="VFX87_13585"
     CDS             1240..2166
                     /locus_tag="VFX87_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4996008.1"
                     /translation="MLPPPGFHHLHLNSVDPDAAIEFYVRHFPTSTRTRWAGLPALAA
                     PNDVLVLFTRVVAPPATSPQTAIWHFGWHVPDTRASLAVYRARPDLELLPLYTTDEGG
                     SVLVSSDTWPSKGPTPGLTKAQIAEARATGVQPIRTGGFGYMRGPDDALVEYAGNHPA
                     ERFNHVHLYQDDPYCAQLWYQSHLNAPIYAGRTSRTPMTEATCAVPRGPDRSWPALDR
                     DGMFRTPSAAVVFGDVALPWYVRQGDRPLASTRGQLYDHIALSVTDLDAWIAKLRGEG
                     VRFLEEPYRLGDTRAVMIEGPSREALELVEVR"
     gene            complement(2159..2980)
                     /locus_tag="VFX87_13590"
     CDS             complement(2159..2980)
                     /locus_tag="VFX87_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX4996009.1"
                     /translation="MPTTPRPISLAHLTVLDTTPPELVTVAAAAGFRTVGIRLTATPS
                     VGIPPYDVLHEGPMLRETLLRLKDTGVTVLDTEFLRFEPEHPIGIPEGFLEVSARLGA
                     KHVLVMSAEPDEARTLERFGELCDRAAQYGLSVGLEFAVYTGVRTLAHAAQMVAKSKR
                     TNVSVVVDALHFSRSGGVPAHIGQVDAALFRYAQICDASADMPGPTDTPNLIREARTG
                     RLLPGEGVLPLRELVAALPAGIPLAIEAPSRATADLPALERAKRAYRALSALLAT"
     gene            complement(2996..>4192)
                     /locus_tag="VFX87_13595"
     CDS             complement(2996..>4192)
                     /locus_tag="VFX87_13595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996010.1"
                     /translation="SSPRRADVAPPSGKGAEASPWRVLAASTALNAPIGSLYAFSVFL
                     QPLEALLGLTRADLAFVFALAAAGFGVGMMVAPSLYRVASTPLLVAACALANALGLAL
                     AATAGGLTQLAVGYGVLFGVGGGAAYILVQQMVNLAVTRRQGLVNGYIVSLLPAGAMI
                     AAPVFGWAIRAFGVRATLGGFAAVLAVSGLASAWLVARAGVAVSAETGSVALAPAERR
                     RAVFWRLSVVFFLAASAGLMVLSQAAGIIAAYGGAAALAVYGTTFITATIAAARLGGG
                     WMVDWLAIPTVAVGAHAVALAGNAALTLWPGPLVSVFSLALVGMGYGVISGVTAAAVA
                     VYWRRAVYGRVASRVYIAWAAAAIVLPIVAGRLFDVTHGYSTAVLIAAGANVLGILVS
                     LGLPRR"
BASE COUNT          662 a         1534 c         1492 g          504 t
ORIGIN      
        1 ggcgagccgt ttcaccgctg cacgcacacg gcgctgtcgc cgcagggcga tccctacgtc
       61 tccgacggct acggcaacgc gcgcgtccac aagttctcgc cgaagggcac cctgcagttc
      121 tcgtggggcg cgcccgggac cgatccgggc cagttcaaca tcgcgcacaa catctgctgc
      181 gacgccgacg gctgggttta cgtggccgac cgcgagaacc atcgcgtgca ggtgttcgac
      241 ggcaacggca agtaccagac gcagtggaac aacatgggcc gcccctcggg cctccacctg
      301 gagcgcggcg cgcaggggcg cttctacgtc ggcgaggtgg gcgggggcgt cgccgtcaac
      361 ctcgacgtgc ccaacatcgg gccgcgcgtc agcgtctaca gccaccgcgg cgagctgctg
      421 gcgcgcctgg gcgaccgccc ggccggcctc gagccgggcc agttcgtctc gccgcacggc
      481 ctcgcggtgg attcgcgcgg cgacatctac gtcggcgagg tgtcgtacac taactggggc
      541 aatcgctaca agggccagcc ccacccgccc gggctgcgaa gcctgcagaa gctcgtcaag
      601 gaggcgacat gaccacgccc atcacgctcc aggtcttctc cgactacgtc tgaccctggt
      661 gctatctcag taccgtgcgt attgagaagc tccagcgtga gcacgacgtg aagatcgagt
      721 gggtgcactt cccgctccac ccggaaaccc cggcggaagg ccagtcgctc gaggacctgt
      781 tccggggccg caacgtggac cgcaaggcca tgcacgcgca gatgaaggcg cgcatggacg
      841 ccgagggcct tccctacggc gatcgcacca tgacctacaa cagccgcctg gcccaggagc
      901 tgggcaagtg ggcggacacg cagccgggcg gcgaggcctt ccacgacgcg atgttccgcg
      961 cctacttcgt ggaggcgcgc gacatctccc ggccggacgt gctgctcgag atcgcggagc
     1021 gggtgggcct gccggtcgcc ggggctcgcg aggtgctgga gaaacgcacg ttcaaggagg
     1081 cggtggaccg ggactgggcg ctctcgcgcc agtacgggat caccggagtg ccgaccttcg
     1141 tcgcgggcca ccgcggcgtc gtgggagccc agccctacga ggtcctggag cagctcgtga
     1201 agcaggccag ggccgaggag gccggggagg aacgctgaga tgcttccccc gccgggcttt
     1261 caccatcttc atctgaactc ggtcgacccc gacgcggcga tcgagttcta cgtccggcac
     1321 ttcccgacct ccaccaggac gcgctgggcc ggcctgccgg cgctcgcggc accgaacgac
     1381 gtgctggtcc tcttcacccg ggtcgtggcc ccgcccgcca catcgccgca gaccgcgatc
     1441 tggcacttcg gctggcacgt ccccgacacg cgggccagcc tcgcggtcta ccgggcgcga
     1501 ccggacctcg agctcctgcc gctctacacg accgatgaag gcggctcggt cctcgtcagc
     1561 agcgatacgt ggccgagcaa ggggcccacg cccgggctca cgaaggcgca gatcgccgag
     1621 gcccgcgcca ccggcgtcca gccgatccgc accggcggct tcggctacat gcggggcccc
     1681 gacgacgcgc tggtggagta cgcgggcaac cacccggccg agcgcttcaa ccacgtgcat
     1741 ctctaccagg acgatccgta ctgcgcgcag ctctggtacc agagccacct caacgcgccg
     1801 atctatgccg ggcggaccag ccgcacgccg atgaccgagg ccacgtgcgc ggtgccgcgg
     1861 gggcccgatc ggagctggcc cgcgctggac cgcgacggaa tgttccggac gccgtcggcc
     1921 gccgtcgtgt tcggcgacgt ggccctgccc tggtacgtgc gccagggcga ccggccgctc
     1981 gcgagcacgc gcgggcagct ctacgaccac atcgcgctga gcgtcacgga cctcgacgcc
     2041 tggatcgcca agctgcgcgg cgaaggcgtc cggttcctcg aggagcccta ccggctcggc
     2101 gacacgcgcg cggtgatgat cgagggcccg agccgcgagg ccctggagct ggtcgaggtc
     2161 aggtagccag cagggccgac agcgcccggt acgcgcgctt cgcccgctcg agggcgggca
     2221 ggtcggcggt ggcccgggag ggcgcctcga tggccagggg gatgccggcg ggcagcgcgg
     2281 ccacgagctc gcgcagcggc agcaccccct cgccgggcag cagccgcccg gtccgcgcct
     2341 cgcggatcag gttcggcgta tcggtggggc ccggcatgtc ggccgacgca tcgcagatct
     2401 gtgcatagcg gaagagcgcc gcatccacct gcccgatatg agccggcacc ccccccgacc
     2461 ggctgaagtg gagcgcgtcc accaccaccg acacgttcgt ccgcttcgac ttcgccacca
     2521 tctgcgcggc gtgcgcgagc gtgcggactc ccgtgtagac cgcgaactcc aggccgacgg
     2581 acaggccgta ctgggccgcc cggtcgcaga gctcgccgaa gcgctcgagc gtgcgcgcct
     2641 cgtccggctc ggcgctcatg accagcacgt gcttggcacc caggcgcgcc gagacctcca
     2701 ggaatccctc ggggatgccg atcgggtgct cgggctcgaa gcggaggaac tcggtgtcga
     2761 gcacggtcac gccggtgtcc ttcaggcgca gcagcgtctc ccggagcatc ggcccctcgt
     2821 ggagcacgtc gtacggcggg atgcccacgc tcggcgtcgc ggtcaggcgg atgccgacgg
     2881 tccggaagcc cgcggcggcc gcgaccgtga ccagctcggg gggcgtggtg tccagcacgg
     2941 tcaggtgcgc gagcgagatc ggtcgtggcg tggtcggcat gggcgggata gtacctcagc
     3001 gccgcggcag tccgagcgag acaagaatgc cgagcacgtt ggcccccgcc gcgatgagga
     3061 ccgcggtgga gtagccgtgc gtgacgtcga agaggcggcc ggcgacgatg ggcaggacga
     3121 tggcggcggc ggcccacgcg atgtagacgc gactggccac gcgaccgtag accgcccgcc
     3181 gccaataaac ggcgaccgcc gccgcggtca cgccggagat cacgccgtaa cccatcccca
     3241 ccagggccag cgagaagacc gacaccagtg gcccgggcca cagcgtcagc gccgcgttgc
     3301 cggcgagggc gaccgcgtgg gcgccgaccg cgacggtggg aatggcgagc cagtccacca
     3361 tccacccgcc gcccaggcgc gcggctgcga tggtggcggt gatgaacgtg gttccgtaca
     3421 ccgcgagcgc ggccgcgccg ccgtacgcgg cgatgatgcc cgcggcctgg ctcagcacca
     3481 tcaggccggc ggaggcggcc aggaagaaca cgaccgagag ccgccagaac accgcccgcc
     3541 gccgctcggc cggggccagc gcgacactgc cggtctccgc cgagacggcc acgccggccc
     3601 gggcgaccag ccacgcgctc gccaggccgc tcacggccag gaccgccgcg aagccaccga
     3661 gcgtcgcgcg gaccccgaag gcgcggatcg cccagccgaa caccggagcc gcgatcatcg
     3721 cgccggccgg tagcaggctc acgatgtagc cgttcaccag gccctggcgg cgcgtcaccg
     3781 ccaggttcac catctgctgc acgaggatgt aggccgcgcc gcctcccacc ccgaagagca
     3841 ccccgtagcc gaccgcgagc tgggtcagcc cacccgcggt cgcggccaag gccaggccca
     3901 gcgcgttcgc gagcgcgcag gccgccacga ggagcggcgt ggatgcgacg cggtagagac
     3961 tgggcgccac catcatgccg acgccgaatc ccgcggccgc cagggcgaag acgaacgcca
     4021 ggtcggcgcg ggtcagcccg aggagcgcct ccagcggctg caggaacacg ctgaacgcgt
     4081 agagcgagcc gatgggcgcg ttgagcgccg tcgaggccgc gagcacgcgc cacgggctcg
     4141 cctcggcacc cttcccggag ggaggtgcga cgtcagctct ccggggcgag ct
//
LOCUS       DASUFA010000371         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1343369, whole genome shotgun sequence.
ACCESSION   DASUFA010000371 DASUFA010000000
VERSION     DASUFA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343369"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFX87_13600"
     CDS             <1..528
                     /locus_tag="VFX87_13600"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HEX4996011.1"
                     /translation="RVWDLVKIVLVGSILTGLTLGLYYPFLLVKRQAFMISNSYFGSE
                     RFDFAGRGRDLFWVYVQAILLTLPTLGLSWVWYLARKRRYFWDHTSFGPARFSCTVTG
                     RALLGLWAVNALLLVGTLGIAWPWVRVRNIHFAFRNLALLGPLDLARIQQEAQRVSTM
                     GEGLSGFFDSGFDLA"
     gene            550..1644
                     /locus_tag="VFX87_13605"
     CDS             550..1644
                     /locus_tag="VFX87_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX4996012.1"
                     /translation="MRTDWQGSYLDGRTAARHPATIRLMREGLEITTEAGGTRLWRYR
                     ELRQTQGSYAGEVVRLERGGELPETLLVPDAAFLQSLHEAAPHVGPRFHDPRRRSARL
                     RWTIVAAGGVLAATIAIYLWGIPLLAALVTPHVPVAWEETLGRSAVSYLAPPERRCGD
                     PVLGAAMDAIMARLLAAGPPSPYSIRVYVVNRPVVNALAAPGGHVVIFHGLLERTGTP
                     EELAGVIAHELQHILRRHTTRAVIQDVGTGLLLMALTGDVTGPLAYGLETARALGELR
                     YSRRAEEEADVEGMRMLLAARVDPAGLIAFFEKLQKEEGAQPKALTYLSSHPLPGERI
                     ARLKSLAAVRTGAPEPVLPGEDWSALIKRC"
     gene            complement(1748..>2390)
                     /locus_tag="VFX87_13610"
     CDS             complement(1748..>2390)
                     /locus_tag="VFX87_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4996013.1"
                     /translation="MTPLGSVCRPRSYMDALPEDERYAVRAKQGTPVAGVDMRIVDDA
                     GTVLPWDGKSAGEIQVRGPWVTSGYYNNAEGAAQFTEDGWFRTGDVASLDPDGYIQIT
                     DRTKDLIKSGGEWISSVDMETTIMGHPKVLEAAVIAVPHERWVERPLACIVPKPEFKD
                     ALTAAEIMDYLKPRVAKWWLPDEVVFIDAVPKTSVGKFDKKILRERFKNWQPK"
BASE COUNT          305 a          855 c          828 g          402 t
ORIGIN      
        1 cgcgtgtggg atctggtcaa gatcgtcctc gtgggcagca ttctcaccgg cctcaccctg
       61 gggctctact acccgttcct cctggtgaag cgtcaggcgt tcatgatctc gaactcgtac
      121 ttcgggagcg agcggttcga cttcgcgggc cgagggcggg acctcttctg ggtctacgtg
      181 caggcgatcc tgctcacgct gccgaccctc ggcctgagct gggtgtggta tctcgcgcgc
      241 aagcgccgct acttctggga ccacacctcg ttcggccccg cgcgcttctc ctgcacggtc
      301 accggccggg ccctgctggg cctctgggcg gtgaacgcgc tgctgctcgt ggggaccctc
      361 ggcatcgcgt ggccctgggt gcgcgtgcga aacatccact tcgccttccg caatctggct
      421 ctgctgggtc cgctcgatct cgcgcgcatc cagcaggagg cgcagcgcgt ctcgaccatg
      481 ggcgaggggc tctccggctt cttcgactcg ggcttcgatc tcgcctgatc gggggccggt
      541 tccgtcctga tgcgcaccga ctggcagggc agctatctgg acgggcgcac cgcggcgcgc
      601 cacccggcga ccatccgcct catgcgggag ggcctcgaga tcacgaccga ggcgggcggg
      661 acgcgcctct ggcgctaccg cgagctgcgc cagacgcagg gctcctacgc gggcgaagtg
      721 gtgcggctgg agcggggggg agagctgccc gagacgctgc tggttccgga cgccgcgttc
      781 ctccagagcc tgcacgaggc cgcgccccac gtgggtccgc gcttccacga cccgcggcgg
      841 cgctccgcgc gcctgcggtg gaccatcgtg gcggcgggcg gcgtgctcgc ggccaccatc
      901 gcgatctatc tgtggggcat tcccttgctg gccgccctcg tcacgcccca cgtgccggtg
      961 gcgtgggagg agacgctcgg ccggtccgcg gtgtcctacc tggcgccgcc cgagcggcgc
     1021 tgcggcgacc cggtcctggg cgcggcgatg gacgcgatca tggcgcggct cctggccgcg
     1081 ggcccgccgt caccctactc gatacgggtc tacgtggtga accggccggt ggttaacgcg
     1141 ctggccgcgc ccggcggcca cgtggtgatc ttccacggcc tgctcgagcg cacggggacg
     1201 cccgaggagc tggcgggcgt gatcgcccac gagctccagc acatcctgcg gcgccacacg
     1261 acgcgcgcgg tgatccagga cgtgggcacc gggcttctgc tgatggcgct gaccggcgac
     1321 gtgacgggcc cgctcgcgta tgggctcgag accgcccgcg cactgggcga gctccgctac
     1381 tcgcggcgcg ccgaggagga agccgacgtc gagggcatga ggatgctact cgccgcccgc
     1441 gtggaccccg cgggcctgat cgccttcttc gagaagctgc agaaggagga gggcgcgcag
     1501 cccaaggccc tcacgtacct ctcctcgcac ccgttgcccg gcgagcgcat cgcgcgcctc
     1561 aagtccctgg ccgcggtccg gaccggcgcc ccggagcccg tgctccccgg cgaagactgg
     1621 tcggccctca tcaagcgctg ctaggccggg cgctctcggc cggggtcagg tcttacattc
     1681 cgacattcgt ggacggcgga gacggttggg cccgtggaaa tgtcggaatg taagacctga
     1741 ccccaatcta tttcggctgc cagttcttga aacgctcgcg gaggatcttc ttgtcgaact
     1801 tgccgacgct ggtcttgggg accgcgtcga tgaagacgac ctcgtcgggg agccaccact
     1861 tggccacgcg gggcttcaga tagtccatga tctcggcggc ggtcagcgcg tccttgaact
     1921 cgggcttggg cacgatgcag gccagcggcc gctccaccca gcgctcgtgc ggcaccgcga
     1981 tgaccgcggc ctccagcacc ttggggtggc ccatgatggt ggtctccatg tccacgctcg
     2041 agatccactc gccgcccgac ttgatgaggt ccttggtgcg atcggtgatc tggatgtagc
     2101 cgtcgggatc gagcgaggcc acgtcgccgg tgcggaacca gccgtcctcg gtgaactggg
     2161 cggccccctc cgcgttgttg tagtagccgc tggtcaccca cggcccgcgc acctggatct
     2221 cgcccgcgct cttgccatcc cacggcagca ccgtgcccgc gtcgtccacg atcctcatgt
     2281 ccacgcccgc caccggcgtg ccctgcttgg cccgcaccgc gtagcgctcg tcctcgggca
     2341 gcgcgtccat gtaggagcgc ggccggcaca cgctgccgag tggcgtcatc
//
LOCUS       DASUFA010000372         6244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1343538, whole genome shotgun sequence.
ACCESSION   DASUFA010000372 DASUFA010000000
VERSION     DASUFA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343538"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFX87_13615"
     CDS             <1..319
                     /locus_tag="VFX87_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996014.1"
                     /translation="EGWALYALMLLFMVVVFGAIPFVDAMIVQYVDDRMRSRVAGMRL
                     AVSFGVSALAVYLLGPTVKAAGFTTLLLVMAGISCLTTLFVSLLPGRVPSGARAAAPA
                     PAR"
     gene            343..828
                     /locus_tag="VFX87_13620"
     CDS             343..828
                     /locus_tag="VFX87_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4996015.1"
                     /translation="MPDPAFTPGAHRLAEPRWFDDLQLGERFLNPSRTMTEALFAAFQ
                     LASADNHPIHYDREYCHTHGHRELLAHGLLVTAQAAAGAGMFPHGIGESLIAFLEQSS
                     RFLKPVYVGDTVYPALTITALEPGRTTGVVVMRATVHNQAGDLVMEGEHRYLLRRRRP
                     A"
     gene            848..2053
                     /locus_tag="VFX87_13625"
     CDS             848..2053
                     /locus_tag="VFX87_13625"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996016.1"
                     /translation="MARPDTRRWLIVAALFVVTYGISTPLAAYGVFLPVLAEHFGWSR
                     GAISAALSVNLLVGGVAGFGVGALADRRGPRLLLVVTVTLAGAAFALVSVVSALWQLY
                     LLIGLLGGIGMSSFYLLSTATVTHWFDERRGLALALVLVGFNLGYITGGPLAAWLITE
                     LGWRSAYWVLGGGCGLLSFCAAITVRLPHGGERRHLLRAPTGPAGAPPDGMTLRQSLR
                     DPRQWGLNLSWLLLGGLALMISVHAVPFARDQGVSLADASLALTAYGIGSVAGRLTAG
                     FVSDRLGTRATIGAAYGLEIVALIALLWFPSRGALLASLVLFGAGFAASDTMVAKVIP
                     EVFGVRAIGAIMGILTLGWRLGAAIGPAAAGFLYDLTGSYTIPFGAAPVAVALSWLLF
                     SLSTSRARR"
     gene            complement(2060..3211)
                     /locus_tag="VFX87_13630"
     CDS             complement(2060..3211)
                     /locus_tag="VFX87_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX4996017.1"
                     /translation="MVPKLRWIASIVGLWAGVAAAQGARPVETVPGMPPVPDPTNLYS
                     ETRADKLSPAVANALPRVYVPNRQGNDVTVIDPATMKVVDRFAVGINPQHVVPSWDLK
                     TLWVTNNAEGRPDGSLTPIDPATGKPGKTIMIEDPYNMYFTPDGKSAIVVAEALKRLD
                     FRDPQTMAMQYSIEVPQCKGINHADFSIDGRIAIFTCEFQGSIARIDLANRKVLGYLK
                     LSRGGMPQDVRSSPDGTHYFVADMKAGGVFVVDGATFTETAFIKTGIGSHGLYPSRDG
                     TKLYVANRGTSHVYGPPKGKGSVSVIDFATRQVVATWPIPGGGSPDMGNVSVDGKVLW
                     LSGRFDNLVYAIDTTSGEVRSIRVGKEPHGLTVWPQPGRYSLGHTGNMR"
     gene            complement(3225..4022)
                     /locus_tag="VFX87_13635"
     CDS             complement(3225..4022)
                     /locus_tag="VFX87_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX4996018.1"
                     /translation="MRPYILAAALGVLVTAAGAWAQAPDIVLLEELTWTEVRDAVRAG
                     KTTIIIPAGGTEQNGPHMALGKHNARVKALSQDIARQLGDALVAPVLAYVPEGGVAPP
                     TAHMRYPGTITAPEEAFQKVLEFAARSFKQHGFRNIVLLGDHGGTQAGQKAVAARLNR
                     EWAATGVRVHAIEEYYRAGEIEVPRLLRARGYTDAELGRHAGLTDTALMLATDARMVR
                     ADRLRPGSPGDGVDGDPTRATAELGRPGVELIVSRTVDAVRKSITHR"
     gene            4122..5009
                     /locus_tag="VFX87_13640"
     CDS             4122..5009
                     /locus_tag="VFX87_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996019.1"
                     /translation="MEVRSEGVLGTTAVAPHRLRWSRQLRRATHSAVVVAGAVCVALI
                     LLVAGFAGTIAPRDPDDQDFDLIEARPSAQALFGTDRFGRDVLSRVIHGTRISLYVAV
                     TSIAVAMAAGGILGLTAGYLGGAWDNVINRVLDIFFSIPGLLLSVGVAAMRGPGVNSA
                     VIAIAVVYVPLFARVMRGPVLAEREKEYIEAIRALGATRWVVALKHVLPNVISPFVVQ
                     GTVALSQAILIEASLSYLGLSAQPPTPSWGNMLNEGRTYLETAPWISVFPGIAIMITV
                     LAFNLLGDGLRDVLDPGGR"
     gene            5032..>6244
                     /locus_tag="VFX87_13645"
     CDS             5032..>6244
                     /locus_tag="VFX87_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996020.1"
                     /translation="MTNDHGSPRSHLSRRQLLKAGGLTLGALAVGRHAAAQTPKPGGT
                     LVSAQATEATGLDPQLVPALSRSRRSPLMYSQLVRFDDQMTPTPELAESWETSKDGHT
                     WTFKLRQGVKFHDGQEMTSADVKFTFDRLFEKSPGKSDFIAVDKVEPAGKYAVKFVTK
                     EPFAGLLAALGGFWGFIISEAGIKKHGDLNKAALGTGPFMLVDWKVEQQMVLKKNPHY
                     FKKGLPYLDELVLRVIPDEANSVAALRTGQIHHAFIEDNKNYTLLKDEKGLTGYRSSR
                     LGYDFLNINASRGPLKDVRVRQAISWAVDRGQVMRVAASGFGRLTAPATAPMKQWQLP
                     EEQWMRYYKPDVEKAKKLMADAGQAKGFTVKLGVIPTFPTMVSGAPVIAAHLKRIGIT
                     AEIENVEYAVWI"
BASE COUNT          826 a         2295 c         2118 g         1005 t
ORIGIN      
        1 ggagggctgg gcgctgtacg ccctcatgct gctcttcatg gtggtcgtgt tcggggccat
       61 cccgttcgtc gacgccatga tcgtgcagta cgtggacgac cggatgcgct cccgggtggc
      121 cgggatgcgc ctcgcggtct ccttcggggt cagcgcgctg gcggtctacc tcctgggccc
      181 gacggtcaag gcggcgggct tcaccacgct gctgctggtg atggccggca tctcgtgcct
      241 caccacgctc ttcgtctcgc tgctccccgg ccgcgtcccg tcgggcgcgc gcgccgccgc
      301 gcccgcgccg gcgcgctagc gcggccgagc ggaacgcggt cgatgcccga tccggccttc
      361 actccgggcg cgcaccgcct cgccgagcca cgctggttcg acgatctgca gctgggggag
      421 cgcttcctca atccctcgcg caccatgacc gaggccctct tcgccgcctt ccagctcgcc
      481 tcggccgaca accacccgat ccactacgac cgcgagtact gccacaccca cggccatcgc
      541 gagctgctcg cccacgggct cctggtcacc gcgcaggccg cggcgggcgc gggcatgttc
      601 ccgcacggga tcggcgagtc gctgatcgcg ttcctcgagc agtcgtcccg cttcctcaag
      661 ccggtctacg tgggcgacac cgtgtacccc gcgctcacga tcaccgcgct cgagcccggc
      721 cgcaccaccg gggtggtcgt catgcgcgcc accgtgcaca accaggccgg cgacctggtg
      781 atggaaggcg agcaccgcta cctgctgcgg cgaaggcggc ccgcctgatc tccccgatcg
      841 cgcgctgatg gcgcgtccgg acacccgccg ctggctgatc gtggcggcgc tcttcgtggt
      901 cacctacggc atctcgaccc cgctggccgc ctacggcgtc ttcctgcccg ttctcgccga
      961 gcacttcggc tggagccgcg gcgcgatctc ggccgcgctg tccgtcaacc tgctcgtggg
     1021 cggcgtggcc ggcttcgggg tcggcgcgct cgcggaccgg cgcggccccc gcctgctgct
     1081 cgtggtgacg gtcacgctgg ccggcgccgc cttcgcgctc gtctcggtgg tgagtgcgct
     1141 ctggcagctc tacctgctca tcgggctcct gggcgggatc gggatgtcga gcttctacct
     1201 gctgagcacc gcgaccgtca cccactggtt cgacgagcgg cgcgggctcg cgctggccct
     1261 ggtgctcgtc ggcttcaatc tcggctacat cacgggcggg ccgctcgccg cgtggctcat
     1321 caccgagctg ggatggcgca gcgcctactg ggtcctcggc ggcggctgcg ggctgctgag
     1381 cttctgcgcg gccatcacgg tgcgcctgcc gcacggcggc gagcggcgac acctgctgcg
     1441 cgccccgacc gggccggcgg gcgccccgcc ggacggcatg acgctgcgcc aatcgctgag
     1501 ggacccgcgg caatggggcc tgaacctctc ctggctgctg ctcggcgggc tggccctcat
     1561 gatctcggtg cacgcggtcc cgttcgcgcg ggaccagggc gtgagcctgg ccgacgcctc
     1621 gctcgccctg accgcctacg ggatcggctc ggtggccgga cgactcaccg cgggcttcgt
     1681 atccgaccgg ctcggcacgc gcgcgaccat cggggcggcc tacgggctcg agatcgtcgc
     1741 gctgatcgcg ctgctgtggt tcccgtcgcg cggcgcgctg ctcgcctcgc tggtgctgtt
     1801 cggggccggc ttcgccgcct ccgacaccat ggtcgcgaag gtgatcccgg aggtcttcgg
     1861 ggtccgggcc atcggggcca tcatggggat cctgaccctg ggctggcgcc tgggcgccgc
     1921 gatcggcccc gccgcggcgg gcttcctcta cgacctgacc ggctcctaca ccatcccctt
     1981 cggagcggcc ccggtcgcgg tcgcgctgag ctggctcctc ttctccctca gcacctcgcg
     2041 cgcccgccgc tgatcggctt tagcgcatgt tgccggtgtg gcccaacgaa tagcggccgg
     2101 gctgcggcca gaccgtgagc ccgtgcggct ccttgccgac gcggatgctg cggacctcgc
     2161 cgctggtggt gtcgatcgcg tagaccagat tgtcgaagcg cccggagagc cacagcacct
     2221 tgccgtcgac gctcacgttg cccatgtcgg ggctgccccc gcccggaatc ggccacgtgg
     2281 ccaccacctg gcgcgtggcg aagtcgatca ccgagacgct gcccttgccc ttgggcggcc
     2341 cgtacacgtg cgaggtgccg cggttggcca cgtagagctt ggtgccgtcg cggctcggat
     2401 acaggccgtg gctgccgatg ccggtcttga tgaacgcggt ctcggtgaag gtggcgccgt
     2461 ccacgacgaa gacgccgccc gccttcatgt ccgccacgaa gtagtgggtg ccgtcggggg
     2521 agctgcggac gtcctggggc atccccccgc gcgagagctt gagatacccc agcaccttcc
     2581 ggttggcgag gtcgatgcgg gcgatgctgc cctggaactc gcaggtgaaa atggcgatgc
     2641 gcccgtcgat ggagaagtcc gcgtggttga tgcccttgca ctggggcacc tcgatcgagt
     2701 actgcatcgc catcgtctgc ggatcgcgga agtcgagccg cttgagcgcc tccgccacca
     2761 cgatcgcgga cttgccgtcc ggcgtgaagt acatgttgta cgggtcctcg atcatgatgg
     2821 tcttgcccgg cttgccggtg gcgggatcga tgggggtgag gctgccgtcg ggccgccctt
     2881 ccgcgttgtt ggtgacccag agcgtcttga ggtcccacga gggcaccacg tgctgcgggt
     2941 tgatgcccac cgcgaagcgg tccaccacct tcatggtggc cggatcgatc acggtcacgt
     3001 cgttgccctg tcggttgggg acatacacgc gcggcagggc gttcgcgacc gccgggctca
     3061 gcttgtcggc gcgcgtctcg ctgtagaggt tggtgggatc cgggaccggc ggcatgcccg
     3121 gcacggtctc gaccgggcgc gcgccctgcg cggcggccac cccggcccac agcccgacga
     3181 tcgaagcgat ccagcgcagc ttgggaacca tacgtgtcct ctcctcagcg atgcgtgatg
     3241 gatttccgga ccgcgtccac ggtccggctc acgatcagct cgacgcccgg ccgccccagc
     3301 tcggcggtgg cgcgggtcgg gtcaccgtcc accccgtcgc ccgggcttcc gggccgcagg
     3361 cgatccgcgc ggaccatgcg cgcgtcggtg gccagcatca gcgcggtgtc ggtgagcccg
     3421 gcgtggcggc ccagctcggc gtcggtgtac ccgcgggccc ggagcaggcg cggcacctcg
     3481 atctccccgg cccggtagta ctcctcgatc gcgtgcaccc gcacgccggt ggccgcccat
     3541 tcgcgattga gccgcgccgc caccgctttc tgccccgctt gcgttccgcc gtggtcgccg
     3601 agcaggacga tgttccggaa gccgtgctgc ttgaagctgc gcgccgcgaa ctccagcacc
     3661 ttctggaacg cctcctccgg cgcggtgatc gtcccggggt agcgcatgtg ggcggtgggc
     3721 ggcgcgacgc ccccttccgg cacatacgcg agcaccggcg cgaccagcgc gtcgccgagc
     3781 tgccgcgcga tgtcctggga gagcgccttc acccgcgcgt tgtgcttgcc cagcgccatg
     3841 tgcggcccgt tctgctcggt gccgccggcc ggaatgatga tggtcgtctt gcccgcgcgg
     3901 accgcgtcgc ggacctccgt ccaggtgagc tcctcgagga gcacgatgtc cggggcctgg
     3961 gcccacgccc cggcggccgt cacaagcacg ccgagggctg ccgccaggat gtacggtcgc
     4021 atcggaatga tcataatcct taaacaatta acgacccgcc atgtttcccg gctccgtggg
     4081 ccccgcttga caggtggggg cccagagcct agtgttcccc cgtggaagtc aggtccgaag
     4141 gggtgctcgg cacgacggcc gtcgcgccgc accgacttcg atggagccgc cagctccgcc
     4201 gcgccaccca cagcgccgtg gtcgtcgccg gcgcggtctg cgtggccctc atcctgttgg
     4261 tggcggggtt cgcgggcacg atcgcgccgc gcgacccgga cgaccaggac ttcgacctca
     4321 tcgaggcccg gccgagcgcc caggccctct tcggcaccga ccgcttcggg cgggacgtgc
     4381 tgagccgcgt gatccacggc acccgcatct cgctctacgt ggccgtgacc tccatcgcag
     4441 tggcgatggc ggcgggcggc atcctcgggc tcaccgccgg ctacctgggc ggcgcctggg
     4501 acaacgtcat caaccgggtg ctcgacatct tcttcagcat ccccggcctc ctgctctcgg
     4561 tcggcgtggc ggccatgcgc ggccccggcg tcaacagcgc ggtgatcgcg atcgcggtgg
     4621 tctacgtgcc cctcttcgcc cgcgtcatgc gcgggccggt gctcgccgag cgtgagaagg
     4681 agtacatcga ggccatccgc gcgctgggcg ccacccgctg ggtggtcgcg ctcaagcacg
     4741 tgctgcccaa cgtgatctcc cccttcgtgg tgcagggcac ggtggccctg tcgcaggcca
     4801 ttctcatcga agcgtcgctg agctacctgg gcctgtcggc ccagcccccc acgccgtcct
     4861 ggggcaacat gctgaacgag ggacgcacct atctcgagac cgcgccgtgg atctcggtgt
     4921 tcccgggcat cgcgatcatg atcaccgtcc tcgccttcaa cctgctgggc gatggcctgc
     4981 gcgacgtcct cgatcccggc ggccggtaat cccgtcacag gaggaacgac catgaccaac
     5041 gaccacggct cgccccgctc ccacctcagc cggcgccagc tcctcaaggc gggcggcctc
     5101 accctcggcg ccctcgcggt gggccgtcac gccgccgcgc agacgccgaa gccgggcggc
     5161 acgctcgtct cggcccaggc caccgaggcc accggcctcg atccccagct cgtccccgcg
     5221 ctctcgcgca gccgccgctc gccgctgatg tacagccagc tcgtgcgctt cgacgaccag
     5281 atgacgccga cccccgagct ggccgagtcc tgggagacca gcaaggacgg ccatacctgg
     5341 accttcaagc tgcgccaggg ggtgaagttc cacgacggcc aggagatgac cagcgccgac
     5401 gtgaagttca ccttcgaccg cctcttcgag aagagcccgg gcaagtccga cttcatcgcg
     5461 gtggacaagg tcgagccggc cgggaagtac gcggtgaagt tcgtcaccaa ggagcccttc
     5521 gcgggcctcc tcgccgcgct gggcgggttc tggggcttca tcatcagcga ggccgggatc
     5581 aagaagcacg gcgatctcaa caaggccgcg ctcggcaccg ggcccttcat gctggtcgac
     5641 tggaaggtcg agcagcagat ggtcctcaag aagaaccccc actacttcaa gaagggcctg
     5701 ccctacctcg acgagctggt gctccgcgtc atcccggacg aggccaacag cgtggccgcg
     5761 ctccggacgg ggcagatcca ccacgccttc atcgaggaca acaagaacta caccctcctc
     5821 aaggacgaga aggggctgac cggctatcgc agctcgcgtc tcggctacga cttcctgaac
     5881 atcaacgcga gccgcggccc gctcaaggac gtgcgcgtgc gccaggcgat cagctgggcg
     5941 gtggaccgcg gccaggtcat gcgcgtggcc gcctccggct tcggccggct caccgcgccg
     6001 gcaacggcgc ccatgaagca gtggcagctc cccgaagagc agtggatgcg ctactacaag
     6061 cccgacgtcg agaaggcgaa gaagctcatg gccgacgccg ggcaggccaa gggctttacc
     6121 gtgaagctcg gggtcatccc gacgttcccg accatggtgt cgggcgcgcc cgtcatcgcc
     6181 gcccacctca agcgcatcgg catcaccgcc gagatcgaga acgtcgagta cgcggtgtgg
     6241 atca
//
LOCUS       DASUFA010000373         5775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_426206, whole genome shotgun sequence.
ACCESSION   DASUFA010000373 DASUFA010000000
VERSION     DASUFA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426206"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..963)
                     /locus_tag="VFX87_13650"
     CDS             complement(70..963)
                     /locus_tag="VFX87_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996021.1"
                     /translation="MVASELAQYVVTGLLVGGVYALMSIGLALIFGIMRVVNFAQGDF
                     MMLGMYLTFYYAVVLGIDPLLGALLSIPPFFVLGLLVHRAFLVRVTGGGDPNREMDAQ
                     LILTLGLSLVITNATTMILSPMPRSINAPYASKAFAVGPLLLNQARTYSFATALLLAA
                     AVYVFLTRTDLGKALRAAADDPEAAGYQGINVRTMHGVAFGIGIALVAAAGGLLASYH
                     PIEPNVAVNFIVLMFVAVVLGGLGSIPGAFAGGIVIGLVQSLTLLLLPLQLQNVGVFV
                     AFLVVLYVRPQGLFGRRARSV"
     gene            complement(983..2200)
                     /locus_tag="VFX87_13655"
     CDS             complement(983..2200)
                     /locus_tag="VFX87_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996022.1"
                     /translation="MSRLSRSLALTLVLILLAPMVAVPVAVAADDVKIALVAPLSGRW
                     ARQGQLKKMGAEMAIEEINSQGGIKALGGAKIVLREADAGDSVEKAVSAAQRVLTREK
                     ISAGIGSWLSSFTLGVTEVAERLQVPWLTLSYADNITERGFKYTFQTSPVSSLQAEQA
                     LDLVTEIAKKNNRPIKKAALVGDNTAATVFFFKPLREKLLAAKGIELVADEVWTPPLA
                     DATSIVQKLRATQPDLVFYGATNFPDSVQVLQKVKEFNVKTTIQGVGAWLVTPEYVKA
                     IGKENLDGIQTVVAAHPLKGQEALVKKFVQRTGEPFMTQDPLCTYAHVWLIKEAAELA
                     KSADPKAIRDALAKIDLTTGPAATTLYPGRIRFDERGRRVGAIPIIVQWQNGEPYTVV
                     PTAVATRPLASAK"
     gene            2284..3261
                     /locus_tag="VFX87_13660"
     CDS             2284..3261
                     /locus_tag="VFX87_13660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017148670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX4996023.1"
                     /translation="MRALICRAWGPIDGLTVEEIAPPAPAADEVLIDVRATSVNYADS
                     IMVAGKYQTRPPFPFSPGLETAGVVARCGAGATRFKPGDRVMATLAWGGLAEQAVAKE
                     SETFAIPAGMSFDEAGAFPIAYISSDVAIRWQGRLEAGETLLVLGAAGGVGLTAVEIG
                     RAMGARVIAGASTAEKLAVAREHGADEGINYATENLTERVMALTGDRGADVCFDPIGG
                     ELADAALSSLGWGGRMLLVGFVGGVQQIPANRLLVKNRAALGCSLRHYRFNAPDKLRA
                     SVQALLGWYAAGKLKPHVSHRLPLERAVEAIRLLTDRKALGKVVVTLGS"
     gene            3273..4031
                     /locus_tag="VFX87_13665"
     CDS             3273..4031
                     /locus_tag="VFX87_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4996024.1"
                     /translation="MEIIRVDAHGPHVMVVTIDNQPRLNAMSRVMMAELGRIWDELER
                     GSCRAIVLTGAGARAFCAGADISGDLSASPDTARVVGHALLKQDAYTKPIVAAVNGDC
                     VGGGVELLLSTDIRAAAPHARFGLPEVKWSIYPFGGATLKLPQQIGHVHAMDLLLTGR
                     LIDAAEAVRLGLINRIVPAEGLMAWALETAEAIAANSPSAVQAVKRQISSTIADHARG
                     REALEQELGDQVRASAYFREGVAAFREKRRPHYE"
     gene            4074..5123
                     /locus_tag="VFX87_13670"
     CDS             4074..5123
                     /locus_tag="VFX87_13670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996025.1"
                     /translation="MTSPVRVHIRVPGTAPMPELMRLIQSIESAGFDGAGILDSQMIS
                     RDTFVTLGLAATHTRRMQLFPAVTNPFTRHASVLAGAIQTVEELAPGRVKFVMGTGYT
                     SATTIGRKAATLAEMRESIATIKTLLAGESARFGETAGRLGYAGGRRIPVLMAASGPK
                     AIELAGEVADGVLLLVGSNRGIVQRALELLERGARRAGRKVEDLEVIWAVRTAALPTT
                     AEALRQARPTSVHWGILRWGGYWLEPAGLKLPPLRIPDAVWKIYPDLSHAHDWEAAIA
                     ATSFVPDEVVAQLCEAMGLIGTAEDCVARIAELTKLGVQNLYLMPLETFTPPVREIAA
                     FRDTVFPRLAAAGLR"
     gene            complement(5328..>5775)
                     /locus_tag="VFX87_13675"
     CDS             complement(5328..>5775)
                     /locus_tag="VFX87_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX4996026.1"
                     /translation="TPGYYRDPEKNAASFDGDGFLLTGDLGFIGGDGRLRFRGRIKEM
                     IKTGGINVAPLEVEEVLLGHPAVEQAYVVGLPDPRKEEILVAVVVAKPGRDADPVALR
                     ALCRDALAAYKVPQEIRLMRREDLPVTSTGKVQKFRLAEHLAAARS"
BASE COUNT          867 a         1989 c         2085 g          834 t
ORIGIN      
        1 gccagggaga gcacgagccc gatcgcgacc accgccgcga tgccgagcca gccgcggtcg
       61 cgggtcacgc tagacgctgc gggcccgccg gccgaagagg ccctgcggcc ggacgtagag
      121 gacgaccagg aacgccacga acacgcccac gttctggagc tgcagcggca ggagcaggag
      181 ggtcagcgac tgcacgaggc cgatcacgat cccgcccgcg aaggctcccg ggatgctgcc
      241 gagcccgccc aggaccaccg ccacgaacat cagcacgatg aagttgaccg cgacgttcgg
      301 ctcgatcggg tgataggacg cgagcagtcc acccgccgcc gccaccagcg cgatcccgat
      361 cccgaaggcc accccgtgca tggtccgcac gttgatgccc tggtagcccg ccgcctccgg
      421 gtcgtcggcg gccgcgcgca gggccttccc caggtcggtg cgggtcagga agacgtacac
      481 cgcggcggcg agcaggagcg cggtggcgaa ggagtaggtg cgcgcctggt tcaggagcag
      541 ggggcccacc gcgaacgcct tcgaggcgta gggcgcgttg atggagcggg gcatcggcga
      601 gaggatcatc gtcgtcgcgt tggtgatgac gagcgagagg ccgagcgtga ggatgagctg
      661 ggcgtccatc tcgcgattgg gatcgccgcc gccggtgacg cggacgagga aggcgcggtg
      721 cacgaggagg cccaggacga agaagggcgg gatgctcagc agggcgccca gcagcggatc
      781 gataccgagc acgaccgcgt agtagaacgt gaggtacatc cccagcatca tgaagtcgcc
      841 ctgcgcgaag ttgaccacgc gcatgatgcc gaagatgagc gcgagcccga tgctcatgag
      901 ggcgtagacg ccccccacga gcagaccggt cacgacgtac tgcgcgagct cgctcgcgac
      961 cacggcgagc cggcggccgg tgctacttgg ccgacgcgag cgggcgggtg gccaccgcgg
     1021 tcggcaccac cgtgtagggc tcgccgttct gccactgcac gatgatcggg atcgcgccca
     1081 cgcggcggcc gcgctcgtcg aaacggatcc gccccggata aagcgtcgtc gcggccgggc
     1141 cggtggtcag gtcgattttg gcgagggcgt cgcggatggc cttgggatcg gccgacttcg
     1201 ccagctccgc cgcctccttg atcagccaga cgtgcgcgta ggtgcacagc gggtcctggg
     1261 tcatgaaggg ctcgccggtg cgctgcacga acttcttcac cagcgcctcc tggcctttca
     1321 ggggatgcgc ggccaccacc gtctggatgc cgtccaggtt ctccttgccg atggccttca
     1381 cgtactccgg ggtgaccagc cacgcgccca cgccctggat ggtggtcttg acgttgaact
     1441 ccttcacctt ctgcagcacc tggaccgagt cggggaagtt ggtggccccg tagaacacga
     1501 gatcgggctg ggtggcgcgg agcttctgca cgatggaggt cgcgtcggcg agcggtgggg
     1561 tccacacctc gtcggccacc agctcgatgc ccttcgcggc gaggagcttc tcgcgcagcg
     1621 gcttgaagaa gaacacggtg gccgcggtgt tgtcgcccac cagcgcggcc ttcttgatcg
     1681 gccgattgtt cttcttggcg atctcggtca ccagatcgag ggcctgctcg gcctgcagcg
     1741 acgacaccgg cgaggtttgg aaggtgtact tgaacccgcg ctcggtgatg ttgtcggcgt
     1801 aggagagggt gagccacggc acctgcagcc gctccgccac ctcggtcacg cctagggtga
     1861 acgagctgag ccaggagccg atgcccgcgc tgatcttctc gcgggtcagc acgcgctgcg
     1921 ccgcgctcac cgccttctcc acgctgtcgc ccgcgtcggc ctcgcgcagg acgatcttgg
     1981 ccccgccgag cgccttgatg ccgccctggc tgttgatctc ctcgatggcc atctccgcgc
     2041 ccatcttctt gagctggccc tgccgggccc agcggccgga gagcggcgcc accagcgcga
     2101 tcttcacgtc gtccgcggcg accgcgacgg gcaccgcgac catgggagcg aggaggatga
     2161 gaacgagagt gagggcgagg gaacgggaga gacggctcat cggctgatcc tccggcgttg
     2221 gagtgcgtga gagtcctgaa cgagcggcgc ctactatagc acgctgagtt atactccacc
     2281 gcgatgcgcg ccctgatctg tcgtgcgtgg ggaccgatcg acggcctgac cgtggaggag
     2341 atcgccccac ccgcgcccgc cgccgacgag gtgctcatcg acgtgcgcgc cacctcggtg
     2401 aactacgcgg actccatcat ggtggcgggc aagtaccaga cgcgcccgcc gttcccgttc
     2461 agcccggggc tcgagaccgc gggcgtggtg gcgcgctgcg gcgcgggcgc gacgcgcttc
     2521 aagccgggcg accgcgtgat ggccacgctc gcctggggcg ggctcgccga gcaggcggtg
     2581 gccaaggagt ccgagacctt cgcgatcccg gccggcatgt ccttcgacga ggcgggcgcc
     2641 ttccccatcg cttacatctc gagcgacgtg gcgatccgct ggcagggccg gctcgaggcg
     2701 ggcgagacgc tgctggtgct gggcgcggcc ggcggggtcg ggctcaccgc ggtggagatc
     2761 ggccgagcga tgggcgcgcg cgtgatcgcg ggcgcgagca cggccgagaa gctcgcggtc
     2821 gcgcgggagc acggcgcgga cgaggggatc aactacgcga ccgagaacct caccgagcgg
     2881 gtgatggcgc tcaccgggga tcggggcgcc gacgtctgct tcgacccgat cggcggcgag
     2941 ctggccgacg ccgcgctctc ctccctcggc tggggcgggc gcatgctgct ggtcggcttc
     3001 gtgggcggcg tgcagcagat ccccgcgaac cggctgctgg tcaagaaccg cgcggcgctc
     3061 ggctgctcgc tgcgccacta ccgcttcaac gcccccgaca agctccgcgc gtccgtgcag
     3121 gccctgctcg gctggtacgc ggcgggcaag ctcaagccgc acgtgagcca ccggttgccg
     3181 ctcgagcggg ccgtggaggc gatccgtctc ctgaccgatc ggaaggcgct cggcaaggtc
     3241 gtcgtcacgc ttgggagctg accccccctc agatggaaat catccgcgtc gacgcgcacg
     3301 gcccgcacgt gatggtcgtc accatcgaca accagccgcg gctcaacgcg atgtcgcggg
     3361 tgatgatggc ggagctcggg cggatctggg acgagctcga gcgcgggagc tgccgcgcca
     3421 tcgtgctcac cggcgcgggc gcgcgggcct tctgcgcggg ggccgacatc agcggcgatc
     3481 tgagcgcctc cccggacacc gcgcgcgtgg tcggccacgc gctgttgaag caggacgcgt
     3541 acacgaagcc gatcgtggcc gcggtcaacg gggactgtgt gggcggcggc gtggagctcc
     3601 tgctctccac cgacatccgc gcggcggcgc cgcacgcccg cttcggcctg cccgaggtga
     3661 agtggtccat ctacccgttc ggcggcgcca cgctgaagct cccccagcag atcggtcacg
     3721 tgcacgcgat ggacctgctg ctcaccggcc ggctgatcga cgctgctgag gctgtccggc
     3781 ttggcctcat caaccggatt gtccccgccg agggtctgat ggcgtgggcg ctcgagaccg
     3841 cggaggcgat cgcggccaat agcccgtccg cggtgcaggc ggtgaagcgg cagatcagct
     3901 cgaccatcgc cgatcacgcg cgcggccgcg aggcgctcga gcaggagctg ggcgaccagg
     3961 tgcgggcgag cgcctatttc agggaaggag tggccgcatt ccgcgagaag cggcggcccc
     4021 actacgaatg accgggcccc gatcacggag gcccgccaag tgaggagtcc cgaatgacct
     4081 cgccggtccg cgtccatatc cgggtgccgg gcaccgcgcc catgcccgag ctgatgcgcc
     4141 tcatccagtc gatcgagtcg gcgggcttcg acggcgccgg catcctcgac agccagatga
     4201 tctcccgcga caccttcgtc acactcggcc tagccgcgac ccataccagg cggatgcagc
     4261 tcttccccgc ggtcaccaac ccgttcacgc gccacgcctc ggtgctggcc ggcgccatcc
     4321 agacggtgga ggagctggcg cccggacggg tgaagttcgt gatgggcacc ggctatacct
     4381 cggccaccac catcgggcgg aaggcggcca cgctcgcgga gatgcgggag tccatcgcca
     4441 ccatcaagac cttgctggcg ggcgagagcg cgcggttcgg cgagaccgcc gggcgcctgg
     4501 gctacgcggg cggccggcga atcccggtgc tgatggccgc ctccgggccc aaggccatcg
     4561 agctcgcggg cgaggtcgcc gacggcgtgc tgctgctggt gggcagcaac cgcggcatcg
     4621 tgcagcgggc actcgagctc ctcgagcgcg gcgcccggcg cgcggggcga aaggtcgagg
     4681 acctcgaggt catctgggcg gtgcgcaccg cggcgctgcc gaccaccgcg gaggccctgc
     4741 gccaggcgcg gccgacgtcg gtgcactggg gcatcctgcg ctggggcggc tactggctcg
     4801 agcccgcggg cttgaagctg ccgccgctca ggatccccga cgcggtgtgg aagatctacc
     4861 ccgatctctc ccacgcccac gactgggagg cggccatcgc cgccacgtca ttcgtgcccg
     4921 acgaggtggt ggcccagctc tgcgaggcca tggggctgat cggcaccgcg gaggactgcg
     4981 tggcgcggat cgcggagctg accaagctcg gcgtgcagaa tctctacctg atgccgctcg
     5041 agaccttcac gccgccggtt cgcgagatcg ccgcgttccg cgacaccgtc ttcccccgcc
     5101 tcgccgccgc cggcctccgg taggggtcac gacatttcgc gtcccgcgta ggctggggtc
     5161 aggtcttaca ttccaacatt tcgaggccgg tgcgccgctc gaagacggcg attccggcga
     5221 gtggtgacgg aggccaagcc ggcggaggcc gccccggtga caggacccgc ccgcctcgcg
     5281 cgctcgtgtc cctagaaatg tcggaatgta agacctgacc ccagccgtta gctgcgggcg
     5341 gcggcgaggt gttcggcgag gcggaacttc tggaccttgc cggtgctggt gaccgggaga
     5401 tcctcgcgcc gcatgagccg gatctcctgc gggaccttgt aggcggcgag ggcgtcgcgg
     5461 cagagcgcgc ggagcgcgac ggggtcggcg tcgcggcccg gcttcgccac caccaccgcg
     5521 accaggatct cctcttttcg tggatcgggc aggcccacca cgtaggcctg ctcgaccgcg
     5581 gggtggccca gcagcacctc ttcgacctcg agcggggcca cgttgatgcc gccggtcttg
     5641 atcatctcct tgatgcgccc gcgaaagcgc agccgcccgt cgccgccgat gaacccgaga
     5701 tccccggtga gcaggaagcc gtcgccatcg aacgacgccg cgttcttctc gggatcgcgg
     5761 tagtagccgg gcgtg
//
LOCUS       DASUFA010000374         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1592308, whole genome shotgun sequence.
ACCESSION   DASUFA010000374 DASUFA010000000
VERSION     DASUFA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592308"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFX87_13680"
     CDS             <1..487
                     /locus_tag="VFX87_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX4996027.1"
                     /translation="LVLRGAGPEGALAAITPRPVDGTSFTDEGVDNDTDYRYAVRAVR
                     VDPAVSATGEASATVVAAAADTTPPAAPANLLAVPSPGAVRLAWNPSGSSDVALYAIY
                     RAGETGDFMRIATTMAIATVYLDRDVQPGATYRYAVTALDRARRPNESAQSNITSVRV
                     P"
     gene            complement(515..1774)
                     /locus_tag="VFX87_13685"
     CDS             complement(515..1774)
                     /locus_tag="VFX87_13685"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX4996028.1"
                     /translation="MESPRTHRRSAQVTVDVAAEVRRRFGTPCYVYDHVALETAAREA
                     LAFPAPFGLTVRYAMKANPSRGILTLFRDLGLHVDASSDFEVERALTAGFAPERIQLT
                     SQMPSRALERHLKRGVLFNACSLHQLEEFGRIAPGRELSVRVNPGLGTGSTKRTNTGG
                     PASSFGIWHEALGEILAIAKRHDLRIRRLHSHVGSGTDPEVWKRCARMTLDLAAQLPE
                     VATINLGGGFKVGRMPEEPSVDIGDVGAHVRREIEGFHDRAGRGLHLEIEPGTFLVAN
                     AGVIVASCVDVVDTGAEGYCFAKLDAGMPEITRPSLYGAQHPIDVLATGREQKAVVFV
                     GPCCESGDILTPAPGDPEALAPRWVATPRIGDLVVIGGAGAYCAGMATINYNSYPQAP
                     EVMLGRDGTLTLLRRRQAPEQVWQNEV"
     gene            1885..2778
                     /gene="dapA"
                     /locus_tag="VFX87_13690"
     CDS             1885..2778
                     /gene="dapA"
                     /locus_tag="VFX87_13690"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX4996029.1"
                     /translation="MRPTFQGSIVALVTPFRDGKVDEAKLRELVEWHVASGTDGIVPC
                     GTTGESPTLSHDEHKRVVELVIETARGRVAVVAGTGSNSTSEAIDLTAHAKKAGATGA
                     LVVNPYYNKPTQEGLYRHFRAVADAVDIPILVYNIAGRTAVNVETDTLTRIVRDCPNV
                     VGVKEASGSLDQMTQVILACGPDFSVLSGDDNITLPLMSVGGRGVISVIANLVPRETA
                     EMTHAALAGDWKQARELHLKLFPLSRAMFLETNPIPVKEALAMMGKLAEPEFRLPMCR
                     LAEANRPRLRAVLASLGLVKG"
     gene            2792..>3563
                     /gene="dapB"
                     /locus_tag="VFX87_13695"
     CDS             2792..>3563
                     /gene="dapB"
                     /locus_tag="VFX87_13695"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX4996030.1"
                     /translation="MADVVVAGAAGRMGNRLVALLQEDKELRLVAALEAPGHPVLGRD
                     AGEAAGVGRLAVPITADAEAVLGKDRILIEFSVPEASLAHARLAARQGGRAVIGTTGF
                     TAAQREELAAIGRSIAILVAPNMSVGVNVAFRILADMARMLGDDYDVEIVETHHRFKK
                     DAPSGTALRMAEIVAEALGRDLAKTAVYDRHDKLAERSRKEIGMAALRSGDVVGEHTV
                     SFGALGERLELTHRAQSRDNFARGALRAARFIATARPGL"
BASE COUNT          514 a         1292 c         1263 g          494 t
ORIGIN      
        1 tctggtgctg cggggcgcgg gcccggaggg cgcgctcgcc gcgatcacgc cccgtccggt
       61 ggacggcacc tccttcaccg acgaaggcgt cgacaacgac accgactatc gctacgcggt
      121 gcgcgcggtg cgcgtggatc cggcggtcag tgccaccggt gaggcctcgg cgacggtggt
      181 cgcggcggcg gccgacacca cgccgcccgc cgcgcccgcc aacctgctcg cggtgccgtc
      241 cccgggcgcg gtgcggttgg cctggaaccc gagcggctcg tcggacgtgg cgctctacgc
      301 gatatatcgg gcgggcgaga cgggcgactt catgcgcatc gcgacgacga tggcgatcgc
      361 caccgtctac ctggaccgcg acgtgcagcc gggggccacc taccgctacg cggtcaccgc
      421 gctggaccgg gcgcgccgcc ccaacgaaag tgcccagtcc aacatcacct ccgtccgcgt
      481 cccctagaca gggtctccgc ggcgggccgg gcgcctagac ctcgttctgc cagacctgct
      541 cgggggcctg gcgtcggcgc agtagcgtga gcgtgccgtc acgcccgagc atgacctcgg
      601 gcgcctgggg gtacgagttg tagttgatcg tggccatccc cgcgcagtag gcgcccgcgc
      661 cgccgatcac caccaggtcc ccgatccgcg gggtcgccac ccagcggggc gccagcgcct
      721 cgggatcgcc gggggccggc gtgaggatgt cccccgactc gcagcacggc cccacgaaga
      781 ccaccgcctt ctgctcgcgc ccggtggcca gcacgtcgat cgggtgctgg gcgccgtaca
      841 gcgacggccg cgtgatctcg ggcatgcccg cgtcgagctt ggcgaagcag tagccctcgg
      901 cgccggtgtc caccacgtcc acgcaggagg ccacgatcac gcccgcgttg gcgaccagga
      961 aggtgccggg ctcgatctcg aggtgcaggc ctcggcccgc ccggtcgtgg aagccctcga
     1021 tctcccggcg cacgtgcgcg cccacgtcgc ctatgtccac cgacggctcc tcgggcatgc
     1081 ggccgacctt gaagccgccg ccgaggttga tggtcgccac ctcggggagc tgggcggcca
     1141 ggtcgagggt catccgcgcg caccgcttcc acacctccgg atcggtgccg gagccgacgt
     1201 gcgagtgcag gcgccggatg cgcaggtcgt ggcgcttcgc gatcgccagg atctcgccca
     1261 gcgcctcgtg ccagatcccg aagctcgagg cgggcccgcc ggtattggtg cgcttggtgg
     1321 atccggtgcc gaggcccggg ttcacgcgca cggacagctc ccggccgggg gcgatccgcc
     1381 cgaactcctc gagctggtgc agcgagcagg cgttgaagag cacgccgcgc ttcaagtgcc
     1441 gctcgagcgc gcgcgagggc atctgggagg tgagctggat ccgctcgggg gcgaagcccg
     1501 cggtcaacgc ccgctccacc tcgaagtcgc tcgaggcgtc cacgtgcagc cccagatcgc
     1561 ggaacagcgt gagaatgccg cggctcgggt tggccttcat cgcgtagcgc acggtgagcc
     1621 cgaacggcgc cgggaacgcg agcgcctccc gcgccgccgt ctcgagcgcg acgtgatcgt
     1681 agacgtagca gggcgtaccg aaccggcgac gcacttccgc ggcgacgtcg acggtgacct
     1741 gagcggaccg gcggtgggtg cgcgggcttt ccattcgctg gagtctaccg catcggggct
     1801 gccgcttgaa gtgcttccgc ttgtaatgtt agagtcaaat gaactcttcg tccacatcgc
     1861 agtgagcaac ggagggcatc aaccatgcgc ccgacattcc aaggctcgat cgtcgccctc
     1921 gtgaccccgt tccgcgacgg caaggtggat gaggccaagc tccgcgagct ggtcgagtgg
     1981 cacgtcgcca gcggcacgga cggcatcgtg ccctgcggca ccaccggcga gtcgcccacg
     2041 ctcagccacg acgagcacaa gcgcgtggtg gagctagtca tcgagacggc ccgcgggcgg
     2101 gtggcggtcg tcgcgggcac cggctccaac tcgacctccg aggcgatcga tctcaccgcg
     2161 cacgccaaga aggcgggggc caccggcgcg ctggtggtga atccgtacta caacaagccc
     2221 acccaggagg gcctctaccg gcacttccgc gcggtggcgg acgcggtgga catcccgatc
     2281 ctggtctaca acatcgcggg ccgcacggcg gtcaacgtgg agacggacac cctgacccgc
     2341 atcgtgcggg actgcccgaa cgtcgtgggc gtcaaggagg cctcgggctc gctcgaccag
     2401 atgacccagg tgatcctggc gtgcggcccc gacttctcgg tcctctccgg cgacgacaac
     2461 atcaccctgc ccctgatgtc ggtgggcggc cgcggcgtga tctcggtcat cgccaacctc
     2521 gtgccccggg agaccgccga gatgacgcac gccgcgctgg cgggcgactg gaagcaggcc
     2581 cgtgagctcc acctcaagct cttcccgctg tcgcgcgcga tgttcctgga gaccaacccg
     2641 atcccggtca aggaagcgct ggccatgatg ggcaagctgg cggagccgga gttccggctt
     2701 cccatgtgcc gtctggccga ggcgaaccgg ccgcgcctca gagcggtcct cgcctcgctc
     2761 ggcctcgtca agggctgatc ctccactcgt catggcggat gtcgtcgtcg ccggcgccgc
     2821 cggccggatg ggcaaccgtc tggtcgccct gctccaagag gacaaggagc tgcgcctggt
     2881 cgccgccctg gaggctcccg gccaccccgt gctcggccgg gacgcgggcg aggccgcggg
     2941 cgtaggccgg ctcgccgtcc cgatcaccgc cgacgcggag gcggtcctcg gcaaggatcg
     3001 catcctgatc gagttctcgg tgccggaggc gagcctggcc cacgcgcggc tggccgcccg
     3061 gcaggggggc cgtgcggtga tcggcaccac cggcttcacc gccgcgcagc gcgaggagct
     3121 cgcggccatc ggccggagca tcgcgatcct cgtcgcgccc aacatgagcg tgggcgtcaa
     3181 cgtggccttc cgcatcctcg ccgacatggc gcggatgctg ggcgacgact acgacgtgga
     3241 gatcgtcgag acccaccacc gcttcaagaa ggacgctccc agcggcaccg cgctgcgcat
     3301 ggcggagatc gtggccgagg ccctcgggcg cgatctcgcg aagaccgcgg tctacgaccg
     3361 gcacgacaag ctggcggagc gctcccgcaa ggagatcggc atggcggccc tgcgctcggg
     3421 cgacgtggtg ggcgagcaca ccgtctcctt cggcgcgctc ggcgagcggc tcgagctgac
     3481 ccatcgcgcg cagagccggg acaacttcgc gcggggcgcc ctgcgggcgg cccgcttcat
     3541 cgcgacggcc cggcccggtc tct
//
LOCUS       DASUFA010000375         5674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1137974, whole genome shotgun sequence.
ACCESSION   DASUFA010000375 DASUFA010000000
VERSION     DASUFA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137974"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFX87_13700"
     CDS             complement(<1..540)
                     /locus_tag="VFX87_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4996031.1"
                     /translation="MSAPVGFAGRLARAFIDSKLTQLVVLTSLLLGLFAVLATPREEE
                     PQIVVPFADVFVEMPGAAIKEIEERVSIPLEKKLREIPGVEYVYSTSQPGAALAIVRF
                     YVGEDEERSMVKLHDKLRANFDLIPPGASQPLVKPRSIDDVPILALTLWSERADAYTL
                     RRIAAQLDDQVKAVTDVAET"
     gene            complement(537..1742)
                     /locus_tag="VFX87_13705"
     CDS             complement(537..1742)
                     /locus_tag="VFX87_13705"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4996032.1"
                     /translation="MSARSARAMLLLAGLLLLAGCGSERAAEVPGERPAAVSGVRVET
                     VTPAAIRDAVEVAGTVRTRTQMVIASKVQGYVREVRVRQGDHVEHGRVLVTVDERELA
                     ARADRARAVLAEAEMSLDEVKRTLEEAEASLRSAAAERAYAEATASRYRQLWQRELVS
                     AQDYEAVEAKRKSTAAAVEQAEARIQSVRAREKQMSYRIDAGAAELRASEIALGETRI
                     TAPATGVVVDRRVEPGDLAMPGQPLLVLDDPRAYRLEAEVGESAVGRVRVGQRVPVVL
                     DSLGRTLEGRVVEIIPASDPSSRTVTVKLDLPVDPGLRSGLFGRARFPAGERQVLVVP
                     SAALVERGQLTAVYVVDDQGIARLRLVTAGARHADRVEILSGLDAGERVVVEGASRVS
                     DGSRVAAGP"
     gene            complement(1739..2437)
                     /locus_tag="VFX87_13710"
     CDS             complement(1739..2437)
                     /locus_tag="VFX87_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996033.1"
                     /translation="MPAVRFEGLTKIYGSGDTAVVGIQDATMAVERGELVAIIGPSGS
                     GKSTLLTAIGLITEPTSGRIEIDGETIYDNRWVGPDRKRIRRRKMGFIFQHHNLIPFL
                     TVAENVQVALDINGVRGRAAAARVRDLLGYLQIDHRAASYPARISGGERQRVAIARAL
                     ANDPLLILADEPTASLDTERGKSVMDHLRRLAREKGSAVIVVTHDERMIAGFDRIGRV
                     QDGRLLVAPAEVAA"
     gene            complement(2439..3641)
                     /locus_tag="VFX87_13715"
     CDS             complement(2439..3641)
                     /locus_tag="VFX87_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996034.1"
                     /translation="MNLAIRDIRYNLGRFLLTGLGLGLLIAVVMSMAGIYRGLVIEAT
                     TIFNAAGADLWVVQKDTRGPFAESSRIPEDLYRTLAVVPGVQGAAPVSYQNLQVEHAG
                     RPLRLYVVGHRPGGLGGHGPLASGRPIGQKRYEAVADRSAGLGIGEILELGRNRYTVV
                     GLTREMVSSGGDPALFVSLVDAQELQFKKSNEAIRNDRERIEASLRGVTRLAPALAQR
                     ITVTVEAILQGTHIANSVVIRLAPGADAREVARRIEAWKHLTVLTAAEQEQILTRFVI
                     EKARRQLGLFTAILLVISTVIVALIIYTLTIDKIREIATLKLIGAPGFVVVKLIMQQS
                     LALGAIAFAVGALLIHSLYDRFPRRVVLLPADHLALMAIVAGICVLASLLGIRRALAV
                     DPLQALGG"
     gene            complement(3638..4891)
                     /locus_tag="VFX87_13720"
     CDS             complement(3638..4891)
                     /locus_tag="VFX87_13720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4996035.1"
                     /translation="MRKIAVVGAATALLAVAAVVWFRVVGVTAVETVTMGPDEFRAQA
                     FGTGTVEARVYVEVGSKVTGRVVKLLRDQGDTVRQGEILAVLDDEELLRQREQATFVR
                     LKAVESVSLERASLARARASLDARRAAISKALANTELARVTFDRFKQLHDRELIARQD
                     LDVRSTELRATEADVANLRAEASALAADIDRGEVAIRMAEREVAVAGASLAASESRLR
                     ETTVMAPLSGLILSREVEPGAVVVAGVPVFRMVDPRTVWVRIHLDESLLGAVRLGQPA
                     QVTLRSSPGRHFPGEVVRIGEESDRVAEELSVDIRLLEAPPRLRIGEQAEATIITRAA
                     APARVLPASALVHDAAGRPAVFVAENGRAKLRPVTIGERDPRSGAVELTSGIADGERV
                     VVGPAPVPGTLRDGQQIRAARTASR"
     gene            complement(4888..5097)
                     /locus_tag="VFX87_13725"
     CDS             complement(4888..5097)
                     /locus_tag="VFX87_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX4996036.1"
                     /translation="MTLERWIRLIAGSFVTISVVLSYVHSPYWLLFTLFVGLNLFQSA
                     LTRWCLMEDLLKRLGVRSEAAAGRV"
     gene            complement(5367..>5674)
                     /locus_tag="VFX87_13730"
     CDS             complement(5367..>5674)
                     /locus_tag="VFX87_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996037.1"
                     /translation="APKDPTGLMGEVTVPLVEGLHAFAARDWRRVIAKIEPLRPRIVE
                     LGGSRAQRDVFHDTLIEACFRGGDMDRAERVLAERLQRRPDHFWLNRRRAGVAPGSS"
BASE COUNT          813 a         2108 c         1965 g          788 t
ORIGIN      
        1 ggtctccgcc acgtcggtga cggccttgac ctggtcgtcg agctgcgcgg cgatccggcg
       61 cagggtgtac gcatccgcgc gctcgctcca cagggtcagg gccaggatcg gcacgtcgtc
      121 gatcgagcgc ggcttgacga ggggctgcga ggcgccgggc gggatcaggt cgaagttggc
      181 ccggagcttg tcgtggagct tgaccatgct gcgctcctcg tcctccccga cgtagaagcg
      241 cacgatcgcc agcgcggcgc cgggctggct ggtcgaatag acgtactcga cccccgggat
      301 ctcccgcagc ttcttctcca gcgggatgct gacgcgctcc tcgatctcct tgatcgctgc
      361 ccccggcatc tccacgaaga cgtcggcgaa gggcaccacg atctgcggct cctcttcgcg
      421 cggcgtggcc agcaccgcga agagacccag caggagcgag gtcagcacga cgagctgcgt
      481 gagcttggag tcgatgaagg cccgggccag gcggcccgcg aagccgaccg gggcgctcac
      541 ggcccggccg ccacccgcga gccgtcgctc acccgcgaag cgccctccac cacgacgcgc
      601 tcgcccgcat cgaggcccga caggatctcg acgcggtcgg cgtgacgcgc gcccgcggtg
      661 accagcctga gccgggcgat gccctggtca tccaccacgt acacggccgt gagctgtccc
      721 cgctcgacca gagccgcgct cggcaccacg agcacctggc gctccccggc cgggaaccgg
      781 gcgcggccga acagacccga gcgcaatccc gggtcgacgg ggaggtcgag cttgacggtt
      841 accgtccggc tggacggatc ggaggccggg atgatctcaa ccacgcggcc ctccagggtg
      901 cggccgagcg agtcgagcac gaccgggacc cgctggccca cccgcacgcg gcccaccgcc
      961 gattcgccga cctcggcctc gagccggtac gcccgcgggt cgtcgaggac gagcagcggc
     1021 tgccccggca tcgccaggtc ccccggctcc acgcgacgat ccaccaccac gccggtggcg
     1081 ggcgccgtga tgcgcgtctc gcccagcgcg atctcgctcg cccgcagctc ggccgcgccc
     1141 gcgtcgatcc gatagctcat ctgcttctcg cgcgcgcgaa ccgactggat ccgcgcctcg
     1201 gcctgctcga cggccgcggc cgtgctcttg cgcttcgcct cgacggcctc gtagtcctgc
     1261 gcgctgacca gctcgcgctg ccacagctgg cggtatcggc tcgccgtcgc ctcagcgtag
     1321 gcgcgctcgg ccgcggccga gcggagcgag gcctcggcct cttccagtgt ccgcttcact
     1381 tcatcgaggc tcatctcggc ttccgcgagg acggcccgcg cgcggtcggc gcgcgccgcg
     1441 agctcgcgct cgtccaccgt gacgaggacc cggccgtgct cgacgtggtc gccctggcgc
     1501 acccgcacct cccggacgta gccctgcacc ttgctggcga tgaccatctg ggtcctggtc
     1561 cggacggtgc ccgcgacctc cacggcgtcc cggatggcgg ccggcgtcac cgtctccacc
     1621 cggacgccac tcaccgccgc gggccgctct cccggcacct cggcggcgcg ctcgctcccg
     1681 cagccggcga gcaggagcag gccggccagg agcagcatcg cgcgcgccga ccgcgcgctc
     1741 acgccgcgac ctccgcgggc gcgaccagca gccggccgtc ctggacccgg ccgatccgat
     1801 cgaagcccgc gatcatccgc tcgtcgtgcg tcacgacgat gaccgcggag cccttctccc
     1861 gcgccagccg ccgcaaatgg tccatgacac tcttgccgcg ctcggtgtcg agcgaggcgg
     1921 tgggctcgtc ggccaggatg agcagtgggt cgttggccag cgcccgcgcg atggccaccc
     1981 gctgccgctc cccgcccgag atgcgggcgg gatagctggc ggcccggtga tcgatctgga
     2041 ggtagcccag gaggtcccgg acccgggcgg ccgcggcgcg gccgcgcacg ccgttgatat
     2101 ccagcgccac ctgcacgttc tcggcaaccg tgagaaacgg gatcaggttg tgatgctgga
     2161 agatgaagcc catcttgcgc cgccggatgc gcttgcggtc aggcccgacc cagcggttgt
     2221 cgtagatcgt ctcgccgtcg atctcgatcc ggccggaggt gggctcggtg atgagtccga
     2281 tcgcggtcag cagcgtggac ttgcccgagc cgctgggccc gatgatggcc accagctcgc
     2341 cccgttccac cgccatggtg gcgtcttgaa tgcccaccac ggcggtgtcg ccgctgccgt
     2401 agatcttggt gagcccctcg aagcgcaccg ccggcatctc agcctcccag cgcctggagc
     2461 ggatccaccg cgagcgcccg ccggatgcca agcaggctgg ccagcacgca gatgccggcc
     2521 acgatggcca tgagggcgag atggtcggcc ggcaacagca ccacccggcg ggggaagcgg
     2581 tcgtagagcg agtggatcag gagagcgccc accgcgaacg cgatggcgcc cagcgcgagg
     2641 gactgctgca tgatcagctt cacgacgacg aagccggggg cgccgatcag cttgagggtg
     2701 gcgatctccc ggatcttgtc gatggtgagc gtgtagatga tgagggccac gatcaccgtg
     2761 gagatgacga gcaggatcgc ggtgaagaga ccgagctgcc ggcgcgcctt ctcgatcacg
     2821 aaccgggtga ggatctgctc ttgctccgcc gcggtgagca ccgtgaggtg cttccaggcc
     2881 tcgatccgcc gggccacctc gcgggcgtcg gctccgggcg ccagccggat caccaccgag
     2941 ttggcgatgt gggtgccctg gaggatggcc tccacggtca cggtgatccg ctgcgccagc
     3001 gccggggcca gccgggtcac gccgcgcagc gaggcctcga tgcgctcacg gtcgttccgg
     3061 atcgcctcgt tgctcttctt gaactgcagc tcctgggcgt cgacgagcga gacgaagagc
     3121 gccgggtccc cgcccgagga caccatctcg cgggtcagcc ccactacggt gtagcggttc
     3181 cggccgagct cgaggatctc gccgatcccg aggcccgcgc tccggtcggc cacggcctcg
     3241 tagcgcttct gtccgatcgg gcggccggag gcgagcgggc cgtgcccacc gagcccccca
     3301 gggcggtggc ccaccacata gagccggagc ggccggccgg cgtgctcgac ctggaggttc
     3361 tggtacgaga ccggcgccgc tccctgcacg ccgggcacga ccgccagggt ccggtagaga
     3421 tcttcgggaa tgcgggagct ctcggcgaag ggcccccggg tgtccttctg caccacccac
     3481 agatctgctc ccgcggcatt gaagatcgtg gtggcctcga tcaccagccc ccggtagatc
     3541 ccggccatgg acatgacgac ggcgatcagc agccccagcc cgaggcccgt cagcaggaag
     3601 cggccgaggt tgtagcggat gtcccggatc gcgaggttca tcgggacgcc gtccgggccg
     3661 cgcggatctg ctggccatcc ctcagcgtgc ccgggacggg ggccgggccg accacgaccc
     3721 gctcgccgtc ggcgatgccg gaggtcagct ccaccgcacc gcttctcggg tcccgctccc
     3781 cgatcgtcac cggccggagc ttcgcacggc cgttctccgc gacgaacacc gcgggccgcc
     3841 cggccgcgtc gtggaccagc gccgaggcgg gcaagacccg ggcgggcgcg gcggcccgcg
     3901 tgatgatggt ggcctcggcc tgttccccga tgcgaaggcg cggcggtgcc tcgagaagcc
     3961 ggatgtccac gctcagctcc tcggccaccc gatcgctctc ctctccgatc cggaccacct
     4021 cgcccggaaa gtggcggccc gggctactcc ggagcgtgac ctgagccggc tgccccagcc
     4081 ggaccgcgcc cagcaggctc tcgtccagat ggatccggac ccagaccgtt cggggatcga
     4141 ccatcctgaa gacgggcacg ccggccacca ccaccgcgcc gggctcgacc tcgcgtgaca
     4201 ggatgagccc cgagagcggc gccatgacgg tggtctcccg caggcgtgac tcgctggcag
     4261 cgagcgaggc gccggccacg gccacctcgc gctcggccat gcggatcgcc acctcgccac
     4321 ggtcgatgtc ggccgcgagg gccgaggcct cggcgcgcag gttcgcgaca tcggcctcgg
     4381 tcgcgcggag ctcggtcgat cggacgtcca ggtcctggcg cgcgatgagc tcgcggtcgt
     4441 gaagctgctt gaagcgatcg aacgtcacgc gggccagctc ggtattggcc agagccttgc
     4501 tgatggcagc ccgtcgggcg tcgagactgg cccgggcccg ggcgagactg gcccgctcga
     4561 gagagacgga ttcgaccgcc ttgagccgca cgaacgtggc ctgctcgcgc tgccggagca
     4621 gctcctcgtc gtcgagcacg gccaggatct cgccctgccg gaccgtatcg ccctggtcgc
     4681 gcaggagctt cacgacccgc ccggtcacct tgctcccgac ctcgacgtac acgcgggcct
     4741 ccaccgttcc ggtgccgaag gcctgggccc ggaactcgtc cgggcccatg gtcacggtct
     4801 cgaccgcggt gacccccacg acgcggaacc agaccaccgc cgcgacggcc aggagggccg
     4861 tggccgcgcc gacgacggcg atcttcctca tacgcggccg gcggctgcct cgctgcgcac
     4921 cccgagccgc ttgaggagat cttccatcaa gcaccagcgg gtcagcgcag actggaagag
     4981 attgagcccg acgaacagcg tgaacaagag ccagtacggg ctgtgcacgt agctgagcac
     5041 gacgctgatc gtgacgaagc tcccggcgat caggcggatc cagcgctcga gtgtcatggc
     5101 ctttccctcc ccggccgact gcggccgaca aagggaaaat gcaacggagg tgccagtcga
     5161 ggactcactc caagtcaacc acttatggcc acccggccga gggtcacgtg aggccggcgc
     5221 gcctcactcg gcgcggcaac gtcccgcccg ggtgagcgcc cgtcctgctg ggcgtcttgc
     5281 tagccgcggc cggattcgcg ctgatcctcg atcaggtcaa ggtgccggtg ctgtcccggc
     5341 tccggatcga ctgaggtatg gctcgctcag ctcgacccgg gcgccacgcc ggcccggcgc
     5401 cggttgagcc agaagtggtc ggggcgccgc tggaggcgct cggcgagcac ccgctcggcg
     5461 cgatccatgt cgccgccgcg gaagcacgcc tcgatcagcg tgtcgtggaa gacgtcgcgc
     5521 tgggcacggc tgccgccgag ctcgacgatg cgcggccgca gcggctcgat tttcgcgatc
     5581 acgcgccgcc agtcgcgggc cgcgaaggcg tggagcccct ccaccagcgg caccgtcacc
     5641 tcgcccatca gccccgtcgg atccttcggc gctc
//
LOCUS       DASUFA010000376         6846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_94290, whole genome shotgun sequence.
ACCESSION   DASUFA010000376 DASUFA010000000
VERSION     DASUFA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94290"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1119
                     /locus_tag="VFX87_13735"
     CDS             256..1119
                     /locus_tag="VFX87_13735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4996038.1"
                     /translation="MPHVAAVLAALVSLIALGPAWAVEVRVVPKAPRQGDTVLVFVSG
                     TRGAQNVEGSLGVQHLTFFPYGAEFAALAGVDLETKPGKVPWRVGLVDGSGVPRKAAG
                     AVTVRAGGFPVERLTLPGGMVNLDPETERRAANEARRMRTLYDTITPERLWHGSFTQP
                     VASRKPGGGFGSRRIINGQPRMPHSGIDYSADRGTPVVASNRGRVALLGEFFFAGRLV
                     ALDHGLGLYTLYFHLDGVAVTEGQMVERGQTVGTVGTTGRSTGPHLHFAAQLGRARVN
                     PPGLYALPLRD"
     gene            complement(1123..2838)
                     /locus_tag="VFX87_13740"
     CDS             complement(1123..2838)
                     /locus_tag="VFX87_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007627802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4996039.1"
                     /translation="MIDPITLAVIKSALDSIVDEMAYTVIRTARSEIIKDVMDYSAAL
                     CDARGRMIAQAKTIAQHLGAIPDAMASVLAEFTGDLRPGDVVAMNDPYQGGMHIPDVF
                     LFMPIFWDGEVEAFAVVIGHQTDMGGRVPGSNASDSTEIYQEGLRLPPIKLYESGLMS
                     RSVRRIIEKNVRVPDRVLGDLGAQYAACRVGERELGRLYTRYGRDEVRRYTAELLDYA
                     ERLTRAEIARWPQGVYTFTDHLDSDGLSDEPVPLRVAITVHPDGHLTCDWTGSSPQVK
                     AALNSTLSFTKSCTYLSVRSVLRQDVPNNVGVFRCIDVIAPEGSILNPHLPGACAARA
                     LTGYRMLDVMLGALAQALPDRVPAAGEGGNTVLSIGGQTADRRPFVIVDMITGAWGGR
                     PDKDGMEAVTNPSQNMSNTPVEVLEAQHPIRIDEYGFVPDSCGAGRFRGGLGLRRRYT
                     LLNDEATLQLRSDRMRFLPYGLEGGGPARGTRNVLNPDTEARIMPAKFAVVLKRGDTV
                     LHEQPGGGGFGDPFTRDPERVAADARNEKITPEYARREHGVVLEPGTFKVNAEATRAV
                     RERRA"
     gene            complement(2835..4925)
                     /locus_tag="VFX87_13745"
     CDS             complement(2835..4925)
                     /locus_tag="VFX87_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4996040.1"
                     /translation="MSARVGVDIGGTFTDIVLRLDDGTLRVSKVSSTPEDPGLAVVRG
                     LDALLRAAAVPPASVVEVVHGTTVASNTILQKKGARVGLLTTRGFRDVLEIGRVRTPD
                     LFDLTWEKPEPLVERRHRLEVDERIGADGAVVRPLDREGLLAAAGRLVADGADVVAVS
                     FINSYVNPAHERLAESLLAARFPALDVTASYRVLPEIKEYERTSTTVVNAYLLPVMRR
                     YLATLAAGLRGIGVEAPLLVVASNGGVVGAQGAGERPVFAVGSGPAAGVAGAARLGQV
                     ADTPNLIAFDMGGTTAKASLVEDGRVSLTTEYEFREGISTSSRFIKAGGYMLKVPAID
                     IAEVGAGAGSIAHIDDGGLLRVGPESAGAHPGPACYALGGTRPTVTDANVALGYLNPA
                     YLAGGELRIDAPRARAAIRAHVAEPLGLSVEEAAHGIREVANANMARAIRSVTVERGR
                     DPRDFTLVAFGGSGPVHAGDVARALDIRRVLVPVSPGVFTAVGMLASDVEHHFVRAAG
                     GPLDECVLERANARLDEMTDEALVTLAQEGYPREQVRLDAQADLRYVGQASELVIPIP
                     GGRLAADRLPALREAFAREYAATYGDASREELELVNVRVVATGVRPHRLDFRAVRVAA
                     GTPAPAAHRLVSFDRRVRAVETPVRLREAVTASGQPGPLIVESYDSTVVVPPGWAVRR
                     DAVDNLLITFGENA"
     gene            complement(4922..5821)
                     /locus_tag="VFX87_13750"
     CDS             complement(4922..5821)
                     /locus_tag="VFX87_13750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX4996041.1"
                     /translation="MSGPIAIIQADIFTDRPFAGNPAAAVLDADRLDAPAMQRIAAEM
                     LVPGTAFVSASTQAGVDWRLRMFTPKREVGYSGHTALCGTHALVESGRLPGDRVTYDT
                     PSGLLRVDVERGGGRALMWLEPPLPACRPFEGDLGEILDSLGLSRAAVGGWARPAVTP
                     DQDLLLPVRDLAALRALEPDMRRLAGVRAPQHARGVCVVSRETVDPGSRSHCRFFAPH
                     YGIPEDIVTGSVHSSLGVWLLEAGVLPAADGRIVFTAEQGDGLGRPGRLQVELTAKAG
                     RAARVRVGGSAVTVLTGTLRSDA"
     gene            complement(5818..>6846)
                     /locus_tag="VFX87_13755"
     CDS             complement(5818..>6846)
                     /locus_tag="VFX87_13755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX4996042.1"
                     /translation="RLAAVPGSDLTAALLRRVSRSFYLSLAVLPRSVRPTIGLAYLFA
                     RASDTIADTRLIDRMSRIAHLEALRAALADDGRSIAVGATASAQALPAERTLLERLPD
                     CLAAYRALPPADRARVHAVLTTIIEGQIQDLRVFPGEDEGKLAALETREDLDRYTYLV
                     AGCVGEFWTEVHVAHRPRLARWDLPRMRALGTRFGKGLQLTNVLRDVPRDLRQGRCYL
                     PRQDLARWGLEPRDLLDPARAPAARPLLGEWLNTALDHYEAGWEYTFAIPRREARMRL
                     ACAWPLLIGLRTLDLLAASPNWLDPAIPLKVPRVRVYGLMAHSLGTVWSTRALGRQAR
                     RLRERIRL"
BASE COUNT          932 a         2442 c         2591 g          881 t
ORIGIN      
        1 gctcgagcgc aagagctcgg acgacgacga ggagcccccc gcgccggcgg aggcgatcga
       61 gccgtccgag gtcgcggaga tcaaggacga gctgccgccg gcgggcgacc tggtcgccga
      121 gatcgagcag tttctccgcc agcaacgccc caaggacccc tagagccacc ctctcccctg
      181 acgggagagg atggggtgag ggggggcggg gggttgctag ggccctggtc gggcccaggc
      241 gtagaatcgt cagcgatgcc ccacgttgcc gcagtcctcg cggccctcgt ctcgctcatc
      301 gccctcggcc ccgcctgggc ggtggaggtg cgggtggtgc cgaaggcgcc ccggcagggc
      361 gacacggtgc tggtgttcgt gtccgggacc aggggcgcgc agaacgtgga aggcagcctg
      421 ggcgtgcagc acctgacctt cttcccttat ggcgccgagt tcgccgcgtt ggcgggcgtt
      481 gacctcgaga ccaagcccgg gaaggtcccg tggcgggtcg gcctcgtcga cggcagtggc
      541 gtgccgcgca aggcggcggg cgcggtgacg gtcagggcgg gcggcttccc ggtggagcgt
      601 ctcacgctcc ccggcggcat ggtgaacctc gatccggaga cggagcggcg cgcggccaac
      661 gaggcgcggc ggatgcggac gctctacgac acgatcacgc ccgagcgcct ctggcacggc
      721 tccttcaccc agccggtggc gagccgcaag ccgggcgggg ggttcggctc ccggcgcatc
      781 atcaacggcc agccgcgcat gccgcactcc ggaatcgact actcggccga ccgcggcacc
      841 ccggtggtcg cgtcgaaccg gggtcgggtg gcgctgctcg gcgagttctt cttcgcgggc
      901 cgcctcgtgg ccctggacca cggcctgggg ctctacacgc tctacttcca cctggacggc
      961 gtggcggtaa ccgagggcca gatggtggag cgcggccaga cggtgggcac ggtgggcacc
     1021 accggtcgct ccaccgggcc gcacctgcac ttcgcggccc agctcgggcg cgcgcgcgtg
     1081 aacccgcccg gcctctacgc gctccccctc cgcgactagg cgctaggccc gccgctcgcg
     1141 gacggcccgg gtcgcctccg cgttcacctt gaaggtgccc ggctcgagca ctacgccgtg
     1201 ctcccggcgc gcgtactcgg gcgtgatctt ctcgttccgc gcgtcggcgg cgacccgctc
     1261 cggatcgcgg gtgaagggat cgccgaagcc gccgccgccc ggctgctcgt gcagcaccgt
     1321 gtcgccgcgc ttcaagacca ccgcgaactt ggccggcatg atgcgcgcct cggtgtccgg
     1381 gttcagcacg ttccgggtgc cgcgcgcggg cccgccgccc tcgaggccgt aggggaggaa
     1441 gcgcatgcgg tcggaccgga gctggagcgt ggcctcgtcg ttgagcagcg tgtagcggcg
     1501 gcgcaggccg aggccgccgc ggaagcggcc ggcgccgcag gagtcgggta cgaagccgta
     1561 ctcgtcgatg cggatggggt gctgcgcctc cagcacctcc accggggtgt tggacatgtt
     1621 ctgcgagggg ttcgtcaccg cctccatgcc gtccttgtcc gggcggcccc cccacgcccc
     1681 ggtgatcatg tccacgatca cgaacgggcg ccggtcggcg gtctgcccgc cgatcgagag
     1741 cacggtgttg ccgccctcgc ccgccgcggg cacccggtcg ggcagggcct gggccagcgc
     1801 gcccagcatc acgtcgagca tccggtagcc ggtcagcgcg cgcgccgcgc aggcgccggg
     1861 caggtgcggg ttcaggatgc tgccctcggg cgcgatgacg tcgatgcagc ggaacacgcc
     1921 cacgttgttg ggcacgtcct ggcgcagcac cgagcggacc gagagatagg tgcacgactt
     1981 cgtgaaggac agcgtcgagt tgagcgcggc cttcacctgg ggcgagctcc ccgtccagtc
     2041 gcacgtgaga tgcccgtcgg ggtggacggt gatggccacc cggagcggga ccggctcgtc
     2101 gctgagcccg tcgctgtcga ggtgatcggt gaacgtgtag acgccctgcg gccagcgggc
     2161 gatctcggcg cgggtgaggc gctccgcgta gtcgagcagc tccgcggtgt agcggcgaac
     2221 ctcgtcgcgt ccgtagcggg tatagagccg ccccagctcg cgctcgccca ccctgcaggc
     2281 cgcgtactgg gcgccgagat cgccgaggac gcgatcgggc acccgcacgt tcttctcgat
     2341 gatgcgccgg accgagcggc tcatcaggcc gctctcgtag agcttgatgg gcggcaggcg
     2401 cagcccctcc tggtagatct cggtggagtc gctcgcgttg gagccgggga cgcgcccgcc
     2461 catgtcggtc tggtggccga tcaccaccgc gaaggcctcg acctccccgt cccagaagat
     2521 cggcatgaag aggaagacgt cggggatgtg catgccgccc tggtagggat cgttcatggc
     2581 caccacgtcg cccggccgga gatcgccggt gaactcggcg agcacgctgg ccatcgcatc
     2641 cggaatggcg ccgaggtgct gggcgatggt cttggcctga gcgatcatgc ggccccgcgc
     2701 gtcgcagagg gcggccgagt agtccatcac gtccttgatg atctcggagc gcgcggtgcg
     2761 gatgaccgtg taggccatct cgtccacgat cgagtcgagc gcgctcttga tgacggccag
     2821 cgtgatggga tcgatcatgc gttctctccg aaggtgatca gcagattgtc caccgcgtcg
     2881 cggcggacgg cccagccggg cggcaccacc accgtcgagt cgtagctctc gacaatgagc
     2941 ggtcccggct ggcccgacgc ggtcaccgcc tcgcgcagcc gcaccggcgt ctccacggcg
     3001 cgcacgcgcc ggtcgaagga cacgagccga tgggcggcgg gcgcgggcgt cccggccgcc
     3061 acgcgcaccg cccggaagtc gaggcggtgg ggacgcaccc cggtcgccac cacgcggacg
     3121 ttgaccagct cgagctcctc gcggctggcg tcgccgtagg tcgccgcgta ctcgcgcgcg
     3181 aaggcctcgc gcagggcggg caggcggtcc gccgcgaggc ggccgcccgg gatcgggatc
     3241 acgagctccg aggcctggcc gacgtagcgg agatcggcct gggcgtcgag acgcacctgc
     3301 tcgcgcggat agccctcctg ggcgagggtg acgagcgcct cgtccgtcat ctcgtcgagc
     3361 cgggcgttcg cccgctcgag gacgcactcg tcgagcggcc cgcccgccgc gcgcacgaag
     3421 tggtgctcca cgtcgctcgc cagcatgccg accgcggtga acacgcccgg ggagaccggc
     3481 acgagcacgc ggcggatgtc gagcgcgcgg gccacgtcgc ccgcgtgcac cgggccgctg
     3541 cccccgaagg ccaccagggt gaagtcgcgc ggatcgcgcc cccgctcgac ggtgaccgag
     3601 cggatcgcgc gcgccatgtt cgcgttggcg acttctctta ttccatgggc ggcttcctcg
     3661 accgagaggc cgaggggctc ggccacgtgg gcccggatcg ccgcgcgcgc ccgcggcgcg
     3721 tcgatccgga gctcgccgcc cgccaggtag gccgggttga gatagccgag ggccacgttg
     3781 gcgtcggtga cggtgggccg ggtgccaccc agggcgtagc acgcggggcc gggatgcgcg
     3841 cccgccgact ccggccccac ccgcaggagc ccgccgtcgt cgatgtgcgc gatggagccg
     3901 gcgcccgcgc ccacctcggc gatgtcgatg gcggggacct tcagcatgta gcccccggcc
     3961 tttatgaagc ggctcgacgt gctgataccc tcgcggaact cgtactcggt ggtgagcgag
     4021 acccgcccgt cctccacgag cgaggccttg gcggtggtgc cgcccatgtc gaaggcgatg
     4081 aggttcggcg tgtcggcgac ctgcccgagc cgcgccgcgc ccgccacgcc cgccgccggc
     4141 cccgacccca ccgcgaacac cggccgctca cccgcgccct gggcgcccac cacgccgccg
     4201 ttggaggcca ccaccaggag cggcgcctcc accccgatcc cgcgcaggcc cgccgcgagc
     4261 gtcgccaggt agcggcgcat cacgggcagg agataggcgt tcaccaccgt ggtgctggtg
     4321 cgctcgtact ccttgatctc gggcagcacg cgatacgacg cggtcacgtc gagggcgggg
     4381 aagcgcgcgg cgagcaggct ctcggccagg cgctcgtggg ccggattcac gtagctgttg
     4441 atgaagctca ccgccaccac gtcggcgccg tcggccacca gccgccccgc ggcggccagc
     4501 aacccctcgc gatcgagcgg gcgcacgacc gcgccgtcgg cgccgatccg ctcgtcgact
     4561 tcgagacgat gccggcgctc gactaggggc tccggcttct cccacgtgag gtcgaagaga
     4621 tcgggggtgc gcacgcgccc gatctcgagc acgtcccgga agccgcgggt ggtcaggagc
     4681 ccgacgcgcg cgcccttctt ctggaggatg gtgttggacg ccaccgtggt gccgtgcacg
     4741 acctcgacaa cggatgcggg ggggacggcg gcggcgcgca ggagcgcgtc gagcccgcgc
     4801 accaccgcga gcccgggatc ctcgggcgtg gaggagacct tgctgacccg cagcgtgccg
     4861 tcgtcgaggc gcagcacgat gtcggtgaag gtgccgccga tgtccacgcc gacgcgcgcg
     4921 ctcacgcgtc cgaccgcagg gtgccggtga gcacggtgac agcggacccg cccacgcgga
     4981 cgcgcgcggc gcgcccggcc ttcgcggtga gctccacctg caggcgcccg gggcggccca
     5041 gcccgtcacc ctgctcggcg gtgaagacga tgcgcccgtc ggcggcgggc agcacgcccg
     5101 cctcgagcag ccagaccccg agcgacgagt ggacggagcc ggtgacgatg tcctccggga
     5161 tgccgtagtg cggagcgaag aagcggcagt ggctgcgcga gccgggatcc accgtctcgc
     5221 gcgagaccac gcagaccccg cgcgcgtgct ggggggcgcg caccccggct aggcgtcgca
     5281 tgtcgggctc gagtgcccgc agcgcggcca gatcgcgcac gggcaggagg agatcctggt
     5341 cgggcgtcac cgcggggcgc gcccagccgc ccaccgcggc ccgggacaat ccgagcgagt
     5401 ccaggatctc ccccaggtcg ccttcgaagg gtcggcacgc gggcagcggc ggctcgagcc
     5461 acatcagcgc gcggcccccg ccgcgctcca cgtccacgcg caggagcccc gacggcgtgt
     5521 cgtaggtgac gcgatcgccc gggaggcggc cgctctcgac cagcgcgtgc gtgccgcaga
     5581 gggcggtgtg cccggagtag ccgacctcgc gcttcggggt gaacatgcgg agccgccagt
     5641 ccacgccggc ctgcgtcgag gcggagacga acgcggtgcc gggcacgagc atctcggcgg
     5701 cgatccgctg catcgccggc gcgtcgaggc gatcggcgtc cagcaccgcc gcggccggat
     5761 tgccggcgaa gggccggtcc gtgaagatgt cggcctggat gatcgcgatg ggcccgctca
     5821 cagccggatg cgctcgcgga ggcgtcgggc ctggcggccg agggcgcggg tggaccacac
     5881 ggtgccgagc gaatgggcca tcaggccgta gacgcgcacg cgcgggacct tcagcgggat
     5941 cgcggggtcg agccagttgg gcgaggcggc gagcaggtcg agggtgcgaa gcccgatcag
     6001 gagcggccac gcgcaggcca ggcgcatccg cgcctcgcgc cgcgggatcg cgaaggtgta
     6061 ctcccagccg gcctcgtagt gatcgagcgc ggtgttgagc cactcgccca ggagcgggcg
     6121 cgccgcgggc gcgcgcgccg gatcgagcag gtcgcgcggc tcgaggcccc accgggccag
     6181 gtcctggcgg ggcaggtagc agcggccctg gcgcaggtcg cgcggcacgt cgcgcaggac
     6241 gttggtaagc tggagtccct tgccgaagcg tgtgcccagc gcgcgcatgc gcggcagatc
     6301 ccaccgcgcg aggcgcgggc gatgggccac gtggacctcg gtccagaact cccccacgca
     6361 gcccgccacc aggtaggtgt agcggtcgag gtcctcgcgg gtctcgagcg cggccagctt
     6421 gccctcgtcc tccccgggga acacgcgcag gtcctggatc tgcccctcga tgatcgtggt
     6481 gagcaccgcg tgcacccgcg cgcggtcggc gggcggcagc gcgcggtaag cggcgaggca
     6541 atcgggcagc cgctcgagca gcgtgcgctc cgcggggagc gcctgcgcgc tggcggtggc
     6601 cccgacggct atcgaacgcc cgtcgtccgc gagcgcggca cgcagggctt ccaggtgggc
     6661 gatgcgcgac atccggtcga tcagccgcgt gtccgcgatc gtgtcggagg cgcgcgcgaa
     6721 caggtaggcg aggccgatgg tgggccgcac cgaccggggc aggacggcca gcgagagata
     6781 gaaggatcga ctgacccgcc ggaggagagc ggcggtgaga tcgcttccgg gcacggcggc
     6841 tagtcg
//
LOCUS       DASUFA010000377        10523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_774379, whole genome shotgun sequence.
ACCESSION   DASUFA010000377 DASUFA010000000
VERSION     DASUFA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10523
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774379"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFX87_13760"
     CDS             complement(<1..239)
                     /locus_tag="VFX87_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4996043.1"
                     /translation="MAEPSVRYTARDGVATITLNRPHVLNALDLALSAALADAAEAAA
                     RDAGVWVVVVRGAGRAFCSGMDRTALASGGIGESF"
     gene            complement(242..1390)
                     /locus_tag="VFX87_13765"
     CDS             complement(242..1390)
                     /locus_tag="VFX87_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996044.1"
                     /translation="MNFALTPEQQEVRALARDFAEREVAPVIRHFDETQEFPHEIMAK
                     LAGTGLLGALVPEEYGGAGLDYVSYALAVEELNRVDASVGITMWAHNSLCTNHIALFG
                     SAAQKAAYLPRLARGAALGAWGLTEPGSGSDAAAMRSRAVPEGDHFVLNGSKAFITNA
                     GVAEITVLMAVTDPARGTKGISAFILERGAPGFTVGKPYRKLGLHASNTAELHLDNVR
                     VPATALCGQRDLGFVNTMQVLEGGRIAMAAMAVGIAQGALDEALKYMKQRSAFGKTLA
                     EFNGLQGMIADIGTEVEAARLLTLRAAVLKDRGRPAKVAASMAKVFASEVAMKAATKA
                     LQIHGGAGYITEFPIERIFRDAKLTEIGEGTSEVQRMVIARDILGVRS"
     gene            complement(1422..1826)
                     /locus_tag="VFX87_13770"
     CDS             complement(1422..1826)
                     /locus_tag="VFX87_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX4996045.1"
                     /translation="MAERKVRVLVAKPGLDGHDRGAKIVARALRDAGFEVIYTGLHQT
                     PEQIVATAVQEDVDAVGLSVLSGAHNYLFGRVLDLLKEKGADDIAVFGGGIIPQDDIQ
                     ALKALGVKELFTPGTSTQDIIRFVRENIRAVA"
     gene            complement(1829..2242)
                     /locus_tag="VFX87_13775"
     CDS             complement(1829..2242)
                     /locus_tag="VFX87_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX4996046.1"
                     /translation="MTDCVFCKIRDGQIPSMKIFEDDKTLVFMDINPLNSGHCLVITK
                     AHAANLFEAAVEDLQAAIATAQRVALAIREALKPDGLNMVQANGAAAFQSVLHYHLHL
                     IPRWANDGKGFDWRLVPGNREQIMKAGERLRALLH"
     gene            complement(2259..3326)
                     /locus_tag="VFX87_13780"
     CDS             complement(2259..3326)
                     /locus_tag="VFX87_13780"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX4996047.1"
                     /translation="MTEPIRINRSTTRKPKPKDADLGFGQIFTDHMFLADFQEEKGWY
                     DPRVEPYGPLSLDPATAVLHYAQAIFDGLKAFRGVDGKIRLFRPQKHVERMNNSARRL
                     CIPPLDADMALQSLVRLVDIDRDWVPSTVGTSLYIRPTIIASEAFLGVRPAKSYIYFV
                     ILSPVGAYYPEGMAPVKILVVDKYVRAVDGGVGGAKTSGNYAASLFASDEAKHAGFTQ
                     VLWLDGVHRKYLDEVGTMNIMVKIGDEIVTPPLTGTILPGVTRDSSLALMRKWGLRVS
                     ERQVSIDEVVEAHERGKLDEVWGTGTAAVISPVGELAYKGRKMVINGGKIGPLTQRLY
                     DAIVAIQYGQAPDPDGWTLTI"
     gene            complement(3339..5006)
                     /locus_tag="VFX87_13785"
     CDS             complement(3339..5006)
                     /locus_tag="VFX87_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX4996048.1"
                     /translation="MDARHGADGKERWEEESYRPFVERTPERDVPYETLSGIPVQPLY
                     TPEDLAGWRYEDKLGYPGEFPYTRGPYPSMYRGRLWTMRMFAGFGRPEDTNARFKYLL
                     EQGQTGLSTAFDMPALMGYDADHPRARGEVGREGVSISTLADFEILFRDIPLDKVTTS
                     MTINCTASVALAMYLALADKQGVGWDKLGGTMQNDMLKEFIAQKEWISPPESAVRVVV
                     DTIEFCSKHVPRFNPVSISGYHIREAGSTAVQELAFTLADGFGYVQAGVERGLDVDDF
                     APRLSFFFDIHNDFFEEIAKLRAARRIWATVMRDRFRARKPESMRLRTHTQTAGVSAT
                     AQQPLNNVARVALQALAAVLGGAQSLHTNSYDEVLALPTEEAVTVALRTQQIIAEETG
                     VALTADPLGGSYFLERLTDQMEVQAMDYIRKIDELGGIVRAIDIGYPQQEIADAAYHY
                     QLMDDRGQKAVVGVNKYVMPDEKPVEYLRIDPAIEQEQVERVGRVKASRDAARVEKRL
                     KQLTAACRDGQNVMPVLVDAVKDYVSLGEIADVYRRVFGLYREPIIF"
     gene            complement(5022..6179)
                     /locus_tag="VFX87_13790"
     CDS             complement(5022..6179)
                     /locus_tag="VFX87_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4996049.1"
                     /translation="MDLDLTEEQTMIRGLARDFAEKEVRPIAEAIDREARFPRATVAR
                     MAELGLMGIAVPEAYGGSGGDTVGYALAVEEVARACASHAVIMSVNNSLYCDPVCKHG
                     TEEQKARFLTPFAAGQKLGCFALTEPEAGSDASNQSTLAVRDGDGYVLDGRKIFVTNG
                     REASAALVFAQTDRAARHHGISAFLVEKGTPGFTVVKTEDKLGIRASDTAELLFERCR
                     VPAANRLGEVGQGFRIALGTLDAGRIGIAAQAVGIAGAAYAAAAAYARERKSFGVPIG
                     QHQMVQWMLADMATAIEAARLLTLRAAWRKDSGAPYGPEAAMAKLFAAETAMRVATDA
                     IQVHGGYGFIKEYQVERYFRDAKITQIYEGTSQIQKVVIARHLLAGAARVA"
     gene            complement(6291..7277)
                     /locus_tag="VFX87_13795"
     CDS             complement(6291..7277)
                     /locus_tag="VFX87_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX4996050.1"
                     /translation="MPGAYWSMVEDDRQGAPKKVMAEVLGEASRLAGAAGAQAEAVWV
                     TDKATPEGLKQLGEWGAKRVWLFENAAFAPYRAEVWTPVVAELAGKEAPQAIFAPVTT
                     RQREFMARLAARLGAGLAADSTALTAEGGKLVATRPVYAGKLLAKMTWAKTPWMATLR
                     PNVFRPGDGAGGAATVEKPTASIPAESMKLVERKQEASTGLPELSEADIVVSGGRGLK
                     GPENFVILEELAKVMGAAVGASRAAVDAGWRPHRFQIGQTGRTISPKLYLGFGISGAI
                     QHLAGMRTSKVICAINKDPEAPIFKIADYGIVGDLFEVAPLLAKEFKQLLEK"
     gene            complement(7305..8084)
                     /locus_tag="VFX87_13800"
     CDS             complement(7305..8084)
                     /locus_tag="VFX87_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX4996051.1"
                     /translation="MKILVMIKQVPDTATQVKIGGDGRAIDTTGITWIVSPYDEFAVE
                     EALRIKEKRGQGEVVAVSLGPDRGKEALRSCLAMGADRAIHLNDPAWAEADTLTTARA
                     LAGVIKQEAPALALFGRQAIDDDMGAVAAQVAEVLGWPCASWIMEEAVDEGGKTIRVG
                     RQVEGGLEIFDLPLPAVASAQKGLNEPRYPTLKGIMGAKKKEIKDVKAADLGLAAEPP
                     ALSVVKLESLPPRPPGRIIQGEPAAAAKELVRALREDSKAI"
     gene            complement(8142..9008)
                     /locus_tag="VFX87_13805"
     CDS             complement(8142..9008)
                     /locus_tag="VFX87_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4996052.1"
                     /translation="MGAHLASRVPRYHQIAQTLRERIATSGQGPGERLDNQRSLAREF
                     GVTLMTLRQALDLLERDGLIARRHGLGTFVARPAIDYDILQLRALAGDLSALGEDVAT
                     RFLRSRFEPADRRVAGALGLAEQAEVFVLERLRLVDGEPVSFQASYLPAELGTEVSRA
                     DLAVTPLRQVLTFKLGLEITAARETVSAVPLDRRAARELGCRPGIAAFRSDRVSVGAD
                     GAPIVYDRVFIPGDRFRITRNLKYDTGNVTTATINDASTGPIASREVGRMSAEGAKEP
                     GRQTEIFDVQAR"
     gene            complement(9184..10245)
                     /gene="trpD"
                     /locus_tag="VFX87_13810"
     CDS             complement(9184..10245)
                     /gene="trpD"
                     /locus_tag="VFX87_13810"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX4996053.1"
                     /translation="MITEAVRALVDRRDLTRIEAAAAMEAIMSGAATNAQIAAFLTAL
                     RMKGETVEELIGFAQVMRQKVVKVRASGADLAQTGTDREMLIDTCGTGGDASGTFNVS
                     TATAFVAAGAGVKVAKHGNRSVSSLCGSADVVETLGINIELSPPKVARCVDEVGIGFL
                     YAPLLHTAMKHVMAARREMGVRTVFNMLGPLTNPAGANAQVIGVYAAPLTELLARALA
                     ELGTIRAFVVHGADGLDEISNTGESHIAEVHEGVVRTSRVRPEDFGLPRAAIGDLQGG
                     DRQENADIIRRVLGGEAGPRRDIVLMNAAAALVAGGKARDFKEGVALAAQSIDSGAAA
                     AKLAALVAFSQRLSAEAPI"
     gene            complement(10254..>10523)
                     /locus_tag="VFX87_13815"
     CDS             complement(10254..>10523)
                     /locus_tag="VFX87_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4996054.1"
                     /translation="QEGVDRFGAERFDVVFTDLAMPGMTGWQVARAVKDRAPEVPVVM
                     MSGFGVEVAPEDLSTHGVDLVLAKPLQIQDVLRALTTVRPGTGRP"
BASE COUNT         1444 a         3797 c         3578 g         1704 t
ORIGIN      
        1 tagaagctct cgccgatccc gccggaggcg agcgccgtcc ggtccatgcc cgagcagaac
       61 gcgcggcccg caccccgcac caccacgacc cacacgcccg cgtcgcgggc ggcggcctcc
      121 gcggcgtccg ccagcgccgc ggacagcgcc agatccagcg cgttgagcac gtggggccgg
      181 ttcagcgtga tggtggccac cccgtctctt gccgtgtagc gcacgctcgg ctcggccacg
      241 ctcagctccg cacgcccagg atgtcgcggg cgatgaccat gcgctggacc tcggaggtgc
      301 cttcgccgat ctcggtgagc ttggcgtcgc ggaagatccg ctcgatgggg aactcggtga
      361 tgtagcccgc gccgccgtgg atctggagcg ccttggtcgc cgccttcatc gcgacctcgg
      421 aggcgaacac cttggccatc gaggccgcga ccttggccgg gcggccgcgg tccttgagga
      481 cggcggcccg cagggtcagc agtcgggccg cctccacctc ggtcccgatg tccgcgatca
      541 ttccctgcag gccgttgaac tcggccaggg tcttgccgaa ggccgagcgc tgcttcatgt
      601 acttgagcgc ctcgtcgagc gcgccctggg cgatccccac cgccatggcc gccatcgcga
      661 tgcggccgcc ctcgagcacc tgcatcgtgt tgacgaagcc gaggtcgcgc tggccgcaga
      721 gggcggtggc gggcacccgc acgttgtcga ggtggagctc ggcggtattg gaggcgtgca
      781 ggccgagctt gcggtacggc ttgcccaccg tgaacccggg ggcgccccgc tcgaggatga
      841 aggcggagat gcccttggtg ccgcgcgccg gatcggtcac cgccatcagc accgtgatct
      901 ccgccacccc cgcgttggtg atgaaggcct tgctcccgtt gagcacgaag tgatcgccct
      961 ccgggaccgc gcgcgagcgc atcgcggccg cgtcggagcc ggagccgggc tcggtgagcc
     1021 cccacgcgcc cagcgccgcg ccgcgggcca gccgcggcag gtaggccgcc ttctgcgcgg
     1081 ccgagccgaa gagcgcgatg tggttggtgc agagcgagtt gtgcgcccac atcgtgatgc
     1141 cgacggaggc gtccacccgg ttcagctcct ccaccgcgag ggcgtaggag acgtagtcga
     1201 ggccggcgcc cccgtactcc tcgggcacga gcgcgcccag gagcccggtg cccgcgagct
     1261 tggccatgat ctcgtgcggg aactcctgcg tctcgtcgaa gtggcggatc accggcgcga
     1321 cctcgcgctc cgcgaagtcc cgggcgaggg cccgcacttc ctgctgctcc ggggtgaggg
     1381 cgaagttcat gggctcgcgc tatccgcaaa cagggtcact actacgcaac ggccctgatg
     1441 ttctcccgca cgaagcggat gatgtcctgg gtcgaggtcc ccggcgtgaa cagctccttg
     1501 accccgaggg ccttgagggc ctggatgtcg tcctgcggga tgatgccgcc gccgaacacc
     1561 gcgatgtcgt ccgcgccttt ctccttgagc aggtcgagca cgcgtccgaa gagatagttg
     1621 tgcgcccccg agagcaccga gaggcccacc gcgtccacgt cctcctgcac cgcggtcgcc
     1681 acgatctgct ccggggtctg gtgcagtccc gtgtagatca cctcgaagcc cgcgtcccgg
     1741 agcgctcgcg ccacgatctt cgccccccgg tcgtgcccgt ccagacccgg cttggccacc
     1801 agcacgcgca ccttgcgctc ggccatgatc agtgcaggag cgcccgcagg cgctcccccg
     1861 ctttcatgat ctgctcgcgg ttgcccggca ccagccgcca gtcgaagccc ttgccgtcgt
     1921 tggcccagcg cgggatcagg tgcaggtggt agtgcaggac ggactggaag gccgccgccc
     1981 cgttggcctg caccatgttg agcccgtcgg gcttgagcgc ctcccggatc gccagggcca
     2041 cccgctgggc ggtggcgatg gcggcctgga gatcctcgac cgcggcctcg aagagattgg
     2101 ccgcgtgggc cttcgtgatc accaggcagt ggcccgagtt gagcgggttg atgtccatga
     2161 agaccagcgt cttgtcgtcc tcgaagatct tcatcgaggg gatctggccg tcgcggatct
     2221 tgcagaagac gcagtcggtc atcgtgaccc ctccgtcctc agatcgtgag cgtccagccg
     2281 tcgggatcgg gcgcctgccc gtactggatg gccacgatgg catcgtagag ccgctgggtc
     2341 agcggcccga tcttcccgcc gttgatcacc atcttccggc ccttgtaggc caactcgccc
     2401 accggcgaga tgaccgccgc ggtgccggtg ccccacacct cgtccagctt gccgcgctcg
     2461 tgggcctcca ccacctcgtc gatcgagacc tggcgctcgc tcaccctgag cccccacttg
     2521 cgcatcaggg ccagcgagga atcgcgggtg acgccgggca ggatggtccc ggtcagcggc
     2581 ggggtgacga tctcgtcgcc gatcttcacc atgatgttca tggtgcccac ctcgtcgagg
     2641 tacttccggt ggacgccgtc gagccacagc acctgggtaa atcccgcgtg tttcgcctcg
     2701 tcgctcgcga agaggctcgc cgcgtagttg ccggaggtct tggccccgcc caccccgccg
     2761 tcgaccgcgc ggacgtactt gtccaccacc aggatcttga ccggcgccat gccctccgga
     2821 tagtaggcgc ccaccgggga gaggatgacg aagtagatgt acgacttggc cggccgcacc
     2881 ccgaggaacg cctcgctcgc gatgatggtg ggccggatgt agagcgaggt gcccaccgtg
     2941 ctcggcaccc agtcgcgatc gatgtccacg aggcgcacca gggactgcag cgccatgtcc
     3001 gcgtcgagcg gcgggatgca gaggcgccgc gccgagttgt tcatccgctc cacgtgcttc
     3061 tgcggccgga agagccggat cttgccgtcc acgccgcgga acgccttgag gccgtcgaag
     3121 atggcctggg cgtagtgcag gaccgcggtg gccgggtcga gcgagagtgg cccgtacggc
     3181 tcgacccgtg ggtcgtacca ccccttctct tcctggaagt cggccaggaa catgtgatcg
     3241 gtgaagatct gcccgaatcc caggtcggcg tccttcggct tgggcttgcg ggtggtggac
     3301 cggttgatcc ggatcggctc ggtcatggtt ggctcccctc agaagatgat cggctctcga
     3361 tagaggccga acacccggcg atacacgtcg gcgatctcgc cgagcgagac gtagtccttc
     3421 acggcatcca ccagcaccgg catgacgttc tggccgtcgc ggcaggcggc cgtgagctgc
     3481 ttcaggcgct tctcgacccg cgccgcgtcg cgcgaggcct tgacgcgccc cacccgctcg
     3541 acctgctcct gctcgatcgc ggggtcgatg cggagatact ccaccggctt ctcgtcgggc
     3601 atcacgtact tgttcacgcc caccaccgcc ttctgcccgc ggtcgtccat gagctggtag
     3661 tggtaggccg cgtccgcgat ctcctgctgc gggtagccga tgtcgatggc ccgcacgatg
     3721 ccgcccagct cgtcgatctt gcggatgtag tccatcgcct gcacttccat ctggtcggtc
     3781 agccgctcaa ggaagtagga gccgcccagc ggatccgcgg tgagcgccac cccggtctct
     3841 tccgcgatga tctgctgggt gcggagcgcc accgtcaccg cttcctcggt gggcagggcc
     3901 agcacctcgt cgtaggagtt ggtgtggagc gactgggccc cgcccagcac cgcggccagc
     3961 gcctgcagcg ccacccgcgc cacgttgttg agcggctgct gggcggtggc ggagacgccc
     4021 gcggtctggg tgtgggtgcg cagtcgcatc gactcgggct tccgcgcccg gaagcggtcc
     4081 cgcatcacgg tggcccagat gcgccgggcc gcccgcagct tggcgatctc ctcgaagaag
     4141 tcgttgtgga tatcgaagaa gaacgacagg cgcggcgcga aatcgtccac gtcgagcccg
     4201 cgctcgaccc ccgcctgcac gtagccgaag ccgtcggcca gggtgaaggc cagctcctgc
     4261 accgcggtcg agccggcctc ccggatgtgg tagccggaga tggacaccgg gttgaagcgc
     4321 ggcacgtgct tgctgcagaa ctcgatggtg tccaccacca cccgcaccgc ggactcgggc
     4381 ggcgagatcc actctttctg ggcgatgaac tccttgagca tgtcgttctg catggtgccg
     4441 cccagcttgt cccagcccac cccctgcttg tcggccagcg ccaggtacat ggcgagcgcc
     4501 accgacgcgg tgcagttgat cgtcatggac gtcgtgacct tgtccagcgg gatatcgcgg
     4561 aacaggatct cgaagtcggc gagcgtggag atggacacgc cctcgcggcc aacttcgccg
     4621 cgcgcccggg ggtgatcggc gtcgtagccc atcagcgcgg gcatgtcgaa ggcggtcgag
     4681 agcccggtct gtccctgctc gaggaggtac ttgaagcggg cgttggtgtc ctccggccgg
     4741 ccgaacccgg cgaacatccg catcgtccac aggcgcccgc ggtacatcga cggatacggc
     4801 ccgcgcgtgt acgggaactc gcccgggtaa cccagcttgt cctcgtagcg ccagcccgcg
     4861 agatcctccg gggtgtagag cggctgcacc gggatcccgg agagcgtctc gtagggcacg
     4921 tcgcgctcgg gggtgcgctc gacgaacggc cggtagctct cctcttccca ccgctccttg
     4981 ccgtcggccc catgccgagc atccatgatc gcgagtcctc ctcaggctac ccgggcggcg
     5041 ccggcgagca ggtgacgggc gatgaccacc ttctggatct gcgacgtgcc ctcgtagatc
     5101 tgggtgatct tggcgtcgcg gaagtaccgc tcgacctggt actccttgat gaagccgtaa
     5161 ccgccgtgca cctggatcgc gtcggtggcg acgcgcatcg cggtctccgc cgcgaagagc
     5221 ttggccatgg ccgcctccgg cccgtacggc gcgccggaat ccttgcgcca ggccgcgcgc
     5281 agtgtgagca ggcgcgccgc ctcgatcgcg gtcgccatgt cggccagcat ccactgcacc
     5341 atctggtgct ggccgatggg cacgccgaag ctcttgcgct cgcgcgcgta ggccgccgcc
     5401 gccgcgtagg cggcccccgc gatgcccacc gcctgcgcgg cgatgccgat ccgcccggcg
     5461 tccagcgtcc cgagcgcgat cctgaacccc tgccccacct cgccgagccg gttggccgcg
     5521 ggcactcggc agcgctcgaa gaggagctcg gcggtgtccg aggcgcggat gccgagcttg
     5581 tcctcggtct tgaccaccgt gaagcccggc gtgcccttct cgaccaggaa agcgctgatg
     5641 ccgtgatgcc gggccgcgcg atcggtctgc gcgaagacga gggcggccga agcctcgcga
     5701 ccgttggtca cgaagatctt ccgcccgtcg agcacgtagc cgtcgccgtc ccgcaccgcg
     5761 agcgtgctct ggttgctcgc gtccgagccc gcctccggct cggtgagcgc gaaacagccg
     5821 agcttctggc cggccgcgaa tggggtcagg aagcgggcct tctgctcctc ggtgccgtgc
     5881 ttgcacaccg ggtcgcagta gagcgagttg ttcaccgaca tgatgaccgc gtgggaggcg
     5941 caggcccggg ccacctcctc gacggccagg gcgtagccca ccgtgtcgcc gccgctgccc
     6001 ccgtacgcct ccggaaccgc gatgcccatc aggcccagct cggccatgcg ggccacggtg
     6061 gcccggggga agcgcgcctc gcggtcgatg gcctccgcga tcgggcgcac ctctttctcc
     6121 gcgaaatcgc gggcgaggcc ccggatcatc gtctgctcct ccgtgaggtc taggtccact
     6181 cctgctttcc tttcgtctac ttcttcaagc tcttcagctg cgggggccca gacacggccc
     6241 ccgcactccc ccagatggcg gacactcgtt tacggcgtcc ctgttcgcgt ttacttctcc
     6301 aggagctgtt tgaactcctt ggccagcagg ggggcgacct cgaagaggtc gccgacgatg
     6361 ccgtagtcgg cgatcttgaa gatcggggcc tcgggatcct tgttgatcgc gcagatcacc
     6421 ttcgacgtgc gcatgccggc caggtgctgg atcgcgcccg agatgccgaa gcccaggtag
     6481 agcttgggcg agatcgtgcg gccggtctgg ccgatctgga agcgatgcgg gcgccagccc
     6541 gcgtccaccg cggcccgaga ggcgccgacc gccgccccca tcaccttggc gagctcttcc
     6601 aggatgacga agttctccgg ccccttgagg ccgcggccgc ccgagacgac gatatccgcc
     6661 tcgctcagct cgggcaggcc ggtggaggcc tcctgcttgc gctcgaccag cttcatgctc
     6721 tcggccggga tgctcgcggt gggcttctcc accgtcgcgg cgccgcccgc gccgtcgccc
     6781 gggcggaaca cgttgggccg cagcgtcgcc atccacggcg tcttcgccca cgtcatcttc
     6841 gcgaggagct tgcccgcgta gaccggccgg gtcgccacca gcttgccgcc ctcggcggtg
     6901 agcgcggtgg agtccgcggc gagccccgcc cccaggcgcg cggccagccg ggccatgaac
     6961 tcgcgctgcc gcgtggtcac cggggcgaag atggcctgcg gcgcctcctt gccggccagc
     7021 tccgccacca ccggcgtcca gacctccgcc cggtacggcg cgaatgccgc gttctcgaac
     7081 agccagacgc gcttggcgcc ccactccccg agctgcttca gcccctccgg cgtggccttg
     7141 tccgtcaccc agaccgcctc ggcctgcgcc cccgccgcgc cggccaggcg cgaggcctcg
     7201 cccagcacct cggccatgac cttcttgggt gcgccctgcc gatcgtcttc caccatgctc
     7261 cagtacgcgc ctggcatctc gatccccttt atcgttgtgc gacgttagat ggctttcgag
     7321 tcttctcgaa gggcgcggac gagctccttg gcggcggccg cgggctcgcc ctggatgatg
     7381 cggccgggcg ggcgcggcgg caggctctcg agcttgacga cgctcagcgc gggcggctcg
     7441 gccgcgaggc cgagatcggc cgccttcacg tccttgatct ccttcttctt ggcgcccatg
     7501 atccccttca gcgtgggata gcgcggctcg ttgagcccct tctgcgcgga ggccaccgcg
     7561 ggcagcggta ggtcgaagat ctcgagcccg ccctccacct ggcgccctac ccggatcgtc
     7621 ttgccgcctt cgtccaccgc ctcctccatg atccaggagg cgcacggcca gcccagcacc
     7681 tcggcgacct gcgcggcgac cgcgcccatg tcgtcgtcga tggcctgccg gccgaagagg
     7741 gccagcgcgg gcgcttcttg cttgatcacg ccggcgagcg cgcgcgcggt cgtcagcgtg
     7801 tcggcctccg cccaggcggg atcgttcagg tggatggcgc gatcggcgcc catggccagg
     7861 cacgagcgca gcgcctcctt gccgcggtcg gggccgagcg agacggcgac cacctcgccc
     7921 tgcccgcgct tctccttgat ccggagcgcc tcctccaccg cgaactcgtc gtacggggag
     7981 acgatccagg tgatgcccgt ggtgtcgatg gcgcgcccgt caccgccgat cttcacctgc
     8041 gtggccgtgt ccgggacctg cttgatcatg acgaggatct tcatgcgcgg cctttcatag
     8101 cggttgaacg tggaggattt cggtttggcg gctgggtctt gtcatcgcgc ttgaacgtcg
     8161 aagatttcgg tttggcggcc gggctccttc gcgccttcgg cgctcatccg acctacttca
     8221 cgtgaggcga ttggacccgt cgacgcgtcg ttgatggtgg cggtggtcac gttcccggtg
     8281 tcgtatttca ggttgcgggt gatgcggaaa cggtcgcccg ggatgaagac gcggtcgtag
     8341 acgatcgggg cgccgtccgc gcccaccgag acgcggtcgg agcggaaggc ggcgatgccg
     8401 ggacggcagc ccagctcgcg ggcggcgcgg cgatcgaggg gcaccgcgga gacggtctcc
     8461 cgggcggccg tgatctcgag gcccagcttg aaggtcagca cctggcggag cggcgtcacc
     8521 gccaggtcgg cgcgcgagac ctcggtgccc agctcggcgg gcaggtacga ggcctggaag
     8581 ctcaccggct cgccgtccac gagccgcagc cgctcgagca cgaagacctc cgcctgctcc
     8641 gccagtccga gcgccccggc gacccgccga tcggcaggct cgaagcggct ccggaggaac
     8701 cgcgtcgcga cgtcctcgcc cagcgccgag aggtccccgg cgagcgcgcg aagctgcagg
     8761 atgtcgtagt cgatggcggg ccgcgcaacg aaggtcccga ggccgtgccg gcgggcgatg
     8821 aggccgtcgc gctcgagcag atcgagcgcc tggcgaaggg tcatgagcgt gacaccgaac
     8881 tcgcgcgcga gactgcgctg attgtccagg cgctcgcccg gcccctggcc tgacgtggcg
     8941 atccgctcgc gaagcgtctg agcgatctgg tgataccgcg gcacgcgact cgccaagtga
     9001 gcccccacgc tcagtgttct agaactcctg cggccgctca acttccttgt cgagcctcat
     9061 cgggcttgtc gagcatcgga tcgggattca ctcaggcgcg acagggactt actgcgaccg
     9121 gcgcccattg taaccggggc gggctcacgg ggtcaatggc cccggcggcc gcgcccggtg
     9181 ggactagatg ggggcctcgg cgctcaggcg ctgggagaac gccaccagcg cggcgagctt
     9241 tgccgcggcg gcacccgagt cgatcgactg ggcggccagc gccacgccct ccttgaagtc
     9301 gcgcgccttg ccgcccgcca ccagcgccgc cgccgcgttc atcagcacga tgtcgcggcg
     9361 cggccccgcc tcgcccccga gcacgcgccg gatgatgtcc gcgttctcct gccggtcgcc
     9421 cccctgcagg tcgccgatgg cggcccgcgg caggccgaag tcctccgggc gcacgcggct
     9481 ggtccgcacc acgccctcgt gcacctcggc gatgtggctc tcgccggtgt tcgagatctc
     9541 gtcgagccca tcggcaccgt ggacgacgaa ggcgcggatc gtccccagct cggccagcgc
     9601 gcgggcgagc agctcggtga gcggggccgc gtacaccccg atcacctggg cgttggcccc
     9661 cgcgggattg gtgagcggcc cgagcatgtt gaagacggtg cgcacgccca tctcgcggcg
     9721 tgccgccatc acgtgcttca tggcggtgtg cagcagcggc gcgtagagga agccgattcc
     9781 cacctcgtcc acgcagcgcg ccaccttcgg cgggctgagc tcgatgttga tcccgagcgt
     9841 ctccaccacg tcggcggacc cgcagagcga ggagacggag cggttgccgt gcttggccac
     9901 cttgaccccc gcgcccgccg ccacgaacgc ggtcgcggtc gagacgttga aggtgccgga
     9961 cgcgtcgccc ccggtgccgc aggtgtcgat cagcatctcg cggtcggtgc ccgtctgagc
    10021 cagatccgcg ccgctggcgc ggactttgac caccttctgc cgcatcacct gggcgaagcc
    10081 gatcagctcc tcgaccgtct cgcccttcat gcgcagcgcg gtgaggaacg cggcgatctg
    10141 ggcattggtg gccgcgcccg acatgatggc ttccatcgcg gccgccgcct cgatccgggt
    10201 gaggtcgcgc cggtccacga gcgcgcgcac cgcctcggtg atgatggggc tggtcacggc
    10261 cggcccgtgc ccgggcggac ggtggtcagc gcgcgcagga cgtcctggat ctgcagcggc
    10321 ttggccagga ccaggtccac gccgtgcgtg gagagatcct ccggcgccac ttccaccccg
    10381 aacccgctca tcatcaccac cggcacctcc ggcgcccggt ccttgaccgc gcgcgcgacc
    10441 tgccagcccg tcatgccggg catcgccagg tccgtgaaga ccacgtcgaa tcgctccgcg
    10501 ccgaaccggt ccacgccctc ctg
//
LOCUS       DASUFA010000378        14284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_774401, whole genome shotgun sequence.
ACCESSION   DASUFA010000378 DASUFA010000000
VERSION     DASUFA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14284
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774401"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VFX87_13820"
     CDS             complement(<1..1307)
                     /locus_tag="VFX87_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4996055.1"
                     /translation="MAALIRGGQVLTGTPAALRRADILIDGDRIAALGPGLAAPAGAR
                     VIEAADRIVLPGMGNAHTHAASHLARGRVGSWTLEDLLTYSPANSGFRTPDDEYLSAA
                     IGAIEMLKTGCTGAYDLYMAVPAITDETFAAVVQAYTDVGVRVVLAPAVADVVFYQTV
                     PGLTDLLPPDLRRTVQDIRPSPTKGLLDLTERAIRRWHGSAEGRVRVAVAPTIPNQAT
                     DELLDGCAALSREHGVGIHTHLAESKVQAVESRRRWGKSIVARLADHRVLGPGFVGAH
                     SIWLDDDDLGMLADAGAAVAHNPGSNLRLGVGIAPVREMLDRGLAVGLGTDGSTCADN
                     QNLFEALRIASVISTLRFPHETARWLDADTVWGLATSGTARVLGQSEDLGALAPGRKA
                     DLVLLRTDSVFLRPLADPVKALVYAETGASVDTVLVDGRVVLE"
     gene            complement(1318..1977)
                     /locus_tag="VFX87_13825"
     CDS             complement(1318..1977)
                     /locus_tag="VFX87_13825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4996056.1"
                     /translation="MQPGGHPASARRERDLIFLIRHGETLGNASRTVQLPDNPLSPRG
                     IAQAERLARRLEREGIGAILSSDYSRAAQTAERLARVTGLPVRHDPLLQERNFGDIRG
                     TPYAKLAFDMFALDYAPPNGETWDVFHARVDQAWAIVQEQATTTSGHLAVVTHGLVCR
                     SLAARHLILPEGEDVPERWENTALTIIDSPAPWRVRLLNCVAHLDDDAATQPLSSSGA
                     V"
     gene            complement(1929..3125)
                     /locus_tag="VFX87_13830"
     CDS             complement(1929..3125)
                     /locus_tag="VFX87_13830"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4996057.1"
                     /translation="MADRNPRGPLDGVTVLDMTEHMAGPFCTMILADMGADVIKVERP
                     GAGDSSRGMGDGSERNPYFRYINRNKRSLTLDYKGEPGREIFLKLIPSMDVLVENYRP
                     TVMDRAGLGWETLRQVNPRLIYAQLSGFGSDGPYREKGGFDLIAQGMGGIMHVTGEPD
                     GPPTSVGLPICDLGTGMWGAQGVLAALYERQRTGLGQKVECSLLETAVGFSSWTSAGW
                     LADHAEPVRMGSRHRQNAPYQRFETKDGYMMIGAAGQSIWQRCARALGHPEWLEDPRF
                     ARSPERRKNRFALEKEITAVLATAPTSHWTKVLDDAGVPCGPVYNYEQLFSDPQVQHR
                     EMVVHANDAELGRVPHIRTPIRLSASDVAVRRTAPKLGQHTAEILAGLGYSPADIQRL
                     RGESVI"
     gene            complement(3148..3672)
                     /locus_tag="VFX87_13835"
     CDS             complement(3148..3672)
                     /locus_tag="VFX87_13835"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4996058.1"
                     /translation="MADYHEIPDGPAPTKRELLAALAASRDEVLALVRAMEPARLEEG
                     RYENGWNGRQILAHIASIEWSYPRLIDIARTPPAPVEQAPPTRAMKGGNDAYNDRQVA
                     KREGMTVADLLAEFETNRAATIQAVEAADEELFARRIRSAGGVVGPLATVFHQIAVAH
                     VLGHARDLTGDYTR"
     gene            complement(3705..4595)
                     /locus_tag="VFX87_13840"
     CDS             complement(3705..4595)
                     /locus_tag="VFX87_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4996059.1"
                     /translation="MSSSARRQGLLALLIVIVAWGLTWPVNKVVLATLSPLWAVTLRS
                     AIATVALFALTAWRGRVTLPPRQDLPVLLSITLLHMVGFNLLASWGLGLVPTGRTVVL
                     AYTTPLWVTPGAALFLREPLTARRGAGVLIGLAGLVTLFNPLALDWGDRDVVLGNLAI
                     LAGALLWALSILHIRAHRWVSTPFALIPWETLLATALLVPFALATTAPAPARWTPSFV
                     TLLLYLGIVGTAIAYWATATASRHLPAVTTSLGLLATPVVSVITATVWLGEPLTLSLV
                     IAIVLVLGGVAIGSSGRAAT"
     gene            4723..5823
                     /locus_tag="VFX87_13845"
     CDS             4723..5823
                     /locus_tag="VFX87_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5134..5136,aa:Sec)
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
                     /protein_id="HEX4996060.1"
                     /translation="MVTLLTESGEHEVTGAEPSTEALWCPANEALQATGWAAKPEGLC
                     QGEVCVPLPAGREREFVDGGRINLAALWRRLGRPAVRSERGHVWVLSESARERKAALA
                     SLEAPDFALPDPSGRMHRLSDYRGKKVLLVTWASWUGCRRDLPGWQALQEELAGDNFM
                     VVAVAMDSRDGDPLPWIEAAKPTYPTLIDREHRLAEFFGIVNVPQAVWIDEAGRIVRP
                     AEPAGAYEGFRQMNRATREMPEEVARRTAQAKALYLDAIRDWARNGERSAYAFTAEQA
                     RAHLPAVTEDIATAQALFRLAQLLIRRGERGEAERWIREASRLHPDSWCIWRQGAGVT
                     ELGLAAQPDFWERVDGLGAKRYYAPVDMPGMP"
     gene            5831..6886
                     /locus_tag="VFX87_13850"
     CDS             5831..6886
                     /locus_tag="VFX87_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996061.1"
                     /translation="MKSPRFGLNRFDSRSVDAFAVDVRRAEQLGWDAALQPDSQLRRR
                     DTYVLLAAAARATERITLGPLLANPVNRHPTVTASSIATIDELAPGRVLLGWGVGDTA
                     VRLAGLRPARVAELEAGTRLMRALLAGEAVQVGAARPARLPHHRPVPVWVAAGGPKTL
                     RMAGGVADGVFIRVGTHAANIATAVRAIRAGAAEAGRDPSVVKLGAIFHTVLVDEPSR
                     ALTMARSMAAGYYEYSPALFDPPALEWTGPDPETLKRDRGVWPDFHHAPDLEASGRVV
                     DFLPVEAADAFSLRGGTGEVAERLLAALRAAPATFDHVVLHPIPDPKWPGDPENDYTA
                     RIAREVLPRVRKELETR"
     gene            complement(6883..7743)
                     /locus_tag="VFX87_13855"
     CDS             complement(6883..7743)
                     /locus_tag="VFX87_13855"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4996062.1"
                     /translation="MATMGHEIDVKDVEYLRHGDRPLLARMFVPRGDGPFPMIVELHG
                     GAWSRGDRTNDTIMNEPLARSGVVVAALDFRQPPEASYPGSLVDISYAIRWLKSRAAE
                     FHGRPDQVGLMGASSGAHQAMLLAMKPRDPKYAALPLPAGAPTVDGSVRCAVLCWPVI
                     DPLSRYRYAKALKAGGAPYPDVVDRVLPGHDAYWRTEEAMAEGNPTLALERGERVETP
                     PVVYIQGSRDMAHPRPDLDRFVAQYRKIGGQVDLELIEGEAEGYISKKPAAAERTISR
                     IIEFVHKQLG"
     gene            7886..8167
                     /locus_tag="VFX87_13860"
     CDS             7886..8167
                     /locus_tag="VFX87_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4996063.1"
                     /translation="MKACADGPSGLARLRAFKPDVALIDLGLPGMDGYAVAREARGRP
                     ETRAIRLVAVTGYGQAEDRRRALAAGFDLHVTKPVDASMLDEVLGPATW"
     gene            complement(8157..9971)
                     /locus_tag="VFX87_13865"
     CDS             complement(8157..9971)
                     /locus_tag="VFX87_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4996064.1"
                     /translation="MAESARSVKPLVVMAALVVGVAALYLARGVLIPIALAILLTFLV
                     HPVVAPLARLGLGRALSVGIVVTMLFATLGVVAWGLAAELTLLSTGIPGYRDNLIAKI
                     AYVRRMGRGGVLEKAQSAVTEVTKELQKDPTTPAPPRGTPPPVVVRPQPTGLWQLPTL
                     IEGLGGAATVLVLVIFMLIEQAELRNRVLRLVGYRKLATTTRALDEAAQRISRYLLMQ
                     SIINASFGLGIAVGLLLLGVPYALLWGFLAAVLRFIPYVGAWLAALILIVFALAAFPT
                     WHQPLLVAGLFVLLELLCSMALEPLLYGQSAGVSQVALLCSVAFWAWLWGPVGLLLAT
                     PLTVCLVVFAKHVPGMEFIGILMADVPSIAPPVAYYQRLLAEDESEAARIAAEYAKDH
                     PLEAVYDDLILPALGRARRDRRHGQLPEADERYVWRATRDIVLGLQAKRETEQRAADA
                     EASAAPPEASPPTRKIHLLASPVRDEADQLGLIMLGHLIDPACWTIELTSPQLLASEV
                     IALVESSGPAVVCLGALPASGLAARTRYLSKRLRARFPELRIIVGRWGLREEDGTARR
                     HLEAAGASYVGTTLVETREHLQTVYGLEPVVTRAAITT"
     gene            complement(10123..10584)
                     /gene="msrB"
                     /locus_tag="VFX87_13870"
     CDS             complement(10123..10584)
                     /gene="msrB"
                     /locus_tag="VFX87_13870"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX4996065.1"
                     /translation="MATTKRPTDADLKSRLTPEQYNVTQCSATEPPFRNEFWNHHEAG
                     IYVDVVTGEPLFSSLHKFDSGTGWPSFDRPLEAETVKEHADNSYGMRRVEVRSAEGDS
                     HLGHLFPDGPPSTGLRYCINSAALRFIPVAKLEAEGYGKYLPLFEKEKPGP"
     gene            10618..11508
                     /locus_tag="VFX87_13875"
     CDS             10618..11508
                     /locus_tag="VFX87_13875"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996066.1"
                     /translation="MSDEISIQFTRFSAFYSPLIATIAGGFLQQEGFAPKHSIAPAGK
                     SAIEGVVAGTVHVCQSAPSQGFGPLEKGQAPPVVHFAQINEMDGFFLTGRAPDPAFTW
                     DRLKGRRVLVDHGGQPLAMFKYACHKRGLDFASIVAVNVPSDQMDAAFRKGEGDYIHQ
                     QGPAPQQLEHDHAGHVVASVGQAIGPVAFSSLAATRAWLATDMARRFMRAYRKARAWL
                     LATPAAEVAKAEASFFPEIDQAVLAATIGYYQQLGCWTPHVEITRPAFEVALDVFQHS
                     KLITRRHRYEDVIAPPPNAG"
     gene            11512..12498
                     /locus_tag="VFX87_13880"
     CDS             11512..12498
                     /locus_tag="VFX87_13880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996067.1"
                     /translation="MISRRAFVAAVTGGLLAAPLAAGAQPAGKPYRIGFLGNSTEALE
                     VNLVGPFRDGLRELGYVEGRDVVIEYRWAEGQYERFPALVEELIALKVVVLVTAGTPA
                     ALAVKRATTTVPLVMVAVGDPVGTGLVKSLARPGGNLTGLVSIAPDLEGKRLELLTEV
                     VPKLSSVAFLSNPANPFHATSEKQARAAARALHLKVEFFPVRAESEFDRAFLAISGRR
                     PGALVMLADRLFLHHRARIVEFAAHNRLPTVYAYTELVEAGGLMSFGPSYPGMHRRAA
                     YFVDRILKGGKPADLPMEQPAKFELLINLKTARALGLVIPQPVLLRADDLIQ"
     gene            12615..12845
                     /locus_tag="VFX87_13885"
     CDS             12615..12845
                     /locus_tag="VFX87_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4996068.1"
                     /translation="MPEQRVATDRLEVVRAPAHGRLEVIQPNVVAHTPSAGYTGPDEF
                     HYGGSGPGREGRTLPFSMRVQVQVVGPGAPVR"
     gene            complement(12876..14117)
                     /locus_tag="VFX87_13890"
     CDS             complement(12876..14117)
                     /locus_tag="VFX87_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996069.1"
                     /translation="MDFDDTREEAAFRAEGRQWLCAHAEPKRGAFETWQSRYPGREGW
                     DGLERAQAFQRSKAEAGFAALHWPAEWGGRALPPIYQVIYAQEESRFLVPRGYFEIGL
                     GMCMPTLFAYATDEQKRRYAPAALRGDEVWCQLFSEPGAGSDLAGLRTRAQRDGDDWV
                     INGQKIWTSGAHWADWAILVARSDPKTAKHKGLTFFFLSMKSPGIETRRIKQISGASH
                     FNEVYFTDVRIPDAQRLGAVGAGWGVAITTLMNERLTSGDLRGPDFEEIFGLARATQL
                     EDGPAIANAAVRERLADLYVKTQGVRLTRFRTMTALSRGETPGPENSIGKLVNAPKAQ
                     EIASFAMDLLDMGGVVMDKDTAPLRAAFQESLLSTPGGRIAAGTDEILRNIIAERVLG
                     LPQDVRVDRDLPFDQLPTGSR"
     gene            complement(14127..>14284)
                     /locus_tag="VFX87_13895"
     CDS             complement(14127..>14284)
                     /locus_tag="VFX87_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4996070.1"
                     /translation="KENIQVHGGMGFTWEFDCHLHYRRAKLTGLMLGSARRWKDLLVT
                     RLEAGAA"
BASE COUNT         2027 a         4900 c         5349 g         2008 t
ORIGIN      
        1 tgctcgagca ccacgcggcc gtccaccaga accgtgtcca cgctggcccc ggtctcggcg
       61 tagacgagcg ccttcaccgg atcggcgagg ggacggagga agaccgagtc ggtccggagg
      121 agcacgagat ccgccttgcg cccgggcgcg agcgcgccca ggtcctcgga ctggcccagg
      181 acgcgcgcgg ttccggacgt ggccaggccc cagacggtgt cggcgtcgag ccagcgggcg
      241 gtctcgtgcg ggaagcgcag ggtgctgatc accgaggcga tgcgcagggc ctcgaagagg
      301 ttctggttgt ccgcgcaggt ggagccgtcg gtgccgaggc ccaccgccag gccgcggtcg
      361 agcatctcgc gcaccggcgc gatgccgaca cccagccgga ggttgctccc cgggttgtgg
      421 gccaccgccg cgcccgcgtc ggccagcatc cccagatcgt cgtcgtcgag ccagatggag
      481 tgcgcgccca cgaagccggg gcccaggacg cggtgatcgg ccagccgggc cacgatgctc
      541 ttgccccagc gccgccggct ctcgaccgcc tgcaccttgg actcggccag gtgggtgtgg
      601 ataccgacac cgtgctcccg cgacagggcg gcacagccgt ccagcaactc gtcggtggcc
      661 tggttcggga tggtgggcgc caccgccacg cggacccggc cctccgcgct gccgtgccag
      721 cggcggatcg cccgctcggt gaggtcgagc aggcccttgg tgggtgacgg ccggatgtcc
      781 tggaccgtgc ggcggaggtc gggaggcagg aggtccgtga gccccgggac ggtctggtag
      841 aagacgacgt cggccaccgc gggtgccagg accacgcgga cgccgacgtc cgtgtaggcc
      901 tgcaccaccg cggcgaaggt ctcgtcggtg atcgcgggca ccgccatgta gagatcgtag
      961 gccccggtgc agcccgtctt gagcatctcg atggcgccga tggccgccga gaggtattcg
     1021 tcgtccggcg tgcgaaatcc agagtttgcc ggcgagtagg tcagcaggtc ctcgagggtc
     1081 cagctcccga cccggccccg ggccaggtgg ctggccgcat gcgtgtgggc gttgcccata
     1141 ccggggagca cgatgcggtc ggcggcctcg atcacccggg ccccggccgg agccgccagc
     1201 cccggaccca gcgcggcgat acggtcgccg tcgatcagga tatccgcccg tcgcagcgcc
     1261 gccggcgtgc ccgtcaggac ctgcccgccg cgaatcagcg ccgccatggc cgcctcctca
     1321 aaccgcgcca gagctcgaca ggggctgcgt ggccgcgtcg tcgtcgaggt gcgcgacgca
     1381 gttgagcagc cgcacgcgcc acggggccgg ggagtcgatg atcgtgagcg cggtgttctc
     1441 ccagcgctcg gggacgtcct cgccctcggg caggatcagg tggcgcgcgg ccagcgatcg
     1501 gcagaccaga ccgtgggtga cgacggccag gtgcccgctg gtggtcgtgg cctgttcctg
     1561 gacgatcgcc caggcctggt ccacgcgcgc gtggaagacg tcccaggtct ccccgttcgg
     1621 cggtgcgtag tcgagcgcga acatgtcgaa ggcgagcttg gcgtagggcg taccgcggat
     1681 gtcgccgaag ttgcgctcct ggagcagggg atcgtggcgg accgggaggc ccgtgacgcg
     1741 cgccagacgc tccgcggtct gcgcggcgcg cgagtagtcg ctcgacagga tggcgccgat
     1801 gccctcgcgc tcgagccgcc gggccaggcg ctcggcctgg gcgatgccgc gcggggagag
     1861 cgggttgtcg gggagctgga cggtgcgcga ggcgttgccc agcgtctcgc cgtgccggat
     1921 caggaagatc agatcacgct ctcgccgcgc agacgctgga tgtccgccgg gctgtatccc
     1981 agaccggcca ggatctcggc ggtatgctgg ccgagcttcg gcgccgtccg ccgcaccgcc
     2041 acgtcgctgg ccgagaggcg gatgggggtg cggatgtggg gcacgcggcc cagctcggcg
     2101 tcgttcgcgt ggaccaccat ctcgcggtgc tgcacctgag ggtcggagaa gagctgctcg
     2161 tagttgtaca cgggcccaca cggtacgccc gcgtcatcga gcaccttggt ccagtgcgag
     2221 gtcggcgcgg tcgccagcac cgcggtgatc tccttctcga gcgcgaaacg gttcttccgc
     2281 cgctccgggg agcgcgcgaa gcgcgggtcc tcgagccact ccgggtgacc gagcgcgcgg
     2341 gcgcagcgct gccagatgga ctggccggcg gcgccgatca tcatgtagcc gtccttggtc
     2401 tcgaagcgct ggtagggcgc gttctgccgg tggcgcgagc ccatgcggac gggctccgcg
     2461 tggtcggcca gccagcccgc gctggtccac gaggagaagc cgaccgcggt ctccagcagc
     2521 gagcactcga ccttctggcc caggccggtg cgctgccgct cgtagagcgc ggccagcacg
     2581 ccctgggcgc cccacatgcc ggtgccgaga tcgcagatcg gcaggcccac cgaggtgggc
     2641 gggccgtcgg gctcgccggt gacgtgcatg atgccgccca tcccctgggc gatgaggtcg
     2701 aagccgccct tctcgcggta cgggccgtcg gagccgaagc cggagagctg ggcgtagatc
     2761 aggcggggat tcacctgcct gagcgtctcc cagccgagcc cggcgcggtc catgacggtg
     2821 gggcggtagt tctccacgag cacgtccatg gaggggatca gcttgaggaa gatctcgcgg
     2881 cctggctcac ccttgtagtc gagcgtgagg ctcctcttgt tgcggttgat gtagcggaaa
     2941 tagggattgc gctcgctgcc gtcgcccatg ccgcgggagg agtcgcccgc gccgggccgc
     3001 tcgaccttga tcacgtcggc gcccatgtcg gccaggatca tggtgcagaa agggcccgcc
     3061 atgtgctcgg tcatgtcgag caccgtgacg ccgtcgagcg ggccccgcgg gttccgatca
     3121 gccatggtgg gtcgtgcctc cggcaaccta gcgcgtgtag tcacctgtta gatcgcgcgc
     3181 gtggcccagc acgtgggcca ccgcgatctg gtggaagacg gtggcgagcg ggccgaccac
     3241 gccccccgcc gagcggatgc ggcgcgcgaa cagctcctcg tccgcggcct ccaccgcctg
     3301 gatggtcgcg gcgcgattcg tctcgaactc ggcgagcagg tcggcgacgg tcatgccctc
     3361 gcgcttggcc acctggcgat cgttgtaggc gtcgttgccg cccttcatcg cgcgcgtggg
     3421 cggcgcctgc tccaccggcg cgggcggggt ccgggcgatg tcgatcagcc gcgggtagct
     3481 ccactcgatc gaggcgatgt gggccaggat ctggcggccg ttccagccgt tctcgtagcg
     3541 gccttcctcg agccgggcgg gctccatcgc gcgcacgagg gcgagcacct cgtcccggct
     3601 cgccgccagc gcggccagca gctcgcgctt cgtgggcgcg gggccgtcgg ggatttcgtg
     3661 gtagtcggcc acggggagag agtgaagcga ggcccggcgt cctgtcaagt cgccgcgcgg
     3721 ccactgctcc cgatggccac gccgcccagc accagcacga tcgcgatgac gagcgagagg
     3781 gtcagcggct cgccgagcca gacggtcgcg gtgatcacgc tgaccaccgg ggtggccagg
     3841 agtccgagcg aggtcgtaac cgcggggaga tggcggctgg cggttgcggt ggcccagtac
     3901 gcgatcgcgg tgcccacgat acccaggtag aggagcagcg tgacgaacga cggagtccac
     3961 cgagcgggcg ccggcgcggt ggtcgcgagc gcgaagggga ccaggagcgc ggtcgcgagc
     4021 agggtctccc aggggatgag cgcgaacggc gtcgagaccc atcggtgggc ccggatgtgc
     4081 aggatgctca gcgcccagag cagcgccccg gccaggatgg ccaggttgcc gagcacgacg
     4141 tcgcgatcgc cccagtcgag cgcgagcggg ttgaagagcg tgacgagccc ggccaggccg
     4201 atcagcacgc cggcgccgcg ccgcgcggtg aggggctcgc gcaggaagag cgccgcgccc
     4261 ggcgtgaccc agagcggcgt ggtgtaggcg agcaccacgg tgcggccggt gggcaccagg
     4321 cccaggcccc agctcgcgag cagattgaac ccgaccatgt ggagcagtgt gatgctcagc
     4381 agcaccggga ggtcttgccg gggagggagc gtgacgcggc cgcgccacgc ggtgagcgcg
     4441 aagagcgcga cggtcgcgat ggccgagcgc agcgtcaccg cccagagcgg cgagagcgtc
     4501 gccagcacga ccttgttgac gggccacgtg aggccccagg ccacgatcac gatgagcagc
     4561 gccaggaggc cctggcgccg ggcgctcgaa gacacgggcc gatcagccgc gacgctgcgg
     4621 gtgggcgagg gcgcgaggca tcgagcgcga tactgccgcg ggcgccggcg cgccgtcaag
     4681 gacaggccgg gatgataaga tcccgctcgg gaggtcaatc caatggtgac gctgctgacg
     4741 gagtcgggtg agcacgaggt gacgggcgcg gagccctcga ccgaggcgct ctggtgcccc
     4801 gcgaatgaag cgctgcaggc cacggggtgg gcggcgaagc ccgaggggct ctgccagggg
     4861 gaagtctgcg tgccgctgcc cgcgggccgc gagcgcgagt tcgtggacgg cggccggatc
     4921 aacctggccg cgctctggcg tcgtctcggt cggcccgccg tccggagcga gcgcgggcat
     4981 gtctgggtgc tgagcgaaag cgcgcgggag cggaaagcgg ccctcgcctc gctcgaggcg
     5041 ccggacttcg cgctgccgga tccctccggg cgcatgcacc ggctctccga ctatcgcggc
     5101 aagaaggtct tgctcgtcac ctgggcctcc tggtgaggct gccgtcgcga cctgcccggg
     5161 tggcaggcgc tccaggagga gctggcgggc gacaacttca tggtcgtcgc ggtggcgatg
     5221 gatagccgcg acggcgatcc gctgccgtgg atcgaggcgg cgaagccgac gtaccccacg
     5281 ctgatcgatc gcgagcaccg gctggccgag ttcttcggca tcgtgaacgt gccgcaggcg
     5341 gtgtggatcg acgaggccgg gcgcatcgtg cggccggcgg agccggcggg cgcgtacgaa
     5401 gggttccggc agatgaaccg cgccacacgc gagatgccgg aggaggtggc ccggcgcacc
     5461 gcgcaggcga aggccctcta cctggacgcg atccgggact gggcgcgcaa cggcgagcgg
     5521 agcgcgtacg cgttcaccgc tgagcaggcg cgcgcgcatc tgcccgcggt gaccgaggat
     5581 atcgccaccg cgcaggccct gttcaggctc gcgcagctcc tgatccggcg cggcgagcgg
     5641 ggcgaggccg agcgctggat ccgggaagcg agccggctgc atcccgactc gtggtgcatc
     5701 tggcgtcagg gcgccggcgt gaccgagctg ggcctggccg cccagccgga cttctgggag
     5761 cgggtggacg ggctcggcgc gaagcgctac tacgcgcccg tggacatgcc ggggatgccg
     5821 tagcatccgg atgaagagcc cgcgtttcgg tctcaaccgc ttcgactcgc gctcggtgga
     5881 cgcgttcgcc gtcgacgtgc gccgggccga gcagctcggc tgggacgcgg cgctccagcc
     5941 cgactcccag cttcgccgcc gcgacaccta cgtgctgctg gccgcggccg cgcgtgccac
     6001 cgagcgcatc acgctgggtc cgctgctggc caatccggtg aaccggcatc ccaccgtgac
     6061 cgcgtcctcc atcgccacca tcgacgagtt ggcgcccggc cgcgtgctgc tgggctgggg
     6121 cgtgggcgat accgcggtgc gcctggccgg cctcaggccc gcgcgggtgg ccgagctgga
     6181 agcggggacg cgcctcatgc gcgcgctgct cgccggcgaa gcggtgcagg tcggcgcggc
     6241 gcggccggcg cggctgccgc atcaccggcc ggtgccggtg tgggtcgcgg caggcggacc
     6301 gaagaccctg cggatggcgg gcggggtcgc cgatggggtc ttcatccggg taggcacgca
     6361 tgcggccaac atcgcgaccg cggtgcgcgc gatccgcgcc ggcgcggcgg aggcggggcg
     6421 cgatccgtcg gtcgtgaagc tcggcgccat cttccacacc gtgttggtgg acgagccgag
     6481 ccgcgcgctg accatggccc gctcgatggc cgccggctac tacgagtatt cgcccgcgct
     6541 cttcgatccg ccggccctcg agtggaccgg gcccgatccc gagaccctca agcgcgaccg
     6601 cggtgtgtgg cccgacttcc accacgcccc ggacctcgaa gccagcggca gggtcgtgga
     6661 tttcctcccg gtcgaggccg ccgacgcctt cagcctccgc ggaggaaccg gggaggtggc
     6721 cgagcgcctg ctcgcggcgc tgcgcgcggc gcccgccacc ttcgaccacg tggtgctgca
     6781 cccgatcccg gacccgaagt ggcccgggga cccggagaac gactacacgg cgcgcatcgc
     6841 ccgcgaggtc ctgccgcggg tccgcaagga gctcgagacg cgctagccga gctgcttgtg
     6901 gacgaactcg atgatgcgcg agatcgtccg ctccgccgcc gcgggcttct tgctgatgta
     6961 gccctcggcc tcgccctcga tcagctcgag atccacctgg ccgccgatct tccggtactg
     7021 cgccacgaag cggtccaggt cggggcgcgg gtgcgccatg tcgcgcgagc cctggatgta
     7081 gaccaccggc ggggtctcga cgcgctcgcc gcgctcgagc gcgagcgtcg ggttgccctc
     7141 ggccatggcc tcctcggtgc gccagtacgc gtcgtgaccc gggagcacgc gatccaccac
     7201 gtccggatac ggggcgccgc ccgccttcag cgccttggcg tagcggtaac gcgagagcgg
     7261 gtcgatgacc ggccagcaga gcactgcgca gcggacgctg ccgtccactg tcggcgcccc
     7321 agcgggcagg ggcagggccg cgtacttcgg atcgcgcggc ttcatggcga gcagcatggc
     7381 ctggtgcgcg ccgctggagg cgcccatgag accgacctgg tccgggcggc cgtggaactc
     7441 cgccgcgcgg ctcttgagcc agcggatcgc gtagctgatg tctaccagcg agccggggta
     7501 ggaggcctcg gggggctggc ggaagtcgag cgcggccacg accacgccgc tccgggcgag
     7561 cggctcgttc atgatggtgt cgttcgtccg gtcgccgcga ctccaggccc cgccgtgcag
     7621 ctcgacgatc atggggaatg gtccgtctcc ccggggcacg aacatccggg ccaggagagg
     7681 ccggtccccg tgacggaggt actccacgtc cttgacgtcg atctcgtgac ccatggtcgc
     7741 catggcagca ctcctttgtg agggagggtc ggtgtcggcg cgtatgatac acccagccgc
     7801 gggggcccgg ggattgccag ccggaccggc gcgccccata cttttcgcga tgccttcgac
     7861 acccggcttc ggggcgggcc acgaggtgaa agcgtgtgcg gacgggccga gcggcctcgc
     7921 ccggctccgc gccttcaagc cggacgtggc cctgatcgat ctgggcctgc ccggcatgga
     7981 cggctacgcg gtggcgcgcg aggcccgcgg gcggccggag acccgcgcga tccggctcgt
     8041 ggcggtgacc ggctacgggc aggccgagga ccggcggcgc gcactggcgg ccggcttcga
     8101 cctgcacgtc accaagccgg tggacgcctc gatgctcgac gaggtgctgg gccctgctac
     8161 gtggtgatcg ccgcgcgagt caccaccggc tcgagcccgt agacggtctg caggtgctcg
     8221 cgcgtctcga ccagggtcgt ccccacgtag ctcgcgcccg cggcctcgag gtggcgccgg
     8281 gcggtcccgt cctcctcgcg tagcccccag cgccccacga tgatccgcag ctcgggaaag
     8341 cgcgcgcgga gccgcttgct caggtagcgc gtgcgggccg cgaggccact ggcgggcagg
     8401 gcaccgaggc agacgacggc cggcccggag ctttccacga gcgcgatgac ctcggaggcc
     8461 aggagctggg gcgaggttag ctcgatcgtc cagcaggcgg ggtcgatgag atggcccagc
     8521 atgatgagtc ccagctggtc cgcctcgtcg cgcaccgggc tggcgagcag gtggatcttc
     8581 cgggtggggg gcgaggcctc gggcggcgcc gcgctggcct cggcatcggc ggcgcgctgc
     8641 tcggtctctc gcttcgcctg gaggcccagg acgatgtcgc gcgtggcgcg ccacacgtag
     8701 cgctcgtccg cctccgggag ctgcccgtgc cgccgatcgc gccgggcgcg cccgagcgcg
     8761 ggcaggatga gatcgtcgta caccgcctcg agcggatggt ccttcgcgta ttcggcggcg
     8821 atgcgcgcgg cctcggactc gtcctccgcc agcaggcgct gataataggc gacgggcggg
     8881 gcaatggaag gcacgtcggc catcaggatg ccgatgaact ccatcccggg cacgtgcttg
     8941 gcgaagacga ccaggcacac ggtgagcggc gtggcgagga gcagtcccac cgggccccag
     9001 agccaggccc agaaggcgac cgagcagagc agcgccacct gggagacgcc cgcgctctgc
     9061 ccgtagagca agggctccag cgccatgctg cacagcagct cgagcagcac gaagagaccg
     9121 gcgacgagca acggctggtg ccaggtcggg aaggcggcga gggcgaacac gatgaggatc
     9181 agcgcggcca gccaggcgcc cacgtagggg atgaagcgca gcactgcggc caggaagccc
     9241 cagaggaggg cgtagggcac ccccagtagc agcaggccca ctgcgatgcc gagcccgaag
     9301 ctggcgttga tgatcgactg catcagcagg tagcggctga ttcgctgcgc ggcctcgtcc
     9361 agcgcgcggg tcgtcgtggc gagcttccgg tagccgacga gtcggagtac ccgattccgg
     9421 agctccgcct gctcgatgag catgaagatc acgagcacga ggaccgtcgc ggcaccgccc
     9481 agcccctcga tgagcgtggg gagctgccag aggccggtcg gctgcgggcg gaccaccacc
     9541 gggggcggcg tgccccgggg cggggccggc gtcgtggggt ccttttgcag ctccttcgtg
     9601 acctcggtca ccgcgctctg cgccttctcg agcacgcccc cgcggcccat gcggcggacg
     9661 tatgcgatct tcgcgatcag gttgtcgcgg tagcccggga tgccggttga cagcagcgtg
     9721 agctccgcgg cgaggcccca cgccaccacg ccgagcgtgg caaacaacat cgtcacgacg
     9781 atgcccaccg acagggcgcg tcccaggccg aggcgcgcga gcggtgcgac cactgggtgg
     9841 accaggaagg tcagcaggat cgcgagcgcg atcgggatca acactccgcg cgcgaggtag
     9901 agcgcggcca cgcccacgac cagggcggcc atcaccacga gcggcttgac cgagcgcgcg
     9961 gattccgcca cgagcccagg agggagcaag cgatgtgcca cgccgcccgg gggcgaacta
    10021 gcgcggaaat cgccccgtga ccttcgattc cggtagtttc ttcgcggtaa gtccgacgcg
    10081 gcccgtctgg gggctagacc cgagaactga tggcgaccag acctaggggc caggcttctc
    10141 cttctcgaag agcggcaggt acttgccgta cccctccgcc tcgagcttgg cgacggggat
    10201 gaatcgcagc gccgcggagt tgatgcaata gcgcaggccg gtcgagggcg ggccgtcggg
    10261 gaagaggtgc ccgagatggg aatcaccctc ggcggagcgg acctcgaccc ggcgcatgcc
    10321 gtaggaattg tcggcgtgtt ccttgaccgt ctcggcttcg agcggccggt cgaagctcgg
    10381 ccagccggtg ccggagtcga acttgtgcag cgacgagaac agcggctcgc ccgtcaccac
    10441 gtccacgtaa atgcccgcct cgtggtggtt ccagaactcg ttccggaacg gcggttccgt
    10501 ggcgctgcac tgggtaacgt tgtattgttc cggggtcaaa cggctcttga gatccgcgtc
    10561 ggtcgggcgc ttggtggtgg ccatggcacc cgtgagtata accctgggag ggatgtcatg
    10621 agtgacgaga tcagcatcca gttcacccgc ttctccgcct tctactcccc gctgatcgcc
    10681 acgatcgcgg gcggcttcct ccagcaggag ggcttcgcgc ccaagcactc catcgcgccg
    10741 gccggcaagt cggccatcga gggggtcgtg gccggcaccg tgcacgtgtg ccagtccgcg
    10801 ccgtcgcagg gcttcggccc gctcgagaag ggccaggccc cgcccgtggt gcacttcgcc
    10861 cagatcaacg agatggacgg gttcttcctg accggccgcg cgccggaccc ggcgttcacc
    10921 tgggacaggc tgaaagggcg gcgcgtgctg gtggaccacg gcggccagcc gctggcgatg
    10981 ttcaagtacg cgtgccacaa gcgcgggctc gacttcgcga gcatcgtggc cgtgaacgtg
    11041 ccgagtgatc agatggacgc ggcgttccgg aagggcgagg gcgactacat ccaccagcag
    11101 gggcccgcgc cccagcagct cgagcacgac cacgccggcc acgtcgtcgc ctcggtgggc
    11161 caggccatcg ggccggtggc gttctccagt ctggccgcca cgcgcgcgtg gctggccacc
    11221 gacatggccc ggcgcttcat gcgcgcatac cggaaggcgc gcgcgtggct gctggccacg
    11281 cccgcggcgg aggtcgcgaa ggcggaggcc tcgttcttcc ccgagatcga tcaggcggtg
    11341 ctcgccgcga cgatcggcta ctaccagcag ctcggctgct ggacgccgca cgtcgagatc
    11401 acccggcccg cgttcgaggt cgcgctcgac gtcttccagc actcgaagct gatcacccgg
    11461 cggcatcgct acgaggacgt gatcgcgccc ccgcccaatg cgggctgacc gttgatctca
    11521 cgccgggcgt tcgtggcggc ggtgaccgga ggcctcctcg cggcgccgct cgccgcgggg
    11581 gcgcagcccg ccgggaagcc ataccggatc gggttcctcg gcaattccac ggaggcgctc
    11641 gaggtcaatc tcgtcggacc gttccgggat gggctgcgcg agctcgggta cgtcgaaggc
    11701 cgagacgtcg tgatcgagta ccggtgggcg gaaggtcagt atgagcgctt tcccgcgctg
    11761 gtcgaggagc tgatcgccct caaggtggtg gtgctcgtca ccgcgggcac acccgccgcg
    11821 ctggcggtca agcgggcgac gacgaccgtc ccgctcgtca tggtggcggt cggcgatccg
    11881 gtcggcaccg ggctggtcaa gagcctggct cggcccgggg gaaacctcac cgggctggtg
    11941 tcgatcgcgc cggatctgga gggaaaacgg ctggagctgc tcacggaagt cgtgccgaag
    12001 ctctcgtccg tggccttcct ctccaatccg gccaacccgt tccacgcgac ctcggagaag
    12061 caggcgcgcg cggcggcccg ggccctccac ctgaaggtgg agttcttccc ggtgcgggcg
    12121 gaatcggagt tcgaccgcgc gttcctggcc atcagcggcc ggcggccggg ggcgctcgtc
    12181 atgctggccg accgtctctt cctgcatcac cgggcgcgga tcgtggagtt cgccgcgcac
    12241 aaccgccttc cgacggtgta cgcgtacacg gaactggtcg aggccggggg cctgatgtcc
    12301 ttcggcccga gctatcccgg catgcaccgg cgcgccgcct acttcgtgga ccggatcctc
    12361 aagggcggca agcccgccga tcttccgatg gagcagcccg cgaagttcga gctgctcatc
    12421 aatctcaaga ccgcgcgggc actcggcctg gtgattccgc agccggtcct gctgcgggcg
    12481 gacgacctga tccagtgatg gccgggcgac ctgtgcgcgg tgtccattgg gcgtggcgcc
    12541 accggagtgg gcggggccgc cgggaccatg acggtgcgca acgccgggcg gcctgccgca
    12601 tcacgaactt cacggtgcct gaacagcgcg tcgccaccga ccgcctcgag gtcgtccggg
    12661 ccccggcgca cgggcggctc gaggtcatcc agcccaacgt cgtcgcccac actccgagcg
    12721 ccggctacac gggcccggac gagttccatt acggcgggag cggccccggc cgggaaggcc
    12781 ggacgctgcc cttcagcatg cgagtccagg tgcaggtcgt gggccccggc gccccggtgc
    12841 gctagggaag ggggcgtacg cccccgccga gtcactcacc gtgagccggt cgggagctga
    12901 tcgaagggga gatcgcggtc cacgcgcacg tcctggggta ggccgagcac gcgctccgcg
    12961 atgatgttgc gcaggatctc gtcggtcccc gccgcgatgc gtccgccggg cgtggagagc
    13021 agcgattcct ggaaggcggc gcgcagcggc gccgtgtcct tgtccatgac cacgccgccc
    13081 atgtcgagca ggtccatcgc gaacgaggcg atctcctgcg ccttgggcgc gttgacgagc
    13141 ttgccgatgg agttctcggg ccccggcgtc tccccgcgcg acagcgcggt catggtgcgg
    13201 aagcgggtca ggcgcacgcc ctgggtcttc acgtagaggt cggccaggcg ctcccgcacc
    13261 gccgcgttcg cgatggcggg gccgtcctcg agctgggtgg cccgggccag gccgaagatc
    13321 tcctcgaagt cggggccgcg caggtcgccg gaggtgaggc gttcgttcat gagggtggtg
    13381 atggccacgc cccagcccgc gccgaccgcg cccaggcgct gggcgtcggg gatgcgcacg
    13441 tcggtgaagt agacctcgtt gaagtgcgac gcgcccgaga tctgcttgat ccggcgggtc
    13501 tcgatgccgg gcgacttcat ggacaggaag aagaaggtga ggcccttgtg cttggccgtc
    13561 ttcggatcgc tgcgcgcgac caggatggcc cagtcggccc agtgcgcgcc cgaggtccag
    13621 atcttctggc cgttgatcac ccagtcgtcg ccgtcccgct gggcgcgggt gcgcaggccg
    13681 gccaggtcgg agccggcgcc cggctcggag aagagctggc accagacttc atcgccgcgg
    13741 agggccgcgg gggcgtagcg ccgcttctgc tcgtcggtgg cgtaggcgaa gagggtcggc
    13801 atgcacatgc cgaggccgat ctcgaagtaa ccgcgcggga cgaggaagcg cgactcctcc
    13861 tgagcgtaga tcacctggta gatcggcggc agggcgcggc cgccccactc ggcgggccag
    13921 tgcagcgccg cgaagccggc ctccgccttg ctccgctgga aggcctgggc gcgctcgagc
    13981 ccatcccagc cctcgcgccc cgggtagcgg ctctgccagg tctcgaaggc cccgcgcttg
    14041 ggctccgcgt gggcgcagag ccattgccgc ccctccgcgc gaaacgccgc ttcttcccgg
    14101 gtgtcgtcga agtccacggt gcgctcctag gccgccccgg cctcgagccg ggtgacgagc
    14161 agatccttcc agcggcgggc gctgcccagc atgaggccgg tgagcttggc gcgccggtag
    14221 tggaggtggc agtcgaactc ccacgtgaag cccatgccgc cgtgcacctg gatgttctcc
    14281 ttgg
//
LOCUS       DASUFA010000379         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1593174, whole genome shotgun sequence.
ACCESSION   DASUFA010000379 DASUFA010000000
VERSION     DASUFA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593174"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFX87_13900"
     CDS             complement(<1..483)
                     /locus_tag="VFX87_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4996071.1"
                     /translation="MNAQSVPDPEQPLPGPRRAGPLAGLRVLDCSSLIAGPTAAMYLG
                     DYGADVIKIEHPQGDGLRVWGAVKDGAGLYFKMLNRNKRSITADLHTPLGAEIARRLA
                     ARADAMIENFRPGTLEKWGLGYETLATVNPGLVLTRVTGFGQTGPERDRPGFGTTAEA
                     A"
     gene            567..1337
                     /locus_tag="VFX87_13905"
     CDS             567..1337
                     /locus_tag="VFX87_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX4996072.1"
                     /translation="MARVLDILETLSGLPGGASLTELSVALEAPKTSLLPLLRTLVSR
                     GYAARSDSGRYGIGPRWLEAGDRGAVAPDLVEAAHPVLQALTRETGETTFVAVLPPGH
                     ADLVYVDKVESPQRIRYSAELGERRPLYCTATGFGLFAFLEPARREHLVHALPLPRHT
                     RRTTTSRDLLRRRLDAIRAAGVAVTVDEFVIGAGGAAAPVYGRDGRLVAACAVTGPTA
                     RIQAGRRRFAEAVKRAAAEISERLGYHRMEKGTEKRAR"
     gene            1334..2368
                     /locus_tag="VFX87_13910"
     CDS             1334..2368
                     /locus_tag="VFX87_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996073.1"
                     /translation="MSETDEPADPAALIEAYYDAGWTDGLPVVPPSDASIAAMLAAAG
                     LQGHEELGAITGRNAVVTADKAAINAVMAGCRPEYMPVVVAAVKSLCAPAFAYHGPAS
                     STGGSAMALVVNGPIASRLAINSGNNAFGQGHRPNATIGRAVRLIMMNVMNTRPGLLD
                     RATLGNPGKYTFCFAEHEEDHPWEPLHVARGLRREDSAVTLIASNSLYQVYNHLAAEP
                     EPLLYCFADALGNVGTSNLLGFNETLIVFAGEHQEVLQKAGWSRLDVQRFLMDRTRRT
                     VADYKRGARLPGEVTPADETTWRYRFERPEDILIAFAGGRAGSWSACLPGWGTKWTRS
                     VTTRIEGGSA"
     gene            2365..2643
                     /locus_tag="VFX87_13915"
     CDS             2365..2643
                     /locus_tag="VFX87_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996074.1"
                     /translation="MKLFDPTSKPVGRAIKLAPRPASPRGLRLGLVENTKFNASPILE
                     KLAERLARRHQMTVTVRNRKRSASHEVDGDGIKALRAQADLVISGIGD"
     gene            2665..2892
                     /locus_tag="VFX87_13920"
     CDS             2665..2892
                     /locus_tag="VFX87_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996075.1"
                     /translation="MLDAILMEEQGVPAIAIVTTPFRNTGRAMAESWGKPGYPFLDMP
                     HPIANLTPAQLDERCDRLADQVEEWLGFEAH"
BASE COUNT          429 a         1011 c         1106 g          394 t
ORIGIN      
        1 ggccgcctcg gcggtggtgc cgaagccggg gcggtcgcgc tcgggcccgg tctgcccgaa
       61 gccggtcacc cgcgtgagga cgagccccgg gttgacggtg gccagcgtct cgtagcccag
      121 gccccacttc tcgagcgtgc cggggcggaa gttctcgatc atcgcgtcgg cgcgcgcggc
      181 cagccgccgc gcgatctcgg cgccgagggg ggtgtgcaga tcggcggtga tcgagcgctt
      241 gttgcggttg agcatcttga aatagaggcc ggcgccgtcc ttgaccgccc cccagacgcg
      301 cagtccgtcg ccctgcgggt gctcgatctt gatcacgtcg gccccgtagt cgcccaggta
      361 catcgcggcg gtggggcccg cgatcagcga ggagcagtcg agcacgcgca ggccggccag
      421 cgggcccgcc cggcgggggc ccggcagggg ctgttccgga tccggaaccg attgcgcatt
      481 cacgaactgc catgctacgg tctttggcga tggtgtcaag gggcgcggcg gcgcgtgggg
      541 cttcgcggga cggcggtcac cgggcggtgg cgcgggtgct ggacatcctg gagacgctct
      601 cgggtctgcc cgggggcgcg agcctcaccg agctgagcgt ggcgctggag gcgcccaaga
      661 ccagcctgct cccgctgctg cgcacgctgg tgagccgcgg ctacgcggcg cggagcgact
      721 ccgggcgcta cgggatcggg ccgcgctggc tcgaggccgg cgacaggggt gccgtcgcgc
      781 cggacctggt cgaggccgcc cacccggtgc tgcaggcgct cacgcgcgag accggcgaga
      841 ccaccttcgt ggcggtgctg ccgcccggcc acgcggacct ggtctacgtg gacaaggtgg
      901 agagcccgca gcgcatccgc tacagcgcgg agctgggcga gcgacgcccg ctctactgca
      961 ccgccaccgg cttcggcctc ttcgccttcc tcgagcccgc gcggcgcgag cacctggtgc
     1021 acgcgctgcc gctcccgcgc catacgcggc ggacgacgac gagccgcgat ctgctccggc
     1081 ggcgcctcga cgccatccgc gccgcggggg tggcggtgac ggtggacgag ttcgtgatcg
     1141 gggcgggggg tgcggccgcg ccggtgtacg ggcgtgacgg ccgcctcgtc gccgcgtgcg
     1201 cggtgaccgg cccgaccgcg cgcatccagg ccgggcgccg gcggttcgcg gaggcggtga
     1261 agcgcgcggc cgccgagatc tcggagcggc tgggctatca tcgaatggag aagggaacgg
     1321 agaagagagc gcgatgagcg agaccgacga gccggcggac ccggccgcac tgatcgaggc
     1381 gtactacgac gcgggctgga ccgacgggct gccggtggtg ccgccgtcgg acgcgtccat
     1441 cgcggccatg ctggccgcgg cggggctcca gggccacgag gagctgggcg ccatcaccgg
     1501 ccgcaacgcg gtggtcaccg cggacaaggc ggcgatcaac gcggtgatgg cgggctgccg
     1561 ccccgagtac atgccggtgg tggtggccgc ggtgaagagc ctctgcgcgc ccgccttcgc
     1621 ctatcacgga ccggccagca gcaccggcgg ctccgcgatg gcgctggtgg tcaacggccc
     1681 gatcgcgtcg cggctcgcca tcaacagcgg caacaacgcg ttcggccagg gccaccggcc
     1741 caacgccacg atcggccgcg cggtgcgcct catcatgatg aacgtgatga acacgcgccc
     1801 gggtctgctc gaccgcgcca cgctcggcaa tcccggcaag tacaccttct gctttgcgga
     1861 gcacgaggag gatcatccgt gggagccgct gcacgtggcg cgcggcctgc ggcgcgagga
     1921 cagcgcggtc accctgatcg cgtcgaacag cctctaccag gtgtacaacc acctggccgc
     1981 ggaaccggag ccgctcctgt actgcttcgc tgacgcgctc ggcaacgtgg gcacttccaa
     2041 cctgctcggt ttcaacgaga cgctcatcgt cttcgcgggc gagcaccagg aggtgctgca
     2101 gaaggcgggc tggagccgac tcgacgtgca acgcttcctg atggaccgca cgcgccggac
     2161 cgtggccgat tacaagcgcg gggcgcgcct gcccggtgag gtgacgcccg cggacgagac
     2221 gacctggcgc tatcgcttcg agcggccgga ggacatcctg atcgcgttcg cgggcggccg
     2281 ggccgggagc tggtccgcct gcctgccggg gtggggcacc aagtggaccc gcagcgttac
     2341 cacacgcatt gaaggaggca gcgcatgaag ctcttcgatc cgacctcgaa gccggtgggg
     2401 cgcgcgatca agctcgcgcc ccggcccgcg agcccgcgag gcctgcgcct cgggctggtg
     2461 gagaacacca agttcaacgc cagccccatc ctcgagaagc tcgcggagcg gctggcccgg
     2521 cgccatcaga tgacggtcac ggtgcggaac cggaagcgct cggcgagcca cgaggtggac
     2581 ggggacggga tcaaggcgct gcgcgcgcag gccgatctcg tcatctccgg catcggcgac
     2641 tgagggtcgt gcagctcgag cagtgtgctc gacgcgatcc tgatggagga gcaaggggtg
     2701 ccggccattg ccatcgtcac caccccgttc cgcaacaccg ggcgagcgat ggccgagagc
     2761 tggggcaagc cgggctaccc gttcctggac atgccacatc cgatcgccaa tctgacgccc
     2821 gcgcagctcg acgagcggtg cgatcggctg gcggatcagg tcgaggaatg gctcgggttc
     2881 gaggcgcact aacacgtaag atgtcggcgt gagccgtatc cgctacgatc ggctgcccac
//
LOCUS       DASUFA010000380         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_775035, whole genome shotgun sequence.
ACCESSION   DASUFA010000380 DASUFA010000000
VERSION     DASUFA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775035"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1865
                     /locus_tag="VFX87_13925"
     CDS             78..1865
                     /locus_tag="VFX87_13925"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4996076.1"
                     /translation="MTAVVFAPVLRAGFVDWDDPINFLENPSYRGLGWRQLRWMLTAN
                     VMGHWIPVTWLTLGADFTVWGMNPFGYHLTNLLLHTVSAALFYLVSRRLLGLAMPAAS
                     PGTVSLGASVAALYFAVHPLRVESVAWITERRDLTSGLFFLLTILAYLKAHERPPAVR
                     MAWRWVSLGAATLALASKSIVMGLPLVLLILDIYPLRRLGPRVRDWWRAQAWPVWREK
                     IPFALLALGTAAAAYLVQRSTGYLTPVDPAGRIGMVAYNVWFHVWKTVAPLNLGPLYE
                     LPPRMNPLEPPYLLSAAGALAITATVWLLRRRWPAGLAIWAFYLVMLAPVAGFVHTGN
                     HLGADRNTYVPGMGFALLVGALVMTIVLAWRRGLLRAPIVAMALGVVAIWLGGLALTA
                     RVQSSIWHDSQTLWRYAIEVDPDCAICHHNLGIILGSRGDWADAQALLERAIALRPDR
                     SEFHGNYGPLLIQMGRRPEGVAKLRYRLAHDPRDVNARVNLGIALIEDGRPGAAILEL
                     EQALRVKPDSVPALTSLGRALLAEGHAEPAQAAFERALAINAADPVAHLGLARTHLAR
                     GDRAAAREQIPILDRLDPRLARLLEQEMR"
     gene            1941..>3173
                     /locus_tag="VFX87_13930"
     CDS             1941..>3173
                     /locus_tag="VFX87_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4996077.1"
                     /translation="MAAGSNPSRIGVDIGGTFTDLVWVDETTGAVRVGKLLTTPKDPS
                     QAVEEGVVTLLHEAGAATTAVRALIHGTTLATNALIERKGARVGLLTTAGFRDAVEIG
                     REGRYDMYDLFIDPPVPLVPRQLRVEVSERVQADGSVLRPLDEAQARAAVAQLGALGV
                     EAIAICLLHAYRNPAHERALARLCAELAPGVPVSCSSDVVPEIREYERTSTTTANVYV
                     MPLMARYLDDLERKLQGLGLPGRLYVMMSAGGIATPETAKRVPIRLVESGPAAGALAA
                     ARMARQVGMDRVLSFDMGGTTAKACVIDRGEPLLAREFEVARADRFKKGSGLPIRVPV
                     VELIEIGAGGGSVARVDRMGLLKVGPDSAGADPGPACYGLGGREPTVTDADLLLGYLD
                     ADFFLGGRMRLDVEAARRA"
BASE COUNT          399 a         1186 c         1083 g          505 t
ORIGIN      
        1 gatgcttttt cgactcggtt agactggtcg ggatgttgcg tcccgcctgg cgggctgcgc
       61 cactcgctct cgccctcgtg accgccgtgg tcttcgcccc cgtcctccgc gccggcttcg
      121 tggactggga cgaccccatc aatttcctgg agaacccgtc ctaccgggga ctcggctggc
      181 ggcagcttcg ctggatgctg accgcgaacg tgatgggcca ctggatcccg gtgacctggc
      241 tgacgctcgg cgccgacttc acggtatggg gcatgaaccc gttcgggtac cacctcacga
      301 atctcctgct gcacaccgtc agcgcggcgc tcttctatct cgtctcgcgc cgcctgctgg
      361 gcctcgcgat gccggcggcc tctcccggaa ccgtgagcct cggcgcgtcc gtcgccgcgc
      421 tctacttcgc ggtgcacccg ctacgggtgg agtcggtcgc gtggatcacc gagcggcgcg
      481 acctcacgtc gggcctgttc ttccttctga cgatcctcgc gtacctcaag gcccacgagc
      541 ggccgcccgc ggtgagaatg gcctggcgct gggtgtcgct gggcgcggcc acgctggcgc
      601 tcgcctccaa gtccatcgtg atgggcctgc cgctggtcct gctcatcctc gacatctacc
      661 cgctgcggcg gctcggcccc cgcgtccggg actggtggag ggcccaggcc tggccggtct
      721 ggcgcgagaa gatcccgttc gcgctcctcg cgctcggcac cgccgccgcg gcgtatctgg
      781 tccagcggag cacgggctac ctgacccccg tcgacccggc cggccggatc ggcatggtcg
      841 cgtacaacgt gtggttccac gtctggaaga cggtggcgcc gctcaacctg gggcccctct
      901 acgagctgcc tccgcgaatg aatccgctgg agccacccta cttgctgagc gcggccggcg
      961 ccttggcgat caccgccacg gtatggctcc tgcggcggcg ctggccggcg ggcctcgcga
     1021 tctgggcctt ctacctggtg atgctcgccc cggtggccgg gttcgtccac accggcaatc
     1081 acctcggagc agatcgcaat acctacgtgc ccggcatggg attcgcgctg ctggtcggcg
     1141 cgctcgtgat gacgatcgtc ctggcatggc gccgtggcct gctgcgcgca cccatcgtgg
     1201 ccatggctct gggcgtcgtg gcgatctggc tcggcggcct cgcgctcacc gcgcgggtcc
     1261 agtcctccat ctggcacgac tcgcaaaccc tctggcgcta cgccatcgag gtggatcccg
     1321 actgcgccat ctgccaccac aacctcggca tcatcctcgg cagtcgcggc gactgggccg
     1381 atgcgcaggc tcttctggag cgcgcgatcg cgttgcggcc cgaccggtcc gagttccacg
     1441 gcaactacgg gcctctcctg atccagatgg gccggcgccc ggagggcgtg gccaagctcc
     1501 gctaccgcct cgcccacgac ccgcgggacg tcaatgcccg cgtcaatctg ggaatcgccc
     1561 tcatcgagga tggccggccc ggcgcggcga tcctcgagct ggagcaggcg ctgcgcgtca
     1621 agccggactc cgtgccggcc ctgacctccc tcggccgggc actcctcgcc gaaggccacg
     1681 cggagcccgc gcaggcggcc ttcgagcggg cgctcgcgat caatgcggcg gatcccgtcg
     1741 cccatctcgg actcgcccgc acgcacctcg cgcggggcga tcgcgccgcc gcccgggagc
     1801 agatccccat tctggatcgt ctcgatcccc ggctcgcccg tctcctcgag caggagatgc
     1861 ggtaggagcg gaccggtttc ggtgtagact tcccctcgca acgattcaac ggcagcgatc
     1921 ccccacatga ggtcagcgac atggcggccg gctccaaccc ctcgcggatc ggcgtggaca
     1981 tcggcggcac tttcaccgac ctggtctggg tggacgagac caccggcgcc gttcgcgtcg
     2041 gcaagctcct gaccacgccg aaggacccgt cgcaggcggt ggaggagggc gtggtgaccc
     2101 tgctgcacga ggcgggcgcc gccaccaccg cggtccgcgc gctgatccac ggcaccacgc
     2161 tggccaccaa cgcactgatc gagcgcaagg gcgcgcgggt ggggctgctc accaccgccg
     2221 gattccggga cgcggtcgag atcgggcggg aaggccgcta cgacatgtac gacctcttca
     2281 tcgacccgcc ggtgccgctc gtgccgcgcc agctccgcgt ggaggtgtcc gagcgcgtgc
     2341 aggcggacgg ctccgtgctc cgcccgctcg acgaggctca ggcgcgcgcc gcggtggccc
     2401 agctcggcgc cctgggcgtg gaggccatcg cgatctgcct gctgcacgcc taccggaacc
     2461 cggcgcacga gcgggcgctc gcccgactct gcgcggagct cgcgcccggt gtgccggtat
     2521 cctgctcctc cgacgtggtc cccgagatcc gcgagtacga gcgcacctcc accaccaccg
     2581 ccaacgtgta cgtgatgccg ctgatggccc gctacctgga cgatctcgag cgcaagctcc
     2641 aggggctcgg cctcccgggt cggctctacg tcatgatgtc ggccggcggc atcgcgaccc
     2701 ccgagaccgc caagcgcgtg ccgatccggc tcgtggaatc ggggcccgcg gcgggggcgc
     2761 tcgcggcggc gcgcatggcc cgccaggtcg gcatggaccg ggtgctgtcc ttcgacatgg
     2821 gcggcaccac cgccaaggcc tgcgtcatcg accgcggcga gccgttgctc gcacgcgagt
     2881 tcgaggtcgc gcgcgcggac cggttcaaga aaggctctgg cctccccatc cgcgtgccgg
     2941 tggtggagct aatcgagatc ggggcgggcg gcggctccgt cgcccgggtc gaccgcatgg
     3001 ggctgctcaa ggtcggcccc gacagcgcgg gggcggatcc gggcccggcg tgctacgggc
     3061 tgggcggccg cgagcccacc gtcaccgatg ccgacctgct cctgggctat ctcgacgccg
     3121 acttcttcct gggtggccgc atgcggctcg acgtggaggc ggcccgccgc gcc
//
LOCUS       DASUFA010000381         4957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1139063, whole genome shotgun sequence.
ACCESSION   DASUFA010000381 DASUFA010000000
VERSION     DASUFA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139063"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..939
                     /locus_tag="VFX87_13935"
     CDS             103..939
                     /locus_tag="VFX87_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4996078.1"
                     /translation="MALSSALCAASAALLIQRGLRGSTVYAGAWINVVVGATAAWTAA
                     LLLVPIEDYTWQAVPYFALSGLIGTTGGRLFRVLAIQKVGAPVAAAVNNMTPLVASGL
                     AILLLGERVTLPIVGGTVVIVAGTILLSLSGRYVGFRARDLLYPFIAASCFGSVQILR
                     KLGLASAGPLLGATINVTAALIATTAFVAASGNLGSLRCSRRSLLYFIGGGIAENGGV
                     FLLLLALGFGDVSVVIPLAGTAPLFVLALAYLIPSERSKLGWRIVAGVALVVLGVSLL
                     FQ"
     gene            988..1887
                     /locus_tag="VFX87_13940"
     CDS             988..1887
                     /locus_tag="VFX87_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4996079.1"
                     /translation="MDSARWLLPLAILGPLLVAAGALAWLLAVRLRRAEAALGRVKAE
                     IDERVRARTSELAASERQHRRLIEDSVEGIIIHRLGLIRFANAAALRLFGFTDPAQVV
                     GRGGLDLIAPEHRDRIAARMDARSRGEPTPATVELEAIRQDGSRFWLGATASAVEWEG
                     GPATLVSFIDISDRQRRETAEREAESLRSVAKLANAAAHEINNPLTVVGGNLQLLAER
                     LGDRPDLARYFERGERAVRQIADMISHMTRITRLTPLTGVETGGVPTLDLRRSSEPAP
                     PADPGEATSARPVSAPKKPDTGA"
     gene            1944..3086
                     /locus_tag="VFX87_13945"
     CDS             1944..3086
                     /locus_tag="VFX87_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX4996080.1"
                     /translation="MRELTLVGPGSPMGELLRRYWHPVGLVGDAGETPKALRVLGEDL
                     VLFRDRRGRPGLVTARCAHRGTTLYYGRVEERGIRCCYHGWLFDVEGRCLEQPCEPDG
                     GPQRERMRQPWYPVQERYGLIFAYMGPPARKPVLPRYEVLETLDPGEFLEADDSSVGG
                     GGPAVIPCNWLQHYENLMDTLHVPILHGSFSGPQFTAPMGLMPEVTWALTDKGVKAIS
                     DRRLPDGTVLHRISEAVLPTLRVIPNPRVAQYARVETIGWVLPLDDMRFRIYTAGRVR
                     KAGEIASVRSRLNGKLWEELTPAEHRDFPGDYEAQVGQGPIALHSEENLVSSDRGVAL
                     VRRQLKQQLDALAEGRDPIGVSFDPAAPPVPFEAGNFLIERQPHAS"
     gene            3076..3822
                     /locus_tag="VFX87_13950"
     CDS             3076..3822
                     /locus_tag="VFX87_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4996081.1"
                     /translation="MLRDHPTGDYRYMPGISAFSCGTVATPGHEIVHVTLAAPVPWRD
                     GFARIERHLREVKRPKTALCGIELRSPAPFTFDGFAGFNEGYRKLLAEWGILVGEDNP
                     IPRTNVAPVAAAPQEPSLYAFSYTQPGATPEPTFIVAGAGELRDRAQGPEGIVRHGDT
                     SPDGMREKARFVMAIMQDRLRTLGGDFRRVTTIDIYTAQPIHALVLDEILKAAGPAAI
                     HGVHWYPSRPPIQGLDYEMDMRGVARELVL"
     gene            complement(3920..>4957)
                     /locus_tag="VFX87_13955"
     CDS             complement(3920..>4957)
                     /locus_tag="VFX87_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4996082.1"
                     /translation="RVTPLTQPPGPALRRALTTLDPGEEWLLEPRSPSREAPLWSPGI
                     KLGVRRGAFFHRTECFGPVLGLMRAADLDEAIALANDQPFGLTSGIQSLDDREIARWL
                     ERIEAGNLYVNRHITGAIVGRQSFGGWKASSVGPGAKAGGPNYVLQLCRARPVAPPAE
                     GPAAVLRAAGAGYECAWREHFAPAHEPIRLLGERNVFRYRPCRRVLVRGEVATADGRL
                     ALSLALLAARTCGAPVTVSLSAGEAGPWLGEERAVVAVTETEGELIARLGGEGVERLR
                     ALVALSRELRVAAHAAGIVVLDAPVVAAGRIELRHYVREQSVSHLVHRYGSVPSEPLT
                     LPSPLWGEGSS"
BASE COUNT          662 a         1872 c         1770 g          653 t
ORIGIN      
        1 tccgtggcgg cgatgacgga gcagctcgcc acgctgcacg accgcctccg cgccgaagtc
       61 ggctgatccg tcgagtccgc gttgctcccg acgccacacg tgatggcgct gtccagtgcc
      121 ctgtgcgccg cgtcggcggc cctgctgatc cagcgcgggc tgcggggttc gaccgtctac
      181 gcgggcgcct ggatcaacgt ggtggtgggc gcgaccgccg cgtggacggc cgccctcctg
      241 ctcgtcccca tcgaggacta cacgtggcag gcggtcccct acttcgccct gtcgggcctg
      301 atcggcacca ccggcggccg gctcttccgc gtcctcgcca tccagaaggt cggcgcgccg
      361 gtggcggccg ccgtcaacaa catgaccccg ctggtggcca gcgggctggc catcctgctc
      421 ctgggcgagc gggtgaccct gcccatcgtc gggggcacgg tggtcatcgt cgcgggcacc
      481 atcctcctgt cgctgagcgg gcgctacgtg gggttccgcg cccgcgacct cctgtatccg
      541 ttcatcgcgg cctcgtgctt cggctcggtc cagatcctgc gcaagctcgg gctggcctcg
      601 gcggggccgc tcctcggcgc caccatcaac gtcaccgcgg ccctgatcgc gacgaccgcg
      661 ttcgtggccg cctcggggaa cctgggctcg ctgcgctgca gccggcggag cctgctctac
      721 ttcatcgggg gcggcatcgc ggagaacggc ggcgtcttcc tcctgctgct ggccctgggc
      781 ttcggcgacg tgagcgtagt gattcccctg gccggcaccg cgccgctgtt cgtgctggcg
      841 ctggcctatc tgatcccgag cgagcggagc aagctggggt ggcggatcgt cgcgggcgtg
      901 gccctggtcg tgctgggcgt gagcctgctc ttccagtgac cgccggcggg gcttgacccc
      961 acgaccggca catgagaaca ctcggcgatg gactccgcgc gctggctgct gcccctcgcc
     1021 atcctgggcc cgctgctcgt cgcggcgggg gccctcgcgt ggctcctggc ggtgcggctg
     1081 cgccgggcgg aagcggcgct ggggcgagtc aaggccgaga ttgacgagcg ggtgcgcgcc
     1141 cggacgagcg agctggccgc gtcggagcgg cagcatcgcc gcctgatcga ggactcggtc
     1201 gagggcatca tcatccaccg gctcgggctg atccgcttcg ccaacgcggc ggcgctgcgg
     1261 ctcttcgggt tcacggaccc ggcccaggtg gtgggccggg gcgggctgga tctcatcgcc
     1321 ccggagcatc gcgaccggat agccgcgcgc atggacgctc gatcgcgggg ggagccgacg
     1381 ccggcgacgg tcgagctgga ggccatccgg caggacggga gccgcttctg gctcggggcg
     1441 accgcgtcgg cggtcgaatg ggagggaggg ccggccaccc tggtctcctt catcgacatc
     1501 agcgaccggc agcgccgaga gaccgcggag cgggaagccg agagcctccg ctcggtggcc
     1561 aagctggcga acgccgcggc ccacgagatc aacaacccgc tcaccgtggt cggcggcaac
     1621 ctgcagctcc tcgccgagcg gctcggcgac cgcccggacc tggcccgcta cttcgagcgc
     1681 ggggagcggg ccgtccgcca gatcgccgac atgatcagcc acatgacgcg catcacccgg
     1741 ctcacgccgc tcacgggcgt cgagaccggc ggcgtgccga ctctcgacct gcgtcgctcc
     1801 agcgagcccg ctccgccggc ggacccgggc gaggccacga gcgcccggcc cgtgagcgcc
     1861 ccgaagaagc cggacaccgg cgcatgagcg accccgctcc tccggacacc ggcaccggct
     1921 acggacggcc ccgggccacg ttcatgcgcg agctgaccct ggtgggcccg ggctcgccga
     1981 tgggcgagct gctgcgacgc tactggcatc ccgtcggcct ggtcggcgac gccggcgaga
     2041 cgccgaaggc cctgcgcgtg ctgggcgagg atctcgtgct cttccgcgac cggcgcgggc
     2101 gccccggcct cgtgaccgcg cgctgcgcgc atcgcggcac cacgctctac tacggccggg
     2161 tggaagagcg cggcatccgc tgctgctacc acggctggct cttcgacgtg gagggccgct
     2221 gcctcgagca gccctgcgag cccgacggcg gcccgcagcg cgagcggatg cgccagccct
     2281 ggtaccccgt ccaggagcgc tacggcctga tcttcgccta catgggcccg cccgcgcgca
     2341 agcccgtgct gccgcgctac gaagtgctcg agacgctcga cccgggcgag ttcctcgagg
     2401 ccgacgacag cagcgtgggc ggaggcgggc cggccgtgat cccgtgcaac tggctccagc
     2461 actacgagaa cctgatggac acgctccacg tcccgatcct gcacggctcc ttcagcggcc
     2521 cgcagttcac cgcgccgatg ggcctgatgc cggaggtgac ctgggcgctg accgacaagg
     2581 gcgtgaaggc gatctccgac cggcgcctgc ccgacggcac cgtgctgcac cggatcagcg
     2641 aggcggtgct gccgaccctc cgggtcatcc ccaatccgcg cgtggcgcag tacgcgcgcg
     2701 tggagaccat cggctgggtg ctgccgctcg acgatatgcg gttccgcatc tacacggcgg
     2761 ggcgggtgcg gaaggccggc gagatcgcga gcgtgcgctc gcgcctcaac ggcaagctct
     2821 gggaggagct gacgccggcc gagcatcgcg acttccccgg cgactacgag gcgcaggtcg
     2881 gccagggccc gatcgccctg cactccgagg agaacctggt gtcgagcgac cggggcgtgg
     2941 ccctggtgcg ccgccagctc aagcagcagc tcgacgcgct cgccgagggc cgcgacccga
     3001 tcggcgtgag cttcgacccc gccgcgccgc cggtgccgtt cgaggccggc aacttcctga
     3061 tcgagaggca gccccatgct tcgtgaccat ccgaccggcg actatcgcta catgcccggc
     3121 atcagcgcgt tctcgtgcgg cacggtggcc acaccgggcc acgagatcgt gcacgtgacg
     3181 ctcgccgcgc ccgtgccgtg gcgcgacggc ttcgcgcgca tcgagcggca cctccgcgag
     3241 gtgaagcgcc cgaagaccgc gctctgcggg atcgagctgc ggagccccgc gcccttcacc
     3301 ttcgacggct tcgccggctt caacgagggc taccgcaagc tcctggccga gtggggcatc
     3361 ctggtcggcg aggacaaccc gatcccgcgg accaacgtgg ccccggtcgc ggccgcgccg
     3421 caggagccgt cgctgtacgc cttctcctac acccagcccg gcgccacgcc cgagccgacc
     3481 ttcatcgtgg ccggcgcggg cgagctccgg gaccgcgcgc agggccccga gggcatcgtg
     3541 cgccacggcg acacctcgcc ggacgggatg cgcgagaagg cgcgcttcgt gatggccatc
     3601 atgcaggacc ggctgcgcac gctgggcggc gacttccgcc gcgtcaccac gatcgacatc
     3661 tacaccgccc agcccatcca cgccctcgtg ctcgacgaga tcctgaaggc cgcgggcccc
     3721 gccgccatcc acggcgtcca ctggtacccc agccgcccgc ccatccaggg cctcgactac
     3781 gagatggaca tgcgcggcgt cgcgcgcgag ctggtgctct agcctgcccc tcaccctgcc
     3841 ctctccccca gaggggagag ggagaattcg tctccatctt cgggaggaga gaagtcgtct
     3901 tcgcctgggg ggagggcttc tagctgctcc cctctcccca caggggagag gggagggtga
     3961 ggggctccga cggcacggaa ccgtagcgat gcacgaggtg cgagacggac tgctcccgca
     4021 cgtagtgccg cagctcgatc cggcccgcgg cgacgacggg cgcgtcgagc acgacgatcc
     4081 cggccgcgtg agccgccacg cgcagctcgc gcgagagcgc caccagcgcc cgcagccgct
     4141 cgacgccctc cccgccgagc cgcgcgatca gctcgccctc cgtctcggtc acggcgacga
     4201 ccgcgcgctc ctccccgagc cacgggccgg cctcacccgc cgacaggctc accgtcaccg
     4261 gcgccccgca cgtgcgcgcg gcgagcagtg ccagcgagag cgcgagccgc ccgtccgcgg
     4321 tcgccacctc gccccgcacc agcactcgcc ggcacggccg ataacggaac acgttgcgct
     4381 caccgagcag gcggatcggc tcgtgggccg gagcgaagtg ctcccgccac gcgcattcat
     4441 agccggcgcc ggcggcgcgc agcacggccg ccggcccctc ggcgggcggc gccaccgggc
     4501 gcgcgcggca gagctggagc acgtagttgg ggcccccggc cttggcgccc gggccgaccg
     4561 aggaggcctt ccagccgccg aaggactggc ggccgacgat ggcgccggtg atgtggcggt
     4621 tgacgtacag gttgccggcc tcgatgcgct cgagccagcg cgcgatctcg cggtcgtcca
     4681 gcgactggat cccgctggtc agcccgaacg gctgatcgtt ggcgagcgcg atcgcctcgt
     4741 cgaggtccgc ggcccgcatg agcccgagca ccgggccgaa gcactcggtc cgatggaaga
     4801 aggcgccccg gcgcacgccc agcttgatac ccggcgacca gagcggcgcc tcgcggctcg
     4861 gcgaacgcgg ctcgagcagc cactcctcgc ccggatccag cgtggtgagc gcgcgccgca
     4921 gcgcgggccc cggcggctgg gtcagcgggg tcacgcg
//
LOCUS       DASUFA010000382         8734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1593866, whole genome shotgun sequence.
ACCESSION   DASUFA010000382 DASUFA010000000
VERSION     DASUFA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593866"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFX87_13960"
     CDS             <1..302
                     /locus_tag="VFX87_13960"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEX4996083.1"
                     /translation="RIAAEGFADRVYADDGQLVTRRLGKDALITDPARAADQAVRMAM
                     EGKVRTITGKVIDARVETICIHGDSPGSDKIVAAVREGLVKAGAIVKPLRDWLPR"
     gene            791..1036
                     /locus_tag="VFX87_13965"
     CDS             791..1036
                     /locus_tag="VFX87_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996084.1"
                     /translation="MPSDSQIAHALYDAQSAQTQRSLDEILAMAQQSSGWTQVFKQLK
                     TEGLIEEQTLGHVVARWTNRTIRTRRPSLASVENISA"
     gene            complement(1054..2073)
                     /locus_tag="VFX87_13970"
     CDS             complement(1054..2073)
                     /locus_tag="VFX87_13970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4996085.1"
                     /translation="MKITFLCPHLRIAGGVRAILIHADRLARRGHDVTLVLPARRGLT
                     AWWRNRFQRAPEWMPTFGARLQWVPEWSAATLPEGDAVIATSWQSVEPVAAAPPSCGR
                     KFYFIQHYESLYHGEAARVDATYRVPLRKIVISTWLADIMAERFDTPAAVVVTPVDAT
                     LFRPVPVEDDGRLRVLMLFHEYPWKGVADGLEAFSRVRARHPELSLVGFGVKPPPRKL
                     PFAEFHENLPQERLAWLYSRCPIYLCPSWDEGLGMPSMEAMACGAALCTYDNGGCRDY
                     AIDGRTAVVSPRRDLDALAWGLTRLVEDPELRERIARQGQDFVGTQFDWERATARLEL
                     ILAAR"
     gene            complement(2070..3722)
                     /locus_tag="VFX87_13975"
     CDS             complement(2070..3722)
                     /locus_tag="VFX87_13975"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4996086.1"
                     /translation="MSELNGGHLVARTLKQAGVGHIFTLCGGHILPIYDGCITEDVRV
                     VDMRHEQACAHAADAYARLTRNIGVAVVTAGPGVTDAVTGIANAYSARSPVLLIGGAA
                     PLGLRGLGSLQEVEQVDLLRPITKGSWTVSETRQIPEVLTTAIRHAVTGRPGPVFVEI
                     PVDLLMTVVEDRLAPIPTAYAHRRPTAPDADSVQELAALLARAERPVIMAGSGVYWDD
                     AAKDLAALAERAGVPVFMNGAGRGSLPHDHPQAFAQARGFALGRADVALVLGTPLDFR
                     LGYGRPPSFAENVQVAMVDCDPVELGRNRPLAVGLASHIGLTLRALTDALPRDLAARV
                     QPWQAAVRRKETESQARLMTERLSDDVPISHYRLGHEIAAVVDAETTVVGDGGDVVGC
                     ASKIVPLSRPGQWLDPGPFGCLGVGPSFAIAAKLLRPSRRVLLIAGDGAFGLNGMEME
                     TAVRFGLPMTVVIGNDGGWGQIRNPQLSFFGAERAVGTSLPFTRFERMVEALGGTGIH
                     VTEPGQLRPALERALGSGEVYCVNVVLDPAAYRRTGQVSMAI"
     gene            complement(3710..4078)
                     /locus_tag="VFX87_13980"
     CDS             complement(3710..4078)
                     /locus_tag="VFX87_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4996087.1"
                     /translation="MKARLHHLQINVREASRALPFYRALFGYLEYGTVYDTDAMAGFS
                     GRGTDIWVGAAAPEHTGPGLHRKRAGLNHPAFRVDRREDVDRFRDEFMAPRKLAPLYG
                     TPREFPEYRPSMADSLVCQS"
     gene            complement(4075..4776)
                     /locus_tag="VFX87_13985"
     CDS             complement(4075..4776)
                     /locus_tag="VFX87_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4996088.1"
                     /translation="MKPRECKAEVAAVRAVTPEIMEVDLRVHEPADFTFEAGQWISVP
                     FGPKIVRAWSMVSPPTRKGGFTLSIDVAPGGIGSQWARGLKPGDPVQFKGPTGGFVFN
                     RADPRRAVFVAEEIGIVPIRSILAHLYETGYGRPTALIYWARNPGWLVYDADFRSLAR
                     RYPGFSYLPVLREPPATWRGEKGEPAEAVDRLVYGVDKLVVYVCGGGETINRVREVLV
                     SKGMDRKSVKWEKFW"
     gene            complement(4773..5693)
                     /locus_tag="VFX87_13990"
     CDS             complement(4773..5693)
                     /locus_tag="VFX87_13990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996089.1"
                     /translation="MKFGAFLSLHPPEEQFALARRCEELGLDSLWTGDHVSFHGPIYE
                     SLTLLAAYAGITKRIRLGSGVYLLALRHPAIAAKVTSTLDALSGGRLIFGVGVGGENP
                     KEFEACGIPHNERGARVTEGIDVVRTLWRDTHASFKGRFTQFEGVSIDPKPVQRPGPP
                     IWVGGRSDAALKRAGRQGDGWVSYVVQPERYTQSLAKVREAAEQAGRRLDGFVAAHLT
                     FITVGRDYESAERTWVRILSRRYAQDFGPLARKYGVIGTPEQCAEQMDRFTRAGCHYF
                     VMNPICDPEDERAQLEAIASEVIPRAPTAA"
     gene            complement(5695..7755)
                     /gene="rhaD"
                     /locus_tag="VFX87_13995"
     CDS             complement(5695..7755)
                     /gene="rhaD"
                     /locus_tag="VFX87_13995"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEX4996090.1"
                     /translation="MNSRWSDADAQGKNDLDLLVYASRLIGAETTLVVWGGGNTSVKR
                     DERDHRNRPTRVLRVKGSGSDLKSIQPRDFPGVRMDDVLALLERQDMGDQEMVDYLAH
                     ALQEPASPRPSIETLLHGFLPATAVIHTHADAVVSLTNNDRSRDVLREVYGDEVIPLA
                     YRRPGFLISREVAQTFEAHPTARALLLEKHGTICWGATVQAAYLATIELISRAEEAIA
                     HRARGRVRFGGVALASPLPEERRRVALAVAPHLRGLVGRDRRQVLFFDDAPDVLEFAG
                     AREAEALCRIGPATPDHTIFTKRLPCFAPVEDPADPESVRAGVSRAVERFVADYTAYF
                     EAHNRGGAKLTDPFPRVVVVGGLGLFAIGKDRRTAGIVNDIYHHTISVLGAATSFGGY
                     VSLTARDAFDVEYWPLELYKLSLAPPEKELARRIALVTGGASGIGRSAAQRLAAEGAH
                     VVVADLDAAGARKTAEEIVGAVGAGRAVGLGMDVTGEASVRAAFEAAVLTYGGVDIVV
                     SNAGTAHSAPVDRMELADWERSFAVNSTGHFLVAREGMRVLKAQGLGGAFVFVATKNV
                     MSPGKDFSAYSASKAAEAQLAKVLALEGGPHGIRSNIVNPDAVFRDSKLWSEDVRRER
                     ARAQGISVDDLEDFYRKRNILARPILPEDVAEAVLFLASDRSAKTTGCTITVDGGVKD
                     AFPR"
     gene            7850..8701
                     /locus_tag="VFX87_14000"
     CDS             7850..8701
                     /locus_tag="VFX87_14000"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX4996091.1"
                     /translation="MTAWTRRGFALGAALIFTGMGTCLGVPGPRAARADGIMLIQAGA
                     FWMGSDDDAPDERPLHRVFVRDFWLDRHKVTNREVALFLEARGLRSAEGLDYFDAGDP
                     DARIHRRDGRFTPDPGFEDHPAVEVSWYGARDYCLWRGKRLPTEAEWEKAARGTDRRR
                     HPWGDTPPGPEHAVFGRSYNATERGDQRPAGAGPTGVQDLLGNLREWTASEPRPYPYR
                     PDDGREAAGPRAARVVRGASHDDPPETLRVTIRRFYSYDGPRRGLARGHHHVGFRCAT
                     SEDLGGY"
BASE COUNT         1282 a         3219 c         3049 g         1184 t
ORIGIN      
        1 tccgcatcgc ggccgagggc ttcgcggacc gggtctacgc cgacgacggc cagctcgtga
       61 cccgcaggct cggcaaggac gcgctgatca cggatccggc cagggccgcc gaccaggccg
      121 tccgcatggc catggagggc aaggtgcgca cgatcaccgg caaggtgatc gacgccaggg
      181 tcgagaccat ctgcatccac ggcgacagcc ccggctcgga caagatcgtg gccgcggtcc
      241 gcgagggact ggtcaaggcc ggcgccatcg tgaagccgct cagagactgg ctgccccgct
      301 agcgccccga cgcagatcct gcgggggtcg gtcggaccgt ccgaaatcgg tacactgtat
      361 tccgttgctc gtcacccgcc tccggcggat ccgccgggtg gtgtcgcgta actctccatc
      421 ggaacgtgcc tttcccccct ccaaagaatg gcaccgagtt tgccctatgt tgggtcggcg
      481 caaccatggc ggactatgcg gacggcagca gcgtgaaggg cgtgagaccc ggcggatgcc
      541 ggcccggcga aatcaggtcg ggtgcgggca gccgtggaca ccgtctctcg gacccgcacg
      601 ggccccgcgg agccttcagg gagcgtcacg tcggccagat cgcgcgggct caccggctcc
      661 cgcatcacca gcgcggtctg gggcgatgag tgctcaggcg gaagccggga acgccatagg
      721 ggaggaaccc atgtcaacca ccacactgag actgctgacg ggatcgggtt accggcgcga
      781 gccgagcgcg atgccgtcag actcgcagat cgcgcatgcc ctgtatgacg cgcagagtgc
      841 tcagacccag aggagcctcg acgagatcct ggccatggcc cagcagagct ccggctggac
      901 gcaggtcttc aagcagctga agacggaagg cctgatcgag gagcagacgc tcggtcacgt
      961 ggtggctcgc tggacgaatc gcacgatccg tacccggcgg ccctcgctgg cgtcggtcga
     1021 gaacatctcc gcctaggcgt cccgcatcac gcgctaccgc gcggccagga tcagctccag
     1081 ccgcgcggta gcgcgttccc agtcgaactg agtccccacg aagtcctgcc cctgccgcgc
     1141 gatccgctcc cgtagctccg gatcctccac cagacgggtg agcccccagg ccagcgcgtc
     1201 cagatcgcgc cgcggcgaca ccaccgcggt gcggccgtcg atggcgtagt cgcgacaccc
     1261 cccgttgtca taggtgcaga gcgcggcccc gcaggccatc gcctccatcg agggcatgcc
     1321 gaggccctcg tcccacgacg ggcagaggta gatcgggcag cggctgtaga gccacgccag
     1381 gcgctcctgc ggcaggttct cgtggaactc ggcgaacggc agcttgcgcg gcggcggctt
     1441 cacgccgaag cccaccaggc tgagctcggg atgacgcgcc cgcacccggc tgaaggcctc
     1501 gaggccgtcg gccacgccct tccaggggta ttcgtggaac agcatcagca cgcggaggcg
     1561 cccgtcgtcc tcgaccggca ccgggcggaa gagcgtggcg tccaccgggg tgaccaccac
     1621 cgcggccggc gtgtcgaagc gctcggccat gatgtcggcg agccacgtcg agatcacgat
     1681 cttccgcagc gggacgcgat aggtcgcgtc cacccgcgcc gcctcgccgt ggtagaggct
     1741 ctcgtagtgc tggatgaagt agaacttccg gccacagctg ggcggggccg ccgccaccgg
     1801 ctccaccgac tgccacgacg tggcgatcac cgcgtcgccc tcgggcagcg tcgccgcgct
     1861 ccactccggc acccactgca gccgggcccc gaacgtcggc atccactcgg gcgcgcgctg
     1921 gaagcgattg cgccaccatg cggtcagccc acggcgggcc ggcaggacga gggtcacgtc
     1981 gtggccgcgc cgggccagcc ggtcggcgtg gatgaggatc gcccgcaccc cgcccgcgat
     2041 ccgcagatgc ggacagagga atgtgatctt catatcgcca tgctgacctg gccggtgcgc
     2101 cggtacgcgg ccgggtccag caccacgttg acgcagtaga cctcccccga gcccagcgcg
     2161 cgctccagcg ccggccggag ctggcccggc tcggtcacgt ggatcccggt gccgccgagg
     2221 gcctccacca tacgctcgaa ccgggtgaac ggcagcgagg tgcccaccgc gcgctccgcg
     2281 ccgaagaagg agagctgcgg gttgcggatc tgcccccacc ccccgtcatt gccgatcacc
     2341 accgtcatcg gcaggccgaa gcgcaccgcg gtctccatct ccatgccgtt gagcccgaag
     2401 gcgccgtcgc ccgcgatcag gagcacgcgc cgggacggcc gcagcagctt ggccgcgatc
     2461 gcgaacgagg gcccgacgcc caggcacccg aagggcccgg gatcgagcca ctggcccggc
     2521 cgcgagagcg gcacgatctt ggaggcgcag cccaccacgt ccccgccgtc gcccaccacc
     2581 gtggtctcgg cgtcgaccac cgccgcgatc tcgtggccca ggcggtagtg gctgatcggc
     2641 acgtcgtccg agagccgctc ggtcatgagc cgggcctgcg actcggtctc cttccgccgc
     2701 accgcggcct gccagggctg cacgcgcgcg gccaggtcgc gcggcagcgc gtcggtgagc
     2761 gcccgcagcg tgagcccgat gtggctggcg aggcccaccg ccagcgggcg gttacggccc
     2821 agctcgaccg ggtcgcagtc caccatggcc acctggacgt tctcggcgaa cgacggcggg
     2881 cggccgtagc ccagccggaa gtcgagcggc gtgcccagca cgagcgcgac gtcggcccgg
     2941 ccgagggcga agccgcgggc ctgcgcgaag gcctgggggt gatcgtgcgg gaggctgccg
     3001 cgccccgctc cgttcatgaa cacgggcacg cccgctcgct cggccagcgc cgcgaggtcc
     3061 ttcgccgcgt cgtcccagta gacaccgctg cccgccatga tcacgggccg ctccgcgcgg
     3121 gccaggagcg cggccagctc ctggacggag tcggcgtcgg gcgcggtggg gcgccggtgc
     3181 gcataggcgg tggggatcgg ggccaggcgg tcctccacca cggtcatcag caggtccacc
     3241 gggatctcga cgaagaccgg gcccggccgc ccggtgaccg cgtgccggat cgcggtggtc
     3301 agcacctcgg ggatctggcg ggtctcgctc accgtccacg agcccttcgt gatgggccgc
     3361 agcaggtcca cctgctccac ttcttgcagc gagccgagcc cgcgcaggcc gagcggcgcg
     3421 gccccgccga tcaggagcac cgggctgcgc gcggagtacg cgttggcgat gccggtgacc
     3481 gcgtcggtga cgcccgggcc cgcggtcacc accgccacgc cgatattgcg ggtgagccgc
     3541 gcgtaggcgt cggcggcgtg cgcgcaggcc tgctcgtggc gcatgtccac gacccgcacg
     3601 tcttccgtga tgcagccgtc gtagataggc aggatgtggc cgccgcacag cgtgaagatg
     3661 tggccgaccc ccgcctgctt gagcgtgcgc gcgacgaggt gccccccgtt cagctctgac
     3721 ataccaagct atccgccatg ctcggccggt actccgggaa ctcgcgcggg gtgccgtaga
     3781 gcggggcgag cttccgcggg gccatgaact cgtcccggaa gcgatccacg tcctcccgac
     3841 ggtccacgcg gaaggccggg tggttgagcc cggcccgctt gcggtgaagg cccggcccgg
     3901 tgtgctccgg ggcggcggcg cccacccaga tgtcggtccc gcggccggag aagccggcca
     3961 tcgcatcggt gtcgtagacg gtcccgtatt ccagatagcc gaagagcgcc cggtagaacg
     4021 gcagggcccg cgacgcctcc cgcacgttga tctggaggtg atggagccgg gccttcacca
     4081 gaacttctcc cacttgacgc tcttgcggtc catgcccttg ctgaccagga cctcgcggac
     4141 cctgttgatc gtctccccgc ccccgcagac atagaccacc agcttgtcca cgccgtacac
     4201 cagccgatcc accgcctccg ccggctcgcc cttctcgccg cgccaggtcg cgggcggctc
     4261 gcgcagcacc ggcaggtacg agaagcccgg gtagcggcgc gcgagcgagc ggaagtcggc
     4321 gtcgtagacg agccagccgg ggttgcgcgc ccagtagatc agcgcggtgg gccggccgta
     4381 gcccgtctcg tagaggtgcg ccaggatgga ccggatcgga acgatcccga tctcctcggc
     4441 cacgaagacg gcgcggcgcg ggtcggcgcg attgaagacg aagccgccgg tcggcccctt
     4501 gaactgcacc ggatcccccg gcttgagccc gcgcgcccac tgcgagccga tcccgcccgg
     4561 ggccacgtcg atcgagagcg tgaagccccc cttgcgggtg ggcggcgaga ccatgctcca
     4621 cgcgcgcacg atcttgggac cgaagggcac cgagatccac tgccccgcct cgaaggtgaa
     4681 gtcggcgggc tcgtgcaccc gcagatccac ctccatgatc tcaggcgtca ccgcgcgcac
     4741 cgcggcgacc tcggccttgc actcgcgcgg cttcatgccg ccgtcggcgc gcggggaatg
     4801 acctcgctcg cgatggcctc gagctgcgcc cgctcgtcct ccggatcgca gatggggttc
     4861 atcacgaagt agtggcagcc ggcgcgcgtg aagcggtcca tctgctcggc gcactgctcg
     4921 ggcgtgccga tcaccccgta cttgcgggcg agcggcccga agtcctgcgc gtagcgccgc
     4981 gagaggatgc gtacccaggt ccgctcggcg ctctcgtagt cgcggcccac ggtgatgaac
     5041 gtgagatgcg cggccacgaa gccgtccagg cggcggcccg cctgctcggc ggcctcgcgg
     5101 accttggcca ggctctgggt atagcgctcg ggctggacga cgtaggacac ccagccgtcg
     5161 ccctgacgcc cggcccgctt cagcgccgcg tcggaccggc ctcccaccca gatcggcggc
     5221 cccggccgct gcaccggctt cggatcgatg ctcacgccct cgaactgggt gaagcgcccc
     5281 ttgaacgagg cgtgcgtgtc tcgccagagc gtccgcacca cgtcgatccc ctcggtgacc
     5341 cgggccccac gctcgttgtg gggaatcccg caggcctcga actccttcgg gttctcgccg
     5401 cccaccccga ccccgaagat gagccggccg ccggagagcg cgtcgagcgt ggaggtgacc
     5461 ttggccgcga tggcgggatg gcggagcgcc agcaggtaca cgccgctgcc cagcctgatg
     5521 cgcttggtga tgcccgcgta ggcggccagc agcgtcagcg actcgtagat cgggccgtgg
     5581 aaggacacgt ggtcgcccgt ccacagcgag tcgagcccca gctcctcgca gcgccgggcg
     5641 agcgcgaact gctcctcggg cggatgcagc gagagaaacg ccccgaactt caccttagcg
     5701 ggggaaggca tccttcaccc cgccgtccac cgtgatcgtg cagcccgtgg tcttggccga
     5761 tcggtccgag gccaggaaga gcaccgcctc cgcgacgtcc tccggcagga tcggccgggc
     5821 gaggatattg cgcttgcggt agaagtcctc gagatcgtcc accgagatgc cctgcgcccg
     5881 cgcgcgctcg cgccggacgt cctccgacca gagcttggag tcgcggaaga cggcgtccgg
     5941 gttgacgatg ttggaccgga tgccgtgggg cccgccttcg agcgccagca ccttggcgag
     6001 ctgggcctcg gcggccttgg acgcggagta ggccgagaag tccttgccgg gcgacatgac
     6061 gttcttggtg gccacgaaga cgaaggcgcc gcccagcccc tgggccttga gcacgcgcat
     6121 gccttcgcgc gccaccagga agtggccggt ggagttcacc gcgaaggagc gctcccagtc
     6181 ggcgagctcc atgcggtcca ccggcgccga gtgcgcggtt cccgcgttgg agaccacgat
     6241 gtccacaccg ccgtaggtca gcaccgccgc ctcgaaggcg gcgcgcaccg aagcctcgcc
     6301 ggtcacgtcc atgccgagcc ccaccgcccg ccccgcgccc acggcgccca cgatctcctc
     6361 ggccgtcttg cgcgcgcccg ccgcgtccag atcggccacc accacgtgcg cgccctcggc
     6421 ggccagccgc tgggccgccg agcgcccgat gccgctggcg cccccggtga ccagcgcgat
     6481 gcggcgcgcc agctccttct cgggcggggc cagcgagagc ttgtagagct cgagcggcca
     6541 gtactcgacg tcgaaggcgt cccgggccgt gagcgacacg tagccgccga acgaggtggc
     6601 cgcgcccagg acggagatcg tgtggtggta gatgtcgttc acgatgcccg cggtccgccg
     6661 gtccttgccg atggcgaaga ggccgagccc gcccaccacg accacgcggg gaaacgggtc
     6721 cgtcagcttg gccccgccgc ggttgtgggc ctcgaagtac gcggtgtagt cggccacgaa
     6781 gcgctccacc gcccggctga cccccgcgcg gaccgactcg ggatccgccg ggtcctcgac
     6841 cggcgcgaag catggcagcc gcttggtgaa gatcgtgtgg tccggcgtgg cgggcccgat
     6901 ccggcagagg gcctccgcct cgcgcgcgcc cgcgaactcc agcacgtccg gcgcgtcgtc
     6961 gaagaacagc acctggcgcc ggtcgcgccc caccagcccg cgcagatgcg gggccaccgc
     7021 gagggccacc cgccgccgct cctccgggag cgggctcgcg agcgcgacgc cgccgaagcg
     7081 tacccgtccc cgggcgcgat gggcgatcgc ctcctcggcg cggctgatca gctcgatcgt
     7141 ggcgaggtag gccgcctgca cggtggcccc ccagcagatc gtcccgtgct tctcgagcag
     7201 cagcgcgcgc gccgtcggat gcgcctcgaa ggtctgcgcg acctcccggg agatgaggaa
     7261 tccggggcgc cggtacgcga gcgggatgac ctcgtcaccg tagacctcgc gcaggacgtc
     7321 gcggctgcgg tcgttgttgg tgagcgagac caccgcgtcc gcgtgggtgt ggatcaccgc
     7381 cgtcgcgggc aggaagccgt gcagcaaggt ctcgatggac gggcgcgggc tcgcgggctc
     7441 ctgcagcgcg tgggcgagat agtccaccat ctcctgatcg cccatgtcct gccgctcgag
     7501 cagggccagc acgtcgtcca tgcgcacgcc ggggaaatcc ctcggctgga tgctcttgag
     7561 gtcggagccg ctgcccttca cgcgcagcac gcgcgtcggc cggttccggt gatcccgctc
     7621 gtcccgcttc acggacgtgt tgccgccacc ccacaccacc agcgtggtct cggcgccgat
     7681 cagccgggac gcgtagacga ggagatcgag atcgttcttg ccctgcgcgt cggcatcgct
     7741 ccatcgggag ttcatggcgc tctcacgata cccgaatctt cccgccaaat cacctggccc
     7801 aaatcacctg tcgattgaca cccctgggcg cggctcctat cgtatgcacg tgaccgcgtg
     7861 gacgcggcgg ggcttcgcgc tgggcgcggc gctgatcttc acgggcatgg gcacctgcct
     7921 cggcgttccc gggccgcgcg ccgcccgcgc cgacgggatc atgctcatcc aggccggcgc
     7981 gttctggatg gggagcgacg acgacgcccc cgacgagcga ccactccacc gcgtcttcgt
     8041 ccgcgacttc tggctggacc gtcacaaggt cacgaaccgg gaggtcgcgc tcttcctcga
     8101 ggcgcgcggg ctccggagcg ccgagggtct ggactacttc gacgcgggcg accccgacgc
     8161 ccgcatccac cgccgcgacg gccgcttcac ccccgacccc ggcttcgagg atcatccggc
     8221 cgtggaggtg tcctggtacg gcgcccgcga ctactgcctc tggcggggca agcgactgcc
     8281 caccgaggcc gagtgggaga aggcggcccg gggaacggac cggcgccgtc acccctgggg
     8341 ggacactccg cccgggcccg agcacgcggt gttcgggcgc tcctacaacg ccaccgagcg
     8401 cggcgaccag cgcccggccg gcgccgggcc caccggggtc caggacctcc tcggcaacct
     8461 gcgggagtgg acggcctccg agccccgccc ctacccctac cgtccggacg acggccgcga
     8521 ggcggcgggg ccccgtgccg cgcgcgtggt acggggcgcc agccacgacg atcccccgga
     8581 gacgttgcgg gtgacgatcc ggcgcttcta ctcgtatgat ggaccccgcc gcggcctggc
     8641 ccgtggccat caccacgtcg ggtttcgctg tgcgacttcg gaggacctgg gaggatattg
     8701 acgcgatgaa aggcctgatg atggattacc ccct
//
LOCUS       DASUFA010000383         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1681908, whole genome shotgun sequence.
ACCESSION   DASUFA010000383 DASUFA010000000
VERSION     DASUFA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681908"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFX87_14005"
     CDS             complement(<1..785)
                     /locus_tag="VFX87_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4996092.1"
                     /translation="MADRGIDVVRAHLAKLPPSDSLTTAERRAQYERAEKVFPTPPDV
                     KVERVNAPAAPAEWLRPPSAEPGRVLLYLHGGGYVIGSPRSHRHLAAAIAGAAGASAL
                     LLDYRLAPEHPYPAAVEDATAAYRWLLDQAIAPERIVIAGDSAGGGLTVATLLALREA
                     RVPLPAGGVCISPWVDLTCGGASYATKADADPIVRRAGVEQMAQAYLGATPPRTPLAS
                     PLFADLRGLPPLLIHVGTDEVLLDDAVQLTERARAAGVDTTLD"
     gene            909..1439
                     /locus_tag="VFX87_14010"
     CDS             909..1439
                     /locus_tag="VFX87_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4996093.1"
                     /translation="MIPLVDAHHDIWRQADLAWLQGPPQPRIFGEYGAIRRDYPIEEY
                     MGEARAAGVERSVYVQTNWPPGREVEEVAWVQSVADRHGFPHAIVGHADLAAASLETT
                     RACTVDRWGPVIRETVELFGPERCMFGSNFPVEKLWTTYDEVVRVTAEGLAGLSAAEQ
                     RAVLHDTADRVYRLGS"
     gene            1481..2299
                     /locus_tag="VFX87_14015"
     CDS             1481..2299
                     /locus_tag="VFX87_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4996094.1"
                     /translation="MTAPEFLYGTAWKEDRTPALVELALRTGFRGVDTANQRRHYFEA
                     GVGQGLAAAYRAGIVTRAELFLQTKYTYQPGQDHRLPYDPDADLTTQVAQSMASSLEH
                     LGTDHVDSYVLHGPASGHGWTEYDAEVWGAMARERDAGRTRLLGVSNVSLRHLQQMVA
                     TGAEMPAFVQNRCFAVRGWDRDVRAFCAQRRIVYQGFSLLTANPDVLRHPVVTGIAAR
                     SQAVPAQVVFRFALAVGMLPLTGTSSPEHMKQDLAARALALSADEVRAIEALAG"
     gene            2307..3233
                     /locus_tag="VFX87_14020"
     CDS             2307..3233
                     /locus_tag="VFX87_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996095.1"
                     /translation="MRRLTVLMPFQTPFYAPLPAGVALGHFRDEGLSVAAVAATNYGK
                     GTMPALLDGDIEISLGGLMRSFELADRSGPIVVHFAEVCSRNGFFLLSRKPRPTFAWA
                     DLIGRTVLSFAEAPTPWQCMLTVLRRHGVDAARVRIERTRPALDAVAAFRAGYGDFLE
                     QPQPVVEQLLAEGAAHLVASMGEATGPVPFTSYMTTPAFLAREPQVVRAFTRALYRTQ
                     RWLARAEPPEIAAVIAPAFPETDTAILEHAVARYHRQGTWARDPLLRREGYDSLEEIL
                     LEGGFIARRARYEDLVDTEIARHVMAESPATA"
     gene            3325..3795
                     /locus_tag="VFX87_14025"
     CDS             3325..3795
                     /locus_tag="VFX87_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4996096.1"
                     /translation="MTSVTVNGRRYNVNLPDDTPLLWALRDELGLTGTKYGCGMALCG
                     ACTVHVDGQPVRACQTPISAVSGSKVTTIEAISTDRLGRALQAAWVDMGVPQCGYCQA
                     GQIMSATALLRRTPKPSDAQIDEAMSGNICRCGTYVRVRAAIKQAAAGQSGAAR"
     gene            3792..>4759
                     /locus_tag="VFX87_14030"
     CDS             3792..>4759
                     /locus_tag="VFX87_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4996097.1"
                     /translation="MSARRAEGVTDRRPLVLANLSRRGFLQGMTAAGGFVLAAGFSSA
                     ARAADPPKYGADGMPNGWVDDPLVFVAIAPDGIVSITCHRSEMGQGVRTGMPMIVADE
                     LEADWKRVRVVQAPGDEKRFGNQDTDGSRSTRHFFTPMRRCGAAARTMLEAAAAKRWG
                     VPVTEVEARNHEVVHAKSGRRAGYGSLAKEAAQQPVPARDTVRLKDPSKFRYIGKGQL
                     KLVDAPAMVSGKAQYGIDTRLPGMLYAVVARPPVYGGKVVSVDAAEALKVAGVLKVVE
                     IDPSPAPAQFNPVGGIAVVGRNTWAAIQGRKALKVVWNDGPNGSYDS"
BASE COUNT          686 a         1629 c         1769 g          675 t
ORIGIN      
        1 atgtccagag tggtgtccac gccggcggcc cgggctcgct cggtgagctg aaccgcgtcg
       61 tcgagcagca cctcgtcggt gcccacgtgg atcagcagcg gcgggaggcc gcgcaggtcg
      121 gcgaagagcg gtgaggccag cggggtccgg ggcggcgtgg cacccagata ggcctgcgcc
      181 atctgctcga ccccggcgcg gcgcacgatc ggatccgcgt ccgccttcgt cgcgtagctg
      241 gcgccgccgc aggtgagatc cacccagggc gagatgcaga ccccgcccgc gggcagcggc
      301 acgcgcgcct cgcgcagggc cagcagcgtg gccaccgtga ggccgccgcc cgcggagtcg
      361 cccgcgatca cgatgcgctc gggggcgatg gcctggtcca gcagccagcg atacgccgcg
      421 gtcgcgtcct ccaccgcggc cgggtaggga tgctcgggcg cgaggcggta gtcgagcagg
      481 agcgcgctgg ctcccgcggc gcccgcgatg gcggcggcca ggtgacggtg cgagcgcggg
      541 gaaccgatga cgtaaccgcc gccgtggaga tagagcagca cgcggccggg ctcggcggag
      601 ggcggccgca gccactccgc cggggcggcg ggggcgttca cgcgctccac cttgacgtcg
      661 ggtggcgtgg ggaagacctt ctcggcgcgc tcgtactggg cccggcgctc ggcggtggtc
      721 agcgagtcgg acgggggcag cttcgcgagg tgcgcgcgaa cgacgtcgat gccgcggtca
      781 gccatggctc tcctccatga ggagggattc tgcgccggga cgagggtggt tggcaagcga
      841 ctgagcgcct ggacccgctg catggtgtac tctccacccc cgaggagaga tcccccgagg
      901 tgacgttcat gatcccgctc gttgacgccc atcacgacat ctggcgccag gccgacctgg
      961 cctggctgca gggcccgccg cagccgcgga tcttcgggga gtacggcgcg atccgccgtg
     1021 actacccgat cgaggagtac atgggcgaag cgcgcgccgc cggcgtggag cggtcggtct
     1081 acgtgcagac caattggcca cccgggcggg aggtggagga ggtcgcgtgg gtgcagtcgg
     1141 tggccgatcg ccacgggttc ccgcacgcga tcgtgggcca cgccgatctg gccgcggcga
     1201 gcctcgagac cacgcgtgcc tgcacggtcg atcgctgggg gccggtgatc cgggagaccg
     1261 tcgagctctt cggcccggag cgctgcatgt tcgggagcaa cttcccggtc gagaagctgt
     1321 ggacgaccta cgatgaggtg gtccgcgtca ccgccgaggg tctggccggg ctctcggccg
     1381 ccgagcagcg tgcggtgctc cacgacaccg ccgaccgcgt gtatcggctc ggatcgtgag
     1441 cctcgatccg cggaccgggc actcgtggca ggatggtgcg gtgaccgcgc cggaattcct
     1501 gtacggcacc gcgtggaagg aagaccgcac tcccgccctc gtggagctgg ccctgcgcac
     1561 ggggttccgg ggcgtcgaca ccgccaacca gcggcggcac tacttcgagg cgggggtggg
     1621 gcaggggctg gccgctgcct atcgcgcggg catcgtcacg cgcgcggagc tctttctgca
     1681 aacgaagtac acgtatcagc ccgggcagga ccaccggctg ccctacgatc ccgatgccga
     1741 tctcaccacc caggtggccc agtccatggc cagctcgctc gagcatctgg gcacggacca
     1801 cgtggatagc tacgtgctgc acggacccgc ctccggccac ggctggacgg agtacgacgc
     1861 ggaggtctgg ggcgcgatgg cgcgcgagcg ggacgcgggc cgtacgcggc tgctcggggt
     1921 cagcaatgtc tcgctccggc acctgcagca gatggtggcc accggcgccg agatgcctgc
     1981 cttcgtgcag aaccgctgct tcgcggtccg cggctgggat cgcgacgtgc gggccttttg
     2041 cgcgcagcgc cgcatcgtct accagggctt ctcgctgctc accgccaacc cggacgtgct
     2101 tcgccacccg gtggtgaccg gcatcgccgc gcgcagtcag gcggtgcccg cgcaggtagt
     2161 gttccgcttc gcgctcgcgg tcgggatgct tccgctgacc ggcacgtcga gcccggagca
     2221 catgaagcag gacctggccg cccgcgccct cgcgctctcc gccgacgagg tgcgagcgat
     2281 cgaagcgctc gcgggctagg cgggcgatgc gtcgcctcac cgtgctcatg ccctttcaga
     2341 cacccttcta tgcgccgctg cccgccggcg tcgcgctcgg ccatttccgg gacgaaggcc
     2401 tcagcgtggc cgccgtagcg gcgaccaact acggtaaagg cacgatgccc gcgctactcg
     2461 acggtgacat cgagatcagc ctgggcgggc tgatgcggag cttcgagctg gcggatcgct
     2521 cgggaccgat cgtggtgcac ttcgcggagg tctgtagccg gaacggtttc ttcctcctga
     2581 gccggaagcc ccgacccacg ttcgcctggg cggacctgat cggtcggacc gttctgtcgt
     2641 tcgcggaggc cccgacgccg tggcagtgca tgctgaccgt gctgcgcagg cacggcgtgg
     2701 atgcggcccg ggtgcggatc gagcggacgc gaccggcact cgatgcggtc gccgcattcc
     2761 gcgccgggta cggcgacttc ctcgagcagc cgcagccggt cgtggagcag ctcctcgccg
     2821 agggcgcggc ccacctggtc gcctcgatgg gcgaggccac ggggccggtg cccttcacgt
     2881 cgtacatgac cacgccggcc ttcctggcgc gcgagcctca ggtggtgcgc gccttcaccc
     2941 gcgcgctgta ccgcacgcag cgctggctcg cccgcgccga gccaccggag atcgcggcgg
     3001 tgatcgcgcc ggcgttcccc gagacggaca ctgcgatcct cgagcacgca gtggcccggt
     3061 atcaccggca gggaacctgg gcgcgggatc cactgctgcg ccgcgagggc tacgactctc
     3121 tcgaggagat cctgctcgag ggcggtttca tcgcgcggcg agcgcgctac gaggatctgg
     3181 tggacacgga gatcgcccgg cacgtgatgg cggagtcgcc cgccaccgca tagtcgttga
     3241 caggcccaag gccgcttgct atccggccca gtgacgtcgg gtacacgcga tagggcttgt
     3301 ctcaatgacg agaggagaat cgcgatgacc tcagtcaccg tcaacggccg ccggtacaac
     3361 gtcaacctcc ccgacgatac cccgctgctg tgggccctgc gcgacgagct cgggctcacc
     3421 ggtaccaagt acggctgcgg catggcgctg tgcggcgcct gcaccgtgca cgtggacggg
     3481 cagccggtgc gcgcctgcca gactccgatc tcggcggtga gcggtagcaa ggtcaccacc
     3541 atcgaggcca tctccaccga ccggctcggc cgggcgctgc aggcggcctg ggtggacatg
     3601 ggcgtgccgc agtgcggcta ctgtcaggcc gggcagatca tgtcggcgac cgcgctactc
     3661 aggcgcacgc cgaagccgag cgacgcccag atcgacgagg ccatgagcgg caatatctgc
     3721 cgctgcggga cctatgtgcg ggtgcgggcc gcgatcaagc aggcggcggc cggacagtcg
     3781 ggagcggcgc gatgagcgcc cggcgggcgg aaggcgtgac ggaccggcgc cctctcgtgt
     3841 tggccaacct gagccggcgg ggcttcctcc agggcatgac cgcggcgggc ggcttcgtcc
     3901 tcgcggccgg gttctcctcg gcggcgcggg ccgccgatcc gcccaagtac ggcgccgacg
     3961 gcatgcccaa tggctgggtg gacgatccgc tggtcttcgt ggccatcgcg cccgatggca
     4021 tcgtcagcat cacgtgccac cgctccgaga tgggccaggg cgtgcggacc ggcatgccca
     4081 tgatcgtggc cgacgagctg gaggccgact ggaagcgggt gcgcgtggta caggccccgg
     4141 gcgacgagaa gcgcttcggc aaccaggaca ccgacggctc gcgcagcacg cggcatttct
     4201 tcactccgat gcgccgttgc ggcgcagcgg ctcgcaccat gctcgaggct gcggcggcca
     4261 agcgctgggg cgtgccggtg actgaggtgg aggcccggaa ccacgaggtc gtgcacgcca
     4321 agagcgggcg gcgcgccggc tacggctcgc tagccaagga agcggcccag cagccggtcc
     4381 cggctcggga taccgtgcgc ctcaaggacc cgtccaagtt ccgctacatc ggcaagggcc
     4441 agctgaagct cgtggacgcc ccggccatgg tgagcgggaa ggcgcagtac ggcatcgaca
     4501 cgcggctgcc cggcatgctc tacgcggtcg tggcccggcc gcccgtctac gggggcaagg
     4561 tcgtcagcgt ggacgcggcc gaggccctca aggtggccgg ggtgctgaag gtggtggaga
     4621 tcgatcccag ccccgccccg gcccagttca acccggtggg gggcatcgcg gtggtggggc
     4681 gcaatacctg ggccgcgatt caaggtcgca aggcgttgaa ggtcgtgtgg aacgacggcc
     4741 cgaacggtag ctacgattc
//
LOCUS       DASUFA010000384         6916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_776837, whole genome shotgun sequence.
ACCESSION   DASUFA010000384 DASUFA010000000
VERSION     DASUFA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776837"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFX87_14035"
     CDS             complement(<1..830)
                     /locus_tag="VFX87_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996098.1"
                     /translation="MRRVCFVTCRTWPEISESDRLVQRALQARGAIVEPRAWNDPGAN
                     WDGFDAIVLRSNWDYHFEPDAFLGWLDRLERAGARIWNPPALVRWNLSKAYLLELARA
                     GVPTVPTVILADETRVGLEAVMAGRHWSRVVMKPGISASAHDTRLVSAATMDEAVAAL
                     VSGAIRRPVLVQPFVEEIQTRGEWSLVFIDTEMTHAVLKRPAAGEFRVQPRLGGTVEA
                     LEPPDAVGAVGRAALAALPVAPLYARIDGVETAAGFRIMEVEINEPGLFFPHAPAAAA
                     R"
     gene            complement(845..1726)
                     /locus_tag="VFX87_14040"
     CDS             complement(845..1726)
                     /locus_tag="VFX87_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX4996099.1"
                     /translation="MTVRLLSLALVLLALAGCGPAAPPAKPLVVATIYPLWEFARQVA
                     GDRAEVVSLVPAGVEPHDWEPAPRDVSQIQRAAVFVHTGTGLDGWAEKLLADLTGRRG
                     VVVNARGGLGLLTVGGVTDPHVWLDPTLARVQVLAIAGGLELADPAGHAVYAENVKAF
                     IARLDALDLEFAAGLADCARREIVTSHAAYAYLARRYRLTQVPVMGLAPEAEPSPADL
                     AAIVRTARRLKVTHVFYETLVSPRLAETLSREIGATPLPLNPIEGMSPAEAAAGTSYL
                     ELMRANLANLRTALGCR"
     gene            complement(1723..2547)
                     /locus_tag="VFX87_14045"
     CDS             complement(1723..2547)
                     /locus_tag="VFX87_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX4996100.1"
                     /translation="MTADLLALMWMPFLMCLVLTGIHAYLGFHVIAREVVFVDIALAQ
                     IAALGATAAFLLGYELATWQSYAAGLGFTILGALVLALTRSRRGLVSHEAVIGVVYAV
                     SAAGAVLLADRAPHGAEHLRGMLVGSLLSVRGAEVLKVAALYALIGVFHWLCRRPFFL
                     ISTDPDRAYRDGWRVRAWDFLFYASFGLVVTSSVRIAGVLLVFSYLIVPALAGILLAQ
                     RIGARLAVGWAFGAGVSVVAMLASAVLDLPTGATVVCAFGLSLLALGLVLRVRRVA"
     gene            complement(2554..3495)
                     /locus_tag="VFX87_14050"
     CDS             complement(2554..3495)
                     /locus_tag="VFX87_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX4996101.1"
                     /translation="MRIPIRAAAAAILTLSLGALGAPGLVSGDAAAADKLRVVTTTTD
                     LKALTEAVGGDLVEVEALARGNQNAHDLEVRPSLMVKVRRADLLVINGLELDQWAEVV
                     VQGANNPKVLPGTPGRVDASEGLPLLEVPTTRVDRSLGDVHPVGNPHYTMDPGMAPQI
                     TANVLNGLARLQPQSRPAFEQNRAAFLASLDQAMGRWTAALAPFKGAKVVQYHPLFVY
                     LLTRFGLVKGGAIEDRPGIPATPGHLARLIQDMKQERIKLVVVEPWNDVKLAERVAQE
                     AGAKVRVLAPSVGAVKEARSYLETVDYNVRTLAEGLR"
     gene            complement(3510..4928)
                     /locus_tag="VFX87_14055"
     CDS             complement(3510..4928)
                     /locus_tag="VFX87_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996102.1"
                     /translation="MRSLVGIGLLSASVLMTAPAAAQDVEALREELQQMRKQFETMKE
                     GYEKAIDRLGERIQQLETRPQPAVAAPAPAPAPSVAQAPAPAAPAIAVTPLDLARPRQ
                     PFGLYGNRGPGQLLFDMGIAGDFIGNFTQRNVDKANAGTFIGQENRFFPREVELALFG
                     QIDPYARAEVRIEAGEEDAGEIALHLAEAHLTLLALPFNTQLKMGQMRNRFGLLNPIH
                     EHDRPFIDTPNALRWFLGAEGLVERGAELTWVAPLPFFLEGLVGVFNGDNEDAFGRGK
                     LNEPLLTGRLRTFLELGDWSAIQLGVSVANGETPDRRRNTLLGFDAKYKYTPEGWQQP
                     LLTLLGELIYQERRINVLGEDTDGDGVQDTPDETRRFTRLGWYAGGEVRPFRTGWLKG
                     VGAGFRYDWTQYPVMRGREWAVGPFLSFSPSEFLRFRLGYKHTERATRDGFTDNESSA
                     RKVDEILFQATFILGAHPAHPF"
     gene            complement(5016..5324)
                     /locus_tag="VFX87_14060"
     CDS             complement(5016..5324)
                     /locus_tag="VFX87_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996103.1"
                     /translation="MRNLRPLGSLRLAAAALLLALVAVTLPLKASYPRHVHAASTAGV
                     YNEDHVLASLEFLGGDGPLPDLPAAVSLALITAACALPPRSRPGAPALRLAGSRAPPA
                     "
     gene            complement(5367..5786)
                     /locus_tag="VFX87_14065"
     CDS             complement(5367..5786)
                     /locus_tag="VFX87_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4996104.1"
                     /translation="MTRRSALVRAVVWAITLALTRAALAGHGGVPTVALTEPDYLKWL
                     LDSRQPVVVVDLRSPGDFRRGHLPGAVSVPLAELDRRFAEIPTQPMVVLYCRCPLEEA
                     ATAYSFLETKGYTNHVVLQEGYDGWLKRRYPLTRSPD"
     gene            complement(5783..6154)
                     /locus_tag="VFX87_14070"
     CDS             complement(5783..6154)
                     /locus_tag="VFX87_14070"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX4996105.1"
                     /translation="MRLWRVVLLVNLALGVGLLCGYLGWGREAARLGQELERTRLQQA
                     TAGSRRAWQARGVVRAVLPELNVVVLTHEDLAGYMGSMTMGFRVHDPKLYEGLDIGDT
                     VIFTLTGAPPNVEITAIAKQP"
     gene            complement(6161..>6916)
                     /locus_tag="VFX87_14075"
     CDS             complement(6161..>6916)
                     /locus_tag="VFX87_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4996106.1"
                     /translation="GDGPGGAGLPRLPADLTAAHAADHTAGDMFWWLSHGIPAGGMPP
                     FAAALGEEERWDLINFIRALAAGERARQMTAVVQPNRPWLVAPDFTIAVGPAPPRGLK
                     ELRERWMVHLVFFTLPDSRARLVQLAESYNTLQATGTEVIAIPLDDGAHIIRRLGGRP
                     PVLFPVITDGAAEIAATYGLLGRGLGPRELSPRAAPLLHVEFLLDRQGYVRARWLPGA
                     PGPGWETLQTLVDQILLLDKETPAGPPPDEHVH"
BASE COUNT         1029 a         2415 c         2587 g          885 t
ORIGIN      
        1 cgcgcggcgg ccgcgggcgc gtgggggaag aagagaccgg gctcgttgat ctctacctcc
       61 atgatccgga agccggccgc ggtttcgacc ccgtcgatcc gcgcgtagag cggggccacc
      121 gggagcgcgg ccagcgccgc gcgcccgacc gccccgaccg cgtcgggcgg ctcgagcgcc
      181 tccacggtgc cgccgaggcg gggctgcacc cggaactcgc ctgcggccgg ccgcttgagc
      241 accgcatggg tcatctcagt gtcgatgaag accagcgacc actcgccccg ggtctggatc
      301 tcctcgacga agggctggac caggaccggg cggcggatgg ccccggacac gagcgcggcc
      361 accgcctcgt ccatggtggc ggcgctcacg agccgcgtgt cgtgcgcgga ggccgagatc
      421 cccggcttca tcacgacccg gctccagtgg cggccggcca tcaccgcttc gagccccacc
      481 cgggtctcgt ccgccaggat gacggtgggc acggtcggca ccccggcccg ggcgagctcg
      541 agcaggtacg ccttgctcag gttccagcgg acgagggcgg gggggttcca gatgcgcgcg
      601 ccggcccgct cgagacgatc gagccagccg aggaaggcgt ccggctcgaa gtggtagtcc
      661 cagttcgagc ggagcacgat cgcgtcgaag ccgtcccagt tcgcgccggg gtcgttccac
      721 gcgcgcggct ccacgatggc gccgcgggcc tggagcgccc gctggaccag ccggtcgctc
      781 tccgagatct cgggccaggt ccggcacgtg acgaagcaga ccctccgcac agacggcagc
      841 ggggctagcg gcagccaagc gcggtgcgaa gattcgccag gttcgcgcgc atcagctcca
      901 ggtaactcgt gcccgctgcg gcctcggccg ggctcatgcc ctcgatggga ttcagcggca
      961 gcggggtcgc cccgatctcg cggctcaagg tctcggccag gcgcgggctg accagcgtct
     1021 cgtagaagac gtgcgtgacc ttgaggcgcc gcgcggtcct cacgatggcg gccaggtcgg
     1081 cggggctcgg ctccgcctcg ggcgcgagtc ccatgaccgg cacctgggtg agccggtagc
     1141 ggcgcgccag gtaggcgtag gcggcgtggg acgtcacgat ctcccggcgc gcgcagtccg
     1201 cgaggccggc cgcgaactcg agatcgagcg cgtcgagccg ggcgatgaag gccttcacgt
     1261 tctccgcgta gaccgcgtgg ccggcggggt cggccagctc gagcccgccc gctatggcca
     1321 gcacctgaac gcgcgcgagc gtcggatcga gccacacgtg gggatcggtg accccgccca
     1381 ccgtgaggag gccgagcccg ccgcgcgcgt tcaccaccac cccgcgccgc ccggtcagat
     1441 cggcgagcag cttctcggcc cagccgtcga ggccggtccc ggtgtggacg aagaccgccg
     1501 cgcgctggat ctggctcacg tcgcgcggcg ccggctccca gtcgtgcggc tcgacgcccg
     1561 ccggcaccag ggagaccact tccgcgcggt cccccgcgac ctggcgcgcg aactcccaca
     1621 gcgggtagat ggtggccacc accagcggct tggcgggcgg cgcggcgggg ccgcagccgg
     1681 ccagcgccag caggaccagc gcgagcgaga gcagccggac ggtcatgcca cgcgccgcac
     1741 gcgcaagacg aggcccaggg ccagcaggct cagaccgaag gcgcagacca cggtcgcgcc
     1801 ggtcgggagg tcgagcacgg ccgaagcgag catggcgacg acgctcacgc ccgcgccgaa
     1861 ggcccagccc accgcgagcc gcgcgccgat gcgctgggcc agcaggatgc ccgcgagcgc
     1921 ggggacgatc aggtacgaga agacgagcag gacccccgcg atgcgcaccg acgaggtcac
     1981 gaccagaccg aacgaggcgt agaagaggaa gtcccacgcg cggacgcgcc agccatcgcg
     2041 ataggcgcgg tccggatcgg tggagatgag gaagaacggc cggcggcaga gccagtgaaa
     2101 gacgccgatc agcgcgtaga gcgcggcgac cttgagcacc tccgcgccgc gcacgctgag
     2161 caggctgccc accagcatcc cgcgcaggtg ctcggcgccg tgcggcgcgc ggtcggccag
     2221 cagcaccgcg ccggccgcgg agaccgcgta gaccacgccg atcaccgcct cgtgggagac
     2281 caggccccgg cggctccggg tgagcgccag caccagcgcg cccaggatcg tgaagccgag
     2341 ccccgccgcg taggactgcc acgtcgcgag ctcgtagccg agcaggaacg ccgcggtggc
     2401 cccgagtgcc gcgatctggg ccagcgcgat gtccacgaac acgacctccc gcgcgatgac
     2461 gtggaagccc aggtacgcgt ggattccggt gagcaccagg cacatcagga acggcatcca
     2521 catcagcgcg agaaggtcgg cggtcatggc ggcctatctt agcccttcgg ccagggtgcg
     2581 cacgttgtag tccacggtct cgagatagct cctggcctcc ttcaccgcgc ccaccgaggg
     2641 cgcgagcacg cgcaccttgg cgcccgcctc ctgggccacg cgctcggcca gcttcacgtc
     2701 gttccagggc tccacgacca cgagcttgat ccgctcctgc ttcatgtcct ggatgaggcg
     2761 ggccaggtgg ccgggagtgg cggggatccc gggccgatcc tcgatggcgc cgcccttgac
     2821 gaggccgaag cgcgtgagga ggtacacgaa gagcgggtgg tactggacga ccttcgcgcc
     2881 cttgaagggt gcgagcgcgg cggtccaccg gcccatcgcc tggtcgagac tcgcgaggaa
     2941 cgcggcgcga ttctgctcga aggccggacg cgactggggt tgcaggcgcg ccagcccgtt
     3001 gaggacgttg gcggtgatct ggggtgccat tccggggtcc atcgtgtagt gcgggttgcc
     3061 gaccgggtgc acgtcaccca gcgagcggtc cacccgggtg gtcggcacct cgagcagcgg
     3121 tagaccttcc gacgcgtcca cgcgaccggg cgtgccgggc agcaccttcg ggttgttggc
     3181 gccctgcacg acgacctcgg cccactggtc gagctcgagg ccgttgatca ccagcaggtc
     3241 ggcgcgccgt accttgacca tcaggctggg acgcacctcg agatcgtgcg cgttctggtt
     3301 gccgcgggcg agcgcctcca cctcgacgag atcgccgccg accgcctcgg tgagcgcctt
     3361 gagatcggtg gtcgtcgtga cgacgcggag cttgtcggcc gccgcggcgt cccccgacac
     3421 gaggccgggg gcgccgaggg cgccaaggga aagagtcagg atcgcggccg cggcggcgcg
     3481 gatcgggatt cgcatggtct ctggcctcct cagaacggat gcgccgggtg ggcgcccagg
     3541 atgaacgtgg cctggaagag gatctcgtcc accttccggg cgctcgactc gttgtcggtg
     3601 aagccgtcgc gggtggcccg ctcggtgtgc ttgtagccca ggcggaagcg caggaactcc
     3661 gacggcgaga atgagaggaa cgggcccacc gcccactccc gcccgcgcat gaccgggtac
     3721 tgggtccagt cgtaacggaa gccggcgccc acgcccttca gccagccggt acggaacggc
     3781 cgcacctccc cgcccgcgta ccagcccaga cgcgtgaacc ggcgcgtctc gtcgggcgtg
     3841 tcctgaaccc cgtcgccgtc ggtatcctcg ccgagcacgt tgatgcggcg ctcctggtag
     3901 atcagctcgc cgagcagcgt gagcagcggc tgctgccagc cctccggcgt gtacttgtac
     3961 ttggcatcga agccgagcag cgtgttgcgg cgccgatctg gtgtctcgcc gttggccacg
     4021 gagacgccga gctggatggc gctccagtcg cccagctcga ggaaggtgcg gagccggccg
     4081 gtgagaagcg gctcgttcag cttcccgcgc ccgaaggcgt cctcgttgtc gccgttgaag
     4141 acgccgacca ggccctccag gaagaagggt aggggcgcga cccacgtcag ctccgcgccc
     4201 cgctcgacga gaccctcggc gccgaggaac cagcggagcg cgttgggggt gtcgatgaaa
     4261 ggccggtcgt gctcgtggat cgggttgagc aggccgaagc ggttgcgcat ctggcccatc
     4321 ttgagctgcg tgttgaacgg cagcgcgagg agggtcaggt gggcttcggc caggtgcagg
     4381 gcgatctcgc ccgcgtcctc ctcgcccgcc tcgatgcgca cctccgcgcg ggcatacgga
     4441 tcgatctggc cgaagagggc gagctcgacc tcgcgcggga agaagcggtt ctcctgcccg
     4501 atgaacgtgc ccgcgttggc cttgtccacg ttgcgctggg tgaagttgcc gatgaagtca
     4561 cccgcgatgc ccatgtcgaa caggagctgg ccgggcccgc ggttgccgta gagcccgaag
     4621 ggctggcggg gccgcgccag gtcgagcggg gtcaccgcga tcgcgggtgc cgccggcgcg
     4681 ggggcctggg ccacactcgg cgcgggcgcg ggggccggcg cggccaccgc cggctgcggc
     4741 cgggtctcga gctgctggat ccgctcgccg aggcggtcga tcgccttctc gtatccctcc
     4801 ttcatcgtct cgaactgctt gcgcatctgc tgcagctcct cgcggagcgc ctcgacatcc
     4861 tgcgcggccg cgggggcggt catgagcacg cttgcggaga gcaggccgat tcccaccagt
     4921 gagcgcatac acgcctccgt ctcgaggaag atggatggcc cgggcgggac agaccgagcc
     4981 gtccgggcga gcgctaccga gcgagcgaga cggagctagg cgggaggagc gcgggagccg
     5041 gcgaggcgga gcgcgggagc gccggggcgg ctgcggggag gaagcgcgca cgccgcggtg
     5101 atgagcgcga gcgagacggc cgcgggcaga tcgggcaggg gcccgtcgcc gcccaggaac
     5161 tcgaggctcg ccaggacgtg atcctcgttg tagacgccgg cggtcgaggc cgcgtggacg
     5221 tggcgcggat agctcgcctt gagcggcagg gtcaccgcca cgagcgcgag cagcagcgcg
     5281 gcggcggcga gacggagcga tccgagcggg cggaggttcc tcactggccc tgtgagtata
     5341 ccgaacggcg aggccgcgcg aaccctctag tcgggactcc gagtcaaggg gtaccgacgc
     5401 ttgagccagc cgtcgtaccc ctcctggagg acgacgtggt tcgtgtagcc cttcgtctcg
     5461 aggaagctgt aggcggtggc cgcctcctcg agcgggcagc ggcagtagag caccaccatg
     5521 ggctgggtcg ggatctccgc gaaacgccgg tcgagctcgg cgagggggac cgagacggcg
     5581 cccggcaggt ggccgcggcg gaagtcgccc gggctgcgca ggtccaccac gaccaccggc
     5641 tggcgggagt cgagcagcca cttgaggtag tcgggctcgg tgagggccac cgtcggcacg
     5701 ccgccgtgac cggcgagcgc ggctcgcgtc agcgcgagcg tgatcgccca gaccacggcc
     5761 cggaccagcg cggaccgcct cgtcacggct gcttcgcgat ggcggtgatc tccacgttgg
     5821 gcggcgcccc ggtgagcgtg aagatgaccg tgtcgccgat gtcgagcccc tcgtagagct
     5881 tgggatcgtg cacgcggaag cccatggtca tcgagcccat gtagccggcc aggtcctcgt
     5941 gcgtgagcac caccacgttg agctcgggca gcaccgcgcg caccacgccg cgcgcctgcc
     6001 aggcgcgccg gctgcccgcg gtggcctgct gcaggcgcgt ccgctccagc tcctggccga
     6061 gccgggccgc ctctcggccc cagcccaggt atccgcagag caggcccacg cccagcgcga
     6121 gattgaccag caggacgacc cgccagagtc gcatggcgcc ctagtgtacg tgctcgtcgg
     6181 gaggaggacc cgcgggggtt tccttgtcga gcaggaggat ctgatccacc agggtctgga
     6241 gagtctccca gcccgggccg ggagcccccg gcagccagcg cgcgcgcacg tagccctggc
     6301 ggtcgagcag gaactccacg tggagcagcg gggccgcgcg aggggagagt tcccgcgggc
     6361 ccaggccccg gccgagcagc ccataggtcg ccgcgatctc ggcggcgccg tcggtgatga
     6421 ccggaaagag cacgggcggc cggccgccga gccggcggat gatgtgcgcc ccgtcgtcca
     6481 gcgggatcgc gatgacctcg gtgccggtgg cctgcagcgt gttgtaggac tcggcgagct
     6541 ggacgagccg ggcccgcgag tccggcaggg tgaagaacac gaggtgcacc atccaacgct
     6601 cgcgcagctc cttgagcccg cgcgggggag ccggtcccac cgcgatggtg aaatcgggcg
     6661 cgacgagcca cgggcgattg ggctggacca ccgccgtcat ctgccgcgcc cgctcccccg
     6721 cggccagggc gcggatgaag ttgatgaggt cccagcgctc ctcctcgccg agcgccgcgg
     6781 cgaatggcgg catgcctccg gcgggaatgc cgtggctgag ccaccagaac atgtcgcccg
     6841 cggtatggtc ggcggcgtgc gccgcggtca ggtcggcggg cagcctcggc aggcctgcgc
     6901 cgccggggcc gtcgcc
//
LOCUS       DASUFA010000385         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_527413, whole genome shotgun sequence.
ACCESSION   DASUFA010000385 DASUFA010000000
VERSION     DASUFA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527413"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VFX87_14080"
     CDS             complement(<1..1292)
                     /locus_tag="VFX87_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4996107.1"
                     /translation="MKCPRCQSQNREGVRFCEECGARLVTSCASCGAELLPDKRFCGS
                     CGSPTTPEPVDRQPSPQGYTPKHLAEKILTSRHALEGERKQVTVLFADLKGSMELLAD
                     RDPEEARRLLDPVLERMMEAVHRYEGTVNQVMGDGIMALFGAPLAHEDHAVRACHAAL
                     RMQQTVGWYAEELRRGRGVDVQIRIGLNSGEVVVRAIDSDLHMDYSAIGQTTHLAARM
                     EQLARPGSTLISRETLRLAEGYVEVKALGPVPVKGMPEPVEVFELVRSEQVHSRLEAS
                     AARGLTPFVGREAEVDALYQAMARAAAGHGQVVAVSGEPGVGKSRLFWEFTHSHRAHG
                     WLMMEAQATSYGKNTSYLTVKELLQNYFHLEERDDVRRVREKVTGKLLTLDESLRPTL
                     PAFLQLLDVPVDDAEWQTPDLPQRRQRMLDAVKRLILR"
     gene            1465..>2723
                     /locus_tag="VFX87_14085"
     CDS             1465..>2723
                     /locus_tag="VFX87_14085"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX4996108.1"
                     /translation="MNATALLAEFVTKSRYEDCPKPALDAARRAILDCLGVMLAGSVE
                     PAARILQQVARTEGGAPLATVVGTGRRTGAVWAALCNGTAAHALDFDDTNFALMGHPS
                     APVLAAALATGELALADGRAVLHAFLLGFEVETTLAEVINPAHYAHGWHATCTLGTLG
                     AAAAAARLLGLDGTQTRHALAVAASQSSGLKENFGTMTKPFHAGHAARSGVLSALMAR
                     EGWTASEHALEGPQGFFSVLGAGKRDLEPLGTLAAPWKILTTGVAVKPYPSCACTHSI
                     IDSALELRRVEGIQADEVEEVTVGVAAGVPRILIHSRPRTGLEAKFSAEFSAAAALID
                     GRVGMATFQDDRAQDPGLRRLMERIRVVVDPEIPTDLERHMWSRMTVRLKGGRSASIS
                     PRPVPGHPANPLTLEALREKFRECAALV"
BASE COUNT          379 a          982 c          966 g          396 t
ORIGIN      
        1 tcccggagaa tgagccgctt gaccgcgtcg agcatgcgct ggcggcgctg cggcaggtcc
       61 ggggtctgcc actccgcgtc gtccaccggc acgtcgagga gctggaggaa cgcgggcagg
      121 gtcggccgca ggctctcgtc gagcgtcagg agcttgccgg tgactttttc gcggacgcgc
      181 cggacgtcgt cgcgctcctc caggtggaag tagttctgca gcagctcctt cacggtgagg
      241 taggaggtgt tcttaccgta ggaggtggcc tgcgcctcca tcatgagcca accgtgcgcg
      301 cgatgggagt gagtgaactc ccagaacagg cgcgacttgc ccaccccagg ctcgcccgac
      361 accgccacca cctggccgtg gccggccgcc gcccgcgcca tcgcctggta gagcgcgtcc
      421 acctcggcct cgcggcccac gaacggcgtg aggccgcgcg cggcgctggc ctcgaggcgc
      481 gagtgcacct gctcggagcg aaccagctcg aagacctcca ccggctccgg catgcccttc
      541 accggcaccg gcccgagggc cttgacctcc acgtagccct cggccagccg cagggtctcg
      601 cgcgagatca gcgtcgagcc ggggcgggcg agctgctcca tacgggcggc caggtgcgtg
      661 gtctggccga tggccgagta gtccatgtgc aggtcgctgt cgatcgcacg caccacaacc
      721 tcgcctgagt tgaggccgat gcggatctgc acgtccaccc cgcggccgcg ccggagctcc
      781 tccgcgtacc agccgacggt ctgctgcatg cggagcgcgg cgtgacaggc gcgtaccgcg
      841 tggtcctcgt gggcgagcgg cgcgccgaag agcgccatga tcccgtcgcc catgacctgg
      901 ttcaccgtgc cctcgtagcg gtgcaccgct tccatcatcc gctcgagcac cgggtccagc
      961 aggcgccggg cctcctccgg atcccggtcg gccaggagct ccatcgagcc cttgaggtcg
     1021 gcgaagagca ccgtgacctg cttgcgctcg ccctcgagcg cgtgccgcga ggtcaggatc
     1081 ttctcggcga ggtgcttcgg ggtatagccc tggggggagg gctggcgatc caccggctcc
     1141 ggggtggtcg gcgagccaca ggagccgcag aatcgcttgt ccggcagcag ctccgcaccg
     1201 cagctcgcac agctcgtcac caggcgcgcg ccacactcct cacagaaccg gacgccctca
     1261 cggttctggg actggcagcg gggacacttc acctagccct cctcgggcgg cgacgccgga
     1321 tggtctcgca agagtagccc ctcccccaat gggggtcaag ggcggggccc ccgacgccat
     1381 gggcgtactc ggcgagggag atcgagacgc tcgacgggct gcccccgggt gtggtgggcc
     1441 gggggctgtg ataggctccc gctcatgaat gccaccgcgc tgctcgcgga gttcgtcacc
     1501 aagagccgct acgaggactg ccccaagccg gccctcgacg ccgcccggcg cgccatcctc
     1561 gactgcctgg gcgtgatgct ggccggctcc gtcgagccgg cggcgcggat cctccagcag
     1621 gtggcccgga cagagggcgg cgccccgctg gccacggtgg tgggcacggg ccgccgcacc
     1681 ggcgcggtgt gggccgcgct ctgcaacggc accgcggctc atgcgctcga cttcgacgac
     1741 accaacttcg ccctgatggg ccatccttcg gcgccggtgc tggcggcggc gctggccacg
     1801 ggcgagctgg cgctggccga cgggcgcgcg gtgctgcatg ccttcctgct gggcttcgag
     1861 gtcgagacca cgctggccga ggtcatcaac ccggcccact acgcccacgg ctggcacgct
     1921 acctgtacgc tcggcacgct cggcgcggcg gcggcggcgg cgcggctgct cgggctcgac
     1981 ggcacgcaga cccgtcacgc cctcgcggtg gccgcctcgc aatcctcggg gctcaaggaa
     2041 aacttcggca ccatgaccaa gcccttccac gccggccacg ccgcgcggag cggtgtgctg
     2101 tcggcactga tggcgcgcga gggctggacc gcctccgagc acgcgctgga aggaccgcag
     2161 ggcttcttca gcgtgctggg cgcgggcaag cgcgatctcg agccactcgg gacgctcgcc
     2221 gcgccgtgga agatcttgac caccggagtc gcggtgaagc cctacccctc ctgcgcgtgc
     2281 acgcattcga tcatcgacag cgcgctcgag ctccggcggg tcgagggcat ccaggccgac
     2341 gaggtcgagg aggtgacggt cggcgtggcc gcgggtgtgc cgcgcatcct gatccactcg
     2401 cgcccgcgta ccggtctcga ggcgaagttc tccgccgagt tctcggccgc ggccgcgctg
     2461 atcgacggac gcgtcggcat ggccaccttc caggacgacc gcgcgcagga tccgggcctc
     2521 cggcgtctga tggagcggat ccgggtggtg gtggatccgg agattcccac cgatctcgag
     2581 cgccacatgt ggagccgcat gaccgttcga ttgaagggcg gccgatcggc cagcatcagc
     2641 ccgcggccgg ttccgggcca tcccgccaat ccgctgacac tcgaagcgct gcgggagaag
     2701 ttccgggagt gcgccgccct ggt
//
LOCUS       DASUFA010000386         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_777365, whole genome shotgun sequence.
ACCESSION   DASUFA010000386 DASUFA010000000
VERSION     DASUFA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777365"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFX87_14090"
     CDS             complement(<1..627)
                     /locus_tag="VFX87_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996109.1"
                     /translation="MREPASIRILVWMCVLIAVNQLGFGSIIPALALYARSFDVTQSA
                     IGLAIAVYGLARFLIAVPAGQLADRLGRRNTLAMGGLLSAVGNVLCALAPNYPAFVGA
                     RFVAGAGAALVLTTGLIVIADISTPANRGRMMSAYQGAFIFAVGIGPLPGGLLATHWG
                     LAAPFVAYAATSLVASAVAWLAIPETRAVRRADEPEPAHLPYRDQLRLL"
     gene            714..1496
                     /locus_tag="VFX87_14095"
     CDS             714..1496
                     /locus_tag="VFX87_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996110.1"
                     /translation="MATGTAKGFAVFAGVTPEIIRASAREAEALGYRSFWVNHPGPVD
                     GLGSLAHAAAETRRVDLGVGVIPLHTRGPESIADGVRGHGLAPERLLLGVGSPNPEAL
                     KRVRDGVAALRAKLSTRIVVAALGPRMCQLAGEVADGVLFNWLTPEYARRSAELVRAG
                     ADAAKRPMPRLYAYVRVALGPAAAERLADEGGRYDSIPAYHAHFVRMGVKPVDTAIAA
                     ASAEGIKRALAAWDGAVDEVVLRAITAKDTVEDHLTLVRAAG"
     gene            complement(1576..2598)
                     /locus_tag="VFX87_14100"
     CDS             complement(1576..2598)
                     /locus_tag="VFX87_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX4996111.1"
                     /translation="MTLPSTLLPTSVIGSHGLPGWVWLAREALQAGRLGATDVRELME
                     DATQVALLDQERAGIDVLTTGEMGRVRFIIGFYDHLRGIRTMVEPRKLGQPHWDTNTP
                     FEVVERIAAPAGLGIVDEFKLARSLTARPLKATVPGPYTLMFPLRLGGGYRDRDTLLA
                     DLVGIVNAECRALVAEGADFIQIDEPHSGMYAGSAPNFAQGVNRAVEGVRAKIAVHVC
                     FGNLYGRPFQPVRSYRNVYPAIHELTASQVVLEYANRGMDDLALWKEFPTDKELGAGV
                     IDVKAFKAETAEEVAERIRALLRYVAPDKLWLNPDCGFWETPRWATRLKMTALVEGAR
                     LVRREL"
     gene            complement(2595..3353)
                     /locus_tag="VFX87_14105"
     CDS             complement(2595..3353)
                     /locus_tag="VFX87_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4996112.1"
                     /translation="MDAVRLELDGPVARITLNRPEVLNAGDPGWVAELTDVVARVGSL
                     RELRVAVITGAGRAFSTGVDLRSLAAGEMTLPHFVAWEDAMTAIERMRIPFIAAINGH
                     CLGGGLQLALVCDYRLAAADAMIGLPAVKECLIPSMALYRLPRLIGAARAREMILTGE
                     PISAERAADYGLVNQIVPASEFPRALDACIGRFLALPATAVAVSKSLLARTFDLPFDA
                     FRREMEAGFGLCLDSTEHRAAMDEILRRRKRPAR"
     gene            complement(3353..>4387)
                     /locus_tag="VFX87_14110"
     CDS             complement(3353..>4387)
                     /locus_tag="VFX87_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX4996113.1"
                     /translation="PGRLAVVTIVAGTVFSAISGSTIATTALLGSLMLPEMLRRGYHP
                     RMAMGPIMAIGGVDMLIPPSALTVLLGSLAGISISQLLVAGIVPGVLLSVLFVGYIVT
                     RTILDPALAPPFEGERVTGWARWWPLVVYVLPLLLIFGVVVLAMSAGWATPTESAAVG
                     AVATALIAALYRSLSWPSLLRSLLGTAGISGVILLIIVGATTFSQILAFSGATNGLVT
                     VIREAQLTPVVVLWAMMLVLLVLGCFIDQVSMMLITLPFFMPLVELYRFDPVWFGVMF
                     LICLQLGLLTPPFGMLLFTMKSVAPREISMGQVYRAVTPYVLFGLVMLAVVWLVPPVA
                     TWLPRLLFGK"
BASE COUNT          720 a         1459 c         1661 g          547 t
ORIGIN      
        1 gaggaggcgg agctggtcgc ggtagggcag gtgggcgggc tcgggctcgt cggcgcgccg
       61 gacggcgcgc gtctcgggaa tcgcgagcca ggccaccgcg gaggccacca gcgacgtcgc
      121 cgcgtaggcg acgaaggggg cggcgaggcc ccagtgggtg gcgagcaggc cgcccggcag
      181 aggcccgatg ccgacggcga agatgaacgc gccctggtag gccgacatca tgcgcccgcg
      241 attggcgggc gtggagatgt ccgcgatcac gatgaggccg gtggtgagga cgagcgccgc
      301 gcccgcgccc gccacgaagc gggcgccgac gaaagcggga tagttcggcg cgagcgcgca
      361 gagcacgttg ccgaccgcgg agaggagccc gcccatcgcc agcgtgttgc ggcgcccgag
      421 ccgatccgcg agctggcccg ccggcaccgc gatcaggaag cgggcgagcc cgtagaccgc
      481 gatggcgagg ccgatggcgg actgcgtgac gtcgaaggag cgtgcgtaga gggccagcgc
      541 ggggatgatg ctgccgaagc cgagctggtt gacggcgatg agcacgcaca tccagacgag
      601 gatgcggatg ctcgcgggct ctcgcatcag agcttgggtg tatcccgtaa tgccgtggag
      661 cgcaagcccg ccgaggcgta tgatcgggcg caccacacga agaggaggcc gtcatggcga
      721 ccggcacggc aaagggattc gcggtattcg caggcgtgac accggagatc atccgcgcct
      781 ccgcccggga ggccgaagcg ctcggctacc gctcgttctg ggtgaatcac ccggggccgg
      841 tggacggcct gggatcgctc gcgcacgcgg cggcggagac gcggcgcgtg gacctgggcg
      901 tgggcgtcat cccgctgcac acgcgcgggc cggagagtat cgcggacggc gtgcgcgggc
      961 acgggctcgc gccggagcgc ctgctgctgg gcgtgggcag cccgaatccg gaagcgctca
     1021 agcgcgtgcg tgacggcgtg gccgcgctgc gggccaagct gtccacgcgg atcgtcgtgg
     1081 ccgcgctcgg gccccggatg tgccagctcg cgggcgaggt cgccgacggg gtgctgttca
     1141 actggctcac gccggagtac gcccggcgct cggcggagct cgtgcgcgcc ggcgcggatg
     1201 cggcgaagcg gccgatgccg cggctctacg cctacgtgcg ggtggcgctg ggcccggcgg
     1261 ccgccgagcg cctggccgac gagggcgggc gctacgactc cattcccgcc taccacgcgc
     1321 atttcgtccg gatgggcgtc aagccggtgg acacggccat cgcggccgcc tccgccgagg
     1381 ggatcaagcg cgcgctcgcg gcctgggacg gcgcggtgga cgaagtggtc cttcgcgcga
     1441 tcaccgccaa ggacacagtg gaagatcacc tcaccctggt ccgcgcggcc ggctagaccg
     1501 gggtcaggtc ttacattcct acatttcgtc tgacgcgacg gtgcgcagat gtcggattgt
     1561 aagacctgac cccggctaga gctcgcggcg gacgaggcgg gcgccttcga cgagggcggt
     1621 catcttgaga cgggtggccc agcggggggt ctcccagaag ccgcagtcgg gattgagcca
     1681 gagcttgtcg ggagcgacgt agcggaggag tgcacggatg cgctccgcga cctcctcggc
     1741 ggtctccgcc ttgaaggcct tcacgtcgat cacgccggcg cccagctcct tgtcggtcgg
     1801 gaactccttc cagagcgcga ggtcgtccat gccgcggttg gcgtattcga gcaccacctg
     1861 cgacgcggtc agctcgtgga tggccgggta cacgttgcgg tagctgcgca ccggctggaa
     1921 cgggcggccg tacagattgc cgaagcaaac gtggacggcg atcttggccc gcacaccctc
     1981 gaccgcgcgg ttgacgccct gagcgaagtt gggcgcggag cccgcgtaca tgccgctgtg
     2041 gggctcgtcg atctggatga agtcggcgcc ctccgccacc agcgcgcggc actccgcgtt
     2101 gacgatgccc accaggtcgg ccagcagcgt gtcgcgatcg cggtagccgc cgcccagccg
     2161 tagcgggaac atcagcgtgt aggggcccgg aacggtggcc ttgagcggcc tggcggtcag
     2221 cgaccgcgcc agcttgaact cgtccacgat gccgagcccg gccggggccg cgatgcgctc
     2281 aacgacctcg aacggcgtgt tggtgtccca atggggctgc ccgagcttgc ggggctccac
     2341 catggtgcgg ataccgcgga gatggtcgta gaagccgatg atgaagcgca cccgccccat
     2401 ctcgccggtg gtcagcacgt caatcccggc ccgctcctga tcgagcagcg cgacctgggt
     2461 ggcgtcctcc atcagctcgc ggacgtcggt ggcgcccagc cgcccggcct ggagcgcctc
     2521 gcgggcgagc cagacccagc cgggcagacc gtggctgccg atgaccgagg tggggaggag
     2581 cgtcgagggc agggtcatcg cgcgggtctt tttctccgcc ggaggatctc gtccatggcg
     2641 gcgcggtgct cggtggaatc gaggcagagc ccgaagcccg cctccatctc ccggcgaaag
     2701 gcgtcgaacg ggagatcgaa ggtgcgcgcg aggaggctct tgctcacggc caccgcagtc
     2761 gcggggaggg cgaggaagcg tccgatgcac gcgtcgagcg cccgggggaa ctcggatgcg
     2821 gggactatct gattgacgag cccgtagtct gcggcccgct ccgccgagat cggctcgccg
     2881 gtcaggatca tctcgcgcgc ccgcgcggcg ccgatcagcc gggggagccg gtagagcgcc
     2941 atggacggga tcaggcactc cttcaccgcg ggcaggccga tcatcgcgtc cgccgcggcc
     3001 agccggtagt cgcagaccag cgcgagctgg agccccccgc ccaggcagtg gccgttgatg
     3061 gccgcgatga aggggatccg catgcgctcg atcgcggtca tcgcgtcctc ccaggccacg
     3121 aagtgcggca gcgtcatctc ccccgcggcg agcgaccgga gatccacgcc ggtcgagaag
     3181 gcccgtccgg ctccggtgat caccgcgacc cggagctcgc gaagcgaccc gacgcgcgct
     3241 accacgtcgg tcagctcggc cacccagccc ggatcccccg cgttcagcac ctcgggccgg
     3301 ttcagggtga tgcgggccac gggcccgtcg agctcgaggc ggaccgcgtc catcacttgc
     3361 cgaagaggag gcgcggcagc caggtggcca ccggcggcac gagccagacc accgcgagca
     3421 tcacgaggcc gaagagcacg tagggcgtga ccgcgcggta gacctggccc atggagatct
     3481 cgcggggcgc cacgctcttc atggtgaaga gcagcatccc gaacggtggg gtgaggaggc
     3541 cgagctggag gcagatgagg aacatgacgc cgaaccacac gggatcgaag cggtagagct
     3601 cgacgagcgg catgaagaag ggcagggtga tcagcatcat gctcacctgg tcgatgaagc
     3661 agcccagcac gagcagcacc agcatcatcg cccagagcac caccaccggg gtgagctgcg
     3721 cctcgcggat cacggtcacg agcccgttgg tggcgccgga gaacgcgagg atctgcgaga
     3781 aggtggtggc gcccacgatg atgagcagga tgaccccgga gatgcccgcg gtgccgagga
     3841 gcgacctgag gaggctcggc cacgagagcg accggtagag cgccgcgatc agcgcggtag
     3901 cgaccgcgcc gaccgccgcc gattcggtgg gcgtggccca gcccgcggac atggcgagga
     3961 cgacgacccc gaagatgagc agcagcggca gcacgtagac cacgagcggc caccagcgcg
     4021 cccagcccgt cacccgctcg ccctcgaagg gcggggccag cgccgggtcg aggatggtcc
     4081 gcgtgacgat gtagcccacg aagagcaccg acaggaggac gccggggacg atcccagcca
     4141 ccaggagctg ggagatcgag atgccggcca ggctccccag cagcacggtg agggccgagg
     4201 gcgggatcag catgtccacg cccccgatgg ccatgatcgg gcccatcgcc atgcgcgggt
     4261 gatagccccg gcgcagcatc tcgggcaaca tcaggctgcc cagcagcgcg gtggtcgcga
     4321 tggtcgagcc cgagatggcg gagaacacgg tgccggcgac gatggtcacc accgccaggc
     4381 gcccggg
//
LOCUS       DASUFA010000387         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1348191, whole genome shotgun sequence.
ACCESSION   DASUFA010000387 DASUFA010000000
VERSION     DASUFA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348191"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="VFX87_14115"
     CDS             <1..1923
                     /locus_tag="VFX87_14115"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4996114.1"
                     /translation="FVQGDPAAILIVEYAGETEQEVKAKVADLEARRTRGRFGYASHI
                     ATDSAEQQSIWKLRKAGLGLLLGTRGESKPIAFIEDTAVDPKHLPEFVPQFTAIMAKH
                     GADAAYYGHCSVGCLHIRPLINLKTERGLEQVRVMAEEIFDLVLSFGGAISSEHGDGR
                     ARSPFLERVFGPTMFRAFKETKAAFDPRGLMNPGNIVGSPGVTEHLRYGAQYKTWEPK
                     TLLDFSAQGGFAAAVEMCNGVGVCRKKLEGTMCPSYMATRDEEHTTRGRANALRAVLS
                     GQVPATDFAGKRLYEVMDLCLECKGCKAECPSNVDMAKMKYEFLHHYHSVNGQPLRNR
                     LFGHIARLSWLGSRLAPISNWIARSWPNRLLMEWVAGIDRRRPLPGFARETFTDWFDR
                     RRAPGEAPRGDVVLFHDTFITYNAPEIGRAAVELLEAAGYRVQLVDKKCCGRPLISKG
                     MLDEAKVHAEWNVARLLPWVDRGAAIVGLEPSCLLTLRDETVELVRTDEARRVAASAF
                     LLEEFLLRERAHGLTLPFANGHVRKQALLHGHCHQKAMVGTAPTVAALRWAGYEVAEV
                     DSGCCGMAGSFGFEREHYDVSVTLGNRRLAPAVKAATAETEIVAPGVSCRQQIEHLAG
                     RQAKHPAQVLREALRS"
     gene            1958..2743
                     /locus_tag="VFX87_14120"
     CDS             1958..2743
                     /locus_tag="VFX87_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996115.1"
                     /translation="MDNLTLKLVVTPALIGAATLAGRRWGQSIGGWLVGLPLTTGPVA
                     FFIALDHGESFAAAAVVGSLAGAVAEVAFSLAYGWSALRYSWPPALLAGTVAYAAVAT
                     LVQGLALGAVALFALVIVALVLSLRLMPRGAPGVGPMMAPRWDLPARMVLATTVVLVL
                     TGLAPRLGARWSGLLATYPLFAAILTAFGHRLQGAGAAIGVLRGLLFGLFSFAGFCLV
                     LALGLVPLGIAGAFAAAITVALLAQGISLWRLRTPLTLPRRHL"
     gene            2842..>3101
                     /locus_tag="VFX87_14125"
     CDS             2842..>3101
                     /locus_tag="VFX87_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4996116.1"
                     /translation="MRPARAQDQALLRVLRAGICGTDLHIFQGHLDHRIPKGGIIGHE
                     TFAEVVEAPAGSGFGPGDRVVVEPIHACGECRACRMGGYYLC"
BASE COUNT          396 a         1116 c         1118 g          471 t
ORIGIN      
        1 ttcgtgcagg gcgaccccgc cgccatcctg atcgtggagt acgcggggga gaccgagcag
       61 gaggtcaagg ccaaggtcgc ggacctcgag gcgcggcgga cgcggggtcg cttcggctac
      121 gcctcgcaca tcgccaccga ctcggccgag cagcagtcca tctggaagct gcgcaaggcc
      181 ggcctcggcc tgctcctggg cacgcgcggc gagtccaagc ccatcgcgtt catcgaggac
      241 accgcggtgg acccgaagca cctgcccgag ttcgtgccgc agttcaccgc gatcatggcg
      301 aagcacgggg cggacgccgc ctactacggc cactgctcgg tggggtgcct gcacatccgc
      361 ccgctgatca atctcaagac cgagcgcggc ctcgagcagg tgcgggtcat ggccgaggag
      421 atcttcgacc tcgtgctctc cttcggcggg gccatctcga gcgagcacgg cgacggccga
      481 gcccgcagcc cgttcctgga gcgggtgttc gggcccacca tgttccgggc cttcaaggag
      541 acgaaggcgg ccttcgatcc gcgcgggctg atgaacccgg gcaacatcgt gggcagcccc
      601 ggcgtcaccg agcatctccg ctacggcgcc caatacaaga cgtgggagcc caagaccctg
      661 ctcgacttct cggcgcaggg cggcttcgcg gccgcggtgg agatgtgcaa cggggtcggg
      721 gtctgccgca agaagctcga gggcaccatg tgcccgtcct acatggccac ccgggacgag
      781 gagcacacca cccgcgggcg cgccaacgcg ctgcgggcgg tgctgtcggg ccaggtgccc
      841 gcgacggact tcgcggggaa gcggctctac gaggtgatgg atctctgtct ggagtgcaag
      901 ggctgcaagg cggaatgccc gtccaacgtg gacatggcca agatgaagta cgagttcctg
      961 caccactacc attccgtcaa cgggcagccg ctgcgcaacc ggctgttcgg gcacatcgcg
     1021 cggctctcct ggctcggctc gcggctggcc ccgatctcca actggatcgc gcgctcctgg
     1081 ccgaaccgcc tgttgatgga gtgggtcgcc ggcatcgacc ggcggcggcc gctgcccggc
     1141 ttcgcgcggg agaccttcac cgactggttc gatcggcggc gcgcgcccgg tgaggccccg
     1201 cgcggcgacg tggtgctctt ccacgacacc ttcatcacct acaacgcgcc ggagatcggg
     1261 cgggccgcgg tggagctgct ggaggcggcg ggctaccgcg tccagctcgt ggacaagaag
     1321 tgctgcggcc ggccgctcat ctccaagggc atgctcgacg aggccaaggt ccacgccgag
     1381 tggaacgtgg cgcgcctgct gccctgggtg gaccggggcg cggccatcgt cggcctcgag
     1441 ccctcctgcc tcctcacgct gcgggacgag accgtcgaac tcgtgcgcac cgacgaggcg
     1501 cgccgggtgg cggcctccgc ctttctcctg gaggaattcc tgctgcgcga gcgcgcgcac
     1561 ggcctcacgc tgcccttcgc gaacggccac gtgcgcaagc aggcgctcct ccacggccac
     1621 tgccatcaga aggccatggt cggcaccgcg ccgacggtcg ccgcgctgcg atgggccggc
     1681 tacgaggtgg cggaggtgga ctcgggctgc tgcggcatgg cgggctcctt cggcttcgag
     1741 cgcgagcact acgacgtctc ggtgacgctg ggcaaccggc ggctcgcgcc cgcggtgaag
     1801 gcggcgaccg ccgagaccga gatcgtggcg cccggggtct cctgccgcca gcagatcgag
     1861 cacctggcgg gccgccaggc caagcacccc gcccaggtcc tgcgcgaagc gctccgctcc
     1921 tgagcccggc gtcccgcgga cgccggccgc gcccccgatg gacaacctga cgctcaagct
     1981 cgtggtgacg cccgccctga tcggggcggc cacgctcgcg gggcggcgtt ggggccagtc
     2041 catcggcggc tggctggtgg gcctgccgct cacgaccggt ccggtcgcgt tcttcatcgc
     2101 gctcgatcac ggagagtcct tcgcggcagc cgcggtggtg ggctcgctgg cgggcgccgt
     2161 cgcggaggtc gccttcagcc tggcgtacgg ctggagcgcg ctccggtact cgtggccgcc
     2221 ggccttgctg gccggcaccg tggcctacgc ggccgtcgcg acgctggtgc agggcctggc
     2281 gctcggagcc gtcgccctgt tcgcactggt gatcgtcgcg ctggtgctct cgctccggct
     2341 catgccgcgg ggcgcccccg gggtcgggcc gatgatggct ccccgctggg acctgccggc
     2401 gcgcatggtg ctcgccacca cggtggtgct cgtcctcacc gggctcgcgc cgcggctcgg
     2461 cgcccggtgg agcgggctgc tcgcgaccta tccgctcttc gcggccatcc tgaccgcgtt
     2521 cggccaccgg ctccagggcg cgggcgcggc gatcggcgtg ctgcgcggcc tgctcttcgg
     2581 cctgttctcg ttcgcgggct tctgcctggt gctggcgctg ggcctggtcc cgctcgggat
     2641 cgcgggcgcg ttcgccgccg cgatcacggt tgccctgctg gcgcagggga tctcgctgtg
     2701 gcggctgcgg acccccttga ctctgccccg tcgccacctc tagatagtcc cggcgtcact
     2761 cgcccggcac ccgaccaagg agactcgcgc atgagagcgt ccctgtacga aggctcccgg
     2821 atttttcgca ctgtgcccgc gatgcgcccg gcgcgtgccc aggaccaggc gctgctccgc
     2881 gtgctgcgcg cgggcatctg cggcaccgat ctgcacatct tccaggggca cctcgatcac
     2941 cgcatcccga agggcggcat catcgggcac gagaccttcg cggaggtggt ggaggcgccg
     3001 gcaggcagcg gcttcgggcc cggggatcgc gtggtggtgg agccgatcca cgcctgcggc
     3061 gagtgccgcg cctgccggat gggcggctac tacctctgct a
//
LOCUS       DASUFA010000388         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_908244, whole genome shotgun sequence.
ACCESSION   DASUFA010000388 DASUFA010000000
VERSION     DASUFA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908244"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="VFX87_14130"
     CDS             complement(<1..1508)
                     /locus_tag="VFX87_14130"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HEX4996117.1"
                     /translation="MSATPPIVRPPVARKIPRTDVVHGDVRQDDYHWMREKDDPEVIA
                     YLTAENAYTDAVMKPTVPFQESLYSEMLARIKEDDQSVPYRLRGYYYYSRTEKGRQYP
                     IYCRKAGSLEAPEQVMLDLNRLAEGHPFLALGAAAVSDDGRRLAYTTDVTGFREYTLH
                     VKDLETGALLPDRVEKVSAVAWAADNETLFYVTEDEAKRPYRLHRHRLGVAGADLLHE
                     EPDALFRFHVGRSRSLAYLFLISGSFTSTEVRYLAAGDPGGAWRMVLPREPDHEYQVD
                     HGREADGDVLYIRTNGGGRRNFRLVVAPVDDPHPTRWRELIAHRPDVMLEDVDVFARH
                     YVVHEREDGLIRLRVTDARTGAYHHVEFPEPTYDLSSDANAEFDAATYRVRYQSLITP
                     ASVFDYEIAGRRLLLLKQAEVLGGYDPARYVSERIHATAGDGTRVPISLVRRAASPLD
                     GSSPMLLAGYGAYGYPYPAGFSSNRLSLLDRGVSVAIAHIRGGGELGKRWHD"
     gene            1581..2483
                     /locus_tag="VFX87_14135"
     CDS             1581..2483
                     /locus_tag="VFX87_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX4996118.1"
                     /translation="MPELPDVTVYLEALAARIGGARLERLRLANPFVLRSVDPGPGEV
                     AGRAVTGLRRLGKRIVIALEGELFVLIHLMIAGRLHWKEAGAKLPGKIGLAALDFSTG
                     TVTLTEAGSKRRAAIHLIRGEAALAAHDPGGLELGAADAAAFRAAITRESHTLKRTLT
                     DPRILSGIGNAYSDEILHRARLSPVRMTRQLSEEESTRLFEAAKATLADWIERLRRAA
                     GGEFPEGVTAFRPDMAVHGRYGRPCPDCGAPVQRIVHAENETNYGARGQTAGRLLADR
                     ALSRLLHQDWPRTLEEMEERRRAP"
     gene            complement(2499..3017)
                     /locus_tag="VFX87_14140"
     CDS             complement(2499..3017)
                     /locus_tag="VFX87_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996119.1"
                     /translation="MRSTVGTMLLVGLLAGALAACSSSSAGLPYKPAVQPPGATVSAA
                     YTIVSDRLRVEIDGRGRRVERAQIVKADGAVLEAQTIEYTPAASGGGSPVSIGFGIGS
                     FGSVGGGVSTGVGVSTGGVVGGGGSSSEVGSSIASFPLAQAGPSPWRVRVKLQGTDPA
                     DILVGEPGPAPR"
     gene            3048..>3586
                     /locus_tag="VFX87_14145"
     CDS             3048..>3586
                     /locus_tag="VFX87_14145"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin family protein"
                     /protein_id="HEX4996120.1"
                     /translation="MTGDEEERVPPGADLERYVRELAEFVGLDEADTAAIRASAPAIL
                     KHEAALTGALYEHFLRFPATARFFLGPDGAPDHTRIDRRKHSLARWLRETAQAALTHD
                     FAYYLLSVGLAHSHRDHGPGGKIPPQFMVAAMSLTQTALARIFRDELGDPAAALEAAV
                     AWNKLLLLHLNVLLIGYLLP"
BASE COUNT          497 a         1322 c         1271 g          496 t
ORIGIN      
        1 tcgtcgtgcc agcgcttgcc cagctcgccc ccgccccgga tgtgggcgat cgccacgctg
       61 accccgcggt cgagcaggct cagccggttc gacgagaagc cggccgggta gggatagccg
      121 taggccccgt agcccgcgag cagcatcggg ctcgagccgt ccaggggcga ggcggcgcgg
      181 cgcacgagcg agatggggac gcgggtgccg tcgccggccg tcgcgtggat gcgctcggag
      241 acgtaccgcg cgggatcgta gccgcccagc acctccgcct gcttgagcag gagcagacgg
      301 cgccccgcga tctcgtagtc gaacaccgac gcgggcgtga tcagcgactg gtagcgcacc
      361 cggtaggtcg cggcgtcgaa ctccgcgttg gcgtccgagc tcaggtcgta ggtcggttcc
      421 gggaactcca cgtgatggta cgcgccggtg cgcgcgtcgg tgacgcggag ccggatcagc
      481 ccgtcctcgc gctcgtggac gacgtagtgc cgggcgaaca cgtccacgtc ctcgagcatc
      541 acgtccggac gatgcgcgat cagctcgcgc cagcgcgtgg gatgcggatc gtccaccggg
      601 gccaccacca gccggaagtt ccgccggccg ccgccattcg tgcggatgta caagacgtcg
      661 ccgtccgcct cccgcccatg gtcgacctga tattcatggt ccggctcccg cggcagcacc
      721 atgcgccacg ccccgcccgg gtcgccggcg gcgagatagc gcacctcggt gctcgtgaag
      781 ctgcccgaga tcaggaacag gtaggccagg ctgcgcgagc ggcccacgtg gaagcggaag
      841 agcgcgtccg gctcctcgtg cagcaggtcg gcccccgcga cccccaggcg gtgccggtgc
      901 agccggtagg ggcgcttggc ctcgtcctcg gtgacgtaga agagcgtctc gttgtccgcg
      961 gcccaggcca ccgcgctcac cttctccacg cggtcgggca gcagcgcgcc cgtctcgaga
     1021 tccttcacgt ggagcgtgta ctcgcggaac ccggtcacgt cggtggtata ggcgagccgc
     1081 cggccgtcgt cgctcaccgc ggccgcgccc agcgccagga aggggtgccc ctcggccagc
     1141 cggttgaggt cgagcatcac ttgctcgggc gcctcgaggc tgccggcctt tcggcagtag
     1201 atcgggtact gcctcccctt ttcggtgcgc gagtagtagt agtagccgcg gaggcggtag
     1261 ggcaccgact ggtcgtcctc cttgatgcgc gccagcatct cggagtagag cgattcctgg
     1321 aagggcacgg tgggtttcat caccgcgtcg gtatacgcgt tttccgcggt caggtaggcg
     1381 atgacctcgg gatcgtcctt ctcccgcatc cagtggtaat cgtcctggcg cacgtccccg
     1441 tggacgacgt cggtcctggg aattttccgg gcgaccgggg ggcggacgat cggcggggta
     1501 gcggacatgc tccgcaccgt accacacggc ccccgcttgc gcggccgggc gctcggtcca
     1561 tacactggcg cgatccggcc atgcccgagc tgcccgacgt caccgtctac ctcgaagcgc
     1621 tggccgcgcg cattggcggc gcgcggctgg agcgcctccg gctcgccaac cccttcgtgc
     1681 tgcgctcggt ggatccggga ccgggcgagg tggcgggccg tgcggtgacc ggtttgaggc
     1741 gcctgggcaa gcggatcgtg atcgcgctgg agggcgagct cttcgtgctc atccacctga
     1801 tgatcgcggg gcgcctgcac tggaaagaag ccggcgcgaa gctgcccggc aagatcgggc
     1861 tggccgcgct cgacttctcc accggcacgg tcaccctcac cgaagcggga tcgaagcgcc
     1921 gcgccgccat ccacctgatc cgcggcgagg cggcgctcgc cgcccacgac ccgggcgggc
     1981 tcgagctcgg ggccgccgac gcggccgcct tccgggccgc gatcacccgc gagtcccaca
     2041 cgctcaagcg gacgctgacc gacccgcgga ttctgagcgg catcggcaac gcttactccg
     2101 acgagatcct gcaccgagcc cggctctcgc ccgtgcgcat gacgcgccag ctctccgagg
     2161 aggagagcac gcggctcttc gaggcggcaa aggccacgct ggccgactgg atcgagcggc
     2221 tgcggcgtgc cgccggcggc gagttcccgg agggcgtgac cgcgttccgg cccgacatgg
     2281 cggtgcacgg ccgctacggc cggccctgtc ccgactgcgg cgccccggtg cagcgcatcg
     2341 tgcacgcgga aaacgagacc aactacggcg cgcggggcca gaccgccggg cgccttctgg
     2401 ccgatcgcgc gctgtcgcgg ctgctccacc aggactggcc gcgcacgctg gaggagatgg
     2461 aggagcggcg gcgcgcaccg tgagggtgcg ccccggcttc agcgcggagc gggcccgggc
     2521 tcgccgacca ggatgtcggc cggatcggtg ccctggagct tgacgcggac gcgccacggg
     2581 ctcggcccgg cctgggcgag cgggaaggac gcgatgctgc tcccgacctc gctgctgctc
     2641 ccgccgccgc ccaccacccc gcccgtcgag acgccgaccc cggtactcac cccgccgccc
     2701 acgctcccga agctgccgat cccgaagccg atgctgaccg ggctgccccc cccgctcgcg
     2761 gccggggtgt actcgatggt ctgcgcctcg agcacggcgc cgtcggcctt cacgatctgc
     2821 gcccgctcga cccggcgccc gcggccgtcg atctcgacgc ggagccggtc gctcacgatc
     2881 gtgtaggcgg cggacacggt ggcgcccggc ggctgcaccg cgggcttgta cggcaggccg
     2941 gccgaggagg atgagcacgc cgcgagcgcg cccgcgagca acccgaccaa gagcatcgtc
     3001 ccgacggtgc tccgcatgca cggtattatc gcacgcggag ggaccccatg actggcgacg
     3061 aagaagagcg agtacccccg ggagccgatc tcgagcggta cgtccgggag ctggccgagt
     3121 tcgtcggcct cgacgaggcg gacacggccg ccatccgcgc gagcgcgccc gcgatcctga
     3181 agcacgaggc cgcgctcacc ggcgcgctct acgagcactt cctgcgcttt cccgcgaccg
     3241 cccgcttctt cctgggcccg gacggggcgc cggaccacac gcggatcgac cggcgcaagc
     3301 acagcctggc ccgctggctg cgcgagaccg cccaggccgc gctgacccac gacttcgcgt
     3361 actacctgct ctcggtcggc ctcgcccaca gccaccgcga ccacgggccg ggcggcaaga
     3421 tcccgccgca gttcatggtg gcggcgatga gcctcaccca gaccgcgctc gcccgcatct
     3481 ttcgcgacga gctgggcgat ccggcggcgg ccctcgaggc ggcggtggcg tggaacaagc
     3541 tgctgctgct ccacctgaac gtgctgctga tcggctacct gctccc
//
LOCUS       DASUFA010000389         7256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1349953, whole genome shotgun sequence.
ACCESSION   DASUFA010000389 DASUFA010000000
VERSION     DASUFA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349953"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFX87_14150"
     CDS             <1..286
                     /locus_tag="VFX87_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX4996121.1"
                     /translation="MTRAGLSPSVAGGFEWSWPDTPPGLDRVAWWVARSAAELLTSPD
                     LAAVRECAGYDCGRLFMDGTKNRSRRWCDMASCGNRAKGRRHYERLKSTR"
     gene            complement(347..1375)
                     /locus_tag="VFX87_14155"
     CDS             complement(347..1375)
                     /locus_tag="VFX87_14155"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX4996122.1"
                     /translation="MAALPLIPSTVVGSHGKAGWWYTAVKAYEAGAMGPGDLEEMFDD
                     AADTAIRDMERAGLDIITDGEVRRLDGYVDSYYAVIKGIEPLPVRRKAGPWGYDQQTR
                     YEATGRIEAPAGGLGIVKEFEYLKAHTTRGVKATCAGPLTFGSRIHPGKMYKGVVDVA
                     ERFAEVINAELRGLVEAGAEFIQLDEPARGNVSGEEMARLFNLATEGVKAKLAFHICF
                     GNRFGRSRFDRSYRPYFPGVLKARAEQLVLEFAGRELSELDLWKEYGQDRELGAGVID
                     VKGFNPESPEDVAARIRRVLAVCRPEKLYVNPDCGFGWSPRYMCNQKVRALAAGAVLA
                     RQQLTGSR"
     gene            1423..1812
                     /locus_tag="VFX87_14160"
     CDS             1423..1812
                     /locus_tag="VFX87_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996123.1"
                     /translation="MSVSAAETHASILSLPQLVNNDAGLVRRGRYLTATFLVEAGDAE
                     FLVRVVEGRLAAVERGPFLMRSWTFALRASAETWQRFWEPTPAPGYHDLFAMKKLGVA
                     RIEGDLTPLMAHLRYVKDVVAAPRRRP"
     gene            1813..2673
                     /locus_tag="VFX87_14165"
     CDS             1813..2673
                     /locus_tag="VFX87_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4996124.1"
                     /translation="MTEPIFEPIKGTYMRLSLGGRPHRLYFEEAGQGIPLVCLHTAGA
                     DGRQFRHLLNDEAITRHFRVLAFDMPWHGKSLPPAGWQDEEYRLTTRGYVDLILAFCA
                     ALGLERPVVLGCSIGGKIVLELARTHPECFRALIGLESAAYQPPWYDDTGWLHRPDVH
                     GGELCGALMSGLIAPQSPDETRWETLWGYMQGGPGVFKGDLHFYRVDGDLRDRVGQID
                     TGRCPLYLLTGEYDFSCTPEDTRLTVEKIPGARFTVMTEIGHFPMAENPAHFRRYILP
                     VLDEIRAAAC"
     gene            complement(2689..3396)
                     /locus_tag="VFX87_14170"
     CDS             complement(2689..3396)
                     /locus_tag="VFX87_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996125.1"
                     /translation="MLAVEGIDVFIQSSHILRQVSLEVRDREVVCLVGRNGAGKTTTL
                     RAIMGYLRPRGGRIVFQGEEIHGLRTHEIAQRGLGFAPEDGGIFTDLTVAENIEIATW
                     TRPGGRPAAERIERAYEVFPVLRSYRHRKGPEMSGGERKMLSIARALALDPHMLVLDE
                     PFEGLSPAIIPAVSRGLAEITQRGHAILLAESNIHHVPEFTTRLYVIERGEIIFAGRP
                     DAVRGDAAVMRVIGGAA"
     gene            complement(3410..4195)
                     /locus_tag="VFX87_14175"
     CDS             complement(3410..4195)
                     /locus_tag="VFX87_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996126.1"
                     /translation="MILEARDVRKLYGNFVALDGVNLAVGAGEFVSIIGPNGAGKSTL
                     INVLTGVLRPTAGTVRFKERDVAGIGPVALTRLGLARSFQLVQIFPDLTVLETLLAPV
                     ISRLGRGTSLFRSLAGDREVQDGALEVAELFGLADKRHVPARQLPQGDKKLLDVASAF
                     ALRPEIILLDEPTSGVSTAEKSAIMEILVTASRRMGLKAIIQVEHDMDIVFGYSDRIV
                     ALHQGKVLADATPAAIRADARVVDMVIGRSHALPSPPSGERAG"
     gene            complement(4192..5148)
                     /locus_tag="VFX87_14180"
     CDS             complement(4192..5148)
                     /locus_tag="VFX87_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996127.1"
                     /translation="MKGRVVLGLALAALLVLPLVLSPYRLLLMLPFMAYGVILLGLNL
                     LFGYTGLVSFGHALFVGIGAYTGAFLTTHAGIRSLEVIVLTAAVLGALTAALVGLLCV
                     RYVKIYFGMLTLAFGMVFYTFLLKFYRLTGGDEGMQVLRPLLLGLNLDAMPKTTYLVG
                     PYYYYCLGVLIVAAAVMWRIVHSPFGLCLRTIRDNPAKAEALGVSVTRYRWYAFVISA
                     VFAAVGGALLGPPTGNVDPTLAYWTHSGSLVFMALLGGFASFFGPVLGAFVFIFLQDA
                     VMSVVPYWRLVFGAILAVIVIFAPGGLMGLFTRRGPVEVSRT"
     gene            complement(5145..6032)
                     /locus_tag="VFX87_14185"
     CDS             complement(5145..6032)
                     /locus_tag="VFX87_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996128.1"
                     /translation="MTANVVNILMSGIFHAAILFLVSAGLQLVFGVQKIFNLACGSFY
                     ALGAYVGVSAVAAFTTAGGPPFLFIVPLALAGILVGSVGLVVERGLLRFVYDRDETFQ
                     LLLTFALVLMMEDAIRLTWGTAPQSTSGHYLLYGQARVLGAVIPVYNLIVIAASVLIA
                     AGLGFMLTRTAFGRIIRAAADNREMAEALGVDMRSVYARVFTLGTALGTLGGALVIPA
                     TAAMSEMGIELIVEAFAVVVIGGLGSMRGALVGALVVGVLRSVAISVYPEIEMLLIYL
                     IVIAVLLLRPRGLFGGAAA"
     gene            complement(6046..7035)
                     /locus_tag="VFX87_14190"
     CDS             complement(6046..7035)
                     /locus_tag="VFX87_14190"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996129.1"
                     /translation="MRRRAFIALLAAGATARPRPAAAQPAGRVYRLGILAPAAPPPPT
                     ERSSAVILIPKALGEMGYAPGRDLVVEVRYAEGQIDRLPDLARELVRLRMDVIVAISA
                     SSVRAVKALTSTIPIVLYGNLDPVAAGLVKSLAKPEGNITGVLIAPAGTLAAKKLELL
                     KEAVPGATRISFLAPADPGIKLQVQETRQAAATLGIRLTVTEVQARDYERAFAALAAD
                     RPDALFVGAHTFFVRDRKRIIALAAKHRLPAVYEWPEQVEDGGLMSYGSSLTGTTRRV
                     AVYVDRLFKGARPADLPIEQPAEFQLVINLRTARALGLTLPPSLVARADRVIE"
BASE COUNT         1142 a         2485 c         2620 g         1009 t
ORIGIN      
        1 gatgacccgg gcgggactga gcccctcggt cgcgggcggg ttcgagtgga gctggcccga
       61 caccccgccc ggattggatc gggtggcgtg gtgggtggcg cgctcggcgg cggagctgct
      121 cacctcgccg gatctagcgg ccgtccgtga gtgcgccggt tatgactgcg gccgcctctt
      181 catggacggg accaagaacc gcagccggcg ctggtgcgac atggcgagct gcgggaaccg
      241 ggccaagggt cggcgccact acgagcgctt gaagtccaca aggtagcgag ccggccgcta
      301 cgcccggacg ggtcccggct ccttcggggc ggtcgcggcc atcccgctac cgcgagccgg
      361 tgagctgctg gcgcgccagc accgcgccgg ccgcgagcgc gcgcaccttc tgattgcaca
      421 tgtagcgcgg ggaccagccg aaaccgcagt cgggattgac gtagagcttc tcggggcggc
      481 agaccgcgag cacccgtcga atgcgggccg ccacgtcctc cgggctctcg gggttgaagc
      541 ccttcacgtc gatgaccccg gcccccagct cgcgatcttg gccgtactcc ttccagaggt
      601 cgagctccga gagctcccgg cccgcgaact cgagcacgag ctgctccgcc ctcgccttca
      661 gcacgccggg gaagtacggc cggtaggagc ggtcgaaccg cgaccggccg aagcggttgc
      721 cgaagcagat gtggaaggcg agcttggcct tcacgccctc ggtggccagg ttgaagaggc
      781 gcgccatctc ctcgccgctc acgttgccgc gcgccggctc gtcgagctgg atgaactcgg
      841 cgcccgcctc cacgaggccg cggagctcgg cgttgatcac ctccgcgaag cgctccgcca
      901 cgtccaccac gcccttgtac atcttgccgg gatggatgcg ggagccgaag gtgagcggcc
      961 ccgcgcaggt ggccttgaca cctcgggtgg tgtgggcctt gaggtactcg aactccttga
     1021 cgataccgag accgcctgcg ggcgcctcga tgcgcccggt ggcctcgtag cgcgtctgct
     1081 ggtcgtagcc ccagggcccg gccttgcgcc gcaccggcag cggctcgatg cccttgatga
     1141 cggcgtagta ggagtccacg tagccgtcga ggcggcgcac ctcaccgtcg gtgatgatgt
     1201 cgagcccggc ccgctccatg tcgcggatgg cggtgtcggc ggcgtcgtcg aacatctcct
     1261 cgaggtcgcc gggccccatg gccccggcct cgtaggcctt cacggcggtg taccaccagc
     1321 cggccttgcc gtgagagccg acgacggtgg acgggatcag cgggagagcg gccatcagcg
     1381 tgtcctcctg acgtgggtga gagcgattat aatgtcccgg cgatgagcgt ttcggcggcc
     1441 gagactcatg cgagcatcct atctctgcct caactggtca ataacgatgc ggggctggtc
     1501 cggcggggtc ggtatctcac cgccaccttc ctggtcgagg ccggcgacgc cgagttcctg
     1561 gtgcgggtgg tggaaggccg gttggccgcg gtcgagcgcg ggcccttcct gatgcgctcg
     1621 tggaccttcg cgctgcgcgc gtccgccgag acctggcagc ggttctggga gcccaccccc
     1681 gctcccgggt atcacgacct cttcgcgatg aagaagctcg gggtggcccg gatcgagggc
     1741 gacctgacgc cgctcatggc ccacctccgc tacgtcaagg acgtggtggc ggcgccccgg
     1801 cggaggccct gaatgaccga gcccatcttc gagccgatca aggggaccta catgcgcctg
     1861 tccctcggcg gccgcccgca ccggctctac ttcgaggagg cggggcaggg gattccgctc
     1921 gtgtgcctgc acaccgcggg cgccgacggc cgccagttcc gccatctgct gaacgacgag
     1981 gcgatcaccc gccacttccg cgtgctggcc ttcgacatgc cctggcacgg caagtccctg
     2041 ccgccggcgg gctggcagga cgaggagtac cgcctgacca cgcgcggcta cgtggatctc
     2101 atcctggcgt tctgcgccgc actcggcctc gagcggccgg tggtcctcgg ctgctcgatc
     2161 ggcggcaaga tcgtcctcga gctggcgcgc acgcatcccg agtgcttccg cgccctgatc
     2221 gggctcgagt ccgccgccta tcagccgccc tggtacgacg acaccggctg gctccaccgc
     2281 ccggacgtgc acggcggcga gctctgcggg gcgctcatgt cggggctcat cgccccgcag
     2341 agcccggacg agacgcgctg ggagacgctc tggggctaca tgcagggcgg gccgggtgtg
     2401 ttcaagggag atctccactt ttaccgagta gacggcgacc tccgcgaccg ggtcggccag
     2461 atcgacaccg ggcgctgccc gctctacctg ctgaccggtg agtacgactt ctcgtgcacg
     2521 ccggaggaca cccggctcac cgtcgagaag atcccgggcg cgcgcttcac ggtcatgacc
     2581 gagatcggcc atttcccgat ggccgagaac ccggcgcatt tccgccgcta catcctgccc
     2641 gtcctcgacg agatccgcgc cgccgcgtgc tgagcggcgc cgcggccgtc acgccgcgcc
     2701 gccgatcacc cgcatcacgg ccgcgtcccc gcgcacggcg tccgggcgcc ccgcgaagat
     2761 gatctccccg cgctcaatca cgtagaggcg cgtggtgaac tcgggcacgt ggtggatatt
     2821 ggactcggcg agcaggatcg cgtgcccgcg ctgggtgatc tcggcgaggc cgcggctgac
     2881 cgcgggaatg atggcggggg agaggccctc gaagggctcg tcgagcacca gcatgtgggg
     2941 atcgagcgcg agggcccgcg cgatcgagag catcttccgc tcgccgccgc tcatctcggg
     3001 gcccttgcgg tggcggtagc tccgcagcac cgggaacacc tcgtaggcgc gctcgatgcg
     3061 ctcggccgcg gggcggccgc ccggccgcgt ccaggtggcg atctcgatgt tctccgccac
     3121 cgtgaggtcg gtgaagatcc cgccgtcctc gggcgcgaac ccgaggccgc gctgagcgat
     3181 ctcgtgggtg cggagcccgt ggatctcctc gccctggaag acgatgcggc cgccccgtgg
     3241 gcggaggtag cccatgatcg cgcgcagcgt ggtggtcttg cccgcgccgt tgcggcccac
     3301 caggcagacc acctcccggt cccggacctc gagggagacc tgtcgcagga tgtggctgct
     3361 ctggatgaac acgtcgatgc cctccaccgc gagcatgacg ggacctccct caccctgccc
     3421 tctcccccga agggggagag gggagagcgt ggctccggcc gatgaccatg tccaccacgc
     3481 gcgcgtcggc gcggatcgcg gcgggcgtcg cgtcggccag gaccttcccc tggtggagcg
     3541 cgacgatgcg gtccgagtaa ccgaagacga tatccatgtc gtgctcgacc tggatgatcg
     3601 ccttgagccc catgcgccgg gaggcggtga ccaggatctc catgatggcc gacttctcgg
     3661 ccgtgctcac gccgctggtc ggctcgtcca acaggatgat ctcgggcctg agcgcgaagg
     3721 cggaggccac gtcgaggagc ttcttgtcgc cctgggggag ctggcgcgcc ggcacgtggc
     3781 gcttgtcggc cagcccgaac agctcggcga cctcgagcgc gccgtcctgc acctcgcggt
     3841 cgccggccag cgagcggaag agcgacgtgc cccggccgag ccgggagatg accggggcca
     3901 gcagggtctc gagcacggtg aggtccggga agatctgcac gagctggaag ctccgggcga
     3961 ggccgagccg ggtcagggcc accgggccga tgccggccac gtcgcgctcc ttgaagcgca
     4021 ccgtccccgc ggtgggccgc agcacgccgg tgagcacgtt gatgagcgtg gacttgcccg
     4081 cgccgttggg gccgatgatc gagacgaatt cgcccgcgcc caccgcgagg ttcaccccgt
     4141 cgagcgcaac gaagttgccg tagagcttgc ggacgtcgcg cgcctccagg atcacgtccg
     4201 cgagacctcg accggcccgc ggcgggtgaa gaggcccatg aggccgccgg gagcgaagat
     4261 cacgatgacg gccaggatgg cgccgaacac gaggcgccag tacggcacca ccgacatcac
     4321 cgcgtcctgc aggaagatga agacgaaggc gcccagcacc gggccgaaga agctggcgaa
     4381 gccgcccagc agcgccatga acacgagact gcccgagtgg gtccagtagg ccagcgtggg
     4441 gtccacgttg ccggtcggcg gaccgagcag cgcgccgccg acggccgcga acaccgccga
     4501 gatcacgaag gcgtaccagc gataacgcgt cacgctcacc ccgagcgctt ccgccttcgc
     4561 cggattgtcg cggatcgtgc gcaggcagag gccgaagggc gagtgcacga tccgccacat
     4621 cacggcggcg gccacgatca ggacgcccag gcagtagtag tagtaggggc ccaccaggta
     4681 ggtggtcttg ggcatcgcgt ccaggttgag cccgagcagg agcggccgca gcacctgcat
     4741 gccctcgtcg cctccggtga gccggtagaa cttgagcagg aatgtgtaga agaccatgcc
     4801 gaaggccaat gtgagcatgc cgaagtagat cttcacgtag cgcacgcaga ggaggcccac
     4861 gagggcggcg gtgagcgcgc ccaggactgc ggcggtgagc acgatcacct cgagcgagcg
     4921 gatgcccgcg tgggtggtga ggaaggcacc ggtataggcg ccgatgccca cgaagagcgc
     4981 gtggccgaag gagacgaggc cggtgtagcc gaagagcagg ttgagcccga gcaggatcac
     5041 gccgtaggcc atgaacggca gcatgaggag caggcggtag gggctcagca cgagcggaag
     5101 gacgagcagc gccgccaggg ccaggcccag gaccacgcgt cccttcacgc ggccgctccc
     5161 ccgaagagcc cgcgcgggcg cagcagcagc accgcgatca cgatcaggta gatcagcagc
     5221 atctcgatct cgggatacac gctgatcgcg accgagcgga gcacgcccac caccagggca
     5281 cccaccagcg cgccgcgcat gctgccgagc ccgccgatca ccaccaccgc gaaggcctcg
     5341 acgatcagct cgatgcccat ctcgctcatc gccgcagtgg ccgggatcac gagcgcgccc
     5401 ccgagggtgc cgagcgcggt gccgagggtg aagacccgcg cgtagacgct gcgcatgtcc
     5461 accccgaggg cctcggccat ctcgcggttg tcggccgcgg cccggatgat gcggccgaac
     5521 gcggtgcggg tcagcatgaa gccgaggccc gccgcgatca gtacgctcgc cgcgatgacg
     5581 atcaagttat agacgggtat taccgcgccc agcacccggg cctggccgta gagcaggtag
     5641 tgccccgacg tggactgggg cgcggtgccc caggtgagac ggatcgcgtc ctccatcatg
     5701 aggacgagcg cgaacgtgag caggagctgg aaggtctcgt cgcggtcgta gacgaagcgg
     5761 agcagcccgc gctccaccac gaggcccacc gagccgacca ggatgcccgc gagggcgagc
     5821 ggcacgatga agaggaacgg gggcccaccg gcggtcgtga acgcggcgac cgcggacacg
     5881 cccacgtagg cgccgagcgc gtagaacgac ccgcacgcga ggttgaagat cttctggacg
     5941 ccgaagacga gctgcagtcc cgccgagacc aggaacagga tcgcggcgtg gaagatgccg
     6001 ctcatcagga tgttgacgac gttcgcggtc acgcgggagg gccggctact cgatgacgcg
     6061 atcggcccgt gccacgagcg atggcgggag tgtgagcccg agggcgcggg cggtccggag
     6121 attgatcacg agctggaact cggcgggctg ctcgatcggc aggtcggcgg gccgggcccc
     6181 cttgaagagg cgatccacgt agaccgcgac ccggcgggtc gtcccggtga ggctgctgcc
     6241 gtacgacatc aggcccccgt cctccacctg ctcgggccac tcgtagaccg cgggcaggcg
     6301 gtgcttcgcg gcgagcgcga tgatccgctt ccggtcgcgc acgaagaagg tgtgcgcgcc
     6361 gacgaagagc gcgtccggcc gatcggccgc cagcgcggcg aaggcccgct cgtagtcgcg
     6421 ggcctggact tcggtgacgg tcagcctgat gccgagcgtc gccgcggcct gccgcgtctc
     6481 ctgcacctgg agcttgatgc cgggatccgc gggcgcgagg aaggagatgc gggtggcccc
     6541 ggggaccgct tccttgagca attcgagctt cttggccgcc agggttccgg cgggtgcgat
     6601 caagactccg gtgatgttgc cctcgggctt ggcgaggctc tttacgagcc cggccgcgac
     6661 cgggtcgaga tttccgtaca ggacgatcgg gatcgtggac gtcagtgcct tcaccgcccg
     6721 gacggaggac gcgctgatcg cgacgatgac gtccatgcgg agccggacca gctcgcgcgc
     6781 cagatcgggg agccggtcga tctggccctc cgcatagcgc acctcgacca cgagatcacg
     6841 gcccggcgca tagcccatct cgcccagggc cttcgggatc aagatcacgg cactgctccg
     6901 ctcggtcggc ggcggcgggg ccgcgggcgc gaggatgccc aggcggtaga cgcgccccgc
     6961 cggctgcgcg gcggcggggc gcggccgggc ggtggcgcca gcggcgagga gggcgatgaa
     7021 ggcgcgtcgg cgcatcgggg tcacggcacg gccccctcac cctgccctct cccccgaggg
     7081 gagagggaga gccgacaccg gctggagcgg gaaccctctc ctccgtgagg gggagagggc
     7141 agggtgaggg ggagcgggat cagatcttcc agctcgcgat ccagtccatc aacttggcgc
     7201 cggccggctt catgccctgc ttggtggaga cgacctcgag cgggttcacg gtcacg
//
LOCUS       DASUFA010000390         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1685584, whole genome shotgun sequence.
ACCESSION   DASUFA010000390 DASUFA010000000
VERSION     DASUFA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685584"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFX87_14195"
     CDS             <1..651
                     /locus_tag="VFX87_14195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4996130.1"
                     /translation="VEEGVGVRGLAVEGGRVVGVECGDGAREACDGAIVTAGAWSATL
                     LAGIGVALPIETRPLQMLLTAPGPPALRPVLGAFDRKLSLKQLDDGAYLIGGGWPARV
                     TDHAGNRYEMLEASVRASLETARAVFPPVGACGLARSWAGLEAFTPDDLPILGPVPGV
                     RGLLVAAGFSGHGFALSPMIGDILARLALGQDALESLWKGLRFDRGQVLHSNNVGM"
     gene            complement(671..1945)
                     /locus_tag="VFX87_14200"
     CDS             complement(671..1945)
                     /locus_tag="VFX87_14200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4996131.1"
                     /translation="MSFSEFQLHPDLLRGVESLGFQAPTAIQRDAIPPAMAGRDVLAC
                     AVTGSGKTAAFLLPILQKLIGLPRGTTRALIIAPTRELAAQIDEHRGQLARHTRVSGA
                     AIFGGVGMGPQESALRRGVDIIVATPGRLLDHMRMPYTRLDGLQVLVLDEADRMLDMG
                     FLPDIQRILKQLPARRQTLLLSATMPGPIAALAKQILHDPVAIQIERPSAPAVGITHT
                     VYQVPQELKSALLVELLKQPGTRSVLAFTRTKHRANRLADYLARHGVKADRIHGNRSQ
                     AQRTQALAGFKDGRFQVLVATDIAARGIDIDALSHVVNFDVPGMPDEYIHRVGRTARA
                     EAVGDALTLVSPDEEGDLRGIERAVGSRFTRAQLPGFDYTKRPADKLEIPLAERIAQI
                     RARKAEDRQRARANAERRAAHGTRPAQLTRGR"
     gene            complement(2110..2331)
                     /locus_tag="VFX87_14205"
     CDS             complement(2110..2331)
                     /locus_tag="VFX87_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996132.1"
                     /translation="MPRTPTKSYNCSVVSETVSISLRRRQSLGGHGKLFVRCSETECQ
                     YIDTNEPPCPLTLQLFEAEIAERMAQRTE"
     gene            complement(2396..3103)
                     /locus_tag="VFX87_14210"
     CDS             complement(2396..3103)
                     /locus_tag="VFX87_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996133.1"
                     /translation="MLRISGLSVSYGGLHALEAVSLEVNEGEFVAIVGPNGAGKTTLL
                     KAISGSVRAGGGRLEYRGRDMAPLAPHQRAALGIAHVPEGRRIFPTLTVLENLELGSY
                     RRAARGRRAEGLESVMALFPVLRERRGQAAGSLSGGEQQMLALGRGLMAVPELLLLDE
                     PSQGLAPRIVEQIFETIDRIRRERRLTILLVEQRVVEALELCDRGYVLETGRVALEGT
                     HTALLADPRVQRAYLGA"
     gene            complement(3081..4847)
                     /locus_tag="VFX87_14215"
     CDS             complement(3081..4847)
                     /locus_tag="VFX87_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX4996134.1"
                     /translation="MTRDRGWLGIAAVVAIGLVLSLALTERYHHRILTLVFLWATMGL
                     AWNIISGYAGQISFGHQAFFGIGAYVTVLLAAKLHLTPWLGMLGGAGVAVLAALLIGT
                     PTFRLAGIYFGLATLAYPLIFRILMDFLGYQEVAIPMVRDHPVLFMQFEEPRTFDLLA
                     LALLAATLVLSRLIEASRLGYSLRAIKENEQAAEAMGVDAFRCKMTAYVLSAAPAALA
                     GAIYAHAILFVVTPDGVFGVIAIVQTLTVCLVGGVGTLWGPVIGAAIMIPISEILDAT
                     AGDRFPGIQGVVYGATLMAIMMWAPEGLFWRLRRRPASVARATPVAATAATAETPAAA
                     PGPVQLEVRGISKAFIGLQALADVSFDVREGEILGIIGPNGAGKTTLFNVLNGFLLPD
                     SGDVRWLDAPITGLRPHLVCRRGIGRTFQVVRFFPHLTVLENVMVGAFAHGDAPGAAR
                     ERARASLDRVSLAGQADTLPAGLTTLDLRLMELARCLATSPRLVLLDEPLAGLSSDGV
                     EMMAVMIRRTRAAGVTVVIIEHTIQALVKLADRLVVLDHGRRLAEGAPGDVTRNPAVI
                     EAYLGKRWLARHGPDHAANLRP"
     gene            complement(4849..>5068)
                     /locus_tag="VFX87_14220"
     CDS             complement(4849..>5068)
                     /locus_tag="VFX87_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996135.1"
                     /translation="LVAIGDAFVVVVVGGLGSIPGAFVGGIVIGLVQSLTLLLLPLQL
                     QNVGVFVAFLIVLYVRPQGLFGRRARSV"
BASE COUNT          747 a         1723 c         1916 g          682 t
ORIGIN      
        1 gtcgaggagg gcgtcggcgt gcgcgggctc gcggtcgagg gcggccgggt ggtgggtgtc
       61 gagtgcggcg acggcgcgcg cgaggcctgc gacggggcga tcgtgacggc gggcgcgtgg
      121 agcgcgacgc tcctcgcggg gatcggcgtg gcgctgccga tcgagacgcg cccgctccag
      181 atgctgctca ccgcgccggg cccgcccgcg ctccggccgg tgctgggcgc cttcgaccgc
      241 aagctgagcc tgaagcagct cgacgacggc gcctacctga tcggcggggg ctggcccgcg
      301 cgcgtcacgg accacgcggg caatcgctac gagatgctgg aggcgagcgt gcgcgcgagc
      361 ctcgaaacgg cgcgcgcggt ctttcccccg gtcggcgcgt gcgggctcgc gcggagctgg
      421 gcggggctcg aggccttcac gccggacgac ctgcccatcc tgggtcccgt ccccggtgtc
      481 cgcggattgc tggtcgccgc cggcttctcg ggccacggct tcgcgctgag cccgatgatc
      541 ggcgacatcc tcgcacgcct tgccctgggc caggatgcgc tcgaatcact ctggaagggt
      601 ctgcgcttcg atcgtggtca ggtcttacat tccaacaatg tgggaatgta agacctgacc
      661 ccgctagtga ctagcggccg cgggtgagct gggcggggcg ggtgccgtgg gccgcgcgcc
      721 gctcggcgtt ggcgcgggcg cgctgccggt cttcggcctt gcgggcgcgg atctgcgcga
      781 tgcgctcggc cagcgggatc tccagcttgt cggccggccg cttggtgtag tcaaagccgg
      841 gtagctgcgc gcgcgtgaag cgcgagccca ccgcccgctc gatgccgcgc aggtcgccct
      901 cctcgtccgg cgagaccagg gtgagcgcgt cgcccaccgc ctcggcccgc gcggtgcggc
      961 ccacccggtg gatgtactcg tcgggcatgc ccggcacgtc gaagttcacc acgtggctga
     1021 gcgcgtcgat gtcgatgccg cgggccgcga tgtcggtggc cacgagcacc tggaagcgcc
     1081 cgtccttgaa gccggccagc gcctgcgtcc gctgcgcctg gctgcggttg ccgtggatgc
     1141 gatcggcctt gaccccgtgg cgggccaggt agtcggccag ccggttggcc cggtgcttgg
     1201 tgcgcgtgaa ggcgagcacg ctgcgcgtgc cgggctgctt gagcagctcg accagcaacg
     1261 ccgacttcag ctcctgcggc acctggtaca cggtgtgggt gatgccgacc gcgggcgcgg
     1321 acggccgctc gatctggatg gccaccggat cgtggaggat ctgcttcgcg agcgccgcga
     1381 tggggccggg catggtcgcg gagagcagca gcgtctgccg gcgcgccggg agctgcttca
     1441 ggatccgctg gatgtccggc aggaagccca tgtccagcat gcggtcggcc tcgtccagca
     1501 ccagcacctg gaggccgtcg agcctggtat acggcatgcg catgtggtcg agcaggcggc
     1561 ccggcgtggc gacgatgata tccaccccgc ggcggagcgc cgactcctgc gggcccatgc
     1621 ccacgccgcc gaagatggcc gcgccgctca cccgcgtgtg acgggcgagc tggccgcggt
     1681 gctcgtcgat ctgggccgcg agctcgcggg tgggggcgat gatcaaggcg cgcgtggtgc
     1741 cacgggggag cccgatcagc ttctgcagaa tgggcagcag gaacgccgcg gtcttgccgc
     1801 tgccggtcac ggcgcaggcg agcacgtcgc gcccggccat cgcgggggga atggcgtcac
     1861 gctggatggc ggtcggcgcc tggaaaccca gcgactcgac gccacggagc agatcgggat
     1921 gaagctggaa ttcggagaag gacatggagc gagtaacctc ttgcgggcac agccgggccc
     1981 gtacagtctt ccgcggattt cttggcgcgc cccacgatgc ggaagaggac gggaaacgcg
     2041 gtctgcgatg ttgcagtggt tacagcatac acgcggccgg cggccggcgc gaagaaataa
     2101 cgctgggcgt tactcggtcc gctgggccat ccgctccgcg atctccgcct cgaagagctg
     2161 cagcgtcagc gggcagggcg gctcgttggt gtcgatgtac tggcactcgg tctcgctgca
     2221 gcggacgaag agcttgccgt ggccgccgag cgactggcgg cggcgcagcg agatggagac
     2281 cgtttccgag acgacgctgc agttgtacga cttcgtggga gtccggggca cggggatcct
     2341 ttcctcgcgg tcattgcgac cgaggactcc agtatacacg gtctcgacgg agtaatcagg
     2401 ccccgagata ggcgcgctgc acgcgcgggt cggcgagcag cgcggtgtgc gtgccctcga
     2461 gggcgacgcg acccgtttcc agcacgtagc cgcggtcgca gagctcgagc gcctcgacca
     2521 cccgctgttc taccaggaga atcgtgagcc gccgctcgcg gcggatgcgg tcgatggtct
     2581 cgaagatctg ctcgacgatg cgcggggcca ggccctgcga gggctcgtcg agcagcagca
     2641 gctccggcac cgccatcagc ccgcggccca gcgccagcat ctgctgctcg ccgcccgaca
     2701 gcgagccggc ggcctgcccg cgccgctcgc ggagcaccgg gaagagggcc atcaccgact
     2761 cgagcccctc cgcgcggcgg ccgcgcgcgg cccgccggta ggagccgagc tccaggttct
     2821 ccagcacggt gagcgtcggg aagatgcgcc gaccctccgg tacgtgcgcg atgccgagcg
     2881 ccgcgcgctg gtgcggcgcg agcggggcca tgtcccgccc gcggtactcg aggcgtccgc
     2941 cgcccgcccg caccgagccc gagatggcct tgagcagggt ggtcttgccc gcgccattcg
     3001 ggcccacgat ggccacgaac tcgccctcgt tgacctcgag cgagacggcc tcgagggcat
     3061 ggaggccccc gtaggagacg ctaaggccgg agattcgcag catggtcggg tccgtggcgg
     3121 gcgagccagc gcttgcccag gtaggcctcg atcaccgccg ggttgcgggt cacgtcgccc
     3181 ggcgcgccct cggcgagccg ccggccatgg tccagcacca ccagccgatc ggcgagcttc
     3241 acgagggcct ggatggtgtg ctcgatgatc accaccgtca cgccggcggc ccgggtgcgc
     3301 cggatcatca ccgccatcat ctcgacgccg tcggacgaca ggccggcgag cggctcgtcc
     3361 agcagcacga gccggggcga cgtggccagg cagcgcgcca gctccatgag ccgcaggtcg
     3421 agcgtggtga gcccggcggg cagcgtgtcc gcctgcccgg cgaggctcac gcggtcgagc
     3481 gaggcccgtg cgcgctcccg cgcggcgccc ggcgcgtcgc cgtgcgcgaa agcgcccacc
     3541 atgacgttct ccagcacggt gaggtgcggg aagaagcgca ccacttggaa ggtgcgaccg
     3601 atgccgcgcc ggcagaccag gtgggggcgc aggccggtga tcggtgcgtc gagccagcgc
     3661 acgtcgccgc tgtcgggcag caggaagccg ttgagcacgt tgaagagcgt ggtcttcccc
     3721 gcgccgttcg ggccgatgat cccgaggatc tcgccctcgc gcacgtcgaa gctcacgtcg
     3781 gccagcgcct gcaggccgat gaacgccttg ctgatcccgc ggacctcgag ctgcacgggc
     3841 ccgggcgcgg cggcgggcgt ctccgcggtc gcggcggtgg cggccaccgg ggtcgcgcgc
     3901 gcgacggacg cggggcgccg gcgcaggcgc cagaacaggc cctcgggtgc ccacatcatg
     3961 atggccatca gcgtcgcgcc gtacacgacg ccctggatcc cggggaagcg gtcgcccgcg
     4021 gtggcgtcga ggatctcgct gatcgggatc atgatggcgg ccccgatgac cgggccccag
     4081 agcgtaccca ccccgccgac caggcagacc gtgagggtct gcacgatcgc gatcacaccg
     4141 aagacgccgt ccggggtgac cacgaacagg atggcgtggg cgtagatcgc ccccgcgagg
     4201 gcggcgggcg ccgcgctcaa cacgtaggcg gtcatcttgc agcggaaggc gtccacgccc
     4261 atggcctcgg cggcctgttc gttctccttg atcgcgcgca gcgagtagcc gaggcgtgag
     4321 gcttcgatca ggcgcgagag cacgagggtg gcggcgagca gggccagggc cagcagatcg
     4381 aacgtccgcg gctcctcgaa ctgcatgaac agcaccgggt ggtcgcgcac catcgggatc
     4441 gcgacttcct ggtagccgag gaagtccatg aggatccgga agatcagcgg ataggcgagc
     4501 gtggcgagcc caaaatagat gccggccagc cggaaggtgg gcgtgccgat gagcagggcg
     4561 gcgagcaccg cgacgccggc gccgcccagc atgccgagcc acggggtgag atggagcttg
     4621 gcggccagca gcacggtgac gtaggccccg atgccgaaga aggcctggtg gccgaaggag
     4681 atctggcccg cgtagccgct gatgatgttc caggcgagcc ccatggtggc ccacaggaac
     4741 accagcgtga gaatgcggtg gtggtagcgc tcggtcaggg ccagggagag cacgagcccg
     4801 atcgcgacca ccgccgcgat gccgagccag ccgcggtcgc gggtcacgct agacgctgcg
     4861 ggcccggcgg ccgaagaggc cctgcggccg gacgtagagg acgatcagga acgccacgaa
     4921 cacgcccacg ttctggagct gcagcggcag gagcagcagg gtcagggact ggacgaggcc
     4981 gatcacgatc ccgcccacga aggcccccgg gatgctgccg agcccgccca ccaccaccac
     5041 cacgaaggcg tcgccgatgg cgacgagg
//
LOCUS       DASUFA010000391         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1350427, whole genome shotgun sequence.
ACCESSION   DASUFA010000391 DASUFA010000000
VERSION     DASUFA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350427"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1805)
                     /gene="ggt"
                     /locus_tag="VFX87_14225"
     CDS             complement(138..1805)
                     /gene="ggt"
                     /locus_tag="VFX87_14225"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4996136.1"
                     /translation="MTEKRPATASRGMVVTNHPLGSSAGAEMLAAGGNAVDAAIGALF
                     ALTVVEPMMVGIFGAGMTHLRLADGRHLVIDNYTTAPAASRPEMFKPISDTWPDYLRA
                     EGDVNLVGLLSVGVPGTLKAWAEAIARFGRLDLETVMQPAIRHAERGFRATPYLVAAV
                     TETGPDLARFPETARTFLPGGAPIKAGHLIVMPEYAATLRLIAAKGPGALYGGDLGAT
                     VADYMRRDGGIITLDDLARYQTIERQPIRGRYRGFEITGPPPPTAGGLHLVEMLNILE
                     GFDPRGLGFGTADYFHLIAEVLKIGFADRNACTGDPAFVDIPVERLISKEHADTRRRQ
                     IRLDRAGEYGATVAVTGGVAAGSAHTTHVTTADADGNVVAMTQTINNLFGSKATVPGT
                     GILLNNTMALFDPHPGTPASIAPGKRVTSSMAPTIVLRDGKSTWALGLPGGVRIFTSV
                     FQAVVNLIDHGMTLQEAVEAPRIWSQGQELEMEQDIPAAVRDAVAARGHRVVAVPGVA
                     GGMNAVAFAGDGTLEGAACWRADGTPIGVSGGLARAGSRFRPDVIRR"
     gene            complement(1813..2787)
                     /locus_tag="VFX87_14230"
     CDS             complement(1813..2787)
                     /locus_tag="VFX87_14230"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX4996137.1"
                     /translation="MLRTRICELLGIEHPIVLGGMGTGTSPALVAAVSETGGLGVLGA
                     SQQDPAQVARDAAAIRALTGKPFGLNLLLFRERPGQYDGILAARPRIVSTAWPTLEQD
                     LADYVARAHAIGALSMHMISTVAEARVAARSGVDVIVAQGTEGGGHVGVMGTMPLVPM
                     VVSAVAPVPVLAAGGVADGRGLAAALALGADGVLLGTRFLASDEAPIAKGFKQAILES
                     DGHDTLVTDIPDVAHGQVWPGAYVRVRRNRFIEEWIGRDNELRRHRVEVSAQLREAAK
                     AGDPDRSAIMIGQTAGLIDRVEPAADLVRQISRDAEAILRDRLPRFLV"
     gene            complement(2863..>3657)
                     /locus_tag="VFX87_14235"
     CDS             complement(2863..>3657)
                     /locus_tag="VFX87_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4996138.1"
                     /translation="DMIELLDALGREKAVWVGHDWGSPVVWSLASHHPERCWGVGNLC
                     VPYLKAGFTVRNLIPLVDRAVYPEAQYPAGQWDYMLFYEESFEKACAAFDADPRNTVK
                     ALFRKGSPGGKGKPSRTAQTRKDGGWFGGTGRAPDVPMDADVVTAEELDMYAAGLARN
                     GFFGPDSWYMNAERNLEYAGRARDGGTLSLPVLFLHGEYDYTCETVGSRLAEPMRRDC
                     RNLTEVIVPSGHWMAQEKPRAVNAALARWLATELPQVWPIQPHPRA"
BASE COUNT          497 a         1339 c         1290 g          531 t
ORIGIN      
        1 gcgggcaccc tggtcagctc ggcgaacgtg gaaggctaca cgcgcaaggg cgtccgctgc
       61 atcatgaccg gcttcttccc gtggatccag gccggcgcga aggacctgat ggaccgcgcc
      121 gcggcgggag cgaagggcta gcgccggatc acatccgggc ggaagcggct ccctgcccgc
      181 gcaagcccgc cgctcacacc gatcggcgtg ccgtcggcgc gccagcaggc cgcgccttcg
      241 agcgtgccgt ccccggcgaa ggccaccgcg ttcatgccgc ccgcgacgcc cgggacggcc
      301 accacgcggt gtccgcgcgc ggcgaccgcg tcgcgtaccg ccgcgggaat atcctgctcc
      361 atctcgagct cctggccctg gctccagatc cgcggcgcct ccacggcttc ctgcagcgtc
      421 atgccgtggt cgatcaggtt gaccaccgcc tggaacacgg acgtgaagat gcggacaccc
      481 cccggcaggc cgagcgccca cgtggacttg ccgtcgcgca gcacgatggt cggcgccatg
      541 ctactggtca cccgcttgcc gggcgcgatc gaggcgggcg tgcccgggtg cgggtcgaag
      601 agcgccatgg tgttgttgag caggatcccg gtgccgggca cggtggcctt ggagccgaac
      661 aggttgttga tggtctgggt catcgcgacc acgttgccgt cggcgtcggc ggtcgtgacg
      721 tgcgtggtgt gcgcggagcc ggccgcgacg ccccccgtga ccgccacagt cgcgccgtac
      781 tcccccgccc gatccaggcg gatctgccgg cgccgcgtat ccgcgtgctc cttcgagatc
      841 aggcgctcga ccgggatgtc cacgaaggcc ggatcgccgg tgcacgcgtt gcggtctgcg
      901 aagccgatct tgagcacctc ggcgatcagg tggaagtagt ccgcggtgcc gaagcccaga
      961 ccgcgcggat cgaagccttc caggatgttc agcatctcga ccaggtggag cccgcccgcg
     1021 gtcggcggcg ggggccccgt gatctcgaag ccgcggtagc ggccgcggat gggctgccgc
     1081 tcgatcgtct ggtagcgcgc caggtcgtcc agggtgatga ttccgccgtc tcggcgcatg
     1141 tagtcggcca ccgtcgcgcc caggtcgccc ccgtagagcg cgccggggcc cttggccgcg
     1201 atcaggcgca gggtcgccgc gtactccggc atgacgatca ggtggcccgc cttgatgggg
     1261 gccccgcctg gcaggaacgt ccgcgcggtc tcggggaagc gtgcgagatc cggcccggtc
     1321 tccgtcaccg ccgctaccag gtacggcgtc gcccggaagc cgcgctcggc gtggcggatc
     1381 gcgggctgca tcacggtctc caggtcgagg cgcccgaagc gcgcgatcgc ctccgcccag
     1441 gccttgagcg tgccgggcac gcccaccgag aggaggccga ccaggttgac gtcgccttcg
     1501 gctcgcaggt agtcgggcca ggtgtcggag atgggcttga acatctcggg ccgcgaggcc
     1561 gccggcgccg tcgtgtagtt gtcgatgacg aggtgccgac cgtcggccag ccgcaggtgc
     1621 gtcatgcccg cgccgaagat gccgaccatc atcggctcca ccacggtcag cgcgaagagc
     1681 gcgccgatgg ccgcgtccac cgcgttaccg ccggcggcca gcatctccgc gcccgccgac
     1741 gagccgagcg gatgattggt gaccaccatg ccccgtgacg ccgtcgccgg tcgcttctcg
     1801 gtcatggcgg gctcagacga ggaagcgtgg caggcggtcg cgcaggatgg cctcggcgtc
     1861 tcggctgatc tgcctgacca ggtcggcggc cggctcgacg cgatcgatga ggccggcggt
     1921 ctggccgatc atgatcgcgc tccggtccgg atcgcccgcc ttggcggcct cgcgaagctg
     1981 ggcgctcacc tcgacccgat gccgccgcag ctcgttgtcg cggccgatcc actcctcgat
     2041 gaagcgattg cgtcgcacgc gcacgtaggc gccgggccag acctgcccat gggcaacgtc
     2101 gggaatgtcg gtcaccaggg tgtcgtggcc gtcgctctcg aggatcgcct gcttgaagcc
     2161 cttggcgatg ggggcctcgt cgctggccag gaagcgcgtc cccagcagga cgccgtccgc
     2221 gccaagcgcc agcgccgcgg ccagcccgcg gccgtccgcg accccgccgg cggccagcac
     2281 cgggaccggg gcgacggcgc tgaccaccat ggggacgaga ggcatcgtcc ccatgacccc
     2341 gacatgaccg ccgccctcgg tgccctgcgc cacgatcacg tcgaccccgc tgcgcgccgc
     2401 caccctcgcc tcggccaccg tcgagatcat atgcatggac agggcgccga tcgcgtgcgc
     2461 ccgcgcgaca tagtcggcca gatcttgctc cagcgtcggc caggcggtcg agacgattcg
     2521 aggccgcgcg gccaggatcc cgtcgtactg gccgggccgc tccctgaaca ggagcaggtt
     2581 caggccgaag ggcttgccgg tcagcgcgcg gatcgccgcc gcatcgcggg ccacttgcgc
     2641 cgggtcctgc tgtgacgcgc ccagcacgcc caggccaccc gtctcggaca ccgcggccac
     2701 cagcgcgggc gacgtgccgg tccccatgcc gcccagaacg atcggatgct cgataccgag
     2761 cagctcgcag atgcgtgtac gcagcatggt cgcttctcct tctcaggctc gcgggcgcgg
     2821 gctcaggctc gcgggcgcgg gctcaggctc gcgggcgcgg gctcaagctc gcgggtgcgg
     2881 ctggatcggc cacacctgcg gcagctcggt agccagccac ctcgccaggg ccgcgttcac
     2941 cgcgcgcggc ttctcctgcg ccatccagtg tccggaaggc acgatcacct cggtgaggtt
     3001 ccggcagtcg cgccgcatgg gctcggccag ccgggagccg acggtctcgc aggtgtagtc
     3061 gtactcgccg tgcaggaaca ggacgggcag gctgagcgtg ccgccgtccc tggcccggcc
     3121 cgcgtactcc aggttgcgct cggcgttcat gtaccaggag tccgggccga agaagccgtt
     3181 gcgcgcgagg ccggccgcgt acatgtcgag ctcctccgcg gtcaccacgt cggcatccat
     3241 cggcacgtcc ggagcccgcc cggtgccgcc gaaccagccg ccgtccttgc gggtctgcgc
     3301 ggtgcgggac ggcttgccct tgccgccggg gctccctttc cggaagagcg ccttgacggt
     3361 attgcgcggg tcggcgtcga acgcggcgca cgccttctcg aagctctcct cgtagaagag
     3421 catgtagtcc cactggcccg ccgggtactg cgcttccgga tacaccgccc ggtccacgag
     3481 gggaatcagg ttgcgcacgg tgaagcccgc cttgagatag ggcacgcaga ggttgcccac
     3541 gccccagcat cgctccgggt ggtggctcgc caggctccag acgaccgggc tgccccaatc
     3601 gtggccgacc cacaccgcct tctcccggcc cagcgcgtcc agcagctcga tcatgtc
//
LOCUS       DASUFA010000392         5541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_780333, whole genome shotgun sequence.
ACCESSION   DASUFA010000392 DASUFA010000000
VERSION     DASUFA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780333"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFX87_14240"
     CDS             <1..395
                     /locus_tag="VFX87_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX4996139.1"
                     /translation="GALVTGAQLNEDGTARMDFDLPDADNDRLRALEPAINDVIRQDL
                     PVRYAYVTESEAVSERGLIRNRSVAPPPTADGTIRVVEIVGLDRQACGGTHLASTAGS
                     RQLRVLKIDNKGRHNRRVKIGLVETSEG"
     gene            complement(427..807)
                     /locus_tag="VFX87_14245"
     CDS             complement(427..807)
                     /locus_tag="VFX87_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX4996140.1"
                     /translation="MTERVINLKSFFEEARAGRLTGIRCGRCGELAIPPKEFCAGCQQ
                     RDWQPVPLTGAGAIVSYTVIRVAPRGRGGEVPYAVAVVKLDEGVSLLGRLVDLPLDAL
                     ASGLRVRFRPIELAGETVSGFGPA"
     gene            complement(800..1954)
                     /locus_tag="VFX87_14250"
     CDS             complement(800..1954)
                     /locus_tag="VFX87_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX4996141.1"
                     /translation="MRRVAVIGVGVTKFGKHDRTSAELFAAAAADAITDAGIAPNAVQ
                     AMYFGNVTGGETERQLHMGPLAATLLGLPTLPTTRFENACATSHVAFRHAVMEIASGV
                     SDVVLVGGAERVLNVPTESATEFFAYCSDASLEQPAGLTFPGVFALIARAHMDKYGTT
                     EEQMAHVAVKNHRHGVLNPKAQFQKEITLETVLKSAYVADPLKLFDCCPFTDGGAALV
                     LASEEVARRHRRPIWVLGTHAATDTMFVHEKRDLSRVTATERAAQGAYRQAGKTPTDV
                     DVVELHDCFTIAEIVATEGLGFFEPGTGGLAAEKGWTSLGGKLPVNPSGGLKAKGHPI
                     GATGAAQIAEIITQLRGEADRRQVDGARTGLTHTLGGNTATVVVSLFGRD"
     gene            2064..3026
                     /locus_tag="VFX87_14255"
     CDS             2064..3026
                     /locus_tag="VFX87_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996142.1"
                     /translation="MTVEEALLAVVGAVALVLLFLGLVDALEGDPRAWLRGRRRRLSR
                     PERPRPGLAPAPRTPLARQASPTSPASARSAPKAAARAAAAAARTEPEAPAPAAPRAL
                     ATEEWRARVAALIRGRECGEARQLLEAALERGEVDPETAGFLVDVCSTAVARDLWRLR
                     RAVRRGGGDEAPLASSLEVTRVVLEAPVAQGLPREQRRRVTGRLWRGYTRLGLRRWRA
                     GTFEAAVESLFRALGVRGIDEQRRRLARDLLVRTLEDMAGESLELIPQLLGEGDRAIV
                     LGQAQRLLEHIRRTREEGVSAEDLAVAASRARQLLEHIEHTPVQ"
     gene            3023..4633
                     /locus_tag="VFX87_14260"
     CDS             3023..4633
                     /locus_tag="VFX87_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4996143.1"
                     /translation="MTELGPALTLGQMLDRAAARCPDRTAVVFKGERISYADLKRRAD
                     DFARGLLALGLRAGDHAVLWMPNRVEWNVANLGIAKIGAVTVTCNSRYKAFELEYVLR
                     QSDARALIMVDRFAAASVDYLEILRELGPGDRFPALRHVIVLGSDRPAGALGFEDVER
                     SGRTAGAALEAIRVRPEDPVEMLYTSGTTGEPKGCLLSHGNMYYKCRVYTELHAWTSE
                     DRCLVPVPYFHIFGSMGGIAANCLVASTQVLMDVFDPLEAMRLIEAERVTIFSGVPTM
                     FITVLGHPAFGTRDLRSLRTGSIGAAPVPVEIMRRILDRERGLGMDALVVYGLTEATG
                     GTHWTRPGDPLDRRVSTVGVRTAEILDRVVDPATGRELPAGEEGELCIKGPTLMIGYY
                     NKPEATAEKIRDGWLHTGDMAVKDADGYVRITGRLTDMIIVGGFNTYPAEIEDFFLRH
                     PKVLDVSIVGVPDAIMGEAVMAFVIPKEGASLEPEEIVAFARGQIANFKVPRYVEIVD
                     RFPLTGSGKVQKFKQKAYAVEKYALRGS"
     gene            complement(4584..4955)
                     /locus_tag="VFX87_14265"
     CDS             complement(4584..4955)
                     /locus_tag="VFX87_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4996144.1"
                     /translation="MTRESIPFLRDAIPVVHHHHERWDGSAYPLGLAGPAIPLGARIF
                     ALADAFDAMTPDRPYSKATSLEGGGIGSGTRRARISIRAWWPRSCACRSRSSRKRAPA
                     RSTRPARIPGARTSPPRRPSA"
     gene            complement(4952..>5541)
                     /locus_tag="VFX87_14270"
     CDS             complement(4952..>5541)
                     /locus_tag="VFX87_14270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4996145.1"
                     /translation="ARRLDTAYKELESTSAQLKEFSFKDEVTGLYNRRFFSIRLEEEV
                     SRYRRFNHPVSVVLLDLDGFKAVNDDLGHGAGDETLRAMAEILLKQSRGINVICRYGG
                     DEFAVLLVETSKSGARLYADRIRYVLSTWTFAHGRRVTASFGIASLPEDVAPAADELI
                     RAADEALYAAKRAGKNRVSVHEDVAAGRAPGRPGA"
BASE COUNT          774 a         1890 c         2079 g          798 t
ORIGIN      
        1 agggcgcgct cgtcaccggg gcgcagctca acgaggacgg caccgcgcgg atggacttcg
       61 atctgcccga cgccgacaac gatcggctcc gcgcgctcga gcccgcgatc aacgacgtca
      121 tccggcagga cctgccggtt cgctacgcgt acgtcaccga aagcgaagcc gtgtccgagc
      181 gcgggctcat ccggaaccga tcggtggcgc cgccgcccac cgcggacggg acgatccgcg
      241 tggtcgagat cgtgggcctc gatcgccagg cgtgcggggg aacccatctc gcgtccacgg
      301 cgggctccag gcagttgcgc gttctcaaga tcgacaacaa gggccggcac aaccggcgcg
      361 tgaagatcgg cctggtcgag acctcggagg ggtgaacgcc cggagggtgt cggtctcgag
      421 ctcgcctcag gcggggccga agccgctgac cgtctcgccg gcgagctcga tgggacggaa
      481 gcgcacgcgc aggccgctcg cgagcgcgtc gagcgggaga tccacgaggc gcccgagcag
      541 cgagacgccc tcgtcgagct tgaccaccgc gaccgcgtag ggcacttcgc cgccgcggcc
      601 ccgcggggcc acgcggatca cggtgtacga cacgatggcc ccggccccgg tgagcggcac
      661 cggctgccag tcgcgctgct ggcagcccgc gcagaactct ttcgggggaa tcgccagctc
      721 cccgcagcgg ccgcagcgga tgccggtgag tcggcccgcg cgcgcctctt cgaagaagct
      781 cttcaggttg atcacacgct cagtcacggc cgaacaggct cacgacgacg gtcgcggtgt
      841 tgccgccgag ggtatgggtg aggccggtgc gggcgccgtc cacctggcgc cggtccgcct
      901 cgccgcggag ctgggtgatg atctcggcga tctgagcggc cccggtggcg ccgatcgggt
      961 ggcccttggc cttgagcccg cccgacggat tcacggggag cttgccgcca aggctcgtcc
     1021 agcccttctc ggcggccagg ccgcccgtgc cgggctcgaa gaagccgagc ccctcggtgg
     1081 cgacgatctc ggcgatcgtg aagcagtcgt ggagctccac cacgtccacg tcggtagggg
     1141 tcttgccggc ctggcgatag gcgccctgcg cggcgcgctc ggtggcagtc acgcgggaca
     1201 ggtcgcgctt ctcgtgcacg aacatcgtgt cggtggccgc gtgcgtgccg agcacccaga
     1261 tcgggcggcg gtggcggcgg gccacctcct cggaggcgag caccagcgcg gcgcctccgt
     1321 cggtgaaggg gcagcagtcg aagagcttca gcggatccgc cacgtacgcg ctcttgagca
     1381 cggtctcgag cgtgatctcc ttctggaact gcgccttggg gttcagcacg ccgtgacggt
     1441 ggttcttgac cgcgacgtga gccatctgct cctcggtggt gccgtacttg tccatgtgcg
     1501 cccgcgcgat cagcgcgaac accccgggga aggtgaggcc ggcgggctgc tcgagggagg
     1561 cgtcggagca gtaggcgaag aactcggtgg cggactcggt cggcacgttc agcacgcgct
     1621 cggcgccgcc caccaggacc acgtccgaga cgccgctcgc gatctccatc accgcgtggc
     1681 ggaaggcgac gtggctggtc gcgcaggcgt tctcgaaccg ggtggtgggg agggtgggga
     1741 gccccaggag ggtggcggcc agcgggccca tgtggagctg gcgctcggtc tcgcctccgg
     1801 tcacgttgcc gaagtacatc gcctggaccg cattcggggc gatgccggcg tcggtgatgg
     1861 cgtcggcggc ggcggccgcg aacagctcgg cgctggtccg atcgtgcttg ccaaacttgg
     1921 tgacacccac tccgatcacg gcgacgcgac gcatggagtc tcctgagccc gctggaagta
     1981 ttggcgttcc ggtacccggc atcataccgc gccgcctcgc caaatgatcg tgaaagccag
     2041 gacggcgtga tacggtgggc cgcatgaccg tcgaagaagc cctgctggcg gtggtgggcg
     2101 cggtcgcgct cgtgctgctc ttcctcggtc tggtggacgc cctcgaaggg gatccgcgcg
     2161 cctggctccg cgggcggcgc cggcgcctct cgcggccgga gcggccgcgg cccggcctcg
     2221 cgccggcccc gcgcacgccg ctggcccgcc aggcgtcgcc gacctctccc gcttctgcgc
     2281 gttcggcgcc gaaggcggcg gcccgggcgg cggccgcggc agcccgtacc gagccggagg
     2341 ccccagcgcc ggctgcaccc cgcgccctcg cgaccgagga atggcgcgcc cgggtagccg
     2401 cgctgatccg cgggcgcgag tgcggcgagg cgcgccagct tctcgaagcc gcgctggagc
     2461 ggggagaggt ggatccggag acggcggggt tcctcgtgga cgtctgctcc acggcggtgg
     2521 cccgcgatct ctggcgcctg cgccgcgcgg tccgccgggg cggaggcgac gaggcgccgc
     2581 tcgcgagctc gctcgaggtc acccgcgtgg tcctcgaggc gcccgtggcg cagggtctcc
     2641 cgcgggagca gcggcggcgg gtgaccggcc gcctctggcg tggctacacg cggctcggcc
     2701 tgcggcggtg gcgcgccggg accttcgagg cggcggtcga gtccctgttc cgggcgctcg
     2761 gcgtgcgcgg gatcgacgag cagcggcggc gcctcgcgcg cgacctgctg gtgcgcacgc
     2821 tcgaggacat ggccggcgag agcctcgagc tcatcccgca gctcctgggc gagggagatc
     2881 gcgccatcgt gctcgggcag gcgcagcgac tcctcgagca catccgccgc acccgggaag
     2941 agggcgtgtc ggcggaggat ctggcggtgg ccgcctcgcg cgcgcgccag ctcctggagc
     3001 atatcgagca cacccccgtg caatgaccga gctcgggccc gctctgaccc tcggccagat
     3061 gctggaccgg gcggccgcgc ggtgtcccga ccggaccgcg gtcgtcttca agggcgagcg
     3121 catcagctac gcggacctca agcgccgcgc cgacgatttc gcccgcggcc tcctggctct
     3181 gggcctgcgg gcgggcgatc acgccgtgct ctggatgccg aatcgcgtgg agtggaacgt
     3241 cgcgaacctc ggcatcgcca agatcggggc ggtgaccgtc acgtgcaaca gccggtacaa
     3301 ggcgttcgag ctcgagtacg tgctacggca atcggacgcc agggccctga tcatggtgga
     3361 ccgcttcgcg gccgcctcgg tggactacct ggagatcctg cgtgagctcg gcccgggcga
     3421 ccgcttcccc gcgctgcgcc acgtgatcgt gctgggatcc gaccggcccg ccggcgcgct
     3481 cggattcgag gacgtcgagc ggtcggggcg cacggcgggc gccgctctcg aggcgatccg
     3541 cgtccggccc gaagatccgg tggagatgct gtacacctcc ggcaccaccg gcgagccgaa
     3601 ggggtgtctg ctctcgcacg gcaacatgta ttacaagtgc cgcgtctaca ccgagctcca
     3661 cgcctggacg tcggaggacc ggtgcctggt gccggtgccg tacttccaca tcttcggctc
     3721 gatgggcggc atcgcggcga actgcctggt ggcctccacc caggtcctca tggacgtgtt
     3781 cgacccgctc gaggccatgc ggctcatcga ggccgagcgg gtgacgatct tctcgggggt
     3841 gcccaccatg ttcatcacgg tgctgggcca ccccgcgttc ggtacccgcg atctccgctc
     3901 gctgcgaacc ggttccatcg gcgccgcgcc ggtgccggtg gagatcatgc ggcggatcct
     3961 cgaccgcgag cgtgggctcg gcatggacgc gctggtggtc tacgggctca ccgaggcgac
     4021 cgggggcacc cactggacgc ggccgggcga tccgctggac cggcgggtgt ccacggtggg
     4081 ggtgcgcacc gccgagatcc tggaccgcgt ggtggatccc gcgaccggcc gcgagctgcc
     4141 ggcgggggag gagggtgagc tgtgcatcaa ggggcccacc ctcatgatcg gctactacaa
     4201 caagcccgag gccaccgcgg agaagatccg cgacggatgg ctccacaccg gggacatggc
     4261 ggtgaaggac gcggacgggt acgtgcggat caccggacgg ctcaccgaca tgatcatcgt
     4321 cggcgggttc aacacctacc cggcggagat cgaggacttc ttcctgcgtc accccaaggt
     4381 gctggacgtt tccatcgtgg gagtgccgga cgcgatcatg ggggaggcgg tcatggcctt
     4441 cgtgatcccg aaggagggcg cctcgctcga gcccgaggag atcgtcgcct tcgcgcgtgg
     4501 acagatcgcc aacttcaagg tgccgaggta cgtggagatc gtggaccggt tcccgctgac
     4561 cggctccggc aaggtgcaga agttcaagca gaaggcctac gcggtggaga agtacgcgct
     4621 ccggggatcc tagcaggccg tgtcgaccga gcgggcgcgc gcttccgcga agacctcgag
     4681 cggcatgcgc aggaacgtgg ccaccacgcc cggatcgaga tgcgtgcccg ccgagtcccg
     4741 gatccgatcc cgccgccttc cagcgaggtg gccttcgagt agggccggtc gggggtcatg
     4801 gcgtcgaagg cgtcggcgag cgcgaagatg cgggcgccca gagggatggc cgggccggcg
     4861 agcccgagcg gatacgcgct tccgtcccag cgctcgtggt ggtggtgcac caccgggatc
     4921 gcgtcgcgca ggaacgggat gctctcgcgg gtcacgcccc cggccggccg ggggcccggc
     4981 cggccgcgac atcctcgtgt accgacacgc ggttcttgcc cgcgcgcttc gccgcgtaga
     5041 gcgcctcgtc ggccgcgcgg atcagctcgt cggcggcggg ggcgacgtcc tcgggcaggg
     5101 aggcgatgcc gaagctggcg gtgacccggc ggccgtgggc gaaggtccag gtggagagca
     5161 cgtagcggat gcggtcggcg tagaggcgcg cgccgctctt cgaggtctcg accaggagca
     5221 ccgcgaactc gtcgccgccg taccggcaga tgacgttgat gccgcgcgac tgcttgagca
     5281 ggatctcggc catcgcgcga agcgtctcgt cgccggcgcc gtggcccagg tcgtcgttca
     5341 ccgccttgaa gccgtcgagg tcgagcagca ccaccgagac ggggtggttg aagcgtcggt
     5401 agcgcgagac ttcttcctcg agccggatcg agaagaagcg gcggttgtag aggccggtga
     5461 cctcgtcctt gaacgagaac tccttgagct gggcgctggt ggactcgagc tccttgtagg
     5521 cggtgtcgag ccggcgcgcg t
//
LOCUS       DASUFA010000393         7214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_433749, whole genome shotgun sequence.
ACCESSION   DASUFA010000393 DASUFA010000000
VERSION     DASUFA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433749"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFX87_14275"
     CDS             complement(<1..230)
                     /locus_tag="VFX87_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX4996146.1"
                     /translation="MTWWLAALVLFGVLTGLFAIGLPVAFAFLGINVVGAWVWLGGDA
                     GLVQMIRNGVASIASFSLTPIPFFILMGEILF"
     gene            complement(227..700)
                     /locus_tag="VFX87_14280"
     CDS             complement(227..700)
                     /locus_tag="VFX87_14280"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX4996147.1"
                     /translation="MPARAYLGVLHAGAVAAALLLGALALLVTADVVARNVGWGTLPW
                     ILEVSEYCLPLATFLVAPWLLSRSEHVRVDVLLTMPPRRIARALERAADAAGVAVSAV
                     FVVYGIRAIVGSAQQGSLVMKSIVFPEWWLFAPVPVSFAFLAVEFLRRLLRAGSP"
     gene            complement(721..1680)
                     /gene="dctP"
                     /locus_tag="VFX87_14285"
     CDS             complement(721..1680)
                     /gene="dctP"
                     /locus_tag="VFX87_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX4996148.1"
                     /translation="MTRLIYCLIAVLLLAPAAGAQEVTLKVVSAFPENSLYVKRLETW
                     IARVNDEGKGVLRLNFIGGPKAVPTFEVGNAVRTGVVDVAMSTGAFYTSVFPESDALK
                     LTQMPIAEQRKNGAYDYISRVWSQRGNMHYLARIVEHQTFHVYLNKKIDKPDLTGLKI
                     RVTPVYRDFFTALGATVMQTAPGEVYTALERGVVDGYGWPIGGIFDLNWQEKTKYRVD
                     PGFYDAEVSLLVNQDAWKKLSQTQRDFLTRQALALEGQNDFWKTYARDETARQAQAGI
                     QVIRFEGEQAKQYVDRAYEAGWAGVLKASPEHGPKMRELFSRR"
     gene            1826..3151
                     /locus_tag="VFX87_14290"
     CDS             1826..3151
                     /locus_tag="VFX87_14290"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX4996149.1"
                     /translation="MLSREDNELLTRTGPGTAMGALIRQYWIPVVESKELEPGGRVKR
                     VMLLGERLIVFRGRSGRAGLIGEFCPHRSASFYYGRVEENGMRCAYHGWKFGLDGQCQ
                     EMPSEPPESSFASKVTHTAYPCLEKGGVVWAYMGPASPPPPLPELEFTLLPEAHTFIS
                     RRVQDCNWFQAMEGGIDSSHISFLHAPIDHRNSEVTQDMDRVSFGVGAAVSTGDRAPR
                     FEVADTDYGVVIGARRANPDGRWYWRITQFLLPFYTMPPTDLDERIVQSHIWVPMDDT
                     HSVNWMVTWHPDRKLTGEEIRLNIEGRGAHVCDYAPATSEPYGDVRTAANRDNDYFMD
                     WEIHRTRMYCGIPGFGVQDQAVQESQGDIVDRSQERLGSSDTAIIQVRRRMMTAARAL
                     RDHGVPAPGANPRSFLVRSTSVVLAPGESWIAGAMARMVVRAGDQLTLA"
     gene            3151..3927
                     /locus_tag="VFX87_14295"
     CDS             3151..3927
                     /locus_tag="VFX87_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4996150.1"
                     /translation="MPGRMAGRVALVTGGGGGIGEATGRLFAEEGAAVTLLDTDVAAV
                     ESSAAGILRAVPGAQVQALTGDVTRESDVRRIVDETAARWGALHTLVNVAGVRVYVPL
                     ADADAADWERIIGVNVLGTAHCCKAALPRLRAAGRGTIVNVSSVYGVMGRIGMGQYDT
                     TKAAVLGFTRSLAVEEAPHRIRVNAVCPGGTITPYHIRRAAARGVSEAEMRTQRAGDN
                     LLGRWAEPREVAYPILFLASDESSFVTGATLMVDAGRSIL"
     gene            3963..5063
                     /locus_tag="VFX87_14300"
     CDS             3963..5063
                     /locus_tag="VFX87_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX4996151.1"
                     /translation="MDALRGLAVAGMVLVNNPGTWSAVYAPLQHAAWHGWTPTDMVFP
                     FFLFVVGVSIPLALGSALERGADGLPLRVLRRAAVIFALGLLLHALPFFPLAELRIPG
                     VLQRIAVCYLLAALLVWATRGARGWRVQAVAAGVLLVGYWLLMTRVAAPGQVAGDLSP
                     AGNLSGYVDRLILGPRHIWQAARVYDPEGILSTLPATATTLLGVLAGHWLRAGQSGGL
                     PGARVAGGLAAAGVAATGLGWLWGLTFPVNKSLWTSSYALLMAGLAALALAACHWAIE
                     VRGRRGWAVPFAVLGVHALTLFFLSSLMAKLLFIVRAGAGGPRLQTWLFEHLFAPWAS
                     PMNASLAYALAYVLLWWALMWALDRSGLRLRV"
     gene            complement(5020..5667)
                     /locus_tag="VFX87_14305"
     CDS             complement(5020..5667)
                     /locus_tag="VFX87_14305"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX4996152.1"
                     /translation="MKLARAFYTRGDAVTVARRLLGSLLVARTRAGRRVSGIIVETEA
                     YQGPEDRASHAHGGRRTRRTETMYARGGTAYVYFVYGMYHQFNVVTNVADVPHAVLVR
                     ALEPVEGVELMRRRRGGVAPGELTSGPGKLCIALGIDRRLDRADLLGDRIWLEAGRPV
                     RPAAIAAGPRIGIDYAEDWVARPWRFWLRGNPFVSRAHPALVRRVAATRSGRAPT"
     gene            complement(5664..>7214)
                     /locus_tag="VFX87_14310"
     CDS             complement(5664..>7214)
                     /locus_tag="VFX87_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEX4996153.1"
                     /translation="TRHVRRLGPHRYDLWRPWYVPSLALGALALFALPMAQPITVKQV
                     GGRSKTQVAWIASRHQMALDDYVLNQAVVDPLWDLVREYLPVAITGGRPPYRLEPAEA
                     LLLAQRLLGIPEGDRPYPLLRPLRGTDGLGIRNVVLIQVEGLGATLLERDEPQGPVMP
                     FLRSLAAEGLYFPRVYQSFPSTDGAVFATLTSLHWTHALGGSGVRLSQSVMGTYFASL
                     PRLLTAPGAHHYAFSGFRHRTAEITSFQRNLGFHAAGFDALDARLGPAAAGPLGINDG
                     PLLLEAARVVTASPRPFTLYVMTGTSHSPWQVPPEAPMPLGNTPLGTFRYTDDSIRTF
                     VERLRAARPDFDQTLLVVIGDHTGPTFGTHPLERLRLPLILAGPPIARARARWAALPD
                     RTASEVDVLPTIASLLEGDHPYAGMGRSLFEPSVTAGIISGDTKETFYFKDGFALRYR
                     LHAGQAELLAVDGDAMPSADLSHEHPDVASRLTREFLALYETTDRLMRENRLFPIKPG
                     AARAPRAP"
BASE COUNT         1001 a         2475 c         2711 g         1027 t
ORIGIN      
        1 tggaagagga tctcgcccat gaggatgaag aacgggatcg gggtgaggga gaagctcgcg
       61 atggaggcga ccccgttgcg gatcatctgg acgaggcccg cgtccccgcc cagccagacc
      121 cacgcgccga ccacgttgat cccgaggaag gcgaaggcca ccggcaggcc gatcgcgaag
      181 aggcccgtga gcacgccgaa caggacgagg gccgcgagcc accaggtcat gggctccccg
      241 cgcgcaagag acgccgcagg aactcgacgg cgaggaaggc gaagctcacc ggaaccggcg
      301 cgaagagcca ccactccggg aagacgatgg acttcatcac gagcgagccc tgctgggcgg
      361 agccgacgat cgcccggatc ccgtagacga cgaacacggc cgacaccgcg acgccggccg
      421 cgtcggccgc gcgctcgagc gcccgggcga tccggcgcgg cggcatcgtg agcagcacgt
      481 ccacccgcac gtgctcgctt cgggagagca gccacggggc gaccaggaac gtggcgagcg
      541 gcaggcagta ctcggagacc tcgaggatcc agggcagcgt gccccagccg acgttgcgcg
      601 ccaccacgtc ggcggtgacg agcagcgcga gggcgcccag cagcagagcg gcggccaccg
      661 cgcccgcgtg gagcactccc aggtaggcgc gggccggcat cggagcgcgc gagagcccgg
      721 ctagcgccgg gagaacagct cacgcatctt cgggccgtgc tcggggctcg ccttgaggac
      781 gccggcccag ccggcctcgt aggcgcggtc tacgtactgc ttcgcctgct cgccctcgaa
      841 gcggatcacc tggatgccgg cctgggcctg ccgcgcggtc tcgtcccggg cgtaggtctt
      901 ccagaagtcg ttctggccct cgagggccag cgcctggcgc gtgaggaagt cgcgctgggt
      961 ttgggacagc ttcttccagg cgtcctggtt gaccaggagc gagacctccg cgtcgtagaa
     1021 tccgggatcc acgcggtact tcgtcttctc ctgccagttg agatcgaaga tgccaccgat
     1081 gggccagccg tagccgtcca ccacgccccg ctccagcgcg gtgtagacct cgccgggagc
     1141 ggtctgcatc acggtggcgc ccagcgcggt gaagaagtcg cggtacaccg gggtcacgcg
     1201 gatcttgagg ccggtgaggt ccggcttgtc gatcttcttg ttgagataga cgtggaaggt
     1261 ctggtgctcc acgatacgcg ccaggtagtg catgttgccc ctctggctcc acacgcggct
     1321 gatgtagtcg taggccccgt tcttccgctg ctcggcgatg ggcatctggg tgagcttgag
     1381 cgcgtccgat tccgggaaga cgctggtgta gaaggccccg gtggacatcg cgacatccac
     1441 cacgccggtg cgcaccgcgt tgcccacctc gaaggtcggg accgccttgg gcccgccgat
     1501 gaagttgagg cgcagaacgc ccttgccctc gtcgttcacc cgggcgatcc aggtctcgag
     1561 ccgcttgacg tagagcgagt tctccgggaa cgcgctcacc actttcaacg tgacttcctg
     1621 agcgcctgcc gccggtgcga gcagcaggac ggcgatgagg cagtagatga gtctcgtcat
     1681 ggacccctcc tggaggtggc gcccggagtc gcgtgggctg cggccggaga ttaggggagg
     1741 ccgcgtccat tgtcaattct cggcgaccgg ctggctggcg acccgccgga ccgcgtgcta
     1801 accttcggcg gaggagggcc ttccgatgct gagccgcgag gacaacgagc tcctgacccg
     1861 aaccggaccg ggcaccgcga tgggcgccct gatccggcaa tactggatcc cggtggtgga
     1921 atcgaaggag ctggagccgg gcgggcgggt gaagcgcgtg atgctgctcg gcgagcgcct
     1981 catcgtgttc cgcgggcgga gcggccgggc cggtctcatc ggcgagttct gcccgcaccg
     2041 ctccgcctcg ttctactacg ggcgcgtcga ggagaacggg atgcgctgcg cctatcacgg
     2101 ctggaagttc ggcctcgacg gccagtgcca ggagatgccg agcgagccgc ccgagtcgag
     2161 cttcgcgagc aaggtcacgc acaccgcgta cccgtgcctg gagaagggtg gggtggtctg
     2221 ggcctacatg gggccggcca gcccgccccc gccgctaccc gagctcgagt tcacgctgct
     2281 gcccgaggcg cacacgttca tctcgcgccg cgtgcaggac tgcaactggt tccaggccat
     2341 ggagggcggc atcgactcga gccacatctc gttcctccac gcgccgatcg accatcggaa
     2401 cagcgaggtc acccaggaca tggaccgggt gagcttcggg gtgggtgcgg cggtctcgac
     2461 cggggatcgg gcgccgcgct tcgaggtggc cgacaccgac tacggggtgg tgatcggcgc
     2521 gcgccgggcg aatccggacg gccgctggta ctggcgcatc acgcagttcc tgctgccctt
     2581 ctacacgatg ccgcccaccg acctggacga gcgcatcgtg cagtcgcaca tctgggtgcc
     2641 gatggacgac actcactcgg tgaactggat ggtgacctgg catcccgacc gcaagctgac
     2701 cggcgaggag atccggctca acatcgaggg gcggggcgcg cacgtctgcg actacgcgcc
     2761 ggccacctcc gagccctacg gcgacgtgcg caccgcggcc aaccgggaca acgactactt
     2821 catggactgg gagatccatc gcacccgcat gtactgcggc atcccgggct tcggggtgca
     2881 ggaccaggcc gtccaggaga gccagggcga catcgtggac cgcagccagg agcgcctcgg
     2941 ctcgagcgac accgcgatca tccaggtgcg ccggcggatg atgacggcgg cccgggcgct
     3001 ccgcgatcac ggcgtccccg cgcccggcgc caacccgcgc tccttcctgg tccggtccac
     3061 ctccgtcgtg ctggctccgg gcgagagctg gatcgcgggc gccatggcgc ggatggtggt
     3121 gagggcgggc gaccagctca cgctcgcctg atgccgggcc ggatggccgg ccgggtggcg
     3181 ctcgtcaccg gcggcggcgg aggcatcgga gaggccaccg gccggctgtt cgcggaggaa
     3241 ggcgccgcgg tcacgctgct ggacacggac gtggccgcgg tcgagtcgtc ggccgcgggg
     3301 attctgcgcg cggtgccggg ggcgcaggtc caggccctca ccggtgacgt cacccgagag
     3361 agtgacgtcc gcaggatagt cgacgagacg gcggcccgct ggggggcgct tcacacgctg
     3421 gtcaacgtcg cgggcgtgcg cgtctacgtg ccgctcgcgg acgcggacgc ggcggactgg
     3481 gagcggatca tcggggtcaa cgtgctcggc accgcccact gctgcaaggc ggcgctgccg
     3541 cggctgcgcg cggcggggcg cgggaccatc gtcaacgtct cgtcggtcta cggcgtgatg
     3601 gggcggatcg gcatgggcca gtacgacacc accaaggcgg cggtcctcgg cttcacgcgc
     3661 tctctggcgg tggaggaggc tccgcaccgc atccgggtca acgcggtgtg cccgggcggc
     3721 accatcacgc cctatcacat ccgccgcgcg gcggcccgcg gggtgtccga ggccgagatg
     3781 cgcacgcagc gcgcgggcga caacctgctc ggccgctggg cggagccgcg cgaggtcgcg
     3841 tatccgatcc tgttcctcgc ctccgacgag tcgtccttcg tcacgggcgc caccctgatg
     3901 gtggacgccg gccgctccat cttgtgacgc cgccgcccgg cgcggcggca cgcctgctct
     3961 ccgtggacgc gctccgcggg ctcgcggtgg ccgggatggt gctggtcaac aacccgggca
     4021 cgtggagcgc ggtgtacgcg ccgctccagc acgcggcgtg gcacgggtgg acgccgaccg
     4081 acatggtctt cccgttcttc ctcttcgtgg tgggcgtctc gatccccctg gccctgggct
     4141 cagcgctcga gcggggcgcg gacgggctgc ccctgcgtgt tctccggcgc gcggcggtga
     4201 tcttcgcgct cggcttgctg ctccacgcgt tgcccttctt cccgctcgcc gagctccgca
     4261 tcccgggcgt gctccagcgc atcgcggtct gctacctgct ggccgcgctg ctggtgtggg
     4321 ccacccgcgg cgcccgcggc tggcgcgtcc aggcggtcgc cgcgggcgtg ctgctggtgg
     4381 gctactggct cctgatgacc cgggtggccg cgcccggcca ggtggccggc gacctgagtc
     4441 ctgcgggtaa cctgtccggc tacgtggatc gtctcatcct cggcccgcgg cacatctggc
     4501 aggccgcgcg cgtctacgat ccggagggca tcctgagcac gctgcccgcc accgcgacca
     4561 cgctcctggg cgtgctggcc gggcactggc tgcgcgccgg gcaatcgggc ggcctgcccg
     4621 gagcgcgggt cgccggtggc ctcgcggcgg ccggggttgc cgcgaccggg ctcggctggc
     4681 tctggggcct cacgttcccg gtgaacaagt ccttgtggac cagctcgtat gcgctcctga
     4741 tggcggggct ggccgcgctc gcgctggccg cctgccactg ggcgatcgag gtgcgcggcc
     4801 gccgcgggtg ggccgtgccc ttcgcggtgc tgggcgtcca cgcgctcacg ctgttcttcc
     4861 tgtccagcct gatggcgaag ctcctgttca tcgtccgggc gggcgccggc gggccgcggc
     4921 tgcagacgtg gctcttcgag cacctcttcg cgccctgggc ctctccgatg aacgcgtcgc
     4981 tggcttacgc gctggcgtac gtgctgctgt ggtgggcgct catgtgggcg ctcgaccgga
     5041 gcgggttgcg gctacgcgtc tgacgagcgc cgggtgggcg cggctcacga acgggttgcc
     5101 gcgcagccag aaccgccacg gccgcgcgac ccagtcctcc gcgtagtcga tgccgatgcg
     5161 cgggccggcc gcgatggcgg cggggcggac ggggcggccc gcctcgagcc agatccggtc
     5221 gcccagcagg tccgcgcggt cgagacggcg gtcgatgccg agcgcgatgc agagcttgcc
     5281 cgggccgctg gtcagctcgc ccggcgccac cccgccgcgg cgccggcgca tcaactccac
     5341 gccctcgacc ggctcgagcg cccgcacgag caccgcgtgg ggcacgtccg cgacattggt
     5401 caccacgttg aactggtggt acatgccgta gacgaagtag acgtaggccg tcccgccgcg
     5461 cgcgtacatc gtctcggtcc ggcgggtgcg ccggccgccg tgggcgtggg aagcgcggtc
     5521 ctcggggccc tggtaggcct cggtctccac gatgatcccg gacacgcgcc gcccggcgcg
     5581 cgtgcgtgcc accaggagag agcccaggag ccgccgggcc accgtgaccg cgtcgccgcg
     5641 cgtgtagaac gcgcgcgcca gcttcacggc gcccgcggtg cgcgggccgc gcccggcttg
     5701 atcgggaaga ggcggttctc gcgcatgagg cggtcggtgg tctcgtacag cgcgaggaac
     5761 tcgcgggtga gccggctcgc cacgtccggg tgctcgtgcg agagatcggc ggagggcatc
     5821 gcgtcgccgt ccaccgcgag cagttcggcc tggcccgcgt gcagccggta gcgcagcgcg
     5881 aagccgtcct tgaagtagaa cgtctccttc gtgtcgccgc tgatgatccc ggcggtgacc
     5941 gagggctcga agaggctccg ccccatgccc gcgtaggggt ggtcaccctc gagcagcgag
     6001 gcgatcgtcg gcagcacgtc cacctcgctc gcggtccggt ccgggagcgc ggcccagcgg
     6061 gcgcgcgcgc gggcgatcgg cgggcccgcc aggatcagag gcaggcggag ccgctcgagc
     6121 gggtgggtgc cgaaggtggg gcccgtgtga tcgccgatga cgaccaggag ggtctggtcg
     6181 aagtcgggcc gcgcagcccg gagccgctcc acgaacgtac ggatgctgtc gtcggtgtag
     6241 cggaaggtgc cgaggggcgt attgccgagc ggcatgggcg cttctggggg cacctgccac
     6301 ggcgagtggc tcgtcccggt catcacgtag agggtgaagg gacgcgggct cgcggtcacc
     6361 actcgcgccg cctccaggag gagcgggccg tcgttgatgc cgagcgggcc cgccgcggcc
     6421 ggcccgaggc gcgcgtcgag cgcgtcgaag ccggcggcgt ggaagcccag gttgcgctgg
     6481 aagctcgtga tctcggcggt gcggtggcgg aagcccgaga aggcgtagtg gtgggctccc
     6541 ggcgcggtca gcaggcgcgg gagcgaggcg aagtaggtgc ccatgaccga ctgggagagg
     6601 cgcacgccgc tgccacccag ggcgtgggtc cagtgaagac tggtgagcgt cgcgaagacc
     6661 gcgccgtccg tcgacgggaa gctctggtac acccgcggga agtagaggcc ctccgcggcg
     6721 agcgagcgga gaaacggcat gaccgggccc tgcggctcgt cgcgttcgag cagggtggcg
     6781 ccgagcccct cgacctggat cagtacgacg ttccggatcc cgaggccgtc ggtcccgcgg
     6841 agggggcgca ggaggggata cggccgatcg ccctcgggga tgccgagcag ccgctgggcg
     6901 agcagcagcg cttccgcggg ttcgagccgg tacgggggcc gcccgcccgt gatggccacc
     6961 ggcaggtact cgcggaccag gtcccagagc gggtcgacca ccgcctgatt gagcacgtag
     7021 tcgtccagcg ccatctggtg ccgactcgcg atccacgcga cctgggtctt ggaccggccg
     7081 cccacctgct tgaccgtgat cggctgcgcc atcggaagcg cgaatagcgc gagcgcgccg
     7141 agcgcgaggg aggggacgta ccaggggcgc cacagatcgt agcggtgagg gcccagccgc
     7201 cggacgtgac gggt
//
LOCUS       DASUFA010000394         7100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1599692, whole genome shotgun sequence.
ACCESSION   DASUFA010000394 DASUFA010000000
VERSION     DASUFA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599692"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFX87_14315"
     CDS             <1..439
                     /locus_tag="VFX87_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4996154.1"
                     /translation="QLGAIRIAVIPGTTTEQMVKNAIASMGATAADARLVVVNDHADG
                     LAAVVERRAEVYATDRAILMGLVASASDPRQFVLLDRYLSYEPYALMLRRGDPEFRLA
                     VNRTLARLYRSGQVLDIYRRWFGQWRDPSPLTLGMYAIEGLPE"
     gene            590..1453
                     /locus_tag="VFX87_14320"
     CDS             590..1453
                     /locus_tag="VFX87_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX4996155.1"
                     /translation="MRATTRLRQMLKAPGIIVAPGAYDGFSARLIEAAGFQAVYMTGA
                     GTAASHLGQPDLGLATLTEMANHAGHLASCVSLPVVADADTGYGNVLNVVRTVREYEK
                     AGVAGLHIEDQVAPKKCGHIAGKQVIPTEEFCDKVRAASEYRTDPDFIIIARTDARAV
                     TSLEDAIDRGNRYAQAGADLIFFEAPQTEDEIRRVAREIKAPLLANMVIGGKTPAMKV
                     AELERIGFKMVIFPAVCMAAAVLAMENSLAFLKEHGTDWQPGPVLGPMDIFRRVGFDW
                     WHGIEEKFAGR"
     gene            1453..2856
                     /gene="leuC"
                     /locus_tag="VFX87_14325"
     CDS             1453..2856
                     /gene="leuC"
                     /locus_tag="VFX87_14325"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX4996156.1"
                     /translation="MTSQRLTMFEKIWSRHVVAEGPGGQALLYVDRHLLHEGATHALA
                     RLERKGRPVRRPEACLATADHYVPTEPGAGEIADPEIRAMVADLGRHSRRLGITFFGR
                     GEPRQGIVHVVGPEQGATQPGITLMCGDSHTATHGAFGALACGIGSTEVEHVLATQCL
                     WQRRPKVMRIAVTGTRPFGVMAKDVILAIIARIGAGGGVGHAIEYAGPVICAMSMDER
                     MTVCNMSIEAGARAGMVAPDETTFAYLEGRPLAPRGEMWPRAVAFWKTVPSDADAVYD
                     RDITLDAAELAPTVTWGTSPQDALPITGHVPDPSAVGDPVRRAGMERALGYMALTPGA
                     ALAGTAVSRVFIGSCTKSRLEDLRAAAAVAKGRRTAVPAWVVPGSAQIKRAAEAEGLD
                     RVFTEAGFEWREPGCSMCVGMNGDTAAAGERVASTSNRNFEGRQGQGVRTHLMSPAMA
                     AAAAVTGRLTDVRTLGR"
     gene            2864..3472
                     /gene="leuD"
                     /locus_tag="VFX87_14330"
     CDS             2864..3472
                     /gene="leuD"
                     /locus_tag="VFX87_14330"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX4996157.1"
                     /translation="MEPFTRLTAVAVPMDLPNIDTDRVIPARFLRKPREAGFGPLLFH
                     DVRFDAAGAPQPDFVLNHPAYRDAKILVTAENFGCGSSREMAVWALMDYGIRVVIGPS
                     FGDIFIENCFKNGALAVVLPSDVVAGLRRALARRPGATLTVDLESQSITAPAGEAIRF
                     EIDAFRKHGLLTGQDEVEMTLARLPAIEAFEARRRVEMPWLG"
     gene            3465..3884
                     /locus_tag="VFX87_14335"
     CDS             3465..3884
                     /locus_tag="VFX87_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996158.1"
                     /translation="MAERTGVLVAFFAATWLTGLSLATAFAAAAYGFGRSVEPERTDL
                     ISLAIFGVMGLASQAGLLLAPWATTRGLTSRIIAALLMGPAGVSLSIFAYEGFTRYAA
                     GSPTWVVTWGVYLWGVLVYAVAYVALVRGGLGRTRET"
     gene            3946..4608
                     /locus_tag="VFX87_14340"
     CDS             3946..4608
                     /locus_tag="VFX87_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996159.1"
                     /translation="MAIPIESSPPPGWEREGIDGLLGLFPPGRLSEIVGPRSSGGSSL
                     LLALIARITTSGSRGGSQGSGQVAFVDGMDALDPASAAAAGADLSALLWVKCGGRLRV
                     ALSAADLLVRCPGFALVVVDLDELGPMPASAHLRLQRAVEGGDTILIIRSARHREGSP
                     ASLVLATRRLAPRWMGLRPITRLAGLRSEVSIFRSRSSQTLSPLGRGQGEGKAWTLDW
                     TL"
     gene            4632..5888
                     /locus_tag="VFX87_14345"
     CDS             4632..5888
                     /locus_tag="VFX87_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996160.1"
                     /translation="MNEPARPALLRVALAHSPRVEDAGADRVYLDASGLAGLFGDEPR
                     LAARLRDAAAAAGVESRVGIAESRIAALAAARLGPGVMIVEPGGDAAYLAPASLSLLD
                     LPEETATRLSRWGLRTLGELAALPTAGLFERLGGDGVRHQRLARGDDPRPLVSWRPVP
                     LFEESAACEWGLETLEPVVERLRELAARVCTRLGAAGLAADGFEWACRLGDGRLHEGA
                     LIPAVPITDAESVGGLLRLALEARPPRGVVEALTFRARPVRVAPAQESLTDRSRPAPR
                     LFTATLNRLVALVGADAIGAPALLDTHRPDAIALATYQPYPSPLRGEGRGAEARGEAP
                     ALALRRLRPPAPASVTLASGRPVAVRSGRLTGRIVAGVGPWRVSGEWWSPRPWLQDEW
                     DVELADGTLCRLAHDGSAWRLEGIYD"
     gene            5968..>7100
                     /locus_tag="VFX87_14350"
     CDS             5968..>7100
                     /locus_tag="VFX87_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX4996161.1"
                     /translation="MYVELHARSAFSFLEAAALPEALAERAARLEQPAIALLDADGVY
                     GAPRLYRACNRLGLSALVGAEITLADGSRLPLLVEDREGYQNLCRLLTRVKMRAPKGE
                     GAATFEELAEFAGGLVCLTGGGRGPVAAALARAGAPAGRECLDRLVGIYGRYDVFAEL
                     QRHLSRPEEARNEWLRAEAARLGLSPLATNEPRLIARGDRPLLDVLTCIREHSTLEAA
                     GRLLARNSEHFMKSGRVMERLFADSPEAVANTGELALRLGFTLKDLGYRFPDYPLPRG
                     ETPLGFLRALAHAGARDRYGSGPLAEKARRQIEHELALIGRLDLAGYFLIVWDLVQYC
                     RAHDVLVQGRGSAANSAVCYALGLTAVDPVGMELLFERFLSEER"
BASE COUNT          879 a         2591 c         2594 g         1036 t
ORIGIN      
        1 ccagctcggc gcgatccgga tcgcggtgat cccggggacc accaccgagc agatggtcaa
       61 gaacgcgatc gcgagcatgg gcgccaccgc cgccgacgcc aggctggtcg tggtgaacga
      121 tcacgccgat ggcctggccg cggtggtgga gcggcgcgcc gaggtctatg ccaccgatcg
      181 cgcgatcctc atggggttgg tggcgagcgc gagcgacccg aggcagttcg tgctgctcga
      241 ccgctatctc tcgtacgagc cgtacgcgct catgctccgg cgcggcgatc ccgagttccg
      301 gctcgcggtc aaccgcacgc tggcccggct ctaccgctcc ggccaggtgc tcgacatcta
      361 ccgtcgctgg ttcggccagt ggcgcgaccc gagcccgctc acgctcggca tgtacgcgat
      421 cgaggggctg cccgagtagc gtcggcccgt ccttgtcgcg ccccgccgcc cgcgtggatg
      481 tcgggtcatt tgacaatcgc gaggcgatcg gccgacaatc gtagttgtcg ccaagcctcc
      541 gatagcctgc agtccccgtc gggccgaacg cccgggaaag gtctctgtca tgcgagcgac
      601 gacgcgtctc cgccagatgc tcaaggcccc cgggatcatc gtcgcgccgg gcgcctacga
      661 cggcttctcg gcccgcctga tcgaggcggc tgggttccag gcggtgtaca tgaccggcgc
      721 gggcaccgcc gcgtcgcacc tcggccagcc ggacctgggc ctggccacgc tcaccgagat
      781 ggccaatcac gcggggcatc tggcctcgtg cgtctcgctg ccggtggtcg cggatgccga
      841 caccggctac ggcaacgtgc tcaacgtggt gcgcacggtg cgggagtacg agaaggcggg
      901 cgtggccggc ctccacatcg aggaccaggt ggcgcccaag aagtgcgggc acatcgcggg
      961 caagcaggtg atcccgaccg aggagttctg cgacaaggtc cgcgccgcct ccgagtaccg
     1021 caccgatccc gacttcatca tcatcgcccg cactgacgcc cgcgcggtca cctcgctcga
     1081 ggacgcgatc gaccgcggca accgctacgc gcaggcgggc gccgacctga tcttcttcga
     1141 ggcgccgcag accgaggacg agatccgccg ggtggcgcgg gagatcaagg cgccgctgct
     1201 cgcgaacatg gtcatcggcg gcaagacgcc cgcgatgaag gtcgccgagc tcgagcggat
     1261 cggcttcaag atggtgatct ttccggcggt gtgcatggcc gccgcggtgc tcgcgatgga
     1321 gaactcgctc gcgttcctga aggagcacgg caccgactgg cagcccggcc cggtgctggg
     1381 cccgatggac atcttccggc gcgtgggctt cgactggtgg cacggcatcg aggagaagtt
     1441 cgcggggcgg taatgacgtc gcaacgcctg accatgttcg agaagatctg gagccgccac
     1501 gtggtggcgg aggggcccgg tggccaggcc ctgctctatg tcgatcgtca cctgctgcac
     1561 gagggcgcca cgcacgcgct ggcgcgcctc gagcgcaagg gccgaccggt gcgccgtccc
     1621 gaggcttgcc tcgccaccgc cgaccactac gtgcccaccg agccgggcgc gggcgagatc
     1681 gccgacccgg agatccgcgc catggtggcg gatctcggcc gccacagccg gcgcctcggc
     1741 attaccttct tcggtcgcgg ggagccgcgt caggggatcg tgcacgtcgt cggccccgag
     1801 cagggcgcga cccagcccgg catcacgctc atgtgcggcg actcgcacac cgcgacccac
     1861 ggggccttcg gcgcgctcgc ctgcgggatc ggctccaccg aggtggagca cgtgctggcc
     1921 acgcagtgtc tctggcagcg gcggccgaag gtgatgcgga ttgcggtgac cggcacgcgc
     1981 ccgttcgggg tcatggccaa ggacgtgatc ctcgccatca tcgcccggat cggggcgggg
     2041 ggcggggtcg gccacgcgat cgagtacgcg gggccggtca tctgcgccat gtccatggac
     2101 gagcgcatga cggtctgcaa catgtccatc gaggcgggcg cccgcgcggg catggtggcg
     2161 cccgacgaga cgaccttcgc ctatctcgag ggccgccccc tcgcgccccg gggcgagatg
     2221 tggccgcgcg cggtcgcctt ctggaagacg gtgccctccg acgcggacgc ggtctacgac
     2281 cgggacatca cgctcgacgc ggcggagctc gcgcccaccg tgacgtgggg cacgagcccg
     2341 caggatgcgc tgccgatcac cgggcacgtg cccgacccga gcgcggtcgg cgatcccgtg
     2401 cgccgggccg gcatggagcg cgcgctcggc tacatggcgc tcacgcccgg cgcggcgctg
     2461 gcgggcacgg cggtgagtcg cgtgttcatc ggctcctgca ccaagagtcg gctcgaggat
     2521 ctgcgcgccg cggcggcggt ggccaaggga cgccgcacgg cggtgcccgc gtgggtggtg
     2581 ccgggctccg cgcagatcaa gcgcgcggcg gaggcggagg gactcgaccg cgtcttcacg
     2641 gaggcgggct tcgagtggcg cgagcccggc tgctcgatgt gcgtcggcat gaacggcgac
     2701 acggcggcgg cgggcgagcg cgtggccagc acctccaacc gcaacttcga gggccggcag
     2761 ggccagggcg tgcgcacgca tctcatgagc ccggccatgg ccgcggccgc tgcggtcacc
     2821 ggccgcctca ccgacgtccg cacgctcggt cgctgacccc gccatggagc ccttcacccg
     2881 cctcaccgcg gtggccgtgc cgatggatct gccgaacatc gacaccgacc gcgtgatccc
     2941 cgcgcgcttc ctccgcaagc cgcgcgaggc gggcttcggg ccgctcctct tccacgacgt
     3001 gcgcttcgac gccgccggcg ccccgcagcc cgacttcgtg ctgaaccatc ccgcgtaccg
     3061 ggacgcgaag atcctggtca ccgccgagaa cttcggctgt ggctcctcgc gcgagatggc
     3121 ggtgtgggcc ctcatggact acggcatccg cgtggtgatc gggccgagct tcggcgacat
     3181 cttcatcgag aactgcttca agaacggcgc gctcgcggtg gtgctgccgt cggacgtcgt
     3241 ggccggcctg cgccgcgcgc tcgcccggcg cccgggcgcg accctgacgg tggatctcga
     3301 gagccagagc atcaccgcgc cggccgggga agcgatccgc ttcgagatcg acgccttccg
     3361 caagcacggc ctgctcaccg ggcaggacga ggtcgagatg acgctcgccc ggctgcccgc
     3421 catcgaggcg ttcgaggccc gccgccgcgt cgagatgccc tggcttggct gagcgcaccg
     3481 gcgtcctcgt cgccttcttc gcggccacct ggctcaccgg gctgtcgctc gcgaccgcct
     3541 tcgccgccgc ggcgtacggc ttcggccgca gcgtggagcc ggagcgcacc gacctgatca
     3601 gcttggcgat cttcggggtg atggggctgg cctcgcaggc gggactgctg ctggcgccgt
     3661 gggcgaccac ccgcgggctc acgtcgcgca tcatcgccgc gctgctcatg gggcccgccg
     3721 gcgtgtcgct gagcatcttc gcgtacgaag gcttcacgcg gtacgcggcg ggctcgccga
     3781 cctgggtggt gacgtggggg gtctatctct ggggcgtgct cgtctacgcg gtggcgtacg
     3841 tggcgctggt ccgcgggggg ctcggccgga cgcgggagac gtagcctttt tttgtggttg
     3901 catatactaa acaggtgatg tgtaatgcgg ccctggtcac accgcatggc tatcccgatc
     3961 gagtcctcac ctccgccagg gtgggagcgc gaggggatcg acggcctgct cggtctgttc
     4021 ccgccgggcc ggctcagcga gattgtcggt ccccgatcct ccggaggtag cagcctgctc
     4081 ctggccctca tcgcccggat cacgacttcg ggcagccggg gcggtagtca gggtagcggc
     4141 caggtggcct tcgtggatgg gatggacgcg ctcgacccgg cttccgccgc ggcggcgggc
     4201 gccgatctct ccgcgctgct gtgggtgaag tgcggcggcc ggctccgggt ggcgctcagc
     4261 gccgccgatc tcctcgtccg ctgcccggga ttcgccctgg tcgtggtgga tctggacgag
     4321 ctcgggccca tgcccgcctc ggcccatctg cggctgcagc gcgcggtgga gggcggcgac
     4381 accatcctga tcatccggag cgcccgccat cgcgagggca gccccgcgtc gctggtgctg
     4441 gccacgcggc ggctggcgcc acgctggatg gggctgcgcc cgatcacccg cctcgccggc
     4501 ctccgctccg aggtctccat cttccgctcc cggtcttccc agaccctctc ccctctgggg
     4561 agagggcagg gtgaggggaa ggcctggacg ctcgactgga cgctatgact cgcatcgcct
     4621 gcctgctcgc cgtgaacgaa ccggcgcggc ccgcgctgct gcgcgtggcg ctcgcgcatt
     4681 ccccccgcgt ggaggacgcc ggcgccgacc gggtctatct cgacgcgtcc gggctcgcag
     4741 ggctcttcgg cgacgagcca cgcctggccg cgcggctccg cgacgccgcc gcggcggcgg
     4801 gagtggagag ccgtgtgggc atcgcggaga gccgcatcgc cgcgctcgcc gccgcgcgcc
     4861 tgggcccggg ggtgatgatc gtcgagcccg gtggcgatgc cgcgtatctc gccccggcgt
     4921 cgctctcgct gctcgacctg cccgaggaga cggccacgcg cctgtcgcga tgggggctcc
     4981 gcacgctcgg cgagctggcg gcgctgccca ccgcggggct gttcgagcga ctcggcggcg
     5041 acggcgtccg gcaccagcgc ttggcccgcg gggatgatcc gcgcccgctc gtctcgtggc
     5101 ggccggtgcc gctcttcgag gagtcggctg cgtgcgagtg ggggctcgag accctcgagc
     5161 cggtggtgga gcgcctgcgc gagctggccg cccgcgtctg cacgcgcctc ggcgcggccg
     5221 ggctcgcggc cgacggtttc gagtgggctt gtcggctcgg cgacggtcgc ctccacgagg
     5281 gcgcgctcat ccccgcggtg cccatcaccg atgccgagtc ggtcggaggg ctgctccgcc
     5341 tggcgctcga agcgcgcccg ccgcgcggcg tggtggaggc cctcacgttt cgcgcgcggc
     5401 cggtgcgggt cgcgccggcg caggagtcgc tcaccgatcg ctcgcgcccg gcgccgcgcc
     5461 tgttcaccgc caccttgaac cggctggtgg cgctggtggg cgccgacgcc atcggcgccc
     5521 ccgcgctgct cgacacccac cgccccgacg ccatcgccct cgccacctac cagccttatc
     5581 cctctcccct caggggagag ggcaggggtg cggaggcgag gggtgaggcg cccgccctcg
     5641 ccctccgccg cctccgcccc cccgcgcccg cgtcggtgac gctcgcctcc ggccggccgg
     5701 tggcggtgcg ctcgggccgc ctcacgggac gcatcgtcgc cggggtgggg ccgtggcgcg
     5761 tctcgggcga atggtggagc ccacgcccgt ggctgcagga cgagtgggac gtggagctgg
     5821 ccgacggcac gctctgccgc ctggcgcatg atggctccgc ctggcgcctc gaaggcatct
     5881 acgactaacg tccctcacct ccggccccct cacctccggc cccctcacct ccggccctct
     5941 cccctgaggg gagacggaaa acgaaatatg tacgtcgagc tgcatgcccg gagtgccttc
     6001 agctttctcg aggcggcggc gctgcccgaa gcgctggccg agcgcgcggc gcggctcgag
     6061 cagcccgcca tcgcgctgct ggacgccgac ggtgtctacg gcgcgccccg gctctatcgc
     6121 gcctgcaacc ggctcggcct ctcggcgcta gtgggcgccg agatcacgct ggccgacggc
     6181 agccggctgc cgctgctggt ggaagatcgc gagggctacc agaacctctg ccgcctgctg
     6241 acgcgcgtga agatgcgcgc gccgaagggg gaaggcgccg ccacgttcga ggagctggcc
     6301 gagttcgcgg gcggcctcgt ctgcctgacc ggcggcggcc gcggcccggt ggcggccgcg
     6361 ctggcgcgcg cgggtgctcc cgccgggcgc gagtgcctcg atcgcctcgt cggcatctac
     6421 ggccggtacg acgtcttcgc cgagctgcag cgccacctga gccgcccgga ggaggcgcgc
     6481 aacgagtggc tgcgcgcgga ggccgcgcgg ctcgggctgt ccccgctcgc caccaacgag
     6541 ccccggctga tcgcgcgcgg ggaccgcccg ctgctcgacg tgctcacgtg catccgcgag
     6601 cacagcacgc tggaggccgc gggccggctg ctcgcgcgca acagcgagca cttcatgaag
     6661 agcggccggg tgatggagcg gctcttcgcg gactcccccg aggcggtggc caataccggc
     6721 gagctggcgc tgcgtctcgg cttcaccctg aaggacctgg gctaccgctt ccccgactac
     6781 ccgctgcccc ggggcgagac gccgctgggc ttcctgcgcg cgctcgccca cgcgggagcc
     6841 cgcgaccggt acggctcagg gccgctcgcc gagaaggcgc gtcgccagat cgagcacgag
     6901 ctggccctga tcggccggct cgacctggcg ggctactttc tcatcgtctg ggacctggtc
     6961 cagtactgcc gcgcccacga cgtgctggtg caggggcgcg gctccgcggc caacagcgcg
     7021 gtgtgctacg cgctcggcct caccgcggtg gacccggtgg gcatggagct gctcttcgag
     7081 cgcttcctct ccgaggagcg
//
LOCUS       DASUFA010000395         6123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1145322, whole genome shotgun sequence.
ACCESSION   DASUFA010000395 DASUFA010000000
VERSION     DASUFA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145322"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2660
                     /locus_tag="VFX87_14355"
     CDS             <1..2660
                     /locus_tag="VFX87_14355"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX4996162.1"
                     /translation="ADGDRSVLTVGRIDTVGLDYTWPATATIERVHMKKSWVHIERRA
                     DGTLPITALFTPARPGPGPAPGPAAPAPPSSPETGKVSITVRETVFEDGSATIVDAAV
                     SPTARIDVAGVRLASRDFTWPARAPIPVQLDMPTPGAGTLTAKGQLDLTARALEAQVT
                     PSGVDVSPARPYLPVRGRIAGKASGDLQVKAMLEPLTITARGTATLADLAVADGDRPV
                     ATAARLETTGIDYTWPATVVVEAARLQKPWAQIERAADGSFPITALLTPAPPSATGGA
                     RREPAGEGTPPAGPAAPKVDVRVRRVAVEDGVIAIVDGAVSPPGRFQLQGASLQIENI
                     AWPAREASQVKLRTETASGGHLEAQGQLRVDTQAVDMQIAAKQLDLATMQAFLPSRGT
                     IEGKMDADLRVRGTLAPLAVAATGRLAFDDPTFGDGQRMLAYIKRVDVARLDADWPRR
                     VAVERVAIDKPWVLLERETDGTVPLLGLLMAGPSAPKTAPVPPPRDRAPTGAAAPPPV
                     VTVGALAVEEGFVRFVDRTTTPAFVEEASRIALTGRGLGTAPDSRGQVAMNGRLTGGA
                     PFEMNGTLGAIGGPLNLEVDGKLTDFPLTRVNPYSNKLIGWVARRGAFGATVHYRVVD
                     NRIEATNDLLIGQPEYAPSRSGDEVRERVGVPFGMLVSLLKNVRGEIRLSVPVSGDLA
                     SRQFDLSEAFWAAMRETAISVMALPVSWVGKIFYTEDARIETVQIWPVYFEAGSTRIV
                     RDFEQHAKRLATFLREAPGVTLALKPVHTVDDIVALKREAVRQRIDAGAREPGQTAEA
                     VAARLFAERFPGQAPPPGLDALVTELVKAEPNPDAAARNLAEQRMDITRARLEASKGT
                     ANAARLRITEGVVPVEASGQGRIEFEIVP"
     gene            complement(2716..3312)
                     /locus_tag="VFX87_14360"
     CDS             complement(2716..3312)
                     /locus_tag="VFX87_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4996163.1"
                     /translation="MAKTESDVRAVGSAQTPRGVNHVVLNVRDIEVSHRFWTEVVGFR
                     CVAELKPQPGKVRPKMRFYSGVADNGDLSHHDLALAEVPRKEGAPAAEGSPETWSLMG
                     PKVGLNHVAIAWPDRESWLKQLEFLQSRGVTFHRRINHGMTHSVYISDPDGHGIEILY
                     ELPREVWGRDIDAAQNYAETLPTEGPAALEDRTDNPKF"
     gene            complement(3397..4551)
                     /locus_tag="VFX87_14365"
     CDS             complement(3397..4551)
                     /locus_tag="VFX87_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996164.1"
                     /translation="MTLSPLETARKLAPLIRSSAPETDAQRELPRPLFEAMADAGLFR
                     LGLPRTLGGFEMDLPSYIQVIEEIGRADASTAWVTNQVSIFATYAARMETAAARAIWI
                     DTPRSVVANTPQANAQALVVPGGYRVTGRQGFSTGCRHASWLAAHTTVTERGRPRLDD
                     GQPEMRYCFVPRAEAQLLDTWQVRGMRGTGTHHFAVDDVFVPEERTVKSVTAPLVEQG
                     PLYRLPRTLVFASGDAAVALGTARSCLAAFMDLATTKTPRAMDALLRDQPMIQTEVGR
                     AEARLRSGRAFLREAVAEIWDAVVAHGAVTLEQRAVLRLATTDGIRTAVSVVDMVYNM
                     AGATAAYESSPIQRHFQDVHVMSQHLQARQAHYELVGRHWLGLKIDESRL"
     gene            complement(4548..4868)
                     /locus_tag="VFX87_14370"
     CDS             complement(4548..4868)
                     /locus_tag="VFX87_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX4996165.1"
                     /translation="MFFELRQYHIRPGQQEKWVKCMEEEIIPFQVKMGMVILGSFVGE
                     EDQSVYVWIRRFESEAERKRLYDAVYQSDYWKNEISARVGTMIDREQIKVTRLTATPH
                     SPIQ"
     gene            4966..6030
                     /locus_tag="VFX87_14375"
     CDS             4966..6030
                     /locus_tag="VFX87_14375"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX4996166.1"
                     /translation="MAPLVCGGCCDVRAVTAVTAVPLAAARRPRRAPDGAHRAAAGGR
                     REAAAGRPVATPGVQGYSPPPPPVSPIKGLIDFHTHAAPDIFGRALDDDELATLAATR
                     QMEAVVFKNHVTHTADRAFLVRKHVPGIKVFGGITLNQAVGGLNPQAVEWMWRMQGGF
                     GRVVWFPTFDADNHVKHAGTAPAGIRVVDERGQVLPAARAVLKICAAQRLVVQTGHAS
                     PDEALALIAAAREAGCERIVVTHAQFDVVGMSLAQMKKAAALGAKLELCALLMLAGPT
                     SPLEFMHHTARVEVSDTAARIKAVGAQHFVLGTDLGQAGNPTPADGLQMLVAGLMKEG
                     ITRDQIQTMGREIPGALLMG"
BASE COUNT          855 a         2253 c         2181 g          834 t
ORIGIN      
        1 tcgccgacgg cgaccggtcc gtgctcaccg tcggccgcat cgacacggtc ggcctcgact
       61 acacctggcc ggccaccgcc accatcgagc gcgtgcacat gaagaaatcg tgggtgcaca
      121 tcgagcggcg ggccgacggg accctgccga tcaccgcgct gttcaccccg gcgcgcccgg
      181 ggccgggccc ggccccgggc ccggcggccc ccgcgccccc gtcgagcccg gagaccggca
      241 aggtcagcat cacggtgcgc gagaccgtct tcgaggacgg cagcgcgacg atcgtggacg
      301 cggcggtgag cccgaccgcg cgcatcgacg tggccggcgt gcggctcgcc tcgcgcgact
      361 tcacctggcc cgcgcgcgcc ccgatcccgg tgcagctcga catgcccacc ccgggggcgg
      421 gaacgctcac cgcgaagggc cagctcgatc tcaccgcgcg agccctcgaa gcgcaggtca
      481 cgcccagcgg cgtggacgtc tcaccggcgc ggccctacct gccggtgcgc gggcggatcg
      541 cgggcaaggc gagcggcgat ctccaggtca aggcgatgct cgagcccctc acgatcaccg
      601 cgcgcggcac cgccaccctg gccgatctgg ccgtggccga cggcgaccgg ccggtggcca
      661 ccgctgcgcg gctcgagacc accgggatcg actacacgtg gccggcaacc gtggtggtcg
      721 aggccgcgcg gctgcagaag ccgtgggccc agatcgagcg cgccgccgac gggagcttcc
      781 cgatcaccgc gctgctcacg ccggcgccgc cttccgccac gggaggcgcg cggcgcgagc
      841 cggccggcga gggcaccccg ccggccggcc cggccgcgcc gaaggtggac gtccgcgtgc
      901 gccgcgtcgc ggtcgaggac ggcgtgatcg cgatcgtcga tggagcggtg agcccgcccg
      961 ggcgcttcca gctccagggc gcgagtctcc agatcgagaa catcgcctgg cccgcgcgcg
     1021 aggcgtccca ggtgaagctg cgcacggaaa ccgcgagcgg cggccacctg gaggcgcagg
     1081 ggcagcttcg cgtggacacc caggccgtgg acatgcagat cgcggccaag cagctcgacc
     1141 tggcgacgat gcaggccttc ctgccctcgc gcggcaccat cgagggcaag atggacgccg
     1201 acctgcgcgt gcgaggcacg ctggcgcccc tggccgtcgc ggccaccggc cgcctcgcct
     1261 tcgacgatcc caccttcggc gacgggcagc ggatgctcgc ctacatcaag cgggtggacg
     1321 tggcccggct cgacgcggac tggccgcgcc gcgtggcggt cgagcgcgtg gccatcgaca
     1381 agccgtgggt cctcctcgag cgcgagaccg acggcacggt gccgctgctc gggctcctga
     1441 tggcgggccc gtccgcgccg aagaccgcgc ccgtgccgcc gccccgcgac cgcgccccga
     1501 cgggcgccgc cgcgccgccg cccgtcgtca cggtgggtgc gctcgcggtg gaggaaggct
     1561 tcgtgcgctt cgtggaccgc acgaccacgc cggccttcgt cgaggaggcc tcccgcatcg
     1621 cgctgaccgg ccgcgggctc ggcaccgcgc ccgacagccg cggccaggtc gcgatgaacg
     1681 ggcggctcac cggcggcgcg cccttcgaga tgaacggcac gctgggcgcg atcggcggcc
     1741 cgctcaacct cgaggtcgac ggcaagctca ccgacttccc gctcacccgt gtcaacccgt
     1801 acagcaacaa gctgatcggc tgggtcgcgc gccgcggcgc cttcggggcc accgtgcact
     1861 accgagtggt cgacaaccgg atcgaggcca ccaacgacct gctgatcggc cagcccgagt
     1921 acgcgccctc gcgcagcggc gacgaggtgc gcgagcgggt cggggtgccc ttcggcatgc
     1981 tcgtctccct gctcaagaac gtgcgcgggg agatccgcct ctcggtaccg gtctcgggcg
     2041 acctcgcctc acgccagttc gacctgagcg aggccttctg ggccgcgatg cgcgagaccg
     2101 cgatcagcgt gatggcgctg ccggtgagct gggtgggcaa gatcttctac accgaggacg
     2161 cgcgcatcga gaccgtgcag atctggccgg tctacttcga ggcgggcagc acccgcatcg
     2221 tgcgcgactt cgagcagcac gccaagcggc tggccacctt cctgcgcgag gcgcccgggg
     2281 tgaccctcgc cctgaaaccc gtccacaccg tggacgacat cgtggccctc aagcgcgagg
     2341 cggtgcgcca gcgcatcgac gcgggcgccc gcgagccggg tcagaccgcc gaggcggtcg
     2401 cggcgcgact cttcgcggaa cgcttcccgg ggcaggcgcc gccgcccggg ctcgacgcgc
     2461 tcgtgacgga gctggtgaag gccgagccga acccggacgc ggcggcccgc aacctcgcgg
     2521 agcagcgcat ggacatcacc cgcgcgcgcc tcgaagcctc gaagggcacc gccaacgcgg
     2581 cgcgcctgcg catcaccgag ggcgtggtcc cggtcgaagc ctccggccag ggccgcatcg
     2641 agttcgagat cgtcccctag acggggtcag gtcttacatt acgacaaatg taggaatgta
     2701 agacctgacc ccggatcaga acttggggtt gtcggtgcgg tcctcgaggg cggcgggacc
     2761 ctcggtgggc agggtctcgg cgtagttctg ggccgcgtcg atatcgcgcc cccacacctc
     2821 ccggggcagc tcgtagagga tctcgatccc gtgcccgtcc ggatccgaga tgtagacgct
     2881 gtgggtcata ccgtggttga tgcggcggtg gaacgtgaca ccgcggctct gcaggaactc
     2941 gagctgcttg agccacgact cccgatccgg ccaggcgatg gccacgtggt tgaggccgac
     3001 cttcggtccc atgaggctcc acgtctcggg cgagccctcc gccgccgggg cgccctcctt
     3061 gcgcggcacc tcggcgagcg ccaggtcgtg gtgggagaga tcgccgttgt cggccacgcc
     3121 gctgtagaag cgcatcttcg gccgcacctt ccccggctgc ggcttgagct ccgccacaca
     3181 gcggaagccc acgacctcgg tccagaagcg gtgcgagacc tcgatatccc gcacgttgag
     3241 cacgacgtgg ttcacgccgc gcggcgtctg ggcgctcccg acggcacgga catccgactc
     3301 ggtcttggcc atggctctcc ctccggcggc tagaacatcc cgttgttgtg ggccgccttc
     3361 tccggaatct cctggatcac gtcccgatca taccgcctac agccggctct cgtcgatctt
     3421 gaggccgagc cagtgacggc cgaccagctc gtagtgggcc tggcgggcct gcaggtgctg
     3481 gctcatcacg tggacgtcct ggaagtgccg ctggatcggg ctcgactcgt aggccgcggt
     3541 ggcgcccgcc atgttgtaga ccatgtccac gacggagacc gcggtgcgga tgccgtcggt
     3601 ggtggccagg cgcagcaccg cgcgctgctc cagggtgacc gcgccgtggg cgaccaccgc
     3661 gtcccagatc tcggccaccg cttcccgaag aaaagcgcgg ccggaccgca gccgggcctc
     3721 ggcgcggccc acctcggtct ggatcatcgg ctgatcgcgc agcagggcgt ccatggcccg
     3781 cggggtcttg gtggtagcca ggtccatgaa cgcggccagg cagctccggg cggtcccgag
     3841 ggccaccgcg gcgtccccgg aagcgaagac cagggtccgc gggagccggt agagcgggcc
     3901 ctgctccacc agcggcgcgg tcaccgactt gacggtgcgc tcctccggca cgaagacgtc
     3961 gtccaccgcg aagtgatgcg taccggtgcc gcgcatgccc cggacctgcc aggtgtcgag
     4021 cagctgggct tccgcgcgcg gcacgaagca gtagcgcatc tcgggctggc cgtcgtcgag
     4081 ccggggccgg ccccgctcgg tgacggtggt gtgcgcggcc agccacgagg cgtgccggca
     4141 gccggtgctg aagccctggc ggccggtgac ccggtagccc cccggcacca ccagggcctg
     4201 ggcgttggcc tgcggcgtat tggccaccac cgagcgcggg gtgtcgatcc agatggcgcg
     4261 cgccgccgcg gtctccatcc gcgccgcgta ggtcgcgaag atggacacct ggttggtcac
     4321 ccaggcggtg ctcgcgtcgg cccgcccgat ctcctcgatc acctggatgt agctcggcag
     4381 gtccatctcg aagccgccga gcgtccgcgg cagcccgagc cggaagaggc cggcgtcggc
     4441 catcgcctcg aagagcggac gcggcagctc gcgctgcgcg tccgtctccg gggcggacga
     4501 ccggatcagc ggcgcgagct tccgcgccgt ctcgagcggc gagagggtca ctggatcggc
     4561 gagtgcggcg tcgcggtcag gcgcgtcacc ttgatctgtt cgcggtcgat catggtgccg
     4621 acccgcgcgg agatctcgtt cttccagtag tcgctctggt agaccgcgtc gtagagccgc
     4681 ttgcgctccg cctcgctctc gaagcggcgg atccagacgt agaccgactg gtcctcctcg
     4741 cccacgaagc tgccgagaat gaccatgccc atcttgacct ggaacgggat gatctcctcc
     4801 tccatgcact tcacccactt ctcctgctgt ccgggacgga tgtggtactg cctgagctcg
     4861 aagaacatgc cggcctcctg ggtctcgtgt gggctgccgg ggcagcatac cgcgaatccg
     4921 ggcaggggta cacggcaccc gccggagcgt gtatgatccc tcgcgatggc ccctctcgtc
     4981 tgcggcggct gctgcgatgt gcgcgcggtc accgcggtca ccgcggtccc gctcgcggcg
     5041 gctcggcggc cgcggcgggc gccggatgga gcccatcgcg cggcggccgg cggccggcgc
     5101 gaggccgccg ccgggcgtcc ggtggccact ccgggcgtgc agggctactc cccgcccccg
     5161 ccgccggtca gcccgatcaa gggcctgatc gacttccaca cccacgcggc gcccgacatc
     5221 ttcggccgcg ccctcgacga cgacgagctg gccacgctgg ccgccacgcg ccagatggag
     5281 gcggtggtct tcaagaacca cgtgacccac accgccgacc gggccttcct cgtccgcaag
     5341 cacgtgccgg gcatcaaggt gttcggcggc atcacgctca accaggcggt gggcgggctc
     5401 aacccgcagg ccgtcgagtg gatgtggcgg atgcagggcg gcttcggccg cgtggtctgg
     5461 ttccccacct tcgacgccga caaccacgtc aagcacgccg gcaccgcgcc cgccggcatc
     5521 cgggtggtgg acgagcgcgg ccaggtcctg ccggcggcgc gcgcggtgct gaagatctgc
     5581 gcggcgcagc gcctggtggt gcagaccggg cacgcctcgc ccgacgaggc gctggccctg
     5641 atcgcggccg cgcgcgaggc gggctgcgag cgcatcgtgg tcacccacgc gcagttcgac
     5701 gtggtgggca tgagcctggc gcagatgaag aaggcggcgg cgctcggcgc caagctcgag
     5761 ctctgcgctc tcctcatgct ggcgggcccg acctccccgc tcgagttcat gcaccacacc
     5821 gcgcgggtgg aagtctcgga caccgcggcg cgcatcaagg cggtgggcgc ccagcacttc
     5881 gtgctcggca ccgacctggg ccaggcgggc aatcccacgc ccgccgacgg cctgcagatg
     5941 ctggtggccg gcctcatgaa ggaaggcatc acccgcgatc agatccagac catggggcgc
     6001 gagattcccg gcgcgctctt gatgggctga catgctctct tgcacgcgcg tctttcagct
     6061 gcgccgggcg ggggcgggga ggaggccctc cccggaacac gctgactcgc aaccctttac
     6121 cgg
//
LOCUS       DASUFA010000396         8458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_434736, whole genome shotgun sequence.
ACCESSION   DASUFA010000396 DASUFA010000000
VERSION     DASUFA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434736"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="VFX87_14380"
     CDS             complement(<1..1771)
                     /locus_tag="VFX87_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4996167.1"
                     /translation="MRATLPSVCPHDCPSQCALQVTVEAGRITDVTGDPGHPFTRGVI
                     CGKVHDYAERVYGPTRVRTPLRRVGPKGEGRFEPIGWDEAVDEIAHRWRRVIAQWGGE
                     AILPFSYGGTLGIVQNKAGHPLFHALGASRLDRTICISTAFAGWQGTLGTVTGNDSEQ
                     MVDSDLIVLWGINASHTHINAMTLVKQARRRGAFVVCVDPYRTPTARQADWHLMPRPG
                     TDGALALGVMHVLIGEGRVDQDYIQRATLGFTALAQHVKQYDPERVAAITGLAAPDVV
                     AFARRYGATRAAFIRVGIGLSRHDNGGMTCRTIACLPALTGAYAHPAGGALLGSSSAF
                     GLSNAVLERADLMPAPAPRVINMIQLGRALADPDLAPPVKALYVYNANPASIVPNQEL
                     VLRGLAREDLFTVVHEQHLTDTTDYADLVLPATTSMEHTDCYKSYGHFYVQLARPVIA
                     PEGEARSNWEVTRLLARAMGLRDPHFSKSEDDLIRETLASGDPSVEGITLERLEREHS
                     VRLRLGRPYLPFADGAPTPSGKVEFVSESLARAGLPALPTYTPLVEGPEHAELTRRYP
                     LQCIVPPNRFFLNSSFSQSELMRR"
     gene            1796..3055
                     /locus_tag="VFX87_14385"
     CDS             1796..3055
                     /locus_tag="VFX87_14385"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996168.1"
                     /translation="MREHPFQSPEARRLALLFAIVYFAQGMIYLPDQVVAIVFKERGL
                     TAGQLAAFTWIITIPWFVKPVYGLLSDFVPLFGTRRRSYFLIMSGVAALAAVAVSLMP
                     DASYALLATVIALLWLGVGFTDVLTDALMVENGQRMELTGAFQSVQWGALSVASILVG
                     VGGGYLAEHRAFTAAFALVACFPVVSFLMALLVVREAPARPSLEGFGETWSAVRAALG
                     RSDLWAVAGFTFFWAFSPSFGPAFFYYQTDVLGFSQTFIGVLSSLGAGMSIVGAWAYA
                     RLSQRFSLKRLIVWSIGAGVLGTLAYLVYRGPVSGIVITVVFGAIGMTTQLAFLDLAA
                     KACPRRAEATFFALLMSVYNIGMRSAEWTGANLYDWLGYAPLVLISTAFTAAAWLLVP
                     LVPIDAIQAGAQASSRSSRSTASLADS"
     gene            complement(3004..3414)
                     /locus_tag="VFX87_14390"
     CDS             complement(3004..3414)
                     /locus_tag="VFX87_14390"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX4996169.1"
                     /translation="MDPRRPFRGLGSARKTRRTLERGGTGPAAELNQTLGKWPRVRIT
                     PMFGRWGYFVGPRLFACFPLRAKDSDLWIRLAPEDQRRALAAGCSPHRRMSATGWVEC
                     RVEDPRDTGRAIRWLRRSYESAKEAVEREEREDA"
     gene            complement(3416..4195)
                     /locus_tag="VFX87_14395"
     CDS             complement(3416..4195)
                     /locus_tag="VFX87_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4996170.1"
                     /translation="MIPRLSGKVALVTGGATGIGRATVLALAREGAAVAVSYSKSKAA
                     AHETAKLAEQAGVRALPVQADVTRDAQVRAMVDEVARGLGGRDCLVNSAGWTRRVPHR
                     QLDALTDGIWDRVFATNLRGAFYCVRAAVPHMERRGGGAIVNITSVATFTGTGSSMAY
                     AASKAGLDALTKSLARALAPSRIRVNAVAPGLLATGFGGGLVTQDLLAQIGSLTPIGR
                     PVTVDEAAEAVVFLLANEALTGYTTIVDGGLTALGPTNPLS"
     gene            complement(4192..4953)
                     /gene="mce"
                     /locus_tag="VFX87_14400"
     CDS             complement(4192..4953)
                     /gene="mce"
                     /locus_tag="VFX87_14400"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX4996171.1"
                     /translation="MVTSIHHVGIAVASLSQAYGFWRDTLGLPLVREATVKEQGVRAA
                     LLAAGDSEIELLEPLGADSPIGKFLAKRGEGLHHLCFKTPDVAGDLSALHSKGVALLD
                     TAPRDGLAGRIGFLHPRACHGVLVELATPASREAHTESPVRFKRLVIASPAPHDTAKT
                     YQDLFGLPEVEVNGGPRTMLGWPGGSTLLMVPASEVGGMEGMIALSMVAPDMPPLVAR
                     LERARAGLLLGAGELTVEPQSSHGVHLHISRYHFP"
     gene            complement(5013..6095)
                     /locus_tag="VFX87_14405"
     CDS             complement(5013..6095)
                     /locus_tag="VFX87_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4996172.1"
                     /translation="MAEAATIRGFATVEGTAGYRTLRGARAGEGHFRTWQGLTVSSLG
                     IGTYLGAEDEATDRAYQASVRRALELGFNVVDSAINYRHQRSERNVGAVLRALAREVR
                     IAREDVLIATKGGFMPHPADAYVKAGIVKADEVVGDGHCIAPRFLADQLDRSRANLGI
                     ETIDVYYVHNPETQLAAVDRATFLARMRAAFEFLEGAVGDGKICWYGAATWTGFRQPA
                     TARDYLSLPELVGLAHEVAGDGHHFGMIQLPYNLAMTEAITVANQRVGPATVPIMQAA
                     EQLGVYVMTSASILQGKLARNLPPALAQVLEGLRTDAQRALQFVRSTPGVGTALCGMR
                     SLAHVEENAGLVAVPPVPSEKLFSRA"
     gene            complement(6095..7714)
                     /locus_tag="VFX87_14410"
     CDS             complement(6095..7714)
                     /locus_tag="VFX87_14410"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX4996173.1"
                     /translation="MTQLAGFLDVLLRGFGTVGLAAAVGGLVYLRLVLRPPAGAGGLV
                     AAARGRALTLVAAGALLAAGAELLLLLLVHPWALAEDSGRWPLREFLATEFGLAGLGR
                     VALSLAVAAAARWVRRAPDRALGWAVALGLGFLLMANAAWLAHAVSRLHDRGPLMVGT
                     VLHQLGAVVWVGGLLHLVGFVLLWRRAGGPAAEPLGVRVLARFSAVAIAALILVLAPG
                     LYLSISYVGGWGGLIGTGYGVMVLTKVALLGCALVLGGLNFLILRRADAGAGAIAARV
                     PAFIEAEVGLGLTLLLAAASLTSLPPSVDVVADRATPAEVAARFRPAMPRLSSPPVGQ
                     LLAAAAPIDDTLAKRQPEEYAWSEYNHHAAGFFVFAMGLLALLDQSGRARWARHWPLG
                     FLGLAAFLFVRNDPRAWPLGPAGFWESMVLPDVLQHRLVVLLVVALAVFEWMVRSARL
                     TRPGGRLVFPLLCASGGALLLTHSHAMFNLKTEFLTEVSHAPMGLLGVVMAWGRWLEV
                     RLPAADGRVPGWIWAACMTAIGLILLVYRET"
     gene            complement(7719..8105)
                     /locus_tag="VFX87_14415"
     CDS             complement(7719..8105)
                     /locus_tag="VFX87_14415"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX4996174.1"
                     /translation="MALAFRRAAAPALLLMACLTLAAPRAASPHAIVLESSPIHDAVL
                     PRAPQQVTLRFNSRIEKRFTRVTLALADRAPVAVAVPAGDAEADRLTIPLQPLGPGVY
                     VLRYRVLAVDGHITEGALRFTVGAAR"
     gene            complement(8111..>8458)
                     /locus_tag="VFX87_14420"
     CDS             complement(8111..>8458)
                     /locus_tag="VFX87_14420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4996175.1"
                     /translation="DTLLLEKGREVIGFAVCHLPPNSEAPHGSAYVKFLAIDSAHRKP
                     EYLQALLAGVEDLAAAAQLQRVVVPAYTYYWTAYQTLLERGYHVDFTMVRMKRGKQED
                     YEDAGDLVLDDWR"
BASE COUNT         1208 a         3045 c         3071 g         1134 t
ORIGIN      
        1 ggcggcgcat cagctcggac tggctgaacg acgagttgag aaagaagcgg ttgggcggca
       61 cgatgcactg cagcggatag cgccgcgtga gctcggcgtg ctccggacct tccaccaggg
      121 gtgtgtaggt gggcagcgcg ggcaggcccg cccgggccag cgactccgac acgaactcga
      181 ccttccccga cggagtcggc gctccatcgg cgaagggcag ataggggcgg cccaggcgga
      241 gccgcaccga gtgctcgcgc tcgagccgct ccagcgtgat gccctcgacc gacggatcgc
      301 ccgaggcgag cgtctcgcgg atgagatcgt cctcgctctt gctgaagtgc gggtcgcgga
      361 ggcccatcgc ccgcgcgagc agccgggtca cctcccagtt cgagcgcgct tcgccctccg
      421 gcgcgatcac cggccgggcg agctgcacgt agaagtggcc gtaggacttg tagcagtcgg
      481 tgtgctccat ggacgtggtg gccgggagca cgaggtcggc gtagtcggtg gtatcggtga
      541 ggtgctgctc gtgcaccacc gtgaagagat cctcgcgcgc gagcccgcgc agcaccagct
      601 cctggttggg cacgatcgag gccgggttcg cgttgtagac gtagagcgcc ttgaccggcg
      661 gcgccaggtc cggatcggcg agcgcgcgcc cgagctggat catgttgatc acccgcggcg
      721 ccggcgcggg catcaggtcc gcccgctcga gcacggcgtt ggagaggccg aacgcggagc
      781 tggagcccag cagggcgccg cctgcgggat gcgcgtaggc cccggtcagc gcgggcaggc
      841 aggcgatggt ccggcacgtc atgccgccgt tgtcgtggcg ggacagcccg atcccgaccc
      901 ggatgaacgc ggcgcgtgtg gccccgtagc ggcgggcgaa ggccacgacg tcgggggcgg
      961 cgagcccggt gatcgcggcc acgcgctcgg gatcgtactg cttgacgtgc tgggccagcg
     1021 cggtgaagcc gagggtggcg cgctggatgt agtcctggtc cacgcgcccc tcgccgatga
     1081 gcacgtgcat gacacccagg gccagggcgc cgtccgtccc cggccgcggc atcagatgcc
     1141 agtcggcctg ccgggcggtc ggagtccggt agggatccac gcagaccacg aaggcgccgc
     1201 gccggcgcgc ctgcttgacc agcgtcatcg cgttgatgtg ggtgtgcgag gcgttgatgc
     1261 cccagagcac gatcagatcc gagtccacca tctgctcgga gtcgttgccc gtcacggtgc
     1321 ccagggtgcc ctgccagccc gcgaaggcgg tgctgatgca gatggtgcgg tcgagccgcg
     1381 aggccccgag cgcgtggaag agcgggtgcc ccgccttgtt ctgcacgatc ccgagcgtgc
     1441 cgccatagga gaacggcagg atcgcctcgc cgccccactg ggcgatcacg cgccgccagc
     1501 ggtgggcgat ctcgtccacc gcctcgtccc agccgatcgg ctcgaagcgc ccctcgccct
     1561 tgggcccgac tcgacgaagg ggcgtccgca ctcttgtcgg cccgtagacg cgctcggcgt
     1621 agtcgtggac cttgccgcag atcaccccgc gggtgaaggg gtgcccggga tcgccggtga
     1681 cgtcggtgat gcgccccgcc tccaccgtga cctggagcgc gcactgcgac gggcaatcgt
     1741 gcggacagac ggacggcaac gtggctcgca tcgagaggct agattatcat gggccatgcg
     1801 cgagcacccc ttccagagcc ccgaggcccg ccggctcgcg ctgctcttcg cgatcgtcta
     1861 cttcgcccag ggcatgatct acctgcccga ccaggtcgtc gcgatcgtgt tcaaggagcg
     1921 cggcctcacc gccggccagc tcgccgcgtt cacctggatc atcacgatcc cgtggttcgt
     1981 caagccggtc tacgggctgc tgtcggactt cgtgccgctc ttcggcaccc ggcggcggag
     2041 ctacttcctg atcatgtccg gggtggccgc gctggccgcg gtcgcggtga gcctgatgcc
     2101 ggacgcgtcc tacgccctgc tcgccaccgt gatcgccctg ctctggctgg gggtgggctt
     2161 caccgacgtc ctgaccgacg cgctgatggt ggagaacggc cagcggatgg agctgaccgg
     2221 cgcgttccag tccgtgcagt ggggtgcgct gagcgtggcc tcgatcctgg tcggggtggg
     2281 cggcggctac ctcgccgagc accgcgcctt cacggccgcc ttcgcgctgg tcgcctgctt
     2341 cccggtcgtc tcgttcctga tggccctcct cgtggtgcgg gaggcgcccg cgcggccgag
     2401 cctcgaaggc ttcggcgaga cctggagcgc ggtccgcgcg gcgctcggcc ggagcgacct
     2461 gtgggcggtg gcgggcttca cgttcttctg ggcgttcagc ccgtcgttcg ggcccgcgtt
     2521 cttctactac cagaccgacg tcctcggctt ctcccagacg ttcatcggcg tgctctcctc
     2581 cctgggggcc ggcatgagca tcgtgggggc ctgggcctac gcacgcctgt cgcagcggtt
     2641 ctcgctgaaa cgcctgatcg tgtggtccat cggcgccggc gtgctgggca cgctcgccta
     2701 tctcgtctac cgggggccgg tgtcggggat cgtcatcacg gtggtcttcg gcgcgatcgg
     2761 catgaccacc cagctcgcgt tcctggacct ggcggccaaa gcctgcccgc gccgcgcgga
     2821 ggccacgttc ttcgcgctcc tgatgtcggt ctacaacatc gggatgcgca gcgcggagtg
     2881 gaccggcgcc aatctctacg actggctggg ctacgcgccg ctggtgctga tctcgaccgc
     2941 cttcaccgcg gcggcgtggc tcctggtgcc gctcgtgccc atcgacgcca tccaggccgg
     3001 cgctcaggcg tcctcgcgct cctcgcgctc gaccgcctcc ttggcggact cgtaggagcg
     3061 ccgcagccag cggatggccc gccccgtgtc gcgtgggtct tcgacgcggc actcgaccca
     3121 gcccgtcgcg ctcatgcgcc ggtggggcga acaccccgcc gcgagcgcgc gccgctgatc
     3181 ctccggcgcg agacggatcc agagatcgct gtccttggcg cggagcggga agcaggcgaa
     3241 caggcggggc cccacgaagt agccccagcg cccgaacatc ggggtgatcc ggacccgggg
     3301 ccatttgccg agggtctggt tcagctcggc ggccggcccg gtcccgccgc gctccagcgt
     3361 ccggcgggtt ttccgcgccg agccgaggcc gcggaacggc cggcggggat ccacgctagc
     3421 tcaggggatt cgtgggaccg agggcggtga gcccaccgtc cacgatcgtg gtgtagccgg
     3481 tcagggcctc gttggcgagc aggaacacca ccgcctccgc ggcctcgtcc acggtgacgg
     3541 ggcggccgat cggcgtgagg ctcccgatct gggcgaggag gtcctgcgtg acgaggccgc
     3601 cgccgaagcc ggtggcgagc agccccgggg ccaccgcgtt gacccggatg cgcgagggcg
     3661 ccagcgctcg ggccagcgat ttcgtgagcg cgtcgaggcc ggccttggac gcggcgtagg
     3721 ccatggacga gccggtaccg gtgaacgtcg ccaccgaggt gatgttgacg atggccccgc
     3781 cgccccgccg ctccatgtgg ggcaccgcgg cgcgcacgca gtagaaggcg ccgcgcaggt
     3841 tggtggcgaa gacgcggtcc cagatcccat cggtgagcgc gtcgagctgc cggtgcggga
     3901 cgcgccgcgt ccagcccgcg ctgttcacca ggcagtcgcg cccacccagc ccgcgcgcga
     3961 cctcgtcgac catggcccgc acctgggcgt cgcgcgtgac gtcggcctgg accggcaggg
     4021 cgcggacacc cgcttgctcg gcgagcttgg ccgtctcgtg ggccgccgcc ttcgacttgg
     4081 agtagctgac cgccaccgcg gccccttcgc gggccagcgc cagcaccgtc gcgcggccga
     4141 tgccggtggc gcccccggtg accagcgcga ccttcccgga aagcctgggg atcaggggaa
     4201 gtggtaccgg gagatgtgca gatgcacccc gtgggaggac tgcggctcca cggtcagctc
     4261 gcccgccccg agcagcagcc cggcccgggc tcgctcgagc cgcgcgacca ggggcggcat
     4321 gtccggcgcc accatggaca gcgcgatcat cccttccatc ccgcccacct cgctcgccgg
     4381 caccatgagg agcgtgctcc cgccgggcca gccgagcatg gtgcgcggtc cgccgttgac
     4441 ctcgacctcg ggcagaccga agaggtcctg gtaggtcttg gcggtgtcgt gcggtgcggg
     4501 cgacgcgatc acgagccgct tgaagcgcac cggcgactcc gtgtgggcct cacgcgacgc
     4561 gggggtggcc agctccacga gcacgccgtg gcaagccctg ggatgcaaga acccgatgcg
     4621 gccggcgagc ccgtcgcgcg gcgcggtgtc gagcaatgcc acgccctttg agtggagcgc
     4681 gctcagatcg cccgccacgt ccggggtctt gaagcagagg tggtggagcc cttcgccgcg
     4741 cttggccagg aacttcccga tcgggctgtc ggccccgagc ggctccagca gctcgatctc
     4801 gctgtcgccc gcggccagaa gcgccgcgcg cacgccctgt tccttgacgg tcgcctcgcg
     4861 cacgagcggt aggcccagcg tgtcccgcca gaagccgtag gcctgcgaga ggcttgccac
     4921 cgcgataccc acatgatgga tgctggtcac catggcatgg tcacgaagat cacgctagcc
     4981 gacgaggccc agattttcaa ggtctttcgc cctcaggccc gggagaagag cttctcgctc
     5041 ggcacggggg gcaccgcgac gagccccgcg ttctcctcga cgtgagccag gctccgcatc
     5101 ccgcagagcg cggtccccac cccgggcgtg gaccgcacga actggagcgc gcgctgcgcg
     5161 tccgtccgca gtccttccag cacctgagcg agggccggcg gcaggttccg ggccagcttg
     5221 ccctgcagga tagacgccga agtcatcacg tagacgccga gctgctcggc cgcctgcatg
     5281 atcgggacgg tggccgggcc gacgcgctgg ttggccaccg tgatcgcctc ggtcatggcg
     5341 aggttgtacg ggagctggat catcccgaag tggtgcccat cgcccgccac ctcgtgcgcc
     5401 aggccgacca gctcgggcag cgagagatag tcacgcgccg tcgcgggctg ccggaagccg
     5461 gtccacgtgg cggcgccgta ccagcagatc ttgccgtcgc ccaccgcgcc ttccaggaac
     5521 tcgaaggccg cccgcatgcg ggccaggaag gtggcccggt ccaccgcggc gagctgggtc
     5581 tccgggttgt gcacgtagta gacgtcgatg gtctcgatgc ccaggttggc gcggctgcgg
     5641 tcgagctggt cggcgaggaa gcgcggggcg atgcagtggc cgtcgcccac gacctcgtcg
     5701 gccttgacga tcccggcctt cacgtaggcg tccgcgggat ggggcatgaa gccgcccttg
     5761 gtcgcgatca ggacgtcctc gcgcgcgatc cgcacctccc gcgccagcgc gcggaggacc
     5821 gcgcccacgt tacgctcgct ccgctggtga cgatagttga tggcggagtc caccacgttg
     5881 aatcccagct cgagcgcccg ccgcaccgag gcctggtagg cgcggtcggt cgcctcgtcc
     5941 tccgccccca ggtaggtgcc gatcccgagc gaggagacgg tgaggccctg ccaggtccgg
     6001 aaatggccct cgccggcccg agcgccccgc agcgtgcggt agcccgcggt gccctccacc
     6061 gtcgcgaacc ctcgaatcgt cgccgcctcc gccatcatgt ctcccggtac accagcagga
     6121 tgaggccgat cgcggtcatg caggcggccc agatccagcc ggggacgcgc ccgtcggcag
     6181 cgggcagccg cacctcgagc cagcggcccc aggccatgac cacgccgagc agccccatgg
     6241 gcgcgtgaga gacctcggtg aggaactcgg tcttgagatt gaacatggcg tgcgagtgcg
     6301 tcaggagcag cgcgcctccc gacgcgcaga gcagcgggaa gaccaggcgc ccgccgggcc
     6361 gcgtgagccg cgccgagcgc accatccatt cgaacaccgc gagcgccacc accagcagca
     6421 ccaccagccg gtgctgcagc acgtcgggca ggaccatgct ctcccagaac ccggccgggc
     6481 ccagcggcca ggcccgcggg tcgttgcgca cgaacaggaa cgcggccaga ccgaggaagc
     6541 cgagcggcca gtgccgcgcc cagcgcgcgc ggcccgactg gtcgagcagc gcgagcagcc
     6601 ccatggcgaa cacgaagaac cccgccgcgt ggtgattgta ctcggaccac gcgtactctt
     6661 ccggctggcg cttggccagg gtgtcgtcga tcggggccgc cgcggccagg agctgcccga
     6721 ccgggggcga gctgagccgc ggcatcgcgg gccggaagcg ggcggccacc tcggccgggg
     6781 tggcgcgatc ggccaccacg tccaccgacg gcggcagcga ggtcagcgag gcggccgcga
     6841 gcagcagcgt gaggcccagc cccacctcgg cctcgatgaa agcgggcacg cgcgccgcga
     6901 tcgcccccgc ccccgcgtcg gcccggcgca ggatgaggaa attgaggccg cccagcacca
     6961 gcgcgcagcc cagcagcgcc accttggtca gcaccatgac cccgtagccg gtgccgatca
     7021 ggccgcccca cccgcccacg tagctgatgg acaggtagag gccgggggcc agtaccagga
     7081 tcagcgcggc gatggccacc gcggagaagc gcgcgagcac tcgcacgccc agcggctcgg
     7141 ccgccgggcc gcccgcgcgg cgccagagga gcacgaagcc gaccaggtgg aggagcccgc
     7201 cgacccagac caccgccccg agctgatgca gcacggtacc gaccatgagc gggccgcggt
     7261 cgtgcagccg actcaccgcg tgggcgagcc acgccgcgtt cgccatcagg aggaagccca
     7321 ggccgagcgc caccgcccag ccgagcgcac ggtcgggcgc gcggcggacc catcgcgcgg
     7381 ccgccgccac ggcgagggag agcgcgacgc ggccgagccc ggccaggccg aactcggtgg
     7441 cgagaaactc gcgcagcggc cagcgccccg agtcctcggc cagcgcccag ggatgcacga
     7501 gcagcaggag gagcagctcg gcgcccgccg cgagcagtgc ccccgcggcg acgagggtga
     7561 gggcccgccc gcgcgcggcg gccacgaggc ccccggcgcc ggccggcggc cgcagcacga
     7621 gccgcaggta gacgaggccg ccaacggccg cggcgagccc caccgtcccg aagccgcgca
     7681 gcaggacgtc gagaaaaccc gccagctgcg tcatgccgct agcgggcggc gcccaccgtg
     7741 aagcgcagcg cgccctcggt gatgtggccg tccaccgcga gcacgcgata ccgcaggacg
     7801 tagacgccgg ggcccagcgg ctggagcggg atcgtgagcc ggtccgcctc cgcgtcgccg
     7861 gcgggcaccg ccaccgccac cggcgcccgg tccgcgaggg ccagcgtcac ccgcgtgaag
     7921 cgcttctcga tccggctatt gaagcggagc gtcacctgct gcggggcccg cggcagcacc
     7981 gcgtcgtgga tcggggacga ctcgagcacg atggcgtgcg gggacgccgc gcggggcgcg
     8041 gcgagcgtga gacaggccat caacagcagg gccggcgcgg cggcccgtcg gaacgcgagc
     8101 gccacgcgga tcagcgccag tcgtccagca ccagatcgcc ggcgtcttcg tagtcctcct
     8161 gcttgccgcg cttcatgcgg accatggtga agtccacatg atagccgcgc tcgagcagcg
     8221 tctggtacgc ggtccagtag taagtatagg cgggcacgac gactcgctgg agctgggcgg
     8281 cggccgccag atcttccacg ccggcgagca gggcctgcag gtactcgggc ttgcgatggg
     8341 cgctgtcgat ggccaggaac ttgacgtacg cgctgccgtg aggcgcctcg ctgttgggcg
     8401 gcaggtggca gaccgcgaag ccgatcacct cgcggccctt ctcgagcagg agcgtgtc
//
LOCUS       DASUFA010000397         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1479138, whole genome shotgun sequence.
ACCESSION   DASUFA010000397 DASUFA010000000
VERSION     DASUFA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479138"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFX87_14425"
     CDS             <1..207
                     /locus_tag="VFX87_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996176.1"
                     /translation="VAVFAVCATTEDEARRLARSRDLFIVRLYTGRPAPYPSVEEAER
                     YPYNAQELAIAQHAARRSVVGTPE"
     gene            208..366
                     /locus_tag="VFX87_14430"
     CDS             208..366
                     /locus_tag="VFX87_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996177.1"
                     /translation="MRERLLAMVADYQADELVVVTITHDFKARLRSYELLAEAFDLTG
                     AGDKEMTP"
     gene            363..1166
                     /locus_tag="VFX87_14435"
     CDS             363..1166
                     /locus_tag="VFX87_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX4996178.1"
                     /translation="MTMRLSLHTWTLDTTPLPDALAVIKKTGWDAVELRRLDFERAAQ
                     AGQSAEQVLALVRASGLPVACVGVELGWVWATGEERARLLRVFAEQAARARALGCATI
                     MSPVDRGRGDVGEAARSIREVGDIAREHGVRLAVEFNSQCEQLNALGPMREVMARAGH
                     PQTGLLLDTYHLGRTGASPRDIEDVRPEEIAYVQYSDVPRTGLEPGKALDRLPPGQGS
                     VPFKEFFAAFARQGYAGYASYEAPNPAAWKRPAEDVAREALAATRAVLP"
     gene            1181..>2177
                     /locus_tag="VFX87_14440"
     CDS             1181..>2177
                     /locus_tag="VFX87_14440"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4996179.1"
                     /translation="MRYRKRAMIVAAQPEATEAGAEILKAGGNAVDAAMACAFVQGVV
                     DPLMCGIAGFGSCGIAMLADGFHGYIDFHAPAPLAVRPDMWADRIESEARDGYGFILR
                     GRVNDIGYQAICAPASLRAYRDAHREHGRLPWEQILEPAIAWAEGGFTVRPHVGSWWA
                     EDGQMGRAANHERLAFTPASRALYCRPDGTPKRVGDRVVNRDLGQTLRLIAKHGDEVF
                     YEGEIAHAIVEDMRRHDGLLGAEDLRGWRTTRNAPLWSSYRRYRVSSNRPPGGGVMLL
                     EMLNILEHFPLASLEHNSAEYVRLVAEAMKRATIDKDRHVGDPRFVEVPVERLTGK"
BASE COUNT          311 a          767 c          817 g          282 t
ORIGIN      
        1 gtggcggtct tcgcggtgtg cgcgaccacg gaggacgaag cgcggcggct cgcgcggagc
       61 cgggacctct tcatcgtgcg cctctacacc ggccggcccg ccccgtaccc ctcggtggag
      121 gaggcggagc gctatcccta caacgcccag gagctggcca tcgcccagca cgcggcccgg
      181 cgcagcgtgg tgggcacgcc ggagtaggtg cgggagcggc tgctcgccat ggtcgccgac
      241 taccaggccg acgagctggt ggtggtgacg atcacgcacg atttcaaggc ccggctgcgc
      301 tcctacgagc tcctggccga ggccttcgac ctgaccgggg ccggtgacaa ggagatgacc
      361 ccatgaccat gcgcctgtcg ctgcacacgt ggacgctcga tactactccg ctgccagacg
      421 cgctcgcggt catcaagaag acgggctggg acgcggtcga gctgcgccgc ctcgacttcg
      481 agcgcgcggc ccaggccggc cagtccgccg agcaggtgct ggccctggtg cgggcctccg
      541 gcctcccggt ggcctgcgtg ggagtggagc tcggctgggt gtgggccacc ggcgaggagc
      601 gggcgcggct cctccgcgtg ttcgccgagc aggccgcgcg cgcccgggcc ctcggctgtg
      661 ccacgatcat gagcccggtg gatcgcggcc gcggcgacgt gggagaagcc gcgcgcagca
      721 tccgcgaggt gggcgacatc gcgcgcgagc acggggtgcg cctggccgtc gagttcaact
      781 cccagtgcga gcagctcaac gcgctcggcc cgatgcgcga ggtgatggcg cgggcgggcc
      841 atccccagac cgggctgctg ctcgacacct accatctcgg ccgcaccggc gcttcaccgc
      901 gcgacatcga ggacgtgcgg cccgaggaga tcgcctacgt gcagtacagc gacgtgccgc
      961 gcaccggcct cgagccgggc aaggcgctcg atcgcctgcc gccggggcag gggagcgtgc
     1021 cgttcaagga gttcttcgcc gcgttcgccc gccagggcta cgccggctac gcgagctacg
     1081 aggcgcccaa ccccgccgcc tggaaacgcc cggccgagga cgtggcgcgc gaggcgctgg
     1141 ccgccacacg cgcggtgctg ccctagggac aggagaatcc atgcgatacc gcaagcgcgc
     1201 gatgatcgtg gcggcccagc cggaggccac cgaggcgggg gcggagatcc tgaaggcggg
     1261 cggcaacgcg gtggacgccg cgatggcctg cgccttcgtg cagggcgtgg tggacccgct
     1321 gatgtgcggc atcgcgggct tcgggagctg cggcatcgcg atgctcgccg acggcttcca
     1381 cggctacatc gacttccacg cccccgcccc gctcgcggtg cgccccgaca tgtgggccga
     1441 ccgcatcgag tcggaggcgc gcgacggcta cggcttcatc ctgcgcggcc gggtgaacga
     1501 catcggctac caggccatct gtgcaccggc gagcctgcgg gcctaccgcg acgcgcatcg
     1561 cgagcacggg cggctgccct gggagcagat cctggagccg gcgatcgcgt gggcggaggg
     1621 cggcttcacg gtgcgcccgc acgtggggtc ctggtgggcg gaggacgggc agatgggccg
     1681 cgccgcgaac cacgagcgcc tggcgttcac gccggcctca cgggcgctct actgccggcc
     1741 ggatggcacc cccaagcgcg tgggtgaccg cgtggtgaat cgcgatctcg gccagacgct
     1801 gcggctgatc gccaagcacg gcgacgaggt gttctacgag ggcgagatcg cgcacgccat
     1861 cgtggaggac atgcgccggc acgacgggct cctcggcgcg gaggacctcc gcggctggcg
     1921 caccacgcgc aacgccccgc tctggagctc ctaccgccgc taccgggtat ccagcaaccg
     1981 gccgcccggc ggcggggtga tgctgctcga gatgctgaac atcctggaac atttcccgct
     2041 cgcctcgctc gagcacaact cggccgagta cgtgcggctg gtggccgagg ccatgaagcg
     2101 cgcgacgatc gacaaggacc gccacgtggg cgatccgcgg ttcgtggagg tgccggtgga
     2161 gcggctgacc ggcaagc
//
LOCUS       DASUFA010000398         7232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1687906, whole genome shotgun sequence.
ACCESSION   DASUFA010000398 DASUFA010000000
VERSION     DASUFA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687906"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1144
                     /locus_tag="VFX87_14445"
     CDS             92..1144
                     /locus_tag="VFX87_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4996180.1"
                     /translation="MESAFARSPFIAIWEMTRACALACVHCRAAAIPRRDPGELTTDE
                     AKALLDRIAAFGDPPPLVVFTGGDPMRRPDVTELVAHGTRRGLVVSLTPSGTATVTED
                     RLRALTDAGLARLAVSLDGAWADAHDAFRRVRGSYRNTLRIIERARSLGLALQINTTV
                     CRRTVGELPALVDQVTGFGALLWALFFLIPVGRAQADQALTAGEVEAVLQWAADLQAR
                     VPFGIKTTEAPQYHRVLGGAHPFGSRPRMRAGRAVTDGNGFVFVDHLGNICPSGFLPL
                     PTGNVRRDDLVAVYREHPLFVALRDPARLGGRCGRCEWRARCGGSRARAYALHGDPLA
                     EDPGCAYEPARREADP"
     gene            1141..1428
                     /locus_tag="VFX87_14450"
     CDS             1141..1428
                     /locus_tag="VFX87_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX4996181.1"
                     /translation="MSIGRDQVTQALREVFDPELGMSVVDLGLIYEVQIDGGHVRITM
                     TLTTQGCPLHDSMTEWVRQAVGKVPGVHEVDVIITFEPAWTPERIGRTVVP"
     gene            1696..2655
                     /gene="nirK"
                     /locus_tag="VFX87_14455"
                     /pseudo
     CDS             1696..2655
                     /gene="nirK"
                     /locus_tag="VFX87_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
     gene            2787..3554
                     /locus_tag="VFX87_14460"
     CDS             2787..3554
                     /locus_tag="VFX87_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX4996182.1"
                     /translation="MGITASVARIRWRRWTGATLLVVALGPAPAWAGRPLDTEDTGTV
                     EPGKAELEVSGDFARNPEDRTWFAKGVLSLGVLPRLEIRIESMLLALEPDEGRGRGGI
                     GDSLVGLKHRLLDEAAAIPAVLGALTVRLPTGDEDRGLGAGEVDVGLLAVASKTFGPT
                     TLAWNGGYTFVTRDRELEFWTLAAAVEYRATAAWSLLGEVVSALASRGGPDTVVVRAG
                     TTYAVHPRVRLDAGIGFGVTRRSPDVLVTFGVTLALF"
     gene            complement(3577..5259)
                     /locus_tag="VFX87_14465"
     CDS             complement(3577..5259)
                     /locus_tag="VFX87_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX4996183.1"
                     /translation="MSDQEPIEEIRQARQRWENETARATAPGADAPHGSQHDPDLPVQ
                     AVYTPAEVADRDIDYLRDLGFPGQYPFTRGIYPSMYRGRLWTMRQYAGFGTARETNQR
                     FRFMLERGMTGINVAFDLPTQHGYDSDDPRARGEVGNVGVPVSSLRDLEILFDGIPLA
                     EVSPANAINAPAAVILAMYVALAERQGLPRARLSGSTQNDILKEFIARGTYIFPPRPS
                     LRLVTDVIEYCAEHLPRWNFINVCGYHMREAGSTLVQEVAFALADAVTYVQAAVARGI
                     AVDRFAPRFAFNFTAGTNLFEEAAKFRATRRLWARLMRERFGAKQPASWAFRTGAGSG
                     ASQLTAQQPENNLIRVTLNALAAILGGAQSLHTAAYDEALALPTERSVELALRTQQVL
                     AYEAGLTEVIDPLAGSYYVEALTTEIEARTRALMDEIERRGGVIAAIESGWTQQQIAD
                     ASYEHQRLVERGERVIVGVNRFVDEQPLHVDIHRHLEAVAAERAAALGVLREERDPRR
                     VARALDGLRAVAAGDGNVMPALVEAVKAYATIGEICGTLRELFGEYRAAQVY"
     gene            complement(5333..6472)
                     /locus_tag="VFX87_14470"
     CDS             complement(5333..6472)
                     /locus_tag="VFX87_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4996184.1"
                     /translation="MNRRDVLLTALAGIAGLLRGSRAAAGPAPAGPAGTRVVVTKEML
                     GAGMVSSGVALPAIPPVTGSRRSPVPSWGKPLPYPIVIADRRNNRLIEVTPDKRIVWE
                     FPSPDLGIYRGNEDVNFSPDGRLLAVSEEDNYDIHIVDYDKRALVWTYGAPDVKGSAP
                     GLFNYPDDAHLLEDGMFITADIRNCRVLIIDPRTNQVVTEWGKPGACRHEPPRHLGYP
                     NGVTPLDNGDILVTEIQGAWISRITRAGQVAWSVQAPRVRYPSDAFPTGDGQVIVADF
                     SKPGRVVIFDPASRRVTWEYEAKAGEKMLDHPSLARELPGTGDIIVADDLRERVVVID
                     RKTKDFIWQYGATGQKGHAPGYLNYPDGFDVDVFRDWKGALGVKP"
     gene            complement(6498..>7232)
                     /locus_tag="VFX87_14475"
     CDS             complement(6498..>7232)
                     /locus_tag="VFX87_14475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4996185.1"
                     /translation="LRGHAVTVLERASEPGGQVLLARLVPGRADLAGLVTYLAGAATR
                     AGALIHCGVEATVEAVVARRPDVVVLATGARPGVPGVPGIEQSPAVDAFTVLGRAAGH
                     VKRALVMGGGMLGVGVAHVLAERGGEVVIVEPGGELAGELGVRPRWQHVANLRDRRNV
                     TILLGATVEELYPDGALVRRAGEDVKLRDLDLVVPTRPMVPVSEVGDALKALPGGPAI
                     FDIGDCVVPRTAFEAMQDAAAIGHRL"
BASE COUNT         1005 a         2605 c         2565 g         1057 t
ORIGIN      
        1 ctcggacggg tcctggggtg cttgcacgcg ggagctcgga aatggccccc gcacgcggcc
       61 ggccgcgtgc tgccgaaggg gacgtgacgt ggtggagtct gccttcgcgc ggagcccgtt
      121 catcgccatc tgggagatga cgcgggcctg tgcgctggcc tgcgtgcact gccgggccgc
      181 ggccatcccg cgccgcgatc ccggcgagct caccaccgac gaggccaagg cgctcctgga
      241 ccggatcgcg gccttcgggg atccgccgcc gctggtggtc ttcaccggag gcgacccgat
      301 gcggcgcccc gacgtcaccg agctggtggc ccacggcacc cgccgcggcc tggtggtctc
      361 gctgaccccg agcggaaccg cgaccgtcac cgaggatcgg ctccgggcat tgacggacgc
      421 cggactggcg cgcctcgcgg tgagcctcga cggcgcctgg gcggacgccc acgacgcgtt
      481 ccgtcgcgtg cgcggctcgt accgcaacac gctccggatc atcgagcgcg cgcgctcgct
      541 cgggctcgcg ctgcagatca acaccaccgt ctgccgccgg accgtcgggg agctgcccgc
      601 gctggtcgac caggtgaccg gcttcggcgc gctcctctgg gcgctcttct tcctcatccc
      661 ggtggggcgc gcccaggccg atcaggccct caccgccgga gaggtcgagg cggtgctcca
      721 gtgggcggcg gacctacagg ctcgcgtccc cttcgggatc aagacgacgg aggcgccgca
      781 gtaccatcgc gtgctcgggg gtgcacaccc gtttggctcg cggccacgca tgcgcgcggg
      841 gcgcgcggtc accgatggga acgggttcgt gttcgtggat catctcggta acatctgccc
      901 gagcgggttc ctgcccctgc cgaccggcaa tgtgcggcgc gacgatctcg tcgcggtcta
      961 ccgcgagcac ccgctcttcg tcgcgctgcg cgacccggcg cggctcggcg gacgctgcgg
     1021 gcgctgcgag tggcgcgcgc gctgtggcgg cagccgcgcg cgagcctatg cccttcacgg
     1081 cgatccgctg gcggaggatc cgggctgcgc gtatgagccg gcccgccggg aggccgaccc
     1141 gtgagcatcg gccgggacca ggtcacgcag gcgctccgag aggtcttcga tccggagctc
     1201 gggatgtccg ttgtggatct cggcctgatc tacgaggtgc agatcgacgg cgggcacgtg
     1261 cgaatcacca tgaccctgac gacgcagggc tgcccgctcc acgactcgat gacggagtgg
     1321 gtccggcagg cggtcgggaa ggtccccggc gtccacgagg tcgacgtgat catcaccttc
     1381 gagcctgcct ggacccccga gcgcatcggc cggaccgtgg tgccgtagcc tgcccccgcg
     1441 gtagcggtgg cggcgcccca tcgtgaggcg ccgctgcccc gcccgctccc cggttgccct
     1501 atgagcggcg tcataacggt tcgcgtgggc ccggccgcat cctattgaca tgccgagcgg
     1561 atctcgagac gtgggcccgg cggcacctgc cttgccgtct cggcgagtag cgcaaacaca
     1621 tcatcaagga ggcgagcgat gagacggcga ctgggaatcg tggcgacggc ggccgcactg
     1681 ctgatcgcgg gcgcaatgat ctggcagagt caggcgcgcg acaaggccac cgagcccgcc
     1741 gcgctgccgg aggtgaaggc ggtcctgacc tcggcgccgc tcgtgcctcc gccggtggac
     1801 cggccgggca acgcgaaggt gatcgtcagc ctcgagacca ccgaggtgaa gggcgcgctg
     1861 gccgacggcg tgcagtacac gttctggacc ttcggcggga ccgtgcccgg cccgttcgtc
     1921 cgcgtgcgcg tgggcgacgt ggtgcagatc cgcctcaaga acgccgagaa gagcatgcac
     1981 ccgcactcca tcgacctgca cgcggtgacc ggtccgggcg gcggggcggc ggtgacccag
     2041 ctcggcccgg gccaggaggg cgccttcgag ttcaaggcgc tcaatcccgg gctctacgtc
     2101 tatcactgcg cgacgccgag cgtgcccgag cacatcgcca acggcatgta cgggctgatc
     2161 ctggtggagc ccgagaaggg gctgccccgc gtggaccgcg agtactacgt catgcagggc
     2221 gagttctaca cgaagggcaa gaccctggcg cccgggctgc agccgatcga tccggccaag
     2281 ctcacgtcgg agcggccgga gtacgtggta ttcaacggca agatgggcgc gctcctgggc
     2341 gacggcgcgg tcaaggccaa cgtgggcgag accgtgcggc tcttcgtggg caacggcggc
     2401 ccgaacctga tctcctcctt ccacgtgatc ggggagatct tcgacagcgt ctacaccgaa
     2461 ggcgcggtgg gcggcaacgc accggccaag aacgtgcaga ccacgctgat cccggcgggc
     2521 ggcgcggcga tcgtcgagct gcaggtccag gtgcccggcc gcttcctgct ggtagatcac
     2581 agcatcgtgc gggcgatgga gaagggggcc ctcggagtcc tggaggtggc gggcgccgag
     2641 cagcccggta tcttcaggac gctgatcccg ggcacccacg gcacgggcgg gcactagccc
     2701 cggccggctg ccggcatccc cgacctcctc accctgccct ctccccctca cgggggagag
     2761 ggtttgtttc gagtatgatc ggcgccgtgg ggatcacggc gtcggtggcg cggatccgat
     2821 ggcggcggtg gaccggagcg acgttgctcg tcgtggcgct gggacctgct ccggcatggg
     2881 ccgggcgccc tctcgacacg gaagataccg gcaccgtcga gccgggcaag gcagagctcg
     2941 aggtctccgg cgactttgcg cggaaccccg aggaccgcac ctggttcgca aagggcgtgc
     3001 tgagcctggg tgtcctgccg cggctcgaga tccgcatcga gtccatgctc ctcgctctcg
     3061 agccggacga ggggcgtgga cggggaggga tcggcgacag cctcgtgggg ctcaagcacc
     3121 gcctgctcga cgaggccgcc gcgatcccgg ccgtgctggg cgctctcacc gtcaggctgc
     3181 ccaccggcga cgaggaccgc gggctcgggg ccggcgaggt ggacgtgggg ctgctcgccg
     3241 tcgccagcaa gacgttcggc cccacgaccc tggcgtggaa cggcgggtac accttcgtca
     3301 ctcgcgaccg ggagctggaa ttctggaccc tcgcggccgc ggtcgagtac cgcgccaccg
     3361 ccgcgtggtc gctcctcggc gaagtcgtga gcgccttggc cagtcgcggg ggtccggata
     3421 cggtcgtcgt ccgggccggc accacgtacg ccgtccatcc gagggtccgg ctggacgccg
     3481 gcattggctt cggcgtgacc cgccggagcc cggacgtcct cgtcacgttc ggcgtgacgc
     3541 tcgcgctctt ctgagcggat acccccgcgc cgcggctcaa tacacctgcg cggcccgata
     3601 ctccccgaac agctcgcgca gcgtcccgca gatctcgccg atcgtcgcgt aggccttcac
     3661 cgcttccacg agcgccggca tgacgttgcc gtcaccggcg gcgaccgccc ggaggccgtc
     3721 gagcgcacgg gccacccgac gggggtcccg ctcctcccgc agcactccga gcgccgccgc
     3781 ccgctcggcg gcgaccgcct cgaggtggcg atggatgtcc acgtgcagcg gctgctcatc
     3841 gacgaaccgg ttcaccccga cgatcacccg ctctccgcgc tcgacgagcc gctggtgctc
     3901 gtacgaggca tcggcgatct gctgctgcgt ccagccggac tcgatggcgg cgatcacgcc
     3961 gccccgccgc tcgatctcgt ccatgagcgc gcgggtgcgc gcctcgatct cggtcgtcag
     4021 cgcctccacg tagtaggatc ccgcgagcgg gtcgatcacc tcggtcagac ccgcctcgta
     4081 ggccagcacc tgctgggtcc gcagggccag ctcgaccgat cgctcggtcg gcaatgcgag
     4141 ggcctcgtcg tacgcggccg tgtgcagcga ctgggctccg cccaggatgg cagccagcgc
     4201 gttgagcgtc acccggatga ggttgttctc gggctgctgc gcggtgagct ggctggcgcc
     4261 ggagccggct ccggtccgga acgcccagct cgccggctgc ttggcgccga agcgctcgcg
     4321 catgagccgc gcccacaggc gacgcgtggc ccggaacttc gccgcctcct cgaagaggtt
     4381 ggtgccggcg gtgaagttga aggcgaagcg cggggcgaag cggtccacgg ctatcccgcg
     4441 cgcgacggcc gcctgcacgt aggtgaccgc gtcggccagg gcgaaggcca cctcctgcac
     4501 cagcgtcgat cccgcctccc gcatgtgata gccgcagaca ttgatgaagt tccagcgcgg
     4561 caggtgctcg gcgcagtact cgatcacgtc cgtcaccagg cggagcgagg gccggggcgg
     4621 gaagatgtag gtgccgcggg cgatgaactc cttcaggata tcgttctggg tactgccgga
     4681 gagccgcgcc cgcgggagtc cctggcgctc ggccagcgcc acgtacatcg ccaggatcac
     4741 ggcggccggc gcattgatgg cgttggcagg gctgacctcc gccagcggga taccgtcgaa
     4801 gagaatctcg aggtcgcgga gcgagctcac cggcacaccc acgttgccca cttctccccg
     4861 cgcccgggga tcgtccgaat cgtagccgtg ctgggtgggc aggtcgaacg cgacgttgat
     4921 cccggtcatg ccccgctcga gcatgaaccg gaaccgctgg ttcgtctccc gcgccgtccc
     4981 gaaccccgcg tactggcgca tcgtccagag ccggccacgg tacatcgacg gatagatccc
     5041 gcgcgtgaat ggatactgac ccgggaagcc gaggtcccgg aggtagtcga tgtcccgatc
     5101 ggcgacctcg gcgggggtgt agacggcctg caccggcagg tccggatcgt gctgagaccc
     5161 atgcggcgcg tcggcgcccg gggccgtggc gcgcgcggtc tcgttctccc agcgctgccg
     5221 ggcctgccgg atctcctcga tcggctcctg gtcgctcatc gcgcgttctc ccccggggcg
     5281 gtcaggcccg atggccgacc gccgccgcgt cctgcacggc gccggcctcc cctcagggct
     5341 tgaccccgag ggcgcccttc cagtcgcgga atacgtcgac gtcgaatccg tccgggtagt
     5401 tcaggtagcc gggagcgtgc cccttctggc cggtggcccc gtactgccag atgaagtcct
     5461 tggtctttcg gtctatcacg acgacccgct cccgcaggtc gtcggccacg atgatgtcac
     5521 ccgtgcccgg cagctcccgg gcgagcgacg gatgatcgag catcttctcc cccgccttcg
     5581 cctcgtattc ccaggtgacc cgtcggctgg cgggatcgaa gatgacgacc cgacccggct
     5641 tggagaagtc ggccacgatc acctgcccgt ccccggtcgg gaaggcgtcc gacggatagc
     5701 ggacgcgcgg ggcctgtacg ctccaggcga cctggcccgc gcgcgtgatg cgcgagatcc
     5761 aggccccctg gatctcggtc accagaatgt cgccgttgtc gagcggggtc accccgttcg
     5821 ggtagccgag atggcgggga ggctcgtggc ggcacgcccc gggcttgccc cactcggtca
     5881 cgacctggtt cgtgcgcgga tcgatgatga gcacgcggca attgcggatg tccgcggtga
     5941 tgaacatgcc gtcctcgagc aggtgggcgt cgtcggggta gttgaacagg cccggcgcgc
     6001 tgcccttgac gtccggcgcc ccgtacgtcc agacgagggc gcgcttgtcg tagtccacga
     6061 tgtggatgtc gtagttgtcc tcctcgctca ccgcgagcag gcgcccgtcg ggcgagaagt
     6121 tcacatcctc gttgccgcgg tagatcccga ggtcgggcga cgggaattcc cacacgatgc
     6181 gcttgtccgg cgtgacctct atcaagcggt tgttgcggcg gtcggcgatg acgatggggt
     6241 acggcagcgg cttgccccac gacggcaccg gcgagcggcg ggagccggtc accggcggga
     6301 tcgccgggag cgcgacaccg ctcgatacca tgccggcgcc gagcatctcc ttcgtcacga
     6361 ccacacgtgt gccggccgga ccggcgggag ccggcccggc cgcggcccgc gagccccgga
     6421 ggagcccggc gatgccggcc agcgccgtca ggagcacgtc acgtcgattc atggcatcca
     6481 actcccttcg gggccggtca gagacgatgg ccgatggccg ccgcgtcctg catcgcctcg
     6541 aaggcggttc gcggcaccac gcagtcaccg atgtcgaaga tggcgggccc cccgggcaac
     6601 gccttgagcg cgtcaccgac ctccgagacc ggcaccatcg gcctcgtcgg caccacgagg
     6661 tccaggtcgc ggagcttcac gtcttccccc gcccgcctca ccagcgcgcc gtccggatag
     6721 agctcctcca ccgtggcgcc gagcaggatc gtcacgttcc ggcggtcgcg caggttggcc
     6781 acgtgctgcc accgcggtct gactcccagc tcgcccgcga gctccccgcc gggctccacg
     6841 atcacgacct ccccaccgcg ctccgcgagg acgtgggcca ccccgacgcc cagcatcccc
     6901 ccgcccatga cgagcgcccg cttcacgtga ccggccgccc gtccgaggac cgtgaacgcg
     6961 tcgaccgccg ggctctgctc gatgcccggg acgcccggga cgccgggacg cgccccggtg
     7021 gcgagcacga ccacgtccgg tcgccgggcg acgacggcct ccaccgttgc ctcgacgccg
     7081 caatggatca gggcgccggc gcgggtcgct gcccccgcga gataggtcac cagtcccgcc
     7141 aggtccgcgc ggccggggac gaggcgggcc agcaagacct gcccgccggg ctcgctcgcc
     7201 cgctcgagca ccgtcaccgc gtgcccacgg ag
//
LOCUS       DASUFA010000399         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1239498, whole genome shotgun sequence.
ACCESSION   DASUFA010000399 DASUFA010000000
VERSION     DASUFA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239498"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1878
                     /locus_tag="VFX87_14480"
     CDS             343..1878
                     /locus_tag="VFX87_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX4996186.1"
                     /translation="MRIQLDRGTGGAGRASLSRQIQLHFERLISQGLLAAGMKLPASR
                     ELARELGVNRTTVALAYDELVAGGWARAHVGQGTFVADRVPATASITRPTAPRLDWTP
                     FLSKGAQVIAADSRRRRGYSQVPQPGPGAISFAGGMPDSGLFPTDAFRRVLNRVIREE
                     GRELLQYYPGRGYPPLREFLAGYLLRFGLEVRPQEILIVNGSQQGFDLVARTLLDPGD
                     FVAIEEPSYPRAMQVFRAFGAQLLPVPMRDGGLDPEHLDRLLERQSPKLLYCQPSAHN
                     PTGLTMRPEARKRLLEVAARHRLPIMEDGFDGSLFFGPQPPAPLKALDVSGLVIYIGT
                     FSKILFPGLRLGWIVAAPELIDRLEVAKDLADIHTSPLIQAAVYHFCRQRLLDRHQAR
                     VLREYGRRRAALLASLARRMPAGVTWTENEGGFSLLVTLPAGLDAAPLLERAAARGVI
                     FTPGNAFFVDGGGERTLRLSFSALPLAQIDEGVKRLAEIIRDVQRQPEPVGLVAAPAV
                     PLV"
     gene            1889..>2341
                     /locus_tag="VFX87_14485"
     CDS             1889..>2341
                     /locus_tag="VFX87_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX4996187.1"
                     /translation="MDFHTLAARGGRDVPSASRPLTPPIYQTNVYVFEDMDTVESVWE
                     SKKAGFVYGRYGTGNHAMLEDLVAALEGAETAVACASGMGATTALLLGLFAPGDHLVS
                     ARDLYGSTAAFLGDEGRRLGVETDFVDATDIGAIVKALRPTTRAVFVEA"
BASE COUNT          332 a          815 c          825 g          369 t
ORIGIN      
        1 aggtcgtagc tcgagtagaa gcgccggtac accaggttga cccggatgcc ttcccaggcc
       61 agcgattgct tgatggcttt cagatagggg agcaccgagg gctcgtcgtc cagcagacgg
      121 cccttcttgt tccacatcga ctccagcacc accacacccg gcatcggtcg atcctcgaag
      181 cggtcggtct tcggccctga tctcaccccg gtgggagccc agggacccct gggtgcgcca
      241 tgatatcact ggtccggctc atgaggattg gatgatgttg gaatcatcca attggacgtt
      301 tacaggcagc caaacaacaa gataatggat gatactcagg ccatgagaat tcaactggac
      361 cgggggaccg gaggggcggg cagggcatcc ctgtcgcgcc agatccagct ccacttcgag
      421 cgactgatca gccagggcct gctcgcggcg gggatgaagt tgccggccag ccgtgagctg
      481 gcccgcgagc tcggggtcaa ccgcacgacg gtggccctgg cctacgacga gctggtggcg
      541 ggcggctggg cccgggccca cgtggggcag gggacgtttg tcgccgatcg ggtgcccgcc
      601 accgccagca tcacgcgtcc caccgccccg cggctcgact ggacgccgtt cctgtccaag
      661 ggcgcgcagg tgatcgcggc ggacagccgc cgccggcgcg gctacagcca ggtgccgcag
      721 cccgggccgg gcgccatctc gttcgcgggc gggatgcccg acagcgggct cttccccacc
      781 gatgccttcc ggcgcgtgct caatcgcgtg atccgcgagg aagggcggga gctgctgcag
      841 tactacccgg ggcgcggcta tccgccgctc cgggagttcc tggccggcta cctgctgcgc
      901 ttcgggctcg aggtgcgccc ccaggagatc ctgatcgtca acggctcgca gcagggcttc
      961 gatctggtcg cgcgcaccct gctggatccc ggcgacttcg tggcgatcga ggagccgtcc
     1021 tatccgcgcg ccatgcaggt cttccgcgcg ttcggggccc agctcctgcc ggtgccgatg
     1081 agggacggcg ggctcgatcc cgagcatctc gaccgcttgc tcgagcggca gtccccgaag
     1141 ctgctctact gccagcccag cgcccacaat cccaccgggc tcaccatgcg gcccgaggcg
     1201 cggaagcggc tcctggaagt ggcggcgcgg caccggctgc ccatcatgga ggacggcttc
     1261 gacgggagcc tgttcttcgg cccgcagccg ccggcgccgc tgaaggcgct cgacgtctcg
     1321 gggctcgtca tctacatcgg gacgttctcc aagatcctgt tccccgggct gcgcctcggc
     1381 tggatcgtgg ccgcgccgga gctgatcgac cggttggagg tcgcgaagga cctggcggat
     1441 atccacacga gcccgctcat ccaggccgcg gtctaccatt tctgccgcca gcgtctgctc
     1501 gaccgccacc aggcgcgagt cctgcgcgag tacggccggc gccgcgccgc gctgctcgcc
     1561 tcgctcgcca ggcgcatgcc ggccggcgtg acgtggaccg agaacgaggg gggcttttcg
     1621 ttgctggtga cgctgcccgc cggactggac gccgcgccgc tcctcgagcg cgccgcggcg
     1681 cgcggagtga tcttcacgcc cgggaacgcg ttcttcgtgg acggcggcgg cgagcggacg
     1741 ctgcggctgt ccttctcggc gctgccgctg gcccagatcg acgagggcgt caagcggctc
     1801 gccgagatca ttcgtgacgt gcagcgccag ccggagccgg tgggcctcgt ggccgccccg
     1861 gccgtgccgc tggtctagga ggtacctcgt ggatttccat accctggcgg cccgcggcgg
     1921 gcgcgacgtg ccctcggcca gccggcccct gacgcccccc atctaccaga ccaacgtgta
     1981 cgtgttcgag gacatggaca cggtcgagtc ggtgtgggag agcaagaagg cgggcttcgt
     2041 gtacggccgc tacggcaccg gcaaccacgc aatgctcgag gatctggtgg cggcgctcga
     2101 gggcgcggag acagcggtgg cgtgcgcgtc cggcatgggc gccaccaccg ccctgctgct
     2161 cgggctgttc gcgcccggcg atcacctggt ctccgcgcgc gatctctacg gctccaccgc
     2221 cgcgttcctg ggcgacgagg ggcggcggct cggggtcgag accgacttcg tggacgcaac
     2281 cgacatcggg gcgatcgtga aggcgctgcg gccgaccacg cgcgcggtct tcgtggaggc
     2341 g
//
LOCUS       DASUFA010000400         6326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1239894, whole genome shotgun sequence.
ACCESSION   DASUFA010000400 DASUFA010000000
VERSION     DASUFA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239894"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFX87_14490"
     CDS             <1..480
                     /locus_tag="VFX87_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996188.1"
                     /translation="DPASYHGEFYRFDSVRCLPHPVQKPHPPIWVGGHSKVALRRVAR
                     LGDGWHPVGANPAVPLRPPELRASLDELFRLTEAEKRDPSRLTISYKAPIYDPGQGVD
                     GGAERRPFSGSQQAIADDIGTFAGLGVSELIFDFRSESLAQSLERMERFVPILRQIA"
     gene            585..791
                     /locus_tag="VFX87_14495"
     CDS             585..791
                     /locus_tag="VFX87_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996189.1"
                     /translation="MPEQPPDVTLDEVKERARLAGLPIPANRLSLVRGFLSNALKPIR
                     ILDSRTVRTVEPAVTFDAKGGSGS"
     gene            788..2179
                     /locus_tag="VFX87_14500"
     CDS             788..2179
                     /locus_tag="VFX87_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4996190.1"
                     /translation="MSADELCYAGIRELAGLFRRRELSPVDYATDQLRRIETLDKSLN
                     AYVTVTAERALADARAAEAALKRGDAGPLTGIPIAYKDLYATKGIRTTAGSALLADWV
                     PDGDCTCVARLQHAGMVMLGKLITHEFAFGIQFPGHRFPPARNPWNLDHIPGGSSSGS
                     GAALAAGMTVGSLGSDTGGSIRGPAAFCGIAGLKPTYGRVSRAGVVTLSWTLDHTGPM
                     ARSAEDCAFMLQALAGHDPADPASAREPVADYLANLEGGVKGLRVGVVRDYFFEQVEP
                     ETVAAFERAAGELGRLGATVRDVTIPSIHAAPSFMVIMLTEAFAYHERDLRTRPELYG
                     DVLREKLMAGGLFRADEYVQAQRLRSQIREDTARALQSVDVLITPTALGPAPAFKVVL
                     DPDFPFAKSNMGPFNITGLPTLALPTGFSSTGLPLSFQISGRPFDEATVLRAGHAYQR
                     ATDWHRRRPPVRA"
     gene            2176..3057
                     /locus_tag="VFX87_14505"
     CDS             2176..3057
                     /locus_tag="VFX87_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4996191.1"
                     /translation="MTTVGTPGPVALDDLKGHVGRRETATDVVTAAPANLLRLTFGRD
                     EREFAPGDPLPPGWQSLYFLPRFPPAALRPDGSPLDTGVIPPMPLPRRMFAGERFRFH
                     RPLRIGETIRRETELADISVKTGGTGTLVFATVTQRIFTGETLAIEEERRTVFREEVR
                     AGERNQAPRRETAPDDTPWRRRIAPDPVLLFRFSALTFNSHRIHYDRPWAMEHEGYPG
                     LVVHGPLTSTLLIDFARDHNPGRVLTGYETQARAPLFDTAPFELRGRPAADGRGAELW
                     AVTPEGTVAMSARAELA"
     gene            3092..3481
                     /locus_tag="VFX87_14510"
     CDS             3092..3481
                     /locus_tag="VFX87_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996192.1"
                     /translation="MADELELRMDATALYREETYTDRRIGIIRVMTPVKTDGSIDLAR
                     KILYVGEAQLLSPVGALPITFEIDATSLGDAAEKFAEGAKVGVERAIRELQELRREQS
                     SNIVIPDRMPPDIGGRGGPRGPGRIQL"
     gene            3492..4307
                     /locus_tag="VFX87_14515"
     CDS             3492..4307
                     /locus_tag="VFX87_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4996193.1"
                     /translation="MNLLRPIDRYVTVQGLRFHVLDWGGEGRTPLLLLHGFTGHAHAW
                     DTLAIALQPHFHVYALDQRGHGDSDPAEVYGPSAAFDDLNGVIDHLGLAPLVVVGLSM
                     GGRNAMYFTSKRPEAVRKLVIVDIGPEISARAAQTPAGPPEPPFWDSIEQAAQHLFRG
                     NPYPGIHYYRWVAAQSLRQRPDGRIVWAWHPSVKTRRSTVEVDWWAVLRAIPVPTLVL
                     RGEESPILDRSVAERMAKELPQGRFVEIPRAVHTLHEDNPDAVLAALKPFLGF"
     gene            4324..6255
                     /locus_tag="VFX87_14520"
     CDS             4324..6255
                     /locus_tag="VFX87_14520"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX4996194.1"
                     /translation="MGVVRLEITRREPVLDGAAFKGAGGARSAYEKLEGVLHFSADPV
                     SRANEPIADLGLAPRNERGLVEYSADFYLLRPLDGGRHRLLLDVPNRGRKIALGMFNS
                     TPRSNDPTSRDDFGNGFLMRHGYTVAWIGWQPDVPRRDGMMALTVPRVSGVSERVRCE
                     FRPNARVDVLPLADRYHIPHPVARLDDPEALLSVREHADAPAVTVPRAAWRFPDPDSV
                     ALDGGFQPGRIYDCYYRAQDPPVVGLGFTAVRDTASFLRWGTAAEGNPCAGTIERSYV
                     FGVSQSGRFLRHLLYLGLDEDEAGRFVWDGVIPHVAGARRGEFNCRFGQPSLNAVHAV
                     GSLFPFTDDPQDDPVTGQRDALLRRLAGRAKPPRIFTINTSAEYWRGDGSLVHTDVGG
                     TRDVAPPPYVRQYLFAGSQHTPGALPPPASDPNTGGRGFHPFSVVDYSPLLRAALVNL
                     DRWVGEGVEPPPSAVPRLADGTAVAAETTRGVYAAIPGARFPDHVQRPQRLDFGPAVE
                     RGIATLPPKLGAPYVTFVSAVDRDGNERAGIRPPELRVPLATFMGWNPRHPEQGAPGD
                     IMAMMGSTLSFARTAAERARTGDPRLSIEERYAGREGYLARVRQDAEEMVAARHLLSE
                     DVDEVVERAGALWDFVCPR"
BASE COUNT          845 a         2369 c         2211 g          901 t
ORIGIN      
        1 gatccggcct cgtaccacgg cgagttctac cgcttcgact ccgtgcgctg cctgccccat
       61 cccgtgcaga agccgcaccc gccgatctgg gtgggcggcc acagcaaggt cgcgctgcgc
      121 cgggtcgcgc gtctgggcga cggctggcat ccggtggggg cgaatccggc cgtcccgctg
      181 cgaccgcccg agctgcgcgc gtcgctcgac gagctcttcc gcctcaccga ggcggagaag
      241 cgtgatccct cgaggctcac catctcctac aaggcgccga tctacgatcc ggggcagggg
      301 gtggacgggg gcgccgagcg gcggccgttc tcggggagcc agcaggccat cgccgacgat
      361 atcggcacct tcgccgggct cggggtgagc gagctgatct tcgacttccg tagcgagagc
      421 ctcgcgcaga gcctcgagcg catggagcgc ttcgttccga tcctccgcca gatcgcctga
      481 cgctctccct ctcccctttg gggagagggc agggtgaggg gggcggttgc tctcgcgcgc
      541 ggacccgtct actctagggc ccgtcgccga caaggagatc cgtcatgccc gagcagcccc
      601 ctgacgtcac gctggacgag gtaaaggagc gcgcccgtct cgccggcctg cccattcccg
      661 cgaaccgcct gtccctggtg cggggcttct tgagcaatgc cctcaagccg atccggatcc
      721 tcgacagccg gacggtgcgc accgtggagc cggcggtgac cttcgacgcc aagggcggga
      781 gcggctcgtg agcgccgacg agctctgcta cgcgggcatc cgggagctgg ccggcctctt
      841 ccgccgccgt gagctctcgc cggtggacta cgccaccgat cagctgcgcc gcatcgagac
      901 gctcgacaag agcctcaacg cctacgtgac ggtcaccgcc gagcgcgcgc tcgccgacgc
      961 ccgcgccgcg gaggccgcgc tcaagcgggg cgacgcgggg ccgctgaccg gcatcccgat
     1021 cgcctacaag gatctctacg cgaccaaggg gatccgcacc accgcgggct ccgcgctgct
     1081 ggccgactgg gtgcccgacg gagactgcac ctgcgtggcg cgcctgcagc acgccggcat
     1141 ggtcatgctc ggcaagctga tcacccacga gttcgccttc ggcatccagt tcccggggca
     1201 ccgctttccg cccgcccgca acccgtggaa cctcgatcac atcccgggcg gctcgagcag
     1261 cggctccggc gcggcgctgg ccgccggcat gacggtgggc tcgctcggct ccgacaccgg
     1321 cgggtccatc cgcggcccgg ccgccttctg cggcatcgcc gggctcaagc cgacctacgg
     1381 gcgcgtgagc cgcgcgggcg tggtcacgct ctcgtggacg ctcgatcaca ccggccccat
     1441 ggcgcgctcg gcggaggact gcgccttcat gctgcaggcg ctggccggcc acgacccggc
     1501 cgatccggcc tccgcccgcg agccggtcgc ggactacctg gccaacctcg agggcggcgt
     1561 caagggtctc cgggtcggcg tggtgcgcga ctacttcttc gagcaggtgg agccggagac
     1621 ggtcgccgct ttcgagcgcg cagcgggcga gctggggcgg ctcggcgcca ccgtgcggga
     1681 cgtgacgatc ccgagcatcc acgccgcgcc gtccttcatg gtgatcatgc tcaccgaggc
     1741 cttcgcctac cacgagcgcg atctgcgcac gcgcccggag ctctacggcg acgtgctgcg
     1801 cgagaagctg atggcgggcg ggctcttccg ggcggacgag tacgtgcagg cccagcgcct
     1861 gcgctcccag atccgcgagg acaccgcgcg cgcgttgcag tcggtggacg tgctgatcac
     1921 gcccaccgcg ctcggcccgg cgcccgcgtt caaggtcgtg ctcgatcccg acttcccgtt
     1981 cgccaagagc aacatgggcc cgttcaacat caccgggctg ccgaccctgg cgctgccgac
     2041 cgggttttcc tccaccggcc tgccgctgtc gttccaaatc tccggccggc ccttcgacga
     2101 ggcgaccgtg ctgcgcgcgg gccacgccta tcagcgcgcg acggactggc accgccgccg
     2161 cccgccggtc cgcgcatgac gaccgtaggg acgccgggcc ccgtcgcgct cgacgacctg
     2221 aaggggcacg tcggccggcg cgagactgcg accgacgtgg tcaccgccgc gcccgccaat
     2281 ctcctgcgtc tgaccttcgg tcgcgacgag cgcgaattcg cgccgggcga cccgctgccg
     2341 ccgggctggc agtcgctcta ctttctcccg cgctttccac cggcggcgct gcgccccgat
     2401 ggcagcccgc tcgacaccgg ggtgatcccg ccgatgccgc tgccccgccg catgttcgcg
     2461 ggcgagcggt tccgcttcca ccgcccgctg cgcatcggcg agacgattcg ccgggagacg
     2521 gagttggccg acatctcggt caagacgggc ggcaccggca ccctggtctt cgccacggtg
     2581 acccagcgca tcttcaccgg tgagacgctg gccatcgagg aggagcgccg caccgtgttc
     2641 cgcgaggagg tccgggcggg cgagcgcaac caggcgcccc gccgcgagac cgcgcccgac
     2701 gacaccccgt ggcggcggcg gatcgcgccg gacccggtgc tgctcttccg cttctccgcg
     2761 ctgacgttca acagccaccg catccactat gaccggccct gggccatgga gcacgagggc
     2821 tatccgggcc ttgtcgtgca cggcccgctc acctccacgc tcctgatcga cttcgcgcgc
     2881 gaccacaatc cgggccgcgt gctcacgggc tacgagaccc aggcccgcgc cccgctcttc
     2941 gataccgcgc ccttcgagct gcgcggccga cccgccgcgg acggccgcgg cgcggagctg
     3001 tgggcggtca cccccgaggg tacggtggcg atgagcgcgc gggccgagct cgcgtgacgg
     3061 actattcgat tgaaacggag gattacccac gatggccgat gagctcgagc tgcgcatgga
     3121 tgcgactgcc ctctatcgcg aggagaccta caccgaccgc cgcatcggca tcatccgcgt
     3181 gatgacgccg gtgaagacgg acggttcgat cgaccttgcc cgcaagatcc tctacgtggg
     3241 cgaggcccag ctcctctccc cggtgggcgc cctgccgatc accttcgaga tcgacgcgac
     3301 ttcgctcggc gatgcggccg agaagttcgc ggagggcgcc aaggtgggag tggagcgcgc
     3361 gatccgcgag ctgcaggagc tgcgccggga gcagtcctcc aacatcgtca tccccgaccg
     3421 catgccgccc gacatcggcg gccgcggcgg accgcggggc ccgggccgca tccagctctg
     3481 atcgcgcccg tatgaacctg ctgcggccga tcgaccggta cgtcaccgtc caggggctgc
     3541 gcttccacgt gctcgactgg ggcggggaag gccgcacccc gctgctcctc ttgcacggct
     3601 tcaccgggca cgcgcatgcc tgggacacgc tcgccatcgc gctccagccg cacttccacg
     3661 tctatgcgct cgatcagcgc ggccacggcg acagcgatcc ggcggaggtc tacgggcctt
     3721 cggccgcgtt cgacgatctg aatggggtga tcgaccatct cgggctggcc ccgctcgtcg
     3781 tcgtgggcct gtccatgggc gggcggaacg cgatgtactt cacctccaag cgtcccgagg
     3841 cggtgcggaa gctcgtgatc gtggacatcg ggcccgagat cagcgcgcgg gcggcccaga
     3901 ccccggcggg cccgcccgag cctccattct gggacagcat cgagcaggcg gcccagcatc
     3961 tcttccgcgg caacccgtat cccggcatcc actactaccg ctgggtggcg gcccagagcc
     4021 tgcgccagcg gccggacggg cgcatcgtat gggcctggca cccgagcgtg aagacgcggc
     4081 gctcgacggt ggaggtggac tggtgggcgg tgctgcgggc gatcccggtg ccgaccctgg
     4141 tgctgcgggg cgaggagagc ccgatcctcg accgctccgt ggcggagcgc atggccaagg
     4201 agctgccgca gggccggttc gtcgagatcc cccgcgcggt gcacacgctg cacgaggaca
     4261 atccggacgc cgtcctcgcc gcgctcaagc cgttcctcgg cttctaggtc ggaggctcac
     4321 gcgatgggag tcgtcaggct ggagatcacc cggcgcgagc cggtgctgga cggcgcggcg
     4381 ttcaagggcg ccggcggggc gaggagcgcc tacgagaagc tcgagggcgt gctgcacttc
     4441 tcggcggatc cggtgtcccg ggcgaacgag ccgatcgcgg acctggggct ggctccccgc
     4501 aacgagcgcg ggctcgtgga gtactcggcc gacttctatc tcctgcggcc gctcgacggc
     4561 gggcgccacc ggctgctcct cgacgtgccg aaccgcgggc gcaagatcgc gctgggcatg
     4621 ttcaacagca ccccgcgctc gaacgacccg acctcgcggg acgatttcgg caacggcttc
     4681 ctgatgcggc acggctacac ggtggcctgg atcggctggc agcccgacgt gccgcgccgc
     4741 gacggcatga tggcgctgac cgtgccccgg gtgtccggcg tctccgagcg ggtgcgctgc
     4801 gagttccggc cgaacgcgcg ggtggacgtg ctgccgctcg ccgaccgcta tcacatcccg
     4861 catccggtgg cccgcctcga cgaccccgag gccctcctct ccgtgcgcga gcacgccgac
     4921 gcgcccgcgg tcacagtgcc gcgcgccgcc tggcgcttcc ccgaccccga ctcggtcgcg
     4981 ctcgacggcg gcttccagcc gggccgtatc tacgactgct actaccgagc gcaagacccg
     5041 ccggtggtcg gcctcggttt caccgcggtg cgggataccg cgagcttcct gcgctggggc
     5101 accgccgccg agggcaatcc ctgcgccggg accatcgagc ggtcgtacgt gttcggcgtc
     5161 tcgcagagcg gccgcttcct gcggcacctg ctctacctgg gcctcgacga ggacgaggcc
     5221 ggccgcttcg tctgggacgg ggtgatcccg cacgtggccg gggcccggcg cggcgagttc
     5281 aactgccgct tcggccagcc ctcgctcaac gcggtgcacg cggtcggcag cctgttcccg
     5341 ttcaccgacg acccacagga cgatccggtg accggccagc gcgacgcgct gctccggcgc
     5401 ctggcggggc gcgcgaagcc gccacgcatc ttcacgatca acacctcggc cgagtactgg
     5461 cgcggcgacg gctcgctcgt gcacaccgac gtgggcggca cgcgcgatgt ggccccgcca
     5521 ccctacgtgc gccagtacct cttcgcgggg agccagcaca cgcccggcgc gctgccgccg
     5581 cccgcgtcgg atcccaatac cgggggccgg ggcttccacc cgttcagcgt ggtggactac
     5641 tcgccgctcc tgcgcgccgc gctggtcaat ctcgatcggt gggtcggcga gggggtggag
     5701 ccgccgccca gcgcggttcc gcgcctggcc gacggcaccg cggtggccgc cgagacgacg
     5761 cgcggcgtct acgcggcgat tccgggcgcg cgcttccccg accacgtcca gcgaccgcag
     5821 cggctcgact tcggtcccgc cgtggagcgc gggatcgcca cgctgccgcc caagctcggc
     5881 gcgccctacg tgacgttcgt ctccgcggtg gaccgcgacg gcaacgagcg cgcgggcatc
     5941 cggccgcccg agctacgggt gccgctggcc accttcatgg gctggaaccc gaggcatccg
     6001 gagcagggcg ccccgggtga catcatggcc atgatgggct cgacgctctc cttcgcgcgc
     6061 accgcggccg agcgggcgcg gacgggtgac ccgcggctct ccatcgagga gcgatatgcc
     6121 ggccgcgagg gctatctcgc gcgggtgcgc caggacgcag aggagatggt cgcggcgcgg
     6181 cacctcctca gcgaggatgt ggacgaggta gtggagcgcg cgggcgcgct ctgggacttc
     6241 gtctgcccgc gctgagcctg ccctggccac gccgctgatc gagtgccacg cccacgtcct
     6301 gcccgacggg gcgctggccc gcttca
//
LOCUS       DASUFA010000401         9907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_101205, whole genome shotgun sequence.
ACCESSION   DASUFA010000401 DASUFA010000000
VERSION     DASUFA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101205"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1153
                     /locus_tag="VFX87_14525"
     CDS             38..1153
                     /locus_tag="VFX87_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996195.1"
                     /translation="MLKRFALLVSLFVLAVVPAAAQQGPIKIGAILPLTGSGASYGVW
                     MKGGTEMAAEEINAAGGIGGRKLEVIYEDHAADASKAVNAMRRLVEVEKIPFTLTSYS
                     APTLAIQPIGSQNKVVMMNGGGQSDNLANKDYLYNNIPVVSNEVGVLCDWLTKEKRFK
                     AAVMIVANDEAGRNSAKSFRERFGAGGGKVLAEEQIALDGNDFRAQLAKLKAQGGDLL
                     FISSYGRNVAIVADQARELGITLPMAATSWVQIPEVMKSKGAQGLLTTRLPFDPESAF
                     ARKFKERYKTDAGFFAVQYYSGTRIFAAAAEAAMKKNAGKLDGEGVKTAIETMKAFDT
                     ISGKLVFQPNHAAVMDIEVGVLKDGKVEVEKVIKAAH"
     gene            1173..2057
                     /locus_tag="VFX87_14530"
     CDS             1173..2057
                     /locus_tag="VFX87_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996196.1"
                     /translation="MDLVLQLLATGLVVGSLYALCAIGWGLIYGTTLHFHVAHGAVFA
                     LAAYYAYVGQKLLHLPLVAAVLLAIVASAASGLLIDLLLYQQLERRGAFRTSIFIASL
                     GLLILLENVLAIVFTPDPMRMDIGVLHTAVIIGPVFLTYLHILTVALTIVAYLALMLF
                     LKRSRWGQAIRAVSSAPEMARTVGIDLHRVHLLTYAIGSALAAPAGILVAMDVGAEPF
                     RGTRFVLLASVGVIMGGIGSIPGAMLGGVFLGLLENLGIWKLPSEWQSAISFGVFLVF
                     IVLRPRGFFGRTIHSAEI"
     gene            2065..2946
                     /locus_tag="VFX87_14535"
     CDS             2065..2946
                     /locus_tag="VFX87_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996197.1"
                     /translation="MAAYIAHYLVMVGIYTILATSLNLLVGYSGIFSLAHAAIYGIGA
                     YASALAALKLGLGFWGGLLVAAVVGALAAALVAIPSLRVAGDYYIVASFGLQVVILTV
                     FINWTSLTNGHAGLPGIPRPSVFGLVIDNPFKYVVLSLALAALTYAICHRLTGSAFGR
                     MLRAVREDEIAAQATGKNVVAVKITITTISSALGALAGSLYAHYITYINPTSFTLDES
                     IFITSLVILGGTERLAGPFVGALILLAVPEALKFLAIPDTVAAPMRQILYGALLILFM
                     FVRPEGILGRARTRPAA"
     gene            2960..3721
                     /locus_tag="VFX87_14540"
     CDS             2960..3721
                     /locus_tag="VFX87_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996198.1"
                     /translation="MAAPILTVQGVSKAFGGIRAVEDCSFDVAPRLITGLIGPNGAGK
                     TTVFNLITGFLRADGGSIRYKGEELLGRSPHEVETRGVVRTFQHLRLWGKMTVLENVL
                     LGCRTPLGENVVSLFLRPRRVREEEAAARRRALEVLEFFGLAGRAHELAEDLAYPEQK
                     LLSMARIFATDAEVLLLDEPTSGLDTESLGRIVPMVRRLIEHGKTVLLIEHNMELIAQ
                     LSDEVVFLHQGRVMARGRAEQITRDPALTEIYFGV"
     gene            3726..4433
                     /locus_tag="VFX87_14545"
     CDS             3726..4433
                     /locus_tag="VFX87_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996199.1"
                     /translation="MALLEVEGLEAGYGKKTVLQGVSFRVDEGEVVALLGHNGAGKST
                     TLKTILGLLPARAGQVRFGGEPWANGDPAENVRRGLALVPQGRGVFPDLTVVENLALG
                     AYTQSDRAGMTTRMNEILDLFPILAERRTQQVGTMSGGQQQMVAVGTALMQRPRLMMM
                     DEPSIGLAPVLVQRVLETAVQINRRFGTAIVLVEQNIKTALGMARRAYVMKSGRIVLE
                     KPAAELLAAQDSWWELY"
     gene            4433..5959
                     /locus_tag="VFX87_14550"
     CDS             4433..5959
                     /locus_tag="VFX87_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4996200.1"
                     /translation="MDELEPTSRGLLAWAAARYRDAEALAHGDRRWSFEGLLDQASRL
                     AAGLRARGIARGDRVGLWLPNWPEWVFARHALGILGAVTVPINTRYKADELAYCLRQG
                     ECRALLSADRFLGLDFRATVEGIRAALPALEWVAYPDELAVMLAGPPLPEARWPALSP
                     DDVGYMIYTSGTTSFSKGVQLTHRNLSRNTIEAGRAVDLRAGERMLIAVPLFSSFGTC
                     HLSFAALSAGACLVLQDHFDPPATLALLERERITYLPCVDTMLLAMAETGRVGDHDLR
                     HLTRIMAAPLNPAGITAAYERFKAAQVWTGYGLTEASAISGVHLARGPEDEALFRPLP
                     GIGVRVVDPETSAPRAVGGEGEIQIRGGHVTPGYYKMPEQTAALFTADGWMRTGDLGR
                     EESPGLFRFLSRLKEVLKTGGFLVAPLEVEAVLGGHPAVAEACVVPRPDGRLGEVGVA
                     FVQLRPGAACAAKELLDLCAARLANFKVPRQIVFVDDYPRTATGKIQRARLKERVGGA
                     "
     gene            5965..7107
                     /locus_tag="VFX87_14555"
     CDS             5965..7107
                     /locus_tag="VFX87_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996201.1"
                     /translation="MDFELTPEQRELKQAVRRLAEREFRPRAARWDEEEAFPWENIAA
                     LRDAGLLALALPEAYGGAGGTVFDLALVMEELARCCVATAFIVGQQNGFGAKAIALYG
                     TAAQRARHLPAIGRGERLIAWAMSEPGAGSDLRNLACRARRDGDAYVIDGRKTFCSLG
                     GVSHLNVVICRFDGVAGFPGLGALLVEADAPGFSRGRDIPEMGLRGTAMADLVFEDCR
                     VPAGNLLIAPGEFRKILQIMDGERTAGNPPMSLGIAQGALEEALRFAEQRQAFGQRVA
                     DFQGIRWMLADMAVKVEAARLLVYRAAWRAARGAPDPFEASVAKAYTNEIAVSVCNDA
                     LQIHGGYGYTREFPVERMLRDARGLCIGDGTTQIQKNIIAKHLVKP"
     gene            7104..8264
                     /locus_tag="VFX87_14560"
     CDS             7104..8264
                     /locus_tag="VFX87_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX4996202.1"
                     /translation="MMRDVCIVGMAETAVGRLEGSTSLGLQAEASRLALADAGLRSVE
                     VDAVYNLDPYTQAMSMFAQSLLEYMGLAPTLAATIDAGGTVTPMHMVLNAIAAIGQGY
                     AEVALCTFGENALTGRPKGVHGLPQGPSLGQEEFEEPFGCIGMVIPYALAAQRHMHHY
                     GTTKQQLGAVAVSARRHAALNPRAQMRKPLTMADYLAARPIADPFGLLDCSVMSDGAG
                     AVVLSTVERARDLATTPVRVLGFGGKATHKNVNQMPDLDALGLRPAGEMALGRAGIGI
                     GDADFCCIHDAFTISTIVGVEALGLCADGEGGAYAAAGRLDLGGPCPVNPHGGLLSHA
                     HIGGMNHLCEAVRQLRHEAEPERQVPDARVGVVSGNGGVFSLCGVMVLARGI"
     gene            8272..8691
                     /locus_tag="VFX87_14565"
     CDS             8272..8691
                     /locus_tag="VFX87_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX4996203.1"
                     /translation="MATPQYGKPLPRPTAATAPFWEGCRAGVLRLPWCRACGRPHFFP
                     RSLCPHCLGPELDWRDATGRGRVWSHSTVRLSFWGKAFDDSLPYVVAIVELEEGVRLL
                     SNIVGCPPDAVAIGMPVRVTFDAVTPEITLPRFTVSG"
     gene            8688..9818
                     /locus_tag="VFX87_14570"
     CDS             8688..9818
                     /locus_tag="VFX87_14570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4996204.1"
                     /translation="MSPDLPRRADIVIIGAGAIGASIAYQLGRRGVRDVVVLERDAVG
                     AGSTSKAAGGIRVQFATRVEIELSLRGLAFFKRFEDEMGVPCDFHQEGYLFVVTDEPT
                     LARFRDNVALQRSMGADVRVIAPDDARALVPSLNMDDALAAVWGPMDGHASPNDVVQA
                     YAAQARARGVRIVEDTPVTGIVVERGRVAGVLTPAGAIATPLVVNAAGPWAPLVGRMA
                     GLDLPVDPRRRHIFVTDAFDGIRHPLPLVTDTASGFYCRSEQGAILMSPGDIGAVTEY
                     EARVDWSVLEVAVEKAIRRIPALEGAQVRHAWAGLRPLTPDGRAILDWAPGVEGLYLA
                     VGFCGHGFQHSPGVGETVAEVLLDGRSTLDIQDLRLGRFPAA"
BASE COUNT         1331 a         3552 c         3536 g         1488 t
ORIGIN      
        1 cgactacgcc cgcacccctg accaggaggc cccttcaatg ctgaagcgat tcgctctgct
       61 cgtctcgctg ttcgtgctcg ccgtcgtgcc cgccgcggcc cagcagggcc cgatcaagat
      121 cggcgcgatc ctgccgctga ccggctccgg cgcctcctac ggggtgtgga tgaagggtgg
      181 caccgagatg gcggccgagg agatcaacgc ggccggcggg atagggggcc gcaagctgga
      241 ggtcatctac gaggaccacg cggcggacgc ctccaaggcg gtcaacgcga tgcggcggct
      301 cgtcgaggtg gagaagatcc cgttcacgct cacgtcctac tcggcgccga ccctggccat
      361 ccagccgatc ggctcccaga acaaggtcgt gatgatgaac gggggcgggc agtccgacaa
      421 cctcgccaac aaggactacc tctacaacaa tatcccggtg gtctcgaacg aggtcggcgt
      481 gctgtgcgac tggctgacca aggagaagcg gttcaaggcc gcggtcatga tcgtggccaa
      541 cgacgaggcg ggccgcaact cggccaagtc cttccgcgag cgcttcgggg cggggggcgg
      601 gaaggtcctc gccgaggagc agatcgcgct cgacggcaac gacttccgcg cgcagctcgc
      661 caagctcaag gcgcagggcg gggacctgct cttcatctcc tcctacggcc gcaacgtggc
      721 gatcgtggcc gaccaggcgc gcgagctcgg catcacgctg ccgatggccg cgacctcgtg
      781 ggtgcagatc cccgaggtga tgaagtcgaa gggcgcccag gggctcctga cgaccagact
      841 gccgttcgat ccggagtcgg ccttcgcccg gaagttcaag gagcgctaca agaccgacgc
      901 cggcttcttc gcggtgcagt actacagcgg gacccggatc ttcgcggcgg ccgccgaggc
      961 ggccatgaag aagaatgcgg gcaagctgga cggcgagggg gtcaagaccg cgatcgagac
     1021 catgaaggcc ttcgacacca tctcgggcaa gctggtcttc cagcccaacc acgccgcggt
     1081 catggacatc gaggtgggcg tgctcaagga cggcaaggtc gaggtcgaga aggtcatcaa
     1141 ggccgcgcac tagccgcgcg ggcgcagccg ctgtggatct cgtcctccag ctcctcgcca
     1201 cgggcctcgt ggtggggagc ctgtacgccc tctgcgccat cggctggggc ctgatctacg
     1261 gcaccacgct gcacttccac gtggcccacg gggcggtgtt cgcgctcgcc gcgtactacg
     1321 cctacgtggg ccagaagctg ctccacctgc cgctggtggc ggcggtcctg ttggccatcg
     1381 tggcctcggc ggcctcgggc ctcctgatcg atctgctcct ctaccagcag ctcgagcggc
     1441 gcggcgcctt ccgcaccagc atcttcatcg cctcgctcgg cctgctcatc ctgctggaga
     1501 acgtgctcgc catcgtgttc accccggatc cgatgcgcat ggacatcggg gtgctccaca
     1561 ccgcggtgat catcgggccg gtcttcctca cgtatctcca catcctcacc gtcgcgctga
     1621 ccatcgtggc ctacctggcc ttgatgctct tcctcaagcg ctcgcgctgg gggcaggcga
     1681 tccgcgcggt gtcgagcgct cccgagatgg cccgcacggt gggcatcgat cttcaccgcg
     1741 tgcatctgct gacctacgcg atcggatcgg ccctggccgc ccccgcaggt atcctggtgg
     1801 cgatggacgt gggcgccgag cccttccggg gcacgcgctt cgtgctgctg gcctcggtgg
     1861 gcgtcatcat gggcggcatc ggctccatcc cgggcgcgat gctgggcggc gtcttcctgg
     1921 gcctgctcga gaacctgggg atctggaagc tgccgtcgga gtggcagtcg gcgatcagct
     1981 tcggggtctt cctggtcttc atcgtgctgc ggccgcgcgg cttcttcggc cgcaccatcc
     2041 actccgccga gatctgaccc cgccatggcc gcctacatcg cgcactacct ggtcatggtc
     2101 gggatctaca caatcctcgc cacctcgctc aacctgctgg tgggctactc cgggatcttc
     2161 tcgctcgccc acgccgcgat ctacggcatc ggcgcctacg cctcggccct cgccgcgctc
     2221 aagctgggcc tcgggttctg gggcgggctg ctggtggccg cggtggtggg cgcgctcgcg
     2281 gcggccctgg tggcgatccc gtcgctgcgg gtggcgggcg actactacat cgtcgcgtcc
     2341 ttcgggctgc aggtggtgat cctcaccgtc ttcatcaact ggacctcgct caccaacggc
     2401 cacgcgggcc tgcccggcat cccgcgcccg agcgtgttcg ggctcgtgat cgacaacccg
     2461 ttcaagtacg tggtgctctc gctcgcgctc gccgcgctca cctacgcgat ctgccaccgg
     2521 ctcaccggct ccgcgttcgg gcgcatgctg cgcgcggtgc gcgaggacga gatcgcggcc
     2581 caggccaccg gcaagaacgt ggtggcggtg aagatcacca tcaccaccat ctcctccgcg
     2641 ctgggcgccc tcgccgggag cctctacgct cactacatca cctacatcaa ccccaccagc
     2701 ttcacgctgg acgagtccat cttcatcacc tcgctcgtga tcctgggcgg gaccgagcgg
     2761 ctggccgggc cgttcgtggg agccctgatc ctcctcgcgg tcccggaggc gctcaagttc
     2821 ctcgcgatcc cggacacggt ggcggcgccg atgcgccaga tcctctacgg agcgctcctc
     2881 atcctgttca tgttcgtgcg gccggagggc atcctcgggc gcgcccggac ccgtccggcg
     2941 gcctgagacg gccagcgcga tggcggctcc catcctgacg gtgcagggcg tgtccaaggc
     3001 cttcggcggc atccgggcgg tggaggactg ctcgttcgac gtggccccgc gcctgatcac
     3061 cgggctgatc ggccccaacg gcgccggcaa gaccaccgtg ttcaacctga tcaccggctt
     3121 tctgcgagcg gacggcggct ccatccgcta caagggcgag gagctgctcg ggcggtcccc
     3181 gcacgaggtc gagacccgcg gggtggtgcg caccttccag cacctccgcc tctggggcaa
     3241 gatgacggtg ctggagaacg tgctgctggg ctgccgcacg cccctcgggg agaacgtggt
     3301 ctcgctcttc ctgcggccgc ggcgcgtgcg ggaggaggaa gcggcggcgc ggcgccgcgc
     3361 gctcgaggtg ctggagttct tcgggctggc cggccgggcc cacgagctgg ccgaggacct
     3421 ggcctacccg gagcagaagc tcctctccat ggcgcgcatc ttcgccaccg acgcggaggt
     3481 gctgctcctc gacgagccga cctccgggct cgacaccgag tcgctcggcc gcatcgtgcc
     3541 catggtccgg cggctcatcg agcacggcaa gacggtgctg ctgatcgagc acaacatgga
     3601 gctgatcgcc cagctctccg acgaggtggt cttcctccac cagggccgcg tgatggcgcg
     3661 cggccgcgcc gagcagatca cgcgcgaccc cgcgctcacc gagatctact tcggggtcta
     3721 gcccgatggc gctcctggag gtcgaggggc tcgaggccgg ctacggcaag aagaccgtcc
     3781 tgcagggcgt gtccttccgg gtggacgagg gcgaggtggt ggcgctgctg ggccacaacg
     3841 gcgccggcaa gtccaccacg ctcaagacca tcctgggcct cctgcccgcg cgggcgggcc
     3901 aggtgcgctt cggcggcgag ccgtgggcca acggggatcc ggcggagaac gtgcgccgcg
     3961 ggctggccct cgtgccgcag ggccggggcg tcttccccga tctgacggtg gtggagaacc
     4021 tggcgctggg cgcctatacc cagtcggacc gcgccgggat gacgacgcgc atgaacgaga
     4081 tcctcgacct cttcccgatc ctggccgaac ggcgcacgca gcaggtgggg acgatgtcgg
     4141 gcggccagca gcagatggtg gcggtcggca ccgcgctcat gcagcggccg cgcctcatga
     4201 tgatggacga gccgtccatc ggcctggccc cggtgctggt gcagcgcgtg ctcgagaccg
     4261 cggtccagat caaccggcgc ttcggcaccg ccatcgtgct ggtggagcag aacatcaaga
     4321 ccgcgctcgg catggcccgg cgcgcctacg tgatgaagtc gggccgcatc gtgctggaga
     4381 agccggccgc cgagctgctg gccgcgcagg actcgtggtg ggagctctac tgatggacga
     4441 gctcgagccg acctcgcgcg ggctgctcgc gtgggcggcc gcgcgctatc gcgacgcgga
     4501 ggcgctcgcc cacggggacc ggcggtggag cttcgagggg ctgctggacc aggcgagccg
     4561 gctcgcggcg gggctgcgcg cccgcgggat cgcgcggggc gatcgcgtgg gcctgtggct
     4621 gcccaactgg cccgagtggg tcttcgcccg gcacgcgctc ggcatcctgg gcgcggtgac
     4681 ggtgcccatc aacacgcgct acaaggccga cgagctcgcc tactgcctcc ggcagggcga
     4741 gtgccgggcg ctgctctcgg ccgaccggtt cctgggcctc gactttcgcg cgacggtcga
     4801 gggaatccgc gcggccctgc ccgcgctcga gtgggtggcc tatcccgacg agctcgccgt
     4861 gatgctcgcc ggcccgccgc tccccgaggc gcgctggccc gccctgtcgc ccgacgacgt
     4921 gggctacatg atctacacct cgggcacgac gtcgttctcc aagggcgtcc agctcaccca
     4981 ccgcaacctc tcgcgcaaca cgatcgaggc cggccgcgcg gtggacctgc gggcgggcga
     5041 gcgcatgctg atcgcggtgc cgctcttctc ctcgttcggc acctgccatc tctcgttcgc
     5101 cgcgctctcc gccggggcgt gcctcgtgct gcaggaccac ttcgatcccc ccgcgacgct
     5161 cgcgctgctc gagcgcgagc gcatcaccta cctgccgtgc gtggacacca tgctgctggc
     5221 catggcggag acggggcggg tcggcgacca cgatctccgg cacctcaccc gcatcatggc
     5281 cgcgccgctc aacccggccg gcatcaccgc ggcctacgag cgcttcaagg ccgcccaggt
     5341 ctggacgggc tacgggctca ccgaggccag cgcgatcagc ggcgtccacc tggcccgcgg
     5401 cccggaggac gaggcgctct tccgcccgct gcccggcatc ggagtccggg tggtggatcc
     5461 ggagacgagc gcgccgcgag cggtgggcgg cgaaggcgag atccagatcc ggggcgggca
     5521 cgtgacgccc ggctactaca agatgccgga gcagaccgcc gcgctcttca ccgccgacgg
     5581 ctggatgcgc acgggcgatc tgggccgcga ggagtcgccc ggcctcttcc gcttcctgag
     5641 ccggctcaag gaagtgctca agaccggcgg tttcctggtc gccccgctcg aggtcgaggc
     5701 cgtgctgggt ggccaccccg cggtggcgga ggcctgcgtg gtgccgcggc ccgacggcag
     5761 gctgggcgag gtcggcgtgg ccttcgtcca gctccggccc ggcgccgcct gcgcggcgaa
     5821 ggagctgctc gacctgtgcg cggcgcgtct ggccaacttc aaggtcccgc gccagatcgt
     5881 cttcgtggac gactatccgc gcaccgccac cgggaagatc cagcgggcgc gcctcaagga
     5941 gcgcgtgggc ggggcctgag cggaatggac ttcgagctga cccccgagca gcgcgagctc
     6001 aagcaggcgg tccgtcgcct cgccgagcgc gagttccgcc ccagggccgc gcgctgggac
     6061 gaggaggaag ccttcccgtg ggagaacatc gcggccctgc gcgacgcggg cctcctggct
     6121 ctggcgctgc ccgaggccta cggcggcgcg ggcggcacgg tcttcgatct cgcgctcgtg
     6181 atggaggagc tggcgcgctg ctgcgtggcc accgcgttca tcgtgggcca gcagaacggc
     6241 ttcggggcca aggccatcgc gctctacggc accgcggcgc agcgggcgcg gcatctgccc
     6301 gcgatcggtc gcggcgagcg gctcatcgcg tgggccatga gcgagcccgg ggcggggtcg
     6361 gacctgcgca acctggcctg ccgcgcgcgc cgtgacggcg acgcgtacgt gatcgacggc
     6421 cgcaagacct tctgctcgct gggcggggtg tcgcatctca acgtggtgat ctgccgcttc
     6481 gacggggtgg cgggcttccc gggcctgggc gcgctgctgg tcgaggccga cgcgcccggc
     6541 ttctcgcgcg gccgcgacat cccggagatg gggctgcgcg gcaccgccat ggccgacctg
     6601 gtcttcgagg actgccgcgt gcccgccggc aatctgctga tcgcgcccgg cgagttccgg
     6661 aagatcctgc agatcatgga cggcgagcgc accgcgggca atccgcccat gtcgctcggg
     6721 atcgcccagg gcgcgctcga ggaagcgctc cgcttcgcgg agcagcggca ggccttcggg
     6781 cagcgggtgg ccgacttcca gggcatccgc tggatgctgg ccgacatggc ggtgaaggtg
     6841 gaagcggcgc ggctgctcgt ctaccgcgcg gcgtggcggg ccgcccgggg cgcgccggac
     6901 cccttcgagg cctcggtggc caaggcgtac accaacgaga tcgcggtctc ggtgtgcaac
     6961 gacgcgctcc agatccacgg cgggtacggc tacacgcgcg agttcccggt ggagcgcatg
     7021 ctgcgcgacg cccgcggcct ctgcatcggc gacggcacca cgcagatcca gaagaacatc
     7081 atcgccaagc acctggtgaa gccgtgatgc gcgacgtctg catcgtgggc atggccgaga
     7141 ccgcggtggg ccgcctcgag ggcagcacgt ccctcgggct ccaggccgag gcctcgcgcc
     7201 tggccctggc cgacgcgggc ctcaggtccg tcgaggtgga cgcggtgtac aacctcgatc
     7261 cctacacgca ggccatgagc atgttcgccc agagcctgct cgagtacatg gggctcgccc
     7321 cgacgctggc cgccaccatc gacgcgggcg gcacggtcac gccgatgcac atggtcctga
     7381 acgcgatcgc ggccatcggg cagggctacg cggaggtggc gctctgcacc ttcggcgaga
     7441 acgcgctcac cggccgaccc aagggggtgc acgggctgcc gcaggggccc tcgctcggcc
     7501 aggaggagtt cgaggagccc ttcggctgca tcggcatggt gatcccgtac gcgctggccg
     7561 cccagcggca catgcaccac tacggcacca ccaagcagca gctcggcgcg gtcgcggtct
     7621 ccgcccgccg gcacgccgcg ctcaacccgc gcgcccagat gcggaagccc ctgaccatgg
     7681 ccgactacct ggccgcccgc cccatcgccg atccgttcgg cctgctcgac tgctcggtga
     7741 tgtcggacgg cgcgggcgcg gtggtgctca gcaccgtgga gcgcgcgcgc gatctcgcca
     7801 cgacgccggt gcgcgtgctc gggttcgggg gcaaggcgac ccacaagaac gtgaaccaga
     7861 tgcccgacct cgacgcgctc ggcctgcgcc cggccggcga gatggcgctc ggccgcgccg
     7921 gcatcgggat cggcgacgcg gacttctgct gcatccacga tgcgttcacg atctcgacca
     7981 tcgtgggcgt cgaggccctc ggcctctgcg ccgacggcga gggcggcgcg tacgcggcgg
     8041 cggggcggct cgacctgggc ggcccctgcc cggtgaatcc ccacggcggg ctcctctcgc
     8101 acgcccacat cggcgggatg aatcacctct gcgaggcggt ccgccagctc cgccacgagg
     8161 ccgagcccga gcgccaggtg cccgacgcgc gcgtgggcgt ggtcagcggc aacggcgggg
     8221 tgttctcgct ctgcggcgtg atggtgctcg cccgtgggat ctgaggcggc ggtggcgacg
     8281 ccccaatacg gcaagccgct gccgcgcccg accgcggcca ccgcgccgtt ctgggagggc
     8341 tgccgcgcgg gcgtgctgcg gctgccctgg tgccgcgcct gcggccggcc ccacttcttt
     8401 ccgcgctcgc tctgcccgca ctgcctcggc cccgagctcg actggcgcga cgccacgggg
     8461 cgcgggcgcg tgtggagcca ctccaccgtg cgcctgtcgt tctggggtaa ggccttcgac
     8521 gacagcctgc cgtacgtggt ggccatcgtg gagctggagg agggcgtgcg cctgctgagc
     8581 aatatcgtcg gctgcccgcc ggacgccgtc gcgatcggga tgccggtgcg ggtgaccttc
     8641 gacgcggtca ccccggagat cacgctgccg cggttcacgg tgtccggatg agccccgatc
     8701 tcccgcgccg cgccgacatc gtgatcatcg gagccggcgc catcggcgcg agcatcgcct
     8761 accagctcgg ccggcgcggc gtccgcgacg tggtggtgct cgagcgcgac gcggtcggcg
     8821 ccggctcgac cagcaaggcc gcgggcggca tccgcgtgca gttcgcgacc cgggtggaga
     8881 tcgagctctc gctccgcggc ctcgccttct tcaagcgctt cgaggacgag atgggcgtgc
     8941 cctgcgattt ccaccaggag ggctacctct tcgtggtcac cgacgagccg acgctggccc
     9001 gctttcgcga caacgtggcc ctccagcgga gcatgggcgc ggacgtgcgg gtgatcgccc
     9061 ccgacgacgc gcgcgccctg gtgccgtccc tcaacatgga cgacgccctc gccgcggtct
     9121 gggggcctat ggacggccac gcctcgccca acgacgtggt gcaggcctac gcggcccagg
     9181 ctcgggctcg cggcgtccgc atcgtggagg acacgccggt gaccgggatc gtggtggagc
     9241 gcggccgggt ggcgggcgtc ctcaccccgg ccggcgccat cgcgacgccg ctcgtggtga
     9301 acgcggccgg cccctgggcg ccgctggtcg gccggatggc cgggctcgac ctgccggtgg
     9361 acccgcgacg gcgccacatc ttcgtgaccg acgccttcga cggcatccgc catccgctgc
     9421 ccctcgtcac cgataccgcc agcggcttct actgccggag cgagcagggg gccatcctga
     9481 tgagcccggg cgatatcgga gccgtcaccg agtacgaggc gcgggtggac tggagcgtgc
     9541 tggaggtggc ggtcgagaag gcgatccggc ggatcccggc gctcgagggc gcccaggtac
     9601 gccacgcgtg ggccgggctg cggccgctca cgccggacgg ccgcgccatc ctcgactggg
     9661 ccccgggtgt ggaggggctc tacctggcgg tcggtttctg cggacacggc ttccagcatt
     9721 cccccggggt gggcgagacg gtggccgagg tgttgctcga cggtcgctcg acgctcgaca
     9781 tccaggacct gcgtctcggc cgctttcccg ctgcttgaca cggcggcacc gggccgataa
     9841 cgtccaagct cgcagcgatg cagaacgttt ccctcgaccg tctccggcag gtggcgaatc
     9901 tggcccg
//
LOCUS       DASUFA010000402         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_189855, whole genome shotgun sequence.
ACCESSION   DASUFA010000402 DASUFA010000000
VERSION     DASUFA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189855"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1315
                     /gene="queA"
                     /locus_tag="VFX87_14575"
     CDS             278..1315
                     /gene="queA"
                     /locus_tag="VFX87_14575"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX4996205.1"
                     /translation="MDVALFDYELPAELIAQEPAEPRDASRLLVLDRTRGAWEDRAFG
                     DLPDLLRPGDCVVANRSRVIPARLLGTAVEGGRPVELLLLRPFAEGRWEALVRPGRRC
                     RVGARVELAGGAARARIVGEGTAGARVVEVEGPWPVRELLERHGLPPLPPYISRHDAP
                     KPEDRERYQTVYARDEGSVAAPTAGLHFTPELLARLKRRGVAVHYLTLHVGPGTFRPL
                     RARRVDEHRMEAEPIELPEATARAVHEAKRDHRRVVAVGTTTTRALEWAMGEDGRLRE
                     GAGEADLFIRPGHRFRVVDALITNFHLPRSTLLVLVAALAGRELILDAYRHAVAARYR
                     FYSYGDAMLIQ"
     gene            1312..2475
                     /gene="tgt"
                     /locus_tag="VFX87_14580"
     CDS             1312..2475
                     /gene="tgt"
                     /locus_tag="VFX87_14580"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX4996206.1"
                     /translation="MSAGTFGFELIRTDGAARLGRMRTAHGSVDTPAFMPVATQGTVK
                     SLTPADLTAAGAQIVLANTYHLLLRPGHELVRELGGLHRFMAWDGPILTDSGGFQVWS
                     LSNLRRIGEEGVEFRSHVDGTLRTLTPESVVAVQHALGVDVLHPLDECLEHPATLAET
                     ERSLALTLRWLRRAVAAHRAAGAPGALFGIVQGGTSHAMRRRAVEETCALDLPGYAIG
                     GLAVGEPKPELYDIAELVAAALPGDRPRYLMGVGKPGDLVEAVARGVDLFDCVMPTRN
                     ARNGQAFTPDGPLNIENARFRHDGGPLDAACPCEACRRFSRAYLRHLFAARELLGYRL
                     LSLHNLTFYLRLMRQMREAVARDDFASFRSRFLERYGVESLRGSADIPADSEA"
     gene            2481..2819
                     /gene="yajC"
                     /locus_tag="VFX87_14585"
     CDS             2481..2819
                     /gene="yajC"
                     /locus_tag="VFX87_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX4996207.1"
                     /translation="MIDFAYAMAPSPGGGGNGGGIMSVLPLMVGMFAIMYFLIIRPQQ
                     KQRRERENLLRAIKKGDRVVTTGGLYGTVVGLSEHTVTLKVADQVKLDFERGSIGRIV
                     PAAGDKESSA"
     gene            2821..>3855
                     /locus_tag="VFX87_14590"
     CDS             2821..>3855
                     /locus_tag="VFX87_14590"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HEX4996208.1"
                     /translation="MRRQLGARIALVVVVIAVSVWYLYPPAKTINLGLDLQGGIHLVL
                     GVDVDKALEAQVERAGDTAKAELEKRGIGVTRIERRGTTELAIQLASPQSWDAAVKTS
                     DEVLPTFDRKEADQAAGRLVLALKLREAVHIRELAVRQGLETIRNRIDQFGVAEPSIQ
                     QQGDNRILVQLPGVQDPARAKALIGKTALLEFKLVDERTDVDTALRSGPPPGDEILYQ
                     RRVDKQTREERKVPFLVQKKAAITGRDVTTARVSIDENTSEPYVSVDFNAAGARAFSD
                     LTDANVGKRLAIVLDGNVHSAPQIRERIPSGRAQITGGFSTDEATDLAIVLRAGALPA
                     PVQVLEERTVG"
BASE COUNT          512 a         1339 c         1429 g          575 t
ORIGIN      
        1 ggcggggcgg cgggtggggc ggcgggcggc aggtgggcct ctgatcacga cccgctacct
       61 ggtcctcttg cgtgaggagt tcccaatctg ggatgcgggc gttccgggct tccgtcggtg
      121 gggaacggat cacccggccc acccgccgcc ccccgcccca cccgccgccc cggtctccgt
      181 ctttggcctt tctccacagg ctctcttcgc ggtctcttcg tttccccggg ttctccggct
      241 ggctcggggc gcgggtgggg gctacacttc gtgggctatg gacgtggcgc tgttcgacta
      301 tgagctgccg gccgagctga ttgcgcagga gccggcggag ccgcgggatg cttcgcggtt
      361 gctcgtgctc gaccgcacac gcggtgcgtg ggaggaccgg gccttcggtg atcttcctga
      421 cttgctgcgg ccgggggatt gtgtggtcgc caaccggagt cgggtgattc cggcgcggtt
      481 gctgggcacc gcggtcgagg gcgggcggcc cgttgaactc ttgctgctgc ggccgttcgc
      541 cgaggggcgg tgggaggcgc tggtgcggcc cgggcggcgg tgtcgggtgg gggcgcgggt
      601 ggagctggcg gggggagccg cgcgggcgcg gatcgtggga gagggaaccg cgggggcgcg
      661 ggttgtcgag gtcgaggggc cgtggccggt gcgggagctg ctggagcggc atgggttgcc
      721 gccgctgccg ccgtacatca gtcggcacga tgcgccgaag ccggaggacc gtgagcgcta
      781 tcagacggtc tacgcgcgcg acgagggctc ggtggcggcg cccaccgcgg gtctgcactt
      841 cacgccggag ttgctggcgc ggctgaagcg ccggggtgtc gcggtgcact acctgacgct
      901 gcacgtgggg ccgggcacgt tccggccgct gcgggccagg cgggtcgacg agcaccgtat
      961 ggaggcggag ccgatcgagc ttcccgaggc gaccgcgcgc gccgttcacg aggcgaagcg
     1021 cgaccaccgc cgcgtcgtcg cggtgggaac caccacgacg cgggcgctcg agtgggcgat
     1081 gggcgaggac gggcggctgc gcgagggagc gggcgaggcc gatctcttca tccggcccgg
     1141 ccaccgtttc cgcgtggtgg acgcgctcat caccaacttc cacctgccgc gctcgaccct
     1201 gctcgtcctg gtggcggcgc tcgcggggcg ggagctcatc ctggacgcat accggcacgc
     1261 ggtcgccgcg cgctaccgct tttactcgta cggcgacgcc atgctgatcc agtgagcgcg
     1321 gggaccttcg gcttcgagct gatccggacc gacggcgccg cgcgcctcgg ccgcatgcgc
     1381 accgcgcacg ggtcggtgga tacgcccgcc ttcatgccgg tggcgacgca gggcacggtc
     1441 aagagcctga ccccggccga tctcaccgcg gccggcgccc agatcgtgct cgccaatacc
     1501 taccatctgc tcctgcgccc gggccacgag ctggtccgcg agctgggcgg gctgcaccgc
     1561 ttcatggcct gggacgggcc gatcctgacc gactcgggcg gcttccaggt ctggagcctc
     1621 tccaacctgc ggcgcatcgg ggaagagggt gtcgagttcc gctcccacgt cgacgggacg
     1681 ctccgcacgc tcacgccgga gtcggtcgtg gccgtccagc acgcgctcgg cgtcgacgtg
     1741 ctccacccgc tggacgaatg cctcgagcat ccggccaccc tggccgagac cgagcgctcg
     1801 ctcgcgctga ccttgcgatg gctccgtcgc gccgtggccg cgcatcgggc ggcgggcgcg
     1861 ccgggcgcgc tcttcggcat cgtgcagggc ggcacctcgc acgcgatgcg gcgccgcgcg
     1921 gtggaggaga cctgcgcgct cgacctgccg ggctacgcca tcggcgggct cgcggtgggc
     1981 gagccgaagc ccgagctgta cgacatcgcc gagctggtgg ccgcggcctt gcccggcgac
     2041 cgcccgcgct acctgatggg cgtgggcaag cccggggatc tcgtggaggc ggtggcccgc
     2101 ggcgtcgatc tcttcgactg cgtgatgccg acgcgcaacg cgcgcaacgg tcaggccttc
     2161 accccggacg ggcccctcaa catcgagaac gcccgcttcc ggcacgacgg cgggccgctc
     2221 gacgccgcct gcccgtgcga ggcgtgccgc cggttctcgc gggcctatct tcgccacctc
     2281 ttcgccgcgc gcgagctcct cggctaccgg ctcctgtcgt tacacaacct caccttctac
     2341 ctgaggctca tgcggcagat gcgcgaggcg gtggcgcgcg acgactttgc ctcgttccgg
     2401 tctcggtttc tggagcgtta tggggtagaa tccctgcgcg gctcggccga cattcccgcg
     2461 gactcggagg cctgattttc atgatcgatt tcgcatacgc gatggcgccg tcgcccggcg
     2521 gcggaggcaa cggcggcggc atcatgtccg tcctcccgct gatggtcggc atgttcgcca
     2581 tcatgtactt cctgatcatc cgcccccagc agaagcagcg gcgcgagcgc gagaacctcc
     2641 tgcgggcgat caagaagggg gaccgcgtcg tgaccaccgg cggactttac ggcacggtgg
     2701 tggggctatc cgagcacacc gtgacgctca aggtggccga tcaggtgaag ctcgacttcg
     2761 agcggggctc catcgggcgc atcgtccccg ccgcgggcga caaggagtcg agcgcctagg
     2821 atgcgtcgcc agctcggagc ccgcatcgcc ctcgtcgtgg tcgtcatcgc ggtgtcggtc
     2881 tggtacctgt acccgcccgc caagaccatc aacctgggcc tcgacctcca gggcgggatc
     2941 catctcgtcc tcggcgtgga cgtggacaag gcgctggaag cccaggtcga gcgggcaggg
     3001 gacaccgcga aggccgagct ggagaagcgc gggatcgggg tcacgcggat cgagcggcga
     3061 ggcaccaccg agctcgccat ccagctcgcc tccccgcaga gctgggacgc ggcggtgaag
     3121 acgagcgacg aggtcctgcc caccttcgac cggaaggaag ccgaccaggc cgccggccgc
     3181 ctggtgctgg cgctcaagct gcgcgaggcc gtgcacatcc gcgagctggc ggtgcggcag
     3241 gggctcgaga ccatccgcaa ccgcatcgac cagttcggcg tggccgagcc gtccatccag
     3301 cagcagggtg acaaccgcat cctggtgcag ctgccgggcg tgcaggatcc ggcgcgggcc
     3361 aaggccctca tcggcaagac cgcgttgctc gagttcaagc tggtggacga gcgcaccgac
     3421 gtggacaccg cgttgcgaag cggcccaccc cccggcgacg agatcctcta ccagcgccgg
     3481 gtggacaagc agacccgcga ggagcggaag gtgccgttcc tcgtccagaa gaaggcggcg
     3541 atcaccgggc gggacgtgac caccgcgcgg gtctcgatcg acgagaacac gagcgagccc
     3601 tacgtctcgg tggacttcaa cgccgccggg gcccgggcct tctcggacct gaccgacgcc
     3661 aacgtgggca agcgcctcgc catcgtgctc gacggcaacg tccactcggc cccgcagatc
     3721 cgcgagcgca ttccctccgg gcgggcccag atcacgggcg gcttctccac ggacgaggcc
     3781 accgacctcg ccatcgtgct gcgggcgggc gcgctgccgg cgccggtcca ggtgctcgag
     3841 gagcggacgg tcggg
//
LOCUS       DASUFA010000403         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1241886, whole genome shotgun sequence.
ACCESSION   DASUFA010000403 DASUFA010000000
VERSION     DASUFA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241886"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFX87_14595"
     CDS             <1..187
                     /locus_tag="VFX87_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4996209.1"
                     /translation="GMALNHATPAQLAGIHAALVEGLRNGSLRPVIALELPLAQAARS
                     HEAVMEAGHHGKVVLIP"
     gene            complement(251..1018)
                     /locus_tag="VFX87_14600"
     CDS             complement(251..1018)
                     /locus_tag="VFX87_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4996210.1"
                     /translation="MSTTEQAIDAFWAAARNRTAPPAEWMNRLSRDEAYRVQLGVLAR
                     HVAAGERQAGWKVGLTSKAMQIQQKAHEPVLGFLLASGEQPSGTRFRFVDLISPGFEN
                     ELCLTVGRTLRGPGVTNAQAQAALTAMAPAFEIIEKRGDFAADLNVSLADNAQQRAYV
                     TGAAVPVPAGFDLAGVVLELSVNGGAPDRATGAEVLGTPAASIAWLANKLAEFGLALE
                     AGMKVMSGSFTRQYAITRGDRIAATFTPVGPVRAEFV"
     gene            complement(1015..>2798)
                     /locus_tag="VFX87_14605"
     CDS             complement(1015..>2798)
                     /locus_tag="VFX87_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4996211.1"
                     /translation="IVVDPRRAGAAARADQWLRVRPGTDGALALGLAAVMIAEGWYDR
                     EFVRDWSNGSFLVRADTGRLLTAADLSAVGSPAHFVAWDRAAGRPVAYDPAVADFIEP
                     IGDPQLDGSVRCMTRSGPVTCVPAFETYAALCREYPPERVERITGVPAAQLVETARLL
                     WERRPVSYFHWTGLEQHTNASQTVRALSLLYALTGCFDAPGGNVRPSRPTVNDLAPLA
                     LLPERQRAKALGLAQRPLGPARQGWPTAADAYRAMLHGAPYPVRGMVGFGTNLLLSAP
                     GAATARAALTSLDFLVYADLFLTPTAALADVVLPVSTAWEREGLRVGFGPTQDGEQHV
                     QLRPAVVAPRGEARSDIWIVCELARRLGFGERFWGGDEDAGHRFMLEPTGVTLEQLRE
                     NPGGVRTTIGPRFKRYAARGAGGPVGFATPSRRVEIFSAQLQQYGQSPLPEYVEPAAS
                     PVSRPDLAAQFPLQLTTAKVVQFCHSQHRSLARLRRHSPDPQVELHPAAAEARGITSD
                     DWVLVETPRATMRARARLNPSLAPDVVCAQFGWWQACEPLQLGGYPAEGAASANYNNL
                     IDPDTADPISGTVALRSYVCQVRRGEP"
BASE COUNT          368 a         1023 c         1058 g          349 t
ORIGIN      
        1 cggcatggcg ctcaaccacg ccactcccgc ccagctggcg ggcatccacg ccgcgctcgt
       61 cgagggcctg cgcaacggct cgctgcgccc ggtcatcgcc ctggagctgc cgctggcgca
      121 ggccgcgcgc agccacgaag cggtgatgga agcaggccac cacggcaagg tcgtcctcat
      181 cccgtaggag cccctcaccc tgggccccct caccctgccc tctccccctg gtcgggggag
      241 agggtggcgg ctagacgaac tcggcgcgga ccgggccgac cggggtgaag gtcgcggcga
      301 tgcggtcgcc gcgcgtgatg gcgtactggc gggtgaacga gcccgacatc accttcatgc
      361 ccgcttccag cgccaggccg aactcggcga gcttgttggc gagccaggca atggaggccg
      421 cgggcgtgcc cagcacctcc gcgccggtgg cgcggtcggg ggcgccgccg ttcacggaga
      481 gctccagcac gaccccggcc aggtcgaagc ccgcgggcac cggcaccgcc gcgcccgtca
      541 cgtaggcgcg ctgctgcgcg ttgtcggcca ggctcacgtt cagatcggcc gcgaagtcgc
      601 cgcgcttctc gatgatctcg aaggccggcg ccatcgcggt cagcgcggcc tgggcctgcg
      661 cattcgtgac cccgggcccg cgcagcgtgc ggcccaccgt gaggcagagc tcgttctcga
      721 agccgggact gatcagatcc acgaaccgaa agcgcgtgcc gctcggctgc tcgccactgg
      781 ccagcaggaa gcccagcacc ggctcgtgcg ccttctgctg gatctgcatc gccttcgagg
      841 tgaggccgac cttccagccg gcctgccgct cgcccgccgc cacgtggcgc gcgagcacac
      901 cgagctgcac gcgataggcc tcgtcgcggc tcaggcggtt catccactcg gccggcggag
      961 cggtccgatt gcgggccgcc gcccagaacg cgtcgatggc ttgctcggtg gtgctcatgg
     1021 ctctccccgg cggacctggc agacatagga ccgcagcgcg accgtcccgc tgatcgggtc
     1081 ggcggtgtcg ggatcgatca ggttgttgta gttggcgctg gccgcgccct ccgccgggta
     1141 gcccccgagc tggagcggct cgcacgcctg ccaccatccg aactgcgcgc agacgacgtc
     1201 gggggccagg ctcggattga gccgggcccg cgcccgcatg gtggcgcgcg gtgtctcgac
     1261 cagcacccag tcgtcgctcg taatgccgcg ggcctcggcc gcggccgggt gcagctcgac
     1321 ctgcgggtcg gggctgtgcc ggcgcaggcg ggccaggctc cggtgctggc tgtggcagaa
     1381 ctgcacgacc ttggcggtgg tgagctgcag cggaaattgc gccgcgaggt cgggccggct
     1441 gaccgggctc gccgcgggct cgacgtactc gggcagcggt gattgcccgt actgctggag
     1501 ctgcgcggag aagatctcca cccggcggct cggcgtggcg aagccgaccg ggccgccggc
     1561 gccgcgagcc gcgtagcgct tgaagcgagg cccgatcgtg gtgcgcacgc cgccgggatt
     1621 ctcgcggagc tgctcgagcg tgacgccggt gggctccagc atgaagcggt ggcccgcgtc
     1681 ctcgtcgccg ccccagaacc gctcgccgaa cccgaggcgc cgggccagct cgcagacgat
     1741 ccagatgtcc gagcgtgcct cgccccgcgg cgccaccacc gcgggccgga gctggacgtg
     1801 ctgctcgccg tcctgcgtcg gcccgaagcc cacgcgcagg ccctcgcgct cccacgcggt
     1861 ggacaccggc agcaccacgt ccgccagcgc cgcggtgggc gtcaggaaga gatcggcgta
     1921 gaccaggaag tcgaggctgg tgagcgcggc gcgcgcggtg gccgcgccgg gcgcggagag
     1981 cagcaggttg gtgccgaagc cgaccatgcc gcgcaccggg tagggcgcgc cgtggagcat
     2041 ggcgcggtag gcgtcggccg cggtgggcca gccctggcgc gcgggcccga gcggccgctg
     2101 ggccaggccg agggccttcg cgcgctgccg ctccgggagc agcgcgagcg gggccaggtc
     2161 gttgaccgtc gggcggctcg gccgcacgtt gccgccgggc gcgtcgaagc agccggtgag
     2221 cgcgtagagc agcgagagcg cgcgcacggt ctggctcgcg ttggtgtgct gctcgagccc
     2281 ggtccagtgg aagtaggaga ccgggcggcg ctcccacagg agccgcgcgg tctcgacgag
     2341 ctgggcggcc ggcacgccgg tgatgcgctc cacgcgctcg ggcgggtact cgcggcagag
     2401 cgcggcgtag gtctcgaagg ccggcacgca cgtcacgggg cccgagcgcg tcatgcaccg
     2461 cacggacccg tcgagctgcg ggtcgccgat cggctcgatg aagtcggcga cggccggatc
     2521 gtacgccacc ggccggcccg ccgcgcgatc ccatgcgaca aagtgcgcgg gactgcccac
     2581 cgccgagaga tcggcggcgg tgaggaggcg gccggtgtcg gcgcgcacca ggaaggagcc
     2641 gttcgaccag tcccggacga actcgcggtc gtaccagccc tccgcgatca tgaccgcggc
     2701 gagcccgagc gcgagcgcgc cgtcggtgcc cgggcgcacg cgcagccact gatccgcccg
     2761 ggccgccgcc ccggcgcgtc gcggatccac cacgatca
//
LOCUS       DASUFA010000404         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_439491, whole genome shotgun sequence.
ACCESSION   DASUFA010000404 DASUFA010000000
VERSION     DASUFA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439491"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFX87_14610"
     CDS             complement(<1..374)
                     /locus_tag="VFX87_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX4996212.1"
                     /translation="MLATANAAKARELAAGLAGIPYRLLALADFPGVTLPPEGGDSYT
                     ENALAKARAAAAATGALALADDSGIEVDALDGAPGVLSARYGGEGRSDAERCATMLEA
                     LKGVPPLQRSARYRCVIAIAEPG"
     gene            complement(385..1137)
                     /gene="rph"
                     /locus_tag="VFX87_14615"
     CDS             complement(385..1137)
                     /gene="rph"
                     /locus_tag="VFX87_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX4996213.1"
                     /translation="MISLPLPRHDGRVPDQLRRVILTSDYLRHPEGSVLVEFGDTKVI
                     CTASVEEKVPAFLKGQAKGWVTAEYGMLPRSTNTRMSRERGGPSGRSQEIQRLVGRSL
                     RSVVDMPKLGERTVWMDCDVIQADGGTRTAAITGSWVALSDALDRLVQAGTLPGSPLR
                     ENVAAISVGMVGGRALLDLDYAEDSTAEVDMNVVMTGSGAFVEVQGTAEQTPFGKDRL
                     AELLALAERGIDALVGLQRRAVATRGEKSFAL"
     gene            complement(1134..>2562)
                     /locus_tag="VFX87_14620"
     CDS             complement(1134..>2562)
                     /locus_tag="VFX87_14620"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX4996214.1"
                     /translation="PVSPDEGGSYLAAERLAAVLKGTWSVKGPRGTLTVGSRSAQFTR
                     NQARVLVAGDALTLDAPARTGTSGWLIPEDFLAKGLTRLAPGLTMKVVAVPASAPKAT
                     ARPVAAAAPTIAFEDLRLRSYPSFTRIVLEMGAPVGYVIVDESAERKEIRMRLPGLRL
                     AGPQLQEIDDGLVKELRLDPASGGAVLRIILEAPAGQVKEFALQDPARIVLDLYRTKE
                     GAGPEGAPGGAAAQPLRLVVLDAGHGGHDPGATGPTGVQEKDVVLDVTRRVARMMEES
                     GLGIKVVLSRTGDVFVPLRDRTNFANKQRADLFVSIHANAHPRSVSEGVETYFLSSEA
                     SDTESRQTAAIENGVVQLESPASQRKTDAIKSILWDLAQSEFQQESSFLAETVLDSMT
                     RSLRLVNRGVKQAGFFVLGGAAMPAILIEIGFLTNPKEERKLATAEYREATARAIFTG
                     LNEYKRRYDQRMRTALARPGGAAPR"
BASE COUNT          363 a          914 c          922 g          363 t
ORIGIN      
        1 ccgggctcgg cgatggcgat gacgcagcga tagcgcgcgg agcgctggag cggcggtacg
       61 cccttgagcg cctcgagcat cgtcgcgcac cgctctgcgt ccgaccgccc ctcaccgcca
      121 tagcgggcgg agagcacgcc cggcgcgccg tcgagcgcgt ccacctcgat gccggaatcg
      181 tcggcgagcg cgagcgcgcc ggtcgccgcc gcggccgctc tggccttggc gagggcgttc
      241 tccgtgtagg agtcgccgcc ctcgggaggc agggtcacgc cgggaaagtc ggcgagggcg
      301 agcaggcggt aggggatgcc ggccagcccg gccgccagct cgcgagcctt ggcggcgttg
      361 gcggtggcca gcaccagcgc ggcgtcagag ggcgaaggac ttctccccgc gcgtggccac
      421 cgcgcgacgc tgcaacccca ccagcgcgtc gatgccgcgc tcggccaggg ccaggagctc
      481 ggcgagcctg tccttgccga agggcgtctg ctcggccgtc ccctgcacct cgacgaatgc
      541 ccccgagccc gtcatcacca cgttcatgtc cacctcggcg gtggagtcct ccgcatagtc
      601 gagatcgagc agcgcccggc cgcccaccat gcccacgctg atcgcggcca cgttctcccg
      661 cagcggcgac ccgggcagcg tgcccgcctg gacgagccgg tccagcgcgt cggagagcgc
      721 cacccacgag ccggtgatgg cggcggtgcg cgtgccgccg tcggcctgga tcacgtcgca
      781 gtccatccac accgtgcgct cgccgagctt gggcatgtcc acgaccgatc ggagcgagcg
      841 cccgacgagt cgctggatct cctgcgagcg cccgctgggg ccgccgcgct cgcgactcat
      901 ccgggtattg gtcgagcgcg gcagcatgcc gtactcggcg gtcacccagc ccttggcctg
      961 ccccttgagg aaggccggca ccttctcttc caccgaggcg gtgcagatca ccttggtgtc
     1021 gccgaactcg acgagcaccg acccttccgg atggcgcaga tagtcgctgg tgaggatgac
     1081 ccggcggagc tggtccggca ctcggccgtc gtgacgcgga aggggaagcg agatcatcga
     1141 ggggcggctc cgccgggccg ggcgagcgca gtgcgcatgc gctggtcgta gcggcgcttg
     1201 tattcgttga gcccggtgaa gatggcccgc gccgtcgcct cgcgatactc cgccgtggcc
     1261 agcttccgct cttccttcgg attggtcagg aagccgatct cgatgaggat ggcgggcatg
     1321 gcggcgccgc ccagcacgaa gaagcccgcc tgcttgaccc cccgattgac gaggcggagg
     1381 gagcgggtca tcgagtccag cacggtctcg gccaggaagc tcgactcctg ctggaactcg
     1441 gactgggcga ggtcccagag gatgctcttg atcgcgtcgg tcttccgctg cgaggccggg
     1501 gactcgagct ggaccacgcc gttctcgatg gccgcggtct ggcgcgactc ggtgtcgctg
     1561 gcctcggagg agaggaaata ggtctcgacc ccctccgaca ccgagcgggg atgggcgttg
     1621 gcgtggatgg acacgaagag gtcggcgcgc tgcttgttgg cgaaattggt gcggtcgcgc
     1681 agcggcacga agacgtcccc ggtgcggctc agcaccacct tgatgccgag cccgctctcc
     1741 tccatcatcc gcgccacacg ccgggtgacg tcgagcacca cgtccttttc ctggaccccg
     1801 gtggggccgg tggcccccgg gtcatgcccg ccatggccgg cgtcgagcac cacgagacgc
     1861 agcggctgcg cggcggcgcc gcccggggcg ccctccggac ccgcgccttc cttggtgcgg
     1921 tagaggtcca gcacgatgcg agcgggatcc tggagcgcga actccttcac ctgcccggcg
     1981 ggggcctcca ggatgatccg gagcacggcg ccgccgctcg ccgggtcgag ccgcagctcc
     2041 ttcaccagcc catcgtcgat ctcctgcagc tgcggcccgg caagccggag gcccgggagc
     2101 cgcatgcgga tctccttgcg ctcggcgctc tcgtccacga tgacgtaccc gaccggtgcc
     2161 cccatctcca gcacgatgcg ggtgaaggac ggataggagc gcagccgcag atcctcgaaa
     2221 gcaatcgtag gagcggcggc ggccaccggc cgggccgtgg ccttcggcgc gctggccggc
     2281 acggccacca ccttcatggt cagcccgggg gcgaggcgag tcagtccctt ggcgaggaag
     2341 tcctcgggga tgagccagcc cgaggtcccc gtccgggcgg gagcatccag ggtcagcgca
     2401 tccccggcca cgaggacacg cgcctggttg cgggtgaact gggccgagcg ggaacccacc
     2461 gtgagcgtgc cgcgcggccc cttgaccgac caggtgccct tgagcaccgc ggcgaggcgc
     2521 tcggcggcga gataagagcc gccttcatcg ggacttacgg gg
//
LOCUS       DASUFA010000405         7733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_190727, whole genome shotgun sequence.
ACCESSION   DASUFA010000405 DASUFA010000000
VERSION     DASUFA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190727"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFX87_14625"
     CDS             <1..201
                     /locus_tag="VFX87_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4996215.1"
                     /translation="TDRVGAVPYNDTLSLRRAERVRDELVKVGIAAERIRVAGRGERE
                     PLVATPDEVAEPRNRRVEINVR"
     gene            complement(291..2378)
                     /locus_tag="VFX87_14630"
     CDS             complement(291..2378)
                     /locus_tag="VFX87_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX4996216.1"
                     /translation="MRALRGGRGRPVGVLLLALLLVLALLPEVPGLRLLRLAAFDGYQ
                     NLAPRVRQSMPVVIVAIDEESLQRHGQWPWPRTWLARLVTRIAEARPAAIGLDIMMPE
                     PDRLSPERVPSLVDGLDQELARKLAGMPRNDTVLAAALRGRPVVLGMAGLESVPGPPA
                     GIGQAPVRAFGGDPAPFVRRFAGALRSVDEIDAAATGRGLISVDPERGVVRRMPLLAS
                     LGGTLAPVLGLEMLRVAANVPALSVRVGAGGLEAVAVGDLVVPTEPDGRVWLHYSRHD
                     PSRFVSAADVLGGQIAADRFEGRLVLIGATALGLSDYQATPVADRMAGVEIHAQFLEA
                     VVDGTLLSRPPLARWIEIGLLVAGGLLLVLAVPLLRARTSLALIVLLVAATLALGFVL
                     YARRGILLDAATPALALTALFTVMLAATLTEAESQRRALRRQVEQQREAAARLAGELE
                     AARRIQMGTLPRPAAAFPGETRFDLYAMLEPAREVGGDLYDFFFLDADHLFFMIGDVS
                     GKGLPGSLFMAISKALYKSTALRRHGQVAVMMREADAEISRDNAEGLFVTVLAGILDA
                     RSGELEYCSAGHEPPLLLPRGGRALLRLAEGGGPPLCAVDGFPYVPATRRLEPGDTLC
                     LITDGITEAAGPDGQLYGRERLEALLGRLGGTASAAEVGEAIRRDVAAFAGGTEPSDD
                     MAVLVLRYTGAPS"
     gene            complement(2452..3408)
                     /locus_tag="VFX87_14635"
     CDS             complement(2452..3408)
                     /locus_tag="VFX87_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4996217.1"
                     /translation="MRVRFWGTRGSIPVAMTSAEVQRKLVTALVAAAGRGLDTAGKAR
                     AFVENELDFSVSHTFGGNSSCVQLEMDGPDYVLCDLGSGARVFGNSLLATRGPSGHRF
                     HVFMSHLHWDHIMGFPFFMPAYLPGNHVTIYGCHDTLEEAFRRQNRAPSFPVDFSRME
                     ARIEFVRLEPERNYDIAGLRVRAKLQHHGGDSYGYRIERRGKAVVYSTDSEHKQDDPE
                     EVKAFVEFFRDADLVIFDAQYSLADAISVKEDWGHSSNVVGVEMCQLARARRLCLYHH
                     EPIFDDERIARLVAETRRLEEITRTDHRVEVWAAYDGLEIAL"
     gene            complement(3418..3792)
                     /locus_tag="VFX87_14640"
     CDS             complement(3418..3792)
                     /locus_tag="VFX87_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4996218.1"
                     /translation="MELAAKRYADIVALYPAGRIDHATADAFKAALAPHLAGVASGRD
                     RAVIDLAGVEYISSVGLRVLMLASKQVKAQGGALAVADLQPVVREIFEISRFNLVLEV
                     FPTLREALAKLSPAGLAAFESA"
     gene            3856..5109
                     /locus_tag="VFX87_14645"
     CDS             3856..5109
                     /locus_tag="VFX87_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4996219.1"
                     /translation="MAHWDVLEKSDLLGQLRIDRGAARPPHRRPRLYVILAAAVLLLG
                     GGLWFGLLRARPLVVQTAVARAASQGAGAGSVLDASGYVTARRQATVSAKITGKVTEV
                     LIEEGQRVREGAVLARLDDTEAQAQLGLTRAQLVAARSQLAEVRAQLVQAELDHARQQ
                     ELYDRKLIAPQSLDAALAQRDMLRARLAATEEQVKVAQQAQAVAQVQLDNTVIRAPFS
                     GVVIAKSAQPGEMISPISAGGGFTRTGIGTIVDMDSLEIQVDVNESYINRVTPGQPVE
                     ATLNAYPDWKIPGEVIAIIPTADRSKATVKVRIAIKAKDPRIVPDMGARVGFLEPKPA
                     GGEPRQPPPGVLIPAEAVRTDNDRATVFVYNANGKVERRSVTLGPDVSGRRRVLSGLR
                     DGERVVLAPPPALADGQAVKLAEPR"
     gene            5115..5804
                     /locus_tag="VFX87_14650"
     CDS             5115..5804
                     /locus_tag="VFX87_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996220.1"
                     /translation="METLVEIRNVTKVYRRGRERIEVLHNIDLDIPRADFVALMGPSG
                     SGKTTLLNLIGGLDTPTGGSITVGGRRIDQLSGGELAAWRAGHVGFVFQFYNLLPALS
                     AQRNVELPLLLTDLSASERRRRARIALTLVGLEDRARHKPRELSGGQEQRVAIARAIV
                     SDPTLLVCDEPTGDLDRATADEVLRLIQMLNREHGKTVVMVTHDPKAAEYARRRIHLD
                     KGTMTAEPVSA"
     gene            5801..6964
                     /locus_tag="VFX87_14655"
     CDS             5801..6964
                     /locus_tag="VFX87_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4996221.1"
                     /translation="MRYLPLLWASLFRKKARTILTLLSIVVAFALFGLLQAVQVAFES
                     GADAADAKRLLTTARYSIIEPLPMSYGRRIEQVPGVVGVAYADWFGAKYQNESNAFPV
                     FAVDPTRYLDMYPEFTIAPAHREAFARTRTGAVAGKRLADRFGWKVGQKLPISSEIHP
                     KTDGSMSWEFDLVGTFDADDPAVRGNTDMVLINVAYFDEARQIGRGKTGWYIVRVADS
                     AQARAISATIDGLFTNSPDETKTQPEKEFALGFAKQIGDIGALVTRILIAVFFTILIL
                     TGNTMAQSIRERIPELAILKTLGFSDGKVTALVLAEALLLLILGGGIGMGAAVAAMPA
                     LNGSTGGRFPPLFVDADTWFLAAAIAALVALCIGLPPALRANRLNIVDALSGH"
     gene            7159..>7733
                     /locus_tag="VFX87_14660"
     CDS             7159..>7733
                     /locus_tag="VFX87_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996222.1"
                     /translation="MNLRNLPSRVGTSLVVVVGIAGVVGVLVSVLAMSEGFRHTLAST
                     GRGDRVIMLRAGSDAELSSGVDRDQATLLAALPGVARDGAGRSLASAELVVMVDLPRK
                     GESHPNNVPFRGVQRAAFDIRDEARLVEGRRFEPGVREVVVGRKAASQFAGLAVGSHI
                     GFRDSDWTVVGIFESGGDVHESEIWADAEVA"
BASE COUNT         1168 a         2774 c         2766 g         1025 t
ORIGIN      
        1 accgaccggg tgggcgccgt gccttacaat gacacgctct cgctccggcg cgccgagcgg
       61 gtgcgcgacg agctggtgaa ggtcggcatc gcggccgaac gcatccgcgt agctgggcgc
      121 ggggaacgcg agccgctggt ggccacgccc gatgaggtgg cggagccgcg gaatcggcgg
      181 gtggagatca acgtgcggta agccccgtgg ggtagaccac ccctcaccct gccctctccc
      241 ctccggggag aggggatgcg aagcttcccc atcccctcgg ggcggaaacc tcaggagggg
      301 gcaccggtgt agcgcaggac gaggaccgcc atgtcgtcgg agggctcggt gccgccggcg
      361 aaggcggcca cgtcgcggcg gatcgcctcc ccgacctcgg ccgcgctcgc ggtgcccccg
      421 aggcggccca gcagggcctc gaggcgctcc cgcccgtaga gctgcccgtc gggcccggcc
      481 gcctcggtga tcccgtcggt gatcaggcac agcgtgtcgc cgggctcgag ccggcgcgtg
      541 gccggcacgt agggaaaccc gtccaccgcg cagagcggcg ggcctccgcc ttcggccagg
      601 cgcaggaggg cgcggccgcc gcgcggcagg agcaggggcg gctcgtggcc cgcgctgcag
      661 tactcgagct cgccgctgcg ggcgtccagg atgccggcca gcaccgtgac gaagaggcct
      721 tccgcgttgt cgcgcgagat ctccgcgtcg gcctcgcgca tcatgaccgc cacctggccg
      781 tgccgtcgca gggcggtgct cttgtagagc gccttgctga tcgccatgaa gaggctgccg
      841 ggcaggccct tgcccgagac gtcgccgatc atgaagaaga gatgatcggc atcgagaaag
      901 aagaagtcgt acaagtcgcc gcccacctcc cgcgccggct ccagcatcgc gtacagatcg
      961 aagcgcgtct cgcccggaaa ggcggccgcc gggcgcggga gcgtgcccat ctggatgcgg
     1021 cgcgccgctt ccagctcgcc ggccagccgc gcggcggcct cgcgctgctg ctccacctgc
     1081 cggcgcaggg cgcgccgctg gctctccgcc tcggtgagcg tcgccgcgag catcacggtg
     1141 aagagcgcgg tcagggccag ggcgggcgtg gccgcgtcca gcaggatccc gcgccgcgcg
     1201 tagaggacga agccgagcgc gagcgtcgcg gccaccagca gcacgatgag ggccagcgac
     1261 gtgcgcgccc gcagcagcgg caccgcgagc acgagcagca ggccgcccgc gaccagcagg
     1321 ccgatctcga tccagcgcgc gagtgggggc cgggagagca gcgtgccgtc caccaccgcc
     1381 tcgaggaact gggcgtggat ctcgaccccg gccatgcgat cggcgaccgg cgtggcctgg
     1441 tagtccgaga gaccgagcgc ggtcgcgccg atcagcacga ggcgaccctc gaagcggtcc
     1501 gccgcgatct gtccgccgag cacgtccgcg gccgagacga agcgcgacgg gtcgtggcgg
     1561 ctgtagtgca gccacacgcg cccgtcgggc tcggtgggca ccaccagatc gcccaccgcc
     1621 accgcctcga ggcctcccgc gcccacgcgc accgagagcg cgggcacgtt cgcggccacg
     1681 cgcagcatct cgaggccgag gacgggcgcg agcgtgccgc cgagcgaggc cagcagcggc
     1741 atgcgccgga ccacgccgcg ctcggggtcc acgctgatga gcccgcgccc cgtcgcggcg
     1801 gcgtcgatct cgtccacgct gcgcagcgcg cccgcgaacc gccgcacgaa cggcgccgga
     1861 tcgcccccga aggcgcgcac cggcgcctgg ccgatgccgg cgggcggccc gggtacgctc
     1921 tcgagcccgg ccatccccag gaccaccggc ctcccgcgaa gcgcggcggc cagcacggtg
     1981 tcgttgcgag gcatgccggc gagcttgcgg gcaagctcct ggtccaggcc gtcgacgagc
     2041 gagggcacgc gctccgggga gagccggtcg ggctcgggca tcatgatatc gagaccgatg
     2101 gcggcgggcc gcgcctccgc gatccgcgtg acgagccggg cgagccaggt gcgcggccag
     2161 ggccattgcc cgtggcgctg gaggctctcc tcgtcgatgg ccacgatgac gacgggcatg
     2221 gactggcgta cgcgtggggc gaggttctgg tagccgtcga aggcggccag gcgcaggagg
     2281 cggagcccgg gtacctccgg cagcagggcc aggaccagca gcagggccag caggagcacg
     2341 ccgaccggcc ggccgcggcc gccccggagg gcgcgcatcc agcgccgctc cgcccagtgc
     2401 gggcgtgcgg acggctcgga ggcgggaacg tccgcgctca ggcgaaaggc ctcagagcgc
     2461 gatctccagg ccgtcgtagg cggcccagac ctccacgcga tgatcggtcc gcgtgatctc
     2521 ctcgagccgg cgcgtctcgg ccaccaatcg cgcgatgcgc tcgtcgtcga agatcggctc
     2581 gtggtgatag aggcagagac ggcgggcccg cgcgagctgg cacatctcca cacccaccac
     2641 gttgctcgag tgcccccagt cctccttgac cgagatggcg tcggcgagcg agtactgggc
     2701 gtcgaagatc acgaggtcgg cgtcgcggaa gaattcgacg aacgccttca cctcctccgg
     2761 atcgtcctgc ttgtgctcgg agtcggtgga gtagaccacc gccttgccgc gtcgctcgat
     2821 gcggtagccg taggaatcgc cgccgtggtg ctggagcttc gcgcggacgc ggaggcccgc
     2881 gatgtcgtag ttccgctcgg gctccaggcg cacgaactcg atgcgcgcct ccatcctcga
     2941 gaagtccacc ggaaaggacg gggcgcggtt ctgccggcgg aacgcctctt ccagcgtgtc
     3001 gtggcagccg tagatcgtca cgtggttgcc cggcaggtac gcgggcatga agaacgggaa
     3061 gcccatgatg tggtcccagt gcaggtggga catgaagacg tggaaccggt gcccggaggg
     3121 cccgcgggtg gcgagcagcg agttgccgaa gacccgcgcg ccgctgccca gatcgcagag
     3181 cacgtagtcg ggaccgtcca tctcgagctg cacgcaggag gagttgcccc cgaaggtgtg
     3241 ggagaccgag aagtcgagct cgttctccac gaaggcgcgc gccttgcccg cggtgtcgag
     3301 cccgcgcccc gccgcggcca ccagcgcggt gacgagcttg cgctgcacct cggcagatgt
     3361 catggccacc gggatcgagc cgcgggtgcc ccagaagcgg acgcgcacgt gggatcctca
     3421 ggccgactcg aaggcggcca ggcccgcggg cgagagcttg gccagggcct cccgcagcgt
     3481 ggggaagact tccaggacca ggttgaaccg gctgatctcg aagatctcgc gcaccaccgg
     3541 ctgcaggtcc gccaccgcga gcgcgccgcc ctgggccttc acctgcttgc tcgcgagcat
     3601 gagcacgcgc agccccacgc tgctgatgta ctccacgccg gccaggtcga tcaccgcgcg
     3661 atcacgcccg gatgcgaccc cggccaggtg gggcgccagc gcggccttga aggcgtccgc
     3721 ggtcgcgtga tcgatccgtc ccgcgggata gagcgccacg atgtcggcgt agcgcttcgc
     3781 ggccagctcc atgtcggctc ccgggggatc gaatcgggtg gaatcggggg tccgattcta
     3841 acaccgggac cggctgtggc acactgggac gtcttggaga aatcagacct gctcggccag
     3901 ttgcgtatcg accgtggggc ggcccgcccg ccgcaccggc gcccccgtct gtacgtcatc
     3961 ctcgccgcgg ccgtgctcct cctgggcggc gggctctggt tcggcctcct ccgcgcccgg
     4021 cccctggtgg tgcagaccgc ggtcgcgcgc gcggccagcc agggcgcggg ggccggctcg
     4081 gtgctcgacg cgagcggcta cgtcaccgcc cgccgccagg ccaccgtctc cgccaagatc
     4141 accggcaagg tgaccgaggt gctgatcgag gagggccagc gcgtgcgcga gggcgcggtg
     4201 ctggcccggc tcgacgacac cgaggcgcag gcgcagctcg ggctcacgcg ggcccagctc
     4261 gtcgccgcgc gctcccagct cgccgaggtc cgggcccagc tcgtccaggc cgagctagac
     4321 cacgcgcgcc agcaggagct ctacgaccgc aagctcatcg cgccccagtc gctcgacgcg
     4381 gcgctggccc agcgcgacat gctgcgggcg cgcctggccg ccaccgagga gcaggtcaag
     4441 gtcgcgcagc aggcgcaggc ggtcgcccag gtgcagctcg acaacacggt gatccgcgcc
     4501 ccgttcagcg gggtcgtcat cgccaagtcg gcgcagcccg gcgagatgat ctcgccgatc
     4561 tcggcgggcg gcggcttcac gcgcaccggc atcggcacca tcgtggacat ggactcgctg
     4621 gagatccagg tggacgtcaa cgagtcctac atcaaccgcg tgacgcccgg ccagccggtg
     4681 gaggcgacgc tcaacgccta tccggactgg aagatccccg gcgaggtgat cgccatcatc
     4741 ccgaccgcgg accggagcaa ggccaccgtc aaggtccgca tcgcgatcaa ggcgaaggac
     4801 ccgcgcatcg tgcccgacat gggcgcacgg gtgggcttcc tcgagccgaa accggccggt
     4861 ggtgagccgc ggcaaccgcc gccgggcgtg ctgataccgg ccgaagcggt acgcaccgac
     4921 aatgatcgcg caaccgtctt cgtctacaac gccaatggca aggtggagcg ccggagtgtg
     4981 acactcgggc ccgacgtctc cggccggcgc cgcgtgctga gcggcctgcg cgacggcgag
     5041 cgggtcgtgc tcgccccgcc gcccgccctt gccgacggcc aggccgtcaa gctggccgag
     5101 cccaggtgag cccgatggag accctcgtcg agatccgcaa cgtcaccaag gtttaccggc
     5161 gcggccgcga gcgcatcgag gtgctccaca acatcgacct cgacattccg agggcggact
     5221 tcgtggccct gatggggccg tcgggctcgg gcaagaccac gctgctgaac ctgatcggcg
     5281 ggctcgacac gccgaccggc ggcagcatca cggtgggggg ccggcgcatc gatcagctct
     5341 cgggcgggga gctggccgca tggcgggccg gccacgtggg cttcgtcttc cagttctaca
     5401 acctgctccc cgcgctctcg gcccagcgga acgtggagct gccgctgctc ctgaccgatc
     5461 tctccgcgtc cgagcgccgc cgccgcgcgc gcatcgcgct gaccctggtg ggcctcgagg
     5521 atcgcgcccg ccacaagcct cgggagctgt cgggtggcca ggagcagcgc gtcgccatcg
     5581 cgcgcgccat cgtctccgat cccaccctgc tggtctgcga cgagccgacc ggggacctcg
     5641 accgtgccac cgccgacgag gtgctgcgtc tcatccagat gctcaaccgc gagcacggca
     5701 agaccgtggt gatggtcacc cacgatccca aggccgcgga gtacgcgcgg cggcgcatcc
     5761 acctcgacaa gggcaccatg accgcggagc ccgtctccgc gtgagatacc tcccgctgct
     5821 ctgggccagc ctcttccgca agaaggcgcg caccatcctc acgctgctgt ccatcgtggt
     5881 ggccttcgcg ctcttcggcc tcctgcaggc ggtgcaggtc gccttcgagt cgggcgccga
     5941 cgcggccgac gccaagcgcc tgctcaccac cgcgcgctac tccatcatcg agccgctccc
     6001 gatgtcctac gggcgccgga tcgagcaggt gccgggggtg gtgggcgtcg cctacgcgga
     6061 ctggttcggc gccaagtacc agaacgagtc caacgccttc ccggtcttcg cggtggaccc
     6121 gacacgctac ctcgacatgt acccggagtt caccatcgcg cccgcgcatc gcgaggcctt
     6181 cgcgcggacg cgcaccggcg cggtggcggg caagcgcctg gccgatcgct tcggctggaa
     6241 ggtcgggcag aagctcccga tcagctcgga gatccatccc aagaccgacg ggagcatgag
     6301 ctgggagttc gacctggtcg gcaccttcga cgccgacgat ccggcggtgc gcggcaacac
     6361 cgacatggtc ctgatcaacg tggcctactt cgacgaggcc cgccagatcg gccgcggcaa
     6421 gactggctgg tacatcgtgc gggtggccga ctctgcccag gcccgcgcca tctcggccac
     6481 gatcgacggc ctcttcacga actcgcccga cgagaccaag acccagcccg agaaggagtt
     6541 cgccctgggc ttcgccaagc agatcggcga catcggggcg ctggtcaccc gcatcctgat
     6601 cgcggtgttc ttcaccatcc tgatcctgac cggtaacacg atggcccagt ccatccgcga
     6661 gcgcatcccg gagctggcca tcctgaagac gctcggcttc tccgacggca aggtcaccgc
     6721 gctggtgctc gcggaggcgc tcctgctgct gatcctgggc ggtggcatcg gcatgggcgc
     6781 ggcggtggcc gccatgcccg cgctcaacgg ctcgaccggc ggccgcttcc ccccgctctt
     6841 cgtcgacgcc gacacctggt tcctcgccgc cgccatcgcg gccctcgtcg ccctctgcat
     6901 cggtcttccc ccggcattgc gcgccaaccg cctgaacata gtcgacgccc tctcaggcca
     6961 ctagaacaaa ggccgaacga gaaaaccgca tatgaggccg acaaccataa taggcagcac
     7021 gcggcgggac aatgcggtgg gggggcttgg gggaggagcg acgccgctcc cccccaataa
     7081 gaacaatgca gtacctgaaa cggtcgacag caagcgcggc caatgcgaac cctagctcag
     7141 atagcggcga tcacgctcat gaacctccgc aacctgccgt ctcgggtggg cacgtcacta
     7201 gtggtggtcg tgggcatcgc gggcgtggtc ggcgtactgg tctccgtgct cgcgatgtcg
     7261 gaggggtttc gccacacgct ggccagcacc ggacgtgggg atcgtgtgat catgctgcgc
     7321 gccggctccg acgcggagct gtcgagcggc gtcgatcgcg accaggcgac gctgctcgcg
     7381 gccctgcccg gggtggcccg tgatggggcg gggcgctcgc tcgcctccgc ggagctggtg
     7441 gtgatggtgg atctgccgcg caagggcgag agccacccga acaacgtgcc gtttcgcggc
     7501 gtgcagcggg ccgcgttcga catccgggac gaggcgcgcc tcgtggaggg tcgccgcttc
     7561 gagcccggcg tccgggaggt cgtggtggga cgcaaggcgg ccagccagtt cgcggggctc
     7621 gcggtcggct cgcacatcgg cttccgcgac agcgactgga cggtggtcgg catcttcgag
     7681 agcggcggcg acgtgcacga gtcggagatc tgggccgacg cggaggtggc cat
//
LOCUS       DASUFA010000406        12107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_532910, whole genome shotgun sequence.
ACCESSION   DASUFA010000406 DASUFA010000000
VERSION     DASUFA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532910"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFX87_14665"
     CDS             complement(<1..759)
                     /locus_tag="VFX87_14665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4996223.1"
                     /translation="MSDSFRDVAVEAALQAGAFLRSRFGARHDISYKGSPTNLVTEMD
                     RGAETIILDAIRARFPGHGVLAEESGARSGAEPHRWIVDPLDGTTNYAHGLPIYCVSI
                     ALELHGELAVGVLYDPSRDECFVAERGRGATLNGAPLRVSATPALGESLLGTSYPNDL
                     RAAPRNNLAEHAALIQRCRSVRSLGSAALGLAAVAAGRLDGYWEQRLGAWDIAAGALL
                     IREAGGTVTAVHGAPLDLAAPTIAASNGPIHSDLL"
     gene            822..1754
                     /locus_tag="VFX87_14670"
     CDS             822..1754
                     /locus_tag="VFX87_14670"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4996224.1"
                     /translation="MRIVVMGSGGTGGFFGAKLARAGEDVTFVARGAHLAALRAEGLR
                     IKSAVEGEWSVRAPAVERLDGLPPADLVLFCVKSFDTEEAAALIRPVVGADTGVLSIQ
                     NGVDNEEKLARVLGAGHVLGGAVRVFATIEAPGVIAHTFGGHLIFGEMDGRETARARA
                     LLAACRKASIPAELVSDVTRALWEKYVFLTTHAGMTALTRCPAGVLRAVPEVREVYRR
                     TVTEIVAIGRAAGVKLDDAMLEQGFKFLDTVAPNAFSSLHHDLVHGKRLELEALHGHA
                     VRLAERHHVPAPTVFAIYAALLPYRDGAPTAPAR"
     gene            1810..3684
                     /locus_tag="VFX87_14675"
     CDS             1810..3684
                     /locus_tag="VFX87_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996225.1"
                     /translation="MSSLRRLLPYLDRYRLRYLGGAACLLLATAFSLSIPWTVKNAVD
                     ALERDGTAARIGPYVLLILALAAAHGGARLGSRFAMLGAGQWVEHDIRADVYTRLLAL
                     PPAFYHRHRTGDLMSRVSNDISTLRMLAGFGTVMLIGTTLAFGGALTAMWLIDPWLTL
                     FALAPFPALVIIAKRFHHDAEVRATAVQEQLGALSAKVQENLTGMPVVRAYTMEAREI
                     AEFERLNGEYLTRNMALARIQSVSWPLMGLVSGVGVLIVMWLGGKAVVDGRISLGAFV
                     AFNGYLTQLAWPTIALGWTLASIQRGLAAMSRVTEVLDERAHDGVKVRPQDGLGGQPG
                     GDWGWRPHVGLGGSSDARSIECGGPEMAPALPQSADNRELLPGGPVEFKGLTFAYDDR
                     GPVLRDVSFTVPAGSVVAVVGPTGSGKSTLGTLLCRLYEPPRGTVWVGGQDVLDLPLG
                     RLRRSVGYVPQESFLFSRPLRDNVRLADEGAGPERLEAVARTAGLADDLGALPRGWDT
                     VVGERGLTLSGGQRQRVALARALVADPPFLVLDDVLASVDAAKEWEITRALRGAASGR
                     TTLLMTHRLKAAAEADAIVVLDEGRVVEQGRHADLLAAGGLYARLWRVQQLEDELANA
                     "
     gene            3705..5561
                     /locus_tag="VFX87_14680"
     CDS             3705..5561
                     /locus_tag="VFX87_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996226.1"
                     /translation="MREAEQEDEVLGKAYDARLMGRLWAFTRPHTRLVLATCALFPAV
                     ALLELSQPYLIKIAIDDHILRRDWAGLGGIAALFLLVLLALYALRAAQAYLTQLTGQR
                     VIHDLRAALFAHLQRQDARFYDRSPVGRLMTRVLNDGEAVQELFTSGIVALLGDVVVL
                     TGVVVIMLGMNWKLALVTFALVPLLVLAAAYFRVRARDSYRVVRTRLARMNAFLQESL
                     QGMSVIQLFAREARERETFAQLSGALRAGQFRSTFYDASLYATVEALGSAAVALLLWY
                     GGGQVAIGALTFGGLVAFLEYTGRFFLPIRDLGAKYTVMQAAMVSAERIFGLLDTHPS
                     ITSPVPADGSSGRQVQDEAPPKASRADTRKEAPAAAPPTPAVEFRNVWFAYEGEQWVL
                     RDCSFTLAPGERVALVGPTGEGKTTIARLMIRAYDVTRGRVLVEGVDVREWDLQTLRR
                     HVGLVPQEVFLFSGTVEDNLRVAGNGAVDRPEIERALEAANAERFVAALPRGLREELR
                     ERGLNLSQGQRQLLAIARAIIYNPRVLALDEATSSIDPESEAQVRVGLARLLEGRTSL
                     VIAHRLSTIQRADRILVLHRGEIREAGGHAELLAQGGLYSRLYELQFGLGRM"
     gene            5558..5902
                     /locus_tag="VFX87_14685"
     CDS             5558..5902
                     /locus_tag="VFX87_14685"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4996227.1"
                     /translation="MKAFHAAVYRLVGRIPRGRVATYGQIAALLGYPRAARAVGQAMK
                     HVPAHLPWHRVVNAQGGISLRRNVGSMLTQRLLLEQEGVPVRRGRIRLQQHRWAGPGR
                     TRRLSIAALERL"
     gene            5915..6943
                     /locus_tag="VFX87_14690"
     CDS             5915..6943
                     /locus_tag="VFX87_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4996228.1"
                     /translation="MRLGLNIGYSRSQLGIDLSLVQHADRLGFHSVWSAEAYGSDAVS
                     PLCWIAAQTSRIKVGTAIMQIPARTPTLTATTAMTIDQLSGGRFILGLGVSGPQVVEG
                     WHGVAFGKPLAKTREYVEIVRMVWRRERPVEHKGEYYQIPYAGPDATGLGKPLKSILH
                     GRADIPIYLAAIGPRNVALAAEIADGLLPVFFSPERMAVFTPSLEAGFKTAGGGKSLA
                     SFDIAPTVPIVLGPDAAACRAMVKPRLALYVGGMGARGKNFYNELVTRYGYEADARRI
                     QDLYLGGKKEEAAAAVPDALVDEVALCGPKERIRERLPRWKSSGITTLICGAVQAEAL
                     ELMAELVL"
     gene            6949..7443
                     /locus_tag="VFX87_14695"
     CDS             6949..7443
                     /locus_tag="VFX87_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX4996229.1"
                     /translation="MKPAVQKVQAAIAEHGLGREVIELEVHARTSQQAADALGVTVGQ
                     IVKSLVFTVDGRPLLVAASGANRVNERRLGEVAGGRIRRADPDTVKQATGYTIGGVPP
                     IGLASPLPVYVDRDLLGYELVYAAAGLPECVFPIAPDELVRATGGQVVDIKEAGSDSK
                     GARP"
     gene            7440..8201
                     /locus_tag="VFX87_14700"
     CDS             7440..8201
                     /locus_tag="VFX87_14700"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4996230.1"
                     /translation="MRLKDKVALITGAGSGIGQATAVLFAREGARVAVVDLREDAAKA
                     TAEQIEKSGGQALAIRADVSKSADNQAAIQQAVARWGRLDIFYANAGVPQFPTPVESV
                     DEAVFDQIMAVNVKGVFLAAKHLMPVFRKQKGGVLLITGSTSGLRPRPGVQCYSASKG
                     AVHTLAASLALEFSAFGARVVAIAPVATETPMLATFMGKKQVDEEGMTRYRGTVPLGR
                     LNKPEDLAAAALFLASDDAAMITGSVFPVDGGRCI"
     gene            8243..9163
                     /locus_tag="VFX87_14705"
     CDS             8243..9163
                     /locus_tag="VFX87_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996231.1"
                     /translation="MATFVECGATRVLIDPGAMLAPNRFNLAPAEEEWEALRRANDRI
                     SAYATRASLVFVSHYHEDHFRHDPGLYAGRPLWAKDAARHIEGRQAQRAATLWRALKG
                     SAEPLAAEGRRLETADAVLQASPPLGHGADGTALGYVVALTIEDRRAGTRFVHASDVQ
                     GPLSPVAAAYLARQRPTLLYLSGPPAYLESQIGADLIDRGIEHLLRIIDQTGCRVIFD
                     HYALRDAAYPERFRRLWETGRVVTAAGFLGLADAPLEARRVELWPGRRKPPMPVRPSR
                     ARGAAEAGGARPPARVTFNTRPGVRRVREG"
     gene            9168..9635
                     /locus_tag="VFX87_14710"
     CDS             9168..9635
                     /locus_tag="VFX87_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:9312..9314,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX4996232.1"
                     /translation="MSEGTAPGVGDPAPDFTLKTIGLRDVSLKSFRGRNVVLLFYPLD
                     WTPGUSTCMPAFDRRIKDFEAAHTQVLGISSDSPFSHENWAKSVGISNYPLLSDIHRT
                     AGRAYGVYWPDWNANVRASFVIDKQGTIRFVERYGRGELPDPDKMLAEVRKLP"
     gene            complement(9632..10534)
                     /locus_tag="VFX87_14715"
     CDS             complement(9632..10534)
                     /locus_tag="VFX87_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4996233.1"
                     /translation="MPDPGQTRAYLALTLISALWGSYPAFAKLALAHFPPYVLVSLRC
                     TLASAFLAVLLFRRGWEEFRELRASDLRTFAFLGFTGLFVSTGGTYLGIALSTAANGA
                     ILQAATPVMVALGARFYLKERLRRRQWAGVVASMAGVLLVITRGSWRAITHLDLMPGD
                     FILMISQAGWSAYTIYGKQVLAVHSPAVATTASYILGSAMLLPMTLVAAPFFPRPDFT
                     SQTAWLVVLYQAFLGAIAHVWWYEGVKAVGPSRAAIFMNLQPVVGVALAWAMLHEAVE
                     WPEIVGGALVLLGVALTTQAPTTA"
     gene            10558..11010
                     /locus_tag="VFX87_14720"
     CDS             10558..11010
                     /locus_tag="VFX87_14720"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4996234.1"
                     /translation="MADYILERRVWLPRPRAVVFEFFADPRNLEKLHPAWARPRWLTP
                     PPARLAAGTVLEFRAAGLPGRWRVIVREFDAPYRFVDAQLNGPFARWEHRHRFTESAG
                     GTWVEDRVTYRLPLGPLGRLIHAVGAGHQVRHLFDYRDRRLRDLFGGE"
     gene            complement(11032..12039)
                     /locus_tag="VFX87_14725"
     CDS             complement(11032..12039)
                     /locus_tag="VFX87_14725"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX4996235.1"
                     /translation="MAEHIPAADPKMSRRVIPGTIETVFEFDYSVQAEDMRSLYEKAK
                     RDQWNASRDIAWDTPEPTDGRVLSDELVDIHGSPIWARLSEKDRVDLNRRIAAWRLSV
                     LVYGEQGAMLACSQMVDIVQGTDEKFFQATQVMDEARHNEVLERYIQTRLGGLHYPMP
                     ENERVLFDAILTDTRWYIKTIALQLVAETFAVAIFKMMGEAAADPLLRQVCTRILQDE
                     SRHMGFGMLALPDIVREASDTERQEMEDFTCLALEKVLTGFFPVEAYRDIGFSRAQIE
                     EVKVYRRDVAARNDYAVYRKYFKRDMHASMVNNLARIGLLTERVRPRLSALGIELPAA
                     G"
BASE COUNT         1676 a         4115 c         4519 g         1797 t
ORIGIN      
        1 gagcaagtcg gaatggatcg ggccgttcga ggccgcgatg gtgggcgccg cgaggtcgag
       61 cggggctccg tgcacggccg tcacggtgcc gcccgcctca cgaatcagca gcgcgcccgc
      121 cgcgatgtcc caggcgccca gccgctgctc ccagtagccg tcgagccggc cggccgcgac
      181 cgcggcgagc ccgagcgcgg cggatcccag gctccgcacg ctgcggcatc gctggatgag
      241 ggcggcgtgc tcggcgaggt tgttgcgcgg ggcggcccgc aggtcgttcg ggtagctcgt
      301 gccgaggagg ctctccccga gcgcgggcgt cgcggagacg cgcaggggcg cgccgttcag
      361 ggtcgcgccg cggccgcgct cggccacgaa gcactcgtcg cgactgggat cgtagaggac
      421 gccgaccgcc agctcgccgt ggagctctag cgcgatcgac acgcaataga tcggcagccc
      481 atgggcgtag ttggtggtgc cgtcgagcgg atccacgatc cagcggtgcg gctcggcccc
      541 gctccgcgcc ccgctctcct cggcgaggac gccgtggccg gggaaccggg cgcggatggc
      601 gtcgaggatg atggtctccg cgccccggtc catctcggtg acgagattgg tggggctgcc
      661 cttgtagctg atgtcgtgcc gagcgccgaa gcgggaccgg aggaacgcgc cggcctggag
      721 ggccgcctcc acggcgacgt cgcggaagga atcgctcacg aggatcgagg ataccacggc
      781 gccgcttgac aacccggcgc ccctctcgca gcatggcgcg catgcgcatc gtggtgatgg
      841 gatcgggcgg gaccggcgga ttcttcgggg ccaagctggc gcgggcgggg gaggacgtga
      901 cgttcgtggc gcgcggcgcg cacctggccg cgctgcgcgc ggagggcctg cggatcaagt
      961 ccgccgtcga gggggagtgg agcgtgcggg cgcccgcggt ggagcgcctg gacggtctgc
     1021 cgcccgccga cctcgtgctg ttctgcgtga agtccttcga cacggaggaa gccgccgcgc
     1081 tgatccggcc ggtggtgggc gcggataccg gcgtgctctc gatccagaac ggggtggaca
     1141 acgaggagaa gctcgcgcgc gtgctcggcg ccggccacgt gctggggggc gcggtccggg
     1201 tcttcgctac catcgaggcg ccgggcgtga tcgcgcatac cttcggcggg catctcatct
     1261 tcggggagat ggacgggcgc gagaccgcgc gggcccgcgc cctcctcgcg gcctgtcgga
     1321 aggcgtccat cccggccgag ctcgtgagcg acgtcacccg cgccctctgg gagaagtacg
     1381 tgttcctgac cacgcacgcc gggatgaccg cgctgacccg gtgcccggcc ggggtgctgc
     1441 gggcggtgcc cgaggtgcgg gaggtctacc ggcgcaccgt gaccgagatc gtcgcgatcg
     1501 gcagggccgc cggcgtgaag ctcgacgacg cgatgctcga gcaggggttc aagttcctcg
     1561 acacggtggc gccgaacgcg ttctcgtcgc tccaccacga cctggtccac ggcaagcggc
     1621 tcgagctgga ggccctccac ggccacgcgg tgcggctggc cgagcgccac cacgtgcccg
     1681 cgcccaccgt cttcgcgatc tacgcggcgc tgctcccgta tcgggacggc gcgcccaccg
     1741 cgcccgcgcg ctagcggcgg tgggagcgcg cgggctggcg ccggcgcgcg gctggtctat
     1801 actgactcgg tgtcgtcgct cagacgtctc ctgccgtatc tggaccgcta ccggctccgc
     1861 tacctcgggg gcgcggcctg cctgctcctc gccaccgcgt tctcgctgag catcccgtgg
     1921 acggtgaaga acgcggtgga tgcgctcgag cgcgacggca ccgccgcccg catcggcccc
     1981 tacgtcctcc tgatcctggc cctggccgcc gcgcacggag gcgcgcgcct gggctcgcgc
     2041 ttcgccatgc tgggggccgg ccagtgggtc gagcacgaca tccgcgcgga cgtctacacg
     2101 cggctcctcg ccctgccgcc cgccttctat catcggcatc gcaccggcga tctgatgtcg
     2161 cgcgtctcca acgacatctc caccctgcgg atgctggcgg ggttcgggac cgtgatgctc
     2221 atcggcacca cgctcgcctt cggcggggcg ctgaccgcca tgtggctcat cgacccgtgg
     2281 ctcaccctgt tcgcgctggc gccgttcccg gcgctggtga tcatcgccaa gcgcttccat
     2341 cacgacgcgg aggtgcgcgc cacggcggtg caggagcagc tcggcgcgct ctccgcgaag
     2401 gtccaggaga acctgaccgg catgccggtg gtgcgcgcgt acaccatgga ggcgcgggag
     2461 atcgccgagt tcgagcgcct caacggggag tacctcacgc gcaacatggc gctggcgcgg
     2521 atccagtcgg tgtcgtggcc gctgatgggg ctggtctcgg gtgtgggcgt gctgatcgtg
     2581 atgtggctcg gcggcaaggc ggtggtggac ggtcgcatct cgctcggggc cttcgtggcc
     2641 ttcaacggct acctcaccca gctcgcctgg cccaccatcg cgctgggctg gacgctggcg
     2701 agcatccagc gcggcctggc cgccatgtcg cgggtgaccg aggtcctcga cgagcgggcg
     2761 cacgatggcg tgaaggtgcg tccgcaagac ggtttgggcg ggcaaccggg tggtgactgg
     2821 ggttggcgtc cgcatgttgg tttgggcggt tcttccgacg cccgctccat tgaatgcggg
     2881 ggcccagaga tggcccccgc actcccccaa tctgcggaca accgggagct attgcccggc
     2941 ggaccggttg agttcaaggg gttgaccttc gcctacgacg accggggccc cgtgctgcgc
     3001 gacgtgtcgt tcacggtgcc ggcgggctcg gtggtggcgg tggtggggcc gacggggagt
     3061 ggcaagtcca cgctgggcac gctgctgtgc cgcctctacg agccgccgcg cggcacggtg
     3121 tgggtcggcg gacaggacgt gctcgacctc ccgctcggcc ggctccggcg gtcggtgggc
     3181 tacgtgccgc aggagtcctt tcttttctcg cgtccgctcc gcgacaacgt gcggctggcc
     3241 gacgagggcg cgggcccgga gcgcctggag gcggtggccc ggaccgccgg gctggcggac
     3301 gatctgggcg cgctgccgcg cggctgggac acggtggtcg gggagcgcgg gctcacgctc
     3361 tcgggcggcc agcgccagcg ggtggcgctg gcgcgcgcgc tggtggcgga tccgccgttc
     3421 ctcgtcctgg acgacgtgct ggcctcggtg gacgccgcga aggagtggga gatcacgcgc
     3481 gcgctccggg gagcggccag cgggcgcacc acgctcctga tgacccaccg cctgaaggcc
     3541 gccgccgagg ccgacgcgat cgtggtgctc gacgagggcc gtgtcgtcga gcagggccgc
     3601 cacgccgacc ttctcgcggc cggcggcctc tacgcccgcc tctggcgcgt ccagcagctg
     3661 gaggacgagc tcgcgaatgc ctagctcgcc aatgcgtgat tccgatgcgt gaggccgagc
     3721 aggaagacga ggttctcggc aaggcgtacg acgcgcgcct gatggggcga ctctgggcgt
     3781 tcacgcggcc ccacacccgc cttgtgctgg cgacctgcgc gctcttcccg gcggtggccc
     3841 tgctggagct gtcgcagccc tacctgatca agatcgcgat cgacgaccac atcctccgtc
     3901 gcgactgggc ggggctcggg ggcatcgcgg cgctgttcct gctggtcctt ctcgccctct
     3961 acgcgctgcg ggcggcgcag gcctatctca cccagctcac cggccagcgg gtcatccacg
     4021 acctgcgcgc ggcgctgttc gcgcacctgc agcgccagga cgcgcgcttc tacgaccgga
     4081 gcccggtggg gcggctgatg acccgggtcc tgaacgatgg cgaggcggtc caggagctgt
     4141 tcaccagcgg gatcgtggcg ctgctgggtg acgtggtcgt cctcaccggc gtggtggtga
     4201 tcatgctcgg gatgaactgg aagctggccc tcgtcacgtt cgcgctggtg cccctgctgg
     4261 tgctggccgc cgcctacttc cgcgtgcggg cgcgggacag ctatcgggtg gtccgcacgc
     4321 gtctggcccg gatgaacgcg ttcctgcagg agtcgctcca gggcatgagc gtgatccagc
     4381 tcttcgcgcg cgaggcccgg gagcgggaga cgttcgcgca gctctccggc gcgctgcgcg
     4441 ccggccagtt ccgctcgacg ttctacgacg cctccctcta cgccacggtg gaggcgctgg
     4501 gctccgccgc ggtggccctg ctgctctggt acgggggcgg gcaggtggcg atcggagcgc
     4561 tgaccttcgg cggcctcgtg gccttcctcg agtacaccgg ccgcttcttc ctccccatcc
     4621 gggacctggg cgccaagtac acggtcatgc aggcggccat ggtctcggcc gagcgcatct
     4681 tcggcctgct cgacacccac ccctccatca cctcgcccgt gcccgctgac ggtagctcgg
     4741 gcaggcaggt gcaggacgag gcgccgccga aggcgagccg agcggataca agaaaggaag
     4801 ctccggcggc agcgccccca acccccgccg tggagtttcg taacgtctgg ttcgcgtacg
     4861 aaggcgagca gtgggtgttg cgcgactgct cgttcaccct ggctcccggc gagcgggtgg
     4921 ccctggtcgg gcccaccggc gagggcaaga cgacgatcgc ccggctcatg atccgggcct
     4981 acgacgtcac gcgcggccgc gtcctggtgg agggcgtgga cgtgcgagag tgggatctcc
     5041 agaccctgcg ccggcacgtg gggctggtgc cgcaggaggt attcctcttc tcggggacgg
     5101 tcgaggacaa cctgcgggtg gccggcaacg gcgcggtgga tcggccagag atcgagcgcg
     5161 cgcttgaggc ggcgaacgcg gagcgcttcg tcgcggcgct gccgcgtggg ctccgcgagg
     5221 agctgcgcga gcggggcctc aatctctcgc agggacagcg gcagctgctg gccatcgcgc
     5281 gcgccatcat ctataatcca cgcgttctcg cgctggacga ggcgacttcc agcatcgacc
     5341 ccgaatcgga ggcgcaggtg cgggtcggtc tcgcgcgact gctggaggga cggacgagcc
     5401 tcgtcatcgc gcaccggctg tccacgatcc agcgggcgga tcgcattctc gtgctgcacc
     5461 gcggggagat ccgcgaggcg ggcgggcacg cggagctgct cgcgcagggt ggactctact
     5521 cgcggctgta cgagctccag ttcggcctgg ggcggatgtg aaggcgtttc acgcggcggt
     5581 gtaccggctg gtcgggcgga tcccgagggg acgggtggcg acctacgggc agatcgcggc
     5641 cctgctcgga tatccgcgcg cggcccgcgc ggtggggcag gccatgaagc acgtgcccgc
     5701 ccatctgccg tggcatcggg tggtcaacgc ccagggcggg atcagcctga ggcgcaatgt
     5761 cgggagcatg ctgacgcagc ggctcctgct cgagcaggaa ggcgtgccgg tccgacgggg
     5821 acggatccgg ctccagcagc accggtgggc ggggccaggg cgcacccgtc ggctgtccat
     5881 cgcggcgctc gagcggctct gatcggaggg caccatgcgg ctcggactca acatcggcta
     5941 ctcgcgctcg cagctcggca tcgacctgtc gctggtccag cacgcggatc gcctgggctt
     6001 ccactcggtg tggtcggccg aggcctatgg ctcggacgcg gtctcgccgc tctgctggat
     6061 cgcggcccag accagccgca tcaaggtcgg caccgcgatc atgcagatcc cggcccgcac
     6121 gccgacgctc accgccacca ccgcgatgac gatcgaccag ctctcgggcg gccgcttcat
     6181 tctcggtctg ggcgtgtcgg gcccccaggt cgtggaaggc tggcacggcg tggccttcgg
     6241 caagccgctg gccaagacgc gcgagtacgt ggaaatcgtc aggatggtct ggcggcgcga
     6301 gcggccggtc gagcacaagg gcgagtacta ccagatcccg tacgcggggc ccgacgccac
     6361 cggactcggc aagccgctca aatccatcct gcacggtcgc gcggacatcc cgatctacct
     6421 cgccgcgatc gggccccgga atgtggcgct cgccgccgag atcgccgacg ggctcctgcc
     6481 cgtgttcttc tcgccggagc gcatggcggt gttcaccccg agcctcgagg ccggcttcaa
     6541 gacggccggg ggcgggaaat cgctcgcgag cttcgacatc gcccccacgg tgccgatcgt
     6601 gctgggcccc gacgcggcgg cgtgccgcgc catggtgaag ccgcgactgg cgctctacgt
     6661 gggtggcatg ggcgcccgcg gcaagaactt ctacaacgag ctggtgacgc ggtacggcta
     6721 cgaggcggat gcccggcgga tccaggatct ctacctgggc ggcaagaagg aggaagcggc
     6781 ggccgcggtg cccgacgcgc tggtggacga ggtcgcgctc tgcgggccga aggagcggat
     6841 ccgggagcgg ctgccccgct ggaagagctc cggcatcacc acgctcatct gcggtgcggt
     6901 gcaggcggag gccctcgagc tgatggccga gctggtgctc taggcgtggt gaagccggcg
     6961 gtccagaaag tgcaggcggc cattgccgag cacggcctcg ggcgggaggt gatcgagctc
     7021 gaggtccacg cgcggacctc gcagcaggcc gccgacgcgc tgggcgtcac cgtcggccag
     7081 atcgtgaagt cgctcgtctt cacggtggac gggcgcccgc tcctggtggc tgcctcgggc
     7141 gcgaaccggg tgaacgagcg gcgcctgggc gaggtggcgg gcggccggat ccgccgcgcc
     7201 gatccggaca ccgtgaagca ggccaccggg tacaccatcg gcggggtgcc gccgatcggg
     7261 ctcgcgagtc cgctgccggt gtacgtggac cgcgacctgc ttggctacga gctggtctac
     7321 gcggcggcgg ggctgccgga gtgcgtattc ccgatcgcgc ccgatgagct ggtgcgggcg
     7381 acgggcgggc aggtcgtgga catcaaggaa gccgggagtg attccaaagg agcgcggcca
     7441 tgagactcaa ggacaaggta gccctgatca cgggcgcggg ttcgggcatc gggcaggcga
     7501 cggcggtgct attcgcgcgc gagggcgcgc gggtggcggt cgtggatctg agagaggacg
     7561 cggcgaaggc caccgcggag cagatcgaga agagcggggg ccaggctctc gcgatccggg
     7621 ccgacgtctc gaagtcggcg gacaaccagg ccgccatcca gcaggcggtg gcccgctggg
     7681 gccggctcga catcttctac gccaacgcgg gcgtgcctca gttcccgacg ccggtggaga
     7741 gcgtggacga ggcggtgttc gatcagatca tggcggtgaa cgtgaagggc gtgttcctgg
     7801 cggcgaagca cctgatgccg gtcttccgga agcagaaggg cggcgtgctg ctcatcaccg
     7861 gctcgacctc gggcctgcgg ccgcggcccg gcgtgcagtg ctactcggcc tcgaagggcg
     7921 cggtgcacac cctggccgcg agcctcgcgc tcgagttctc cgcgtttggc gcgcgggtcg
     7981 tcgcgatcgc cccggtggcg accgagacgc cgatgctcgc cacgttcatg ggcaagaagc
     8041 aagtggacga ggagggcatg acccgctacc ggggcaccgt gccgctcggg cggctcaaca
     8101 agccggagga tctggccgcg gcggccctct tcctcgcctc cgacgacgcc gcgatgatca
     8161 cgggcagcgt ctttccggtc gacggcgggc ggtgcatttg aacgtcactc cgctggcggc
     8221 cgagtcgctg ggcgtgcgtt cgatggcgac gtttgtggag tgcggggcga cgcgggtgct
     8281 gatcgatccg ggcgcgatgc tggcgcccaa ccgcttcaac ctggccccgg cggaggagga
     8341 gtgggaggcg ctgcggcgcg ccaacgatcg catctcggcc tacgcgacgc gggcgtccct
     8401 ggtgttcgtg agccactacc acgaggatca cttccgccac gatcccggcc tctacgcggg
     8461 gcggccgctc tgggcgaagg acgcggcgcg gcacatcgag ggccggcagg cccagcgcgc
     8521 ggccacgctc tggcgcgcgc tcaaagggag cgccgagccg ctcgcggcgg agggccggcg
     8581 gctcgagacc gcggacgcgg tgctccaggc ttcgccgccg ctcggtcacg gagcggacgg
     8641 gaccgcgctg ggctacgtgg tggcgctcac catcgaggac cggcgcgcgg gcacgcgctt
     8701 cgtccacgcc tccgacgtac aggggccgct gtcaccggtg gccgccgcct acctggcccg
     8761 gcagcgcccg acgctgctct acctgtccgg cccaccggcg tatctcgagt cgcagatcgg
     8821 cgccgatctc atcgaccggg ggatcgagca cctgctgcgg atcatcgatc agaccggctg
     8881 tcgcgtgatc ttcgatcact acgcgctacg ggacgccgcg tacccggagc gattccgacg
     8941 gctgtgggag accgggcgcg tggtcactgc ggcgggattc ctcgggctgg cggacgcgcc
     9001 gctcgaagcg cgccgggtcg agctctggcc cggccggcgg aagccaccga tgccggtgcg
     9061 cccctcgcgg gcccgcgggg cggcggaagc cggcggcgcg cggccgcccg cgcgcgtcac
     9121 gttcaatacc cggccgggtg tgcggcgggt gagggaggga tgaagcgatg agcgagggca
     9181 cggctcccgg agtgggcgat cccgcgccgg acttcacgct gaagacgatc gggcttcggg
     9241 acgtgagcct caagagcttc cggggccgga acgtggtgct gctcttctac ccgctcgact
     9301 ggacacccgg ctgatcgacc tgcatgcccg ccttcgaccg tcgcatcaag gacttcgagg
     9361 cggcccacac ccaggtcctg ggtatcagct cggacagccc attcagccat gagaactggg
     9421 cgaagtcagt cgggatttcc aactacccgc tgctctccga cattcatcgc accgccggcc
     9481 gcgcctacgg cgtctactgg ccggactgga acgccaacgt gcgggccagc ttcgtgatcg
     9541 acaagcaggg caccatccgc ttcgtggaac ggtacgggcg cggcgagctg cccgatcccg
     9601 acaagatgtt ggcggaggtc cggaagctgc cctaggcggt cgtaggggcc tgggtggtga
     9661 gggcgacgcc cagcagcacg agggcgccgc ccacgatctc cggccactcg accgcctcgt
     9721 gcagcatcgc ccacgccagc gccacgccta ccaccggctg caggttcatg aagatcgcgg
     9781 cccggctcgg gcccaccgcc ttgacgccct cgtaccacca cacgtgggcg atcgccccga
     9841 ggaaggcctg atagagcacg accagccacg cggtctgcga ggtgaagtcg gggcggggga
     9901 agaagggcgc ggccaccagc gtcatgggca gcagcatggc cgagcccagg atgtaggacg
     9961 cggtggtggc gaccgcgggg gagtgcaccg cgagcacctg cttgccgtag atcgtgtagg
    10021 cgctccagcc ggcctgcgag atcatcagga tgaagtcgcc gggcatcaga tcgagatgag
    10081 tgatggcccg ccagctcccc cgcgtgatga ccagcagcac ccccgccatc gaggcgacca
    10141 cccccgccca ctgccggcgt cgcaggcgct ccttcaagta gaaccgtgcg ccgagggcca
    10201 ccatcaccgg ggtggccgcc tgcaggatgg ccccgttggc cgcggtgctg agcgcgatgc
    10261 cgagataagt gccgccggtg gagacgaaga gcccggtgaa gcccaggaac gcgaaggtgc
    10321 gcaggtcgga cgcgcgcagc tcgcggaact cctcccagcc ccgacggaag agcagcaccg
    10381 cgagaaacgc cgaggccagc gtgcagcgta gcgagaccag cacgtagggc gggaagtggg
    10441 cgagggcgag cttggcgaac gcgggatagg agccccagag cgcggagatg agcgtgaggg
    10501 cgaggtacgc ccgcgtttga ccggggtccg gcacccgcct atgatagcgc ggcccccatg
    10561 gcggactaca ttctcgagcg ccgcgtgtgg ctgccgcgtc cccgcgccgt ggtgttcgag
    10621 ttcttcgcgg acccgcgcaa tctcgagaag ctccatcccg cgtgggcgcg gccccgctgg
    10681 ctgaccccgc cgcccgcgcg gctggccgcg ggcaccgtcc tcgaattccg ggccgcgggg
    10741 ctgcccgggc gctggcgcgt gatcgtccgc gagttcgacg cgccctaccg tttcgtggac
    10801 gcgcagctca acggaccgtt cgcccgctgg gagcaccgcc atcggttcac ggaaagcgcg
    10861 ggcggcacct gggtggaaga ccgggtgacc taccgcctgc cgctgggacc gctgggtcgg
    10921 ctcatccacg ccgtgggggc cggtcatcag gtccggcacc tcttcgacta tcgcgaccgc
    10981 cgcctgcgcg acctgttcgg cggcgagtag gcggcgccgc cccctccgag cctagcccgc
    11041 cgcgggcagc tcgatgccga gggcggacag gcgggggcgc acgcgctcgg tcaggagccc
    11101 gatccgggcg aggttgttca ccatcgaggc gtgcatgtcg cgcttgaagt acttccggta
    11161 gaccgcgtag tcgttccggg cggccacgtc gcgccggtag accttcactt cctcgatctg
    11221 ggcgcgcgag aagccgatgt cgcgataggc ctcgaccggg aagaagccgg tcagcacctt
    11281 ctcgagcgcg aggcaggtga agtcctccat ctcctggcgc tccgtgtcgc tcgcctcgcg
    11341 gacgatgtcg ggcagggcca gcatgccgaa gcccatgtgg cgcgactcgt cctgcaggat
    11401 gcgggtgcac acctggcgga ggagcggatc ggcggccgcc tcgcccatca tcttgaagat
    11461 ggcgaccgcg aaggtctcgg ccaccagctg cagcgcgatg gtcttgatgt accagcgcgt
    11521 gtcggtgagg atcgcgtcga agagcacgcg ctcgttctcg ggcatggggt agtggaggcc
    11581 cccgaggcgg gtctggatgt agcgctccag gacctcgttg tggcgcgcct cgtccatgac
    11641 ctgggtggcc tggaagaact tctcgtcggt accctggacg atgtccacca tctggctgca
    11701 ggcgagcatc gcgccctgct cgccgtagac cagcaccgag agccgccacg ccgcgatgcg
    11761 gcggttgagg tccacccggt ccttctcgga gaggcgggcc cagatcgggc tgccgtggat
    11821 gtccaccagc tcgtcggaga gcacgcggcc gtcggtcggc tccggggtgt cccaggcgat
    11881 gtcgcggctg gcgttccact ggtcgcgctt ggccttctcg tagaggctgc gcatgtcctc
    11941 ggcctggacc gagtagtcga actcgaacac ggtctcgatg gtgccgggaa tcacgcggcg
    12001 cgacatcttg ggatcagccg ccgggatatg ctcagccatg ggggcctccg ggaaaactgg
    12061 atagtactgt ttctagactc atcggtttta tcacgggggt cgcgtcg
//
LOCUS       DASUFA010000407         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_191503, whole genome shotgun sequence.
ACCESSION   DASUFA010000407 DASUFA010000000
VERSION     DASUFA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191503"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1886)
                     /gene="leuA"
                     /locus_tag="VFX87_14730"
     CDS             complement(189..1886)
                     /gene="leuA"
                     /locus_tag="VFX87_14730"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX4996236.1"
                     /translation="MARKVGPPAPMPFQHYRPFTPIPLPDRTWPSRVITRAPRWCSVD
                     LRDGNQALIDPMDPERKRRMFDLLVQCGFKEIEVGFPAASQPDFDFIRQLVEEDLIPD
                     DVIIQVLTQARPEQIERTYESLHGAPRAVVHLYNSTSTLQRRVVFGLDRAGIVDIAVR
                     AARLCRELTATIPECDVHYQYSPESFTGTELDFAVEICERVMDVWEPTPAKKVILNLP
                     ATVEMATPNVYADQIEWFLRHVSRRDSIVLSLHPHNDRGTAVAATELAVQAGADRVEG
                     TLFGNGERTGNVDVVTLALNLFSQGVDPKLNFSDIDAARRITEHCNRLPVHPRHPYVG
                     DLVYTAFSGSHQDAIKKGFEALGKNYDIWEVPYLPIDPHHVGRSYEAVIRVNSQSGKG
                     GVAYIMKFEHGFDLPRRLQIEFSSVIQKMVERSGTEITADQIWAAFEATYLKPDQPLG
                     LVHHPTVTTAGEAGETTIEAPLRVGNTERLAKGTGNGPIAAFVDALRRDAGITVSVLD
                     YNEHAMGGGADASAVAYVEVKVGDGRVRWGVGIHANIVSASLRAVVSAVNRELGPDV"
     gene            2074..2820
                     /locus_tag="VFX87_14735"
     CDS             2074..2820
                     /locus_tag="VFX87_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4996237.1"
                     /translation="MIAVHEVTKRFGATPALDAVSLEVRSGTTHVLLGSSGSGKSTLL
                     RLILGLIWPDAGSVRVDGTPVEPATRDALLARMGYVVQDGGLYPHLTAFDNVALAAEA
                     QRWPRARSAARVGELADLTGLDSDMLRRYPRELSGGQRQRVGLMRALVLDPPILLLDE
                     PLGALDPIVRAELQEQLGRLFARLAKTVVLVTHDIREAARLGHTITLLTAGRVVQHGS
                     FADLAERPASPFVTQFLTAQTLPAGARPGA"
     gene            2823..4328
                     /locus_tag="VFX87_14740"
     CDS             2823..4328
                     /locus_tag="VFX87_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4996238.1"
                     /translation="MRVPSRVTLGGVALGALLLSGVGASGAPDGVIRVGSKSFTESYI
                     LAEIAAQVIDQVGEARAERRVGLGGTGLTYRALESGAIDLYAEYTGTLARVLLKDGSL
                     ETPAAIAGRLAPSGLTVSASLGFANTYALAVRADVAERLGLRTIGDLARHPELRAAFS
                     SGFLEREDGWPGLRARYGLALARTEVMEHALTYRAIASGQVDVMDVFSTDGQLERLQL
                     RLLDDDRRFFPDYSAVLLARREMTERYPRSWARLREALEGRLDGAKMARLNAMADLDG
                     RRVSEVAAVFLGGAVPGGPGRHREMLAELAALTVDHLVLVLISVGVAIVLGIPMGIVA
                     ARYRRAGQLELSATAMLQTVPALALLMFMIPLFGIGKGPALVALSLYALLPIARNTYA
                     GLIAIDRTLLEIAGVLRLAPVRRLLRIELPLAAIPIMAGIKTAAVTTVGTATLAAFIG
                     GGGYGALIVRGLALDDTATILAGAAPAALMALAFHALFELADRVLIPRGLR"
     gene            complement(4437..>4855)
                     /locus_tag="VFX87_14745"
     CDS             complement(4437..>4855)
                     /locus_tag="VFX87_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996239.1"
                     /translation="GELPAQLTGVADCASLAVVDDTVFSGLTMRALVSALPEAARRRT
                     HAFCLRGVAESIVAVAALCPVTAGVAAPGRRLHDVSFINASGLVRRVAIRRAGLPPLA
                     FFERPEWLRAWFPEVHAEVLAVCRRLNALLDGSPSG"
BASE COUNT          671 a         1706 c         1764 g          714 t
ORIGIN      
        1 ggtcgccaag cccaagaccg acaagccccg cagcagcggc ggcggctggc gctaagagca
       61 gcatcggagg gggccccttc tgaagtccaa gagggggccc cctccgagcc tcccccagaa
      121 acagcttgcg ccggcgaagc cggcgctcga acggcgacta ggcgcgccgc ccgtatcccg
      181 gtcgcacgtc agacgtcggg gccgagctcg cgattgaccg cgctgaccac cgcacggagc
      241 gaggcggaca cgatgttggc gtggatcccc acgccccagc gtaccctgcc gtctcccacc
      301 ttgacctcga cgtaggccac cgcggaggcg tcggcccccc cgcccatcgc gtgttcgttg
      361 tagtcgagca ccgacaccgt gatgcccgcg tcgcggcgga gggcgtccac gaaggccgcg
      421 atggggccgt tgcccgtccc cttcgcgagc cgctcggtgt tcccgacgcg cagcggcgcc
      481 tcgatggtgg tctcgcccgc ctcccccgcg gtggtgacgg tcggatggtg gaccaggccg
      541 agcggctggt ccggcttgag ataggtcgcc tcgaaggcgg cccagatctg atcggcggtg
      601 atctcggtac cgctccgctc gaccatcttc tggatcacgg acgagaactc gatctgcagc
      661 cggcgcggca ggtcgaagcc gtgctcgaac ttcatgatgt aggccacgcc gcccttgccg
      721 gactggctgt tgacccggat caccgcctcg tagctccggc ccacgtggtg cgggtcgatc
      781 gggaggtacg gcacctccca gatgtcgtag ttcttgccga gcgcttcgaa gcccttcttg
      841 atcgcgtcct ggtgcgagcc cgagaaggcg gtgtagacga ggtcgcccac gtagggatgc
      901 cgcgggtgaa cgggcaaccg gttgcagtgc tcggtgatgc gccgggccgc gtcgatgtcg
      961 gagaagttga gcttcgggtc cacgccctgg ctgaagaggt tgagcgcgag ggtgaccacg
     1021 tccacgttgc cggtgcgctc gccgttgccg aagagcgtgc cctctacccg gtcggccccc
     1081 gcctgtacgg ccagctcggt ggcggccacc gcggtgccgc gatcgttgtg cgggtgcagc
     1141 gagagcacga tcgagtcccg ccggctcacg tggcgcagga accattcgat ctgatcggcg
     1201 tagacgttgg gggtggccat ctccacggtg gcgggcaggt tcaggatcac cttcttggcc
     1261 ggggtcggct cccacacgtc catgacccgc tcgcagatct ccaccgcgaa gtcgagctcg
     1321 gtgccggtga agctctccgg ggagtactgg tagtgcacgt cgcactcggg gatggtggcg
     1381 gtcagctcgc ggcagaggcg ggccgcgcgc accgcgatgt ccacgatgcc ggcccggtcg
     1441 aggccgaaga ccacccggcg ctggagcgtg gaggtcgagt tgtagaggtg caccaccgcc
     1501 cgcggggcgc cgtgcagcga ctcgtaggtg cgctcgatct gctcgggccg cgcctgggtg
     1561 aggacctgga tgatcacgtc gtccgggatc aggtcctctt ccacgagctg gcggatgaag
     1621 tcgaagtcgg gctgggaggc ggccgggaag cccacttcga tctccttgaa gccgcactgc
     1681 accagcaggt cgaacatccg ccgcttgcgc tccgggtcca tcgggtcgat cagggcctgg
     1741 ttgccgtcac gcaggtccac gctgcaccag cgcggcgcgc gggtgatcac ccgggacggc
     1801 cacgtgcgat ccggcagcgg gatcggggtg aacggacggt agtgctggaa cggcatcgga
     1861 gccggtggac ccaccttgcg cgccattgaa cgcctccctc tcgatagaaa caaaaaagcc
     1921 tccccgggac atgggagagg cttttggcgc gcgccgcgaa aggcgcgcgc ctaactcagt
     1981 agcagggcct tgaagagcgc agccacctgc gtcatgtgcc gagaattgtg cccagaccgg
     2041 gggcgattgt caagcttccg gtagaatgcg ggggtgatcg cggtccacga ggtcacgaag
     2101 cgcttcggcg ccaccccggc cctcgacgcg gtgtccctgg aggtgcgctc cggcaccacc
     2161 cacgtgctcc tggggtcgag cggctcgggc aagtccaccc tgctgcggct catcctgggc
     2221 ctgatctggc cggacgcggg ctccgttcgg gtggacggca ccccggtgga gcccgccacg
     2281 cgcgacgccc tcctggcccg gatgggctac gtggtacagg acggcgggct ctatccccac
     2341 ctcaccgcgt tcgacaacgt ggcgctggcc gcggaggcgc agcgctggcc gcgcgcccgg
     2401 agcgcggccc gggtcggcga gctggcagac ttgaccgggc tcgacagcga catgctgcgg
     2461 cggtatccgc gcgagctcag cggcggccag cgtcagcggg tggggctgat gcgcgccctc
     2521 gtgctggacc cgcccatcct gctgctcgac gagccgctcg gcgcgctcga tcccatcgtc
     2581 cgcgcggagc tgcaggagca gctcgggcgg ctcttcgcgc gcctggccaa gacggtggtg
     2641 ctggtcaccc acgacatccg cgaagccgcg cgcctcggtc acaccatcac cctgctcacc
     2701 gccgggcgcg tggtccagca cggcagcttc gcggacctgg ccgagcggcc ggccagcccg
     2761 ttcgtcaccc agttcctcac cgcccagacc ctgcccgcgg gcgcgcggcc gggcgcctga
     2821 gcatgcgcgt gccctcgcgc gtgacgctcg gcggcgtggc gctcggcgcg ctcctgctca
     2881 gtggcgtggg cgcgtccggg gcgcccgacg gcgtcatccg cgtgggctcg aagtccttca
     2941 ccgagagcta catcctggcc gagatcgcgg cccaggtcat tgatcaggtc ggcgaggcgc
     3001 gggccgagcg gcgcgtgggt ctgggcggca ccggcctgac ttaccgggcg ctcgaatccg
     3061 gcgcgatcga cctctatgcg gaatacaccg gcacgctcgc gcgggtcctc ctgaaggacg
     3121 gctcgctcga gacgcccgcg gccattgccg ggcgcctggc gccgagcggg ctcacggtca
     3181 gcgcctcgct cggcttcgcc aatacctatg ccctcgcggt ccgggccgac gtggcggagc
     3241 gcctcgggct ccggaccatc ggcgatctgg cccggcaccc ggagctgcgc gctgcgttca
     3301 gctcgggctt tctcgagcgg gaggacggct ggcccggcct gcgcgcgcgc tacggcttgg
     3361 cgctggcccg gacggaggtg atggagcacg cgctcaccta ccgcgcgatc gcgagcggcc
     3421 aggtggacgt gatggacgtg ttctccaccg acggccagct cgagcggctg cagctgcggc
     3481 tcctcgacga cgaccggcgc ttcttccccg actactccgc ggtgctgctg gcgcgccgcg
     3541 agatgaccga gcgctatccg cgctcctggg cgcggctgcg ggaggcgctg gagggccggc
     3601 tcgacggcgc gaagatggca cggctgaacg cgatggcgga tctggacggc cggcgcgtct
     3661 cggaggtggc cgcggtcttc ctgggcggcg ccgttccggg gggcccgggg cgccaccggg
     3721 agatgctggc cgagctcgcc gccctcaccg tggatcatct cgtgctcgtg ctgatctcgg
     3781 tgggcgtggc gatcgtgctc ggcatcccca tgggcatcgt cgccgcgcgc taccggcggg
     3841 ccggccagct cgagctctcc gcgaccgcga tgctgcagac ggtgcccgcg ctggccctgc
     3901 tgatgttcat gatcccgctc ttcggcatcg gcaagggccc cgcgctggtc gccctctcgc
     3961 tctacgcgct gctgccgatc gcgcggaaca cctacgcggg cctgatcgcc atcgaccgca
     4021 ccctgctcga gatcgctggg gtgctgcgcc tggctccggt gcgccggctg ctgcggatcg
     4081 agctgccgct ggccgcgatc ccgatcatgg ccgggatcaa gaccgcggcg gtgaccaccg
     4141 tgggcacggc caccctggcc gccttcatcg gcggcggcgg ctacggcgcc ctgatcgtcc
     4201 gcggccttgc gctcgacgac accgccacca tcctggccgg ggccgcgccc gcggcgctca
     4261 tggccttggc ctttcacgcg ctgttcgagc tggccgaccg cgtgctgatc ccccgcgggc
     4321 tgcggtgaag aagcgggaag ggggtaacgg ttgactgcgg accgggatga ctgcggccga
     4381 agctttgtag gtgagccgaa ggcgagccga gcgaataaga aagagaagct acggtgtcag
     4441 ccggaagggg acccgtctag caacgcgttg agtcgccggc aaaccgcgag aacttcggcg
     4501 tgaacctccg gaaaccacgc gcggagccac tcgggccgct cgaagaaggc gagcggcggc
     4561 agcccggcgc gccggatggc cacgcgccgc acgagcccgc tcgcgttgat gaagctcacg
     4621 tcgtggagcc ggcgcccggg ggccgcgacg cccgcggtga cggggcagag cgccgccacc
     4681 gccacgatgg actcggcgac gccgcgcagg cagaaggcgt gggtgcgccg gcgcgcggcc
     4741 tcgggcagcg ccgagaccaa ggcccgcatc gtgaggccgg agaagacggt gtcgtccacc
     4801 accgcgagcg acgcgcagtc cgccaccccg gtgagctggg ccgggagctc gcccg
//
LOCUS       DASUFA010000408         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_788716, whole genome shotgun sequence.
ACCESSION   DASUFA010000408 DASUFA010000000
VERSION     DASUFA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788716"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFX87_14750"
     CDS             complement(<1..723)
                     /locus_tag="VFX87_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4996240.1"
                     /translation="MRDEQSIETEATELLRTLIRNACVNDGTAASGQETRSCAALEDY
                     FAGAGVACERYTSEPGRESLISRIEGSDPKAPRLLLMGHTDVVPVSPDGWKRDPFGGE
                     LVDGVVWGRGAIDMLNLTSTMAVATRRLARSGFRPRGTLIYLAVADEEAGGNLGAGHL
                     VERRPDAVKADYVITESGGVPIPTGKGHMLGVTVGEKGANWRRLTVAGTPGHGSRPFR
                     TDNALVTAAEVVRRIAAYQPKAR"
     gene            complement(748..1641)
                     /gene="nudC"
                     /locus_tag="VFX87_14755"
     CDS             complement(748..1641)
                     /gene="nudC"
                     /locus_tag="VFX87_14755"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX4996241.1"
                     /translation="MTTTLTPLSVYLPFNMDCLRGEFMPAKRGGGGPPTGTGRWLIVQ
                     DQKLLVVPDGEGLRLPAGAAPEAPPRGLDGLGPRHWLGTWAGEPCWVAALPKGAPVPE
                     GLRPETLVPMQGTRLPDDLLTLGGMAMQLLYWESTSGHCPRCGAPAGRLEGEWGKRCG
                     ACQYEHYPHLHPAVIVLVRDGDRVLLARKAIWAPGRYALVAGFVDNGESLEGCVAREV
                     KEEVGVDVKDIQYVGSQNWPFPSQIMIGFIASYAGGEITIDRDELEDARWFPRTEMPV
                     GPARHSIAGWIIRNFSGNVGM"
     gene            1711..2211
                     /locus_tag="VFX87_14760"
     CDS             1711..2211
                     /locus_tag="VFX87_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX4996242.1"
                     /translation="MPELLSALAASVAARLLARKETIAVSESSTGGLISAALLSIPGA
                     SAYFVGGGVIYTQTARRGLLQLPDDAVRGIRSSTEAAARLNAKTVRERLGTTWALAET
                     GAAGPTGNRYGDAAGHSCFAVAGAVEAARTLETGRSDREANMWAFTRAALELLDTCLQ
                     QAGEKP"
     gene            2208..3251
                     /locus_tag="VFX87_14765"
     CDS             2208..3251
                     /locus_tag="VFX87_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4996243.1"
                     /translation="MTDAKIRVGFIGAGANTRKHHIPKLKAQPGVELVAVANRSKESG
                     EKVAREFGIGRVQASWQEIVNAPDIDAVCIGTWPYMHCEMTTAALGAGKHVLCEARMA
                     MNATEGRRMLEASRKAPSLIAQLVPAPHTLELDATVQALLAEGFLGEVLAVELQSSGG
                     RFVEPDAPLHWRHDVALSGHNILNMGIWYEAMMRWLGPAQRVTSMTKVAVPRRKDATG
                     ATHDVKVPDHVDILAQLRRGAVAHMRFSSVTALAPASEAWIYGSEGTLRLEADALRLT
                     GARRGEPALREIPVPPKTRIGWRVEEEFVNAIRGREKIARTTFEDGVRYMEFTDAVTR
                     SAATGQTVEVKDL"
     gene            complement(3342..3488)
                     /locus_tag="VFX87_14770"
     CDS             complement(3342..3488)
                     /locus_tag="VFX87_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996244.1"
                     /translation="MNDLVVQKAVVIPVTWRTVLHAAANNLACIDLNSWDSIFGRIAY
                     WYRA"
BASE COUNT          497 a         1275 c         1248 g          494 t
ORIGIN      
        1 gcgcgccttc ggctggtagg ccgcgatgcg ccgcacgact tccgcggcgg tcaccagggc
       61 gttgtcggtg cggaacgggc gcgagccgtg gcccggcgtg cccgccaccg tgagccgccg
      121 ccagttggcg cccttctcgc ccaccgtcac gcccagcatg tggcccttgc cggtcgggat
      181 cggcaccccg ccgctctcgg tgatgacgta gtccgccttc accgcgtcgg gccgccgctc
      241 gacgagatgt cccgcgccga gattgccgcc ggcctcctcg tccgccaccg ccaggtagat
      301 cagcgtgccg cgcgggcgga agccgctcct ggccaggcgc cgtgtcgcta ccgccatcgt
      361 gctggtgagg ttcagcatgt cgatggcgcc gcggccccag accacgccgt ccaccagctc
      421 gccgccgaag ggatcgcgct tccagccgtc cgggctgacc ggcaccacgt cggtgtggcc
      481 catcaggagc aggcgcgggg ccttggggtc cgagccctcg atgcgggaga tgaggctctc
      541 gcgcccgggt tcggaggtgt agcgctcgca cgcgaccccg gcccccgcga agtagtcctc
      601 cagcgccgcg cagctccgcg tctcctggcc ggaggccgcg gtcccgtcgt tgacgcacgc
      661 gttgcggatc agggtgcgca gcagctcggt cgcctcggtc tcaatgctct gctcgtctcg
      721 catgggttcc tcgattgggg tcaggtctta cattccgaca ttgccagaga agttccggat
      781 gatccagccc gcgatggagt gacgggccgg gcccaccggc atctcggtgc gcggaaacca
      841 ccgcgcgtcc tcgagttcgt cgcggtcgat ggtgatctcg ccgcccgcgt agctcgcgat
      901 gaagccgatc atgatctggc tcgggaacgg ccagttctgg ctgcccacgt actggatgtc
      961 cttgacgtcc accccgacct cttccttcac ctcgcgcgcc acgcagccct cgagcgactc
     1021 gccgttgtcc acgaagcccg cgaccagcgc gtagcggccc ggcgcccaga tcgccttgcg
     1081 cgcgagcagc acgcggtcgc cgtcgcgcac cagcacgatg accgcgggat ggagatgcgg
     1141 gtagtgctcg tactggcagg ccccgcagcg cttgccccac tcgccctcga ggcgtccggc
     1201 gggtgcgccg cagcgcgggc agtggccgct cgtggactcc cagtacagga gctgcatcgc
     1261 catgccgccc agggtcagca gatcgtcggg cagccgcgtg ccctgcatgg gcacgagcgt
     1321 ctccggccgg aggccctccg gcacgggtgc ccccttgggc agcgcggcga cccagcaggg
     1381 ctcgccggcc caggtgccga gccagtggcg cgggcccaag ccgtcgaggc cgcgcggcgg
     1441 ggcttccggc gcggcgcccg cgggcagccg gaggccttcg ccgtcgggca ccaccaggag
     1501 cttctggtcc tggacgatca gccagcggcc ggtgccggtg ggcggcccgc cgcccccgcg
     1561 cttggcgggc atgaactcgc cgcggaggca gtccatgttg aagggcaggt agacggacag
     1621 cggggtcagc gtcgtggtca tcggtgcggc cgagtctatc acggagaaaa cccggttgcg
     1681 ctccggcccc gaccggggta ccactacccc gtgcccgagc tcctctccgc cctcgccgcc
     1741 tccgtcgccg cgcgtctcct cgcgcgcaag gagacgatcg cggtctccga gtcctcgacg
     1801 ggcggcctga tctcggccgc gctgctctcg atcccgggcg cctccgcgta cttcgtgggc
     1861 ggcggcgtga tctacacgca gaccgcgcgc cgcggcctgc tccagctccc cgacgacgcg
     1921 gtgcgcggca ttcgctccag caccgaggcc gccgcgcgcc tcaacgcgaa gacggtgcgc
     1981 gagcggctcg gcacgacctg ggcgctcgcc gagacgggcg cggccggccc caccggcaat
     2041 cgctatggcg acgcggccgg gcactcgtgc ttcgcggtcg cgggcgcggt ggaggcggcc
     2101 cgcacgctcg agacgggccg gagcgaccgc gaagccaaca tgtgggcctt cacccgggcc
     2161 gcgctcgagc tgctcgacac ctgcctccaa caagcgggag agaaaccatg acggatgcca
     2221 agatccgcgt cggcttcatc ggcgccggtg ccaacacgcg caagcatcac atccccaagt
     2281 tgaaggccca gcccggcgtc gagctggtcg cggtcgccaa ccggtcgaag gaatcgggcg
     2341 agaaggtcgc gcgcgagttc ggcatcggcc gggtgcaagc gagctggcag gagatcgtca
     2401 acgcgcccga catcgacgcg gtgtgcatcg gcacctggcc ctacatgcac tgcgagatga
     2461 cgaccgccgc gctcggggcg ggcaagcacg tgctctgcga ggcgcgcatg gccatgaacg
     2521 ccaccgaggg ccggcgcatg ctggaggcct cgcggaaggc gccgagcctg atcgcccagc
     2581 tcgtgcccgc gccccacacg ctcgagctgg acgccaccgt ccaggcgctg ctcgccgagg
     2641 gcttcctggg ggaggtgctg gcggtcgagc tgcagtcctc ggggggccgc ttcgtggagc
     2701 cggacgcccc gctgcactgg cggcacgacg tcgcgctgag cggccacaac atcctcaaca
     2761 tggggatctg gtacgaggcc atgatgcgct ggctcggccc cgcgcagcgg gtgacctcga
     2821 tgaccaaggt cgcggtgccc cggcgtaagg acgcgacggg cgcgacccac gacgtgaagg
     2881 tcccggatca cgtggacatc ctggcccagc ttcggcgcgg cgcggtcgcg cacatgcgct
     2941 tcagctcggt gaccgcgctg gcccccgcct ctgaggcgtg gatctacggg agcgagggga
     3001 cgctgcggct cgaggcggac gccctccggc tcaccggcgc ccggcgcggg gagccggcgc
     3061 tgcgcgagat cccggtgccg cccaagacgc gcatcggctg gcgggtggag gaggagttcg
     3121 tcaacgccat ccggggccgc gagaagatcg cgcgtaccac cttcgaggac ggcgtgcgtt
     3181 acatggagtt cacggacgcg gtgacccgca gcgccgccac cggccagacc gtcgaggtga
     3241 aagacctcta agtccggagt ctggaggcct cctaaccccg tccccctctc ccctcagggg
     3301 agagggcagg gtgagggatg gtcggcgggt gagaggtgat tctaggcccg gtaccagtac
     3361 gcgatccggc cgaagatcga gtcccacgaa ttcaggtcga tgcaggccag gttgttggcg
     3421 gcggcgtgaa gcacggtgcg ccacgtcacc ggaatcacca ccgccttctg caccacgagg
     3481 tcgttcatct agatgaacag cgcggcccgc ttga
//
LOCUS       DASUFA010000409         8836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1359791, whole genome shotgun sequence.
ACCESSION   DASUFA010000409 DASUFA010000000
VERSION     DASUFA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359791"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFX87_14775"
     CDS             complement(<1..498)
                     /locus_tag="VFX87_14775"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEX4996245.1"
                     /translation="MAEHTPVADPKMERRVIPGTIETVFEFDYSVQAEDMRSLYEKAK
                     RDQWNASRDIAWDTPEPSDGRVLSDELVDIHGSPIWARLSEKDRVDLNRRIAAWRLWV
                     LVYGEQGALLACSQMVDIVQGTDEKFFQATQVMDEARHNEVLERYIQTRLGGLHYPMP
                     ENERVL"
     gene            598..1260
                     /locus_tag="VFX87_14780"
     CDS             598..1260
                     /locus_tag="VFX87_14780"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4996246.1"
                     /translation="MNARRKAVRAQILRAAADLFRERGYQASTVEHIAARLGMSKASL
                     YTHFRAKEEMLAAISRETIEAFTRDLNLVLGSRLDPAEKLRRMVRQHVRFVIANRSFL
                     TVFFGEEANLPPRFARSLAAQKDRYDKGVERIVLEGVRAGVFRDVPPRLVVFGVLGMV
                     NWVHKWYNPAGRWGAEEVSTAFLSLIEGGLLRQKRRGPGLSGRLRRLERELHEVARQL
                     DA"
     gene            1253..2110
                     /locus_tag="VFX87_14785"
     CDS             1253..2110
                     /locus_tag="VFX87_14785"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX4996247.1"
                     /translation="MPDLLGAHMSIAGGLARALERGLEVGCSVVQIFLKNQRQWKARP
                     YTDSEVREFRAAWKTSRIRVVFAHANYLINLATPVAAEWRRAVDTFHDELERAEALGL
                     PFVVIHPGSHREAGLEAGIQRIARAVDLLHERTRGYRVRVVLENTAGGGSTIGRSFEE
                     LAAIVAAVREPARVGICLDTCHLFAAGYDIRTADGYRSAMARCGRLLGTRRVRAFHLN
                     DARQPLGSGLDRHEKIGHGHLGVETFRRLMNDRRFARVPMALETPKDPEPRADREALA
                     LLRRLRKRS"
     gene            complement(2121..2768)
                     /gene="nth"
                     /locus_tag="VFX87_14790"
     CDS             complement(2121..2768)
                     /gene="nth"
                     /locus_tag="VFX87_14790"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX4996248.1"
                     /translation="MSIGHTIRRLRREAPGWRTTALAEVAAQWHDPFRVLVACLLSLR
                     TKDQTTGPAAARLFALADTPEGIRRLTPRAIERAIYPVGFYRTKARVLREISRDLLAR
                     FGGAVPDDIDALLTLKGVGRKTANLVVTQGFNKPGICVDVHVHRISNRWGFVKTRTPE
                     ETELALRRSLPRRYWIGYNDLLVSFGQNICLPVSPRCSECPIRAGCPRRGVVHSR"
     gene            complement(2765..3499)
                     /locus_tag="VFX87_14795"
     CDS             complement(2765..3499)
                     /locus_tag="VFX87_14795"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEX4996249.1"
                     /translation="MRTAAAIVMLLSAAGAASAQSPLAAEVRGLAGRYHENPGRIDAL
                     RPLVAQAAQADPSVDNLLALAQIAFLYGDVRALRPEDKLDAYEQGLQAARRAAEIAPR
                     NARAHFWYATNAGRWGQTKGVLRSLFLLPEVKRGMETALELDPQFPPAYVLAGTVYYE
                     VPGLVGGDLERSERLFRRGLEVDGHFTGLRVGLARTLIKRGRVAEARRELQAVLDEKV
                     PSNPADWTLKDSPEARRLLAERPARS"
     gene            3589..4050
                     /locus_tag="VFX87_14800"
     CDS             3589..4050
                     /locus_tag="VFX87_14800"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX4996250.1"
                     /translation="MRILPAAAILVWLGIMAFFAGVVAPAAFSALDREAAGRFVSAVF
                     PRYYVVGAVLGALALAGLGLRFALGPRRPWDGLPLLLVAVMLATTLYAWLVVLPAAHA
                     AREALRQSVPVPGAVSTEALAFARLHRLSGLLNGAAMVAGVLALAAVGLRR"
     gene            4047..4424
                     /locus_tag="VFX87_14805"
     CDS             4047..4424
                     /locus_tag="VFX87_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4996251.1"
                     /translation="MSRIFEPLGFDHVVLHVRDQAASERFYVDVLGCTLDHVNKPISL
                     VQLRFGEHLIDLLPGERTEGGGLDHFCLSIRCGDLTRVRDELRARGVAVDGDVVQRRG
                     AFGSGPSLYIQDPDGYRIELKPR"
     gene            complement(4411..4872)
                     /locus_tag="VFX87_14810"
     CDS             complement(4411..4872)
                     /locus_tag="VFX87_14810"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX4996252.1"
                     /translation="MAPAATVPAASQVEQRGLPIVAELARVASGPGAPRVKLEGAMEI
                     LFGAYGESDPDFSGLFLHGWMRARHDKQFRLTLAWQREQIRLSLEDILVEGVRAGAFR
                     AGMDAAAVAAVIVGAAEGCLLQAATDGGAVTAEQIVRTLLTLAVGGTVSGA"
     gene            complement(5001..5375)
                     /locus_tag="VFX87_14815"
     CDS             complement(5001..5375)
                     /locus_tag="VFX87_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEX4996253.1"
                     /translation="MMLRALLAAAGILLALAPAAQAQTYTPQSSSRLAVSFTTERVGS
                     GRVLIYGEVRNGTNASCERVVVSVEGIDESGRVVSRGRAYVFGVVPSRGSSPFEVRMS
                     SPGSERRFRVEIESFQFVSSGN"
     gene            complement(5372..6604)
                     /locus_tag="VFX87_14820"
     CDS             complement(5372..6604)
                     /locus_tag="VFX87_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996254.1"
                     /translation="MNVLASARIAVRALRVNKLRSALTMLGIVIGVGAVITMVAVGAG
                     AQARVAEQIQSLGANLIIIWPGTVNLGGVRLGAGAQATLSEDDALAIERDVALVEVVA
                     PSVRGGAQVVYSNLNWSTTILGVTPEYLPARDWELSAGRSLTREEVEGAFKVALVGQT
                     VAQNLFGEEDPLGQVIRIKRVPFTIIGVMDRKGQTTWGQDQDDQILIPLSTAKTKVVG
                     GNQAKGRAVGAISIKVRDAGLMKEAEFEIRELLRQRHRLQPHQEDDFNLRNLAEVLQS
                     QEAASRVLTVLLAAIASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARGKDILTQ
                     FLVEAVTLSLIGGVIGVGAGLLGSYAIAYFAEWRTLLQFDAILLAFGFSAAVGIFFGF
                     YPARKAAGLNPIDALRYE"
     gene            complement(6601..7380)
                     /locus_tag="VFX87_14825"
     CDS             complement(6601..7380)
                     /locus_tag="VFX87_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996255.1"
                     /translation="MPEPLIATEDLVKDYHLGTRRIHALRKVSISIQPGEFVAVMGPS
                     GSGKSTFMNLLGCLDTPTSGQYRLDGLDVSRMGRDRLARVRNEKIGFVFQTFNLLPRT
                     SALENVELPLLYSAIQGRDRQARARAKLAAVGLSEREHHHPAQLSGGQQQRVAIARAL
                     INDPVLILADEPTGALDSHTSVEIMALFQDLNREGITVVVVTHEADIARYAGRALGFR
                     DGRLIRDEEVRDRLVAREQLASLPRGEEQEEEDVEAEVASP"
     gene            complement(7382..8767)
                     /locus_tag="VFX87_14830"
     CDS             complement(7382..8767)
                     /locus_tag="VFX87_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4996256.1"
                     /translation="MKRRLLWIVGIVLGAGVISGGYLYTQGIGARATFRTATVSRGPL
                     TSAVSATGTLNAVITVLVGSQISGQVKELFADFNSQVKKDQVIARIDPETYEAKVSQA
                     KAQVDATQASVLNQQAVVEKTRADLENARAAFAAARAQTAKAQVAVVDSKRTLGRNQD
                     LRKRGLIAQADEDTAQAAYDSAVAQVEASKAQEDAQVSTVRAAEAQLRVSEAMLKNAA
                     AQVVQSQAALRQAQVDLERTYIRAPVDGTVVGRSVDVGQTVAASLQAPTLFTIAQDLR
                     NMQVDTNVDEADVGRVKVGQTATFTVDSFPGRTVTGEVVQIRKAPLVVQNVVTYDVVV
                     TAKNPEQRLLPGMTANVRIIIDQKEGVLQVPNAALRFRPAGVEAPERERPARPGGGGG
                     RGGAAPVGRVWVVGSDGKPAALALQLGISDGTYTEVAKGEIKEGQQVIVGVVAPGDRP
                     QQSQGAPRLRL"
BASE COUNT         1186 a         3144 c         3185 g         1321 t
ORIGIN      
        1 gagcacgcgc tcgttctcgg gcatggggta gtggaggccc ccgaggcgcg tctggatgta
       61 gcgctccagg acctcgttgt ggcgggcctc gtccatgacc tgggtggcct ggaagaactt
      121 ctcgtcggta ccctggacga tgtccaccat ctggctgcag gcgagcaacg cgccctgctc
      181 gccgtagacc agcacccaga gccgccacgc cgcgatgcgg cggttgaggt ccacccggtc
      241 cttctcggag aggcgggccc agatcgggct gccgtggatg tccaccagct cgtcggagag
      301 cacgcggccg tccgacggct caggagtgtc ccatgcaata tcgcggctgg cgttccactg
      361 gtcgcgcttg gccttctcgt agaggctgcg catgtcttca gcctggaccg agtagtcgaa
      421 ctcgaacacg gtctcgatgg tgccgggaat cacgcggcgt tccatcttgg gatcagccac
      481 cggggtatgc tcagccatcg ggccggcctc ctgtgtatct cggaaactcg agagtactat
      541 ttctagactc attggtttta tcacgggggt cgccacgggg tcaagtagac tgggccggtg
      601 aacgccaggc gcaaggcggt ccgggcccag atcctccgcg ccgccgcgga tctcttccgg
      661 gagcgcggct accaggcctc taccgtcgag cacatcgcgg cgcgcctcgg gatgtcgaag
      721 gcctcgctct acacccactt ccgggccaag gaggagatgc tcgccgccat ctcgcgggag
      781 accatcgagg ccttcacccg cgacctcaac ctggtgctgg gctcccggct ggaccccgcg
      841 gagaagctgc gccgcatggt gcggcagcac gtgcggttcg tcatcgccaa ccgttccttc
      901 ctgaccgtct tcttcggcga ggaggcgaac ctgcccccgc ggttcgcgcg ctccctggcc
      961 gcccagaagg accgctacga caagggggtc gagcgcatcg tgctggaagg cgtgcgcgcg
     1021 ggcgtcttcc gggacgtgcc gccccggctg gtggtgttcg gggtcctcgg gatggtcaac
     1081 tgggtccaca agtggtacaa cccggccggc cggtggggcg ccgaggaggt ctccaccgcc
     1141 ttcctctccc tgatcgaggg cggactcctg cgccagaagc gccgcgggcc tgggctgagc
     1201 gggcggctgc gccggctcga gcgcgagctc cacgaggtcg cccgccagct cgatgcctga
     1261 cctcctgggc gcgcacatgt ccatcgcggg cggcctcgcc cgcgcgctgg agcggggcct
     1321 ggaggtgggc tgctcggtgg tgcagatctt cctcaagaac cagcgccagt ggaaggcccg
     1381 gccctacacc gacagcgagg tccgcgagtt ccgcgccgcc tggaagacgt cccggatccg
     1441 ggtggtcttc gcccacgcca actacctcat caacctggcc acgccggtgg ccgcggagtg
     1501 gcggcgcgcg gtggacacct tccacgatga gctggagcgc gcggaggcgc tcggcctgcc
     1561 gttcgtggtg attcacccgg gctctcatcg cgaggcggga ctggaggccg gcatccagcg
     1621 gatcgcccgc gcggtggacc tgctgcacga gcggacgcgc ggctaccggg tgcgcgtggt
     1681 gctggagaac accgcgggcg gcggctccac catcggccgg tcgttcgagg agctggccgc
     1741 gatcgtggcc gcggtgcgcg agcccgcgcg cgtgggcatc tgcctcgaca cctgtcatct
     1801 cttcgcggcc ggctacgaca tccgcaccgc cgacggctac cgcagcgcga tggcgcgctg
     1861 cgggcggctg ctcggcacac gacgcgtgcg cgcgtttcac ctcaacgacg cccgccagcc
     1921 cctcggctcc ggcctcgacc ggcacgagaa gatcggccac ggccacctgg gcgtggagac
     1981 gttccgtcgg ctgatgaacg accggcgctt cgcgcgggtg cccatggcgc tcgagacgcc
     2041 gaaggacccg gagccgcgcg cggaccgcga agccctcgcg ctcctgcgcc gcctgcgcaa
     2101 acggtcctga gcccggccgc ctagcgcgag tggacgacgc cgcggcgtgg gcagcccgcg
     2161 cggatcgggc actccgagca gcggggcgag acgggcaggc agatgttctg tccgaaggac
     2221 acgagcaggt cgttgtagcc gatccagtag cggcggggca ggctgcgccg gagcgccagc
     2281 tcggtctctt ccggcgtgcg cgtcttgacg aagccccagc ggttcgagat ccggtgcacg
     2341 tgcacgtcca cgcagatgcc cggcttgttg aatccctggg tcaccaccag gttcgcggtc
     2401 ttgcggccca cgcccttcag ggtcagcagc gcgtcgatgt cgtcgggcac ggccccgccg
     2461 aagcgcgcca gcagatcccg gctgatctcg cggagcacgc gcgccttcgt ccggtagaag
     2521 ccgaccgggt agatggcccg ctcgatcgcg cgcggggtga ggcggcggat gccctccggc
     2581 gtgtcggcca gcgcgaacag ccgcgccgcc gccggtccgg tggtctggtc cttggtgcgc
     2641 agggagagca ggcaggccac cagcacgcgg aagggatcgt gccactgcgc cgccacctcg
     2701 gccagcgcgg tggtccgcca ccccggcgcc tcgcgccgca gccgccggat ggtgtggccg
     2761 atgctcatga gcgggccggg cgctcggcca ggagccggcg ggcctcgggc gagtccttca
     2821 gcgtccagtc cgccgggttg gacgggactt tctcgtcgag cacggcctgc agctcgcgcc
     2881 gcgcttccgc gacgcgcccg cgcttgatca gggtgcgggc gaggccgacc cgcaggcccg
     2941 tgaagtggcc gtccacctcg aggcccctcc ggaagagccg ctcggacctc tccagatcgc
     3001 cgccgacgag cccgggcact tcgtaataga cggtgccggc gagcacgtag gcgggcggga
     3061 actgcgggtc cagctcgagc gcggtctcca tgccgcgctt gacctcgggc aggaggaaga
     3121 gcgagcgcag cacgcccttg gtctgccccc atcgacccgc gttggtggcg taccagaaat
     3181 gggcccgggc gttgcggggc gcgatctcgg cggcccgccg ggccgcctgg aggccctgct
     3241 cgtaggcgtc gagcttgtcc tccggccgaa gggcccgcac gtcgccgtag aggaaggcga
     3301 tctgggccag ggccagcagg ttgtccacgc tcgggtcggc ctgcgcggcc tgcgccacca
     3361 gagggcggag cgcgtcgatg cgcccggggt tctcgtggta gcggccggcg aggcctcgga
     3421 cctcggcggc gagcggggac tgagcggacg cggcgcccgc cgcgctcagg agcatgacga
     3481 tcgccgcggc cgtgcgcatc gcgcccgatg ctaggggccc tgcgcgcgac tgtcaaccgc
     3541 ggcggcccgc cttgcgatgc cgccacgccg ccggtatggt agggcgcgat gagaatcctg
     3601 cccgcggcgg cgatcctggt ctggctcggc atcatggcct tcttcgccgg ggtcgtggcg
     3661 cccgccgcct tctccgcgct cgaccgcgag gcggccggcc gcttcgtgag cgcggtgttc
     3721 ccgcgctact acgtggtggg cgcggtgctg ggcgcgctcg cgctggccgg gctcggcctc
     3781 cggttcgccc tcggcccccg ccggccctgg gacgggctgc cgcttctcct ggtcgcggtg
     3841 atgctggcca cgacactcta tgcctggctc gtcgtgctgc ccgccgcgca cgcggcccgc
     3901 gaggcgctgc ggcagagcgt gcccgtgccg ggcgcggtct ccacggaagc gctggctttc
     3961 gcgcgcctgc accggctctc cggcctgctg aacggtgccg cgatggtcgc gggcgtgctc
     4021 gccctagccg cggtggggct caggcgatga gccggatctt cgagccgctc ggtttcgatc
     4081 acgtggtgct ccacgtccgc gaccaggccg cgtcggagcg cttctacgtg gacgtgctcg
     4141 gctgcacgct cgaccacgtg aacaagccga tctctctggt acagctccgc ttcggcgagc
     4201 acctgatcga cctgctgccc ggcgagcgca cggagggcgg cggcctcgac cacttctgcc
     4261 tctcgatccg ctgcggcgac ctcacgcgcg tccgcgacga gctccgggcg cgcggggtgg
     4321 cggtcgacgg cgacgtggtg cagcggcgcg gggcgttcgg ctccgggccc tcgctctaca
     4381 tccaggaccc ggacggctac cggatcgagc tcaagccccg ctgaccgttc cgccgacggc
     4441 gagagtcaag agggtgcgga cgatctgctc ggcggtcacc gcgccgccgt cggtggcggc
     4501 ctggagcagg cagccctcgg ccgcgccgac gatcaccgcg gccaccgcgg ccgcgtccat
     4561 cccggcgcgg aaggctccgg cgcggacgcc ttccaccagg atgtcctcca gggagaggcg
     4621 gatctgctcg cgctgccagg cgagggtcaa ccggaactgc ttgtcgtgcc gcgcccgcat
     4681 ccacccgtgg aggaagagtc ccgagaagtc gggatcgctc tcgccgtagg ccccgaagag
     4741 gatctccatc gcgccttcca gcttcacgcg cggcgcgccc gggccgctcg ccacgcgggc
     4801 cagctccgcc acgatgggca ggccgcgctg ctcgacctga ctggccgcgg ggacggtggc
     4861 cgccggggcc atgggcggcg cggcgggagc cgggacggga gccggaatcg cgcggccccg
     4921 acgggcgcgg gagcggatcc ggcggcgggg acggggacgg gcggggcggc gcggtttccg
     4981 tttcttcaca tccgggcagg tcagttcccg gaggacacga actggaagga ctcgatctcc
     5041 acgcggaagc ggcgctcgga gcccggggac gacatgcgga cctcgaaggg cgaggagcct
     5101 cgcgacggta cgacgccgaa gacataggcg cggccacggc tcaccacgcg cccgctctca
     5161 tcgatcccct cgacgctcac gacgacgcgc tcacaggatg cgttggtgcc gttccgcacc
     5221 tcgccgtaga tcaggacgcg cccgctcccc acccgctcgg tcgtgaagct caccgccagg
     5281 cgcgacgaag attggggcgt gtaggtctgg gcctgggcgg ccggggcgag cgcgaggagg
     5341 atccccgccg ccgcgagcag agcgcggagc atcattcgta ccggagcgcg tcgatcgggt
     5401 tgaggccggc cgccttgcgc gccggataga acccgaagaa gatgccgacc gcggccgaga
     5461 agccgaaggc gagcaggatc gcgtcgaact gcagcagggt gcgccactcc gcgaagtagg
     5521 cgatcgcgta ggagccgagc aggcccgccc cgacgccgat gaccccgccg atgagggaca
     5581 gggtcaccgc ctccaccagg aactgggtga ggatgtcctt gcctcgcgcg cccaccgcca
     5641 tgcggagccc gatctcccgc gtccgttccg tcaccgagac cagcatgatg ttcatgatac
     5701 cgataccgcc caccagtagc gagaccgatg cgatggcggc caggaggacc gtgaggaccc
     5761 gggaggccgc ctcctgcgac tggagcacct cggccaggtt ccggaggttg aagtcgtctt
     5821 cctggtgagg ctggaggcgg tggcgctgtc gcagcagctc ccggatctcg aactccgcct
     5881 ccttcatgag gcccgcgtcc cggaccttga tgctgatggc gcccaccgcg cgtcccttgg
     5941 cctggttgcc gcccacgacc ttggtcttgg cggtggacag cgggatcagg atctgatcgt
     6001 cctggtcctg cccccaggtc gtctgcccct tgcgatccat gaccccgatg atggtgaagg
     6061 gcaccctctt gatccggatg acctgaccga gcgggtcttc ctcgccgaac aggttctgcg
     6121 cgaccgtctg cccgaccagc gcgaccttga acgctccctc cacctcctct cgcgtcagcg
     6181 agcgcccggc gctcagctcc caatcgcggg ccgggaggta ctcgggggtc acccccagga
     6241 tggtggtgga ccagttgagg ttggagtaga cgacctgggc gccgccgcgg acggacgggg
     6301 cgaccacctc gacgagcgcg acatcgcgct cgatggccaa ggcgtcgtcc tcggacagcg
     6361 tggcctgggc gccggcgccg agtcgcaccc cgcccaggtt gacggtgccg ggccagatga
     6421 tgatgagatt cgcgcccagg ctctggatct gctcggccac ccgggcctgc gccccggcgc
     6481 ccaccgccac catggtgatg accgcgccca ctccgatgac gatgcccagc atggtgagcg
     6541 cgctccgcag cttgttgacc cggagggccc gtaccgcgat acgcgcgctg gcgaggacgt
     6601 tcacggggac gcgacctccg cctccacgtc ttcctcctcc tgctcctctc cgcggggcag
     6661 gctggcgagc tgctcgcgcg ccaccagccg gtcgcggacc tcttcgtccc ggatcagccg
     6721 gccgtcacgg aagcccagcg cccggccggc gtatcgcgcg atatcggcct cgtgggtcac
     6781 caccaccacc gtgatgccct cgcgattgag gtcctggaag agcgccatga tctcgacgct
     6841 ggtgtgcgag tcgagcgccc cggtgggctc gtcggccagg atcaggaccg gatcgttgat
     6901 gagggcgcgc gcgatggcca cccgctgctg ctgcccgccc gagagctggg ccgggtggtg
     6961 gtgctcccgc tccgacaggc cgaccgcggc cagcttggcg cgggcccggg cctgtcggtc
     7021 gcgaccctgg atcgcactgt agagcagggg cagctccacg ttctcgagcg cggaggtgcg
     7081 cggcaacagg ttgaaggtct ggaacacgaa gccgatcttc tcgttgcgca cccgcgcgag
     7141 ccggtcgcgg cccatgcggg agacgtcgag gccgtcgagg cggtactgac cggaggtcgg
     7201 ggtgtccagg cagccgagca ggttcatgaa ggtcgacttg ccggagccgg acggtcccat
     7261 caccgccacg aactcgcccg gctggatgga gatcgagacc ttgcggagcg cgtggatccg
     7321 ccgcgtgccg aggtggtagt ccttcacgag atcctcggtc gcgatgaggg gctcgggcat
     7381 ctcagagcct caggcgcgga gcgccttgcg attgctgcgg ccggtcgccc ggggccacga
     7441 cgccgacgat cacctgctgg ccttccttga tctccccctt ggccacctcg gtgtaggtgc
     7501 cgtcgctgat gccgagctgc agcgcgagcg cggccggctt gccgtcggac ccgaccaccc
     7561 acacgcgccc gacgggcgcc gcgccaccgc ggccgccgcc accgcccggc cgggcgggcc
     7621 gctcacgctc gggggcttcc acgccggctg gccggaagcg gagcgccgcg ttcggcacct
     7681 ggagcacgcc ctccttctgg tcgatgatga tccgcacgtt ggcggtcatg ccgggcagga
     7741 gccgctgctc ggggttctta gcggtcacca ccacgtcgta ggtgacgacg ttctggacca
     7801 cgagcggggc cttccggatc tggacgacct caccggtgac ggtccggccc gggaaggagt
     7861 ccaccgtgaa ggtggcggtc tggccgacct tgacccggcc cacgtccgcc tcgtccacgt
     7921 tggtgtccac ctgcatgttg cgcaggtcct gggcgatcgt gaacagcgtc ggggcctgga
     7981 ggctggccgc gacggtctgg cccacgtcca cgctgcgccc caccacggtg ccgtccaccg
     8041 gggcgcggat gtaggtgcgc tcgagatcca cctgcgcctg acgcagggcc gcctggctct
     8101 ggacgacctg ggccgccgcg ttcttcagca tcgcctccga gacgcggagc tgcgcctcgg
     8161 ccgcccgcac cgtcgagacc tgggcgtcct cctgggcctt cgaggcctcc acctgcgcca
     8221 ccgcggagtc gtaggcggcc tgggcggtgt cctcgtcggc ctgcgcgatg aggccgcgct
     8281 tgcgcagatc ctggttgcgg ccgagcgtgc gcttggagtc gacgaccgcg acctgggcct
     8341 tggcggtctg ggcgcgtgcg gccgcgaacg cggcgcgggc gttctcgagg tccgcgcgcg
     8401 tcttctccac caccgcctgc tggttcagca ccgaggcctg ggtcgcgtcc acctgcgcct
     8461 tcgcctggct caccttcgcc tcgtaggtct cgggatcgat gcgggcgatg acctggtcct
     8521 tcttgacctg cgagttgaag tccgcgaaga gctccttgac ctgcccggag atctgggagc
     8581 cgaccagcac cgtgatgacc gcgttgagcg tgccggtggc cgagaccgcg gaggtgagcg
     8641 gaccgcgcga caccgtggcg gtgcggaagg tcgcgcgggc cccgatgccc tgggtataga
     8701 gatagccccc ggagatcact cccgcgccga gcacgatccc gacgatccag agcaggcggc
     8761 gcttcatggc gatcagacgc gtcgggggcg cggggtattc ctagcggcgg ccgagggacc
     8821 gctccaggtt ggcgat
//
LOCUS       DASUFA010000410         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1360429, whole genome shotgun sequence.
ACCESSION   DASUFA010000410 DASUFA010000000
VERSION     DASUFA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360429"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /gene="tilS"
                     /locus_tag="VFX87_14835"
     CDS             <1..240
                     /gene="tilS"
                     /locus_tag="VFX87_14835"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX4996257.1"
                     /translation="AGDRFLPWGGPEERRLKTFLIDAGVPRWERSRIPLLEADGEIIW
                     VAGLRRGRAAPVTGDTRRILEVTLASPLAVPTARG"
     gene            264..1751
                     /locus_tag="VFX87_14840"
     CDS             264..1751
                     /locus_tag="VFX87_14840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX4996258.1"
                     /translation="MVIVRGIFTSGALGTLALTALLLLLTPAPSPAQRSAPAPPEPAR
                     ILEALETAFVAVADRVMPAVVNVNVKSKRSPAVSEAPERPEEMDERFKEFFGQEFFDR
                     FFRRRSPRDEGRASGSGVIVDPRGYILTNSHVVETATDIEVGLSDDRKFKATLVGRDA
                     KTDVAVLKIEAPLGSLPVAELGDSDRLKVGQWAVAIGNPFGLDRTVTVGIISATGRTR
                     VGVATYEAFIQTDASINPGNSGGPLLNLQGRVVGINTAIVATGQGIGFSIPINMAREV
                     MAQLIARGRVVRGWLGIVIQDLTPDLAAGFDLPNAGGVLVSDVMKDGPAESAGMRAGD
                     VIVEFAGTPIKEVTDLQKRVAAVEPGKPAALTVIRDKRPTRLSVRIGEQPLEEVSVAA
                     PPREERLGLTVEPLTVDTAERFGLSARAGVVVTDVKPGSAGAEAGIRPGDAILEVNRR
                     PITGMESFRESMAAVAPGDVVPVYIQRGGGGNEYVVLKSADPTKP"
     gene            1757..>2276
                     /locus_tag="VFX87_14845"
     CDS             1757..>2276
                     /locus_tag="VFX87_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4996259.1"
                     /translation="MSAEPDPTHVYRPHPVVLVIDDEPGVRESLREILKHDFEVLEAE
                     DGAAGLPLVRSRRVDVVMLDVRLPGERGPLVLPKILALDDSIAVILMTAVPDVRTAVD
                     AIKAGAYDYIVKPFDVDEIHLLVRQAAQQRVLEREVCYLRAELDRAHGFDSLVGRHAA
                     MVRLYEQITQVAQ"
BASE COUNT          320 a          798 c          817 g          341 t
ORIGIN      
        1 gccggcgacc ggttcctgcc gtgggggggg ccggaagagc ggcggctcaa gaccttcctc
       61 atcgatgcgg gcgtgccgcg ctgggagcgt tcgcgcatcc cgctgctcga ggccgatggc
      121 gagatcatct gggtggcggg gcttcgccgg ggtcgcgcgg cgccggtcac cggggacacg
      181 cgccgtatcc tcgaggtgac actcgcttct ccgctggcgg tgcccacggc gcgagggtaa
      241 gatggccgcc ggaggctagt cccatggtca tcgtccgcgg gatcttcacg tccggcgcgc
      301 tcggcacgct cgcgctcacc gcgctgctgc tgctgctgac gccggcacca tctcccgccc
      361 agcgatcggc gcccgcgccg ccggagccgg cgcgtatcct cgaggcgctg gagaccgcct
      421 tcgtggccgt ggccgatcgt gtgatgcccg cggtcgtcaa cgtgaacgtc aagtcgaagc
      481 gcagtcccgc cgtctcggag gcgcccgagc gcccggagga gatggatgag cgcttcaagg
      541 agttcttcgg ccaggagttc ttcgaccgct tcttccggcg ccgctcgccg cgcgacgagg
      601 gccgtgccag cggatcgggc gtcatcgtcg atccccgcgg ctacatcctc accaatagcc
      661 acgtggtgga gaccgcgacg gatatcgagg tggggctgtc ggacgaccgc aagttcaagg
      721 cgacgctggt gggccgggac gccaagaccg acgtggcggt gctcaagatc gaagcgcccc
      781 tgggctcgct cccggtggcg gagctgggcg actccgaccg gctcaaggtg gggcagtggg
      841 cggtggccat cggcaatccc ttcgggctcg accgcacggt gacggtgggc atcatctccg
      901 ccaccggccg cacccgcgtg ggagtggcca cctatgaggc gttcatccag accgacgcct
      961 ccatcaatcc gggcaactcg ggggggccgc tgctcaacct gcagggccgt gtcgtcggca
     1021 tcaataccgc catcgtcgcc accggacagg gcatcggctt ttccattccc atcaacatgg
     1081 cgcgcgaggt catggcccag ctcatcgccc gcggccgcgt ggtgcgcggc tggctaggca
     1141 tcgtgatcca ggatctcacc ccggatctgg ccgccggctt cgacctgccg aacgccggcg
     1201 gcgtgctcgt ctccgatgtg atgaaggacg ggcccgccga gagcgcggga atgcgcgccg
     1261 gcgacgtgat cgtggagttc gcggggacac ccatcaagga ggtcaccgac ctccagaagc
     1321 gcgtggccgc cgtcgagccg ggcaagcccg ccgccctcac ggtgatccgc gacaagcggc
     1381 ccacccggct ctcggtccgc atcggcgagc agcccttgga agaggtgtcg gtggcggccc
     1441 cgccgcgcga ggagcgcctc ggcctcaccg tggagccgct caccgtggac accgccgagc
     1501 gcttcgggct gtccgctcgc gccggcgtgg tggtcacgga cgtcaagccc ggcagcgcgg
     1561 gcgcggaggc cggcatccgg cccggcgacg ccatcctcga ggtgaaccgc cgtcccatca
     1621 ccgggatgga gagcttccgg gagtcgatgg cggcggtggc gccgggcgac gtggtgcccg
     1681 tctacatcca gcggggcggc ggcggcaacg agtacgtcgt gctcaagtcg gccgatccca
     1741 ccaagccctg agccccgtgt ccgccgagcc cgatcccacc cacgtctacc ggccccaccc
     1801 cgtcgtgctc gtgatcgacg acgagcccgg ggtccgcgag tcgctgcggg agatcctcaa
     1861 gcacgacttc gaagtgctcg aagcggaaga tggcgccgct ggcctcccgc tggtgcgctc
     1921 gcgtcgcgtg gatgtggtca tgctcgacgt gcggctgccc ggcgagcgcg gccccctggt
     1981 gctgcccaag atcctggcgc tggacgattc catcgcggtg atcctgatga cggcggtgcc
     2041 cgacgtgcgc accgcggtgg acgccatcaa ggcgggcgcg tacgactata tcgtcaagcc
     2101 cttcgacgtg gacgagatcc acctcctggt gcggcaggcg gcgcagcagc gggtgctcga
     2161 gcgcgaggtg tgctatctgc gggccgagct ggatcgcgcg cacggcttcg actcgctggt
     2221 gggccgtcac gccgccatgg tccgcctgta cgagcagatc acccaggtgg cgcaga
//
LOCUS       DASUFA010000411         7650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_790242, whole genome shotgun sequence.
ACCESSION   DASUFA010000411 DASUFA010000000
VERSION     DASUFA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790242"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VFX87_14850"
     CDS             complement(<1..1270)
                     /locus_tag="VFX87_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4996260.1"
                     /translation="MPASGPVLLCINHPNNLIDSLLVGGVLPRKVHYLATAALFRNPL
                     MGRFLTACGAIPVYRKQDDPDKMDRNADAFAACFGALERGQLVGIYPEGTTHSESRVQ
                     RIKTGAARIALGYQASERGAPLTVIPVGITFEHRKAFRGHVRISFGEPIPLAPYARMY
                     RDEAAGAVEALTTTIQWGMEAETLHVGRPERQDLIRAVDEIYRSDLIRELQEERGLGT
                     RQIDPLRLSRAIADAAAHFEERDPERVARLWAAIQKYRAMLAAYQVRDQTVRSRAHRP
                     AARARLRHSWEASIGLPFFAYGLVVNGLPYLLPRWLARRTARKETDYATTRLLASIVA
                     FPLFWAVEIWLVWRLLGSAWALGFALSLPVSGLLAYRYLGGMGRLRNQLRFGVLSFTH
                     RQSATRLLAERQALVAELERAKDDYLAATRG"
     gene            complement(1363..1827)
                     /locus_tag="VFX87_14855"
     CDS             complement(1363..1827)
                     /locus_tag="VFX87_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996261.1"
                     /translation="MTGRRAAFPALALAGALAAAPLSASIVPVPSPLVGEVRAEHQVS
                     YRYVVLGYVTDAGNRGRPGVRVELRREKTGFSYLGETDADGFYLIVARLGDESAGETL
                     HLRADSQAMTLTARFDPADHATERGTRVDFTARRPVESPTAFAGTLKRYLAQ"
     gene            complement(1832..2569)
                     /gene="pncA"
                     /locus_tag="VFX87_14860"
     CDS             complement(1832..2569)
                     /gene="pncA"
                     /locus_tag="VFX87_14860"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEX4996262.1"
                     /translation="MTTNRRQFLSGAVGGLVAAGAAGGTRQAWAQGTAARIKPGGRDV
                     LLVVDVQNDFIPGGALPVKEGDLIVPLVNQIAAGFEHVILTQDWHTPGHISFASSHPG
                     KKPFETTKLGYGTQVLWPDHCVQGTPGADFHKDLKIPHAELVIRKGYRKQMDSYSAFF
                     EADGKATTGLAGYVRDRGFRQVFLVGLATDFCVCWSALDARKVGLGALVVEDACRGID
                     TNGSLAKAWEQMTKAGVKRIRSADLSA"
     gene            2684..2821
                     /locus_tag="VFX87_14865"
     CDS             2684..2821
                     /locus_tag="VFX87_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996263.1"
                     /translation="MEAEGMMRRYAAITGTTLRATVEAVSGTPLTLIPGGLFVVTWWT
                     F"
     gene            complement(2855..3151)
                     /locus_tag="VFX87_14870"
     CDS             complement(2855..3151)
                     /locus_tag="VFX87_14870"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4996264.1"
                     /translation="MATLQSRNRRQHPRTRVAWPVVIETRTQRYPCQVMDISSHGAKV
                     MTNARLRTGSVVRLQIIPPDGTPLRVGALVWRLDGDGVAFFFAHGIHHPFIRAA"
     gene            complement(3303..3644)
                     /locus_tag="VFX87_14875"
     CDS             complement(3303..3644)
                     /locus_tag="VFX87_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEX4996265.1"
                     /translation="MPVHPQWVTTAFEFDGHRIVGYRGVVRGITVRSRNLIVNLGATI
                     QTLFGGNITLYTELCERARQEAFDLMLEHAAQMGANAIVAMRYDANDVAAGVTEVLCY
                     GTAVVIEPVAR"
     gene            complement(3696..4103)
                     /locus_tag="VFX87_14880"
     CDS             complement(3696..4103)
                     /locus_tag="VFX87_14880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4996266.1"
                     /translation="MPKLMLPVNPPSLPKPAGFAHGWEVQGGKTVYLAGQVSLDKEGQ
                     VVGKGDLVGQFRQVCENIKAIVTARGGHMDDIVKLTIYVLSKTEYKARGREIGVVYRE
                     YFGRHYPAMTLVEVRGLYDDDCLIEIEGVAVVD"
     gene            4154..5425
                     /locus_tag="VFX87_14885"
     CDS             4154..5425
                     /locus_tag="VFX87_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4996267.1"
                     /translation="MARPFAQVTLADAVGRLRPGMRVLLAPGCGDPSALVAELLRQAD
                     RLAPLTLMGGLRLDDHPFGAPAYAGKIRFATWHMSPRLAAAAARGDVDFVPARYFDTV
                     TLFAAGGPWAPDAVLVHAAPPDRGGHLSLGVSVSYLLPAARRAPLVIAQVNPRMPRTL
                     GNAFLHRSQVDAWTPVDHALLEYPPTPVGEVERRIAGYVADLVPDGATLQVGVGSIPQ
                     AVMEALAGKKDLGVHSLLVEHMLPLVRSGVITNARKRLHPGRMDVGEIMGTAALFEWS
                     HENRLVNMEPSDVVHDPEVVGALGDFVSVNSALEVDLLGQVNAESVDGRQVTGIGGQF
                     DFVLGAARAEGGRSIIALPATASRGTLSRIVARLAAGARVTTPRFLADYVVTEHGVAA
                     LRGKSDAGRARELLHIADPAFRDRIEREIRP"
     gene            complement(5415..5597)
                     /locus_tag="VFX87_14890"
                     /pseudo
     CDS             complement(5415..5597)
                     /locus_tag="VFX87_14890"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            5714..6694
                     /locus_tag="VFX87_14895"
     CDS             5714..6694
                     /locus_tag="VFX87_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX4996268.1"
                     /translation="MTQHLLIDTDPGIDDALAILLALRSPDARVEAVTTVAGNVAVDR
                     ATANARRILAVAAPDPRPLLAGGAPAPLKRALVTAHQVHGQDGLGNLERFVEPTGQPR
                     YPEPAYEIEMRSGPEVILEAADRWGAELTIVALGPLTNLALALQQDPRRLGRSGRIVV
                     MGGAVAVPGNITPAAEFNFYVDPEAAAVVLDAGLSVELVPLDVTRRVVLAQAALSERL
                     LRCSDRIARFILDFTLHGFAFGAERERGGIVLHDPLAMAVALDSSLVTLEPLSVEVEC
                     EGRLTRGLSLADRREIPSHRKRAPTCRVAVDVDAERVLRMVLERLCPVSA"
     gene            6676..7602
                     /gene="rbsK"
                     /locus_tag="VFX87_14900"
     CDS             6676..7602
                     /gene="rbsK"
                     /locus_tag="VFX87_14900"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX4996269.1"
                     /translation="MPRICVIGSANLDFTVALPRLPHVGETVSAGTLLVNRGGKGANQ
                     AVAARRLGAEVRMIGCVGDDDSGGDIRRGLLEEGIGVEGLVTTPDAATGTALIFVDRE
                     GRNQIGVAPGANHRLTVEMARRGEDAILWAEVVLCQLEIPVPVVRWALETARRHGITT
                     VLNPAPVQELSDELLALVDYLTPNAGEVMALTGIEVQDLDTGRQAAGRLVERGVGTVI
                     ITLGEEGALVCDGGSVVHFEAFPVEVIDTTGAGDAFNGALAVGLAAGGSLEQIIPLAS
                     AAAALTCTRRGAQDALPTRADVERFLQSLRNR"
BASE COUNT         1096 a         2673 c         2709 g         1172 t
ORIGIN      
        1 tcccccgcgt ggccgccagg tagtcgtcct tggcgcgctc cagctcggcc accagggcct
       61 gccgctccgc caggaggcgg gtcgcggact ggcgatgggt gaaggagagc acgccgaagc
      121 ggagctgatt gcgcagccgg cccatccccc cgaggtaccg gtaggcgagc aggccgctca
      181 ccggcaggga gagcgcgaag ccgagcgccc acgccgagcc caggagccgc cacaccagcc
      241 agatctcgac cgcccagaag agcgggaacg ccacgatgct cgccagcagt ctggtggtgg
      301 catagtccgt ctccttgcgc gcggtgcggc gggccagcca ccgcggcagg agatagggca
      361 agccgttgac gaccaggccg taggcgaaga agggcaggcc gatcgaggcc tcccacgagt
      421 gccgcagccg ggcgcgcgcg gccggacgat gggcgcgact gcgcaccgtc tggtcccgca
      481 cctggtaggc ggccagcatg gcgcgatact tctggatcgc cgcccacagc cgcgccaccc
      541 gctcgggatc gcgctcctcg aagtgggccg cggcgtccgc gatcgcgcgc gagagtcgca
      601 gagggtcgat ctgtcgggtg ccgagcccgc gctcctcctg cagctcccgg atcaggtcgc
      661 tgcggtagat ctcgtccacg gcgcggatca gatcctgccg ctccggccgg cccacgtgga
      721 gggtctcggc ctccatgccc cactggatcg tcgtggtcag ggcctcgacc gccccggcgg
      781 cctcatcccg gtacatgcgc gcatagggcg ccagcgggat gggctccccg aacgagatcc
      841 gcacatggcc gcggaaggcc ttgcggtgct cgaaggtgat gcccacgggg atcacggtca
      901 gcggcgcccc gcgctcggag gcctggtagc ccagcgcgat gcgcgcggcg ccggtcttga
      961 tgcgctggac gcggctctcc gaatgggtcg tgccctccgg gtagatgccg acgagctgcc
     1021 cacgctcgag ggcgccgaag caggcggcga aggcatcggc gttgcggtcc atcttgtcgg
     1081 gatcgtcctg cttgcggtag acggggatgg ccccgcaggc cgtgaggaac cggcccatca
     1141 ggggattgcg gaagagggcg gcggtggcca ggtagtgcac cttccggggc aggacccccc
     1201 cgaccagcag ggagtcgatc aggttgttgg ggtggttgat gcagaggagc acgggccccg
     1261 acgccggcac cctgtccgca cggaccgcgt cgagcttctt gaagaaaaac cacagccaga
     1321 acctgccaac ggaccggact gtggagtaga accgatcgtc actcactggg cgaggtaccg
     1381 cttgagcgtg cccgcgaagg cggtcgggct ctcgaccggc ctgcgggcgg tgaagtccac
     1441 acgcgtgccg cgctcggtgg cgtggtcggc ggggtcgaag cgcgcggtca gggtcatggc
     1501 ctggctgtcc gcgcggaggt gcagggtctc gccggcgctc tcgtcgccga gtcgggcgac
     1561 gatcagatag aaaccgtcgg cgtccgtctc gcccaggtag gagaacccgg tcttctcgcg
     1621 ccgcagctcg acccgcacgc cgggccgccc ccggttgccc gcgtcggtca cgtaaccgag
     1681 cacgacgtac cggtaggaga cctggtgctc ggcccggact tccccgacga gcgggctcgg
     1741 gacggggacg atggacgcgg aaagaggggc cgcggccagc gcaccggcca gcgcgagcgc
     1801 cgggaaggcc gcgcggcggc ccgtcacgcc cctaggccga caggtcggcg gagcggatgc
     1861 gcttcacccc ggccttcgtc atctgctccc acgccttggc cagcgagcca ttggtgtcga
     1921 tgccgcggca ggcgtcctcg accaccagcg cccccaggcc gaccttgcgc gcgtccagcg
     1981 ccgaccagca gacgcagaag tcggtcgcga ggccgaccag gaacacctgg cggaacccgc
     2041 ggtcgcgcac gtagccggcg agcccggtcg tggccttgcc gtcggcctcg aagaaggccg
     2101 agtaggagtc catctgcttg cgatagccct tgcggatgac cagctccgcg tgcgggatct
     2161 tgagatcctt gtggaagtcg gcgccgggcg tgccctgcac gcagtgatcc ggccagagca
     2221 cctgggtgcc gtagccgagc ttggtggtct cgaacggctt cttcccgggg tgcgaggacg
     2281 cgaacgagat gtgacccggc gtgtgccagt cctgggtcag gatcacgtgc tcgaagcccg
     2341 cggcgatctg gttcacgaga ggcacgatca gatcgccctc cttcaccggc agcgccccgc
     2401 ccgggatgaa gtcgttctgg acgtccacca cgaggagcac gtcacggccg cccggcttga
     2461 tcctggcggc ggtaccctgg gcccaggcct gccgggtccc gccggccgcg cccgccgcca
     2521 ccagacctcc caccgcgccg ctcagaaact gtcgccgatt cgtggtcatg atgtcttctc
     2581 cccccgggcc gctgtcggcc cgcaccccgc ccgagagtat agtcccgcct ccgtccgggc
     2641 aaggctgaag aatctcggcg ccgactatga tgtagctcgg cgcgtggaag ccgaggggat
     2701 gatgcggcgc tacgcggcta ttactgggac aacgctgcgc gcgaccgtgg aagcggtgag
     2761 tggaacaccg ctgacgctga tacccggcgg attgtttgtt gtgacgtggt ggacgttctg
     2821 aggtgttcgg gcttcaggct ttgggcgtac tccactacgc ggcgcgaatg aacgggtgat
     2881 ggatgccatg agcgaagaag aaggccacac catccccatc caggcgccac accaatgccc
     2941 cgacacgcag cggtgttccg tccggcggga tgatctgcaa gcgcacgacg ctcccggtcc
     3001 ggagccgcgc gttggtcatg accttcgcgc catggctgct gatgtccatg acctggcaag
     3061 gatagcgctg agtcctcgtt tcgatcacca cgggccacgc gacccgggtc cgtgggtgct
     3121 ggcgtcgatt ccgagactgg agcgtggcca tgggctgacg tttcgcaact tgcgtgccca
     3181 gaccacaaca ccatgggcaa gcatagttag gggcacgacc ctccctaagt gtcagattcc
     3241 cgtagctgtc gtgctcccgt gacgtgacag atgtcacgtg ggaaacaccc tggtgggcgg
     3301 tctcagcgcg ccaccggctc gatcacgacc gcggtgccgt agcagagcac ctccgtcacc
     3361 cccgcggcca cgtcgttcgc gtcgtagcgc atggccacga tcgcgttcgc gcccatctgg
     3421 gccgcgtgct ccagcatgag gtcgaaggcc tcctggcgcg cccgctcgca cagctcggtg
     3481 tagagcgtga tgttgccgcc aaagagcgtc tggatggtgg cgcccaggtt gacgatgaga
     3541 ttgcgcgagc gcaccgtgat gccgcggacc acgccccgat agcccacgat gcgatggccg
     3601 tcgaactcga acgcggtcgt cacccactgc ggatggacgg gcatggcttc cccctcaccc
     3661 taccttctcc ctcgagggga gagggtggtt ggaactcagt ccacgaccgc gacgccttcg
     3721 atctcgatca ggcagtcgtc gtcgtagagg cctcggacct ccactagggt catggccggg
     3781 tagtgccggc cgaagtactc gcggtagacc acgccgatct cgcgcccccg cgccttgtac
     3841 tcggtcttgg agagcacgta gatggtgagc ttgacgatgt cgtccatgtg gccgccgcgc
     3901 gcggtcacga tcgccttgat gttctcgcag acctggcgga actggccgac cagatcgccc
     3961 ttgccgacga cctgcccctc cttgtccagg ctgacctggc ccgcgaggta gacggtcttg
     4021 ccgccctgga cttcccagcc gtgggcgaaa ccggccggct ttgggagact cggtggattg
     4081 acgggcagca tgagcttcgg catggattcc ccctctcccg tgagtggctg cgcgattata
     4141 ctacccgggc tccatggccc gccccttcgc gcaggtcacg ctcgcggacg ccgtcggccg
     4201 tctccgcccc ggcatgaggg tgcttcttgc gccgggctgc ggcgacccct ccgcgctcgt
     4261 cgccgagctc ctgcgacagg ccgaccggct ggctccgctg accctgatgg ggggcctgcg
     4321 cctcgacgac catcccttcg gcgcacccgc ttacgcgggc aagatccgct tcgcaacctg
     4381 gcacatgtcg ccccgcctcg cggcggcggc cgcgcgcggc gacgtggact tcgtccccgc
     4441 gcgctacttc gacacggtga ccctgttcgc ggcgggtggg ccctgggcgc ccgacgcagt
     4501 cctcgtgcac gcggcgccgc ccgaccgcgg cggccatctc tccctcggcg tctcggtgag
     4561 ctacctgctg cccgcggcgc gccgggcccc cctcgtcatc gcccaggtca atccgcgcat
     4621 gccgcgcacc ctgggcaacg cgttcctcca tcgctcgcag gtggacgcgt ggaccccggt
     4681 ggaccacgcg ctgctcgagt acccgccgac cccggtcggc gaggtcgagc gccggatcgc
     4741 cgggtacgtg gccgacctgg tcccggacgg ggcgacgctt caggtgggcg tgggctcgat
     4801 cccgcaggcg gtgatggagg cgctcgcggg caagaaggac ctcggcgtgc attccctcct
     4861 ggtcgagcac atgctgcccc tggtgcggag cggcgtgatc accaacgcgc ggaagcgcct
     4921 gcatcccggc cgcatggacg tgggcgagat catgggcacc gccgcgctgt tcgagtggag
     4981 ccacgagaac cggctggtga acatggagcc ctccgacgtc gtgcacgacc ccgaggtggt
     5041 gggggcgctc ggcgacttcg tctcggtgaa ctcggctctc gaggtggacc tgctcggcca
     5101 ggtgaacgcc gagagcgtgg acgggcggca ggtgacgggc atcggcggcc agttcgactt
     5161 cgtgctgggg gcggcgcgcg cggaaggggg ccggtccatc atcgcgctgc ccgccaccgc
     5221 gagccgcggc acgctctccc gtatcgtggc gcgcctggcc gcaggagcgc gggtgaccac
     5281 cccgcgcttc ctggcggact acgtggtcac cgagcacggg gtggcggcgc tgcggggaaa
     5341 aagcgacgcc gggcgggcgc gggagctgct ccacatcgcc gacccggcgt tccgggaccg
     5401 tatcgagcgc gagatcaggc cttgagcttg tcgatctcgg tgaagatctc ctggtcctcg
     5461 ggaaagcgga aggtgatctg gttgtcgtag atcatcttct cgtcggcctc gacggtgaag
     5521 atgcccccga cgccttcccg cagctcggag tcaatgccgt acttactctt gatcgcggcc
     5581 tgcaaactgg aggcctgggg gagatagttt cactcgccgc agtacgtgat ccggatatgc
     5641 atccgagtcc tccttggcgc caagtttgcg ccggactgtc gcgtatgata cgaacgcccg
     5701 cgggggctgt caaatgaccc agcacctcct gatcgatacc gaccccggca tcgacgacgc
     5761 gttggcgatc ctgctcgctc tgcgctcgcc cgacgcgcgc gtggaggccg tgaccaccgt
     5821 cgccggcaac gtcgcggtgg atcgcgcgac cgcgaatgcc cgccgcatcc tcgcggtcgc
     5881 cgcgcccgat ccgcgtccgc tgctggccgg cggcgccccg gccccgctca agcgggcgtt
     5941 ggtcaccgcg catcaagtgc acggccagga cgggctcggg aacctcgaac ggttcgtcga
     6001 gcccaccggg cagccccgct acccggagcc tgcctacgag attgagatgc gatcgggccc
     6061 ggaagtgatt ctggaggcgg cagatcgctg gggcgccgag ctgaccatcg tcgccctggg
     6121 gccgctgacg aacctggcgc tggccctcca gcaggatccg aggcggctcg gccggtccgg
     6181 ccggatcgtg gtcatgggcg gcgcggtcgc ggtgccgggc aacatcaccc ccgccgccga
     6241 gttcaacttc tacgtggatc cggaggcggc cgcggtggtg ctggacgccg gcctttcggt
     6301 ggagctcgtc ccgctcgatg tgacccgccg ggtggtgctc gcgcaggccg cgctctccga
     6361 gcggctgctg cgctgctccg accggatcgc ccgcttcatc ctggacttca ccctgcacgg
     6421 cttcgccttc ggagcggagc gggagcgcgg cggcatcgtg ctgcacgacc cgctggccat
     6481 ggcggtggcg ctcgattcct cgctggtcac cctcgagccc ctctccgtcg aggtcgagtg
     6541 cgagggcagg ctcacgcgcg gcctctcgct cgccgatcgg cgcgagattc cctcccaccg
     6601 caagcgcgcg cccacctgtc gcgtggcggt ggacgtggac gccgagcgcg tgctgcgtat
     6661 ggtcctggag cgcctgtgcc ccgtatctgc gtgatcggct ccgcgaacct ggacttcacg
     6721 gtggccctgc cgcgtctacc ccacgtgggc gagacggtct ccgcgggcac gctcctggtg
     6781 aaccggggcg gcaagggcgc caaccaggcg gtggcggcgc ggcggctggg cgccgaggtg
     6841 cggatgatcg gctgcgtggg cgacgacgat tcgggtggcg acatccgccg ggggctgctg
     6901 gaggagggga tcggcgtgga ggggctcgtg accacgccgg acgcggcgac cggcaccgcc
     6961 ctgatcttcg tggaccgcga ggggcgcaac cagatcggcg tcgccccggg ggccaatcac
     7021 aggttgaccg tcgagatggc gcggcggggg gaggacgcga tcctgtgggc ggaggtcgtg
     7081 ctctgccagc tggagatccc ggtgccggtc gtgcgctggg cgctcgagac ggcgcggcgg
     7141 cacgggatca ccaccgtgct caacccggcg ccggtgcagg agctatccga cgagctgctg
     7201 gccctggtgg actacctcac gccgaacgcg ggcgaggtga tggcgctcac cggcatcgag
     7261 gtgcaggatc tcgacacggg ccggcaggcg gcgggccggc tcgtcgagcg cggcgtgggc
     7321 accgtgatca tcacgctcgg cgaggagggc gcgctcgtgt gcgacggcgg ttcagtcgtg
     7381 cacttcgagg ccttcccggt ggaggtgatc gacaccaccg gcgccggcga cgccttcaac
     7441 ggcgccctcg cggtcggcct cgccgccggc ggcagcctcg agcagatcat ccccctggcg
     7501 agcgccgccg ccgccctgac ctgcacgcgc cgcggcgccc aggacgccct ccccacgcgc
     7561 gccgacgtcg agcgcttcct ccaatccctc cgcaaccgct agcccggggt caggtcttac
     7621 attccgacat ttccccggca gcgacgttcg
//
LOCUS       DASUFA010000412         6658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1018689, whole genome shotgun sequence.
ACCESSION   DASUFA010000412 DASUFA010000000
VERSION     DASUFA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018689"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..703)
                     /locus_tag="VFX87_14905"
     CDS             complement(68..703)
                     /locus_tag="VFX87_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX4996270.1"
                     /translation="MDPQLWAFLLVATLLAVTPGADTLLVARNVLGRGRTAGLTTIAG
                     IAAGCFVHAALSAVGVSLILVRSAEAFHALKWGGAAYLIILGIQSIRGWWRADEVAPA
                     AAADRERAGSRRLRSFLEGLLTNVLNPKVGLFYLAFLPQFIRPGDPVLARSLLLGALH
                     VGIGVLWLSLLSLSLVRIRPLVESRLWRARLEGASGAVLIALGVRLAAERR"
     gene            831..1853
                     /locus_tag="VFX87_14910"
     CDS             831..1853
                     /locus_tag="VFX87_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4996271.1"
                     /translation="MTARQARRGGARILAMALGLAAGGVAAPGESAEVMVYSARAHYG
                     QEPAMEAFTKKTGIVVKSFGGESGPLFERLKAEGDRTPADVLISVDAGNLWNAARAGL
                     LGKVDSPEIQANIPAHLRDPESRWVGLTVRARTIMYNTKKVKPEELSTYEALGDPRWK
                     GRLCLRSSTHIYNQSLLATMIRRYGEAKAESIVRGWVANNPTLIQGDTKILEAVAAGQ
                     CDVALTNTYYLGRILAKDATFPVAAFWANQQTTGTHVNVSGAGVTTHAKNRAEAIRLI
                     EFLTSPEAQQMFADSNFEYPANPQAGVNPLIAGWGKFKQDDTNIAAAGEFQAAATKLA
                     DRAGYK"
     gene            1903..3471
                     /locus_tag="VFX87_14915"
     CDS             1903..3471
                     /locus_tag="VFX87_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4996272.1"
                     /translation="MLAVPIAAVVFSLAIPAGDVWLHLWRTQLLELVRQTGLLLAGVG
                     IGTLMVGGGLAWLVVHHRFPGRGVLEWALILPLAVPAYVIGFAFIGLFEYAGPLQSEL
                     RRWLGPGARLPEIRSYAGVVVMMTLVFYPYVYLLARVAFREQGAASVETARMLGRSRA
                     GAFLHITVPMARPALVAGVALAMMEALADFGTVTSFGYRTLTEAIYRVWYGMFDRIAA
                     TQLASVLLLFAAGLLLLERHSRGRARFAQSGRRGPAVEPMRLAGWRAAAATGACLGVL
                     GLAFLLPVGQLAWWAVVAIRAGQVAPDFATLLWHTCALAGATAAVASLLAVLLAYAGR
                     LHGTATVRVAAQLASMGYALPGAVIAVGVLLPLAWADHALVPSLERALGRPLGLLLTG
                     SAAGLILAYVVRFLAVGLQTVDASLGKVPPSLDDAARVLGVRAGAALRRVHLPLIRGG
                     LLTAVVLVFVETMKEMPATLLLRPIGLNTLAVEIWERTAESMWPEAAVPALTLVAVGL
                     LPVVLLIRLTSSRP"
     gene            complement(3554..4588)
                     /locus_tag="VFX87_14920"
     CDS             complement(3554..4588)
                     /locus_tag="VFX87_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996273.1"
                     /translation="MDLLLLDRVSKRFGSADPPAVDGLSLRVAEGEILALLGPSGCGK
                     TTTLRLIAGLESPDAGTITLRGQVVAGPGRAVPPEERGIGIVFQDYALFPHLTVADNV
                     AFGLPRVARRSRVEPVLDLVGLGGFDARYPHELSGGQQQRVALARALAPAPALMLLDE
                     PFSNLDADLRAQMRDEVERILRTSGTTAVFVTHDQEEAFTLADRVGVLHAGRIEQIAP
                     PQELYHRPATRFVAAFVGAADFLPGMVTAQGIVTEVGTFANVDQLPAGGRVDVMIRPD
                     DIHFVPQADGTGTITRRYFRGSETVYCIRLLSGDRVHSSQPSASVLPTGMRVRAEARV
                     LHVVAFPLQG"
     gene            complement(4591..5586)
                     /locus_tag="VFX87_14925"
     CDS             complement(4591..5586)
                     /locus_tag="VFX87_14925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX4996274.1"
                     /translation="MATGTGRPAVLISRQLPEPAVQLARSRAEVDAYAKDAPMPRAEL
                     LERLTGKQGLICVISEVVDEALLAARPGLRVVSNVAVGYNNVDVAACTRRGVVVTNTP
                     DVLTDTTADFAWTLLMATARRLVEADRYTREGRFRQWEYMLLQGGDVHGKTLGVVGFG
                     RIGRAMARRALGFGMRVLYQDAVAADPATERELNATRVDLATLLRESDFVTLHTPLIP
                     ETRHLINADSLRTMKKTAYLINAARGPVVDEAALVQALTEGRIAGAGLDVFEEEPAVH
                     PGLIALPNVVLAPHIASASHETRLQMATLAVENCLAVLEGKTPPTPVNPEVLKRA"
     gene            5746..6033
                     /gene="groES"
                     /locus_tag="VFX87_14930"
     CDS             5746..6033
                     /gene="groES"
                     /locus_tag="VFX87_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX4996275.1"
                     /translation="MKIRPLHDRILVKREEEKEVKKGGIIIPDTAKEKPQEGKVVAVG
                     GGKVNEDGKKIPLDVKAGDKILFGKYSGSEVKLDDEEYLILREEDVLAIIE"
     gene            6126..>6658
                     /locus_tag="VFX87_14935"
     CDS             6126..>6658
                     /locus_tag="VFX87_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX4996276.1"
                     /translation="MPKQVMFSDEGRAALLRGVNIMAAAVKATMGPKGRNVVIDKKFG
                     SPTITKDGVTVAKEIELKDNYEDMGAQMVKEVASKTSDIAGDGTTTATVLAHAIIREG
                     LKNVTAGANPMGLKRGIDKAVDAVVDDLKRMSKSTKDKKEIAQVATIASNNDKTIGSL
                     IAEAMEKVGKDGVITVE"
BASE COUNT         1030 a         2267 c         2431 g          930 t
ORIGIN      
        1 cgcgtgcagg cgtccttcga gcaccgcctg gagcggctgg gccggcggct cgagcggctc
       61 gcgggcctca gcggcgctcg gcagccagcc tgaccccgag cgcgatcagc accgccccgc
      121 tcgcgccttc gagccgggcg cgccagagcc ggctctccac cagcggacgg atgcgcacga
      181 gcgagagcga cagcagcgag agccagagga cgccgatgcc gacgtgcagc gcgcccagga
      241 ggagcgaccg cgcgagcacc ggatcgcccg gccggatgaa ctgaggcagg aaggccagat
      301 agaagagccc caccttcggg ttcagcacgt tggtgagcag tccctccagg aaggaccgga
      361 gccgccgaga tcccgcccgc tcccggtccg ccgcggcggc cggcgccacc tcgtcggccc
      421 gccaccagcc ccggatggac tggatgccca ggatgatgag gtaggccgcg ccgccccact
      481 tgagcgcgtg gaaggcctcc gccgagcgca ccaggatgag cgagacgccg accgcggaga
      541 gggcggcgtg gacgaagcag ccggcggcga tgccggcgat ggtcgtgagc ccggccgtcc
      601 ggccgcgacc gaggacgttg cgtgccacga gcagggtatc cgcgcccggc gtcaccgcga
      661 gcagggtcgc caccagcagg aaggcccaga gctgtgggtc catcgggccc attctagccg
      721 cacctggccg cccggaccgg tcctgcggtt ttaggtttga cacacttcga tgattaggct
      781 agccttactc cccgctccac ccaccacggc acggcgaggg agagacgacc atgacggcac
      841 ggcaggcgag gcgaggcggg gcccggatcc tggcgatggc gctcggcctg gccgccggcg
      901 gcgtggccgc cccgggcgag agcgccgagg tgatggtgta ctcggcgcgc gcgcactacg
      961 ggcaggagcc ggcgatggag gccttcacca agaagaccgg catcgtcgtc aagagcttcg
     1021 gcggtgagtc gggcccgctc ttcgagcgcc tgaaggcgga gggcgacagg acgccggccg
     1081 acgtgctgat cagcgtggac gcgggcaacc tctggaacgc ggcgcgcgcg gggctgctcg
     1141 gcaaggtgga ctcgcccgag atccaggcca atatccccgc ccacctgcgc gatccggaga
     1201 gccggtgggt cggactcacg gtgcgcgcgc ggaccatcat gtacaacacc aagaaggtga
     1261 agccggagga gctatccacc tacgaggcgc tcggcgatcc caggtggaag ggccggctct
     1321 gcctgcggag ctcgactcac atctacaacc agtcgctgct ggccaccatg attaggcggt
     1381 acggcgaggc caaggcggag agcatcgtca ggggctgggt cgccaacaat cccaccctca
     1441 tccagggtga caccaagatc ctggaggcgg tcgcggccgg gcagtgcgac gtggccctga
     1501 cgaacaccta ctacctcggg cgcatcctcg ccaaggacgc gaccttcccg gtggccgcct
     1561 tctgggccaa ccagcagacg accggaacgc acgtgaacgt ctcgggcgcg ggcgtcacga
     1621 cccacgcgaa gaaccgcgcg gaggcgatca ggctgatcga gttcctcacg agccccgaag
     1681 cccagcagat gttcgccgac tccaacttcg agtacccggc gaacccgcag gcgggcgtga
     1741 atccgctgat cgccgggtgg ggcaagttca agcaggacga taccaacatc gcggcggccg
     1801 gcgagttcca ggcggccgcg acgaagctgg cggaccgggc tgggtacaag taggcccgcg
     1861 gcaccgtccc tctggaccgg gctcgcggtc ggcatcacga cggtgctcgc ggtgcccatc
     1921 gcggccgtcg tcttctcgct cgcgattccc gcaggcgacg tgtggctcca tctctggcgc
     1981 acccagctcc tcgagctggt gcgacagacc gggctgctgc tggccggcgt cggtatcggc
     2041 accctgatgg tggggggcgg actcgcctgg ctcgtcgtgc accatcgctt ccccggccgc
     2101 ggcgtgctcg aatgggcgct gatcctgccg ctcgccgtgc cggcctacgt gatcggcttc
     2161 gcgttcatcg gcctgttcga gtacgcgggg ccgctgcaga gcgagctgcg ccgctggctc
     2221 ggccccggcg cgcgcctgcc cgagatccgc tcctacgcag gggtcgtggt catgatgacg
     2281 ctggtgttct atccctacgt gtacctgctg gcgcgggtgg ccttccgcga gcagggggcc
     2341 gcctccgtgg agactgcccg gatgttgggg cgctcgcgcg ccggcgcgtt cctgcacatc
     2401 actgtgccga tggcgcgccc ggctctggtg gcgggcgtgg ccctcgcgat gatggaggcc
     2461 ctcgccgact tcggcaccgt caccagcttc ggctaccgca cgctgaccga ggcgatctac
     2521 cgggtctggt acgggatgtt cgaccgcatc gcggccacgc agctcgccag cgtgctcctc
     2581 ctcttcgcgg ccggcctgct cctgctcgag cggcattcac gcggccgcgc ccgcttcgcc
     2641 cagagcggcc ggcgcgggcc ggccgtggag ccgatgcgcc tcgccgggtg gcgggccgcc
     2701 gccgcgaccg gcgcctgcct gggcgtgctc gggctcgcgt tcctgctccc ggtgggccag
     2761 ctcgcgtggt gggcggtcgt cgcgatccgc gcggggcagg tggcgcccga cttcgccacg
     2821 ctgctctggc acacctgcgc gctcgcgggt gccaccgccg cggtggcctc cctgctcgcg
     2881 gtgctgctgg cctacgcggg ccggctgcac ggcaccgcca ccgtgcgcgt ggcggcccag
     2941 ctcgcctcga tgggttatgc gctgccgggc gcggtgatcg cggtgggtgt gctcctcccg
     3001 ctcgcctggg ccgaccacgc gctggtgccc tcgctcgagc gcgcgctcgg ccggccgctc
     3061 gggctgctgc tcacaggctc cgcggccggc ctcatcctgg cctatgtggt gcgcttcctc
     3121 gccgtgggcc tgcagacggt ggacgcgagc ctcggcaagg tcccgccgag cctcgacgac
     3181 gcggcgcggg tcctgggcgt ccgggcggga gcagccctcc ggcgcgtgca cctgccgctc
     3241 atccggggcg ggctgctcac cgcggtcgtg ctcgtcttcg tcgagacgat gaaggagatg
     3301 ccggccaccc tgctgctgcg tcccatcggg cttaacacgc tggcggtgga gatctgggag
     3361 cgcaccgcgg aatcgatgtg gccggaggcg gcggtgcccg cgctgaccct ggtggcggtg
     3421 gggctgctgc cggtggtcct gctgatccgg ctcacgtcgt cgcggccgta gacccctcac
     3481 cctgcccacc cctcaccctg cccacccctc accctaccct ctcccctgag gggagaggga
     3541 tatagggtgg ggactaccct tggaggggaa aggcgaccac gtggagcacg cgggcttcgg
     3601 cgcggacccg catgccggtc gggagcaccg aggcggaggg ctgggacgag tggacccggt
     3661 cgcccgagag caggcggatg cagtacaccg tctccgagcc gcggaagtag cggcgggtga
     3721 tcgtgcccgt gccgtcggcc tgcggcacga agtggatatc gtcggggcgg atcatcacgt
     3781 cgacccggcc gccggccggg agctgatcca cgttggcgaa ggttcccacc tcggtcacga
     3841 tgccctgggc ggtcaccatg ccggggagga agtcggcggc gcccacgaag gcagccacga
     3901 accgcgtggc cggccgatga tagagctcct gcgggggcgc gatctgctcg atgcgcccgg
     3961 cgtggagcac gcccacgcga tcggccagcg tgaaggcctc ttcctggtcg tgggtgacga
     4021 acaccgcggt ggtgccgctc gtccggagaa tgcgctccac ctcgtcccgc atctgggcgc
     4081 gaagatcggc gtccaggttg gagaagggct cgtcgagcag catcagggcc ggggcggggg
     4141 ccagcgcgcg ggcgagggcc acgcgctgct gctggccgcc cgagagctcg tgcgggtagc
     4201 gcgcatcgaa gccgccgagc ccgaccaggt cgagcaccgg ctcgacgcgg ctgcgccggg
     4261 ccacgcgcgg caggccgaag gccacgttgt cggcgaccgt caggtgcggg aagagcgcgt
     4321 agtcctggaa gacgatgccg attccgcgct cctcgggggg gacggcgcgg ccgggaccgg
     4381 ccacgacctg cccgcgcagg gtgatggtac ccgcgtcggg gctctcgagc ccggcgatga
     4441 ggcgcagcgt ggtggtcttg ccgcagccgg acgggccgag cagcgcgagg atctcgccct
     4501 cggcgacccg gagcgagagt ccgtccaccg caggggggtc cgccgagccg aagcgcttgc
     4561 tgacccggtc caggagcagg aggtccacgg ctaggcgcgc ttcaagacct cgggattgac
     4621 cggcgtcggc ggggtcttgc cctccagcac ggccaggcag ttctcgaccg cgagcgtggc
     4681 catctggagc cgggtctcgt gcgaggcgct cgcgatgtgg ggagcgagca ccacgttggg
     4741 cagcgcgatg aggcccgggt gcaccgcggg ctcctcctcg aagacgtcga ggcccgcgcc
     4801 cgcgatccgg ccctcggtga gggcctggac cagcgcggcc tcgtccacca ccgggccccg
     4861 cgcggcattg atcaggtacg cggtcttctt catggtccgg agcgagtcgg cgttgatcag
     4921 gtggcgcgtc tccgggatga gcggggtgtg cagggtcacg aagtcggact cgcgcagcag
     4981 ggtggccaga tcgacgcggg tggcgttgag ctcccgctcg gtcgcgggat cggcggccac
     5041 cgcgtcctgg tagagcacgc gcatgccgaa gcccagggcg cgccgggcca tggcgcggcc
     5101 gatgcgcccg aagccgacca cgccgagcgt cttgccgtgg acgtcgccgc cctgcagcag
     5161 catgtactcc cactgccgga agcggccctc gcgcgtgtag cgatcggcct cgaccagtcg
     5221 gcgggccgtg gccatgagca gcgtccaggc gaagtcggcc gtcgtatccg tgaggacgtc
     5281 gggcgtgttg gtgaccacga cgccgcgccg cgtgcaggcc gccacgtcca cgttgttgta
     5341 gcccaccgcg acgttggaga ccacgcgcag gcccggccgg gccgcgagca gcgcctcgtc
     5401 gacgacctcg ctgatcacgc agatgaggcc ttgcttgccg gtcaggcgct cgagcagctc
     5461 ggcccgcggc atgggcgcgt ccttggcgta cgcgtccacc tcggcccggc tccgtgcgag
     5521 ctggacggcc ggctcaggca gttgacggga gatcaagacc gcgggtctac cggtccctgt
     5581 ggccattcga cctacctttc caaggtgctt attttcaagg ctatttttga tttgacatcg
     5641 tcgggagcgc tgctatatta gcactcggct tggttgagtg ctaagccctg cagcggggac
     5701 cagcccgcaa ggccaaacat cttactatcg ggaggcgtgt tgactatgaa gattcgtccg
     5761 ttgcatgacc gaattctcgt gaagcgggag gaggagaaag aggtcaagaa gggcgggatc
     5821 atcatcccgg acaccgcgaa ggagaagccc caggagggga aggtcgtcgc ggtggggggc
     5881 gggaaggtga acgaggacgg caagaagatc cctctcgacg tgaaggccgg cgacaagatc
     5941 ctcttcggca agtactcggg ctccgaggtc aagctggacg acgaggagta cctgatcctg
     6001 cgtgaagagg acgtgctcgc gatcatcgag tagccgcagc cccggagcgt agacgaacaa
     6061 cccaaacgaa cccggccggg cccacgcggg cgcggccaat tccatcgaat cggaggagag
     6121 ccagtatgcc gaagcaggtc atgttcagcg acgagggccg ggcggccctg ctgcgcgggg
     6181 tcaacatcat ggcggcggcc gtcaaggcga ccatgggccc gaagggccgc aacgtggtca
     6241 tcgacaagaa gttcggcagc ccgaccatca cgaaggacgg cgtgacggtc gccaaggaga
     6301 tcgagctcaa ggacaactac gaggacatgg gcgcgcagat ggtcaaggaa gtcgcgtcca
     6361 agacctcgga catcgcgggc gacggaacca ccaccgccac cgtgctggcg cacgccatca
     6421 ttcgtgaagg cctgaagaac gtcaccgccg gcgccaaccc gatggggctc aagcgcggca
     6481 tcgacaaggc ggtggatgcg gtggtcgacg acctcaagag gatgtccaag tccaccaagg
     6541 acaagaagga gatcgcccag gtcgcgacga tcgcctccaa caacgacaag accatcggca
     6601 gcctgatcgc cgaggccatg gagaaggtgg gcaaggacgg cgtcatcacc gtcgagga
//
LOCUS       DASUFA010000413         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_791013, whole genome shotgun sequence.
ACCESSION   DASUFA010000413 DASUFA010000000
VERSION     DASUFA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791013"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFX87_14940"
     CDS             <1..246
                     /locus_tag="VFX87_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4996277.1"
                     /translation="RVKDKNVRRSPKVALAILDPDNPYRYVQIKGRVAEMTETGADAH
                     IDSLAKKYMGVDKYPYRKPGEVRVIYKVLPERVQTNG"
     gene            250..2247
                     /locus_tag="VFX87_14945"
     CDS             250..2247
                     /locus_tag="VFX87_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX4996278.1"
                     /translation="MIALESVSKGYGGQELLRGCSWRVGRGERIGLVGPNGAGKTTLC
                     RILAGVEEPDEGRVHLDSGVTVGYLPQEVAGGIDRTVLAEALSGFDEVWELEAELERL
                     AEAMARPDADPGLTDRYGEVQHRFEALGGYRLEAEAKMILGGLGFAPAGLHRPLGEFS
                     GGWRMRAALARLLLLRPDLLLLDEPTNHLDLESLGWLENFLASYDGSVVIVSHDRYFL
                     NRMVTSIAELAGGEVVVYPGDYDHFLVEREARRALVEARARNQAKRIEEIERFIDRFR
                     YQATKARQVQSRIKMLEKVERIEVDGDARKIHFKFPQPPRTGRIVIRLDGVRKAYGDN
                     VVYAGVDFLAERAERIALVGVNGAGKSTLLKMLAGVLPPDAGQRVLGSHVEVHYYAQH
                     QLDALDPAHTVLEEIEAAAPDATHTRLRTILGSFLFGGDAVDKRVAVLSGGEKARLAL
                     AKMLVRPAALLCMDEPTNHLDLASKEVLEEALAGFTGTIVFISHDRYFINRIGTRVVE
                     VQGGRLTDYLGNYDDYLAASAARTSAAPASPPAPSPLAPAPARHLSPAARLGAARPAA
                     AREPSPPRKEGTSDKPGRRALEREIKAIRGRLEAIEKQIAEMETRLADIGLALADPDL
                     YRDGNRAREISESRRQAEEQVAWLMKEWEELSERLTAVEGG"
     gene            2259..2576
                     /locus_tag="VFX87_14950"
     CDS             2259..2576
                     /locus_tag="VFX87_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX4996279.1"
                     /translation="MPEPRFVPVVGEAELRREKARARELRQSAWWKRRIADGICHYCG
                     RRVGHRGLTADHVVPLIRGGRSSRGNMVPACKDCNNRKQSLLPWEWEDYLKGQGRGGA
                     EPD"
BASE COUNT          405 a          900 c          958 g          345 t
ORIGIN      
        1 cgggtgaagg acaagaacgt gcggcgcagc cccaaggtgg cgctggccat cctggacccg
       61 gacaatccgt accggtacgt gcagatcaag ggccgggtgg cggagatgac cgagaccggg
      121 gcggacgccc atatcgattc gctcgccaag aagtacatgg gcgtggacaa gtatccctac
      181 cggaagccgg gcgaggtgcg agtgatctac aaggtcctcc ccgagcgcgt gcagaccaac
      241 ggctaggtcg tgatcgcgct cgaaagcgtc tccaagggct acggcggaca ggagctcctg
      301 cgtggatgct cgtggcgcgt gggccgcggc gagcgcatcg ggctcgtcgg ccccaacggg
      361 gcggggaaga ccacgctctg ccgcatcctg gccggagtcg aggagcccga cgaggggcgc
      421 gtgcacctcg acagcggcgt caccgtcggc tacttgcccc aggaggtggc gggcgggatc
      481 gaccgcaccg tgctcgcgga ggcactgtcc ggcttcgacg aggtctggga gctggaggcg
      541 gagctggagc ggctggccga ggcgatggct cgccccgacg cggaccccgg gctcaccgac
      601 cgctacggcg aggtccagca ccgcttcgag gcgctcggcg gctaccggct cgaggccgag
      661 gccaagatga tcctgggcgg actcggcttc gcgcccgccg ggctccaccg gccgctgggc
      721 gagttctcgg gtggctggcg gatgcgggcg gccctggcgc ggctcctgct gctgcgcccg
      781 gacctgctgc tcctggacga gcccaccaac cacctcgatc tcgagtcgct gggctggctc
      841 gagaacttcc tcgcctccta tgacggcagc gtggtgatcg tgtcccacga ccgctacttc
      901 ctcaaccgca tggtcacctc catcgccgag ctcgcgggcg gggaggtggt ggtgtatccg
      961 ggcgactacg atcacttcct ggtggagcgc gaggcgcggc gggcgctcgt cgaggcccgg
     1021 gcgcgcaatc aggccaagcg catcgaggag atcgagcgct tcatcgaccg gttccgctac
     1081 caggccacca aggcccggca ggtgcagagc cggatcaaga tgctggagaa ggtcgagcgg
     1141 atcgaggtgg acggcgacgc gcggaagatc cacttcaagt ttccccagcc gccccgcacc
     1201 ggccggatcg tgatccggct ggacggggtg cgcaaggcct acggcgacaa cgtggtatac
     1261 gcgggggtgg acttcctcgc cgagcgcgcg gagcgcatcg ccctggtggg cgtcaacggc
     1321 gccggcaagt ccaccctgct caagatgctg gccggcgtgc tgccgcccga cgcgggccag
     1381 cgcgtgctgg gctcccacgt cgaggtgcac tactacgcgc agcatcagct cgacgccctc
     1441 gatcccgcgc ataccgtgct ggaggagatc gaggcggccg cccccgacgc cacccacacc
     1501 cgcctgcgga ccattctcgg ctcgttcctc ttcggcgggg acgcggtgga caagcgcgtg
     1561 gcggtgctct ccgggggaga aaaggcgcgc ctcgcgctgg ccaagatgct ggtgcgcccg
     1621 gccgcgctgc tctgcatgga tgagccgacc aaccacctcg acctcgcctc caaggaagtg
     1681 ctggaagagg ccctggccgg cttcaccggg accatcgtct tcatctccca cgaccgctac
     1741 ttcatcaacc gcatcggcac gcgcgtggtc gaggtgcagg ggggacggct caccgactat
     1801 ctcggcaact acgatgacta tctcgccgcc agcgcggcgc ggacgtcggc ggcgccggcc
     1861 tccccgcccg cgccgtcccc cctcgccccg gcgcccgccc gccacttgtc gccggccgcc
     1921 cggctcgggg ccgcgcgacc cgcggccgcc cgcgagccat ccccgccgcg aaaggaagga
     1981 acgtccgaca agccgggacg ccgggcgctg gagcgggaga tcaaggccat ccggggccgg
     2041 ctcgaggcca tcgagaagca gatcgccgag atggagacac ggctggccga catcgggctg
     2101 gccctggccg atccggacct ctaccgggac ggcaaccgcg cgcgcgagat ctcggaatcg
     2161 cgacggcagg cggaggagca ggtggcctgg ctgatgaagg aatgggagga gctgtccgag
     2221 cggctgaccg cggtcgaggg cgggtgaggg aggtcccgat gccggagccg cgcttcgtcc
     2281 ccgtggtggg cgaggccgag ctgcgccgcg agaaggcccg cgcgcgcgag ctgcgccaga
     2341 gcgcctggtg gaagcgccgc atcgccgacg ggatctgcca ctactgcggg cgacgggtgg
     2401 gccatcgcgg gctgaccgcc gatcacgtgg tgccgctgat ccggggcggc cgctcgtcgc
     2461 gcggcaacat ggtgcccgcc tgcaaggatt gcaacaaccg caagcaatcg ctgctgccct
     2521 gggagtggga ggactacctc aagggacagg gccgcggcgg cgcggaaccg gattgagggt
     2581 gccgcgccgc gcggcgctcg cgctcctc
//
LOCUS       DASUFA010000414        19158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_534810, whole genome shotgun sequence.
ACCESSION   DASUFA010000414 DASUFA010000000
VERSION     DASUFA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 19158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19158
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534810"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..742)
                     /locus_tag="VFX87_14955"
     CDS             complement(173..742)
                     /locus_tag="VFX87_14955"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX4996280.1"
                     /translation="MATTDALWAAWEGPGFEHLELRQEPGAVRADSLIIAVEDKGRPY
                     RARYVVECDADWKVARARIEVLEDPGRVLAVRADGQGHWTAATGEALALDGCVDIDVY
                     PSPFTNTLPISRLAEMPVGRPVALDVAWVVLPELTIQVARQEYTLLERGADGARWRFR
                     GLDSDFVAELDVDGNGLVRDYPGIARRVL"
     gene            930..1550
                     /locus_tag="VFX87_14960"
     CDS             930..1550
                     /locus_tag="VFX87_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX4996281.1"
                     /translation="MFDLIADPEVIGAHLSEDSLLAPLVAARPGLRVAGAWDGFELAV
                     RALLGQQITVADAATPAGRLVAAYGKPLAGGGEALSHVFPGPERLADADLSSLGLTRA
                     RAPALGSLARAVVADPQIFGPRQSLDAAVLKLRALPGIGEWTAQYIAMREPDVFPAAD
                     IGLMRADGRSEGAPPEPRRAARARRALATLARVCGAAPVGVPGDEL"
     gene            1568..2854
                     /gene="larA"
                     /locus_tag="VFX87_14965"
     CDS             1568..2854
                     /gene="larA"
                     /locus_tag="VFX87_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX4996282.1"
                     /translation="MTIDLLYGRSTLPVTPPPGCVPTVIEKRAIPALAEPLAAVERAL
                     AVPVGSPALRELARGKRSACILICDITRPVPNRLFLAPLIRTLLDAGVPRAGITVLVA
                     TGLHRPNEGPELAELVGDPWVLETVTVANHDARADEDHVLLGRTPARGTVVRLDRRLV
                     EADLKIATGLVEPHFMAGWSGGRKVIAPGVAHAETITTFHNAAFMAHPRAANCVLDGN
                     PLHEEQLAIVGMLGGALALNTVIDDRRRPAFVNFGEVVQSHLQAVDFAGGYARVPVGR
                     RFQTVVTSAAGYPLDRTYYQTVKGMVGPLDILAPGSDLIVASACSDGMGSKHYVEAQR
                     RLVDLGAAGFASSIAGKPHADIDEWQTQMQLKPMRVGRVRLYTDGLEAHDAALTGVER
                     VADLTAAIADSMARHRDPRVAFVPEGPYVVPVHEEA"
     gene            2854..4440
                     /locus_tag="VFX87_14970"
     CDS             2854..4440
                     /locus_tag="VFX87_14970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4996283.1"
                     /translation="MFDAVIVGAGFAGLYMLHRLRGLGLTARVYEAGKGVGGTWYWNR
                     YPGARCDVESMDYSYSFSDELQQEWRWTERYAAQPEILTYANHVADRFDLRRDIQFET
                     RVTAAAFDEATRRWTVETDRGDRVSARFCIMATGCLSDAQVPDIEGRESFAGTWYHTG
                     RWPHEGVDFTGLRVGIIGTGSSAIQSIPIIAKQAAHLFVFQRTPNYSMPAQNAPLDPD
                     YEKRVKDSYADFRRQARESRVGFVVERSGDSALAVPEEERRREYEKRWSRGGLGFSAA
                     YTDLLTSKEANDTAAEFFRAKIRDIVRDPEVARALTPTDYPLGTKRLCVDTGYYDTFN
                     RDNVTLVDLRKTPIEAITAKGVRTSEAEYAVDALVFATGFDAMTGALLNIDIRGRGGR
                     RIQEAWAAGPRTYLGLSIAGFPNLFTITGPGSPSVLSNMIVSIEQHVDWIADCLTYLR
                     RHGHASIEATDEAQEAWVRHVNEVGHMTLYPLAKSWYMGANVPGKPRVFMPYIGGVGV
                     YRQKCDEVAAKGYEGFALGA"
     gene            complement(4451..5707)
                     /locus_tag="VFX87_14975"
     CDS             complement(4451..5707)
                     /locus_tag="VFX87_14975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996284.1"
                     /translation="MRDQKVFYGWWVVGAFSVTTFMSTGVRHAVGPFLKPIVADLGLD
                     RASFSAVIALSLFLYGVFMPLAGMALNRFSVRVVTSAGTLLLVASLVLTAMVRNLWEF
                     TAVYGVLVPLGLAGTGPVIASGVVARWFSRRRGTALSVLGSASMTGMSLLVPAVTWLV
                     LTAGWRVTYVVIAGLVLAVSLPLCLWVMRDSPESVGLTVDGAPPAAGAATGSVERVTA
                     GEALRTLAFWQLAGSFFTCGFSMTLLSAHGIPMLTDHGYSPMFASWALGVLGGSSIGF
                     TVMLGALSDRFGRRPVLASIYAGRVLIFAGFFLIRDNPTAILAVAVLGGITMAGTGSM
                     TSALTADIYGRFSVSSVFGLIFLVHQTGAALGSWLAGALFETTGGYGPAYAVACVFLV
                     TASVVALNIDRDARRIWRASPASASS"
     gene            complement(5730..6572)
                     /locus_tag="VFX87_14980"
     CDS             complement(5730..6572)
                     /locus_tag="VFX87_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4996285.1"
                     /translation="MSSNRQPVAVVVGATSKWQADGRNTKLAHGKALDDRDLPVGVRW
                     GVGGAIAQKFAKEGFFVVLTTRKAANAAALEQAIREQGGASTIVEMDLVSDASISKAF
                     TTIREKAGDPEVLVYNAGYLEGRDLPPDKELLEHVPVEILETAQHIASRGPFLVAKEV
                     LPAMRKRGAGSFLISNNSSSLRGRKRLTGQSLYYPRVMMRTLAQVLTEEYSEHGVHVA
                     NVVIDGLIDSPGTRALPRAQKQPEIVMNPVKIAEAFYYLHTQDRSCWTHELQLTPFST
                     KPSF"
     gene            complement(6569..7375)
                     /locus_tag="VFX87_14985"
     CDS             complement(6569..7375)
                     /locus_tag="VFX87_14985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4996286.1"
                     /translation="MTAPRMAVRRLGRGPDLVLFHGGMGSWKHWIRNVEALGERFTVH
                     ALDHPSYGDSAPVPRETTGPQYLDLVHELLLEALPGAAPLRLAGFSFGAAIAANMARR
                     LGPRVSHLGLISPGGFPMRRFGERPIRSYKEAGGDDRLFREVCRHNLLVNMLSDPASV
                     SEETVDIQVDLVRRTRFDSRKVSGGGTLLGDLAGVARRGCRIRLLWGERDDSAFRPAK
                     LLIGEIREAVPGLDVHRIPKAGHWSAYENAPEVNRLLLEFLGGATEEKLQ"
     gene            complement(7372..8271)
                     /locus_tag="VFX87_14990"
     CDS             complement(7372..8271)
                     /locus_tag="VFX87_14990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4996287.1"
                     /translation="MQVGVWLPNCRHLATPDIIRRTAVRAEQLGYDSVWVSDHVVVPD
                     ANVDRIGAGIYDPLVTLAVAAGATSRVRLGTTVLIVPYRNPVVTAKMVSSLDALSGGR
                     VVLGVGAGWLAQESAALGVPFAERGAMTDEYLAAMRELWTSPAPSFAGKYTRFSGLKF
                     EPKPVQKLHPPIWVGGHGRASLRRAAEIGAAWHPINRSVEELRDGRAELARLCQARGR
                     AVPPPIALRNDLCVLRPGRPAGTSVRGGSVLVGEPSALIERIIELRAVGVEHLVLEFL
                     AADGSELDEQMALFAERIRPKLA"
     gene            complement(8271..9209)
                     /locus_tag="VFX87_14995"
     CDS             complement(8271..9209)
                     /locus_tag="VFX87_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4996288.1"
                     /translation="MATHKPTFKQITSGLRFPEGPVAMPDGSVILVEIERRTLSRVTP
                     DGKIHVIATLGGGPNGAAVGPGGKIYVTNNGGLKFVDRPGKLFPVAQADDYTTGSIQI
                     VDPETGRFDTLYDRCDGQRLRGPNDLVFDGAGGFWFTDLGKTRERDSDRGVVYYAKAD
                     GSSIKEAIFPLERPNGIGLSPDEKTLYVVETPTARCWAFKLSGPGQIESANGPYRGEK
                     GTVIAGLGGYQMFDSLAVDGLGHVCVATLITGAVSDIWPDGSRVDQYMLPDMMVTNVC
                     FGGQDLRTAYATLSMGGTLVSFEWPRPGLPLRHLNR"
     gene            complement(9209..10063)
                     /locus_tag="VFX87_15000"
     CDS             complement(9209..10063)
                     /locus_tag="VFX87_15000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4996289.1"
                     /translation="MDAGAAIFFTDYSIGPAALGRALEERGFGSLWAPEHSHIPLSRA
                     SAFPQGGDLPKKYYDVMDPFVTLAAAAAVTTTLQVATGVCLVVQRDPIQTAKAVASLD
                     QVSGGRFLFGIGAGWNAEEMADHGTEFKSRFEVMRERVEAMRAIWTKSKPEYTGEFVK
                     FPPMMTWPKPVQKPHPPVLVGGAFPYGARRAIAYGDGWVPHARRPAYGDVLAKLPEFR
                     KMVVEAGRDPATLPITVFGVAEDGDLIKRYRDAGVARLIFSLPPAKADEVLPVLDRCA
                     ALMRQAQS"
     gene            10166..11128
                     /locus_tag="VFX87_15005"
     CDS             10166..11128
                     /locus_tag="VFX87_15005"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996290.1"
                     /translation="MATKLMARLALVAMTGALLFLPTGATAQEVIRIGCPTKTYFPTI
                     LATVAKEKGLFEKEGLRPEITIYRGGGETFEALAANSADLGPVAVPLVATSRNRGVLT
                     KIVGGNGDEWSGWILGVRTASPIKSAKELDGKKVGITSAGSGTDTLALWGQSEHKVTF
                     QRVPVGGGGLVPNLLNDNLAAAVIYSPLSYQVVSEGKVRVLMDFATAMPPNLIGGWTA
                     TEKNLTERRPFIQKGLNALYGALEYMRNNPDYAIKTIATNNELPIEIAKMEYEKTFLR
                     LSRDGKMTLASVQKAMDLAAASGVKDMAPAADVFMNMPISPTKP"
     gene            11139..11894
                     /locus_tag="VFX87_15010"
     CDS             11139..11894
                     /locus_tag="VFX87_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4996291.1"
                     /translation="MRRHEHVIVAAAVLGLFGLWEALVRLGRLDPSLAPAPSTITGAL
                     VRLLERPEVLASLAVTAWEAVAAFLIAVPVGLLIGFTLAEIPGLGGLLRPLVNFLFGV
                     PKSIFLPVFILMFGVSIPQKIAFGVFTTVFVLIMGGIAAVQSVPHELVTVSRVYGASR
                     LQIIREIYLPAMAPILLESARLGMVFNITAVLLCEIYGARDGIGYRIAAWGENLQMPQ
                     LYAALVIVATAAVAVNEALRLVETRLGAWRQRS"
     gene            11891..12664
                     /locus_tag="VFX87_15015"
     CDS             11891..12664
                     /locus_tag="VFX87_15015"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996292.1"
                     /translation="MTRPLVAIRGVSQRFGVDGASVAALEAIDLDIPDGQFVSVVGPS
                     GCGKSTLLSLVAGLSTPSSGTVLCDGEPITAPMPRKVGVIFQEANLLPWLSAIDNVAF
                     PLKLRRVPKAERLEAAARMLELTGLAGFETRLPHQLSGGMKQRVAIARGLVQNPAVLL
                     MDEPFASLDEQTRMVLGDELLRIWSETRKTVLFVTHSLNEAVYLADRVVVLSARPGRV
                     VDDVDVDLPRPRSFAMTSSERFGVLKDRIWQHIKHAERR"
     gene            12661..13404
                     /locus_tag="VFX87_15020"
     CDS             12661..13404
                     /locus_tag="VFX87_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996293.1"
                     /translation="MTRLRWGQAALYLAILAAWELLPRAGVIPRLFVPPLSEALGALV
                     ADRREYLGSLPTTFGEILVSYVIVCGGGILLGQLIGASAAARRILLPVLRSAYAVPLV
                     VLYPVMMVWFGLGSESKIAFASIYAFIPTLLTAVAGVAALSPALAETARAFGATRAQQ
                     ILYVSLPASLPSIVAALRLGGALVIVGVIVAQMLGAADGLGYLITRHRTLLNSPGVYA
                     GIVLVLGITGMHEILLRRLEHKVAAYRQS"
     gene            13424..14455
                     /locus_tag="VFX87_15025"
     CDS             13424..14455
                     /locus_tag="VFX87_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HEX4996294.1"
                     /translation="MVQQALTTTVVGSYPQPDWLVDRALLSKGVPRVRLRSIWRVPDD
                     LLEQAQDDATVVAIRDMERAGVDIISDGEIRRESYSNRFATALDGVDVNQPAVITDRY
                     GNQTQVPRVVGPIRRVRPIELRDMQFLRRNTDRPAKITLPGPFTMAQQVRNEHYRDLE
                     ELAMDYAEAVNAEAHDLQAAGADVIQLDEPWLRNDPEAAKRYAVPAINRALAGLTVPT
                     ALHLCFGYAAVVKQQKPTGYSFLPQLAGTIAQQISIEAAQPRLDLGVLSDLAGKTIIL
                     GVIDLADPAVDTIEQVTSRIRAGLARVPAERLILAPDCGMKYLPRHTAFNKLCMLTAS
                     ARAVRAQLA"
     gene            14465..15295
                     /locus_tag="VFX87_15030"
     CDS             14465..15295
                     /locus_tag="VFX87_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4996295.1"
                     /translation="MAVLLLDDPLPGVRRLTLNRPEKRNALSNELRGAILDALKAADA
                     DPAVRVSVLRGAGPAFSSGYDLSADNSVNQPYYSPGGPGQWARHVVDGWFTIWDLAKP
                     VIAQVHGYCLAGGSELAACCDLVYVAEDARIGYPPTRLMSPPDNQFHPWLMGMRTAME
                     MFLTGDAITGTEAAARGFANRAVAAADLERTVLGVAERIALVPTELTQINKRSVHRAM
                     EIMGLRAAIRAGTELQALAFTTQASLQYRAQFKRDGESVRDLLSQRDAAFGDYRERER
                     "
     gene            complement(15392..16267)
                     /locus_tag="VFX87_15035"
     CDS             complement(15392..16267)
                     /locus_tag="VFX87_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4996296.1"
                     /translation="MLTRIDHVMICVPDLAKGIEVYTRLGFTVYPGGSHTGRPTHNAI
                     AFHDEDYLELLSIRDRRAVVPGSPDASLAEYLDQGGGFRIVALQSDDLAADVAAMRRR
                     GVDVGEIRDGGRRTPEGQELRWRAAFLGPSSALPIFLIQHLTPLAERKARAVQLGSHP
                     NGVMRVVRVYIAVTDVAAEARTYAQVLGLPVPAIQRGAVIRADMAVFDLGPTGLTLAQ
                     PAEPGPAAEALARRGPGPFQVLHRVRSLDAVARVLAERGVPTPARGVRNTGEQAMLVG
                     PEHACGAYIGFVGVA"
     gene            complement(16280..17533)
                     /locus_tag="VFX87_15040"
     CDS             complement(16280..17533)
                     /locus_tag="VFX87_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX4996297.1"
                     /translation="MRPRHALSIWLLGLALLTGAVAPAPPAAAQAKKPIKVGFLAPLT
                     GGAAQVGRDTVNGFEMYLEEAGHQIAGRKVELIVEDTAGNPGTAITKFRKFVESDQVD
                     MVVGEVFAHIGYALAPKADEFKMPTIFPVIAADDLTQRKPSKWVVRLGWTGSQPSHPF
                     GEYAAKTLGYKKVAVFGSDYAFGYEVVGGFQRSFEEAGGQIIQKLWAPLGTNDLAPYL
                     SQIKREADAVFVILAVPSSLRFPAQYRDAGLYGKIPVIGGAVIVDESILPSFGDETLG
                     YVTPLMFSAALDTPANRKFVADYRKKYGKVPSYFAETCYTSARWISEAAKLVGGNVEE
                     REKFMAAFRKVEIPDAPRGPVKLDQWGNPIQNIYVRKVERKGGELQNTVVHTFPAVSQ
                     FWTYKPEEFLKQPVYNRDYPPCKGC"
     gene            complement(17536..18405)
                     /locus_tag="VFX87_15045"
     CDS             complement(17536..18405)
                     /locus_tag="VFX87_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4996298.1"
                     /translation="MAPIARARTGRGAPRKPRARRARFTRLRPGLRSLGRGLKRAGLA
                     LLAAPLAVRIVGFAMALVIVWSAANWTYQVIRKPSELFFPVSGTLSKAPAETWRSYAP
                     LFRRHATATITPELLAALAQVEGSGDPVARTYWRWQVAWHPFEVYRPASSAVGMYQIT
                     DPTFREAKRFCVRDHVVAEDQCWFRSLYTRVLPGHAVELTSAMLDRGVARTLERRRIV
                     TATPRQKQDLAALIHLCGEGAGDAHARRGFRLTPGQQCGDHDVARYLAQVNGMKRLFA
                     RLAGDDPSISARR"
     gene            complement(18454..>19158)
                     /locus_tag="VFX87_15050"
     CDS             complement(18454..>19158)
                     /locus_tag="VFX87_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4996299.1"
                     /translation="LHGMGANAGVWERLLPLIEGAWRGRWLAPDLRGHGRSVREGPYG
                     FGMHAADVAGLIETEAAGPVTLVGHSFGGAVAALVASGWFGPRVRDVAAFGVKIEWSL
                     DEMAKAREMAGRPAPVFTTRDEATVRYLRISGLAGLAPASSATAAAGVTGTDGRFQVA
                     MDPRAYGAVGPSVEGLLRLAETPLRLAAGDRDPMVTLEQMRRIDPAARVLEGVGHNAH
                     WEAPEQVWDFIAGGPR"
BASE COUNT         2822 a         6957 c         6696 g         2683 t
ORIGIN      
        1 tgcctccaga gggaggagag atgccccgcc cccttcccct caccccagag gaaggagaga
       61 tgccggcccg cttccccttc cttcagaaga gagggggatg cccggtctct ttttcccctg
      121 tccactcagg ggagagggca gggtgagggg tggtctaggg gtggtctccg ggtcagagca
      181 cccgccgcgc aatccccggg tagtcccgca cgagcccgtt gccgtccacg tcgagctccg
      241 ccacgaaatc cgaatcgagc ccccggaacc gccaccgggc cccgtcggcc ccacgctcga
      301 gcagcgtgta ctcctgacgc gccacctgga tggtgagctc gggcagcacc acccacgcga
      361 cgtccagggc gacgggccgg ccgaccggca tctcggccag ccgcgagatc ggcagcgtgt
      421 tggtgaacgg tgagggatag acgtcgatgt ccacgcagcc gtcgagggcc agggcctcgc
      481 cggtcgcggc agtccagtgg ccctgcccgt cggcgcgcac cgcgaggacg cgtccggggt
      541 cctcgagcac ctcgatccgc gcccgtgcca ccttccaatc cgcatcgcac tccacgacgt
      601 agcgcgcccg atacggccgg cccttgtcct cgaccgcgat gatcaggctg tccgcgcgca
      661 ccgcgccggg ctcctgccgg agctcgaggt gctcgaatcc ggggccctcc cacgccgccc
      721 agagcgcatc ggtcgtggcc atggcaagat catcccagac cgggcaaccg gcccctggcg
      781 ggatccggac ccggttggtc gggtccgaaa gtcgcgagac ggggaggcac gcgtgcgcga
      841 agatcggccc atggacctgg acgcggaggc ctgctaccgc gccatcgccg ctctccgcgc
      901 tgcccaccat cgtcgcgcgg ctccggcgcg tgttcgacct gatcgccgat ccggaggtga
      961 tcggcgcgca cctgtccgag gattcgctcc tggctccgct cgtggcggcg cggccgggcc
     1021 tgcgcgtggc tggcgcgtgg gacgggttcg agctcgcggt gcgcgccctg ctcggtcagc
     1081 agatcacggt ggccgacgcg gccacccccg cgggccggct ggtggcggcc tatggcaagc
     1141 cgctggccgg cggcggcgag gcgctgagcc acgtcttccc aggccccgag cggctggccg
     1201 acgccgacct ctcgagcctc ggcctgaccc gggcgcgcgc ccccgccctc ggctcgctgg
     1261 cgcgcgcggt ggtggccgat ccgcagatct ttggcccacg gcaaagcctc gacgcggcgg
     1321 tgctgaagct gcgcgcgctc cccggtatcg gcgagtggac ggcgcagtac atcgcgatgc
     1381 gcgagcccga cgtgttcccg gccgccgata tcggcttgat gcgcgccgat ggccgatcgg
     1441 aaggggcgcc gcccgagccc cggcgagctg ctcgcgcgcg ccgagcgctg gcgaccctgg
     1501 cgcgcgtatg cggcgcagca cctgtgggcg tcccagggga tgagctatga tcgcggggag
     1561 gcccccggtg acgatcgatc tcctttacgg ccgctcgacc ctccccgtca cgccgccccc
     1621 cggctgcgtg ccgacagtga tcgagaagcg cgcgattccg gcgctggccg agccactggc
     1681 cgcggtggag cgcgccctcg ccgtgcccgt cggcagcccg gcgctgcgtg agctcgcgcg
     1741 aggcaagcgc tccgcctgca tcctcatctg cgacatcacc cggccggtgc cgaaccgcct
     1801 cttcctggcg ccgctgatcc gcaccctgct ggacgcgggc gtgccgcgcg cgggcatcac
     1861 cgtgctagtc gcgacggggc tccatcgccc gaacgagggc ccggagctgg ccgagctggt
     1921 gggcgacccg tgggtgctcg agacggtgac cgtcgcgaac cacgacgccc gggcggacga
     1981 ggatcacgtg ctgctcggcc ggactccggc gcgcggcacc gtggtgcggc tcgaccggcg
     2041 cctggtcgag gccgacctca agatcgccac cggcctggtg gagccgcact tcatggccgg
     2101 ctggtcgggc gggcgcaagg tcatcgcgcc cggcgtcgcg cacgctgaga ccatcaccac
     2161 ctttcacaac gcggccttca tggcgcaccc gcgcgcggcc aactgcgtgc tcgacggcaa
     2221 cccgctgcac gaggagcagc tcgccatcgt cgggatgctg ggcggcgcgc tggcgctcaa
     2281 caccgtgatc gacgaccggc ggcggccggc cttcgtcaac ttcggcgagg tggtgcagag
     2341 ccacctccag gcggtggact tcgccggcgg ctacgcgcgc gtgccggtgg gccgccggtt
     2401 ccagaccgtg gtcaccagcg cggccggcta cccgctcgac cggacgtact accagaccgt
     2461 caagggcatg gtgggcccgc tcgacatcct ggcgccgggc agcgatctga tcgtcgcctc
     2521 cgcgtgctcg gacggcatgg gatccaagca ctatgtggag gcgcagcgcc gcctggtaga
     2581 cctgggcgcc gccgggttcg cgtcgagcat cgcgggcaag ccgcacgccg acatcgacga
     2641 gtggcagacg cagatgcagc tcaagcccat gcgggtcggc cgcgtgcggc tctacacgga
     2701 cggcctggag gcgcacgatg cggcgctgac cggcgtggag cgggtggccg atctgacggc
     2761 cgcgatcgcg gacagcatgg cgcggcaccg ggacccgcgg gtggccttcg tccccgaggg
     2821 accctacgtg gtgccggtgc acgaggaggc gtgatgttcg acgcggtgat cgtcggcgcg
     2881 gggttcgccg ggctctacat gctccaccgt ctgcgcgggc tcggcctcac cgcgcgtgtc
     2941 tacgaggcgg gcaagggcgt cggcggcacc tggtactgga accgctaccc cggcgcgcgc
     3001 tgcgacgtcg agagcatgga ctactcgtac tcgttctcgg acgagctgca gcaggagtgg
     3061 cgctggaccg agcgctacgc ggcccagccg gagatcctga cgtacgccaa ccacgtggcc
     3121 gaccggttcg acctgcgccg ggatatccag ttcgagacac gcgtcaccgc cgccgccttc
     3181 gacgaggcga cccgccggtg gaccgtcgag accgaccgcg gcgaccgcgt gtcggcgcgc
     3241 ttctgcatca tggccaccgg ctgcctctcg gacgcgcagg tccccgacat cgagggccgc
     3301 gagtccttcg cgggcacgtg gtaccacacc ggcaggtggc cccacgaggg cgtggacttc
     3361 accggcctgc gcgtcggcat catcggaacc gggtcgtccg cgatccagtc catcccgatc
     3421 atcgcgaagc aggccgcgca tctcttcgtc ttccagcgca cgcccaacta cagcatgccc
     3481 gcccagaacg cgccgctgga tcccgactac gagaagcgcg tgaaggacag ctacgccgac
     3541 ttccggcgcc aggcccgcga gtcgcgggtc ggcttcgtgg tcgagcgcag cggggactcc
     3601 gcgctcgcgg tgcccgagga ggagcgtcgg cgggaatacg agaagcgctg gagccggggc
     3661 gggctcgggt tctcggccgc gtacaccgat ctgctcacca gcaaggaagc gaacgacacc
     3721 gcggccgagt tcttccgggc aaagatccgg gacatcgtcc gcgacccgga ggtcgcgcgg
     3781 gccctgacgc ccacggacta tccgctcggc accaagcgcc tctgcgtgga caccggctac
     3841 tacgacacgt tcaaccggga caacgtcacg ctggtcgacc tgcgcaagac gccgatcgag
     3901 gccatcactg cgaagggcgt gcggacgagc gaggccgagt acgcggtgga cgccctcgtc
     3961 ttcgccacgg gcttcgacgc gatgaccggc gcgctgctca acatcgacat ccgcgggcgg
     4021 ggcggccggc gcatccagga ggcgtgggcc gcggggccgc ggacctatct cggcctctcg
     4081 atcgccggct tcccgaacct cttcacgatc accggcccgg gcagcccgtc cgtgctcagc
     4141 aacatgatcg tctccatcga gcagcacgtg gactggatcg ccgactgtct cacctacctg
     4201 cgccggcacg ggcacgcgag catcgaggcg accgacgagg cgcaggaggc gtgggtccgg
     4261 cacgtcaacg aggtggggca catgacgctc tacccgctgg ccaagtcctg gtacatgggc
     4321 gcgaacgtcc ccggcaagcc ccgcgtcttc atgccgtaca tcggcggcgt cggcgtctac
     4381 cggcagaagt gcgacgaggt cgcggcgaag ggctacgagg gattcgcgct gggcgcgtga
     4441 ggcccgcgcc tcagctcgag gcggaggcgg gggaggcgcg ccagatgcgg cgcgcgtcgc
     4501 ggtcgatgtt gagcgcgacc accgacgcgg tcacgaggaa cacgcaggcc accgcgtagg
     4561 cgggcccgta gccgccggtc gtctcgaaca gcgcgccggc cagccacgat ccgagggcgg
     4621 cgccggtctg gtgcaccagg aagatcagcc cgaacaccga gctgaccgag aagcgcccgt
     4681 agatgtcggc ggtgagcgcc gaggtcatcg agccggtgcc cgccatggtg atcccgccca
     4741 ggaccgccac cgccaggatc gcggtcgggt tgtcgcggat caggaagaac ccggcgaaga
     4801 tcagcacccg gcccgcgtag atggaggcca gcaccggccg gcggccgaac cggtcggaga
     4861 gcgcgccgag catcacggtg aagccgatgc tcgagccgcc cagcacgccc agcgcccacg
     4921 acgcgaacat cggcgagtac ccgtgatcgg tgagcatcgg gatcccgtgc gcggagagca
     4981 gggtcatcga gaagccgcag gtgaagaagg agcccgcgag ctgccagaag gcgagcgtgc
     5041 ggagcgcctc gcccgcggtg acgcgctcga ccgacccggt ggccgctccg gccgccggcg
     5101 gcgcgccgtc gacggtgagc ccgaccgact ccggggagtc gcgcatcacc cagaggcaga
     5161 gcggcagcga gaccgccagg accaggcccg cgatgaccac gtaggtgacg cgccatccgg
     5221 cggtgaggac cagccacgtc accgcgggca cgagcaggct catcccggtc atcgaggcgc
     5281 tgccgagcac cgagagcgcg gtgccgcgcc gccggctgaa ccagcgcgcc accaccccgg
     5341 aggcgatcac cgggccggtc cccgccagcc cgagcggcac cagcaccccg tagaccgcgg
     5401 tgaactccca gaggttgcgc accatcgcgg tcagcaccag ggaggcgacc agcaggagcg
     5461 tgccggccga ggtgaccacc cgcacgctga agcggttcag cgccatgccg gccagcggca
     5521 tgaagacccc gtagaggaac aggctcagcg cgatcaccgc cgagaagctg gcgcggtcga
     5581 ggccgaggtc ggccacgatc ggcttcagga agggcccgac cgcgtggcgc acacccgtcg
     5641 acatgaaggt ggtgaccgag aacgccccca cgacccacca gccgtagaag accttctggt
     5701 cgcgcatgtc tactcgacta tatcgcagcc tagaagctcg gcttggtcga aaacggcgtg
     5761 agctggagct cgtgcgtcca gcaggaccgg tcctgggtgt ggaggtagta gaacgcctcc
     5821 gcgatcttca cgggattcat gacgatctcc ggctgcttct gggccctcgg cagggctctc
     5881 gtccccgggg agtcgatgag gccgtcgatc accacgttgg ccacgtgcac gccgtgctcg
     5941 gaatactcct cggtgagcac ctgcgccagc gtgcgcatca tcacgcgcgg atagtagagc
     6001 gactgtccgg tcaggcgctt gcgccccctc aaggacgacg aattgttcga gatgagaaac
     6061 gagccggcgc cgcgcttgcg catggcgggc agcacctcct tggcgaccag gaacggcccg
     6121 cggctcgcga tgtgctgcgc ggtctccaag atctcgacgg gaacgtgctc gagcagctcc
     6181 ttgtcgggcg gcaggtcgcg cccctcgagg tagcccgcgt tgtagaccag gacctccgga
     6241 tcgcctgctt tctctcggat agtcgtgaac gccttcgaga tggatgcgtc cgacaccagg
     6301 tccatctcga cgatcgtgct cgcgccgccc tgctcccgga tggcctgctc gagcgccgcc
     6361 gcattggcgg ccttccgggt cgtcagcacg acgaagaacc cttccttggc gaacttctgg
     6421 gcgatggcgc cgccgacgcc ccagcgcacg ccgacgggca gatcgcggtc gtcgagcgcc
     6481 ttgccgtggg ccagcttggt attgcggcca tcggcctgcc acttggaggt cgcgcccacc
     6541 acgacggcga ccggctgacg attcgagctc attgtaattt ctcctctgtg gcgccgccga
     6601 ggaactccag cagcagccgg ttgacctcgg gcgcgttctc gtacgcggac cagtggccgg
     6661 ccttcgggat gcgatgcacg tcgagcccgg gaacggcctc ccggatctcg ccgatgagca
     6721 gcttggcggg ccggaacgcg gagtcgtcgc gctcgcccca gagcaggcgg atccgacatc
     6781 cgcgtcgtgc cacccccgcg agatcgccca gcagcgtgcc gccgccgctg acctttcgac
     6841 tgtcgaagcg ggttctccgg accaggtcca cctggatgtc caccgtctcc tcggagacgc
     6901 tcgcgggatc gctcagcatg ttcacgagca ggttgtgccg gcagacctct cgaaacagcc
     6961 ggtcatcccc gccagcctcc ttgtagctgc ggatcggccg ctcgccgaac ctccgcatcg
     7021 ggaagccgcc gggcgagatc aggccgagat ggctcacgcg cggcccgaga cgccgggcca
     7081 tgttggccgc gatcgcggca ccgaacgaga agccggccag ccgcagcggc gccgcgcccg
     7141 gcagcgcctc gaggagcagc tcgtggacga gatcgaggta ctgcgggccc gtggtctcgc
     7201 gcggcaccgg ggccgaatcg ccgtaggagg gatgatcgag cgcgtgcacc gtgaaacgct
     7261 cgccgagcgc ctcgacgttg cggatccagt gcttccacga gcccatgccg ccgtggaaga
     7321 gcacgaggtc cggcccgcgg ccgagccggc ggaccgccat ccggggcgcg gtcacgcgag
     7381 cttgggtcgg atgcgctcgg cgaagagcgc catctgctcg tcgagctcgg acccgtcggc
     7441 ggccaggaac tcgagcacga ggtgctcgac gcccaccgcg cgcagctcga tgatgcgttc
     7501 gatcagggcc gacggctcgc ccaccagcac gctcccgccg cgcacggacg tcccggcggg
     7561 ccggccgggc cgcagcacgc agagatcatt gcgcagcgcg atcgggggcg gcaccgcgcg
     7621 cccgcgcgcc tggcagagcc gcgccagctc ggcccgcccg tcgcgcagct cctcgacgga
     7681 gcggttgatc ggatgccacg cggcgccgat ctcggccgcg cgacgcagcg aggcgcgccc
     7741 gtggccaccc acccagatcg gcgggtggag tttctgcacc ggcttgggct cgaacttgag
     7801 cccgctgaac cgtgtgtact tgccggcgaa ggacggcgcg gggctggtcc agagctcccg
     7861 catcgcggcc aggtactcgt cggtcatcgc gccgcgctcc gcgaagggca cgccgagcgc
     7921 cgcactctcc tgcgccagcc accccgcgcc gacgcccagc accacgcgcc cgccggagag
     7981 cgcgtcgagc gaggagacca tcttggcggt caccaccggg ttgcggtagg gcacgatgag
     8041 cacggtggtg ccgaggcgca cacggctcgt cgcgcccgcc gcgaccgcga gggtcaccag
     8101 cgggtcgtag atgccggccc cgatccggtc cacgttggcg tcgggcacca ccacgtggtc
     8161 gctgacccag accgagtcgt aaccgagctg ctccgcccgg accgcggtgc ggcggatgat
     8221 gtccggcgtg gccaggtgcc ggcagttggg gagccagacc ccgacctgca tcagcggttc
     8281 aggtggcgca gcggcagccc cgggcgcggc cactcgaagg agacgagcgt gccgcccatg
     8341 gagagcgtcg cgtacgcggt gcggaggtcc tggccgccga agcagacgtt ggtcaccatc
     8401 atgtcgggca gcatgtactg gtccacccgg ctgccgtcgg gccagatgtc gctcaccgcg
     8461 cccgtgatca gcgtggccac gcagacgtgg cctaggccgt ccaccgcgag cgagtcgaac
     8521 atctggtagc cgccgagccc ggcgatcacc gtgcccttct ccccgcggta cggcccgttg
     8581 gccgactcga tctggccggg tccggagagc ttgaaggccc agcagcgcgc ggtcggggtc
     8641 tcgaccacgt agagtgtctt ctcgtccggc gagagcccga tgccgttggg gcgctcgagc
     8701 gggaagatgg cttccttgat cgaggagccg tcggccttgg cgtagtagac gacgccccgg
     8761 tcgctgtcgc gctcgcgcgt cttgccgaga tccgtgaacc agaacccgcc cgcgccgtcg
     8821 aacaccagat cgttcggccc gcgcaggcgc tggccgtcgc accggtcgta gagcgtgtcg
     8881 aacctgcccg tctcgggatc gacgatctgg atgctgcccg tcgtgtagtc gtccgcctgg
     8941 gccaccggga agagcttgcc cgggcggtcg acgaacttga ggccgccgtt gttggtcacg
     9001 tagatcttgc cgcccgggcc caccgcggcg ccgttggggc cgccgccgag cgtggcgatc
     9061 acgtggatct tcccgtcggg ggtcacgcgc gagagcgtgc ggcgctcgat ctccaccagg
     9121 atgacggagc cgtccggcat cgccaccggc ccctccggga accgcagtcc ggaggtgatc
     9181 tgcttgaacg tgggcttgtg ggtggccatc aggattgcgc ctgccgcatg agcgctgcgc
     9241 agcgatcgag cacggggagc acctcgtcgg ccttcgcggg cgggaggctg aagatgagcc
     9301 gcgccacgcc cgcgtcgcgg tagcgcttga tgaggtctcc gtcctcggcc accccgaaga
     9361 cggtgatggg cagcgtggcc ggatcgcgcc cggcctccac caccatcttg cggaactcgg
     9421 gcagcttggc gagcacgtcg ccgtaggcgg gccggcgcgc gtgcgggacc cagccgtcgc
     9481 cgtaggcgat ggcccggcgg gcaccgtagg ggaaggcgcc gcccaccagc accggcgggt
     9541 gcggcttctg taccggcttg ggccaggtca tcatcggcgg gaacttcacg aactccccgg
     9601 tgtactcggg cttcgacttc gtccagatgg cccgcatggc ctcgacgcgc tcccgcatca
     9661 cctcgaagcg cgacttgaac tcggtgccgt ggtcggccat ctcctccgcg ttccagcccg
     9721 cgccgatccc gaacaggaag cgcccgcccg agacctggtc caggctcgcc accgccttgg
     9781 cggtctggat cggatcgcgc tgcacgacca ggcacacgcc ggtggccacc tgcagcgtcg
     9841 tggtcaccgc ggccgccgcc gcgagcgtga cgaacggatc catcacgtcg tagtacttct
     9901 tcggcagatc gccgccctgc gggaacgcgg aggcccgcga gagcgggata tgcgagtgct
     9961 ccggcgccca gagcgagccg aacccgcgct cctccagcgc ccggccgagc gccgccgggc
    10021 cgatcgagta gtcggtgaag aagatcgccg cgcctgcgtc catgtggcct cccttggagc
    10081 tacccgcgcg aatctaccgc cgtcggcagg ggcggtcaac cgtgctagga tgccgggcga
    10141 tacgcgatct ccccaggagg accccatggc aacgaagctc atggctcggc tggcgctggt
    10201 ggcgatgacc ggcgcgctgc tcttccttcc caccggcgcc accgcccagg aagtgatccg
    10261 catcggctgc cccaccaaga cctacttccc gaccatcctc gccaccgtcg ccaaggagaa
    10321 ggggctcttc gagaaggagg gcctccggcc ggagatcacg atctaccgcg gcggcgggga
    10381 gaccttcgag gcgctcgccg cgaactcggc cgacctgggc ccggtggcgg tgccgctcgt
    10441 ggccacgtcg cgcaaccgcg gcgtgctcac caagatcgtc ggcggcaacg gcgacgagtg
    10501 gtcgggctgg atcctgggcg tcaggaccgc ctcgccgatc aagtcggcga aggagctcga
    10561 cggcaagaag gtcggcatca cctcggccgg ctcgggcacc gacacgctcg ccctctgggg
    10621 ccagagcgag cacaaggtca ccttccagcg ggtgccggtg ggtgggggcg ggctcgtgcc
    10681 caacctgctc aacgacaatc tcgccgccgc ggtgatctac tcgccgctca gctaccaggt
    10741 cgtctcggag ggcaaggtgc gcgtgctgat ggacttcgcc accgcgatgc ctccgaacct
    10801 gatcggcggc tggaccgcga ccgagaagaa cctgaccgag cggcggccgt tcatccagaa
    10861 gggactcaac gcgctctacg gcgcgctcga gtacatgcgc aacaatcccg actacgcgat
    10921 caagaccatc gccaccaaca acgagctgcc gatcgagatc gccaagatgg agtacgagaa
    10981 gaccttcctc cggctctccc gcgacgggaa gatgacgctc gcctcggtgc agaaggccat
    11041 ggacctggcc gccgcgagcg gagtgaagga catggcgccc gccgccgacg tcttcatgaa
    11101 catgccgatc agcccgacca agccgtaggg gcccggcgat gcggcggcac gagcacgtca
    11161 tcgtcgccgc cgccgtcctc ggcctcttcg gcctctggga ggcgctggtc cgcctgggac
    11221 ggctcgaccc gagcctcgcg cccgcgccga gcacgatcac gggcgccctg gtccggctgc
    11281 tcgagcggcc cgaggtgctg gcgagcctcg cggtcaccgc gtgggaagcg gtcgccgcct
    11341 tcctgatcgc ggtgccggtg ggtctcctca tcggcttcac cctggccgag atccccgggc
    11401 tgggcggcct gctccggccg ctcgtcaatt tcctgttcgg ggtgcccaag tcgatcttcc
    11461 tgcccgtgtt catcctgatg ttcggggtga gcatcccgca gaagatcgcg ttcggggtct
    11521 tcaccaccgt cttcgtgctg atcatgggcg gtatcgcggc ggtgcagtcg gtcccgcacg
    11581 agctggtcac ggtcagccgc gtgtacgggg cgagccggct ccagatcatc cgggagatct
    11641 acctgcccgc gatggcgccg atcctgctcg agtcggcgcg cctcggcatg gtcttcaata
    11701 tcaccgcggt gctcctgtgc gagatctacg gcgcgcgcga cggcatcggc taccgcatcg
    11761 cggcctgggg cgagaacctc cagatgcccc agctctacgc cgcgctcgtg atcgtggcca
    11821 cggccgcggt cgcggtcaac gaggcgctgc gcctggtcga aacccggctc ggcgcgtggc
    11881 ggcagcggtc gtgacccgtc ccctggtcgc gatccgcggg gtgtcgcagc gctttggcgt
    11941 ggacggggcc agcgtggccg cgctggaggc gatcgatctc gacatcccgg acggccagtt
    12001 cgtctcggtg gtcggcccca gcggctgcgg caagtccacg ctgctctccc tcgtcgcggg
    12061 cctgagcacg ccctcgagcg gcaccgtcct ctgcgacggc gagccgatca ccgcgccgat
    12121 gccccggaag gtgggcgtga tcttccagga ggccaacctc ctgccctggc tctccgccat
    12181 cgacaacgtg gcgttcccgc tgaagctgcg ccgggtgccg aaggcggagc gcctggaggc
    12241 cgcggcccgc atgctcgagc tgaccggcct cgcgggcttc gagacccggt tgccgcacca
    12301 gctctcgggc ggcatgaagc agcgcgtggc gatcgcgcgc gggctggtgc agaacccggc
    12361 ggtgctgctg atggacgagc cgttcgcctc gctcgacgag cagacgcgca tggtgctggg
    12421 cgacgagctg ctgcgcatct ggtcggagac gcggaagacg gtgctgttcg tcacccacag
    12481 cctgaacgag gcggtgtacc tcgccgaccg cgtggtcgtg ctcagcgccc ggcccggacg
    12541 cgtggtggac gacgtggacg tcgatctccc gcgcccgcgg agcttcgcga tgacctcgag
    12601 cgagcggttc ggcgtcctca aggaccggat ctggcagcac atcaagcacg cggagcgccg
    12661 atgacccgcc tgcgctgggg ccaggccgcg ctgtacctgg ccattctcgc ggcctgggag
    12721 ctgctgccgc gcgcgggcgt gatcccgcgc ctcttcgtgc cgccgctgag cgaggcgctc
    12781 ggcgccctcg tcgccgaccg gcgcgagtac ctcggcagcc tgcccaccac cttcggcgag
    12841 atcctcgtct cctacgtcat cgtctgcggc ggcggcatcc tcctgggcca gctcatcggc
    12901 gcctcggcgg ccgcacgccg catcttgctg ccggtgctgc ggagcgccta cgcggtgccg
    12961 ctggtcgtgc tctaccctgt gatgatggtg tggttcggcc tgggctcgga gtcgaagatc
    13021 gccttcgcct cgatctacgc cttcatcccc accctgctga ccgcggtggc cggcgtggcc
    13081 gcgctcagcc ccgcgctcgc cgagaccgcc cgcgccttcg gggccacccg cgctcagcag
    13141 atcctctacg tctccctgcc cgcgagcctg ccgtccatcg tggccgcgct gcggctcggg
    13201 ggcgcgctcg tgatcgtcgg cgtcatcgtg gcccagatgc tcggcgcggc cgacggcctc
    13261 ggctacctga tcacgcggca ccggacgctg ctcaacagcc ccggcgtcta cgcgggcatc
    13321 gtgctcgtgc tcgggatcac cgggatgcac gagattcttc tccgccgcct ggagcacaag
    13381 gtggcggcct atcggcagtc ctgacccgaa ggaggtccag gccatggttc agcaggcgct
    13441 caccaccacg gtggtcggca gctatcccca gcccgactgg ctcgtcgatc gggccctgct
    13501 gtccaagggc gtcccgcggg tgcgcctgcg gagcatctgg cgggtaccgg acgatctcct
    13561 ggagcaggcc caggacgacg ccaccgtggt ggccatccgc gacatggagc gcgccggcgt
    13621 cgacatcatc agcgacggcg agatccggcg cgagagctac tcgaaccggt tcgccaccgc
    13681 gctggacgga gtggacgtga accagcccgc ggtgatcacc gatcgctacg gcaaccagac
    13741 ccaggtgcct cgcgtggtcg ggccgatccg gcgggtccgg ccgatcgagc tgcgcgacat
    13801 gcagttcctc cggcgcaaca ccgaccggcc cgccaagatc accctgcccg gccccttcac
    13861 gatggcccag caggtccgga acgagcacta ccgagacctg gaagagctgg cgatggacta
    13921 cgcggaggcg gtcaacgccg aggcccacga cctgcaggcc gcgggcgccg acgtcatcca
    13981 gctcgacgag ccctggctgc gcaacgaccc cgaggcggcc aagcgctacg cggtgcccgc
    14041 gatcaaccgg gcgctcgccg gcctcaccgt gccgaccgcg ctgcacctct gcttcggcta
    14101 cgcggcggtg gtgaagcagc agaagcccac cggctactcg ttcctgcccc agctcgcggg
    14161 caccatcgcc cagcagatct cgatcgaggc cgcgcagccc aggctcgacc tgggcgtgct
    14221 gagcgatctg gccggcaaga cgatcatcct cggcgtgatc gacctcgcgg accccgcggt
    14281 ggacacgatc gagcaggtga cgtcgcggat ccgggccggg ctcgcccggg tgcccgcgga
    14341 gcggctgatc ctggcccccg actgcggcat gaagtacctg ccgcggcaca ccgcgttcaa
    14401 caagctctgc atgctgaccg ccagcgcgcg cgccgtccgc gcgcagctcg cctaggagga
    14461 cccgatggcc gtgctactgc tcgacgatcc cctgcccggc gtccgccgcc tgacgctcaa
    14521 ccgcccggag aagcgcaacg cgctcagcaa cgagctgcgc ggcgcgatcc tcgacgccct
    14581 gaaggcggcc gacgcggatc ccgcggtccg tgtctcggtc ctccgcggcg ccggccccgc
    14641 gttctcctcc ggctacgacc tctccgccga caactcggtc aaccagccct actactcccc
    14701 cggcggcccc gggcaatggg cgcggcacgt ggtggacggg tggttcacga tctgggacct
    14761 ggccaagccg gtgatcgccc aggtgcacgg ctactgcctc gccgggggca gcgagctggc
    14821 cgcgtgctgc gacctggtct acgtggcgga ggacgcgcgc atcggctacc cgcccacgcg
    14881 cctgatgagc ccgcccgaca accagttcca cccctggctg atgggcatgc gcaccgccat
    14941 ggagatgttc ctgaccggcg acgcgatcac cggcacggaa gcggccgcgc gaggattcgc
    15001 caaccgcgcg gtcgcggcgg cggacctcga gcggaccgtc ctcggcgtcg ccgagcgcat
    15061 cgcgctggtg ccgaccgaat tgacgcaaat aaacaagaga tcagtccacc gggcgatgga
    15121 gatcatgggg ctgcgcgccg ccatccgggc cggcaccgag ctgcaggcgc tcgcgttcac
    15181 cacccaggcc agcctccagt accgcgccca gttcaagcgc gacggcgaga gcgtgcgcga
    15241 cctgctgagc cagcgcgacg ccgcgttcgg cgactaccgg gagcgcgagc gctgagggcc
    15301 tagcccctca ccctgccctc tccccggagg ggagagggtg tagtccgaac cctcgccccc
    15361 ggagggggag agggcagggt gagggggcgc gctaggccac gcccacgaac ccgatatacg
    15421 ccccgcaggc gtgctcgggc ccgacgagca tcgcctgctc accggtattg cgcaccccgc
    15481 gcgcgggcgt cggcaccccg cgctcggcca ggacgcgcgc caccgcgtcg aggctgcgca
    15541 cgcggtggag cacctggaag gggccgggcc cgcgccgggc cagcgcctcc gcggcggggc
    15601 cgggctcggc gggctgggcc agcgtgagcc cggtgggacc gagatcgaac accgccatgt
    15661 ccgccctgat cacggcgccg cgctggatcg cgggcaccgg cagcccgagc acctgcgcgt
    15721 aggtccgggc ttccgcggcc acgtcggtca ccgcgatata gacgcgcacc acgcgcatca
    15781 cgccgttcgg atggctcccc agctggaccg cccgggcctt ccgctcggcg agcggcgtca
    15841 ggtgctggat gaggaagatg ggcagggcgc tggacgggcc gaggaaggcg gcgcgccagc
    15901 gcagctcctg gccctccggc gtgcgccgcc cgccgtcgcg gatctcgccc acgtccacgc
    15961 cgcgccggcg catcgcggcc acgtcggcgg ccagatcatc gctctgcagc gccacgatcc
    16021 ggaagccgcc gccctggtcc aggtactcgg cgaggctcgc atccgggctg ccgggtacga
    16081 ccgcgcgccg atcgcggatg ctcaggagct cgagatagtc ctcgtcgtgg aaggcgatcg
    16141 cgttgtgggt cggccgaccg gtgtgcgagc cgccggggta gacggtgaac ccgagacgcg
    16201 tgtagacctc gatccccttg gcgaggtccg gcacgcagat catcacgtgg tcgatgcggg
    16261 tcagcatgcg gccgcgctcc tagcagccct tgcagggcgg gtagtcgcgg ttgtacaccg
    16321 gctgcttcag gaattcctcc ggcttgtagg tccagaactg cgagaccgcc gggaaggtgt
    16381 ggacgacggt gttctgcagc tcgcccccct tccgctccac cttgcggacg tagatgttct
    16441 ggatcgggtt gccccactgg tcgagcttca ccggcccgcg cggcgcgtcc gggatctcga
    16501 ccttgcggaa cgcggccatg aacttctcgc gctcctccac gttgccgccg accagcttcg
    16561 cggcctcgct gatccagcgc gcgctcgtat agcacgtctc tgcgaagtac gagggcacct
    16621 tgccgtactt cttgcggtag tccgccacga acttccggtt cgcgggcgta tcgagcgcgg
    16681 cgctgaacat gaggggcgtg acgtagccga gcgtctcgtc gccgaagctc ggcaggatgg
    16741 actcgtccac gatcaccgcg ccgccgatca ccgggatctt cccgtacagt cccgcgtccc
    16801 ggtactgggc cgggaagcgg agcgaggacg ggacggccag gatgacgaag accgcgtcgg
    16861 cctcgcgctt gatctggctg aggtaggggg ccagatcgtt ggtgccgagc ggtgcccaca
    16921 gcttctggat gatctgccca cccgcttcct cgaagctccg ctggaaacca cccaccacct
    16981 cgtagccgaa ggcgtagtcg cttccgaaca ccgcgacctt cttgtagccc agggtcttcg
    17041 ccgcgtactc cccgaacggg tgcgacggct ggctcccggt ccagccgagg cgcaccaccc
    17101 acttcgacgg cttccgctgg gtgagatcgt cggccgcgat caccgggaag atggtgggca
    17161 tcttgaactc atcggccttc ggggccaggg cgtacccgat gtgcgcgaac acctcgccca
    17221 ccaccatgtc cacctgatcg ctctcgacga acttgcggaa cttggtgatc gcggtgcccg
    17281 ggttgcccgc ggtgtcctcg acgatcagct cgaccttccg gcccgcgatc tggtgccccg
    17341 cctcctccag gtacatctcg aacccgttga cggtgtcgcg cccgacctgg gccgcaccgc
    17401 ccgtgagcgg ggccaggaac ccgaccttga tcggcttctt cgcctgcgcg gcggcgggcg
    17461 gcgccggcgc gaccgcgccg gtcaagaggg cgagacccag gagccagatc gagagcgcgt
    17521 gtcgtgggcg catggctatc tcctcgctga gatgctgggg tcgtcgccgg ccagccgcgc
    17581 gaacaggcgc ttcatgccgt tgacctgcgc gagataccgg gcgacgtcgt ggtcgccgca
    17641 ctgctggccc ggcgtgaggc ggaagccgcg gcgcgcgtgg gcgtcccccg ccccctcacc
    17701 gcagagatgg atgagagccg cgagatcctg cttctgtcgg ggagtcgcgg tcacgatgcg
    17761 ccggcgctcc agcgtgcggg ccacgccgcg atcgagcatg gccgaggtca gctcgaccgc
    17821 gtggcccggc agcacgcgcg tgtagaggct ccggaaccag cactggtcct cggccaccac
    17881 gtgatcgcgc acgcagaagc gcttcgcctc ccggaaggtg ggatcggtga tctggtacat
    17941 gccgaccgcg ctcgaggcgg gacgatacac ctcgaagggg tgccacgcca cctgccaccg
    18001 ccagtaggtg cgggccaccg gatcacccga gccctcgacc tgggcgagcg cggcgagcag
    18061 ctcgggcgtg atggtggcgg tcgcgtggcg gcggaagagc ggcgcgtagc ttcgccacgt
    18121 ctcggccggg gccttgctca gcgtgccgct gaccgggaag aacagctcgc tgggcttgcg
    18181 gatcacctgg tacgtccagt tcgccgccga ccagacgatc acgagcgcca tggcgaaccc
    18241 gacgatgcgt accgcgagcg gcgcggcgag caacgccagg cctgcgcgct tgagcccacg
    18301 gcccagcgac cgcagccccg ggcgcaggcg cgtgaaccgg gcccgccgcg cccggggctt
    18361 ccggggcgcg cccctgccgg tccgcgcacg agcgatcggc gccatgcctc gactctactg
    18421 cctcccggga ttccgggtaa cccctaccac ccgtcatcgt gggcctcccg cgatgaagtc
    18481 ccagacctgt tcgggagctt cccagtgggc attgtggccg acgccctcca agacccgcgc
    18541 ggcgggatcg atgcgccgca tctgctcgag ggtgaccatc gggtcccgat cgcccgcggc
    18601 caggcgcagg ggtgtctccg cgaggcggag caggccttcg accgacgggc cgaccgcacc
    18661 gtaggcgcgc ggatccatgg ccacctggaa gcggccatcc gtgccggtca cgccggcggc
    18721 cgcggtggcc gaggaggccg gcgccagacc cgcgagcccc gagatccgca ggtagcgcac
    18781 ggtcgcctcg tcgcgggtgg tgaaaacggg cgcgggacgg ccggccatct cgcgggcctt
    18841 cgccatctcg tccagactcc actcgatctt gacgccgaac gccgcgacgt cgcgcacgcg
    18901 cggaccgaac cagccgctgg ccaccagggc ggccaccgcc ccgccgaagg aatgcccgac
    18961 cagcgtgacc gggcccgccg cctccgtctc gatgagcccg gccacgtcgg cggcgtgcat
    19021 gccgaacccg tacgggcctt cccgcaccga acggccgtgg ccgcgcaggt cgggtgccag
    19081 ccaccggccg cgccaggcgc cctcgatcag cggcagcagg cgctcccaga cgccggcgtt
    19141 ggcgcccatg ccgtgcag
//
LOCUS       DASUFA010000415         6316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1488922, whole genome shotgun sequence.
ACCESSION   DASUFA010000415 DASUFA010000000
VERSION     DASUFA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488922"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFX87_15055"
     CDS             complement(<1..956)
                     /locus_tag="VFX87_15055"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX4996300.1"
                     /translation="MDPIHTLVTHLGRARYEDLSAEVVETVKMTILDTMGAALAGSAS
                     EAGRSIARIAKGYGGGTGSTVVVHGGKVAPPLAVLANGIMARCRELDGTHEVGGGHVG
                     VCIVPAAFALAEHSASPVSGKDVILAVALGVDMLCRLRMGAGKAKAIGWMAETMAPLS
                     VAAMGAKLLALSEREMLDAVGVAYALCSGNVQPTVEGAWSLWVPAGTAAAGGILAIDL
                     ARAGFLGPRNPLLGEFGLYQLYFRGEYDEAQLLGQLGDRNEILNSSLKPYPTCKYTHH
                     AICTTLKLVEEHDLEPEQIKGVAVATSTMGATQCGFDASGTP"
     gene            complement(985..2037)
                     /locus_tag="VFX87_15060"
     CDS             complement(985..2037)
                     /locus_tag="VFX87_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996301.1"
                     /translation="MKIDFGIGLGSSVGLLGGADSQKFMDLVRMADGYGAAAIGTYDS
                     AFLGGDAYVRATLMALAAPRAQIGLRPSNPLTREPQIMASFLASIDQLTGGRAFLDIA
                     SGDSAVFNIGYPAASRARIEDYVTCVRGLLARGEATYQGRAQRVRWANEAVRPRVPIS
                     ICAEGPKMLHLGGRIGDGVVAGTGLLPEVVRDTIARVHAGAREAGRAPGDVDVWFTAR
                     SSLHEDREKAIDNVKASVSSILNHAMRFGLDDKHVPDDLRARIQDYVDGYELYDHVLH
                     AGRNPKRMADLGLTDYAIDRWALAGAPRDWIARIEQLAERGARKLWVGVSGGDLDGQR
                     HYLKLMGEEIMPRFVG"
     gene            complement(2034..2888)
                     /locus_tag="VFX87_15065"
     CDS             complement(2034..2888)
                     /locus_tag="VFX87_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4996302.1"
                     /translation="MKTLRGRVAVVTGAASGIGLALAERFASEGMKIVMADIEASALT
                     TAADGLRRRGATLLATRVDVSRPEDVERLARETYQAFGAAHVLCNNAGVAVIGAVHEH
                     TLADWQWVINVNLWGVIHGVRTFLPRMLGGGDEGHIVNTASMAGLTTAPFMSVYDVTK
                     HGVVALSESMYKELQATGAPIGVSVLCPGLIDTKIMRSARNRPDELAEEGKAGPMAQA
                     FGQALSDRLAGGYPPSEVAEQVFQGIREDRFYIVPAQPEVKGGIVVRAQDLLELRNPT
                     LRDLRPPR"
     gene            complement(2885..3655)
                     /locus_tag="VFX87_15070"
     CDS             complement(2885..3655)
                     /locus_tag="VFX87_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4996303.1"
                     /translation="MDPRLDVMVEAARAGGATALEHFRRQGFAVSLKADRSPVTEADR
                     AAEGAILDVLRAGCPDHGALGEELGESGPRDRRFIVDPIDGTRNFVRGIPTWAVLIGL
                     EEDGQVTAGVVYQPVTGTLHTAWRGQGAYRDGARIRVSSVDALDRALVVHSSVNFLRR
                     STYWDGFLRLADRTQVQRGFGDFSAYLWVAEGLGEIALSTTVKAWDVAALKVVVEEAG
                     GQVTDLDGRSGIYGSTVFASNGLLHDAALAAMRKEERA"
     gene            3711..4289
                     /locus_tag="VFX87_15075"
     CDS             3711..4289
                     /locus_tag="VFX87_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4996304.1"
                     /translation="MAPETPTDGPRFTLLTEATMTPRQRESYQGIVSGPRKGARGPFN
                     ALLRSPEVADRVQKVGEYIRFQSTIPAALNEMAILITGRFWSAQYEFYAHSRLARTAG
                     LPDPIIDAIAEGRRPSSMSDDQRIVYDFCTELFRDKAVADATFKAAVDRFGEQGVIDL
                     IAASGYYSIVSMVLNVDRYPLPAGERPPLRPL"
     gene            4411..5772
                     /locus_tag="VFX87_15080"
     CDS             4411..5772
                     /locus_tag="VFX87_15080"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX4996305.1"
                     /translation="MADAPSRFVRQPTPAERSELVRLRDTGPAGPRARAAVVLLSATR
                     VAIPEIVRRLGLSRPTVTHWLRRFERSGIAGLRDRPRVGRPARISSDVLRHISMVVSA
                     RPRDLGIPGTRWSLAGLRQYLVQAGVVPALSEESLRVALKRAGLAVTSSRAGQIQKGV
                     TTMIPGAFEYHAPSSIGEATALLARLGDDAKILSGGQSLIPLMKLRLATPRHVIDING
                     LPGLAYVREDAGALRIGGLTREVDLEESDLVRSRYPLLFDTCKVIADPIVRNLATIGG
                     NLAHGDPANDHPAAMLALGAEIVAVGGRGERKIPITSFFTGPLSTALKADEIVVEIRV
                     PAPKPGSGGAYVKMERKVGDFATAAVAVQLTLGAGGACEEVGIGLTNVGSVPIKAERA
                     ELALKGKRPDEATIKQAAQLAADAAQPEADLRGSVEYKKDVVRVLTGRALRRAIERAQ
                     GGS"
     gene            5777..6247
                     /locus_tag="VFX87_15085"
     CDS             5777..6247
                     /locus_tag="VFX87_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012715913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4996306.1"
                     /translation="MLVKVAINGEKHEAEVEPRLLLVHMIRENLRLTGTHIGCDTSHC
                     GACTVLLDGRPVKSCTVFAVQAHGREITTVEGLEQDGKLHPLQEAFMTEHGLQCGYCT
                     PGMLMTGVAFLKKHPRPTEGEIRHAISGNLCRCTGYVNIVKSIQRAAGTLQGGA"
BASE COUNT          934 a         2311 c         2160 g          911 t
ORIGIN      
        1 ttgggcgtgc cgctcgcgtc gaacccgcac tgggtggccc ccatcgtcga ggtcgcgacg
       61 gccaccccct tgatctgctc cggctcgagg tcgtgctcct cgacgagctt cagggtcgtg
      121 cagatcgcat gatgcgtgta cttgcaggtg ggatagggct tcaggcttga attgaggatc
      181 tcgtttcggt ctcccaactg cccgagcagc tgtgcctcgt cgtactcgcc gcggaaatag
      241 agctgataga ggccaaactc cccgaggagc ggatttcgcg gacccagaaa tcccgccctg
      301 gccagatcga tggccaggat tccgcccgcc gccgcggtgc cggcggggac ccacaagctc
      361 caggctccct caacggtcgg ctgcacgttg ccgctacaca gcgcataggc cacgccgacc
      421 gcatcgagca tctctcgctc ggagagggcg agcagcttgg cgcccatcgc cgccacactg
      481 agcggcgcca tggtctcggc catccagccg atcgccttgg ccttgccggc gcccatccgc
      541 agccgacaga gcatgtcgac tccgagcgcc acggccagta tcacgtcctt cccgcttacc
      601 ggcgaggccg agtgttccgc gagcgcgaag gcggccggaa cgatgcacac gccgacgtgt
      661 ccgccgccga cctcgtgggt cccgtccagc tcgcggcatc tggccatgat gccgttcgca
      721 agaaccgcca gcggcggagc gacctttccc ccgtggacga ccaccgtgct gccggtaccg
      781 cctccgtacc ccttcgcgat ccgagcgatg ctccggcccg cttcactggc gctgccggcg
      841 agagcggcgc ccatggtatc gaggatggtc atcttgaccg tctcgacgac ctcggcggac
      901 aggtcttcgt agcgtgcccg cccgagatgt gtgacgagcg tgtggatcgg gtccataagg
      961 tggcctccag tccggccgcg ccggctatcc gacgaacctc ggcatgatct cctcgcccat
     1021 cagcttcagg tagtggcgct gcccgtcgag gtcgccgccg ctcacgccca cccagagctt
     1081 ccgggcgccg cgctcggcga gctgctcgat gcgcgcgatc cagtcgcgcg gcgcgccggc
     1141 cagcgcccag cggtcgatcg cgtagtccgt caggccgaga tcggccatgc gcttgggatt
     1201 gcggcccgcg tgcagcacgt ggtcgtacag ctcgtagccg tcgacgtagt cctgaatcct
     1261 ggcccggaga tcgtcgggca cgtgcttgtc gtcgaggccg aagcgcatcg cgtggttcag
     1321 gatcgacgac accgacgcct tgacgttgtc gatcgccttc tcccggtcct cgtgcagcga
     1381 cgagcgcgcg gtgaaccaga cgtccacgtc gcccggggcg cggccggcct cgcgggcgcc
     1441 ggcgtgtacg cgcgcgatcg tgtcgcggac cacctccggc aggaggccgg tgccggcgac
     1501 gacgccgtcg ccgatgcgcc cgcccagatg gagcatcttc ggcccctcgg cgcagatgga
     1561 gatcgggacg cgcgggcgca ccgcctcgtt cgcccaccgc acgcgctggg ctcggccctg
     1621 gtacgtcgcc tcgccgcggg ccagcaggcc gcgcacgcac gtcacgtagt cctcgatgcg
     1681 cgcgcgggag gcggccgggt agccgatgtt gaagacggcg ctgtcgccgc tggcgatgtc
     1741 caggaaggcc cggccgccgg tgagctgatc gatcgaggcc aagaacgaag ccatgatctg
     1801 cggctctcgg gtgagcgggt tgctcggccg cagcccgatc tgcgcgcgcg gcgcggccag
     1861 cgccatcagc gtcgcccgca catacgcatc gcccccgagg aacgcggagt cgtaggtccc
     1921 gatggccgcg gcgccgtagc cgtcggccat cctcacgagg tccatgaact tctgcgagtc
     1981 ggcgccgccg aggagcccga cgctcgagcc gaggccgatc ccgaagtcga tcttcatcgt
     2041 ggcggcctca gatctcgaag ggtcgggttg cggagctcga gcaggtcctg cgcgcgaacc
     2101 acgatcccac ccttcacctc gggctgcgcg ggcacgatgt agaagcggtc ctcgcggatg
     2161 ccctggaaca cctgctccgc cacctcggag ggcgggtagc cacccgcgag gcgatccgag
     2221 agagcctgcc cgaacgcctg ggccatcggc ccggccttgc cttcctcggc gagctcgtcc
     2281 ggccggttgc gggcggatcg catgatcttg gtgtcgatga gcccggggca gagcacggag
     2341 accccgatcg gcgcgccggt cgcctggagc tccttgtaca tggactccga gagcgcgacg
     2401 accccgtgct tggtgacgtc gtagaccgac atgaacggcg cggtggtgag cccggccatg
     2461 gacgcggtgt tgacgatgtg cccctcgtcg ccgccgccca gcatgcgcgg caggaacgtg
     2521 cggacgccgt ggatcacgcc ccagaggttc acgttgatca cccactgcca gtcggccagc
     2581 gtgtgctcgt gcaccgcgcc gatcaccgcg acccccgcgt tgttgcagag cacgtgggcc
     2641 gcgccgaagg cctggtaggt ctcgcgggcc agccgctcca cgtcctcggg gcgcgagacg
     2701 tccacccggg tggccagcag cgttgcgccc ctccgcctca gcccgtcggc cgcggtggtg
     2761 agggccgacg cctcgatgtc ggccatgacg atcttcatgc cctcgctggc gaagcgctcg
     2821 gcgagagcga gtccgatccc actcgccgcc cccgtcacga ccgccacccg accccggaga
     2881 gtcttcacgc gcgctcctcc tttcgcatcg cggccagcgc cgcgtcgtgc agcaggccgt
     2941 tgctggcgaa gacggtgctg ccatagatgc cggagcggcc atcgaggtcc gtcacctggc
     3001 cgcccgcctc ctccacgacg accttcagcg cggccacgtc ccacgccttc acggtggtcg
     3061 agagggcgat ctcccccaac ccctccgcca cccagaggta ggccgagaag tcgccgaagc
     3121 cgcgctggac ctgcgtgcga tcggccaggc ggaggaagcc gtcccagtag gtgctgcgcc
     3181 gcaggaagtt cacggaggag tgcacgacca gcgcacggtc gagcgcgtcc accgaggaca
     3241 cgcggatccg cgcgccgtcg cggtaggcgc cctgcccccg ccacgcggtg tggagcgtcc
     3301 cggtgaccgg ctggtagacc acgcccgccg tgacctggcc gtcctcctcc agcccgatca
     3361 gcaccgccca cgtggggatc ccccgcacga agttccgggt gccgtcgatg ggatcgacga
     3421 tgaagcgccg atcgcgcgga cccgactcgc cgagctcctc gccgagcgcc ccgtgatcgg
     3481 ggcagccggc gcgcagcacg tcgaggatcg cgccctcggc cgcgcggtcg gcctcggtga
     3541 ccggcgaccg gtccgccttg agcgacaccg cgaagccctg ccgccggaag tgctcgaggg
     3601 ccgtcgcgcc cccggcgcgg gcggcctcca ccatgacgtc gagtcgaggg tccacgcaga
     3661 gagcttaccg cggtatgctg tgctgccact cactcacgag gaggagaccg atggctcccg
     3721 agacgccgac cgacggcccc cgtttcacgt tgctcaccga agccaccatg accccgcgcc
     3781 agcgcgagag ctaccagggc atcgtgtcgg gcccgcgcaa gggcgcccgt gggcccttca
     3841 acgcgctgct gcgcagcccc gaggtggccg accgcgtgca gaaggtcggc gagtacatcc
     3901 gcttccagtc cacgatcccg gccgcgctca acgagatggc catcctgatc accggccgct
     3961 tctggagcgc gcagtacgag ttctacgcgc acagccgcct ggcccgcacc gccggcctcc
     4021 ccgacccgat catcgacgcg atcgcggagg gccgccgccc gtcctcgatg agcgacgacc
     4081 agcggatcgt ctacgacttc tgcaccgagc tgttccgcga caaggcggtc gccgacgcca
     4141 cgttcaaggc cgcggtcgac cggttcggtg agcagggggt gatcgacctg atcgcggcca
     4201 gcggctacta ctcgatcgtc tcgatggtcc tcaacgtgga ccgctacccc ctgcccgccg
     4261 gagagcgccc gccgctccga ccgctctagt cgagtcccgc ttgacagcgg ccgggccgtc
     4321 tcgtaccgtc ccggccgtcg gggactttgg gcctgaccgg aaagtccctt gccatgattc
     4381 tcccggcctc cctcaaggga gaggtgatgg gtggcggacg cccccagccg attcgtgcgg
     4441 cagccgaccc cggccgagcg ctcggagctg gtccggctcc gggacacggg ccccgcgggg
     4501 cctcgcgccc gcgcggccgt ggtcctgctg tccgcgaccc gggtcgcgat tccggagatc
     4561 gtccggcgcc tcgggctctc ccgcccgacc gtcacccact ggctgcgccg gttcgagcga
     4621 tcggggatcg ccggattgcg ggatcgcccg cgcgtcggcc ggcccgcccg gatctcttcc
     4681 gacgtgctcc gtcacatctc catggtcgtg agcgcccggc ctcgggatct gggcatcccg
     4741 gggacgcggt ggagcctggc cgggctccgg cagtacctcg tccaggccgg tgtcgtgccc
     4801 gcgctgtctg aggagtcgct ccgggtcgcc ctgaagcgcg ccgggctcgc ggtcacgagc
     4861 tcccgcgccg ggcagatcca gaagggagtc accacgatga ttccgggcgc ctttgagtac
     4921 cacgcaccca gctcgatcgg ggaagccacc gcgctgctcg ccaggctcgg cgacgacgcc
     4981 aagatcctct cgggagggca gagcctcatc ccgctgatga agctcaggct cgccacgccg
     5041 cggcatgtga tcgacatcaa cggccttccc gggctcgcgt acgtgcggga ggacgccggc
     5101 gcgctgcgca tcggcgggct gacccgcgag gtggatctcg aggagtcgga cctggtgcgc
     5161 tctcgttacc cgctgctctt cgacacctgc aaggtcatcg ccgatcccat cgtgcgcaac
     5221 ctggccacga tcggcggcaa cctggcgcac ggcgacccgg cgaacgacca tcccgcggcc
     5281 atgctggccc tcggcgccga gatcgtcgcg gtcggcgggc gcggcgagcg gaagatcccg
     5341 atcacgtcgt tcttcacggg gccgctcagc accgcgctca aagccgacga gatcgtggtg
     5401 gagatccgcg tgccggcgcc aaagccgggc agcggcggcg cctacgtgaa gatggagcgc
     5461 aaggtcggcg acttcgccac cgcggcggtg gcggtgcagc tcacgctggg cgccggcggc
     5521 gcctgcgagg aggtcggcat cgggctcacc aacgtggggt cggtgccgat caaggccgag
     5581 cgggccgagc tcgccctcaa gggcaagcgg ccggacgagg cgaccatcaa gcaggcggcc
     5641 cagctcgccg ccgacgcggc gcagcccgag gccgacctgc ggggatcggt cgagtacaag
     5701 aaggacgtgg tgcgggtgct gaccggccgc gcgctccgcc gagcgatcga gcgcgcccag
     5761 ggagggagct gagcccatgc tcgtcaaggt cgcgatcaac ggggagaagc acgaggcgga
     5821 ggtggagccg cggctcctcc tggtccacat gatccgggag aacttgcggc tgaccggcac
     5881 ccacatcggc tgcgacacga gccactgcgg ggcctgcacg gtgctgctcg atggccggcc
     5941 ggtcaagtcc tgcaccgtct tcgcggtcca ggcccacggg cgcgagatca ccaccgtgga
     6001 ggggctcgag caggacggca agctccatcc gctgcaggaa gccttcatga ccgagcacgg
     6061 cctgcagtgc ggctactgca cgccgggcat gctgatgacc ggcgtggcct tcctcaagaa
     6121 gcatccccgg cccaccgagg gcgagatccg ccacgccatc tcgggcaacc tctgccgctg
     6181 caccgggtac gtgaacatcg tcaagtccat ccagcgcgcg gcgggcacgc tccagggagg
     6241 cgcatgatgc caccgaagac atcgctcgaa gtcggcggga tgggccactc catcaagcgg
     6301 aaggaggacc cgcggt
//
LOCUS       DASUFA010000416         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1362601, whole genome shotgun sequence.
ACCESSION   DASUFA010000416 DASUFA010000000
VERSION     DASUFA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362601"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1174
                     /locus_tag="VFX87_15090"
     CDS             134..1174
                     /locus_tag="VFX87_15090"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX4996307.1"
                     /translation="MRQLKYREAIDEATRQAMAKDERVFVMGVGVDDPKGIFGTTRGA
                     FEQFGPSRVFDTPLSEAALTGMGVGAAMRGFHPLLVHARNDFLLLTMDQIVNNAAKWR
                     YMSGGKLKVPLTIRAIIGRGWGQAAQHSQSLQAMFAHVPGLSVIMPTTPADAKGLLMT
                     ALCSDSPVICLEHRWLYDKSGPVPEDPYYTPFGVGSVVRPGRDVTIVAVSHMVLEALQ
                     AAEALAGEGIEAEVIDPRTFRPLDSGLICRSAARTGRLVIADTGWRSYGVSAEIAARA
                     AEELWGVLKAPIKRVALPDVPTPGSSVLESAYYPGTREIVAAVKQCLEAPAGAMPSGL
                     APSNRKEFEGPF"
     gene            1176..2348
                     /locus_tag="VFX87_15095"
     CDS             1176..2348
                     /locus_tag="VFX87_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996308.1"
                     /translation="MPISANVVDYEQVTGSYVTTLNTQLRGFRGGARFLEHWVHDPDP
                     VKSVINMIEAAATDGLADVTVLLGGRTQAALDVGRLERLAGALGPVTREAHDGGLALT
                     VRFTRAAAAAPGLGAEARDRARRAREARDARLHAQALAATPRPAPNGLHPVYREAVQR
                     ILASALAHEGPVATMPGAERLSTARDGWVLEVFVDPATHRVRAARHRGARKETRALLE
                     GLCAVMEDKPLIECADHAVIRLEYRLRDRARERPVAGVVTPESVEPGFAPLGAAVREL
                     LAEYRRRTGYDETENRFDPEPSAGWKALTPEQRLARIQVALDGLVAGRGLRSGDVVCT
                     RVDRSTRATVEFRAELPARGKAALMMDLERGLKEAVEPVLHLYQEEMHDKNKIRRL"
     gene            2370..>3200
                     /locus_tag="VFX87_15100"
     CDS             2370..>3200
                     /locus_tag="VFX87_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4996309.1"
                     /translation="MSQALTDHTDLILDGTKIQWHLDRVRAWERGERIAPITIDMALT
                     RACNYACHFCYAMLQENDRQTITKEVITSFLDDCAEIGVKGISLVSDGESTISPVFVD
                     AVVHGSEVGLSMACGTNGFIFNRRKLEEVLPHLTYLRINISAGERKRYAEIMGVREEW
                     FDRVVQNIRDMVDIKKRESLPVTIGLQMVLDPRYGDQIMPLARLGKELRPDYLVIKHC
                     SDDEDGTLGVDYEAYDRLYDTLKEAEALADSEYAVKVKWSKIEAKGRRSYQRCYGPPF
                     M"
BASE COUNT          501 a         1106 c         1168 g          425 t
ORIGIN      
        1 cggcggcgcg gcgcgccgtg ctcgacgccc agatcgaggc cgaggtcgcg gacgcggtcg
       61 ccttcgccaa gtcgagccca ttccccgagc cggccgccct gttcgcggac gtggactagg
      121 gagagccatc cgcatgcgcc agctcaagta ccgcgaggcg atcgacgagg cgacgcgcca
      181 ggccatggcc aaggacgagc gggtcttcgt catgggcgtg ggcgtggacg acccgaaggg
      241 catcttcgga accacgcggg gggcgttcga gcagttcggg ccgagccgcg tgttcgacac
      301 cccgctgtcg gaggcggcgc tgaccggcat gggggtgggg gcggcgatgc gcgggttcca
      361 tccgctcctc gtccacgccc gcaacgactt cctgttgctg acgatggacc agatcgtgaa
      421 caacgcggcc aagtggcgct acatgtcagg ggggaagctc aaggtgccgc tcaccatccg
      481 cgccatcatc ggccggggct ggggccaggc cgcgcagcac tcgcagagcc tgcaggccat
      541 gttcgcgcac gtgcccggcc tgtccgtgat catgcccacg acgcccgcgg atgccaaggg
      601 cctgctcatg accgcgctct gcagcgacag cccggtcatc tgcctcgagc accggtggct
      661 ctacgacaag agcggcccgg tgccggagga tccctactac accccgttcg gggtcggctc
      721 ggtggtgcgg ccggggcgcg acgtcacgat cgtggccgtc tcgcacatgg tcctggaggc
      781 gctgcaggcc gccgaggcgc tggccggcga gggtatcgag gccgaggtga tcgaccctcg
      841 caccttccgg ccgctggact cggggctgat ctgccgctcc gcggcccgga ccgggcgcct
      901 cgtcatcgcg gacaccggct ggcgcagcta cggggtcagc gcggagatcg cggcgcgcgc
      961 ggccgaggag ctctggggcg tgctgaaggc gcccatcaag cgggtggcgc tgcccgacgt
     1021 gcccaccccg ggcagctcgg tgctggagtc ggcgtactac ccgggcacgc gtgagatcgt
     1081 ggcggcggtg aagcagtgcc tggaagcgcc ggcgggcgcg atgccgtccg gactcgcgcc
     1141 gagcaatcga aaagagttcg aggggccgtt ctgagatgcc gatctccgcg aacgtggtcg
     1201 actacgagca ggtcaccgga agctacgtga cgacgctcaa cacccagctc cggggcttcc
     1261 ggggcggggc gcgcttcctc gagcactggg ttcacgatcc ggatccggtc aagagcgtca
     1321 tcaacatgat cgaggcggcc gccaccgacg ggctggccga cgtcaccgtc ctcctcgggg
     1381 gccgcaccca ggcggcgctc gacgtcggac ggctggagcg tctggccggc gctctcgggc
     1441 cggtgaccag ggaggcgcac gacggcgggc tcgccctcac cgtccggttc acccgcgcgg
     1501 ccgccgccgc gcccggactc ggggcggaag cgcgggaccg cgcgcggcgg gcccgcgagg
     1561 cgcgtgacgc gcgcctgcac gcgcaggcgc tggccgcgac cccgcggccg gccccgaacg
     1621 gcctccatcc ggtctaccgc gaggcggtgc agcggatcct cgcgagcgcc ctggcgcacg
     1681 agggcccggt ggccaccatg ccgggcgcgg agcggctctc gaccgcgcgc gacgggtggg
     1741 tcctcgaggt cttcgtggat cctgcgacgc accgggtgcg ggcggcccgg catcgcggcg
     1801 cccggaagga gacccgagct ctcctcgagg ggctgtgcgc ggtcatggag gacaagcccc
     1861 tgatcgaatg cgccgaccac gcggtgatcc gcctggagta ccggctgcgc gatcgcgccc
     1921 gcgagcgccc ggtggccggg gtggtcaccc ccgagagcgt ggagccaggc ttcgcgcccc
     1981 tcggtgcggc ggtgcgcgag ctgctcgccg agtaccggcg ccgcaccgga tacgacgaga
     2041 ccgagaaccg cttcgatccc gagccctccg cgggctggaa ggcgctcacc cccgagcagc
     2101 ggctcgcccg catccaggtc gccctcgacg gcctcgtggc ggggcggggg ctccggtcgg
     2161 gcgacgtggt ctgcacgcgc gtcgatcgca gcacccgggc gaccgtggag ttccgcgccg
     2221 agctgcccgc gcgcgggaag gccgccctga tgatggacct cgagcggggc ctcaaggagg
     2281 cggtcgagcc ggtgctgcac ctgtaccagg aagagatgca cgacaagaac aagatccggc
     2341 ggctctgaga agggggtgac cgacacacca tgagtcaggc gttgaccgac cacaccgacc
     2401 tcatcctgga cggcaccaag atccagtggc acctggaccg ggtgcgggcc tgggagcggg
     2461 gcgagcgcat cgcgccgatc accatcgaca tggcgctgac ccgcgcctgc aactacgcgt
     2521 gccacttctg ctacgcgatg ctccaggaga acgaccgtca gacgatcacc aaggaagtga
     2581 tcaccagctt cctcgacgac tgcgcggaga tcggggtcaa gggcatcagc ctggtctcgg
     2641 acggggagag cacgatctcc ccggtcttcg tggacgcggt ggtgcacggc agcgaggtgg
     2701 gcctctcgat ggcctgcggc accaacggct tcatcttcaa ccgccgcaag ctcgaggagg
     2761 tcctgccgca cctgacctac ctgcgcatca acatctcggc gggcgagcgc aagcgctacg
     2821 cggagatcat gggggtccgg gaggaatggt ttgatcgggt agtccagaac atccgcgaca
     2881 tggtggacat caagaagcgg gagagcctgc cggtcaccat cggtctgcag atggtcctgg
     2941 acccgcggta cggcgaccag atcatgcccc tggcgcgcct gggcaaggag ctccgccccg
     3001 actacctggt gatcaagcac tgcagcgatg acgaggacgg caccctcggg gtcgactacg
     3061 aggcctacga tcgcctctac gacaccctga aggaggccga ggccctcgcc gactccgaat
     3121 acgcggtcaa ggtgaagtgg tcgaagatcg aggccaaggg ccggcgcagc taccagcgct
     3181 gctacgggcc cccgttcatg
//
LOCUS       DASUFA010000417         5145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1698912, whole genome shotgun sequence.
ACCESSION   DASUFA010000417 DASUFA010000000
VERSION     DASUFA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698912"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VFX87_15105"
     CDS             <1..1390
                     /locus_tag="VFX87_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX4996310.1"
                     /translation="NLPARVIAGGCNGMCWAAPVVTVLRGDETPRVIPRVTADRTPAL
                     LDALSAGALPAHADVTAFLTGQRRELMSRCGITDPGDIADAIQRGSYEALRRALGEAQ
                     PERVIETIKRAGLRGRGGAYFQAAIKWDGVRRAAGPIKYLIVNGEEGEPGIFKDRHLL
                     EGDPHRLLEGALLAAYAAGATRVILYIHGEAHLAARHVERALDQARAAGFVGQRVLGS
                     DFSVVVEIRRGAGGFVLGEETALMESIEGRRAMPRPKPPFPTEAGVFGRPTVINNVET
                     LFTVPLILGRGAEWWTGLGRGHGTKVFGLSGHVARPGVVEAELGLTLRDLLERMGGGA
                     PDARGVTAVALGGPSGILVPPRRFDEPLVPGGNPNPGTGGVAALHAGVPVAEAVRVLL
                     DFNTRESCGKCTPCREGTARLRDMLGDRAAVDRQAVAELADVVRVASLCGLGQAAPLS
                     ILAALSEFPGEL"
     gene            complement(1517..2665)
                     /locus_tag="VFX87_15110"
     CDS             complement(1517..2665)
                     /locus_tag="VFX87_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX4996311.1"
                     /translation="MTTAAYKDGLEDIIAARSAICRVDGEAGRLYYRGYEIADLVGAA
                     SFEDTTRLLWVGELPSAAERVEFAARLSETRPLPAPVLDLLRRLPRECHPLDALRTAV
                     SLAAASDPDVRSDDPAANLRKAYRLMNLVPGTVAAWQRIRTGREPVAARPDGSHAANF
                     LHMLDGKEPSAEVARVLDVILTLHADHEFNASTFAARVAVATVADMHSAVVAAISTLK
                     GPRHGGANEDVLAMLLEIAEPERAEAFVEARIGARAALSKRDRANPKTRMPGFGHRVY
                     KVDDARARVLRGMAKSMAEATGRGKLFEVAERLYDAMKARTSLPVNVDFFSAVVYDAL
                     GIPPDFCTSIFAVGRVAGWCAHIMEQYADNRLIRPRADYIGQPARALP"
     gene            2762..3043
                     /locus_tag="VFX87_15115"
     CDS             2762..3043
                     /locus_tag="VFX87_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4996312.1"
                     /translation="MGAMVVIEGEEYLTGDEAAGLLGVKRETLYAYVSRGLLKSYRQG
                     IKRQRLYRRVEIEALLRLAPSIAVDPATAPEGRGAQPVEVPLAESWIRE"
     gene            complement(3053..3253)
                     /locus_tag="VFX87_15120"
     CDS             complement(3053..3253)
                     /locus_tag="VFX87_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996313.1"
                     /translation="MGVYAELRGFVLAHRECGVLRGATKPIDRGFRLAVVCPCGARFL
                     RSVYAEDPEAERLQEALAAFQS"
     gene            3341..>5145
                     /locus_tag="VFX87_15125"
     CDS             3341..>5145
                     /locus_tag="VFX87_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4996314.1"
                     /translation="MNATTARVTVNESPVEVPEGATVLDAVNRLGIPLPQLCKDPDRA
                     PLGACRTCLVHVEGQRGVPAACHLPARDGMVVSTIHPEAVRARRAVLDLTLSMLTPPA
                     AGDGFGQLGAAAASHDLPHPGHAAHHRFETDASKSFFVLDREACILCGRCTVACGDVQ
                     LIGAISLVGRGHDARVGVFGDGAMASSVCTSCGQCVATCPTGALRPKEEPSPIVREVE
                     TTCPYCGVGCGITLRVRADERLSIMADDVPANRSSRGTLCVKGRFATGFVHSKDRITT
                     PLIKLDGRWQEASWDEALDAAADGLARNRGHFGALASAKATNEDGFVIQKLTRVVMGT
                     NNVDHCTRLCHSPSVEAMLVSMGSGATSNSYQDYEEAGCLMVVGADASANHPVIAIRL
                     RRAVARGARLIVVNPKRVELCDQADLWIRQQPGTDVALFNAMARVILDEGLADLDFVR
                     SRTEGFDAWVRSLAPYTLEHAEAVTGVTREDIAQAARWYARPPFVGSCLIWGMGITQH
                     INGTANAHAVLNLSLAAGQMGFPGSGISPLRGQNNVQGCGDAGCIPTNLPGYAGYGAG
                     DLDRFERAWGVRPPGEQGLVVTEMVEGCLDGRVRA"
BASE COUNT          694 a         1858 c         1870 g          723 t
ORIGIN      
        1 caatctcccc gcccgcgtca tcgccggcgg ctgcaacggc atgtgctggg ccgcccccgt
       61 ggtcacggtg ctgcgcggcg acgagacccc gcgcgtcatc ccgcgcgtga ccgccgaccg
      121 gacccccgcg ctgctggacg ccctgagcgc gggcgccctg cccgcgcatg ccgacgtcac
      181 cgccttcctg accggccagc ggcgcgagct gatgagccgc tgcggcatca cggatcccgg
      241 cgatattgcc gacgcgatcc agcgcggctc gtacgaggcg ctcaggcgcg ccctgggcga
      301 ggcgcagccc gagcgcgtca tcgagaccat caagcgcgca ggactgcgcg gccgcggcgg
      361 cgcctacttc caggccgcga tcaaatggga cggcgtgcgc cgggcggccg gccccatcaa
      421 gtacctgatc gtcaacggcg aggagggcga gcccggcatc ttcaaggacc gccatctcct
      481 ggagggcgat cctcaccggc tgctcgaagg cgcgctgctg gccgcctacg ccgcgggcgc
      541 cacgcgcgtg atcctctaca tccacggcga ggcgcatctg gccgcgcgtc acgtcgagcg
      601 cgcgctggac caggcgcggg ccgcgggctt cgtgggccag cgcgtgctcg gcagtgactt
      661 ctcggtcgtg gtcgagatcc ggcgcggcgc cggggggttc gtgctgggcg aggagacggc
      721 gctcatggag tcgatcgagg gccgccgcgc gatgccgcgg ccgaagccgc cgttcccgac
      781 cgaggcgggc gtcttcggcc ggcccaccgt catcaacaat gtggagacgc tgttcacggt
      841 cccgctgatc ctcgggcgcg gcgccgagtg gtggaccggc ctcggccgcg gtcacgggac
      901 caaggtgttc ggcctctccg gacacgtcgc gcgtcccggc gtggtcgagg cggagctcgg
      961 cctgacgctc cgtgatctgc tcgagcgcat gggcggcggg gcgccggatg ctcgcggggt
     1021 gaccgcggtg gcgctggggg gcccctccgg cattctcgtg cccccgcgcc gcttcgatga
     1081 gccgctggtg cccggtggca acccgaatcc cggtaccggc ggggtcgccg cgctacacgc
     1141 gggggttccg gtggccgagg cggtgcgcgt gctcctcgac ttcaatacgc gcgaatcgtg
     1201 cggtaagtgc acgccgtgcc gcgagggcac cgcgcggctg cgcgacatgc tcggcgaccg
     1261 cgcggcggtg gaccgccagg cggtggccga gctggccgac gtggtgcgcg tggcctccct
     1321 ctgcggcctc ggtcaggccg caccgctctc gatcctggcc gccctcagcg agttccccgg
     1381 cgagctctag gccccctcac cctgcgctct cccccgaggg gagagggtgt tccgagaacc
     1441 ttcaccctcc cgggagagag ggtgttcccg agaaccgtca ccccccggag ggggagaggg
     1501 cagggtgagg gggcgcctac ggcagggccc gcgcgggctg gccgatgtag tccgcgcgcg
     1561 gccggatcag gcggttgtcg gcatactgct ccatgatgtg cgcgcaccag ccggccaccc
     1621 ggcccaccgc gaagatggac gtgcagaagt cgggggggat gccgagcgcg tcgtagacca
     1681 ccgcagagaa gaagtccacg ttcacgggca ggctggtccg cgccttcatc gcgtcgtaga
     1741 gccgctccgc gacctcgaag agcttgccgc gcccggtggc ctctgccatg gatttggcca
     1801 tcccgcgcag cacccgggcc cgcgcgtcgt ctaccttgta gacgcggtgg ccgaagccgg
     1861 gcatgcgcgt cttgggattc gcgcgatccc gcttggagag ggcggcgcgc gcgccgatcc
     1921 gcgcctccac gaaggcctcg gcccgctcgg gctcggcgat ctcgaggagc atggccagca
     1981 cgtcctcgtt cgcaccgccg tggcgcggcc ccttcagcgt cgagatggcc gcgaccaccg
     2041 ccgagtgcat gtcggccacc gtggccaccg ccacgcgcgc cgcgaaggtc gaggcgttga
     2101 actcgtggtc ggcgtgcagt gtgaggatca cgtccaggac gcgcgcgacc tccgcggagg
     2161 gctccttgcc gtcgagcatg tggaggaagt tcgcggcgtg ggagccgtcg ggccgcgcgg
     2221 ccaccggctc gcggccggtc cggatgcgct gccaggcggc aaccgtgccc ggcaccaggt
     2281 tcatcagccg gtaggccttg cgcaggttgg cggcgggatc gtcggagcgg acgtccggat
     2341 cggaggcggc ggccagcgac accgcggtgc gcagcgcatc cagcgggtgg cactcccgcg
     2401 gcaggcggcg cagcaggtcg agcacgggcg ccggcagagg ccgcgtctcc gagagccgcg
     2461 ccgcgaactc cacgcgctcg gccgccgacg gcagctcgcc gacccagagg agccgcgtgg
     2521 tgtcctcgaa gctggccgcc ccgaccaggt ccgcgatctc gtagccgcgg tagtagaggc
     2581 gcccggcctc cccgtccacc cggcagatcg ccgaccgcgc cgcgatgatg tcctcgagcc
     2641 catccttgta cgccgccgtg gtcatggtct tagtcctccc tgattgatta tagttgattt
     2701 agattgatta gaccagactg agcctgacaa tgccgggccg aggggcgggg ggactgctag
     2761 catgggggcg atggtcgtga tcgagggtga ggagtacctg acgggggacg aggcggcggg
     2821 cctgctcggc gtgaagcgcg agacgctcta cgcctacgtg agccgcgggc tgctgaagag
     2881 ctaccgccag ggcatcaagc gccagcggct ctaccggcgc gtcgagatcg aggccctgct
     2941 ccgcctggcc ccctcgatcg cggtggatcc ggccacggcg cccgaggggc gtggcgccca
     3001 gccggtggaa gtgccgctcg ccgagtcctg gatccgggag taggccgggc cttcagctct
     3061 ggaaggcggc cagggcctcc tggagccgct ccgcctccgg gtcctccgcg tacacggacc
     3121 gcaggaaacg tgccccgcac gggcagacga cggccagacg gaaccctcga tcgatgggct
     3181 tggtggcgcc gcgcagcacg ccgcactcgc gatgtgccag gacgaagccc cgcagctcgg
     3241 cgtagacacc catggggtcc ggtagagtaa caaacccgga gggtcggggc acggggtggc
     3301 gtgtagaata agctcccgat cgggactgat ggggtttccg atgaacgcca ccaccgcacg
     3361 cgtcacggtc aacgaatctc cagtcgaagt gcccgagggc gccacggtgc tggatgccgt
     3421 gaatcgcctc ggcatcccgc tgccccagct ctgcaaggat ccggatcgcg cgccgctcgg
     3481 cgcctgccgg acctgtctgg tccacgtcga gggccagcgc ggcgtgccgg cggcctgtca
     3541 cctgcccgcg cgggacggga tggtcgtcag cacgatccat cccgaggcgg tgcgcgcgcg
     3601 ccgcgcggtg ctcgatctca cgctctccat gctcacgccg ccggcggcag gtgatggatt
     3661 cgggcagctc ggcgccgccg cggccagcca cgatctgccg caccccggcc acgcggcgca
     3721 ccatcgcttc gagaccgacg cgtcgaagtc cttcttcgtg ctcgaccggg aggcgtgcat
     3781 cctctgcggc cgctgcacgg tggcgtgcgg cgacgtccag ctcatcggcg ccatctcgct
     3841 ggtcggccgc ggccacgacg cgcgcgtcgg ggtcttcggc gacggcgcga tggcgtcctc
     3901 ggtctgcacc tcctgcgggc agtgcgtcgc cacctgcccc accggcgcgc tccggcccaa
     3961 ggaggagcct tccccgatcg tgcgcgaggt cgagaccacc tgcccctact gcggcgtggg
     4021 ctgcggcatc acgctccggg tccgcgccga cgagcggctc tcgatcatgg ccgacgacgt
     4081 gcccgccaac cgctcgagcc gcggcacgct ctgcgtgaag ggccgcttcg ccaccggctt
     4141 cgtccactcg aaggaccgca tcaccacacc cctgatcaag ctcgatggcc gctggcagga
     4201 ggccagctgg gacgaggccc tcgacgccgc cgccgacggg ctggcgcgta accgcggcca
     4261 cttcggcgcg ctcgcctcgg ccaaggccac caacgaggac ggcttcgtga tccagaagct
     4321 cacccgggtg gtgatgggca ccaacaacgt ggaccactgc acgcggctct gccactcgcc
     4381 ctcggtggag gccatgctgg tctcgatggg ctcgggcgcc acctcgaact cctaccagga
     4441 ctacgaggag gcgggctgcc tcatggtggt gggcgcggat gcctccgcca accacccggt
     4501 catcgcgatc cgcttgcgcc gcgcggtggc gcgcggggcg cgcctcatcg tggtgaaccc
     4561 caagcgcgtg gagctttgcg accaggcgga tctctggatc cgccagcagc ccgggaccga
     4621 cgtggccctc ttcaatgcga tggcgcgtgt gatcctggac gaggggctcg ccgacctcga
     4681 cttcgtccgc tcgcgcaccg agggcttcga cgcgtgggtc cgctcgctcg ccccctacac
     4741 gctcgagcac gccgaggcgg tcaccggcgt gacgcgcgag gacatcgcgc aggccgcccg
     4801 ctggtacgcg cggccgccgt tcgtgggctc gtgcctgatc tggggcatgg ggatcaccca
     4861 gcacatcaac ggcaccgcca acgcccacgc cgtgctcaat ctctcgctgg ccgccgggca
     4921 gatgggcttc ccgggctccg ggatctcgcc gctccgtggt cagaacaacg tgcagggctg
     4981 cggcgacgcg ggctgcatcc cgaccaatct gcccggctac gcgggctacg gcgcgggcga
     5041 cctcgaccgc ttcgagcggg cctggggcgt ccggccgccg ggcgagcagg gcctcgtcgt
     5101 gaccgagatg gtggagggct gcctcgacgg gcgcgtgcgg gcgat
//
LOCUS       DASUFA010000418         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_109696, whole genome shotgun sequence.
ACCESSION   DASUFA010000418 DASUFA010000000
VERSION     DASUFA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109696"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFX87_15130"
     CDS             complement(<1..215)
                     /locus_tag="VFX87_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4996315.1"
                     /translation="MMDVVVVGGGPAGSAAAILLAERGWSVALLDKAAFPRPKICGEY
                     LSPEAARVLDRLGVLKAVDAAGAQPLHG"
     gene            complement(212..934)
                     /locus_tag="VFX87_15135"
     CDS             complement(212..934)
                     /locus_tag="VFX87_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4996316.1"
                     /translation="MRFFPPLARAAGVLEIIDGPPVSFKDMACCMTDISRVNGLFGGR
                     MVTMVHVKRLLAALPSDRLVTVLDVGTGAGDIPRELVRWARRQGRRIRIFALDRDAAT
                     LRIAATLVRDYPEISFLRGDALSLPIRAGAVDLTISAMTLHHLEPDAGVRYLAEMDRA
                     ARVGFVVNDLVRSRLAHWVVWLITRFITRGAISRHDGPLSVRRSYTPGEVSGLCEQAG
                     LKGVDVVHHWPYFRFCAVRAKA"
     gene            complement(936..1961)
                     /locus_tag="VFX87_15140"
     CDS             complement(936..1961)
                     /locus_tag="VFX87_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX4996317.1"
                     /translation="MAAPKIAAVATATPPWRYDQATVLRMSGYDDPRRTGFFSNSLIE
                     TRHLYMDPETFTPDESVDQLNDRWRRGAVEVGKAAVARAIEQAGWQREDVDFLATTTC
                     TGRVCPSLDAQIINELGLKPTIQRVHVGDTGCASAMVALQQVSNHLRAFPGHRAVMVA
                     VEICSAAYFLDDRLESAVAHAIFADGAGAIALSGDGPGPEIVEHRTMFRSEHLGAMGF
                     EYPGGRPRVVLSKDVRRIGAGMMKEMADALLATHGLKQADIAHFVLHSAGRRVIEQVG
                     KLMELDENRLAHSRNVLQQYGNMSSATVVFVLDDLLRSREPAPGDWGLMIALGPGFAA
                     EGALLRW"
     gene            2055..2579
                     /locus_tag="VFX87_15145"
     CDS             2055..2579
                     /locus_tag="VFX87_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015628998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4996318.1"
                     /translation="MPLFSFEGKSPRVHPTAFIAPTANLIGDVTVEENASVWYNTVLR
                     ADFNPIVIRKGANVQDCAVIHVTPAGGVEIGPGATVGHLCLVHAATVGEEALVGNSST
                     LLDGVKIGVRAMVAAGSLVTPGTEIPDGVLAMGAPCKVKGPLAGTPAERWVQMNPAGY
                     QALAQRHRKGVQPA"
     gene            2576..3214
                     /locus_tag="VFX87_15150"
     CDS             2576..3214
                     /locus_tag="VFX87_15150"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF009676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX4996319.1"
                     /translation="MTPGPPRHILLTGFEPFDGDTVNPSAEVAKQLDGRVIGDCVVRS
                     VILPVQHEAARAVVAPLLQAPGLAAVMHLGLAGGRARISLERVAVNVMDYSRPDAHGL
                     VLRDVACVEGGPPGYFSTLPLRDVLAALTEEGIPAAISNTAGTYLCNDISYTTLHALD
                     ERALAIPTGFIHLPFLPSMVTSHDREEPSMDVAMMTRAVEIVLSRIAHPRGG"
     gene            3218..3763
                     /locus_tag="VFX87_15155"
     CDS             3218..3763
                     /locus_tag="VFX87_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996320.1"
                     /translation="MNSVTRTAMVLAVATGLLVGCTTAPPTQESRTALVESATLAMQD
                     MNRMDPGVEALARKGYGYALFPEVVKAGLGFGGGYGQGVVYEQGRHVGYADLTLASVG
                     LQIGGQTFSELIVFENKAAMDRFKLSPVDFTAGAAAMILQNGAAANATFIEGIAVIVR
                     PITGAMAEATVGGQQVKYVAK"
     gene            complement(3789..4865)
                     /locus_tag="VFX87_15160"
     CDS             complement(3789..4865)
                     /locus_tag="VFX87_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HEX4996321.1"
                     /translation="MSRATILTDRLGLRHPVIQAPLAGGGDTPALVAAVCEAGALGFI
                     GASYLTPGQIVEASRAVRARTARPFGINLFAPLPAPGGPLEPGLALERLAPFYLELAL
                     PRPELPVLAADSFGEQLSAALESGASVFSFTFGVLPAAALEAIKAQGMFVIGTATTVE
                     EAVALETAGVDAVVTQGSEAGGHRGTFAGAFEAGLVGTMALVPQVVDAVRVPVIASGG
                     IMDGRGIAAALALGAGAVQMGTAFLTCHEAGIPDAYKEAILRAHEDGTRLTRAFSGRP
                     ARGIVNRFMTEIDSTPESILPFPLQNALTRPLRSAAARQGRAEFLSLWAGQAVRLARR
                     QSAADLIARLARETDEAVGRLTRD"
     gene            4944..>5156
                     /locus_tag="VFX87_15165"
     CDS             4944..>5156
                     /locus_tag="VFX87_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4996322.1"
                     /translation="MGNIDLTALEIFKTVAEQGGIAKAATRLHRVPSNVTTRVKQLEE
                     RLGTKLFLRGNRRLVLSAEGKRLLGYA"
BASE COUNT          790 a         1906 c         1750 g          710 t
ORIGIN      
        1 ccgtggagcg gctgcgcgcc cgccgcgtcc accgccttca ggacgcccag gcgatccagc
       61 acccgcgcgg cttcgggcga gaggtactcg ccgcagatct tcgggcgcgg gaacgcggcc
      121 ttgtcgagca gggcgaccga ccacccgcgc tcggccagca ggatggcggc cgcgctcccc
      181 gccggcccgc cgccgacgac cacgacgtcc atcatgcctt ggcccggacc gcgcagaagc
      241 ggaagtacgg ccagtggtgc accacgtcca cgcccttgag cccggcctgc tcgcagagcc
      301 cgctcacctc gcccggcgtg tacgaccgcc tcacggacag cggcccgtcg tggcgcgaga
      361 tcgcgccgcg ggtgatgaac cgcgtgatca gccacaccac ccagtgcgcc agacggctcc
      421 gcaccagatc gttcaccacg aagcccacgc gcgcggcccg atccatctcg gccaggtacc
      481 gcacgcccgc gtccggctcc aggtggtgga gcgtcatcgc ggagatggtg agatccaccg
      541 cgcccgcacg aatcggcagc gagagcgcat cgccccggag gaagctgatc tcgggataat
      601 cgcgcaccag cgtcgccgcg atccggagcg tcgccgcgtc gcggtccagc gcgaagatgc
      661 ggatgcgccg gccctgccgc cgcgcccagc gcaccagctc gcgcgggatg tcccccgccc
      721 cggtgccgac gtcgagcacg gtgacgagcc ggtcgctggg caacgccgcc agcaggcgct
      781 tgacgtggac catcgtgacc atgcggccgc cgaacagtcc gttgacccgc gagatgtccg
      841 tcatgcagca cgccatgtcc ttgaaggaca cgggcggccc gtcgatgatc tcgagcacac
      901 ccgcggcacg cgccagcggc ggaaagaagc gcatgctacc agcgcagcag cgcgccctcg
      961 gccgcgaagc ccggcccgag cgcgatcatg aggccccagt cccccggcgc cggctcgcgc
     1021 gagcgcagga ggtcgtcgag cacgaagacc acggtggcgg aggacatgtt cccgtactgc
     1081 tgtagcacgt tgcgcgagtg cgcgagccgg ttctcgtcca gctccatcag cttgcccacc
     1141 tgctcgatca cccggcggcc cgcggagtgc agcacgaagt gcgcgatgtc tgcctgcttg
     1201 agcccgtgcg tggccagcag cgcgtcggcc atctccttca tcatgccggc gccgatgcgc
     1261 cgcacgtcct tggagagcac cacccgcggc cgcccccccg gatactcgaa gcccatcgcg
     1321 cccaggtgct cggagcggaa catcgtgcgg tgctcgacga tctccggccc cggcccgtcc
     1381 ccgctgagcg cgatggcccc cgccccgtcg gcgaagatgg cgtgcgccac cgcgctctcg
     1441 agccggtcgt ccaggaagta ggccgccgag cagatctcca ccgccaccat caccgcgcga
     1501 tgcccgggga acgcgcgcag gtggttggac acctgctgca gcgccaccat cgcgctggcg
     1561 cagccggtgt cgcccacgtg cacgcgctgg atggtcggct tgagcccgag ctcgttgatg
     1621 atctgcgcgt cgaggctcgg gcacacgcgc ccggtgcagg tggtggtggc gaggaagtcc
     1681 acgtcttccc gctgccagcc cgcctgctcg atcgcccgcg ccaccgcggc cttgccgacc
     1741 tccaccgccc cccgtcgcca gcggtcgttg agctggtcca cgctctcgtc gggcgtgaac
     1801 gtctccgggt ccatgtagag gtggcgggtc tcgatcaggc tattgctgaa gaagcccgtg
     1861 cgacgcgggt cgtcgtagcc cgacatgcgc agcacggttg cctgatcgta gcgccagggg
     1921 ggcgtcgcgg tcgcgacggc ggcgatcttc ggcgcggcca tgatggcgca ggctacaccc
     1981 cgcccccttg ccttgtctag gcgacgggcg caatatcgcc tcgcagccaa cccctcgtcg
     2041 tcaaggagcc gcggatgccg ctcttctcgt tcgaaggcaa gtcgccgcgc gtgcacccga
     2101 ccgccttcat cgcccccacc gccaacctga tcggcgacgt caccgtcgag gagaacgcct
     2161 cggtctggta caacaccgtc cttcgcgccg atttcaaccc gatcgtgatc cgcaagggcg
     2221 ccaacgtgca ggactgcgcg gtgatccacg tcaccccggc gggcggtgtc gagatcggcc
     2281 ccggcgccac cgtcggccat ctgtgcctgg tccacgccgc caccgtcggc gaggaagccc
     2341 tggtcggtaa ttcctccacg ctgctcgacg gcgtcaagat cggcgtgcgc gcgatggtgg
     2401 cggccggctc gctggtgacg ccgggcaccg agatccccga cggcgtgctg gcgatgggcg
     2461 ccccctgcaa ggtgaagggc ccgctcgcgg gcaccccggc cgagcgctgg gtgcagatga
     2521 acccggccgg ctatcaggcc ctcgcccagc gccaccgcaa gggagtgcag ccggcgtgac
     2581 ccccggtccg ccccgccaca tcctcctcac cggcttcgag cccttcgacg gcgataccgt
     2641 caacccgtcc gccgaggtgg ccaagcagct cgacggccgg gtgatcggcg actgcgtggt
     2701 gcgcagcgtg atcctcccgg tccagcacga ggcggcgcgc gcggtggtgg ccccgctgct
     2761 gcaggcgccg gggctggccg cggtgatgca tctgggcctc gcggggggac gggcgcgcat
     2821 ctcgctcgag cgcgtggcgg tcaacgtgat ggactactcg cggccggacg cgcacggact
     2881 ggtgctgcgc gacgtggcgt gcgtggaggg cgggccgccg ggctacttca gcacgttgcc
     2941 cctgcgggac gtgctggccg cgctgaccga ggagggcatt ccggcggcga tctccaacac
     3001 cgccggcacc tacctctgca acgacatctc ctacaccacg cttcatgccc tcgacgaacg
     3061 ggcgctcgcg atcccgaccg gcttcatcca cctcccgttc ctgccctcga tggtgacctc
     3121 gcacgatcgc gaggagccga gcatggacgt ggcgatgatg acccgcgccg tcgagatcgt
     3181 cctgtctcgc atcgctcacc caagaggagg atgacagatg aactccgtca cccggaccgc
     3241 catggtcctt gccgttgcca ccggattgct ggtcggctgc accaccgcgc caccgaccca
     3301 ggagagccgc actgcgctgg tagagagcgc gacgcttgcg atgcaggaca tgaaccgcat
     3361 ggatcccggg gtggaggccc tggcgcgcaa gggctacggg tacgccctgt tcccggaggt
     3421 ggtcaaggcc gggctgggtt tcgggggcgg ctacggccag ggggtcgtgt acgagcaggg
     3481 gcggcacgtc ggctatgccg acctgaccct ggcgtcggtc ggcttgcaga tcgggggtca
     3541 gaccttcagc gagctgatcg tgttcgagaa caaggccgcc atggaccgct tcaagctgag
     3601 cccggtcgat ttcaccgccg gcgccgcggc gatgatcctg cagaacggcg cggccgcgaa
     3661 cgccaccttc atcgagggga tcgccgtcat cgtcaggccg atcacgggcg cgatggcgga
     3721 agcgacggtc ggcggccagc aggtcaagta cgtcgcaaag tgagcggcct ccccgagcgt
     3781 cgctcgcctc aatcgcgcgt cagtcggccc acggcctcgt cggtctctct cgccaggcgc
     3841 gcgatgagat ccgcggcgga ttgccgtcga gccagccgga cggcctgccc ggcccagagc
     3901 gacaggaact ccgcgcgtcc ttgcctggcc gccgcgctcc gcaacggccg cgtgagcgcg
     3961 ttctgaagcg ggaagggcag gatggactcg ggggtgctgt cgatctcggt catgaagcgg
     4021 ttcacgatcc cgcgcgccgg gcggcccgag aaggcccgcg tcagccgcgt cccgtcctcg
     4081 tgcgcccgca ggatcgcctc cttgtacgca tcgggaatcc cggcctcgtg gcaggtcaga
     4141 aaggccgtgc ccatctgcac cgcgccggcg ccgagggcga gggccgccgc gatcccgcgg
     4201 ccgtccatga ttccaccgga cgcgatcacg ggaacgcgca ccgcgtcgac cacctgcggc
     4261 acgagcgcca tcgtgcccac caagcccgcc tcgaaggcgc ccgcgaaggt gccccgatgc
     4321 ccgcccgcct cgctgccctg cgtgacgacg gcatccacgc ccgccgtctc cagcgcaacc
     4381 gcctcctcca cggtcgtcgc ggtcccgatc acgaacatcc cctgagcctt gatcgcctcg
     4441 agagccgccg ccggcagcac cccgaacgtg aagctgaaga ccgatgcgcc ggactcgagc
     4501 gcggcggaga gctgttcgcc gaacgaatcg gccgcgagca ccgggagctc tggacgaggc
     4561 aacgcgagct ccagatagaa cggcgccaga cgctcgaggg ccagccccgg ctcgagcggc
     4621 cctcccgggg cggggagcgg cgcaaagagg ttgatcccga aggggcgcgc cgtccgggcg
     4681 cgcaccgccc gcgacgcttc gacgatctgc ccgggggtca gataggacgc gccgatgaag
     4741 ccgagggccc ccgcctcgca caccgccgcg accagcgccg gggtatcccc gccgccggcc
     4801 agcggcgcct ggatcaccgg gtgccgcagg cccagcctat cggtcaggat agtcgcgcgt
     4861 gacatgacgc acctccctgc ccgcagtctc ggccgctccg tctcttcaga aaagtgaatt
     4921 attgtgatag gactgttctc gatatgggaa acatcgacct gaccgcgctc gagatcttca
     4981 agacggtcgc cgagcagggc ggcatcgcga aggcggcgac ccggctccac cgcgtgccgt
     5041 cgaacgtgac gacgcgagtg aaacaactcg aggagagact tggcaccaag ctattccttc
     5101 gcgggaaccg gagactcgtg ctctcggcgg aggggaagcg cctgctcggg tacgcc
//
LOCUS       DASUFA010000419         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1699728, whole genome shotgun sequence.
ACCESSION   DASUFA010000419 DASUFA010000000
VERSION     DASUFA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699728"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..853
                     /locus_tag="VFX87_15170"
     CDS             344..853
                     /locus_tag="VFX87_15170"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4996323.1"
                     /translation="MTETSRSQYRTEIREISARHGVDATLVESVIRAESAFNPTAVSR
                     TGARGLMQLMPKTAVMLGVRDSFNPRENIDGGVRHLRYLLDRYPGNVALAVAAYNAGE
                     GAVDSYRGIPPYAETQQYVQRVLNGGGGGVWTGGRSVIPRSVYRYTGPNGSVTYSNLP
                     PGSRLTVAR"
     gene            complement(850..1539)
                     /locus_tag="VFX87_15175"
     CDS             complement(850..1539)
                     /locus_tag="VFX87_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4996324.1"
                     /translation="MDTPDLLARIPYFRVLSAPQRAALARRLRSVAFAPRQRVFEEGA
                     PCAGLWIVAEGRIKVFRTSPQGREQVLHVEGPGATLGEVPLFDRSGYVASATAQTAAR
                     LLWLPQRELDALCRRHPEVALAIIATLARRVRTFASLAGDLTLRSIRARLGRLVLDEV
                     GRAGRPTREGVEITLPGSRDEIAARVGTVREPLSRALAALAREGLVTVRGRRLIVRDV
                     KGLEETAGGRG"
     gene            complement(1572..2003)
                     /locus_tag="VFX87_15180"
     CDS             complement(1572..2003)
                     /locus_tag="VFX87_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996325.1"
                     /translation="MPRYFDVRTTVLLVVGYGILAEEEDRPIAYELKRAINSRSEGAD
                     NRSAVVVTDMWMLNQEMAEMFPAVGVGGPAVNAFTAQIYEDLPIAFTRDQRVFIQMNQ
                     DRGKRAALWGMDQRSTREAAELFAKDGLLDRFLELVWPRPA"
     gene            complement(2003..2581)
                     /locus_tag="VFX87_15185"
     CDS             complement(2003..2581)
                     /locus_tag="VFX87_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX4996326.1"
                     /translation="MSHAATPHPMTLDRERTALVVIDVQERLFPAMDADHREEVMRNI
                     KVLTATARRLNLDTLVTEQYPKGLGHTLQEVKDALPAGVQPVEKVAFSCLGVDAFRSR
                     LSATGARQLLLAGIEAHVCVLMSALDLLAEGYAVHIVADAVTSRTQANWRLAMAQLRQ
                     AGAVVTTTETVLFQLLRQADTDDFRELARLIR"
     gene            complement(2683..>3802)
                     /locus_tag="VFX87_15190"
     CDS             complement(2683..>3802)
                     /locus_tag="VFX87_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996327.1"
                     /translation="WEAFKSDLAFFGPRIDAWPELLVDRGYNDPPPRALLLHLLVGHL
                     PASAPALALLSSLDYVLMIAAAVAVWRAFGAVPTALTLTFLSLSFFARFDYVGGSLLR
                     WDWVAALLAGAAALARGSGTTGGLLLGYAAAARVFPVLFLMPLAIKWAQARLGRRPEP
                     AVASCLRAAFGLLLVTSAGLIAWTDQRLLVLDFLAKIRLHAQDPFVNSVGLGPILVFG
                     SVPWSSTAAGAVYVPAEAIAAARPAAALLPLLSAGYLLATLPLILRARPLESMTFAVP
                     LVFCAVSLTGYYYSFLALLVLLPWHEGRADRISAVEMALLSVIMAAAYAFELASAELL
                     PLFYQASIQLGLFLVLWTIFEYVRLGSRSRTPLTILPD"
BASE COUNT          629 a         1320 c         1355 g          498 t
ORIGIN      
        1 gaagagccga tacctcggga caaccgtgtc cgggagtcgc tgctccaggc ccgaaactga
       61 cctgtaagta actagaatat aagactgaga ggccgcccat gccgggcggc ctctcgcgtt
      121 tcaggggcct ccgtggcacg ccccgtggat atagggcgtg ctcaatcgag gtgtgcttgc
      181 gctcgtagca gcactcgccg ggctcgggac cgtatccgga gctcaggccg acaccttccg
      241 gatggtcgac ggctccggcg gggtgcatct caccaatgcc cctgcggatc cccgctatcg
      301 tggccttccc gtcgtgtcgg gtacggccac cggctggctc cgcatgaccg agacctcccg
      361 tagccagtac cgcaccgaga tccgggagat ctccgcgcgc cacggcgtgg acgcgaccct
      421 cgtcgagtcc gtgatccggg ccgagtcggc gttcaatccc accgcggtgt cgcgcaccgg
      481 cgcccgcggg ctgatgcagc tgatgcccaa gaccgcggtg atgctgggtg tccgtgattc
      541 gttcaacccg cgcgagaaca tcgacggcgg ggtgcgccac ctgcgctatc tgctggaccg
      601 ctatcccggc aacgtcgcgc tcgcggtcgc ggcctacaat gccggtgagg gcgcggtgga
      661 cagctaccgc ggcatcccgc cgtacgcgga gacccagcag tacgtgcagc gagtgctgaa
      721 cggtggcggc ggcggggtgt ggaccggtgg gcgcagcgtg atcccgcgct cggtgtaccg
      781 ctacacgggc cccaacggct cggtcacgta cagtaacctg cccccgggca gtcgcctgac
      841 cgtcgcccgc tagccgcgcc cgcccgctgt ctcctccaga cccttcacgt cccgtacgat
      901 caatcgtcgc ccgcgcaccg tcaccaggcc ttcgcgcgcc agcgccgcga gcgcgcgcga
      961 gaggggctcg cggaccgtcc ccacgcgtgc cgcgatctcg tcgcgactgc ccggcagcgt
     1021 gatctccacg ccctcccggg tggggcgccc ggcccggccc acctcgtcca gcacgagacg
     1081 gcccaggcgc gctcggatcg accggagcgt cagatcgccg gccagactcg cgaacgtccg
     1141 cacgcgtcgc gccagggtgg cgatgatggc gagggcgacc tcgggatggc gccggcacag
     1201 ggcgtcgagc tctcgctgcg gaagccagag gagccgtgcc gcggtctgcg ccgtcgccga
     1261 cgccacgtag cccgagcggt cgaacagcgg cacctcgccc agcgtggcgc ccgggccctc
     1321 gacatggagc acctgctcgc ggccctgagg ggacgtgcgg aagaccttga tccgcccctc
     1381 cgccacgatc cacaggccgg cacacggcgc gccttcctcg aacacccgct gtcgcggcgc
     1441 gaaggcgacc gagcggaggc gccgggccag cgcggcccgc tgcggagccg agaggacacg
     1501 gaagtagggg atgcgggcca gcagatccgg cgtgtccacg cgcgcgccca gcggggcggc
     1561 cgtcggccgg atcaggcggg tcgcggccag accagctcga ggaagcggtc gaggagccca
     1621 tctttcgcga acagctccgc cgcttcccgt gtgctccgct gatccatgcc ccacagcgcg
     1681 gcccgcttgc cgcggtcctg gttcatctgg atgaagacac gctggtcccg cgtgaaggcg
     1741 atcggcaggt cctcgtagat ctgggcggta aacgcgttga ccgcgggccc gccgactccg
     1801 acggcgggaa acatctcggc catctcctgg ttgagcatcc acatgtcggt caccaccacc
     1861 gcgctccggt tgtccgcccc ttcgctgcgt gagttgatcg cgcgcttgag ctcgtaggcg
     1921 atcgggcgat cctcctcctc ggcgaggatg ccgtagccga ccaccaggag caccgtggtc
     1981 cggacgtcga agtagcgggg catcagcgaa tcaggcgcgc cagctctcgg aagtcgtcgg
     2041 tgtccgcctg acgcaggagc tggaagagca cggtctcggt ggtggtgacc accgcaccgg
     2101 cctgccggag ctgggccatg gccaggcgcc agttggcctg ggtgcgcgag gtgaccgcgt
     2161 cggccacgat gtgcaccgcg tagccctcag ccagcaggtc gagcgccgac atcagcacgc
     2221 acacgtgggc ctcgatgccg gccaggagga gctggcgcgc gcccgtcgcg gagagccggc
     2281 tgcggaacgc gtccactccc aggcaggaga acgcgacctt ctcgaccggc tgcacccccg
     2341 ccggaagcgc gtccttgacc tcctgcaggg tatggccgag gcccttcggg tactgctcgg
     2401 tgaccagggt gtccagattc aaccgccgcg ccgtcgcggt caggaccttg atgttgcgca
     2461 tgacctcttc ccgatggtcg gcgtccatgg cggggaacag gcgctcctgc acgtcgatca
     2521 cgaccagtgc ggtgcgctcg cggtcgagcg tcatggggtg aggagtggcg gcgtggctca
     2581 tcgggacctc ccggagcgag gatcgaaaat cgcgcacatc ctctcacatc tggaagcgtt
     2641 ggcaagtcgt cgcgcccgtc ggcgcgccgg tgacgtgcgg gcctagtcgg gaaggatcgt
     2701 gagcggcgtc cggctccgcg agccgagccg cacgtactcg aaaatcgtcc agaggaccag
     2761 gaagaggccg agctggatcg aggcctggta gaagaggggc agcagctccg ccgacgccag
     2821 ctcgaaggca tacgcggccg ccatgatgac ggacagcagg gccatctcga cggcgctgat
     2881 ccggtcggcc cggccctcgt gccacggcaa gaggaccagg agcgcgagga aggagtagta
     2941 gtacccggtg agcgagacgg cgcagaagac cagggggacc gcgaacgtca tgctctcgag
     3001 cgggcgggcg cggagaatca aggggagggt cgccagcagg tagccggccg acagcaaggg
     3061 gagcagagcc gcggccggac gagctgccgc gatcgcctcc gcaggcacgt acacggcccc
     3121 ggccgcagtc gagctccagg gcacggagcc gaacacgagg atcgggccca ggcccaccga
     3181 gttgacgaac ggatcctggg cgtgcagccg gatcttggcg aggaaatcga gcacgaggag
     3241 tcgctgatcc gtccacgcga tgagccccgc cgacgtcacg agaaggagcc cgaacgccgc
     3301 gcggaggcat gacgccaccg cgggctcggg ccgccggccc agacgcgcct gcgcccattt
     3361 gatcgcgagg ggcatcagga acagcacggg gaacacccgg gccgccgccg catacccgag
     3421 caggagcccg cccgtggtgc cggagccgcg ggcgagcgcg gcggcgcccg cgaggagcgc
     3481 ggcgacccag tcccagcgca agagcgatcc accgacgtag tcgaatctcg cgaagaagct
     3541 caaggacagg aacgtgagcg tgagggcggt gggtacggcc ccgaacgcgc gccagaccgc
     3601 cacggccgcc gcgatcatca ggacgtagtc gagcgaggac agcagggcga gcgccggcgc
     3661 gctcgcggga aggtgtccga ccagcaggtg caggaggagc gctcgcggcg gtggatcgtt
     3721 gtatccgcga tcgacgagga gttccggcca cgcatcgatc cgcggaccga agaaggccag
     3781 gtcgctcttg aacgcctccc ac
//
LOCUS       DASUFA010000420         5301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_322777, whole genome shotgun sequence.
ACCESSION   DASUFA010000420 DASUFA010000000
VERSION     DASUFA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322777"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFX87_15195"
     CDS             <1..157
                     /locus_tag="VFX87_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4996328.1"
                     /translation="GVMLVAEGMGTHIEKGYIYFAMAFSLLVELVNMRYRRKQGAAAP
                     ARRGKEG"
     gene            complement(154..1221)
                     /locus_tag="VFX87_15200"
     CDS             complement(154..1221)
                     /locus_tag="VFX87_15200"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4996329.1"
                     /translation="MTGSEDRFYPRVFALVTAALLAGALFLILRPFLGAILWSMLLAL
                     LLFPARQALGRRLGGRYALTALLLTVATTIVLVAPLPLLAVAFASQAKELFGRVQKLA
                     AESGISGAGDVLDIPIVSRAIRWAEAVAPVDPQQIHAWLVSGAQSLLQGLVAVSGSFV
                     LGTLNALVGLFIALFVLFFLLRDGDQMMATAVRLIPITPERRGQLVDHVAAVTRAVVF
                     GSLLTALVQGALVGIGFALVGLPSPVVFGAVAAVASLVPFVGTALIWVPAVGVLFLQG
                     RWVAALFLAAWSVAVVSSADNVVRPVFISGRAQISTLPVFLGLLGGLSAFGPIGLVVG
                     PVVVALTLALLRFAEEVRGGA"
     gene            complement(1218..1934)
                     /locus_tag="VFX87_15205"
     CDS             complement(1218..1934)
                     /locus_tag="VFX87_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996330.1"
                     /translation="MMLTDAPLAVGLVFIAIVVGMLVLVLWGAARAARRVGPGAVQGV
                     RIAGVGLVGWLALTGVLAERGFFEDFQSMPPRMLLGVGLPLLTLLALTFSRRVAPLLA
                     ALPPAWPVAAQTFRILVEIVLWRLAVAGAIPELLSFHGRNVDILVGLTAPVVAYACFV
                     RRAWPARVAVWWNWAGIVILLNVVVHAQLSTPTPWRIFETDPPATFIADWPYIWLPAF
                     LVPLAWLLHAVSLRQLRSSR"
     regulatory      2096..2173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2237..2941
                     /locus_tag="VFX87_15210"
     CDS             2237..2941
                     /locus_tag="VFX87_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996331.1"
                     /translation="MRHGLIRFIVRRLAVLAIILFIPVPAAADDAILSVDQYKTAKAR
                     TLATTHQALLKQVREEIYHCMPWVGVQKAGIGFRQPKGAVEDDRYLSVWIIIDQDEDA
                     RFAALPTERRASAMFSRYGTDLLRRMAQLQPLVADPDVKGYSVVLSWLKPGTGGGSVQ
                     PVNETLALWSDKQTTLGFLAKRVPAGEFTRRAKFNVFDGVEDKGRLALEVWEDSFVRT
                     FKVANLEAPKGQRCSG"
     gene            complement(2945..3640)
                     /gene="pepE"
                     /locus_tag="VFX87_15215"
     CDS             complement(2945..3640)
                     /gene="pepE"
                     /locus_tag="VFX87_15215"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HEX4996332.1"
                     /translation="MRVFLHSGGPLGPVGRAALPDFFGPRRRVAFVTAANLDHEAAYF
                     ERVRAALEPAPPDGAGLELSHLRWDRDPLATLADAEALFMGGGNTYALLQRLRGAGLL
                     EAIRARVAAGMPYAGASAGSNVAGPTILTTNDWNVVGLDRFDALGLVGFNINPHYKEA
                     DPAMAAFSETRDDRIREYHVVNTNPVLGLEEGAWVIADQGAVTVHGTARVKLFRPAAD
                     PVWYRAGDRLPVS"
     gene            complement(3644..4435)
                     /locus_tag="VFX87_15220"
     CDS             complement(3644..4435)
                     /locus_tag="VFX87_15220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX4996333.1"
                     /translation="MSPPRRALREMRGLVFDLDGCVWTGEVLTPGAAEVLALIRRQGK
                     GLCFLTNNSRARARTMQAKLERLGVEAKEAEVLTPLEILGEVVAQRWGPSRVLAIGGP
                     ELAAVLVEAGHTLVPVDAYREATVVVVGNDFDFSYERLTAAARAVDAGAAFVTPNIDP
                     RLPMEDGQFLPGCGAIVEAVASAAGARPIVIGKPEPPLFELALQRMKLGVDDAAMVGD
                     SIDSDVRGARRVGMTAVLFAPKGGPDGVAHYIVKSMSQLKRLLGP"
     gene            4545..5189
                     /locus_tag="VFX87_15225"
     CDS             4545..5189
                     /locus_tag="VFX87_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996334.1"
                     /translation="MSNGRTYTLRNAASIVFALTAVIPLLLFTYTLYRLGQLGQFQAQ
                     MGLGVALGCALVGLFIFSNLMARLGELLRFLEGQPAPTAPPGGAAETSSAADQAARPI
                     SVATNGPATPPWPGPSGLVIPGLGTITAASRAASAARQAAGVFDEVQKTMWHAEAQQH
                     LGRRVLISVKNAPEPIAGSLAQIAEDGVLLEQDDQRVAVSYLRITNIEADPSEA"
BASE COUNT          875 a         1947 c         1855 g          624 t
ORIGIN      
        1 cggcgtgatg ctggtggccg agggcatggg cacgcacatc gagaagggct acatctactt
       61 cgcgatggcc ttctcgctcc tcgtggagct ggtcaacatg cgctaccggc gcaagcaggg
      121 cgcggccgcg ccggcgcggc gcgggaagga gggctaggcg cccccgcgca cctcttccgc
      181 gaagcgcagc agggcgaggg tcagggcgac caccaccggc cccaccacga gcccgatggg
      241 cccgaaggcg ctgagaccgc cgagcaggcc caggaacacc gggagcgtgg agatctgcgc
      301 gcggcccgag atgaagaccg ggcgcaccac gttgtcggcg ctcgacacca ccgccacgct
      361 ccacgcggcc aggaagagcg cggccaccca gcgcccctgc aggaacagca cccccaccgc
      421 gggcacccag atcagcgcgg taccgacgaa cgggaccagc gaggcgaccg cggccaccgc
      481 gccgaacacg accggcgacg gcagccccac cagcgcgaag ccgataccca cgagcgcccc
      541 ctgcacgagc gcggtcagca gcgagccgaa caccaccgct cgcgtgaccg ccgcgacgtg
      601 atccacgagc tgcccgcgcc gctccggcgt gatcgggatc agtcgcaccg cggtcgccat
      661 catctggtcg ccgtcgcgca gcaagaagaa gagcacgaag agcgcgatga acaggcccac
      721 cagcgcgttc agggtaccca gcacgaacga gccgctcacc gccaccagcc cctgcagcag
      781 cgactgggcg ccgctcacga gccaggcgtg gatctgctgg ggatccaccg gcgcgaccgc
      841 ctcggcccac cggatggcgc ggctcacgat cgggatgtcg aggacgtccc cggcgcccga
      901 gatgccggac tcggccgcca gcttctgcac ccgcccgaac agctccttcg cctggctcgc
      961 gaaggccacc gccaggagcg gcagcggcgc gaccaggacg atcgtggtgg ccaccgtgag
     1021 gaggagcgcg gtcaaggcgt agcggcctcc cagacgacgc cccagcgcct gccgcgcagg
     1081 aaagagcagg agggcgagca gcatggacca gaggatggcc cccaggaacg ggcgcaggat
     1141 caggaacagc gcgccggcca ggagcgccgc cgtcaccagc gcgaacacgc ggggatagaa
     1201 gcggtcctcg ctcccggtca tcgcgacgat cggagctggc gcagcgagac cgcgtggagc
     1261 agccaggcca gcggcaccag gaacgcgggc agccagatgt acggccagtc ggcgatgaag
     1321 gtcgcgggcg gatccgtctc gaagatccgc cacggcgtgg gcgtggagag ctgcgcgtgc
     1381 accaccacgt tcagcaggat gacgatgccg gcccagttcc accacaccgc gacgcgcgcc
     1441 ggccacgcgc gccggacgaa gcaggcatag gccaccaccg gcgcggtgag gccgaccagg
     1501 atgtcgacgt tgcggccgtg gaagctcagc agctccggga tggcccccgc caccgcgagc
     1561 cgccacagca cgatctcgac caggatgcga aaggtctggg cggcgaccgg ccaggccggg
     1621 gggagcgcgg ccaggagcgg cgcgacccgg cgggagaagg tgagggccag cagggtcagc
     1681 agcggcaggc cgacaccgag cagcatgcgc ggcggcatgg actggaagtc ctcgaagaag
     1741 ccgcgctccg ccagcacccc ggtcagcgcg agccagccga ccaggcccac gcccgcgatg
     1801 cgcacacctt gcaccgcgcc cgggcccacc cgccgcgccg cgcgcgcggc gccccagagc
     1861 acgaggacga gcatgcccac gacgatggcg atgaagacca ggccgaccgc gagcggggca
     1921 tccgtgagca tcatgcatgg atagcacatc cttcagcgaa cgcgcgagca acacgacgtc
     1981 accccggtga cacgcggggg cgctgggccg gcctatctcc ccaccggaac aggccatttc
     2041 cggccgggcg cggatggcac cgcgcttgct cctctccggg cacgcggagc ctcttcccga
     2101 caaacggcaa acccgtcgcg agacggggac gcaaagccgc ggatcaggcc gccctgacag
     2161 ccgggctacc gaaggaaggg cgctcacagt agcgctcgtc gggatccgag tgtgctccgc
     2221 gaacaggagg atcctgatga gacacggtct gattcgcttc atcgtgcgcc gcctcgcggt
     2281 gctcgccatc attctcttca tcccggttcc cgcggccgcc gacgacgcga tcctgagcgt
     2341 cgatcagtac aagacggcca aggcccgcac cctcgccacc acgcaccagg cgctgctcaa
     2401 gcaggtccgc gaggagatct accactgcat gccgtgggtg ggcgtgcaga aggcggggat
     2461 cggcttccgc cagcccaagg gcgcggtgga ggacgatcgc tacctctcgg tctggatcat
     2521 catcgatcag gacgaggatg cgcggttcgc ggcgctgccc accgagcggc gcgcctccgc
     2581 gatgttctcc cgctacggca cggacctcct gcggcgcatg gcccagctcc agccgctggt
     2641 ggccgatccc gacgtgaagg gctactcggt ggtgctctcg tggctgaagc cgggcaccgg
     2701 cggcggctcg gtgcagcccg tcaacgaaac gctcgcactc tggagtgaca agcagaccac
     2761 gctcggcttc ctcgccaagc gcgtccccgc gggcgagttc acccgccgag ccaagttcaa
     2821 cgtcttcgac ggggtagagg acaagggacg cctcgccctg gaggtctggg aggattcgtt
     2881 cgtccggacc ttcaaggtcg ccaacctcga ggccccgaag gggcagaggt gctccggcta
     2941 accgtcagga cactggtagg cggtcgccgg cccggtacca caccgggtcg gcggccggcc
     3001 ggaacagctt gacccgagcc gtgccgtgca ccgtcacggc accctggtcc gcgatgaccc
     3061 acgccccttc ctccagcccc agcaccgggt tcgtattgac cacgtgatat tcccggatgc
     3121 ggtcgtcccg cgtctcgctg aaggcggcca tggcggggtc ggcttccttg tagtggggat
     3181 tgatgttgaa gcccaccagc cccagcgcat cgaagcgatc gagtcccacc acgttccagt
     3241 cgttcgtcgt gagaatggtg ggccccgcca cgttggaccc ggcgctggcc cccgcgtagg
     3301 gcatgcccgc ggccacgcgc gcgcggatcg cctcgagcag cccggccccg cgcaggcgct
     3361 ggagcagcgc gtaggtgttg cccccgccca tgaagagcgc ttccgcgtcg gccagggtgg
     3421 cgagcggatc gcgatcccag cgcaggtgcg agagctcgag gcccgccccg tcgggcggcg
     3481 cgggctcgag cgcggcgcgc acccgctcga aatacgccgc ttcatggtcc aggttggcgg
     3541 cggtgacgaa agccacgcgg cgccgcgggc cgaagaagtc gggcagcgcc gcgcggccga
     3601 ccgggccgag cggtccgccc gagtgcagga agacgcgcat tccctacggg ccgagcaggc
     3661 gcttgagctg cgacatggac ttgacgatgt agtgcgcgac cccgtccgga ccgcccttgg
     3721 gcgcgaacag caccgcggtc atgccgacgc ggcgcgcgcc ccgcacgtcg gagtcgatgc
     3781 tgtcgcccac catcgccgcg tcgtccacgc cgagcttcat gcgctggagc gccagctcga
     3841 agagcggcgg ctcgggcttg ccgatcacga tcgggcgcgc ccccgccgcc gaggcgaccg
     3901 cctccacgat ggcgccgcag ccgggcagga actggccgtc ctccatcggc aggcgcggat
     3961 cgatgttcgg ggtgacgaag gcggcgcccg cgtccaccgc gcgcgcggcc gcggtcaggc
     4021 gctcgtagct gaagtcgaag tcgttgccca ccaccaccac ggtggcctcg cggtaggcgt
     4081 ccaccggcac gagcgtgtgg cccgcctcga ccagcaccgc ggcgagctcc ggcccgccga
     4141 tggccaggac ccgcgatggg ccccagcgct gcgccacgac ctcgcccagg atctcgagcg
     4201 gggtgagcac ctcggcttcc ttggcctcca cgcccagccg ctccagcttg gcctgcatgg
     4261 tgcgggcgcg ggcgcgcgag ttattggtga ggaaacacag ccccttgccc tgcctccgga
     4321 tcaaggcgag cacctcggcg gcacccggcg tgagcacctc gccggtccac acgcagccgt
     4381 cgagatcgaa gaccaggccg cgcatctcgc ggagggcacg ccgtggcggg ctcatgggtg
     4441 gcggtcggat actaacacgc gggccacgag cccgggggac ggcggcggca ccggctctct
     4501 gaaaactttg ggcgcgacgc cgatcgcgct agatttgaga cgccatgagt aacgggcgga
     4561 cgtatacgct gcgaaatgcg gcgtcgatcg tcttcgcgct gaccgcggtc attcccctgc
     4621 tgctcttcac ctacacgctg taccggctcg ggcagctcgg gcagttccag gcccagatgg
     4681 ggctcggcgt cgcgctcggc tgcgcgctgg tggggctgtt catcttctcg aacctgatgg
     4741 cgcgcctcgg cgagctgctg cgcttcctcg aggggcagcc ggcccccacg gcgccgcccg
     4801 gcggcgccgc cgagacgagc tcggccgccg accaggcggc tcggccaatc tcggtggcca
     4861 ccaacggccc cgcgactcca ccgtggcccg gccccagcgg cctggtcatt cccgggctcg
     4921 gcacgatcac cgcggcgagc cgggcggcct ccgcggcgcg gcaggccgcc ggcgtcttcg
     4981 acgaggtgca gaagacgatg tggcacgcgg aggcccagca gcatctcggg cggcgcgtgc
     5041 tcatctcggt gaagaacgcg cccgagccga tcgcgggcag cctggcccag atcgccgagg
     5101 acggcgtcct gctcgagcag gacgaccagc gggtggcggt cagctacctc cgcatcacca
     5161 acatcgaagc ggacccctcc gaagcctagc ggcgccgctg cacggcgccg agggcgatgc
     5221 gcgagcgcgc gtcgcccgga tccagcgcca ccgcctcgcg ataggcggcc tccgcctcgc
     5281 gccaccgctc ctggcgcgcc a
//
LOCUS       DASUFA010000421         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1249341, whole genome shotgun sequence.
ACCESSION   DASUFA010000421 DASUFA010000000
VERSION     DASUFA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249341"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1060
                     /locus_tag="VFX87_15230"
     CDS             107..1060
                     /locus_tag="VFX87_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996335.1"
                     /translation="MKRKPIPAPTAQNVFPPNLDRAYFDDAVENPFRPRAQAFEPVNA
                     WWLAEAALLAYADEGFARQQFGRAGLGEVGFVSKAGSQCYLAESRDFVIAAFRGTQVP
                     KPGATTDRARGLEETLRDFIVDADFRLVEFQPGRFVHAGFKRALESIWSELHARFLEH
                     ARARRPIWMTGHSLGAALATLAADRLGDVQGLYAFGSPAVGDRAFADGFRVNAFRIVH
                     HRDIVPRVPPFGTRPGEVQGDYVHVGALKYIDGEGRLRDEAGGTESIHGLTQEAIRSL
                     FRLSAQGRLASLLGVARDLFDDHAPLYYALRSWNCYVDSQA"
     gene            1147..2016
                     /locus_tag="VFX87_15235"
     CDS             1147..2016
                     /locus_tag="VFX87_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996336.1"
                     /translation="MKGFDGLWNAAVDRIQGERLVDQLRALDFPAALAVLGVGQAPTP
                     GPSPDLLDQVAAHVGGADGERLRHALQQLFIARKPPGEAGSRDVLIRAVQVGGGIARP
                     GRPVTPEEAVRILELLRSGEFFSDAAGTTSAVVQLTQQLPRALVRDIPRTPGRLDELG
                     EAPVRDGADAFGHSSQLAADLLDGHLDRPPSPLTNTLRWLYGNAAATAVAETVRRIIA
                     RDNETARLAILIYARAHGISLTPEGLDALHDGPLNPQDPDLGPALAAGVQALTAQRGG
                     EQGVVGVLRRLRL"
     gene            complement(2017..2748)
                     /locus_tag="VFX87_15240"
     CDS             complement(2017..2748)
                     /locus_tag="VFX87_15240"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996337.1"
                     /translation="MSPRARIGVLVPPGNPTVEPELYRMAPPGVSIHFARMQSPPSDG
                     PPGGAGGMEERTRAYREGLDGPAHALGEVRPAIVLLAHTASSYALGWGREQPLVDRVA
                     SLCRAPALLAAQAVRTALHHLGVTRLALGTPYPESISQQGRAYWEAAGFQIVGYHRLA
                     GVTDIYTESEERAAELARQADTPEAQAVLISGTGLPTVGVLERLERELGKPVISSNQA
                     CLWRALRLVGVGEPVPGFGRLLREG"
     gene            complement(2753..3352)
                     /gene="pssA"
                     /locus_tag="VFX87_15245"
     CDS             complement(2753..3352)
                     /gene="pssA"
                     /locus_tag="VFX87_15245"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX4996338.1"
                     /translation="MPGRHFSMIRSFHLADLFTIANGFCGVAALFQAMRFLVAGERWR
                     MDVATALVGLALLFDVLDGRIARWRHHASPMGRELDSLADVISFGVAPAGIAFAAGLQ
                     GALDQIILMYFVGCGLSRLARYNVTAESLASETGKVRHFEGTPIPTSIVPLGLLLLAF
                     HRQSLFPVVVGGLELHLVSLLFLLSGSLMISKTLKVPKP"
     gene            complement(3362..4555)
                     /locus_tag="VFX87_15250"
     CDS             complement(3362..4555)
                     /locus_tag="VFX87_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX4996339.1"
                     /translation="MSEPAIPGALSAVDRLVVSVVAGINMRALYGAEHPALASHVDRI
                     LEAVSAACQERQQDSLTFLVVGQDLVVETQPLRTGSLYHQQFIRALTRRSVERLTVAR
                     GLDAEECVRFLTPMALGGVPVSTRHITVGRVEMQDPSVIEQGPGPGTGAGGGPGGDSA
                     IETLTAQSVDGARDAFTEFRTDRRGGLVRLEQVVWSLMDALQRATREILPLAPLKTHD
                     DYTFVHSVNVSLLTLAQARSFGIEGARLHAIGLAAFLHDIGKLKIPLDVLNKPGKLEG
                     DEWRIMMGHAQEGATHLCGVEGSHPLAILVAYEHHMRYDGQPNYPTPRIGRRPTLASQ
                     MTSISDVYDAICTTRPYAKARSRQFAVSVLTQRVGTFHSPGLVANFVRMVGVEPGSPP
                     AESPR"
     gene            complement(4552..>5742)
                     /locus_tag="VFX87_15255"
     CDS             complement(4552..>5742)
                     /locus_tag="VFX87_15255"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX4996340.1"
                     /translation="DVDRYNPEDHRTLLAQKPTVDLDAIAVELAADPDALGPDTESDD
                     AIERRVVTTLLDMTAGTPDIVRPLVLGRLREIFVRALQQNRFTQAVGIIRAVRELTTD
                     PSLAERREALEEFLADLADAQTLAALVAASRQPGGPPFVQVQTLILALGASAARGLLE
                     ALAAEQDRGRRLRLIELAASLGAAIVPETRRLLADPRWYVVRNMVLLLRRVQDRSAMS
                     EILRCADHPDLRVRLEAIRALFAFDSKVPRDLLARTINHPDPRLAEAAVLLSGQHGIT
                     QATDLLVEILLRWDFFGRRRSMRLKALRALADLGDAAVLPRLGRFFRQWPFPLVAIEE
                     RRSAYRYLSSYDVAARAPWVARGHKSRDPVIRDICRGLAKAGPPTPSERASAGGGSAG
                     GASE"
BASE COUNT          840 a         1994 c         2107 g          801 t
ORIGIN      
        1 aatgcggacg gccgtcgtga ttagtgtgcg ctggcgcggc ggaccgcggt gagcgacttc
       61 ctgacgcggc tcggcctcgg ccagccatga cgaagaagtg acgcagatga agaggaagcc
      121 gattcccgcg ccgacggccc agaacgtgtt cccgccgaac ctggatcggg cctacttcga
      181 cgatgctgtc gagaacccat ttcggccacg ggctcaggcg ttcgaacccg tcaacgcatg
      241 gtggctggcc gaggccgccc tcctggccta cgccgacgag gggttcgcgc gccagcagtt
      301 tggacgggct ggcctgggcg aggtcgggtt cgtctcaaag gccggtagcc agtgctatct
      361 ggccgagagc cgtgacttcg tgatcgcggc gttccgcggg actcaggtgc cgaagccggg
      421 cgccaccacg gatcgcgctc gagggctcga agagacactc agggacttca tcgtcgatgc
      481 cgacttccga ctcgtcgagt tccagccggg tcgattcgtc catgccggct tcaagagggc
      541 gctcgaatcg atctggagcg agctgcacgc ccgattcctg gagcacgcgc gcgcccgtcg
      601 tcccatctgg atgacgggac acagcctcgg cgccgcgctt gccacgctcg ccgccgaccg
      661 gctcggcgac gtgcaaggcc tctacgcctt cggatctcct gccgttggtg accgcgcgtt
      721 cgccgacggg ttccgcgtca acgcgtttcg aatcgtccac caccgcgaca tcgtcccgag
      781 ggtacctccc tttgggaccc gccctggcga ggttcagggt gactacgtcc acgtgggggc
      841 gctcaagtac atcgacgggg aggggcggct ccgggacgag gcggggggca cggaatccat
      901 ccacggcctc acgcaggagg ccattcgctc gctctttcgc ctctcggctc aagggcggct
      961 cgcgagtctg ctcggggtcg cccgagacct cttcgacgac cacgcgccgt tgtactacgc
     1021 gcttcgttcc tggaactgtt acgtggatag ccaagcgtag agccggccgg tcacccagta
     1081 agggcacaaa ttatttgacg atggcgacag ggccccatca tcctcgcccc ggcaaggagg
     1141 ggcgtgatga aggggttcga cggtctctgg aacgccgcgg tcgaccggat ccagggtgag
     1201 cggctggtcg atcagctccg cgcgctggac ttccccgctg cgctcgcggt gctcggggtc
     1261 gggcaggcgc cgactccggg accttcaccc gatctgctcg atcaggtagc cgcgcacgtg
     1321 ggtggggccg atggcgagcg acttcgccac gcgctgcagc agctcttcat cgcccgcaag
     1381 ccgccggggg aagcggggag ccgggacgtt ctcatccgcg cggtccaggt gggcggcggc
     1441 atcgcccgcc cgggccggcc cgtcacgccg gaggaggccg tgaggatcct cgagctgctc
     1501 cggagcggcg aattcttcag cgacgcggcc ggtaccacca gcgcggtggt ccagctcaca
     1561 cagcagctcc cgcgggcgct ggtgcgggac atcccgagga cgcccggccg gctcgacgag
     1621 ctcggcgagg cgccggtgcg cgacggggcc gacgcgttcg gccacagcag ccagctcgcg
     1681 gccgatctcc tggacggtca tctcgaccgg ccgccgagcc cgctgacgaa taccctgaga
     1741 tggctctacg ggaacgcggc ggccaccgcg gtggcggaga cggtgcggcg gatcatcgcg
     1801 cgcgacaacg agaccgcgcg gctcgccatc ctcatctacg cgcgcgccca cggcatctcg
     1861 ctgacacccg aagggctcga cgcgctccac gacggcccgc tgaacccgca ggatcccgac
     1921 ctcggtcccg cgctcgctgc cggtgtgcag gcactgaccg cgcagcgagg cggcgagcag
     1981 ggcgtggtcg gcgtgctgcg acgcctgcgt ctctgatcag ccctccctga gcagtcgccc
     2041 gaaacccggc accggctcgc ccacgcccac cagtcgtagc gcccgccaga gacacgcctg
     2101 gttgctgctg atcaccggct tgcccagctc gcgctcgagg cgctcgagca ccccgacggt
     2161 cggcaggccc gtgccgctga tgaggaccgc ctgcgcctcg ggcgtgtcgg cctggcgcgc
     2221 gagctcggcc gcgcgctcct cgctctccgt gtagatgtcg gtcacgccgg cgagccggtg
     2281 atagccgacg atctgaaagc ccgccgcctc ccagtacgcg cgcccctgct ggctgatcga
     2341 ttccggatag ggcgtgccga gcgcgagccg cgtgacgccc aggtggtgga gcgcggtccg
     2401 caccgcctgc gcggccagga gcgcgggggc gcggcagagc gatgcgacgc ggtccaccag
     2461 cggctgctcg cgcccccagc ccagcgcgta gctggaggcg gtgtgggcca gcaggacgat
     2521 cgcggggcgc acctcgccga gcgcgtgcgc cgggccgtcc aggccctcgc gataggcgcg
     2581 cgtgcgctcc tccatgccgc cggcgccacc cggcgggccg tccgacgggg gactctgcat
     2641 ccgggcgaag tggatgctga cgccgggcgg cgccatccgg tagagttcgg gctccaccgt
     2701 cgggttcccc ggcgggacca gcacgccgat gcgcgcgcgt gggctcatgg cgctagggct
     2761 tggggacctt gagggtcttg ctgatcatca ggctgccgga gaggaggaag agcaggctca
     2821 ccagatgcag ctcgagcccg cccaccacga ccggaaacag gctctgccgg tggaaggcca
     2881 gcaggagcag gccgagcggg acgatgctgg tcgggatcgg cgtgccctcg aagtggcgca
     2941 ccttgccggt ctcggacgcg agagactccg cggtgacgtt atagcgggcg agccggctca
     3001 ggccgcagcc gacgaagtac atgaggatga tctggtcgag cgcgccctgc aggccagccg
     3061 cgaacgcgat gccggccggg gccaccccga acgagatcac gtcggcgagg gaatccagct
     3121 cgcgtcccat cggcgaggcg tgatgccgcc agcgtgcgat acgcccgtcg agcacgtcga
     3181 agagcagggc cagcccgacg agcgcggtcg ccacgtccat gcgccaccgc tcgccggcga
     3241 cgaggaagcg catggcctgg aacagggcgg cgacgccgca gaagccgttg gcgatcgtga
     3301 agagatccgc caggtgaaag ctgcggatca tcgagaagtg ccggccgggc atcgtgacgt
     3361 cctaccgggg agattcggcc ggggggctgc cgggctcgac gccgaccatg cggacgaagt
     3421 tcgcgacgag cccggggctg tggaaggtgc ccacgcgctg ggtgagcacg ctcaccgcga
     3481 actggcgcga gcgcgccttc gcgtacgggc gcgtggtgca gatcgcgtcg tagacgtcgg
     3541 agatggaggt catctggctc gccagggtgg gccggcggcc gatgcgcggg gtcgggtagt
     3601 tcggctggcc gtcatagcgc atgtggtgct cgtaggccac caggatggcg agcggatggc
     3661 tgccctcgac gccgcagagg tgcgtggcgc cctcctgcgc gtggcccatc atgattcgcc
     3721 actcgtcgcc ctccagcttg cccggcttgt tcagcacgtc gagcgggatc ttgagcttgc
     3781 cgatgtcgtg caggaaggcg gcgagcccga tggcgtgcag ccgcgccccc tcgatgccga
     3841 acgagcgcgc ctgggccagg gtgagcagcg agacgttcac cgagtgcacg aaggtgtagt
     3901 cgtcgtgcgt cttgagcggc gccaggggca ggatctcccg ggtggcccgc tggagcgcgt
     3961 ccatgaggct ccacacgacc tgctcgaggc gcacgaggcc accccggcgg tcggtgcgga
     4021 actcggtgaa cgcgtcgcgc gcgccgtcca cgctctgcgc ggtcaacgtc tcgatcgccg
     4081 agtcacctcc cgggcccccg cctgcgccgg tcccggggcc ggggccctgc tcgatcacgg
     4141 aggggtcctg catctcgacg cggcccaccg tgatgtgccg ggtggatacc ggtacgccgc
     4201 cgagcgccat cggggtcagg aagcgcacgc actcctcggc gtcgaggccg cgtgccacgg
     4261 tgaggcgctc gacgctgcgc cgggtgagcg cccggatgaa ctgctggtgg tagaggctgc
     4321 ccgtccgtag cggctgcgtc tccaccacca ggtcctgccc gaccaccagg aacgtgagcg
     4381 aatcctgctg ccgctcctgg cacgccgcgc tcaccgcctc gaggatgcgg tccacgtggc
     4441 tggcgagggc gggatgctcg gcgccgtaga gcgcgcgcat gttgatcccg gcgacgacgc
     4501 tgaccaccag ccggtcgacc gcggagagcg cgcccggaat cgccggctcg ctcattcgga
     4561 ggcacctccg gcggatccac ctccggccga cgccctttcg gacggggtgg gggggcccgc
     4621 cttcgcgagc ccgcggcaga tgtcgcggat caccgggtcc cgggacttgt ggcctcgcgc
     4681 cacccagggc gcgcgggcgg ccacgtcgta ggagctcagg tagcggtaag cggaccggcg
     4741 ttcctcgatc gccaccagag gaaacggcca ctggcggaag aagcggccaa gccgcggcag
     4801 cacggccgcg tcccccaggt cggccagggc gcggagcgcc ttgagccgca tcgagcggcg
     4861 ccgcccgaag aagtcccagc ggaggaggat ctccaccagc aggtcggtcg cctgggtgat
     4921 gccgtgctgg ccggagagca gtaccgccgc ctccgccagt ctcgggtccg ggtggttgat
     4981 ggtccgcgcg agcagatcgc gcggcacctt ggagtcgaag gcgaagagcg cccggatggc
     5041 ttcaagccgg acccggaggt ccggatgatc cgcgcagcgc aggatctcgc tcatggccga
     5101 gcggtcctgc acgcgccgga ggagcagcac catgttccgc accacgtacc agcgcggatc
     5161 cgcgaggagg cgccgcgtct ccgggacgat ggcggcgccc agcgaggccg cgagctcgat
     5221 cagccggagg cggcgccccc gatcctgctc ggcggccagc gcctcgagga gtccgcgggc
     5281 ggccgaggcg cccagggcca ggatcagcgt ctgcacctgc acgaacggcg gcccgcccgg
     5341 ctgccggctc gcggccacga gggcggccag ggtctgggca tcggcgaggt cggcgaggaa
     5401 ctcctcgagc gcctcgcgcc gctccgccag gctcgggtcc gtggtcagct cgcgcacggc
     5461 gcgaatgatg ccgacggcct gggtgaagcg gttctgctgg agcgcgcgga cgaagatctc
     5521 ccgcaggcga cccaggacga gcgggcgcac gatatcgggg gtgcccgcgg tcatgtcgag
     5581 cagcgtcgtg acgacccgcc gctcgatcgc gtcgtcgctc tcggtgtcgg gcccgagggc
     5641 gtcgggatcg gcggccagct cgaccgcgat cgcgtccaag tccacggtcg gcttctgggc
     5701 cagcagcgtg cgatggtcct cggggttgta gcggtccacg tc
//
LOCUS       DASUFA010000422        10579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_796075, whole genome shotgun sequence.
ACCESSION   DASUFA010000422 DASUFA010000000
VERSION     DASUFA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796075"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1573
                     /locus_tag="VFX87_15260"
     CDS             212..1573
                     /locus_tag="VFX87_15260"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX4996341.1"
                     /translation="MQADAKGLEALIKKGDVDTVLTVFADGLGRLMGKRVVGRYFLDH
                     VLHDGVHACIYLFTVDMEMEPLAGFKLTSWERGYGDMKLVPDLTTWRLLPWLPKTALV
                     FCDVYTEEGEPIEESPRWVLRRQLERAAKVGYRVKTASELELYLFKESFDEARAKRYH
                     GLTPVSSYLEDYHILQTSREEPLVRAIRNGMEAARVPVEFSKGEWGRGQEEINLRYAD
                     ALEMADRHVLYKHGVKEIAWQQGCSVTFMAKYDMGAAGSSFHLHSSVWDQAGRRNLFA
                     AKDGSRQGTPLFQQWLAGQMAMAREFAYFYAPTVNAYKRYQAGSFAPTRIAWGWDNRT
                     CGFRICGEGGGFRVENRIPGADANPYLAFAATIAAGLRGVAQKLRPPAMFDGNAYEDT
                     KLAQVPKTLRDALAELEGSQAAREAFGPKVVEHYLQHGRLEQQAYDQSVTDWELIRLF
                     ERI"
     gene            1629..2393
                     /locus_tag="VFX87_15265"
     CDS             1629..2393
                     /locus_tag="VFX87_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4996342.1"
                     /translation="MPGRLRNKIALITGAGMGMGREASVLFAEEGARVVVCDLNGAAA
                     AETVELVQKAGGEALAVVGDVAVEAEVRRMVEDGVRRFGALHILYNNAGVLWKDRDRS
                     VLETDGEQWDRVMAINLKSVFWVTKYGIPHLKAAGGGSIILVGSVSALAGFTRAQDAY
                     TAAKGALISLNKSLAIQFAKDNIRSNVIHPGIVETPLQAPYLNDALRAEFKTGIPLGR
                     IGRPRDIANAALFLASDESSFMTGAEMIIDGGFMAQ"
     gene            2445..3464
                     /locus_tag="VFX87_15270"
     CDS             2445..3464
                     /locus_tag="VFX87_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4996343.1"
                     /translation="MMLTVARSVLALVLTAALALAVVTPAAAQKQTVVIYTAIENEQI
                     AEYKKAYEKSLPNIEVKMLRLSTGDIAARFMAEKDNMQADVIWGVGATNMLQFKNAGL
                     LEAYAPKGLNRVQPLFRDKANPPSWVGLDIYMSAFCFNTDTGKKHNLPRPESWADLTK
                     PVYKGHVVMPNPASSGTGYLSVVSVLQRMGEAEGWKYLDALDKNIAEYTKSGSKPCKD
                     SAAGERAIGVSFEYVAHEMKKKGAPVEMVLPKEGSGYEIEANGLTKKGAKNPAARQFL
                     DWAITDESLKIAAQFFAAVAVSGMPVPEGLPKDISKVVFPNDFDWSAKNRDRILAEWQ
                     KRYAK"
     gene            3503..5455
                     /locus_tag="VFX87_15275"
     CDS             3503..5455
                     /locus_tag="VFX87_15275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX4996344.1"
                     /translation="MKLPADSPGRLTDDVDLDTALARLGYAAFRPGQREAVETLLARG
                     RLLLVAPTGGGKSLSYQLPATLLPGTTLVISPLVALMADQVQALEARGVPATYLAATL
                     DAAEIRRRMARLAAGAFRLVYVAPERLAFPGFRAMLHEISIPLIAVDEAHCISEWGHD
                     FRPEYLGIGALIGDFPSARVLACTATATPVVRDEILGRLGLPADTPQHVHGFARPNLA
                     RRAVEVDGRRERERLGDAALAEALGGPGRGGGTAIVYAPTRKKAEEETDRLVGRGWGA
                     VAYHAGLDGRTREIAQRGFAEGRVEVVVATNAFGMGIDRPDVRAVIHLGPPGSIEAYY
                     QEVGRAGRDGAPALGLLLVSGSDLPLRRALLERGADGAAPDPAVVEHKWGMFLELIRW
                     VEGGSCRHDAILRYFGDEAETLAGCGRCDVCRALEAAPEEEDAERTSLIVRKALSGVA
                     RVHERFGLQTVVKLVRGEPDERLERAGLTRVPTHGNLREHPEAWILALLRRCVSAGWV
                     SFSGRERPVVVLTEEGRAVMKGERPVRLLLPPTGRRADAAPAAARSSEPERRRPSRAA
                     AELDEAALALFEALRRHRLTVARAEGLAPFIVASDRTLRDIAVLKPRTRAELEMAHGV
                     GPHKAERYGPGLLRVVAEETARGSRG"
     gene            5452..6543
                     /locus_tag="VFX87_15280"
     CDS             5452..6543
                     /locus_tag="VFX87_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX4996345.1"
                     /translation="MSAQAARPAAVALEGIAKKFGQFVALRGITLSISPGEFICFLGP
                     SGCGKTTLLRIIAGLERQNAGVVMMGGRDVSALPPSQRNYGIVFQSYALFPNLTVARN
                     VGYGLETRHVAKAQIEARIDELLTLVGLAAHKHKYPAQLSGGEQQRIALARALAPSPA
                     LLLLDEPLSALDARVRQSLRHEIRALQRRLGVTTIMVTHDQEEALAMADRIVVMNHGV
                     VEQVGAPVEVYTRPRSPFVARFVGQMNFLAATAGERAGWARLGAVEMRHGATEPIVAG
                     TRLTLAIRPEEITVGAAVGAENRLVTRIRAIQFLGPFTRLSLALPDGAPALECDVAAT
                     AFASLGAAEGSELALSLRPEALRVFPAAG"
     gene            6550..8295
                     /locus_tag="VFX87_15285"
     CDS             6550..8295
                     /locus_tag="VFX87_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEX4996346.1"
                     /translation="MPRAAASEGLPSTIPDRVVRHRLVGEDLLRYALVAVFAIVLYLF
                     VLYPLLHVIWRSLLGADGSFVGPANYRRYFGTPAIAASIWNSLSVSLVSMVITVALAF
                     GYAYGLTRTVMPWRGLFRIVAMLPLFAPSLVQALAFIYVFGNNGIFTRATGYNVGIYG
                     AKGIVAAEVFYCFPHALLILVAALSATDARLYDAARTLGASPLKTFLTVTLPGVKFGL
                     VSACFVVFTLVITDFGAPKAIGGKFSVMATEIYNQVSGQQNFTMGATVSVVLLIPAVL
                     AFLVDRVVQRRQYALVTSSSRPLAPARRPLADWAGFGYCALIAGAIAAMYLAIAVYSL
                     VARWPYNFTPTLRHYRFDTVGGYAPLWNSVYVAALTALVGTVLTFVGAYVVEKCRTRA
                     SGVLYLLSVLPVSIPGMVLGLAYIFTFNKAGSPLNVLYGTLAILIISNVIHYFTVGFL
                     TATTALRQMDAEFENVSASLGVPFYRTFWRVTVPMALPPLVGISMYFFLNAMVTLSAV
                     VFLVAPGTELAAVAVLLMDDAGDTAQAAAMSVCIIAIGLTVRVAYWALMRGVSRRTQA
                     WREPRESTTLAPEPP"
     gene            8292..8609
                     /locus_tag="VFX87_15290"
     CDS             8292..8609
                     /locus_tag="VFX87_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4996347.1"
                     /translation="MTATLVDIHRHRGRFFEVLQETERTQTAVMTIAPGQDGGPEETH
                     AGDQVVFVVEGEAIVTVQGAAHQARAGACVLIPAGAPHHVRNPGPQPLFFLTVYAPPA
                     YSR"
     gene            8606..9343
                     /locus_tag="VFX87_15295"
     CDS             8606..9343
                     /locus_tag="VFX87_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4996348.1"
                     /translation="MTVAGKTALVTGAARGIGLAIATRLAADGARVALLDQDADAVEA
                     AARGIGRGALALAGDVTRAKDVDGAVARVESAWSRLDVLVNNAGITGRSFPIWELSDD
                     DWQRVIDVDLTSVFLCCRAAVKVMLRQGGGRIVNIASIAGKEGNPTLVPYSTAKAGVI
                     GLTKALAKEVATRGIFVNAVAPAVIGTELLKQMEQSTVDLLVSKIPMGRVGKPEEVAA
                     LVAWLASDECSFSTGAVYDLSGGRATY"
     gene            9353..10306
                     /locus_tag="VFX87_15300"
     CDS             9353..10306
                     /locus_tag="VFX87_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996349.1"
                     /translation="MSRVASWLVVVMLSAAASVQAQTRDVGAMITEIKPGRGRIEVSG
                     PGTAGWRPAGPLLALKVGDSLRATDDASAVVVLAGRRGSVQVDAARSPFVVTAPPADS
                     KMQKAFLLLESGLGFLGSNPRETPRPVLSTRSVGRAPVILSPRAGPVMGDALTFEWQG
                     YQFGRYTLRVVAGSSVVLERSGLTGSRLVYPADAPALVPGTAYTLQLFSGQSVVDEIR
                     FEVADRQRADDLRRDLREVDQALGPDVPASSRVVMQAGVLASRGFLHDARRLVVAALP
                     ADADQPSLHALLGDLYARTGLPREADEAYEQARALASPPSR"
BASE COUNT         1495 a         3717 c         3806 g         1561 t
ORIGIN      
        1 cgctcgacga cctcaccccg ctgtcgacgc gccgctacgt cctcggcgtc ttctgcctga
       61 tcctcctgat cgccctgatc ccgcccgtcc ccatccgcta gccggggtca ggtcttacat
      121 tccgacattt ccgacgctcg ccgtgtttcg cctgccgtat cgaaatgtcg aaatgcaaga
      181 cctgacccca ttgcggtaag attgccgcgc tttgcaggca gacgcgaagg gtctcgaggc
      241 gctgatcaag aagggcgatg tggacaccgt gctcacggtg ttcgccgacg gcctcggccg
      301 cctgatgggc aagcgggtcg tgggccgcta cttcctcgac cacgtcctgc acgacggcgt
      361 ccacgcgtgc atctacctgt tcacggtgga catggagatg gagccgctgg ccggcttcaa
      421 gctcacctcg tgggagcgcg gctacgggga catgaagctg gtcccggacc tcaccacgtg
      481 gcggcttctg ccgtggctgc ccaagaccgc gctggtcttc tgcgatgtct acacggagga
      541 gggcgagccg atcgaggagt cgccgcgctg ggtgctgcgg cgccagctcg agcgcgcggc
      601 caaggtcggc taccgggtca agaccgcctc cgagctcgag ctctatctct tcaaggagtc
      661 gttcgacgag gcgcgggcca agcgttacca cggcctcacc ccggtctcga gctatctcga
      721 ggactaccac atcctccaga cttccaggga agagccgcta gttcgcgcga ttcgcaacgg
      781 gatggaggcg gcgcgcgtgc cggtggagtt ctccaagggc gagtgggggc ggggacagga
      841 ggagatcaac ctgcgctacg ccgacgcgct ggagatggcc gaccgtcacg tgctctacaa
      901 gcacggggtg aaggagatcg cctggcagca gggctgctcg gtgaccttca tggccaagta
      961 cgacatgggc gcggcgggct cgtccttcca cctgcactcc tcggtgtggg atcaggcggg
     1021 gcggcgcaac ctgttcgcgg ccaaggacgg cagccggcag ggcacgccgc tgttccagca
     1081 gtggctcgcc gggcagatgg cgatggcgcg cgagttcgcg tatttctacg cgcccaccgt
     1141 caacgcctac aagcgctacc aggccggctc cttcgcgccc acccgcatcg cgtggggctg
     1201 ggacaatcgc acctgcggct tccgcatctg cggggagggc ggcggcttcc gggtggagaa
     1261 ccgcatcccg ggcgccgacg ccaaccccta cctggccttc gcggccacca tcgcggcggg
     1321 actgcgcggc gtggcgcaga agctgaggcc gccggccatg ttcgacggca acgcctacga
     1381 ggacacgaag ctggcccagg tgccgaagac cctgcgcgac gccctcgccg agctcgaggg
     1441 gtcgcaggcc gcgcgcgagg ccttcgggcc caaggtcgtg gagcactacc tgcagcacgg
     1501 ccggttggag cagcaggcat acgaccagtc cgtcaccgac tgggagctga tccgcctctt
     1561 cgaacgaatc taggcccctc accctgccct ctccccaggg ggagagggag aatcgggaga
     1621 agcgctgaat gccaggaagg ctcagaaaca agatcgccct catcaccggc gcggggatgg
     1681 gcatgggccg cgaggcctcg gtcctcttcg ccgaggaagg cgcgcgcgtc gtggtctgcg
     1741 acctgaacgg cgcggccgcc gccgagacgg tggagctggt gcagaaggcg ggcggggagg
     1801 cgctggcggt ggtgggcgac gtggccgtgg aggccgaggt gcgccgcatg gtggaggacg
     1861 gggtgcgccg cttcggggcg ctccacatcc tttataacaa cgcgggcgtg ctctggaagg
     1921 accgcgaccg gtcggtgctc gagacggacg gcgagcagtg ggaccgggtc atggccatca
     1981 acctcaagtc ggtcttctgg gtgaccaagt acggcatccc gcacctcaag gcggccgggg
     2041 gaggatcgat cattctggta ggctcggtct cggcgctggc cggcttcacg cgggcccagg
     2101 acgcctatac cgcggccaag ggcgcgctga tctccctcaa caagtcgctg gccatccagt
     2161 tcgcgaagga caatatccgc tcgaacgtga tccacccggg gatcgtggag actccgttgc
     2221 aggccccgta tctcaacgac gcgctgcggg cagagttcaa gaccggaatc cccctcggac
     2281 ggatcggccg gcctcgcgac atcgcgaacg ccgcgctgtt cctcgcctcc gacgagtcgt
     2341 cgttcatgac gggggcggag atgatcatcg acggcggatt catggctcag taaggcgtcg
     2401 atcctgacga tgtcccactc gacccgcccc ccaaggaggc gtccatgatg ctgacagtcg
     2461 cgcgcagcgt cctcgccctg gtcctgacgg ccgcgctggc cctcgccgtg gtcaccccgg
     2521 cggcggccca gaagcagacc gtggtcatct acaccgcgat cgagaacgag cagatcgccg
     2581 agtacaagaa ggcctacgag aagtcgctgc ccaacatcga ggtcaagatg ctgcggctct
     2641 ccaccgggga catcgcggcc cggttcatgg ccgagaagga caacatgcag gcggacgtga
     2701 tctggggtgt cggcgccacc aacatgctcc agttcaagaa cgcgggcctg ctcgaggcgt
     2761 acgcgcccaa ggggctcaac cgcgtccagc cgctcttccg cgacaaggcc aatccgccct
     2821 cgtgggtggg actcgacatc tacatgtccg ccttctgctt caacaccgac accggcaaga
     2881 agcacaacct gcccaggccg gagtcctggg ccgatctcac caagcccgtc tacaagggcc
     2941 acgtggtgat gcccaacccg gcgtcctcgg ggaccgggta cctctcggtc gtctcggtcc
     3001 tgcagcgcat gggcgaggcg gagggctgga agtatctcga cgcgctcgac aagaacatcg
     3061 cggagtacac gaagtcgggc tccaagccct gcaaggactc ggcggcgggc gagcgggcga
     3121 tcggcgtgtc gttcgagtac gtggcccacg agatgaagaa gaagggcgcc ccggtggaga
     3181 tggtgctccc caaggagggc tcgggctacg aaatcgaggc caacgggctc accaagaagg
     3241 gggccaagaa cccggcggcg cggcagttcc tcgactgggc catcaccgac gagtcgctga
     3301 agatcgcggc gcagttcttc gcggcggtgg cggtgagcgg catgccggtg cccgagggcc
     3361 tgcccaagga catcagcaag gtggtgttcc cgaacgactt cgactggtcc gcgaagaacc
     3421 gggaccgcat cctcgccgag tggcagaagc gctacgcaaa gtagggcgag gcgcagccga
     3481 aggcgagccg agcggataag taatgaagct tccggctgat tcgcctggcc ggctgaccga
     3541 cgacgtcgac ctcgacaccg cgctcgcgcg gctcggctac gcggccttcc ggccgggcca
     3601 gcgcgaggcg gtggagaccc tgctcgcccg cggccggctc ctgctggtgg cgcccaccgg
     3661 gggcggcaag agcctcagct accagctccc cgccacgctg ctgcccggta ccacgctggt
     3721 catctcgccc ctggtggccc tgatggccga tcaggtgcag gcgctcgagg cccgcggggt
     3781 gcccgcgacg tacctggcgg ccaccctcga cgccgccgag ataaggcgcc gcatggcgcg
     3841 gctggccgcc ggggccttcc ggctggtcta cgttgcgccc gagcggctcg cgtttcccgg
     3901 gttccgggcg atgctccacg agatcagcat cccgctcatc gcggtggacg aggcccactg
     3961 catcagcgag tggggccacg acttccgccc ggagtacctc gggatcggcg cgctgatcgg
     4021 cgacttcccg agcgcgcgcg tgctggcctg cacggccacc gcgacgccgg tcgtgcgcga
     4081 cgagatcctg ggccgcctcg ggctgcccgc ggacaccccg cagcacgtcc acgggttcgc
     4141 gcgcccgaac ctcgcccggc gcgcggtcga ggtggacggc cgccgcgagc gcgagcggct
     4201 gggggacgcg gcactcgccg aggctctcgg tgggcccggc cgcggcggcg gcactgcgat
     4261 cgtctacgcg ccgacccgca agaaggcgga ggaggagacc gaccggctgg tcggccgggg
     4321 ctggggagcc gtcgcctacc acgccgggct ggacggccgc acgcgagaga tcgcccagcg
     4381 ggggttcgca gaggggcgtg tcgaggtcgt ggtggccacc aacgcgttcg gcatgggcat
     4441 cgaccggccc gacgtccgcg cggtgatcca cctgggcccg cccggctcca tcgaggccta
     4501 ctaccaggaa gtgggccgcg ccggtcgcga cggggcgccc gccctcgggc tgctcctggt
     4561 gagcggcagc gatctcccgc tgcgccgcgc cctgctcgag cgaggcgccg acggcgcggc
     4621 gccggacccc gccgtagtcg agcacaagtg ggggatgttc ctcgagctga ttcgctgggt
     4681 cgagggtgga agttgccgtc acgatgcgat tctccggtac ttcggcgacg aggcggagac
     4741 gctcgcgggc tgcgggcgct gcgacgtttg ccgcgcgctc gaggccgccc cggaagagga
     4801 ggatgccgag cgcacctcgc tcattgtccg gaaggccctc tcgggagtcg cccgggtcca
     4861 cgagcgcttc gggctgcaga ccgtggtcaa gctggttcgc ggcgagccgg acgagcgcct
     4921 ggagcgagcc ggcctcaccc gggtgccgac tcacggcaac ctcagggagc atccggaggc
     4981 gtggatcctg gccctgttgc gccggtgcgt ctccgcgggg tgggtgagct tctcgggacg
     5041 tgagcgcccg gtggtcgtcc tgaccgagga agggcgcgcg gtgatgaagg gcgagcggcc
     5101 ggtgcggctg ctgctgccgc cgaccggccg tcgcgcggac gccgcgcccg ccgcggcgcg
     5161 gtcgtcggag ccggagcggc gccggccctc gcgcgcggcc gccgagctgg acgaggcggc
     5221 gctggcgctc ttcgaggctc tccgacgcca ccggctcacg gtggcgcggg ccgagggcct
     5281 cgcgcccttc atcgtggcca gcgatcgcac cctccgcgac atcgccgtgc tgaagccgcg
     5341 gacgcgggcc gagctcgaga tggcgcacgg ggtggggccg cacaaggccg agcgctacgg
     5401 ccccgggctc ctgcgcgtgg tggccgaaga gaccgcgaga ggaagccgcg ggtgagcgcg
     5461 caggccgccc ggcccgcggc ggtggcgctc gagggtatcg ccaagaagtt cggtcagttc
     5521 gtggccctgc gcggcatcac gctgtccatc tcgcccgggg agttcatctg cttcctgggg
     5581 ccgagcggct gcggcaagac cacgctgctg cggatcatcg ccgggctcga gcggcagaac
     5641 gcgggcgtgg tgatgatggg cgggcgcgac gtctccgcgc tgccgcccag ccagcgcaac
     5701 tacggcatcg tgttccagtc ctacgccctc ttcccgaacc tcacggtggc ccgcaacgtg
     5761 ggctacgggc tcgagacgcg gcacgtggcg aaggcccaga tcgaggcgcg catcgacgag
     5821 ctcctgaccc tggtggggct cgcggcccac aagcacaagt acccggccca gctctcgggc
     5881 ggcgagcagc agcggatcgc gctcgcgcgg gccctggccc cgtcgcccgc cctgctgctg
     5941 ctcgacgagc cgctctcggc gctcgacgcg cgcgtgcgcc agtcgctccg ccacgagatt
     6001 cgcgcgctgc agcggcggct cggcgtgacc acgatcatgg tgacgcacga tcaggaggag
     6061 gcgctggcca tggccgaccg catcgtggtg atgaaccacg gggtggtgga gcaggtgggc
     6121 gcgcccgtcg aggtctacac gcgcccgcgc tcgccgttcg tcgcgcgctt cgtcggccag
     6181 atgaacttcc tggcggccac cgcgggggag cgggccgggt gggcgcggct cggcgcggtg
     6241 gagatgcggc acggggccac cgagccgatc gtcgcgggca cgcggctcac gctggcgatc
     6301 cggcccgagg agatcacggt cggcgccgcg gtgggcgcgg agaaccggct ggtcacccgc
     6361 atccgtgcca tccagttcct gggcccgttc acccgcctga gcctggccct ccccgacggc
     6421 gcccccgcgc tggaatgcga cgtggcggcc accgccttcg cgagcctggg cgccgccgag
     6481 ggctccgagc tggcgctctc gctccggccc gaggcgctgc gcgtcttccc ggccgcgggc
     6541 tagcgtcgga tgccgcgcgc ggcggcgtcg gaagggctcc cgtccacgat cccggaccgc
     6601 gtggtccgcc accggctggt cggtgaggac ctgctgcgct acgcgctggt cgccgtcttc
     6661 gcgatcgtcc tctacctctt cgtgctctac ccgctgctgc acgtgatctg gcggagcctg
     6721 ctgggcgcgg acggcagctt cgtggggccg gccaactacc ggcgctactt cggcaccccc
     6781 gccatcgccg cgtccatctg gaacagcctc tccgtctcgc tggtgtccat ggtcatcacg
     6841 gtggcgctgg ccttcggcta cgcctacggg ctcacgcgca ccgtgatgcc gtggcgcggg
     6901 ctgttccgga tcgtggcgat gctgccgctc ttcgcgccct ccctggtgca ggcgctggcc
     6961 ttcatctacg tgttcggcaa caacgggatc ttcacccgcg ccaccggcta caacgtgggg
     7021 atctacggcg ccaagggcat cgtggcggcc gaggtgttct actgcttccc gcacgcgctc
     7081 ctcatcctgg tggctgcgct ctccgccacc gacgcgcgcc tgtacgacgc ggcgcggacc
     7141 ctcggggcct cccccctcaa gaccttcctg accgtgaccc tgcccggcgt gaagttcggg
     7201 ctggtgagcg cctgcttcgt ggtcttcacc ctcgtcatca ccgacttcgg cgcgcccaag
     7261 gcgatcggcg gcaagttctc ggtgatggcc accgagatct acaaccaggt ctcgggccag
     7321 cagaacttca ccatgggggc caccgtctcg gtggtgctgc tgatcccggc ggtgctcgcc
     7381 ttcctggtgg atcgggtggt ccagcgccgc cagtacgcgc tggtgacgtc gtcctcgcgc
     7441 ccgctcgcgc cggcccgccg tccgctcgcc gactgggccg gcttcggcta ctgcgcgctc
     7501 atcgcgggcg ccatcgccgc catgtacctg gccatcgcgg tgtactcgct ggtggcgcgc
     7561 tggccctaca acttcacgcc gaccctgcgc cactaccgct tcgacacggt gggcggctat
     7621 gcgccgctgt ggaacagcgt gtacgtggcc gcgctgaccg cgctggtggg gaccgtgctc
     7681 accttcgtgg gggcctacgt ggtcgagaag tgccgcaccc gcgcgagcgg cgtactctac
     7741 ttgctgtcgg tcctgccggt atccatcccg ggcatggtgc tgggcctggc ctacatcttc
     7801 acgttcaaca aggcgggcag cccgctcaac gtgctctacg gcaccctggc gatcctgatc
     7861 atctcgaacg tcattcacta cttcacggtg ggcttcctca ccgccaccac cgcgctccgg
     7921 cagatggacg cggagttcga gaacgtctcg gcctcgctgg gcgtgccctt ctaccggacg
     7981 ttctggcggg tgacggtgcc gatggccctg ccgcccctcg tgggcatcag catgtacttc
     8041 ttcctcaacg cgatggtgac gctctccgcg gtggtcttcc tggtggcgcc cggcaccgag
     8101 ctggccgcgg tggcggtgct gctgatggat gacgcgggcg acaccgccca ggccgccgcg
     8161 atgtcggtgt gcatcatcgc catcgggctg accgtgcgcg tggcctactg ggccctgatg
     8221 cggggcgtga gccggcgcac ccaggcctgg cgcgagcccc gagaaagcac tacgctagcc
     8281 cccgagcccc catgaccgcc accctggtcg atattcaccg ccaccgcggg cgcttcttcg
     8341 aggtgctgca ggagaccgag cgcacccaga ccgcggtcat gacgatcgcg cccggccagg
     8401 acggcggccc cgaggagacc cacgcgggcg accaggtcgt cttcgtggtg gagggcgagg
     8461 cgatcgtgac cgtccagggg gccgcgcacc aggcgcgcgc cggggcatgc gtgctgatcc
     8521 cggcgggcgc gccccatcac gtgcggaacc cgggcccgca gccgctgttc ttcctcacgg
     8581 tctacgcgcc cccggcctac tcgcgatgac cgtcgccggc aagaccgcgc tcgtcaccgg
     8641 agcggcccgc gggatcgggc tcgcgatcgc cacccgcctg gccgccgacg gcgcgcgggt
     8701 ggccctgctc gaccaggacg ccgacgcggt ggaggcggcg gcgcgcggca tcggccgcgg
     8761 agccttggcg ctggccgggg acgtgacccg ggccaaggac gtggacggcg ccgtcgcccg
     8821 cgtggagagc gcctggagcc gcctcgacgt gctggtcaac aacgcgggga tcaccggccg
     8881 ctcgtttccg atctgggagc tctccgacga cgactggcaa cgcgtcatcg acgtggacct
     8941 caccagcgtg ttcctctgct gccgggccgc ggtgaaggtg atgctgcggc agggcggggg
     9001 ccgcatcgtc aacatcgcgt cgatcgcggg gaaggagggc aatccgacgc tcgtgccgta
     9061 ctccaccgcc aaggccggcg tgatcgggct gaccaaggcg ctcgccaagg aggtggcgac
     9121 ccgcgggatc ttcgtcaacg cggtggcgcc cgcggtgatc ggcaccgagc tgctcaagca
     9181 gatggagcag agcacggtgg acctgctggt ctccaagatc ccgatgggcc gcgtggggaa
     9241 gccggaagag gtggcggcgc tggtggcgtg gctcgcctcg gacgagtgct ccttcagcac
     9301 cggcgccgtc tacgacctct cgggggggcg cgcgacgtac taggaggtga gcatgagccg
     9361 agtcgcgtcc tggctggtgg tcgtgatgct gagcgcggcg gcctcagtgc aggcccagac
     9421 gcgggacgtg ggtgccatga tcaccgagat caagccgggc cgcgggcgga tcgaggtgag
     9481 cgggccgggg acggcgggct ggcggcccgc ggggccgctg ctggcgctca aggtgggcga
     9541 cagcctccgc gcgacggacg acgcatcggc ggtggtggtg ctggccggtc ggcgcggcag
     9601 cgtgcaggtg gacgcggccc ggtcaccctt cgtggtgacc gcgccgccgg cggacagcaa
     9661 gatgcagaag gccttcctgc tgctggagag cggcctgggc tttctcggca gcaacccgcg
     9721 cgagacgccg cggccggtgc tgtccacgcg gagcgtgggc cgggcgccgg tgatcctgag
     9781 cccgcgcgcg gggccggtca tgggtgacgc gctcaccttc gagtggcagg gctaccagtt
     9841 cgggcgctac acgctgcgcg tggtcgcggg ctccagcgtc gtgctggagc gctccggtct
     9901 cacgggcagc cggctcgtct atccggccga cgcgcccgcg ctggtccccg ggaccgccta
     9961 cacgctgcag ctcttctccg ggcagagcgt ggtggatgag atccgcttcg aggtcgcgga
    10021 ccggcagcgc gccgacgacc tgcgccggga tctccgcgag gtggaccagg cgctcggccc
    10081 ggacgtgccc gcgagctcgc gcgtggtgat gcaggcgggc gtgctggcga gccgcgggtt
    10141 cctccacgac gcgcgccgcc tggtggtggc cgctctgccc gccgacgccg atcagcctag
    10201 cctgcacgcg ctgctgggtg acctctacgc ccgcaccggc ctgccgcggg aggccgacga
    10261 agcctacgag caggcccgcg ccctcgcctc gccccccagc cgctagcttc gcgccgcgcg
    10321 cgccgcggcc gatcatcgca ccacggcatg tctagagcgg ggtgcgtgga gggggtggca
    10381 ggccctcccc gggacacgct gacccgctgg cccgcggggg ctctctcacg agccacgaga
    10441 tgggagcgtt gcgcttcggc ggcgcgcaga agacacggtc ccggggaggg cctgccaccc
    10501 cctccacgca caaccagtcc cacgcggtac gcccagcctg ctcggccccg ccagtcgatc
    10561 acacgcagcg attccacgc
//
LOCUS       DASUFA010000423         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1702877, whole genome shotgun sequence.
ACCESSION   DASUFA010000423 DASUFA010000000
VERSION     DASUFA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702877"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFX87_15305"
     CDS             <1..612
                     /locus_tag="VFX87_15305"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX4996350.1"
                     /translation="LAATEAGVDMTVTGDFTLATVMSRIKELLGDAPPAAPARRPEPP
                     VAAKGPAKMPARPPAAPVPPAKSVADPARSSFQGSLGVMDLTELTQVISLGVKTGELL
                     LSLSGGEGSILFEAGRVIHAEFAGKTGEPAFAAMVGASQREGEARFCFNRGERETLAG
                     RPRTISRSVEQLLLSVAQAIDEGTQGADGRREAAAARGPRRDG"
     gene            616..1497
                     /locus_tag="VFX87_15310"
     CDS             616..1497
                     /locus_tag="VFX87_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4996351.1"
                     /translation="MPKVLVVDDSLSVRKVVQRALESKRIEVLSAASGSEAMEQIARE
                     APDLIVCDVIMPDMDGYQICDSVKKHPTLGHTPVLLISGIVNSTVLERAAKVRSDDVM
                     RKPFAADELLHRIESLLPAAGRPAPAAAPAPAAEAAPVAPGVVPLAAPERRAPQPERA
                     APVAEPIAVAAPAPVPGPDLKALLAGVAELAGVSLTALIDREGALMEVAGGLLPEAEL
                     AGALAACLVESTDGVGRALTQGRLQSLILEFDAGVVLLNAVGAGAMLAVVLGDPAVLG
                     KVRYHVKKALPDLVRAV"
     gene            1509..>2959
                     /locus_tag="VFX87_15315"
     CDS             1509..>2959
                     /locus_tag="VFX87_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996352.1"
                     /translation="MRTSYHSVSSAQRLSSWLMALLGLAAYVGLIYVLTLVPLQFEVP
                     LPKWAVASIPPVTYGLLVWLWVRRPSVVRWLVGTAVLSGLHVVLSMSREPLSALLDPA
                     LAGRALPWMLPPPLPELIGVMLLLVPLRDVLRAPARLPRERSSVSTRPSASLRARAAT
                     LPRVQIAPPLEGSGSLSEALLSRSEPAPEPVVTPPPVMPVGPPPPAVPVPDEEPRRRP
                     AAPVVKRRREAQPARAPGRSEVVLRIALDRVMDQFPPGTFLAPEDEVAASLSDPGFLR
                     ISGELVVTQLSEGVARVAWSDIVDQFPAHLVGLSKAEITEHLGDGLRLPLDEVISQLP
                     QELFVADTPEVEIHGLHRIPVPFHPVEESDTVGPEITPAPEIRPTPPVEAPDVPTPVS
                     PAVPSWMAPPPVEHPEPVAIEPPAVPDPIEPLAPVVREPVQGREPAPVDLSTAAPETD
                     EPTVRISWGRVAAELPAEAFNVPLDQVAERMRQP"
BASE COUNT          364 a         1094 c         1078 g          423 t
ORIGIN      
        1 ctggccgcca ccgaggccgg cgtggacatg acggtcaccg gcgacttcac cctggccacc
       61 gtgatgtcgc gcatcaagga gctgctcggc gacgcgccgc ccgccgcccc cgcgcgccgc
      121 ccggagccgc ccgtggccgc caaggggccc gcgaagatgc cggcgcggcc gccggccgcc
      181 ccggtgcccc ccgccaagag cgtggccgat cccgcacgca gcagcttcca gggctcgctc
      241 ggggtcatgg acctgaccga gctcacccag gtcatctcgc tcggagtcaa gaccggcgag
      301 ctcctgctgt cgctgtcggg cggcgagggg agcatcctct tcgaggccgg ccgggtgatc
      361 cacgccgagt tcgcggggaa gaccggagag cccgccttcg ccgcgatggt gggtgcctcc
      421 cagcgggaag gcgaggcgcg cttctgcttc aaccgcggcg agcgcgagac cctggccgga
      481 cggccgcgca ccatctcgcg cagcgtggag cagctgctcc tgagcgtggc gcaggcgatc
      541 gacgagggaa cccagggagc cgacgggcgc cgggaggctg ccgccgcccg tggaccgcgg
      601 cgggacggat agctcatgcc caaggtgctg gtcgtagacg acagtctcag cgttcggaaa
      661 gtcgtgcagc gggcgctgga gtcgaagcgc atcgaggtgc tgtcggccgc ctcgggaagc
      721 gaggcgatgg agcagatcgc gcgcgaggcg ccggacctca tcgtctgcga cgtgatcatg
      781 ccggacatgg acggctacca gatctgcgac tccgtcaaga agcatccgac gctcgggcac
      841 acgccggtgc tgctgatctc gggcatcgtc aacagcaccg tgctcgagcg cgccgccaag
      901 gtccgctcgg acgacgtgat gcggaagccc ttcgcggccg atgagctgct ccatcgcatc
      961 gagagcctgc tgcccgcggc gggtcgtccc gcgccggccg ccgcgcccgc gccggctgcc
     1021 gaggcggccc cggtcgcgcc gggcgtcgtc ccgctcgcgg cgcccgagcg ccgcgcgccc
     1081 cagcccgagc gggcggcgcc tgtcgcggag ccgatcgcgg tcgccgcgcc ggcgcccgtg
     1141 ccgggtccgg atctcaaggc gctgctcgcc ggggtggccg agctggccgg agtctccctg
     1201 accgcgctga tcgatcgcga gggagccctg atggaagtcg cgggcgggct ccttcccgaa
     1261 gccgagctcg cgggcgccct ggccgcctgt ctcgtcgagt cgaccgacgg ggtgggccgg
     1321 gccctgaccc aggggcgtct ccagagcttg atcctcgaat tcgatgcggg tgtcgtgctg
     1381 ctgaatgccg tcggcgcggg cgcgatgctc gcggttgtgc ttggcgatcc ggcggtgctc
     1441 ggcaaggtgc ggtaccacgt gaagaaagcg ctcccggacc tggtccgggc cgtgtgaggc
     1501 cttcctccat gagaacgtcc taccattccg tgtccagcgc ccagcggctc tcgtcgtggc
     1561 tgatggccct gctggggctg gccgcgtatg tcggcctcat ctacgtcctg accctcgtgc
     1621 cgcttcagtt cgaggtgccg ctgccgaagt gggccgtcgc gagcatcccg ccggtgacgt
     1681 acggcctgct ggtgtggcta tgggttcgcc gtccatccgt cgtgcgatgg ctcgtgggca
     1741 ccgcggttct gagcggcctc cacgtcgtgc tgagcatgtc gcgggagcct ctcagcgcgc
     1801 tgctcgatcc ggccctcgcg gggcgcgcgc tgccgtggat gctgccgccg cccttgccgg
     1861 agttgatcgg cgtgatgctg ctgctggtgc cgctccgtga cgtgctgcgc gcgcccgccc
     1921 gtcttccccg ggagcggagc tccgtcagca cccgcccgtc ggcgagcctt cgggcccgtg
     1981 ccgccacgct cccgcgcgtg cagatcgcgc ccccgctcga ggggagcggc tccttgagcg
     2041 aagcgctgct ctcgcgcagc gagccggctc ccgagcccgt cgtgaccccc cctccggtga
     2101 tgccggtggg gccgccgcca ccggccgtgc cggtgccgga cgaggagccc cgtcggcgcc
     2161 ccgccgcccc ggtcgtgaag cgccggcgcg aagcccagcc ggcccgcgcg cccggccgat
     2221 cggaagtggt tctgcggatc gcgctcgacc gcgtcatgga ccagttcccc cccggcacct
     2281 tcctcgcgcc ggaggacgag gtggcggcga gcctcagcga ccccgggttc ctccggatct
     2341 cgggcgagct cgtggtcacg cagctctcgg aaggtgtcgc gcgggtcgcg tggagcgaca
     2401 tcgtcgatca gttcccggct cacctggtgg gactgagcaa ggccgagatc accgagcacc
     2461 tgggagacgg cctgcggctc cccctcgacg aggtgatcag ccagctcccg caggagttgt
     2521 tcgtcgcgga cacgccggag gtcgagatcc acgggctcca ccggatcccg gtgccgttcc
     2581 atccggtcga ggagtccgac acggtggggc cggagatcac gccggcgccc gagatcaggc
     2641 ccactccgcc cgtcgaagca ccggacgtgc ccacgccggt ctcacctgcc gtgccatcgt
     2701 ggatggcgcc gccgcccgtg gagcatccgg aaccggtcgc gatcgagccg cccgccgtgc
     2761 ccgatccgat cgagccgctg gcgcccgtcg tgcgcgagcc ggtgcagggg cgcgagccgg
     2821 ccccggtcga tctctcgacc gccgctcccg agaccgacga gcccaccgtg cgcatctcct
     2881 ggggccgcgt ggcggcggag ctgcccgcgg aggcgttcaa cgtcccgctg gaccaggtgg
     2941 ccgagcggat gcgacagcc
//
LOCUS       DASUFA010000424         7849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_195673, whole genome shotgun sequence.
ACCESSION   DASUFA010000424 DASUFA010000000
VERSION     DASUFA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195673"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFX87_15320"
     CDS             <1..1293
                     /locus_tag="VFX87_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4996353.1"
                     /translation="PERLTVDMVRLIHLTRPGDNARSLEGMLTSCATQPVFETPRHSW
                     GPTARIEFAWEPIVPGAEYRVSVTAVSCSQPDRQREVLREMTGATAVTLSLPPSVGDE
                     QYFFWIAAWRDGRLVGDLYTHDAGAHSWNYRFQVRNASLSRWVYVAAAGLLLLLVIGA
                     GRLLPRAAPTRRRRLARRALALVVVGALAGAAYYIVQDRVQEGERRRIEEERAQSEAE
                     GQARQRELITAFVSAAPRPAWWASVQTPYRVDNVGDLLAAWQGFPRGDDGLGERQFFK
                     AAYRGIVDHPDDEHVVATAIDLLHWVVDDYPHRLPLAQFGYDRYFQHRQRVDDCANCM
                     VGDRTQGLTQNLSRLLMAAGRYDEAIAALRRLIDERGADVSPYRLAETWNRLAWAHWE
                     KGERERAREVIREAIAKYGGTVRGDDLRRTLATFERAP"
     gene            complement(1330..1977)
                     /locus_tag="VFX87_15325"
     CDS             complement(1330..1977)
                     /locus_tag="VFX87_15325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4996354.1"
                     /translation="MLREFALEPIGPVSIEETSAQNLLKLSTHTYWRRVWPAGLALAA
                     YITDRFGADGLRGRQVLDLGCGVGLIGIVCGRLGASVTFLDREAEALAAVRRNCRRNG
                     LGSAQTIGGDWNHGGDRLAPATYDLVVGGDVVYDDAEWPALGTGLMRTLRPEGQALLA
                     DPGWVPDAKLRAAFRRSGFSVTRDRRTVSWPPWRPTHRQDKIINIYTLRRAPHAP"
     gene            2084..2974
                     /locus_tag="VFX87_15330"
     CDS             2084..2974
                     /locus_tag="VFX87_15330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4996355.1"
                     /translation="MALSVGFIGVGNMGNPMAGNVLKAGFSMTVFDMNPKAMENLIQA
                     GAKRAGSAKEVVDASDVVLTSLPASPDVEAMYLDKGGLVERAKPGTILIDLSSVLPKT
                     PRMIEPVAKARGVHFLEAPVSGGVTGARAATLAIMVGGDAAAMEKARPVLRAMGPNIF
                     HVGPAGAGNTIKAINNMMACVNSAAMMEGFILGVKAGLDPMTIYEVVKASSGGSKAIE
                     RIPRALIPRDFEPGFKVALMNKDLETFHTIAKELHVPVTFSNLAQHYQQAALAAGLGE
                     QDTSVVFTIIERLAGMKAPK"
     gene            2971..3351
                     /locus_tag="VFX87_15335"
     CDS             2971..3351
                     /locus_tag="VFX87_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4996356.1"
                     /translation="MTARRQPAKAAKPARNSKNPYGYSFWEWVAQEDPEYFRARQPLS
                     ALSIGEGKELSVKYREMVIIGILAFRGRKEGVVAHMRRAIEHGATKRELLEAIQSAAV
                     PGGGPTFSTGAQALMELDRQGAFR"
     gene            complement(3371..4372)
                     /locus_tag="VFX87_15340"
     CDS             complement(3371..4372)
                     /locus_tag="VFX87_15340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4996357.1"
                     /translation="MRSIDIHAHVVPRAVWQAAEAGREWYGFRHQGGDGLGTFLGGNG
                     KRTGFTSPKVRFTPEERLADMDAQGVDVQVLSIHTPFFGYHLDAAQGTALAREVNDEI
                     ATMTRQWPTRFAGLATLPMQDTKAAIQELERAVTGLGLKGAELDTIVNGENWDAPRFL
                     PFFKAAEAMGAVLFYHPQPQHNFLTERTTRYGLFNSLGVILEDAIVVAILILGGVLEA
                     CPALKVCIAHGGGPACYAMGRLDRGWQGNADARQSIPRAPSSYQKQLYYDTVVGSEAA
                     LRFMIDQVGIDRVVLGSDWPFVPWHPSPVTWVEGLRSLTREEKDRILWRNLETLLAL"
     gene            complement(4398..6704)
                     /locus_tag="VFX87_15345"
     CDS             complement(4398..6704)
                     /locus_tag="VFX87_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX4996358.1"
                     /translation="MSERQGDGWIGRPLRRREDHRLLTGAGAFVDDMTPPGCLHVALL
                     RSPHAHARVVRLDVEAARSAPGTRLVVTGKDVAHLGPMPVNRLVPDMRVPPHPILAES
                     QVHAVGMPVAAVVADDVYAAYDALDLISVDYEPLPGLAEPEAALASGAPLLFSGIDGN
                     RVLTRIIREGDAHRAFAAAAHVVPLRVAQNRISAVAMEPRSVLATFDRTSSELTMWVS
                     CQAPFRIRGEVARLLDLPESRVRVIAPDVGGGFGVKTGPYREDVLLAWLTRRLGRPVK
                     WVATRREDQVTTNQARGSVCEGALALDAQGRITGLRGTITAPLGAQLMNAAAGSPWNH
                     ARCLPGAYVVPSCDITVNGALTTTTPVSAYRGAGRPEACFFIERLMDTAARTIGLDPA
                     EIRRRNLIPANRFPFKTITGQVYDSGDYPRALDNALRAADYDGLRRRQAERRAKGEIV
                     GVGLASYVEPCALGWESGSIRVERSGKVTAITGSSAHGQGHETTFAQVVADHLGVTPD
                     EVTVMHGDTRSGPEGFGTFGSRSVALGGGALVRTSVEVRDKGRRIAARLLEAAPEDIV
                     PVRGGFQVAGVPGKRVAWKEVATAAYAGGQALPPGDTPGLEATTYFQPDGEVWSSGAV
                     VCGVRIERETGELHVEKLVWVDDAGTIINPLLAEGQLLGALAQGMGQALMEAIVYDAD
                     GQCLTTTLMDYAIPRATDMPAVLIEKMHTASPRNPLGAKGLGEAGCIAMPPAMVNAAV
                     DALAPLGIGHLDMPLTPERLHAALTERR"
     gene            complement(6701..7483)
                     /locus_tag="VFX87_15350"
     CDS             complement(6701..7483)
                     /locus_tag="VFX87_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4996359.1"
                     /translation="MKARTIETKKGARCRVLEGGSGMPVVFFHGAGGLLADNPFLDQL
                     AARYHVFAPELPGYGESTGEELLEDMLDFTLHGWDVVSALGLTRPHLVGHSMGGMIAA
                     EMAAIAPGDLDRLVLVAAAGLWLDEHPIPDIFALLPGQLIELLFQDPVRGQTLLTGGA
                     DFSDMEVFKEFYLGQQRRLAMAGKILFPIPNRRVSKRLYRVAAKTLVLWGGADKLIVP
                     AYAERWGRLIPGATVEIVPDAAHMLPYEQPAAFVGAVTRFLG"
     gene            complement(7480..>7849)
                     /locus_tag="VFX87_15355"
     CDS             complement(7480..>7849)
                     /locus_tag="VFX87_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996360.1"
                     /translation="YGALSQLRLEAQKLMANLTWKQLVDERFIIAGSPETVRQQLEEC
                     IKGLRIGHLFCLLHTGNMPDWKTRHSTKLFAEKVMPHLRNLWPSFQHDDRWWIHPMED
                     RLHPEEMQPGAEKPGQVWPR"
BASE COUNT         1121 a         2759 c         2862 g         1107 t
ORIGIN      
        1 ccggagcgcc tcaccgtgga catggtccgg ctcatccacc tgacgcgccc gggcgacaat
       61 gcccgctcgc tcgaaggcat gctcacgagc tgcgcgaccc agcccgtctt cgagacgccg
      121 cgccactcct gggggccgac ggcgcgcatc gagtttgcgt gggagccgat cgtccccggc
      181 gccgagtacc gggtgagcgt gaccgcggtg tcgtgctccc agccggaccg gcagcgggag
      241 gtcctgcgtg agatgacggg cgcgaccgcg gtgaccctga gcctgccgcc gagcgtgggc
      301 gacgagcagt acttcttctg gatcgcggcc tggcgcgacg gccggctcgt cggggatctc
      361 tacacccacg acgccggcgc gcactcgtgg aactaccgct tccaggtccg caacgcgagc
      421 ctgtcgcgat gggtctacgt ggccgccgcc ggcctcctgc tgctcctcgt catcggcgcg
      481 gggcggctcc ttcccagggc cgcgcccacg cgccggcggc ggctcgcgcg ccgggcgctc
      541 gcgctggtcg tcgtcggcgc gctcgcggga gccgcctact acatcgtcca ggaccgggtc
      601 caggaaggtg agcggcgccg catcgaggag gagcgcgcgc agagcgaggc cgagggccag
      661 gcgcggcagc gcgagctcat caccgcgttc gtctcggccg cgccgcggcc ggcctggtgg
      721 gcctcggtgc agaccccgta ccgggtggac aacgtgggcg acctgctggc ggcctggcag
      781 ggcttcccgc gcggggacga tggtctgggc gagcggcaat tcttcaaggc cgcctaccgc
      841 gggatcgtgg atcatccgga cgacgagcac gtggtggcga ccgccatcga cctgctccac
      901 tgggtcgtgg acgactatcc gcaccgcctg cccctcgcgc agttcggcta cgaccgctat
      961 ttccaacacc ggcagcgcgt ggacgactgc gccaactgca tggtggggga ccggacccag
     1021 gggctgaccc agaacctgag ccggctcttg atggcagccg gccgctacga cgaggccatc
     1081 gcggcgttgc ggcggctgat cgatgagcgc ggggccgacg tcagccccta ccggctggcc
     1141 gagacctgga atcgactcgc gtgggcgcac tgggagaagg gcgagcggga gcgggcccgg
     1201 gaggtcatcc gggaggcgat cgcgaagtac ggcgggacgg tgcgcggcga tgacctgcgc
     1261 cgcacgctgg ccaccttcga gcgcgcgccc tgacctgcgc gtcggccgcg ggcgggagcg
     1321 aggtgaggac tacggagcgt gcggggcccg gcggagcgtg tagatgttga tgatcttgtc
     1381 ctgccggtgg gtcggccgcc aggggggcca gctcaccgtc cggcgatcgc gggtgacgct
     1441 gaagccactt cttcggaagg ccgcccgcag cttcgcgtcg gggacccagc cggggtcggc
     1501 cagcagcgcc tggccctccg gccgcagcgt tcgcatgagc ccggtgccga gggccggcca
     1561 ctcggcgtcg tcgtagacca cgtcgccgcc caccaccagg tcataggtgg ccggcgccag
     1621 gcggtccccc ccgtggttcc agtcgccgcc tatggtctgg gcagaaccca ggccattgcg
     1681 ccggcaattg cgccggacgg cggcgagagc ctcggcctcg cggtccagga aggtgacgct
     1741 cgcgcccagg cgtccgcaga cgatgccgat gaggccgacc ccgcagccca ggtccaggac
     1801 ctggcggccc cggaggccgt cggccccgaa gcggtcggtg atgtaggcgg cgagcgccag
     1861 gcccgccggc cagacccgcc gccagtaggt gtgggtggag agcttgagga gattctgtgc
     1921 cgacgtctcc tcgatggaga cgggcccgat cggctccagc gcgaactctc gtagcactag
     1981 gccggagtct aacgcacgtt gatccgtccg tcgcgcaggt ttatgctcgg cgcgtcccgg
     2041 tcggttctcg cagtgacccc agtcccgcca aaggaggcgc gcaatggctc tctccgtagg
     2101 gttcatcggt gtggggaaca tgggcaaccc gatggccggc aacgtgctga aggccggctt
     2161 ctcgatgacg gtgttcgaca tgaaccccaa ggccatggag aacctgatcc aggcgggggc
     2221 caaacgggcc ggctccgcca aggaggtggt ggacgcctcc gacgtcgtgc tgacctcgtt
     2281 gcccgcctcc ccggacgtgg aggccatgta cctcgacaag ggtggcctgg tggagcgcgc
     2341 caagccgggc accatcctga tcgacctgag cagcgtgctg cccaagaccc ctcggatgat
     2401 cgagccggtc gcgaaggctc gcggcgtgca cttcctggag gcgccggtca gcggcggagt
     2461 caccggagcc cgcgcagcca cgctcgccat catggtcggc ggcgacgcgg cggcgatgga
     2521 gaaggcgcgg ccggtgctgc gcgccatggg cccgaacatc ttccacgtgg ggccggccgg
     2581 cgcgggtaac accatcaagg ccatcaacaa catgatggcg tgcgtgaaca gcgccgcgat
     2641 gatggaaggc ttcatcctgg gcgtgaaggc ggggctcgac ccgatgacga tctacgaagt
     2701 ggtgaaggcg agcagcggcg gcagcaaggc catcgagcgc atcccgcgcg ccctgatccc
     2761 gcgcgacttc gagccgggct tcaaggtggc gctgatgaac aaggacctcg agaccttcca
     2821 caccatcgcc aaggagctcc acgtgccggt gacgttctcg aacctggccc agcactatca
     2881 gcaggcggcg ctggccgcgg ggctcgggga gcaggacacc agcgtggtct tcacgatcat
     2941 cgagcggctg gccggcatga aggcgccgaa gtgaccgcgc gccgtcagcc cgccaaggcg
     3001 gccaagccgg cgcgcaacag caagaacccc tacggctact ccttctggga gtgggtcgcc
     3061 caggaggacc cggagtactt ccgggcgcgc cagccgctga gcgcgctctc gatcggcgag
     3121 ggcaaggagc tgtcggtgaa gtaccgcgag atggtgatca tcggcatcct cgccttccgc
     3181 ggccggaagg aaggcgtggt cgcgcacatg cgccgggcca tcgagcacgg cgccaccaag
     3241 cgtgagctgc tggaggcgat ccagtccgcc gcagtgccgg gcggcggccc cacgttcagc
     3301 accggcgcgc aggccttgat ggagctcgat cgccagggag cgttccgcta gccagggcgc
     3361 gctggaggac tcagagagcg agcagcgtct cgaggttgcg ccagaggatg cggtccttct
     3421 cctcccgggt gaggctccgg aggccttcca cccacgtgac cggcgagggg tgccacggca
     3481 cgaacggcca gtcgctgccg agcacgacgc ggtcgatgcc gacctggtcg atcatgaagc
     3541 ggagcgcggc ctcgctgccc accaccgtgt cgtagtagag ctgcttctgg tagctgctcg
     3601 gggcccgcgg gatgctctgg cgcgcgtcgg cattgccctg ccagccgcgg tcgaggcgcc
     3661 ccatcgcgta gcaggccggc ccgcccccgt gggcgatgca caccttgaga gccgggcacg
     3721 cctccagcac cccgcccagg atcaggatcg cgaccacgat ggcgtcctcg aggatgacgc
     3781 ccaggctgtt gaagagcccg tagcgcgtgg tgcgctcggt caggaaattg tgctggggct
     3841 gcggatggta gaagagcacg gcgcccatgg cctcggcggc cttgaagaag ggcaggaagc
     3901 gcggcgcgtc ccagttctcg ccgttcacga tcgtgtcgag ctccgcgccc ttgagcccga
     3961 ggccggtcac cgcgcgctcc agctcctgga tcgccgcctt cgtgtcctgc atgggcaggg
     4021 tggcgaggcc ggcgaagcgg gtgggccact gccgcgtcat cgtcgcgatc tcgtcgttga
     4081 cctcgcgcgc gagcgccgtg ccctgcgccg cgtcgaggtg gtagccgaag aacggggtgt
     4141 ggatggagag cacctgcacg tccacgccct gcgcgtccat gtcggcgagg cgctcctccg
     4201 gcgtgaagcg caccttgggc gaggtgaagc cggtgcgctt gccgttgccg cccaggaagg
     4261 tgccgaggcc gtcgccgccc tggtggcgga agccgtacca ctcgcgtccg gcctccgcgg
     4321 cctgccagac cgcccggggt accacgtgcg cgtggatgtc gatggatcgc atgggtcgtc
     4381 cttgcgccgg cggttcgcta gcgccgctcc gtgagcgcgg cgtgcaggcg ctcgggggtg
     4441 agcggcatgt cgaggtggcc gatgccgagc ggcgcgagcg cgtccaccgc cgcgttcacc
     4501 atcgcgggcg gcatcgcgat gcagccggcc tcgcccaggc ccttggcccc gagcgggttg
     4561 cgcggcgacg cggtgtgcat cttctcgatg agcacggccg gcatgtcggt ggcgcgcggg
     4621 atcgcgtagt ccatcagcgt cgtggtgagg cactggccgt cggcgtcgta gacgatggcc
     4681 tccatcaggg cctggcccat gccctgggcc agcgcgccca ggagctggcc ctcggccagg
     4741 aggggattga tgatcgtccc ggcgtcgtcc acccagacga gcttctcgac gtgcagctcg
     4801 ccggtctcgc gctcgatgcg gaccccgcag accaccgcgc cggagctcca gacttcccca
     4861 tccggctgaa agtaggtggt ggcctcgagc cccggggtgt cccccggcgg cagggcctgg
     4921 ccgcccgcgt aggcggcggt ggcgacctcc ttccacgcca cgcgcttgcc cggcacgccc
     4981 gccacctgga agccgccgcg caccggcacg atgtcctcgg gcgcggcctc gagcagccgg
     5041 gccgcgatgc gccggccctt gtcgcgcacc tcgaccgagg tgcgcacgag cgcaccgccg
     5101 ccgagcgcga cgctgcggct gccgaacgtg ccgaagccct cggggccgga gcgggtgtcg
     5161 ccgtgcatca ccgtgacctc gtccggcgtg acgcccaggt gatcggccac cacctgcgcg
     5221 aacgtggtct cgtgcccctg gccgtgggcg ctcgagccgg tgatggcggt gaccttccct
     5281 gatcgctcca cgcgaatcga cccgctctcc catccgagcg cgcacggctc cacgtacgag
     5341 gcgagcccca cgccgacgat ctcgcccttc gcgcgccgtt cggcttgccg ccgcctcagc
     5401 ccgtcgtagt cggcggctct gagcgcgttg tccagcgccc gtggatagtc gcccgagtcg
     5461 tagacctggc cggtgatggt cttgaacggg aagcggttcg cgggaatgag gtttcgccgc
     5521 cggatctccg ccgggtcgag cccgatcgtc cgggccgcgg tgtccatcag ccgctcgatg
     5581 aagaagcacg cctccggccg gccggcgccc cgataggccg agaccggcgt ggtggtggtg
     5641 agcgcgccgt tgaccgtgat gtcgcagctc gggaccacgt aggccccggg caggcagcgc
     5701 gcgtggttcc acggcgagcc cgcggccgcg ttcatgagct gagcgccgag tggggcggtg
     5761 atcgtcccgc ggagtccggt gatacgcccc tgtgcatcga gcgcgagcgc accctcgcag
     5821 accgagccac gcgcctggtt ggtggtgacc tggtcctcgc ggcgggtggc cacccacttg
     5881 accggccggc cgagccgccg cgtcagccac gccagcagca cgtcctcgcg gtagggcccg
     5941 gtcttcaccc cgaacccgcc gcccacgtcg ggggcgatga cgcgcacccg gctctccggc
     6001 agatcgagca ggcgcgcgac ctcgccgcgg atgcggaacg gcgcctggca ggacacccac
     6061 atggtcagct cggaggacgt gcggtcgaac gtggccagga ccgagcgcgg ctccatcgcc
     6121 accgcggaga tgcggttctg cgccacgcgc agcggcacga cgtgggccgc cgccgcgaag
     6181 gcgcgatgag cgtcaccttc ccgaatgatc cgcgtgagca cgcggttgcc gtcgatgccg
     6241 gagaagagca gcggggcgcc gctcgccagg gcggcctcgg gctcggccag gccgggcagc
     6301 ggctcgtagt ccacgctgat gagatcgagc gcgtcgtagg ccgcgtagac atcgtcggcc
     6361 accaccgcgg ccaccggcat gccgaccgcg tggacctggc tctcggccag gatggggtgc
     6421 ggcggcacgc gcatgtcggg caccagccgg ttcaccggca tgggcccgag atgcgccacg
     6481 tccttgccgg tgaccaccag ccgcgtgccc ggcgcgctcc gcgcggcctc gacgtcgagg
     6541 cggacgaccc tggcgtgggc gtgcggggag cggagcaggg ccacgtgcag gcaccccggc
     6601 ggcgtcatgt cgtccacgaa ggcgcccgcc ccggtcagga ggcggtggtc ctcgcgccgc
     6661 ctgaggggcc ggccgatcca gccgtcgccc tggcgctcgc tcatccgagg aaccgggtga
     6721 ccgcgccgac gaaggcggcc ggctgctcgt acggcagcat gtgcgcggca tccgggacga
     6781 tctccacggt cgcgcccggg atcagccggc cccaccgctc ggcataggcc gggacgatga
     6841 gcttgtccgc tccgccccag aggaccaggg tcttcgcggc cacccggtag aggcgcttcg
     6901 acacccggcg gttcgggatc gggaagagga tcttgcccgc catcgccagc cgccgctgct
     6961 ggcccaggta gaactccttg aagacctcca tgtccgagaa gtccgcgccg ccggtcagca
     7021 gcgtctggcc ccgcaccgga tcctggaaga gcagctcgat gagctggccg gggagcaggg
     7081 cgaagatgtc gggaatcggg tgctcgtcga gccagagccc cgccgccgcg accagcacca
     7141 gccggtcgag atcgccgggc gcgatcgcgg ccatctcggc ggcgatcatg ccgcccatcg
     7201 agtggccgac caggtgcggc ctcgtcagcc cgagcgccga gaccacgtcc cagccgtgga
     7261 gcgtgaagtc gagcatgtcc tccagcagct cctcgccggt ggactcgccg tagcccggca
     7321 gctcgggggc gaagacgtga tagcgcgccg cgagctggtc gaggaacggg ttgtcggcca
     7381 gcaggccacc cgcgccgtgg aagaacacga ccggcatgcc gctgcccccc tcgagcacgc
     7441 ggcagcgcgc gcccttcttc gtctcgatcg tgcgcgcctt catcttggcc acacctgccc
     7501 gggcttctcg gccccgggct gcatctcctc cggatgcagg cggtcctcca tcgggtggat
     7561 ccaccagcgg tcgtcgtgct ggaatgaagg ccacaggttg cggagatgcg gcatgacctt
     7621 ctcggcgaag agcttggtgg agtggcgcgt cttccagtcc ggcatgttgc cggtgtggag
     7681 caggcagaag aggtggccga tccgcaggcc cttgatgcac tcctcgagct gctggcgcac
     7741 cgtctccggg ctgcccgcga tgatgaagcg ctcgtccacg agctgcttcc aggtgaggtt
     7801 ggccatcagc ttctgcgcct cgaggcggag ctgcgagagc gcgccgtac
//
LOCUS       DASUFA010000425         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1253161, whole genome shotgun sequence.
ACCESSION   DASUFA010000425 DASUFA010000000
VERSION     DASUFA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253161"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFX87_15360"
     CDS             complement(<1..535)
                     /locus_tag="VFX87_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996361.1"
                     /translation="MKRRAFVKGAGAVAVVAAGGLVWRAYDQGVFSAGAGAAYEPWTN
                     WRSDAAPGPLGLVRAGILAANPHNTQPWLFQVGATRIELFADIRRNLGAFDPYLREMH
                     IGLGCALENMMRTARVLGYDVAVTLHPGPLAAAPVSLRPQPVATLALSPARPRPDRLV
                     EAIPRRHTDRGAYAAGHP"
     gene            648..1862
                     /locus_tag="VFX87_15365"
     CDS             648..1862
                     /locus_tag="VFX87_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996362.1"
                     /translation="MDLNYTAEDVAFRKQVRAWLEQNMPRHIKDLDERKTWHRKLYEA
                     GYLGMGWPKEYGGQGARPMEQAIVADEMARAGAPAPTNGLGVGIVGPTIVVHGTEAQK
                     RRYLRKILTAEEMWCQLYSEPNAGSDLAALRTAAEDKGDHFQINGQKIWTSGGAIADW
                     GLLLARTDPAVAKHKGISCFLINMRQPGVDVRPLKQITGSSEFCEVFMTNARVEKENQ
                     IGRLGEGWGIAQTTLGYERGGRALARITSYAGQYNQLMNAARRLKRNGRPILDDAVTR
                     QKLGRIWADLEVERYGALRVLTMLERGEHPGAGGSLTKLSYSEFEKRFMELAEEILGP
                     YGQLTDGVPPEFRLEVDTAVGDHGTWAYAFLWSRAGTIYAGSSEIQKNVIGERILGLP
                     KEARADRVGGTR"
     gene            1859..2980
                     /locus_tag="VFX87_15370"
     CDS             1859..2980
                     /locus_tag="VFX87_15370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996363.1"
                     /translation="MNFSFSEDQTLLRNSVRAALDEQCKPAHVRAMMEDGGGYSEALW
                     GEMAKLGWLGLPFAEEQGGAGLGMVELALVLEEMGRAAYPGPYFASVVLAGLGIQAGG
                     SAAQKEKWLPAIASGQARASAALLEDSLDWDPAATTTTATRTGAGFRLDGVKRFVPWA
                     HVADVVLVPARAPEGVSLFLVDPRAAGITLTPMVGIDLATRWSEMRLDNVAVPADALM
                     GPAGGAGPVLEGLLRRAAVGASAEMLGAARRCLDMSVGYAKVREQFGQLIGSFQAIRH
                     RCAEMLLEVEKAHAAVYYAAWALGAGAEDATMAASVCKSFVSEAARQVCGDAIQVHGG
                     IGFTWEYDLHLYFKRAKALEPLYGDAAYHRELLIRGQTA"
     gene            2980..4197
                     /locus_tag="VFX87_15375"
     CDS             2980..4197
                     /locus_tag="VFX87_15375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4996364.1"
                     /translation="MATQEEPPHPTPLPPRGGTRGEGGALEGVRVVDLTSYIAGSYGA
                     MMLADLGADVIKVEAIEGDSFRELPGFYGWNRGKRSIAVNVKDPDGRAIVHRLAREAD
                     VVMENMRPGVVERLGVDYETLRALTPRLIYATVTAFGSDGPYQERPGFDPLLQAMGGV
                     MTLQGFGGAPQYLRVAVTDYYCAALNCQMVLAALFARERTGKGQRVQTSLLQAVLALQ
                     SGNVVAYPGNEVVYRSTPTYRLYQGRDGEWFFLAVGNQSFWVKLCKAVGREDLAQDPR
                     FGSWFSRRDNADALMPLLEEAFGSKPAAEWVRILTAHDIPAAVTQSLREFMRDPAVLH
                     HKMIVEYDHPELGPLSLMGQPLRFSETQAPDAGPPPLLGQHTAEVLREAGFADPEIAE
                     LRRRGVVAGKDIP"
     gene            4194..5006
                     /locus_tag="VFX87_15380"
     CDS             4194..5006
                     /locus_tag="VFX87_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4996365.1"
                     /translation="MTDAKIDPARTKYEAIRYEQADGVATITLNRPDVHNAMNQAMRR
                     ELLEVFTRLRTDDTVRAVVVTGGGDKAFSAGADIREFLEPPVPTQFREARRRVDFRRE
                     MERCPQPIIAAIRGYAFGGGLELALACDIRVAAEDAQLGLTEINLAIIPGGGGTQRLP
                     RLVGRGKALEMILTGMRIPAAEALRIGLVERVVPLAEHPSAAQALARTIADKAPVALR
                     YAKEAVVGGIGLPLEDGIRLENDLATLLRTTEDRLEGAKAFVEKRKPRWTGK"
     gene            complement(5011..>5219)
                     /locus_tag="VFX87_15385"
     CDS             complement(5011..>5219)
                     /locus_tag="VFX87_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4996366.1"
                     /translation="GWTEEESRPLLDYLFRQQVRPELTCRFQWQPGSIAFWDNRCVQH
                     NAVNDYAGFKRLMHRVTLAGDTPR"
BASE COUNT          779 a         1863 c         1888 g          689 t
ORIGIN      
        1 ccgggtgccc ggccgcgtag gccccgcgat cggtgtgccg ccgcgggatg gcctccacga
       61 gccggtccgg ccgcggccgc gcgggggaga gcgcgagcgt cgccaccggc tgcgggcgga
      121 gcgacacggg ggccgccgcg agcgggcccg gatgcagggt gaccgcgacg tcgtagccca
      181 ggacgcgggc ggtccgcatc atgttctcga gcgcgcagcc gaggccgatg tgcatctcgc
      241 gcaggtaggg atcgaacgcg ccgaggttgc gccggatgtc ggcgaagagc tcgatgcgcg
      301 tcgcgcccac ctggaagagc cacggctgcg tgttgtgggg attggcggcg aggatgccgg
      361 cccgcaccag cccgagcggc ccgggcgccg cgtcgctccg ccagttcgtc cacggctcgt
      421 aggccgcccc ggccccggcc gagaagacgc cctgatcgta cgcgcgccag accagtcccc
      481 cggcggccac gaccgcgacg gctcccgctc ccttcacgaa ggcccggcgc ttcacgcgag
      541 gaaaaggtag cacggcggcc cggttgaccg tcgcgcgggg cccgtgctac aagcggggct
      601 acccccaacg gcagcgccag ccgcagaccc ggcaaggagg ccccgccatg gatctcaact
      661 acaccgccga ggacgtcgcc tttcgcaagc aggtccgcgc gtggctcgag cagaacatgc
      721 cgcggcacat caaggatctc gacgagcgca agacctggca ccgcaagctg tacgaggccg
      781 gctatctcgg catggggtgg cccaaggaat acggcggcca gggcgcgcgg ccgatggagc
      841 aggcgatcgt ggccgacgag atggcgcgcg cgggcgcgcc cgccccgacc aacgggctcg
      901 gcgtcggcat cgtcggcccc accatcgtgg tgcacggcac cgaggcccag aagcgccgct
      961 atctccggaa gatcctcacc gccgaggaga tgtggtgcca gctctactcg gagccgaacg
     1021 cgggctccga cctggccgcg ctgcgcaccg cggcggagga caagggggac cacttccaga
     1081 tcaacggcca gaagatctgg acgagcggcg gcgccatcgc cgactggggg ctgctgctcg
     1141 cgcgcaccga tcccgcggtg gccaagcaca agggcatcag ctgcttcctc atcaacatgc
     1201 gccagcccgg ggtggacgtg cgcccgctca agcagatcac cggctcctcg gaattctgcg
     1261 aggtcttcat gaccaacgcg cgggtggaga aggagaacca gatcgggcgc ctgggcgagg
     1321 gctggggcat cgcgcagacc acgctgggct acgagcgcgg gggccgcgcg ctggcccgca
     1381 tcaccagcta cgcggggcag tacaaccagc tgatgaatgc ggcgcgacgg ctcaagcgga
     1441 acgggcggcc catcctggac gacgcggtga cgcgccagaa gctcggccgc atctgggccg
     1501 acctcgaggt ggagcgctac ggcgccctgc gcgtgctgac catgctggag cgcggcgagc
     1561 accccggggc gggcggctcg ctcaccaagc tctcctactc cgagttcgag aagcggttca
     1621 tggagctggc cgaggagatc ctggggccgt acgggcagct caccgacggc gtcccgccgg
     1681 agtttcgcct ggaagtggac accgcggtgg gcgatcacgg cacctgggcc tacgcgttcc
     1741 tgtggtcgcg cgcgggcacg atctacgccg gctcctcgga gatccagaag aacgtcatcg
     1801 gcgagcgcat cctcgggctg ccgaaggaag cccgggccga tcgcgtcgga ggtactcgat
     1861 gaatttctcg ttcagcgaag accagacgct gctccgcaac tccgtccgcg ccgcgctgga
     1921 cgagcagtgc aagcccgccc acgtgcgcgc gatgatggag gacggcggcg gctacagcga
     1981 agcgctctgg ggcgagatgg ccaagctcgg ctggctcggc ctgcccttcg ccgaggagca
     2041 gggcggcgcc ggcctcggca tggtggagct ggccctcgtg ctggaggaga tgggccgcgc
     2101 cgcctatccc ggcccgtact tcgccagcgt ggtgctggcc ggcctcggga tccaggccgg
     2161 cggcagcgcg gcgcagaagg agaagtggct ccccgccatc gcctccggtc aggcgcgcgc
     2221 cagcgcggca ctgctggagg actcgctcga ctgggatccc gcggccacca ccacgacggc
     2281 cacgaggacc ggcgccggtt ttcgcctcga cggggtgaag cgcttcgtgc cgtgggccca
     2341 cgtggccgac gtggtgctcg tccccgcgcg cgcgcccgag ggcgtctcac tcttcctggt
     2401 ggacccgcgc gccgccggca tcacgctgac gccgatggtc ggcatcgacc tcgccacccg
     2461 ctggtcggag atgcgcctcg acaacgtggc ggtgcccgcg gacgcgctga tgggcccggc
     2521 cggcggcgcc ggcccggtgc tggaggggct gctccggcgc gcggcggtgg gcgcctccgc
     2581 cgagatgctg ggcgcggcgc gccgctgcct cgacatgagc gtgggctacg cgaaggtgcg
     2641 cgagcagttc ggccagctca tcggctcctt ccaggcgatc cgccaccgct gcgcggagat
     2701 gctgctggaa gtggagaagg cgcacgccgc ggtctactac gcggcctggg cgctcggcgc
     2761 gggcgccgag gacgcgacca tggccgcgtc ggtctgcaag tcgttcgtga gcgaggccgc
     2821 gcggcaggtc tgcggcgacg ccatccaggt gcacggcggc atcggcttca cctgggagta
     2881 cgacctccac ctctacttca agcgcgccaa ggcgctggag cccctgtacg gcgacgccgc
     2941 ctaccatcgc gagctcttga tcaggggtca gaccgcctga tggccaccca ggaagagccc
     3001 cctcacccca cccctctccc cccccggggg ggaacgcggg gtgagggggg cgcgctcgaa
     3061 ggcgttcgcg tggtggatct gacctcgtac atcgcggggt cgtacggggc gatgatgctg
     3121 gcggacctgg gcgccgacgt catcaaggtg gaggcgatcg aaggcgactc gttccgcgag
     3181 ctgccgggct tctacggctg gaaccgcggc aagcgatcga tcgcggtgaa cgtcaaggac
     3241 cccgacggcc gcgccatcgt ccaccgcctg gcccgcgagg ccgacgtggt catggagaac
     3301 atgcgccccg gcgtggtcga gcgcctgggc gtggactacg agacgctccg agcgctgact
     3361 ccgcggttga tctacgccac cgtgaccgcc ttcggctccg atggtcccta ccaggagcgc
     3421 cccggcttcg accccctgct gcaggcgatg ggcggcgtga tgacgctcca gggcttcggg
     3481 ggcgcgcccc agtacctccg cgtcgcggtc accgactatt attgcgcggc gctcaactgc
     3541 cagatggtgc tggccgcgct ctttgcgcgc gagcgcacgg gcaaaggcca gcgcgtgcag
     3601 acctcgctgc tgcaggcggt gctcgcgctt cagtccggca acgtggtggc ctacccgggt
     3661 aacgaggtcg tctaccgcag cacgcccacg tatcgcctct accagggccg cgacggggaa
     3721 tggttcttcc tggccgtcgg caaccaatcg ttctgggtga agctctgcaa agcggtcggc
     3781 cgcgaggacc tggcgcagga cccgcgcttc ggctcgtggt tctcccggcg cgacaacgcg
     3841 gacgcgctca tgccgctgct cgaggaggcg ttcggctcca agcccgcggc ggagtgggtg
     3901 cgcatcctga ccgcccacga catcccggcc gcggtgaccc agtcgctgcg ggagttcatg
     3961 cgcgatcccg cggtgctcca tcacaagatg atcgtcgagt acgaccaccc ggagctgggc
     4021 ccgctctccc tgatgggcca gccgctccga ttctccgaga cgcaggcccc cgacgcgggc
     4081 ccgccgccgc tcctcggcca gcacaccgcc gaggtcctgc gcgaggcggg cttcgccgac
     4141 cccgagatcg ccgagctgcg ccgccgcggc gtcgtcgccg gcaaggacat cccatgaccg
     4201 acgcaaagat cgacccggcg aggacgaagt acgaggccat ccgctacgag caggccgacg
     4261 gcgtggccac catcaccctg aaccggcccg acgtgcacaa cgcgatgaac caggcgatgc
     4321 ggcgcgagct gctcgaggtc ttcacgcgcc tgcgcaccga cgacacggtg cgcgcggtgg
     4381 tggtcaccgg cgggggcgac aaggccttct cggcgggcgc cgacatccgc gagttcctgg
     4441 agccgccggt gcccacccag ttccgcgagg cgcgccggcg cgtggacttc cgccgcgaga
     4501 tggagcgctg cccgcagccc atcatcgcgg ccatccgcgg ctacgccttc ggcggcgggc
     4561 tcgagctggc tctggcctgc gacatccgcg tcgccgccga ggacgcgcag ctcggcctca
     4621 ccgagatcaa cctggccatc atcccgggcg gtggcggcac ccagcgcctg ccgcggctcg
     4681 tgggacgcgg caaggcgctc gagatgatcc tcaccggcat gcgcattccc gccgcggagg
     4741 cgctgcgcat cggcctggtg gagcgcgtgg tcccgctcgc ggagcacccg tccgccgcgc
     4801 aggcgctggc ccgcaccatc gcggacaagg cgccggtggc gctgcgctac gcgaaggaag
     4861 cggtggtggg cggcatcggc ctcccgctgg aggacggcat ccgcctcgag aacgacctgg
     4921 ccaccctcct gcgcaccacc gaggaccgcc tcgaaggcgc caaggccttc gtggagaagc
     4981 gcaagccccg ctggaccggc aagtaacctt tcagcgcggg gtgtcgccgg ccagcgtcac
     5041 gcggtgcatg aggcgcttga agccggcgta gtcgttcacc gcgttgtgct ggacgcagcg
     5101 gttgtcccag aaggcgatgg agccgggctg ccactggaag cggcaggtga gctccgggcg
     5161 cacctgttgc cggaagagat agtcgaggag cggccggctc tcctcctccg tccagccct
//
LOCUS       DASUFA010000426        10043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_798959, whole genome shotgun sequence.
ACCESSION   DASUFA010000426 DASUFA010000000
VERSION     DASUFA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798959"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFX87_15390"
     CDS             <1..212
                     /locus_tag="VFX87_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996367.1"
                     /translation="KILDIHARTGNSHYVGVFSYAGMPYDEAERNLRLFAKEVMPQLQ
                     KLGTTVARAGRARAAEKPGVGLLGS"
     gene            complement(333..1109)
                     /locus_tag="VFX87_15395"
     CDS             complement(333..1109)
                     /locus_tag="VFX87_15395"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4996368.1"
                     /translation="MAAPRFDGKLALITGAAGGIGRAAAVRFAAEGARVGLVDVSREG
                     LRESLAAVEKVGGTGLTVEADVTRAADVTRYAAAVADRFGGIDCFFNNAGILGAVSPL
                     VDYPEDVFDQVLAVNVKGVWLGIKTVAPLLRARGGGVIVNTASIAGLRGSRNLVAYTA
                     SKHAVVGLTRSAALELAPDRIRVNAVCPSPIETDMVRLLEEGASPANPAAFHDRMAGV
                     IPMKRYGTPDEVAALVLFLCSADAVYITGAIYPVDGGSMA"
     gene            complement(1118..1465)
                     /locus_tag="VFX87_15400"
     CDS             complement(1118..1465)
                     /locus_tag="VFX87_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4996369.1"
                     /translation="MSIKRHGVGPRLSQAVVHGNTVYLAGLVAGDPSADAKGQTEQIL
                     KKIDDLLAATGSHKSKLLSATIYVANMGLYNDMNAAWDAWVDPANTPARATVEARLAS
                     PKYVVEIMVVAAI"
     gene            1576..1932
                     /locus_tag="VFX87_15405"
     CDS             1576..1932
                     /locus_tag="VFX87_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996370.1"
                     /translation="MDIRRAVGIAGLALLLLACEREEKMVREDLPMARATKARTDAQA
                     IATAVNTYRASCGGTLPESLEALTTQTMVGGAPCGPMLATIPTPPPGWSVYVYTRQGD
                     TFTVASSSGAQTVSAP"
     gene            1998..3059
                     /locus_tag="VFX87_15410"
     CDS             1998..3059
                     /locus_tag="VFX87_15410"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX4996371.1"
                     /translation="MTAITELETPAVTVHLDVMADNIRRVQAHLTRRGLGNRPHIKTH
                     KIPAIGQMQMAAGAVGITCQKLGEVEVFADAKVADDVLLTYNILGAAKTERLMALARR
                     VPRLAVVFDNEVVARELSAAAARHQVALEFLVECDTGFRRTGVQTPDEAFALARTAMR
                     LPGLQFKGLMTFPNRDPDTREFFERALALFKQNGIPVPVVSGGGTPALATVDRFPMLT
                     EHRAGTCVYNDAMVVSTGTATWEQCAMRVRATVVSRPTADRGVLDAGSKVLTSDLYHM
                     KGYGHVMEYPEAHVAHLSEEHAVVDFAGCAERPKVGEVVSVVPNHCCVVTNMVDEVYG
                     VRNAQVEVVWPVAARGRVQ"
     gene            3067..4602
                     /locus_tag="VFX87_15415"
     CDS             3067..4602
                     /locus_tag="VFX87_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4996372.1"
                     /translation="MTRTGAHLVVEALAAAGVRHLFSLSGNQILSLYDASLGSGLSIL
                     HTRHEAAAVHMADAWGRLTEEPGVALVTAGPGHCNAISALYGALMAESPVVLLSGHAP
                     TAQLGRGAFQEMDQAAVAAPVCKASWMAKDAARLGEDIATALELARSGRPGPVHVSLP
                     GDLLEAKVAAADGARAARREPPVAAAAAEVEQALALLAAARRPVIMMGGAMARGPRRA
                     AVARLGDATGVPALPMESPRGFNDPWLHLAAPLLGQADLLLLLGKKPDFVVRFAQPPA
                     VAAGCRVIQVDADAAARGRAWSHRAWGDEVARARATAPASWAEARRSARTPIHPLRVC
                     AALQPHLDRGAILVADGGEFGQWVQAGLEAETRLINGPAGSIGSAIPMAIAARLRHPD
                     RPVIAAVGDGTFGFHAFELDTAARYGLPVVTVIGNDARWNAEHQLQIQHYGADRTVGC
                     ELAPSRYDRLAAALGAHGEHVERAEDLDPALDRALRAGIPAVINVAIEGLPAPTYTTA
                     KSH"
     gene            complement(4665..5324)
                     /locus_tag="VFX87_15420"
     CDS             complement(4665..5324)
                     /locus_tag="VFX87_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX4996373.1"
                     /translation="MPPAGLLKRAAAGFGVLAGLAWAGAAPSSLVAAPAPAAIAQAGP
                     GLAGQFLVAADTLRDPRFARTVIYMVRHDASGAMGLVVNRPVRDMPLAPLLRKFGLDD
                     RGVTGSVRAHYGGPVDIGQGFLLHTAEYATEGTSLIAGDIAMTPTPGVLTALSDIARG
                     AGPRKSLFAVGYAGWAPGQLEAEIERGAWITVPADEALLFDEDHAGKWDRAMARRRIT
                     I"
     gene            5340..6590
                     /locus_tag="VFX87_15425"
     CDS             5340..6590
                     /locus_tag="VFX87_15425"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996374.1"
                     /translation="MALPSGLRALGHRDFRLFWCGQLVSLVGTWMQSVGQAWLVLELT
                     NSPFRLGLIGALQFGPILLFSFLSGAISDRVRKRRLLLGTQTALMLQAFTLAVLVWSG
                     HVQFWHVAVLAALYGLATTLDMPSRQSYIAHLVPRGDLMNAIALNSAVFNGARVVGPA
                     AAGLLVARYGTAAAFVMNGASFIAVIGALAAIRTEGAPGLRRGIGLRAEIAEGVRYAA
                     GTPRVALVMSLLVVVGLFVVNMSVLVPLIARDVLHEGAHGFGLLMASLGVGAVAGALA
                     VAALSVGRPPLGMVVGPALAAASLLLLLSTARHFGATAAVLMALGCAQIIFMTSCNTT
                     VQIAVPDELRGRVMGLYSLVFAGMTPIGALIMGMVAEHWGVSRACAVGGAAGLVLILA
                     LTGTWRRWHPAPAAPPPSSTPPAP"
     gene            6626..7741
                     /locus_tag="VFX87_15430"
     CDS             6626..7741
                     /locus_tag="VFX87_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4996375.1"
                     /translation="MDKSTDAQRRARPTPDVTSTADTVPVRETHRFDVAALERYMAQH
                     VARFKGPVTVSQFQGGQSNPTYRLSSPGGDYVLRRKPPGKLLPSAHAVEREYRIITAL
                     ARTPVPVPRTYALCEDDAVIGTAFFIMEWMSGRVMADPLLPGVSREDRGRIYDSMNEV
                     LARLHMVDLEATGLTDYGRPGSYFARQIHRWTTQYRASETERIEAMERLIAWLPEHVP
                     AEDRPTIVHGDFRPGNLIVHPTEPRVVAVLDWELSTLGSPLADLAYNCMPYRLAPSTL
                     GGVLGADLAALGLPTEADYVAAYCRRTGRSGIPDWEFYLAFAMFRLAAIAQGIMGRVI
                     AGTANDPNARSRGERARPLAEAGWETISQGMGSQGAG"
     gene            complement(7704..8432)
                     /locus_tag="VFX87_15435"
     CDS             complement(7704..8432)
                     /locus_tag="VFX87_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEX4996376.1"
                     /translation="MLLLAGGCMAFERTGQSWWELRRDSSGQAPDPAETRAAVVQVYA
                     ARTVGWRGVLAVHSWISVKPSGAPAYTRYEVMGWGVGNGAPAVRVNRTGPDNYWFGSR
                     PERLVDRRGDGVDELIARVQAAVEAYPYPTSYRTWPGPNSNTFIAYLGRAVPELRLDL
                     PATAIGKDFIPGGVPVAMSPSGTGAQVSLFGLLGVLAGVEEGVELNVLSLTFGVDVKR
                     PALKLPIVGRVGFSRLPDCPCPER"
     gene            complement(8465..8659)
                     /locus_tag="VFX87_15440"
     CDS             complement(8465..8659)
                     /locus_tag="VFX87_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996377.1"
                     /translation="MKSKSASKKKTSSVRAKAKPAKKPAAKAPAKSTAAKAAPAAPRP
                     APYTPKPIEGMGWAPFRYTR"
     gene            8732..9130
                     /locus_tag="VFX87_15445"
     CDS             8732..9130
                     /locus_tag="VFX87_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4996378.1"
                     /translation="MRLTKAQAKLIQWERVARVATVGAVGMPHLVPVCQLLLGGKIYF
                     ASGKKGRKVLNLRANLKLALTVDLYSDDWDRIQGVMVQGTARVIERGPRFRSLRRRLY
                     AKYPQYPREAALGESDSVIVELTPTHVFSW"
     gene            9301..9630
                     /locus_tag="VFX87_15450"
     CDS             9301..9630
                     /locus_tag="VFX87_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996379.1"
                     /translation="MRTAVNKRLTGFVVVLTLATAPAAMADDTKARQGAQQVESGAKQ
                     IGQGVQETARGIGKTVVGGAETAGERIKEAGRAAEPEAKSAWTKTKEGAVSFGHGVKN
                     FFTSLFD"
     gene            9686..9985
                     /locus_tag="VFX87_15455"
     CDS             9686..9985
                     /locus_tag="VFX87_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996380.1"
                     /translation="MKTTRVMSLVAATVFALGVAGSAVAQTPTTPTPAPAPAQPAAPA
                     KGGDMQMEKKVKKDGKAADKQHGKKKHTAKKAEGDMKPAPDMKAAPETKAPEVKK"
BASE COUNT         1411 a         3569 c         3643 g         1420 t
ORIGIN      
        1 agaagatcct ggacatccac gcgcgcaccg gcaacagcca ctacgtgggc gtgttcagct
       61 acgcgggcat gccctacgac gaggccgagc gcaacctgcg cctcttcgcc aaggaagtga
      121 tgccgcagct ccagaagctc ggtaccaccg tggcccgggc cggccgcgcc cgagccgccg
      181 agaagcccgg cgtcggcctc ctcggatctt agctcggagg ggggctgcgc cccccttccg
      241 aacctccccc caacgactga gtgcgcgggc gaagcccgcg ctcgaagcgg cggagttcag
      301 actgcgtgta gcctccgccg gtcggtaccc gatcaggcca tcgagccgcc gtcgacggga
      361 tagatcgcgc ccgtgatgta gacggcgtcg gcgctgcaga ggaagagcac cagcgcggcg
      421 acctcgtcgg gggtgccgta gcgcttcatg gggatcactc cggccatgcg atcgtggaag
      481 gccgccgggt tcgcggggct ggcgccctcc tcgaggaggc gcaccatgtc ggtctcgatc
      541 gggctcgggc agaccgcgtt gacgcggatg cggtccggtg ccagctcgag cgcggcgctg
      601 cgggtgaggc cgaccaccgc gtgcttgctg gcggtgtagg ccaccagatt gcgcgagccg
      661 cgcaggcccg cgatggacgc ggtgttgacg atgacgccgc cgccccgcgc gcggagcagc
      721 ggcgccaccg tcttgatgcc gagccagacg cccttgacgt tgaccgcgag cacctggtcg
      781 aagacgtcct ccgggtagtc caccagaggg ctgactgcgc ccaggatgcc cgcgttgttg
      841 aagaagcagt cgatgccgcc gaagcggtcg gcgaccgccg cggcatagcg cgtcacgtcg
      901 gcggcccggg tcacgtcggc ctcgacggtg agcccggtgc cgcccacctt ctccaccgcg
      961 gccagcgact cgcgcaggcc ctcgcgcgag acgtccacca gcccgacccg cgcgccctcc
     1021 gcggcgaagc gcaccgcggc ggcgcggccg atgccgccgg ccgcaccggt gatcagcgcg
     1081 agcttgccgt cgaagcgggg cgcggccatt gtcgccctca gatcgccgcg acgaccatga
     1141 tctcgaccac gtacttgggc gaggccagcc gcgcctccac ggtggcgcgg gccggcgtgt
     1201 tggccggatc cacccaggcg tcccaggccg cgttcatgtc gttgtagagg cccatgttgg
     1261 ccacgtagat ggtggcggag agcagcttgg acttgtggct gcccgtcgcg gccagcaggt
     1321 cgtcgatctt cttcaagatc tgctcggtct ggcccttggc gtcggcggac ggatcgccgg
     1381 ccacgaggcc ggccaggtag acggtgttgc cgtgaacgac ggcctgactc agtcggggtc
     1441 cgacgccgtg acgcttgatg ctcatcgcgc gctccttgtg gttgggggtg acttggctga
     1501 atacggtcgg gcggcgtatg attctgtcac acttcggttg gcgcgtcgga ttttctcacc
     1561 cggaaaggac ggacggtgga catccgcagg gcggtgggca tcgcggggct ggcgctgctg
     1621 ctgctcgcgt gcgagcgcga ggagaagatg gtgcgcgagg acctgccgat ggcccgcgcc
     1681 accaaggctc gcaccgacgc gcaggcgatt gccaccgcgg tcaacaccta tcgcgcgagc
     1741 tgcggtggga cgctgcccga gtcgctggag gcgctgacca cccagaccat ggtgggcggc
     1801 gcgccgtgcg gcccgatgct cgccaccatc ccgaccccgc cccccggctg gtccgtctac
     1861 gtctacacgc gccagggcga cacgttcacc gtggcctcga gcagcggcgc gcagaccgtc
     1921 tcggcgccat agcgctccat gtcccgcgtg acccacgaca cgtgttggcc gggagcgcgc
     1981 aggctgagga gggacccatg accgcgatca ccgagctcga gacgcccgcg gtgacggtgc
     2041 acctggacgt gatggcggac aatatccgcc gcgtgcaggc ccatctgacc cgtcgcggtc
     2101 tcggcaaccg cccgcacatc aagacgcaca agattccggc catcggccag atgcagatgg
     2161 cggcgggcgc ggtgggcatc acctgccaga agctgggcga ggtggaggtc ttcgccgacg
     2221 ccaaggtggc cgacgacgtg ctgctcacgt acaacatcct cggcgccgcc aagaccgagc
     2281 ggctgatggc cctcgcgcgg cgggtgcctc ggctcgcggt ggtgttcgac aacgaggtgg
     2341 tggcccgcga gctctccgcg gcggcggcgc gccaccaggt ggccctcgag ttcctcgtcg
     2401 agtgcgacac cgggttccgg cgcaccggcg tccagacgcc cgatgaggcc ttcgcgctgg
     2461 cccgcaccgc catgaggctc ccgggcctgc agttcaaagg gctcatgacg ttccccaatc
     2521 gcgaccccga cacccgcgag ttcttcgagc gggcactcgc cctgttcaag cagaacggca
     2581 tcccggtgcc ggtggtctcc ggcgggggga cccccgccct cgccaccgtg gaccgcttcc
     2641 cgatgctgac cgagcaccgc gcgggcacct gcgtctacaa cgacgcgatg gtggtgtcga
     2701 ccggtaccgc cacgtgggag cagtgcgcga tgcgcgtgcg cgccaccgtg gtgagccgcc
     2761 cgaccgccga ccgcggcgtg ctcgacgcgg ggagcaaggt gctcacctcg gacctctacc
     2821 acatgaaggg ctacggccac gtcatggagt acccggaggc gcacgtggcc catctctccg
     2881 aggagcacgc ggtggtggac ttcgcgggct gcgcggagcg gccgaaggtg ggcgaggtcg
     2941 tgagcgtggt gcccaaccac tgctgcgtgg tcaccaacat ggtggacgag gtctacgggg
     3001 tgcggaacgc ccaggtcgag gtggtctggc cggtggccgc gcgcggccgg gtgcagtgag
     3061 gccggcgtga cccggaccgg cgcgcacctg gtcgtggagg cgctggccgc cgccggcgtc
     3121 cggcatctgt tctcgctgtc cggcaaccag atcctctcgc tctacgacgc cagcctcggc
     3181 agtggcctct cgatcctgca cacgcggcac gaggcggcgg cggtgcacat ggcggatgcg
     3241 tgggggcggc tcaccgagga gccgggcgtg gccctggtca ccgccgggcc cggccactgc
     3301 aacgcgatct cggccctcta cggcgcgctg atggccgaat cgccggtggt gctgctgagc
     3361 gggcacgcgc ccaccgcgca gctgggccgc ggcgccttcc aggagatgga tcaggccgcg
     3421 gtggccgcgc cggtctgcaa ggcctcgtgg atggcgaagg acgcggcgcg cctgggcgag
     3481 gacatcgcga ccgcgctgga gctggcgcgg agcgggcggc ccgggcccgt gcacgtgagc
     3541 ctccccggcg atctgctcga ggcgaaggtg gccgcggccg acggcgcgcg ggcggcgcgg
     3601 cgcgagccgc cggtggcggc ggccgccgcg gaggtggagc aggcgctggc gctgctcgcg
     3661 gccgcccggc ggcccgtcat catgatgggc ggtgcgatgg cgcgcgggcc gcggcgcgcc
     3721 gccgtcgcgc ggctcggcga cgccaccggc gtgcccgcgc tgcccatgga gagcccgcgc
     3781 gggttcaacg acccgtggct gcacctggcc gccccgctgc tcgggcaggc cgatctgctc
     3841 ctgctcctgg gcaagaagcc ggacttcgtg gtccgcttcg cgcagcctcc cgcggtggcc
     3901 gcgggctgcc gcgtgatcca ggtggatgcg gacgcggccg cgcgagggcg ggcctggagc
     3961 catcgggcgt ggggcgacga ggtcgcgcgc gcgcgggcga cggcgccggc ttcgtgggcc
     4021 gaggcgcggc ggtcggcacg gacgccgatc cacccgctcc gggtctgcgc ggcgctccag
     4081 ccgcatctcg atcgcggggc catcctggtc gccgacggcg gcgagttcgg gcagtgggtg
     4141 caggcggggc tcgaggcgga gacgcggctg atcaatggcc cggcgggctc catcggcagc
     4201 gcgatcccga tggcgatcgc ggccaggctc cggcatcccg accgtccggt gatcgcggcg
     4261 gtgggcgacg gcaccttcgg cttccacgcc ttcgagctcg acaccgcggc gcgctacggg
     4321 ctgccggtgg tgacggtgat cggcaacgac gcgcgctgga acgcggagca tcagcttcag
     4381 atccagcact acggcgccga tcgcaccgtg ggctgcgagc tggcgccctc gcgctacgac
     4441 cggctcgcgg ccgcgctcgg cgcccacggc gagcacgtgg agcgcgcgga ggacctcgat
     4501 cccgccctcg atcgtgcgct acgcgcgggc atccccgccg tcatcaacgt cgccatcgaa
     4561 ggcctccccg cccccaccta caccaccgcc aagtcccact gacggccgat tggggtcagg
     4621 tcttacattc cgacaaaatg tcggaatgta agacctgacc ccggctagat ggtgatgcgg
     4681 cggcgggcca tggcgcggtc ccacttgccg gcgtggtcct cgtcgaagag gagggcctcg
     4741 tcggcgggca ccgtgatcca ggcgccccgc tcgatctccg cctcgagctg gcccggcgcc
     4801 cagcccgcgt agcccaccgc gaagagggac ttgcgcggcc ccgcgccgcg agcgatgtcg
     4861 ctcagcgcgg tgagcacgcc gggcgtgggc gtcatcgcga tgtcgcccgc gatgagcgaa
     4921 gtgccctccg tggcgtactc cgcggtgtgc agcaggaagc cctgcccgat gtcgaccggc
     4981 ccgccgtagt gcgcgcgcac gctgccggtg acgccacggt cgtcgaggcc gaacttgcgg
     5041 agcaggggcg ccagcggcat gtcgcggacg ggacggttga cgaccaggcc catcgccccg
     5101 ctcgcgtcgt gccggaccat gtagatgacc gtgcgcgcga agcgtggatc gcggagcgta
     5161 tccgcggcta cgaggaactg cccggcgagg cctgggccgg cctgcgcgat cgccgccggg
     5221 gccggggccg cgaccaggct cgacggcgcc gcgcctgccc acgcgagccc ggccagcacg
     5281 ccgaaccccg cggcggcgcg cttcagcaga ccggcgggag gcatggcaca gtataacgaa
     5341 tggcccttcc ctccgggctg cgcgccctcg gccaccgcga cttccgcctg ttctggtgcg
     5401 gccagcttgt ctccctcgtc ggcacgtgga tgcagagcgt cgggcaagcg tggctcgtgc
     5461 tcgagctgac caactctccg tttcgcctcg gcctgatcgg cgcgctgcag ttcggtccca
     5521 tcctgctctt ctcgttcctg tccggggcca tcagcgatcg ggtgcgcaaa cggcgcctgc
     5581 tcctcggcac gcagaccgcg ctcatgctgc aggccttcac actggccgtc ctggtctgga
     5641 gcggccacgt gcagttctgg cacgtggcgg tgctggccgc cctctacggg ctcgccacca
     5701 cgctcgacat gccgtcgcgc cagtcctaca tcgcccacct ggtgccccgc ggcgatctca
     5761 tgaacgccat cgcgctgaac tcggcggtgt tcaatggcgc ccgggtggtc ggccccgcgg
     5821 cggcggggct gctggtggcg cgttacggta ccgcggccgc cttcgtgatg aacggggcga
     5881 gcttcatcgc ggtgatcggg gcgctcgcgg ccatccgcac cgagggtgcg cccggcctgc
     5941 gccgcggcat tggcctccgg gccgagatcg cggaaggcgt gcgttacgcg gcgggcacgc
     6001 cccgcgtcgc gctcgtgatg agcctgctcg tggtagtggg tctcttcgtg gtcaacatga
     6061 gcgtgctggt gcccctgatc gcccgcgacg tcttgcacga gggcgcacac ggcttcggcc
     6121 tcctgatggc ctcgctcggc gtgggcgcgg tggcgggcgc gctcgcggtg gcggcgctgt
     6181 ccgtggggcg cccgcccctc gggatggtgg tgggcccggc gctggccgcc gcgtcgctgc
     6241 tgctcctgct ctccaccgcg cgccacttcg gcgcgaccgc ggcggtgctg atggcgctcg
     6301 gctgcgccca gatcatcttc atgaccagct gcaataccac cgtgcagatc gcggtgcccg
     6361 acgagcttcg ggggcgcgtg atgggtctct actccctcgt cttcgcgggg atgacgccga
     6421 tcggggcgct gatcatgggc atggtggccg agcactgggg cgtgtcccgg gcctgcgcgg
     6481 tgggcggcgc cgccgggctg gtgctgatcc tggcactcac cggcacgtgg cggcgctggc
     6541 atcccgcgcc cgccgcgccg ccgccgtcgt caactccccc ggcgccctga ccggccggtc
     6601 gtgccataat ccggactcgc ccgccatgga caagtcgacc gacgctcaac gccgcgcgcg
     6661 cccgaccccc gacgtgacct cgaccgccga taccgtgccg gtgcgcgaga cgcaccgctt
     6721 cgacgtggcc gcgctcgagc ggtacatggc ccagcacgtt gcgcgcttca aggggcccgt
     6781 cacggtgagc cagttccagg gcgggcagtc caacccgacc tatcgcctga gctcgccggg
     6841 gggcgactac gtgctgcgcc gcaagccgcc cggcaagctc ctgccctccg cccacgcggt
     6901 ggagcgcgaa taccgcatca tcaccgcgct ggcgcgcacc ccggttccgg tgccccgcac
     6961 ctatgccctc tgcgaggacg acgcggtgat cggcaccgcg ttcttcatca tggagtggat
     7021 gtcggggcgg gtcatggcgg acccgttgct gcccggggtg agccgggagg atcgcgggcg
     7081 catctacgac tcgatgaacg aggtgctcgc gcggctgcac atggtggacc tcgaggccac
     7141 cgggctcacc gactacggcc ggcccggcag ctacttcgcc cgccagatcc accgctggac
     7201 gacgcagtac cgcgcctcgg agacggagcg aatcgaggcg atggagcggc tgatcgcgtg
     7261 gctacccgag cacgtgcccg cggaggatcg gcccacgatc gtccacgggg acttccggcc
     7321 cggcaacctg atcgtccacc cgacggagcc gcgggtggtc gcggtgctcg actgggagct
     7381 gtccacgctc ggcagcccac tggccgatct ggcctacaac tgcatgccgt accggctcgc
     7441 ccccagcacc ctgggcgggg tgctcggggc cgatcttgcc gcgctcgggc tgccgaccga
     7501 ggcggactac gtggcggcct attgccgtcg caccggtcgc tcgggcattc ccgactggga
     7561 gttctacctg gccttcgcga tgttccgcct ggccgccatc gcccagggga tcatgggccg
     7621 cgtgatcgcg ggcaccgcca acgatcccaa cgcgcgctcg cggggggagc gggcgcggcc
     7681 cctggcggag gccggctggg agactatctc tcagggcatg ggcagtcagg gagccggctg
     7741 aacccgaccc gccccacgat cggcagcttc agcgccgggc gcttgacgtc caccccgaag
     7801 gtcaggctca gcacgttcag ctcgaccccc tcctccacgc cggccagcac ccccagcagc
     7861 ccgaagagcg agacctgcgc cccggtgccg ctggggctca tggccaccgg cacgccgccc
     7921 gggatgaagt ccttgccgat cgcggtggcg ggcaggtcca gccgtagctc cggcacggcc
     7981 cgccccaggt aggcgatgaa ggtattgctg ttgggccccg gccacgtacg gtagctggtg
     8041 gggtaggggt aggcctcgac cgcggcctgg acacgggcga tcagctcgtc aacgccgtcg
     8101 ccccgccggt ccaccaggcg ctcgggccgg ctcccgaacc agtaattatc cggcccggtc
     8161 cggttcaccc gtaccgccgg tgccccgttg ccgacgcccc agcccatgac ctcgtaccgc
     8221 gtataggccg gcgccccgct cggcttgacc gagatccagc tgtggacggc cagcacgccc
     8281 cgccacccca ccgtccgggc ggcgtagacc tggacgacgg cggcgcgtgt ctcggcggga
     8341 tccggagcct gcccggagga atcgcgccgc agctcccacc agctctggcc ggtccgctcg
     8401 aacgccatgc agcccccggc cagcaggagc aaggccccga gcgcagcccc ccaggccagg
     8461 cgggctaccg cgtgtagcgg aagggggccc agcccatccc ctcgatcggc ttcggggtgt
     8521 agggggcggg gcggggcgcc gcgggggcgg ccttcgcggc ggtgctcttg gcgggcgcct
     8581 tcgcggccgg cttcttggcc ggcttggcct tggcgcgcac ggacgacgtc ttcttcttgg
     8641 aagcgctctt gcttttcatg cggccgaatc tacatcaacg ctctcgtcgc ggtcaatgcg
     8701 tcgcatacac tcgcgccaag gagaacaacc catgcgactg acgaaggcgc aggcgaagct
     8761 gatccagtgg gagcgcgtgg cgcgcgtcgc cacggtgggc gcggtcggca tgccgcacct
     8821 cgtgccggtc tgccagctcc ttctcggcgg caagatctac ttcgcgtcgg ggaagaaggg
     8881 gcgcaaggtc ctcaacctcc gcgccaacct gaagctggcc ctgaccgtgg atctctactc
     8941 ggacgattgg gaccggatcc agggggtgat ggtgcagggc accgcacggg tgatcgagcg
     9001 gggcccgcgc ttccggagcc tgcgccggcg cctctatgca aagtatccgc agtacccgcg
     9061 cgaggcggcg ctgggcgagt ccgactcggt gatcgtggag ctcacgccga cgcacgtctt
     9121 ctcctggtaa tccttacacc ccgccgtgta attcctctcg gcctgctctc cacgcatgag
     9181 gggcaaacgc ccggttagcg cgcgattgcc tctgcggtgg cgccaccgcg gtggcacgtg
     9241 ggttgccctg aactttcccg cgagtcacgg cgttacaccc acgaagcaag gaggcaggag
     9301 atgaggacgg cggtgaacaa gcggctcacg ggattcgtcg tggtgctgac gctggcgacg
     9361 gcaccggccg ccatggccga cgacaccaag gccaggcagg gcgcgcagca ggtggagagc
     9421 ggcgccaagc agatcggcca gggtgtccag gagacggccc ggggcatcgg caagaccgtg
     9481 gtcggcggcg ccgagacggc gggcgagagg atcaaggagg cggggcgggc cgcggagccc
     9541 gaggccaagt cggcctggac caagaccaag gaaggggccg tatcgttcgg ccacggcgtg
     9601 aagaatttct tcacgagcct gttcgactaa ccagcggcaa acagacctca accagatctc
     9661 aacgcagaga ggggagttac tcgatatgaa gacgacccgt gtgatgtctc tcgtggcggc
     9721 aacggtgttc gcgctcggcg tggccggctc ggcggtggct cagaccccga ccactcccac
     9781 cccggcgccg gcgcccgcgc agcccgcggc ccctgcgaag ggcggcgaca tgcagatgga
     9841 gaagaaggtc aagaaggacg gcaaggcggc cgacaagcag cacggaaaga agaagcatac
     9901 ggccaagaag gccgagggcg acatgaagcc ggcgcccgac atgaaggcgg cgcccgagac
     9961 gaaggcgccc gaagtcaaga agtaggaagt gtccagccgc gcgagggatc gcgcgttcac
    10021 ataggcgcgg cggcgcgggg gga
//
LOCUS       DASUFA010000427        15025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1705863, whole genome shotgun sequence.
ACCESSION   DASUFA010000427 DASUFA010000000
VERSION     DASUFA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 15025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15025
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705863"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFX87_15460"
     CDS             <1..685
                     /locus_tag="VFX87_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996381.1"
                     /translation="KGRFRGSPVTASGGYIAGLLGRHLDGPARVSLEAPIPVDHPFSI
                     ERLADGRVVLSDGAAVLAHAEAATLQLEPPTPPSFAEAQWASERYLGFTEHAAPECFV
                     CGPAREWGDGLAIFPGPLPGRRLVAAPWAPDPTLFDKRGVVRPEFVWAALDCPIGFAL
                     LEAFGRRKAVLNQLTASLIRPLEVGARWIVMGWPFAADGRNLLGASAIVSESGELHAL
                     ASAVLVCAD"
     gene            complement(687..1151)
                     /locus_tag="VFX87_15465"
     CDS             complement(687..1151)
                     /locus_tag="VFX87_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4996382.1"
                     /translation="MTAGDVYFEDFTPGREFRTEGITVSEGQIIDFAMAFDPQPFHLD
                     VEAAKATIYEGLIASGFQTMALTFRLFVQTRALAACSLGSPGVDEVRWLRPVRPGDTL
                     RATVQVLEQRASTSKPDRGIVRLHWTTINHRGEPVLSMTSMQLVRRRPAAGA"
     gene            complement(1151..2095)
                     /locus_tag="VFX87_15470"
     CDS             complement(1151..2095)
                     /locus_tag="VFX87_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEX4996383.1"
                     /translation="MARVITVGAAQLGPIARQEGRGLVVKRLLDLLGQAHGRGCDLVV
                     FPELALTTFFPRWWMEDQAEIDAFFEREMPGSETRPLFDEAARLGMAFCLGYAELIRE
                     GGALRHYNTSILVDRAGRLVGKYRKVHLPGHAAHEPWRPFQHLEKRYFDVGDLGFPVW
                     RALGGVLGMCICNDRRWPETYRVMALQGVEMILLGYNTPVHNPPAPEQDPLGPFHNHL
                     VMQAGAYQNGTWVVGVAKCGREEGCDLIGQSAIIAPSGELVAQAVTLGDELITARCDL
                     DLTRSYKDTIFNFARHRRPEHYRLIVDRVGAEPPPEER"
     gene            complement(2106..2441)
                     /gene="trxA"
                     /locus_tag="VFX87_15475"
     CDS             complement(2106..2441)
                     /gene="trxA"
                     /locus_tag="VFX87_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX4996384.1"
                     /translation="MTNENEWLQHLDERNYDEALVAAEGVMMVDFWAEWCGPCRAIGP
                     VLEDLAKSSGGAVTLAKVNVDENPGLAARYGIRSIPTILFVKDGKVRDQVVGAVPKTQ
                     IQAKLASLA"
     gene            2518..3252
                     /locus_tag="VFX87_15480"
     CDS             2518..3252
                     /locus_tag="VFX87_15480"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4996385.1"
                     /translation="MGRLPMPSIRLDHIAIALPRIADAPSVLVAVLGGVPERSQPSGA
                     FRWASWTYADGGRIEILEPTGAEGFLHRFLAAHGPGVHHVTFKVPDLGAVCRRAERHG
                     YGIVGRDESDPSWKEAFFHPKQALGIVVQLAEAGGPPGAPGDVPPGLPGAPPPVRILG
                     LRMRAHSRDRALTQWRDILEAEVEDGPAAGLAFRWPGSPMRVLVDVDPTAAEGPLAIE
                     LASARALAGLEEAGARLGIRLAQRSA"
     gene            3283..4203
                     /locus_tag="VFX87_15485"
     CDS             3283..4203
                     /locus_tag="VFX87_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX4996386.1"
                     /translation="MDFLTTDLRALLIGLAGSTAQTAGRLVLVLVAGYFGARVLRTLI
                     DQLENMLVVAGERTGALPGVTRSRVATLTSVLRTLALVGLWSLVVVICLSQLGFDVRP
                     ILAGAGIVGLAVGFGAQHLVRDVIAGFFLVLEDQVRVGDVAVVNGTGGLVETVTFRTI
                     VLRDLAGTVHIFPNGNVTTLANMTKDWSGYVVDVEVGYREDPDRVVALMRRVFEELRQ
                     DPTHGPLIIAPIEIFGVDAFKDSTVVIKARLKTVPIQQWTVGREYRRRLLLAFAAESV
                     EVPPTRVVQIGDAGKPFPVLLMQGAAPVPR"
     gene            4333..5361
                     /locus_tag="VFX87_15490"
     CDS             4333..5361
                     /locus_tag="VFX87_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX4996387.1"
                     /translation="MNLGGLERVFSSDLAVDLGTSNTRLYVRGQGVVLDEPSVVAVDM
                     NGAVQAVGNDAKAMLGRAPGSVQVVRPLRHGVIADFDRTQKMFGYFINKTRRRWSFVQ
                     PRLVIVTPSGITQVERRAVRDAAQQAGAREVYLVQASMAAALGAGLPVEQPGASMIVD
                     VGGGTTEVAVMSLCGIVYSESLRTAGDEMNQAIVHYLRRAYDLLVGERRSEEAKIRLG
                     SAYPKGDDHEPMEVQGRDLLDGLPKTVVVTALEVREALREPVTDIIMAVRACLEHTPP
                     ELAADISDNGIVLTGGGALLRGLDELLHQETGLSIRVTEDPLTCVARGAGMILDQLGI
                     LKRVAIPA"
     gene            5434..7107
                     /locus_tag="VFX87_15495"
     CDS             5434..7107
                     /locus_tag="VFX87_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996388.1"
                     /translation="MIARVPFLLAVAVFLAMGCSAQPGSHPHHGGSTAAPAAGTPVLF
                     DNLGPYHRAVTTSSRQAQAYFDQGLRLVYGFNHYEAQAAFREAARLDPTCAMCAWGIA
                     LTHGSNYNSPTDAERERGAWAAVSQARTLAAVRGTEPERALIAALAARHSASPTADRA
                     TLDRAYADAMRDVTRRFPADLDAATLFADALMNLRPWSLWTPDGKPQPGTEEIVAVLE
                     RVLAADPRHPGANHLYIHAVEASPDPRRAEAAADRLRGLMPGAGHMVHMPSHIYYRIG
                     RYADAGSVNVAAVAADRAYFTKSQPSPIYRGMYYPHNLDFVWHAAGMEGRSAECVRAA
                     REFASEVPTAAVLEMPDMETAPAAPLLALARFGRWDEVLAQPAPPAKMLYVTATWRYA
                     RGLAFTAKGRRDEAARELAEIRAVLARVPADRTLAGFFKMADMVRLASETLAGEMAAR
                     GGDAEAAVKHLALAVRLQDEHWFTEPPPWHYPVRQSLGAALLQAGRAAEAEAVYREDL
                     RWNPENGWSLFGLAQSLRAQGKTADAAAVDARFSRAWARADVSLTSSRF"
     gene            7114..7353
                     /locus_tag="VFX87_15500"
     CDS             7114..7353
                     /locus_tag="VFX87_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996389.1"
                     /translation="MIQDRYGLSLSTSSAAAGRAYVEGVDRFLAAHAAAEECFDQSVA
                     ADPGFAPAHIGRARSLQLLLARRVDRQPSVPVAGA"
     gene            7353..7838
                     /locus_tag="VFX87_15505"
     CDS             7353..7838
                     /locus_tag="VFX87_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996390.1"
                     /translation="MAEPVREYRYQVDRDGRIFHDGSEIVDPAVLRFFLRAMTRTADG
                     CCLVLCQGERNWFEAADTPFVIQRLRLAIEGGLLQDVELCFPGDYREPLDPRTLETER
                     DLLFCRVRGGAFRARFGRVAVQQLAPFLSDAGEGSALLLGGVRHPVPSGAAVPRPAGP
                     R"
     gene            7841..9637
                     /locus_tag="VFX87_15510"
     CDS             7841..9637
                     /locus_tag="VFX87_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996391.1"
                     /translation="MPPSGRRSRFTTYWTLAALATVMLSACGGAGAAGLGSAVNASEP
                     REPVAEPATFHSLGVRWAVRGDANADAVIGVRYRPRAETGWREALPLFRTDPDSVSDE
                     NRVKGGWLFAGSIVDLAPDTEYEVALALTDPDGGGVQRTLYMKTGAEPREPAGMRVRH
                     VVPAGAADRGPGTGAATDPVRGLRIAQAAAMPGDLFLLHAGVYGEGTWTIDRHGTPGR
                     PIIYRGAGDGETILDGGGRERLVSATGVKHVWLERLTLRRARYLFVGHSGSHFVVRRC
                     RFEVVGTGITAINGGYLESRGFVITDNVFQGPTRWPRSQGIEEVNGVSITGAGHVVAY
                     NRMSNLGDGVHGTGHGRLSASDIYNNDVEASTDDGIEGDYADTNVRIFRNRITNAFSG
                     ISAQPSHGGPLYVFRNAIYNVLYSPFKLHNDTSGVLIFHNTSVLNGIPWHIEPGGETV
                     SDVVTRNNLFVGTSGPALRSTGDMIRCEFDRDGYGGAGGPGGAAHVFAEWNGRVFATP
                     EEARRSGALYRRYGAVLVPARGNFASGLGAPEDSDTRFPREKTDLRLDPASRAVDAAV
                     VLPNFNEGFSGKAPDLGCCELGQPLPHYGPRP"
     gene            9670..10572
                     /locus_tag="VFX87_15515"
     CDS             9670..10572
                     /locus_tag="VFX87_15515"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4996392.1"
                     /translation="MADRVGFIGLGTMGMPMATSLAKGGVPLVVHDASPAALAAAAKL
                     PGAIAAKSVAEAAAQATVLFTCLPNDTIVREVYLGPDGIASAARAGLVTCDCSTVSPE
                     VTTAVHAALKGRGVDHMDTPMLGSQPQAVEGQIFFIVGGEKARLPTIAPYLEIMGKMH
                     MYVGGPSMANRVKLIHNGLAAVTSVAVAEALAVCVQSGVDPGTFYDVVRNGGGMAYGT
                     YFDRRAKRIFEGEFSPTFMLELMRKDAMLALALARSVQVPAPMLEETKRTYDEAVDSG
                     WGREDFSAVTHVVEKRIGRRLSGR"
     gene            10660..11127
                     /gene="bcp"
                     /locus_tag="VFX87_15520"
     CDS             10660..11127
                     /gene="bcp"
                     /locus_tag="VFX87_15520"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX4996393.1"
                     /translation="MSLVEPGRKAPAFSLPDQEGRTHRLKDYAGRPLIVYFYPKDDTP
                     GCTKEACAFRDALPDFKKGKAAVLGVSVLDTASKAKFARKHKLAFPLLADPEHEVADR
                     YGAWRKKSLYGRTYMGVARMTYLIGPDGKVLRRWDNVKVDGHAADVLRAVREL"
     gene            11135..11992
                     /locus_tag="VFX87_15525"
     CDS             11135..11992
                     /locus_tag="VFX87_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX4996394.1"
                     /translation="MTAVIVGAYEDAATAIAALAPAGGKRRAAVLPRAAVDDEARVRL
                     RQAQVECLSTLDGDDLRAARGLATALAEAEGWERADGAPSPTGQSEPGDVIGWYEVRI
                     GSGALTTDRPITRLHGSRRYIASFNLLGQARLNQACGDLLYRGLMDEGVALGEVFDVV
                     VCSESKAVGMVQVLVECFGQDRYVVLRKGVKNYMPRHPRAPLVEEASSITTAGAQALV
                     LDPLDWPLLEGRRVLLVDDVIATGGTARAACRLLERAGAHVTVAATVLLKGPEPDLPR
                     LVVLARPLL"
     gene            12024..12692
                     /locus_tag="VFX87_15530"
     CDS             12024..12692
                     /locus_tag="VFX87_15530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4996395.1"
                     /translation="MGTMTTLTAEDGHRLSAYRAAPSGTPKGGLVVVQEIFGVNHHIQ
                     SMCDRFAADGYVALAPAIFDRVEPGINLGYTADDIEQGRSIRGKVDIADMVRDVRAAV
                     EALKAEGQSVGVVGYCLGGTLAWLACTRIDGIKAAVGYYGGGIAETASETPRCPVLLH
                     FGETDQGIPPEHWARIRAAHPEVPLHTYPAGHGFSCDERGSFHKPSADLARQRTLGFF
                     QALL"
     gene            complement(12746..13726)
                     /locus_tag="VFX87_15535"
     CDS             complement(12746..13726)
                     /locus_tag="VFX87_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX4996396.1"
                     /translation="MTTYEAHELALIFPPMSEPEFAAFKEDIREQGQQEAIILYEGKI
                     LDGLHRYRACQELGREPRVVRFEGNPRAAAQLVLGRNFHRRHLTASQRALVAAEMCKL
                     RPRGNTGMSPYLTAAQASTLMSVGEDLVKDAKHLLRHGDEELIRSVRDGVRSVSAAIQ
                     EKKEKARFTFIEQFAQRLDRAIQGHNDRDAQVVYGAKPLLQYITLRIQDYLDPRTAEP
                     LGGEDGRVLWDPAELKVPDWLYTERGEATAAERRARLAAVLPKARDAAMDFPGERCSA
                     CGLPLLSTMRPAEPSYCAVCKGNVLWEWSWMREQVEVAFSSALRSIAHPA"
     gene            13901..14380
                     /locus_tag="VFX87_15540"
     CDS             13901..14380
                     /locus_tag="VFX87_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4996397.1"
                     /translation="MASKYTCTVRPEWIDNNGHMNMGYYVVVFDLATDEWMRLVGLDA
                     AHRRAREVTTFCLEAHVTYHREVREGDPLRFTTRLLAHDTKRVHYIHEMFHGEAGYLA
                     ATNELMSLHVSQATRRGAPMAPEILERLGEIQRAHDRLPRPPQVGRVMGLGSRPTTR"
     gene            14456..>15025
                     /locus_tag="VFX87_15545"
     CDS             14456..>15025
                     /locus_tag="VFX87_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX4996398.1"
                     /translation="MTRRRALWTSLLLLLVIGLALPPGAPAQWAVSSNDNKVVLDNGV
                     VKVVPNAAPDTVTIIDLGVTPPKVIAEIPVPGSVVGPPMSVAITPDQGLALVTSSMKK
                     DPADATKTIPDNRVSVVDLRATPPAVIATLEAGKGASGVSINRQGTLALVANRAEGTV
                     SVFTISGKTVTPVGKVTVGDAKSGVSHAAF"
BASE COUNT         2084 a         5315 c         5394 g         2232 t
ORIGIN      
        1 gaagggtcgt ttccgcggct cccccgtgac cgcgagcggc ggctacatcg ccggactgct
       61 cgggcgccac ctcgacggcc cggcgcgcgt gagcctcgag gccccgatcc cggtggacca
      121 tcccttcagc atcgagcggc tcgcggacgg ccgggtggtt ctctccgacg gtgcggccgt
      181 cctcgcccac gcggaagcgg ccacgctgca gctcgagccg cccacgcctc cgtccttcgc
      241 ggaagcgcag tgggcctccg agcgatatct cggcttcacc gagcacgccg cccccgagtg
      301 cttcgtgtgc gggccggccc gggagtgggg cgacgggctc gcgatcttcc ccgggccgct
      361 cccgggtcgg cgtctggtcg ccgcgccctg ggcgccggat ccgacgctct tcgacaagcg
      421 cggggtcgtg cgtcccgagt tcgtctgggc cgccctcgat tgcccgatcg gcttcgccct
      481 gctcgaggct ttcggccgcc gcaaggcggt gctgaaccag ctcaccgcga gcctgatccg
      541 acccctcgag gtcggcgcgc ggtggatcgt gatgggctgg cccttcgcgg cggacggccg
      601 caacctgctc ggcgcgtccg cgatcgtgtc ggagagcggc gagctgcacg cgctcgcctc
      661 ggcggtgctg gtctgcgcgg actgactcag gccccggcgg cggggcggcg tcggacgagc
      721 tgcatgctgg tcatgctgag caccggctca ccgcggtggt tgatcgtggt ccagtgcagc
      781 cgcacgatgc cgcggtcggg cttggacgtc gacgcccgct gctccagcac ctgcaccgtc
      841 gcccgaagcg tatcgccggg acgcactggc cggagccacc gcacctcgtc cactcccggc
      901 gagcccaggc tgcaagcggc caacgcgcgg gtctggacga agaggcggaa ggtcagcgcc
      961 atggtctgga agccgctcgc gatgagcccc tcgtagatcg tcgccttggc ggcctcgacg
     1021 tcgagatgaa agggctgcgg atcgaaggcc atcgcgaagt cgatgatctg cccttcgctg
     1081 accgtgatcc cctcggtgcg gaactcccgt cccggcgtga agtcctcgaa gtagacgtcg
     1141 cctgcggtca tcaacgctcc tcgggcggcg gctcggcgcc cacgcggtcc acgatcaggc
     1201 ggtagtgctc cggccgccgg tggcgggcga aattgaagat cgtgtccttg taggagcgtg
     1261 tgagatcgag atcgcagcgc gcggtgatga gctcgtcgcc gagcgtgacg gcctgggcga
     1321 cgagctctcc ggacggcgcg atgatcgcgc tctggccgat caggtcgcag ccttcttccc
     1381 ggccgcactt ggccacgccg accacccacg tgccgttctg ataggcgccg gcctgcatca
     1441 ccaggtgatt gtgaaagggg ccgagcgggt cctgctccgg cgcgggcggg ttgtgcaccg
     1501 gcgtgttgta gccgagcagg atcatctcga ctccctgtag cgccatgact cgatacgtct
     1561 ccggccagcg ccggtcgttg cagatgcaca tgccgagcac gccgcccagc gcgcgccaga
     1621 ccggaaagcc gaggtcgccc acgtcgaagt agcgcttctc cagatgctgg aacgggcgcc
     1681 acggctcgtg cgcggcgtgg ccggggagat ggaccttgcg gtacttgccc acgagacgcc
     1741 cggcgcggtc caccaggatc gacgtgttgt agtgccggag ggcgccgccc tcccggatca
     1801 gctcggcgta gcccaggcag aacgccatcc cgagccgcgc cgcctcgtcg aagagcggcc
     1861 gcgtctcgga accgggcatc tcccgctcga agaaggcgtc gatctcggcc tgatcctcca
     1921 tccaccagcg cgggaagaag gtggtcaggg cgagctcggg aaagacgacg aggtcgcagc
     1981 cgcgcccgtg ggcctgcccg agcaggtcca ggaggcgctt gaccaccagg ccccggccct
     2041 cctgccgcgc gatgggcccg agctgcgcgg ccccgaccgt gatgacgcgg gccatcccgc
     2101 cgcgatcagg cgaggctcgc cagcttggcc tggatctgcg tcttcgggac cgcgcccacc
     2161 acctggtcgc ggaccttgcc gtccttcacg aacaggatgg tcgggatcga ccggatgccg
     2221 tagcgggccg cgaggcccgg gttctcgtcc acgttcacct tcgccagggt cacggcgccg
     2281 ccggagctct tggccaggtc ctccaggacc ggtccgatgg cccggcaggg accgcaccac
     2341 tccgcccaga agtccaccat catcacgcct tcggcggcca ccagcgcctc gtcgtagttg
     2401 cgctcgtcga ggtgttggag ccattcgttt tcgttggtca tcgctctgtc ctcccgccgt
     2461 ttagatggcg ctaccgggtc cggcgatgct cgggctggac cggcttccga gagtaagatg
     2521 ggaaggctgc cgatgccctc gatccggctc gatcatatcg cgattgccct gccgcgcatc
     2581 gccgacgctc cttccgtcct ggtggcggtg ctgggagggg tccctgagcg aagtcagccc
     2641 tccggtgcgt tccggtgggc aagctggacc tacgcggacg ggggccgcat cgagatcctg
     2701 gagcccacgg gcgcggaggg ctttctccac cggttcctgg cagcgcacgg cccgggtgtc
     2761 catcacgtca ccttcaaggt gccggacctg ggcgcggtct gccggcgggc cgagcgccac
     2821 ggctacggta tcgtcggtcg cgacgagtcc gatccctcgt ggaaggaagc cttctttcac
     2881 cccaagcagg ccctcggcat cgtggtgcag ctcgccgagg cgggggggcc gcccggtgct
     2941 ccgggtgacg tcccgccggg gctgcccggt gcgccgccgc cggtgcgcat tctgggcctg
     3001 cgtatgcgcg cccactcccg cgatcgcgcc ctcacgcagt ggcgcgacat tctggaggcc
     3061 gaagtcgagg acggaccggc ggccgggctc gccttccgct ggcccggctc gccgatgcga
     3121 gttctcgtgg acgtcgatcc gaccgctgcg gagggcccgc tcgccatcga gctcgcgagc
     3181 gcgcgcgcgc tcgcgggtct cgaggaggcg ggcgcgcgcc tgggcatccg tctcgcgcag
     3241 cggtctgcct gatcttcaga ttccacccaa ggaggcaagc cgatggactt tctcacgacc
     3301 gatctccgag ccctgctgat cggcctggcc ggatccaccg cgcagaccgc gggtcggctg
     3361 gtgctcgtgc tggtcgccgg ctacttcggc gcccgcgtcc tgcgcacgct gatcgaccag
     3421 ctcgagaaca tgctcgtcgt ggcgggcgag cggaccggcg cgctgcccgg ggtcacgcgc
     3481 tcgcgcgtgg ccacgctcac gagcgtgctg cgcaccctgg ccctggtcgg gctctggtcg
     3541 ctggtggtcg tcatttgcct ctcccagctc ggcttcgacg tgcgaccgat cctggccggt
     3601 gcgggcatcg tcggcctggc ggtgggcttc ggggcgcagc acctggtgcg cgacgtcatc
     3661 gccgggttct tcctcgtgct cgaggaccag gtgcgggtgg gcgacgtcgc ggtggtcaac
     3721 ggtaccggcg gcctcgtgga gaccgtgacg ttccgcacca tcgtgctgcg tgacctcgcg
     3781 ggcaccgtgc acatcttccc gaacggcaac gtgaccacgc tcgccaacat gaccaaggac
     3841 tggtcgggct acgtggtgga cgtggaggtg ggttaccgcg aggatcccga tcgagtcgtc
     3901 gccctcatgc ggcgcgtgtt cgaggagctc cggcaggatc ccacccacgg tccgctgatc
     3961 atcgcgccca tcgagatctt cggggtggac gccttcaagg actcgaccgt ggtgatcaag
     4021 gcccggctca agacggtccc catccagcag tggaccgtcg gccgtgagta ccggcggcgc
     4081 ctgctcctgg ccttcgcggc cgaaagtgtc gaggtgccgc cgactcgggt cgtccagatc
     4141 ggcgacgccg gcaagccctt cccggtgctc ctgatgcagg gcgcggcgcc agtgccgcgc
     4201 tgaggcgcaa tccgatgaca gccgccgtca ctcgggcgcc gagagagtct cgtaacatgc
     4261 tgatttaact ggtgtcgatc gtggtccgct ttttgaactg cctgggggtc ctggcgggag
     4321 ggccacgatc gaatgaatct tggcgggctg gagcgggtgt tctcgagcga tctggcggtt
     4381 gacctgggca cgtccaatac ccggctctac gtccggggcc agggcgtggt cctcgacgag
     4441 ccgtccgtgg tggcggtgga catgaacggg gccgtgcagg ccgtgggcaa cgacgccaag
     4501 gccatgctcg gtcgggcccc cgggtcggtg caggtggtcc ggcccctccg ccacggcgtc
     4561 atcgccgact tcgatcgcac ccagaagatg ttcggctact tcatcaacaa gacccgccgg
     4621 cgctggagct tcgtgcagcc gcgcctggtg atcgtcaccc cctccggcat cacccaggtc
     4681 gagcggcggg ccgtccggga cgcggcccag caggccgggg cgcgcgaagt ctacctcgtg
     4741 caggcgtcca tggccgcggc cctcggcgcg ggcctgccgg tggagcagcc gggcgccagc
     4801 atgatcgtgg acgtgggcgg cggcaccacc gaggtcgcgg tcatgtcgct gtgcgggatc
     4861 gtctactcgg agtcgctgcg caccgcgggc gacgaaatga accaggcgat cgtccactac
     4921 ctccggcgcg cctacgacct gctcgtcggg gagcggcggt ccgaggaagc caagatccgg
     4981 ctgggctccg cctatcccaa gggcgacgac cacgagccga tggaagtgca gggccgcgac
     5041 ctgctcgacg gcctgccgaa gaccgtggta gtcaccgctt tggaagtccg cgaagcgctg
     5101 cgcgagccgg tgaccgacat catcatggcg gtgcgcgcct gcctcgagca caccccgccc
     5161 gagctggccg ccgacatcag cgacaacggc atcgtgctca cgggtggcgg cgccctcctg
     5221 cgtggactgg acgagctcct gcatcaggag accgggctca gcatccgggt gaccgaggat
     5281 ccgctcacct gtgtcgcccg gggcgccggc atgatcctgg accagctcgg gatcctgaag
     5341 cgcgtcgcaa tccccgccta gctcccgctg gttgccgcca gcctctggcg gagataccct
     5401 cggggcgatg tccacttccc gaggaggccg gcgatgatcg cgcgtgtgcc cttcctgctg
     5461 gcggttgccg tgttcctggc gatgggttgc tcggcgcagc ccggatcgca ccctcaccat
     5521 ggcggttcca ccgcggcgcc ggccgcgggg acgccggtgc tcttcgacaa cctcggcccc
     5581 tatcatcgcg cggtcaccac gtcgtcgcgg caggcgcagg cctacttcga ccaggggttg
     5641 cggctcgtgt acgggttcaa ccactacgag gcccaggccg ccttccgtga ggcggcgcgg
     5701 ctcgatccca cgtgcgcgat gtgcgcgtgg ggcatcgcgc tgacccacgg ctccaactac
     5761 aacagcccca ccgacgccga gcgcgagcgc ggcgcgtggg ccgcggtgag ccaggcccgc
     5821 acgctcgcgg ccgtgcgtgg caccgagccg gagcgggcgc tcatcgccgc gctcgccgcg
     5881 cgccactcgg cctcgccgac cgcggatcgc gccacgctcg accgcgcgta cgcggacgcg
     5941 atgcgcgacg tgacccggcg cttcccggcc gatctcgacg cggccaccct cttcgccgac
     6001 gccctcatga acctccgccc ctggagcctc tggacgccgg acggcaagcc gcagccgggc
     6061 accgaggaga tcgtcgcggt cctcgagcgc gtcctcgccg ccgatccgcg gcatccgggc
     6121 gccaatcacc tctacatcca cgcggtggag gcgagccccg atccgcgccg cgccgaggcc
     6181 gccgcggacc ggctgcgcgg gctgatgccc ggcgcgggcc acatggtcca catgccgtcg
     6241 cacatctact accggatcgg gcggtacgcc gacgcgggct ccgtcaacgt ggccgcggtg
     6301 gcggcggatc gcgcctactt cacgaagagc cagccgagcc cgatctatcg gggcatgtac
     6361 tatccgcaca atctcgactt cgtctggcac gcggcgggca tggagggtcg gagcgccgag
     6421 tgcgtgcggg ccgcgcggga gttcgcctcc gaggtaccga ccgccgcggt gctcgagatg
     6481 ccggacatgg agacggcgcc ggccgcgccg ctgctcgcgc tggcgcgctt cggccgctgg
     6541 gacgaggtgc tggcgcagcc cgcgccgccc gcgaagatgc tctacgtgac cgcgacctgg
     6601 cgctatgccc gcgggctggc cttcaccgcc aagggtcgcc gcgacgaggc cgcgcgcgag
     6661 ctggccgaga tccgggcggt gctcgcgcgc gtgccggccg accggacgct ggccggcttc
     6721 ttcaagatgg ccgacatggt gcgcctggcc tcggagacgc tggccggcga gatggcggcg
     6781 cgcggcgggg acgccgaggc ggcggtgaag cacctggccc tcgcggtgcg cctgcaggac
     6841 gagcactggt tcacggagcc gccgccgtgg cactaccccg tgcgccagtc tctcggcgcc
     6901 gcgctgctgc aggccggccg cgccgccgag gcggaggcgg tgtatcgcga ggatctccgc
     6961 tggaatcccg agaacggctg gtcgctgttc ggcctcgccc agagcctgcg cgcccagggg
     7021 aagacggccg acgccgccgc ggtggacgcg cgcttcagtc gcgcctgggc gcgcgcggac
     7081 gtctcgctca cctcgtcgcg cttctagcgg ccgatgatcc aggatcgcta cggcctctcg
     7141 ctctcgacat cgtccgcggc cgcgggccgg gcgtacgtgg agggcgtgga ccggtttctc
     7201 gcggcccacg ccgccgccga ggagtgcttc gatcaatcgg tcgccgcgga tcccggcttc
     7261 gcgccggccc acatcgggcg ggcgcggagc ctccagctcc tgctcgcccg tcgggtggat
     7321 cgccagccgt cggtcccggt ggcgggcgcc tgatggcgga gccggtccgc gagtaccgct
     7381 accaggtgga tcgcgacggc cggatcttcc acgacggcag cgagatcgtg gacccggcgg
     7441 tgctgcgctt cttcctgcgg gcgatgacca ggaccgccga cgggtgctgc ctcgtgctgt
     7501 gccagggcga gcggaattgg ttcgaggcgg cggacacgcc cttcgtgatc cagcggctgc
     7561 gcctggccat cgaagggggt ctcctccagg atgtcgagct gtgtttcccc ggcgactacc
     7621 gcgagccgct cgatccgcgc acgctcgaga ccgagcgcga cctgctcttc tgccgggtcc
     7681 gcggcggcgc cttccgcgcg cgcttcggcc gtgtcgcggt ccagcagctc gctccgttcc
     7741 tgtccgacgc gggcgagggc tccgcgctgc tcctgggcgg tgtccggcac ccggtgccca
     7801 gcggcgccgc cgtcccgcgg ccggccggtc ctcgctagcg atgccgccgt ccggtcggcg
     7861 gtcccgcttc acgacgtact ggacgctggc cgcgctcgcc acggtgatgc tcagcgcctg
     7921 cgggggcgcg ggggccgccg gactcggctc ggcggtcaac gcgtccgagc ccagggagcc
     7981 ggtggccgag cctgccacct tccactcgct gggggttcgc tgggcggtgc gaggcgacgc
     8041 caacgccgac gcggtgatcg gggtgcggta ccgcccgcgg gccgagacgg gctggcggga
     8101 ggcgctgccg ctcttccgga ccgatcccga ctccgtctcc gacgagaatc gggtcaaggg
     8161 cggatggctc ttcgcgggga gcatcgtgga cctcgctccc gacaccgagt acgaggtggc
     8221 gctcgcgctg accgacccgg acggcggcgg cgtgcagcgc acgctgtaca tgaagaccgg
     8281 cgccgagccc cgggagccgg ccggcatgcg cgtgcggcac gtggtgccgg cgggtgcggc
     8341 ggaccgcggc ccgggaaccg gcgccgcgac ggatcccgtc cgcggcctgc gaatcgcgca
     8401 ggccgccgcg atgccgggtg atctctttct cctgcatgcg ggcgtgtacg gcgagggcac
     8461 ctggaccatc gaccgtcacg gcactcccgg gcggccgatc atctaccgcg gagcgggcga
     8521 cggcgagacg atcctcgacg ggggcgggcg cgagcggctg gtcagcgcga ccggcgtgaa
     8581 gcacgtgtgg ctggagcgcc tcaccctgcg tcgcgcccgg tacctcttcg tcgggcattc
     8641 gggctctcac ttcgtggtcc ggcgctgccg gttcgaggtc gtgggcaccg ggatcaccgc
     8701 catcaacggc ggctatctcg agtcccgggg attcgtcatc accgacaacg tgtttcaagg
     8761 ccccacccgc tggccccgca gtcagggcat cgaggaggtc aacggcgtgt ccatcaccgg
     8821 ggccggccac gtggtggcct acaaccggat gagcaacctc ggggacgggg tccacgggac
     8881 cgggcacgga cggctatcgg cgagcgacat ctacaacaac gacgtcgagg cgtccaccga
     8941 cgacgggatc gagggcgact acgcggatac caacgtccgg atcttccgta accgcatcac
     9001 caacgcgttt tccggaatca gcgcccagcc ctcccacggg ggtccgctct acgtgtttcg
     9061 caacgcgatc tacaatgtgc tctactcgcc gttcaagctc cacaacgaca cctccggcgt
     9121 gctgatcttc cacaacacgt cggtgctgaa cggcatcccg tggcacatcg agccgggcgg
     9181 cgagacggtg agcgacgtgg tgacgcggaa caatctcttc gtgggcacca gcggcccggc
     9241 cctgcgatcg acgggcgaca tgattcgctg cgagttcgac cgcgacggct acgggggtgc
     9301 cggcggtccc ggcggggcgg cccacgtctt tgcggaatgg aacggccggg tgttcgccac
     9361 ccccgaggag gcgcggcgct ccggggcgct ctaccgccgc tacggcgccg tgctggttcc
     9421 cgcgcgcggc aacttcgcga gtgggctcgg tgcgcccgag gacagcgaca cccggtttcc
     9481 gcgcgagaag accgacctgc gcctcgaccc ggcctcccgg gcggtggacg ccgccgtcgt
     9541 gctgcccaac ttcaacgagg ggttctcggg caaggcaccc gatctcggct gttgcgagct
     9601 cggacaaccg ctgccccact acggcccgcg tccctgacgg gcgcgaggcg aggaccccga
     9661 ggagcgatca tggctgaccg cgtcggattc atcggactgg gcacgatggg catgccgatg
     9721 gcgaccagcc tggccaaggg cggcgtgccg ctggtggtgc acgacgcgag cccggccgcg
     9781 ctggctgccg ccgcgaagct cccgggggcg atcgcggcca agtcggtcgc cgaggccgcg
     9841 gcccaggcca cggtgctctt cacctgcctg cccaacgaca cgatcgtgcg cgaggtgtat
     9901 ctgggaccgg atggcatcgc ctccgccgcg cgcgcgggcc tcgtcacctg cgactgctcg
     9961 acggtgagcc cggaggtgac gaccgcggtg cacgccgcgc tcaagggccg cggcgtggat
    10021 cacatggaca cgcccatgct gggctcgcag ccgcaggcgg tggaggggca gatcttcttc
    10081 atcgtgggcg gcgagaaggc gcgcctgccg accatcgcgc cctatctcga gatcatgggc
    10141 aagatgcaca tgtacgtggg cggaccgagc atggccaatc gcgtgaagct gatccacaac
    10201 gggctcgccg cggtcacctc ggtggcggtg gcggaggcgc tcgcggtctg cgtgcagtcc
    10261 ggcgtggatc ccggcacgtt ctacgacgtg gtgcgcaacg gcgggggcat ggcctacggc
    10321 acctacttcg accgacgcgc caagcgcatc ttcgagggcg agttctcgcc gaccttcatg
    10381 ctcgagctga tgcggaagga cgcgatgctc gcgctggccc tggccagatc cgtgcaggtg
    10441 cctgcgccga tgctggagga gaccaagcgc acctacgacg aggcggtgga cagcggctgg
    10501 ggccgcgagg acttctcggc ggtgacccac gtggtggaga agcgcatcgg gcggcggctc
    10561 tcgggccggt agcgcggccg cccgccgggt agtaggatga gcgcccggcg cgcggtcggg
    10621 cgccggctcc actcatcgtc gccgaccagg aggtctccga tgtcgctcgt cgagccgggg
    10681 cgcaaggcgc ccgcgttctc cctgccagat caggaaggcc ggacgcaccg gctcaaggat
    10741 tacgcgggcc gtccgctgat cgtgtacttc taccccaagg acgacacccc cggctgcacc
    10801 aaggaggcct gcgctttccg cgacgcgctg cccgacttca agaagggcaa ggcggccgtc
    10861 ctcggggtga gcgtgctgga cacggcgagc aaggccaagt tcgcccggaa gcacaagctc
    10921 gccttcccgc tgctggccga tcccgagcac gaggtggccg accgctacgg ggcgtggcgg
    10981 aagaagagcc tctacggccg cacgtacatg ggggtggccc ggatgacgta cctcatcggg
    11041 cccgacggca aggtgctccg ccgctgggac aacgtgaagg tggacggcca cgccgccgac
    11101 gtgctccgcg cggtccgcga gctctgagcg gacggtgacc gcggtcatcg tcggggccta
    11161 cgaggacgcc gcgaccgcca tcgcggcgct cgcgcccgcg ggtggcaagc gccgcgccgc
    11221 cgtcttgcca cgcgccgcgg tggacgacga ggcgcgcgtg cggctgcggc aggcgcaggt
    11281 cgagtgcctc agcacgctgg acggggacga tctgcgcgcg gcccgggggc tggccaccgc
    11341 gctcgcggag gccgaaggat gggagcgggc cgacggcgcg ccgtcgccca cggggcagag
    11401 cgagcccggc gacgtgatcg gctggtacga ggtgcgcatc ggcagcggcg ccctcaccac
    11461 cgaccgaccc atcacccggt tgcacggctc gcggcgttac atcgcctcgt tcaacttgct
    11521 ggggcaggcg cggctcaacc aggcgtgcgg cgacctgctc tatcgcgggc tcatggacga
    11581 gggagtggcg ctcggggagg tcttcgacgt ggtggtctgc tcggagtcga aggcggtcgg
    11641 catggtgcag gtgctggtgg agtgcttcgg ccaggaccgc tacgtggtgc tgagaaaagg
    11701 cgtcaagaac tacatgccgc gccatccccg cgcgccgctg gtggaggaag ccagcagcat
    11761 caccaccgcg ggcgcgcagg cgctggtgct ggatccactg gactggccgc tgctggaggg
    11821 ccgtcgcgtg ctgctggtgg acgacgtgat cgccaccggc ggcaccgccc gcgccgcctg
    11881 ccggctgctg gagcgcgcgg gcgcccacgt caccgtcgcc gcgacggtgc tgctgaaagg
    11941 ccccgagccc gacctgccgc gcctcgtggt cctggcgcgg ccgctgctgt gatcgccgtt
    12001 caaccccgac aaggagcgtg acgatgggca ccatgacgac cctcaccgct gaagacggac
    12061 accggctgtc ggcctaccgc gcggccccga gcggcacgcc caagggcggc ctcgtggtcg
    12121 tgcaggagat attcggggtc aaccaccaca tccagagcat gtgcgaccgc ttcgcggccg
    12181 acggctacgt ggcgctggcg cccgccatct tcgaccgcgt ggagcccggc atcaacctcg
    12241 gctacaccgc cgacgacatc gagcagggcc ggtcgatccg gggcaaggtg gacatcgccg
    12301 acatggtccg cgacgtgcgg gccgcggtgg aggcgctcaa ggccgaaggg cagagcgtgg
    12361 gcgtggtggg ctactgcctg ggcggcacgc tcgcgtggct ggcgtgcacg cgcatcgacg
    12421 gcatcaaggc ggcggtgggc tactacggcg gcggcatcgc ggagaccgcg agcgagacgc
    12481 cgcgctgccc ggtcctgctg cacttcggcg agaccgacca ggggattccg cccgagcact
    12541 gggcccgtat ccgcgccgcg catccggagg tgccgctaca tacgtacccg gccggccacg
    12601 gcttcagctg tgacgagcgg ggaagcttcc acaagcccag cgccgacctg gcccgccagc
    12661 ggacgctcgg gttcttccag gcgctgctct agcggcgacg atcgggcggg ggcggggggg
    12721 ccgatgttct cccccgcccc cgcgcctagg cgggatgtgc gatggagcgg agggcgctgc
    12781 tgaacgccac ctccacctgc tcgcgcatcc aggaccattc ccagagcacg ttgcccttgc
    12841 agaccgcgca gtacgagggc tccgcggggc gcatcgtgga caggagcggc agcccgcagg
    12901 ccgagcagcg ctccccgggg aagtccatcg cggcgtccct tgccttcggc aggacggcgg
    12961 ccagccgggc acgccgctcc gccgcggtcg cctcaccccg ctcggtgtag agccagtcgg
    13021 ggaccttgag ctcggccggg tcccacagga cgcggccgtc ctcgcccccc aggggctccg
    13081 ccgtccgagg gtcgagatag tcctggatgc gcagcgtgat gtactggagg agcggcttgg
    13141 ccccgtacac cacctgggcg tcccggtcgt tgtggccctg gatcgcgcgg tcgaggcgct
    13201 gggcgaactg ctcgatgaag gtgaaccggg ccttctcctt cttctcctgg atcgccgccg
    13261 agaccgagcg gaccccgtcg cggaccgagc gaatcagctc ctcatcgccg tgcctcaaca
    13321 ggtgtttggc atccttgacc aggtcctcgc cgacgctcat cagggtggac gcctgggccg
    13381 cggtcaaata gggggacatc cccgtatttc cccggggccg gagcttgcac atctcggcgg
    13441 cgaccagggc ccgctgcgag gccgtgaggt gccgtcgatg gaagttccgg cccagaacca
    13501 gctgagccgc cgcgcggggg ttgccctcga accgcaccac ccgcggctcc cggccgagct
    13561 cctggcaggc gcgatagcgg tgcaggccat cgaggatctt cccctcgtac aggatgatcg
    13621 cttcctgctg cccctgctcg cggatgtcct ccttgaaggc ggcaaactcg ggctcgctca
    13681 tgggcgggaa tatcagggcg agctcgtggg cttcgtaggt ggtcatggcg gcgtcacctc
    13741 caggcctcaa gcatagaagc ccggaattta tcggggaatc ccctggagag ggcggggggc
    13801 tgcttgaccc cgccacggat gcggctatcc tgcgacggca ggaaggcccg attccaaggt
    13861 tcgtacccgg gagggagcca tcgccgaggc ggccgacgtc atcgcatcga agtacacgtg
    13921 tacggtccgc ccggagtgga tcgacaacaa cgggcacatg aacatgggct actacgtggt
    13981 ggtcttcgac ctggccaccg acgagtggat gcggctcgtc ggcctggacg ccgcgcatcg
    14041 ccgcgcgcgg gaagtgacca cgttctgcct ggaggcccac gtgacctatc atcgcgaggt
    14101 ccgcgaaggc gacccgctcc gattcaccac gcggctcctg gcgcatgaca cgaagcgcgt
    14161 tcactacatc cacgagatgt ttcacggcga ggccgggtat ctcgcggcca ccaacgagct
    14221 gatgtcgctg cacgtgagcc aggcgacccg gcggggcgcg cccatggccc ccgagatcct
    14281 cgagcgcctg ggcgagatcc agcgcgcgca cgaccggctg ccgcggccgc cgcaggtggg
    14341 ccgggtgatg gggctgggct cgcgtccgac gacgcggtag cggggccgac cgccgccgta
    14401 gtatggtcct cgccagttca cgaccggccc cctccaacac ctaggggagg tacccatgac
    14461 acgacgccgt gcgctctgga cctcgctcct gctgcttctc gtgatcgggc tcgcgctgcc
    14521 gcccggcgcc ccggcccagt gggcggtctc gtcgaacgac aacaaggtcg tgctcgacaa
    14581 cggggtggtc aaggtggtgc ccaacgcggc gcccgacacc gtgacgatca tcgatctggg
    14641 cgtgacgccg ccgaaggtca tcgcggagat cccggtgccc ggcagcgtgg tcggtccgcc
    14701 gatgagcgtg gcgatcacgc cggatcaggg gctggcgctc gtgacctcct ccatgaagaa
    14761 ggatccggcc gacgccacca agaccattcc cgacaatcgc gtcagcgtgg tggatctccg
    14821 cgcgacgccg cccgcggtga tcgcgacgct cgaggcgggc aagggcgcgt cgggcgtgtc
    14881 gatcaaccgg cagggcacgc tcgcgctcgt ggccaaccgc gccgagggca ccgtgtccgt
    14941 cttcaccatc agcggcaaga ccgtcacccc cgtgggcaag gtcaccgtgg gcgacgcgaa
    15001 gtcgggcgtg agccacgccg ccttc
//
LOCUS       DASUFA010000428         5590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_330167, whole genome shotgun sequence.
ACCESSION   DASUFA010000428 DASUFA010000000
VERSION     DASUFA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330167"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VFX87_15550"
     CDS             complement(<1..1516)
                     /locus_tag="VFX87_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4996399.1"
                     /translation="MLETLQNLALGFQVALSPGVLWYGFIGCLVGTLVGVLPGVGPLA
                     GISLLLPVTYGLDATRALVMLAGIYYGAMYGGSTTSILMRIPGEAASVMTCIDGYAMA
                     RKGRAGPALTIAAVGSYVAGTVSVIALMFLAPPLAAFALRFGPPEYFALLLLGLLVLA
                     YMNTGSMLKGLAMAALGLLLGMIGIDQMSGYFRFAYGVTELADGIGVVPVAVGLFGLA
                     EILLTAGQENPPAVIRPRLRDLLPSRQEWREAHLPIWRGTGIGFLIGIIPGSAHVIST
                     FVSYAVERKVSRHPEEFGRGAVAGVAGPESANNAATSGAFVPMLALGVPGVPIAALLL
                     AAMMIHGVTPGPLLIQQDPKLFWGFIASMYVGNAILLVLNLPLVGLFVNLLRVPYPVL
                     YPLIVVCSILGVYAVNSSVVDVWIMLAMGMLGYVLRKLDFETAPIVLGLVLAPMMELS
                     LRQSLAMSAGNYAIFVTRPISVTLLAVGAVLVVLALRAFITRSLDWRSRLPAETE"
     gene            complement(1520..1960)
                     /locus_tag="VFX87_15555"
     CDS             complement(1520..1960)
                     /locus_tag="VFX87_15555"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4996400.1"
                     /translation="MTVDRVAGVALVLIGLVTIWESRVFPLGSLHRPGPAYMPVVLAV
                     LLILFGAAVFAMDARVRRLAEVGWPEWRHALAIFGACAFAAWGLERLGYRLTMAVVVA
                     FLLLVVERKGWALGLALTVVMAWGSFFVFDTLLRVPLPRGPFGL"
     gene            complement(1975..2952)
                     /locus_tag="VFX87_15560"
     CDS             complement(1975..2952)
                     /locus_tag="VFX87_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4996401.1"
                     /translation="MRRALSGLGLFVLLTAIAVGPAWAQEPYPTRPVSIVVAFPPGGL
                     ADNTARPVAAALERILKQPVPIINKAGAAGAVGNQVVATSKPDGYTLLMALVSVSVLP
                     EVDKLFGRPQNYTLDQLTGIARINADPSMLVVRADAPWKTLKDLVEDAKKRPGEIVFT
                     SSGLYGAAHIPTEMFIKAAGIKMRHLPTTGGGPMMNAMLGGHAQVVMTPVSLAAQHVK
                     QGKLRLLAHSGTAPVAAFPEVPSFKAQGYDVEYTAWAGLVAPKGTPPHIIKILRDAVR
                     QAVKEPEVVSSHAKLETPIAYMDADEFNAWWAKDAARLAEVVKQIGKVE"
     gene            3067..3366
                     /locus_tag="VFX87_15565"
     CDS             3067..3366
                     /locus_tag="VFX87_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996402.1"
                     /translation="MAANGKLTVLNPVGFAPRITRKELALRLDTLHGKTVYLVDCRFD
                     DSDVFLQQMQAWLAEHMPAVKTIVKPISSVYLKDDPATWQEIKARGHAAIVGVGH"
     gene            3400..4716
                     /locus_tag="VFX87_15570"
     CDS             3400..4716
                     /locus_tag="VFX87_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX4996403.1"
                     /translation="MTIEAAYGVPTVALHTDKFDRVVRAVAAQNGMPGLRQAFVPQPV
                     MGKTAAELRAYVDGRDPISGRPVMQEVIEGLTRPFTDDERGAIDFDRSAPRLVEPDTE
                     EALHRLFLDRDWTDKLPIVLPTEARVAAMLAGTRRRPDEVVGRMRPTHFREAWEYTVE
                     KVAVNAVMAGAVPAYFPVILALAATGVSARGSSSSSLATMAVVNGPVRHEIGMNVGTG
                     ALGPHNHANATIGRAYGLLSQNLQGGSVPGLTYMGSMGNNYAYNSVTFGENEERSPWE
                     PFHVQHGFRPTDSAASVFTGCRSTAFTLGLRERYWREHVRNMFRGMDPHTPPTLLLDP
                     ITARQFVDRGGFLKKDALIDWLYDNARMPAGEYWDYQLVQNYIYPRATFGEEPWASKL
                     KAAPEELIPMFRREDIHVVVVGGETNGYWRIMGCVYQTTLSVDAWR"
     gene            4728..>5590
                     /locus_tag="VFX87_15575"
     CDS             4728..>5590
                     /locus_tag="VFX87_15575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX4996404.1"
                     /translation="MKLSVEFPSVSFREGPAAVAGLARAIERIGYDHIDIFDHVVMGV
                     PIEGRPRGPYNATMPILEALMALSYIAAVTTRVTLGTEVLVLPQRQPTLVAKQVSTLD
                     TLSGGRVRLGVGVGWQESEYEALGEPFRTRGARMDEAVRLLRAYWTDAEVSFESAHYP
                     TVSMAMEPKPPQGSRLPIWIGGNSEAALRRVGRLGDGWLASRVTDAAGAREAIEAIHR
                     HAEAAGRDPGAIGLQSMVAPPPRDAAGKTFYADHDAVVARVAALKAMGFGWVALHATA
                     IFQAGARSVAA"
BASE COUNT          904 a         2017 c         1947 g          722 t
ORIGIN      
        1 cctccgtctc ggccggcagc cgcgaccgcc agtcgagtga tcgagtgatg aacgcccgca
       61 gcgccaggac cacgagcacc gcccccaccg cgagcagcgt cacggagatg ggccgcgtca
      121 cgaagatcgc atagtttccc gcggacatcg cgagcgactg gcgcagcgac agctccatca
      181 tcggggcgag caccagcccg agcacgatgg gcgccgtctc gaagtcgagc ttgcgcagga
      241 catatccgag catgcccatg gcgagcatga tccacacgtc gaccacgctg ctgttgacgg
      301 cgtagactcc caggatcgag cacacgacga tgagcggata cagcaccggg tacggcacgc
      361 gcagcaggtt cacgaagagc ccgaccagcg gcaggttgag gacgagcagg atggcattgc
      421 ccacgtacat cgaggcgatg aagccccaga agagcttcgg gtcctgttgg atcaagagcg
      481 gccccggggt cacgccgtgg atcatcatgg ccgcgaggag gagcgccgcg atgggcacgc
      541 cgggcacgcc cagcgccagc atgggcacga aggcgccgga ggtggccgcg ttgttggccg
      601 actccgggcc cgccacgccg gccaccgcgc cccggccgaa ctcctccggg tgcctcgaga
      661 ccttgcgctc gaccgcgtag gacacgaacg tcgagatcac gtgcgcggag cccgggatga
      721 tgccgatgag gaacccgatc ccggtgccgc gccagatcgg caggtgcgcc tcgcgccact
      781 cctgacgcga cggcagcagg tcgcgcagcc gggggcggat caccgcgggc ggattctcct
      841 gccccgcggt gagcaggatc tcggcgagcc cgaagagccc gaccgccacc ggcaccacgc
      901 cgatgccgtc cgccagctcg gtcacgccgt aggcgaagcg gaagtagccg ctcatctggt
      961 cgatgccgat catcccgagg agcaggccca gggccgccat ggccaggccc ttcagcatcg
     1021 agccggtgtt catgtaggcg aggacgagga ggcccagcag caggagggcg aagtactccg
     1081 gcggcccgaa gcgcagcgcg aaggcggcca gcggcggcgc gaggaacatg agcgcgatca
     1141 cgctcaccgt ccccgccacg taggagccga ccgcggcgat ggtcagggcc ggccccgcgc
     1201 ggcccttgcg ggccatcgcg tagccgtcga tgcacgtcat caccgaggcg gcctcgcccg
     1261 ggatgcgcat caggatcgag gtggtcgagc cgccgtacat ggccccgtag tagatgccgg
     1321 ccagcatgac gagcgcccgc gtggcgtcga ggccgtaggt cacgggcagc agcaggctga
     1381 tgcccgcgag cgggcccacc cccggcagca cgcccaccag ggtgccgacc aggcagccga
     1441 tgaagccgta ccagagcacc ccggggctga gggccacctg gaagcccagg gccaggttct
     1501 ggagcgtctc gagcaacggt cagagcccga agggcccgcg gggcagcggc acgcgcagca
     1561 gggtgtcgaa gacgaagaag gagccccagg ccatcacgac cgtgagcgcg agcccgagcg
     1621 cccagccctt gcgctccacg accagcaaca ggaaggccac cacgaccgcc atggtgagcc
     1681 ggtagccgag ccgctcgagc ccccacgccg cgaacgcgca ggcgccgaag atggcgagcg
     1741 cgtggcgcca ctccggccag cccacctcgg ccaggcggcg aacgcgcgcg tccatcgcga
     1801 acaccgccgc gccgaagagg atcagcagca cggccagaac caccggcatg taggcgggcc
     1861 cgggccggtg gagcgagccg agcgggaaga cccggctctc ccagatcgtg acgagcccga
     1921 tcagcaccag cgccacgccg gcgacgcggt cgacggtcat ggcgcgcccg ggcgctactc
     1981 caccttgccg atctgcttca ccacctccgc gagccgggcc gcgtccttgg cccaccacgc
     2041 gttgaactcg tcggcgtcca tgtaggcgat cggggtctcg agcttggcgt gcgagctgac
     2101 cacctccggc tccttcaccg cctggcggac cgcgtcgcgc aggatcttga tgatgtgcgg
     2161 cggggtgccc ttcggggcca ccagccccgc ccacgcggtg tactcgacgt catagccctg
     2221 ggccttgaac gaggggacct ccgggaacgc ggccaccggc gcggtcccgc tgtgggcgag
     2281 cagccggagc ttgccctgct tcacgtgctg ggcggccagg ctcaccggcg tcatcaccac
     2341 ctgggcgtgg ccgccgagca tcgcgttcat catcggccct ccacccgtgg tcggcaggtg
     2401 ccgcatcttg atgccggccg ccttgatgaa catctcggtg gggatgtgcg ccgccccgta
     2461 gaggccggag gaggtgaaga cgatctcgcc gggccgcttc ttggcgtcct ccaccagatc
     2521 cttgagcgtc ttccagggcg cgtcggcccg gaccaccaac atggacggat cggcgttgat
     2581 ccgggcgatg ccggtgagct gatcgagcgt gtagttctgc ggacgcccga agagcttgtc
     2641 gacctcgggc aggaccgaga cgctgacgag ggccatcagc agcgtgtagc cgtccggctt
     2701 gctggtcgcg acgacctggt tgcccaccgc gcccgccgcg ccggccttgt tgatgatcgg
     2761 gaccggctgc ttgaggatgc gctcgagcgc cgccgccacc ggccgcgccg tgttgtcggc
     2821 caggcccccg ggcggaaacg cgaccacgat ggagaccggc cgcgtgggat acggctcctg
     2881 ggcccacgcc ggacccaccg cgatcgcggt cagcagcacg aacagaccca gccccgagag
     2941 cgctcgcctc atgctcatct cctttgccca tctccttcga tggaagtgtg ccgaagccta
     3001 gcagccggtc cccgagcagg tcaaggcgcg ctcgttgtag actgtcggcc acggaggcag
     3061 cccatcatgg cggcgaacgg caagctcacg gtcctgaacc cggtcggctt cgctcccagg
     3121 atcacccgca aggagctggc ccttcgcctc gacacgctgc acggcaagac ggtctacctg
     3181 gtggactgcc gcttcgacga ctcggatgtc ttcctccagc agatgcaggc ctggctcgcc
     3241 gagcacatgc cggcggtgaa gaccatcgtc aagccgatct cgagcgtgta tctcaaggac
     3301 gatccggcga cctggcagga gatcaaggcg cgcgggcacg ccgccatcgt cggcgtcggc
     3361 cactgaagca cctgcgcgcc ggcggtcgcc gcgcacgcga tgacgatcga ggccgcgtac
     3421 ggggtcccca ccgtcgccct gcacaccgac aagttcgacc gcgtggtccg cgccgtcgca
     3481 gcccagaacg gcatgccggg ccttcgccag gcgttcgtgc cccagccggt catgggcaag
     3541 acggcggcgg agctgcgcgc ctacgtcgac gggcgcgacc cgatcagcgg gcggccggtc
     3601 atgcaggagg tgatcgaggg gctgacccgg ccgttcacgg acgacgagcg cggcgcgatc
     3661 gacttcgacc ggtccgcgcc ccggctggtc gagcccgaca ccgaggaggc gctccaccgc
     3721 ctcttcctcg accgcgactg gacggacaag ctgcccatcg tgctccccac cgaggcgcgc
     3781 gtggccgcga tgctcgcggg cacccggcgc cggcccgacg aggtggtcgg gcgcatgcgg
     3841 ccgacccact tccgggaggc gtgggagtac accgtcgaga aggtcgcggt gaacgcggtc
     3901 atggccgggg cggtccccgc gtacttcccg gtgatcctgg ccctcgccgc gaccggggtg
     3961 agcgcgcgcg gcagcagctc gagctcgctc gccaccatgg cggtcgtcaa cgggccggtg
     4021 cgccacgaga tcggcatgaa cgtcggcacc ggcgccctcg gcccccacaa ccacgccaac
     4081 gcgacgatcg gccgcgccta cgggctcctc tcgcagaacc tccagggcgg ctcggtcccg
     4141 ggcctcacct acatgggctc catgggcaac aactacgcgt acaacagcgt gaccttcggc
     4201 gagaacgagg agcggagccc ctgggagccc ttccacgtcc agcacggctt ccggccgacc
     4261 gacagcgcgg ccagcgtgtt caccggctgt cgctcgacgg ccttcacgct cgggctgcgg
     4321 gagcggtact ggcgcgagca cgtgcgcaac atgttccgcg gcatggaccc gcacaccccg
     4381 ccgaccctcc tgctggatcc catcaccgcg cggcagttcg tcgatcgggg cgggttcctg
     4441 aaaaaggatg cgctcatcga ctggctctac gacaatgcgc ggatgccggc cggcgagtac
     4501 tgggactacc agctcgtgca gaactacatc tatccccgcg ccaccttcgg cgaggagccg
     4561 tgggcgtcca agctcaaggc ggcgccggag gagctgatcc cgatgttccg tcgcgaggac
     4621 atccacgtgg tggtcgtcgg cggcgagacc aacggttact ggcggatcat gggctgcgtg
     4681 taccagacca cgctctccgt cgacgcgtgg cgctagagaa aggactcatg aagctgagcg
     4741 tcgagtttcc gagcgtgtcc ttccgggagg gcccggccgc ggtggccggc ctcgcccgcg
     4801 ccatcgagcg gatcggctac gaccacatcg acatcttcga ccacgtggtc atgggggtgc
     4861 cgatcgaggg ccgcccccgc ggcccctaca acgcgacgat gccgatcctg gaggcgctga
     4921 tggcgctctc ctacatcgca gccgtcacca cgcgcgtcac cctcggcacc gaggtgctcg
     4981 tgctcccgca gcgccagccc acgctggtcg ccaagcaggt gagcacgctc gacacgctgt
     5041 cgggcgggcg ggtgcggctc ggcgtgggcg tcggctggca ggagtcggag tacgaggccc
     5101 tgggggagcc cttccggacg cggggcgcgc gcatggacga ggccgtccgc ctgctgcgcg
     5161 cctactggac cgacgccgag gtgagcttcg agagcgcgca ctaccccacc gtgtccatgg
     5221 ccatggagcc caagccgccg caagggtccc gcctgccgat ctggatcggc ggcaactcgg
     5281 aggccgccct gcggcgcgtc ggccggctcg gcgacggctg gctggccagc cgggtcaccg
     5341 acgcggccgg cgcgcgcgag gccatcgagg ccatccatcg ccacgccgag gcggccgggc
     5401 gcgatccggg ggccatcggc ctccagagca tggtggcgcc gccgccccgc gacgccgcgg
     5461 gcaagacgtt ctacgccgac cacgacgcgg tggtggcccg cgtcgccgcg ctcaaggcca
     5521 tgggcttcgg ctgggtcgcg ctccacgcca ccgcgatctt ccaggcgggc gcacgctccg
     5581 tggcggcgat
//
LOCUS       DASUFA010000429         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_541753, whole genome shotgun sequence.
ACCESSION   DASUFA010000429 DASUFA010000000
VERSION     DASUFA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541753"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFX87_15580"
     CDS             complement(<1..235)
                     /locus_tag="VFX87_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX4996405.1"
                     /translation="MITVKRLTLEDAKVLLAAAEAKAREIEVTETICVCDDGGHPIAL
                     HRMTGARLTGVEIAVAKAFTAAGHRRPTHRFNEP"
     gene            complement(232..555)
                     /locus_tag="VFX87_15585"
     CDS             complement(232..555)
                     /locus_tag="VFX87_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX4996406.1"
                     /translation="MTGAREVRVAALTDLPAGALRPVEVDGTRIVLVRVGDAVHACGD
                     VCPHRGGWLSEGKLSGMRLACPLHGWIFDVRSGQCLFPGRGARVPSYPVRIDGDSVFV
                     EVPAE"
     gene            complement(558..1241)
                     /locus_tag="VFX87_15590"
     CDS             complement(558..1241)
                     /locus_tag="VFX87_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX4996407.1"
                     /translation="MSSASPFRTQLEDAVNARHSRINPFTEKWVNGELTRAQLGAWVC
                     QHYQYVSQFARWCATIYGNCPDSDARDFLLENIIEEESGTKHVDLLIRFGEACGVSRA
                     EVERARQLPSTRGLTAWCYETSRKPFHLAAAGLLVGLESQVPGIYKRNLPPLRTHYGF
                     TDHEVEFFAIHIDADEVHGERGYQIVERHCPDPDRQAEAVDTVRQATEMRWQYMSGLH
                     RAFVLKDDL"
     gene            complement(1313..1681)
                     /locus_tag="VFX87_15595"
     CDS             complement(1313..1681)
                     /locus_tag="VFX87_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4996408.1"
                     /translation="MDHHAAPETLGVAQVFDLHALKDFAPDRRVRKMLFKTEQLWSEI
                     ACYEPGQSTVMHQHPREEEAIYVLEGCANMSIDGHEVVVPAGALVKFPAAVPHDVRNL
                     GTERCVIMFLKVNPKVLKPA"
     gene            complement(1691..>2505)
                     /locus_tag="VFX87_15600"
     CDS             complement(1691..>2505)
                     /locus_tag="VFX87_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4996409.1"
                     /translation="RCTLRGCGDVCSLACAEALDALIRAEGPDSVSAFIAEPVVGASA
                     GAVVPPPGYYETVREICDRHGVLFIADEVMSGMGRTGRWFGIEHWPGVRPDIVTCGKG
                     VTSGYVPGGAVLVRGELADAVNQAGGFPHGFTYSHHPVVAAVGLAVLHYVEKHGLVAR
                     AEVAGAKLLRRLHGLLDLPAVGEVRGRGLLTAVEIVADRDRRESYPGGDKMAERIQQE
                     AMRRGVMVYASGGQDRGQGDLLLLGPPFTISDAEIEEAVGTLGDAISAVTRA"
BASE COUNT          371 a          855 c          907 g          372 t
ORIGIN      
        1 gaggctcgtt gaagcggtgg gtggggcgcc ggtgcccggc ggcggtgaag gccttggcga
       61 cggcgatctc gaccccggtg agacgggctc ccgtcatccg gtggagcgcg atgggatgcc
      121 cgccgtcgtc acagacgcag atggtctcgg tcacctcgat ctctcgggcc ttggcctcgg
      181 ccgccgcgag cagcaccttg gcgtcctcga gggtgagccg cttcacggtg atcattcggc
      241 gggtacctcc acgaagacgc tatcaccgtc gatgcggacc ggataggacg gcacgcgggc
      301 gccgcgcccc gggaagaggc actggccgct gcggacgtcg aagatccagc cgtgcagcgg
      361 gcaggcgagc cgcatgccgg aaagcttgcc ctcgctgagc catcccccgc ggtggggaca
      421 cacgtcacca caggcgtgca cggcgtcacc cacgcggacg agcacgatgc gggtgccgtc
      481 cacctccacc ggcctgagcg ctcccgcggg cagatccgtg agcgcggcca cccggacttc
      541 ccgggcgccg gtcacgccta gaggtcgtcc ttgaggacaa acgcgcggtg aagcccgctc
      601 atgtactgcc agcgcatctc ggtggcctgg cgcaccgtat ccacggcctc ggcctgccga
      661 tccgggtcgg ggcagtggcg ctccacgatc tgatagccgc gctcgccgtg gacctcgtcg
      721 gcatcgatgt ggatggcgaa gaactccacc tcgtgatcgg tgaagccgta gtgggtgcgg
      781 agcgggggga gattccgctt gtagataccg ggcacctggg actcgagccc caccaggagc
      841 ccggccgcgg ccagatggaa gggctttcgg gacgtctcgt agcaccaggc ggtgagcccg
      901 cgggtgctgg gcagctggcg cgcgcgctcg acctcggcgc ggctcactcc gcacgcctcg
      961 ccgaagcgga tgaggagatc cacgtgcttg gtgccgcttt cctcctcgat gatgttctcg
     1021 agcaggaagt cgcgcgcgtc cgagtcgggg caattgccgt agatggtggc gcaccaccgc
     1081 gcgaactgcg acacgtactg gtagtgctgg cacacccagg cgccgagctg ggcgcgggtc
     1141 agctcgccgt tgacccactt ctcggtgaag gggttgatgc ggctgtggcg ggcattgacg
     1201 gcgtcctcga gctgggtgcg gaaggggctc gcgctgctca tggaggctct cctctcggtc
     1261 actcggtggc ggccacggct ggctcgacga tacgaatccg gccggcctca agtcaagcgg
     1321 gcttgagcac cttggggttc accttgagga acatgatcac gcagcgctcg gtccccaggt
     1381 tgcggacgtc gtgcgggacg gcggcgggga acttgacgag ggcgccggcg ggaacgacca
     1441 cctcgtggcc gtcgatgctc atgttcgcgc acccctcgag gacgtagatc gcctcctcct
     1501 cgcgggggtg ctggtgcatg acggtggact ggcccggctc gtagcaggcg atctccgacc
     1561 agagctgctc ggtcttgaag agcatcttgc gcacgcgccg gtcgggcgcg aagtccttga
     1621 gggcgtggag atcgaagacc tgcgcgacgc cgagggtctc gggagcggcg tggtggtcca
     1681 ttggtctccc tcaggcgcgc gtgaccgccg agatggcgtc gccgagcgtg ccgacggcct
     1741 cctcgatctc cgcgtcgctg atggtgaagg gcggcccgag caggaggaga tcgccctggc
     1801 cgcggtcctg gccgccggag gcatagacca tgacgccccg gcgcatggcc tcctgctgga
     1861 tgcgctcggc catcttgtcc ccgcccgggt acgattcccg gcggtcgcgg tcggcaacga
     1921 tctccaccgc ggtgagcagg ccccgccccc gcacctcgcc caccgcgggg aggtcgagca
     1981 ggccgtggag ccgcctgagg agcttggcgc cggcgacctc cgcgcgcgcc accagaccgt
     2041 gcttctccac gtagtggagc accgcgagtc ctaccgcggc gaccaccgga tgatggctat
     2101 aggtgaagcc gtgcgggaag ccgccggcct ggttcaccgc gtcggccagc tcgccgcgca
     2161 cgagcaccgc gccgcccggg acatagccgc tggtcacgcc cttgccgcac gtgacgatgt
     2221 cgggccggac gccgggccag tgctcgatgc cgaaccaccg gccggtccgc cccatcccgc
     2281 tcatgacctc gtcggcgatg aagagcacgc cgtgacggtc acagatctcg cggaccgtct
     2341 cgtagtagcc gggcggggga accacggcgc ccgcggaagc gcccaccacc ggctccgcga
     2401 tgaaggcgga caccgaatcc gggccctcgg cgcggatgag ggcgtcgagc gcctcggcgc
     2461 aggcgaggga gcagacatcc ccgcagccgc gcagagtgca gcgat
//
LOCUS       DASUFA010000430         5359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1708064, whole genome shotgun sequence.
ACCESSION   DASUFA010000430 DASUFA010000000
VERSION     DASUFA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708064"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="hemC"
                     /locus_tag="VFX87_15605"
     CDS             <1..665
                     /gene="hemC"
                     /locus_tag="VFX87_15605"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX4996410.1"
                     /translation="LPAALILGAFPLREDPRDVLLGRHDGGWDALPAGAVVGTSSPRR
                     RAMVLVRRPDLRAEPIRGNVDTRLEKLRTGAYDALIMAAAGLKRLGLSPAHCTPLDPV
                     EFVPAVGQGVLAVEARQADRELLELLRSVDDTRTRLTALAERAFLARLGAGCHTPVAG
                     HARLEGRTLVLTGVVSSPDGATVLRASVRGSATDGERLGVTLADDLMAKGAKAVLDAS
                     RG"
     gene            673..1470
                     /locus_tag="VFX87_15610"
     CDS             673..1470
                     /locus_tag="VFX87_15610"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX4996411.1"
                     /translation="MAQARGSKPLSGRVVLVTRAVEQAGVFAALLEAAGARVMLVPTI
                     AIEPPDSWAPLDGALAQEYDWVVFTSVNGVAMVRRRVEATGQGRALLERSRLAAIGPA
                     TAAALADWGLVAEVVPGEYVAEGLLDRLRPLVAPGARVLLPRAAETRDVLVRELTAHG
                     ARVTEVAAYRTRAATEHAAGLREALAEGRVDVVTFTSSSTVRSFCALFGSAELPRLLR
                     GVTVACIGPITRATAEGRGLAAQIVPEDYTIPALARAIVTHFESARS"
     gene            1474..2457
                     /gene="hemB"
                     /locus_tag="VFX87_15615"
     CDS             1474..2457
                     /gene="hemB"
                     /locus_tag="VFX87_15615"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX4996412.1"
                     /translation="MPFPVYRPRRLRESPLMRSMVRETSLRIDDFVYPLFAVHGRGVR
                     EPIGSMPGQYRLSIDELLKECKDAASMGIPAVLLFGLPREKDPRGTEAYAEDGIIQQA
                     VRAVKDTIPDLLVITDVCLCEYTSHGHCGVVEDGRIKNDPTLELIARTAVSHAEAGAD
                     LIAPSDMMDGRVAAIREGLDESGFPETPIMAYSAKYASAFYGPFREAADSTPQFGDRR
                     SYQMDPANAMEAMREVALDVDEGADIVMVKPALPYLDVIARVKGEFGLPVAAYSVSGE
                     YAMLKAAGQLGWLDEDRAVLEALTGIRRAGADIIITYFAKDAARLIEQGRA"
     gene            2454..2891
                     /locus_tag="VFX87_15620"
     CDS             2454..2891
                     /locus_tag="VFX87_15620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX4996413.1"
                     /translation="MRISAKGEYAIKAMVDLALHDGQDLQPIQDVASRQGIPQRYLEQ
                     VLLQLKRSGFLYARRGSAGGYRLSRPADQVTVGALLRAVDGPSSAPDSPRRGGRGEDA
                     AGLHELWQEIEEAVASVVDHTTLDDLRRRALDRRRATHPMYHI"
     gene            2924..3868
                     /gene="cysK"
                     /locus_tag="VFX87_15625"
     CDS             2924..3868
                     /gene="cysK"
                     /locus_tag="VFX87_15625"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX4996414.1"
                     /translation="MTTKRATVPSRPKIAGSVLELVGGTPLVRLNRIPKPGGAIVLAK
                     LESLNPGGSVKDRVALAMVEDAERRGVLKAGATLVEPTSGNTGIGLAMVCAVRGYRLI
                     LTMPDDMSAERQRLLARYGAEIHLTPAIEGMTGAVFAAQEICREHPEYYMPMQFENPA
                     NPEAHRRTTGLEILDATGERLDVFVAAVGTGGTVTGVGEVLKERLPGVRVIGVEPARS
                     PVLSGGRFRPHGIQGIGASFVPGILNRSVLDDIIQVRDEDATTTARRLAREEGLLVGI
                     SSGANVFAACQVAETLPADQVVVTVLCDTGERYLSVTF"
     gene            3879..4202
                     /locus_tag="VFX87_15630"
     CDS             3879..4202
                     /locus_tag="VFX87_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX4996415.1"
                     /translation="MSVQVYIPTPFRRATKNADRVAVEAPDVKGLLDELEDQFGGLKG
                     LVRNDQGEVHHHVNIYVNNEAIEALQGLETALKDGDEVSIIPALAGGGPALAGGGPAL
                     AHGRG"
     gene            4213..4674
                     /locus_tag="VFX87_15635"
     CDS             4213..4674
                     /locus_tag="VFX87_15635"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX4996416.1"
                     /translation="MILTPEELARVRAQAEAEYPAECCGVVLARSVPASDRALMPCRN
                     IQDELHAQDPARHPRDSRTAYFIDPKDLLAIGRREAQGYRVATIYHSHVDTGAYFSPT
                     DRQNALINGEPSYPDAVYVVLSVLEGRVADAAAFVWDTSARDFVPTEFPKP"
     gene            4683..>5359
                     /locus_tag="VFX87_15640"
     CDS             4683..>5359
                     /locus_tag="VFX87_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4996417.1"
                     /translation="MPDSEALFRAAQRLMPGGVNSPVRAFRGVGGTPFFVARAEGARL
                     IDVDGRSYVDFLGSWGPLILGHAAAPVVEAVSEAVRRGTSYGASTAGEVEMAELIQRA
                     VPSMEMMRLVSSGTEAAMSALRVARGATGRDFILKFEGCYHGHADSLLVKAGSGGATF
                     GVPDSLGVPSALAALTLTVPFNDLDAVRRQLEAHPGQVAAVIVEPVAGNMGVVPPAPG
                     FLAGLREL"
BASE COUNT          740 a         1892 c         1962 g          765 t
ORIGIN      
        1 agctgccggc cgccctcatc ctgggtgcgt tcccgctccg cgaggacccg cgcgacgtcc
       61 tgctcggtcg gcacgacggg ggatgggacg cgctgcccgc gggcgcggtg gtggggacgt
      121 cgagcccgcg gcgccgcgcg atggtgctcg tgcgccgccc ggatctccgc gccgagccga
      181 tccgcggaaa cgtggacaca cgcctcgaga agctccggac cggcgcctac gatgcgctca
      241 tcatggcggc ggccgggctc aagcgcctgg ggctcagccc ggcgcactgc acgccgctcg
      301 atccggtgga gttcgtgccc gcggtggggc agggagtgct ggccgtcgaa gcccgccaag
      361 ccgaccggga actgcttgaa cttctccgca gcgtggacga cactcgaacg agactcacgg
      421 ccctggcgga gcgggcattc ctggcccggc tgggggcggg gtgccatacg ccggtggccg
      481 gtcacgcgcg tctggagggt cgtaccctgg tgctgaccgg ggtcgtgtcg agcccggacg
      541 gggcgaccgt cctgcgggcc agcgtgagag ggtctgccac ggacggcgag cggctgggcg
      601 tgacgctggc ggatgatctc atggccaagg gggcgaaggc agttctcgat gcgagccgcg
      661 ggtgatcaag tgatggccca ggcgagggga tctaaacccc tgtccggccg cgtggtcctg
      721 gtgaccagag cggtcgagca ggccggcgtg ttcgccgcgc tgctcgaggc cgccggtgcc
      781 cgggtgatgc tcgtgccgac catcgccatc gagccccccg attcctgggc tccactcgac
      841 ggggccctcg cccaggagta cgactgggtg gtgttcacga gcgtcaacgg cgtggccatg
      901 gtgcgccgcc gggtcgaggc caccggccaa ggccgggcgc tgctcgagcg gagccgcctg
      961 gccgccatcg gtcccgcgac ggcggctgcg ctggctgact gggggctggt cgccgaggtg
     1021 gtgcccggag agtacgtcgc cgagggcctg ctcgatcgac ttcggcccct ggtggccccg
     1081 ggagcgcgcg tgctgctgcc tcgcgcggcg gagacccgcg acgtgctcgt gcgcgagctg
     1141 accgcgcacg gggcccgcgt caccgaggtg gccgcctacc gcacgcgcgc ggccaccgag
     1201 cacgcggcgg gactgcgcga ggcgctcgcg gagggccggg tggacgtggt gaccttcacc
     1261 agctcgtcca ccgtgcggag cttctgcgcg ctcttcggct cggccgaatt gccgcggctc
     1321 ctcaggggcg tcaccgtcgc ctgcatcggc cccatcacgc gggccaccgc ggagggccgc
     1381 gggctcgccg cccagatcgt cccggaggac tacacgatcc cggccctggc ccgcgccatc
     1441 gtgacccact tcgagtccgc aaggagctag cgcatgccct ttcccgtcta ccgtccccgc
     1501 cggcttcggg agtcgccgct gatgcgctcc atggtgcgcg agacctcgct gcgcatcgac
     1561 gacttcgtct acccgctctt cgcggtccat ggccgcgggg tccgcgagcc gatcggttcc
     1621 atgccggggc agtaccggct ctccatcgac gagctgctca aggagtgcaa ggacgccgcc
     1681 tccatgggca tcccggcggt gctgctgttc gggctaccgc gcgagaagga tccgcgcggc
     1741 accgaggcct acgcggagga cgggatcatc cagcaggcgg tgcgggcggt gaaggacacg
     1801 atcccggatc tgctggtgat caccgacgtg tgtctctgcg agtacaccag tcacgggcac
     1861 tgcggggtcg tggaggacgg ccggatcaag aacgatccca cgctcgagct catcgcccgc
     1921 accgcggtct cccacgcgga ggccggcgcc gacctgattg cgccgtccga catgatggac
     1981 ggccgggtcg ccgcgatccg ggagggcctc gacgagtcgg gcttcccgga gacgccgatc
     2041 atggcctact cggccaagta cgcctccgcg ttctacggcc ccttccgcga ggccgccgac
     2101 tccaccccgc agttcggcga ccggcgctcc taccagatgg atcccgccaa cgcgatggag
     2161 gcgatgcggg aggttgcgct cgacgtggac gagggcgccg acatcgtgat ggtgaagccg
     2221 gcgctgccct acctggacgt gatcgcccgc gtgaagggcg agttcgggct cccggtggcc
     2281 gcctactcgg tctcgggcga gtacgcgatg ctcaaggcgg ccggccagct cggctggctc
     2341 gacgaggatc gcgcggtgct ggaggcgctg accggcatcc ggcgggccgg ggccgacatc
     2401 atcatcacct acttcgccaa ggacgccgcc cggctcatcg agcagggccg cgcgtgagga
     2461 tctcggcgaa gggcgagtac gccatcaagg cgatggtgga cctcgccctg cacgacggcc
     2521 aggacctgca gcccatccag gacgtcgcat cccgccaggg catcccgcag cgctacctgg
     2581 agcaggtcct gctccagctc aagcgctccg ggttcctcta cgcgcggcgc ggctcggcgg
     2641 gcggctaccg gctgagccgg cccgcggacc aggtcacggt gggcgcgctc ctgcgggcgg
     2701 tggacggccc gtcgtccgcg cccgactcgc cgcgacgggg cggccgcggt gaggacgcgg
     2761 cgggcctgca cgagctgtgg caggagatcg aggaggcggt cgcgtccgtg gtggatcaca
     2821 ccacgctcga cgatctgcgc cggcgcgccc tcgatcggcg gcgcgcgacg cacccgatgt
     2881 accacatcta ggtcttgcaa gagatgccgg acaggacggg accatgacga ccaagcgagc
     2941 gaccgtaccg tcgcggccga agatagcggg ctcggtgctg gagctggtgg gcggcacgcc
     3001 gctggtgcgc ctcaaccgca tcccgaagcc gggcggcgcc atcgtgctgg ccaagctcga
     3061 atcgctcaat ccgggcggga gcgtcaagga ccgcgtcgcg ctcgccatgg tcgaggacgc
     3121 ggagcggcgc ggggtcctca aggcgggcgc gaccctggtg gagcccacca gcggcaatac
     3181 cggcatcggg ctcgccatgg tgtgcgcggt gcggggctat cgcctgatcc tcacgatgcc
     3241 cgacgacatg agcgcggagc ggcagcgcct gctcgcccgc tacggcgccg agatccacct
     3301 gaccccggcc atcgagggca tgaccggcgc cgtcttcgcg gcccaggaga tctgccgcga
     3361 gcatcccgag tactacatgc cgatgcagtt cgagaatccc gcgaaccccg aggcgcaccg
     3421 ccgcaccacc gggctggaga tcctggacgc caccggggag cggctggacg tcttcgtcgc
     3481 ggcggtcggc accggcggca ccgtgaccgg ggtgggcgag gtcctcaagg agcggctacc
     3541 cggtgtgcgc gtgatcggag tggagccggc gcggtcgccc gtgctctccg gcgggcgctt
     3601 ccgaccccac ggcatccagg ggatcggcgc ctcgttcgtg cccgggatcc tgaatcgttc
     3661 cgtgctcgac gacatcatac aggtgcgtga cgaggatgcg acgaccacgg cccggcggct
     3721 cgcccgcgag gagggcctgc tggtgggcat ctcgtccggc gccaacgtgt ttgcggcctg
     3781 ccaggtcgcg gagacactgc ccgccgacca ggtggtggtc acggtgctat gcgatactgg
     3841 ggaacgctat ctgagcgtca ccttctagga gagcggccat gagtgtccag gtttacatcc
     3901 cgacgccgtt ccgccgcgcg accaagaacg cggaccgggt cgccgtcgag gcgcccgacg
     3961 tgaagggcct gctcgacgag ctggaagatc agttcggcgg cttgaagggc ctcgtgcgca
     4021 acgatcaggg tgaggtgcac catcacgtga acatctacgt gaacaacgag gccatcgagg
     4081 cgctgcaggg gctcgagacc gcgctgaagg acggcgacga ggtgtcgatc attccagccc
     4141 tcgcgggcgg cgggccagcc ctcgcgggcg gcgggccggc cctcgcgcac ggtcgagggt
     4201 gatcgcggcg ggatgatcct gacccccgag gagctcgccc gcgtgcgcgc gcaggccgag
     4261 gccgagtatc ccgcggaatg ctgtggcgtg gtgctggccc gctcggtacc cgcgtccgac
     4321 cgggcgctga tgccctgccg caatatccag gacgagctcc acgcgcagga ccccgcgcgg
     4381 cacccgcgcg attcgcggac cgcctacttc atcgacccca aggatctcct ggccatcggc
     4441 cggcgcgagg cgcaggggta tcgggtggcc accatctacc actcccacgt ggacaccggc
     4501 gcgtacttct cgcccaccga ccggcagaac gccctcatca acggtgagcc atcctatccg
     4561 gacgcggtct acgtagtcct gtcggtgctc gagggccgcg tcgccgacgc ggccgccttc
     4621 gtctgggata cctccgcccg cgatttcgtc cccaccgaat tcccgaagcc atgagaggcc
     4681 ccatgcccga ctccgaagcc ctcttcaggg ccgcgcagcg cctgatgccc ggcggcgtca
     4741 acagcccggt gcgcgccttc cgcggcgtgg gcggcacgcc cttcttcgtg gcgcgcgccg
     4801 agggcgcgcg gctcatcgac gtggacggcc gctcctacgt ggacttcctg ggttcgtggg
     4861 ggccgctgat cctgggccac gcggcggctc cggtggtcga ggcggtgagc gaagcggtcc
     4921 ggcgcggcac cagctacggt gcctcgaccg cgggcgaggt ggagatggcc gagctgatcc
     4981 agcgcgcggt gccctccatg gagatgatgc ggctcgtctc ctccggcacc gaggcggcca
     5041 tgagcgcgct gcgcgtggcc cggggcgcca ccggccgcga cttcatcctc aagttcgagg
     5101 gctgctacca cggtcacgcc gacagccttc tcgtcaaggc gggctccggc ggcgcgacgt
     5161 tcggggttcc cgacagcctg ggcgtgccgt ccgcgctcgc ggcgctgact ctcaccgtgc
     5221 cgttcaacga cctggacgcg gtccgccgcc agctcgaggc gcatccgggc caggtcgcag
     5281 cggtgatcgt ggagccggtg gcgggcaata tgggcgtggt gccgcccgcg cccggcttcc
     5341 tcgccgggct gcgcgagct
//
LOCUS       DASUFA010000431         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_1258111, whole genome shotgun sequence.
ACCESSION   DASUFA010000431 DASUFA010000000
VERSION     DASUFA010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258111"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFX87_15645"
     CDS             complement(<1..756)
                     /locus_tag="VFX87_15645"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4996418.1"
                     /translation="MQATAAVLYEVRKPVVVEDVEVMDPGSHEVLVRWAANGVCHSDL
                     HVITGDYPHPLPVVLGHEAAGVVEKIGPGVETVKAGDHVCSSYIPSCGRCSYCIGGQP
                     TMCALRDKPRWFMLDGTSRFRRNGQPLHHFLQVAGYATHAVLPEQSVIPIRADAPLDV
                     VCLVSCGVLAGAGPVFNRAKVPPGASVAVWGAGGVGLNTIQAARMLGAGKIIAVDVMR
                     QKLAWAEEFGATHTVDASREDPVAAVQRMSGTGG"
     gene            885..1997
                     /locus_tag="VFX87_15650"
     CDS             885..1997
                     /locus_tag="VFX87_15650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996419.1"
                     /translation="MNFDFSADQKSLREQARKFLGEHASSTRVRRILEGAAPYDVELW
                     KGMGEMGWMGTAIPETHGGAGFGYLELCVIAEELGRSLAPTPFASTIYLAAEALRLAG
                     TEAQKKRWLPRIAQGEAIGCLALSEGPRVTTPANLATRADGARLSGAKVPVMDGDVAD
                     FAIVLAAQGDEHAGLYLVDLTGPGVTRASLATVDPTRSHARIVLEGAAGEPLGAAGQG
                     WPLVARLLDRAAVLVAFEQVGGAQAALDMAREYALGRFAFGRQIASFQAIKHKLADMY
                     VGIELARSNAYYGAWALSKDAPELGVAAAAARVAASEAYYQAAKENIQVHGGMGFTWE
                     FDCHLHYRRAKLTGLMLGSARRWKDLLVTRLEAGAA"
BASE COUNT          294 a          755 c          711 g          279 t
ORIGIN      
        1 gccacccgtg ccgctcatcc gctgtaccgc cgcgaccgga tcctctcggc tggcatccac
       61 ggtgtgcgtg gcgccgaact cctcggccca ggcgagcttc tggcgcatca cgtccaccgc
      121 gatgatcttg cccgcgccca gcatgcgcgc ggcctggatc gtgttcagcc cgacgccccc
      181 cgcgccccag acggccacgc tggcgcccgg cggcaccttg gcgcggttga acacgggccc
      241 ggcgcccgcg agcacgccgc agctcaccag gcagaccacg tcgagcgggg cgtcggcgcg
      301 gatcgggatc acactctgct cgggcagcac cgcgtgcgtc gcatagcccg cgacctgcag
      361 gaaatgatgg agcggctggc cgttccggcg gaaccgcgag gtaccgtcga gcatgaacca
      421 gcgcggcttg tcgcgcagcg cgcacatggt gggctgtccc ccgatgcagt acgagcaccg
      481 cccgcaggag gggatatagc tcgagcagac gtgatcgccc gccttcacgg tctcgacccc
      541 cggcccgatc ttctccacca ccccggccgc ctcgtggccc agcaccaccg gcagcggatg
      601 gggatagtcc ccggtgatca cgtgcagatc gctgtggcag accccgttgg ccgcccaccg
      661 cacgagcacc tcgtgcgatc ccgggtccat cacctcgacg tcctccacca ccaccggttt
      721 ccggacctcg tagagcaccg ccgccgtcgc ctgcatggcc gcctccgtgt tcttctccct
      781 ctccccttcg gggagaggga agggtgaggg gtgtccgaga gtgaggcccg attctgaaag
      841 agacgcgccc gcatggcaag gggctttggt atgatcctcg cgccatgaac ttcgacttct
      901 cggccgacca gaaatcgctg cgcgagcagg cgcggaagtt cctcggcgag cacgcctcgt
      961 cgacccgcgt ccgccgcatc ctggaaggcg ccgcgcccta cgacgtggag ctctggaagg
     1021 ggatgggcga gatgggctgg atgggcaccg ccatccccga gacccacggc ggcgccggct
     1081 tcggctacct cgagctctgc gtgatcgcgg aggagctggg ccggagcctg gcccccacgc
     1141 cgttcgcctc cacgatctac ctggccgcgg aggccctgcg cctcgcgggc accgaggcgc
     1201 agaagaagcg ctggctgccg cgcatcgcgc agggcgaggc gatcggctgc ctcgcgctat
     1261 ccgagggccc acgggtgacg acgccggcca acctggcgac gcgcgccgac ggcgcccgcc
     1321 tgagcggcgc caaggtcccg gtgatggacg gcgacgtggc cgacttcgcg atcgtcctcg
     1381 ccgcgcaggg cgatgagcat gccggcctct acctggtcga cctcaccggc cccggcgtca
     1441 cgcgcgcgag cctcgccacc gtggacccga cccgctcgca cgcgcggatc gtgctcgagg
     1501 gcgcggcggg cgagcccctg ggggcggccg gccagggctg gccgctggta gcgcggctgc
     1561 tcgatcgcgc ggcggtgctg gtggccttcg agcaggtcgg cggggcgcag gccgcgctcg
     1621 acatggcgcg cgagtacgcc ctcggccgct tcgccttcgg gcggcagatc gcctcgttcc
     1681 aggccatcaa gcacaagctc gccgacatgt acgtgggcat cgagctggcg cggtcgaacg
     1741 cctactacgg cgcctgggcg ctctcgaagg acgcccccga gctgggggtc gcggcggcag
     1801 cggcacgggt ggccgcgagc gaggcctact accaggcggc caaggagaac atccaggtgc
     1861 acggcggcat gggtttcacc tgggagttcg actgccacct ccactaccgg cgcgccaagc
     1921 tcaccggcct catgctgggc agcgcccgtc gctggaagga tctgctcgtc acccggctcg
     1981 aggccggggc ggcctaggag cgcaccgtgg acttcgacga cacccgggaa gaagcggcg
//
LOCUS       DASUFA010000432        13426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_668213, whole genome shotgun sequence.
ACCESSION   DASUFA010000432 DASUFA010000000
VERSION     DASUFA010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668213"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1496
                     /locus_tag="VFX87_15655"
     CDS             156..1496
                     /locus_tag="VFX87_15655"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX4996420.1"
                     /translation="MARRPAATAEGVVVAAVTPRTPAARAGLRPGDRILAINGAALRD
                     VIDFHFHAGDQHLRLSIEREGRARTARLRRAGSGLGLELEAPRPAEIATCANKCVFCF
                     IHQLPKGMRKSLYVKDDDFRLSFLHGNYVTLTDLEEAELARIETQRLSPLYVSVHATD
                     PDLRHALLGRPRVRRELLPVMERLAKAGIAMHAQIVLCPDWNDGAHLDRTVHELARLH
                     PAVSTAAVVPVGLTRHRERLPDLRAVTPEEAGALVRAIEAWQREFRDTLGTRFVWAAD
                     ELYLQAGLPLPGAREYEGFAVAEDGIGLVRRFEDAFARAVGRRPARLPRPRHVTVVTG
                     EMFGPRMRALLDRVVVDGLRIGVVPVANEFFGRGIGVAGLLTGRDIQIQLEQARAAGR
                     ELGDEVLVPAVAIRDGAGVFLDDLTPADLTASLGTPVTTVEPDAPALLAAVLGR"
     gene            1531..2880
                     /gene="der"
                     /locus_tag="VFX87_15660"
     CDS             1531..2880
                     /gene="der"
                     /locus_tag="VFX87_15660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX4996421.1"
                     /translation="MLRPIIAIVGRPNVGKSTLFNRLIGRRRSLVRDVPGVTRDRLYG
                     TAVFERWQATVVDTGGFDPSSASDLVQGVRRQVLQAVDEADLLVFVVDGRAGITGLDE
                     EIAGILRKSGRPTLLAVNKVDSAGQDTPVGDAYRLGFAPVLTVSAEHGRGVAELLEAC
                     RERAPAIAAETAPEGTRVAIIGRPNVGKSSLVNAVLGHERVLVHDLPGTTRDAVDTPV
                     TFRDRNYLLIDTAGIRRKGKVTEALEKLAVVMALKSLERCQVAVLVMDASEGVTAQDA
                     HIAGYANDAGRAVVVAVNKWDLVPSGMVQRADVTGQIHERLPFLEHAPICFTSAVTRL
                     GLRDLFDQVDLVAAEAQKRVQPGDLLATLRTAVERRPMSARGIPLRVQSAQQVAVAPP
                     TFAIRVNLPDSIHFSYERYIINSLRHAYSFAGSPLRLLFRKAVGRGAGRSRGARRRA"
     gene            2996..3727
                     /locus_tag="VFX87_15665"
     CDS             2996..3727
                     /locus_tag="VFX87_15665"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4996422.1"
                     /translation="MSPERPDQDDFLEDEEQEEYGQRSIFATGWFRAVLVLLVLAVIA
                     VIAVPLIGGWFEPTPPPKVTTKPSEPSSTPVPVPSTPPPASAPPVTPPPAAAPTPAPT
                     PTPAVPPPSMPAPAVSSPTPAPARPETPPASASRTTKAAEKAKVPERTAAVTPSKSTA
                     KPAASSGNYWVQVGAFKESGNAEGLARKLRSEGYSVQVTRVTRDAPLHVVRVGGYPDR
                     SRASAAREELQGKGQTGFVTSGPAK"
     gene            3751..4023
                     /locus_tag="VFX87_15670"
     CDS             3751..4023
                     /locus_tag="VFX87_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX4996423.1"
                     /translation="MTKNDLINAVAAHGLSKRQSAAVVESLFDIIFRCFEKGEDVKIV
                     GFGHFRIRQKASRRGRNPQTGDSIEITARKVLTFKPSKGLKLRINV"
     gene            4042..4413
                     /locus_tag="VFX87_15675"
     CDS             4042..4413
                     /locus_tag="VFX87_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4996424.1"
                     /translation="MSTVPDKLYYRIGEVETITEIPAYVLRYWESEFKLLRPKKNPAG
                     QRLYRKADLELVLRIKSLLYEERLTLEGAKKRLRGEARGLDQMDLGLRGATLEEVIRR
                     TRTRLQAIRQRLAASRPGRSS"
     gene            4450..4526
                     /locus_tag="VFX87_15680"
     tRNA            4450..4526
                     /locus_tag="VFX87_15680"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4484..4486,aa:Pro,seq:ggg)
     gene            4539..5312
                     /gene="surE"
                     /locus_tag="VFX87_15685"
     CDS             4539..5312
                     /gene="surE"
                     /locus_tag="VFX87_15685"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX4996425.1"
                     /translation="MAPVILVTNDDGVHARGLQVLADALAALGEVYVVAPDREQSAVG
                     HALTLHRPLRVDRVAERKYAVNGTPSDCVNLAVLGLLPEPPVLVASGVNHGTNLGDDV
                     TYSGTVSAAMEGTLLGVPSLAVSQADPDAEGFDGAGPVALAVATRLLVEGLPAKTLLN
                     VNVPRGAVKGIRLTRLGHRVYREKVVQEMDPRGRPYYWIGAGPPEWAEDPASDIAAVH
                     AGFASVTPLHLDLTHHGALGRMSEWEGALNAVLKKKRKR"
     gene            5309..5959
                     /locus_tag="VFX87_15690"
     CDS             5309..5959
                     /locus_tag="VFX87_15690"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX4996426.1"
                     /translation="MSTQRFAHERERMVDEQVVGRGVTDARVLEVMRRLPRHVFVDEA
                     LRGRAYGDHSLPIGEGQTISQPFIVGRMTELLRLGGREKVLEVGTGCGYQAAVLAELA
                     ARVCTMERLPRLAARARETLEGLGYRNVWVRAGNGTLGWPDEAPFDRILVAAGGPAVP
                     PPLFEQLAEGGRMVMPVGDVASQTLEVIDKVQGVMRVTRDSGCVFVKLVGKYAWES"
     gene            5984..6060
                     /locus_tag="VFX87_15695"
     tRNA            5984..6060
                     /locus_tag="VFX87_15695"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6018..6020,aa:Pro,seq:cgg)
     gene            6091..6396
                     /locus_tag="VFX87_15700"
     CDS             6091..6396
                     /locus_tag="VFX87_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX4996427.1"
                     /translation="MGVTGLRSAAEIVVEGRVQGVGYRDFVQRKAASLGLAGYVMNLK
                     DGKVRVRVEGGRDLIEELMHDLGKGPPLARVDHLAVTWRPLTGRYAGFSIRYAEFDP"
     gene            6393..7319
                     /locus_tag="VFX87_15705"
     CDS             6393..7319
                     /locus_tag="VFX87_15705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4996428.1"
                     /translation="MSRGLRIVVLLVAILLVSTAVSHAQPKPAKARAATSQQQTVPAP
                     HWGSAKAPGTKTAAAAPKTAPKPGMCTHTVRNGESISRVAARYRVTRAALIQANRLVS
                     PHALRPGQRLSVPGCQPPVPQTTAGETPGTEPDADGILIKRVGPRRILTSLVLGTPDF
                     REERITLVWPVQGPVISTFGRRSRGWHAGIDITAEVGSQIYAAAPGTVVYSGWIRAYG
                     QVVKIEHSNGFITLYAHNQNNLVEVGEDVEAGQLIATVGRSGHVTGPHVHFEVRRDGK
                     AYDPLHLLEPSDQSPVFDDDIAASSSEHDPHE"
     gene            7328..8278
                     /locus_tag="VFX87_15710"
     CDS             7328..8278
                     /locus_tag="VFX87_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4996429.1"
                     /translation="MAPEPETTDELEDGDEPLEALLDKNPAELGEKPEAPEAGDRSRE
                     LIALYLQEISRVKLLTPEEEQDLARRVQAGDAEAQKRLVEANLRLVVRIARRYLHRGL
                     SLLDLIEEGNVGLLHAARKFQPDRGTRFSTYATWWIRQAVVRALANQARTIRLPVHVE
                     LLLSQYMKQRNVLTQKLGRPPTLEEVAAVMDRPVGELEQLESLRQQPVSLDKPTGEDG
                     KGNLGETVEDPNAAAGSGLAAMLRARADLAGVLQDLPDRDRTVVTLRFGLGGEEPMTL
                     ERIGQRLGLTRERVRQIEGTALQHLRRLLAARDVEPSDLL"
     gene            8275..8493
                     /locus_tag="VFX87_15715"
     CDS             8275..8493
                     /locus_tag="VFX87_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4996430.1"
                     /translation="MSPGEKSLGERVRELRERQGLTQAQLAERAQLALSYITLAESGQ
                     KVNLSPSALGRLARALGVPSKVLSEVAN"
     gene            complement(8526..9353)
                     /locus_tag="VFX87_15720"
     CDS             complement(8526..9353)
                     /locus_tag="VFX87_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4996431.1"
                     /translation="MSPVDPGPHVSLERMNRFQEAEARRIIGELGLPAGSRGLDVGCG
                     IGLYALWLAEAVGQGGRVVAIEPEADKVEAARALVGGEAAGERLEFQRGDGTAIPLPD
                     RSVDWVWCGDVLHHIVDTEKALREFLRVVRPGGRIIVKESQVFSAMFLPGHPEVERRI
                     QLAEIRRTLDEGAGHSFQERRQTTPASLRAAGLASVAMRTYTLERRAPLAPADHDYIQ
                     HTVFSRNWGDRLRELLTPEDWRERSRLCDEGSPANVLRSPDYYCLYPITVFSARRPA"
     gene            9902..10876
                     /locus_tag="VFX87_15725"
     CDS             9902..10876
                     /locus_tag="VFX87_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996432.1"
                     /translation="MNRRAFLSTLSGGLLAAPLAVEAQQAGKVPRIGVLSSAPGATWD
                     GFRQGLRELGYSEGRTILIEWRWTEGKAERAPELAADLVRLQPEIVTGSPQPTAAGKA
                     ATATIPIVFIAVADPVRVGLVATLARPGGNITGLATLVGAGFVGKLIEVLKEALPQVS
                     RVALLINPTNAMHQEIVANELPEAAERLRLTLLTVEARAADALEGAFDTAVRARAGAI
                     IPLGDPLVFVHRARIAELAAKHRLPAMYLFRESVEAGGLMAYGPSFHDLGRRGASYVD
                     KILKGAKPGDLPVEQPTKFELIINLKTAKALGMTIPPSLLQRADQVIE"
     gene            10876..11862
                     /locus_tag="VFX87_15730"
     CDS             10876..11862
                     /locus_tag="VFX87_15730"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996433.1"
                     /translation="MDRRVFLGTLAGGLLAAPLAAEGQQPARMVKIGYLEFGSAAPGT
                     PRFEAFRRGLRELGWIEGKSIAIVGRAAEGNENRLPEFTVDLVKLKVDLIFASSTPAA
                     LAAKQVATTTPIVIGFVADPVGSGLVASLARPGGNITGWTHLAGLELNAKRLEILAQA
                     VPGAVRIGALWNQANPIHGPGLKEVEAVARRLTVELHAVGVRDPKELEGAFSTLAQQR
                     VQALTVPPDGMFLAHQSRIIDLAAKHRIPTMYGVTELTKAGGLMAYGTNLLDMYRRGA
                     LFVDKILKGAKPGDLPVEQPTKFDLVINLKTAKALGLTIPQSVLQRADQVIE"
     gene            12032..12256
                     /locus_tag="VFX87_15735"
     CDS             12032..12256
                     /locus_tag="VFX87_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996434.1"
                     /translation="MLHLVLTVVGIAQGVVYVGLALLLVAFIWWVFAVQRNPDRTFYA
                     ATRAVLLLALLAALVLGAVPFIRWMRQLTS"
     gene            12855..12931
                     /locus_tag="VFX87_15740"
     tRNA            12855..12931
                     /locus_tag="VFX87_15740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12889..12891,aa:Arg,seq:cct)
BASE COUNT         2089 a         4666 c         4741 g         1930 t
ORIGIN      
        1 catggccgcc gtctacttcc agctcctgcc cgcgttcgtc accacgctcc tggcctgggt
       61 gacggccgcg ttcacgatca cctcggggct gcagtacatc atccgcggac tcaagcagct
      121 cgaggcgtcc acctcggtga gcgggcgcgt ctgacatggc ccgccggccg gccgcgacgg
      181 cagagggcgt ggtggtggcc gcggtgacac cgcggacgcc ggcggcccgg gccggcctgc
      241 gccccgggga ccgcatcctc gcgatcaacg gcgccgcgct gcgcgacgtg atcgacttcc
      301 acttccacgc gggcgaccag cacctgcgcc tctcgatcga gcgggagggc cgggcgcgta
      361 ccgcgcgcct tcgccgcgcc ggctcggggc tcgggctcga gctcgaggct ccgcgccccg
      421 ccgagatcgc cacctgcgcc aacaagtgcg tgttctgctt catccaccag ctgccgaagg
      481 ggatgcgcaa gagcctttac gtgaaggacg acgacttccg gctctcgttc ctgcacggca
      541 actacgtcac gctgacggat ctggaggagg ccgagctcgc gcgcatcgag acgcagcgcc
      601 tctcgccgct ctacgtctcg gtccacgcca ccgacccgga cctgcgccac gccctcctgg
      661 ggcggccgcg ggtacgccgc gagctgctgc cggtgatgga gcggctcgcc aaggcgggca
      721 tcgccatgca cgcgcagatc gtgctgtgcc cggactggaa cgacggcgcg cacctcgacc
      781 gcaccgtgca cgagctggcc cggctccatc ccgcggtgtc gaccgccgcg gtggtgccgg
      841 tcgggctcac gcgccaccgc gagcggctcc cggatctgcg ggcggtgact ccggaagagg
      901 caggcgcgct cgtgcgcgcc atcgaagcct ggcagcgcga gttccgcgac acgctcggca
      961 cgcgcttcgt ctgggccgcg gacgagctgt acctccaggc gggcctcccg ctgcccggcg
     1021 cgcgggagta cgagggcttc gcggtggccg aggacggcat cgggctcgtg cgccgcttcg
     1081 aggacgcctt cgcccgcgcg gtgggccgcc ggcccgcgcg cctgccgcgc ccgcggcacg
     1141 tgaccgtggt gaccggcgag atgttcggtc cgcggatgcg ggcgctgctg gatcgggtcg
     1201 tggtggacgg cctgcgcatc ggcgtcgtgc ccgtggccaa cgagttcttc gggcgcggca
     1261 tcggcgtggc cgggctgctc accggccgcg acatccagat ccagctcgag caggcgcggg
     1321 ccgccggccg cgagctcggc gacgaggtgc tggtaccggc ggtggcgatc cgcgacgggg
     1381 caggggtctt cctcgacgac ctcacgccgg ccgacctgac cgcctcgctc ggcacgcccg
     1441 tcaccaccgt cgaacccgac gccccggccc tcctggccgc cgtccttggc cggtaggggc
     1501 cggtaggggc cgggggggcc gacgctgccc atgctgcgcc cgatcatcgc catcgtgggc
     1561 cgcccgaacg tgggcaagtc cacgctcttc aaccggctca tcggccgccg gcgctcgctc
     1621 gtgcgcgacg tgcccggcgt cacgcgcgac cgcctctacg gcaccgcggt gttcgagcgc
     1681 tggcaggcca ccgtcgtgga caccggcggc ttcgacccga gcagcgcgtc cgacctggtg
     1741 cagggcgtgc gccggcaggt gctgcaggcg gtggacgagg ccgacctgct cgtcttcgtg
     1801 gtggacggcc gcgccggcat caccggcctc gacgaggaga tcgcggggat cctgcgcaag
     1861 tccgggcggc cgacgctgct cgcggtcaac aaggtggata gcgcgggcca ggacaccccg
     1921 gtgggcgacg cctaccggct gggcttcgcg ccggtgctta ccgtctccgc cgagcacggg
     1981 cgcggcgtgg ccgagctcct cgaggcctgc cgggagcgcg cgcccgccat cgcggccgag
     2041 accgcgcccg agggtacccg cgtggcgatc atcgggcgcc ccaacgtcgg caagtcgtcc
     2101 ttggtcaacg cggtgctcgg gcacgagcgt gtgctggtac acgacctacc cggcaccacg
     2161 cgcgacgcgg tggatacgcc ggtgaccttc cgggaccgca actacctgct catcgacacc
     2221 gcgggcatcc gccgcaaggg caaggtgacc gaggccctcg agaagctcgc ggtggtgatg
     2281 gcgctcaaga gcctcgagcg gtgccaggtg gcggtcctgg tcatggacgc ctccgagggc
     2341 gtgaccgcgc aggacgcgca catcgcgggt tacgccaacg acgcgggccg cgcggtggtg
     2401 gtggcggtga acaagtggga cctcgtgccg tccggcatgg tgcagcgggc cgacgtcacc
     2461 gggcagatcc acgagcggct gcccttcctc gagcacgcgc ccatctgctt cacctccgcg
     2521 gtgacgcggc tcggactccg cgatctcttc gaccaggtgg acctggtggc cgcggaggcg
     2581 cagaagcgcg tgcagccggg cgacctgctg gccacgctgc gcaccgcggt cgagcggcgg
     2641 ccgatgtcgg cccgcggcat cccgctgcgg gtgcagtcgg cgcagcaggt ggcggtggcg
     2701 ccgcccacct tcgccatccg cgtgaacctg ccggacagca tccacttctc ctacgagcgc
     2761 tacatcatca actctctgcg gcacgcctac agcttcgcgg gctcgccgct gcgcctgctc
     2821 ttccgcaagg ccgtggggcg gggagcgggc cggtccagag gggcacgccg ccgcgcctga
     2881 gcaggcagcg gctaactagg cagaacccct agtgatttcg gttgagcccc cccggcactg
     2941 gtgctataac atcccctagc cgttcaccat cgagcagtca gcgaggactt agcgtgtgag
     3001 cccagagcgc cccgaccagg acgatttcct agaagacgaa gagcaggaag aatacggcca
     3061 gcgcagcatc ttcgcgaccg gctggttccg cgccgtgctc gttcttctcg tcctcgccgt
     3121 gatcgccgtc atcgcggttc cgctgatcgg tggctggttc gagccgacgc cgccaccgaa
     3181 ggtgaccacg aagccgagcg agccgtcctc gacgcccgtg cccgtgccga gcacgccgcc
     3241 cccggcgagc gcgccgcctg tcacgccgcc gcccgcggcc gctccgacgc ctgctccgac
     3301 acccacgccg gccgtgccgc caccgtcgat gccggcgccc gcggtcagca gcccgacgcc
     3361 cgcgcccgcg cgccccgaga caccgcccgc gagcgcgtcc aggacgacca aggccgcgga
     3421 gaaggccaag gtgcccgagc gcaccgcggc ggtgaccccg agcaagagca cggcaaagcc
     3481 ggcggcttcg agcggtaact actgggtgca ggtgggcgcg ttcaaggaga gtggcaacgc
     3541 cgaaggcctg gcccggaagc tgcgctccga aggctactcg gtgcaggtga cgcgggtgac
     3601 gcgcgacgcg ccgctgcacg tcgtgcgggt cggcggctat ccggatcgct cgcgggccag
     3661 cgcggcccgc gaagagctgc agggcaaggg acagacgggc ttcgtgacct cgggcccggc
     3721 caagtaaacc gacgagggga ggtgacggcg ctgaccaaga acgatctgat caatgcggtg
     3781 gcggctcacg ggctgtcgaa gcggcagtcg gcggctgtgg tcgaatcact gttcgatatc
     3841 atctttcgct gcttcgaaaa gggagaggac gtgaagatcg tgggcttcgg ccatttccgg
     3901 atccggcaga aggcgtcgcg ccgcggccgc aatccccaga cgggcgacag catcgagatc
     3961 accgcgcgca aggtgctgac gttcaagccg agcaaggggc tgaagctccg gatcaacgtc
     4021 tagccggagg cccgcggggg tatgagcacg gttcctgaca agctctacta ccggatcgga
     4081 gaagtcgaga cgatcacgga gatccccgca tacgtgctcc gctactggga gtccgagttc
     4141 aagctgctcc ggccgaagaa gaaccccgcc ggccagcgcc tgtaccgcaa ggccgacctc
     4201 gagctggtcc tccgcatcaa gtcactgctc tacgaggagc gcctcaccct cgaaggcgcc
     4261 aagaaacggc tccgcggcga ggcccgcggc ctcgaccaga tggacctggg gctgcgcggc
     4321 gccacgctcg aagaggtgat ccgccgcacg cgcacgcgcc tgcaggccat ccgccagcgg
     4381 ctcgccgcct cccgacccgg ccgctcgtct tgagtttgcc cgatccagcc cgtacaatgc
     4441 ccgttgggtc ggggcgtggc gcagcccggt agcgcacttg actgggggtc aaggggtcgc
     4501 tggttcaaat ccagtcgccc cgaccaattt caatcgacat ggcgcccgtg attcttgtca
     4561 ccaacgatga cggggttcac gcgcgcgggc tccaggttct ggcagacgct ctggccgcgc
     4621 tcggcgaggt atacgtcgtc gcgccggatc gcgaacagag cgcggtgggt cacgccctca
     4681 cgctgcaccg gccgctccgg gtagaccggg tcgcggagcg gaagtacgcg gtgaatggca
     4741 cgccgtcgga ctgcgtgaac ctggccgtcc tcgggctctt gccggagccg ccggtgctgg
     4801 tggcgtcggg cgtgaaccac gggacgaacc tgggcgacga cgtgacgtac tcgggcacgg
     4861 tgtcggcggc gatggagggc acgctgctgg gggtgccgtc gctggcggtg tcgcaggcgg
     4921 acccggacgc ggagggattc gacggggcgg ggccggtggc gctcgcggtg gccacgcggc
     4981 tcctggtgga ggggctgccc gcgaagacgt tactgaacgt gaacgtgccg cggggcgcgg
     5041 tgaaggggat ccggctcacg cggctcggcc accgggtcta ccgcgagaag gtggtgcagg
     5101 agatggatcc gcgcgggcgc ccgtactact ggatcggggc gggcccgccg gagtgggcgg
     5161 aggacccggc ctcggacatc gcggcggtgc acgcggggtt cgcgtcggtg acaccgctgc
     5221 acctggacct gacgcaccac ggcgccctgg gccgcatgag cgagtgggaa ggcgccctga
     5281 acgccgtgct gaagaagaag cggaagcggt gagcacgcag cgcttcgcgc acgagcgcga
     5341 gcgcatggtg gacgagcagg tcgtcggccg cggagtcacc gacgcgcggg tgctcgaggt
     5401 gatgcggcgg ctgccgcgcc acgtgttcgt ggacgaggcg ctgcgcggcc gggcctacgg
     5461 ggaccactcg ctgccgatcg gggaagggca gacgatctcg cagcccttca tcgtgggccg
     5521 catgaccgag ctgctgcggc tcggcgggcg cgagaaggtg ctggaggtgg gtacggggtg
     5581 cggctaccag gccgcggtgc tcgcggagct cgcggcacgg gtgtgcacga tggagcgcct
     5641 gccgcggctc gccgcgcggg cgcgcgagac gctggaaggg ctcgggtacc gcaacgtgtg
     5701 ggtgcgggcg ggcaacggga cgctcggatg gccggacgag gcgccattcg atcgcatcct
     5761 ggtcgcggcg ggtgggccgg cggtgccgcc gcccctgttc gagcagctgg cggaaggcgg
     5821 gcggatggtg atgccggtgg gcgatgtcgc cagccagacg ctcgaggtga tcgacaaggt
     5881 gcagggggtg atgcgggtca cccgggattc cgggtgcgta ttcgtgaagc tggtcggcaa
     5941 atatgcctgg gagtcgtgag atcctgggtg tgaggtttac ggacggggcg tagcgcagct
     6001 tggtagcgcg cctgcttcgg gagcaggagg tcgctggttc aaatccagtc gccccgacca
     6061 ttttcttctc gagccgttgc cggagccggg atgggcgtca cgggactgcg cagcgccgcc
     6121 gagatcgtgg tcgaaggccg ggtgcagggc gtcggctacc gcgatttcgt ccagcgcaag
     6181 gccgcatcac tgggcctcgc cgggtacgtg atgaatctga aggatggaaa ggtgcgcgtg
     6241 cgcgtggaag gcgggcgcga cctcatcgag gagctcatgc acgacctggg caagggcccg
     6301 ccgctggcgc gggtggatca cctggccgtc acctggcggc cgctgacggg acgctacgcg
     6361 gggttctcca tccgctacgc ggagttcgat ccgtgagccg cggactccgc atcgtcgtcc
     6421 tcctggtcgc catcctcctg gtctccaccg ccgtgtctca cgcacagccc aagcccgcga
     6481 aggcccgggc cgccacctcg cagcagcaaa ccgtcccggc cccgcattgg ggctcggcga
     6541 aggcccccgg cacgaagacc gcggccgccg cccccaagac cgcgccgaag ccggggatgt
     6601 gcacgcacac cgtgcggaac ggggagtcca tcagtcgcgt cgcggcccgg tatcgcgtga
     6661 cccgcgcggc cctgatccag gccaaccgac tcgtgagccc ccatgccctc cggccgggcc
     6721 agcgcctgtc cgtgccgggc tgccagccgc ccgtccccca gaccaccgcc ggggaaaccc
     6781 ccgggaccga gcccgatgcc gacggcatcc tgatcaagcg ggtggggcca cggcgcatcc
     6841 tgacgtccct ggtgctcggc acacccgatt tccgggagga gcgcatcacg ctggtctggc
     6901 ccgtgcaagg acccgtgatc tccaccttcg gccggcgctc ccgagggtgg cacgccggca
     6961 tcgacatcac tgccgaggtg gggtcgcaga tctacgccgc cgcgcccggg acggtcgtct
     7021 acagcggttg gatccgcgcc tacgggcagg tggtgaagat cgagcacagt aacggattca
     7081 tcacccttta cgcccacaac cagaacaacc tggtcgaggt cggggaggac gtcgaggccg
     7141 ggcagctcat cgcgacggtg gggcgcagcg gacatgtcac ggggccccac gtgcacttcg
     7201 aggtgcggcg cgacggcaag gcctacgacc ccctccacct gctcgagcct tccgatcaga
     7261 gcccggtctt cgatgacgac atcgccgcat ccagctcgga acacgaccct cacgagtgag
     7321 ggtccccatg gctccggagc ccgagacgac ggatgagctg gaggacggcg acgagcccct
     7381 cgaagccctg ctggacaaga accccgccga gctcggcgag aagcccgaag cccccgaggc
     7441 cggcgaccgc agccgcgagc tgatcgcgct ctacctgcag gagatctccc gggtcaagct
     7501 gctgaccccg gaggaggagc aggacctggc ccgccgcgtc caggccggcg acgccgaggc
     7561 ccagaagcgg ctcgtcgagg ccaacctgcg gctcgtggtc cggatcgccc gccgctatct
     7621 ccaccgcggg ctctcgctcc tcgatctgat cgaggagggc aatgtcgggc tgctgcacgc
     7681 cgcacgcaag tttcagcccg atcgcggcac gcgcttctcg acctacgcca cctggtggat
     7741 ccgccaggcg gtggtgcgcg cgctcgccaa ccaggcgcgg acgatccggc tgccggtcca
     7801 cgtggagctg ctcctctcgc agtacatgaa gcagcgcaac gtgctcaccc agaagcttgg
     7861 ccggccgccc acgctcgagg aggtggcggc ggtgatggac cggcccgtcg gcgagctgga
     7921 acagctcgag agccttcgcc agcagccggt ctcgctcgac aagcccaccg gggaggacgg
     7981 caagggaaat ctcggcgaga cggtcgagga cccgaacgcg gcggcgggca gcgggctcgc
     8041 cgccatgctg cgcgcccggg ccgatctggc cggcgtgctc caggatctgc ccgaccggga
     8101 tcgcaccgtc gtgaccctgc gcttcggcct gggtggcgag gagcccatga cgctcgagcg
     8161 catcggccag cgcctgggtc tcacccgcga gcgcgtgcgc caaatcgagg gcaccgcgct
     8221 ccagcacctg cggcgcctgc tcgccgcgcg cgacgtcgag ccgtcggatc tgctgtgagc
     8281 ccgggcgaga agagcctggg cgagcgggtg cgggagctgc gcgagcgcca gggcctcacc
     8341 caggcgcagc tcgccgagcg ggcccagctc gcgctcagct acatcacgct ggcggagtcg
     8401 ggccagaagg tgaacctgtc gccgtcggcg ctcggccggc tcgcgcgcgc cctgggcgtg
     8461 ccgagcaagg tgctgagcga ggtggccaac taggcggcgc ggtcgacgcg ctcggccctg
     8521 aagcgtcagg cgggccggcg ggccgagaag accgtgatcg ggtagaggca gtagtagtcg
     8581 gggctccgca gcacgttggc cggcgagccc tcgtcgcaga gccggctgcg ctcccgccag
     8641 tcctccggcg tcagcagctc ccgcaggcga tcgccccagt tgcgcgagaa gacggtgtgc
     8701 tggatgtagt cgtgatcggc cggagccagc ggcgcacggc gctccagcgt gtaggtgcgc
     8761 atcgccacgc tcgcgaggcc ggccgcacgg agggacgcgg gcgtggtctg ccgccgctcc
     8821 tggaaggagt gccccgcgcc ttcgtccagc gtgcggcgga tctcggcaag ctggatgcgc
     8881 cgctcgacct ccgggtggcc gggcaggaac atggccgaga agacctgcga ctccttgacg
     8941 atgatgcggc cgcccggccg gaccacccgg aggaactccc gcaaggcctt ctccgtgtcc
     9001 acgatgtggt gcagcacgtc cccacaccac acccagtcca cgctgcggtc gggcagggga
     9061 atggccgtac cgtcgccccg ctggaactcg agccgctcgc cggcggcctc accgcccacg
     9121 agcgcgcgcg ccgcctccac cttgtccgcc tcgggctcga tggccacgac ccggccgccc
     9181 tggcccaccg cctcggccag ccagagcgcg tagaggccga tgccgcagcc cacgtcgagc
     9241 ccccgcgagc cggcgggcag ccccagctcg ccgatgattc gccgcgcttc cgcctcttgg
     9301 aagcggttca tgcgctcgag cgagacgtgg ggacccggat cgacagggct catcgcgcgc
     9361 catgctacca gacggccgct cgcgatgggg tgtagaatcg agaccgcacc cagcgaatca
     9421 cgcgagcagc agctccacga cttacctcgc gtgagcgcat cacgccgatg cacaaccccc
     9481 gggcctcggc agccagaagt ggacatactc cgcgccctcg acggcacatg gattgcggcc
     9541 gagctacccg cgctgatcga ggccaccgac cgcatcgccg cgcagtggac gcgcttcgcg
     9601 gaggccaacc ggcgccctcc cgggcggggc gagccggcct ggagcctagc gcaccatgtc
     9661 ggacacgccc aaggcacccg tagctggctc tagccccgaa cgttcggctc ctgtaggctc
     9721 cccgcagccg gtctgcgtgg tctgtggcgg gcctcgggac cctaggaagc gggaggcgtg
     9781 ctcggacaag tgccgagcgg ccctgagtcg gcggcggcgc gaggatactc aacggacccg
     9841 ggacgaagag ggtccgagcc ctcttggaaa ccgcaccgaa gaagctgggg agagagcggc
     9901 catgaatcgt cgagcgttcc ttagcaccct gagcggtggc ctcctcgccg cgccgctcgc
     9961 cgtcgaggcg cagcaggcgg ggaaggttcc gcgaatcgga gtgctcagca gcgcgcccgg
    10021 cgcgacgtgg gacgggttcc gccagggact tcgcgagctg ggatactcgg agggccgaac
    10081 cattctcatc gagtggcgat ggacggaggg gaaagccgag cgcgccccgg agctcgctgc
    10141 cgatctggtc cgcctccagc ccgagatcgt gaccgggtcg ccccagccaa ccgccgcggg
    10201 caaggcggcc acagcgacga ttcccatcgt gtttatcgcg gtggctgatc ccgtcagagt
    10261 ggggctcgtc gccactcttg cccgaccggg tggcaacatc accggcctcg ccacgctggt
    10321 gggggcggga ttcgtgggga aactgatcga ggtgctcaag gaggcccttc cccaggtctc
    10381 gcgggtggcc ttgctgatca atcctaccaa tgcgatgcac caagagatcg tggccaacga
    10441 gcttcccgag gcggctgaac ggctgcggct gaccctcctc accgtagagg cacgggcagc
    10501 cgacgccctc gagggcgcgt tcgacaccgc ggtccgcgcg cgggccgggg caatcattcc
    10561 gttgggggat cctctcgtct tcgtccaccg ggcacggatc gctgaacttg cagcgaaaca
    10621 ccgtctgccc gcgatgtatc ttttccgcga gagcgtcgag gccggaggcc tcatggccta
    10681 tggccccagc ttccacgatc tcgggcgtcg aggggcaagc tacgtggaca agatcctcaa
    10741 gggcgccaag ccgggcgacc tgcccgtcga gcagcccacg aagttcgagc tgatcatcaa
    10801 cctgaagacc gcgaaggccc tcggcatgac gatcccgccg tcgctcctgc agcgggcgga
    10861 ccaggtgatc gagtgatgga ccggcgggtg ttcctcggaa ccttggcagg gggcctcctc
    10921 gccgcgccgc tcgccgccga ggggcagcag cccgcaagga tggtcaagat agggtatctg
    10981 gaattcggat cggcagcccc tggtacgccg cggtttgagg cattccggcg agggctccgc
    11041 gaactcggct ggatcgaggg caagagcatt gccatcgtgg gccgggccgc cgaggggaac
    11101 gagaatcggc ttcccgagtt cacggttgat ctcgtcaagc tgaaggtgga cctcatcttc
    11161 gcctcgagca cgcccgcagc cttggcggct aagcaagtcg ccacgacgac acccatcgtc
    11221 atcgggtttg tcgccgatcc ggtagggagt ggacttgtcg ctagcttggc acgcccgggc
    11281 gggaacatca cagggtggac acacctggcg ggtctggagt tgaacgcgaa acgtctcgaa
    11341 atactcgcac aggccgtccc cggggccgtt cgaattggtg ccctctggaa tcaagcaaac
    11401 ccgatccatg gaccgggatt gaaggaggtc gaagctgtag cccgaaggct cacggtggag
    11461 cttcacgcgg tgggggtacg cgaccccaaa gagctcgagg gggcattttc aacgctggcc
    11521 caacagcgcg tgcaagcgct cacagtgcct ccggacggga tgttccttgc tcaccagtca
    11581 cggatcatcg acctggcggc gaagcaccga atcccgacga tgtacggcgt cacggagctg
    11641 acgaaggctg gcggcttgat ggcctacggg acgaatctac tcgacatgta ccggcgcggg
    11701 gcgctcttcg tagacaaaat tctgaaggga gccaagccgg gtgacctgcc ggtcgagcag
    11761 ccgacgaagt tcgaccttgt gatcaacctc aagaccgcca aggccctcgg cctgacgatc
    11821 ccccagtcgg tcttgcagcg ggcggatcag gtgatcgagt gagtcgcaca ctgaaccgag
    11881 atgcccgcac cgagtctccc gaccgtcgac ctcagcagcc cacgggccac aagacgaacc
    11941 cctcgctacc gacattgcga aagctcgcca aggccctcgg cgtgccggtg acggagttgt
    12001 tgggatgacc tggccgagga cgatgaccga tttgcttcac ctcgtactca ccgtcgtggg
    12061 catcgcgcag ggcgttgtgt atgtcggcct ggcgctcctc ctggtcgcgt tcatctggtg
    12121 ggtcttcgca gtgcagcgaa acccggaccg gaccttctac gccgccacgc gtgccgtcct
    12181 gctcttggcc cttctggccg cgctggttct aggagccgtc ccatttatcc ggtggatgcg
    12241 gcagctcacg agttgagcat ggatgctgta gaccgcgagc agctccggcg cgcctgtgcc
    12301 gacgcgctcg ggcaagagaa cgcgatcacc tggcacctga tccacgtgct cgagagctcg
    12361 agaagggcgt gacgtagtgg ccgcgcacat ttccacgcgc ccggcggcgg gagaagctgg
    12421 cggagtactt gaagtgaggg gctacgttcg tgtccgcggc atctggcgga gccgcgccac
    12481 ccgccgacgg agtcgacgcg ccgctaactt ctccaccgcc cgtcgagccg cccagaccac
    12541 ccgaccgatc atgataaatc tgccgggtgc acgcagcgtg cccgcggcac attcctttgt
    12601 gcggctggac ttagcagccc gggccgtctt tccgctgagc cccggccttc ccaccatggg
    12661 cgccacgatg acatttctcg gaagggggaa gacgcacgtg gctcggccga ggcccgaccg
    12721 gccacagggt gaccctccgg tacgcccatc tgagccccga ccggctccga gaagccgtcg
    12781 ccagcctcga agcgttcagc acaaagtcag cacacagtgc taaaatcaag gccgaccgcc
    12841 tcgtaagtcc tgatgccccc gtagctcagg tggatagagc agcagtttcc taaactgcgt
    12901 gccgggtgtt cgagtcaccc cgggggcacc actttcagtg gaccgcttca acgcgaccct
    12961 tgaagcgcga ttcttcagac caaacgtaac ctcgcggagg gcgccctcga cgaaccctcc
    13021 cttgccttcg tagtcccagc cgcccctacc gtctggagcg actctgatcg aaccggggag
    13081 acaacgccgc agcatctgcc gcgcagctac agggtccgcg tcgagtaggt tgcgggtctc
    13141 ctcctcacgt acgttgggcg cattcggagc ggttagaagg tgcgctgcta cgtcgtgccg
    13201 ctcgacggca agggccggcc gccgccggcg ctctgcgagc cgtgagcccc cgtacggctc
    13261 gaacgctgcg ggtctggctg ggcagccccg cttccctgat gacgtctaat gtcggcagga
    13321 gcgcgcccgg aacggccgag tccgcccact tcgactttgc ttccagatag gccgcgccgt
    13381 gggcgctgtc atggcccctg gcggctcgtt cgttcgacca cccaat
//
LOCUS       DASUFA010000433         6392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_332212, whole genome shotgun sequence.
ACCESSION   DASUFA010000433 DASUFA010000000
VERSION     DASUFA010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332212"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFX87_15745"
     CDS             <1..271
                     /locus_tag="VFX87_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996435.1"
                     /translation="RFYPELATNQSRVADAELTKMLVAQRREMDPKKRKQIVDDIQRY
                     LADKAYYVYVPQWPQYVAHPPYVKGFRHHDGYGLGMRLIYTWLDK"
     gene            306..1250
                     /locus_tag="VFX87_15750"
     CDS             306..1250
                     /locus_tag="VFX87_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996436.1"
                     /translation="MKRYLLRRLALAIPTLFLVSVLVFALMRLMPGDVATRMVEGHAY
                     APTLAALRHDLGLDRPVHAQYLEWIGGIVTRGDFGRSYWTRQPILDEFVRRFPVTLEL
                     ALLTILVSVVLGVLVGIVSAVRQDSVSDYVGRILAILALSIPYFGLAVLVVVLPSIWF
                     KWTPVWSYVPFTTDPLENLKIMLVPALVFGVTRAGPVMRIMRSALLDVLRQDYIRTAW
                     SKGLAERPIILRHALKNAMIPVISLIGLQMPLYIGGSVIIESIFRLPGVGLFFFEALT
                     RLDYPVVQSVNLIIAALVVGLNLLIDLSYAFLDPRIRY"
     gene            1306..2169
                     /locus_tag="VFX87_15755"
     CDS             1306..2169
                     /locus_tag="VFX87_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996437.1"
                     /translation="MPGAPRPWWIDVWVRMLAGKPLGTIGGVIVVAMLAAAVLADWIA
                     PYGYAQTSLRQRFIAMSAAHWLGTDELGRDLLTRLIYGARISLYVGFGAIGIGSVLAT
                     LIGMVSGYLGGRFDLLVQRGVDAWMAFPPLLLLLSIMSLLGPSVWNITIVLGVAFGIQ
                     NSRVVRGVALLVKEHTFVESARALGAGHVRTTLTHILPNVMPTIIVVATTGLSTVVLT
                     EASLSFLGLGVPPPYPTWGGMLSLAGLDHMYRAPWLAIWPAVALSLAVFGFNMLGDAL
                     RDLLDPRLKGG"
     gene            2302..2991
                     /locus_tag="VFX87_15760"
     CDS             2302..2991
                     /locus_tag="VFX87_15760"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996438.1"
                     /translation="MALRAFLASVALGSLLVTVIVTGADAGSPTTTLQATFAQANKII
                     ADPTLEDRPLERIVAIRTLFSKVFDFRGASEDVLGPEWKARTPVEQREFTSIFSGFVQ
                     RSFVHWLAAVAEIDANGISVSYLGESVQRDRAGVRTAIAGRGGRQIHLDHWMVYQGNR
                     WLVRDITIDGVSLITNYRAQFDRVIRASSYRNLVVRMQERVGTELPRPASGGELLGAE
                     VTVPLFLYEMR"
     gene            complement(2999..4198)
                     /locus_tag="VFX87_15765"
     CDS             complement(2999..4198)
                     /locus_tag="VFX87_15765"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996439.1"
                     /translation="MPPAARAFPWYLAGISSWFASSGMQVIVFPWLVTVVLHEPAARV
                     GVAQMSLMAPSIFLMLLGGAVADRADCRRLLVRGHLVAALPPLLLGAAIAWGWLAYGG
                     LIAYALAVGAAGAFVMPARDAMLTRVVAGGLGRAVAVMTATQFAAQLVGIAVGGSAGA
                     IGAPTLLLLQAAVLAFGGIAALRLPAAPPMAGAAALSRLGAMRDGLRAAAASPVLVPV
                     LIANLAVGVLYVGAFLVILPLIVRDVYGGGSAQLSLVSLGFWGGTIGSTLVQIRLGAL
                     RRPGRAIVVALLLGAGVLAAMVWPSPFPAFVALCLVWGLGAGVVMTQARTLVQSAAPE
                     THRARILSLFQLGLMGGAPVGALLIGYLAALTGPRGAAIYPATCMLLVLAGLLLRSRL
                     WHQGAAR"
     gene            complement(4204..4563)
                     /locus_tag="VFX87_15770"
                     /pseudo
     CDS             complement(4204..4563)
                     /locus_tag="VFX87_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            complement(4636..5793)
                     /locus_tag="VFX87_15775"
     CDS             complement(4636..5793)
                     /locus_tag="VFX87_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996440.1"
                     /translation="MDFKLTPTQEAFREEIRGFLRSELVAEPRRVREDGWVVGFSRAF
                     SEKLGKRGWLGLTWPRQYGGQARSVLERLILTEELLRAGAPVAAHWLGDRQVGPSILR
                     FGTEAQRREILPRITSGAIVFCVGLSEPDAGSDAAAVTTQAVEDGDGFVIRGQKIWTS
                     FAHQADYCYLVARTDASGPKHKGISEFIVPMATPGITVRPLLDMTGEHHFNEVFFEDV
                     RVPSTALIGQKDRGWYQIAVQLDYERGGIERVLSNWPLLEDALALLQGSAVLRDPVVR
                     DRLAGLRVDLETARLMVYRIAWLADHGRVPNVEAAVAKSFGTELEQRIAETVAGLFQM
                     SALGLAGTARAALGGRVARAVLYAPAYTIQGGTSNILRNIIAQRGLGLPAD"
     gene            5938..>6392
                     /locus_tag="VFX87_15780"
     CDS             5938..>6392
                     /locus_tag="VFX87_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4996441.1"
                     /translation="MGARQREISVSEFFTKNRHLLGFDNPRKALLTCVKEAVDNALDA
                     AEEAGILPEVAVTLEVASSGDEAPVAASQATRFRVTVIDNGPGIVRQQIPPIFAKLLY
                     GSKFHRLRMSRGQQGIGISAAGMYAQLTTGKPVQITSSTGPRAATHYFEV"
BASE COUNT          958 a         2381 c         2117 g          936 t
ORIGIN      
        1 ccgcttctat cccgagctgg cgaccaacca gagccgggtg gccgacgcgg agctgaccaa
       61 gatgctggtc gcccagcggc gggagatgga ccccaagaag cgcaagcaga tcgtggacga
      121 catccagcgc tacctggccg acaaggcgta ctacgtctac gtgccgcaat ggccgcagta
      181 cgtggcgcac ccgccctacg tgaagggctt ccggcaccac gacggctacg ggctcggcat
      241 gcgcctgatc tacacgtggc tcgacaagta ggcggcggcg cctagcccga gccgcggccc
      301 acgccgtgaa gcggtacctg ctccgccgcc tggccctggc gatcccgacg ctcttcctgg
      361 tgagcgtcct ggtcttcgcc ctcatgcgtc tcatgccggg cgacgtggcc acgcgcatgg
      421 tggaaggcca cgcctacgcc ccgaccctgg ccgccctccg ccacgacctc ggcctcgacc
      481 ggccggtaca cgcgcagtac ctcgagtgga tcggcggcat cgtcacccgc ggcgatttcg
      541 gccgctccta ctggacgcgc cagcccatcc tcgacgagtt cgtgcggcgc ttcccggtga
      601 cgctcgagct ggcgctcctg accatcctgg tctcggtcgt gctcggggtc ctggtgggca
      661 tcgtctcggc ggtgcgccag gactcggtct ccgactacgt gggccggatc ctggcgatcc
      721 tggccctgtc catcccgtac ttcggcctgg ccgtgctggt ggtggtcctg ccctccatct
      781 ggttcaagtg gacgccggtc tggagctacg tccccttcac caccgatcct ctcgagaacc
      841 tcaagatcat gctcgtgccc gccctcgtgt tcggggtgac gcgggccggc ccggtgatgc
      901 gcatcatgcg ctcggcgctg ctcgacgtgc tgcgccagga ctacatccgc accgcgtggt
      961 ccaaggggct cgcggagcgg cccatcatcc tgcgccacgc gctcaagaac gcgatgatcc
     1021 cggtgatcag cctgatcgga ctgcagatgc cgctctacat cggcggctcg gtgatcatcg
     1081 agtccatctt ccgcctgccc ggggtcgggc tcttcttctt cgaggccctg acccgcctgg
     1141 actacccggt ggtccagtcc gtgaacctga tcatcgcggc gctggtggtc ggcctcaacc
     1201 tcttgatcga cctctcctat gccttcctcg atccccgcat ccggtattaa gtcggaggaa
     1261 gcctcgccct cgatcgcacc ggccaagccg gcgctcgaag cgcgaatgcc gggggcgccg
     1321 cggccgtggt ggatcgatgt gtgggtgcgg atgctcgcgg gcaagccgct cggcacgatc
     1381 ggcggcgtca tcgtggtggc gatgctggcg gcggcggtgc tggccgactg gatcgcgccc
     1441 tacggctacg cccagacgag cctccgccag cgcttcatcg ccatgagcgc ggcgcactgg
     1501 ctcggcaccg acgagctcgg ccgcgatctc ctgacccggc tcatctacgg cgcgcgcatc
     1561 tcgctgtacg tgggcttcgg ggccatcggc atcggcagcg tgctcgccac cctgatcggc
     1621 atggtctcgg gctacctggg cggccgcttc gatctgctcg tgcagcgggg agtagacgcc
     1681 tggatggcgt tcccgccgct cctgctgctc ctgtccatca tgtcgctgct cgggcccagc
     1741 gtctggaaca tcaccatcgt gctcggcgtg gccttcggca tccagaactc gcgcgtggtc
     1801 cgcggcgtgg ccctgctggt caaggagcac acgttcgtgg agagcgcccg cgcgctgggc
     1861 gccggccacg tgcggacgac gctgacccac atcctgccga atgtcatgcc gaccatcatc
     1921 gtggtcgcga ccaccggtct ctcgacggta gttctgaccg aggcgagcct ctcgttcctc
     1981 ggcctgggcg tgccgccgcc ctatccgacc tggggtggaa tgctctcgct ggccggcctc
     2041 gaccacatgt accgcgcgcc ctggctcgcg atctggccgg cggtggcgct gtcgctcgcc
     2101 gtcttcggct tcaacatgct gggcgacgcg ctgcgcgacc tgctcgatcc tcgactgaag
     2161 ggcggctgac actctgacgg taatcgtcca tcccggacgg tcaccggcga ctcggaatcg
     2221 acagtaccgc cttcaatcct ttgcttttgg cctgcctgac ggcgctcctc cggtggcacg
     2281 tgcgatgcac tgatcggagc catggctctc cgtgccttcc ttgcctccgt cgcgctcggc
     2341 tctctcctcg tcaccgtcat cgtcaccggc gccgacgccg ggtcacccac caccacgctg
     2401 caggccacgt ttgcccaggc caacaagatc atcgccgacc cgacgctgga ggatcggccg
     2461 ctcgagcgca tcgtggcgat ccgcaccctg ttcagcaagg tcttcgactt ccgcggcgcc
     2521 tcggaggacg tgctcggacc ggagtggaaa gcgcgcacgc cggtcgagca gcgtgagttc
     2581 acgtcgatct tctcgggctt cgtccagcgc agcttcgtcc actggctcgc ggcggtcgcc
     2641 gagatcgacg ccaacgggat ctccgtctcc tacctgggtg agtcggtgca gcgggaccgc
     2701 gcgggagtac ggaccgccat cgcgggtcgg ggcggccggc agatccacct cgaccactgg
     2761 atggtctacc agggcaatcg ctggctggtg cgggacatca cgatcgacgg ggtcagcctg
     2821 atcacgaatt accgggcgca gttcgatcgg gtgatccggg cctcgtccta ccgcaacctc
     2881 gtggtccgga tgcaggagcg ggtgggcacc gagctgccgc gccccgcctc gggcggtgag
     2941 ctgctcggcg ccgaggtgac ggtgccgctg ttcctctacg agatgcgctg aacccgcctc
     3001 atcgcgccgc gccctggtgc cagaggcgcg agcgcagcag cagcccggcc agcaccagca
     3061 acatgcacgt ggcgggatag atcgccgcgc cccgcggccc ggtgagcgcg gccagatagc
     3121 cgatcagcag ggcgcccacc ggcgcgccac ccatcagacc gagctggaag agcgagagga
     3181 tccgcgcgcg gtgcgtctcc ggcgccgccg actgcaccag cgtccgcgcc tgggtcatga
     3241 ccacgcccgc ccccaggccc cagacgagac agagcgccac gaacgccggg aagggactcg
     3301 gccataccat ggccgcgagc acgccggccc cgaggaggag ggcaaccacg atcgcccgcc
     3361 ccgggcgccg gagggccccc aggcggatct gaacaagggt ggacccgatc gtgccgcccc
     3421 agaagcccag actcacgagg gagagctgcg ccgagccgcc gccgtacacg tcgcgcacga
     3481 tcagcggcag gatcacgagg aacgcaccca cgtagagcac gcccaccgcc aggttcgcga
     3541 tcagcacggg gaccaggacc ggcgacgcgg cggccgcccg cagcccgtcg cgcatggcgc
     3601 cgagccggct cagggccgcg gccccggcca tcgggggcgc cgcgggaagc cgcagcgccg
     3661 cgatgccccc gaacgccagc accgccgcct gcagcagcag gagcgtgggc gcgccgatcg
     3721 cgccggcgct gccgcccacc gcgatcccca cgagctgcgc cgcgaactgc gtcgcggtca
     3781 tcaccgccac cgcgcgccca agcccgcccg cgaccacgcg cgtgagcatc gcgtcgcgag
     3841 ccggcatgac gaaggcgccc gcggcgccga ccgcgagcgc gtacgcaatc aggccgccgt
     3901 aggccagcca gccccacgcg atggccgcgc ccagcaggag cggcggcagg gccgccacga
     3961 gatgcccgcg caccagcagc cgccggcaat cggcgcgatc agcgaccgcc ccgccgagca
     4021 gcatcaggaa gatcgacggc gccatgagcg acatctgggc gacgccgacc cgcgccgccg
     4081 gctcgtggag caccaccgtg accagccacg ggaacacgat gacctgcatc ccggagctgg
     4141 cgaaccatga gctgatcccg gccagatacc agggaaaggc gcgcgccgcg ggcggcaccc
     4201 ggctcagacc agccgcgtgc ggatgtcgat ctcggagacc agggtgcggt gcaccgggca
     4261 gcggttggcg atctcgagca gccgctcgcg ctggcccgcg tcgagcggcc cgcccaggct
     4321 gatctcccgc tcgatccggt cgagcttgcc ctccttcgtc tcgcactcgg cacagtcctg
     4381 ggcgtggatc ttcgcgtgct gcagccgcac cgtgacctcc tccagcggcc actgcttacg
     4441 ccgcgcgtac atcgagaccg tcatggacgt gcaggcgccg agcgcggcca gcagcagctc
     4501 gtagggcgag gcgcccgtgt ccgtcccgcc cgcctccacc ggctcatcgg caaccagccg
     4561 gtgccgttcc gccgcgatct cctgcgcgaa cccggtcgcg ccgcctctca cgatcacgat
     4621 ggccatgcga gcctctcagt cggcgggcaa gccgagcccg cgctgcgcga tgatgttcct
     4681 caggatattg ctcgtcccgc cctggatcgt ataggcgggc gcgtagagga cggcgcgcgc
     4741 gacgcgcccc ccgagcgcgg cgcgcgcggt cccggccagg cccagcgcgg acatctggaa
     4801 caggcccgcg acggtctccg cgatgcgctg ctcgagctcg gtcccgaacg acttggccac
     4861 cgccgcctcg acgttgggca cccgcccgtg atcggcgagc cacgcgatgc ggtagaccat
     4921 caagcgggcc gtctcgagat ccacgcgcag tccggccagc cggtcccgta ccaccgggtc
     4981 ccgcaggacc gcgctgcctt gcaggagggc gagcgcgtcc tccagcagcg gccagttgct
     5041 cagcacgcgc tcgatacccc cgcgctcgta gtccagctgc accgcgatct ggtaccagcc
     5101 acgatccttc tgcccgatca gcgcggtcga ggggacccgt acgtcctcga agaagacctc
     5161 gttgaagtgg tgctcgccgg tcatgtcgag gagcgggcgc accgtgatcc ctggagtcgc
     5221 catcggcacg atgaactcgc tgatcccctt gtgcttgggc ccgctcgcgt ccgtgcgcgc
     5281 caccaggtaa cagtagtcgg cctggtgggc gaagctcgtc cagatcttct gaccccggat
     5341 cacgaaaccg tcgccgtcct cgaccgcctg cgtggtgacc gccgccgcgt cggagccggc
     5401 gtccggctcg ctcaggccga cgcagaagac gatggcgccc gacgtgatcc ggggcaggat
     5461 ctcacggcgc tgcgcctcgg tcccgaagcg caggatggac gggccgacct gacgatcccc
     5521 gagccagtgc gccgccaccg gggcgcccgc gcgcagcagc tcctcggtga ggatcaggcg
     5581 ctcgagcacc gagcgcgcct ggccgccgta ctgccgcggc caggtcagcc cgagccatcc
     5641 gcgcttgccg agcttctccg agaacgcccg ggagaatccg acgacccagc cgtcctcgcg
     5701 gacgcggcgg ggctcggcga ccagctccga gcgcagaaat ccccggatct cttcgcgaaa
     5761 ggcctcctgc gtcggggtca gcttgaagtc cacgcccacc gaacagtaca ggctcgcgag
     5821 gcgatggtca aaaaaaggcg gctggagcgc acggatttga tatcaaggag tggcatgtcc
     5881 gccgcgctcg ccaggccgcc gtccgccgag cccaaggccg ccccatccgc cgccgagatg
     5941 ggcgcccgcc agcgggagat ctccgtctcg gagttcttca ccaagaatcg tcatctgctc
     6001 ggcttcgaca atccgcgaaa ggcgctcctc acgtgcgtca aggaagcggt ggacaacgcg
     6061 ctcgatgccg ccgaggaggc cggcatcctg cccgaggtgg ccgtcacgct ggaagtcgcg
     6121 tcgagcggcg acgaggcgcc ggtggcggcc agccaggcga cccgtttccg cgtcacggtc
     6181 atcgacaacg ggcccggcat cgtccgccag cagatcccgc cgatcttcgc caagctcctc
     6241 tacggctcga agttccaccg gctgcgcatg agccgcggcc agcagggcat cggcatctcc
     6301 gcggccggca tgtacgcgca gctcacgacc ggcaagccgg tgcagatcac ctcgagcacc
     6361 gggccgcggg ccgcgacgca ctacttcgag gt
//
LOCUS       DASUFA010000434         4902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_332755, whole genome shotgun sequence.
ACCESSION   DASUFA010000434 DASUFA010000000
VERSION     DASUFA010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332755"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFX87_15785"
     CDS             <1..555
                     /locus_tag="VFX87_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4996442.1"
                     /translation="SPDLEDVRGQPRARRALEIAAAGGHSLLMIGPPGTGKTMLARRV
                     ASILPPLSLERPSRAPPSGAWPGSWGPSRVCSPGGRSAARITPHEAGLIGGGGVPHPG
                     EINFAHHGVLFLDELLLCVEDALEPAGRQSVRDSIRNPRSVGEHPHGLGEARLSVFCP
                     RSLVHGVAHDTHWSPWDHVESRTC"
     gene            742..2358
                     /locus_tag="VFX87_15790"
     CDS             742..2358
                     /locus_tag="VFX87_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX4996443.1"
                     /translation="MTTRWPEARAPRGMVATPHLLATQSGLAALRAGGNALDAAIAAA
                     ATIAVVYPHMNGLGGDNVWLIYDARQQRLRALCGVGRAARAASLDWYAGRRITSAIPA
                     RGGPAALTVPAAVDGWWQAHEYSRGAMGSAIGWKALLADALLYAREGFQASDGQRIPP
                     PREPDLFTERAPEEIRRALWPLYHPDALRRGPLVQRDLARTLEAVRDGGPEAFYRGDV
                     ARRLVTAAAAAGSPLTVEDLAEHRSEWMEPLRIPYAEGQVASFPPPTQGISALAVLGM
                     TERFDMSALPEADYIHVLVEATKLAFEDRDRYLTDPAHMRVSPVELLAPERLQARARL
                     ISRDGARRPQPTEPAGGDTIALVTADREGNAVSLIQSLYFTFGAALLAGDTGVVLQNR
                     GSFFSLDPGHVNALAPGKRTMHTLIPSMYLEGGRPRFVYGTMGGEGQPQTQAAILTRR
                     LFRRLGPQAAIEAPRWLYGRTWGTASRALSLEGRYPPELAAELAGRGHEVAVGGEWDD
                     LFGHAHGIWMEDGGLIGGSDPRADGGALGY"
     gene            complement(2434..4002)
                     /locus_tag="VFX87_15795"
     CDS             complement(2434..4002)
                     /locus_tag="VFX87_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4996444.1"
                     /translation="MAVASFPTAASAQKPISVQEALQRAKPAVVLVVAEVASEVTLDC
                     GSGPQKITPPVFRETGTGWFLDADGWLITNGHVVQPAYEPPRWLANQQAQRAVTTACL
                     PQVLKRMGLQPGDAPEREEAAKRKLLDTVLPTTKVNLQPQIFVVTAKGRLKAEVKKYS
                     PPVSEMSARDLALLKVAGDSFPVLPLADSRGVQVGDPVHILGFPGVVLSHELLSKSTM
                     VDSVVTNGAVSAIQADASNNPLIQTDASAAWGNSGGPTVDDRGKVVGVLTFVSLAPGA
                     EGAIVQGFNFVIPSAAVADFVRGTPVKINGASKFNDVWYAGLGAFYAEDYPGALRRFE
                     EADKLHPNLPDVRRKLVEAREYVKNPPPRPFPWFWVAVGVTLVSAGGFGGQWLHRWNK
                     NRYRVQPFEVVRMIEAGKSPNILDARKSDSYEQLPLKIPGSVRVVPEDLANSMAGLDL
                     DPTRPVVAYCSCPDEETSARVARDLRKLGFKDVRILKGGLGAWTNAGLPIETRSDLSQ
                     VGLELYKALAGGNA"
     gene            4080..4472
                     /locus_tag="VFX87_15800"
     CDS             4080..4472
                     /locus_tag="VFX87_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4996445.1"
                     /translation="MSRPIGEVPTRTFQPGDVIFREGDDAKGEAFMVHVGRVEIRKQL
                     GGEERLLRTLAKGELLGHIALFRNAPRSATAIAADAVTLMVIPANRLDHLVRANPALA
                     VALIRDLAASMLAAEDRLRECEEKLGKK"
     gene            complement(4477..>4902)
                     /locus_tag="VFX87_15805"
     CDS             complement(4477..>4902)
                     /locus_tag="VFX87_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953608.1"
                     /note="catalyzed the formation of 2-ketoglutarate from
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX4996446.1"
                     /translation="DVEAGPNAVLAFAREGYTLGTVRPGEFMGTLGYAGFWHMARKYW
                     RVGAFEMYRSASKAAFVRSLQKLVPDIAASDLERGGAGVRAQAVAPDGSLVDDFKISV
                     TAGAVHVVNAPSPGATASLAIGRHVAGVAAETFGLGAAA"
BASE COUNT          690 a         1799 c         1733 g          680 t
ORIGIN      
        1 agtcctgatc tcgaggacgt ccgcggtcag ccgcgagctc gccgggcgct cgagattgcg
       61 gcggccggcg gccacagcct gctgatgatc gggccgcccg gcaccggcaa gaccatgctg
      121 gcccggcggg tggcctcgat cctgccgccg ctatcgctcg agaggccatc gagggctcca
      181 ccgtctggag cgtggcccgg ctcctggggc ccgagcaggg tctgctcacc aggcggccgt
      241 tccgcagccc gcatcacacc gcacgaggcg ggcctcatcg gcggcggtgg ggtcccgcat
      301 ccgggcgaga tcaacttcgc ccatcacggg gtgctctttc tagacgagct gctcctgtgc
      361 gtcgaggatg ccctcgagcc ggcgggccga cagtccgtac gtgactcaat tcggaaccca
      421 cggtctgtgg gtgagcaccc tcacgggctg ggggaggccc gtctgtccgt gttctgtccg
      481 cgttccctag tccatggggt cgcacatgac acgcactggt cgccctggga tcacgtagaa
      541 tcgcgcacat gctgacacca gagacatgcg ggaaggcgag agctacgtgc atcacctgcg
      601 gcaccacgac cggctgctcg taggcctggc ggccgccgcc ccctcaccct accctctccc
      661 cctcacgggg aagagggttc ttcattcctc ccgctctccc ctctggggag agggccgggg
      721 tgaggggctg gcgccgctcc catgaccacc cgctggcccg aggcgcgcgc cccgcgcggc
      781 atggtggcca cgccgcacct cctcgccacc cagtccggcc tcgcggccct gcgggcgggc
      841 ggcaacgcgc tcgacgcggc gatcgcggcg gcggccacca tcgcggtggt ctacccgcac
      901 atgaacggcc tgggcggcga caacgtgtgg ctcatctacg acgcgcgcca gcagcggctc
      961 cgcgccctct gcggggtggg ccgcgccgcc cgcgcggcca gcctcgactg gtacgcgggg
     1021 cgcaggatca cctccgccat ccccgcgcgg ggcgggcccg ccgcactcac cgtgccggcg
     1081 gccgtggacg gctggtggca ggcgcacgag tacagccgcg gcgccatggg ctcggcgatc
     1141 ggctggaaag cgctgctggc cgacgcgctc ctgtacgcgc gcgaggggtt ccaggcctcc
     1201 gacggccagc gcatcccgcc gccgcgcgag cccgatctct tcaccgagcg cgcgcccgag
     1261 gagatccgcc gcgcgctctg gccgctctac cacccggacg cactgcgccg cgggccgctg
     1321 gtgcagcgcg acctggcccg cacgctcgag gcggtgcgcg acggaggccc cgaggccttc
     1381 taccgcgggg acgtggcccg gcgcctcgtg acggcggcgg ccgcggcggg cagcccgctc
     1441 accgtcgagg acctggccga gcaccggtcg gagtggatgg agccgctgcg catcccgtat
     1501 gcggaggggc aggtcgcgag cttcccgccg ccgacccagg gcatctcggc gctcgcggtg
     1561 ctgggcatga ccgagcgctt cgacatgagc gcgctgcccg aggccgacta catccacgtc
     1621 ctggtggagg ccaccaagct cgccttcgag gaccgcgacc ggtacctcac cgacccggcc
     1681 cacatgcgcg tgagccccgt cgagctgctg gcccccgagc gtctccaggc gcgcgcccgg
     1741 ctgatctcgc gcgatggcgc acgccggccc cagcccaccg agcccgcggg cggcgacacc
     1801 atcgcgctcg tcaccgccga ccgcgagggc aacgcggtct ccctgatcca gtcgctctac
     1861 ttcaccttcg gcgcggcgct gctggcgggc gacaccgggg tggtgctgca gaaccgcggc
     1921 tcgttcttct cgctcgatcc gggccacgtc aacgcgctcg ccccgggcaa gcgcaccatg
     1981 cacaccttga tcccgtccat gtacctcgag ggcgggcgcc cacgcttcgt ctacggaacc
     2041 atgggcggcg agggccagcc gcagacgcag gccgccatcc tcacccgccg cctgttccgg
     2101 cggctgggtc cgcaggccgc catcgaggcc ccgcgctggc tctacgggcg cacgtgggga
     2161 accgcctcac gcgcgctcag cctggaggga cgctacccgc cggagctcgc ggcagagctg
     2221 gccggccgcg ggcacgaggt cgcggtcggc ggggagtggg acgatctctt cggccacgca
     2281 cacggcatct ggatggagga cggcggcctg atcggcgggt cggacccgcg ggcggacgga
     2341 ggggcgctgg gctattgagg gccttccctc ctcatccccg tctcctctca ggggagaggg
     2401 cagggtgagg gggcccgggg tgaggggtcg gtgtcaggcg ttgccgccgg ccagcgcctt
     2461 gtacagctcg agcccgacct gggagaggtc cgagcgcgtt tcgatcggca gccccgcgtt
     2521 ggtccaggcg ccgaggcccc ccttcaggat ccgcacgtcc ttgaagccca gcttgcgcag
     2581 gtcacgggcc acccgggcac tggtctcctc gtccgggcag gagcagtagg cgaccacggg
     2641 gcgcgtggga tcgaggtcga ggcccgccat gctattggcc aggtcctccg gcaccacccg
     2701 caccgagccg ggaatcttga gcgggagctg ctcataggag tcggacttgc gcgcgtcgag
     2761 gatattcggc gacttgccgg cctcgatcat ccgcaccacc tcgaagggct ggacccggta
     2821 gcggttcttg ttccagcgat gcagccactg accgccgaag ccgcccgcgc tcaccagcgt
     2881 cacgccgacc gccacccaga accacgggaa gggcctgggc ggcgggttct tcacgtactc
     2941 ccgggcctcg accagcttgc gccggacgtc gggcaggttc gggtggagct tgtcggcctc
     3001 ctcgaagcga cgcagcgcgc cggggtagtc ctcggcgtag aaggcgccca ggcccgcata
     3061 ccagacgtcg ttgaacttgc tggccccgtt gatcttgacc ggggtgccgc gcacgaagtc
     3121 ggccaccgca gccgacggga tcacgaagtt gaagccctgg acgatggcac cctccgcgcc
     3181 cggcgccagc gagacgaagg tgagcacccc caccaccttg ccccggtcgt ccaccgtggg
     3241 gccgcccgag ttcccccatg cggccgaggc atcggtctgg atgaggggat tgttgctcgc
     3301 gtccgcctga atcgcggaca cggcgccgtt ggtcacgacg gagtccacca tggtggactt
     3361 gctgagcagc tcgtgggaga gcaccacccc cgggaagccg aggatgtgca cggggtcgcc
     3421 gacctggaca ccgcgggaat cggccagggg cagcaccggg aaggagtccc ccgcaacctt
     3481 caggagggcc aggtcccggg ccgacatctc gctcaccggc gggctgtact tcttcacctc
     3541 ggccttcagc cgccccttgg cggtgaccac gaagatctgg ggctgcaggt tgaccttggt
     3601 ggtcggcagc acggtgtcga gcagcttgcg cttggccgcc tcctcgcgct ccggcgcatc
     3661 ccccggctgc agccccattc gcttgagcac ctgggggagg caggccgtcg tcaccgcccg
     3721 ctgggcctgc tggttcgcca gccagcgggg cggctcgtag gccggctgca ccacgtggcc
     3781 gttggtgatc agccagccgt cggcgtccag gaaccagccg gtgccggtct cccggaaaac
     3841 gggcggtgtg atcttctggg gcccggatcc gcagtcgagg gtgacctcgg aggcgacctc
     3901 ggccaccacc agcaccaccg ccggcttggc gcgctggagg gcctcctgga ccgagatggg
     3961 cttctgggct gaggcggcgg tggggaacga ggcgacggcc atgacgacgg ccgtcccgaa
     4021 cagcgctgaa cgcagtatca taggtccact atgggtcatt ctacaccgag acaggagcca
     4081 tgagccggcc gatcggggaa gtgcccacca ggacgttcca gcccggcgac gtgatctttc
     4141 gcgagggcga cgacgccaag ggcgaggcgt tcatggtgca cgtgggccgc gtggagatcc
     4201 gcaagcagct cggcggcgag gagcgcctgc tgcgcacgct ggccaagggc gagctgctcg
     4261 ggcacatcgc gcttttccgc aacgcgcccc gctccgccac cgcgatcgcc gccgacgcgg
     4321 tcaccctgat ggtcatcccc gccaaccggc tcgatcatct ggtgcgggcg aacccggcgc
     4381 tcgccgtcgc gctgatccgt gatctggccg ccagcatgct cgccgcggaa gaccggctgc
     4441 gcgagtgcga ggagaagctg gggaagaagt agccgctcag gccgcagcgc cgagaccgaa
     4501 ggtctcggcc gcgacgcctg cgacgtggcg cccgatggcc agcgacgcgg tggcgcccgg
     4561 ggagggcgcg ttcaccacgt gcacggcgcc cgccgtcacg ctgatcttga agtcgtccac
     4621 cagcgagccg tccggggcca ccgcctgggc gcggaccccc gccccgcccc gctcgagatc
     4681 ggacgccgcg atgtcgggca cgagcttctg cagcgagcgc acgaaggccg ccttgctggc
     4741 cgagcggtac atctcgaagg caccgacccg ccagtacttg cgggccatgt gccagaagcc
     4801 cgcgtagccg agcgtgccca tgaattcgcc gggccgcacc gtgccgaggg tgtaaccctc
     4861 gcgcgcgaag gcgagcaccg cgttcggccc cgcctccacg tc
//
LOCUS       DASUFA010000435        13109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_545384, whole genome shotgun sequence.
ACCESSION   DASUFA010000435 DASUFA010000000
VERSION     DASUFA010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545384"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFX87_15810"
     CDS             <1..693
                     /locus_tag="VFX87_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX4996447.1"
                     /translation="RLETCETCRGAGVEAGSQPEVCGTCRGQGQVRFTQGFLTVARPC
                     PNCRGEGRIIKHPCPGCRGQGRVSHERLLKVTIPAGVENGNQLRLNGEGESGLQGGPA
                     GDLYVVLHLKPHEIFVRDGAHLICELPLTFPQVALGDEVEVPVLDGAAKLKIPAGTQP
                     GQRLVLKGKGMPHLRGRGRGDAVYEVVVEVPTQLTVRQRELLEEFRRSHDGDSSPRAS
                     KFVERMKQLFGS"
     gene            693..1577
                     /locus_tag="VFX87_15815"
     CDS             693..1577
                     /locus_tag="VFX87_15815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX4996448.1"
                     /translation="MAFWQLTLPASADSEDALTNFLWEQGALGVVEEEAPGVPPRLRA
                     FFAESMSSTRLLAAVQDYQASLRSLGFAIESQGTEITPLLDEAWASAWQQSFPAREVG
                     RRLLVLPPWIAPPVRTDGVPRLTVIIEPGRAFGTGHHGTTEGCLVLLEEALAATPGAA
                     TLDIGTGTGILAIAALKLGAPTVLAIDVDPDAVSATQVNAGRNGCTGLTVRLAEPQEI
                     PNRFPLVLANLLTHTHLALAPQYARLVAPGGSLVLGGMLQDEDGRVSEALAGAGFTPR
                     SRLVLEGWASLRLEAPPA"
     gene            1574..2317
                     /locus_tag="VFX87_15820"
     CDS             1574..2317
                     /locus_tag="VFX87_15820"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX4996449.1"
                     /translation="MTRFLHLGQDAVDGDRVAFEAAAAHHLGRVLRAAVGDVVQAVDH
                     GGALLSVRLTAIAARRAEGLIVSRAPLATESPLDLTLAQGVPKGDKMDGIVRMATELG
                     VSRVIPLLTARTVVRLEPARWTSRLGRWQRIAKEAAQQSGRAAVPEIAAPRDVSSWAR
                     EAGAAGLLVCLWEEEREGLDKRLPAGPCPRVTVVVGPEGGLTADEVRGLTDAGAVVAG
                     LGPRLLRTETAGAVAVALLQSRYGDLGTA"
     gene            2319..2723
                     /locus_tag="VFX87_15825"
     CDS             2319..2723
                     /locus_tag="VFX87_15825"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX4996450.1"
                     /translation="MQLAVEAATLPDAFARIALSLFAVAVDPASVDPAEVREVRAHGA
                     GVLALLRRWLEECLYVHEVEGFACRSIEFVVFEASPTAGGEPFRLHAFLRGEPVEPAR
                     HQVHGLIRGIGSDGLTVEPFVEGFRVRARLEL"
     gene            2881..3795
                     /gene="lpxC"
                     /locus_tag="VFX87_15830"
     CDS             2881..3795
                     /gene="lpxC"
                     /locus_tag="VFX87_15830"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX4996451.1"
                     /translation="MDSQQTVRRPVSLDGIGLHSGETAKMTIAPAAADTGILFRAADG
                     TLIPANADHVVDTNSATTVGAFGVRVRTIEHLMAAAAALGIDNLMVDIDGPEVPAADG
                     SAKPFVDLLRSAGRVSLAAPRRAITVSEPIRVGTESRWIEVLPADSLRISYTLDNNHP
                     IIGLQAGTFGITEEVFCDELAAARTYGFLRDVPAMRQNGLARGGSLDNAIVVGKRSVL
                     NDSLRFPDEFVRHKILDLVGDLFLLGRPLRAHVVGRNAGHALNYQVVAAIQKALAADR
                     RRVAARAARPVPAPVAASDGFLPGVAAL"
     gene            3897..4481
                     /locus_tag="VFX87_15835"
     CDS             3897..4481
                     /locus_tag="VFX87_15835"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX4996452.1"
                     /translation="MRTYRRRLIRTFALASGLIAVLLGAPAAEAEVRISNLAVFLNDY
                     DVTVTVVLFGAVPDALHESIHTGIAAHVRYYVELWQHTRMGVDRRLQARMVERQLTYN
                     VLTKEYKVAPLKGEQREPLLTKDLREAQRVISELRVGQLAPVAALDKQELYYVRVRSD
                     VSLGGVNSWFARMTGEAEETPWVQSSLLTPMRSQ"
     gene            4481..6724
                     /locus_tag="VFX87_15840"
     CDS             4481..6724
                     /locus_tag="VFX87_15840"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4996453.1"
                     /translation="MPLTPRPSPVDEQEKRKRNLLIITGILVLLVVATVFEVGIRAPQ
                     IPFASNLIVFFLFNLNLIVFLLLLVLLFRNLVKLSFERRQKIIGSKFKAKLVLTFLAL
                     ALVPGILIFLIASNFITTSIEGWFKPQVEKPLDQAMEVAQTYYQSLETTALRHGRYMG
                     RVIERDGLLAESKREALASYLAEQQDRLGVAAITVFNRDGQELVHVKNPVLAKVPTRE
                     ANSEHVRQALSGQEITTVHELDNGDMIQAMVPVRDGDQAVPATIVVAIHVPQRLEARL
                     RNISQAFQEYKQLRLLKNPIKGIYILLFLLMTLIIVFSATWFGLYLARGITEPIQMLA
                     EGTREVAAGNLNYKVTVRADDEIGILVDSFNRMTGDLAASQSKLEETYKDLQAKHGEV
                     EDRRRYTETVLEAVATGVVSLDAEGQVTTINGAAERLLGLTAAQIQGRPVARVFRSPE
                     YAEIDALIQRMGRGREGSLDREVHLRRGGQAVALLASATALRGPDGAYLGMVLAFDDL
                     TELLKAQRLAAWREVAQRIAHEIKNPLTPIQLSAQRLRRRLTTDRSPDEKRLLEEATA
                     TIIQEVDGLRQLVDEFSRFARMPALTPKPTDLARLLEGVVVLYRESHPSLAIRSAFSP
                     DLPSIEVDPDQIKRAVLNLVDNAVEAVGGTGEVCVQTAWLPEAHRVRIVVADDGPGIS
                     AEDRERLFMPYFSTKATGMGLGLPIVHQIVSDHGGTIRVEDNAPHGSRFVIELPAGRP
                     VAAPVQA"
     gene            6733..8097
                     /locus_tag="VFX87_15845"
     CDS             6733..8097
                     /locus_tag="VFX87_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4996454.1"
                     /translation="MPGEHILIVDDERAIQTTLRGVLEDEGYRVTAVGSGEQALARLQ
                     EDTPDIIFLDIWMPGLDGLDTLAEMKRLRPELAVVMISGHGTIETAVKATKLGAYDFI
                     EKPLSLEKTLLTATRALEHTRLERENRNLRAQLERGQEIVGKSPLIEHLRQQIAVAAP
                     SSGRVLIQGENGSGKELVARAIHTLSARREGPFVEVNCAAIPEELIESELFGHERGAF
                     TGAVARRRGKFELADQGTLFLDEIGDMSLKTQAKVLRVLEEQAFERVGGKDTIRVDVR
                     VLAASNQNLPERIAAGAFREDLYYRLNVIPIEVPPLRQRKEDLPLLVAHFIRLFSAEN
                     GKRPKAISVEALAYFLSYDWPGNVRELRNMVERLVIMSPRDVIGPEDLPPPLRPRDAE
                     AAADDGLRERTLKEAREAFERAFILSELRANEWNMTRTAERLGIERSHLYRKLKVYGI
                     SAPK"
     gene            8239..11610
                     /locus_tag="VFX87_15850"
     CDS             8239..11610
                     /locus_tag="VFX87_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4996455.1"
                     /translation="MQCLQCHADNQPHAKFCADCGAALSPACRRCASPLPPTARFCPE
                     CAEPTGARPTAGSRFASPHAYTPRHLADRMLSMRESLEGERKQVTVLFADLKSSLELL
                     ADRDPEEARQILDPILESMMEAVHHYEGTVNQVLGDGIMALFGAPLAHEDHALRACYA
                     ALRMQDSVRRYVAGIPDLAVPVRIRVGINSGEVVVRAIGGDLHMDYSAVGQTTHLAGR
                     MEQLAQPGATLLTRETLQLVQGHVTVRPTGPLLVKGLPDPLEAFELIGPGQARSRLQA
                     AAIRGLTRFVGREAELAMLHRTMERAGAGHGQVVCVIGEPGVGKSRLVHEFIHGGLPS
                     AWRVIETATVSYGRATPYLPIVALLRDYFQIEEGDDEVKVLDKVASRLRALDDALASG
                     IPVVLSLLDAPVADDAWDALDPTQRRRRTLEFLRRLFLRESQAQPLCLVLEDLHWIDS
                     ESQSLMDRVIESLPTARILCVVSSRPEFEHDWTNKSYYTQISLEPLAPGLAEALLDTL
                     LGGDPALDALKRQLIERTEGNPFFLEESVWHLAETGALAGARGAYHLARPIRDAQVPA
                     TVHAVLAARIDRLPALEKRLLQAAAVIGKDVPLPLLAAISDLGEDRLRLALRQLQSAE
                     LLYEVPRLPAPEYTFKHALTFEVAAASVLRERRRALDARIVETLEALGAEPRADQVER
                     LAHHALRGEVWDKALTYCRQAGARASARSAHRAAVEWFEHALAALAHLPETPHTAGAA
                     IDLRLDLRSSLSPLAEFTRMQDHLQEAERLAEAAGDRRRQGLVAAFLTNFYTVMLELR
                     RAIEYGQRAVAIAAQVGDVEAQVLAHTFLGLARYGQGDYRGAVELARHNVFLLRGDLV
                     RERFGMAVLPAVYSRTVLVWSLAELGEFADGLAIGQESARIAEAAEHPYSIVFTRFGL
                     GALHLRQGDLPRAAAELESGLALCRSADIRAPLLNVVTPLCSAYALSGRIADARKLVD
                     EAVDVAVTMGDPLGHWLRTGVLAEIHLAAGRAEDALPLARRAVELTRYVKSRGSEAWA
                     MRLLGEAEASQPVPLAEDAQATYRHALARALELEMRPLRARCHLGLGTLARRLGHDRE
                     AQAEIQIARDMFRALGMEYWSTRAESVLAADPAGG"
     gene            11701..12351
                     /locus_tag="VFX87_15855"
     CDS             11701..12351
                     /locus_tag="VFX87_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996456.1"
                     /translation="MSGNRPGGQNKLVITKKSLQYPVDPGNPGSVKIQKESAVDAQTP
                     KPVAPLQLKLGLRIKQAQTIEQFEVRFGPGPNGVLPLHVQPADDPIAQDLTAALGRGP
                     DERWAAYLADYDPAFGTTFTTKLRLSPFAVHPNGFQIVTVAFTHANHQFATDNYVFGL
                     SLLPGQNNAGTQLNLGGGGGGATSAKKPKPAAKVKAKASRKPSKAKKSAGAARRKR"
     gene            12618..>13109
                     /locus_tag="VFX87_15860"
     CDS             12618..>13109
                     /locus_tag="VFX87_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996457.1"
                     /translation="MSEVIINITADGRPLSGFNQYHYDIPDTLDPARVKIGPAEARQR
                     VARLAAPYRYRDIGKPRLMVHRYEPVGEEPPKPPRRRGGPRARFLAAVRAALQRRRRP
                     PIGTHLLVWDVRLSTERPRNRWRLLVDAMTGRLVEVRDQMAYARGRGSVYDPNPIVTS
                     GDVT"
BASE COUNT         1929 a         4810 c         4519 g         1851 t
ORIGIN      
        1 cggctcgaga cctgcgagac ctgccgcggg gccggcgtgg aggcgggcag ccagcccgag
       61 gtctgcggca cgtgccgcgg ccagggccag gtgcgcttca cccagggctt cctcacggtg
      121 gcccgcccct gccccaactg ccgcggcgag gggcgcatca tcaagcaccc gtgcccgggc
      181 tgccgtggcc aggggcgcgt atcccatgag cggctgctca aggtaaccat cccggccggc
      241 gtcgagaacg gcaaccagct ccggctcaac ggcgagggcg agagcgggct ccagggcggc
      301 ccggccgggg atctctacgt ggtgctgcac ctcaagcccc acgagatctt cgtgcgcgac
      361 ggcgcccacc tgatctgcga gctgccgctc accttccccc aggtggcgct cggcgatgag
      421 gtcgaggtgc cggtgctcga cggggccgca aagctgaaga tcccggcggg aacgcagccg
      481 ggccagcgcc tggtcctcaa gggcaagggc atgccccacc tgcgcgggcg gggccggggc
      541 gacgcggtgt acgaggtcgt cgtcgaggtg ccgacgcagc tcaccgtacg tcagcgcgag
      601 ctgctggagg agtttcgccg ctctcacgac ggcgactcga gcccccgggc ctccaagttc
      661 gtcgagcgga tgaagcagct attcggtagt tgatggcgtt ctggcagctc accctccccg
      721 cctccgccga cagcgaagac gcgctcacta atttcctgtg ggagcagggt gccctcggcg
      781 tggtcgagga agaggccccc ggcgtcccgc cgcgcctgcg cgccttcttc gccgagagca
      841 tgtcgtccac ccggctgctg gcggcggtcc aggactacca ggcgtcgctc cggtcgctcg
      901 gcttcgcgat cgaatcgcag ggcaccgaga tcacgccgct gctggacgaa gcctgggcca
      961 gcgcctggca gcagtcgttc ccggcccgcg aggtgggccg gcgcctcctc gtgctgccgc
     1021 cgtggatcgc gccgcccgtc cgcacggacg gggtgccccg cctgaccgtg atcatcgagc
     1081 cgggccgcgc cttcggcacc ggccaccacg gcaccaccga gggctgtctc gtgctgctcg
     1141 aggaagcgct ggcggccacg ccgggcgcgg ccacgctcga catcgggacc gggaccggca
     1201 tcctggccat cgcggcgctc aagctcggcg cgcccaccgt gctcgcgatc gacgtggatc
     1261 ccgacgccgt ctcggccacc caggtcaacg cggggcggaa cggctgcacc gggctgaccg
     1321 tccggctggc cgagccgcag gagatcccga accgcttccc gctcgtcctg gcgaacctgc
     1381 tgacgcacac ccacctggca ctcgccccgc agtacgcacg cctggtggcg cccggtggct
     1441 cgctggtgct gggcggcatg ctgcaggacg aggacgggcg cgtcagcgag gccctggccg
     1501 gcgccggctt cacgcctcga tcgcgcctgg tgctggaagg ctgggcctcg ctgcgcctcg
     1561 aggcaccgcc cgcgtgaccc gcttcctgca cctgggtcag gacgcggtgg acggcgatcg
     1621 ggtggccttc gaggccgcgg ccgctcatca cctgggccgc gtgctgcggg cggcggtcgg
     1681 ggacgtggtg caggcggtgg accacggggg cgcgctgctg tcggtgcgcc tcaccgcgat
     1741 cgcggcccgc cgcgccgagg gcctgatcgt gagccgtgcg ccgctcgcga cggagtcgcc
     1801 gctcgatctg accctggccc agggcgttcc gaagggcgac aagatggacg gcatcgtccg
     1861 gatggccacc gagcttggcg tctcccgcgt gatcccgctg ctgaccgcgc ggacggtggt
     1921 gcggctcgag cccgcgcgct ggacctcgcg cctcggccgc tggcagcgca tcgcaaagga
     1981 ggcggcccag cagagcgggc gcgcggccgt cccggagatc gcggcgcctc gcgacgtctc
     2041 gtcctgggct cgcgaggccg gcgcggccgg gctgctcgtc tgcctgtggg aagaggagcg
     2101 cgaagggctc gacaagcgtc tgccggcggg tccctgcccg cgcgtgaccg tggtcgtggg
     2161 cccggaaggt gggctgaccg ccgacgaggt gcgcgggctc accgacgcgg gcgccgtcgt
     2221 ggccgggctc ggccctcgcc tgctccggac cgagaccgcg ggcgcggtgg cggtggcact
     2281 gctccagtcg cgctacggcg atctcggcac cgcctgaagt gcagctcgcc gtcgaggccg
     2341 cgacgctgcc ggacgccttc gcgcggatcg cgctttcact cttcgccgtc gcggtcgatc
     2401 ccgcctcggt cgaccccgcg gaggtgcggg aggtgcgggc gcacggggcg ggcgtcctcg
     2461 cgctcctgcg ccgctggctc gaagaatgcc tctacgtcca cgaggtggag ggcttcgcct
     2521 gccggtctat cgagttcgtc gtcttcgagg cctcgcccac tgcgggcggc gagccgttcc
     2581 ggcttcatgc cttcctccgg ggtgagccgg tggaacccgc ccggcatcag gtgcacgggc
     2641 tgatccgggg tatcggaagc gatgggctca ccgtggagcc cttcgtggag ggcttccggg
     2701 tgcgggccag gctggagctt tgatgtcgcc tattggacac acgcgcccct tggggctgtg
     2761 tcattcttgc aacctcttac atagaactgg gcttgccgga tctctaaaaa ggcaatataa
     2821 gcgaatactt gcatgatcaa gtcccgcctg gcaccttgct tgcctattac ggcgctagac
     2881 atggactccc agcagaccgt cagacgcccg gtaagcctcg acggaatcgg tctgcattcg
     2941 ggcgagacgg cgaagatgac gatcgccccc gccgcggccg ataccgggat cctgttccgg
     3001 gccgccgacg gcacgctgat ccccgcgaac gccgatcacg tcgtagacac caattccgcg
     3061 accacggtcg gcgccttcgg cgtgcgggtg cgcaccatcg agcacctcat ggcggcggcg
     3121 gcggcgctcg gcatcgacaa cctgatggtg gacattgacg gacccgaagt gcccgcggcg
     3181 gacggcagcg ccaagccctt cgtggacctg ctgcgctccg cggggcgggt gagcttagca
     3241 gcccctcgcc gggcgatcac cgtcagcgag ccgatccgcg tgggcaccga gagccgctgg
     3301 atcgaggtcc tgccggcgga ctcactccgc atcagctaca ccctcgacaa caatcatccg
     3361 atcatcgggc tgcaggcggg cacgttcggg atcaccgaag aggtcttctg cgacgagctg
     3421 gccgccgcgc gcacgtacgg ctttctccga gacgtcccgg ccatgaggca gaacggcctg
     3481 gcccgcgggg gctcgctcga caacgcgatc gtggtcggca agcgctcggt gctcaacgat
     3541 agcctgcgct tccctgacga gttcgtccgc cacaagatcc tcgacctggt gggggacctg
     3601 ttcctcctgg ggcggccgct ccgggcgcac gtggtgggac gcaatgccgg ccacgccctc
     3661 aactaccagg tggtggccgc gatccagaag gccctggcgg ccgaccgccg gcgcgttgcc
     3721 gcacgggccg cccgccccgt ccccgccccg gtggccgcca gcgacgggtt cctcccgggt
     3781 gtcgcggccc tgtaggccgc cgcccgctcc gctccccctc ttcccgaccg cccggagcgc
     3841 ccaaggccga accgccccgc ccgtagggcc cgacgctata atggtccggc tgctctatga
     3901 ggacttaccg ccgccgtctg atccgaacgt ttgccctggc cagcggtctg atcgccgtcc
     3961 tgctgggcgc tccggccgcg gaggccgagg tccggatcag caacctggcg gtcttcctga
     4021 acgactacga cgtcaccgtc acggtggtcc tgttcggggc ggtgccggac gcactccatg
     4081 agagcatcca caccggcatc gccgcccatg tgcggtacta cgtcgagctc tggcagcaca
     4141 cccgcatggg cgtggaccgg cgcctgcagg cgcgcatggt ggagcgccaa ctcacctaca
     4201 acgtgctcac caaggagtac aaggtcgcgc cgctcaaggg cgagcagcgc gagccgctgc
     4261 tcaccaagga cctacgcgag gcccagcgcg tgatctccga gctccgggtg ggccagctgg
     4321 cgccagtcgc cgcgctggac aagcaggagc tctactacgt ccgggtgcgc tccgacgtct
     4381 ccctgggcgg cgtcaactcc tggttcgcgc gaatgaccgg tgaggccgag gagacaccgt
     4441 gggtgcagtc gagcctcctc accccgatgc ggagccagtg atgccgctca cgccgcggcc
     4501 ttcgccggtg gacgagcagg agaagcgcaa gcggaacctc ctcatcatca cggggatcct
     4561 ggtcctcctg gtggtggcga ccgtcttcga ggtgggcatc cgcgcgcccc agatcccgtt
     4621 cgcctccaac ctcatcgtct tcttcctctt caacctcaac ctgatcgtct tcctgctgct
     4681 gctggtgctg ctcttccgca atttggtcaa gctctccttc gagcgacggc agaagatcat
     4741 cggctccaag ttcaaggcca agctcgtcct cacctttctg gccctggcgc tggtgcccgg
     4801 catcctgatc ttcctcatcg cctcgaactt catcaccacc tccatcgagg gctggttcaa
     4861 gccgcaggtc gagaagccgc tcgaccaggc catggaggtg gcgcagacct actaccagag
     4921 cctcgagacc accgcgctgc gccacggccg ctacatgggc cgcgtgatcg agcgcgacgg
     4981 gctcctcgcc gagagcaagc gcgaggcgct ggccagctac ctggccgagc agcaggaccg
     5041 cctcggcgtc gccgccatca ccgtcttcaa ccgggacggg caggagctgg tgcacgtcaa
     5101 gaacccggtg ctcgccaagg tgcccacccg cgaggccaac agcgagcacg tgcggcaggc
     5161 gctctcgggc caggagatca ccaccgtcca cgagctggac aacggcgaca tgatccaggc
     5221 catggtgccg gtgcgcgacg gcgaccaggc ggtgcccgcc acgatcgtgg tggccatcca
     5281 cgtgccccag cgcctcgagg cgcggctgcg caatatcagc caggccttcc aggagtacaa
     5341 gcagctccgc ctgctcaaga acccgatcaa gggcatctac atcctgctct tcctgctgat
     5401 gaccctgatc atcgtcttct ccgccacctg gttcggcctc tacctggccc gcggcatcac
     5461 ggagccaatc cagatgctgg ccgaaggcac tcgcgaagtc gcggcgggca acctcaacta
     5521 caaggtgacg gtccgggccg acgacgagat cggcatcctg gtggactcct tcaaccggat
     5581 gaccggcgac ctcgccgcgt cgcaatcgaa gctggaggag acctacaagg acctccaggc
     5641 caagcacggc gaggtggaag atcgccgccg ctataccgag accgtgctgg aggcggtcgc
     5701 caccggcgtg gtgtcgctcg acgcggaggg gcaggtcacc acgatcaacg gcgccgccga
     5761 gcgcctgctc ggcctgaccg ccgcccagat ccagggccgg cccgtcgcgc gggtgttccg
     5821 ctccccggag tacgcggaga tcgacgcgct gatccagcgc atgggccgcg ggcgcgaggg
     5881 ctcgctggac cgcgaggtgc atctccgccg cggcggccag gcggtggcct tgctcgcctc
     5941 cgccaccgcg ctgcgcggac ccgacggcgc ctacctgggc atggtgctgg ccttcgacga
     6001 tctcaccgag ctgctgaagg cgcagcgcct ggccgcctgg cgcgaggtcg cccagcgcat
     6061 cgcgcacgag atcaagaacc cgctgacccc catccagctc tccgcccagc gcctccggcg
     6121 ccgtctcacc accgaccgga gccccgacga gaagcggctg ctcgaggaag ccaccgccac
     6181 catcatccag gaggtggatg ggctccgcca gctcgtggac gagttctcgc gcttcgcccg
     6241 gatgcccgcg ctcaccccca agcccaccga tctggcgcgg ctgctcgagg gcgtggtggt
     6301 gctctaccgc gagtcccatc cgagcctggc catccgctcc gccttctccc ccgacctgcc
     6361 ctcgatcgag gtggatccgg accagatcaa gcgcgcggtc ctcaacctgg tggacaacgc
     6421 ggtggaggcg gtgggcggca ccggcgaggt ctgcgtccag accgcgtggc tgcccgaggc
     6481 gcaccgggtc cggatcgtgg tcgccgacga cgggccgggc atctcggccg aggaccgcga
     6541 gcggctcttc atgccctact tctccaccaa ggccaccggg atggggctcg ggctgccgat
     6601 cgtccaccag atcgtgtccg accatggcgg gacgattcgg gtggaagaca acgcgccgca
     6661 cggcagccgc ttcgtgatcg agctgcccgc cggccggccg gtggccgcgc ccgtgcaggc
     6721 ctgagccgac ccatgcccgg cgagcacatc ctgatcgtgg acgacgagcg ggcgatccag
     6781 acgacgctca gaggcgtcct ggaggatgaa ggatatcgag tgaccgcggt gggctcgggc
     6841 gagcaggccc tcgcgcgcct gcaggaggac acgcccgaca tcatcttcct cgacatctgg
     6901 atgccgggcc tcgacggcct cgacacgctg gccgagatga agcggctgcg gccggaactc
     6961 gcggtggtga tgatctcggg ccacggcacc atcgagaccg cggtcaaggc caccaagctc
     7021 ggcgcctacg acttcatcga gaagccgctc tcgctcgaga agacgctgct caccgccacc
     7081 cgggccctcg agcacacgcg cctcgagcgc gagaaccgca acctgcgcgc ccagctcgag
     7141 cggggccagg agatcgtggg caagagcccg ctgatcgagc acctgcgcca gcagatcgcg
     7201 gtcgctgcgc cctcgagcgg ccgcgtgctc atccagggcg agaacggctc cggcaaggag
     7261 ctggtggccc gcgccatcca cacgctgtcc gcgcggcgcg aggggccctt cgtggaggtc
     7321 aactgcgcgg ccatccccga ggagctgatc gagtccgagc tgttcggtca cgagcgcggc
     7381 gccttcaccg gggcggtggc gcggcggcgc ggcaagttcg agctggccga ccagggcacg
     7441 ctcttcctcg acgagatcgg cgacatgagc ctcaagaccc aggccaaggt gctgcgggtg
     7501 ctggaggagc aggccttcga gcgcgtgggg ggcaaggaca cgatccgggt ggacgtgcgg
     7561 gtgctggccg cctccaacca gaacctgccc gagcgcatcg ccgcgggtgc cttccgggaa
     7621 gacctctact atcgtctgaa cgtgatcccg atcgaggtgc cgccgctgcg gcagcgcaag
     7681 gaagacctgc cgctgctggt cgcccatttc atcaggctct tctcggcgga gaacggcaag
     7741 cggcccaagg ccatctcggt cgaggccctc gcctacttcc tctcgtacga ctggccgggc
     7801 aacgtgcgcg agctgcgcaa catggtcgag cggctcgtca tcatgagccc gcgcgacgtg
     7861 atcggccccg aggacctccc gcccccgctc cgcccgcggg acgcggaggc ggcggccgac
     7921 gacggcctgc gcgagcgcac cctcaaggag gcgcgcgagg ccttcgagcg cgccttcatc
     7981 ctgagcgagc tgcgggccaa cgaatggaac atgacccgca ccgccgagcg cctcggtatc
     8041 gagcgcagcc acctctaccg gaagctgaag gtctacggga tcagcgcgcc gaagtagcga
     8101 ccggaggacc gtgctccacg tcgcgtgagc cccaggagct cccagtcggt ggcggagccc
     8161 ggacgcgagc cttgaccccc gacccgggaa atactacggt gcgctagtcc ctccagcgtc
     8221 gggacgaggg cccacgagat gcagtgtctc cagtgtcacg cggataacca gccccacgcg
     8281 aagttctgcg cggattgcgg cgcggcgctg tcgcccgcgt gccgccggtg cgccagcccg
     8341 ctgccgccga ccgcgcgctt ctgtccggag tgcgcggagc ccaccggggc gcggccgacc
     8401 gcaggctccc gcttcgcctc accgcacgcc tatacgcccc ggcacctggc ggaccgcatg
     8461 ctgagcatgc gcgagtcgct cgagggcgag cgcaagcagg tcacggtgct cttcgcggac
     8521 ctgaagagct cgctggagct cctcgccgat cgggatccgg aggaggcgcg gcagatcctc
     8581 gacccgatcc tcgaatcgat gatggaagcg gttcaccact acgagggcac cgtcaaccag
     8641 gtgctcggcg acgggatcat ggccctcttc ggcgcgccgc tggcccacga ggatcacgcg
     8701 ctccgcgcct gctacgcggc gctgcgcatg caggactcgg tccggcgcta cgtggccggg
     8761 atcccggacc tggcggtccc ggtgcgcatc cgggtcggga tcaactcggg cgaggttgtg
     8821 gtgcgcgcca tcggcggcga cctgcacatg gactactccg cggtcgggca gaccacgcat
     8881 ctcgccgggc gcatggagca gctcgcgcag ccgggcgcca ccctgctcac gcgcgagacg
     8941 ctgcagctcg tgcagggcca cgtcaccgtg cggccgaccg ggcccctgct ggtcaagggc
     9001 ctccccgacc cgctcgaggc cttcgagctg atcgggccgg gccaggcgcg ctcccggctc
     9061 caggccgccg cgatccgggg actcacgcgc ttcgtcggcc gcgaggccga gctcgccatg
     9121 ctccaccgca cgatggagcg cgccggcgcc ggtcacgggc aggtcgtctg cgtcatcggc
     9181 gagccgggcg tcggcaagtc gcgcctcgtc cacgagttca tccacggcgg gctgccgtcc
     9241 gcgtggcggg tgatcgagac ggccaccgta tcgtacggtc gggcgacgcc gtaccttccg
     9301 atcgtcgcgc tgctacgcga ctacttccag atcgaggaag gcgacgacga ggtcaaggtg
     9361 ctcgacaagg tcgcctcccg gctccgcgcg ctcgacgacg cgctggcctc ggggatcccg
     9421 gtggtgctct cgctcctgga cgcgcccgtc gccgacgacg cctgggacgc gctcgatccg
     9481 acccaacggc ggcggcgcac gttggagttc ctgaggcgcc tcttcctgcg ggagagccag
     9541 gcccagcccc tctgcctcgt gctggaggac ctgcactgga tcgattcgga atcgcagtcc
     9601 ctgatggacc gggtgatcga gagcctgccc accgcgcgga tcctgtgcgt ggtcagctcg
     9661 cgacccgagt tcgagcacga ctggaccaac aagagctact acacccagat ctcgctggag
     9721 ccgctcgccc ccgggcttgc cgaggcgctg ctggacacgc tgctgggcgg cgaccccgcg
     9781 ctcgacgcgc tcaagaggca gctcatcgag cgcacggagg gcaacccgtt cttcctggag
     9841 gagagcgtct ggcatctcgc ggagacgggc gcgctcgccg gcgcgcgcgg cgcctaccac
     9901 ctcgcccggc cgatccgcga cgcgcaggtg ccggccaccg tccacgcggt cctggccgcc
     9961 cgcatcgacc ggctccccgc gctggagaag cggctcctgc aggccgccgc ggtcatcggc
    10021 aaggacgtgc ccctgccgct gctcgccgcg atcagcgacc tcggggaaga ccggctgcgg
    10081 ctcgccctgc ggcagctcca gtccgccgaa ctgctctacg aggtgccgcg gctccccgcg
    10141 cccgagtaca cgttcaagca cgcgctcacc ttcgaggtgg ccgcggccag cgtgctgcgg
    10201 gagcggcgcc gcgccctcga cgcgcgcatc gtggagacgc tcgaggcgct cggcgccgag
    10261 ccccgcgccg atcaggtcga gcgcctcgcg caccatgccc tccgcggcga ggtctgggac
    10321 aaggcgctga cctattgccg ccaggccggc gcgcgcgcct ccgcgcggtc ggcccaccgc
    10381 gcggcggtgg agtggttcga gcacgcgctc gccgcactcg cgcacctgcc ggagacgccg
    10441 cacaccgcgg gggcggcgat cgacctgcgc ctcgacctgc gctcgtcgct gagcccgctg
    10501 gccgagttca cccggatgca ggaccacctg caggaggccg agcggcttgc ggaggccgcg
    10561 ggcgaccggc gccggcaggg gctcgtggcc gcgttcctga cgaacttcta caccgtgatg
    10621 ctcgagctcc ggcgggcgat cgagtacggg cagcgcgcgg tcgccatcgc ggcccaggta
    10681 ggcgacgtcg aggcgcaggt cctggcccat accttcctgg gcctcgcgcg ctacggccag
    10741 ggtgactacc gcggggcggt ggagctggcg cgccacaacg tgttcctcct gcgcggcgac
    10801 ctcgtccggg agcgcttcgg catggcggtc ctgcccgcgg tctactcgcg caccgtgctc
    10861 gtgtggagcc tggccgagct gggcgagttc gccgacggcc tcgcgatcgg gcaggagagc
    10921 gcgcggatcg cggaggcggc cgagcatccc tacagcatcg tcttcacccg cttcggcctg
    10981 ggcgccctgc acctccgcca gggcgatctc cctcgcgcgg cggccgaact cgagagcggc
    11041 ctcgcgctgt gccgcagcgc ggacatccgc gccccgctcc tgaacgtggt cacgccgctc
    11101 tgctcggcct acgccctgtc cgggcggatc gccgacgcgc ggaagctggt ggacgaggcg
    11161 gtggacgtgg cggtgaccat gggcgatccc ctcgggcact ggctccggac gggcgtcctc
    11221 gccgagatcc acctcgcggc cggacgggcc gaggacgcgc tgcccctggc ccggcgcgcg
    11281 gtcgaactga cccgctacgt caagagccgc ggatcggaag cctgggcgat gcggctcctc
    11341 ggcgaggccg aggcgagcca gcccgtcccg ctcgccgagg acgcgcaggc cacctatcgc
    11401 catgccctcg cccgggcgct cgagctcgag atgcgcccgc tccgggcacg gtgccacctc
    11461 gggctcggga ccctcgcgcg gcgactcggg cacgaccgcg aggcgcaggc cgagatccag
    11521 atcgcccggg acatgttccg cgcgctcggc atggagtact ggtcgacccg cgcggaatcg
    11581 gtgctggccg cggaccccgc gggaggctga agccgggctg tcttgcggtg ccgtcgtccc
    11641 gcgtgctagc gtccccggga tagatccgcg accacttcga cccgaaggag ggcgcacgcc
    11701 atgtcaggga acaggcccgg cggtcagaac aagctggtca tcacgaagaa gagtctccag
    11761 tatccggtcg atcccggcaa tccgggcagc gtcaagatcc agaaggagag cgcggtcgac
    11821 gcgcagacgc ccaagcccgt cgcacccctc cagctcaagc tgggcctgcg catcaagcag
    11881 gcccagacca tcgagcagtt cgaggtgcgc ttcgggcccg gccccaacgg tgtcctgccg
    11941 ctccacgtcc agcccgccga cgatcccatc gcgcaggact tgacggccgc gctcggtcga
    12001 ggtccggacg agcggtgggc cgcctacctg gccgactacg atccggcctt cgggaccacg
    12061 ttcacgacga agctccggct gagccccttc gccgtgcatc ccaatggctt ccagatcgtc
    12121 acggtggcgt tcacccacgc caaccaccag ttcgccaccg ataactacgt gttcggcctc
    12181 agcctgctgc ccgggcagaa caacgccgga acccagctca acctcggagg gggcggtggc
    12241 ggggcaacgt ccgccaagaa gccgaagccc gccgccaagg tgaaggccaa ggcctcgcgg
    12301 aagccctcga aggccaagaa gtccgcgggc gcggcgcgac ggaagcggta gaccgcaagc
    12361 gaccgatccg gcgcgacggc cgagtcatcg tcgacccggt caacggcgct ctgcggagct
    12421 ggctcgagcc ggccgcccaa cccgaggcgc gtcctccgtt ccctccgcgc gaggtcgcgc
    12481 ggcgcctcct gcaggaaaac gccgcgctct tcgcttggcc ttcgagcctc ttcgacctgc
    12541 gggacgccat cctccacgag cggccggacg gatggtcggt acgcctgacc caggacttca
    12601 agggcgtacc cgtcgacgtg agcgaagtga tcatcaacat caccgccgac ggacggcccc
    12661 tatccgggtt caaccagtac cactacgaca tccccgacac gctcgatccc gcccgggtca
    12721 agatcgggcc cgccgaggcg cgccagcggg tcgcccggct cgccgctccg taccgctacc
    12781 gtgacatcgg caagccgagg ctcatggtcc accgctacga accggtggga gaggaaccgc
    12841 cgaagccccc acggcgccgc ggcggacccc gggcgcggtt cctggccgcg gtccgtgccg
    12901 cgctccagcg cagacgccgg ccgccgatcg gcacgcacct gctggtctgg gacgtccggc
    12961 tctccaccga gaggcccagg aatcgttggc ggctcttggt cgacgcgatg accggccggc
    13021 tggtcgaggt gcgcgaccag atggcctacg cgcggggtcg tggctccgtg tacgatccga
    13081 acccgatcgt gaccagtgga gacgtgacc
//
LOCUS       DASUFA010000436         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_201647, whole genome shotgun sequence.
ACCESSION   DASUFA010000436 DASUFA010000000
VERSION     DASUFA010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201647"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFX87_15865"
     CDS             <1..1067
                     /locus_tag="VFX87_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4996458.1"
                     /translation="EWEEAGQIPKSIWPRMGALGFLGVEYDEKYGGAGADVLTTAVLC
                     EEMARARCASLAMAVGVHTDMASPHLYWTGSEALKERYLPAICRGEALTAIAVTEPGG
                     GSDVAAIRTRAVRDGDHYVLDGSKMFITNGVLADLYFVAARIEQGTGDRRHRGISMFL
                     VERNTPGFTVSRKLEKMGNRASDTAELAFEGMRVPAGNLLGTEGKGFYEVMRVFQRER
                     LVAGLHAVAGCERALEDTIAYVQQRHAFDEPLSKKQVVRHRLADLATLIEAGRWLTYA
                     ACLKFRDREEAVKEISMVKLFTAEMAQKVAYECVQLHGGYGYMREYPIERFFRDIRLM
                     TIGGGTSEIMKEIIAKQMAI"
     gene            1064..1927
                     /locus_tag="VFX87_15870"
     CDS             1064..1927
                     /locus_tag="VFX87_15870"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX4996459.1"
                     /translation="MTSVDFVAIGHVTLDQTPRGTRPGGAAYYTAMTAHRLGLRVGLL
                     SSFGADFPREAIPAGIEVVNVPAARTTTFRIGQSRVGQSGRTRELAVLARAADLEADA
                     LPEEWRDATLGALAPVTGEVDPGFAAAFPETSLVALPQGWMRQRGKGGILAPQPWEDA
                     DLVLPRVQAVVVSHEDVEPFEKDAFEWFQQVPIGAVTRGPAGAILFVNGERYGVEADR
                     AVAVDETGAGDVFAAALLIAYNRDGNAWDAAAAAACCAAASVEAEGAAGIPDRAGLEA
                     RLAAYRRRHGI"
     gene            1995..>2218
                     /locus_tag="VFX87_15875"
     CDS             1995..>2218
                     /locus_tag="VFX87_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEX4996460.1"
                     /translation="MAERKIRITAGRVTADAVLNTSKTASAVWDALPLDIPGETWGDE
                     IYFSIPVKAKGEDAREVVDLGDLAYWPPGSA"
BASE COUNT          321 a          756 c          834 g          307 t
ORIGIN      
        1 aggagtggga agaggccggt cagatcccca agtcgatctg gccccgcatg ggcgcgctgg
       61 gcttcctggg ggtggagtac gacgagaagt acggcggcgc gggtgccgac gtcctcacca
      121 ccgcggtgct ctgcgaggag atggcccggg cccgctgcgc ttcgctggcc atggcggtcg
      181 gcgtgcacac cgacatggcc tcgccccatc tctactggac ggggagcgag gcgctcaagg
      241 agcggtacct gcccgcgatc tgccgcggcg aggccctcac cgcgatcgcg gtgaccgagc
      301 cgggcggcgg ctccgacgtg gccgcgatcc gcacccgcgc ggtgcgcgac ggcgaccact
      361 acgtgctcga cggctccaag atgttcatca ccaacggcgt gctggccgat ctctacttcg
      421 tggccgcgcg catcgagcag ggaaccgggg accggcggca tcgcgggatc tcgatgttcc
      481 tggtggagcg gaacaccccc ggcttcacgg tgagccggaa actcgagaag atgggcaacc
      541 gcgcctccga caccgccgag ctcgccttcg agggcatgcg ggtgcccgcc gggaaccttc
      601 tcggcaccga gggcaagggt ttctacgagg tcatgcgcgt gttccagcgt gagcgcctgg
      661 tggcgggcct gcacgcggtg gccggctgcg agcgggcgct ggaagacacc atcgcctacg
      721 tccagcagcg ccacgccttc gacgagccgc tctcgaagaa acaggtggtg cggcaccggc
      781 tcgccgacct cgccaccctc atcgaggccg ggcgctggct cacctacgcg gcgtgtctca
      841 agttccggga ccgggaggag gcggtcaagg agatctcgat ggtgaagctc ttcactgccg
      901 agatggcgca gaaggtcgcc tacgagtgcg tgcagcttca cggcggctac ggctacatgc
      961 gcgagtaccc catcgagcgc ttcttccggg acatccgcct catgaccatc ggcggcggga
     1021 cctccgagat catgaaggag atcatcgcca agcagatggc gatatgacgt cggtcgactt
     1081 cgtcgcgatc gggcatgtca cgctcgacca gacgccgcgg ggcacgcggc ccgggggcgc
     1141 ggcgtattac acggcgatga cggcgcaccg cctgggcctt cgcgtgggcc tgctcagctc
     1201 cttcggggcc gattttccgc gggaggcgat tcccgccggc atcgaggtgg tgaacgttcc
     1261 cgccgcgcgg accacgacgt ttcgtatcgg gcagagccgc gtcggccaga gcgggcggac
     1321 acgggagctc gcggtgctgg cccgggcggc cgatctggag gcggacgcgc tgcccgagga
     1381 gtggcgggac gcgacgctgg gcgcgctggc tccggtcacc ggcgaggtgg acccgggctt
     1441 cgccgccgcg ttcccggaga cctcgctggt ggccctgccg cagggctgga tgcgccagcg
     1501 cggcaagggc ggcatcctgg cgccccagcc ctgggaggac gccgacctgg tgctgccgcg
     1561 cgtgcaggcg gtggtggtca gccacgagga cgtcgagccg ttcgagaagg acgcgttcga
     1621 atggttccag caggtgccga tcggcgcggt cacccgcggg ccggccgggg cgatcctctt
     1681 cgtcaacggc gagcggtacg gcgtcgaggc ggatcgcgcg gtggcggtgg acgagaccgg
     1741 cgccggagac gtcttcgcgg ccgcgctgct catcgcctac aaccgcgacg gcaacgcgtg
     1801 ggacgcggcc gccgcggccg cctgctgcgc cgccgcgtcc gtcgaggccg aaggcgccgc
     1861 gggtatcccg gaccgcgccg gcctcgaggc gcggctcgcc gcctatcggc gccggcacgg
     1921 catctgagcg cgggcttcgc cttgccgccg gccgcggccc ggggctacac tcggagcggg
     1981 gcggacgaat ccacatggcg gagcggaaga tccggatcac cgcgggccgc gtcacggccg
     2041 acgcggtgct gaacacctcg aagacggcct cggcggtctg ggacgcgctg ccgctcgaca
     2101 ttcccgggga gacctggggc gacgagatct acttcagcat ccccgtgaag gccaaggggg
     2161 aggacgcgcg cgaggtggtg gatctgggcg acctcgccta ctggcccccg ggctccgc
//
LOCUS       DASUFA010000437         6039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_673524, whole genome shotgun sequence.
ACCESSION   DASUFA010000437 DASUFA010000000
VERSION     DASUFA010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673524"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="VFX87_15880"
     CDS             complement(<1..1640)
                     /locus_tag="VFX87_15880"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4996461.1"
                     /translation="MSRLRVGVDVGGTFTDVVSVDDVTGEVRSCKVATVPDNRAQGVL
                     DGLRALLRAGEACASLAHGTTTVTNAIVEGRVAPVGLVTTRGFRDVLEIARMNRAHLY
                     RLELPARAEPLVPRRRRLEITERVGPDGTVQTPVAPGELEGIVAALREEGVEAVAVCL
                     LHAYANPAHEQALRRALEPHFRHVSISSEINAEFREYERTCTTALNASVMPLADRYLG
                     GLTAALGDAAGGPALRLLHSAGGMMSVAAARARPLAMALSGPAAGVAAAAHVARTLGI
                     DRALAFDMGGTTTDVCLISDGAAETTSQRKLADYPVRLPMVAVESIGAGGGSLARVDE
                     VGALKVGPQSAGAVPGPACYGQGGTLATVTDANLVLGYLNPARLYGGTIRIHPDRARS
                     AVEAVGRRLGLSMLEAAHGIVEVANASMLRALRLVSVQRGHDLRRFSLIAYGGAGPVH
                     AGALARACGIGRVIVPAHSGAFSALGCLVSPLRYDAVQTHRARLDAWDGKLIEDRFRA
                     LEEQCLRPILEEGHAASAVTVQRSLDVRYVGQNYEIELP"
     gene            complement(1660..2925)
                     /locus_tag="VFX87_15885"
     CDS             complement(1660..2925)
                     /locus_tag="VFX87_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996462.1"
                     /translation="MRRRDVVKLLGMGALAASAPRAWAQGREVVIGALYPLSGPVAQA
                     GVDAKAAAETAAEIVNTRQDLDLPLARTEGLPGLGGAKLRLVFVDHQGKPELGQSEAE
                     RLITQEKAVALYGAYHSSVSATASVVCERLGIPYLSGESSSPTLHRRGFKWFFRTSPH
                     DEHFSIAMFEFLKELQGRSGKKIATLGIIHEDTLFGADSARVQEQLAQAGGYRVVEKL
                     AYRARTTSMQTEVQRLKAANPDVLLPTSYTSDAILFIKTARELDYNPPMILAQDAGYV
                     ESDFVAGVGKDADGVMSRSVFSLDLAAKRPVIGRVNEMYKKRSGKDLNDNTSRAFTGI
                     MVLAEAINRARSTDPEAIRKALAATDLKPNQLIMPWKGVTFDPKTGQNELGTPLITQW
                     RGGALKIAWPFDLAAGDLMYPLPKWAERK"
     gene            3202..4044
                     /locus_tag="VFX87_15890"
     CDS             3202..4044
                     /locus_tag="VFX87_15890"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX4996463.1"
                     /translation="MAETVTWPDGARCAIMMTFDLDGESPWIHRDPGLAERPLHMWMG
                     AYGPKTGMPRILELLDRYGIKTCIFIPGWIVERYPALCEDILKHGHEVGHHGYLHEKP
                     FFMMSREEEEALLVKSLEIFKKVLGVVPLGSRTPSADPSRHTMELLARHGFLYHSNAL
                     DTDLPYCHETVHGPLVEFPTAWCNNDAPFFLWSAVPPVGNGIWSQQDVWEIWSEEFEG
                     LYAEGGFFNWLAHPQIIGRPARLRMVERLIQLILGKGRVWWPRPVDLARFWLERRKTP
                     VASA"
     gene            4044..4808
                     /locus_tag="VFX87_15895"
     CDS             4044..4808
                     /locus_tag="VFX87_15895"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4996464.1"
                     /translation="MAGGRLAGKVALITGAGRGFGRAIAQAFAREGARVAANYLGSRA
                     GAEAVVAEAERLGAEAVALRGDVAREDDVKALVAATLARFGRLDVLVNNAGIMVRAPM
                     LAVPAEECRRMFDVNVTGTMLCSRYALPAMIERKGGRIINLSSQLAQRPLGGGGFAAY
                     AATKGAIEALTRALAAEVGAHGITVNAIAPGGIETDMSRDVMTEEYRTRRLGELPLRR
                     FGRVEDVAYCAVVLASDEAGYLTGQILHPSGGWVSG"
     gene            4805..>6039
                     /locus_tag="VFX87_15900"
     CDS             4805..>6039
                     /locus_tag="VFX87_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX4996465.1"
                     /translation="MTAVRYPWPPGRKSAAVLSFDFDAESGFLFREPEKARRSLGDLE
                     ERRFGPRVGVDRILRLLDRLRLRATFFIPGWTVEHHFEACRRIRDAGHEIGAHGNVHE
                     ALGFLDEEQETRVMREQLEILASRLGVRPAGYRSPSWDVNVWTPDLLKRHGFLYDSSL
                     MGNDVPYDVPTSQGPLTEVPVQWLLDDAPLFRHVYGTTNAIADPGRVLQMWSKEFAAM
                     HAESGCFVLTCHPFVSGRASRIALLDDLVTFMRRARGVWFTTCEEIARWHAAGIRDGV
                     GGPVHVTEEGRVVEQPLHRHLDQRSLAGVDLVGHVVAHQRAVVEEAVPLEQRRRPHVD
                     VPRRRAVAGRAHAEARLQDVELLAHHPLFLRLVEEAQRLVHVAVRADLVAGVADARGF
                     GRVVLHRPSRDEEGRPELQP"
BASE COUNT          824 a         2195 c         2144 g          876 t
ORIGIN      
        1 ggcagctcga tctcgtagtt ctgcccgaca tatcgaacat ccaggctacg ctgcacggtc
       61 accgcggagg cggcgtggcc ttcctcgagg atcggccgca ggcactgctc ctcgagggcc
      121 cggaagcggt cttcgatcag cttgccgtcc caggcgtcga gccgggcgcg gtgggtctgc
      181 accgcgtcgt agcgcagcgg cgagaccagg cagccgagcg cggagaaggc gcccgagtgc
      241 gcgggcacga tgacgcgccc gatgccgcac gcccgcgcga gtgcgcccgc gtgcaccggg
      301 cccgcgccgc cgtaggcgat gagcgagaac cggcgcaggt cgtggccgcg ctgcaccgag
      361 accaggcgca gcgcgcgcag catgctcgcg ttcgcgacct ccacgatgcc gtgcgcggcc
      421 tcgagcatgg agaggccgag ccgccggccg accgcctcca ccgcgctccg ggcgcggtcg
      481 gggtggatac gaatagtccc gccgtagagc cgggccgggt tcaggtagcc gagcacgagg
      541 ttggcgtcgg tcacggtggc cagcgtcccg ccctggccgt agcaggccgg gccggggacg
      601 gcgcccgcgc tctgcggccc caccttgagc gcgccgacct cgtccacgcg cgcgagcgag
      661 ccgccgcccg cgccgatgga ctccaccgcc accatgggca gccgcaccgg gtagtcggcc
      721 agctttcgct gcgaggtggt ctcggccgcg ccgtcgctga tcaggcacac gtcggtggtg
      781 gtgccgccca tgtcgaaggc cagggcgcga tcgatcccga gcgtgcgcgc cacgtgggcg
      841 gccgcggcca cgcccgcggc ggggccggag agcgccatcg cgagcgggcg cgcccgggcc
      901 gcggccaccg acatcatgcc gcccgccgag tgcagcaggc gcagcgcagg gccgcccgcg
      961 gcgtcgccga gcgcggcggt gagcccgccc aggtaccgat cggcgagcgg catcacgctg
     1021 gcgttcagcg cggtggtgca ggtgcgctcg tactcgcgga actccgcgtt gatctcgctc
     1081 gagatcgaca cgtgtcggaa gtgcggctcg agcgcccgcc ggagcgcctg ctcgtgcgcc
     1141 ggattggcgt aggcgtggag caggcagacc gcgaccgcct ccaccccctc ctcccgcagg
     1201 gcggcgacga taccctcgag ctcgccggga gccaccgggg tctgcacggt gccgtccggc
     1261 cccacccgct cggtgatctc gaggcgccgg cggcgcggca ccagcggctc cgcccgtgcg
     1321 ggcagctcga ggcgatagag atgcgcgcgg ttcatccgcg cgatctcgag cacgtcgcgg
     1381 aagccgcgcg tggtgacgag gcccaccggg gcgacgcgcc cctccacgat ggcattggtg
     1441 acggtggtgg tgccgtgggc gagcgaggcg caggcctcgc ccgcccgcag cagcgcgcgc
     1501 aggccgtcga gcacgccctg agcgcggttg tcgggcaccg tggccacctt gcacgagcgc
     1561 acctccccgg tgacgtcgtc cacggagacg acgtcggtga aggtgccccc cacgtccacg
     1621 ccgacgcgga ggcggctcac acccggatcg aggcgtccgt cacttgcgct cagcccactt
     1681 cggcagcggg tacatcaggt cgcccgcggc caggtcgaac ggccaggcga tcttgagcgc
     1741 tccgccccgc cactgggtga tcagcggggt gcccagctcg ttctggccgg tcttggggtc
     1801 gaaggtcacg cccttccacg gcatgatgag ctggttcggc ttgagatcgg tagcggccag
     1861 cgccttgcgg atcgcctcgg ggtcggtgga gcgcgcccgg ttgatcgcct ccgcgagcac
     1921 catgatgccg gtgaaggcgc gcgaggtgtt gtcgttgagg tccttcccgg atcgcttctt
     1981 gtacatctcg ttgacccggc cgatcaccgg ccgcttggcg gccaggtcca gcgagaacac
     2041 ggagcgggac atgaccccgt cggcgtcctt gcccaccccg gccacgaagt cgctctccac
     2101 gtagcccgcg tcctgggcca ggatcatcgg cgggttgtag tcgagctcgc gcgcggtctt
     2161 gatgaacagg atcgcgtcgg acgtgtagga ggtcggcagc aggacatccg gattcgccgc
     2221 cttgagccgc tgcacctcgg tctgcatgct ggtggtgcgc gcgcggtagg ccagcttctc
     2281 caccacccgg tagccgccgg cctgggcgag ctgctcctgc acccgcgccg agtccgcgcc
     2341 gaagagcgtg tcctcgtgga tgatgccgag cgtggcgatc ttcttcccgc tccggccttg
     2401 cagctccttc aggaactcga acatcgcgat cgagaagtgc tcgtcgtgcg ggctggtgcg
     2461 gaagaaccac ttgaagccgc ggcggtggag cgtgggcgag gacgactcgc ccgagaggta
     2521 cgggatgccg aggcgctcgc acaccacgct cgcggtggcc gacacgctgc tgtggtaggc
     2581 cccgtagagc gcgaccgcct tctcctgcgt gatcagccgc tccgcctccg actgtcccag
     2641 ctccggcttg ccctggtggt ccacgaagac gagccgcagc ttggcccccc caagtccggg
     2701 caggccctcg gtccgcgcca gcggcaggtc caggtcctgg cgggtgttca cgatctcggc
     2761 cgcggtctct gcggcggcct tggcgtccac cccggcctgg gccaccgggc cgctcaacgg
     2821 gtacagcgcg ccgatcacca cctcgcggcc ctgcgcccag gcccgcggcg cggacgcggc
     2881 cagcgcgccc attcccagga gcttcacgac gtcacgtcta cgcatggcct gcctcctccg
     2941 cccgacggcg gaattcgcct cgtggtgagc ccggccggtg atcactggag accgcttcgc
     3001 gagcgaggat gatcggtcga agaggtcctg gcccgtcacc gttgttcggg cccgcatcgg
     3061 agcataccgg aggcatcgat gcgcccaaga atggcggagc cgccccgcca cgtcaaggcc
     3121 cgcccgcggc gggttgcggg gccgcgcgcg cctggactat cctcgggctc ctactatcct
     3181 caggctcgct cggaggacgc gatggcggag acggtgacct ggcccgacgg cgcgcgctgc
     3241 gccatcatga tgacgttcga cctcgacggc gagagcccgt ggatccaccg cgaccccggg
     3301 ctggccgagc ggccgctcca catgtggatg ggcgcctacg ggcccaagac gggcatgccg
     3361 cgcatcctcg agctcctcga ccgctacggg atcaagacct gcatcttcat cccggggtgg
     3421 atcgtggagc gctaccccgc cctctgcgag gatattctga agcacgggca cgaggtgggc
     3481 caccacggct acctccacga gaagcccttc ttcatgatga gccgcgagga ggaggaagcg
     3541 ctgctcgtga agagcctcga gatcttcaag aaggtgctgg gcgtcgtccc gctcggctcc
     3601 cgcacgcctt ccgccgaccc gagccggcac accatggagc tgctcgcgcg gcacggcttc
     3661 ctctatcaca gcaacgcgct cgacaccgac ctgccctact gccacgagac cgtgcacggc
     3721 cccctcgtgg agttccccac cgcgtggtgc aacaacgacg cgccgttctt cctgtggagc
     3781 gcggtcccgc cggtgggcaa cggaatctgg agccagcagg acgtgtggga gatctggtcc
     3841 gaggagttcg agggcctcta cgcggagggc ggcttcttca actggctcgc ccatccccag
     3901 atcattggcc ggccggcccg tctgcgcatg gtcgaacggc tcatccagct catcctgggc
     3961 aagggccggg tgtggtggcc acggccggtg gacctggccc gcttctggct cgagcgccgc
     4021 aagactccgg tggcctccgc ctgatggccg gcgggcggct cgccggcaag gtcgccctca
     4081 tcaccggcgc gggccgcggc ttcggccgtg ccatcgcgca ggccttcgcc cgcgagggcg
     4141 cgcgggtggc cgcgaactac ctgggcagcc gcgcgggcgc ggaagcggtg gtcgcggagg
     4201 cggagcggct cggcgccgag gcggtcgccc tccgcggcga cgtggcgcgc gaggacgacg
     4261 tgaaggcgct ggtagcggcg actctcgcgc gcttcggacg cctcgacgtc ctcgtcaaca
     4321 acgcgggcat catggtgcgc gccccgatgc tcgcggtccc cgcggaggag tgtcggcgca
     4381 tgttcgacgt caacgtcacc ggcaccatgc tatgcagccg ctacgccctg cccgccatga
     4441 tcgagcggaa gggcggccgc atcatcaacc tctcgtctca gctcgcccag cgcccgctgg
     4501 gcgggggcgg cttcgcggcc tacgcggcca ccaaaggtgc catcgaggcg ctcacccgcg
     4561 ccctcgccgc cgaggtgggc gcgcacggga tcaccgtcaa cgcgatcgcg cccggcggca
     4621 tcgagaccga catgagccgc gacgtgatga ccgaggagta ccggacgcgc cgcctcggcg
     4681 agctgccgct ccgccgcttc ggccgcgtgg aggacgtggc ctattgcgcg gtggtcctgg
     4741 cctccgacga ggccggctac ctcaccgggc agatcctcca cccgagcggc ggctgggtct
     4801 cgggatgacc gcggtgcgct atccgtggcc tcccggccgg aagtccgccg cggtcctctc
     4861 gttcgacttc gacgcggagt ccggcttcct cttccgcgag cccgagaagg cccggcgcag
     4921 cctcggcgac ctggaggagc gccgcttcgg gccccgggtg ggcgtggacc ggatcctccg
     4981 cctgctggat cgcctgcggc tccgcgccac cttcttcatc ccgggctgga cggtcgagca
     5041 ccacttcgag gcgtgccggc ggatccgcga cgcgggccac gagatcggcg cccacggcaa
     5101 cgtgcacgag gcgctcggct tcctcgacga ggagcaggag acccgggtga tgcgcgagca
     5161 gctcgagatc ctggcctcgc gcctgggcgt gcggccggcc ggctaccgct cgccctcctg
     5221 ggacgtcaac gtctggacgc ccgacctgct caagcgccac ggcttcctct acgacagctc
     5281 gctgatgggc aacgacgtgc cgtacgacgt accgacctcc cagggaccgc tcaccgaggt
     5341 gccggtgcag tggctcctcg acgacgcgcc gctcttccgc cacgtctacg gcaccaccaa
     5401 cgcgatcgcc gatccgggcc gcgtgctcca gatgtggagc aaggagttcg cggcgatgca
     5461 cgcggagagc ggctgtttcg tgctgacctg ccaccccttc gtgagcgggc gcgcctcgcg
     5521 catcgccctg ctggacgacc tggtgacgtt catgcgccgc gcgcgcggcg tgtggttcac
     5581 gacctgcgag gagatcgccc gctggcacgc ggccgggatc cgcgatggcg ttggtggccc
     5641 cgtacacgtg acggaagagg ggcgcgtcgt cgagcagcca ctgcaccggc acctcgatca
     5701 gcggtccctc gctggtgtcg atctcgtagg gcacgtcgtt gcccatcagc gagctgtcgt
     5761 agaggaagcc gtgccgcttg agcagcgccg gcgtccacac gttgacgtcc cacgacggcg
     5821 agcggtagcc ggccgggcgc acgccgaggc gcgacttcag gatgtcgagc tgctcgcgca
     5881 tcacccgctc ttcctgcgcc tcgtcgagga agcccagcgc ctcgtgcatg ttgccgtgcg
     5941 cgccgatctc gtggccggcg tcgcggatgc gcgcggattc ggccgggtgg tgctccaccg
     6001 tccatcccgg gatgaagaag gtcgcccgga gcttcagcc
//
LOCUS       DASUFA010000438         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_549373, whole genome shotgun sequence.
ACCESSION   DASUFA010000438 DASUFA010000000
VERSION     DASUFA010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549373"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFX87_15905"
     CDS             complement(<1..215)
                     /locus_tag="VFX87_15905"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4996466.1"
                     /translation="MYIVGIDVGGTFTDLTAVDAETGRVVVTKVPSQPRHEAAAVLAG
                     LGALGIASADVRRLVHGTTVGTNAVLER"
     gene            complement(219..1319)
                     /locus_tag="VFX87_15910"
     CDS             complement(219..1319)
                     /locus_tag="VFX87_15910"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX4996467.1"
                     /translation="MTRPPSAAEAPVEVAPVRPGEQLDWAALEAWLTPRLAELLPAAC
                     GPLEVLQFPNGSANLTYLLRVGPHELVLRRPPFGEIAPGAHDMRREFKVLSRLWSHFD
                     RAPRGYLFCDDRAVLGADFFVMERRRGEVVRDTIPPALRTHPDAGRRLAFALVEAMAD
                     LHALEPAACGLGDLGRPEGFVDRQLDGWAKRWALARPDDAKTDMEEIHARLAARRPAP
                     HRASIVHNDLKLDNCQFAPENPDRVTSIFDWDMTTLGDPLVDLGTLLNYWPDPTDTET
                     TQRGTRPGLALMGLPTRAEITGRYVARTGADPGAVSWWEAFALWKTVVVVVQLHRRWA
                     RGESTDIRMAHIADRAPCLVRAARLVLDRAGL"
     gene            complement(1316..2080)
                     /locus_tag="VFX87_15915"
     CDS             complement(1316..2080)
                     /locus_tag="VFX87_15915"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019663162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4996468.1"
                     /translation="MADPAGLFDLHGKVALVTGGSRGLGRAMVLAFAQAGADVIIASR
                     KLASCEATAAEVREATGRRALPVACHVGHWGQVEALAETAYKEFGRVDVLVNNAGMSP
                     LYDRIENVTEDLWDKVLDVNLKGPFRLTAMVGARMAAGAGGSIIMVSSTSAIRPTANV
                     IPYAAAKAAVNAMTEGFARAFGPTVRVNCIMPGPFLTDISKAWDLEAFRERAKTTMAL
                     GRGGEPEEIVGAALYFASSASSFTTGAVLGIHGGVA"
     gene            complement(2085..2801)
                     /locus_tag="VFX87_15920"
     CDS             complement(2085..2801)
                     /locus_tag="VFX87_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX4996469.1"
                     /translation="MDESAISALTFDVFGTVVDWRTSVTREGEAFGKPRKLSVDWAKF
                     ADAWRGLYQPAMEEVRSGRRPWAKLDELHRESLVRLLGDFGITGVSPAEIDHLNYAWH
                     RLDPWPDAVPGLTRLKRRFTLATLSNGNIGLMVDMAKRAGLPWDAILGAEVARAYKPT
                     ADAYLRSAEALGLRPEQCLMVAAHPADLAAAARCGLRTAYVPRPLEFGPGRPGSQPEP
                     GQPFDVVAGDFVELAEKLGC"
     gene            2932..3249
                     /locus_tag="VFX87_15925"
     CDS             2932..3249
                     /locus_tag="VFX87_15925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX4996470.1"
                     /translation="MLAIWVKVKIKAAMRDKFLKAIEADALGSERDEPGCLRFNVLQD
                     EKDENVYYFYEVYKDQAALEAHRNTPHYAIWRGVTDVLEGPTEPTRCKPVFPAARAYW
                     GKM"
     gene            complement(3253..3807)
                     /locus_tag="VFX87_15930"
     CDS             complement(3253..3807)
                     /locus_tag="VFX87_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996471.1"
                     /translation="MHTTYSLDQFLADTRATIQTKGIPAGLAEIRDHLEKLLHNPELL
                     KKHLGDPVPYVERATIGHDPETDVHVLVHGRPKASKSSVHDHGPCWVVYGNYQNPTRM
                     RRWRRLDDGSRPGHAELTLQREFMNEPGHAAAFAVGDIHSIEFGDDTFFIRVTGGDVE
                     TKETQRFDVEKKTVEIANRAKGQR"
     gene            3867..5081
                     /locus_tag="VFX87_15935"
     CDS             3867..5081
                     /locus_tag="VFX87_15935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996472.1"
                     /translation="MSAPTDRPGFRNDVRVIGLVGAAHFFSHFFQLTLPPLFPLLKDV
                     WGVPYVALGLAMSVFYGASGIGQTVSGFLVDRVGAHRVLLGGMALFSAAIALAGLVPS
                     YWLLLPVALLAGLGNSVFHPADYSILNAAVDPRRIGRGYSVHSISGNLGWAVAPSVVV
                     GLTAHFGWRVALVTVGAAGLAVVSVLATQLRAFVGGRPAERPRTTTGREVRLLLTAPI
                     LSAFAYFAFIATALIGVQTFGVTTLMRIYDTPLGLATGALTAFLVGGATGILAGGLLA
                     DRTRRHDVVAVAGLVTAAGFMMLLATGALAPTLLPIVLGLTGFCHGATGPSRDMLVRA
                     ATPPGASGKVFGFVYSGLDLGSCLTPLAFGWLLDHGDPRMLFVAVGILILCTIGTVVQ
                     VRRSAHPAPAGA"
BASE COUNT          681 a         1775 c         1917 g          794 t
ORIGIN      
        1 cgctcgagca ccgcgttggt gcccacggtg gtgccgtgca cgagccggcg cacgtccgcg
       61 ctggcgatgc cgagcgctcc gagcccggcc agtaccgccg ccgcctcgtg gcggggctgc
      121 gacggcacct tggtgacgac cacgcgcccg gtctcggcgt ccaccgcggt caggtcggtg
      181 aaggtcccgc ccacgtcgat gccgacgatg tacacggctc agagccccgc gcggtcgagc
      241 accaaccggg cggcccggac gaggcacggc gcgcgatcgg cgatgtgggc catgcggatg
      301 tcggtggact cgccgcgcgc ccagcggcgg tgcagctgga ccaccaccac caccgtcttc
      361 cagagcgcga aggcctccca ccagctcacc gcaccgggat cggcgccggt ccgcgcgacg
      421 tagcggccgg tgatctccgc gcgcgtcggc agccccatga gggcgagccc cgggcgcgtc
      481 ccgcgctggg tggtctcggt gtccgtcgga tcgggccagt agttgagtag ggtgcccagg
      541 tccaccagcg ggtcgccgag ggtcgtcatg tcccagtcga agatggaggt cacgcggtcg
      601 ggattctccg gggcgaactg gcagttgtcg agcttgagat cgttgtggac gatggacgcg
      661 cggtggggcg ccggccggcg agcggccagg cgcgcgtgaa tctcctccat gtcggtcttc
      721 gcgtcgtccg gacgggcgag cgcccagcgc ttggcccagc cgtcgagctg gcggtccacg
      781 aagccctcgg gccggccgag gtcgccgagc ccgcacgccg cgggctcgag cgcgtggaga
      841 tcggccatcg cctcgaccag ggcgaacgcc agccggcggc cggcgtcggg atgcgtgcgc
      901 agggccggcg gtatcgtgtc gcggacgacc tcgccgcggc ggcgctccat cacgaagaag
      961 tcggcaccca gcaccgcgcg gtcgtcgcag aagagatacc cgcgcggcgc ccggtcgaaa
     1021 tgtgaccaga gccgcgagag caccttgaac tcgcgccgca tgtcgtgggc gccgggcgcg
     1081 atctcgccga acggagggcg gcgcagcacc agctcgtgcg ggccgacccg gagcaggtag
     1141 gtgagattgg ccgagccgtt ggggaactgg aggacctcca gggggccgca cgcggccggc
     1201 agcagctccg ccaggcgagg ggtgagccac gcttcgagcg cggcccagtc gagctgctcg
     1261 ccgggacgga ccggagcgac ctcgaccggc gcctcggccg cggacggggg acgcgtcagg
     1321 cgacccctcc gtgaatgccg aggaccgcgc cggtggtgaa gctggaggcg ctcgaggcga
     1381 agtagagcgc ggcgccgacg atctcctcgg gctccccgcc gcggccgagc gccatcgtgg
     1441 tcttcgcgcg ctcgcggaag gcttcgaggt cccaggcctt cgagatgtcg gtgaggaagg
     1501 gcccgggcat gatgcagttc acgcgcacgg tggggccgaa ggcgcgcgcg aagccttccg
     1561 tcatcgcgtt caccgcggcc ttggccgccg cgtacgggat gacgttggcg gtgggccgga
     1621 tcgcggaggt gctcgagacc atgatgatcg agccgcccgc gcccgcggcc atccgcgcgc
     1681 cgaccatcgc ggtgagccgg aacgggccct tcaggttcac gtcgagcacc ttgtcccaga
     1741 gatcctcggt cacgttctcg atacggtcgt agagcggtga catgcccgcg ttgttgacca
     1801 gcacgtccac gcggccgaac tccttgtagg cggtctccgc gagggcctcc acctggcccc
     1861 agtggccgac gtggcaggcg acgggcaggg cgcggcgccc ggtggcctcg cggacctccg
     1921 ccgcggtggc ctcgcaggac gcgagcttgc ggctggcgat gatcacgtcg gcgccggcct
     1981 gggcgaaggc cagcaccatg gcgcgcccga ggccgcggct gccgccggtg acgagcgcga
     2041 ccttgccgtg gagatcgaag aggcccgccg gatcggccat gcgatcagca gccgagcttc
     2101 tcggcgagct ccacgaagtc gcccgccacc acgtcgaacg gctgacccgg ctcgggctgc
     2161 gatccggggc ggccggggcc gaactcgagc gggcgcggca cgtaggcggt gcgcaggccg
     2221 cagcgcgcgg ccgcagccag gtcggccggg tgcgcggcca ccatcaggca ctgctcgggc
     2281 cggagcccca gggcctcggc gctgcgcagg taggcgtcgg ccgtgggctt gtaggcgcgc
     2341 gccacctcgg cgcccaggat cgcgtcccac ggcaaccccg cgcgcttggc catgtccacc
     2401 atgagcccga tgttgccgtt cgagagcgtg gccagggtga accgccgctt gaggcgggtg
     2461 aggccgggga ccgcgtcggg ccacggatcg agccggtgcc aggcgtagtt caggtggtcg
     2521 atctcggccg gggagacgcc ggtgatgccg aagtcgccga gcaggcgcac gaggctctcg
     2581 cggtgcagct catcgagctt cgcccacggc cgccgcccgc tgcggacctc ctccatcgcg
     2641 ggctgataga gcccgcgcca ggcatccgcg aacttggccc agtccaccga gagcttgcgc
     2701 ggcttgccga aggcttcgcc ttcccgggtg acgctggtgc gccagtccac cacggtgccg
     2761 aagacgtcga aggtgagtgc gctgatcgcg gattcgtcca tggggtgtct cccgtgaggt
     2821 gcctctcgtc ggggatgcta cgacggtcgg atggtcaagg caagccgcgc ccgtccgggc
     2881 ccgtgtggta atgtcagccg cggctcacca actcgaacag ggagggcgac gatgctcgcg
     2941 atctgggtga aggtgaagat caaggcggcg atgcgggaca agttcctgaa ggccatcgag
     3001 gccgacgcgc tgggctcgga gcgggacgag ccgggctgcc tgcgcttcaa cgtgctccag
     3061 gacgagaagg acgagaacgt ctactacttc tacgaggtct acaaggacca ggcggcgctg
     3121 gaggcgcacc gcaacacgcc gcactacgcg atctggcgcg gggtgaccga cgtcctggaa
     3181 gggcccaccg agcccacgcg ctgcaagccg gttttcccgg ccgcccgcgc ctactggggg
     3241 aagatgtagc ggctagcgct gacccttcgc gcggttggcg atctccaccg ttttcttctc
     3301 gacgtcgaag cgctgggtct ccttggtctc cacgtcgcca ccggtgacgc ggatgaagaa
     3361 ggtgtcgtcg ccgaactcga tggagtggat gtcgcccacc gcgaaggcgg cggcgtggcc
     3421 gggctcgttc atgaactcgc gctggagcgt gagctcggcg tggcccgggc ggctgccgtc
     3481 gtccaggcgg cgccagcggc gcatgcgggt ggggttctgg tagttgccgt agaccaccca
     3541 gcacggcccg tggtcgtgca ccgaggactt gctggccttc ggccgaccgt ggacgaggac
     3601 gtgcacgtcg gtctccggat cgtggccgat ggtggcgcgc tcgacgtagg gcaccggatc
     3661 tcccaggtgc ttcttgagca gctccgggtt gtgcaggagc ttttccaggt gatcgcggat
     3721 ctcggccagc ccggccggga tgcccttggt ctggatggtc gcgcgtgtgt cggcgaggaa
     3781 ctggtcgagg gagtaggtcg tgtgcatggc cgccctcctt gggtgacgac cggatcatag
     3841 cagaacgtcg tgtacaatag ggttccgtgt ccgctcccac cgatcgtccc ggttttcgca
     3901 atgatgttcg cgtcatcggc ctcgtgggcg ccgcccactt cttcagccac ttcttccagc
     3961 tcacgctgcc gccgctcttc ccgctgctga aggacgtctg gggcgtcccg tacgtggcgc
     4021 tcggcctggc catgagcgtg ttctacggcg cctccggcat cggccagacc gtctccggct
     4081 tcctggtgga ccgggttggc gcgcaccggg tgctcctggg cggcatggcg ctgttctcgg
     4141 cggcgatcgc gctggccggg ctcgtgccga gctactggct gctgctgccg gtggcgctcc
     4201 tggccgggct cggcaacagc gtcttccatc ccgccgacta ctcgatcctg aacgcggcgg
     4261 tggacccgcg gcggatcggg cgcggctaca gcgtgcactc gatctcgggc aatctcggct
     4321 gggcagtggc gcccagcgtg gtggtgggcc tcaccgcgca cttcggctgg cgggtggcgc
     4381 tggtgacggt gggtgccgcc gggctggcgg tggtgtccgt gctggcgacg cagctgcgtg
     4441 cgttcgtggg cgggcggccg gcggagcggc cgcggaccac gaccggccgg gaggtgcgcc
     4501 tcctgctgac cgcgccgatc ctgtctgcgt tcgcctactt cgccttcatc gcgaccgcgc
     4561 tcatcggcgt gcagaccttc ggggtcacca cgttgatgcg catctacgac acaccgctgg
     4621 ggctggccac cggcgcgctc accgcgttcc tggtgggcgg ggccaccggc atcctggcgg
     4681 ggggtctgct ggccgaccgg acgcgccggc acgacgtcgt ggcggtggcg gggttggtca
     4741 cggccgcggg gttcatgatg ctgctggcca ccggcgcgct ggcgccgacc ctgctgccga
     4801 tcgtgctcgg cctcaccggc ttctgccacg gggcgaccgg gccctcgcgc gacatgctgg
     4861 tgcgcgcggc cacgccaccc ggcgcgtccg gcaaggtgtt cggcttcgtc tactcggggc
     4921 tcgacctggg ctcctgtctg accccactcg ccttcggttg gctcctcgac cacggcgacc
     4981 cccgcatgct gttcgtcgcg gtcggcattc tgatcctctg caccatcggc accgtggttc
     5041 aggtccgccg cagcgcccac cccgcccccg ccggcgccta gcctggggtc aggtcttaca
     5101 ttccgacatt cctctggtga cgggctgagc cggcgaggcc cagaaatgtc ggaatgtaag
     5161 acctgac
//
LOCUS       DASUFA010000439         4710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_549747, whole genome shotgun sequence.
ACCESSION   DASUFA010000439 DASUFA010000000
VERSION     DASUFA010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549747"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFX87_15940"
     CDS             <1..542
                     /locus_tag="VFX87_15940"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996473.1"
                     /translation="KGAVERVLKTLDDPSLKGEGRLGERRAAVRKIANEIFDFGEIAK
                     RSMARHWQPLSDSQRTEFVGLFADLLERSYISKIETYGGEKIQYTSERADGDFATVST
                     RIVTKNGTEVPVDYRMIKRADRWLVYDVSIEGVSLVSNYRTQFNKIIQTTSYKELVSK
                     LRNKQDELLAEDKGGKKKP"
     gene            539..1693
                     /locus_tag="VFX87_15945"
     CDS             539..1693
                     /locus_tag="VFX87_15945"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4996474.1"
                     /translation="MTGAAFAALLEAQGYDFFTGVPCSMISDLIATLETHPRLPYVPA
                     VREDVAVGLAAGAWLAGRRPMVLMQNSGLGTSLNALVSLSLMYHLPALLLVTWRGHAG
                     KDAPEHLLMGEISPSLLEMIRIPHRVLSAKTIADDIAWGRSESERLSQPVALLLPPGI
                     LGPGSHEAAAPASIAAGTPAPRAALDDPIPAPRISRFEALRAAVAALTSEPVVHANGY
                     ICRESFAVKDRGENFYMIGSMGMASAIGLGVALAAPSRPTVVWDGDGNLLMSLGILPM
                     IGGGPVMGRGRPANLAHVVFDNALYGSTGSQASPSRAVGLHHIARAAGYERATAVAGA
                     DELHAAVATAVAGGGPSFILARVTGEEQPAPRIPYSPEEIRDRFRSCFSR"
     gene            1702..2442
                     /locus_tag="VFX87_15950"
     CDS             1702..2442
                     /locus_tag="VFX87_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX4996475.1"
                     /translation="MKAVILVAGVARRLAPLTDSTHKALLPVGGRSLLDRMLEGLAAC
                     GVEESALVVGHCQDQVRRAAGTSRGTMRISYVENPAYQKGSILSLWCAREIMLSDSTL
                     IMDADVLFPERFLARLIAAPAPSALLLDRGFQDTGEEVKLYAVGDRVIALGKKFVPEA
                     WDVVGEGVGFFKCGAAHAPEYIRLLEESIRETGGANEYEDALHRLLARVPVGWVDVTG
                     LPWTEVDFAEDLHRAETQILPKIESLGR"
     gene            2444..3616
                     /locus_tag="VFX87_15955"
     CDS             2444..3616
                     /locus_tag="VFX87_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4996476.1"
                     /translation="MVRALLYLPDDAARPLVAASVAGRPLAVRAMVAALRAGATLIAV
                     PSTLRDAEVERALRRMPALAAAVRWLTPGAAAPMEDPNPWLLLPASSLIHVTALAGLL
                     AAPAPQGAVLTPSATGPAPVALVPAALVTALWTDLAAGRAVGALLARRLAEGGAETRQ
                     PTGPHVAVREASDLARAEEALQATLGIAVDSGVDRYLHRRCSRWISRLLVQTPVTPNQ
                     VSLSSLAIGLVAVWCFWHATAPSALLGVLLYALASIVDHADGEIARLTFQESRLGANL
                     DWTIDTIIHAGIVLGIGVSSGGWQAIVIGLLAATGVTLSAIFARHLPREIEVGPTVGG
                     VLTHIANRDLFYLVLLSFATLRWLAPSLVFVVAAAVAVGSQIYWVGCLARIRRPRL"
     gene            3616..4587
                     /locus_tag="VFX87_15960"
     CDS             3616..4587
                     /locus_tag="VFX87_15960"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX4996477.1"
                     /translation="MSRWLRPLLVLTGLALVGYLVAQIGPAAIWSSFVTLGWRLPIVL
                     VFPYVLAAAVDTLAWRLLLPTPVPFLLLLRARLAGEAVSLATPTMSVGGDPLKAYLLR
                     PRLPLLDGFVSVIADKTTVVAGQVIFLALGLLAAASLAAPPAGRLLPVMTGLLVVEVL
                     AVGGFALVQASGTLGGGGRLLGRLAKRVGVGGAARLEQGLGDLDRALAALYRQRRRRL
                     VASAVVHAVAWATGGVEIYLVLSLLGTPAPLGIALIVESFASGVKFASFMVPASLGAL
                     EGGYVVFFEAFGMGAAAGLSYVLVRRLREITWAVVGFLALGAQRPRA"
BASE COUNT          562 a         1730 c         1688 g          730 t
ORIGIN      
        1 tcaagggcgc ggtggagcgg gtcctcaaga ccctggacga cccgtcgctg aagggcgagg
       61 gacggctggg cgagcgccgg gcggccgtcc gcaagatcgc gaacgagatc ttcgacttcg
      121 gcgagatcgc caagcggtcc atggcccggc actggcaacc gctctccgac agccagcgga
      181 ccgagttcgt gggcctgttc gccgacctgc tcgagcgctc ctacatctcc aagatcgaga
      241 cgtacggcgg ggagaagatc cagtacacct cggagcgcgc ggacggcgat ttcgccaccg
      301 tgtccacccg catcgtcacc aagaacggga ccgaggtccc ggtggactac cgcatgatca
      361 agcgggccga ccgctggctc gtctacgacg tcagcatcga gggcgtcagc ctcgtgtcga
      421 actaccgcac ccagttcaac aagatcatcc agaccacgtc ctataaggag ctggtgtcga
      481 agctccggaa caagcaggac gagctgctcg ccgaggacaa gggcgggaag aagaagccgt
      541 gaccggtgcc gcgttcgcgg cgctgctgga ggcgcagggc tacgacttct tcaccggcgt
      601 gccctgctcg atgatctcgg acctcatcgc cacgctggag acgcacccgc gcctgcccta
      661 tgtgccggcg gtgcgcgagg acgtggcggt gggcctggcc gcgggggcgt ggctggccgg
      721 gcggcggccg atggtcctca tgcagaactc gggcctcggc acgagcctca acgcgctggt
      781 ctcgctgtcg ctgatgtacc acctccccgc gctgctcctc gtgacctggc gcggccacgc
      841 gggcaaggac gcccccgagc acctgctgat gggcgagatc tcgccgagcc tgctcgagat
      901 gatccgcatc ccgcaccgcg tgctctccgc gaagaccatc gcggacgaca tcgcgtgggg
      961 ccggagcgag tcggagcggc tgagccagcc cgtcgccctg ctgctccccc cgggcatcct
     1021 cgggcccggc tcccacgagg ccgccgcacc ggcatcgatc gccgcgggca cgccggcgcc
     1081 gcgcgcggcc ctggacgacc cgatccccgc gccccgcatc tcgcgcttcg aggcgctgcg
     1141 cgccgccgtc gcggccctga cgtccgagcc ggtggtccac gccaacggct acatctgccg
     1201 cgagtcgttc gcggtcaagg accggggcga gaacttctac atgatcggct cgatgggcat
     1261 ggcctccgcg atcggcctgg gcgtggcgct cgcggcgccg tcgcgaccca ccgtggtctg
     1321 ggacggcgac ggcaacctgc tcatgagcct gggcatcctg ccgatgatcg gcggcggccc
     1381 ggtgatgggg cgcggccgtc cggccaacct ggcgcacgtg gtgttcgaca acgcgctcta
     1441 cggctccacc ggcagccagg cgtcgccctc gcgcgccgtc gggctccacc acatcgcgcg
     1501 ggccgccggc tacgagcgcg ccaccgcggt cgcgggcgcc gacgagctgc acgccgccgt
     1561 ggccaccgcg gtggccggcg gcgggccgag cttcatcctg gcgcgggtga ccggcgagga
     1621 gcagccggcg ccccgcatcc cctactcccc ggaagagatc cgcgatcgct tccggtcctg
     1681 cttcagcagg taggcgattc gatgaaagcc gtcatcctgg tcgccggggt ggcgcggcgg
     1741 ctcgcgccgc tcaccgactc cacccacaag gcgctgctgc cggtgggcgg ccgctcgctg
     1801 ctcgatcgca tgctggaggg gctcgcggcg tgtggagtgg aggagtcggc gctggtggtc
     1861 ggccactgcc aggaccaggt gcgccgcgcc gcgggcacgt cgcgcgggac catgcggatc
     1921 agctacgtcg agaacccggc ctaccagaag ggctcgatcc tgtcgctctg gtgcgcccgc
     1981 gagatcatgc tgagcgattc caccctgatc atggacgccg acgtgctctt ccccgagcgg
     2041 ttcctggccc ggctcatcgc cgcgcccgcg ccgagcgcgc tcctgctcga ccgcggcttc
     2101 caggacaccg gcgaggaggt caagctctac gcggtgggcg atcgggtcat cgcgctcggc
     2161 aagaagttcg ttcccgaagc ctgggacgtg gtcggcgagg gtgtcgggtt cttcaagtgc
     2221 ggtgcggccc acgctccgga gtacatccgc ctcctggagg agtccatccg ggagacgggc
     2281 ggagccaacg agtacgagga cgccctgcac cggctcctcg cgcgggtgcc ggtgggctgg
     2341 gtggacgtca cgggcctgcc gtggaccgag gtggacttcg cggaggacct gcaccgggcc
     2401 gagacgcaga tcctcccaaa aatcgagagt ctcggacgct gatgtggtcc gcgccctcct
     2461 ctatctcccg gacgacgcgg cgcggcctct agtcgccgcc tccgtcgccg gccgtcccct
     2521 cgcggtgcgc gccatggtcg cggcgctccg cgccggcgcc accctgatcg cggtaccctc
     2581 gacgctgcgc gacgcggagg tcgagcgcgc gctgcgccgg atgcccgcgc tggccgccgc
     2641 ggtgcgctgg ctgacgccgg gcgccgccgc acccatggag gacccgaacc cctggctgct
     2701 cctccccgcc tcctcgctga tccacgtcac cgccctcgcc ggcctgctcg ccgcgccggc
     2761 gccccagggc gcggtgctga caccctcggc caccgggccg gccccggtgg cgctcgtccc
     2821 ggcggcgctc gtcactgcgc tctggacgga cctggcggcg gggcgcgcgg tcggggcact
     2881 gctcgcgcgg cgactcgcgg aaggcggcgc cgagacccgg cagccgaccg gaccccatgt
     2941 cgcggtgcgc gaggcgtcgg acctcgcccg cgcggaggaa gcgctccagg ccacgctcgg
     3001 catcgcggtg gactccggcg tggaccgcta tctgcaccgt cgctgctcgc gctggatcag
     3061 ccggctcctg gtgcaaaccc cggtgacgcc gaaccaggtg agcctctcga gcctggcgat
     3121 cggcctcgtg gccgtgtggt gcttctggca cgcgaccgcc ccgagcgcct tgctgggcgt
     3181 cctgctctac gccctcgcca gcatcgtgga ccacgcggac ggcgagatcg cgaggctgac
     3241 gttccaggag tcgcgcctgg gcgccaacct cgactggacg atcgacacga tcatccacgc
     3301 gggcattgtg ctcggcatcg gcgtcagctc ggggggatgg caggcgatcg tgatcgggct
     3361 cctggccgcg accggggtga cgctgagcgc gatcttcgcg cgccacctgc cgcgcgagat
     3421 cgaggtcggg cccacggtcg gcggcgtgct cacgcacatc gccaaccggg atctcttcta
     3481 cctcgtgctg ctctccttcg cgaccctgcg ctggcttgcg ccctcgctcg tcttcgtggt
     3541 ggcggcggcg gtggcggtcg gctcgcagat ctactgggtc ggctgtctcg cccgcatccg
     3601 gaggccgcgc ctctagtgtc ccgctggctc cgtccgctgc tggtgctcac cggcctggcc
     3661 ctggtgggct acctcgtcgc gcagatcggc cctgccgcga tctggtcctc cttcgtcacg
     3721 ctcggctggc ggctgccgat cgtgctcgtc ttcccgtacg tgctggccgc ggcggtggac
     3781 acgctggcgt ggcggctcct cctgcccacg ccggtgccgt ttctcctcct gctccgcgcg
     3841 cggctcgccg gcgaggcggt gagcctggcc acgcccacga tgtcggtggg cggcgatccg
     3901 ctcaaggcgt acctgctgcg gccgcggctc cccctgctcg acggctttgt ctcggtgatc
     3961 gccgacaaga ccacggtggt ggccggccag gtgatcttcc tcgccctcgg cctcctggcc
     4021 gccgcgtctc tcgcggctcc cccggcgggc cggctactgc cggtcatgac cggcctcctg
     4081 gtggtggagg tgctcgcggt cggcggcttc gcgctcgtgc aggccagcgg cacgctgggc
     4141 ggcggcgggc gactgctcgg ccggctcgcc aagcgcgtgg gcgtgggggg cgccgcgcgc
     4201 ctcgagcagg gcctgggcga cctggaccgc gcgctggccg cgctctaccg gcagcgccgg
     4261 cgccgactcg tcgcctccgc ggtcgtccac gcggtcgcgt gggccaccgg cggcgtcgag
     4321 atctacctgg tcctgagcct gctcgggaca ccggcgccgc tcggcatcgc cctgatcgtc
     4381 gaatccttcg cgagcggcgt gaagttcgcc agcttcatgg tgcccgcctc gctcggggcg
     4441 ctcgagggcg gctacgtggt cttcttcgag gccttcggca tgggggcggc cgccggcctc
     4501 tcctacgtgc tggtgcgccg gctacgcgag atcacatggg ccgtcgtggg cttcctggcc
     4561 ctgggcgccc agcgcccgcg ggcctaggtc tgcggccggc gctgggcgcg tgtgctagac
     4621 tctgtgggtc ttgtcgcgtc gccgtatgtt gcggcgacga atcgttgagg aggcggccgt
     4681 gggatctgtt cgcgtcgcgg tggtcggtgt
//
LOCUS       DASUFA010000440         5797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_205310, whole genome shotgun sequence.
ACCESSION   DASUFA010000440 DASUFA010000000
VERSION     DASUFA010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205310"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VFX87_15965"
     CDS             complement(<1..1279)
                     /locus_tag="VFX87_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4996478.1"
                     /translation="MMAEDVVFTPVRQLAEGVRTRRLSPVALAETFLERLERLGPRYN
                     AVVTVTRARALEQARRAEAEIAAGRYRGPLHGIPYGAKDLLATSGGIPTTWGAAPFKD
                     RCFDFDATAILKLEAAGAVLCAKLAMVELAGGMGYRQPNAAFTGPGVNPWNREAWSGG
                     SSSGSGSAVAAGLVPFAIGSETWGSILSPAGNCGLAGLRPTYGRVSRHGAMALCWTLD
                     KLGPLALTADDCGLVLEAIAGRDAADPTTVDRPYRYAVGDPAGRRFRFGVLRGITDGC
                     EDAVRENFTRSLDVLRGLGTVEEIALPELPYDDITRTILFAEAASAFEDLIESGAIAG
                     LTAPEDRYTAYARDAILAKDYLKALRLRGVMARAADRVLAPLDALVAPGRSTVAAMLG
                     GEVRSAIRGTARDVMGAVGNGAGLPAVAVPNGFG"
     gene            complement(1276..1488)
                     /locus_tag="VFX87_15970"
     CDS             complement(1276..1488)
                     /locus_tag="VFX87_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996479.1"
                     /translation="MTDADAVRAEVETLLALVRERYGSRLDAEQSAGVRTAIEGIVQA
                     ARALRAVRLTNADEPAQPFAAYRAEP"
     gene            complement(1589..2209)
                     /locus_tag="VFX87_15975"
     CDS             complement(1589..2209)
                     /locus_tag="VFX87_15975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4996480.1"
                     /translation="MTAAFWPASGEALSFSLNCIITGNNTCSPSVSYGTISFVDNLDG
                     VDVQVNLVGTGQRIQTVLFNYNDSKFANDAFAIAPSVSVAENANTLPPFNGGGFDLQI
                     PSQANMNTPDPYLGTLTLLSVNLDPADFNFKDSSGTLFAAVHIGTCGPNEPATCLPGQ
                     TGTNSIKVGALGDPLTPVPEPATLLLWGTTVAGLGFLKRRRRQSRD"
     gene            2525..3577
                     /locus_tag="VFX87_15980"
     CDS             2525..3577
                     /locus_tag="VFX87_15980"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4996481.1"
                     /translation="MAKRMLFIGAGAIGSYLGAFLARAGHEVTLVDPWPEQVETLRRQ
                     GIAVTGPHDPFQAKPVALHVHELQRLGADFDIGFIAMKAYDTAWATWMALPHLKPEGY
                     LVSAQNCWNDPILASIAGAERSVGLIMSKIGVALWKPGQVERGMEKGQGAGHDVFRAG
                     EHDGRITERVTELADIMSVVDGARPTDNLWGERWSKLCANCMGNPVQAMTGLGSIEIA
                     GQARGREITIRIAQESAAVGLKLGYRIPKFNGRETEQWAQAGRPDVYRELDAALTPKE
                     GSGRNWRASMAQDVIKGRRSEIDYMNGLVVNKGREVGVPTPVSSAVVGLMREVDAGTR
                     KPSPEHIELALKRAGS"
     gene            complement(3599..4255)
                     /locus_tag="VFX87_15985"
     CDS             complement(3599..4255)
                     /locus_tag="VFX87_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996482.1"
                     /translation="MSRLVLTLALALLAAGCAPATEPRTVIAAGPRVLFDDELRTARN
                     WAAGAGTICRSTYAADGFVVENIAPSAPCLLGPVHPESFPAGVRIEITARLRKGTREG
                     AFGLMFASRGGADNRTFATFGLTANGTYRVASWSGGKWNYPVPPTATRSVKTEYGAPN
                     TLAVELRARSIVAYVNGRPVATAELSTEATGTLGLYVDQRGMEVLFNNLRVSELPPIR
                     "
     gene            4353..5402
                     /locus_tag="VFX87_15990"
     CDS             4353..5402
                     /locus_tag="VFX87_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4996483.1"
                     /translation="MRGVVFTGNRQLELRDFPDPTPGPGEVVLEIKASGMCGSDLKFY
                     RADGNETASLGLGGSGKKVIAGHEPCGVIAAVGPGVSDAEGRVGQRVMNHHYTGCGRC
                     KHCRVGWSQLCKAGITVFGATGDGAHAKYMKVPARTNVPLPDELTFEEGAAVSCGTGT
                     AYGALKRIDVSGRDTLAVFGQGPVGLSATMLGVAMGARVIAVDIAAERLALAKQFGAG
                     AVINSRETDPVQAIMDLTHGEGAEAAMDCTGLPEPRVAAVRSTATWGRMCFVGEGGAT
                     SLDVSRDLIRKQLTLVASWTFSTMGQWECARFVADRKIPLGRIFTDRFGLDQAVEAYK
                     RFDTQTTGKGVFTSF"
     gene            5420..5605
                     /locus_tag="VFX87_15995"
     CDS             5420..5605
                     /locus_tag="VFX87_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996484.1"
                     /translation="MRAQYHLRQCFVFLNMCAVNQPEVLIANAAQRFNEKGELTDETS
                     RQLIRKLVAWTRRLGES"
BASE COUNT          924 a         2000 c         2072 g          801 t
ORIGIN      
        1 ccccgaaccc gttcggcacc gccaccgcgg gcagacccgc gccgttgccg accgcgccca
       61 tcacgtcgcg cgcggtcccg cggatcgcgc tgcgcacctc gccccccagc atcgcggcca
      121 ccgtggaacg gccgggcgcc accagcgcgt ccagcggagc cagcacgcgg tccgccgcgc
      181 gcgccatcac gccccgcagc cggagcgcct tcagatagtc cttggcgagg atcgcgtcgc
      241 gcgcgtaggc ggtgtagcgg tcctccggcg cggtgaggcc cgcgatggcg ccgctctcga
      301 tcaggtcctc gaacgcgctc gccgcctccg cgaagaggat cgtgcgcgtg atgtcgtcgt
      361 agggcagctc cggcagcgcg atctcctcga cggtgccgag cccgcgcagc acgtccagcg
      421 agcgcgtgaa gttctcccgc accgcgtcct cgcagccgtc ggtgatgccg cgcagcacgc
      481 cgaagcggaa gcgccgcccc gccggatcgc ccacggcgta gcgatagggc cggtccaccg
      541 tggtcggatc ggcggcgtcg cgccccgcga tggcctcgag caccaggccg cagtcgtccg
      601 cggtgagggc cagcgggccc agcttgtcga gcgtccagca gagggccatg gcgccgtgcc
      661 ggctcacccg cccgtaggtc ggccggagcc ccgcgagccc gcagttgccg gcgggcgaga
      721 ggatggagcc ccacgtctca gacccgatgg cgaagggcac gagcccggcc gcgaccgcgg
      781 agcccgagcc gctcgacgac cccccgctcc acgcctcccg gttccacgga ttcacgccgg
      841 gtccggtgaa cgcggcattg ggctggcggt agcccatgcc gccggccagc tccaccatgg
      901 cgagcttcgc gcagagtacc gcgcccgcgg cctcgagctt gagaatcgcc gtcgcgtcga
      961 agtcgaagca gcggtccttg aagggcgcgg cgccccacgt ggtcggaatg ccgccggagg
     1021 tcgccagcag gtccttggcg ccgtacggga tgccgtggag cgggccccgg tatcgcccgg
     1081 ccgcgatctc cgcctcggcg cgccgcgcct gctcgagcgc gcgggcgcgc gtgaccgtga
     1141 ccaccgcgtt gtagcgcggg ccgagacgct ccagccgctc gaggaacgtc tcggccagcg
     1201 cgaccgggga gagacggcgg gtgcggacgc cctccgcgag ctgccgcacc ggggtgaaga
     1261 ccacgtcctc ggccatcatg gctccgcgcg gtaggccgca aagggctggg cgggctcgtc
     1321 ggcgttggtg aggcgcaccg cgcgaagggc gcgcgcggcc tgcacgatcc cctcgatcgc
     1381 ggtgcgcacg ccggccgact gctcggcgtc caggcggctg ccgtagcgct cgcggaccaa
     1441 ggcgagcagc gtctccacct ccgcgcgaac cgcgtcggcg tcggtcatgc cgcccgcatt
     1501 ctagcggagg cgcggatcga gcgcatcgcg gagaccggcg tgtcggggtg cctccgagac
     1561 gtggtacggg cgaaccgtta cagccggtct agtcgcgcga ctggcggcgc cgacgcttca
     1621 ggaacccgag gccagctacc gtggtgcccc agagcagcag cgtggccggc tcgggaaccg
     1681 gggtcagcgg atcgccgagg gctccgacct tgatcgagtt ggttccggtc tgaccgggta
     1741 gacaggtcgc cggctcattg gggccgcaag tcccgatgtg cacggccgcg aaaagggtgc
     1801 cggacgagtc cttgaagttg aagtccgcag gatcgagatt gacgctcagg agcgtgagcg
     1861 tgccgagata cgggtcgggc gtgttcatgt tggcctggct cgggatctgc aggtcgaacc
     1921 ccccgccatt gaacggtggc agggtattcg cattctccgc gacagagacg gagggcgcga
     1981 tggcgaacgc gtcattggcg aacttgctgt cgttgtagtt gaacaagacg gtctggatcc
     2041 gctggccggt acccacaagg ttgacctgga cgtcgacgcc gtccaaattg tcgacgaagg
     2101 agatcgttcc gtaggagacc gaaggcgagc acgtgttgtt gccggtgatg atgcagttga
     2161 ggctgaaaga cagtgcttcg cctgaagcgg gccagaacgc ggccgtcatc agaccaacga
     2221 gggccgcaac cttcaaccct atacgagtca ttcctttgtc ctccttggct cgaaacctcg
     2281 tgcgcccggc taggaaagat ttggagaaca gcaagttatg ggccagggta gaaggtgact
     2341 cgtaaaagac tgccattatt gccttcctga caaatcgggc aagccgatta tggaaacgtc
     2401 ttgcccaata ctggccaagc ttctgcacag gtaaggaaag cagaagacac cgtttatcgc
     2461 cgcccgggtt ggcgctaaga tcacgggggc cctcccgagg agggccggcg aaagggaggc
     2521 ccagatggcc aagcgaatgc tcttcatcgg cgcgggtgcg atcggcagtt acctgggcgc
     2581 gttcctcgcg cgggccggcc atgaggtcac cctggtggat ccgtggcccg aacaggtcga
     2641 gacgctgcgc cgccagggca tcgcggtgac cggcccgcac gaccccttcc aggccaagcc
     2701 cgtcgcgctg cacgtgcacg aactgcagcg actgggcgcc gacttcgaca tcggcttcat
     2761 cgccatgaag gcctacgaca ccgcgtgggc cacctggatg gcgctgcccc atctcaagcc
     2821 cgagggctat ctggtctccg cccagaattg ctggaacgat cccatcctgg cgagcatcgc
     2881 gggcgcggag cgctcggtcg ggctcatcat gtcgaagatc ggcgtcgccc tgtggaagcc
     2941 gggccaagtg gagcgcggca tggagaaggg ccagggtgcc gggcacgacg tgttccgggc
     3001 gggcgagcac gacgggcgca ttaccgagcg ggtcaccgag ctggccgaca tcatgagcgt
     3061 ggtggacggc gcgcggccca ccgacaacct gtggggcgag cgctggtcca agctctgcgc
     3121 caactgcatg ggcaacccgg tgcaggccat gaccggcctc ggctccatcg agatcgccgg
     3181 gcaggcgcgg gggcgcgaga tcaccattcg catcgcgcag gaatccgccg cggtggggct
     3241 caagctcggc taccgcatcc ccaagttcaa cggccgtgag acggagcagt gggcgcaggc
     3301 cggccgcccc gacgtctacc gggagctcga cgccgcgctc acccccaagg aaggctccgg
     3361 ccgcaactgg cgcgcctcca tggcccagga cgtgatcaag gggcgccgca gcgagatcga
     3421 ctacatgaac gggctcgtgg tgaacaaggg ccgcgaggtc ggcgtgccca ctccggtatc
     3481 gagcgcggtg gtggggctga tgcgcgaggt ggacgcgggg acgcggaaac cctcgcccga
     3541 gcacatcgag ctcgcgctca agcgcgcggg aagctgaccc cccgcgggat caactggatc
     3601 agcggatcgg cggcagctcg ctgacccgga ggttgttgaa gagcacctcc atgccccgct
     3661 ggtccacgta gagccccagc gtgccggtgg cctccgtgga cagctccgcg gtcgccaccg
     3721 gccggccgtt gacgtacgcc acgatggacc gggcgcgcag ctcgacggcc agggtattcg
     3781 gggccccgta ctcggtcttc acgctccggg tcgcggtggg cggcaccggg tagttccact
     3841 tcccaccgct ccagctcgcc acccggtagg tcccgttcgc ggtgagtccg aacgtggcga
     3901 aggtgcggtt gtcggccccg ccgcggctgg cgaacatgag cccgaacgcg ccctcccgcg
     3961 tgcccttgcg cagtcgggcc gtgatctcga tgcgcacgcc ggccgggaac gactccgggt
     4021 gcaccggtcc gagcaggcag ggggcggagg gcgcgatgtt ctcgacgacg aagccgtcgg
     4081 cggcgtacgt cgaccggcag atggtgccgg cgccggccgc ccagttacga gcggtgcgga
     4141 gctcgtcgtc gaagagcacg cggggaccgg cggcgatcac ggtgcgcggc tcggtcgcgg
     4201 gggcgcagcc ggcggcgagg agcgccagcg ccagcgtcag gacgagccga gacatcgatc
     4261 gcctccagga tcgtgagggt gagcgcttct ggccgtcggc cacctcatct tttacactac
     4321 actgggttca aacgacaacg agggggtggc ccatgcgcgg agtggtattt accggcaacc
     4381 ggcagctcga gctccgagac ttccccgatc cgacgccggg cccgggcgag gtggtgctgg
     4441 agatcaaggc ctccggcatg tgcggcagcg atctcaagtt ctaccgcgcg gacggcaacg
     4501 agaccgcctc cctcggcctg ggcggcagcg gcaagaaggt gatcgcgggg cacgagccct
     4561 gcggggtcat cgcggcggtc ggccccggcg tgagcgacgc cgaggggcgc gtgggccagc
     4621 gcgtgatgaa tcaccactac accggctgcg gccgctgcaa gcactgccgc gtcgggtggt
     4681 cgcagctctg caaggccggg atcaccgtct tcggcgccac cggcgacggc gcccacgcga
     4741 agtacatgaa ggtgcccgcc cgcaccaacg tgcccctgcc cgacgagctc accttcgagg
     4801 aaggcgccgc ggtgtcctgc ggcaccggca ccgcctacgg cgcgctcaag cgcatcgacg
     4861 tgtccggccg cgacaccctg gccgtcttcg gccagggccc ggtcggcctc tccgccacca
     4921 tgctgggcgt ggcgatgggc gcgcgcgtca tcgcggtgga catcgcggcc gagcggctgg
     4981 ccctcgcgaa gcagttcggc gccggcgcgg tcatcaactc gcgcgagacc gacccggtgc
     5041 aggccatcat ggacctgacg cacggcgagg gcgcggaggc cgccatggac tgcaccggcc
     5101 tgcccgagcc tcgggtggcc gcggtgcgga gcacggccac gtgggggcgc atgtgcttcg
     5161 tgggcgaggg gggcgccacc agcctcgacg tgagccgcga cctgatccgc aagcagctca
     5221 ccctggtggc ctcgtggacg ttcagcacca tggggcagtg ggagtgcgcc cgcttcgtgg
     5281 ccgaccgcaa gatccccctc ggccgcatct tcaccgaccg cttcggtctc gaccaggccg
     5341 tggaggccta caagcgcttc gacacgcaaa ccaccggcaa gggcgtgttc acctcgttct
     5401 aggcgggagg agaccgatcg tgcgcgccca gtaccacctg cggcagtgct tcgtcttcct
     5461 caacatgtgc gcggtcaacc agccggaggt gctgatcgcg aacgcggccc agcgcttcaa
     5521 cgagaagggc gagctgaccg acgagacctc aagacagttg atccggaagc tggtggcctg
     5581 gacgcggagg ctgggtgaga gctagaccac ccctcaccgt agaccacccc tcaccctccc
     5641 ctctcccctc aggggagagg gggaattcga accctcgccc ccggaggggg agagggcagg
     5701 gtgagggggt caggccgcga cccgctgcca gccaccgtcc ttgagcgagc gcagcaccgc
     5761 gtccagcacg agctgggcgg cgaccccgtc ctcgagc
//
LOCUS       DASUFA010000441        16947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_205668, whole genome shotgun sequence.
ACCESSION   DASUFA010000441 DASUFA010000000
VERSION     DASUFA010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 16947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16947
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205668"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1804
                     /gene="pgm"
                     /locus_tag="VFX87_16000"
     CDS             164..1804
                     /gene="pgm"
                     /locus_tag="VFX87_16000"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX4996485.1"
                     /translation="MSLSPLAGKPAPPGMRVDVRALERDYYARRPDTGDPDQLVAFGT
                     SGHRGSPLRGSFTEAHILATTQAICDYRRGRGIDGPLYMGKDTHAVSGPAERTALEVL
                     AANGVETIIQREDGVTPTPVISHAILVYNRGRLTHPADGIVVTPSHNPPEDGGFKYNP
                     PDGGPADTDVTRWVQDRANELLRAGNAGVARVPYERALRAATTHPEDLVLPYVNDLRN
                     VVDMDVIRGAGLRLAVDPLGGASRPYWEPINAVYGLDVAIVNPTIDPTFSFMTVDHDG
                     KIRMDCSSPYAMARLVGLKDRYRVAFANDPDSDRHGIVTPGAGLMNPNHYLAVAIGYL
                     FTSRPEWPAGAAIGKTLVSSGLIDRVARKLGRRLYEVPVGFKWFVPGLLDGSLGFGGE
                     ESAGASFLRRDGTAWTTDKDGLIMDLLAAEITARTGRDPGQHYEALAAELGTPCYTRL
                     DAPATSEQRTRLGKLSPTAVTASQLAGEPITATLTRAPGNDAPIGGLKVVAASGWFAA
                     RPSGTENIYKIYAESFRDAAHLDAIVREAQEIVDAALR"
     gene            complement(1801..2979)
                     /locus_tag="VFX87_16005"
     CDS             complement(1801..2979)
                     /locus_tag="VFX87_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX4996486.1"
                     /translation="MKITKVESLHADGGWRSLDFLKISTDEGIVGWSEYNESFGGVGV
                     SAAIAGLAPMLIGRDPRPWEAHVALLSAVRRQATGGVIQQAIGAIENALLDIKARSLD
                     VPVYALFGGPLRDRIRLYWSHCATYRVSRAREMQIPPVRTLDDIVAVGREVVAKGYTA
                     LKTNVLLLGDNPRAHVPGFGRSAGFPELNADRHVLDALRAELAAFREGAGPGTDILVD
                     LNFNYKTEGFLKMAWAMEPYDLLWVEMDTRDPKALAYVRSRTTIPVASCECLFGRRDY
                     RPFFEQQAVDVAIIDTPWNGVGESLKIAAMADTYEINVAPHNFYGHLATMMNAHWSAV
                     VPNLRIMETDPDMVPWQDDLVTVKPRIENGYLLLPTGPGWGTEVNEEAVRAHPPKTGV
                     "
     gene            3061..3726
                     /locus_tag="VFX87_16010"
     CDS             3061..3726
                     /locus_tag="VFX87_16010"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HEX4996487.1"
                     /translation="MARRSGRAVFLLMVGLVGAACLGPVRGLYPPQPGDATVSVHVVR
                     HGWHSGLVIRRDQIPMGAWPELGRFPATRFLEVGWGDRAFYQSPDAGVALAIEASFAS
                     EGSVLHVTGLDRPPAEHFAHAEITAVELSARGAESLARFVGRAYARDPAGAPIDLGPG
                     FYPGSRFYAATGRYSLVYTCNSWIADALRAGGCPITPAWALTGGNLAFQARRCGPPAH
                     RYP"
     gene            3802..5415
                     /locus_tag="VFX87_16015"
     CDS             3802..5415
                     /locus_tag="VFX87_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4996488.1"
                     /translation="MSGSLATVPGREVCVARYLVQRNAAEHGDRPFLVFPDAPTLTWR
                     GAHDRVVQIACGLARLGVRQGEHVLSWLPTGTDSILLMLALNHLGAVYVPVNLAYRGR
                     LLEHVVRLSDARLMVAHADLVPRLEEIDLARLEAVVVLGGAAATGRLAGHGPEVLAPS
                     NRELPPLPRPIEPWDTQTVIFTSGTTGPSKAVLSSYCQGWSMMGPEAWPFITGADRYF
                     MTMPMFHVGGTGLVNCMTFRAGSLAVVPSFDTESFWPLVRRTEATVVFLLGAMASFLW
                     KRPPGPGDRDHKLKLAFMVPLVDDVPGFAARFGIDVRTVFNMTEINNPIVSEPQPQGR
                     GFCGRLRPGSEARLVDANDCAVPPGEVGEMILRTARPWALNHGYHNDPAATARAWRNG
                     WFHTGDAFRQDALGNYYYVDRVKDAIRRRGENISSYEVEIELLSHPAVREAAAVAVPS
                     EHGEDEVMAVLSLVPGASLDPAELVAFLRPRLAHFMIPRYVRITAELPKTPTQKAEKH
                     RLRAEGVTPDTWDREVAGIRVRREKVGGV"
     gene            complement(5428..6846)
                     /gene="ahcY"
                     /locus_tag="VFX87_16020"
     CDS             complement(5428..6846)
                     /gene="ahcY"
                     /locus_tag="VFX87_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX4996489.1"
                     /translation="MSATTKSTRPQDHVVTDLSLADWGRKEIQIAETEMPGLMAIREE
                     YASQQPLKGARITGSLHMTIQTAVLIETLQALGADVRWASCNIFSTQDHAAAAIAAEG
                     TAVFARKGESLEEYWDYTHRIFEWPDGAYSNMILDDGGDATLLLHLGAAAEKNASVIA
                     HPANEEETALFAAIKAKLGKDPNWYSTRLPKIRGVSEETTTGVKRLYQMAKEGRLKFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKVAVVCGYGDVGKGSSQALRA
                     LSAQVWVTEVDPICALQAAMEGYRVVTMEEAASQADIFVTATGNYHVITHEHMKRMKN
                     NAIVCNIGHFDSEIDIASLKQYKWENIKPQVDHVIFPDGKRIILLAEGRLVNLGCGTG
                     HPSYVMSSSFANQVIAQIELFTNPEKYPIGVYVLPKHLDEKVARLQLKKLNASLTELT
                     DAQARYIGVEKTGPYKPDHYRY"
     regulatory      complement(6871..6962)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            7004..7525
                     /locus_tag="VFX87_16025"
     CDS             7004..7525
                     /locus_tag="VFX87_16025"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4996490.1"
                     /translation="MLTAADFIRAQLKRLHTGLDQALAELTPEQLHTVPAGHPKANTI
                     GWGIWHYVRTEDNIVRWVIQDRRPPIWTESGYGQKLGLPPAAQGTGMSTEEAQALRIN
                     DIPLFQEYTRKVWAGTEELFDAATPALLEKMVMVKPLGEMSVVQCLGQVGLTHGMTHL
                     GEIGLARTLIAGS"
     gene            7533..8603
                     /locus_tag="VFX87_16030"
     CDS             7533..8603
                     /locus_tag="VFX87_16030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996491.1"
                     /translation="MRFGWLTLSHSPSAADDHTAIEEQLTQACLAEEVGFDSVWLTEH
                     NFTGEAVYCDPIPFASALAARTTRVRIGFAVIQLALRHPVRLAVQLALLDNLSRGRLD
                     VGVGRGTLYNEYEYVGYGLRSDDSRERSAEALEILTRAWTEEPFSYQGKYFQVSLPAL
                     RPRVYQRPHPPIWRSVVTPGSFAECGRQGVPILTSRLPLGVLGERLKLYEDGLIAGGH
                     PAPVRDRLRRDVAVWRHVYVGESHAEAEDRLADTLAHTRRHMLGARAAHNPEDYRVDP
                     AVLNAFNDPGVSEDEAVRQSMATGALYGTARQVAERLAELRAAGVRHVLCQMSFGYLG
                     HDRIVDSMRRFGLQVMPAFRDY"
     gene            complement(8600..10555)
                     /locus_tag="VFX87_16035"
     CDS             complement(8600..10555)
                     /locus_tag="VFX87_16035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4996492.1"
                     /translation="MPTPRAMVPQDLTRIRFVSDPQIAPDGRTVAFVVTTLSEEQDEY
                     LSNIWVVNATGGEPRRFTTGPRRDTSPRWSPDGSHLAFISEREPGKKGQLHVMPAAGG
                     EPIRLTDLRHGLSAPVWSPDGTRLAFAARVGGPPEPVSEEDKRRSKPPRVITTMNYRW
                     NGEGFTYDRRPQIFVVAADGGEPRQLTEGDHDHAEPAWAPDGRSIVFTSARHDDRDHD
                     DATDIWLVAAEGGPPRRLTPTAGPAGHAAFSPAGDTIAYLGRSEANAFGNNIRLFAMP
                     ATGGASRCLTAAFDRSCSPLGVPPLWSPDGSAITVAAEDHGSLGVYRVPAAGGPATQI
                     VGGERVVAGFSASRDGKTVAFAASGPDAPAEVFVCDADGGAEKQLTTCNRDWKGEVAL
                     SRPERFRFERAGMTVDAWIMKPFGFEAGKRYPLLLNVHGGPHAQYGYPFLDEFQVQAG
                     AGYAVLYTNPRGSQGYGEAFTRAVVGDWGGGDAADVQAGLDEALRRYDWIDPGRLGLL
                     GGSYGGFMTSWIVGHSTRFKAACSERAVNSHVTMFGSSDIGHLFNVGELGGIFPWENM
                     GKYLERSPLTYARDITTPLLIIHSEDDLRCPIGEAEQLFMALKTLRRVAVLVRFPDEN
                     HELSRSGKPRHRLERFRIILDWFAKYV"
     gene            10683..11873
                     /locus_tag="VFX87_16040"
     CDS             10683..11873
                     /locus_tag="VFX87_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX4996493.1"
                     /translation="MRPFISISLAVLLALALASPAPAQAPPPWKQGQPAEMANSPLAP
                     IAQPPAPKPPGEIPVDKIKVPRGFKVSLWAHGLNNARAMTWGDKGTLFVSSRVAGNVY
                     AVVDRGGQREVKTIAKGLNLPNGVAFKDGTLYIAEVSRITKMAGIEDKLDSPPAMEVV
                     YDILPRDLPHGWKYLAFGPDGKLYFNIGAPCNICIPPDTHANISRVNPDGTGFEYVAH
                     GVRNSVGFDWHPVTKELYFATHARDWLGEDVPSDRFDVASKKGLHFGFPYCHQGDILD
                     PDFGRGRSCSEFAPPLLKTGPHVAGNGVEFYTGSMFPAEYKNRVFLAQRGSWNRTQKS
                     GFKVMMVTLRTGDVPRYEVFAEGWLQGDQVWGRPVYTRQMKDGSLLIADDYAGAIYRV
                     TYQP"
     gene            11885..12469
                     /locus_tag="VFX87_16045"
     CDS             11885..12469
                     /locus_tag="VFX87_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEX4996494.1"
                     /translation="MLAGLSLAAVVAAAGGAPALAADLEAGRRKAEPCAACHGRDGHA
                     TVPGTPSLAGQPVYFTHWQLIKYRDGRRKDPVMAPLAATLSDADMADLAAYYAAQRPT
                     RRPAITDPARVAAGRPLADLYHCSSCHRPGFVGQEQAARLAGQDLDYLLRLLRAFKSR
                     TASDLDGTMTVATQPLTDDEIVSLVHYIATLGEP"
     gene            12737..13234
                     /locus_tag="VFX87_16050"
     CDS             12737..13234
                     /locus_tag="VFX87_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996495.1"
                     /translation="MHIVMVTLIALVAAAPAVAQAQVQVHVDIGLRLPAPPRLVVVPQ
                     VPAVRYVAEPAAPGNLFFYSGQYWAFASGGWYVSGGYNGPWVVVAPEFVPRPVLFVPV
                     QYYHVPPGHWKKWAPQRPPRWQEDWGREWADKREWKSRDRDRDDDDRDDRRGKSKGKG
                     KGHGR"
     gene            complement(13250..13972)
                     /locus_tag="VFX87_16055"
     CDS             complement(13250..13972)
                     /locus_tag="VFX87_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996496.1"
                     /translation="MLALARRFDFERDTFAFPNELVWEYHFDATTGQTRVVRREPAPR
                     YALRCFVLVRAARQFFYHARFEPTIEPADDLTYRRLIRAVLARPPRRPSPPGERVVIP
                     GYTGLRELSAAREPVVKAACGGAWRSYVQHSHWRIVVPVSRGHQHRTAGQLLAAVEDH
                     GAPLVHLLRFPSLAINHGMVVFEGARTGAGAEFLAYDPNDPERPARLTYDRKRRAFSL
                     SANRYWRGGELNVFEIFRRWYL"
     gene            14075..14431
                     /locus_tag="VFX87_16060"
                     /pseudo
     CDS             14075..14431
                     /locus_tag="VFX87_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
     gene            14703..14843
                     /locus_tag="VFX87_16065"
                     /pseudo
     CDS             14703..14843
                     /locus_tag="VFX87_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017543351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
     gene            14888..15589
                     /locus_tag="VFX87_16070"
     CDS             14888..15589
                     /locus_tag="VFX87_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996497.1"
                     /translation="MADQTDDIAGRIVLIVDASVPAATRAACDDAAATLARCAGMPVT
                     LAVVPIATVDAVQPVIRRGPGQIRDLEPPSVAGAGDAMTSAPFVWRRDGRPDWGAMWT
                     TFCDLALHGGPPHRGALQALRGPSIGDRDAVSAPEISAELRRGIEETTGLATAPAEPG
                     WIAITCASARMAAWLCATIILENVDARVEGTRLLVPAGPGFALEDQVKSVITVVAKTH
                     HYWREHHESAREPRS"
     gene            15625..16020
                     /locus_tag="VFX87_16075"
     CDS             15625..16020
                     /locus_tag="VFX87_16075"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX4996498.1"
                     /translation="MAGLEADVVELTAQERRRARRRVVTRAGRVFTLALPAGDVLVPG
                     RVLHVGPGWYVTVEAAPEALLAITPRTPREGLRVALEVGRLHAALAVAGGRLLVPDEP
                     AVERLLRRLGVPWVRAREPFLPVSSGSPH"
     gene            16144..>16947
                     /locus_tag="VFX87_16080"
     CDS             16144..>16947
                     /locus_tag="VFX87_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX4996499.1"
                     /translation="MTRERTIRGQVAVVGVGETTYYKHGRAPESEFALALRAILRACA
                     DAGINPREIDGFASYSNDRNDPSRLAAALGLPELRFSNMQWGGGGGGGSAAVGNAAAA
                     VAAGYADCVVVFRALAQGQFQRFGAAPPGGTVSGEAALTFPYGLISPAQRFAMRVMRF
                     MHDHGVRPEAQRAIALASYHHAQANPRAVMHGRPLTPEAYDASRFIVEPFRLFDCCME
                     NDGAAALVLVAAERARDLRRPPAYLLGVAQGSEYRNAARGHNAPTYASSS"
BASE COUNT         2378 a         6016 c         6051 g         2502 t
ORIGIN      
        1 tcaaggatct ggagagccac gccgactacc cttcggacgt cgccgactgg tacgcgatga
       61 tggcccgcga ggtcatgcga gcgctcgagc aaaccgacgc gcatcagcgg gtcgcctcgg
      121 acgacgcgct ctggagcggc ccgcggcccg cctcgggtag cccgtgagcc tttctccgct
      181 cgcggggaag ccggccccgc ccgggatgcg ggtagacgtc agggcgctcg agcgcgacta
      241 ctacgcacgc cggcccgaca ccggcgatcc agaccagctc gtcgccttcg gcaccagcgg
      301 gcaccgcggc tccccgctgc gcggctcctt caccgaggcg cacatcctgg cgaccaccca
      361 ggcgatctgc gactaccggc gcggccgtgg catcgacggc ccgctgtaca tgggcaagga
      421 tacccacgcg gtgtccgggc cggccgagcg caccgcgctc gaggtgctgg ccgccaacgg
      481 cgtggagacg atcatccagc gggaggacgg cgtgacgccg acgcccgtca tctcgcacgc
      541 gatcctggtg tacaaccgcg gtcgccttac gcacccggcg gacggcatcg tcgtgacccc
      601 gtcgcacaac ccgccggagg acggcggctt caagtacaac ccgcccgacg gcggccccgc
      661 cgacaccgac gtgacccgct gggtccagga ccgcgccaac gagctcctgc gggcgggcaa
      721 cgcgggcgtc gcgcgcgtgc cgtacgagcg cgccctccgc gccgcgacca cgcatccgga
      781 agacctcgtc ctgccttacg tgaacgatct gcggaacgtc gtggacatgg acgtcatccg
      841 cggcgccggg ctccggctcg cggtggaccc gctgggcggc gcgtcccggc cctactggga
      901 gccgatcaac gcggtctacg gactcgacgt ggcgatcgtc aacccgacga tcgacccgac
      961 cttctcgttc atgacggtgg atcacgacgg caagatccgc atggactgct cgagtccgta
     1021 cgcgatggcc cggctcgtcg gcctcaagga tcgctatcgc gtcgccttcg cgaacgaccc
     1081 ggattcggat cgtcacggta tcgtcacgcc cggagcgggt ctgatgaatc ccaaccacta
     1141 cctggccgtc gccatcggct acctgttcac gagccggccg gagtggccgg cgggcgccgc
     1201 gatcggcaag acgctggtca gcagcgggct gatcgaccgg gtggcccgga agctgggccg
     1261 gcgcctgtac gaggtgccgg tggggttcaa gtggttcgtt cccggtctgc tcgacggctc
     1321 gctcggcttc ggcggcgagg agagcgccgg cgccagcttc ctccggcgcg acggcaccgc
     1381 gtggacgacc gacaaggacg gcctgatcat ggacctgctc gccgccgaga tcaccgcgcg
     1441 caccggtcga gaccccggcc agcactacga ggcgctggcc gcggagctcg gcacgccctg
     1501 ctacacccgc ctcgacgccc ccgcgacgtc cgagcagagg acccggctcg gaaagctgtc
     1561 gccgacggcg gtcacggcct cgcagctcgc aggcgagccc atcaccgcca cgctcacccg
     1621 cgcccccggc aacgacgcgc cgatcggcgg cctcaaggtc gtggccgcga gcggctggtt
     1681 cgcggcgcgg ccctccggga cggagaacat ctacaagatc tacgcggaga gcttccggga
     1741 cgccgcgcac ctcgacgcga tcgtgcgcga ggcgcaggag atcgtggacg ccgcgctgcg
     1801 ctagacgccg gtcttcggcg ggtgcgcgcg gaccgcctcc tcgttcacct cggtgcccca
     1861 gccggggccg gtgggcagca ggaggtagcc gttctcgatc cgcggcttca cggtcaccag
     1921 gtcgtcctgc cacggcacca tgtcgggatc ggtctccatg atgcgcaggt tggggaccac
     1981 cgcgctccag tgcgcgttca tcatggtggc caggtggccg tagaagttgt gcggggccac
     2041 gttgatctcg taggtgtcgg ccatggccgc gatcttgagc gactcaccca cgccgttcca
     2101 cggcgtgtcg atgatggcca cgtccaccgc ctgctgctcg aagaagggcc ggtagtcgcg
     2161 ccggccgaag aggcactcgc aggaagccac cgggatcgtg gtgcggctcc gtacgtaggc
     2221 cagggccttc gggtcccgcg tgtccatctc gacccagagg aggtcgtagg gctccatggc
     2281 ccacgccatc ttgaggaaac cttcggtctt gtagttgaag ttgaggtcca ccaggatgtc
     2341 cgtgccgggc ccggcccctt cgcggaaggc ggcgagctcg gcgcggaggg cgtcgagcac
     2401 gtggcggtcg gcgttgagct cggggaagcc ggcgctgcga ccgaagccgg gcacgtgcgc
     2461 gcgcgggttg tcgcccagga gcagcacgtt ggtcttgagc gcggtgtagc ccttggccac
     2521 gacctcgcgg cccaccgcca cgatgtcgtc gagggtgcgc accggcggga tctgcatctc
     2581 ccgggcacgg ctcacccggt aggtggcgca gtgcgaccag tagagccgga tgcggtcgcg
     2641 gagcgggccg ccgaagaggg cgtagaccgg cacgtcgagg gagcgggcct tgatgtcgag
     2701 cagggcgttc tcgatggcgc cgatggcctg ctggatcacc ccgcccgtgg cctgccgccg
     2761 caccgcggac agcagggcca cgtgagcctc ccacgggcgc gggtctcggc cgatgagcat
     2821 cggcgcgagc ccggcgatgg cggcggagac cccgacgccg ccgaaggact cgttatactc
     2881 gctccacccg acgatgccct cgtcggtgct gatcttgagg aagtcgaggc tgcgccagcc
     2941 cccatcggca tgaagggact ccaccttcgt gatcttcatc gcgtctcctt ggtccgcccg
     3001 gtgagagagg ctcgggccgg ccggggtagg ctaccggagc gtcgagcagc tcggcaagcg
     3061 gtggcgcggc ggtccggccg ggcggtgttt ctcctgatgg tggggctcgt tggcgcggcc
     3121 tgcctcggac cggttcgcgg gctctacccc ccgcaacccg gcgacgccac tgtctccgtc
     3181 cacgtcgtga ggcacggctg gcacagcggg ctcgtgatcc ggcgcgatca gatcccgatg
     3241 ggggcgtggc ccgagctcgg ccggttcccg gccacgcgat tcctggaagt ggggtgggga
     3301 gatcgcgcgt tttaccagtc gccggacgcc ggggtcgcgc tggccatcga ggcctccttc
     3361 gcctcggagg gaagcgtgct ccacgtgacg ggcctcgacc ggcccccggc cgagcacttc
     3421 gctcacgccg agatcacggc ggtcgagctc tccgcgcgcg gcgcggagtc gctcgcccgc
     3481 ttcgtcgggc gcgcatacgc gcgggacccc gcgggcgctc cgatcgacct cggcccgggc
     3541 ttctatcctg ggagccgctt ctacgccgcg accggccggt actcgctcgt ctatacgtgc
     3601 aatagctgga tcgccgacgc cctccgggcg ggcggctgtc ccatcacgcc cgcctgggcc
     3661 ctcaccggcg gcaacctcgc cttccaggcc cgccggtgcg ggccgcccgc tcatcggtat
     3721 ccttgaccgc acgaaattcg ggcagttact ctcggctaga ctgccgggag gctccggcct
     3781 cgccgggtgg ggggaggcgt gatgagcggg tcgcttgcca cggtaccggg gcgggaggtg
     3841 tgcgttgcgc gctatctggt gcaacgcaac gcggcggagc acggcgaccg gccgttcctg
     3901 gtattcccgg acgccccgac gctgacctgg cgcggcgcgc atgatcgcgt cgtgcagatc
     3961 gcctgcggct tggcgcggct cggcgtccgc cagggagagc acgtgctcag ctggctgccg
     4021 accggcaccg acagcatcct gctcatgctg gccctgaacc accttggcgc cgtctacgtg
     4081 cccgtgaacc tggcctatcg tggccggctg ctggagcatg tcgtccgcct atcggatgcc
     4141 aggctgatgg tggcgcatgc cgacctggtg ccgcgcctgg aggagatcga tctggcccgg
     4201 ctcgaggccg ttgtcgtcct cggcggcgcc gcggcgacgg ggcggctcgc ggggcacggc
     4261 cccgaggtgc tggccccgag caatcgcgag ctgccgccgc tcccgcgccc catcgagccg
     4321 tgggacaccc agaccgtgat cttcacctcc gggacgaccg ggccatccaa ggccgtgctg
     4381 tcgtcctact gccaggggtg gtcgatgatg gggccggagg cctggccctt catcaccggc
     4441 gccgaccgct acttcatgac catgccgatg ttccacgtcg gcggcaccgg cctggtcaac
     4501 tgcatgacct tccgcgccgg ctcgctcgcc gtggtcccga gcttcgacac cgagagcttc
     4561 tggcccctgg tgcgccgcac cgaggcgacg gtcgtcttcc tgctcggcgc catggcgagc
     4621 ttcctgtgga aacgcccgcc cgggccgggg gatcgcgacc acaagctgaa gctcgccttc
     4681 atggtgccgc tggtcgacga cgtgccgggg ttcgccgccc ggttcggcat cgacgtgcgc
     4741 accgtgttca acatgacgga gatcaacaac ccgatcgtgt cggagccgca gcctcagggc
     4801 cgcggcttct gcggccgctt gcgccccggc agcgaggcgc gcctggtcga cgccaacgac
     4861 tgcgcggtgc cgccgggcga ggtcggcgag atgatcctgc gcacggcccg gccctgggcg
     4921 ctcaaccacg gctatcacaa cgacccggcg gccacggcgc gtgcctggcg caacggctgg
     4981 ttccacaccg gcgacgcctt ccgccaggac gccctaggca actactacta cgtcgaccgg
     5041 gtgaaggatg cgatccgccg ccgcggcgag aacatctcct cctacgaggt ggagatcgag
     5101 ctcttgtcgc atcccgcggt gcgggaggcc gccgcggtgg cggtgccgag cgagcacggc
     5161 gaggacgagg tcatggccgt gctgtcgctc gtgcccggcg ccagcctcga tccggcggag
     5221 ctggtcgcgt tcctgcgtcc gcggctggcg catttcatga tcccccgcta cgtacgcatc
     5281 accgccgagc tgccgaagac accgacgcag aaggcggaaa agcaccgcct gcgcgccgaa
     5341 ggcgtgacgc cggacacctg ggaccgcgag gtggccggca tccgcgtccg gcgcgagaag
     5401 gtcggcgggg tctgaggcgc aagctcctca gtagcggtag tggtccggct tgtaggggcc
     5461 ggtcttctcc acgccgatgt agcgggcctg ggcgtcggtc agctcggtga gactcgcgtt
     5521 cagcttcttg agctgcaggc gcgcgacctt ctcgtcgagg tgcttcggca gcacgtagac
     5581 cccgatcggg tacttctcgg ggttggtgaa gagctcgatc tgggcgatga cctggttggc
     5641 gaacgacgag ctcatcacgt agctcgggtg gccggtgccg cagcccaggt tcaccagccg
     5701 gccctccgcg agcaggatga tccgcttgcc gtcggggaag atcacgtggt ccacctgggg
     5761 cttgatgttc tcccacttgt actgcttgag tgacgcgatg tcgatctcgc tgtcgaagtg
     5821 gccgatgttg cacacgatcg cgttgttctt catccgcttc atgtgctcgt gggtgatcac
     5881 gtggtagttg ccggtggcgg tcacgaagat gtccgcctgc gaggcggcct cctccatcgt
     5941 caccacgcgg tagccttcca tcgcggcctg cagcgcgcag atcgggtcca cctcggtgac
     6001 ccacacctgg gccgacagcg cgcggagcgc ctgggaggag cccttgccca cgtcgccgta
     6061 gccgcacacc accgcgacct tgcccgccac catcacgtcg gtggcccgct tgatcccgtc
     6121 caccagcgac tcccggcagc cgtagagatt gtcgaacttg ctcttggtca ccgagtcgtt
     6181 gacgttgatg gccgggaact tgagccggcc ctccttggcc atctggtaga gccgcttgac
     6241 tcccgtggtg gtctcttcgc tgaccccacg gatcttgggg agccgggtgg agtaccagtt
     6301 cgggtccttg ccgagcttcg ccttgatcgc cgcgaagagc gcggtctctt cctcgttggc
     6361 cggatgcgcg atgacggagg cgttcttctc cgccgccgcg cccaggtgga gcagcagggt
     6421 ggcgtccccg ccgtcgtcca ggatcatgtt ggagtacgcg ccgtccggcc actcgaagat
     6481 gcggtgggtg tagtcccagt actcctcgag cgactcgccc tttcgcgcga acaccgcggt
     6541 gccctcggcc gcgatcgcgg cggcggcgtg gtcctgggtc gagaagatgt tgcaggaggc
     6601 ccagcggacg tccgcgccga gggcctgcag ggtctcgatc aggaccgcgg tctggatcgt
     6661 catgtggagc gacccggtga tgcgggcgcc cttgagcggc tgctggctcg cgtactcctc
     6721 gcggatggcc atgagaccgg gcatctcggt ctcggcgatc tggatctcct tgcggcccca
     6781 gtcggcgagg ctcagatcgg tgacgacgtg gtcctggggc cgggtcgatt tggtagtggc
     6841 gctcatctcg cgccctcctt atggtaaggg agtttgcgag cgccgttcgt cacagccgac
     6901 ccggggggcc ggccgctccg agcctggcgg gaagggctcc cgtcgcaacg ctcctcggag
     6961 ccgtgcaacg gacacgatta tacatgatag gaggaggctc acaatgctga ccgcggcgga
     7021 cttcatccgg gcgcagctca agcggcttca caccgggctc gaccaggcgc tggccgagct
     7081 gacgccggag cagctgcaca cggtgccggc cgggcatccg aaggccaaca cgatcgggtg
     7141 ggggatctgg cactacgtgc gcaccgagga caacatcgtc cgctgggtga tccaggaccg
     7201 gcggccgccc atctggaccg agagcggcta cggccagaag ctggggctgc cgcccgccgc
     7261 gcagggcacc gggatgtcca ccgaggaggc ccaggcgctc cgcatcaacg acatcccgct
     7321 cttccaggag tacacgcgga aggtgtgggc cggcaccgag gagctcttcg acgccgccac
     7381 gcccgccctc ctcgagaaga tggtgatggt caagccgctc ggcgagatgt cggtcgtcca
     7441 gtgcctgggc caggtcgggc tcacccacgg catgacgcac ctgggggaga tcgggctggc
     7501 ccggaccctg atcgccgggt cctgaccgat cgatgcgctt cggctggttg accctctcgc
     7561 attccccgtc ggcggccgac gatcacacgg ccatcgagga gcagctcacc caggcgtgcc
     7621 tggccgagga ggtcggcttc gactcggtct ggctcaccga gcacaacttc accggcgagg
     7681 ccgtctactg cgatccgatc ccgttcgcga gtgcgctggc cgcccgcacc acccgcgtgc
     7741 gcatcggctt tgcggtgatc cagctcgcgc tccggcatcc ggttcgcctg gccgtgcagc
     7801 tcgcgctgct cgataacctg agccgcgggc gcctcgacgt gggggtgggc cggggcaccc
     7861 tctacaacga atacgagtac gtgggttacg ggctgcgcag cgacgacagc cgcgagcgca
     7921 gcgcggaggc cctcgagatc ctgacccgcg cgtggaccga ggagcccttc agctaccagg
     7981 gcaagtactt ccaggtgagc ctgcccgcgc tgcgcccccg cgtgtaccag cggccccacc
     8041 cgccgatctg gcgcagcgtc gtgacgccgg gctcgttcgc ggagtgcggc cggcaggggg
     8101 tgcccatcct gacctcgcgc ctgccgctgg gcgtgctggg cgaacggctc aagctctacg
     8161 aggacggcct gatcgcgggc ggacatcctg cgccggtccg tgaccggctc cgccgcgatg
     8221 tcgcggtgtg gcgccacgtc tacgtgggcg agagccacgc cgaggcggag gaccggctgg
     8281 ccgacacgct cgcgcacacc cgccggcaca tgctcggcgc ccgcgccgcc cacaatcccg
     8341 aggactaccg ggtggatccc gcggtgctga acgcgttcaa cgatccgggc gtgtcggagg
     8401 acgaggcggt gcgccagtcg atggccaccg gcgcgctcta cggcaccgcc cggcaggtgg
     8461 cggagcggct ggccgagctg cgggccgccg gagtgcgcca cgtgctctgc cagatgagct
     8521 tcggctacct ggggcacgac cgcatcgtgg actcgatgcg gcgcttcggc cttcaggtga
     8581 tgcccgcctt ccgcgactac tagacgtact tcgcgaacca gtccaggatg atccggaagc
     8641 gctcgaggcg gtggcggggc ttgcccgagc gcgagagctc gtggttctcg tccgggaagc
     8701 gcacgagcac cgcgacgcgc cgcagggtct tcaacgccat gaagagctgc tcggcctccc
     8761 cgatcgggca gcgcaggtcg tcctcggagt ggatgatcaa cagcggggtc gtgatgtccc
     8821 gggcgtaggt cagcggcgag cgctcgaggt acttgcccat gttctcccac gggaaaatac
     8881 cgcccagctc tcccacgttg aagaggtggc cgatgtcgct cgacccgaac atggtgacgt
     8941 ggctgttcac cgcgcgctcc gagcaggcgg ccttgaaccg ggtggagtgt cccacgatcc
     9001 agctcgtcat gaacccgccg tagctgccgc ccagcaggcc cagccggccg ggatcgatcc
     9061 agtcgtaccg acgcagcgcc tcgtcgaggc cggcctgcac gtccgccgcg tcgccgccgc
     9121 cccagtcgcc caccaccgcg cgggtgaacg cttccccgta tccctggctg ccgcgcgggt
     9181 tggtgtagag gaccgcgtaa cccgcgcccg cctggacctg gaactcgtcg aggaacgggt
     9241 agccgtactg ggcgtgcggg ccgccgtgca cgttgaggag caggggatag cgcttgccgg
     9301 cctcgaagcc gaacggcttc atgatccagg cgtccaccgt catgccggcg cgctcgaaac
     9361 ggaagcgctc cggacggctc agcgcgacct cccccttcca gtcccggttg caggtcgtga
     9421 gctgcttctc cgcgccgccg tccgcgtcgc acacgaagac ctcggccggg gcatcgggcc
     9481 cgctcgcggc gaaggccacg gtcttgccgt cgcgggacgc ggagaagcca gcgacgaccc
     9541 gctcgccgcc gacgatctgc gtggcgggcc cgcccgcggc cgggacgcgg tagacgccga
     9601 ggctgccgtg gtcctccgca gccacggtga tggcgctgcc gtcggggctc cagagcgggg
     9661 gcaccccgag aggcgagcag gagcgatcga aggcggcggt gagacagcgc gacgcgccgc
     9721 ccgtcgccgg catcgcgaaa agccggatat tgttcccgaa cgcgttggcc tcggaccgcc
     9781 ccaggtaggc gatcgtgtcg ccggcgggcg agaaggcggc gtggccggcg gggcccgcgg
     9841 tgggggtcag gcggcgcggc gggccgccct cggcggccac gagccagatg tcggtggcgt
     9901 cgtcgtggtc gcggtcgtcg tggcgggccg acgtgaagac gatcgagcgc ccgtccggtg
     9961 cccacgcggg ctcggcgtgg tcgtggtcac cctcggtgag ctggcgcggc tcgccgccgt
    10021 ccgcggccac cacgaagatc tgcgggcgcc ggtcgtaggt gaagccctcg ccgttccatc
    10081 ggtagttcat ggtggtgatc acgcgcgggg gcttcgacct gcgcttgtcc tcctcggaca
    10141 ccggctcggg cgggccaccc acccgcgccg cgaaggcgag ccgcgtgccg tcaggcgacc
    10201 agacgggcgc gctcaggccg tgccggagat cggtgagacg gatgggctcg cccccggcgg
    10261 ccggcatcac gtggagctgc cccttcttgc ccggctcgcg ctcggagatg aaagccaggt
    10321 gcgacccgtc gggcgaccag cgcggggagg tgtcccgtcg cgggccggtc gtgaagcggc
    10381 ggggctcacc gccggtcgcg ttcaccaccc agatgttgga gagatactcg tcctgctcct
    10441 cggagagcgt ggtgaccacg aaggccaccg tccggccgtc cggggcgatc tgcggatcgc
    10501 tgacgaagcg gatgcgggtg agatcctgcg ggaccatggc gcgcggggtg ggcatggggc
    10561 gcatgatccg ccagggcccc acttcttgac aagcggtgag gccgcgccgt atcgtcccgc
    10621 tgccgatacg gttcgcgcta tccggtccga tcctcgttgg ctcctcaaag gggaggtcca
    10681 ccatgcggcc gttcatctcg atatccctcg cggtcctgct cgcgctcgcg ctggcatccc
    10741 cggcccctgc ccaggcgccg cctccctgga agcagggcca gcccgccgag atggccaact
    10801 cgccgctggc gcccatcgcc cagccgcccg cgcccaagcc tcccggcgag atcccggtgg
    10861 acaagatcaa ggtgccgcgg ggcttcaagg tgtcgctctg ggcccacggg ctcaacaacg
    10921 cccgggcgat gacctggggc gacaagggca cgctgttcgt cagcagccgc gtggccggca
    10981 acgtgtacgc ggtggtagac cggggtggcc agcgcgaggt caagaccatc gccaagggcc
    11041 tcaacctgcc caacggcgtg gccttcaagg acggcaccct ctacatcgcg gaggtctcgc
    11101 ggatcaccaa gatggcgggc atcgaggaca agctcgacag cccgcccgcg atggaggtgg
    11161 tctacgacat cctgccgcgc gatctcccgc acggctggaa gtacctggcc ttcggcccgg
    11221 acggcaagct ctacttcaat atcggcgcgc cctgtaacat ctgcatcccg cccgacaccc
    11281 atgccaacat ctcgcgggtg aatcccgacg gcaccgggtt cgagtacgtg gcgcacgggg
    11341 tgcgcaacag cgtggggttc gactggcacc cggtgacgaa agagctctac ttcgccaccc
    11401 acgcccgcga ctggctcggc gaggacgtgc cgagcgaccg cttcgacgtg gcgtcgaaga
    11461 agggcctgca cttcggcttc ccgtactgcc accagggcga catcctcgat cccgacttcg
    11521 gtcgcgggcg ctcctgctcg gagttcgcgc cgccgctgct caagaccggc ccgcacgtgg
    11581 ccggcaacgg cgtcgagttc tacaccggct cgatgttccc cgccgagtac aagaaccgcg
    11641 tgttcctggc ccagcgcggc tcctggaacc ggacgcagaa gagcggcttc aaggtgatga
    11701 tggtcacgct ccgcaccggc gacgtgcccc gctacgaggt cttcgcggag ggatggctcc
    11761 agggcgatca ggtctggggc cggccggtct acacccggca gatgaaggac ggctcgctgc
    11821 tgatcgccga cgactacgcg ggagcgatct accgggtcac ctaccagccc tgagccggct
    11881 gcgcttgctc gcgggcctct cgctcgccgc ggtcgtcgcg gcggcgggcg gggccccggc
    11941 gctcgccgcg gacctcgagg cagggcggcg caaggccgag ccgtgcgcgg cctgccacgg
    12001 ccgcgacggc cacgcgaccg ttccgggcac gccgtccctc gccggacagc ccgtctactt
    12061 cacgcactgg cagctcatca agtaccggga cggccggcgg aaggaccccg tgatggcgcc
    12121 cctcgccgcg acgctgagcg acgcggacat ggccgatctc gcggcctact acgccgcgca
    12181 gcgtcccacc cggcgtcccg cgatcacgga tccggcgcgg gtcgcggccg ggcggccgct
    12241 cgccgacctc taccactgca gctcgtgtca tcgccccggg ttcgtcgggc aggagcaggc
    12301 cgcgcgcctg gccggccagg acctcgacta cctcctgcgc ctgctccgcg ccttcaagag
    12361 tcgcaccgct tccgatctcg acgggaccat gacggtggcc acgcagccgc tgaccgacga
    12421 cgagatcgtg agcctggtcc actacatcgc cacgctcggc gagccctagc gccgacactg
    12481 gtggacatgc ggaccacgct gagggagccg ttctcgcagg accgggcggc cgtccagagg
    12541 gtgttgttgc gctcctgctg cgcgggcgtc aaggcgacgc acgccgcggc gagcccggcg
    12601 gcgagcgtgg cgacgaggag ggatccgacc atgcgcggga cctcctgccg cgccatggtg
    12661 ggacgtcggc tacgcccagt caatgtgtag gcgcagaaac tctcatgggg gacaataccg
    12721 gatagggagg acccgcatgc atatcgtgat ggtgacgctg atcgcgctgg tggccgccgc
    12781 gcccgccgtg gcgcaggcgc aggtgcaggt gcacgtcgac attggcctcc gtctccccgc
    12841 tccgcctcgt ctggtcgtcg tgccccaggt gcccgcggtg cgttacgtgg cggagcccgc
    12901 cgctcccggc aacctgttct tctacagcgg gcaatactgg gccttcgcga gcggcggctg
    12961 gtacgtgagc ggcggctaca acggtccatg ggtcgtcgtc gcccccgaat tcgtgcctcg
    13021 acccgtgctg ttcgtgccgg tgcagtacta ccatgtcccg cccggtcact ggaagaagtg
    13081 ggcgcctcag cggcctccgc gctggcagga ggattggggg cgggagtggg ccgacaagcg
    13141 cgagtggaag tcgcgtgacc gcgaccggga cgacgacgat cgggacgacc ggcgaggcaa
    13201 gagcaagggg aaagggaagg ggcacgggcg ctagcccggc cgcggccggc tacaggtacc
    13261 agcgccggaa gatctcgaag acgttcagct cgccgccccg ccagtagcga ttcgcggaca
    13321 gcgagaaggc gcggcgcttg cggtcgtacg tgaggcgggc cgggcgctcc ggatcgttcg
    13381 ggtcgtaggc gaggaactcc gccccggcgc ccgtgcgcgc gccctcgaac acgaccatcc
    13441 cgtggttgat ggccagcgag gggaagcgca gcaggtgcac gagcggcgcg ccgtgatcct
    13501 cgactgcggc cagcagctgg cccgcggtgc ggtgctgatg cccgcgcgag accgggacca
    13561 cgatccgcca gtggctgtgc tgcacgtacg accgccacgc cccgccgcag gccgccttca
    13621 ccaccggctc gcgcgccgcg ctgagctccc ggagcccggt gtagcccggg atgaccacgc
    13681 gctctccggg cggagacggc cgccgcggag gccgggcgag caccgcgcgg atcagtcgcc
    13741 gataggtcag gtcgtcggcc ggctcgatcg tcggctcgaa ccgtgcgtgg tagaagaact
    13801 gccgcgcggc ccggaccagc acgaagcagc ggagcgcgta gcggggcgcg ggctcgcggc
    13861 ggacgacgcg ggtctgcccg gtcgtcgcat cgaagtggta ctcccacacc agctcgttcg
    13921 ggaacgcgaa cgtgtcccgc tcgaagtcga agcgtcgggc gagcgcgagc atgacgtcta
    13981 gagcctacac ccgccgcccg cgccggtcat tacctcgtgg gtaattgcgg ccgggagcgg
    14041 cttcgccgac attgagccag aaaggagatc acccatggtc aatgcaacgg tgaaggagct
    14101 gcagcggaag aacgacgcgg cgtgggaggg cctcgcccgg acgctcgagg gcatggaggc
    14161 gcacctcgag cgggccgaca cgccgggcga gtggacggtg cgccaggtgc tctgccatct
    14221 gctcttcgag ccgggctcga agccggtcgc cctgctcgag cgcttcgcga tcggcgaccc
    14281 gcccctcgtg gcgatcacgc cgggcgcggt gacggtgacg cccgaacggc agcgcatgac
    14341 gctggccgac ctggtcgcgg ctctcgacgg ccagcggcgg gacgtgttcg ggcacctcgc
    14401 gacgctcggc gaggcggacc tcgcgcgccg agcccgcatc cccctctttg ctccgcttct
    14461 cggcaccgat gaggtcccgc tgcccgtctt cgtcggcatc atgttcgacc ggcactggac
    14521 cgcgcacacc gagcagctcg cgaagatccg tcgcgcggcc ggactgcccg aagcgaagta
    14581 cactacctcg acgacgggag cgatacgatc agcacggacc gcacgacatt tctccgccgc
    14641 ggtctccagc tcgacggggg gctcgcgcgc ctgcgcgaga tggcggggtg agcgcggcgc
    14701 cggcccatcc cgcgccgggc tggctcgcct gccgtgccgg cacccgcttc gtcatcggcg
    14761 gctaccacga ggaggatttc gagatctggg actcgaccgg cgccctcgtg gcccagcccc
    14821 gccagctcgc gctgctgccg tagcggccgg cgtccggtgt cgcatcaaga tgttatgatc
    14881 cgcgaacatg gcggatcaga cggacgacat cgcggggcgc atcgtgctga tcgtggatgc
    14941 gagcgtgccc gccgccacgc gggctgcctg cgacgatgcg gccgcgacgc tcgcccggtg
    15001 cgcggggatg ccggtgacgc tggccgtggt tcccatcgcg acggtggacg cggtccagcc
    15061 cgtcatccgg cgcggccccg gtcagattcg cgacctcgag ccgccgtcgg tcgcgggcgc
    15121 cggggacgcc atgacgtcgg ccccattcgt gtggcgccgc gacggccggc cggactgggg
    15181 cgcgatgtgg accacgttct gcgatctggc gctccacggt ggcccgccgc accgcggcgc
    15241 gctccaggcg ctgcgcggac cctctatcgg agatcgcgac gccgtgtcgg cgcccgagat
    15301 ctcggccgag ctccggcggg ggatcgagga gacgaccggc ctcgccacgg cgcccgccga
    15361 gcccgggtgg atcgcgatca cgtgcgcgag cgcccgcatg gcggcctggc tctgcgccac
    15421 catcatcctg gagaacgtgg acgcccgcgt ggagggcacg cgactcctcg tgcccgccgg
    15481 cccggggttc gcgctggagg accaggtcaa gagcgtgatc acggtggtgg cgaagacgca
    15541 tcactactgg cgggagcacc acgaatcggc gcgggagccc cgatcctgat cgcggccctg
    15601 cctcccgcgc ccgaggcgcg agagctggcc gggctcgagg cggacgtggt cgagctgacc
    15661 gcgcaggagc gccgccgcgc ccgccgccgc gtggtcactc gcgccggccg ggtcttcacc
    15721 ctcgcgctcc ccgccggcga cgtgctcgtt cccggccgcg tgctgcacgt gggccccggg
    15781 tggtacgtga cggtggaggc cgcgccggag gcgctgctgg cgatcacgcc gcggactccg
    15841 cgcgaggggc tgcgcgtcgc cctggaggtg ggccggctcc acgcggccct cgcggtggcc
    15901 ggcggccgcc tgctcgtgcc cgacgagccg gccgtggagc ggctgctccg gcggctgggc
    15961 gtgccgtggg tccgcgcccg cgagccgttc ctgcccgtgt cgtccggctc gccgcactga
    16021 ggccgctccc cgcagatcgg gcgtgacaag ccggaccgct tcgactaagg tgctcgtgcc
    16081 gaatgcggaa tggtattcgg taatgtgaat gggcgactcc agagggggag gggccaccgg
    16141 aagatgaccc gggagcggac gatccgaggg caggtcgcgg tcgtcggggt cggggagacg
    16201 acctactaca agcacggccg ggcgcccgag tccgagttcg cgctggcgct gcgggccatc
    16261 ctccgcgcct gcgccgacgc gggcatcaat ccccgtgaga tcgacggctt cgcctcctac
    16321 agcaacgacc gcaacgatcc ctcgcgcctg gccgccgccc tcggtctgcc cgagctgcgc
    16381 ttctcgaaca tgcagtgggg cggcggcggg ggcgggggct cggcggcggt gggcaacgcg
    16441 gcggccgcgg tggccgcggg ttacgccgac tgcgtcgtcg tcttccgcgc gctcgcccag
    16501 ggccagttcc agcgcttcgg cgccgcgccg cccggcggca cggtgtccgg cgaggcggcg
    16561 ctcacgttcc cgtacgggct catctcgccg gcccagcgct tcgcgatgcg ggtgatgcgc
    16621 ttcatgcacg accacggggt gcggccggag gcgcagcggg ccatcgcgct cgcgtcctat
    16681 catcacgcgc aggcgaaccc tcgcgccgtc atgcacggcc ggcccctgac cccggaggcc
    16741 tacgacgcct cgcgcttcat cgtcgagccc ttccgcctct tcgactgctg catggagaac
    16801 gacggcgccg ccgccctggt gctggtcgcg gccgagcgcg cgcgcgacct gcgccggccc
    16861 ccggcctacc tgctgggcgt ggcgcagggc tcggagtacc gcaacgcggc gcgcggccac
    16921 aacgctccga cctacgcgtc ctcgagc
//
LOCUS       DASUFA010000442         6738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_341921, whole genome shotgun sequence.
ACCESSION   DASUFA010000442 DASUFA010000000
VERSION     DASUFA010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341921"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="VFX87_16085"
     CDS             complement(<1..1672)
                     /locus_tag="VFX87_16085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX4996500.1"
                     /translation="MPELRSPRVWLAMVWSIFQLYTAYAGLYDLLIQLPVHVAFAIAL
                     GFLTEPTPDSPEAAARLAAHRPRRWLDGALALLALLCAAHFVWHNERLASRMAMVDDP
                     ERLDVVVGVLFTALLLEAARRHIGPALVVLALAFVAYAFAGPWLPGFAGHGGETFLKL
                     VDQQTLTTQGIFGIPTLVSATFIFLFVVFGSVMSHGGLLRFFTDGALAVAGGSRGGAG
                     KVAVIASGLFGTVNGSAIANAVTTGAFTIPLMKRAGYRPEFAAGVEACASMGGQLIPP
                     VMGAAAFIMAETLQVPYASIAVAAAIPGVLYFVAVAVMVHFEAARRGLPVLPRSALPR
                     LGAVLRRDLHLLGGPAVLVYFLVDGRSPLFAGFWGLIAAFALSWVRRDTRVGLVRSLA
                     ILSDSAHAAMPVALACATVGIVVGVVSTTGLGLKLATGIVGLAGGSLLLTLILTMVAA
                     LVLGTGLPTSATYIITSIMAAPALVELGVPKLVAHMFVFYFGILADLTPPTAISTYAT
                     SSIAGADVWRTQWLAMMLALSGFIIPFSFAYDPALLMLDAGLFHIGWRT"
     gene            complement(1665..3332)
                     /locus_tag="VFX87_16090"
     CDS             complement(1665..3332)
                     /locus_tag="VFX87_16090"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4996501.1"
                     /translation="MDEIADGLVLVNGRILTLDPRRPVVAALAVAGGRVAATGGRGEV
                     ERRRDRHSRVIDLRGATVIPGLVDAHAHLDREGLKLIYPSLARCRSIADIQRLIRAHA
                     ARRKPGEWIVTMPVGAPPFYQGVPDCLADRRWPTRADLDAAAPDHPVYIRGIWGYWNR
                     PPVYSVANSRALALAGITRETSPPPGVEIVKDAGGQPTGVFVEHNLIQVLEFTLMRAA
                     PRFTPDDRLRALRASQRRYAARGVTAVYEGHGIAPEVLAVYRESHARGEMRLRCTLAV
                     SPTWDAAEAARAIPGLAAWAGGRGLGDDRLRVGGICLHYGGDAGVARILHAAQPYTGW
                     AGFVESANDPAAYLEQAELAARHGLRVNTLVTRCLPEVLAAWEDVARRHRIRDLRWVL
                     VHLNAATPEQLARIRRLGAVATTNPISYLYRSAGEEVAKLGGAADQLLPHRSLARHRI
                     PFGIATDNKPADPWVAFAAIVARRDMRTGVVVGERERLTRAQALAALTQGGAWVTFAE
                     RERGRLAPGWAADLAVLEHDPLTAPLEALVGQTARLTLVGGEIVHHA"
     gene            complement(3320..4303)
                     /locus_tag="VFX87_16095"
     CDS             complement(3320..4303)
                     /locus_tag="VFX87_16095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX4996502.1"
                     /translation="MRMLLAVMLVVAVGVSTGAAQTPAPVNLAFATLDTGSAWYVYGA
                     TMAELLRKTLPPGSNIDVKPRAGGVGNPRLVAKNETPLGLSFTVTNRWAFEGKEAYTE
                     KLENIRALVGGFDTYYLVAMATKKLGITSVREIRDKKLPVRVYTQPVGSLGEFAGRQL
                     LRAVGLGYADIKGFGGSTQHVGYNVIIDAFKDGRADILIAVVTPKHPSVSEIVSSVDV
                     EFLGLDADTAKALLPLGYAAATMPPDTFKNQSKPVSTVGFPTVLITNKDLPEPIAYTV
                     TKTVVENKDALVRGHGGLAEFDPRTAWQPDKVGIPLHPGAERAYREKGWMK"
     gene            complement(4399..5490)
                     /locus_tag="VFX87_16100"
     CDS             complement(4399..5490)
                     /locus_tag="VFX87_16100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4996503.1"
                     /translation="MKGADVLVVGAGCVGANVAYRLAERGAAVTVLDGGAPGAGASGA
                     SFAWTNSFNKTPRDYHDLNVASMEEHAGLAKELGGGWLHQDGALAWEQGPAGRARLGQ
                     AVERLTGWGYAVERISPRQARELEPDLHIAPTVEEVVWTPGEGYVEAVPFIGALLAEA
                     TRRGARVLPGRRVTGVIRSGARVQGVSTEGGDRFEADVVLDCAGTAMDEIARLAGVAL
                     PIDRVPGRLIYTAPVATTLRRPIHAPGCHFRPDGAGRIVLAEGAHDQVWAEAATPWPP
                     ERSLATVAAHLPALEGARVEATRIGVRPMPRDERPMVGGIPGLDGFYVVASHSGVTLG
                     PLWGRIAAAELLDGTLDPRLSAYRPTRFL"
     gene            complement(5499..6134)
                     /locus_tag="VFX87_16105"
     CDS             complement(5499..6134)
                     /locus_tag="VFX87_16105"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4996504.1"
                     /translation="MSLRLGDTAPDFTAETTQGPVKFHDWIGDGWAILFSHPRDYTPV
                     CTTELGYVARLKGEFDKRHVKCIGLSIDPLDSHKGWMADIKETQGHEPKFPVIADPER
                     KIANLYGMMHPAHDEVYTVRTVFVIDPKKKIRLMITYPQTTGRNFDEILRVIDSLQLT
                     DGYRVATPVNWKPGEDVIIVPAVTDEEAKAKFPKGWKTLKPYLRLTPQPNK"
     gene            complement(6155..>6738)
                     /locus_tag="VFX87_16110"
     CDS             complement(6155..>6738)
                     /locus_tag="VFX87_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX4996505.1"
                     /translation="PDRRRTMATETYTLEQFVADLDRITTRETAPETITAQIAPLLGR
                     LVANPDAIPARFRGRAPDGGRGRFMLHRAPRFNVTAVVWRPGDRAAAHNHETWGVIGV
                     IDNEIEETRYRVTENAGGNAAVAVQSVTRHGAGAVSRLVPPTDEVHAMHNPTARDTVE
                     IHVYGKDLYGLHRKTWADDGRVSPLVSPKYVNC"
BASE COUNT          955 a         2337 c         2525 g          921 t
ORIGIN      
        1 ccgtgcgcca cccgatgtga aacaagccgg cgtcgagcat caagagcgca gggtcgtagg
       61 cgaagctgaa cgggatgatg aagcccgaga gcgccagcat catcgccagc cactgcgtcc
      121 gccacacgtc cgcgcccgcg atggacgagg tcgcgtaggt cgagatcgcg gtcggcgggg
      181 tgaggtcggc caggatcccg aagtagaaca cgaacatgtg ggcgacgagc ttggggacgc
      241 ccagctcgac cagcgccgga gcggccatga tcgaggtgat gatgtaggtc gcggaggtcg
      301 gcagtccggt accgagcacg agcgcggcga ccatcgtcag gatcagggtg aggaggaggc
      361 tgcccccggc caggcccacg atgccggtgg cgagcttgag cccgagcccg gtggtgctca
      421 cgacgccgac cacgatgccc acggtcgcgc aggccagcgc caccggcatc gccgcgtggg
      481 cgctgtcgct caggatcgcc aggctccgga ccagcccgac ccgggtatcc cgccgcaccc
      541 acgacagcgc gaacgccgcg atcaggcccc agaagccggc aaacagcggc gagcggccgt
      601 cgaccaggaa atacacgagc acggccggcc cccccagcag gtgcaggtct cgccgcagga
      661 cggcgcccag acggggcagc gcgctgcgcg ggagcaccgg cagaccccgg cgggcggcct
      721 cgaagtggac catcaccgcg accgccacga agtagaggac gccggggatc gccgccgcca
      781 cggcgatcga ggcgtacggc acctggagcg tctcggccat gatgaaggcg gccgcgccca
      841 tgacgggcgg gatgagctgg ccgcccatgg acgcgcacgc ctcgaccccg gcggcgaact
      901 ccggccggta gccggcccgc ttcatcagcg gaatcgtgaa ggcgccggtg gtgacggcgt
      961 tggcgatcgc gctgccgttg accgtcccga acagcccact cgcgatcacc gcgaccttcc
     1021 ccgctccgcc ccggctgccg ccggccaccg cgagggcgcc gtcggtgaag aagcgcagca
     1081 ggcccccgtg ggacatcacg ctgccgaaga cgacgaacag gaagatgaac gtggccgaga
     1141 ccagcgtggg gatcccgaag atcccctgcg tcgtcagcgt ctgctggtcc accagtttga
     1201 ggaacgtctc tccgccgtga ccggcgaagc cgggcagcca ggggcccgcg aaggcgtagg
     1261 ccacgaaggc cagcgccagg acgaccagcg ccggaccgat gtggcgccgc gcggcctcca
     1321 gcagcagcgc ggtgaagagc acgccgacga cgacgtccag gcgctcggga tcgtccacca
     1381 tcgccatccg gctcgccagc cgctcgttgt gccacacgaa atgcgcggcg cagagcaggg
     1441 ccagcagcgc cagggctccg tcgagccagc gccggggacg gtgtgccgcc agccgggccg
     1501 cggcctcggg cgagtcgggg gtcggctcgg tcaggaaccc gagcgcgatg gcgaaggcca
     1561 cgtgcaccgg cagctggatc agcaggtcgt agagtccggc gtaggcggtg tagagctgga
     1621 agatcgacca gaccatcgcc agccagacgc gcggcgagcg cagctcaggc atggtggacg
     1681 atctccccgc cgaccagggt gaggcgcgcg gtctgtccga ccagcgcctc gagcggcgcg
     1741 gtcagcgggt cgtgctcgag caccgcgagg tcggccgccc agcccggggc cagtcggccg
     1801 cgctcgcgct cggcgaacgt cacccaggcg ccgccctgcg tgagggcggc gagcgcctgg
     1861 gcgcgcgtga gccgctcgcg ctcgccgacc accactcccg tccgcatgtc gcggcgggcc
     1921 acgatcgccg cgaaggcgac ccaggggtcg gccggcttgt tgtcggtggc gatcccgaac
     1981 gggatgcggt ggcgggcgag gctccggtgg ggcaggagct ggtcggcggc gccgccgagc
     2041 ttggccacct cctcgccggc cgagcggtag agatacgaaa tggggttggt cgtggcgacg
     2101 gcgccgagcc ggcggatgcg cgccagctgc tcgggcgtgg ccgcgttgag atggacgagg
     2161 acccagcgca ggtcccggat gcgatggcgc cgcgccacgt cctcccacgc ggcgaggacc
     2221 tccggcaggc agcgcgtcac cagcgtgttg acgcgcagcc cgtggcgcgc cgccagctcg
     2281 gcctgctcga gataggcggc ggggtcgttg gcgctctcca cgaaccccgc ccatcccgtg
     2341 tagggctggg ccgcgtgcag gatgcgggcg acgccggcgt cgcccccgta atgcaggcag
     2401 atgccgccca cgcgcaggcg atcatcgccg agcccgcgcc cgccggccca cgccgccagt
     2461 ccggggatgg cgcgggcggc ctcggccgcg tcccacgtcg ggctgacggc cagcgtgcag
     2521 cgcaggcgca tctccccgcg cgcgtggctc tcccggtaga cggccagcac ctcgggcgcg
     2581 atcccgtggc cctcgtacac cgcggtgacg ccgcgggccg cgtagcggcg ctgcgaggcg
     2641 cggagcgcgc gcaggcggtc gtcgggcgtg aagcgcggcg ccgcgcgcat cagggtgaac
     2701 tcgagcacct ggatgaggtt gtgctcgacg aacacgccgg tgggctggcc gccggcgtcc
     2761 ttcacgatct ccacgccagg cggcggcgac gtttcacggg tgattccggc gagtgcgagg
     2821 gcccggctgt tcgcgaccga gtagaccggc gggcggttcc agtagcccca gatgccccgg
     2881 atgtagacgg gatggtcggg cgcagcggcg tcgaggtccg cgcgcgtcgg ccatcgccgg
     2941 tcggccaggc agtcgggcac gccctggtag aacggcgggg cgccgaccgg catcgtgacg
     3001 atccactcac ccggcttccg gcgggccgcg tgcgcccgga tgagccgctg aatatcggcg
     3061 atcgagcggc agcgcgcgag cgaggggtag atgagcttga ggccctcgcg gtcgaggtgg
     3121 gcgtgggcgt cgacgagccc ggggatcacg gtcgcccccc ggaggtcgat cacccgcgag
     3181 tggcggtcgc gccggcgctc gacctccccg cgcccgcccg tcgccgcgac gcgcccgccg
     3241 gcgacggcga gcgcggcgac gacggggcgc cgcgggtcga gggtcaggat gcggccgttg
     3301 acgagaacga gcccgtcggc tatttcatcc accccttctc gcggtacgcc cgctcggcgc
     3361 ccggatgcag ggggatgccg accttgtcag gctgccacgc cgtccgcgga tcgaactcgg
     3421 ccagcccgcc gtgcccacgc acgagcgcgt ccttgttctc cacgaccgtt ttcgtcaccg
     3481 tgtaggcgat cggctccggc aggtccttgt tggtgatcag gaccgtcggg aagccgacgg
     3541 tgctcaccgg cttcgactgg ttcttgaagg tgtcgggcgg catggtggcc gccgcgtagc
     3601 cgaggggcag cagcgccttg gcggtgtccg cgtcgaggcc gagaaactcg acgtccaccg
     3661 aggacacgat ctcgctcacc gacgggtgct tgggggtcac caccgcgatc aggatgtcgg
     3721 cgcggccatc cttgaaggcg tcgatgatga cgttgtagcc gacgtgctgg gtggagccgc
     3781 cgaacccctt gatgtcggcg tagccgaggc cgaccgcccg cagcagctgg cgcccggcga
     3841 actcgccgag cgagccgacc ggctgggtgt agacgcggac cggcagcttc ttgtcgcgga
     3901 tctcgcgcac cgaggtgatc ccgagcttct tcgtcgccat cgcgacgaga tagtaggtgt
     3961 cgaagcctcc gacgagggcg cggatgttct cgagcttctc ggtgtaggcc tccttgccct
     4021 cgaaggccca gcggttggtc accgtgaagg acaggccgag tggcgtctcg ttcttggcca
     4081 ccagccgcgg gttgccgaca ccgccggcgc gcggcttgac gtcgatgttc gagccgggcg
     4141 gcagggtctt gcgcagaagc tcggccatgg tggcgccgta gacgtaccag gcgctgccgg
     4201 tgtccagggt ggcgaaggcg agattgaccg gcgcgggcgt ctgggccgcg cccgtcgaca
     4261 caccgaccgc cacgacgagc atgaccgcga gcagcattct cattccgtct ctcctttggt
     4321 cggggaaccg tagggatcct gccagatcgg caggggccgg gtcaaacggc gcgtgggtgc
     4381 gcggcgccgc gccaatggtc agaggaagcg cgtcggccgg tacgcgctca ggcgggggtc
     4441 gagggtaccg tcgagcagct cggcggcggc gatgcggccc cagaggggac cgagcgtgac
     4501 gccgctgtgc gaggcgacca cgtagaagcc gtcgagcccg gggatgccgc cgaccatcgg
     4561 ccgctcgtcg cgcggcatcg ggcgcacccc gatgcgcgtg gcctccaccc gggcgccctc
     4621 cagcgcgggc aggtgcgccg cgaccgtcgc gagcgagcgc tcgggcggcc agggggtggc
     4681 ggcctcggcc cagacctggt cgtgcgcccc ctcggcgagc acgatgcgcc cggcgccgtc
     4741 gggacggaag tggcagccgg gcgcgtggat gggccggcgg agggtggtgg ccaccggcgc
     4801 agtgtagatc agccggccgg gcacgcggtc gatcgggagg gccacgccgg ccaggcgggc
     4861 gatctcgtcc atcgccgtgc ccgcgcagtc cagcaccacg tcggcctcga agcggtcgcc
     4921 gccctcggtg ctcacgccct ggacccgcgc gcccgagcgg atcacccccg tcacgcgccg
     4981 gccggggagc acgcgggcgc cgcggcgcgt ggcctccgcg agcagcgcgc cgatgaaggg
     5041 gaccgcctcc acgtagccct cgcccggcgt ccacacgacc tcttccacgg tgggcgcgat
     5101 gtggagatcc ggctcgagct cgcgggcctg tcgcggcgag atccgctcga ccgcgtagcc
     5161 ccagccggtc aggcgctcca ccgcctgccc gaggcgggcg cggccggcgg gcccttgctc
     5221 ccacgcgagc gcgccgtcct ggtggagcca gccgccgccc agctccttgg cgagcccggc
     5281 gtgctcctcc atgctcgcga cgttgaggtc gtgatagtcg cgcggtgtct tgttgaagga
     5341 gttcgtccag gcgaagctcg ccccggacgc gccggcgccg ggcgcgccgc cgtccaggac
     5401 ggtgacggcg gccccgcgct cggcgagccg gtaggccaca ttggcgccga cgcaccccgc
     5461 tccgacgacg aggacgtcgg cgcccttcat gcggggcgct acttgttcgg ctgcggggtg
     5521 aggcgcaggt agggcttgag cgtcttccag cccttgggga acttggcctt cgcttcctcg
     5581 tcggtgaccg cgggcacgat gatcacgtcc tcgcccggct tccagttcac cggggtggcc
     5641 acgcggtagc cgtcggtgag ctggagcgag tcgatcacgc gcaggatctc gtcgaagttg
     5701 cggccggtgg tctggggata cgtgatcatc agccggatct tcttcttggg gtcgatgacg
     5761 aacacggtgc gcacggtgta cacctcgtcg tgcgccgggt gcatcatgcc gtacagattc
     5821 gcgatcttcc gctcggggtc cgcgatgacc gggaacttgg gctcgtggcc ctgggtctcc
     5881 ttgatgtcgg ccatccagcc cttgtgcgaa tccagcgggt cgatcgagag cccgatgcac
     5941 ttgacgtggc gcttgtcgaa ctcgcccttg agccgcgcca cgtagcccag ctcggtggtg
     6001 cagaccgggg tgtagtcgcg cgggtgagag aagaggatgg cccagccgtc tccgatccag
     6061 tcgtggaact tgaccgggcc ctgggtggtc tcggcggtga agtcgggcgc ggtgtctccg
     6121 agtctcagtg acatgtgacg tcctcctgat gggttcagca gttgacgtat ttcgggctga
     6181 cgagtggcga cacacggccg tcgtcggccc aggtcttgcg gtgcagcccg tagagatcct
     6241 taccatacac gtggatctct accgtgtcgc gcgccgtcgg gttgtgcatc gcgtgcacct
     6301 cgtcggtggg cggcaccagg cgcgacaccg cgccggcccc gtggcgggtc acgctctgga
     6361 cggcgaccgc ggcgttgccg cccgcgtttt cggtcacgcg ataacgggtc tcctcgatct
     6421 cgttgtcgat gacgccgatc acgccccacg tctcgtggtt gtgggcggcc gcccggtcgc
     6481 cgggccgcca taccaccgcg gtgacgttga agcgcggggc ccggtgcagc atgaagcggc
     6541 cgcgcccgcc gtccggggcg cgcccgcgga accgggcggg aatcgcgtca ggattcgcca
     6601 ccaatcggcc gaggagcggc gcgatctggg cggtgatagt ctcgggcgcg gtctcgcggg
     6661 tggtgatccg gtcgaggtcc gccacgaact gctcgagcgt ataggtctcg gtcgccatcg
     6721 tccgtctccg atcagggg
//
LOCUS       DASUFA010000443         5234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_342389, whole genome shotgun sequence.
ACCESSION   DASUFA010000443 DASUFA010000000
VERSION     DASUFA010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342389"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..991
                     /gene="trpA"
                     /locus_tag="VFX87_16115"
     CDS             158..991
                     /gene="trpA"
                     /locus_tag="VFX87_16115"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX4996506.1"
                     /translation="MKRPAAAPRPASRLAATFAALRARRERALVAYFTAGDPSLAMTR
                     RLVGEAARRGADVIELGIPFSDPLADGPVIQRASQRALAAGVTLPRVLELVREMRGEV
                     SVPLVFLTYYNPILAFGLKAFCRTAVEAGIDGVIVADVPIEEAAPLRAEAEPAGLDVV
                     HLVAPTSTPERMRKIARASQGFIYMVSLTGVTGERSTLAPELAQQLRALRNITTTPIC
                     VGFGISTPEQAAEVGRAADGVIVGSAIVRLVEQYGTSAELLARVGDFIASLKAPLKGE
                     R"
     gene            1142..2011
                     /gene="accD"
                     /locus_tag="VFX87_16120"
     CDS             1142..2011
                     /gene="accD"
                     /locus_tag="VFX87_16120"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX4996507.1"
                     /translation="MSAWFWKKKDGDGDERPKKVVIAEGLWVKCDSCKEIVYRAEVER
                     AGRVCPKCGYPFRISARERIALVADDGSFEEREAGLRSKDPLNFKDTKKYTDRLKAAR
                     SKTDAGEAVVTGTARIGGYPVAIAVFEFAFLGGSMASVVGEKLTRTIELALQKRLPLL
                     IISASGGARMQEGILSLMQMAKTSAALQRLDAERIPYISLLTDPTTGGVTASFAMLGD
                     LILAEPRALIGFAGPRVIAETIRQPLPDGFQRSEFLLEHGQLDLVVERRELRATLRRI
                     LAFFSGRAPTPTP"
     gene            2026..3300
                     /locus_tag="VFX87_16125"
     CDS             2026..3300
                     /locus_tag="VFX87_16125"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX4996508.1"
                     /translation="MARILALRGGERTGMRPGLERIEAMLDAIGRPEQAFTIAQVGGT
                     NGKGSISAMLAAILQAAGRRVGLYTSPHLAHYRERIRVNGRPISDSDFVDGVDALGTL
                     ITRLDLTVFEAGTALALDHFCRERVDVAVLEVGLGGRLDATTVGKPRVVVLGSIDYDH
                     QHELGDTLALIATEKAAIIRSGVAFSARQDPEVEAVIIRRAAEAGVPLSLEGRDLRVT
                     PLGFTLEAQRLDLEGPDWRIPDVSCGLLGVYQPGNALLAAAAARELGADEGAIRAGLR
                     GARWPGRFQIFRRDPLVILDGAHNPAGARALAASLRAYFPGRPVTFVIGVLADKDAGG
                     ILAALRPLAARIILTAAANPRAAPPDALQALLPAGTRVATARSPQAALAMATAEDPHG
                     IVCIAGSLSLIGDVLAVAGDGQDVLFTAGARC"
     gene            3342..>5234
                     /gene="lptD"
                     /locus_tag="VFX87_16130"
     CDS             3342..>5234
                     /gene="lptD"
                     /locus_tag="VFX87_16130"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX4996509.1"
                     /translation="MSGPSGLARGTRMFVFVGLLTALLACVELSAGPAWAQPGGTTVR
                     AGGEEATVIADQLQQVGGATDLLIAIGNVEIIRGQTRLLADRVELNRDTGQAVAQGKV
                     VFYDGPDRLVGERIDYNLKTGTGVVYQGSAVSAPFYHLSAEQMDRVGDSVYEMRRGVF
                     TTCEGDDPPWSFRFGSGTADLDDIVYGRDASFWVNGVPVLPWVPFFAAALRRERQSGF
                     LFPEFGQSSRKGVFSKIPYFWAIDDSQDLTVSLDAYSRRGVGLEGDYRYVMSREQRGE
                     LTLFGVDEFLRDDPRLQENRGWASFRHDWQITPRLSFKVNANVTSDDLIFRDYGNRLA
                     ERATQRAETNVFVTQNWDAWSLVGNVKWYQDLTTPRPIELQRVPEIKVVGIRQPVPGV
                     PGLLYETTASFVNFIRDVGPDGIRADFHPRLLMPLPVGGVFTVTPFAGGRLTYYDERV
                     VDQRLTRVGALSVEKSVHDPRVRRQVEWGAEAESRVSRVYTSAGGSGVAAYQHVIEPR
                     VRIIQIRGVEQKDFPQYDPGASTATGIDPGYERRVGIDRVGKANEITYYLTNSLNAKT
                     VAGPDQLPVRWEMVRFTLSQTLKMDPVSQPLKDLYGDLTVSPNQRIGFHADARYNVYD
                     LGLREAN"
BASE COUNT          729 a         1871 c         1846 g          788 t
ORIGIN      
        1 agtcctcgca cgcggtggcc cacgcgatgc gcgcggccgc gcggatgaaa aagacccaga
       61 gcgtgctgat cgggctctcg gggcggggcg acaaggacgt acacacggtg gcccaggccc
      121 tcgacggcgc ggccgccaac gggcacggcg gccgccgatg aagcgcccgg ccgccgcccc
      181 gcgtccggcg tcgcggctcg ccgcgacgtt cgcggcgctc cgggcgcgtc gcgagcgtgc
      241 gctcgtcgcc tacttcacgg cgggtgatcc ctcgctcgcg atgacgcggc ggctggtggg
      301 ggaagcggcc cggcggggtg ccgacgtcat cgagctgggc atcccgttct cggatccgct
      361 cgcggacggg ccggtgatcc agcgggccag ccagcgcgcg ctcgctgcgg gggtgaccct
      421 gccgcgcgtg ctcgagctgg tgcgcgagat gcggggcgag gtgtcggtgc cgctcgtgtt
      481 cctgacctac tacaacccga tcctcgcctt cgggctcaag gccttctgcc gcaccgcggt
      541 ggaggcgggt atcgacggcg tgatcgtggc cgacgtgccg atcgaggaag cggctccgct
      601 gcgcgcggag gccgagccgg ccgggctcga cgtcgtgcac ctggtcgcgc ccacctccac
      661 cccggagcgg atgcgcaaga tcgcccgggc cagccagggc ttcatctaca tggtctcgct
      721 caccggggtc accggggagc gcagcacgct cgcccccgag ctcgcccagc agctccgtgc
      781 cctgcgcaac atcacgacca cgccgatctg cgtgggcttc ggcatcagca cgccggagca
      841 ggcggccgag gtcggccgcg ccgccgacgg tgtgatcgtg ggctccgcga tcgtgcgcct
      901 cgtcgagcag tacgggacct ccgccgagct cctcgcgcgc gtgggcgact tcatcgcgtc
      961 gctgaaggcg cccctgaaag gagaacggtg ataagccgat acggttgtcc gcccacagcc
     1021 ggctgtccgc cgagctcggg ctgtccgctg tctgggggag tgcgggggcc atttctgggc
     1081 ccccgcatta gacagagcag cggatgcgat cgcagcaagg ctcccgggtt gacagactga
     1141 tatgtccgcc tggttctgga aaaagaaaga cggcgacggt gacgagcgcc cgaagaaggt
     1201 cgtcatcgcc gagggcctgt gggtcaagtg cgactcctgc aaggagatcg tctaccgggc
     1261 cgaggtggag cgcgcgggcc gcgtctgccc caagtgcggc tacccgttcc gcatctccgc
     1321 gcgcgagcgc atcgcgctgg tggccgacga cggctccttc gaggagcgcg aggcggggct
     1381 gcgctcgaag gatccgctca acttcaagga caccaagaag tacaccgacc ggctgaaggc
     1441 cgcccgcagc aagaccgacg cgggcgaggc ggtggtcacc ggcaccgcgc gcatcggcgg
     1501 ctacccggtc gcgatcgctg tcttcgagtt cgcgttcctg ggcggcagca tggcctcggt
     1561 ggtcggcgag aagctcaccc gcaccatcga gctggccctg cagaagcgcc tcccgctcct
     1621 catcatctcc gcctcgggcg gggcgcgcat gcaggagggc atcctctcgc tgatgcagat
     1681 ggccaagacg tcggccgcgc tccagcgcct cgacgccgag cggatcccct acatctcgct
     1741 cctcaccgat cccaccaccg gtggcgtgac ggccagcttc gcgatgcttg gcgatctcat
     1801 cctggccgag ccgcgcgcgc tcatcggctt cgcggggccg cgggtgatcg cggagaccat
     1861 ccgccagccg ctgcccgacg ggttccagcg ctcggagttc ttgctcgagc acggccagct
     1921 cgacctggtc gtggagcggc gggagctgcg ggccacgctc cgacgcatcc tcgccttctt
     1981 cagcggtcgc gcccccacgc ccaccccatg acgtatgcgg aggccgtggc ccgcatcctc
     2041 gcgctgcgcg gcggggagcg gacgggcatg cgccccggcc tcgagcgcat cgaggcgatg
     2101 ctcgacgcga tcgggcggcc cgagcaggcc ttcaccatcg cccaggtggg cggcaccaac
     2161 ggcaagggct cgatctcggc catgctcgcc gcgattctcc aggccgcggg ccgccgcgtc
     2221 ggcctctaca cctcgccgca cctcgcccac tatcgcgagc gcatccgggt gaacggccgg
     2281 ccgatctccg attccgattt cgtggacggc gtggacgcgc tcggcacgct catcacccgc
     2341 ctcgacctca ccgtcttcga ggcgggcacc gcgctggcgc tcgaccactt ctgccgcgag
     2401 cgggtggacg tggcagtcct ggaggtgggc ctcgggggcc ggctcgatgc caccacggtg
     2461 ggcaagccgc gggtggtcgt cctcgggtcc atcgactacg accaccagca cgagctgggc
     2521 gatacgctgg cgttgatcgc gaccgagaaa gccgcgatca ttcgcagcgg cgtggccttc
     2581 tcggcacggc aggatccgga ggtggaggcg gtcatcatcc ggcgcgccgc cgaagccggg
     2641 gtgccgctgt cgctcgaggg gcgcgacctg cgtgtcaccc cgctcggttt caccctcgag
     2701 gcgcagcggc tcgatctgga ggggccggac tggcgaatcc ccgacgtgtc ctgcgggctg
     2761 ctgggcgtgt accagccggg caacgccttg ctggccgcgg cggcggcgcg ggagctgggg
     2821 gcggacgagg gcgcgatccg cgcgggcctg cgcggggccc gctggccggg gcggttccag
     2881 atcttccgtc gcgacccgct cgtcatcctc gacggggccc acaacccggc gggggcccgc
     2941 gccctcgcgg cgtcgctgcg cgcgtatttc ccgggccggc ccgtcacctt cgtgatcggc
     3001 gtcctggccg acaaggacgc gggcggcatc ctggccgccc tccggccgct ggccgcccgc
     3061 atcatcctca ccgccgccgc gaatccgcgg gcggccccgc ccgacgccct gcaggccctg
     3121 ctgcccgcgg gtacgcgcgt ggccaccgcg cggtcaccgc aggccgcgct cgccatggcc
     3181 actgcggagg acccgcacgg gatcgtctgc atcgccggct ccctttccct gatcggcgac
     3241 gtcctggccg tcgcaggcga cgggcaggat gtgcttttca cggccggagc ccgctgctag
     3301 catggggcct catgtcggtc ccccgcggtc ccgtccgctc gatgtccggc cctagcgggc
     3361 tcgcgcgcgg cacccggatg tttgtgttcg tgggcctgct cacggctctg ctcgcctgcg
     3421 tggagctctc cgcaggcccg gcctgggcgc agccgggcgg cacgacggtc cgtgcgggtg
     3481 gcgaggaagc caccgtcatc gccgaccagc tccagcaggt gggcggcgcc accgatctgc
     3541 tcatcgcgat cggcaacgtg gagatcatcc ggggccagac ccgcctgctc gccgaccgcg
     3601 tggagctcaa tcgcgacacc ggccaggcgg tggcccaggg gaaggtggtc ttctacgacg
     3661 ggccggaccg cctggtgggc gagcgcatcg actacaacct caagaccggg accggggtgg
     3721 tctaccaggg ctccgcggtc tcggccccgt tctatcacct ctcggccgag cagatggacc
     3781 gcgtcggcga cagcgtctac gagatgcggc gcggtgtgtt caccacctgc gagggcgacg
     3841 acccgccctg gtcgttccgg ttcggctcgg gcaccgccga tctcgacgac atcgtttacg
     3901 gacgcgacgc ctcgttctgg gtgaatggcg tcccggtgct gccgtgggtg ccgttcttcg
     3961 cggcggccct gcggcgcgag cgccagtcgg gtttcctctt cccggagttc ggccagtcct
     4021 cgcgcaaggg cgtcttctcg aagatcccgt acttctgggc gatcgacgac agccaggatc
     4081 tcacggtcag cttggacgct tacagccggc gcggcgtcgg gctcgagggc gactaccggt
     4141 acgtgatgtc ccgtgagcag cgcggcgagc tgaccctctt cggcgtcgac gagttcctcc
     4201 gcgacgatcc acggctgcag gagaatcgcg gctgggcgtc ctttcgccac gactggcaga
     4261 tcaccccacg gttgtccttc aaggtcaacg ccaacgtcac cagcgacgac ctcatctttc
     4321 gcgactacgg caaccggctg gcggagcgcg ccacccagcg ggccgagacg aacgtgttcg
     4381 tcacccagaa ctgggacgcc tggagcctgg tcggcaacgt gaagtggtac caggatctca
     4441 ccaccccgcg gccgatcgag ctgcagcggg tgcccgagat caaggtggtg ggcatccggc
     4501 agccggtgcc cggggtgccc gggctgctct acgagaccac cgcgagcttc gtgaacttca
     4561 tccgcgacgt ggggcccgac gggatccggg ccgacttcca cccgcggctt ctgatgccgc
     4621 ttccggtcgg cggcgtgttc acggtcacgc cgttcgcggg cggccggctg acctactacg
     4681 acgagcgtgt ggtagaccag cggctcaccc gcgtgggtgc cctctcggtg gagaagtcgg
     4741 tgcacgatcc gcgcgtccgc cgccaggtgg agtggggggc ggaggcggag agccgcgtct
     4801 cccgcgtcta taccagcgcc ggcgggagcg gggtcgcggc gtaccagcac gtgatcgagc
     4861 cgcgagtgcg gatcatccag atccgtggcg tcgagcagaa ggactttccc cagtacgatc
     4921 ccggcgcgag caccgccacc gggatcgatc ccggctacga gcggcgcgtt ggcatcgacc
     4981 gggtcggcaa ggccaacgag atcacctact acctcaccaa ctcgctgaac gccaagacgg
     5041 tggccgggcc cgaccagttg ccggtgcgat gggagatggt acgcttcacc ttgagccaga
     5101 cgttgaagat ggacccggtc tcccagccct tgaaggatct ctacggcgat ctcacggtca
     5161 gccccaacca aaggatcggc ttccacgccg atgcccggta caatgtctat gatctgggcc
     5221 tgcgagaggc caac
//
LOCUS       DASUFA010000444         6532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_678706, whole genome shotgun sequence.
ACCESSION   DASUFA010000444 DASUFA010000000
VERSION     DASUFA010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678706"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VFX87_16135"
     CDS             <1..1977
                     /locus_tag="VFX87_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX4996510.1"
                     /translation="RSVVQWDKDDCAALGIVKVDLLGLGMMSLLQDALEMIRRRGGAV
                     DLAHLPQNDPEVYAMLQQADTIGVFQVESRAQMATLPRLRPERFYDLVVQVAIIRPGP
                     IVGDMVHPYLRRRAGREPVTVPHPALESILKRTLGVPLFQEQLLRMAMATAGFTGGEA
                     EELRRAFGFKRSERAMREVEVKLRAGMARQGISGAAAEAIIHSITAFALYGFPECVVG
                     ETLVIDAATGHRVKIEEVVKESAPLTHTFACDADLKIRKRRVLAATASGPRMVHRLRT
                     ALGRELLATPEHPLLTVNGWQPLGSLRPGDHIAAPRTPEMADVDWDRVVNVEPVGTRE
                     TYDLTVEGDHNFVANDLIVHNSHAASFALLAYASAYLKAHHPAAFYAALLNNQPMGFY
                     DPATIVGDAGRHGQGIRPADVSFSDWLCAIEPDGVVRLGLRYVRGLREEAGRRLEAER
                     RRRPFASVEDVVKRGGLRRDELAQLARVGALASLEPERRAALWEAERAGRPAGALYED
                     VPAPRELSPLRPMSAEERLVADYEGTGLTLGPHPMAFRRGALLRMGVSRAADLAAFRH
                     GAWVKVAGAVVVRQRPGTAKGFVFLNLEDETGLVNVIVRPALFHRHRLALVNEPFLLI
                     EGTLQRQDNVTSVRAERLAPLRHRLASVPSHDFH"
     gene            complement(1901..2905)
                     /locus_tag="VFX87_16140"
     CDS             complement(1901..2905)
                     /locus_tag="VFX87_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4996511.1"
                     /translation="MDLNPAHVRTLREIARHGSFSRAAESLRLSQPAVSLHMHQLEAR
                     CGTALLERIGKRAFPTPAGAVLLEHAGRAFAELEAAQEALRQLRGVVAGRLRLGTGAT
                     ASIHLLPPLLRRIRVRYPELELIVVTGNTPEIAAAVAANELDVALVTLPVTGRQLLVT
                     PLLLDPLVAVGPPAQAWPRRGGLTAAELARHPLIVYERGGTIRRVIDGWFRRGGARPR
                     VVMELGNEEAIKKLVGAGLGVSVSPAMAVRDEVRSGALSMRPLSPALVRRLGLVRRRD
                     KPETPALRMFLAALEGLPRGSGLARCSRRRAERISENRGRARSPAGGAAARAARRAPR
                     "
     gene            2998..3492
                     /locus_tag="VFX87_16145"
     CDS             2998..3492
                     /locus_tag="VFX87_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEX4996512.1"
                     /translation="MGDVSVRPATPDDAAAICTIYNQGIEDRIATLETELRTPEERRQ
                     WMAARGPRHPVVVATTDGQVVGWGSLNSFNARPAYDNVADLSVYVERAWRGRGVGTVL
                     LQHVLGLARQIGYHKMVLATFPYNDAGLALYRRMGFTPVGVYHEQGRLDGRWVDVLIM
                     EQLL"
     gene            3549..4661
                     /locus_tag="VFX87_16150"
     CDS             3549..4661
                     /locus_tag="VFX87_16150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996513.1"
                     /translation="MLYYGFPVFLASMERDLGASRAAVTGAFTVGMGIAALAALPVGR
                     WLDRRGPWALMTVGSCLGTALLVAWSRTVSLTGFYVIWTLMGLALAATLYEPAFGAVV
                     RWFPGENRDRALTIVTLAGALASTIFMPIAAWLVEHGGWRQALLVLAAILAELTIPLH
                     AVVLRRQPPHVDRTNKRAPHGPGSTLGEASRTPIFWALAVAFSIANFSTTAVTLHLIP
                     YMVAHGWSAVTMAVSIGWMGAMQIPGRLLFVAVTARFGALGVTMTVFLAQAVGLALVA
                     LAPSLPGGLFLMIVVLGAANGMGTLARATTLAEVFGAGHYASIGGAVALGANGARAAG
                     PLGASLLYLALGGYRAVFWALTGAGGGRRGGRGRRP"
     gene            4737..>6532
                     /locus_tag="VFX87_16155"
     CDS             4737..>6532
                     /locus_tag="VFX87_16155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4996514.1"
                     /translation="MLAGWGRLVHRFRLAIIALSLLSLVPSLWLIFHGGRLATTDIPT
                     TTESGRALDLIGRELPGRPPSFSFIFSSHTRAAQDPAFRQEVERAVAPLRRDGRVARV
                     LTAYDPPVHDASVPAAQLVSRDGRRVLVVVELKGEAAAFSSLEFSVLPPDVYPSLRAL
                     VKTETLEVLPVGNIPLNHDFTEVTRTDLERAELVILPVVVVLLLLVFGSVVAALLPLV
                     VGALSMAGAIAATLLMARYTSVSVYAPNIVTMIGLGVAIDYSLFIVSRFRQEIREHPG
                     SEALERTMATAGRAILFSGLTVAIGLLGMVFLGLGNIGSIGWAGTVVVSLAVLYALTL
                     LPALLAVLGPRVNALRLPFVHPERRVTGRGIWHRIAVTVMAHPWAVFISVSVLLLFLG
                     LPFLHLKVGSGDVNALPPEAVSRRGDEVLREHFPGADSNRILVVVRYPDGTPLTAERV
                     GGLYDLSRWLAARPNVARVESLVDLDPSVTREQYQQIATAPAEMLPPGVAQVLRQTVG
                     GRLALLVVSTALKPSSTEARALVEEIRASHPRLEGEVLVTGQTAFDLDIIDLVTRHAP
                     LTVGLVMVATYVVLFLLLGSVLLPLKAVIMNLL"
BASE COUNT          799 a         2367 c         2352 g         1014 t
ORIGIN      
        1 cgcagcgtgg tgcagtggga caaggacgac tgcgccgcgc tcggcatcgt caaggtggat
       61 ctcctggggc tcggcatgat gtcgctgctc caggacgcgc tcgagatgat ccggcgccgc
      121 ggcggcgcgg tggacctggc ccatctcccc cagaacgatc ccgaagtcta cgccatgctc
      181 cagcaggccg acaccatcgg cgtcttccag gtggaaagcc gcgcccagat ggcgacgctg
      241 ccgcgcctgc gccccgagcg cttctacgat ctggtggtgc aggtggccat catccgcccc
      301 gggcccatcg tgggcgacat ggtgcacccc tacctgcggc gccgcgcggg ccgcgagccg
      361 gtcacggtgc cgcatcccgc gctcgagtcg atcctgaagc ggacgctcgg cgtgccgctc
      421 ttccaggagc agctgctccg catggcgatg gccaccgccg gcttcaccgg cggcgaggcc
      481 gaggagctgc gccgcgcctt cggcttcaag cgctcggagc gggccatgcg ggaggtggag
      541 gtgaagctgc gcgcgggcat ggcgcgccag ggcatctcgg gcgccgccgc cgaggccatc
      601 atccactcca tcaccgcctt cgccctgtac ggcttcccgg agtgcgtggt gggcgagacc
      661 ctcgtcatcg acgcggcaac cggacaccgg gtgaagatcg aggaggtggt gaaggagagc
      721 gcgcccctca cgcacacctt cgcgtgcgat gcggatctca agatccggaa gcgccgggtg
      781 ctcgcggcca ccgcgagcgg ccctcggatg gtccatcgcc tccgtaccgc cctgggccgg
      841 gagctgctgg ccaccccgga gcaccccttg cttaccgtca acggctggca gccgctcggg
      901 agcttgcgtc ccggcgacca catcgcggcg ccccggacgc cggaaatggc cgacgtcgat
      961 tgggatcggg tggtgaacgt ggagccggtc gggacgcgtg agacgtatga cttgaccgta
     1021 gagggtgacc acaacttcgt ggccaacgat ctgatcgttc acaactccca cgccgcgagc
     1081 ttcgcgctgc tggcgtacgc gagcgcgtac ctgaaagcgc accacccggc cgcgttctac
     1141 gcggcgctgc tcaacaacca gccgatgggc ttctacgatc cggccaccat cgtcggcgac
     1201 gcgggccggc acgggcaggg gatccggccg gccgacgtga gcttctcgga ctggctctgc
     1261 gcgatcgaac ccgacggcgt cgtgcggctg gggctgcgct acgtgcgcgg gctgcgggaa
     1321 gaggcggggc ggcggctcga ggcggagcgc cggcggcggc ccttcgcctc ggtcgaggac
     1381 gtggtgaagc gcggcgggct ccgccgcgac gagctggccc agctcgcgcg ggtgggcgcg
     1441 ctcgcgagcc tcgagccgga gcggcgggcc gcgctgtggg aggcggagcg cgcggggcgt
     1501 cccgcggggg cgctctacga ggatgtgccc gccccgcgag agctctcgcc gctacggccg
     1561 atgagcgccg aggagcggct ggtcgccgac tacgagggca ccggcctcac cctcgggccg
     1621 caccccatgg ccttccggcg cggggcgctc ctgcggatgg gcgtgtcgcg cgcggcggat
     1681 ctggccgcct tccgtcacgg ggcgtgggtg aaggtggcgg gagcggtggt ggtgcgccag
     1741 cggcccggca ccgccaaggg cttcgtcttc ctgaacctcg aggacgagac gggcctggtc
     1801 aacgtcatcg tgcggccggc gctcttccat cgccaccggc tggccctggt caacgagccc
     1861 ttcctcttga tcgagggcac cctgcagcgc caggacaacg tcacctcggt gcgcgccgag
     1921 cggctcgcgc cgctgcgcca ccggctggcg agcgtgccct cccacgattt tcactgatcc
     1981 gctcggctcg ccttcggctg cacctcgcca agccactgcc cctgggcaac ccttcgaggg
     2041 ccgcgaggaa catgcggagc gccggcgttt cgggcttgtc gcgccggcgc acgaggccca
     2101 ggcgccgcac cagggcgggc gagagcggcc gcatggaaag cgcgccggag cgcacctcgt
     2161 cccgcaccgc catcgccggg ctcaccgaga cgccgagccc ggctcccacg agcttcttga
     2221 tggcctcctc gttgccgagc tccatgacca cccgcggccg cgcgccgcca cgccggaacc
     2281 agccgtcgat cacgcgccgg atcgtcccgc cccgctcgta gacgatgagc gggtggcggg
     2341 ccagctcggc cgcggtgagc ccgccgcgcc ggggccatgc ctgcgcgggc gggcccaccg
     2401 ccaccagcgg gtcgagcagg agtggcgtca ccaggagctg acggccggtc accggcaggg
     2461 tcacgagggc cacgtcgagc tcgttggccg cgacggcggc cgcgatctcg ggcgtgttgc
     2521 cggtcacgac gatcagctcg agctcggggt agcgcacccg tatccgtcgc aacagcggcg
     2581 gcagcaggtg gatgctggcg gtggcgccgg tgccgaggcg cagccgccct gccaccaccc
     2641 cgcggagctg gcggagggct tcttgggcgg cctcgagctc ggcgaacgcg cgcccggcgt
     2701 gctcgagcag caccgccccc gccggggtgg ggaaggcgcg cttgccgatc cgctcgagca
     2761 gcgcggtgcc gcagcgggcc tcgagctggt gcatgtgcag gctcaccgcg ggctggctca
     2821 ggcgcagact ctccgccgcg cgcgagaagc tgccgtggcg cgcgatctcc cggagggtgc
     2881 gcacgtgggc gggattgaga tccacgattc agaagtattt ctcatcgttc gtatgaggac
     2941 aatctatttc ccttatcgtg gccgcgcggg cagactgcgg ggtaaggaga aaccctcatg
     3001 ggggacgtct cggtacgccc cgcgacgccg gacgacgccg cggccatctg cacgatctac
     3061 aaccagggca tcgaggaccg catcgccacg ctcgagaccg agctgcgcac cccggaggag
     3121 cgccggcagt ggatggccgc gcgcggcccc cgccacccgg tggtggtggc gaccaccgac
     3181 ggccaggtgg tcggctgggg cagcctcaac tcgttcaacg cgcgcccggc ctacgacaac
     3241 gtggcggatc tctcggtcta cgtcgagcgc gcctggcgag gccgcggggt gggaaccgtg
     3301 ctcctgcagc atgtgctggg cctcgcgcgc cagatcgggt accacaagat ggtgctggcc
     3361 acctttccct acaacgacgc cggcctcgcg ctctaccggc gcatgggctt cactccggtc
     3421 ggcgtctacc acgagcaggg ccggctcgac ggccgctggg tggacgtcct gatcatggag
     3481 cagctcctct gagctacggc tggcgcgtcg tcgcgacgct ctccgtcacc gaggtcgtct
     3541 cctggggcat cctctactac gggttccccg tcttcctcgc ctcgatggag cgcgatctgg
     3601 gcgcctcccg ggctgcggtc accggcgctt tcacggtcgg catgggcatc gcggcgctcg
     3661 cggccctgcc ggtgggccgc tggctcgatc ggcgcggccc gtgggccctc atgacggtgg
     3721 gctcgtgtct cggcaccgcc ctgctggtcg cctggtcgcg cacggtgagt ctcaccgggt
     3781 tctacgtgat ctggacgctg atggggctcg ccctggccgc cacgctctac gagcccgcgt
     3841 tcggggcggt ggtgcgctgg ttcccgggcg agaaccgcga ccgcgcgctc accatcgtca
     3901 cgctcgcggg ggcgctcgcc agcaccatct tcatgccgat cgcggcctgg ctggtggagc
     3961 atggcggctg gcggcaggcg ctgctcgtgc tggccgcgat tctcgcggag ctgacgattc
     4021 cgctgcacgc cgtcgtgctg cgccgccagc cgccgcacgt cgatcggacg aacaagcggg
     4081 cgccgcacgg acccgggtcc accctcggcg aggccagccg caccccgatc ttctgggcgc
     4141 tggcggtggc gttctcgatc gccaacttct cgacgaccgc ggtcacgctg cacctgatcc
     4201 cgtacatggt cgcgcacggc tggtccgcgg tgaccatggc cgtctcgatc ggctggatgg
     4261 gcgccatgca gatcccgggg cgcctgctct tcgtggcggt gaccgcgcgc ttcggcgcgc
     4321 tcggggtgac gatgacggtg ttcctcgcgc aggcggtggg gctcgcgctc gtcgcgctcg
     4381 cgccgtcgct gccgggcggc ctgttcctga tgatcgtcgt gctcggcgcc gccaacggca
     4441 tgggcacgct cgcgcgcgcc accacgcttg ccgaggtgtt cggcgccggg cactacgcga
     4501 gcatcggcgg cgcggtggct ctgggcgcca acggcgcgcg cgcggccggg ccgctcggcg
     4561 cctcgctgct gtatctggcg ctggggggct atcgtgcggt gttctgggcc ctgacgggcg
     4621 ctggtggtgg tcggcgtggt ggtcgcggtc gccgcccgtg aggcgtggga gcccgccgcc
     4681 gcgcccggag cgtaaaccgc cggaccgatc gtcggttcgc ggtacgatag gtcgtcatgc
     4741 tcgcgggatg gggtcgcctc gttcatcgct tccggctggc gatcatcgcc ctgtccctgc
     4801 tgagcctggt cccgtcgctg tggctgatct tccacggcgg ccgcctcgcc accaccgaca
     4861 tcccgacgac cacggagtcc ggccgcgccc tcgatctgat cgggcgcgag ctgcccggcc
     4921 ggccgccctc attcagcttc atcttcagca gccacacgcg cgcggcccag gaccccgcgt
     4981 tccgccagga ggtggagcgg gcggtggcgc cgctccgccg cgacgggcgc gtggcccgcg
     5041 tgctgaccgc gtacgacccg ccggtccacg acgcctcggt gccggccgcg cagctcgtct
     5101 cgcgcgacgg ccgacgggtg ctggtggtgg tggagctgaa gggcgaagcc gccgcgttct
     5161 cctcgctcga gttctcggtg ctccccccgg acgtgtaccc ctcgctgcgg gccctggtga
     5221 agaccgagac cctcgaggtg ctgccggtcg ggaacattcc gctcaaccac gacttcaccg
     5281 aggtgacgcg caccgacctc gaacgggccg agctggtgat cctgcccgtc gtggtggtgc
     5341 tgctcctgct ggtattcggc tcggtggtgg ccgcgctcct gccgctggtg gtgggcgcgc
     5401 tctcgatggc gggcgccatc gcggccaccc tgctcatggc ccgctacacc tcggtctcgg
     5461 tctacgcccc gaacatcgtg accatgatcg gtctcggggt ggccatcgac tactcgctct
     5521 tcatcgtgag ccgcttccgc caggagatcc gcgagcaccc cggatcggag gcgctcgagc
     5581 gcacgatggc caccgccggg cgggcgattc tcttctccgg gctcaccgtc gccatcgggc
     5641 tgctcggcat ggtcttcctc gggctcggca acatcggctc gatcggctgg gccggcaccg
     5701 tggtcgtctc gctggccgtg ctctacgcgc tgacgctcct gcccgcgctg ctggccgtgc
     5761 tcgggccgcg cgtcaacgcg ctccggctgc ccttcgtcca tcccgagcgt cgcgtcaccg
     5821 gccgcgggat ctggcaccgg atcgcggtca ccgtgatggc ccatccgtgg gcggtcttca
     5881 tctcggtctc ggtcctgctg ctgttcctcg ggctgccgtt cctgcacctg aaggtgggct
     5941 cgggcgatgt caacgcgctg ccgcccgagg cggtctcgcg tcgcggcgac gaggtgctgc
     6001 gcgagcactt cccgggcgcc gacagcaacc gcatcctcgt ggtcgtgcgc tacccggacg
     6061 gcactcccct gaccgccgag cgagtcggcg gcctctacga tctcagccgc tggctcgccg
     6121 cgcggcccaa cgtggcgcgg gtggagagtc tggtggacct cgatccgagc gtcacgcgcg
     6181 agcagtacca gcagatcgcc accgcgcccg cggagatgct accgcccggc gtggcccagg
     6241 tgctgcggca gacggtcggg ggccgcctgg ccctgctggt ggtctcgacc gcgctcaagc
     6301 cgtcgagcac ggaggcgcgc gccctggtgg aggagatccg cgcgtcgcac ccgcggctcg
     6361 agggcgaggt gctcgtgacg ggccagaccg ccttcgatct cgacatcatc gacctggtca
     6421 cacgccatgc cccgctcacc gtcggcctcg tgatggtcgc gacctacgtg gtgctgttcc
     6481 tgctgctggg ctcggtgctg ctgccgctca aggcggtgat catgaacctc ct
//
LOCUS       DASUFA010000445         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_678817, whole genome shotgun sequence.
ACCESSION   DASUFA010000445 DASUFA010000000
VERSION     DASUFA010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678817"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFX87_16160"
     CDS             <1..393
                     /locus_tag="VFX87_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4996515.1"
                     /translation="EPGIERKGRILLVDNDPQVLSILGEMLKDAGHHVLPVPSGSEAL
                     RVFVPSGFDLVITNVGMPEMSGWDVAEQLRARDPNVPVIFITGWGLQEEDQARCRRLG
                     ISALLFKPVPPPELHRKVQVALASARPR"
     gene            complement(506..1876)
                     /locus_tag="VFX87_16165"
     CDS             complement(506..1876)
                     /locus_tag="VFX87_16165"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX4996516.1"
                     /translation="MPTRPPDLPTTLQPTPERTAALDRLGDQIAELSAHLDAATARLL
                     TLIREFDARGGWSNGFHSCAEWLAWRVGLAPGAARERVRVARALGTLPALAEALARGE
                     LSYAKVRALTRIATPETEARLLAVGRAGTAVHVERIVRGWRQVDRQAEARKAARQHAG
                     RALHVYQDTDGTVVVRGRLTPEGGALLLRALDAARETLYQRRRATEAVGLATDPAVDA
                     PTRPQQHADALALLAETALHHELDPGTPGERYQVVVHVDAAVLADAEWPGQSMLEDGP
                     HVPAGTSQRLACDASRVVMRHDADERVVEVGARTRTIPPALRRALQHRDKTCRFPGCH
                     VRVAEGHHVRHWAQGGPTTLSNLALLCRRHHRAVHEEGYQLERLSDSTLQFRRPDGRV
                     LPDVPPPAAVPADPAGAIRAQNAAQGLNLHARTGLAQWFGERLDVGWAIDVLHPRATR
                     PGSGLV"
     gene            complement(2187..3332)
                     /locus_tag="VFX87_16170"
     CDS             complement(2187..3332)
                     /locus_tag="VFX87_16170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX4996517.1"
                     /translation="MQNAVIVGAVRTAVGRKNGKLSGYRPDDLLADTLKALVARVKID
                     PSEVEDVVMGCVDQVGEQGLNIARNAALIAGFPLDVCGTTLDRMCGSGQQAANFAAMG
                     VMSGQYECVIAGGVENMSRVPMGSNGMGPGDGPLSPRIQALYNIIPQGLSAEMVAEQW
                     GIKREELDEFSAQSHERAGRAIAEGRFKREIAPLTLADGSVFDTDEGVRVPVNREKMA
                     ALAPSFKPDGVITAANSSQISDGAAALLIMSEARAKALGLKPRARIVATALAGVDPTI
                     MLTGPIPATQRVLKKAGLKLDQIDLFEINEAFASVVLAWERELHPDMSRVNVNGGAIA
                     LGHPLGCSGGKLMTTLLHELERTKKRYGLQTMCIGFGQGIATIIERL"
     gene            complement(3355..4263)
                     /locus_tag="VFX87_16175"
     CDS             complement(3355..4263)
                     /locus_tag="VFX87_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4996518.1"
                     /translation="MKCRKCGGAAALELRRHNAAFCSPHFLEFFRKQVAEAIHRHHMF
                     TRDEKIMVAVSGGKDSLALWDVLIEDGYQTAGLYLDLGIFDYSKESKAKCEAFAASRG
                     QTLHIERVAEAVGAPIPVVQQATRRPTCSACGLSKRYLMNRAALQHGYPVVATGHNLD
                     DEAATLFGSVMHWQTDALPRQSPALASTHPKLVRRVKPLYRLSELETAAFAFLRGIDY
                     IVEECPFAKGATSIAHKEVLNRLEEASPGSKHNFLFGFLEKARPAFEKIENVELLECT
                     SCGQVTTGAICAFCKLADLVKRRTST"
     gene            complement(4260..>4528)
                     /locus_tag="VFX87_16180"
     CDS             complement(4260..>4528)
                     /locus_tag="VFX87_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996519.1"
                     /translation="GRSPAAAPRGEGVRGVPRIVKVILRNPRKEIVVDGRRRVKELLV
                     ELEILPSTVLVIRGNELLTGDTVVGGDDVLEVRPVMSGGSTGTP"
BASE COUNT          639 a         1606 c         1586 g          697 t
ORIGIN      
        1 gagccgggca tcgagcggaa ggggcgcatc ctgctcgtgg acaacgatcc gcaggtgctc
       61 tcgatcctgg gcgagatgct caaggacgcg ggccaccacg tgctgcccgt cccgagcggg
      121 tcggaggccc tccgggtctt cgtccccagc ggcttcgacc tcgtcatcac caacgtgggc
      181 atgccggaga tgagcggctg ggacgtggcc gagcagctcc gggcccgcga cccgaacgtc
      241 ccggtgatct tcatcaccgg ctggggcctg caggaggaag atcaggcgcg ctgccgccgg
      301 ctcggcatct ccgcgctgct cttcaagccg gttccgccgc ccgagctaca ccgcaaggtg
      361 caggtggcgc tggcctccgc ccgcccgcgc tagccggaat ggtcgcggct acggccgcgc
      421 cttcattgac cgcaggggaa gcccgagagt tgtacgctcc tcgttgattg ccgcctcgta
      481 gtttctgcgc tgctcaggta gccgactaca ccagtcctga accgggtcgc gttgcccgcg
      541 gatgcagcac gtcgatcgcc cagcccacgt ccagccgctc cccaaaccac tgggcgagcc
      601 ccgttcgcgc atgaagattg agtccttgcg cggcgttctg tgcccggatc gctccggccg
      661 gatcggcagg taccgccgcc ggcggtggca cgtcaggcaa tacgcggcca tccggccgcc
      721 ggaactggag cgtgctatcg gacaaccgct cgagctggta gccctcctcg tgcaccgcgc
      781 ggtgatggcg gcggcagaga agcgcgaggt tggacagcgt tgtcgggccc ccctgtgccc
      841 agtggcgcac gtgatgccct tcggcgaccc ggacatggca gcccgggaag cggcaggtct
      901 tgtcgcgatg ctgcagggcc cggcgtaggg ccggcggaat cgtgcgcgtc cgggcgccga
      961 cctctaccac ccgctcgtcc gcgtcatgcc gcatcaccac ccggctcgcg tcgcacgcca
     1021 gccgctgcga cgttcccgcg ggaacgtgtg ggccgtcttc cagcatcgac tgcccgggcc
     1081 actccgcgtc cgccaggacc gcggcgtcca cgtggaccac cacctggtac cgctcccccg
     1141 gggtccccgg gtcgagttcg tgatgcagtg ccgtctccgc cagcagcgcc aacgcgtcgg
     1201 cgtgctgctg cggccgggtc ggcgcgtcca cggccggatc agtggccagc ccgacggctt
     1261 cagtggcgcg gcgccgctgg tacagcgtct cgcgggcggc gtcgagggct cggagaagca
     1321 gggcgccccc ttccggcgtt agcctgccac gcaccaccac cgtgccgtcc gtatcctgat
     1381 agacgtgcag cgcacggccc gcgtgctggc gcgcggcctt gcgagcttcg gcctgccggt
     1441 ccacctggcg ccagcctcgt acgatgcgct ccacgtggac cgccgtgccc gcgcgcccca
     1501 cggccagcag gcgcgcctca gtctccgggg tcgcgatgcg ggtcagggcg cggaccttgg
     1561 cgtaggacag ctccccgcgc gccagcgctt cggccagggc cggcagtgtc ccgagggcac
     1621 gggccacccg gacgcgctcg cgggcggcgc ccggcgcgag tccgaccctc caggcgagcc
     1681 actctgcgca ggagtggaaa ccgttactcc agcctccgcg ggcgtcgaac tcgcggatga
     1741 gcgtgagcag tcgtgccgtc gccgcgtcga ggtgggcgga gagctcggcg atctggtcgc
     1801 ccaggcgatc cagcgccgcg gtgcgctccg gcgtgggctg caacgtggtg ggaaggtccg
     1861 gcgggcgcgt cggcatgggg agtcctccct tctggtggga cgactctaac cccggggttc
     1921 gggggcgcct gagaccgcgc aggtcgcgcg aacgggcggc acaccatcat cgttcgtgcc
     1981 tcgcggcgcg accacgcacc acacgccgga tgcgaagggc aggattggca tttcctacac
     2041 gcactcgaca cacgacgagg cgcccgtgaa aatccgtcaa gcggaaaagt gcggaggccg
     2101 ccgcgtcgct gggagcgccc gcgcgcgacc acccctcacc ctgccctctc ccctgagggg
     2161 agaggggtat agggtgggga cgactcttac aggcgctcga tgatggtggc gatcccctgc
     2221 ccgaagccga tgcacatcgt ctggagcccg tagcgcttct tggtgcgctc cagctcgtgc
     2281 aggagcgtgg tcatcagctt gccgcccgag cagccgagcg ggtggccgag ggcgatcgcg
     2341 ccgccgttga cgttcacgcg gctcatgtcc gggtgcagct cgcgctccca ggccaggacc
     2401 accgaggcga aggcctcgtt gatctcgaac aggtcgatct ggtcgagctt cagccccgcc
     2461 ttcttgagca cccgctgggt ggccgggatg gggccggtga gcatgatggt cggatccacg
     2521 ccggccagcg ccgtggccac gatgcgcgcg cgcggcttca ggccgagggc cttggcccgc
     2581 gcctccgaca tgatcagcag cgcggcggcc ccgtcggaga tctgcgagga gttggccgcg
     2641 gtgatgaccc cgtccggctt gaacgacggc gcgagcgccg ccatcttctc ccggttcacg
     2701 ggcacccgca cgccttcatc ggtgtcgaac acgctgccgt cggccagcgt gagcggcgcg
     2761 atctcccgct tgaagcgccc ctccgcgatc gcccgaccgg cgcgctcgtg gctctgggcc
     2821 gagaactcgt cgagctcctc gcgcttgatg ccccactgct cggccaccat ctcggcggag
     2881 agcccctgcg ggatgatgtt gtagagcgcc tggatccgcg gggagagcgg cccgtcgccg
     2941 gggcccatgc cgttcgagcc catcggcacg cggctcatgt tctccacgcc gcccgcgatc
     3001 acgcactcgt actggccgga catcacgccc atggccgcga agttggcggc ctgctggccg
     3061 gagccgcaca tgcggtcgag cgtggtgccg cagacgtcca ggggaaagcc ggcgatcagc
     3121 gccgcgttgc gggcgatgtt gagcccctgc tcgcccacct ggtccacgca gcccatcacc
     3181 acgtcctcga cctcgctcgg gtcgatcttc acgcgcgcca ccagggcctt gagcgtgtcg
     3241 gccagcaggt cgtccgggcg atagcccgag agcttgccgt tcttccggcc gacggcggta
     3301 cggaccgcgc ccacgatcac cgcgttctgc atggctgtct cctcctcgaa gggttcatgt
     3361 tgacgtgcgg cgcttgacca gatcggcgag cttgcagaag gcgcagatcg cgccggtggt
     3421 gacctgccca caagaggtgc actcgaggag ctcgacattc tcgatcttct cgaaggccgg
     3481 gcgggccttc tcgaggaacc cgaacaggaa gttgtgcttg gagccgggtg aagcctcctc
     3541 gagacggttc agcacttcct tgtgggcgat ggaggtggcg cccttggcga acgggcactc
     3601 ctccacgatg tagtcgatgc cgcgcaggaa cgcgaaggcg gccgtctcga gctccgacag
     3661 gcggtagagc ggcttcacgc gacggacgag cttcggatgg gtggaggcca gcgccgggga
     3721 ttgccgcggc agcgcgtcgg tctgccagtg catgaccgag ccgaagagcg tggcggcctc
     3781 gtcgtcgagg ttgtggccgg tggccaccac cgggtagccg tgctggagcg cggcgcggtt
     3841 catcaggtag cgcttggaga ggccgcaggc cgagcaggtc gggcgccgcg tggcctgctg
     3901 caccaccgga atcggcgcgc ccaccgcctc cgccacccgc tcgatgtgca gcgtttggcc
     3961 gcgggaggcc gcgaacgcct cgcacttggc cttggactcc ttcgagtagt cgaagatgcc
     4021 caggtcgaga tagaggccgg cggtctggta gccgtcctcg atcagcacgt cccagagcgc
     4081 gagggaatcc ttgccgcccg agacggccac catgatcttc tcgtcgcggg tgaacatgtg
     4141 atgtcggtgg atcgcctccg cgacctgctt gcggaagaac tcgaggaagt gtggcgagca
     4201 gaaggccgcg ttgtggcgcc gcagctccag cgcggccgcc ccgccgcact tgcggcactt
     4261 cacggcgtcc ccgtgctgcc gcccgacatg accgggcgca cctcgagcac gtcgtcgccg
     4321 cccaccacgg tgtccccggt caggagctcg ttgccgcgga tgaccaggac ggtgctgggc
     4381 aggatctcga gctcgacgag gagctccttg acccggcgcc ggccgtccac cacgatctcc
     4441 ttgcgggggt tgcgcaggat gaccttcacg atgcggggaa ccccccggac gccttcaccc
     4501 cgcggagctg ccgcaggtga tcggcctc
//
LOCUS       DASUFA010000446         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_681942, whole genome shotgun sequence.
ACCESSION   DASUFA010000446 DASUFA010000000
VERSION     DASUFA010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681942"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFX87_16185"
     CDS             complement(<1..996)
                     /locus_tag="VFX87_16185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996520.1"
                     /translation="MRFTWFNLMPWPYLPDDFREKNRSVWVDIPSTLYDPKKGHFVYH
                     QYMDQLEYAETLGFDGIGCNEHHQNGYGLMPSPNLIAAGLARRTSRAAICVIGNSIAG
                     YNPPIRVAEEFAMLDVISGGRLVAGFPVGTPMDTNFCYGQIPALTRDKYKEAHDMIMK
                     AWAEDEPFAFDGKYNQLRWVNCWPKPIQKPHPPIYIPGGGSIETWDFCLDYDYNYSYL
                     SFYGYLRGKSLLEGYWDRVAKRGKDDSPYRAAFAQIICVADTDAEAEELYAEHCLYFF
                     NRCLHVFPPFADPPGYRTVNTIKYGALSQLRLEAQKLMANLTWKQLVDERFIIAGS"
     gene            1053..2015
                     /locus_tag="VFX87_16190"
     CDS             1053..2015
                     /locus_tag="VFX87_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX4996521.1"
                     /translation="MHGAQGLDARLGRQLVLDELFDLTLYRSLQRVAPAGLRPILEQL
                     IPIETRHHAFWQEFFGLKIATLDLPRRMKLALLVGLARLLGAPGIHLILEAIEVYGVR
                     KYLAVWRQYEGGPLGEAVRSILEDEFKHEDAVVMAESERQMNPQRVRDIFLGLNDGLV
                     EIVGAVSGFFGAFADATTVLVAGMMVGVAGALSMGAGAYLAGSSEAEVRDTEVRRRRF
                     LGEADPTEGRADTPLASGLRVGVSYVIGAVVPVLPVLFGARTLLPSLVVAGAVIVIVS
                     AILAFLSGMNVKRRILMNLAIIAAAVAISYLIGRVAQSAWGISV"
     gene            complement(2022..3275)
                     /locus_tag="VFX87_16195"
     CDS             complement(2022..3275)
                     /locus_tag="VFX87_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4996522.1"
                     /translation="MILAAFRVRSFRFQWPADLFTSWAFEMETLILGWYVMVQTGSVL
                     LLTLFGSAQFLGTLAAPMFGVLGDRVGGRLMLCAMRAIYATFAVTVMALAFTGWLTPA
                     WAIALAALNGIVRPNDLVMRNALIGETIPPGHLMGALGMSRATMDSARVGGALAGAGL
                     STMLGIGLTYVFVTCFYLASLALTFGVARARQVPDFAAPSPYPLPLSGQRDWGWRELQ
                     DGVAHILSRPQLLAPMWLAFLINLTAYPASGGLLPYIAQRVYQVDATGLGWLAAAFSF
                     GALLASIAMVVTGGPAHPERYTLVHTVLWYGLLLVFAHVPSLGGGLLVLLATGFVQSV
                     AMISMTATLLTASGPGFRARVMGVRSLCVYGLPIGLIASGALIERIGYPLTLTVLAAL
                     GLVFTALIAVRWRAAMWGRARALTT"
     gene            complement(3272..4285)
                     /locus_tag="VFX87_16200"
     CDS             complement(3272..4285)
                     /locus_tag="VFX87_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4996523.1"
                     /translation="MTTATARFGIVFLPESLTDFGELCPEAEAAGFDWLGVADSQSVF
                     RELYVALTLAALNTRRVKLGPLVTNPQTRHLVVTASAIASVDELSGGRAVLGLGSGDS
                     AIYTIGAPPATLAGLEDSIVTLGRLTAGEPVDRGGRAWRVHRSTRRVPIYLAAEGPRT
                     LELAGRVADGVICGLGLTPEVIALSLAAIERGARAAGRALEDVDVWWFAKTSLAGSRA
                     AAIEPIKMALAASANHAFRFTLEGKGVPADLHEKIRALQREYNAHRHEIPGAANASLP
                     DRWDLTEFLADRFAVAGTPADCVAQLRRAMGAGARQFMITGFVPDPRAFMRRWMKEVA
                     GAL"
     gene            4410..5027
                     /locus_tag="VFX87_16205"
     CDS             4410..5027
                     /locus_tag="VFX87_16205"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX4996524.1"
                     /translation="MIYEFRTYTLKPRSLAEVEKRYGEAYEYRKKYSPLAAFWHTEIG
                     PLNEIIHVWGYKDLAERARIRAEASKDPNWPPKIREFVIDQDVEVLTPFPFVPDITPG
                     TPGPIFEIRRYSLTAGSLPGVMKRWEGAIGERIKLSPLVLAGGVEFGGANRFVHIWAY
                     KSMDQRLAVREQARKAGIWPPPGGGDELLAQSSKIVMPSAFSPLK"
     gene            5041..5586
                     /locus_tag="VFX87_16210"
     CDS             5041..5586
                     /locus_tag="VFX87_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4996525.1"
                     /translation="MPPTKLAHVVFQTNRLPAMRDWYCAVLDGRVIYENPQLSFVTYD
                     DEHHRVAFIDLGPLAHRDLEGGDLRCRPTDQPGLHHVAFTFASMGELLGNYERLAARG
                     IRPFRSINHGPTTSMYYVDPDGNRVELQIDNFATAEEGQAWMLSPAFDRNPIGVDFEP
                     DDLVRRFQAGVPVAELVIRAD"
BASE COUNT          872 a         1890 c         2041 g          817 t
ORIGIN      
        1 gctgcccgcg atgatgaagc gctcgtccac gagctgcttc caggtgaggt tggccatcag
       61 cttctgcgcc tcgaggcgga gctgcgagag cgcgccgtac ttgatggtgt tcacggtgcg
      121 gtagcccggc gggtccgcga acggcgggaa cacgtgcaga caccggttga agaagtagag
      181 gcagtgctcg gcgtagagct cctcggcctc cgcgtcggtg tcggccacgc agatgatctg
      241 ggcgaaggcg gcgcggtagg gcgagtcgtc cttgccgcgc ttggccacgc ggtcccagta
      301 gccctcgagc agcgacttgc cgcggaggta gccgtagaag gagagatacg agtagttgta
      361 gtcgtagtcc aggcagaagt cccacgtctc gatggagccg ccgcccggga tgtagatggg
      421 cgggtggggc ttctggatcg gcttgggcca gcagttcacc cagcgaagct ggttgtactt
      481 gccgtcgaag gcgaagggct cgtcctcggc ccacgccttc atgatcatgt cgtgggcttc
      541 cttgtacttg tcgcgggtca gggcggggat ctggccgtag cagaagttgg tgtccatcgg
      601 cgtgccgacc ggaaagcccg cgaccagccg cccgcccgag atgacgtcga gcatggcgaa
      661 ctcctcggcc acccggatcg gcgggttgta gcccgcgatg gagttgccga tcacgcagat
      721 ggccgcgcgc gaggtccgcc gggcgaggcc ggccgcgatc aggttcgggg agggcatgag
      781 cccgtagccg ttctggtggt gctcgttgca gccgatgccg tcgaagccga gcgtctccgc
      841 gtactcgagc tggtccatgt actggtggta gacgaagtgg cccttcttcg ggtcgtagag
      901 cgtgctggga atgtccaccc acaccgagcg gttcttctcg cggaagtcgt ccggcaggta
      961 cggccagggc atcaggttga accaggtgaa tctcatgagt ccctccccac ggtgggttac
     1021 cggtgcgccg gagtgtataa cataatgccg ccatgcacgg agcccaggga ctcgatgcca
     1081 ggctgggccg acagctggtc ctggacgagc tgttcgatct cacgctctac cgctccctgc
     1141 agcgggtggc gcccgccggt ctccgaccga tcctcgagca gctcattccc atcgagacgc
     1201 gccaccacgc attctggcag gagttcttcg gcctgaagat cgcgacgctc gatcttccgc
     1261 ggcgcatgaa gctcgcgctc ctggtaggcc tggcgcggct gctcggagcg ccgggcattc
     1321 acctgatcct ggaggccatc gaggtctacg gggtccggaa gtacctcgcg gtgtggcggc
     1381 agtacgaagg ggggcctctg ggcgaggccg tccggagcat cctggaggac gagttcaagc
     1441 acgaggacgc ggtggtcatg gcggaatccg agcgccagat gaatccgcag cgggtgcgcg
     1501 acatcttcct cggtctgaac gacggcctcg tggagatcgt gggggcggtc agcggcttct
     1561 tcggggcgtt cgccgacgcg acgacggtgc tggtcgccgg gatgatggtg ggcgtcgcgg
     1621 gcgccctctc catgggggcg ggcgcctatc tcgcggggag ctccgaggcg gaggtccgtg
     1681 ataccgaggt ccgccggcgc cggtttctcg gggaggcgga tcccacggaa gggcgcgcgg
     1741 acacgccgct ggcctcgggc ctgcgggtcg gagtgagcta cgtgatcggc gcggtggtcc
     1801 cggtcctgcc ggtgctgttc ggcgcccgga cgctgctgcc ctcgctggtg gtggccggcg
     1861 cggtgatcgt catcgtctcc gcgatcctgg ccttcctctc cggcatgaac gtcaagcggc
     1921 ggatcctgat gaacctcgcc atcatcgccg cggcggtggc gatcagctat ctcatcggcc
     1981 gggtcgccca gtccgcgtgg ggcatcagcg tctgaccggc ctcaggtggt gagcgcgcgg
     2041 gcgcgccccc acatggccgc gcgccacctg accgcgatga gcgcggtgaa gaccagcccc
     2101 agcgcggcca gcaccgtgag ggtgagcggg tagccgatcc gctcgatcag cgcgccggag
     2161 gcgatgagcc cgatcggcag gccgtagacg cagagcgagc ggacgcccat cacccgcgcg
     2221 cggaagcccg ggcccgaggc ggtgagcagg gtcgcggtca tcgagatcat cgcgacgctc
     2281 tgcacgaagc cggtggccaa cagcacgagc aggcctccgc cgaggctcgg cacgtgggcg
     2341 aagacgagca ggagcccgta ccacagcacg gtgtgcacga gcgtgtagcg ctccgggtgg
     2401 gcgggcccgc cggtgaccac catcgcgatc gaggccagca gcgccccgaa cgagaacgcg
     2461 gcggccagcc agcccagccc ggtcgcgtcc acctggtaca cgcgctgcgc gatgtagggc
     2521 agcaggccgc ccgaggccgg gtacgcggtc aggttgatca ggaacgcgag ccacatcggc
     2581 gccaggagct gcggccggct caggatgtgc gcgaccccgt cctggagctc ccgccacccc
     2641 caatccctct gccccgagag ggggaggggg tagggtgagg gggcggcgaa gtctgggact
     2701 tgccgtgccc gcgccacccc gaacgtcagc gccaggctcg ccaggtagaa gcacgtcacg
     2761 aacacgtagg tgaggccgat ccccagcatc gtggacagcc cggcgcccgc cagcgcgccg
     2821 cccacccgcg cggaatccat ggtggcccgc gacatgccga gcgcgcccat caggtggccg
     2881 ggcggaatcg tctcgccgat cagggcgttg cgcatcacga ggtcgttcgg gcgcacgatg
     2941 ccgttgagcg cggccagcgc gatggcccag gcgggcgtca gccagccggt gaaggccagg
     3001 gccatcacgg tcaccgcgaa cgtggcgtag atcgcgcgca tcgcgcagag catgaggcgg
     3061 ccgcccaccc ggtcgcccag cacgccgaac atgggcgcgg cgagtgtgcc gaggaactgc
     3121 gcggagccga agagggtgag cagcagcacc gagccggtct ggaccatgac gtaccagccg
     3181 aggatgagcg tctccatctc gaacgcccag gaggtgaaga gatcggcggg ccactggaag
     3241 cggaagctgc ggacgcggaa ggcggcgagg atcaaagagc gccggcgacc tccttcatcc
     3301 accggcgcat gaaggcgcgc ggatccggca cgaagccggt gatcatgaac tggcgcgcgc
     3361 ccgcccccat cgcacgccgg agctgggcca cgcagtcggc gggcgtgccc gcgaccgcga
     3421 agcggtccgc caggaactcg gtgaggtccc agcgatcggg caggctcgcg ttcgcggcgc
     3481 ccgggatctc gtggcggtgg gcgttgtact cgcgctggag cgcgcggatc ttctcgtgta
     3541 gatcggcggg cacgcccttg ccctcgagcg tgaagcggaa ggcgtggttg gcgctggcgg
     3601 ccagcgccat cttgatcggc tcgatcgcgg cggcgcggct gccggcgagg ctggtcttgg
     3661 cgaaccacca gacgtcgacg tcctccagcg cgcggcccgc cgcgcgcgcg ccgcgctcga
     3721 tcgccgcgag cgagagcgcg atcacctcgg gcgtcaggcc gaggccgcag atcactccgt
     3781 cggccacgcg gccggccagc tcgagcgtgc gcgggccctc ggcggccagg tagatcggca
     3841 cccggcgcgt cgagcggtgc acgcgccagg cccgcccgcc gcggtccacc ggctcgcccg
     3901 cggtcagccg gccgagcgtc acgatcgagt cctcgaggcc ggccagcgtg gcgggcggcg
     3961 cgccgatcgt gtagatcgcg ctgtcgcccg agcccaggcc cagcacggcg cgcccgccgg
     4021 agagctcgtc cacgctggcg atggcgctcg cggtgaccac gaggtgccgg gtttgcgggt
     4081 tggtcacgag ggggccgagc ttcacgcgcc gggtgttgag cgcggccagc gtgagggcga
     4141 cgtagagctc gcggaagacc gactgcgagt cggccacccc gagccagtcg aagccggccg
     4201 cctccgcctc ggggcagagc tcgccgaaat cggtgagcga ttccggcagg aacacgatgc
     4261 cgaaccgcgc cgtggcggtg gtcatgatcg gccacaccct agcaccgacg acggcttgac
     4321 gtctccggaa ttggggcgca gacttcgcgg gtgccgccgt cgtggcggtg attgcgagcg
     4381 cgccccagcc ccagcatagg aggtcggcga tgatctacga gttccggacc tacacgctga
     4441 agccccgcag cctcgccgag gtggagaagc gctacggtga ggcctacgag taccggaaga
     4501 agtactcgcc gctcgccgcc ttctggcaca cggagatcgg gccgctcaac gagatcatcc
     4561 acgtgtgggg ctacaaggat ctcgccgagc gcgcccgcat ccgggccgag gcgtccaagg
     4621 atccgaactg gccgcccaag attcgcgagt tcgtcatcga ccaggacgtg gaggtcctga
     4681 cgccgttccc gttcgttccc gacatcacgc cgggcacgcc ggggccgatc ttcgagatcc
     4741 gccgctacag cctcacggcg ggctcgctgc ccggcgtcat gaagcgctgg gagggagcca
     4801 tcggggagcg gatcaagctc tcgccgctgg tgctcgccgg cggggtggag ttcggcggcg
     4861 cgaaccggtt cgtccacatc tgggcgtaca agagcatgga ccagcggctg gccgtccggg
     4921 agcaggcgcg caaggcgggc atctggccgc cgccgggtgg cggggacgag ctgctcgcgc
     4981 agtcgagcaa gatcgtcatg ccgtcggcct tctcgccgct gaagtaggag gggaacgacg
     5041 atgcctccga cgaagctcgc ccacgtcgtg ttccagacca accgcctccc cgcgatgcgg
     5101 gactggtact gcgccgtgct cgacggacgc gtgatctacg agaaccccca gctgtccttc
     5161 gtgacctacg acgacgagca ccaccgcgtg gccttcatcg atctgggccc gctggcccac
     5221 cgggacctgg aggggggcga cctccggtgc cggccgaccg atcagcccgg cctccaccac
     5281 gtcgcgttca cgttcgcctc gatgggcgag ctgctcggca actacgagcg gctcgccgcg
     5341 cggggcatcc gcccgttccg ctccatcaat cacgggccga cgacgtccat gtactacgtc
     5401 gaccccgacg gcaaccgcgt ggagctccag atcgacaact tcgccaccgc cgaggaaggg
     5461 caggcctgga tgctctcgcc cgccttcgac cgcaacccga tcggggtcga cttcgagccc
     5521 gacgacctgg tccgccgctt ccaggccggc gtgccggtcg ccgagctcgt catccgcgcc
     5581 gactagcccg gggtcaggtc ttacattccg acatttgtgc
//
LOCUS       DASUFA010000447         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_212041, whole genome shotgun sequence.
ACCESSION   DASUFA010000447 DASUFA010000000
VERSION     DASUFA010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212041"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /gene="secA"
                     /locus_tag="VFX87_16215"
     CDS             complement(<1..1735)
                     /gene="secA"
                     /locus_tag="VFX87_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX4996526.1"
                     /translation="MLNAIFRSIFGTKHERDVKRMRPVVAEIAALEPGLQALSDEALR
                     HRTTELRQRLADGAELDDLLPEAFAAVREAARRTVRMRHFDVQMMGGMVLHSGKIAEM
                     ATGEGKTLVATLPAYLNALPGLGTHVVTVNDYLARRDAQWMGPIYHALGLTVGVIQHE
                     MQYLYDPAYASSDIRLAALRPVTRKDAYAADITYGTNNEFGFDYLRDNMQFSLDDIVQ
                     REHYYAIVDEVDSILIDEARTPLIISGRDRNAESRAPLYEKVNRVIPRLKRAATIVEG
                     KLSEIEESAEGDFIVDEKARTVSLTDQGGAYCERLLGIENLSDPAHIETAHHIHQALK
                     AHHIFHKDVDYVVKDLETVDDQGRMVLQPQVVIVDEFTGRMMPGRRWSDGLHEAVEAK
                     EGLKIARENQTLATITLQNYFRMYDKLAGMTGTAATEAEEFAKIYELDVTVIPTNRTL
                     GRINFPDVVYKTEREKFDAVAAEIGERHAKGQPVLVGTVSIEKSERLSKLLKKHGIQH
                     QVLNAKYHEREAEIVAQAGREKTVTIATNMAGRGTDILLGGNPDFLSKEILRKRGLDP
                     AMAPPEQRVSAV"
     gene            complement(1745..>2339)
                     /locus_tag="VFX87_16220"
     CDS             complement(1745..>2339)
                     /locus_tag="VFX87_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX4996527.1"
                     /translation="LERLIQKEIRGLGMEHGRFRVALRREAAAAGELGSAEGGWRVGP
                     RGAETAELLLSANPGEDLRPLAKVVSGGELSRVMLAAKTVLAAADDVPVLVFDEVDAG
                     IGGRVADVVGQKLRASAEGRQVLCVTHLAPIAAYAGHHLLVEKRVARGATRTSVSPLR
                     DAARVDEVARMLGGERVTETSRRHARELLRGSAGGVR"
BASE COUNT          302 a          824 c          818 g          395 t
ORIGIN      
        1 cgacggcgga gacgcgctgc tccgggggcg ccatggcagg gtcgagcccg cgcttgcgca
       61 agatctcctt ggaaaggaaa tccgggttgc cgccgaggag aatgtcggtg ccgcgccccg
      121 ccatgttggt ggcgatggtg accgtcttct cccgccctgc ctgggccacg atctcggcct
      181 cgcgctcgtg gtacttggcg ttgagcacct ggtgctggat cccgtgcttc ttgagcagct
      241 tggagagccg ctccgacttc tcgatcgaca cggtgccgac cagcaccggc tgccccttgg
      301 cgtgccgctc cccgatctcg gcggccaccg cgtcgaactt ctcgcgctcg gtcttgtaga
      361 cgacgtccgg gaagttgatc cggcccagcg tgcggttcgt cgggatgacg gtgacgtcga
      421 gctcgtagat cttggcgaac tcctccgcct cggtggccgc ggtcccggtc atgcccgcga
      481 gcttgtcgta catgcggaag tagttctgca gcgtgatggt ggcgagggtc tggttctccc
      541 gcgcgatctt gaggccttcc ttcgcctcga ccgcctcgtg caggccgtcg gaccagcgcc
      601 ggcccggcat catgcggccg gtgaactcgt ccacgatcac cacctgcggc tgcagcacca
      661 tccgcccctg gtcgtccacc gtctccaggt ccttgaccac gtagtccacg tccttgtgga
      721 agatgtggtg ggccttgagc gcctggtgaa tgtggtgggc ggtctcgatg tgggcggggt
      781 cggagaggtt ctcgatgccg agcagtcgct cgcagtaggc cccgccctgg tcggtgagcg
      841 agacggtgcg cgccttctcg tccacgatga agtcgccctc ggccgactcc tcgatctccg
      901 agagcttgcc ctccacgatg gtggcggccc gcttgagccg cgggatcacc cggttcacct
      961 tctcgtagag cggggcccgg ctctccgcgt tgcggtcgcg ccccgagatg atgagcgggg
     1021 tccgcgcctc gtcgatcagg atcgagtcca cctcgtccac gatcgcgtag tagtgctcgc
     1081 gctggacgat gtcgtcgagg ctgaactgca tgttgtcgcg gaggtagtcg aagccgaact
     1141 cgttgttggt gccgtaggtg atgtcggccg cgtaggcatc tttccgcgtc accgggcgca
     1201 gggcggccag ccggatgtcg gaggaggcgt aggccgggtc gtagaggtac tgcatctcgt
     1261 gctggatcac gcccaccgtg agcccgaggg cgtggtagat cgggcccatc cactgcgcgt
     1321 cgcgacgggc caggtagtcg ttgaccgtga ccacgtgggt gcccaggccg ggcagcgcgt
     1381 tcaggtaggc gggcagcgtg gccaccaggg tcttgccctc gccggtggcc atctccgcga
     1441 tcttgccgga gtggagcacc atgccgccca tcatctgaac gtcgaagtgc cgcatgcgca
     1501 cggtccgccg cgccgcctcg cggaccgccg cgaacgcttc ggggagcagg tcgtcgagct
     1561 ccgccccgtc ggccagccgc tggcgcagct cggtggtgcg gtggcgcagg gcctcgtcgg
     1621 agagcgcctg caggcccggc tcgagcgcgg cgatctcggc gaccaccggg cgcatgcgct
     1681 tgacgtcgcg ctcgtgcttg gtgccgaaga tcgagcggaa gatggcgtta agcatgtccg
     1741 ggcctcatct tacaccaccc gccgagcccc gcaggagctc ccgggcgtgg cggcgcgagg
     1801 tttcggtgac ccgctccccg cccagcatgc gcgcgacctc gtccacgcgg gcggcgtccc
     1861 ggagcgggct cacggacgtg cgcgtggcgc cgcgggccac ccgcttctcg accagcaggt
     1921 gatgccccgc gtaggccgcg atcggggcca ggtgtgtcac gcagagcacc tggcggccct
     1981 ccgccgaggc gcggagcttc tggccgacga cgtcggccac gcgaccgccg atgcccgcgt
     2041 ccacctcgtc gaagaccagc accggcacgt cgtccgccgc ggccaggacc gtcttggcgg
     2101 ccagcatcac ccgggagagc tcgccgcccg agacgacctt ggcgagcggc cgcaggtcct
     2161 cgcccggatt ggccgagagc agcagctcgg cggtctcggc tccgcgcggc cccacccgcc
     2221 agcccccctc ggccgatccg agctcgcccg cggccgccgc ctcgcggcgc agggccacgc
     2281 gaaagcgccc gtgctccatc ccgagcccgc ggatctcctt ctggatgagc cgctcgagc
//
LOCUS       DASUFA010000448         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_682024, whole genome shotgun sequence.
ACCESSION   DASUFA010000448 DASUFA010000000
VERSION     DASUFA010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682024"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFX87_16225"
     CDS             <1..837
                     /locus_tag="VFX87_16225"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX4996528.1"
                     /translation="RSVRDLSGVRQPGAGALAIGAGTTLTAVARHPEVRARYAALATA
                     AGAVSTPQLRNMGTLGGNVCVDTRCNYYNQSYQWRKAVNFCMKKDGEICLVAPGSPRC
                     WAVSSSDTAPALWSLGASVRLAGPAGERVIPISALYQDDGIQYLTKQPGEIVTEIVLP
                     PAAGARSVYLKLRRRGSFDFPVLGVAATLHMDGETVRQARIVLGAVASLPREAPDAAR
                     LLVGERLTPELIDRAADAAYRPARPLDNTDLTHPYRKKMVRVYVARALRRLAGLPDEA
                     EA"
     gene            834..1382
                     /locus_tag="VFX87_16230"
     CDS             834..1382
                     /locus_tag="VFX87_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX4996529.1"
                     /translation="MTALGQRIKAFRAERALQQRQLAEKAGLTPSMLSQIESGRLTPS
                     LPTLGKLAAALGVSIGSLFEPAPAGRLLVSRKADYPVVSFDGTTEKWHVLGAGLFQGK
                     IRAVMSTLGPRSRGVKTDKVVIEPGQMKLFYVIEGKVALQYNGDRHMLEAGDSAYLDG
                     GTTHGWENVGSRIAKVLWVILG"
     gene            1463..>2050
                     /locus_tag="VFX87_16235"
     CDS             1463..>2050
                     /locus_tag="VFX87_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4996530.1"
                     /translation="MNERVVDFRTDPSRYRHWQLSFDGPVATLAMDVREDGGLVPGYE
                     LKLNSYDLGVDIELYDAIQRLRFEHPEVGAVVLTSGKERIFCAGANIRMLSQSSHGWK
                     VNFCKFTNETRSAIEDATTNSRQVWLTAVNGPCAGGGYELALATDWIIMSDDGSTTVA
                     LPEVPLLAVLPGTGGLTRLVDKRRVRRDRADFFCTL"
BASE COUNT          303 a          715 c          742 g          290 t
ORIGIN      
        1 cgcagcgtcc gggacctgtc cggcgtgcgg cagccgggcg cgggcgcgct cgcgatcggg
       61 gcggggacca cgctcaccgc ggtcgcgcgg caccccgagg tgcgcgcgcg gtacgcggcg
      121 ctggccaccg cggcgggcgc ggtctccacg ccgcagctcc gtaacatggg cacgctcggc
      181 ggcaacgtgt gcgtggacac gcgctgcaac tactacaacc aatcctacca gtggcggaag
      241 gcggtcaact tctgcatgaa gaaggacggc gagatctgcc tggtggcgcc ggggagcccg
      301 cgctgctggg cggtgtcctc ctcggatacc gcgcccgcgc tctggagcct gggggcaagc
      361 gtgcggctgg ccggccccgc cggcgagcgc gtgatcccga tctccgcgct ctaccaggac
      421 gacggcatcc agtacctcac caagcagccg ggggagatcg tcaccgagat cgtgctgcca
      481 cccgccgccg gcgcccgctc cgtctatctc aagctgcgcc gccgcggctc cttcgatttc
      541 ccggtgctgg gcgtcgcggc cacgctgcac atggacggcg agacggtgcg ccaggcgcgc
      601 atcgtgctgg gcgcggtggc gtcgctgccc cgggaagccc cggacgccgc ccgcctgctg
      661 gtcggcgagc gcctcacgcc cgagctgatc gaccgcgcgg ccgatgccgc gtaccggccc
      721 gcccggccgc tcgacaatac cgatctcacg cacccgtacc ggaagaagat ggtgcgcgtc
      781 tacgtggcgc gcgcgctgcg gcggctggcc ggcctgccgg acgaggcgga ggcgtgaccg
      841 cgctcggcca gcgcatcaag gccttccgcg ccgagcgggc cctgcagcag cgccagctcg
      901 ccgagaaggc cgggctcacc ccgagcatgc tgtcgcagat cgaatcgggg cgcctgaccc
      961 cgtcgctgcc gaccctgggc aagctggccg cggccctggg cgtgtccatc ggctcgctct
     1021 tcgagccggc cccggccggg cggctcctgg tgagccggaa ggccgactat ccggtggtgt
     1081 ccttcgacgg caccaccgag aagtggcatg tgttgggggc cgggctcttc cagggtaaga
     1141 tccgggcggt gatgtccacg ctcgggccgc gatcgcgggg cgtgaagacg gacaaggtgg
     1201 tcatcgagcc ggggcagatg aagctcttct acgtgatcga gggcaaggtg gccctgcagt
     1261 acaacggcga ccgccacatg ctcgaggcgg gcgacagcgc ctacctcgac ggcggcacca
     1321 cgcacggctg ggagaacgtg ggctcccgga tcgccaaggt gctgtgggtg atcctcggat
     1381 gacgtcagcc cacagccggt tgaaccaacc gggatggccc acggtcaaca tgcctcgggc
     1441 atgttgagga tgaagagggg ttatgaatga gcgagtagtc gacttccgga cggacccgag
     1501 ccggtaccgg cactggcagc tctccttcga cggcccggtg gcgacgctgg ccatggacgt
     1561 ccgggaggac ggcggcctcg tgcccggcta cgagctcaag ctcaattcct acgacctggg
     1621 cgtggacatc gagctctacg atgccatcca gcgcctgcgc ttcgagcatc cggaggtcgg
     1681 cgcggtcgtc ctcacctcgg gcaaggagcg gatcttctgc gcgggggcca acatccggat
     1741 gctgagccag tcctcccacg gctggaaggt gaacttctgc aagttcacca acgagacgcg
     1801 cagcgcgatc gaggacgcca cgacgaactc gcgccaggtc tggctcaccg cggtgaacgg
     1861 gccctgcgcg ggcggcggct acgagctggc gctcgccacc gactggatca tcatgtccga
     1921 tgacggctcc accacggtgg ccttgcccga ggtgccgctg ctcgcggtgc tgccggggac
     1981 cggcggcctg acgcggctgg tggacaagcg gcgggtgcgg cgcgaccgcg ccgacttctt
     2041 ctgcacgctg
//
LOCUS       DASUFA010000449         6328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_212926, whole genome shotgun sequence.
ACCESSION   DASUFA010000449 DASUFA010000000
VERSION     DASUFA010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212926"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VFX87_16240"
     CDS             <1..1105
                     /locus_tag="VFX87_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX4996531.1"
                     /translation="HYPFGLFAVPREDVLRIHASSGTKGKPTVVGYTRNDLRVWREVM
                     ARSLAAGGAKPGQLIQIAYGYGLFTGGLGFHDGAEHMGLTVVPVSSGNTLRQILLLQD
                     FRPQGLACTPSFALHIGESLREQGSDPRAIGLAYGLFGAEPWTEAMRAQLEALWGCPA
                     VDFYGLSEIIGPGVAGECVEARDGLHVNEDHFLPEIVDPRSGAPLPAGQEGELVLTGL
                     TKEALPMIRYRTGDITRLDPAPCRCGRTTVRMARVKGRTDDMLIIKGVNVYPSQLEAA
                     LLTVADLAPHYQLVVDRREAFPTLAVQVEPDERVVREWGGFEAAPARLAALSAQVAAC
                     LRGHLGLNPEIAVVAPKTIPRSEGKAVRVVERR"
     gene            1108..1932
                     /locus_tag="VFX87_16245"
     CDS             1108..1932
                     /locus_tag="VFX87_16245"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX4996532.1"
                     /translation="MAKRDEARLLARIEAGERIESPDEMTEAYRDNLIHLMTMQADSE
                     LAGGYGYVPWITKAPGVEEKHVVAQIVKDELRHATVMYGLLADLGVDVEAHVRAHDEV
                     FTMRIANDADIGTARITADKRVNIFYYPIDTWADFIFFNFCMDRGAGHQLEDVRACSY
                     GPWVRAIEGIFKEEKFHIRHGEFWVKRLAEDPNTRDEAQATLNKWYIRTMNIFGRPGS
                     AKNVLYRKYRLKLRDNDEVRQTFAREVAEKAGAVGLTLPEWNPVWDRLPEEAQIPG"
     gene            1962..2591
                     /locus_tag="VFX87_16250"
     CDS             1962..2591
                     /locus_tag="VFX87_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996533.1"
                     /translation="MALLALAVAASVAAQSNRQVKVLLEFKQQTQGSRQGVQGQGGMV
                     VEERRGAPRVRGRGGLAVEDTTTRVTRSSGLFTVVQDGGAGSIMVAQDVPYQQMAYYY
                     DYATGKGYVAQGVAWQRVGTALAVRPVILPGNKIRVTLTPVISYFTPGGGGTIEVVEA
                     ATDLIVPSGGRMQVGGSTGSLHAITRQILGYSSQQSSEETALTLTATIQ"
     gene            complement(2642..2983)
                     /locus_tag="VFX87_16255"
     CDS             complement(2642..2983)
                     /locus_tag="VFX87_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996534.1"
                     /translation="MIGGTLHLALIYLHPGQQDALRRYENLALPVFRRHGGRFERILS
                     PWRVAGGQPDPDEPDEIHLLRFEAAEGLDAVRRDPEMAALAPIRREIVRKALLLQVED
                     VPLERYFTPRS"
     gene            complement(3072..3716)
                     /locus_tag="VFX87_16260"
     CDS             complement(3072..3716)
                     /locus_tag="VFX87_16260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4996535.1"
                     /translation="MSELAEFLQSRGARFGLKIAGVVVALGLVGVAGWLWLRAEEARG
                     QAALAAAEAVQQGEGPLATAEGRQKAVGMLQAVLTQHGRSAAAAQAAYQLGNLQYQAG
                     DYAAARAAYEVALTKGAGGSLRTLSASGVGYTWEAQKNYANAVTAYEAAARGLGPKQF
                     FFEEALLDLARAQTLAGKPAEARATYERLLKDAPETRRAEDIRGRIAALAPAAK"
     gene            complement(3713..4159)
                     /locus_tag="VFX87_16265"
     CDS             complement(3713..4159)
                     /locus_tag="VFX87_16265"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX4996536.1"
                     /translation="MGALGRTLRYLGLGGGDSDSLAADLAAHREARSEPAGLGAGLTL
                     KGEVAGEGDFHIAGRFEGGINVTGRVLVGEGADVDANINARAIVVGGTVRGNLSAATR
                     VEILPTGVLTGTLKTGSFSAADGAAVKGEIWIERPEGADPVEERRR"
     gene            complement(4166..4414)
                     /locus_tag="VFX87_16270"
     CDS             complement(4166..4414)
                     /locus_tag="VFX87_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996537.1"
                     /translation="MRRVKRMLKWRASAVTGLIAVLALVSLPVEVTIGLIEHPVVPPG
                     RLASLLLIGSCLLLVGSGLGLRREEAARAGARRSTPSA"
     gene            4509..4652
                     /locus_tag="VFX87_16275"
     CDS             4509..4652
                     /locus_tag="VFX87_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996538.1"
                     /translation="MLDSAGRARLLHVAIWLATLFFTAMLFVPLLREALRRVTELPSS
                     GHF"
     gene            4652..5044
                     /locus_tag="VFX87_16280"
     CDS             4652..5044
                     /locus_tag="VFX87_16280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4996539.1"
                     /translation="MAGLLSAARLLVVMEDEHARGLLVAVLRADGYTVDAAATRADAL
                     SLIDRHAYALVVSALRMPELDGPALSRALEARWPLEMPALIFVTQPAFTPDLARFLME
                     SAAPLLLWPARPADISRMVARALAPAPA"
     gene            complement(5051..5257)
                     /locus_tag="VFX87_16285"
     CDS             complement(5051..5257)
                     /locus_tag="VFX87_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996540.1"
                     /translation="MHWLSSRSVAIVWLLAALSLVGWLIVNLDMAGWIILVVTVGLMA
                     FELTRRRDGQGARPKARIPDKPTG"
     gene            complement(5314..6153)
                     /locus_tag="VFX87_16290"
     CDS             complement(5314..6153)
                     /locus_tag="VFX87_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4996541.1"
                     /translation="MKRAGSRKATARAGLFLTGIRRAGALPEPAPFPFTLPLFRDLER
                     LDFRAPVTFLVGENGCGKSTLLEGLAAGAGAVAAGGRDLERDPTLAGARAFARAFRFS
                     RIRHPRATLFIRAEDVFGFTLRVGGEIAGLRAMEDELRASRPEGSWGQRLAMGAAAKE
                     RAALARRYGAAPDARSHGETFLHLLQERLVPRGLYFLDEPETPLSPTRVLALVALLRD
                     RVALDCQFVIATHSPILMALPRADIRLLEDGAIAPVAYADVEHVRITRAFLDDPESFL
                     RRL"
BASE COUNT          907 a         2263 c         2295 g          863 t
ORIGIN      
        1 gcactacccg ttcgggctct tcgcggtgcc gcgcgaggac gtgctccgca tccatgcctc
       61 ctcgggcacc aagggcaagc ccacggtggt aggctacacg cggaacgacc tccgggtctg
      121 gcgcgaagtc atggcccgct cattggccgc cgggggcgcg aagccgggcc agctcatcca
      181 gatcgcctac ggctacggcc tcttcacggg cggcctgggc ttccacgacg gcgccgagca
      241 catggggctc accgtggtgc cggtctcctc gggcaacacg ctccgccaga tcctgctgct
      301 ccaggacttc cggccgcagg gcctggcctg cacgccgtcc ttcgccctcc acatcggcga
      361 gagcctgcgg gagcagggga gcgatccgcg cgcgatcggc ctggcgtacg ggctcttcgg
      421 cgccgagcca tggaccgagg cgatgcgcgc gcagctcgag gcgctgtggg gctgcccggc
      481 ggtggatttc tacgggctca gcgagatcat cggccccggc gtggcgggcg agtgcgtgga
      541 ggcccgcgac ggcctgcacg tcaacgagga ccacttcctg cccgagatcg tcgacccgcg
      601 gagcggggcg ccgctgcccg ccgggcagga gggggagctg gtgctgactg ggctcaccaa
      661 ggaagccctc ccgatgatcc gctaccgcac cggcgacatc acgcggctcg atcccgcgcc
      721 ctgccgctgc gggcggacca ccgtgcgaat ggcgcgcgtc aaggggcgca ccgacgatat
      781 gctgatcatc aagggggtca acgtctatcc ctcccagctc gaggcggcgc tcctgacggt
      841 ggcggatctg gccccgcact accagctggt ggtggatcgg cgggaggcct ttccgacgct
      901 cgcggtgcag gtggagccgg acgagcgcgt cgtgcgagag tggggcgggt tcgaggccgc
      961 acccgcccgg ctggccgcgc tgtccgcgca ggtggccgcg tgcctgcgcg gccatctcgg
     1021 gctcaacccg gagatcgcgg tcgtggcgcc gaagaccatt ccgcgcagcg agggcaaggc
     1081 ggtgcgggtc gtggaaagga gatagtcatg gcaaagcgcg acgaagcccg gttgctggcc
     1141 cgcatcgagg cgggggagcg catcgagtcc cccgacgaga tgacggaggc gtaccgcgac
     1201 aacctcatcc acctcatgac catgcaggcc gactcggagc tggcgggcgg ctacggctac
     1261 gtgccgtgga tcaccaaggc gcccggggtg gaggagaagc acgtggtcgc ccagatcgtg
     1321 aaggacgagc tccgccacgc caccgtgatg tacggcctcc tcgccgacct cggcgtggac
     1381 gtggaggcgc acgtgcgggc gcacgacgag gtcttcacga tgcggatcgc caacgacgcc
     1441 gacatcggca ccgcgcgcat caccgccgac aagcgggtca acatcttcta ctatccgatc
     1501 gacacctggg ccgatttcat cttcttcaac ttctgcatgg atcgcggcgc gggccaccag
     1561 ctcgaggacg tgcgcgcctg ctcctacggg ccctgggtgc gcgcgatcga ggggatcttc
     1621 aaggaggaga agttccacat ccgccacggc gagttctggg tgaagcgcct cgccgaggac
     1681 cccaacaccc gcgacgaggc ccaggccacg ctcaacaagt ggtacatccg caccatgaac
     1741 atctttgggc gcccgggctc cgccaagaac gtgctctacc ggaagtaccg cctcaagctg
     1801 cgcgacaacg acgaggtccg ccagaccttc gcccgcgagg tcgcggagaa ggcgggggcg
     1861 gtcgggctca cgctgcccga gtggaatccg gtctgggacc gcctgcccga agaggcgcag
     1921 atccccgggt gatcccgccg agccgacttc tcgtcgtcgc gatggcgctg ctcgcgttgg
     1981 ccgtcgccgc gtccgtcgcg gcgcagagca accgccaggt gaaggtgctg ctggagttca
     2041 agcagcagac ccagggcagc cgccagggcg tgcaggggca gggcggcatg gtcgtggagg
     2101 agcgccgcgg cgccccccgg gttcgcggcc ggggcggtct cgcggtggag gacaccacca
     2161 cgcgcgtcac gcgctcctcc gggctgttca cggtggtgca ggacggcggc gccggctcga
     2221 tcatggtcgc ccaggacgtc ccgtatcagc agatggcgta ctactacgac tacgcgaccg
     2281 gcaagggcta cgtcgcccag ggcgtcgcat ggcagcgcgt gggcaccgcg ctcgcggtgc
     2341 gtccggtgat cctccccggc aacaagatcc gggtgacgct gacgccggtg atcagctact
     2401 tcacgcccgg gggcgggggc acgatcgagg tcgtggaggc cgcgaccgac ctgatcgtgc
     2461 ccagcggggg gcggatgcag gtcggcggca gcaccggcag cctccacgcg atcacccggc
     2521 agatcctcgg ctacagctcc cagcaatcgt cggaggagac ggcgctgacc ctcaccgcga
     2581 ccatccagtg acccgctatc cggcagcgat cacttcgtgg tgcccggcct gaagccggcg
     2641 ctcagcttct gggcgtgaag taccgctcca gcggcacgtc ctcgacttgc agcagcagcg
     2701 ccttcctgac gatctcccgg cggatcgggg ccagcgcggc catctccgga tcgcgccgga
     2761 cggcgtccag gccctccgcc gcctcgaacc gcagcagatg gatctcgtcc ggctcgtcgg
     2821 gatcgggctg tccgccggcg acccgccacg ggctcaggat ccgctcgaag cggccgccgt
     2881 gccggcggaa gacgggcagg gccaggtttt cgtatcggcg aagggcgtcc tgctggccgg
     2941 ggtggagata gatgagcgcc aggtggagcg tgccgccgat catgccggcc gaacgttact
     3001 taacataatg ttgcttatca gactatccgg ccgggccgcg gcggccgtgg gggtctagtc
     3061 ggattggctc tctacttcgc ggccggagcg agcgcggcga tgcgaccgcg aatgtcctcg
     3121 gcgcgccggg tctcgggcgc atccttgagc aggcgctcgt aggtcgcccg cgcctccgcg
     3181 ggcttgccgg ccagcgtctg cgcgcgcgcc aggtccagca gcgcctcctc gaagaagaac
     3241 tgcttggggc cgagcccgcg cgccgccgcc tcgtacgcgg tcaccgcgtt ggcgtagttc
     3301 ttctgagcct cccaggtgta gccgacgccc gaggcgctca gcgtccggag gctgccgccg
     3361 gcgcccttgg tcagcgcgac ctcgtaggcg gcccgcgcgg ctgcgtagtc gccggcctgg
     3421 tactggaggt tgccgagctg gtaggcggcc tgggccgctg ccgccgaccg gccgtgctgg
     3481 gtcaggacag cctggagcat gccgacggcc ttctgccgtc cctccgccgt cgccaggggc
     3541 ccctcgccct gctggaccgc ctcggcggcg gccagcgccg cctggccgcg agcctcctcg
     3601 gcgcgcagcc agagccagcc cgccacccct acgaggccca gcgcgacgac cacgccggcg
     3661 atcttgagcc caaaccgcgc accgcgggac tggaggaact cggccagctc gctcaccggc
     3721 ggcgctcctc gacgggatcc gcgccctcgg gccgctcgat ccagatctcg cccttgaccg
     3781 cggcgccatc cgcggccgag aagctgccgg tcttgagcgt gccggtcagc acgccggtcg
     3841 ggaggatctc cacgcgggtc gcggccgaga ggttgccgcg cacggtgccg cccaccacga
     3901 tggcgcgcgc attgatgttg gcatccacgt cggccccttc gcccaccagc acgcggcccg
     3961 tcacgttgat gcccccctcg aagcgtcccg cgatgtggaa gtcgccctcg cccgccacct
     4021 cgcccttgag ggtcagtccg gcgccgagtc cggcgggctc gctgcgcgcc tcacggtggg
     4081 cagccaggtc ggcagccagc gagtccgagt cgccgccgcc caggcccagg taccggagcg
     4141 tgcggcccag cgcgcccacg tgctcctacg cgctcggggt cgaccgccgg gcgcctgccc
     4201 gggcggcctc ttctcgacgc aggccgagac cggagcccac gagcaggagg caggagccga
     4261 tcagcaggag cgacgccagg cggcccggcg ggaccacggg atgctcgatg agcccgatcg
     4321 tgacctcgac gggcaggctg accagggcca gcaccgcgat gaggccggtg actgcgctcg
     4381 cccgccactt gagcatccgc ttgacccgtc gcatggcgcg tagtgtagcc accgagatac
     4441 cggatagcaa ccggaagcgg cgccgaccgc ttgacggcgg ccggatgcgg gactactctc
     4501 cagttcttat gctcgacagc gccgggcgcg cgcgtcttct ccacgtggcc atctggctgg
     4561 ccacgctgtt cttcaccgcg atgctcttcg tgccgctcct gcgcgaggcg ctgcgccgcg
     4621 tgaccgagct gccgtccagc ggacatttct gatggcgggt ctgctgtcgg cggcgcgcct
     4681 cctggtcgtg atggaggacg agcacgcccg cgggctcctg gtcgcggtgc tgcgggccga
     4741 cggctacacg gtggacgcgg ctgcgacccg cgcggacgcc ctgagcctga tcgaccggca
     4801 cgcctacgcg ctggtggtga gcgcgctccg catgcccgag ctcgacgggc cggcgctgtc
     4861 gcgggcgctg gaggcgcggt ggccgctcga gatgcccgcg ctcatcttcg tgacccagcc
     4921 ggccttcact cccgacctcg cccgcttcct gatggagtcg gcggccccgc tcctgctgtg
     4981 gccggcccgc ccggccgata tcagccggat ggtcgcccgc gcgctcgccc ccgcccccgc
     5041 ctgagcgggg ctagccggtc ggcttgtcgg ggatgcgggc cttgggccgc gcgccctgcc
     5101 cgtcgcgtcg ccgggtgagc tcgaaggcca tgagccccac cgtgaccacg aggatgatcc
     5161 agcccgccat gtcgaggttg acgatgagcc acccgacgag gctgagcgcg gcgaggagcc
     5221 acacgatggc gacggagcgg gagctgagcc agtgcatcgc ggccaattat cccggcggcg
     5281 ggcgctcaag tcaaatccgg gcgctgcggg cgctcagagc cggcggagaa agctctccgg
     5341 atcgtcgagg aacgcgcgcg tgatgcgcac gtgctcgacg tcggcgtacg cgacgggcgc
     5401 gatcgcgccg tcctcgagca gccggatgtc ggcccgcggc agcgccatga ggatcggcga
     5461 atgggtcgcg atgacgaact ggcagtccag ggccacgcga tcgcgcagga gggcgaccag
     5521 cgcgagcacg cgcgtgggcg acagcggcgt ctcgggctcg tcgaggaagt agaggccgcg
     5581 cggcaccagt cgctcctgga gcaggtggag aaaggtctcg ccgtgggagc gcgcgtccgg
     5641 cgccgccccg tagcggcggg ccagcgcggc ccgctccttg gccgccgcgc ccatcgcgag
     5701 ccgctggccc cacgagccct cgggccggga ggcgcgcagc tcgtcctcca tcgcgcgcag
     5761 gcccgcgatc tcgccgccca cccgcagggt gaagccgaag acgtcctccg cgcggatgaa
     5821 gagggtggcg cgcggatggc ggatgcgcga gaagcggaag gcccgggcga aggcccgggc
     5881 gccggcgagg gttggatcac gttccaaatc gcgcccgccc gccgccaccg cgccggcgcc
     5941 cgcggccagg ccctccagca gggtggactt gccgcagccg ttctccccca ccaggaaggt
     6001 cacgggcgcc cggaagtcga ggcgctcgag atcgcggaag agcggcagcg tgaacggaaa
     6061 cggcgcgggc tccggcaagg cgccggcgcg gcggatgccg gtgaggaaga gcccggcccg
     6121 cgcggtggcc ttgcgggagc ccgcccgctt cacgcggatc ggctagcgaa gctccgggat
     6181 cacccagagc ggccgctcgc cgcgggccac gcgctggacg ttgtcgaacg cgttccggaa
     6241 ggccttgaac cagttgtccc aggtggggcc cgcggtgtgc ggggtgaacg tcacgttctc
     6301 gagcgcaaac agcgggtggt tcgcgatc
//
LOCUS       DASUFA010000450         7778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_683496, whole genome shotgun sequence.
ACCESSION   DASUFA010000450 DASUFA010000000
VERSION     DASUFA010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683496"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..681)
                     /locus_tag="VFX87_16295"
     CDS             complement(127..681)
                     /locus_tag="VFX87_16295"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4996542.1"
                     /translation="MTARKPLLDALVAMLTPRDVLVSCLGANARYLPHMTVPCPVFAL
                     CDSMSAAIPLGLGMALQRPDRHVVALEGDGSLLMSPNVLATVAAAAPPNLTMLLWVNG
                     RYESSGGQALPAVSVDWAVLARAAGIAHVASVGDVGGLREAFGRARAAGAPALVVVEI
                     AFDPAEAIPPYSERPDEIRARFRL"
     gene            complement(678..1172)
                     /locus_tag="VFX87_16300"
     CDS             complement(678..1172)
                     /locus_tag="VFX87_16300"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4996543.1"
                     /translation="MTGLEQIVAGLEAAQVSIVASVPDTWIGRLMAGVRRSPRLRAVD
                     VAREEEAVAVACGANLAGGRGAVLIQNAGLLNCGGILAGLVELYHLPCFFIVSLRGDH
                     RDPVYYHAPKGRVTEETLRAWRLPYARADRRADLAAQVRQGVEFCVESRGPFVLLISG
                     EDLA"
     gene            complement(1169..2248)
                     /locus_tag="VFX87_16305"
     CDS             complement(1169..2248)
                     /locus_tag="VFX87_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996544.1"
                     /translation="MARAVVELVGCTRDYGGVHAVAGLDLVVHEGELLSLLGPSGCGK
                     TTTLNLIAGFVPPTTGRVRIDGQDVTDHPAHQRKLGVVFQSYALFPHLSVFENVAFGL
                     RERRTPRAEIERRVRTALALVRLDTRAEARPAHLSGGMQQRVALARALVYEPRVLLLD
                     EPLAALDKKLREEMRSELREIQRSVGITTIFVTHDQGEALGLSDRIAVMLGGRIEQLG
                     APREIYERPATRFVAEFIGASSVLRGRATAADQVTLAGGEAVRVLSVPGLRAGEAVEV
                     AIRPERIRLAGGPGENVMDGRVTGVVYQGVQTDLTVEIGGGQRLFVFVPEPAPVALAP
                     GQTLRLHLPPDAFMRLGSAGAEAGR"
     gene            complement(2259..3035)
                     /locus_tag="VFX87_16310"
     CDS             complement(2259..3035)
                     /locus_tag="VFX87_16310"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996545.1"
                     /translation="MFRLVVLALLAFMTLPTVVVIAVSFNPTAILSFPPNGLSLRWYV
                     NVVTYPQFQRAMWNSLLVTAGAVALALPIGTAAALALARGRVRGASIWATLLLSPLVV
                     PGVAAGLGFLILGAALGLLRSRSVLIAAHVALVLPFVVRSVWVSVQNLDPTLERAAAS
                     LGATPWRAFRRVTLPLLRPGLLAAGLFAVIVSLNEFVVSLFISTRLTEILPVAMFSYV
                     VNYTDPTIAALSTLFIVGTFVVVWLADRFLDLGGVFLHHR"
     gene            complement(3035..3829)
                     /locus_tag="VFX87_16315"
     CDS             complement(3035..3829)
                     /locus_tag="VFX87_16315"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4996546.1"
                     /translation="MRLLLAPAVLVVAVFAAALSALLRYSVLEFVPGSLQTGGLTVAN
                     FTGTMSRQYLGAVWDTLVLSAATTALTLVASYPVAYALARTHSARVRSALLVLVLAPF
                     FTGAIVRTYAWVLVLGNTVVTWPVPMLFTPRGVLVGLVHFSMPTMILLLAAAISHIDP
                     VYERAAASLGASPARVFRRVTLPLSLPGIVSGSVIVFAWTLSAFATPELLGGGRVKMI
                     ANVVRDLALDSFNWPGGAAFALMALVITLALLAGAGKLTAVADRGA"
     gene            complement(3832..4866)
                     /locus_tag="VFX87_16320"
     CDS             complement(3832..4866)
                     /locus_tag="VFX87_16320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4996547.1"
                     /translation="MLARLAVVTLLALCLPFGPVAPAHAQNSLVVSVWGGNWKDTVDR
                     VIGKAFTAKTGMPVEFEVGGTIDRLAKARVAKGSPLVDITFTTSHVGRLYVSDGLFAP
                     LDMAKLPNAKAAAREALRSEYQMGAWSYVYTIVYRPDLVKEEITRWSDLWKPSLKGKI
                     AMPDFDPSHIITIAALMEGGNEATWQKGQERLKALKPSIVAFFSTDAQSQDLMKTGQA
                     PVQVMLSVNAYHLQEQGIAVKLVQPKDYPGVVGIDCMSVMAGTKKADAALAFIDLALS
                     KEIQTELAKALKAGPVNGGATVPAAFQGQPGIFLTPSEWKERGYILNDEVRARNLPAW
                     REWFTANIVK"
     gene            complement(4878..5366)
                     /locus_tag="VFX87_16325"
     CDS             complement(4878..5366)
                     /locus_tag="VFX87_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4996548.1"
                     /translation="MNKAIRVTVNGQAHERAVEVRATLADFLREDLDLTGTHVGCEHG
                     VCGACTVLLDGEPVRACLMLAVQADGQRVSTIEGVAPADGLHPIQEAFWAKHGLQCGY
                     CTPGIIMSACAFLRERPAPSHEEIREMLAGHLCRCTGYHFIVEAIEAAARALESPPTR
                     TP"
     gene            complement(5363..6235)
                     /locus_tag="VFX87_16330"
     CDS             complement(5363..6235)
                     /locus_tag="VFX87_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX4996549.1"
                     /translation="MKPAPFDYVAPTTLDDAIGALATAGDDAKILAGGQSLIPMLALR
                     LARPSVLVDINRLRGLDGIREASGILEIGALVRQRALERWAAGRSPLFAEVLHAVAHP
                     PIRNRGTVVGSIVLADPASELPAVLLCLEGVVVARGPRGERMIPADRFYRAPLTTALD
                     GHELATAVRFTLPAAGSGWGFAEVSRRHGDFALVGAVAVLTCAADGTVARARLAFFGV
                     GGTPVRGAAAERALEGRAPTPALIGEAARAAAAGLSPDGDIHATASYRRRVAATLAER
                     TLTAALARCGGGLS"
     gene            complement(6232..>7778)
                     /locus_tag="VFX87_16335"
     CDS             complement(6232..>7778)
                     /locus_tag="VFX87_16335"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4996550.1"
                     /translation="AAILARRPVKWTEDRREHMMGSAHARAQVHDIEIAARRDGTMLA
                     VRDRIWVDLGAYNSWGIVLPYNTVAHLLGPHRVANLDVECLGVVTTKTPNAPYRGAGR
                     PETVFAMDRIVDCLARELAMDPAELRRRNFLSAADLPYELRIPYRDGNPLVYDSGDFK
                     AGLEAALRAVGYDALRAEQARLRERGVHRGIGISSYVEGTAIGPYEGATVRLDVSGHA
                     VVATGACCQGQGHETSFAQIAADALGIPLEAVTIVGGDTAAIPFGVGTFASRSAVNAG
                     SSIHEAAGRVKDKLVAAAAALLEAAPADVELADGMVSVRGAPASAIPISKVIQAAIPT
                     FAKPGVAPADFEATVYHHQPTVTYTSATHVAVVDVDTGTGAVKLLRYLVAHDCGTIIN
                     PVIVEGQIHGGVAQGVGGGLLEEMVYDEQAQLLTGTFMDYLVPTAMELPPIETVHLEY
                     PSPRNPLGLKGIGEGGAISPPAAIANAVEDALAPFGVRVTRTPLGPSVVLGLLDQAAA
                     RGGRPA"
BASE COUNT         1164 a         2876 c         2777 g          961 t
ORIGIN      
        1 ccgggcatct ggaaggtcgt cggcgggtgc tccacgggcg cctccggctt ctcgacgggc
       61 tacggcacgg acggctacgg ctccgtcggc ggcgtcctca tccggtgagg tagccgcgcg
      121 gcggcgctag agccggaagc gcgcgcggat ctcgtcggga cgctcggagt agggcgggat
      181 cgcctcggcc ggatcgaacg cgatctcgac caccaccagc gcgggcgccc ccgcggcccg
      241 cgcgcgcccg aacgcctcgc gcagcccccc gacgtcgccg accgaggcca cgtgggcgat
      301 acccgcggcg cgggccagca cggcccagtc cacgctcacc gcgggtagcg cctggccgcc
      361 ggacgattcg tagcggccgt tcacccagag cagcatcgtg agattcggcg gcgcggccgc
      421 cgccacggtg gccagcacgt tggggctcat gaggagcgag ccgtcgccct cgagcgcgac
      481 cacgtggcgg tcgggccgct ggagcgccat gccgagcccc agcgggatgg ccgcgctcat
      541 cgaatcgcac agcgcgaaga ccggacacgg cacggtcatg tgcggcaggt agcgggcgtt
      601 ggcgccgagg cacgagacga gcacgtcgcg cggcgtcagc atcgccacca gcgcgtccag
      661 cagcggcttg cgcgcggtca cgcgagatcc tccccgctga tcagcagcac gaaaggcccg
      721 cgcgactcga cgcagaactc gacgccctgc ctcacctggg cggccaggtc ggcccgccgg
      781 tccgcgcgcg cgtagggcag ccgccaggcg cgcagcgtct cctcggtcac gcggcccttg
      841 ggcgcgtggt agtagaccgg atcgcgatga tcgccgcgca gtgacacgat gaagaagcat
      901 ggcagatggt agagctccac cagcccggcc aggataccgc cgcagttgag gagccccgcg
      961 ttctgaatca gcaccgcgcc gcgccctccg gccaggttgg cgccgcaggc caccgccacc
     1021 gcctcctcct cgcgggccac gtccaccgcg cggagccgcg gcgagcggcg cacgcccgcc
     1081 atcagccggc cgatccaggt gtctggcacc gaggcgacga tcgagacctg ggccgcctcg
     1141 agccccgcga cgatctgctc gagcccggtc accggccggc ctcggcaccc gcggacccga
     1201 gccgcatgaa ggcgtccggc ggcaggtgca ggcgcagcgt ctggcccggc gcgagcgcga
     1261 ccggggcggg ctcgggcacg aacacgaaga ggcgctggcc gcccccgatc tcgacggtga
     1321 ggtcggtctg cacgccctgg tacaccacgc cggtgacgcg tccgtccatg acgttctccc
     1381 ccggcccccc ggcgagccgg atgcgctccg gccggatggc cacctcgacc gcctcgcccg
     1441 cccgcagccc gggcacgctc agcacccgga ccgcctcgcc ccccgcgagc gtcacctggt
     1501 ccgcggcggt ggcgcggccg cgcagcacgc tggaggcgcc gatgaactcc gccacgaagc
     1561 gggtcgccgg ccgctcgtag atctcgcgcg gggcgccgag ctgctcgatg cgcccgccga
     1621 gcatcaccgc gatgcggtcg gagagcccga gcgcctcgcc ctggtcgtgg gtgacgaaga
     1681 tcgtggtgat cccgaccgag cgctggatct cccgcagctc gctccgcatc tcctcgcgca
     1741 gcttcttgtc gagcgcggcg agcggctcgt ccagcagcag cacgcgcggc tcgtacacga
     1801 gcgcccgggc cagggccacc cgctgctgca tgccccccga gagatgggcc gggcgcgcct
     1861 ccgcgcgcgt gtccaggcgc accagggcca gcgccgtccg cacccgccgc tcgatctccg
     1921 cgcggggtgt ccggcgctcg cgcagcccga acgccacatt ctcgaacacc gagaggtgcg
     1981 ggaagagggc gtaggactgg aacaccaccc cgagcttgcg ctggtgcgcc gggtggtcgg
     2041 tgacgtcctg gccgtcgatc cggacgcgtc cggtggtcgg cggcacgaat cccgcgatca
     2101 ggttgagggt ggtggtcttg ccgcagcccg acgggcccag cagcgagagc agctcgccct
     2161 cgtggacgac caggtcgagc ccggcgaccg cgtggacgcc cccgtagtcg cgggtgcagc
     2221 cgaccagctc gaccactgcg cgggccatca gggtcgcctc accggtgatg gaggaacacg
     2281 ccgcccaagt ccaggaaccg gtcagcgagc cagacgacca cgaaggtgcc cacgatgaag
     2341 agcgtggaga gcgccgcgat ggtcggatcg gtgtagttga ccacgtagct gaacatggcc
     2401 accggcagga tctcggtcag ccgcgtcgag atgaacagcg agaccacgaa ctcgttcagc
     2461 gacacgatca ccgcgaagag ccccgccgcg aggaggcccg ggcgcagcag cggcagcgtc
     2521 acccgccgga acgcccgcca gggcgtggcg cccaggctcg ccgccgcccg ctccagcgtc
     2581 ggatccagat tctgcacgct cacccacacc gagcgcacca cgaacggcag caccagggcc
     2641 acgtgggccg cgatcagcac gctccggctg cgcagcaggc cgagcgcggc ccccaggatc
     2701 aggaagccca ggcccgccgc gaccccgggc accacgagcg gcgagaggag cagcgtggcc
     2761 cagatcgagg ccccgcgcac ccgcccgcgc gccagcgcca gcgccgccgc ggtgccgatg
     2821 ggaagggcca gcgcgaccgc gcccgcggtg accagcaggc tgttccacat cgcacgctgg
     2881 aactggggat aggtgaccac gttcacgtac cagcgcagcg acagcccgtt cggcgggaac
     2941 gacaggatcg cggtcgggtt gaacgagacc gcgatcacca cgacggtcgg cagcgtcatg
     3001 aacgcgagca gggccagcac gaccagccgg aacatcaggc gccccggtcg gcgaccgcgg
     3061 tgagcttccc cgcgcccgcc agcagcgcca gcgtgatcac cagcgccatc agcgcgaagg
     3121 ccgcgccgcc gggccagttg aaggagtcga gcgccaggtc gcgaaccacg ttggcgatca
     3181 tcttgacccg cccgccgccc agcagctcgg gggtggcgaa tgcgctcagc gtccaggcga
     3241 agacgatcac cgagcccgaa acgatgcctg gtagggacag cggcagggtc acgcgccgga
     3301 acacgcgggc cgggctcgcg cccaggctgg ccgccgcgcg ctcgtacacc ggatcgatgt
     3361 ggctgatcgc ggcggccagc agcaggatca tggtcggcat cgagaagtgc acgagcccca
     3421 ccagcacccc ccgcggcgtg aacagcatcg gcaccggcca ggtgaccacc gtgttgccca
     3481 ggaccagcac ccaggcgtag gtgcgcacga tggctccggt gaagaacgga gccaggacca
     3541 gcacgaggag ggccgagcgc acgcgggccg aatgcgtgcg cgcgagcgcg taggccaccg
     3601 ggtaggaggc gaccagcgtc agcgcggtgg tggccgcgct cagcaccagc gtgtcccaca
     3661 ccgcgccgag atactgccga ctcatggtgc cggtgaagtt ggcgacggtc aggccgccgg
     3721 tctgcagcga ccccggcacg aactcgagca cgctgtagcg cagcagcgcg gagagcgcgg
     3781 ccgcgaatac cgcgacgacg agcacggccg gggccaggag caggcgcacc gctacttgac
     3841 gatgttggcg gtgaaccact cgcgccaggc cggcaggttc cgggcccgca cctcgtcgtt
     3901 caggatgtaa ccccgctcct tccactccga cggggtcagg aagatgcccg gctggccctg
     3961 gaaggcggcg ggcacggtgg cgccgccgtt caccggcccc gccttgagcg ccttcgcgag
     4021 ctcggtctgg atctccttgg acagggccag gtcgatgaac gccagggcgg catccgcctt
     4081 cttggtgccg gccatcaccg acatgcagtc gatccccacg acgcccgggt agtccttggg
     4141 ctggaccagc ttgaccgcga taccctgctc ctggaggtga taggcgttga ccgagagcat
     4201 cacctgcacc ggcgcctggc cggtcttcat gaggtcctgg ctctgggcgt cggtcgagaa
     4261 gaaggccacg atgctgggct tgagtgcctt gagccgctcc tggcccttct gccacgtcgc
     4321 ctcgttgccg ccctccatca gcgccgcgat cgtgatgatg tgcgagggat cgaagtcggg
     4381 catcgcgatc ttgcccttga gcgagggctt ccagagatcc gaccaccgcg tgatctcttc
     4441 cttcacgagg tccggacgat agacgatcgt gtacacgtag ctccacgcgc ccatctggta
     4501 ctcgctccgc agcgcctcgc gcgcggccgc cttggcgttc gggagcttgg ccatgtccag
     4561 cggagcgaag agcccgtccg agacgtagag ccgccccacg tggctcgtgg tgaacgtgat
     4621 gtccaccagc gggctgccct tggccacccg cgccttggcg aggcggtcga tggtgccgcc
     4681 cacctcgaac tcgaccggca tgccggtctt cgcggtgaaa gccttcccga tgacgcggtc
     4741 gacggtgtcc ttccagttgc cgccccagac gctcaccacc agcgagttct gggcatgggc
     4801 gggagcgacg ggcccgaagg gcaggcacag cgcgagcagg gtcacgacgg cgaggcgcgc
     4861 gagcatatgc atctctccta tggagtgcgg gtgggaggcg actcgagcgc ccgagcggcc
     4921 gcctcgatgg cctcgacgat gaagtggtag ccggtgcagc ggcagaggtg cccggccagc
     4981 atctcgcgga tctcctcgtg cgacggcgcg ggccgctcgc ggagaaacgc gcaggcgctc
     5041 atgatgatac cgggagtgca gtagccgcat tgcagcccgt gcttggccca gaacgcctcc
     5101 tggatcgggt ggagcccgtc ggcgggggcg acgccctcga tggtcgagac gcgctggccg
     5161 tcggcctgta ccgccagcat caggcaggcg cgcaccggct ctccgtcgag caggaccgtg
     5221 cacgccccgc acaccccgtg ctcgcagccc acgtgggtgc cggtgagatc gaggtcttcc
     5281 ctgaggaaat cggccagggt cgcgcgcacc tcgaccgcgc gctcgtgtgc ctggccgttc
     5341 acggtcaccc ggatcgcctt gttcacgaca ggccccctcc gcaccgcgcg agcgccgcgg
     5401 tcagcgtgcg ctcggccagg gtggccgcga cccgccgccg gtaggacgcg gtcgcgtgga
     5461 tgtccccgtc gggcgagagc cccgccgccg ccgcccgcgc ggcttcgccg atcagcgccg
     5521 gcgtcggcgc gcggccttcc agcgcccgct cggcggccgc gccccggacc ggcgtcccgc
     5581 cgacgccgaa gaaggccagc cgcgcccgcg ccaccgtgcc gtcggccgcg cacgtgagca
     5641 cggccaccgc gcccaccagg gcgaagtcgc cgtgacggcg cgacacctcc gcgaaacccc
     5701 agcccgagcc ggccgccggc agcgtgaagc gcaccgcggt ggccagctcg tggccgtcga
     5761 gcgcggtggt cagcggggca cggtagaacc ggtcggccgg gatcatgcgc tcgccccggg
     5821 gcccgcgcgc gaccaccacg ccctcgaggc agaggagcac cgcgggcagc tccgaggccg
     5881 gatccgcgag gacgatactg cccaccaccg tgccgcgatt gcggatcggc gggtgcgcga
     5941 ccgcgtgcag cacctcggcg aagagtggcg agcggccggc cgcccagcgc tcgagcgcgc
     6001 gctggcggac gagcgcgccg atctcgagga tgcccgaggc ctcgcggatc ccatcgagcc
     6061 cccgcagccg gttgatgtcc accagcacgg acggccgggc cagccgcagc gccagcatcg
     6121 ggatcaggct ctggccgccg gccaggatct tggcgtcgtc gcccgcggtg gccagcgcgc
     6181 cgatggcgtc gtcgagggtg gtcggcgcca cgtagtcgaa cggagcgggc ttcatgcggg
     6241 cctcccgcct cgtgccgccg cctgatcgag cagcccgagc accaccgacg ggccgagcgg
     6301 ggtgcgggtc acccgcacgc cgaacggcgc gagcgcgtcc tccaccgcgt tggcgatggc
     6361 ggcgggcggc gagatcgccc cgccctcgcc gatgcccttc aggccgagcg ggttccgcgg
     6421 cgatggatac tcgaggtgca cggtctcgat gggcggcagc tccatcgcgg tcggcaccag
     6481 gtagtccatg aacgtcccgg tgaggagctg cgcctgctcg tcgtagacca tctcttccag
     6541 aagcccgccg cccacgccct gcgcgacgcc gccgtggatc tggccttcga cgatcaccgg
     6601 attgatgatc gtcccgcagt cgtgcgccac caggtaccgg agcagcttca ccgcgccggt
     6661 gccggtgtcc acgtccacca ccgcgacgtg cgtggcgctc gtgtaggtca cggtcggctg
     6721 gtgatggtag acggtcgcct cgaagtcggc cggggccacg cccggcttcg cgaacgtggg
     6781 gatcgcggcc tggatcacct tcgagatcgg gatcgcggag gccggggcgc cgcgtaccga
     6841 gaccatgccg tccgccagct cgacgtcggc gggcgcggct tcgaggagcg cggccgccgc
     6901 cgcgaccagc ttgtccttca cgcggcccgc cgcctcgtgg atcgagctgc ccgcgttgac
     6961 cgcgctgcgc gaggcgaagg tgccgacccc gaacgggatc gcggcggtgt cgccgccgac
     7021 gatggtcacc gcctcgagcg gaatgccgag ggcgtcggcc gcgatctggg cgaacgaggt
     7081 ctcgtgcccc tgcccctggc agcaggcgcc ggtcgcgacc accgcgtggc ccgagacgtc
     7141 gaggcggacc gtcgcgcctt catagggccc gatcgcggtg ccctccacgt agctcgagat
     7201 cccgatgccg cgatggaccc cccgctcgcg cagccgcgcc tgctcggcgc gcagggcatc
     7261 gtagccgacg gcccgcaggg ccgcctcgag cccggccttg aagtcgccgc tgtcgtagac
     7321 cagcgggttg ccgtcgcgat acgggatccg cagctcgtag ggcagatcgg ccgcggacag
     7381 gaagttgcgc cggcgcagct cggccggatc catggcgagc tcccgcgcca ggcagtccac
     7441 gatgcggtcc atggcgaaca ccgtctcggg gcggcccgcg ccgcgatagg gcgcgttcgg
     7501 cgtcttggtg gtcacgacgc cgaggcactc gacgtcgagg ttcgccacgc gatgcgggcc
     7561 cagcaggtgg gccaccgtgt tgtagggcag cacgataccc caggagttgt aggcgcccag
     7621 gtccacccag atccgatcgc gcaccgccag catcgtcccg tcccgccggg ccgcgatctc
     7681 gatatcgtgc acctgggcgc gcgcgtgggc cgagcccatc atgtgctcac ggcgatcctc
     7741 ggtccacttc accggccggc gcgccaggat ggccgcgg
//
LOCUS       DASUFA010000451         6145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_560138, whole genome shotgun sequence.
ACCESSION   DASUFA010000451 DASUFA010000000
VERSION     DASUFA010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560138"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      30..130
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            173..373
                     /gene="thiS"
                     /locus_tag="VFX87_16340"
     CDS             173..373
                     /gene="thiS"
                     /locus_tag="VFX87_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX4996551.1"
                     /translation="MTVTVNGKPMELPEGLSVEDLLARLKVRREFTAVALNREVTQRA
                     RYAETRLQDGDKLEIVHPMGGG"
     gene            385..1167
                     /locus_tag="VFX87_16345"
     CDS             385..1167
                     /locus_tag="VFX87_16345"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX4996552.1"
                     /translation="MYDTSLWLGGKEFKSRLIVGTGKYPSFENMREAIEASGAEIVTV
                     AVRRISLPGQGESLLDFVDPKKYTLLPNTAGCYTADEAVRTCYLAREAGLGTLVKLEV
                     IGDSRTLFPDVIGLLEATRTLSREGFTVLPYTNDDPVMAKKLEDAGAAAVMPLGAPIG
                     SGLGIRNPYNIKLILEAVTVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIASAKDPV
                     AMATAMRLAVEAGRLAFKAGRIGKKLYATASSPLEGVPEWTS"
     gene            1155..1781
                     /gene="thiE"
                     /locus_tag="VFX87_16350"
     CDS             1155..1781
                     /gene="thiE"
                     /locus_tag="VFX87_16350"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX4996553.1"
                     /translation="MDQLGFSLYLITDRAAAPRPAADVVEECLAAGLRAVQLREKDLE
                     ARDLLALADTLREATQRHGARLIVNDRADVALAAGADGVQRTHASLPVSVLREITPPG
                     FLVGASVHSEAEARDAAAQGADFIVFGPVYDTASKRRYGPPQGLAALGAVTRAVDRPV
                     LAVGGLTPERVAEVLAAGAAGVAVIGAISAAPRPADATKAFLDALGRA"
     gene            1781..2893
                     /gene="thiO"
                     /locus_tag="VFX87_16355"
     CDS             1781..2893
                     /gene="thiO"
                     /locus_tag="VFX87_16355"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX4996554.1"
                     /translation="MAAPSVAVIGGGIIGCATAADLAKRGCRVTLFERASPGAEASSA
                     AAGLLAPLGPSPEPGPFHRLAIESWRLYPRVASELRDLTGVDVEHMTAGTLYPLHSPR
                     EIEAARLRRTWPLAGEFGIEVVEGSDLRALEPALSKDVSAALFIRGDHWVNNQRLVTA
                     YAAAAAALGVMVRTGAEVSRILIEGGRARGVHVDGEAIHTDAVLLAAGAWSGALATAI
                     GLRLPVAPVRGQMLAVSNVPPLITRAMHGDDIYVVPRPSGEVLIGATVEHAGFLRAVT
                     PDGLGSLIAAAVALVPEIGRRPVTRSWCGFRPWVPDGQPVLGPWPGVAGLFTATAHYR
                     NGILMAPITAALLTRCIVDGDTPASITPFLPSRFAP"
     gene            3039..4373
                     /locus_tag="VFX87_16360"
     CDS             3039..4373
                     /locus_tag="VFX87_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4996555.1"
                     /translation="MTTKKELKDAVCKAIDRNSERIIGLGERIRRQPELGFKEFKTAR
                     LAEDTLGELGLKPRSGLALTGVRAEAAGAKDGPTFALLGELDGLVVAGHPIADPETGA
                     AHACGHNAQMAGLLGAAMGLCEARAFEHLAGRVVFFAVPAEEYGDVEWRVQQARAGRL
                     EFLGGKPELLRLGHFDDVDLAMMIHTTSQPDMKKAGVAASNNGCLVKTVRYIGRASHA
                     GGAPHMGINALYAANIGLAAINALRETFRDEDSIRVHPIITHGGSQVNVIPGEVRLET
                     YVRGKTVEAILDANRRVDRALRAGALALGAQVEIETLPGYLPLFNHMGMAQQFKANAA
                     ALLGADQVTESGHRSGSTDMGDISHVMPTLHPYMGGATGGGHGADYAIADPKLAYVEP
                     AKQLALMAVDLLWDDAGAAREILRAWKPRMTKEEYLTFQRGTARTELYDGSK"
     gene            4444..5250
                     /locus_tag="VFX87_16365"
     CDS             4444..5250
                     /locus_tag="VFX87_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX4996556.1"
                     /translation="MYGKSPVEFPRRIVCLAAEHVEICYALGAGDRVVGVPGTARRPA
                     EAAAKPHVGGFTTFRVDRILELQPDLILAFSDLQADISAELIRAGLAVFCTNPRSVEE
                     VMQTILTVGGLLGAEPAARALVLDMRDEITQVREFSRVWPDRPRVYFEEWHDPLIAGI
                     RWVSELIELAGGRDVFADLRDRKSARERVVDPAEVVRRDPQLIVASWCGKPADLAAIA
                     ARPGWDRISAVRGGRIHEMDGADILSPGPSLLIGLRRLHELVQEALDARD"
     gene            5420..>6145
                     /locus_tag="VFX87_16370"
     CDS             5420..>6145
                     /locus_tag="VFX87_16370"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX4996557.1"
                     /translation="MLARPERFFADRFGLNAGTMERLLGSTLSGRVDHADLFLEYRIS
                     EELVLEEGSVKKAARHVSQGAGLRAQSGERTGYAHTDDLALPNLEEAARQARAIADRS
                     ATSAVVAVGRDGPPHDLYTLAEPPTSTDLDRKLKLLGRVDAEARGLDPRVRQVIATLG
                     SEEVVVMIATPSGWTVGDVRPLTRLNITVIVEDGAKREIGSYGGGGRVAFDFFLEQDR
                     WKRFTGEAVRQALMKLDAVDAPAG"
BASE COUNT          908 a         2195 c         2207 g          835 t
ORIGIN      
        1 gcgccagctc gagggtgtat actccgaatc gccgacgcgg ggggaggtct cctgagaacc
       61 gggtgcggcc cgggacccct cgaacctgca ctgggtaatt ccagcgaagg gaacgcgacg
      121 cggcgcacga cactggaccg caccctttcc tggaaaggtg tgcggttttt tcatgactgt
      181 caccgtcaac ggcaagccga tggagctgcc cgaggggctg agcgtcgagg atctcctcgc
      241 gcgactcaag gtccgccgcg agttcaccgc ggtggccctg aaccgcgagg tcacccaacg
      301 cgcccgctac gcggaaacca ggctccagga cggcgacaag ctcgagatcg tccaccccat
      361 gggcgggggg taagggggtt caccatgtac gacacgtcac tgtggctggg cggaaaagag
      421 ttcaagtcgc gtctgatcgt cgggaccgga aagtacccgt cgttcgagaa catgcgcgag
      481 gccatcgagg cctccggcgc cgagatcgtc acggtggcgg tgcgccggat cagcctgccg
      541 gggcaagggg aatccctcct cgacttcgtg gaccccaaga agtacacgct gctgccaaac
      601 accgcgggct gttacaccgc cgacgaggcc gtccggacct gctacctggc ccgtgaggcg
      661 ggcctgggga ccctggtcaa gctcgaggtc atcggcgact cgcgcaccct gttccccgac
      721 gtgatcggcc tgctcgaggc gacccgcacg ctgtcccgcg agggcttcac ggtgctgccg
      781 tataccaacg acgacccggt tatggccaag aagctcgagg acgccggcgc ggccgcggtc
      841 atgccgctgg gtgccccgat cggctccggg ctcggcatcc gcaacccgta caacatcaag
      901 ctgatcctgg aggccgtcac ggtccccgtg ctcgtggacg cgggagtggg cacggcgtcg
      961 gacgcggcca tcgccatgga gctcggctgc gacggggtgc tgatgaacac cgccatcgcc
     1021 tctgccaagg acccggtggc catggccacc gccatgcgcc tggccgtcga ggccggccgc
     1081 ctcgccttca aggccgggcg aatcggcaag aagctctacg ccaccgcgtc ctccccgctg
     1141 gagggcgtgc ccgagtggac cagctaggct tttccctcta cctgatcacc gaccgcgcag
     1201 cggcgccgcg gccggccgcc gacgtggtgg aggagtgcct ggccgcgggc ctccgcgccg
     1261 tgcagctccg cgagaaggat ctcgaggcgc gcgacctgct cgcgctggcc gacacgctcc
     1321 gcgaggccac ccagcgtcac ggcgcgcggc tcatcgtcaa cgaccgggcc gacgtcgcgc
     1381 tggccgccgg ggccgacggc gtccagcgca cgcacgcctc gctgccggtg tcggtgctgc
     1441 gcgagatcac accgcccggg ttcctcgtgg gcgcttccgt ccattcggag gccgaggcac
     1501 gcgatgccgc cgcgcagggc gcggacttca tcgtcttcgg gccggtctac gacaccgcgt
     1561 ccaagcggcg ctacggcccg ccacagggtc tggccgcgct cggggccgtc acccgggcgg
     1621 tggaccgccc ggtcctggcg gtgggcggcc tcaccccgga gcgcgtcgcg gaggtgctcg
     1681 cggccggcgc ggcgggggtc gcggtgatcg gcgcgatctc cgcggccccg cgacccgccg
     1741 acgccacgaa ggcgtttctc gacgcgctgg ggcgggcgtg atggccgcgc cctccgtggc
     1801 ggtgatcggc ggcggcatca tcggctgcgc gaccgcggcg gatctcgcca agcgcggctg
     1861 ccgcgtcacg ctcttcgagc gcgcgagccc gggcgccgag gcctcgagcg cggcggcggg
     1921 cctgctcgcc ccgctcggcc cgtcgcccga gcccggcccc ttccaccggc tggcgatcga
     1981 gagctggcgc ctctacccgc gcgtggcctc cgagctccgg gatctgaccg gcgtggacgt
     2041 cgagcacatg accgcgggca cgctctaccc gctccactcg cctcgggaga tcgaggcggc
     2101 ccgtctccgc cggacctggc cgctggccgg ggagttcggt atcgaggtgg tggaggggag
     2161 cgacctgcgt gcgctcgagc ccgcgctctc caaggacgtg agcgcggccc tgttcatccg
     2221 gggcgatcac tgggtgaaca accagcggct ggtgaccgcc tacgcggcgg ccgccgcggc
     2281 tctgggtgtc atggtgcgca cgggcgccga ggtgagccgc atcctgatcg agggcgggcg
     2341 tgcccgcggc gtccatgtgg acggcgaggc gatccacacc gacgcggtgc tgctggcggc
     2401 cggcgcctgg tcgggagcgc tcgccaccgc catcggcctc cggctgccgg tggccccggt
     2461 gcgcggccag atgctggcgg tgagcaatgt tccgccgctg atcacgcgcg cgatgcacgg
     2521 cgacgacatc tacgtggtgc cgcggccctc gggtgaagtg ctgatcgggg cgaccgtgga
     2581 gcacgcgggc ttcctccgcg cggtcacgcc ggacggcctc ggctcgctca tcgcggccgc
     2641 cgtggctctg gtccccgaga tcggccgccg gccggtcacc cgctcctggt gcggcttccg
     2701 gccctgggtc cccgacgggc agccggtgct ggggccctgg cccggcgtgg ccggcctctt
     2761 cacggccacc gcgcactacc gcaacggcat cctcatggcg cccatcaccg cggcgctgct
     2821 gacccgctgt atcgtggacg gcgacacccc ggcgtcgatc accccctttc tccccagccg
     2881 cttcgcaccg taacccgacc cggtcgccac gacgccccga tgagccccgc agggggcgaa
     2941 gaggccacat cgcagaccgg agtgacccaa cgctgacgac ttggatagag ccccgcaggg
     3001 ggcgaagagg ccaaacctca acgcggagtg aaagaccaat gacgacgaag aaagagctca
     3061 aggacgcggt gtgcaaggcc atcgaccgga acagcgagcg catcatcggc ctgggcgagc
     3121 gcatccgccg ccagcccgag ctcggcttca aggagttcaa gaccgcgcgg ctggccgagg
     3181 acactctcgg cgagctcggg ctcaagccgc ggagcggcct cgcgctcacc ggcgtgcggg
     3241 cggaggccgc aggcgcgaag gacggcccca ccttcgcgct cctgggcgag ctggacggcc
     3301 tggtggtggc ggggcacccg atcgccgacc ccgagacggg cgccgcgcac gcctgcggcc
     3361 acaatgccca gatggcgggg ctcctcggcg ccgccatggg cctctgcgag gcgcgcgcct
     3421 tcgagcacct ggccggccgc gtggtgttct tcgcggtgcc cgcggaggaa tacggcgacg
     3481 tggagtggcg cgtgcagcag gcgcgcgcgg ggcgcctcga gttcctgggc ggcaagcccg
     3541 agctgctgcg cctcggccac ttcgacgacg tggacctcgc catgatgatc cacaccacgt
     3601 cccagcccga catgaagaag gcgggagtgg ccgcctccaa caacggctgc ctcgtgaaga
     3661 cggtgcgcta catcggccgc gcctcccacg cgggcggcgc cccgcacatg ggaatcaacg
     3721 cgctctacgc cgccaacatc ggcctcgccg cgatcaacgc cctgcgggag acgttccgcg
     3781 acgaggactc catccgcgtg cacccgatca tcacccacgg cgggagccag gtgaacgtga
     3841 tcccgggcga ggtgcgcctc gagacctacg tccgcggcaa gacggtcgaa gcgatcctcg
     3901 acgccaaccg ccgggtggac cgcgcgctcc gcgccggcgc gctggccctg ggtgcccagg
     3961 tggagatcga gaccctgccc ggctacctgc cgctcttcaa ccacatgggc atggcgcagc
     4021 agttcaaggc caacgcggcg gcgctgctgg gcgcggacca ggtgaccgag tcgggccacc
     4081 gcagcgggtc gaccgacatg ggcgatatct cccacgtgat gccgacgctt catccgtaca
     4141 tgggcggggc caccggcggc gggcacgggg ccgactacgc gatcgcggat ccgaagctgg
     4201 cctacgtgga gccggccaag cagctcgcgc tgatggcggt ggacctgctc tgggacgacg
     4261 cgggcgccgc gcgcgagatc ctccgggcct ggaagccgcg catgaccaag gaggagtacc
     4321 tcaccttcca gcgcggcacc gccaggaccg agctctacga cggtagcaag taaatctatc
     4381 ctcatccccc tctctcgtca gggcagaggg aagggtgagg ggtggaaggt gaggggaggc
     4441 cccatgtacg gcaagtcccc ggtcgagttc ccgcgccgca tcgtctgcct ggccgccgag
     4501 cacgtcgaga tctgctacgc cctcggcgcg ggcgaccggg tggtgggcgt gcccggcacc
     4561 gcgcggcggc ccgcggaggc cgcggccaag ccccacgtcg gcggcttcac gacgtttcgc
     4621 gttgaccgga tcctcgagct ccagccggac ctgatcctcg cgttctccga tctgcaggcc
     4681 gacatcagcg cggagctgat ccgggcgggg ctggcggtgt tctgcaccaa cccgcgctcg
     4741 gttgaggaag tcatgcagac gatcctcacc gtgggcggcc tcctcggggc cgagcccgcg
     4801 gcccgcgccc tggtcctcga catgcgcgac gagatcaccc aggtgcggga gttctcgcgc
     4861 gtgtggcccg accggccgcg ggtctatttc gaggagtggc acgacccgct catcgcgggc
     4921 atccgctggg tctcggagct gatcgagctg gcgggcgggc gtgatgtgtt cgccgatctc
     4981 cgcgaccgga agagcgcgcg agagcgggtg gtcgatcccg ccgaggtcgt ccggcgggac
     5041 ccgcagttga tcgtcgcgtc ctggtgcggc aagccggccg acctcgccgc gatcgcggca
     5101 cgcccgggct gggaccggat cagcgcggtc cgcggcggcc gcatccacga gatggacggc
     5161 gccgacatcc tgagcccggg cccgtcgctg ctgatcgggc tgagacgcct gcacgagctg
     5221 gtgcaggaag ccctggacgc gcgcgactga tctcgactcg cgcctcagtc ccgcgcgggc
     5281 aagacgtgga cacatctgag ataatcgaaa cagtcgtatc gggcgctcga acatgtactg
     5341 aagcaggcac taaagatggg ggcccctccc ggagccgatg ctaggatgaa gcccctttga
     5401 gacctccaag gagacggaaa tgctagcgcg ccccgagcgc tttttcgcgg accggttcgg
     5461 cctcaacgcg gggaccatgg agcggctgct cggctcgacg ctatccggcc gcgtggacca
     5521 cgcggatctc ttcctcgagt accggatcag cgaggagctg gtgctggagg agggctcggt
     5581 gaagaaggcg gcccggcacg tgagccaggg ggcgggcctc cgggcgcagt ccggggagcg
     5641 caccggctac gcgcacaccg acgatctggc cctgcccaat ctcgaggaag ccgcgcgcca
     5701 ggcgcgcgcg atcgccgacc gctcggcgac ctcggcggtg gtggcggtgg gccgcgacgg
     5761 cccgccgcac gatctctaca ccctggccga gccccccacc tcgaccgatc tggaccgcaa
     5821 gctcaagctc ctgggccggg tggacgcgga agcccgcggc ctcgaccccc gcgtgcgcca
     5881 ggtcatcgcc accctcggta gcgaggaagt ggtggtcatg atcgcgacgc cgtcgggctg
     5941 gacggtcggc gacgtgcgcc cgctcacgcg gctcaacatc acggtgatcg tggaagacgg
     6001 agccaagcgc gagatcggga gctacggcgg aggcgggcgc gtggccttcg acttctttct
     6061 cgagcaggat cgctggaagc gcttcaccgg cgaggcggtg cgccaggccc tcatgaagct
     6121 cgacgcggtg gacgcgcccg cgggc
//
LOCUS       DASUFA010000452        10425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_566043, whole genome shotgun sequence.
ACCESSION   DASUFA010000452 DASUFA010000000
VERSION     DASUFA010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566043"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFX87_16375"
     CDS             <1..807
                     /locus_tag="VFX87_16375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996558.1"
                     /translation="MAVGLTLIFGVMRVVNFAHGDMMVWGMYLAWILSTRAGVDPYVG
                     FIVCAAALFALGWATQRLLVDRIVEAPHEMQILLMLGVALVLENVALVAFGPDPQRVR
                     SPLRAATFWLGPVFVDVARLVTFGLAMVLTLALYLFLTRTDLGRTMRAAADNAYGALV
                     IGTDVRRVYAAAFGIGAACVGAAGALVSPILPFQPAGGLSLSVASFNIVIIGGMGSLP
                     GAFLGGLLVSVAESLGAVFLSPSMKELVSFSLLIVILLFRPAGLFGKTAA"
     gene            804..1754
                     /locus_tag="VFX87_16380"
     CDS             804..1754
                     /locus_tag="VFX87_16380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4996559.1"
                     /translation="MKWLAAAALLVLLLLPAVLNSYAVTVFIFIFFYAFLGQAWNIVG
                     GYAGQLSAGHAAFVGIGAYTSAVLSVQAGLTPWIGMLVGGLLSAGLGTVVGYLGFRFG
                     LRGFYFVLLTVAFGEVCRIVALNTDAVGGALGYYITFTGNPWQFQFQDNRVYYYVALG
                     LMLIATGIVAAIERRRFGAYLIAIREDESACEALGVNTFRYKMLAMIVSSFLTGVGGT
                     FYAFYLFSLQPGAVFGIPLSVEIIIRPIVGGAGTMMGPILGSFILSPLAEASRIYFGQ
                     GGWAGAHLIAYGLLLIAVVLFLPQGAYPALRRLWLRLAPR"
     gene            1751..2524
                     /locus_tag="VFX87_16385"
     CDS             1751..2524
                     /locus_tag="VFX87_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996560.1"
                     /translation="MSACAKRPGASARPPLLEVSAISKRFGGLVAVHDLSLEMARGEV
                     LGLIGPNGAGKTTAFNLLSGFLTPDEGTVHFDGHPLVGLKPHAICALGLARTFQIVRP
                     FPHLSVLDNVRVGALARRPRMAEAREKARAVIDRVGLGAKAGHAASGLTLAERKRLEL
                     ARALATEPTLLLLDEVMAGLNPTEIAAIVSLIQRINADGVSILLIEHNMRAVMTLSHR
                     IAVLNFGEKIVEGPPATVANHPRVIEAYLGEEYVHTPAR"
     gene            2502..3215
                     /locus_tag="VFX87_16390"
     CDS             2502..3215
                     /locus_tag="VFX87_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4996561.1"
                     /translation="MSTLLRVDGIEVGYGDVTAVGDVSLEVREGEAVALIGANGAGKT
                     TTLRAVSGLLPLRRGRIEFEGQRLDGLTSAQIVARGIAHVPEGRQLFPTMTVLENLEL
                     GARTGAARARRAESLERVFDLFPRLRERRGQLAGTLSGGEQQMCAIGRGLMACPRLLM
                     LDEPSLGLAPVMVKAIFEDLGRINAQGLTILVVEQNVLRSLHLAHRGYVLENGRVTRS
                     GAGPELLASGHIKQAYLGL"
     gene            3264..>10425
                     /locus_tag="VFX87_16395"
     CDS             3264..>10425
                     /locus_tag="VFX87_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4996562.1"
                     /translation="MRRFIRGFLVPFLLLAIPLGILAGLGFIAAHRQLVRTRDAAVAR
                     TVDAVSALVADYQESLRRETILLARDPAVVEGAAKGDWAILARGASPRVLAITRDGLA
                     DFITIRDSRGTPLVQVPASPPPSLPGAPAATEPVLTLRLAGGQPYFLVTAPVQSPAGR
                     EPPGGPGSVVAGRRVEGLGALLDRLPARPAVVFVAGDRALAASRSDLPTSGWSRSATV
                     GATDVGDEPFAVRRLVEPVVTSPDGGLWVVLSVREFMRAEHRLLLEFLALLGAGAVVL
                     AGVVLAFLPAGGRPRRPAGSGPAPVSPRVALERRNRELEALNAVFATMSRGSDLATTA
                     GETLEVVRALARMDVGVVYRLDQDGNQLVLEGQSGVDPRYLDRSRNRPVEGSRIGDAA
                     RTGQIIVTHLDASPPSEEQIREMAAERAHRTQLALPIPVEHRTWGVMVLVSKERREFL
                     PEELTILSAVAQQVGLAVERAQLRDTAAVRLSRLEAQRMIERHISEQLDTEELLVVIA
                     RSAQRLIGGTFSALYLLEGDTLRPRAWSDVPDWIRDLRFKIGTGVAGAALAAGRGALA
                     NDYPSSPGAMAEFIPFTSRLLAAPLMAGDRPLGVMTAGRGPGGPPFTEEDLSTLTDFA
                     TQAAVALEHARLFDEATRNAAQYQALLEVSSAVSSTLDVDRVLDLVLQRCQALLGVAA
                     VGVMRIDRETGVVAYERGRGLSGEFTTSLRMRLGEGTTGRAIEERVPVWSEDVLNDAA
                     LAISPAARELIRREGYRAVLSVPLLTKGDAHGAIAAYWWEPHTPSAAEISIMTALAGQ
                     AATALDNARLFAQERDRKASLSSLLEINKKIGALVAPESLLTSIAEEAARLLDVDNAG
                     FRLLDGDDLVVAGVAGAAAQSMLKPRIKVGESLTGQVLATGQALMCEVEASGLLADHL
                     AADQQLGYTHYLGVPLLVGERAIGVLAFRGRRAFTAREQELGETFAGQAAIAIDHSRL
                     YREASDQAERMRVVAEMGRVLVSTLDEARVLEIVSTQAHDSLGRLDIAIWLQEGHGGP
                     LRLVTGQGRFSSPLAGRAQPLDPDEGVVGLALTERAPVWTADVLTDSRIRLRPESRRW
                     IEEIGGRSLLAVPLIREHLLGALVVYRPVGQVFSTREVEYLSAFANQVAVALENARLY
                     RALDVRAARLRSLARLAHIVSSSLDMDEVLRAIAEAAGELIAVPLASFWVANEPAGTL
                     EFRTSSDAMADDFPSKSLPYGEGGVGWVALHRQTLEIEEIAQDSRFKFTEWAVSHGLT
                     SLLAVPIVFQGTLLGVLSLNGRQAIRLSPDDQQLLESFVAQAGVAIRNAGLYGETRGR
                     LEESRALLEVAEILNSTLDSRRLLREVTMKIAQVCRVDRCSIQRWIDGRVVPLMSQYA
                     DGHPDPETWDRFRRMAASLPMRPPLHARTVDTRRPVIVPDAASSELVPAEWVELFRLK
                     STMTVPLIRQDEVIGVMALDHTERATSFEPWQVDLAMAIAGQLALSLANTQLYTQVQE
                     RLRETTALLAVGRALSQPEPTAHVMRTVAREVAQAFGADMVGVYSLDPKHEALVPTAG
                     YHVPKHLLEHFLTQPFTLARFPALTQVWREGRAAWSSDANADPRFDRGVLEGIDAHSV
                     LFAPTTVRGEPVGALFLVWWQTGREFSESEVRLLEGVAAQVGLGMENAELARQTELKL
                     QETETLLAVGQTLASTLDVDTLTRQFLRHVAHGLGADSTGMWLLGDDGEWMAPLAGYH
                     VPADRLDALRGLRLSIRDHEFYREAAEQRRSVISTDGAHDPRIPEEIRRRAPVRTHLF
                     VPIFAQEQMIGGFSANWWDKVRELSDAERRLMEAVASQAGVALQNARLFQDNQRRVRE
                     LSVLHELSRSVTGQLDQAGILDTLQQQVPRVLDVRHMSAMLLDEEHERLDVVLRVRDG
                     RRCDEDEPRSYPLDQAGLSSLVFNSGRPIRTADYAGECRRQGVTPVASAMDMPHAIVV
                     PMTAGDRVIGLLSLRSPDRAFTDADERLLVNIAQLVALMLRSARLYEERTRALGELGA
                     AQDQLVRTEKLRALGEMASGVAHDFNNLLASILGRAQLLLERIQDVKLRQWLKVIERA
                     AMDGARTVRRLQDFTGIRRDQPAVDVDLNQVLQQVLETTESIWRQDRGRSGGAEIQVE
                     TALAAELPPVAGDPAELREAFTNLVLNAVDAMPKGGTLTLRTRAAEGQVEVEVRDTGT
                     GIPEHIREKIFDPFFTTKGPKGTGLGLSMAYGILQRHNGRITVESEAGHGTTFRLLFP
                     AVVSGSVADAVPPLTPAPGALVSLHCLVVDDEQEVGEVVADILTTAGHTAVTVGNGKE
                     GVDRLGVERFDVVFTDLAMPGMTGWQVARAVKDRAPGVAVVMMSGFGVEVAPEDLHTH
                     GVDLVLAKPLQIHDVLRAVEAIR"
BASE COUNT         1329 a         3746 c         3761 g         1589 t
ORIGIN      
        1 atggccgtgg ggctcaccct gatcttcggg gtgatgcgcg tcgtcaactt cgcccacggc
       61 gacatgatgg tctggggcat gtacctggcc tggatcctct cgacccgcgc aggggtggat
      121 ccctacgtcg ggttcatcgt ctgcgcggcc gctctcttcg cgctcggctg ggccacccag
      181 cgcctgctcg tggaccggat cgtcgaggcc ccgcacgaga tgcagatcct cctcatgctg
      241 ggcgtcgccc tcgtgctcga gaacgtggcc ctggtggcct tcggccccga tccccagcgg
      301 gtgcgctcgc cgctgcgggc ggccaccttc tggctcggcc cggtgttcgt ggacgtggcg
      361 cggctcgtca ccttcgggct ggccatggtg ctcaccttgg ccctctatct gttcctcacg
      421 cgcaccgatc tggggcgcac catgcgcgcg gccgccgaca acgcctacgg ggcgctcgtg
      481 atcggcaccg acgtgcggcg cgtctacgcg gccgccttcg gcatcggggc cgcctgcgtg
      541 ggggcggcgg gggcgctcgt ctcgccgatc ctgccgttcc agcccgcggg cgggctctcg
      601 ctctcggtgg cctcgttcaa catcgtgatc atcggcggca tgggcagcct gcccggcgcg
      661 ttcctgggcg gcctgctcgt ctcggtcgcc gaatcgctgg gcgcggtgtt cctgagcccg
      721 tccatgaagg agctggtgag cttctcgctg ctcatcgtga tcctgctctt ccgcccggcc
      781 gggctcttcg ggaagaccgc cgcgtgaagt ggctcgccgc cgccgcgctg ctcgtcctcc
      841 tgctgctgcc cgccgtgctc aactcctacg cggtcaccgt gttcatcttc atcttcttct
      901 acgccttcct cgggcaggcc tggaacatcg tggggggcta cgccgggcag ctctcggcgg
      961 gccacgcggc cttcgtgggg atcggcgcct acacctccgc ggtcctctcg gtgcaggccg
     1021 ggctgacccc gtggatcggc atgctcgtgg gcggcctgct ctcggccggc ctcggcaccg
     1081 tggtcggcta tctcggcttc cgcttcggcc tgcgcggctt ctacttcgtg ctgctcacgg
     1141 tggccttcgg ggaggtctgc cgcatcgtgg cgctcaacac cgacgcggtg ggcggggccc
     1201 tcggctacta catcaccttc acgggcaacc cgtggcagtt ccagttccag gacaaccgcg
     1261 tctactacta cgtggcgctc gggctgatgc tgatcgccac cgggatcgtg gcggccatcg
     1321 agcgccgccg cttcggcgcc tacctgatcg cgatccggga ggacgagagc gcctgcgaag
     1381 ccctcggggt gaacaccttc cgctacaaga tgctggcgat gatcgtctcg tcgttcctca
     1441 ccggcgtggg cgggacgttc tacgccttct acctcttctc gctccagccg ggcgcggtct
     1501 tcggcatccc gctttccgtc gagatcatca tccggcccat cgtgggcggc gccgggacga
     1561 tgatgggccc gatcctcggc tcgttcatcc tgagcccgct cgccgaggcc tcgcggatct
     1621 acttcgggca gggaggctgg gcgggcgcgc acctgatcgc ctacggcctc ctgctgatcg
     1681 cggtggtgct cttcctgccg cagggcgcct accccgcctt gcgccgcctc tggctgaggc
     1741 tggcgccccg atgagcgctt gcgcgaaaag gccaggagcg tccgcccgcc caccgctgct
     1801 cgaggtctcc gcgatctcca agcgcttcgg cgggctggtg gcggtgcacg atctctcgct
     1861 cgagatggcg cggggggagg tgctcggcct catcggcccc aacggcgccg gcaagaccac
     1921 cgcgttcaac ctgctctcgg gttttctcac gcccgacgag ggcacggttc acttcgacgg
     1981 ccatccgctc gtcgggctca agccgcacgc catctgcgcc ctcggcctcg cccgcacctt
     2041 ccagatcgtc cgcccgtttc cccacctctc cgtgctcgac aacgtgcggg taggcgcgct
     2101 ggcgcggcgg ccccggatgg cggaggcgcg ggagaaggcg cgggcggtga tcgaccgggt
     2161 ggggctcggg gccaaggccg gccacgcggc gtccgggctc acactggccg agcgcaagcg
     2221 gctcgaactg gcgcgggccc tggccaccga gcccaccttg ctcctgctcg acgaggtcat
     2281 ggccgggctc aaccccaccg agatcgcggc gatcgtgagc ctcatccagc gtatcaacgc
     2341 ggacggcgta tccatcctgt tgatcgagca caacatgcgg gccgtgatga cgctctccca
     2401 ccgcatcgcg gtcctcaact tcggggagaa gatcgtggag ggcccaccgg ccacggtggc
     2461 caaccaccca cgcgtgatcg aggcctacct gggcgaggag tatgtccaca ctcctgcgcg
     2521 ttgacggcat cgaggtcggc tacggcgacg tgaccgcggt cggcgacgtc tcgctcgagg
     2581 tgcgggaggg cgaagcggtg gcgctgatcg gcgccaacgg cgcgggcaag acgaccacgc
     2641 tgcgcgcggt gtcgggcctg ctgccgctcc gccgcgggcg catcgagttc gagggtcagc
     2701 ggctcgacgg gctcacgtcc gcccagatcg tggcccgcgg gatcgcccac gtgccggaag
     2761 ggcgccagct cttcccgacg atgacggtcc tggagaacct cgagctgggg gcccgcaccg
     2821 gagcggctcg ggcgcggcgg gccgagagcc tcgagcgggt cttcgacctg ttcccccgcc
     2881 ttcgcgagcg gcgcgggcag ctggcgggga ccctctcggg tggcgagcag cagatgtgcg
     2941 ccatcggtcg cgggctcatg gcctgtcccc ggctgctcat gctggacgag ccgagcctgg
     3001 ggctggcccc ggtcatggtg aaggcgatct tcgaggacct ggggcgtatc aacgcgcaag
     3061 ggctcaccat tttggtggtc gagcagaacg tgcttcgatc gctgcacctg gcccatcgag
     3121 gctatgtgct cgagaatggg cgcgtgaccc ggtcgggggc cgggccggag ctgctcgcca
     3181 gcggccatat caagcaggcg tacctggggc tctgagggcg aaaacccgta taattcgcgg
     3241 cgctgcgggc cgagaataga ctcgtgcgtc ggttcatccg gggcttcctc gtcccgttcc
     3301 tgctgctcgc catcccgctg gggattctgg ccggcctcgg gttcatcgcg gctcaccgtc
     3361 agctcgtccg cacccgcgac gccgcggtcg cccggaccgt ggacgcggtg agcgcgctgg
     3421 tcgcggacta ccaggaatcg ttgcggcgcg agacgatcct tcttgcccgc gaccctgcgg
     3481 tggtcgaggg agcggccaag ggcgactggg cgatcctggc tcggggcgcc tccccgcgcg
     3541 tgctggccat cacgcgcgac ggactcgccg acttcatcac gatccgggac tcgcgcggca
     3601 cgcccctggt gcaggtgccg gcgagcccgc cgccgagcct ccccggcgcg cctgccgcga
     3661 ccgagccggt cctgaccctc cggctggcgg gcggccagcc gtacttcctg gtcacggcgc
     3721 cggtgcagag tccggccggg cgcgagccgc ccggcgggcc gggcagcgtg gtggccggcc
     3781 ggcgcgtcga ggggctgggc gcgctcctgg accggcttcc cgcgcgaccg gcggtggtct
     3841 tcgtcgccgg ggaccgggcg ctcgcggcca gccggagcga cctgcccacg agcggctggt
     3901 cccgctcggc cacggtgggc gccaccgacg tgggcgacga gcccttcgcg gtccgccggc
     3961 tcgtggagcc cgtcgtgacg tcgcccgacg gcggcctctg ggtggtgcta tcggtgcgcg
     4021 agttcatgcg cgccgagcac cgcctgctgc tggagttcct ggcccttctg ggggccggcg
     4081 cggtggtgct cgcgggggtg gtgctggcgt tccttccggc gggggggcgg ccgcgccggc
     4141 ctgccgggtc ggggccggcc ccggtcagcc cccgcgtggc gctcgagcgg cgcaaccgcg
     4201 agctggaagc gctgaacgcg gtgttcgcga cgatgagccg cggctccgac ctggccacca
     4261 ccgccggcga gacactcgag gtcgtgcgcg ccctcgcgcg gatggacgtc ggcgtggtct
     4321 accgcctgga tcaggacggc aatcagctcg tcctggaggg ccagagcggc gtcgatcccc
     4381 gctacctgga ccgttcccgc aaccgcccgg tggaaggctc ccgcattggt gacgccgcgc
     4441 gcaccggcca gatcatcgtc acccatctcg atgcctcccc gccctccgaa gagcagatcc
     4501 gggagatggc ggccgagcgg gctcaccgca cccagctcgc gctgccgatc ccggtcgagc
     4561 accggacctg gggcgtgatg gtgctcgtct ccaaggagcg gcgcgagttc ttgccggagg
     4621 agctgacgat cctctcggcg gtggcccagc aggtgggcct ggcggtcgag cgggcccagc
     4681 tccgcgacac ggcggcggtg cgcctcagtc gactcgaggc gcagcgcatg atcgagcggc
     4741 acatctccga gcaactcgac accgaggagc tgctggtcgt catcgcgcgc tcggcccagc
     4801 ggctgatcgg ggggacgttc tccgcgctgt acctgctgga gggcgacacc ctgcgccctc
     4861 gagcctggtc ggatgtcccg gactggatcc gcgacctccg gttcaagatc ggtaccggcg
     4921 tggccggtgc cgcgctggcc gccggccggg gcgcgctcgc caacgactac ccgagctcac
     4981 ccggcgcgat ggccgagttc atcccgttca cgagccggct gctggccgcg cccctcatgg
     5041 cgggagaccg gcccctgggc gtgatgaccg cggggcgcgg gcccggcggg ccaccgttca
     5101 ccgaggagga tctctccacg ctgaccgact tcgccacgca ggctgcggtg gcgctcgagc
     5161 acgcgcggct cttcgacgag gccacccgca acgcggccca gtaccaggcg ctcctcgagg
     5221 tcagcagcgc cgtcagctcg accctcgacg tcgaccgggt cctcgacctg gtgctgcagc
     5281 ggtgccaggc gctgctgggg gtggccgcgg tgggcgtcat gcggatcgac cgggaaaccg
     5341 gggtggtggc ctacgagcgc ggccgcgggc tgtcgggcga gttcaccacg tcgctgcgca
     5401 tgcgcctggg ggagggcacc accggccgcg cgatcgagga gcgcgtcccc gtgtggagcg
     5461 aggacgtgct gaacgacgcg gccctggcga tcagcccggc ggcacgcgag ctgatccggc
     5521 gcgagggcta ccgcgcggtc ctgtccgtcc ccctgctcac caagggcgat gcgcacggcg
     5581 cgatcgccgc ctactggtgg gagccccaca cgccgtccgc ggccgagatc tcgatcatga
     5641 ccgcgctcgc cggccaggcg gccaccgcgc tcgacaacgc ccggctgttc gcccaggagc
     5701 gcgaccgcaa ggcgtcgctg tcctcgctgc tcgagatcaa caagaagatc ggggcgctcg
     5761 tggcaccgga gagcctgctg acctccatcg cggaggaggc ggcgcgcctg ctcgatgtcg
     5821 acaacgcggg cttccgcctg ctcgacggcg acgatctggt ggtggcgggc gtcgccggcg
     5881 ccgccgccca gtccatgctg aagccgcgga tcaaggtggg cgagagcctg accggccagg
     5941 tgctcgcgac cggccaggcg ctcatgtgcg aggtcgaggc gagcgggctc ctggccgacc
     6001 acctggccgc ggaccagcag ctcggctaca cgcactatct cggggtgccg ctcctggtgg
     6061 gtgagcgggc catcggcgtg ctggccttcc gcgggcggcg ggcgttcacc gcgcgcgagc
     6121 aggagctggg ggagaccttc gccggccagg ccgccatcgc gatcgaccac tcgcgtctct
     6181 atcgcgaggc gtccgatcag gcggagcgga tgcgcgtggt ggccgagatg gggcgcgtgc
     6241 tcgtgtccac gctcgacgag gcgcgggtgc tcgaaatcgt ctccacccag gcccacgact
     6301 cgctcgggcg gctcgacatc gccatctggc tgcaggaggg gcacggcgga ccgctgcgcc
     6361 tggtcaccgg gcaggggcgg ttctccagtc cgctcgccgg gcgcgcccag ccgctcgacc
     6421 ccgacgaggg cgtggtcgga ctggccctga ccgagcgcgc gccggtctgg acggccgacg
     6481 tgctgaccga ttcccgcatc cgtctccgcc cggagtcgcg gcgctggatc gaggagatcg
     6541 gcggccggtc cctcctcgcg gtgccgctca tccgggagca cctgctgggc gcgctcgtcg
     6601 tgtaccggcc ggttggccag gtgttcagca cgcgcgaggt cgagtacctc tcggccttcg
     6661 cgaaccaggt cgcggtcgcg ctcgagaacg cccggctcta ccgggccctc gacgtgcgcg
     6721 cggcgcgcct gcgcagcctg gcccgcctcg cgcacatcgt gtcctcctcg ctcgacatgg
     6781 acgaggtgct ccgggctatc gccgaagcgg ccggcgagct gatcgcggtg cccctggcga
     6841 gcttctgggt cgcgaacgag cccgccggca cgctcgagtt ccggacctcg tccgatgcga
     6901 tggccgacga cttcccctcg aagagcctcc cgtacggcga gggcggtgtg ggctgggtgg
     6961 cgctccatcg ccagacgctc gagatcgagg agatcgcgca ggactcccgg ttcaagttca
     7021 cggagtgggc cgtgtcgcac gggctgacca gcctgctcgc ggtgccgatc gtgttccagg
     7081 ggacgctcct gggggtgctg tccctcaacg ggcgccaggc gatccggctc agtcccgacg
     7141 atcagcagct cctcgagagc ttcgtggcgc aggcgggcgt ggccatccgc aacgcggggc
     7201 tctacggcga gacccgcggc cggctcgagg agagccgcgc gctcctggag gtggccgaga
     7261 tcctcaactc cacgctggac tcgaggcggc tgctgcgcga ggtcacgatg aagatcgccc
     7321 aggtctgccg ggtggaccgc tgctccatcc agcgctggat cgatggccgc gtggtgccgc
     7381 tcatgtccca gtacgccgat ggtcatccgg accccgagac ctgggatcgg ttccgtcgca
     7441 tggccgccag cctgccgatg cggccgccgc tgcacgcacg cacggtggac acgcggcggc
     7501 ccgtgatcgt gcccgacgcc gcctccagcg agctggtccc cgcggagtgg gtggagctct
     7561 tccggctgaa gtcgaccatg accgtcccgc tgatccgaca ggacgaggtg atcggggtca
     7621 tggcgctcga ccacaccgag cgggcgacgt ccttcgagcc ctggcaggtg gacctggcca
     7681 tggccatcgc gggccagctc gcgctctcgc tggccaacac gcagctctac acccaggtgc
     7741 aggagcgcct gcgggagacg acggccctgc tggccgtcgg ccgcgctctc tctcagccgg
     7801 agccgaccgc ccacgtcatg cgcacggtgg cgcgcgaggt ggcccaggcc ttcggcgccg
     7861 acatggtcgg ggtctacagc ctcgacccca agcacgaggc gctcgtgccc accgcgggct
     7921 accacgtgcc caagcacctg ctcgagcact tcctgaccca gcccttcacg ctcgcccggt
     7981 ttcccgccct gacccaggtc tggcgtgagg ggcgggccgc gtggtcctcg gacgccaacg
     8041 cggacccgcg cttcgaccgc ggggtgctcg agggcatcga cgcccattcc gtcctcttcg
     8101 ccccgaccac cgtgcggggc gagccggtcg gtgcgctgtt cctggtctgg tggcagaccg
     8161 ggcgcgagtt cagcgagtcg gaggtgcggc tcctcgaggg cgtggccgcc caggtcggcc
     8221 tcggcatgga gaacgcggag ctggcgcggc agaccgagct gaagctccag gagacggaga
     8281 cactcctcgc ggttggccag acgctcgcct cgaccctcga cgtggatacc ctgacgcgcc
     8341 agttcctgcg ccacgtggcg cacggcctcg gcgcggacag cacgggcatg tggctgctgg
     8401 gggacgacgg ggagtggatg gcgccgctgg cgggctacca cgttcccgcg gatcgcctcg
     8461 atgcgctccg aggcctgcgg ctctcgatcc gggaccacga gttctaccgc gaggcggccg
     8521 agcagcggcg gtccgtcatc tccaccgacg gcgcccacga tccacgcatc cccgaggaga
     8581 ttcgccggcg cgcgccggtc cgcacgcacc tcttcgtgcc gatcttcgcg caggagcaga
     8641 tgatcggcgg cttctccgcc aactggtggg acaaggtccg cgagctctcg gacgccgagc
     8701 gccgtctgat ggaggcggtg gcgagccagg cgggggtggc cctgcagaac gcgcgcctct
     8761 tccaggacaa ccagcgccgg gtgcgggagc tgtcggtgct ccacgagctg tcgcgctcgg
     8821 tcaccggcca gctcgaccag gccggcatcc tggacacgct gcagcagcag gtgccgcgcg
     8881 tcctggacgt ccggcacatg tcggcgatgt tgctggacga ggagcacgag cggctggacg
     8941 tggtgctgcg cgtgcgggac gggcgccgct gcgacgagga cgagccgcgc tcctacccgc
     9001 ttgaccaggc cgggctttcc agtctcgtct tcaacagcgg ccggccgatc cggaccgcgg
     9061 actacgcggg cgagtgccga cggcagggcg tgacgccggt cgccagcgcc atggacatgc
     9121 cgcacgccat cgtcgtgccc atgacggcgg gcgaccgcgt catcgggctg ctgtcgctgc
     9181 gcagccccga tcgcgcgttc accgacgcgg atgagcggct cctcgtcaac atcgcccagc
     9241 tcgtcgcgct catgctccgc agcgcgcgcc tgtacgagga gcgcacgcgc gccctcggcg
     9301 agctgggcgc cgcccaggac cagctcgtgc gcaccgagaa gctccgcgcg ctcggcgaga
     9361 tggcctcggg agtggcccac gacttcaaca acctgctcgc ctccatcctg ggccgcgccc
     9421 agctcctgct cgagcgcatc caggacgtca agctgcgcca gtggctcaag gtcatcgagc
     9481 gcgcggccat ggacggcgcc cgcaccgtgc gccggctgca ggacttcacg ggcatccgac
     9541 gcgaccagcc cgcggtggac gtggacctca accaggtcct ccagcaggtt ctcgagacca
     9601 ccgagtcgat ctggcggcag gaccgtggcc ggagcggcgg ggccgagatc caggtggaga
     9661 ccgccctggc cgcggagctg ccgccggtgg cgggcgatcc ggccgagctg cgcgaggcct
     9721 tcaccaacct ggtgctcaac gcggtcgacg cgatgccgaa gggcggcacc ctgaccctgc
     9781 gcacccgggc cgccgagggc caggtcgagg tcgaggtgcg cgacaccggg acgggcattc
     9841 ccgagcacat ccgcgagaag atcttcgacc cgttcttcac gaccaagggg ccgaagggca
     9901 ccggcctcgg cctctcgatg gcctatggta tcctccagcg ccacaacggg cggatcacgg
     9961 tcgagagcga agcgggccac ggcacgacct tccgcctgct gttccccgcc gtggtctcgg
    10021 gctcggtggc ggatgcggtc ccgccgctca cgccggcgcc cggcgctctg gtctcgctgc
    10081 actgcctggt ggtggacgac gagcaggagg tgggcgaggt ggtggccgac atcctgacca
    10141 ccgcggggca caccgcggtg accgtgggga acggcaagga aggcgtggac cgactcggcg
    10201 tcgagcggtt cgacgtggtg ttcacggacc tggcgatgcc cggcatgacg ggctggcagg
    10261 tggcgcgcgc ggtcaaggac cgggccccgg gggtagcggt ggtgatgatg agcgggttcg
    10321 gggtcgaggt cgcgccggag gatctccaca cccacggcgt cgatctggtg ctggccaagc
    10381 cgctccagat ccacgacgtg ctgcgcgcgg tggaagcgat ccgcc
//
LOCUS       DASUFA010000453         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_221803, whole genome shotgun sequence.
ACCESSION   DASUFA010000453 DASUFA010000000
VERSION     DASUFA010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221803"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFX87_16400"
     CDS             <1..209
                     /locus_tag="VFX87_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX4996563.1"
                     /translation="ELVYGRFEELTRSYFKEVIRQAQGRRCDAVILGCTEFPLLIGDA
                     DSPLPTLDSTRILARAALRAAVGP"
     gene            320..742
                     /locus_tag="VFX87_16405"
     CDS             320..742
                     /locus_tag="VFX87_16405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4996564.1"
                     /translation="MSGPRHRRIFPARRDALPEVEAFLAEVCAAAGLDREMCLRVTLL
                     VEELFTNTVVHGHGGDSDAPVRLDCEVAPGRVTLVYEDTGPPHDPFAAVASPDPDAEV
                     QDRPVGGLGVLLVSAMARHVEYRRADDRNRISLVFGSA"
     gene            787..2334
                     /locus_tag="VFX87_16410"
     CDS             787..2334
                     /locus_tag="VFX87_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4996565.1"
                     /translation="MNWKSEMDELKRREEFAEGLGGAERVKRQHDGGRYTIRERIDRL
                     VDAQSFHEIGKIAGKASYDANNDLKEVTPSNFVFGRARLDGRPVVVGGDDFTVRGGSA
                     DATIKGKHNQCEQMANELRLPLIRIVEGSGGGGSVKTIETTGRANVPGVAGWEWVVNS
                     IGTVPRVALGLGSVAGLGAAHLAAAHFSVMVKDTSALFVAGPPVVERLGQKLTKNELG
                     GWQIQLKAGAVDQAVNTEEEAFAAARRFLSYLPSSIYELPPRGPQTDDRERREEWLGD
                     AVPRDIRKPYKMRAIVEAVVDRGSFFEIQPLYGRAIITGLARLDGWPVAVLASDPYFY
                     GGAWTADTCQKVERFVDFAQTFHLPVVYLVDCPGFLIGLEAEQTGTIKQGVRAMSAIW
                     QSTMPWCAVIVRNVFGVAGAAHKPGARYGPRYAWPSGRWGSLPLEGGIEAAYRADIDA
                     AADPAAKMIEIGERLQKLRSPFRSAEAFWIEEIIDPRATRPLLCEFAELAAPLREPGP
                     STHRMRP"
     gene            2414..>2506
                     /locus_tag="VFX87_16415"
     CDS             2414..>2506
                     /locus_tag="VFX87_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxy-D-gluconate 3-dehydrogenase"
                     /protein_id="HEX4996566.1"
                     /translation="MAYKAFDLTGKTAIVTGGNSGIGLGMAEAMA"
BASE COUNT          353 a          881 c          900 g          372 t
ORIGIN      
        1 atgagctcgt gtacggccgc ttcgaggagt tgacccggtc ctacttcaag gaggtcatcc
       61 ggcaggcgca gggtcgccgg tgcgatgcgg tgatcctcgg ctgcacggag tttcccttgc
      121 tcatcggcga cgcggactcg ccgcttccca ccctcgactc cacccggatc ctcgcgcggg
      181 ccgccttgcg cgcggcggta ggcccgtagc ctctcgggcc cttgccttgc tgaggcgctg
      241 gcgctcggac gcctgacgtc aggcctatcg tctcgagttt gacggcggac gcggtcccgg
      301 cggcccttac aatagcccaa tgagcggccc ccgccaccgc cggatcttcc ccgcccgccg
      361 cgacgccctg ccggaggtcg aagccttcct cgccgaggtg tgcgcggccg ccggtctcga
      421 tcgggaaatg tgcctgcggg tcacgctcct ggtggaggag ctcttcacca acacggtggt
      481 ccacgggcac ggcggggaca gcgacgcgcc ggttcggctc gactgcgagg tggcgcccgg
      541 ccgcgtcaca ctcgtctacg aggacaccgg cccgccgcac gatcccttcg cggcggtggc
      601 gagccccgat ccggacgcgg aggtgcagga ccgcccggtg ggcgggctcg gcgtgctcct
      661 cgtctccgcc atggcccggc acgtggaata ccggcgggcg gacgaccgga atcgcatctc
      721 gctcgtgttc ggctccgcct aggcatcccg cgcgctccct gctaccatcc ccgcggaggt
      781 ctccccatga actggaagtc cgagatggac gagctgaagc gccgcgagga gttcgccgag
      841 ggtctgggcg gcgcggagcg ggtcaagcgg cagcacgacg gcgggcgcta cacgatccgc
      901 gagcgcatcg accggctggt ggatgcgcag agcttccacg agatcggcaa gatcgccggc
      961 aaggcctcct acgacgccaa caacgacctg aaggaggtga cgccgtccaa cttcgtcttc
     1021 ggccgcgcgc ggctcgacgg ccgcccggtg gtcgtgggcg gcgacgactt cacggtgcgg
     1081 ggcggctcgg cggatgcgac gatcaagggc aagcacaacc agtgcgagca gatggccaac
     1141 gagctgcggc tgccgctgat ccgcatcgtc gagggctcgg gcggcggcgg ctcggtgaag
     1201 accatcgaga ccaccgggcg cgccaacgtg cccggcgtcg ccggctggga gtgggtcgtg
     1261 aacagcatcg gcacggtgcc gcgcgtggcg ctgggtctgg gctcggtggc cggcctcggc
     1321 gccgcgcatc tcgcggccgc gcacttctcg gtcatggtca aggacacgtc ggcgctcttc
     1381 gtggcgggcc cgcccgtcgt ggagcggctc ggccagaagc tcaccaagaa cgagctcggc
     1441 ggctggcaga tccagctcaa ggcgggtgcg gtggaccagg cggtgaacac ggaggaggaa
     1501 gcctttgccg cggcgcgccg gttcctctcc tatctgccct cgtccatcta cgagctgccg
     1561 ccgcgcggcc cgcagaccga cgaccgggag cgccgcgagg agtggctcgg cgacgcggtg
     1621 ccacgcgaca tccgcaagcc gtacaagatg cgcgcgatcg tggaggcggt ggtggatcgc
     1681 gggtcattct tcgagatcca gccgctctac ggccgcgcca tcatcaccgg gctggcccga
     1741 ttggacggct ggccggtggc ggtcctggcc agcgatccct acttctacgg cggcgcctgg
     1801 accgccgaca cctgccagaa ggtcgagcgc ttcgtggact tcgcgcagac gttccatctg
     1861 ccggtggtct acctggtgga ctgcccgggc ttcctgatcg gcctcgaggc ggagcagacc
     1921 ggtacgatca agcagggcgt gcgcgcgatg tccgcgatct ggcagtccac catgccgtgg
     1981 tgcgcggtca tcgtccgcaa cgtgttcggg gtggcgggcg cggcccacaa gccgggggcg
     2041 cgctacggcc cgcgctacgc gtggccgtcg gggcggtggg gctcgctgcc gctcgagggc
     2101 ggcatcgagg ccgcctaccg cgccgacatc gacgcggccg ccgatccggc cgcgaagatg
     2161 atcgagatcg gggagcggct gcagaagctc cgctccccgt tccgctcggc ggaggccttc
     2221 tggatcgagg agatcatcga cccgcgcgct acccgcccgc tgctctgcga gttcgccgag
     2281 ctggccgcgc cgctgcgcga gccggggccg tccacccatc gcatgcggcc ctgagcgcgc
     2341 tcggtcaccc cacccctcac cctgccctct cccctgaggg gagagggatg tacggatgaa
     2401 aggctcttca gccatggcgt acaaggcctt cgatctcacc ggcaagaccg cgatcgtcac
     2461 cggcggcaac agcggcatcg gcctcggcat ggcggaggcg atggcg
//
LOCUS       DASUFA010000454         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_569204, whole genome shotgun sequence.
ACCESSION   DASUFA010000454 DASUFA010000000
VERSION     DASUFA010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569204"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VFX87_16420"
     CDS             <1..1135
                     /locus_tag="VFX87_16420"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4996567.1"
                     /translation="CTQMLAWLGAEVIKIEPPTGEPGRTALSDKRGEDAWFFLLLNSC
                     KKGVTLNLKSDRGRAMFLDLVKTADVLVENMGPGAMDRLGLGWEALHRLNPRLIAASV
                     KGFGGGGPYADYKSFEWIAQAMGGAMSMTGSPDGPPTKAIGGLADTGAGLHTAIGILA
                     AIIQRQATGVGQQVEVAQQESVVNLLRIHLRESYATGKPAPRQGNRSAAAAPSNIYRC
                     RPGGPNDYVFIHCATVDMWKSLTKIVGRPEMADDTRYHDRRDRVQIVDEIDAMIEAWT
                     EKRTKFEVMEIMAGAGVPCGAVLDSTEILADPHLRKRRFIVDLEHPRRGPYPMPGNPV
                     RLSDSPTEVGRSPLLGEHNADVYGRLLGYKPDQIESLRRDGVI"
     gene            1201..2136
                     /locus_tag="VFX87_16425"
     CDS             1201..2136
                     /locus_tag="VFX87_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4996568.1"
                     /translation="MSDNHAVAVDPAAPGRLALRAVPDPTPDRGEAIIRVRAISLNRG
                     EVRRSTLAAAGWRPGWDLAGHVERAAADGTGPQVGARVVGLLPEGAWAERVAVPTHAL
                     AELPEKVTFSQAATFPVAGLTALHALSKNGPLLARRVLVTGATGGVGDFAVQLARLGG
                     AHVTATARRADQVPALRQLGAHEVTVGDEVPPSPKYDLIVESVGGRTLGTALAALQRG
                     GVCVTLGVSAATEVTFDARTFFATGRSGLYGLYLFTELGSEPASIGLRRLADLVAAGQ
                     LAPHISVERPWTEIAQVAADLIARKYPGKAVLTVG"
     gene            2142..2612
                     /locus_tag="VFX87_16430"
     CDS             2142..2612
                     /locus_tag="VFX87_16430"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEX4996569.1"
                     /translation="MPRLDRLPPLNRNNLLMLPVQVNDTAPYTRLATPLAACRLAIVT
                     TAGLHRRGDRPFGPGDQSYRVIPSATPAADIVQSHTSLGFDRVPTMRDLNISFPVDRL
                     RELTAKGELGGAGPHHYSFMGAQRDLKRIQEETGPEVGRRLRDEGVDVALITPT"
     gene            2637..3335
                     /locus_tag="VFX87_16435"
     CDS             2637..3335
                     /locus_tag="VFX87_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996570.1"
                     /translation="MLARALETEGIATTSISMVREHTEKIKPPRALFVPFPFGYALGR
                     PDDPDLQHRVLRAALDLLAEPAGPVLHDFPDDAEPGDQPAAPAQASGIAPTAGVPDDP
                     ALETAQMRQYHEQWLARSGGRTAFGLCGVPATRFRGVVRFLQAFAAGEDATMEERPAE
                     VPLPNFIRYCADDWKALYFEGRLAMKPGAPGDDVTRWFWAETAAGQVLRRVRDRLDAS
                     DDPRSKAAAFGVAR"
BASE COUNT          504 a         1388 c         1163 g          464 t
ORIGIN      
        1 ctgcacccag atgctggcgt ggctcggggc ggaggtcatc aagatcgagc cgcccaccgg
       61 cgagcccggc cgcaccgcgc tgtccgacaa gcgcggcgag gacgcgtggt tcttcctgct
      121 gctcaactcc tgcaagaaag gggtcacgct caacctgaag tcggaccgcg gccgggccat
      181 gttcctcgac ctggtgaaga ccgccgacgt gctggtcgag aacatgggcc ccggcgcgat
      241 ggaccggctc ggcctcggct gggaggcgct gcaccggctc aacccgcggc tgatcgcagc
      301 ctccgtgaag ggcttcggcg gcggcgggcc atacgccgac tacaagagct tcgagtggat
      361 cgcccaggcc atgggggggg ccatgagcat gaccggctcg cccgacggcc cgcccacgaa
      421 ggccatcggc ggtctcgccg acaccggcgc gggcctgcac accgccatcg gcatcctggc
      481 cgccatcatc cagcgccagg ccaccggcgt gggccagcag gtggaggtgg cccagcagga
      541 atcggtcgtg aatctcctcc gcatccatct ccgcgagagc tacgccaccg gcaagccggc
      601 cccgcgccag ggcaaccgct ccgccgccgc ggcgccgtcc aacatctacc gctgccggcc
      661 cggcgggccc aacgactacg tgttcatcca ctgcgccacc gtggacatgt ggaaatcgct
      721 tacgaagatc gtgggccgcc ccgagatggc cgacgacacc cgttaccacg accggcgcga
      781 ccgcgtgcag atcgtcgacg agatcgacgc gatgatcgag gcgtggaccg agaagcgcac
      841 caagttcgag gtcatggaga tcatggccgg cgccggcgtc ccctgcggcg cggtcctcga
      901 ctccaccgag atcctggccg acccgcatct caggaagcgc cgcttcatcg tggatctcga
      961 gcacccgcgc cgcggcccct acccgatgcc cggcaacccg gtgcgcctct ccgactcccc
     1021 caccgaggtc ggccgatcgc ccctcctggg cgagcacaac gccgacgtct acggccgcct
     1081 cctcggctac aagcccgacc agatcgaatc cctacgccgc gacggcgtca tctaatcccc
     1141 cacccaccct ctcccctcag gggagagggt ggggtgaggg gtggtctcca ggagaacaac
     1201 atgagcgaca accacgccgt agcagtcgac cccgccgccc ccggccgcct cgccctccgc
     1261 gcggtccccg acccgacccc cgaccgcggc gaagccatca tccgcgtacg cgcgatctcg
     1321 ctcaaccgcg gcgaggtccg ccgctctacc ttggccgcgg ccggctggcg ccccggctgg
     1381 gacctggccg gccacgtgga gcgcgccgcc gccgacggca ccggaccgca ggtcggcgcg
     1441 cgcgtcgtcg ggctcctgcc ggaaggcgcc tgggccgagc gcgtggccgt gcccacgcac
     1501 gcgctcgccg agctgcccga gaaggtgacg ttctcgcagg ccgccacctt cccggtggcc
     1561 ggcctcaccg ccctgcacgc cctcagcaag aacggcccgc tcctcgcccg ccgcgtgctc
     1621 gtgaccggcg cgaccggcgg cgtcggcgac ttcgcggtgc agctcgcgcg cctgggcggc
     1681 gcccacgtca cggccaccgc ccgccgcgcc gaccaggtcc ccgcgctccg tcagctcggc
     1741 gcgcacgagg tgacggtcgg cgacgaggtc ccgccctcgc ccaagtacga tctcatcgtc
     1801 gagtcggtgg gcggtcgcac cctcggcacc gcgctcgcgg cgctgcagcg aggcggcgtc
     1861 tgcgtcactc tcggcgtctc cgctgccacg gaggtcacct tcgacgcccg caccttcttc
     1921 gccacgggac gctccgggct gtacgggctc tatctcttca ccgagctcgg cagcgagccc
     1981 gcgtcgatcg gcctgcgccg cctcgccgac ctggtggccg cgggccagct cgccccgcac
     2041 atcagcgtgg agcggccgtg gaccgagatc gcccaggtgg cggccgatct gatcgcacgg
     2101 aagtacccgg gcaaggccgt gctgacggtg ggctagatcc gatgcccaga ctggaccggc
     2161 tgcccccgct caaccgcaac aacctgctga tgctgccggt ccaggtcaac gacaccgcgc
     2221 cgtacacgcg cctggccacg cccctcgccg cctgccgcct ggccatcgtc accaccgccg
     2281 gcctgcatcg acgcggcgac cggccgttcg ggcccggcga ccagagctac cgcgtgatcc
     2341 cgtcggcgac gccggccgcc gacatcgtcc agagccacac cagcctcggc ttcgatcgcg
     2401 tgccgacgat gcgcgacctg aacatctcgt tcccggtgga ccgcctccgc gagctgaccg
     2461 cgaagggcga gctgggtggc gcgggaccgc accactactc gttcatgggc gcgcagcgtg
     2521 atctcaagcg catccaggag gagaccggac ccgaggtcgg ccgccggctc cgcgacgagg
     2581 gcgtcgacgt cgccctgatc accccgacct gacccttctg cacgcacacc ggaggtgtgc
     2641 tggcccgcgc gctcgagacc gagggcatcg ccaccacgtc gatcagcatg gtccgggagc
     2701 acaccgagaa gatcaaaccg ccccgcgccc tcttcgtacc gtttcccttc ggctacgcgc
     2761 tgggccgccc ggatgacccg gatctccagc atcgcgtgct gcgcgccgcg ctcgacctgc
     2821 tcgccgagcc cgcgggcccg gtgctccacg acttccccga cgacgcggag ccgggagatc
     2881 agccggccgc acccgcgcag gcatcgggca tcgcccccac cgccggcgtg ccggacgacc
     2941 ccgcgctgga gaccgcgcag atgcgccagt accacgagca gtggctcgcg cgcagcggcg
     3001 ggcgcaccgc cttcggtctc tgcggcgtgc ccgccacccg gttccgcggg gtggtgcgct
     3061 tcctccaggc cttcgcggcc ggcgaggacg ccaccatgga ggagcgtccg gcggaggtcc
     3121 cgctgccgaa cttcatccgc tactgcgcgg atgactggaa ggcgctctac ttcgagggcc
     3181 gcctggccat gaagccgggc gcgcccggcg acgacgtcac gcggtggttc tgggccgaga
     3241 cggcggccgg ccaggtcctg cgccgcgtcc gcgaccgcct ggacgcctcc gatgacccgc
     3301 gctcgaaggc ggccgccttc ggggtggcgc ggtaggcgcg cctctcgtcg agtcgagccc
     3361 cttcccgcga gctctcgacc cctcaccctg ctctctcccc tctggggaga gggtgagctc
     3421 gggcctaccg cgctactcgt taagccacac cgtatcgagc tggcagttca ggaagtggct
     3481 cggcgtgatc gtccagccgc gcaccttgga gaggtgcgg
//
LOCUS       DASUFA010000455         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U1367 k141_223563, whole genome shotgun sequence.
ACCESSION   DASUFA010000455 DASUFA010000000
VERSION     DASUFA010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961519
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,291
            CDSs (total)                      :: 3,280
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223563"
                     /isolate="SMAG_U1367"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..830)
                     /locus_tag="VFX87_16440"
     CDS             complement(39..830)
                     /locus_tag="VFX87_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4996571.1"
                     /translation="MATSLRVAAVGDLHCTRTCEGAFQPLFAKIAESADVLVLCGDLT
                     DYGTPEEARVLAKELSAMKLPKLAVLGNHDFESTASDEVARILTDAAGVSVLDGTAVE
                     VQGVGFAGAKGFAGGFGARALQSWGEGPVKAFVAAAVEETLKLESALARLRTTGRVAL
                     LHYAPIGATVDGEPVEIYPFLGSSRLEEPINRYRPDAVFHGHAHRGQLEGATAAGVPV
                     YNVALPLLRRRFPDRPPFRVVEVPVGEEASGHPAPGGAAAAGARA"
     gene            complement(927..1226)
                     /locus_tag="VFX87_16445"
     CDS             complement(927..1226)
                     /locus_tag="VFX87_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996572.1"
                     /translation="MTLTQRSRTSPARARRWAVAGAACLILAHALFWLLLNPVNQVFA
                     AWTSTAVPPDWPRLRDQWEFTHAARAGLFLLGFCALLAAVLAARSGAERRGETTG"
     gene            1333..2160
                     /locus_tag="VFX87_16450"
     CDS             1333..2160
                     /locus_tag="VFX87_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4996573.1"
                     /translation="MRSALAWPWALLCATILWVVTSVCPDAEAGAVTVRYLEGVTHGF
                     LTLRSTAGDLLAEGDLLQVVRPEGVDSRLVFHFKDGSLYDETVVFTQSKVFTLVRYRL
                     QQRGPSFPEPVEISVDRERDSKGRYTVKSRRAGDEAETATGEIELPSDVYNGMFIMIL
                     RNLPKGAGETVHVLAFTPKPTLIQVELIPQAAETVLAGEQRRVPVTRFVLKPKLGLLR
                     GAVAALFGKTPPAYHCWIVTADPPAFVAIDGPLYTGGPIWRFEAVSPRGPARPAVRR"
     gene            complement(2174..>3667)
                     /locus_tag="VFX87_16455"
     CDS             complement(2174..>3667)
                     /locus_tag="VFX87_16455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4996574.1"
                     /translation="EYRGAQERFVIKPGRGAGGLVLESGRPFRSDNMAEDSRITQAHG
                     DFVEIEGLVATLVVPIMVEGRVEGLLYVHRRTPKPFNDRTETVLRQLAEYAAIAIHNM
                     RMLAHEHQMRAEAESASRTKDEFLAMLSHELRSPLQPLLNWAYLLRSPNLDPASAERA
                     LDAIERSTKTLGQLIEDLLDVSRIVTGKLRLQPRPVRLPAVVRAAIEAVEPAALAKTV
                     TLEVRIEPGVPTVMGDPDRLQQVLWNLLSNGIKFTPKGGRVTVTVAGRDSEVMLTVAD
                     TGAGIKREFLPHVFERFRQGESSTNRAYGGLGLGLAIVRHLVELHGGSVAVQSEGEGQ
                     GATFAVRLPVAAAIRTAEHAPAAVSTDGPVRQSLSGLRLLIVDDEADAREVMRFMLER
                     GGAQVRIADSAVQALDAIREERPDLLISDIGMPIEDGYVLVRRLRAMEEGLGRRLPAI
                     ALTAYASEEDTRRALAAGFDAHLSKPVDPARLIDIAAGLAGGRRGSS"
BASE COUNT          514 a         1344 c         1298 g          511 t
ORIGIN      
        1 gcggggcgcg cgcccggcgg cggtagcctc cacgggggtc atgcgcgagc ccccgcggcg
       61 gccgcgcccc cgggcgcggg gtggccggac gcctcctcgc cgaccgggac ttcgaccacg
      121 cgaaagggtg gccggtcggg aaagcgtcgg cgcagcagcg gcagggccac gttgtagacc
      181 ggaacgcccg ccgcggtggc gccctcgagc tggccgcgat gggcgtgccc gtggaagacg
      241 gcgtcgggcc ggtagcgatt gatcggctcc tcgaggcggc tcgagcccag gaacgggtag
      301 atctcgacgg gctccccgtc cacggtggcg ccgatgggcg cgtagtgcag gagcgcgacc
      361 cggcccgtcg tgcggaggcg ggccagcgcc gactcgagct tgagcgtctc ctcgaccgcg
      421 gcggccacga acgccttcac cgggccttca ccccacgact gcagcgcgcg cgccccgaat
      481 ccgcccgcaa accccttggc gcccgcgaag ccgacaccct gcacctccac cgcggtgccg
      541 tccagcaccg agacgccggc cgcgtcggtg agaatgcgcg ccacctcgtc ggacgcggtc
      601 gactcgaagt cgtggttgcc gagcaccgcg agcttcggca gcttcatcgc actgagctcc
      661 ttggcgagca cgcgcgcttc ctccggcgtg ccgtagtcgg tgagatcgcc gcagagaacg
      721 agcacgtctg cgctctccgc gatcttcgcg aagagtggct ggaaggcgcc ctcacaggtc
      781 cgggtacagt gcagatcacc gacggcggcg acacgaagtg acgtggccat gtaggggcgg
      841 gttgcaaccc ccatgcccac gtggccgacc cccaacccct ctgataccgg cccctggctg
      901 agcggaggcc tgccggtcgg aagctcctac ccggtagttt cgcctcgacg ctccgccccg
      961 gaccgggccg caagcaccgc cgccaggagc gcgcagaagc cgagcaggaa gagcccggcg
     1021 cgggccgcgt gcgtgaactc ccactggtcg cggagccgcg gccagtcggg aggcaccgcg
     1081 gtcgacgtcc aggccgcgaa cacctgattc accgggttga ggaggagcca gaacaatgcg
     1141 tgcgccagga tcaggcacgc ggcgcccgcc accgcccagc gacgcgcgcg ggcgggcgat
     1201 gtgcgcgatc gttgcgtcag cgtcacgtag acggccaggc ggtcatgccc ttcgccgcag
     1261 caaccggaat gccagggcga gtcatcctta ccggcactca cggctggcaa cgagcttgca
     1321 gatcacaacc tcatgcggag tgcgctcgcg tggccctggg ctctcctgtg cgcgaccatc
     1381 ctgtgggtcg tcacgtcggt ctgcccggac gcggaggccg gtgcggtaac ggtccgctat
     1441 ctcgaaggcg tcacccacgg cttcctcacg ctccgctcga ccgcggggga cctgctggcc
     1501 gagggcgacc tgctgcaggt cgtccgcccc gagggtgtgg actcgcgcct cgtgttccac
     1561 ttcaaggacg gctcgctcta cgacgagacg gtggtgttca cgcagagcaa ggtcttcacc
     1621 ctggtgcgct accgcctgca gcagcgcggc ccgagctttc ccgagccggt ggagatctcg
     1681 gtggaccgcg agcgcgacag caagggccgc tacacggtca agtcgcggcg cgcgggcgac
     1741 gaggccgaga ccgcaaccgg cgagatcgag ctgccttccg acgtctacaa cggcatgttc
     1801 atcatgattc tccggaacct gcccaagggc gcgggagaga ccgtgcacgt gctcgcgttc
     1861 acgccgaagc cgaccctcat ccaggtcgag ctgatcccgc aggcggcgga gacggtgctc
     1921 gcgggcgagc agcgccgcgt gcccgtcacg cgcttcgtgc tcaagccgaa gctcgggctg
     1981 ctccggggcg ccgtggccgc gctgttcggg aagactccgc ccgcctatca ctgctggatc
     2041 gtgaccgccg acccgcccgc cttcgtggcg atcgatggcc cgctctacac cggcgggccc
     2101 atctggcgct tcgaggcggt gagcccgcgg ggccccgcgc ggccggcggt gcgccgctga
     2161 gcgggccggc ttttcaggag cttccgcgcc ggccgcccgc cagtccggcc gcaatgtcga
     2221 tgaggcgggc cggatccacc ggcttcgaga ggtgcgcgtc gaagcccgcg gcgagggcgc
     2281 ggcgggtgtc ctcctcgctc gcgtacgcgg tcagagcgat cgcgggcagg cgccggccga
     2341 ggccctcctc catcgcgcgc aggcggcgta ccagcacgta gccgtcctcg atcggcatgc
     2401 cgatgtcgct gatgaggaga tcggggcgct cctctcggat cgcgtcgagg gcctggaccg
     2461 ccgaatccgc gatccgcacc tgcgcgccgc cgcgctcgag catgaagcgc atcacctcgc
     2521 gggcgtccgc ctcgtcgtcc acgatgagca ggcggaggcc cgagaggctc tggcgcaccg
     2581 gcccgtcggt gctcaccgcg gcgggcgcgt gctcggcggt gcggatcgcc gccgccaccg
     2641 gcagacggac ggcgaacgtc gccccctggc cctccccctc gctctgcacc gccacgctgc
     2701 ccccgtgcaa ttcgaccagg tgccgcacga tggccaggcc gagcccgagg ccgccgtagg
     2761 cccggttggt gctgctctcc ccctggcgga agcgctcgaa gacgtggggc aggaactcgc
     2821 gcttgatgcc cgccccggtg tccgccaccg tgagcatcac ctccgagtcg cggccggcca
     2881 ccgtcaccgt cacccgcccg cccttcggcg tgaacttgat cccgttggag agcaggttcc
     2941 agagcacctg ctggagccgg tccggatcac ccatcaccgt cggcacaccg ggctcgatcc
     3001 gcacctcgag cgtcaccgtc ttggcgagcg cggccggctc caccgcctcg atcgccgcgc
     3061 gcaccaccgc gggcaggcgc accgggcggg gctgcagccg cagcttcccg gtgacgatgc
     3121 gcgagacgtc gaggagatcc tcgatgagct ggcccagcgt cttggtgctg cgctcgatgg
     3181 cgtccagcgc gcgctccgcg ctcgccgggt cgagattggg cgagcgcagc aggtaggccc
     3241 agttgagcag gggctggagc gggctgcgga gctcgtggga gagcatggcc aggaactcgt
     3301 ccttggtccg gctcgcgctc tccgcctccg cccgcatctg gtgctcgtgg gccagcatcc
     3361 gcatgttgtg gatggcgatg gccgcgtatt ccgcgagctg gcgcaggacg gtctcggtgc
     3421 gatcgttgaa gggcttcggc gtgcgccggt ggacgtagag caagccctcc acgcggccct
     3481 ccaccatgat cggcaccacc agggtggcca cgaggccctc gatctcgacg aagtccccgt
     3541 gggcctgcgt gatccgcgag tcctccgcca tgttgtcgct gcggaacggg cggcccgact
     3601 cgagcacgag cccgccggcg ccgcggcccg gcttgatgac gaagcgctcc tgggctccac
     3661 ggtactc
//
